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Sample records for con soya perenne

  1. CARACTERÍSTICAS DE LAS BEBIDAS CON PROTEÍNA DE SOYA

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    Vanegas Pérez, Luz Stella; Restrepo Molina, Diego Alonso; López Vargas, Jairo Humberto

    2009-01-01

    Se describe la soya desde el punto de vista bromatológico, nutricional y funcional, igualmente se desarrollan los aspectos asociados a los beneficios para la salud que han obtenido diversos autores mediante estudios clínicos, identificando los componentes responsables de esta acción. Posteriormente se desarrollan las características de funcionalidad fisicoquímica de la proteína de soya y su incidencia en los atributos sensoriales de las bebidas con soya, incluyendo las deficiencias que ésta p...

  2. CARACTERÍSTICAS DE LAS BEBIDAS CON PROTEÍNA DE SOYA CHARACTERISTICS OF THE DRINKS WITH SOYBEAN PROTEIN

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    Luz Stella Vanegas Pérez; Diego Alonso Restrepo Molina; Jairo Humberto López Vargas

    2009-01-01

    Se describe la soya desde el punto de vista bromatológico, nutricional y funcional, igualmente se desarrollan los aspectos asociados a los beneficios para la salud que han obtenido diversos autores mediante estudios clínicos, identificando los componentes responsables de esta acción. Posteriormente se desarrollan las características de funcionalidad fisicoquímica de la proteína de soya y su incidencia en los atributos sensoriales de las bebidas con soya, incluyendo las deficiencias que ésta p...

  3. Obtención de una formulación de barniz con base en aceite de soya a escala de laboratorio

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    Bravo Silva, Ana María; Cardona Arango, Rafael

    2009-01-01

    En este proyecto se desarrollaron cuatro formulaciones de barniz para madera con base en Aceite de Soya, por medio de un proceso de mezcla a escala de laboratorio; este proceso inició con la refinación de Aceite de Soya crudo, el cual fue desgomado y neutralizado, a su vez fue caracterizado tanto en su estado crudo como refinado, para esto se determinó su índice de acidez, yodo y saponificación respectivamente. Las cuatro formulaciones cuentan con Aceite de Soya, aceite de linaza, aditivos se...

  4. CARACTERÍSTICAS DE LAS BEBIDAS CON PROTEÍNA DE SOYA CHARACTERISTICS OF THE DRINKS WITH SOYBEAN PROTEIN

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    Luz Stella Vanegas Pérez

    2009-12-01

    Full Text Available Se describe la soya desde el punto de vista bromatológico, nutricional y funcional, igualmente se desarrollan los aspectos asociados a los beneficios para la salud que han obtenido diversos autores mediante estudios clínicos, identificando los componentes responsables de esta acción. Posteriormente se desarrollan las características de funcionalidad fisicoquímica de la proteína de soya y su incidencia en los atributos sensoriales de las bebidas con soya, incluyendo las deficiencias que ésta presenta, para finalmente señalar los aspectos que logran mitigar este tipo de defectos mediante el uso de ingredientes complementarios.Soy is described since its bromatologic, nutritional and functional aspects, as well as, developed associated matters to nutritional benefits carried by means of clinical studies from different authors, for the identification of the responsible components. Subsequently, physical and chemical soy protein functionality characteristics, and their impact on soy beverages sensorial are evaluated; soy protein deficiencies, are also discussed. Finally, aspects that get mitigate the soy limitations, are pointed out with a through analysis of complementary ingredients use.

  5. Biodisponibilidad de lisina en dos pastas de soya con diferente nivel de actividad ureásica en pollos de engorda

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    Arturo Cortes Cuevas

    2014-01-01

    Full Text Available Para conocer la biodisponi bilidad de lisina para pollos de dos pastas de soya (PSA y PSB, con diferente actividad ureásica (AU 0.11 y 0.17 unidades de incremento de pH, se realizó el presente experimento. Se utili zaron 210 pollitos Ross de 1 a 21 días de edad, los cuales se distribuyeron al azar en 7 tratamientos con tres repetici ones. Los tratamientos fueron: 1 dieta basal sorgo-soya-ajonjolí (deficiente en lisina, 2 dieta basal + 0.0 5% de L-lisina, 3 dieta basal+0.10% de L-lisina, 4 dieta basal+0.05% de lisina a partir de PSA, 5 dieta basal+0.10% de lisina a partir de PSA, 6 dieta basal+0.05% de lisina a partir de PSB y 7 dieta basal+0.10% de lisina a partir de PSB. Los resultados de crecimiento, se explicaron mediante la ecuación de regresión lineal múltiple, con los datos de ganancia de peso, consumos de lisina sintética o consumos de lisina a partir de las pastas de soya fue Y= 375 .419 + 0.0378 X1 + 0.0366 X2 + 0.0376 X3; en donde X1 correspondió a la complementación con L-lisina, X2 con la PS A (con adición de cascarilla cruda y X3 con la PSB (con adición de cascarilla cocida. Al comparar la pendiente de la pasta de soya A o B, con la obtenida con L-lisina (100%, se tuvieron biodisponibilidades de lisina de 97 y 99 % p ara las pastas de soya A y B respectivamente. Estos resultados indican que la AU de la pasta de soya afecta la biod isponibilidad de lis ina; a mayor actividad ureásica, menor es la disponibilidad.

  6. El uso de granos secos de destilería con solubles (DDGS) en dietas sorgo-soya para pollos de engorda y gallinas de postura

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    Arturo Cortes Cuevas; César A. Esparza Carrillo; Gonzalo Sanabria Elizalde; Jorge Miguel Iriarte; Manuel Ornelas Roac; Ernesto Ávila González

    2012-01-01

    Para estudiar el comportamiento productivo en pollos y gallinas con dietas adicionadass con granos secos de destilería con solubles (DDGS), se realizaron dos experimentos. En el primero se emplearon 704 pollos Ross de 1 a 49 días de edad, distribuidos en cuatro tratamientos con ocho repeticiones de 22 pollos cada uno. Se empleó un diseño completamente al azar. Los tratamientos fueron: 1) dieta basal sorgo-soya, T2) como 1+7% DDGS, T3) como 1+14% de DDGS, T4) como 1+21% de DDGS. En el segundo ...

  7. El uso de granos secos de destilería con solubles (DDGS en dietas sorgo-soya para pollos de engorda y gallinas de postura

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    Arturo Cortes Cuevas

    2012-01-01

    Full Text Available Para estudiar el comportamiento productivo en pollos y gallinas con dietas adicionadass con granos secos de destilería con solubles (DDGS, se realizaron dos experimentos. En el primero se emplearon 704 pollos Ross de 1 a 49 días de edad, distribuidos en cuatro tratamientos con ocho repeticiones de 22 pollos cada uno. Se empleó un diseño completamente al azar. Los tratamientos fueron: 1 dieta basal sorgo-soya, T2 como 1+7% DDGS, T3 como 1+14% de DDGS, T4 como 1+21% de DDGS. En el segundo experimento, se utilizaron 288 gallinas Bovans White de 35 semanas de edad, alojadas en jaulas durante 56 días de experimentación. Se usó un diseño completamente al azar de cuatro tratamientos con tres repeticiones de 12 gallinas cada una. Los tratamientos fueron: 1 dieta basal sorgo-soya, 2 como 1+3% DDGS, 3 como 1+6 % de DDGS, 4 como 1+9% de DDGS. Los resultados del Exp 1, indicaron que la adición del 7% de DDGS, no afectó el comportamiento productivo y de la canal (P>0.05. Los resultados en el Exp 2, mostraron que el empleo de 3, 6 y 9 % de DDGS no afectó el comportamiento productivo (P>0.05. Hubo un efecto benéfico (P<0.05 en la pigmentación de la yema del huevo, siendo mayor en los tratamientos con DDGS. Con los resultados obtenidos en este estudio, se puede concluir que la adición de DDGS en dietas sorgo-soya de hasta el 7 % en pollos de engorda y hasta 9 % en gallinas de postura, es una fuente alternativa de proteína y energía.

  8. Utilización de proteina de soya y carragenina en salchichas tipo Huacho con bajo tenor graso

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    Salva Ruíz, Bettit Karim

    2008-01-01

    Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Tecnología de Alimentos El presente trabajo de investigación consistió en determinar la influencia de la sustitución de grasa por la adición de hidrocoloides (proteina texturizada de soya, carragenina, concentrado funcional de soya), pellejo de cerdo y combinaciones de estas en las características de la salchicha tipo Huacho. Se emplearon concentraciones de 20, 30, 40 y 50% de proteína texturizada de soya, para reduci...

  9. Crecimiento de alevines de Cichlasoma urophthalmus con dietas basadas en diferentes niveles de inclusión de proteína de soya y gluten de trigo

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    Villarreal, C; Gelabert, R; Gaxiola, G; Cuzon, G; Amador, LE; Guevara, E; Brito, R

    2011-01-01

    Se determinó el efecto de seis dietas con diferente contenido de proteína de origen vegetal sobre el crecimiento de alevines de Cichlasoma urophthalmus. Se utilizaron dietas isoenergéticas e isolipídicas con contenido de proteína entre 10% y 60%, empleando soya y gluten de trigo como las principales fuentes proteicas. Los tratamientos no afectaron la supervivencia, la cual fue superior al 80% en todos los casos. Los índices de crecimiento variaron significativamente de acuerdo al contenido pr...

  10. Productividad y eficiencia de uso de nitrógeno y energía en pollos de engorda alimentados con pasta de soya o pasta de canola

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    Sergio Gómez Rosales

    2012-01-01

    Full Text Available Se realizaron dos experimentos de sacrificio para evaluar el crecimiento, contenido y deposición de tejidos y retención de proteína y energía en la carne de la canal en pollos en crecimiento alimentados con pasta de canola (PCAN en sustitución de pasta de soya (PSOY. En el Exp 1, seis pollos machos, de 43 días de edad, se sacrificaron al inicio y 36 pollos fueron asignados a tres dietas con cantidades crecientes de PCAN (0, 10 y 20 % combinadas con dos niveles de lisina digestible (LD: 0.85 y 0.95 %. En el Exp 2, seis hembras y seis machos, de 28 días de edad, se sacrificaron al inicio y 72 pollos fueron asignados, por sexo, a dos dietas (PSOY o PCAN como único ingrediente proteico combinadas con tres niveles de energía (3.0, 3.1 y 3.2 Mcal de EM/kg de alimento. Cada experimento duró dos semanas y al final todos los pollos fueron sacrificados. En el Exp 1, no hubo diferencias estadísticas en la productividad o retención de proteína y energía en la canal entre niveles de PCAN (P>0.05. En el Exp 2, el consumo de alimento, proteína y energía y la deposición de grasa fueron mayores (P<0.05 con PSOY, pero la ganancia de peso, eficiencia alimenticia y retención de proteína y energía en la canal fueron similares entre dietas. Los resultados indican que es factible sustituir parcial o totalmente la pasta de soya por pasta de canola en la dieta de pollos de engorda en crecimiento.

  11. Utilización de harina de yuca y soya integral cocida en dietas con dos formas de presentación para lechones de prelevante

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    Yaruro Maria Liliana

    1988-12-01

    Full Text Available En lechones de prelevante (35 días 20kg se evaluó al reemplazo de 50 y 100% del maíz por harina de yuca en combinación con torta de soya y soya integral cocida (SIC. Presentada en forma de harina y peletizada. En ensayo tuvo un arreglo factorial de 2 x 2 x 2 + 1 ,5 replicaciones. Las unidades experimentales o camadas estuvieron constituidas por lo menos por 6 lechones puros o cruzados de las razas Yorkshire, Landrace y Duroc. No hubo diferencias (P<0.05 en consumo de alimento, aumento de peso, conversión alimenticia y edad para alcanzar 20 kg de peso debidas al reemplazo del maíz en la dieta. Hubo diferencias (P<0.01 en aumento de peso a tribuidas a la fuente de proteína de la dieta, produciendo mejores rendimientos la torta de soya + aceite que el grano cocido. Hubo diferencias (P < 0.01 en el consumo de alimento debidas a la forma de presentación del alimento. Las dietas en harina produjeron mejores rendimientos en consumo de alimento y aumento de peso, mientras que la conversión alimenticia fue mejor en las dietas peletizadas. Económicamente la mejor alternativa constituye la dieta con 100010 harina de yuca + torta de soya.In postweaning pigs (35 days, 20 kg was evaluated the replacement 50 % and 100 % of corn by cassava meal, using soybean meal (SBM and whole coocked soybean in a meal or pelleted diet. The experiment was conducte with a complete random design and a factorial arrangement 2 x 2 x 2 + 1 and 5 replications. Each experimental unit was constitutive for a Iitter with a minimum of 6 piglets, pure and (or crossbred (Yorkshire, Duroc, Landrace. Diets were formulated to meet the nutrient requirements followed by NRC (1979, except energy requirements (3600 Kcal M.E. /kg. Were no differences (P< 0.05 in feed consumption, weight gain, feed conversion and days to reach 20kg live wight in pigs due to the replacement of 50 % and 100 % of corn by cassava meal, there were differences(P<0.05 in weight gain due to the protein source

  12. Cuantificación de polifenoles, flavonoides totales e isoflavonas en arepas elaboradas con adición parcial de harina desgrasada de soya (Glycine max

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    Petra Beatriz Navas H.

    2013-12-01

    Full Text Available La arepa aunque es un producto de consumo masivo en Venezuela, es un alimento con limitaciones desde el punto de vista nutricional. En este sentido, se evaluó el efecto de la incorporación de la harina que se obtiene como subproducto de la extracción mecánica del aceite de soya, sobre el enriquecimiento en componentes bioactivos (polifenoles y flavonoides de una harina de maíz empleada en la elaboración de arepas. Se hicieron arepas utilizando harina de maíz precocida (HMP comercial como control (100 % y mezclas con adición parcial de 7, 10 y 15 %, empleando harina de la torta residual desgrasada de soya (HTRDS. Se efectuaron las siguientes determinaciones analíticas a las harinas: humedad, grasa, almidón, y absorción de agua, asimismo se determinó proteína en las harinas, lisina disponible, polifenoles, flavonoides e isoflavonas (genisteína y daidzeína tanto a las harinas como a las arepas elaboradas, estas últimas fueron evaluadas sensorialmente por un grupo de 12 catadores previamente entrenados en Análisis Descriptivo Cuantitativo y constituido por personas que son consumidores frecuentes de este alimento. Los resultados mostraron que la HTRDS es una fuente importante de componentes bioactivos, con concentraciones de polifenoles y de flavonoides totales de 2079,7 μg ácido cafeico/g y de 1295,2 μg quercetina/g, respectivamente. La incorporación de 7 % de HTRDS produjo arepas con 130,0 μg ácido cafeico/g y 82,0 μg quercetina/g de polifenoles y flavonoides totales, respectivamente; y para las isoflavonas 103,0 μg/kg y 124,7 μg/kg de genisteína y daidzeína, respectivamente. La evaluación sensorial mostró que esta proporción de HTDRS fue la preferida en todos los atributos sensoriales, luego de la muestra control.

  13. Análisis proximal, evaluación microbiológica y sensorial de carnes para hamburguesas elaboradas con cachama blanca (Piaractus brachypomus) y soya (Glycine max) texturizada

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    Oscar García; Iria Acevedo; Jorge Ruiz Ramírez

    2013-01-01

    La cachama blanca (Piaractus brachypomus) es una especie económicamente importante en la acuicultura continental de América Latina y una alternativa nacional de producción de pescado para la piscicultura, la industria y el consumo. El objetivo del presente trabajo de investigación fue caracterizar mediante análisis proximal, evaluación microbiológica y sensorial, carnes para hamburguesas elaboradas con pulpa de cachama y diferentes inclusiones porcentuales de harina de soya texturizada (HST) ...

  14. INOCULACIÓN AL SUELO CON Pseudomonas fluorescens, Azospirillum oryzae, Bacillus subtilis Y MICROORGANISMOS DE MONTAÑA (MM Y SU EFECTO SOBRE UN SISTEMA DE ROTACIÓN SOYA-TOMATE BAJO CONDICIONES DE INVERNADERO

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    Leida Castro Barquero

    2015-01-01

    Full Text Available Se evaluó un sistema de rotación soya- tomate, con incorporación de biomasa verde y aplicación de inóculos microbianos individuales y en mezcla sobre el crecimiento de las plantas y propiedades edáficas; para ello se evaluaron en invernadero por 24 meses los siguientes 9 trata - mientos: solo tomate (T; rotación tomate-soya (TS; rotación tomate-soya con inoculaciones individuales de Azospirillum oryzae (A; de Pseudomonas fluorescens (P; de Bacillus sub - tilis (B; de microorganismos de montaña (MM; y las inoculaciones en mezcla de B. subtilis y P. fluorescens (BP; de B. subtilis, P. fluorescens y A. oryzae ( BPA; d e B. subtilis , P. fluorescens , Azospirillum sp. y MM (BPAMM. Se evalua - ron las variables físicas: densidad aparente y de partículas; conductividad hidráulica; poros totales; estabilidad de agregados; resistencia a la penetración (RP; las variables químicas: pH; conductividad eléctrica; contenido de N y C; relación C/N; contenido de nutrientes en suelos y foliares; las variables biológicas: respiración de suelos, unidades formadoras de colonias de hongos, bacterias y actinomicetos y el peso fres - co y seco foliar. Las variables físicas no fueron afectadas por los tratamientos, con excepción de RP, que fue mayor en el tratamiento T. Las varia - bles biológicas y químicas fueron sensibles a los tratamientos, con valores significativamente más altos en presencia de MM. Destaca el incremento del P en solución de suelo en tratamientos a los que se adicionó MM: pasó de 6 a 20 mg.l -1 ; esta condición se reflejó además en la cantidad de P en el tejido foliar al final del ensayo. Se determi - nó que el pH, CE y la respiración del suelo fueron afectados por la interacción entre los tratamientos aplicados y el tiempo transcurrido; los mayores valores se obtuvieron al final del ensayo y en los tratamientos con MM.

  15. Diseño de los procesos para industrializar la soya

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    Barrigas Romero, Cesar Orlando; Miranda, Luis

    2009-01-01

    El objetivo general es el diseño de los procesos y de las líneas de producción a escala semi industrial para la obtención de productos alimenticios a base de soya. Para ello, en la primera parte se describe a la soya como materia prima, en la fase experimental establecimos parámetros de proceso para estandarizar resultados con 1 kilo de soya como base a fin de obtener leche, queso y yogurt de soya con la parte líquida; y con la parte sólida un producto tipo granola y tortitas a base de la mez...

  16. Propiedades fisicoquimicas de hot-dog de carne de llama con inclusión de albúmina, goma de tara y prote´ina de soya - ciia 2017

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    Apaza, C.; Universidad Peruana Unión, Puno Juliaca, Perú; Elias, C.; Universidad Nacional Agraria la Molina, Lima, Perú; Salvá, B.; Universidad Nacional Agraria la Molina, Lima, Perú

    2017-01-01

    Se evaluó el perfil de textura (TPA), Aw y pH de hot-dog elaborado a base a carne de llama; se trabajó con 16 formulaciones que incluyeron diferentes niveles de inclusión de albúmina de huevo (AH), goma de tara (GT) y concentrado funcional de soya (CFS). Mediante el TPA se evaluó la cohesividad, masticabilidad, dureza, gomosidad y elasticidad. Para evaluar el efecto individual y sinérgico de los componentes se utilizó el método de Diseño de mezclas. Se encontró que la adición de CFS influenci...

  17. Propiedades fisicoquimicas de hot-dog de carne de llama con inclusión de albúmina, goma de tara y proteína de soya - CIIA 2017

    OpenAIRE

    Apaza, C.; Elias, C.; Salvá, B.

    2017-01-01

    RESUMEN Se evaluó el perfil de textura (TPA), Aw y pH de hot-dog elaborado a base a carne de llama; se trabajó con 16 formulaciones que incluyeron diferentes niveles de inclusión de albúmina de huevo (AH), goma de tara (GT) y concentrado funcional de soya (CFS). Mediante el TPA se evaluó la cohesividad, masticabilidad, dureza, gomosidad y elasticidad. Para evaluar el efecto individual y sinérgico de los componentes se utilizó el método de Diseño de mezclas. Se encontró que la adición de CFS i...

  18. Inoculación al suelo con Pseudomonas fluorescens, Azospirillum oryzae, Bacillus subtilis y microorganismos de montaña (mm y su efecto sobre un sistema de rotación soya-tomate bajo condiciones de invernadero

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    Leida Castro Barquero

    2015-11-01

    Full Text Available Se evaluó un sistema de rotación soyatomate, con incorporación de biomasa verde y aplicación de inóculos microbianos individuales y en mezcla sobre el crecimiento de las plantas y propiedades edáficas; para ello se evaluaron en invernadero por 24 meses los siguientes 9 tratamientos: solo tomate (T; rotación tomate-soya (TS; rotación tomate-soya con inoculaciones individuales de Azospirillum oryzae (A; de Pseudomonas fluorescens (P; de Bacillus subtilis (B; de microorganismos de montaña (MM; y las inoculaciones en mezcla de B. subtilis y P. fluorescens (BP; de B. subtilis, P. fluorescens y A. oryzae (BPA; de B. subtilis, P. fluorescens, Azospirillum sp. y MM (BPAMM. Se evaluaron las variables físicas: densidad aparente y de partículas; conductividad hidráulica; poros totales; estabilidad de agregados; resistencia a la penetración (RP; las variables químicas: pH; conductividad eléctrica; contenido de N y C; relación C/N; contenido de nutrientes en suelos y foliares; las variables biológicas: respiración de suelos, unidades formadoras de colonias de hongos, bacterias y actinomicetos y el peso fresco y seco foliar. Las variables físicas no fueron afectadas por los tratamientos, con excepción de RP, que fue mayor en el tratamiento T. Las variables biológicas y químicas fueron sensibles a los tratamientos, con valores significativamente más altos en presencia de MM. Destaca el incremento del P en solución de suelo en tratamientos a los que se adicionó MM: pasó de 6 a 20 mg.l-1; esta condición se reflejó además en la cantidad de P en el tejido foliar al final del ensayo. Se determinó que el pH, CE y la respiración del suelo fueron afectados por la interacción entre los tratamientos aplicados y el tiempo transcurrido; los mayores valores se obtuvieron al final del ensayo y en los tratamientos con MM.

  19. Análisis proximal, evaluación microbiológica y sensorial de carnes para hamburguesas elaboradas con cachama blanca (Piaractus brachypomus y soya (Glycine max texturizada

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    Oscar García

    2013-12-01

    Full Text Available La cachama blanca (Piaractus brachypomus es una especie económicamente importante en la acuicultura continental de América Latina y una alternativa nacional de producción de pescado para la piscicultura, la industria y el consumo. El objetivo del presente trabajo de investigación fue caracterizar mediante análisis proximal, evaluación microbiológica y sensorial, carnes para hamburguesas elaboradas con pulpa de cachama y diferentes inclusiones porcentuales de harina de soya texturizada (HST (0, 3, 6 y 9 %. Se realizó análisis proximal a las carnes crudas y cocidas, se evaluó microbiológicamente a las crudas y sensorialmente las cocidas con 100 consumidores. En las carnes para hamburguesas a mayor adición de HST favoreció la retención de agua durante la cocción y se elevó el contenido de proteína, grasa y cenizas en las carnes crudas y cocidas (p < 0,05. El análisis microbiológico reveló inocuidad alimentaria en las carnes para hamburguesas crudas, encontrándose todos los valores por debajo de lo establecido en la norma venezolana COVENIN 2127-1998 para hamburguesa y otras normas de referencia. La blandura aumentó de manera proporcional al incremento porcentual en la inclusión de HST y las formulaciones con 0, 3 y 6 % de HST se diferenciaron significativamente (p < 0,05 de la formulación con 9 %. La apariencia de las carnes de hamburguesa agradó más en las formulaciones 6 y 9 %, la blandura en 9 %, y el sabor en el control (0 %, seguido de 3 %. Algunos consumidores hicieron asociaciones de sabor a carne de pollo, mariscos y hervidos de pollo.

  20. Estudio de Factibilidad para la creación de una empresa productora y comercializadora de leche de Soya en el Sectro Norte de Quito

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    Andrango Logacho, Geovanna Inés

    2009-01-01

    La semilla de soya ha formado parte de la dieta de los seres humanos por casi 5.000 años y a diferencia de la mayoría de los alimentos provenientes de las plantas, la semilla de soya es rica en proteínas y se considera equivalente a los alimentos provenientes de animales en términos de calidad de la proteína que la soya contiene. La soya es un cultivo con efectos beneficiosos para los suelos, es una oleaginosa de alto valor nutritivo y de múltiples usos en el procesamiento ...

  1. Efectos de la soya en la mucosa endometrial de mujeres posmenopáusicas

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    Delia Jara

    2006-06-01

    Full Text Available Objetivo: Describir los cambios en la morfología del endometrio y expresión de receptores a estrógenos, en mujeres posmenopáusicas que reciben soya vía oral. Diseño: Estudio descriptvo, observacional, de tipo longitudinal y prospectivo. Lugar: Instituto de Patología, Universidad Nacional Mayor de San Marcos. Pacientes: Veintidós mujeres posmenopáusicas tratadas con soya. Intervenciones: A mujeres posmenopáusicas, tratadas con 60 g de soya por día, durante 6 meses, se les realizó biopsia por aspiración del endometrio, antes y después del tratamiento, efectuando estudios histológico e inmunohistoquímico. Principales medidas de resultados: Modificaciones histológicas del endometrio y de expresión de receptores de estrógenos y progesterona. Resultados: El rango de edad de las pacientes estuvo entre 46 y 65 años, con promedio de 53 años. Hubo aparente mejoría en los síntomas climatéricos vasomotores. El endometrio antes del tratamiento con soya en 19 pacientes (86% era atrófico y en 3 de ellas (14%, inactivo. Luego del tratamiento con soya, 17 (77,3% persistieron como endometrio atrófico, 4 (18,2% mostraron endometrio inactivo y el endometrio de una paciente mostró cambios proliferativos. La expresión de receptores para estrógenos, antes del tratamiento con soya, en 19 pacientes (86% fue negativo; después del tratamiento 16 (76% permanecieron negativos. Conclusiones: Después del tratamiento con soya, en mujeres posmenopáusicas, durante 6 meses, se observó persistencia de endometrio atrófico y se mantuvo la expresión negativa para receptores hormonales de estrógenos.

  2. Inhibición de los contaminantes del tempeh de soya

    OpenAIRE

    Mejías, E.; Delgado, R.; Silveira, I.; Lafargue, D.; Cárdenas, E.

    2008-01-01

    Se evaluó el efecto de diferentes especias o condimentos sobre la calidad microbiológica del tempeh y la posible acción inhibidora de sus contaminantes. El tempeh se obtuvo a partir de frijol de soya el cual se sometió a los procesos de cocción, escurrimiento y fermentación. A continuación se procedió a incorporar los condimentos ensayados en dos proporciones por separado a la soya fresca antes de su inoculación con la cepa R. oligosporus, se mezclaron bien y se someti...

  3. Danube Soya – Improving European GM-free soya supply for food and feed

    Directory of Open Access Journals (Sweden)

    Krön Matthias

    2015-09-01

    Full Text Available The Danube Soya Association is an international, voluntary, non-profit, non-governmental association promoting sustainable and GMO**-free soya production along the Danube River Basin – from Switzerland to the Black Sea. Founded in 2012 the association counts today more than 200 members from the whole value chain and civil society in 16 European countries. The members share the vision of a protein transition towards more European and more sustainable protein sources for food and feed. The Danube Area is an area of large potential agricultural surpluses and Danube Soya promotes the idea of using these surpluses to replace a part of the imported soya. The goals of the association are to increase value-added in the rural economies of Central Eastern Europe, promote European cooperation as well as a production of constant and sustainable local soya sources. The Danube Soya Association runs a certification scheme that reaches from soya seeds all the way to final products, which can be labelled with the consumer label “Fed with Danube Soya” (for animal products and “Danube Soya” (for soya food products. Together with its partners, the Danube Soya Association implements and supports dissemination and research projects on different topics.

  4. Synthesis of epoxidised soya bean oil acrylate

    International Nuclear Information System (INIS)

    Hussin bin Mohd Nor; Mohamad Hilmi bin Mahmood; Dahlan bin Haji Mohd.

    1988-10-01

    An epoxy acrylate was synthesized from Asahi's epoxy resin AER 331 which is an epoxidised soya bean oil (ESBO). Triethylamine (TEA) and Hydroquinone (HQ) were used as catalyst and inhibitor respectively. Observations of the experiment are described. (author)

  5. Development and mapping of a public reference set of SSR markers in Lolium perenne L.

    NARCIS (Netherlands)

    Bach, J.L.; Muylle, H.; Arens, P.F.P.; Andersen, C.H.; Bach Holm, P.; Ghesquiere, M.; Julier, B.; Lubberstedt, T.; Nielsen, K.K.; Riek, de J.; Roldán-Ruiz, I.; Roulund, N.; Taylor, C.; Vosman, B.J.; Barre, P.

    2005-01-01

    We report on the characterization and mapping of 76 simple sequence repeat (SSR) markers for Lolium perenne. These markers are publicly available or obtained either from genomic libraries enriched for SSR motifs or L. perenne expressed sequence tag (EST) clones. Four L. perenne mapping populations

  6. Quality, safety, biofunctionality and fermentation control in soya

    NARCIS (Netherlands)

    Nout, M.J.R.

    2015-01-01

    Fermented soya foods play an important role in the diet and health of a large part of the world population. We will discuss the manufacture and properties of: soya sauce; semi-solid pastes such as miso, doujiang and doenjang; and solid fermented soya foods such as natto, kinema, tempe, sufu and

  7. Effects of fermented soya bean on digestion, absorption and diarrhoea

    NARCIS (Netherlands)

    Kiers, J.L.

    2001-01-01

    For many centuries Asian people have consumed soya beans in various forms of traditional fermented soya bean foods. Major desirable aspects of fermented soya bean foods are their attractive flavour and texture, certain nutritional properties, and possible health promoting effects. This

  8. Properties of soya milk and tofu prepared with gamma-irradiated soya beans

    International Nuclear Information System (INIS)

    Byun, M.W.; Kang, I.J.

    1995-01-01

    Soya beans (Glycine max) 'hwang keum' were gamma-irradiated at dose levels of 0, 2 5, 5, 10 and 20 kGy and the effects of the irradiated soya beans on soya milk and tofu properties were studied. An irradiation dose of 5 kGy caused an increase in yield of soya milk and tofu while having very little effect on their quality. The properties of tofu prepared with the soya beans irradiated at 2.5-5 kGy showed no significant difference from the non-irradiated control. However, at higher doses (10-20 kGy), decreases in yield, water holding capacity and sag value of tofu were observed. Compared with the non-irradiated control, hardness and fracturability in the texture of tofu were both significantly increased when the soya bean had been irradiated at 10-20 kGy, while cohesiveness and adhesiveness decreased. The changes in color values of soya milk and tofu were pronounced at 20 kGy

  9. ELISA for Detection of Soya Proteins in Meat Products

    Directory of Open Access Journals (Sweden)

    Eva Renčová

    2009-01-01

    Full Text Available Indirect competitive ELISA method for the detection of soya proteins in meat products was developed. The detection limit of the method is 0.5% of the weight of added soya protein. A total of 131 meat product samples such as salamis or sausages from the Czech Republic market were investigated for the presence of soya proteins. Soya proteins were detected in 84% of the investigated samples without any declaration on the package of the product. The use of vegetable additives, namely soya in meat products in the market of the Czech Republic is very frequent and the restriction of its usage by legislation relates only to some kinds of durable products and ham (Act 264/2003 Coll.. The need for sensitive inspecting methods for soya protein detection is not only associated with the economic aspect (adulteration, but mainly with consumer health protection in case of allergy to soya proteins.

  10. Danube Soya – Improving European GM-free soya supply for food and feed

    OpenAIRE

    Krön Matthias; Bittner Ursula

    2015-01-01

    The Danube Soya Association is an international, voluntary, non-profit, non-governmental association promoting sustainable and GMO**-free soya production along the Danube River Basin – from Switzerland to the Black Sea. Founded in 2012 the association counts today more than 200 members from the whole value chain and civil society in 16 European countries. The members share the vision of a protein transition towards more European...

  11. Effects of soya fatty acids on cassava ethanol fermentation.

    Science.gov (United States)

    Xiao, Dongguang; Wu, Shuai; Zhu, Xudong; Chen, Yefu; Guo, Xuewu

    2010-01-01

    Ethanol tolerance is a key trait of microbes in bioethanol production. Previous studies have shown that soya flour contributed to the increase of ethanol tolerance of yeast cells. In this paper, the mechanism of this ethanol tolerance improvement was investigated in cassava ethanol fermentation supplemented with soya flour or defatted soya flour, respectively. Experiment results showed that ethanol tolerance of cells from soya flour supplemented medium increased by 4-6% (v/v) than the control with defatted soya flour. Microscopic observation found that soya flour can retain the cell shape while dramatic elongations of cells were observed with the defatted soya flour supplemented medium. Unsaturated fatty acids (UFAs) compositions of cell membrane were analyzed and the UFAs amounts increased significantly in all tested strains grown in soya flour supplemented medium. Growth study also showed that soya flour stimulated the cell growth rate by approximately tenfolds at 72-h fermentation. All these results suggested that soya fatty acids play an important role to protect yeast cells from ethanol stress during fermentation process.

  12. Elaboración de un sucedáneo de café (Coffea arabica L. a base de soya (Glycine max L.

    Directory of Open Access Journals (Sweden)

    María Carolina Otálora Rodríguez

    2010-12-01

    Full Text Available El presente trabajo consistió en la preparación, caracterización fisicoquímica, microbiológica y sensorial de un sucedáneo de café empleando soya (variedad Soyica P-34. Se establecieron tres tipos de tostión (tostión baja 211 ºC - 550 s, media 216 ºC - 600 s; y alta 222 ºC - 640 s. Se preparó una bebida y se realizó análisis sensorial cualitativo del color aroma y sabor, y evaluación sensorial cuantitativa descriptiva de ocho características. La soya tostada y molida presentó disminución en los contenidos de proteína bruta, lípidos totales y carbohidratos en relación a la soya sin tostar (2,42 %; 3,78 % y 5,24 %, respectivamente. El tamaño de partícula del polvo de soya tostado y molido tuvo un promedio de 0,5 mm de diámetro medio, correspondiente a una molienda media. No hubo diferencia significativa entre la bebida preparada con el polvo de granos de soya obtenido de la tostión media (216 ºC - 600 s y el patrón (p > 0,05. Se presentaron claros indicios que la bebida sustituta de café en base a soya tostada que presentó mayor aceptación fue la que se elaboró con polvo de soya obtenido del proceso de tostión media. El producto obtenido representa una alternativa de consumo en sustitución del café tradicional, con valor nutricional, agradables atributos sensoriales y beneficios para la salud.

  13. Producción de biomasa y costos de producción de pastos estrella africana (Cynodon nlemfuensis, kikuyo (Kikuyuocloa clandestina y ryegrass perenne (Lolium perenne en lecherías de Costa Rica

    Directory of Open Access Journals (Sweden)

    Luis Villalobos

    2013-01-01

    Full Text Available Se evaluó la producción de biomasa, los costos de producción y el costo del kilogramo de materia seca en los pastos kikuyo (Kikuyuocloa clandestina, ryegrass perenne (Lolium perenne y estrella africana (Cynodon nlemfuensis, a lo largo de un año, en 8 fincas comerciales ubicadas en las provincias de Cartago (2, San José (2 y Alajuela (4. La producción de biomasa promedio por ciclo para los 3 pastos fue de 3395 kg.ha-1 MS; la producción anual se ve influenciada por los días de recuperación de cada especie, mostrando valores de 40 170, 38 731 y 28 995 kg.ha-1 de MS para los pastos estrella africana, kikuyo y ryegrass perenne, respectivamente. La producción de biomasa varía durante el año y en las épocas de mayor producción de esa biomasa, los animales tienen un menor aprovechamiento de la pastura en términos porcentuales, debido a que la carga animal, los períodos de permanencia y las áreas de pastoreo no se ajustan a la disponibilidad de forraje. Los costos anuales de mano de obra, insumos y tierra promedio fueron de 72.433, 505.515 y 18.760 colones.ha-1, respectiva- mente; siendo los insumos el rubro con un peso relativo mayor en la estructura de costos de las fincas en pastoreo. Los costos del kg de MS producido y consumido, para los 3 pastos evaluados, fueron de 16,6 y 44,4 colones respectivamente, siendo el aprovechamiento que los animales hacen de las pasturas el determinante del costo del material consumido. Las fincas con mayor inversión anual en pasturas, mostraron un mayor retorno en kg.ha-1 leche.

  14. Estudio del contenido de ácidos grasos superiores en veinte variedades tropicales de soya

    Directory of Open Access Journals (Sweden)

    Boris Kocelj Ramírez

    2009-05-01

    Full Text Available Muy poco se sabe acerca del contenido de ácidos grasos en las diferentes variedades tropicales de soya. En este trabajo se estudiaron veinte variedades tropicales de soya en lo referente a su contenido de aceite y en ácidos grasos. Se determinaros las propiedades físicas y químicas del aceite que lo caracteizan como comestible. El contenido cualitativo y cuantitativo de ácidos grasos individuales se investigó utilizando la técnica de cromatografía de gases. Se analizaron los éteres correspondientes, obtenidos por esterificación catalítica completa con trifluoruro de Boro-metanol. Se encontraron diferencias significativas entre las propiedades de los aceites de las variedades estudiadas. Se identificaron los ácidos: palmítico, esteárico, linoleico y linolénico

  15. Flowering does not decrease vegetative competitiveness of Lolium perenne

    DEFF Research Database (Denmark)

    Thiele, Jan; Bagger Jørgensen, Rikke; Hauser, Thure Pavlo

    2009-01-01

    as reduced flowering could free resources and increase productivity. But if so, less-flowering cultivars might be more competitive and invade natural swards. We tested for costs of sexual reproduction on vegetative propagation and competitiveness of the perennial grass Lolium perenne, one of the most...... treatments were successful in producing clones with largely differing degrees of flowering. However, we found no negative correlation between flowering and vegetative propagation and competitiveness. Early and strongly flowering southern provenances showed less clonal growth and higher mortality, but within...... provenances the response of clone diameter to flowering was positive or neutral. We conclude that investment of resources into flowering has no measurable costs on vegetative propagation and competitiveness of L. perenne. The apparent lack of costs of sexual reproduction could be explained by bet...

  16. Estrategia reproductiva de una hierba perenne: Hypoxis decumbens (Hypoxidaceae

    Directory of Open Access Journals (Sweden)

    Elena Raimúndez U

    1998-09-01

    Full Text Available Se estudió la estrategia reproductiva de Hypoxis decumbens L. (Hypoxidaceae, una hierba perenne con flores hermafroditas, para comprobar las relaciones que se establecen entre sistema reproductivo, forma de vida, ambiente, sistema de polinización y otros caracteres morfológicos y ecológicos. H. decumbens es una especie altamente autógama, que presenta depresión por entrecruzamiento debido a la adaptación de los genotipos autógamos a su ambiente. Los principales polinizadores fueron insectos generalistas como los dípteros. Presentó flores funcionalmente solitarias, actinomórficas, con las partes florales bien expuestas, todas a la misma altura, y anteras de dehiscencia introrsa. La asignación de biomasa fue básicamente hacia estructuras femeninas, como frutos y semillas, lo que se reflejó en una alta eficiencia reproductiva. La relación polen/óvulo fue baja, lo mismo que la relación biomasa de atracción/biomasa de soporte. La unidad de dispersión fue la semilla y el síndrome de dispersión asociado fue la mirmecocoría. En esta especie se presenta un sistema reproductivo mixto, y aunque la mayor proporción de progenie es autógama, no sacrifica la oportunidad de entrecruzarse.The reproductive strategy of Hypoxis decumbens L. (Hypoxidaceae, a perennial herb with hermaphroditic flowers, was studied to check the relations between reproductive system, life form, habitat, pollination system and other morphological and ecological features. The study was done in a secondary deciduous forest with a population of 150-200 plants. Controlled pollinations were done in twenty isolated plants. The results pointed out that H. decumbens is an autogamous species, with outbreeding depression apparently reflecting environmental adaptation of the autogamous genotypes. The main pollinators were generalist insects, like dipterans. It has functionally solitary and actinomorphic flowers, with well exposed floral parts, displayed approximately at the

  17. Herbage Production, Nutritive Value and Grazing Preference of Diploid and Tetraploid Perennial Ryegrass Cultivars (Lolium perenne L. Producción de Fitomasa, Calidad Nutritiva y Preferencia de Pastoreo de Cultivares Diploides y Tetraploides de Ballica Perenne (Lolium perenne L.

    Directory of Open Access Journals (Sweden)

    Oscar A Balocchi

    2009-09-01

    Full Text Available The objective of this study was to determine, under the soil and climatic conditions of Southern Chile, the effect of the ploidy of perennial ryegrass (Lolium perenne L. cultivars on herbage production, nutritive value, grazing preference and utilization of pasture produced. This study was conducted in southern Chile, Valdivia Province, and was evaluated for 3 years. The tetraploid cultivars used were Quartet (4n, Gwendal (4n, Pastoral (4n and Napoleon (4n. The diploid cultivars were Anita (2n, Jumbo (2n, Aries (2n, and Yatsyn 1 (2n.When the average sward height reached 20 cm, all plots were simultaneously grazed by dairy cows for a period of 24 h. Before and after grazing, sward height, dry matter availability and nutritive value were evaluated. Grazing preference was visually assessed every 5 min for a period of 2.5 h after the afternoon milking. During the 3-year period 20 grazing events were evaluated. A randomized complete block design, with eight cultivars and three replicates, was used. Diploid cultivars showed greater herbage mass accumulation than tetraploid cultivars (P ≤ 0.05. No significant differences were obtained in the annual average crude protein content. Nevertheless, tetraploid cultivars showed a greater D value than diploid cultivars, except during the third year when the difference was not statistically significant. Dairy cows grazed more time on tetraploid cultivars. Considering, additionally, the residual herbage mass after grazing and the percentage of pasture utilization, diploid cultivars were less intensively grazed, suggesting a lower consumption by the cows.El objetivo de este estudio fue determinar, bajo las condiciones edafoclimáticas del sur de Chile, el efecto de la ploidía de cultivares de ballica perenne (Lolium perenne L. sobre el rendimiento de fitomasa, calidad nutricional, preferencia de pastoreo y porcentaje de utilización del forraje producido. El ensayo se realizó en el sur de Chile, provincia de

  18. Factores asociados con la pérdida de germinación y vigor en la semilla de dos cultivares de soya Glycine max (L. en postcosecha Factors associated with low vigor and loss of germination of two soybean varieties in post harvest

    Directory of Open Access Journals (Sweden)

    Agudelo D. Orlando

    1990-12-01

    Full Text Available La investigación tuvo como finalidad evaluar algunos factores que inciden en la pérdida de germinación y vigor de la semilla de soya, tales como los contenidos de ácidos grasos libres y el índice de peróxidos, el tiempo de almacenamiento y el grosor de la cubierta seminal. También se pretendió evaluar los efectos de la profundidad de siembra (3 y 6 cm y textura del substrato de germinación (arena y suelo. La fase experimental se desarrolló en 1990 en ICA Palmira, Las semillas de los cultivares SOYICAP. 31 Y SOYICA P.33 permanecieron almacenados por espacio de noventa días en condiciones ambientales. Se hicieron evaluaciones mensuales de germinación, vigor, contenido de humedad, contenido de ácidos grasos libres y contenido de peróxidos; además se realizó un análisis del grosor de la cubierta seminal y de los cotiledones de cada cultivar. La más alta calidad fisiológica de la semilla se obtuvo a los noventa días de almacenamiento. El contenido de ácidos grasos libres y peróxidos afectó la capacidad del almacenamiento de ésta a través del tiempo. El mayor porcentaje de germinación e índice de vigor en cada variedad coincidió con menores valores tanto en el contenido de ácidos grasos como en el índice de peróxidos. El substrato suelo y la profundidad de siembra de seis centímetros, estuvieron asociados con el menor porcentaje de germinación e índice de vigor. La mejor expresión del vigor y germinación se obtuvo en el substrato arena y la profundidad de siembra de tres centímetros. Se encontró que la variedad SOYICA P. 33 presenta un menor grosor de la testa que la SOYICA P. 31. No existen diferencias sobresalientes, ni morfológicamente ni en cuanto a medidas en las estructuras cotiledonales de las dos variedades.In the National Center of Agricultural Research (ICA of Palmira and Facultad de Ciencias Agropecuarias of Palmira a experirnent with the objetive to see the loss of germination and low vigor in seed

  19. Granjas avícolas y autosuficiencia de maíz y soya: caso Tungurahua-Ecuador

    Directory of Open Access Journals (Sweden)

    Pablo Pomboza-Tamaquiza

    2018-01-01

    Full Text Available Objetivo: caracterizar las granjas avícolas y la autosuficiencia de maíz y soya, en Tungurahua-Ecuador. Metodología: se realizaron entrevistas a noventa avicultores seleccionados mediante muestreo no probabilístico; se utilizó estadísticas de producción y exportaciones reportadas por la Asociación de Fabricantes de Balanceados (AFABA y el Ministerio de Agricultura, Ganadería, Acuacultura y Pesca (MAGAP, y se calcularon índices de Tasa Media de Variación Acumulada y autosuficiencia y dependencia alimentaria. Resultados: en Tungurahua el 78% de avicultores se dedican a la crianza de aves de postura y el 62% tienen instalaciones semiautomatizadas. El 100% de maíz y soya utilizado por la avicultura de Tungurahua, procede de otras provincias e importaciones internacionales. Entre el 2007 y 2016, el maíz registró un índice de autosuficiencia promedio anual de 0.9, mientras que la soya registró 0.08. Limitaciones: la información estadística de producción nacional e importaciones de granos se encontró dispersa. Conclusiones: los resultados indican la presencia de diversos tipos de sistemas de producción avícola. Éstos, según el nivel tecnológico, van desde los artesanales hasta los automatizados. Así mismo, la producción nacional alcanza a cubrir con dificultad la demanda nacional del sector avícola, no así la soya nacional que cubre una mínima parte. Con base en éstos resultados se sugiere mantener la política de sustitución de importaciones de maíz amarillo y ampliar a la soya.

  20. Effect of soya bean diet preparations on some haematological and ...

    African Journals Online (AJOL)

    Effects of Soya bean diet preparations on the hematocrit, hemoglobin concentration, total plasma protein, plasma albumin, sodium, potassium and chloride concentrations were studied in male albino rats. The animals were fed diets containing 75%, 50% and 25% Soya bean in groups II, III and IV respectively. Group I rats ...

  1. Effect of fermented soya beans on diarrhoea and feed efficiency in weaned piglets

    NARCIS (Netherlands)

    Kiers, J.L.; Meijer, J.C.; Nout, M.J.R.; Rombouts, F.M.; Nabuurs, M.J.A.; Meulen, van der J.

    2003-01-01

    Aims: To evaluate anti-diarrhoeal and growth enhancing properties of fermented soya beans in weaned piglets. Methods and Results: In a first phase piglet diet, toasted full-fat soya beans (20%) were replaced with either cooked soya beans or Rhizopus microsporus or Bacillus subtilis fermented soya

  2. Modeling of hydrogenation reactor of soya oil

    International Nuclear Information System (INIS)

    Sotudeh-Gharebagh, R.; Niknam, L.; Mostoufi, N.

    2008-01-01

    In this paper, a batch hydrogenation reactor performance was modeled using a hydrodynamic and reaction sub-models. The reaction expressions were obtained from the information reported in literature. Experimental studies were conducted in order to generate the experimental data needed to validate the model. The comparison between the experimental data and model predictions seems quite satisfactory considering the hydrodynamic limitations and simplifications made on the reaction scheme. The results of this study could be considered as framework in developing new process equipment and also soya oil product design for new applications

  3. Sistemas de siembra para soya de invierno en Veracruz, México

    Directory of Open Access Journals (Sweden)

    Oscar H. Tosquy Valle

    2006-01-01

    Full Text Available La finalidad de este trabajo fue determinar en el norte del estado de Veracruz, México, si la tecnología generada sobre sistemas de siembra de soya en temporal, puede adaptarse a las condiciones de riego. El experimento se estableció en Medellín de Bravo, Veracruz, México, mediante el diseño de bloques al azar con cuatro repeticiones en factorial 2 x 3 x 2 y arreglo de surcos en franjas. Se evaluaron dos distancias entre surcos: 60 y 75 cm, tres densidades: 150.000, 250.000 y 350.000 plantas/ha y dos genotipos: la línea DM 301 y la variedad Huasteca 200. Aunque con el surcado a 60 cm se obtuvo una mayor altura y número de entrenudos por planta, el rendimiento fue similar con ambos distanciamientos. Con 150.000 plantas/ha se produjeron más vainas por planta, pero con densidades mayores, las plantas fueron más altas, con más entrenudos y tuvieron un rendimiento promedio superior en 571,7 kg/ha. Huasteca 200 tuvo un rendimiento de 3.268 kg/ha, superior en 21,8% al obtenido por DM 301. Se concluye que existe potencial para producir soya de riego en invierno en el centro de Veracruz. La mayor producción se obtuvo con la variedad Huasteca 200 a 250.000 plantas/ha, en surcos espaciados a 60 ó 75 cm

  4. [Soya isoflavones and evidences on cardiovascular protection].

    Science.gov (United States)

    González Cañete, Natalia; Durán Agüero, Samuel

    2014-06-01

    Soya isoflavones represent a group of non-nutritive, bioactive compounds, of non-steroidal phenolic nature that are present in soy bean and derived foods. They share with other compounds the capacity of binding to estrogenic receptors from different cells and tissues so that they may act as phytoestrogens. The current interest in these compounds comes from the knowledge that in Asian populations with high levels of their consumption the prevalence of cancer and cardiovascular disease is lower, as compared to the Western countries populations. This cardiovascular benefit would be the result not only of the modulation of plasma lipids, which is a widely studied mechanism. This paper reviews the published evidence about the beneficial effects of soya isoflavones and the different mechanisms of action that would benefit cardiovascular health and that surpass the mechanisms traditionally approached such as the modulation of plasma lipids, and that implicate the regulation of cellular and enzymatic functions in situations such as inflammation, thrombosis, and atherosclerotic progression. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  5. Inflammatory markers in a randomised soya intervention among men.

    Science.gov (United States)

    Maskarinec, Gertraud; Oum, Robert; Chaptman, Ann K; Ognjanovic, Simona

    2009-06-01

    The present analysis investigated the effect of soya foods on serum levels of six inflammatory markers, leptin, adiponectin, monocyte attractant protein 1 (MCP-1), macrophage inflammatory protein-1b (MIP-1b), IL-6 and C-reactive protein (CRP), and their relationship with BMI and lifetime soya intake. We randomised twenty-four men to a high- (two daily servings with 30-35 mg isoflavones per serving) or a low-soya diet for 3 months. After a 1-month washout period, the men crossed over to the other treatment. We used a multiplex bead immunoassay to measure leptin, adiponectin, MCP-1 and MIP-1b and ELISA assays for IL-6 and CRP. The statistical analysis applied mixed models that incorporated the four repeated measurements. The men had a mean age of 58.7 (sd 7.2) years and a mean BMI of 28.4 (sd 4.9) kg/m2. We observed no significant intervention effect of the soya treatment on any of the six markers. After adjustment for age and ethnicity, highly significant associations of BMI and body weight with leptin and MCP-1 emerged. Men with high soya intake early in life also had higher levels of leptin and MCP-1, whereas no association was seen for soya intake during adulthood. MIP-1b, adiponectin, IL-6 and CRP were not related to BMI, body weight or soya intake at any time in life. No intervention effect of soya foods on markers of inflammation was observed in this small study, but adiposity and early-life soya intake were related to higher leptin and MCP-1 levels.

  6. Producción y valor nutricional del forraje de soya en condiciones tropicales adversas

    Directory of Open Access Journals (Sweden)

    Carlos Tobía

    2004-01-01

    Full Text Available Una parcela de soya (Glycine max L. Merr. var. ‘CIGRAS-06’ de 2,4 ha, cultivada en condiciones de alta precipitación y temperatura y en un suelo ácido (pH 4,78 con baja fertilidad, en La Virgen de Sarapiquí, produjo un promedio de 4,8 t ha-1 de materia seca, cuando la planta entera se cosechó para forraje en el estado de desarrollo R6 (semillas llenas. Esta productividad es baja, en comparación con la obtenida en Guanacaste y San Carlos, donde las condiciones de clima y suelo fueron favorables para la producción de esta especie. La composición química de la planta entera fue de 20,2% de proteína, 6,7% de grasa, 5,5% de cenizas, 42,2% de fibra detergente neutro y 25,4% de carbohidratos no fibrosos. La proporción del peso seco de las hojas: vainas+semillas:tallos+pecíolos de las plantas fue de 24:39:37, respectivamente. Un análisis económico de los resultados señala que la producción de forraje y de ensilaje tal como fue ofrecido tuvo un costo de 0,02 y 0,03 US $ kg-1, respectivamente. Se pone en evidencia que aún en las condiciones adversas de clima y suelo donde tuvo lugar esta investigación, es posible aprovechar las cualidades nutricionales de la soya en la alimentación del ganado lechero, a un costo relativamente bajo y sin los inconvenientes y la alta inversión en maquinaria y equipo que representa la producción de soya para semilla en el trópico húmedo.

  7. Bioestadística en cultivos Perennes Biostatistics in Perennial crops

    Directory of Open Access Journals (Sweden)

    Martínez B. Ricardo

    1989-12-01

    Full Text Available Por definición, los cultivos perennes viven más tiempo y crecen más que los cultivos anuales; por lo tanto, en los experimentos con ellos se tropieza con problemas especiales. Los experimentos con frecuencia duran varios años, así que las plantas están sujetas a riesgos por más largo tiempo, aumentando la posibilidad de accidentes y así generar datos desbalanceados. La naturaleza tiene más oportunidades de eventos inconvenientes como inundaciones o caídas de plantas por el viento. Un trabajador descuidado tiene más chance de dañar una planta con un tractor o de aplicar un tratamiento en la parcela equivocada. El diseño experimental,
    por lo tanto, debe poder tolerar el hecho inevitable de los datos faltantes. Los objetivos experimentales pueden
    cambiar con el tiempo, requiriendo modificaciones en el plan original. Además, al concluir un experimento se puede tener interés en llevar a cabo un nuevo ensayo sobre las
    mismas plantas. Por lo tanto, es conveniente contar con diseños experimentales que sean flexibles. Diseños experimentales sencillos llenan, por lo general, los requisitos de los perennes; los diseños complejos son inflexibles
    y se presentan problemas computacionales y de interpretación cuando hay datos faltantes. En los experimentos con perennes grandes probablemente se usarán parcelas con una sola planta o con pocas plantas. Proporcionalmente, la variablidad de parcela a parcela se deberá menos a la variación debido a efectos ambientales y más a la variación de planta a planta comparado con los cultivos anuales. El intento de reducir la varianza del error
    experimental con solo aumentar el número de replicaciones dentro de un diseño estándar puede no ser satisfactorio. Se ha encontrado que el análisis de covarianza es muy efectivo para reducir la varianza del error, esa efectividad va desde 25 hasta 50%. Los datos muy seguramente incluirán
    varias observaciones recolectadas en

  8. THE EFFECT OF REPLACING SOYA BEAN MEAL WITH COOKED ...

    African Journals Online (AJOL)

    2016-01-01

    Jan 1, 2016 ... performance, carcass characteristics and blood indices of finisher broilers. Mucuna sloanei seeds were ... that is rich in protein and used for both human and ... The over dependence on soya bean as major protein source for ...

  9. Utilization of soya protein as an alternative protein source in ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-01-05

    Jan 5, 2009 ... For carcass trait, ash, crude fat, and energy varied significantly with soya protein ... high-protein content, relatively well-balanced amino acid profile ..... and organoleptic quality of flesh of brook char (Salvelinus fontinalis).

  10. Improving oxidative stability of soya and sunflower oil using ...

    African Journals Online (AJOL)

    Rosmarinus officinallis) and tert- butyl-hydroquinone (TBHQ) as possible antioxidants in sunflower and soya oil. Upon addition of 200 ppm of dried leaf extract, acetone extract yielded protection of the samples against oxidation more efficiently as ...

  11. Preparation and characterization of soaps made from soya bean oil ...

    African Journals Online (AJOL)

    This research work deals with the preparation of soaps from neem oil and soya bean oil blends and analyses the soap produced. The soaps were produced using cold process technique by varying the percentage of oils; (soya bean oil and neem oil) in the ratio of 100%, 90/10%, 80/20%, 70/30%, 60/40%, 50/50%, 40/60%, ...

  12. Quantifying Dustiness, Specific Allergens, and Endotoxin in Bulk Soya Imports

    Directory of Open Access Journals (Sweden)

    Howard J. Mason

    2017-11-01

    Full Text Available Soya is an important bulk agricultural product often transported by sea as chipped beans and/or the bean husks after pelletisation. There are proven allergens in both forms. Bulk handling of soya imports can generate air pollution containing dust, allergens, and pyrogens, posing health risks to dockside workers and surrounding populations. Using an International Organization for Standardization (ISO standardised rotating drum dustiness test in seven imported soya bulks, we compared the generated levels of dust and two major soya allergens in three particle sizes related to respiratory health. Extractable levels of allergen and endotoxin from the bulks showed 30–60 fold differences, with levels of one allergen (hydrophobic seed protein and endotoxin higher in husk. The generated levels of dust and allergens in the three particle sizes also showed very wide variations between bulks, with aerolysed levels of allergen influenced by both the inherent dustiness and the extractable allergen in each bulk. Percentage allergen aerolysed from pelletized husk—often assumed to be of low dustiness—after transportation was not lower than that from chipped beans. Thus, not all soya bulks pose the same inhalation health risk and reinforces the importance of controlling dust generation from handling all soya bulk to as low as reasonably practicable.

  13. Watershed responses to Amazon soya bean cropland expansion and intensification.

    Science.gov (United States)

    Neill, Christopher; Coe, Michael T; Riskin, Shelby H; Krusche, Alex V; Elsenbeer, Helmut; Macedo, Marcia N; McHorney, Richard; Lefebvre, Paul; Davidson, Eric A; Scheffler, Raphael; Figueira, Adelaine Michela e Silva; Porder, Stephen; Deegan, Linda A

    2013-06-05

    The expansion and intensification of soya bean agriculture in southeastern Amazonia can alter watershed hydrology and biogeochemistry by changing the land cover, water balance and nutrient inputs. Several new insights on the responses of watershed hydrology and biogeochemistry to deforestation in Mato Grosso have emerged from recent intensive field campaigns in this region. Because of reduced evapotranspiration, total water export increases threefold to fourfold in soya bean watersheds compared with forest. However, the deep and highly permeable soils on the broad plateaus on which much of the soya bean cultivation has expanded buffer small soya bean watersheds against increased stormflows. Concentrations of nitrate and phosphate do not differ between forest or soya bean watersheds because fixation of phosphorus fertilizer by iron and aluminium oxides and anion exchange of nitrate in deep soils restrict nutrient movement. Despite resistance to biogeochemical change, streams in soya bean watersheds have higher temperatures caused by impoundments and reduction of bordering riparian forest. In larger rivers, increased water flow, current velocities and sediment flux following deforestation can reshape stream morphology, suggesting that cumulative impacts of deforestation in small watersheds will occur at larger scales.

  14. Implementation of genomic prediction in Lolium perenne (L. breeding populations

    Directory of Open Access Journals (Sweden)

    Nastasiya F Grinberg

    2016-02-01

    Full Text Available Perennial ryegrass (Lolium perenne L. is one of the most widely grown forage grasses in temperate agriculture. In order to maintain and increase its usage as forage in livestock agriculture, there is a continued need for improvement in biomass yield, quality, disease resistance and seed yield. Genetic gain for traits such as biomass yield has been relatively modest. This has been attributed to its long breeding cycle, and the necessity to use population based breeding methods. Thanks to recent advances in genotyping techniques there is increasing interest in genomic selection from which genomically estimated breeding values (GEBV are derived. In this paper we compare the classical RRBLUP model with state-of-the-art machine learning (ML techniques that should yield themselves easily to use in GS and demonstrate their application to predicting quantitative traits in a breeding population of L. perenne. Prediction accuracies varied from 0 to 0.59 depending on trait, prediction model and composition of the training population. The BLUP model produced the highest prediction accuracies for most traits and training populations. Forage quality traits had the highest accuracies compared to yield related traits. There appeared to be no clear pattern to the effect of the training population composition on the prediction accuracies. The heritability of the forage quality traits was generally higher than for the yield related traits, and could partly explain the difference in accuracy. Some population structure was evident in the breeding populations, and probably contributed to the varying effects of training population on the predictions. The average linkage disequilibrium (LD between adjacent markers ranged from 0.121 to 0.215. Higher marker density and larger training population closely related with the test population are likely to improve the prediction accuracy.

  15. Soya Fasulyesinden Konsantre Protein Üretimi ve Soya Ürünlerinin Bileşim Unsurları

    OpenAIRE

    Artık, Nevzat

    2015-01-01

    Soya fasulyesi üretimi ülkemizde 1981 verilerine göre 15000 ton düzeyindedir. Ortalama verim 88 kg da olarak gerçekleşmektedir. Ülkemizde üretilen soya fasulyesinin büyük bir çoğunluğu hayvan yemi olarak kullanılmaktadır. Bileşimi çok zengin olan ve hayvansal proteine eşdeğer oranda protein içeren soya fasulyesi gereğince değerlendirilememektedir. Soya fasulyesinden proteince zengin değişik ürünler elde edilmektedir. Bunlar soya unu, konsantre soya proteini, soya izolatı, et analogu ve ferme...

  16. EL ANÁLISIS BAYESIANO Y LA PRECISIÓN DE LOS VALORES DE LA HEREDABILIDAD EN ESPECIES PERENNES

    Directory of Open Access Journals (Sweden)

    Freddy Mora

    2009-01-01

    Full Text Available Los objetivos del presente trabajo fueron evaluar la precisión del valor estimado de la heredabilidad determinada por medio de la desviación estándar, considerando un enfoque Bayesiano, y comparar tal estimativa con el procedimiento clásico. Se utilizaron datos de un ensayo de progenie con 39 familias de Eucalyptus cladocalyx. La variable dependiente usada fue el diámetro basal del fuste medido a los seis años de edad. El método Bayesiano fue implementado por medio del algoritmo de Cadenas Independientes, con informaciones a priori informativas, el cual entregó bajos valores de desviaciones estándar de la heredabilidad, si comparado con la estimación clásica de Robertson y distribución a priori de Jeffreys (la cual es una clase de distribución a priori no informativa y a menudo impropia. El método de análisis Bayesiano es una herramienta de inferencia valiosa para la evaluación genética de especies perennes, ya que considera la variabilidad de los parámetros por medio de las distribuciones a posteriori.

  17. TRATAMIENTO DE OLORES PROVENIENTES DEL PROCESO DE SAPONIFICACION DE BORRAS DE SOYA

    Directory of Open Access Journals (Sweden)

    Ramiro Escalera Vásquez

    2006-01-01

    Full Text Available Se presenta un estudio de laboratorio sobre el desarrollo de un sistema híbrido de tratamiento de olores provenientes de procesos de saponificación de borras de soya. El sistema consiste en un absorbedor de gases seguido por un tanque contactor entre aceites esenciales nebulizados y los gases remanentes del proceso de absorción. Se caracterizaron física y químicamente varias muestras de las borras de soya obtenidas de proveedores nacionales y los gases generados en la saponificación de esas borras. Se probó la efectividad de tres sistemas de tratamiento diseñados y construidos en laboratorio: Un sistema cerrado; un sistema de contacto entre esencias nebulizadas y los gases malolientes y el sistema híbrido mencionado. Los gases sin tratar tienen como principales componentes compuestos orgánicos volátiles (VOCs soluble y no solubles, amoníaco y dióxido de carbono. Las borras con mayor contenido de proteína residual desprenden mayores cantidades de amoníaco libre. Se  establecieron relaciones confiables entre las dosis de aceites esenciales nebulizados y la concentración de VOCs y el Umbral de Olor Mínimo Detectable (CUOMD. El producto de cabeza del aceite de eucalipto es la esencia más efectiva y económica para el tratamiento de los olores generados por los tres tipos de borra de soya probados. Se eliminó totalmente la percepción de olores desagradables utilizando el sistema híbrido que demostró ser el más eficiente. Los métodos y aparatos experimentales utilizados pueden servir para investigaciones similares en problemas de olor que actualmente tienen diversas industrias locales y nacionales.

  18. The fate of phosphorus fertilizer in Amazon soya bean fields.

    Science.gov (United States)

    Riskin, Shelby H; Porder, Stephen; Neill, Christopher; Figueira, Adelaine Michela e Silva; Tubbesing, Carmen; Mahowald, Natalie

    2013-06-05

    Fertilizer-intensive soya bean agriculture has recently expanded in southeastern Amazonia, and whereas intensive fertilizer use in the temperate zone has led to widespread eutrophication of freshwater ecosystems, the effects in tropical systems are less well understood. We examined the fate of fertilizer phosphorus (P) by comparing P forms and budgets across a chronosequence of soya bean fields (converted to soya beans between 2003 and 2008) and forests on an 800 km(2) soya bean farm in Mato Grosso, Brazil. Soya bean fields were fertilized with 50 kg P ha(-1) yr(-1) (30 kg P ha(-1) yr(-1) above what is removed in crops). We used modified Hedley fractionation to quantify soil P pools and found increases in less-plant-available inorganic pools and decreases in organic pools in agricultural soils compared with forest. Fertilizer P did not move below 20 cm. Measurements of P sorption capacity suggest that while fertilizer inputs quench close to half of the sorption capacity of fast-reacting pools, most added P is bound in more slowly reacting pools. Our data suggest that this agricultural system currently has a low risk of P losses to waterways and that long time-scales are required to reach critical soil thresholds that would allow continued high yields with reduced fertilizer inputs.

  19. Grain product of 34 soya mutant lines

    International Nuclear Information System (INIS)

    Salmeron E, J.; Mastache L, A. A.; Valencia E, F.; Diaz V, G. E.; Cervantes S, T.; De la Cruz T, E.; Garcia A, J. M.; Falcon B, T.; Gatica T, M. A.

    2009-01-01

    This work was development with the objective of obtaining information of the agronomic behavior of 34 soya mutant lines (R 4 M 18 ) for human consumption and this way to select the 2 better lines. The genetic materials were obtained starting from the variety ISAAEG-B M2 by means of the application of recurrent radiation with Co 60 gammas, to a dose of 350 Gray for the first two generations and both later to 200 Gray and selection during 17 cycles, being obtained the 34 better lines mutants with agronomic characteristic wanted and good flavor. The obtained results were that the mutant lines L 25 and L 32 produced the major quantity in branches/plant number with 7.5 and 7.25, pods/plant number with 171.25 and 167, grains/plant number with 350.89 and 333.07 and grain product (ton/ha) to 15% of humidity 5.15 and 4.68 ton/ha, respectively. (Author)

  20. Producción de soya (Glycine max L. bajo tres sistemas de labranza

    Directory of Open Access Journals (Sweden)

    Rojas Palacio Hernán

    1987-06-01

    Full Text Available

    The goals of this consisted in economically analyzing the conventional pattern with regard to minimum tillage and non tillage; evaluating the influence of the tillage systems on the physical properties of the soil and on the soybean's growth and yield. The experiment was stablished on clay soil. The experimental design employed was that of hierarchy-arranged random blocks with three repetitions (9 experimental units. The conventional tillage permitts the improvement of the physical conditions of the soil, performs a better control on the weeds, gives the soybean plant a full growth and yield causing the highest profitability. The second treatment showing the best efficiency was the minimum tillage.

    Se evaluó la influencia de los sistemas de labranza convencional, mínima y no labranza sobre las propiedades físicas del suelo, en el desarrollo y producción de la soya. El experimento se estableció en suelo arcilloso. El diseño experimental fue el de bloques al azar jerarquizados con tres repeticiones (9 unidades experimentales. La labranza convencional mejora las condiciones físicas del suelo, ejerce mayor control sobre las malezas, confiere a la planta de soya apropiado desarrollo y producción ocasionando así la mayor rentabilidad. El segundo tratamiento en eficiencia fue el de la labranza mínima.

  1. Morus alba o Hibiscus rosa-sinensis como sustituto parcial de soya en dietas integrales para conejos

    Directory of Open Access Journals (Sweden)

    P. E. Lara

    2012-01-01

    Full Text Available Los conejos pueden aprovechar los nutrientes contenidos en los forrajes y subproductos agrícolas eficientemente gracias a la fermentación cecal y a la cecotrofía. Los objetivos del presente trabajo fueron: evaluar la tasa de crecimiento y el rendimiento de la canal en conejos alimentados con minibloques de harina de morera (Morus alba o tulipán (Hibiscus rosa-sinensis en sustitución parcial de pasta de soya; y determinar el consumo y digestibilidad de la dieta. Para esto, se utilizaron 18 conejos de 30 días de edad, distribuidos al azar en tres dietas experimentales (n=6, ac (alimento convencional, mbm (minibloque con 30% de morera y, mbt (minibloque con 27% de tulipán, durante nueve semanas. La ganancia diaria y el rendimiento en canal fue mejor (p0.10 en los tres grupos. Es factible sustituir parcialmente la proteína de la soya por harina de hojas de morera o tulipán, aunque presentan una menor respuesta productiva con respecto a la alimentación convencional.

  2. Efecto de la frecuencia de corte en especies patrenses. (II) Variación del contenido en nitrógeno, fósforo, potasio y azufre de Lolium perenne "V. 807" y Lolium italicum “Tetrone”

    OpenAIRE

    García Criado, Balbino; Duque Macías, Francisco; García Ciudad, Antonia

    2011-01-01

    Se estudia el efecto conjunto de la época de recolección y frecuencia de corte sobre el contenido en nitrógeno, fósforo, potasio y azufre de Lolium perenne V. 807 y Lolium italicum Tetrone. Los cortes se realizan a intervalos de diez, quince, veinte y treinta días en el período de abril a noviembre a 5-10 cm. del suelo. Se considera también la evolución de los elementos con la madurez en cortes mensuales. Los contenidos medios de nitrógeno y fósforo en Lolium perenne y de nitrógeno y azufre e...

  3. A proteomic study to identify soya allergens--the human response to transgenic versus non-transgenic soya samples.

    Science.gov (United States)

    Batista, Rita; Martins, Isabel; Jeno, Paul; Ricardo, Cândido Pinto; Oliveira, Maria Margarida

    2007-01-01

    In spite of being among the main foods responsible for allergic reactions worldwide, soybean (Glycine max)-derived products continue to be increasingly widespread in a variety of food products due to their well-documented health benefits. Soybean also continues to be one of the elected target crops for genetic modification. The aim of this study was to characterize the soya proteome and, specifically, IgE-reactive proteins as well as to compare the IgE response in soya-allergic individuals to genetically modified Roundup Ready soya versus its non-transgenic control. We performed two-dimensional gel electrophoresis of protein extracts from a 5% genetically modified Roundup Ready flour sample and its non-transgenic control followed by Western blotting with plasma from 5 soya-sensitive individuals. We used peptide tandem mass spectrometry to identify soya proteins (55 protein matches), specifically IgE-binding ones, and to evaluate differences between transgenic and non-transgenic samples. We identified 2 new potential soybean allergens--one is maturation associated and seems to be part of the late embryogenesis abundant proteins group and the other is a cysteine proteinase inhibitor. None of the individuals tested reacted differentially to the transgenic versus non-transgenic samples under study. Soybean endogenous allergen expression does not seem to be altered after genetic modification. Proteomics should be considered a powerful tool for functional characterization of plants and for food safety assessment. Copyright (c) 2007 S. Karger AG, Basel.

  4. Quorum Quenching Bacillus sonorensis Isolated from Soya Sauce Fermentation Brine

    Directory of Open Access Journals (Sweden)

    Kok-Gan Chan

    2012-03-01

    Full Text Available An N-acylhomoserine lactone (AHL-degrading bacterial strain, L62, was isolated from a sample of fermentation brine of Chinese soya sauce by using rich medium agar supplemented with soya sauce (10% v/v. L62, a rod-shaped Gram positive bacterium with amylolytic activity, was phylogentically related to Bacillus sonorensis by 16S ribosomal DNA and rpoB sequence analyses. B. sonorensis L62 efficiently degraded N-3-oxohexanoyl homoserine lactone and N-octanoylhomoserine lactone. However, the aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of AHLs, was not detected in L62, suggesting the presence of a different AHL-degrading gene in L62. To the best of our knowledge, this is the first report of AHL-degrading B. sonorensis from soya sauce liquid state fermentation.

  5. Mechanism of Resistance to Glyphosate in Lolium perenne from Argentina

    Directory of Open Access Journals (Sweden)

    Marcos Yanniccari

    2017-10-01

    Full Text Available In Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.

  6. Türkiye’de Yetiştirilen bazı Soya Varyetelerinden Elde Edilen Soya Sütlerinin Amino Asit Profilinin Belirlenmesi

    OpenAIRE

    Artık, Nevzat

    1989-01-01

    Bu araştırmada Türkiye’de yetiştirilen 5 farklı soya varyetesinden üretilen soya sütlerinin amino asit profilleri incelenmiştir. Ayrıca Japon soya varyetesi amino asit bileşimi ile karşılaştırma yapılmıştır. Soya sütlerinde esansiyel amino asitler yüksek düzeyde bulunmuştur. Esansiyel amino asit miktarı tüm soya varyetelerinde 4.125, 4.922 (mg/g) sınırları içinde değişmektedir. Bu özelliği ile soya sütü insan beslenmesinde önemli bir gıda maddesidir.  

  7. Türkiye’de Yetiştirilen bazı Soya Varyetelerinden Elde Edilen Soya Sütlerinin Amino Asit Profilinin Belirlenmesi

    OpenAIRE

    Nevzat Artık

    2015-01-01

    Bu araştırmada Türkiye’de yetiştirilen 5 farklı soya varyetesinden üretilen soya sütlerinin amino asit profilleri incelenmiştir. Ayrıca Japon soya varyetesi amino asit bileşimi ile karşılaştırma yapılmıştır. Soya sütlerinde esansiyel amino asitler yüksek düzeyde bulunmuştur. Esansiyel amino asit miktarı tüm soya varyetelerinde 4.125, 4.922 (mg/g) sınırları içinde değişmektedir. Bu özelliği ile soya sütü insan beslenmesinde önemli bir gıda maddesidir.

  8. Türkiye’de Yetiştirilen bazı Soya Varyetelerinden Elde Edilen Soya Sütlerinin Amino Asit Profilinin Belirlenmesi

    Directory of Open Access Journals (Sweden)

    Nevzat Artık

    2015-02-01

    Full Text Available Bu araştırmada Türkiye’de yetiştirilen 5 farklı soya varyetesinden üretilen soya sütlerinin amino asit profilleri incelenmiştir. Ayrıca Japon soya varyetesi amino asit bileşimi ile karşılaştırma yapılmıştır. Soya sütlerinde esansiyel amino asitler yüksek düzeyde bulunmuştur. Esansiyel amino asit miktarı tüm soya varyetelerinde 4.125, 4.922 (mg/g sınırları içinde değişmektedir. Bu özelliği ile soya sütü insan beslenmesinde önemli bir gıda maddesidir.

  9. Immune response in mice to ingested soya protein: antibody production, oral tolerance and maternal transfer

    DEFF Research Database (Denmark)

    Christensen, Hanne Risager; Pedersen, Susanne Brix; Frøkiær, Hanne

    2004-01-01

    antibody response in the offspring, bat in this case in the absence of oral tolerance. This indicates that, under certain conditions, factors involved in spontaneous antibody production can be transmitted from mother to offspring. Understanding the immune response to soya protein ingested under healthy...... by ELISA, and to the presence of oral tolerance detected as a suppressed antibody and cell-proliferation response upon immunisation with soya protein. F0 mice generated soya-specific antibodies, while oral tolerance to the same soya proteins was also clearly induced. When F0 dams were transferred to soya...

  10. Complete chloroplast genome sequence of a major allogamous forage species, perennial ryegrass (Lolium perenne L.).

    Science.gov (United States)

    Diekmann, Kerstin; Hodkinson, Trevor R; Wolfe, Kenneth H; van den Bekerom, Rob; Dix, Philip J; Barth, Susanne

    2009-06-01

    Lolium perenne L. (perennial ryegrass) is globally one of the most important forage and grassland crops. We sequenced the chloroplast (cp) genome of Lolium perenne cultivar Cashel. The L. perenne cp genome is 135 282 bp with a typical quadripartite structure. It contains genes for 76 unique proteins, 30 tRNAs and four rRNAs. As in other grasses, the genes accD, ycf1 and ycf2 are absent. The genome is of average size within its subfamily Pooideae and of medium size within the Poaceae. Genome size differences are mainly due to length variations in non-coding regions. However, considerable length differences of 1-27 codons in comparison of L. perenne to other Poaceae and 1-68 codons among all Poaceae were also detected. Within the cp genome of this outcrossing cultivar, 10 insertion/deletion polymorphisms and 40 single nucleotide polymorphisms were detected. Two of the polymorphisms involve tiny inversions within hairpin structures. By comparing the genome sequence with RT-PCR products of transcripts for 33 genes, 31 mRNA editing sites were identified, five of them unique to Lolium. The cp genome sequence of L. perenne is available under Accession number AM777385 at the European Molecular Biology Laboratory, National Center for Biotechnology Information and DNA DataBank of Japan.

  11. Effects of soya oligosaccharides and soya oligopeptides on lipid metabolism in hyperlipidaemic rats.

    Science.gov (United States)

    Xie, Shali; Zhu, Jundong; Zhang, Yanqi; Shi, Kai; Shi, Yuangang; Ma, Xiao

    2012-08-01

    In the present study, we aimed to examine the effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on blood lipid levels, release of vasoactive substances, antioxidant activity and faecal bile acid (FBA) excretion in rats fed a high-fat diet (HFD). Male Sprague-Dawley rats were evenly divided into five groups according to diets as follows: regular diet (control), HFD, HFD enriched with 2 % of SOS (SOS), HFD enriched with 3 % of SOP (SOP) and HFD enriched with 2 % SOS and 3 % SOP (SOSP). The results showed that SOS and SOP significantly reduced plasma total cholesterol, LDL-cholesterol and TAG, whereas HDL-cholesterol concentration was significantly increased. Furthermore, SOS and SOP reduced plasma apoB, apoE and the apoB:apoAI ratio, whereas apoAI was significantly increased. Moreover, SOS and SOP also reduced plasma thromboxane A₂ (TXA₂) and the TXA₂:prostacyclin (PGI₂) ratio, whereas plasma PGI₂ and nitric oxide were significantly increased. In addition, SOS and SOP significantly reduced serum and liver malondialdehyde concentrations and increased FBA excretion. However, we did not observe obvious influences of SOS and SOP on superoxide dismutase activities in the liver of HFD-fed rats. The combination of 2 % SOS and 3 % SOP showed a more marked effect than SOS or SOP alone in improving the lipid profile, release of vasoactive substances and increasing FBA excretion (P < 0.05). In summary, SOS and SOP might help prevent atherosclerosis through improving abnormal blood lipid levels, regulating vasoactive substances and protecting against oxidative stress.

