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Sample records for con cepas intermedias

  1. Primer aislamiento en Argentina de Staphylococcus aureus resistente a la meticilina adquirido en la comunidad con sensibilidad intermedia a la vancomicina y no sensibilidad a la daptomicina

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    Laura Errecalde

    Full Text Available Describimos el primer caso en Argentina de Staphylococcus aureus resistente a la meticilina adquirido en la comunidad con sensibilidad intermedia a la vancomicina y no sensibilidad a la daptomicina. Caso clínico: Paciente masculino, con antecedente de insuficiencia renal crónica en hemodiálisis y osteosíntesis de cadera debido a una fractura. Se internó por síndrome febril persistente luego del desplazamiento de la prótesis por un traumatismo. Se aisló de hemocultivos S. aureus resistente a la meticilina adquirido en la comunidad. Durante el tratamiento con vancomicina y daptomicina, se observó un aumento gradual de la CIM de vancomicina de 1 µg/ml (VSSA a 2 µg/ml (h-VISA y a 4 µg/ml (VISA, y la emergencia de no sensibilidad a daptomicina (CIM = 4 µg/ml. Al suspender la vancomicina y la daptomicina, la cepa revirtió al fenotipo de sensibilidad a ambas drogas. Es mandatorio evaluar por CIM la sensibilidad a vancomicina y a daptomicina intratratamiento cuando estas drogas se usan como terapia.

  2. Marcadores fenotípicos de atenuación en cepas de virus Junín recuperadas de individuos vacunados con la cepa Junín Candid#1

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    Graciela S. Gamboa

    2013-08-01

    Full Text Available La Fiebre Hemorrágica Argentina es una enfermedad producida por el virus Junín. Para la prevención de esta enfermedad se obtuvo una vacuna efectiva denominada Candid#1. Durante un ensayo clínico realizado en el INEVH, dos cepas de virus Junín fueron aisladas de sangre periférica de dos voluntarios mediante co-cultivo de células mononucleares. El objetivo de este trabajo fue comparar las características fenotípicas de atenuación de esas dos cepas recuperadas de humanos con las de la vacuna Candid#1 utilizando los indicadores de atenuación desarrollados por Contigiani y Sabattini en 1977. A tal fin se midieron los índices de letalidad, infección y protección en cobayos y ratones de diferentes edades. Las tres cepas investigadas resultaron letales para ratones recién nacidos pero no para ratones de 10 a 12 días, ratones adultos ni cobayos, aun a la más baja dilución inoculada. Los cobayos inoculados con las cepas recuperadas de humanos y con la cepa Candid#1 no presentaron síntomas de enfermedad y mostraron estar protegidos cuando fueron desafiados con una cepa patógena. Los índices de infección y de protección hallados indican que estas cepas poseen elevada capacidad infectante y protectora en las especies animales aquí estudiadas. Estos resultados demuestran que las cepas de virus Junín aisladas de voluntarios inmunizados con Candid#1 mantienen el mismo fenotipo atenuado de la vacuna Candid#1 después de un pasaje por humanos.

  3. IDENTIFICACIÓN DE CEPAS NATIVAS CON POTENCIAL PARA OBTENCIÓN DE POLIHIDROXIALCANOATOS -(PHAsEN LODOS ACTIVADOS

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    ANA LORENA ARROYAVE R.

    Full Text Available Los Polihidroxialcanoatos son poliésteres naturales que se han convertido en una excelente alternativa para remplazar los plásticos convencionales. Son biocompatibles y biodegradables y en corto tiempo pueden ser reducidos a dióxido de carbono y agua. Son sintetizados intracelularmente por diferentes bacteriasy pueden ser obtenidos a partir de una gran variedad de sustratos, en los que se incluyen algunos desechos agroindustriales. Con el objetivo de identificar cepas nativas con potencial para la producción de Polihidroxialcanoatos se llevó a cabo una bioprospección en los lodos de la Planta deTratamiento de Aguas Residuales de San Fernando (Itagüí-Antioquia. Se aislaron en agar selectivo 19 colonias, de las cuales se seleccionaron las colonias con oxidasa positiva. Se realizó la activación y siembra de las bacteriasen medio mínimo de sales (MSM líquido, para establecer el porcentaje de acumulación de los Polihidroxialcanoatos.Las cepas LM-3D, LAR-4D, LAR-5B,LAR-5E, presentaron un porcentaje de acumulación de PHAs de 2, 10, 4, 3 %, respectivamente y la cepaLM-3F presentó un porcentaje de acumulación del 25%, con respecto a los otros aislados. Tras el análisis bioquímico se determinó que las cepas productoras de PHAs fueron Shingomonas paucemobilis, Aeromonas hydrophila y Aeromonas sobria.

  4. EVALUACIÓN DE LA PRODUCCIÓN DE ETANOL A PARTIR DE MELAZA CON CEPAS NATIVAS Saccharomyces cerevisiae

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    FRANCISCO EMILIO ARGOTE V

    Full Text Available Este trabajo tuvo como objetivo principal evaluar la producción de etanol de nueve levaduras aisladas y caracterizadas en el Valle con un testigo de un ingenio brasilero. La evaluación de las variables como temperatura, oxígeno, dióxido de carbono y etanol durante el proceso de fermentación se realizó con la ayuda del equipo Ecochamber y la técnica HPLC para contrastar los valores encontrados. El diseño experimental correspondió al modelo unifactorial, y el tiempo de observación fue transversal en 48 horas, la unidad muestral fue de 600 mL de solución de melaza con una concentración de 16°Brix cuyo pH se ajustó a 4,5 con adición de acido cítrico. Los resultados se analizaron con el programa estadístico STATGRAPHICS 5.1, mediante ANOVA con un nivel de significancia de 0,05 y las diferencias con prueba de Tuckey. Como resultados relevantes se encontró que las variables temperatura y oxígeno no presentaron diferencias estadísticas con valores entre 24 a 32°C y 6,7 a 20% de manera respectiva, mientras que las cepas IC1, IP3, IP4, IP5 mostraron los mayores valores en producción de dióxido de carbono. En lo concerniente a la variable etanol, las cepas con mayor producción fueron T, IC1, IP3, IP4, IP6, IP5.

  5. POTENCIALIDADES DE Heterorhabditis bacteriophora Poinar CEPA HC1 PARA EL MANEJO DEHypothenemus hampei Ferr . II. COMPATIBILIDAD CON Beauveria bassiana (Balsamo ) Vuillemin y Endosulfan

    OpenAIRE

    Sánchez, Lourdes; Rodríguez, MayraG

    2008-01-01

    Heterorhabditis bacteriophora cepa HC1 ha demostrado ser un efectivo agente de control biológico para importantes plagas en las condiciones de Cuba, mostrando también potencialidades para el manejo de la broca del café. Con los objetivos de estudiar el tipo de relaciones que puede establecer con Beauveria bassiana , organismo de amplio uso a escala internacional para el manejo de la broca en condiciones de producción y el insecticida Endosulfan , se ejecutó este trabajo. H. bacteriophora cepa...

  6. Frecuencia de genes de virulencia en infecciones mixtas con cepas de Helicobacter pylori de una población mexicana

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    R. González-Vázquez

    2016-01-01

    Conclusiones: La prueba de Fisher no mostró una asociación significativa entre el resultado clínico y el genotipo en la población estudiada. Los genotipos circulantes en la población mexicana fueron cagA+, vacAs1, vacAm1. La PCR multiplex puede usarse para genotipificar rápidamente las cepas de H. pylori. cagE es un buen marcador para identificar cepas cag-PAI+.

  7. Caracterización genotípica y fenotípica de cepas emergentes de Escherichia coli aisladas de niños con diarrea en Bucaramanga y su área metropolitana, Colombia

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    Monica Yurley Arias Guerrero

    2016-06-01

    Full Text Available Introducción: La enfermedad diarreica aguda (EDA sigue siendo uno de los principales problemas en salud pública en países en desarrollo. Dentro de los agentes infecciosos que causan esta enfermedad se encuentran bacterias, virus y parásitos. Los agentes bacterianos ocupan un segundo lugar destacándose Escherichia coli enteropatógenas entre las más importantes. Actualmente, seis cepas con diferentes mecanismos de patogenicidad están asociados a diarrea  E. coli enteropatógena (ECEP, productora de toxina-shiga (ECTS, enterotoxigénica (ECET, enteroinvasiva (ECEI, enteroagregativa (ECEA y adherente difusa (ECAD, cada una con un set específico de genes de virulencia. No obstante, se han reportado brotes de EDA ocasionados por cepas de E. coli diferentes a los patotipos conocidos, denominadas cepas emergentes. Estos brotes han sido reportados en Alemania, Europa y recientemente en Cartagena Colombia, lo que sugiere que en nuestro medio circulan estas cepas. Por lo cual, es necesario caracterizar estas cepas emergentes. Objetivo: Caracterizar genotípicamente y fenotípicamente dos cepas emergentes de E. coli aisladas de muestras diarreicas en niños menores de cinco años en Bucaramanga, Colombia. Materiales y métodos: De un estudio de casos y controles realizado entre Julio del 2013 y diciembre del 2014 en Bucaramanga y su área metropolitana, Colombia a 860 niños menores de 5 años con diarrea (casos y sin diarrea (controles. Se aislaron a partir de coprocultivos cepas de E. coli mediante pruebas bioquímicas. A estas cepas previamente almacenadas a -80°C se les determino mediante PCR multiplex los patotipos de E. coli, los cuales posteriormente fueron confirmados mediante PCR simple. Muestras positivas para dos patotipos u otra característica fueron clasificadas como cepas emergentes. Finalmente, estas cepas se caracterizaron fenotípica y genotípicamente. Para ello, se llevaron a cabo pruebas como secuenciación de genes de

  8. Estudios sobre leishmaniasis tegumentaria en el Perú. I. Infección experimental de perros con cepas de leishmanias procedentes de casos de Uta

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    Arístides Herrer

    1951-01-01

    Full Text Available Se han inoculado varias series de perros con cepas de leishmanias procedentes de la forma clínica de la leishmaniasis tegumentaria conocida en el Perú con el nombre de uta, usando con tal objeto material tomado directamente de las lesiones utosas así como también cultivos del parásito. Los principales resultados obtenidos en tales inoculaciones son los siguientes: 1. Ha sido imposible infectar al perro con cultivos mantenidos solamente in vitro por espacio de cinco a ocho años, no obstante de haberse ensayado en tres series distintas de inoculaciones. 2. Se ha conseguido infecciones experimentales en el 87 por ciento de los perros que fueron inoculados con cultivos de reciente aislamiento. Aunque las inoculaciones fueran hechas bajo las mismas circunstancias en el dorso del hocico y la cara interna de una de las orejas, la infección se ha obtenido principalmente en el hocico. 3. También se ha logrado infectar perros inoculándoles material tomado directamente de las lesiones leishmaniásicas, ya sea de casos de uta o de lesiones producidas experimentalmente en otros perros.

  9. Identificación de los Genes cry en Cepas Mexicanas de Bacillus thuringiensis con Potencial Insecticida Identificación de los Genes cry en Cepas Mexicanas de Bacillus thuringiensis con Potencial Insecticida

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    J. E. Ibarra

    2012-02-01

    Full Text Available En el presente estudio se determinó la morfología y la composición proteica de los cristales de las cepas LBIT-499 y LBIT-504 de B. thuringiensis, ambas nativas de Guanajuato, México. La primera mostró sólo cristales bipiramidales mientras que la segunda presentó cristales tanto bipiramidales como cúbicos. Estos cristales estuvieron compuestos de proteínas de aproximadamente 130 y 60 kDa, respectivamente. En ambas cepas, además de la LBIT-500 y LBIT-544, se detectó una gran variedad de genes cry1. En éstas últimas se encontraron los genes cry2A y cry2B, en la LBIT- 504 sólo el cry2B y en la LBIT-499 no se detectó ningún cry2. This report revealed the crystal morphology and protein composition of strains LBIT-499 and LBIT-504 of B. thuringiensis, native to Guanajuato, Mexico. LBIT-499 showed only bipyramidal crystals, while LBIT-504 showed both bipyramidal and cubical crystals. These crystals were composed by proteins of ca. 130 and 60 kDa, respectively. Both strains as well as LBIT-500 and LBIT-544, showed a great variety of cry1 genes, while cry2A and cry2B were identified in LBT-500 and LBIT-544, LBIT-504 showed only the cry2B gene, and no cry2 was detected in LBIT-499.

  10. Aislamiento de tres cepas de leishmania

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    Florentino Rey

    1942-08-01

    Full Text Available Con el ánimo de estudiar el problema parasitológico de la Leishmaniasis cutánea en Colombia, emprendimos algunas experiencias al respecto. Este es un estudio de aislamiento de tres cepas de Leishmania obtenidas de tres enfermos de distintas regiones del país, Investigadores colombianos que se han ocupado con anterioridad de este problema, obtuvieron siempre resultados negativos. Comunicaciones posteriores informarán sobre estudios experimentales de las cepas mencionadas.

  11. Vegetal waste degradation by microbial strains inoculation Degradación de residuos vegetales mediante inoculación con cepas microbianas

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    Nubia Grijalva Vallejos

    2013-06-01

    degradadores demateriales lignocelulósicos por su capacidad de sintetizar enzimas extracelulares hidrolíticas yoxidativas en altas cantidades. Trichoderma reesei, Aspergillus niger, Penicillium sp. yPhanerochaete chrysosporium son cepas modelo cuya eficiencia de degradación de materialesricos en lignocelulosa incluso en presencia de contaminantes ha sido comprobada. Se estánrealizando varios estudios de mutagénesis dirigida, co-cultivo y expresión heteróloga, con elobjeto de mejorar el contenido de enzimas (celulasas, xylanasas, y β-glucosidasas en variascepas, además de nuevas búsquedas genéticas para encontrar otros microorganismos coneste potencial. Su principal aplicación es la producción industrial de etanol y metabolitossecundarios bajo condiciones controladas en procesos de fermentación. Esta revisiónproporciona una perspectiva general de las estrategias y metodologías actualmente usadaspara el aprovechamiento de residuos vegetales mediante inoculación de cepas microbianas.

