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Sample records for con ige elevada

  1. Niños con IgE elevada en sangre del cordón umbilical: seguimiento de 1 a 6 años

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    Dania Emma Fabré Ortíz

    1996-12-01

    Full Text Available Los valores elevados de IgE en suero del cordón umbilical se señalan como predictivos de desarrollo de alergias en etapas tempranas de la vida. La lactancia materna, dietas hipoalérgicas y el control de los aeroalergenos son preventivos de la aparición de estas afecciones. Como parte del Programa de Prevención de Enfermedades Alérgicas de Cuba y con el objetivo de conocer el valor predictivo de la IgE y el efecto de las medidas preventivas sobre los niños en riesgo se siguieron 752 niños durante 1 a 6 años con IgE elevada al nacer y se les indicó dichas medidas. La IgE al nacer y hasta los 3 años fue mayor en los enfermos en forma significativa (p 0,0241. Enfermaron menos aquéllos que lactaron y cumplieron las medidas orientadas (p 0,0028 y 0,0140 respectivamente. Se evidenció el valor predictivo de la IgE y los beneficios de las medidas profilácticas.The elevated values of IgE in umbilical cord serum are marked as predictors of allergies development at early stages of life. Maternal lactation, hypoallergic diets, and the control of aeroallergens may prevent the appearance of these affections. As part of the Prevention Programme of Allergic Diseases in Cuba and with the objective of knowing the predictive value of IgE and the effect of the preventive measures on children at risk, 752 children with elevated IgE at birth were followed from 1 to 6 years and the above mentioned measures were indicated to them. IgE at birth and up to 3 years was markedly higher (p 0,0241 among the sick. Those who lactated and accomplished the preventive measures got sick in a less degree (p 0,0028 and 0,0140, respectively. The predictive value of IgE and the benefits of the prophylactic measures were demonstrated.

  2. Prevalencia de TSH neonatal elevada y factores perinatales relacionados con asfixia Bogotá 2012

    OpenAIRE

    Sánchez Moreno, Edwin Alexander; Rojas Ávila, Camilo Eduardo

    2013-01-01

    Introducción: la asfixia perinatal es la tercera causa de muerte en menores de 5 años. Las secuelas neurológicas suponen una carga importante para las familias y los sistemas de salud (1). Los estudios que relacionan el efecto de la asfixia perinatal sobre las hormonas tiroideas son escasos. El estudio sobre predictores de asfixia es un tema de investigación permanente. El objetivo principal fue determinar la prevalencia de TSH de cordón elevada y su relación con factores perinatales asociado...

  3. Plantas transgénicas con alto rendimiento en peso seco y almidón cuyos órganos de reserva presentan elevada textura, elevado contenido en almidón y elevado rendimiento en peso seco

    OpenAIRE

    Pozueta Romero, Javier; Alonso Casajús, Nora; Muñoz Pérez, Francisco José; Baroja-Fernández, Edurne

    2007-01-01

    Plantas transgénicas con alto rendimiento en peso seco y almidón cuyos órganos de reserva presentan elevada textura, elevado contenido en almidón y elevado rendimiento en peso seco. La presente invención proporciona plantas transgénicas con alto rendimiento en peso seco y almidón cuyos órganos de reserva presentan elevada textura, elevado contenido en almidón y elevado rendimiento en peso seco.

  4. Elevada frecuencia de neuropatía periférica en pacientes con Diabetes mellitus tipo 2 de un hospital general de Lima-Perú

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    Ray Ticse

    2013-04-01

    Full Text Available Objetivos: Determinar la frecuencia de neuropatía periférica en pacientes con diabetes mellitus tipo 2 y la correlación entre la evaluación clínica con el estudio de la velocidad de conducción nerviosa (VCN. Material y métodos: Estudio descriptivo, transversal. Se evaluaron 62 pacientes con diagnóstico de diabetes mellitus tipo 2. Se utilizó el Michigan Neuropathy Screening Instrument (MNSI, el Michigan Diabetic Neuropatic Score (MDNS y el estudio de VCN. Además se describieron las variables demográficas, antropométricas y de laboratorio. Resultados: La frecuencia de neuropatía periférica fue 96,8% según la VCN y 45% según el MNSI. La correlación entre el número de nervios afectados según VCN y el score MDNS fue moderada (Spearman r=0,59; p<0,001. Conclusiones: Existe una elevada frecuencia de neuropatía periférica en los pacientes diabéticos evaluados y no hubo buena correlación entre VCN y el score MDNS.

  5. Estandarización de una prueba de ELISA para detectar anticuerpos IgE en pacientes con equinococosis quistica y su utilidad en el diagnóstico y seguimiento de pacientes tratados con albendazol: reporte preliminar

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    Herman Vildózola

    2012-01-01

    Full Text Available Objetivos: Determinar las diluciones y concentraciones optimas de una prueba de ELISA para detectar anticuerpos IgE, así como su sensibilidad, espécificidad y valor predictivo en pacientes con equinococosis quistica. Analizar si los niveles de anticuerpos IgE especificos se correlacionan con la respuesta al tratamiento médico con albendazol en un periodo mayor a un año de finalizado el tratamiento. Diseño: Estudio cuasiexperimental con grupo control. Institución: Instituto de Medicina Tropical Daniel A. Carrión, Facultad de Medicina, UNMSM, Lima, Perú. Material de estudio: Prueba de ELISA para anticuerpo IgE. Intervenciones: Estandarizacion de la prueba de ELISA para anticuerpo IgE y diseño preexperimento con preprueba y posprueba en un solo grupo, para evaluar su valor en el diagnóstico y seguimiento postratamiento de pacientes con quiste hidatidico hepatico tratados con albendazol. Para la estandarizacion de la prueba de ELISA, se utilizó suero de cinco pacientes con diagnóstico clínico de equinococosis quistica, y la sensibilidad y especificidad de la prueba se usó suero de 30 pacientes aparentemente sanos. Para determinar las reacciones cruzadas, se utilizó 16 muestras de suero de pacientes con otras helmintiasis (ascariasis, strongiloidiasis, toxocariosis, trichuriasis, himenolepiasis, cisticercosis y teniasis. Para el diagnóstico y seguimiento postratamiento de equinocococosis quística, se utilizó el suero de 17 pacientes. Principales medidas de resultados: Sensibilidad y especificidad de prueba estandarizada de ELISA para detectar anticuerpos IgE. Resultados: La prueba estandarizada de ELISA para detectar anticuerpos IgE tuvo una sensibilidad de 95,6% y una especificidad de 100%. En los pacientes con quiste hidatídico hepático considerados curados, uniformemente disminuyeron los niveles de anticuerpo tipo IgE hasta la negativización. Se obtuvo elevación de los niveles de IgE en los pacientes que presentaron

  6. Smoky indoor cooking fires are associated with elevated hemoglobin concentration in iron-deficient women Asocian el fuego con humo para cocinar dentro de la vivienda con concentraciones elevadas de hemoglobina en mujeres con deficiencia de hierro

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    Lynnette M. Neufeld

    2004-02-01

    combustión de materiales bioorgánicos utilizados como combustible, tales como la madera y los desechos agrícolas, está asociada con un mayor riesgo de padecer problemas respiratorios. Su efecto sobre otros aspectos de la salud, como el crecimiento fetal, no ha sido aún documentado adecuadamente. El objetivo del presente estudio, realizado en mujeres que utilizan combustible bioorgánico para cocinar en el interior de sus viviendas, fue determinar si el empleo de fuego "con humo" se asocia con una mayor concentración de hemoglobina en comparación con el uso de fogones "sin humo," es decir, cocinas diseñadas para reducir el nivel de contaminación del aire en el interior de las viviendas. Esta investigación es parte de una serie de estudios preliminares realizados para determinar la factibilidad y los posibles beneficios para la salud de una intervención aleatorizada dirigida a las cocinas, con el fin de reducir la contaminación del aire doméstico provocada por el empleo de combustibles bioorgánicos para cocinar. MÉTODOS: Entre marzo y agosto de 1994 se realizó un estudio observacional transversal en mujeres indígenas que utilizaban combustible bioorgánico y cocinaban en el interior de sus viviendas en comunidades rurales de las zonas montañosas de Guatemala. Se estudió a 89 mujeres que empleaban fogones sin humo (grupo no expuesto y 185 mujeres de las mismas comunidades que empleaban fogones que generaban humo (grupo expuesto. Ninguna estaba embarazada. Se tomaron muestras de sangre venosa y se determinaron las concentraciones de hemoglobina y de ferritina. Mediante análisis de regresión lineal múltiple se investigó la relación entre la exposición (fogones sin humo o fogones con humo y la concentración de hemoglobina, y se hicieron ajustes por posibles factores de confusión. RESULTADOS: No se encontró que la exposición al factor estudiado (fogones sin humo o con humo tuviera algún efecto en la concentración de hemoglobina, ya fuera mediante an

  7. Soil formation in high elevation and permafrost areas in the Qinghai Plateau (China Edafogénesis en zonas elevadas y con permafrost en el Plateau Qunghai (China Formação do solo em áreas de elevada altitude e em ambientes"permafrost" do planalto de Qinghai Plateau (China

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    Joselito Arocena

    2012-07-01

    Full Text Available

    Soil systems continuously gain and lose matter and energy even in areas where climate is too harsh to support the higher plants such as in high elevations and in permafrost environments. The purpose of this paper was to elucidate soil formation at 4 700 m asl in the Kunlun Mountains of the Qinghai (Tibet Plateau, China. We collected twenty-six samples from three pedons developed on carbonate-rich slate and quartzitic-sandstone materials. The samples were analyzed for routine physical, chemical and mineralogical properties. Our results indicate that soil-forming processes are barely taking place in the study site as shown by pH > 7.0 reflecting limited decarbonation, biological activity (< 1.0% total C and < 0.05% total N, podzolization (<1 g Fep+Alp kg-1 soil and lessivage. The relative amounts of mica and kaolinite show no apparent change with depth suggesting minimal transformation and translocation of phyllosilicates. The most noticeable mineral transformation resulted in micaceous “flakes” and feldspathic “fragments” possibly due to thermal stress. Calcite is also observed coating the slate fragments. The soils are classified as Cryosols in the World Reference Base system and Gelisols in the Soil Taxonomy with ochric epipedon as the only diagnostic horizon indicating recent development of soils due to cold and arid environment. The results of this study show that soil-forming processes in harsh environments are manifested in the mechanical breakdown of minerals such as mica and feldspars.

    Los sistemas edáficos ganan y pierden continuamente materia y energía incluso en zonas donde el clima es demasiado severo como para permitir el desarrollo de plantas superiores, como son las zonas de elevada altitud o los ambientes con permafrost. El objetivo de este trabajo es analizar la formación del suelo a una altitud de 4 700 m en las Monta

  8. Experiência com transplante cardíaco heterotópico em pacientes com resistência pulmonar elevada: seguimento tardio Experiencia con trasplante cardíaco heterotópico en pacientes con resistencia pulmonar elevada: seguimiento tardío Experience with heterotopic heart transplantation in patients with elevated pulmonary vascular resistance: late follow-up

    Directory of Open Access Journals (Sweden)

    Jose Henrique Andrade Vila

    2010-02-01

    selecionados.FUNDAMENTO: En los últimos años el número de artículos sobre trasplante cardíaco heterotópico y, en particular, del seguimiento a largo plazo de estos pacientes, ha sido escaso en la literatura, inclusive internacional, lo que llevó al presente relato. OBJETIVO: Relatar la experiencia clínica inicial y la evolución tardía de cuatro pacientes sometidos a trasplante cardíaco heterotópico, su indicación y principales complicaciones. MÉTODOS: Las cirugías se realizaron entre 1992 y 2001, y la indicación de trasplante heterotópico, en todas, fue mediante RVP, variable de 4,8 UW; a 6.5 UW, con gradiente transpulmonar superior a 15 mmHg. En el tercer paciente, se realizó una anastomosis directa entre las arterias pulmonares sin empleo de tubo prostético, y, en el corazón nativo, se realizó una valvuloplastia mitral y aneurismectomía de VI. El esquema inmunosupresor inmediato fue doble, con ciclosporina y azatioprina en los tres primeros pacientes y ciclosporina y micofenolato mofetil en el cuarto paciente. RESULTADOS: Un óbito inmediato por falla del injerto, un óbito luego de dos años y medio por endocarditis en trombo intraventricular en el corazón nativo, y un tercer óbito seis años después del trasplante, por complicaciones postoperatorias de una cirugía en la válvula aórtica del corazón nativo. El restante, 15 años después del trasplante, se encuentra bien, en clase funcional II (NYHA, seis años después de una oclusión quirúrgica de la válvula aórtica del corazón nativo. CONCLUSIÓN: El trasplante cardíaco heterotópico es un procedimiento con resultado inferior al trasplante cardíaco ortotópico, por presentar mayor RVP. Los trombos intraventriculares en el corazón nativo, que exige anticoagulación prolongada, así como las complicaciones de válvula aórtica, también en el corazón nativo, pueden exigir tratamiento quirúrgico. Sin embargo, en un paciente, la sobrevida de 15 años mostró la eficacia a largo plazo de

  9. Clareo con selección de árboles de porvenir sobre regenerado post-incendio de elevada densidad de pino carrasco en el Parque Regional del Sureste.

    OpenAIRE

    Orduña Rubio, Berta

    2017-01-01

    Se presenta una experiencia sobre una masa de pino carrasco de 10 años de edad y elevada densidad (más de 10.000 pies/ha), en estado de monte bravo a bajo latizal, originada tras un incendio ocurrido en una repoblación (monte “Casa Eulogio”; Rivas-Vaciamadrid, Madrid). Se seleccionaron 400 pies/ha de porvenir, apeándose todos los pies vecinos en un radio de 1 ó 2 m para favorecer su desarrollo. Todo ello, en un contexto de restricciones económicas que obliga a buscar fórmulas que optimicen el...

  10. Conglomeración de precursores de tensión arterial elevada en adolescentes

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    Carlos M. Arango

    2014-09-01

    Conclusión: La conglomeración de dos o más precursores se asoció de manera significativa con la tensión arterial elevada, hallazgos que indican que se requieren intervenciones para reducir la presencia y acumulación de los precursores estudiados y así prevenir la adquisición de cifras de tensión arterial elevada en los adolescentes de Montería.

  11. Cintas, capas o láminas superconductoras y su método de fabricación a partir de disoluciones precursoras sin flúor con elevadas velocidades de crecimiento

    OpenAIRE

    Obradors Berenguer, Xavier; Puig Molina, Teresa; Ricart Miró, Susana; Soler Bru, Laia

    2014-01-01

    [ES] La presente invención se refiere a unas cintas, capas o láminas superconductoras con una densidad de corriente crítica y a su método de fabricación basada en la deposición química de sales metalorgánicas sin flúor mediante impresión de chorro de tinta bajos unas condiciones de rampas de temperatura y presión específicas que hacen que la velocidad de fabricación sea de hasta 1000 nm/s. Además, la presente invención se refiere al uso de dichas cintas, capas o láminas como componentes super...

  12. Intranasal sensitization with Blomia Tropicalis antigens induces allergic responses in mice characterized by elevated antigen-specific and non-specific serum ige and peripheral blood eosinophil counts Sensibilização intranasal com antígenos de Blomia tropicalis induz respostas alérgicas em camundos caracterizadas pela elevada contagem de soro IgE antígeno-específico e não específico e de eosinófilos no sangue periférico

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    Fumiko Takeda

    2004-02-01

    Full Text Available In order to evaluate the potential allergenicity of Blomia tropicalis (Bt antigen, IgE production of both specific and non-specific for Bt antigen was monitored in BALB/c mice after exposure to the antigen by nasal route. It was evidenced that B. tropicalis contains a functional allergen in its components. The allergenic components, however, when administered intranasally without any adjuvant, did not function to induce IgE response within a short period. On the other hand, intranasal inoculation of Bt antigens augmented serum IgE responses in mice pretreated by a subcutaneous priming injection of the same antigens. Inoculation of Bt antigen without subcutaneous priming injections induced IgE antibody production only when the antigen was continuously administered for a long period of over 24 weeks. Even when the priming injection was absent, the Bt antigen inoculated with cholera toxin (CT as a mucosal adjuvant also significantly augmented the Bt antigen-specific IgE responses depending on the dose of CT co-administered. The present study also demonstrated that Bt antigen/CT-inoculated mice showed increased non-specific serum IgE level and peripheral blood eosinophil rates without noticeable elevations of the total leukocyte counts. The immunoblot analysis demonstrated 5 main antigenic components reactive to IgE antibodies induced. These components at about 44-64 kDa position were considered to be an important candidate antigen for diagnosis of the mite-related allergy.Para avaliar a capacidade alergizante do antígeno da Blomia tropicalis (Bt a produção de IgE específica e não específica a antígeno Bt foi monitorada em camundongos BALB/c após exposição ao antígeno por via nasal. Foi evidenciado que Bt contem um alérgeno funcional em seus componentes. Os componentes alergênicos entretanto, quando administrados por via intra-nasal, sem qualquer adjuvante, não induzem resposta IgE durante um pequeno período. Por outro lado, a inocula

  13. Carretera elevada, en Hannóver

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    Wittfoht, Hans

    1965-02-01

    Full Text Available In the new planning of Hannover, a new elevated roadway has been provided, to connect the Königsworther place with the Westtangente motor road. This elevated road is built on a prestressed concrete palisade which is 360 m long and forms 17 spans, whose mean length is 22 m. The road runs on a curve, and there are two dilation joints, separating the structure into three sections. The deck is prestressed, and rests on portal frames, with inclined columns, whose top cross members, also prestressed, cantilever 1.24 ms on each side of the columns. The longitudinal prestressing cables have been given a load of 116 tons per cable, and the transversal ones, spaced every 0.75 m, carry a load of 57 tons per cable. This project was finished very rapidly, thanks to the magnificent auxiliary facilities. The concrete was transported from the mixing plant to the site, about 200 m away, by pneumatic pumps. A special feature of this project is that the lower ends of the columns rest on the foundations by means of sets of roller bearings, which are carefully greased.En la nueva ordenación urbana de la ciudad de Hannover (Alemania, se ha incluido un acceso elevado, especie de puente, que unirá la plaza de Königsworther con la autopista Westtangente. Este acceso es de hormigón pretensado y tiene una longitud total de 360 m, subdividida en 17 tramos de unos 22 m de luz media. Como el trazado tiene un eje en curva, se han previsto dos juntas que dividen el acceso en tres trozos. El tablero es de hormigón pretensado y se apoya sobre una serie de pórticos, con montantes inclinados, cuyos cabezales, también pretensados, tienen 1,24 m de canto y se prolongan en voladizo por ambos lados. Los cables del pretensado longitudinal se han tesado a razón de 116 t cada uno y los del pretensado transversal, especiados a 0,75 m, a 57 t por cable. Esta obra se terminó con gran celeridad, gracias a las magníficas instalaciones auxiliares dispuestas. El hormigón se

  14. Resource Allocation and Time Utilization in IGE and Non-IGE Schools. Technical Paper No. 410.

    Science.gov (United States)

    Rossmiller, Richard A.; Geske, Terry G.

    This study addressed two basic questions; (1) Do individually guided education (IGE) schools cost more or exhibit different expenditure patterns than non-IGE schools? (2) Do instructional personnel in IGE schools allocate their time differently than instructional personnel in non-IGE schools? Data were obtained from a random sample of 41 IGE…

  15. NASA-IGES Translator and Viewer

    Science.gov (United States)

    Chou, Jin J.; Logan, Michael A.

    1995-01-01

    NASA-IGES Translator (NIGEStranslator) is a batch program that translates a general IGES (Initial Graphics Exchange Specification) file to a NASA-IGES-Nurbs-Only (NINO) file. IGES is the most popular geometry exchange standard among Computer Aided Geometric Design (CAD) systems. NINO format is a subset of IGES, implementing the simple and yet the most popular NURBS (Non-Uniform Rational B-Splines) representation. NIGEStranslator converts a complex IGES file to the simpler NINO file to simplify the tasks of CFD grid generation for models in CAD format. The NASA-IGES Viewer (NIGESview) is an Open-Inventor-based, highly interactive viewer/ editor for NINO files. Geometry in the IGES files can be viewed, copied, transformed, deleted, and inquired. Users can use NIGEStranslator to translate IGES files from CAD systems to NINO files. The geometry then can be examined with NIGESview. Extraneous geometries can be interactively removed, and the cleaned model can be written to an IGES file, ready to be used in grid generation.

  16. The synthesis of the insecticides Aldrin and Dieldrin labelled with carbon-14 at high specific activity; Synthese des insecticides aldrine et dieldrine marques au carbone-14 de haute activite specifique; Sintez insektitsidnogo aldrina i dildrina, mechennykh uglerodom-14 pri vysokoj udel'noj aktivnosti; Sintesis de los insecticidas aldrin y dieldrin de elevada actividad especifica marcados con carbono-14

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, Daniel J; Kilner, A Edward [Radiochemical Centre, UKAEA, Amersham, Bucks (United Kingdom)

    1962-03-15

    nombre comercial que la compania Shell Chemicals dio al 1, 2, 3, 4, 10, 10-hexacloro-1, 4, 4a, 5, 8, 8a-hexabidro-exo-1, 4-endo-5, 8-dimetanonaftaleno. El acetileno-1, 2-{sup 14}C se convierte primero en tetracloroetano y despues en tricloroetileno, y este se condensa con tetracloruro de carbono, por la reaccion de Prins, en presencia de cloruro de aluminio, para dar octaclorociclopenteno. Por decloracion se obtiene hexaclorociclopentadieno que, por una sintesis de Diels-Alder, da biciclo (2, 2, 1) hepta-2, 5-dieno para llegar al aldrin-{sup 14}C con un rendimiento del 12% referido al carbonato de bario. La oxidacion del aldrin da el 6, 7-epoxido, denominado dieldrin, con un rendimiento del 87%. Los autores describen la separacion del octaclorociclopenteno del producto bruto de la reaccion de Prins por cromatografia gas-liquido, y la separacion del aldrin y del dieldrin en pequena escala, por cromatografia sobre el papel, de fase invertida. (author) [Russian] Aldrin - kommercheskoe nazvanie, dannoe SHellom Kemikalom dlya 1 ,2, 3, 4, 10, 10-geksakhloro-1, 4, 4a, 5, 8, 8a-geksagidro ehkso-1,4-ehndo-5,8-dimetanonaftalina. Atsetilen-1,2-C{sup 14} uspeshno prevrashchaetsya v tetrakhloroehtilen i trikhloroehtilen i kondensiruetsya s uglerodistym tetrakhloridom v reaktsii Prinsa v prisutstvii alyuminievogo khlorida v oktakhlorotsiklopentan. Dekhlorizatsiya privodit k geksakhlorotsiklopentadinu, kotoryj dobavlyaetsya v vide Dils-Aldera k bitsiklo (2, 2, 1) gepta-2,5-dinu dlya polucheniya iz barievogo karbonata 12% aldrina-C{sup 14}. Okislenie aldrina privodit k obrazovaniyu ehloksida 6,7 i dildrina v razmere 87%. V dokumente soderzhitsya otchet ob otdelenii oktokhlorotsiklopentana ot syrogo produkta reaktsii Prinsa putem gazozhidkoj khromatografii i ob otdelenii aldrina i dildrina v nebol'shom kolichestve putem obratnoj fazy bumazhnoj khromatografii. (author)

  17. Cord blood IgE. I. IgE screening in 2814 newborn children

    DEFF Research Database (Denmark)

    Hansen, L G; Høst, A; Halken, S

    1992-01-01

    Screening of total IgE in 2814 cord blood samples was analysed by Phadebas IgE PRIST in 2 1-year birth cohorts (1983-1984 and 1985-1986) in Denmark (n = 1189 + 1625). 48.6% of the sera contained less IgE than the detection limit 0.1 kU/l. Cord blood IgE values greater than or equal to 0.5 kU/l we...

  18. Desde la elevada meseta mexicana: con una larga-vista demográfica.

    Directory of Open Access Journals (Sweden)

    Raúl Benítez Zenteno

    2001-01-01

    Full Text Available En la segunda mitad del siglo XX, contrario a los discursos políticos y algunas teorías, en los países pobres el menor crecimiento demográfico inducido por las políticas internacionales no se reflejó en crecimiento económico y mejores condiciones de vida. Cuestión de la que se deriva la interrogante de cuál ha sido nuestra participación en la toma de decisiones orientadas a ampliar el bienestar. Posteriormente, se expone lo que han sido las áreas de investigación en materia de población y se realiza un balance sobre las "modas" que han llegado del exterior, señalando que, en general, el saldo ha sido negativo, principalmente por su adopción acrítica, la falta de creatividad teórica propia y la poca profundidad en la explicación de interrelaciones entre los factores demográficos, socioeconómicos, políticos y culturales. Para concluir, se hace una revisión de la práctica demográfica en México, en la que resalta la falta de demógrafos bien formados, la persistencia de explicaciones reducidas, la poca colaboración grupal e interinstitucional, y la ausencia de recursos.

  19. JSI-124 inhibits IgE production in an IgE B cell line

    International Nuclear Information System (INIS)

    Cui, Lulu; Bi, Jiacheng; Yan, Dehong; Ye, Xiufeng; Zheng, Mingxing; Yu, Guang; Wan, Xiaochun

    2017-01-01

    IgE is a key effector molecule in atopic diseases; however, the regulation mechanisms of IgE production in IgE B cells remain poorly understood. In the present study, we demonstrate that JSI-124 (cucurbitacin I), a selective STAT3 inhibitor, selectively inhibits production of IgE by a human IgE B cell line, CRL-8033 cells, while does not affect the IgG production by IgG B cell lines. In the aspect of molecular mechanism, we found that Igλ, but not Ighe, gene expression was suppressed by JSI-124. The above effects of JSI-124 were not mediated by affecting cellular proliferation or apoptosis. Furthermore, multiple B cell differentiation-related genes expression was not significantly affected by JSI-124. Taken together, we demonstrate a potential strategy of therapeutically suppressing IgE production without affecting IgG production in atopic patients. - Highlights: • JSI-124 inhibits IgE production in an IgE B cell line, CRL-8033 cells. • JSI-124 does not affect IgG production by IgG B cell lines. • JSI-124 inhibits IgE production mainly by suppressing transcription of Igλ.

  20. Determination of IgE rheumatoid factor

    International Nuclear Information System (INIS)

    Herrmann, D.; Schlenvoigt, G.; Jaeger, L.

    1987-01-01

    A solid-phase radioimmunoassay and an enzyme-linked immunosorbent assay have been developed for the identification of IgE rheumatoid factor (IgE RF). For both, human IgG was used as antigen. Bound IgE RF was detected by means of commercially available rabbit anti-human IgE antiserum and 125 I-labelled sheep anti-rabbit IgG as well as monoclonal anti-human-ε-chain antibody and horse-radish peroxidase-labelled sheep anti-mouse IgG. The presence of IgM RF did not cause false positive results. Correlation in the results of both assays were significant, the reproducibility was very good. In 50.6% of 79 sera from patients with rheumatoid arthritis IgE RF has been detected with both or one of the methods. Only in 1 out of 12 seronegative rheumatoid arthritis sera IgE RF was identified. (author)

  1. ASOCIACIÓN ENTRE NIVELES SÉRICOS DE VITAMINA D, RESPUESTA IgE Y VARIANTES GENÉTICAS DEL GEN DEL RECEPTOR DE LA VITAMINA D EN NIÑOS OBESOS DEL CARIBE COLOMBIANO: UN ESTUDIO DE CASOS Y CONTROLES

    Directory of Open Access Journals (Sweden)

    Eduardo A. Egea Bermejo

    2016-07-01

    Full Text Available

    Introducción: Estudios previos han evaluado la relación entre vitamina D y atopía. En pacientes obesos es incierta esta asociación, sin embargo se acepta hoy que polimorfismos en el receptor de vitamina D pueden asociarse con obesidad. Actualmente son escasos los estudios que han buscado esta asociación en grupos en el litoral Caribe.

    Objetivo: Analizar la asociación entre los polimorfismos de los SNPs del receptor de vitamina D con la susceptibilidad para obesidad, su influencia en los niveles séricos de vitamina D e IgE en una población infantil.

    Metodología: Se realizó un estudio analítico de casos (n=120 y controles (n=182 con niños obesos entre los 5 y 17 años de edad, procedentes de la costa caribe colombiana. Los niveles séricos de 25(OH vitamina D e IgE Total se midieron mediante ELISA. Valores de p<0.05 fueron estadísticamente significativos.

    Resultados y conclusiones: Se encontró asociación entre niveles altos de vitamina-D y obesidad infantil. El 48,3% (n=58 de los casos presentaron niveles >100 ng/mL en comparación a un 0,5% (n = 1 de los controles. Ambos grupos presentaron altos niveles de IgE, (317,07±331,5 vs. 280,6±272.7; p = 0,786. Nuestros resultados no mostraron asociación alguna entre las variantes genéticas con los niveles séricos de vitamina-D con IgE total, como tampoco con obesidad. Todos los SNPs estuvieron en equilibrio Hardy-Weingber. Nuestros resultados no muestran asociación de deficiencia de Vitamina D con obesidad en este grupo poblacional, pero sí IgE elevada en la mayoría de la población, sin tener ninguna relación entre ellos. (Rho spearman: 0,023; p = 0,641.

    ASSOCIATION BETWEEN SERUM LEVELS OF VITAMIN D, IgE RESPONSE AND GENETIC VARIANTS OF THE GENE FOR VITAMIN D RECEPTOR IN OBESE CHILDREN IN THE COLOMBIAN CARIBBEAN

  2. [IgE myeloma. Laboratory typing difficulties].

    Science.gov (United States)

    Bovone, Nora S; Fuente, María Cristina; Gastiazoro, Ana María; Alfonso, Graciela; Freitas, María Josefina

    2014-01-01

    The IgE multiple myeloma is a rare neoplasm of plasma cell accounting for 0.01% of all plasma cell dyscrasias. They are generally of more aggressive development and to date there are no more than 50 cases published in current literature. Laboratory studies are, in these cases, essential for the classification of the monoclonal component in serum and urine. The aim of this presentation is to report a patient diagnosed with IgE myeloma and to point out that the laboratory difficulties noted in these rare cases can lead to an erroneous report.

  3. IgE antibodies in toxoplasmosis.

    Science.gov (United States)

    Matowicka-Karna, Joanna; Kemona, Halina

    2014-05-15

    Toxoplasmosis is a worldwide infection caused by the intracellular parasite Toxoplasma gondii. At least a third of the world human population is infected with the parasite, making it one of the most successful parasitic infections. Primary maternal infection may cause health-threatening sequelae for the fetus, or even cause death of the uterus. Reactivation of a latent infection in immune deficiency conditions such as AIDS and organ transplantation can cause fatal toxoplasmic encephalitis. Toxoplasmosis is a major cause of chorioretinitis, especially in individuals with impaired immune systems. In the acute phase, directly after invading the body, T. gondii begins to multiply rapidly. In the majority of cases acquired toxoplasmosis is asymptomatic. In the second week of infection, specific IgM antibodies are present in the blood. IgE antibodies appear at the same time, slightly preceding specific IgA antibodies. The concentration of IgE can be one of the parameters used for diagnosing an infection with T. gondii. Laboratory diagnosis, i.e. IgE and serologic assays, plays the main role in the diagnosis of congenital infection and assists in the confirmatory diagnosis of toxoplasmic encephalitis and ocular toxoplasmosis. This article is a review of IgE in toxoplasmosis.

  4. Methods of quantifying circulating IgE

    International Nuclear Information System (INIS)

    Merrett, T.G.; Merrett, J.

    1978-01-01

    Four radioimmunoassay techniques, two conventional and two sandwich, have been used to measure circulating IgE levels in 100 sera. The test sera had IgE levels ranging from 1.0 to 20,000 u/ml, and each was measured at five dilutions, ranging from three-fold to 400-fold. The same IgE standards were used throughout, and the optimal range for each assay was determined by assessing data for quality control sera and the WHO standard 69/204. To be of general use in the United Kingdom an IgE test must measure accurately levels as low as 20-30 u IgE/ml. The Phadebas RIST method failed to meet this criterion, and of the remaining tests the double antibody method had the most useful operating range and produced the most reliable results. However, the double antibody method is not available commercially and so, for the majority of laboratories, the Phadebas PRIST technique should be the method chosen. (author)

  5. The determination of sulphur in materials of high neutron absorption cross-section by fast-neutron activation analysis; Determination du soufre dans les matieres de forte section efficace d'absorption neutronique, au moyen d'une analyse par activation avec des neutrons rapides; Opredelenie sery v materialakh s bol'shim secheniem pogloshcheniya nejtronov metodom aktivatsionnogo analiza bystrykh nejtronov; Determinacion del azufre en sustancias de elevada seccion eficaz de absorcion neutronica mediante analisis por activacion con neutrones rapidos

    Energy Technology Data Exchange (ETDEWEB)

    Gibbons, D; Simpson, H [Wantage Research Laboratory (A.E.R.E.), Wantage, Berks. (United Kingdom)

    1962-01-15

    The production of radiophosphorus by the fast neutron (n, p) reaction on sulphur is used for the determination of sulphur by activation analysis. Using this technique it is possible to determine sulphur in materials of high thermal-neutron absorption cross-section because the absorption cross-sections for fast neutrons are, in general, much lower than those for slow neutrons, so that self-shielding errors are considerably reduced. Interference due to the slow neutron (n, {gamma}) reaction on neutral phosphorus is eliminated by a double irradiation technique involving irradiation in regions of differing slow/fast flux-ratios. The method has been applied to the determination of sulphur in chromium and arsenic. (author) [French] La production de radiophosphore par reaction (n, p) de neutrons rapides sur le soufre est utilisee pour la determination du soufre au moyen d'une analyse par activation. Cette methode permet la determination du soufre dans les matieres de forte section efficace d'absorption des neutrons thermiques; en effet, les sections efficaces d'absorption des neutrons rapides sont, d'une maniere generale, beaucoup plus faibles que pour les neutrons lents, de sorte que les erreurs d'autoprotection se trouvent considerablement reduites. L'interference due a la reaction (n, {gamma}) des neutrons lents sur le phosphore naturel est eliminee par une double irradiation, qui implique une irradiation dans les regions ou le rapport du flux de neutrons lents au flux de neutrons rapides a des valeurs differentes. La methode a ete appliquee a la determination du soufre dans le chrome et l'arsenic. (author) [Spanish] La formacion de fosforo radiactivo por la accion de los neutrones rapidos sobre el azufre, reaccion (n, p), puede servir para determinar este elemento mediante analisis por activacion. El empleo de esta tecnica permite efectuar determinaciones de azufre en materiales de elevada seccion eficaz de absorcion de neutrones termicos porque, en general, las

  6. Transfer of maternal IgE can be a common cause of increased IgE levels in cord blood

    DEFF Research Database (Denmark)

    Bønnelykke, Klaus; Pipper, Christian Bressen; Bisgaard, Hans

    2010-01-01

    IgE in cord blood is thought to be a product of the fetus. A high level of total IgE is therefore used as a measure of atopic propensity in the newborn. We recently found strong evidence that allergen-specific IgE in cord blood was the result of transfer of maternal IgE to fetal blood or cord blood...

  7. Role and Redirection of IgE against Cancer

    Directory of Open Access Journals (Sweden)

    Elisa A. Nigro

    2013-05-01

    Full Text Available IgE is a highly elusive antibody class, yet a tremendously powerful elicitor of immune reactions. Despite huge efforts spent on the characterization and understanding of the IgE system many questions remain either unanswered or only marginally addressed. One above all relates to the role of IgE. A common doubt is based on whether IgE mode of action should only be relegated to anti-parasite immunity and allergic manifestations. In search for a hidden role of IgE, reports from several laboratories are described herein in which a natural IgE link to cancer or the experimental redirection of IgE against cancer have been investigated. Epidemiological and investigational studies are trying to elucidate a possible direct intervention of endogenous IgE against cancer, raising thus far no definitive evidence. Conversely, experimental approaches implementing several strategies and engineered IgE formats built up a series of convincing results indicating that cancer might be tackled by the effector functions of this immunoglobulin class. Because of its peculiar immune features, IgE may present a superior anti-tumor performance as compared to IgG. However, extreme care should be taken on how IgE-based anti-tumor approaches should be devised. Overall, IgE appears as a promising resource, likely destined to enrich the anti-cancer arsenal.

  8. Nueve casos del sindrome HELLP (hemolisis, enzimas hepaticas Elevadas y plaquetopenia

    Directory of Open Access Journals (Sweden)

    M. F. Capellino

    2003-10-01

    Full Text Available El síndrome HELLP (hemólisis, enzimas hepáticas elevadas y plaquetopenia descripto por Weinstein en 1982, se asocia a alta morbi-mortalidad materna y perinatal. Se evaluaron retrospectivamente las pacientes que presentaron síndrome HELLP entre marzo 1998 y marzo 2001 en el Hospital Privado de Córdoba. Se identificaron nueve pacientes con Síndrome HELLP (incidencia 2.3‰. La edad media de las pacientes fue 24.5 años (15-36 con una edad gestacional media de 34.5 semanas (29-40. Cinco pacientes fueron nulíparas. El síntoma principal fue epigastralgia (77.7%. El parto fue por cesárea en siete pacientes y tres tuvieron síndrome HELLP post-parto. Tres pacientes presentaron hipertensión de difícil manejo, una eclampsia y otra coagulación intravascular diseminada, insuficiencia renal aguda y muerte. Tres requirieron transfusión de derivados sanguíneos y el promedio de internación fue 4.4 días. Seis (75% neonatos fueron pretérmino. El peso medio de nacimiento fue 2030 gramos (736-3200. Cuatro recién nacidos tuvieron un score de Apgar menor de 7 al minuto y todos mayor de 7 a los cinco minutos. Tres neonatos presentaron trastornos alimentarios, uno hipoglucemia y otro enfermedad de membrana hialina, ductus arterioso permeable, sepsis y plaquetopenia. Debido a la morbi-mortalidad materna y perinatal el síndrome HELLP requiere atención en un centro de alta complejidad mediante un equipo multidisciplinario.HELLP syndrome (Hemolysis, Elevated Liver Enzymes and Low Platelets was described by Weinstein in 1982. It has a high maternal and perinatal morbi-mortality rate. We undertook this study to evaluate perinatal outcome in patients with HELLP syndrome. Patients with HELLP syndrome were identified in a retrospective study between March 1998 and March 2001 at the Hospital Privado de Córdoba. Maternal and neonatal variables were analized. Nine patients with HELLP syndrome were identified (incidence 2.3‰. Mean maternal age was 24.5 (15

  9. Relações pedomorfogeológicas nas chapadas elevadas do Distrito Federal Pedomorphogeological relations in the chapadas elevadas of the Distrito Federal, Brazil

    Directory of Open Access Journals (Sweden)

    Inara Oliveira Barbosa

    2009-10-01

    Full Text Available A caracterização pedológica e o entendimento das relações entre pedologia, geologia e geomorfologia são importantes para a compreensão da distribuição dos solos numa paisagem. O objetivo deste estudo foi avaliar as relações pedomorfogeológicas na região das Chapadas Elevadas do Distrito Federal (DF, mediante caracterização química, física, mineralógica e geoquímica dos solos de ocorrência nesse compartimento da paisagem. Foram selecionadas duas topossequências representativas da distribuição pedológica nas Chapadas Elevadas do DF, cujos solos foram formados a partir de rochas metassedimentares do Grupo Paranoá, representados por Latossolos Vermelhos (LV, Latossolos Vermelho-Amarelos (LVA e Cambissolos (C. Os Latossolos das duas topossequências apresentaram a maioria dos atributos físicos, químicos e mineralógicos semelhantes. A variação da cor nesses Latossolos é proveniente da mineralogia diferenciada dos óxidos de Fe - hematita predominante nos LV e goethita nos LVA. No entanto, as análises por meio de ICP-AES apresentaram teores de Fe2O3 similares nos Latossolos, demonstrando material de origem (rochas metassedimentares de composição geoquímica semelhante. A formação da goethita nos LVA foi considerada dependente da sua posição geomorfológica de desenvolvimento, nas bordas das chapadas, onde a oscilação do lençol freático proporcionou a formação de horizonte litoplíntico, com consequente deficiência das condições de drenagem. Os Cambissolos apresentaram-se quimicamente semelhantes aos Latossolos em razão do material de origem, que são rochas metassedimentares já pré-intemperizadas. O estudo das relações pedomorfogeológicas permitiu constatar que a distribuição dos solos nas Chapadas Elevadas do Distrito Federal é condicionada pela evolução geomorfológica e pela geologia da região.Detailed studies of pedologic characterization, as well as of relationships between pedology

  10. Tear and serum IgE concentrations by Tandem-R IgE immunoradiometric assay in allergic patients

    International Nuclear Information System (INIS)

    Insler, M.S.; Lim, J.M.; Queng, J.T.; Wanissorn, C.; McGovern, J.P.

    1987-01-01

    The authors studied a population of 39 allergic and 15 nonallergic patients, and determined their tear and serum IgE concentrations. Samples of tear and serum were tested for IgE by the Tandem-R immunoradiometric assay, which uses monoclonal antibody to produce a specific assay for IgE. The serum IgE levels in the study group showed a range from 23,280 to 16 IU/ml compared with controls of 72 to 2 IU/ml. Tear IgE in the study group varied from 159 IU/ml to less than 1 IU/ml compared with controls of 8 IU/ml to less than 1 IU/ml. A statistically significant correlation between tear and serum IgE exists in the allergic patients with eye symptoms. It also exists when serum IgE was greater than 100 IU/ml, the tear IgE greater than 4 IU/ml, or when both the serum IgE was greater than 100 IU/ml and the tear IgE greater than 4 IU/ml

  11. Local IgE production in nonatopic nasal polyposis.

    LENUS (Irish Health Repository)

    Sheahan, Patrick

    2012-02-01

    INTRODUCTION: Chronic rhinosinusitis with nasal polyposis (CRSwNP) represents an eosinophilic T-helper 2 inflammatory response. Local production of IgE within nasal polyps (NPs) has been demonstrated, suggesting a role for local IgE in the pathogenesis of NP in atopic CRS patients. We hypothesized that local IgE specific to inhalant allergens may also play a role in the genesis of NP in nonatopic CRS patients. METHODS: Sinus and inferior turbinate tissue was obtained from nonatopic CRSwNP patients (n = 7), chronic rhinosinusitis without nasal polyps (CRSsNP) patients (n = 15), and healthy controls (n = 9) at the time of surgery. ImmunoCAP analysis (Phadia AB, Portage, MI) for 14 common inhalant antigens was performed on tissue homogenates to determine the antigen-specific response. RESULTS: Total IgE levels did not differ in sinus or turbinate tissue between CRSwNP, CRSsNP, or control patients. CRSwNP sinus tissue had higher levels of specific IgE for cockroach and plantain (p = .03) than other groups and elevated Alternaria IgE levels when compared with CRSsNP sinus tissue (p < .05). No significant differences were found for any of the other antigen-specific IgE levels. Fifty-seven percent of CRSwNP polyps demonstrated a polyclonal IgE response, whereas the other 43% had no demonstrable antigen-specific IgE. In contrast, only 17% of CRSsNP patients demonstrated a polyclonal response within sinus tissue, whereas 67% had no detectable antigen-specific IgE. There was no significant difference in levels of IgE in inferior turbinate tissue between the groups (p > .05). CONCLUSIONS: Localized mucosal IgE specific to common inhalant allergens appears to play a role in a subset of CRSwNP patients without evidence of systemic atopy.

  12. NOSOTROPIC SUBSTANTIATION OF ANTI IgE ANIBODY THERAPY

    Directory of Open Access Journals (Sweden)

    Yu.G. Levina

    2008-01-01

    Full Text Available This article studies the specifics of allergic diseases pathogenesis. Such common allergic diseases as bronchial asthma, allergic rhinitis and atopic dermatitis are conditioned by processes based on increase of immunoglobulin e synthesis. Omalizunab, which is a recombinant humanized monoclone anti-Ige antibody, prevents Ige fixing to membrane receptors of mast cells and significantly reduces the level of immunoglobulin e circulating in blood.Key words: anti-Ige antibody, omalizumab, allergic diseases, Bronchial asthma, children.

  13. Experiencias en Cuba de la producción de piezas fundidas de fundición gris esferoidal (FGE, un material de elevadas cualidades y propiedades.

    Directory of Open Access Journals (Sweden)

    Francisco Mondelo García

    2005-09-01

    Full Text Available El trabajo expone alternativas posibles de acceder a fabricar fundiciones grises esferoidales(FGE, así como conocimientos, experiencias y métodos empleados en su producción en Cuba,contando por lo tanto con un material de elevadas cualidades y propiedades tecnológicas enexplotación. Se dan materiales básicos utilizados en los procesos de obtención de la FGE, locual incluye el proceso de recarburación de estas aleaciones ferrosas, más las operaciones denodulización e inoculación, junto a los controles bases en sus operaciones fundamentales, parafabricar piezas con la calidad y certificación requeridas. Se tienen resultados experimentalesproductivos de estas fundiciones nodulares, con marcas de diversas estructuras, propiedadesmecánicas y composiciones. Se valora además resultados de la sustitución de piezas deaceros por FGE con posibilidades de obtener, por tratamiento térmico los hierrosaustemperados (ADI y elementos económicos comparativos de la factibilidad de fabricación dela fundición sintética en piezas FGE con chatarras de aceros básicamente y recarburantesnacionales de carbón vegetal, como variante alternativa.

  14. Influencia de elevadas temperaturas en la adherencia de barras de fibra de carbono con el hormigón

    OpenAIRE

    Cos-Gayón López, Fernando José

    2017-01-01

    The need to mitigate the effects of corrosion on the reinforced concrete structure, and to do so permanently, has led to the proposal of replacing steel bars with other materials that respond adequately to the presence of water without developing corrosive processes. These reinforcements shall ensure the safety and service conditions of the structure over a lifetime and in very adverse climatic conditions. One of the materials being proposed, and de facto already replacing in some place...

  15. Pavimentos con un reducido nivel de ruido superficial y una elevada adherencia : Resumen de la experiencia francesa

    OpenAIRE

    BROSSEAUD, Y; ANFOSSO LEDEE, F

    2006-01-01

    Noise forms one of the major annoyances the general public has to suffer, especially in large urban agglomerations. As a result of the swift and growing urban development and the growth of urban road infrastructure, for over 20 years road engineers have been carrying out research with a view to reducing rolling tyre pavement noise, which constitutes the greatest source of noise as from vehicle speeds of over 50 km/h. The French authorities (the LCPC in conjunction with the network of road lab...

  16. Indicadores antropométricos como preditores de pressão arterial elevada em adolescentes

    Directory of Open Access Journals (Sweden)

    Carmem Cristina Beck

    2011-02-01

    Full Text Available FUNDAMENTO: A hipertensão arterial está relacionada ao incremento da gordura corporal, a qual pode ser avaliada por meio de indicadores antropométricos. OBJETIVO: Determinar o poder preditivo de indicadores antropométricos e estabelecer seus pontos de corte como discriminadores de pressão arterial elevada. MÉTODOS: Estudo transversal realizado com uma amostra de 660 adolescentes de 14 a 19 anos sendo 51,9% moças. Foram considerados os seguintes indicadores antropométricos: índice de massa corporal (IMC, circunferência da cintura, razão cintura/estatura e índice de conicidade. A pressão arterial elevada foi caracterizada por valores acima do percentil 90 para pressão arterial sistólica e/ou pressão arterial diastólica. Para identificação dos preditores de pressão arterial elevada, foi adotada a análise das curvas Receiver Operating Characteristic (ROC, com intervalo de confiança de 95%. Posteriormente, identificaram-se os pontos de corte com suas respectivas sensibilidades e especificidades. RESULTADOS: As áreas sob as curvas ROC com os intervalos de confiança foram: rapazes - circunferência de cintura = 0,80 (0,72-0,89; IMC = 0,79 (0,68-0,89; razão cintura/estatura = 0,77 (0,66-0,88; índice de conicidade = 0,69 (0,56-0,81 e para as moças - circunferência de cintura = 0,96 (0,92-1,00; IMC = 0,95 (0,87-1,00; razão cintura/estatura = 0,93 (0,85-1,00; índice de conicidade = 0,74 (0,50-0,98. Os diversos pontos de corte dos indicadores antropométricos com melhores poderes preditivos e suas respectivas sensibilidades e especificidades foram identificados. CONCLUSÃO: Apesar de a razão cintura/estatura e de o IMC terem apresentado boas áreas sob a curva ROC, sugere-se a utilização da circunferência de cintura para a predição da pressão arterial elevada.

  17. Serum IgE levels in patients with intracranial tumors

    Directory of Open Access Journals (Sweden)

    George A Alexiou

    2015-03-01

    Full Text Available Aim: Several epidemiological studies have shown an inverse correlation between allergy and brain cancer. The purpose of this study was to compare the serum IgE levels between patients with gliomas and nonglial tumors and their possible prognostic role. Methods: A total of 84 patients with intracranial tumors were included in this study. At clinical presentation, estimation of serum IgE levels was assessed by nephelometry. Detailed information regarding the history of allergies was collected by interview. Results: Of the 84 cases, 42 were gliomas, 23 were meningiomas, 16 were metastases and 3 were primary central nervous system lymphomas. Patients with high-grade glioma had lower IgE levels than patients with low-grade glioma. Patients with glioma and meningioma had statistical significant lower serum IgE levels than patients with metastases. Patients with glioblastoma with serum IgE levels greater than 24 U/mL had a better survival. Conclusion: Patients with glioma and meningioma had lower IgE levels than patients with metastatic lesions. A prognostic role of serum IgE levels was found in glioblastoma. Further studies in larger patient series are required in order to verify our preliminary observations.

  18. Mechanisms of Anaphylaxis Beyond IgE.

    Science.gov (United States)

    Muñoz-Cano, R; Picado, C; Valero, A; Bartra, J

    2016-01-01

    Anaphylaxis is an acute, life-threatening, multisystem syndrome resulting from the sudden release of mediators derived from mast cells and basophils. Food allergens are the main triggers of anaphylaxis, accounting for 33%-56% of all cases and up to 81% of cases of anaphylaxis in children. Human anaphylaxis is generally thought to be mediated by IgE, with mast cells and basophils as key players, although alternative mechanisms have been proposed. Neutrophils and macrophages have also been implicated in anaphylactic reactions, as have IgG-dependent, complement, and contact system activation. Not all allergic reactions are anaphylactic, and the presence of the so-called accompanying factors (cofactors or augmenting factors) may explain why some conditions lead to anaphylaxis, while in other cases the allergen elicits a milder reaction or is even tolerated. In the presence of these factors, allergic reactions may be induced at lower doses of allergen or become more severe. Cofactors are reported to be relevant in up to 30% of anaphylactic episodes. Nonsteroidal anti-inflammatory drugs and exercise are the best-documented cofactors, although estrogens, angiotensin-converting enzyme inhibitors, β-blockers, lipid-lowering drugs, and alcohol have also been involved. The mechanisms underlying anaphylaxis are complex and involve several interrelated pathways. Some of these pathways may be key to the development of anaphylaxis, while others may only modulate the severity of the reaction. An understanding of predisposing and augmenting factors could lead to the development of new prophylactic and therapeutic approaches.

  19. Triggers of IgE class switching and allergy development

    DEFF Research Database (Denmark)

    Poulsen, Lars K.; Hummelshoj, Lone

    2007-01-01

    type 2 (Th2) T cell subset are the actions of thymic stromal lymphopoietin (TSLP) on dendritic cells and the OX40 ligand on CD4+ T cells. In order for a B lymphocyte to switch to IgE production it needs two signals provided by a Th2 cell in the form of the cytokines interleukin (IL-) 4/IL-13...... the need for more knowledge on preventable causes of IgE- and allergy development....

  20. Occupational asthma and IgE sensitization to grain dust.

    Science.gov (United States)

    Park, H S; Nahm, D H; Suh, C H; Kwon, O Y; Kim, K S; Lee, S W; Chung, H K

    1998-06-01

    To evaluate type I hypersensitivity to grain dust (GD), its prevalence and relationship to respiratory dysfunction, we studied clinical and immunologic features, including skin prick tests (SPT), serum specific IgE, and bronchoprovocation tests of 43 employees working in the animal feed industry. To further characterize IgE-mediated reaction, SDS-PAGE and electroblot studies were performed. Our survey revealed that 15 (34.9%) subjects had work-related skin response (> or =2+ of A/H ratio) to GD, thirteen (30.2%) had high specific IgE antibody against GD. The specific IgE antibody was detected more frequently in symptomatic workers (40%) than in asymptomatic workers (11%). Significant association was found between specific IgE antibody and atopy or smoking (pdust mite, storage mite and corn dust. Immunoblot analysis showed 8 IgE binding components within GD ranging from 13.5 to 142.5 kDa. Two bands (13.5, 33 kDa) were bound to the IgE from more than 50% of the 14 sera tested. In conclusion, these findings suggest that GD inhalation could induce IgE-mediated bronchoconstriction in exposed workers.

  1. Presence of IgE cells in human placenta is independent of malaria infection or chorioamnionitis

    DEFF Research Database (Denmark)

    Rindsjö, E; Hulthén Varli, I; Ofori, M F

    2006-01-01

    We have shown previously that numerous IgE(+) macrophage-like cells are present in the villous stroma of full term placenta and that there was no difference in the amount of IgE(+) cells between allergic and non-allergic mothers. The presence of such an abundant number of IgE(+) cells...... from Ghana with and without malaria parasites. The immunohistochemical staining pattern for IgE looked similar to our previous study, with the IgE located on Hofbauer-like cells. We could not find any difference in the amount or distribution of IgE(+) cells between malaria-infected and non...

  2. Omalizumab decreases IgE production in patients with allergic (IgE-mediated) asthma; PKPD analysis of a biomarker, total IgE

    Science.gov (United States)

    Lowe, Philip J; Renard, Didier

    2011-01-01

    AIM To determine whether excessive IgE production by patients with atopic allergic asthma decreases with omalizumab therapy. METHODS Omalizumab, free and total IgE data were obtained from an epidemiological study and six randomized, double-blind, placebo-controlled trials in patients with allergic asthma. The binding between omalizumab and IgE together with the production and elimination of IgE were modelled as previously, except that, in order to explain why total IgE was decreasing over a period of 5 years, the expression of IgE was allowed to change. RESULTS The prior constant IgE production model failed to converge on the data once long-term observations were included, whereas models allowing IgE production to decrease fitted. A feedback model indicated that, on average, IgE production decreased by 54% per year. This model was further developed with covariate searches indicating clinically small but statistically significant effects of age, gender, body mass index and race on some parameters. Model predictions were checked internally and externally against 3–5 year data from paediatric and adult atopic asthmatic patients and externally against extensive total IgE data from a long-duration (>1 year) phase 1 study which was not used in the model building. CONCLUSIONS A pharmacokinetic–pharmacodynamic model incorporating omalizumab–IgE binding and feedback for control of IgE production indicates that omalizumab reduces production of IgE. This raises the possibility that indefinite treatment may not be required, only for perhaps a few years. After the initial accumulation, total IgE should provide a means to monitor IgE production and guide individual treatment decisions. PMID:21392073

  3. IgE - the main player of food allergy

    DEFF Research Database (Denmark)

    Broekman, Henrike C H; Eiwegger, Thomas; Upton, Julia

    2017-01-01

    Food allergy is a growing problem worldwide, presently affecting 2-4% of adults and 5-8% of young children. IgE is a key player in food allergy. Consequently huge efforts have been made to develop tests to detect either the presence of IgE molecules, their allergen binding sites...... or their functionality, in order to provide information regarding the patient's food allergy. The ultimate goal is to develop tools that are capable of discriminating between asymptomatic sensitization and a clinically relevant food allergy, and between different allergic phenotypes in an accurate and trustworthy manner...

  4. Indicadores antropométricos como preditores de pressão arterial elevada em adolescentes Indicadores antropométricos como predictores de presión arterial elevada en adolescentes Anthropometric indicators as predictors of high blood pressure in adolescents

    Directory of Open Access Journals (Sweden)

    Carmem Cristina Beck

    2011-02-01

    étricos. OBJETIVO: Determinar el poder predictivo de indicadores antropométricos y establecer sus puntos de corte como discriminadores de presión arterial elevada. MÉTODOS: Estudio transversal realizado con una muestra de 660 adolescentes de 14 a 19 años siendo 51,9% jóvenes del sexo femenino. Se tuvieron en cuenta los siguientes indicadores antropométricos: índice de masa corporal (IMC, circunferencia de la cintura, razón cintura/estatura e índice de conicidad. La presión arterial elevada se caracterizó por valores superiores a un percentil 90 para presión arterial sistólica y/o presión arterial diastólica. Para identificación de los predictores de presión arterial elevada, se adoptó el análisis de las curvas Receiver Operating Characteristic (ROC, con intervalo de confianza del 95%. Posteriormente, se identificaron los puntos de corte con sus respectivas sensibilidades y especificidades. RESULTADOS: Las áreas bajo las curvas ROC con los intervalos de confianza fueron: Jóvenes del sexo masculino - circunferencia de cintura = 0,80 (0,72 - 0,89; IMC = 0,79 (0,68 - 0,89; razón cintura/estatura = 0,77 (0,66 - 0,88; índice de conicidad = 0,69 (0,56 - 0,81 y para las jóvenes del sexo femenino - circunferencia de la cintura = 0,96 (0,92 - 1,00; IMC = 0,95 (0,87 - 1,00; razón cintura/estatura = 0,93 (0,85 - 1,00; índice de conicidad = 0,74 (0,50 - 0,98. Se identificaron los diversos puntos de corte de los indicadores antropométricos con mejores poderes predictivos y sus respectivas sensibilidades y especificidades. CONCLUSIÓN: A pesar de la razón cintura/estatura y del IMC haber presentado buenas áreas bajo la curva ROC, se sugiere la utilización de la circunferencia de la cintura para la predicción de la presión arterial elevada.BACKGROUND: Hypertension is related to increased body fat, which can be evaluated by anthropometric indicators. OBJECTIVE: To determine the predictive power of anthropometric indicators and establish their cutoff points as

  5. IgE in lupus pathogenesis: Friends or foes?

    Science.gov (United States)

    Augusto, Jean-François; Truchetet, Marie-Elise; Charles, Nicolas; Blanco, Patrick; Richez, Christophe

    2018-04-01

    Systemic lupus erythematosus (SLE) is a complex autoimmune disease involving multiple immunological pathways. Recently, several studies have suggested an implication of Immunoglobulin E (IgE) in the pathophysiology of SLE. In the Lyn -/- and FcγIIB -/- .Yaa lupus mouse models, autoreactive IgE activate basophils, and promote a Th2 environment with, subsequently, production of autoantibodies by plasma cells. Autoreactive IgE has been also shown to play a role in the activation of human plasmacytoid dendritic cells (pDCs), in synergy with IgG, which results in an increase of interferon-alpha (IFN-α) production. In contrast, a protective effect of total non-autoreactive IgE has also been suggested, through a decreased ability of FcεRI-triggered pDCs to secrete IFN-α. This review summarizes in a comprehensive manner the emerging recent literature in the field, and propose new concepts to reconcile the observations. Copyright © 2018 Elsevier B.V. All rights reserved.

  6. B Cell Intrinsic Mechanisms Constraining IgE Memory

    Directory of Open Access Journals (Sweden)

    Brice Laffleur

    2017-11-01

    Full Text Available Memory B cells and long-lived plasma cells are key elements of adaptive humoral immunity. Regardless of the immunoglobulin class produced, these cells can ensure long-lasting protection but also long-lasting immunopathology, thus requiring tight regulation of their generation and survival. Among all antibody classes, this is especially true for IgE, which stands as the most potent, and can trigger dramatic inflammatory reactions even when present in minute amounts. IgE responses and memory crucially protect against parasites and toxic components of venoms, conferring selective advantages and explaining their conservation in all mammalian species despite a parallel broad spectrum of IgE-mediated immunopathology. Long-term memory of sensitization and anaphylactic responses to allergens constitute the dark side of IgE responses, which can trigger multiple acute or chronic pathologic manifestations, some punctuated with life-threatening events. This Janus face of the IgE response and memory, both necessary and potentially dangerous, thus obviously deserves the most elaborated self-control schemes.

  7. Prevalência e fatores associados à pressão arterial elevada, seu conhecimento e tratamento em idosos no sul do Brasil Prevalencia y factores asociados a la presión arterial elevada, su conocimiento y tratamiento en ancianos en el sur de Brasil Prevalence and factors associated with high blood pressure, awareness, and treatment among elderly in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Maruí Weber Corseuil Giehl

    2013-03-01

    Full Text Available O objetivo foi estimar a prevalência e investigar os fatores associados à pressão arterial elevada, seu conhecimento e tratamento em idosos de Florianópolis, Santa Catarina, Brasil. Trata-se de estudo transversal de base populacional e amostra complexa. A pressão arterial elevada foi definida pela medida do nível pressórico ou uso de anti-hipertensivo, ou diagnóstico prévio. A associação dos desfechos com as variáveis independentes foi verificada pela regressão de Poisson. Foram entrevistados 1.705 idosos. Desses, 84,6% apresentaram pressão arterial elevada, 77,5% estavam cientes da doença e 79,1% a tratavam. A prevalência associou-se à dependência funcional e idade e índice de massa corporal (IMC elevados. Idosos do sexo feminino, idade e IMC elevados, pior percepção em saúde e consulta médica recente estavam mais cientes da doença. O tratamento associou-se a sexo masculino, dependência funcional, pior percepção em saúde e consulta médica recente. Ainda que políticas públicas em saúde devam ser universais, é preciso considerar a desigual distribuição da pressão arterial elevada na população, direcionando-se ações de prevenção, diagnóstico e garantia de tratamento.El objetivo fue estimar la prevalencia e investigar los factores asociados a la presión arterial elevada, su conocimiento y tratamiento en ancianos de Florianópolis, Santa Catarina, Brasil. Se trata de un estudio transversal de base poblacional y muestreo complejo. La presión arterial elevada fue definida por la medida del nivel de presión sanguínea el uso de antihipertensivos, el diagnóstico previo. La asociación de los desenlaces con las variables independientes fue verificada por la regresión de Poisson. Se entrevistaron a 1.705 ancianos. De ellos, un 84,6% presentaron presión arterial elevada, un 77,5% eran conscientes de la enfermedad y un 79,1% la trataban. La prevalencia se asoció a la dependencia funcional y edad e índice de

  8. Comportamiento a tracción a temperaturas ambiente y elevadas de nuevos composites basados en aleaciones hipereutécticas de Al-Si

    Directory of Open Access Journals (Sweden)

    Valer, J.

    1997-02-01

    Full Text Available This work shows the improvement obtained on tensile stress at room and high temperatures of hypereutectic Al-Si alloys. These alloys are produced by a combination of spray-forming, extrusión and thixoforming process, in comparison with conventional casting alloys. Al-25%Si-5%Cu, Al- 25%Si-5%Cu-2%Mg and Al-30%Si-5%Cu alloys have been studied relating their microstructural parameters with tensile stress obtained and comparing them with conventional Al-20%Si, Al-36%Si and Al-50%Si alloys. Al-25%Si-5%Cu alloy was tested before and after semi-solid forming, in order to distinguish the different behaviour of this alloy due to the different microstructure. The properties obtained with these alloys were also related to Al-SiC composites formed by similar processes.

    En este trabajo se muestra la mejora obtenida en la resistencia a la tracción a temperatura ambiente y a elevadas temperaturas de aleaciones hipereutécticas de Al-Si producidas por una combinación de un proceso de solidificación rápida y del conformado en estado semisólido, en comparación con aleaciones obtenidas por procedimientos convencionales de inyección en estado líquido. Se han estudiado las aleaciones Al-25%Si-5%Cu, Al-25%Si-5%Cu-2%Mg y Al-30%Si-5%Cu, relacionando sus parámetros microestructurales con las resistencias a tracción obtenidas, y se han comparado con las aleaciones binarias Al-20%Si, Al-36%Si y Al-50%Si. La aleación Al-25%Si-5%Cu se ha ensayado antes y después del conformado en estado semisólido, lo que ha permitido conocer la diferencia en el comportamiento de la aleación como consecuencia de la distinta microestructura. También se comparan las propiedades obtenidas en estas aleaciones con las que presentan composites de aleaciones de aluminio reforzados con partículas de SiC y procesados por métodos similares.

  9. preescolares desnutridos con madres con obesidad y sin obesidad

    Directory of Open Access Journals (Sweden)

    Viridiana Vanessa Conzuelo-González

    2009-01-01

    Full Text Available El primer objetivo fue conocer cuántos menores de cinco años con diferentes grados de desnutrición tienen una madre con sobrepeso/obesidad/ en una comunidad indígena que vive en extrema pobreza y bajo condiciones de migración masculina internacional. El segundo fue comparar tres variables socionutricionales (ingreso familiar, educación de la madre y adecuación nutrimental de la dieta diaria entre estos hogares y los hogares con desnutrición infantil y madres sin obesidad. Se realizó un estudio transversal (2006-2007, en la comunidad mazahua de San Francisco Tepeolulco, Municipio de Temascalcingo; que incluyó a 85 hogares integrados por preescolares con desnutrición inscritos al programa Oportunidades. Se determinó el estado nutrición de los preescolares con indicadores antropométricos y se obtuvo el IMC de las madres de estos infantes. Se aplicó una encuesta socionutricional, incluida el recordatorio de 24 horas, y complementado con la observación participante (cualitativa. Se encontró que 83% de las madres mazahuas presentaron sobrepeso u obesidad. El estado de nutrición de los preescolares con madres con obesidad presentó un porcentaje mayor de desnutrición (76%. En la variable género, se encontró que 54% de los niños con madres con obesidad tenía baja talla. Al relacionar el nivel educativo de la madre, esta variable resultó ser estadísticamente significativa (p=0.015, donde el analfabetismo está más relacionado con la desnutrición infantil que tienen madres de bajo y/o peso normal. La elevada prevalencia de hogares conformados con preescolares con desnutrición y madres con obesidad, es un síntoma más de la pobreza en zonas indígenas en México, con bajo índice de desarrollo humano.

  10. Total and Candida - Specific IgE in Recurrent Vaginal Candidiasis

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    K V Ratnam

    1987-01-01

    Full Text Available Total and candida specific serum IgE levels were studied in 21 patients who fulfilled the criteria for recurrent vaginal candidiasis, and 45 controls. The candida specific IgE levels were significantly higher in patients with recurrent vaginal candidiasis when compared with the controls. There was no significant difference in the total IgE levels between patients and the controls. IgE is postulated to inhibit the cellular immune respsnse to candida and thereby prevent its eradication. There may be a genetic basis for the increased IgE levels.

  11. Detection of IgE insulin antibody with radioallergosorbent test

    Energy Technology Data Exchange (ETDEWEB)

    Nakagawa, S; Nakayama, H; Sasaki, T; Watanabe, T; Aoki, S [Hokkaido Univ., Sapporo (Japan). 2. Dept. of Medicine; Saito, N [Sapporo Railway Hospital (Japan). Dept. of Medicine

    1978-01-01

    An in vitro method for detecting IgE insulin antibody using the principle of the radioallergosorbent test (RAST) is described. In six patients with insulin allergy, the RAST values were higher than in normal persons or insulin-treated diabetics without insulin allergy. No differences were observed between normal persons and insulin-treated diabetics without insulin allergy. Moreover, it was observed that in one patient treated with highly purified insulin, there was a gradual decrease of RAST value parallel to the radioinsulin binding activity and clinical allergic symptoms. The RAST value of insulin is slightly inhibited by non-IgE antibodies and is, therefore, a semiquantitative value. However, the RAST is simple to perform and reproducible; it is therefore very useful in the detection of IgE insulin antibodies.

  12. Detection of IgE insulin antibody with radioallergosorbent test

    International Nuclear Information System (INIS)

    Nakagawa, S.; Nakayama, H.; Sasaki, T.; Watanabe, T.; Aoki, S.; Saito, N.

    1978-01-01

    An in vitro method for detecting IgE insulin antibody using the principle of the radioallergosorbent test (RAST) is described. In six patients with insulin allergy, the RAST values were higher than in normal persons or insulin-treated diabetics without insulin allergy. No differences were observed between normal persons and insulin-treated diabetics without insulin allergy. Moreover, it was observed that in one patient treated with highly purified insulin, there was a gradual decrease of RAST value parallel to the radioinsulin binding activity and clinical allergic symptoms. The RAST value of insulin is slightly inhibited by non-IgE antibodies and is, therefore, a semiquantitative value. However, the RAST is simple to perform and reproducible; it is therefore very useful in the detection of IgE insulin antibodies. (orig.) [de

  13. Application Protocol, Initial Graphics Exchange Specification (IGES), Layered Electrical Product

    Energy Technology Data Exchange (ETDEWEB)

    O`Connell, L.J. [ed.

    1994-12-01

    An application protocol is an information systems engineering view of a specific product The view represents an agreement on the generic activities needed to design and fabricate the product the agreement on the information needed to support those activities, and the specific constructs of a product data standard for use in transferring some or all of the information required. This application protocol describes the data for electrical and electronic products in terms of a product description standard called the Initial Graphics Exchange Specification (IGES). More specifically, the Layered Electrical Product IGES Application Protocol (AP) specifies the mechanisms for defining and exchanging computer-models and their associated data for those products which have been designed in two dimensional geometry so as to be produced as a series of layers in IGES format The AP defines the appropriateness of the data items for describing the geometry of the various parts of a product (shape and location), the connectivity, and the processing and material characteristics. Excluded is the behavioral requirements which the product was intended to satisfy, except as those requirements have been recorded as design rules or product testing requirements.

  14. Detection of IgE in the sera of rodents: comparison of the applicability of ELISA and RIA

    Energy Technology Data Exchange (ETDEWEB)

    Reiner, G; Zahner, H [Institut fuer Parasitologie der Justus-Liebig-Universitaet Giessen, Germany, F.R.; Haque, A [Institut Pasteur, 59 - Lille (France). Centre d' Immunologie et de Biologie Parasitaire

    1984-04-13

    RIA and ELISA were compared for their ability to detect IgE in different rodent species. With a sheep anti-rat IgE antibody good correlation (P < 0.001) between the 2 assay methods for IgE was found in rats. RIA failed to detect the IgE of Mastomys natalensis while ELISA proved to be a suitable test. However, both tests failed to measure IgE in sera of Nile rats.

  15. Comparative reactivity of human IgE to cynomolgus monkey and human effector cells and effects on IgE effector cell potency

    Science.gov (United States)

    Saul, Louise; Saul, Louise; Josephs, Debra H; Josephs, Debra H; Cutler, Keith; Cutler, Keith; Bradwell, Andrew; Bradwell, Andrew; Karagiannis, Panagiotis; Karagiannis, Panagiotis; Selkirk, Chris; Selkirk, Chris; Gould, Hannah J; Gould, Hannah J; Jones, Paul; Jones, Paul; Spicer, James F; Spicer, James F; Karagiannis, Sophia N; Karagiannis, Sophia N

    2014-01-01

    Background: Due to genetic similarities with humans, primates of the macaque genus such as the cynomolgus monkey are often chosen as models for toxicology studies of antibody therapies. IgE therapeutics in development depend upon engagement with the FcεRI and FcεRII receptors on immune effector cells for their function. Only limited knowledge of the primate IgE immune system is available to inform the choice of models for mechanistic and safety evaluations.   Methods: The recognition of human IgE by peripheral blood lymphocytes from cynomolgus monkey and man was compared. We used effector cells from each species in ex vivo affinity, dose-response, antibody-receptor dissociation and potency assays. Results: We report cross-reactivity of human IgE Fc with cynomolgus monkey cells, and comparable binding kinetics to peripheral blood lymphocytes from both species. In competition and dissociation assays, however, human IgE dissociated faster from cynomolgus monkey compared with human effector cells. Differences in association and dissociation kinetics were reflected in effector cell potency assays of IgE-mediated target cell killing, with higher concentrations of human IgE needed to elicit effector response in the cynomolgus monkey system. Additionally, human IgE binding on immune effector cells yielded significantly different cytokine release profiles in each species. Conclusion: These data suggest that human IgE binds with different characteristics to human and cynomolgus monkey IgE effector cells. This is likely to affect the potency of IgE effector functions in these two species, and so has relevance for the selection of biologically-relevant model systems when designing pre-clinical toxicology and functional studies. PMID:24492303

  16. What are the main environmental exposures associated with elevated IgE in Cuban infants? A population-based study

    Science.gov (United States)

    Fundora-Hernández, Hermes; Venero-Fernández, Silvia J; Suárez-Medina, Ramón; Mora-Faife, Esperanza de la C; García-García, Gladys; del Valle-Infante, Ileana; Gómez-Marrero, Liem; Venn, Andrea; Britton, John; Fogarty, Andrew W

    2014-01-01

    ériques totales chez les nourrissons vivant à La Havane. Méthodes Des échantillons de sang ont été collectés chez 877 nourrissons. Les données sur les symptômes de la maladie allergique et sur une large gamme d'expositions ont été recueillies. Résultats La médiane des IgE était de 35 UI/ml (gamme interquartile: 13 à 96). Le risque d'avoir un taux d'IgE au-dessus de la médiane était plus élevé pour les enfants qui avaient été allaités pendant au moins quatre mois (odds ratio ajusté (OR): 1,28; intervalle de confiance (IC) à 95%: 1,02 à 1,61) et pour les enfants pour qui des cafards ont été signalés dans la maison (OR: 1,30; IC 95%: 1,03 à 1,63). Le risque était plus faible pour les enfants dont la mère possédait un emploi rémunéré (OR: 0,73; IC 95%: 0,54 à 0,97 par rapport à ceux dont les mères n'avaient pas cet emploi, pour les enfants vivant dans des foyers où le gaz et l’électricité étaient utilisés pour la cuisson (OR: 0,45; IC 95%: 0,32 à 0,62 par rapport à l'utilisation de l’électricité seule) et pour les enfants ayant des animaux domestiques à la naissance (OR: 0,83; IC 95%: 0,70 à 1,00). Il n'y avait pas d'association entre les taux d'IgE sériques et l'utilisation de paracétamol. Conclusions Les associations avec la consommation du gaz carburant et l'emploi de la mère indiquent que les taux d'IgE en début de vie sont plus faibles chez les enfants vivant dans une relative richesse. La différence dans l'effet de l'exposition précoce aux animaux de compagnie ou à des cafards pourrait refléter des différences dans ces allergènes, ou être confondu par la richesse relative. Une investigation plus approfondie de cette cohorte permettra de déterminer comment ces effets se traduisent dans l'expression de la maladie allergique plus tard dans la vie. Objetivo La inmunoglobulina E (IgE) juega un papel clave en la patogénesis de la enfermedad alérgica, pero se conoce poco sobre los factores ambientales asociados con

  17. Cross-reactive Carbohydrate Determinant Contributes to the False Positive IgE Antibody to Peanut

    Directory of Open Access Journals (Sweden)

    Komei Ito

    2005-01-01

    Conclusions: Social education about the features of peanut allergy is needed in Japan. Anti-CCD IgE antibody was suggested to be one of the mechanisms contributing to the false positive detection of peanut IgE. Detection of anti-HRP or anti-bromelain IgE can be a useful tool to recognize the presence of anti-CCD antibodies.

  18. Different Implications of Paternal and Maternal Atopy for Perinatal IgE Production and Asthma Development

    OpenAIRE

    Wu, Chih-Chiang; Chen, Rong-Fu; Kuo, Ho-Chang

    2012-01-01

    Asthma is a hereditary disease associated with IgE-mediated reaction. Whether maternal atopy and paternal atopy have different impacts on perinatal IgE production and asthma development remains unclear. This paper reviews and summarizes the effects of maternal and paternal atopy on the developmental aspects of IgE production and asthma. Maternal atopy affects both pre- and postnatal IgE production, whereas paternal atopy mainly affects the latter. Maternally transmitted genes GSTP1 and FceRI-...

  19. IgE to penicillins with different specificities can be identified by a multiepitope macromolecule: Bihaptenic penicillin structures and IgE specificities.

    Science.gov (United States)

    Ariza, A; Barrionuevo, E; Mayorga, C; Montañez, M I; Perez-Inestrosa, E; Ruiz-Sánchez, A; Rodríguez-Guéant, R M; Fernández, T D; Guéant, J L; Torres, M J; Blanca, M

    2014-04-01

    Quantitation of specific IgE by immunoassay is a recommended in vitro test for the diagnosis of immediate hypersensitivity reactions to betalactams (BLs), particularly when skin test results are negative. IgE antibodies that recognize the common nuclear structure of all BLs or the specific side chain structure can be mainly distinguished by immunoassays. The aim of this study was to develop an immunoassay system to detect IgE antibodies with different specificities. Cellulose discs conjugated with benzylpenicillin (BP), amoxicillin (AX) or both drugs, with poly-l-lysine (PLL) as carrier molecule, were used as solid phases in the radioallergosorbent test (RAST). Direct and inhibition radioimmunoassay studies were made to verify the structures recognized by serum IgE antibodies from penicillin-allergic patients. Our results indicated that the addition of both haptens did not decrease the capacity to capture IgE when serum specific to either BP or AX was used, at least in terms of sensitivity. In addition, the inclusion of two haptens improved significantly the levels of IgE detection in patients who recognized both BP and AX. Therefore, the use of a solid phase with a carrier molecule conjugated with two determinants (AX and BP) is helpful to recognize IgE antibodies against either of these determinants and is useful for screening sera with different specificities. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange

    OpenAIRE

    Pennington, Luke F.; Tarchevskaya, Svetlana; Brigger, Daniel; Sathiyamoorthy, Karthik; Graham, Michelle T.; Nadeau, Kari Christine; Eggel, Alexander; Jardetzky, Theodore S.

    2016-01-01

    Omalizumab is a widely used therapeutic anti-IgE antibody. Here we report the crystal structure of the omalizumab-Fab in complex with an IgE-Fc fragment. This structure reveals the mechanism of omalizumab-mediated inhibition of IgE interactions with both high- and low-affinity IgE receptors, and explains why omalizumab selectively binds free IgE. The structure of the complex also provides mechanistic insight into a class of disruptive IgE inhibitors that accelerate the dissociation of the hig...

  1. B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade

    OpenAIRE

    Rebecca K. Martin; Sheela R. Damle; Yolander A. Valentine; Matthew P. Zellner; Briana N. James; Joseph C. Lownik; Andrea J. Luker; Elijah H. Davis; Martha M. DeMeules; Laura M. Khandjian; Fred D. Finkelman; Joseph F. Urban, Jr.; Daniel H. Conrad

    2018-01-01

    Helminth infection is known for generating large amounts of poly-specific IgE. Here we demonstrate that innate-like B1 cells are responsible for this IgE production during infection with the nematode parasites Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. In vitro analysis of B1 cell immunoglobulin class switch recombination to IgE demonstrated a requirement for anti-CD40 and IL-4 that was further enhanced when IL-5 was added or when the B1 source was helminth infected mi...

  2. Mortalidad intrahospitalaria en pacientes con diarrea asociada a infección por Clostridium difficile

    Directory of Open Access Journals (Sweden)

    A. Ramírez-Rosales

    2012-04-01

    Conclusión: Encontramos una mortalidad elevada en pacientes con DACD hospitalizados en la Unidad de Cuidados Intensivos, lo cual nos obliga a mantener una vigilancia estrecha en esta población, para realizar una detección y tratamiento oportuno.