  12. LA DINÁMICA DEL USO DE LA TIERRA EN MATO GROSSO: ASPECTOS ECONÓMICOS DEL CULTIVO DE ARROZ, SOYA Y PASTOS

    Directory of Open Access Journals (Sweden)

    Dilamar Dallemole

    2014-08-01

    Full Text Available El cultivo de arroz siempre estuvo presente en el rol de culturas practicadas en el cerrado brasileño, en especial, en el Mato-grossense. Dado a esa particularidad, éste estudio busca comprender mejor las relaciones existentes entre la producción de arroz con las áreas plantadas de soya, y, propiamente, con la área plantada de arroz en Mato Grosso, en el período de 1979 a 2007. Debido a la gran extensión territorial del estado, el cultivo de arroz fue siendo realizado a lo largo de los años como frontera agrícola, para apertura de nuevas áreas, con variedades adecuadas a tierras, inicialmente, con baja fertilidad. Con el desarrollo tecnológico y la posibilidad de nuevas actividades productivas, esa realidad viene se alterando y la área plantada de arroz disminuyó a lo largo de los años, perdiendo espacio para la soya y pastos. Como instrumento metodológico, se empleó un modelo de regresión múltiple, estimada por el Método de Mínimos Cuadrados Ordinarios (MQO, en que los resultados apuntan las correlaciones existentes entre las categorías mencionadas, lo que permite analizar con más propiedad lo que está ocurriendo con el segmento. Las principales conclusiones obtenidas confirman la influencia positiva de la producción de arroz con relación a suya área plantada, sin embargo, negativa para las áreas plantadas de soya, pastos y arroz en Mato Grosso. Aunque venga perdiendo espacio para soya y pastos, el cultivo de arroz, mismo con menor productividad y calidad inferior del grano, aún es practicado debido a su capacidad de adaptación en nuevas áreas, o mismo, en áreas degradadas

  13. Determinación de la calidad nutricional del forraje de soya (Glycine max L. Merr, previamente conservado mediante ensilaje y henificación

    Directory of Open Access Journals (Sweden)

    Olave B. M. del Pilar

    1987-03-01

    Full Text Available Con miras a la implementación de un paquete tecnológico para la doble producción de soya se realizaron 2 experimentos: ensilaje y henificación del forraje de las variedades 50- yica P-32 y "Forrajera "de Proacol. El ensilaje se realizó en bolsas de plástico grueso y se le adicionaron 3 dosis de ácido fórmico, 3 dosis de formaldehído y las combinaciones resultantes (16 tratamientos que se replica ron 3 veces. La henificación se hizo en fardos, con pesos entre 5 y 10 kg. Aunque se demostró la posibilidad de emplear la henificación y el ensilaje del forraje de soya para su conservación, no se encontraron diferencias entre los aditivos estudiados.To look up the implementation of a technologic package for the soya 's double production we did two experiments: silage and hay of the forrage of the varieties Soyica P-32 and Proacol's "Forrajera". Silage was did in heavy plastic bags and added 3 doses of formic acid, 3 doses of formaldehyde and the final combinations of both additives (16 treatments that was applied 3 times. Hay was did in bales that weighted between 5-10 kg. AI though it demonstrated the possibility to use the hay and silage of soja's forrage for conservation, no differences was found between additives studied on the silage.

  14. Genetic differences in root mass of Lolium perenne varieties under field conditions

    NARCIS (Netherlands)

    Deru, J.G.C.; Schilder, H.; Schoot, van der J.R.; Eekeren, van N.J.M.

    2014-01-01

    Although grasses have dense rooting systems, nutrient uptake and productivity can be increased, and N-leaching reduced, if rooting is further improved. The variation in root mass of 16 varieties of Lolium perenne was studied under field conditions in two experiments on sandy soil in The Netherlands.

  15. Towards in vitro fertilization, gametosomatic cybridization and DNA transfer in perennial ryegrass (Lolium perenne L.)

    NARCIS (Netherlands)

    Maas, van der H.M.

    1994-01-01

    In this thesis, research towards invitro fertilization, gametosomatic cybridization andDNAtransfer in perennial ryegrass ( Loliumperenne L.), the most important forage

  16. Estudio de la transformación del Aceite de Soya usado en ésteres etílicos de ácidos grasos

    OpenAIRE

    Sarracent-López, Anabel; Gandón-Hernández, José

    2016-01-01

    La reutilización de los aceites vegetales en la elaboración de alimentos trae efectos nocivos para la salud y por otro lado se necesita de un complejo tratamiento para poder descartarlos sin que afecten al medio ambiente. Transformarlo en ésteres metílicos o etílicos de ácidos graso y glicerina, mediante la transesterificación con el alcohol correspondiente, puede constituir un procedimiento adecuado para su tratamiento. Se investigó con aceite de soya residual, proveniente de una empresa pro...

  17. Inverse association between soya food consumption and insulin resistance in Japanese adults.

    Science.gov (United States)

    Nakamoto, Mariko; Uemura, Hirokazu; Sakai, Tohru; Katsuura-Kamano, Sakurako; Yamaguchi, Miwa; Hiyoshi, Mineyoshi; Arisawa, Kokichi

    2015-08-01

    The purpose of the present study was to examine the association between soya food consumption and insulin resistance using baseline data of the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study in Tokushima, Japan. This cross-sectional study included 1274 subjects, aged 34-70 years at baseline, living in Tokushima Prefecture between 2008 and 2013. Fasting blood samples were collected and information on lifestyle characteristics including soya food intake and medical history were obtained using a structured self-administered questionnaire. The homeostasis model assessment of insulin resistance (HOMA-IR) was measured and those with HOMA-IR ≥ 2.5 were defined as having insulin resistance. Multiple logistic regression models were used to analyse the association between soya product intake and the prevalence of insulin resistance. Rural communities located in Tokushima Prefecture, Japan, between 2008 and 2013. A total of 1148 adults (565 men and 583 women), aged 34-70 years. The frequency of intake of miso soup, total non-fried soya products and total soya products showed significant inverse dose-response relationships with insulin resistance, after adjustments for potential confounders. When soya product intake was calculated as soya protein and isoflavone, the odds ratios of insulin resistance decreased significantly as the estimated intake of soya protein increased. Furthermore, significant inverse dose-response relationships were observed for total non-fried soya products and total soya products, after adjustment for total vegetable or total fibre consumption. The present results indicate that the intake of soya products and non-fried soya products is associated with reduced insulin resistance in the Japanese population.

  18. Feeding differently processed soya bean. Part 2. An assessment of haematological responses in the chicken.

    Science.gov (United States)

    Aletor, V A; Egberongbe, O

    1992-01-01

    The use of differently processed soya bean as a major source of dietary protein was evaluated in a haematological study using broiler chickens in which groundnut cake (GNC), raw soya bean (RSB), roasted soya bean (RtSB), cooked soya bean (CSB) and soya bean oil cake (SBC) were fed on equi-protein basis. The results showed that: 1. Red blood cell (RBC) count and haemoglobin content of blood significantly (P less than 0.05) increased in chicks fed RSB relative to the other soya bean diets. Feeding differently processed soya bean significantly (P less than 0.05) influenced mean cell haemoglobin (MCH) and mean corpuscular volume (MCV) while the mean corpuscular haemoglobin concentration (MCHC) was not significantly influenced. 2. Both the total white blood cell (WBC) count and the monocytes were significantly (P less than 0.05) influenced by the dietary treatments. Chicks fed processed soya bean generally had higher number of monocytes. 3. Physical properties determined were specific gravity and erythrocyte sedimentation rate. The latter was significantly (P less than 0.05) lower in all the processed soya bean-fed chicks. 4. Minerals determined in blood were Na, K, Ca, Mg, Fe, Cu and P. Of all these, chicks fed RSB had significantly (P less than 0.01) lower levels of blood Mg and marked decrease in Ca.

  19. Examination of the safety of gamma-irradiated soya flour

    International Nuclear Information System (INIS)

    Tencheva, S.; Katsareva, Ts.

    1988-01-01

    Fat-free soya flour was used as model food for demonstrating the safety of food additives decontaminated with ionizing radiation. The food was irradiated with 10 kGy and included in the daily food of line C 57 BL mice in amount of 35%. Important medical-biological tests were performed for evaluating the potential toxicity of irradiated food products. Results are reported on a study of three generations of mice, which had persistently consumed 35% irradiated soya flour. The parameters studied were: body mass, mass of internal organs, number of newborn animals and their body mass after weaning, reproductive capacity in F 1a and hematological parameters. The results were summarized in five tables

  20. Protein determination in soya bean by fast neutron activation analysis

    International Nuclear Information System (INIS)

    Szegedi, S.; Mosbah, D.S.; Varadi, M.; Szaloki, I.

    1988-01-01

    For a non-destructive determination of the protein content in soya bean samples, 14-MeV neutron activation analysis was applied. To check the method, the results obtained by X-ray fluorescence analysis and the Kjeldahl procedure were compared. For pressed pellet samples of about 1 g with 15 min irradiation and 10 min measuring times the accuracy of the protein determination was found to be 15%. (author) 7 refs.; 4 figs.; 3 tabs

  1. Detection of Seed-Borne Fungal pathogens on Soya beans

    International Nuclear Information System (INIS)

    Wanyera, R

    2002-01-01

    Soya beans (Glycine max max L.) are propagated by seed and are vulnerable to devastating seed-borne diseases where the importance of each disease varies greatly. Seed-borne diseases cause significant losses in seed, food production and quality of seed and grain. Studies on seed borne diseases in Kenya have not been given emphasis on very important seed crops among the soya beans. The identification and rejection of the seed crop is mainly based on visual appraisal in the field with little or no laboratory work undertaken. Three methods were used to analyse the health status of fifty two soyabean seed samples collected from the National Plant Breeding Research Centre-Njoro and farmers' fields in Bahati division of Nakuru district. The analysis was carried out in the laboratory. The objective of the analysis was to identify and inventory seed-borne fungal pathogens of soya beans grown in Kenya. The normal blotter, herbicide and germination test methods were used. The tests revealed the presence of several important fungal pathogens on soyabean seed samples. Among the pathogens recorded Phoma sp, phomopsis sp, fusarium sp, Hainesia lyhri and Cercospora kikuchii were frequently recorded on the seed samples. Results of the germination test between paper method showed low germination (0-6.7%) on the normal sedlings in all the test samples. Hainesia lyhri was a new record on the soyabean seeds

  2. Effects of calci soya balance and vitagnus on menopausal symptoms.

    Science.gov (United States)

    Golian Tehrani, Shahnaz; Bazzazian, Shahin; Bakhtiarian, Azam; Ghobadzadeh, Maryam

    2014-10-01

    Menopause is a period of women's lives with changes and symptoms that affect their work, sleep and quality of life. Therefore, it is important to overcome these symptoms. The aim of the present study was to compare the effects of Calci soya balance and Vitagnus on menopausal symptoms. This double-blinded controlled trial study was performed in public health centers of Tehran University of Medical Sciences (2011-2012). Seventy postmenopausal women with menopausal symptoms were randomly divided into two groups of treatments with Vitagnus and Calci soya balance. Data were collected using interviews, answering Cooperman's index questionnaires before four and eight weeks after the treatment. Descriptive and analytic statistics were used for analyzing the data. In both groups, Wilcoxon test showed a significant decrease in the mean of Cooperman's menopausal index as well as after four and eight weeks of treatment (P = 0.000). Mann-Whitney test did not show any significant differences between the two groups, before and after four and eight weeks of treatment. The results showed that both Vitagnus and Calci soya balance were effective on reduction of menopausal symptoms to a similar extent and medical community can administer each of these two drugs based on patients' conditions and costs.

  3. Effect of raw soya bean particle size on productive performance and digestion of dairy cows.

    Science.gov (United States)

    Naves, A B; Freitas Júnior, J E; Barletta, R V; Gandra, J R; Calomeni, G D; Gardinal, R; Takiya, C S; Vendramini, T H A; Mingoti, R D; Rennó, F P

    2016-08-01

    Differing soya bean particle sizes may affect productive performance and ruminal fermentation due to the level of fatty acid (FA) exposure of the cotyledon in soya bean grain and because the protein in small particles is more rapidly degraded than the protein in large particles, which influence ruminal fibre digestion and the amounts of ruminally undegradable nutrients. The objective of this experiment was to investigate the effects of raw soya bean particle size on productive performance, digestion and milk FA profile of dairy cows. Twelve Holstein cows were assigned to three 4 × 4 Latin squares with 21-day periods. At the start of the experiment, cows were 121 days in milk (DIM) and yielded 30.2 kg/day of milk. Cows were fed 4 diets: (i) control diet (CO), without raw soya bean; (ii) whole raw soya bean (WRS); (iii) cracked raw soya bean in Wiley mill 4-mm screen (CS4); and (iv) cracked raw soya bean in Wiley mill 2-mm screen (CS2). The inclusion of soya beans (whole or cracked) was 200 g/kg on dry matter (DM) basis and partially replaced ground corn and soya bean meal. Uncorrected milk yield and composition were not influenced by experimental diets; however, fat-corrected milk (FCM) decreased when cows were fed soya bean treatments. Soya bean diets increased the intake of ether extract (EE) and net energy of lactation (NEL ), and decreased the intake of DM and non-fibre carbohydrate (NFC). Ruminal propionate concentration was lower in cows fed WRS than cows fed CS2 or CS4. Cows fed cracked raw soya bean presented lower nitrogen in faeces than cows fed WRS. The milk of cows fed WRS, CS2 and CS4 presented higher unsaturated FA than cows fed CO. The addition of raw soya bean in cow diets, regardless of the particle size, did not impair uncorrected milk yield and nutrient digestion, and increased the concentration of unsaturated FA in milk. Cows fed cracked raw soya bean presented similar productive performance to cows fed whole raw soya bean. Journal of

  4. Umbral numérico de infección de Phakopsora pachyrhizi Sydow & Sydow en folíolos de soya

    Directory of Open Access Journals (Sweden)

    F. R. Garcés

    2012-01-01

    Full Text Available La roya asiática de la soya es una enfermedad importante tanto en Brasil como en el mundo; aunque han sido realizados muchos trabajos en este patosistema, no se conoce todavía una concentración ideal de inóculo para realizar investigaciones con la mencionada enfermedad en condiciones controladas. En este experimento, conducido en invernadero, se procuró identificar el umbral numérico de infección de Phakopsora pachyrhizi en folíolos de soya. Plantas de soya (cultivar Coodetec 214 rr fueron inoculadas con diferentes concentraciones (5x103, 10x103, 15x103, 20x103, 25x103 y 30x103 de uredosporas.ml-1 suspensos en agua. La intensidad de la enfermedad fue evaluada cuantificando la densidad de lesiones y de uredias en un área determinada de los folíolos centrales. Los datos fueron sometidos al análisis de varianza y de regresión, el mismo que mostró significación estadística p<0,01 y explicaron la respuesta biológica y matemática. Con las ecuaciones obtenidas de número de uredias.cm-2, lesiones. cm-2 y de la severidad estimada, se calculó que el número de uredosporas fue de 556 para formar una uredia.cm-2, 909 para formar una lesión.cm-2 y 2,500 para alcanzar 1% de severidad. A partir de las ecuaciones obtenidas en este trabajo, se puede seleccionar una concentración que resulte en una densidad deseada de lesiones y/o uredias de roya asiática de la soya en experimentos con inoculaciones artificiales. Asimismo, con la ecuación resultante de la relación entre número de uredias y lesiones por centímetro cuadrado, el investigador podrá escoger el método de cuantificación más razonable o confiable a su criterio, para trabajos con este patosistema.

  5. Identification of prenylated pterocarpans and other isoflavonoids in Rhizopus spp. elicited soya bean seedlings by electrospray ionisation mass spectrometry

    NARCIS (Netherlands)

    Simons, R.; Vincken, J.P.; Bohin, M.C.; Kuijpers, T.F.M.; Verbruggen, M.A.; Gruppen, H.

    2011-01-01

    Phytoalexins from soya are mainly characterised as prenylated pterocarpans, the glyceollins. Extracts of non-soaked and soaked soya beans, as well as that of soya seedlings, grown in the presence of Rhizopus microsporus var. oryzae, were screened for the presence of prenylated flavonoids with a

  6. Soya-lecithin in extender improves the freezability and fertility of buffalo (Bubalus bubalis) bull spermatozoa.

    Science.gov (United States)

    Akhter, S; Ansari, M S; Andrabi, S M H; Rakha, B A; Ullah, N; Khalid, M

    2012-10-01

    Egg yolk is routinely used as a cryoprotectant in semen extenders. However, it may contain cryoprotective antagonists, and there are hygienic risks associated with its use. Proteins of plant origin, like soya-lecithin, lack these hazards. The aim of this study was to use soya-lecithin as a cryoprotectant in extender and to investigate its effects on in vitro quality and in vivo fertility of buffalo semen. Semen from three buffalo bulls was frozen in tris-citric extender containing 5.0%, 10% or 15% soya-lecithin or 20% egg yolk. Sperm motility, plasma membrane integrity and viability were assessed post-dilution, pre-freezing and post-thaw. In Post-dilution and pre-freezing, the values for motility, plasma membrane integrity and viability remained higher (p ≤ 0.05) in extenders containing 10% soya-lecithin and control compared with extender containing 5% and 15% soya-lecithin. However, motility, plasma membrane integrity and viability were higher (p lecithin compared with control and extenders containing 5% and 15% soya-lecithin. Semen from two buffalo bulls was frozen in tris-citric extender containing either 10% soya-lecithin or 20% egg yolk. Higher (p lecithin (56%) compared with 20% egg yolk (41.5%). The results suggest that 10% soya-lecithin in extender improves the freezability and fertility of buffalo bull spermatozoa and can be used as an alternate to egg yolk in cryopreservation of buffalo semen. © 2011 Blackwell Verlag GmbH.

  7. Building A NGS Genomic Resource: Towards Molecular Breeding In L. Perenne

    DEFF Research Database (Denmark)

    Ruttink, Tom; Roldán-Ruiz, Isabel; Asp, Torben

    To advance the application of molecular breeding in Lolium perenne, we have generated a sequence resource to facilitate gene discovery and SNP marker development. Illumina GAII transcriptome sequencing was performed on meristem-enriched samples of 14 Lolium genotypes. De novo assemblies for indiv......To advance the application of molecular breeding in Lolium perenne, we have generated a sequence resource to facilitate gene discovery and SNP marker development. Illumina GAII transcriptome sequencing was performed on meristem-enriched samples of 14 Lolium genotypes. De novo assemblies...... of SNP markers in selected candidate genes. In parallel, a germplasm collection of 602 Lolium genotypes was established and is being phenotyped for plant architecture, reproductive characteristics, flowering time, and forage quality traits. We will test through association genetics whether phenotypic...

  8. High levels of whole raw soya beans in dairy cow diets: digestibility and animal performance.

    Science.gov (United States)

    Barletta, R V; Gandra, J R; Freitas Junior, J E; Verdurico, L C; Mingoti, R D; Bettero, V P; Benevento, B C; Vilela, F G; Rennó, F P

    2016-12-01

    The aim of this study was to evaluate the effects of high levels of whole raw soya beans in the diets of lactating cows. Twelve Holstein dairy cows were used, randomized in three 4 ×  4 balanced and contemporary Latin squares and fed the following diets: (i) control (C), without including whole raw soya beans; (ii) 80 g/kg in DM of whole raw soya beans (G80); (iii) 160 g/kg in DM of whole raw soya beans (G160); and (iv) 240 g/kg in DM of whole raw soya beans (G240). There was significant reduction (p beans in dairy cow diets improves the unsaturated fatty acid profile in milk, and the diets (G80 and G160) led to minor alterations in the digestive processes and animal metabolism. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  9. Depletion of carbohydrate reserves limits nitrate uptake during early regrowth in Lolium perenne L.

    Czech Academy of Sciences Publication Activity Database

    Guo, Q.; Turnbull, M.; Song, J.; Roche, J.; Novák, Ondřej; Späth, J.; Jameson, P. E.; Love, J.

    2017-01-01

    Roč. 68, č. 7 (2017), s. 1569-1583 ISSN 0022-0957 R&D Projects: GA ČR(CZ) GA17-06613S Institutional support: RVO:61389030 Keywords : Carbohydrate * Carbon * Cytokinin * Fructan * Lolium perenne * Nitrate transporter (NRT) * Nitrate uptake * Nitrogen * Nitrogen use efficiency (NUE) * Perennial ryegrass Subject RIV: EF - Botanics OBOR OECD: Plant sciences, botany Impact factor: 5.830, year: 2016

  10. Producción de maíz y soya forrajera para ensilaje y venta parcial de la cosecha de elotes o chilotes

    Directory of Open Access Journals (Sweden)

    Carlos Jim\\u00E9nez

    2002-01-01

    Full Text Available Este trabajo investigó el efecto de tres diferentes formas de siembra conjunta de maíz y soya: a asociados (un surco maíz-un surco soya, b alternos (cuatro surcos máiz-cuatro surcos soya y c monocultivo y tres formas de cosecha de chilotes o elotes para venta antes de ensilar: a remoción del 50% de los chilotes (a inicio de floración, b remoción del 50% de los elotes (en estado de leche y c sin cosecha. Cada tratamiento tuvo tres repeticiones. Se utilizó la variedad de maíz blanco localmente adaptado, HS-5G de la casa Cristiani Burkard y la variedad de soya CIGRAS 10 para grano y forraje, desarrollada por el Centro de Investigación en Granos y Semillas de la Universidad de Costa Rica y probada con éxito en la regi ón. La cosecha para ensilaje, se realizó en los estados fenol ógicos de inicio de indentación en el maíz y R6 en la soya (semilla formada de consistencia semidura. La remoción del 50% del chilote resultó en $164,06 de ingreso bruto, cubriendo el 44,23 % de los gastos de cultivo, cosecha y almacenamiento en un silo de montón. Sin embargo, se observó una reducci ón desde 5,99 a 4,77 t MS /ha, representando un 20,3 %. La cosecha de elotes también redujo los rendimientos en un 24,4 %, pero permitió recuperar un 89,45 % de los gastos, que equivalieron a $331,75 de ingreso. Al mover los elotes, recupera casi todos los costos, no obstante, hay una reducción en tonelaje y casi de seguro en materia seca digestible. Bajo mejores condiciones de clima, un ingreso bruto mayor sería posible con el consecuente superavit monetario y el ensilaje gratis.

  11. Soya and isoflavone intakes associated with reduced risk of oesophageal cancer in north-west China.

    Science.gov (United States)

    Tang, Li; Lee, Andy H; Xu, Fenglian; Zhang, Taotao; Lei, Jun; Binns, Colin W

    2015-01-01

    To ascertain the association between soya consumption, isoflavone intakes and oesophageal cancer risk in remote north-west China, where the incidence of oesophageal cancer is known to be high. Case-control study. Information on habitual consumption of soya foods and soya milk was obtained by personal interview. The intakes of isoflavones were then estimated using the US Department of Agriculture nutrient database. Logistic regression analyses were performed to assess the association between soya consumption, isoflavone intakes and oesophageal cancer risk. Urumqi and Shihezi, Xinjiang Uyghur Autonomous Region, China. Participants were 359 incident oesophageal cancer patients and 380 hospital-based controls. The oesophageal cancer patients consumed significantly less (P soya foods (mean 57·2 (sd 119·0) g/d) and soya milk (mean 18·8 (sd 51·7) ml/d) than the controls (mean 93·3 (sd 121·5) g/d and mean 35·7 (sd 73·0) ml/d). Logistic regression analyses showed an inverse association between intake of soya products and the risk of oesophageal cancer. The adjusted odds were OR = 0·33 (95 % CI 0·22, 0·49) and OR = 0·48 (95 % CI 0·31, 0·74) for consuming at least 97 g of soya foods and 60 ml of soya milk daily (the highest tertiles of consumption), respectively, relative to the lowest tertiles of consumption. Similarly, inverse associations with apparent dose-response relationships were found between isoflavone intakes and oesophageal cancer risk. Habitual consumption of soya products appears to be associated with reduced risk of oesophageal cancer in north-west China.

  12. Effects of Modifiers on Physiological Metabolism of Lolium perenne Seedlings in Diesel-Polluted Soils

    Directory of Open Access Journals (Sweden)

    ZHAO Xuan

    2017-06-01

    Full Text Available The pot experiment for single-factor with diesel oil polluted soil and the pot experiment for three-factor orthogonal with sawdust-ammonium nitrate-monopotassium phosphate under diesel oil polluted soil with salt stress, were performed to analyze the activity of antioxidant enzymes and chlorophyll content in Lolium perenne seedlings, and to explore the physiological response of L. perenne seedlings under diesel oil polluted soil and its regulations. The results showed that, soil diesel pollution significantly decreased the biomass. Compared with control, activity of superoxide dismutases(SOD in leaf decreased significantly at 0.3% and 0.9% soil diesel pollution, peroxidases (POD and catalase(CAT in leaf decreased significantly at 0.6% and 0.9% soil diesel pollution, the root SOD activity increased significantly at 0.9% diesel concentration while the root POD activity decreased significantly at 0.6% and 0.9% soil diesel pollution. As for the salinity soil polluted by diesel oil, the activity of POD and CAT in leaf increased significantly at 10% volume fraction of sawdust, and the content of chlorophyll a and chlorophyll b increased significantly as well. Meanwhile, chlorophyll a and chlorophyll b content increased significantly at 0.3 g·kg-1 amount of ammonium nitrate. Thereby, sawdust and ammonium nitrate addition could effectively improve physiological metabolic of L. perenne seedlings.

  13. Isolated soya protein with standardised levels of isoflavones, cotyledon soya fibres and soya phospholipids improves plasma lipids in hypercholesterolaemia: a double-blind, placebo-controlled trial of a yoghurt formulation.

    Science.gov (United States)

    Puska, Pekka; Korpelainen, Vesa; Høie, Lars H; Skovlund, Eva; Smerud, Knut T

    2004-03-01

    The objective was to study whether a yoghurt containing isolated soya protein with standardised levels of isoflavones, cotyledon soya fibres and soya phospholipids is more effective in lowering total and LDL-cholesterol than a placebo. One hundred and forty-three subjects were randomised to the soya group (n 69) or to the placebo (n 74). The mean baseline levels were 7.6 and 5.1 mmol/l for total and LDL-cholesterol, respectively. Fasting serum lipoproteins were assessed five times during the 8-week intervention period, and 4 weeks thereafter. The results were analysed by a mixed model for unbalanced repeated measurements. During the intervention, there were highly significant differences in lipid-lowering effect in favour of the active soya intervention group compared with the control group. The significant differences were for total cholesterol (estimated mean difference 0.40 mmol/l; P<0.001), LDL-cholesterol (0.39 mmol/l; P<0.001), non-HDL-cholesterol (0.40 mmol/l; P<0.001) and for the total:HDL-cholesterol ratio (0.23; P=0.005). There was no difference in the effects on HDL-cholesterol, triacylglycerols or homocysteine. The lipid-lowering effect occurred within 1-2 weeks of intervention, and was not due to weight loss. The safety profile for active soya was similar to the placebo group, except for gastrointestinal symptoms, which caused a significantly higher dropout rate (fourteen v. three subjects) among the subjects taking active soya.

  14. New chloroplast microsatellite markers suitable for assessing genetic diversity of Lolium perenne and other related grass species.

    Science.gov (United States)

    Diekmann, Kerstin; Hodkinson, Trevor R; Barth, Susanne

    2012-11-01

    Lolium perenne (perennial ryegrass) is the most important forage grass species of temperate regions. We have previously released the chloroplast genome sequence of L. perenne 'Cashel'. Here nine chloroplast microsatellite markers are published, which were designed based on knowledge about genetically variable regions within the L. perenne chloroplast genome. These markers were successfully used for characterizing the genetic diversity in Lolium and different grass species. Chloroplast genomes of 14 Poaceae taxa were screened for mononucleotide microsatellite repeat regions and primers designed for their amplification from nine loci. The potential of these markers to assess genetic diversity was evaluated on a set of 16 Irish and 15 European L. perenne ecotypes, nine L. perenne cultivars, other Lolium taxa and other grass species. All analysed Poaceae chloroplast genomes contained more than 200 mononucleotide repeats (chloroplast simple sequence repeats, cpSSRs) of at least 7 bp in length, concentrated mainly in the large single copy region of the genome. Nucleotide composition varied considerably among subfamilies (with Pooideae biased towards poly A repeats). The nine new markers distinguish L. perenne from all non-Lolium taxa. TeaCpSSR28 was able to distinguish between all Lolium species and Lolium multiflorum due to an elongation of an A(8) mononucleotide repeat in L. multiflorum. TeaCpSSR31 detected a considerable degree of microsatellite length variation and single nucleotide polymorphism. TeaCpSSR27 revealed variation within some L. perenne accessions due to a 44-bp indel and was hence readily detected by simple agarose gel electrophoresis. Smaller insertion/deletion events or single nucleotide polymorphisms detected by these new markers could be visualized by polyacrylamide gel electrophoresis or DNA sequencing, respectively. The new markers are a valuable tool for plant breeding companies, seed testing agencies and the wider scientific community due to

  15. Evaluación agronómica de germoplasma de soya (Glycine max L. Merr. En Cuba

    Directory of Open Access Journals (Sweden)

    Zoila Fundora

    2003-01-01

    Full Text Available La caracterización y evaluación de las colecciones de soya de que se dispone en Cuba, es uno de los pasos iniciales en la potenciación del germoplasma de esta especie con vistas a su utilización en programas de desarrollo y/o mejoramiento. En el presente trabajo se estudiaron 50 cultivares procedentes de la colección cubana de esta especie, los cuales fueron clasificados preliminarmente, considerando los atributos de mayor variabilidad. Los cultivares fueron agrupados como precoces, intermedios y tardíos, de acuerdo a los días a floración. Se destacan los cultivares más promisorios en el grupo de los precoces y en el grupo de los intermedios + los tardíos respectivamente, recomendándose para su inclusión en los programas de mejoramiento de esta especie.

  16. Towards the development of a sustainable soya bean-based feedstock for aquaculture.

    Science.gov (United States)

    Park, Hyunwoo; Weier, Steven; Razvi, Fareha; Peña, Pamela A; Sims, Neil A; Lowell, Jennica; Hungate, Cory; Kissinger, Karma; Key, Gavin; Fraser, Paul; Napier, Johnathan A; Cahoon, Edgar B; Clemente, Tom E

    2017-02-01

    Soya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. The former is the primary vegetable oil consumed in the world. Hence, its primary usage is in direct human consumption. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean-based protein and a high Omega-3 fatty acid soya bean oil, enriched with alpha-linolenic and stearidonic acids, in both steelhead trout (Oncorhynchus mykiss) and Kampachi (Seriola rivoliana). Communicated herein are aquafeed formulations with major reduction in marine ingredients that translates to more total Omega-3 fatty acids in harvested flesh. Building off of these findings, subsequent efforts were directed towards a genetic strategy that would translate to a prototype design of an optimal identity-preserved soya bean-based feedstock for aquaculture, whereby a multigene stack approach for the targeted synthesis of two value-added output traits, eicosapentaenoic acid and the ketocarotenoid, astaxanthin, were introduced into the crop. To this end, the systematic introduction of seven transgenic cassettes into soya bean, and the molecular and phenotypic evaluation of the derived novel events are described. © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  17. EVALUACIÓN DEL POTENCIAL FORRAJERO DE DIEZ CULTIVARES DE SOYA (Glycine max (L Merr EN VENEZUELA

    Directory of Open Access Journals (Sweden)

    Alexander Hernández

    2013-01-01

    Full Text Available Con el objetivo de evaluar y seleccionar cultivares de soya (Glycine max. (L Merr con fines de forraje, se llevó a cabo un experimento de un diseño en bloques completamente al azar con 6 repeticiones. Los materiales experimentales usados fueron 9 cultivares procedentes del banco de germoplasma de La Fundación DANAC y el cultivar CIGRAS-06 provenientes del Centro de Investigaciones de Granos y Semillas de la UCR. Las variables bajo estudio fueron las relacionadas con la arquitectura de la planta, producción de biomasa total en el estado reproductivo R6, rendimiento de grano y algunas variables bromatológicas. Los análisis estadísticos mostraron diferencias significativas para las variables objeto de estudio. Los cultivares con mayores rendimientos de biomasa fueron CIGRAS-06, FP93-1935, FP93-1924 y FP90-6102 con 17245, 13075, 13066 y 12587 kg.ha-1 de materia seca (MS. Los de mayores contenidos de proteína cruda (PC en las vainas fueron FP90-10205, FP93-1930 y FP92-1904, con 24,2, 23,7 y 21,0%, respectivamente; y a nivel de las hojas los cultivares FP93-1935, FP92-4906 y FP90-7102 con 25,5, 24,9 y 23,0%, respectivamente. Para la producción de grano mostraron alto potencial los cultivares CIGRAS-06, FP93-1924, FP90-6102 y FP90-6103, al superar los 3500 kg.ha-1.

  18. The effects of arbuscular mycorrhizal fungi and root interaction on the competition between Trifolium repens and Lolium perenne.

    Science.gov (United States)

    Ren, Haiyan; Gao, Tao; Hu, Jian; Yang, Gaowen

    2017-01-01

    Understanding the factors that alter competitive interactions and coexistence between plants is a key issue in ecological research. A pot experiment was conducted to test the effects of root interaction and arbuscular mycorrhizal fungi (AMF) inoculation on the interspecies competition between Trifolium repens and Lolium perenne under different proportions of mixed sowing by the combination treatment of two levels of AMF inoculation (inoculation and non-inoculation) and two levels of root interaction (root interaction and non-root interaction). Overall, the aboveground and belowground biomass of T. repens and L. perenne were not altered by AMF inoculation across planting ratios, probably because the fertile soil reduced the positive effect of AMF on plant growth. Both inter- and intraspecies root interaction significantly decreased the aboveground biomass of T. repens , but tended to increase the aboveground biomass of L. perenne across planting ratios, and thus peaked at the 4:4 polyculture. These results showed that T. repens competed poorly with L. perenne because of inter and intraspecies root interaction. Our results indicate that interspecies root interaction regulates the competitive ability of grass L. perenne and legume T. repens in mixtures and further makes great contribution for overyielding. Furthermore, AMF may not be involved in plant-plant interaction in fertile condition.

  19. The effects of arbuscular mycorrhizal fungi and root interaction on the competition between Trifolium repens and Lolium perenne

    Directory of Open Access Journals (Sweden)

    Haiyan Ren

    2017-12-01

    Full Text Available Understanding the factors that alter competitive interactions and coexistence between plants is a key issue in ecological research. A pot experiment was conducted to test the effects of root interaction and arbuscular mycorrhizal fungi (AMF inoculation on the interspecies competition between Trifolium repens and Lolium perenne under different proportions of mixed sowing by the combination treatment of two levels of AMF inoculation (inoculation and non-inoculation and two levels of root interaction (root interaction and non-root interaction. Overall, the aboveground and belowground biomass of T. repens and L. perenne were not altered by AMF inoculation across planting ratios, probably because the fertile soil reduced the positive effect of AMF on plant growth. Both inter- and intraspecies root interaction significantly decreased the aboveground biomass of T. repens, but tended to increase the aboveground biomass of L. perenne across planting ratios, and thus peaked at the 4:4 polyculture. These results showed that T. repens competed poorly with L. perenne because of inter and intraspecies root interaction. Our results indicate that interspecies root interaction regulates the competitive ability of grass L. perenne and legume T. repens in mixtures and further makes great contribution for overyielding. Furthermore, AMF may not be involved in plant–plant interaction in fertile condition.

  20. Soya food intake and risk of endometrial cancer among Chinese women in Shanghai: population based case-control study

    Science.gov (United States)

    Xu, Wang Hong; Zheng, Wei; Xiang, Yong Bing; Ruan, Zhi Xian; Cheng, Jia Rong; Dai, Qi; Gao, Yu Tang; Shu, Xiao Ou

    2004-01-01

    Objective To evaluate the association of intake of soya food, a rich source of phytoestrogens, with the risk of endometrial cancer. Design Population based case-control study, with detailed information on usual soya food intake over the past five years collected by face to face interview using a food frequency questionnaire. Setting Urban Shanghai, China. Participants 832 incident cases of endometrial cancer in women aged of 30 to 69 years diagnosed during 1997-2001 and identified from the Shanghai Cancer Registry; 846 control women frequency matched to cases on age and randomly selected from the Shanghai Residential Registry. Main outcome measures Odds ratios for risk of endometrial cancer in women with different intakes of soya foods. Results Regular consumption of soya foods, measured as amount of either soya protein or soya isoflavones, was inversely associated with the risk of endometrial cancer. Compared with women with the lowest quarter of intake, the adjusted odds ratio of endometrial cancer was reduced from 0.93 to 0.85 and 0.67 with increasing quarter of soya protein intake (P for trend 0.01). A similar inverse association was observed for soya isoflavones and soya fibre intake. The inverse association seemed to be more pronounced among women with high body mass index and waist:hip ratio. Conclusion Regular intake of soya foods is associated with a reduced risk of endometrial cancer. PMID:15136343

  1. MALDI-TOF MS analysis of labile Lolium perenne major allergens in mixes.

    Science.gov (United States)

    Irañeta, S G; Acosta, D M; Duran, R; Apicella, C; Orlando, U D; Seoane, M A; Alonso, A; Duschak, V G

    2008-08-01

    It is well known that allergen extracts used for specific therapy of allergic disorders are commonly stored as mixtures, causing an alteration of its stability. The aim of this report is to identify pollen allergens susceptible to degradation during storage of mixtures containing different sources of proteases in the absence of glycerol as a preserving agent. Mixes containing Lolium perenne (Lol p) pollen extract with either Aspergillus fumigatus or Periplaneta americana extracts were prepared and co-incubated for 90 days at 4 degrees C. Samples were taken off at fixed times and comparatively tested by in vitro and in vivo assays with atopic patients. Selected pollinic allergens were subjected to MALDI-TOF MS analysis. ELISA inhibition evidenced the loss of potency from ryegrass extract, and immunoblotting assays showed the degradation of specific pollinic allergens during storage of mixtures containing protease-rich sources. An in vivo intradermal skin assay confirmed the gradual loss of the biological activity of L. perenne pollen extract co-incubated with non-related protease-rich extracts in comparison with that of the control pollen extract. MALDI-TOF MS analysis allowed us to determine that Lol p 1 and Lol p 5 are susceptible to proteolysis whereas Lol p 4 was found to be resistant to degradation during storage. Lol p 1 and Lol p 5 degradation is responsible for the loss of the biological activity of L. perenne pollen extract when co-incubated with protease-rich fungal and cockroach extracts in the same vial for months in the absence of glycerol as a preserving agent. The integrity of these major allergens must be preserved to increase the vaccine stability and to assure efficacy when mixes are used for immunotherapy.

  2. Cross-species amplification of 105 Lolium perenne SSR loci in 23 species within the Poaceae

    DEFF Research Database (Denmark)

    Jensen, Louise Bach; Holm, Preben Bach; Lübberstedt, Thomas

    2007-01-01

    Amplification of 105 Lolium perenne SSR markers was studied in 23 grass species representing seven tribes from three subfamilies of Poaceae. Twelve of the SSR markers are published for the first time. Between 2% and 96% of the SSR markers could be amplified within a given species. A subset of eight...... SSR markers was evaluated for polymorphism across nine of the 23 grass species. Four to seven of the markers were polymorphic within each species, with an average detection of 2.4 alleles per species....

  3. Nitrogen application in amenity-types of Lolium perenne L. grown for seed production

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Boelt, Birte

    1998-01-01

    Three amenity-type perennial ryegrass (Lolium perenne) cultivars Elka, Taya and Pippin were undersown in 1991-93 in spring barley at Roskilde and Rnhave, Denmark, and given 0, 30 or 60 kg N ha-1 in autumn combined with 70, 100 or 130 kg N ha-1 in spring. Seed yield did not differ among cultiva......, and total seed yield was therefore not affected. The one exception was in 1991 when at one location dry matter production in autumn was low, and autumn N application resulted in increased N content, fertile tiller number and seed yield...

  4. Degradation assessment of natural weathering on low density polyethylene/thermoplastic soya spent powder blends

    Science.gov (United States)

    Nuradibah, M. A.; Sam, S. T.; Noriman, N. Z.; Ragunathan, S.; Ismail, H.

    2015-07-01

    Soya spent powder was blended with low density polyethylene (LDPE) ranging from 5-25 wt%. Glycerol was added to soya spent powder (SSP) for preparation of thermoplastic soya spent powder (TSSP). Then, the blends were exposed to natural weathering for 6 months. The susceptibility of the LDPE/soya spent powder blends based on its tensile, morphological properties and structural changes was measured every three months. The tensile strength of LDPE/TSSP blends after 6 months of weathering was the lowest compared to the other blends whereas LDPE/SSP blends after 6 months of weathering demonstrated the lowest elongation at break (Eb). Large pore can be seen on the surface of 25 wt% of LDPE/SSP blends.

  5. Effect of soya protein on blood pressure: a meta-analysis of randomised controlled trials.

    Science.gov (United States)

    Dong, Jia-Yi; Tong, Xing; Wu, Zhi-Wei; Xun, Peng-Cheng; He, Ka; Qin, Li-Qiang

    2011-08-01

    Observational studies have indicated that soya food consumption is inversely associated with blood pressure (BP). Evidence from randomised controlled trials (RCT) on the BP-lowering effects of soya protein intake is inconclusive. We aimed to evaluate the effectiveness of soya protein intake in lowering BP. The PubMed database was searched for published RCT in the English language through to April 2010, which compared a soya protein diet with a control diet. We conducted a random-effects meta-analysis to examine the effects of soya protein on BP. Subgroup and meta-regression analyses were performed to explore possible explanations for heterogeneity among trials. Meta-analyses of twenty-seven RCT showed a mean decrease of 2·21 mmHg (95 % CI - 4·10, - 0·33; P = 0·021) for systolic BP (SBP) and 1·44 mmHg (95 % CI - 2·56, - 0·31; P = 0·012) for diastolic BP (DBP), comparing the participants in the soya protein group with those in the control group. Soya protein consumption significantly reduced SBP and DBP in both hypertensive and normotensive subjects, and the reductions were markedly greater in hypertensive subjects. Significant and greater BP reductions were also observed in trials using carbohydrate, but not milk products, as the control diet. Meta-regression analyses further revealed a significantly inverse association between pre-treatment BP and the level of BP reductions. In conclusion, soya protein intake, compared with a control diet, significantly reduces both SBP and DBP, but the BP reductions are related to pre-treatment BP levels of subjects and the type of control diet used as comparison.

  6. Effect of γ-irradiation on soya bean proteins

    International Nuclear Information System (INIS)

    Byun, Myung-Woo; Kang, Il-Jun; Hayashi, Yukako; Matsumura, Yasuki; Mori, Tomohiko

    1993-01-01

    The properties of soya beans proteins following γ-irradiation (5-20 kGy) were investigated in connection with protein denaturation. Irradiation doses above 10 kGy caused a decrease in 7S and 11S components and an increase in 2S and 15S components (P < 0.05). However, subunit patterns determined by electrophoresis were not changed appreciably over the entire irradiation dose range. In the differential scanning calorimetry thermogram, the denaturation temperatures of 11S and 7S components were not affected by γ-irradiation, while increased irradiation dose caused a decrease in the enthalpy values of 11S and 7S components due to protein denaturation. Changes in the circular dichroism spectra and tryptophan fluorescence intensity (P < 0.05) were observed only at 20 kGy. (author)

  7. Soya Saponins Induce Enteritis in Atlantic Salmon (Salmo salar L.).

    Science.gov (United States)

    Krogdahl, Åshild; Gajardo, Karina; Kortner, Trond M; Penn, Michael; Gu, Min; Berge, Gerd Marit; Bakke, Anne Marie

    2015-04-22

    Soybean meal-induced enteritis (SBMIE) is a well-described condition in the distal intestine of salmonids, and saponins have been implicated as the causal agent. However, the question remains whether saponins alone cause SBMIE. Moreover, the dose-response relationship has not been described. In a 10 week feeding trial with Atlantic salmon, a highly purified (95%) soya saponin preparation was supplemented (0, 2, 4, 6, or 10 g/kg) to two basal diets, one containing fishmeal as the major protein source (FM) and the other 25% lupin meal (LP). Saponins caused dose-dependent increases in the severity of inflammation independent of the basal diet, with concomitant alterations in digestive functions and immunological marker expression. Thus, saponins induced inflammation whether the diet contained other legume components or not. However, responses were often the same or stronger in fish fed the corresponding saponin-supplemented LP diets despite lower saponin exposure, suggesting potentiation by other legume component(s).

  8. Fermented soya bean (tempe) extracts reduce adhesion of enterotoxigenic Escherichia coli to intestinal epithelial cells.

    Science.gov (United States)

    Roubos-van den Hil, P J; Nout, M J R; Beumer, R R; van der Meulen, J; Zwietering, M H

    2009-03-01

    This study aimed to investigate the effect of processed soya bean, during the successive stages of tempe fermentation and different fermentation times, on adhesion of enterotoxigenic Escherichia coli (ETEC) K88 to intestinal brush border cells as well as Caco-2 intestinal epithelial cells; and to clarify the mechanism of action. Tempe was prepared at controlled laboratory scale using Rhizopus microsporus var. microsporus as the inoculum. Extracts of raw, soaked and cooked soya beans reduced ETEC adhesion to brush border cells by 40%. Tempe extracts reduced adhesion by 80% or more. ETEC adhesion to Caco-2 cells reduced by 50% in the presence of tempe extracts. ETEC K88 bacteria were found to interact with soya bean extracts, and this may contribute to the observed decrease of ETEC adhesion to intestinal epithelial cells. Fermented soya beans (tempe) reduce the adhesion of ETEC to intestinal epithelial cells of pig and human origin. This reduced adhesion is caused by an interaction between ETEC K88 bacteria and soya bean compounds. The results strengthen previous observations on the anti-diarrhoeal effect of tempe. This effect indicates that soya-derived compounds may reduce adhesion of ETEC to intestinal cells in pigs as well as in humans and prevent against diarrhoeal diseases.

  9. Estimation of soya cultivation efficiency in conditions of Belarus lands polluted by radionuclides

    International Nuclear Information System (INIS)

    Gutseva, G.Z.

    2007-01-01

    Production of high-protein soya crop including lands polluted by radionuclides after the Chernobyl accident, causes to the necessity of carrying out research to study the radionuclide transfer into production of this crop. As a result of research the transfer factors of 137Cs and 90Sr from soil into seeds and green mass of various soya varieties have been determined to allow a prediction of radionuclide transfer into production. Limiting densities of radionuclide pollution for moderately improved sod - podzol sandy soils for production of the soya products corresponding of 137Cs and 90Sr content to the national permissible levels are established. Use of the crop in plant cultivation and cattle-breeding branch is accompanied by high energy - conserving effects. Soya products contains high quantity of total energy per kilogram of forage. The most highly energy-conserving forages are waste products of soya processing: an oil cake - up to 87,4 Mj and soil-seed meal up to 79,7 Mj. High profitability of this crop cultivation is provided by production for seeds. It is economically defensible a soya beans cultivation for processing and for fodder. For reception of seeds for food purposes there are restrictions on pollution of soil: 1125 kBk/square ? (30 Ci/square km) and 90Sr to 2,6 kBk/square ? (0,07 Ci/square km)

  10. Higher bioavailability of isoflavones after a single ingestion of a soya-based supplement than a soya-based food in young healthy males.

    Science.gov (United States)

    Vergne, Sébastien; Bennetau-Pelissero, Catherine; Lamothe, Valérie; Chantre, Philippe; Potier, Mylène; Asselineau, Julien; Perez, Paul; Durand, Marlène; Moore, Nicholas; Sauvant, Patrick

    2008-02-01

    Soya isoflavones, genistein and daidzein, are the focus of numerous studies investigating their potential effects on health and results remain controversial. Bioavailability is clearly a crucial factor influencing their bioefficacy and could explain these discrepancies. This study aimed at assessing: (1) the isoflavone content of sixty-nine European soya-derivative products sold on the French market; (2) the bioavailability of isoflavones comparing supplement with food. Twelve healthy volunteers were recruited in a randomized two-way crossover trial and received 35 mg isoflavones equivalent aglycone either through supplements or through cheese, both containing different patterns of isoflavone conjugates and different daidzein:genistein ratios. A specific ELISA method was used to assess the plasma and urinary concentrations of isoflavones and thus the pharmacokinetic parameters, which were then normalized to mg of each isoflavone ingested. Results showed that the normalized Cmax of daidzein (P = 0.002) and similarly the normalized AUC0 --> infinity and Cmax of genistein (P = 0.002) from soya-based capsules were higher than that from soya-based cheese. In conclusion, this work completes studies on isoflavone bioavailability and presents new data regarding isoflavone concentrations in soya-derivative products. Assuming that isoflavone conjugation patterns do not influence isoflavone bioavailability, this study shows that isoflavones contained in capsules are more bioavailable than those contained in soya-based cheese. Although the supplement is more bioavailable, the relative importance of this is difficult to interpret as there is little evidence that supplements are biologically active in human subjects to date and further studies will be necessary for this specific supplement to prove its efficacy.

  11. Effect of gamma irradiation on the protein, amino acids and carbohydrate contents of soya-gari diet

    International Nuclear Information System (INIS)

    Ogbadu, G.H.

    1979-01-01

    Soya-gari diet, prepared by enrichment of gari (Manihot esculanta Cranz) with soya flour, methionine, lysine and salt mixture was irradiated with doses of 62.5, 125, 250 and 500 Krad from a Co 60 γ irradiator. Gamma irradiation of the soya-gari diet with doses as high as 500 Krad had no significant effect on the amino acids, total proteins, soluble carbohydrates, hemicelluloses, cellulose and lignin contents. (author)

  12. EFECTO DEL PICADO SOBRE LAS CARACTERÍSTICAS NUTRICIONALES Y FERMENTATIVAS DE ENSILAJES DE PASTOS KIKUYO, RYEGRASS PERENNE Y ALPISTE FORRAJERO

    Directory of Open Access Journals (Sweden)

    Luis Villalobos

    2016-01-01

    Full Text Available Se evaluó el efecto del picado sobre las características nutricionales y fermentativas de ensilajes elaborados con 3 pastos (kikuyo, rye-grass perenne y alpiste forrajero. Se elaboraron 2 microsilos de cada pasto en bolsas de polieti-leno con capacidad para 1 kg, aplicando 2 trata-mientos (picado y sin picar; se adicionó a cada microsilo 3% de melaza y 0,1% de un inóculo artesanal (hecho de leche agria, suero de queso y melaza. La especie de pasto tuvo el mayor impacto sobre las características nutricionales y fermentativas de los microsilos, mientras que el picado no mostró tendencia clara sobre los parámetros evaluados. El contenido de PC (14,40 a 19,35%, FDN (46,25 a 63,16% y la DIVMS (46,15 a 65,55% en los ensilados es indicador de su potencial para llenar gran parte de los requerimientos nutricionales del ganado lechero. El pasto ryegrass ensilado alcanzó los niveles de pH (4,41 a 4,83 menores y el pasto kikuyo los valores menores de capacidad amor-tiguadora (79,12 a 81,17 mEq NaOH 100 g-1 MS y de nitrógeno amoniacal (1,03 a 1,33%. Los ensilajes elaborados con pastos pueden uti-lizarse como suplementos con valor nutricional superior a los henos utilizados en las raciones de ganado lechero en zonas altas.

  13. Aided Phytostabilization of Copper Contaminated Soils with L. Perenne and Mineral Sorbents as Soil Amendments

    Science.gov (United States)

    Radziemska, Maja

    2017-09-01

    The present study was designed to assess phytostabilization strategies for the treatment of soil co-contaminated by increasing levels of copper with the application mineral amendments (chalcedonite, zeolite, dolomite). From the results it will be possible to further elucidate the benefits or potential risks derived from the application of different types of mineral amendments in the remediation of a copper contaminated soil. A glasshouse pot experiment was designed to evaluate the potential use of different amendments as immobilizing agents in the aided phytostabilization of Cu-contaminated soil using ryegrass (Lolium perenne L.). The content of trace elements in plants and total in soil, were determined using the method of spectrophotometry. All of the investigated element contents in the tested parts of L. perenne were significantly different in the case of applying mineral amendments to the soil, as well as increasing concentrations of copper. The greatest average above-ground biomass was observed for soil amended with chalcedonite. In this experiment, all analyzed metals accumulated predominantly in the roots of the tested plant. In general, applying mineral amendments to soil contributed to decreased levels of copper concentrations.

  14. [Effects of loess soil stabilization on Lolium perenne L. growth and root activity].

    Science.gov (United States)

    Liu, Yue-mei; Zhang, Xing-chang; Wang, Dan-dan

    2011-10-01

    Taking the loess soils with bulk density 1.2 g cm(-3), 1.3 g cm(-3), and 1.4 g cm(-3) from Ansai, Shaanxi Province as test objects, a pot experiment was conducted to study the effects of different amendment amount of soil stabilizer (EN-1 stabilizer) on the growth and root activity of ryegrass (Lolium perenne L.). Within the range of the bulk densities, the leaf chlorophyll content, root activity, root/shoot ratio, root biomass, and plant biomass of L. perenne all decreased with increasing soil bulk density, and were higher under the amendment of EN-1 stabilizer, as compared with the control. With increasing amendment amount of EN-1 stabilizer, the leaf chlorophyll content, root activity, root/shoot ratio, root biomass, and plant biomass had a trend of increased first and decreased then. Soil bulk density and stabilizer amendment amount had significant interactive effect on the root biomass and plant biomass. Overall, the values of the test indices were the highest under 1.3 g cm(-3) soil bulk density and 0.15% EN-1 stabilizer amendment amount.

  15. Metabolite profiling during cold acclimation of Lolium perenne genotypes distinct in the level of frost tolerance.

    Science.gov (United States)

    Bocian, Aleksandra; Zwierzykowski, Zbigniew; Rapacz, Marcin; Koczyk, Grzegorz; Ciesiołka, Danuta; Kosmala, Arkadiusz

    2015-11-01

    Abiotic stresses, including low temperature, can significantly reduce plant yielding. The knowledge on the molecular basis of stress tolerance could help to improve its level in species of relatively high importance to agriculture. Unfortunately, the complex research performed so far mainly on model species and also, to some extent, on cereals does not fully cover the demands of other agricultural plants of temperate climate, including forage grasses. Two Lolium perenne (perennial ryegrass) genotypes with contrasting levels of frost tolerance, the high frost tolerant (HFT) and the low frost tolerant (LFT) genotypes, were selected for comparative metabolomic research. The work focused on the analysis of leaf metabolite accumulation before and after seven separate time points of cold acclimation. Gas chromatography-mass spectrometry (GC/MS) was used to identify amino acids (alanine, proline, glycine, glutamic and aspartic acid, serine, lysine and asparagine), carbohydrates (fructose, glucose, sucrose, raffinose and trehalose) and their derivatives (mannitol, sorbitol and inositol) accumulated in leaves in low temperature. The observed differences in the level of frost tolerance between the analysed genotypes could be partially due to the time point of cold acclimation at which the accumulation level of crucial metabolite started to increase. In the HFT genotype, earlier accumulation was observed for proline and asparagine. The increased amounts of alanine, glutamic and aspartic acids, and asparagine during cold acclimation could be involved in the regulation of photosynthesis intensity in L. perenne. Among the analysed carbohydrates, only raffinose revealed a significant association with the acclimation process in this species.

  16. Inability to detect significant absorption of immunoreactive soya protein in healthy adults may be relevant to its weak allergenicity

    DEFF Research Database (Denmark)

    Lund, Cecilia M; Dirks, Christina G; Pedersen, Mona H

    2013-01-01

    ABSTRACT: Soya and peanut are botanically closely related and share cross-reacting antigens, but compared to soya, peanut allergy has a higher prevalence with more severe allergic reactions. Furthermore, the threshold dose for eliciting reactions is higher for soya. A difference in undigested...... of soya protein. While we cannot totally exlude technical reasons, it may also reflect a true poor absorption in healthy adult volunteers. This could, in turn, be relevant to the apparently weak allergenicity of soy protein by comparison with peanut protein in allergic subjects....

  17. Elevated atmospheric carbon dioxide concentration: effects of increased carbon input in a Lolium perenne soil on microorganisms and decomposition

    NARCIS (Netherlands)

    Ginkel, van J.H.; Gorissen, A.; Polci, D.

    2000-01-01

    Effects of ambient and elevated atmospheric CO2 concentrations (350 and 700 μl l-1) on net carbon input into soil, the production of root-derived material and the subsequent microbial transformation were investigated. Perennial ryegrass plants (L. perenne L.) were labelled in a continuously labelled

  18. Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

    Czech Academy of Sciences Publication Activity Database

    Begheyn, R. F.; Roulund, N.; Vangsgaard, K.; Kopecký, David; Studer, B.

    2017-01-01

    Roč. 130, č. 3 (2017), s. 667-679 ISSN 0167-6857 Institutional support: RVO:61389030 Keywords : Androgenesis * Androgenic capacity * Anther culture * Doubled haploid (DH) * Perennial ryegrass (Lolium perenne L.) Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Plant sciences, botany Impact factor: 2.002, year: 2016

  19. Strategic timing of nitrogen fertilization to increase root biomass and nitrogen-use efficiency of Lolium perenne L.

    NARCIS (Netherlands)

    Boer, de H.C.; Deru, J.G.C.; Hoekstra, N.J.; Eekeren, van N.

    2016-01-01

    Aims: During the first days after harvest of Lolium perenne L., N remobilized from roots and stubble forms the main N source for regrowth. Low N uptake from the soil during this period may lead to N loss if N fertilizer is applied too soon. Furthermore, temporary N deprivation has been found to

  20. The annual variation in stomatal ammonia compensation point of rye grass (Lolium perenne L.) leaves in an intensively managed grassland

    NARCIS (Netherlands)

    Hove, van L.W.A.; Heeres, P.; Bossen, M.E.

    2002-01-01

    The stomatal ammonia compensation point for ammonia (NH3) of an intensively managed pasture of rye grass (Lolium perenne L.) was followed from mid January till November 2000. Leaf samples were taken every week. Simultaneously, the ambient NH3 concentration was measured. Meteorological data

  1. Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Studer, Bruno; Asp, Torben; Frei, Ursula

    2008-01-01

    An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was te...

  2. Carbon balance and water use efficiency of frequently cut Lolium perenne L. swards at elevated carbon dioxide

    NARCIS (Netherlands)

    Schapendonk, A.H.C.M.; Dijkstra, P.; Groenwold, J.; Pot, C.S.; Geijn, van de S.C.

    1997-01-01

    The impact of doubled atmospheric [CO2] on the carbon balance of regularly cut Lolium perenne L. swards was studied for two years under semi-field conditions in the Wageningen Rhizolab. CO2 and H2O vapour exchange rates of the swards were measured continuously for two years in transparent

  3. Development of soya milk extender for semen cryopreservation of Karan Fries (crossbreed cattle).

    Science.gov (United States)

    Singh, V K; Singh, A K; Kumar, R; Atreja, S K

    2013-01-01

    Egg yolk based semen extenders are used widely, with the potential risk of xenobiotic contamination. This study was designed to develop a soya milk based extender to substitute egg yolk based extender for bovine semen cryopreservation. In the first experiment soya milk was prepared from fresh soya bean (Glycine max). Concentration of soya milk in tris based extender was standardized based on quality parameters of spermatozoa during liquid preservation at 5°C up to 72 h and compared with egg yolk tris (EYT) extender. Sperm in soya milk tris (SMT) extender with 25 percent soya milk showed no significant (P > 0.05) differences in all the quality parameters like motility, viability, membrane integrity and acrosome integrity, as compared to sperm in EYT extender up to 72h in liquid dilution. In the second experiment the Karan Fries semen was cryopreserved in SMT extender with 25 percent soya milk (selected from the first experiment) using different concentration of glycerol, as cryoprotectant, ranging from 6-7 percent with a difference of 0.2 percent to standardize optimum concentration based on post thaw motility of spermatozoa. Glycerol at a final concentration of 6.4 percent was found to be the best among all. Further, semen samples were split and cryopreserved in newly developed SMT extender containing 6.4 percent glycerol and compared with conventional EYT extender for post thaw sperm quality parameters and degree of cryocapacitation. There were no significant (P > 0.05) differences between sperm in EYT extender and SMT extender for post thaw motility, viability, membrane integrity, acrosome integrity and cryocapacitation. In conclusion, the newly developed SMT extender maintained comparable semen quality as compared to EYT extender hence it can.

  4. Factores que influyen en el contenido de escualeno, fitoesteroles totales y esterificados en el subproducto ácidos grasos destilados de soya para su potencial aprovechamiento

    Directory of Open Access Journals (Sweden)

    José Rodríguez Rodríguez

    2015-01-01

    Full Text Available Una necesidad actual de la industria de extracción y refinación de aceite vegetal es la reducción y/o explotación de sus subproductos. Uno de estos, conocido como Ácidos Grasos Destilados de Aceite de Soya (AGDAS, resulta potencialmente valioso para las industrias alimentaria, farmacéutica y cosmética por la presencia de compuestos con actividad biológica favorable, como los fitoesteroles y el escualeno, los primeros como agentes hipocolesterolemiantes y el escualeno como emoliente y humectante. En este trabajo, mediante cromatografía de gases se determinó el contenido de escualeno y fitoesteroles libres y esterificados para evaluar la viabilidad de aprovechamiento de AGDAS como fuente de dichos compuestos y establecer si la procedencia del grano de soya (americano o nacional, la condición de cultivo (Sequía o Normal, años 2011 y 2012 respectivamente y el grado de calidad del aceite (Genérico o Premium son factores determinantes en el contenido de dichos compuestos en el subproducto. En conclusión AGDAS representa una fuente viable de fitoesteroles totales con 14.92% p/p, no así de escualeno con 1.75% p/p. La concentración de escualeno está influenciada por la procedencia del grano y la condición de cultivo, mientras que la de fitoesteroles esterificados por el grado de calidad y condición de cultivo. Ninguno de los factores influyó en el contenido de fitoesteroles totales. Esto permite establecer que la composición del subproducto AGDAS no es constante y se justifican protocolos como el de este estudio para su caracterización con fines de aprovechamiento.

  5. Comparison of human IgE-binding soya bean allergenic protein Gly m I with the antigenicity profiles of calf anti-soya protein sera

    NARCIS (Netherlands)

    Hessing, M.; Bleeker, H.; Tsuji, H.; Ogawa, T.; Vlooswijk, R.A.A.

    1996-01-01

    In the food, particularly the feed, industry, large quantities of soya bean protein products are used for the formulation of end-products destined for human or animal consumption. These basic ingredients and the final end products often have to fulfill specific requirements regarding the presence of

  6. Soya bean expansion in Mozambique : Exploring the inclusiveness and viability of soya business models as an alternative to the land grab

    NARCIS (Netherlands)

    di Matteo, F.|info:eu-repo/dai/nl/371570204; Otsuki, K.|info:eu-repo/dai/nl/306279258; Schoneveld, G.

    2016-01-01

    Throughout the world, soya beans and seeds are typically cultivated in large plantations. The way plantations have been established are often discussed as what represent the ‘global land grab’. Little has been discussed, however, about how large plantations evolve as they interact with various

  7. Sticking power from soya beans : higher fossil fuel prices and concerns over formaldehyde in existing glue formulations have led to a resurgence in interest in soya-based adhesives, report

    Science.gov (United States)

    James Wescott; Charles Frihart

    2011-01-01

    Soya beans have long been considered a miracle crop for their ease of growing; high concentration of vegetable oil and high protein content; and their ability to reintroduce nitrogen back into the soil. The origin of soya beans dates back to the early 11th century from the eastern half of China. They were introduced to Europe in 1712 and first...

  8. Identity recognition in response to different levels of genetic relatedness in commercial soya bean

    Science.gov (United States)

    Van Acker, Rene; Rajcan, Istvan; Swanton, Clarence J.

    2017-01-01

    Identity recognition systems allow plants to tailor competitive phenotypes in response to the genetic relatedness of neighbours. There is limited evidence for the existence of recognition systems in crop species and whether they operate at a level that would allow for identification of different degrees of relatedness. Here, we test the responses of commercial soya bean cultivars to neighbours of varying genetic relatedness consisting of other commercial cultivars (intraspecific), its wild progenitor Glycine soja, and another leguminous species Phaseolus vulgaris (interspecific). We found, for the first time to our knowledge, that a commercial soya bean cultivar, OAC Wallace, showed identity recognition responses to neighbours at different levels of genetic relatedness. OAC Wallace showed no response when grown with other commercial soya bean cultivars (intra-specific neighbours), showed increased allocation to leaves compared with stems with wild soya beans (highly related wild progenitor species), and increased allocation to leaves compared with stems and roots with white beans (interspecific neighbours). Wild soya bean also responded to identity recognition but these responses involved changes in biomass allocation towards stems instead of leaves suggesting that identity recognition responses are species-specific and consistent with the ecology of the species. In conclusion, elucidating identity recognition in crops may provide further knowledge into mechanisms of crop competition and the relationship between crop density and yield. PMID:28280587

  9. Seasonal Variation of Provitamin D2 and Vitamin D2 in Perennial Ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Jäpelt, Rie Bak; Didion, Thomas; Smedsgaard, Jørn

    2011-01-01

    Ergosterol (provitamin D(2)) is converted to vitamin D(2) in grass by exposure to UV light. Six varieties of perennial ryegrass (Lolium perenne L.) were harvested four times during the season, and the contents of vitamin D(2) and ergosterol were analyzed by a sensitive and selective liquid...... chromatography tandem mass spectrometry method. Weather factors were recorded, and a principal component analysis was performed to study which factors were important for the formation of vitamin D(2). The results suggest that a combination of weather factors is involved and that the contents of ergosterol...... and vitamin D(2) change more than a factor of 10 during the season. These results demonstrate that grass potentially can be a significant source of vitamin D for grazing animals and animals fed on silage and hay....

  10. Genetic characterisation of seed yield and fertility traits in perennial ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Studer, Bruno; Jensen, Louise Bach; Hentrup, Stephan

    2008-01-01

    A population. Path analysis partitioned the direct and indirect effects of seed yield components on seed yield per plant. Seed yield per panicle showed the highest effect on total seed yield. The adjusted mean values of each trait and a genetic linkage map consisting of 97 anonymous and 85 gene associated DNA......Seed yield is a trait of major interest for the key grassland species Lolium perenne L. An F2 mapping population of perennial ryegrass (VrnA), recently characterised for vernalisation response, was assessed in a glasshouse for traits related to seed yield based on a lattice design with four...... replications over 2 years. The traits heading date, plant height, length of panicles, number of panicles per plant, seed yield per panicle, flag leaf length, flag leaf width and seed yield per plant revealed repeatabilities ranging from 41 to 76% and a considerable amount of genetic variation in the Vrn...

  11. Gamma-ray sterilization of peat carriers for the production of legume seed inoculants (better production of soya bean)

    International Nuclear Information System (INIS)

    Vojinovic, Z.; Milicic, B.; Radak, B.

    1983-01-01

    In the present work the first Yugoslav experiments on the application of gamma radiation for the sterilization of peat in the production of inoculants for soya bean production are summarised and briefly discussed. A radiation dose of 50 kGy was used. The growth and survival of the soya bean nodule bacteria, Rhizobium japonicum, in the sterilized peat is shown. (U.K.)

  12. Granulomatous enteritis in rainbow trout (Oncorhynchus mykiss) associated with soya bean meal regardless of water dissolved oxygen level

    NARCIS (Netherlands)

    Mosberian-Tanha, P.; Landsverk, T.; Press, C.M.; Mydland, L.T.; Schrama, J.W.; Øverland, M.

    2018-01-01

    This study investigated morphological changes associated with soya bean meal-induced enteritis (SBMIE) in distal intestine (DI) of rainbow trout (Oncorhynchus mykiss) fed a soya bean meal (SBM)-based diet and exposed to normoxia or hypoxia created by optimal and low water flow rates, respectively. A

  13. 40 CFR 721.7270 - 1-propanaminium, 3-amino-, N,N,N-trimethyl-N-soya acyl derivs., chloride.

    Science.gov (United States)

    2010-07-01

    ...-trimethyl-N-soya acyl derivs., chloride. 721.7270 Section 721.7270 Protection of Environment ENVIRONMENTAL...-soya acyl derivs., chloride. (a) Chemical substance and significant new uses subject to reporting. (1...., chloride (PMN P-01-646; CAS No. 391232-99-8) is subject to reporting under this section for the significant...

  14. Cloning, expression, and immunological characterization of recombinant Lolium perenne allergen Lol p II.

    Science.gov (United States)

    Sidoli, A; Tamborini, E; Giuntini, I; Levi, S; Volonté, G; Paini, C; De Lalla, C; Siccardi, A G; Baralle, F E; Galliani, S

    1993-10-15

    The molecular cloning of the cDNA encoding for an isoallergenic form of Lol p II, a major rye grass (Lolium perenne) pollen allergen, was performed by polymerase chain reaction amplification on mRNA extracted from pollen. The amino acid sequence derived from the cDNA was truncated by 4 and 5 residues at the NH2- and COOH-terminal ends, respectively, and differed only in one position from that previously reported. This cDNA was expressed in Escherichia coli by fusion to the carboxyl terminus of the human ferritin H-chain. The molecule was produced in high yields as a soluble protein and was easily purified. The protein retains the multimeric quaternary structure of ferritin, and it exposes on the surface the allergenic moiety, which can be recognized in Western blotting and in enzyme-linked immunosorbent assay experiments by specific IgE from allergic patients. The recombinant allergen was used to analyze the sera of 26 patients allergic to L. perenne compared with control sera. The results were in good agreement with the values obtained with the radioallergosorbent test assay. In addition, histamine release experiments in whole blood from an allergic patient and skin prick tests showed that the recombinant allergen retains some of the biological properties of the natural compound. These findings indicate that the availability of homogeneous recombinant allergens may be useful for the development of more specific diagnostic and therapeutic procedures. Moreover, this expression system may be of more general interest for producing large amounts of soluble protein domains in E. coli.

  15. Distribution of soya-saponin in brain and peripheral tissue after peritoneal injection

    International Nuclear Information System (INIS)

    Zhu Shigong; Wang Jianchun; Zhang Peiyin

    1997-01-01

    125 I-soya-saponin was prepared to study the distribution of soya-saponin in body of rat, as well as in different areas of brain when peritoneal injection. The results showed that the peak value of radioactive soya-saponin in all tissue appeared at 30 min after peritoneal injection. There were higher radioactivities in brain and suprarene comparing with other organs. The highest radioactivity was seen in hypothalamus among the every brain areas. It is a first report that soyasaponin can pass through the blood brain barrier when peripheral injection. The result also supported the opinion that soyasaponin might act on the hypothalamus and central regulation of cardiovascular system. Another finding was that soyasaponin also showed a higher affinity with adrenal gland, which indicated that the soyasaponin might possess of peripheral effect for regulation of cardiovascular system as well

  16. Grain product of 34 soya mutant lines;Rendimiento de grano de 34 lineas mutantes de soya

    Energy Technology Data Exchange (ETDEWEB)

    Salmeron E, J.; Mastache L, A. A.; Valencia E, F.; Diaz V, G. E. [Colegio Superior Agropecuario del Estado de Guerrero, Vicente Guerrero No. 81, Col. Centro, 40000 Iguala, Guerrero (Mexico); Cervantes S, T. [Instituto de Recursos Geneticos y Productividad, Colegio de Posgraduados, Carretera Mexico-Texcoco Km. 36.5, Montecillo, 56230 Texcoco, Estado de Mexico (Mexico); De la Cruz T, E.; Garcia A, J. M.; Falcon B, T.; Gatica T, M. A. [ININ, Departamento de Biologia, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico)

    2009-07-01

    This work was development with the objective of obtaining information of the agronomic behavior of 34 soya mutant lines (R{sub 4}M{sub 18}) for human consumption and this way to select the 2 better lines. The genetic materials were obtained starting from the variety ISAAEG-B M2 by means of the application of recurrent radiation with Co{sup 60} gammas, to a dose of 350 Gray for the first two generations and both later to 200 Gray and selection during 17 cycles, being obtained the 34 better lines mutants with agronomic characteristic wanted and good flavor. The obtained results were that the mutant lines L{sub 25} and L{sub 32} produced the major quantity in branches/plant number with 7.5 and 7.25, pods/plant number with 171.25 and 167, grains/plant number with 350.89 and 333.07 and grain product (ton/ha) to 15% of humidity 5.15 and 4.68 ton/ha, respectively. (Author)

  17. The association between soya consumption and serum thyroid-stimulating hormone concentrations in the Adventist Health Study-2.

    Science.gov (United States)

    Tonstad, Serena; Jaceldo-Siegl, Karen; Messina, Mark; Haddad, Ella; Fraser, Gary E

    2016-06-01

    Consumers may choose soya foods as healthful alternatives to animal products, but concern has arisen that eating large amounts of soya may adversely affect thyroid function. The present study aimed to examine the association between soya food consumption and serum thyroid-stimulating hormone (TSH) concentrations in North American churchgoers belonging to the Seventh-day Adventist denomination that encourages vegetarianism. Participants completed six repeated 24 h dietary recalls within a 6-month period. Soya protein and soya isoflavone intakes were estimated, and their relationships to TSH concentrations measured at the end of 6 months were calculated using logistic regression analyses. Calibration sub-study of the Adventist Health Study-2. Women (n 548) and men (n 295) who were not taking thyroid medications. In men, age and urinary iodine concentrations were associated with high serum TSH concentrations (>5 mIU/l), while among women White ethnicity was associated with high TSH. In multivariate models adjusted for age, ethnicity and urinary iodine, soya isoflavone and protein intakes were not associated with high TSH in men. In women higher soya isoflavone consumption was associated with higher TSH, with an adjusted odds ratio (highest v. lowest quintile) of 4·17 (95 % CI 1·73, 10·06). Likewise, women with high consumption of soya protein (midpoint of highest quintile, 11 g/d) v. low consumption (midpoint of lowest quintile, 0 g/d) carried increased odds of high TSH (OR=2·69; 95 % CI 1·34, 5·30). In women high consumption of soya was associated with elevated TSH concentrations. No associations between soya intake and TSH were found in men.

  18. Effect of soya lecithin on the enzymatic system of the white-rot fungi Anthracophyllum discolor.

    Science.gov (United States)

    Bustamante, M; González, M E; Cartes, A; Diez, M C

    2011-01-01

    The present work optimized the initial pH of the medium and the incubation temperature for ligninolytic enzymes produced by the white-rot fungus Anthracophyllum discolor. Additionally, the effect of soya lecithin on mycelial growth and the production of ligninolytic enzymes in static batch cultures were evaluated. The critical micelle concentration of soya lecithin was also studied by conductivity. The effects of the initial pH (3, 4, and 5) and incubation temperature (20, 25, and 30°C) on different enzymatic activities revealed that the optimum conditions to maximize ligninolytic activity were 26°C and pH 5.5 for laccase and manganese peroxidase (MnP) and 30°C and pH 5.5 for manganese-independent peroxidase (MiP). Under these culture conditions, the maximum enzyme production was 10.16, 484.46, and 112.50 U L(-1) for laccase, MnP, and manganese-independent peroxidase MiP, respectively. During the study of the effect of soya lecithin on A. discolor, we found that the increase in soya lecithin concentration from 0 to 10 g L(-1) caused an increase in mycelial growth. On the other hand, in the presence of soya lecithin, A. discolor produced mainly MnP, which reached a maximum concentration of 30.64 ± 4.61 U L(-1) after 25 days of incubation with 1 g L(-1) of the surfactant. The other enzymes were produced but to a lesser extent. The enzymatic activity of A. discolor was decreased when Tween 80 was used as a surfactant. The critical micelle concentration of soya lecithin calculated in our study was 0.61 g L(-1).

  19. Efecto del nivel de inclusión de soya en la digestibilidad in vitro de la harina de piscidium de Moringa oleifera Effect of the soybean inclusion rate on the in vitro digestibility of the meal from Moringa oleifera pods

    Directory of Open Access Journals (Sweden)

    I. L Montejo

    2012-06-01

    Full Text Available El trabajo tuvo como objetivo evaluar el efecto de la inclusión de harina de soya, en forma de reactivo para simular el efecto de un concentrado proteínico, en la digestibilidad in vitro de la harina de piscidium (vainas de moringa. Se evaluaron nueve tratamientos de piscidium de moringa:soya (100:0; 90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 20:80 y 0:100, con un diseño completamente aleatorizado y tres réplicas por cada tratamiento. Se observó un incremento en la producción de gas con el aumento del porcentaje de inclusión de soya; la digestibilidad verdadera tuvo un comportamiento similar. En cambio, la producción de biomasa microbiana alcanzó su mayor valor con la inclusión de la soya al 10%. Se concluye que la adición de la soya mejora la digestibilidad de las vainas de moringa, y que la eficiencia óptima de aprovechamiento de esta dieta se alcanza con la inclusión del 10% de soya.The objective of this work was to evaluate the effect of the inclusion of soybean meal, in this case in a way of a lab reactive, simulating a conventional protein concentrate, on the in vitro digestibility of the meal from Moringa oleifera pods. Nine treatments of M. oleifera pods:soybean were evaluated (100:0, 90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 20:80 and 0:100, with a completely randomized design and three replications per treatment. An increase was observed in gas production with the increase of the soybean inclusion percentage; the true digestibility had a similar performance. On the contrary, the production of microbial biomass reached its higher value with the inclusion of 10% soybean. The addition of soybean is concluded to improve the digestibility of the M. oleifera pods, and the optimum utilization efficiency of this diet is reached with the inclusion of 10% soybean.

  20. Evaluación agronómica y nutricional del pasto Ryegrass perenne tetraploide (Lolium perenne producido en lecherías de las zonas altas de Costa Rica. II. Valor nutricional

    Directory of Open Access Journals (Sweden)

    Luis Villalobos

    2010-01-01

    Full Text Available Se determinó el valor nutricional del pasto ryegrass perenne tetraploide (Lolium perenne en 4 fincas comerciales de ganado lechero ubicadas en Chicuá de Oreamuno (latitud 09°59', longitud 83°52', altitud 3090 msnm, provincia de Cartago. Las muestras se tomaron cada 2 meses a una altura de cosecha de 10 cm sobre el suelo, simulando el pastoreo que realizan los animales durante un período de un año. La composición nutricional promedio anual fue de 25,21% PC, 46,26% FDN, 25,57% FDA, 3,29% lignina, 15,40% CNF y 77,95% DIVMS y su contenido energético, expresado como TND, ED, EM, ENL (3X y ENG, fue 61,95%, 2,92, 2,45, 1,53 y 0,92 Mcal.kg-1 de MS, respectivamente. El valor nutricional del pasto ryegrass perenne varió (p¿0,05 según la época del año y el manejo en finca, especialmente en lo referente al período de recuperación de las pasturas. Este pasto tiene un contenido alto de PC y CNF, que permite a nivel ruminal la producción de proteína microbial, la cual es la mejor proteína que puede consumir un rumiante. En general, el valor nutricional del pasto Ryegrass perenne producido a altitudes superiores a los 2500 msnm en Costa Rica es similar, al producido en zonas de clima templado de donde es originario.

  1. Soya protein antibodies in man: their occurrence and possible relevance in coeliac disease.

    Science.gov (United States)

    Haeney, M R; Goodwin, B J; Barratt, M E; Mike, N; Asquith, P

    1982-01-01

    Circulating antibodies to soya-derived protein antigens have been measured in patients with duodenitis, Crohn's disease, ulcerative colitis and coeliac disease. Significantly raised antibody titres were found frequently in the coeliac group, particularly those patients showing a suboptimal response to a gluten-free diet, but rarely in subjects with other gastrointestinal diseases. Antisoya activity was not necessarily accompanied by antibodies to other common dietary antigens. We suggest that some coeliacs may have an associated dietary soya sensitivity which could adversely influence their response to gluten withdrawal. PMID:7040491

  2. Soya-saponins induce intestinal inflammation and barrier dysfunction in juvenile turbot (Scophthalmus maximus).

    Science.gov (United States)

    Gu, Min; Jia, Qian; Zhang, Zhiyu; Bai, Nan; Xu, Xiaojie; Xu, Bingying

    2018-06-01

    Soybean meal-induced enteritis (SBMIE) is a well-described condition in the distal intestine (DI) of several cultured fish species, but the exact cause is still unclear. The work on Atlantic salmon and zebrafish suggested soya-saponins, as heat-stable anti-nutritional factors in soybean meal, are the major causal agents. However, this conclusion was not supported by the research on some other fish, such as gilthead sea bream and European sea bass. Our previous work proved that soybean could induce SBMIE on turbot and the present work aimed to investigate whether soya-saponins alone could cause SBMIE and the effects of soya-saponins on the intestinal barrier function in juvenile turbot. Turbots with initial weight 11.4 ± 0.02 g were fed one of four fishmeal-based diets containing graded levels of soya-saponins (0, 2.5, 7.5, 15 g kg -1 ) for 8 weeks. At the end of the trial, all fish were weighed and plasma was obtained for diamine oxidase (DAO) activity and d-lactate level analysis and DI was sampled for histological evaluation and quantification of antioxidant parameters and inflammatory marker genes. The activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and intestinal glutathione level were selected to evaluated intestinal antioxidant system. The distal intestinal epithelial cell (IEC) proliferation and apoptosis were investigated by proliferating cell nuclear antigen (PCNA) labelling and TdT-mediated dUTP nick end labeling (TUNEL), respectively. The results showed that soya-saponins caused significantly dose-dependent decrease in the growth performance and nutrient utilization (p soya-saponins. Significantly dose-dependent increases in severity of the inflammation concomitant with up-regulated expression of il-1β, il-8, and tnf-α, increased IEC proliferation and apoptosis, and decreases in selected antioxidant parameters were detected (p soya-saponins (p soya-saponins induced enteritis and compromised

  3. Effects of inclusion level on nutrient digestibility and energy content of wheat middlings and soya bean meal for growing pigs.