  12. OBTENCIÓN DE DEXTRANO Y FRUCTOSA, UTILIZANDO RESIDUOS AGROINDUSTRIALES CON LA CEPA Leuconostoc mesenteroides NRRL B512-F

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    Olga Viviana Rodríguez

    2007-06-01

    Full Text Available Debido al gran potencial biotecnológico que presentan los residuos agroindustriales, se desarrolló el presente trabajo para la obtención de oligosacáridos, utilizando como materia prima residuos de cascaras de naranja, piña y cachaza de caña panelera, a escala de laboratorio. Por medio de un diseño experimental se evaluó la concentración y tipo de sustrato y la temperatura del proceso con tres niveles, para lograr la mayor producción. El desarrollo experimental se llevó a cabo con un volumen de 100 mL y 250 mL. En la etapa final se obtuvo como resultado una producción de 3,4 g/L de dextrano y 5,04 g/L de fructosa, utilizando como sustrato cascaras de naranja con estas condiciones: temperatura de 30 °C y concentración de sustrato de 20 g/L; durante el proceso se midieron el consumo de sustrato y la concentración de biomasa y productos. Se observó el desarrollo del microorganismo con los sustratos empleados en la experimentación, sin adición de nutrientes, con una adaptación favorable a éstos. Finalmente, se realizó una caracterización preliminar del polímero obtenido, con lo que se concluyó que puede obtenerse dextrano de grado técnico para su uso como espesante en la industria de alimentos y en el área de tratamiento de aguas residuales como floculante.Due to biotechnological potential that presents the agro-industrial remainders, the present work was developed about obtaining oligosaccharides using like raw material remainders of orange peels, pineapple peels, and panelera cane slowness, at level laboratory. An experimental design was used to evaluate type and concentration of substrate and process temperature at three levels, accordingly the greatest amount of dextran and fructose produced. All volumes worked were 100 mL and 250 mL. In the final phase, the result was a production of dextran 3.4 g/L and fructose 5.04 g/L, using orange peels as substrate, temperature at 30 °C and concentration of substrate of 20 g

  13. Una breve descripción de la vegetación, con especial énfasis en las pioneras intermedias de los bosques secos de la Jagua, en la cuenca alta del Rio Magdalena en el Huila

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    William Gerardo Vargas

    2015-01-01

    Full Text Available Se revisó la información existente sobre la flora de los bosques secos del sur del departamento del Huila, con especial interés en la de La Jagua y sus alrededores con el fin de identificar especies pioneras intermedias que puedan apoyar procesos de restauración ecológica y conservación de la biodiversidad. Para los bosques secos del departamento se identificaron 203 especies pioneras intermedias distribuidas en 123 géneros y 45 familias botánicas, mientras que para La Jagua 153 especies agrupadas en 109 géneros y 42 familias, entre las que sobresalen Fabaceae,  Rubiaceae, Malvaceae, Moraceae y Anacardiaceae. Se proponen 85 especies consideradas claves para procesos de conservación, la restauración de los ecosistemas secos de la región dirigida a la generación de hábitat y de conectividad, la producción de recursos para la fauna, así como la producción de leña y madera para las comunidades dentro de un modelo de herramientas de manejo del paisaje.

  14. Control biológico de la agalla de corona en plantas de crisantemo con la cepa k-84 de a Grobacterium Radiobacter var radiobacter (Beijerinck & Van delden Conn

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    Garzón Gerardo

    1986-12-01

    Full Text Available A partir de plantas de crisantemo de las variedades Surf, Yellow Garland y Clrcus afectadas por la agalla de corona en la Sabana de Bogotá, se obtuvieron 3 aislamientos bacterlales de A. radlobacter varo tumefaclens Smith &.. Townsend, los cuales se caracterizaron e identificaron mediante observaciones macroscópicas y microscópicas y la realización de pruebas fisiológicas y bioqurmicas en el laboratorio; para probar la patogenecidad de dichos aislamientos se inocularon en plantas de tomate, en discos de zanahoria y de remolacha.
    Se realizaron 2 ensayos para determinar el efecto de la cepa K-84 de A. radlobacter varo radlobacter sobre la enfermedad ocasionada por 2 aislamientos patógenos de A. radlobacter
    var. tumefaclens en plantas de crisantemo o de las variedades Clrcus, Yellow Garland y Surf. El primer ensayo se desarrollo en bancos de enraizamiento con esquejes sin enraizar y el segundo ensayo se hizo con esquejes enraizados y colocados en materas. Los 3 aislamientos patógenos presentaron un alto porcentaje de Infección en las variedades Circus y Yellow Garland, mientras que la variedad Surf fue la menos afectada. La cepa K-84 fue más efectiva en el ensayo de materas que en el ensayo en bancos de
    enraizamiento por la fácil lixiviación de la bacteria con el agua de riego. Se encontró un mejor control de la cepa K-84 para los aislamientos patógenos pertenecientes al blotlpo 2. La inmersión en preslembra de los esquejes en una solución de cloro y el uso continuo de este elemento en el agua de riego fueron el mejor tratamiento para .el control de la agalla de corona en bancos de enraizamiento yen materas.

  15. Lesión celular del miocardio y actividad de la ATPsintasa mitocondrial en ratas infectadas con una cepa colombiana de Trypanosoma cruzi

    OpenAIRE

    Dairo Alonso Rendón; Carlos M. Genes; Omar Triana

    2007-01-01

    Introducción. La enfermedad de Chagas es la principal causa de cardiomiopatía crónica en regiones endémicas de Latinoamérica. Alteraciones en el metabolismo energético mitocondrial cardiaco causadas por Trypanosoma cruzi pueden estar involucradas en el desarrollo de esta cardiomiopatía. Objetivo. En este trabajo se investigó la lesión celular del miocardio de ratas durante una infección por la cepa colombiana Mg8 de Trypanosoma cruzi y la actividad de la ATPsintasa mitocondrial para correl...

  16. Lesión celular del miocardio y actividad de la ATPsintasa mitocondrial en ratas infectadas con una cepa colombiana de Trypanosoma cruzi

    OpenAIRE

    Rendón, Dairo Alonso; Genes, Carlos M; Triana, Omar

    2007-01-01

    Introducción. La enfermedad de Chagas es la principal causa de cardiomiopatía crónica en regiones endémicas de Latinoamérica. Alteraciones en el metabolismo energético mitocondrial cardiaco causadas por Trypanosoma cruzi pueden estar involucradas en el desarrollo de esta cardiomiopatía. Objetivo. En este trabajo se investigó la lesión celular del miocardio de ratas durante una infección por la cepa colombiana Mg8 de Trypanosoma cruzi y la actividad de la ATPsintasa mitocondrial para correlaci...

  17. The Educational Uses of Intermedia.

    Science.gov (United States)

    Launhardt, Julie; Kahn, Paul

    1992-01-01

    Uses of Intermedia, computer software designed to help instructors express relationships between concepts in the sciences and humanities, are discussed. The kinds of educational problems Intermedia was intended to address are described, some materials created using it are surveyed, and experiences with Intermedia in various educational contexts…

  18. Análisis genómico comparativo de cepas peruanas de Mycobacterium tuberculosis

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    David Tarazona

    Full Text Available Objetivos. Analizar comparativamente tres secuencias genómicas de Mycobacterium tuberculosis(MTB: INS-SEN,cepa sensible; INS-MDR, cepa multidrogorresistente e INS-XDR, cepa extensamente resistente, procedentes de la Ciudad de Lima, Perú. Materiales y métodos. Se identificaron los polimorfismos de un solo nucleótido (SNPs específicos en las cepas INS-SEN, INS-MDR y INS-XDR mediante el criterio de inclusión/exclusión. Se compararon los tres genomas de MTB y se construyó una filogenia molecular con 27 cepas de MTB de otros estudios, disponibles de la base de datos Genbank. Los SNPs específicos en cada genoma fueron organizados en clústers de grupos ortólogos (COGs. Resultados. El análisis de genomas permitió identificar un conjunto de SNPs asociados a determinantes de virulencia (familia de proteínas mce, policetidos, phiRv1, transposasas, metiltransferasas y relacionados a síntesis de vitaminas principalmente. Se observa una estrecha relación entre la cepa INS-MDR y INS-XDR, con solo un 6,1% de SNPs diferentes, sin embargo, la cepa INS-SEN presenta un 50,2 y 50,3% de SNPs diferentes a las cepas MDR y XDR, respectivamente. La filogenia molecular agrupó a las cepas peruanas dentro del linaje LAM y cercanamente a las cepas F11 y KZN de Sudáfrica. Conclusiones. Se evidenció una alta similitud (99,9% de la cepa INS-SEN con la cepa sudafricana F11, de gran alcance mundial, mientras los análisis de las cepas INS-MDR e INS-XDR demuestran una probable expansión de la familia KZN, cepa de Sudáfrica con alta virulencia y patogenicidad.

  19. The feasibility of using antigens prepared with rough Brucella strains for diagnosis of canine brucellosis Utilidad de los antígenos preparados con cepas rugosas de Brucella en el diagnóstico de brucelosis canina

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    G. I. Escobar

    2010-02-01

    Full Text Available Clinical diagnosis of canine brucellosis is not sensitive enough and a negative blood culture cannot rule out the disease. Indirect methods of serological testing such as agar gel immunodiffusion (AGID, rapid slide agglutination test (RSAT and indirect enzyme linked immunoassay (IELISA are preferred for routine diagnosis. Since Brucella canis shares antigenic components with the Brucella ovis and Brucella abortus RB51 strain, it would seem that either strain could be used as antigen. We present data on AGID and IELISA tests using the B. ovis antigen, RSAT and IELISA using the B. canis antigen and IELISA using the B. abortus RB51 antigen. The cut-off values were adjusted by the ROC analysis; the IELISA-B. ovis cut-off value was 23 (%P and the IELISA-B. abortus RB51, 24 (%P, with 100% sensitivity and 98.8% specificity. RSAT detected 100% of positive cases, while AGID was less sensitive. The sera from dogs treated with antibiotic showed that %P correlated well with the clinical course. Sera from dogs presumptively infected with B. suis were negative in all tests performed with the rough Brucella strains. RSAT is a very sensitive screening test and IELISA-B. canis, B. ovis and B. abortus RB51 could be used as confirmatory tests, since they show good specificity and sensitivity.Las técnicas más usadas en el diagnóstico de brucelosis canina son la inmunodifusión en gel de agar (AGID, la microaglutinación en portaobjetos (RSAT y el ELISA indirecto ya que el diagnóstico clínico es poco sensible y el bacteriológico no excluye la enfermedad. Como Brucella canis comparte componentes antigénicos con Brucella ovis y Brucella abortus RB51, estas cepas podrían ser usadas indistintamente como antígenos. En este trabajo presentamos datos sobre las pruebas de AGID e IELISA con antígeno B. ovis, RSAT e IELISA con antígeno B. canis e IELISA con antígeno B. abortus RB51. Los puntos de corte ajustados con la curva ROC fueron (%P 23 para IELISA-B. ovis y

  20. Histometría de la glándula sublingual de ratones (Mus musculus machos y hembras infectados con la cepa RAL del parásito de Chagas, Trypanosoma cruzi

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    Sérgio de Albuquerque

    2008-06-01

    Full Text Available Analizamos morfológica e histométricamente la glándula sublingual de ratones infectados con la cepa RAL del Trypanosoma cruzi, en machos y hembras. Usamos ratones albinos (Mus musculus, variedad Swiss, inoculados con 2x104 tripomastigotes sanguíneos de la cepa RAL del T. cruzi.. Sacrificamos los animales en el pico de la parasitemia (12º día y fijamos las glándulas sublinguales en ALFAC. Los cortes histológicos teñidos con HE fueron evaluados histométricamente (cariometría y estereología. La parasitemia fue más elevada en las hembras. Histopatológicamente, los "ácinos" (acini de los animales infectados eran menores, con escasa secreción, y conductos estriados menores. Los núcleos de las "medialunas" eran menores y había nidos de amastigotes en el citoplasma. Cariométricamente, los núcleos de los ácinos, medialunas y conductos estriados eran menores en los ratones infectados. Estereológicamente, los volúmenes relativos ocupados por ácinos y conductos estriados fueron menores e, inversamente, fue mayor el volumen para el tejido conjuntivo de los machos infectados. Las densidades de superficie de ácinos y conductos fueron mayores, y el diámetro y el espesor de la pared menores, en este grupo. Por otro lado, la densidad de ácinos fue menor, y las de los conductos estriados y tejido conjuntivo, fueron mayores en las hembras infectadas. Las densidades de superficie de ácinos y conductos estriados fueron mayores, mientras que el diámetro y espesor de la pared de los ácinos fueron menores (y las de los conductos estriados mayores, en este grupo. La cepa RAL del T. cruzi causó un cuadro general de atrofia general en la glándula sublingual, con numerosos nidos de parásitos.Histometry of the sublingual gland in male and female mice (Mus musculus infected with the RAL strain of the Chagas parasite, Trypanosoma cruzi. The aim of this work was to analyze histologically and histometrically the sublingual gland of mice infected

  1. Transformación de Agrobacterium tumefaciens CEPA LBA4404 con el vector binario pNOV022, selección in vitro y caracterización molecular

    Directory of Open Access Journals (Sweden)

    Janneth Fabiola Santos Rodríguez

    2002-01-01

    Full Text Available La caracterización molecular de la infección causada por Agrobacterium tumefaciensy el des-cubrimiento de que una parte del plásmido inductor de tumores (T-DNA es transferido algenoma de las plantas que infecta, han permitido utilizar esta bacteria como sistema detransformación vegetal. La utilización de esta tecnología ha permitido a los científicos resolverproblemas con relación a especies cultivables. En el presente trabajo se realizó la transfor-mación de las cepas LBA4404 de Agrobacterium tumefaciensy DH5a de Escherichia coli. Para tal finse utilizó la técnica de choque térmico incorporando el vector binario pNOV022, que contenía6868Resúmenes la construcción p35S-IP-t35S - pUBQ-PMI-tNos (promotor 35S del CaMV, inhibidor de protea-sas, terminador 35S del CaMV, promotor ubiquitina 3, fosfomanosa isomerasa y terminadorde la nopalinsintetasa. Igualmente se realizó selección de clones bacterianos transformados(basado en la resistencia a espectinomicina y/o estreptomicina y su caracterización molecularpor medio de PCR y un perfil de restricción. Este trabajo apuntó a producir un vector adecuadopara la transformación estable de papa criolla (Solanum phureja y de papa (Solanum tuberosum,un gen que confiere resistencia a insectos. Los resultados de las metodologías empleados evi-denciaron la introducción exitosa del plásmido recombinante en E. coliy A. tumefaciens. Si bienla eficiencia de transformación fue baja (0,032 x 10-8, los resultados de la PCR mostraron laamplificación de una secuencia de 500 pb del gen inhibidor de proteasa aislado a partir de lascepas transformadas. En cuanto al perfil de restricción los resultados no fueron los esperados,sin embargo, permiten diferenciar la cepa de A. tumefacienstransformada. Adicionalmente seestandarizó las condiciones de los medios de selección para diferenciar cepas transformadas.

  2. Ramalina intermedia in Nederland

    NARCIS (Netherlands)

    Segal, S.

    1968-01-01

    The lichen Ramalina intermedia Del. apud Lamy was found near Apeldoorn on a decayed wall of an old castle in the possession of the Royal family. The wall, rising up from the castle moat, dates from the 16th century, but remained covered with soil for several centuries after the moat had been filled

  3. Portadores de Neisseria meningitidis, caracterización de las cepas aisladas y respuesta inmune basal a VA-MENGOC-BC

    Directory of Open Access Journals (Sweden)

    María J. Valdés

    2008-08-01

    Full Text Available Con el propósito de conocer la prevalencia de portadores de Neisseria meningitidis en un grupo de adolescentes, los marcadores epidemiológicos de las cepas aisladas, así como los factores de riesgo asociados con el estado de portador y la respuesta inmune basal a VA-MENGOC-BC , se realizó un estudio transversal descriptivo de portadores en 189 estudiantes de 12-19 años de un politécnico de Ciego de Ávila, siguiendo las Normas Bioéticas establecidas. A los estudiantes se les realizó un exudado faríngeo y una extracción de sangre para la obtención de suero, así como una encuesta relacionada con aspectos de la investigación. La identificación de N. meningitidis se hizo por el sistema API NH (bioMérieux. Los serosubtipos e inmunotipos se clasificaron por ELISA de células enteras con anticuerpos monoclonales y la respuesta inmune basal se detectó por el Ensayo Bactericida del Suero. Se determinó la susceptibilidad antimicrobiana de las cepas frente a la penicilina, cloranfenicol, rifampicina, sulfadiacina sódica, ceftriaxona y ciprofloxacina. La prevalencia de portadores de N. meningitidis fue del 17%. Predominaron las cepas no agrupables (84,7%, seguidas por los serogrupos B (12,5% y Z (3,1%, destacándose la ausencia del C. Prevaleció el fenotipo NA:NT:P1.NST:L3,7,9 (12,5%, las cepas resistentes a la sulfadiacina (78,2% y sensibles a penicilina (81,3%, aunque el 18,7% mostró sensibilidad intermedia a este fármaco. Al resto de los antimicrobianos todas fueron sensibles. Se constató una respuesta inmune de memoria a la vacuna antimeningocócica (VAMENGOC-BC®, 12 años después de su aplicación, con títulos bactericidas anti C y B de 25 y 42%, respectivamente, resultados que pudieran estar influenciados por la inmunización sistemática que se realiza en Cuba con esta vacuna desde 1991.