  3. Respuesta IgE específica anti-Blomia tropicalis en niños asmáticos residentes en Santa Marta, una ciudad del Caribe colombiano

    Directory of Open Access Journals (Sweden)

    Dary Luz Mendoza Meza

    2013-10-01

    Full Text Available ResumenBlomia tropicalis es un ácaro intradomiciliario predominante en Colombia. Sin embargo, existe poca información disponible sobre la sensibilización a este y otros ácaros intradomiciliarios en pacientes asmáticos de Santa Marta. El propósito de este estudio fue establecer la respuesta IgE específica contra el ácaro B. tropicalis en pacientes pediátricos asmáticos de Santa Marta. La respuesta IgE específica se determinó por ELISA indirecto en 77 niños con diagnóstico de asma bronquial alérgica. Las fracciones alergénicas mayoritarias de B. tropicalis se identificaron por Western Blotting usando un extracto de proteínas de B. tropicalis. Setenta y seis (98.7% niños presentaron niveles elevados de IgE total, 48 (88% tenían IgE anti- B. tropicalis positiva. El Western Blot identificó 16 fracciones alergénicas con rango entre 80- 21 kDa. Estos resultados indican que los alérgenos del ácaro B. tropicalis deben incluirse en el diagnóstico exacto de la sensibilización a los ácaros del polvo doméstico en Santa Marta, Colombia. (DUAZARY 2011, 9 - 16AbstractBlomia tropicalis is the predominant indoor mite in Colombia, nevertheless there is not enough information about sensitization to this and other indoor mites, in the asthmatic patients living in Santa Marta. The purpose of this study was to establish the specific IgE responses against to B. tropicalis in pediatric asthmatic patients in Santa Marta. Specific IgE responses were determined by indirect ELISA in 77 children with bronchial asthma. The B. tropicalis major allergenic fractions were identified by Western Blotting using a B. tropicalis protein extract. Seventy six (98.7% children showed high levels of total IgE, 48 (88% had IgE anti-B. tropicalis positive. Sixteen allergenic fractions, between 80 – 21 kDa, were identified by Western Blotting. These results indicate that the B. tropicalisallergens must be included in the precise diagnosis of sensitization to house

  4. Diagnostic Utility of Total IgE in Foods, Inhalant, and Multiple Allergies in Saudi Arabia.

    Science.gov (United States)

    Al-Mughales, Jamil A

    2016-01-01

    Objective. To assess the diagnostic significance of total IgE in foods, inhalant, and multiple allergies. Methods. Retrospective review of the laboratory records of patients who presented with clinical suspicion of food or inhalant allergy between January 2013 and December 2014. Total IgE level was defined as positive for a value >195 kU/L; and diagnosis was confirmed by the detection of specific IgE (golden standard) for at least one food or inhalant allergen and at least two allergens in multiple allergies. Results. A total of 1893 (male ratio = 0.68, mean age = 39.0 ± 19.2 years) patients were included. Total IgE had comparable sensitivity (55.8% versus 59.6%) and specificity (83.9% versus 84.4%) in food versus inhalant allergy, respectively, but a superior PPV in inhalant allergy (79.1% versus 54.4%). ROC curve analysis showed a better diagnostic value in inhalant allergies (AUC = 0.817 (95% CI = 0.796-0.837) versus 0.770 (95% CI = 0.707-0.833)). In multiple allergies, total IgE had a relatively good sensitivity (78.6%), while negative IgE testing (allergies with 91.5% certitude. Conclusion. Total IgE assay is not efficient as a diagnostic test for foods, inhalant, or multiple allergies. The best strategy should refer to specific IgE testing guided by a comprehensive atopic history.

  5. Control of IgE and IgGl antibody production in mice

    International Nuclear Information System (INIS)

    De Macedo, M.S.; Braga, F.; Mota, I.

    1976-01-01

    The production of IgE and IgCl was studied in untreated, thymectomized, splenectomized, anti-thymocyte serum-treated, or sublethally X-irradiated mice. Dinitrophenyl, Ascaris, and ovalbumin were used as antigens, and aluminum hydroxide was used as adjuvant. A suppression of IgE production was observed in adult thymectomized mice, although the kinetic pattern of the antibody response was the same as in control animals. IgGl antibody production was not affected by thymectomy. Splenectomy did not change either IgE or IgGl production. A single dose of rabbit antithymocyte serum (ATS) given 8 days after immunization inhibited IgE antibody production. The effect of ATS was dose dependent and also varied with the amount of antigen used, the immune response to high doses being more susceptible to the effect of ATS. No alteration in IgGl production was caused by ATS even when IgE antibody formation was completely inhibited. When preceding immunization, sublethal irradiation enhanced IgE antibody formation and partially suppressed IgGl production; applied after immunization, irradiation caused an enhancement of IgE production which was inversely proportional to the interval elapsed between the two procedures. On the other hand, the IgGl antibody production was fairly resistant to the same treatment. The results suggest a clearcut separation between the mechanisms regulating IgE and IgGl production in mice

  6. Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange

    Science.gov (United States)

    Pennington, Luke F.; Tarchevskaya, Svetlana; Brigger, Daniel; Sathiyamoorthy, Karthik; Graham, Michelle T.; Nadeau, Kari Christine; Eggel, Alexander; Jardetzky, Theodore S.

    2016-01-01

    Omalizumab is a widely used therapeutic anti-IgE antibody. Here we report the crystal structure of the omalizumab–Fab in complex with an IgE-Fc fragment. This structure reveals the mechanism of omalizumab-mediated inhibition of IgE interactions with both high- and low-affinity IgE receptors, and explains why omalizumab selectively binds free IgE. The structure of the complex also provides mechanistic insight into a class of disruptive IgE inhibitors that accelerate the dissociation of the high-affinity IgE receptor from IgE. We use this structural data to generate a mutant IgE-Fc fragment that is resistant to omalizumab binding. Treatment with this omalizumab-resistant IgE-Fc fragment, in combination with omalizumab, promotes the exchange of cell-bound full-length IgE with omalizumab-resistant IgE-Fc fragments on human basophils. This combination treatment also blocks basophil activation more efficiently than either agent alone, providing a novel approach to probe regulatory mechanisms underlying IgE hypersensitivity with implications for therapeutic interventions. PMID:27194387

  7. Serum vitamin D and IgE levels in infants and children under 2 years ...

    African Journals Online (AJOL)

    Ehab

    Serum vitamin D and IgE levels in infants and children under 2 years of age with recurrent chest ... from 6-24 months, diagnosed to have recurrent wheeze (>3 attacks), recruited ... IgE and the development of allergic sensitization29. Hence this ...

  8. B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade

    Directory of Open Access Journals (Sweden)

    Rebecca K. Martin

    2018-02-01

    Full Text Available Helminth infection is known for generating large amounts of poly-specific IgE. Here we demonstrate that innate-like B1 cells are responsible for this IgE production during infection with the nematode parasites Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. In vitro analysis of B1 cell immunoglobulin class switch recombination to IgE demonstrated a requirement for anti-CD40 and IL-4 that was further enhanced when IL-5 was added or when the B1 source was helminth infected mice. An IL-25-induced upregulation of IgE in B1 cells was also demonstrated. In T cell-reconstituted RAG1−/− mice, N. brasiliensis clearance was enhanced with the addition of B2 cells in an IgE-dependent manner. This enhanced clearance was impeded by reconstitution with IgE sufficient B1 cells. Mucosal mast cells mediated the B2 cell enhancement of clearance in the absence of B1 cells. The data support B1 cell IgE secretion as a regulatory response exploited by the helminth.

  9. Resultados a corto-medio plazo en revascularización miocárdica aislada con injerto de arteria mamaria interna bilateral en 497 pacientes

    Directory of Open Access Journals (Sweden)

    Carlos-Esteban Martín López

    2016-01-01

    Conclusiones: La cirugía de revascularización miocárdica con doble mamaria presenta unos excelentes resultados a corto y medio plazo independientemente de la edad. En pacientes con elevada comorbilidad, especialmente vasculopatía e insuficiencia renal, se debería individualizar la decisión del uso de doble mamaria.

  10. Total and antigen-specific Ige levels in umbilical cord blood

    Directory of Open Access Journals (Sweden)

    Sybilski AJ

    2009-12-01

    Full Text Available Abstract The present study was conducted to learn whether the perinatal and environmental factors could influence the total and antigen-specific IgE levels in umbilical cord blood. Retrospective data were obtained from 173 mother-infant pairs. Total and specific (for children's food, wheat/grass and house dust mite-HDM cord blood IgE levels were determined using the immunoassay test. The total cord blood IgE was between 0.0-23.08 IU/ml (mean 0.55 ± 2.07 IU/ml; median 0.16 IU/ml. Total IgE levels were significantly higher in boys compared with girls (OR = 2.2; P = 0.007, and in newborns with complicated pregnancy (OR = 2.7; P = 0.003. A greater number of siblings correlated with increases in the total cord blood IgE (P

  11. Dynamics of plasma levels of specific IgE in chlorhexidine allergic patients with and without accidental re-exposure

    DEFF Research Database (Denmark)

    Opstrup, Morten Schjørring; Poulsen, Lars K.; Malling, Hans Jørgen

    2016-01-01

    longer time periods is lacking and it is unknown whether levels fall below influences levels of specific IgE. OBJECTIVE: To investigate the dynamics of specific IgE in chlorhexidine allergic patients...

  12. Repeated vaccination with tetanus toxoid of plasma donors with pre-existing specific IgE transiently elevates tetanus-specific IgE but does not induce allergic symptoms

    NARCIS (Netherlands)

    Unger, Peter-Paul A.; Makuch, Mateusz; Aalbers, Marja; Derksen, Ninotska I. L.; ten Brinke, Anja; Aalberse, Rob C.; Rispens, Theo; van Ham, S. Marieke

    2018-01-01

    IgE responses against allergens have acquired much attention due to their pathogenic nature as mediators of allergic reactions. In contrast, IgE responses against vaccines like Diphtheria-Tetanus-Pertussis (DTP) and the potential persistence of IgE production have received relatively little

  13. Síndrome de Waardenburg. Presentación de un caso con glaucoma pigmentario

    Directory of Open Access Journals (Sweden)

    Sol Inés Parapar Tena

    2017-11-01

    Caso clínico: Paciente femenina de 30 años de edad que acude con diagnóstico de síndrome de Waardenburg tipo ii subtipo B, con heterocromía del iris, huso de Krukenberg, hipermetropía, hiperpigmentación de la malla trabecular del ángulo iridocorneal y presión ocular ligeramente elevada que nos hicieron pensar en un glaucoma pigmentario.

  14. Measurement of IgE antibodies against purified grass pollen allergens (Lol p 1, 2, 3 and 5) during immunotherapy.

    Science.gov (United States)

    Van Ree, R; Van Leeuwen, W A; Dieges, P H; Van Wijk, R G; De Jong, N; Brewczyski, P Z; Kroon, A M; Schilte, P P; Tan, K Y; Simon-Licht, I F; Roberts, A M; Stapel, S O; Aalberse, R C

    1997-01-01

    IgE titres tend to rise early after the start of immunotherapy, followed by a decline to pre-immunotherapy levels or lower. We were interested to know whether the early increase in IgE antibodies includes new specificities of IgE, and whether these responses persist. Sera of 64 patients undergoing grass pollen immunotherapy were tested for IgE against four purified grass pollen allergens: Lol p 1, 2, 3, and 5. At least two serum samples were taken, one before the start of therapy and one between 5 and 18 months after the first immunization (mean: 10 months). The mean IgE responses to Lol p 1, 2 and 3 showed a moderate but not significant increase. In contrast, the mean IgE response to Lol p 5 showed a significant decrease of > 30%. IgE against total Lohum perenne pollen extract moderately increased (> 20%), showing that a RAST for total pollen is not always indicative for the development of IgE against its major allergens. For > 40% of the patients it was found that IgE against one or more of the four allergens increased, while IgE against the remaining allergen(s) decreased. For 10 sera the ratio of IgE titres against at least two allergens changed by at least a factor of 5. The changes in specific IgE also included conversions from negative (< 0.1 RU) to positive (0.6 to 5.0 RU) for five patients. For two patients, the induction of these 'new' IgE antibodies against major allergens was shown to result in a response that was persistent over several years. Although active induction of new IgE specificities by immunotherapy was not really proven, the observations in this study indicate that monitoring of IgE against purified (major) allergens is necessary to evaluate changes in specific IgE in a reliable way.

  15. Nanoparticles rapidly assess specific IgE in plasma

    International Nuclear Information System (INIS)

    Ashraf, Sarmadia; Qadri, Shahnaz; Al-Ramadi, Basel; Haik, Yousef

    2012-01-01

    Allergy is the sixth leading cause of chronic disease in the world. This study demonstrates the feasibility of detecting allergy indicators in human plasma, noninvasively, at the point of care and with a comparable efficiency and reduced turnaround time compared with the gold standard. Peanut allergy was utilized as a model due to its widespread occurrence among the US population and fatality if not treated. The detection procedure utilized magnetic nanoparticles that were coated with an allergen layer (peanut protein extract). Peanut immunoglobulin E (IgE) was detected in concentrations close to the minimum detection range of CAP assay. The results were obtained in minutes compared with the CAP assay which requires more than 3 h. (paper)

  16. Measurement of IgE antibodies against purified grass pollen allergens (Lol p 1, 2, 3 and 5) during immunotherapy

    NARCIS (Netherlands)

    van Ree, R.; van Leeuwen, W. A.; Dieges, P. H.; van Wijk, R. G.; de Jong, N.; Brewczyski, P. Z.; Kroon, A. M.; Schilte, P. P.; Tan, K. Y.; Simon-Licht, I. F.; Roberts, A. M.; Stapel, S. O.; Aalberse, R. C.

    1997-01-01

    BACKGROUND: IgE titres tend to rise early after the start of immunotherapy, followed by a decline to pre-immunotherapy levels or lower. OBJECTIVES: We were interested to know whether the early increase in IgE antibodies includes new specificities of IgE, and whether these responses persist. METHODS:

  17. Staphylococcal enterotoxin-specific IgE antibodies in atopic dermatitis.

    Science.gov (United States)

    Ide, Fumihito; Matsubara, Tomoyo; Kaneko, Miho; Ichiyama, Takashi; Mukouyama, Tokuko; Furukawa, Susumu

    2004-06-01

    The authors clarified the clinical significance of the measurement of serum concentrations of specific IgE antibodies to staphylococcal enterotoxin (SE) A- and SEB in atopic dermatitis (AD). The serum concentrations of SEA- and SEB-specific IgE antibodies in 140 pediatric patients with AD were measured with an immuno CAP -radioallergosorbent test system (RAST). To check the cross-reaction of specific IgE antibodies to SEA/SEB and other allergens, the CAP RAST fluorescent enzyme immunoassay inhibition test was performed. Forty-seven patients (33.6%) tested positive for either SEA- or SEB-specific IgE antibodies. School children showed higher positive rates of SEA/SEB-specific IgE antibodies than infants or young children. The patients with severe AD and those with exacerbation of symptoms in summer, had higher positive rates of SEA/SEB-specific IgE antibodies than patients with mild AD or those with exacerbation in winter. In addition, the positive rates of specific IgE antibodies to both dog-dander and cat-dander were higher in patients with positive SEA/SEB-specific IgE antibodies than in patients with negative ones. No cross-reactions occurred among specific IgE antibodies to SEA/SEB and dog/cat dander with one patient's serum, which had positive IgE-specific antibodies against cat/dog dander and SEA/SEB. The positive rate of SEA/SEB-specific IgE antibodies in the patients with dogs and/or cats as pets was 48.4%, which was higher than in those with no pets. Atopic dermatitis patients who exhibit high positive rates of SEA/SEB-specific IgE antibodies were found to be school children, severe cases, cases with high serum concentrations of total IgE, cases with exacerbation in summer, and cases with dogs and/or cats as pets. The measurement of serum concentrations of specific IgE antibodies to SEA and SEB, thus has some value for evaluating AD patients.

  18. Two Loci on Chromosome 5 Are Associated with Serum IgE Levels in Labrador Retrievers

    Science.gov (United States)

    Owczarek-Lipska, Marta; Lauber, Béatrice; Molitor, Vivianne; Meury, Sabrina; Kierczak, Marcin; Tengvall, Katarina; Webster, Matthew T.; Jagannathan, Vidhya; Schlotter, Yvette; Willemse, Ton; Hendricks, Anke; Bergvall, Kerstin; Hedhammar, Åke; Andersson, Göran; Lindblad-Toh, Kerstin; Favrot, Claude; Roosje, Petra; Marti, Eliane; Leeb, Tosso

    2012-01-01

    Crosslinking of immunoglobulin E antibodies (IgE) bound at the surface of mast cells and subsequent mediator release is considered the most important trigger for allergic reactions. Therefore, the genetic control of IgE levels is studied in the context of allergic diseases, such as asthma, atopic rhinitis, or atopic dermatitis (AD). We performed genome-wide association studies in 161 Labrador Retrievers with regard to total and allergen-specific immunoglobulin E (IgE) levels. We identified a genome-wide significant association on CFA 5 with the antigen-specific IgE responsiveness to Acarus siro. We detected a second genome-wide significant association with respect to the antigen-specific IgE responsiveness to Tyrophagus putrescentiae at a different locus on chromosome 5. A. siro and T. putrescentiae both belong to the family Acaridae and represent so-called storage or forage mites. These forage mites are discussed as major allergen sources in canine AD. No obvious candidate gene for the regulation of IgE levels is located under the two association signals. Therefore our studies offer a chance of identifying a novel mechanism controlling the host's IgE response. PMID:22720065

  19. Mouse Chromosome 4 Is Associated with the Baseline and Allergic IgE Phenotypes

    Directory of Open Access Journals (Sweden)

    Cynthia Kanagaratham

    2017-08-01

    Full Text Available Regulation of IgE concentration in the blood is a complex trait, with high concentrations associated with parasitic infections as well as allergic diseases. A/J strain mice have significantly higher plasma concentrations of IgE, both at baseline and after ovalbumin antigen exposure, when compared to C57BL/6J strain mice. Our objective was to determine the genomic regions associated with this difference in phenotype. To achieve this, we used a panel of recombinant congenic strains (RCS derived from A/J and C57BL/6J strains. We measured IgE in the RCS panel at baseline and following allergen exposure. Using marker by marker analysis of the RCS genotype and phenotype data, we identified multiple regions associated with the IgE phenotype. A single region was identified to be associated with baseline IgE level, while multiple regions wereassociated with the phenotype after allergen exposure. The most significant region was found on Chromosome 4, from 81.46 to 86.17 Mbp. Chromosome 4 substitution strain mice had significantly higher concentration of IgE than their background parental strain mice, C57BL/6J. Our data presents multiple candidate regions associated with plasma IgE concentration at baseline and following allergen exposure, with the most significant one located on Chromosome 4.

  20. Aeroallergen and food IgE sensitization and local and systemic inflammation in asthma.

    Science.gov (United States)

    Patelis, A; Janson, C; Borres, M P; Nordvall, L; Alving, K; Malinovschi, A

    2014-03-01

    We recently reported an independent association between IgE sensitization to food allergens and increased airway inflammation, assessed by fraction of exhaled nitric oxide (FeNO), in a population-based study (J Allergy Clin Immunol, 130, 2012, 397). Similar studies have not been performed in populations with asthma. The aim of the present study was to investigate the allergic sensitization profile in asthmatics and examine FeNO, airway responsiveness and blood eosinophilia in relation to type and degree of IgE sensitization. FeNO, airway responsiveness, blood eosinophil count (B-Eos) and IgE sensitization to food allergens and aeroallergens were determined in 408 subjects with asthma, aged 10-34 years. Asthmatics had higher prevalence of IgE sensitization against all allergens than controls (P < 0.001). Mite, pollen, furry animal, mould and food sensitizations were each associated with increased FeNO, airway responsiveness and B-Eos in asthmatics. IgE sensitization to mould, furry animals and food allergens was independently related to FeNO (all P < 0.05) after adjustment for age, sex, height, smoking history and medication. IgE sensitization to mould (P < 0.001) and furry animals (P = 0.02) was related to airway responsiveness in a similar model. Finally, IgE sensitization to mould (P = 0.001), furry animals (P < 0.001) and food allergens (P < 0.001) was independently related to B-Eos. Independent effects of IgE sensitization to aeroallergens (furry animals and mould) and food allergens were found on both local and systemic markers of inflammation in asthma. The finding regarding food IgE sensitization is novel, and a clinical implication might be that even food sensitization must be assessed to fully understand inflammation patterns in asthma. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  1. EFFICACY OF COMBINATION TREATMENT WITH ANTI_IGE PLUS SPECIFIC IMMUNOTHERAPY IN PATIENTS WITH ATOPIC DISEASES

    Directory of Open Access Journals (Sweden)

    N.I. Il'ina

    2008-01-01

    Full Text Available Allergen specific immunotherapy (ASIT is a very effective technique in treatment of many allergic diseases. It greatly improves the quality of life. There's a risk of adverse system reactions at the time of ASIT. Treatment with anti Ige antibodies (omalizumab, xolair allows decreasing the circulating Ige level and lessening an expression of high affinity fc_r1 receptors on the surface of basophiles and mast cells, inhibition of early and late phase of allergic inflammatory response. Combination of antibige therapy and ASIT can lead to decrease of risk of adverse system reactions.Key words: omalizumab, anti Ige antibodies, allergen specific immunotherapy.

  2. Novel IgE Inhibitors for the Treatment of Food Allergies

    Science.gov (United States)

    2016-10-01

    our chances of identifying inhibitors of the IgE:FcR Figure 4. Yeast surface display and selection of mutated anti-IgE scFv clone 18 from an error...complex was used to generate a point mutation in the IgE-Fc, yielding an omalizumab-resistant IgE. Omalizumab-resistant IgE, in combination with...display experiments with wt and mutant IgE-Fc Month 10 Obtained yeast display library, generated selection reagents (IgE), completed anti-IgE

  3. Contribution of Phadebas IgE test to typification of allergic patients

    International Nuclear Information System (INIS)

    Cap, J.; Spanar, E.; Holan, J.; Zahradny, V.

    1976-01-01

    Experience is reported with the examination of serum immunoglobulin E in a control group (n=22), in a group of asthmatics with bacterial allergy (n=25) and in a group of asthmatics with antibody allergy (n=33). In the examination by radioimmunosorbent method (Phadebas IgE Test) the levels of IgE over 500 mU/ml were considered as proof of allergy of the antibody type where other etiological factors could be excluded. A marked immunosuppressive effect of corticoids on the level of IgE is pointed out. The method mentioned is considered as suitable for the objective typification of allergic patients. (author)

  4. Radioimmunoassay of total IgE and allergen-specific IgE antibodies with a uniform indicator system in allergies of childhood

    International Nuclear Information System (INIS)

    Struy, H.; Schuster, R.; Sollich, V.; Thal, W.; Morenz, J.

    1984-01-01

    Solid-phase radioimmunoassays for the determination of allergen-specific and total IgE have been developed. In an indirect solid-phase radioimmunoassay for the measurement of allergen-specific antibodies PVC blisters coated with allergens and in a sandwich solid-phase radioimmunoassay blisters coated with antihuman IgE antibodies are incubated sequentially with patient serum, unlabelled antihuman IgE from rabbits purified by affinity chromatography, and finally with antirabbitglobulin from sheep. Antirabbitglobuline was purified by immunoadsorption. The 125 I-labelled antibody with a specific activity of 30 kBq/μg antibody protein could be used universally for the determination of antibodies of each immunoglobulin class. In 160 patients mostly with seasonal asthma these assays supported RAST and PRIST kits and were helpful in the diagnosis of atopic diseases. (author)

  5. Registro Español de Ablación con Catéter. III Informe Oficial (2003

    Directory of Open Access Journals (Sweden)

    Enrique Rodríguez Font

    2005-04-01

    La elevada participación y la consistencia de los resultados con los de años previos ayudan a consolidar este registro como referencia para el resto de la comunidad científica. Los resultados del registro prospectivo ofrecen una información de mejor calidad y más detallada en sus resultados y complicaciones.

  6. Serum levels of total IgE and soluble CD23 in bronchial asthma

    Directory of Open Access Journals (Sweden)

    G. Di Lorenzo

    1996-01-01

    Full Text Available The aim of the present study was to compare, during the pollen season, serum levels of total IgE and soluble CD23 (sCD23 from patients with allergic bronchial asthma, with those from healthy subjects. Significantly higher levels of total IgE and sCD23 were found in patients with asthma compared to the control group. Both in normal controls and in asthmatic patients, a significant correlation was shown between the levels of these two molecules. In asthmatic patients, significant correlations were found for both total IgE and sCD23, with lung function measured as bronchial responsiveness to inhaled methacholine. These results suggest that in asthmatic patients, in addition to the study of total serum IgE levels, the assessment of sCD23 serum levels may be helpful in the evaluation of disease activity.

  7. Different implications of paternal and maternal atopy for perinatal IgE production and asthma development.

    Science.gov (United States)

    Wu, Chih-Chiang; Chen, Rong-Fu; Kuo, Ho-Chang

    2012-01-01

    Asthma is a hereditary disease associated with IgE-mediated reaction. Whether maternal atopy and paternal atopy have different impacts on perinatal IgE production and asthma development remains unclear. This paper reviews and summarizes the effects of maternal and paternal atopy on the developmental aspects of IgE production and asthma. Maternal atopy affects both pre- and postnatal IgE production, whereas paternal atopy mainly affects the latter. Maternally transmitted genes GSTP1 and FceRI-beta are associated with lung function and allergic sensitization, respectively. In IgE production and asthma development, the maternal influence on gene-environment interaction is greater than paternal influence. Maternal, paternal, and/or postnatal environmental modulation of allergic responses have been linked to epigenetic mechanisms, which may be good targets for early prevention of asthma.

  8. Different Implications of Paternal and Maternal Atopy for Perinatal IgE Production and Asthma Development

    Directory of Open Access Journals (Sweden)

    Chih-Chiang Wu

    2012-01-01

    Full Text Available Asthma is a hereditary disease associated with IgE-mediated reaction. Whether maternal atopy and paternal atopy have different impacts on perinatal IgE production and asthma development remains unclear. This paper reviews and summarizes the effects of maternal and paternal atopy on the developmental aspects of IgE production and asthma. Maternal atopy affects both pre- and postnatal IgE production, whereas paternal atopy mainly affects the latter. Maternally transmitted genes GSTP1 and FceRI-beta are associated with lung function and allergic sensitization, respectively. In IgE production and asthma development, the maternal influence on gene-environment interaction is greater than paternal influence. Maternal, paternal, and/or postnatal environmental modulation of allergic responses have been linked to epigenetic mechanisms, which may be good targets for early prevention of asthma.

  9. The who, where, and when of IgE in allergic airway disease.

    Science.gov (United States)

    Dullaers, Melissa; De Bruyne, Ruth; Ramadani, Faruk; Gould, Hannah J; Gevaert, Philippe; Lambrecht, Bart N

    2012-03-01

    Allergic asthma and allergic rhinitis/conjunctivitis are characterized by a T(H)2-dominated immune response associated with increased serum IgE levels in response to inhaled allergens. Because IgE is a key player in the induction and maintenance of allergic inflammation, it represents a prime target for therapeutic intervention. However, our understanding of IgE biology remains fragmentary. This article puts together our current knowledge on IgE in allergic airway diseases with a special focus on the identity of IgE-secreting cells ("who"), their location ("where"), and the circumstances in which they are induced ("when"). We further consider the therapeutic implications of the insights gained. Copyright © 2011 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

  10. Update on Hyper IgE syndrome (HIES

    Directory of Open Access Journals (Sweden)

    Javad Ghaffari

    2012-02-01

    Full Text Available Hyper IgE Syndrome (HIES is a rare primary immunodeficiency disease. Most of HIES cases are sporadic. Autosomal dominant HIES is caused by mutation in signal transducer and activator of transcription-3 (STAT-3. A number of mosaicism HIES has been reported that is associated with intermediate phenotype. Autosomal recessive HIES is due to mutation in Dock-8 or cytokine sis 8 and TYK2 or tyrosine kinase 2. The common anifestations are atopic eczema, staphylococcal dermatitis, cellulitis and folliculitis (cold dermal abscesses that are not warm, painful and without redness, recurrent pneumonia and pulmonary abscesses, osteopenia and recurrent bone fracture. The diagnosis of standard HIES is based on clinical suspicion. There is no specific treatment for HIES. The treatment should be based on the prevention of developing infections. Prophylactic antibiotics such as cotrimoxazole and IVIG are administered. Hematopoietic stem cell transplantation was done for all types of HIES, but there is a little information and experience about the long term results of this therapy.

  11. An IgE epitope of Bet v 1 and fagales PR10 proteins as defined by a human monoclonal IgE

    DEFF Research Database (Denmark)

    Hecker, J.; Diethers, A.; Schulz, D.

    2012-01-01

    -reactivities predicted by primary structure analyses of different isoforms and PR10 proteins were verified by allergen chip-based analyses. CONCLUSIONS: The obtained results demonstrate that hybrid IgE repertoires represent a source for human antibodies with genuine paratopes. The IgE-derived information about the Ig...... generation and epitope delineation of a human monoclonal IgE against the prototypic allergen Bet v 1. METHODS: Phage-display scFv hybrid libraries of allergic donor-derived VH epsilon and synthetic VL were established from 107 mononuclear cells. An obtained scFv was converted into human immunoglobulin...

  12. Microarray-based IgE detection in tears of patients with vernal keratoconjunctivitis.

    Science.gov (United States)

    Leonardi, Andrea; Borghesan, Franco; Faggian, Diego; Plebani, Mario

    2015-11-01

    A specific allergen sensitization can be demonstrated in approximately half of the vernal keratoconjunctivitis (VKC) patients by conventional allergic tests. The measurement of specific IgE in tears using a multiplex allergen microarray may offer advantages to identify local sensitization to a specific allergen. In spring-summer 2011, serum and tears samples were collected from 10 active VKC patients (three females, seven males) and 10 age-matched normal subjects. Skin prick test, symptoms score and full ophthalmological examination were performed. Specific serum and tear IgE were assayed using ImmunoCAP ISAC, a microarray containing 103 components derived from 47 allergens. Normal subjects resulted negative for the presence of specific IgE both in serum and in tears. Of the 10 VKC patients, six resulted positive to specific IgE in serum and/or tears. In three of these six patients, specific IgE was found positive only in tears. Cross-reactivity between specific markers was found in three patients. Grass, tree, mites, animal but also food allergen-specific IgE were found in tears. Conjunctival provocation test performed out of season confirmed the specific local conjunctival reactivity. Multiple specific IgE measurements with single protein allergens using a microarray technique in tear samples are a useful, simple and non-invasive diagnostic tool. ImmunoCAP ISAC detects allergen sensitization at component level and adds important information by defining both cross- and co-sensitization to a large variety of allergen molecules. The presence of specific IgE only in tears of VKC patients reinforces the concept of possible local sensitization. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  13. Serum IgE reactivity profiling in an asthma affected cohort.

    Directory of Open Access Journals (Sweden)

    Tania Dottorini

    Full Text Available BACKGROUND: Epidemiological evidence indicates that atopic asthma correlates with high serum IgE levels though the contribution of allergen specific IgE to the pathogenesis and the severity of the disease is still unclear. METHODS: We developed a microarray immunoassay containing 103 allergens to study the IgE reactivity profiles of 485 asthmatic and 342 non-asthmatic individuals belonging to families whose members have a documented history of asthma and atopy. We employed k-means clustering, to investigate whether a particular IgE reactivity profile correlated with asthma and other atopic conditions such as rhinitis, conjunctivitis and eczema. RESULTS: Both case-control and parent-to-siblings analyses demonstrated that while the presence of specific IgE against individual allergens correlated poorly with pathological conditions, particular reactivity profiles were significantly associated with asthma (p<10E-09. An artificial neural network (ANN-based algorithm, calibrated with the profile reactivity data, correctly classified as asthmatic or non-asthmatic 78% of the individual examined. Multivariate statistical analysis demonstrated that the familiar relationships of the study population did not affect the observed correlations. CONCLUSIONS: These findings indicate that asthma is a higher-order phenomenon related to patterns of IgE reactivity rather than to single antibody reactions. This notion sheds new light on the pathogenesis of the disease and can be readily employed to distinguish asthmatic and non-asthmatic individuals on the basis of their serum reactivity profile.

  14. Evaluation of FcεRl-binding serum IgE in patients with ocular allergic diseases

    Directory of Open Access Journals (Sweden)

    Satoru Matsumoto

    1999-01-01

    Full Text Available We evaluated high-affinity receptor for IgE (FcεRI- binding serum IgE in patients with atopic keratoconjunctivitis (AKC; n=31 and with seasonal allergic conjunctivitis (SAC; n=13 by enzyme-linked immunosorbent assay (ELISA using a recombinant soluble form of the human FcεRIα ectodomain (soluble α. The quantities of FcεRI-binding IgE are compared with those of total IgE measured by a conventional sandwich ELISA. Both of the quantities of FcεRI-binding and total IgE in AKC were significantly larger than those in SAC (P<0.001. In contrast, the proportion of FcεRI- binding IgE (FcεRI-binding IgE/total IgE; % in SAC was significantly larger than that in AKC (P <0.001, although significant reverse correlation was observed between the proportion of FcεRI-binding IgE and total IgE in both AKC and SAC. Significantly, a higher proportion of FcεRI-binding IgE in SAC than that in AKC may reflect the differences in pathologic states of AKC and SAC that are caused by a disparity in immune responses in these diseases.

  15. Elevada densidade de semeadura aumenta a produtividade da soja? Respostas da radiação fotossinteticamente ativa

    Directory of Open Access Journals (Sweden)

    Fabiano André Petter

    2016-01-01

    Full Text Available Resumo Estudos recentes têm abordado o uso da prática da elevada densidade de semeadura na cultura da soja. Entretanto, os resultados práticos dessa técnica são divergentes. Assim, pesquisas nesse campo são importantes para elucidar os efeitos diretos dessa prática de manejo, como, por exemplo, a interferência no uso da radiação fotossinteticamente ativa (RFA. Portanto, objetivou-se com este estudo avaliar a dinâmica da RFA em diferentes densidades de cultivo na cultura da soja. Para tanto, conduziu-se um experimento a campo com delineamento em blocos casualizados com os tratamentos distribuídos em arranjo fatorial 3 × 5: três cultivares de soja RR® (P98Y12, TMG 132 e M-Soy 9056 e cinco densidades de semeadura (20, 30, 40, 50 e 60 plantas∙m–2 com quatro repetições. Foram avaliados massa seca da parte aérea, radiação fotossinteticamente ativa no dossel inferior (RFA-I e superior (RFA-S, intercepção da RFA, taxa de crescimento da cultura (TCC, taxa de crescimento relativo (TCR, taxa de assimilação líquida (TAL, eficiência de uso da radiação (EUR, coeficiente de extinção luminosa (k, índice de colheita de grãos (ICG, peso de 1.000 grãos e produtividade. Independentemente do cultivar, as densidades de 20 e 30 plantas∙m–2 proporcionaram os maiores valores de TAL, TCR, k, ICG e produtividade. Apenas o uso dos parâmetros EUR, TCC e RFA interceptada não fornece informações sólidas para predizer a eficiência do cultivo adensado na cultura da soja. As densidades de cultivo tradicionalmente utilizadas se sobrepõem às demais quanto à qualidade da RFA no dossel e produtividade da soja.

  16. Variables socionutricionales de hogares mazahuas integrados por preescolares desnutridos con madres con obesidad y sin obesidad

    Directory of Open Access Journals (Sweden)

    Conzuelo González, Viridiana Vanessa

    2009-01-01

    Full Text Available El primer objetivo fue conocer cuántos menores de cinco años con diferentes grados de desnutrición tienen una madre con sobrepeso/obesidad/ en una comunidad indígena que vive en extrema pobreza y bajo condiciones de migración masculina internacional. El segundo fue comparar tres variables socionutricionales (ingreso familiar, educación de la madre y adecuación nutrimental de la dieta diaria entre estos hogares y los hogares con desnutrición infantil y madres sin obesidad. Se realizó un estudio transversal (2006-2007, en la comunidad mazahua de San Francisco Tepeolulco, Municipio de Temascalcingo; que incluyó a 85 hogares integrados por preescolares con desnutrición inscritos al programa Oportunidades. Se determinó el estado nutrición de los preescolares con indicadores antropométricos y se obtuvo el IMC de las madres de estos infantes. Se aplicó una encuesta socionutricional, incluida el recordatorio de 24 horas, y complementado con la observación participante (cualitativa. Se encontró que 83% de las madres mazahuas presentaron sobrepeso u obesidad. El estado de nutrición de los preescolares con madres con obesidad presentó un porcentaje mayor de desnutrición (76%. En la variable género, se encontró que 54% de los niños con madres con obesidad tenía baja talla. Al relacionar el nivel educativo de la madre, esta variable resultó ser estadísticamente significativa (p=0.015, donde el analfabetismo está más relacionado con la desnutrición infantil que tienen madres de bajo y/o peso normal. La elevada prevalencia de hogares conformados con preescolares con desnutrición y madres con obesidad, es un síntoma más de la pobreza en zonas indígenas en México, con bajo índice de desarrollo humano.

  17. Prevalência de pressão arterial elevada em crianças e adolescentes do ensino fundamental Prevalencia de presión arterial elevada en niños y adolescentes de la enseñanza fundamental Prevalence of elevated blood pressure in children and adolescents attending highschool

    Directory of Open Access Journals (Sweden)

    Ana Elisa M. Rinaldi

    2012-01-01

    Full Text Available OBJETIVO: Verificar a prevalência de pressão arterial elevada em crianças e adolescentes e sua associação com indicadores antropométricos. MÉTODOS: Estudo transversal de estudantes de três instituições de ensino em Botucatu (SP. As variáveis avaliadas foram: pressão arterial (PA (obtida em três ocasiões diferentes, peso, estatura, índice de massa corporal (IMC, circunferência braquial, circunferência abdominal (CA, dobras cutâneas tricipital e subescapular. A PA foi aferida por método auscultatório e classificada em pré-hipertensão (PH e hipertensão arterial (HAS, para os valores entre os percentis 90 e 95 e maior que o percentil 95, respectivamente. Os dados antropométricos foram comparados, segundo o sexo, pelo teste t de Student. A correlação de Pearson foi utilizada para verificar a variação das PA sistólica (PAS e diastólica (PAD segundo dados antropométricos. A variação do escore Z da PA segundo percentil de IMC foi avaliada pela análise de variância seguida do teste de Tukey. RESULTADOS: Foram avaliadas 903 crianças (51,7% meninos, com idade de 9,3±2,5 anos para ambos os sexos. A prevalência de PH foi de 9,1% e de HAS foi de 2,9%. Houve correlação positiva significativa entre os níveis de PAS e PAD elevados e as variáveis antropométricas, com valores maiores para peso (r=0,53 e r=0,45, pOBJETIVO: Verificar la prevalencia de presión arterial elevada en niños y adolescentes y su asociación con indicadores antropométricos. MÉTODOS: Estudio transversal incluyendo a estudiantes de tres instituciones de enseñanza de Botucatu (São Paulo, Brasil. Las variables evaluadas fueron: presión arterial (PA (obtenida en tres ocasiones distintas, peso, estatura, índice de masa corporal (IMC, circunferencia braquial, circunferencia abdominal (CA, pliegues cutáneos tricipital y subescapular. La PA fue verificada por método auscultatorio, siendo posteriormente clasificada como pre-hipertensión (PH e

  18. An automated multiplex specific IgE assay system using a photoimmobilized microarray.

    Science.gov (United States)

    Ito, Yoshihiro; Moritsugu, Nozomi; Matsue, Takahisa; Mitsukoshi, Kiyomi; Ayame, Hirohito; Okochi, Norihiko; Hattori, Hideshi; Tashiro, Hideo; Sato, Sakura; Ebisawa, Motohiro

    2012-11-15

    An automated microarray diagnostic system for specific IgE using photoimmobilized allergen has been developed. Photoimmobilization is useful for preparing microarrays, where various types of biological components are covalently immobilized on a plate. Because the immobilization is based on a photo-induced radical cross-linking reaction, it does not require specific functional groups on the immobilized components. Here, an aqueous solution of a photoreactive poly(ethylene glycol)-based polymer was spin-coated on a plate, and an aqueous solution of each allergen was microspotted on the coated plate and allowed to dry in air. Finally, the plate was irradiated with an ultraviolet lamp for covalent immobilization. An automated machine using these plates was developed for the assay of antigen-specific IgE. Initially, the patient serum was added to the microarray plate, and after reaction of the microspotted allergen with IgE, the adsorbed IgE was detected by a peroxidase-conjugated anti-IgE-antibody. The chemical luminescence intensity of the substrate decomposed by the peroxidase was automatically detected using a sensitive charge-coupled device camera. All the allergens were immobilized stably using this method, which was used to screen for allergen-specific IgE. The results were comparable with those using conventional specific IgE. Using this system, six different allergen-specific IgE were assayed using 10 μL of serum within a period of 20 min. Copyright © 2012 Elsevier B.V. All rights reserved.

  19. The property distance index PD predicts peptides that cross-react with IgE antibodies

    Science.gov (United States)

    Ivanciuc, Ovidiu; Midoro-Horiuti, Terumi; Schein, Catherine H.; Xie, Liping; Hillman, Gilbert R.; Goldblum, Randall M.; Braun, Werner

    2009-01-01

    Similarities in the sequence and structure of allergens can explain clinically observed cross-reactivities. Distinguishing sequences that bind IgE in patient sera can be used to identify potentially allergenic protein sequences and aid in the design of hypo-allergenic proteins. The property distance index PD, incorporated in our Structural Database of Allergenic Proteins (SDAP, http://fermi.utmb.edu/SDAP/), may identify potentially cross-reactive segments of proteins, based on their similarity to known IgE epitopes. We sought to obtain experimental validation of the PD index as a quantitative predictor of IgE cross-reactivity, by designing peptide variants with predetermined PD scores relative to three linear IgE epitopes of Jun a 1, the dominant allergen from mountain cedar pollen. For each of the three epitopes, 60 peptides were designed with increasing PD values (decreasing physicochemical similarity) to the starting sequence. The peptides synthesized on a derivatized cellulose membrane were probed with sera from patients who were allergic to Jun a 1, and the experimental data were interpreted with a PD classification method. Peptides with low PD values relative to a given epitope were more likely to bind IgE from the sera than were those with PD values larger than 6. Control sequences, with PD values between 18 and 20 to all the three epitopes, did not bind patient IgE, thus validating our procedure for identifying negative control peptides. The PD index is a statistically validated method to detect discrete regions of proteins that have a high probability of cross-reacting with IgE from allergic patients. PMID:18950868

  20. Linfoma de Burkitt primario de la cavidad oral en una paciente con sida. Reporte de un caso y revisión de la literatura

    Directory of Open Access Journals (Sweden)

    Marcelo Corti

    2015-01-01

    Full Text Available Los linfomas no Hodgkin (LNH son un grupo heterogéneo de enfermedades linfoproliferativas con elevada prevalencia en pacientes infectados por el virus de la inmunodeficiencia humana (VIH. La inmunodeficiencia asociada al sida predispone al desarrollo de LNH, incluyendo el linfoma de Burkitt (LB. El LB es un subtipo infrecuente y agresivo de LNH con elevada frecuencia en pacientes con sida. Se asocia a una alta tasa de replicación celular (determinada por el índice Ki67 y con alta frecuencia de compromiso extranodal como forma de presentación clínica de la neoplasia. Se presenta una paciente con sida que desarrolló un LB primario de la cavidad oral y se realiza una revisión de la literatura sobre el tema.

  1. Serological analysis of human IgG and IgE anti-insulin antibodies by solid-phase radioimmunoassays

    International Nuclear Information System (INIS)

    Hamilton, R.G.; Rendell, M.; Adkinson, N.F. Jr.

    1980-01-01

    A single solid-phase assay system which is useful for quantitative measurement of both IgG and IgE anti-insulin antibodies in human serum has been developed. Insulin-specific immunoglobulins are absorbed from human serum by excess quantities of insulin-agarose. After washes to remove unbound immunoglobulins, radioiodinated Staph A or rabbit anti-human IgE is added to detect bound IgG or IgE anbitodies, respectively

  2. Evidence of a common regulation of IgE and IgG-subclass antibodies in humans during immunotherapy

    DEFF Research Database (Denmark)

    Søndergaard, I; Poulsen, L K; Osterballe, O

    1992-01-01

    Based on a 3-year prospective study of 20 pollen-allergic patients, where a detailed analysis of the IgE, IgG1 and IgG4 immune response was performed, we propose that a common regulatory mechanism exists between the IgE and IgG1 synthesis and between IgE and IgG4 synthesis during immunotherapy. I...

  3. Elevada concentración de metabolitos de cotinina en hijos de padres fumadores High levels of cotinine metabolite in smoker's parents children

    Directory of Open Access Journals (Sweden)

    Eduardo Lazcano-Ponce

    2007-01-01

    Full Text Available INTRODUCCIÓN: La exposición al humo de tabaco de segunda mano (HTSM en niños y adultos puede ser común en oficinas gubernamentales, lugares de trabajo, vehículos y espacios públicos, pero es de particular importancia en el hogar. Los grados de exposición elevados en niños pueden constituirse como la principal justificación para restringir el tabaquismo dentro del hogar. El objetivo fue determinar los valores de HTSM mediante biomarcadores séricos en binomios padres- hijos menores de cinco años, en México, participantes de la Encuesta Nacional de Salud 2000. MATERIAL Y MÉTODOS: Se seleccionaron 76 binomios padres-hijos de hogares con adultos no fumadores y 83 binomios similares pero con adultos fumadores en el hogar. La selección se restringió a hogares donde hubiera presencia de niños menores de cinco años de edad. Las muestras séricas se analizaron por cromatografía líquida. Se construyeron modelos de correlación de exposición entre los binomios padres-hijos estratificados por el antecedente de tabaquismo en los padres. RESULTADOS: La prevalencia de individuos con más de 15 ng/mL de cotinina en suero en los fumadores fue de 100%; la cuantificación mínima fue 18.50 y la máxima de 221.5 ng/mL. En los adultos, los valores séricos de cotinina fueron 50 veces mayores en los fumadores (107.4 ng/mL que en los no fumadores (1.99 ng/mL; similar observación a la referida tuvo la exposición a 3’-hidroxicotinina (0.69 en no fumadores vs. 33.59 ng/mL en adultos fumadores. Hubo una diferencia significativa tres veces mayor de valores de cotinina (0.19 vs. 0.60 ng/mL y de 3’-hidroxicotinina (0.06 vs. 0.19 ng/mL en los hijos de fumadores. CONCLUSIONES: A pesar de que existe diferencia significativa de HTSM en niños de padres fumadores, la exposición observada de HTSM en niños menores de cinco años de padres no fumadores también es relevante. En México se documenta por primera vez una elevada exposición al HTSM en ni

  4. A soluble form of the high affinity IgE receptor, Fc-epsilon-RI, circulates in human serum.

    Directory of Open Access Journals (Sweden)

    Eleonora Dehlink

    Full Text Available Soluble IgE receptors are potential in vivo modulators of IgE-mediated immune responses and are thus important for our basic understanding of allergic responses. We here characterize a novel soluble version of the IgE-binding alpha-chain of Fc-epsilon-RI (sFcεRI, the high affinity receptor for IgE. sFcεRI immunoprecipitates as a protein of ∼40 kDa and contains an intact IgE-binding site. In human serum, sFcεRI is found as a soluble free IgE receptor as well as a complex with IgE. Using a newly established ELISA, we show that serum sFcεRI levels correlate with serum IgE in patients with elevated IgE. We also show that serum of individuals with normal IgE levels can be found to contain high levels of sFcεRI. After IgE-antigen-mediated crosslinking of surface FcεRI, we detect sFcεRI in the exosome-depleted, soluble fraction of cell culture supernatants. We further show that sFcεRI can block binding of IgE to FcεRI expressed at the cell surface. In summary, we here describe the alpha-chain of FcεRI as a circulating soluble IgE receptor isoform in human serum.

  5. Gambaran Igg4 dan Ige terhadap Protein Mikrofilaria pada Sera Penduduk Endemis Filariasis di Kecamatan Pasir Penyu, Riau

    OpenAIRE

    SU, Basundari; Kurniawan, Liliana; A., Soeroto; Marleta, Rita; Yasin, M

    1993-01-01

    Western blot test to detect specific IgG4 and IgE was performed to 12 microfilaraemic and 13 amicrofilaraemic individuals from malayan filariasis endemic area, Pasir Penyu, Riau. No differences in binding patterns of IgG4 and IgE antibodies to microfUarial protein components was shown. There was a parallel protein components recognition by IgG4 and IgE of molecular weight ranging from 158 kd to 14 kd. Protein component of 125 kd was only recognized by IgG4 and of 112 kd only by IgE. These fin...

  6. IgE epitopes of intact and digested Ara h 1

    DEFF Research Database (Denmark)

    Bøgh, Katrine Lindholm; Nielsen, H.; Madsen, Charlotte Bernhard

    2012-01-01

    epitopes have been suggested to be of great importance. ObjectiveThe aim of this study was to identify IgE specific epitopes of intact and digested Ara h 1, and to compare epitope patterns between humans and rats. MethodsSera from five peanut allergic patients and five Brown Norway rats were used...... to identify intact and digested Ara h 1-specific IgE epitopes by competitive immunoscreening of a phage-displayed random hepta-mer peptide library using polyclonal IgE from the individual sera. The resulting peptide sequences were mapped on the surface of a three-dimensional structure of the Ara h 1 molecule...... to mimic epitopes using a computer-based algorithm. ResultsPatients as well as rats were shown to have individual IgE epitope patterns. All epitope mimics were conformational and found to cluster into three different areas of the Ara h 1 molecule. Five epitope motifs were identified by patient IgE, which...

  7. Differential induction of total IgE by two Salmonella enterica serotypes

    Directory of Open Access Journals (Sweden)

    Zhanna eKtsoyan

    2015-06-01

    Full Text Available The main goal of this study was to establish how the inflammation caused by infection with two different Salmonella enterica serotypes, S. Typhimurium and S. Enteritidis, may lead to the predisposition to allergy as measured by total IgE level in the blood. Infection by S. Typhimurium did not affect the systemic IgE concentration while in S. Enteritidis-infected patients there was a significant 3.5-fold increase. This effect was especially profound in patients >4 years old, with up to the eight-fold increase above the norm. The degree of dysbiosis in these two infections measured with the comparative counts of cultivated bacteria showed an inverse relationship with the IgE concentration. Earlier we reported the elevated level of IL-17 in patients infected by S. Enteritidis. In the current study a significant correlation was found between the concentrations of IL-17 and IgE suggesting a possible role played by this cytokine in triggering the production of IgE in response to S. Enteritidis infection.

  8. Hyper IgE in Childhood Eczema and Risk of Asthma in Chinese Children.

    Science.gov (United States)

    Ng, Chantel; Hon, Kam Lun; Kung, Jeng Sum Charmaine; Pong, Nga Hin; Leung, Ting-Fan; Wong, Chun Kwok

    2016-06-10

    Atopic eczema is a common childhood disease associated with high IgE and eosinophilia. We characterized the clinical features associated with hyper-IgE (defined as IgE > 2000 IU/L) in eczema. Nottingham Eczema Severity Score (NESS), family and personal history of atopy, skin prick test (SPT) for common food and aeroallergens, highest serum IgE ever and eosinophil counts were evaluated in 330 children eczema patients. Childhood-NESS (NESS performed at 10 years of age) were further analyzed. IgE correlated with NESS (spearman coefficient 0.35, p asthma (p childhood (p asthma (exp(B) = 5.12, p = 0.002) and eczema severity during childhood and adolescence (p 10years of age, food allergen sensitization was associated with hyper-IgE (p = 0.008). Hyper-IgE is independently associated with asthma, more severe atopy and more severe eczema during childhood and adolescence. IgE > 2000 IU/L may be a tool to aid prognostication of this chronic relapsing dermatologic disease and its progression to asthma.

  9. Exploring the temporal development of childhood IgE profiles to allergen components

    Directory of Open Access Journals (Sweden)

    Önell Annica

    2012-12-01

    Full Text Available Abstract Background Children often develop allergies that may or not persist into adulthood. Although the different allergic symptoms over time have been well documented, the underlying pattern of sensitization to various proteins and subsequent allergy development is unexplored. The aim was to study the sensitization pattern to allergen components over time from infancy to adulthood in a group of infants with heredity for allergic diseases. Methods IgE profiles were monitored in a group of 67 children from 6 months to 18 years using a microarray chip (ImmunoCAP® ISAC containing 103 allergen components derived from 47 allergen sources. The chip IgE profile was compared with clinical history, skin prick test results and diagnoses (atopic dermatitis, asthma and allergic rhinoconjunctivitis at each time point for each child. Results IgE profiles were unique for each child and showed broad agreement with the results of skin prick tests and doctors’ diagnoses. In addition, close examination of the IgE profiles often revealed early indication of subsequent allergies. IgE profiles also facilitated the examination of cross-reactivity contra co-sensitization, thereby greatly enhancing the possibility for managing patients. Conclusion This explorative description indicates that sensitization pattern to allergen components differs over time as well as among allergic individuals when examined with microarray technology.

  10. IgE Sensitization to Bacterial and Fungal Biopesticides in a Cohort of Danish Greenhouse Workers

    DEFF Research Database (Denmark)

    Doekes, G.; Larsen, Preben; Sigsgaard, T.

    2004-01-01

    BACKGROUND: The use of biopesticides in agriculture may implicate new risks of work-related allergic reactions. METHODS: Sera were tested from the BIOGART project, a longitudinal respiratory health study among >300 Danish greenhouse workers. IgE was measured by enzyme immunoassay (EIA) with extra......BACKGROUND: The use of biopesticides in agriculture may implicate new risks of work-related allergic reactions. METHODS: Sera were tested from the BIOGART project, a longitudinal respiratory health study among >300 Danish greenhouse workers. IgE was measured by enzyme immunoassay (EIA......) with extracts of biopesticide products containing Bacillus thuringiensis (BT) or Verticillium lecanii (Vert). RESULTS: Many sera had detectable IgE to BT (23-29%) or Vert (9-21%). IgE titers from the 2- and 3-year follow-up (n=230) were highly correlated, with discordant results in ... BT, or to different Verticillium products were also significantly correlated (both r >0.70), whereas IgE anti-BT and anti-Verticillium showed no correlation at all. CONCLUSIONS: Exposure to these microbial biopesticides may confer a risk of IgE-mediated sensitization. In future research...

  11. High-Dose Ionizing Radiation Photographic Dosimetry; Mesure, par des Methodes Photogra Phiques, de Doses Elevees de Rayonnements Ionisants; 0424 041e 0422 041e 0413 0420 0414 ; Dosimetria Fotografica de Dosis Elevadas de Radiaciones Ionizantes

    Energy Technology Data Exchange (ETDEWEB)

    Trousil, J.; Bucina, I. [Institute for Research Production and Application of Radioisotopes Prague, Czechoslovakia (Czech Republic)

    1965-06-15

    ionizantes comprendidas entre 10 mr y 1000 r (para rayos gamma y rayos X de elevada energia). Con arreglo a este metodo, se utilizan dos peliculas con emulsiones diferentes, montadas en el mismo estuche. El metodo se caracteriza esencialmente por el hecho de que permite evaluar dosis comprendidas entre los limites indicados, sin necesidad de saber si se produjo una exposicion a dosis mas elevadas, con un margen de error inferior a {+-}25 en todo el intervalo (limite de seguridad 95%); ademas permite determinar con una precision satisfactoria la energia de las radiaciones a que la pelicula estuvo expuesta. Se examinan los aparatos, peliculas y tratamiento quimicos necesarios para esas mediciones. Se explica el principio de construccion de un densitometro que permite medir el ennegrecimiento de la pelicula con una precision constante en el intervalo comprendido entre D = 0 y D = 6. De este modo es posible aprovechar toda la zona de ennegrecimiento de la pelicula correspondiente a dicho intervalo. Como no se utiliza iotomultiplicador, los riesgos de iluminacion excesiva se reducen al minimo, por lo que el aparato resulta apropiado para mediciones corrientes. Ademas se indican los parametros que han de satisfacer las peliculas dosimetricas para poder detectar dosis elevadas, y se comparan las caracteristicas de las peliculas dosimetricas FOMA y ORWO en lo que respecta a la medicion de dosis elevadas. Con un progreso en la dosimetria de dosis elevadas, cabe mencionar el metodo de revelado retardado que se describe con detalle, con arreglo al cual se anaden al revelador compuestos del tipo nitrobenzimidazol y benzotriazol, en concentracion adecuada. En el caso de emulsiones lentas con elevado contenido de plata, esta tecnica permite medir exposiciones de dosis gamma (rayos X de elevada energia) de hasta 10{sup 3}r, o aun mayores, si no es menester una evaluacion precisa en un intervalo de hasta 100 r. Se describe el efecto retardador de los productos quimicos anadidos al revelador

  12. Ferropenia en niños de 6 a 24 meses de edad con hemoglobina normal

    Directory of Open Access Journals (Sweden)

    Marlen Ruiz González

    2002-04-01

    Full Text Available Se realizó un estudio a 55 niños de 6 a 24 meses de edad con hemoglobina normal con el objetivo de detectar precozmente la ferropenia y de identificar los factores que pueden contribuir a su aparición. Para esto se utilizó la determinación de protoporfirina eritrocitaria libre (PEL por punción digital. Teniendo en cuenta que esta no solo se eleva en la ferropenia, sino también en otras patologías como la intoxicación plúmbica, se le realizó a todo niño con protoporfirina elevada, coproporfirina III y ácido delta amino levulínico (ALA en orina. Además, se aplicó una encuesta donde se recogieron los posibles factores que de alguna forma pudieran provocar la ferropenia. Se encontraron 16 niños (29,1 % con PEL elevada, que coincidieron con el uso de lactancia artificial o mixta, exceso en la ingestión de leche y una alimentación inadecuada. Se concluyó que la PEL es una prueba útil para detectar la ferropenia en etapas tempranas, y que esta se relaciona con hábitos alimentarios incorrectos, lo cual puede ser solucionado con una mejor orientación a nivel de la Atención Primaria.

  13. IgE sensitization to inhalant allergens and the risk of airway infection and disease

    DEFF Research Database (Denmark)

    Skaaby, Tea; Husemoen, Lise Lotte Nystrup; Thuesen, Betina Heinsbæk

    2017-01-01

    .26, 95% CI: 1.79, 2.86), and other chronic lower airway disease (HR = 1.31, 95% CI: 1.08, 1.58). In never smokers, the higher risk of pneumonia (HR = 1.73, 95% CI: 1.23, 2.44) and asthma (HR = 3.17, 95% CI: 2.10, 4.76) among IgE sensitized was more pronounced. CONCLUSIONS: IgE sensitization......-analysed, and expressed as hazard ratios, HRs (95% confidence intervals, CIs). RESULTS: The relative risks for IgE sensitized vs. non-sensitized were: for pneumonia (HR = 1.20, 95% CI: 1.01, 1.41), other acute airway infection (HR = 0.86, 95% CI: 0.60, 1.22), infection (HR = 1.06, 95% CI: 0.90, 1.24), asthma (HR = 2...

  14. Human IgE is efficiently produced in glycosylated and biologically active form in lepidopteran cells

    DEFF Research Database (Denmark)

    Bantleon, Frank; Wolf, Sara; Seismann, Henning

    2016-01-01

    the recombinant production of the highly complex IgE isotype in insect cells. Recombinant IgE (rIgE) was efficiently assembled and secreted into the supernatant in yields of >30 mg/L. Purification from serum free medium using different downstream processing methods provided large amounts of rIgE. This exhibited...... a highly specific interaction with its antigen, therapeutic anti-IgE and its high affinity receptor, the FcεRI. Lectins and glyco-proteomic analyses proved the presence of prototypic insect type N-glycans on the epsilon heavy chain. Mediator release assays demonstrated a biological activity of the r......IgE comparable to IgE derived from mammalian cells. In summary the expression in insect cells provides rIgE with variant glycosylation pattern, but retained characteristics and biological activity. Therefore our data contribute to the understanding of functional and structural aspects and potential use of the Ig...

  15. Helminths and malaria co-infections are associated with elevated serum IgE

    DEFF Research Database (Denmark)

    Mulu, Andargachew; Kassu, Afework; Legesse, Mengistu

    2014-01-01

    BACKGROUND: Both helminth and malaria infections result in a highly polarized immune response characterized by IgE production. This study aimed to investigate the total serum IgE profile in vivo as a measure of Th2 immune response in malaria patients with and without helminth co-infection. METHODS......: A cross sectional observational study composed of microscopically confirmed malaria positive (N = 197) and malaria negative (N = 216) apparently healthy controls with and without helminth infection was conducted at Wondo Genet Health Center, Southern Ethiopia. A pre-designed structured format was utilized...... to collect socio-demographic and clinical data of the subjects. Detection and quantification of helminths, malaria parasites and determination of serum IgE levels were carried out following standard procedures. RESULTS: Irrespective of helminth infection, individuals infected by malaria showed significantly...

  16. Aspergillus specific IgE estimation by radioallergosorbent technique (RAST) in obstructive airways disease at Agra

    International Nuclear Information System (INIS)

    Sharma, S.K.; Singh, R.; Mehrotra, M.P.; Patney, N.L.; Sachan, A.S.; Shiromany, A.

    1986-01-01

    The radioallergosorbent technique (RAST) was used to measure the levels of Aspergillus specific IgE in 25 normal controls, 25 cases of extrinsic bronchial asthma and 25 cases of allergic broncho-pulmonary aspergillosis with a view to study the clinical role and its correlation with sputum culture, skin sensitivity and severity of airways obstruction. The test was performed using Pharmacia diagnostic kits with antigen derived from Aspergillus fumigatus. Abnormal levels of Aspergillus specific IgE were observed in 84 per cent cases of bronchial asthma but none of the controls. 86.7 per cent of all cases with positive skin test had positive radioallergosorbent test and there was no false positive reaction. There was a positive correlation of Aspergillus specific IgE with skin test positivity and with FEV 1 /FVC per cent. (author)

  17. Helminth allergens, parasite-specific IgE and its protective role in human immunity

    Directory of Open Access Journals (Sweden)

    Colin Matthew Fitzsimmons

    2014-02-01

    Full Text Available The Th2 immune response, culminating in eosinophilia and IgE production, is not only characteristic of allergy but also of infection by parasitic worms (helminths. Anti-parasite IgE has been associated with immunity against a range of helminth infections and many believe that IgE and its receptors evolved to help counter metazoan parasites. Allergens (IgE-antigens are present in only a small minority of protein families and known IgE targets in helminths belong to these same families (e.g. EF-hand proteins, tropomyosin, and PR-1 proteins.During some helminth infection, especially with the well adapted hookworm, the Th2 response is moderated by parasite-expressed molecules. This has been associated with reduced allergy in helminth endemic areas and worm infection or products have been proposed as treatments for allergic conditions. However some infections (especially Ascaris are associated with increased allergy and this has been linked to cross-reactivity between worm proteins (e.g., tropomyosins and highly similar molecules in dust mites and insects. The overlap between allergy and helminth infection is best illustrated in Anisakis simplex, a nematode that when consumed in under-cooked fish can be both an infective helminth and a food allergen. Nearly 20 molecular allergens have been isolated from this species, including tropomyosin (Ani s3 and the EF-hand protein, Ani s troponin.In this review, we highlight aspects of the biology and biochemistry of helminths that may have influenced the evolution of the IgE response. We compare dominant IgE antigens in worms with clinically important environmental allergens and suggest that arrays of such molecules will provide important information on anti-worm immunity as well as allergy.

  18. Efeitos hemodinâmicos e renais da injeção de doses elevadas de clonidina no espaço peridural do cão Efectos hemodinámicos y renales de la inyección de dosis elevadas de clonidina en el espacio peridural del perro Hemodynamic and renal effects of high epidural clonidine doses in dogs

    Directory of Open Access Journals (Sweden)

    Nilson Camargo Roso

    2005-06-01

    ébito cardíaco e aumento da relação depuração de para-aminohipurato de sódio/débito cardíaco. CONCLUSÕES: Nas condições realizadas e nas doses empregadas, pode-se concluir que a clonidina não promoveu alteração da função renal, mas diminuiu valores hemodinâmicos (freqüência e débito cardíaco.JUSTIFICATIVA Y OBJETIVOS: No existen estudios que relaten las repercusiones renales determinadas por la inyección de dosis elevadas de clonidina en el espacio peridural. La finalidad del estudio fue evaluar los efectos hemodinámicos y renales determinados por la inyección de dosis elevadas de clonidina en el espacio peridural del perro. MÉTODO: Veinte animales anestesiados con tiopental sódico y fentanil fueron distribuidos eventualmente y de forma doblemente encubierta en dos grupos: Grupo 1 ó placebo (n = 10, que recibió 0,2 mL.kg-1 de solución fisiológica, y Grupo 2 ó clonidina (n = 10, que recibió 0,2 mL.kg-1 de una solución conteniendo 50 µg.mL-1 de clonidina, en el espacio peridural. Fueron evaluados los siguientes parámetros hemodinámicos: frecuencia cardiaca (FC: lat.min-1; presión arterial media (PAM: mmHg; presión de la arteria pulmonar ocluida (PAOP: mmHg; débito cardíaco (DC: L.min-1; volumen sistólico (VS: mL; también, los siguientes parámetros de la función renal fueron evaluados: flujo sanguíneo renal (FSR: mL.min-1; resistencia vascular renal (RVR: mmHg.mL-1.min; volumen urinario minuto (VUM: mL.min-1; depuración de creatinina (DCr: mL.min-1; depuración de para-aminohipurato (DPAH: mL.min-1; fracción de filtración (FF; depuración de sodio (DNa: mL.min-1; depuración de potasio (DK: mL.min-1; excreción fraccionaria de sodio (EFNa: %; excreción urinaria de sodio (UNaV: µEq.min-1; excreción urinaria de potasio (UKV: µEq.min-1. El experimento consistió en tres momentos de 20 minutos cada uno. Los datos fueron colectados a los 10 minutos de cada momento y la diuresis, en el inicio y al final de cada momento. Al t

  19. Primary sclerosing cholangitis associated with increased peripheral eosinophils and serum IgE.

    Science.gov (United States)

    Shimomura, I; Takase, Y; Matsumoto, S; Kuyama, J; Nakajima, T; Maeda, H; Sugase, T; Hata, A; Hanada, M; Okuno, M

    1996-10-01

    Symptoms of cholestasis, including epigastralgia, fever, and jaundice, with marked increases in peripheral eosinophils and serum IgE in a 20-year-old man are reported here. Endoscopic retrograde cholangio-pancreatography (ERCP) detected constrictions of the bile ducts, compatible with primary sclerosing cholangitis (PSC). The symptoms and blood parameters of liver dysfunction were associated with the degree of eosinophilia and high serum IgE levels. During corticosteroid therapy, all of these parameters improved, and morphologic improvements of the bile ducts were also observed. The pathogenesis of PSC may be explained, in part, by the concept of hypereosinophilic syndrome or allergic reaction.

  20. Rare association of hyper IgE syndrome with cervical rib and natal teeth

    Directory of Open Access Journals (Sweden)

    Roshan Anupama

    2009-01-01

    Full Text Available Hyper IgE syndrome (HIES is a rare immunodeficiency syndrome characterized by a triad of cutaneous abscesses, mostly caused by Staphylococus aureus; pneumonia; and raised IgE levels. Nonimmunological associations include course facial features, multiple bone fractures, joint hyperextensibility, and retained primary dentition. Patients require long-term antibiotic therapy. We report here a classical case of HIES with rare associations of natal teeth, bilateral cervical ribs, and conductive deafness. The patient was being treated with monteleukast and dapsone.

  1. Fournier gangrene associated with hyper IgE syndrome (Job syndrome).

    Science.gov (United States)

    Hori, Junichi; Yamaguchi, Satoshi; Watanabe, Masaki; Osanai, Hiroaki; Hori, Masako

    2008-04-01

    We report a case of a 32-year-old man with hyper IgE syndrome (Job syndrome) who developed Fournier gangrene due to infectious multiple atheromas of the scrotal skin that progressed to the right groin and thigh. The patient required surgical debridement and subsequent skin grafting. This is a rare case of Fournier gangrene associated with hyper IgE syndrome (Job syndrome). When a patient without diabetes mellitus has repeated infections and atopic-like dermatitis, Job syndrome should be considered.

  2. Syntaxin-4 is essential for IgE secretion by plasma cells

    Energy Technology Data Exchange (ETDEWEB)

    Rahman, Arman; DeCourcey, Joseph; Larbi, Nadia Ben [Immunomodulation Group, School of Biotechnology, Dublin City University (Ireland); Loughran, Sinéad T.; Walls, Dermot [School of Biotechnology and National Centre for Sensor Research, Dublin City University (Ireland); Loscher, Christine E., E-mail: christine.loscher@dcu.ie [Immunomodulation Group, School of Biotechnology, Dublin City University (Ireland)

    2013-10-11

    Highlights: •Knock-down of syntaxin-4 in U266 plasma cells resulted in reduction of IgE secretion. •Knock-down of syntaxin-4 also leads to the accumulation of IgE in the cell. •Immuno-fluorescence staining shows co-localisation of IgE and syntaxin-4 in U266 cells. •Findings suggest a critical requirement for syntaxin-4 in IgE secretion from plasma cells. -- Abstract: The humoral immune system provides a crucial first defense against the invasion of microbial pathogens via the secretion of antigen specific immunoglobulins (Ig). The secretion of Ig is carried out by terminally differentiated B-lymphocytes called plasma cells. Despite the key role of plasma cells in the immune response, the mechanisms by which they constitutively traffic large volumes of Ig out of the cell is poorly understood. The involvement of Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins in the regulation of protein trafficking from cells has been well documented. Syntaxin-4, a member of the Qa SNARE syntaxin family has been implicated in fusion events at the plasma membrane in a number of cells in the immune system. In this work we show that knock-down of syntaxin-4 in the multiple myeloma U266 human plasma cell line results in a loss of IgE secretion and accumulation of IgE within the cells. Furthermore, we show that IgE co-localises with syntaxin-4 in U266 plasma cells suggesting direct involvement in secretion at the plasma membrane. This study demonstrates that syntaxin-4 plays a critical role in the secretion of IgE from plasma cells and sheds some light on the mechanisms by which these cells constitutively traffic vesicles to the surface for secretion. An understanding of this machinery may be beneficial in identifying potential therapeutic targets in multiple myeloma and autoimmune disease where over-production of Ig leads to severe pathology in patients.

  3. Syntaxin-4 is essential for IgE secretion by plasma cells

    International Nuclear Information System (INIS)

    Rahman, Arman; DeCourcey, Joseph; Larbi, Nadia Ben; Loughran, Sinéad T.; Walls, Dermot; Loscher, Christine E.

    2013-01-01

    Highlights: •Knock-down of syntaxin-4 in U266 plasma cells resulted in reduction of IgE secretion. •Knock-down of syntaxin-4 also leads to the accumulation of IgE in the cell. •Immuno-fluorescence staining shows co-localisation of IgE and syntaxin-4 in U266 cells. •Findings suggest a critical requirement for syntaxin-4 in IgE secretion from plasma cells. -- Abstract: The humoral immune system provides a crucial first defense against the invasion of microbial pathogens via the secretion of antigen specific immunoglobulins (Ig). The secretion of Ig is carried out by terminally differentiated B-lymphocytes called plasma cells. Despite the key role of plasma cells in the immune response, the mechanisms by which they constitutively traffic large volumes of Ig out of the cell is poorly understood. The involvement of Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins in the regulation of protein trafficking from cells has been well documented. Syntaxin-4, a member of the Qa SNARE syntaxin family has been implicated in fusion events at the plasma membrane in a number of cells in the immune system. In this work we show that knock-down of syntaxin-4 in the multiple myeloma U266 human plasma cell line results in a loss of IgE secretion and accumulation of IgE within the cells. Furthermore, we show that IgE co-localises with syntaxin-4 in U266 plasma cells suggesting direct involvement in secretion at the plasma membrane. This study demonstrates that syntaxin-4 plays a critical role in the secretion of IgE from plasma cells and sheds some light on the mechanisms by which these cells constitutively traffic vesicles to the surface for secretion. An understanding of this machinery may be beneficial in identifying potential therapeutic targets in multiple myeloma and autoimmune disease where over-production of Ig leads to severe pathology in patients

  4. Prediction of allergy from family history and cord blood IgE levels. A follow-up at the age of 5 years. Cord blood IgE. IV

    DEFF Research Database (Denmark)

    Hansen, L G; Halken, S; Høst, A

    1993-01-01

    was not influenced by cord blood IgE levels or atopic predisposition. Cord blood IgE levels had a low sensitivity as a predictor of atopic disease. A statistically significant correlation between serum levels of IgE at birth and at 5 years was however found (p ... with elevated cord blood IgE levels developed allergic disease before 5 years of age (p 63 kU/l (geometric mean + 1 SD) at the age of 5 years can be regarded as being an elevated level. A cord...... blood IgE level > or = 0.3 kU/l in combination with atopic predisposition was predictive of allergic disease, especially allergic bronchial asthma. With regard to allergic disease, the positive predictive value was 26%, the sensitivity 33% and the rate ratio for development of allergic disease 4...

  5. Elevada prevalencia de Cyclospora cayetanensis en indígenas del estado Bolívar, Venezuela High prevalence of Cyclospora cayetanensis among indigenous people in Bolivar State, Venezuela

    Directory of Open Access Journals (Sweden)

    Rodolfo Devera

    2005-12-01

    Full Text Available En la actualidad las coccidiosis intestinales causadas por Cryptosporidium parvum, Isospora belli y Cyclospora cayetanensis constituyen parasitosis de gran importancia médica, sin embargo, en el Estado Bolívar, Venezuela, se desconocen muchos aspectos epidemiológicos de estas infecciones. Entre julio de 2003 y abril de 2004 fue evaluada la población indígena San Antonio de Morichal con el objetivo de determinar la prevalencia de coccidiosis intestinales. Las muestras fecales obtenidas por evacuación espontánea fueron preservadas en dicromato de potasio 2,5% y posteriormente examinadas mediante la técnica de concentración con formol-éter y coloración de Kinyoun. De los 160 indígenas evaluados, el 92,5% (148 casos estaba parasitado. Entamoeba coli (58,8%, Ascaris lumbricoides (38,8% y Giardia lamblia (18,8% fueron los más comunes. La prevalencia de coccidiosis intestinal fue de 13,1%, siendo la cyclosporiosis la más importante con 11,9% (19 casos. Solamente se diagnosticó un caso con ooquistes de C. parvum (0,6% y otro con I. belli (0,6%. No hubo diferencias en cuanto al sexo (p > 0,05 y la edad (c2 = 5,6; g.l: 6 de los habitantes infectados con C. cayetanensis. De los infectados con C. cayetanensis únicamente dos tenían diarrea. En conclusión, se determinó una prevalencia elevada de coccidiosis intestinal en habitantes de la comunidad indígena evaluada, siendo la infección por C. cayetanensis las más prevalente.The intestinal coccidioses caused by Cryptosporidium parvum, Isospora belli, and Cyclospora cayetanensis are parasitoses of major medical importance, but many epidemiological aspects of these infections are still unknown in Bolívar State, Venezuela. To determine the prevalence of intestinal parasites and especially of intestinal coccidiosis, an indigenous population in San Antonio de Morichal was evaluated from July 2003 to April 2004. Stool samples obtained by spontaneous evacuation were preserved in potassium

  6. Recurrent Skin and Lung Infections in Autosomal Dominant Hyper IgE Syndrome with Transactivation Domain STAT3 Mutation

    Directory of Open Access Journals (Sweden)

    Chad J. Cooper

    2014-01-01

    Full Text Available Background. Hyper IgE is a rare systemic disease characterized by the clinical triad of high serum levels of IgE (>2000 IU/mL, eczema, and recurrent staphylococcal skin and lung infections. The presentation of hyper IgE syndrome is highly variable, which makes it easy to confuse the diagnosis with that of severe atopy or other rare immunodeficiency disorders. Case Report. A 23-year-old Hispanic presented with history of frequent respiratory and gastrointestinal infections as a child and multiple episodes of skin and lung infections (abscess with Staphylococcus aureus throughout his adult life. He had multiple eczematous lesions and folliculitis over his entire body, oral/esophageal candidiasis, and retention of his primary teeth. The IgE was elevated (>5000 IU/mL. Genetic mutation analysis revealed a mutation affecting the transactivation domain of the STAT3 gene. Conclusion. The hallmark of hyper IgE syndrome is serum IgE of >2000 IU/mL. Hyper IgE syndrome is a genetic disorder that is either autosomal dominant or recessive. A definite diagnosis can be made with genetic mutation analysis, and in this case, it revealed a very rare finding of the transactivation domain STAT3 mutation. Hyper IgE syndrome is a challenge for clinicians in establishing a diagnosis in suspected cases.

  7. Molecular Allergen-Specific IgE Assays as a Complement to Allergen Extract-Based Sensitization Assessment

    NARCIS (Netherlands)

    Aalberse, Rob C.; Aalberse, Joost A.

    2015-01-01

    Molecular allergen-based component-resolved diagnostic IgE antibody tests have emerged in the form of singleplex assays and multiplex arrays. They use both native and recombinant allergen molecules, sometimes in combination with each other, to supplement allergen extract-based IgE antibody analyses.

  8. Rhinitis symptoms and IgE sensitization as risk factors for development of later allergic rhinitis in adults

    DEFF Research Database (Denmark)

    Bødtger, Uffe; Poulsen, L K; Linneberg, A

    2006-01-01

    -examined in 1998. On both occasions questionnaires on rhinitis symptoms were completed and serum IgE (against birch, grass, mugwort, cat, dog, and Dermatophagoides pteronyssinus) were determined (positive if >or=0.35 kUA/l). Asymptomatic sensitization: positive IgE levels without any rhinitis symptoms. Nonallergic...

  9. Desirability and Implementation of IGE/Secondary Schooling in Selected Innovative High Schools. Tehnical Report No. 493.

    Science.gov (United States)

    Maier, Michael John

    The purposes of this study were to assess staff perceptions of desirability and level of implementation of Individually Guided Education (IGE)/Secondary Schooling in four high schools implementing programs similar to IGE and to identify factors enhancing or impeding implementation of these programs. Theoretical foundations of the study included…

  10. Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids

    DEFF Research Database (Denmark)

    Poulsen, L K; Pedersen, M F; Malling, H J

    1989-01-01

    For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ("Maxisorp RAST"). Cladosporium herbarum extract was used as a model allergen...

  11. Seroprevalencia de infección por virus de la hepatitis B y por virus de la inmunodeficiencia humana en una población de pacientes con múltiples transfusiones en cuatro hospitales, Colombia, Sur América

    Directory of Open Access Journals (Sweden)

    Mauricio Beltrán

    2009-06-01

    Conclusiones. Este es el primer estudio epidemiológico realizado en Colombia con un número significativo de pacientes con múltiples transfusiones. El hallazgo más relevante es la elevada prevalencia de infección por virus de la hepatitis B y virus de la inmunodeficiencia humana observado en la población de hemofílicos, comparado con los otros cuatro grupos de pacientes con múltiples transfusiones.

  12. The prevalence and identity of Chlamydia-specific IgE in children with asthma and other chronic respiratory symptoms

    Directory of Open Access Journals (Sweden)

    Patel Katir K

    2012-04-01

    Full Text Available Abstract Background Recent studies have confirmed the presence of viable Chlamydia in the bronchoalveolar lavage (BAL fluid of pediatric patients with airway hyperresponsiveness. While specific IgG and IgM responses to C. pneumoniae are well described, the response and potential contribution of Ag-specific IgE are not known. The current study sought to determine if infection with Chlamydia triggers the production of pathogen-specific IgE in children with chronic respiratory diseases which might contribute to inflammation and pathology. Methods We obtained BAL fluid and serum from pediatric respiratory disease patients who were generally unresponsive to corticosteroid treatment as well as sera from age-matched control patients who saw their doctor for wellness checkups. Chlamydia-specific IgE was isolated from BAL and serum samples and their specificity determined by Western blot techniques. The presence of Chlamydia was confirmed by species-specific PCR and BAL culture assays. Results Chlamydial DNA was detected in the BAL fluid of 134/197 (68% patients. Total IgE increased with age until 15 years old and then decreased. Chlamydia-specific IgE was detected in the serum and/or BAL of 107/197 (54% patients suffering from chronic respiratory disease, but in none of the 35 healthy control sera (p p = 0.0001 tested positive for Chlamydia-specific IgE. Asthmatic patients had significantly higher IgE levels compared to non-asthmatics (p = 0.0001. Patients who were positive for Chlamydia DNA or culture had significantly higher levels of serum IgE compared to negative patients (p = 0.0071 and p = 0.0001 respectively. Only 6 chlamydial antigens induced Chlamydia-specific IgE and patients with C. pneumoniae-specific IgE had significantly greater levels of total IgE compared to C. pneumoniae-specific IgE negative ones (p = 0.0001. Conclusions IgE antibodies play a central role in allergic inflammation; therefore production of Chlamydia

  13. The changes of IL-8, IL-10, IL-12 and IgE in serum of patients with asthma

    International Nuclear Information System (INIS)

    Zhang Chao; Liu Deyi; Hou Guihua; Wang Haodan

    2002-01-01

    To evaluate the relationship and the clinical significance between the serum IL-8, IL-10, IL-12 and IgE in patients with asthma, the serum IL-8, IL-10 are measured by radioimmunoassay method and the serum IL-12, IgE by ELISA in 55 patients with asthma. The level of serum IL-8, IgE at stage of episode are significantly higher than that at stage of remission (P<0.01); the level of serum IL-10, IL-12 at stage of episode are significantly lower than that at stage of remission (P<0.01). Linear regression shows that the decrease of IL-12 relate to the increase of IgE. The results suggests that the change of the level of serum IL-8, IL-10, IL-12 and IgE could be a maker for the aggravation of asthma

  14. Total IgE and eotaxin (CCL11) contents in tears of patients suffering from seasonal allergic conjunctivitis.

    Science.gov (United States)

    Eperon, Simone; Berguiga, Marouen; Ballabeni, Pierluigi; Guex-Crosier, Catherine; Guex-Crosier, Yan

    2014-09-01

    To prospectively investigate patients with seasonal allergic conjunctivitis (SAC) during the pollen season and test associations between tears total IgE, eotaxin concentrations, and SAC severity. Enrolled patients presented ocular symptoms and clinical signs of SAC at the time of presentation. Ocular itching, hyperaemia, chemosis, eyelid swelling, and tearing were scored, and the sum of these scores was defined as the clinical score. Conjunctival papillae were separately graded. We measured eotaxin concentration in tears by an enzyme-linked immunosorbent assay (ELISA) and total tear IgE by Lacrytest strip. Among thirty patients (30 eyes), 11 showed neither tear IgE nor tear eotaxin, while 15 out of 19 patients with positive IgE values presented a positive amount of eotaxin in their tears (Fisher's test: p tear IgE, we observed a lower conjunctival papilla grade than in patients whose tears contained some amount of IgE (trend test: p = 0.032). In the 15 patients whose tear eotaxin concentration was null, tear IgE concentration was 5.3 ± 3.5 arbitrary units; in the other 15 patients whose eotaxin was positive, IgE reached 21 ± 4.3 arbitrary U (Mann-Whitney: p tear IgE concentrations of both groups did not differ statistically significantly (p = 0.947). If IgE and eotaxin secreted in tears are major contributors in SAC pathogenesis, they however act at different steps of the process.