    Science.gov (United States)

    Huang, Qiang; Piao, Xiangshu; Liu, Ling; Li, Defa

    2013-01-01

    Two experiments were conducted to determine the effects of inclusion level of wheat middlings and soya bean meal on apparent total tract digestibility (ATTD) of energy and chemical components of these ingredients in growing pigs. Furthermore, the effects of the inclusion level on their contents of digestible energy (DE) and metabolisable energy (ME) were also determined by the difference method. In Experiment 1, six diets were fed to 36 growing pigs according to a completely randomised design. The basal diet was a corn-soya bean meal diet while the other five diets contained 9.6%, 19.2%, 28.8%, 38.4% or 48.0% of wheat middlings added at the expense of corn and soya bean meal. The measured digestibility of energy and most nutrients of wheat middlings increased (p soya bean meal (22.2% and 33.6%). The content of DE in soya bean meal did not differ at 22.2% and 33.6% inclusion levels (16.2 and 16.3 MJ/kg DM, respectively), but the digestibility of dry matter (DM), organic matter and carbohydrates was increased at a higher inclusion level (p soya bean meal and wheat middlings was affected by their dietary inclusion levels. For soya bean meal, the estimated energy contents was independent of its inclusion level, but not for wheat middlings. Therefore, the inclusion level of wheat middlings has to be considered for estimating their energy value.

  4. Relative efficacy of casein or soya protein combined with palm or safflower-seed oil on hyperuricaemia in rats.

    Science.gov (United States)

    Lo, Hui-Chen; Wang, Yao-Horng; Chiou, Hue-Ying; Lai, Shan-Hu; Yang, Yu

    2010-07-01

    Diets that ameliorate the adverse effects of uric acid (UA) on renal damage deserve attention. The effects of casein or soya protein combined with palm or safflower-seed oil on various serum parameters and renal histology were investigated on hyperuricaemic rats. Male Wistar rats administered with oxonic acid and UA to induce hyperuricaemia were fed with casein or soya protein plus palm- or safflower-seed oil-supplemented diets. Normal rats and hyperuricaemic rats with or without allopurinol treatment (150 mg/l in drinking water) were fed with casein plus maize oil-supplemented diets. After 8 weeks, allopurinol treatment and soya protein plus safflower-seed oil-supplemented diet significantly decreased serum UA in hyperuricaemic rats (one-way ANOVA; P soya protein and casein attenuated hyperuricaemia-induced decreases in serum albumin and insulin, respectively (two-way ANOVA; P soya protein significantly decreased renal NO and nitrotyrosine and palm oil significantly decreased renal nitrotyrosine, TNF-alpha and interferon-gamma and increased renal transforming growth factor-beta. Casein with safflower-seed oil significantly attenuated renal tubulointerstitial nephritis, crystals and fibrosis. Comparing casein v. soya protein combined with palm or safflower-seed oil, the results support that casein with safflower-seed oil may be effective in attenuating hyperuricaemia-associated renal damage, while soya protein with safflower-seed oil may be beneficial in lowering serum UA and TAG.

  5. Evaluation of Perennial Forage Legumes and Herbs in Six Mediterranean Environments Evaluación de Leguminosas y Hierbas Forrajeras Perennes en Seis Medioambientes Mediterráneos

    Directory of Open Access Journals (Sweden)

    Daniel Real

    2011-09-01

    Full Text Available There is an absence of drought tolerant herbaceous perennial forage legume and herb options other than lucerne (Medicago sativa L. for environments with Mediterranean-like climates common in extensive areas of Southern Australia, the Mediterranean basin, and Chile. Therefore, a collection of 174 forage perennial legume and herb entries from 103 species and 32 genera was evaluated for adaptation in a diverse range of Mediterranean climatic environments in Southern Australia. The seasonal rainfall distribution varied from moderately to highly winter dominant with long term average annual rainfall ranging from 318 to 655 mm. The entries were rated for productivity and persistence over 3 yr. The 12 entries identified as the most promising for winter, summer, or all-year round production included Bituminaria bituminosa (L. C.H. Stirt. var. albomarginata; Cichorium intybus L.; Cullen australasicum (Schltdl. J.W. Grimes; Dorycnium hirsutum (L. Ser.; Kennedia prostrata R. Br.; Lotononis bainesii Baker, Lotus pedunculatus Cav.; L. corniculatus L.; L. cytisoides L.; Medicago sativa subsp. sativa L.; Medicago sativa subsp. caerulea (Less. ex Ledeb. Schmalh., and M. sativa subsp. falcata (L. Arcang. These entries maintained production and persisted for the period of the evaluation, with the exception of C. intybus and L. corniculatus that declined in persistence over time. The potential role of these species in extensive grazing systems in Mediterranean climatic zones, their attributes and limitations, and current progress in developing them as useful forage plants was discussed.Existe una escasez de leguminosas y hierbas perennes herbáceas además de alfalfa (Medicago sativa L. tolerantes a sequía para ambientes con clima mediterráneo como los que se encuentran en el Sur de Australia, el Mediterráneo y Chile. Por lo tanto, una colección de 174 leguminosas perennes y hierbas correspondientes a 103 especies y 32 géneros fue evaluada por su adaptaci

  6. Influencia del tipo e intensidad de luz en la formación y multiplicación de embriones somáticos de soya

    Directory of Open Access Journals (Sweden)

    Jorge Liusvert Pérez Pérez

    2012-07-01

    Full Text Available Título en ingles: Influence of light type and intensity on induction and proliferation of somatic embryos in soybean Resumen: La embriogénesis somática representa una alternativa para la regeneración de plantas, sobre la cual influyen diversos factores durante el cultivo in vitro. Entre estos, las condiciones de iluminación durante la formación de embriones somáticos es determinante. Sin embargo, no existen trabajos que refieran el efecto de la luz solar en la formación de embriones somáticos de soya. Este trabajo tuvo como objetivo evaluar el efecto del tipo e intensidad de luz en la formación y multiplicación de embriones somáticos de soya cultivar INCASoy-27. Para ello, cotiledones inmaduros de 3,0-4,0 mm de longitud fueron colocados en medio de cultivo con 40 mg.l-1 de ácido 2,4-diclorofenoxiacético, sacarosa 3% y pH 7,0 en cámaras de crecimiento con luz artificial e intensidades luminosas de 5-10 µmol.m-2.s-1 y 68-73 µmol.m-2.s-1, y en cámaras de crecimiento con luz solar con 50-65µmol.m-2.s-1. El mayor número de cotiledones embriogénicos (95,31 se obtuvo en condiciones de luz artificial con alta intensidad luminosa. La mayor formación de embriones somáticos se obtuvo en luz artificial (9,65 y luz solar (8,45 respectivamente con alta intensidad luminosa. No se encontraron diferencias significativas en la multiplicación de los embriones somáticos, al emplear ambas fuente de luz con alta intensidad luminosa. Esta es la primera referencia sobre formación y multiplicación de embriones somáticos de soya con empleo de luz solar. Palabras clave: Cultivo de tejido; embriogénesis somática; fotoperíodo; Glycine max. Abstract: The somatic embryogenesis represents an alternative for the regeneration of plants, in which influence diverse factors during the cultivation. Among these factors, the conditions of illumination during the induction of somatic embryos are determinant. However, works not exist that refer the effect

  7. Effects of Combined Selective Aerobic Moderate Intensity Exercises and Soya Intake on Serum Lipids and Obesity in Obese Postmenopausal Women

    Directory of Open Access Journals (Sweden)

    A Zarneshan

    2012-02-01

    Full Text Available Background & Aim: Today, the percentage of deaths due to heart diseases has increased and along with the exercise training role in reducing obesity and cardiovascular disease, soy is a useful source of food in reducing blood lipid and obesity. The present study investigated the effect of combined selective short aerobic moderate intensity exercise and soya intake on serum lipids and obesity in obese postmenopausal women. Methods: In the present quasi-experimental study conducted in 2006 on women of Urmia, Iran, 56 obese postmenopausal women were selected and randomly divided into four groups of 14 subjects as follow: exercise-soya, exercise, soya, and control group. Pre- and post protocol blood samples were collected and the level of TG (Triglyceride, tCho (Total Cholesterol, LDL-C (Low density lipoprotein, HDL-C (high density lipoprotein were measured. Exercise training within the range of 60-70% maximum heart rate (MHR was performed for 60 minutes, 3 sessions per week. The subjects of soya group and exercise-soya group had a 100 gram soya nut intake daily for 10 weeks. After The training course, blood samples were taken from the subjects. The collected data was analyzed using Two-way ANOVA and paired t-test. Results: Having soya along with exercise had significant impact on reduction of TG, tCho, LDL-C means (p<0.05. Body mass index and waist to hip ratio decreased significantly in exercise-soya group after 10 weeks (p<0.05. Conclusion: Based on the findings of this study, aerobic moderate intensity exercise along with soya intake, decrease obesity and serum lipids in obese postmenopausal women.

  8. Effect of ultra high pressure homogenization treatment on the bioactive compounds of soya milk.

    Science.gov (United States)

    Toro-Funes, N; Bosch-Fusté, J; Veciana-Nogués, M T; Vidal-Carou, M C

    2014-01-01

    Ultra high pressure homogenization (UHPH) is a useful novel technology to obtain safe and high-quality liquid foods. The effect of UHPH at 200 and 300 MPa in combination with different inlet temperatures (Tin) (55, 65 and 75 °C) on the bioactive compounds of soya milk was studied. Total phytosterols increased with the higher combination of pressure and temperature. The main phytosterol was β-sitosterol, followed by stigmasterol and campesterol. Total tocopherols in UHPH-treated soya milks decreased as the temperature and pressure increased. UHPH treatment also affected the different chemical forms of tocopherols. No biogenic amines were detected in any of the analyzed soya milks. Meanwhile, the polyamines SPD and SPM were found in all soya milks, being stable to the UHPH treatment. Total isoflavones increased with the higher combination of pressure and temperature. No differences in the isoflavone profile were found, with β-glucoside conjugates being the predominant form. Copyright © 2013 Elsevier Ltd. All rights reserved.

  9. Biogas production enhancement by soya sludge amendment in cattle dung digesters

    Energy Technology Data Exchange (ETDEWEB)

    Satyanarayan, Shanta; Ramakant; Shivayogi [WWT Division, NEERI, Nagpur 400 020 (India)

    2010-09-15

    Biogas energy production from cattle dung is an economically feasible and eco-friendly in nature. But dependence only on cattle dung is a limiting factor. Rich nitrogen containing substrate addition to extra carbohydrate digester like cattle dung could improve the biogas production. Detailed performance of the digesters at different ratios of cattle dung and soya sludge has been discussed in this paper considering the cold countries climate. Soya sludge substrate not only has high nitrogen content of 4.0-4.8% but it also has high percentage of volatile solids content in the range of 97.8-98.8%. Soya sludge addition also improved the manurial value of the digested slurry and also improved the dewater-ability of the sludge. Results indicated an increment of 27.0% gas production at 25.0% amendment of soya sludge in non-homogenized cattle dung (NCD) digester. The amount of gas production increased to 46.4% in case of homogenized cattle dung (HCD) with respect to NCD feed at the same amendment. (author)

  10. Fermented soya bean (tempe) extracts reduce adhesion of enterotoxigenic Escherichia coli to intestinal epithelial cells

    NARCIS (Netherlands)

    Roubos-van den Hil, P.J.; Nout, M.J.R.; Beumer, R.R.; Meulen, van der J.; Zwietering, M.H.

    2009-01-01

    Aims: This study aimed to investigate the effect of processed soya bean, during the successive stages of tempe fermentation and different fermentation times, on adhesion of enterotoxigenic Escherichia coli (ETEC) K88 to intestinal brush border cells as well as Caco-2 intestinal epithelial cells; and

  11. Prenylated isoflavonoids from soya and licorice : analysis, induction and in vitro estrogenicity

    NARCIS (Netherlands)

    Simons, R.

    2011-01-01


    Prenylatedisoflavonoids are found in large amountsin soya bean (Glycine max) germinated under stress and in licorice (Glycyrrhiza glabra). Prenylation of isoflavonoids has been associated with modification of theirestrogenic activity.
    The aims of this thesis were (1) to provide a

  12. Natural occurrence of alternariol and alternariol monomethyl ether in soya beans.

    Science.gov (United States)

    Oviedo, M S; Barros, G G; Chulze, S N; Ramirez, M L

    2012-08-01

    The natural occurrence of alternariol (AOH) and alternariol monomethyl ether (AME) in soya beans harvested in Argentina was evaluated. Both toxins were simultaneously detected by using HPLC analysis coupled with a solid phase extraction column clean-up. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of recovery test with spiked soya bean samples. Out of 50 soya bean samples, 60% showed contamination with the mycotoxins analyzed; among them, 16% were only contaminated with AOH and 14% just with AME. Fifteen of the positive samples showed co-occurrence of both mycotoxins analyzed. AOH was detected in concentrations ranging from 25 to 211 ng/g, whereas AME was found in concentrations ranging from 62 to 1,153 ng/g. Although a limited number of samples were evaluated, this is the first report on the natural occurrence of Alternaria toxins in soya beans and is relevant from the point of view of animal public health.

  13. Elaboration and characterization of nanoliposome made of soya; rapeseed and salmon lecithins: application to cell culture.

    Science.gov (United States)

    Arab Tehrany, Elmira; Kahn, Cyril J F; Baravian, Christophe; Maherani, Behnoush; Belhaj, Nabila; Wang, Xiong; Linder, Michel

    2012-06-15

    Health benefits of unsaturated fatty acids have been demonstrated over the last decades. Nanotechnology provided new process to produce particles such as liposomes and nanoliposomes made of pure phospholipids. These techniques are already used in pharmaceutics to augment the bioavailability and the bioefficiency of drugs. The aim of this paper is to characterize and evaluate the potential of nanoliposomes made of three lecithins (soya, rapeseed and salmon) on cell culture in order to use them in the future as drug delivery systems for tissue engineering. We began to measure, with zetasizer, the radius size of liposomes particles which are 125.5, 136.7 and 130.3 nm respectively for rapeseed, soya and salmon lecithin. Simultaneously, solutions observed by TEM demonstrated the particles were made much of liposomes than droplet (emulsion). Finally, we found that the solutions of lecithins were enough stable over 5 days at 37 °C to be used in culture medium. We investigated the effect of soya, rapeseed and salmon lecithin liposome from 2mg/mL to 5.2 μg/mL on metabolic activity and cell proliferation on rat bone marrow stem cells (rBMSC) during 14 days. The results showed that the three lecithins (soya, rapeseed and salmon) improve cell proliferation at different concentration. Copyright © 2012 Elsevier B.V. All rights reserved.

  14. Production of peptone from soya beans (Glycine max L merr) and ...

    African Journals Online (AJOL)

    AJB SERVER

    2006-09-18

    Sep 18, 2006 ... Peptone was produced from soya beans and African locust beans. The produced peptones were evaluated as component of microbiological media for the growth of some bacteria and compared with some commercial peptones. Some of the tested bacteria are Escherichia coli, Pseudomonas aeruginosa ...

  15. In vitro digestibility of processed and fermented soya bean, cowpea and maize

    NARCIS (Netherlands)

    Kiers, J.L.; Nout, M.J.R.; Rombouts, F.M.

    2000-01-01

    Tropical legumes, ie soya bean and cowpea, were pre-treated and subsequently fermented using pure cultures of Rhizopus spp. Impact of soaking, cooking and fermentation of the legumes on their digestibility was determined using an in vitro digestion method. Processing of white maize included, amongst

  16. Fermentation characteristics of polysaccharide fractions extracted from the cell walls of soya bean cotyledons

    NARCIS (Netherlands)

    Laar, van H.; Tamminga, S.; Williams, B.A.; Verstegen, M.W.A.; Schols, H.A.

    2000-01-01

    Full-fat soya beans were separated into hulls and cotyledons. After separation the cell wall fraction was extracted from the cotyledons. These purified cell walls were sequentially extracted with 0.05 M cyclohexane-trans-1,2-diamine-N,N,N ,N -tetraacetate (CDTA) 0.05 M NH4 oxalate (extract 1), 0.05

  17. “Eat dry beans, split peas, lentils and soya regularly”: A food-based ...

    African Journals Online (AJOL)

    Eat dry beans, split peas, lentils and soya regularly”: A food-based dietary guideline. ... Legumes are rich and economical sources of good-quality protein, slow-release carbohydrates, dietary fibre (non-starch polysaccharides), various vitamins and minerals and non-nutritive components which may have several beneficial ...

  18. The Effect of Soya bean ( Glycine max ) on Pefloxacin Absorption in ...

    African Journals Online (AJOL)

    The study was to investigate the effect of soya bean on the absorption of pefloxacin when given by oral route in rats. The first group of animals feeding on standard pellet feeds was given pefloxacin (8 mg/kg, p.o), while the second and third groups were also given the drug at the same dosage level but were fed with 50 ...

  19. Evaluation of processed sweet potato-crayfish – soya bean and ...

    African Journals Online (AJOL)

    The results obtained with diet 3 (sweat potato bambara groundnut mixture) and diet 5 (sweat potato soya bean mixture) compared favourably with the reference diet (Nutrend) in all the parameters examined except for corn starch (diet 7), which recorded lowest values although it contained higher carbohydrate and energy ...

  20. Monoclonal antibodies to the major Lolium perenne (rye grass) pollen allergen Lol p I (Rye I).

    Science.gov (United States)

    Kahn, C R; Marsh, D G

    1986-12-01

    Thirteen monoclonal antibodies (MAbs) were produced against Lol p I (Rye I), the major Lolium perenne (rye grass) pollen allergen. Spleen cells from A/J and SJL mice immunized with highly purified Lol p I (Lol I) were allowed to fuse with cells from the non-secreting Sp2/0-Ag14 myeloma cell line. Each MAb was analyzed for antigenic specificity by radioimmunoassay (RIA) using 125I-Lol I. The epitope specificities of seven of the MAbs were examined by competitive binding against a labelled standard MAb for the Lol I antigen (Ag). The dissociation constant, Kd, of one MAb (No. 3.2) that was studied most extensively was determined by double Ab RIA to be 3.5 X 10(-6) L/M. This MAb recognized the related 27,000-30,000 Group I glycoproteins found in the pollens of nine other species of grass pollens tested, including weak binding to Bermuda grass Group I (Cyn d I), which by conventional analysis using polyclonal anti-Lol I serum shows no detectable binding. Monoclonal antibody No. 3.2 was coupled covalently to Sepharose 4B and used to prepare highly purified Lol I from a partially purified rye pollen extract. Finally, an RIA was developed which permitted the analysis of the Group I components in rye grass and nine other grass pollen species. The latter assay is likely to prove useful in the standardization of grass pollen extracts according to their Group I contents.

  1. Complete amino acid sequence of a Lolium perenne (perennial rye grass) pollen allergen, Lol p II.

    Science.gov (United States)

    Ansari, A A; Shenbagamurthi, P; Marsh, D G

    1989-07-05

    The complete amino acid sequence of a Lolium perenne (rye grass) pollen allergen, Lol p II was determined by automated Edman degradation of the protein and selected fragments. Cleavage of the protein by enzymatic and chemical techniques established an unambiguous sequence for the protein. Lol p II contains 97 amino acid residues, with a calculated molecular weight of 10,882. The protein lacks cysteine and glutamine and shows no evidence of glycosylation. Theoretical predictions by Fraga's (Fraga, S. (1982) Can. J. Chem. 60, 2606-2610) and Hopp and Woods' (Hopp, T. P., and Woods, K. R. (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 3824-3828) methods indicate the presence of four hydrophilic regions, which may contribute to sequential or parts of conformational B-cell epitopes. Analysis of amphipathic regions by Berzofsky's method indicates the presence of a highly amphipathic region, which may contain, or contribute to, an Ia/T-cell epitope. This latter segment of Lol p II was found to be highly homologous with an antibody-binding segment of the major rye allergen Lol p I and may explain why immune responsiveness to both the allergens is associated with HLA-DR3.

  2. Toxicity and uptake of cyclic nitramine explosives in ryegrass Lolium perenne

    International Nuclear Information System (INIS)

    Rocheleau, Sylvie; Lachance, Bernard; Kuperman, Roman G.; Hawari, Jalal; Thiboutot, Sonia; Ampleman, Guy; Sunahara, Geoffrey I.

    2008-01-01

    Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) are cyclic nitramines used as explosives. Their ecotoxicities have been characterized incompletely and little is known about their accumulation potential in soil organisms. We assessed the toxicity and uptake of these explosives in perennial ryegrass Lolium perenne L. exposed in a Sassafras sandy loam (SSL) or in a sandy soil (DRDC, CL-20 only) containing contrasting clay contents (11% and 0.3%, respectively). A 21-d exposure to RDX, HMX or CL-20 in either soil had no adverse effects on ryegrass growth. RDX and HMX were translocated to ryegrass shoots, with bioconcentration factors (BCF) of up to 15 and 11, respectively. In contrast, CL-20 was taken up by the roots (BCF up to 19) with no translocation to the shoots. These studies showed that RDX, HMX, and CL-20 can accumulate in plants and may potentially pose a risk of biomagnification across the food chain. - Cyclic nitramine explosives accumulate in perennial ryegrass and exhibit distinct uptake patterns

  3. Genetic Loci Governing Androgenic Capacity in Perennial Ryegrass (Lolium perenne L.

    Directory of Open Access Journals (Sweden)

    Rachel F. Begheyn

    2018-06-01

    Full Text Available Immature pollen can be induced to switch developmental pathways from gametogenesis to embryogenesis and subsequently regenerate into homozygous, diploid plants. Such androgenic production of doubled haploids is particularly useful for species where inbreeding is hampered by effective self-incompatibility systems. Therefore, increasing the generally low androgenic capacity of perennial ryegrass (Lolium perenne L. germplasm would enable the efficient production of homozygous plant material, so that a more effective exploitation of heterosis through hybrid breeding schemes can be realized. Here, we present the results of a genome-wide association study in a heterozygous, multiparental population of perennial ryegrass (n = 391 segregating for androgenic capacity. Genotyping-by-sequencing was used to interrogate gene- dense genomic regions and revealed over 1,100 polymorphic sites. Between one and 10 quantitative trait loci (QTL were identified for anther response, embryo and total plant production, green and albino plant production and regeneration. Most traits were under polygenic control, although a major QTL on linkage group 5 was associated with green plant regeneration. Distinct genetic factors seem to affect green and albino plant recovery. Two intriguing candidate genes, encoding chromatin binding domains of the developmental phase transition regulator, Polycomb Repressive Complex 2, were identified. Our results shed the first light on the molecular mechanisms behind perennial ryegrass microspore embryogenesis and enable marker-assisted introgression of androgenic capacity into recalcitrant germplasm of this forage crop of global significance.

  4. Toxicity and uptake of cyclic nitramine explosives in ryegrass Lolium perenne

    Energy Technology Data Exchange (ETDEWEB)

    Rocheleau, Sylvie; Lachance, Bernard [Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2 (Canada); Kuperman, Roman G. [Edgewood Chemical Biological Center, 5183 Blackhawk Road, Aberdeen Proving Ground, MD 21010-5424 (United States); Hawari, Jalal [Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2 (Canada); Thiboutot, Sonia; Ampleman, Guy [Defense Research and Development Canada, 2459 Pie IX Boulevard, Val Belair, Quebec G3J 1X5 (Canada); Sunahara, Geoffrey I. [Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2 (Canada)], E-mail: geoffrey.sunahara@cnrc-nrc.gc.ca

    2008-11-15

    Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) are cyclic nitramines used as explosives. Their ecotoxicities have been characterized incompletely and little is known about their accumulation potential in soil organisms. We assessed the toxicity and uptake of these explosives in perennial ryegrass Lolium perenne L. exposed in a Sassafras sandy loam (SSL) or in a sandy soil (DRDC, CL-20 only) containing contrasting clay contents (11% and 0.3%, respectively). A 21-d exposure to RDX, HMX or CL-20 in either soil had no adverse effects on ryegrass growth. RDX and HMX were translocated to ryegrass shoots, with bioconcentration factors (BCF) of up to 15 and 11, respectively. In contrast, CL-20 was taken up by the roots (BCF up to 19) with no translocation to the shoots. These studies showed that RDX, HMX, and CL-20 can accumulate in plants and may potentially pose a risk of biomagnification across the food chain. - Cyclic nitramine explosives accumulate in perennial ryegrass and exhibit distinct uptake patterns.

  5. Efecto del glifosato sobre el crecimiento y acumulación de azúcares libres en dos biotipos de lolium perenne de distinta sensibilidad al herbicida Glyphosate effects on the growth and free sugar accumulation of two lolium perenn e biotypes with different herbicide sensitivity

    Directory of Open Access Journals (Sweden)

    M. Yanniccari

    2012-03-01

    Full Text Available El movimiento sistémico del glifosato está determinado por el transporte de fotoasimilados. A su vez, la capacidad de un destino de consumir los asimilados está condicionada por su actividad metabólica. Pese a su importancia, la relación entre el glifosato y la síntesis de azúcares en hojas fuente ha sido poco abordada. El objetivo del presente trabajo fue evaluar los efectos del glifosato sobre el crecimiento y la acumulación de azúcares libres en dos biotipos de Lolium perenne de baja y alta sensibilidad al herbicida. Se trabajó con clones de ambos tipos de plantas, en macollaje, tratados con 1.440 g e.a. ha-1 de glifosato y sin tratamiento herbicida como controles. Se evaluó periódicamente el efecto del glifosato sobre el rebrote de hojas hasta las 50 horas post-aplicación y sobre los niveles de azúcares libres totales, reductores y no reductores en hojas a 1, 2, 3 y 5 días post-aplicación. A partir de las 25 horas post-aplicación, el glifosato provocó una disminución del crecimiento del 58% en el biotipo susceptible, con una acumulación de azúcares libres superior al 90% con relación al control, desde el primer día post-aplicación en adelante. La inhibición del crecimiento, inducida por el glifosato en plantas susceptibles, no depende de la limitación del traslado de fotoasimilados desde la parte aérea. Por tanto, la acumulación de azúcares libres en hojas podría explicarse por la caída en la tasa de crecimiento. En el biotipo de baja sensibilidad, en el que no se detectó inhibición del crecimiento, estos efectos fueron limitados.The systemic movement of glyphosate is determined by the transport of photoassimilates. In turn, the capacity of a destination to consume assimilates is conditioned by their metabolic activity. Despite its importance, the relationship between the glyphosate and the sugar synthesis from source leaves has been little studied. The aim of this work was to determine the effect of

  6. Use of Bacillus subtilis isolates from Tua-nao towards nutritional improvement of soya bean hull for monogastric feed application.

    Science.gov (United States)

    Wongputtisin, P; Khanongnuch, C; Kongbuntad, W; Niamsup, P; Lumyong, S; Sarkar, P K

    2014-09-01

    Soya bean hull (SBH) is a cheap and high-fibre content feed ingredient that obtained after soya bean oil extraction. Microbial fermentation was expected to improve SBH qualities before applying to animals, especially monogastric animals. Two bacterial strains, Bacillus subtilis MR10 and TK8 that were isolated from Tua-nao, a traditional fermented soya bean in northern Thailand, were used for fermented soya bean hull (FSBH) production. Both could easily grow at 37°C in SBH as the sole substrate. MR10 produced the highest β-mannanase activity (400 U g(-1) SBH) on day 2, while TK8 produced the highest cellulase activity (14·5 U g(-1) SBH) on day 3. After fermentation, the nutritional quality of SBH was obviously improved by an increase in soluble sugars, soluble proteins, crude protein and crude lipid, and a decrease in the content of raffinose family oligosaccharides. Scavenging activity (%) of SBH against ABTS radical cation was also increased from 14 to 27 and 20% by MR10 and TK8 fermentation, respectively. According to the GRAS property of these both strains and various improvements of nutritional values, the fermented SBH proved to be a potential feed ingredient, especially for the monogastric animals. Normally, soya bean hull has been recognized as only a worthless by-product from soya bean oil production process because of its low utilizable nutrients. Our study introduced an alternative way to utilize this worthless residue using biotechnological knowledge. The nutritional quality of soya bean hull was improved by microbial fermentation. Fermented soya bean hull can be used as a cheap, safe and high-nutrient feed ingredient for livestock production, especially monogastric animals, to promote their growth performances, instead of using antibiotics in some regions of the world. © 2014 The Society for Applied Microbiology.

  7. Efecto de la densidad de población y la fertilización en el cultivo de soya (Glycine max (L Merr de doble producción (forraje y grano

    Directory of Open Access Journals (Sweden)

    Forero P. Jaime A.

    1986-03-01

    Full Text Available En el estudio se utilizó un diseño de parcelas divididas, correspondiendo la fertilización a las parcelas principales y a las sub parcelas las tres densidades de población. La soya puede utilizarse como planta de doble fin. Con una población de 360000 plantas/ha con fertilización se logra la mejor producción de forraje en materia seca (865.44 kg/ha y la máxima producción de grano (1073 kq/ha. Se considera que este es el primer trabajo que se realiza en Colombia en donde se utiliza la soya como planta forrajera y a la vez productora de grano en condiciones de campo.The possibility of developing a "double production" (green forage and grain system, the influence of population densities and fertilization on soybean were studied. A design of divided plots was used (main plots: fertilization and subplots: three population densities l. The soybean can be used as a double purpose plant. A population density of 360000 plants/ha with fertilization gives the best forage yield in dry matter (865.44 kg/ha and the maximum grain production (1073 kq/ha. This work is considered to be the first done in Colombia using soybean as forage and grain producer under field conditions.

  8. Solid state fermentation with lactic acid bacteria to improve the nutritional quality of lupin and soya bean.

    Science.gov (United States)

    Bartkiene, Elena; Krungleviciute, Vita; Juodeikiene, Grazina; Vidmantiene, Daiva; Maknickiene, Zita

    2015-04-01

    The ability of bacteriocin-like inhibitory substance (BLIS)-producing lactic acid bacteria (LAB) to degrade biogenic amines as well as to produce L(+) and D(-)-lactic acid during solid state fermentation (SSF) of lupin and soya bean was investigated. In addition, the protein digestibility and formation of organic acids during SSF of legume were investigated. Protein digestibility of fermented lupin and soya bean was found higher on average by 18.3% and 15.9%, respectively, compared to untreated samples. Tested LAB produced mainly L-lactic acid in soya bean and lupin (D/L ratio 0.38-0.42 and 0.35-0.54, respectively), while spontaneous fermentation gave almost equal amounts of both lactic acid isomers (D/L ratio 0.82-0.98 and 0.92, respectively). Tested LAB strains were able to degrade phenylethylamine, spermine and spermidine, whereas they were able to produce putrescine, histamine and tyramine. SSF improved lupin and soya bean protein digestibility. BLIS-producing LAB in lupin and soya bean medium produced a mixture of D- and L-lactic acid with a major excess of the latter isomer. Most toxic histamine and tyramine in fermented lupin and soya bean were found at levels lower those causing adverse health effects. Selection of biogenic amines non-producing bacteria is essential in the food industry to avoid the risk of amine formation. © 2014 Society of Chemical Industry.

  9. Engineering soya bean seeds as a scalable platform to produce cyanovirin-N, a non-ARV microbicide against HIV.

    Science.gov (United States)

    O'Keefe, Barry R; Murad, André M; Vianna, Giovanni R; Ramessar, Koreen; Saucedo, Carrie J; Wilson, Jennifer; Buckheit, Karen W; da Cunha, Nicolau B; Araújo, Ana Claudia G; Lacorte, Cristiano C; Madeira, Luisa; McMahon, James B; Rech, Elibio L

    2015-09-01

    There is an urgent need to provide effective anti-HIV microbicides to resource-poor areas worldwide. Some of the most promising microbicide candidates are biotherapeutics targeting viral entry. To provide biotherapeutics to poorer areas, it is vital to reduce the cost. Here, we report the production of biologically active recombinant cyanovirin-N (rCV-N), an antiviral protein, in genetically engineered soya bean seeds. Pure, biologically active rCV-N was isolated with a yield of 350 μg/g of dry seed weight. The observed amino acid sequence of rCV-N matched the expected sequence of native CV-N, as did the mass of rCV-N (11 009 Da). Purified rCV-N from soya is active in anti-HIV assays with an EC50 of 0.82-2.7 nM (compared to 0.45-1.8 nM for E. coli-produced CV-N). Standard industrial processing of soya bean seeds to harvest soya bean oil does not diminish the antiviral activity of recovered rCV-N, allowing the use of industrial soya bean processing to generate both soya bean oil and a recombinant protein for anti-HIV microbicide development. © 2015 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  10. Standardized ileal digestibility of amino acids in European soya bean and rapeseed products fed to growing pigs.

    Science.gov (United States)

    Kaewtapee, C; Mosenthin, R; Nenning, S; Wiltafsky, M; Schäffler, M; Eklund, M; Rosenfelder-Kuon, P

    2018-04-01

    This study was conducted to determine the chemical composition and standardized ileal digestibility coefficients (SID) of crude protein (CP) and amino acids (AA) of European soya bean and rapeseed products in pigs. Six soya bean and two rapeseed products were used as the sole dietary source of CP and AA, including raw (FFSB) and roasted full-fat soya beans (FFSB R oasted ), soya bean (SBC) and rapeseed cake (RSC), and rapeseed meal (RSM) from Bavaria (Germany), soya bean meal (SBM) from the Danube region (Austria; SBM A ustria ), a commercially available standard SBM (SBM S td ) and an imported genetically modified organism-free SBM (SBM GMO -free ). Eight ileal- cannulated pigs with an initial body weight of 32 ± 2 kg were allotted to a row-column design with eight diets and six periods of seven days each. Trypsin inhibitor activity (TIA) ranged from 1.8 in SBM S td to 24.5 mg/g DM in FFSB. The SID of CP and all AA in FFSB R oasted were greater than in FFSB, but lower when compared to SBC and SBM A ustria (p soya bean and rapeseed products as influenced by differences in processing conditions. European SBC and SBM A ustria can be used as alternative to imported SBM GMO -free and SBM S td in diets for growing pigs. © 2017 Blackwell Verlag GmbH.

  11. 40 CFR 721.10055 - 1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-soya acyl derivs., inner salts.

    Science.gov (United States)

    2010-07-01

    ...-(carboxymethyl)-N,N-dimethyl-, N-soya acyl derivs., inner salts. 721.10055 Section 721.10055 Protection of...-amino-N-(carboxymethyl)-N,N-dimethyl-, N-soya acyl derivs., inner salts. (a) Chemical substance and...-(carboxymethyl)-N,N-dimethyl-, N-soya acyl derivs., inner salts (PMN P-03-46; CAS No. 136504-87-5) is subject to...

  12. Evaluación de algunos factores que inciden en la perdida de germinación de la semilla de soya

    Directory of Open Access Journals (Sweden)

    Gómez Carlos

    1990-06-01

    Full Text Available En el Centro de Investigación del lCA en Palmira se realizó un estudio durante 1989, con el fin de evaluar algunos factores que inciden en la pérdida del vigor y germinación de la semilla de soya (Glycine max; se observó la incidencia de la época de cosecha y el manejo en los diferentes períodos de almacenamiento. La semilla de soya provino de dos fuentes: la primera se obtuvo de campos del Cl Palmira perteneciente a semilla básica de las variedades SOYlCA P - 33, SOYlCA P -32, lCA TUNlA y ARlARI - 1 con las cuales se evaluaron cinco épocas de cosecha. La segunda fuente provino de cuatro casas productoras, dos variedades (SOYICA P-33 e ICA TUNIA con dos sistemas de beneficio (acondicionada y sin acondicionar evaluándose los efectos de los daños mecánicos en el almacenamiento. La época óptima para cosecha de la semilla con la más alta calidad fisiológica se obtuvo en el segundo período de cosecha a los 112 días después de la siembra, y la variedad que mejor comportamiento presentó fue lCA TUNIA. En las semillas no acondicionadas se encontró mejor comportamiento en la variedad ICA TUNIA. Además, presentó mejor germinación.A study was carried out during 1989 in the Centro Nacional de Investigación Palmira with the objective to search the incidence of factors in the lost of vigor and germination of soybean seed during several harvesting time and conditioning. The first experiment consisted of collecting seed from physiological maturity up to 28 days after, using the varieties SOYICA p. 33, SOYICA P-32, ICA TUNIA and SOYICA ARIARI-1; the information included 5 harvesting times (one each week. The second experiment included seeds conditioned and non conditioned of the varieties SOYICA P-33 and ICA TUNIA from the commercial seed companies. The research concluded that the second harvest after physiological maturity was the best for seed quality and the quality decreased with the time of harvest; also, the variety SOYICA P-33

  13. Immunochemical studies of Lolium perenne (rye grass) pollen allergens, Lol p I, II, and III.

    Science.gov (United States)

    Ansari, A A; Kihara, T K; Marsh, D G

    1987-12-15

    It was reported earlier that human immune responses to three perennial rye grass (Lolium perenne) pollen allergens, Lol p I, II, and III, are associated with histocompatibility leukocyte antigen (HLA)-DR3. Rye-allergic people are often concordantly sensitive to all three of these allergens. Since earlier studies suggested that these antigens are non-cross-reactive, their immunologic relatedness by double antibody radioimmunoassay (DARIA) was studied in order to understand further the immunochemical basis for the concordant recognition of the three allergens. Direct binding DARIA studies were performed with human sera from 189 allergic subjects. Inhibition DARIA studies were carried out with 17 human sera from grass-allergic patients who were on grass immunotherapy, one goat anti-serum, and six rabbit antisera. None of the sera detected any significant degree of two-way cross-reactivity between Lol p I and II, or between Lol p I and III. However, the degree of two-way cross-reactivity between Lol p II and III exhibited by individual human and animal antisera varied between undetectable and 100%. In general, the degree of cross-reactivity between Lol p II and III was higher among human sera than among animal sera. Taken together with earlier findings that antibody responses to Lol p I, II and III are associated with HLA-HDR3, and that most Lol p II and III responders are also Lol p I responders, but not vice versa, our present results suggest the following: the HLA-DR3-encoded Ia molecule recognizes a similar immunodominant Ia recognition site (agretope) shared between Lol p I and Lol p II and/or III; in addition, Lol p I appears to contain unique Ia recognition site(s) not present in Lol p II and III. However, further epitope analyses are required to investigate these possibilities.

  14. Molecular genetics of human immune responsiveness to Lolium perenne (rye) allergen, Lol p III.

    Science.gov (United States)

    Ansari, A A; Freidhoff, L R; Marsh, D G

    1989-01-01

    Lol p II and III are each about 11-kD protein allergens from the pollen of Lolium perenne (rye grass). We have found that human immune responses (IgE and IgG antibodies) to both proteins are significantly associated with HLA-DR3. In addition, the two proteins are cross-reactive with the antibodies in many human sera (about 84% human sera showed the cross-reactivity). We have determined greater than 90% of the amino acid sequences of the two proteins and found that they are at least 54% homologous. Berzofsky found that 75% of the 23 known T cell sites in various proteins had an amphipathic structure. Our analysis by the same method showed that both Lol p II and III have a major region of amphipathicity (at residues 61-67, Lol p III numbering) which might contain sites for binding to an Ia molecule and a T cell receptor. This region is identical between Lol p II and III, except for an Arg-Lys substitution, and could account, in part, for the DR3 association with responsiveness to both molecules. An interesting difference between the two proteins is that immune response to Lol p III is associated with DR5 (in addition to DR3), whereas no DR5 association is found in the case of Lol p II. One possibility is that Lol p III has an additional site which binds to the DR5 Ia molecule. Lol p III indeed has a second highly amphiphathic peptide, 24-30 (Lol p III 24 R P G D T L A 30), which is different and not amphipathic in Lol p II.(ABSTRACT TRUNCATED AT 250 WORDS)

  15. Recycling of biological sludge for the fertilizing of soils cultivated with Lolium perenne

    Directory of Open Access Journals (Sweden)

    Florica Morariu

    2017-05-01

    Full Text Available The present study has been elaborated with the aim of justifying the high efficiency of in-situ slaughterhouse sludge recycling and its usage in Lolium perenne cultures. Stabilized slaughterhouse sludge was used to complete the high deficiency in nutrients of the poor terrains. Slaughterhouse sludge represents an excessive, final by product from a meat-processing unit in Western Romania. It contains 59.78-90.77% easily bio-degradable organic substances. Moreover, it has compounds containing nitrogen and phosphorus, total N=1.922-3.318%, total P=1107-1126mg•kg-1D.M. The experimental variants have been prepared, having the following characteristics: control variants of non-fertilized soils and variants of soils fertilized with slaughterhouse sludge, 50t•ha-1. The experimental variants used were arranged in a completely randomized block design, with three replicates each. The efficiency of fertilization with slaughterhouse sludge was a 30-35% rise in the quantity of grass harvested vs. the quantities harvested from the control variants. The quantity of Cd and Pb has been determined from the aerial parts of the harvested plants and they were below the maximum limit admitted by the sanitary regulations in Romania. Cr didn’t bio-accumulate in plants at a detection limit. The quantity of other metals determined from the aerial parts of the plants was low: i.e. <10mg•kg-1D.M. for Cu or Ni <50mg•kg-1D.M. for Zn, <150mg•kg-1D.M.for Mn. Green feed harvested from fields fertilized with slaughterhouse sludge can be part of animal nutrition.

  16. Plants modify biological processes to ensure survival following carbon depletion: a Lolium perenne model.

    Directory of Open Access Journals (Sweden)

    Julia M Lee

    Full Text Available BACKGROUND: Plants, due to their immobility, have evolved mechanisms allowing them to adapt to multiple environmental and management conditions. Short-term undesirable conditions (e.g. moisture deficit, cold temperatures generally reduce photosynthetic carbon supply while increasing soluble carbohydrate accumulation. It is not known, however, what strategies plants may use in the long-term to adapt to situations resulting in net carbon depletion (i.e. reduced photosynthetic carbon supply and carbohydrate accumulation. In addition, many transcriptomic experiments have typically been undertaken under laboratory conditions; therefore, long-term acclimation strategies that plants use in natural environments are not well understood. METHODOLOGY/PRINCIPAL FINDINGS: Perennial ryegrass (Lolium perenne L. was used as a model plant to define whether plants adapt to repetitive carbon depletion and to further elucidate their long-term acclimation mechanisms. Transcriptome changes in both lamina and stubble tissues of field-grown plants with depleted carbon reserves were characterised using reverse transcription-quantitative polymerase chain reaction (RT-qPCR. The RT-qPCR data for select key genes indicated that plants reduced fructan degradation, and increased photosynthesis and fructan synthesis capacities following carbon depletion. This acclimatory response was not sufficient to prevent a reduction (P<0.001 in net biomass accumulation, but ensured that the plant survived. CONCLUSIONS: Adaptations of plants with depleted carbon reserves resulted in reduced post-defoliation carbon mobilization and earlier replenishment of carbon reserves, thereby ensuring survival and continued growth. These findings will help pave the way to improve plant biomass production, for either grazing livestock or biofuel purposes.

  17. ‘‘Blind'' mapping of genic DNA sequence polymorphisms in Lolium perenne L. by high resolution melting curve analysis

    DEFF Research Database (Denmark)

    Studer, Bruno; Jensen, Louise Bach; Fiil, Alice

    2009-01-01

    High resolution melting curve analysis (HRM) measures dissociation of double stranded DNA of a PCR product amplified in the presence of a saturating fluorescence dye. Recently, HRM proved successful to genotype DNA sequence polymorphisms such as SSRs and SNPs based on the shape of the melting...... curves. In this study, HRM was used for simultaneous screening and genotyping of genic DNA sequence polymorphisms identified in the Lolium perenne F2 mapping population VrnA. Melting profiles of PCR products amplified from previously published gene loci and from a novel gene putatively involved...

  18. Genetic and Environmental Variance Among F2 Families in a Commercial Breeding Program for Perennial Ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Fé, Dario; Greve-Pedersen, Morten; Jensen, Christian Sig

    2013-01-01

    In the joint project “FORAGESELECT”, we aim to implement Genome Wide Selection (GWS) in breeding of perennial ryegrass (Lolium perenne L.), in order to increase genetic response in important agronomic traits such as yield, seed production, stress tolerance and disease resistance, while decreasing...... of this study was to estimate the genetic and environmental variance in the training set composed of F2 families selected from a ten year breeding period. Variance components were estimated on 1193 of those families, sown in 2001, 2003 and 2005 in five locations around Europe. Families were tested together...

  19. Diseño de proceso para el desarrollo de barras energéticas como subproducto en la obtención de leche saborizada de soya

    OpenAIRE

    Gamboa Valarezo, Vannia; Costa, Ana Maria

    2009-01-01

    El grano de soya es ampliamente reconocido por su elevado contenido en nutrientes. Durante el proceso de elaboración de la leche de soya el okara o pulpa residual del proceso representó del 20 a 25 por ciento del desperdicio cuyo alto valor nutricional y energético fue aprovechado. En el presente trabajo se propuso aprovechar integralmente el grano de soya, dando valor agregado a la fase líquida donde se obtuvo leche saborizada de soya y, a partir del desperdicio se obtuvieron barras ene...

  20. Soya oil-based shampoo superior to 0.5% permethrin lotion for head louse infestation.

    Science.gov (United States)

    Burgess, Ian F; Kay, Katrina; Burgess, Nazma A; Brunton, Elizabeth R

    2011-01-01

    This was a randomized, assessor-blind, controlled comparison of a soya oil- based medical device shampoo with a medicinal permethrin lotion in an alcohol vehicle for treatment of head louse infestation to generate data suitable for a regulatory submission to achieve reimbursable status for the shampoo product. We treated 91 children and adults, divided between two sites, on two occasions 9 days apart. Participants washed their hair and towel-dried it before treatment. The shampoo was used twice for 30 minutes each time. The lotion was used for 30 minutes followed by rinsing. Assessments were made by dry detection combing on days 2, 9, 11, and 14 after the first treatment. According to present knowledge, this combing technique does not influence the overall head louse populations or outcome of treatment. The soya oil shampoo was significantly (P shampoo was more effective than the permethrin lotion, more cosmetically acceptable, and less irritant.

  1. Stability of tetrachlorvinphos residues in faba beans and soya bean oil towards different processing procedures

    International Nuclear Information System (INIS)

    Zayed, S.M.A.D.; Farghaly, M.

    1987-01-01

    Cooking of contaminated faba beans did not degrade the originally present potentially toxic residues, namely, tetrachlorvinphos and its desmethyl derivative to any appreciable extent. Processing of contaminated soya bean oil, on the other hand, led to degradation of tetrachlorvinphos and its metabolites to give mono and dimethyl phosphates. Feeding of mice with bound residues of tetrachlorvinphos in beans for 90 days led to an apparent decrease in the rate of body weight gain. (author)

  2. Scientists Grow Therapeutic Protein in Engineered Soya Bean Seeds to Prevent AIDS | Poster

    Science.gov (United States)

    Genetically modified soya beans provide a scalable, low-cost method of producing microbicides that prevent AIDS, a technique sustainable for resource-poor countries where AIDS is spreading rapidly. According to the Joint United Nations Programme on HIV/AIDS, more than 36 million people worldwide are living with HIV. While the number of AIDS-related deaths are decreasing, infection rates are still increasing, specifically in Eastern and Southern Africa.

  3. Analysis of peanuts and soya irradiated: is it possible to identify them?

    International Nuclear Information System (INIS)

    Leal, Alexandre S.; Vieira, Ionara F.R.; Tambourgi, Elias B.; Krambrock, Klaus; Prado, Guilherme

    2005-01-01

    In this work we present the results of the electronic paramagnetic resonance (EPR) analysis of peanuts and soya treated by gamma-irradiation. The dose employed varying from 1 to 30 kGy. The results obtained confirm that the EPR method is efficient to detect if the products were irradiated. In general, the EPR signal intensity increases according to the dose until saturation is reached making it possible to determine the dose level. (author)

  4. Underdeveloped spot markets and futures trading: The Soya Oil exchange in India

    OpenAIRE

    Bharat Ramaswami; Jatinder Bir Singh

    2006-01-01

    Abstract The limited presence of futures exchanges in developing countries where commodity markets fall short of the ideal underscore the importance of understanding the relation between spot and futures markets. The paper examines the exceptional success of the soya oil contract at the National Board of Trade (NBOT) in India. The paper asks whether the NBOT contract exhibits the fundamental features of mature futures markets in terms of its use by hedgers. If the market offers arbitrage oppo...

  5. Perennial grasses traits as functional markers of grazing intensity in basaltic grasslands of Uruguay Rasgos de gramíneas perennes como marcadores funcionales de la intensidad de pastoreo en pastizales de basalto en Uruguay

    Directory of Open Access Journals (Sweden)

    Martin Jaurena

    2012-12-01

    los grupos funcionales se determinó la respuesta al incremento de la intensidad de pastoreo en un experimento con dos cargas de capones (0,78 and 1,56 unidades ganaderas ha-1 cuantificando la cobertura de dichas especies y los valores de sus rasgos. El contenido de MS y el área foliar específica fueron los rasgos que mejor describieron la respuesta de las gramíneas perennes al incremento de la carga animal y podrían ser utilizados como marcadores funcionales. Tres grupos funcionales fueron identificados. El nivel más bajo de carga animal se relacionó con el grupo funcional A (especies estivales, altas, con baja área foliar específica y alto contenido de MS y con el grupo funcional B (especies invernales altas y con niveles intermedios de rasgos foliares. Por otro lado, el nivel más elevado de carga animal se relacionó con el aumento del grupo funcional C (especies estivales, postradas, con alta área foliar específica y bajos contenidos de MS. Esta clasificación de una comunidad con alta diversidad de especies en tres grupos funcionales es un nuevo enfoque para desarrollar métodos simples y generales para el diagnóstico del estado de praderas naturales de la región basáltica en Uruguay y generar recomendaciones para su manejo.

  6. Los costos de alimentación en relación con los costos totales en establecimientos típicos de tambo en países del Mercosur

    OpenAIRE

    Ostrowski, Bernardo

    2003-01-01

    p.1-10 Se analizan doce modelos de tambos típicos en la región MERCOSUR, desagregando los costos de alimentación en pastoreo directo, reservas forrajeras y concentrados, diferenciando entre verdeos anuales y pasturas perennes para el primero de los rubros especificados. Se relacionan los costos de la alimentación con los niveles de productividad media de los rodeos, con la escala de la empresa, con diferentes tecnologías y con los costos de los factores de producción intervinientes en la p...

  7. Isoflavones from green vegetable soya beans and their antimicrobial and antioxidant activities.

    Science.gov (United States)

    Wang, Taoyun; Liu, Yanli; Li, Xiaoran; Xu, Qiongming; Feng, Yulin; Yang, Shilin

    2018-03-01

    Green vegetable soya beans, known as Maodou in China, are supplied as vegetable-type fruits of the soybean plant. Previous study indicated that green vegetable soya beans exhibited antioxidative and anti-inflammatory activities. However, the material basis and pharmacological activities of green soybean plant were not unravelled clearly. In this study, we investigated the chemical ingredients and their pharmacological activities. Investigation of the chemical ingredients indicated that two new isoflavones, 2'-hydroxyerythrin A (1), and daidzein-7-O-β-d-{6″-[(E)-but-2-enoyl]}glycoside (2), together with seven known ones - 7,4'-dihydroxy-6-methoxyisoflavone (3), daidzein (4), daidzin (5), genistein (6), formononetin (7), ononin (8), and isoerythrinin A (9) - were obtained. The structures of compounds 1-9 were elucidated on the basis of spectroscopic and chemical analysis. We evaluated the antimicrobial efficacies and free-radical scavenging potential of the isolated compounds (1-9). Compounds 1 and 9 exhibited the most pronounced efficacy against the tested bacterial strains with IC 50 values ranging from 10.6 to 22.6 μg mL -1 . The isolated compounds showed moderate radical scavenging properties with compound 6 being the most active, followed by compounds 3, 1 and 4. This study indicated that the isoflavones from soya beans could be considered as potential antioxidants or antimicrobials in the food, cosmetics and pharmaceutical industries. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  8. Safety assessment of the biogenic amines in fermented soya beans and fermented bean curd.

    Science.gov (United States)

    Yang, Juan; Ding, Xiaowen; Qin, Yingrui; Zeng, Yitao

    2014-08-06

    To evaluate the safety of biogenic amines, high performance liquid chromatography (HPLC) was used to evaluate the levels of biogenic amines in fermented soya beans and fermented bean curd. In fermented soya beans, the total biogenic amines content was in a relatively safe range in many samples, although the concentration of histamine, tyramine, and β-phenethylamine was high enough in some samples to cause a possible safety threat, and 8 of the 30 samples were deemed unsafe. In fermented bean curd, the total biogenic amines content was more than 900 mg/kg in 19 white sufu amples, a level that has been determined to pose a safety hazard; putrescine was the only one detected in all samples and also had the highest concentration, which made samples a safety hazard; the content of tryptamine, β-phenethylamine, tyramine, and histamine had reached the level of threat to human health in some white and green sufu samples, and that may imply another potential safety risk; and 25 of the 33 samples were unsafe. In conclusion, the content of biogenic amines in all fermented soya bean products should be studied and appropriate limits determined to ensure the safety of eating these foods.

  9. [Determination of total protein content in soya-bean milk via visual moving reaction boundary titration].

    Science.gov (United States)

    Guo, Chengye; Wang, Houyu; Zhang, Lei; Fan, Liuyin; Cao, Chengxi

    2013-11-01

    A visual, rapid and accurate moving reaction boundary titration (MRBT) method was used for the determination of the total protein in soya-bean milk. During the process, moving reaction boundary (MRB) was formed by hydroxyl ions in the catholyte and soya-bean milk proteins immobilized in polyacrylamide gel (PAG), and an acid-base indicator was used to denote the boundary motion. The velocity of MRB has a relationship with protein concentration, which was used to obtain a standard curve. By paired t-test, there was no significant difference of the protein content between MRBT and Kjeldahl method at 95% confidence interval. The procedure of MRBT method required about 10 min, and it had linearity in the range of 2.0-14.0 g/L, low limit of detection (0.05 g/L), good precision (RSD of intra-day < 1.90% and inter-day < 4.39%), and high recoveries (97.41%-99.91%). In addition, non-protein nitrogen (NPN) such as melamine added into the soya-bean milk had weak influence on MRBT results.

  10. Influence of bitter lupin on consumption and digestibility in organic dairy cattle soya bean free diets

    Directory of Open Access Journals (Sweden)

    R. Tocci

    2010-04-01

    Full Text Available One of the main principles of organic husbandry is that animal feed must be GMO free, and soya bean is well-known as a high risk GMO alimentary source. About 25 dry dairy cattle of the Italian Holstein breed, from the Cooperativa Emilio Sereni of Borgo S. Lorenzo (FI, were fed in two successive diets: the first with extruded soya bean (A, and the second in which bitter lupin, faba bean and proteinic pea substituted the soya bean (B. We evaluated both the consumption and the apparent digestibility (using acid insoluble ash as internal marker of the two diets, repeating the trial twice. The presence of bitter lupin did not influence either the consumption of other feed, or the faecal water content. The apparent digestibility of the organic matter resulted satisfactory in both the diets, but was significantly higher in diet (A than in diet (B (71,6% vs 67,3%. In conclusion, even though we wish the cultivation of sweet lupin would be increase in Italy, we retain that also bitter lupin (mixed with other feed to increase the palatability could be used as alternative protein source in dairy cattle diets.

  11. Fermente bir Soya Ürünü “Miso”

    Directory of Open Access Journals (Sweden)

    Kamuran Ayhan

    2015-02-01

    Full Text Available Miso, Uzakdoğu’da üretilen ve insanlarca fazla miktarda tüketilen fermente bir soya ürünüdür. Miso üretiminde hammadde olarak soya fasulyesi, pirinç veya arpa, tuz ve mikroorganizma kültürü (Aspergillus oryzae kullanılmaktadır. Miso’nun rengi koyu kahverenginden açık sarıya kadar değişim göstermektedir. Fermentasyon sırasında soya fasulyesinde bulunan protein, karbonhidrat ve yağlar daha basit bileşim unsurlarına parçalanmaktadır. Miso, %12.5-19.0 protein, %1.4-6.9 yağ, %5.5-13 oranında tuz içermekte, lezzet verici bir gıda maddesi özelliği taşımaktadır. Miso özellikle esansiyel amino asitlerce zengindir. Protein kalitesini belirleyen NPU (Net protein kullanımı değeri misoda %72’dir. Protein kalitesi açısından bilinen protein kaynaklı gıdalar içinde ilk sırayı almaktadır.

  12. Características agronómicas de la soya en función de las densidades de siembra y profundidad de deposición de abono

    Directory of Open Access Journals (Sweden)

    Jorge Wilson Cortez

    2011-02-01

    Full Text Available El objetivo de este trabajo fue evaluar las características agronómicas de la soya sembrada en Sistema de Siembra Directa, en función de las densidades de siembra (15, 16 y 20 plantas por metro y la profundidad de deposición de abono (11, 14 y 17 cm. El experimento fue realizado en el Departamento de Ingeniería Rural, UNESP/Jaboticabal, utilizando, en el delineamiento, parcelas subdivididas en bloques al acaso, con cuatro repeticiones. Características agronómicas de la soya analizadas: número de días de aparición, índice de aparición, porcentaje de daños, altura de plantas, altura de inserción de la primera vaina, vainas por planta, granos por vaina, porcentaje de plantas sobrevivientes y productividad. Los resultados indicaron que el número de días de aparición, índice de aparición y el porcentaje de daños, no fueron influenciados por las densidades y la profundidad de deposición de abono. Los daños por insectos y aves fueron del orden de 30% de plantas atacadas. La altura de plantas, altura de inserción de la primera vaina, vainas por planta y granos por vaina no variaron en relación a la densidad y profundidad de deposición de abono. La productividad de la soya (5.146 kg ha-1 fue semejante en las densidades y profundidades de deposición de abono.

  13. CRISPR/Cas9-mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean.

    Science.gov (United States)

    Cai, Yupeng; Chen, Li; Liu, Xiujie; Guo, Chen; Sun, Shi; Wu, Cunxiang; Jiang, Bingjun; Han, Tianfu; Hou, Wensheng

    2018-01-01

    Flowering is an indication of the transition from vegetative growth to reproductive growth and has considerable effects on the life cycle of soya bean (Glycine max). In this study, we employed the CRISPR/Cas9 system to specifically induce targeted mutagenesis of GmFT2a, an integrator in the photoperiod flowering pathway in soya bean. The soya bean cultivar Jack was transformed with three sgRNA/Cas9 vectors targeting different sites of endogenous GmFT2a via Agrobacterium tumefaciens-mediated transformation. Site-directed mutations were observed at all targeted sites by DNA sequencing analysis. T1-generation soya bean plants homozygous for null alleles of GmFT2a frameshift mutated by a 1-bp insertion or short deletion exhibited late flowering under natural conditions (summer) in Beijing, China (N39°58', E116°20'). We also found that the targeted mutagenesis was stably heritable in the following T2 generation, and the homozygous GmFT2a mutants exhibited late flowering under both long-day and short-day conditions. We identified some 'transgene-clean' soya bean plants that were homozygous for null alleles of endogenous GmFT2a and without any transgenic element from the T1 and T2 generations. These 'transgene-clean' mutants of GmFT2a may provide materials for more in-depth research of GmFT2a functions and the molecular mechanism of photoperiod responses in soya bean. They will also contribute to soya bean breeding and regional introduction. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  14. THE EFFECT OF SOIL CONDITIONERS ON CELLULOSE, HEMICELLULOSE, AND THE ADL FIBRE FRACTION CONCENTRATION IN DACTYLIS GLOMERATA AND LOLIUM PERENNE

    Directory of Open Access Journals (Sweden)

    Milena Truba

    2017-01-01

    Full Text Available Replicated three times, the research was conducted in the experimental field between 2011 and 2014. Three soil conditioners with the following trade names: UGmax, Eko-Użyźniacz, and Humus Active Papka were used in the experiment, separately or together with NPK fertilisers. They were all used on plots sown with two species of grass, Dactylis glomerata of the Bora variety and Lolium perenne of the Info variety. The plant material from both grass species was tested for the concentration of ADL fraction (% DM, cellulose (% DM, and hemicellulose (% DM. It was found that the concentration of cellulose, hemicelluloses, and the ADL fraction was significantly higher in the biomass of Dactylis glomerata than in the biomass of Lolium perenne. The grass from the plot with the UGmax soil conditioner applied had the highest amount of cellulose and hemicellulose. The lowest amount of those organic compounds was found in the grass treated with UGmax together with mineral fertilisers and in plants treated with Humus Active, together with mineral fertilisers. However, the fertilisers and conditioners did not increase the ADL content in both grass species.

  15. STUDIES REGARDING THE CHELATE-INDUCED HYPERACCUMULATION OF CU AND FE USING LOLIUM PERENNE SPECIES IN MINING AREAS

    Directory of Open Access Journals (Sweden)

    ANCA-DIANA PRICOP

    2009-05-01

    Full Text Available The plant capacity to absorb high amounts of metal for a short period of time is the major factor that influences the efficiency of phytoextraction. The hyperaccumulating plants uptake high amounts in their tissues correlated to the metal concentrations in soil. Chelating agents have the capacity to induce the metal accumulation in biomass. They increase metal bioavailability for plants by releasing the metal in accessible forms. The present study emphasizes that in the case of EDTA use, the obtained biomass is smaller compared to the other variants, showing a lower tolerance to this chelating agent of Lolium perenne species. Cu and Fe phytoextraction by Lolium perenne species is higher in the case of EDTA use. Cu bioaccumulation has higher values in variants with compost-sterile mixture ratio of 1:4 in comparison with Fe. In the case of the best compost-sterile mixture ratio of 1:3 the highest biomass is obtained in all the variants, biosolids’ effect being stronger compared to the chelating agent.

  16. Efecto de algunos fungicidas sobre la interacción Rhizoctonia solani Kuhn-Micorriza vesículo arbuscular en soya, Glycine max Merril

    Directory of Open Access Journals (Sweden)

    Sánchez de Prager Marina

    1987-09-01

    Full Text Available En el campo se dispuso de dos preparaciones de suelo: natural y desinfectado químicamente (Ditrapex-CE y en el invernadero de suelo esterilizado con vapor. Se utilizaron los fungicidas Propamocarb, SN-84364, PCNB y Vitavax-300. Se contó con la flora micorrizógena natural y una cepa introducida, Glomus manihotis. R. solani disminuyó en un 50 % la emergencia de la soya, comportándose más agresivo en suelo desinfectado. En los primeros 15 días su ataque se incrementó y redujo el desarrollo de MVA en suelo natural. Al avanzar la edad de la planta decreció su infección. Con relación a la MVA la tendencia es contraria. La presencia de la MVA, incluyendo G. manihotis no incrementó significativamente la materia seca y el rendimiento de la soya. Al desinfectar el suelo los fungicidas afectaron negativamente la infección micorrizógena, mientras que en suelo natural no sucedió este fenómeno, al contrario SN-84364 incrementó su presencia. Este producto es el que menos afecta la simbiosis en el suelo desinfectado. Los fungicidas SN-84364 y PCNB mostraron gran especificidad contra R. solani y Vitavax-300 mayor espectro de acción.With the object to evaluate in the soybeans crop behavior in the interaction of Rhizoctonia solani, vesicular-arbuscular mycorrhizae (VAM and fungicides used to treatment of seed, two different soil preparations were used in the field trials: natural and chemically disinfected (Ditrapex-CE and under greenhouse: using vapor- sterilized. Was utilized the fungicides Propamocarb, SN-84364, PCNB y Vitavax- 300. Be had into account the natural mycorrhizal flora and a source of Glomus manihotis introduced. R. solani reduced the emergence of soybean by 50%, the above-metioned pathogen was more agressive in disinfected soil. In the 15 days first the attack increased and reduced the VAM development in the natural soil. With the age of the plant the pathogen infection decreased. With relation by VAM is contrary the tendency

  17. Effect of trypsin inhibitor activity in soya bean on growth performance, protein digestibility and incidence of sub-clinical necrotic enteritis in broiler chicken flocks.

    Science.gov (United States)

    Palliyeguru, M W C D; Rose, S P; Mackenzie, A M

    2011-06-01

    1. The effect of three different levels of dietary trypsin inhibitor activity (achieved by varying the amount of non-toasted full fat soya bean in replacement for toasted full fat soya bean) on the incidence of spontaneously-occurring sub-clinical necrotic enteritis (NE) in broiler chickens was compared. A fourth dietary treatment compared the effect of a diet that used potato protein concentrate as the major protein source. The determined trypsin inhibitor activity increased with the increasing content of non-toasted soya bean: 1·90, 6·21, 8·46 and 3·72 mg/g for the three soya bean diets (0, 100 and 200 g of non-toasted soya bean/kg) and the potato protein diet respectively. 2. Although increasing amounts of the non-toasted full-fat soya bean increased the feed intakes of the birds, there was a marked reduction in protein digestibility, weight gain and feed conversion efficiency. 3. There was a linear increase in sub-clinical NE lesions in the duodenum, jejunum, mid small intestine and ileum with increasing non-toasted soya bean. Caecal Clostridium perfringens counts increased with the increasing dietary content of non-toasted soya bean. Serum α-toxin antibodies were higher in the birds fed the 200 g non-toasted soya bean/kg diet compared with the other diets. 4. The results demonstrated that variation in the amount of non-toasted dietary soya bean not only affects growth performance of broilers but also affects the incidence of sub-clinical necrotic enteritis in the flock. Ensuring the lowest possible trypsin-inhibitor activity in soya bean samples is a valuable tool to improve the health and welfare of birds and in reducing the financial losses from this disease.

  18. Intake patterns and dietary associations of soya protein consumption in adults and children in the Canadian Community Health Survey, Cycle 2.2.

    Science.gov (United States)

    Mudryj, Adriana N; Aukema, Harold M; Yu, Nancy

    2015-01-28

    Soya foods are one of the recommended alternatives to meat in many dietary guidelines. While this is expected to increase the intake of some nutrients, potential concerns regarding others have been raised. The purpose of the present study was to examine the prevalence and the association of soya food consumption with nutrient intakes and dietary patterns of Canadians (age ≥ 2 years). Cross-sectional data from the 2004 Canadian Community Health Survey (Cycle 2.2; n 33,218) were used to classify soya consumers and non-consumers. Soya consumers were further divided into two groups based on their soya protein intake. Sample weights were applied and logistic regression analysis was used to explore the association between nutrient intakes and soya consumption, with cultural background, sex, age and economic status being included as covariates. On any given day, 3.3% (n 1085) of Canadians consume soya foods, with females, Asian Canadians and adults with post-secondary education being more likely to be soya consumers. As a whole, adolescent and adult respondents who had consumed at least one soya food during their 24 h dietary recall had higher energy intakes, as well as increased intakes of nutrients such as protein, fibre, vitamin C, vitamin B6, naturally occurring folate, thiamin, Ca, P, Mg, PUFA, Fe and K and lowered intakes of saturated fat. These data indicate that soya food consumption is associated with improved diet quality of Canadians. However, future research is necessary to investigate the association between increased energy intake and soya consumption.

  19. Ergot alkaloid intoxication in perennial ryegrass (Lolium perenne): an emerging animal health concern in Ireland?

    Science.gov (United States)

    Canty, Mary J; Fogarty, Ursula; Sheridan, Michael K; Ensley, Steve M; Schrunk, Dwayne E; More, Simon J

    2014-01-01

    Four primary mycotoxicosis have been reported in livestock caused by fungal infections of grasses or cereals by members of the Clavicipitaceae family. Ergotism (generally associated with grasses, rye, triticale and other grains) and fescue toxicosis (associated with tall fescue grass, Festuca arundinacea) are both caused by ergot alkaloids, and referred to as 'ergot alkaloid intoxication'. Ryegrass staggers (associated with perennial ryegrass Lolium perenne) is due to intoxication with an indole-diperpene, Lolitrem B, and metabolites. Fescue-associated oedema, recently described in Australia, may be associated with a pyrrolizidine alkaloid, N-acetyl norloline. Ergotism, caused by the fungus Claviceps purpurea, is visible and infects the outside of the plant seed. Fescue toxicosis and ryegrass staggers are caused by Neotyphodium coenophalium and N. lolii, respectively. Fescue-associated oedema has been associated with tall fescue varieties infected with a specific strain of N. coenophialum (AR542, Max P or Max Q). The name Neotyphodium refers to asexual derivatives of Epichloë spp., which have collectively been termed the epichloë fungi. These fungi exist symbiotically within the grass and are invisible to the naked eye. The primary toxicological effect of ergot alkaloid involves vasoconstriction and/or hypoprolactinaemia. Ingestion of ergot alkaloid by livestock can cause a range of effects, including poor weight gain, reduced fertility, hyperthermia, convulsions, gangrene of the extremities, and death. To date there are no published reports, either internationally or nationally, reporting ergot alkaloid intoxication specifically associated with perennial ryegrass endophytes. However, unpublished reports from the Irish Equine Centre have identified a potential emerging problem of ergot alkaloid intoxication with respect to equines and bovines, on primarily perennial ryegrass-based diets. Ergovaline has been isolated in varying concentrations in the herbage of a

  20. Comparison of the fatty acid composition of fresh and ensiled perennial ryegrass (Lolium perenne L.), affected by cultivar and regrowth interval

    NARCIS (Netherlands)

    Elgersma, A.; Ellen, G.; Horst, van der H.; Muuse, B.G.; Boer, H.; Tamminga, S.

    2003-01-01

    Two experiments were conducted to evaluate the effects of grass cultivar and regrowth stage on the fatty acid (FA) profile in fresh and ensiled perennial ryegrass (Lolium perenne L.). The Experiment 1 compared the composition of fresh grass with that of pre-wilted ensiled material of six cultivars,

  1. Coordinated nitrogen and carbon remobilization for nitrate assimilation in leaf, sheath and root and associated cytokinin signals during early regrowth of Lolium perenne

    Czech Academy of Sciences Publication Activity Database

    Roche, J.; Turnbull, M. H.; Guo, Q.; Novák, Ondřej; Späth, J.; Gieseg, S. P.; Jameson, P. E.; Love, J.

    2017-01-01

    Roč. 119, č. 8 (2017), s. 1353-1364 ISSN 0305-7364 R&D Projects: GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : assimilation * cytokinin * defoliation * Lolium perenne * nitrate * Nitrogen * regrowth * water-soluble carbohydrates Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Plant sciences, botany Impact factor: 4.041, year: 2016

  2. A statistical mixture model for estimating the proportion of unreduced pollen grains in perennial ryegrass (Lolium perenne L.) via the size of pollen grains

    NARCIS (Netherlands)

    Jansen, R.C.; Nijs, A.P.M. den

    1993-01-01

    The size of pollen grains is commonly used to indicate the ploidy level of pollen grains. In this paper observations of the diameter of pollen grains are evaluated from one diploid accession of perennial ryegrass (Lolium perenne L.), which was expected to produce diploid (unreduced) pollen grains in

  3. Soya-cerebroside, an extract of Cordyceps militaris, suppresses monocyte migration and prevents cartilage degradation in inflammatory animal models

    Science.gov (United States)

    Liu, Shan-Chi; Chiu, Ching-Peng; Tsai, Chun-Hao; Hung, Chun-Yin; Li, Te-Mao; Wu, Yang-Chang; Tang, Chih-Hsin

    2017-01-01

    Pathophysiological events that modulate the progression of structural changes in osteoarthritis (OA) include the secretion of inflammatory molecules, such as proinflammatory cytokines. Interleukin-1beta (IL-1β) is the prototypical inflammatory cytokine that activates OA synovial cells to release cytokines and chemokines in support of the inflammatory response. The monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes in response to inflammation. We show in this study that IL-1β-induced MCP-1 expression and monocyte migration in OA synovial fibroblasts (OASFs) is effectively inhibited by soya-cerebroside, an extract of Cordyceps militaris. We found that soya-cerebroside up-regulated of microRNA (miR)-432 expression via inhibiting AMPK and AKT signaling pathways in OASFs. Soya-cerebroside also effectively decreased monocyte infiltration and prevented cartilage degradation in a rat inflammatory model. Our findings are the first to demonstrate that soya-cerebroside inhibits monocyte/macrophage infiltration into synoviocytes, attenuating synovial inflammation and preventing cartilage damage by reducing MCP-1 expression in vitro and in vivo. Taken together, we suggest a novel therapeutic strategy based on the use of soya-cerebroside for the management of OA. PMID:28225075

  4. Dietary soya saponins increase gut permeability and play a key role in the onset of soyabean-induced enteritis in Atlantic salmon (Salmo salar L.)

    DEFF Research Database (Denmark)

    Knudsen, Sven David Lausten; Jutfelt, Fredrik; Sundh, Henrik

    2008-01-01

    are naturally occurring amphiphilic molecules and have been associated with many biological activities. The aim of the present study was to investigate whether soya saponins trigger the onset of soyabean-induced enteritis in Atlantic salmon (Salmo salar L.), and to examine if dietary soya saponins...... increase the epithelial permeability of the distal intestine in Atlantic salmon. Seven experimental diets containing different levels of soya saponins were fed to seawater-adapted Atlantic salmon for 53 d. The diets included a fishmeal-based control diet, two fishmeal-based diets with different levels...... of added soya saponins, one diet containing 25 % lupin kernel meal, two diets based on 25 % lupin kernel meal with different levels of added soya saponins, and one diet containing 25 % defatted soyabean meal. The effect on intestinal morphology, intestinal epithelial permeability and faecal DM content...

  5. Dietary soya saponins increase gut permeability and play a key role in the onset of soyabean-induced enteritis in Atlantic salmon ( Salmo salar L.).

    Science.gov (United States)

    Knudsen, David; Jutfelt, Fredrik; Sundh, Henrik; Sundell, Kristina; Koppe, Wolfgang; Frøkiaer, Hanne

    2008-07-01

    Saponins are naturally occurring amphiphilic molecules and have been associated with many biological activities. The aim of the present study was to investigate whether soya saponins trigger the onset of soyabean-induced enteritis in Atlantic salmon (Salmo salar L.), and to examine if dietary soya saponins increase the epithelial permeability of the distal intestine in Atlantic salmon. Seven experimental diets containing different levels of soya saponins were fed to seawater-adapted Atlantic salmon for 53 d. The diets included a fishmeal-based control diet, two fishmeal-based diets with different levels of added soya saponins, one diet containing 25% lupin kernel meal, two diets based on 25% lupin kernel meal with different levels of added soya saponins, and one diet containing 25% defatted soyabean meal. The effect on intestinal morphology, intestinal epithelial permeability and faecal DM content was examined. Fish fed 25% defatted soyabean meal displayed severe enteritis, whereas fish fed 25% lupin kernel meal had normal intestinal morphology. The combination of soya saponins and fishmeal did not induce morphological changes but fish fed soya saponins in combination with lupin kernel meal displayed significant enteritis. Increased epithelial permeability was observed in fish fed 25% defatted soyabean meal and in fish fed soya saponin concentrate independent of the protein source in the feed. The study demonstrates that soya saponins, in combination with one or several unidentified components present in legumes, induce an inflammatory reaction in the distal intestine of Atlantic salmon. Soya saponins increase the intestinal epithelial permeability but do not, per se, induce enteritis.

  6. Soya bean meal increases litter moisture and foot pad dermatitis in maize and wheat based diets for turkeys but maize and non-soya diets lower body weight.

    Science.gov (United States)

    Hocking, P M; Vinco, L J; Veldkamp, T

    2018-04-01

    1. A 2 × 2 factorial experiment was conducted to compare the effects of wheat or maize based diets differing in dietary electrolyte balance (DEB) on litter moisture and foot pad dermatitis (FPD) at 4, 8 and 12 weeks of age in heavy-medium turkeys. A second objective was to investigate the effects on foot pad dermatitis of the interaction between dietary composition and artificially increasing litter moisture by adding water to the litter. 2. High DEB diets contained soya as the main protein source whereas low DEB diets did not contain soya bean meal. Diets were formulated to be iso-caloric and iso-nitrogenous in each of 3 successive 4-week phases following recommended dietary compositions. DEB concentrations were 330, 290 and 250 mEq/kg in high DEB diets and 230, 200 and 180 mEq/kg in low DEB diets. 3. Litter moisture and mean FPD score were higher in turkeys fed on high DEB diets compared with low DEB diets whereas there was no difference between maize and wheat. 4. Food intake was similar and body weight was lower after litter moisture was artificially raised in the wet compared with the dry litter treatment and there was no interaction with dietary composition. 5. Mean body weight and feed intake were higher in turkeys fed on wheat compared with maize and in high DEB compared with low DEB diets at 12 weeks of age. 6. Lowering dietary DEB for turkeys may improve litter moisture and lower the prevalence of FPD in commercial turkey flocks.

  7. Soya oil-based shampoo superior to 0.5% permethrin lotion for head louse infestation

    Directory of Open Access Journals (Sweden)

    Nazma A Burgess

    2011-03-01

    Full Text Available Ian F Burgess1, Katrina Kay1,2, Nazma A Burgess1, Elizabeth R Brunton11Medical Entomology Centre, Insect Research and Development Limited, Cambridge, 2Leeds Primary Care Trust, Leeds, UKBackground: This was a randomized, assessor-blind, controlled comparison of a soya oil-based medical device shampoo with a medicinal permethrin lotion in an alcohol vehicle for treatment of head louse infestation to generate data suitable for a regulatory submission to achieve reimbursable status for the shampoo product.Methods: We treated 91 children and adults, divided between two sites, on two occasions 9 days apart. Participants washed their hair and towel-dried it before treatment. The shampoo was used twice for 30 minutes each time. The lotion was used for 30 minutes followed by rinsing. Assessments were made by dry detection combing on days 2, 9, 11, and 14 after the first treatment. According to present knowledge, this combing technique does not influence the overall head louse populations or outcome of treatment.Results: The soya oil shampoo was significantly (P < 0.01 more effective than the lotion for both intention to treat (62.2% versus 34.8% successful treatment and per-protocol (74.3% versus 36.8% success groups. Post-treatment assessments showed the necessity for repeat treatment, but that a 9-day interval was too long because if eggs hatched after the first treatment, the lice could grow old enough to lay eggs before the second treatment.Conclusion: The soya oil-based shampoo was more effective than the permethrin lotion, more cosmetically acceptable, and less irritant.Keywords: pediculosis, medical device, medicinal product, insecticide, vegetable oil

  8. Soya oil-based shampoo superior to 0.5% permethrin lotion for head louse infestation [Corrigendum

    Directory of Open Access Journals (Sweden)

    Burgess IF

    2015-07-01

    Full Text Available Burgess IF, Kay K, Burgess NA, Brunton ER. Med Devices (Auckl. 2011;4:35-42 In March 2011 we published a report of a clinical study investigating the efficacy of a shampoo for the treatment of head louse infestation.1 The report states that the shampoo used contained, as an active component, 1% soya oil. However, it has now come to light that this product may have a considerably different formulation, and possibly contained no soya oil at all. Read the original article

  9. Effect of oil refining processes on 14 C-tetrachlorvinphos residues in soya bean oil

    International Nuclear Information System (INIS)

    Farghaly, M.; Zayed, S.M.A.D.