  4. Validación de un ELISA para la cuantificación de las impurezas proteicas de la cepa hospedera en el principio activo de la vacuna recombinante cubana contra la hepatitis B

    Directory of Open Access Journals (Sweden)

    Eliana Pérez

    2014-04-01

    Full Text Available Se validó un inmunoensayo tipo sandwich de doble anticuerpo para cuantificar las impurezas proteicas de la cepa hospedera que pueden estar presentes en el principio activo de la vacuna cubana contra la hepatitis B. Se prepararon los reactivos biológicos empleados en el ELISA. Las proteínas de la cepa hospedera se obtuvieron bajo las mismas condiciones que el proceso de producción de la proteína recombinante hasta un paso de semipurificación o purificación primaria. Los antisueros dirigidos contra las proteínas de la cepa hospedera se generaron en conejos por un proceso de inmunización en cascada. Para la validación se analizaron los siguientes parámetros: linealidad, límite de detección y cuantificación, exactitud, rango y precisión. El ensayo establecido resultó específico, con una exactitud entre 89% y 109% para todos los tampones estudiados; se demostró un ajuste parabólico y un rango de trabajo entre 0,63 y 1,25 ng/mL, la repetibilidad y la precisión intermedia mostraron coeficientes de variación inferiores al 10 y 20% respectivamente.

  5. CARACTERIZACIÓN BIOLÓGICA DE VARIANTES DE PLACA DE LA CEPA VACUNAL 17D CONTRA LA FIEBRE AMARILLA

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    María Rojas

    2009-09-01

    Full Text Available La vacuna colombiana 17D, contiene por lo menos cuatro fenotipos, denominados pequeño (0.3 – 1.2 mm, mediano (1.3 – 2.1 mm, grande (2.2 – 3.0 mm y extragrande (>3.1 mm. La composición y distribución porcentual de esos fenotipos, varió entre lotes y entre ampolletas de un mismo lote. Cada variante fue clonada por dilución de la vacuna y su efecto virulento fue analizado en ratones; el fenotipo de placa pequeño estuvo ligeramente sub representado en los lotes analizados y mostró una virulencia similar a la de la cepa silvestre neurotrópica Francesa (LD50 > 10-6, mientras que el fenotipo predominante y mas atenuado fue el mediano (LD50: 10-4. Los fenotipos grande y extragrande mostraron una virulencia intermedia (LD 50: 10 – 5  con relación a los anteriores. Los análisis de secuencia de las variantes sobre una región comprendida entre el extremo 3´NS5 y el inicio de 3´NCR, mostró la cercanía entre aquellas variantes con algún grado de virulencia, y entre la variante atenuada y la vacuna colombiana. La heterogeneidad de la vacuna 17D, constituye una evidencia de la estructura de quasiespecies propia de los virus RNA y señala cómo los casos de reacciones post vacunales  adversas pueden estar asociados con la aplicación de vacunas fabricadas a partir de cepas virales atenuadas.  

  6. Patrones de adherencia de cepas de Escherichia coli Difusamente adherente (DAEC provenientes de niños con y sin diarrea Adhesion patterns in diffusely adherent Escherichia coli (DAEC strains isolated from children With and without diarrhea

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    Maribel Riveros

    2011-03-01

    Full Text Available Introducción. Las E. coli de adherencia difusa (DAEC son el sexto grupo de E. coli diarrogénicas reconocidas. Su asociación con diarrea es controversial. No se conoce la variabilidad en los patrones de adherencia de cepas clínicas. Objetivos. Comparar los patrones de adherencia entre cepas aisladas de niños con y sin diarrea. Materiales y métodos. Se analizó 31 cepas DAEC, 25 de diarrea y 6 de niños asintomáticos (control aislados de un estudio de cohorte de niños menores de 12 meses en el cono sur de Lima. Las DAEC fueron identificadas por PCR (gen daaD. Se evaluó el patrón y grado de adherencia en cultivos de células HEp-2; la polimerización de actina se evaluó por la prueba de coloración de fluorescencia de actina (FAS; y la motilidad se evaluó por métodos convencionales microbiológicos. Resultados. El patrón de adherencia difusa se encontró en el 88% de muestras de diarrea y en el 100% de muestras control. La cantidad de bacterias adheridas por célula fue significativamente menor en las muestras de diarrea (pIntroduction. Diffusely adherent E. coli (DAEC is the sixth recognized group of diarrheagenic E. coli. However, its association with diarrhea remains controversial. Variability in the adherence patterns of clinical strains is unknown. Objectives. To compare the adherence patterns between strains isolated from children with and without diarrhea. Materials and methods. A total of 31 DAEC strains were analyzed, 25 from children with diarrhea and 6 from asymptomatic (control children, isolated from a cohort study of children under one year of age in the southern districts of Lima. DAEC were identified by PCR (daaD gene. The pattern and adherence score in HEp-2 cell culture were evaluated, Actin polimerization was determined by fluorescence actin staining (FAS and motility was evaluated by conventional microbiology methods. Results. Diffuse adherence pattern was found in 88% of diarrhea samples and in the total of

  7. Asociación de cepas fijadoras de nitrógeno de vida libre, con recursos genéticos de pastos para zonas áridas.

    OpenAIRE

    Plascencia Jiménez, Raúl

    2014-01-01

    Para evaluar microorganismos de la rizosfera de Bouteloua curtipendula (Michx.) Torr., se recolectó suelo de cinco localidades 1) Salinas, San Luis Potosí 2) Ejido, Jacalón, Salinas, San Luis Potosí; 3) Calera, Zacatecas; 4) Santa María del Oro, Durango y 5) Crucero La Zarca, Durango. La población de actinomicetos, bacterias y hongos, se estimó con diluciones seriadas (10-1 a 10-10). Los actinomicetos (AS), bacterias (BS) y hongos (HS) se estimaron con diluciones 10-3 a 10-5; 10-5 a 10-7; 10-...

  8. AISLAMIENTO DE CEPAS DE ESCHERICHIA COLI DESDE CASOS CLÍNICOS DE INFECCIÓN VAGINAL: ASOCIACIÓN CON OTROS MICROORGANISMOS Y SUSCEPTIBILIDAD ANTIBACTERIANA

    OpenAIRE

    Padilla E, Carlos; Lobos G, Olga; Padilla E, Ramiro; Fuentes V, Leoncio; Núñez F, Loreto

    2007-01-01

    Objetivo: Determinar la presencia de Escheríchia coli en fluidos vaginales de mujeres con infección vaginal y analizar la susceptibilidad antimicrobiana. Método: Se estudiaron 425 muestras de mujeres con diagnóstico clínico de infección vaginal (casos) y 100 mujeres sanas (controles). Las muestras vaginales fueron estudiadas mediante los criterios de Amsel y Nugent. Se utilizaron diferentes metodologías para identificar: Trichomonas vaginalis, Candida albicans, Gardnerella vaginalis, Chlamydi...

  9. Circaea intermedia Ehrh. bij Winterswijk

    NARCIS (Netherlands)

    Weeda, E.J.

    1980-01-01

    Circaea intermedia, collected twice in southern Limburg (1897, 1939) and once near Winterswijk in the east of Gelderland (1940), was refound at the latter locality in 1979. The vegetation in which it occurs is shown by means of a relevé. The population of C. intermedia appears to form a clone, which

  10. [Current management of thalassemia intermedia].

    Science.gov (United States)

    Thuret, I

    2014-11-01

    Thalassemia intermedia is a clinical entity where anemia is mild or moderate, requiring no or occasional transfusion. Non-transfusion-dependent thalassemia encompasses 3 main clinical forms: beta-thalassemia intermedia, hemoglobin E/beta-thalassemia and alpha-thalassemia intermedia (HbH disease). Clinical severity of thalassemia intermedia increases with age, with more severe anemia and more frequent complications such as extramedullary hematopoiesis and iron overload mainly related to increased intestinal absorption. Numerous adverse events including pulmonary hypertension and hypercoagulability have been associated with splenectomy, often performed in thalassemia intermedia patients. The potential preventive benefit of transfusion and chelation therapies on the occurrence of numerous complications supports the strategy of an earlier therapeutic intervention. Increasing knowledge about pathophysiological mechanisms involved in thalassemia erythropoiesis and related iron overload is currently translating in novel therapeutic approaches. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  11. Evaluación del crecimiento y producción de biomasa de dos cepas del género Pleurotus spp., cultivadas en un medio agar con diferentes sustratos

    Directory of Open Access Journals (Sweden)

    Carol Daniela Coello-Loor

    2017-12-01

    Full Text Available La velocidad de crecimiento radial (VCR (mm.h-1 y la producción de biomasa (PB (g.g-1 de sustrato seco son técnicas que puedan establecer el grado de adaptación y desarrollo de los hongos del género Pleurotus spp., a distintos sustratos que podrían emplearse en una fermentación en medio sólido. Las especies fueron Pleurotus sapidus (Ps y Pleurotus ostreatus IE8 (Po. El medio de cultivo sintético empleado fue el papa dextrosa agar (PDA, con un pH que va de 5.6 a 5.9, ideal para el crecimiento de hongos, tiene todos los componentes nutritivos, y ligera acidez que logran la inhibición del desarrollo de bacterias; diluido en 4 diferentes soluciones preparadas con los materiales residuales (solución cascarilla de arroz CaPDA, solución cáscara de maracuyá CmPDA, solución mezcla 50% Cascarilla de arroz+50% Cáscara de maracuyá CaCmPDA y agua destilada+PDA con el propósito de observar el crecimiento radial cada 24 horas y la producción de biomasa fúngica de estos hongos lignocelulósicos por su periodo de incubación y la adaptación a nivel in vitro. Los tratamientos que presentaron mejor comportamiento VCR fueron el PoCaPDA (0.569 y el PoCaCmPDA (0.549; la cepa que reporto valores más altos en VCR y PB fue el Pleurotus ostreatus y el mejor medio de cultivo fue el CaPDA, en ambas variables; mientras, la mayor producción de biomasa fue en Pleurotus sapidus en CaPDA (0.1727 y el Pleurotus ostreatus IE8 en CmPDA (0.1722, CaPDA (0.1706 y PDA (0.1694.

  12. THALASSAEMIA INTERMEDIA : AN UPDATE

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    Ali Taher

    2009-08-01

    Full Text Available Our understanding of the molecular and pathophysiological mechanisms underlying the disease process in patients with thalassaemia intermedia (TI has substantially increased over the past decade. TI encompasses a wide clinical spectrum of beta-thalassaemia phenotypes. Some TI patients are asymptomatic until adult life, whereas others are symptomatic from as young as 2 years of age. A number of clinical complications commonly associated with TI are rarely seen in thalassaemia major, including extramedullary hematopoiesis, leg ulcers, gallstones, thrombosis and pulmonary hypertension. There are a number of options currently available for managing patients with TI, including transfusion therapy, iron chelation therapy, modulation of foetal haemoglobin production and haematopoietic stem cell transplantation. However, at present, there are no clear guidelines for an orchestrated optimal treatment plan.

  13. THALASSAEMIA INTERMEDIA : AN UPDATE

    Directory of Open Access Journals (Sweden)

    Maria Domenica Cappellini

    2009-06-01

    Full Text Available

    Our understanding of the molecular and pathophysiological mechanisms underlying the disease process in patients with thalassaemia intermedia (TI has substantially increased over the past decade. TI encompasses a wide clinical spectrum of beta-thalassaemia phenotypes. Some TI patients are asymptomatic until adult life, whereas others are symptomatic from as young as 2 years of age. A number of clinical complications commonly associated with TI are rarely seen in thalassaemia major, including extramedullary hematopoiesis, leg ulcers, gallstones, thrombosis and pulmonary hypertension. There are a number of options currently available for managing patients with TI, including transfusion therapy, iron chelation therapy, modulation of foetal haemoglobin production and haematopoietic stem cell transplantation. However, at present, there are no clear guidelines for an orchestrated optimal treatment plan.

  14. [Ecology suitability study of Ephedra intermedia].

    Science.gov (United States)

    Ma, Xiao-Hui; Lu, You-Yuan; Huang, De-Dong; Zhu, Tian-Tian; Lv, Pei-Lin; Jin, Ling

    2017-06-01

    The study aims at predicting ecological suitability of Ephedra intermedia in China by using maximum entropy Maxent model combined with GIS, and finding the main ecological factors affecting the distribution of E. intermedia suitability in appropriate growth area. Thirty-eight collected samples of E. intermedia and E. intermedia and 116 distribution information from CVH information using ArcGIS technology were analyzed. MaxEnt model was applied to forecast the E. intermedia in our country's ecology. E. intermedia MaxEnt ROC curve model training data and testing data sets the AUC value was 0.986 and 0.958, respectively, which were greater than 0.9, tending to be 1.The calculated E. intermedia habitat suitability by the model showed a high accuracy and credibility, which indicated that MaxEnt model could well predict the potential distribution area of E. intermedia in China. Copyright© by the Chinese Pharmaceutical Association.

  15. Fenoloxidasa Modificada: Clave para identificar cepas de Cryptococcus neoformans

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    Carlos Canelo D

    2000-01-01

    Full Text Available Cryptococcus neoformans es la única levadura patógena capaz de sintetizar pigmentos como la melanina mediante la actividad de su enzima llamada fenoloxidasa. El objetivo del presente estudio fue implementar y estandarizar la prueba de la fenoloxidasa, como técnica complementaria en la identificación de cepas de C. neoformans. Se estudiaron 21 cepas, identificadas previamente con métodos convencionales. La prueba de la fenoloxidasa fue modificada debido a que su empleo originaba 9,6% (2/21 de falsa negatividad. Esta prueba modificada se optimizó a 28°C a partir de un medio con baja concentración de glucosa. Ningún aislamiento falso negativo fue encontrado luego de repetir tres veces el ensayo, y el pigmento melanina fue detectado con mayor rapidez.

  16. Aislamiento e identificaci?n de diez cepas bacterianas desnitrificantes a partir de un suelo agr?cola contaminado con abonos nitrogenados proveniente de una finca productora de cebolla en la Laguna de Tota, Boyac?, Colombia

    OpenAIRE

    Ostos Ortiz, Olga Lucia; Benavides L?pez de Mesa, Joaqu?n; Quintero, Gladys

    2006-01-01

    Este articulo presenta los resultados de la versatilidad adaptativa y tolerancia de una consorcio bacteriano constituido por 10 cepas bacterianas end?micas de un suelo agr?cola contaminado, proveniente de una finca productora de cebolla ubicada en la laguna de Tota en Aquitania, Boyac?, Colombia, expuesto al uso incontrolado de fertilizantes nitrogenados. La consorcio bacteriano aislado del suelo est? conformado por: Bacillus megaterium, Bacillus licheniformis, Pseudomonas stutzeri, Acinetoba...