  15. Screening for hen's egg and chicken meat specific IgE antibodies in ...

    African Journals Online (AJOL)

    Background: Allergy to hen's egg and meat contributes significantly to the manifestations of food allergy all over the world. Objectives: This study was performed to assess the presence of hen's egg and meat specific IgE antibodies among patients investigated for various allergic disorders. Methods. This is a retrospective ...

  16. Omalizumab reduces bronchial mucosal IgE and improves lung function in non-atopic asthma.

    Science.gov (United States)

    Pillai, Prathap; Chan, Yih-Chih; Wu, Shih-Ying; Ohm-Laursen, Line; Thomas, Clare; Durham, Stephen R; Menzies-Gow, Andrew; Rajakulasingam, Raj K; Ying, Sun; Gould, Hannah J; Corrigan, Chris J

    2016-12-01

    Omalizumab therapy of non-atopic asthmatics reduces bronchial mucosal IgE and inflammation and preserves/improves lung function when disease is destabilised by staged withdrawal of therapy.18 symptomatic, non-atopic asthmatics were randomised (1:1) to receive omalizumab or identical placebo treatment in addition to existing therapy for 20 weeks. Bronchial biopsies were collected before and after 12-14 weeks of treatment, then the patients destabilised by substantial, supervised reduction of their regular therapy. Primary outcome measures were changes in bronchial mucosal IgE + cells at 12-14 weeks, prior to regular therapy reduction, and changes in lung function (forced expiratory volume in 1 s) after destabilisation at 20 weeks. Quality of life was also monitored.Omalizumab but not placebo therapy significantly reduced median total bronchial mucosal IgE + cells (pomalizumab treated patients, with significant differences in absolute (p=0.04) and % predicted forced expiratory volume in 1 s (p=0.015).Omalizumab therapy of non-atopic asthmatics reduces bronchial mucosal IgE + mast cells and improves lung function despite withdrawal of conventional therapy. Copyright ©ERS 2016.

  17. Targeting IgE in Severe Atopic Dermatitis with a Combination of Immunoadsorption and Omalizumab

    DEFF Research Database (Denmark)

    Zink, Alexander; Gensbaur, Anna; Zirbs, Michael

    2015-01-01

    immunoadsorption and anti-IgE antibody omalizumab in 10 patients with severe, therapy-refractory AD. IgE levels decreased after immunoadsorption and decreased continuously in all patients during anti-IgE therapy. The reverse trend was observed during 6 months follow-up without treatment. In parallel...

  18. Polyphenol-Rich Pomegranate Juice Reduces IgE Binding to Cashew Nut Allergens

    Science.gov (United States)

    Cashew nut allergy is mediated by IgE binding to seed-storage proteins including Ana o 1, 2, and 3. Cashew nuts commonly cause severe reactions and only small amounts are needed. Polyphenol rich juices and polyphenol compounds have been demonstrated to complex with peanut allergens. The interacti...

  19. Poor biologic activity of cross-reactive IgE directed to carbohydrate determinants of glycoproteins

    NARCIS (Netherlands)

    van der Veen, M. J.; van Ree, R.; Aalberse, R. C.; Akkerdaas, J.; Koppelman, S. J.; Jansen, H. M.; van der Zee, J. S.

    1997-01-01

    BACKGROUND: In our outpatient population, approximately one third of patients sensitized to grass pollen were found to have significant serum levels of anti-peanut IgE in the RAST, without positive peanut skin prick test (SPT) response and without peanut-related allergic symptoms. It was suggested

  20. Infusion reactions during infliximab treatment are not associated with IgE anti-infliximab antibodies

    NARCIS (Netherlands)

    van Schie, Karin A.; Ooijevaar-de Heer, Pleuni; Kruithof, Simone; Plasencia, Chamaida; Jurado, Teresa; Pascual Salcedo, Dora; Brandse, Johannan F.; D'Haens, Geert Ram; Wolbink, Gerrit Jan; Rispens, Theo

    2017-01-01

    Controversy exists on the role of IgE antidrug antibodies (IgE-ADA) in infusion reactions (IR) on infliximab treatment, partly due to the lack of a positive control used for assay validation. We sought to (1) develop a robust assay to measure IgE-ADA, including a positive control, (2) determine the

  1. Screening for hen's egg and chicken meat specific IgE antibodies in ...

    African Journals Online (AJOL)

    Abstract. Background: Allergy to hen's egg and meat contributes significantly to the manifestations of food allergy all over the world. Objectives: This study was performed to assess the presence of hen's egg and meat specific IgE antibodies among patients investigated for various allergic disorders. Methods. This is a ...

  2. Influencia de la diabetes mellitus sobre la evolución del paciente con enfermedad cerebrovascular y nutrición enteral por sonda

    OpenAIRE

    Coto García, Cristina

    2017-01-01

    La Diabetes Mellitus juega un importante papel tanto en la patogenia como en el pronóstico de la enfermedad cerebrovascular. El ictus y las complicaciones que este conlleva conforman una entidad de elevada morbimortalidad. El soporte nutricional constituye un pilar importante a la hora tratar a los pacientes que han sufrido un ictus. Valorar si existe diferencia en estancia media hospitalaria, éxitus y complicaciones relacionadas con la nutrición entre aquellos pacientes con diabetes melli...

  3. Relación entre geohelmintiasis intestinales y variables químicas, hematológicas e IgE, en una comunidad yukpa del estado Zulia, Venezuela

    OpenAIRE

    Rivero de Rodríguez, Zulbey; Churio, Osmaly; Bracho Mora, Angela; Calchi La Corte, Marinella; Acurero, Ellen; Villalobos, Rafael

    2012-01-01

    Para relacionar helmintiasis intestinales y parámetros hematológicos, bioquímicos y serológicos, se estudiaron 37 individuos yukpas del municipio Perijá, estado Zulia, durante el año 2009. Las muestras de heces se evaluaron mediante el concentrado con formol-éter, Kato-Katz y cultivo de Harada-Mori. Los parámetros hematológicos, las proteínas totales, albúminas, globulinas y relación A/G, fueron determinados por autoanalizadores hematológicos y bioquímicos. La IgE fue determinada por ELISA. 7...

  4. Tracing the Origins of IgE, Mast Cells, and Allergies by Studies of Wild Animals.

    Science.gov (United States)

    Hellman, Lars Torkel; Akula, Srinivas; Thorpe, Michael; Fu, Zhirong

    2017-01-01

    In most industrialized countries, allergies have increased in frequency quite dramatically during the past 50 years. Estimates show that 20-30% of the populations are affected. Allergies have thereby become one of the major medical challenges of the twenty-first century. Despite several theories including the hygiene hypothesis, there are still very few solid clues concerning the causes of this increase. To trace the origins of allergies, we have studied cells and molecules of importance for the development of IgE-mediated allergies, including the repertoire of immunoglobulin genes. These studies have shown that IgE and IgG most likely appeared by a gene duplication of IgY in an early mammal, possibly 220-300 million years ago. Receptors specific for IgE and IgG subsequently appeared in parallel with the increase in Ig isotypes from a subfamily of the recently identified Fc receptor-like molecules. Circulating IgE levels are generally very low in humans and laboratory rodents. However, when dogs and Scandinavian wolfs were analyzed, IgE levels were found to be 100-200 times higher compared to humans, indicating a generally much more active IgE synthesis in free-living animals, most likely connected to intestinal parasite infections. One of the major effector molecules released upon IgE-mediated activation by mast cells are serine proteases. These proteases, which belong to the large family of hematopoietic serine proteases, are extremely abundant and can account for up to 35% of the total cellular protein. Recent studies show that several of these enzymes, including the chymases and tryptases, are old. Ancestors for these enzymes were most likely present in an early mammal more than 200 million years ago before the separation of the three extant mammalian lineages; monotremes, marsupials, and placental mammals. The aim is now to continue these studies of mast cell biology and IgE to obtain additional clues to their evolutionary conserved functions. A focus

  5. Tracing the Origins of IgE, Mast Cells, and Allergies by Studies of Wild Animals

    Directory of Open Access Journals (Sweden)

    Lars Torkel Hellman

    2017-12-01

    Full Text Available In most industrialized countries, allergies have increased in frequency quite dramatically during the past 50 years. Estimates show that 20–30% of the populations are affected. Allergies have thereby become one of the major medical challenges of the twenty-first century. Despite several theories including the hygiene hypothesis, there are still very few solid clues concerning the causes of this increase. To trace the origins of allergies, we have studied cells and molecules of importance for the development of IgE-mediated allergies, including the repertoire of immunoglobulin genes. These studies have shown that IgE and IgG most likely appeared by a gene duplication of IgY in an early mammal, possibly 220–300 million years ago. Receptors specific for IgE and IgG subsequently appeared in parallel with the increase in Ig isotypes from a subfamily of the recently identified Fc receptor-like molecules. Circulating IgE levels are generally very low in humans and laboratory rodents. However, when dogs and Scandinavian wolfs were analyzed, IgE levels were found to be 100–200 times higher compared to humans, indicating a generally much more active IgE synthesis in free-living animals, most likely connected to intestinal parasite infections. One of the major effector molecules released upon IgE-mediated activation by mast cells are serine proteases. These proteases, which belong to the large family of hematopoietic serine proteases, are extremely abundant and can account for up to 35% of the total cellular protein. Recent studies show that several of these enzymes, including the chymases and tryptases, are old. Ancestors for these enzymes were most likely present in an early mammal more than 200 million years ago before the separation of the three extant mammalian lineages; monotremes, marsupials, and placental mammals. The aim is now to continue these studies of mast cell biology and IgE to obtain additional clues to their evolutionary conserved

  6. Potenciales evocados somatosensitivos en una poblacion cronicamente intoxicada con plomo

    Directory of Open Access Journals (Sweden)

    O. M. Genovese

    1990-03-01

    Full Text Available Once pacientes con cifras elevadas de plombemia y disminuidas de delta aminolevulínico dehidrasa (ALA D, fueron estudiados mediante la realización de potenciales evocados somatosensitivos obtenidos por estimulación del nervio mediano, midiendo el ingreso medular, el arribo cortical v el tiempo de conducción central (TCC. Los datos fueron comparados con controles. Se correlacionaron con las deterniinaciones bioquímicas y con el tiempo de exposición al tóxico. Por encima dei limite superior control, se encontro el ingreso medular en tres pacientes, el arribo cortical en cuatro y el TCC en tres. Los valores de N13,N20 y TCC no mostraron correlación con los parâmetros bioquímicos ni con el tiempo de exposición al tóxico. Estos hallazgos sugieren que, si bien la neuropatía periférica condiciona el retraso dei ingreso medular y del arribo cortical, es probable que exista también compromiso de la mielina central en algunos de los pacientes crónicamente expuestos.

  7. Interpretación de la prueba de tuberculina en niños y vacunados con BCG

    OpenAIRE

    Piñeiro Pérez, Roi

    2012-01-01

    Revisados 20 años de literatura médica, el debate sobre la conveniencia de la vacunación con Bacilo de Calmette-Guérin (BCG) continúa abierto. La OMS se muestra a favor de su utilización en países con endemia elevada de Tuberculosis (TB), pero no se ha esclarecido el beneficio ni las indicaciones de la inmunización en poblaciones con endemia intermedia o baja, y existen numerosos expertos que se muestran contrarios a su administración en cualquier caso. Existen muchas publicaciones sobre el ...

  8. Immunization with Hypoallergens of shrimp allergen tropomyosin inhibits shrimp tropomyosin specific IgE reactivity.

    Directory of Open Access Journals (Sweden)

    Christine Y Y Wai

    Full Text Available Designer proteins deprived of its IgE-binding reactivity are being sought as a regimen for allergen-specific immunotherapy. Although shrimp tropomyosin (Met e 1 has long been identified as the major shellfish allergen, no immunotherapy is currently available. In this study, we aim at identifying the Met e 1 IgE epitopes for construction of hypoallergens and to determine the IgE inhibitory capacity of the hypoallergens. IgE-binding epitopes were defined by three online computational models, ELISA and dot-blot using sera from shrimp allergy patients. Based on the epitope data, two hypoallergenic derivatives were constructed by site-directed mutagenesis (MEM49 and epitope deletion (MED171. Nine regions on Met e 1 were defined as the major IgE-binding epitopes. Both hypoallergens MEM49 and MED171 showed marked reduction in their in vitro reactivity towards IgE from shrimp allergy patients and Met e 1-sensitized mice, as well as considerable decrease in induction of mast cell degranulation as demonstrated in passive cutaneous anaphylaxis assay. Both hypoallergens were able to induce Met e 1-recognizing IgG antibodies in mice, specifically IgG2a antibodies, that strongly inhibited IgE from shrimp allergy subjects and Met e 1-sensitized mice from binding to Met e 1. These results indicate that the two designer hypoallergenic molecules MEM49 and MED171 exhibit desirable preclinical characteristics, including marked reduction in IgE reactivity and allergenicity, as well as ability to induce blocking IgG antibodies. This approach therefore offers promises for development of immunotherapeutic regimen for shrimp tropomyosin allergy.

  9. IgE antibodies of fish allergic patients cross-react with frog parvalbumin.

    Science.gov (United States)

    Hilger, C; Thill, L; Grigioni, F; Lehners, C; Falagiani, P; Ferrara, A; Romano, C; Stevens, W; Hentges, F

    2004-06-01

    The major allergens in fish are parvalbumins. Important immunoglobulin (Ig)E cross-recognition of parvalbumins from different fish species has been shown. Recently frog parvalbumin alpha has been found to be responsible for a case of IgE-mediated anaphylaxis triggered by the ingestion of frog meat. The aim of this study was to investigate whether IgE antibodies of fish allergic persons cross-react with frog parvalbumin and to appreciate its clinical relevance. The sera of 15 fish allergic patients and one fish and frog allergic patient were tested by IgE-immunoblotting against frog muscle extract. Sera were tested against recombinant parvalbumin alpha and beta from Rana esculenta. Skin prick tests were performed in selected patients with recombinant frog parvalbumin. Ca(2+) depletion experiments and inhibition studies with purified cod and frog recombinant parvalbumin were done to characterize the cross-reactive pattern. Fourteen of the sera tested had IgE antibodies recognizing low molecular weight components in frog muscle extract. Calcium depletion experiments or inhibition of patient sera with purified cod parvalbumin led to a significant or complete decrease in IgE binding. When tested against recombinant parvalbumins, three of 13 sera reacted with alpha parvalbumin and 11 of 12 reacted with beta parvalbumin from R. esculenta. Skin prick tests performed with recombinant frog parvalbumin were positive in fish allergic patients. Inhibition studies showed that a fish and frog allergic patient was primarily sensitized to fish parvalbumin. Cod parvalbumin, a major cross-reactive allergen among different fish species, shares IgE binding epitopes with frog parvalbumin. This in vitro cross-reactivity seems to be also clinically relevant. Parvalbumins probably represent a new family of cross-reactive allergens.

  10. IgE Sensitization Profiles Differ between Adult Patients with Severe and Moderate Atopic Dermatitis.

    Science.gov (United States)

    Mittermann, Irene; Wikberg, Gustav; Johansson, Catharina; Lupinek, Christian; Lundeberg, Lena; Crameri, Reto; Valenta, Rudolf; Scheynius, Annika

    2016-01-01

    Atopic dermatitis (AD) is a complex chronic inflammatory disease where allergens can act as specific triggering factors. To characterize the specificities of IgE-reactivity in patients with AD to a broad panel of exogenous allergens including microbial and human antigens. Adult patients with AD were grouped according to the SCORAD index, into severe (n = 53) and moderate AD (n = 126). As controls 43 patients were included with seborrhoeic eczema and 97 individuals without history of allergy or skin diseases. Specific IgE reactivity was assessed in plasma using Phadiatop®, ImmunoCap™, micro-arrayed allergens, dot-blotted recombinant Malassezia sympodialis allergens, and immune-blotted microbial and human proteins. IgE reactivity was detected in 92% of patients with severe and 83% of patients with moderate AD. Sensitization to cat allergens occurred most frequently, followed by sensitization to birch pollen, grass pollen, and to the skin commensal yeast M. sympodialis. Patients with severe AD showed a significantly higher frequency of IgE reactivity to allergens like cat (rFel d 1) and house dust mite (rDer p 4 and 10), to Staphylococcus aureus, M. sympodialis, and to human antigens. In contrast, there were no significant differences in the frequencies of IgE reactivity to the grass pollen allergens rPhl p 1, 2, 5b, and 6 between the two AD groups. Furthermore the IgE reactivity profile of patients with severe AD was more spread towards several different allergen molecules as compared to patients with moderate AD. We have revealed a hitherto unknown difference regarding the molecular sensitization profile in patients with severe and moderate AD. Molecular profiling towards allergen components may provide a basis for future investigations aiming to explore the environmental, genetic and epigenetic factors which could be responsible for the different appearance and severity of disease phenotypes in AD.

  11. Epigenome-wide DNA methylation study of IgE concentration in relation to self-reported allergies.

    Science.gov (United States)

    Ek, Weronica E; Ahsan, Muhammad; Rask-Andersen, Mathias; Liang, Liming; Moffatt, Miriam F; Gyllensten, Ulf; Johansson, Åsa

    2017-04-01

    Epigenetic mechanisms are critical for normal immune development and epigenetic alterations might therefore be possible contributors to immune diseases. To investigate if DNA methylation in whole blood is associated with total and allergen-specific IgE levels. We performed an epigenome-wide association study to investigate the association between DNA methylation and IgE level, allergen-specific IgE and self-reported immune diseases and allergies in 728 individuals. We identified and replicated 15 CpG sites associated with IgE, mapping to biologically relevant genes, including ACOT7, ILR5A, KCNH2, PRG2 and EPX. A total of 331 loci were associated with allergen-specific IgE, but none of these CpG sites were associated with self-reported allergies and immune diseases. This study shows that IgE levels are associated with DNA methylation levels at numerous CpG sites, which might provide new leads for investigating the links between IgE and allergic inflammation.

  12. IgE binding to peanut allergens is inhibited by combined D-aspartic and D-glutamic acids.

    Science.gov (United States)

    Chung, Si-Yin; Reed, Shawndrika

    2015-01-01

    The objective of this study was to determine if D-amino acids (D-aas) bind and inhibit immunoglobulin E (IgE) binding to peanut allergens. D-aas such as D-Asp (aspartic acid), D-Glu (glutamic acid), combined D-[Asp/Glu] and others were each prepared in a cocktail of 9 other D-aas, along with L-amino acids (L-aas) and controls. Each sample was mixed with a pooled plasma from peanut-allergic donors, and tested by ELISA (enzyme-linked immunosorbent assay) and Western blots for IgE binding to peanut allergens. Results showed that D-[Asp/Glu] (4 mg/ml) inhibited IgE binding (75%) while D-Glu, D-Asp and other D-aas had no inhibitory effect. A higher inhibition was seen with D-[Asp/Glu] than with L-[Asp/Glu]. We concluded that IgE was specific for D-[Asp/Glu], not D-Asp or D-Glu, and that D-[Asp/Glu] was more reactive than was L-[Asp/Glu] in IgE inhibition. The finding indicates that D-[Asp/Glu] may have the potential for removing IgE or reducing IgE binding to peanut allergens in vitro. Published by Elsevier Ltd.

  13. Natural history of perceived food hypersensitivity and IgE sensitisation to food allergens in a cohort of adults.

    Science.gov (United States)

    Patelis, Antonios; Gunnbjörnsdottir, Maria; Borres, Magnus P; Burney, Peter; Gislason, Thorarinn; Torén, Kjell; Forsberg, Bertil; Alving, Kjell; Malinovschi, Andrei; Janson, Christer

    2014-01-01

    No longitudinal studies exist on the natural history of food hypersensitivity and IgE sensitisation to food allergens in adults. To examine the natural history of food hypersensitivity, the natural history of IgE sensitisation to food allergens and to investigate the risk factors for new onset food hypersensitivity. Food hypersensitivity was questionnaire-assessed in 2307 individuals (aged 20-45 years) from Iceland and Sweden during the European Community Respiratory Health Survey both at baseline and follow-up 9 years later. IgE food and aeroallergen sensitisation were assessed in a subgroup of these individuals (n = 807). Values of 0.35 kU/L and above were regarded as positive sensitisation. Food hypersensitivity was reported by 21% of the subjects and this proportion remained unchanged at follow-up (p = 0.58). Fruits, nuts and vegetables were the three most common causes of food hypersensitivity, with a similar prevalence at baseline and follow-up. The prevalence IgE sensitisation to food allergens decreased in general by 56% (pfood hypersensitivity. The prevalence of food hypersensitivity remained unchanged while the prevalence of IgE sensitisation to food allergens decreased in adults over a 9-year follow-up period. The decrease in prevalence of IgE sensitisation to food allergens was considerably larger than the change in prevalence of IgE sensitisation to aeroallergens.

  14. Differences in total and allergen specific IgE during pregnancy compared with 1 month and 1 year post partum.

    Science.gov (United States)

    Perry, Lee M; Ownby, Dennis R; Wegienka, Ganesa R; Peterson, Edward L; Woodcroft, Kimberly J; Joseph, Christine L; Johnson, Christine C

    2009-10-01

    Pregnancy alters the function of many body systems, including the immune system. However, little is known regarding the effect of pregnancy on maternal IgE levels or atopy. To determine whether pregnancy consistently influences serum levels of total or allergen specific IgE. Blood samples were obtained from 764 women during the third trimester of pregnancy and 1 month post partum. A third sample was obtained from 106 of these women 1 year post partum. Samples were analyzed for total and specific IgE to 8 regionally common allergens using a commercially available system. Sensitization was defined as an allergen specific IgE level of 0.35 kU of allergen per liter or higher to any allergen. Total IgE increased significantly post partum, both at 1 month (40.36 vs 35.37 IU/mL intrapartum; P = .001) and at 1 year (44.97 vs 37.00 IU/mL intrapartum; P = .005). Allergen specific IgE decreased significantly at 1 month for cat, dog, ragweed, timothy grass, and egg (P = .001 to P = .02) but not for dust mite, cockroach, or Alternaria (P = .15 to P = .90). Similar patterns of change in total and specific IgE were seen at 1 year. However, on average, only 3.5% of participants changed sensitization status to the individual allergens studied during the 1 year of observation. Compared with intrapartum levels, total IgE levels increased significantly at 1 month and 1 year post partum. Conversely, at the same time points, IgE levels specific for common allergens significantly declined to most but not all allergens. Few women changed their sensitization status over 1 year.

  15. Effect of filgrastim (recombinant human granulocyte colony stimulating factor) on IgE responses in human asthma: a case study.

    Science.gov (United States)

    Smith-Norowitz, Tamar A; Joks, Rauno; Norowitz, Kevin B; Chice, Seto; Durkin, Helen G; Bluth, Martin H

    2013-10-01

    The role of peripheral blood progenitor cell mobilization on Immunoglobulin E (IgE) responses has not been studied. Distributions of blood lymphocytes (CD4+, CD8+, CD8+CD60+, CD19+, CD23+, CD16/56+, CD25, CD45RA+, CD45RO+, CD34+), and levels of serum immunoglobulins (IgM, IgG, IgA, IgE) were studied in an allergic asthmatic serum IgE+ (181IU/mL) adult (m/45 y/o) donor undergoing routine stem cell mobilization protocol (American Society of Hematology) before (day-30), during (day 4), and after (1 wk post last dose) filgrastim (subcutaneous, 480 mcg, 2qd) treatment (flow cytometry, nephelometry, UniCAP Total IgE Fluoro enzyme immunoassay). On day 4 of filgrastim treatment, numbers of CD8+CD60+T cells and CD23+ blood cells dramatically increased (98% and 240% respectively) compared with pre treatment. In contrast on day 4 of treatment, serum IgE levels decreased (>50%) compared with pre treatment. CD8+CD60+T cells and CD23+ blood cells and serum IgE levels approached pre-treatment levels at 1 week post treatment. Filgrastim treatment transiently increases numbers of CD8+CD60+T and CD23+ expressing cells, which are known to regulate human IgE responses, while also transiently suppressing ongoing IgE responses. These results suggest that filgrastim affects IgE related responses, and may be useful in modulating allergic responses. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. Dosis elevada en el dosímetro personal de una auxiliar de enfermería en Electrofisiología A high dose in the personal dosage meter of nursing assistant in Electrophysiology field

    Directory of Open Access Journals (Sweden)

    Raquel Barquero Sanz

    2012-03-01

    Full Text Available En una sala de Electrofisiología del Servicio de Cardiología en donde se efectúan procedimientos guiados con rayos X de ablación con radiofrecuencia y de implantes de desfibrilador biventricular, se registraron dosis mensuales anormalmente elevadas en el dosímetro personal de solapa de una auxiliar de enfermería que trabaja en la misma, con valores de dosis equivalente personal, Hp(10, de 125 mSv y de dosis equivalente personal superficial, Hp(0.07 de 128.1 mSv. Ante la ansiedad producida en la trabajadora al conocer las lecturas de su dosímetro, y dado que no se dispone de precedentes investigados en la instalación, se ha realizado un estudio para dilucidar si la dosis registrada ha podido ser recibida por la trabajadora o si solo ha sido recibida por el dosímetro. Se hacen medidas con un monitor ambiental en condiciones simuladas de los procedimientos, para estimar las dosis reales que pueda recibir el personal de la sala. Se comparan estos valores con los del rendimiento del equipo de RX en haz directo medidos durante la revisión anual de control de calidad del mismo, concluyéndose que ha sido el dosímetro, y no la trabajadora, el que se ha expuesto a radiación directa, siendo la situación más probable la de que dicho dosímetro cayó sobre el tubo de RX, normalmente orientado hacia el techo, un tiempo del orden de 1 minuto. Paralelamente a estas estimaciones dosimétricas, el Servicio de Prevención de Riesgos Laborales realizó la vigilancia de la salud, concluyendo que la trabajadora no tenía ningún indicio de haber recibido dosis de la magnitud registrada por su dosímetro.In a room of electrophysiology from the cardiology service where procedures are made guided with X-Rays of ablation with radiofrequency and from implants ventricular defibrillation; there were registered monthly doses usually high in the same personal dosimeter flap of a nursing assistant who works there with values of equivalent personal doses, Hp (10

  17. The Subcritical Assembly for High-Temperature Use; Assemblage Sous-Critique Pour Emploi a Haute Temperature; K voprosu o podkriticheskoj sborke dlya ispol'zovaniya pri vysokikh temperaturakh; Conjunto Subcritico para Temperatura Elevada

    Energy Technology Data Exchange (ETDEWEB)

    Sakurai, Y.; Sekiya, T.; Suita, T. [Osaka University (Japan); Hishida, H.; Hamada, H.; Nagashima, K. [Sumitomo Atomic Energy Industries Group (Japan)

    1964-04-15

    et pour les detecteurs. Il met egalement au point une methode particuliere pour mesurer la densite du flux de neutrons a haute temperature. Enfin, il a l'intention d'etudier l'influence des hautes temperatures sur les parametres de reacteur. (author) [Spanish] El autor estudio un conjunto subcritico del tipo alimentado por uranio natural y moderado por grafito. Este conjunto se caracteriza por la existencia de una zona de elevada temperatura que se puede crear en un cuerpo de menor temperatura y que permite obtener informaciones aplicables a un futuro reactor de temperatura elevada refrigerado con gas, asi como datos sobre la conversion directa de la energia nuclear en electricidad en un medio gaseoso a temperatura elevada. Ademas, el conjunto se utiliza para el adiestramiento de los estudiantes universitarios. El conjunto esta constituido por barras cuadradas de grafito de 10 cm de lado apiladas hasta formar un volumen de 2 x 2 x 3 m. La zona de alta temperatura, que tiene 1 m3, se calienta por efecto Joule y puede mantenerse a temperaturas hasta de 2000 Degree-Sign C. En la zona de baja temperatura se usa combustible de pastillas de UO{sub 2}, mientras que para la zona de temperatura elevada, se han preparado pastillas de UC{sub 2}. El combustible esta revestido de vainas de grafito, de seccion cuadrada. Las fuentes de Am-Be, de 5 c se instalan debajo del pedestal ubicado en la base del conjunto, mientras que el flujo neutronico pulsante se inyecta en un punto arbitrario. El blanco, situado en el extremo del tubo de extension del acelerador, es bombardeado por el haz de deuterones. Las barras de conexion para el calentamiento por efecto Joule se enfrian con agua y la superficie total de separacion de la zona de alta temperatura se enfria con helio. Este circuito de gas refrigerante esta diseflado de manera que pueda servir para investigar la conversion directa de la energia nuclear en electricidad. El conjunto esta provisto de dispositivos de control y medicion

  18. Insect Sting Reactions and Specific IgE to Venom and Major Allergens in a General Population

    DEFF Research Database (Denmark)

    Mosbech, H; Tang, L; Linneberg, A

    2016-01-01

    BACKGROUND: Insect sting reactions are frequently reported, but population studies documenting the frequency and the relation to IgE-sensitization and serum tryptase are scarce. METHODS: Questionnaire data and results from measurements of specific IgE against venom, major allergens and cross...... or wasp. IgE to CCDs occurred in only 0.7%, but 80% of these were DS. Finally, 36% with IgE to CCDs had had symptoms, mostly local. Serum tryptase was not associated with a history of sting reactions. CONCLUSIONS: In a temperate climate, self-reported insect sting reactions and sensitization to venom...

  19. Increase of lymphocytes with Fc receptors for IgE in patients with allergic rhinitis during the grass pollen season.

    OpenAIRE

    Spiegelberg, H L; Simon, R A

    1981-01-01

    Peripheral blood lymphocytes from 10 nonallergic donors and 7 patients suffering from seasonal allergic rhinitis and receiving desensitization therapy were analyzed by rosette assays for Fc receptors for IgE (Fc epsilon R) and IgG (Fc gamma R) before, during and after the grass pollen season. Six of seven patients had moderately elevated IgE levels (330 +/- 268 IU/ml), all had high titers of skin sensitizing antibodies to grass pollens and serum IgE antibodies as measured by radio-allergosorb...

  20. Hyper IgE in Childhood Eczema and Risk of Asthma in Chinese Children

    Directory of Open Access Journals (Sweden)

    Chantel Ng

    2016-06-01

    Full Text Available Background: Atopic eczema is a common childhood disease associated with high IgE and eosinophilia. We characterized the clinical features associated with hyper-IgE (defined as IgE > 2000 IU/L in eczema. Methods: Nottingham Eczema Severity Score (NESS, family and personal history of atopy, skin prick test (SPT for common food and aeroallergens, highest serum IgE ever and eosinophil counts were evaluated in 330 children eczema patients. Childhood-NESS (NESS performed at <10 years of age and adolescent-NESS (NESS performed at >10 years of age were further analyzed. Results: IgE correlated with NESS (spearman coefficient 0.35, p < 0.001 and eosinophil percentage (spearman coefficient 0.56, p = 0.001. Compared with IgE ≤ 2000IU/L (n = 167, patients with hyper-IgE (n = 163 were associated with male gender (p = 0.002; paternal atopy (p = 0.026; personal history of atopic rhinitis (p = 0.016; asthma (p < 0.001; dietary avoidance (p < 0.001; use of wet wrap (p < 0.001; traditional Chinese medicine use (TCM, p < 0.001; immunomodulant use (azathioprine or cyclosporine, p < 0.001; skin prick sensitization by dust mites (p < 0.001, cats (p = 0.012, dogs (p = 0.018, food (p = 0.002; eosinophilia (p < 0.001; more severe disease during childhood (p < 0.0001 and during adolescence (p < 0.0001, but not onset age of eczema or maternal atopy. Logistic regression showed that hyper-IgE was associated with personal history of asthma (exp(B = 5.12, p = 0.002 and eczema severity during childhood and adolescence (p < 0.001. For patients <10 years of age, dust mite sensitization (p = 0.008 was associated with hyper-IgE. For patients >10years of age, food allergen sensitization was associated with hyper-IgE (p = 0.008. Conclusions: Hyper-IgE is independently associated with asthma, more severe atopy and more severe eczema during childhood and adolescence. IgE > 2000 IU/L may be a tool to aid prognostication of this chronic relapsing dermatologic disease and its

  1. Isotopic and enzymatic IgE assays in non-allergic subjects

    International Nuclear Information System (INIS)

    Stein, R.; Evans, S.; Milner, R.; Rand, C.; Dolovich, J.

    1983-01-01

    In order to establish normal values in an adult population for serum IgE concentration, sera were obtaned from 446 ambulatory Canadian caucasian subjects with negative allergy histories. A standard isotopic procedure, the Phadebas paper radioimmunosorbent test (PRIST), was compared with the new enzymatic counterpart, the Phadezyme PRIST. By the isotopic method, serum IgE concentrations in women and men were comparable from one age group to another with no age-related trend in the seven age groups examined (15-20, 21-30, 31-40, 41-50, 51-60, 61-70, above 70). The median and 95th percentile units (U)/ml respectively were 17.5 and 145 for 224 women and 25.5 and 275 for 222 men. Mean values +- 1 SD for women were 43 +- 102 and for men, 58 +- 137. Levels were significantly higher in men as a group. Sera with IgE concentrations above 100 U and a sampling of additional sera were tested for specific IgE antibodies to 13 common allergens by the radioallergosorbent test (RAST). After exclusion of RAST-positive sera, the mean U/ml values +- ISD were 22 +- 29 for 204 women and 37 +- 54 for 196 men. Geometric mean U/ml values for these sera were 14.6 for women and 22.3 for men and the median and 95th percentile U/ml respectively for the women were 15 and 66, for the men, 24 and 135. These 95th percentile values are considered the upper limits of normal in this population. The RAST identifiction of antibodies to allergens to which sensitization was demonstrated provided a potential explanation for serum IgE concentrations above 100 U/ml in less than 30% of the sera in this population with negative allergy histories. The isotopic method and the counterpart enzymatic method (Phadezyme PRIST) were highly comparable; the correlation coefficient (r) for all the sera was +- 0.93(P<0.001). IgE levels were significantly higher in male smokers than non-smokers by both methods but these differences were not significant in women. (author)

  2. Prevalence of IgE antibodies to grain and grain dust in grain elevator workers

    Energy Technology Data Exchange (ETDEWEB)

    Lewis, D.M.; Romeo, P.A.; Olenchock, S.A.

    1986-04-01

    IgE-mediated allergic reactions have been postulated to contribute to respiratory reactions seen in workers exposed to grain dusts. In an attempt better to define the prevalence of IgE antibodies in workers exposed to grain dusts, we performed the radioallergosorbent test (RAST) on worker sera using both commercial allergens prepared from grain and worksite allergens prepared from grain dust samples collected at the worksite. We found that the two types of reagents identified different populations with respect to the specificity of IgE antibodies present. The RAST assay performed using worksite allergens correlated well with skin test procedures. These results may allow us to gain better understanding of allergy associated with grain dust exposure, and document the utility of the RAST assay in assessment of occupational allergies.

  3. Prevalence of IgE antibodies to grain and grain dust in grain elevator workers.

    Science.gov (United States)

    Lewis, D M; Romeo, P A; Olenchock, S A

    1986-01-01

    IgE-mediated allergic reactions have been postulated to contribute to respiratory reactions seen in workers exposed to grain dusts. In an attempt better to define the prevalence of IgE antibodies in workers exposed to grain dusts, we performed the radioallergosorbent test (RAST) on worker sera using both commercial allergens prepared from grain and worksite allergens prepared from grain dust samples collected at the worksite. We found that the two types of reagents identified different populations with respect to the specificity of IgE antibodies present. The RAST assay performed using worksite allergens correlated well with skin test procedures. These results may allow us to gain better understanding of allergy associated with grain dust exposure, and document the utility of the RAST assay in assessment of occupational allergies. PMID:3709478

  4. A new framework for the interpretation of IgE sensitization tests

    DEFF Research Database (Denmark)

    Roberts, G; Ollert, M; Aalberse, R.

    2016-01-01

    tests to make a definitive diagnosis; these are often expensive and potentially associated with severe reactions. The likelihood of clinical allergy can be semi-quantified from an IgE sensitization test results. This relationship varies though according to the patients' age, ethnicity, nature...... of the putative allergic reaction and coexisting clinical diseases such as eczema. The likelihood of clinical allergy can be more precisely estimated from an IgE sensitization test result, by taking into account the patient's presenting features (pretest probability). The presence of each of these patient...... pretest probabilities for diverse setting, regions and allergens. Also, cofactors, such as exercise, may be necessary for exposure to an allergen to result in an allergic reaction in specific IgE-positive patients. The diagnosis of IgE-mediated allergy is now being aided by the introduction of allergen...

  5. Prevalence of IgE antibodies to grain and grain dust in grain elevator workers

    International Nuclear Information System (INIS)

    Lewis, D.M.; Romeo, P.A.; Olenchock, S.A.

    1986-01-01

    IgE-mediated allergic reactions have been postulated to contribute to respiratory reactions seen in workers exposed to grain dusts. In an attempt better to define the prevalence of IgE antibodies in workers exposed to grain dusts, we performed the radioallergosorbent test (RAST) on worker sera using both commercial allergens prepared from grain and worksite allergens prepared from grain dust samples collected at the worksite. We found that the two types of reagents identified different populations with respect to the specificity of IgE antibodies present. The RAST assay performed using worksite allergens correlated well with skin test procedures. These results may allow us to gain better understanding of allergy associated with grain dust exposure, and document the utility of the RAST assay in assessment of occupational allergies

  6. Changes of plasma ET and serum IgE levels in children with asthma before and after allergen immunotherapy (AIT)

    International Nuclear Information System (INIS)

    Dai Yunhai; Cheng Guanghua; Xie Jiazhen

    2007-01-01

    Objective: To investigate the relationship between the changes of plasma ET-1, serum IgE levels and allergen immunotherapy(AIT) in children with asthma. Methods: Plasma levels of ET-1 and serum levels of IgE were measured with radioimmunoassay in 46 children with asthma before and after allergen immunotherapy. Results: In 35 patients benefited from allergen immunotherapy, the plasma levels of ET-1 (59.1 ± 11.7pg/ml) and serum levels of IgE (11.1 ± 3.2IU/ml) were higher significantly than those in controls (P 0.05, respectively). Conclusion: These data suggested that the changes of ET-1 and IgE levels after AIT may play an important role in the effective treatment of asthma with AIT. (authors)

  7. Efficacy and safety of omalizumab in patients with chronic urticaria who exhibit IgE against thyroperoxidase

    DEFF Research Database (Denmark)

    Maurer, Marcus; Altrichter, Sabine; Bieber, Thomas

    2011-01-01

    BACKGROUND: A subgroup of patients with chronic spontaneous urticaria (CU) exhibits IgE antibodies directed against autoantigens, such as thyroperoxidase (TPO). We conducted this study to investigate whether such patients with CU with IgE against TPO benefit from treatment with omalizumab......, a humanized anti-IgE mAb licensed for the treatment of severe persistent allergic (IgE-mediated) asthma. OBJECTIVES: We sought to assess the efficacy of omalizumab treatment in patients with CU with IgE autoantibodies against TPO. METHODS: In this multicenter, randomized, double-blind, placebo......-controlled study patients with CU (male/female, 18-70 years of age) with IgE autoantibodies against TPO who had persistent symptoms (wheals and pruritus) despite standard antihistamine therapy were randomized to receive either omalizumab (75-375 mg, dose determined by using the approved asthma dosing table...

  8. 98 Specific IGE and IGG Binding to Allergoids of Phleum pratense

    OpenAIRE

    Cases, Barbara; Fernandez-Caldas, Enrique; Tudela, Jose Ignacio; Fernandez, Eva Abel; Sanchez-Garcia, Silvia; Ibañez, M. Dolores; Escudero, Carmelo; Casanovas, Miguel

    2012-01-01

    Background Allergoids were first used in the decades of the 60s and 70s of the last century as an effective treatment of allergic respiratory diseases. Allergoids can be modified with formaldehyde or glutaraldehyde. Modified allergens, or allergoids, decrease the risk of adverse reactions while administering higher allergen doses. The objective of this study was to analyse specific IgE and IgG binding to glutaraldehyde modified and non-modified allergen extracts of Phleum pratense. Methods Th...

  9. IgE Sensitization Profiles Differ between Adult Patients with Severe and Moderate Atopic Dermatitis.

    Directory of Open Access Journals (Sweden)

    Irene Mittermann

    Full Text Available Atopic dermatitis (AD is a complex chronic inflammatory disease where allergens can act as specific triggering factors.To characterize the specificities of IgE-reactivity in patients with AD to a broad panel of exogenous allergens including microbial and human antigens.Adult patients with AD were grouped according to the SCORAD index, into severe (n = 53 and moderate AD (n = 126. As controls 43 patients were included with seborrhoeic eczema and 97 individuals without history of allergy or skin diseases. Specific IgE reactivity was assessed in plasma using Phadiatop®, ImmunoCap™, micro-arrayed allergens, dot-blotted recombinant Malassezia sympodialis allergens, and immune-blotted microbial and human proteins.IgE reactivity was detected in 92% of patients with severe and 83% of patients with moderate AD. Sensitization to cat allergens occurred most frequently, followed by sensitization to birch pollen, grass pollen, and to the skin commensal yeast M. sympodialis. Patients with severe AD showed a significantly higher frequency of IgE reactivity to allergens like cat (rFel d 1 and house dust mite (rDer p 4 and 10, to Staphylococcus aureus, M. sympodialis, and to human antigens. In contrast, there were no significant differences in the frequencies of IgE reactivity to the grass pollen allergens rPhl p 1, 2, 5b, and 6 between the two AD groups. Furthermore the IgE reactivity profile of patients with severe AD was more spread towards several different allergen molecules as compared to patients with moderate AD.We have revealed a hitherto unknown difference regarding the molecular sensitization profile in patients with severe and moderate AD. Molecular profiling towards allergen components may provide a basis for future investigations aiming to explore the environmental, genetic and epigenetic factors which could be responsible for the different appearance and severity of disease phenotypes in AD.

  10. Relationship between total and specific IgE in patients with asthma from Siberia

    Czech Academy of Sciences Publication Activity Database

    Gusareva, Elena; Ogorodova, L.M.; Chernyak, L.M.; Lipoldová, Marie

    0091-6749, č. 121 (2008), s. 781-781 ISSN 0091-6749 R&D Projects: GA ČR GD310/03/H147; GA MŠk(CZ) LC06009 Grant - others:EC(XE) 05-1000004-7761 Institutional research plan: CEZ:AV0Z50520514 Keywords : total and specific IgE * asthma * Russian population Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 9.773, year: 2008

  11. Recombinant allergen-based IgE testing to distinguish bee and wasp allergy.

    Science.gov (United States)

    Mittermann, Irene; Zidarn, Mihaela; Silar, Mira; Markovic-Housley, Zora; Aberer, Werner; Korosec, Peter; Kosnik, Mitja; Valenta, Rudolf

    2010-06-01

    The identification of the disease-causing insect in venom allergy is often difficult. To establish recombinant allergen-based IgE tests to diagnose bee and yellow jacket wasp allergy. Sera from patients with bee and/or wasp allergy (n = 43) and patients with pollen allergy with false-positive IgE serology to venom extracts were tested for IgE reactivity in allergen extract-based tests or with purified allergens, including nonglycosylated Escherichia coli-expressed recombinant (r) Api m 1, rApi m 2, rVes v 5, and insect cell-expressed, glycosylated rApi m 2 as well as 2 natural plant glycoproteins (Phl p 4, bromelain). The patients with venom allergy could be diagnosed with a combination of E coli-expressed rApi m 1, rApi m 2, and rVes v 5 whereas patients with pollen allergy remained negative. For a group of 29 patients for whom the sensitizing venom could not be identified with natural allergen extracts, testing with nonglycosylated allergens allowed identification of the sensitizing venom. Recombinant nonglycosylated allergens also allowed definition of the sensitizing venom for those 14 patients who had reacted either with bee or wasp venom extracts. By IgE inhibition studies, it is shown that glycosylated Api m 2 contains carbohydrate epitopes that cross-react with natural Api m 1, Ves v 2, natural Phl p 4, and bromelain, thus identifying cross-reactive structures responsible for serologic false-positive test results or double-positivity to bee and wasp extracts. Nonglycosylated recombinant bee and wasp venom allergens allow the identification of patients with bee and wasp allergy and should facilitate accurate prescription of venom immunotherapy. Copyright (c) 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

  12. Aerobic Exercise Decreases Lung Inflammation by IgE Decrement in an OVA Mice Model.

    Science.gov (United States)

    Camargo Hizume-Kunzler, Deborah; Greiffo, Flavia R; Fortkamp, Bárbara; Ribeiro Freitas, Gabriel; Keller Nascimento, Juliana; Regina Bruggemann, Thayse; Melo Avila, Leonardo; Perini, Adenir; Bobinski, Franciane; Duarte Silva, Morgana; Rocha Lapa, Fernanda; Paula Vieira, Rodolfo; Vargas Horewicz, Verônica; Soares Dos Santos, Adair Roberto; Cattelan Bonorino, Kelly

    2017-06-01

    Aerobic exercise (AE) reduces lung function decline and risk of exacerbations in asthmatic patients. However, the inflammatory lung response involved in exercise during the sensitization remains unclear. Therefore, we evaluated the effects of exercise for 2 weeks in an experimental model of sensitization and single ovalbumin-challenge. Mice were divided into 4 groups: mice non-sensitized and not submitted to exercise (Sedentary, n=10); mice non-sensitized and submitted to exercise (Exercise, n=10); mice sensitized and exposed to ovalbumin (OVA, n=10); and mice sensitized, submitted to exercise and exposed to OVA (OVA+Exercise, n=10). 24 h after the OVA/saline exposure, we counted inflammatory cells from bronchoalveolar fluid (BALF), lung levels of total IgE, IL-4, IL-5, IL-10 and IL-1ra, measurements of OVA-specific IgG1 and IgE, and VEGF and NOS-2 expression via western blotting. AE reduced cell counts from BALF in the OVA group (p<0.05), total IgE, IL-4 and IL-5 lung levels and OVA-specific IgE and IgG1 titers (p<0.05). There was an increase of NOS-2 expression, IL-10 and IL-1ra lung levels in the OVA groups (p<0.05). Our results showed that AE attenuated the acute lung inflammation, suggesting immunomodulatory properties on the sensitization process in the early phases of antigen presentation in asthma. © Georg Thieme Verlag KG Stuttgart · New York.

  13. Respiratory infections in adults with atopic disease and IgE antibodies to common aeroallergens.

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    Aino Rantala

    Full Text Available BACKGROUND: Atopic diseases, including allergic rhinitis, allergic dermatitis and asthma, are common diseases with a prevalence of 30-40% worldwide and are thus of great global public health importance. Allergic inflammation may influence the immunity against infections, so atopic individuals could be susceptible to respiratory infections. No previous population-based study has addressed the relation between atopy and respiratory infections in adulthood. We assessed the relation between atopic disease, specific IgE antibodies and the occurrence of upper and lower respiratory infections in the past 12 months among working-aged adults. METHODS AND FINDINGS: A population-based cross-sectional study of 1008 atopic and non-atopic adults 21-63 years old was conducted. Information on atopic diseases, allergy tests and respiratory infections was collected by a questionnaire. Specific IgE antibodies to common aeroallergens were measured in serum. Adults with atopic disease had a significantly increased risk of lower respiratory tract infections (LRTI; including acute bronchitis and pneumonia with an adjusted risk ratio (RR 2.24 (95% confidence interval [CI] 1.43, 3.52 and upper respiratory tract infections (URTI; including common cold, sinusitis, tonsillitis, and otitis media with an adjusted RR 1.55 (1.14, 2.10. The risk of LRTIs increased with increasing level of specific IgE (linear trend P = 0.059. CONCLUSIONS: This study provides new evidence that working-aged adults with atopic disease experience significantly more LRTIs and URTIs than non-atopics. The occurrence of respiratory infections increased with increasing levels of specific IgE antibodies to common aeroallergens, showing a dose-response pattern with LRTIs. From the clinical point of view it is important to recognize that those with atopies are a risk group for respiratory infections, including more severe LRTIs.

  14. A study of the prevalence and clinical significance of venom-specific IgE.

    Science.gov (United States)

    Zora, J A; Swanson, M C; Yunginger, J W

    1988-01-01

    The prevalence of unrecognized Hymenoptera-venom sensitization, assessed by venom skin tests (VSTs) in adults with no history of adverse reactions to sting, has been as high as 12% in previous epidemiologic studies. To assess further the clinical importance of positive VSTs in such individuals, we skin tested 33 subjects stung in the field during the preceding 12 months without reaction, and 33 persons who denied being stung in the preceding 3 years. Among the recently stung group, 12/33 had at least one positive VST (greater than or equal to 2+) at 1.0 microgram/ml, whereas 5/33 had positive VST at 0.1 microgram/ml. In contrast, only 2/33 nonstung subjects had positive VST at 1.0 microgram/ml, and none were positive at 0.1 microgram/ml. To estimate, prospectively, the sensitization rate after insect stings, we studied a third group of 11 nonsensitive patients with negative skin tests to Hymenoptera. After a deliberate in-hospital honeybee sting, only 1/11 developed a persistently positive honeybee VST. From among the three groups, we then performed nine sting challenges in eight patients with positive VSTs, and all stings were tolerated without significant reaction. We also measured IgE antibodies to Hymenoptera venoms in random blood bank donors. During April to May, 2/216 sera contained elevated venom-specific IgE antibodies, whereas 14/201 sera collected from October to November contained elevated venom-specific IgE antibodies. We conclude that a small but appreciable portion of the population has venom-specific IgE antibodies and that the prevalence is seasonably variable. Our data indicate that persons recently stung without significant reaction contribute to this group but that only a small portion of this group is at risk for a systemic reaction with a future sting.

  15. Rapid desensitization induces internalization of antigen-specific IgE on mouse mast cells.

    Science.gov (United States)

    Oka, Tatsuya; Rios, Eon J; Tsai, Mindy; Kalesnikoff, Janet; Galli, Stephen J

    2013-10-01

    Rapid desensitization transiently prevents severe allergic reactions, allowing administration of life-saving therapies in previously sensitized patients. However, the mechanisms underlying successful rapid desensitization are not fully understood. We sought to investigate whether the mast cell (MC) is an important target of rapid desensitization in mice sensitized to exhibit IgE-dependent passive systemic anaphylaxis in vivo and to investigate the antigen specificity and underlying mechanisms of rapid desensitization in our mouse model. C57BL/6 mice (in vivo) or primary isolated C57BL/6 mouse peritoneal mast cells (PMCs; in vitro) were passively sensitized with antigen-specific anti-2,4-dinitrophenyl IgE, anti-ovalbumin IgE, or both. MCs were exposed over a short period of time to increasing amounts of antigen (2,4-dinitrophenyl-human serum albumin or ovalbumin) in the presence of extracellular calcium in vitro or by means of intravenous administration to sensitized mice in vivo before challenging the mice with or exposing the PMCs to optimal amounts of specific or irrelevant antigen. Rapidly exposing mice or PMCs to progressively increasing amounts of specific antigen inhibited the development of antigen-induced hypothermia in sensitized mice in vivo and inhibited antigen-induced PMC degranulation and prostaglandin D2 synthesis in vitro. Such MC hyporesponsiveness was induced antigen-specifically and was associated with a significant reduction in antigen-specific IgE levels on MC surfaces. Rapidly exposing MCs to progressively increasing amounts of antigen can both enhance the internalization of antigen-specific IgE on the MC surface and also desensitize these cells in an antigen-specific manner in vivo and in vitro. Copyright © 2013 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

  16. Influence of protein expression system on elicitation of IgE antibody responses: experience with lactoferrin.

    Science.gov (United States)

    Almond, Rachael J; Flanagan, Brian F; Kimber, Ian; Dearman, Rebecca J

    2012-11-15

    With increased interest in genetically modified (GM) crop plants there is an important need to understand the properties that contribute to the ability of such novel proteins to provoke immune and/or allergic responses. One characteristic that may be relevant is glycosylation, particularly as novel expression systems (e.g. bacterial to plant) will impact on the protein glycoprofile. The allergenicity (IgE inducing) and immunogenicity (IgG inducing) properties of wild type native human lactoferrin (NLF) from human milk (hm) and neutrophil granules (n) and a recombinant molecule produced in rice (RLF) have been assessed. These forms of lactoferrin have identical amino acid sequences, but different glycosylation patterns: hmNLF and nNLF have complex glycoprofiles including Lewis (Le)(x) structures, with particularly high levels of Le(x) expressed by nNLF, whereas RLF is simpler and rich in mannose residues. Antibody responses induced in BALB/c strain mice by intraperitoneal exposure to the different forms of lactoferrin were characterised. Immunisation with both forms of NLF stimulated substantial IgG and IgE antibody responses. In contrast, the recombinant molecule was considerably less immunogenic and failed to stimulate detectable IgE, irrespective of endotoxin and iron content. The glycans did not contribute to epitope formation, with equivalent IgE and IgG binding recorded for high titre anti-NLF antisera regardless of whether the immunising NLF or the recombinant molecule were used substrates in the analyses. These data demonstrate that differential glycosylation profiles can have a profound impact on protein allergenicity and immunogenicity, with mannose and Le(x) exhibiting opposing effects. These results have clear relevance for characterising the allergenic hazards of novel proteins in GM crops. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  17. Layered Electrical Product Application Protocol (AP). Draft: Initial Graphics Exchange Specification (IGES)

    Energy Technology Data Exchange (ETDEWEB)

    1994-09-01

    An application protocol is an information systems engineering view of a specific product. The view represents an agreement on the generic activities needed to design and fabricate the product, the agreement on the information needed to support those activities, and the specific constructs of a product data standard for use in transfering some or all of the information required. This applications protocol describes the data for electrical and electronic products in terms of a product description standard called the Initial Graphics Exchange Specification (IGES). More specifically, the Layered Electrical Product IGES Application Protocol (AP) specifies the mechanisms for defining and exchanging computer-models and their associated data for those products which have been designed in two dimensional geometry so as to be produced as a series of layers in IGES format. The AP defines the appropriateness of the data items for describing the geometry of the various parts of a product (shape and location), the connectivity, and the processing and material characteristics. Excluded is the behavioral requirements which the product was intended to satisfy, except as those requirements have been recorded as design rules or product testing requirements.

  18. Atopic dermatitis: immune deviation, barrier dysfunction, IgE autoreactivity and new therapies

    Directory of Open Access Journals (Sweden)

    Masutaka Furue

    2017-07-01

    Full Text Available Atopic dermatitis (AD is a chronic or chronically relapsing, eczematous, severely pruritic skin disorder mostly associated with IgE elevation and skin barrier dysfunction due to decreased filaggrin expression. The lesional skin of AD exhibits Th2- and Th22-deviated immune reactions that are progressive during disease chronicity. Th2 and Th22 cytokines further deteriorate the skin barrier by inhibiting filaggrin expression. Some IgEs are reactive to self-antigens. The IgE autoreactivity may precipitate the chronicity of AD. Upon activation of the ORAI1 calcium channel, atopic epidermis releases large amounts of thymic stromal lymphopoietin (TSLP, which initiates the Th2 and Th22 immune response. Th2-derived interleukin-31 and TSLP induce an itch sensation. Taken together, TSLP/Th2/Th22 pathway is a promising target for developing new therapeutics for AD. Enhancing filaggrin expression using ligands for the aryl hydrocarbon receptor may also be an adjunctive measure to restore the disrupted barrier function specifically for AD.

  19. Brain Abscess and Keratoacanthoma Suggestive of Hyper IgE Syndrome

    Directory of Open Access Journals (Sweden)

    Soheyla Alyasin

    2015-01-01

    Full Text Available Hyper immunoglobulin-E (IgE syndrome is an autosomal immune deficiency disease. It is characterized by an increase in IgE and eosinophil count with both T-cell and B-cell malfunction. Here, we report an 8-year-old boy whose disease started with an unusual skin manifestation. When 6 months old he developed generalized red, nontender nodules and pathologic report of the skin lesion was unremarkable (inflammatory. Then he developed a painless, cold abscess. At the age of 4 years, he developed a seronegative polyarticular arthritis. Another skin biopsy was taken which was in favor of Keratoacanthoma. Laboratory workup for immune deficiency showed high eosinophil count and high level of immunoglobulin-E, due to some diagnostic criteria (NIH sores: 41 in 9-year-olds, he was suggestive of hyper IgE syndrome. At the age of 8, the patient developed an abscess in the left inguinal region. While in hospital, the patient developed generalized tonic colonic convulsion and fever. Brain computed tomography scan revealed an abscess in the right frontal lobe. Subsequently magnetic resonance imaging (MRI of the brain indicated expansion of the existing abscess to contralateral frontal lobe (left side. After evacuating the abscesses and administrating intravenous antibiotic, the patient’s condition improved dramatically and fever stopped.

  20. Serum IgE Antibodies against Hazelnut in Hazelnut Processing Workers

    Directory of Open Access Journals (Sweden)

    Ege Gulec Balbay

    2012-01-01

    Full Text Available Aim. Previous studies have shown a higher sensitization rate to hazelnut in processing workers but no relation was found between the respiratory symptoms in workplace and hazelnut sensitization. Material and Method. To evaluate the association between the hazelnut sensitization and workplace-related respiratory complaints, hazelnut processing workers had undergone a questionnaire included work-related respiratory symptoms, smoking history, pulmonary function testing, and measurement of serum IgE antibodies against hazelnut. Results. This study consisted of 88 hazelnut processing workers (79 females and 9 males, aged 14–59 years (Mean ± SD: years. The mean working duration was months (min: 1–max: 180. Specific IgE against hazelnut allergens was positive in 14 of cases (17.1%. There was no significant difference between the cases with and without specific IgE against hazelnut allergens regarding respiratory symptoms, history of allergy, smoking status and spirometric values. Conclusion. 17.1% of the hazelnut processing workers were seropositive against hazelnut. Being sensitized to hazelnut was not found to be associated with work-related respiratory symptoms in this study. Further studies are needed in hazelnut workers respiratory health to search topics other than asthma.

  1. IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease

    Directory of Open Access Journals (Sweden)

    Peng-Xiang Zhao

    2018-01-01

    Full Text Available AIM: To reveal the cytokines involved in idiopathic orbital inflammatory disease (IOID and the relationship between Th17 cells, IgE and IOID pathogenesis. METHODS: Whole blood samples were processed immediately after collection and serological IgG4, IgG, and IgE antibodies were tested using ELISA. IOID and orbital cavernous hemangioma (CH tissue samples underwent Bio-Plex multiplex cytokine detection. Hematoxylin-Eosin (HE staining of all paraffin samples suggested the histological features of IOIDs, and expressions of IgG4 and IL-17A in affected tissues were detected by immunohistochemistry. RESULTS: Among 40 IOID plasma samples, 52.5% (21/40 were positive for IgG4 and 25% (10/40 were positive for IgE. Overlapped IgG4 or IgE positive samples accounted for 22.5% (9/40. Therefore, IOID samples were separated into three groups. The IgE+/IgG4+ group had a relevantly lower level of pro-inflammatory cytokine expression. IL-4 (Th2 cell related, IL-10 and TGF-β1 (Treg cell immunity related were elevated in all three groups. Some of the Th17 cell related cytokines (i.e. IL-17A/F, IL-25, IL-23, and IL-33 displayed higher expression levels in the IgE-/IgG4- group compared to the other two groups. CONCLUSION: We discovered an IgG4-IgE co-positive group as well as Th17 cell immune involvement in IgG4-IgE co-negative subgtroup in IOID for the first time. The pathogenesis of IOID could differ from different subgroups according to the IgG4 and IgE detection. Therefore, we recommend that, Treatment stratagy should be made according to the clinical assessment of IgG4-IgE and Th17 profile detection.

  2. Comparison of serum concentrations of environmental allergen-specific IgE in atopic and healthy (nonatopic) horses.

    Science.gov (United States)

    Wilkołek, P; Sitkowski, W; Szczepanik, M; Adamek, Ł; Pluta, M; Taszkun, I; Gołyński, M; Malinowska, A

    2017-12-01

    Allergic responses in humans, horses and other species are mediated by immunoglobulin E (IgE) antibodies. Serum testing to detect allergen-specific IgE antibodies has been developed for dogs, cats and horses; this allows for the identification of allergens and determination of appropriate allergen- specific immunotherapies. This study compared serum allergen-specific IgE concentrations in atopic and healthy horses. The study was performed on Malopolski breed atopic (n=21) and nonatopic (n=21) clinically healthy horses. Allergen-specific IgE serum concentrations were measured in summer seasons of 2008-2015 using a monoclonal anti-IgE antibody. A Northern and Central European allergen panel containing mite, insect, mould and plant pollen allergens, including 15 tests of individual allergens and 5 tests of allergen mixtures was used. The mean allergen-specific IgE concentrations in the atopic and normal horse populations were compared. Among the atopic horses, the strongest positive reactions occurred against the storage mites Tyrophagus putrescentiae and the domestic mite Dermatophagoides farinae. The atopic horses also demonstrated high IgE concentrations against insects, particularly Tabanus sp., the plant pollens colza, cultivated rye and the mould pollen mixture Aspergillus/Penicillium. No horses in the atopic group were IgE-negative. Among all mite, insect, mould and some plant allergen groups the differences in mean specific IgE concentrations between allergic and healthy horses were significant. The mean IgE concentrations for most allergen groups were significantly higher in the atopic horses than in the healthy animals. However, a high incidence of positive reactions was observed in both healthy and allergic horses. Our results showed a high frequency of polysensitization in atopic horses. Copyright© by the Polish Academy of Sciences.

  3. Measurement of anti-Ascaris IgE antibody levels in tropical allergic patients, using modified ELISA.

    Science.gov (United States)

    Lynch, N R; Pérez, M; López, R I; Turner, K J

    1987-01-01

    The two most common situations in which the determination of serum immunoglobulin E (IgE) levels is of interest are allergic disease and helminthic infection. This is of particular importance in the tropical environment, as helminthiasis possibly influences the expression of allergic reactivity. Because of the low absolute serum levels of IgE, solid-phase radioimmunoassay (RIA) is conventionally used for its measurement. The radioactive and toxic volatile reagents required restricted application of such assays in the tropical situation. We evaluated a nitrocellulose-based, avidin biotin-amplified enzyme-linked immunosorbent assay (ELISA) for IgE, in which monoclonal anti-IgE antibodies were employed. Excellent correlations were obtained between ELISA and RIA for both total and allergen-specific IgE measurement. The ELISA was then applied to determine the levels of anti-Ascaris antibodies in selected allergic patients, in whom no cutaneous immediate hypersensitivity reactions were demonstrated against common environmental allergens such as house dust, but who had positive skin reactions to Ascaris extract. When compared with non-allergic subjects who had equivalent cutaneous reactivity, no significant differences were found in total IgE levels, house-dust specific IgE levels or non-reaginic anti-Ascaris antibody levels. However, higher levels of IgE antibody against the parasite were detected in the allergic subjects. This observation raises the question of the possible role of Ascaris infection in the stimulation of allergic reactions in such patients. We describe an immunoenzymatic assay for total and specific IgE antibody that is better adapted to the tropical situation than the commonly used radioimmunoassays.(ABSTRACT TRUNCATED AT 250 WORDS)

  4. Chinese herbal extracts of Rubia cordifolia and Dianthus superbus suppress IgE production and prevent peanut-induced anaphylaxis

    Directory of Open Access Journals (Sweden)

    Yang Nan

    2011-09-01

    Full Text Available Abstract Background Peanut allergy is characterized by increased levels of peanut-specific IgE in the serum of most patients. Thus, the most logical therapy would be to inhibit the IgE production by committed B-cells. This study aims to investigate the unreported anti-IgE effects of Chinese herbal extracts of Rubia cordifolia (Qiancao and Dianthus superbus (Qumai. Methods Seventy herbal extracts were tested for their ability to reduce IgE secretion by a human B-cell line. Those with the lowest inhibitory concentration 50 (IC50 values were tested in a mouse model of peanut-anaphylaxis. Anaphylactic scores, body temperature, plasma histamine and peanut-specific-immunoglobulins were determined. Results Rubia cordifolia and Dianthus superbus inhibited the in vitro IgE production by a human B-cell line in a dose-dependent manner and the in vivo IgE production in a murine model of peanut allergy without affecting peanut-specific-IgG1 levels. After challenge, all mice in the sham groups developed anaphylactic reactions and increased plasma histamine levels. The extract-treated mice demonstrated significantly reduced peanut-triggered anaphylactic reactions and plasma histamine levels. Conclusion The extracts of Rubia cordifolia and Dianthus superbus inhibited the IgE production in vivo and in vitro as well as reduced anaphylactic reactions in peanut-allergic mice, suggesting potentials for allergy treatments.

  5. Chinese herbal extracts of Rubia cordifolia and Dianthus superbus suppress IgE production and prevent peanut-induced anaphylaxis

    Science.gov (United States)

    2011-01-01

    Background Peanut allergy is characterized by increased levels of peanut-specific IgE in the serum of most patients. Thus, the most logical therapy would be to inhibit the IgE production by committed B-cells. This study aims to investigate the unreported anti-IgE effects of Chinese herbal extracts of Rubia cordifolia (Qiancao) and Dianthus superbus (Qumai). Methods Seventy herbal extracts were tested for their ability to reduce IgE secretion by a human B-cell line. Those with the lowest inhibitory concentration 50 (IC50) values were tested in a mouse model of peanut-anaphylaxis. Anaphylactic scores, body temperature, plasma histamine and peanut-specific-immunoglobulins were determined. Results Rubia cordifolia and Dianthus superbus inhibited the in vitro IgE production by a human B-cell line in a dose-dependent manner and the in vivo IgE production in a murine model of peanut allergy without affecting peanut-specific-IgG1 levels. After challenge, all mice in the sham groups developed anaphylactic reactions and increased plasma histamine levels. The extract-treated mice demonstrated significantly reduced peanut-triggered anaphylactic reactions and plasma histamine levels. Conclusion The extracts of Rubia cordifolia and Dianthus superbus inhibited the IgE production in vivo and in vitro as well as reduced anaphylactic reactions in peanut-allergic mice, suggesting potentials for allergy treatments. PMID:21961957

  6. Total serum IgE level influences oral food challenge tests for IgE-mediated food allergies.

    Science.gov (United States)

    Horimukai, K; Hayashi, K; Tsumura, Y; Nomura, I; Narita, M; Ohya, Y; Saito, H; Matsumoto, K

    2015-03-01

    Probability curves predicting oral food challenge test (OFC) results based on specific IgE levels are widely used to prevent serious allergic reactions. Although several confounding factors are known to affect probability curves, the main factors that affect OFC outcomes are currently unclear. We hypothesized that an increased total IgE level would reduce allergic reactivity. Medical records of 337 and 266 patients who underwent OFCs for 3.5 g boiled hen's egg white and 3.1 ml raw cow's milk, respectively, were examined retrospectively. We subdivided the patients into three groups based on total IgE levels and age by percentile (75th percentiles), and logistic regression analyses were performed on each group. Patients with higher total IgE levels were significantly less responsive. In addition, age did not significantly affect the OFC results. Therefore, total IgE levels should be taken into account when predicting OFC results based on food-specific IgE levels. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  7. 98 Specific IGE and IGG Binding to Allergoids of Phleum pratense

    Science.gov (United States)

    Cases, Barbara; Fernandez-Caldas, Enrique; Tudela, Jose Ignacio; Fernandez, Eva Abel; Sanchez-Garcia, Silvia; Ibañez, M. Dolores; Escudero, Carmelo; Casanovas, Miguel

    2012-01-01

    Background Allergoids were first used in the decades of the 60s and 70s of the last century as an effective treatment of allergic respiratory diseases. Allergoids can be modified with formaldehyde or glutaraldehyde. Modified allergens, or allergoids, decrease the risk of adverse reactions while administering higher allergen doses. The objective of this study was to analyse specific IgE and IgG binding to glutaraldehyde modified and non-modified allergen extracts of Phleum pratense. Methods The sera of 69 patients sensitized to P. pratense were tested. All these patients had signs and symptoms of rhinoconjunctivitis with, or without, asthma in May and June of 2011. All these patients had positive skin prick tests to a standardized extract of P. pratense, and other grass species. Most patients were also sensitized to olive pollen. Specific IgE and IgG binding were analysed by direct ELISA against P. pratense native (non-modified) and allergoid extracts. Relative potencies were evaluated through ELISA inhibition assays, and the protein composition of non-modified and allergoid samples was determined by Mass Spectrometry (MS/MS). Results Mean Specific IgE levels against the native extract was 16.68 ± 11.65 Units (U) and against the allergoid: 7.26 ± 8.24 U (P allergoid (P = 0.16; Mann-Whitney). Linear regression coefficients obtained between immunoglobulin reactivity against both extracts were: r2 = 0.51 for specific IgE and r2 = 0.83 for specific IgG. An important decrease in the allergenic activity, measured by inhibition ELISA, was clearly observed. The MS/MS assay revealed the presence of the mayor allergen, and some isoforms, in non-modified and allergoid extracts. Conclusions Results obtained demonstrate that the glutaraldehyde polymerization process induces an important decrease in specific IgE binding to allergoids of P. pratense while there are no significant differences in specific IgG binding. The allergenic composition of the P. pratense allergoid was

  8. Asma alérgica, niveles de IgE total y exposición a los acaros del polvo casero en el municipio de Santiago de Tolú, Colombia

    Directory of Open Access Journals (Sweden)

    Salomón Rodríguez Monterroza

    2013-10-01

    Full Text Available ResumenLa creciente prevalencia del asma a nivel mundial hace necesaria la investigación de factores que influyen sobre su etiologia y patogénesis. En Colombia la prevalencia de asma es tan alta como en los paises industrializados. Un estudio de historias dinicos en el municipio de Santiago de Tolú (Colombia, mostró un alto porcentaje de consulta por asma y rinitis alérgica en la población infantil. Con el objetivo de determinarla frecuencia de asma en la población general se realizó un estudio descriptivo de corte transversal, partiendo de la información recolectada con un cuestionario diseñado y probado. Se encuestaron 316 personas, con representatividad de grupos etéreos y estratos socioeconómicos. El diagnóstico fue confirmado mediante espirometria y medición de los niveles de ige total en sangre. Como resultado se encontró que 8,9% de la población mostró sintomas clinicos y espiromet ría positiva paro  asma con niveles de ige total > 100 Ul/ml, siendo los niños entre 5 y 15 años los más afectados (71.5%. La encuesta permitió evaluar la sensibilidad a factores ambientales, siendo el polvo casero y el humo del cigarrillo los más asociados a la enfermedad. La presencia de ácaros en el polvo acumulado en colchones be confirmada, siendo Dennatophagoides pteronyssinusy Momia tropicalis las especies más frecuen-tes. (Duazary 2006; 1: 10- 17 

  9. Clinical evaluation of total IgE in tears of patients with allergic conjunctivitis disease using a novel application of the immunochromatography method.

    Science.gov (United States)

    Inada, Noriko; Shoji, Jun; Kato, Hiroshi; Kiely, Surayah; Mulyanto; Sawa, Mitsuru

    2009-12-01

    The determination of total IgE in tears is useful as a diagnostic tool in allergic conjunctivitis disease (ACD). We evaluated the efficacy of this diagnostic tool for ACD, which is a clinically applicable novel immunochromagraphic method to determine total IgE in tears. The subjects comprised 4 groups: 15 patients with vernal keratoconjunctivitis (VKC group), 8 patients with atopic keratoconjunctivitis (AKC group), 18 patients with allergic conjunctivitis (AC group), and 7 normal healthy volunteers as a control (control group). Tears were sampled using filter paper, and the total IgE in tears was determined by immunochromatography assay. Semiquantitative determination was carried out by examining the intensity of the colored line using an immunochromatoreader (IgE index). The relationship between IgE indices in tears and total IgE levels in serum or between IgE indices and the clinical scores of ACD was examined. The positive ratio obtained by this novel application of the immunochromatography assay was 38 of the 41 in the patients with ACD and none in the 7 controls. IgE indices for the VKC group, AKC group and AC group were 27.5 +/- 15.6, 19.8 +/- 15.8, and 4.0 +/- 3.1 (mean +/- SD), respectively. IgE indices in tears showed significant correlation with both total IgE levels in serum (P tears is a useful clinical tool to investigate ACD.

  10. Regulation of IgE antibody production by serum molecules. I. Serum from complete Freund's adjuvant-immune donors suppresses irradiation-enhanced IgE production in low responder mouse strains

    International Nuclear Information System (INIS)

    Tung, A.S.; Chiorazzi, N.; Katz, D.H.

    1978-01-01

    Exposure of mice to low doses of x irradiation at or near the time of primary immunization with 2,4-dinitrophenyl (DNP)-Ascaris suum extract (ASC) results in substantial enhancement of IgE anti-DNP antibody responses; the IgG antibody responses of such mice do not increase after such manipulations. This selective enhancement of IgE antibody production occurs in mice of both high and low IgE responder phenotype, although the extent of enhancement compared to unmanipulated control animals is more striking in low IgE responder mice. The studies presented here demonstrate that the irradiation-enhanced IgE antibody responses of low responder SJL and C57BL/6 mice as well as of intermediate responder AKR mice can be effectively suppressed by passive transfer of CFA-immune serum obtained from isologous donor mice. Moreover, adoptive secondary IgE antibody responses in SJL recipients of primed syngeneic spleen cells can be totally abolished by passive transfer of CFA-immune serum or ascitic fluid from CFA-immune mice. The suppressive activity of CFA-immune serum can be diminished or eliminated by exposure of CFA-primed donor mice to low dose x irradiation at an appropriate point during the priming regimen, after a single inoculation of CFA, and before collection of serum. Low dose x irradiation was not effective in eliminating suppressive activity of CFA-induced ascites fluid obtained from donor mice inoculated repeatedly with CFA. In contrast to the capacity of CFA-immune serum from isologous donors to suppress irradiation-enhanced IgE responses of low responder mice, similar sera or ascites fluids were ineffective in suppressing irradiation-enhanced responses of high responder BALB/c or (SJL x BALB/c)F 1 hybrid mice

  11. Relaciones sociales en el empleo, en trabajadores con discapacidad

    Directory of Open Access Journals (Sweden)

    Pilar Ibáñez

    2005-01-01

    Full Text Available El propósito de este estudio es aproximarnos al conocimiento del perfil y las relaciones sociales de 252 personas con distintos tipos de discapacidad, en el contexto del emplea protegido, a través de un cuestionario de autovaloración. Los resultados obtenidos ponen de manifiesto que en el empleo protegido predominan los trabajadores -sin diferencias significativas respecto al género- con discapacidad psíquica y edad media de 31 años. Estas características no influyen significativamente en el gusto por su trabajo, su ambiente laboral o su capacidad para hacer amigos dentro del trabajo. Las personas con discapacidad psíquica tienen un número significativamente menor de amigos fuera del trabajo. Este último aspecto sí está significativamente relacionado con tener amigos dentro del contexto laboral. Por otra parte, encontramos elevadas expectativas de autoeficacia y predisposición al cambio ocupacional.

  12. Biofiltración del gas natural cubano en soporte orgánico con lodos activos

    OpenAIRE

    Díaz-Rodríguez, Yuletsis; Casellas Ortega, Raidel; Rodríguez Muñoz, Susana; Fernández Santana, Elina; Mustelier Pérez, Sergio Pascual

    2016-01-01

    El uso del gas natural cubano se afecta por su elevada concentración de H2S. De ahí, la búsqueda de nuevas opciones de tratamiento como los procesos biológicos y el uso de materias primas naturales, renovables y biodegradables para tales fines. Con el objetivo de evaluar la fibra de kenaf como material de empaque, en biofiltro escurrido con lodos activos, para la desulfuración del gas natural; se realizó la caracterización físico-química de la fibra así como pruebas hidrodinámicas. La efectiv...

  13. Farmacocinética y farmacodinamia de antimicrobianos: a propósito de pacientes con neutropenia y fiebre

    OpenAIRE

    Garzón, Javier R; Cuervo M, Sonia; Gómez R, Julio; Cortés, Jorge A

    2011-01-01

    La neutropenia febril es una complicación grave de la terapia antineoplásica que se presenta más frecuentemente en pacientes con neoplasias hematológicas, asociada a tasas elevadas de mortalidad. Uno de los factores descritos como causa de fracasos terapéuticos de la terapia antimicrobiana es la inadecuada concentración tisular de los antimicrobianos que a su vez se correlaciona con bajas concentraciones en el líquido intersticial en el caso de los fármacos hidrofílicos. En pacientes críticam...

  14. Mast cells and IgE in defense against venoms: Possible “good side” of allergy?

    Directory of Open Access Journals (Sweden)

    Stephen J. Galli

    2016-01-01

    Full Text Available Physicians think of mast cells and IgE primarily in the context of allergic disorders, including fatal anaphylaxis. This ‘bad side’ of mast cells and IgE is so well accepted that it can be difficult to think of them in other contexts, particularly those in which they may have beneficial functions. However, there is evidence that mast cells and IgE, as well as basophils (circulating granulocytes whose functions partially overlap with those of mast cells, can contribute to host defense as components of adaptive type 2 immune responses to helminths, ticks and certain other parasites. Accordingly, allergies often are conceptualized as “misdirected” type 2 immune responses, in which IgE antibodies are produced against any of a diverse group of apparently harmless antigens, as well as against components of animal venoms. Indeed, certain unfortunate patients who have become sensitized to venoms develop severe IgE-associated allergic reactions, including fatal anaphylaxis, upon subsequent venom exposure. In this review, we will describe evidence that mast cells can enhance innate resistance to reptile or arthropod venoms during a first exposure to such venoms. We also will discuss findings indicating that, in mice which survive an initial encounter with venom, acquired type 2 immune responses, IgE antibodies, the high affinity IgE receptor (FcɛRI, and mast cells can contribute to acquired resistance to the lethal effects of both honeybee venom and Russell's viper venom. These findings support the hypothesis that mast cells and IgE can help protect the host against venoms and perhaps other noxious substances.

  15. Effects of nasal corticosteroids on boosts of systemic allergen-specific IgE production induced by nasal allergen exposure.

    Directory of Open Access Journals (Sweden)

    Cornelia Egger

    Full Text Available Allergen exposure via the respiratory tract and in particular via the nasal mucosa boosts systemic allergen-specific IgE production. Intranasal corticosteroids (INCS represent a first line treatment of allergic rhinitis but their effects on this boost of allergen-specific IgE production are unclear.Here we aimed to determine in a double-blind, placebo-controlled study whether therapeutic doses of an INCS preparation, i.e., nasal fluticasone propionate, have effects on boosts of allergen-specific IgE following nasal allergen exposure.Subjects (n = 48 suffering from grass and birch pollen allergy were treated with daily fluticasone propionate or placebo nasal spray for four weeks. After two weeks of treatment, subjects underwent nasal provocation with either birch pollen allergen Bet v 1 or grass pollen allergen Phl p 5. Bet v 1 and Phl p 5-specific IgE, IgG1-4, IgM and IgA levels were measured in serum samples obtained at the time of provocation and one, two, four, six and eight weeks thereafter.Nasal allergen provocation induced a median increase to 141.1% of serum IgE levels to allergens used for provocation but not to control allergens 4 weeks after provocation. There were no significant differences regarding the boosts of allergen-specific IgE between INCS- and placebo-treated subjects.In conclusion, the application of fluticasone propionate had no significant effects on the boosts of systemic allergen-specific IgE production following nasal allergen exposure.http://clinicaltrials.gov/NCT00755066.