    1986-01-01

    Crude soya bean oil extracted from grains treated with 14 C-tetrachlorvinphos and stored for 30 weeks was subjected to different refining processes. The effect of commercial refining processes, namely: alkali treatment, bleaching, winterization and deodorization on the nature and magnitude of the originally present residues was investigated. A high percentage (52%) of the residues was eliminated during alkali neutralization. No potentially toxic materials were detected among the identified degradation products. The obtained data showed that the ultimate degradation products were dimethyl phosphate and mono methyl phosphate.2 tab.,2 scheme

  10. Participation of Taxifolin in the Protection of Soya Seeds from the Effects of Heavy Metal Salts

    Directory of Open Access Journals (Sweden)

    V.A. Kuznetsova

    2015-06-01

    Full Text Available A correlation was revealed between the specific activity of peroxidases and their multiple forms during the germination of soya seeds (Glycine max (L. Merrill in the presence of heavy metal salts. It was shown that lead and cadmium sulfates cause emergence of new forms of the enzyme with high electrophoretic mobility, which indicates that the identified enzyme forms are involved in the molecular mechanism of adaptation to oxidative stress. Addition of taxifolin (dihydroquercetin, a bioflavonoid antioxidant, to the salts of heavy metals caused decrease in the specific activity of peroxidases and favored emergence of new forms of the enzyme, which were absent in the control samples.

  11. Evaluation of a cost effective technique for treating aquaculture water discharge using Lolium perenne Lam as a biofilter.

    Science.gov (United States)

    Nduwimana, André; Yang, Xiang-Long; Wang, Li-Ren

    2007-01-01

    Wastewater stabilization ponds generate low cost by-products that are useful for agriculture. The utilization of these by-products for soil amendment and as a source of nutrients for plants requires a high level of sanitation and stabilization of the organic matter, to maintain acceptable levels of soil, water and air quality. In this study, two aquaculture wastewater treatment systems; recirculating system and a floating plant bed system were designed to improve the quality of irrigation water in local communities with low income. In both systems the grass species Lolium perenne Lam was used as a plant biofilter while vegetable specie Amaranthus viridis was used to evaluate the performance of the system and the suitability of the phyto-treated water for irrigation. It was found that the harmful material removal rate for recirculating system was 88.9% for TAN (total ammonia nitrogen), 90% for NO2(-)-N, 64.8% for NO3(-)-N while for floating plant bed system 82.7% for TAN, 82% for NO2(-)-N and 60.5% for NO3(-)-N. Comparative analysis of the efficiency of waste element removal between the two systems revealed that both systems performed well, however, plant growth was not robust for floating plant bed system while recirculating system is energy consuming. Although both systems did not attain sufficient levels of TN (total nitrogen) and TP (total phosphorus) load reduction, the treatment with L. perenne remarkably improved the irrigation water quality. A. viridis plants irrigated with the phyto-treated discharge water had lesser concentrations of heavy metals in their tissues compared to those irrigated with untreated discharge. The control plants irrigated with untreated discharge were also found to be highly lignified with few stems and small leaves.

  12. Total tract nutrient digestion and milk fatty acid profile of dairy cows fed diets containing different levels of whole raw soya beans.

    Science.gov (United States)

    Venturelli, B C; de Freitas Júnior, J E; Takiya, C S; de Araújo, A P C; Santos, M C B; Calomeni, G D; Gardinal, R; Vendramini, T H A; Rennó, F P

    2015-12-01

    Whole oilseeds such as soya beans have been utilized in dairy rations to supply additional fat and protein. However, antinutritional components contained in soya beans, such as trypsin inhibitors and haemagglutinins (lectins) may alter digestibility of nutrients and consequently affect animal performance. The objective of the present experiment was to quantify the effect of different levels of whole raw soya beans in diets of dairy cows on nutrient intake, total tract digestion, nutrient balances and milk yield and composition. Sixteen mid to late-lactation cows (228 ± 20 days in milk; mean ± SD) were used in four replicated 4 × 4 Latin square design experiment with 21-d periods. Cows were assigned to each square according to milk yield and DIM. The animals were randomly allocated to treatments: control (without soya beans addition; CO), WS9, WS18 and WS27, with addition of 9%, 18% and 27% of whole raw soya bean in diet on a dry matter (DM) basis respectively. All diets contained identical forage and concentrate components and consisted of maize silage and concentrate based on ground corn and soya beans at a ratio of 60:40. There were no differences in OM, CP, NDF and NEL intakes (kg/day and MJ/day) among the treatments (p > 0.05). However, DM and NFC intakes were negatively affected (p = 0.04 and p raw soya beans for EE (p raw soya beans. However, the nutritive characteristics of excreted grains were not altered. Milk (kg), milk lactose (kg) and protein (kg) yield decreased linearly (p milk fat content (%) increased linearly (p raw soya beans inclusion. Increasing addition of whole raw soya beans affected milk fatty acid profile with a linear decrease of cis-9-trans 11CLA and total saturated FA; and linear increase of total unsaturated and C18:3 FA. Energy balance was positively affected (p = 0.03) by whole raw soya beans as well as efficiency of NEL milk/DE intake (p = 0.02). Nitrogen balance and microbial protein synthesis were not affected by whole raw soya

  13. Comparative Studies on Dyeability with Direct, Acid and Reactive Dyes after Chemical Modification of Jute with Mixed Amino Acids Obtained from Extract of Waste Soya Bean Seeds

    Science.gov (United States)

    Bhaumik, Nilendu Sekhar; Konar, Adwaita; Roy, Alok Nath; Samanta, Ashis Kumar

    2017-12-01

    Jute fabric was treated with mixed natural amino acids obtained from waste soya bean seed extract for chemical modification of jute for its cataionization and to enhance its dyeability with anionic dyes (like direct, reactive and acid dye) as well enabling soya modified jute for salt free dyeing with anionic reactive dyes maintaining its eco-friendliness. Colour interaction parameters including surface colour strength were assessed and compared for both bleached and soya-modified jute fabric for reactive dyeing and compared with direct and acid dye. Improvement in K/S value (surface colour strength) was observed for soya-modified jute even in absence of salt applied in dye bath for reactive dyes as well as for direct and acid dyes. In addition, reactive dye also shows good dyeability even in acid bath in salt free conditions. Colour fastness to wash was evaluated for bleached and soya-modified jute fabric after dyeing with direct, acid and reactive dyes are reported. Treatment of jute with soya-extracted mixed natural amino acids showed anchoring of some amino/aldemine groups on jute cellulosic polymer evidenced from Fourier Transform Infra-Red (FTIR) Spectroscopy. This amino or aldemine group incorporation in bleached jute causes its cationization and hence when dyed in acid bath for reactive dye (instead of conventional alkali bath) showed dye uptake for reactive dyes. Study of surface morphology by Scanning Electron Microscopy (SEM) of said soya-modified jute as compared to bleached jute was studied and reported.

  14. Multifamily determination of pesticide residues in soya-based nutraceutical products by GC/MS-MS.

    Science.gov (United States)

    Páleníková, Agneša; Martínez-Domínguez, Gerardo; Arrebola, Francisco Javier; Romero-González, Roberto; Hrouzková, Svetlana; Frenich, Antonia Garrido

    2015-04-15

    An analytical method based on a modified QuEChERS extraction coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS) was evaluated for the determination of 177 pesticides in soya-based nutraceutical products. The QuEChERS method was optimised and different extraction solvents and clean-up approaches were tested, obtaining the most efficient conditions with a mixture of sorbents (PSA, C18, GBC and Zr-Sep(+)). Recoveries were evaluated at 10, 50 and 100 μg/kg and ranged between 70% and 120%. Precision was expressed as relative standard deviation (RSD), and it was evaluated for more than 160 pesticides as intra and inter-day precision, with values always below 20% and 25%, respectively. Limits of detection (LODs) ranged from 0.1 to 10 μg/kg, whereas limits of quantification (LOQs) from 0.5 to 20 μg/kg. The applicability of the method was proved by analysing soya-based nutraceuticals. Two pesticides were found in these samples, malathion and pyriproxyfen, at 11.1 and 1.5 μg/kg respectively. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. Radioprotective effects of soya and garlic oils in irradiated male rats

    International Nuclear Information System (INIS)

    Shabon, M.H.

    2005-01-01

    Forty male albino rats were divided equally into four groups, the first group acted as control group, the second group was subjected to 5 Gy whole body gamma irradiation, the third group was injected intraperitoneally daily with soya oil (15 mg/kg b.w. for 14 days) before gamma irradiation and the fourth group was injected intraperitoneally daily with garlic oil (150/kg b.w. for 14 days) before gamma irradiation. Mortality percentage and body weight were recorded. Blood samples were collected one week post-gamma irradiation from all animals for biochemical analysis. Serum levels of thiobarbituric acid reactive substance (TBARS), total lipids, triglycerides and cholesterol were measured. Total proteins and albumin were measured using photometric methods. Globulin value was calculated by subtraction of albumin from total protein values. The results showed that gamma irradiation with dose level of 5 Gy induced retardation of live body weight and increased the mortality. The data also showed significant increases in TBARS, total lipids, triglycerides and cholesterol levels, whereas the levels of total proteins, albumin and globulin were significantly decreased. The soya oil or garlic oil injected pre-irradiation exerted noticeable amelioration in induced radiation changes on body weight, mortality, TBARS, lipid profiles and protein fractions

  16. Degradation of 14C-Lindane(gamma-hexachlorocyclohexane) in Corn and Soya Bean Seedlings

    International Nuclear Information System (INIS)

    Afifi, M.L.

    2003-01-01

    The degradation of lindane (gamma-hexachlorocyclohexane,(gamma-HCH) by corn and soya bean seedlings has been studied. Following root application, anumber of metabolic products were identified utilizing Gas-liquid chromatography and GLC-mass spectrometry. In corn seedlings, these metabolites proved to be m-dichlorobenzene;1,2,4-trichlorobenzene; 1,2,4,5-tetrachlorobenzene; 2,4,5-and 2,3,5-trichlorophenols and gamma-pentachlorocyclohex-1-ene as compared with the standards. In soya bean seedlings, however, while 1,2,3,4-tetrachlorobenzene and 2,3,5-trichlorophenol were missing, 1,2,3-trichlorobenzene and 2,4,6-trichlorophenol were produced. Of the total chlorinated compounds found in hexane extracts, these metabolic products accounted for 30-35%. The amount of trichlorophenols which contributed to 38% and 16% of the metabolic output in corn and peas respectively, provided futher evidence that the degrading pathways in monocots might differ from these in dicots. The results also show that in both plant tissues dechlorination generally took place and represented adominant intermediary detoxification process

  17. Effect of Preservative on the Shelf Life of Yoghurt Produced from Soya Beans Milk

    Directory of Open Access Journals (Sweden)

    Uduak G. AKPAN

    2007-09-01

    Full Text Available This study concentrated on the effects of preservatives on shelf life of yoghurt produced from Soya beans milk. The yoghurt was produced by heating Soya beans milk slurry, cooled and incubated with starter culture. After the required yoghurt has been formed, sugar, flavour and preservatives were added. Study of the effect of preservatives revealed that Sodium benzoate preservative used at 20mg/ml give the best (optimum preservation on both shelf and refrigeration storage for 15 and 21 days respectively. This is because the inhibitive ability of Sodium benzoate at lower temperature is higher than that of Potassium metabisulphate preservative. The study also revealed that 40mg/ml concentration of the combined preservatives gives the best (optimum concentration level for both shelf and refrigeration storage with pH values of 3.92 and 4.01 respectively after 14 days fermentation. The preservatives concentration added are within the threshold values specified by Standard Organization of Nigeria (SON and National Agency for Food Administration and Control (NAFDAC.

  18. Asociación entre el crecimiento bacteriano de la flora intestinal y la presión arterial de hombres adultos mayores hipertensos después de cinco semanas de consumo de soya (Glicine máx

    Directory of Open Access Journals (Sweden)

    Celia Rossi Trespalacios

    2014-01-01

    Full Text Available Objetivo: Objetivo: Determinar la asociación entre el crecimiento bacteriano de la flora intestinal y la presión arterial de hombres adultos mayores hipertensos después de cinco semanas de consumo de soya (Glicine máx. Materiales y métodos: Estudio cuasiexperimental para evaluar la asociación entre cambios en la presión arterial y el crecimiento bacteriano en 24 hombres hipertensos con edades entre 61 y 82 años residentes en el barrio Las Malvinas (suroccidente de Barranquilla, quienes adicionaron a su dieta habitual “leche de soya” de grano entero sin lactosa (18 gramos de proteína de soya por día durante 6 semanas. En el análisis microbiológico de las heces se determinaron los géneros y la cantidad de bacterias mediante técnicas tradicionales. Se midió la presión arterial al inicio y final del tratamiento. Resultados: Los recuentos de bacterias aeróbicas al inicio fueron 10 5 a 10 9 y al final 10 6 a 10 9 UFC/gramo de muestra; en bacterias anaeróbicas se encontraron los recuentos más altos: 10 5 a 10 9 antes y 10 6 a 1010 UFC/g al final. Después del tratamiento con soya la presión sistólica disminuyó en 37.5 % de los participantes, la diastólica en 16.67 %, la media en 45.83 % y la de pulso en 45.83 %; sin embargo, no hubo significancia estadística en ningún caso. El coeficiente de correlación de Pearson entre presión arterial y crecimiento bacteriano fue 0.14. Conclusiones: Se observó una asociación débil e inversa entre el incremento de la microbiota intestinal y la disminución de la presión arterial.

  19. Soya milk exerts different effects on plasma amino acid responses and incretin hormone secretion compared with cows' milk in healthy, young men.

    Science.gov (United States)

    Sun, Lijuan; Tan, Kevin Wei Jie; Siow, Phei Ching; Henry, Christiani Jeyakumar

    2016-10-01

    Apart from the well-known action of insulin, the mechanism by which soya and cows' milk improve postprandial glycaemia control was examined. In total, twelve healthy, young, Chinese men were studied on three separate occasions, in random order with isovolumetric (322 ml) control water, soya milk and cows' milk. Plasma total amino acid concentrations increased 30 min after test meals consumption and were higher after soya milk (230 %) and cow milk (240 %) consumption compared with water. Cows' milk ingestion induced higher branched-chain amino acids (BCAA) (40 %) than soya milk. Postprandial incretin concentrations increased after meal consumption. Cows' milk meal was accompanied by higher incremental AUC (iAUC) (170 %) for glucagon-like peptide-1 (GLP-1) compared with soya milk and control (P=0·06). However, glucose-dependent insulinotropic polypeptide (GIP) concentrations increased to significantly greater levels after soya milk consumption (iAUC 60 % higher) compared with cows' milk and control. Consumption of both soya and cows' milk with carbohydrates induced a similar reduction in glycaemic response through a different mechanism, beyond insulin action. Plasma amino acids (alanine and arginine), and incretins in particular (GIP was stimulated), may be involved in the hyperinsulinaemia after soya milk meals. However, BCAA and GLP-1 release may be responsible for the reduced glycaemia after cows' milk consumption by delaying gastric emptying. This could be the result of different milk protein/amino acid composition, but also differences in milk carbohydrate composition (i.e. lactose v. sucrose). It can be concluded that soya milk is a good alternative to cows' milk with regard to glycaemic regulation, with different mechanisms involved.

  20. Identification of prenylated pterocarpans and other isoflavonoids in Rhizopus spp. elicited soya bean seedlings by electrospray ionisation mass spectrometry.

    Science.gov (United States)

    Simons, Rudy; Vincken, Jean-Paul; Bohin, Maxime C; Kuijpers, Tomas F M; Verbruggen, Marian A; Gruppen, Harry

    2011-01-15

    Phytoalexins from soya are mainly characterised as prenylated pterocarpans, the glyceollins. Extracts of non-soaked and soaked soya beans, as well as that of soya seedlings, grown in the presence of Rhizopus microsporus var. oryzae, were screened for the presence of prenylated flavonoids with a liquid chromatography/mass spectrometry (LC/MS)-based screening method. The glyceollins I-III and glyceollidins I-II, belonging to the isoflavonoid subclass of the pterocarpans, were tentatively assigned. The formation of these prenylated pterocarpans was accompanied by that of other prenylated isoflavonoids of the subclasses of the isoflavones and the coumestans. It was estimated that approx. 40% of the total isoflavonoid content in Rhizopus-challenged soya bean seedlings were prenylated pterocarpans, whereas 7% comprised prenylated isoflavones and prenylated coumestans. The site of prenylation (A-ring or B-ring) of the prenylated isoflavones was tentatively annotated using positive-ion mode MS by comparing the (1,3) A(+) retro-Diels-Alder (RDA) fragments of prenylated and non-prenylated isoflavones. Furthermore, the fragmentation pathways of the five pterocarpans in negative-ion (NI) mode were proposed, which involved the cleavage of the C-ring and/or D-ring. The absence of the ring-closed prenyl (pyran or furan) gave exclusively -H(2) O(x,y) RDA fragments, whereas its presence gave predominantly the common RDA fragments. Copyright © 2010 John Wiley & Sons, Ltd.

  1. Influence of cultivar specificity on Cs 137 and Sr 90 accumulation in green part and seeds of soya plants

    International Nuclear Information System (INIS)

    Gutseva, G.Z.; Goloveshkin, V.V.

    2008-01-01

    As a result of investigation carried out with soya sorts zoned in Belarus on soddy-podsolic sandy soils contaminated as a result of Chernobyl accident with Cs 137 and Sr 90 a high sort selectivity in accumulation of the radionuclides is established. (authors)

  2. The application of Near-Infrared Reflectance Spectroscopy (NIRS) to detect melamine adulteration of soya bean meal.

    Science.gov (United States)

    Haughey, Simon A; Graham, Stewart F; Cancouët, Emmanuelle; Elliott, Christopher T

    2013-02-15

    Soya bean products are used widely in the animal feed industry as a protein based feed ingredient and have been found to be adulterated with melamine. This was highlighted in the Chinese scandal of 2008. Dehulled soya (GM and non-GM), soya hulls and toasted soya were contaminated with melamine and spectra were generated using Near Infrared Reflectance Spectroscopy (NIRS). By applying chemometrics to the spectral data, excellent calibration models and prediction statistics were obtained. The coefficients of determination (R(2)) were found to be 0.89-0.99 depending on the mathematical algorithm used, the data pre-processing applied and the sample type used. The corresponding values for the root mean square error of calibration and prediction were found to be 0.081-0.276% and 0.134-0.368%, respectively, again depending on the chemometric treatment applied to the data and sample type. In addition, adopting a qualitative approach with the spectral data and applying PCA, it was possible to discriminate between the four samples types and also, by generation of Cooman's plots, possible to distinguish between adulterated and non-adulterated samples. Copyright © 2012 Elsevier Ltd. All rights reserved.

  3. Transgenic soya bean seeds accumulating β-carotene exhibit the collateral enhancements of oleate and protein content traits.

    Science.gov (United States)

    Schmidt, Monica A; Parrott, Wayne A; Hildebrand, David F; Berg, R Howard; Cooksey, Amanda; Pendarvis, Ken; He, Yonghua; McCarthy, Fiona; Herman, Eliot M

    2015-05-01

    Transgenic soya bean (Glycine max) plants overexpressing a seed-specific bacterial phytoene synthase gene from Pantoea ananatis modified to target to plastids accumulated 845 μg β carotene g(-1) dry seed weight with a desirable 12:1 ratio of β to α. The β carotene accumulating seeds exhibited a shift in oil composition increasing oleic acid with a concomitant decrease in linoleic acid and an increase in seed protein content by at least 4% (w/w). Elevated β-carotene accumulating soya bean cotyledons contain 40% the amount of abscisic acid compared to nontransgenic cotyledons. Proteomic and nontargeted metabolomic analysis of the mid-maturation β-carotene cotyledons compared to the nontransgenic did not reveal any significant differences that would account for the altered phenotypes of both elevated oleate and protein content. Transcriptomic analysis, confirmed by RT-PCR, revealed a number of significant differences in ABA-responsive transcripton factor gene expression in the crtB transgenics compared to nontransgenic cotyledons of the same maturation stage. The altered seed composition traits seem to be attributed to altered ABA hormone levels varying transcription factor expression. The elevated β-carotene, oleic acid and protein traits in the β-carotene soya beans confer a substantial additive nutritional quality to soya beans. © 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  4. Evaluation of indigenous bacterial strains for biocontrol of the frogeye leaf spot of soya bean caused by Cercospora sojina.

    Science.gov (United States)

    Simonetti, E; Carmona, M A; Scandiani, M M; García, A F; Luque, A G; Correa, O S; Balestrasse, K B

    2012-08-01

    Assessment of biological control of Cercospora sojina, causal agent of frogeye leaf spot (FLS) of soya bean, using three indigenous bacterial strains, BNM297 (Pseudomonas fluorescens), BNM340 and BNM122 (Bacillus amyloliquefaciens). From cultures of each bacterial strain, cell suspensions and cell-free supernatants were obtained and assayed to determine their antifungal activity against C. sojina. Both mycelial growth and spore germination in vitro were more strongly inhibited by bacterial cell suspensions than by cell-free supernatants. The Bacillus strains BNM122 and BNM340 inhibited the fungal growth to a similar degree (I ≈ 52-53%), while cells from P. fluorescens BNM297 caused a lesser reduction (I ≈ 32-34%) in the fungus colony diameter. The foliar application of the two Bacillus strains on soya bean seedlings, under greenhouse conditions, significantly reduced the disease severity with respect to control soya bean seedlings and those sprayed with BNM297. This last bacterial strain was not effective in controlling FLS in vivo. Our data demonstrate that the application of antagonistic bacteria may be a promising and environmentally friendly alternative to control the FLS of soya bean.   To our knowledge, this is the first report of biological control of C. sojina by using native Bacillus strains. © 2012 The Authors. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.

  5. 40 CFR 721.10056 - Benzenemethanaminium, N-(3-aminopropyl)-N,N-dimethyl-, N-soya acyl derivs., chlorides.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Benzenemethanaminium, N-(3-aminopropyl...-aminopropyl)-N,N-dimethyl-, N-soya acyl derivs., chlorides. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified as benzenemethanaminium, N-(3-aminopropyl)-N,N...

  6. Structure-property studies of thermoplastic and thermosetting polyurethanes using palm and soya oils-based polyols.

    Science.gov (United States)

    Mohammed, Issam Ahmed; Al-Mulla, Emad Abbas Jaffar; Kadar, Nurul Khizien Abdul; Ibrahim, Mazlan

    2013-01-01

    Palm and soya oils were converted to monoglycerides via transesterification of triglycerides with glycerol by one step process to produce renewable polyols. Thermoplastic polyurethanes (TPPUs) were prepared from the reaction of the monoglycerides which act as polyol with 4,4'-methylenediphenyldiisocyanate (MDI) whereas, thermosetting polyurethanes (TSPUs) were prepared from the reaction of glycerol, MDI and monoglycerides in one pot. Characterization of the polyurethanes was carried out by FT-IR, (1)H NMR, and iodine value and sol-gel fraction. The TSPUs showed good thermal properties compared to TPPUs as well as TSPUs exhibits good properties in pencil hardness and adhesion, however poorer in flexural and impact strength compared to TPPUs. The higher percentage of cross linked fraction, the higher degree of cross linking occurred, which is due to the higher number of double bond presents in the TSPUs. These were reflected in iodine value test as the highest iodine value of the soya-based thermosetting polyurethanes confirmed the highest degree of cross linking. Polyurethanes based on soya oil showed better properties compared to palm oil. This study is a breakthrough development of polyurethane resins using palm and soya oils as one of the raw materials.

  7. Soya beans and maize : the effect of chemical and physical structure of cell wall polysaccharides on fermentation kinetics

    NARCIS (Netherlands)

    Laar, van H.

    2000-01-01

    The analysis of the relationship between cell wall composition and fermentation of endosperm cell walls of soya beans and maize was approached from three different angles. Firstly, the fermentation (rate and extent of fermentation, the sugar degradation pattern, and volatile fatty acid

  8. Monitoreo de arroz, soya y maíz comercializados en Nicaragua para detección de material transgénico

    Directory of Open Access Journals (Sweden)

    Julio A. Gómez-Rodríguez

    2007-12-01

    Full Text Available EL CULTIVO DE ORGANISMOS GENÉTICAMENTE MODIFICADOS –conocidos como transgénicos–ha ido en aumento desde su aprobación comercial en 1994. Nicaragua no tiene restricciones de importación de estas semillas y alimentos. Sin embargo, dado el intercambio comercial con países productores de transgénicos, se ha especulado que no se han respetado las normas de importación establecidas. El Centro de Biología Molecular de la UCA, a través de un muestreo al azar, se propuso investigar la presencia de material transgénico en arroz, soya y maíz importado y disponible en los principales puestos de venta del país. Analizamos las muestras mediante dos métodos comúnmente usados en laboratorios de diagnóstico molecular: pruebas inmunológicas y detección a nivel de ADN del promotor 35S y el terminador NOS. Aquí presentamos los resultados del trabajo y reportamos que en el período comprendido en este estudio no se detectaron semillas transgénicas en Nicaragua.

  9. Effect of Weather on the Occurrence of Puccinia Graminis Subsp. Graminicola and Puccinia Coronata F. Sp. Lolii at Lolium Perenne L. and Deschampsia Caespitosa (L. P. B.

    Directory of Open Access Journals (Sweden)

    Monika Novotná

    2017-01-01

    Full Text Available Monitoring of Puccinia graminis subsp. graminicola and Puccinia coronata f. sp. lolii was carried out in Plant breeding station called Větrov. The pathogens were estimated on turf grass (Lolium perenne L., Deschampsia caespitosa (L. P. B. from 2009 to 2014. Puccinia graminis subsp. graminicola was detected in the increased level in 2009 and 2012. The highest amount of mixed infections was determined in 2014 because of the warmest winter from all monitored years and low precipitations. Significant differences were found out in the resistance of similar plant materials grown in different fields. Significant effect of weather conditions and supposed effect of different infectious pressure on various fields were reflected in these facts. At evaluated grasses, the highest (P < 0.05 occurence of Puccinia graminis subsp. graminicola. Lolium perenne L. was observed and the infection of Puccinia graminis subsp. graminicola (P < 0.05 was determined higher than in Deschampsia caespitosa (L. P. B.

  10. Effect of soya milk on nutritive, antioxidative, reological and textural properties of chocolate produced in a ball mill

    Directory of Open Access Journals (Sweden)

    Zarić Danica B.

    2011-01-01

    Full Text Available Chocolate is a complex rheological system in which non-fat cocoa particles and sugar particles are enveloped by crystal matrix of cocoa butter. Physical properties of chocolate depend on ingredient composition, method of production and properly performed pre-crystallization phase. In this work, chocolate was produced in an unconventional way, i.e. in a ball mill applying variable refining time (30, 60 and 90 min and pre-crystallization temperature in chocolate masses (26, 28 and 30⁰C. Two types of chocolate were produced: chocolate with 20% of powdered cow’s milk (R1 and chocolate with 20% of soya milk powder (R2. The quality of chocolate was followed by comparing nutritive composition, 23 polyphenol content, hardness of chocolate, solid triglyceride content (SFC and rheological parameters (Casson yield flow (Pa, Casson viscosity (Pas, the area of the thixotropic loop, elastic modulus and creep curves. The aim of this paper is determining changes caused by replacing cow’s milk with soya milk powder in respect of nutritive, rheological and sensory properties, as well as defining optimal parameters: precrystallization temperature and refining time of soya milk chocolate in order to obtain suitable sensory and physical properties identical to those of milk chocolate mass. The results show that replacing powdered cow’s milk with soya milk powder affects the increase in nutritive value of soya milk chocolate. R2 chocolate mass showed the increase in essential amino acids and fatty acids. This chocolate mass also showed the increase in total polyphenols by 28.1% comparing to R1 chocolate mass. By comparing viscosity, yield stress, shear stress and the area of the thixotropic loop, it can be observed that R1 chocolate mass represents a more organized and simpler system than the mass with soya milk (R2, as it shows lower values for all the above mentioned parameters, regardless of the refining time and pre-crystallization temperature

  11. Nanoscale zero-valent iron assisted phytoremediation of Pb in sediment: Impacts on metal accumulation and antioxidative system of Lolium perenne.

    Science.gov (United States)

    Huang, Danlian; Qin, Xiang; Peng, Zhiwei; Liu, Yunguo; Gong, Xiaomin; Zeng, Guangming; Huang, Chao; Cheng, Min; Xue, Wenjing; Wang, Xi; Hu, Zhengxun

    2018-05-30

    Lead (Pb) is a highly toxic environmental pollutant, and could result in toxic effects on living organisms. The effects of 0, 100, 200, 500, 1000 and 2000 mg/kg of nZVI on plant growth, Pb accumulation and antioxidative responses of Lolium perenne were investigated. Results showed that the total Pb contents in L. perenne with the treatment of low concentrations of nZVI (100, 200 and 500 mg/kg) were higher than those in the non-nZVI treatments, and the highest Pb accumulation capacity of 1175.40 μg per pot was observed in L. perenne with the treatment of 100 mg/kg nZVI. However, the total Pb contents in L. perenne decreased at high concentrations of nZVI (1000 and 2000 mg/kg). This might be resulted from the decrease of photosynthetic chlorophyll content and the aggravated oxidative stress induced by the high concentration of nZVI, which caused the decrease of plant biomass and metal accumulation capacity in plant. Moreover, the sequential extraction experiments results showed that the lowest acid soluble fraction of Pb in the sediments was found in the treatment with 100 mg/kg of nZVI, indicating that 100 mg/kg was the optimum concentration for nZVI to assist the phytoremediation of Pb-polluted sediment. To conclude, these findings provide a promising method to remediate Pb-polluted sediment by nZVI assisted phytoremediation. Copyright © 2018 Elsevier Inc. All rights reserved.

  12. Effects of coal spoil amendment on heavy metal accumulation and physiological aspects of ryegrass (Lolium perenne L.) growing in copper mine tailings.

    Science.gov (United States)

    Chu, Zhaoxia; Wang, Xingming; Wang, Yunmin; Liu, Guijian; Dong, Zhongbing; Lu, Xianwen; Chen, Guangzhou; Zha, Fugeng

    2017-12-21

    Copper mine tailings pose many threats to the surrounding environment and human health, and thus, their remediation is fundamental. Coal spoil is the waste by-product of coal mining and characterized by low levels of metals, high content of organic matter, and many essential microelements. This study was designed to evaluate the role of coal spoil on heavy uptake and physiological responses of Lolium perenne L. grown in copper mine tailings amended with coal spoil at rates of 0, 0.5, 1, 5, 10, and 20%. The results showed that applying coal spoil to copper mine tailings decreased the diethylenetriaminepentaacetic acid (DTPA)-extractable Cd, Cu, Pb, and Zn contents in tailings and reduced those metal contents in both roots and shoots of the plant. However, application of coal spoil increased the DTPA-extractable Cr concentration in tailings and also increased Cr uptake and accumulation by Lolium perenne L. The statistical analysis of physiological parameters indicated that chlorophyll and carotenoid increased at the lower amendments of coal spoil followed by a decrease compared to their respective controls. Protein content was enhanced at all the coal spoil amendments. When treated with coal spoil, the activities of superoxide dismutases (SOD), peroxidase (POD), and catalase (CAT) responded differently. CAT activity was inhibited, but POD activity was increased with increasing amendment ratio of coal spoil. SOD activity increased up to 1% coal spoil followed by a decrease. Overall, the addition of coal spoil decreased the oxidative stress in Lolium perenne L., reflected by the reduction in malondialdehyde (MDA) contents in the plant. It is concluded that coal spoil has the potential to stabilize most metals studied in copper mine tailings and ameliorate the harmful effects in Lolium perenne L. through changing the physiological attributes of the plant grown in copper mine tailings.

  13. Evaluación del efecto del grano de soya crudo (C.S.C. suministrado por 42-28 o 14 semanas a aves en postura

    Directory of Open Access Journals (Sweden)

    López G. Arnobio

    1988-12-01

    Full Text Available An experiment was conducted with 144 middle weight Dekalb-Warren laying hens (28 to 70 weeks age, to evaluate the effects on feed consumption, % egg production, egg weight feed conversion per dozen and kilogram of egg produced, weight change, and pancreas size and weight due to the feeding of who le raw soybean (WRSB replacing soybean bean (SBM as a protein source in the diet in three feeding periods (42,28 and 14 weeks. There were no differences (P<0.05 due to protein sources used on egg weight, feed conversion per dozen and kilogram of egg produced and pancreas size. However, there were differences on egg production, weight change, feed consumption by hen per day and pancreas weight. Also there on differences (P < 0.05 due to feeding period on egg weight, feed conversion by dozen and kilograms of egg produced and pancreas weight, but there were differences (P<0.05 on egg production and pancreas size. In a similar way, there where no differences (P<0.05 to the interaction of protein sources feeding periods for any of the variables considerated.Un experimento se llevó a cabo con 144 gallinas Dekalb-Warren semipesadas en postura (28 a 70 semanas para evaluar los efectos de reemplazar la torta de soya por el grano de soya crudo (G.S.C suministrada por tres períodos de tiempo diferentes(42, 28 y 14 semanas. No hubo diferencias (P <0.05 por efecto de la fuente de proteína para las variables peso de huevo, eficiencia de conversión por docena y kilogramo de huevo producido y tamaño de pancreas. Sin embargo si hubo diferencias (P < 0.05 para el porcentaje de producción, cambio de peso en las aves, consumo de alimento por ave día y peso del páncreas. Tampoco se encontraron diferencias (P<0.05 por efecto del período de tiempo de suministro para las variables cambio de peso en las aves, peso de huevo, eficiencia de conversión por docena y kilogramo de huevo producido y peso del páncreas. Pero si hubo diferencias (P< Q05 para las variables

  14. Soya isoflavone consumption in relation to carotid intima-media thickness in Chinese equol excretors aged 40-65 years.

    Science.gov (United States)

    Cai, Yun; Guo, Kaiping; Chen, Chaogang; Wang, Ping; Zhang, Bo; Zhou, Quan; Mei, Fang; Su, Yixiang

    2012-11-14

    Previous studies have suggested that the daidzein metabolite equol rather than daidzein itself contributes to the beneficial effect of soya foods in the prevention of CVD. The aim of the present study is to examine the proportion of equol excretion in Chinese adults and compare plasma lipids and carotid artery intima-media thickness (IMT) between equol excretors and non-excretors, and to evaluate the effect of soya isoflavone intakes on serum lipids and IMT in either equol excretors or non-excretors. Subjects (n 572; women n 362, men n 210) were recruited for the present study. An overnight urine sample was provided by each subject on their usual diet to quantify urinary concentrations of daidzein and equol. Far-wall IMT was determined by B-mode ultrasound in the right carotid at two sites, carotid bulb (CB-IMT) and common carotid artery (CCA-IMT), and fasting serum lipids were measured. Habitual dietary intakes were estimated with a FFQ, and soya isoflavone intake derived from the FFQ was assessed. Of the 572 subjects, the proportion of equol excretors on their usual diet was 25·0 % (n 143). Compared with non-excretors, equol excretors showed significantly lower serum TAG (-38·2 (95 % CI -70·4, -5·9) %, P = 0·012) and CCA-IMT (-4·9 (95 % CI -9·7, -0·3) %, P = 0·033). Equol excretors with higher daily isoflavone intakes (-5·4 mg/d) had significantly lower IMT (-16·2 %, P = 0·035) and tended to have higher HDL-cholesterol (P = 0·055) than did those with lower daily isoflavone intakes (1·5 mg/d), while no association was observed between soya isoflavone intakes and serum lipids or IMT in non-excretors. In conclusion, the benefits of soya isoflavones in preventing CVD may be apparent among equol excretors only.

  15. Larvicultura de Rhamdia quelen (Pisces, Pimelodidae con proteína vegetal y animal, suplementadas con plancton

    Directory of Open Access Journals (Sweden)

    Germán Castañeda A.

    2011-12-01

    Full Text Available Objetivo. Determinar el efecto de dietas con diferentes proporciones de fuentes proteicas animales y vegetales suplementadas con plancton sobre el crecimiento y sobrevivencia en larvas de Rhamdia quelen. Materiales y métodos. Fueron formuladas dos dietas experimentales, dieta-1 con 70% de proteína de origen vegetal (torta de soya y dieta-2 con 70% de proteína proveniente de animales (corazón bovino yharina de pescado; se utilizaron cuatro protocolos de alimentación (cinco réplicas por tratamiento: dieta-1 + plancton filtrado en tamiz de 50-200 micras (T1, dieta-2 + plancton filtrado en tamiz de 50-200 micras (T2, dieta-1 (T3, dieta-2 (T4. Las larvas fueron manejadas a una densidad de 20 animales L-1 suministrando alimentohasta aparente saciedad diariamente a las 07:00, 11:00, 16:00 y 21:00 horas; la suplementación con plancton se realizó a las 11:00 y 21:00 horas. Resultados. Entre T1 y T2 no se observaron diferencias estadísticas (p>0.05 en peso final (21.89 ± 15.17mg vs 20.37 ± 10.37mg, longitud total (13.41±2.34mm vs 13.39±1.99 mm, factor de condición (K (0.80±0.13 vs 0.78±0.13 y sobrevivencia (46.6±2.68% vs36.0±7.41%; las diferencias entre T3 y T4 tampoco fueron significativas (3.35±1.40 mg vs 2.98±1.48 mg; 7.54±0.91mm vs 7.33±0.96mm; 0.75±0.13 vs 0.71±0.12; 33.6±9.07% vs 24.8±6.76%, respectivamente; hubo diferencias significativas (p<0.05 entre los grupos suplementados con plancton y los alimentados solo con ración. Conclusiones. La suplementación con plancton fue más efectiva que elofrecimiento solo de ración. La inclusión del 62.9% de torta de soya en la formulación (T1, aparentemente no afectó el crecimiento ni la sobrevivencia.

  16. An Epichloë festucae homologue of MOB3, a component of the STRIPAK complex, is required for the establishment of a mutualistic symbiotic interaction with Lolium perenne

    Science.gov (United States)

    Green, Kimberly A.; Becker, Yvonne; Fitzsimons, Helen L.

    2016-01-01

    Summary In both Sordaria macrospora and Neurospora crassa, components of the conserved STRIPAK (striatin‐interacting phosphatase and kinase) complex regulate cell–cell fusion, hyphal network development and fruiting body formation. Interestingly, a number of Epichloë festucae genes that are required for hyphal cell–cell fusion, such as noxA, noxR, proA, mpkA and mkkA, are also required for the establishment of a mutualistic symbiotic interaction with Lolium perenne. To determine whether MobC, a homologue of the STRIPAK complex component MOB3 in S. macrospora and N. crassa, is required for E. festucae hyphal fusion and symbiosis, a mobC deletion strain was generated. The ΔmobC mutant showed reduced rates of hyphal cell–cell fusion, formed intrahyphal hyphae and exhibited enhanced conidiation. Plants infected with ΔmobC were severely stunted. Hyphae of ΔmobC showed a proliferative pattern of growth within the leaves of Lolium perenne with increased colonization of the intercellular spaces and vascular bundles. Although hyphae were still able to form expressoria, structures allowing the colonization of the leaf surface, the frequency of formation was significantly reduced. Collectively, these results show that the STRIPAK component MobC is required for the establishment of a mutualistic symbiotic association between E. festucae and L. perenne, and plays an accessory role in the regulation of hyphal cell–cell fusion and expressorium development in E. festucae. PMID:27277141

  17. Complete primary structure of a Lolium perenne (perennial rye grass) pollen allergen, Lol p III: comparison with known Lol p I and II sequences.

    Science.gov (United States)

    Ansari, A A; Shenbagamurthi, P; Marsh, D G

    1989-10-17

    The complete amino acid sequence of a Lolium perenne (rye grass) pollen allergen, Lol p III, determined by the automated Edman degradation of the protein and its selected fragments, is reported in this paper. Cleavage by enzymatic and chemical techniques established unambiguously the sequence for this 97-residue protein (Mr = 10,909), which lacks cysteine and shows no evidence of glycosylation. The sequence of Lol p III is very similar to that of another L. perenne allergen, Lol p II, which was sequenced recently; of the 97 positions in the two proteins, 57 are occupied by identical amino acids (59% identity). In addition, both allergens share a similar structure with an antibody-binding fragment of a third L. perenne allergen, Lol p I. Since human antibody responsiveness to all these three allergens is associated with HLA-DR3, and since the structure common to the three molecules shows high degrees of amphipathicity in Lol p II and III, we speculate that this common segment in the three molecules might contain or contribute to the respectively Ia/T-cell sites.

  18. Genetic diversity and structure of Lolium perenne ssp. multiflorum in California vineyards and orchards indicate potential for spread of herbicide resistance via gene flow.

    Science.gov (United States)

    Karn, Elizabeth; Jasieniuk, Marie

    2017-07-01

    Management of agroecosystems with herbicides imposes strong selection pressures on weedy plants leading to the evolution of resistance against those herbicides. Resistance to glyphosate in populations of Lolium perenne L. ssp. multiflorum is increasingly common in California, USA, causing economic losses and the loss of effective management tools. To gain insights into the recent evolution of glyphosate resistance in L. perenne in perennial cropping systems of northwest California and to inform management, we investigated the frequency of glyphosate resistance and the genetic diversity and structure of 14 populations. The sampled populations contained frequencies of resistant plants ranging from 10% to 89%. Analyses of neutral genetic variation using microsatellite markers indicated very high genetic diversity within all populations regardless of resistance frequency. Genetic variation was distributed predominantly among individuals within populations rather than among populations or sampled counties, as would be expected for a wide-ranging outcrossing weed species. Bayesian clustering analysis provided evidence of population structuring with extensive admixture between two genetic clusters or gene pools. High genetic diversity and admixture, and low differentiation between populations, strongly suggest the potential for spread of resistance through gene flow and the need for management that limits seed and pollen dispersal in L. perenne .

  19. Neutron activation analysis of Standard Materials of Comparison IAEA- the corn and soya flour

    International Nuclear Information System (INIS)

    Dadakhanov, J.A.; Sadykov, I.I.; Salimov, M.I.

    2005-01-01

    It is known that maintenance of quality of results of neutron activation analysis (NAA), no less than in other analytical methods, is one of key problems. Thus first of all it is necessary to provide correctness of results. The most correct way of revealing and elimination of regular errors is carrying out of analyses of Standard Samples of Comparison (SSC) by the developed techniques and comparison of the received results with the certificated results. Therefore, the analysis and certification of various SSC is one of the most actual tasks of modern analytical chemistry. One of few organizations engaged in manufacture SSC for NAA, is IAEA which has organized the Project on certification of samples of comparison - a corn and soya flour. Among many laboratories worldwide, in this Project the Laboratory of the Activation Analysis of Pure Materials of Institute of Nuclear Physics Academy of Sciences Republic of Uzbekistan also participated. We carry out series of analyses of samples of corn and soya flour, candidates for standard samples of comparison, by the method of instrumental NAA. The preparing of samples was carried out by the technique described in the technical project applied to these materials. Samples before the analysis dried up at temperature 80 degree C during 24 h. Cooled, weighed and irradiated in the vertical channel of a nuclear reactor of VVR-SM of Institute of Nuclear Physics Academy of Sciences Republic of Uzbekistan during 0,5-1 h (depending on determined elements) with density of a stream of neutrons 1 x 10 14 neutrons/cm 2 sec. Time of cooling from 10 min up to 10 days. Time of measurement from 100 sec up to 3000 sec. Measurements were carried out on gamma spectrometer consisting of HPGe detector GC1518 and digital multichannel analyzer DSA-1000('Canberra', USA). Processing of the spectrometer information carried out with the help of software package Genie-2000. As a result of the carried out analyses we determine contents of 21 elements in

  20. Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.

    Directory of Open Access Journals (Sweden)

    Thrush Anthony

    2010-01-01

    Full Text Available Abstract Background Perennial ryegrass (Lolium perenne L. is an important pasture and turf crop. Biotechniques such as gene expression studies are being employed to improve traits in this temperate grass. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR is among the best methods available for determining changes in gene expression. Before analysis of target gene expression, it is essential to select an appropriate normalisation strategy to control for non-specific variation between samples. Reference genes that have stable expression at different biological and physiological states can be effectively used for normalisation; however, their expression stability must be validated before use. Results Existing Serial Analysis of Gene Expression data were queried to identify six moderately expressed genes that had relatively stable gene expression throughout the year. These six candidate reference genes (eukaryotic elongation factor 1 alpha, eEF1A; TAT-binding protein homolog 1, TBP-1; eukaryotic translation initiation factor 4 alpha, eIF4A; YT521-B-like protein family protein, YT521-B; histone 3, H3; ubiquitin-conjugating enzyme, E2 were validated for qRT-PCR normalisation in 442 diverse perennial ryegrass (Lolium perenne L. samples sourced from field- and laboratory-grown plants under a wide range of experimental conditions. Eukaryotic EF1A is encoded by members of a multigene family exhibiting differential expression and necessitated the expression analysis of different eEF1A encoding genes; a highly expressed eEF1A (h, a moderately, but stably expressed eEF1A (s, and combined expression of multigene eEF1A (m. NormFinder identified eEF1A (s and YT521-B as the best combination of two genes for normalisation of gene expression data in perennial ryegrass following different defoliation management in the field. Conclusions This study is unique in the magnitude of samples tested with the inclusion of numerous field-grown samples

  1. Eriophorum angustifolium and Lolium perenne metabolic adaptations to metals- and metalloids-induced anomalies in the vicinity of a chemical industrial complex.

    Science.gov (United States)

    Anjum, Naser A; Ahmad, Iqbal; Rodrigues, Sónia M; Henriques, Bruno; Cruz, Nuno; Coelho, Cláudia; Pacheco, Mário; Duarte, Armando C; Pereira, Eduarda

    2013-01-01

    As plants constitute the foundation of the food chain, concerns have been raised about the possibility of toxic concentrations of metals and metalloids being transported from plants to the higher food chain strata. In this perspective, the use of important phytotoxicity endpoints may be of utmost significance in assessing the hazardous nature of metals and metalloids and also in developing ecological soil screening levels. The current study aimed to investigate the role of glutathione (GSH) and its associated enzymes in the metabolic adaptation of two grass species namely Eriophorum angustifolium Honck. and Lolium perenne L. to metals and metalloids stress in the vicinity of a chemical industrial complex (Estarreja, Portugal). Soil and plant samples were collected from contaminated (C) and non-contaminated (reference, R) sites, respectively, near and away from the Estarreja Chemical Complex, Portugal. Soils (from 0 to 10 and 10 to 20 cm depths) were analyzed for pH, organic carbon, and metals and metalloids concentrations. Plant samples were processed fresh for physiological and biochemical estimations, while oven-dried plant samples were used for metals and metalloids determinations following standard methodologies. Both soils and plants from the industrial area exhibited differential concentrations of major metals and metalloids including As, Cu, Hg, Pb, and Zn. In particular, L. perenne shoot displayed significantly higher and lower concentrations of Pb and As, respectively at contaminated site (vs. E. angustifolium). Irrespective of sites, L. perenne shoot exhibited significantly higher total GSH pool, oxidized glutathione (GSSG) and oxidized protein (vs. E. angustifolium). Additionally, severe damages to photosynthetic pigments, proteins, cellular membrane integrity (in terms of electrolyte leakage), and lipid peroxidation were also perceptible in L. perenne shoot. Contrarily, irrespective of the sites, activities of catalase and GSH-regenerating enzyme, GSH

  2. Evaluación de arroz Paddy y soya integral cocida en la alimentación de pollas de cría y levante (0-18 semanas

    Directory of Open Access Journals (Sweden)

    Santos V. Eduardo

    1989-06-01

    Full Text Available El experimento se realizó con pollas livianas y semipesadas, en las etapas de cría y levante (0-8 y 9-18 semanas con el fin de evaluar tres niveles de reemplazo de sorgo por arroz paddy (0,50 Y 100% en la dieta, en combinación con torta de soya o soya integral cocida (SIC, con un arreglo factorial 3 x 2 x 2 correspondiente a 12 tratamientos con diferente número de replicaciones y 32 unidades experimentales de 56 y 49 aves respectivamente para las líneas liviana y semipesada. Se emplearon 251 pollas de la línea Dekalb-XL-Link y 220 de la línea Dekalb Warren para evaluar el efecto de las dietas de cría y levante, hasta el pico de postura. No hubo diferencias (P<0.05 en consumo de alimento, aumento de peso y conversión alimenticia durante la fase de cría, debidas al reemplazo de sorgo por arroz paddy. En la fase de levante hubo diferencias (P < 0.05 en consumo de alimento y conversión alimenticia por efecto de los niveles de arroz paddy en La dieta. En la fase de postura no se presentaron diferencias (P < 0.05 en el desarrollo fisiológico y rendimiento productivo atribuibles al efecto de las dietas. La mejor respuesta biológica y económica (fase de cría, levante y postura se alcanzó con soya integral cocida como fuente de proteína.An experiment was conducted with light and double purpose strains of growing-rearing pullets (0-8 and 9-18 weeks age, to evaluate the effects of the replacement (0,50 and 100% of milo by paddy rice with whole cooked soybean (WCSB and soybean meal (SBM with a arrangement (3 x 2 x 2 for a total of twelve treatment and 32 experimental units of 56 and 49 light and double purpose pullets respectively. 251 light and 220 double purpose pullets were use to evaluate the effects of the growing-rearing diets phase on the laying phase. There were no differences (P<0.05 in feed consumption, weight gain, and feed efficiency during the growing phase, produced by the paddy rice levels used in the diet. In the rearing

  3. The Inhibitive Action Of Sodium Soya Sulphonate Towards The Corrosion Of Aluminium In Hydrochloric Acid

    OpenAIRE

    Mourad, M. Y.; Mead, A. I.; Seliman, S. A.

    1993-01-01

    The dissolution of aluminium in 2M hydrochloric acid in the presence of sodium soya sulphonate (SSS) as corrosion inhibitor has been studied using hydrogen evolution and thermometric methods. The two methods gave consistent results. The results obtained indicate that the inhibitive effect of the sulphonated mixture relates to chemisorption mechanism on the metal surface via the n electrons in the double bonds. تمت دراسة ذوبان الألمونيوم في محلول 2 مولارى من حمض الهيدروكلوريك في وجود سلفونا...

  4. Comparative metabolite fingerprinting of the rumen system during colonisation of three forage grass (Lolium perenne L. varieties.

    Directory of Open Access Journals (Sweden)

    Alison H Kingston-Smith

    Full Text Available The rumen microbiota enable ruminants to degrade complex ligno-cellulosic compounds to produce high quality protein for human consumption. However, enteric fermentation by domestic ruminants generates negative by-products: greenhouse gases (methane and environmental nitrogen pollution. The current lack of cultured isolates representative of the totality of rumen microbial species creates an information gap about the in vivo function of the rumen microbiota and limits our ability to apply predictive biology for improvement of feed for ruminants. In this work we took a whole ecosystem approach to understanding how the metabolism of the microbial population responds to introduction of its substrate. Fourier Transform Infra Red (FTIR spectroscopy-based metabolite fingerprinting was used to discriminate differences in the plant-microbial interactome of the rumen when using three forage grass varieties (Lolium perenne L. cv AberDart, AberMagic and Premium as substrates for microbial colonisation and fermentation. Specific examination of spectral regions associated with fatty acids, amides, sugars and alkanes indicated that although the three forages were apparently similar by traditional nutritional analysis, patterns of metabolite flux within the plant-microbial interactome were distinct and plant genotype dependent. Thus, the utilisation pattern of forage nutrients by the rumen microbiota can be influenced by subtleties determined by forage genotypes. These data suggest that our interactomic approach represents an important means to improve forages and ultimately the livestock environment.

  5. Allelopathic Potential of Switchgrass ( Panicum virgatum L.) on Perennial Ryegrass ( Lolium perenne L.) and Alfalfa ( Medicago sativa L.)

    Science.gov (United States)

    Shui, Junfeng; An, Yu; Ma, Yongqing; Ichizen, Nobumasa

    2010-10-01

    This study investigated allelopathy and its chemical basis in nine switchgrass ( Panicum virgatum L.) accessions. Perennial ryegrass ( Lolium perenne L.) and alfalfa ( Medicago sativa L.) were used as test species. Undiluted aqueous extracts (5 g plant tissue in 50 ml water) from the shoots and roots of most of the switchgrass accessions inhibited the germination and growth of the test species. However, the allelopathic effect of switchgrass declined when extracts were diluted 5- or 50-fold. Seedling growth was more sensitive than seed germination as an indicator of allelopathic effect. Allelopathic effect was related to switchgrass ecotype but not related to ploidy level. Upland accessions displayed stronger allelopathic potential than lowland accessions. The aqueous extract from one switchgrass accession was separated into phenols, organic acids, neutral chemicals, and alkaloids, and then these fractions were bioassayed to test for allelopathic potential. Alkaloids had the strongest allelopathic effect among the four chemical fractions. In summary, the results indicated that switchgrass has allelopathic potential; however, there is not enough evidence to conclude that allelopathic advantage is the main factor that has contributed to the successful establishment of switchgrass on China’s Loess Plateau.

  6. Arsenic extractability and uptake by velvetgrass Holcus lanatus and ryegrass Lolium perenne in variously treated soils polluted by tailing spills.

    Science.gov (United States)

    Karczewska, Anna; Lewińska, Karolina; Gałka, Bernard

    2013-11-15

    Phytostabilization should be considered as an appropriate phytoremediation technique to restore the area affected by tailing spills in Zloty Stok, where arsenic ores were mined and processed for several centuries. The study aimed to compare the suitability of velvetgrass (Holcus lanatus L.) and ryegrass (Lolium perenne L.) for development of plant cover in that area. Various treatments commonly applied to support phytostabilization were examined. A pot experiment was carried out to assess the effects of soil amendment with phosphate (P), sewage sludge (SS) and iron salts (Fe) on arsenic extractability and its uptake by grass. Four kinds of soil material, containing 356-5350 mg kg(-1) As, were examined. Velvetgrass proved to be more resistant than ryegrass to the toxicity of soil arsenic. Ammonium sulphate extractability of As in soils correlated well with As concentrations in the biomass of both grass species. In three of four tested soils, application of Fe failed to decrease As extractability and to reduce its concentrations in the aboveground parts of grasses. Application of P and SS resulted in increased As solubility in soils, but their effects on plant biomass and As uptake were ambiguous. SS had a strong beneficial influence on the growth of velvetgrass, while such an effect was not observed for ryegrass. Copyright © 2012 Elsevier B.V. All rights reserved.

  7. Physiological effects of magnetite (Fe3O4) nanoparticles on perennial ryegrass (Lolium perenne L.) and pumpkin (Cucurbita mixta) plants.

    Science.gov (United States)

    Wang, Huanhua; Kou, Xiaoming; Pei, Zhiguo; Xiao, John Q; Shan, Xiaoquan; Xing, Baoshan

    2011-03-01

    To date, knowledge gaps and associated uncertainties remain unaddressed on the effects of nanoparticles (NPs) on plants. This study was focused on revealing some of the physiological effects of magnetite (Fe(3)O(4)) NPs on perennial ryegrass (Lolium perenne L.) and pumpkin (Cucurbita mixta cv. white cushaw) plants under hydroponic conditions. This study for the first time reports that Fe(3)O(4) NPs often induced more oxidative stress than Fe(3)O(4) bulk particles in the ryegrass and pumpkin roots and shoots as indicated by significantly increased: (i) superoxide dismutase and catalase enzyme activities, and (ii) lipid peroxidation. However, tested Fe(3)O(4) NPs appear unable to be translocated in the ryegrass and pumpkin plants. This was supported by the following data: (i) No magnetization was detected in the shoots of either plant treated with 30, 100 and 500 mg l(-1) Fe(3)O(4) NPs; (ii) Fe K-edge X-ray absorption spectroscopic study confirmed that the coordination environment of Fe in these plant shoots was similar to that of Fe-citrate complexes, but not to that of Fe(3)O(4) NPs; and (iii) total Fe content in the ryegrass and pumpkin shoots treated with Fe(3)O(4) NPs was not significantly increased compared to that in the control shoots.

  8. Estudio DRX en Materiales Catalizadores Cu-Ni-SiO2 para Hidrogenación de aceite de soya. Desarrollo de estructura cristalina y actividad catalítica

    Directory of Open Access Journals (Sweden)

    Gloria I. Cubillos

    2009-01-01

    Full Text Available Para buscar elementos experimentales que soporten la hipótesis según la cual se forman estructuras cristalinas Cu-Ni o eventualmente Cu-Ni-SiO2 en la síntesis de catalizadores para la hidrogenación de aceite de soya, se prepararon mezclas de estos tres materiales a partir de sales de los metales con sílice (aerosil. Se calcinaron a 270oC y se redujeron a 330oC en atmósfera de hidrógeno. En  los materiales reducidos se  estudió el difractógrama de RX en el rango 37 a 53 grados, y se comparó con el difractógrama de aleaciones Cu-Ni, en las cuales se conoce la formación  de cristales mixtos, y  con los difractógramas de los elementos puros Cu y Ni.  Los resultados muestran que se presentan señales de difracción diferentes a las señales de los componentes puros. La actividad catalítica se muestra diferente en los materiales que presentan distorsión en su estructura cristalina.  

  9. No direct effects of resistant soybean cultiva IAC-24 on Podisus nigrispinus (Heteroptera: Pentatomidae Efectos no directos de resistencia del cultivar de soya IAC-24 sobre Podisus nigrispinus (Heteroptera: Pentatomidae

    Directory of Open Access Journals (Sweden)

    José Cola Zanuncio

    2012-12-01

    'IAC-24' redujo la duración del IV estado de este depredador. La supervivencia de P. nìgrìspìnus fue similar entre los tratamientos en los estados I, II, IV y V y en el estado de ninfa, la supervivencia del estado III fue mayor con las plantas 'IAC-24'. La masa corporal de las ninfas en el primer día, en los estados III, IV, y V después de la ecdisis y adultos recién emergidos; características reproductivas (períodos de pre-oviposición, oviposición, post-oviposición e incubación de los huevos; intervalo de colocación de los huevos, número de masa de huevos por hembra, ninfas por hembra, ninfas por masa de huevos y porcentaje de eclosión de ninfas y vida útil de machos y hembras de P. nìgrìspìnus fueron similares entre los tratamientos. La soya resistente 'IAC-24' no mostró efectos nocivos directos en P. nìgrìspìnus, lo que implica su inocuidad y compatibilidad con este depredador.

  10. Modulation of mammary gland development in pre-pubertal mice as affected by soya and milk protein supplements.

    Science.gov (United States)

    Alston-Mills, Brenda; Lepri, J J; Martin, C A

    2011-08-01

    The objective of the present study was to determine the effects of soya and whey milk protein, α-lactalbumin (α-LA), on mammary gland morphology and the structural support of the gland, in pre-pubertal mice after 7 d of treatment. In Expt 1, weaned (day 21) CD1 mice were given one of the four treatments, three included dietary supplements: (1) control diet, casein, (2) soya, (3) α-LA and (4) subcutaneous injection of 2·5 μg oestradiol benzoate in 20 μl maize oil and fed the control diet. All diets were isoenergetic with equal protein concentrations. All groups that were not treated with oestradiol received the vehicle. Whole-mount analyses were performed to determine longitudinal ductal growth and terminal end bud development. DNA was extracted from the gland and assessed by spectrophotometry (260/280 nm). Tissue extracts for extracellular matrix (ECM) proteins, matrix metalloproteinase-2 (MMP(2)), tissue inhibitor of MMP(2) (TIMP(2)), and serum oestradiol and mammary tissue epidermal growth factors (EGF) were measured by immunoassays. Expt 2 utilised the Her2/neu transgenic strain, with the same protocols. Statistical significance was determined by one-way ANOVA. From Expt 1 and 2, soya and α-LA significantly increased ductal elongation when compared with the oestrogen and control groups. These results were corroborated by data on total DNA and the ratio of MMP(2):TIMP(2). The ratio of MMP(2):TIMP(2) was affected by α-LA. Serum oestradiol was decreased only in the oestradiol-treated groups in both experiments. Soya is known to be oestrogenic and can act on epithelia directly. The mechanism by which α-LA affects glandular development is by modulating the ECM or by promoting the synthesis/activity of EGF.

  11. A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis.

    Science.gov (United States)

    King, Julie; Thomas, Ann; James, Caron; King, Ian; Armstead, Ian

    2013-07-03

    Ryegrasses and fescues (genera, Lolium and Festuca) are species of forage and turf grasses which are used widely in agricultural and amenity situations. They are classified within the sub-family Pooideae and so are closely related to Brachypodium distachyon, wheat, barley, rye and oats. Recently, a DArT array has been developed which can be used in generating marker and mapping information for ryegrasses and fescues. This represents a potential common marker set for ryegrass and fescue researchers which can be linked through to comparative genomic information for the grasses. A F2 perennial ryegrass genetic map was developed consisting of 7 linkage groups defined by 1316 markers and deriving a total map length of 683 cM. The marker set included 866 DArT and 315 gene sequence-based markers. Comparison with previous DArT mapping studies in perennial and Italian ryegrass (L. multiflorum) identified 87 and 105 DArT markers in common, respectively, of which 94% and 87% mapped to homoeologous linkage groups. A similar comparison with meadow fescue (F. pratensis) identified only 28 DArT markers in common, of which c. 50% mapped to non-homoelogous linkage groups. In L. perenne, the genetic distance spanned by the DArT markers encompassed the majority of the regions that could be described in terms of comparative genomic relationships with rice, Brachypodium distachyon, and Sorghum bicolor. DArT markers are likely to be a useful common marker resource for ryegrasses and fescues, though the success in aligning different populations through the mapping of common markers will be influenced by degrees of population interrelatedness. The detailed mapping of DArT and gene-based markers in this study potentially allows comparative relationships to be derived in future mapping populations characterised using solely DArT markers.

  12. Efectividad de la mezcla picloram y fluroxipir en el control de malezas perennes de pastizales tropicales

    Directory of Open Access Journals (Sweden)

    Valent\\u00EDn A. Esqueda Esquivel

    2005-01-01

    Full Text Available Se determinóla cinética de la degradación ruminal de la materia seca, laproteína cruda y el extracto etéreo en las harinas de pescado,carne y hueso y tortave. Con base en los resultados de degradación a 48 horas de incubación ruminal, se determinó enforma biológica los contenidos digeribles reales de la proteí-na y grasa digerida, haciendo más precisa la medición del total de nutrientes digeribles y los valores de energía digestibley neta de los alimentos que por el método químico. Los valores de energía neta para producción fueron de 2,02, 1,66 y2,60 Mcals/kg en la harina de pescado, harina de carne y hue-so y la tortave, respectivamente. La fracción de proteína soluble (A fue alta en la harina de pescado (75,29%, media enla harina de carne y hueso (46,83% y más baja en la harinade tortave (25,75%, con una fracción degradable (B de10,62, 27,66 y 42,06%; así como una fracción insoluble (Cde 14,09, 25,51, y 32,19%, respectivamente. La proteína de-gradada en el rumen fue de 83,23, 66,25 y 51,98%, en el mismo orden de presentación anterior. Los contenidos de proteína cruda efectivamente retenida por el animal durante elproceso de digestión total, fueron de 53,29, 51,86 y 42,05%para la harina de pescado, harina de carne y hueso y la harina de tortave.

  13. Casein and soya-bean protein have different effects on whole body protein turnover at the same nitrogen balance

    DEFF Research Database (Denmark)

    Nielsen, K; Kondrup, J; Elsner, Petteri

    1994-01-01

    , or hydrolysed soya-bean protein at a level of 9.1 g/kg BW per d. The diets, which were isoenergetic with the same carbohydrate: fat ratio, were given as a continuous intragastric infusion for at least 4 d. During the last 19 h 15N-glycine (a primed continuous infusion) was given intragastrically and 15N...... synthesis. The protein diets produced a positive N balance which was independent of the protein source. Intact and hydrolysed casein increased protein synthesis 2.6- and 2.0-fold respectively, compared with the protein-free diet. Protein degradation increased 1.4- and 1.2-fold respectively. Hydrolysed soya-bean...... protein did not increase protein synthesis but decreased protein degradation by 35% compared with the protein-free diet. Compared with the hydrolysed soya-bean protein, intact casein resulted in 2.2- and 2.8-fold higher rates of protein synthesis and degradation respectively. These results are not easily...

  14. Relation of soya bean meal level to the concentration of plasma free amino acids and body growth in white rats.

    Science.gov (United States)

    Mandal, Tapas K; Parvin, Nargish; Mondal, Santanu; Saxena, Vijaylaxmi; Saxena, Ashok K; Sarkar, Sabyasachi; Saha, Mitali

    2012-04-01

    Amino acid (AA) levels in plasma and body growth were determined in rats (n20) fed diets with different soya bean meal levels. Free AA in plasma was determined by reversed-phase high-pressure liquid chromatography. We have used four levels of protein diets like 8%, 15%, 23% and 35% in this trial. Rats which were fed the low-protein (8%) diet with low percentage of soya bean meal were found to be growth-retarded. The body weight gain of high protein group (35%) was lower than that of the 23% groups. In the rats fed with the low-soya bean meal diet, some nonessential AA (NEAA) in plasma like asparagine, aspartic acid, cysteine, glutamic acid and serine increased, whereas the essential AA (EAA), with the exception of arginine, methionine and valine decreased. Here, plasma EAA-to-NEAA ratios were not correlated to growth and experimental diet. We hypothesize that AA metabolism is associated to changes in growth in rats on different protein intake. This study has showed the sensitivity of body mass gain, feed intake, feed conversion rate of rats to four levels of protein in the diet under controlled experimental conditions. © 2011 Blackwell Verlag GmbH.

  15. Soya Fasulyesi Ürünleri ile Beslenme Hedeflerine Ulaşılması (İngilizce

    Directory of Open Access Journals (Sweden)

    Levent Bayındırlı

    2015-02-01

    Full Text Available Genelde, hayvansal kaynaklı proteinler elzem amino asitlerin tümünü içerirken, bitkisel kaynaklı olanlar bu amino asitlerin bir veya birkaçından yoksundurlar. Soya proteinleri ise hayvansal kaynaklı proteinlerin besleyici değerini taşımakla birlikte daha ucuz fiyatlarla tüketime sunulması mümkündür. Bu çalışmada soya fasulyesinin gıda sistemlerindeki rolü, beslenme açısından bebeklerin, çocukların ve yaşlıların beslenmesinde; damar sertliği ve diğer bazı durumlarda kullanılması ile bu konudaki olası gelecek araştırmaların gereği tartışılmıştır. Dolayısıyla, dünyada proteine olan ihtiyaç ve bu ihtiyacın soya ürünleri ile karşılanabilmesi vurgulanmaya çalışılmıştır.

  16. Improving nutritional quality and fungal tolerance in soya bean and grass pea by expressing an oxalate decarboxylase.

    Science.gov (United States)

    Kumar, Vinay; Chattopadhyay, Arnab; Ghosh, Sumit; Irfan, Mohammad; Chakraborty, Niranjan; Chakraborty, Subhra; Datta, Asis

    2016-06-01

    Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known precursor of β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP), a neurotoxin found in grass pea. Here, we report the reduction in OA level in soya bean (up to 73%) and grass pea (up to 75%) seeds by constitutive and/or seed-specific expression of an oxalate-degrading enzyme, oxalate decarboxylase (FvOXDC) of Flammulina velutipes. In addition, β-ODAP level of grass pea seeds was also reduced up to 73%. Reduced OA content was interrelated with the associated increase in seeds micronutrients such as calcium, iron and zinc. Moreover, constitutive expression of FvOXDC led to improved tolerance to the fungal pathogen Sclerotinia sclerotiorum that requires OA during host colonization. Importantly, FvOXDC-expressing soya bean and grass pea plants were similar to the wild type with respect to the morphology and photosynthetic rates, and seed protein pool remained unaltered as revealed by the comparative proteomic analysis. Taken together, these results demonstrated improved seed quality and tolerance to the fungal pathogen in two important legume crops, by the expression of an oxalate-degrading enzyme. © 2016 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  17. Quantification of genetically modified soya using strong anion exchange chromatography and time-of-flight mass spectrometry.

    Science.gov (United States)

    Chang, Po-Chih; Reddy, P Muralidhar; Ho, Yen-Peng

    2014-09-01

    Stable-isotope dimethyl labeling was applied to the quantification of genetically modified (GM) soya. The herbicide-resistant gene-related protein 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) was labeled using a dimethyl labeling reagent, formaldehyde-H2 or -D2. The identification and quantification of CP4 EPSPS was performed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The CP4 EPSPS protein was separated from high abundance proteins using strong anion exchange chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Then, the tryptic peptides from the samples and reference were labeled with formaldehyde-H2 and formaldehyde-D2, respectively. The two labeled pools were mixed and analyzed using MALDI-MS. The data showed a good correlation between the peak ratio of the H- and D-labeled peptides and the GM soya percentages at 0.5, 1, 3, and 5 %, with R (2) of 0.99. The labeling reagents are readily available. The labeling experiments and the detection procedures are simple. The approach is useful for the quantification of GM soya at a level as low as 0.5 %.

  18. Identifikasi Gen Transgenik pada Produk Susu Bubuk Kedelai dan Susu Formula Soya dengan Metode PCR (Polymerase Chain Reaction

    Directory of Open Access Journals (Sweden)

    Agustin Krisna Wardani

    2018-01-01

      Kebutuhan kedelai yang mencapai 2,2 juta ton/tahun memaksa Indonesia mengimpor sebanyak 1,62 juta ton. Sebagian besar kedelai impor berupa kedelai transgenik. Dengan munculnya kedelai transgenik di Indonesia, perlu adanya pelabelan Produk Rekayasa Genetika (PRG untuk memenuhi hak-hak konsumen. Teknik yang dilakukan untuk mendeteksi PRG salah satunya menggunakan metode PCR. Penelitian ini bertujuan untuk mengetahui ada tidaknya gen transgenik pada produk susu bubuk kedelai dan formula soya, sehingga produk dapat digolongkan sebagai PRG atau tidak. Selain itu juga bertujuan untuk mengetahui suhu annealing optimum pada primer yang digunakan. Hasil penelitian didapatkan suhu annealing optimum primer CaMV 35S promotor adalah 60oC. Sedangkan untuk primer gen EPSPS-CP4 suhu annealing optimumnya 59oC. Untuk primer NOS terminator suhu annealing optimum tidak ditemukan. Dari amplifikasi DNA sampel, 6 sampel susu bubuk kedelai dan 5 sampel formula soya terdapat sisipan gen EPSPS-CP4 dan gen Promotor CaMV 35S. Dengan demikian 11 sampel tersebut dapat dikatakan sebagai PRG.   Kata kunci: Produk rekayasa genetika; PCR; formula soya; susu kedelai bubuk; kedelai transgenik

  19. Olivine weathering in soil, and its effects on growth and nutrient uptake in Ryegrass (Lolium perenne L.: a pot experiment.

    Directory of Open Access Journals (Sweden)

    Hein F M ten Berge

    Full Text Available Mineral carbonation of basic silicate minerals regulates atmospheric CO(2 on geological time scales by locking up carbon. Mining and spreading onto the earth's surface of fast-weathering silicates, such as olivine, has been proposed to speed up this natural CO(2 sequestration ('enhanced weathering'. While agriculture may offer an existing infrastructure, weathering rate and impacts on soil and plant are largely unknown. Our objectives were to assess weathering of olivine in soil, and its effects on plant growth and nutrient uptake. In a pot experiment with perennial ryegrass (Lolium perenne L., weathering during 32 weeks was inferred from bioavailability of magnesium (Mg in soil and plant. Olivine doses were equivalent to 1630 (OLIV1, 8150, 40700 and 204000 (OLIV4 kg ha(-1. Alternatively, the soluble Mg salt kieserite was applied for reference. Olivine increased plant growth (+15.6% and plant K concentration (+16.5% in OLIV4. At all doses, olivine increased bioavailability of Mg and Ni in soil, as well as uptake of Mg, Si and Ni in plants. Olivine suppressed Ca uptake. Weathering estimated from a Mg balance was equivalent to 240 kg ha(-1 (14.8% of dose, OLIV1 to 2240 kg ha(-1 (1.1%, OLIV4. This corresponds to gross CO(2 sequestration of 290 to 2690 kg ha(-1 (29 10(3 to 269 10(3 kg km(-2. Alternatively, weathering estimated from similarity with kieserite treatments ranged from 13% to 58% for OLIV1. The Olsen model for olivine carbonation predicted 4.0% to 9.0% weathering for our case, independent of olivine dose. Our % values observed at high doses were smaller than this, suggesting negative feedbacks in soil. Yet, weathering appears fast enough to support the 'enhanced weathering' concept. In agriculture, olivine doses must remain within limits to avoid imbalances in plant nutrition, notably at low Ca availability; and to avoid Ni accumulation in soil and crop.

  20. Evaluación de soya integral cocida y arroz paddy en la alimentación de pollas de levante

    Directory of Open Access Journals (Sweden)

    Avila G. Victoria

    1987-06-01

    Full Text Available

    A experiment was conducted to evaluate the effect on growing pullets  (9 to 18 weeks and from this age to peak produc­tion due to the replacement of 0, 50 and 100 % milo by paddy rice in combina­tion with soybean meal (SBM or whole cooked  soybean (WCSB in the diet. De­kalb Warren pullets alloted with a comple­tely randomized design and factorial arrangement 2x3, with 5 replications by treatment and 30 experimental units of 60 pullets each were used. A 15 % of the pullets of each replications were used to evaluate the effects of the 3 pro­duction phase. The statistical   analysis showed differences (P < 0.01 in feed consumption, weight gain and feed effi­ciency due to the replacement of milo and to the protein source used. Paddy ri­ce and WCSB can respectively replace 100 %  milo and SBM in growing    pu­llets diet. There were differences (P < 0.05 in sexual and peak production but not in 50 % production age due to the replacement of milo, decreasing the age to sexual maturity and increasing to peak production. Also, there were differences (P <0.05 in age to sexual maturity, 50% and peak production due to the protein source with an earlier age to se­xual and 50 o/o production age but la­test age to peak production by the   use of WCSB. The best alternative for Cauca Valley conditions was 100 % paddy ri­ce + WCSB

    En la dieta de pollas semipesadas, de la línea Dekalb Warren, de 9 a 18 semanas, se reemplazó el sorgo por arroz paddy (0,50 y 100% y se combinó con torta de so­ya ó soya integral cocida (SIC (100° C x 35 minutos. Las aves se distribuyeron me­diante un diseño completamente al azar y un arreglo factorial 3x2, los tratamientos se aplicaron 5 veces y 60 aves confor­maron cada unidad experimental. En el 15 % de las aves de cada replicación se evaluó el efecto hasta el pico de postura. En la fase de levante hubo diferencias (P < 0.01 en consumo, aumento de peso y conversión alimenticia. El arroz paddy

  1. Comparative sequence analysis of VRN1 alleles of Lolium perenne with the co-linear regions in barley, wheat, and rice

    DEFF Research Database (Denmark)

    Asp, Torben; Byrne, Stephen; Gundlach, Heidrun

    2011-01-01

    Vernalization, a period of low temperature to induce transition from vegetative to reproductive state, is an important environmental stimulus for many cool season grasses. A key gene in the vernalization pathway in grasses is the VRN1 gene. The objective of this study was to identify causative...... polymorphism(s) at the VRN1 locus in perennial ryegrass (Lolium perenne) for variation in vernalization requirement. Two allelic Bacterial Artificial Chromosome clones of the VRN1 locus from the two genotypes Veyo and Falster with contrasting vernalization requirements were identified, sequenced...

  2. Immunological cross-reactivity of the major allergen from perennial ryegrass (Lolium perenne), Lol p I, and the cysteine proteinase, bromelain.

    Science.gov (United States)

    Pike, R N; Bagarozzi, D; Travis, J

    1997-04-01

    Antibodies prepared in rabbits against the major allergen from ryegrass (Lolium perenne), Lol p I, cross-reacted with the cysteine proteinase bromelain from pineapple and vice versa. Deglycosylation of the proteins showed that the cross-reaction was based on recognition of the carbohydrate moiety of the allergen, but for bromelain the cross-reaction was most likely due to a combination of factors. The results indicate that the carbohydrate residues from these allergens play an important role in cross-reactions found between them and possibly those from other species.

  3. Effects of the duration and inorganic nitrogen composition of a nutrient-rich patch on soil exploration by the roots of Lolium perenne in a heterogeneous environment.

    Science.gov (United States)

    Nakamura, Ryoji; Kachi, N; Suzuki, J-I

    2010-05-01

    We investigated the growth of and soil exploration by Lolium perenne under a heterogeneous environment before its roots reached a nutrient-rich patch. Temporal changes in the distribution of inorganic nitrogen, i.e., NO(3)(-)-N and NH(4)(+)-N, in the heterogeneous environment during the experimental period were also examined. The results showed that roots randomly explored soil, irrespective of the patchy distribution of inorganic nitrogen and differences in the chemical composition of inorganic nitrogen distribution between heterogeneous and homogeneous environments. We have also elucidated the potential effects of patch duration and inorganic nitrogen distribution on soil exploration by roots and thus on plant growth.

  4. Utilización del suero de queso en polvo y la harina de soya, como fuentes de sólidos en la elaboración de yoghurt

    Directory of Open Access Journals (Sweden)

    Peña Aurora

    1988-06-01

    Full Text Available Con el objetivo de incrementar los sólidos no grasos en el yoghurt, se utilizaron materias primas no tradicionales, con ventajas nutritivas y económicas, como el suero de queso en polvo y la harina de soya semidesengrasada, en niveles independientes o en mezclas entre el 1 y 2%. El nivel del 2% de suero de queso en polvo, presentó características físico-químicas y organolépticas favorables, que lo acreditan como posible sustitutivo de la leche en polvo descremada, reduciendo en 6% el costo por litro de yoghurt.The purpose was to increase non fatty solids in the yoghurt, using non traditional raw materials, with nourishing and economic advantages as the dried cheese whey and semidefatted soybean flour, in mixtures of 1 and 2% independent levels. The level of replacement with 2% of dried cheese whey, showed favorable organoleptic and physical and chemical conditions that merit as a dried skim milk possible substitute, reducing 6% cost per litre of yoghurt.

  5. Antioxidant Effect of Pollen Grains and Soya Lecithin on Cadmium-induced Biochemical and Structural disorders in the Ovary of Female Rats during Estrus Cycle

    International Nuclear Information System (INIS)

    Mansour, S. Z.; Ramadan, F. L.

    2010-01-01

    This work aims at assessing the role of pollen grains with soya lecithin on cadmium-induced damage. Cadmium chloride was administered to female albino rats (0.5 mg/kg b. wt, i.p.) during 6 weeks. Pollen grains (54 mg/kg b.wt), and soya lecithin (18 mg/kg b.wt), were given, via gavages, 7 days before cadmium administration, and during cadmium treatment. The results demonstrate that cadmium exposure induces different distortions in ovarian tissues, fibrotic follicular cortex, appearance of atretic follicles, partial oocytes degeneration and significant decreases in the number of primordial, primary, secondary and antral follicles associated with significant increase in MDA levels , significant decreases in GSH content, GSH-Px, SOD and Cat activities. Significant increases in total saturated and monounsaturated fatty acids and significant decreases in polyunsaturated fatty acids levels were recorded. Significant decreases in plasma calcium, progesterone, estradiol and HDL-C and significant increases in triglycerides, total cholesterol, LDL-C levels were recorded. Administration of pollen grains with soya lecithin has significantly improved the antioxidant status and fatty acids levels associated with regeneration of ovarian tissues. Significant amelioration in saturated and unsaturated fatty acids, and hormones levels were also recorded. It is concluded that pollen grains with soya lecithin may protect the ovary during estrus cycle from cadmium-induced toxicity. Key words: pollen, soya lecithin, cadmium, oxidative stress, ovary, fatty acids, follicle numbers

  6. Identification of loci governing eight agronomic traits using a GBS-GWAS approach and validation by QTL mapping in soya bean.

    Science.gov (United States)

    Sonah, Humira; O'Donoughue, Louise; Cober, Elroy; Rajcan, Istvan; Belzile, François

    2015-02-01

    Soya bean is a major source of edible oil and protein for human consumption as well as animal feed. Understanding the genetic basis of different traits in soya bean will provide important insights for improving breeding strategies for this crop. A genome-wide association study (GWAS) was conducted to accelerate molecular breeding for the improvement of agronomic traits in soya bean. A genotyping-by-sequencing (GBS) approach was used to provide dense genome-wide marker coverage (>47,000 SNPs) for a panel of 304 short-season soya bean lines. A subset of 139 lines, representative of the diversity among these, was characterized phenotypically for eight traits under six environments (3 sites × 2 years). Marker coverage proved sufficient to ensure highly significant associations between the genes known to control simple traits (flower, hilum and pubescence colour) and flanking SNPs. Between one and eight genomic loci associated with more complex traits (maturity, plant height, seed weight, seed oil and protein) were also identified. Importantly, most of these GWAS loci were located within genomic regions identified by previously reported quantitative trait locus (QTL) for these traits. In some cases, the reported QTLs were also successfully validated by additional QTL mapping in a biparental population. This study demonstrates that integrating GBS and GWAS can be used as a powerful complementary approach to classical biparental mapping for dissecting complex traits in soya bean. © 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  7. Nutritional quality of fermented defatted soya and flaxseed flours and their effect on texture and sensory characteristics of wheat sourdough bread.