  17. EVALUACIÓN DE LA PRODUCCIÓN DE ETANOL UTILIZANDO CEPAS RECOMBINANTES DE Saccharomyces cerevisiae A PARTIR DE MELAZA DE CAÑA DE AZÚCAR

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    CAROLINA PEÑA

    2009-01-01

    Full Text Available Se evaluó la producción de etanol, el crecimiento celular y el consumo de sustrato de tres cepas de Saccharomyces cerevisiae: CBS8066 (control y dos recombinantes desarrolladas en la Unidad de Biotecnología Vegetal de CIB, GG570-CIBI y GG570-CIBII. Dichas cepas estuvieron bajo el efecto de dos concentraciones de sacarosa (170 y 250g/L y dos sustratos (industrial con melaza caña azúcar y sintético con sacarosa. Durante la fermentación en sustrato industrial se obtuvo mayor producción de etanol a concentración de 250g sacarosa/L. Bajo estas condiciones, la cepa GG570-CIBII produjo en promedio 2,34g etanol/L mas con respecto a la cepa control y en adición, a las 10h, produjo 8,02g/L por encima de la cepa control. Por otro lado, la cepa GG570- CIBI produjo 3,46g etanol/L menos que la cepa control. De esta forma, se encontró que la cepa GG570-CIBII es tolerante a alta concentración de sacarosa, y además, es capaz de producir una concentración mayor de etanol con respecto a la cepa control en melaza caña azúcar con 250g/L sacarosa.

  18. β-Thalassemia Intermedia: A Bird’s-Eye View

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    Anthony Haddad

    2014-03-01

    Full Text Available Beta-thalassemia is due to a defect in the synthesis of the beta-globin chains, leading to alpha/beta imbalance, ineffective erythropoiesis, and chronic anemia. The spectrum of thalassemias is wide, with one end comprising thalassemia minor, which consists of a mild hypochromic microcytic anemia with no obvious clinical manifestations, while on the other end is thalassemia major, characterized by patients who present in their first years of life with profound anemia and regular transfusion requirements for survival. Along the spectrum lies thalassemia intermedia, a term developed to describe patients with manifestations that are neither mild enough nor severe enough to be classified in the spectrum’s extremes. Over the past decade, our understanding of β-thalassemia intermedia has increased tremendously with regards to molecular information as well as pathophysiology. It is now clear that β-thalassemia intermedia has a clinical presentation as well as complications associated with the disease that are different from those of β-thalassemia major. This review is designed to tackle issues related to β-thalassemia intermedia from the basic definition of the disease to paramedical issues, namely the quality of life in these patients. Genetics and pathophysiology are revisited, as well as the complications specific to this disease. These complications include effects on several organ systems, including the cardiovascular, hepatic, endocrine, renal, brain, and skeletal systems. Extramedullary hematopoiesis is also discussed in this article. Risk factors are highlighted and cutoffs are identified to minimize morbidities in β-thalassemia intermedia. Several treatment modalities are considered by shining a light on the pros and cons of each modality, as well as the role of special pharmacological agents in the progress of the disease and its morbidities. Finally, health-related quality of life is discussed in these patients with a direct comparison

  19. β-Thalassemia Intermedia: A Bird’s-Eye View

    Science.gov (United States)

    Haddad, Anthony; Tyan, Paul; Radwan, Amr; Mallat, Naji; Taher, Ali

    2014-01-01

    Beta-thalassemia is due to a defect in the synthesis of the beta-globin chains, leading to alpha/beta imbalance, ineffective erythropoiesis, and chronic anemia. The spectrum of thalassemias is wide, with one end comprising thalassemia minor, which consists of a mild hypochromic microcytic anemia with no obvious clinical manifestations, while on the other end is thalassemia major, characterized by patients who present in their first years of life with profound anemia and regular transfusion requirements for survival. Along the spectrum lies thalassemia intermedia, a term developed to describe patients with manifestations that are neither mild enough nor severe enough to be classified in the spectrum’s extremes. Over the past decade, our understanding of β-thalassemia intermedia has increased tremendously with regards to molecular information as well as pathophysiology. It is now clear that β-thalassemia intermedia has a clinical presentation as well as complications associated with the disease that are different from those of β-thalassemia major. This review is designed to tackle issues related to β-thalassemia intermedia from the basic definition of the disease to paramedical issues, namely the quality of life in these patients. Genetics and pathophysiology are revisited, as well as the complications specific to this disease. These complications include effects on several organ systems, including the cardiovascular, hepatic, endocrine, renal, brain, and skeletal systems. Extramedullary hematopoiesis is also discussed in this article. Risk factors are highlighted and cutoffs are identified to minimize morbidities in β-thalassemia intermedia. Several treatment modalities are considered by shining a light on the pros and cons of each modality, as well as the role of special pharmacological agents in the progress of the disease and its morbidities. Finally, health-related quality of life is discussed in these patients with a direct comparison to the more severe

  20. β-Thalassemia Intermedia: A Bird's-Eye View.

    Science.gov (United States)

    Haddad, Anthony; Tyan, Paul; Radwan, Amr; Mallat, Naji; Taher, Ali

    2014-03-01

    Beta-thalassemia is due to a defect in the synthesis of the beta-globin chains, leading to alpha/beta imbalance, ineffective erythropoiesis, and chronic anemia. The spectrum of thalassemias is wide, with one end comprising thalassemia minor, which consists of a mild hypochromic microcytic anemia with no obvious clinical manifestations, while on the other end is thalassemia major, characterized by patients who present in their first years of life with profound anemia and regular transfusion requirements for survival. Along the spectrum lies thalassemia intermedia, a term developed to describe patients with manifestations that are neither mild enough nor severe enough to be classified in the spectrum's extremes. Over the past decade, our understanding of β-thalassemia intermedia has increased tremendously with regards to molecular information as well as pathophysiology. It is now clear that β-thalassemia intermedia has a clinical presentation as well as complications associated with the disease that are different from those of β-thalassemia major. This review is designed to tackle issues related to β-thalassemia intermedia from the basic definition of the disease to paramedical issues, namely the quality of life in these patients. Genetics and pathophysiology are revisited, as well as the complications specific to this disease. These complications include effects on several organ systems, including the cardiovascular, hepatic, endocrine, renal, brain, and skeletal systems. Extramedullary hematopoiesis is also discussed in this article. Risk factors are highlighted and cutoffs are identified to minimize morbidities in β-thalassemia intermedia. Several treatment modalities are considered by shining a light on the pros and cons of each modality, as well as the role of special pharmacological agents in the progress of the disease and its morbidities. Finally, health-related quality of life is discussed in these patients with a direct comparison to the more severe

  1. Sensibilidad al benzonidazol de cepas de Trypanosoma cruzi sugiere la circulación de cepas naturalmente resistentes en Colombia

    Directory of Open Access Journals (Sweden)

    Ana María Mejía-Jaramillo

    2012-06-01

    Full Text Available Introducción. La enfermedad de Chagas, causada por Trypanosoma cruzi, es uno de los problemas más graves de salud pública en el continente americano. El benzonidazol es uno de los dos medicamentos utilizados para tratar la enfermedad de Chagas. Sin embargo, la variación de la sensibilidad del parásito a este medicamento es una de las principales causas del fracaso del tratamiento. Objetivo. Evaluar la sensibilidad in vitro al benzonidazol de cepas colombianas de T. cruzi de diferentes orígenes y procedencia geográfica. Materiales y métodos. Treinta y tres cepas colombianas de T. cruzi aisladas de humanos, vectores y mamíferos, se analizaron in vitro mediante el micrométodo enzimático de MTT para determinar la concentración inhibitoria 50 (CI50 al benzonidazol. Se estudió la correlación entre la sensibilidad in vitro al medicamento y diferentes parámetros biológicos y eco-epidemiológicos. Resultados. El análisis de sensibilidad al medicamento indicó que el 36 % de las cepas eran sensibles, el 48 %, parcialmente resistentes y, el 16 %, resistentes al benzonidazol. Los análisis de correlación entre las CI50 con algunos parámetros biológicos y eco-epidemiológicos, mostraron diferencias en cuanto a la sensibilidad según el origen biológico y el área geográfica de procedencia de la cepa. Conclusiones. Existe una gran variabilidad en cuanto a la sensibilidad al benzonidazol de las cepas circulantes de T. cruzi en Colombia, lo cual sugiere la presencia de cepas naturalmente resistentes en el país.   doi: http://dx.doi.org/10.7705/biomedica.v32i2.458

  2. Diagnostic accuracy and therapeutic impact of endoscopic ultrasonography in patients with intermediate suspicion of choledocholithiasis and absence of findings in magnetic resonance cholangiography Precisión diagnóstica e impacto terapéutico de la ultrasonografía endoscópica en los pacientes con sospecha intermedia de coledocolitiasis y colangiorresonancia normal

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    Enrique Vázquez-Sequeiros

    2011-09-01

    Full Text Available Background: endoscopic ultrasonography (EUS and magnetic resonance cholangiography (MRC are the elective tests in the diagnosis of choledocholithiasis. MRC is best accepted by patients, but its sensitivity might decrease in the evaluation of microlithiasis. Aim: to evaluate the diagnostic accuracy and therapeutic impact of EUS in a prospective cohort of patients with intermediate suspicion of choledocolithiasis and no findings in MRC (normal MRC. Material and methods: during a period of 24 months, all the patients with clinical intermediate suspicion of choledocholithiasis and normal MRC were included. Sensitivity, specificity, diagnostic accuracy, positive predictive value (PPV and negative predictive value (NPV of MRC and EUS were compared, and so their impact in the management of these patients. Results: seventy six patients were evaluated (lithiasis in 30% of them. Sensitivity and diagnostic accuracy of EUS (100%, 92% were significantly higher than MRC values (0%, 70% (p Antecedentes: la ultrasonografía endoscópica (USE y la colangiografía por resonancia magnética nuclear (CRM son las técnicas de elección para el diagnóstico de litiasis en la vía biliar. Aunque la CRM es mejor aceptada por los pacientes, la sensibilidad de esta para detectar litiasis de pequeño tamaño podría ser insuficiente. Objetivo: estudiar de forma prospectiva la precisión diagnóstica y el impacto terapéutico de la USE en los pacientes con sospecha intermedia de coledocolitiasis y CRM normal. Material y métodos: se incluyeron prospectivamente durante 24 meses todos los pacientes con sospecha clínica intermedia de coledocolitiasis y CRM negativa. Se compar�� la sensibilidad, especificidad, precisión diagnóstica, valor predictivo positivo y negativo de CRM y USE, así como su impacto en el tratamiento. Resultados: se evaluaron 76 pacientes (litiasis en el 30%. La sensibilidad y precisión diagnóstica de USE (100%, 92% eran significativamente

  3. CITYMARKETING EN CIUDADES INTERMEDIAS: CASO PALMIRA

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    Miguel Ezequiel Badillo Mendoza

    2010-01-01

    Full Text Available En este artículo se presentan los resultados de la investigación Citymarketing en ciudades intermedias: caso Palmira, específicamente en su etapa de diseño. Este proyecto surgió de la necesidad de generar acciones académicas y metodológicas para desarrollar un mecanismo que permitiera el diseño de un instrumento de gestión y promoción de la ciudad. La población de estudio se integró con habitantes de la ciudad, visitantes y dirigentes de diversos sectores del departamento del Valle del Cauca. La metodología empleada se basó en un estudio documental y el uso de instrumentos cualitativos, como cartografías urbanas, estudio de imaginarios y mediciones cuantitativas de comunicación publicitaria. Para su realización se estructuró en dos fases: La primera donde se estableció un eje conceptual para la implementación de un Plan de citymarketing en Palmira y una fase 2: donde se desarrolló la sensibilización, la caracterización de la ciudad, los lineamientos estratégicos, los parámetros para la construcción de la marca y el diseño del Plan de Comunicaciones.

  4. Content-based intermedia synchronization

    Science.gov (United States)

    Oh, Dong-Young; Sampath-Kumar, Srihari; Rangan, P. Venkat

    1995-03-01

    Inter-media synchronization methods developed until now have been based on syntactic timestamping of video frames and audio samples. These methods are not fully appropriate for the synchronization of multimedia objects which may have to be accessed individually by their contents, e.g. content-base data retrieval. We propose a content-based multimedia synchronization scheme in which a media stream is viewed as hierarchial composition of smaller objects which are logically structured based on the contents, and the synchronization is achieved by deriving temporal relations among logical units of media object. content-based synchronization offers several advantages such as, elimination of the need for time stamping, freedom from limitations of jitter, synchronization of independently captured media objects in video editing, and compensation for inherent asynchronies in capture times of video and audio.

  5. Fluxacademy: From Intermedia to Interactive Education.

    Science.gov (United States)

    Saper, Craig

    1992-01-01

    Advocates a Fluxus-based experimental pedagogy which is suited for scholarship confronted with film and electronic media. Notes that the theory explored in Fluxacademy focuses specifically on the use of intermedia for interactive education. (RS)

  6. Influencia de la actividad física, en el desarrollo motor de los estudiantes de básica intermedia, con discapacidad auditiva, de la Unidad Educativa Especial Claudio Neira, (octubre - marzo 2015)

    OpenAIRE

    Naranjo Naranjo, Edwin Geovanny; Vera Bustamante, Braulio Ricardo

    2015-01-01

    Este proyecto demuestra que la actividad física, si influye en el desarrollo motor de los estudiantes con discapacidad sensorial auditiva de la UEE Claudio Neira; fundamentados en cuatro capítulos se investigaron, seleccionaron y aplicaron varios test y estrategias para mejorar las capacidades físicas, coordinativas y habilidades motrices básicas de los estudiantes. This project shows how the physical activity is a good stimulation for the student development whit special sensorial hearing...

  7. Aislamiento de tres cepas de Leishmanía a partir de Lutzomyia Trapidoi en Colombia

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    Alberto Morales

    1981-12-01

    Full Text Available En un foco de Leishmaniasis Cutánea en un bosque en la vereda Flor Azul, municipio de Mariquita, Departamento del Tolima, Colombia, se recolectaron, usando trampas de luz (C D C, trampas Shannon. cebo humano, cebo animal (caballo y captura en huecos de árboles de febrero de 1979 a mayo de 1981, un total de 4.081 y 7.912 de Lutzomyia (Diptera, Psychodidae, las cuales pertenecían a 19 especies. De las 7.912 hembras recolectadas se hizo disección del tubo digestivo a 3.337 multíparas en busca de flagelados, los cuales se encontraron en: una Lutzomyia shannoni, una Lutzomyia gomeziy cuarenta y dos Lutzomyia trapidoi. De estos 44 intestinos con flagelados se inocularon 32 hamsters, con el siguiente resultado: 2 hamsters murieron por causas desconocidas (el de L. shannoni y L. gomezl;  de 30 hamsters inoculados con flagelados hallados en intestino de L. trapidoi tres fueron positivos para Leishmanai, 12 permanecen en observación y son negativos hasta la fecha y 15 murieron por causas desconocidas. Las tres cepas de Leishmania aisladas de L. trapidoise mantienen en hamsters y en cultivos en medio de las 4 N. Del total de 2.869 hembras de Lutzomyia trapidoi disectadas. 2.356 eran multíparas; de éstas se encontraron 42 con flagelados en el intestino. Por la localización de los flagelados en el intestino anterior de L. trapidoi en una de las cepas aisladas, por el tan corto período de incubación de las cepas inoculadas en el hamster y por la facilidad con la cual se cultivan, es posible pensar que estas cepas pertenezcan al complejo Leishmania mexicana. Esta es la primera vez que en Colombia se aislan cepas de Leishmania del intestino del insecto vector.