  16. Macrophage triggering by aggregated immunoglobulins. II. Comparison of IgE and IgG aggregates or immune complexes.

    Science.gov (United States)

    Pestel, J; Dessaint, J P; Joseph, M; Bazin, H; Capron, A

    1984-01-01

    Macrophages incubated with complexed or aggregated IgE released beta-glucuronidase (beta-G) within 30 min. In contrast in the presence of aggregated or complexed IgG, macrophages liberated equivalent amount of beta-G only after 6 h incubation. In addition the rapid macrophage stimulation induced by aggregated IgE was also followed by a faster 3H-glucosamine incorporation when compared to the delayed activation caused by aggregated IgG. However, macrophages stimulated either by IgG or by IgE oligomers produced the same percentage of plasminogen activator at 24 h. In contrast, while the interaction between macrophages and aggregated IgE was only followed by a peak of cyclic GMP and a beta-G release during the first 30 min of incubation, the interaction between macrophages and IgG oligomers was accompanied by a simultaneous increase of cyclic GMP and AMP nucleotides and by an absence of beta-G exocytosis. Moreover, the beta-G release induced by aggregated IgE was increased when macrophages were preincubated with aggregated IgG. This additive effect was not observed in the reverse situation. Finally macrophages activated by IgG oligomers were demonstrated to exert a cytotoxic effect on tumour cells and to kill schistosomula in the presence of a low level of complement. Taken together these results underline the peculiar ability of aggregated or complexed IgE to trigger rapidly the macrophage activation compared to aggregated IgG and can explain the important role of complexed IgE in some macrophage dependent cytotoxicity mechanisms (i.e. in parasitic diseases). PMID:6088135

  17. Isoforms of the major peanut allergen Ara h 2: IgE binding in children with peanut allergy.

    Science.gov (United States)

    Hales, Belinda J; Bosco, Anthony; Mills, Kristina L; Hazell, Lee A; Loh, Richard; Holt, Patrick G; Thomas, Wayne R

    2004-10-01

    The major peanut allergen Ara h 2 consists of two isoforms, namely Ara h 2.0101 and Ara h 2.0201. The recently identified Ara h 2.0201 isoform contains an extra 12 amino acids including an extra copy of the reported immunodominant epitope DPYSPS. This study aimed to evaluate the IgE binding of the two Ara h 2 isoforms. Ten clones of Ara h 2 were sequenced to assess the relative frequency of the Ara h 2 isoforms and to identify whether there was further variation in the Ara h 2 sequence. IgE binding to Ara h 2.0101 and Ara h 2.0201 was measured for 70 peanut-allergic children using an IgE DELFIA assay to quantitate specific IgE binding. A competition assay was used to measure whether Ara h 2.0201 contained IgE epitopes other than those found for Ara h 2.0101. The original Ara h 2.0101 sequence was found for 6/10 clones and Ara h 2.0201 was found for 2/10 clones. Ara h 2.0201 had the expected insertion of 12 amino acids as well as substitutions at positions 40 (40G) and 142 (142E). Two new isoforms were identified as different polymorphisms of position 142. One Ara h 2.01 clone (Ara h 2.0102) contained 142E and one Ara h 2.02 clone (Ara h 2.0202) contained 142D. A polymorphism that was previously identified by other investigators at position 77 (77Q or 77R) was not found for any of the 10 sequences. Although the level of IgE binding to Ara h 2.0201 of individual patients was frequently higher than the binding to Ara h 2.0101 (p originally sequenced Ara h 2.0101 isoform and contains other IgE specificities.

  18. Estabilización de los indicadores hematológicos en paciente con policitemia absoluta secundaria tratado con Vimang

    Directory of Open Access Journals (Sweden)

    Gregorio Martínez-Sánchez

    2009-04-01

    Full Text Available Se reporta un caso del sexo masculino, de 72 años de edad con antecedentes de hipertensión arterial (con un período de evolución de 5 años que fue remitido en el 2001 a la Consulta de Hematología por presentar cifras elevadas de hemoglobina (Hb y hematócrito (Hto. Las cifras elevadas de Hb y Hto se detectaron durante un chequeo médico como resultado de que el paciente presentara pérdida de conocimiento y fuera hospitalizado. El paciente se remitió al Instituto de Hematología e Inmunología (Ciudad de La Habana para realizarle un estudio de volemia y ferrocinética, el cual mostró que presentaba policitemia absoluta moderada. Se descartó policitemia vera y se buscaron causas de policitemia absoluta secundaria sin que hayan encontrado ninguna hasta el presente. Desde este momento el paciente se ha seguido en la Consulta de Hematología del Hospital General Docente Ernesto Che Guevara (Las Tunas y cada 6 meses es chequeado en el Instituto de Hematología e Inmunología, Ciudad de La Habana. A partir del mes de abril de 2004 el paciente recibió 600 mg de Vimang® (extracto de Mangifera indica L. y se incrementó 2 meses después a 900 mg en 3 dosis separadas de 300 mg (tabletas diariamente. El presente estudio analizó la historia clínica del paciente y los resultados de todos los estudios que se le practicaron en los años precedentes al tratamiento con Vimang® y los observados durante el año que estaba bajo tratamiento. Como resultado se observó una reducción en un 50 % del número de flebotomías necesarias para mantener el paciente bajo control con respecto a los valores de Hto y Hb. Se encontraron además diferencias significativas (p < 0,05 con respecto a las desviaciones estándar de los indicadores Hto y Hb, que fueron menores en el periodo en que el paciente fue tratado con el extracto natural.

  19. Comparison between sensitivity of autologous skin serum test and autologous plasma skin test in patients with Chronic Idiopathic Urticaria for detection of antibody against IgE or IgE receptor (FcεRIα).

    Science.gov (United States)

    Sajedi, Vahid; Movahedi, Masoud; Aghamohammadi, Asghar; Aghamohamadi, Asghar; Gharagozlou, Mohammad; Ghareguzlou, Mohammad; Shafiei, Alireza; Soheili, Habib; Sanajian, Nahal

    2011-06-01

    Intradermal injection of autologous serum and plasma elicit a cutaneous reactivity in almost 45-60% of patients with Chronic Idiopathic Urticaria (CIU). This reactivity is associated with the presence of auto antibodies against IgE or IgE receptors. This study was carried out to compare the cutaneous reactivity of autologous serum and plasma skin tests in a series of patients with CIU for diagnosis of auto antibodies against IgE or IgE receptor. Fifty eight patients with CIU were injected intradermally with autologous serum and plasma (anticoagulated by citrate). Histamine was used as positive control and normal saline as negative control. The study group was checked by routine laboratory tests (CBC, U/A etc), allergens with skin prick tests, and serum IgE level, and auto antibodies against thyroid as well. Duration of urticaria was another factor which was assessed.There was no significant difference between positive ASST and positive APST patients for the above mentioned tests. 77.6% of the patients were Positive for APST and 65.5% were ASST positive. Duration of urticaria was longer in patients with positive ASST and APST than ASST and APST negative patients, although the difference was not statistically significant.Autologus serum skin test (ASST) and autologous plasma skin test (APST) could be used for estimation of duration and severity of urticaria and planning for the treatment.

  20. Human tonsillar IgE biosynthesis in vitro. I. Enhancement of IgE and IgG synthesis in the presence of pokeweed mitogen by T-cell irradiation

    International Nuclear Information System (INIS)

    Ohta, K.; Manzara, T.; Harbeck, R.J.; Kirkpatrick, C.H.

    1982-01-01

    A study of the events regulating human IgE biosynthesis in vitro was undertaken with tonsillar lymphocytes. IgG synthesis was also studied to evaluate the specificity of our observations. T-cell irradiation significantly enhanced synthesis of IgE by pokeweed mitogen (PWM)-stimulated B cells from 12 of 18 donors and IgG in all 18 donors. This enhancement was the result of de novo immunoglobulin synthesis, since the amount of IgE and IgG spontaneously released from lysed and lysed-and-cultured mononuclear cells was significantly less than that detected in the cell cultures, and the augmentation was completely ablated by the treatment of the cells with cycloheximide or mitomycin C. Enhancement was also dependent on the presence of PWM; T-cell irradiation did not enhance IgE synthesis in unstimulated cultures. Moreover, this enhancement was also observed in the co-cultures of B cells and allogeneic irradiated T cells. These observations suggest that radiosensitive T cells exert a suppressive activity that contributes to regulation of human IgE and IgG synthesis and that the suppressor function as well as the helper function can overcome allogeneic disparities

  1. Allergy to fish collagen: Thermostability of collagen and IgE reactivity of patients' sera with extracts of 11 species of bony and cartilaginous fish

    Directory of Open Access Journals (Sweden)

    Yukihiro Kobayashi

    2016-10-01

    Conclusions: The IgE reactivity of patients' sera to fish collagen in extracts was retained even when fish meat was treated by a high heating load. As for the fish collagen, the IgE reactivities to cartilaginous fish were lower than that to bony fish. Reducing IgE reactivity to fish meat using heat is difficult, and other modalities will be required to produce hypoallergenic fish meat.

  2. Differential IgE binding to isoallergens from Asian seabass (Lates calcarifer) in children and adults.

    Science.gov (United States)

    Sharp, Michael F; Kamath, Sandip D; Koeberl, Martina; Jerry, Dean R; O'Hehir, Robyn E; Campbell, Dianne E; Lopata, Andreas L

    2014-11-01

    Fish allergy is a common food allergy, with prevalence rates in the general population ranging between 0.2% and 2.3%. In both adults and children fish ranks in the top eight foods known to cause IgE mediated food allergy. Fish allergy is rarely outgrown and individuals with fish allergy may be allergic to some but not all species of fish. Whilst fish allergy occurs around the world, the characterization of allergenic components of individual species of fish has been largely confined to Northern hemisphere and European fish species. To date allergy to commonly consumed fish in the Asian-Pacific region including barramundi (Asian seabass; Lates calcarifer) have been less well investigated. The aim of this study was to identify and characterize allergenic proteins from barramundi in both fish allergic adult and pediatric patients. Serum from 17 fish allergic adults and children from Australia were characterized by immunoblotting and enzyme linked immunosorbent assays (ELISA) against raw and heated barramundi. Molecular analysis of identified allergens included genetic sequencing and generation of recombinant isoallergens. Two novel parvalbumin isoforms of the β-type were identified as the only allergens in barramundi and subsequently designated as Lat c 1.0101 and Lat c 1.0201 by the International Union of Immunological Societies. These two isoallergens do not differ in their ability to bind IgE antibodies, but are differentially expressed in barramundi tissue. This study characterized two novel heat stable parvalbumin allergens from barramundi, with differential IgE binding capacity between adults and pediatric patients. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. Prevalence of IgE against neuromuscular blocking agents in hairdressers and bakers.

    Science.gov (United States)

    Dong, S; Acouetey, D S; Guéant-Rodriguez, R-M; Zmirou-Navier, D; Rémen, T; Blanca, M; Mertes, P M; Guéant, J-L

    2013-11-01

    Allergic IgE-mediated reactions to neuromuscular blocking agents (NMBAs) are the main cause of immediate hypersensitivity reactions in anaesthesia; their predominant occurrence in the absence of previous exposure to NMBAs suggests a risk related to environmental exposure. To investigate the prevalence of specific IgE to quaternary ammonium ions in two populations professionally exposed to quaternary ammonium compounds, in the north-eastern France. The study had a retrospective follow-up design whereby apprentices were assessed after their 2-year training period as apprentices. The professionally exposed hairdresser populations (n = 128) were compared with baker/pastry makers (n = 108) and 'non-exposed' matched control subjects (n = 379). We observed a 4.6-fold higher frequency of positive IgE against quaternary ammonium ions in hairdressers (HD), compared with baker/pastry makers (BP) and control (C) groups. The competitive inhibition of quaternary ammonium Sepharose radioimmunoassay (QAS-IgE RIA) with succinylcholine was significantly higher in HD, compared with BP and C groups, with inhibition percentage of 66.2 ± 7.4, 39.7 ± 6.0 and 43.8 ± 9.9, respectively (P  100 kU/L were the two significant predictors of IgE-sensitization against quaternary ammonium ions in the multivariate analysis of a model that included age, sex, professional exposure, increased concentration of total IgE (IgE > 100 kU/L) and positive IgE against prevalent allergens (Phadiatop(®) ; P = 0.019 and P = 0.001, respectively). The exposure to hairdressing professional occupational factors increases IgE-sensitization to NMBAs and quaternary ammonium ion compounds used in hairdressing. Besides the pholcodine hypothesis, our study suggests that repetitive exposure to quaternary ammonium compounds used in hairdressing is a risk factor for NMBAs sensitization. © 2013 John Wiley & Sons Ltd.

  4. Transmembrane adaptor proteins in the high-affinity IgE receptor signaling

    Czech Academy of Sciences Publication Activity Database

    Dráber, Petr; Hálová, Ivana; Levi-Schaffer, F.; Dráberová, Lubica

    2012-01-01

    Roč. 2, 11.1. (2012), s. 95 ISSN 1664-3224 R&D Projects: GA MŠk 1M0506; GA ČR GA301/09/1826; GA ČR GAP302/10/1759; GA AV ČR KAN200520701 Grant - others:AV ČR(CZ) M200520901 Institutional research plan: CEZ:AV0Z50520514 Institutional support: RVO:68378050 Keywords : IgE receptor * LAT/LAT1 * LAX * NTAL/Lab/LAT2 * PAG/Cbp * mast cells * plasma membrane * transmembrane adaptor proteins Subject RIV: EB - Genetics ; Molecular Biology

  5. Rapid polyclonal desensitization with antibodies to IgE and FcεRIα.

    Science.gov (United States)

    Khodoun, Marat V; Kucuk, Zeynep Yesim; Strait, Richard T; Krishnamurthy, Durga; Janek, Kevin; Lewkowich, Ian; Morris, Suzanne C; Finkelman, Fred D

    2013-06-01

    Rapid desensitization, a procedure in which persons allergic to an antigen are treated at short intervals with increasing doses of that antigen until they tolerate a large dose, is an effective, but risky, way to induce temporary tolerance. We wanted to determine whether this approach can be adapted to suppress all IgE-mediated allergies in mice by injecting serially increasing doses of monoclonal antibodies (mAbs) to IgE or FcεRIα. Active and passive models of antigen- and anti-IgE mAb-induced IgE-mediated anaphylaxis were used. Mice were desensitized with serially increasing doses of anti-IgE mAb, anti-FcεRIα mAb, or antigen. Development of shock (hypothermia), histamine and mast cell protease release, cytokine secretion, calcium flux, and changes in cell number and FcεRI and IgE expression were evaluated. Rapid desensitization with anti-IgE mAb suppressed IgE-mediated immediate hypersensitivity; however, some mice developed mild anaphylaxis during desensitization. Rapid desensitization with anti-FcεRIα mAb that only binds FcεRI that is not occupied by IgE suppressed both active and passive IgE-mediated anaphylaxis without inducing disease. It quickly, but temporarily, suppressed IgE-mediated anaphylaxis by decreasing mast cell signaling through FcεRI, then slowly induced longer lasting mast cell unresponsiveness by removing membrane FcεRI. Rapid desensitization with anti-FcεRIα mAb was safer and longer lasting than rapid desensitization with antigen. A rapid desensitization approach with anti-FcεRIα mAb safely desensitizes mice to IgE-mediated anaphylaxis by inducing mast cell anergy and later removing all mast cell IgE. Rapid desensitization with an anti-human FcεRIα mAb may be able to prevent human IgE-mediated anaphylaxis. Copyright © 2013 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

  6. Confirmation of a predicted lack of IgE binding to Cry3Bb1 from genetically modified (GM) crops.

    Science.gov (United States)

    Nakajima, Osamu; Koyano, Satoru; Akiyama, Hiroshi; Sawada, Jun-Ichi; Teshima, Reiko

    2010-04-01

    Some GM crops including MON863 corn and stack varieties contain Cry3Bb1 protein. Cry3Bb1 is very important from the standpoint of assessing the safety of GM crops. In this study Cry3Bb1 was assessed from the standpoint of possible binding to IgE from allergy patients. First, an ELISA that was improved in our laboratory was used to test serum samples from 13 corn allergy patients in the United States with recombinant Cry3Bb1 expressed in Escherichia coli, and serum samples from 55 patients in Japan with various food allergies were also assayed. Two samples from the Japanese allergy patients were suspected of being positive, but Western blotting analysis with purified Cry3Bb1 indicated that the binding between IgE and Cry3Bb1 was nonspecific. Ultimately, no specific binding between IgE and recombinant Cry3Bb1 was detected. Next, all proteins extracted from MON863 corn and non-GM corn were probed with IgE antibodies in serum samples from the corn allergy patients by Western blotting, but the staining patterns of MON863 and non-GM corn were similar, meaning that unintended allergic reactions to MON863 are unlikely to occur. Our study provides additional information that confirms the predicted lack of IgE binding to Cry3Bb1 in people with existing food allergies. Copyright 2009 Elsevier Inc. All rights reserved.

  7. Mechanism underlying the suppressor activity of retinoic acid on IL4-induced IgE synthesis and its physiological implication.

    Science.gov (United States)

    Seo, Goo-Young; Lee, Jeong-Min; Jang, Young-Saeng; Kang, Seung Goo; Yoon, Sung-Il; Ko, Hyun-Jeong; Lee, Geun-Shik; Park, Seok-Rae; Nagler, Cathryn R; Kim, Pyeung-Hyeun

    2017-12-01

    The present study extends an earlier report that retinoic acid (RA) down-regulates IgE Ab synthesis in vitro. Here, we show the suppressive activity of RA on IgE production in vivo and its underlying mechanisms. We found that RA down-regulated IgE class switching recombination (CSR) mainly through RA receptor α (RARα). Additionally, RA inhibited histone acetylation of germ-line ε (GL ε) promoter, leading to suppression of IgE CSR. Consistently, serum IgE levels were substantially elevated in vitamin A-deficient (VAD) mice and this was more dramatic in VAD-lecithin:retinol acyltransferase deficient (LRAT -/- ) mice. Further, serum mouse mast cell protease-1 (mMCP-1) level was elevated while frequency of intestinal regulatory T cells (Tregs) were diminished in VAD LRAT -/- mice, reflecting that deprivation of RA leads to allergic immune response. Taken together, our results reveal that RA has an IgE-repressive activity in vivo, which may ameliorate IgE-mediated allergic disease. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Analysis of IgE binding proteins of mesquite (Prosopis juliflora) pollen and cross-reactivity with predominant tree pollens.

    Science.gov (United States)

    Dhyani, Anamika; Arora, Naveen; Gaur, Shailendra N; Jain, Vikram K; Sridhara, Susheela; Singh, Bhanu P

    2006-01-01

    Pollen from the mesquite tree, Prosopis juliflora, is an important source of respiratory allergy in tropical countries. Our aim was to partially characterize the IgE binding proteins of P. juliflora pollen extract and study cross-reactivity with prevalent tree pollen allergens. Intradermal tests with P. juliflora and five other tree pollen extracts were performed on respiratory allergy patients from Bikaner (arid) and Delhi (semi arid). Prosopis extract elicited positive skin reactions in 71/220 of the patients. Sera were collected from 38 of these 71 patients and all demonstrated elevated specific IgE to P. juliflora. Immunoblotting with pooled patients' sera demonstrated 16 IgE binding components, with components of 24, 26, 29, 31, 35, 52, 58, 66 and 95 kDa recognized by more than 80% of individual patients' sera. P. juliflora extract is allergenically potent requiring 73 ng of self-protein for 50% inhibition of IgE binding in ELISA inhibition. Cross-inhibition assays showed close relationship among P. juliflora, Ailanthus excelsa, Cassia siamea and Salvadora persica. IgE binding components of 14, 41, 52 and 66 kDa were shared allergens whereas 26 and 29 kDa were specific to P. juliflora. The findings suggest that purification of cross-reactive allergens will be helpful for diagnosis and immunotherapy of tree pollen allergic patients.

  9. Caso Clínico: Plan de cuidados al paciente con hemorragia subaracnoidea

    OpenAIRE

    Sánchez Galarraga, Iciar

    2016-01-01

    Trabajo fin de grado en Enfermería Introducción: La hemorragia subaracnoidea es una de las enfermedades cerebrovasculares que a pesar de ser poco frecuente en España, tiene una mortalidad elevada y produce secuelas que conllevan un gran impacto en la vida de quienes la padecen. Objetivo: Realizar un plan de cuidados estandarizado para el paciente con hemorragia subaracnoidea compleja. Material y Método: Durante el primer día de ingreso, se realiza una entrevista a cerca del estado de...

  10. Efectos del entrenamiento interválico en personas con insuficiencia cardiaca

    OpenAIRE

    Rollán Díaz, Ibai

    2014-01-01

    El objetivo de esta revisión es conocer en profundidad la patología de la insuficiencia cardiaca (IC), así como el tratamiento de la misma a través del entrenamiento aeróbico interválico de alta intensidad (HIIT). La IC se define como el estado fisiopatológico y clínico en el cual el corazón es incapaz de aportar sangre de acuerdo a los requerimientos metabólicos periféricos o que lo hace con presiones de llenado elevadas (Jalil, 2012). Resulta de especial relevancia el tratamiento de dicha ...

  11. Paciente con esquizofrenia tratado con ziprasidona + clozapina

    Directory of Open Access Journals (Sweden)

    Pol Yanguas E.

    2013-05-01

    Full Text Available P es un paciente diagnosticado de esquizofrenia, sigue en un piso tutelado un programa de rehabilitación, está medicado con clozapina 500 mg/día y ziprasidona 280 mg/ día. Padece hipercolesterolemia, tabaquismo y sus hábitos alimenticios no son buenos. La medicación que utiliza desde 2007 hasta ahora se refleja en la tabla 1. El último tratamiento se le introdujo el 7 de agosto de 2012, habiendo presentado un electro cardiograma (ECG normal, pero con ligera taquicardia ventricular y prolactinemia de 44,8 ng/ml (valores normales: 2-18 ng/ml.

  12. The Enhanced Radiocaesium Levels of People in Northern Sweden; Charge Corporelle de Radiocesium Relativement Elevie chez les Habitants de la Suede Septentrionale; 041f 041e 0414 0 ; Cargas de Radiocesio Anormalmente Elevadas en los Habitantes del Norte de Suecia

    Energy Technology Data Exchange (ETDEWEB)

    Liden, K.; Naversten, Y. [Radiation Physics Department, University of Lund (Sweden)

    1964-11-15

    Lapons, on a releve des charges corporelles de cesium 137 allant de 15 a 690 nc. En avril 1962, on a fait des dosages sur un autre groupe de 157 personnes de la meme region. Les charges de cesium 137 allaient alors de 30 a 740 nc. En avril 1963, une expedition qui, en cinq semaines, s'est rendue dans sept centres de la Suede septentrionale a fait des dosages sur un total de 458 personnes au moyen du meme appareil monte sur un autobus. Cette fois-la, les charges de cesium 137 chez les Lapons de cette region allaient de 50 a 900 nc. La charge corporelle moyenne'de cesium 137 pour tous les eleveurs de rennes - soit un groupe de 78 personnes - mesuree en avril 1963 s'elevait a 461 nc. La charge corporelle maximum etait de 1340 nc. Le memoire presente des details sur les variations des charges de radiocesium chez l'homme selon l'age, le sexe, l'occupation et le regime alimentaire, ainsi que sur les fluctuations saisonnieres et geographiques qui ont ete relevees. On a observe un accroissement de la charge corporelle moyenne de cesium 137 de 1961 a 1963. Aux fins de comparaison, on a egalement fait des dosages sur des habitants des memes regions autres que lapons. Chez presque tous, la charge de cesium 137 etait sensiblement superieure a celle quia ete trouvee chez les habitants de la Suede meridionale. La dose de rayonnement due au radiocesium est, dans bien des cas.de deux a trois fois la dose qui provient du potassium 40, le maximum observe etant de huit fois cette dose. (author) [Spanish] Despues de descubrirse a principios de 1961 ia elevada carga corporal de cesio-137 que tenfan los lapones suecos, se han efectuado mediciones en grandes grupos de lapones y otros habitantes de Suecia que siguen un regimen 'alimenticio' analogo. En septiembre de 1961 se examinaron 162 personas.de una zona proxima al Circulo polar artico con un'antropogammametro semiportatil. En los lapones se registraron cargas de cesio-137 comprendidas entre 15 y 690 nc. En la misma region, se sometio a

  13. Fatores de risco associados à pressão arterial elevada em adolescentes Risk factors associated with high blood pressure in adolescents

    Directory of Open Access Journals (Sweden)

    Kelly Samara da Silva

    2007-08-01

    Full Text Available Níveis elevados de pressão arterial (PA na infância e adolescência têm sido freqüentemente associados a outros fatores de risco cardiovasculares, além de hipertensão arterial na fase adulta da vida. O presente estudo objetivou identificar fatores de risco associados à PA elevada em adolescentes. Participaram do estudo 674 adolescentes de escolas públicas e privadas, de ambos os sexos (303 rapazes e 371 moças, com idade de 14 a 17 anos (16,5 DP 0,9, da cidade de João Pessoa, Paraíba. Mediante aplicação de um questionário, foram levantadas informações sobre: a indicadores sociodemográficos; b nível de prática da atividade física; c hábitos alimentares; c índice de massa corporal; e fumo e bebidas alcoólicas; e foram realizadas medidas de PA. A prevalência de PA elevada foi de 7,4%, sendo maior nos rapazes (10,2% quando comparada com a das moças (5,1%, p High levels of arterial blood pressure in childhood and adolescence have been often associated with other cardiovascular risk factors apart from arterial hypertension in the adult stage of life. The present study aimed at identifying risk factors associated with high blood pressure in adolescents. The subjects were 674 adolescents from public and private schools in the city of João Pessoa, Paraíba. These subjects were of both sexes (i.e., 303 boys and 371 girls and their ages spanned from 14 to 17 years old. By means of a questionnaire, information about the following aspects was gathered: a socio-demographic status; b physical activity levels; c eating habits; d body mass index; e consumption of alcohol and smoking. Moreover, measurements of arterial pressure (AP showed that the prevalence of high AP was 7.4 %, being higher in boys (10.2% when compared with girls (5.1%, p < 0.01. The results of the multivaried analysis indicated that overweight adolescents displayed from five to six times more chance of having high AP than adolescents with light/normal weight (boys: OR

  14. Avaliação do tipo de agregado e da reidratação Do concreto submetido à elevadas temperaturas

    Directory of Open Access Journals (Sweden)

    A. A. A. de Souza

    Full Text Available O efeito do comportamento do concreto perante o fogo está sendo estudado por um grupo formado no Laboratório de Estruturas e Construção Civil da Faculdade de Engenharia Civil, Arquitetura e Urbanismo da UNICAMP. Este trabalho apresenta uma investigação sobre a perda de re- sistência mecânica ocorrida em diferentes misturas de concreto depois de submetidas a temperaturas elevadas, bem como, uma investigação da influência da reidratação. As misturas foram preparadas com três diferentes agregados: argila expandida, basalto e calcário. Os corpos-de-prova C, obedecendo a mesma taxa de 300 ºC e 600 ºC foram submetidos às temperaturas de 300 aquecimento da Curva ISO-834 (1999. Após o trata- mento térmico, parte dos corpos-de-prova foram reidratados ao ar, outra parte foi imersa em água e outros envolvidos em filme plástico durante 28, 56 e 112 dias. Nestas idades os concretos foram caracterizados em relação à resistência à compressão, resistência à tração e módulo de elasticidade e assim uma avaliação da influência do tipo de agregado e da reidratação na recuperação da resistência mecânica pôde ser feita.

  15. Quantitation of IgE by means of a modified radial immunodiffusion method in comparison with the radioimmunosorbent test (RIST)

    International Nuclear Information System (INIS)

    Wiedermann, G.; Stemberg, H.; Kraft, D.; Ambrosch, F.; Schadlbauer, B.; Jarischko, E.; Vienna Univ.

    1974-01-01

    Serum IgE were quantitied by means of modified radial immunodiffusion technique (RID). To improve visibility of precipitin bands a staining procedure with DOPA was applied. Pretreatment of sera with dextransulfate proved necessary in order to avoid unspecific ringformation in the agargel. In comparison with the RIST it turned out that sera containing less than 500 I.U. IgE/ml did not produce precipitin bands with this method. Sera containing 500-999 I.U. IgE/ml occasionally exhibited positive results with the RID technique, whereas sera with more than 1,000 I.U./ml were regulary positive. In its present form the RID may be used as screening method for sera with higher IgE levels. Within the above mentioned limits the IgE levels calculated by means of the RID-test roughly corresponded the values determined by the RIST. (orig.) [de

  16. Relationship between specific IgE to egg components and natural history of egg allergy in Danish children

    DEFF Research Database (Denmark)

    Gradman, Josefine; Mortz, Charlotte G; Eller, Esben

    2016-01-01

    BACKGROUND: The majority of egg-allergic children develop tolerance over time. However, it may take numerous of consecutive egg challenges to get there as no good indices to predict tolerance exist. OBJECTIVE: To investigate whether serial measurements of specific IgE to egg white, ovomucoid......, ovalbumin, conalbumin, lysozyme, and egg yolk could improve the specificity of the diagnostic workup and aid in the decision when to rechallenge egg-allergic children. METHODS: The outcome of oral food challenges with hen's egg and corresponding specific IgE levels were evaluated in children referred...... to The Allergy Center within an 8-year period. The egg-allergic children were rechallenged and had specific IgE levels measured once a year. RESULTS: During a median follow-up of 26 months, 287 challenges and corresponding 287 serum analyses were performed in 130 children. Of the 130 children, 99 were egg...

  17. Tus-Ter-lock immuno-PCR assays for the sensitive detection of tropomyosin-specific IgE antibodies.

    Science.gov (United States)

    Johnston, Elecia B; Kamath, Sandip D; Lopata, Andreas L; Schaeffer, Patrick M

    2014-02-01

    The increasing prevalence of food allergies requires development of specific and sensitive tests capable of identifying the allergen responsible for the disease. The development of serologic tests that can detect specific IgE antibodies to allergenic proteins would, therefore, be highly received. Here we present two new quantitative immuno-PCR assays for the sensitive detection of antibodies specific to the shrimp allergen tropomyosin. Both assays are based on the self-assembling Tus-Ter-lock protein-DNA conjugation system. Significantly elevated levels of tropomyosin-specific IgE were detected in sera from patients allergic to shrimp. This is the first time an allergenic protein has been fused with Tus to enable specific IgE antibody detection in human sera by quantitative immuno-PCR.

  18. Pyoderma gangrenosum in association with microscopic colitis, idiopathic hypereosinophilic syndrome, selective IgE deficiency and diabetes mellitus.

    Science.gov (United States)

    Riyaz, N; Sasidharanpillai, S; Rahima, S; Bindu, V; Shaan, M; Raghavan, N T; Mohan, L; Janardhanan, A K

    2015-08-01

    Pyoderma gangrenosum (PG) is a neutrophilic dermatosis of unknown aetiology. We report a 27-year-old male patient with diabetes, who presented with a nonhealing ulcer on the left leg, pruritic hyperpigmented papules distributed over the trunk and limbs, and chronic diarrhoea. He had eosinophilia, low haemoglobin and serum IgE levels, and raised erythrocyte sedimentation rate. Histopathology of the leg ulcer was consistent with the diagnosis of PG, while the histology of the hyperpigmented papule revealed tissue eosinophilia. Subsequent evaluation was conclusive of the diagnosis of PG, idiopathic hypereosinophilic syndrome (IHES) and selective IgE deficiency. Dexamethasone pulse therapy achieved resolution of the ulcer and reduction in the eosinophilia. Further evaluation for the persistent diarrhoea led to a diagnosis of lymphocytic colitis (LC), which responded to budesonide. To our knowledge, the association of PG with IHES, selective IgE deficiency or LC has not been previously reported. © 2015 British Association of Dermatologists.

  19. Heat-induced alterations in cashew allergen solubility and IgE binding

    Directory of Open Access Journals (Sweden)

    Christopher P. Mattison

    Full Text Available Cashew nuts are an increasingly common cause of food allergy. We compare the soluble protein profile of cashew nuts following heating. SDS-PAGE indicate that heating can alter the solubility of cashew nut proteins. The 11S legumin, Ana o 2, dominates the soluble protein content in ready to eat and mildly heated cashew nuts. However, we found that in dark-roasted cashew nuts, the soluble protein profile shifts and the 2S albumin Ana o 3 composes up to 40% of the soluble protein. Analysis of trypsin-treated extracts by LC/MS/MS indicate changes in the relative number and intensity of peptides. The relative cumulative intensity of the 5 most commonly observed Ana o 1 and 2 peptides are altered by heating, while those of the 5 most commonly observed Ana o 3 peptides remaine relatively constant. ELISA experiments indicate that there is a decrease in rabbit IgG and human serum IgE binding to soluble cashew proteins following heating. Our findings indicate that heating can alter the solubility of cashew allergens, resulting in altered IgE binding. Our results support the use of both Ana o 2 and Ana o 3 as potential cashew allergen diagnostic targets. Keywords: Cashew nut, Food allergy, Immunoglobulin E, Mass-spectrometry, Peptide, Solubility

  20. IgE sensitization and sociodemographic conditions as determinant factors in asthma severity

    Directory of Open Access Journals (Sweden)

    Rodrigo Gaviria

    2017-12-01

    Full Text Available Background: In Latin America there are few data about the factors that influence the control and severity of asthma. Objective: To describe the association between IgE sensitization to intra-home allergens and housing conditions in the severity of asthma. Methods: Sensitization to aero-allergens was evaluated by skin prick test and socio-demographic data by a questionnaire in a population between 6 and 14 years of age with diagnosis of asthma. Asthma control was measured according to spirometric results and to the application of the ACT (Asthma Control Test; the severity and symptom control was evaluated according to the GINA guideline. Results: A total of 150 resident patients from the Aburra Valley (Colombia were included. The median age of participants was 11 years, 95 (63.3 % male. 92 % of the patients resided in the urban area. Mite sensitization was the most prevalent in controlled and uncontrolled patients. Sensitization to cockroach and some poverty conditions were risk factors for asthma severity. Conclusion: Poverty conditions appear to favor the development of severe asthma and in turn IgE sensitization to cockroaches. This sensitization could serve as a biomarker of severity.

  1. Negativization rates of IgE radioimmunoassay and basophil activation test in immediate reactions to penicillins.

    Science.gov (United States)

    Fernández, T D; Torres, M J; Blanca-López, N; Rodríguez-Bada, J L; Gomez, E; Canto, G; Mayorga, C; Blanca, M

    2009-02-01

    Skin test sensitivity in patients with immediate allergy to penicillins tends to decrease over time, but no information is available concerning in vitro tests. We analysed the negativization rates of two in vitro methods that determine specific immunoglobulin E (IgE) antibodies, the basophil activation test using flow cytometry (BAT) and the radioallergosorbent test (RAST), in immediate allergic reactions to penicillins. Forty-one patients with immediate allergic reactions to amoxicillin were followed up over a 4-year period. BAT and RAST were performed at 6-month intervals. Patients were randomized into groups: Group I, skin tests carried out at regular intervals; Group II, skin tests made only at the beginning of the study. Differences were observed between RAST and BAT (P testing influenced the rate of negativization of the RAST assay, contributing to maintenance of in vitro sensitivity. Because of the loss of sensitivity over time, the determination of specific IgE antibodies to penicillins in patients with immediate allergic reactions must be done as soon as possible after the reaction.

  2. Prevalence of IgE sensitization in Danish children with suspected asthma

    DEFF Research Database (Denmark)

    Hoffmann-Petersen, Benjamin; Høst, Arne; Larsen, Kirsten Toksvig

    2013-01-01

    OBJECTIVE: The aim of this article was to estimate the prevalence of IgE sensitization in Danish children with suspected asthma and to characterize the pattern of sensitization. STUDY DESIGN: We performed a cross-sectional study including 1744 children from 0 to 15 yr suspected of asthma who were...... referred to pediatric outpatient clinics in the region of southern Denmark from 2003 to 2005. The children were subjected to an extensive questionnaire-based interview, clinical examination, and both skin prick testing (SPT) and IgE measurements for 17 allergens. RESULTS: Asthma was confirmed in 1024...... of the 1744 children. Among the children in whom the asthma diagnosis was confirmed, sensitization to one or more of the 17 allergens tested was found in 67.5% by either SPT or s-IgE ≥ class 2. Sensitization to any food allergen was found in 31.1%, to any outdoor allergen in 36.2%, and to any indoor allergen...

  3. IgE and risk of cancer in 37 747 individuals from the general population

    DEFF Research Database (Denmark)

    Helby, J; Bojesen, S E; Nielsen, S F

    2015-01-01

    E are associated with overall risk of cancer and with risk of specific cancers. MATERIALS AND METHODS: Plasma total IgE was measured in 37 747 individuals from the general population, and the participants were followed prospectively for up to 30 years. All statistical tests were two-sided. RESULTS: During a mean...... follow-up of 7 years, a first cancer was diagnosed in 3454 participants. The multivariable adjusted hazard ratio for a 10-fold higher level of IgE was 1.05 [95% confidence interval (CI) 1.00-1.11; P = 0.04] for any cancer, 0.44 (0.30-0.64; P = 0.00002) for chronic lymphocytic leukemia (CLL), 0.53 (0.......33-0.84; P = 0.007) for multiple myeloma, 1.54 (1.04-2.29; P = 0.03) for other non-Hodgkin lymphoma, 1.38 (1.04-1.84; P = 0.03) for cancer of the oral cavity and pharynx, and 1.12 (1.00-1.25; P = 0.05) for lung cancer. The findings for CLL and multiple myeloma were generally robust; however, after correcting...

  4. IgE and the role of the Radio-Allergo-Sorbent Test (RAST)

    International Nuclear Information System (INIS)

    Grond, K.

    1976-01-01

    In 1966 a new immunoglobulin was found in persons with allergies and in non-typical myeloma proteins. Normally this immunoglobulin E is present only in nanogramms and rests with predilection on the membrane of mast-cells. There is a reaginic-anaphylactic reaction after re-exposure of antigens to the antigen-antibody reaction followed by denudation of mediators of the anaphylactic reaction. With the Radio-Immuno-Sorbent-Test (RIST) the IgE can be quantitatively determined. Elevated IgE-blood levels are typically found in atopic eczema. With the Radio-Allergo-Sorbent-Test (RAST) the allergen specific IgE can be defined. A conformity with appropriate patchtests can be achieved in 60-80% of the cases. In this review advantoses and problem of RIST- and RAST-diagnoses are described. RAST presents a valuable aid in diagnosis of allergies beeing not burdensome and risky, as it is easy to perform and bears no risk to the patients. At the present time, however, patch tests are necessary in the diagnosis of allergies. (orig.) [de

  5. Aspectos epidemiológicos dos pólipos e lesões plano-elevadas colorretais Epidemiological aspects of colorectal polyps and flat adenomas

    Directory of Open Access Journals (Sweden)

    Juvenal da Rocha Torres Neto

    2010-12-01

    Full Text Available INTRODUÇÃO: Os pólipos e as lesões plano-elevadas colorretais são importantes na prevenção do câncer colorretal pelo risco de malignização dos adenomas. OBJETIVO: traçar o perfil demográfico dos pacientes com diagnóstico endoscópico de pólipos e/ou lesões plano-elevadas colorretais no Hospital Universitário da Universidade Federal de Sergipe e Torres Centro Médico. MÉTODOS: Foram avaliados 6.919 prontuários de 2002 a 2007, enfatizando-se as variáveis: idade, gênero, procedência e indicação; número, tamanho, morfologia, histologia, grau de displasia, topografia, lesões sincrônicas e metacrônicas, e diagnósticos associados. RESULTADOS: Foram encontradas 1.031 (13,51% lesões em 935 exames, correspondendo a 826 pacientes, 46% masculino e 54% feminino. A idade variou de 3 a 96 anos, com média de 53,64. A distribuição topográfica mais frequente das lesões polipoides foi em reto e sigmoide (58,40%. As lesões eram sésseis em 52,80%, pediculadas em 27,90% e plano-elevadas em 19,30% dos casos. Ocorreram lesões sincrônicas em 23,48% e metacrônicas em 30,10% dos pacientes. Histologicamente, 43,36% eram adenomas, sendo 85,70% tubulares, 9,60% tubulo-vilosos e 4,70% vilosos; 30,64% eram pólipos hiperplásicos, 15,80% inflamatórios e 10,20% possuíam outros tipos histológicos. Os adenomas apresentavam displasia de baixo grau em 83,40% dos casos e alto grau em 16,60%. Sete eram adenocarcinomas, um carcinoide e um tumor gastrointestinal estromal. CONCLUSÕES: A colonoscopia e a polipectomia são importantes no diagnóstico e prevenção do câncer colorretal.INTRODUCTION: Colorectal polyps and flat injuries are important for the prevention of colorectal cancer due to the malignancy of adenomas. OBJECTIVE: To describe the demographic profile of patients with endoscopic diagnosis of colorectal polyps and/or flat injuries at University Hospital of Universidade Federal de Sergipe and Torres Medical Center. METHODS: A total

  6. Effect of in vitro irradiation and cell cycle-inhibitory drugs on the spontaneous human IgE synthesis in vitro

    International Nuclear Information System (INIS)

    Del Prete, G.F.; Vercelli, D.; Tiri, A.; Maggi, E.; Rossi, O.; Romagnani, S.; Ricci, M.

    1987-01-01

    The in vitro effects of radiation, diterpine forskolin (FK), and hydrocortisone (HC) on the in vitro spontaneous IgE synthesis by peripheral blood B-lymphocytes from atopic patients were investigated. Without affecting cell viability, in vitro irradiation inhibited in a dose-dependent fashion de novo IgE synthesis in vitro by B cells from all patients examined with a mean 40% reduction of in vitro IgE product after treatment with 100 rads. In contrast, the in vitro IgE production by the U266 myeloma cell line was unaffected, even by irradiation with 1600 rads. The addition to B cell cultures from atopic patients of FK consistently resulted in a dose-dependent inhibition of the spontaneous IgE production in vitro. The addition to cultures of 10(-5) and 10(-6) molar concentrations of HC was also usually inhibitory, whereas lower HC concentrations were uneffective or even enhanced the spontaneous in vitro IgE synthesis. When 10(-6) molar concentrations of both HC and FK were combined in culture, a summation inhibitory effect on the spontaneous IgE synthesis was observed. In contrast, neither FK nor HC had inhibitory effect on the in vitro spontaneous IgE synthesis by the U266 myeloma cell line. The spontaneous in vitro IgE synthesis by B cells from patients with Hodgkin's disease, demonstrating high levels of serum IgE, was strongly reduced or virtually abolished after patients underwent total nodal irradiation to prevent the spread of the disease. In addition, the in vitro spontaneous IgE synthesis by B cells from atopic patients was markedly decreased or abolished by in vivo administration of betamethasone

  7. IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.

    Directory of Open Access Journals (Sweden)

    Aleksandra Słodka

    Full Text Available BACKGROUND: Immunoglobulin E (IgE binds to high affinity receptor FcεRI numerously expressed on mast cells. Recent findings have revealed that IgE by itself may regulate various aspects of mast cell biology, however, detailed data is still limited. METHODOLOGY/FINDINGS: Here, we have examined the influence of IgE alone, used at different concentrations, on mast cell activity and releasability. For the study we have employed in vivo differentiated mature tissue mast cells isolated from rat peritoneal cavity. Mast cells were exposed to IgE alone and then the release of preformed and de novo-synthesized mediators, surface FcεRI expression and mast cell migratory response were assessed. IgE by itself was found to up-regulate FcεRI expression and activate mast cells to degranulation, as well as de novo synthesis and release of cysteinyl leukotrienes and TNF. We have provided evidence that IgE alone also amplified spontaneous and CCL5- or TNF-induced migration of mast cells. Importantly, IgE was effective only at concentrations ≥ 3 µg/mL. A molecular basis investigation using an array of specific inhibitors showed that Src kinases, PLC/PLA2, MAP kinases (ERK and p38 and PI3K were entirely or partially involved in IgE-induced mast cell response. Furthermore, IgE alone stimulated the phosphorylation of MAP kinases and PI3K in rat mast cells. CONCLUSION: Our results clearly demonstrated that IgE by itself, at higher concentrations, influences mast cell activity and releasability. As there are different conditions when the IgE level is raised it might be supposed that in vivo IgE is one of the important factors modulating mast cell biology within tissues.

  8. Utility of serum periostin and free IgE levels in evaluating responsiveness to omalizumab in patients with severe asthma.

    Science.gov (United States)

    Tajiri, T; Matsumoto, H; Gon, Y; Ito, R; Hashimoto, S; Izuhara, K; Suzukawa, M; Ohta, K; Ono, J; Ohta, S; Ito, I; Oguma, T; Inoue, H; Iwata, T; Kanemitsu, Y; Nagasaki, T; Niimi, A; Mishima, M

    2016-10-01

    Omalizumab, a humanized anti-IgE monoclonal antibody, has demonstrated efficacy in patients with severe allergic asthma. However, treatment responses vary widely among individuals. Despite a lack of data, free serum IgE levels following omalizumab treatment have been proposed as a marker of treatment responsiveness. In this prospective, observational study, we assessed the utility of biomarkers of type 2 inflammation in predicting omalizumab treatment responses, as determined by the absence of asthma exacerbation during the first year of treatment. Free serum IgE levels were monitored for 2 years to examine their association with baseline biomarker levels and the number of exacerbations. We enrolled thirty patients who had been treated with omalizumab for at least 1 year, of whom 27 were treated for 2 years. Baseline serum periostin levels and blood eosinophil counts were significantly higher in patients without exacerbations during the first year of treatment than in patients with exacerbations. Baseline serum periostin levels, but not eosinophil counts, were negatively associated with free serum IgE levels after 16 or 32 weeks of treatment. Reduced free serum IgE levels during treatment from those at baseline were associated with reduced exacerbation numbers at 2 years. In 14 patients who continued to have exacerbations during the first year of treatment, exacerbation numbers gradually and significantly decreased over the 2-year study period, with concurrent significant reductions in free serum IgE levels. Baseline serum periostin levels and serum free IgE levels during treatment follow-up may be useful in evaluating responses to omalizumab treatment. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  9. The correlation between anti phospholipase A 2 specific IgE and clinical symptoms after a bee sting in beekeepers

    Directory of Open Access Journals (Sweden)

    Jan Matysiak

    2016-06-01

    Full Text Available Introduction: Beekeepers are a group of people with high exposure to honeybee stings and with a very high risk of allergy to bee venom. Therefore, they are a proper population to study the correlations between clinical symptoms and results of diagnostic tests. Aim: The primary aim of our study was to assess the correlations between total IgE, venom- and phospholipase A 2 -specific IgE and clinical symptoms after a bee sting in beekeepers. The secondary aim was to compare the results of diagnostic tests in beekeepers and in individuals with standard exposure to bees. Material and methods: Fifty-four individuals were divided into two groups: beekeepers and control group. The levels of total IgE (tIgE, venom-specific IgE (venom sIgE, and phospholipase A 2 -specific IgE (phospholipase A 2 sIgE were analyzed. Results: Our study showed no statistically significant correlation between the clinical symptoms after a sting and tIgE in the entire analyzed group. There was also no correlation between venom sIgE level and clinical symptoms either in beekeepers or in the group with standard exposure to bees. We observed a statistically significant correlation between phospholipase A 2 sIgE level and clinical signs after a sting in the group of beekeepers, whereas no such correlation was detected in the control group. Significantly higher venom-specific IgE levels in the beekeepers, as compared to control individuals were shown. Conclusions : In beekeepers, the severity of clinical symptoms after a bee sting correlated better with phospholipase A 2 sIgE than with venom sIgE levels.

  10. Determination of allergen specificity by heavy chains in grass pollen allergen-specific IgE antibodies.

    Science.gov (United States)

    Gadermaier, Elisabeth; Flicker, Sabine; Lupinek, Christian; Steinberger, Peter; Valenta, Rudolf

    2013-04-01

    Affinity and clonality of allergen-specific IgE antibodies are important determinants for the magnitude of IgE-mediated allergic inflammation. We sought to analyze the contribution of heavy and light chains of human allergen-specific IgE antibodies for allergen specificity and to test whether promiscuous pairing of heavy and light chains with different allergen specificity allows binding and might affect affinity. Ten IgE Fabs specific for 3 non-cross-reactive major timothy grass pollen allergens (Phl p 1, Phl p 2, and Phl p 5) obtained by means of combinatorial cloning from patients with grass pollen allergy were used to construct stable recombinant single chain variable fragments (ScFvs) representing the original Fabs and shuffled ScFvs in which heavy chains were recombined with light chains from IgE Fabs with specificity for other allergens by using the pCANTAB 5 E expression system. Possible ancestor genes for the heavy chain and light chain variable region-encoding genes were determined by using sequence comparison with the ImMunoGeneTics database, and their chromosomal locations were determined. Recombinant ScFvs were tested for allergen specificity and epitope recognition by means of direct and sandwich ELISA, and affinity by using surface plasmon resonance experiments. The shuffling experiments demonstrate that promiscuous pairing of heavy and light chains is possible and maintains allergen specificity, which is mainly determined by the heavy chains. ScFvs consisting of different heavy and light chains exhibited different affinities and even epitope specificity for the corresponding allergen. Our results indicate that allergen specificity of allergen-specific IgE is mainly determined by the heavy chains. Different heavy and light chain pairings in allergen-specific IgE antibodies affect affinity and epitope specificity and thus might influence clinical reactivity to allergens. Copyright © 2012 American Academy of Allergy, Asthma & Immunology. Published by

  11. Presence of functional, autoreactive human milk-specific IgE in infants with cow's milk allergy.

    Science.gov (United States)

    Järvinen, K M; Geller, L; Bencharitiwong, R; Sampson, H A

    2012-02-01

    Occasionally, exclusively breastfed infants with cow's milk allergy (CMA) remain symptomatic despite strict maternal milk avoidance. To determine whether or not persistence of symptoms could be due to sensitization against endogenous human milk proteins with a high degree of similarity to bovine allergens. Ten peptides representing known bovine milk IgE-binding epitopes [α-lactalbumin (ALA), β- and κ-casein] and the corresponding, highly homologous human milk peptides were labelled with sera from 15 breastfed infants with CMA, aged 3 weeks to 12 months, and peptide (epitope)-specific IgE antibodies were assessed. Nine of the 15 breastfed infants became asymptomatic during strict maternal avoidance of milk and other major food allergens; six infants remained symptomatic until weaned. Ten older children, aged 5-15 years, with CMA were also assessed. The functional capacity of specific IgE antibodies was assessed by measuring β-hexosaminidase release from rat basophilic leukaemia cells passively sensitized and stimulated with human and bovine ALA. A minimum of one human milk peptide was recognized by IgE antibodies from 9 of 15 (60%) milk-allergic infants, and the majority of older children with CMA. Genuine sensitization to human milk peptides in the absence of IgE to bovine milk was occasionally seen. There was a trend towards specific IgE being detected to more human milk peptides in those infants who did not respond to the maternal milk elimination diet than in those who did (P = 0.099). Functional IgE antibody to human ALA was only detected in infants not responding to the maternal diet. Endogenous human milk epitopes are recognized by specific IgE from the majority of infants and children with CMA. Such autoreactive, human milk-specific IgE antibodies appear to have functional properties in vitro. Their role in provoking allergic symptoms in infants exclusively breastfed by mothers strictly avoiding dietary milk remains unclear. © 2011 Blackwell

  12. Influence of non-metallic second phases on fatigue behaviour of high strength steel components; Efecto de segundas fases no metalicas sobre el comportamiento a fatiga de componentes de acero con elevadas solicitaciones

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, L.; Elvira, R.; Garcia de Andoin, A.; Pizarro, R.; Bertrand, C.

    2005-07-01

    To assess the real effect of the inclusion type on fatigue life of ultra clean high strength steels mechanical components made of 100Cr6 steel were fatigue tested and fracture surfaces analysed to determine the origin of fatigue cracks.Two heats proceedings from different steelmaking routes were taken for the tests. The material were forged into ring shape components which were fatigue tested under compression-compression loads. Failures were analysed by SFEM (Scanning field Emission Microscopy), proving that most of failures at high loads were originated by manganese sulphides of small size (10-70 micros), while less than 40% of all fatigue cracks due to inclusions were caused by titanium carbonitrides and hard oxides. It has been demonstrated that once number and size of hard inclusions have been reduced, the hazardous effect of oxides and carbonitrides on the fatigue life decreases also. However, softer inclusions as manganese sulphides, currently considered as less hazardous, play a more relevant role as direct cause of fatigue failure and they should be taken into account in a deeper way in order to balance both machinability and fatigue life requirements in high strength steel components. (Author) 11 refs.

  13. Estandarización de la TR-PCR para la detección de las fusiones génicas BCR-ABL en pacientes con leucemia Mieloide Crónica (LMC y Linfoide Aguda (LLA provenientes de HUSVP y Clíncia León XIII

    Directory of Open Access Journals (Sweden)

    Gonzálo Vásquez Palacio

    2006-04-01

    Full Text Available La translocación recíproca t(9:22(q34;q11 involucra el proto-oncogen ABL y el gen BCR, originando un gen de fusión BCR-ABL, que codifica una proteína con elevada actividad tirosina quinasa, implicada en la leucemogénesis.

  14. Modulation of total IgE levels in serum of normal and athymic nude BALB/c mice by cells and exogenous antigenic stimulation

    NARCIS (Netherlands)

    Savelkoul, H.F.J.; Akker, van den T.W.; Soeting, P.W.C.; Oudenaren, van A.; Benner, R.

    1989-01-01

    Several different grades of T-system impairment were studied for their effects on the total serum IgE concentration in BALB/c mice. Homozygous athymic nu/nu mice and their heterozygous nu/ littermates were compared for serum IgE levels while kept under either barrier-maintained or conventional

  15. The pharmacological mechanisms of omalizumab in patients with very high IgE levels—Clues from studies on atopic dermatitis

    Directory of Open Access Journals (Sweden)

    Tse Wen Chang

    2012-12-01

    Full Text Available Seventeen case series investigating the effects of omalizumab on patients with atopic dermatitis included patients whose pretreatment serum IgE was above 700 IU/ml, the upper inclusion limit specified in the product label. In all, 107 patients received omalizumab at doses of ≤375 mg every 2 weeks, which is recommended for patients with IgE <700 IU/ml. Among them, 87 improved in clinical symptoms and some did so after the first dose. Among these 87 patients, 35 and 12 had pretreatment serum IgE in the range 700–7000 IU/ml and 7000–121,000 IU/ml, respectively. These results not only suggest the pathogenic roles of IgE and the potential utility of omalizumab in atopic dermatitis, but also raise questions concerning the pharmacological mechanisms of omalizumab in patients with very high IgE levels. If omalizumab at regular doses is proven to treat patients with ultra high IgE (e.g. above 7000 IU/ml effectively, it probably achieves this without neutralizing most of the IgE produced in the patients and downregulating the high-affinity IgE-Fc receptors on basophils and mast cells. Herein, we propose that a potential main pharmacological mechanism of omalizumab in patients with ultra high IgE is the ability of the rapidly accumulated IgE:omalizumab complexes to trap allergens.

  16. Postmortem serum levels of tryptase and total and specific IgE in fatal asthma.

    Science.gov (United States)

    Salkie, M L; Mitchell, I; Revers, C W; Karkhanis, A; Butt, J; Tough, S; Green, F H

    1998-01-01

    Sera were obtained postmortem from 55 subjects classified into three groups; death due to asthma (FA, n = 21), asthmatic but death not due to asthma (NFA, n = 24) and a nonasthmatic control group (NAC, n = 10). A full autopsy was performed on all cases and a medical history, including details of allergies, was obtained by questionnaire from the next of kin. Grading of asthma severity by either questionnaire or autopsy was comparable (tP = 0.435, p > 0.05) and the mean pathology-grade was significantly higher for the FA group (3.375) compared to the NFA group (2.375), p 2.0 micrograms/L) in 21/55 sera (38%) and there was no significant difference between the groups. ROC plots showed that tryptase levels did not discriminate between the FA and NFA groups, even if specimens were collected within 24 hours after death. Total IgE was significantly elevated in the FA group (geometric mean 140.3 kU/L) compared to the other two groups (NFA 30.2 kU/L, NAC 9.4 kU/L), p = 0.05. Fatal asthmatics also had a greater positivity (67%) to a screen for common inhalant allergens than did the other groups (NFA 30%, NAC 20%). Sera with a positive screen were tested against a panel of 10 common aero-allergens. Each sample was then assigned a number (N) and a score (S), dependent on either the number of allergens positive (N) or the total sum of pluses for all allergens (S). Both the N and S values were higher for the FA group (N = 98, S = 264) than the NFA group (N = 52, S = 151) and NAC group (N = 4, S = 8). The ratio (S/N) which gives an index (I) was 2.69, 2.90, and 2.00, respectively. Tryptase was poorly correlated to the total IgE level (r = 0.036); however, mean values for N and S were significantly different (N 6.81, S 4.50, and N 19.25, S 11.5, p or = 2.0 micrograms/L, respectively. We conclude that total and specific IgE may be useful predictors of asthma severity but that postmortem tryptase is not useful in the diagnosis of a fatal asthmatic attack.

  17. GAMBARAN IgG4 dan IgE TERHADAP PROTEIN MIKROFILARIA PADA SERA PENDUDUK ENDEMIS FILARIASIS DI KECAMATAN PASIR PENYU, RIAU

    Directory of Open Access Journals (Sweden)

    Basundari SU

    2012-09-01

    Full Text Available Western blot test to detect specific IgG4 and IgE was performed to 12 microfilaraemic and 13 amicrofilaraemic individuals from malayan filariasis endemic area, Pasir Penyu, Riau. No differences in binding patterns of IgG4 and IgE antibodies to microfUarial protein components was shown. There was a parallel protein components recognition by IgG4 and IgE of molecular weight ranging from 158 kd to 14 kd. Protein component of 125 kd was only recognized by IgG4 and of 112 kd only by IgE. These findings suggest that in filarial infection IgG4 antibodies play a role as a blocking antibodies to inhibit the spesific reaction of IgE that is usually expressed as an allergic reaction.

  18. Epidemiologia das pregas cutâneas triciptal e subescapular elevadas em adolescentes Epidemiology of elevated triciptal and subscapular skinfolds in adolescents

    Directory of Open Access Journals (Sweden)

    Rodrigo Pereira Duquia

    2008-01-01

    Full Text Available O objetivo deste estudo foi descrever e comparar as pregas cutâneas triciptal (PCT e subescapular (PCS de acordo com características demográficas, sócio-econômicas, comportamentais e biológicas em adolescentes de Pelotas, Rio Grande do Sul, Brasil. Realizou-se um estudo transversal aninhado a uma coorte de nascimentos (N = 4.452; média de idade = 11 anos. Os desfechos foram apresentar prega cutânea maior ou igual ao percentil 90 da curva de referência do National Center for Health Statistics. A prevalência de PCT e PCS elevada foi, respectivamente, de 20,2% e 17,3% nos meninos e de 14,2% e 10,5% nas meninas. O fator mais fortemente associado com adiposidade nos meninos foi o nível econômico (p The objective of this study was to describe and compare the tricipital (TSF and subscapular skinfold (SSF thickness according to demographic, socioeconomic, behavioral, and biological characteristics in adolescents from Pelotas, Rio Grande do Sul State, Brazil. A cross-sectional study nested in a birth cohort (n = 4,452; mean age = 11 years was conducted. The outcomes were defined as skinfold thickness > 90th percentile of the National Center for Health Statistics reference curve. The prevalence rates for elevated TSF and SSF were 20.2% and 17.3%, respectively, in boys and 14.2% and 10.5% in girls. The strongest factor associated with adiposity in boys was socioeconomic status (p < 0.001, whereas among girls it was maternal body mass index (p < 0.001. Low physical activity (< 300 minutes/week was associated with elevated SSF only among girls, while schooling was associated with increased TSF and SSF only among boys. Diet, skin color, and sedentary behavior were not associated with any of the outcomes. We concluded that the main predictors of adiposity were maternal and socioeconomic characteristics. We recommend that further studies on this issue apply other methods to estimate body composition in order to confirm our results.

  19. Efeito de elevadas produtividades do vinhedo nas características físico-químicas e sensoriais do vinho Merlot

    Directory of Open Access Journals (Sweden)

    Miele Alberto

    2006-01-01

    Full Text Available O objetivo do trabalho foi determinar o efeito de elevadas produtividades dos vinhedos nas características físico-químicas e sensoriais do vinho Merlot jovem produzido em região de montanha. O experimento foi realizado durante o ciclo vegetativo da videira de 1999/2000, constando de quatro tratamentos (produtividades do vinhedo - kg ha-1 - 22.400, 27.800, 34.700 e 44.900 e três repetições; cada parcela foi constituída de cinco plantas. A uva foi colhida em fevereiro de 2000, por ocasião de sua maturação, e levada ao Laboratório de Microvinificação onde foi vinificada em recipientes de vidro de 20L. Avaliaram-se as variáveis físico-químicas por processos clássicos e as sensoriais por um grupo de degustadores utilizando-se uma ficha de degustação não-paramétrica. Os dados obtidos foram submetidos à análise de regressão polinomial. Os resultados mostram que a produtividade crescente do vinhedo não teve efeito significativo sobre as variáveis físico-químicas. Entretanto ela foi significativa sobre as variáveis visuais, olfativas e gustativas. Os vinhos elaborados com uvas provenientes de vinhedos com a menor produtividade apresentaram-se visualmente com matiz vermelho-violáceo mais acentuado; mais francos, finos e típicos no olfato; e com gosto mais equilibrado, típicos, frutados e mais encorpados. Os vinhos de média produtividade tiveram cor mais intensa; olfato também mais intenso, mas com notas vegetais e de pimentão verde mais acentuadas; gosto mais intenso. As demais variáveis sensoriais não foram afetadas pela produtividade do vinhedo.

  20. The quantitation of parasite-specific human IgG and IgE in sera: evaluation of solid-phase RIA and ELISA methodology

    International Nuclear Information System (INIS)

    Hamilton, R.G.; Adkinson, N.F. Jr.

    1981-01-01

    The authors have developed a non-competitive solid-phase radioimmunoassay (SPRIA) to quantitate both human IgE and IgG antibodies against soluble adult antigens of Brugia malayi (B.m.), a filarial parasite causing extensive infection throughout the tropics. Previously enzyme-linked immunosorbent assays (ELISA) had been used to detect μg/ml levels of IgG anti-B.m., but IgE antibodies were difficult to detect in this system. Since the SPRIA successfully quantitates both IgG and IgE anti-B.m., they sought to examine the reasons for the SPRIA's apparent superiority in detecting IgE anti-B.m. by extracting specific IgG from sera with high levels of IgE and IgG anti-B.m. antibodies. IgE anti-B.m. was then quantitated in these sera using both the SPRIA and ELISA methods. Results indicate that IgG anti-B.m. does not interfere with detection of specific IgE antibody in the SPRIA but does interfere in the ELISA. While ELISA permits detection of IgE anti-B.m. in the absence of competing IgG anti-B.m., as levels of specific IgG increase, the IgE is no longer detectable. These differences between SPRIA and ELISA can be explained by the SPRIA's antigen excess conditions which assure that there are sufficient antigens both to detect all anti-B.m. antibodies present in the serum and to adequately represent all antigen specificities in the crude B.m. extract. Their findings commend the use of SPRIA methods over ELISA in assessment of B.m.-specific IgE antibody in filariasis and indicate a potential role for SPRIA methods in absolute quantitation of specific serum antibodies. (Auth.)

  1. Quantitation of parasite-specific human IgG and IgE in sera: evaluation of solid-phase RIA and ELISA methodology

    Energy Technology Data Exchange (ETDEWEB)

    Hamilton, R G [Johns Hopkins Univ., Baltimore, MD (USA). Dept. of Medicine; Hussain, R; Ottesen, E A [National Inst. of Allergy and Infectious Diseases, Bethesda, MD (USA); Adkinson, Jr, N F [Johns Hopkins Univ., Baltimore, MD (USA). School of Medicine

    1981-07-17

    The authors have developed a non-competitive solid-phase radioimmunoassay (SPRIA) to quantitate both human IgE and IgG antibodies against soluble adult antigens of Brugia malayi (B.m.), a filarial parasite causing extensive infection throughout the tropics. Previously enzyme-linked immunosorbent assays (ELISA) had been used to detect ..mu..g/ml levels of IgG anti-B.m., but IgE antibodies were difficult to detect in this system. Since the SPRIA successfully quantitates both IgG and IgE anti-B.m., they sought to examine the reasons for the SPRIA's apparent superiority in detecting IgE anti-B.m. by extracting specific IgG from sera with high levels of IgE and IgG anti-B.m. antibodies. IgE anti-B.m. was then quantitated in these sera using both the SPRIA and ELISA methods. Results indicate that IgG anti-B.m. does not interfere with detection of specific IgE antibody in the SPRIA but does interfere in the ELISA. While ELISA permits detection of IgE anti-B.m. in the absence of competing IgG anti-B.m., as levels of specific IgG increase, the IgE is no longer detectable. These differences between SPRIA and ELISA can be explained by the SPRIA's antigen excess conditions which assure that there are sufficient antigens both to detect all anti-B.m. antibodies present in the serum and to adequately represent all antigen specificities in the crude B.m. extract. Their findings commend the use of SPRIA methods over ELISA in assessment of B.m.-specific IgE antibody in filariasis and indicate a potential role for SPRIA methods in absolute quantitation of specific serum antibodies.

  2. MAST CELLS, MAST/STEM CELL GROWTH FACTOR RECEPTOR (C-KIT/CD117 AND IGE MAY BE INTEGRAL TO THE PATHOGENESIS OF ENDEMIC PEMPHIGUS FOLIACEUS

    Directory of Open Access Journals (Sweden)

    Ana Maria Roselino

    2013-11-01

    Full Text Available Introduction: Pemphigus foliaceus (PF is endemic in some South American countries, especially in Colombia and Brazil; in Brazil, it is also known as fogo selvagem (FS. We aimed to study the presence of mast cells and the expression of the mast/stem cell growth factor receptor (c-kit/CD117 in PF skin biopsies, as well as the role of IgE in the disease pathogenesis. Methods: Forty-four skin biopsies from patients affected by endemic PF (EPF (30 patients from El Bagre, Colombia, and 14 from the northeastern region of São Paulo State, Brazil, 48 control biopsies from Colombian and Brazilian endemic areas, and additional control biopsies from none endemic areas in Colombia and the USA non were studied. Immunohistochemistry (IHC was performed to evaluate skin biopsies with anti-mast cell tryptase (MCT, anti-c-kit and anti-IgE antibodies. We also searched for serum IgE in 30 EPF and 30 non-atopic controls from the El Bagre region via ELISA. In our El Bagre patients and controls, we also searched for IgE in skin samples by direct immunofluorescence. Results: In 100% of the EPF biopsies, MCT, c-kit and IgE were identified with stronger expression relative to control biopsies, especially in the inflammatory infiltrates around upper dermal blood vessels and dermal eccrine glands. IgE staining was positive along the BMZ in some EPF skin samples. The DIF results confirmed a linear deposition of IgE at the BMZ. Increased IgE serum levels were also noted in PF patients relative to controls.. Conclusions: In patients with EPF, the observed increased expression of MCT, c-kit and IgE in lesional skin, associated with higher serum IgE levels may indicate possible IgE participation in the antigenic response.

  3. Separation and mapping of multiple genes that control IgE level in Leishmania major infected mice

    Czech Academy of Sciences Publication Activity Database

    Bádalová, J.; Svobodová, M.; Havelková, Helena; Vladimirov, Vladimir; Vojtíšková, Jarmila; Engová, J.; Pilčík, Tomáš; Volf, P.; Demant, P.; Lipoldová, Marie

    2002-01-01

    Roč. 2002, č. 3 (2002), s. 187-195 ISSN 1466-4879 Institutional research plan: CEZ:AV0Z5052915 Keywords : IgE * quantitative trait * Leishmania major, Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.060, year: 2002

  4. Variability of crossreactivity of IgE antibodies to group I and V allergens in eight grass pollen species

    NARCIS (Netherlands)

    van Ree, R.; Driessen, M. N.; van Leeuwen, W. A.; Stapel, S. O.; Aalberse, R. C.

    1992-01-01

    Crossreactivity to Dactylis glomerata, Festuca rubra, Phleum pratense, Anthoxanthum odoratum, Secale cereale, Zea mays, and Phragmites communis of IgE antibodies against Lol p I or Lol p V was investigated by means of RAST-inhibition. Within a group of sera the degree of crossreactivity was

  5. Serum IgE Induced Airway Smooth Muscle Cell Remodeling Is Independent of Allergens and Is Prevented by Omalizumab

    Science.gov (United States)

    Roth, Michael; Zhao, Feng; Zhong, Jun; Lardinois, Didier; Tamm, Michael

    2015-01-01

    Background Airway wall remodeling in allergic asthma is reduced after treatment with humanized anti-IgE-antibodies. We reported earlier that purified IgE, without the presence of allergens, is sufficient to induce airway wall remodeling due to airway smooth muscle cell (ASMC) activity deposing extracellular matrix. Objective We postulate that IgE contained in serum of allergic asthma patients, in the absence of allergens, stimulates ASMC remodeling activities and can be prevented by anti-IgE antibodies. Methods Isolated human ASMC were exposed to serum obtained from: (i) healthy controls, or patients with (ii) allergic asthma, (iii) non-allergic asthma, and (iv) atopic non-asthma patients. Proliferation and the deposition of collagens and fibronectin were determined after 3 and 5 days. Results Serum from patients with allergies significantly stimulated: (i) ASMC proliferation, (ii) deposition of collagen type-I (48 hours) and (iii) of fibronectin (24 hours). One hour pre-incubation with Omalizumab prevented these three effects of allergic serum, but had no significant effect on serum from healthy donors or non-allergic asthma patients. Interestingly, the addition of allergens did not further increase any of the IgE effects. Conclusion and Clinical Relevance Our data provides experimental evidence that the beneficial effect of Omalizumab on airway wall remodeling and improved lung function may be due to its direct action on IgE bound ASMC. PMID:26332463

  6. Serum IgE Induced Airway Smooth Muscle Cell Remodeling Is Independent of Allergens and Is Prevented by Omalizumab.

    Directory of Open Access Journals (Sweden)

    Michael Roth

    Full Text Available Airway wall remodeling in allergic asthma is reduced after treatment with humanized anti-IgE-antibodies. We reported earlier that purified IgE, without the presence of allergens, is sufficient to induce airway wall remodeling due to airway smooth muscle cell (ASMC activity deposing extracellular matrix.We postulate that IgE contained in serum of allergic asthma patients, in the absence of allergens, stimulates ASMC remodeling activities and can be prevented by anti-IgE antibodies.Isolated human ASMC were exposed to serum obtained from: (i healthy controls, or patients with (ii allergic asthma, (iii non-allergic asthma, and (iv atopic non-asthma patients. Proliferation and the deposition of collagens and fibronectin were determined after 3 and 5 days.Serum from patients with allergies significantly stimulated: (i ASMC proliferation, (ii deposition of collagen type-I (48 hours and (iii of fibronectin (24 hours. One hour pre-incubation with Omalizumab prevented these three effects of allergic serum, but had no significant effect on serum from healthy donors or non-allergic asthma patients. Interestingly, the addition of allergens did not further increase any of the IgE effects.Our data provides experimental evidence that the beneficial effect of Omalizumab on airway wall remodeling and improved lung function may be due to its direct action on IgE bound ASMC.

  7. Are basophil histamine release and high affinity IgE receptor expression involved in asymptomatic skin sensitization?

    DEFF Research Database (Denmark)

    Jensen, Bettina Margrethe; Assing, K; Jensen, Lone Hummelshøj

    2006-01-01

    Immunoglobulin (Ig)E-sensitized persons with positive skin prick test, but no allergy symptoms, are classified as being asymptomatic skin sensitized (AS). The allergic type 1 disease is dependant on IgE binding to the high affinity IgE-receptor (FcepsilonRI) expressed on basophils and mast cells....

  8. A sensitive and reproducible method for the determination of subnanogram quantities of immunoglobulin E (IgE)

    DEFF Research Database (Denmark)

    Poulsen, L K; Malling, H J; Søndergaard, I

    1986-01-01

    of a comparison between the Maxisorp assay and paper radioimmunosorbent test (PRIST) and a correlation coefficient of 0.98 (P less than 0.001) was obtained. We conclude that the Maxisorp assay is a fast and reliable assay for IgE determination in cord blood, cell culture supernatants and other highly diluted Ig...

  9. Differential expression of IgE and IgG4 specific antibody responses in asymptomatic and chronic human filariasis

    NARCIS (Netherlands)

    Kurniawan, A.; Yazdanbakhsh, M.; van Ree, R.; Aalberse, R.; Selkirk, M. E.; Partono, F.; Maizels, R. M.

    1993-01-01

    A population of 164 adult individuals resident in an area endemic for Brugia malayi lymphatic filariasis has been studied for humoral immune responses to filarial parasites. Antibody levels to Ag extracted from adult worms were determined for each of the IgG subclasses, for IgM and for IgE. The

  10. Analytical criteria for performance characteristics of IgE binding methods for evaluating safety of biotech food products

    NARCIS (Netherlands)

    Holzhauser, Thomas; van Ree, Ronald; Poulsen, Lars K.; Bannon, Gary A.

    2008-01-01

    There is detailed guidance on how to perform bioinformatic analyses and enzymatic degradation studies for genetically modified crops under consideration for approval by regulatory agencies; however, there is no consensus in the scientific community on the details of how to perform IgE serum studies.

  11. Frequent IgE sensitization to latex, cow's milk, and egg in children with short bowel syndrome.

    Science.gov (United States)

    Mazon, Angel; Solera, Eva; Alentado, Noemi; Oliver, Fernando; Pamies, Rafael; Caballero, Luis; Nieto, Antonio; Dalmau, Jaime

    2008-03-01

    Children with short bowel syndrome (SBS) undergo frequent operations, so they are at risk for sensitizing to latex. There have been isolated reports of sensitization to food in these children. In a cross-sectional study, we assessed sensitization to latex, cow's milk, and egg with skin prick tests (SPT) and serum-specific immunoglobulin E (IgE) in 14 children with SBS. Data were collected about the number of operations with latex devices, serum total IgE, and history of feeding with milk formula. Ten children were sensitized to latex (specific IgE median: 6.7 kU/l, range: 0.5-33). Compared with those non-sensitized, sensitized children had significantly (p range: 0.5-21.1 kU/l), and five to egg (specific IgE median: 0.68, range: 0.58-2.17 kU/l). Except for some isolated days with cow's milk formula, the children had been initially fed with a diet without intact cow's milk proteins. Sensitization to latex, cow's milk, and egg is very frequent in children with SBS. They should be treated in a latex-free environment since the very early stages of the disease, and should be routinely studied regarding food sensitization, as this might contribute as an added factor in the chronic diarrhea of these patients.

  12. The adjuvant effect of phthalate exposure on IgE sensitisation in early childhood

    DEFF Research Database (Denmark)

    Callesen, M.; Weschler, Charles J.; Jensen, T.

    2012-01-01

    on internationally accepted criteria. Result: In the group of randomly selected children IgE sensitization was associated with the total phthalate exposure (P ...E sensitization in children. Method: A cross-sectional case-cohort study (n = 500) based on 2835 children, aged 3–5 years, responding to a questionnaire in the Danish Indoor Environment and Children’s Health study consisted of 300 subjects randomly selected and 200 cases with at least two parentally reported...... doctor diagnosed allergic diseases (asthma, allergic rhinoconjunctivitis or atopic dermatitis). The same physician conducted a clinical examination of all the 500 children including a structured interview on allergic heredity, clinical and medical history Specific serum-IgE against inhalant and food...

  13. IgG and IgE antibodies to Chironomidae in asthmatic patients.

    Science.gov (United States)

    Yamashita, N; Ito, K; Nakagawa, T; Haida, M; Okudaira, H; Nakada, S; Miyamoto, T; Shibuya, T; Kamei, K; Sasa, M

    1987-01-01

    IgG antibodies to Chironomidae and its correlations to radioallergosorbent and skin reactions were examined with the aim of clarifying the relationship between asthma and Chironomidae. The level of specific IgG antibody in asthmatic patients (0.698 +/- 0.034, n = 104) was significantly greater than that in normal subjects (0.367 +/- 0.032, n = 52) (P less than 0.01). The specific IgG level was not correlated to skin reaction, nor to IgE RAST scores. Specific IgG1 and IgG4 levels in asthmatic patients were significantly greater than in control subjects (n = 14) (P less than 0.01). Images Fig. 5 PMID:3652516

  14. Prozone effect of serum IgE levels in a case of plasma cell leukemia

    Directory of Open Access Journals (Sweden)

    Talamo Giampaolo

    2010-09-01

    Full Text Available Abstract We describe a case of multiple myeloma (MM and secondary plasma cell leukemia (PCL secreting IgE-kappa immunoglobulin. To our knowledge, only 2 cases of IgE-producing secondary PCL have been reported in the medical literature. In our patient, the only tumor marker available for monitoring the therapeutic response to chemotherapy and allogeneic stem cell transplantation was the quantitative M component at serum protein electrophoresis (SPEP, because serum free light chains were in the normal range, Bence-Jones proteinuria was absent, and quantitative serum IgE levels provided inaccurate and erratic results, due to the prozone effect. This is a laboratory phenomenon that occurs when antigen excess interferes with antibody-based methods requiring immune complex formation for detection. It is important to recognize the presence of a prozone effect, because it can produce falsely normal results, and therefore it could lead clinicians to incorrect assessment of the response to therapy.

  15. Prozone effect of serum IgE levels in a case of plasma cell leukemia.

    Science.gov (United States)

    Talamo, Giampaolo; Castellani, William; Dolloff, Nathan G

    2010-09-10

    We describe a case of multiple myeloma (MM) and secondary plasma cell leukemia (PCL) secreting IgE-kappa immunoglobulin. To our knowledge, only 2 cases of IgE-producing secondary PCL have been reported in the medical literature. In our patient, the only tumor marker available for monitoring the therapeutic response to chemotherapy and allogeneic stem cell transplantation was the quantitative M component at serum protein electrophoresis (SPEP), because serum free light chains were in the normal range, Bence-Jones proteinuria was absent, and quantitative serum IgE levels provided inaccurate and erratic results, due to the prozone effect. This is a laboratory phenomenon that occurs when antigen excess interferes with antibody-based methods requiring immune complex formation for detection. It is important to recognize the presence of a prozone effect, because it can produce falsely normal results, and therefore it could lead clinicians to incorrect assessment of the response to therapy.

  16. An Allergen Portrait Gallery: Representative Structures and an Overview of IgE Binding Surfaces

    Directory of Open Access Journals (Sweden)

    Ovidiu Ivanciuc

    2010-10-01

    Full Text Available Recent progress in the biochemical classification and structural determination of allergens and allergen–antibody complexes has enhanced our understanding of the molecular determinants of allergenicity. Databases of allergens and their epitopes have facilitated the clustering of allergens according to their sequences and, more recently, their structures. Groups of similar sequences are identified for allergenic proteins from diverse sources, and all allergens are classified into a limited number of protein structural families. A gallery of experimental structures selected from the protein classes with the largest number of allergens demonstrate the structural diversity of the allergen universe. Further comparison of these structures and identification of areas that are different from innocuous proteins within the same protein family can be used to identify features specific to known allergens. Experimental and computational results related to the determination of IgE binding surfaces and methods to define allergen-specific motifs are highlighted.