    Science.gov (United States)

    Bartkiene, Elena; Juodeikiene, Grazina; Vidmantiene, Daiva

    2012-09-01

    The use of soya and flaxseed flours fermented with Pediococcus acidilactici for wheat sourdough bread production was investigated. The protein digestibility, biogenic amine contents of soya and flaxseed sourdoughs, texture and sensory features of bread were studied. The fermentation with P. acidilactici significantly improved soya and flaxseed protein extraction and increased protein digestibility on an average by 13.5%. The concentrations of histamine (3.8 ± 2.3 and 4.0 ± 0.2 mg/kg), tyramine (4.6 ± 0.7 and 19.3 ± 1.8 mg/kg) and putrescine (66.4 ± 1.3 and 11.3 ± 3.0 mg/kg) do not present a health risk for consumers due to their relatively low levels in fermented plant products. The flaxseed sourdoughs influenced a 17.5% higher specific volume and a 4.6% lower crumb hardness of bread than those of soya sourdoughs, and did not disimprove sensory properties of bread. However, the fermented soya additives decreased acceptability of bread because of intensive taste and odour.

  8. Efectos de la suplementación con bloques multinutricionales a base de nopal fermentado sobre la ganancia de peso de ovinos en crecimiento Efectos de la suplementación con bloques multinutricionales a base de nopal fermentado sobre la ganancia de peso de ovinos en crecimiento

    OpenAIRE

    Isidro Guajardo Hernández; Ignacio Mejía Haro; José Luis Delgado Hernández; Mauricio Valencia Posadas; José Mejía Haro

    2012-01-01

    Este trabajo presenta una alternativa práctica del aprovechamiento del nopal mediante la elaboración de Bloques Multinutricionales (BM) y su utilización en la alimentación de corderos en crecimiento. Al nopal fresco picado se le adicionó sorgo, soya, urea, minerales y yogurt natural, se dejó fermentar por dos semanas a temperatura ambiente y se elaboraron los BM con un 25 % de nopal fermentado. Se utilizaron 20 corderos destetados con un peso vivo promedio de 17 Kg distribuidos en dos grupos ...

  9. Fitasa y enzimas fibrolíticas en dietas para cerdos con diferentes sustratos

    Directory of Open Access Journals (Sweden)

    Mario Alberto Ávalos Castro

    2011-01-01

    Full Text Available El objetivo del estudio fue evaluar la adición de fitasa (F y actividades de pectinasa, glucanasa y hemicelulasa (PGH en dietas sorgo-soya-canola, y xilanasa (X en dietas con grano seco de destilería con solubles (GSDS sobre la digestibilidad ileal y fecal de nutrientes (Exp 1 y el comportamiento productivo (Exp 2 en cerdos en crecimiento finalización. En el Exp 1 se formularon dos dietas basales (B1, sorgo-soya-canola y B2, sorgo-soya-canola + GSDS y los tratamientos fueron, 1 B1+F, 2 B1+F+PGH, 3 B2+F, 4 B2+F+PGH, 5 B2+F+X, y 6 B2+F+PGH+X. El Exp 2 incluyó cuatro tratamientos, 1 Testigo positivo (sorgosoya- canola con energía normal, 2 Igual que 1, pero bajo en energía, 3 Igual que 2 +F+PGH, 4 Igual que 3 +GSDS +X. En el Exp 1, la digestibilidad de la energía a nivel ileal se mejoró (P<0.05 con +F+PGH, +F+X y +F+PGH+X, y a nivel fecal se mejoró (P<0.05 con +F+X. La PGH liberó 122 kcal a nivel ileal y la X liberó 147 y 132 kcal de ED/kg de alimento a nivel ileal y fecal. En el Exp 2, la eficiencia alimenticia fue mayor (P<0.01 en el testigo positivo y el tratamiento bajo en energía +F+PGH, pero este último tuvo mayor (P<0.05 eficiencia energética. El valor energético de los GSDS se mejoró por la adición de F, PGH y X, pero la respuesta en crecimiento observada en el presente trabajo no fue consistente.

  10. High pressure inactivation of Pseudomonas in black truffle - comparison with Pseudomonas fluorescens in tryptone soya broth

    Science.gov (United States)

    Ballestra, Patricia; Verret, Catherine; Cruz, Christian; Largeteau, Alain; Demazeau, Gerard; El Moueffak, Abdelhamid

    2010-03-01

    Pseudomonas is one of the most common genera in black Perigord truffle. Its inactivation by high pressure (100-500 MPa/10 min) applied on truffles at sub-zero or low temperatures was studied and compared with those of Pseudomonas fluorescens in tryptone soya broth. Pressurization of truffles at 300 MPa/4 °C reduced the bacterial count of Pseudomonas by 5.3 log cycles. Higher pressures of 400 or 500 MPa, at 4 °C or 20 °C, allowed us to slightly increase the level of destruction to the value of ca. 6.5 log cycles but did not permit us to completely inactivate Pseudomonas. The results showed a residual charge of about 10 CFU/g. Pressure-shift freezing of truffles, which consists in applying a pressure of 200 MPa/-18 °C for 10 min and then quickly releasing this pressure to induce freezing, reduced the population of Pseudomonas by 3.3 log cycles. The level of inactivation was higher than those obtained with conventional freezing. Endogenous Pseudomonas in truffle was shown to be more resistant to high pressure treatments than P. fluorescens used for inoculation of broths.

  11. Green synthesis of soya bean sprouts-mediated superparamagnetic Fe3O4 nanoparticles

    International Nuclear Information System (INIS)

    Cai Yan; Shen Yuhua; Xie Anjian; Li Shikuo; Wang Xiufang

    2010-01-01

    Superparamagnetic Fe 3 O 4 nanoparticles were first synthesized via soya bean sprouts (SBS) templates under ambient temperature and normal atmosphere. The reaction process was simple, eco-friendly, and convenient to handle. The morphology and crystalline phase of the nanoparticles were determined from scanning electron microscopy (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), and X-ray diffraction (XRD) spectra. The effect of SBS template on the formation of Fe 3 O 4 nanoparticles was investigated using X-ray photoemission spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FT-IR). The results indicate that spherical Fe 3 O 4 nanoparticles with an average diameter of 8 nm simultaneously formed on the epidermal surface and the interior stem wall of SBS. The SBS are responsible for size and morphology control during the whole formation of Fe 3 O 4 nanoparticles. In addition, the superconducting quantum interference device (SQUID) results indicate the products are superparamagnetic at room temperature, with blocking temperature (T B ) of 150 K and saturation magnetization of 37.1 emu/g.

  12. Green synthesis of soya bean sprouts-mediated superparamagnetic Fe 3O 4 nanoparticles

    Science.gov (United States)

    Cai, Yan; Shen, Yuhua; Xie, Anjian; Li, Shikuo; Wang, Xiufang

    2010-10-01

    Superparamagnetic Fe 3O 4 nanoparticles were first synthesized via soya bean sprouts (SBS) templates under ambient temperature and normal atmosphere. The reaction process was simple, eco-friendly, and convenient to handle. The morphology and crystalline phase of the nanoparticles were determined from scanning electron microscopy (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), and X-ray diffraction (XRD) spectra. The effect of SBS template on the formation of Fe 3O 4 nanoparticles was investigated using X-ray photoemission spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FT-IR). The results indicate that spherical Fe 3O 4 nanoparticles with an average diameter of 8 nm simultaneously formed on the epidermal surface and the interior stem wall of SBS. The SBS are responsible for size and morphology control during the whole formation of Fe 3O 4 nanoparticles. In addition, the superconducting quantum interference device (SQUID) results indicate the products are superparamagnetic at room temperature, with blocking temperature ( TB) of 150 K and saturation magnetization of 37.1 emu/g.

  13. Pancreatic growth and cell turnover in the rat fed raw soya flour

    International Nuclear Information System (INIS)

    Oates, P.S.; Morgan, R.G.

    1982-01-01

    Growth and differentiation of the pancreatic acinar cell was studied in rats fed raw soya flour (RSF) for up to a year. A second group of rats were fed a control diet. After 1 week of RSF feeding there was a 200% increase in tissue RNA and weight, indicating initial hypertrophy, which was maintained for the 1-year study period. By the second week and over the remainder of the period studied there was also a marked increase in total DNA, suggesting hyperplasia. Cell turnover, as measured by the rate of incorporation of 3H-thymidine into pancreatic DNA, was significantly higher in RSF-fed animals only from the second to fourth weeks; it then returned to control values. Autoradiography showed an 18-fold increase in duct cell labeling at the end of the first week and an 11-fold increase by the end of the second week. Acinar cell labeling doubled from the second to the twelfth week. These studies confirm previous reports that RSF produces pancreatic hypertrophy and hyperplasia. They furthermore show that there is initially marked stimulation of DNA synthesis in the duct cell compartment. The results suggest that cells with the morphologic characteristics of duct cells may be the precursors of acinar cells in hyperplastic pancreatic tissue

  14. Soya phospholipid complex of mangiferin enhances its hepatoprotectivity by improving its bioavailability and pharmacokinetics.

    Science.gov (United States)

    Bhattacharyya, Sauvik; Ahmmed, Sk Milan; Saha, Bishnu Pada; Mukherjee, Pulok K

    2014-05-01

    Mangiferin is a xanthonoid present in Mangifera indica. It has been reported for a variety of pharmacological activities, including hepatoprotection. However, the major disadvantage of mangiferin is its reduced biological activity due to poor absorption, low bioavailability and rapid elimination from the body after administration. The aim of this study was to prepare a phospholipid complex of mangiferin to overcome these limitations and to investigate the impact of the complex on hepatoprotective activity and bioavailability. The results showed that the complex has an enhanced hepatoprotective and in vivo antioxidant activity as compared to pure mangiferin at the same dose level (30 and 60 mg kg⁻¹). The complex restored the levels of serum hepatic marker enzymes and liver antioxidant enzymes with respect to carbon tetrachloride-treated animals. The complex also increased the bioavailability of mangiferin in rat serum by 9.75-fold compared to pure mangiferin at the same dose level and enhanced the elimination half-life (t(1/2 el)) from 1.71 ± 0.12 h⁻¹ to 3.52 ± 0.27 h⁻¹. The results suggested that the complexation of mangiferin with soya phospholipid enhanced the hepatoprotection and in vivo antioxidant activity, which may be due to the improved bioavailability and pharmacokinetics of mangiferin in rat serum. © 2013 Society of Chemical Industry.

  15. Formation of thermally induced aggregates of the soya globulin beta-conglycinin.

    Science.gov (United States)

    Mills, E N; Huang, L; Noel, T R; Gunning, A P; Morris, V J

    2001-06-11

    The effect of ionic strength (I) on the formation of thermally induced aggregates by the 7S globular storage protein of soya, beta-conglycinin, has been studied using atomic force microscopy. Aggregates were only apparent when I> or =0.1, and had a fibrous appearance, with a height (diameter) of 8-11 nm. At high ionic strength (I=1.0) the aggregates appeared to associate into clumps. When aggregate formation was studied at I=0.2, it was clear that aggregation only began at temperatures above the main thermal transition for the protein at 75 degrees C, as determined by differential scanning calorimetry. This coincided with a small change in secondary structure, as indicated by circular dichroism spectroscopy, suggesting that a degree of unfolding was necessary for aggregation to proceed. Despite prolonged heating the size of the aggregates did not increase indefinitely, suggesting that certain beta-conglycinin isoforms were able to act as chain terminators. At higher protein concentrations (1% w/v) the linear aggregates appeared to form large macroaggregates, which may be the precursors of protein gel formation. The ability of beta-conglycinin to form such distinctive aggregates is discussed in relation to the presence of acidic inserts in certain of the beta-conglycinin subunits, which may play an important role in limiting aggregate length.

  16. Rheology and microstructure of gluten and soya-based o/w emulsions

    Energy Technology Data Exchange (ETDEWEB)

    Bengoechea, Carlos; Cordobes, Felipe; Guerrero, Antonio [Universidad de Sevilla, Departamento de Ingenieria Quimica, Sevilla (Spain)

    2006-10-15

    Highly concentrated oil-in-water (o/w) emulsion stabilised by means of gluten and soya protein isolate (SPI) at low pH have been characterized by means of linear dynamic viscoelasticity and droplet size distribution analysis (DSD). The microstructure of these emulsions has been characterized at a colloidal level by using confocal laser scanning microscopy (CLSM) and light microscopy (LM). These emulsions always exhibited a behaviour characteristic of highly flocculated emulsions with a mechanical spectrum showing a well-developed plateau region. DSD results generally showed log normal bimodal profiles. Microstructure images revealed occurrence of a close packing of droplets with a broad distribution of sizes participating in the formation of a three dimensional flocculated network. The Mason model of elasticity of compressed emulsions has been used to correlate viscoelastic and microstructural parameters giving adequate fitting but underestimating the elastic properties obtained for the highest concentration of gluten. These deviations may be explained in terms of an enhancement of the elastic network formed in the aqueous phase in which the glutenin fraction must play an important role. (orig.)

  17. PHYTOTHERAPEUTIC EFFECT OF AVOCADOS AND SOYA AS DRUG ON HEART OF RATS EXPOSED TO GAMMA RADIATION

    International Nuclear Information System (INIS)

    OMRAN, M.F.; IBRAHIM, N.K.; ABU-ZIED, N.M.

    2008-01-01

    This study was planed to determine the role of single oral dose of avocados and soya in irradiated rats exposed to gamma radiation. the experimental animals were randomly divided into four groups; 12 rats for each. Group 1: kept as control. Group 2: rats received piascledine for 14 consecutive days. Group 3: rats submitted to whole body gamma rays (4 Gy). Group 4: rats received the same piascledine 14 days post-exposure to 4 Gy gamma radiation. The animals were weighed then dissected after one and fourteen days post-administration and the cardiac tissue and plasma were stored at 12 oC till used for biochemical analysis and kept in ice. The following parameters were determined: plasma total cholesterol, triacylglycerol, high density lipoprotein-cholesterol, low density lipoprotein-cholesterol, LDH, phospholipids, ALT, CPK and TBARS. In cardiac tissue, determinations of total cholesterol, triacylglycerol, phospholipids and TBARS were conducted.It can be concluded that administration of avocados and soy as natural drug (piascledine) post-irradiation in rats is a favorable modificator against the impaired physiological processes in animal body due to gamma irradiation

  18. STUDY ON PHYTO-EXTRACTION BALANCE OF ZN, CD AND PB FROM MINE-WASTE POLLUTED SOILS BY USING FESTUCA ARUNDINACEA AND LOLIUM PERENNE SPECIES

    Directory of Open Access Journals (Sweden)

    B. LIXANDRU

    2009-05-01

    Full Text Available Through the cultivation of tall fescue (Festuca arundinacea and of perennial ryegrass for two years on a chernozem type of soil, in the Banat's plain area we investigated the phyto-extraction potential of Zn, Cd and Pb. In the experimental plot it has been incorporated a quantity of 20 kg of mine-waste per square meter, in a mass ratio of 1:2,5. The mine-waste polluting "contribution" was of 1209 mg Zn / kg d.s., 4.70 mg Cd / kg d.s. and 188.2 mg Pb / kg d.s. The metals content in the soil was determined at the two moments of biomass harvesting, and through balance calculations we could establish the phyto-extraction efficiency of the two foragegrasses species. The obtained results indicate that Festuca arundinacea has an average phyto-extraction yield of 50% for Zn and Cd in the soil; in the case of an ionic excess of 3,5 to 4 times, the phyto-extraction efficiency is reduced, more obvious in the case of Pb (lead ions. The species Lolium perenne registers a yield of almost 92% in the process of phyto-extraction of Zn. The yield values for Cd si Pb are lower, but comparable with the control plot. Unlike Festuca arundinacea, the Lollium perenne species tolerates better the Cd and Pb ionic excess.

  19. The photosynthetic acclimation response of Lolium perenne to four years growth in a free-air CO{sub 2} enrichment (FACE) facility

    Energy Technology Data Exchange (ETDEWEB)

    Creasey, R. [Univ. of Essex (United Kingdom)

    1996-11-01

    In this study, the photosynthetic responses of field grown Lolium perenne to ambient (354 {mu}mol mol{sup -1}) and elevated (600 {mu}mol mol{sup -1}) C{sub a} were measured. The experiment utilized the FACE facility at Eschikon, Switzerland; here the L. Perenne swards had been grown at two nitrogen treatments, with six cuts per year, for 4 years. The study revealed a significant decrease in Rubisco activity (Vcmax) in the low nitrogen FACE plots; this is consistent with the theories of source-sink imbalance resulting in feedback inhibition and down-regulation. Such negative acclimation was not wholly supported by diurnal investigations which revealed an average stimulation of 53.38% and 52.78% in the low and high nitrogen, respectively. However, light response curves and AI investigations also suggested down-regulation, especially in the low nitrogen. SI is expected to decrease in response to elevated C{sub a}, if any change is seen. This was indeed observed in the high nitrogen plots but for the low nitrogen a significant increase was found. Conclusions drawn from this project center around the implications of negative acclimation to future crop productivity. For instance, inter-specific differences in response to elevated C{sub a} may result in ecosystem changes and new management techniques may be necessary. However, real predictions cannot be made from leaf level studies alone as these may not represent the overall changes at the whole plant level.

  20. Efficiency of Opuntia ficus in the phytoremediation of a soil contaminated with used motor oil and lead, compared to that of Lolium perenne and Aloe barbadensis.

    Science.gov (United States)

    Escobar-Alvarado, Luisa F; Vaca-Mier, Mabel; López-Callejas, Raymundo; Rojas-Valencia, Ma Neftalí

    2018-01-28

    Industrial pollutants such as heavy metals and hydrocarbons in soils represent a serious concern due to their persistence and negative effects on the environment, affecting cellular processes in living organisms and even causing mutations and cancer. The main objectives of this work were to evaluate the efficiency of Opuntia ficus in the phytoremediation of a soil polluted with used motor oil. Two other species, one with different and one with similar characteristics, relatively, were used for comparison purposes: Lolium perenne and Aloe barbadensis. The effect of the plants on lead solubility and bioaccumulation, the biomass production of each specie and the microbial counts and bacterial identification for each experiment was studied. Total petroleum hydrocarbons (TPH) were measured every 5 weeks throughout the 20-week phytoremediation experiment. At the end of the experiment soluble Pb, Pb extracted by the plant species, microbiological counts, total biomass and bacterial species in soil were analyzed. Even though Lolium perenne showed the highest TPH removal (47%), Opuntia ficus produced the highest biomass and similar removal (46%). Since Opuntia ficus requires low amounts of water and grows fast, it would be a suitable option in the remediation of soils polluted with hydrocarbons and/or heavy metals.

  1. Sustitución parcial del alimento balanceado por ensilaje de soya y su efecto en la producción y calidad de la leche de vaca, en el trópico húmedo de Costa Rica

    Directory of Open Access Journals (Sweden)

    Carlos Tobía

    2004-01-01

    Full Text Available Se evaluó el efecto de la sustitución parcial (0, 13,6 y 27,2% de un alimento balanceado (AB por el ensilaje de soya, con melaza de caña al 8% e inoculado con Lactobacillus brevis 3 (ES, en las dietas de vacas Holstein sobre la producción y calidad de la leche. Los 3 tratamientos se asignaron a 12 vacas de primera lactancia, con un peso promedio de 550 kg y con una producción promedio diaria de 18±3 kg de leche, distribuidas en un diseño experimental “cross over”. La sustitución del 13,6% del AB por ES no afectó el consumo de materia seca (MS ni la producción de leche, pero sí redujo en un 7% los costos de alimentación. La sustitución del 27,2% del AB por ES redujo el consumo de MS (P² 0,05 y la producción promedio de leche en 1,6 kg vaca-1 día-1 (P² 0,05. La introducción del ES en la dieta no afectó el porcentaje de grasa ni el contenido de sólidos totales, pero sí redujo (P² 0,05 el porcentaje de proteína y lactosa de la leche. Estos resultados aportan evidencia de que la introducción del ES, como sustituto parcial del AB en la dieta, es una alternativa viable para reducir los costos de producción de leche en el trópico.

  2. Dietary soya protein improves intra-myocardial lipid deposition and altered glucose metabolism in a hypertensive, dyslipidaemic, insulin-resistant rat model.

    Science.gov (United States)

    Oliva, María E; Creus, Agustina; Ferreira, María R; Chicco, Adriana; Lombardo, Yolanda B

    2018-01-01

    This study investigates the effects of replacing dietary casein by soya protein on the underlying mechanisms involved in the impaired metabolic fate of glucose and lipid metabolisms in the heart of dyslipidaemic rats chronically fed (8 months) a sucrose-rich (62·5 %) diet (SRD). To test this hypothesis, Wistar rats were fed an SRD for 4 months. From months 4 to 8, half the animals continued with the SRD and the other half were fed an SRD in which casein was substituted by soya. The control group received a diet with maize starch as the carbohydrate source. Compared with the SRD-fed group, the following results were obtained. First, soya protein significantly (Psoya protein significantly increased (Psoya protein upon the altered pathways of glucose and lipid metabolism in the heart muscle of this rat model.

  3. Aplicación de diferentes fuentes de azufre en soya (Glycine max (L. Merrill en tres ordenes de suelos del Valle del río Cauca, Colombia, en condiciones de invernadero

    Directory of Open Access Journals (Sweden)

    Gómez L. Jairo A.

    1989-06-01

    Full Text Available Se utilizaron cuatro fuentes portadoras de S (sulfato de amonio, yeso, ácido sulfúrico y azufre micronizado en dosis de 20 y 40 kg de S/ha, aplicado en pre y al momento de la siembra , en tres órdenes de suelos (Vertisol, Inceptisol y Entisol del Valle geográfico del río Cauca, con la variedad ICA Tunía, como cultivo indicador. La unidad experimental estuvo constituida por bolsas de polietileno con dos kilos de suelo seco al aire, utilizando el horizonte Ap. El diseño experimental fue completamente al azar, el diseño de tratamientos fueron dos factoriales entrelazados con dos tratamientos adicionales, testigo absoluto (To, testigo comercial (Te. Este llevó fertilización basal con N, P, K, y doce tratamientos con fertilización basal más S (N, P, K +S. Al período de floración se cuantificó peso de materia seca, peso de nódulos, % S, % N Y N/S, a final de cosecha se determinó en el grano de soya % S, % N, N/ S, número de vainas, de granos, peso seco de grano y % de aceite. Los tres suelos respondieron a la fertilización de S. La fuente que presentó diferencias significativas con mayor incremento en producción de grano de soya, fue el azufre micronizado, en la dosis de 20 kg S/ha aplicado con anterioridad a la siembra.In order to fertilize, four sources of sulphur, ammonicum sulfate, gyppum, sulfuric acid and micronized sulphur, were used. Sulphur dose of 20 and 40 kg per hectare were applied before sowing and at the seeding moment, on three soils orders (Vertisol, Inceptisol and Entisol of the geographic Valley of the Cauca river, with the soybeam variety ICA Tunía. The experimental units were made of polyethilen bags with two kilograms of air dry soil, using the Ap. horizon. The experimental design was completyly ramdom. The treatment designs were two factorial s interlaced with two additional treatments, absolute indicator (To commercial indicator (Te. This carried basal fertilization with N, P, K and twelve treatments with

  4. Assesing Bemisia tabaci (Genn. biotype B resistance in soybean genotypes: Antixenosis and antibiosis Evaluación de la resistencia de genotipos de soya a Bemisia tabaci (Genn. biotipo B: Antixenosis y antibiosis

    Directory of Open Access Journals (Sweden)

    José Paulo Gonçalves Franco da Silva

    2012-12-01

    Full Text Available Since it was first reported in Brazil in the 1990s, the B biotype of silverleaf whitefly (Bemisia tabaci Genn., Hemiptera: Aleyrodidae has been recognized as an important pest in soybeans (Glycine max L., reducing the productivity of this legume species in some areas of the country. As an alternative to chemical control, the use of resistant genotypes represents an important tool for integrated pest management (IPM. This study evaluated the performance of 10 soybean genotypes prior to whitefly infestation, by testing attractiveness and preference for oviposition in the greenhouse and antibiosis in the laboratory. In a multiple-choice test, 'IAC-17' was the least attractive to insects. In a no-choice test, 'IAC-17' was the least attractive for egg deposition, indicating the occurrence of non-preference for oviposition on this genotype. Trichome density was positively correlated with the oviposition site and may be associated with the resistance of 'IAC-17' to infestation. The genotypes 'IAC-PL1', 'IAC-19', 'Conquista', 'IAC-24' and 'IAC-17' extended the insect's life cycle, indicating the occurrence of a small degree of antibiosis and/or non-preference for feeding.Desde que se registró por primera vez en Brasil en la década de 1990, el biotipo B de la mosca-blanca (Bemisia tabaci Genn., Hemiptera: Aleyrodidae, se reconoce como una importante plaga de la soya (Glycine max L. y es lo que reduce la productividad de estas especies de leguminosas en algunas zonas del pais. Como una alternativa al control químico, el uso de genotipos resistentes representa una herramienta importante para la gestión integrada de plagas (MIP. Este trabajo evaluó el comportamiento de 10 genotipos de soya frente al ataque de la mosca-blanca, por medio de ensayos de atractividad y preferencia para ovipostura en invernaderos y antibiosis en laboratorio. En una prueba de elección multiple, 'IAC-17' fue el menos atractivo para los insectos. En una prueba sin elecci

  5. Efecto del riego y profundidad de compactación en la producción de soya variedad valluna-5

    Directory of Open Access Journals (Sweden)

    Gallardo B. Carlos A.

    1992-12-01

    Full Text Available

    El experimento se realizó durante el segundo semestre de 1992 en el Centro de Investigaciones del ICA en Palmira, con el objetivo de evaluar el efecto de diferentes profundidades de compactación y niveles de  humedad sobre la producción de soya variedad Valluna-5. En un suelo franco sin deficiencias, ni toxicidad por elementos, se estableció el experimento en un arreglo en franjas divididas, con cuatro repeticiones: las parcelas principales fueron los tres niveles de riego y las subparcelas las profundidades de compactación (5, 15 y 30 cm y testigo. El nivel de humedad afectó el rendimiento, peso de 100 semillas y porcentaje de germinación.; mientras que las profundidades de compactación restringieron más la profundidad de raíces. Los tratamientos no afectaron la altura de planta, área foliar, calidad de semilla, porcentaje de grasas y proteínas y toma de elementos (N, P, K, Ca, Mg, Mn, Zn, Cu, Fe y B.

     At Instituto Colombiano Agropecuario (ICA in Palmira, an experiment was carried out during the second semester of 1992 to evaluate the effect of different compaction depths and moisture levels on the soybean Valluna - 5 variety. On a loam soil without deficiencies nor toxicity of elements, the experiment was stablished under a split plot arrangement with four repetitions where the principal plots were the three irrigation levels and the subplots were the compaction depths (5, 15, 30 cm and  control. The moisture level was more influential than the compaction depth respect to the yield, weight of 100 seeds and germination percentage, while the compaction depth restricted more the root depth. Height of plants, foliar area, seed quality, oil and protein percentages and uptake of elements (N, P, K, Ca, MG, Mn, Zn, Cu, Fe and B were not affected by the treatments.

  6. Inactivation of Staphylococcus aureus in Oat and Soya Drinks by Enterocin AS-48 in Combination with Other Antimicrobials.

    Science.gov (United States)

    Burgos, María José Grande; Aguayo, M Carmen López; Pulido, Rubén Pérez; Gálvez, Antonio; López, Rosario Lucas

    2015-09-01

    The presence of toxicogenic Staphylococcus aureus in foods and the dissemination of methicillin-resistant S. aureus (MRSA) in the food chain are matters of concern. In the present study, the circular bacteriocin enterocin AS-48, applied singly or in combination with phenolic compounds (carvacrol, eugenol, geraniol, and citral) or with 2-nitro-1-propanol (2NPOH), was investigated in the control of a cocktail made from 1 methicillin-sensitive and 1 MRSA strains inoculated on commercial oat and soya drinks. Enterocin AS-48 exhibited low bactericidal activity against staphylococci in the drinks investigated when applied singly. The combinations of sub-inhibitory concentrations of enterocin AS-48 (25 μg/mL) and phenolic compounds or 2NPOH caused complete inactivation of staphylococci in the drinks within 24 h of incubation at 22 °C. When tested in oat and soya drinks stored for 7 d at 10 °C, enterocin AS-48 (25 μg/mL) in combination with 2NPOH (5.5 mM) reduced viable counts rapidly in the case of oat drink (4.2 log cycles after 12 h) or slowly in soya drink (3.8 log cycles after 3 d). The same combined treatment applied on drinks stored at 22 °C achieved a fast inactivation of staphylococci within 12 to 24 h in both drinks, and no viable staphylococci were detected for up to 7 d of storage. Results from the study highlight the potential of enterocin AS-48 in combination with 2NPOH for inactivation of staphylococci. © 2015 Institute of Food Technologists®

  7. INFLUENCIA DE LA CONCENTRACIÓN DE MELAZA Y EXTRACTO ACUOSO DE SOYA SOBRE LA VELOCIDAD ESPECÍFICA DE CRECIMIENTO DE Bradyrhizobium elkanii ICA 8001

    OpenAIRE

    Gretel Gómez; María C. Nápoles; R. Núñez; J. Martínez

    2008-01-01

    La cepa ICA 8001 de Bradyrhizobium elkanii es ampliamente usada en Cuba para la producción de inoculantes para soya. Uno de los medios de cultivos diseñados para la multiplicación de este microorganismo contiene melaza y extracto acuoso de soya, elementos de variable composición y origen, que aportan cantidades notables de sustratos carbonados. Por esta causa, se determinó la concentración de carbohidratos totales solubles y carbohidratos reductores, así como los contenidos de N, P, K, Ca, Mg...

  8. Efecto de la morfología de los embriones somáticos en la regeneración de plantas de soya (Glycine max L. Merrill)

    OpenAIRE

    Pérez-Pérez, Jorge L.; García-Rodríguez, Lourdes; Veitía-Rodríguez, Novisel; Bermúdes-Caraballoso, Idalmis; Collado-López, Raúl; Torres-Rodríguez, Damaris

    2017-01-01

    Los protocolos establecidos para la regeneración de plantas vía embriogénesis somática en soya (Glycine max L. Merrill), son específicos para un número reducido de cultivares, lo que requiere de nuevos estudios que permitan disponer de protocolos más eficientes y reproducibles en un mayor número de genotipos. Este trabajo tuvo como objetivo determinar el efecto del tipo de morfología de los embriones somáticos en la germinación y conversión a planta en soya cultivar ʻIncasoy-27ʼ. Se emplearon...

  9. Polish reference material: soya bean flour (INCT-SBF-4) for inorganic trace analysis - preparation and certification

    International Nuclear Information System (INIS)

    Polkowska-Motrenko, H.; Dybczynski, R.; Chajduk, E.; Danko, B.; Kulisa, K.; Samczynski, Z.; Sypula, M.; Szopa, Z.

    2006-01-01

    Preparation, examination and certification of the new matrix reference material of biological origin: Soya Bean Flour (INCT-SBF-4) is described. The material was prepared from soya bean grown in India, not genetically modified. After milling, the material was sieved through the 150 mm nylon sieves and stored in a polyethylene (PE) bag. Approximately 50 kg of sieved soya bean flour was collected. Examination by optical microscopy revealed that Martin's diameter of over 90% of particles was below 50 mm. The whole lot of soya bean flour was then homogenized by mixing for 20 hours in a 110 dm 3 PE drum rotated in three directions. Preliminary homogeneity testing by X-ray fluorescence (XRF) method and final checking of homogeneity by neutron activation analysis (NAA) after distribution of the material into containers revealed, that it is sufficiently homogeneous at least for a sample size ≥ 100 mg. In order to assure the long-term stability, all containers with INCT-SBF-4 were sterilized by electron beam radiation. Long-term stability was checked by analyzing concentrations of selected elements in the material stored in the air-conditioned room at 20 o C. Short-term stability was examined by the determination of concentrations of the selected elements in the bottle stored in the CO 2 incubator at 37 o C. The material was certified on the basis of a worldwide interlaboratory comparison, in which 92 laboratories from 19 countries participated providing 1107 laboratory averages (4873 individual determinations) for 58 elements. A method of data evaluation leading to assignment of certified values was the same as that used previously in the Laboratory of the Department of Analytical Chemistry, Institute of Nuclear Chemistry and Technology. The results for a few elements were obtained by definitive methods developed in the Laboratory and used to support the certification process. Analytical uncertainties and stability uncertainties were quantified to arrive at combined

  10. Diseño y fabricación de embutidos escaldados sustituyendo grasa porcina por aceite de soya

    OpenAIRE

    Vargas Vallejos, Calixto Evaristo

    2017-01-01

    Incorpora aceite de soya en sustitución de la grasa porcina en la formulación de dos tipos de embutidos: jamonada y hot dog. La metodología de la elaboración, proceso tecnológico y evaluación comprendió: el desarrollo de formulaciones estándares convencionales aceptadas por el IFT (Instituto de Tecnólogos de Alimentos) evaluaciones físicoquímicas mediante la caracterización química, incluyendo perfil de ácidos grasos (usando métodos oficiales de la AOAC), pruebas de aceptabilidad empleando pr...

  11. New Triterpenoid Saponins from Green Vegetable Soya Beans and Their Anti-Inflammatory Activities.

    Science.gov (United States)

    Lan, Xiuhua; Deng, Kejun; Zhao, Jianping; Chen, Yiyi; Xin, Xuhui; Liu, Yanli; Khan, Ikhlas A; Yang, Shilin; Wang, Taoyun; Xu, Qiongming

    2017-12-20

    Ten compounds were isolated and identified from green vegetable soya beans, of which five are new triterpenoid saponins (1-5) and five are known compounds (6-10). The chemical structures of the five triterpenoid saponins (1-5) were elucidated to be 3β,24-dihydroxy-22β,30-epoxy-30-oxoolean-12-en 3-O-α-l-rhamnopyranosyl-(1 → 2)-β-d-xylopyranosyl-(1 → 2)-β-d-glucuronopyranoside, 1; 3β,24-dihydroxy-22β,30-epoxy-30-oxoolean-12-en 3-O-α-l-rhamnopyranosyl-(1 → 2)-β-d-(3″-O-formyl)-galactopyranosyl-(1 → 2)-β-d-glucuronopyranoside, 2; 22-keto-3β,24-dihydroxy oleanane-12-ene 3-O-α-l-rhamnopyranosyl-(1 → 2)-β-d-(3″-O-formyl)-galactopyranosyl-(1 → 2)-β-d-glucuronopyranoside, 3; 3β,22β,24-trihydroxy oxyolean-18(19)-ene-29-acid 3-O-α-l-rhamnopyranosyl-(1 → 2)-β-d-galactopyranosyl-(1 → 2)-β-d-glucuronopyranoside, 4; and punicanolic acid 3-O-α-l-rhamnopyranosyl-(1 → 2)-β-d-galactopyranosyl-(1 → 2)-β-d-glucuronopyranoside, 5 from the spectroscopic data (IR, GTC/FID, HR-ESI-MS, and 1D and 2D NMR). The nitric oxide release inhibitions of compounds 1-10 in LPS-stimulated RAW264.7 cells were evaluated, and the data suggested that compounds 1, 2, and 5 might possess moderate anti-inflammatory activities, with IC 50 values of 18.8, 16.1, and 13.2 μM, respectively.

  12. Characteristics of the cold-water belt formed off Soya Warm Current

    Science.gov (United States)

    Ishizu, Miho; Kitade, Yujiro; Matsuyama, Masaji

    2008-12-01

    We examined the data obtained by acoustic Doppler current profiler, conductivity-temperature-depth profiler, and expendable bathythermograph observations, which were collected in the summers of 2000, 2001, and 2002, to clarify the characteristics of the cold-water belt (CWB), i.e., lower-temperature water than the surrounding water extending from the southwest coast of Sakhalin along the offshore side of Soya Warm Current (SWC) and to confirm one of the formation mechanisms of the CWB as suggested by our previous study, i.e., the upwelling due to the convergence of bottom Ekman transport off the SWC region. The CWB was observed at about 30 km off the coast, having a thickness of 14 m and a minimum temperature of 12°C at the sea surface. The CWB does not have the specific water mass, but is constituted of three representative water types off the northeast coast of Hokkaido in summer, i.e., SWC water, Fresh Surface Okhotsk Sea Water, and Okhotsk Sea Intermediate Water. In a comparison of the horizontal distributions of current and temperature, the CWB region is found to be advected to the southeast at an average of 40 ± 29% of the maximum current velocity of the SWC. The pumping speed due to the convergence of the bottom Ekman transport is estimated as (1.5-3.0) × 10-4 m s-1. We examined the mixing ratio of the CWB, and the results implied that the water mass of the CWB is advected southeastward and mixes with a water mass upwelling in a different region off SWC.

  13. Enhancement of absorption and hepatoprotective potential through soya-phosphatidylcholine-andrographolide vesicular system.

    Science.gov (United States)

    Jain, Pushpendra Kumar; Khurana, Navneet; Pounikar, Yogesh; Gajbhiye, Asmita; Kharya, Murli Dhar

    2013-06-01

    Andrographis paniculata is a medicinal herb used extensively for various ailments and contains therapeutically active phytoconstituent, andrographolide (AN). Although hepatoprotective activity of AN is established, but their bioavailability is restricted due to its rapid clearance. The aim of this study, therefore, was to formulate AN herbosomes (ANH) through complexation with naturally occurring soya-phosphatidylcholine (SPC), in order to enhance absorption. Prepared andrographolide-soy phosphatidylcholine (AN-SPC) complex prepared was subjected for characterisation of complex and formation of vesicular system known as ANH using rotary evaporation techniques. This complex was subjected to in vitro study using everted small intestine sac technique which showed significantly increased absorption of AN from the ANH as compared to the plain AN. The hepatoprotective potential of ANH and plain AN was evaluated using carbon tetrachloride inducing hepatotoxicity rat model and compared, in which ANH equivalent to 50 mg/kg of plain AN significantly restore serum glutamate oxalacetate transaminase (112.4 ± 9.67 for AN whereas 90.2 ± 4.23 for ANH) and serum glutamate pyruvate transaminase (109.3 ± 7.89 for AN whereas 90.6 ± 4.34 for ANH) level as compared to control group. The ANH showed significantly better absorption than plain AN and this effect of ANH was also comparable to the standard drug (Silymarin). The findings of present study reveal that ANH has better bioavailability as shown by in vitro absorption study and hence improved hepatoprotection as compared to plain AN at equivalent dose.

  14. Report on limnological, biological and ecological observations of lakes on the Soya Coast, East Antarctica

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    Sakae Kudoh

    2008-11-01

    Full Text Available Observations on the limnological properties, samplings of waters and bottom assemblages for biological and ecological studies, and some field experimental studies at several lakes in Soya Coast ice-free areas, were carried out during the austral summer season in the 49th Japanese Antarctic Research Expedition (JARE, 2007-2008. These studies were planned as one of the research projects named, "Studies on the changes of polar environments and ecosystems (P-3" and the monitoring studies named "Monitoring for ecosystems (M-4" during the 7th term of the Japanese Antarctic Research Expedition Plans. Field studies were done from 22 December 2007 to 13 February 2008, while our Ice Breaker Shirase stayed at/near off Syowa Station. To clarify the relationships among seasonal changes of environmental factors and biological responses, frequent field observations were performed at Naga Ike, one of the freshwater lakes in the Skarvsnes ice-free area. General limnological and biological samplings at the other lakes in the area (14 lakes near the Kizahasi Beach field base camp were also done during the term. Observations and samplings distant from the base camp, four lakes in eastern Skarvsnes, a lake in Skallen, and three lakes in Langhovde, were also done using a helicopter for transportation. From Namazu Ike (temporary name in eastern Skarvsnes, submersible video cameras were retrieved and so-called `algal crest', benthic moss-algal assemblages, were sampled by scuba diving. Benthic copepods were sampled quantitatively from Nurume Ike in Langhovde. From Hyoga Ike (temporary name, a snow-dammed glacial lake which lost its water by recent breakage (during the JARE-46 wintering period, thin bio-film samples were collected from the present lake shore formerly part of the lake bed.

  15. Specific protein supplementation using soya, casein or whey differentially affects regional gut growth and luminal growth factor bioactivity in rats; implications for the treatment of gut injury and stimulating repair.

    Science.gov (United States)

    Marchbank, Tania; Mandir, Nikki; Calnan, Denis; Goodlad, Robert A; Podas, Theo; Playford, Raymond J

    2018-01-24

    Modulation of regional growth within specific segments of the bowel may have clinical value for several gastrointestinal conditions. We therefore examined the effects of different dietary protein sources on regional gut growth and luminal growth factor bioactivity as potential therapies. Rats were fed for 14 days on isonitrogenous and isocaloric diets comprising elemental diet (ED) alone (which is known to cause gut atrophy), ED supplemented with casein or whey or a soya protein-rich feed. Effects on regional gut growth and intraluminal growth factor activity were then determined. Despite calorie intake being similar in all groups, soya rich feed caused 20% extra total body weight gain. Stomach weight was highest on soya and casein diets. Soya enhanced diet caused greatest increase in small intestinal weight and preserved luminal growth factor activity at levels sufficient to increase proliferation in vitro. Regional small intestinal proliferation was highest in proximal segment in ED fed animals whereas distal small intestine proliferation was greater in soya fed animals. Colonic weight and proliferation throughout the colon was higher in animals receiving soya or whey supplemented feeds. We conclude that specific protein supplementation with either soya, casein or whey may be beneficial to rest or increase growth in different regions of the bowel through mechanisms that include differentially affecting luminal growth factor bioactivity. These results have implications for targeting specific regions of the bowel for conditions such as Crohn's disease and chemotherapy.

  16. Soya bean Gα proteins with distinct biochemical properties exhibit differential ability to complement Saccharomyces cerevisiae gpa1 mutant.

    Science.gov (United States)

    Roy Choudhury, Swarup; Wang, Yuqi; Pandey, Sona

    2014-07-01

    Signalling pathways mediated by heterotrimeric G-proteins are common to all eukaryotes. Plants have a limited number of each of the G-protein subunits, with the most elaborate G-protein network discovered so far in soya bean (Glycine max, also known as soybean) which has four Gα, four Gβ and ten Gγ proteins. Biochemical characterization of Gα proteins from plants suggests significant variation in their properties compared with the well-characterized non-plant proteins. Furthermore, the four soya bean Gα (GmGα) proteins exhibit distinct biochemical activities among themselves, but the extent to which such biochemical differences contribute to their in vivo function is also not known. We used the yeast gpa1 mutant which displays constitutive signalling and growth arrest in the pheromone-response pathway as an in vivo model to evaluate the effect of distinct biochemical activities of GmGα proteins. We showed that specific GmGα proteins can be activated during pheromone-dependent receptor-mediated signalling in yeast and they display different strengths towards complementation of yeast gpa1 phenotypes. We also identified amino acids that are responsible for differential complementation abilities of specific Gα proteins. These data establish that specific plant Gα proteins are functional in the receptor-mediated pheromone-response pathway in yeast and that the subtle biochemical differences in their activity are physiologically relevant.

  17. Egg shell and yolk quality characteristics of layers fed with sugarcane press residue in soya and fish based diets

    Directory of Open Access Journals (Sweden)

    N. Suma

    2015-02-01

    Full Text Available Aim: Sugarcane press residue (SPR, a by-product of sugarcane industry, which is rich in inorganic salts was assessed at different levels in both soya based and fish based diets of layers for egg shell and yolk quality characteristics. Materials and Methods: SPR was incorporated in 32-week-old white leghorn layer diets at 0%, 5%, 10% and 15% either in the soya based or fish based diets to form T1 to T8 diets, respectively. Each diet was offered to five replicates of four laying hens each constituting a total of one sixty birds kept for 84 days under colony cages. Results: Mean egg shell thickness obtained from eggs of experimental hens measured was 0.342, 0.329, 0.320, 0.322, 0.319, 0.332, 0.328 and 0.336 mm in T1 through T8 groups, respectively. About the main factor effects, both showed non-significant results. Similarly, influence of different treatment diets, in imparting colour to the yolks, was found to be non-significant (p>0.05 at different 28-day time intervals. Further, the average yolk index values ranged non-significantly from 0.360 (T6 to 0.383 (T4. Conclusion: The SPR can be incorporated into layer diet as a source of inorganic as well as organic nutrients without affecting its egg quality characteristics.

  18. Skeletal response to diet with soya bean seeds used as primary source of protein in growing broiler chickens.

    Science.gov (United States)

    Olkowski, B; Charuta, A; Radzki, R; Bieńko, M; Toczko, R

    2016-08-01

    The study was conducted using 120 commercial broiler chicks (Ross 308) randomly allocated to two experimental groups. The experimental diets, differing only in protein source, either solvent-extracted soya bean meal (SBM) or traditional (non-genetically modified) full-fat soya bean seeds (FFS), were prepared using practical corn-based formulation designed to meet nutritional requirements of broilers. Performance parameters were monitored weekly. Also, the subjects were evaluated daily for overt changes in skeletal anatomy and gait physiology. Randomly selected chickens from each group (seven males and seven females) were euthanized at 2, 3, 4 and 6 weeks of age, and bone specimens were collected for further study. Bone mineral density (BMD) and bone mineral content (BMC) were determined in tibiotarsal bones. Broilers fed FFS diet showed retarded growth rate and decreased feed intake (both p chickens from the FFS group in comparison with the SBM group. The chickens fed the FFS diet showed higher incidence of skeletal pathology including angular deformities and torticollis (both p chickens. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

  19. Seasonal and annual variations of metal uptake, bioaccumulation, and toxicity in Trifolium repens and Lolium perenne growing in a heavy metal-contaminated field.

    Science.gov (United States)

    Bidar, Géraldine; Pruvot, Christelle; Garçon, Guillaume; Verdin, Anthony; Shirali, Pirouz; Douay, Francis

    2009-01-01

    The reclamation of nonferrous metal-polluted soil by phytoremediation requires an overall and permanent plant cover. To select the most suitable plant species, it is necessary to study metal effects on plants over the time, thereby checking that metals remain stored in root systems and not transferred to aerial parts. In this purpose, the seasonal and annual variations of metal bioaccumulation, transfer, and phytotoxicity in Trifolium repens and Lolium perenne grown in a Cd-, Pb-, and Zn-contaminated soil were also studied. The experimental site was located near a closed smelter. In spring 2004, two areas were sown with T. repens and L. perenne, respectively. Thereafter, the samplings of plant roots and shoots and surrounding soils were realized in autumn 2004 and spring and autumn 2005. The soil agronomic characteristics, the Cd, Pb, and Zn concentrations in the surrounded soils and plant organs, as well as the oxidative alterations (superoxide dismutase [SOD], malondialdehyde [MDA], and 8-hydroxy-2'-deoxyguanosine [8-OHdG]) in plant organs were carried out. Whatever the sampling period, metal concentrations in soils and plants were higher than background values. Contrary to the soils, the fluctuations of metal concentrations were observed in plant organs over the time. Bioaccumulation and transfer factors confirmed that metals were preferentially accumulated in the roots as follows: Cd>Zn>Pb, and their transfer to shoots was limited. Foliar metal deposition was also observed. The results showed that there were seasonal and annual variations of metal accumulation in the two studied plant species. These variations differed according to the organs and followed nearly the same pattern for the two species. Oxidative alterations were observed in plant organs with regard to SOD antioxidant activities, MDA, and 8-OHdG concentrations. These alterations vary according to the temporal variations of metal concentrations. Metal concentrations in surrounded soils and plant

  20. Effect of pre-grazing herbage mass on dairy cow performance, grass dry matter production and output from perennial ryegrass (Lolium perenne L.) pastures.

    Science.gov (United States)

    Wims, C M; Delaby, L; Boland, T M; O'Donovan, M

    2014-01-01

    A grazing study was undertaken to examine the effect of maintaining three levels of pre-grazing herbage mass (HM) on dairy cow performance, grass dry matter (DM) production and output from perennial ryegrass (Lolium perenne L.) pastures. Cows were randomly assigned to one of three pre-grazing HM treatments: 1150 - Low HM (L), 1400 - Medium HM (M) or 2000 kg DM/ha - High HM (H). Herbage accumulation under grazing was lowest (Ppastures required more grass silage supplementation during the grazing season (+73 kg DM/cow) to overcome pasture deficits due to lower pasture growth rates (Ppasture intake, although cows grazing the L pastures had to graze a greater daily area (Ppasture reduces pasture DM production and at a system level may increase the requirement for imported feed.

  1. Effect of DA-6 and EDTA alone or in combination on uptake, subcellular distribution and chemical form of Pb in Lolium perenne.

    Science.gov (United States)

    He, Shanying; Wu, Qiuling; He, Zhenli

    2013-11-01

    The effects of growth-promoting hormone diethyl aminoethyl hexanoate (DA-6) and EDTA, either alone or in combination applied to original soil or lead (Pb) spiked soil on Pb phytoextraction, subcellular distribution and chemical forms in Lolium perenne were studied. EDTA addition alone significantly reduced plant biomass though it increased Pb accumulation (PDA-6 alone increased both plant biomass and Pb accumulation (PDA-6 being the most effective. DA-6 combined with EDTA compensated the adverse effect of the latter on plant growth, and resulted in a synergistic effect on Pb uptake and translocation, with the maximum accumulation occurring in the EDTA+10μM DA-6 treatment. At the subcellular level, about 35-66% of Pb was distributed in cell wall and 21-42% in soluble fraction, with a minority present in cellular organelles fraction. EDTA addition alone increased the proportion of Pb in soluble and cellular organelles fraction, while DA-6 detoxified Pb in plant by storing additional Pb in cell wall, and 10μM DA-6 was the most effective. Of the total Pb in plant shoot, 27-52% was NaCl extractable, 22-47% HAc extractable, followed by other fractions. Contrary to EDTA, DA-6 significantly decreased Pb migration in plant. These results suggest that Pb fixation by pectates and proteins in cell wall and compartmentalization by vacuole might be responsible for Pb detoxification in plant, and the combined use of EDTA and 10μM DA-6 appears to be optimal for improving the remediation efficiency of L. perenne for Pb contaminated soil. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Effect of organic fertiliser residues from rice production on nitrogen fixation of soya (Glycine max L. Merrill, Chiang Mai 60 variety

    Directory of Open Access Journals (Sweden)

    Nattida Luangmaka

    2013-09-01

    Full Text Available A field study was undertaken on the residual effect of organic fertilisers applied to the preceding rice cropping on nitrogen fixation of soya in a rice-soya cropping system. The experiment was conducted on a farmer’s lowland paddy in Mae Rim district, Chiang Mai province, Thailand. Organic fertiliser treatments assigned were: 1 control (no fertiliser, 2 animal manure of cattle (AM, 3 compost (CP, 4 azolla (AZ, 5 AM + CP, 6 AM + AZ, 7 CP + AZ and 8 AM + CP + AZ. Soya seeds were planted without rhizobial inoculation in December 2011, four months after the application of organic fertilisers. Nodule weight, total shoot nitrogen accumulation and relative ureide index at various growth stages were recorded as the indices of nitrogen fixation. Results of the study demonstrate that the residues from the application the organic fertilisers of narrow C/N ratios during the land preparation for rice cropping four months before soya cultivation promoted nitrogen fixation by native rhizobia.

  3. Desarrollo de un proceso a escala de laboratorio para la obtención de harina y un producto alimenticio a base de ocara de soya

    OpenAIRE

    Hernández González, Juliana; Zapata Narváez, Juliana

    2008-01-01

    La producción de leche de soya genera como subproducto la ocara, un material insoluble compuesto básicamente de proteína, fibra y carbohidratos. Este producto es utilizado en la industria de alimentos para animales por su valor proteínico.

  4. Evaluation of biohydrogenation rate of canola vs. soya bean seeds as unsaturated fatty acids sources for ruminants in situ.

    Science.gov (United States)

    Pashaei, S; Ghoorchi, T; Yamchi, A

    2016-04-01

    An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro-organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  5. Effect of diets containing potato protein or soya bean meal on the incidence of spontaneously-occurring subclinical necrotic enteritis and the physiological response in broiler chickens.

    Science.gov (United States)

    Fernando, P S; Rose, S P; Mackenzie, A M; Silva, S S P

    2011-02-01

    1. An experiment was conducted to compare and explain the incidence of spontaneously occurring subclinical necrotic enteritis in broiler chickens that were fed on two practical broiler diets that differed in the major protein concentrates (soya bean meal or potato protein concentrates) and examine the relationships between the severity of the disease and the growth performance and physiological responses of the chickens. 2. A total of 840, 20-d-old birds were randomly allocated to 12 pens. Two maize-based nutritionally complete diets that either contained some potato protein or soya bean meal as the major protein supplement were fed for 16 d. Twelve birds were randomly sampled from each pen at the end of the feeding period and their blood sampled and intestinal tracts and livers dissected. 3. The birds fed on the potato protein diet had a significantly 7·7% lower feed intake and a significantly 7·8% lower growth rate compared with the birds fed on the soya-based diet. There were no significant differences in feed conversion efficiency or mortality. There were no differences in the determined apparent metabolisable energy concentrations, however, the apparent dry matter digestibility of the potato protein diet was significantly higher than that of the soya based diet and the apparent crude protein digestibility of the potato protein diet was significantly lower. 4. A significantly higher alpha toxin antibody titre was found in the birds fed on the potato protein diet compared with those fed on the soya protein diet. There was a significantly increased incidence of hepatic lesions in the birds fed on the potato protein diet compared with the birds fed on the soya diet. The mean incidence of intestinal necroses tended to be greater in the birds fed on the potato protein diet (23·6%) compared with the birds fed on the soya-based diet (15·3%). 5. There was a significant linear relationship between ileal digesta sialic acid concentration and serum alpha toxin

  6. Evaluación agronómica y nutricional del pasto Ryegrass perenne tetraploide (Lolium perenne producido en lecherías de las zonas altas de Costa Rica. I. Producción de biomasa y fenología

    Directory of Open Access Journals (Sweden)

    Luis Villalobos

    2010-01-01

    3090 msnm, provincia de Cartago. La disponibilidad de materia seca pre y pos-pastoreo, el aprovechamiento por hectárea y su porcentaje de utilización promedio para todas las fincas fueron 4110, 2276 y 1833 kg.ha-1 por corte y 44,82%, respectivamente. La edad fenológica y la relación hoja: tallo promedio de las pasturas de ryegrass perenne fueron 2,84 hojas verdes por rebrote, 56:44. Asimismo, la composición promedio de la pradera fue 76,09% ryegrass, 13,76% otras gramíneas (principalmente pasto kikuyo, 6,02% trébol, 1,25% malezas y 2,88% material senescente, respectivamente. La mayor producción de biomasa durante marzo y abril puede conservarse para utilizarse durante las épocas críticas de disponibilidad de forraje o cuando por razones climatológicas se reduce el número de horas efectivas de pastoreo. La evaluación de la edad fenológica en que se pastorea el pasto ryegrass perenne, indica que las fincas en la zona de estudio utilizan esta gramínea en un estado en el que sus reservas de carbohidratos se han recuperado, lo que permite obtener una producción de biomasa y valor nutricional adecuados para satisfacer las necesidades del ganado lechero de la zona.

  7. Zebrafish ( Danio rerio) as a model for investigating the safety of GM feed ingredients (soya and maize); performance, stress response and uptake of dietary DNA sequences.

    Science.gov (United States)

    Sissener, Nini H; Johannessen, Lene E; Hevrøy, Ernst M; Wiik-Nielsen, Christer R; Berdal, Knut G; Nordgreen, Andreas; Hemre, Gro-Ingunn

    2010-01-01

    A 20-d zebrafish (Danio rerio) feeding trial, in which a near doubling of fish weight was achieved, was conducted with GM feed ingredients to evaluate feed intake, growth, stress response and uptake of dietary DNA. A partial aim of the study was to assess zebrafish as a model organism in GM safety assessments. Roundup Ready soya (RRS), YieldGard Bt maize (MON810) and their non-modified, maternal, near-isogenic lines were used in a 2 x 2 factorial design. Soya variety and maize variety were the main factors, both with two levels; non-GM and GM. Compared with fish fed non-GM maize, those fed GM maize exhibited significantly better growth, had lower mRNA transcription levels of superoxide dismutase (SOD)-1 and a tendency (non-significant) towards lower transcription of heat shock protein 70 in liver. Sex of the fish and soya variety had significant interaction effects on total RNA yield from the whole liver and transcription of SOD-1, suggesting that some diet component affecting males and females differently was present in different levels in the GM and the non-GM soya used in the present study. Dietary DNA sequences were detected in all of the organs analysed, but not all of the samples. Soya and maize rubisco (non-transgenic, multicopy genes) were most frequently detected, while MON810 transgenic DNA fragments were detected in some samples and RRS fragments were not detected. In conclusion, zebrafish shows promise as a model for this application.

  8. Genetic improvement of the shoot architecture and yield in soya bean plants via the manipulation of GmmiR156b.

    Science.gov (United States)

    Sun, Zhengxi; Su, Chao; Yun, Jinxia; Jiang, Qiong; Wang, Lixiang; Wang, Youning; Cao, Dong; Zhao, Fang; Zhao, Qingsong; Zhang, Mengchen; Zhou, Bin; Zhang, Lei; Kong, Fanjiang; Liu, Baohui; Tong, Yiping; Li, Xia

    2018-05-05

    The optimization of plant architecture in order to breed high-yielding soya bean cultivars is a goal of researchers. Tall plants bearing many long branches are desired, but only modest success in reaching these goals has been achieved. MicroRNA156 (miR156)-SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) gene modules play pivotal roles in controlling shoot architecture and other traits in crops like rice and wheat. However, the effects of miR156-SPL modules on soya bean architecture and yield, and the molecular mechanisms underlying these effects, remain largely unknown. In this study, we achieved substantial improvements in soya bean architecture and yield by overexpressing GmmiR156b. Transgenic plants produced significantly increased numbers of long branches, nodes and pods, and they exhibited an increased 100-seed weight, resulting in a 46%-63% increase in yield per plant. Intriguingly, GmmiR156b overexpression had no significant impact on plant height in a growth room or under field conditions; however, it increased stem thickness significantly. Our data indicate that GmmiR156b modulates these traits mainly via the direct cleavage of SPL transcripts. Moreover, we found that GmSPL9d is expressed in the shoot apical meristem and axillary meristems (AMs) of soya bean, and that GmSPL9d may regulate axillary bud formation and branching by physically interacting with the homeobox gene WUSCHEL (WUS), a central regulator of AM formation. Together, our results identify GmmiR156b as a promising target for the improvement of soya bean plant architecture and yields, and they reveal a new and conserved regulatory cascade involving miR156-SPL-WUS that will help researchers decipher the genetic basis of plant architecture. © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  9. Feeding value of whole raw soya beans as a protein supplement for beef cattle consuming low-quality forages.

    Science.gov (United States)

    Arelovich, H M; Lagrange, S; Torre, R; Martinez, M F; Laborde, H E

    2018-02-01

    Experiments (Exp) I and II were conducted to compare raw whole soya beans (WSB), roasted (rWSB) or other protein sources as supplements of low-quality forages fed ad libitum to beef cattle, upon DM intake (DMI), ruminal and blood parameters, and animal performance. Exp I: treatments for wheat straw fed to four ruminally cannulated steers were (i) Control-WS: no supplement; (ii) WSB-WS: whole soya beans; (iii) rWSB-WS: roasted WSB; and (iv) SBM-WS: soybean meal-wheat midds mixture; all fed at 1.4 kg DM/day. Exp II: 12 steers grazed deferred grain sorghum (DS) receiving these treatments: (i) Control-DS: no supplement; (ii) WSB-DS: 1.26 kg DM/day whole soya beans; and (iii) SFM-DS: 1.35 kg DM/day of sunflower meal. In Exp I, WS DMI resulted 47, 52 and 41% greater for WSB-WS, rWSB-WS and SBM-WS, respectively, than Control-WS (p < .05). In Exp II, the DMI of DS was unaffected by supplementation; a substitution of DS by supplement was found for WSB-DS (p < .05); however, total diet and digestible DMI increased with supplementation (p < .05). Rumen pH in Exp I remained unaffected by supplementation, but N-NH 3 as well as blood urea-N in Exp II increased (p < .05). In Exp II, average daily weight gains improved similarly with both supplements compared with Control-DS. Additionally, feed-to-gain ratio decreased (p < .05), being lower for WSB-DS (8.3) vs. SFM-DS (9.9). Roasting effects of WSB as a supplement for low-quality forages were not detected, and all protein sources increased total diet DMI and forage utilization. Only moderate cattle weight gains could be expected for unsupplemented DS. © 2017 Blackwell Verlag GmbH.

  10. Effects of isoflavone-containing soya protein on ex vivo cholesterol efflux, vascular function and blood markers of CVD risk in adults with moderately elevated blood pressure: a dose-response randomised controlled trial.

    Science.gov (United States)

    Richter, Chesney K; Skulas-Ray, Ann C; Fleming, Jennifer A; Link, Christina J; Mukherjea, Ratna; Krul, Elaine S; Kris-Etherton, Penny M

    2017-05-01

    Emerging CVD risk factors (e.g. HDL function and central haemodynamics) may account for residual CVD risk experienced by individuals who meet LDL-cholesterol and blood pressure (BP) targets. Recent evidence suggests that these emerging risk factors can be modified by polyphenol-rich interventions such as soya, but additional research is needed. This study was designed to investigate the effects of an isoflavone-containing soya protein isolate (delivering 25 and 50 g/d soya protein) on HDL function (i.e. ex vivo cholesterol efflux), macrovascular function and blood markers of CVD risk. Middle-aged adults (n 20; mean age=51·6 (sem 6·6) years) with moderately elevated brachial BP (mean systolic BP=129 (sem 9) mmHg; mean diastolic BP=82·5 (sem 8·4) mmHg) consumed 0 (control), 25 and 50 g/d soya protein in a randomised cross-over design. Soya and control powders were consumed for 6 weeks each with a 2-week compliance break between treatment periods. Blood samples and vascular function measures were obtained at baseline and following each supplementation period. Supplementation with 50 g/d soya protein significantly reduced brachial diastolic BP (-2·3 mmHg) compared with 25 g/d soya protein (Tukey-adjusted P=0·03) but not the control. Soya supplementation did not improve ex vivo cholesterol efflux, macrovascular function or other blood markers of CVD risk compared with the carbohydrate-matched control. Additional research is needed to clarify whether effects on these CVD risk factors depend on the relative health of participants and/or equol producing capacity.

  11. Casein and soya-bean protein have different effects on whole body protein turnover at the same nitrogen balance

    DEFF Research Database (Denmark)

    Nielsen, K; Kondrup, J; Elsner, Petteri

    1994-01-01

    was recovered from urinary ammonia and urea during isotope steady state for measurement of protein synthesis and protein degradation. Compared with starvation the protein-free diet decreased N excretion by 75%, probably by increasing the rate of reutilization of amino acids from endogenous proteins for protein......The present study examined whether different proteins have different effects on whole-body protein turnover in adult rats. The rats were either starved, given a protein-free but energy-sufficient diet (1 MJ/kg body weight (BW) per d) or a diet containing intact casein, hydrolysed casein......, or hydrolysed soya-bean protein at a level of 9.1 g/kg BW per d. The diets, which were isoenergetic with the same carbohydrate: fat ratio, were given as a continuous intragastric infusion for at least 4 d. During the last 19 h 15N-glycine (a primed continuous infusion) was given intragastrically and 15N...

  12. Soil erosion and mitigation measures on rented and owned fields in Uruguay: the impact of transgenic soya and foreign investors

    Science.gov (United States)

    Caon, Lucrezia; Kessler, Aad; Keesstra, Saskia; Cruze, Rick

    2014-05-01

    Governments, companies and individuals with financial capital to invest, are worldwide buying or renting land in developing or third world countries. Uruguay is a developing country whose economy is mainly based on agriculture. Since 2000 many foreigners started to invest in the Uruguayan agricultural sector and to practice intensive large-scale agriculture. The significant presence of foreigners in the country is proven by the fact that almost 360 000 ha out of the 500 000 ha forming the study area were managed by foreigners in 2012. Nowadays farmers have abandoned the traditional crop rotation plan that included pasture to produce grain for export, and transgenic soya (soya RR) became the main crop planted by both foreigners and locals. Besides the high soil erosion rates related to having soya as main crop, planting soya implies the use of glyphosate, a broad-spectrum systemic herbicide leading to important environmental impacts. It is commonly said that foreigners investing in poor countries are exploiting the local natural resources aiming to get the highest possible profit from them. Is this a valid assumption in Uruguay? The purpose of this study was to compare the land management style of foreign and local farmers and to relate it to the soil erosion occurring in the study area. The land tenure (rented or owned fields) and the type of farmer interviewed ("individual farmer" equivalent to L.L.C. or "anonymous society" equivalent to P.L.C.) were taken into consideration during the analysis. Based on what stated by the farmers interviewed, the soil erosion simulations considered the seven most popular crop rotation plans on rented and owned fields, three ideals crop rotation plans, the application of no mitigation measures, and the construction of terraces and conservation buffers. Depending on the crop rotation plan, soils characterized by slope gradients higher than 2 resulted in soil erosion rates higher than the 7 ton/ha/year allowed by law. The highest

  13. A dynamic growth model of vegetative soya bean plants: model structure and behaviour under varying root temperature and nitrogen concentration

    Science.gov (United States)

    Lim, J. T.; Wilkerson, G. G.; Raper, C. D. Jr; Gold, H. J.

    1990-01-01

    A differential equation model of vegetative growth of the soya bean plant (Glycine max (L.) Merrill cv. Ransom') was developed to account for plant growth in a phytotron system under variation of root temperature and nitrogen concentration in nutrient solution. The model was tested by comparing model outputs with data from four different experiments. Model predictions agreed fairly well with measured plant performance over a wide range of root temperatures and over a range of nitrogen concentrations in nutrient solution between 0.5 and 10.0 mmol NO3- in the phytotron environment. Sensitivity analyses revealed that the model was most sensitive to changes in parameters relating to carbohydrate concentration in the plant and nitrogen uptake rate.

  14. Extraction methods and test techniques for detection of vegetable proteins in meat products. I. Qualitative detection of soya derivatives.

    Science.gov (United States)

    Hyslop, N S

    1976-06-01

    Extracts of 3 soya bean preparations, used commercially in certain countries to replace part of the meat in popular meat products, were made by treatment with (i) sodium dodecyl sulphate, (ii) Triton-X100 or (iii) n-Butanol. Similar extracts were made from beef and pork. All extracts were examined by electrophoretic and immunological techniques. Stained polyacrylamide gels revealed distinctive protein bands after electrophoresis. The migration rates of corresponding bands differed between beef and pork extracts. However, the migration rates of vegetable bands revealed certain similarities, but differed very greatly from those of animal origin. Characteristic fast-migrating S-bands were distinguishable only in extracts of vegetable protein. Immunodiffusion tests, using antisera produced in rabbits against each extract, revealed varying degrees of similarity between extracts of vegetable origin, but the antisera were specific for either vegetable or animal protein.

  15. Signatures of soft sweeps across the Dt1 locus underlying determinate growth habit in soya bean [Glycine max (L.) Merr.].

    Science.gov (United States)

    Zhong, Limei; Yang, Qiaomei; Yan, Xin; Yu, Chao; Su, Liu; Zhang, Xifeng; Zhu, Youlin

    2017-09-01

    Determinate growth habit is an agronomically important trait associated with domestication in soya bean. Previous studies have demonstrated that the emergence of determinacy is correlated with artificial selection on four nonsynonymous mutations in the Dt1 gene. To better understand the signatures of the soft sweeps across the Dt1 locus and track the origins of the determinate alleles, we examined patterns of nucleotide variation in Dt1 and the surrounding genomic region of approximately 800 kb. Four local, asymmetrical hard sweeps on four determinate alleles, sized approximately 660, 120, 220 and 150 kb, were identified, which constitute the soft sweeps for the adaptation. These variable-sized sweeps substantially reflected the strength and timing of selection and indicated that the selection on the alleles had been completed rapidly within half a century. Statistics of EHH, iHS, H12 and H2/H1 based on haplotype data had the power to detect the soft sweeps, revealing distinct signatures of extensive long-range LD and haplotype homozygosity, and multiple frequent adaptive haplotypes. A haplotype network constructed for Dt1 and a phylogenetic tree based on its extended haplotype block implied independent sources of the adaptive alleles through de novo mutations or rare standing variation in quick succession during the selective phase, strongly supporting multiple origins of the determinacy. We propose that the adaptation of soya bean determinacy is guided by a model of soft sweeps and that this model might be indispensable during crop domestication or evolution. © 2017 The Authors. Molecular Ecology Published by John Wiley & Sons Ltd.

  16. Influence of water activity and temperature on growth and mycotoxin production by Alternaria alternata on irradiated soya beans.

    Science.gov (United States)

    Oviedo, Maria Silvina; Ramirez, Maria Laura; Barros, Germán Gustavo; Chulze, Sofia Noemi

    2011-09-15

    The aim of this study was to determine the effects of water activity (a(w)) (0.99-0.90), temperature (15, 25 and 30°C) and their interactions on growth and alternariol (AOH) and alternariol monomethyl ether (AME) production by Alternaria alternata on irradiated soya beans. Maximum growth rates were obtained at 0.980 a(w) and 25°C. Minimum a(w) level for growth was dependent on temperature. Both strains were able to grow at the lowest a(w) assayed (0.90). Maximum amount of AOH was produced at 0.98 a(w) but at different temperatures, 15 and 25°C, for the strains RC 21 and RC 39 respectively. Maximum AME production was obtained at 0.98 a(w) and 30°C for both strains. The concentration range of both toxins varied considerably depending on a(w) and temperature interactions. The two metabolites were produced over the temperature range 15 to 30°C and a(w) range 0.99 to 0.96. The limiting a(w) for detectable mycotoxin production is slightly greater than that for growth. Two-dimensional profiles of a(w)× temperature were developed from these data to identify areas where conditions indicate a significant risk from AOH and AME accumulation on soya bean. Knowledge of AOH and AME production under marginal or sub-optimal temperature and a(w) conditions for growth can be important since improper storage conditions accompanied by elevated temperature and moisture content in the grain can favour further mycotoxin production and lead to reduction in grain quality. This could present a hazard if the grain is used for human consumption or animal feedstuff. Copyright © 2011 Elsevier B.V. All rights reserved.

  17. Corrección de la clorosis férrica con quelato EDDHA en cultivos sembrados en suelo alcalino y calcáreo

    Directory of Open Access Journals (Sweden)

    Arturo Díaz Franco

    2018-01-01

    Full Text Available La deficiencia de hierro (Fe en los cultivos se manifiesta principalmente en regiones con suelos alcalinos y calcáreos. En el presente estudio se evaluó la efectividad del Fe3+ quelatado con EDDHA [ac. etilendiamino di-(o-hidroxifenilacetato] y la aspersión foliar de FeSO4 solos y combinados, en los cultivos de sorgo, soya, okra y frijol. El EDDHA-Fe (Ultraferro® se aplicó a dosis de 1% en la siembra sobre las semillas y para el FeSO4 se hicieron dos aplicaciones foliares al 1.5%. En los cuatro experimentos se determinó el índice de clorofila (SPAD en tres fechas (15 y 28 de marzo, 6 de abril, y a los 48-50 días después de la siembra se cuantificó la biomasa foliar y radical. Entre los cultivos, las plantas de soya y okra manifestaron la mayor intensidad de clorosis férrica, la cual fue visible entre los tratamientos. En ambos cultivos los mayores valores SPAD y de biomasa foliar y radical se obtuvieron con la aplicación de EDDHA-Fe solo o combinado con FeSO4. En frijol y sorgo, la clorosis fue menos visible, para las plantas de frijol el mayor índice de clorofila se registró con EDDHA-Fe y FeSO4 en las dos últimas fechas de evaluación (28 de marzo y 6 de abril y los tres tratamientos químicos superaron de forma similar al testigo, en la biomasa foliar y radical. Las plantas de sorgo registraron los mayores índices SPAD en la última fecha (6 de abril, mientras que no fueron significativas las diferencias en la biomasa foliar y radical. No se observó efecto aditivo en las variables con la combinación EDDHA-Fe y FeSO4. En general, los resultados indican que la aplicación de EDDHA-Fe solo o combinado con FeSO4 foliar en soya, okra y frijol, mostraron corrección de la clorosis férrica de manera efectiva.

  18. Teleseismic SKS splitting beneath East Antarctica using broad-band stations around Soya Coast

    Science.gov (United States)

    Usui, Y.; Kanao, M.

    2006-12-01

    We observed shear wave splitting of SKS waves from digital seismographs that are recorded at 5 stations around Soya Coast in the Lutzow-Holm Bay, East Antarctica. Their recording systems are composed of a three-component broadband seismometer (CMG-40T), a digital recording unit and a solar power battery supply. The events used were selected from 1999 to 2004 and phase arrival times were calculated using the IASPEI91 earth model (Kennet, 1995). In general, we chose the data from earthquakes with m>6.0 and a distance range 85° < Δ < 130° for the most prominent SKS waves We used the methods of Silver and Chan (1991) for the inversion of anisotropy parameters and estimated the splitting parameters φ (fast polarization direction) and δt (delay time between split waves) assuming a single layer of hexagonal symmetry with a horizontal symmetry axis. The weighted averages of all splitting parameters (φ, δt) for each station are AKR (30±4, 1.30±0.2), LNG (58±6, 1.27±0.2), SKL (67±10, 0.94±0.2), SKV (40±6, 1.28±0.3) and TOT (52±8, 1.26±0.3), where the weights are inversely proportional to the standard deviations for each solution. As compared to typical delay times of SKS waves which show 1.2s (Silver and Chan 1991; Vinnik et al., 1992), the result shows generally the same value. In previous study, Kubo and Hiramatsu (1998) estimate the splitting parameter for Syowa station (SYO), where is located near our using stations in East Antarctica, and the results are (49±3, 0.70±0.1). Although it is consistent with our results for fast polarization direction, δt for our results are large relatively to those of SYO. The difference may be due to either different incident angle or more complex anisotropic structure. We found that fast polarization direction is systematically parallel to coast line in the Lutzow-Holm Bay, East Antarctica, which is consistent with NE-SW paleo compressional stress. The absolute plate motion based on the HS2-NUVEL1 (Gripp and Gordon

  19. Ameliorating effect of olive oil on fertility of male rats fed on genetically modified soya bean

    Directory of Open Access Journals (Sweden)

    Thanaa A. F. El-Kholy

    2015-09-01

    Full Text Available Background: Genetically modified soya bean (GMSB is a commercialized food. It has been shown to have adverse effects on fertility in animal trials. Extra virgin olive oil (EVOO has many beneficial effects including anti-oxidant properties. The aim of this study is to elucidate if addition of EVOO ameliorates the adverse effects on reproductive organs of rats fed on GMSB containing diet. Methods: Forty adult male albino rats (150–180 g of Sprague Dawley strain were separated into four groups of 10 rats each: Group 1 – control group fed on basal ration, Group 2 – fed on basal ration mixed with EVOO (30%, Group 3 – fed on basal ration mixed with GMSB (15%, and Group 4 – fed on basal ration mixed with GMSB (15% and EVOO (30%. This feeding regimen was administered for 65 days. Blood samples were collected to analyze serum zinc, vitamin E, and testosterone levels. Histopathological and weight changes in sex organs were evaluated. Results: GMSB diet reduced weight of testis (0.66±0.06 vs. 1.7±0.06, p<0.001, epididymis (0.489±0.03 vs. 0.7±0.03, p<0.001, prostate (0.04±0.009 vs. 0.68±0.04, p<0.001, and seminal vesicles (0.057±0.01 vs. 0.8±0.04, p<0.001. GMSB diet adversely affected sperm count (406±7.1 vs. 610±7.8, p<0.001, motility (p<0.001, and abnormality (p<0.001. GMSB diet also reduced serum zinc (p<0.05, vitamin E (p<0.05, and testosterone (p<0.05 concentrations. EVOO diet had no detrimental effect. Addition of EVOO to GMSB diet increased the serum zinc (p<0.05, vitamin E (p<0.05, and testosterone (p<0.05 levels and also restored the weights of testis (1.35±0.16 vs. 0.66±0.06, p<0.01, epididymis (0.614±0.13 vs. 0.489±0.03, p<0.001, prostate (0.291±0.09 vs. 0.04±0.009, p<0.001, seminal vesicle (0.516±0.18 vs. 0.057±0.01, p<0.001 along with sperm count (516±3.1 vs. 406±7.1, p<0.01, motility (p<0.01, and abnormality (p<0.05. Conclusion: EVOO ameliorates the adverse effects of GMSB on reproductive organs in adult male

  20. A Simulation Model of Mesophytic Perennial Grasslands Un Modelo de Simulación de Pradera Perenne Mesofítica

    Directory of Open Access Journals (Sweden)

    Giorgio Castellaro G

    2012-09-01

    Full Text Available Grasslands are complex ecosystems and their processes are affected by soil, meteorological, and management variables. In this context, dynamic simulation models are useful to understand these processes and to design grassland use strategies. The aim of this study was to develop and validate a simulation model of perennial pasture growth based on soil and climate variables. A first approach considered that soil fertility levels were adequate; therefore, soil water availability and phytomass level were the main variables affecting pasture growth. The subroutines considered were water balance, pasture growth, and root biomass dynamics. The hypotheses regarding the functioning of the system were formulated as a group of equations which were solved numerically with a program written in Visual Basic®. Model validation was performed by statistical comparison between simulated DM and DM obtained from experiments conducted in Valdivia (39°47' S., 73°15' W; 9 m a.s.l.. In these experiments we measured DM accumulation on naturalized grassland and ryegrass (Lolium perenne L.-white clover (Trifolium repens L. pastures under frequent defoliation. Soil data, temperature, solar radiation, and rainfall were obtained from a meteorological station located in Valdivia. The coefficient of determination between simulated values and those measured in the experiments were higher in the DM accumulation (R² = 98% simulations. When pasture was subjected to frequent defoliation, the degree of fit of the model was lower (R² = 60%; however, the model was able to predict the trend in the data.Los pastizales son ecosistemas complejos y sus procesos se ven afectados por variables edáficas, meteorológicas y de manejo. En este contexto, los modelos dinámicos de simulación son utiles para la comprensión de estos procesos y disenar estrategias de utilización de las praderas. El objetivo de este trabajo fue elaborar y validar un modelo de simulación de crecimiento de

  1. Combined impact of heavy metals (Pb2+ and Cd2+ and salinity on the condition of Lolium perenne long-term assimilation apparatus

    Directory of Open Access Journals (Sweden)

    V. P. Bessonova

    2014-12-01

    Full Text Available Contamination of soil and atmosphere by heavy metals negatively affects physiological and biochemical processes in plants. The objective of this study is to analyze the combined impact of heavy metals Pb2+ and Cd2+ on the background of salinity on the surface of assimilation and the state of stomata device and the content of plastid pigments in leaves of Lolium perenne L. Decrease in the area of plant leaves on the background of the impact of pollutants has been determined. By the degree of increase of negative impact on this index, variants of the experience can be arranged as follows: Pb2+ + Cd2+ < NaCl < Pb2+ + Cd2+ + NaCl. The investigated factors have the strongest impact on the area of assimilation surface. The reason for its significant decrease in plants of studied variants compared to the control is that both suppression of growth and reduction of leaves area occurs, along with the inhibition of their formation. The damaging effect of sodium chloride is also traced in drying of leaf tips, the latter become lighter in color and some of them get yellow. Under the action of heavy metals and salinity decrease in the leaf index for L. perenne is observed, especially in case of combined actions of toxins, and this rate varies more significantly than the other ones. Salinization of growing substrate significantly reduces the number of stomata on the underside of the leaf epidermis. On the contrary, under the influence of heavy metals their number increases, and under the action of Pb2+ + Cd2+ on the background of chloride salinity it becomes even higher compared with the variant where heavy metals only affect the plants. The length of guard cells of the stomata in the variants with metals in NaCl remains practically unchanged compared with the control, but at joint action it is reduced. The width of stomatal pore in the variants differs insignificantly. The same is applied to the length, except for variants where the plants were exposed

  2. Paciente con esquizofrenia tratado con ziprasidona + clozapina

    Directory of Open Access Journals (Sweden)

    Pol Yanguas E.