  8. Capacidad entomocida de cepas nativas de Beauveria sp. sobre Schistocerca piceifrons peruviana (Lynch Arribalzaga, 1903

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    Nicolaza Pariona

    2013-04-01

    Full Text Available Cuatro cepas de Beauveria sp. (ABvPr11, ABvPr8, ABvPr3 y ABvSr4, fueron aisladas y caracterizadas de ejemplares de la langosta Schistocerca piceifrons peruviana procedentes de la Provincia de Huamanga del Departamento de Ayacucho. La capacidad entomocida de las 4 cepas fue evaluada en la langosta Schistocerca piceifrons peruviana. Los resultados muestran que todas las cepas, en soluciones de 108 conidias/mL, producen un 100% de mortalidad. El tiempo que se necesitó para eliminar el 100% de las langostas, para las cepas ABvPr11, ABvPr8, ABvSr4 y ABvPr3 fue de 12, 14 y 16 días respectivamente. Al décimo día se observó diferencias significativas respecto a la mortalidad, entre la cepa ABvPr11(90% y la cepas ABvSr4(74%, ABvPr8(72% y ABvPr3(64.6%. Posteriormente se determinó el tiempo de letalidad al 50% (TL50 de la población de langostas, con un intervalo de 8,38 a 9,16 días y el tiempo de letalidad al 80% (TL80 fue de 9,6 a 11,5 días. La cepa ABvPr11 presentó la mayor actividad entomocida para el control de Schistocerca piceifrons peruviana L.A.

  9. Efecto del Fosfito de Potasio en Combinación con el Fungicida Metalaxyl + Mancozeb en el Control de Mildeo Velloso (Peronospora destructor Berk en Cebolla de Bulbo (Allium cepa L. Effect of the Potasium Phosphite in Combination with the Fungicide Metalaxyl plus Mancozeb on the Control of Downy Mildew (Peronospora destructor Berk in Onion Bulb (Allium cepa L.

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    Jorge Velandia Monsalve

    2012-06-01

    Full Text Available Resumen. El hongo Peronospora destructor causa considerables pérdidas en la producción de cebolla de bulbo en el mundo. Fosfito de potasio en combinación con el fungicida Metalaxyl + Mancozeb, fue evaluado por su eficiencia en el control de este patógeno en el híbrido de cebolla de bulbo Yellow Granex. Los tratamientos consistieron en una aplicación de fosfito (5 mL L-1 alternada con una de fungicida (2 g L-1; dos y tres aplicaciones consecutivas de fosfito alternadas con una de fungicida; una aplicación de fungicida alternada con una de fosfito; dos y tres aplicaciones consecutivas de fungicida alternadas con una de fosfito; aplicaciones de sólo fosfito y sólo fungicida y un testigo. Se utilizó el diseño experimental de bloques completos al azar con tres repeticiones. Se evaluó la incidencia y severidad de la enfermedad, el número, tamaño y peso de bulbos de primera, segunda, tercera y peso total de bulbos. Los resultados mostraron que las aplicaciones de fosfito de potasio durante dos semanas consecutivas alternadas en la tercera semana con una de fungicida y las aplicaciones semanales de sólo fosfito, tuvieron efectos altamente significativos en el control de la incidencia y severidad de P. destructor y diferencias significativas en el peso de los bulbos de primera (28,1 y 27,9 t ha-1 y peso total de bulbos (55,5 y 52,1 t ha-1, representando en relación al testigo, un incremento de 200 y 196% en el peso de los bulbos de primera y de 55 y 45,7% en el peso total de bulbos, respectivamente. Se concluye que con el fosfito de potasio se reduce la aplicación de fungicidas y ello es una alternativa viable para el manejo ecológico de P. destructor en la producción de cebolla de bulbo.Abstract. Peronospora destructor fungi causes considerable loses in the production of onion bulb in the world. Potassium phosphite in combination with the fungicide Metalaxyl + Mancozeb was evaluated for its efficiency in the control of this pathogen

  10. Virulencia de cepas de Listeria monocytogenes procedentes de cabras y sus derivados

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    Guicela Ramírez Bernal

    2014-01-01

    Full Text Available Se evaluó la virulencia de cepas de Listeria monocytogenes todas de serotipo 4b procedentes de cabras y sus derivados. Se observaron niveles de virulencia variables cuando se comparó la virulencia relativa (porcentaje de letalidad en ratones BALB/c inoculados vía intravenosa o intragástrica y su capacidad para infectar macrófagos J774A.1, y células epiteliales Caco-2. Dos cepas obtenidas de alimento de cabras produjeron 100 % de letalidad por ambas vías de inoculación y no mostraron diferencia significativa con la cepa testigo (P>0.05 respecto al porcentaje de invasión y a los parámetros de la cinética de crecimiento cuadrática observada en ambas líneas celulares. Si bien todas las cepas lograron invadir las células Caco-2, solamente algunas consiguieron invadir el bazo después de la inoculación por vía intragástrica. Las dos cepas provenientes de alimento de cabras fueron las más virulentas, representando un riesgo para la salud humana y animal, ya que pueden ser diseminadas en el hato y de este a otras explotaciones o a las instalaciones donde se elaboran alimentos.

  11. Compatibilidad entre nueve cepas de Biomphalaria glabrata de áreas endémicas y no endémicas y una cepa de Schistosoma mansoni venezolanas

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    Pino Luz A.

    1999-01-01

    Full Text Available Se infectaron experimentalmente 9 lotes de 32 caracoles B. glabrata (de 5 a 7mm de diámetro con miracidios de la cepa C5 de Schistosoma mansoni a razón de 5 miracidios por caracol, pertenecientes a las siguientes cepas: En el área endémica de transmisión de Esquistosomiasis mansoni a Sector Puerta Negra, Lago Valencia, b Cagua c Ingenio Bolívar (Estado Aragua d Mariara e Caserío El 25 f Güigüe (Estado Carabobo. Fuera del área endémica de transmisión g Anzoátegui (Estado Lara, h Chabasquén (Estado Portuguesa, i Sector La Elvira, Caripe (Estado Monagas. El período prepatente intramolusco, osciló entre 23 y 25 días, para las 9 cepas evaluadas. La duración total de la infección fue muy variable desde 20 días para la cepa Chabasquén, hasta 93 días para la de Güigüe.La producción total promedio de cercarias al tercer día de iniciada la emisión varió desde X = 74,4 para la cepa de Mariara, hasta X = 591,7 para la cepa de Chabasquén. Se evidenció la existencia de diferencias estadísticamente significativas (H = 97,4, P < 0,05 en la producción total de cercarias al tercer día de iniciada la emisión, detectándose diferencias estadísticamente significativas para casi todas las 36 combinaciones, excepto para las cuatro siguientes: Mariara/Ingenio Bolívar, Cagua/Caserío El 25, Lago de Valencia/Güigüe y Güigüe/Caripe.En lo que respecta al porcentaje de caracoles que presentaron cura espontánea, los valores mas elevados se obtuvieron en las cepas del Lago de Valencia (88,8%, Cagua (85,2%, Chabasquén (82,6%, Caripe (82,6% y Anzoátegui (80%. Mientras que el porcentaje mas bajo se obtuvo para la cepa de Güigüe (21,4%.

  12. EFECTIVIDAD DE CEPAS DE HMA EN EL CULTIVO DE LA YUCA (Manihot esculenta Crantz) EN DOS TIPOS DE SUELOS

    OpenAIRE

    José P. João; Alberto Espinosa Cuellar; Luís Ruiz Martínez; Jaime Simó González; Ramón Rivera Espinosa

    2016-01-01

    Con el objetivo de evaluar la efectividad de la inoculación de cepas de hongos micorrízicos arbusculares (HMA) en clones comerciales de yuca (Manihot esculenta Crantz) y su dependencia con el tipo de suelo, se comparó la efectividad de tres cepas de HMA, Glomus cubense, Rizophagus intraradices y Funneliformis mosseae, tanto en suelo Pardo Mullido Carbonatado como en Ferralítico Rojo Lixiviado. En el primer suelo, se utilizaron seis clones comerciales en un diseño de bloq...

  13. India-Korea CEPA: Potentials and Realities

    OpenAIRE

    Ahmed, Shahid

    2010-01-01

    The present study investigates the potential economic impacts of India- Korea CEPA using trade indices, partial equilibrium and computable general equilibrium. One hypothetical scenario is examined in SMART model and two hypothetical tariff liberalization scenarios are examined in GTAP model focusing on short run and long run. Using the partial equilibrium WITS-SMART model, we tried to assess the impact of liberalization under the CEPA, assuming full liberalization of imports from the India i...

  14. Variabilidad genética en cepas de Sporothrix schenckii aisladas en Abancay, Perú

    Directory of Open Access Journals (Sweden)

    Susan Holechek

    2004-04-01

    Full Text Available Objetivo: Identificar los genotipos de S. schenckii que circulan en 2 distritos de la provincia de Abancay, Perú. Material y Métodos: Se evaluaron 17 cepas procedentes de pacientes con lesiones linfocutáneas y lesión cutánea fija mediante la técnica del ADN Polimorfo Amplificado Aleatorio - Reacción en Cadena de la Polimerasa (RAPD - PCR con el cebador GTG 5 (GTG GTG GTG GTG GTG. Resultados: Identificamos 6 genotipos, siendo el genotipo I el predominante en las áreas de estudio. No se logró asociar los genotipos obtenidos con caracteres clínicos y geográficos. Conclusiones: Nuestros resultados evidencian que existe biodiversidad genética entre las cepas de S. schenckii que circulan en ambas zonas.

  15. Plasmidotipia y evaluación in vitro de la virulencia de cepas de Salmonella enteritidis de origen aviar

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    Rubén Darío Toro

    2000-02-01

    Full Text Available Con el fin de caracterizar a nivel biológico y molecular cepas de Salmonella entérica, subespecie entérica serotipo Enteritidis (Salmonella enteritidis se obtuvieron y confirmaron bioquímica y serológicamente treinta cepas aisladas de aves clínicamente sanas. Las cepas fueron evaluadas mediante plasmidotipia y determinación de virulencia in vitro evaluando susceptibilidad antimicrobiana, actividad hemaglutinante manosa resistente y manosa sensible como indicativo de la presencia de adhesinas fimbriales y capacidad de sobrevivencia en el interior de macrófagos. La extracción de plásmidos se realizó por la técnica de lisis alcalina. Se obtuvieron perfiles de restricción con las enzimas PstI y SmaI. La susceptibilidad antimicrobiana se determinó por la prueba de difusión de disco en agar. La actividad hemaglutinante en presencia y en ausencia de manosa fue evaluada frente a glóbulos rojos de ave, bovino, equino, humano (A+ y ovino. La capacidad de sobrevivencia en el interior de macrófagos se determinó valorando la viabilidad de las cepas 24 horas después de la ingestión por macrófagos de línea de origen murino. Veintinueve cepas (96.6% mostraron un plasmido cuyo tamaño corresponde al plasmido de virulencia asociado a serotipo. Veintisiete cepas (90% mostraron dos o más plasmidos. Perfiles de restricción realizados con las enzimas Pst I y Sma I permitieron establecer una alta asociación entre las cepas. Se determinó resistencia a uno o más antimicrobianos en catorce cepas de S. enteritidis (46.6%, se observó resistencia a oxitetraciclina, sulfatrimetroprim, estreptomicina y cloranfenicol. Ninguna cepa mostró resistencia a gentamicina y kanamicina. Se detectó actividad hemaglutinante manosa sensible como indicativo de adhesinas fimbriales tipo I en once cepas (36.6% de las treinta evaluadas. La totalidad de las cepas sobrevivieron en el interior de macrófagos de línea J774A.1 y fueron viables 24 horas después de la

  16. Nuevos sistemas de frecuencia intermedia para el IAR

    Science.gov (United States)

    Olalde, J. C.; Perilli, D.; Larrarte, J. J.

    Se presenta el diagrama en bloques de los nuevos sistemas de Frecuencia Intermedia para los dos radiómetros instalados en el IAR. Entre las características más importantes del sistema podemos mencionar la posibilidad de conectar cualquiera de las dos antenas a los ``backend" disponibles: analizador espectral de alta resolución (META II) de 0,05 Hz, autocorrelador de 1008 canales y contínuo. Se incorporan al sistema nuevos sintetizadores de frecuencia implementados con PLL y la moderna técnica de síntesis digital directa. Por último, el conjunto del sistema es susceptible de ser configurado por las computadoras de adquisición de datos, supervisadas por otra, que entrega el estado de funcionamiento actual y evita la selección de configuraciones incorrectas por parte del usuario.

  17. Transfusion regimens in thalassemia intermedia

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    Z. Karakas

    2011-12-01

    Full Text Available Thalassemia intermedia (TI is a heterogeneous disease, in terms of both clinical manifestations and underlying molecular defects. Some TI patients are asymptomatic until adult life, whereas others are symptomatic from early childhood. In contrast with patients with Thalassemia major (TM, the severity of anemia is less and the patients do not require transfusions during at least the first few years of life. Many patients with TI, especially older ones, have been exposed to the multiple long-term effects of chronic anemia and tissue hypoxia and their compensatory reactions, including enhanced erythropoiesis and increased iron absorption. Bone marrow expansion and extramedullary hematopoiesis lead to bone deformities and liver and spleen enlargement. Therapeutic strategies in TI are not clear and different criteria are used to decide the initiation of transfusion and chelation therapy, modulation of fetal hemoglobin production, and hematopoietic stem cell transplantation on an individual basis. The clinical picture of well-treated TM patients with regular transfusionchelation therapy is better from TI patients who have not received adequate transfusion therapy. There is a significant role of early blood transfusion to prevent and treat complications commonly associated with TI, such as extramedullary erythropoiesis and bone deformities, autoimmune hemolytic anemia, leg ulcers, gallstones, pseudoxantoma elasticum, hyperuricosuria, gout and pulmonary hypertension, which are rarely seen in thalassemia major. Nowadays, indications of transfusion in patients with TI are chronic anemia (Hb < 7 g/dL, bone deformities, growth failure, extramedullary erythropoiesis, heart failure, pregnancy and preparation for surgical procedures. Conclusion: Adequate (regular or tailored transfusion therapy is an important treatment modality for increasing the quality of life in patients with thalassemia intermedia during childhood. 就临床表象和潜在的分子缺

  18. Efectos del gas ozono sobre cepas de Fusarium verticillioides y F. proliferatum productoras de fumonisinas

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    Laura Frisón

    2014-07-01

    Full Text Available Las fumonisinas son producidas por especies de Fusarium, esencialmente por Fusarium verticillioides y F. proliferatum que se encuentran como contaminantes naturales en maíz y subproductos de maíz. Su consumo, se asocia con ciertas enfermedades en animales y humanos. La industria alimentaria apunta sus investigaciones al desarrollo de nuevas tecnologías y la aplicación de gas ozono, dado su elevado poder germicida y su descomposición espontánea a oxígeno, se ha convertido en un agente potencial para garantizar la seguridad microbiológica y la calidad de los alimentos. Debido a la importancia del consumo de maíz en Argentina y a la contaminación de este grano por cepas productoras de fumonisinas, se estudió la posibilidad de detoxificar maíz contaminado con esta toxina con gas ozono. Se aislaron cepas de Fusarium verticillioides y F. proliferatum de granos de maíz y de silaje. Se estudió la capacidad de producción de toxina de dichas cepas. La cuantificación de esta toxina se realizó mediante ensayo por inmunoabsorción ligado a enzimas (ELISA con el kit para fumonisinas RIDASCREEN®FAST Fumonisin siguiendo las instrucciones del fabricante. Se utilizó gas ozono a concentraciones de: 4500, 7500 y 25000 ppmv, por tiempos de exposición de 10 y 20 minutos. Todas las cepas de Fusarium estudiadas fueron buenas productoras de fumonisinas. A las concentraciones y tiempos evaluados, no se observó la eliminación o disminución de la concentración de toxina. Prevenir la contaminación con estos mohos es la mejor solución para el problema de las micotoxinas, debido a que una vez producida la toxina es difícil de erradicar.