  17. Nível sérico de IgE total em alergia respiratória: estudo em pacientes com alto risco de infecção por helmintos Total IgE level in respiratory allergy: study of patients at high risk for helminthic infection

    Directory of Open Access Journals (Sweden)

    Décio Medeiros

    2006-08-01

    Full Text Available OBJETIVO: Eosinofilia e elevação de IgE sérica são expressões de atopia, contudo há fatores intervenientes como, por exemplo, as parasitoses intestinais. Esta pesquisa verifica a relação entre IgE sérica total, eosinófilos e IgE específica anti-áscaris em indivíduos portadores de asma e/ou rinite alérgica. MÉTODOS: Estudo do tipo transversal em adolescentes portadores de asma e/ou rinite alérgica que foram examinados quanto ao nível sérico de IgE total, de IgE anti-áscaris e de contagem dos eosinófilos sangüíneos. RESULTADOS: Foram analisados 101 pacientes com idade entre 12 e 21 anos. A mediana da IgE foi 660 UI/mL (P25-75 243,5-1500, e a dos eosinófilos foi 510 células/mm³ (P25-75 284-811. A IgE anti-áscaris foi positiva em 73% (74/101 da amostra, mas houve apenas 33,7% (34/101 de positividade ao parasitológico de fezes. Os coeficientes de correlação encontrados foram: 0,34 (p = 0,001 entre IgE total e eosinófilos, 0,52 (p OBJECTIVE: Eosinophilia and increased serum IgE levels are indicators of atopy; however, other factors can also play a key role, such as intestinal parasitic infections. This study assesses the relationship between total serum IgE, eosinophil count, and anti-Ascaris IgE in individuals with asthma and/or allergic rhinitis. METHODS: A cross-sectional study was carried out in adolescents with asthma and/or allergic rhinitis. The patients had their total serum IgE, anti-Ascaris IgE and eosinophil count measured. RESULTS: A total of 101 patients aged 12 to 21 years were assessed. Median IgE level was 660 IU/mL (P25-75 243.5-1500, and the eosinophil count corresponded to 510 cells/mm³ (P25-75 284-811. Anti-Ascaris IgE was positive in 73% (74/101 of the individuals, but parasitological stool examination yielded positive results in only 33.7% (34/101. The correlation coefficients were the following: 0.34 (p = 0.001 between total IgE level and eosinophil count, 0.52 (p < 0.001 between total IgE level

  18. Pro j 2 is mesquite profilin: molecular characteristics and specific IgE binding activity.

    Science.gov (United States)

    Ali-Sadeghi, Hosein; Khodadadi, Ali; Amini, Akram; Assarehzadegan, Mohammad-Ali; Sepahi, Najmeh; Zarinhadideh, Farnoosh

    2015-06-01

    Pollens from mesquite (Prosopis juliflora) are potent allergen responsible in causing immediate hypersensitivity reactions in susceptible people in tropical countries. This study aimed to clone, express and purify the mesquite pollen profilin (Pro j 2) as well as evaluating its nucleotide sequence homology in order to predict allergenic cross-reactivity with profilins of common allergenic plants. Immunoblotting assay and specific ELISA were applied to determine the immunoreactivity of sera from 35 patients who were allergic to mesquite pollen. The mesquite profilin-coding sequence was cloned into PTZ57R/T vector and amplified. The cDNA of mesquite pollen profilin was then expressed in Escherichia coli using pET-21b (+) vector and puri?ed by one-step Ni2+ a?nity chromatography. IgE binding capacity of the recombinant mesquite profiling (rPro j 2) was analyzed by specific ELISA, immunoblotting, and inhibition assays. cDNA nucleotide sequencing revealed an open reading frame of 399bp encoding for 133 amino acids which belongs to the profilin family. Seventeen patients (17/35, 48.57%) had significant specific IgE level for rPro j 2. Immunodetection and inhibition assays indicated that puri?ed rPro j 2 might be similar as that in the crude extract. Pro j 2, as a new allergen from mesquite pollen, was produced in E. coli with an IgE-reactivity similar to that of its natural counterpart. The amino acid sequences homology analysis of mesquite profilin and several profilin molecules from other plants showed high degree of cross-reactivity among plant-derived profilins from unrelated families.

  19. Vaccination for birch pollen allergy. Induction of affinity-matured or blocking IgG antibodies does not account for the reduced binding of IgE to Bet v 1

    DEFF Research Database (Denmark)

    Svenson, Morten; Jacobi, Henrik H; Bødtger, Uffe

    2003-01-01

    -SAV IgG provided similar inhibition of Bet v 1 binding to IgE at equimolar levels, and cross inhibition studies between IgG and IgE showed low inter-individual differences. Following SAV, all sera reduced Bet v 1 binding to CD23(+) cells, correlating with reduced binding of Bet v 1 to IgE (P

  20. Videojuego con Realidad Virtual

    OpenAIRE

    González Mora, César

    2017-01-01

    El objetivo del proyecto es el desarrollo de un videojuego deportivo que utilice realidad mixta. El videojuego se podrá utilizar con dispositivos de tipo cardboard, y utilizará realidad aumentada para la interacción del jugador con el videojuego. En el desarrollo se utilizará el motor Unity para conseguir una aplicación multiplataforma, y la librería Vuforia para implementar realidad mixta.

  1. Sistemas integrados con Arduino

    OpenAIRE

    EL YAKOUTI, MOHAMMED

    2017-01-01

    Design of a robot prototype remotely controllable from Bluetooth using Arduino. Control and testing of sensors and events interacting with Arduino and Bluetooth. Diseño de un prototipo de robot controlable remotamente con Bluetooth utilizando Arduino. Control y verificación de los sensores y eventos que interactúan mediante el Arduino y el Bluetooth. El Yakouti, M. (2017). Sistemas integrados con Arduino. http://hdl.handle.net/10251/89274. TFGM

  2. Asthma Symptoms and Specific IgE Levels among Toluene Diisocyanate (TDI) Exposed Workers in Tehran, Iran.

    Science.gov (United States)

    Sharifi, Laleh; Karimi, Akram; Shokouhi Shoormasti, Raheleh; Miri, Sara; Heydar Nazhad, Hassan; Bokaie, Saied; Fazlollahi, Mohammad Reza; Sadeghniiat Haghighi, Khosro; Pourpak, Zahra; Moin, Mostafa

    2013-01-01

    Toluene diisocyanate (TDI) is an imperative chemical substance used in the production of polyurethane foams, elastomers, paints and coatings that cause a variety of health problems in workers who are exposed in work places. This study aimed to determine the asthma symptoms and serum specific IgE levels in TDI exposed workers and comparing the results with healthy control group. All the plants that use TDI in the manufacturing of paint and glue in the west of Tehran Province entered to the study and all the workers (550) completed modified initial questionnaire of the NIOSH, the questions were consisted of asthma symptoms. For each symptomatic exposed worker one healthy, sex and age matched control selected. Total IgE and Specific TDI IgE tests were done for each case and control groups. Among 550 TDI exposed workers, 26(4.7%) had asthma symptoms. Nine (34.6%) of symptomatic workers who were exposed to TDI were active cigarette consumer versus 3(11.5%) unexposed workers, P=0.049(CI= 0.953-17.29) OR=4.059. Nine (34.6%) workers had positive family history of atopy versus 1(3.8%) unexposed workers, P=0.0138 (CI= 1.45-305.41) OR=13.24. TDI specific IgE was found in 2 TDI exposed workers and 1 unexposed worker (P=0.5). Mean of total IgE was 339.05 in exposed workers (P=0.201). This study provides clinical and paraclinical data of workers exposed to TDI and points to a relation between atopy and smoking habit with asthma symptoms that offer preventing recommendations for TDI exposed workers and their heath administrators.

  3. Total and allergen-specific IgE levels during and after pregnancy in relation to maternal allergy.

    Science.gov (United States)

    Sandberg, Martina; Frykman, Anne; Jonsson, Yvonne; Persson, Marie; Ernerudh, Jan; Berg, Göran; Matthiesen, Leif; Ekerfelt, Christina; Jenmalm, Maria C

    2009-07-01

    Type 2 T-helper cell (Th2)-skewed immunity is associated with successful pregnancy and the ability to easily direct immune responses to a Th2-polarised profile may be an evolutionary benefit. The Th2-like immunity associated with allergic disease might generate favourable effects for the maintenance of pregnancy, but could also promote development of Th2-like immune responses and allergic disease in the offspring. The aim of this study was to explore, by using IgE as a stable proxy for Th2, the Th1/Th2 balance in allergic and non-allergic women by measuring allergen-specific and total IgE antibody levels in plasma during pregnancy and after delivery. Specific and total IgE antibody levels were determined by ImmunoCAP technology at five occasions during pregnancy (gestational weeks 10-12, 15-16, 25, 35 and 39), as well as at 2 and 12 months after delivery. Thirty-six women without and 20 women with allergic symptoms were included, of whom 13 were sensitised with allergic symptoms and 30 were non-sensitised without allergic symptoms. The levels of total IgE, but not allergen-specific IgE, were increased during early pregnancy when compared to 12 months after delivery in the sensitised women with allergic symptoms, but not in the non-sensitised women without allergic symptoms (ppregnancy only in the sensitised women with allergic symptoms indicates that allergy is associated with an enhanced Th2 deviation during pregnancy.

  4. Exposure to household endotoxin and total and allergen-specific IgE in the US population

    International Nuclear Information System (INIS)

    Min, Kyoung-Bok; Min, Jin-Young

    2015-01-01

    Background: Although endotoxin has strong pro-inflammatory properties, endotoxin-allergy relationship in adults and children have been inconsistent. Objectives: We investigated the association between household endotoxin levels and total immunoglobulin E (IgE) or specific IgE in the US general population, classified into three age ranges: children/adolescent, adults, and older adults. Methods: We analyzed the 2005–2006 National Health and Nutrition Examination Surveys. A total of 5220 participants for whom serum IgE and household endotoxin data were available was included in the analyses. Results: Exposure to endotoxin reduced the risk for allergic sensitization, especially in specific IgE to plants (OR in Quartile 3 = 0.58; 95% CI = 0.44–0.76) and pets (OR in Quartile 3 = 0.62; 95% CI = 0.41–0.92), for children/adolescents. In contrast, the risk among adults and older adults increased with increasing endotoxin levels. Conclusions: Our findings suggest that the effect of endotoxin on allergic reaction is likely to depend on age. - Highlights: • Findings regarding the endotoxin-allergy relationship in adults and children are inconsistent. • We investigated the association of endotoxin with total and specific IgE in US population. • The association between endotoxin levels and allergic markers is likely to depend on age. • Exposure to endotoxin reduced the risk for allergic sensitization for children/adolescents. • The risk among adults and older adults increased with increasing endotoxin levels. - Exposure to endotoxin reduced the risk for allergic sensitization for children/adolescents, but decreased the risk among adults and older

  5. Natural attenuation in contaminated soils with hydrocarbons; Atenuacion natural en suelos contaminados con hidrocarburos

    Energy Technology Data Exchange (ETDEWEB)

    Corona Ramirez, L; Iturbide Arguelles, R [Facultad de Ingenieria, UNAM, Mexico, D.F. (Mexico)

    2005-06-01

    A contaminated soil experiment was performed using simples from a refinery, containing oil derivative hydrocarbons, specifically those with high concentrations of polyaromathic hydrocarbons (PAH). The testing consisted in 7 pans with 7 kg of soil, the preparation of 6 pans under specific conditions and one as a blank, the conditions were: water content (15 y 30%), addition a non-ionic surfactant. The process consisted in the daily aeration and water control of the samples. The PAH were analyzed: anthracene, benzo(a) pyrene, chrysene, phenanthrene and naphthalene. The results after 8 weeks showed a gradual degradation of PAH, indicating a better removal obtained when the water content was 30% with nutrients addition. [Spanish] Se realizo un experimento con suelo contaminado proveniente de una refineria, el cual contaba con hidrocarburos derivados de petroleo, especificamente con concentraciones elevadas de hidrocarburos poliaromaticos (HAP). El estudio consistio en preparar 7 cajones con 7 kg de suelo, cada uno con las siguientes condiciones: S1suelo contaminado con hidrocarburos y 15% de contenido de agua. S2 suelo contaminado con hidrocarburo y adicion de Nitrogeno y Fosforo (N y P) con 15% de contenido de agua. S3 suelo contaminado con hidrocarburo y adicion de N y P mas un surfactante no ionico, Emulgin W600, con 15% de contenido de agua. S4 igual a S1 pero con 30% de contenido de agua. S5 igual a S2, con 30% de contenido de agua. S6 igual S3 con 30% de contenido de agua. S7 suelo contaminado testigo, sin control de humedad y sin aireacion. La experimentacion consistio en airear el suelo diariamente y controlar el contenido de agua de manera que este fuera constante. Los resultados, indican que la mejor remocion se obtuvo para el contenido de agua de 30%, con adicion de nutrientes y surfactante. Los compuestos con mayor eficiencia de remocion para todas las opciones son naftaleno y antraceno. Por lo tanto, de acuerdo con los resultados, los compuestos

  6. Rasgos de personalidad (ZKPQ en pacientes con fibromialgia: un estudio de casos y controles

    Directory of Open Access Journals (Sweden)

    Silvia Albiol

    2014-10-01

    Full Text Available Este es el primer estudio llevado a cabo para determinar la relación entre la fibromialgia y el Zuckerman-Kuhlman Personality Questionnaire (ZKPQ, y uno de los pocos que tratan de establecer la relación entre dicha entidad diagnóstica y la personalidad normal. Una muestra de mujeres adultas diagnosticadas de fibromialgia (n = 47 fue emparejada por edad y sexo con un grupo control (n = 94. La comparación de ambos grupos nos describe un perfil de personalidad caracterizado por elevadas puntuaciones en Neuroticismo y bajas en Sociabilidad. Un análisis multivariante muestra que puntuaciones elevadas en Neuroticismo y bajas en Intolerancia al Aislamiento son factores predictores de alta probabilidad de ser diagnosticado de fibromialgia. El ZKPQ con sus subescalas específicas y una escala de control proporciona una descripción de la personalidad normal más pormenorizada. Se concluye que médicos y psicólogos trabajen conjuntamente en la construcción de un paradigma donde la perspectiva médica y la psicológica sean ambas consideradas para una mejor conceptualización de la fibromialgia.

  7. Investigando con personas con dificultades de aprendizaje

    Directory of Open Access Journals (Sweden)

    Borja González Luna

    2013-12-01

    Full Text Available El artículo muestra los orígenes de lo que Walmsley (2008 denomina «investigación inclusiva». Para comprender qué se entiende por investigación inclusiva tenemos que remontarnos a los debates epistemológicos sobre las metodologías cuantitativas y cualitativas, acontecidos en la década de los 90, en torno a la revista Disability & Society. A partir de una síntesis de dichos debates, focalizados en el ámbito de la «discapacidad intelectual y del desarrollo», se exponen dos estrategias de colaboración con dicha población: a una aproximación etnográfica (de trabajo grupal, y b una aproximación biográfica (de trabajo individual. A continuación se esboza un posible diseño de trabajo de campo que intenta superar el paradigma cualitativo «clásico» con el objetivo de incluir a dicho colectivo más allá del rol de «sujetos de la investigación». Para finalizar se recoge el debate sobre la accesibilidad de los resultados de la investigación a los participantes en dichas investigaciones, y con ello la necesaria innovación en el ámbito de las «devoluciones» de los resultados, cuando se trata de incluir a personas que presentan limitaciones para la comprensión del lenguaje abstracto oral y/o escrito.

  8. Estudio de la conciliación del área personal, familiar y profesional en enfermeras/os hospitalarios con cargas familiares

    OpenAIRE

    Martínez López, Nuria

    2017-01-01

    Las familias y las mujeres en particular, que trabajan fuera del hogar, necesitan conciliar no solo el trabajo y las responsabilidades familiares, también el área personal o tiempo dedicado a sí mismas. La profesión enfermera, mayoritariamente femenina y con una elevada carga física y emocional, resulta el marco idóneo para estudiar cómo se concilia el área personal, familiar y profesional, así como los aspectos que influyen en la conciliación. Hemos estudiado a las enfermeras/os con cargas ...

  9. Calidad de vida relacionada con la salud de adolescentes latinoamericanos

    Directory of Open Access Journals (Sweden)

    Dartagnan Pinto Guedes

    2014-01-01

    Full Text Available OBJETIVO: El objetivo del presente trabajo es determinar diferencias en relación con el sexo, la edad y el país de origen, en los componentes de la calidad de vida relacionada con la salud (CVRS, en una muestra de adolescentes de tres ciudades localizadas en Argentina, Brasil y Chile, utilizando datos recogidos mediante un cuestionario validado y reconocido internacionalmente: el KIDSCREEN-52. MÉTODOS: Se aplicó el cuestionario KIDSCREEN-52 a 1 357 adolescentes con edades comprendidas entre 12 y 17 años (48,6% varones en muestras seleccionadas en los tres países. Se utilizó el análisis de varianza univariada (ANCOVA y se identificaron diferencias entre países de los adolescentes, sexo y edad en cada componente de CVRS. RESULTADOS: Los datos indican diferencias significativas en componentes específicos de CVRS entre los tres países. Los varones consiguieron puntuaciones significativamente más elevadas que las mujeres en los componentes Bienestar físico (P < 0,001, Bienestar psicológico (P = 0,019, Estado de ánimo y emociones (P < 0,001, Autopercepción (P = 0,001, Autonomía (P < 0,001 y Relación con los padres y vida familiar (P = 0,008. Las puntuaciones medias atribuidas a los componentes Bienestar físico (P = 0,001, Bienestar psicológico (P = 0,001, Autopercepción (P = 0,038, Autonomía (P = 0,001, Relación con los padres y vida familiar (P = 0,001, Entorno escolar (P = 0,001 y Recursos económicos (P = 0,022 muestran un descenso significativo con el avance de la edad; en cuanto al componente Rechazo (social/bullying, los puntajes medios encontrados aumentaron significativamente con la edad (P < 0,001. CONCLUSIONES: Las evidencias encontradas sugieren que las intervenciones en prevención y promoción de la salud deberían ser concebidas para llegar a los grupos-objetivo específicos y tendrían que contemplar acciones de acuerdo con el sexo y la edad de los adolescentes.

  10. Valor predictor del cociente saturación hemoglobina/fio2 en pacientes pediátricos con insuficiencia respiratoria aguda debida a neumonía tratados con ventilación no invasiva. Predictive value of the pulse oximetric saturation/fraction of inspired oxygen ratio in pediatric patients with acute respiratory failure due to pneumonia treated with non-invasive ventilation

    OpenAIRE

    Palanca Arias, Daniel

    2014-01-01

    RESUMEN: El uso de la ventilación no invasiva (VNI) en pacientes con fracaso respiratorio hipoxémico debido a neumonía es controvertido, alcanzando elevadas tasas de fracaso en el Síndrome de Distrés Respiratorio Agudo (SDRA). Recientemente, el cociente SpO2/FiO2 (SF) se ha equiparado al cociente paO2/FiO2 (PF) para la valoración del SDRA. Determinamos en una cohorte de pacientes, diagnosticados de neumonía y tratados con VNI, si el cociente SF es una variable con valor predictor de fraca...

  11. Synthesis of sorbitol by Zymomonas mobilis under high osmotic pressure Síntese de sorbitol por Zymomonas mobilis sob elevada pressão osmótica

    Directory of Open Access Journals (Sweden)

    Márcio de Barros

    2006-09-01

    Full Text Available The bacterium Zymomonas mobilis presents potential for sorbitol production when grown in culture medium with high sugar concentration. Sorbitol is produced and accumulated in the periplasma of the bacterium to protect the cells from the harmful effects of high osmotic pressure that results from the action of invertase on sucrose. The conversion of sucrose into glucose and fructose increases the osmolarity of the medium. However, an excessive increase in the osmotic pressure may decrease the sorbitol production. In this work Saccharomyces cerevisiae invertase was added two media containing sucrose 200 and 300 g.L-1. Sorbitol production in sucrose at 200 g.L-1 was 42.35 and 38.42 g.L-1, with and without the invertase treatment, respectively. In the culture medium with 300 g.L-1 sucrose, production reached 60.4 g.L-1 and with invertase treatment was 19.14 g.L-1. These results indicated that the excessive rise in osmotic pressure led to a significant decrease in sorbitol production by the Zymomonas mobilis bacterium in the sucrose medium treated with invertase.A bactéria Zymomonas mobilis, apresenta potencial para produção de sorbitol quando crescida em meio com alta concentração de açúcar. O sorbitol produzido é acumulado no periplasma da bactéria para conter os efeitos prejudiciais da elevada pressão osmótica, que resulta pela ação da enzima invertase, que promove hidrólise da sacarose. A conversão da sacarose em glicose e frutose aumentando a osmolaridade do meio. Entretanto, um aumento excessivo na pressão osmótica pode inibir a produção de sorbitol pela bactéria. Este trabalho empregou invertase de Saccharomyces cerevisiae nos meios de fermentação com sacarose a 200 e 300 g.L-1. A produção de sorbitol no meio com sacarose a 200 g.L-1 foi de 42,35 g.L-1 e 38,42 g.L-1 com e sem tratamento com invertase respectivamente. No meio com 300 g.L-1 sem tratamento, a produção foi de 60,42 e com tratamento 19,14 g.L-1. Estes

  12. Omalizumab therapy in atopic dermatitis: depletion of IgE does not improve the clinical course - a randomized, placebo-controlled and double blind pilot study.

    Science.gov (United States)

    Heil, Peter Maximilian; Maurer, Dieter; Klein, Brigitte; Hultsch, Thomas; Stingl, Georg

    2010-12-01

    Our understanding of the pathogenic role of IgE in atopic dermatitis is incomplete. We asked whether blocking free IgE would alter the course of the disease. We administered either omalizumab, a humanized monoclonal mouse antibody against IgE, or placebo subcutaneously for 16 weeks to 20 atopic dermatitis patients and measured immunological and clinical disease parameters. Omalizumab (I) reduced free serum IgE, (II) lowered surface IgE and FcɛRI expression on different peripheral blood mononuclear cells, (III) reduced the saturation of FcɛRI with IgE, (IV) increased the number of free FcɛRI and (V) lowered the number of IgE+, but not of FcɛRI+ cells in skin. The in vivo relevance of these results is evidenced by the increase in the threshold allergen concentration required to give a type I hypersensitivity reaction in the titrated skin test. While not significantly altering the clinical disease parameters, omalizumab treatment led to an improvement of the atopy patch test results in single patients, i.e. an eczematous reaction upon epicutaneous allergen challenge. The interference with immediate and delayed type skin tests may imply that a therapeutic benefit of omalizumab treatment, if present at all, would be seen in patients with acute rather than chronic forms of the disease. © The Authors • Journal compilation © Blackwell Verlag GmbH, Berlin.

  13. Genome-wide association study for levels of total serum IgE identifies HLA-C in a Japanese population.

    Directory of Open Access Journals (Sweden)

    Yohei Yatagai

    Full Text Available Most of the previously reported loci for total immunoglobulin E (IgE levels are related to Th2 cell-dependent pathways. We undertook a genome-wide association study (GWAS to identify genetic loci responsible for IgE regulation. A total of 479,940 single nucleotide polymorphisms (SNPs were tested for association with total serum IgE levels in 1180 Japanese adults. Fine-mapping with SNP imputation demonstrated 6 candidate regions: the PYHIN1/IFI16, MHC classes I and II, LEMD2, GRAMD1B, and chr13∶60576338 regions. Replication of these candidate loci in each region was assessed in 2 independent Japanese cohorts (n = 1110 and 1364, respectively. SNP rs3130941 in the HLA-C region was consistently associated with total IgE levels in 3 independent populations, and the meta-analysis yielded genome-wide significance (P = 1.07×10(-10. Using our GWAS results, we also assessed the reproducibility of previously reported gene associations with total IgE levels. Nine of 32 candidate genes identified by a literature search were associated with total IgE levels after correction for multiple testing. Our findings demonstrate that SNPs in the HLA-C region are strongly associated with total serum IgE levels in the Japanese population and that some of the previously reported genetic associations are replicated across ethnic groups.

  14. IgE actions on CD4+ T cells, mast cells, and macrophages participate in the pathogenesis of experimental abdominal aortic aneurysms

    DEFF Research Database (Denmark)

    Wang, Jing; Lindholt, Jes S; Sukhova, Galina K

    2014-01-01

    Immunoglobulin E (IgE) activates mast cells (MCs). It remains unknown whether IgE also activates other inflammatory cells, and contributes to the pathogenesis of abdominal aortic aneurysms (AAAs). This study demonstrates that CD4+ T cells express IgE receptor FcεR1, at much higher levels than do CD......8+ T cells. IgE induces CD4+ T-cell production of IL6 and IFN-γ, but reduces their production of IL10. FcεR1 deficiency (Fcer1a-/-) protects apolipoprotein E-deficient (Apoe-/-) mice from angiotensin-II infusion-induced AAAs and reduces plasma IL6 levels. Adoptive transfer of CD4+ T cells (but...... with AAAs had significantly higher plasma IgE levels than those without AAAs. This study establishes an important role of IgE in AAA pathogenesis by activating CD4+ T cells, MCs, and macrophages and supports consideration of neutralizing plasma IgE in the therapeutics of human AAAs....

  15. Study of the role of serum folic acid in atopic dermatitis: A correlation with serum IgE and disease severity

    Directory of Open Access Journals (Sweden)

    Maha A Shaheen

    2011-01-01

    Full Text Available Background : Most atopic dermatitis (AD patients have elevated serum immunoglobulin E (IgE. Impaired folic acid (FA metabolism was found to reduce the intracellular methyl donor pool, associated with a higher prevalence of atopy. Aim : To assess serum IgE and FA in AD patients and to correlate their levels with the disease severity, and with each other. Materials and Methods : Twenty patients with AD were assessed for serum FA and IgE, compared with 20 age- and sex-matched controls. Patients were classified into three groups (mild, moderate, and severe AD based on clinical severity according to Nottingham index. In both patients and controls, serum IgE was measured using Enzyme-linked immunosorbent assay technique and serum FA was measured using Microparticle Enzyme Immunoassay technique. Results : Serum FA levels were lower in AD patients compared with controls, but the difference was not statistically significant. FA levels did not show statistically significant difference among disease severity groups and did not correlate with serum IgE levels. On the other hand, serum IgE levels were significantly elevated in AD patients compared with controls, and among AD patients, its levels were significantly elevated in severe AD compared with mild and moderate disease. Conclusion : Serum IgE is useful in assessment of AD severity and activity. FA contribution to AD needs further investigations.

  16. Lol p I-specific IgE and IgG synthesis by peripheral blood mononuclear cells from atopic subjects in SCID mice.

    Science.gov (United States)

    Gagnon, R; Boutin, Y; Hébert, J

    1995-06-01

    The development of an animal model representative of the in vivo situation of human atopic diseases is always of interest for a better understanding of IgE production and regulation. Along these lines, mice with severe combined immunodeficiency (SCID mice) engrafted with lymphocytes from atopic subjects might be a suitable model for such studies. This study aims to analyze the production of Lol p I-specific IgE and IgG antibodies in SCID mice after transplantation of human peripheral blood mononuclear cells from atopic patients sensitive to grass pollens and from nonatopic donors. Peripheral blood mononuclear cells were transplanted into SCID mice, which were then challenged with Lol p I, and antibody responses (IgG and IgE) were analyzed over a 6-week period. Total IgG antibody was measured in each mouse serum after transplantation. Also, most mice (regardless of whether donors were atopic) that were challenged with Lol p I produced specific IgG antibody. Total IgE antibody production was observed only in mice grafted with cells from atopic patients. Lol p I-specific IgE antibodies were also produced after immunization with Lol p I. Although IgG antibody/response tended to plateau, the IgE antibody response increased until it peaked and declined thereafter. Interferon-gamma was detected in sera from mice producing IgE antibody, which supports a possible role of interferon-gamma in the decrease of IgE response. This study suggests that the SCID mouse model could represent an interesting approach to studying specific, total IgG and IgE antibody production, and ultimately their regulation.

  17. Total and specific serum IgE decreases with age in patients with allergic rhinitis, asthma and insect allergy but not in patients with atopic dermatitis

    Directory of Open Access Journals (Sweden)

    Neuber Karsten

    2005-05-01

    Full Text Available Abstract Concerning allergic diseases, the incidence of allergic symptoms, as well as their severity, seems to decrease with age. The decline of onset of allergic symptoms observed in ageing might result from a decrease of serum total and specific IgE. Atopic disorders are complex diseases that involve interactions among several physiological systems, e.g. skin, lung, mucosae, and the immune system. It was the aim of this study to compare the effects of age on total and specific IgE in patients with atopic dermatitis (AD, allergic rhinitis or asthma, and insect allergy, respectively. The study population consisted of 559 individuals (male: 229 and female: 330. Total and allergen specific IgE was measured in every individual. From the whole study population, 113 patients suffered from atopic dermatitis (AD, 132 had allergic rhinitis or asthma, and 314 were tested because of insect allergy. Total and specific serum IgE was significantly decreased as a function of age in patients with allergic rhinitis and asthma and with insect allergy. In contrast, no significant decrease of total and specific serum IgE in old individuals with AD was observed. Additionally, in the group of patients with a total IgE 300 kU/l showed no correlation with age. Immunosenescence does not affect increased IgE levels in atopic patients with AD and/or high serum IgE levels indicating that in these subgroups of patients the atopic propensity remains into advanced age. One may hypothesize that either onset of allergic sensitization during life or the kind of atopic disease influences the correlation between age and IgE synthesis.

  18. [Analysis of clinical features and allergic status of asthmatic patients with positive serum mycosis-specific IgE].

    Science.gov (United States)

    Mou, Yan; Ye, Ling; Gong, Ying; Zhang, Zhi-feng; Jin, Mei-ling

    2013-08-01

    To improve understanding of the clinical characteristics and diagnosis of allergic bronchopulmonary mycosis (ABPM). We retrospectively analyzed the clinical data, including clinical symptoms, laboratory tests, pulmonary function tests and chest CT imaging of 95 asthmatic patients with positive serum mycosis-specific IgE from January 2010 to September 2012 in Zhongshan Hospital Affiliated to Fudan University. Of the 95 patients, 59 cases met the diagnostic criteria of ABPM. There were 34 males and 25 females, with a mean age of (53 ± 4) years and a duration of asthma for (21 ± 4) years. Thirty-six cases showed mycosis hypersensitivity (MH). There were 10 males and 26 females, with a mean age of (46 ± 6) years and a duration of asthma for (16 ± 5) years. Clinical symptoms such as wheeze (52 vs 21, χ(2) = 11.159, P = 0.001), cough (54 vs 27, χ(2) = 4.859, P = 0.030) and expectoration (43 vs 9, χ(2) = 25.731, P = 0.000) were more common in the ABPM group compared to the MH group.In the ABPM group, 58 were A. fumigatus-specific IgE antibody positive, 34 Penicillium-specific IgE antibody positive and 1 only Penicillium-specific IgE antibody positive.While in the MH group, 15 were A.fumigatus-specific IgE antibody positive, 24 Penicillium-specific IgE antibody positive and 17 only Penicillium-specific IgE antibody positive.In the ABPM group, the percentage of positive fumigatus-specific IgE antibody was higher (58 vs 15, χ(2) = 24.500, P = 0.000), while the percentages of dermatophagoides pteronyssinus(21 vs 20, χ(2) = 3.632, P = 0.045) and Dermatophagoides farinae(17 vs 21, χ(2) = 8.118, P = 0.004) were lower. Total serum IgE [(4395 ± 1437) IU/ml vs (276 ± 133) IU/ml, T = 4.384, P = 0.000], peripheral eosinophil percentage[(12.56 ± 1.20)% vs (1.30 ± 0.15)%, t = 8.175, P = 0.000] and count [(2.09 ± 0.43)×10(9)/L vs (0.19 ± 0.04)×10(9)/L, t = 7.032, P = 0.000] were higher in the ABPM group as compared to the MH group.FEV1% slightly declined in the ABPM

  19. Mayor frecuencia de giardiosis en niños con raquitismo comparada con niños sanos de la población de Suárez, Cauca, Colombia

    OpenAIRE

    Oscar L. Sierra; Lidia N. Peñalosa; Yolanda Alarcón

    1998-01-01

    Se examinaron huevos, quistes y trofozoítos de parásitos intestinales en heces de 332 niños sanos y 70 niños con raquitismo descrito como un trastorno heredado debido a malabsorción de calcio por resistencia a la vitamina D en Suárez, departamento del Cauca. La frecuencia total de parasitosis fue de 65,9% y la de poliparasitismo de 10,9%. Se encontraron frecuencias elevadas de giardiosis y poliparasitismo en niños con raquitismo (17,l y 18,6%) comparado con niños sanos (9,O y 9,3%) y dichas d...

  20. Environmental determinants of total IgE among school children living in the rural Tropics: importance of geohelminth infections and effect of anthelmintic treatment

    Directory of Open Access Journals (Sweden)

    Benitez Susana M

    2008-06-01

    Full Text Available Abstract Background The environmental factors that determine the elevated levels of polyclonal IgE observed in populations living in the Tropics are poorly understood but may include geohelminth infections. We investigated the association between geohelminth infections and total IgE levels in school children in rural tropical Ecuador, and assessed the effect on IgE of repeated anthelmintic treatments over a period of 12 months. The study was nested within a cluster-randomized study that randomized 68 schools to receive either 400 mg of albendazole every 2 months over a year or no treatment. We studied random samples of children completing follow-up and representing four groups stratified by the presence of geohelminth infection at baseline and treatment allocation. We measured levels of total IgE and anti-A. lumbricoides IgG (used as a measure of past and current geohelminth infectious exposure in blood samples collected at the start of the study and after 12 months. Results We observed elevated levels of total IgE (compared to standard reference values at the start of the study in this population of school children (geometric mean, 1,004 IU/mL, range 12 to 22,608 IU/mL and baseline IgE levels were strongly associated with parameters of geohelminth infection but not with age, nutritional and socioeconomic status. After 12 months, levels of IgE fell significantly in the treatment (by 35.1% and no treatment (by 10.4% groups, respectively, but the fall was significantly greater in the treatment group. Falls in IgE were independently associated with albendazole treatment, having a baseline geohelminth infection and with high baseline levels of anti-A. lumbricoides IgG. Increases in IgE at 12 months were associated with the presence of geohelminth infections and increasing levels of anti-A. lumbricoides IgG at 12 months independent of treatment allocation. Conclusion The data provide evidence that geohelminth infections are an important determinant

  1. Giochiamo con i robot

    Directory of Open Access Journals (Sweden)

    Andrea Bonarini

    2009-01-01

    Full Text Available "Giochiamo con i robot" e' un laboratorio interattivo per grandi e piccini realizzato per l'edizione 2007 del Festival della Scienza di Genova. Lungo un percorso che va dalla telerobotica alla robotica evolutiva, il laboratorio sviluppa il tema di dare intelligenza ai robot. Questo percorso, le cui tappe sono le varie installazioni, si conclude nella "bottega" dove e' possibile costruire e programmare i propri robot o smontare e modificare quelli esposti durante il percorso didattico. I visitatori sono coinvolti in attivita' ludiche grazie alle quali possonoentrare in contatto con alcune delle idee potenti della robotica,

  2. disegnare con ... Alberto Pratelli

    Directory of Open Access Journals (Sweden)

    Roberto Mingucci

    2017-12-01

    Full Text Available Con questa breve intervista ad Alberto Pratelli, (non a caso scelto per aprire questa nuova rubrica intendia-mo inaugurare un dialogo con personalità significati-ve del Disegno di Architettura, che consenta riflessioni dedicate alle sue varie dimensioni, oggi più che mai da approfondire. La suggestione a farlo, viene da un’idea di Pablo Rodri-guez Navarro ed abbiamo quindi pensato di avviarla proprio in questo numero, che Pablo ha accettato di curare su un tema a lui particolarmente caro.

  3. Taponamiento de goteros y del filtro de discos con agua residual sanitaria de una laguna de maduración.

    OpenAIRE

    Oliveira Batista, Rafael; Alves Soares, Antonio; Zapata Marin, Olga Lucía; Rodrigues de Souza, José Antônio; Vinícius Leite, Caio; Astoni Moreira, Débora

    2013-01-01

    El método de riego localizado, ha sido usado para la aplicación de aguas residuales, debido a la elevada eficiencia en la aplicación del fluido y el bajo riesgo de contaminación del producto agrícola y de los operadores de campo. Sin embargo, los sistemas de riego localizado, poseen emisores que presentan alta susceptibilidad al taponamiento. En el presente trabajo, se analizó el potencial de taponamiento de distintos goteros y del filtro de discos con agua residual sanitaria proveniente de u...

  4. PRoFIT: Modelo forense-IoT con integración de requisitos de privacidad

    OpenAIRE

    Nieto, Ana; Rios, Ruben; Lopez, Javier

    2018-01-01

    [ES] La Internet de las cosas (IoT) complica sobremanera la extracción de evidencias electrónicas que pueden servir de base para una investigación forense. En entornos altamente cambiantes y con una densidad de dispositivos tan elevada, es muy difícil entender completamente el contexto de la ofensa. Es por ello que la cooperación de los individuos, aún no estando directamente implicados en la ofensa, puede ser muy relevante para el analista forense. En este artículo se...

  5. Nuevos esquemas de inmunoterapia específicas con alérgenos

    Directory of Open Access Journals (Sweden)

    José Fernando Cantillo

    2010-09-01

    Con estos nuevos esquemas se han diseñado vacunas basadas en alérgenos recombinantes y variantes o péptidos derivados de éstos, para ser administrados solos o con adyuvantes en preparaciones que favorecen la captación y presentación antigénica por las células dendríticas o tienen como blanco las células efectoras, como mastocitos y basófilos. Los estudios in vitro, en modelos animales y algunos en fase clínica en humanos, indican que estas preparaciones pueden brindar protección frente a la exposición alergénica o mejorar la sintomatología, al inducir la producción de anticuerpos bloqueadores de la actividad de la IgE, de células T reguladoras y de citocinas del perfil Th1.

  6. Can family history and cord blood IgE predict sensitization and allergic diseases up to adulthood?

    DEFF Research Database (Denmark)

    Borrits Pagh Nissen, Susanne; Fomsgaard Kjær, Henrik; Høst, Arne

    2015-01-01

    with high NPV and specificity, but low PPV and sensitivity. CONCLUSION: Although family history and elevated CB-IgE were significantly associated with primarily atopic disease until 26 yr, none of these were strong predictors for subsequent sensitization and allergic symptoms from childhood until early......BACKGROUND: Long-term studies of the predictive value of family history and cord blood IgE level until adulthood are few, and their conclusions have been contradictory. METHODS: Screening of total IgE in 1617 cord blood samples was performed in a Danish birth cohort. All infants with cord blood Ig...... used. RESULTS: A total of 455 infants were included, 188 with CB-IgE ≥0.5 kU/l and 267 with CB-IgE history and elevated CB-IgE were significantly associated to allergic disease until 26 yr. Concerning any allergic...

  7. Conversando con Oriol Bohigas

    OpenAIRE

    Redondo Domínguez, Ernesto; Moya Sala, Joaquim

    2015-01-01

    [EN] Interview with Oriol Bohigas [ES] Entrevista con Oriol Bohigas Redondo Domínguez, E.; Moya Sala, J. (2015). Conversando con… Oriol Bohigas. EGA. Revista de Expresión Gráfica Arquitectónica. 20(26):22-35. doi:10.4995/ega.2015.4061 22 35 20 26

  8. DR Con o:

    African Journals Online (AJOL)

    which could fall under the Ugandan influence. The con-. flict in the ..... The Congolese people and international community within SADC, the AU ..... ments and make peace among themselves. However, one ... friends overnight.There is a great ...

  9. O formoterol e uma dose média/ elevada de corticosteróides inalados são mais eficazes do que uma dose elevada de corticosteróides na asma moderada e grave

    Directory of Open Access Journals (Sweden)

    G. Mitchell

    2003-05-01

    Full Text Available RESUMO: As normas internacionais para o tratamento da asma brônquica recomendam que os doentes com sintomatologia mal controlada com doses baixas/ /intermédias de corticosteróides (CT inalados sejam submetidos a doses mais elevadas de CT e, se necessário, deverá ser adicionado um β2 agonista de longa duração. No entanto, estudos mais recentes demonstraram que a adição deste fármaco a doses baixas/moderadas de CT inalados permite um melhor controlo da sintomatologia do que a duplicação da dose de CT. O formoterol constitui o β2 agonista de eleição devido ao seu início de acção rápida.O objectivo do presente trabalho foi comparar a eficácia da associação do β2 agonista formoterol a doses médias/elevadas de CT inalados com a duplicação da dose do CT em indivíduos com asma moderada/grave mal controlada.Foram avaliados doentes com idade superior a 18 anos com asma moderada e grave que apresentavam um FEV1≥50% do previsto e um aumento≥15% do referido parâmetro após inalação de um broncodilatador de curta duração, tendo sido submetido a terapêutica com CT inalados diariamente (dipropionato de beclometasona 1000 μ - budesonida 800 μg no mês que precedeu o início do estudo em análise. A existência de, pelo menos, dois dos parâmetros seguintes, nos últimos 7 dias do período de run-in, foi imprescindível: sintomatologia interferindo com as actividades de vida diária; interrupção do sono por sintomas nocturnos, necessidade de terapêutica de alívio numa dose≥4 puffs salbutamol/dia; variabilidade diária do PEF (Peak Expiratory Flow≥15%.Foram critérios de exclusão: doentes cuja dose do CT inalado diária foi alterada no último mês; indivíduos submetidos a CT oral ou β2 agonista de longa duração no mês precedente; doentes com dificuldade de utilizar o

  10. O formoterol e uma dose média/ elevada de corticosteróides inalados são mais eficazes do que uma dose elevada de corticosteróides na asma moderada e grave

    Directory of Open Access Journals (Sweden)

    G. Mitchell

    2004-09-01

    Full Text Available RESUMO: As normas internacionais para o tratamento da asma brônquica recomendam que os doentes com sintomatologia mal controlada com doses baixas/ /intermédias de corticosteróides (CT inalados sejam submetidos a doses mais elevadas de CT e, se necessário, deverá ser adicionado um β2 agonista de longa duração. No entanto, estudos mais recentes demonstraram que a adição deste fármaco a doses baixas/moderadas de CT inalados permite um melhor controlo da sintomatologia do que a duplicação da dose de CT. O formoterol constitui o β2 agonista de eleição devido ao seu início de acção rápida.O objectivo do presente trabalho foi comparar a eficácia da associação do β2 agonista formoterol a doses médias/elevadas de CT inalados com a duplicação da dose do CT em indivíduos com asma moderada/grave mal controlada.Foram avaliados doentes com idade superior a 18 anos com asma moderada e grave que apresentavam um FEV1≥50% do previsto e um aumento≥15% do referido parâmetro após inalação de um broncodilatador de curta duração, tendo sido submetido a terapêutica com CT inalados diariamente (dipropionato de beclometasona 1000 μ - budesonida 800 μg no mês que precedeu o início do estudo em análise. A existência de, pelo menos, dois dos parâmetros seguintes, nos últimos 7 dias do período de run-in, foi imprescindível: sintomatologia interferindo com as actividades de vida diária; interrupção do sono por sintomas nocturnos, necessidade de terapêutica de alívio numa dose≥4 puffs salbutamol/dia; variabilidade diária do PEF (Peak Expiratory Flow≥15%.Foram critérios de exclusão: doentes cuja dose do CT inalado diária foi alterada no último mês; indivíduos submetidos a CT oral ou β2 agonista de longa duração no mês precedente; doentes com dificuldade de utilizar o

  11. Identification of Aspergillus (A. flavus and A. niger) Allergens and Heterogeneity of Allergic Patients' IgE Response.

    Science.gov (United States)

    Vermani, Maansi; Vijayan, Vannan Kandi; Agarwal, Mahendra Kumar

    2015-08-01

    Aspergillus species (A. flavus and A. niger) are important sources of inhalant allergens. Current diagnostic modalities employ crude Aspergillus extracts which only indicate the source to which the patient has been sensitized, without identifying the number and type of allergens in crude extracts. We report a study on the identification of major and minor allergens of the two common airborne Aspergillus species and heterogeneity of patients' IgE response to them. Skin prick tests were performed on 300 patients of bronchial asthma and/or allergic rhinitis and 20 healthy volunteers. Allergen specific IgE in patients' sera was estimated by enzyme allergosorbent test (EAST). Immunoblots were performed to identify major/minor allergens of Aspergillus extracts and to study heterogeneity of patients'IgE response to them. Positive cutaneous responses were observed in 17% and 14.7% of patients with A. flavus and A. niger extracts, respectively. Corresponding EAST positivity was 69.2% and 68.7%. In immunoblots, 5 allergenic proteins were identified in A. niger extract, major allergens being 49, 55.4 and 81.5 kDa. Twelve proteins bound patients' IgE in A. flavus extract, three being major allergens (13.3, 34 and 37 kDa). The position and slopes of EAST binding and inhibition curves obtained with individual sera varied from patient to patient. The number and molecular weight of IgE-binding proteins in both the Aspergillus extracts varied among patients. These results gave evidence of heterogeneity of patients' IgE response to major/minor Aspergillus allergens. This approach will be helpful to identify disease eliciting molecules in the individual patients (component resolved diagnosis) and may improve allergen-specific immunotherapy.

  12. IgE antibodies, FcεRIα, and IgE-mediated local anaphylaxis can limit snake venom toxicity.

    Science.gov (United States)

    Starkl, Philipp; Marichal, Thomas; Gaudenzio, Nicolas; Reber, Laurent Lionel; Sibilano, Riccardo; Tsai, Mindy; Galli, Stephen Joseph

    2016-01-01

    Type 2 cytokine-related immune responses associated with development of antigen-specific IgE antibodies can contribute to pathology in patients with allergic diseases and to fatal anaphylaxis. However, recent findings in mice indicate that IgE also can enhance defense against honeybee venom. We tested whether IgE antibodies, IgE-dependent effector mechanisms, and a local anaphylactic reaction to an unrelated antigen can enhance defense against Russell viper venom (RVV) and determined whether such responses can be influenced by immunization protocol or mouse strain. We compared the resistance of RVV-immunized wild-type, IgE-deficient, and Fcer1a-deficient mice after injection of a potentially lethal dose of RVV. A single prior exposure to RVV enhanced the ability of wild-type mice, but not mice lacking IgE or functional FcεRI, to survive challenge with a potentially lethal amount of RVV. Moreover, IgE-dependent local passive cutaneous anaphylaxis in response to challenge with an antigen not naturally present in RVV significantly enhanced resistance to the venom. Finally, we observed different effects on resistance to RVV or honeybee venom in BALB/c versus C57BL/6 mice that had received a second exposure to that venom before challenge with a high dose of that venom. These observations illustrate the potential benefit of IgE-dependent effector mechanisms in acquired host defense against venoms. The extent to which type 2 immune responses against venoms can decrease pathology associated with envenomation seems to be influenced by the type of venom, the frequency of venom exposure, and the genetic background of the host. Copyright © 2015 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  13. IL-4-producing murine T helper cell line provides help for in vitro production of IgE

    DEFF Research Database (Denmark)

    Poulsen, L K; Katamura, K; Ishizaka, K

    1991-01-01

    with nonadherent splenocytes from normal MHC-matched mice. Help provided by the antigen-stimulated T cell line induced significant IgE production (20 ng/ml), along with IgG1 (5 micrograms/ml) and IgM (250 micrograms/ml). Immunoglobulin synthesis in cultures was detectable at day 3-4 and culminated at day 7-8. IL-4...

  14. Staphylococcal superantigen-specific IgE antibodies: degree of sensitization and association with severity of asthma

    OpenAIRE

    Elabras Filho, José; Mello, Fernanda Carvalho de Queiroz; Lupi, Omar; Bica, Blanca Elena Rios Gomes; Papi, José Angelo de Souza; França, Alfeu Tavares

    2016-01-01

    ABSTRACT Objective: To determine the presence of staphylococcal superantigen-specific IgE antibodies and degree of IgE-mediated sensitization, as well as whether or not those are associated with the severity of asthma in adult patients. Methods: This was a cross-sectional study involving outpatients with asthma under treatment at a tertiary care university hospital in the city of Rio de Janeiro, Brazil. Consecutive patients were divided into two groups according to the severity of asthma ba...

  15. Population PK and IgE pharmacodynamic analysis of a fully human monoclonal antibody against IL4 receptor.

    Science.gov (United States)

    Kakkar, Tarundeep; Sung, Cynthia; Gibiansky, Leonid; Vu, Thuy; Narayanan, Adimoolam; Lin, Shao-Lee; Vincent, Michael; Banfield, Christopher; Colbert, Alex; Hoofring, Sarah; Starcevic, Marta; Ma, Peiming

    2011-10-01

    For AMG 317, a fully human monoclonal antibody to interleukin receptor IL-4Rα, we developed a population pharmacokinetic (PK) model by fitting data from four early phase clinical trials of intravenous and subcutaneous (SC) routes simultaneously, investigated important PK covariates, and explored the relationship between exposure and IgE response. Data for 294 subjects and 2183 AMG 317 plasma concentrations from three Phase 1 and 1 Phase 2 studies were analyzed by nonlinear mixed effects modeling using first-order conditional estimation with interaction. The relationship of IgE response with post hoc estimates of exposure generated from the final PK model was explored based on data from asthmatic patients. The best structural model was a two-compartment quasi-steady-state target-mediated drug disposition model with linear and non-linear clearances. For a typical 80-kg, 40-year subject, linear clearance was 35.0 mL/hr, central and peripheral volumes of distribution were 1.78 and 5.03 L, respectively, and SC bioavailability was 24.3%. Body weight was an important covariate on linear clearance and central volume; age influenced absorption rate. A significant treatment effect was observable between the cumulative AUC and IgE response measured. The population PK model adequately described AMG 317 PK from IV and SC routes over a 60-fold range of doses with two dosing strengths across multiple studies covering healthy volunteers and patients with mild to severe asthma. IgE response across a range of doses and over the sampling time points was found to be related to cumulative AMG 317 exposure.

  16. Circulating CXCR5+CD4+ T cells participate in the IgE accumulation in allergic asthma.

    Science.gov (United States)

    Gong, Fang; Zhu, Hua-Yan; Zhu, Jie; Dong, Qiao-Jing; Huang, Xuan; Jiang, Dong-Jin

    2018-05-01

    The pathogenesis of allergic asthma is primarily characterized by abnormality in immunoglobin(Ig)E pathway, suggesting a possible role for follicular helper T cells (Tfh) in the genesis of excessive IgE accumulation. The blood chemokine (C-X-C motif) receptor 5 (CXCR)5 + CD4 + T cells, known as "circulating" Tfh, share common functional characteristics with Tfh cells from germinal centers. The aim of this study was to determine the phenotypes and functions of circulating CXCR5 + CD4 + T cells in allergic asthmatics. Here we found the frequency of the circulating CXCR5 + CD4 + T cells was raised in allergic asthma compared with healthy control (HC). Phenotypic assays showed that activated circulating CXCR5 + CD4 + T cells display the key features of Tfh cells, including invariably coexpressed programmed cell death (PD)-1 and inducible costimulator (ICOS). The frequency of interleukin IL-4 + -, IL-21 + -producing CXCR5 + CD4 + T cells was increased in allergic asthma patients compared with HC. Furthermore, sorted circulating CXCR5 + CD4 + T cells from allergic asthma patients boosted IgE production in coculture assay which could be inhibited by IL-4 or IL-21 blockage. Interestingly, IL-4 + -, IL-21 + -CXCR5 + CD4 + T cells positively correlated with total IgE in the blood. Our data indicated that circulating CXCR5 + CD4 + T cells may have a significant role in facilitating IgE production in allergic asthma patients. Copyright © 2018 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.

  17. Molecular Typing of Staphylococcus aureus Isolated from Patients with Autosomal Dominant Hyper IgE Syndrome

    Directory of Open Access Journals (Sweden)

    Inka Sastalla

    2017-06-01

    Full Text Available Autosomal dominant hyper IgE syndrome (AD-HIES is a primary immunodeficiency caused by a loss-of-function mutation in the Signal Transducer and Activator of Transcription 3 (STAT3. This immune disorder is clinically characterized by increased susceptibility to cutaneous and sinopulmonary infections, in particular with Candida and Staphylococcus aureus. It has recently been recognized that the skin microbiome of patients with AD-HIES is altered with an overrepresentation of certain Gram-negative bacteria and Gram-positive staphylococci. However, these alterations have not been characterized at the species- and strain-level. Since S. aureus infections are influenced by strain-specific expression of virulence factors, information on colonizing strain characteristics may provide insights into host-pathogen interactions and help guide management strategies for treatment and prophylaxis. The aim of this study was to determine whether the immunodeficiency of AD-HIES selects for unique strains of colonizing S. aureus. Using multi-locus sequence typing (MLST, protein A (spa typing, and PCR-based detection of toxin genes, we performed a detailed analysis of the S. aureus isolates (n = 13 found on the skin of twenty-one patients with AD-HIES. We found a low diversity of sequence types, and an abundance of strains that expressed methicillin resistance, Panton-Valentine leukocidin (PVL, and staphylococcal enterotoxins K and Q (SEK, SEQ. Our results indicate that patients with AD-HIES may often carry antibiotic-resistant strains that harbor key virulence factors.

  18. Positive Skin Test or Specific IgE to Penicillin Does Not Reliably Predict Penicillin Allergy

    DEFF Research Database (Denmark)

    Tannert, Line Kring; Mørtz, Charlotte G; Skov, Per Stahl

    2017-01-01

    INTRODUCTION: According to guidelines, patients are diagnosed with penicillin allergy if skin test (ST) result or specific IgE (s-IgE) to penicillin is positive. However, the true sensitivity and specificity of these tests are presently not known. OBJECTIVE: To investigate the clinical relevance...... of a positive ST result and positive s-IgE and to study the reproducibility of ST and s-IgE. METHODS: A sample of convenience of 25 patients with positive penicillin ST results, antipenicillin s-IgE results, or both was challenged with their culprit penicillin. Further 19 patients were not challenged......-IgE measured (T0), and then skin tested and had s-IgE measured 4 weeks later (T1). RESULTS: Only 9 (36%) of 25 were challenge positive. There was an increased probability of being penicillin allergic if both ST result and s-IgE were positive at T0. Positive ST result or positive s-IgE alone did not predict...

  19. Specific IgE response to different grass pollen allergen components in children undergoing sublingual immunotherapy

    Directory of Open Access Journals (Sweden)

    Marcucci Francesco

    2012-06-01

    Full Text Available Abstract Background Grass pollen is a major cause of respiratory allergy worldwide and contain a number of allergens, some of theme (Phl p 1, Phl p 2, Phl p 5, and Phl 6 from Phleum pratense, and their homologous in other grasses are known as major allergens. The administration of grass pollen extracts by immunotherapy generally induces an initial rise in specific immunoglobulin E (sIgE production followed by a progressive decline during the treatment. Some studies reported that immunotherapy is able to induce a de novo sensitisation to allergen component previously unrecognized. Methods We investigated in 30 children (19 males and 11 females, mean age 11.3 years, 19 treated with sublingual immunotherapy (SLIT by a 5-grass extract and 11 untreated, the sIgE and sIgG4 response to the different allergen components. Results Significant increases (p  Conclusions These findings confirm that the initial phase of SLIT with a grass pollen extract enhances the sIgE synthesis and show that the sIgE response concerns the same allergen components which induce IgE reactivity during natural exposure.

  20. fertilizada con diferentes abonos

    Directory of Open Access Journals (Sweden)

    Jorge Alberto Elizondo-Salazar

    2007-01-01

    Full Text Available Producción y calidad de la biomasa de morera (Morus alba fertilizada con diferentes abonos. Se llevó a cabo un experimento en la Estación Experimental “Alfredo Volio Mata” de la Universidad de Costa Rica con el fi n de evaluar la aplicación de 150 kg de N/ha/año proveniente de dos abonos orgánicos: lombriabono y compostaje; y de un fertilizante químico, sobre la producción y calidad de la biomasa de morera. El periodo experimental comprendió un ciclo de 12 meses, iniciando en julio del 2003 y fi nalizando en julio del 2004. Se utilizó una plantación de morera de 12 años de establecida con una densidad de siembra de 27.777 plantas/ ha. Se empleó un diseño de bloques completos al azar con cuatro tratamientos: dos abonos orgánicos, nitrato de amonio (33,5% N y un control. Las plantas se podaron a 0,6 m sobre el nivel del suelo al inicio del ensayo. Durante el periodo experimental, las plantas fueron podadas consecutivamente cada 90 días. Las hojas y los tallos fueron separados y analizados para determinar el contenido de materia seca y proteína cruda. La producción de materia seca fue 23% superior y el contenido de proteína cruda fue signifi cativamente mayor con el nitrógeno químico, mientras que el contenido de materia seca fue menor. No se encontraron diferencias signifi cativas entre el tratamiento control y los tratamientos orgánicos.

  1. Chimeras of Bet v 1 and Api g 1 reveal heterogeneous IgE responses in patients with birch pollen allergy.

    Science.gov (United States)

    Gepp, Barbara; Lengger, Nina; Bublin, Merima; Hemmer, Wolfgang; Breiteneder, Heimo; Radauer, Christian

    2014-07-01

    Characterization of IgE-binding epitopes of allergens and determination of their patient-specific relevance is crucial for the diagnosis and treatment of allergy. We sought to assess the contribution of specific surface areas of the major birch pollen allergen Bet v 1.0101 to binding IgE of individual patients. Four distinct areas of Bet v 1 representing in total 81% of its surface were grafted onto the scaffold of its homolog, Api g 1.0101, to yield the chimeras Api-Bet-1 to Api-Bet-4. The chimeras were expressed in Escherichia coli and purified. IgE binding of 64 sera from Bet v 1-sensitized subjects with birch pollen allergy was determined by using direct ELISA. Specificity was assessed by means of inhibition ELISA. rApi g 1.0101, Api-Bet-1, Api-Bet-2, Api-Bet-3, and Api-Bet-4 bound IgE from 44%, 89%, 80%, 78%, and 48% of the patients, respectively. By comparing the amount of IgE binding to the chimeras and to rApi g 1.0101, 81%, 70%, 75%, and 45% of the patients showed significantly enhanced IgE binding to Api-Bet-1, Api-Bet-2, Api-Bet-3, and Api-Bet-4, respectively. The minority (8%) of the sera revealed enhanced IgE binding exclusively to a single chimera, whereas 31% showed increased IgE binding to all 4 chimeras compared with rApi g 1.0101. The chimeras inhibited up to 70% of IgE binding to rBet v 1.0101, confirming the specific IgE recognition of the grafted regions. The Bet v 1-specific IgE response is polyclonal, and epitopes are spread across the entire Bet v 1 surface. Furthermore, the IgE recognition profile of Bet v 1 is highly patient specific. Copyright © 2014 The Authors. Published by Mosby, Inc. All rights reserved.

  2. Identification of IgE- binding pollen protein from Cannabis sativa in pollen-hypersensitive patients from north Pakistan.

    Science.gov (United States)

    Choudhary, Shazia; Murad, Sheeba; Hayat, Muhammad Qasim; Shakoor, Zahid; Arshad, Muhammad

    2017-01-01

    Cannabis sativa (C.sativa) is well-known for its medicinal, industrial and recreational use. However, allergies in relation to Cannabis sativa (C.sativa) are rarely reported. C. sativa is one of the common weeds found in Pakistan and its pollen grains are common in spring and fall season. Although categorized as an aeroallergen, there are limited number of reports regarding allergenic potential in C. sativa. Therefore, the current study is aimed at exploring the IgE- binding potential among the C. sativa pollen in local pollen allergic patients. Initial screening of C. sativa sensitized individuals was carried out by dot blot from the sera of pollen allergic patients. Proteins from the pollen grains were extracted and resolved on 10% gel. Eight bands were visible on gel however only one protein fragment i.e. of 14KDa size was found to bind to IgE as analyzed through protein gel blot analysis. Strong IgE affinity of a 14 kDa protein fragment from C. sativa pollen extract suggests its allergenic potential. Further study is required to find the exact nature of this protein fragment.

  3. Evaluation of IgE Antibodies to Omalizumab (Xolair®) and Their Potential Correlation to Anaphylaxis.

    Science.gov (United States)

    Baker, Dana L; Nakamura, Gerald R; Lowman, Henry B; Fischer, Saloumeh Kadkhodayan

    2016-01-01

    Omalizumab (Xolair®) is a recombinant humanized monoclonal antibody that selectively binds to human immunoglobulin E (IgE). Omalizumab is used to treat IgE-mediated diseases such as chronic idiopathic urticaria (CIU) and moderate to severe allergic asthma. In pre-marketing clinical trials in patients with asthma, anaphylaxis was reported in 3 of 3,507 (0.1%) patients. In post-marketing spontaneous reports, the frequency of anaphylaxis attributed to omalizumab use was estimated to be at least 0.2% of patients based on an estimated exposure of about 57,300 patients from June 2003 through December 2006. To better understand the risk of anaphylaxis in patients with allergic asthma receiving omalizumab, a post-marketing pharmacosurveillance study was initiated in 2009. As part of this study, an assay was developed to detect antibodies of IgE isotype to omalizumab. Serum samples from patients in the study were evaluated using this assay. Our results indicated that there was no observable correlation between either anaphylaxis or skin test reactivity and the presence of antibodies of IgE isotype to omalizumab. Here, we discuss the development of this assay as well as the results of the immunogenicity assessment.

  4. IgE- and IgG mediated severe anaphylactic platelet transfusion reaction in a known case of cerebral malaria

    Directory of Open Access Journals (Sweden)

    B Shanthi

    2013-01-01

    Full Text Available Background: Allergic reactions occur commonly in transfusion practice. However, severe anaphylactic reactions are rare; anti-IgA (IgA: Immunoglobulin A in IgA-deficient patients is one of the well-illustrated and reported causes for such reactions. However, IgE-mediated hypersensitivity reaction through blood component transfusion may be caused in parasitic hyperimmunization for IgG and IgE antibodies. Case Report: We have evaluated here a severe anaphylactic transfusion reaction retrospectively in an 18year-old male, a known case of cerebral malaria, developed after platelet transfusions. The examination and investigations revealed classical signs and symptoms of anaphylaxis along with a significant rise in the serum IgE antibody level and IgG by hemagglutination method. Initial mild allergic reaction was followed by severe anaphylactic reaction after the second transfusion of platelets. Conclusion: Based on these results, screening of patients and donors with mild allergic reactions to IgE antibodies may help in understanding the pathogenesis as well as in planning for preventive desensitization and measures for safe transfusion.

  5. Análise da durabilidade de compósitos cimentícios de elevada capacidade de deformação reforçados com fibras

    OpenAIRE

    Fernanda Bianchi Pereira da Costa

    2015-01-01

    Apesar do avanço tecnológico crescente na construção civil, a falta de durabilidade das estruturas de concreto, tanto em edificações como pavimentação, tem sido constatada com acentuada assiduidade e proporção. O compósito cimentício de elevada deformação, também conhecido como Engineered Cementitious Composites (ECC), foi difundido a partir do conceito de concretos de alto desempenho reforçado com fibras, visando suprir o comportamento frágil do concreto convencional e problemas relacionados...

  6. Desarrollo de la tecnología de MOVPE para el crecimiento de semiconductores III-V : fabricación de células solares para concentraciones luminosas elevadas

    OpenAIRE

    Galiana Blanco, Beatriz

    2011-01-01

    El trabajo de estas tesis trata sobre el desarrollo en el IES-UPM de la tecnología de crecimiento epitaxial de fase vapor mediante precursores metalorgánicos (MOVPE) para el crecimiento de células solares de semiconductores III-V para concentraciones luminosas elevadas. En la primera parte de la memoria se presentan los principios de la MOVPE y el estudio de los materiales semiconductores utilizados a lo largo de la tesis: GaAs, AlGaAs, GaInP y AlInP. Para ello, se ha analizado cómo influ...

  7. IgE, IgG4 and IgA specific to Bet v 1-related food allergens do not predict oral allergy syndrome.

    Science.gov (United States)

    Guhsl, E E; Hofstetter, G; Lengger, N; Hemmer, W; Ebner, C; Fröschl, R; Bublin, M; Lupinek, C; Breiteneder, H; Radauer, C

    2015-01-01

    Birch pollen-associated plant food allergy is caused by Bet v 1-specific IgE, but presence of cross-reactive IgE to related allergens does not predict food allergy. The role of other immunoglobulin isotypes in the birch pollen-plant food syndrome has not been investigated in detail. Bet v 1-sensitized birch pollen-allergic patients (n = 35) were diagnosed for food allergy by standardized interviews, skin prick tests, prick-to-prick tests and ImmunoCAP. Concentrations of allergen-specific IgE, IgG1, IgG4 and IgA to seven Bet v 1-related food allergens were determined by ELISA. Bet v 1, Cor a 1, Mal d 1 and Pru p 1 bound IgE from all and IgG4 and IgA from the majority of sera. Immunoglobulins to Gly m 4, Vig r 1 and Api g 1.01 were detected in allergy and increased or reduced levels of IgE, IgG1, IgG4 or IgA specific to most Bet v 1-related allergens. Api g 1-specific IgE was significantly (P = 0.01) elevated in celeriac-allergic compared with celeriac-tolerant patients. Likewise, frequencies of IgE (71% vs 15%; P = 0.01) and IgA (86% vs 38%; P = 0.04) binding to Api g 1.01 were increased. Measurements of allergen-specific immunoglobulins are not suitable for diagnosing Bet v 1-mediated plant food allergy to hazelnut and Rosaceae fruits. In contrast, IgE and IgA to the distantly related allergen Api g 1 correlate with allergy to celeriac. © 2014 The Authors. Allergy Published by John Wiley & Sons Ltd.

  8. The absence of serum IgE antibodies indicates non-type 2 disease in young asthmatics.

    Science.gov (United States)

    Tsolakis, N; Malinovschi, A; Nordvall, L; Janson, C; Borres, M P; Alving, K

    2018-06-01

    Atopic asthma is associated with elevated type-2 biomarkers such as fraction of exhaled nitric oxide (FeNO) and blood eosinophil (B-Eos) count. However, increased type 2 markers have also been reported in traditionally defined non-atopic asthma. To determine a clinically useful level of IgE sensitization for ruling out type 2 asthma. Asthmatics (N = 408; age 10-35 years) were analysed using the multi-allergen tests Phadiatop and fx5 (ImmunoCAP). Subjects were grouped based on IgE-antibody concentrations: ≥0.35 kU A /L for at least one test (n = 326) or <0.35 kU A /L for both tests (n = 82). Τhe latter group was subsequently divided into 2 groups: IgE 0.10-0.34 kU A /L (n = 34) and IgE < 0.10 kU A /L (n = 48). The relationships between type 2 biomarkers, and inadequate asthma control (ACT < 20), reduced lung function (FEV 1  < 80%), recent asthma attacks and airway hyperresponsiveness (AHR) to methacholine were determined. In univariate analyses, at least one type 2 marker related to each asthma outcome in subjects with IgE ≥0.35 kU A /L. In subjects with IgE 0.10-0.34 kU A /L, elevated FeNO related to reduced lung function (P = .008) and B-Eos to AHR (P = .03). No associations were found in subjects with IgE < 0.10 kU A /L. In multivariate analysis, a relationship between FeNO and reduced lung function remained in subjects with IgE < 0.35 kU A /L (P = .03). Clinically relevant elevation of type 2 biomarkers was seen in young asthmatics with IgE antibodies <0.35 kU A /L, but not those with IgE < 0.10 kU A /L. It seems possible to define non-type 2 asthma through sensitive IgE-antibody measurement. © 2018 John Wiley & Sons Ltd.

  9. A novel IgE antibody targeting the prostate-specific antigen as a potential prostate cancer therapy

    International Nuclear Information System (INIS)

    Daniels-Wells, Tracy R; Nicodemus, Christopher F; Penichet, Manuel L; Helguera, Gustavo; Leuchter, Richard K; Quintero, Rafaela; Kozman, Maggie; Rodríguez, José A; Ortiz-Sánchez, Elizabeth; Martínez-Maza, Otoniel; Schultes, Birgit C

    2013-01-01

    Prostate cancer (PCa) is the second leading cause of cancer deaths in men in the United States. The prostate-specific antigen (PSA), often found at high levels in the serum of PCa patients, has been used as a marker for PCa detection and as a target of immunotherapy. The murine IgG1 monoclonal antibody AR47.47, specific for human PSA, has been shown to enhance antigen presentation by human dendritic cells and induce both CD4 and CD8 T-cell activation when complexed with PSA. In this study, we explored the properties of a novel mouse/human chimeric anti-PSA IgE containing the variable regions of AR47.47 as a potential therapy for PCa. Our goal was to take advantage of the unique properties of IgE in order to trigger immune activation against PCa. Binding characteristics of the antibody were determined by ELISA and flow cytometry. In vitro degranulation was determined by the release of β-hexosaminidase from effector cells. In vivo degranulation was monitored in human FcεRIα transgenic mice using the passive cutaneous anaphylaxis assay. These mice were also used for a vaccination study to determine the in vivo anti-cancer effects of this antibody. Significant differences in survival were determined using the Log Rank test. In vitro T-cell activation was studied using human dendritic cells and autologous T cells. The anti-PSA IgE, expressed in murine myeloma cells, is properly assembled and secreted, and binds the antigen and FcεRI. In addition, this antibody is capable of triggering effector cell degranulation in vitro and in vivo when artificially cross-linked, but not in the presence of the natural soluble antigen, suggesting that such an interaction will not trigger systemic anaphylaxis. Importantly, the anti-PSA IgE combined with PSA also triggers immune activation in vitro and in vivo and significantly prolongs the survival of human FcεRIα transgenic mice challenged with PSA-expressing tumors in a prophylactic vaccination setting. The anti-PSA IgE exhibits

  10. pacientes con falla cardiaca

    Directory of Open Access Journals (Sweden)

    Diana Marcela Achury Saldaña

    2007-01-01

    Full Text Available Objetivo: determinar la adherencia al tratamiento de pacientes con falla cardiaca hospitalizados, al aplicar un plan educativo quefomenta el autocuidado.Método: estudio cuasiexperimental (entrevistas enfermera-paciente realizado entre diciembre de 2004 y mayo de 2006, con unamuestra de 50 pacientes seleccionados por conveniencia. Se diseñó un instrumento para evaluar los comportamientos de los pacientes,con base en algunos resultados de la adherencia y sus respectivos indicadores de la taxonomía NOC (Nursing out comes classification. Laadherencia al tratamiento fue medida en dos momentos: el primero durante la hospitalización, seguido de la aplicación del plan educativoantes del alta, que proporcionaba información en el manejo de su enfermedad desde una dimensión física, psicológica y social quepromueve el autocuidado; y el segundo un mes después del alta en su domicilio.Resultados: diferencias estadísticamente significativas (P=0,0001 que demuestran cómo mediante la capacitación al paciente enel manejo de su tratamiento farmacológico y no farmacológico, el establecimiento de una sana relación entre el profesional de enfermeríay el paciente, y la participación de la familia, se logra una total adherencia al tratamiento.Conclusiones: para lograr una adherencia total del paciente con falla cardiaca al tratamiento es necesario un proceso educativo y unseguimiento continuo y personalizado que motive permanentemente al paciente y se le reconozca el papel protagónico en su cuidado y manejo de la enfermedad.

  11. Interleukin-4 and specific IgE to oranges levels study in persons with allergic anamnesis

    Directory of Open Access Journals (Sweden)

    A. S. Prilutskiy

    2014-04-01

    Full Text Available Introduction. Currently allergic diseases pose a global health concern. The connection between immunoglobulin E antibodies (IgE-Ab and allergic disorders is well established. However, the mechanisms through which IgE-Ab contribute to the pathogenesis of allergic diseases are yet to be elucidated. There are reports that highlight the crucial role of interleukins in the regulation of these processes. Thus, some authors pay attention to interleukin-4 (IL-4 which is important for the control of all aspects of the humoral response. Recent epidemiological studies show that oranges are largely consumed worldwide and commonly included in the diet in many countries being one of the main reasons of food allergy. So it was decided to investigate possible links between the levels of IL-4 and IgE-Ab both total and specific to the orange allergens. The aim of this study is to determine the levels of IL-4, total and specific orange IgE-Ab (sIgE-Ab concentrations in individuals with a history of allergic diseases and find some statistically significant links between these parameters. Materials and methods. We selected and investigated serums of 180 patients between 7 months and 78 years with a history of allergic diseases who complained on frequent development of various allergic reactions, mainly atopic dermatitis, urticaria, etc. Determination of the levels of IL-4, total and specific IgE-Ab to the orange allergens was carried out using the first national ELISA test systems of the fourth generation (“Ukrmed-Don”, Donetsk, Ukraine. Specific IgE and IL-4 levels were examined in 180 patients and total IgE levels were investigated in 161 patients. Statistical analysis was performed using the licensed program “MedStat” (Donetsk, Ukraine. The median, the median error, the left and right limits of 95% confidence interval were calculated for all of three samples (IL-4, total IgE-Ab, sIgE-Ab. Central tendencies of two subgroups of IL-4, which were

  12. Positive Skin Test or Specific IgE to Penicillin Does Not Reliably Predict Penicillin Allergy.

    Science.gov (United States)

    Tannert, Line Kring; Mortz, Charlotte Gotthard; Skov, Per Stahl; Bindslev-Jensen, Carsten

    According to guidelines, patients are diagnosed with penicillin allergy if skin test (ST) result or specific IgE (s-IgE) to penicillin is positive. However, the true sensitivity and specificity of these tests are presently not known. To investigate the clinical relevance of a positive ST result and positive s-IgE and to study the reproducibility of ST and s-IgE. A sample of convenience of 25 patients with positive penicillin ST results, antipenicillin s-IgE results, or both was challenged with their culprit penicillin. Further 19 patients were not challenged, but deemed allergic on the basis of a recent anaphylactic reaction or delayed reactions to skin testing. Another sample of convenience of 18 patients, 17 overlapping with the 25 challenged, with initial skin testing and s-IgE (median, 25; range, 3-121), months earlier (T -1 ), was repeat skin tested and had s-IgE measured (T 0 ), and then skin tested and had s-IgE measured 4 weeks later (T 1 ). Only 9 (36%) of 25 were challenge positive. There was an increased probability of being penicillin allergic if both ST result and s-IgE were positive at T 0 . Positive ST result or positive s-IgE alone did not predict penicillin allergy. Among the 18 patients repeatedly tested, 46.2% (12 of 25) of positive ST results at T -1 were reproducibly positive at T 0 . For s-IgE, 54.2% (14 of 24) positive measurements were still positive at T 0 and 7 converted to positive at T 1 . The best predictor for a clinically significant (IgE-mediated) penicillin allergy is a combination of a positive case history with simultaneous positive ST result and s-IgE or a positive challenge result. Copyright © 2017 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  13. Comparative study of specific IgE for cockroach between asthma and allergic rhinitis patients

    International Nuclear Information System (INIS)

    Guo Yinshi; Xu Yiping; Zhu Lijun; Wang Limin; Cao Lingxian; Yao Suhang

    2005-01-01

    To compare the degrees of allergic reaction and the cross-reactive allergens for three strains of cockroach (Periplanceta fuliginosa , Periplaneta americana and Blattella germanica) between patients with asthma and allergic rhinitis, the specific IgE(sIgE) in asthma and allergic rhinitis for these three strains of cockroach were determined with ELISA. The results showed that the sIgE positive rates for Periplaneta americana, Periplaneta fuliginosa and Blattella germanica in patients with asthma were 23.5%, 16.0% and 14.8%, respectively. The reactive coincidence rate between Periplaneta americana and Periplaneta fuliginoas was 74.0%, between Periplaneta americana and Blattella germanica was 73.5%, and between Periplaneta fuliginosa and Blattella germanica was 85.0% in asthma patients. The IgE positive rates for Periplaneta americana, Periplaneta fuliginosa and Blattella gerraanica in allergic rhinitis patients were 24.8%, 17.6% and 15.8%, respectively. The reactive coincidence rate between Periplaneta americana and Periplaneta fuliginosa was 73.9%, between Periplaneta americana and Blattella germanica was 75.2%, and between Periplaneta fuliginosa and Blattella germanica was 86.1% in allergic rhinitis patients. There was no significant difference between asthma and allergic rhinitis patients although the sIgE positive rates of allergic rhinitis patients were higher than those of asthma patients for these three strains of cock- roach. All these results indicated that the degrees of allergic reaction are similar between asthma and allergic rhinitis patients and there are some cross-reactive allergic components among these three strains of cockroach. (authors)

  14. IgE and allergen-specific immunotherapy-induced IgG4 recognize similar epitopes of Bet v 1, the major allergen of birch pollen.

    Science.gov (United States)

    Groh, N; von Loetzen, C S; Subbarayal, B; Möbs, C; Vogel, L; Hoffmann, A; Fötisch, K; Koutsouridou, A; Randow, S; Völker, E; Seutter von Loetzen, A; Rösch, P; Vieths, S; Pfützner, W; Bohle, B; Schiller, D

    2017-05-01

    Allergen-specific immunotherapy (AIT) with birch pollen generates Bet v 1-specific immunoglobulin (Ig)G 4 which blocks IgE-mediated hypersensitivity mechanisms. Whether IgG 4 specific for Bet v 1a competes with IgE for identical epitopes or whether novel epitope specificities of IgG 4 antibodies are developed is under debate. We sought to analyze the epitope specificities of IgE and IgG 4 antibodies from sera of patients who received AIT. 15 sera of patients (13/15 received AIT) with Bet v 1a-specific IgE and IgG 4 were analyzed. The structural arrangements of recombinant (r)Bet v 1a and rBet v 1a _11x , modified in five potential epitopes, were analyzed by circular dichroism and nuclear magnetic resonance spectroscopy. IgE binding to Bet v 1 was assessed by ELISA and mediator release assays. Competitive binding of monoclonal antibodies specific for Bet v 1a and serum IgE/IgG 4 to rBet v 1a and serum antibody binding to a non-allergenic Bet v 1-type model protein presenting an individual epitope for IgE was analyzed in ELISA and western blot. rBet v 1a _11x had a Bet v 1a - similar secondary and tertiary structure. Monomeric dispersion of rBet v 1a _11x was concentration and buffer-dependent. Up to 1500-fold increase in the EC 50 for IgE-mediated mediator release induced by rBet v 1a _11x was determined. The reduction of IgE and IgG 4 binding to rBet v 1a _11x was comparable in 67% (10/15) of sera. Bet v 1a-specific monoclonal antibodies inhibited binding of serum IgE and IgG 4 to 66.1% and 64.9%, respectively. Serum IgE and IgG 4 bound specifically to an individual epitope presented by our model protein in 33% (5/15) of sera. Patients receiving AIT develop Bet v 1a-specific IgG 4 which competes with IgE for partly identical or largely overlapping epitopes. The similarities of epitopes for IgE and IgG 4 might stimulate the development of epitope-specific diagnostics and therapeutics. © 2016 John Wiley & Sons Ltd.

  15. Cementos con cenizas volantes

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    Ossa M., Mauricio

    1984-03-01

    additions of 20 and 30% .

    Casi la generalidad de los estudios realizados sobre cementos con adición de cenizas volantes se refieren a sus características y comportamiento en pastas, morteros y hormigones, siempre en relación con aquéllos del cemento portland. Esta vez, se desarrolló un trabajo experimental orientado a relacionar entre sí los cementos con adiciones de cenizas volantes y de puzolana natural. Para ello se fabricaron a escala de laboratorio cementos de ambos tipos, empleando como materias primas comunes clinker y yeso y, como variables, diferentes porcentajes de las dos adiciones, que cumplieron previamente los requisitos normalizados en cuanto a sus actividades puzolánicas. La calidad de los cementos fabricados resultó adecuada y concordante con la del cemento portland-puzolánico obtenido a escala industrial con los mismos clinker, yeso y puzolana natural de este estudio. Posteriormente, se determinaron las características de los cementos experimentales y se confeccionaron morteros normales para la realización de ensayos físicos y mecánicos. Los resultados de ensayos indicaron que los cementos con adición de cenizas volantes (CCV requieren menos agua para consistencia normal, presentan tiempos de fraguado mayores y expansiones en autoclave menores que los cementos con adición de puzolana (CP. Los calores de hidratación a 7 y 28 días de edad fueron aproximadamente similares para ambos tipos de cemento. En morteros normales, los cementos CCV mostraron menor retracción de secado, mayor retentividad y mayor fluidez (para igual cantidad de agua que los cementos CP. En los ensayos de exudación se observó que ésta depende más de la finura que el tipo de adición. Finalmente, los ensayos mecánicos señalaron que las resistencias a compresión y flexotracción de los morteros con cementos CCV son menores a edades inferiores que 14 días (del orden de 5 a 10% a un día de edad, pero que a partir de entonces pasan a ser mayores que las de

  16. LA ELEVADA PREVALENCIA Y SEVERIDAD DE SÍNTOMAS UROGENITALES DE MUJERES INDÍGENAS ZENÚES COLOMBIANAS EN POSTMENOPAUSIA, TAMBIÉN ES EVIDENCIADA EN OTRA POBLACIÓN AMERINDIA*

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    Álvaro Monterrosa Castro

    2010-12-01

    .8 ± 2.4vs 3.2 ± 1.7, p<0.001, pero ambos grupos tienen una similar y fuerte sintomatología urogenital (6.1 ± 1.6 vs. 6.2 ± 1.4, p>0.05. Las molestias urogenitales, aparecen precozmente en ambos grupos étnicos indígenas y comprometen de forma severa a más del 90% de las mujeres que están en el rango de los 45 – 49 años de edad, magnitud muy superior a la descrita en otros lugares del mundo, y mucho más alta que la obtenida en poblaciones colombianas afro descendientes y mestizas o hispánicas cartageneras urbanas.