    2013-05-01

    Full Text Available P es un paciente diagnosticado de esquizofrenia, sigue en un piso tutelado un programa de rehabilitación, está medicado con clozapina 500 mg/día y ziprasidona 280 mg/ día. Padece hipercolesterolemia, tabaquismo y sus hábitos alimenticios no son buenos. La medicación que utiliza desde 2007 hasta ahora se refleja en la tabla 1. El último tratamiento se le introdujo el 7 de agosto de 2012, habiendo presentado un electro cardiograma (ECG normal, pero con ligera taquicardia ventricular y prolactinemia de 44,8 ng/ml (valores normales: 2-18 ng/ml.

  3. Entre lo perenne y lo efímero: la enseñanza de la arquitectura

    OpenAIRE

    Blas Gomez, Felisa de

    2008-01-01

    Una de las transformaciones en que nos hallamos inmersos en estos últimos años es, la atención a los sentidos, a las propiedades hápticas, ópticas y acústicas del espacio. El cuerpo vuelve a estar amparado mientras se demanda el contacto continuo con la imagen cinética de refracción instantánea. Una necesidad de esencia orgánica, paradójicamente de carácter abstracto. Esta transformación permite renovar el discurso acerca de la frontera entre disciplinas autónomas como la arquitectura y la es...

  4. Genetic variability induced in winter rye (Secale cereale l.), soya bean (Glicine max merill.) and china aster (Callistephus chinensis L. Nees)

    International Nuclear Information System (INIS)

    Muszynski, S.; Darlewska, M.; Dobosz-Rojewska, K.; Wosinska, A.

    1998-01-01

    Optimal treatments for mutation induction were established as follows: for China aster - treating seeds with gamma rays of 6-9 rad; for soya bean - irradiating seeds with fast neutrons of 100 rad, for rye - soaking the grains in water solutions of NEU (0.04% concentration) and of SA (1.5-2.0-2.5 mM concentration). The most interesting mutants of each species are kept in collection. They include the following mutants of China aster (homogamic) of soya bean (with tall and strong stems, with great leaves) and of winter rye (short-straw mutants, xanthina, with horizontal type of growth and with late senescence, combined with long spikes). (author)

  5. An Observation of Diamond-Shaped Particle Structure in a Soya Phosphatidylcohline and Bacteriorhodopsin Composite Langmuir Blodgett Film Fabricated by Multilayer Molecular Thin Film Method

    Science.gov (United States)

    Tsujiuchi, Y.; Makino, Y.

    A composite film of soya phosphatidylcohline (soya PC) and bacteriorhodopsin (BR) was fabricated by the multilayer molecular thin film method using fatty acid and lipid on a quartz substrate. Direct Force Microscopy (DFM), UV absorption spectra and IR absorption spectra of the film were characterized on the detail of surface structure of the film. The DFM data revealed that many rhombus (diamond-shaped) particles were observed in the film. The spectroscopic data exhibited the yield of M-intermediate of BR in the film. On our modelling of molecular configuration indicate that the coexistence of the strong inter-molecular interaction and the strong inter-molecular interaction between BR trimmers attributed to form the particles.

  6. The accumulation of 137Cs of Chernobyl's accident by different soya varieties and the influence of chronic irradiation on the photosynthetic process

    International Nuclear Information System (INIS)

    Goncharova, N.; Zhebrakova, I.; Davydenko, O.; Russkikh, I.

    1996-01-01

    The present studies have confirmed the relations of 137 Cs distribution in legume organs and revealed the behaviour of the soya crops new for Belarus in accumulation of the radionuclide. The greatest part of 137 Cs is concentrated in root systems but its distribution in the organs is variety-related. In plants grown in soil with different radionuclide contents variety-related differences in the chlorophyll contents have been found in different soya varieties, but these differences were independent of the 137 Cs level in the soil. It is found that all six tested varieties grown in the radionuclide contaminated zone of study had disturbances of the reproductive functions and had viable seeds of the first reproduction. The present data goes well with the present concepts in the search of the ways of using contaminated areas to obtain useful production. (author)

  7. Influence of source and micronization of soya bean meal on growth performance, nutrient digestibility and ileal mucosal morphology of Iberian piglets.

    Science.gov (United States)

    Berrocoso, J D; Cámara, L; Rebollar, P G; Guzmán, P; Mateos, G G

    2014-04-01

    The effects of inclusion in the diet of different sources of soya bean meal (SBM) on growth performance, total tract apparent digestibility (TTAD) and apparent ileal digestibility (AID) of major dietary components and mucosal ileum morphology were studied in Iberian pigs weaned at 30 days of age. From 30 to 51 days of age (phase I), there was a control diet based on regular soya bean meal (R-SBM; 44% CP) of Argentina (ARG) origin and five extra diets in which a high-protein soya bean meal (HP-SBM; 49% CP) of the USA or ARG origin, either ground (990 μm) or micronized (60 μm), or a soya protein concentrate (SPC; 65% CP) substituted the R-SBM. From 51 to 61 days of age (phase II), all pigs were fed a common commercial diet in mash form. The following pre-planned orthogonal contrasts were conducted: (1) R-SBM v. all the other diets, (2) SPC v. all the HP-SBM diets, (3) micronized HP-SBM v. ground HP-SBM, (4) HP-SBM of ARG origin v. HP-SBM of US origin and (5) interaction between source and the degree of grinding of the HP-SBM. Dietary treatment did not affect growth performance of the pigs at any age but from 30 to 51 days of age, post weaning diarrhoea (PWD) was higher (Psoya products (micronized SBM or SPC) in substitution of the R-SBM increased the TTAD of all nutrients and reduced PWD but had no advantage in terms of growth performance over the use of ground HP-SBM.

  8. Analysis of peanuts and soya irradiated: is it possible to identify them?; Amendoim e soja irradiados: e possivel identifica-los?

    Energy Technology Data Exchange (ETDEWEB)

    Leal, Alexandre S. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, MG (Brazil)]. E-mail: asleal@cdtn.br; Vieira, Ionara F.R.; Tambourgi, Elias B. [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia Quimica]. E-mail: elias@desq.feq.unicamp.br; ionarafernanda@terra.com.br; Krambrock, Klaus [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Fisica]. E-mail: klaus@fisica.ufmg.br; Prado, Guilherme [ Fundacao Ezequiel Dias (FUNED), Belo Horizonte, MG (Brazil). Div. de Vigilancia Sanitaria]. E-mail: gui@funed.mg.gov.br

    2005-07-01

    In this work we present the results of the electronic paramagnetic resonance (EPR) analysis of peanuts and soya treated by gamma-irradiation. The dose employed varying from 1 to 30 kGy. The results obtained confirm that the EPR method is efficient to detect if the products were irradiated. In general, the EPR signal intensity increases according to the dose until saturation is reached making it possible to determine the dose level. (author)

  9. Micro-relief distribution of major mosses in ice-free areas along the Soya Coast, the Syowa Station area, East Antarctica

    OpenAIRE

    Okitsu,Susumu; Imura,Satoshi; Ayukawa,Eri

    2004-01-01

    Micro-relief distribution of five major mosses, Pottia heimii, Ceratodon purpureus, Bryum pseudotriquetrum, Grimmia lawiana and Bryum argenteum, in the ice-free areas along the Soya Coast, continental part of East Antarctica have been compared. Those five mosses showed three different types of micro-relief distribution, at three different types of sites: mounds, slopes and hollows. Pottia heimii and Ceratodon purpureus prevailed on mound sites mixed with cyanobacteria. In dryer mound environm...

  10. Effect of in situ soil amendments on arsenic uptake in successive harvests of ryegrass (Lolium perenne cv Elka) grown in amended As-polluted soils

    International Nuclear Information System (INIS)

    Hartley, William; Lepp, Nicholas W.

    2008-01-01

    Several iron-bearing additives, selected for their potential ability to adsorb anions, were evaluated for their effectiveness in attenuation of arsenic (As) in three soils with different sources of contamination. Amendments used were lime, goethite (α-FeOOH) (crystallised iron oxide) and three iron-bearing additives, iron grit, Fe II and Fe III sulphates plus lime, applied at 1% w/w. Sequential extraction schemes conducted on amended soils determined As, Cu, Zn and Ni fractionation. Plant growth trials using perennial ryegrass (Lolium perenne var. Elka) assessed shoot As uptake. This was grown in the contaminated soils for 4 months, during which time grass shoots were successively harvested every 3 weeks. Goethite increased biomass yields, but clear differences were observed in As transfer rates with the various iron oxides. In conclusion, whilst Fe-oxides may be effective in situ amendments, reducing As bioavailability, their effects on plant growth require careful consideration. Soil-plant transfer of As was not completely halted by any amendment. - Arsenic attenuation is illustrated using Fe-based amendments, their efficacy providing different indicators of success

  11. Effect of ryegrass (Lolium perenne L.) roots inoculation using different arbuscular mycorrhizal fungi (AMF) species on sorption of iron-cyanide (Fe-CN) complexes

    Science.gov (United States)

    Sut, Magdalena; Boldt-Burisch, Katja; Raab, Thomas

    2016-04-01

    Soils and groundwater on sites of the former Manufactured Gas Plants (MGPs) are contaminated with various complex iron-cyanides (Fe-CN). Phytoremediation is a promising tool in stabilization and remediation of Fe-CN affected soils, however, it can be a challenging task due to extreme adverse and toxic conditions. Phytoremediation may be enhanced via rhizosphere microbial activity, which can cooperate on the degradation, transformation and uptake of the contaminants. Recently, increasing number of scientist reports improved plants performance in the removal of toxic compounds with the support of arbuscular mycorrhizae fungi (AMF). Series of batch experiments using potassium hexacyanoferrate (II) solutions, in varying concentrations, were used to study the effect of ryegrass roots (Lolium perenne L.) inoculation with Rhizophagus irregularis and a mixture of Rhizophagus irregularis, Funneliformis mosseae, Rhizophagus aggregatus, and Claroideoglomus etunicatum on Fe-CN sorption. Results indicated significantly higher colonization of R. irregularis than for the mixture of AMF species on ryegrass roots. Sorption experiments revealed significantly higher reduction of total CN and free CN content in the mycorrhizal roots, indicating greater cyanide decrease in the treatment inoculated with R. irregularis. Our study indicates contribution of AM fungi in phytoremediation of Fe-CN contaminated soil.

  12. Arbuscular mycorrhiza enhanced arsenic resistance of both white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.) plants in an arsenic-contaminated soil

    International Nuclear Information System (INIS)

    Dong Yan; Zhu Yongguan; Smith, F. Andrew; Wang Youshan; Chen Baodong

    2008-01-01

    In a compartmented cultivation system, white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.), with their roots freely intermingled, or separated by 37 μm nylon mesh or plastic board, were grown together in an arsenic (As) contaminated soil. The influence of AM inoculation on plant growth, As uptake, phosphorus (P) nutrition, and plant competitions were investigated. Results showed that both plant species highly depended on mycorrhizas for surviving the As contamination. Mycorrhizal inoculation substantially improved plant P nutrition, and in contrast markedly decreased root to shoot As translocation and shoot As concentrations. It also showed that mycorrhizas affected the competition between the two co-existing plant species, preferentially benefiting the clover plants in term of nutrient acquisition and biomass production. Based on the present study, the role of AM fungi in plant adaptation to As contamination, and their potential use for ecological restoration of As contaminated soils are discussed. - Both white clover and ryegrass highly depend on the mycorrhizal associations for surviving heavy arsenic contamination

  13. Human immune responsiveness to Lolium perenne pollen allergen Lol p III (rye III) is associated with HLA-DR3 and DR5.

    Science.gov (United States)

    Ansari, A A; Freidhoff, L R; Meyers, D A; Bias, W B; Marsh, D G

    1989-05-01

    A well-characterized allergen of Lolium perenne (perennial rye grass) pollen, Lol p III, has been used as a model antigen to study the genetic control of the human immune response. Associations between HLA type and IgE or IgG antibody (Ab) responsiveness to Lol p III were studied in two groups of skin-test-positive Caucasoid adults (N = 135 and 67). We found by nonparametric and parametric analyses that immune responsiveness to Lol p III was significantly associated with HLA-DR3 and DR5. No association was found between any DQ type and immune responsiveness to Lol p III. Geometric mean IgE or IgG Ab levels to Lol p III were not different between B8+, DR3+ subjects and B8-, DR3+ subjects, showing that HLA-B8 had no influence on the association. Lol p III IgG Ab data obtained on subjects after grass antigen immunotherapy showed that 100% of DR3 subjects and 100% of DR5 subjects were Ab+. A comparison of all the available protein sequences of DRB gene products showed that the first hypervariable region of DR3 and DR5 (and DRw6), and no other region, contains the sequence Glu9-Tyr-Ser-Thr-Ser13. Our observations are consistent with the possibility that immune responsiveness to the allergen Lol p III is associated with this amino acid sequence in the first hypervariable region of the DR beta 1 polypeptide chain.

  14. Influence of fly ash aided phytostabilisation of Pb, Cd and Zn highly contaminated soils on Lolium perenne and Trifolium repens metal transfer and physiological stress

    International Nuclear Information System (INIS)

    Lopareva-Pohu, Alena; Verdin, Anthony; Garcon, Guillaume; Lounes-Hadj Sahraoui, Anissa; Pourrut, Bertrand; Debiane, Djouher; Waterlot, Christophe; Laruelle, Frederic

    2011-01-01

    Due to anthropogenic activities, large extends of soils are highly contaminated by Metal Trace Element (MTE). Aided phytostabilisation aims to establish a vegetation cover in order to promote in situ immobilisation of trace elements by combining the use of metal-tolerant plants and inexpensive mineral or organic soil amendments. Eight years after Coal Fly Ash (CFA) soil amendment, MTE bioavailability and uptake by two plants, Lolium perenne and Trifolium repens, were evaluated, as some biological markers reflecting physiological stress. Results showed that the two plant species under study were suitable to reduce the mobility and the availability of these elements. Moreover, the plant growth was better on CFA amended MTE-contaminated soils, and the plant sensitivity to MTE-induced physiological stress, as studied through photosynthetic pigment contents and oxidative damage was lower or similar. In conclusion, these results supported the usefulness of aided phytostabilisation of MTE-highly contaminated soils. - Highlights: → Aided phytostabilisation aims to establish a vegetation cover in order to promote immobilisation of MTE. → 8 years after the soil amendments, a pot culture study was carried out in greenhouse conditions. → MTE bioavailability and uptake by the two plants was drastically decreased with amendments. → Our results support the usefulness of aided phytostabilisation of MTE-highly contaminated soils. → CFA addition contributed to the reduction of the MTE mobility and availability for the plants. - Efficiency of Coal Fly Ash amendment for phytostabilisation of Pb, Cd and Zn in MTE-highly contaminated soils.

  15. Arbuscular mycorrhiza enhanced arsenic resistance of both white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.) plants in an arsenic-contaminated soil

    Energy Technology Data Exchange (ETDEWEB)

    Dong Yan; Zhu Yongguan [Department of Soil Environmental Science, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085 (China); Smith, F. Andrew [Soil and Land Systems, School of Earth and Environmental Sciences, Waite Campus, University of Adelaide, Adelaide, SA 5005 (Australia); Wang Youshan [Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry, Beijing 100089 (China); Chen Baodong [Department of Soil Environmental Science, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085 (China)], E-mail: bdchen@rcees.ac.cn

    2008-09-15

    In a compartmented cultivation system, white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.), with their roots freely intermingled, or separated by 37 {mu}m nylon mesh or plastic board, were grown together in an arsenic (As) contaminated soil. The influence of AM inoculation on plant growth, As uptake, phosphorus (P) nutrition, and plant competitions were investigated. Results showed that both plant species highly depended on mycorrhizas for surviving the As contamination. Mycorrhizal inoculation substantially improved plant P nutrition, and in contrast markedly decreased root to shoot As translocation and shoot As concentrations. It also showed that mycorrhizas affected the competition between the two co-existing plant species, preferentially benefiting the clover plants in term of nutrient acquisition and biomass production. Based on the present study, the role of AM fungi in plant adaptation to As contamination, and their potential use for ecological restoration of As contaminated soils are discussed. - Both white clover and ryegrass highly depend on the mycorrhizal associations for surviving heavy arsenic contamination.

  16. Nitrogen deficiency inhibits leaf blade growth in Lolium perenne by increasing cell cycle duration and decreasing mitotic and post-mitotic growth rates.

    Science.gov (United States)

    Kavanová, Monika; Lattanzi, Fernando Alfredo; Schnyder, Hans

    2008-06-01

    Nitrogen deficiency severely inhibits leaf growth. This response was analysed at the cellular level by growing Lolium perenne L. under 7.5 mM (high) or 1 mM (low) nitrate supply, and performing a kinematic analysis to assess the effect of nitrogen status on cell proliferation and cell growth in the leaf blade epidermis. Low nitrogen supply reduced leaf elongation rate (LER) by 43% through a similar decrease in the cell production rate and final cell length. The former was entirely because of a decreased average cell division rate (0.023 versus 0.032 h(-1)) and thus longer cell cycle duration (30 versus 22 h). Nitrogen status did not affect the number of division cycles of the initial cell's progeny (5.7), and accordingly the meristematic cell number (53). Meristematic cell length was unaffected by nitrogen deficiency, implying that the division and mitotic growth rates were equally impaired. The shorter mature cell length arose from a considerably reduced post-mitotic growth rate (0.033 versus 0.049 h(-1)). But, nitrogen stress did not affect the position where elongation stopped, and increased cell elongation duration. In conclusion, nitrogen deficiency limited leaf growth by increasing the cell cycle duration and decreasing mitotic and post-mitotic elongation rates, delaying cell maturation.

  17. Phytoextraction of metals and rhizoremediation of PAHs in co-contaminated soil by co-planting of Sedum alfredii with ryegrass (Lolium perenne) or castor (Ricinus communis).

    Science.gov (United States)

    Wang, Kai; Huang, Huagang; Zhu, Zhiqiang; Li, Tingqiang; He, Zhenli; Yang, Xiaoe; Alva, Ashok

    2013-01-01

    A pot experiment was conducted to investigate the potential for phytoextraction of heavy metals and rhizoremediation of polycyclic aromatic hydrocarbons (PAHs) in co-contaminated soil by co-planting a cadmium/zinc (Cd/Zn) hyperaccumulator and lead (Pb) accumulator Sedum alfredii with ryegrass (Lolium perenne) or castor (Ricinus communis). Co-planting with castor decreased the shoot biomass of S. alfredii as compared to that in monoculture. Cadmium concentration in S. alfredii shoot significantly decreased when grown with ryegrass or castor as compared to that in monoculture. However, no reduction of Zn or Pb concentration in S. alfredii shoot was detected in co-planting treatments. Total removal of either Cd, Zn, or Pb by plants was similar across S. alfredii monoculture or co-planting with ryegrass or castor, except enhanced Pb removal in S. alfredii and ryegrass co-planting treatment. Co-planting of S. alfredii with ryegrass or castor significantly enhanced the pyrene and anthracene dissipation as compared to that in the bare soil or S. alfredii monoculture. This appears to be due to the increased soil microbial population and activities in both co-planting treatments. Co-planting of S. alfredii with ryegrass or castor provides a promising strategy to mitigate both metal and PAH contaminants from co-contaminated soils.

  18. Evaluating the suitability of maggot meal as a partial substitute of soya bean on the productive traits, digestibility indices and organoleptic properties of broiler meat.

    Science.gov (United States)

    Khan, S; Khan, R U; Sultan, A; Khan, M; Hayat, S U; Shahid, M S

    2016-08-01

    This study was conducted to replace soya bean meal with maggot meal at difference percentages. A total of 120 one-day-old broiler chicks were divided into four groups. Four diets were formulated with maggot meal to replace soya bean meal at the rate of 0%, 10%, 20% and 30% designated as A, B, C and D respectively. Feed and water were offered ad libitum during the study which lasted for 28 days. Feed intake decreased significantly in treated groups while body weight, Feed conversion ratio and dressing percentage increased significantly in group D. Apparent metabolizable energy increased significantly in the group fed 30% maggot meal; however, no significant difference was found in dry matter, crude protein, crude fibre, ether extract, ash and nitrogen free extract between the control and treated groups. The result of sensory evaluation revealed that tenderness and juiciness increased significantly in group C and B respectively. The colour and flavour were most accepted in group B and C. The results indicate that replacement of soya bean meal at the rate of 30% in broiler feed produced most favourable results. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

  19. First performance assessment of blends of jatropha, palm oil and soya bean biodiesel with kerosene as fuel for domestic purposes in rural-Ghana

    Energy Technology Data Exchange (ETDEWEB)

    Quansah, E.; Preko, K.; Amekudzi, L.K. [Department of Physics, Kwame Nkrumah, University of Science and Technology (KNUST), University Post Office, PMB Kumasi (Ghana)

    2011-07-01

    Performance assessments of jatropha, palm oil and soya bean based biodiesel were carried out to investigate their potential use as conventional substitute for kerosene for domestic purposes in rural- Ghana. The assessments were done by comparing some of the combustion characteristics of blends of the biodiesel with kerosene. The blends were categorised as B100 (100% biodiesel), B80 (80% biodiesel and 20% kerosene), B60 (60% biodiesel and 40% kerosene), B40 (40% biodiesel and 60% kerosene), B20 (20% biodiesel and 80% kerosene) and B0 (pure kerosene). The results showed that the calorific values of the B100s were less than that of the B0 and decreasing in the order of jatropha, soya bean and palm oil. The wick wastage results for both the B100s and B0, revealed higher rates in the WTL than the BB even though the BB recorded low fuel consumption rates than the WTL for both B100s and B0. Similarly, the luminous intensity test with the B100s showed low values in WTL than the BB in a decreasing order of jatropha, soya bean and palm oil. However, B0 recorded higher luminous intensity values that were quite comparable in both WTL and BB.

  20. Relación entre Incidencia y Severidad de la Roya Asiática de la Soya Causada por Phakopsora pachyrhizi Sydow & Sydow Relationship Between Incidence and Severity of Asian Soybean Rust Caused by Phakopsora pachyrhizi Sydow & Sydow

    Directory of Open Access Journals (Sweden)

    Felipe Rafael Garcés Fiallos

    2011-12-01

    Full Text Available Se relaciona la incidencia con la severidad de la roya asiática de la soya, donde los valores generados por las ecuaciones pueden ser usados en cualquier estudio de este patosistema, proporcionando una herramienta para evaluar la incidencia como también la severidad. Se generaron gradientes de la enfermedad mediante el uso de fungicidas y momentos de aplicación, de manera preventiva y curativa. El experimento de campo con el cultivar Nidera 5909 RG, fue distribuido en bloques completos al azar. La intensidad de la roya asiática fue medida en los estratos inferior, medio y superior de la planta, a través de diferentes conteos de incidencia (porcentaje y severidad (lesiones cm-2, urédias cm-2 y porcentaje en folíolos. Las regresiones realizadas, presentaron una correlación significativa entre incidencia y severidad a 1% de probabilidad. Con los datos obtenidos, el evaluador puede escoger el método de cuantificación mas razonable o confiable a su criterio para otros trabajos de investigación o aplicar un determinado control de la enfermedad, utilizando las ecuaciones lineales generadas, facilitando el trabajo de cuantificar la roya asiática, bien se busque medir la incidencia (% o el número de lesiones cm-2, urédias cm-2 o severidad (%.Incidence is related to severity of the Asian soybean rust, where values generated by the equations can be used in any study of this pathosystem, providing a tool to evaluate incidence as well severity. Gradients of the disease were generated by means of the use of fungicides and moments of application, in a preventive and curative way. The field experiment with the cultivar Nidera RG 5909, was distributed in a randomized complete block. The intensity of Asian rust layers was measured in the lower, middle and top of the plant, through various counts of incidence (percentage and severity (lesions cm-2, uredinia cm-2 and percentage in leaflets. The regressions showed a significant correlation between

  1. Accurate determination of selected pesticides in soya beans by liquid chromatography coupled to isotope dilution mass spectrometry.

    Science.gov (United States)

    Huertas Pérez, J F; Sejerøe-Olsen, B; Fernández Alba, A R; Schimmel, H; Dabrio, M

    2015-05-01

    A sensitive, accurate and simple liquid chromatography coupled with mass spectrometry method for the determination of 10 selected pesticides in soya beans has been developed and validated. The method is intended for use during the characterization of selected pesticides in a reference material. In this process, high accuracy and appropriate uncertainty levels associated to the analytical measurements are of utmost importance. The analytical procedure is based on sample extraction by the use of a modified QuEChERS (quick, easy, cheap, effective, rugged, safe) extraction and subsequent clean-up of the extract with C18, PSA and Florisil. Analytes were separated on a C18 column using gradient elution with water-methanol/2.5 mM ammonium acetate mobile phase, and finally identified and quantified by triple quadrupole mass spectrometry in the multiple reaction monitoring mode (MRM). Reliable and accurate quantification of the analytes was achieved by means of stable isotope-labelled analogues employed as internal standards (IS) and calibration with pure substance solutions containing both, the isotopically labelled and native compounds. Exceptions were made for thiodicarb and malaoxon where the isotopically labelled congeners were not commercially available at the time of analysis. For the quantification of those compounds methomyl-(13)C2(15)N and malathion-D10 were used respectively. The method was validated according to the general principles covered by DG SANCO guidelines. However, validation criteria were set more stringently. Mean recoveries were in the range of 86-103% with RSDs lower than 8.1%. Repeatability and intermediate precision were in the range of 3.9-7.6% and 1.9-8.7% respectively. LODs were theoretically estimated and experimentally confirmed to be in the range 0.001-0.005 mg kg(-1) in the matrix, while LOQs established as the lowest spiking mass fractionation level were in the range 0.01-0.05 mg kg(-1). The method reliably identifies and quantifies the

  2. Videojuego con Realidad Virtual

    OpenAIRE

    González Mora, César

    2017-01-01

    El objetivo del proyecto es el desarrollo de un videojuego deportivo que utilice realidad mixta. El videojuego se podrá utilizar con dispositivos de tipo cardboard, y utilizará realidad aumentada para la interacción del jugador con el videojuego. En el desarrollo se utilizará el motor Unity para conseguir una aplicación multiplataforma, y la librería Vuforia para implementar realidad mixta.

  3. Sistemas integrados con Arduino

    OpenAIRE

    EL YAKOUTI, MOHAMMED

    2017-01-01

    Design of a robot prototype remotely controllable from Bluetooth using Arduino. Control and testing of sensors and events interacting with Arduino and Bluetooth. Diseño de un prototipo de robot controlable remotamente con Bluetooth utilizando Arduino. Control y verificación de los sensores y eventos que interactúan mediante el Arduino y el Bluetooth. El Yakouti, M. (2017). Sistemas integrados con Arduino. http://hdl.handle.net/10251/89274. TFGM

  4. Investigando con personas con dificultades de aprendizaje

    Directory of Open Access Journals (Sweden)

    Borja González Luna

    2013-12-01

    Full Text Available El artículo muestra los orígenes de lo que Walmsley (2008 denomina «investigación inclusiva». Para comprender qué se entiende por investigación inclusiva tenemos que remontarnos a los debates epistemológicos sobre las metodologías cuantitativas y cualitativas, acontecidos en la década de los 90, en torno a la revista Disability & Society. A partir de una síntesis de dichos debates, focalizados en el ámbito de la «discapacidad intelectual y del desarrollo», se exponen dos estrategias de colaboración con dicha población: a una aproximación etnográfica (de trabajo grupal, y b una aproximación biográfica (de trabajo individual. A continuación se esboza un posible diseño de trabajo de campo que intenta superar el paradigma cualitativo «clásico» con el objetivo de incluir a dicho colectivo más allá del rol de «sujetos de la investigación». Para finalizar se recoge el debate sobre la accesibilidad de los resultados de la investigación a los participantes en dichas investigaciones, y con ello la necesaria innovación en el ámbito de las «devoluciones» de los resultados, cuando se trata de incluir a personas que presentan limitaciones para la comprensión del lenguaje abstracto oral y/o escrito.

  5. The Effects of the Types of Milk (Cow, Goat, Soya and Enzymes (Rennet, Papain, Bromelain Toward Cheddar Cheese Production

    Directory of Open Access Journals (Sweden)

    Ariestya Arlene

    2015-04-01

    Full Text Available The objectives of this research are to study the effects of different types of milk and enzymes toward the yield and quality (moisture, ash, protein, fat content, and texture of cheddar cheese and the interaction between those two variables during the process. The types of milk are cow, goat, and soya milk, while the types of enzymes are rennet, papain, and bromelain enzymes. Regarding the procedure, the milk is first pasteurized before CaCl2 and Lactobacillus lactis that acts as the acidifier starter as much as 0.2% (w/v and 0.5% of the milk volume are added respectively. The amount of enzyme added is appropriate for the determination of enzyme dose. The curd is separated from the whey and then 2.5 grams of salt is added to 100 grams of curd. Afterwards, the curd is pressed until the water content decreases (cheese, then ripened for 1 month. The analyses conducted are moisture, ash, protein, fat content, and texture (hardness. The conclusion is the goat milk and the rennet enzyme are the suitable raw material for cheddar cheese production. Furthermore, different types of milk and enzymes affect the yield. However, there is no interaction between the types of milk and enzymes to the yield.

  6. Green synthesis of soya bean sprouts-mediated superparamagnetic Fe{sub 3}O{sub 4} nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Cai Yan [School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039 (China); Shen Yuhua, E-mail: s_yuhua@163.co [School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039 (China) and State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093 (China); Xie Anjian, E-mail: anjx@163.co [School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039 (China) and State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093 (China); Li Shikuo; Wang Xiufang [School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039 (China)

    2010-10-15

    Superparamagnetic Fe{sub 3}O{sub 4} nanoparticles were first synthesized via soya bean sprouts (SBS) templates under ambient temperature and normal atmosphere. The reaction process was simple, eco-friendly, and convenient to handle. The morphology and crystalline phase of the nanoparticles were determined from scanning electron microscopy (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), and X-ray diffraction (XRD) spectra. The effect of SBS template on the formation of Fe{sub 3}O{sub 4} nanoparticles was investigated using X-ray photoemission spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FT-IR). The results indicate that spherical Fe{sub 3}O{sub 4} nanoparticles with an average diameter of 8 nm simultaneously formed on the epidermal surface and the interior stem wall of SBS. The SBS are responsible for size and morphology control during the whole formation of Fe{sub 3}O{sub 4} nanoparticles. In addition, the superconducting quantum interference device (SQUID) results indicate the products are superparamagnetic at room temperature, with blocking temperature (T{sub B}) of 150 K and saturation magnetization of 37.1 emu/g.

  7. Standardized ileal digestibility of proteins and amino acids in sesame expeller and soya bean meal in weaning piglets.

    Science.gov (United States)

    Aguilera, A; Reis de Souza, T C; Mariscal-Landín, G; Escobar, K; Montaño, S; Bernal, M G

    2015-08-01

    Apparent ileal digestibility (AID) of diets containing sesame expeller (SE) and soya bean meal (SBM) was determined using 15 piglets (Genetiporc(®)), weaned at 17 ± 0.4 days with average body weight of 6.4 ± 0.7 kg (Fertilis 20 × G Performance, Genetiporc(®), PIC México, Querétaro, México). Piglets were randomly assigned to three treatments: (i) a reference diet with casein as the sole protein source; (ii) a mixed diet of casein-SE; and (iii) a mixed diet of casein-SBM. The chemical composition of SE and SBM was determined, and AID and standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AAs) were determined for each protein source. SE contained greater quantities of ether extract, neutral detergent fibre, phytic acid, methionine and arginine than SBM. Lysine and proline contents and trypsin inhibitor activity were higher in SBM than in SE. The AID and SID of CP and AA (except for lysine and proline) were similar in SE and SBM. The AID of lysine and proline was higher in SBM than in SE (p < 0.05), and the SID of proline was higher in SE than in SBM (p < 0.05). These findings indicate that SE is an appropriate alternative protein source for early weaned pigs. Journal of Animal Physiology and Animal Nutrition © 2014 Blackwell Verlag GmbH.

  8. Testing of Two Varieties of Lupin Seeds as Substitutes for Soya Extracted Meal in Vegetable Diets Designed for Young Broilers

    Directory of Open Access Journals (Sweden)

    P. Suchý

    2006-01-01

    Full Text Available The main aim of this experiment was to verify the suitability of two Polish varieties of dehulled lupin seed (the low-protein SONET variety and the high-protein JUNO variety as protein components to replace 50% of soya extract meal in feeding mixtures designed for the feeding of young broilers. The results of this experiment demonstrate that suitable treatment of lupin seed (dehulling may significantly increase its nutritional value. Dehulling provides a product (core in which the level of nitrogen-containing substances is by 27% higher than that in the original seed, providing an important protein component to be added in feeding mixtures for chickens. The experiment has also shown that production performance depends on the particular Lupinus variety used. Chickens fed on the JUNO variety seed (Group E1 reached a live weight of 2.332 kg on Day 40, which is comparable with the control group of chickens (2.337 kg; Group C. However, chickens in Group E2 fed on the SONET variety seed had a conclusively lower average weight (2.280 kg (P Lupinus genus is a major source of the amino acid arginine, which is often deficient in the feeding mixtures.

  9. Use of γ-irradiation to produce films from whey, casein and soya proteins: structure and functionals characteristics

    International Nuclear Information System (INIS)

    Lacroix, M.; Le, T.C.; Ouattara, B.; Yu, H.; Letendre, M.; Sabato, S.F.; Mateescu, M.A.; Patterson, G.

    2002-01-01

    γ-irradiation and thermal treatments have been used to produce sterilized cross-linked films. Formulations containing variable concentrations of calcium caseinate and whey proteins (whey protein isolate (WPI) and commercial whey protein concentrate) or mixture of soya protein isolate (SPI) with WPI was investigated on the physico-chemical properties of these films. Results showed that the mechanical properties of cross-linked films improved significantly the puncture strength for all types of films. Size-exclusion chromatography showed for no cross-linked proteins, a molecular mass of around 40 kDa. The soluble fractions of the cross-linked proteins molecular distributions were between 600 and 3800 kDa. γ-irradiation seems to modify to a certain extent the conformation of proteins which will adopt structures more ordered and more stable, as suggested by X-ray diffraction analysis. Microstructure observations showed that the mechanical characteristics of these films are closely related to their microscopic structure. Water vapor permeability of films based on SPI was also significantly decreased when irradiated. Microbial resistance was also evaluated for cross-linked films. Results showed that the level of biodegradation of cross-linked films was 36% after 60 d of fermentation in the presence of Pseudomonas aeruginosa

  10. Use of {gamma}-irradiation to produce films from whey, casein and soya proteins: structure and functionals characteristics

    Energy Technology Data Exchange (ETDEWEB)

    Lacroix, M. E-mail: monique.lacroix@inrs-iaf.uquebec.ca; Le, T.C.; Ouattara, B.; Yu, H.; Letendre, M.; Sabato, S.F.; Mateescu, M.A.; Patterson, G

    2002-03-01

    {gamma}-irradiation and thermal treatments have been used to produce sterilized cross-linked films. Formulations containing variable concentrations of calcium caseinate and whey proteins (whey protein isolate (WPI) and commercial whey protein concentrate) or mixture of soya protein isolate (SPI) with WPI was investigated on the physico-chemical properties of these films. Results showed that the mechanical properties of cross-linked films improved significantly the puncture strength for all types of films. Size-exclusion chromatography showed for no cross-linked proteins, a molecular mass of around 40 kDa. The soluble fractions of the cross-linked proteins molecular distributions were between 600 and 3800 kDa. {gamma}-irradiation seems to modify to a certain extent the conformation of proteins which will adopt structures more ordered and more stable, as suggested by X-ray diffraction analysis. Microstructure observations showed that the mechanical characteristics of these films are closely related to their microscopic structure. Water vapor permeability of films based on SPI was also significantly decreased when irradiated. Microbial resistance was also evaluated for cross-linked films. Results showed that the level of biodegradation of cross-linked films was 36% after 60 d of fermentation in the presence of Pseudomonas aeruginosa.

  11. Development by extrusion of soyabari snack sticks: a nutritionally improved soya-maize product based on the Nigerian snack (kokoro).

    Science.gov (United States)

    Omueti, O; Morton, I D

    1996-01-01

    A nutritionally improved local snack compared to existing kokoro has been developed by extrusion cooking of different formulations of maize, soybean and condiments such as pepper, onion, salt, palm oil, plantain and banana. The improved snack was named as the 'soyabari snack stick'. The chemical composition of representative extruded products indicates a high level of crude protein, fat, energy, available lysine and improved in vitro digestibility compared to the usual maize-based products. The level of stachyose and raffinose were greatly reduced in the extruded products compared to raw soya. Formulations using various additives yielded products suitable for different consumers' preferences such as hot, sweet, bland, gritty or crispy and acceptable to taste assessors. Soyabari snack sticks were equally acceptable as Bombay mix, a product on the market in London. Sensory analysis showed no significant differences in the two products but the crude fibre content of Bombay mix was higher while the protein was slightly lower than for soyabari sticks. Local ingredients can produce acceptable extrudates.

  12. PESO DE HOJAS COMO HERRAMIENTA PARA ESTIMAR EL ÁREA FOLIAR EN SOYA

    Directory of Open Access Journals (Sweden)

    Felipe Rafael Garcés Fiallos

    2011-07-01

    Full Text Available Cuantificaciones del área foliar en plantas son importantes en estudios de daños ocasionados por enfermedades, por lo tanto su determinación requiere el uso de equipamientos que no siempre se encuentran disponibles para todos. La utilización de determinaciones indirectas, como el peso de materia fresca o seca podría ayudar en este proceso. En este trabajo, se evaluó la relación entre el peso de hojas y área foliar, a partir de plantas recolectadas en el estadío R7.1, en 64 parcelas de campo con el cultivar de soja Nidera 5909 RG. El peso fresco fue medido luego de la colecta, el peso seco después de 48 horas de incubación a 65° C y el área foliar a través de un integralizador digital Licor. Fueron obtenidas ecuaciones significativas (p < 0.0001 e R2 de 0.74 a 0.97 para cada estrato y para la planta entera. Para la media de la planta, la relación de área foliar fue de y = 45.53 x + 19.03 para peso fresco e y = 176.17 x – 75.30 para peso seco. Esta herramienta se presenta potencialmente viable para estimar el área foliar de la planta. La utilización del peso seco es mas trabajosa, más no requiere pesaje de las hojas inmediatamente después de su colecta. La utilización futura de esta herramienta requiere estudios adicionales con otros cultivares a fin de verificarse si el comportamiento es similar.

  13. Lambs Fed Fresh Winter Forage Rape (Brassica napus L.) Emit Less Methane than Those Fed Perennial Ryegrass (Lolium perenne L.), and Possible Mechanisms behind the Difference

    Science.gov (United States)

    Sun, Xuezhao; Henderson, Gemma; Cox, Faith; Molano, German; Harrison, Scott J.; Luo, Dongwen; Janssen, Peter H.; Pacheco, David

    2015-01-01

    The objectives of this study were to examine long-term effects of feeding forage rape (Brassica napus L.) on methane yields (g methane per kg of feed dry matter intake), and to propose mechanisms that may be responsible for lower emissions from lambs fed forage rape compared to perennial ryegrass (Lolium perenne L.). The lambs were fed fresh winter forage rape or ryegrass as their sole diet for 15 weeks. Methane yields were measured using open circuit respiration chambers, and were 22-30% smaller from forage rape than from ryegrass (averages of 13.6 g versus 19.5 g after 7 weeks, and 17.8 g versus 22.9 g after 15 weeks). The difference therefore persisted consistently for at least 3 months. The smaller methane yields from forage rape were not related to nitrate or sulfate in the feed, which might act as alternative electron acceptors, or to the levels of the potential inhibitors glucosinolates and S-methyl L-cysteine sulfoxide. Ruminal microbial communities in forage rape-fed lambs were different from those in ryegrass-fed lambs, with greater proportions of potentially propionate-forming bacteria, and were consistent with less hydrogen and hence less methane being produced during fermentation. The molar proportions of ruminal acetate were smaller and those of propionate were greater in forage rape-fed lambs, consistent with the larger propionate-forming populations and less hydrogen production. Forage rape contained more readily fermentable carbohydrates and less structural carbohydrates than ryegrass, and was more rapidly degraded in the rumen, which might favour this fermentation profile. The ruminal pH was lower in forage rape-fed lambs, which might inhibit methanogenic activity, shifting the rumen fermentation to more propionate and less hydrogen and methane. The significance of these two mechanisms remains to be investigated. The results suggest that forage rape is a potential methane mitigation tool in pastoral-based sheep production systems. PMID:25803688

  14. Study of the epitope structure of purified Dac G I and Lol p I, the major allergens of Dactylis glomerata and Lolium perenne pollens, using monoclonal antibodies.

    Science.gov (United States)

    Mourad, W; Mécheri, S; Peltre, G; David, B; Hébert, J

    1988-11-15

    The use of mAb allowed us to further analyze the cross-reactivity between purified Dac g I and Lol p I, the major allergens of Dactylis glomerata (cocksfoot) and Lolium perenne (Rye grass), respectively. It was first shown, using IEF, followed by immunoprinting, that serum IgE antibodies from most grass-sensitive patients recognize both Dac g I and Lol p I. Second, three different anti-Lol p I mAb, 290A-167, 348A-6, and 539A-6, and one anti-Dac g I mAb, P3B2 were all shown to react with Dac g I and Lol p I, indicating that the two molecules share common epitopes. Epitope specificity of the mAb was determined by competitive binding inhibition of a given labeled mAb to solid phase fixed Dac g I or Lol p I by the mAb. The results indicated that the four mAb are directed against four different and non-overlapping epitopes present on both allergens. Using double-binding RIA, our data strongly suggest that the common epitopes are not repetitive on both molecules. In addition to their similar physicochemical characteristics, such as isolectric points and m.w., Dac g I and Lol p I share four identical epitopes. Binding inhibition of human IgE to Lol p I and Dac g I by the mAb was also assessed. The results indicated that each mAb was able to inhibit such reactions to variable degree but no additive inhibition was observed when two mAb of different specificities were used in combination, suggesting that the human IgE binding site is partially shared by each epitope recognized by the four mAb.

  15. Giochiamo con i robot

    Directory of Open Access Journals (Sweden)

    Andrea Bonarini

    2009-01-01

    Full Text Available "Giochiamo con i robot" e' un laboratorio interattivo per grandi e piccini realizzato per l'edizione 2007 del Festival della Scienza di Genova. Lungo un percorso che va dalla telerobotica alla robotica evolutiva, il laboratorio sviluppa il tema di dare intelligenza ai robot. Questo percorso, le cui tappe sono le varie installazioni, si conclude nella "bottega" dove e' possibile costruire e programmare i propri robot o smontare e modificare quelli esposti durante il percorso didattico. I visitatori sono coinvolti in attivita' ludiche grazie alle quali possonoentrare in contatto con alcune delle idee potenti della robotica,

  16. disegnare con ... Alberto Pratelli

    Directory of Open Access Journals (Sweden)

    Roberto Mingucci

    2017-12-01

    Full Text Available Con questa breve intervista ad Alberto Pratelli, (non a caso scelto per aprire questa nuova rubrica intendia-mo inaugurare un dialogo con personalità significati-ve del Disegno di Architettura, che consenta riflessioni dedicate alle sue varie dimensioni, oggi più che mai da approfondire. La suggestione a farlo, viene da un’idea di Pablo Rodri-guez Navarro ed abbiamo quindi pensato di avviarla proprio in questo numero, che Pablo ha accettato di curare su un tema a lui particolarmente caro.

  17. The Effect of using Quail Litter Biochar on Soybean (Glycine max L. Merr. Production Efecto del uso de Biocarbón de Lecho de Codorniz en la Producción de Soya (Glycine max L. Merr.

    Directory of Open Access Journals (Sweden)

    Tawadchai Suppadit

    2012-06-01

    Full Text Available Biochars can be used as soil amendments for improving soil properties and crop yield. The objective of this research was to study the plant growth, yield, yield components, and seed quality, including nutrients and heavy metals (Pb, Cd, and Hg, in the soybean plant (Glycine max L. Merr. and soil. The experiment was conducted from September 2010 to January 2011 in a greenhouse located in the Dan Khun Thot District, Nakhon Ratchasima Province, Thailand. The research comprised six treatments with four replicates in a completely randomized design. Quail litter biochar (QLB at rates of 0, 24.6, 49.2, 73.8, 98.4 and 123 g per pot mixture were provided to soybean cv. Chiang Mai 60. The results showed that QLB could be used as a soil fertility improvement and amendment for soybean production with an optimum rate of 98.4 g per pot mixture, which gave the best performance in terms of the number of nodes, height, DM accumulation, total yield, and seed quality. After the experiment, the nutrient contents in the soil increased as the QLB content increased, but the heavy metal residues in the leaves and seeds did not change. However, QLB at levels higher than 98.4 g per pot mixture is not advisable because QLB is alkaline in nature, which may affect soil pH.El biocarbón puede usarse como enmienda para mejorar las propiedades del suelo y el rendimiento del cultivo. El objetivo de esta investigación fue el estudio del crecimiento de la planta, rendimiento y sus características, así como la calidad de semilla, incluyéndose el estudio de nutrientes y metales pesados (Pb, Cd y Hg en la planta de soya (Glycine max L. Merr. y el suelo. La experimentación se realizó en condiciones de invernadero en el distrito de Dan Khun Thot, provincia de Nakhon Ratchasima, Tailandia, entre septiembre del 2010 y enero del 2011. La investigación constó de seis tratamientos con cuatro repeticiones en un diseño completamente al azar. Se administró biocarbón de lecho de

  18. Combination of soya pulp and Bacillus coagulans lilac-01 improves intestinal bile acid metabolism without impairing the effects of prebiotics in rats fed a cholic acid-supplemented diet.

    Science.gov (United States)

    Lee, Yeonmi; Yoshitsugu, Reika; Kikuchi, Keidai; Joe, Ga-Hyun; Tsuji, Misaki; Nose, Takuma; Shimizu, Hidehisa; Hara, Hiroshi; Minamida, Kimiko; Miwa, Kazunori; Ishizuka, Satoshi

    2016-08-01

    Intestinal bacteria are involved in bile acid (BA) deconjugation and/or dehydroxylation and are responsible for the production of secondary BA. However, an increase in the production of secondary BA modulates the intestinal microbiota due to the bactericidal effects and promotes cancer risk in the liver and colon. The ingestion of Bacillus coagulans improves constipation via the activation of bowel movement to promote defaecation in humans, which may alter BA metabolism in the intestinal contents. BA secretion is promoted with high-fat diet consumption, and the ratio of cholic acid (CA):chenodeoxycholic acid in primary BA increases with ageing. The dietary supplementation of CA mimics the BA environment in diet-induced obesity and ageing. We investigated whether B. coagulans lilac-01 and soya pulp influence both BA metabolism and the maintenance of host health in CA-supplemented diet-fed rats. In CA-fed rats, soya pulp significantly increased the production of secondary BA such as deoxycholic acid and ω-muricholic acids, and soya pulp ingestion alleviated problems related to plasma adiponectin and gut permeability in rats fed the CA diet. The combination of B. coagulans and soya pulp successfully suppressed the increased production of secondary BA in CA-fed rats compared with soya pulp itself, without impairing the beneficial effects of soya pulp ingestion. In conclusion, it is possible that a combination of prebiotics and probiotics can be used to avoid an unnecessary increase in the production of secondary BA in the large intestine without impairing the beneficial functions of prebiotics.

  19. Integrated Monitoring of the Soya Warm Current Using HF Ocean Radars, Satellite Altimeters, Coastal Tide Gauges, and a Bottom-Mounted ADCP

    Science.gov (United States)

    Ebuchi, N.; Fukamachi, Y.; Ohshima, K. I.; Wakatsuchi, M.

    2007-12-01

    The Soya Warm Current (SWC) is a coastal boundary current, which flows along the coast of Hokkaido in the Sea of Okhotsk. The SWC flows into the Sea of Okhotsk from the Sea of Japan through the Soya/La Perouse Strait, which is located between Hokkaido, Japan, and Sakhalin, Russia. It supplies warm, saline water in the Sea of Japan to the Sea of Okhotsk and largely affects the ocean circulation and water mass formation in the Sea of Okhotsk, and local climate, environment and fishery in the region. However, the SWC has never been continuously monitored due to the difficulties involved in field observations related to, for example, severe weather conditions in the winter, political issues at the border strait, and conflicts with fishing activities in the strait. Detailed features of the SWC and its variations have not yet been clarified. In order to monitor variations in the SWC, three HF ocean radar stations were installed around the strait. The radar covers a range of approximately 70 km from the coast. It is shown that the HF radars clearly capture seasonal and subinertial variations of the SWC. The velocity of the SWC reaches its maximum, approximately 1 m/s, in summer, and weakens in winter. The velocity core is located 20 to 30 km from the coast, and its width is approximately 50 km. The surface transport by the Soya Warm Current shows a significant correlation with the sea level difference along the strait, as derived from coastal tide gauge records. The cross-current sea level difference, which is estimated from the sea level anomalies observed by the Jason-1 altimeter and a coastal tide gauge, also exhibits variation in concert with the surface transport and along-current sea level difference.

  20. BASALIT trial: double-blind placebo-controlled allergen immunotherapy with rBet v 1-FV in birch-related soya allergy.

    Science.gov (United States)

    Treudler, R; Franke, A; Schmiedeknecht, A; Ballmer-Weber, B; Worm, M; Werfel, T; Jappe, U; Biedermann, T; Schmitt, J; Brehler, R; Kleinheinz, A; Kleine-Tebbe, J; Brüning, H; Ruëff, F; Ring, J; Saloga, J; Schäkel, K; Holzhauser, T; Vieths, S; Simon, J C

    2017-08-01

    Conflicting results exist on the effect of allergen immunotherapy (AIT) on pollen-related food allergy. We aimed to investigate the efficacy of one-year AIT with the folding variant (FV) of recombinant (r) Bet v 1 on birch-related soya allergy. Of 138 subjects with Bet v 1 sensitization, 82 were positive at double-blind placebo-controlled food challenge (DBPCFC) with soya. A total of 56 of 82 were randomized in the ratio of 2:1 (active: placebo). Per-protocol population (PPP) had received ≥150 μg of allergen or placebo preparation. lowest observed adverse effect levels (LOAEL), postinterventional occurrence of objective signs (objS) at any dose level, sIgE/IgG4 against Bet v 1 and Gly m 4. Between-group changes were investigated (ancova, Mann-Whitney U-test, Fisher exact test). Baseline characteristics including LOAELs were comparable in both groups with objS and subjS occurring in 82% and 95% of active (n = 38) vs 78% and 83% of placebo group (n = 18). After AIT, objS occurred in 24% and 47%, respectively. LOAEL group differences showed a beneficial tendency (P = 0.081) for LOAEL objective in PPP (30 active, 15 placebo). sIgG4 raised only in active group (Bet v 1: P = 0.054, Gly m 4: P = 0.037), and no relevant changes occurred for sIgE. Only 56% of the intended sample size was recruited. For the first time, we present data on the effect of rBet v 1-FV on birch-related soya allergy. rBet v 1-FV AIT induced significant immunogenic effects. Clinical assessment showed a tendency in favour of the active group but did not reach statistical significance. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  1. Cryopreservation of Dog Semen in a Tris Extender with 1% or 2% Soya Bean Lecithin as a Replacement of Egg Yolk.

    Science.gov (United States)

    Axnér, E; Lagerson, E

    2016-04-01

    Egg yolk is usually included in extenders used for preservation of dog semen. Lecithin is an interesting animal-protein free alternative to egg yolk for semen preservation. The aim of our study was to evaluate soya bean lecithin for cryopreservation of dog semen. Five ejaculate replicates were divided in three equal parts, centrifuged and each pellet diluted with one of the three Tris-based extenders containing 20% egg yolk, 1% soya bean lecithin or 2% soya bean lecithin. Extended semen was loaded in 0.5-ml straws, cooled and diluted a second time and frozen in liquid nitrogen vapours. Sperm motility parameters (CASA), acrosome integrity (FITC-PNA/PI) and sperm membrane integrity (C-FDA) were evaluated 5 min post-thaw and after 2 and 4 h of incubation. Total motility was significantly better in the egg yolk extender than in any of the lecithin-based extender and was better in the 1% lecithin extender than in the 2% lecithin extender. Sperm membrane integrity was significantly better in the egg yolk extender than in any of the lecithin-based extenders but did not differ significantly between the 1% and 2% lecithin extenders. Acrosome integrity was significantly better in the egg yolk extender than in the 2% lecithin extender but did not differ between the egg yolk extender and the 1% lecithin extender or between the two lecithin extenders. In conclusion, egg yolk was superior to lecithin in our study. The extender with 1% lecithin preserved sperm motility better than the extender with 2% lecithin. © 2016 Blackwell Verlag GmbH.

  2. Soil persistence, plant and non-target insect uptake of endosulfan and lindane applied to soya bean and maize in field trials in Zimbabwe

    International Nuclear Information System (INIS)

    Zaranyika, M.F.; Mugari, P.

    1997-01-01

    The persistence of lindane and endosulfan in the soil, uptake by, and distribution in plants, and effects on and absorption by non-target insects, following application of the insecticides for the control of maize pests and soya bean respectively were determined under Zimbabwean weather conditions. No large scale effects on the non-target insects were observed though some small effects on the populations of semiloopers and orthoptera in the endosulfan treated soya bean plot were noted. Concentrations of the insecticides in spiders declined during the trial though those in grasshoppers and crickets appeared to increase. Concentrations of both insecticides in soil fell rapidly during the first 7 weeks after application but, after that, the rates of loss were much slower possibly owing to the drier conditions prevailing during this later period, reducing both physicochemical and microbial loss processes. Initial concentrations of both insecticides in all the vegetative parts of plants examined after spray application declined systematically to low levels during the 10 weeks of observations, probably owing to both metabolism within the plants and to crop volume dilution effects and will have declined to even lower levels by harvest time. Surprisingly, low concentrations of lindane and endosulfan were found in the harvested maize and soya bean seeds. At early stages after application, there were also traces of both insecticides in the vegetative parts of the plants from the untreated, control plots probably arising from uptake of soil residues from the previous year and/or spray drift but these became undetectable at later stages of growth. (author). 31 refs, 7 tabs

  3. Conversando con Oriol Bohigas

    OpenAIRE

    Redondo Domínguez, Ernesto; Moya Sala, Joaquim

    2015-01-01

    [EN] Interview with Oriol Bohigas [ES] Entrevista con Oriol Bohigas Redondo Domínguez, E.; Moya Sala, J. (2015). Conversando con… Oriol Bohigas. EGA. Revista de Expresión Gráfica Arquitectónica. 20(26):22-35. doi:10.4995/ega.2015.4061 22 35 20 26

  4. DR Con o:

    African Journals Online (AJOL)

    which could fall under the Ugandan influence. The con-. flict in the ..... The Congolese people and international community within SADC, the AU ..... ments and make peace among themselves. However, one ... friends overnight.There is a great ...

  5. RESPUESTA PRODUCTIVA Y PERFIL LIPÍDICO DE LA LECHE DE CABRAS ALIMENTADAS EN CONFINAMIENTO ADICIONANDO ACEITE DE SOYA EN LA DIETA

    OpenAIRE

    MEJÍA URIBE, LUIS ALBERTO

    2016-01-01

    La leche de cabra y sus productos (queso, yogurt) son una importante fuente de nutrientes tales como lípidos, proteínas, vitaminas y minerales, que pueden ser considerados como parte de la dieta de los humanos, sobre todo para personas intolerantes a ciertos componentes (lactosa) de la leche de vaca. El objetivo de la presente investigación fue evaluar el efecto de la adición de aceite de soya (6% BS) sobre la producción, composición y perfil de ácidos grasos (AG) de la leche de cabras Saanen...

  6. fertilizada con diferentes abonos

    Directory of Open Access Journals (Sweden)

    Jorge Alberto Elizondo-Salazar

    2007-01-01

    Full Text Available Producción y calidad de la biomasa de morera (Morus alba fertilizada con diferentes abonos. Se llevó a cabo un experimento en la Estación Experimental “Alfredo Volio Mata” de la Universidad de Costa Rica con el fi n de evaluar la aplicación de 150 kg de N/ha/año proveniente de dos abonos orgánicos: lombriabono y compostaje; y de un fertilizante químico, sobre la producción y calidad de la biomasa de morera. El periodo experimental comprendió un ciclo de 12 meses, iniciando en julio del 2003 y fi nalizando en julio del 2004. Se utilizó una plantación de morera de 12 años de establecida con una densidad de siembra de 27.777 plantas/ ha. Se empleó un diseño de bloques completos al azar con cuatro tratamientos: dos abonos orgánicos, nitrato de amonio (33,5% N y un control. Las plantas se podaron a 0,6 m sobre el nivel del suelo al inicio del ensayo. Durante el periodo experimental, las plantas fueron podadas consecutivamente cada 90 días. Las hojas y los tallos fueron separados y analizados para determinar el contenido de materia seca y proteína cruda. La producción de materia seca fue 23% superior y el contenido de proteína cruda fue signifi cativamente mayor con el nitrógeno químico, mientras que el contenido de materia seca fue menor. No se encontraron diferencias signifi cativas entre el tratamiento control y los tratamientos orgánicos.

  7. T cell epitopes of the major fraction of rye grass Lolium perenne (Lol p I) defined using overlapping peptides in vitro and in vivo. I. Isoallergen clone1A.

    Science.gov (United States)

    Bungy Poor Fard, G A; Latchman, Y; Rodda, S; Geysen, M; Roitt, I; Brostoff, J

    1993-10-01

    One hundred and fifteen overlapping synthetic peptides spanning the entire sequence of the iso-allergen clone1A of Lol p I from rye grass Lolium perenne were synthesized by the multi-pin technique. The peptides were overlapping 12mers, offset by two residues and overlapping by 10 residues. Sets of six adjacent overlapping peptides (except pool-1, 15, 20) were pooled and were used in vitro and in vivo to map the T cell epitopes on Lol p I. Six atopics who were skin test and RAST positive to rye grass showed T cell responses to L. perenne extract (LPE) and its major fraction (Lol p I). Five out of six showed T cell responses in vitro to peptide pool-17, while five non-atopics did not respond to any of the peptide pools. By testing the individual peptides of pool-17, we have located the T cell epitope on Lol p I. Interestingly, when we tested pool-17 and its single peptides in vivo by intradermal skin testing we found in one patient a typical DTH after 24-48 h to pool-17 and its peptides (peptides 3 and 4) which exactly matched the in vitro responses. By defining the T cell epitopes in this way a greater understanding of the allergic response to pollen will be obtained, and a more effective and less dangerous vaccine may be possible for treating patients with hay fever.

  8. The combined effects of soya isoflavones and resistant starch on equol production and trabecular bone loss in ovariectomised mice.

    Science.gov (United States)

    Tousen, Yuko; Matsumoto, Yu; Matsumoto, Chiho; Nishide, Yoriko; Nagahata, Yuya; Kobayashi, Isao; Ishimi, Yoshiko

    2016-07-01

    Equol is a metabolite of the soya isoflavone (ISO) daidzein that is produced by intestinal microbiota. Equol has greater oestrogenic activity compared with other ISO, and it prevents bone loss in postmenopausal women. Resistant starch (RS), which has a prebiotic activity and is a dietary fibre, was reported to promote equol production. Conversely, the intestinal microbiota is reported to directly regulate bone health by reducing inflammatory cytokine levels and T-lymphocytes in bone. The present study evaluated the combined effects of diet supplemented with ISO and RS on intestinal microbiota, equol production, bone mineral density (BMD) and inflammatory gene expression in the bone marrow of ovariectomised (OVX) mice. Female ddY strain mice, aged 8 weeks, were either sham-operated (Sham, n 7) or OVX. OVX mice were randomly divided into the following four groups (seven per group): OVX control (OVX); OVX fed 0·05 % ISO diet (OVX+ISO); OVX fed 9 % RS diet (OVX+RS); and OVX fed 0·05 % ISO- and 9 % RS diet (OVX+ISO+RS). After 6 weeks, treatment with the combination of ISO and RS increased equol production, prevented the OVX-induced decline in trabecular BMD in the distal femur by modulating the enteric environment and altered OVX-induced inflammation-related gene expression in the bone marrow. However, there were no significant differences in bone parameters between the ISO+RS and ISO-alone groups in OVX mice. Our findings suggest that the combination of ISO and RS might alter intestinal microbiota and immune status in the bone marrow, resulting in attenuated bone resorption in OVX mice.

  9. Anthocyanin contents in the seed coat of black soya bean and their anti-human tyrosinase activity and antioxidative activity.

    Science.gov (United States)

    Jhan, J-K; Chung, Y-C; Chen, G-H; Chang, C-H; Lu, Y-C; Hsu, C-K

    2016-06-01

    The seed coat of black soya bean (SCBS) contains high amount of anthocyanins and shows antioxidant and anti-mushroom tyrosinase activities. The objectives of this study were to analyse the anthocyanins in SCBS with different solvents and to find the relationship between anthocyanin profile with anti-human and anti-mushroom tyrosinase activities. SCBS was extracted with hot water, 50 and 80% ethanol, 50 and 80% acetone and 50 and 80% acidified acetone. Total phenol and total flavonoid contents in the extracts were determined. Anthocyanins in the extracts were analysed using HPLC and LC/MS/MS. A genetically engineered human tyrosinase was used to evaluate the anti-tyrosinase potential of the extracts from SCBS. 80% acetone extract from SCBS obtained the highest total phenol, total flavonoid and cyanidin-3-O-glucoside (C3G) contents among all the extracts, whereas the hot water extract showed the lowest antioxidant contents. Three anthocyanin compounds were found in all the extracts from SCBS, and the analysis of HPLC and LC/MS/MS indicated that they were C3G, delphinidin-3-O-glucoside (D3G) and peonidin-3-O-glucoside (P3G). The ratios of C3G (2.84 mg g(-1) ), D3G (0.34 mg g(-1) ) and P3G (0.35 mg g(-1) ) in 80% acidified acetone extract were 76.6, 9.1 and 9.3%, respectively. All the extracts from SCBS possessed anti-human tyrosinase activity. Moreover, a good correlation was found between the anti-human tyrosinase activities and C3G contents in the extracts. Antioxidants in SCBS also possess anti-human and anti-mushroom tyrosinase activities. © 2015 Society of Cosmetic Scientists and the Société Française de Cosmétologie.

  10. Nonomuraea glycinis sp. nov., a novel actinomycete isolated from the root of black soya bean [Glycine max (L.) Merr].

    Science.gov (United States)

    Li, Zhilei; Song, Wei; Zhao, Junwei; Zhuang, Xiaoxin; Zhao, Yue; Wang, Xiangjing; Xiang, Wensheng

    2017-12-01

    A novel actinomycete, designated strain NEAU-BB2C19 T , was isolated from the root of black soya bean [Glycine max (L.) Merr] collected from Harbin, Heilongjiang Province, China, and characterized using a polyphasic approach. The strain was an aerobic, Gram-stain-positive actinomycete that formed extensively branched substrate mycelium and aerial hyphae. The predominant menaquinones were MK-9(H2) and MK-9(H0). The major cellular fatty acid profile consisted of iso-C16 : 0, 10-methyl C17 : 0 and 10-methyl C18 : 0. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylglycerol and glycolipid. The DNA G+C content was 68.2±0.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-BB2C19 T should be assigned to the genus Nonomuraea and formed a distinct branch with its closest neighbour Nonomuraea guangzhouensis NEAU-ZJ3 T (98.75 % 16S rRNA gene sequence similarity). The morphological and chemotaxonomic properties of the strain were also consistent with those of members of the genus Nonomuraea. A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-BB2C19 T could be clearly differentiated from its closest phylogenetic relative. Thus, the strain is concluded to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea glycinis sp. nov. is proposed. The type strain is NEAU-BB2C19 T (=CGMCC 4.7430 T =DSM 104838 T ).

  11. Pyritic waste from precombustion coal cleaning: Amelioration with oil shale retort waste and sewage sludge for growth of soya beans

    International Nuclear Information System (INIS)

    Lewis, B.G.; Gnanapragasam, N.; Stevens, M.L.

    1994-01-01

    Solid residue from fossil fuel mining and utilization generally present little hazard to human health. However, because of the high volumes generated, they do pose unique disposal problems in terms of land use and potential degradation of soil and water. In the specific case of wastes from precombustion coal cleaning, the materials include sulfur compounds that undergo oxidation when exposed to normal atmospheric conditions and microbial action and then produce sulfuric acid. The wastes also contain compounds of metals and nonmetals at concentrations many times those present in the original raw coal. Additionally, the residues often contain coal particles and fragments that combust spontaneously if left exposed to the air, thus contributing to the air pollution that the coal cleaning process was designed to prevent. Federal and state efforts in the United States to ameliorate the thousands of hectares covered with these wastes have focused on neutralizing the acidity with limestone and covering the material with soil. The latter procedure creates additional degraded areas, which were originally farmland or wildlife habitat. It would seem preferable to reclaim the coal refuse areas without earth moving. The authors describe here experiments with neutralization of coal waste acidity using an alkaline waste derived from the extraction of oil from oil shale to grow soya beans (Glycine max. [L]) on a mixture of wastes and sewage sludge. Yield of plant material and content of nutrients an potentially toxic elements in the vegetation and in the growth mixtures were determined; results were compared with those for plants grown on an agricultural soil, with particular focus on boron

  12. Effects of substituting soya bean meal (SBM) with blood meal (BM) on biochemical profile of pregnant pigs.

    Science.gov (United States)

    Abonyi, Festus Otaka; Machebe, Ndubuisi Samuel; Ezea, Michael Sunday; Eze, James I; Omeke, Benjamin Chigozie; Marire, Benjamin Nwabueze

    2013-04-01

    Twenty-four Large White × Landrace crossbreed primigravid pigs, aged 7.50 to 8.00 months weighing between 86.15 and 88.24 kg were used to study the effects of feeding graded levels of soya bean meal (SBM) replaced blood meal (BM) diets on serum biochemical profile in gestating pigs. The pigs were randomly allotted to four finisher diets formulated such that BM replaced SBM at 0.0, 50.0, 75.0 and 100.0 %, respectively. The diets were T1 (100.0 % SBM, 0.0 % BM), T2 (50.0 % SBM, 50.0 % BM), T3 (25.0 % SBM, 75.0 % BM) and T4 (0.0 % SBM, 100.0 % BM). Individual animal's daily ration of the test diets was 2.20, 2.00 and 2.50 kg at stages one, two and three of gestation. Blood sampling and analysis for the effects of the test diets on biochemical profile of the experimental animals were carried out prior to conception, at weeks 3, 7 and 11 of gestation, respectively. The result showed no significant (P ≥ 0.05) dietary treatment effects on total protein, albumin, globulin fraction, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine and urea profile of the pigs fed with BM diets when compared to the control fed with 100.0 % SBM. There was however a significant (P ≤ 0.05) variation in these biochemical indices in all the experimental groups at different stages of gestation. It was concluded that BM can replace 100.0 % of SBM in the diets of pregnant pigs in the tropical humid environment without any deleterious effect on their health.

  13. Evaluation of stable isotope labelling strategies for the quantitation of CP4 EPSPS in genetically modified soya

    Energy Technology Data Exchange (ETDEWEB)

    Ocana, Mireia Fernandez [Centre for Chemical and Bioanalytical Sciences, Royal Holloway, University of London, Egham TW20 0EX (United Kingdom)], E-mail: Mireia.FernandezOcana@pfizer.com; Fraser, Paul D. [Centre for Chemical and Bioanalytical Sciences, Royal Holloway, University of London, Egham TW20 0EX (United Kingdom); Patel, Raj K.P.; Halket, John M. [Specialist Bioanalytical Services Ltd., Royal Holloway, University of London, Egham TW20 0EX (United Kingdom); Bramley, Peter M. [Centre for Chemical and Bioanalytical Sciences, Royal Holloway, University of London, Egham TW20 0EX (United Kingdom)

    2009-02-16

    The introduction of genetically modified (GM) crops into the market has raised a general alertness relating to the control and safety of foods. The applicability of protein separation hyphenated to mass spectrometry to identify the bacterial enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein expressed in GM crops has been previously reported [M.F. Ocana, P.D. Fraser, R.K.P. Patel, J.M. Halket, P.M. Bramley, Rapid Commun. Mass Spectrom. 21 (2007) 319.]. Herein, we investigate the suitability of two strategies that employ heavy stable isotopes, i.e. AQUA and iTRAQ, to quantify different levels of CP4 EPSPS in up to four GM preparations. Both quantification strategies showed potential to determine whether the presence of GM material is above the limits established by the European Union. The AQUA quantification procedure involved protein solubilisation/fractionation and subsequent separation using SDS-PAGE. A segment of the gel in which the protein of interest was located was excised, the stable isotope labeled peptide added at a known concentration and proteolytic digestion initiated. Following recovery of the peptides, on-line separation and detection using LC-MS was carried out. A similar approach was used for the iTRAQ workflow with the exception that proteins were digested in solution and generated tryptic peptides were chemically tagged. Both procedures demonstrated the potential for quantitative detection at 0.5% (w/w) GM soya which is a level below the current European Union's threshold for food-labelling. In this context, a comparison between the two procedures is provided within the present study.

  14. In vitro effects of inulin and soya bean oligosaccharide on skatole production and the intestinal microbiota in broilers.

    Science.gov (United States)

    Liu, H Y; Hou, R; Yang, G Q; Zhao, F; Dong, W G

    2018-06-01

    The experiment was conducted to investigate the in vitro effects of inulin and soya bean oligosaccharide (SBO) on the metabolism of L-tryptophan (L-try) to skatole production, and the intestinal microbiota in broilers. Treatments were as follows: caecal microbiota control (Cc), Cc + inulin, Cc + SBO, rectal microbiota control (Rc), Rc + inulin and Rc + SBO. Microbial suspensions were anaerobically incubated at 38°C for 24 hr. The results showed that concentrations of skatole and acetic acid were significantly lower in caecal microbiota fermentation broth (MFB) than those in rectal MFB (p inulin or SBO significantly decreased the concentrations of indole and skatole and rate of L-try degradation (p Inulin groups had lower indole than SBO groups (p inulin or SBO decreased the microbiota richness (p  .05). Four distinct bands were detected in inulin and SBO groups, which were related to two of Bacteroides, one of Firmicutes and Bifidobacteria. Six bands were detected only in control groups, which represented uncultured Rikenellaceae, Roseburia, Escherichia/Shigella dysenteriae, Bacteroides uniformis (T), Parabacteroides distasonis and Enterobacter aerogenes. Populations of Lactobacilli, Bifidobacteria and total bacteria in inulin groups were higher than those in control groups (p  .05). These results suggest that reduced concentrations of skatole and indole in the presence of inulin and SBO may be caused by decrease in L-try degradation rate, which were caused by change in microbial ecosystem and pH value. Uncultured B. uniformis (T) and E. aerogenes may be responsible for degradation of L-try to skatole. © 2017 Blackwell Verlag GmbH.

  15. Evaluation of stable isotope labelling strategies for the quantitation of CP4 EPSPS in genetically modified soya

    International Nuclear Information System (INIS)

    Ocana, Mireia Fernandez; Fraser, Paul D.; Patel, Raj K.P.; Halket, John M.; Bramley, Peter M.

    2009-01-01

    The introduction of genetically modified (GM) crops into the market has raised a general alertness relating to the control and safety of foods. The applicability of protein separation hyphenated to mass spectrometry to identify the bacterial enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein expressed in GM crops has been previously reported [M.F. Ocana, P.D. Fraser, R.K.P. Patel, J.M. Halket, P.M. Bramley, Rapid Commun. Mass Spectrom. 21 (2007) 319.]. Herein, we investigate the suitability of two strategies that employ heavy stable isotopes, i.e. AQUA and iTRAQ, to quantify different levels of CP4 EPSPS in up to four GM preparations. Both quantification strategies showed potential to determine whether the presence of GM material is above the limits established by the European Union. The AQUA quantification procedure involved protein solubilisation/fractionation and subsequent separation using SDS-PAGE. A segment of the gel in which the protein of interest was located was excised, the stable isotope labeled peptide added at a known concentration and proteolytic digestion initiated. Following recovery of the peptides, on-line separation and detection using LC-MS was carried out. A similar approach was used for the iTRAQ workflow with the exception that proteins were digested in solution and generated tryptic peptides were chemically tagged. Both procedures demonstrated the potential for quantitative detection at 0.5% (w/w) GM soya which is a level below the current European Union's threshold for food-labelling. In this context, a comparison between the two procedures is provided within the present study

  16. Evaluación de harina de yuca (20% en combinación con diferentes fuentes protéicas en dietas para pollos de engorde

    Directory of Open Access Journals (Sweden)

    Gómez de Martínez Carmen E.

    1989-06-01

    Full Text Available Se realizó un experimento para evaluar el efecto del nivel óptimo de inclusión (20% de harina de yuca en dietas para pollos de engorde en combinación con tres fuentes proteicas, sobre los rendimientos biológicos y sus resultados económicos. Las variables experimentales consistieron en 4 dietas (tratamientos que presentaron como fuente de variación los ingredientes proteicos; la dieta 10% de harina de yuca y tres fuentes de proteína (torta de soya, harina de pescado y torta de algodón, la dieta 2 con 20% de harina de yuca y las tres fuentes de proteína (torta de soya, harina de pescado y torta de algodón, la dieta 3 con 20% de harina de yuca y dos fuentes de proteína (torta de soya, harina de pescado y la dieta 4 con 20% de harina de yuca y una fuente de proteína (torta de soya. En el período de la 0-7 semanas, hubo comportamiento estadísticamente similar entre las dietas 1 y 2 (ganancia de peso: 1.679 vs 1.678 g; consumo de alimento: 3.521 vs 3A83 g; conversión: 2.09 vs 2.07. La dieta 3 aunque no presentó diferencias estadísticas con las anteriores, produjo menos ganancia de peso. La dieta 4 presentó. más bajas ganancias de peso (1.433 g y el menor consumo de alimento (2.972 g. El peso promedio final de los pollos fue de 1.986 g. (dieta 1, 1.991 (dieta 2, 1.994 (dieta 3 y 1.734 g. (dieta 4. El máximo beneficio neto se obtuvo con la dieta 2.An experiment was carried out to evaluate the effect of including the optimum level (20 % of cassava flour in diets for feed chicken in combination with three sources of protein, on the biological yields and their economic results. The experimental varieties were made up of 4 diets (treatments which varied in terms of the source protein: diet 1, 0% of cassava flour, and cottonseed meal, diet 2 with 20% cassava flour and three sources of protein (soybean meal, fish flour and cottonseed meal, diet 3 with 20% cassava flour and two sources of protein (soybean meal and fish flour, and diet 4

  17. pacientes con falla cardiaca

    Directory of Open Access Journals (Sweden)

    Diana Marcela Achury Saldaña

    2007-01-01

    Full Text Available Objetivo: determinar la adherencia al tratamiento de pacientes con falla cardiaca hospitalizados, al aplicar un plan educativo quefomenta el autocuidado.Método: estudio cuasiexperimental (entrevistas enfermera-paciente realizado entre diciembre de 2004 y mayo de 2006, con unamuestra de 50 pacientes seleccionados por conveniencia. Se diseñó un instrumento para evaluar los comportamientos de los pacientes,con base en algunos resultados de la adherencia y sus respectivos indicadores de la taxonomía NOC (Nursing out comes classification. Laadherencia al tratamiento fue medida en dos momentos: el primero durante la hospitalización, seguido de la aplicación del plan educativoantes del alta, que proporcionaba información en el manejo de su enfermedad desde una dimensión física, psicológica y social quepromueve el autocuidado; y el segundo un mes después del alta en su domicilio.Resultados: diferencias estadísticamente significativas (P=0,0001 que demuestran cómo mediante la capacitación al paciente enel manejo de su tratamiento farmacológico y no farmacológico, el establecimiento de una sana relación entre el profesional de enfermeríay el paciente, y la participación de la familia, se logra una total adherencia al tratamiento.Conclusiones: para lograr una adherencia total del paciente con falla cardiaca al tratamiento es necesario un proceso educativo y unseguimiento continuo y personalizado que motive permanentemente al paciente y se le reconozca el papel protagónico en su cuidado y manejo de la enfermedad.

  18. Process development for the removal and recovery of hazardous dye erythrosine from wastewater by waste materials-Bottom Ash and De-Oiled Soya as adsorbents

    Energy Technology Data Exchange (ETDEWEB)

    Mittal, Alok [Department of Applied Chemistry, Maulana Azad National Institute of Technology, Bhopal 462007 (India)]. E-mail: aljymittal@yahoo.co.in; Mittal, Jyoti [Department of Applied Chemistry, Maulana Azad National Institute of Technology, Bhopal 462007 (India); Kurup, Lisha [Department of Applied Chemistry, Maulana Azad National Institute of Technology, Bhopal 462007 (India); Singh, A.K. [Department of Applied Chemistry, University Institute of Technology, RGPV, Bhopal 462036 (India)

    2006-11-02

    Erythrosine is a water-soluble xanthene class of dye. It is widely used as colorant in foods, textiles, drugs and cosmetics. It is highly toxic, causes various types of allergies, thyroid activities, carcinogenicity, DNA damage behaviour, neurotoxicity and xenoestrogen nature in the humans and animals. The photochemical and biochemical degradation of the erythrosine is not recommended due to formation of toxic by-products. The present paper is an attempt to remove erythrosine from wastewater using adsorption over Bottom Ash-a power plant waste and De-Oiled Soya-an agricultural waste. Under the batch studies, effect of concentration of dye, temperature, pH of the solution, dosage of adsorbents, sieve size of adsorbents, etc., have been studied for the uptake of the dye over both adsorbents. The adsorption process verifies Langmuir and Freundlich adsorption isotherms in both the cases and based on the data different thermodynamic parameters have been evaluated. Batch studies also include kinetic measurements, rate constant study, mass transfer behaviour and establishment of mechanistic pathway for both the cases. For the bulk removal of the dye column operations have been carried out and breakthrough capacities of the Bottom Ash and De-Oiled Soya columns have been calculated. Attempts have also been made for the recovery of the adsorbed dye from exhausted columns by eluting dilute NaOH and more than 90% of the dye was recovered.

  19. A comparison of the European renewable energy directive default emission values with actual values from operating biodiesel facilities for sunflower, rape and soya oil seeds in Italy

    International Nuclear Information System (INIS)

    Buratti, C.; Barbanera, M.; Fantozzi, F.

    2012-01-01

    The European Union (EU) set a binding greenhouse gas (GHG) emission reduction target for transportation biofuels and other bioliquids. In this study, the GHG emissions of biodiesel chain from sunflower, rapeseed and soybean were calculated in compliance with the European Union Renewable Energy Directive 2009/28/EC (RED). Input data used for the agricultural step were referred to the Umbrian region for sunflower and rapeseed and to the Veneto region for soybean, while data obtained from the main Italian biodiesel plants were employed for the processing step. Results showed that GHG emissions were higher than default values reported in the RED for sunflower and rapeseed and lower for soybean. Only sunflower biodiesel does not reach the minimum value of GHG saving (35%). The main differences with data used in the RED concern cultivation step, while the processing step has overall the same values of GHG emissions. Finally, three case studies were examined in order to identify possible improvements to make the analyzed supply chains more sustainable. -- Highlights: ► GHG balance of biodiesel from sunflower, rapeseed and soya was analyzed. ► Cultivation has the highest environmental impact for sunflower and rapeseed chains. ► Typical Italian data were adopted for cultivation and processing steps. ► GHG emissions were lower than RED default values for soya biodiesel. ► Cogeneration for the processing plant can greatly increase GHG saving.

  20. New Polish Certified Reference Materials for inorganic trace analysis: corn flour (INCT-CF-3) and soya bean flour (INCT-SBF-4)

    International Nuclear Information System (INIS)

    Polkowska-Motrenko, H.; Dybczynski, R.S.; Chajduk, E.; Danko, B.; Kulisa, K.; Samczynski, Z.; Sypula, M.; Szopa, Z.