  19. Efectos del gas ozono sobre cepas de Fusarium verticillioides y F. proliferatum productoras de fumonisinas

    Directory of Open Access Journals (Sweden)

    Laura Frisón

    2014-06-01

    Full Text Available Las fumonisinas son producidas por especies de Fusarium, esencialmente por Fusarium verticillioides y F. proliferatum que se encuentran como contaminantes naturales en maíz y subproductos de maíz. Su consumo, se asocia con ciertas enfermedades en animales y humanos. La industria alimentaria apunta sus investigaciones al desarrollo de nuevas tecnologías y la aplicación de gas ozono, dado su elevado poder germicida y su descomposición espontánea a oxígeno, se ha convertido en un agente potencial para garantizar la seguridad microbiológica y la calidad de los alimentos. Debido a la importancia del consumo de maíz en Argentina y a la contaminación de este grano por cepas productoras de fumonisinas, se estudió la posibilidad de detoxificar maíz contaminado con esta toxina con gas ozono. Se aislaron cepas de Fusarium verticillioides y F. proliferatum de granos de maíz y de silaje. Se estudió la capacidad de producción de toxina de dichas cepas. La cuantificación de esta toxina se realizó mediante ensayo por inmunoabsorción ligado a enzimas (ELISA con el kit para fumonisinas RIDASCREEN®FAST Fumonisin siguiendo las instrucciones del fabricante. Se utilizó gas ozono a concentraciones de: 4500, 7500 y 25000 ppmv, por tiempos de exposición de 10 y 20 minutos. Todas las cepas de Fusariumestudiadas fueron buenas productoras de fumonisinas. A las concentraciones y tiempos evaluados, no se observó la eliminación o disminución de la concentración de toxina. Prevenir la contaminación con estos mohos es la mejor solución para el problema de las micotoxinas, debido a que una vez producida la toxina es difícil de erradicar.

  20. Compatibilidad entre nueve cepas de Biomphalaria glabrata de áreas endémicas y no endémicas y una cepa de Schistosoma mansoni venezolanas

    OpenAIRE

    Pino,Luz A.; Matinella,Liboria; Morales C.,Gustavo

    1999-01-01

    Se infectaron experimentalmente 9 lotes de 32 caracoles B. glabrata (de 5 a 7mm de diámetro) con miracidios de la cepa C5 de Schistosoma mansoni a razón de 5 miracidios por caracol, pertenecientes a las siguientes cepas: En el área endémica de transmisión de Esquistosomiasis mansoni a) Sector Puerta Negra, Lago Valencia, b) Cagua c) Ingenio Bolívar (Estado Aragua) d) Mariara e) Caserío El 25 f) Güigüe (Estado Carabobo). Fuera del área endémica de transmisión g) Anzoátegui (Estado Lara), h) Ch...

  1. β-Thalassemia Intermedia: A Clinical Perspective

    Science.gov (United States)

    Musallam, Khaled M.; Taher, Ali T.; Rachmilewitz, Eliezer A.

    2012-01-01

    Our understanding of the molecular and pathophysiological mechanisms underlying the disease process in patients with β-thalassemia intermedia has substantially increased over the past decade. Earlier studies observed that patients with β-thalassemia intermedia experience a clinical-complications profile that is different from that in patients with β-thalassemia major. In this article, a variety of clinical morbidities are explored, and their associations with the underlying disease pathophysiology and risk factors are examined. These involve several organs and organ systems including the vasculature, heart, liver, endocrine glands, bone, and the extramedullary hematopoietic system. The effects of some therapeutic interventions on the development of clinical complications are also discussed. PMID:22762026

  2. Susceptibilidad antimicrobiana in vitro de cepas de Neisseria gonorrhoeae procesadas en el instituto nacional de salud, Lima, Perú. 1998-1999

    Directory of Open Access Journals (Sweden)

    José Portilla C

    2003-10-01

    Full Text Available Objetivo: Determinar la frecuencia de susceptibilidad y resistencia in vitro de cepas de Neisseria gonorrhoeae, aisladas en el Laboratorio de Bacterias de Transmisión Sexual del Instituto Nacional de Salud, Lima, Perú, frente a los antimicrobianos de uso actual en el tratamiento de la infección por el gonococo; asimismo, conocer la frecuencia con que se presentan las cepas de N. gonorrhoeae productoras de beta-lactamasas. Material y Métodos: Estudio observacional descriptivo. En el Laboratorio de Bacterias de Transmisión Sexual del Instituto Nacional de Salud, Lima, Perú, se recibieron 130 cepas de N. gonorrhoeae (49 en 1998 y 81 en 1999, procedentes de diferentes localidades del país para confirmación bacteriológica. El método de estudio de susceptibilidad antimicrobiana usado fue el de Concentración inhibitoria mínima (CIM frente a la penicilina, espectinomicina, ceftriaxona, cefotaxima, ciprofloxacina y azitromicina. Resultados: La frecuencia de la resistencia de las cepas de N. gonorrhoeae in vitro a la penicilina fue en 15/49 cepas en el año 1998 y 15/81 cepas en el año 1999. Asimismo, la resistencia a la tetraciclina fue en 31/49 cepas en el año 1998 y 51/81 cepas en 1999. El 100% de las cepas estudiadas fueron sensibles a la espectinomicina, ceftriaxona, cefotaxima y ciprofloxacina. En el país, se reporta en 9/81 cepas de N. gonorrhoeae de susceptibilidad disminuida a la azitromicina. Entre los años 1998 y 1999, se reportaron 21/52 y 6/85, respectivamente de cepas de N. gonorrhoeae productoras de beta-lactamasas. Conclusiones: Se encontró resistencia in vitro de cepas de N. gonorrhoeae frente a la penicilina y tetraciclina. Los antibióticos que se deben usar para el tratamiento de la infección son: espectinomicina, ceftriaxona, cefotaxima y ciprofloxacina. Por primer vez se reporta en el país, cepas de susceptibilidad disminuida a la azitromicina.

  3. Selección de cepas productoras de enzimas ligninolíticas nativas del Valle del Yaqui

    Directory of Open Access Journals (Sweden)

    S.R. Maldonado-Bustamante

    2017-01-01

    Full Text Available Introducc ión: Las fibras lignocelulósicas están compuestas por tres polímeros: celulosa, hemicelulosa y lignina. El biopulpeo es un pretratamiento, previo al proceso de pulpeo físico químico, cuyo fin es lograr la deslignificación parcial del complejo lignocelulós ico por la acción de hongos, debido a que los hongos tienen un complejo enzimático formado por: ligno peroxidasas (LiP, lacasas (Lac, manganeso peroxidasas (MnP, y xilanasas (Xil. Las cepas nativas tienen la habilidad de deslignificar el material ligno celulósico como paja de trigo o madera, entre otros. Método: Se trabajó con diez cepas de la colección COLMENA del Laboratorio de Biotecnología del Recurso Microbiano del Instituto Tecnológico de Sonora. La selección de cepas se realizó escogiendo las que presentaron mayor índice de potencia (I.P., obtenido a través de la comparación del halo de actividad enzimática contra el halo de crecimiento de la cepa, a los 14 días de incubación utilizando el software Image J 1.44. Resultados: De las diez cepas eva luadas , la mejor para la producción de LiP fue TSM35 con un I.P. de 3.83±0.29, para Lac y Xil ; la cepa TSO46 obtuvo valores de con un I.P. de 20.37±2.70 y 1.38±0.00 respectivamente . Con este estudio se determinó el potencial de incorporar cepas nativas pa ra el pretratamiento de biopulpeo previo a un un proceso de deslignificación físico químico. Discusiones o Conclusiones: Se logró determinar que tres cepas tienen actividad ligninolítica en medio sólido por excreción de LiP, Lac y Xil , por lo que tiene n potencial para incorporarlos como pretratamiento en un proceso de deslignificación.

  4. Del Distrito Industrial al Desarrollo Territorial: estrategias de innovación en ciudades intermedias

    Directory of Open Access Journals (Sweden)

    Ricardo Méndez

    2006-01-01

    Full Text Available Los contrastes territoriales se ha incrementado desde el inicio de los procesos de globalización e integración, pero, al mismo tiempo, las ciudades están mostrando diferente capacidad de respuesta ante esas transformaciones. Dentro de la Unión Europea, el policentrismo y el papel de las ciudades intermedias han cobrado creciente importancia en la actual agenda política, especialmente en el caso de las regiones periféricas con problemas estructurales que dificultan su desarrollo. El artículo hace una revisión crítica del paradigma de los distritos industriales y los ambientes innovadores para centrarse en su posible utilidad en la búsqueda de las claves para la construcción de ciudades intermedias innovadoras. Se concede especial importancia a los actores locales, las redes institucionales, la organización de sistemas productivos locales y la gobernabilidad territorial. Por último, se plantean algunas propuestas para apoyar la consolidación de procesos innovativos en ciudades intermedias.

  5. Detection and genetic characterization of β-lactamases in Prevotella intermedia and Prevotella nigrescens isolated from oral cavity infections and peritonsillar abscesses.

    Science.gov (United States)

    Fernández-Canigia, Liliana; Cejas, Daniela; Gutkind, Gabriel; Radice, Marcela

    2015-06-01

    A prospective analysis on β-lactam resistance mechanisms and β-lactamase prevalence was conducted on Prevotella intermedia and Prevotella nigrescens recovered from patients with chronic periodontitis and peritonsillar abscesses. Both phenotypic and genotypic methods were performed to characterize the β-lactamases, their coding genes and their genetic contexts. Overall, β-lactamase production was observed in 64% (16/25) P. intermedia and 23.8% (5/21) P. nigrescens (p intermedia (8/16) than in P. nigrescens (2/16) recovered from chronic periodontitis, almost all isolates from peritonsillar abscesses were producers (8/9 and 3/3, respectively). cfxA, but not cepA and cblA, was detected in those isolates, which were previously categorized as β-lactamase producers. CfxA producing isolates displayed higher β-lactam MICs than non-producers in both species. The most frequent allele was cfxA2, followed by cfxA3 and a new allelic variant named cfxA6. The analysis of the downstream flanking region in the three cfxA variants revealed the association with mobA of Tn4555, suggesting their localization in a mobilizable element. β-lactam resistance and cfxA carriage prevalence seems to be not only related to the bacterial species but also to the infection site. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Echocardiographic evaluation of thalassemia intermedia patients in Duhok, Iraq.

    Science.gov (United States)

    Mohammad, Ameen Mosa

    2014-12-11

    Cardiac complications are among the most serious problems of thalassemia intermedia patients. The current study was initiated to address the latter issue through the study of the echocardiographic findings and correlate it with clinical characteristics of thalassemia intermedia patients in Duhok, Kurdistan region, Iraq. An echocardiographic assessment of 61 beta-thalassemia intermedia cases was performed. It included 30 males and 31 females, with a mean age 19.6 ± 7.5 years. The standard echostudy of two-dimension and M-mode measurements of cardiac chambers were done. The continuous doppler regurgitant jet of tricuspid and pulmonary valves were recorded. Left ventricle diastolic function was assessed by pulsed doppler of mitral valve inflow. To correlate the clinical with echocardiographic findings, patients were divided, according to tricuspid regurgitant velocity, into three groups (intermedia patients. Therapeutic trails targeting these complications are indicated, and echocardiographic assessment is necessary to be offered early for thalassemia intermedia.

  7. Ruptured Massa Intermedia Secondary to Hydrocephalus.

    Science.gov (United States)

    El Damaty, Ahmed; Langner, Soenke; Schroeder, Henry W S

    2017-01-01

    We report a case of ruptured massa intermedia (MI) as a sequela of hydrocephalus. A single case report is presented describing the sequelae of tumor bed hematoma after a posterior fossa hemangioblastoma resection in which the patient bled 3 days after surgery, resulting in secondary hydrocephalus and subsequently dilatation of the third ventricle, which resulted in rupture of the MI. The patient was managed on emergency basis with an external ventricular drain then endoscopically with a third ventriculostomy and clot extraction. Absent MI is not uncommon in hydrocephalic patients, and it is assumed to be the result of rupture from acute dilatation of the third ventricle. Our case report proves this assumption and documents the presence and absence of the MI before and after developing hydrocephalus. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. β-Thalassemia intermedia: a comprehensive overview and novel approaches.

    Science.gov (United States)

    Asadov, Chingiz; Alimirzoeva, Zohra; Mammadova, Tahira; Aliyeva, Gunay; Gafarova, Shahla; Mammadov, Jeyhun

    2018-01-29

    β-Thalassemia intermedia is a clinical condition of intermediate gravity between β-thalassemia minor, the asymptomatic carrier, and β-thalassemia major, the transfusion-dependent severe anemia. It is characterized by a significant clinical polymorphism, which is attributable to its genetic heterogeneity. Ineffective erythropoiesis, chronic anemia, and iron overload contribute to the clinical complications of thalassemia intermedia through stepwise pathophysiological mechanisms. These complications, including splenomegaly, extramedullary erythropoiesis, iron accumulation, leg ulcers, thrombophilia, and bone abnormalities can be managed via fetal hemoglobin induction, occasional transfusions, chelation, and in some cases, stem cell transplantation. Given its clinical diversity, thalassemia intermedia patients require tailored approaches to therapy. Here we present an overview and novel approaches to the genetic basis, pathophysiological mechanisms, clinical complications, and optimal management of thalassemia intermedia.

  9. Hepatoprotective effects of Allium cepa (onion) extracts against ...

    African Journals Online (AJOL)

    SAM

    2014-06-25

    Jun 25, 2014 ... against paracetamol-induced liver damage in rats. Ozougwu, J. C.1* and ... Key words: Allium cepa, paracetamol, hepatoprotective effects, alanine aminotransferase, aspartate ...... function tests to different stages of chronic liver diseases in HCV .... activity of alcoholic and aqueous extacts of Allium cepa linn.

  10. Evaluación de la fijación de nitrógeno por cepas de Rhizobium que nodulan Frijol (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    María Inés Ballesteros

    2010-07-01

    Full Text Available La capacidad de fijar nitrógeno de ocho cepas de Rhizohium fue evaluada incdiante dos ensayos de invernadero, en solución nutritiva, con cinco variedades de fríjol: ICA CITARA, ICA PIJAO, DIACOL CATIO, ANDINO y PVA 916. Se determinó en floración el peso seco de la parle aérea, peso seco de nodulos y nitrógeno total en la planta por el método de microkjeldahi. Las cepas de mejor comportamiento en cuanto a nitrógeno fijado fueron en su orden : CIAT 144, CIAT 899, y CIAT 2; aunque en general la respuesta de las cepas fue diferente con la variedad de Iríjol se logró un porcentaje promedio de nitrógeno fijado del orden del 28% comparado con los controles que recibieron nitrógeno combinado (Ensayo 2. Las cepas CIAT 71 15 y CIAT 632 presentaron la menor efectividad con las variedades ensayadas. Se encontró correlación altamente significativa entre el rendimiento en peso seco y el nitrógeno fijado para todas las variedades de fríjol.