    Conclusión: Las mujeres indígenas en posmenopausia de las dos poblaciones estudiadas tienen una prevalencia alta de síntomas precoces relacionados con la dimensión urogenital y manifi estan una presencia de problemas sexuales en igual magnitud. La prevalencia elevada de síntomas urogenitales y su severidad son los que impactan con mayor fuerza en la mala calidad de vida que se observa en las mujeres indígenas, la cual está más deteriorada que en otras etnias colombianas. Los hallazgos obtenidos en la población quechua solidifican los datos ya obtenidos de la población Zenú. Deben valorarse otras etnias amerindias colombianas y latinoamericanas, para globalizar aun más los resultados y puntualizar las diferencias que en términos de síntomas de menopausia, conlleva la etnia/raza.

    Palabras clave: Menopausia. Menopause Rating Scale. Calidad de vida. Climaterio. Indígenas. Latinoamérica. Etnias. Razas.

    Abstract

    The High Prevalence and Severity of Urogenital Symptoms in Postmenopausal Colombian Indigenous Women of Zenú Ethnicity is Also Evident In Other Amerindian Population

    Background: Latin American women have more severe menopausal symptoms than women from other regions of the world. Women of Colombian indigenous groups have more impairment of the urogenital dimension than women of both African and mixed racial ancestry from urban area of

  17. Staphylococcal superantigen-specific IgE antibodies: degree of sensitization and association with severity of asthma.

    Science.gov (United States)

    Elabras, José; Mello, Fernanda Carvalho de Queiroz; Lupi, Omar; Bica, Blanca Elena Rios Gomes; Papi, José Angelo de Souza; França, Alfeu Tavares

    2016-01-01

    To determine the presence of staphylococcal superantigen-specific IgE antibodies and degree of IgE-mediated sensitization, as well as whether or not those are associated with the severity of asthma in adult patients. This was a cross-sectional study involving outpatients with asthma under treatment at a tertiary care university hospital in the city of Rio de Janeiro, Brazil. Consecutive patients were divided into two groups according to the severity of asthma based on the Global Initiative for Asthma criteria: mild asthma (MA), comprising patients with mild intermittent or persistent asthma; and moderate or severe asthma (MSA). We determined the serum levels of staphylococcal toxin-specific IgE antibodies, comparing the results and performing a statistical analysis. The study included 142 patients: 72 in the MA group (median age = 46 years; 59 females) and 70 in the MSA group (median age = 56 years; 60 females). In the sample as a whole, 62 patients (43.7%) presented positive results for staphylococcal toxin-specific IgE antibodies: staphylococcal enterotoxin A (SEA), in 29 (20.4%); SEB, in 35 (24.6%); SEC, in 33 (23.2%); and toxic shock syndrome toxin (TSST), in 45 (31.7%). The mean serum levels of IgE antibodies to SEA, SEB, SEC, and TSST were 0.96 U/L, 1.09 U/L, 1.21 U/L, and 1.18 U/L, respectively. There were no statistically significant differences between the two groups in terms of the qualitative or quantitative results. Serum IgE antibodies to SEA, SEB, SEC, and TSST were detected in 43.7% of the patients in our sample. However, neither the qualitative nor quantitative results showed a statistically significant association with the clinical severity of asthma. Determinar a presença de anticorpos IgE específicos para superantígenos estafilocócicos e o grau de sensibilização mediada por esses, assim como se esses estão associados à gravidade da asma em pacientes adultos. Estudo transversal incluindo asmáticos adultos em acompanhamento ambulatorial em

  18. COMPORTAMIENTO REFRACTARIO DE PROBETAS DE MORTEROS CONFORMADAS CON DIATOMITAS COMO AGREGADO MINERAL

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    Ana Rodriguez

    2008-03-01

    Full Text Available El objetivo de este trabajo es el estudio de las propiedades puzolánicas de algunos minerales, como las diatomitas, que potencialmente pueden reaccionar con la portlandita contenida en las pastas y morteros de cemento Portland. También se estudia si estas adiciones minerales pueden incrementar la resistencia térmica y la refractariedad de los morteros. En el comportamiento de estos agregados se sigue midiendo como parámetro el comportamientopuzolánico de las diatomitas en las pastas de cemento Portland, y las variaciones de la resistencia mecánica de probetas estandarizadas construidas con y sin agregado de diatomitas. El desarrollo experimental programado consta de tratamientostérmicos a las diatomitas, reducción de tamaño hasta compatibilidad con la granulometría del cemento Portland, ensayos de DRX, análisis de puzolanidad, construcción de probetas de morteros con y sinagregado, y finalmente la medición de sus propiedades mecánicas residuales después de haber sido sometidas a elevadas temperaturas.Del análisis de los resultados experimentales se puede informar que las diatomitas estudiadas si le confieren propiedadespuzolánicas al cemento Portland, y como consecuencia mejoran la resistencia mecánica residual de las probetas de mortero cuando son sometidas a altas temperaturas.

  19. Cierre cognitivo, sexismo y religiosidad: diferencias y similitudes entre grupos de adolescentes con distinta cultura

    Directory of Open Access Journals (Sweden)

    Manuel Moyano

    2013-05-01

    Full Text Available Desde el ámbito de la cognición social motivada, la necesidad de cierre cognitivo emerge como una variable crucial para la mejor comprensión de las diferencias individuales en variables psicosociales asociadas a la cultura y, además, susceptible de tener en cuenta en programas de intervención cuyo objetivo vaya dirigido a modificar actitudes ancladas en el prejuicio y la discriminación. En la presente investigación se ha realizado un estudio de este constructo motivacional y su relación con el sexismo y la religiosidad en una muestra de adolescentes cristianos y musulmanes, todos ellos residentes en un contexto social con un marcado carácter multicultural. De acuerdo con los datos obtenidos, no se han encontrado diferencias entre musulmanes y cristianos en necesidad de cierre cognitivo y sexismo. Sin embargo, se han encontrado diferencias por sexo en sexismo hostil. La necesidad de cierre cognitivo correlacionó de una forma estadísticamente significativa con el sexismo (hostil y benevolente, y es un buen predictor. Los musulmanes puntuaron de una forma significativamente más elevada que los cristianos en los indicadores de religiosidad. Se discuten los resultados en el marco de investigaciones previas.

  20. Estudio de la oxidación a elevada temperatura de ocho aleaciones en atmósferas de combustión de biomasa

    Directory of Open Access Journals (Sweden)

    Berlanga-Labari, C.

    2008-08-01

    Full Text Available The combustion of biomass, especially wheat straw, generates very corrosive media particularly near the superheater tubes. Chlorine is known to be the main corrosive agent in biomass-fired plants. This paper focuses on the study of the corrosivity of such environments composed of gases and ashes, at high temperature, on eight commercial alloys with different chromium and nickel contents. These have been: AISI 347 CG (1.4550, AISI 347 FG(1.4550, AISI 316LN (1.4910, ESSHETE 1250 (1.4982, AC 66 (1.4877, X20CrMoV12:1 (1.4922 and AISI 347 coated with Inconel 625 and Inconel 686. Corrosion tests in the boiler have been performed during 8.000 h and 15.000 h at temperatures between 500 °C y 550 °C in the biomass plant of Sangüesa (Navarra, Spain. Experimental results showed an unacceptable behaviour far the martensitic steel, acceptable far the austenitics and good far the inconels. The appearance of the corrosion attack was also investigated to obtain understanding of the corrosion mechanism. The corrosion mechanism by molten salts has been rejected. The active oxidation corrosion mechanism has been proposed as the most probable in order to interpretate the corrosion behaviour of these materials in these environments.

    La combustión de biomasa, especialmente de paja de cereal, genera un ambiente muy corrosivo, particularmente, cerca de los tubos sobrecalentadores. El cloro es el principal agente corrosivo en las plantas de biomasa. Este trabajo se centra en comprobar el comportamiento de ocho aleaciones comerciales en una ambiente de combustión de biomasa. Los materiales estudiados han sido las siguientes: AISI 347 CG (1.4550, AISI 347 FG (1.4550, AISI 316LN (1.4910, ESSHETE 1250 (1.4982, AC 66 (1.4877, X20CrMoV12:1 (1.4922 y AISI 347 recubiertos con Inconel 625 e Inconel 686. Los ensayos se han realizado en la caldera de la planta de biomasa de Sangüesa (Navarra, España durante 8.000 y 15.000 h a una temperatura de 500-550 °C. Los

  1. INSUFICIENCIA RENAL AGUDA CON UREMIA NORMAL EN PACIENTE MONO-RENO SECUNDARIA A PIELONEFRITIS AGUDA

    Directory of Open Access Journals (Sweden)

    Musso CG

    2006-03-01

    Full Text Available RESUMEN:La insuficiencia renal aguda es un sindrome que característicamente cursa con niveles plasmáticos elevados de urea y creatinina. Sin embargo, hay situaciones clínicas en las cuales este sindrome puede cursar con un incremento de la creatininemia sin presentar elevación de la uremia.En este reporte presentamos un caso clínico de una insuficiencia renal aguda con uremia normal secundaria a una pielonefritis aguda en un paciente con riñón único. El paciente presentaba una elevada excreción fraccional de urea lo cual podía explicar su uremia normal pese a estar cursando una caída del filtrado gomerular. Dicha excreción de urea elevada fue interpretada como secundaria a una diabetes insipida nefrogénica y una alteración en el recirculado intra-renal de la urea ambos producto de la pielonefritis aguda. Concluimos que la pielonefritis aguda en un paciente mono-reno puede presentarse con un patrón de insuficiencia renal aguda con uremia normal. SUMMARYAcute renal failure is a syndrome that usually runs with an increase in creatinine and urea plasma levels. However, there are clinical situations in which this syndrome may run with an increase in plasma creatinine keeping normal the urea one.In this report we present a case of acute renal failure with normal plasma urea level secondary to an acute pyelonephritis in a single kidney patient. The patient had an increased fractional excretion of urea which could explain the normal plasma urea levels found despite of his reduced glomerular filtration. This increased urea excretion state was interpreted as a consequence of the nephrogenic diabetes insipidus and alteration of the intra-renal urea reciclying process that the acute pyelonephritis induced. In conclusion: Acute pyelonephritis in a single kidney patient can appear as a pattern of acute renal failure with normal plasma urea levels.

  2. INSUFICIENCIA RENAL AGUDA CON UREMIA NORMAL EN PACIENTE MONO-RENO SECUNDARIA A PIELONEFRITIS AGUDA

    Directory of Open Access Journals (Sweden)

    Musso CG

    2007-04-01

    Full Text Available RESUMENLa insuficiencia renal aguda es un sindrome que característicamente cursa con niveles plasmáticos elevados de urea y creatinina. Sin embargo, hay situaciones clínicas en las cuales este sindrome puede cursar con un incremento de la creatininemia sin presentar elevación de la uremia. En este reporte presentamos un caso clínico de una insuficiencia renal aguda con uremia normal secundaria a una pielonefritis aguda en un paciente con riñón único. El paciente presentaba una elevada excreción fraccional de urea lo cual podía explicar su uremia normal pese a estar cursando una caída del filtrado gomerular. Dicha excreción de urea elevada fue interpretada como secundaria a una diabetes insipida nefrogénica y una alteración en el recirculado intra-renal de la urea ambos producto de la pielonefritis aguda. Concluimos que la pielonefritis aguda en un paciente mono-reno puede presentarse con un patrón de insuficiencia renal aguda con uremia normal.SUMMARYAcute renal failure is a syndrome that usually runs with an increase in creatinine and urea plasma levels. However, there are clinical situations in which this syndrome may run with an increase in plasma creatinine keeping normal the urea one. In this report we present a case of acute renal failure with normal plasma urea level secondary to an acute pyelonephritis in a single kidney patient. The patient had an increased fractional excretion of urea which could explain the normal plasma urea levels found despite of his reduced glomerular filtration. This increased urea excretion state was interpreted as a consequence of the nephrogenic diabetes insipidus and alteration of the intra-renal urea reciclying process that the acute pyelonephritis induced. In conclusion: Acute pyelonephritis in a single kidney patient can appear as a pattern of acute renal failure with normal plasma urea levels.

  3. competencia con China

    Directory of Open Access Journals (Sweden)

    Elena de la Paz Hernández Águila

    2007-01-01

    Full Text Available El artículo ofrece un diagnóstico del desempeño de la industria mexicana del calzado desde la década de los ochenta hasta la actualidad. Analiza la problemática que ha enfrentado esta rama industrial a partir del proceso de apertura comercial y de la competencia en su mercado interno con productos provenientes de países asiáticos, particularmente China. Problematiza al respecto los retos y las perspectivas que a mediano plazo enfrentará este sector empresarial y sobre las posibilidades de competir en el mercado globalizado.

  4. Construir con Madera

    OpenAIRE

    Olabe-Velasco, F. (Fermín); Val-Hernández, Y. (Yolanda); Varela-de-la-Cruz, P. (Perla); Cabrero-Ballarín, J.M. (José Manuel)

    2010-01-01

    Guía divulgativa ‘Construir con madera’, elaborada por la Cátedra Madera de la Universidad de Navarra y el Gobierno de Navarra. La publicación pretende explicar de forma sencilla los beneficios y posibilidades de este material en la construcción, tanto en lo que respecta a su resistencia, comportamiento frente al fuego, durabilidad, capacidad de aislamiento, propiedades acústicas, estética, respeto al medio ambiente y sostenibilidad como fuente de energía. A modo de ejemplo, en la ...

  5. Agranulocitosis inducida por metimazol en pacientes con enfermedad de Graves

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    Helard Manrique-Hurtado

    2013-04-01

    Full Text Available Objetivo: Describir las características clínicas y epidemiológicas de los pacientes con enfermedad de Graves que presentaron agranulocitosis inducida por metimazol. Material y métodos: Estudio retrospectivo, tipo serie de casos. Se revisaron las historias clínicas de todos los pacientes con diagnóstico de agranulocitosis inducida por metimazol, atendidos en el Hospital Nacional Arzobispo Loayza, entre enero 2002 y diciembre 2008. Se buscó asociación entre las variables demográficas y clínicas con la mortalidad y el tiempo de recuperación. Resultados: Treinta (0,60% pacientes con enfermedad de Graves fueron hospitalizados con el diagnóstico de agranulocitosis inducida por metimazol. La mediana de la edad fue 33,5 años y 86,67% fueron mujeres. Al ingreso, todos los pacientes presentaron fiebre y dolor de garganta. El manejo incluyó aislamiento invertido, suspensión del metimazol, administración de antibióticos y glucocorticoides. Doce (40% pacientes recibieron GM-CSF. El número de granulocitos se normalizó después de 10,59 días y cuatro (13,33% pacientes murieron por infecciones bacterianas y sepsis. En todos los casos, el tratamiento definitivo fue yodo radioactivo. No hubo diferencia significativa en la edad, sexo, dosis de metimazol, duración del tratamiento y uso de factor estimulante colonia, entre los pacientes fallecidos y los sobrevivientes. Además, el uso de factor estimulante de colonia no redujo el tiempo de recuperación de la agranulocitosis. Conclusión: La agranulocitosis inducida por metimazol es un evento adverso serio y potencialmente mortal. En este grupo de pacientes, la mortalidad fue elevada y el uso de factor estimulante de colonia no disminuyó el tiempo de recuperación.

  6. Remoción de Arsenico (V) y cromo (VI) presente en lechos acuáticos mediante el uso de tierra de diatomeas recubierta con sales de hierrro Arequipa 2016

    OpenAIRE

    Rojas Tamata, Karen

    2016-01-01

    RESUMEN La región de Arequipa cuenta con recursos naturales minerales y no minerales con mucho potencial comercial, entre ellas se destaca a la tierra de diatomeas, el 23% de las diatomeas del Perú están en nuestra región; por la naturaleza del terreno volcánico la presencia de arsénico es inminente en el agua, además existen empresas dedicadas a la curtiembre del cuero que eliminan sus desechos sin ningún otro tipo de tratamiento, por lo cual existen concentraciones elevadas de dichos m...

  7. Entrevista con Giovanni Levi

    Directory of Open Access Journals (Sweden)

    Monica Oliveira

    2017-06-01

    Full Text Available En esta entrevista, Giovanni Levi - como un conocedor del tema de Familia - realiza una importante evaluación sobre el actual estado de las investigaciones realizadas en el Brasil y em el exterior. Con estilo franco, agudo y lucido critica las visiones tradicionales y sus ilusiones ypropone nuevos conceptos y métodos. La historia de la familia debería ceder espacio para el estudio de las redes relacionales o de los mundos relacionales. De la misma forma, la historia cuantitativa debería abrir espacio para el estudio de las cualidades. Ya con relación a la historia de las elites, tan estudiada y reproducida en una diversidad de trabajos, que deberíase mirar en otra perspectiva. Es decir, no mirar a las reglas sociales predeterminadas, sino a los desvíos y a las variaciones. Levi defiende que los historiadores deben trascender a los documentos que se encuentran fácilmente y que pueden fortalecer perspectivas deformadas y esequilibradas de la sociedad. Para él, los historiadores deben esforzarse por estudiar a aquellos grupos que dejaron pocos rastros documentales. En ese esfuerzo existiría una nueva mirada sobre la historia de la familia.

  8. Entrevista con Patricia Ariza

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    Esperanza Londoño La Rotta

    2017-01-01

    Full Text Available Pensamiento, Palabra y Obra entrevista a una artista, feminista y activista política, quien como mujer y artista ha permitido pensar el arte más allá de un simple espectáculo. Toda una vida dedicada al teatro y a darle voz, a través de sus obras, a víctimas del conflicto colombiano, defensora de derechos humanos; además de hacer evidente en su vida y a través de la plataforma “Artistas por la paz”, las múltiples relaciones que se pueden establecer entre el arte, la construcción de paz y la resolución de conflictos. Hablamos en su casa, en medio del calor de la bienvenida con Patricia Ariza, directora del festival alternativo de teatro, de Mujeres en Escena y de la Corporación Colombiana de Teatro, entre otras muchas actividades que voluntariamente su espíritu libertario ha asumido. Esta entrevista se realizó antes del 2 de octubre, pero con la revisión de los acuerdos que propició el plebiscito ganado por una ínfima minoría por el no, sigue siendo vigente este planteamiento.

  9. Modulation of the allergen-induced human IgE response in Hu-SCID mice: inhibitory effect of human recombinant IFN-gamma and allergen-derived lipopeptide.

    Science.gov (United States)

    Duez, C; Gras-Masse, H; Hammad, H; Akoum, H; Didierlaurent, A; André, C; Tonnel, A B; Pestel, J

    2001-01-01

    We have previously established a model to study the in vivo human IgE response using humanized SCID mice. Allergic SCID mice were obtained following intraperitoneal injection with mononuclear cells from Dermatophagoides pteronyssinus (Dpt)-sensitive patients, and sensitization by Dpt allergen intraperitoneal injection (immunization) or Dpt aerosol (inhalation). Human serum IgE was measured in allergic SCID mice after administration of human recombinant IFN-gamma or the lipopeptide LP 52-71 (derived from peptide p52-71 from Der p 1, Dpt major allergen, coupled to a lipophilic moiety), during the immunization or the inhalation phase. IFN-gamma inhibited human IgE production when given at the time of immunization, but not during inhalation. This effect was long-lasting as Dpt aerosol, given one month after immunization and IFN-gamma administration, failed to increase IgE levels. Unlike Dpt or p52-71, LP 52-71 failed to induce human IgE production at day 14 and 21 after its injection, but did inhibit the development of the IgE response after a secondary Dpt-challenge. Moreover, LP 52-71 administration 14 days after Dpt inhalation decreased IgE levels, in contrast to peptide 52-71, which increased IgE levels. Thus, taken together these results indicate that the development of the human IgE response in allergic SCID mice can be modulated by modified allergen and a Th1 cytokine.

  10. Cutaneous cytomegalovirus infection in a child with hyper IgE and specific defects in antibody response to protein vaccines

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    Shahrzad Fallah

    Full Text Available Cytomegalovirus (CMV infection is a common opportunistic systemic infection in immunocompromised patients, but skin involvement is rare. Herein, we report a 10 year-old girl from consanguineous parents who was referred to our center because of disseminated maculopapular rash. She had history of upper and lower respiratory tract infections. In immunological studies, increased serum IgE level and decreased responses to tetanus and diphtheria were detected. Polymerase chain reaction (PCR examination of bronchoalveolar lavage and serum sample revealed the presence of CMV. Early diagnosis of cutaneous CMV and appropriate treatment are the key actions in management of patients with underlying immunodeficiencies to avoid further complications.

  11. Approaches to assess IgE mediated allergy risks (sensitization and cross-reactivity) from new or modified dietary proteins

    DEFF Research Database (Denmark)

    Remington, B.; Broekman, H. C. H.; Blom, W. M.

    2018-01-01

    for new proteins, and especially to identify and characterise the risk of sensitization for IgE mediated allergy from oral exposure. Existing tools and tests are capable of assessing potential crossreactivity. However, there are few possibilities to assess the hazard due to de novo sensitization. The only...... methods available are in vivo models, but many limitations exist to use them for assessing risk. We conclude that there is a need to understand which criteria adequately define allergenicity for risk assessment purposes, and from these criteria develop a more suitable battery of tests to distinguish...

  12. IgE recognition of chimeric isoforms of the honeybee (Apis mellifera) venom allergen Api m 10 evaluated by protein array technology.

    Science.gov (United States)

    Van Vaerenbergh, Matthias; De Smet, Lina; Rafei-Shamsabadi, David; Blank, Simon; Spillner, Edzard; Ebo, Didier G; Devreese, Bart; Jakob, Thilo; de Graaf, Dirk C

    2015-02-01

    Api m 10 has recently been established as novel major allergen that is recognized by more than 60% of honeybee venom (HBV) allergic patients. Previous studies suggest Api m 10 protein heterogeneity which may have implications for diagnosis and immunotherapy of HBV allergy. In the present study, RT-PCR revealed the expression of at least nine additional Api m 10 transcript isoforms by the venom glands. Two distinct mechanisms are responsible for the generation of these isoforms: while the previously known variant 2 is produced by an alternative splicing event, novel identified isoforms are intragenic chimeric transcripts. To the best of our knowledge, this is the first report of the identification of chimeric transcripts generated by the honeybee. By a retrospective proteomic analysis we found evidence for the presence of several of these isoforms in the venom proteome. Additionally, we analyzed IgE reactivity to different isoforms by protein array technology using sera from HBV allergic patients, which revealed that IgE recognition of Api m 10 is both isoform- and patient-specific. While it was previously demonstrated that the majority of HBV allergic patients display IgE reactivity to variant 2, our study also shows that some patients lacking IgE antibodies for variant 2 display IgE reactivity to two of the novel identified Api m 10 variants, i.e. variants 3 and 4. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. Relación del índice de masa corporal con felicidad, autoestima y depresión en adultos jóvenes

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    Graciela Cristina Avitia

    2018-01-01

    Full Text Available La obesidad es una enfermedad con repercusión psicológica, por lo que es importante reconocer los rasgos psicológicos y de comportamiento de quienes la padecen. El objetivo de este trabajo es analizar los niveles de felicidad, autoestima y depresión en individuos con normopeso (NP y con sobrepeso/obesidad (SO. La muestra estuvo constituida por 151 alumnos de la Universidad Autónoma de Ciudad Juárez (UACJ con una media de edad de 21.44 años. Los resultados mostraron que el grupo de SO tuvo puntuaciones significativamente más elevadas en sentido positivo de la vida y realización personal, en comparación con el grupo de NP. Sin embargo, no se encontraron diferencias significativas en autoestima y depresión. Estos resultados no coinciden con los de otras investigaciones y podrían ser explicados por el grado de aceptación en el entorno social hacia las personas con obesidad, las estrategias de afrontamiento y la prevalencia de obesidad y sobrepeso entre otras variables con las que se ha asociado la relación obesidad/infelicidad.

  14. Analgesia postoperatoria con catéter incisional en cesáreas electivas

    OpenAIRE

    Rodríguez Morejón, María del Carmen

    2012-01-01

    La cesárea supone hoy en día una de las intervenciones quirúrgicas obstétricas más frecuentes. A esto se añade que la cesárea está dentro del grupo de intervenciones con un dolor postoperatorio de intensidad elevada y que ninguna opción analgésica ha demostrado ser la mejor. Por ello se diseñó un estudio de intervención, prospectivo, aleatorizado, no ciego, para comparar la eficacia, en el control del dolor tras la cesárea (medido mediante Escala Visual Analógica), de la analgesia incision...

  15. Mortality is higher in patients with leptomeningeal metastasis in spinal cord tumors Mortalidade é mais elevada na disseminação metastática leptomeníngea em tumores da medula espinhal

    Directory of Open Access Journals (Sweden)

    Ricardo de Amoreira Gepp

    2012-01-01

    Full Text Available Spinal cord tumors are a rare neoplasm of the central nervous system (CNS. The occurrence of metastases is related to poor prognosis. The authors analyzed one series of metastasis cases and their associated mortality. METHODS: Clinical characteristics were studied in six patients with intramedullary tumors with metastases in a series of 71 surgical cases. RESULTS: Five patients had ependymomas of which two were WHO grade III. The patient with astrocytoma had a grade II histopathological classification. Two patients required shunts for hydrocephalus. The survival curve showed a higher mortality than the general group of patients with no metastases in the CNS (pTumores da medula espinhal são neoplasias raras do sistema nervoso central (SNC. A ocorrência de metástases é relacionada a pior prognóstico. Os autores analisaram uma série de casos de metástases e a mortalidade relacionada. MÉTODO: Foram estudadas as características clínicas em seis pacientes com metástases tumorais numa série de 71 casos operados. RESULTADOS: Cinco pacientes tinham ependimomas e dois dos quais foram grau III pela classificação da OMS. O paciente portador de astrocitoma tinha classificação histopatológica de grau II. Dois pacientes necessitaram de derivação devido à hidrocefalia. A curva de sobrevivência mostrou mortalidade mais elevada no grupo de pacientes com disseminação pelo SNC (p<0,0001. CONCLUSÃO: A mortalidade, além de elevada em pacientes com metástases, é maior do que em pacientes apenas com lesão primária. Os ependimomas, independentemente do seu grau de anaplasia, costumam causar mais metástases do que os astrocitomas medulares.

  16. Mortality is higher in patients with leptomeningeal metastasis in spinal cord tumors Mortalidade é mais elevada na disseminação metastática leptomeníngea em tumores da medula espinhal

    Directory of Open Access Journals (Sweden)

    Ricardo de Amoreira Gepp

    2013-01-01

    Full Text Available Spinal cord tumors are a rare neoplasm of the central nervous system (CNS. The occurrence of metastases is related to poor prognosis. The authors analyzed one series of metastasis cases and their associated mortality. METHODS: Clinical characteristics were studied in six patients with intramedullary tumors with metastases in a series of 71 surgical cases. RESULTS: Five patients had ependymomas of which two were WHO grade III. The patient with astrocytoma had a grade II histopathological classification. Two patients required shunts for hydrocephalus. The survival curve showed a higher mortality than the general group of patients with no metastases in the CNS (pTumores da medula espinhal são neoplasias raras do sistema nervoso central (SNC. A ocorrência de metástases é relacionada a pior prognóstico. Os autores analisaram uma série de casos de metástases e a mortalidade relacionada. MÉTODO: Foram estudadas as características clínicas em seis pacientes com metástases tumorais numa série de 71 casos operados. RESULTADOS: Cinco pacientes tinham ependimomas e dois dos quais foram grau III pela classificação da OMS. O paciente portador de astrocitoma tinha classificação histopatológica de grau II. Dois pacientes necessitaram de derivação devido à hidrocefalia. A curva de sobrevivência mostrou mortalidade mais elevada no grupo de pacientes com disseminação pelo SNC (p<0,0001. CONCLUSÃO: A mortalidade, além de elevada em pacientes com metástases, é maior do que em pacientes apenas com lesão primária. Os ependimomas, independentemente do seu grau de anaplasia, costumam causar mais metástases do que os astrocitomas medulares.

  17. Remoción de mercúrio en lixiviados de un relleno sanitário empleando carbón activado impregnado con azufre

    OpenAIRE

    Ocampo Barrero, Maria Fanny; Londono Carvajal, Adela; Girado Gómez, Gloria Inés; Gonzalez, Nancy Rocio Sanabria

    2017-01-01

    RESUMEN Introducción. Los lixiviados del relleno sanitario La Esmeralda, de Manizales (Colombia) presentan una elevada concentración de mercurio, el cual es un metal altamente tóxico para la salud humana y de los ecosistemas. La adsorción de mercurio en solución sobre carbón activado es un método eficiente para su remoción, y la modificación de la superficie del carbón activado con azufre mejora la capacidad de adsorción de este metal en medio acuoso. Objetivo. Evaluar la remoción de mercur...

  18. Distorsión de la Percepción Corporal en Pacientes con Anorexia Nerviosa de Inicio Temprano vs. Tardío

    OpenAIRE

    María Yolanda Vellisca González; Santos Orejudo Hernández; José Ignacio Latorre Marín

    2012-01-01

    La anorexia nerviosa, como trastorno de la conducta alimentaria, está asociada a una elevada preocupación por el peso, a ideas sobrevaloradas respecto a la delgadez, a una alteración de la imagen corporal y a un miedo excesivo a engordar. En este trabajo estudiamos las diferencias en la distorsión de la percepción corporal en pacientes con anorexia nerviosa de inicio temprano vs. tardío mediante el Cuestionario de la forma corporal (Body Shape Questionnaire) de Cooper, Taylor, Cooper y Fairbu...

  19. Comportamiento térmico y mecánico de mezclas de PC/PET con altos contenidos de PC

    OpenAIRE

    Cornement, Frédéric

    2006-01-01

    Las mezclas de polímeros tienen un interés científico e industrial considerable. Mezclar es una manera muy eficaz para adaptar las propiedades de los polímeros a diferentes circunstancias. El Poli(Etilén Tereftalato) (PET) y el PoliCarbonato (PC) son dos termoplásticos muy importantes. PC tiene una elevada resistencia al impacto y su resistencia a los solventes se mejora cuando se mezcla con PET. Los dos son poliésteres y pueden reaccionar mediante transesterificación en el fundido p...

  20. Determinación de bismuto por flotación de un par iónico con ioduro y rivanol.

    OpenAIRE

    López Erroz, Carmen; Hernández Córdoba, Manuel

    1987-01-01

    El Bi{III) origina complejos aniónicos con ligandos inorgánicos, preferentemente ioduro y tiocianato, que son extraíbles en los disolventes orgánicos. Utilizando esta propiedad se han derivado aplicaciones analíticas para la determinación del ion metálico, generalmente mediante el empleo de la técnica espectrofotométrica. La sensibilidad de estos procedimientos no es elevada, pero puede aumentarse si el complejo aniónico de bismuto se extrae como un compuesto d...

  1. Instellar Gas Experiment (IGE): Testing interstellar gas particles to provide information on the processes of nucleosynthesis in the big bang stars and supernova

    Science.gov (United States)

    Lind, Don

    1985-01-01

    The Interstellar Gas Experiment (IGE) is designed to collect particles of the interstellar gas - a wind of interstellar media particles moving in the vicinity of the solar system. These particles will be returned to earth where the isotopic ratios of the noble gases among these particles will be measured. IGE was designed and programmed to expose 7 sets of six copper-beryllium metallic collecting foils to the flux of neutral interstellar gas particles which penetrate the heliosphere to the vicinity of the earth's orbit. These particles are trapped in the collecting foils and will be returned to earth for mass-spectrographic analysis when Long Duration Exposure Facility (LDEF) on which IGE was launched, is recovered.

  2. Pretreatment IgE sensitization patterns determine the molecular profile of the IgG4 response during updosing of subcutaneous immunotherapy with timothy grass pollen extract

    DEFF Research Database (Denmark)

    Schmid, Johannes Martin; Würtzen, Peter Adler; Dahl, Ronald

    2016-01-01

    BACKGROUND: Allergen immunotherapy is an effective treatment of allergic rhinoconjunctivitis. Clinical efficacy is associated with improvement of basophil sensitivity and an increase in allergen-specific immunoglobulin concentration. OBJECTIVE: We sought to determine whether changes in allergen...... component-specific serum IgE and IgG4 levels during the updosing phase of subcutaneous immunotherapy (SCIT) are biomarkers of the immunologic changes that can lead to treatment efficacy. METHODS: Twenty-four subjects with grass pollen-induced allergic rhinoconjunctivitis were randomized 3:1 to receive SCIT...... (Alutard SQ) or to an open control group. IgE and IgG4 concentrations were determined for the major allergens Phl p 1 or Phl p 5 by using ImmunoCAP and for 8 grass pollen molecules by using Immuno Solid-phase Allergy Chip (ISAC) before treatment and after updosing. RESULTS: Levels of specific IgE against...

  3. Correlation of the severity of atopic dermatitis with absolute eosinophil counts in peripheral blood and serum IgE levels

    Directory of Open Access Journals (Sweden)

    Dhar Sandipan

    2005-01-01

    Full Text Available BACKGROUND: Although a number of epidemiological studies, showing incidence and prevalence of atopic dermatitis, were available, scant attention has been paid to the correlation between the parameters of the disease like severity, absolute eosinophil count and IgE level, which has been known to be associated inconsistently. Hence this study was undertaken. METHODS: A total of 102 patients of atopic dermatitis, both children and adults, and 107 age matched controls were studied at the Pediatric Dermatology clinic, Institute of Child Health and department of Dermatology, AMRI-Apollo hospitals, Kolkata. RESULTS: The average age of onset of atopic dermatitis was observed to be 4.55 years. Both the average absolute eosinophil count and IgE levels in patients of atopic dermatitis were significantly higher than that of the controls. Each of these parameters showed significant correlation with severity of the disease and showed a nonhomogeneous distribution reflected by significant association with personal history of bronchial asthma and family history of atopy, when both parents were atopic. CONCLUSIONS: Our study shows that clinical activity of the disease as recorded by the "SCORAD" index can be used as an indicator of the hematological abnormalities as well as to some extent as a prognostic indicator. Family history of atopy correlates with the hematological abnormalities only if both parents are involved and bronchial asthma is the only associated atopic condition which correlates with the parameters of the disease .

  4. House dust-mite allergen exposure is associated with serum specific IgE but not with respiratory outcomes.

    Science.gov (United States)

    Bakolis, I; Heinrich, J; Zock, J P; Norbäck, D; Svanes, C; Chen, C M; Accordini, S; Verlato, G; Olivieri, M; Jarvis, D

    2015-06-01

    Exposure to house dust has been associated with asthma in adults, and this is commonly interpreted as a direct immunologic response to dust-mite allergens in those who are IgE sensitized to house dust-mite. Mattress house dust-mite concentrations were measured in a population-based sample of 2890 adults aged between 27 and 56 years living in 22 centers in 10 countries. Generalized linear mixed models were employed to explore the association of respiratory symptoms with house dust-mite concentrations, adjusting for individual and household confounders. There was no overall association of respiratory outcomes with measured house dust-mite concentrations, even in those who reported they had symptoms on exposure to dust and those who had physician-diagnosed asthma. However, there was a positive association of high serum specific IgE levels to HDM (>3.5 kUA /l) with mattress house dust-mite concentrations and a negative association of sensitization to cat with increasing house dust-mite concentrations. In conclusion, there was no evidence that respiratory symptoms in adults were associated with exposure to house dust-mite allergen in the mattress, but an association of house mite with strong sensitization was observed. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  5. Performing IgE serum testing due to bioinformatics matches in the allergenicity assessment of GM crops.

    Science.gov (United States)

    Goodman, Richard E

    2008-10-01

    Proteins introduced into genetically modified (GM) organisms through genetic engineering must be evaluated for their potential to cause allergic disease under various national laws and regulations. The Codex Alimentarius Commission guidance document (2003) calls for testing of serum IgE binding to the introduced protein if the gene was from an allergenic source, or the sequence of the transferred protein has >35% identity in any segment of 80 or more amino acids to a known allergen or shares significant short amino acid identities. The Codex guidance recognized that the assessment will evolve based on new scientific knowledge. Arguably, the current criteria are too conservative as discussed in this paper and they do not provide practical guidance on serum testing. The goals of this paper are: (1) to summarize evidence supporting the level of identity that indicates potential risk of cross-reactivity for those with existing allergies; (2) to provide example bioinformatics results and discuss their interpretation using published examples of proteins expressed in transgenic crops; and (3) to discuss key factors of experimental design and methodology for serum IgE tests to minimize the rate of false negative and false positive identification of potential allergens and cross-reactive proteins.

  6. Molecular allergy diagnostics using IgE singleplex determinations: methodological and practical considerations for use in clinical routine: Part 18 of the Series Molecular Allergology.

    Science.gov (United States)

    Kleine-Tebbe, Jörg; Jakob, Thilo

    Allergen molecules (synonyms: single allergens, allergen components) open up new horizons for the targeted allergen-specific diagnostics of immunoglobulin E (IgE) in singleplex determination. The following rationales support the targeted use of allergen molecules and, more importantly, improve test properties: (1) increased test sensitivity ("analytical sensitivity"), particularly when important allergens are under-represented or lacking in the extract; (2) improved test selectivity (analytical specificity), particularly when the selected IgE repertoire against an allergen yields additional information on: (a) potential risk, (b) possible cross-reactivity, or (c) primary (species-specific) sensitization. However, the appropriate indication for the use of single allergens can only be established on a case-by-case basis (depending on the clinical context and previous history) and in an allergen-specific manner (depending on the allergen source and the single allergens available), rather than in a standardized way. Numerous investigations on suspected food allergy, insect venom allergy, or sensitization to respiratory allergens have meanwhile demonstrated the successful use of defined molecules for allergen-specific singleplex IgE diagnosis. Specific IgE to single allergens is limited in its suitability to predict the clinical relevance of sensitivity on an individual basis. In food allergies, one can at best identify the relative risk of a clinical reaction on the basis of an IgE profile, but no absolutely reliable prediction on (future) tolerance can be made. Ultimately, the clinical relevance of all IgE findings depends on the presence of corresponding symptoms and can only be assessed on an individual basis (previous history, symptom log, and provocation testing with the relevant allergen source where appropriate). Thus, also in molecular allergology, the treating physician and not the test result should determine the clinical relevance of diagnostic findings

  7. Serum IgE and IgG4 against muscle larva excretory-secretory products during the early and late phases of human trichinellosis.

    Science.gov (United States)

    Calcagno, Marcela A; Forastiero, María A; Saracino, María P; Vila, Cecilia C; Venturiello, Stella M

    2017-11-01

    In human trichinellosis, the relevance of the presence and persistence of specific serum IgE and IgG4 during the early and late phases of infection is still controversial.The aim of this work was to determine the percentage of human sera presenting IgE and IgG4 against Trichinella spiralis muscle larvae excretory-secretory products as well as their levels during the early and late phases of the infection. The antigen recognition pattern by serum total immunoglobulins (IgGAM), IgE, and IgG4 was assessed over time. Serum samples during early and late phases were analyzed by ELISA and immunoelectrotransfer blot (IETB).Results showed that (a)-IgE and IgG4 are present at constant levels in both phases; (b)-IgE recognized the glycoproteins of ~ 45 and ~ 55 kDa and IgG4 only the ~ 45 kDa; (c)-in the late phase, the percentage of specific IgE positive sera was higher than that of specific IgG4 by IETB; while in serum samples taken during the early phase, no differences were found between both isotypes; (d)-both isotypes displayed different glycoprotein recognition patterns: the pattern corresponding to IgE was coincident with that of IgGAM, comprising seven glycoproteins (ranging from ~ 116 to ~ 29 kDa), whereas IgG4 revealed four glycoproteins (ranging from ~ 97 to ~ 45 kDa), showing a different sera recognition percentage depending on the phase studied.In conclusion, IgE and IgG4 cannot be considered exclusive isotypes of neither the early nor the late phase of infection and they are as useful as the detection of total antibodies in the early diagnosis.

  8. Maternal Genetic Variants of IL4/IL13 Pathway Genes on IgE With "Western or Eastern Environments/Lifestyles".

    Science.gov (United States)

    Zhang, Guicheng; Khoo, Siew-Kim; Mäkelä, Mika J; Candelaria, Pierre; Hayden, Catherine M; von Hertzen, Leena; Laatikainen, Tiina; Vartiainen, Erkki; Goldblatt, Jack; Haahtela, Tari; LeSouëf, Peter N

    2014-07-01

    We investigated maternal genetic effects of four IL-4/IL-13 pathway genes as well as their interactions with the "Western or Eastern lifestyles/environments" on IgE in Karelian children. This study included 609 children and their mothers. Total IgE levels in children and mothers were measured and 10 single nucleotide polymorphisms (SNPs) in IL-4, IL-4Ra, IL-13, and STAT6 were genotyped in mothers and their children. The maternal G allele of IL-13 130 (rs20541) was significantly (P=0.001) associated with decreased IgE in children in the Karelian population (Pooling Finnish and Russian children), as well as in Finnish (P=0.030) and Russian children (P=0.018). The IgE levels were significantly (P=0.001) higher in Russian children whose mothers were homozygous for the G allele of the IL-4Ra 50 (rs1805010) SNP than that in Russian children of mothers who were AG heterozygotes or AA homozygotes. After accounting for children's genotypes, we observed interactive effects on children's IgE for maternal IL-13 130 genotypes (P=0.014) and maternal IL-4Ra 50 genotypes (P=0.0003) with "Western or Eastern" lifestyles/environments. With the adjustment for multiple comparisons using a false discovery rate (FDR) of 0.05, the interactive effect of the maternal IL-4Ra50 SNP was significant. Maternal genetic variants in IL-4/IL-13 pathway genes, such as IL-13 130 and IL-4Ra50, influenced IgE levels in school children that were independent of the children's genetic effects. These effects differ in "Western or Eastern" environments.

  9. Association between the MHC gene region and variation of serum IgE levels against specific mould allergens in the horse

    Directory of Open Access Journals (Sweden)

    Curik Ino

    2003-06-01

    Full Text Available Abstract To investigate whether the equine major histocompatibility complex (MHC gene region influences the production of mould-specific immunoglobulin E antibodies (IgE, alleles of the equine leukocyte antigen (ELA-A locus and three microsatellite markers (UM-011, HTG-05 and HMS-42 located on the same chromosome as the equine MHC were determined in 448 Lipizzan horses. Statistical analyses based on composite models, showed significant associations of the ELA-A and UM-011 loci with IgE titres against the recombinant Aspergillus fumigatus 7 antigen (rAsp f 7. UM-011 was also significantly associated with IgE titres against the recombinant Aspergillus fumigatus 8 antigen (rAsp f 8. In addition to the loci mentioned above, the MHC class II DQA and DRA loci were determined in 76 Lipizzans from one stud. For IgE levels against rAsp f 7, the composite model showed the strongest association for DQA (P rAsp f 8 specific IgE levels, similarly to the results found with all 448 horses, the strongest association was found with UM-011 (P = 0.01, which is closely linked with the MHC class II DRB locus. These results suggest that the equine MHC gene region and possibly MHC class II loci, influence the specific IgE response in the horse. However, although the strongest associations were found with DQA and UM-011, this study did not distinguish if the observed effects were due to the MHC itself or to other tightly linked genes.

  10. Effects of routine prophylactic vaccination or administration of aluminum adjuvant alone on allergen-specific serum IgE and IgG responses in allergic dogs.

    Science.gov (United States)

    Tater, Kathy C; Jackson, Hilary A; Paps, Judy; Hammerberg, Bruce

    2005-09-01

    To determine the acute corn-specific serum IgE and IgG, total serum IgE, and clinical responses to s.c. administration of prophylactic vaccines and aluminum adjuvant in corn-allergic dogs. 20 allergic and 8 nonallergic dogs. 17 corn-allergic dogs were vaccinated. Eight clinically normal dogs also were vaccinated as a control group. Serum corn-specific IgE, corn-specific IgG, and total IgE concentrations were measured in each dog before vaccination and 1 and 3 weeks after vaccination by use of an ELISA. The corn-allergic dogs also had serum immunoglobulin concentrations measured at 8 and 9 weeks after vaccination. Twenty allergic dogs received a s.c. injection of aluminum adjuvant, and serum immunoglobulin concentrations were measured in each dog 1, 2, 3, 4, and 8 weeks after injection. The allergic dogs were examined during the 8 weeks after aluminum administration for clinical signs of allergic disease. The allergic dogs had significant increases in serum corn-specific IgE and IgG concentrations 1 and 3 weeks after vaccination but not 8 or 9 weeks after vaccination. Control dogs did not have a significant change in serum immunoglobulin concentrations after vaccination. After injection of aluminum adjuvant, the allergic dogs did not have a significant change in serum immunoglobulin concentrations or clinical signs. Allergen-specific IgE and IgG concentrations increase after prophylactic vaccination in allergic dogs but not in clinically normal dogs. Prophylactic vaccination of dogs with food allergies may affect results of serologic allergen-specific immunoglobulin testing performed within 8 weeks after vaccination.

  11. IgE mediated food allergy in Korean children: focused on plant food allergy.

    Science.gov (United States)

    Lee, Soo-Young

    2013-01-01

    , we reveals that the peanut sensitization rate in Korea reaches 18%, and that, when sensitized to peanut, patients showed a significant tendency to have co-sensitization with house dust mites, egg white, wheat, and soybean. The higher specific IgE to peanut was related to the likelihood of the patient developing severe systemic reactions. In another study, based on the data analysis of 69 patients under 4 years of age who had suspected peanut and tree nut allergy, 22 (31.9%) were sensitized to walnut (>0.35 kU/L, 0.45-27.4 kU/L) and 6 (8.7%) experienced anaphylaxis due to a small amount of walnut exposure. Furthermore, in this review, clinical and immunological studies on plant food allergies, such as buckwheat allergy, rice allergy, barley allergy, and kiwi fruit allergy, in Korean children are discussed.

  12. Manifestaciones mucocutaneas en pacientes con lupus eritematoso sistemico

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    V. Saurit

    2003-08-01

    Full Text Available El objetivo fue estudiar en pacientes con lupus eritematoso sistémico (LES la prevalencia de las lesiones mucocutáneas. Se incluyeron 77 pacientes. Las lesiones mucocutáneas fueron clasificadas en específicas y no específicas. Se definió el fototipo, exposición solar y fotoprotección. Se relacionaron las lesiones con la serología y la actividad de la enfermedad. Las lesiones específicas encontradas fueron: agudas (67.5%, lupus subagudo (6.3% y lesiones crónicas (26.0%. Las lesiones no específicas fueron: alopecia difusa (59%, fotosensibilidad (57%, fenómeno de Raynaud (47%, úlceras orales (16% y livedo reticularis (12%. El fototipo 3 (35% fue predominante; la exposición a las radiaciones ultravioletas fue moderada en 74%. Sólo 47% utilizaron protectores solares. Los anticuerpos anti-Ro se relacionaron en forma significativa con eritema malar, fotosensibilidad, livedo reticularis y alopecia. Los anticuerpos anti-Sm se relacionaron con fenómeno de Raynaud. Todas estas lesiones se observaron con mayor frecuencia en pacientes con enfermedad activa. La prevalencia de las manifestaciones mucocutáneas fue ligeramente más elevada a la informada en otras series. Hubo mayor prevalencia de eritema malar, alopecia difusa, fotosensibilidad y livedo reticularis en pacientes con enfermedad activa y anti-Ro (+ y de fenómeno Raynaud con anti-Sm.(+.The objective was to analyze the prevalence of mucocutaneous lesions in patients with systemic lupus erythematosus (SLE. During a 3-year period, we analyzed 77 patients with a diagnosis of SLE. The mucocutaneous lesions were classified into specific and non-specific. We defined skin type, sunlight exposure and photoprotection and correlated these lesions with serology and disease activity. Acute specific lesions were found in 67.5% of the patients, subacute lupus in 6.5% and chronic lesions in 26.0%. The most prevalent non-specific lesions were alopecia (59.7%, photosensitivity (57.1%, Raynaud

  13. Exposure to Indoor Allergens in Different Residential Settings and Its Influence on IgE Sensitization in a Geographically Confined Austrian Cohort.

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    Teresa Stemeseder

    Full Text Available Exposure to indoor allergens is crucial for IgE sensitization and development of allergic symptoms. Residential settings influence the allergen amount in house dust and hence allergic sensitization. Within this study, we investigated allergen exposure and molecule-based IgE levels in a geographically confined region and evaluated the impact of housing, pets and cleaning.501 adolescents from Salzburg, Austria participated in this cross-sectional study. House dust samples were examined regarding major mite, cat, dog, and mold allergens using a multiplex assay. Serum samples of participants were analyzed for specific IgE to Der p 1, Der p 2, Fel d 1, Can f 1 and Alt a 1 using the multiplex array ImmunoCAP ISAC. Information on allergies, living areas, dwelling form (house, flat, farm, pets, and household cleanliness were obtained by a questionnaire.In investigated house dust samples, the concentration of cat allergen was highest while the prevalence of mold allergens was very low. Participants showed IgE sensitization to Der p 1 (13.2%, Der p 2 (18.2%, Fel d 1 (14.4%, Can f 1 (2.4% and Alt a 1 (2.0%. In alpine regions, lower mite allergen concentrations were detected which correlated with reduced IgE levels. A trend for increased sensitization prevalence from rural to alpine to urban regions was noted. Living on farms resulted in lower sensitization prevalence to mite and cat allergens, even though exposure to mites was significantly elevated. The presence of cats was associated with a lower sensitization rate and IgE levels to cat and mite allergens, and less frequent allergic diseases. Cleaning did not impact allergen concentrations, while IgE reactivity to mites and allergic diseases were more pronounced when living in cleaner homes.Allergen exposure to indoor allergens was influenced by setting of homes. Living in a farm environment and having a cat at home showed a protective effect for IgE sensitization and allergies. This cross

  14. IgE antibodies to Hymenoptera venoms in the serum are common in the general population and are related to indications of atopy.

    Science.gov (United States)

    Schäfer, T; Przybilla, B

    1996-06-01

    Determination of Hymenoptera venom (HV)-specific serum IgE antibodies is a useful diagnostic method in patients with systemic anaphylactic reaction (SAR) to Hymenoptera stings. In a general population cohort, we determined the prevalence of SAR and HV-specific IgE antibodies and assessed parameters associated with the latter. A total of 277 voluntarily participating inhabitants of rural Bavaria (Germany) (232 adults, mean age 38.0 years; 45 children, mean age 8.4 years) were investigated for a history of atopic disease or SAR to insect stings; in 258 of these, total IgE and specific IgE antibodies to HV (Apis mellifera, Vespula vulgaris/germanica) and four common aeroallergens (birch pollen, grass pollen, house-dust mite, and cat dander) in the serum were determined. Nine (3.3%) subjects reported SAR to insect stings. In 27.1% of the sera, specific IgE antibodies to HV were found, to bee venom in 24.8%, and to wasp venom in 8.5% (P 100 kU/l was found in 22.5%. Specific serum IgE to HV was significantly associated with male sex (female vs. male, OR = 0.47; CI 0.25-0.86), young age (children vs. adults, OR = 2.80; CI 1.25-6.28), a history of SAR to insect stings (OR = 4.16; CI 1.15-15.03), total sIgE > 100 kU/l (OR = 3.88; CI 1.98-7.60), and specific IgE antibodies to three of the four aeroallergens (grass pollen, OR = 7.24 CI 3.66-14.38; birch pollen, OR = 3.67 CI 1.54-8.81; and house-dust mite, OR = 4.61 CI 2.08-10.32). It is concluded that immunologic sensitization to HV is common in the general population and is associated with atopy-related humoral IgE hyperresponsiveness.

  15. [Multiple myeloma (IgG-kappa) infiltrating central nervous system, lymph nodes, liver, and kidneys, and with elevation of IgE].

    Science.gov (United States)

    Toyota, Shigeo; Nakamura, Norihiko; Dan, Kazuo

    2004-05-01

    A 63-year-old man was admitted because of general malaise, fever, headache, generalized lymphadenopathy and hepatomegaly in July 2002. He was diagnosed as having multiple myeloma (MM) (IgG-kappa type) with atypical plasma cells in the bone marrow, lymph nodes and cerebrospinal fluid. Systemic and intrathecal chemotherapy were effective. Because of an increase of polyclonal IgE, electrophoretic patterns revealed an M-peak which was not as sharp as that in IgG myeloma. IgE production is not impaired by the pathologic process in MM patients.

  16. IgE versus IgG4 epitopes of the peanut allergen Ara h 1 in patients with severe allergy

    DEFF Research Database (Denmark)

    Bøgh, Katrine Lindholm; Nielsen, H.; Eiwegger, T.

    2014-01-01

    epitopes. Objective: The aim of this study was to compare Ara h 1-specific IgE and IgG4 epitope recognition patterns in patients with severe peanut allergy, applying a method allowing for identification of both linear and conformational epitopes. Methods: Polyclonal sera from three individual patients......, suffering from severe allergic reaction to peanuts, including anaphylaxis, were used to analyse the IgE and IgG4 epitope recognition patterns of the major peanut allergen Ara h 1. Epitope identification was conducted by competitive immuno-screening of a phage-displayed random heptamer peptide library...

  17. The Differences in Serum Quantitative Specific IgE Levels Induced by Dermatophagoides pteronyssinus, Dermatophagoides farinae and Blomia tropicalis Sensitization in Intermittent and Persistent Allergic Asthma

    Directory of Open Access Journals (Sweden)

    Agus Joko Susanto

    2018-01-01

    Full Text Available Background: house dust mites (HDM are an important inhalant allergen in allergic asthma. However, molecular diagnostic study of specific IgE to HDM allergens has not been done in Indonesia. In addition, the association of quantitative specific IgE measurement with asthma severity has not been investigatedd. This study aimed to investigate the difference of serum quantitative specific IgE levels induced by Dermatophagoides (D. pteronyssinus, D. farinae and Blomia tropicalis sensitization in intermittent and persistent allergic asthma. Methods: this was a cross-sectional study on adult allergic asthma patients who were invited for serum specific IgE testing. This study was a part of a larger study within the Division of Allergy and Immunology, Cipto Mangunkusumo Hospital. Asthma severity was defined based on Global Initiative on Asthma (GINA 2015 criteria and were grouped as intermittent or persistent. Quantitative specific IgE testing was done on blood serum using a multiple allergosorbent test (Polycheck Allergy, Biocheck GmbH, Munster, Germany. The HDM allergens tested were D. pteronyssinus, D. farinae, and Blomia tropicalis. Difference between two groups were analyze using Mann-Whitney test. Results: a total of 87 subjects were enrolled in this study; 69 (79.3% were women. Mean patients’ age was 40, 2 years. Sixty-three (72.4% subjects had asthma and allergic rhinitis. Fifty-eight (66.7% subjects were classified as persistent asthma. The prevalence of sensitization was 62.1% for D. farinae, 51.7% for D. pteronyssinus, and 48.3% for Blomia tropicalis. The median of specific IgE levels were significantly higher in persistent asthma compares to intermittent asthma induced by D. farinae (median 1.30 vs. 0.0 kU/L; p=0.024 and B. tropicalis (median 0.57 vs. 0.0 kU/L; p=0.015 sensitization. Level of Specific IgE  D. pteronyssinus was also to be higher in persistent asthma than the level measured in intermittent asthma (0.67 vs. 0.00 kU/L; p=0

  18. Analysis of calcium-induced conformational changes in calcium-binding allergens and quantitative determination of their IgE binding properties.

    Science.gov (United States)

    Parody, Nuria; Fuertes, Miguel Angel; Alonso, Carlos; Pico de Coaña, Yago

    2013-01-01

    The polcalcin family is one of the most epidemiologically relevant families of calcium-binding allergens. Polcalcins are potent plant allergens that contain one or several EF-hand motifs and their allergenicity is primarily associated with the Ca(2+)-bound form of the protein. Conformation, stability, as well as IgE recognition of calcium-binding allergens greatly depend on the presence of protein-bound calcium ions. We describe a protocol that uses three techniques (SDS-PAGE, circular dichroism spectroscopy, and ELISA) to describe the effects that calcium has on the structural changes in an allergen and its IgE binding properties.

  19. Molecular cloning, expression and immunological characterisation of Lol p 5C, a novel allergen isoform of rye grass pollen demonstrating high IgE reactivity.

    Science.gov (United States)

    Suphioglu, C; Mawdsley, D; Schäppi, G; Gruehn, S; de Leon, M; Rolland, J M; O'Hehir, R E

    1999-12-03

    A novel isoform of a major rye grass pollen allergen Lol p 5 was isolated from a cDNA expression library. The new isoform, Lol p 5C, shares 95% amino acid sequence identity with Lol p 5A. Both isoforms demonstrated shared antigenic activity but different allergenic activities. Recombinant Lol p 5C demonstrated 100% IgE reactivity in 22 rye grass pollen sensitive patients. In comparison, recombinant Lol p 5A showed IgE reactivity in less than 64% of the patients. Therefore, Lol p 5C represents a novel and highly IgE-reactive isoform allergen of rye grass pollen.

  20. Edema de disco óptico en glaucoma primario por cierre angular agudo: diagnóstico diferencial con edema de disco en neuropatía óptica isquémica anterior no arterítica y arterítica

    Directory of Open Access Journals (Sweden)

    Andrea Díaz Barrón

    2016-05-01

    Discusión: La coexistencia de edema de papila y PIO elevada puede deberse, bien a la congestión del nervio óptico secundaria a un GPCAA, bien a una NOIA concomitante, por lo que es de gran relevancia hacer un correcto diagnóstico diferencial en relación con su evolución clínica y morfológica.

  1. autorregulado con estudiantes universitarios

    Directory of Open Access Journals (Sweden)

    Jairo Andrés Montes

    2005-01-01

    Full Text Available El propósito del presente estudio es describir la forma en la que se presentan los procesos de aprendizaje autorregulado con un grupo de estudiantes (22 estudiantes de tercer semestre de Psicología de la PUJ, Cali, en el evento de preparación para la presentación un examen. Asimismo se describen las correlaciones que ocurren entre las distintas fases de dicho proceso de autorregulación del aprendizaje. Para conseguir los objetivos propuestos se ha hecho uso de una observación de desempeño en tiempo real, es decir, de la observación durante una sesión de preparación de examen de los estudiantes, en la cual se emplearon protocolos verbales para dar cuenta de lo que «pasaba por su mente» mientras estudiaban. Una entrevista semi-estructurada y una prueba objetiva. Los resultados fueron analizados a la luz del modelo mixto de procesamiento de información y constructivismo abordado por Winne(1998. Como resultado se encontró una relación significativa entre los niveles de desempeño en el proceso de ARR y el resultado del examen. Igualmente se encontraron bajos niveles de regulación en una parte importante de la muestra y un desfase significativo entre conocimiento declarativo de ARR y desempeño en el mismo

  2. Algunas variables bioquímicas en adolescentes obesos con antecedente de bajo peso al nacer

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    María del Carmen Valdés Alonso

    Full Text Available Introducción: la malnutrición intraútero ha sido postulada con vinculación a afecciones del desarrollo embrionario que determinan el desarrollo ulterior de obesidad, diabetes mellitus tipo 2, dislipidemias, disfunción mitocondrial y aterosclerosis. Objetivo: determinar las complicaciones metabólicas en adolescentes obesos con antecedentes de bajo peso al nacer. Métodos: estudio descriptivo y de corte transversal. Se incluyeron 50 pacientes obesos, con antecedentes de bajo peso al nacer y embarazo a término, de ambos sexos, en las edades comprendidas entre 9 y 17 años de edad, que asistieron a la consulta externa del servicio de Endocrinología del Hospital Pediátrico Docente "Juan Manuel Márquez", en el período comprendido de enero de 2011 a enero de 2012. Se realizaron determinaciones de colesterol, triglicéridos, aminotransferasas hepáticas, glucemia, e insulinemia en ayunas y posprandial de dos horas. Resultados: las variables bioquímicas estudiadas no mostraron diferencias entre los puntos de corte utilizados para clasificar el peso al nacer. La transaminasa glutámico pirúvica mostró valores más altos en los adolescentes entre 15 y 17 años de edad. Conclusiones: la hipertrigliceridemia fue más elevada en el sexo femenino, mientras que la hiperglucemia y la hiperinsulinemia lo fueron en el masculino.

  3. Suspensión retropúbica en mujeres con incontinencia urinaria de esfuerzo

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    Amparo Mirabal Fariñas

    2014-09-01

    Full Text Available Se efectuó un estudio descriptivo y transversal de 60 pacientes con incontinencia urinaria de esfuerzo, atendidas en la consulta de Urología del Hospital Provincial Docente Clinicoquirúrgico "Saturnino Lora Torres" de Santiago de Cuba, de enero del 2003 a igual mes del 2012, con vistas a evaluar la aplicación de la técnica quirúrgica de suspensión retropúbica en ellas. Entre los resultados relevantes de la serie figuraron: edad promedio de 50 años, incontinencia urinaria de esfuerzo de grados III (48,0 % y II (28,0 % y un elevado porcentaje de continencia posoperatoria inmediata (95,3. A los 3 meses de practicada la intervención, la continencia fue de 92,2 %, mientras que luego de 6 y 9 meses, se obtuvo una continencia de 87,5 %. Se concluyó que la suspensión retropúbica es un procedimiento de elevada efectividad para tratar pacientes con incontinencia urinaria de esfuerzo e incontinencia urinaria mixta, debido a su simple realización y las escasas complicaciones que se producen, lo cual incide favorablemente en la estadía hospitalaria

  4. Hiperinmunoglobulinemia E: una inmunodeficiencia primaria con compromiso de varios mecanismos de la respuesta inmune

    Directory of Open Access Journals (Sweden)

    Juan E. Pérez

    1998-02-01

    Full Text Available El síndrome de hiperinmunoglobulinemia E(SHIE es una Inmunodeficiencia Primaria (IOP de origen desconocido, cuya fisiopatología no ha sido -esclarecida. Sus manifestaciones comienzan en las primeras semanas de vida. Es característico en su expresión clínica el desarrollo de un síndrome de infección recurrente patológica, con neumopatía supurativa y neumatoceles perennes principalmente producidos porStaphylococcusaureus; se encuentran niveles séricos muy elevados de IgE. En nuestras investigaciones sobre la patogénesis del ?HIE, la hipótesis propone varias alteraciones como causas importantes para su presentación: Trastornos en la activación y generación de memoria de los linfocitos T, posiblemente debida a una expresión e interacción inadecuadas de las moléculas de superficie involucradas en este fenómeno (CO40L, CO80, CO86, CTLA-4; un patrón de citoquinas diferente, con producción incrementada de IL-13 que sería la responsable del exceso de síntesis de la IgE y del factor estimulante de colonias de granulocitos y monolitos G M-CSF (G M-CS F= Granulocyte-Monocyte Colony Stimulating Factor el cual modularía la alteración en la función de las células fagocíticas.

  5. Core alpha1-->3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep

    NARCIS (Netherlands)

    van Die, I.; Gomord, V.; Kooyman, F. N.; van den Berg, T. K.; Cummings, R. D.; Vervelde, L.

    1999-01-01

    Synthesis of parasite specific IgE plays a critical role in the defence against helminth infections. We report here that IgE from serum from Schistosoma mansoni infected mice and Haemonchus contortus infected sheep recognizes complex-type N-glycans from Arabidopsis thaliana, which contain

  6. Dimensiones de personalidad, sentido de coherencia y salud percibida en pacientes con un síndrome fibromiálgico

    Directory of Open Access Journals (Sweden)

    José Besteiro

    2008-01-01

    Full Text Available Se evaluaron los rasgos de personalidad, el sentido de coherencia y la salud percibida en pacientes diagnosticadas con un síndrome fibromiálgico. Se utilizaron como controles pacientes diagnosticadas con artritis reumatoide y sujetos sanos. Se realizó un estudio ex post facto de tipo prospectivo. Tanto a las pacientes fibromiálgicas como a los controles se les aplicó el cuestionario de personalidad NEO PI-R, el OLQ de Antonovsky para evaluar el Sentido de Coherencia y el Cuestionario de Salud SF- 36 para evaluar la salud percibida. Las pacientes con fibromialgia obtuvieron puntuaciones más elevadas en neuroticismo y un sentido de coherencia más débil con niveles inferiores en las dimensiones de comprensibilidad y significado que las pacientes con artritis reumatoide y los controles sanos. Respecto a los índices de calidad de vida y a la salud percibida, las pacientes con fibromialgia muestran alteraciones en todas las dimensiones respecto a los grupos control. Los resultados obtenidos indican que las pacientes con fibromialgia presentan rasgos de personalidad y creencias de control que podrían actuar de forma patogénica favoreciendo la aparición de la enfermedad y alterando la percepción subjetiva de la misma.

  7. Puentes con vigas pretensadas

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1965-07-01

    Full Text Available This paper describes one of the three bridges which Hidrocivil, S. A., has built in Catalonia (northern Spain, over the river Ripoll. The other two bridges are very similar to this one, both in construction and design, and show only minor adjustments to the local topography. The contracting firm proposed several alterations in the prefabrication and constructional procedure, in relation to the initial project, and these changes were accepted. The main feature of these projects is the use of prestressed beams, built at the workshop in sections, and joined together by means of sixty 7 mm cables in each beam. As the shear forces are more acute at the joints, the end of each section has a kind of diaphragm, to provide a large contact area, and hence greater surface to transmit the shear forces. The methods of construction are also of interest. Briefly, they involve building the bridge piles, and use these to support a provisional structure with transversal movement. This provisional structure, in turn, served as platform for two bridge cranes, which lifted the girders to their final location. After the first span was completed, the deck was concreted and the auxiliary structure pushed forward to the next span, to repeat the same operations. This arrangement saved the use of provisional framework.En este trabajo se describe uno de los tres puentes que Hidrocivil, S. A., ha construido.—previo concurso— en la región catalana; concretamente, el que salva el río Ripoll. Los otros dos no han sido objeto de descripción general por ser muy similares, en lo que a ejecución y concepción se refiere, con la única variante que presentan las características topográficas locales. La empresa propuso ciertas variantes— que fueron aceptadas— en la prefabricación y métodos de construcción. El interés de estas obras se centra en el empleo de vigas pretensadas, prefabricadas en taller por trozos, y solidarizados en el mismo mediante las operaciones

  8. Violencia con el anciano

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    Rita Campillo Motilva

    2002-08-01

    Full Text Available La violencia doméstica es tan antigua como la humanidad misma y se reconocen la violencia infantil, contra la mujer y al anciano, fundamentalmente; siendo este último grupo una población en ascenso por las mayores expectativas de vida de los últimos años. Como resultado de ello, el número de casos de abuso en el anciano se incrementará y el impacto de este abuso sobre la salud debe ser considerado de forma adecuada. La gama de maltratos es variadísima e incluye el abuso físico, emocional, financiero, sexual, por negligencia, negación a brindarle ayuda y otras formas más. Los ancianos con deterioro cognitivo son los más vulnerables. El médico en la atención primaria de salud es un pilar importante en la prevención y educación de este problema.Domestic violence is as old as humanity itself. Child, women and elderly abuse are mainly recognized. The elderly group is increasing due to the higher life expectancy experimented during the last years. As a result, the number of battered elderly will grow and the impact of this abuse on health should be adequately considered. The range of abuse is very wide and it includes physical, emotional, financial and sexual abuse, negligence, rejection to give assistance and others. The elderly with cognitive deterioration are the most vulnerable. The physician at the primary health care level is an important milestone in the prevention and education of this problem.

  9. The usefulness of casein-specific IgE and IgG4 antibodies in cow's milk allergic children

    Directory of Open Access Journals (Sweden)

    Ito Komei

    2012-01-01

    Full Text Available Abstract Background Cow's milk allergy is one of the most common food allergies among younger children. We investigated IgE antibodies to milk, and IgE and IgG4 antibodies to casein, α-lactalbumin and β-lactoglobulin in cow's milk allergic (CMA and non-allergic (non-CMA children in order to study their clinical usefulness. Methods Eighty-three children with suspected milk allergy (median age: 3.5 years, range: 0.8-15.8 years were diagnosed as CMA (n = 61 or non-CMA (n = 22 based on an open milk challenge or convincing clinical history. Their serum concentrations of allergen-specific (s IgE and IgG4 antibodies were measured using ImmunoCAP®. For the sIgG4 analysis, 28 atopic and 31 non-atopic control children were additionally included (all non-milk sensitized. Results The CMA group had significantly higher levels of milk-, casein- and β-lactoglobulin-sIgE antibodies as compared to the non-CMA group. The casein test showed the best discriminating performance with a clinical decision point of 6.6 kUA/L corresponding to 100% specificity. All but one of the CMA children aged > 5 years had casein-sIgE levels > 6.6 kUA/L. The non-CMA group had significantly higher sIgG4 levels against all three milk allergens compared to the CMA group. This was most pronounced for casein-sIgG4 in non-CMA children without history of previous milk allergy. These children had significantly higher casein-sIgG4 levels compared to any other group, including the non-milk sensitized control children. Conclusions High levels of casein-sIgE antibodies are strongly associated with milk allergy in children and might be associated with prolonged allergy. Elevated casein-sIgG4 levels in milk-sensitized individuals on normal diet indicate a modified Th2 response. However, the protective role of IgG4 antibodies in milk allergy is unclear.

  10. Receptividade do estigma e desenvolvimento do tubo polínico em flores de pessegueiro submetidas à temperatura elevada Stigma receptivity and pollen tube development in peach flowers submitted to high temperature

    Directory of Open Access Journals (Sweden)

    Ilisandra Zanandrea

    2011-12-01

    Full Text Available Dentre os diversos fatores que afetam a produtividade do pessegueiro em regiões subtropicais, está a ocorrência de temperaturas elevadas no início da floração. Tais temperaturas podem causar danos ao estigma e à germinação do grão de pólen, ocasionando decréscimo na fecundação e na fixação dos frutos. O presente trabalho teve por objetivo detectar diferenças entre genótipos quanto à tolerância à temperatura elevada (29±2ºC, bem como verificar se tais diferenças poderiam ser detectadas por um processo simples, utilizando ramos destacados. Para isso, foram realizados dois experimentos, sendo um com ramos destacados e outro com plantas inteiras em vasos, obtidas por enxertia, no outono. As estimativas de graus de receptividade do estigma e de comprimento do tubo polínico nos ramos destacados sugerem que as seleções Conserva 1566 e Conserva 693 e a cv. 'Maciel' não sofrem negativamente influência da temperatura de 29°C. A receptividade do estigma, mesmo em ramos destacados, pode discriminar os genótipos quanto à tolerância da parte feminina a temperaturas próximas a 29°C.Several factors can cause an erratic production of peaches under subtropical conditions. The occurrence of high temperatures on the beginning of blooming is one of them. Such temperatures can damage the stigma and the pollen germination, causing a decrease on fecundation and fruit set. The present work had the objective of looking for differences in tolerance to 29±2ºC among peach genotypes, as well as checking if a simple method using detached twigs would be suitable to detect differences. Two experiments were conducted using whole plants in vase, on one and detached twigs on the other. Estimates of pollen tube growth on the pistil and stigma receptivity suggested that selections Conserva 1566 and Conserva 693 and cv. 'Maciel' were tolerant to temperatures around 29°C at begining of blooming. Stigma receptivity, even in detached twigs, showed

  11. Morfometria da mucosa duodenal em frangos de corte submetidos à temperatura ambiente cíclica elevada Intestinal morphometry of the duodenal mucosa in broiler chickens underwent to high cyclic environment temperature

    Directory of Open Access Journals (Sweden)

    C.F.P. Marchini

    2009-04-01

    Full Text Available Os efeitos da temperatura ambiente cíclica elevada sobre a morfometria da mucosa duodenal e o peso corporal em frangos de corte foram avaliados. Setenta pintos de corte, machos, foram alojados em gaiolas e distribuídos em dois grupos. Um grupo foi submetido diariamente, durante uma hora, à temperatura ambiente cíclica elevada do primeiro até o 42º dia de idade (ambiente ST; e outro foi mantido em conforto térmico (ambiente TN. Cinco frangos de cada grupo foram sacrificados, semanalmente, por deslocamento cervical para mensuração da altura de vilosidades (VI, profundidade das criptas (CR e relação vilo/cripta (VI/CR duodenal. Dez aves de cada grupo foram pesadas semanalmente em balança digital. Utilizou-se delineamento inteiramente ao acaso em esquema fatorial 7x2 (sete idades: um, sete, 14, 21, 28, 35 e 42 dias, e dois ambientes: ST e TN. Os ambientes foram comparados pelo teste de Fisher (PThe effects of high cyclic environment temperature on body weight and morphometry of the duodenal mucosa in broiler chicken were evaluated. Seventy one-day-old male broiler chicks were sheltered in cages and distributed in two groups. One group was daily exposed to high cyclic environment temperature for an hour, from hatching to 42 days of age (group ST, the other one was kept under thermoneutral conditions (group TN. Five chickens of each group were weekly slaughtered by cervical delocation to mesure the villosities height (VI, crypts depth (CR, and villo/crypt ratio (VI/ CR in the duodenum. Ten chickens of each group were weighted weekly on a digital balance. A completely randomized experimental design in a 7x2 factorial arrangement (hatching, seven, 14, 21, 28, 35, and 42 days of age and two environments: ST and TN. The environments were compared by Fisher test (P<0.05 and the effects of days of life by polynomial regression. The ST group had reduction in VI at 14 and 21 days of age (P<0.01, CR at 28 days of age (P<0.05, and in VI/CR at 21

  12. The Importance of Dosimetry to the Medical Management of Persons Accidentally Exposed to High Levels of Radiation; Le Role de la Dosimetrie Dans les Soins Medicaux a Donner aux Personnes Accidentellement Exposees a de Fortes Doses de Rayonnement; 0417 041d 0410 0427 0414 ; Importancia de la Dosimetria para el Tratamiento Medico de las Personas Accidentalmente Expuestas a Dosis Elevadas de Radiaciones

    Energy Technology Data Exchange (ETDEWEB)

    Andrews, G. A.; Lushbaugh, C. C. [Medical Division, Oak Ridge Institute of Nuclear Studies (United States); Auxier, J. A. [Health Physics Division, Oak Ridge National Laboratory, Oak Ridge, TN (United States)

    1965-06-15

    Radiation accidents continue to occur sporadically and it appears that there is no hope of eliminating them entirely. The management of patients exposed to large doses of radiation is constantly improving because of increasing ability to prevent and treat infection and to control haemorrhage. The possible effectiveness of temporary bone marrow grafts offers promise of a further reduction in mortality. The use of this growing biological information is closely related to, and to a large extent dependent upon, improvements in physical dosimetry for radiation accidents. (author) [French] Des accidents provoques par les rayonnements continuent a se produire de temps a autre et il ne semble guere que l'on puisse esperer les eliminer entierement. Toutefois, le traitement des sujets exposes a de fortes doses de rayonnement ne cesse de s'ameliorer; on parvient, en effet, dans une mesure croissante a empecher et a traiter l*infection ainsi qu'a arreter l'hemorragie. Comme la greffe temporaire de la moelle des os semble devoir donner des resultats, on peut s*attendre a une nouvelle reduction du taux de mortalite. L'application de ces connaissances biologiques sans cesse accrues est fonction, dans une large mesure, des ameliorations apportees a la dosimetrie physique des accidents nucleaires. (author) [Spanish] De vez en cuando se siguen produciendo accidentes de irradiacion y las perspectivas de evitarlos por completo parecen ser bastante remotas. El tratamiento de las victimas expuestas a dosis elevadas de radiaciones mejora constantemente debido a la creciente posibilidad de prevenir y tratar las infecciones, y de controlar las hemorragias. Tambien es posible que la eficacia de ios injertos temporales de medula osea se traduzca en una disminucion de la mortalidad. El aprovechamiento de estos conocimientos biologicos cada vez mas amplios guarda una estrecha relacion con los perfeccionamientos en materia de dosimetria fisica en caso de accidentes de irradiacion. (author

  13. Specific IgE antibodies to vespids in the course of immunotherapy with Vespula germanica administered to patients sensitized to Polistes dominulus.

    Science.gov (United States)

    Juarez, C; Blanca, M; Miranda, A; Sanchez, F; Carmona, M J; Avila, M J; Fernandez, S; Fernandez, J; Terrados, S

    1992-08-01

    Sera from a group of 12 patients with anaphylactic reactions to vespids were studied. Field observations and RAST values suggested that the offending insect was Polistes dominulus (PD). Specific IgE antibodies to PD appeared in all cases and to Vespula germanica (VG) in nine. Absorption studies in these basal sera showed that IgE antibodies to VG were due to cross-reactivity with PD. The RAST value to both venoms was higher after immunotherapy (IT) in six cases. IgE antibodies increased to determinants common to both vespids, and in 41% of the cases to specific epitopes of VG venom allergens not initially detected in the basal sera. In one case antibodies increased only to VG without a corresponding rise to PD. These results indicate that if the correct venom to which the individuals are sensitized is not administered IgE antibodies may appear which were not initially detected in the patients' sera. The levels of these antibodies declined during the course of IT.

  14. IgE vs IgG4 epitopes of the peanut allergen Ara h 1 in patients with severe allergy

    DEFF Research Database (Denmark)

    Bøgh, Katrine Lindholm; Nielsen, H.; Eiwegger, T.

    2013-01-01

    to the allergen. However, recent studies have demonstrated the very importance of the IgG4-epitope affinity for the blocking ability. Studies comparing IgE and IgG4 binding epitopes mainly focus on the identification of linear epitopes. Peanut allergy is one of the most severe and persistent forms of food allergy....... The importance of conformational epitopes, of the major peanut allergen Ara h 1, has been demonstrated. The aim of this study was to compare Ara h 1-specific epitope patterns for IgE and IgG4 in patients with severe peanut allergy applying a method suitable to identify both linear and conformational epitopes....... Methods: Ara h 1-specific IgE and IgG4 epitope patterns were examined by competitive immunoscreening of a phage-displayed random 7-mer peptide library using polyclonal IgE and IgG4 from three individual patients suffering from severe peanut allergy. The resulting peptide sequences were mapped...

  15. IgE production by normal human lymphocytes is induced by interleukin 4 and suppressed by interferons gamma and alpha and prostaglandin E2

    NARCIS (Netherlands)

    Pène, J.; Rousset, F.; Brière, F.; Chrétien, I.; Bonnefoy, J. Y.; Spits, H.; Yokota, T.; Arai, N.; Arai, K.; Banchereau, J.

    1988-01-01

    The effect of human recombinant interleukin 4 (IL-4) on antibody production by normal peripheral blood mononuclear cells enriched for B cells was investigated. IL-4 preferentially induced IgE synthesis in vitro. In addition, a low induction of IgG production was observed, whereas IL-4 had no effect

  16. The Relationship Between Serum Levels of Total IgE, IL-18, IL-12, IFN- γ and Disease Severity in Children With Atopic Dermatitis

    Directory of Open Access Journals (Sweden)

    2006-01-01

    Full Text Available Studies about the role of cytokines on the immunopathogenesis of atopic dermatitis (AD are generally based on in vitro observations and this role has not been completely clarified yet. Serum levels of total IgE, IL-18, IL-12, IFN- γ and the relationship between these parameters and disease severity, determined using the SCORAD index, in a group of atopic patients were investigated in this study. Serum levels of total IgE were measured by the nephelometric method and serum levels of IL-18, IL-12/p40 and IFN- γ were measured by ELISA method. Serum levels of total IgE and IL-18 were found significantly higher in study group than in controls ( p<.001 . There was no statistically significant difference between patients and controls in respect of serum levels of IL-12/p40 ( p=.227 . A statistically significant relationship between SCORAD values and serum levels of total IgE ( p<.001 , IL-18 ( p<.001 , and IL-12/p40 ( p<.001 was determined. These results show that serum levels of IL-18 can be a sensitive parameter that importantly correlates with clinical severity of AD, can play a role in the immunopathogenesis of AD, and furthermore may be used in the diagnosis and follow-up of the disease in addition to other parameters.