    2007-01-01

    Preparation and certification of two new Polish reference materials (CRMs) for inorganic trace analysis: Corn Flour (INCT-CF-3) and Soya Bean Flour (INCT-SBF-4) have been presented. The materials were prepared and certified in the Institute of Nuclear Chemistry and Technology, Warsaw (INCT). Instrumental neutron activation analysis (INAA) was applied to homogeneity and stability studies. Homogeneity tests have shown that both materials are homogeneous for the sample mass 3 100 mg. The results of trend analysis have shown that the materials are stable. Uncertainty caused by instability and, consequently, shelf life of the materials have been estimated. Certification was based on the statistical evaluation of results obtained in worldwide interlaboratory comparison, in which 92 laboratories from 19 countries participated. Results for CRM distributed by the organizer and analyzed together with the intercomparison samples as well as results by definitive methods for selected elements based on radiochemical neutron activation analysis (RNAA) were also employed in the certification process. The contents of 16 and 22 elements were certified in Corn Flour and Soya Bean Flour, respectively. Additional information values for 14 elements in INCT-CF-3 and 9 elements in INCT-SBF-4 were given. (authors)

  1. Process development for the removal and recovery of hazardous dye erythrosine from wastewater by waste materials-Bottom Ash and De-Oiled Soya as adsorbents

    International Nuclear Information System (INIS)

    Mittal, Alok; Mittal, Jyoti; Kurup, Lisha; Singh, A.K.

    2006-01-01

    Erythrosine is a water-soluble xanthene class of dye. It is widely used as colorant in foods, textiles, drugs and cosmetics. It is highly toxic, causes various types of allergies, thyroid activities, carcinogenicity, DNA damage behaviour, neurotoxicity and xenoestrogen nature in the humans and animals. The photochemical and biochemical degradation of the erythrosine is not recommended due to formation of toxic by-products. The present paper is an attempt to remove erythrosine from wastewater using adsorption over Bottom Ash-a power plant waste and De-Oiled Soya-an agricultural waste. Under the batch studies, effect of concentration of dye, temperature, pH of the solution, dosage of adsorbents, sieve size of adsorbents, etc., have been studied for the uptake of the dye over both adsorbents. The adsorption process verifies Langmuir and Freundlich adsorption isotherms in both the cases and based on the data different thermodynamic parameters have been evaluated. Batch studies also include kinetic measurements, rate constant study, mass transfer behaviour and establishment of mechanistic pathway for both the cases. For the bulk removal of the dye column operations have been carried out and breakthrough capacities of the Bottom Ash and De-Oiled Soya columns have been calculated. Attempts have also been made for the recovery of the adsorbed dye from exhausted columns by eluting dilute NaOH and more than 90% of the dye was recovered

  2. Bazı Oksidan Maddeler ve Emülgatör ile Birlikte Katılan Soya Ununun Hamurun Reolojik Özellikleri Üzerine Etkisi

    Directory of Open Access Journals (Sweden)

    Recai Ercan

    2015-02-01

    Full Text Available Diyetleri hububat ya da nişastalı gıdalardan oluşan kişilerin önemli bir sorunu olan protein yetersizliği, ekmek veya diğer nişastalı ürünlerin proteince zengin katkı maddeleriyle kuvvetlendirilmesini gerektirmektedir. Bu amaçla fiyatı, kalitesi ve miktarı ve kullanımındaki kolaylığına bağlı olarak en çok soya unu kullanılmaktadır. Bu çalışmanın amacı tek tek, ya da kombinasyonlar halinde tam yağlı soya unu, SSL, bromat ve L. askorbik asit kullanılarak zayıf un hamurlarının fiziksel özelliklerini geliştirmektir. Sonuçlar ilave edilecek soya unu ve SSL miktarının un kalitesi ile ilişkili olduğunu ve soya unu ile SSL yerine bromat ya da L. askorbik asit kullanılması ihtimalinin oldukça düşük olduğunu göstermiştir.

  3. EPOXIDACIÓN DE OLEÍNA DE PALMA CON ÁCIDO PEROXIACÉTICO FORMADO IN SITU

    Directory of Open Access Journals (Sweden)

    ERNESTO ZULETA S

    Full Text Available Este artículo presenta los resultados de un proyecto de investigación encaminado a evaluar las condiciones de producción de oleína de palma epoxidada mediante el método de ácido peroxiacético formado in situ. Los aceites epoxidados son sustancias químicas de gran importancia industrial, de uso directo como plastificantes, o como intermediarios en la fabricación de otros productos químicos de mayor valor agregado. Este trabajo realiza un aporte investigativo a nivel regional, brindando una alternativa de uso para la oleína de palma africana mediante su utilización en la obtención de un producto con alta potencialidad de producción y utilidad como son los aceites epoxidados. Bajo las condiciones de estudio, se pudo obtener una oleína de palma epoxidada con un porcentaje de oxígeno oxiranode 3,007%, valor comparable con el valor requerido para un aceite de soya epoxidado comercial según la normatividad nacional

  4. Cementos con cenizas volantes

    Directory of Open Access Journals (Sweden)

    Ossa M., Mauricio

    1984-03-01

    additions of 20 and 30% .

    Casi la generalidad de los estudios realizados sobre cementos con adición de cenizas volantes se refieren a sus características y comportamiento en pastas, morteros y hormigones, siempre en relación con aquéllos del cemento portland. Esta vez, se desarrolló un trabajo experimental orientado a relacionar entre sí los cementos con adiciones de cenizas volantes y de puzolana natural. Para ello se fabricaron a escala de laboratorio cementos de ambos tipos, empleando como materias primas comunes clinker y yeso y, como variables, diferentes porcentajes de las dos adiciones, que cumplieron previamente los requisitos normalizados en cuanto a sus actividades puzolánicas. La calidad de los cementos fabricados resultó adecuada y concordante con la del cemento portland-puzolánico obtenido a escala industrial con los mismos clinker, yeso y puzolana natural de este estudio. Posteriormente, se determinaron las características de los cementos experimentales y se confeccionaron morteros normales para la realización de ensayos físicos y mecánicos. Los resultados de ensayos indicaron que los cementos con adición de cenizas volantes (CCV requieren menos agua para consistencia normal, presentan tiempos de fraguado mayores y expansiones en autoclave menores que los cementos con adición de puzolana (CP. Los calores de hidratación a 7 y 28 días de edad fueron aproximadamente similares para ambos tipos de cemento. En morteros normales, los cementos CCV mostraron menor retracción de secado, mayor retentividad y mayor fluidez (para igual cantidad de agua que los cementos CP. En los ensayos de exudación se observó que ésta depende más de la finura que el tipo de adición. Finalmente, los ensayos mecánicos señalaron que las resistencias a compresión y flexotracción de los morteros con cementos CCV son menores a edades inferiores que 14 días (del orden de 5 a 10% a un día de edad, pero que a partir de entonces pasan a ser mayores que las de

  5. competencia con China

    Directory of Open Access Journals (Sweden)

    Elena de la Paz Hernández Águila

    2007-01-01

    Full Text Available El artículo ofrece un diagnóstico del desempeño de la industria mexicana del calzado desde la década de los ochenta hasta la actualidad. Analiza la problemática que ha enfrentado esta rama industrial a partir del proceso de apertura comercial y de la competencia en su mercado interno con productos provenientes de países asiáticos, particularmente China. Problematiza al respecto los retos y las perspectivas que a mediano plazo enfrentará este sector empresarial y sobre las posibilidades de competir en el mercado globalizado.

  6. Construir con Madera

    OpenAIRE

    Olabe-Velasco, F. (Fermín); Val-Hernández, Y. (Yolanda); Varela-de-la-Cruz, P. (Perla); Cabrero-Ballarín, J.M. (José Manuel)

    2010-01-01

    Guía divulgativa ‘Construir con madera’, elaborada por la Cátedra Madera de la Universidad de Navarra y el Gobierno de Navarra. La publicación pretende explicar de forma sencilla los beneficios y posibilidades de este material en la construcción, tanto en lo que respecta a su resistencia, comportamiento frente al fuego, durabilidad, capacidad de aislamiento, propiedades acústicas, estética, respeto al medio ambiente y sostenibilidad como fuente de energía. A modo de ejemplo, en la ...

  7. Newly formulated, protein quality-enhanced, extruded sorghum-, cowpea-, corn-, soya-, sugar- and oil-containing fortified-blended foods lead to adequate vitamin A and iron outcomes and improved growth compared with non-extruded CSB+ in rats.

    Science.gov (United States)

    Delimont, Nicole M; Fiorentino, Nicole M; Opoku-Acheampong, Alexander B; Joseph, Michael V; Guo, Qingbin; Alavi, Sajid; Lindshield, Brian L

    2017-01-01

    Corn and soyabean micronutrient-fortified-blended foods (FBF) are commonly used for food aid. Sorghum and cowpeas have been suggested as alternative commodities because they are drought tolerant, can be grown in many localities, and are not genetically modified. Change in formulation of blends may improve protein quality, vitamin A and Fe availability of FBF. The primary objective of this study was to compare protein efficiency, Fe and vitamin A availability of newly formulated extruded sorghum-, cowpea-, soya- and corn-based FBF, along with a current, non-extruded United States Agency for International Development (USAID) corn and soya blend FBF (CSB+). A second objective was to compare protein efficiency of whey protein concentrate (WPC) and soya protein isolate (SPI) containing FBF to determine whether WPC inclusion improved outcomes. Eight groups of growing rats ( n 10) consumed two white and one red sorghum-cowpea (WSC1 + WPC, WSC2 + WPC, RSC + WPC), white sorghum-soya (WSS + WPC) and corn-soya (CSB14 + WPC) extruded WPC-containing FBF, an extruded white sorghum-cowpea with SPI (WSC1 + SPI), non-extruded CSB+, and American Institute of Nutrition (AIN)-93G, a weanling rat diet, for 4 weeks. There were no significant differences in protein efficiency, Fe or vitamin A outcomes between WPC FBF groups. The CSB+ group consumed significantly less food, gained significantly less weight, and had significantly lower energy efficiency, protein efficiency and length, compared with all other groups. Compared with WSC1 + WPC, the WSC1 + SPI FBF group had significantly lower energy efficiency, protein efficiency and weight gain. These results suggest that a variety of commodities can be used in the formulation of FBF, and that newly formulated extruded FBF are of better nutritional quality than non-extruded CSB+.

  8. Efecto de la concentración de goma de semillas de mezquite y proteínas de soya en la microestructura de coágulos obtenidos de leche

    Directory of Open Access Journals (Sweden)

    Araceli Hernández T.

    2003-01-01

    Full Text Available Desde hace algunas décadas y por muchas razones, existe el interés por la incorporación de biopolímeros en la matriz de caseína, tales como proteínas y polisacáridos. Dicho proceso ha logrado aumentar rendimientos, reducir calorías o desarrollar atributos especiales. En la mayoría de los casos, esta adición se hizo mezclando los biopolímeros en coágulos de leche previamente obtenidos. El artículo trata acerca de la incorporación de la goma de semillas de mezquite (GSMez y las proteínas de soya (PS en la leche antes de la coagulación y su efecto sobre la matriz protéica de caseína. Lo anterior se realizó por método enzimático, con cuajo líquido microbiano. Se emplearon concentraciones de 0.15 y 0.45 % (p/v para GSMez y de 0.6 y 1.5 % (p/v para PS. Se observó que conforme se incrementaba la concentración de GSMez y de PS se dificultaba más la coagulación y había formación de cúmulos de caseína más pequeños. Tanto la GSMez como la PS presentaron concentración crítica arriba de la cual no se produce la coagulación, lo que genera una suspensión arenosa. Los coágulos prensados de GSMez, obtenidos justo en concentración crítica, mostraron una apariencia suave y húmeda, mientras que los adicionados de PS, destacan por su apariencia seca, dura y arenosa. Las observaciones concluyeron que tanto la GSMez como la PS producen un impedimento de tipo estérico que no permite la correcta adhesión de las caseínas para formar el coágulo. Este efecto limita el uso de estos biopolímeros a concentraciones mayores de 0.15% (p/v para GSMez y 0.6 % (p/v para PS

  9. Hiperinmunoglobulinemia E: una inmunodeficiencia primaria con compromiso de varios mecanismos de la respuesta inmune

    Directory of Open Access Journals (Sweden)

    Juan E. Pérez

    1998-02-01

    Full Text Available El síndrome de hiperinmunoglobulinemia E(SHIE es una Inmunodeficiencia Primaria (IOP de origen desconocido, cuya fisiopatología no ha sido -esclarecida. Sus manifestaciones comienzan en las primeras semanas de vida. Es característico en su expresión clínica el desarrollo de un síndrome de infección recurrente patológica, con neumopatía supurativa y neumatoceles perennes principalmente producidos porStaphylococcusaureus; se encuentran niveles séricos muy elevados de IgE. En nuestras investigaciones sobre la patogénesis del ?HIE, la hipótesis propone varias alteraciones como causas importantes para su presentación: Trastornos en la activación y generación de memoria de los linfocitos T, posiblemente debida a una expresión e interacción inadecuadas de las moléculas de superficie involucradas en este fenómeno (CO40L, CO80, CO86, CTLA-4; un patrón de citoquinas diferente, con producción incrementada de IL-13 que sería la responsable del exceso de síntesis de la IgE y del factor estimulante de colonias de granulocitos y monolitos G M-CSF (G M-CS F= Granulocyte-Monocyte Colony Stimulating Factor el cual modularía la alteración en la función de las células fagocíticas.

  10. Entrevista con Giovanni Levi

    Directory of Open Access Journals (Sweden)

    Monica Oliveira

    2017-06-01

    Full Text Available En esta entrevista, Giovanni Levi - como un conocedor del tema de Familia - realiza una importante evaluación sobre el actual estado de las investigaciones realizadas en el Brasil y em el exterior. Con estilo franco, agudo y lucido critica las visiones tradicionales y sus ilusiones ypropone nuevos conceptos y métodos. La historia de la familia debería ceder espacio para el estudio de las redes relacionales o de los mundos relacionales. De la misma forma, la historia cuantitativa debería abrir espacio para el estudio de las cualidades. Ya con relación a la historia de las elites, tan estudiada y reproducida en una diversidad de trabajos, que deberíase mirar en otra perspectiva. Es decir, no mirar a las reglas sociales predeterminadas, sino a los desvíos y a las variaciones. Levi defiende que los historiadores deben trascender a los documentos que se encuentran fácilmente y que pueden fortalecer perspectivas deformadas y esequilibradas de la sociedad. Para él, los historiadores deben esforzarse por estudiar a aquellos grupos que dejaron pocos rastros documentales. En ese esfuerzo existiría una nueva mirada sobre la historia de la familia.

  11. Entrevista con Patricia Ariza

    Directory of Open Access Journals (Sweden)

    Esperanza Londoño La Rotta

    2017-01-01

    Full Text Available Pensamiento, Palabra y Obra entrevista a una artista, feminista y activista política, quien como mujer y artista ha permitido pensar el arte más allá de un simple espectáculo. Toda una vida dedicada al teatro y a darle voz, a través de sus obras, a víctimas del conflicto colombiano, defensora de derechos humanos; además de hacer evidente en su vida y a través de la plataforma “Artistas por la paz”, las múltiples relaciones que se pueden establecer entre el arte, la construcción de paz y la resolución de conflictos. Hablamos en su casa, en medio del calor de la bienvenida con Patricia Ariza, directora del festival alternativo de teatro, de Mujeres en Escena y de la Corporación Colombiana de Teatro, entre otras muchas actividades que voluntariamente su espíritu libertario ha asumido. Esta entrevista se realizó antes del 2 de octubre, pero con la revisión de los acuerdos que propició el plebiscito ganado por una ínfima minoría por el no, sigue siendo vigente este planteamiento.

  12. Efecto de la humedad y compactación de un Ultisol de la sabana del estado Monagas sobre la concentración de clorofila y carotenoides, lavado de electrolitos y contenido relativo de agua en plantas de soya Efecto de la humedad y compactación de un Ultisol de la sabana del estado Monagas sobre la concentración de clorofila y carotenoides, lavado de electrolitos y contenido relativo de agua en plantas de soya

    Directory of Open Access Journals (Sweden)

    Francisco J. Parra D.

    2012-02-01

    Full Text Available In late 2008, a greenhouse trial was conducted with soybeans to determine the behavior ofchlorophyll a, b, a+b and carotenoid concentration , electrolyte leakage, and relative watercontent with respect to moisture and ultisol soil compaction, because these two factors are acommon limiting factor of agricultural soil in the state of Monagas. A randomized block designwith factorial arrangement (4x4 was used, and the interaction of four levels of compactionproduced by different number of blows per layer (0, 12, 24, 36 and four levels of moistureproduced by varying the irrigation frequency (daily, every two days, every three days and fourdays was studied. Analysis of variance and regression analysis indicated that the most influentialfactor in the variables assessed was the frequency of irrigation.A finales del año 2008, se realizó un ensayo a nivel de invernadero para determinar el comportamiento de la concentración de clorofila a, b, a+b y carotenoides, el lavado de electrolitos y contenido relativo de agua en el cultivo de Soya, con respecto a la humedad y compactación de un suelo Ultisol, debido a que estos dos factores son una limitante muy común en los suelos agrícolas del estado Monagas. Se trabajó bajo un diseño de bloques al azar en arreglo factorial (4 x 4 donde se estudió la interacción de cuatro niveles de compactación a través de diferentes número de golpes por capa (0, 12, 24, 36 y cuatro niveles de humedad a través de la variación de las frecuencias de riego (todos los días, cada dos días, cada tres días y cada cuatro días, luego del análisis de varianza y posterior análisis de regresión se concluyó que el factor más influyente en las variables evaluadas fue la frecuencia de riego.

  13. Interaction of sodium diclofenac with freeze-dried soya phosphatidylcholine and unilamellar liposomes Interação do diclofenaco com fosfatidilcolina de soja e lipossomas unilamelares liofilizados

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    Luciana Biagini Lopes

    2006-12-01

    Full Text Available Phospholipids are widely used as structural amphiphilic compounds in liposome formulations. In this study, we have analyzed the interaction the sodium diclofenac (SD with soya phosphaditylcholine (PC and soya phosphatidylcholine from lyophilized small unilamellar liposomes (SUV. The changes in the properties of the co-lyophilized drug/PC from SUV liposomes, lyophilized PC from SUV liposomes, and lyophilized soya phosphatidylcholine, were studied by Differential Scanning Calorimetry (DSC. The DSC data showed that the previous organization of phospholipids molecules to form liposome affects intensely the thermal behavior of PC when compared to non-lipossomal PC. SD modified the thermal properties of PC from liposomes. It was verified that SD affects intensely the located group peaks in the regions of 120-140 ºC and in the higher temperature region of 240-260 ºC. The results of this work demonstrated that the presence of the drug modified the DSC behavior for both liposomal and non-liposomal PC and that these modifications can be easily monitored by DSC analysis.Fosfolipídios são freqüentemente usados como compostos anfifílicos estruturais em formulações de lipossomas. Neste estudo foi analisada a interação do diclofenaco sódico (SD com a fosfatidilcolina de soja liofilizada e a fosfatidilcolina de soja (PC obtida de lipossomas unilamelares pequenos liofilizados (SUV. As modificações nas propriedades da mistura fármaco/PC co-liofilizados a partir de SUV pré-formados, lipossomas de PC vazios e PC liofilizada foram estudadas por Calorimetria Diferencial de Varredura (DSC. Os resultados de DSC mostraram que a organização prévia das moléculas PC para formar lipossomas interfere significativamente no perfil de DSC da PC, quando comparada ao perfil de DSC da PC não-lipossômica. Verificou-se que o SD afeta intensamente o grupo de picos situados nas regiões de 120-140ºC e na região de mais alta temperatura (240-260ºC. Os resultados

  14. Cinética de fermentación y degradabilidad ruminal in vitro de dietas con diferente fuente de nitrógeno

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    Carlos Aguirre Valverde

    2017-12-01

    Full Text Available El objetivo de este trabajo fue determinar la cinética de fermentación y degradabilidad ruminal in vitro de dietas con diferente fuente de nitrógeno para alimentación de rumiantes. Se formularon tres dietas (D con diferentes fuentes de nitrógeno D1 (harina de pescado, D2 (pasta de soya y D3 (urea, estas fueron iso-calóricas con 2900 kilocalorías de energía digestible en materia seca (kcal ED/MS e iso-proteícas 13% proteína bruta (PB. Los periodos de incubación utilizados fueron 0, 2, 4, 8, 12, 24, 48 y 72 horas. En la evolución del Ph en la cinética ruminal de las tres dietas con diferentes fuentes de nitrógenos, se puede determinar que la D3 y D1 registraron el mayor pH (P

  15. Influence of diets to Wistar rats supplemented with soya, flaxseed and lupine products treated by lactofermentation to improve their gut health.

    Science.gov (United States)

    Bartkiene, E; Juodeikiene, G; Vidmantiene, D; Zdunczyk, Z; Zdunczyk, P; Juskiewicz, J; Cizeikiene, D; Matusevicius, P

    2013-09-01

    The present study proposes the contribution of lactic acid bacteria and plants rich in bioactive substances and high-quality proteins as alternative products for human diets in improving the gut environment as potential against pathogenic bacteria. The effect of diets supplemented with soya, flaxseed and lupine flours fermented with a Pediococcus acidilactici KTU05-7 probiotic strain in the gastrointestinal tract (GIT) of Wistar rats were analyzed. In vivo experiments showed a positive effect of long time lactofermentation of plant material on the body weight of rats. Diets with fermented yellow lupine resulted in enhanced activities of α-glucosidase, β-galactosidases, as well as high levels of lactic acid bacteria, bifidobacteria and enterococci in the GIT were determined. Lactofermentation of analyzed plant products had a significantly lowering effect on Escherichia coli compared with the control group. The dominant flora of large intestines like Bifidobacterium and anaerobic cocci were found in high levels after diets with fermented lupine.

  16. preescolares desnutridos con madres con obesidad y sin obesidad

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    Viridiana Vanessa Conzuelo-González

    2009-01-01

    Full Text Available El primer objetivo fue conocer cuántos menores de cinco años con diferentes grados de desnutrición tienen una madre con sobrepeso/obesidad/ en una comunidad indígena que vive en extrema pobreza y bajo condiciones de migración masculina internacional. El segundo fue comparar tres variables socionutricionales (ingreso familiar, educación de la madre y adecuación nutrimental de la dieta diaria entre estos hogares y los hogares con desnutrición infantil y madres sin obesidad. Se realizó un estudio transversal (2006-2007, en la comunidad mazahua de San Francisco Tepeolulco, Municipio de Temascalcingo; que incluyó a 85 hogares integrados por preescolares con desnutrición inscritos al programa Oportunidades. Se determinó el estado nutrición de los preescolares con indicadores antropométricos y se obtuvo el IMC de las madres de estos infantes. Se aplicó una encuesta socionutricional, incluida el recordatorio de 24 horas, y complementado con la observación participante (cualitativa. Se encontró que 83% de las madres mazahuas presentaron sobrepeso u obesidad. El estado de nutrición de los preescolares con madres con obesidad presentó un porcentaje mayor de desnutrición (76%. En la variable género, se encontró que 54% de los niños con madres con obesidad tenía baja talla. Al relacionar el nivel educativo de la madre, esta variable resultó ser estadísticamente significativa (p=0.015, donde el analfabetismo está más relacionado con la desnutrición infantil que tienen madres de bajo y/o peso normal. La elevada prevalencia de hogares conformados con preescolares con desnutrición y madres con obesidad, es un síntoma más de la pobreza en zonas indígenas en México, con bajo índice de desarrollo humano.

  17. Pollos cuello desnudo alimentados con harina de Morus alba y Cajanus cajan

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    Tito Solís-Barros

    2017-12-01

    Full Text Available El objetivo de la investigación fue estudiar el comportamiento productivo de pollos cuello desnudo heterocigotos que se alimentaron con harina de hojas de Morus alba y granos de Cajanus cajan tostados a 120 ºC por 12 min, se utilizaron 144 pollos sexados de la estirpe T451N de 1 a 90 d de edad. Se distribuyeron según diseño de bloques al azar, en tres tratamientos, 48 pollos por tratamiento repartidos en ocho réplicas. Fueron criados en piso, en instalaciones rústicas. Se evaluaron indicadores productivos como el consumo de alimento, ganancia de peso promedio diario, conversión alimentaria y la eficiencia en el uso de los nutrientes. Los tratamientos fueron: T1=Control, T2=5% Cajanus cajan y 3% Morus alba y T3=3% C. cajan y 5% M. alba. El control consumió maíz y soya, como dieta base. La mayor ingestión de alimento (p

  18. autorregulado con estudiantes universitarios

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    Jairo Andrés Montes

    2005-01-01

    Full Text Available El propósito del presente estudio es describir la forma en la que se presentan los procesos de aprendizaje autorregulado con un grupo de estudiantes (22 estudiantes de tercer semestre de Psicología de la PUJ, Cali, en el evento de preparación para la presentación un examen. Asimismo se describen las correlaciones que ocurren entre las distintas fases de dicho proceso de autorregulación del aprendizaje. Para conseguir los objetivos propuestos se ha hecho uso de una observación de desempeño en tiempo real, es decir, de la observación durante una sesión de preparación de examen de los estudiantes, en la cual se emplearon protocolos verbales para dar cuenta de lo que «pasaba por su mente» mientras estudiaban. Una entrevista semi-estructurada y una prueba objetiva. Los resultados fueron analizados a la luz del modelo mixto de procesamiento de información y constructivismo abordado por Winne(1998. Como resultado se encontró una relación significativa entre los niveles de desempeño en el proceso de ARR y el resultado del examen. Igualmente se encontraron bajos niveles de regulación en una parte importante de la muestra y un desfase significativo entre conocimiento declarativo de ARR y desempeño en el mismo

  19. Puentes con vigas pretensadas

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    Editorial, Equipo

    1965-07-01

    Full Text Available This paper describes one of the three bridges which Hidrocivil, S. A., has built in Catalonia (northern Spain, over the river Ripoll. The other two bridges are very similar to this one, both in construction and design, and show only minor adjustments to the local topography. The contracting firm proposed several alterations in the prefabrication and constructional procedure, in relation to the initial project, and these changes were accepted. The main feature of these projects is the use of prestressed beams, built at the workshop in sections, and joined together by means of sixty 7 mm cables in each beam. As the shear forces are more acute at the joints, the end of each section has a kind of diaphragm, to provide a large contact area, and hence greater surface to transmit the shear forces. The methods of construction are also of interest. Briefly, they involve building the bridge piles, and use these to support a provisional structure with transversal movement. This provisional structure, in turn, served as platform for two bridge cranes, which lifted the girders to their final location. After the first span was completed, the deck was concreted and the auxiliary structure pushed forward to the next span, to repeat the same operations. This arrangement saved the use of provisional framework.En este trabajo se describe uno de los tres puentes que Hidrocivil, S. A., ha construido.—previo concurso— en la región catalana; concretamente, el que salva el río Ripoll. Los otros dos no han sido objeto de descripción general por ser muy similares, en lo que a ejecución y concepción se refiere, con la única variante que presentan las características topográficas locales. La empresa propuso ciertas variantes— que fueron aceptadas— en la prefabricación y métodos de construcción. El interés de estas obras se centra en el empleo de vigas pretensadas, prefabricadas en taller por trozos, y solidarizados en el mismo mediante las operaciones

  20. Violencia con el anciano

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    Rita Campillo Motilva

    2002-08-01

    Full Text Available La violencia doméstica es tan antigua como la humanidad misma y se reconocen la violencia infantil, contra la mujer y al anciano, fundamentalmente; siendo este último grupo una población en ascenso por las mayores expectativas de vida de los últimos años. Como resultado de ello, el número de casos de abuso en el anciano se incrementará y el impacto de este abuso sobre la salud debe ser considerado de forma adecuada. La gama de maltratos es variadísima e incluye el abuso físico, emocional, financiero, sexual, por negligencia, negación a brindarle ayuda y otras formas más. Los ancianos con deterioro cognitivo son los más vulnerables. El médico en la atención primaria de salud es un pilar importante en la prevención y educación de este problema.Domestic violence is as old as humanity itself. Child, women and elderly abuse are mainly recognized. The elderly group is increasing due to the higher life expectancy experimented during the last years. As a result, the number of battered elderly will grow and the impact of this abuse on health should be adequately considered. The range of abuse is very wide and it includes physical, emotional, financial and sexual abuse, negligence, rejection to give assistance and others. The elderly with cognitive deterioration are the most vulnerable. The physician at the primary health care level is an important milestone in the prevention and education of this problem.

  1. Influence of pre-sowing irradiation of soya seeds with low doses of gamma rays on the yields of grain and on the content of crude protein in the grain

    International Nuclear Information System (INIS)

    Nikolov, Ch.V.

    1985-01-01

    Pre-sowing irradiation of air-dry soya seeds of the Hodson variety, calibrated in size and humidity (12%), with gamma rays in the range of relatively low intensities of irradiation of 0.27 to 5 Gy/min and doses of 10 to 20 Gy increases both the yield of grain and the content of crude protein in the grain in relation to the absolute dry matter. The dependence of radiostimulation effect on the factors of the environment cannot be reason for neglecting it as a posssible reserve for increasing the yield of grain from soya and the content of crude protein in the grain. Possible results are exspected from production experiments with pre-sowing irradiation of seeds of Hodson variety using gamma rays in the range of the above intensities and doses

  2. Intakes and sources of isoflavones, lignans, enterolignans, coumestrol and soya-containing foods in the Norfolk arm of the European Prospective Investigation into Cancer and Nutrition (EPIC-Norfolk), from 7 d food diaries, using a newly updated database.

    Science.gov (United States)

    Mulligan, Angela A; Kuhnle, Gunter G C; Lentjes, Marleen A H; van Scheltinga, Veronica; Powell, Natasha A; McTaggart, Alison; Bhaniani, Amit; Khaw, Kay-Tee

    2013-08-01

    A diet rich in phyto-oestrogens has been suggested to protect against a variety of common diseases but UK intake data on phyto-oestrogens or their food sources are sparse. The present study estimates the average intakes of isoflavones, lignans, enterolignans and coumestrol from 7 d food diaries and provides data on total isoflavone, lignan and phyto-oestrogen consumption by food group. Development of a food composition database for twelve phyto-oestrogens and analysis of soya food and phyto-oestrogen consumption in a populationbased study. Men and women, aged 40–79 years, from the general population participating in the Norfolk arm of the European Prospective Investigation into Cancer and Nutrition (EPIC-Norfolk) between 1993 and 1997, with nutrient and food data from 7 d food diaries. A subset of 20 437 participants. The median daily phyto-oestrogen intake for all men was 1199 mg (interquartile range 934–1537mg; mean 1504mg, SD 1502mg) and 888mg for all women (interquartile range 710–1135 mg; mean 1205 mg, SD 1701mg). In soya consumers, median daily intakes were higher: 2861 mg in men (interquartile range 1304–7269mg; mean 5051mg, SD 5031mg) and 3142 mg in women (interquartile range 1089–7327mg; mean 5396 mg, SD 6092 mg). In both men and women, bread made the greatest contribution to phyto-oestrogen intake – 40?8% and 35?6%, respectively. In soya consumers, vegetable dishes and soya/goat’s/sheep’s milks were the main contributors – 45?7% and 21?3% in men and 38?4% and 33?7% in women, respectively. The ability to estimate phyto-oestrogen intake in Western populations more accurately will aid investigations into their suggested effects on health.

  3. La historia del uso de la soya en México, su valor nutricional y su efecto en la salud The importance of soy in Mexico, its nutritional value and effect on health

    Directory of Open Access Journals (Sweden)

    Nimbe Torres y Torres

    2009-06-01

    Full Text Available La proteína de soya se ha utilizado en algunos países latinoamericanos, incluido México, en diversos programas de alimentación; el propósito ha sido el de mejorar el estado nutricional de la población debido a su elevado valor nutricio y su costo relativamente bajo que mantuvo por algún tiempo. En este trabajo se describen los usos y la investigación que se ha llevado a cabo sobre la soya en México desde 1970. Además, se revisan los efectos de los diferentes componentes de la soya, en particular de su proteína y las isoflavonas, sobre la salud, su mecanismo de acción en la reducción del colesterol y los triglicéridos y la disminución de la secreción de insulina, así como su respuesta glucémica; por último, se delinean las recomendaciones del consumo de la proteína de soya parar obtener un beneficio en la salud.Soy protein has been used in several Latin-American countries including Mexico in various food programs with the purpose to improve the nutritional status of the population due to the high nutritional value and its relatively low cost that was maintained for some time. In the present work, the use and research on soy in Mexico since 1970 is described. In addition, this review shows the effects of the different components of soy, in particular its protein and the isoflavones on health, the mechanism of action of soy protein to reduce cholesterol and triglycerides, and insulin secretion, as well as its glycemic response, and finally, the recommendations of soy protein consumption to obtain a health benefit.

  4. Soya ve Buğday Rüşeymi Katkılı Unların Kalitesini Düzeltme İmkanları Üzerinde Araştırmalar

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    Berrin Kahveci

    2015-02-01

    Full Text Available Araştırmada üç ayrı kalitedeki una, ısıl işlem uygulanmış muhtelif oranlarda tam yağlı ve yağsız soya veya tam yağlı ve yağsız buğday rüşeymi katılarak bunların ekmeklik kalitesine etkileri araştırılmıştır. Sonra soya veya buğday rüşeymi katkılı örneklere %0.2 ve %0.4 oranında SSL katılarak bunun ekmek özelliklerini düzeltici etkileri tespit edilmiştir. Soya ve rüşeym katkıları ekmeklerde hacim verimini ve Dallmann değerini düşürmüş, gözenek yapısını olumsuz yönde etkilemiştir. Genelde soyanın ekmek kalitesine olumsuz etkileri, buğday rüşeymine kıyasla daha az olmuştur. SSL katkısı örneklerin ekmeklik özelliklerini önemli ölçüde iyileştirmiştir.

  5. Mapping of T cell epitopes of the major fraction of rye grass using peripheral blood mononuclear cells from atopics and non-atopics. II. Isoallergen clone 5A of Lolium perenne group I (Lol p I).

    Science.gov (United States)

    Bungy, G A; Rodda, S; Roitt, I; Brostoff, J

    1994-09-01

    Rye grass is the major cause of hay fever which currently affects 20% of the population. Lolium perenne group I (Lol p I) is a glycoprotein of 240 amino acid residues, representing the main allergen of rye grass. We have used peripheral blood mononuclear cells (PBMC) from controls and subjects allergic to rye grass and cultured them with L. perenne extract (LPE) and Lol p I and measured lymphocyte activation using thymidine incorporation. Patients were further studied against the 115 overlapping peptides of the iso-allergen clone 5A of Lol p I to see whether the 4 amino acid residue differences between clone 1A and clone 5A affect the T cell epitope and thus, lymphocyte activation. There are 24 peptide differences between isoallergen clone 1A and clone 5A occurring in pools 4, 13, 16 and 19 each one of which could be an immunodominant epitope. The PBMC from all allergic patients studied showed a strong proliferative response to LPE and Lol p I. Five immunogenic peptide pools, pool 6, 15, 16, 17 and 19 of the isoallergen clone 5A were also identified. Most of these pools are in the C-terminal region of Lol p I. Out of 20 pools tested in vitro 1 pool (pool-17) induced PBMC proliferation in five out of six patients who were not restricted to an HLA class II DR gene product. However, three out of the six subjects responded to various other peptide pools in addition to the immunodominant pool. In spite of the amino acid differences between the two clones, pool 17 still remains the immunodominant T cell epitope. Control subjects showed only weak responses to LPE and no detectable response to either Lol p I or peptide pools. From within the most active pool we have defined two peptides of the isoallergen clone 5A (identical in sequence with clone 1A) which stimulate lymphocytes from rye grass-sensitive patients in vitro. Previous studies with the two continuous sequences (193WGAVWRIDTPDK204 and 195AVWRIDTPDKLT206) tested in vivo by intradermal skin testing have shown

  6. Efecto de la temperatura de almacenamiento sobre la viabilidad de bacterias simbióticas fijadoras de nitrógeno utilizadas en la elaboración de inoculantes biológicos para arveja (Pisum sativum y soya (Glycine max

    Directory of Open Access Journals (Sweden)

    Lina M. Moreno Conn

    2014-07-01

    Full Text Available El nitrógeno es un elemento requerido en grandes cantidades por los cultivos, sin embargo, en el suelo es uno de los elementos más limitantes ya que se encuentra en formas poco disponibles para las plantas y debe ser incorporado a través de la fertilización química o por medio de la fijación biológica llevada a cabo por microorganismos. Las bacterias simbióticas fijadoras de nitrógeno han sido ampliamente utilizadas para la elaboración de inoculantes, constituyéndose en una alternativa viable para mejorar el rendimiento de los cultivos a través de un mejor suministro de este elemento. Estos inoculantes para que puedan ser comercializados deben cumplir con ciertos requisitos de calidad que garanticen el buen funcionamiento del producto. En este estudio se evalúo la viabilidad de las cepas ICA L9 e ICA J96 en inoculantes almacenados a temperaturas de 4±2, 18±3 y 28±2 °C durante 180 días y la actividad biológica en plantas de arveja y soya. Las cepas utilizadas pertenecen al Banco de Germoplasma de Microorganismos de Corpoica; para los ensayos biológicos se emplearon semillas de arveja variedad “Santa Isabel” y soya variedad “Corpoica Superior 6”. Al evaluar la viabilidad de los inoculantes almacenados a 4±2 °C, 18±3 °C y 28±2 °C, se evidenció que la temperatura no afectó la supervivencia y la concentración de rizobios después de 180 días de almacenamiento permitiendo observar un número de unidades formadoras de colonias por gramo superior a 108, valor que garantiza la calidad del inoculante. En cuanto a la actividad biológica, se observó que las cepas inoculadas fueron infectivas y efectivas para la fijación biológica del nitrógeno, comparado con los testigos absolutos. Nitrogen is an element required in large amounts by most crops, however, in soil is one of the most limiting and located in ways not available to the plant and must be incorporated through chemical fertilization or by biological

  7. Acne polimorfo: tratamiento con Implacen

    Directory of Open Access Journals (Sweden)

    Rubén Pérez Armas

    1995-06-01

    Full Text Available Se realiza el estudio de 40 pacientes con acné polimorfo, los que fueron atendidos en la Consulta de Dermatología del Hospital Provincial Clinicoquirúrgico Docente "Celia Sánchez Manduley", en el período comprendido de enero de 1988 a diciembre de 1989. Se revisa la literatura médica sobre los diversos métodos y medicamentos utilizados en la terapéutica de esta dermatosis. Se describe el esquema de tratamiento empleado con implacén en 30 pacientes; los 10 restantes se trataron con placebo; se compara dicho esquema con los tradicionales y se observan mejores resultados con nuestro estudio. Se destaca la ausencia de recaídas, así como el resultado del tratamiento de acuerdo con el sexo.A study was performed in 40 patients presenting with polymorphic acne who were attended in the Dermatology Department of "Celia Sánchez Manduley" Clinicosurgical and Teaching Hospital from January, 1988 to December, 1989. A review of the literature was made seeking for the different methods and drugs used for the treatment of this dermatosis. The treatment schedule with the use of implacen in 30 patients is described. Such therapeutic schedule was compared with traditional ones and better results were observed with the use of implacen. The fact that there were no relapses is highlighted, as well as the result of treatment according to sex.

  8. Dairy cow responses to graded levels of rapeseed and soya bean expeller supplementation on a red clover/grass silage-based diet.

    Science.gov (United States)

    Rinne, M; Kuoppala, K; Ahvenjärvi, S; Vanhatalo, A

    2015-12-01

    The effects of rapeseed and soya bean expeller (SBE) supplementation on digestion and milk production responses in dairy cows were investigated in an incomplete Latin square design using five cows and four 3-week periods. The experimental diets consisted of five concentrate treatments fed at a rate of 9 kg/day: a mixture of barley and oats, which was replaced with rapeseed or SBE at two levels (CP concentration (g/kg dry matter (DM)) of 130 for the control concentrate and 180 and 230 for the two protein supplemented levels). A mixture of grass and red clover silage (1:1) was fed ad libitum and it had a CP concentration of 157 g/kg DM. Supply of nutrients to the lower tract was measured using the omasal canal sampling technique, and total digestion from total faecal collection. Protein supplementation increased omasal canal amino acid (AA) flows and plasma concentrations of AA, and was also reflected as increased milk production. However, N use efficiency (NUE) decreased with increased protein supplementation. Rapeseed expeller (RSE) tended to increase silage DM intake and elicited higher milk production responses compared with SBE and also resulted in a higher NUE. The differences between the protein supplements in nitrogen metabolism were relatively small, for example, there were no differences in the efficiency of microbial protein synthesis or omasal canal flows of nitrogenous components between them, but plasma methionine concentration was lower for soya bean-fed cows at the high CP level in particular. The lower milk protein production responses to SBE than to RSE supplementation were at least partly caused by increased silage DM and by the lower methionine supply, which may further have been amplified by the use of red clover in the basal diet. Although feed intake, diet digestion, AA supply and milk production were all consistently improved by protein supplementation, there was a simultaneous decrease in NUE. In the current study, the milk protein production

  9. The use of a tannin crude extract from Cistus ladanifer L. to protect soya-bean protein from degradation in the rumen.

    Science.gov (United States)

    Dentinho, M T P; Moreira, O C; Pereira, M S; Bessa, R J B

    2007-06-01

    Cistus ladanifer L. (CL) is a perennial shrub abundant in dry woods and dry land of Mediterranean zone, with high level of tannins. Tannins bind to protein, preventing its degradation in the digestive compartments. This tannin/protein complex may be advantageous when partially protecting good-quality feed protein from excessive rumen protein degradation. The objective of this trial was to use a CL phenol crude extract to prevent excessive rumen degradation of soya-bean meal protein. The phenolic compounds were extracted using an acetone/water solution (70:30, v/v). Soya-bean meal was then treated with this crude CL extract, containing 640 g of total phenols (TP) per kg of dry matter (DM), in order to obtain mixtures with 0, 12.5, 25, 50, 100 and 150 g of TP per kg DM. Three rumen-cannulated rams were used to assess in sacco rumen degradability of DM and nitrogen (N). The three-step in vitro procedure was used to determine intestinal digestibility. Increasing extract concentrations quadratically decreased the N-soluble fraction a (R2 = 0.96, P = 0.0001) and increased the non-soluble degradable fraction b (R2 = 0.92, P = 0.005). The rate of degradation c linearly decreased with CL extract doses (R2 = 0.44, P = 0.0065). For the effective rumen degradability of N, a linear reduction (R2 = 0.94, P < 0.0001) was observed. The in vitro intestinal digestibility of protein (ivID) quadratically decreased (R2 = 0.99, P < 0.0001) with TP inclusion and the rumen undegradable protein (RUP) showed a quadratic increase (R2 = 0.94, P = 0.0417). Total intestinal protein availability, computed from the RUP and ivID, linearly decreased with TP inclusion level (R2 = 0.45, P = 0.0033).

  10. Effects of dehydrated lucerne and soya bean meal on milk production and composition, nutrient digestion, and methane and nitrogen losses in dairy cows receiving two different forages.

    Science.gov (United States)

    Doreau, M; Ferlay, A; Rochette, Y; Martin, C

    2014-03-01

    Dehydrated lucerne is used as a protein source in dairy cow rations, but little is known about the effects of lucerne on greenhouse gas production by animals. Eight Holstein dairy cows (average weight: 582 kg) were used in a replicated 4 × 4 Latin square design. They received diets based on either maize silage (M) or grass silage (G) (45% of diet on dry matter (DM) basis), with either soya bean meal (15% of diet DM) completed with beet pulp (15% of diet DM) (SP) or dehydrated lucerne (L) (30% of diet DM) as protein sources; MSP, ML, GSP and GL diets were calculated to meet energy requirements for milk production by dairy cows and degradable protein for rumen microbes. Dry matter intake (DMI) did not differ among diets (18.0 kg/day DMI); milk production was higher with SP diets than with L diets (26.0 v. 24.1 kg/day), but milk production did not vary with forage type. Milk fatty-acid (FA) composition was modified by both forage and protein sources: L and G diets resulted in less saturated FA, less linoleic acid, more trans-monounsaturated FA, and more linolenic acid than SP and M diets, respectively. Enteric methane (CH4) production, measured by the SF6 tracer method, was higher for G diets than for M diets, but did not differ with protein source. The same effects were observed when CH4 was expressed per kg milk. Minor effects of diets on rumen fermentation pattern were observed. Manure CH4 emissions estimated from faecal organic matter were negatively related to diet digestibility and were thus higher for L than SP diets, and higher for M than G diets; the resulting difference in total CH4 production was small. Owing to diet formulation constraints, N intake was higher for SP than for L diets; interaction between forage type and protein source was significant for N intake. The same statistical effects were found for N in milk. Faecal and urinary N losses were determined from total faeces and urine collection. Faecal N output was lower for M than for G diets but

  11. con mala calidad de vida

    Directory of Open Access Journals (Sweden)

    Agustín Martín-Rodríguez

    2007-01-01

    Full Text Available En este estudio ex post facto se ha analizado si los familiares de pacientes con mala calidad de vida presentan diferencias en las variables clínicas de personalidad y relaciones familiares en función de que el paciente haya estado o no ingresado en una Unidad de Cuidados Intensivos. Seleccionamos dos grupos: 29 familiares de pacientes traumatizados graves transcurridos cuatro años de su ingreso en una UCI de Traumatología y con mala calidad de vida (debido a secuelas físicas y/o psicológicas tras el ingreso, tales como traumatismos craneoencefálicos, politraumatismos y tetraplejias traumáticas y 32 familiares de pacientes con mala calidad de vida con cuatro años de evolución de su enfermedad física (hipertensión, diabetes, artritis reumatoide y síndrome de intestino irritable que no han estado ingresados en la UCI. Para alcanzar nuestro objetivo empleamos una Encuesta Psicosocial y los siguientes instrumentos: Cuestionario de Análisis Clínico, Escala de Clima Social en la Familia y Escala de Adaptación Psicosocial de la Enfermedad. Los resultados mostraron que los familiares de pacientes con mala calidad de vida que estuvieron ingresados en la UCI hace cuatro años, presentan diferencias significativas en las variables agitación y expresividad comparados con los familiares de pacientes con mala calidad de vida que no han estado ingresados en la UCI.

  12. Arbuscular mycorrhizal fungi enhance both absorption and stabilization of Cd by Alfred stonecrop (Sedum alfredii Hance) and perennial ryegrass (Lolium perenne L.) in a Cd-contaminated acidic soil.

    Science.gov (United States)

    Hu, Junli; Wu, Shengchun; Wu, Fuyong; Leung, Ho Man; Lin, Xiangui; Wong, Ming Hung

    2013-10-01

    A greenhouse pot experiment was conducted to compare the phytoextraction efficiencies of Cd by hyper-accumulating Alfred stonecrop (Sedum alfredii Hance) and fast-growing perennial ryegrass (Lolium perenne L.) from a Cd-contaminated (1.6 mg kg(-1)) acidic soil, and their responses to the inoculations of two arbuscular mycorrhizal (AM) fungal strains, Glomus caledonium 90036 (Gc) and Glomus mosseae M47V (Gm). Ryegrass and stonecrop were harvested after growing for 9 and 27 wk, respectively. Without AM fungal inoculation, the weekly Cd extraction by stonecrop (8.0 μg pot(-1)) was 4.3 times higher than that by ryegrass (1.5 μg pot(-1)). Both Gc and Gm significantly increased (P soil acid phosphatase activities, and available P concentrations, and thereby plant P absorptions (except for Gm-inoculated ryegrass), shoot biomasses, and Cd absorptions (except for Gm-inoculated stonecrop), while only Gc-inoculated stonecrop significantly accelerated (P soil pH. The results suggested the potential application of hyper-accumulating Alfred stonecrop associated with AM fungi (notably Gc) for both extraction and stabilization of Cd in the in situ treatment of Cd-contaminated acidic soil. Copyright © 2013 Elsevier Ltd. All rights reserved.

  13. SymB and SymC, two membrane associated proteins, are required for Epichloë festucae hyphal cell-cell fusion and maintenance of a mutualistic interaction with Lolium perenne.

    Science.gov (United States)

    Green, Kimberly A; Becker, Yvonne; Tanaka, Aiko; Takemoto, Daigo; Fitzsimons, Helen L; Seiler, Stephan; Lalucque, Hervé; Silar, Philippe; Scott, Barry

    2017-02-01

    Cell-cell fusion in fungi is required for colony formation, nutrient transfer and signal transduction. Disruption of genes required for hyphal fusion in Epichloë festucae, a mutualistic symbiont of Lolium grasses, severely disrupts the host interaction phenotype. They examined whether symB and symC, the E. festucae homologs of Podospora anserina self-signaling genes IDC2 and IDC3, are required for E. festucae hyphal fusion and host symbiosis. Deletion mutants of these genes were defective in hyphal cell fusion, formed intra-hyphal hyphae, and had enhanced conidiation. SymB-GFP and SymC-mRFP1 localize to plasma membrane, septa and points of hyphal cell fusion. Plants infected with ΔsymB and ΔsymC strains were severely stunted. Hyphae of the mutants colonized vascular bundles, were more abundant than wild type in the intercellular spaces and formed intra-hyphal hyphae. Although these phenotypes are identical to those previously observed for cell wall integrity MAP kinase mutants no difference was observed in the basal level of MpkA phosphorylation or its cellular localization in the mutant backgrounds. Both genes contain binding sites for the transcription factor ProA. Collectively these results show that SymB and SymC are key components of a conserved signaling network for E. festucae to maintain a mutualistic symbiotic interaction within L. perenne. © 2016 The Authors. Molecular Microbiology Published by John Wiley & Sons Ltd.

  14. Productividad y calidad del huevo de gallinas con niveles crecientes de harina de semilla de calabaza (Cucurbita maxima

    Directory of Open Access Journals (Sweden)

    Yordan Martínez Aguilar

    2012-01-01

    Full Text Available Un total de 160 gallinas ponedoras White Leghorn (Híbrido L-33 en pleno pico de potura, se ubicaron durante 91 días según diseño completamente aleatorizado, para evaluar el comportamiento productivo y la calidad del huevo. Se utilizaron cuatro tratamientos 0, 33, 66 y 100 g/kg de harina de semilla de calabaza, sustituyendo totalmente el aceite vegetal y parcial la torta de soya. Se tuvieron 20 repeticiones por tratamiento. No se evidenció morbilidad y mortalidad en las gallinas ponedoras con la inclusión de la semilla en las dietas. Además no se detectaron diferencias significativas (P>0.05 entre tratamientos para la producción de huevos, viabilidad, consumo de energía metabolizable y nutrientes (excepto para fibra cruda y extracto etéreo, peso del huevo, altura de la clara densa, altura de la yema, grosor de la cáscara, huevos no aptos y calidad sensorial del huevo (sabor, olor y textura. Para los tratamientos con 66 y 100 g/kg de harina de semilla de calabaza se observó un aumento en el peso final de las gallinas en 61 y 66 g, respectivamente en comparación con el tratamiento testigo. Los resultados obtenidos permiten recomendar la inclusión de hasta 100 g/kg de harina de semilla de calabaza en las dietas de gallinas ponedoras, sin alterar el comportamiento productivo y la calidad del huevo.

  15. Effects of inclusion of graded amounts of soya bean hulls on feed intake, chewing activity and nutrient digestibility in dairy cows.

    Science.gov (United States)

    Mohammadzadeh, H; Rezayazdi, K; Nikkhah, A

    2014-06-01

    Twelve multiparous Holstein dairy cows were used in a replicated 4 × 4 Latin square changeover design to evaluate the effects of graded inclusion of soya bean hulls (SHs) in replacement of diet forages at 0%, 10%, 20% and 30% of dietary dry matter (DM) basis on peNDF > 8 and peNDF > 1.18 contents of diets and their resulting effects on chewing activity, nutrient digestibility and milk production of dairy cattle. The control diet contained 50% forage, 50% concentrate and no SH. In the other three diets, SH was substituted for alfalfa hay, corn silage and wheat bran to supply 10%, 20% and 30% of the dietary DM. Increasing SH concentration in the diets resulted in decreasing concentrations of forage neutral detergent fibre (NDF), physically effective NDF (peNDF) and mean particle size (p 1.18 when SH was included in the diets (p 8 among the diets and that these differences are better reflected in terms of DMI, chewing activity and nutrient digestibility, but not in rumen pH. Journal of Animal Physiology and Animal Nutrition © 2013 Blackwell Verlag GmbH.

  16. Assessment of strobilurin fungicides' content in soya-based drinks by liquid micro-extraction and liquid chromatography with tandem mass spectrometry.

    Science.gov (United States)

    Campillo, Natalia; Iniesta, María Jesús; Viñas, Pilar; Hernández-Córdoba, Manuel

    2015-01-01

    Seven strobilurin fungicides were pre-concentrated from soya-based drinks using dispersive liquid-liquid micro-extraction (DLLME) with a prior protein precipitation step in acid medium. The enriched phase was analysed by liquid chromatography (LC) with dual detection, using diode array detection (DAD) and electrospray-ion trap tandem mass spectrometry (ESI-IT-MS/MS). After selecting 1-undecanol and methanol as the extractant and disperser solvents, respectively, for DLLME, the Taguchi experimental method, an orthogonal array design, was applied to select the optimal solvent volumes and salt concentration in the aqueous phase. The matrix effect was evaluated and quantification was carried out using external aqueous calibration for DAD and matrix-matched calibration method for MS/MS. Detection limits in the 4-130 and 0.8-4.5 ng g(-1) ranges were obtained for DAD and MS/MS, respectively. The DLLME-LC-DAD-MS method was applied to the analysis of 10 different samples, none of which was found to contain residues of the studied fungicides.

  17. N- and S-doped high surface area carbon derived from soya chunks as scalable and efficient electrocatalysts for oxygen reduction

    Science.gov (United States)

    Rana, Moumita; Arora, Gunjan; Gautam, Ujjal K.

    2015-02-01

    Highly stable, cost-effective electrocatalysts facilitating oxygen reduction are crucial for the commercialization of membrane-based fuel cell and battery technologies. Herein, we demonstrate that protein-rich soya chunks with a high content of N, S and P atoms are an excellent precursor for heteroatom-doped highly graphitized carbon materials. The materials are nanoporous, with a surface area exceeding 1000 m2 g-1, and they are tunable in doping quantities. These materials exhibit highly efficient catalytic performance toward oxygen reduction reaction (ORR) with an onset potential of -0.045 V and a half-wave potential of -0.211 V (versus a saturated calomel electrode) in a basic medium, which is comparable to commercial Pt catalysts and is better than other recently developed metal-free carbon-based catalysts. These exhibit complete methanol tolerance and a performance degradation of merely ˜5% as compared to ˜14% for a commercial Pt/C catalyst after continuous use for 3000 s at the highest reduction current. We found that the fraction of graphitic N increases at a higher graphitization temperature, leading to the near complete reduction of oxygen. It is believed that due to the easy availability of the precursor and the possibility of genetic engineering to homogeneously control the heteroatom distribution, the synthetic strategy is easily scalable, with further improvement in performance.

  18. Factors affecting the acceptability and consumption of Corn Soya Blend Plus as a prenatal dietary supplement among pregnant women in rural Cambodia.

    Science.gov (United States)

    Janmohamed, Amynah; Karakochuk, Crystal D; Boungnasiri, Somchit; Whitfield, Kyly C; Chapman, Gwen E; Janssen, Patricia; McLean, Judy; Green, Timothy J

    2016-07-01

    Undernutrition is prevalent among pregnant women in Cambodia. The provision of fortified dietary supplements is one strategy to help pregnant women meet their nutritional needs. Corn Soya Blend Plus (CSBP) is a widely used prenatal dietary supplement in areas with high rates of undernutrition and food insecurity. However, little is known about its acceptability during pregnancy. The present study aimed to identify factors that affected the acceptability and consumption of CSBP supplements among pregnant women. Women completed a structured interview designed to provide information on facilitators of and barriers to utilization. In addition, six focus groups were conducted with a subset of women (n 70) to further explore attitudes, perceptions and experiences related to CSBP use. Two districts in Kampong Chhnang Province, Cambodia. Pregnant women (n 288) participating in a cluster-randomized trial of CSBP. The acceptability of CSBP was influenced by sensory attributes, family support, peer influences, and attitudes related to diet, nutritional status and weight gain in pregnancy. Attaining adequate nutrition was considered less important than other concerns during pregnancy, particularly anxiety related to the costs of delivery and postpartum care. Acceptance was lower among new mothers due to fears of weight gain. Health benefits were common reasons for continued use and minor side-effects, such as nausea, were not major barriers to consumption. CSBP was generally well accepted in this population. However, organoleptic factors and perceptions regarding nutrition and weight gain in pregnancy, particularly for first-time mothers, were barriers to increasing acceptance among Cambodian women.

  19. Social mobilization and social marketing to promote NaFeEDTA-fortified soya sauce in an iron-deficient population through a public-private partnership.

    Science.gov (United States)

    Wang, Bo; Zhan, Siyan; Sun, Jing; Lee, Liming

    2009-10-01

    The present pilot project aimed to assess the effectiveness of social mobilization and social marketing in improving knowledge, attitudes and practices (KAP) and Fe status in an Fe-deficient population. In an uncontrolled, before-after, community-based study, social mobilization and social marketing strategies were applied. The main outcomes included KAP and Hb level and were measured at baseline, 1 year later and 2 years later. One urban county and two rural counties in Shijiazhuang Municipality, Hebei Province, China. Adult women older than 20 years of age and young children aged from 3 to 7 years were selected from three counties to attend the evaluation protocol. After 1 year, most knowledge and attitudes had changed positively towards the prevention and control of anaemia. The percentage of women who had adopted NaFeEDTA-fortified soya sauce increased from 8.9% to 36.6% (P children. Social mobilization and social marketing activities had a positive impact on the KAP of adult women, and resulted in marked improvements in Hb levels in both adult women and young children. This should be recommended as a national preventive strategy to prevent and control Fe deficiency and Fe-deficiency anaemia.

  20. Seleccion de cepas de rizobios en suelos promisorios para el cultivo de la soya (Glycine max (L Merril en Colombia

    Directory of Open Access Journals (Sweden)

    Sylvester Bradley Rosemary

    1989-06-01

    Full Text Available En el invernadero se evaluó la efectividad de las cepas preseleccionadas de B. japonicum (CIAT 199,209,3778,3874 y 3876 de la recomendada (CIAT51 y una de Rhizobium spp (CIAT 3779. En el suelo Fluventic Haplustoll, de reacción casi neutra, se presentó respuesta altamente significativa a la inoculación. El tratamiento fertilizado con 150 kg de N/ha produjo el mayor rendimiento de N. Las cepas más efectivas fueron CIAT 3778 y 51, que también presentaron el mayor indice de efectividad a la inoculación (IEI. En el suelo Typic Pellustert,de reacción alcalina, se presentó respuesta significativa a la inoculación. El mayor rendimiento de N se obtuvo con las cepas CIAT 3778 y 51, que fueron además estadísticamente iguales a las cepas CIAT 199 y 3876 en cuanto al IEI. En el suelo Arenic Haplustalf, de reacción ligeramente ácida, hubo respuesta altamente significativa a la inoculación y la cepa más efectiva fue la CJAT 199. En el suelo Fluventic Ustropept, de reacci6n ligeramente ácida, se presentaron diferencias significativas entre los tratamientos inoculados. El mayor rendimiento de N se presentó en el tratamiento fertilizado con N. Las mejores cepas fueron CIAT 3778,3779 y 209. En el suelo Vertic Ustropept, de reacción alcalina, se presentó respuesta altamente significativa a la inoculación. En cuanto al rendimiento de N no se encontraron diferencias entre el tratamiento fertilizado con N y las cepas CIAT 51, 209 y CIAT 3778, siendo también las más efectivas en cuanto al IEI, junto oon la cepa CIAT 199. En los suelos Tropentic Haplorthox y Typic Tropudult no se enoontró respuesta a la inoculación.Screening of Rhizobium strains in soils apt for soybean (Glycine max (L Merril cultivation in Colombia. Under greenhouse conditions were evaluated the effectivity of the preselected strains of the preselected strains of B. japonicum (CIAT 199, 209, 3778, 3874 and 3876, in addition to the recommended strain (CIAT 51 and one of

  1. Tratamiento con implantes Leader-Nano en paciente con oligodoncia

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    Salvador Javier Santos Medina

    2015-03-01

    Full Text Available Los implantes dentales de titanio han revolucionado el mundo de la rehabilitación desde su surgimiento. De manera particular, el empleo de implantes de carga inmediata acorta el tiempo quirúrgico y protésico, con el consiguiente bienestar estético. Se presenta el caso de una paciente femenina de 32 años de edad, con antecedentes de oligodoncia de ambos incisivos laterales superiores y portadora de prótesis parcial acrílica. Fue atendida por el equipo multidisciplinario de implantes en la Clínica Estomatológica Docente “3 de Octubre” y se le realizó tratamiento de rehabilitación integral con implantes Leader-Nano y prótesis fija con corona acrílica sobre dichos implantes. La implantología fue satisfactoria en la paciente; la mejoría estética y funcional, así como la satisfacción de la paciente, fueron los principales logros obtenidos

  2. con dietas suplementadas con Cromo-L-metionina

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    Ram\\u00F3n Garc\\u00EDa-Castillo

    2006-01-01

    Full Text Available Un total de 48 cerdos (Sus scrofa domesticus; 24 machos castrados y 24 hembras cruzados (Yorkshire, Hampshire, Duroc y Landrace de 3,5 a 4,0 meses de edad y 60,0 ± 5,0 kg PV en finalización. Se alimentaron con dietas isoproteícas (14,5 % PC e isoenergéticas (3.400 kcal EM/kg de MS, adicionadas con Cr-L-metionina (MiCroPlex® (0, 200, 400 y 600 ppb. El experimento tuvo una duración de 45 días y se realizó de agosto a noviembre del 2002 en las instalaciones de la Universidad Autónoma Agraria Antonio Narro, localizada en Saltillo, Coahuila, México. Al tener los animales aproximadamente 95 kg PV, se tomó muestra de 15 ml de sangre por cada animal para determinar la concentración de glucosa, ácido úrico, creatinina, urea, proteinas totales y colesterol. Se aplicó un diseño completamente al azar con arreglo factorial 2 x 4; dos para el factor sexo y cuatro para nivel de cromo. Los metabolitos en suero no fueron afectados (P>0,05 por el factor sexo. La glucosa en suero disminuyó (P<0,05 y el colesterol incrementó (P<0,05 con cromo en la dieta. Se concluye que el Cr incrementa el metabolismo de glucosa y disminuye el de colesterol, con lo cual puede haber energía disponible para síntesis de proteína la cual es necesaria para el crecimiento de los animales

  3. Recubrimiento de acero con polidopamina

    OpenAIRE

    Carrasco Rodríguez, Javier

    2013-01-01

    Se ha obtenido recubrimientos de polidopamina en acero mecánicamente resistentes y con tiempos de obtención relativamente pequeños a través de la polimerización de la dopamina bajo diferentes condiciones.

  4. Determination of biogenic amines in fermented product from soya beans by high performance liquid chromatography%高效液相色谱法测定大豆发酵制品中的生物胺

    Institute of Scientific and Technical Information of China (English)

    王充; 易少凌

    2015-01-01

    目的:从生物胺角度初步探讨大豆发酵制品的食用安全性,为确定大豆发酵制品的推荐安全摄入水平提供初步参考。方法采用柱前衍生高效液相色谱法检测大豆发酵制品中生物胺的含量,以0.4 mol/L高氯酸为提取液,以丹酰氯为衍生试剂,紫外检测波长254 nm。结果样品组分分离及测定结果重现性较好,豆豉、豆酱、酱油3种大豆发酵制品均有部分品牌组胺含量超过危害作用水平(即500 mg/kg),8个样品中有7种超过100 mg/kg,同类不同品牌大豆发酵制品间组胺含量没有明显差别;色胺均未检出;除组胺外,3种大豆发酵制品中尚可能存在2-苯乙胺、腐胺及尸胺。结论大豆发酵制品的色胺未检出,组胺含量较高,大量食用可能影响人体健康。%ABSTRACT:Objective To assess the security of fermented product from soya beans, and give a reference for their reference intakes. Methods To determine biogenic amines in lobster sauce, soy sauce and soybean paste by high performance liquid chromatography (HPLC), the biogenic amines were extracted with 0.4 mol/L perchloric acid solution, the pre-column derivatising agent was dansyl chloride, the mobile phase was acetonitrile and water (V:V=60:40), and the flow rate was 1.0 mL/min. UV detector was used to determine the biogenic amines, and the wavelength was 254 nm. Results Histamine was found in every kind of the fermented product with soya beans, and the concentration was higher than 500 mg/kg, which was the level in food that would cause harm to human body made by the Food and Drug Administration (FDA) in America. The average concentration of histamine in the fermented product with soya beans determined was 469.19 mg/kg, and different kinds of fermented products with soya beans had almost the same concentration of histamine. While no tryptamine was found in these samples, there were 2-phenylethylamine, putrescine and cadaverine in these fermented product from

  5. Sistemas de custeio para firmas agroalimentares: o caso dos laticínios e empresas processadoras de soja no Brasil Cost management systems for agrifood firms: the case of the dairy industry and soya bean industry

    Directory of Open Access Journals (Sweden)

    Fernando Cezar Leandro Scramim

    1998-08-01

    Full Text Available Este artigo analisa a aplicação de sistemas de custeio pelas indústrias processadoras de soja e laticínios no Brasil. O objetivo do trabalho foi verificar se a estrutura do setor, ao influenciar a estratégia competitiva a ser adotada pelas organizações, influenciaria o sistema de apuração e controle de custos utilizado nas empresas estudadas. A metodologia utilizada foi a entrevista pessoal junto a 06 (seis esmagadoras de soja e 08 (oito laticínios. No setor lácteo, os resultados apontam para um estado bastante precário relativo à utilização de sistemas de gerenciamento de custos. Por outro lado, no setor de oleaginosas verificou-se uma maior preocupação com a utilização de sistemas de controle mais rigorosos dos custos industriais, evidenciado pela utilização intensiva de sistemas de custeio mais tradicionais.This paper analyses the application of cost management systems in the Brazilian soya bean and dairy industries. The aim of this study was to evaluate whether the structure of the sector, which influences the competitive strategy of the organizations, would influence the cost management system that the firms were using. The methodology was based on personal interviews in 06 (six soya bean companies and 08 (eight dairy companies. In the dairy sector the results indicate a precarious situation related to the application of the cost management systems. On the other hand, in the soya bean sector the results show a greater concern with the use of more rigorous control systems of the industrial costs. This was demonstrated by the intensive use of more traditional cost management systems by the companies studied.

  6. Toxicidad en peces de herbicidas formulados con glifosato Toxicity in fishes of herbicides formulated with glyphosate

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    Maria Alvarez

    2012-07-01

    Full Text Available En nuestro país existe una gran extensión de hectáreas cultivadas con soja transgénica, la misma ha sido modificada genéticamente para soportar la acción de un herbicida denominado glifosato. Debido a la gran cantidad de formulaciones comerciales que incluyen glifosato es de importancia analizar el impacto ambiental producido por éstas. La evaluacion de la toxicidad aguda de dos herbicidas comerciales formulados con glifosato y de una solución del mismo; frente a peces de la especie Poecilia reticulata "lebistes" acusa que una de las soluciones produce mortalidad del 100 % de los especimenes a 100 μl/l (equivalente a 48 mg/l de principio activo; la otra a 50 μl/l (equivalente a 24 mg/l de principio activo y la solución formulada con glifosato puro no produce mortalidad aún a concentraciones de 400 mg/l. Utilizando dosis sub letales en función de los datos obtenidos en el ensayo de toxicidad aguda se determinó que a largo plazo especimenes de Cyprinus carpio haematopterus "carpa koi", manifestaron severas alteraciones hematológicas principalmente frente a una de las formulaciones evaluadas.Nowadays, transgenic soya, modified in order to withstand the impact of the herbicide glyphosate, in one of the main crops grown in Argentina. Due to the large number of commercial formulations that include this drug, is important to analyze both, the acute and chronic environmental impact that they cause. Here the acute toxicity of two commercial herbicides glyphosatebased toward the fish Poecilia reticulate "guppy" was evaluated and compared with pure glyphosate solutions. Interestingly, while commercial herbicides formulations induce a 100% of mortality at concentration ranged between 50 and 100 μl/l, the pure glyphosathe does not present mortality even at doses higher than 400 mg/l. When some long term effects toward Cyprinus carpio haematopterus "koi" were determined by using the sub-lethal doses already calculated it was demonstrated that

  7. CARACTERIZACIÓN QUÍMICA DEL GRANO MOLIDO Y LA TORTA DE SOYA DESGRASADA (Glycine max, Merr; VAR. INCA SOY 24) QUE SE EMPLEAN EN EL MEDIO DE CULTIVO PARA Bradyrhizobium elkanii

    OpenAIRE

    Aida T. Rodríguez; María C. Nápoles; M. A. Ramírez; Yamilet Gutiérrez; Daimy Costales

    2004-01-01

    Algunos derivados de la soya se han utilizado como medio de cultivo para Bradyrhizobium, demostrándose su efecto positivo sobre la dinámica de crecimiento de estos microorganismos, así como su poder inductor sobre la expresión de los genes de nodulación. El presente trabajo se llevó a cabo en el Departamento de Fisiología y Bioquímica Vegetal, perteneciente al Instituto Nacional de Ciencias Agrícolas (INCA), donde se realizó la caracterización química del grano molido y la torta desgrasada de...

  8. Santiago, una ciudad con temor

    Directory of Open Access Journals (Sweden)

    Enrique Oviedo S.

    1999-04-01

    Full Text Available El objetivo general de este artículo es evaluar los efectos de la inseguridad ciudadana en el uso del espacio público. Dicha evaluación exige analizar dos relaciones que se establecen en el ámbito de la violencia: la relación entre victimización y percepción de inseguridad; y la que se establece entre actitudes sociales y resolución pacífica de conflictos nacionales. Para ello, se analizaron las variables victimización, percepción de inseguridad, uso del espacio físico, actitudes hacia el sistema institucional político y social y hacia la resolución de conflictos nacionales, y las posibles relaciones entre ellas. Los datos para realizar el estudio se obtuvieron por medio de una encuesta que se llevó a cabo con 1 200 personas de 18 y 70 años de edad residentes en la ciudad de Santiago. Los resultados indican que Santiago es una ciudad de habitantes con temor y que el aumento de la percepción de inseguridad de sus habitantes contrasta con el hecho de que las tasas de victimización se hayan mantenido, más o menos, constantes en los años que precedieron a la encuesta. El temor se relaciona con el abandono del espacio público físico y sociopolítico, así como con el refugio en los espacios y la vida privados. La actitud de resolver los conflictos por medios no pacíficos es frecuente y se asocia en mayor medida con la inseguridad, la actitud negativa hacia la democracia y la falta de expectativas sobre el futuro del país. Los resultados de este estudio respaldan la idea de que para superar el temor la gente tiende a adaptarse a la realidad adoptando una postura conformista, homogeneizando las creencias y los comportamientos, y sobreestimando la fuerza como medio para resolver las diferencias.

  9. Santiago, una ciudad con temor

    Directory of Open Access Journals (Sweden)

    Oviedo S. Enrique

    1999-01-01

    Full Text Available El objetivo general de este artículo es evaluar los efectos de la inseguridad ciudadana en el uso del espacio público. Dicha evaluación exige analizar dos relaciones que se establecen en el ámbito de la violencia: la relación entre victimización y percepción de inseguridad; y la que se establece entre actitudes sociales y resolución pacífica de conflictos nacionales. Para ello, se analizaron las variables victimización, percepción de inseguridad, uso del espacio físico, actitudes hacia el sistema institucional político y social y hacia la resolución de conflictos nacionales, y las posibles relaciones entre ellas. Los datos para realizar el estudio se obtuvieron por medio de una encuesta que se llevó a cabo con 1 200 personas de 18 y 70 años de edad residentes en la ciudad de Santiago. Los resultados indican que Santiago es una ciudad de habitantes con temor y que el aumento de la percepción de inseguridad de sus habitantes contrasta con el hecho de que las tasas de victimización se hayan mantenido, más o menos, constantes en los años que precedieron a la encuesta. El temor se relaciona con el abandono del espacio público físico y sociopolítico, así como con el refugio en los espacios y la vida privados. La actitud de resolver los conflictos por medios no pacíficos es frecuente y se asocia en mayor medida con la inseguridad, la actitud negativa hacia la democracia y la falta de expectativas sobre el futuro del país. Los resultados de este estudio respaldan la idea de que para superar el temor la gente tiende a adaptarse a la realidad adoptando una postura conformista, homogeneizando las creencias y los comportamientos, y sobreestimando la fuerza como medio para resolver las diferencias.

  10. Exogenous melatonin suppresses dark-induced leaf senescence by activating the superoxide dismutase-catalase antioxidant pathway and down-regulating chlorophyll degradation in excised leaves of perennial ryegrass (Lolium perenne L.

    Directory of Open Access Journals (Sweden)

    Jing Zhang

    2016-10-01

    Full Text Available Leaf senescence is a typical symptom in plants exposed to dark and may be regulated by plant growth regulators. The objective of this study was to determine whether exogenous application of melatonin (N-acetyl-5-methoxytryptamine suppresses dark-induced leaf senescence and the effects of melatonin on reactive oxygen species (ROS scavenging system and chlorophyll degradation pathway in perennial grass species. Mature perennial ryegrass (Lolium perenne L. cv. ‘Pinnacle’ leaves were excised and incubated in 3 mM 2-(N-morpholino ethanesulfonic buffer (pH 5.8 supplemented with melatonin or water (control and exposed to dark treatment for 8 d. Leaves treated with melatonin maintained significantly higher endogenous melatonin level, chlorophyll content, photochemical efficiency, and cell membrane stability expressed by lower electrolyte leakage and malondialdehyde (MDA content compared to the control. Exogenous melatonin treatment also reduced the transcript level of chlorophyll degradation-associated genes and senescence marker genes (LpSAG12.1, Lph36, and Lpl69 during the dark treatment. The endogenous O2- production rate and H2O2 content were significantly lower in these excised leaves treated with melatonin compared to the water control. Exogenous melatonin treatment caused increases in enzymatic activity and transcript levels of superoxide dismutase and catalase but had no significant effects on ascorbate peroxidase, glutathione reductase, dehydroascorbate reductase, and monohydroascorbate reductase. The content of non-enzymatic antioxidants, such as ascorbate and dehydroascorbate, were decreased by melatonin treatment, while the content of glutathione and oxidized glutathione was not affected by melatonin. These results suggest that the suppression of dark-induced leaf senescence by exogenous melatonin may be associated with its roles in regulating ROS scavenging through activating the superoxide dismutase-catalase enzymatic antioxidant

  11. Recovery rate of children with moderate acute malnutrition treated with ready-to-use supplementary food (RUSF) or improved corn-soya blend (CSB+): a randomized controlled trial.

    Science.gov (United States)

    Medoua, Gabriel Nama; Ntsama, Patricia M; Ndzana, Anne Christine A; Essa'a, Véronique J; Tsafack, Julie Judith T; Dimodi, Henriette T

    2016-02-01

    To compare an improved corn-soya blend (CSB+) with a ready-to-use supplementary food (RUSF) to test the hypothesis that satisfactory recovery rate will be achieved with CSB+ or RUSF when these foods provide 50 % of the child's energy requirement, the 50 % remaining coming from usual diet. A comparative efficacy trial study was conducted with moderately wasted children, using a controlled randomized design, with parallel assignment for RUSF or CSB+. Every child received a daily ration of 167 kJ (40 kcal)/kg body weight during 56 d with a follow-up performed every 14 d. Every caregiver received nutrition counselling at enrolment and at each follow-up visit. Health districts of Mvog-Beti and Evodoula in the Centre region of Cameroon. Eight hundred and thirty-three children aged 6-59 months were screened and eighty-one malnourished children (weight-for-height Z-score between -3 and -2) aged 25-59 months were selected. Of children treated with CSB+ and RUSF, 73 % (95 % CI 59 %, 87 %) and 85 % (95 % CI 73 %, 97 %), respectively, recovered from moderate acute malnutrition, with no significant difference between groups. The mean duration of treatment required to achieve recovery was 44 d in the RUSF group and 51 d in the CSB+ group (log-rank test, P=0·0048). There was no significant difference in recovery rate between the groups. Both CSB+ and RUSF were relatively successful for the treatment of moderate acute malnutrition in children. Despite the relatively low ration size provided, the recovery rates observed for both groups were comparable to or higher than those reported in previous studies, a probable effect of nutrition education.

  12. Effect of super dosing of phytase on growth performance, ileal digestibility and bone characteristics in broilers fed corn-soya-based diets.

    Science.gov (United States)

    Manobhavan, M; Elangovan, A V; Sridhar, M; Shet, D; Ajith, S; Pal, D T; Gowda, N K S

    2016-02-01

    A feeding trial was designed to assess the effect of super dosing of phytase in corn-soya-based diets of broiler chicken. One hundred and sixty-eight day-old broilers were selected and randomly allocated to four dietary treatment groups, with 6 replicates having 7 chicks per treatment group. Two-phased diets were used. The starter and finisher diet was fed from 0 to 3 weeks and 4 to 5 weeks of age respectively. The dietary treatments were consisted of normal phosphorus (NP) group without any phytase enzyme (4.5 g/kg available/non-phytin phosphorus (P) during starter and 4.0 g/kg during finisher phase), three low-phosphorus (LP) groups (3.2 g/kg available/non-phytin P during starter and 2.8 g/kg during finisher phase) supplemented with phytase at 500, 2500, 5000 FTU/kg diet, respectively, to full fill their phosphorus requirements. The results showed that super doses of phytase (at 2500 FTU and 5000 FTU/kg) on low-phosphorus diet improved feed intake, body weight gain, ileal digestibility (serine, aspartic acid, calcium, phosphorus), blood P levels and bone minerals such as calcium (Ca), P, magnesium (Mg) and zinc (Zn) content. It could be concluded that super doses of phytase in low-phosphorus diet were beneficial than the normal standard dose (at 500 FTU/kg) of phytase in diet of broiler chicken. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  13. Ability of Lactobacillus fermentum to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides

    Directory of Open Access Journals (Sweden)

    Sesma Fernando

    2008-01-01

    Full Text Available Abstract Background Soya and its derivatives represent nutritionally high quality food products whose major drawback is their high content of α-galacto-oligosaccharides. These are not digested in the small intestine due to the natural absence of tissular α-galactosidase in mammals. The passage of these carbohydrates to the large intestine makes them available for fermentation by gas-producing bacteria leading to intestinal flatulence. The aim of the work reported here was to assess the ability of α-galactosidase-producing lactobacilli to improve the digestibility of α-galacto-oligosaccharides in situ. Results Gnotobiotic rats were orally fed with soy milk and placed in respiratory chambers designed to monitor fermentative gas excretion. The validity of the animal model was first checked using gnotobiotic rats monoassociated with a Clostridium butyricum hydrogen (H2-producing strain. Ingestion of native soy milk by these rats caused significant H2 emission while ingestion of α-galacto-oligosaccharide-free soy milk did not, thus validating the experimental system. When native soy milk was fermented using the α-galactosidase-producing Lactobacillus fermentum CRL722 strain, the resulting product failed to induce H2 emission in rats thus validating the bacterial model. When L. fermentum CRL722 was coadministered with native soy milk, a significant reduction (50 %, P = 0.019 in H2 emission was observed, showing that α-galactosidase from L. fermentum CRL722 remained active in situ, in the gastrointestinal tract of rats monoassociated with C. butyricum. In human-microbiota associated rats, L. fermentum CRL722 also induced a significant reduction of H2 emission (70 %, P = 0.004. Conclusion These results strongly suggest that L. fermentum α-galactosidase is able to partially alleviate α-galactosidase deficiency in rats. This offers interesting perspectives in various applications in which lactic acid bacteria could be used as a vector for

  14. pacientes con insuficiencia renal terminal

    Directory of Open Access Journals (Sweden)

    Karen Herrera Herrera

    2011-01-01

    Full Text Available La presente investigación fundamenta en la clínica psicoanalítica el estudio de dos casos de tres personas diagnosticadas con IRT que reciben tratamiento de hemodiálisis, en razón a que dadas las características y el aumento de los reportes que se presentan, ya esto se considera un problema de salud pública. El objetivo principal es describir las características dinámicas del proceso de duelo en pacientes con IRT en un centro de terapia renal de la ciudad de Cartagena. El procedimiento metodológico empleó un diseño de tipo cualitativo; la investigación se desarrolló con un diseño clínico mediante el estudio de casos, y fundamentada en la hermenéutica psicoanalítica. Todo esto respaldado en la historia clínica, la entrevista semiestructurada individual y familiar, los test proyectivos, test del dibujo de la figura humana Machover y TAT de Murray, para la debida integración de los análisis. Se concluye que predominan funciones fallidas de los progenitores y que son individuos provenientes de familias psicosomáticas, que utilizan la enfermedad para obtener un beneficio secundario.