  11. Antibacterial and antibiofilm effects of iron chelators against Prevotella intermedia.

    Science.gov (United States)

    Moon, Ji-Hoi; Kim, Cheul; Lee, Hee-Su; Kim, Sung-Woon; Lee, Jin-Yong

    2013-09-01

    Prevotella intermedia, a major periodontopathogen, has been shown to be resistant to many antibiotics. In the present study, we examined the effect of the FDA-approved iron chelators deferoxamine (DFO) and deferasirox (DFRA) against planktonic and biofilm cells of P. intermedia in order to evaluate the possibility of using these iron chelators as alternative control agents against P. intermedia. DFRA showed strong antimicrobial activity (MIC and MBC values of 0.16 mg ml(-1)) against planktonic P. intermedia. At subMICs, DFRA partially inhibited the bacterial growth and considerably prolonged the bacterial doubling time. DFO was unable to completely inhibit the bacterial growth in the concentration range tested and was not bactericidal. Crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that DFRA significantly decreased the biofilm-forming activity as well as the biofilm formation, while DFO was less effective. DFRA was chosen for further study. In the ATP-bioluminescent assay, which reflects viable cell counts, subMICs of DFRA significantly decreased the bioactivity of biofilms in a concentration-dependent manner. Under the scanning electron microscope, P. intermedia cells in DFRA-treated biofilm were significantly elongated compared to those in untreated biofilm. Further experiments are necessary to show that iron chelators may be used as a therapeutic agent for periodontal disease.

  12. Prevotella intermedia induces prostaglandin E2 via multiple signaling pathways.

    Science.gov (United States)

    Guan, S-M; Fu, S-M; He, J-J; Zhang, M

    2011-01-01

    Prostaglandin E(2) (PGE(2)) plays important roles in the bone resorption of inflammatory diseases such as rheumatoid arthritis and periodontitis via specific prostaglandin receptors (i.e., EP1-EP4). In this study, the authors examined whether Prevotella intermedia regulates PGE(2) production and EP expression in human periodontal ligament fibroblasts (hPDLs); they also explored the potential signaling pathways involved in PGE(2) production. P. intermedia induced PGE(2) production and cyclooxygenase-2 (COX-2) expression in a dose- and time-dependent manner. Indomethacin and NS-398 completely abrogated the P. intermedia-induced PGE(2) production without modulating COX-2 expression. Specific inhibitors of extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38, phosphatidylinositol 3-kinase, and protein kinase C--but not c-AMP and protein kinase A--significantly attenuated the P. intermedia-induced COX-2 and PGE(2) expression. P. intermedia reduced EP1 expression in a concentration- and time-dependent manner. The results indicate that the COX-2-dependent induction of PGE(2) by P. intermedia in hPDLs is mediated by multiple signaling pathways.

  13. Invasion of Human Oral Epithelial Cells by Prevotella intermedia

    Science.gov (United States)

    Dorn, Brian R.; Leung, K.-P.; Progulske-Fox, Ann

    1998-01-01

    Invasion of oral epithelial cells by pathogenic oral bacteria may represent an important virulence factor in the progression of periodontal disease. Here we report that a clinical isolate of Prevotella intermedia, strain 17, was found to invade a human oral epithelial cell line (KB), whereas P. intermedia 27, another clinical isolate, and P. intermedia 25611, the type strain, were not found to invade the cell line. Invasion was quantified by the recovery of viable bacteria following a standard antibiotic protection assay and observed by electron microscopy. Cytochalasin D, cycloheximide, monodansylcadaverine, and low temperature (4°C) inhibited the internalization of P. intermedia 17. Antibodies raised against P. intermedia type C fimbriae and against whole cells inhibited invasion, but the anti-type-C-fimbria antibody inhibited invasion to a greater extent than the anti-whole-cell antibody. This work provides evidence that at least one strain of P. intermedia can invade an oral epithelial cell line and that the type C fimbriae and a cytoskeletal rearrangement are required for this invasion. PMID:9826397

  14. HACIA UN CURRÍCULO FLEXIBLE: SALIDAS INTERMEDIAS Y CURSOS OPTATIVOS

    Directory of Open Access Journals (Sweden)

    Carlos Ortigoza Garcell

    2013-04-01

    Full Text Available Tradicionalmente los currículos de las carreras universitarias se han caracterizado por el predominio de una formación de tipo terminal con una sola salida al mercado laboral. Con el fin de contribuir de manera flexible desde la Formación Laboral y Profesional a que los estudiantes definan su trayectoria formativa, en este trabajo se analizan las ventajas de las salidas intermedias en una carrera universitaria, sin afectar la continuidad de los estudios superiores ni la calidad en la formación del egresado universitario. Estas permiten a los estudiantes obtener un título de nivel medio que los pone en condiciones de salir al mercado laboral con un perfil ocupacional relacionado con la carrera universitaria que estudia. Desde la perspectiva curricular la propuesta realizada se basa en currículos flexibles con salidas intermedias donde pueden ser de gran utilidad los cursos optativos y/o electivos.PALABRAS CLAVE: currículo; salidas intermedias; asignaturas optativasTOWARDS A FLEXIBLE CURRICULUM: INTERMEDIATE EXITS AND OPTIONAL COURSES ABSTRACTTraditionally, the curricula of the university degree courses have been focused on the formation of professionals within the required period for their graduation (5-6 years, with a single exit to the labour market. This research is aimed at contributing, from the Labour and Professional Formation of the students, to the definition of their formative process, according to their needs and interests. This paper also analyses the advantages of the intermediate exits in a university degree course, and how they allow students to obtain a college degree without affecting the continuation of the higher education studies or the quality of the formation of the university graduate. Such reality favours the insertion of the students in the labour market under conditions that suit the qualifications and experience related to the degree they are doing. From the curricular perspective, the proposal is based on

  15. Efecto de lactoferrina bovina en la formación de biofilms en cepas clínicas de E. coli enteroagregativa

    Directory of Open Access Journals (Sweden)

    Cecilia Coveñas

    Full Text Available Objetivos. Determinar el efecto de lactoferrina bovina (bLf en la formación de biofilms en cepas clínicas de Escherichia coli enteroagregativa (EAEC, y si este efecto es independiente del hierro. Materiales y métodos. Se utilizaron dos métodos: (a cualitativo, mediante observación directa por microscopia óptica, y (b cuantitativo, lecturas de los valores de absorbancia mediante lector de ELISA en presencia de bLf en concentraciones de 0,01mg/mL y 1mg/mL, con y sin hierro, y no bLf (control, en 122 cepas de EAEC para el método cuantitativo (60 cepas de niños con diarrea y 62 de niños sanos y 31 cepas para el método cualitativo. Resultados. (a Método cualitativo: se evaluaron 31 cepas, con y sin hierro. Sin hierro la formación de biofilms fue de 77% (24/31 en el grupo control versus 58% (14/31 con bLf de 0,01 mg/mL y 4% (1/31 con 1 mg/mL. Con hierro la formación de biofilms fue 90% (28/31 en el grupo control versus 55% (17/31 con bLf de 0,01 mg/mL y 4% (1/31 a 1 mg/mL. (b Método cuantitativo: sin hierro la absorbancia medida a OD 560 nm del grupo control fue 0,7 ± 0,5 versus 0,4 ± 0,3 con bLf 0,01mg/mL y 0,3 ± 0,2 con bLf de 1 mg/mL (p<0,0001. Esta disminución en presencia de bLf incluso se dio con hierro. Conclusiones. La bLf tiende a disminuir la formación de biofilms, mostrando un efecto inhibitorio en las cepas clínicas de EAEC, este efecto no es hierro-dependiente

  16. La tendencia ambiental urbana en ciudades intermedias. Una propuesta metodológica

    Directory of Open Access Journals (Sweden)

    Pinzón Botero María Victoria

    2011-11-01

    Full Text Available Desde las últim as décadas, es evidente la preocupación m undial por los im pactos ambientales negativos que ponen en riesgo la sostenibilidad urbana y su calidad ambiental. Diferentes estudios exponen las variables más importantes para minim izar estos impactos ambientales, y en este sentido, este trabajo de investigación muestra cómo a partir de un diseño metodológico más sencillo con 27 variables am bientales estratégicas, es posible evaluar la tendencia am biental urbana de ciudades intermedias. Este grupo priorizado de variables trabajadas en el marco de una gestión compartida, orientará el desarrollo de la ciudad hacia una estructura territorial más sostenible, como reto para la planificación ambiental actual y con visión de futuro.

  17. Flujo vaginal. Etiología en una unidad intermedia de salud

    Directory of Open Access Journals (Sweden)

    Luz Marina Alzate

    1987-12-01

    Full Text Available En una serie de 584 mujeres que consultaron por flujo vaginal a una Unidad Intermedia de Salud se halló que la entidad más frecuente era la vaginosis bacteriana (vaginitis inespecífica o por Gardnerella vaginalis con un 34,8% seguida por la candidiasis (9,4% y la tricomoniasis (43%; hubo también infecciones mixtas (7,2%; la candidiasis, la tricomoniasis y las infecciones mixtas, pero no la vaginosis bacteriana, fueron más frecuentes en mujeres embarazadas; se hace énfasis en la facilidad de diagnosticar vaginosis bacteriana en el laboratorio corriente con base en criterios de muy fácil determinación.

  18. Cepa de Trichoderma útil para el tratamiento y/o prevención de infecciones provocadas por hongos pertenecientes al género Verticillium

    OpenAIRE

    Barroso-Albarracín, Juan Bautista; Chaki, Mounira; Mercado-Blanco, Jesús; Valverde-Corredor, Antonio; López García, Antonio Alejandro

    2011-01-01

    La invención se refiere al uso de la cepa de hongos Trichoderma harzianum CECT 2413 para la prevención y/o tratamiento de infecciones de plantas y/o suelos provocadas por hongos fitopatógenos pertenecientes al género Verticíllium, preferiblemente por los patotipos defoliante o no defoliante de Verticillium dahliae, agente causante de la Verticilosis del olivo. Además, la invención propone el uso de esta cepa de hongos en combinación con otros hongos del género Trichoderma.

  19. Paisajes del delito y la inseguridad en las ciudades intermedias

    Directory of Open Access Journals (Sweden)

    Quim Bonastra

    2011-02-01

    Full Text Available Until very recently, phenomena like delinquency were only considered dysfunctions of the system. However, nowadays this phenomenon has become inherent to its way of functioning. This dynamic is influenced by factors such as the increasing polarization of developed economies or the enormous masses of displaced people, due to either economic or political reasons, from developing countries who, in rich countries, often fall in situations of precariousness. In the following text we will see how all these pieces are interrelated in an intermediate city, and we will analyse the fluctuation of violent or intimidating thefts, as well as the most common places where these are committed.Si hasta hace bien poco se había considerado que fenómenos como la delincuencia no eran más que disfunciones del sistema, en el mundo en el que vivimos este fenómeno se ha convertido en inherente a su manera de funcionar. Influyen en esta dinámica factores como la polarización creciente de las economías desarrolladas o las ingentes masas de desplazados, ya sea por razones económicas o políticas, de países en vías de desarrollo que, en los países ricos, caen las más de las veces en la precariedad. En el texto que sigue se verá como se engarzan todas estas piezas en una ciudad intermedia y se analizará la fluctuación de los robos con violencia o intimidación, así como los lugares típicos en los que éstos se cometen.DOWNLOAD THIS PAPER FROM SSRN: http://ssrn.com/abstract=1739302

  20. Impacto del CO2 sobre la densidad celular en seis cepas de microalgas marinas

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    Alberto I. Oscanoa Huaynate

    2015-12-01

    Full Text Available Debido a la gran facilidad con que las microalgas pueden capturar el CO2 del medio ambiente, resulta interesante evaluar la cantidad y tiempo de ingreso de éste a los cultivos masivos, con la fi nalidad de aumentar la densidad celular. El objetivo del presente estudio fue evaluar los tiempos de inyección del mencionado gas, durante la producción de biomasa que conlleve a una mayor densidad celular, evaluando además, la variación del pH sin alterar la calidad del cultivo. A partir de seis cepas obtenidas del Banco de Germoplasma del Instituto del Mar del Perú, se realizaron cultivos tipo batch de 300L en invernadero, el tiempo de cultivo de la fase exponencial donde se realizaron las pruebas fue de tres días. Los datos se procesaron mediante el análisis del parámetro pendiente de la regresión lineal. Los resultados mostraron que la densidad celular es inversamente proporcional al tiempo de inyección de CO2 al cultivo. La mayor densidad celular, en las diferentes cepas, se obtuvo a los 5min, excepto para las cepas Chaetoceros gracilisy Nannochloris maculata, las cuales obtienen la mayor densidad a los 10 y 15min, respectivamente. La variación de pH tendió hacia la acidez, en un rango de 8 a 4, sin alterar la densidad celular, por el contrario, los cultivos permanecieron libres de contaminantes. En conclusión, los resultados permiten establecer tiempos adecuados de inyección del CO2, los cuales fortalecen la fase de crecimiento exponencial aumentando la densidad poblacional en un 30% sobre lo establecido en esta fase.

  1. Caracterización in vitro y producción de inóculo de cepas guatemaltecas de Lepista nuda (Bull.: Fr. Cooke

    Directory of Open Access Journals (Sweden)

    María del Carmen Bran

    2015-11-01

    Full Text Available Lepista nuda es un hongo comestible muy apreciado por su delicado sabor, especialmente en varios países de Europa. En Guatemala, lo consumen personas de las etnias Kaqchikel y Mam, por lo que constituye un alimento ancestral que puede aprovecharse como una alternativa alimenticia y económica. Por tal razón, en este estudio se investigaron cinco cepas nativas de L. nuda y se evaluó su crecimiento in vitro en tres medios de cultivo (agar extracto de malta –AEM–, agar papa dextrosa -APD- y agar Sabouraud -SAB- a dos temperaturas (18 y 26°C, así como la producción de inóculo en granos de trigo, cebada y sorgo. Se determinó que el medio más adecuado para el crecimiento micelial de las cepas fue AEM incubado a 26°C. Todas las cepas presentaron colonias de color blanco a lila, textura algodonosa, bordes irregulares, con hifas de 1 a 4 μm y fíbulas en regular cantidad a abundantes en todos los medios evaluados. En la producción de inóculo, las cepas presentaron mayor velocidad de colonización en los granos de trigo. Se recomienda utilizar AEM para la producción de biomasa y granos de trigo para la producción de inóculo de las cepas evaluadas. 