  17. Elevated levels of IgG and IgG4 to Malassezia allergens in atopic eczema patients with IgE reactivity to Malassezia

    NARCIS (Netherlands)

    Johansson, Catharina; Tengvall Linder, Maria; Aalberse, Rob C.; Scheynius, Annika

    2004-01-01

    The opportunistic yeast Malassezia is considered to be one of the factors that can contribute to atopic eczema (AE). Elevated serum IgE levels, T-cell proliferation and positive skin prick test (SPT) and atopy patch test (APT) reactions to Malassezia are found among AE patients. Sera from 127 AE

  18. Evaluation of the Role of IgE Responses to Der p 1 and Der p 2 in Chinese House Dust Mite-Allergic Patients

    NARCIS (Netherlands)

    Wang, Hui-Ying; Gao, Zhong-Shan; Zhou, Xiang; Dai, Yu; Yao, Wo; Zhang, Xiao-Feng; Yang, Zhao-Wei; Wu, Shan-Dong; Yu, Chao-Hui; Yang, Xu-Yan; van Ree, Ronald

    2015-01-01

    The role of specific IgE (sIgE) against Der p 1 and Der p 2 in Chinese patients with house dust mite (HDM) allergy has not yet been well investigated. One hundred patients were enrolled, based on sensitization and doctor-diagnosed allergy to HDM. Questionnaires were administered to document

  19. Are allergic multimorbidities and IgE polysensitization associated with the persistence or re-occurrence of foetal type 2 signalling? The MeDALL hypothesis

    NARCIS (Netherlands)

    Bousquet, J.; Anto, J. M.; Wickman, M.; Keil, T.; Valenta, R.; Haahtela, T.; Carlsen, K. Lodrup; van Hage, M.; Akdis, C.; Bachert, C.; Akdis, M.; Auffray, C.; Annesi-Maesano, I.; Bindslev-Jensen, C.; Cambon-Thomsen, A.; Carlsen, K. H.; Chatzi, L.; Forastiere, F.; Garcia-Aymerich, J.; Gehrig, U.; Guerra, S.; Heinrich, J.; Koppelman, G. H.; Kowalski, M. L.; Lambrecht, B.; Lupinek, C.; Maier, D.; Melen, E.; Momas, I.; Palkonen, S.; Pinart, M.; Postma, D.; Siroux, V.; Smit, H. A.; Sunyer, J.; Wright, J.; Zuberbier, T.; Arshad, S. H.; Nadif, R.; Thijs, C.; Andersson, N.; Asarnoj, A.; Ballardini, N.; Ballereau, S.; Bedbrook, A.; Benet, M.; Bergstrom, A.; Brunekreef, B.; Burte, E.; Calderon, M.; De Carlo, G.; Demoly, P.; Eller, E.; Fantini, M. P.; Hammad, H.; Hohman, C.; Just, J.; Kerkhof, M.; Kogevinas, M.; Kull, I.; Lau, S.; Lemonnier, N.; Mommers, M.; Nawijn, M.; Neubauer, A.; Oddie, S.; Pellet, J.; Pin, I.; Porta, D.; Saes, Y.; Skrindo, I.; Tischer, C. G.; Torrent, M.; von Hertzen, L.

    Allergic diseases [asthma, rhinitis and atopic dermatitis (AD)] are complex. They are associated with allergen-specific IgE and nonallergic mechanisms that may coexist in the same patient. In addition, these diseases tend to cluster and patients present concomitant or consecutive diseases

  20. Isolation of high-affinity human IgE and IgG antibodies recognising Bet v 1 and Humicola lanuginosa lipase from combinatorial phage libraries

    DEFF Research Database (Denmark)

    Jakobsen, Charlotte G; Bødtger, Uffe; Kristensen, Peter

    2004-01-01

    Allergen-specific Fab fragments isolated from combinatorial IgE and IgG libraries are useful tools for studying allergen-antibody interactions. To characterise the interaction between different allergens and antibodies we have created recombinant human phage antibody libraries in the Fab format...

  1. Specific IgE and IgG4 immune responses to tetanus and diphtheria toxoid in atopic and nonatopic children during the first two years of life

    NARCIS (Netherlands)

    Dannemann, A.; van Ree, R.; Kulig, M.; Bergmann, R. L.; Bauer, P.; Forster, J.; Guggenmoos-Holzmann, I.; Aalberse, R. C.; Wahn, U.

    1996-01-01

    BACKGROUND: In order to investigate, whether atopic and nonatopic children show differences in their specific IgE and IgG4 immune responses to tetanus (T) and diphtheria (D) antigens, we studied 538 children who had been followed from birth on and from whom records had been kept of all

  2. Comparison of an enzyme linked immunosorbent assay (ELISA) and a radioallergosorbent test (RAST) for detection of IgE antibodies to Brugia malayi

    NARCIS (Netherlands)

    Wahyuni, Sitti; van Ree, Ronald; Mangali, Andarias; Supali, Taniawati; Yazdanbakhsh, Maria; Sartono, Erliyani

    2003-01-01

    The enzyme linked immunosorbent assay (ELISA) for specific IgE antibodies to Brugia malayi was compared with the radioallergosorbent test (RAST) for use in immunoepidemiological studies of lymphatic filariasis. Sera used were from individuals (aged 5-82 years) living in an area endemic for lymphatic

  3. Relation between IgG antibodies to foods and IgE antibodies to milk, egg, cat, dog and/or mite in a cross-sectional study

    NARCIS (Netherlands)

    Eysink, P. E.; de Jong, M. H.; Bindels, P. J.; Scharp-van der Linden, V. T.; de Groot, C. J.; Stapel, S. O.; Aalberse, R. C.

    1999-01-01

    Because IgG antibodies to foods can be detected before IgE antibodies to inhalants, increased levels of IgG antibodies to foods might be used as a predictor of IgE-mediated allergy in initially nonatopic children. To examine the cross-sectional relation between IgG to foods (i.e. mixture of wheat

  4. Rapidly boosted Plasma IL-5 induced by treatment of human Schistosomiasis haematobium is dependent on antigen dose, IgE and eosinophils

    DEFF Research Database (Denmark)

    Wilson, Shona; Jones, Frances M.; Fofana, Hassan K. M.

    2013-01-01

    IgE specific to worm antigen (SWA) and pre-treatment eosinophil number, are associated with human immunity to re-infection with schistosomes after chemotherapeutic treatment. Treatment significantly elevates circulating IL-5 24-hr post-treatment of Schistosoma mansoni. Here we investigate...

  5. The effect of three-monthly albendazole treatment on Th2 responses: Differential effects on IgE and IL-5.

    Science.gov (United States)

    de Ruiter, K; Tahapary, D L; Wammes, L J; Wiria, A E; Hamid, F; van Lieshout, L; Smit, J W A; Houwing-Duistermaat, J J; Sartono, E; Supali, T; Yazdanbakhsh, M

    2017-06-01

    Helminth parasites induce a strong Th2 response, characterized by high levels of IgE and elevated signature cytokines such as IL-5. As many global deworming programmes are underway, there is concern that this might lead to emergence of Th1-mediated pathologies when the counterbalancing helminth-induced Th2 response is absent. Therefore, we assessed the effect of deworming on Th2-mediated responses in a household-clustered randomized controlled trial in Indonesia. Total plasma IgE and whole-blood IL-5 responses to mitogen phytohaemagglutinin (PHA) were measured in 1494 and 682 subjects, respectively, at baseline, 9 and 21 months after three-monthly single-dose treatment with albendazole or placebo. Anthelmintic treatment did not result in complete removal of helminth infections in the community. However, treatment significantly decreased IgE levels in albendazole- compared to placebo-treated subjects. IL-5 responses to PHA were not significantly affected by anthelmintic treatment and tended to increase in albendazole-treated subjects, indicating that intensive treatment of helminth parasites has different outcomes on B-cell (IgE levels) and T-cell (IL-5) responses. The data shows that 2 years of deworming can have differential effects on responses typified as Th2-mediated, which needs to be taken into account when examining the impact of helminths on noncommunicable diseases. © 2017 John Wiley & Sons Ltd.

  6. The effect of three-monthly albendazole treatment on Th2 responses: Differential effects on IgE and IL-5

    NARCIS (Netherlands)

    Ruiter, K. de; Tahapary, D.L.; Wammes, L.J.; Wiria, A.E.; Hamid, F.; Lieshout, L. van; Smit, J.W.A.; Houwing-Duistermaat, J.J.; Sartono, E.; Supali, T.; Yazdanbakhsh, M.

    2017-01-01

    Helminth parasites induce a strong Th2 response, characterized by high levels of IgE and elevated signature cytokines such as IL-5. As many global deworming programmes are underway, there is concern that this might lead to emergence of Th1-mediated pathologies when the counterbalancing

  7. GENERALIZED ANALYSIS OF MONOCLONAL ANTIBODIES TO IGE IN THE TREATMENT OF BRONCHIAL ASTHMA IN CHILDREN IN RUSSIAN FEDERATION

    Directory of Open Access Journals (Sweden)

    T.V. Kulichenko

    2011-01-01

    Full Text Available The article presents a generalized 2-year Russian experience in the application of monoclonal antibodies to IgE in children and adolescents with severe, resistant to standard treatment of bronchial asthma. Data on 65 patients treated with anti-IgE therapy with omalizumab within more than 6 months period in 6 centers of the Russian Federation from 2007 to 2010 were analyzed. The results of this analysis demonstrate the safety and high clinical effectiveness of anti-IgE-therapy in children and adolescents with severe uncontrolled atopic bronchial asthma. Full control of the disease or marked improvement can be achieved in 73% patients, the effect in most cases significantly increases during the first 6 months of treatment and becomes stable in the future, providing a good quality of life and normal lung function.Key words: bronchial asthma, anti-IgE-therapy, treatment, adolescents, children, quality of life, omalizumab.

  8. Testing the "toxin hypothesis of allergy": Mast cells, IgE, and innate and acquired immune responses to venoms*

    Science.gov (United States)

    Tsai, Mindy; Starkl, Philipp; Marichal, Thomas; Galli, Stephen J.

    2015-01-01

    Summary Work in mice indicates that innate functions of mast cells, particularly degradation of venom toxins by mast cell-derived proteases, can enhance resistance to certain arthropod or reptile venoms. Recent reports indicate that acquired Th2 immune responses associated with the production of IgE antibodies, induced by Russell’s viper venom or honeybee venom, or by a component of honeybee venom, bee venom phospholipase 2 (bvPLA2), can increase the resistance of mice to challenge with potentially lethal doses of either of the venoms or bvPLA2. These findings support the conclusion that, in contrast to the detrimental effects associated with allergic Th2 immune responses, mast cells and IgE-dependent immune responses to venoms can contribute to innate and adaptive resistance to venom-induced pathology and mortality. PMID:26210895

  9. IgE antipolymyxin B antibody formation in a T cell-depleted bone marrow transplant patient

    International Nuclear Information System (INIS)

    Lakin, J.D.; Grace, W.R.; Sell, K.W.

    1975-01-01

    The production of IgE-class antibody specific for polymyxin B is documented in an 18-year-old white female acute myelocytic leukemic patient in relapse. The patient was rendered T cell--deficient by total-body x irradiation and antihuman thymocyte globulin for the purpose of bone marrow transplantation. Thereafter, symptoms of nasal congestion, rhinorrhea, and perinasal urtication produced by topical application of a polymyxin solution were noted. Reaginic activity mediated by an IgE antibody against polymyxin is documented by Prausnitz-Kuestner--type passive transfer reactions and by an indirect hemagglutination technique developed for these studies. The occurrence of type I hypersensitivity to this topical antibiotic is rare. It is speculated that pharmaceuticals normally having a low sensitizing potential might demonstrate increased reaginic immunogenicity in a spontaneously or iatrogenically T cell-depleted patient

  10. Specific IgE to fish extracts does not predict allergy to specific species within an adult fish allergic population.

    Science.gov (United States)

    Schulkes, Karlijn Jg; Klemans, Rob Jb; Knigge, Lidy; de Bruin-Weller, Marjolein; Bruijnzeel-Koomen, Carla Afm; Marknell deWitt, Asa; Lidholm, Jonas; Knulst, André C

    2014-01-01

    Fish is an important cause of food allergy. Studies on fish allergy are scarce and in most cases limited to serological evaluation. Our objective was to study patterns of self-reported allergy and tolerance to different commonly consumed fish species and its correlation to IgE sensitization to the same species. Thirty-eight adult fish allergic patients completed a questionnaire regarding atopy, age of onset and symptoms to 13 commonly consumed fish species in the Netherlands (pangasius, cod, herring, eel, hake, pollock, mackerel, tilapia, salmon, sardine, tuna, plaice and swordfish). Specific IgE to these fish extracts were analyzed by ImmunoCAP. Median age of onset of fish allergy was 8.5 years. Severe reactions were reported by the majority of patients (n = 20 (53%) respiratory and of these 20 patients, 6 also had cardiovascular symptoms). After diagnosis, 66% of the patients had eliminated all fish from their diet. Allergy to all species ever tried was reported by 59%. In relation to species ever tried, cod (84%) and herring (79%) were the most frequently reported culprit species while hake (57%) and swordfish (55%) were the least frequent. A positive sIgE (value ≥ 0.35 kUA/L) to the culprit species ranged between 50% (swordfish) and 100% (hake). In tolerant patients, a negative sIgE (value allergy or tolerance was 82% and 25%, respectively. Sensitization to cod parvalbumin (Gad c 1) was present in 77% of all patients. Serological cross-reactivity between fish species is frequent, but in a significant proportion of patients, clinical relevance appears to be limited to only certain species. A well-taken history or food challenge is required for discrimination between allergy to the different fish species.

  11. Performance of a polymer coated silicon microarray for simultaneous detection of food allergen-specific IgE and IgG4.

    Science.gov (United States)

    Sievers, S; Cretich, M; Gagni, P; Ahrens, B; Grishina, G; Sampson, H A; Niggemann, B; Chiari, M; Beyer, K

    2017-08-01

    Microarray-based component-resolved diagnostics (CRD) has become an accepted tool to detect allergen-specific IgE sensitization towards hundreds of allergens in parallel from one drop of serum. Nevertheless, specificity and sensitivity as well as a simultaneous detection of allergen-specific IgG 4 , as a potential parameter for tolerance development, remain to be optimized. We applied the recently introduced silicon chip coated with a functional polymer named copoly(DMA-NAS-MAPS) to the simultaneous detection of food allergen-specific IgE and IgG 4 , and compared it with ImmunoCAP and ImmunoCAP ISAC. Inter- and intraslide variation, linearity of signal and working range, sensitivity and application of internal calibrations for IgE and IgG 4 were assessed. Native and recombinant allergenic proteins from hen's egg and cow's milk were spotted on silicon chips coated with copoly(DMA-NAS-MAPS) along with known concentrations for human IgE and IgG 4 . A serum pool and 105 patient samples were assessed quantitatively and semi-quantitatively with the ImmunoCAP and ImmunoCAP ISAC and correlated with IgE- and IgG 4 -specific fluorescence on silicon microarrays. Allergen-specific IgE and IgG 4 were detected in parallel using two fluorescent dyes with no crosstalk. Results from the ImmunoCAP correlated better with microarray fluorescence than with ImmunoCAP ISAC except for the allergen ovomucoid. The working range of the silicon microarray for total hen's egg-specific IgE was comparable to the range of 0.1 to >100 kU A /L of the ImmunoCAP system, whereas for total cow's milk, the silicon microarray was less sensitive. Detectable allergen-specific IgG 4 could be determined only for low concentrations, but still correlated positively with ImmunoCAP results. We confirmed the ability of the polymer coated silicon microarray to be comparably sensitive to the ImmunoCAP ISAC for various food allergens. This suggests that the copoly(DMA-NAS-MAPS) microarray is a low-cost, self

  12. Phthalate treatment does not influence levels of IgE or Th2 cytokines in B6C3F1 mice

    International Nuclear Information System (INIS)

    Butala, John H.; David, Raymond M.; Gans, Gerhard; McKee, Richard H.; Guo, Tai L.; Peachee, Vanessa L.; White, Kimber L.

    2004-01-01

    Bronchial asthma is mediated, in part, by the immunoregulatory cytokines interleukins 4 and 13 (IL-4 and IL-13). These cytokines stimulate IgE synthesis that in turn is associated with airway hyper-responsiveness. Compounds that stimulate IgE synthesis and elicit bronchial reactivity are generally considered to be respiratory sensitizers. Recently, it has been hypothesized that exposure to phthalates may contribute to childhood asthma. To address this question, di-(2-ethylhexyl) phthalate (DEHP) was tested using a protocol adapted from work by Dearman that involves topical application (and challenge) of test substances to mice followed by measurements of total serum IgE. In addition, auricular lymph nodes were harvested for measurement of IL-4 and IL-13 proteins and their corresponding messenger RNAs. Because skin absorption of high molecular weight phthalates is limited, liver weight increase, a measure of peroxisomal proliferation, was monitored to assure that internal dosing had been achieved. ELISA and RNAse protection assays demonstrated that DEHP treatment did not significantly affect IgE, IL-4, or IL-13 levels. Similarly, IL-4 and IL-13 mRNA levels were not elevated. In contrast, all of these were significantly elevated by trimellitic anhydride (TMA), a respiratory sensitizer used as the positive control in this assay. Liver weights were significantly elevated by DEHP, providing evidence of sufficient percutaneous absorption to induce physiological responses. To extend these observations, three other commercial phthalate ester plasticizers, di-isononyl phthalate (DINP), di-isohexyl phthalate (DIHP), and butyl benzyl phthalate (BBP), were assessed using the same protocol. As above, ELISA and RNAse protection assays showed that IgE, IL-4, and IL-13 proteins, and IL-4 and IL-13 mRNAs in the phthalate-treated animals were all at levels similar to that of control values. The positive control, TMA, produced large, statistically significant increases in all

  13. Positive serum specific IgE has a short half-life in patients with penicillin allergy and reversal does not always indicate tolerance.

    Science.gov (United States)

    Hjortlund, Janni; Mortz, Charlotte Gotthard; Stage, Tore Bjerregaard; Skov, Per Stahl; Dahl, Ronald; Bindslev-Jensen, Carsten

    2014-01-01

    The positive and negative predictive values of specific IgE to penicillins are not well established for penicillin hypersensitivity. One reason may be that serum IgE levels to penicillin diminish over time. The objective in this study was to investigate variations in serum half-life (T½) for specific IgE to penicillins (s-IgE) and to evaluate the outcome of penicillin challenges in patients with previous but not present specific IgE to penicillins. Two subgroups were investigated. All included patients had a history of penicillin allergy with reported symptoms such as urticaria/angioedema or unclassified cutaneous rash. T½ of specific IgE to penicillins was calculated based on sera from 29 patients with repeated measurements of s-IgE. Twenty-two patients with a previous positive s-IgE was followed and challenged with penicillin when IgE had become negative. The T½ for s-IgE varied between the 26 patients with decreasing s-IgE from 1.6 months to 76.4 months and 52% had a T½ of less than a year. The three patients with stable and increasing IgE-values showed T½ approaching infinity A total of 29 challenges with β-lactams were performed. Four different patterns were seen when evaluating the clinical reaction to challenge (positive/negative) and post-challenge boost of s-IgE (yes/no). Eight (36.4%) had negative challenge and negative post-challenge s-IgE, eight (36.4%) negative challenge, but positive post-challenge s-IgE levels. 3 (13.6%) had positive challenge and positive post-challenge s-IgE whereas 3 (13.6%) were challenge positive, but had negative post-challenge s-IgE. Specific IgE to penicillins declines over time stressing the importance of a close time relation between diagnostic work-up and clinical reaction. Reversal of previously positive s-IgE may still be associated with positive penicillin challenges and/or re-boostering of s-IgE to positivity.

  14. Asthma Heredity, Cord Blood IgE and Asthma-Related Symptoms and Medication in Adulthood: A Long-Term Follow-Up in a Swedish Birth Cohort.

    Directory of Open Access Journals (Sweden)

    Hartmut Vogt

    Full Text Available Cord blood IgE has previously been studied as a possible predictor of asthma and allergic diseases. Results from different studies have been contradictory, and most have focused on high-risk infants and early infancy. Few studies have followed their study population into adulthood. This study assessed whether cord blood IgE levels and a family history of asthma were associated with, and could predict, asthma medication and allergy-related respiratory symptoms in adults. A follow-up was carried out in a Swedish birth cohort comprising 1,701 consecutively born children. In all, 1,661 individuals could be linked to the Swedish Prescribed Drug Register and the Medical Birth Register, and 1,227 responded to a postal questionnaire. Cord blood IgE and family history of asthma were correlated with reported respiratory symptoms and dispensed asthma medication at 32-34 years. Elevated cord blood IgE was associated with a two- to threefold increased risk of pollen-induced respiratory symptoms and dispensed anti-inflammatory asthma medication. Similarly, a family history of asthma was associated with an increased risk of pollen-induced respiratory symptoms and anti-inflammatory medication. However, only 8% of the individuals with elevated cord blood IgE or a family history of asthma in infancy could be linked to current dispensation of anti-inflammatory asthma medication at follow-up. In all, 49 out of 60 individuals with dispensed anti-inflammatory asthma medication at 32-34 years of age had not been reported having asthma at previous check-ups of the cohort during childhood. Among those, only 5% with elevated cord blood IgE and 6% with a family history of asthma in infancy could be linked to current dispensation of anti-inflammatory asthma medication as adults. Elevated cord blood IgE and a positive family history of asthma were associated with reported respiratory symptoms and dispensed asthma medication in adulthood, but their predictive power was poor

  15. Increases in IgE, Eosinophils, and Mast Cells Can be Used in Diagnosis and to Predict Relapse of IgG4-Related Disease.

    Science.gov (United States)

    Culver, Emma L; Sadler, Ross; Bateman, Adrian C; Makuch, Mateusz; Cargill, Tamsin; Ferry, Berne; Aalberse, Rob; Barnes, Eleanor; Rispens, Theo

    2017-09-01

    IgG subclass 4-related disease (IgG4-RD) is characterized by increased serum levels of IgG4 and infiltration of biliary, pancreatic, and other tissues by IgG4-positive plasma cells. We assessed the prevalence of allergy and/or atopy, serum, and tissue IgE antibodies, and blood and tissue eosinophils in patients with IgG4-RD. We investigated the association between serum IgE and diagnosis and relapse of this disease. We performed a prospective study of 48 patients with IgG4-RD, 42 patients with an increased serum level of IgG4 with other inflammatory and autoimmune conditions (disease control subjects), and 51 healthy individuals (healthy control subjects) recruited from Oxford, United Kingdom from March 2010 through March 2014, and followed for a median of 41 months (range, 3-73 months). Serum levels of immunoglobulin were measured at diagnosis, during steroid treatment, and at disease relapse for patients with IgG4-RD; levels at diagnosis were compared with baseline levels of control subjects. Allergen-specific IgEs were measured using the IgE ImmunoCAP. Levels and distribution of IgG4 and IgE antibodies in lymphoid, biliary, and pancreatic tissues from patients with IgG4-RD and disease control subjects were measured by immunohistochemistry. We analyzed data using the Spearman rank correlation and receiver operating characteristic curves. Serum levels of IgG4 increased to 1.4 g/L or more, and IgE increased to 125 kIU/L or more, in 81% and 54% of patients with IgG4-RD, respectively, compared with 6% and 16% of healthy control subjects (P IgG4-RD versus 9% of healthy control subjects (P = .004). Of patients with IgG4-RD, 63% had a history of allergy and 40% had a history of atopy with an IgE-specific response; these values were 60% and 53% in patients with increased serum levels of IgE (P 480 kIU/L distinguished patients with IgG4-RD from disease control subjects with 86% specificity, 36% sensitivity, and a likelihood ratio of 3.2. Level of IgE at diagnosis >380 k

  16. Allergy to fish collagen: Thermostability of collagen and IgE reactivity of patients' sera with extracts of 11 species of bony and cartilaginous fish.

    Science.gov (United States)

    Kobayashi, Yukihiro; Kuriyama, Takuma; Nakagawara, Ryoko; Aihara, Michiko; Hamada-Sato, Naoko

    2016-10-01

    Parvalbumin was identified as a major fish allergen, and has been well investigated. Collagen was identified as a second allergen; however, its allergenic properties remain uncharacterized. Although fish is an important staple in coastal countries, its thermostability is unknown. Therefore, we aimed to determine the thermostability of fish collagen as an allergen. Meat of seven bony and four cartilaginous fishes was heated at various temperatures and times, and extracts were analyzed using SDS-PAGE, IgE-ELISA, and SPTs. Collagen was dissolved from heated meat of Pacific mackerel into a crude extract. Collagen in the extracts was degraded at a high heating load-140 °C (10 min) or 100 °C (320 min). However, ELISA revealed the IgE reactivities of patients' sera with the extracts were unchanged even after heating the samples. Patients strongly reacted to extract proteins of other bony fish, which were detected by patients' IgE even after heating at 100 °C (320 min). In contrast, reactivities of the extracts of cartilaginous fish were lower than those of bony fish. SPTs in one patient revealed that all bony and cartilaginous fish extracts prepared from heated meat elicited allergic reactions. The IgE reactivity of patients' sera to fish collagen in extracts was retained even when fish meat was treated by a high heating load. As for the fish collagen, the IgE reactivities to cartilaginous fish were lower than that to bony fish. Reducing IgE reactivity to fish meat using heat is difficult, and other modalities will be required to produce hypoallergenic fish meat. Copyright © 2016 Japanese Society of Allergology. Production and hosting by Elsevier B.V. All rights reserved.

  17. Specific IgE Antibodies in Young Children with Atopic Dermatitis--Correlation of Multiple Allergen Simultaneous Immunoblot Test and ImmunoCap System.

    Science.gov (United States)

    Konopka, Ewa; Ceregra, Aldona; Maciorkowska, Elzbieta; Surowska, Barbara; Trojanowska, Ilona; Roszko-Kirpsza, Izabela; Cukrowska, Bozena

    2016-01-01

    Sensitization to food allergens is a common condition in pediatric atopic dermatitis (AD). Recently, the multiple allergen simultaneous test (MAST) allowing for a comprehensive assessment of atopy has been developed, but the usefulness in young AD children is not known. The aim of this study was to determine IgE specificity in AD children using MAST and to compare the results for selected food allergens with the reference ImmunoCap system. The study enrolled 50 children up to 2 years old with a diagnosis of AD. IgE antibodies were measured with the MAST-immunoblots. Children with specific IgE levels ≥ 0.35 kU/L were identified as sensitized to allergens. Most often children were sensitized to food allergens (egg white and yolk, hazelnuts, potato, cow's milk proteins, wheat flour, codfish, and soybean), but a high percentage of them also had IgE antibodies against house dust mites (12%), grass (10%), and birch (10%). Eight percent of children were sensitized to domestic animals (cats and dogs). Almost perfect (kappa index 0.8 - 1.0) and substantial (kappa index 0.6 - 0.8) agreement between MAST and ImmunoCap was found for food allergens except codfish. Pearson's analysis of antibody classes showed a very strong correlation between two methods (r = 0.8 - 1.0) for egg white, hazelnuts, potato, cow's milk proteins, wheat flour, and soybean, and a strong correlation (r = 0.6 - 0.79) was observed for peanut, egg yolk, and codfish. The study showed the frequent occurrence of IgE antibodies against food and airborne and animal allergens in young AD children and confirmed the usefulness of MAST-immunoblots for screening of sensitization in pediatric patients.

  18. The Ethanol-Chlorobenzene Aerated System as a New High-Level Dosimeter for Routine Measurements; Le Systeme Ethanol-Chlorobenzene Aere Utilise Comme Dosimetre pour les Mesures Courantes de Fortes Doses; Aehrirovannaya sistema ehtanola-khlorobenzola v kachestve novogo dozimetra vysokogo urovnya dlya provedeniya obychnykh izmerenij; Dosimetro de Etanol-Clorobenceno Saturado de Aire para la Determinacion de Intensidades Elevadas

    Energy Technology Data Exchange (ETDEWEB)

    Dvornik, I.; Zec, U.; Ranogajec, F. [Institute ' ' Ruder Boskovic' ' , Zagreb, Yugoslavia (Croatia)

    1966-11-15

    resultados reproducibles, con ligeros porcentajes de desviacion respecto de este valor, anadiendo al principio de la operacion 4% de agua y 2 * 10{sup -3} M, aproximadamente, de benceno y acetaldehfdo o acetona. La exactitud de las mediciones es mayor si se determina el valor exacto de G(Cl{sup -}) para cada material por calibracion con el dosimetro de Fricke, con 4% de CB y 4% de agua todas las variedades de etanol dan G(Cl{sup -}) = 3,65 {+-} 0,05, con independencia de los aditivos. El efecto de la variacion del contenido de agua en {+-} 1% es insignificante. La concentracion del HC1 en todo el intervalo de dosis se puede determinar por valoracion mercurometrica o alcalimetrica directa de la muestra irradiada con un error maximo del 1%. Experimentos con neutrones rapidos, todavia en curso, demuestran que podria emplearse mezclas similares para calibrar y controlar irradiaciones industriales con haces electronicos intensos. La sencillez de la preparacion y del analisis junto con el amplio intervalo que se cubre con este solo metodo y con su elevada precision, hacen que el nuevo dosimetro sea uno de los mas eficaces para calibrar fuentes radiactivas y controlar procesos industriales de irradiacion. (author) [Russian] Obnaruzheno, chto vyhod radioliticheskogo hloristogo vodoroda dlja gamma-izluchenija kobal'ta-60, izmerennogo v kachestve funkcii dozy (0,05 - 100 Mrad), moshhnosti dozy (0,5 - 2500 rad/sek{sup 1}) i koncentracii hlorobenzola (HB), sostavljaet 5 {+-}0,1 dlja koncentracii HB 20-40% v shirokom diapazone jeksperimental'nyh uslovij. Ukazannyj vyhod ochen' malo zavisit ot obychnyh zagrjaznenij. Komponenty analiticheskoj chistoty i nizhe dajut vyhody, kotorye mogut byt' vosproizvedeny v predelah neskol'kih procentov jetoj velichiny, esli pervonachal'no imeetsja 4% vody i okolo 2*10{sup -3} M benzola i acetal'degida ili acetona. Bolee vysokuju dozimetricheskuju tochnost' mozhno poluchit' v tom sluchae, esli opredelit' tochno G(C1' ) dlja dannyh materialov putem

  19. Exantema fijo medicamentoso por paracetamol. Caso clínico con revisión bibliográfica

    Directory of Open Access Journals (Sweden)

    Justo Rueda López

    Full Text Available RESUMEN El exantema fijo medicamentoso es una toxicodermia caracterizada por la aparición de una lesión cutánea en forma de mácula de color rojizo o violáceo, redonda u oval, edematosa, bien delimitada, y que presenta recurrencias tras la administración del agente causal, generalmente un fármaco. Puede ser producido por una gran variedad de fármacos, entre ellos el paracetamol. El paracetamol es un medicamento con propiedades analgésicas y antipiréticas ampliamente conocido y utilizado por su amplio margen de seguridad y elevada biodisponibilidad. Sin embargo, se han descrito reacciones cutáneas adversas de diferentes tipos e intensidad tras su administración, fundamentalmente urticaria y angioedema, y con menor frecuencia eritema multiforme, necrólisis epidérmica tóxica, exantema fijo y púrpura de Henoch-Schönlein. Se presenta el caso de un usuario tipo con exantema fijo medicamentoso relacionado con el consumo de paracetamol. Se ha realizado una revisión bibliográfica sobre los diversos hallazgos clínicos y el diagnóstico diferencial entre las diferentes toxicodermias, así como el manejo y abordaje de las lesiones basado en el concepto TIME.

  20. Acne polimorfo: tratamiento con Implacen

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    Rubén Pérez Armas

    1995-06-01

    Full Text Available Se realiza el estudio de 40 pacientes con acné polimorfo, los que fueron atendidos en la Consulta de Dermatología del Hospital Provincial Clinicoquirúrgico Docente "Celia Sánchez Manduley", en el período comprendido de enero de 1988 a diciembre de 1989. Se revisa la literatura médica sobre los diversos métodos y medicamentos utilizados en la terapéutica de esta dermatosis. Se describe el esquema de tratamiento empleado con implacén en 30 pacientes; los 10 restantes se trataron con placebo; se compara dicho esquema con los tradicionales y se observan mejores resultados con nuestro estudio. Se destaca la ausencia de recaídas, así como el resultado del tratamiento de acuerdo con el sexo.A study was performed in 40 patients presenting with polymorphic acne who were attended in the Dermatology Department of "Celia Sánchez Manduley" Clinicosurgical and Teaching Hospital from January, 1988 to December, 1989. A review of the literature was made seeking for the different methods and drugs used for the treatment of this dermatosis. The treatment schedule with the use of implacen in 30 patients is described. Such therapeutic schedule was compared with traditional ones and better results were observed with the use of implacen. The fact that there were no relapses is highlighted, as well as the result of treatment according to sex.

  1. Dimetilarginina asimétrica plasmática en mujeres obesas y no obesas con síndrome de ovarios poliquísticos

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    Jorly Mejia Montilla

    2016-12-01

    Full Text Available OBJETIVO: Determinar las concentraciones plasmáticas de factor de dimetilarginina asimétrica (ADMA en mujeres obesas y no obesas con diagnóstico de síndrome de ovarios poliquísticos (SOPQ. MÉTODOS: Se realizó un estudio de casos y controles en mujeres con diagnóstico de SOPQ y controles sanas de edades similares, con menstruaciones regulares y ovarios normales por ecografía y fueron divididas en cuatro grupos (grupo A: SOPQ obesas; grupo B: SOPQ no obesas; grupo C: controles obesas y grupo D controles no obesas de acuerdo al índice de masa corporal (obesas > 30 Kg/m2 y no obesas < 25 kg/m2. Se midieron las concentraciones de hormonas sexuales, globulina fijadora de hormonas sexuales, glucosa sérica, insulina y ADMA. RESULTADOS: Las mujeres con SOPQ obesas y no obesas presentaron concentraciones más elevadas de hormonas sexuales e insulina comparadas con el grupo control de obesas y no obesas (p < 0,0001. Se observó que las mujeres con SOPQ presentaron concentraciones significativamente más altas de ADMA (grupo A: 0,56 +/- 0,05 picomol/L y grupo B: 0,51 +/- 0,03 picomol/L comparado con los controles (grupo C: 0,47 +/- 0,02 picomol/L y grupo D 0,45 +/- 0,04 picomol/L; p < 0,0001. Se observó que las concentraciones de ADMA presentaban correlación positiva y significativa con los valores de glicemia e insulina en ayunas en las mujeres con SOPQ (p < 0,0001. CONCLUSIÓN: Existen diferencias significativas en las concentraciones plasmáticas del ADMA entre las mujeres con SOPQ obesas y no obesas respecto a los controles normales.

  2. High correlation of specific IgE sensitization between birch pollen, soy and apple allergens indicates pollen-food allergy syndrome among birch pollen allergic patients in northern China.

    Science.gov (United States)

    Hao, Guo-Dong; Zheng, Yi-Wu; Wang, Zhi-Xiang; Kong, Xing-Ai; Song, Zhi-Jing; Lai, Xu-Xin; Spangfort, Michael D

    2016-05-01

    Birch pollen sensitization and associated pollen-food syndrome among Chinese allergic patients have not been investigated. Sera from 203 allergic patients from the northern part of China and collected during February to July 2014 were investigated. Specific immunoglobulin E (IgE) against birch pollen extract Bet v and major birch pollen allergen Bet v 1 were measured using the ADVIA Centaur. The presence of major apple allergen Mal d 1 and soy bean allergen Gly m 4 specific IgE was measured by ImmunoCAP 100. Among the 203 sera, 34 sera (16.7%) had specific IgE to Bet v and of these, 28 sera (82.4%) contained Bet v 1-specific IgE. Among the 28 sera with Bet v 1-specific IgE, 27 sera (96.4%) contained Mal d 1-specific IgE and 22 sera (78.6%) contained Gly m 4-specific IgE. Of the 34 Bet v-positive sera, 6 sera (17.6%) contained no specific IgE for Bet v 1, Mal d 1, or Gly m 4. Almost all Bet v-positive sera were donated during the birch pollen season. The prevalence of birch allergy among patients visiting health care during pollen season can be as high as 16.7% in Tangshan City. The majority of Chinese birch allergic patients are IgE-sensitized to the major birch pollen allergen Bet v 1 as well as to the major apple allergen Mal d 1 and soy bean allergen Gly m 4. A relatively high number of patients (17.6%) are IgE-sensitized to birch pollen allergen(s) other than Bet v 1. The high prevalence of specific IgE to Mal d 1 and Gly m 4 among Bet v 1-sensitized patients indicates that pollen-food allergy syndrome could be of clinical relevance in China.

  3. con mala calidad de vida

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    Agustín Martín-Rodríguez

    2007-01-01

    Full Text Available En este estudio ex post facto se ha analizado si los familiares de pacientes con mala calidad de vida presentan diferencias en las variables clínicas de personalidad y relaciones familiares en función de que el paciente haya estado o no ingresado en una Unidad de Cuidados Intensivos. Seleccionamos dos grupos: 29 familiares de pacientes traumatizados graves transcurridos cuatro años de su ingreso en una UCI de Traumatología y con mala calidad de vida (debido a secuelas físicas y/o psicológicas tras el ingreso, tales como traumatismos craneoencefálicos, politraumatismos y tetraplejias traumáticas y 32 familiares de pacientes con mala calidad de vida con cuatro años de evolución de su enfermedad física (hipertensión, diabetes, artritis reumatoide y síndrome de intestino irritable que no han estado ingresados en la UCI. Para alcanzar nuestro objetivo empleamos una Encuesta Psicosocial y los siguientes instrumentos: Cuestionario de Análisis Clínico, Escala de Clima Social en la Familia y Escala de Adaptación Psicosocial de la Enfermedad. Los resultados mostraron que los familiares de pacientes con mala calidad de vida que estuvieron ingresados en la UCI hace cuatro años, presentan diferencias significativas en las variables agitación y expresividad comparados con los familiares de pacientes con mala calidad de vida que no han estado ingresados en la UCI.

  4. Contacto precoz con la realidad asistencial: una experiencia piloto en medicina

    Directory of Open Access Journals (Sweden)

    J.E. Baños

    Full Text Available Introducción: Varios estudios han sugerido que el contacto precoz del estudiante de medicina con la realidad asistencial puede tener unos efectos beneficiosos sobre su motivación, el conocimiento de la relación médico-paciente y la aceptación de la importancia de las materias médicas básicas. En el nuevo grado conjunto de Medicina de la Universitat Autònoma de Barcelona y la Universitat Pompeu Fabra se ha incorporado una asignatura denominada 'Prácticas de Grado' que se imparte durante los tres primeros años, destinada a permitir el contacto de los estudiantes con la asistencia primaria, los equipos de enfermería y los centros sociosanitarios. El presente artículo describe la experiencia y la opinión de los estudiantes que la cursaron durante el primer año. Materiales y métodos: El artículo describe las características académicas de la asignatura. Se realizó una encuesta a los estudiantes al finalizar la asignatura para evaluar su grado de satisfacción y su percepción sobre el cumplimiento de los objetivos y las competencias que debían alcanzarse. Resultados: Existió una elevada satisfacción con la nueva actividad y una percepción entre los estudiantes de que las competencias preestablecidas se habían alcanzado en su mayor parte. Conclusión: El contacto precoz con la realidad asistencial es un elemento esencial para la comprensión de la actividad médica por los estudiantes de medicina de primer año.

  5. Close-up of the alpha-1,3-Gal epitope as defined by a monoclonal chimeric IgE and human serum using saturation transfer difference (STD) NMR

    DEFF Research Database (Denmark)

    Plum, Melanie; Michel, Yvonne; Wallach, Katharina

    2011-01-01

    of an alpha-Gal-specific murine IgM antibody was employed to construct chimeric IgE and IgG antibodies. Reactivity and specificity of the resulting antibodies were assessed by means of ELISA and receptor binding studies. Using defined carbohydrates, interaction of the IgE and human serum was assessed...... by mediator release assays, surface plasmon resonance (SPR) and STD NMR analyses. The alpha-Gal-specific chimeric IgE and IgG antibodies were proven functional regarding interaction with antigen and Fc receptors. SPR measurements demonstrated affinities in the micromolar range. In contrast to a reference...

  6. Evidence for a peak shift in a humoral response to helminths: age profiles of IgE in the Shuar of Ecuador, the Tsimane of Bolivia, and the U.S. NHANES.

    Directory of Open Access Journals (Sweden)

    Aaron D Blackwell

    2011-06-01

    Full Text Available The peak shift model predicts that the age-profile of a pathogen's prevalence depends upon its transmission rate, peaking earlier in populations with higher transmission and declining as partial immunity is acquired. Helminth infections are associated with increased immunoglobulin E (IgE, which may convey partial immunity and influence the peak shift. Although studies have noted peak shifts in helminths, corresponding peak shifts in total IgE have not been investigated, nor has the age-patterning been carefully examined across populations. We test for differences in the age-patterning of IgE between two South American forager-horticulturalist populations and the United States: the Tsimane of Bolivia (n=832, the Shuar of Ecuador (n=289, and the U.S. NHANES (n=8,336. We then examine the relationship between total IgE and helminth prevalences in the Tsimane.Total IgE levels were assessed in serum and dried blood spots and age-patterns examined with non-linear regression models. Tsimane had the highest IgE (geometric mean =8,182 IU/ml, followed by Shuar (1,252 IU/ml, and NHANES (52 IU/ml. Consistent with predictions, higher population IgE was associated with steeper increases at early ages and earlier peaks: Tsimane IgE peaked at 7 years, Shuar at 10 years, and NHANES at 17 years. For Tsimane, the age-pattern was compared with fecal helminth prevalences. Overall, 57% had detectable eggs or larva, with hookworm (45.4% and Ascaris lumbricoides (19.9% the most prevalent. The peak in total IgE occurred around the peak in A. lumbricoides, which was associated with higher IgE in children <10, but with lower IgE in adolescents.The age-patterning suggests a peak shift in total IgE similar to that seen in helminth infections, particularly A. lumbricoides. This age-patterning may have implications for understanding the effects of helminths on other health outcomes, such as allergy, growth, and response to childhood vaccination.

  7. Evidence for a peak shift in a humoral response to helminths: age profiles of IgE in the Shuar of Ecuador, the Tsimane of Bolivia, and the U.S. NHANES.

    Science.gov (United States)

    Blackwell, Aaron D; Gurven, Michael D; Sugiyama, Lawrence S; Madimenos, Felicia C; Liebert, Melissa A; Martin, Melanie A; Kaplan, Hillard S; Snodgrass, J Josh

    2011-06-01

    The peak shift model predicts that the age-profile of a pathogen's prevalence depends upon its transmission rate, peaking earlier in populations with higher transmission and declining as partial immunity is acquired. Helminth infections are associated with increased immunoglobulin E (IgE), which may convey partial immunity and influence the peak shift. Although studies have noted peak shifts in helminths, corresponding peak shifts in total IgE have not been investigated, nor has the age-patterning been carefully examined across populations. We test for differences in the age-patterning of IgE between two South American forager-horticulturalist populations and the United States: the Tsimane of Bolivia (n=832), the Shuar of Ecuador (n=289), and the U.S. NHANES (n=8,336). We then examine the relationship between total IgE and helminth prevalences in the Tsimane. Total IgE levels were assessed in serum and dried blood spots and age-patterns examined with non-linear regression models. Tsimane had the highest IgE (geometric mean =8,182 IU/ml), followed by Shuar (1,252 IU/ml), and NHANES (52 IU/ml). Consistent with predictions, higher population IgE was associated with steeper increases at early ages and earlier peaks: Tsimane IgE peaked at 7 years, Shuar at 10 years, and NHANES at 17 years. For Tsimane, the age-pattern was compared with fecal helminth prevalences. Overall, 57% had detectable eggs or larva, with hookworm (45.4%) and Ascaris lumbricoides (19.9%) the most prevalent. The peak in total IgE occurred around the peak in A. lumbricoides, which was associated with higher IgE in children <10, but with lower IgE in adolescents. The age-patterning suggests a peak shift in total IgE similar to that seen in helminth infections, particularly A. lumbricoides. This age-patterning may have implications for understanding the effects of helminths on other health outcomes, such as allergy, growth, and response to childhood vaccination.

  8. Heterologous antigen extract in ELISA for the detection of human IgE anti-Strongyloides stercoralis Extrato antigênico heterólogo em ELISA para a detecção de IgE humana anti-Strongyloides stercoralis

    Directory of Open Access Journals (Sweden)

    Julia Maria Costa-Cruz

    2003-10-01

    Full Text Available Strongyloides ratti larval extract was used for the standardization of ELISA to detect genus-specific IgE in human strongyloidiasis. Forty serum samples from monoinfected patients shedding S. stercoralis larvae (Group I, 40 from patients with other intestinal parasites (Group II, and 40 from copronegative healthy subjects (Group III were analyzed. Genus-specific IgE levels (ELISA Index: EI were significantly higher in the group I (EI = 1.43 than groups II (EI = 0.70 and III (EI = 0.71, showing positivity rates of 55%, 2.5% and 0%, respectively. Similarly, sera from copropositive patients had significantly higher levels of total IgE (866 IU/mL as compared to those from group II (302 IU/mL and III (143 IU/mL. A significant positive correlation was found between levels of Strongyloides specific-IgE and total IgE in sera from patients with strongyloidiasis. In conclusion, S. ratti heterologous extract showed to be a useful tool for detecting genus-specific IgE by ELISA, contributing for a better characterization of the immune response profile in human strongyloidiasis.Extrato contendo larvas de Strongyloides ratti foi usado na padronização de um ELISA para detecção de IgE gênero-específica na estrongiloidíase humana. Foram analisadas 40 amostras de soro de pacientes monoinfectados que estavam eliminando larvas de S. stercoralis nas fezes (Grupo I, 40 de pacientes com outros parasitos intestinais (Grupo II, e 40 indivíduos copronegativos (Grupo III. Níveis de IgE gênero-específica (índice ELISA: EI foram significativamente maiores no Grupo I (EI = 1,43 do que no II (EI = 0,70 e III (EI = 0,71, mostrando positividade de 55%, 2,5% e 0%, respectivamente. Similarmente, soros dos pacientes copropositivos (Grupo I apresentaram níveis significativamente maiores de IgE total (866 IU/mL quando comparados com os soros dos Grupo II (302 IU/mL e III (143 IU/mL. Uma significativa correlação positiva foi encontrada entre os níveis de IgE espec

  9. Seroprevalencia del virus linfotropo T humano tipo 1 (HTLV-1 en gestantes y grupos de elevada prevalencia para enfermedades de transmisión sexual de Ayacucho, Perú

    Directory of Open Access Journals (Sweden)

    Zoraida Juscamaita P

    2004-10-01

    Full Text Available Mediante encuestas sero-epidemiológicas se determinó la prevalencia de HTLV-1 en gestantes que acudieron a control prenatal al Hospital Regional de Ayacucho, ciudad andina del Perú, y en grupos de alta prevalencia para enfermedades de transmisión sexual (GEPETS. Las muestras séricas fueron tamizadas con el método de ELISA y finalmente confirmadas con el método de inmunoensayo en línea (INNO-LIA. Se enroló a 602 gestantes de las cuales 3 fueron positivas (0,5% y 3 indeterminadas (0,5. Todas las 85 trabajadoras sexuales fueron negativas, mientras que 2 de 74 homo/bisexuales (2,7% fueron indeterminados. En conclusión, la seroprevalencia de HTLV-1 en gestantes de Huamanga fue baja. La ausencia de casos positivos en los GEPETS contrasta con lo informado en nuestro país.

  10. IgE sensitization to food allergens and airborne allergens in relation to biomarkers of type 2 inflammation in asthma.

    Science.gov (United States)

    Patelis, A; Alving, K; Middelveld, R; James, A; Ono, J; Ohta, S; Izuhara, K; Borres, M P; Forsberg, B; Janson, C; Malinovschi, A

    2018-05-10

    We have recently reported that sensitization to food allergens and sensitization to airborne allergens had independent associations with increased fraction of exhaled nitric oxide (FeNO) and blood eosinophils in middle-aged adults and in young subjects with asthma. To investigate the relation between IgE sensitization and several type 2 inflammation biomarkers in adult asthmatics. FeNO, urinary eosinophil-derived neurotoxin (U-EDN), serum eosinophil cationic protein (S-ECP) and periostin were measured in 396 asthmatics, aged 17-76 years, from the Swedish GA2LEN study. Sensitization to airborne allergens was examined with skin prick tests (≥3 mm wheal) and sensitization to food allergens with measurement of specific IgE (≥0.35 kU/L). Asthmatics sensitized to food allergens had higher FeNO, 22.3 ppb (18.6, 26.7) vs 16.1 ppb (14.2, 18.2) (P = .005), S-ECP, 17.7 mg/L (14.8, 21.1) vs 12.8 mg/L (10.9, 14.9) (P = .01), and periostin, 73.7 (67.5, 80.3) ng/mL vs 59.9 (55.8, 64.2) ng/mL (P = .003), than non-sensitized subjects. Periostin levels in this group were also significantly higher than in the group sensitized only to airborne allergens (P = .01). Sensitization to food allergens related independently to FeNO (P = .02), S-ECP (P = .006) and periostin (P = .004), whereas sensitization only to airborne allergens related only to FeNO (P = .02) after adjustments for age, sex, height, weight and smoking history. FeNO correlated weakly with S-ECP (r = .17, P < .001), periostin (r = .19, P < .001) and U-EDN (0.16, P < .001). S-ECP also correlated weakly with U-EDN (r = .12, P = .02). None of the correlations between the remaining pairs of markers of type 2 inflammation were significant. Sensitization to food allergens related to several local and systemic type 2 inflammation markers, such as FeNO, S-ECP and periostin. Assessing the profile of allergic sensitization, including to food allergens, might improve the understanding and

  11. Identification of critical amino acids in the IgE epitopes of Ric c 1 and Ric c 3 and the application of glutamic acid as an IgE blocker.

    Directory of Open Access Journals (Sweden)

    Natalia Deus-de-Oliveira

    Full Text Available BACKGROUND: The allergenicity of Ricinus communis L. (castor bean, Euphorbiaceae is associated with components of its seeds and pollen. Castor bean allergy has been described not only in laboratory workers, but also in personnel working in oil processing mills, fertilizer retail, the upholstery industry and other industrial fields. In the present study, we describe the critical amino acids in the IgE-binding epitopes in Ric c 1 and Ric c 3, two major allergens of R. communis. In addition, we also investigate the cross-reactivity between castor bean and some air and food allergen extracts commonly used in allergy diagnosis. METHODOLOGY/PRINCIPAL FINDINGS: The IgE reactivity of human sera from atopic patients was screened by immune-dot blot against castor bean allergens. Allergenic activity was evaluated in vitro using a rat mast cell activation assay and by ELISA. Cross-reactivity was observed between castor bean allergens and extracts from shrimp, fish, gluten, wheat, soybean, peanut, corn, house dust, tobacco and airborne fungal allergens. We observed that treatment of rat and human sera (from atopic patients with glutamic acid reduced the IgE-epitope interaction. CONCLUSIONS/SIGNIFICANCE: The identification of glutamic acid residues with critical roles in IgE-binding to Ric c 3 and Ric c 1 support the potential use of free amino acids in allergy treatment.

  12. Identification of critical amino acids in the IgE epitopes of Ric c 1 and Ric c 3 and the application of glutamic acid as an IgE blocker.

    Science.gov (United States)

    Deus-de-Oliveira, Natalia; Felix, Shayany P; Carrielo-Gama, Camila; Fernandes, Keysson V; DaMatta, Renato Augusto; Machado, Olga L T

    2011-01-01

    The allergenicity of Ricinus communis L. (castor bean, Euphorbiaceae) is associated with components of its seeds and pollen. Castor bean allergy has been described not only in laboratory workers, but also in personnel working in oil processing mills, fertilizer retail, the upholstery industry and other industrial fields. In the present study, we describe the critical amino acids in the IgE-binding epitopes in Ric c 1 and Ric c 3, two major allergens of R. communis. In addition, we also investigate the cross-reactivity between castor bean and some air and food allergen extracts commonly used in allergy diagnosis. The IgE reactivity of human sera from atopic patients was screened by immune-dot blot against castor bean allergens. Allergenic activity was evaluated in vitro using a rat mast cell activation assay and by ELISA. Cross-reactivity was observed between castor bean allergens and extracts from shrimp, fish, gluten, wheat, soybean, peanut, corn, house dust, tobacco and airborne fungal allergens. We observed that treatment of rat and human sera (from atopic patients) with glutamic acid reduced the IgE-epitope interaction. The identification of glutamic acid residues with critical roles in IgE-binding to Ric c 3 and Ric c 1 support the potential use of free amino acids in allergy treatment.

  13. Tratamiento con implantes Leader-Nano en paciente con oligodoncia

    Directory of Open Access Journals (Sweden)

    Salvador Javier Santos Medina

    2015-03-01

    Full Text Available Los implantes dentales de titanio han revolucionado el mundo de la rehabilitación desde su surgimiento. De manera particular, el empleo de implantes de carga inmediata acorta el tiempo quirúrgico y protésico, con el consiguiente bienestar estético. Se presenta el caso de una paciente femenina de 32 años de edad, con antecedentes de oligodoncia de ambos incisivos laterales superiores y portadora de prótesis parcial acrílica. Fue atendida por el equipo multidisciplinario de implantes en la Clínica Estomatológica Docente “3 de Octubre” y se le realizó tratamiento de rehabilitación integral con implantes Leader-Nano y prótesis fija con corona acrílica sobre dichos implantes. La implantología fue satisfactoria en la paciente; la mejoría estética y funcional, así como la satisfacción de la paciente, fueron los principales logros obtenidos

  14. Frecuencia del síndrome de plaquetas pegajosas en pacientes con pérdidas fetales recurrentes

    Directory of Open Access Journals (Sweden)

    Loreta M Rodríguez-Pérez

    Full Text Available El síndrome de las plaquetas pegajosas es un trastorno plaquetario autosómico dominante considerado como una de las causas más frecuentes de eventos trombóticos. Se cree que el defecto específico puede estar localizado en los receptores de la superficie plaquetaria y está caracterizado por un incremento anormal en la agregabilidad de las plaquetas con difosfato de adenosina, epinefrina, o ambos. En estudios realizados acerca de esta entidad se ha descrito una elevada incidencia, pero esta se ha calculado en pacientes que han tenido episodios trombóticos previos (enfermedad tromboembólica, infarto agudo del miocardio y accidente cerebrovascular, antecedentes familiares o tienen factores de riesgo para este tipo de eventos, mientras que su incidencia en una población de mujeres en edad fértil, con dos o más pérdidas de embarazo y sin antecedentes es desconocida. En el presente trabajo se estudiaron un total de 126 pacientes femeninas con al menos dos pérdidas de embarazo; de ellas, 27 resultaron positivas al estudio de hipersensibilidad plaquetaria con difosfato de adenosina y epinefrina mediante pruebas de agregometría, lo que representa el 21 % de positividad en la población estudiada. Es significativo que la clase de síndrome de plaqueta pegajosa predominante fue de tipo II (hipersensibilidad con epinefrina. Finalmente, destacar que el seguimiento y tratamiento oportuno durante la gestación en las pacientes incluidas en el estudio ha permitido el nacimiento de 4 niños saludables de madres con síndrome de plaquetas pegajosas.

  15. Mitigación de la toxicidad anaerobia de lixiviados mediante mezclas con agua residual doméstica

    Directory of Open Access Journals (Sweden)

    Patricia Torres

    2010-01-01

    Full Text Available Los lixiviados contienen compuestos originalmente presentes en los residuos depositados en el relleno así como los formados en los procesos de degradación; cuando presentan altos contenidos de material orgánico y alta relación DBO5/DQO, el tratamiento biológico tiene gran potencial de aplicación; sin embargo, los altos niveles de nitrógeno y otros compuestos pueden ser inhibitorios o tóxicos para el proceso biológico. La evaluación de la toxicidad anaerobia permite determinar la magnitud y tipo de inhibición causada sobre los microorganismos encargados de la transformación final a metano y se determina comparando la Actividad Metanogénica Específica (AME del lodo control alimentado únicamente con sustrato, con la AME del lodo alimentado con el mismo sustrato más el compuesto tóxico. Dado el creciente interés en el cotratamiento de lixiviados con aguas residuales domésticas (ARD, se evaluó en escala de laboratorio la toxicidad anaerobia de lixiviados puros y mezclados con ARD para establecer el potencial efecto tóxico del lixiviado sobre la digestión anaerobia. En los ensayos usando lixiviado puro, se presentó una elevada reducción de la AME, identificándose dos tipos de inhibición: metabólica y fisiológica. En los ensayos usando mezclas de lixiviado y ARD (5 y 10%, el porcentaje de inhibición se redujo sustancialmente, alcanzándose condiciones de sustratos no tóxicos. Los resultados sugieren la potencialidad de aplicación del cotratamiento con ARD como una estrategia adecuada de manejo de lixiviados.

  16. Morbimortalidad cardiopulmonar en pacientes con lupus eritematoso sistémico

    Directory of Open Access Journals (Sweden)

    Alicia Aquino Valdovinos

    2015-08-01

    Full Text Available Los pacientes con diagnóstico de lupus eritematoso sistémico (LES presentan una elevada morbi-mortalidad asociada a las manifestaciones cardiopulmonares que se desarrollan durante la evolución de la enfermedad. En este sentido, se han descrito dos picos de incidencia de mortalidad de esta enfermedad inmunológica. El primer pico está relacionado a la actividad de la enfermedad y se observa durante los tres primeros años posteriores al diagnóstico. El segundo pico está relacionado principalmente a las complicaciones cardiopulmonares y se presenta entre los 4 y 20 años posteriores al diagnóstico. Si bien la mortalidad temprana ha disminuido gracias a un mayor conocimiento de la fisiopatología de la enfermedad y al uso de terapias inmunosupresoras, la mortalidad tardía presenta un aumento progresivo de su frecuencia a pesar del avance en el manejo terapéutico. El estudio de estas complicaciones se ha convertido en un tema de gran interés debido a su efecto negativo en el pronóstico de los pacientes que las presentan. Algunos estudios epidemiológicos sugieren que los pacientes con LES tienen un mayor riesgo cardiovascular al ser comparados con la población sana. En este sentido, se ha descrito que estos presentan un mayor riesgo de desarrollar eventos cardiovasculares (infarto agudo del miocardio, accidente cerebro vascular y enfermedad arterial periférica en comparación con la población general. De forma asociada, se ha objetivado que la presencia de manifestaciones pleuro-pulmonares (pleuritis lúpica, neumonitis, hemorragia pulmonar, embolismo pulmonar e hipertensión pulmonar aumenta la morbimortalidad de los pacientes con LES. El conocimiento adecuado de las complicaciones cardiopulmonares en los pacientes con diagnóstico de lupus eritematoso sistémico permitirá realizar un manejo individualizado y por lo tanto más eficaz, permitiendo disminuir la morbilidad asociada a las citadas complicaciones. El objetivo de este

  17. Detección de alteraciones numéricas en el gen dys y su asociación con rasgos clínicos Numeric alterations in the dys gene and their association with clinical features

    OpenAIRE

    Alejandra Mampel; María Inés Echeverría; Ana Lía Vargas; María Roque

    2011-01-01

    La distrofia muscular de Duchenne/Becker (DMD/B) es una miopatía hereditaria grave y progresiva. Se relaciona con alteraciones en el gen DYS, ubicado en el cromosoma X, que codifica para la proteína distrofina. Distintas manifestaciones pueden observarse según el impacto de la alteración genética sobre la proteína. Los registros internacionales de mutaciones refieren una elevada frecuencia (65-70%) de deleciones/duplicaciones de uno o más exones del gen DYS. En este trabajo presentamos el est...

  18. Influencia del entorno acústico laboral en el comportamiento audiométrico y su correlación con el registro de otoemisiones acústicas de productos de distorsión

    OpenAIRE

    Burgos Sánchez, Antonio Jesús

    2015-01-01

    El ruido puede ser considerado el contaminante físico con mayor presencia en el mundo laboral. La exposición continuada sin la protección adecuada, a niveles sonoros de elevada intensidad constituye ineludiblemente un riesgo potencial para la salud. El efecto nocivo del ruido en el medio laboral se traduce en una pérdida auditiva o hipoacusia que generalmente es progresiva e irreversible, y que estaría encuadrada dentro de la denominada pérdida auditiva inducida por ruido (PAIR). La evaluació...

  19. con dietas suplementadas con Cromo-L-metionina

    Directory of Open Access Journals (Sweden)

    Ram\\u00F3n Garc\\u00EDa-Castillo

    2006-01-01

    Full Text Available Un total de 48 cerdos (Sus scrofa domesticus; 24 machos castrados y 24 hembras cruzados (Yorkshire, Hampshire, Duroc y Landrace de 3,5 a 4,0 meses de edad y 60,0 ± 5,0 kg PV en finalización. Se alimentaron con dietas isoproteícas (14,5 % PC e isoenergéticas (3.400 kcal EM/kg de MS, adicionadas con Cr-L-metionina (MiCroPlex® (0, 200, 400 y 600 ppb. El experimento tuvo una duración de 45 días y se realizó de agosto a noviembre del 2002 en las instalaciones de la Universidad Autónoma Agraria Antonio Narro, localizada en Saltillo, Coahuila, México. Al tener los animales aproximadamente 95 kg PV, se tomó muestra de 15 ml de sangre por cada animal para determinar la concentración de glucosa, ácido úrico, creatinina, urea, proteinas totales y colesterol. Se aplicó un diseño completamente al azar con arreglo factorial 2 x 4; dos para el factor sexo y cuatro para nivel de cromo. Los metabolitos en suero no fueron afectados (P>0,05 por el factor sexo. La glucosa en suero disminuyó (P<0,05 y el colesterol incrementó (P<0,05 con cromo en la dieta. Se concluye que el Cr incrementa el metabolismo de glucosa y disminuye el de colesterol, con lo cual puede haber energía disponible para síntesis de proteína la cual es necesaria para el crecimiento de los animales

  20. The Comparison of Salivary IgA and IgE Levels in Children with Breast- and Formula- Feeding During Infancy Period

    Directory of Open Access Journals (Sweden)

    A. Jafarzadeh

    2007-01-01

    Full Text Available Introduction: Oral local immune factors may play a protective role against oral diseases and defend against microbial agents. Salivary immunoglobulin A (IgA is a major factor for the local host defence against caries and periodontal disease. The aims of this study were to determine the concentrations of salivary IgA and IgE levels in breast-fed and formula-fed children in infancy period.Methods and Materials: Totally, 80 healthy 5 years old children were included in the study. According to type of feeding in infancy period, the children divided into two groups: 50 breast-fed and 30 formula-fed. One milliliter of saliva was collected from each participant, centrifuged, and stored at -70 C. The salivary IgA and IgE concentrations were measured, using ELISA technique.Results: In breast-fed children, the salivary IgA level (39.6 mg/l ± 17.3 was significantly higher than that in formula-fed children (26.9 mg/l ± 14 (P=0.0001. However, the salivary IgE level was significantly lower in breast-fed children, comparing with formula fed ones (5.01 IU/ml ± 19.70 vs. 11.74 IU/ml ± 39.40 (P=0.047.Discussion: These results suggest that breast feeding enhances salivary IgA level in the early period of life which may contribute in oral cavity immunity. Higher salivary IgE level observed in formula-fed subjects may have a potential role in development of allergic or inflammatory reactions.

  1. Second-hand Smoke Increases Nitric Oxide and Alters the IgE Response in a Murine Model of Allergic Aspergillosis

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    Brian W. P. Seymour

    2005-01-01

    Full Text Available This study was performed to determine the effects of environmental tobacco smoke (ETS on nitric oxide (NO and immunoglobulin (Ig production in a murine model of allergic bronchopulmonary aspergillosis (ABPA. Adult BALB/c mice were exposed to aged and diluted sidestream cigarette smoke from day 0 through day 43 to simulate “second-hand smoke”. During exposure, mice were sensitized to soluble Aspergillus fumigatus (Af antigen intranasally between day 14 and 24. All Af sensitized mice in ambient air (Af + AIR made elevated levels of IgE, IgG1, IgM, IgG2a and IgA. Af sensitized mice housed in ETS (Af + ETS made similar levels of immunoglobulins except for IgE that was significantly reduced in the serum and bronchoalveolar lavage (BAL. However, immunohistochemical evaluation of the lung revealed a marked accumulation of IgE positive cells in the lung parenchyma of these Af + ETS mice. LPS stimulation of BAL cells revealed elevated levels of NO in the Af + AIR group, which was further enhanced in the Af+ETS group. In vitro restimulation of the BAL cells on day 45 showed a TH0 response with elevated levels of IL3, 4, 5, 10 and IFN-γ. However, by day 28 the response shifted such that TH2 cytokines increased while IFN-γ decreased. The Af + ETS group showed markedly reduced levels in all cytokines tested, including the inflammatory cytokine IL6, when compared to the Af+AIR group. These results demonstrate that ETS affects ABPA by further enhancing the NO production and reduces the TH2 and the inflammatory cytokines while altering the pattern of IgE responses.

  2. Serum concentrations of allergen-specific IgE in horses with equine recurrent airway obstruction and healthy controls assessed by ELISA.

    Science.gov (United States)

    Niedzwiedz, Artur; Jaworski, Zbigniew; Kubiak, Krzysztof

    2015-09-01

    Equine recurrent airway obstruction (RAO), also known as heaves, is one of the most common respiratory problems in older horses. When RAO-affected horses stay pastured or in a dust-free environment for a prolonged time, clinical signs as well as airway inflammation wane. A number of environmental, immunologic, infectious, and genetic factors play an important role in the pathogenesis of RAO, and the immunologic basis of this disease is still poorly understood. The aim of this study was to investigate the concentrations of allergen-specific IgE in the serum of horses suffering from RAO and healthy controls. The study included a group of 14 adult Polish Konik horses, kept in a standardized environment, and divided into 2 groups: 7 horses which did not have any respiratory problems comprised the control group and 7 horses with a history of RAO constituted the study group. A clinical and laboratory evaluation, endoscopic examination, and bronchoalveolar lavage (BAL) were performed in all horses. Sera of all horses were tested against allergens from 9 molds and 3 mites using the Heska Allercept assay. In the serologic tests, a statistically significant difference between both groups was found for specific IgE against mites, wherein Tyrophagus putrescentia correlated most clearly with RAO. There was no difference between groups for IgE specific against molds. On the basis of our observations and results, we conclude that RAO is associated with increased serum concentrations of specific serum IgE against mites, in particular T putrescentia. © 2015 American Society for Veterinary Clinical Pathology.

  3. Work-related allergy and asthma in spice mill workers - The impact of processing dried spices on IgE reactivity patterns.

    Science.gov (United States)

    van der Walt, Anita; Lopata, Andreas L; Nieuwenhuizen, Natalie E; Jeebhay, Mohamed F

    2010-01-01

    Three spice mill workers developed work-related allergy and asthma after prolonged exposure to high levels (>10 mg/m(3)) of inhalable spice dust. Patterns of sensitization to a variety of spices and putative allergens were identified. Work-related allergy and asthma were assessed on history, clinical evaluation, pulmonary function and fractional exhaled nitric oxide. Specific IgE reactivity to a range of common inhalant, food and spice allergens was evaluated using ImmunoCAP and allergen microarray. The presence of non-IgE-mediated reactions was determined by basophil stimulation (CAST-ELISA). Specific allergens were identified by immunoblotting to extracts of raw and dried processed garlic, onion and chili pepper. Asthma was confirmed in all 3 subjects, with work-related patterns prominent in worker 1 and 3. Sensitization to multiple spices and pollen was observed in both atopic workers 1 and 2, whereas garlic and chili pepper sensitization featured in all 3 workers. Microarray analysis demonstrated prominent profilin reactivity in atopic worker 2. Immunoblotting demonstrated a 50-kDa cross-reactive allergen in garlic and onion, and allergens of approximately 40 and 52 kDa in chili pepper. Dry powdered garlic and onion demonstrated greater IgE binding. This study demonstrated IgE reactivity to multiple spice allergens in workers exposed to high levels of inhalable spice dust. Processed garlic and onion powder demonstrated stronger IgE reactivity than the raw plant. Atopy and polysensitization to various plant profilins, suggesting pollen-food syndrome, represent additional risk factors for sensitizer-induced work-related asthma in spice mill workers. 2010 S. Karger AG, Basel.

  4. Toll-like receptor stimulation induces higher TNF-alpha secretion in peripheral blood mononuclear cells from patients with hyper IgE syndrome

    OpenAIRE

    Yeganeh, Mehdi; Henneke, Philipp; Rezaei, Nima; Ehl, Stephan; Thiel, Doerte; Matamoros, Nuria; Pietrogrande, Cristina; Espanol, Teresa; Litzman, Jiri; Franco, Jose L.; Sanal, Ozden; Kilic, Sara S.; Breborowicz, Anna; Plebani, Alessandro; Renner, Ellen

    2008-01-01

    Hyper IgE syndromes (HIES) are primary immunodeficiency disorders of unknown pathogenesis. Patients are typically affected with `cold' abscesses of the skin, recurrent cyst-forming pneumonia, chronic mucocutaneous candidiasis and other less frequent features such as progressive skeletal abnormalities. Defective signaling in the Toll-like receptor (TLR) pathways has been suggested as a responsible pathologic mechanism, however, in previous reports, 10 patients revealed no defect in inflammator...

  5. Diffuse alopecia areata is associated with intense inflammatory infiltration and CD8+ T cells in hair loss regions and an increase in serum IgE level

    Directory of Open Access Journals (Sweden)

    Ying Zhao

    2012-01-01

    Full Text Available Background: Mechanism leading to an abrupt hair loss in diffuse alopecia areata (AA remains unclear. Aims: To explore the characteristics of diffuse AA and possible factors involved in its pathogenesis. Methods: Clinical and laboratory data of 17 diffuse AA patients and 37 patchy AA patients were analyzed retrospectively. Serum IgE level was evaluated in all diffuse and patchy AA patients, as well as 27 healthy subjects without hair loss to serve as normal control. Univariate analysis was performed using Fisher′s exact test and Wilcoxon rank-sum test. Associations between inflammatory cell infiltration and laboratory values were analyzed using Spearman rank correlation test. Results: The mean age of patients with diffuse AA was 27 years with a mean disease duration of 1.77 months. All of them presented in spring or summer with an acute onset of diffuse hair loss preceded by higher incidence of scalp pruritus. Although no statistically significant difference on the incidence of atopic disease among three groups has been found, serum IgE level in diffuse AA was higher than that in healthy controls, but was comparable to that in patchy AA group. Histopathology of lesional scalp biopsies showed more intense infiltration comprising of mononuclear cells, eosinophils, CD3 + , and CD8 + T cells around hair bulbs in diffuse AA group than in patchy AA group. Moreover, IgE level in diffuse AA patients positively correlated with intensity of infiltration by mononuclear cells, eosinophils, and CD8 + T cells. Conclusions: Hypersensitivity may be involved in pathogenesis of diffuse AA. The acute onset of diffuse AA may be related to intense local inflammatory infiltration of hair loss region and an increase in serum IgE level.

  6. Diagnostic value of scratch-chamber test, skin prick test, histamine release and specific IgE in birch-allergic patients with oral allergy syndrome to apple

    DEFF Research Database (Denmark)

    Osterballe, M; Scheller, R; Stahl Skov, P

    2003-01-01

    BACKGROUND: The aim of the study was to examine the diagnostic value of skin prick test (SPT), scratch-chamber test (SCT), histamine release (HR) and specific immunoglobulin E (IgE) in birch-allergic patients with oral allergy syndrome to apple. METHODS: Ten birch-allergic patients with oral...... a detailed case history about symptoms of oral allergy syndrome combined with a SPT with fresh apple peel or A72 will be useful....

  7. Distribución municipal de la incidencia de los tumores más frecuentes en un área de elevada mortalidad por cáncer

    Directory of Open Access Journals (Sweden)

    Manuel Jesús Viñas Casasola

    2017-03-01

    Conclusiones: En la provincia de Huelva existe una distribución espacial municipal de la incidencia de cáncer con unos patrones bien definidos para algunas localizaciones tumorales concretas, presentando en general unas tasas de incidencia cercanas a las del territorio nacional.

  8. Recubrimiento de acero con polidopamina

    OpenAIRE

    Carrasco Rodríguez, Javier

    2013-01-01

    Se ha obtenido recubrimientos de polidopamina en acero mecánicamente resistentes y con tiempos de obtención relativamente pequeños a través de la polimerización de la dopamina bajo diferentes condiciones.

  9. Mayor frecuencia de giardiosis en niños con raquitismo comparada con niños sanos de la población de Suárez, Cauca, Colombia

    Directory of Open Access Journals (Sweden)

    Oscar L. Sierra

    1998-12-01

    Full Text Available Se examinaron huevos, quistes y trofozoítos de parásitos intestinales en heces de 332 niños sanos y 70 niños con raquitismo descrito como un trastorno heredado debido a malabsorción de calcio por resistencia a la vitamina D en Suárez, departamento del Cauca. La frecuencia total de parasitosis fue de 65,9% y la de poliparasitismo de 10,9%. Se encontraron frecuencias elevadas de giardiosis y poliparasitismo en niños con raquitismo (17,l y 18,6% comparado con niños sanos (9,O y 9,3% y dichas diferencias fueron estadísticamente significativas. La vereda de La Toma, en donde se ha hallado el mayor número de raquíticos, pareció conferir un riesgo incrementado para ascariosis, pero, independiente del raquitismo. Se discuten algunos mecanismos para explicar la mayor frecuencia de parasitosis en los niños raquíticos. La incrementada frecuencia de parasitosis intestinales patógenas puede contribuir a disminuir la absorción intestinal de calcio en los niños raquíticos.

  10. Combined effect of glycation and sodium carbonate-bicarbonate buffer concentration on IgG binding, IgE binding and conformation of ovalbumin.

    Science.gov (United States)

    Ma, Xiao-juan; Gao, Jin-yan; Chen, Hong-bing

    2013-10-01

    Ovalbumin (OVA) is a major allergen in hen egg. During thermal processing, reducing sugars contained in the hen egg white might easily undergo glycation with OVA, but few studies have been conducted on its corresponding immunoreactivity changes. The aim of the present study was to assess changes of the antigenicity, potential allergenicity and conformation of OVA after glycation in a wet-thermal processing system under different concentrations of sodium carbonate-bicarbonate buffer. IgE binding of the glycated OVA was increased after glycation, and the higher the sodium carbonate-bicarbonate buffer concentration, the higher the IgE binding capacity. The increase in IgE binding of OVA corresponded well with the disruption of the disulfide bond, which exposed the epitopes initially buried. Antigenicity of the glycated OVA was increased, and the amount of the increase varied among samples treated under different buffer concentrations. Glycation increased the allergenic potential for OVA, with the amount of increase varying with different sodium carbonate-bicarbonate buffer concentrations. © 2013 Society of Chemical Industry.

  11. Mutational epitope analysis of Pru av 1 and Api g 1, the major allergens of cherry (Prunus avium) and celery (Apium graveolens): correlating IgE reactivity with three-dimensional structure.

    Science.gov (United States)

    Neudecker, Philipp; Lehmann, Katrin; Nerkamp, Jörg; Haase, Tanja; Wangorsch, Andrea; Fötisch, Kay; Hoffmann, Silke; Rösch, Paul; Vieths, Stefan; Scheurer, Stephan

    2003-01-01

    Birch pollinosis is often accompanied by adverse reactions to food due to pollen-allergen specific IgE cross-reacting with homologous food allergens. The tertiary structure of Pru av 1, the major cherry (Prunus avium) allergen, for example, is nearly identical with Bet v 1, the major birch (Betula verrucosa) pollen allergen. In order to define cross-reactive IgE epitopes, we generated and analysed mutants of Pru av 1 and Api g 1.0101, the major celery (Apium graveolens) allergen, by immunoblotting, EAST (enzyme allergosorbent test), CD and NMR spectroscopy. The mutation of Glu45 to Trp45 in the P-loop region, a known IgE epitope of Bet v 1, significantly reduced IgE binding to Pru av 1 in a subgroup of cherry-allergic patients. The backbone conformation of Pru av 1 wild-type is conserved in the three-dimensional structure of Pru av 1 Trp45, demonstrating that the side chain of Glu45 is involved in a cross-reactive IgE epitope. Accordingly, for a subgroup of celery-allergic patients, IgE binding to the homologous celery allergen Api g 1.0101 was enhanced by the mutation of Lys44 to Glu. The almost complete loss of IgE reactivity to the Pru av 1 Pro112 mutant is due to disruption of its tertiary structure. Neither the mutation Ala112 nor deletion of the C-terminal residues 155-159 influenced IgE binding to Pru av 1. In conclusion, the structure of the P-loop partially explains the cross-reactivity pattern, and modulation of IgE-binding by site-directed mutagenesis is a promising approach to develop hypo-allergenic variants for patient-tailored specific immunotherapy. PMID:12943529

  12. Características de la función inmune celular en pacientes con alergia o intolerancia a las proteínas de la leche de vaca : estudio comparativo

    OpenAIRE

    Samartín Sáenz, Sonia

    2003-01-01

    Con el fin de contribuir a un mejor conocimiento del mecanismos biológico de la alergia y de la intolerancia a las proteínas de la leche de vaca, se evaluó un grupo de niños con sospecha de alergia a la leche. Se determinaron en todos los niños los parámetros antropométricos, bioquímicos, hematológicos, así como los inmunológicos para evaluar la presencia de IgE y de un posible mecanismo Inmunológico mediado por células. Se llevaron también a cabo pruebas de provocación oral con la fórmula lá...

  13. The 11S globulin Sin a 2 from yellow mustard seeds shows IgE cross-reactivity with homologous counterparts from tree nuts and peanut

    Directory of Open Access Journals (Sweden)

    Sirvent Sofía

    2012-12-01

    Full Text Available Abstract Background The 11S globulin Sin a 2 is a marker to predict severity of symptoms in mustard allergic patients. The potential implication of Sin a 2 in cross-reactivity with tree nuts and peanut has not been investigated so far. In this work, we studied at the IgG and IgE level the involvement of the 11S globulin Sin a 2 in cross-reactivity among mustard, tree nuts and peanut. Methods Eleven well-characterized mustard-allergic patients sensitized to Sin a 2 were included in the study. A specific anti-Sin a 2 serum was obtained in rabbit. Skin prick tests (SPT, enzyme-linked immunosorbent assay (ELISA, immunoblotting and IgG or IgE-inhibition immunoblotting experiments using purified Sin a 2, Sin a 1, Sin a 3, mustard, almond, hazelnut, pistachio, walnut or peanut extracts were performed. Results The rabbit anti-Sin a 2 serum showed high affinity and specificity to Sin a 2, which allowed us to demonstrate that Sin a 2 shares IgG epitopes with allergenic 11S globulins from tree nuts (almond, hazelnut, pistachio and walnut but not from peanut. All the patients included in the study had positive skin prick test to tree nuts and/or peanut and we subdivided them into two different groups according to their clinical symptoms after ingestion of such allergenic sources. We showed that 11S globulins contain conserved IgE epitopes involved in cross-reactivity among mustard, tree nuts and peanut as well as species-specific IgE epitopes. Conclusions The allergenic 11S globulin Sin a 2 from mustard is involved in cross-reactivity at the IgE level with tree nuts and peanut. Although the clinical relevance of the cross-reactive IgE epitopes present in 11S globulins needs to be investigated in further detail, our results contribute to improve the diagnosis and management of mustard allergic patients sensitized to Sin a 2.

  14. Santiago, una ciudad con temor

    Directory of Open Access Journals (Sweden)

    Enrique Oviedo S.

    1999-04-01

    Full Text Available El objetivo general de este artículo es evaluar los efectos de la inseguridad ciudadana en el uso del espacio público. Dicha evaluación exige analizar dos relaciones que se establecen en el ámbito de la violencia: la relación entre victimización y percepción de inseguridad; y la que se establece entre actitudes sociales y resolución pacífica de conflictos nacionales. Para ello, se analizaron las variables victimización, percepción de inseguridad, uso del espacio físico, actitudes hacia el sistema institucional político y social y hacia la resolución de conflictos nacionales, y las posibles relaciones entre ellas. Los datos para realizar el estudio se obtuvieron por medio de una encuesta que se llevó a cabo con 1 200 personas de 18 y 70 años de edad residentes en la ciudad de Santiago. Los resultados indican que Santiago es una ciudad de habitantes con temor y que el aumento de la percepción de inseguridad de sus habitantes contrasta con el hecho de que las tasas de victimización se hayan mantenido, más o menos, constantes en los años que precedieron a la encuesta. El temor se relaciona con el abandono del espacio público físico y sociopolítico, así como con el refugio en los espacios y la vida privados. La actitud de resolver los conflictos por medios no pacíficos es frecuente y se asocia en mayor medida con la inseguridad, la actitud negativa hacia la democracia y la falta de expectativas sobre el futuro del país. Los resultados de este estudio respaldan la idea de que para superar el temor la gente tiende a adaptarse a la realidad adoptando una postura conformista, homogeneizando las creencias y los comportamientos, y sobreestimando la fuerza como medio para resolver las diferencias.