  15. con bajo peso al nacer

    Directory of Open Access Journals (Sweden)

    Adriana Mora Antó

    2005-01-01

    Full Text Available Esta investigación dio cuenta de la relación entre el estilo de funcionamiento familiar, los patrones de crianza y las edades de desarrollo evolutivo en niños, nacidos con bajo peso. El estudio descriptivo correlacional se realizó con 41 niños y sus madres, aplicándose cuestionarios sobre funcionamiento familiar, prácticas de crianza y desarrollo infantil. Los resultados señalaron la existencia de un funcionamiento familiar caracterizado por una cohesión amalgamada y una adaptabilidad caótica, una disciplina complaciente, falta de control y de límites claros en la díada madre-hijo. Se trataba de familias monoparentales, donde la temprana edad de concepción, el madresolterismo y el apoyo de la familia extensa eran constantes. Las edades evolutivas registradas indicaron un desarrollo inferior a la edad cronológica, en la mayor parte de los casos; sin embargo, éstas tendieron a ser superiores al compararlas con la edades reales de los infantes. No se encontró una correlación estadísticamente significativa entre la edad de desarrollo y los diferentes factores del funcionamiento familiar para algunos de los rangos de edad considerados; sin embargo, no se lo descartó por completo, especialmente en lo referente al optimismo familiar

  16. Prueba cutánea con extractos alergénicos de pólenes y relación con signos clínicos de rinitis alérgica y asma bronquial en Camagüey, Cuba

    Directory of Open Access Journals (Sweden)

    Olimpio Rodríguez

    2013-08-01

    Full Text Available Los pólenes inciden sobre las mucosas provocando sensibilización alérgica, sin embargo, en Cuba son insuficientes los estudios sobre estos alergenos. Con el objetivo de conocer el comportamiento de Ambrosía psilostachya, Amaranthus leucocarpus, Helianthus annuus, Rumex acetosella, Lolium perenne, Cupressus lindley y Cocos nucifera, se realizó un estudio observacional analítico en el Servicio de Alergia del policlínico Previsora, Camagüey. La muestra fue de 529 individuos con rinitis o asma, con pruebas cutáneas por punción (prick test positivas a los ácaros, hongos y pólenes; el 40% de la misma estaba sensibilizada a pólenes con prueba de provocación nasal a estos alergenos. Se compararon según gravedad de los síntomas y los valores del habón de 3, 4 y 5 mm. Los grupos etarios con valores más altos de sensibilización fueron los de 6 a 12 y 19 a 44 años. No hubo diferencias significativas de sensibilización a los pólenes según sexo. Ambrosía y Lolium presentaron los porcentajes más altos de respuesta cutánea, con habones de 3 mm en un 49,98% y 40,09%, respectivamente. Tampoco se detectaron diferencias significativas. El 89,6% de los pacientes sensibilizados reaccionaron clínicamente a la provocación nasal. En los asintomáticos predominaron los habones de 3 mm y en los graves los de 5 mm. Los pólenes constituyeron una causa importante de sensibilización alérgica. Existió relación entre las pruebas cutáneas y la prueba de provocación nasal. El diámetro del habón de la respuesta cutánea a prick test se relacionó con la severidad de los síntomas clínicos.

  17. Adsorption isotherms, kinetics and column operations for the removal of hazardous dye, Tartrazine from aqueous solutions using waste materials-Bottom Ash and De-Oiled Soya, as adsorbents

    International Nuclear Information System (INIS)

    Mittal, Alok; Mittal, Jyoti; Kurup, Lisha

    2006-01-01

    Adsorbents, Bottom Ash (a power plant waste) and De-Oiled Soya (an agricultural waste) exhibit good efficacy to adsorb a highly toxic dye, Tartrazine. Through the batch technique equilibrium uptake of the dye is observed at different concentrations, pH of the solution, dosage of adsorbents and sieve size of adsorbents. Langmuir and Freundlich adsorption isotherms are successfully employed on both the adsorbents and on the basis of these models the thermodynamic parameters are evaluated. Kinetic investigations reveal that more than 50% adsorption of dye is achieved in about 1 h in both the cases, whereas, equilibrium establishment takes about 3-4 h. The linear plots obtained in rate constant and mass transfer studies further confirm the applicability of first order rate expression and mass transfer model, respectively. The kinetic data treated to identify rate controlling step of the ongoing adsorption processes indicate that for both the systems, particle diffusion process is predominant at higher concentrations, while film diffusion takes place at lower concentrations. The column studies reveal that about 96% saturation of both the columns is attained during their exhaustion, while about 88 and 84% of the dye material is recovered by eluting dilute NaOH solution through exhausted Bottom Ash and De-Oiled Soya columns, respectively

  18. Adsorption isotherms, kinetics and column operations for the removal of hazardous dye, Tartrazine from aqueous solutions using waste materials-Bottom Ash and De-Oiled Soya, as adsorbents

    Energy Technology Data Exchange (ETDEWEB)

    Mittal, Alok [Department of Applied Chemistry, Maulana Azad National Institute of Technology, Bhopal 462 007 (India)]. E-mail: aljymittal@yahoo.co.in; Mittal, Jyoti [Department of Applied Chemistry, Maulana Azad National Institute of Technology, Bhopal 462 007 (India); Kurup, Lisha [Department of Applied Chemistry, Maulana Azad National Institute of Technology, Bhopal 462 007 (India)

    2006-08-25

    Adsorbents, Bottom Ash (a power plant waste) and De-Oiled Soya (an agricultural waste) exhibit good efficacy to adsorb a highly toxic dye, Tartrazine. Through the batch technique equilibrium uptake of the dye is observed at different concentrations, pH of the solution, dosage of adsorbents and sieve size of adsorbents. Langmuir and Freundlich adsorption isotherms are successfully employed on both the adsorbents and on the basis of these models the thermodynamic parameters are evaluated. Kinetic investigations reveal that more than 50% adsorption of dye is achieved in about 1 h in both the cases, whereas, equilibrium establishment takes about 3-4 h. The linear plots obtained in rate constant and mass transfer studies further confirm the applicability of first order rate expression and mass transfer model, respectively. The kinetic data treated to identify rate controlling step of the ongoing adsorption processes indicate that for both the systems, particle diffusion process is predominant at higher concentrations, while film diffusion takes place at lower concentrations. The column studies reveal that about 96% saturation of both the columns is attained during their exhaustion, while about 88 and 84% of the dye material is recovered by eluting dilute NaOH solution through exhausted Bottom Ash and De-Oiled Soya columns, respectively.

  19. Adsorption isotherms, kinetics and column operations for the removal of hazardous dye, Tartrazine from aqueous solutions using waste materials--Bottom Ash and De-Oiled Soya, as adsorbents.

    Science.gov (United States)

    Mittal, Alok; Mittal, Jyoti; Kurup, Lisha

    2006-08-25

    Adsorbents, Bottom Ash (a power plant waste) and De-Oiled Soya (an agricultural waste) exhibit good efficacy to adsorb a highly toxic dye, Tartrazine. Through the batch technique equilibrium uptake of the dye is observed at different concentrations, pH of the solution, dosage of adsorbents and sieve size of adsorbents. Langmuir and Freundlich adsorption isotherms are successfully employed on both the adsorbents and on the basis of these models the thermodynamic parameters are evaluated. Kinetic investigations reveal that more than 50% adsorption of dye is achieved in about 1h in both the cases, whereas, equilibrium establishment takes about 3-4h. The linear plots obtained in rate constant and mass transfer studies further confirm the applicability of first order rate expression and mass transfer model, respectively. The kinetic data treated to identify rate controlling step of the ongoing adsorption processes indicate that for both the systems, particle diffusion process is predominant at higher concentrations, while film diffusion takes place at lower concentrations. The column studies reveal that about 96% saturation of both the columns is attained during their exhaustion, while about 88 and 84% of the dye material is recovered by eluting dilute NaOH solution through exhausted Bottom Ash and De-Oiled Soya columns, respectively.

  20. Conversando con...Momoyo Kaijima

    OpenAIRE

    Gómez Alonso, Carlos; Álvarez Isidro, Eva; Torres Barchino, Ana

    2017-01-01

    [ES] Momoyo Kaijima es profesora en la Facultad de Arte y Diseño de la Universidad de Tsukuba en la Prefectura de Ibaraki y profesora visitante en la ETH de Zürich, en Royal Academy of Fine Arts, en Rice School of Architecture y en Harvard GSD. A lo largo de los años, Atelier Bow Wow ha colaborado con Krešimir Rogina, arquitecto de Zagreb y socio de la firma internacional Penezic&Rogina, en la realización del Grožnjan International Summer School of Architecture, siendo Rogina el nexo indispen...

  1. Food irradiation - pros and cons

    International Nuclear Information System (INIS)

    1983-01-01

    The use of ionising radiation for food preservation is a much-disputed topic, both among experts and among consumers. Pros and cons of this issue were discussed in detail at the consumers' forum. Professor Dr. Johannes Friedrich Diehl, Director of the Institute for Biochemistry of the Food Research Centre, Karlsruhe, is a well-known supporter of the new method of food preservation; he sees advantages in the radiopreservation of food because, for example, losses due to inedibility are reduced, the danger of salmonellosis is decreased, just as the use of chemicals. He thinks this method to be without danger to health, shown by many years of experience. Opponents to food irradiation like Prof. Dr. Konrad Pfeilsticker, Professor for food science and food chemistry at the Bonn University deem the method to be unnecessary and raise the problem of qualitative changes caused in the food. In the course of the discussions, the pros and cons seemed to balance each other out. (orig./AJ) [de

  2. De paseo con los Bourbaki

    Directory of Open Access Journals (Sweden)

    Miquel Escudero

    2012-04-01

    Full Text Available Setenta y siete años después de la fundación del grupo Bourbaki, procedemos a una reflexión que puede ser útil para los estudiantes que acaso no sepan de su existencia, ni tan siquiera los de matemáticas. Sería interesante conocer que a este grupo se le debe el signo del vacío como conjunto. Con todo lo discutible que sea el método Bourbaki en su reinterpretación de la matemática, no cabe duda de su importante repercusión hasta el punto de que ha marcado una época. Hay un antes y un después tras su irrupción, ningún matemático de primera fila de la segunda mitad del siglo XX fue ajeno a su influjo, para encabezarlo o para reprobarlo. Comenzaron como una juvenil extravagancia, pero repleta de conocimientos y con decidida voluntad de aprehender el rigor de forma exhaustiva.

  3. Tratamiento del paciente con artrosis

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    Francisco Vargas Negrín

    2014-01-01

    Full Text Available El manejo terapéutico del paciente con artrosis tiene como objetivo disminuir la sintomatología dolorosa e inflamatoria, mejorar la capacidad funcional del paciente y la aplicación de intervenciones terapéuticas eficaces y lo más seguras posibles. Un enfoque centrado en el paciente implica su participación activa en el diseño del plan terapéutico y en la toma de decisiones informadas oportunas en todas las etapas de la enfermedad. La educación terapéutica, la actividad física y el ejercicio terapéutico junto con el control de peso, en caso de sobrepeso u obesidad, constituyen el núcleo central del tratamiento. Los autocuidados individuales y por los familiares son fundamentales en el control del día a día del paciente. El uso de terapias físicas, ayudas técnicas (bastón, etc. y de fármacos tipo analgésicos simples, opioides y antiinflamatorios tiene evidencias demostradas en el control del dolor, mejora la funcionalidad y la calidad de vida del paciente y una clara recomendación de uso en el tratamiento de la artrosis. La cirugía conservadora y la de reemplazo articular se indican en los casos en los que no se logran los objetivos terapéuticos en casos concretos.

  4. Towards non-invasive methods to determine the effect of treatment of soya-bean meal on lysine availability in dairy cows.

    Science.gov (United States)

    Borucki Castro, S I; Lapierre, H; Phillip, L E; Jardon, P W; Berthiaume, R

    2008-02-01

    Lysine (Lys) availability in three different soya-bean meal (SBM) products was determined using the following techniques: whole body (WB) net flux of Lys, digestible Lys (duodenal flow × intestinal digestibility) and the plasma Lys response curve method of Rulquin and Kowalczyk (2003). Four multiparous Holstein cows (173 days in milk) were equipped with ruminal and duodenal cannulas and used in a 4 × 4 Latin square experiment with 14-day periods. The animals were fed either solvent-extracted SBM (SE), expeller-processed SBM (EP) or lignosulphonate-treated SBM (LS) at 23% of the diet dry matter (DM). The fourth treatment (SE70) consisted of a continuous infusion of Lys (70 g/day) into the omasum of cows fed the SE diet. Chromium(III) oxide was included as a digesta marker in order to determine the duodenal flow of amino acids (AA). On day 12 of each experimental period, six blood samples were collected to determine plasma Lys concentrations. Immediately after that, a pulse dose of L-[2-15N] Lys was administered in the jugular vein. Jugular blood samples were then collected at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 13, 16, 19, 25 and 31 min after the injection to determine 15N Lys enrichment. On each of days 13 and 14, eight digesta samples were collected and pooled by period. Amongst the diets of SBM (SE, EP, LS), no differences were observed for duodenal Lys flow or digestible Lys. Duodenal flow of microbial N with SE was numerically higher, compared with EP and LS, indicating enhanced duodenal supply of microbial Lys for this diet, and this may have compensated for the additional Lys derived from undegradable protein in rumen-protected SBM products (EP and LS). The use of the plasma response curve method as well as the measurement of WB Lys flux also revealed no differences in Lys availability among the SBM products. The WB flux method resulted in 100% post-ruminal recovery of the Lys infused with diet SE70 compared with the control diet SE, which indicates that the

  5. Cons ICARUS, TIGER and Fascism

    Directory of Open Access Journals (Sweden)

    Janez Vrečko

    2010-12-01

    Full Text Available Like the scientists of their time, Russian artists in the 1920s considered gravity the central problem – a view which points to the close harmony between modern physics and the avant-garde. It was only with the constructivist movement that the Icarus revolution grasped the principles of the “mobile philosophy” (3.651 which was almost at the same time recognised by modern physics as well. The static view of the world became obsolete, space and time were no longer absolute values. It was necessary to transcend Euclidean geometry, shake off the political ʻshackles on one’s hands’ and surrender to Lisicki’s imaginary space, where “At 2000 metres in the air / there is no more perspective” (Integrals 276.  Kosovel’s Icarus project accorded with Tatlin’s, and both of them accorded with the quintessential aims of the constructivist movement. It is no accident that Kosovel wished to name one of his poetry collections The Dream of Icarus. Poems on the Icarus theme, such as “Cons Icarus”, “Evacuation of the Spirit”, “Eh, Hey”, “A Heart in Alcohol” etc. belong to the group of Kosovel’s conses which follow his “mobile philosophy” (3.650 and “letters growing into space” (Int. 282.  The question “Man, do you want up in the air?” (Int. 128 will remain a question until the moment when man is finally ready to transcend the existing boundary and dive “beyond”. Hence Kosovel’s clear-cut contrast between the “green windows of an illuminated / express on a viaduct”, which moves horizontally and is, like a water current, subject to the earth’s gravity, and “the spirit in space”, whose direction of motion is “the perpendicular of the spirit”, atectonicity. “The spirit burns in space”: fire is an element that knows vertical movement alone, the only one of the elements to outgrow and transcend the earth’s gravity, therefore it is associated with another mythological figure important for

  6. Utilización de la proteína dietaría por alevinos de la gamitana, Colossoma macropomum, alimentados con dietas isocalóricas

    Directory of Open Access Journals (Sweden)

    Félix Walter Gutiérrez

    2011-05-01

    Full Text Available Un experimento fue conducido para evaluar los efectos de cinco niveles de proteína (25,27, 29, 31 y 33% sobre el comportamiento productivo de alevinos de la gamitana, Colossoma macropomum Cuvier 1818, alimentados con dietas isocalóricas (2,7 kcal de ED/g. Los parámetros medidos fueron ganancia de peso (GP, conversión alimenticia (CA, proteína retenida (PR, razón de eficiencia proteica (REP y energía retenida (ER. En la preparación de las dietas experimentales se utilizaron como fuentes de proteína harina de anchoveta y harina de torta de soya y como fuentes de energía maíz amarillo duro, subproducto de trigo y aceite de pescado. Se encontraron diferencias significativas (P<0,05 entre tratamientos para los parámetros GP, PR, REP y ER. No se encontraron diferencias significativas para la CA. Los mejores rendimientos fueron obtenidos cuando las gamitanas fueron alimentadas con niveles dietarios de 25, 27 y 33% de proteína cruda. También se encontró que a medida que se elevó el nivel de proteína de la dieta, la REP decreció significativamente. Tomando en cuenta el costo de la proteína en la dieta, se concluye que la alimentación de la gamitana con niveles dietarios de 25 ó 27% de proteína cruda y 2,7 kcal de ED/g, garantizarán su exitoso crecimiento.

  7. Fare astronomia con piccoli telescopi

    CERN Document Server

    Gainer, Michael K

    2007-01-01

    Non sono necessariamente richiesti strumenti mastodontici per produrre risultati scientificamente validi nel campo dell’astronomia. Anche l’astrofilo dotato di un piccolo telescopio, con un diametro di soli 8-9 cm, può contribuire alla scienza del cielo realizzando utili osservazioni del Sole, della Luna, dei pianeti, delle comete, degli asteroidi, delle stelle doppie o variabili, delle nebulose e degli ammassi stellari. Il manuale di M.K. Gainer spiega quale sia la dotazione minima (un piccolo telescopio, un computer, una semplice fotocamera digitale), come utilizzarla, e quali siano le tecniche appropriate da adottare nelle osservazioni. Offre inoltre schemi per interpretare e ridurre i dati raccolti, nonché schede da compilare e da spedire ai centri di raccolta internazionali. Questo libro è il passaporto grazie al quale l’astrofilo può entrare a pieno titolo nel mondo affascinante della scienza astronomica.

  8. Depresion en pacientes con alteraciones del tiroides

    OpenAIRE

    Radanovic-Grguric´, Ljiljana; Filakovic´, Pavo; Barkic´, Jelena; Mandic´, Nikola; Karner, Ivan; Smoje, Juraj

    2003-01-01

    Nuestro estudio fue realizado en un grupo de 53 mujeres con disfunción tiroidea y 28 mujeres con depresión mayor. Empleamos la Escala de la Depresión de Hamilton, la Escala de Autoevaluación de la Depresión de Zung y la Escala sobre la Impresión Clínica Global. Los resultados del estudio demuestran que la mayoría de los pacientes con disfunción tiroidea se mostraron clínicamente significativos en cuanto al trastorno depresivo. Los episodios depresivos son más frecuentes en pacientes con hipot...

  9. Aprende Ajedrez con Rey - Parte 2

    OpenAIRE

    ESTÉVEZ MONTERO, RAÚL; Lloret Mauri, Jaime

    2016-01-01

    Es una pieza audiovisual creada con el objeto de atraer la atención de los niños de muy corta edad con el ajedrez y familiarizarlos con todas sus piezas y movimientos. Es una animación dirigida a un público infantil presentada por dibujos animados en 2D, en la que se ha intentado respetar en todo momento el argot de la comunidad ajedredística. En este video se presenta la segunda parte. Estévez Montero, R.; Lloret Mauri, J. (2016). Aprende Ajedrez con Rey - Parte 2. http://hdl.handle.net/1...

  10. Aprende Ajedrez con Rey - Parte 1

    OpenAIRE

    ESTÉVEZ MONTERO, RAÚL; Lloret Mauri, Jaime

    2016-01-01

    Es una pieza audiovisual creada con el objeto de atraer la atención de los niños de muy corta edad con el ajedrez y familiarizarlos con todas sus piezas y movimientos. Es una animación dirigida a un público infantil presentada por dibujos animados en 2D, en la que se ha intentado respetar en todo momento el argot de la comunidad ajedredística. En este video se presenta la primera parte. Estévez Montero, R.; Lloret Mauri, J. (2016). Aprende Ajedrez con Rey - Parte 1. http://hdl.handle.net/1...

  11. DETERMINACIÓN DE RESIDUOS DE GLIFOSATO Y DE SU METABOLITO ACIDO AMINOMETILFOSFONICO EN AGUAS MEDIANTE CROMATOGRAFIA LIQUIDA DE ALTA EFICIENCIA CON DERIVACION POSCOLUMNA Y DETECCION POR FLUORESCENCIA

    Directory of Open Access Journals (Sweden)

    Hugo A Rodríguez

    2010-11-01

    Full Text Available El glifosato es un herbicida no selectivo,ampliamente utilizado en el mundo para controlar malezas anuales y perennes. Su principal metabolito en suelos y aguas es el ácido aminometilfosfónico (AMPA formado por la acción de microorganismos. Este herbicida se utiliza en Colombia en altas dosis para la erradicación de cultivos ilícitos de coca y amapola, y como madurante en caña de azúcar, constituyendo un problema ambiental y social en el país, y haciéndose necesaria la evaluación de residuos de glifosato y su metabolito en diferentes matrices. En este trabajo se validó una metodología analítica para determinar residuos de glifosato y de su metabolito AMPA en aguas de influencia de algunas regiones colombianas. El procedimiento experimental comprende dos pasos principales: el primero es un paso de limpieza, extracción y concentración en fase sólida; el Segundo corresponde a la separación, identificación y cuantificación de los compuestos mediante Cromatografía Líquida de Alta Eficiencia (CLAE con derivación poscolumna y detección por fluorescencia. Los resultados de la validación muestran que la metodología es específica, selectiva, precisa y robusta en un rango lineal entre 10 y 750 /xg/L, con límites de detección de 0,8 /xg/L y límites de cuantificación de 2 /ig/L para los dos analitos. Las recuperaciones se encuentran en el orden del 73% para glifosato y del 70% para el AMPA. Además, se muestran los resultados de análisis de aguas tomadas en algunas zonas del país donde se aplica glifosato en diferentes dosis con diferentes propósitos, encontrándose residuos del herbicida y de su metabolito en concentraciones por encima de los valores permitidos en aguas potables para plaguicidas de categoría toxicológica IV, caso del glifosato, de acuerdo con la legislación colombiana

  12. Plantas cubanas con efecto antiinflamatorio

    Directory of Open Access Journals (Sweden)

    Ada Ivis Regalado Veloz

    Full Text Available La actividad antiinflamatoria suscita gran interés científico en el área farmacológica, debido a que muchas enfermedades en su evolución cursan por procesos inflamatorios (artritis reumatoide, ateroesclerosis, cáncer, diabetes, gota, asma, dermatitis, trastornos neurodegenerativos y diversas dolencias menores. Las enfermedades inflamatorias constituyen un problema de salud importante, debido a la falta de medicamentos eficaces y seguros para su uso por periodos prolongados. Hoy en día se trabaja en la búsqueda de alternativas de antiinflamatorios más seguros, en el que las plantas medicinales, una de las formas más antiguas de tratamiento, constituyen una elección a considerar. En este trabajo se realizó una revisión bibliográfica, sobre especies de plantas que crecen en Cuba que le reportan propiedades farmacológicas como antinflamatorios. En la revisión de la literatura se utilizó la base de datos Medline (vía PubMed, así como revistas nacionales desde el periodo de 2000 hasta el presente, con las palabras claves "inflamación" y "plantas cubanas antiinflamatorias" o "actividad antiinflamatoria" y "plantas medicinales".

  13. Hospitalidad, con y sin papeles

    Directory of Open Access Journals (Sweden)

    Ana Paula Penchaszadeh

    Full Text Available Resumen El objetivo de este artículo es vincular el trabajo sobre el archivo de Jacques Derrida con la experiencia de la hospitalidad. Se intentará mostrar que, por un lado, se trata siempre de los papeles, de la legitimidad que éstos otorgan o no tanto a nivel filosófico (deseo de poseer los papeles que autoricen tal o cual decisión interpretativa, como a nivel político ("tener papeles" como el principio básico de todo derecho a tener derechos, de todo derecho a la comunidad. Mas también, por otro lado, se intentará pensar aquello que arruina la idea misma de tener o no tener (papeles, la idea de propiedad, aquello que hace imposible fundar una decisión o identidad en última instancia y, por ende, una soberanía, una frontera. La hospitalidad, la llegada inminente del otro, representa un desafío político y ético para la filosofía: pues no se trata de un saber, sino de una experiencia transformando el sustrato del nos-otros, del ser común.

  14. Itinerari Musicali con la Wiild

    Directory of Open Access Journals (Sweden)

    Elisabetta Nanni

    2013-03-01

    Full Text Available La Wiild, acronimo di Wiimote Lavagna Digitale, è uno strumento didattico che utilizza il telecomando della Wii, il famoso gioco della Nintendo, insieme a un software libero, rendendolo così estremamente versatile. Non vincolato a software proprietario, il suo utilizzo è legato alla capacità dell’insegnante di ripartire dalla didattica, dalle risorse selezionate e dall’epistemologia di ogni singola disciplina, trovando così nel proprio contesto un ruolo per le tecnologie. Il contributo presenta ipotesi di lavoro per l’educazione musicale nella scuola secondaria di primo grado che si sviluppano sia attraverso lo studio del rapporto suono/segno con affinità pittoriche e successiva codificazione grafica, sia attraverso un’attività di laboratorio in cui co-costruire percorsi storico-musicali. La Wiild diventerà davvero utile ed efficace nel momento in cui, affiancando le risorse selezionate dal docente, verrà utilizzata senza essere notata, giocando un ruolo di strumento tecnologico «normale e trasparente».

  15. Desenvolvimento de snacks extrusados a partir de misturas de farinha de soja, fécula e farelo de mandioca Development of extruded snacks from blends of soya flour, cassava starch and bran

    Directory of Open Access Journals (Sweden)

    Fernanda Rossi Moretti Trombini

    2013-01-01

    Full Text Available Neste trabalho, objetivou-se avaliar o efeito das condições de extrusão sobre as propriedades físicas de produtos extrusados. Foi utilizado o delineamento central composto rotacional com quatro variáveis independentes (2(4 e a metodologia de superfície de resposta para avaliar os resultados de índice de expansão, volume específico, índice de absorção de água, índice de solubilidade em água e cor, de acordo com as variações de temperatura de extrusão, rotação da rosca, porcentagem de farelo de mandioca e porcentagem de farinha de soja. Os resultados indicam que é possível produzir novos produtos extrusados com boas propriedades físicas a partir de misturas de farinha de soja, fécula e farelo de mandioca.This study aimed to evaluate the effect of the extrusion conditions on physical properties of extruded products. A factorial central composite design (2(4 with four independent variables and the response surface methodology were used to evaluate the results of expansion index, specific volume, water absorption index, water solubility index and color, according to the variations in barrel temperature, screw speed, percentage of cassava bran and percentage of soya flour. The results indicate that is possible to produce new extruded products with good physical properties from blends of soya flour, cassava starch and bran.

  16. OP-16 DIETARY INTERVENTION USING THE LOW FODMAP DIET VERSUS THE "MILK, EGG, WHEAT AND SOYA FREE" DIET FOR TREATMENT OF FUNCTIONAL GUT DISORDERS A SINGLE CENTRE EXPERIENCE.

    Science.gov (United States)

    Keetarut, K; Kiparissi, F; McCartney, S; Murray, C

    2015-10-01

    The adolescent clinic is a tertiary referral clinic including patients with a wide variety of complex gastroenterology conditions predominantly tertiary referrals fromGreat Ormond Street Hospital transition clinic. To assess the benefit of the low FODMAP diet versus the "Milk, egg, wheat and soya" (MEWS) free diet for symptom control in patients with functional gut disorders and/or food allergy from June 2013 to June 2015. A total of 436 patients were seen during this time period for dietetic advice and the age range varied from 13-21 years old with 43terms of diagnosis used. These included the broad categories of inflammatory bowel disease, food allergy, functional gut conditions, congenital gut disorders, autoimmune disorders and oncology conditions. For functional gut disorders/food allergy there were 14 terms used which varied from "Functional gut disorder" to "Irritable bowel syndrome" and also included patients with delayed gastric emptying. For patients with food allergy the terms "multiple food allergy" or EosinophilicOesophagitis or Colitis were used. A total of 40 patients with functional gut disorders were referred for the MEWS or low FODMAP diet. The efficacy of the diet was measured using a symptom scale pre and post dietary intervention assessing if patients symptoms changed from nil/mild/moderate tosignificant. The results indicate whether the presenting predominant symptom e.g., bloating, constipation or abdominal pain improved following the dietary intervention. A total of 29 patients were seen for the "MEWS" free diet.These were 17 functional, 3 food allergy, 6 IBS, 2 EosinophilicOesophagitis, 1 oncology patient. The age ranged from 14 to 21 and average ageat treatment was 16.6 years old with 11 males and 18 females. 13 patients were referred for the low FODMAP diet. The patients referred for the low FODMAP diet were 7 with a functional gut disorder, 5Irritable Bowel Syndrome and1 EosinophilicColitis.The age range was 14 to 19 years old with

  17. Bases tratadas con cemento, en California

    Directory of Open Access Journals (Sweden)

    Chinchilla, M.

    1962-05-01

    Full Text Available El uso de bases tratadas con cemento para autopistas se inició en el Estado de California en 1938, empleándose para carreteras con determinadas condiciones de tráfico. Inicialmente, se especificó el uso obligatorio de plantas mezcladoras para asegurar el debido control de las proporciones adecuadas.

  18. LA LIBERTAD, EL SUEÑO PERENNE

    Directory of Open Access Journals (Sweden)

    Jesús Díaz

    2006-07-01

    Full Text Available In this essay, an interpretation of the concept of freedom is intended, based on the Herbert Marcuse principles. The Marcusian theories do not conceive the possibility of freedom existence in a capitalist system. It is necessary to transform the society to be pacified, and its production process to be automated in a way to liberate man physical force from the alienating work. Free time is the necessary conclusion of freedom. “Androgenization” of human activity will determine the conclusion of the relationship between man and woman. Woman will bring her kindness, solidarity, tenderness, among others; so to produce feminization of human society.

  19. LA LIBERTAD, EL SUEÑO PERENNE

    Directory of Open Access Journals (Sweden)

    Jesús Antonio Díaz Labarca

    2006-07-01

    Full Text Available En este ensayo, se pretende hacer una interpretación del concepto de libertad, según los principios de Herbert Marcuse. La teoría marcusiana no concibe la posibilidad de la existencia de la libertad en el sistema capitalista. Es necesario transformar la sociedad para que sea pacificada, y su proceso productivo sea automatizado de tal manera que se libere la fuerza física del hombre del trabajo alienado. El tiempo libre es la conclusión necesaria de la libertad. La “androgenización” de la actividad humana determinará la condición de la relación entre el hombre y la mujer. La mujer deberá aportar su bondad, solidaridad, ternura, etc. Para que se produzca la “feminización” de la sociedad humana.

  20. Fuerza manual de adultos con discapacidad intelectual

    Directory of Open Access Journals (Sweden)

    Ruth Cabeza Ruiz

    2017-09-01

    Full Text Available Objetivo. Presentar una descripción de la fuerza de prensión manual de hombres y mujeres con discapacidad intelectual (DI y comparar los resultados con valores de referencia de otras personas con y sin discapacidad intelectual. Método. El presente trabajo es un estudio transversal observacional, financiado por la Fundación SAMU, en el que se evaluaron a 122 personas con DI (86 hombres y 36 mujeres durante el desarrollo de unas jornadas de carácter recreativo en las que participaron varias asociaciones de atención a este colectivo. La batería de test utilizada fue el Alpaha-Fit Test Battery for Adults. Resultados. Se presentan los resultados relacionados con las variables de fuerza del miembro superior (Hand Grip Strength por grupos de edad (20-24, 25- 29, 30-34, 35-39, 40-44, 45-49, 50-54, 55-59 años. Los datos muestran valores que oscilan desde los 31 kg en los hombres más jóvenes con DI hasta los 13.3 kg del grupo más maduro de mujeres. Estos hallazgos son similares a los valores de referencia de población con DI española. Sin embargo, son muy inferiores a los obtenidos por la población sin discapacidad de la misma edad. Conclusión. Los resultados evidencian el menor rendimiento de las personas con DI en pruebas de fuerza de prensión manual por lo que se hace evidente la necesidad de llevar a cabo programas de ejercicio físico o deporte con las personas con DI.

  1. A study of the human immune response to Lolium perenne (rye) pollen and its components, Lol p I and Lol p II (Rye I and Rye II). II. Longitudinal variation of antibody levels in relation to symptomatology and pollen exposure and correction of seasonally elevated antibody levels to basal values.

    Science.gov (United States)

    Freidhoff, L R; Ehrlich-Kautzky, E; Meyers, D A; Marsh, D G

    1987-11-01

    This study used a standardized, dialyzed, Lolium perenne (ryegrass) pollen extract and two of its well-characterized components, Lol p I (Rye I) and Lol p II (Rye II), to characterize the longitudinal variation of both IgE and IgG antibody (Ab) levels, as well as total serum IgE levels, in 20 grass-allergic subjects followed for 13 months. Ab levels declined toward a basal level just before, and increased just after, the grass-pollination season, returning to the same basal level just before the next grass-pollination season. The least complex allergen, Lol II, demonstrated the most uniform pattern of variation in both IgE and IgG Ab levels. Total serum IgE levels demonstrated the least regular pattern of variation. Grass-pollen counts were strongly correlated with symptom-medication scores for these subjects (rs = 0.87). Initial values were correlated with the rise in total IgE and IgE Ab to Lol II across the grass-pollen season. Skin test results were correlated with initial IgE Ab levels for L. perenne pollen extract and Lol II. Finally, a procedure for correcting IgE Ab levels to basal values was proposed and tested. The correction procedure, for each IgE Ab, was based on the average rise during the grass-pollination season (or average decline after the grass-pollination season) observed for all subjects with that IgE Ab.

  2. Experiencias de haber crecido con un padre/madre con trastorno mental severo (TMS)

    OpenAIRE

    Vivanco B, Gabriela; Grandón F, Pamela

    2016-01-01

    Introducción. La experiencia de vivir con personas que presentan un Trastorno Mental Severo (TMS) es difícil para las familias, en especial para los hijos quienes han sido poco estudiados. El objetivo de la investigación fue conocer cómo la experiencia de haber vivido con un padre o madre con un trastorno mental severo influyó en la infancia, adolescencia y adultez joven de sus hijos e hijas. Método. Se analizan las experiencias de convivencia con un padre/madre con TMS en 10 hijos (6 hombres...

  3. El maltrato en las personas con discapacidad

    OpenAIRE

    Revuelta, Lucerga

    2014-01-01

    El maltrato no solo se realiza por acción sino también por omisión, la indiferencia hacia la persona con discapacidad es una forma de maltrato muy frecuente. Por ejemplo, ignorar y desatender las necesidades de la persona con discapacidad o, al contrario, la sobreprotección son maneras de maltrato. Cuando a un niño con discapacidad el padre o cuidador le hace todo, el niño se siente agredido pues le están incapacitando más de lo que su enfermedad ya lo hace.

  4. Hiperalgesia asociada al tratamiento con opioides

    OpenAIRE

    A. Gil Martín; M. Moreno García; J. Sánchez-Rubio Ferrández; T. Molina García

    2014-01-01

    La hiperalgesia inducida por opioides es una reacción paradójica caracterizada por una percepción intensificada de dolor relacionada con el uso de estos medicamentos en ausencia de progresión de la enfermedad o de síndrome de retirada. A diferencia de los casos de tolerancia, definida como pérdida de potencia analgésica durante el uso prolongado de opioides, no se produce mejoría con el escalado de dosis. La hiperalgesia inducida por opioides se ha manifestado en pacientes con dosis de manten...

  5. Tratamiento conservador en pacientes con retinoblastoma bilateral

    Directory of Open Access Journals (Sweden)

    Juan C. Suárez

    2008-11-01

    Full Text Available OBJETIVO: comparar el tratamiento convencional del retinoblastoma bilateral, usado hasta hace algunos años, consistente en radioterapia o enucleación bilateral, con el tratamiento conservador actual que incluye termoterapia transpupilar (TTT o TTT/quimioterapia al menos en un ojo, en niños con diagnóstico de retinoblastoma bilateral. DISEÑO: estudio retrospectivo descriptivo. MUESTRA: 20 pacientes con diagnóstico de retinoblastoma bilateral que consultaron al Hospital Universitario San Vicente de Paúl, de Medellín, Colombia, entre 1997 y 2007. MÉTODO: se hizo enucleación del ojo con el tumor de mayor tamaño. En el otro ojo se hizo tratamiento con TTT, con el láser diodo (810 nm, spot amplio, solo o combinado con otras terapias. RESULTADOS: se dividió a los pacientes en dos grupos: 16 pacientes (32 ojos en el grupo 1 tratados conservadoramente y 4 pacientes (8 ojos en el grupo 2 con tratamiento convencional. El rango de edad fue de 1-72 meses en el grupo 1 y de 1-12 meses en el grupo 2. El tiempo de seguimiento fue de 7-67 meses para el grupo 1 y de 13-73 meses para el grupo 2. En el grupo 1 se hizo enucleación de 16 ojos (50%, radioterapia externa de uno (3,1%, quimioterapia más termoterapia de 5 (15,6% y quimioterapia más termoterapia más crioterapia de 10 (31,3%. En todos los pacientes se logró preservar al menos un ojo. En el grupo 2, se enuclearon 7 ojos (87,5% y se hizo radioterapia externa más enucleación en un paciente (12.5%. Además, todos los pacientes recibieron quimioterapia. CONCLUSIÓN: la terapia conservadora actual consistente en tratamiento local (termoterapia, crioterapia o braquiterapia y quimiorreducción permite preservar al menos un ojo y en algunos casos de los dos, muchas veces con buena agudeza visual, en niños con retinoblastoma bilateral; se evitan así la enucleación bilateral y la radioterapia externa usada en el tratamiento convencional con todos sus efectos secundarios. La enucleación contin

  6. Paciente con tumor de cuerpo carotideo

    Directory of Open Access Journals (Sweden)

    Mariuska Forteza Sáez

    Full Text Available Los tumores de cuerpo carotideo (paragangliomas son neoplasias altamente vascularizadas, muy poco frecuentes y generalmente benignas, originadas en los quimiorreceptores del cuerpo carotideo. Se presenta el caso de un paciente de 54 años, con aumento de volumen cervical derecho, asintomático, con estudio preoperatorio y angiografía realizados por tomografía axial computarizada, que resultan compatibles con tumor de cuerpo carotideo. Se realiza disección subadventicial, informando la biopsia paraganglioma. El tumor fue completamente resecado, sin evidencia de recurrencia y sin complicaciones.

  7. EFECTO DE LA GERMINACIÓN SOBRE EL CONTENIDO Y DIGESTIBILIDAD DE PROTEÍNA EN SEMILLAS DE AMARANTO, QUINUA, SOYA Y GUANDUL EFEITO DA GERMINAÇÃO SOBRE O CONTEÚDO E DIGESTIBILIDADE DE PROTEÍNAS EM SEMENTES DE AMARANTO, QUINUA, SOJA E GUANDUL EFFECT OF THE GERMINATION ON THE PROTEIN CONTENT AND DIGESTIBILITY IN AMARANTH, QUINUA, SOY BEAN AND GUANDUL SEEDS

    Directory of Open Access Journals (Sweden)

    D.C. CHAPARRO ROJAS

    2010-06-01

    Full Text Available Se evaluaron los cambios en la concentración y digestibilidad de proteína durante la germinación en semillas de amaranto (Amaranthus sp, quinua (Chenopodium quinoa w., soya (Glycine max y guandul (Cajanus cajan. Las semillas utilizadas fueron suministradas por agricultores del Departamento del Cauca, seleccionadas asegurando calidad grado uno y porcentaje de germinación mayor al 90 %. Se estandarizó el método para la obtención de semillas germinadas, mediante la definición de variables como uso o no de desinfectante, tipo de sustrato, tiempo de germinación y temperatura. Se aplicó un diseño de bloques completos al azar con tres réplicas por día de germinación, para los días cero, uno, dos y tres; para cuantificación de proteína se utilizó Kjeldhal y para digestibilidad in-vitro de la proteína, se utilizó digestibilidad en pepsina. Los hallazgos permitieron concluir que la germinación induce cambios en la concentración y digestibilidad de la proteína de forma particular en cada tipo de semillas; en amaranto y soya, la germinación generó un incremento significativo en el contenido de proteína a partir del segundo día de germinación y en guandul a partir del primer día; en la quinua genero un descenso en el contenido de proteína en el segundo día, siendo estadísticamente igual en los días cero, uno y tres de germinación. La germinación mejoro la digestibilidad de la proteína, en semillas de quinua, guandul y soya, no generó cambios en semillas de amarantoSe avaliaram as mudanças na concentração e dlgistibiidade de proteína durante a germinação em sementes de amaranto (Amaranthus sp, quinua (Chenopodium quinoa w, soja (Glycine maxe guandul (Cajanus cajan. As sementes empregadas foram fornecidas por agricultores do Departamento do Cauca, selecionandoas para garantir a qualidade grau um e a porcentagem de germinação maior ao 90%. Padronizouse o método para a obtenção de sementes germinadas, mediante a

  8. Presa con revestimiento asfáltico

    Directory of Open Access Journals (Sweden)

    Proudfit, D. P.

    1960-07-01

    Full Text Available La presa denominada Montgomery se halla situada sobre el río South Platte River, a unos 8 km aguas arriba de la ciudad de Alma, del Estado de Colorado (EE. UU., y en la ladera oriental de la cordillera Continental Divide. El cuerpo o dique de esta presa está constituido por piedra, revestida con una capa de hormigón asfáltico en el paramento de contacto con el agua.

  9. Trastornos temporomandibulares en pacientes con maloclusiones

    Directory of Open Access Journals (Sweden)

    Luis Soto Cantero

    Full Text Available Introducción: existe una prevalencia de trastornos oclusales en gran medida relacionados con la presencia de disfunción temporomandibular. Objetivo: determinar el grado de disfunción temporomandibular según el Índice de Maglione en correspondencia con la prevalencia de maloclusiones en el área de salud del policlínico "Turcios Lima". Métodos: se realizó un estudio observacional descriptivo, de corte transversal, en la consulta de ortodoncia, de junio del 2008 a junio del 2009. De un universo constituido por los 280 pacientes que acudieron al servicio de ortodoncia en el período señalado con presencia de maloclusiones, se seleccionó una muestra de 84 pacientes por muestreo aleatorio simple, teniendo en cuenta los criterios de inclusión y exclusión realizados por criterios de expertos. Resultados: presentaban disfunción temporomandibular 74 pacientes y dentro de ellos 44 (52,4 % tenían disfunción grado II (Moderada. El 97 % de los pacientes con relación molar de clase II, presentaban disfunción temporomandibular. El 42,9 % de los pacientes con una maloclusión, presentaban disfunción grado I. El 60 % de los pacientes con dos maloclusiones presentaban disfunción grado II y el 66,7 % de los pacientes con tres maloclusiones, presentaban disfunción grado III. La mayor cantidad de pacientes tuvieron disfunción grado II (Moderada. Conclusiones: el mayor por ciento de los pacientes con disfunciones presentó una clase II molar y a medida que aumentó el número de maloclusiones aumentó también la severidad de la disfunción.

  10. RadCon: A Radiological Consequences Model

    International Nuclear Information System (INIS)

    Crawford, J.; Domel, R.U.

    2000-05-01

    RadCon estimates the dose received by user selected groups in the population from an accidental release of radionuclides to the environment. The exposure pathways considered are external exposure from the cloud and ground and internal exposure from inhalation and ingestion of contaminated food. Atmospheric dispersion modelling is carried out externally to RadCon.Given a two dimensional time varying air and ground concentration of radioactive elements, RadCon allows the user to: view the air and ground concentration over the affected area, select optional parameters and calculate the dose to people,display the results to the user, and change the parameter values. RadCon offers two user interfaces: 1) the standard graphical user interface which is started using Java DoseApp at the command line, or by setting up a shortcut to this command (particularly when RadCon is installed on a PC) and 2) the text based interface used to generate information for the model inter-comparison exercise . This is initiated using Java BIOMASS at the command line, or an equivalent shortcut. The text based interface was developed for research purposes and is not generally available. Appendices A, B and C provide a summary of instructions on setting up RadCon. This will generally be carried out by the computer support personnel

  11. Adolescente femenino con granulomatosis de Wegener fulminante

    Directory of Open Access Journals (Sweden)

    S C Scheffler-Mendoza

    2017-01-01

    Full Text Available Adolescente femenino de 13 años de edad, originaria de Guerrero, grupo étnico mixteco, padres analfabetos y con poco entendimiento del español. Sin otros antecedentes de importancia para el padecimiento. Inició un mes previo con la aparición de una pápula hipercrómica en párpado superior derecho que en los 10 días previos al ingreso aumentó de volumen generando proptosis. Al interrogatorio se negó dolor, lagrimeo, fiebre, o pérdida de peso. Ingresó con fiebre (39.0°C, palidez generalizada, proptosis derecha con exposición de córnea y conjuntiva, eritema periorbitario, movimientos oculares y agudeza visual disminuidos (i.e. contaba dedos a 1 m, pupila con tendencia a midriasis, hiperreactividad a la luz, y fondo de ojo con papila hiperémica edematosa y tortuosidad peripapilar. El resto de exploración no mostró datos relevantes. Se hospitalizó para iniciar tratamiento antimicrobiano parenteral de amplio espectro (i.e. ceftriaxona y vancomicina y abordar proptosis unilateral.

  12. Alimentación de bovinos con ensilado de mezclas de banano de rechazo y ráquis en diferentes proporciones

    Directory of Open Access Journals (Sweden)

    J. C. De la Cruz-Hernández

    2006-01-01

    Full Text Available Con el propósito de evaluar el comportamiento productivo de bovinos consumiendo ensilado de mezclas de banano de rechazo como fruta y ráquis en diferentes proporciones, se realizó una prueba de alimentación con 12 bovinos cebú comercial, machos, enteros, con un peso promedio de 168±17 kg, a los que se les asignó, de manera aleatoria, cada uno de los siguientes tratamientos: I ensilado con 50% banano y 50% ráquis; II ensilado con 75% banano y 25% ráquis; y III un grupo testigo con zacate Taiwán (Pennisetum purpureum. Los animales recibieron, además, 1.1 kg de pasta de soya como fuente proteica y 50 g de una premezcla comercial de minerales y sal común. La prueba tuvo una duración de 120 días, más un tiempo de adaptación de 15, periodo en el que las dietas se ofrecieron a libertad. Los animales fueron pesados cada 30 días y se les llevó diariamente el control de consumo de alimento. Las mejores (P0.05 entre ellos. El consumo de alimento base húmeda (CABH no presentó diferencias significativas (P>0.05 entre tratamientos; sin embargo, el consumo de alimento base seca (CABS fue mayor (P<0.05 en el tratamiento ensilado de banano-ráquis 75:25 comparado con el de 50:50 (6.433 vs. 4.308 kg. El consumo de materia seca/100 kg de peso vivo fue mayor (P<0.05 para los animales que consumieron zacate Taiwán y ensilado de banano-ráquis 75:25 (2.334 y 1.990, comparados con los del ensilado 50:50 (1.413. El consumo de proteína cruda en kg fue mayor (P<0.05 para los animales alimentados con zacate Taiwán. Porcentualmente, el consumo de proteína cruda fue mayor (P<0.05 en los animales que consumieron zacate Taiwán y ensilado de banano-ráquis 50:50 (16.99 y 17.36 %. La mayor eficiencia alimenticia (P<0.05 la obtuvieron los animales que consumieron el ensilado de banano-ráquis 50:50. El análisis económico de este sistema de alimentación nos indica que se obtuvieron las mayores ganancias con el uso de los ensilados, siendo el mejor

  13. RadConEd: A Graphical Data Editor for the Radiological Consequences Model, RadCon

    International Nuclear Information System (INIS)

    Crawford, J.; Domel, R.U.

    2000-05-01

    This document describes the application, RadConEd, which has been designed and implemented to enable users of the RadCon system to update these parameter files. The RadCon system is written in the Java programming language, and as such provides portability across computer platforms. The software described in this report was developed in line with the portability requirements of RadCon, thus providing a uniform user interface across computer platforms and bypassing the need of using system editors. In addition a number of data integrity measures were implemented

  14. Efectos de la suplementación con bloques multinutricionales a base de nopal fermentado sobre la ganancia de peso de ovinos en crecimiento Efectos de la suplementación con bloques multinutricionales a base de nopal fermentado sobre la ganancia de peso de ovinos en crecimiento

    Directory of Open Access Journals (Sweden)

    Isidro Guajardo Hernández

    2012-02-01

    Full Text Available Este trabajo presenta una alternativa práctica del aprovechamiento del nopal mediante la elaboración de Bloques Multinutricionales (BM y su utilización en la alimentación de corderos en crecimiento. Al nopal fresco picado se le adicionó sorgo, soya, urea, minerales y yogurt natural, se dejó fermentar por dos semanas a temperatura ambiente y se elaboraron los BM con un 25 % de nopal fermentado. Se utilizaron 20 corderos destetados con un peso vivo promedio de 17 Kg distribuidos en dos grupos (con y sin BM, ambos grupos recibieron una dieta típica. Durante 57 días de experimentación se midió el consumo de alimento, consumo de BM y el peso de los corderos. Los pesos de los animales fueron analizados estadísticamente mediante un análisis de varianza con un diseño completamente al azar. No se encontraron diferencias estadísticamente significativas (P > .05 en los pesos de los animales. El BM elaborado con nopal fermentado fue consumido adecuadamente por los animales.This paper presents a practical alternative use for the prickly pear cactus as a principalcomponent in the elaboration of multi-nutritional blocks (BMs used to feed growing sheep.Sorghum, soy bean, urea minerals, and natural yogurt were added to the cactus, the mixturewas fermented for two weeks at room temperature, and then the BMs were made consisting25 % of the fermented mixture. Twenty weaned sheep with an average weight of 17 kg wererandomly assigned to one of two groups (with or without BMs, and both were fed a typicaldiet for growing sheep. Feed intake, BM intake, and body weight were registered overa 57 day period. Body weight was analyzed statistically through ANOVA in a completelyrandomized design. No statistically significant differences were observed in body weight.BMs made with fermented prickly pear cactus were adequately consumed by the sheep.

  15. Enfermedad coronaria en pacientes con psoriasis

    Directory of Open Access Journals (Sweden)

    Walter Masson

    2013-10-01

    Full Text Available Comunicaciones previas asociaron la psoriasis con la enfermedad coronaria. Desconocemos si en nuestro país o región existe dicha asociación. Se realizó un estudio transversal analizando los datos de la historia clínica electrónica de un sistema de salud de Buenos Aires. Analizamos todos los pacientes mayores de 18 años con diagnóstico de psoriasis entre el 1 de enero de 2003 y el 31 de julio de 2011 y los comparamos con un grupo control, en una relación 2:1, obtenido en forma aleatoria del mismo sistema de salud, apareados por edad y sexo. Determinamos la prevalencia de los factores de riesgo cardiovascular y de enfermedad coronaria. Analizamos la asociación entre la enfermedad coronaria y la psoriasis mediante análisis uni y multivariado. Se incluyeron 3 833 sujetos (1 286 pacientes con psoriasis y 2 547 controles. La prevalencia de hipertensión arterial (50% vs. 38%, p < 0.001, tabaquismo (25% vs. 17%, p < 0.001, diabetes (12% vs. 8%, p < 0.001 y enfermedad coronaria (4.98% vs. 3.06%, p = 0.003 fue mayor en los sujetos con psoriasis en comparación con el grupo control. Independientemente de la edad, la presencia de diabetes, hipertensión arterial o tabaquismo, hubo una asociación significativa entre la enfermedad coronaria y la psoriasis (OR 1.48, IC95% 1.04-2.11, p = 0.03. En conclusión, en esta población de Buenos Aires, los pacientes con psoriasis tuvieron una mayor prevalencia de diabetes, hipertensión arterial, tabaquismo y enfermedad coronaria. La asociación entre la psoriasis y la enfermedad coronaria fue independiente de los factores de riesgo explorados.

  16. Mincle Signaling Promotes Con-A Hepatitis

    Science.gov (United States)

    Greco, Stephanie H.; Torres-Hernandez, Alejandro; Kalabin, Aleksandr; Whiteman, Clint; Rokosh, Rae; Ravirala, Sushma; Ochi, Atsuo; Gutierrez, Johana; Salyana, Muhammad Atif; Mani, Vishnu R.; Nagaraj, Savitha V.; Deutsch, Michael; Seifert, Lena; Daley, Donnele; Barilla, Rocky; Hundeyin, Mautin; Nikifrov, Yuriy; Tejada, Karla; Gelb, Bruce E.; Katz, Steven C.; Miller, George

    2016-01-01

    Concanavalin-A (Con-A) hepatitis is regarded as a T cell-mediated model of acute liver injury. Mincle is a C-type lectin receptor (CLR) that is critical in the immune response to mycobacteria and fungi, but does not have a well-defined role in pre-clinical models of non-pathogen mediated inflammation. Since Mincle can ligate the cell death ligand SAP130, we postulated that Mincle signaling drives intrahepatic inflammation and liver injury in Con-A hepatitis. Acute liver injury was assessed in the murine Con-A hepatitis model using C57BL/6, Mincle−/−, and Dectin-1−/− mice. The role of C/EBPβ and HIF-1α signaling was assessed using selective inhibitors. We found that Mincle was highly expressed in hepatic innate inflammatory cells and endothelial cells in both mice and humans. Furthermore, sterile Mincle ligands and Mincle signaling intermediates were increased in the murine liver in Con-A hepatitis. Most significantly, Mincle deletion or blockade protected against Con-A hepatitis whereas Mincle ligation exacerbated disease. Bone marrow chimeric and adoptive transfer experiments suggested that Mincle signaling in infiltrating myeloid cells dictates disease phenotype. Conversely, signaling via other CLRs did not alter disease course. Mechanistically, we found that Mincle blockade decreased the NF-κβ related signaling intermediates, C/EBPβ and HIF-1α, both of which are necessary in macrophage-mediated inflammatory responses. Accordingly, Mincle deletion lowered production of nitrites in Con-A hepatitis and inhibition of both C/EBPβ and HIF1-α reduced the severity of liver disease. Our work implicates a novel innate immune driver of Con-A hepatitis and, more broadly, suggests a potential role for Mincle in diseases governed by sterile inflammation. PMID:27559045

  17. Mincle Signaling Promotes Con A Hepatitis.

    Science.gov (United States)

    Greco, Stephanie H; Torres-Hernandez, Alejandro; Kalabin, Aleksandr; Whiteman, Clint; Rokosh, Rae; Ravirala, Sushma; Ochi, Atsuo; Gutierrez, Johana; Salyana, Muhammad Atif; Mani, Vishnu R; Nagaraj, Savitha V; Deutsch, Michael; Seifert, Lena; Daley, Donnele; Barilla, Rocky; Hundeyin, Mautin; Nikifrov, Yuriy; Tejada, Karla; Gelb, Bruce E; Katz, Steven C; Miller, George

    2016-10-01

    Con A hepatitis is regarded as a T cell-mediated model of acute liver injury. Mincle is a C-type lectin receptor that is critical in the immune response to mycobacteria and fungi but does not have a well-defined role in preclinical models of non-pathogen-mediated inflammation. Because Mincle can ligate the cell death ligand SAP130, we postulated that Mincle signaling drives intrahepatic inflammation and liver injury in Con A hepatitis. Acute liver injury was assessed in the murine Con A hepatitis model using C57BL/6, Mincle(-/-), and Dectin-1(-/-) mice. The role of C/EBPβ and hypoxia-inducible factor-1α (HIF-1α) signaling was assessed using selective inhibitors. We found that Mincle was highly expressed in hepatic innate inflammatory cells and endothelial cells in both mice and humans. Furthermore, sterile Mincle ligands and Mincle signaling intermediates were increased in the murine liver in Con A hepatitis. Most significantly, Mincle deletion or blockade protected against Con A hepatitis, whereas Mincle ligation exacerbated disease. Bone marrow chimeric and adoptive transfer experiments suggested that Mincle signaling in infiltrating myeloid cells dictates disease phenotype. Conversely, signaling via other C-type lectin receptors did not alter disease course. Mechanistically, we found that Mincle blockade decreased the NF-κβ-related signaling intermediates C/EBPβ and HIF-1α, both of which are necessary in macrophage-mediated inflammatory responses. Accordingly, Mincle deletion lowered production of nitrites in Con A hepatitis and inhibition of both C/EBPβ and HIF-1α reduced the severity of liver disease. Our work implicates a novel innate immune driver of Con A hepatitis and, more broadly, suggests a potential role for Mincle in diseases governed by sterile inflammation. Copyright © 2016 by The American Association of Immunologists, Inc.

  18. Evaluación de la soya integral cocida y de harina de yuca en la alimentación de aves de postura

    Directory of Open Access Journals (Sweden)

    Santos V. Eduardo

    1987-03-01

    Full Text Available Se localizaron poblaciones de la planta en los municipios de Santa Rosa-Cauca y de Mocoa-Putumayo y se hicieron observaciones fenológicas discontinuas entre abril de 1984 y agosto de 1986; simultáneamente se realizaron ensayos de propagación sexual y asexual (estacas y acodos. La floración, fructificación y emisión de follaje se presentaron con cierta periodicidad durante el período de observaciones, en tanto que la caída de follaje no ocurrió en volúmenes apreciables. Los ensayos de propagación sexual fracasaron, mientras que el enraizamiento de estacas, aún utilizando enraizadores, fue muy bajo (6.5 %; las únicas estacas que enraizaron fueron las terminales sembradas con yema foliar y hojas apicales. Todos los acodos formaron callo pero enraizaron más los tratados con enraizadores (75 %.This paper presents phenological, sexual and asexual propagation observations of Elaeagia pastoensis Mora, furthermore some botanic and ecology aspects of the plant were conssidered. The plant was found growing in Santa Rosa-Cauca and Mocoa-Putumayo municipalities where discontinual phenological observations were made; sexual and asexual (stem cutting and aereal layer propagation experiments were made too. The field work carried out through april 1984 and august1986. Flowering, fruiting and leaf flushing appeared with some regularity during the observations period; the leaf fall was not observed in siqnificant quantyties. The sexual propagations was not sucesfully. The percentage of root setting taken from terminal of the branch with resinal bud leaves and apical leaves set roots. AII aerel layer formed callus, however the hi- ghest percentage of root setting (75% was reached with treated ones with hormones.

  19. Elaboración de barras energéticas para escolares a partir de subproductos industriales de soya y maíz

    OpenAIRE

    De La Paz Castro, Viviana; Coello Ojeda, Karín Elizabeth

    2013-01-01

    El Programa de Alimentación Escolar del Ecuador (PAE) ofrece a escuelas fiscales productos como complemento alimenticio que contienen un adecuado aporte de calorías, proteínas, vitaminas y minerales con el propósito de mejorar la nutrición y por consiguiente la capacidad en el aprendizaje de los niños en edad escolar. Entre los productos que el PAE distribuye se encuentra la granola en barra hecha a base de avena, la cual al ser una materia prima importada eleva el costo del producto final. ...

  20. Kazein, Kazeinat ve Soya Proteini ile Hazırlanan Taklit Taze Kaşar Peynirlerinin Fizikokimyasal ve Tekstürel Özellikleri (İngilizce

    Directory of Open Access Journals (Sweden)

    Pınar Balkır

    2015-02-01

    Full Text Available Bu çalışmada, dört farklı tipte hileli ve iki tipte taklit taze kaşar peyniri üretilmiştir. Hileli taze kaşar üre­timlerinde elastik telemeye, her birinin erimiş kitledeki oranı %5 olacak şekilde rennet kazein, sodium kazeinat, kalsiyum kazeinat ve soya proteini izolatı katılmıştır (sırasıyla CRC, CSC, CCC, CSOY. Birinci parti taklit peynirlerin (R1 üretiminde rennet kazein, soya proteini izolatı, hidrojene pamuk yağı, NaCl, carrageenan, emülgatör, ikinci parti taklit peynirlerin (R2 üretiminde ise NaCl, modifiye nişasta, hidrojene pamuk yağı, su ve emülgatör kullanılmıştır. Kontrol amacıyla yerel bir süt işletmesinden taze kaşar pey­nirleri temin edilmiştir. Tüm peynir örneklerinde fizikokimyasal analizler, tekstür profil analizleri ve erime analizleri yapılmıştır. Taklit taze kaşar gruplarının kontrol ile karşılaştırıldığında, kurumadde (P<0.01 ve protein (P<0.01 içeriğinin daha düşük, kurumadde yağ içeriğinin ise daha yüksek olduğu görülmüştür (P<0.01. Tekstürel özellikler ve eriyebilirlik açısından en iyi sonuçlar rennet kazein içeren grup ve R2 grup­larında elde edilmiştir.

  1. Variables socionutricionales de hogares mazahuas integrados por preescolares desnutridos con madres con obesidad y sin obesidad

    Directory of Open Access Journals (Sweden)

    Conzuelo González, Viridiana Vanessa

    2009-01-01

    Full Text Available El primer objetivo fue conocer cuántos menores de cinco años con diferentes grados de desnutrición tienen una madre con sobrepeso/obesidad/ en una comunidad indígena que vive en extrema pobreza y bajo condiciones de migración masculina internacional. El segundo fue comparar tres variables socionutricionales (ingreso familiar, educación de la madre y adecuación nutrimental de la dieta diaria entre estos hogares y los hogares con desnutrición infantil y madres sin obesidad. Se realizó un estudio transversal (2006-2007, en la comunidad mazahua de San Francisco Tepeolulco, Municipio de Temascalcingo; que incluyó a 85 hogares integrados por preescolares con desnutrición inscritos al programa Oportunidades. Se determinó el estado nutrición de los preescolares con indicadores antropométricos y se obtuvo el IMC de las madres de estos infantes. Se aplicó una encuesta socionutricional, incluida el recordatorio de 24 horas, y complementado con la observación participante (cualitativa. Se encontró que 83% de las madres mazahuas presentaron sobrepeso u obesidad. El estado de nutrición de los preescolares con madres con obesidad presentó un porcentaje mayor de desnutrición (76%. En la variable género, se encontró que 54% de los niños con madres con obesidad tenía baja talla. Al relacionar el nivel educativo de la madre, esta variable resultó ser estadísticamente significativa (p=0.015, donde el analfabetismo está más relacionado con la desnutrición infantil que tienen madres de bajo y/o peso normal. La elevada prevalencia de hogares conformados con preescolares con desnutrición y madres con obesidad, es un síntoma más de la pobreza en zonas indígenas en México, con bajo índice de desarrollo humano.

  2. Epilepsia de la infancia con paroxismos occipitales

    Directory of Open Access Journals (Sweden)

    Albia Pozo Alonso

    1999-09-01

    Full Text Available Se reporta que la epilepsia de la infancia con paroxismos occipitales constituye un síndrome epiléptico muy bien definido; se incluye en el grupo de las epilepsias idiopáticas relacionadas con localización. Los ataques comienzan habitualmente con síntomas visuales, en ocasiones seguidos por ataques hemiclónicos, parciales complejos y tónicoclónicos generalizados. El electroencefalograma interictal muestra complejos de puntaonda de localización occipital y temporal posterior que desaparecen con la apertura de los ojos. Se presenta una paciente de 8 años de edad que reúne los criterios clínicos y electroencefalográficos de la epilepsia de la infancia con paroxismos occipitales y se enfatiza en la evolución supuestamente benigna de dicho síndrome.Is is reported that epilepsy of childhood with occipital paroxysms is an epileptic syndrome that is well defined. It is included in the group of related idiopathic epilepsies with localization. Seizures habitually begin with visual symptoms, which are ocassionally followed by hemiclonic, complex partial, and generalized tonicclonic seizures. The interictal EEG shows spike-wave complexes of occipital and temporal posterior localization that are suppressed by eye opening. A patient aged 8 in whom the clinical and electroencephalographic criteria of epilepsy of childhood with occipital paroxysms are present is studied in this paper. Emphasis is made on the apparently benign evolution of this syndrome.

  3. Aspectos Psicosociales Relacionados con el TEPT en Pacientes con Cáncer de Mama

    OpenAIRE

    Hernández Moreno, Fresia Paloma; Landero Hernández, René

    2015-01-01

    El objetivo del estudio fue analizar la relación del estrés, la depresión y las estrategias de afrontamiento con el Trastorno de Estrés Postraumático (TEPT) en una muestra de pacientes con cáncer de mama. Se realizó con una muestra no probabilística de 52 mujeres con cáncer de mama. Se encontró una relación entre TEPT y estrés (r= .344, p= .014), TEPT y depresión (rs=.346, p= .013). El TEPT correlacionó con evitación cognitiva (r= .437, p= .001), TEPT y desesperanza (rs= .437, p= .001) y TEPT...

  4. Desarrollo de aleaciones multifuncionales con base titanio

    OpenAIRE

    Pareja, Ramiro

    2007-01-01

    El Laboratorio de Materiales Nano-estructurados y Multifuncionales del Departamento de Física de la Universidad Carlos III de Madrid se dedica al desarrollo, procesado y caracterización de aleaciones con propiedades mejores que las preparadas por métodos convencionales. La mejora de las propiedades se consigue produciendo las aleaciones con un tamaño de grano nanométrico o ultrafino, o introduciendo una dispersión de nano-partículas de óxido en la matriz. El grupo está interesado en algún tip...

  5. TIROTOXICOSIS GESTACIONAL: PATOLOGIA CON RIESGO VITAL

    OpenAIRE

    Valdés R.,Enrique; Pilasi M.,Carlos; Núñez U.,Tatiana

    2003-01-01

    Se presenta un caso clínico con diagnóstico final de Tirotoxicosis gestacional que debuta con una complicación excepcional, insuficiencia cardíaca congestiva e hipertensión pulmonar severa. Se presenta la experiencia del Hospital Clínico de la Universidad de Chile, proponiendo que su diagnóstico y tratamiento oportunos son la base del pronóstico de esta patología de riesgo vital para el binomio madre-hijo

  6. Amplificadores con transistores. Estudio y dimensionado

    OpenAIRE

    Lubiano García, Adrián

    2017-01-01

    Este trabajo es un estudio de las distintas configuraciones de los amplificadores con transistores vistos en la asignatura de Electrónica Analógica del tercer curso del Grado en Ingeniería en Electrónica Industrial y Automática de la Escuela de Ingenierías Industriales de la Universidad de Valladolid. En este trabajo se mostrarán los pasos seguidos en la creación de una aplicación con Visual Basic para la realización de los ejercicios de las distintas configuraciones, así...

  7. Galaxias australes con núcleo doble

    Science.gov (United States)

    Gimeno, G.; Díaz, R.; Carranza, G.

    Se estudia una muestra de galaxias australes con núcleo doble a partir de una búsqueda extensiva en la literatura. Se analizan las características morfológicas, fotométricas y espectroscópicas de la muestra. Para algunas galaxias se han realizado observaciones con el espectrógrafo multifunción (EMF) de la Estación Astrofísica de Bosque Alegre a partir de las cuales se determinaron parámetros cinemáticos.

  8. Efecto del boro, zinc y el azufre sobre la doble finalidad (forraje y grano del cultivo de soya Glycine max (L Merr

    Directory of Open Access Journals (Sweden)

    Restrepo S. L. Diego

    1987-12-01

    Full Text Available El diseño experimental empleado fue el de bloques completamente al azar, con cinco repeticiones y nueve tratamientos constituidos por: un testigo absoluto (no podado, ni fertilizado, un testigo normal (podado, no fertilizado, el resto de tratamientos fueron podados y fertilizados de la siguiente manera: boro, zinc, azufre, boro + zinc, boro + azufre, zinc + azufre, boro + zinc + azufre, esta fertilización se hizo al suelo al momento de la siembra. La variedad empleada fue Andree-23 con una densidad de población de 600.000 plantas /ha, con surcos separados a 40 cm, el corte se realizó a los 38 días de emergencia o sea al inicio de floración a un 40% de altura. La poda total ejerce efecto negativo sobre la producción de grano al reducirlo en un 53%, pero aumenta el número de ramas. La fertilización solo fue favorable en la altura de planta y en la reducción de vainas sin semilla; en las demás variables (número pe vainas/planta, número de semillas/ vaina, peso promedio de cien semillas, etc. no se presentó ninguna incidencia.The experiment pretended to watch the soybean's behavior submited to pruness by realizing fertilization with boron, cinc and sulphur and their influence over seed and f or age yield. Experimental design used was blocks by random completely, with five repetitions and nine treatments set up by: an absolute control (no pruned, no fertilized, a normal control (pruned, no fertilized, other treatments were pruned and fertilized by this way: boron, cinc, sulphur, boron + cinc, boron + sulphur, cinc + sulphur, boron + cinc + sulphur, this fertilization was made to soil in sowing moment. The variety was Andree-23 (600.000 plants /ha., with forrows separated by 40 cm, cutting was made 38 days after emergetion. The whole pruness makes a negative effect over seed yield by decreasing it about 53 percent, but arise branche number. Fertilization only was good for height plant and empty pods reduction; over other variables (pod

  9. IoT con Raspberry Pi

    OpenAIRE

    García Muelas, Carlos

    2015-01-01

    Estudio de diferentes plataformas software y hardware del internet de las cosas. Aplicación real con Raspberry Pi. Estudi de diferents plataformes programari i maquinari de l'internet de les coses. Aplicació real amb Raspberry Pi. Bachelor thesis for the Telecommunication Technologies program on Telematics.

  10. en personas con el VIH/SIDA

    Directory of Open Access Journals (Sweden)

    Ximena Palacios Espinosa

    2006-01-01

    Full Text Available El objetivo de este estudio fue hallar la relación de la intención e ideación suicida con algunas variables sociodemográficas, las características de la enfermedad, el tratamiento antirretroviral y los aspectos piscoafectivos en personas con el VIH/SIDA que residen en Bogotá (Colombia y pertenecientes a una fundación. Para ello, se tomó una muestra de 75 pacientes con VIH positivo a quienes se evaluó a través de una entrevista semiestructurada y dos instrumentos: el inventario de depresión de Beck (IBD y la escala de Ideación Suicida (SSI. Los resultados mostraron que solo 29 personas tenían ideación suicida y 10 intención suicida: De los 10 solo 2 sujetos tenían intención después del diagnóstico. Se encontró una relación estadística significativa para ideación suicida e intención suicida con edad, estrato socioeconómico, estado civil, orientación sexual, las características de la enfermedad, el tratamiento antirretroviral y los aspectos piscoafectivos.

  11. La relazione empatica con i robot

    NARCIS (Netherlands)

    Marti, P.; Iacono, I.; Tittarelli, M.

    2013-01-01

    È possibile instaurare una relazione empatica tra un essere umano e un robot? Il presente studio valuta le soluzioni di design adottate nella progettazione di una maschera empatica, implementata su un tablet, che consente all’anziano di dialogare nell’ambiente domestico con un robot che lo assiste

  12. Sarcoma de Kaposi en paciente con SIDA

    Directory of Open Access Journals (Sweden)

    Jesús Ramón León Polanco

    2015-01-01

    Full Text Available Se presenta el caso de un paciente masculino de 33 años de edad, con antecedentes de VIH-SIDA desde hace 10 años, que se mantiene en tratamiento con antirretrovirales. Durante todo este tiempo ha presentado varios episodios de infecciones respiratorias, incluyendo tuberculosis pulmonar 5 años atrás. Acude a consulta refiriendo edemas en miembros inferiores acompañado de lesiones en piel de color violáceo de un año de evolución, previamente interpretado como linfangitis rebelde al tratamiento y que se extendió a la cara interna de los muslos y a los miembros inferiores. Con pérdida de peso, no prurito en las lesiones, fiebre, lesiones en la mucosa oral. Se determinó hemoglobina 89 g/L, leucocitos 4,5 x 109 /L, se estudiaron las funciones hepática y renales resultando normales. Radiografías de tórax y ultrasonido abdominal normales. Se realizó estudio anatomopatológico de piel que informó Sarcoma de Kaposi. Se impuso tratamiento con quimioterapia

  13. Las construcciones con esqueleto en madera

    Directory of Open Access Journals (Sweden)

    Heinz Leser S.

    1984-06-01

    Estos sistemas se emplean preferentemente en viviendas prefabricadas y edificios de pequeña envergadura. Finalmente, consideramos las construcciones con "esqueleto en madera", cuyo campo de aplicación principal está en las edificaciones de mediana extensión: escuelas, centros comunitarios, pequeñas industrias o bloques habitacionales.

  14. Conversazioni autobiografiche con Albino Sacco-Casamassima

    Directory of Open Access Journals (Sweden)

    Orazio Maria Valastro

    2004-03-01

    Full Text Available Nel 1948 venne Olivetti a Matera. Olivetti venne a Matera con un certo ambasciatore americano che praticamente aveva preparato o stava dando una mano per il piano Marschall, venne a Matera e mi vollero incontrare. Io sono stato chiamato dalla piazza, stavo nella piazza, era il mese di luglio, una cosa di questo genere, e andai in un bar, un bar ad angolo mi ricordo in Via Don Minzioni, dove questo Olivetti mi disse 'ma lei che cosa fa coi sassi?' 'Che cos'è questa storia?' Gli raccontai un po' che cosa facevo io con questi amici, avevamo fatto questo, insomma tutto quello che avevamo fatto nei sassi e che volevamo preparare ancora per presentare a qualcuno un piano di revisionamento dei sassi. Mi ricordo, era la prima volta che avevo visto Adriano Olivetti, ne avevo sentito parlare però non avevo mai visto quest'uomo con gli occhi celesti, minuto, coi capelli bianchi, riccioluto, con una cravatta bianca, cosa che mi fece impressione, una specie di angelo.

  15. Procesamiento de argumentos en personas con afasia

    Directory of Open Access Journals (Sweden)

    Luis Martínez

    2012-06-01

    Full Text Available En las personas con afasia se alteran diversos procesos lingüísticos. Entre las alteraciones más comunes tenemos aquellas que se producen en el procesamiento sintáctico, lo cual es evidente en el procesamiento de los argumentos. Investigaciones previas realizadas a sujetos con afasia reflejan un procesamientodeficiente de argumentos, lo cual se hace más evidente a medida que el número de argumentos aumenta. Se ha encontrado que mientras mayor es la complejidad en la estructura de la oración, los sujetos afásicos manifiestan mayores dificultades en el procesamiento de la misma. El presente estudio indaga en las dificultades que presentan los sujetos adultos con trastornos afásicos hablantes de español en el procesamiento de argumentos durante tareas de descripción. Este trabajo corrobora la evidencia de que los sujetos con afasia manifiestan un manejo deficiente en el procesamiento de argumentos en las tareas señaladas.

  16. Coriocarcinoma con metástasis pulmonar

    Directory of Open Access Journals (Sweden)

    Vicia Sánchez Abalos

    2014-05-01

    Full Text Available Se presenta el caso clínico de una fémina de 44 años de edad, con 32 semanas de embarazo, la cual fuera ingresada en la Unidad de Cuidados Intensivos del Hospital General Docente "Dr. Juan Bruno Zayas Alfonso" de Santiago de Cuba, por presentar insuficiencia respiratoria aguda como consecuencia de una sepsis. La paciente fue tratada con cefalosporina de tercera generación y ventilación mecánica no invasiva, pero se mantuvieron las características gasométricas de hipoxemia y una mala reacción terapéutica, por lo que se requirió instrumentación de las vías respiratorias y soporte hemodinámico, sin lograr regresión del cuadro clínico, lo cual condujo a un paro cardiorrespiratorio y, con ello, a la muerte. La necropsia mostró un coriocarcinoma del endometrio con metástasis pulmonar

  17. Con la testa fra le nuvole

    Directory of Open Access Journals (Sweden)

    André Greboge

    2015-06-01

    Full Text Available Partitura de Con la testa fra le nuvole, composta em 2015 para piano e harpa de pedais. Este memorial apresenta as notas sobre a obra e a organização do processo composicional, baseada em um insight composicional a partir da ideia de complexidade apresentada por James Tenney em Meta Meta-hodos (1988.

  18. REDUCCIÓN DE ÓXIDOSDE NITROGENO CON CATALIZADORES ZEOLÍTICOS INTERCAMBIADOS CON COBALTO

    Directory of Open Access Journals (Sweden)

    Enrique Alexis García Moya

    2012-10-01

    Full Text Available Se presenta un estudio sobre la reducción catalítica selectiva (RCS de NOx con metano en presencia de oxígeno en exceso con varios catalizadores a base de las zeolitas mordenita, ferrierita y ZSM-5 intercambiadas con diferentes cargas de cobalto y con las correspondientes zeolitas en forma acida. Cuando la mezcla reactiva contenía mayormente NO2 en lugar de NO, los catalizadores ácidos mostraron las más altas velocidades de formación de N2 en condiciones secas. Las mayores actividades se obtuvieron con los catalizadores Co-mordenita, siguiendo en orden de actividad los catalizadores Co-Ferrierita y Co-ZSM-5. El catalizador Co-Mordenita más activo se ensayó con una mezcla reactiva donde predominó el NO en lugar del NO2 bajo condiciones secas e hidrotérmicas y en presencia de SO2. Con la adición de 8% de agua a la mezcla de reacción se observó desactivación reversible, especialmente a bajas temperaturas. La adición de 60 ppm de SO2 disminuyó la velocidad de reacción aproximadamente a la mitad debido posiblemente al envenenamiento de algunos sitios activos.

  19. Manejo de conductos radiculares con curvatura marcada

    Directory of Open Access Journals (Sweden)

    Jorge Mario Nuñez Duran

    2013-10-01

    Full Text Available Las variaciones anatómicas en dientes con conductos que poseen dilaceraciones hacen del tratamiento de endodonciaun reto o una complicación relativa, en donde el clínico debe desarrollar toda su habilidad y el uso de instrumentosespeciales que faciliten u optimicen la terapia de conductos radiculares. La toma de radiografías previas con diferentesangulaciones y el estudio minucioso de ellas, darán una ayuda fundamental para conocer más detalladamente estacomplicación anatómica, que en algunos casos es muy frecuente. El uso de instrumental más flexible y resistentepermite llegar con mayor precisión y seguridad a la parte apical sin que se corra el riesgo de fracturar los instrumentos,limpiándolos y modelándolos en toda su extensión para poder obturarlos de una manera correcta.El uso de limas de menor calibre, precurvadas, permite acceder a la porción dilacerada de la raíz. Este es un métodoeficaz para prevenir la perdida de longitud de trabajo y evitar así las perforaciones de las raíces durante la preparación.Dentro de los errores más comunes durante la instrumentación de los conductos está la perdida de longitud detrabajo, junto con la transportación del foramen apical, ya sea de manera externa o interna. En el presente artículose menciona el uso de nuevas y mejores técnicas usadas para poder evitar este tipo de errores y se muestra un casoclínico del manejo de un molar superior con una curvatura de 40 grados que fue preparado y obturado exitosamentecon el sistema Protaper (Dentsply- maillefer.

  20. Variables socionutricionales de hogares mazahuas integrados por preescolares desnutridos con madres con obesidad y sin obesidad

    OpenAIRE

    Viridiana Vanessa Conzuelo-González

    2009-01-01

    El primer objetivo fue conocer cuántos menores de cinco años con diferentes grados de desnutrición tienen una madre con sobrepeso/obesidad/ en una comunidad indígena que vive en extrema pobreza y bajo condiciones de migración masculina internacional. El segundo fue comparar tres variables socionutricionales (ingreso familiar, educación de la madre y adecuación nutrimental de la dieta diaria) entre estos hogares y los hogares con desnutrición infantil y madres sin obesidad. Se realizó un estud...