  15. Santiago, una ciudad con temor

    Directory of Open Access Journals (Sweden)

    Oviedo S. Enrique

    1999-01-01

    Full Text Available El objetivo general de este artículo es evaluar los efectos de la inseguridad ciudadana en el uso del espacio público. Dicha evaluación exige analizar dos relaciones que se establecen en el ámbito de la violencia: la relación entre victimización y percepción de inseguridad; y la que se establece entre actitudes sociales y resolución pacífica de conflictos nacionales. Para ello, se analizaron las variables victimización, percepción de inseguridad, uso del espacio físico, actitudes hacia el sistema institucional político y social y hacia la resolución de conflictos nacionales, y las posibles relaciones entre ellas. Los datos para realizar el estudio se obtuvieron por medio de una encuesta que se llevó a cabo con 1 200 personas de 18 y 70 años de edad residentes en la ciudad de Santiago. Los resultados indican que Santiago es una ciudad de habitantes con temor y que el aumento de la percepción de inseguridad de sus habitantes contrasta con el hecho de que las tasas de victimización se hayan mantenido, más o menos, constantes en los años que precedieron a la encuesta. El temor se relaciona con el abandono del espacio público físico y sociopolítico, así como con el refugio en los espacios y la vida privados. La actitud de resolver los conflictos por medios no pacíficos es frecuente y se asocia en mayor medida con la inseguridad, la actitud negativa hacia la democracia y la falta de expectativas sobre el futuro del país. Los resultados de este estudio respaldan la idea de que para superar el temor la gente tiende a adaptarse a la realidad adoptando una postura conformista, homogeneizando las creencias y los comportamientos, y sobreestimando la fuerza como medio para resolver las diferencias.

  16. CONCEPCIONES DE LOS DOCENTES NO UNIVERSITARIOS SOBRE EL APRENDIZAJE COLABORATIVO CON TIC

    Directory of Open Access Journals (Sweden)

    Azucena Hernández Martín

    2017-01-01

    Full Text Available Actualmente estamos ante una situación de importantes cambios, tanto en lo que concierne al ámbito universitario, con la implantación del Espacio Europeo de Educación Superior, propiciando que los docentes nos replanteemos metodologías de carácter convencional; como en lo que atañe a niveles educativos no universitarios, en donde la progresiva dotación de aulas digitales está generando paulatinamente un cambio de cultura de las instituciones educativas y de sus protagonistas: docentes y estudiantes. Las herramientas de la Web 2.0 están siendo empleadas en el contexto de enfoques metodológicos con un matiz claramente colaborativo. Todo ello está generando que estas metodologías de aprendizaje colaborativo a través de las TIC, independientemente del nivel educativo, tengan una importante proyección en procesos de innovación educativa. El artículo presenta las concepciones que el profesorado no universitario tiene sobre dicha metodología, incidiendo en la valoración y expectativas de la misma para el desarrollo del currículo. Así mismo se analizan los conocimientos que poseen sobre herramientas y aplicaciones de trabajo colaborativo con TIC. Dichas concepciones y conocimientos han sido recogidos mediante un cuestionario on-line, permitiéndonos conocer cómo actualmente las expectativas sobre las TIC para impulsar este tipo de aprendizaje son elevadas, si bien se carece aún de la suficiente capacitación para que dichas percepciones se materialicen en cambios reales en las prácticas cotidianas de centro y aula.

  17. Antígeno carcinoembrionario en la recurrencia y sobrevida de pacientes con resección curativa de cáncer colorrectal

    Directory of Open Access Journals (Sweden)

    Jorge Renato Cribilleros Barrenechea

    2014-04-01

    Full Text Available Introducción: El antígeno carcinoembrionario (CEA ha sido asociado con estadios avanzados, pobre sobrevida y detección temprana de recurrencia de cáncer colorrectal (CCR. Objetivos: Establecer la relación entre la concentración sérica de CEA con la recurrencia y sobrevida de pacientes con resección curativa de cáncer colorrectal. Diseño: Estudio retrospectivo, correlacional y explicativo. Institución: Departamento de Cirugía General, Servicio de Colon y Recto, Hospital Nacional Edgardo Rebagliati Martins, EsSalud, Lima, Perú. Población: Pacientes con resección curativa de CCR. Métodos: Los pacientes con resección curativa de CCR en el hospital Rebagliati, durante los años 2000-2003, tuvieron seguimiento hasta el año 2010. Se usó la diferencia de medias a través de la prueba T, para la comparación de variables cuantitativas. Se recurrió a técnicas de análisis de sobrevida a través del método de Kaplan-Meier y la regresión de Cox. Principales medidas de resultados: Recurrencia y sobrevida de pacientes. Resultados: La concentración sérica elevada de antígeno carcinoembrionario en el pre y postoperatorio reveló una mayor recurrencia significativa de cáncer colorrectal (p<0,05. La sobrevida en los pacientes con CEA patológico fue significativamente menor que en los pacientes con CEA normal (p<0,05. Conclusiones: La concentración sérica elevada de CEA, demostró una mayor recurrencia del cáncer colorrectal. El valor sérico de CEA en el preoperatorio constituye un valor predictivo de sobrevida.

  18. Characteristics of the Biological Effects and the RBE of High Energy Protons; Caracteristiques des Effets Biologiques et EBR des Protons de Haute Energie; Osobennosti biologicheskogo dejstviya i obeh protonov vysokikh ehnergii; Caracteristicas de los Efectos Biologicos y de la EBR de los Protones de Elevada Energia

    Energy Technology Data Exchange (ETDEWEB)

    Grigor' ev, Ju. G.; Darenskaja, N. G.; Domshlak, M. M.; Lebedinskij, A. V.; Nefedov, Ju. G.; Ryzhov, N. I.

    1964-03-15

    The characteristics of the biological effects of high-energy protons (120, 240 and 510 MeV) were studied in experiments on mice, rats and dogs. It was shown that together with a certain resemblance or identity in radiation damage due to the effects of X-rays and protons, there were certain differences in the case of proton irradiation. In the proton irradiation of dogs the haemorrhagic syndrome was more pronounced. Haemorrhage appeared earlier in the animals and was more abundant. A difference was found in proton RBE levels for small animals (rats, mice) and large animals (dogs). This difference is quite large and equals respectively 0.7 and 1.0 * 1.15. Some considerations are presented in the report on the cause of these differences. (author) [French] Les auteurs ont etudie les caracteristiques des effets biologiques des protons de haute energie (120, 240 et 510 MeV) au cours d'experiences sur des souris, des rats et des chiens. Ils montrent que si les dommages causes par les rayons X et les protons presentent une certaine ressemblance ou des caracteres identiques, on observe des differences dans le cas de l'irradiation par les protons. Chez les chiens exposes aux protons, le syndrome hemorragique etait plus prononce. L'hemorragie s'est manifestee plus tot chez ces animaux et etait plus abondante. Les auteurs ont decele une difference dans l*EBRdes protons pour les petits animaux (rats, souris) et pour les grands animaux (chiens). Cette difference etait importante: 0,7 dans le premier cas et de 1,0 a 1,15 dans le second cas. On trouve dans le memoire quelques considerations sur la cause de ces differences. (author) [Spanish] Los autores estudiaron las caracteristicas de los efectos biologicos de los protones de elevada energia (120, 240 y 510 MeV) mediante experimentos con ratones, ratas y perros. Comprobaron que a pesar de ciertas semejanzas en las radiolesiones causadas por los rayos X y los protones, los efectos de estos ultimos acusan algunas diferencias. El

  19. Factores clínicos relacionados con la hipertensión arterial en pacientes con trastornos de tolerancia a los carbohidratos Clinical factors related to arterial hypertension in patients with impaired carbohydrate tolerance

    Directory of Open Access Journals (Sweden)

    Marelys Yanes Quesada

    2007-12-01

    Full Text Available La hipertensión arterial constituye una de las enfermedades más frecuentes en el momento actual. Su asociación con trastornos del metabolismo de la glucosa, acelera el grado de intolerancia y de sus complicaciones. Con el objetivo de identificar algunos factores clínicos asociados a ella en pacientes con trastornos de la tolerancia a la glucosa, se realizó un estudio descriptivo, transversal, con 329 pacientes con antecedentes de una hiperglucemia previa, a los que se les realizó una prueba de tolerancia a la glucosa para medir glucemia. Se les recogieron los datos siguientes: edad, sexo, peso, talla, se calculó el índice de masa corporal y tensión arterial. Se encontró una asociación significativa de la hipertensión arterial con el incremento de la edad, el sexo masculino y un mayor índice de masa corporal. La frecuencia de hipertensión arterial fue de un 59,9 %, y se incrementó hasta un 76,2 % en los pacientes con mayor número de factores de riesgo. La hipertensión arterial tiene una elevada prevalencia en pacientes con trastornos de tolerancia a la glucosa, la edad avanzada, el sexo masculino y el índice de masa corporal elevado, que a su vez se relacionan con su incremento, y cuando los sujetos presentan varios factores de riesgo asciende la frecuencia de hipertensión arterial.Arterial hypertension is one of the most common diseases at present. Its association with glucose metabolism disorders speeds up the intolerance degree and its complications. In order to identify some clinical factors associated with it in patients with impaired glucose tolerance, a descriptive cross-sectional study was conducted among 329 patients with previous history of hyperglycemia. A glucose tolerance test was performed to measure glycemia. The following data were collected: age, sex, weight and height. The body mass index and the arterial hypertension were calculated. The arterial hypertension frequency had an elevated prevalence of patients

  20. pacientes con insuficiencia renal terminal

    Directory of Open Access Journals (Sweden)

    Karen Herrera Herrera

    2011-01-01

    Full Text Available La presente investigación fundamenta en la clínica psicoanalítica el estudio de dos casos de tres personas diagnosticadas con IRT que reciben tratamiento de hemodiálisis, en razón a que dadas las características y el aumento de los reportes que se presentan, ya esto se considera un problema de salud pública. El objetivo principal es describir las características dinámicas del proceso de duelo en pacientes con IRT en un centro de terapia renal de la ciudad de Cartagena. El procedimiento metodológico empleó un diseño de tipo cualitativo; la investigación se desarrolló con un diseño clínico mediante el estudio de casos, y fundamentada en la hermenéutica psicoanalítica. Todo esto respaldado en la historia clínica, la entrevista semiestructurada individual y familiar, los test proyectivos, test del dibujo de la figura humana Machover y TAT de Murray, para la debida integración de los análisis. Se concluye que predominan funciones fallidas de los progenitores y que son individuos provenientes de familias psicosomáticas, que utilizan la enfermedad para obtener un beneficio secundario.

  1. con bajo peso al nacer

    Directory of Open Access Journals (Sweden)

    Adriana Mora Antó

    2005-01-01

    Full Text Available Esta investigación dio cuenta de la relación entre el estilo de funcionamiento familiar, los patrones de crianza y las edades de desarrollo evolutivo en niños, nacidos con bajo peso. El estudio descriptivo correlacional se realizó con 41 niños y sus madres, aplicándose cuestionarios sobre funcionamiento familiar, prácticas de crianza y desarrollo infantil. Los resultados señalaron la existencia de un funcionamiento familiar caracterizado por una cohesión amalgamada y una adaptabilidad caótica, una disciplina complaciente, falta de control y de límites claros en la díada madre-hijo. Se trataba de familias monoparentales, donde la temprana edad de concepción, el madresolterismo y el apoyo de la familia extensa eran constantes. Las edades evolutivas registradas indicaron un desarrollo inferior a la edad cronológica, en la mayor parte de los casos; sin embargo, éstas tendieron a ser superiores al compararlas con la edades reales de los infantes. No se encontró una correlación estadísticamente significativa entre la edad de desarrollo y los diferentes factores del funcionamiento familiar para algunos de los rangos de edad considerados; sin embargo, no se lo descartó por completo, especialmente en lo referente al optimismo familiar

  2. The Impact of Baked Egg and Baked Milk Diets on IgE- and Non-IgE-Mediated Allergy.

    Science.gov (United States)

    Upton, Julia; Nowak-Wegrzyn, Anna

    2018-03-08

    Baked milk (BM) and baked egg (BE) diets are increasingly used in the management of milk and egg allergy, rather than avoidance. Children with tolerance versus reactivity to BM and BE may have smaller skin prick test and lower specific IgE, and BM-tolerant children have less basophil reactivity and more peripheral T regulatory cells. However, most milk- and egg-allergic children tolerate BM and BE and an individual's reactivity is unpredictable. Non-reactivity is due to conformational changes in the allergens. Significant differences in the published advice about methods of introduction exist from graded introduction at home to a medically supervised full dose. These approaches carry different risks and may have different immunological effects. Reactivity to BM is a predictor of a severe milk allergy. Therefore, medical supervision for BM and BE introduction is prudent. The baked diet allows dietary liberation. Most, but not all, BM- and BE-tolerant children continue eating the baked foods. The prognosis of children who can eat BM and BE is favorable with likely resolution of their allergy over the next few years. Murine models of BE diets demonstrate that heated egg can impart clinical protection against anaphylaxis and cause immune changes. Most observational human studies of BM and BE diets demonstrate clinical resolution of allergy and favorable immune changes versus regular care controls. However, the one randomized controlled trial for the BE diet in BE-tolerant children did not support an immune-modifying effect of the BE diet. Another study of BE immunotherapy is expected to be completed in 2018. There is currently no evidence for prevention of allergy with the baked diets. There may be a future role for BM and BE in liberating the diets of individuals with non-IgE-mediated allergy given recent studies that a subset of these patients can consume BM without a clinical reaction.

  3. Exhaled nitric oxide levels in school children in relation to IgE sensitisation and window pane condensation.

    Science.gov (United States)

    Janson, Christer; Kalm-Stephens, Pia; Foucard, Tony; Norbäck, Dan; Alving, Kjell; Nordvall, S Lennart

    2005-08-01

    A positive relation between exhaled nitric oxide (NO) levels and allergen exposure has been found in some studies whereas there is less information on how non-allergen environmental factors influences exhaled NO. To study the relationship between exhaled NO levels in schoolchildren in relation to IgE sensitisation and allergenic and non-allergenic environmental factors. This study comprised 374 schoolchildren (13-14 years of age) who performed exhaled NO-measurements and skin prick tests. Exposure to allergens, respiratory infections, environmental tobacco smoke and home window pane condensation, the latter an indicator of high humidity and poor ventilation was evaluated through questionnaires. In IgE-sensitised children sensitisation to pets was a more important determinant of exhaled NO than sensitisation to pollen. Higher NO levels were found in cat-sensitised children with a cat or other furred pets at home compared to cat-sensitised children without pets (geometric mean, 24.0 vs. 13.9 ppb, P=0.03). Significantly higher exhaled NO levels were found in non-sensitised children that reported having a cold (5.7 vs. 3.8 ppb, P<0.001) or lived in homes with window pane condensation (7.1 vs. 4.4 ppb, P=0.01) than in non-sensitised children without a cold and window pane condensation, respectively. These associations were not found in children that were sensitised to inhalation allergens. Allergen exposure seems to be the most important determinant for exhaled NO levels in IgE-sensitised children whereas in non-sensitised children NO levels were associated with respiratory infections and home window pane condensation.

  4. Peanut sensitization pattern in Norwegian children and adults with specific IgE to peanut show age related differences.

    Science.gov (United States)

    Namork, Ellen; Stensby, Berit A

    2015-01-01

    Peanuts contain potent food allergens and the prevalence of allergy is reported to increase, especially in children. Since peanut sensitization may differ between different geographical regions, we wanted to investigate the sensitization pattern to the individual peanut allergens in a Norwegian population. Cases reported to the Norwegian Food Allergy Register with sera positive to peanut extract were analyzed for specific IgE (sIgE) to the recombinant peanut allergens Ara h 1, Ara h 2, Ara h 3, Ara h 8 and Ara h 9 and to birch pollen extract. Serum samples negative to the above allergens were analyzed for sIgE to Ara h 6, and sIgE to Pru p 3 in peach were analyzed in sera positive to the cross-reactive allergen Ara h 9. Highest frequency of sIgE to Ara h 2, often co-sensitized to Ara h 1 and 3, were found in the small children up to 6 years of age. From the age of 6 years, sensitization to Ara h 8 was predominant. The sIgE levels to the storage proteins Ara h 1, 2 and 3 were strongly correlated, as was the sIgE levels to Ara h 8 and birch pollen extract. A low sensitization rate of sIgE to Ara h 9 in young adults was observed, which sIgE levels were very strongly correlated to Pru p 3. The sensitization to peanut allergens in a Norwegian population shows a clear age dependent pattern. The results add to the previously published research on the sensitization patterns of peanut sensitized patients in different geographical areas.

  5. Specific IgE for Fag e 3 Predicts Oral Buckwheat Food Challenge Test Results and Anaphylaxis: A Pilot Study.

    Science.gov (United States)

    Yanagida, Noriyuki; Sato, Sakura; Maruyama, Nobuyuki; Takahashi, Kyohei; Nagakura, Ken-Ichi; Ogura, Kiyotake; Asaumi, Tomoyuki; Ebisawa, Motohiro

    2018-01-01

    Buckwheat (BW) is the source of a life-threatening allergen. Fag e 3-specific serum IgE (sIgE) is more useful than BW-sIgE for diagnosis; however, it is unknown whether Fag e 3-sIgE can predict oral food challenge (OFC) results and anaphylaxis. This study aimed to clarify the efficacy of Fag e 3-sIgE in predicting OFC results and anaphylaxis. We conducted a retrospective review of BW- and Fag e 3-sIgE data obtained using the ImmunoCAP® assay system and fluorescent enzyme-linked immunosorbent assay from children who underwent OFC using 3,072 mg of BW protein between July 2006 and March 2014 at Sagamihara National Hospital, Kanagawa, Japan. We analyzed 60 patients aged 1.9-13.4 years (median 6.0 years); 20 (33%) showed objective symptoms upon BW OFC. The patients without symptoms had significantly lower Fag e 3-sIgE than those with non-anaphylactic (p tested factor that significantly predicted positive OFC results (odds ratio 8.93, 95% confidence interval 3.10-25.73, p < 0.001) and OFC-induced anaphylaxis (2.67, 1.12-6.35, p = 0.027). We suggest that a threshold Fag e 3-sIgE level of 18.0 kUE/L has 95% probability of provoking a positive reaction to BW. Fag e 3-sIgE predicted OFC results and OFC-induced anaphylaxis. We further emphasize paying careful attention to the risk of BW OFC-induced anaphylaxis. © 2018 The Author(s) Published by S. Karger AG, Basel.

  6. ImmunoCAP assays: Pros and cons in allergology.

    Science.gov (United States)

    van Hage, Marianne; Hamsten, Carl; Valenta, Rudolf

    2017-10-01

    Allergen-specific IgE measurements and the clinical history are the cornerstones of allergy diagnosis. During the past decades, both characterization and standardization of allergen extracts and assay technology have improved. Here we discuss the uses, advantages, misinterpretations, and limitations of ImmunoCAP IgE assays (Thermo Fisher Scientific/Phadia, Uppsala, Sweden) in the field of allergology. They can be performed as singleplex (ImmunoCAP) and, for the last decade, as multiplex (Immuno Solid-phase Allergen Chip [ISAC]). The major benefit of ImmunoCAP is the obtained quantified allergen-specific IgE antibody level and the lack of interference from allergen-specific IgG antibodies. However, ImmunoCAP allergen extracts are limited to the composition of the extract. The introduction of allergen molecules has had a major effect on analytic specificity and allergy diagnosis. They are used in both singleplex ImmunoCAP and multiplex ImmunoCAP ISAC assays. The major advantage of ISAC is the comprehensive IgE pattern obtained with a minute amount of serum. The shortcomings are its semiquantitative measurements, lower linear range, and cost per assay. With respect to assay performance, ImmunoCAP allergen extracts are good screening tools, but allergen molecules dissect the IgE response on a molecular level and put allergy research on the map of precision medicine. Copyright © 2017 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  7. Regulation of IgE antibody production by serum molecules. II. Strain-specificity of the suppressive activity of serum from complete Freund's adjuvant-immune low responder mouse donors

    International Nuclear Information System (INIS)

    Katz, D.H.; Tung, A.S.

    1978-01-01

    IgE antibody production in mice of high and low IgE responder phenotypes, respectively, can be appreciably enhanced in magnitude after low-dose whole-body x irradiation. Such enhanced responses, as well as adoptive secondary IgE responses, can be markedly suppressed by passive transfer of CFA-immune serum in low responder strains, but not in high responder strains. The studies presented here demonstrate that the suppressive activity of CFA-immune serum on IgE antibody production is strain specific. This is true even in reciprocal combinations of low IgE responder SJL and C57BL/6 mice, in which it was shown that serum capable of suppressing mice of the isologous strain was ineffective in diminishing IgE antibody production in the other low responder strain. Absence of suppressive activity in CFA-immune sera obtained from H-2 haplotypes while sharing many similarities in the background genome and, conversely, effective suppressive activity of H-2 congenic donor sera when H-2-identities between donor and recipient mice existed, strongly suggested a role, at least in part, of H-2 genes in dictating the strain specificity of such suppressive activity. Additional experiments provided evidence for a possible role of macrophages in catabolism of the active molecules in CFA-immune sera. These observations, together with those presented in the preceding paper, may provide valuable insight toward successful development of appropriate manipulations that could ultimately convert high IgE responder individuals into low responders

  8. The luminescent chamber and its use in high-energy physics experiments; La chambre a luminescence: son emploi dans les experiences de physique des hautes energies; Lyuminestsentnaya kamera i ee ispol'zovanie v ehksperimentakh, provodimykh v fizike vysokikh ehnergij; La camara luminiscente y su empleo en experimentos de fisica de elevada energia

    Energy Technology Data Exchange (ETDEWEB)

    Jones, L W; Perl, M L [Randall Laboratory of Physics, University of Michigan, Ann Arbor, MI (United States); Lawrence Radiation Laboratory, University of California, Berkeley, CA (United States)

    1962-04-15

    electronique du compteur a scintillations. Les auteurs exposent les travaux de preparation et d'execution qui ont ete necessaires pour mener ces experiences a bonne fin; ils donnent, en particulier, les details voulus sur les tubes a rayons cathodiques, les compteurs a scintillations et les systemes electroniques utilises. L'analyse de photographies de trajectoires leur a permis d'etudier, notamment, le degre de precision qui peut etre atteint et la valeur des resultats physiques qui peuvent etre obtenus. Enfin, ils explorent les possibilites de developpement qui s'offrent a la chambre a luminescence, compte tenu des tubes renforcateurs d'image et des scintillateurs actuellement a l'etude. Ils analysent le role que la chambre a luminescence est appelee a jouer a l'avenir dans les experiences du domaine des grandes energies en comparaison avec la chambre a etincelles et les autres detecteurs. (author) [Spanish] Por primera vez se ha empleado una camara luminescente en experimentos de fisica con particulas de elevada energia. En el Bevatron del Lawrence Radiation Laboratory se fotografiaron trazas de particulas en cristales de yoduro sodico activado por medio de un sistema en cascada de tres tubos intensificadores de imagen. En un experimento se estudio la dispersion elastica pion-proton utilizando dos conjuntos de centelladores, cada uno de 20 cm de longitud. Uno para observar el pion dispersado y, el otro, el proton de retroceso procedente de un blanco de hidrogeno liquido. Los contadores de centelleo seleccionaban unicamente los sucesos casi coplanares que habian de ser registrados; no obstante, respecto de la dispersion inelastica, el criterio definitivo era la cinematica de las trazas registradas. Se fotografiaron alrededor de 1000 sucesos elasticos correspondientes cada uno a tres cantidades de movimiento del pion incidente hasta 2,5 MeV/c. En un segundo experimento se utilizo unicamente un conjunto de centelleadores de 10 x 10 x 20 cm{sup 3} para observar el frenado de

  9. Propiedades psicométricas de la Escala de Locus de Control de la Salud en Dios en una muestra de mexicanos con diabetes mellitus tipo 2

    Directory of Open Access Journals (Sweden)

    Vianey Guadalupe Argüelles Nava

    2017-12-01

    Full Text Available En este estudio se obtuvo evidencia de validez y confiabilidad de la escala de Locus de Control de la Salud en Dios (GLHC en una muestra de 539 adultos mexicanos con diabetes mellitus tipo 2. El análisis de validez se basó en el análisis del contenido de los ítems y de la estructura interna del GLHC. El primer procedimiento estuvo a cargo de seis expertos, quienes evaluaron la pertinencia de la adaptación de los ítems. El segundo procedimiento fue un análisis factorial confirmatorio de grupo múltiple, de acuerdo al sexo y edad, a fin de evaluar la invarianza de medición del GLHC. Finalmente, la confiabilidad fue calculada mediante los coeficientes ω y H. El análisis factorial confirmatorio demostró una estructura de un solo factor con seis ítems, interpretable para la población de estudio con invarianza de medición por sexo, aunque deben tomarse precauciones al interpretar comparaciones por grupos de edad. La confiabilidad del constructo obtuvo magnitudes elevadas (>.90. Los resultados indican que la GLHC puede brindar información válida y confiable para valorar el locus de control de la salud en Dios para mexicanos con diabetes mellitus tipo 2.

  10. Evolución clínica de pacientes asmáticos tratados con inmunoterapia. Municipio Majibacoa, 2008-2013

    Directory of Open Access Journals (Sweden)

    Mailin Molina Leyva

    2015-11-01

    Full Text Available Se realizó un estudio cuasi-experimental de corte longitudinal en 50 pacientes asmáticos con edades  entre 5 y 54 años, atendidos en el Servicio de Alergología del municipio Majibacoa de la provincia de Las Tunas; con el objetivo de determinar la evolución clínica con inmunoterapia, en el período de enero 2008 a febrero de 2013. A los pacientes se les administró inmunoterapia específica de ácaros con extractos estandarizados, durante tres años o más de forma subcutánea. El asma bronquial predominó en el sexo femenino y en las edades comprendidas entre 5 y 14 años. Los antecedentes de atopia familiar fueron referidos por el mayor por ciento de los pacientes analizados. Según severidad de la enfermedad, antes del tratamiento prevalecieron los pacientes en la categoría persistente moderada. Se observó mejoría de los síntomas del asma en una elevada proporción de casos.

  11. Evaluación del peso al nacer en 92 niños con fisuras del labio y del paladar

    Directory of Open Access Journals (Sweden)

    Carlos Evaristo Zamora Linares

    Full Text Available Objetivo: describir y evaluar algunos aspectos relacionados con el peso al nacer en una población de niños con fisuras de labio y paladar. Métodos: estudio descriptivo con fase analítica realizado en el Hospital Pediátrico Provincial "Hermanos Cordové", de Manzanillo, Cuba. Se incluyeron, luego de ser seleccionados por conveniencia, 92 niños nacidos con fisuras bucales que fueron atendidos en el centro entre los años 1986 y 2010. Se consideraron las variables siguientes: sexo, peso al nacer y tipo de fisura. Mediante medidas descriptivas se establecen asociaciones intervariables. Los aspectos analíticos incluyen el análisis de varianza y la estimación de riesgo (odds ratio y su intervalo de confianza del 95 %. Resultados: la prevalencia de bajo peso al nacer en estos pacientes (14,1 % resultó elevada, en comparación con la población general. Hubo una asociación significativa entre el tipo de fisura y el peso al nacer (X²= 12,65. El riesgo de bajo peso al nacer en los pacientes con fisuras labiales asociadas a fisuras palatinas fue significativo (RP= 6,24; IC 95 %: 1,3-30. Conclusiones: los resultados del estudio se corresponden de manera general con los obtenidos por otros autores. Aparentemente hay una asociación entre el nivel de gravedad de las fisuras y el riesgo de bajo peso al nacer.

  12. Asociación entre el precio de los alimentos y la glucemia en adultos estadounidenses con diabetes de tipo 2

    Directory of Open Access Journals (Sweden)

    Tobenna D. Anekwé

    2015-06-01

    Full Text Available OBJETIVOS: Calculamos la asociación entre el precio de diversas categorías de alimentos saludables y menos saludables y la glucemia en adultos estadounidenses con diabetes de tipo 2. MÉTODOS: Vinculamos la información de salud contenida en la Encuesta Nacional de Salud y Nutrición 1999-2006 y el precio de los alimentos a partir de la base trimestral de datos de precios de los alimentos. Aplicamos una regresión de los valores de glucemia con respecto al precio de los alimentos en el trimestre anterior, con control de la región del mercado y otras covariables. Examinamos asimismo si la asociación entre el precio de los alimentos y la glucemia variaba entre distintos grupos de ingresos. RESULTADOS: Tanto el precio de las frutas y verduras como el precio de los productos lácteos magros se asocian a la glucemia en las personas con diabetes de tipo 2. En concreto, un precio mayor de las frutas y verduras y de los productos lácteos se asocia a valores más altos de glu-cohemoglobina y de glucemia en ayunas tres meses después. La asociación entre el precio de los alimentos y la glucemia es mayor en las personas de ingresos bajos que en las de ingresos elevados, en la dirección esperada. CONCLUSIONES: Un precio mayor de los alimentos saludables se asocia a cifras más elevadas de glucemia en las personas con diabetes de tipo 2. Esta asociación fue especialmente pronunciada en las personas con diabetes de tipo 2 con ingresos bajos.

  13. Infección por Polyoma virus en un paciente con transplante renal: A propósito de un caso

    Directory of Open Access Journals (Sweden)

    Reyner Loza Munarriz

    2008-07-01

    Full Text Available La nefropatia por polyoma virus (NVBK esta siendo reconocida como causa importante de falla del injerto, usualmente confundido con rechazo agudo. A la fecha, no se han reportado casos en el Perú. Se presenta el caso de un niño trasplantado, 17 años de edad, quien presentó elevación persistente de la creatinina sérica en el primer mes postrasplante. Fue tratado como rechazo agudo. Al no presentar mejoría se realizó biopsia renal que mostró marcado daño del epitelio tubular con necrosis parcial y desprendimiento hacia la luz tubular y la presencia de células atípicas con escaso citoplasma e inclusiones intranucleares basofilicas, hallazgos compatibles con infección por virus BK. No se evidenció signos de rechazo. Además, en el papanicolao de orina, se encontró Decoy cells. La carga viral de virus BK fue elevada en sangre y en orina. Se suspendió el tratamiento con mofetil micofenolato, se redujo la dosis de tacrolimus y se inició el tratamiento con leflunomide 40 mg/dia. Seis meses después la función renal del paciente se estabilizó, pero sin mejoría completa quedando con una depuración de creatinina de 30 ml/min/1,73 m² SC. (Rev Med Hered 2008;19:123-127

  14. Conversando con...Momoyo Kaijima

    OpenAIRE

    Gómez Alonso, Carlos; Álvarez Isidro, Eva; Torres Barchino, Ana

    2017-01-01

    [ES] Momoyo Kaijima es profesora en la Facultad de Arte y Diseño de la Universidad de Tsukuba en la Prefectura de Ibaraki y profesora visitante en la ETH de Zürich, en Royal Academy of Fine Arts, en Rice School of Architecture y en Harvard GSD. A lo largo de los años, Atelier Bow Wow ha colaborado con Krešimir Rogina, arquitecto de Zagreb y socio de la firma internacional Penezic&Rogina, en la realización del Grožnjan International Summer School of Architecture, siendo Rogina el nexo indispen...

  15. Estudio electrofisiológico de la funcion neuromuscular en una poblacion intoxicada con plomo

    Directory of Open Access Journals (Sweden)

    O. M. Genovese

    1988-03-01

    Full Text Available Un grupo de 12 pacientes con cifras elevadas de plombemia y disminuidas de ALA D fueron estudiados electrofisiológicamente. Se efectuaron las siguientes determinaciones: amplitud de potencial evocado muscular; latencia distal motora; velocidad de conducción motora en los nervios mediano, ciático poplíteo externo y radial; velocidad de conducción sensitiva y amplitud del potencial evocado sensitivo de nervio en el mediano; exploración electromiográfica convencional; estudio de la transmisión neuromuscular, mediante estimulación repetitiva del nervio ciático popliteo externo a diversas frecuencias, con registro de la amplitud del potencial en músculo tibial anterior. Los datos obtenidos fueron comparados con controles. Se buscaron correlaciones entre las determinaciones bioquímicas y los diferentes hallazgos electrofisiológicos que a su vez se correlacionaron entre si y también con el tiempo de evolución de la intoxicación. Los hallazgos pusieron de manifiesto la presencia de neuropatía axonal junto a moderada desmielinización. La exploración de la transmisión neuromuscular mostró ocasional decremento o incremento de la amplitud del potencial muscular evocado, hechos que suponen cierta participación de la unión neuromuscular. Se halló correlación solamente entre disminución de ALA D y la disminución de las amplitudes del potencial sensitivo del mediano y el evocado muscular del mismo nervio y con la disminución de la VCM del nervio radial. Una débil relación se observó entre el tiempo de exposición y la disminución de la VCS del nervio mediano y VCM del radial. Las determinaciones bioquímicas y el tiempo de exposición transcurridos no probaron ser parámetros sensibles para determinar el grado de afectación del sistema nervioso periférico. El conjunto do hallazgos sugiere que el desarrollo de la neuropatía puede estar ligado a factores individuales de predisposición al daño nervioso.

  16. Prevalence of IgE antibodies to an extract from rubber tree (Hevea brasiliensis latex and recombinant pollen allergens (Phl P 1, Phl P 2, Phl P 5, Bet v 1 and Bet v 2 in the sera of Italian atopic patients

    Directory of Open Access Journals (Sweden)

    Renato E. Rossi

    2001-01-01

    Conclusions: The findings of the present study may support the concept that a high proportion of sera containing IgE to rBet v 2, rPhl p 1 and rPhl p 5 simultaneously contain antilatex IgE. Therefore, patients with specific IgE to these recombinant allergens with no history of current latex exposure may need additional evaluation.

  17. Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite

    Directory of Open Access Journals (Sweden)

    Zhao Y

    2017-07-01

    Full Text Available Yan Zhao,1,* Qiang Zhang,2,* Qingfeng Meng,3 Fenglian Wu,4 Lihua Zhang,1 Yao Tang,1 Yuanyuan Guan,1 Lixin An1 1Department of Allergy, The First Affiliated Hospital, Harbin Medical University, Harbin, 2Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Yantai Yuhuangding Hospital, Qingdao University, Yantai, 3Department of Ophthalmology, The First Affiliated Hospital, Harbin Medical University, Harbin, 4Department of Burn and Plastic Surgery, The First Affiliated Hospital of Qinhuangdao, Qinhuangdao, People’s Republic of China *These authors contributed equally to this work Abstract: Semiquantitative and rapid detection of specific IgE (sIgE with well clinical relevance to house dust mite (HDM are promising for prevalence rhinitis and asthma patients due to the increasing air pollution. However, the conventional IgE measurement systems are time-consuming, complicated and require special instruments. Herein, we overcome the above limitations of sIgE to HDM detection system by developing a quantum dot nanobeads-based lateral flow immunoassay and an image analysis procedure. The proposed detection system could semiquantitatively measure the IgE in a linear range of 0.2–10 U/mL. Moreover, there is a well correlation between the developed detection system and the clinical symptoms by a comparison study using 56 positive patients’ sera and 40 healthy control sera. The proposed detection system is simple, robust and easy-to-use and promising for in home test. Keywords: lateral flow immunoassay, quantum dots, house dust mite, IgE 

  18. Component-resolved microarray analysis of IgE sensitization profiles to Felis catus major allergen molecules in Russian cat-allergic patients.

    Science.gov (United States)

    Dolgova, Anna Sergeevna; Sudina, Anna Evgenevna; Cherkashina, Anna Sergeevna; Stukolova, Olga Alekseevna

    We aimed to determine the profile of IgE reactivity to three major cat allergens, Fel d 1, Fel d 2 and Fel d 4, in cat-allergic patients in the Moscow region in Russia. sIgE levels to recombinant proteins expressed in Escherichia coli (Fel d 1 and Fel d 4) and to Fel d 2 protein purified from cat serum were measured using a microarray method developed in our laboratory. Sera from 174 anonymous subjects with a positive reaction (≥0.35 IU/mL) to cat dander extract (e1, ImmunoCAP) and 56 negative controls were used for IgE testing. Fel d 1 was recognized by 92.5%, Fel d 2 by 29.9% and Fel d 4 by 39.1% of the tested patient sera. The sensitivity to these three proteins was approximately 98% compared to cat dander extract (correlation coefficient to ImmunoCAP is 0.94 with PPV = 0.99 and NPV = 0.95). These predictive values appeared to be even more statistically significant than the divergence between the ISAC IgE test and the extract-based singleplex ImmunoCAP. The combination of the three investigated proteins (Fel d 1, Fel d 2 and Fel d 4) is suitable for in vitro molecular (serological) diagnosis of cat allergy in this region as a complement to cat dander extract. Moreover, with this method, we found distinction between Fel d 2 and other Feline sIgEs formation.

  19. Relaciones interpersonales en el nuevo modelo de atención sanitarista al niño con dolor

    Directory of Open Access Journals (Sweden)

    Ana María Collado Madurga

    Full Text Available Introducción: el alivio del dolor debe considerase un derecho fundamental de la persona, porque el respeto a la dignidad humana exige que nadie sufra dolor innecesario. Objetivo: valorar cómo se establecía la relación de los profesionales con el paciente y representantes válidos, en la atención a niños con dolor, y si se ajustaban a un modelo de afrontamiento ético de las relaciones interpersonales y las valoraciones sobre virtudes y cualidades presentes en los profesionales que atienden niños con dolor. Métodos: se realizó una investigación descriptiva-transversal utilizando métodos cualitativos. Se realizaron encuestas a tutores válidos de niños que padecían dolor ingresados en el Hospital Pediátrico "Juan Manuel Márquez", y a profesionales de ese hospital de diferentes servicios que atienden a niños con dolor. Las valoraciones de los profesionales sobre estas relaciones se contrastaron con las que hicieron los representantes legales de los niños con dolor atendidos en el hospital. Resultados: el personal de salud considera generalmente (casi siempre correctas las valoraciones bioéticas en la relación médica con los enfermos y familiares. Los tutores válidos de los niños ingresados escogieron la alternativa siempre correcta en los procederes orientados desde la Bioética por parte del personal de salud. Las cualidades que caracterizan al personal médico y de enfermería son apreciadas en el rango de suficiente, y estas, con relación a cómo deberían estar presentes, las frecuencias no fueron lo suficientemente elevadas para esta profesión. Conclusiones: se constató que la ponderación de valores deseados para la profesión no alcanza las expectativas necesarias para considerar que se estén formando profesionales virtuosos. Los profesionales valoraron que no siempre se cumplen los procederes orientados desde la bioética, sin embargo los padres consideran que estos son cumplidos siempre.

  20. Perfil sintomático de los pacientes con Enfermedad Renal Crónica Estadio 4 y 5

    Directory of Open Access Journals (Sweden)

    Daniel Gutiérrez Sánchez

    Full Text Available Resumen Introducción: Los pacientes con Enfermedad Renal Crónica Avanzada (ERCA experimentan una gran variedad de síntomas que afectan negativamente la calidad de vida del paciente. Objetivos: Determinar la prevalencia e intensidad de síntomas y analizar su asociación con el filtrado glomerular (FG y la comorbilidad. Material y Método: Estudio transversal en el que se describe la sintomatología del paciente con ERCA en diálisis y prediálisis y se realiza un análisis de la asociación de los síntomas con el FG y la comorbilidad. Para la evaluación de síntomas se utilizó la versión española modificada de la Palliative care Outcome Scale-Symptoms Renal (POS-S Renal, un cuestionario validado para evaluar la sintomatología en esta población. La comorbilidad fue evaluada con el índice de comorbilidad de Charlson modificado (ICCm. Resultados: 180 pacientes con ERCA fueron incluidos en este estudio. Más del 44% de pacientes describieron debilidad, dolor, depresión y dificultad para dormir. Se encontraron diferencias significativas en ambos grupos para síntomas como la debilidad (p=0.027, poca movilidad (p=0.018, somnolencia (p=0.03, estreñimiento (p=0.015 y piernas inquietas (p<0.01. El declive de la función renal se asoció con la sintomatología (p=0,04. No encontramos asociación entre la comorbilidad y la sintomatología (p=0,15. Conclusiones: Los pacientes con ERCA sufren una elevada carga de síntomas. La evaluación y monitorización sistemática de síntomas mediante herramientas como el POS-S Renal puede ser útil en el ámbito clínico y en investigación. La integración de los Cuidados Paliativos en los Servicios de Nefrología puede contribuir a la optimización del manejo sintomático en esta población.

  1. Gammagrafía renal en niños con primera infección febril del tracto urinario

    Directory of Open Access Journals (Sweden)

    María Caridad Duarte Pérez

    2012-12-01

    Full Text Available Introducción: la infección del tracto urinario es una de las infecciones bacterianas más frecuentes en la infancia. Objetivos: conocer las alteraciones renales en la fase aguda de la primera infección febril del tracto urinario, y relacionarlas con las variables estudiadas. Métodos: se estudiaron 211 niños con diagnóstico microbiológico de primera infección febril del tracto urinario, a los cuales se les realizó gammagrafía renal con Tc-DMSA en la fase aguda de la infección. Los resultados de la gammagrafía se correlacionaron con la duración e intensidad de la fiebre antes del diagnóstico, los reactantes de la fase aguda (hemograma, eritrosedimentación y proteína C reactiva y los resultados de los estudios imaginológicos (ultrasonido renal y uretrocistografía miccional. Resultados: predominó el sexo femenino y el 82,4 % de los pacientes fueron menores de un año. El 54 % de los pacientes presentaron alteraciones gammagráficas, y el patrón de hipocaptación del radiofármaco fue el hallazgo que predominó en el 70 % de ellos. Se encontró asociación significativa al relacionar la duración e intensidad de la fiebre con las alteraciones renales. De los parámetros de laboratorio, la leucocitosis superior a 15 000, la eritrosedimentación acelerada y la proteína C reactiva elevada, tuvieron significación estadística con las alteraciones renales, y los hallazgos radiológicos detectados por el ultrasonido renal y uretrocistografía miccional se correlacionaron estadísticamente con la afectación renal detectada con la gammagrafía. Conclusiones: un porcentaje elevado de los pacientes presentó extensión de la infección al parénquima renal, y las variables clínicas, de laboratorio e imaginológicas estudiadas fueron parámetros orientadores de daño renal.

  2. Niveles de inmunoglobulinas IgA e IgM en recién nacidos y correlación con infección congénita

    Directory of Open Access Journals (Sweden)

    Rafael Ferrer Montoya

    1998-03-01

    Full Text Available Se realizó un estudio analítico y prospectivo en 600 recién nacidos, de los niveles sanguíneos de IgM e IgA y su relación con el riesgo de infección congénita. Se encontró elevada la IgM en 10 neonatos (1,7 % y la IgA en 18 (3 %. Se halló una buena correlación (OR y significancia estadística de IgM e IgA elevadas en los neonatos cuyas madres tuvieron líquido amniótico caliente y/o fétido, ruptura de las membranas de más de 24 horas y fiebre intraparto. También hubo una buena correlación (OR y significancia estadística de las Igs elevadas en neonatos con bronconeumonía y sepsis generalizada congénita. De los cultivos bacteriológicos existió una buena correlación (OR y significancia estadística en el hemocultivo y exudado faríngeo y las Igs elevadas y el residuo gástrico con la IgM elevada. Las bacterias grampositivas y gramnegativas aisladas en los neonatos tuvieron buena correlación (OR y significancia estadística, excepto para la IgA en las bacterias gramnegativas. Los 4 neonatos fallecidos por infección congénita tuvieron IgM elevadas.A prospective analytical study of IgM and IgA inmunoglobulin levels of 600 neonates and their correlation with congenital infection risks was made. 10 neonates (1.7 % presented a high IgM level whereas 18 (3 % had a raised IgA level. A good correlation (OR and statistical significance of raised IgA and IgM levels were found in newborns of mothers who had had hot and/or fetid amniotic fluid; ruptured membranes for over 24 hours and intralabor fever. Good correlation and statistical significance of high Igs were also present in neonates affected by bronchopneumonia and general congenital sepsis. As to the bacteriological cultures, good correlation and statistical significance was found in hemoculture and pharyngeal exudate, as well as raised Igs levels, and gastric residues with high IgM level. Gram-positive and gram-negative bacteria isolated from newborns presented good correlation

  3. Food irradiation - pros and cons

    International Nuclear Information System (INIS)

    1983-01-01

    The use of ionising radiation for food preservation is a much-disputed topic, both among experts and among consumers. Pros and cons of this issue were discussed in detail at the consumers' forum. Professor Dr. Johannes Friedrich Diehl, Director of the Institute for Biochemistry of the Food Research Centre, Karlsruhe, is a well-known supporter of the new method of food preservation; he sees advantages in the radiopreservation of food because, for example, losses due to inedibility are reduced, the danger of salmonellosis is decreased, just as the use of chemicals. He thinks this method to be without danger to health, shown by many years of experience. Opponents to food irradiation like Prof. Dr. Konrad Pfeilsticker, Professor for food science and food chemistry at the Bonn University deem the method to be unnecessary and raise the problem of qualitative changes caused in the food. In the course of the discussions, the pros and cons seemed to balance each other out. (orig./AJ) [de

  4. De paseo con los Bourbaki

    Directory of Open Access Journals (Sweden)

    Miquel Escudero

    2012-04-01

    Full Text Available Setenta y siete años después de la fundación del grupo Bourbaki, procedemos a una reflexión que puede ser útil para los estudiantes que acaso no sepan de su existencia, ni tan siquiera los de matemáticas. Sería interesante conocer que a este grupo se le debe el signo del vacío como conjunto. Con todo lo discutible que sea el método Bourbaki en su reinterpretación de la matemática, no cabe duda de su importante repercusión hasta el punto de que ha marcado una época. Hay un antes y un después tras su irrupción, ningún matemático de primera fila de la segunda mitad del siglo XX fue ajeno a su influjo, para encabezarlo o para reprobarlo. Comenzaron como una juvenil extravagancia, pero repleta de conocimientos y con decidida voluntad de aprehender el rigor de forma exhaustiva.

  5. Tratamiento del paciente con artrosis

    Directory of Open Access Journals (Sweden)

    Francisco Vargas Negrín

    2014-01-01

    Full Text Available El manejo terapéutico del paciente con artrosis tiene como objetivo disminuir la sintomatología dolorosa e inflamatoria, mejorar la capacidad funcional del paciente y la aplicación de intervenciones terapéuticas eficaces y lo más seguras posibles. Un enfoque centrado en el paciente implica su participación activa en el diseño del plan terapéutico y en la toma de decisiones informadas oportunas en todas las etapas de la enfermedad. La educación terapéutica, la actividad física y el ejercicio terapéutico junto con el control de peso, en caso de sobrepeso u obesidad, constituyen el núcleo central del tratamiento. Los autocuidados individuales y por los familiares son fundamentales en el control del día a día del paciente. El uso de terapias físicas, ayudas técnicas (bastón, etc. y de fármacos tipo analgésicos simples, opioides y antiinflamatorios tiene evidencias demostradas en el control del dolor, mejora la funcionalidad y la calidad de vida del paciente y una clara recomendación de uso en el tratamiento de la artrosis. La cirugía conservadora y la de reemplazo articular se indican en los casos en los que no se logran los objetivos terapéuticos en casos concretos.

  6. IgE profiles of Bermuda grass pollen sensitised patients evaluated by Phleum pratense allergens Phl P 1, 2, 4, 5, 6 , 7, 11, 12.

    Science.gov (United States)

    Rossi, Renato E; Monasterolo, Giorgio; Prina, Paolo; Coco, Giuseppe; Operti, Daniela; Rossi, Lucilla

    2008-06-01

    Despite the difference in geographical dominance of certain grasses, a high degree of allergenic similarity or cross-reactivity between Bermuda grass pollen (BGP) and timothy grass pollen (TGP) has been previously demonstrated. The aim of the present study was to ascertain the sensitisation to TGP in 411 patients known for their reactivity to BGP extracts by analysing their reactivity to crude timothy pollen extract and timothy pollen purified allergens, establishing their specific IgE-profiles. Using the immunoenzymatic CAP method we evaluated IgE-specific antibodies for BGP- and TGP- extracts and the timothy recombinant (r) and natural (n) allergens rPhl p 1, rPhl p 2, nPhl p 4, rPhl p 5, rPhl p 6, rPhl p 7, rPhl p 11, and rPhl p 12. BGP-IgE positive patients (median = 8.0 kUA/l, 2.8-22.2 kUA/l 25th-75th percentile) simultaneously had IgE positive results for TGP (100% of subjects)(median = 48.9 kUA/l, 19.8- > 100 kUA/l 25th-75th percentile) and high prevalence of sensitization to 6/8 Phleum pratense allergens (Phl p 1, 2, 4, 5, 6, 11, markers of genuine sensitisation to TGP) other than profilin and calcium binding protein. More than 72% of BGP allergic patients were co-sensitised to rPhl p 1, rPhl p 2, nPhl p 4, rPhl p 5, rPhl p 6. A decrease of total and specific IgE with patients' age was observed. Our data show that all BGP-allergic patients simultaneously exhibit higher IgE antibody levels to recombinant and natural P. pratense allergens as well as to crude TGP extract. This suggests that when choosing an immunotherapeutic regimen for BGP-sensitised patients (after establishing their IgE profile via purified TGP-allergens), subcutaneous or sublingual TGP-extract vaccines in appropriate doses, in order to influence T epitope specificity, might be beneficial. Though extremely uncommon, in cases where a patient is exclusively BGP allergen-sensitised, BGP-extract therapy is the appropriate therapeutic response.

  7. Determination of IgE antibodies to the benzylpenicilloyl determinant: a comparison of the sensitivity and specificity of three radio allergo sorbent test methods.

    Science.gov (United States)

    Garcia, J J; Blanca, M; Moreno, F; Vega, J M; Mayorga, C; Fernandez, J; Juarez, C; Romano, A; de Ramon, E

    1997-01-01

    The quantitation of in vitro IgE antibodies to the benzylpenicilloyl determinant (BPO) is a useful tool for evaluating suspected penicillin allergic subjects. Although many different methods have been employed, few studies have compared their diagnostic specificity and sensitivity. In this study, the sensitivity and specificity of three different radio allergo sorbent test (RAST) methods for quantitating specific IgE antibodies to the BPO determinant were compared. Thirty positive control sera (serum samples from penicillin allergic subjects with a positive clinical history and a positive penicillin skin test) and 30 negative control sera (sera from subjects with no history of penicillin allergy and negative skin tests) were tested for BPO-specific IgE antibodies by RAST using three different conjugates coupled to the solid phase: benzylpenicillin conjugated to polylysine (BPO-PLL), benzylpenicillin conjugated to human serum albumin (BPO-HSA), and benzylpenicillin conjugated to an aminospacer (BPO-SP). Receiver operator control curves (ROC analysis) were carried out by determining different cut-off points between positive and negative values. Contingence tables were constructed and sensitivity, specificity, negative predictive values (PV-), and positive predictive values (PV+) were calculated. Pearson correlation coefficients (r) and intraclass correlation coefficients (ICC) were determined and the differences between methods were compared by chi 2 analysis. Analysis of the areas defined by the ROC curves showed statistical differences among the three methods. When cut-off points for optimal sensitivity and specificity were chosen, the BPO-HSA assay was less sensitive and less specific and had a lower PV- and PV+ than the BPO-PLL and BPO-SP assays. Assessment of r and ICC indicated that the correlation was very high, but the concordance between the PLL and SP methods was higher than between the PLL and HSA or SP and HSA methods. We conclude that for quantitating IgE

  8. Stigning i praevalensen af specifikt IgE imod luftvejsallergener i en dansk voksenpopulation--to tvaersnitsstudier i 1990 og 1998. Allergiundersøgelsen i Glostrup

    DEFF Research Database (Denmark)

    Linneberg, Allan R; Nielsen, Niels Henrik; Madsen, Flemming F

    2002-01-01

    E to aeroallergens had increased in an adult general population over an 8-year period. MATERIAL AND METHODS: Two cross-sectional surveys were carried out in 1990 and 1998. A screening questionnaire on respiratory symptoms sent to random samples of 15-41-year-old subjects living in Copenhagen (Denmark) preceded both...... adjustment for changes in questionnaire variables on life-style and home environment. The clinical significance of this increase was underlined by a corresponding increase in the prevalence of allergic rhinitis symptoms associated with specific IgE positivity. DISCUSSION: We found that the prevalence...

  9. Creencias y conductas de corrección en pacientes con hipertensión arterial

    Directory of Open Access Journals (Sweden)

    Genoveva Granados

    2008-01-01

    Full Text Available El presente estudio analítico-transversal tiene como principal objetivo analizar en pacientes hipertensos la información que habían recibido sobre las causas que provocan la hipertensión, las creencias generadas al respecto sobre su propia enfermedad, y las pautas de actuación en caso de tensión elevada. Igualmente, el estudio analiza la relación entre creencias y la práctica de pautas de autocuidado. Participaron en el estudio 171 pacientes hipertensos con tratamiento farmacológico, rango de edad entre 20 y 65 años. Los resultados indican una importante ausencia de relación entre los informes de creencias y los informes de acciones de autocuidado que los pacientes dicen haber llevado a cabo, y ponen de manifiesto la presencia de ciertas conductas de riesgo en el repertorio de la mayoría de los pacientes que pueden incidir en los resultados del tratamiento de la hipertensión. Se discute la importancia de estos novedosos resultados y la repercusión sobre las pautas de atención en atención primaria a este tipo de pacientes.

  10. Control repetitivo digital de sistemas con frecuencia incierta o variante en el tiempo

    Directory of Open Access Journals (Sweden)

    Ramon Costa-Castelló

    2012-07-01

    Full Text Available Resumen: El control repetitivo digital es una técnica basada en el principio del modelo interno que permite seguir y/o rechazar señales periódicas. Una hipótesis clave en los diseños tradicionales de control repetitivo es que la frecuencia de tales señales es constante y conocida, siendo su principal desventaja la elevada degradación de prestaciones que aparece cuando dicha frecuencia es incierta o varía con el tiempo. En este trabajo se presenta una revisión de las principales estrategias introducidas hasta la fecha para resolver este problema. Abstract: Digital repetitive control is an internal model principle-based control technique that aims at tracking/rejecting periodic signals. A key assumption in traditional repetitive control designs is that the frequency of such signals is constant and known, its main drawback being the dramatic loss of performance that occurs when this frequency is uncertain or time-varying. This article reviews the most relevant proposals introduced so far to overcome this problem. Palabras clave: Control repetitivo, Sistemas de control lineal, Sistemas de tiempo discreto, Regulación de salida, Keywords: Learning control, Linear control systems, Discretetime systems, Output regulation

  11. Effective anthelmintic therapy of residents living in endemic area of high prevalence for Hookworm and Schistosoma mansoni infections enhances the levels of allergy risk factor anti-Der p1 IgE

    Directory of Open Access Journals (Sweden)

    Sabrina S. Campolina

    2015-01-01

    Full Text Available In this work were investigated the relationship between Hookworm/Schistosoma mansoni infections and allergy related risk factors in two endemic areas with distinct prevalence of infections and co-infection. The intensity of infections, eosinophilia, allergy risk factors, infections status and anti-Der p1 IgE levels before and 2 years (population 1 and 3 years (population 2 after anthelmintic treatment, were evaluated. It was observed that the population with lower prevalence and intensity of infection (population 2 had lower eosinophils counts (>600/mm3 and higher animal contact than the population with higher parasites intensity (population 1. After anthelmintic treatment the intensity of S. mansoni single infection decreased, but no changes were observed in Hookworm and co-infected individuals. The anthelmintic treatment also enhanced anti-Der p1 IgE optical density in ELISA on the subgroups that became negative for helminth infection regardless of their previous infection condition in population 1. Facing that, we evaluated the anti-Der p1 IgE reactivity index, and the ratio (after/before treatment was significantly higher in patients co-infected before treatment. On the other hand, no association between anti-Der p1 IgE reactivity index and the intensity of infections were observed. In conclusion, effective anthelmintic therapy of subjects from endemic areas with high prevalence of Hookworm and S. mansoni infections enhances anti-Der p1 IgE levels.

  12. Fabricación de piezas de fundición con grafito esferoidal en molde metálico

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    Urrestarazu, A.

    2013-10-01

    Full Text Available The features and suitability of high requirements ductile iron castings production using metallic moulds have been studied in the present work. The structural and mechanical properties of the produced castings have been analysed and compared to the corresponding ones but fabricated using green sand moulds according to a conventional production process. The higher cooling rate in the metallic moulds is the main cause for the appearance of the detected structural changes in castings. The mechanical and microstructural properties obtained directly on castings are remarkable due to the higher nodule count among other factors. Finally, the benefits and inconveniences found in this kind of production methodology using metallic moulds are also discussed.En este trabajo se estudia el empleo de moldes metálicos o permanentes para la fabricación de piezas de fundición esferoidal con elevados requerimientos funcionales y se analizan sus propiedades, comparándolas con piezas obtenidas utilizando moldes de arena de sílice, de acuerdo con las metodologías más habituales para este tipo de procesos. La elevada velocidad de solidificación y el posterior enfriamiento rápido de la austenita formada en estado sólido se erigen como los principales factores diferenciadores que originan las modificaciones estructurales detectadas en las piezas. Las propiedades físicas, mecánicas y microestructurales obtenidas directamente sobre pieza son destacables debido, entre otros aspectos, al gran número de esferoides grafíticos obtenidos en las piezas. Se discuten también las ventajas e inconvenientes encontrados en esta metodología de producción que emplea moldes fabricados con una aleación metálica específica.

  13. Producción y valor nutricional del cultivo de morera (Morus alba L. con intercalamiento de leguminosas

    Directory of Open Access Journals (Sweden)

    María Judith Delgado

    2012-07-01

    Full Text Available Una de las principales preocupaciones en la alimentación animal es la búsqueda de alternativas rentables para el productor y eficientes desde el punto de vista nutricional para los animales. Para que el volumen de la producción de estos alimentos sea el adecuado, se han utilizado prácticas como las planteadas en la revolución verde, que incluyen el uso de fertilizantes nitrogenados químicos, lo que ha traído secuelas negativas sobre el medioambiente. En la búsqueda de nuevas alternativas nutricionales se encontraron algunos forrajes, como la morera (Morus alba L., que tiene un alto nivel de proteína, entre otras propiedades nutricionales; pero, alternativas nutricionales como ésta requieren fertilización nitrogenada en elevadas cantidades.Con el interés de generar alternativas amigables con el medioambiente y que además logren mantener o incrementar el volumen de producción y el valor nutricional de la morera, surgió la idea de estudiar la intersiembra de este arbusto con leguminosas, como arveja (Pisum sativum L., alfalfa (Medicago sativa L y fríjol (Phaseolus vulgaris L., analizando variables nutricionales y parámetros de crecimiento del cultivo de morera. Además, se trabajó con un grupo de fertilización nitrogenada y un grupo de control (sin modificación alguna del cultivo. Al finalizar el estudio se encontraron diferencias estadísticamente significativas (p<0.05 para la mayoría de parámetros, exceptuando la variable altura, que no presentó diferencias importantes (p>0.05.

  14. Cons ICARUS, TIGER and Fascism

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    Janez Vrečko

    2010-12-01

    Full Text Available Like the scientists of their time, Russian artists in the 1920s considered gravity the central problem – a view which points to the close harmony between modern physics and the avant-garde. It was only with the constructivist movement that the Icarus revolution grasped the principles of the “mobile philosophy” (3.651 which was almost at the same time recognised by modern physics as well. The static view of the world became obsolete, space and time were no longer absolute values. It was necessary to transcend Euclidean geometry, shake off the political ʻshackles on one’s hands’ and surrender to Lisicki’s imaginary space, where “At 2000 metres in the air / there is no more perspective” (Integrals 276.  Kosovel’s Icarus project accorded with Tatlin’s, and both of them accorded with the quintessential aims of the constructivist movement. It is no accident that Kosovel wished to name one of his poetry collections The Dream of Icarus. Poems on the Icarus theme, such as “Cons Icarus”, “Evacuation of the Spirit”, “Eh, Hey”, “A Heart in Alcohol” etc. belong to the group of Kosovel’s conses which follow his “mobile philosophy” (3.650 and “letters growing into space” (Int. 282.  The question “Man, do you want up in the air?” (Int. 128 will remain a question until the moment when man is finally ready to transcend the existing boundary and dive “beyond”. Hence Kosovel’s clear-cut contrast between the “green windows of an illuminated / express on a viaduct”, which moves horizontally and is, like a water current, subject to the earth’s gravity, and “the spirit in space”, whose direction of motion is “the perpendicular of the spirit”, atectonicity. “The spirit burns in space”: fire is an element that knows vertical movement alone, the only one of the elements to outgrow and transcend the earth’s gravity, therefore it is associated with another mythological figure important for

  15. Fare astronomia con piccoli telescopi

    CERN Document Server

    Gainer, Michael K

    2007-01-01

    Non sono necessariamente richiesti strumenti mastodontici per produrre risultati scientificamente validi nel campo dell’astronomia. Anche l’astrofilo dotato di un piccolo telescopio, con un diametro di soli 8-9 cm, può contribuire alla scienza del cielo realizzando utili osservazioni del Sole, della Luna, dei pianeti, delle comete, degli asteroidi, delle stelle doppie o variabili, delle nebulose e degli ammassi stellari. Il manuale di M.K. Gainer spiega quale sia la dotazione minima (un piccolo telescopio, un computer, una semplice fotocamera digitale), come utilizzarla, e quali siano le tecniche appropriate da adottare nelle osservazioni. Offre inoltre schemi per interpretare e ridurre i dati raccolti, nonché schede da compilare e da spedire ai centri di raccolta internazionali. Questo libro è il passaporto grazie al quale l’astrofilo può entrare a pieno titolo nel mondo affascinante della scienza astronomica.

  16. Factores de riesgo inmunoepidemiológicos en niños con infecciones respiratorias recurrentes

    Directory of Open Access Journals (Sweden)

    Mayra Pérez Sánchez

    2011-09-01

    Full Text Available Introducción: las infecciones respiratorias recurrentes que se presentan en edades tempranas tienen una elevada morbilidad y existen numerosos factores que contribuyen a su desarrollo. Objetivo: identificar los factores de riesgo inmunoepidemiológicos que contribuyeron a la aparición de las infecciones respiratorias recurrentes. Métodos: estudio observacional descriptivo longitudinal retrospectivo en un grupo de niños procedentes de Ciudad de la Habana y La Habana que ingresaron por infecciones respiratorias recurrentes y que asistieron a la consulta de inmunología en el período comprendido de enero de 2005 a diciembre de 2007 en el Hospital Maternoinfantil "Ángel Arturo Aballí". Resultados: la edad preescolar, el sexo masculino, la lactancia materna no efectiva, la alergia, el bajo peso al nacer, el humo de tabaco en el ambiente, la asistencia a círculos infantiles y el hacinamiento fueron los factores de riesgo más frecuentes. Los trastornos de la respuesta inmune mayormente encontrados fueron el defecto inmune celular, el defecto inmune humoral y el trastorno fagocítico. Conclusiones: los factores de riesgo inmunoepidemiológicos encontrados en la muestra de estudio fueron: la edad comprendida entre los 1 a 5 años con predominio del sexo masculino, la lactancia materna no efectiva, la alergia, el bajo peso al nacer, el humo de tabaco en el ambiente, el hacinamiento y la asistencia a los círculos infantiles. Estos se acompañan de defectos de la respuesta inmune con predominio de la rama celular.

  17. Determination of IgE antibodies to Polistes dominulus, Vespula germanica and Vespa crabro in sera of patients allergic to vespids.

    Science.gov (United States)

    Blanca, M; Garcia, F; Miranda, A; Carmona, M J; Garcia, J; Fernandez, J; Terrados, S; Vega, J M; Juarez, C

    1991-02-01

    The study was undertaken to investigate the presence of IgE antibodies to Polistes dominulus (PD), Vespula germanica (VG) and Vespa crabro (VC) in a large group of sera belonging to patients sensitized to Vespids in Spain. RAST values showed that although the majority of patients had IgE antibodies to PD, VG and VC, there was a marked predominance of PD. These results were related to the distribution of the insect in the areas where the sera were obtained. Due to geographical and insect distribution differences, the whole area was divided into three zones: Central, East and South. Comparison of the positive RAST values obtained indicated that, although the positivity to PD predominated over VG and this over VC, there were significant differences in percentage positivities to each vespid in the different regions studied. The results of the RAST absorption studies indicated that in most instances patients were originally sensitized to one vespid and were RAST positive to the other venoms due to cross-reactivity. Only in a minority of cases were coexisting antibodies to two insects present. These results show that PD and VG are the important vespids followed to a lesser extent by VC. This study provides relevant information concerning insect distribution sensitivity in a European country.

  18. Asp f6, an Aspergillus allergen specifically recognized by IgE from patients with allergic bronchopulmonary aspergillosis, is differentially expressed during germination.

    Science.gov (United States)

    Schwienbacher, M; Israel, L; Heesemann, J; Ebel, F

    2005-11-01

    Aspergillus fumigatus is a pathogenic mould causing allergic and invasive respiratory diseases. Allergic bronchopulmonary Aspergillosis (ABPA) is a severe pulmonary complication resulting from hypersensitivity to A. fumigatus proteins. Aspergillus allergen Asp f6 is recognized by IgE from ABPA patients, but not from sensitized individuals, a fact that can be used to differentiate between these two groups of allergic patients. Proteins from hyphae, resting and germinating conidia of A. fumigatus were compared by SDS-PAGE. Protein identification was performed using MALDI-TOF mass spectrometry. Recombinant A. fumigatus allergens were used to isolate specific monoclonal antibodies (mab) from a hybridoma bank generated against Aspergillus proteins. A hyphae-specific 23 kDa A. fumigatus protein was identified as the allergen Asp f6/manganese-dependent superoxide dismutase (MnSOD). Differential expression of MnSOD was confirmed by immunoblot using a specific mab. In contrast, Asp f8 another intracellular, but not ABPA-specific allergen, was detected in hyphae and conidia. Aspergillus fumigatus is able to colonize its environment by the formation of hyphae. Hyphae are found in the lung of ABPA patients, but not in patients suffering from atopic asthma. Our finding that Asp f6 is specifically expressed in hyphae might explain why an IgE response to Asp f6 is specific for ABPA patients.

  19. Deoxynivalenol (DON) is toxic to human colonic, lung and monocytic cell lines, but does not increase the IgE response in a mouse model for allergy

    International Nuclear Information System (INIS)

    Instanes, Christine; Hetland, Geir

    2004-01-01

    We examined whether the common crop mycotoxin deoxynivalenol (DON) from Fusarium species is toxic to human colonic (Caco-2), lung (A549) and monocytic (U937) cell lines. Moreover, since DON reportedly induces increased levels of Th2 cytokines and total IgE, and we have observed that mould extracts adjuvated allergy development in mice, possible adjuvant effect of DON on allergy was studied in a mouse model. For all the cells, exposure to DON for 24 h reduced cellular protein synthesis, proliferation and survival rate dose-dependently. In addition, production of IL-8 in the U937 cell line increased up to eight-fold at levels of DON just lower than the most toxic one, suggesting that IL-8 can be used as an additional index for cytotoxicity in mononuclear phagocytes. However, DON did not increase levels of allergen-specific IgE or IgG1 in the mouse model for allergy. These results suggest that DON, when inhaled or ingested, may have toxic effect on human alveolar macrophages and epithelial cells in lungs and colon, but does not increase the allergic response to allergens

  20. Establishment of a novel high-affinity IgE receptor-positive canine mast cell line with wild-type c-kit receptors

    International Nuclear Information System (INIS)

    Amagai, Yosuke; Tanaka, Akane; Ohmori, Keitaro; Matsuda, Hiroshi

    2008-01-01

    Much is known regarding participations of mast cells with innate and acquired immunity by secreting various cytokines and chemical mediators. However, details of mast cell biology still remain unclear. In this study, we successfully established a novel growth factor-independent mast cell line (MPT-1) derived from canine mast cell tumor. MPT-1 cells manifested factor-independent proliferation as floating cells containing a large amount of histamine, as well as chymase-like dog mast cell protease 3, in cytosolic granules. Particularly, MPT-1 cells expressed high-affinity IgE receptors (FcεRI) and wild-type c-kit receptors. Degranulation of MPT-1 cells was induced not only by stimulation with calcium ionophore but also by cross-linkage of the surface IgE. Given that MPT-1 is the first mast cell line with FcεRI which has no c-kit mutations, MPT-1 cells may provide great contribution for investigation of IgE-mediated activation mechanisms of mast cells, leading to development of effective treatment for allergic disorders

  1. Adaptación española del Inventario para la Depresión de Beck-II (BDI-II: 3. Propiedades psicométricas en pacientes con trastornos psicológicos

    Directory of Open Access Journals (Sweden)

    JESÚS SANZ

    2005-01-01

    Full Text Available Se presentan datos sobre la fiabilidad y validez de la adaptación española del Inventario para la Depresión de Beck-II (BDI-II; Beck, Steer y Brown, 1996, obtenidos con una muestra de 305 pacientes ambulatorios con diversos diagnósticos psicopatológicos según el DSM-IV. El coeficiente alfa de fiabilidad fue alto (alfa = 0,89. Las correlaciones con otras medidas autoaplicadas y heteroaplicadas de depresión fueron elevadas y significativamente mayores que la correlación con una medida de ansiedad, lo que avala la validez convergente y discriminante del BDI-II, respectivamente. Respecto a la validez de criterio, los resultados demostraron que los pacientes diagnosticados con un trastorno depresivo mayor tenían niveles de depresión más elevados, medidos con el BDI-II, que los pacientes de otros grupos diagnósticos, aunque no hubo diferencias con los pacientes con trastornos de personalidad. Finalmente, la validación factorial del BDI-II proporcionó una solución bifactorial (factor somático- motivacional y factor cognitivo que coincide con la hallada en estudios previos. Se concluye que el BDI es un instrumento válido de detección y cuantificación de síntomas depresivos en pacientes, si bien su utilidad como herramienta para el diagnóstico diferencial de la depresión es una cuestión pendiente de investigación.

  2. Desarrollo y optimización de recubrimientos de superaleaciones con adición de cargas cerámicas y elementos reactivos obtenidos por proyección térmica para aplicaciones de resistencia a oxidación a alta temperatura y al desgaste

    OpenAIRE

    Cervera González, Iván

    2011-01-01

    El objetivo principal del presente trabajo es la obtención de recubrimientos con una composición optimizada para aplicaciones de elevada resistencia a alta temperatura y al desgaste, proyectados térmicamente sobre acero al carbono AISI 1110, mediante la técnica de oxifuel, basados en el sistema NiCrAlY/Al2O3. Estos recubrimientos deben ser capaces de proteger adecuadamente a un material de escasa resistencia mecánica y térmica para el rango de temperaturas analizado, que se sitúa entre 850 – ...

  3. Asociación del anticuerpo anti-C1q con las manifestaciones clínicas, hematológicas e inmunológicas en el Lupus Eritematoso Sistémico : Estudio observacional retrospectivo de 135 casos.

    OpenAIRE

    Córica, María Emilia

    2012-01-01

    : Las concentraciones de anticuerpo anti-C1q están elevadas en los pacientes afectados de LES. Según algunos estudios la presencia de este anticuerpo está asociada a la nefropatía lúpica, sin embargo en otros estudios esta relación no se comprobó. En nuestro trabajo relacionamos la positividad del anticuerpo anti-C1q con las manifestaciones clínicas, hematológicas e inmunológicas de la enfermedad. A diferencia de estudios previos sólo pudimos establecer una relación estadísticamente significa...

  4. Interleukin-7 (IL-7) enhances class switching to IgE and IgG4 in the presence of T cells via IL-9 and sCD23.

    Science.gov (United States)

    Jeannin, P; Delneste, Y; Lecoanet-Henchoz, S; Gretener, D; Bonnefoy, J Y

    1998-02-15

    Interleukin-7 (IL-7) is a B-cell growth factor produced by both bone marrow stroma cells and follicular dendritic cells (FDCs) located in primary lymphoid follicles and germinal centers. In this study, we have evaluated the role of IL-7 on human Ig class switching. IL-7 was added to peripheral blood mononuclear cells (PBMCs) or tonsillar B cells in the absence or presence of IL-4 and/or anti-CD40 monoclonal antibody (MoAb). Alone, IL-7 did not affect Ig production by PBMCs or by anti-CD40 MoAb-stimulated B cells. Rather, IL-7 potentiated IL-4-induced IgE and IgG4 production by PBMCs. In parallel, IgG3 production was also enhanced but to a lesser extent, whereas the production of the other isotypes was unaltered. The activity of IL-2, IL-9, or IL-15, which share usage of the common gamma chain for signaling, was also assessed. IL-9, like IL-7, potentiated mainly IgE and IgG4 production by IL-4-stimulated PBMCs. IL-15, in contrast, was ineffective, whereas IL-2 enhanced the production of all isotypes. More precisely, IL-7 potentiation of IgE and IgG4 production required the presence of T cells and was accompanied by an increase of the expression of two soluble molecules favoring preferentially IgE and IgG4 synthesis: CD23 (sCD23) and IL-9. Moreover, neutralizing anti-CD23 and anti-IL-9 antibodies partly inhibited the increase of IgE synthesis induced by IL-7. Thus, IL-7 produced locally in the germinal centers by FDCs may interact with T cells and potentiate human IgE and IgG4 switching by favoring IL-9 and sCD23 production.

  5. Depresion en pacientes con alteraciones del tiroides

    OpenAIRE

    Radanovic-Grguric´, Ljiljana; Filakovic´, Pavo; Barkic´, Jelena; Mandic´, Nikola; Karner, Ivan; Smoje, Juraj

    2003-01-01

    Nuestro estudio fue realizado en un grupo de 53 mujeres con disfunción tiroidea y 28 mujeres con depresión mayor. Empleamos la Escala de la Depresión de Hamilton, la Escala de Autoevaluación de la Depresión de Zung y la Escala sobre la Impresión Clínica Global. Los resultados del estudio demuestran que la mayoría de los pacientes con disfunción tiroidea se mostraron clínicamente significativos en cuanto al trastorno depresivo. Los episodios depresivos son más frecuentes en pacientes con hipot...

  6. Aprende Ajedrez con Rey - Parte 2

    OpenAIRE

    ESTÉVEZ MONTERO, RAÚL; Lloret Mauri, Jaime

    2016-01-01

    Es una pieza audiovisual creada con el objeto de atraer la atención de los niños de muy corta edad con el ajedrez y familiarizarlos con todas sus piezas y movimientos. Es una animación dirigida a un público infantil presentada por dibujos animados en 2D, en la que se ha intentado respetar en todo momento el argot de la comunidad ajedredística. En este video se presenta la segunda parte. Estévez Montero, R.; Lloret Mauri, J. (2016). Aprende Ajedrez con Rey - Parte 2. http://hdl.handle.net/1...

  7. Aprende Ajedrez con Rey - Parte 1

    OpenAIRE

    ESTÉVEZ MONTERO, RAÚL; Lloret Mauri, Jaime

    2016-01-01

    Es una pieza audiovisual creada con el objeto de atraer la atención de los niños de muy corta edad con el ajedrez y familiarizarlos con todas sus piezas y movimientos. Es una animación dirigida a un público infantil presentada por dibujos animados en 2D, en la que se ha intentado respetar en todo momento el argot de la comunidad ajedredística. En este video se presenta la primera parte. Estévez Montero, R.; Lloret Mauri, J. (2016). Aprende Ajedrez con Rey - Parte 1. http://hdl.handle.net/1...

  8. Consideraciones sobre la evolución de la respuesta inmunitaria Th2 y sus posibles relaciones con parasitosis y alergia

    Directory of Open Access Journals (Sweden)

    Luis Caraballo

    2012-03-01

    El desarrollo de este equilibrio debió incluir adaptaciones de ambos organismos y la supervivencia del parásito podría ser el resultado de la adquisición de mecanismos supresores de la respuesta defensiva, la selección de los huéspedes con menor intensidad de la respuesta de tipo 2, o ambas. Esto, a su vez, sugiere que aunque las infecciones helmínticas hayan influido en la conformación de la inmunidad de tipo 2, no han sido una fuerza selectiva importante en el caso particular de la respuesta alérgica que, a su vez, está más ligada a una exagerada respuesta Th2/IgE.   DOI: http://dx.doi.org/10.7705/biomedica.v32i1.419

  9. The Biological Effect of Fast Neutrons and High-Energy Protons; Effets Biologiques des Neutrons Rapides et des Protons de Haute Energie; Biologicheskoe dejstvie bystrykh nejtronov i protonov vysokikh ehnergii; Efectos Biologicos de los Neutrones Rapidos y de los Protones de Elevada Energia

    Energy Technology Data Exchange (ETDEWEB)

    Moskalev, Ju. I.; Petrovich, I. K.; Strel' cova, V. N.

    1964-03-15

    peut subir des variations sensibles en fonction du temps pour un seul et meme indice. Longtemps apres une irradiation par des neutrons rapides ou par des protons, on constate chez le rat l'apparition de tumeurs benignes et malignes dans divers tissus, notamment dans le tissu hematopoie tique, les glandes mammaires, l'hypophyse, l'uterus, les ovaires, la prostate, les testicules, la thyroide, la glande cervicale, le pancreas, les glandes surrenales, le foie, les reins, les poumons, le tractus gastro-intestinal, le tissu cellulaire sous-cutane, les ganglions lymphatiques, la vessie, etc. La frequence globale des tumeurs et le nombre des apparitions simultanees de neoplasmes en plusieurs endroits ont ete deux a trois fois plus eleves chez les femelles que chez les males. La dose minimum de neutrons necessaire pour provoquer une tumeur des glandes mammaires semble legerement inferieure a 42,5rad. La frequence des tumeurs de l'hypophyse est la plus elevee lorsque la dose est de 42,5 rad. A cette meme dose,. on constate l'apparition de leucoses et de tumeurs de la thyroide. Lors d'une irradiation par des protons, la dose minimum necessaire pour provoquer une tumeur est de l'ordre de 250 rad pour le tissu hematopoietique et la thyroide, de 300 rad pour les testicules et de 100 rad pour la prostate et le tissu cellulaire sous-cutane. Lors d'une irradiation par les neutrons rapides, les tumeurs des testicules, du gros intestin, des reins et du foie sont plus frequentes pour 42,5 a 85 rad et les tumeurs de la peau et du tissu cellulaire sous-cutane pour 85 rad. Dans le cas d'une irradiation par neutrons rapides, les tumeurs se produisent a des doses inferieures (d'environ 3 a 5 fois) aux doses de protons. (author) [Spanish] Los autores presentan los resultados de experimentos efectuados con miras a comparar el efecto de los neutrones rapidos y el de los protones de elevada energia (500 MeV) sobre la duracion de la vida, las caracteristicas de la sangre periferica, asi como la

  10. Plantas cubanas con efecto antiinflamatorio

    Directory of Open Access Journals (Sweden)

    Ada Ivis Regalado Veloz

    Full Text Available La actividad antiinflamatoria suscita gran interés científico en el área farmacológica, debido a que muchas enfermedades en su evolución cursan por procesos inflamatorios (artritis reumatoide, ateroesclerosis, cáncer, diabetes, gota, asma, dermatitis, trastornos neurodegenerativos y diversas dolencias menores. Las enfermedades inflamatorias constituyen un problema de salud importante, debido a la falta de medicamentos eficaces y seguros para su uso por periodos prolongados. Hoy en día se trabaja en la búsqueda de alternativas de antiinflamatorios más seguros, en el que las plantas medicinales, una de las formas más antiguas de tratamiento, constituyen una elección a considerar. En este trabajo se realizó una revisión bibliográfica, sobre especies de plantas que crecen en Cuba que le reportan propiedades farmacológicas como antinflamatorios. En la revisión de la literatura se utilizó la base de datos Medline (vía PubMed, así como revistas nacionales desde el periodo de 2000 hasta el presente, con las palabras claves "inflamación" y "plantas cubanas antiinflamatorias" o "actividad antiinflamatoria" y "plantas medicinales".

  11. Hospitalidad, con y sin papeles

    Directory of Open Access Journals (Sweden)

    Ana Paula Penchaszadeh

    Full Text Available Resumen El objetivo de este artículo es vincular el trabajo sobre el archivo de Jacques Derrida con la experiencia de la hospitalidad. Se intentará mostrar que, por un lado, se trata siempre de los papeles, de la legitimidad que éstos otorgan o no tanto a nivel filosófico (deseo de poseer los papeles que autoricen tal o cual decisión interpretativa, como a nivel político ("tener papeles" como el principio básico de todo derecho a tener derechos, de todo derecho a la comunidad. Mas también, por otro lado, se intentará pensar aquello que arruina la idea misma de tener o no tener (papeles, la idea de propiedad, aquello que hace imposible fundar una decisión o identidad en última instancia y, por ende, una soberanía, una frontera. La hospitalidad, la llegada inminente del otro, representa un desafío político y ético para la filosofía: pues no se t