  2. Polihidroxialcanoatos de cepas de Azospirillum spp. aisladas de raíces de Lycopersicon esculentum Mill. “tomate” y Oryza sativa L. “arroz” en Lambayeque

    Directory of Open Access Journals (Sweden)

    Katty Baca

    2010-01-01

    Full Text Available En este trabajo se determinó la concentración de polihidroxialcanoatos (PH As de cepas de Azospirillum aisladas de raíces de Lycopersicon esculentum Mill. “tomate” y Oryza sativa L. “arroz”, como una alternativa ante la acumulación de plásticos derivados del petróleo. R aíces previamente desinfectadas se sembraron en medio Nfb se misólido, donde las bacterias fijadoras de nitrógeno se reconocieron por una película blanquecina bajo la superficie y el viraje del indicador al azul. El género Azospirillum se identificó en medio rojo de Congo, obteniéndose 96 cepas de A. lipoferum y A. brasilense en tomate y arroz. Se realizó una fermentación discontinua con caldo Azotobacter modificado, alimentando con una solución saturada de ácido málico cada 12 horas y se realizaron tinciones con Sudán Negro B. Se seleccionaron las cepas con mayor nú mero de gránulos de PHAs (en tomate , 18 de A. lipoferum y 2 de A. brasilense y en arroz, 10 de A. lipoferum y 10 de A. brasilense y se cuantificó la biomasa y PHAs. La concentración de PHAs alcanzó 0 . 661 gL - 1 en A. lipoferum KM(T - 73 y 0 . 738 gL - 1 en A. br asilense KM(T - 19. Las cepas de A. lipoferum y A. brasilense aisladas de tomate alcanzaron una mayor concentración de biomasa y PHAs frente a las cepas aisladas de arroz.

  3. Cepas de Campylobacter jejuni resistentes a quinolonas aisladas de humanos, gallinas y pollos

    Directory of Open Access Journals (Sweden)

    Rodolfo Notario

    2011-08-01

    Full Text Available Se compararon 8 aislamientos de Campylobacter jejuni provenientes de humanos con enfermedad diarreica aguda, con 23 aislamientos de cloaca de gallinas y pollos obtenidos de zonas próximas a la ciudad de Rosario, todos resistentes a la ciprofloxacina. Las muestras se sembraron en agar selectivo y se incubaron en microaerofilia a 42 °C. Las colonias se identificaron con el método tradicional. Los aislamientos se conservaron a -70 °C en caldo cerebro corazón con 17% v/v de glicerina. La clonalidad se determinó por RAPD-PCR, utilizando el primer 1254 (Stern NJ. Se interpretaron los aislamientos como clones distintos cuando diferían en una banda de amplificación. Se obtuvieron 5 clones diferentes. Los patrones I, II y V fueron aislados en criaderos industriales de pollos y en humanos (el II también en un establecimiento de gallinas ponedoras de huevos. En un gallinero familiar se obtuvo el patrón I. El patrón III sólo se obtuvo de humanos. El patrón IV se halló en uno de los criaderos pero no en humanos. Se pudo determinar que 93.5% de las cepas se aislaron tanto de animales como de humanos, por lo que se considera posible que la colonización de criaderos con cepas resistentes a los antimicrobianos pudiera ser el origen de la infección de humanos.

  4. Phytochemical Screening and in vitro Activity of Allium cepa. L ...

    African Journals Online (AJOL)

    user

    Department of Applied Science, College of Science and Technology. Kaduna ... Phytochemical analysis of Allium cepa L. ethanolic extract revealed the presence of. Saponins, Tanins, flavonoids and alkaloids while steroids were not detected.

  5. Morphometric and genetic analysis of Arcella intermedia and Arcella intermedia laevis (Amoebozoa, Arcellinida) illuminate phenotypic plasticity in microbial eukaryotes.

    Science.gov (United States)

    Porfírio-Sousa, Alfredo L; Ribeiro, Giulia M; Lahr, Daniel J G

    2017-04-01

    Testate amoebae are eukaryotic microorganisms characterized by the presence of an external shell (test). The shell morphology is used as a diagnostic character, but discordance between morphological and molecular data has been demonstrated in groups of arcellinids (Amoebozoa), one of the principal groups of testate amoebae. Morphology of the test is supposed to differentiate genera and species and it is applied in ecological, monitoring and paleontological studies. However, if phenotype does not reflect genotype, conclusions in these types of studies become severely impaired. The objective of this work is to evaluate the morphometrical and morphological variation of the closely related and morphologically similar taxa Arcella intermedia laevis Tsyganov and Mazei, 2006 and Arcella intermedia (Deflandre 1928) Tsyganov and Mazei, 2006 in nature and in cultured individuals and see how these are correlated with molecular data. Our results demonstrate that phenotypic plasticity in Arcella intermedia make morphological distinctions impossible in both taxa. Arcella intermedia and Arcella intermedia laevis are molecularly identical for SSU rDNA and a mitochondrial molecular marker (NAD9/7). We conclude that morphological techniques alone cannot identify phenotypic plasticity from natural populations. More work is clearly needed to better understand the morphological, morphometric and molecular variability in these organisms. Copyright © 2016 Elsevier GmbH. All rights reserved.

  6. Desarrollo curricular, conciencia ambiental y tecnologia para estudiantes de intermedia: Una investigacion en accion

    Science.gov (United States)

    Rodriguez Ramos, Teresita

    Se llevó a cabo una investigación en acción con los propósitos de 1) documentar las relaciones de las tecnologías de la información y la comunicación en las clases de ciencias de escuela intermedia como elemento de apoyo cuando se aborda el tema ambiental y sus conceptos pertinentes, a partir de las observaciones de la investigadora, así como las entrevistas y diarios reflexivos de los estudiantes de una escuela intermedia en la zona metropolitana, y luego 2) diseñar una unidad instruccional sobre el tema ambiental que integre actividades tecnologías para el curso de ciencias de la escuela intermedia según el modelo PROCIC y las observaciones que hayan iniciado los estudiantes participantes. Finalmente, se plantearon las implicaciones educativas para el currículo del Programa de Ciencias al instrumentar este modelo de unidad mediante PROCIC, e integrado la tecnología y el tema ambiental. Los hallazgos se analizaron y se categorizaron de acuerdo con las preguntas de investigación. El hallazgo principal de la investigación aborda las cuatro relaciones centrales en las que se articula la utilización de las tecnologías y sus aplicaciones en la clase de ciencias. Estas cuatro relaciones que recogen la posición de los estudiantes son: 1) Perspectiva de los estudiantes hacia la tecnología. 2) Participación de los estudiantes en los aspectos docentes. 3) Aprendizaje estudiantil sobre el ambiente, y 4) Conciencia ambiental en relación con la vida diaria. Estas relaciones ponen de manifiesto,cómo se plantea en las implicaciones, la necesidad de más investigación en acción en la sala de clases, la importancia—como tema transversal—de la conciencia ambiental mediante la tecnología al construir conocimientos significativos dentro y fuera de la escuela, asó como, valorar la investigación y la dialogicidad en la sala de clases como actividades que obligan al reexamen de la práctica didáctica en su formas curriculares de objetivos, recursos

  7. Thalidomide has a significant effect in patients with thalassemia intermedia.

    Science.gov (United States)

    Li, YunShuan; Ren, Quan; Zhou, Yali; Li, Pingping; Lin, Wanhua; Yin, Xiaolin

    2018-01-01

    To investigate the effect of thalidomide in patients with thalassemia intermedia. We observed the effect of thalidomide in seven patients with thalassemia intermedia requiring blood transfusion. Four of the patients were transfusion-independent, and three patients were transfusion-dependent. For the four transfusion-independent patients, hemoglobin concentration increased significantly (≥2 g/dl) in three and moderately (1-2 g/dl) in one. After 3 months of treatment, hemoglobin concentration increased 3.2 ± 1.2 g/dl compared to pretreatment. Among the three transfusion-dependent patients, transfusion was terminated after one month of treatment in one patient and decreased >50% in the other two patients, accompanied by an increase in the average hemoglobin concentration. Thalidomide had a significant effect in patients with thalassemia intermedia. Further studies of a larger scale and more rigorous design are warranted.

  8. Efecto de cinco Dosis de Heterorhabditis indica Poinat cepa P2M sobre la broca del café (Hypothenemus hampei Ferrari).

    OpenAIRE

    Yander Fernández Cancio; Marcos Tulio García González; Manuel Rodríguez González

    2015-01-01

    Con el objetivo de evaluar el efecto de Heterorhaditis indica cepa P2M en el manejo de la broca del café (Hypotenemushampei Ferrari) se realizó la investigación en condiciones controladas en el Laboratorio Provincial de Sanidad Vegetal de Sancti Spíritus con cinco dosis del patógeno: 20, 45, 75, 100 y 200 individuos juveniles infestiles/adulto (iji/adulto). Se diseñaron dos experimentos: variante 1 con aplicaciones directa sobre los adultos del insecto en placas Petri con siete repeticiones y...

  9. Seleccion de cepas de rizobios en suelos promisorios para el cultivo de la soya (Glycine max (L Merril en Colombia

    Directory of Open Access Journals (Sweden)

    Sylvester Bradley Rosemary

    1989-06-01

    Full Text Available En el invernadero se evaluó la efectividad de las cepas preseleccionadas de B. japonicum (CIAT 199,209,3778,3874 y 3876 de la recomendada (CIAT51 y una de Rhizobium spp (CIAT 3779. En el suelo Fluventic Haplustoll, de reacción casi neutra, se presentó respuesta altamente significativa a la inoculación. El tratamiento fertilizado con 150 kg de N/ha produjo el mayor rendimiento de N. Las cepas más efectivas fueron CIAT 3778 y 51, que también presentaron el mayor indice de efectividad a la inoculación (IEI. En el suelo Typic Pellustert,de reacción alcalina, se presentó respuesta significativa a la inoculación. El mayor rendimiento de N se obtuvo con las cepas CIAT 3778 y 51, que fueron además estadísticamente iguales a las cepas CIAT 199 y 3876 en cuanto al IEI. En el suelo Arenic Haplustalf, de reacción ligeramente ácida, hubo respuesta altamente significativa a la inoculación y la cepa más efectiva fue la CJAT 199. En el suelo Fluventic Ustropept, de reacci6n ligeramente ácida, se presentaron diferencias significativas entre los tratamientos inoculados. El mayor rendimiento de N se presentó en el tratamiento fertilizado con N. Las mejores cepas fueron CIAT 3778,3779 y 209. En el suelo Vertic Ustropept, de reacción alcalina, se presentó respuesta altamente significativa a la inoculación. En cuanto al rendimiento de N no se encontraron diferencias entre el tratamiento fertilizado con N y las cepas CIAT 51, 209 y CIAT 3778, siendo también las más efectivas en cuanto al IEI, junto oon la cepa CIAT 199. En los suelos Tropentic Haplorthox y Typic Tropudult no se enoontró respuesta a la inoculación.Screening of Rhizobium strains in soils apt for soybean (Glycine max (L Merril cultivation in Colombia. Under greenhouse conditions were evaluated the effectivity of the preselected strains of the preselected strains of B. japonicum (CIAT 199, 209, 3778, 3874 and 3876, in addition to the recommended strain (CIAT 51 and one of

  10. Proteasas extracelulares producidas por bacterias marinas aisladas de aguas contaminadas con efluentes pesqueros

    Directory of Open Access Journals (Sweden)

    Tito Sánchez

    2013-06-01

    Full Text Available Un total de 26 cepas de bacterias marinas con actividad proteolítica fueron aisladas de agua de mar contaminadas con efluentes pesqueros; las mismas que se evaluaron en base al crecimiento y formación de halos de actividad en Agar Marino suplementados con caseína al 1%, pH 8,0 e incubados a 25 ºC por 72 h. Cinco cepas, seleccionadas por presentar los mejores halos de actividad fueron evaluadas a su vez por su crecimiento y producción de proteasas a diferentes concentraciones de NaCl, rangos de temperatura y pH; siendo consideradas finalmente como bacterias halotolerantes, psicrotróficas y alcalófilas moderadas. Estas cepas también fueron evaluadas por su actividad proteolítica específica sobre la caseína, siendo la cepa CM48 (Pseudomonas sp. la que presentó la mejor actividad específica (17,38 U/mg a las 72 horas, y seguidas por las cepas CM45 (Alcaligenes sp. (12,09 U/mg y tres cepas de Aeromonas sp. (CM43, CM44 y CM46 con valores de 12,02; 10,07 y 10,10 U/mg respectivamente.

  11. Irradiation for progressive paraparesis in β-thalassemia intermedia

    International Nuclear Information System (INIS)

    Wilson, J.J.; Hill, M.E.; Crookston, J.H.

    1980-01-01

    This article describes the case history of a patient suffering from β-thalassemia intermedia. The patient was treated with radiation to the thoraic spine (1500 rad in 5 days) and his condition improved. Subsequent tomography indicated the success of this type of irradiation without recourse to laminectomy. (T.I.)

  12. Nucleases from Prevotella intermedia can degrade neutrophil extracellular traps.

    Science.gov (United States)

    Doke, M; Fukamachi, H; Morisaki, H; Arimoto, T; Kataoka, H; Kuwata, H

    2017-08-01

    Periodontitis is an inflammatory disease caused by periodontal bacteria in subgingival plaque. These bacteria are able to colonize the periodontal region by evading the host immune response. Neutrophils, the host's first line of defense against infection, use various strategies to kill invading pathogens, including neutrophil extracellular traps (NETs). These are extracellular net-like fibers comprising DNA and antimicrobial components such as histones, LL-37, defensins, myeloperoxidase, and neutrophil elastase from neutrophils that disarm and kill bacteria extracellularly. Bacterial nuclease degrades the NETs to escape NET killing. It has now been shown that extracellular nucleases enable bacteria to evade this host antimicrobial mechanism, leading to increased pathogenicity. Here, we compared the DNA degradation activity of major Gram-negative periodontopathogenic bacteria, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. We found that Pr. intermedia showed the highest DNA degradation activity. A genome search of Pr. intermedia revealed the presence of two genes, nucA and nucD, putatively encoding secreted nucleases, although their enzymatic and biological activities are unknown. We cloned nucA- and nucD-encoding nucleases from Pr. intermedia ATCC 25611 and characterized their gene products. Recombinant NucA and NucD digested DNA and RNA, which required both Mg 2+ and Ca 2+ for optimal activity. In addition, NucA and NucD were able to degrade the DNA matrix comprising NETs. © 2016 The Authors Molecular Oral Microbiology Published by John Wiley & Sons Ltd.

  13. Circaea x intermedia Ehrh. (Klein heksenkruid) een tuinplant?

    NARCIS (Netherlands)

    Beringen, Ruud

    2001-01-01

    Circaea x intermedia, a very rare taxon in the Netherlands, was found on a new locality on the edge of a woodland near the outskirts of the town of Ede. Growing conditions on the site are not suitable for both parental species ( C. alpina and C. lutetiana). Therefore it is most unlikely the hybrid

  14. Evaluación de la susceptibilidad antimicrobiana de cepas de Salmonella spp. aisladas del beneficio porcino en Colombia / Evaluation of antimicrobial susceptibility of Salmonella spp. strains isolated from pork carcasses on Colombia

    Directory of Open Access Journals (Sweden)

    Paula M. Bermúdez D

    2014-01-01

    Full Text Available Objetivo: el estudio buscó establecer patrones de resistencia antimicrobiana de 155 cepas de Salmonella spp., aisladas de la superficie de canales de cerdo, procedentes de plantas de beneficio en Colombia. Metodología: mediante el método de difusión en disco (Bauer et. Kirby, 1966 se evaluaron nueve antimicrobianos: Amoxicilina y ácido clavulánico (30 ug, Ampicilina (10 ug, Ceftiofur (30 ug, Ciprofloxacina (5 ug, Cloranfenicol (30 ug, Florfenicol (30 ug, Gentamicina (10 ug, Sulfadiazina y Trimetoprim (25 ug y Tetraciclina (30 ug. Resultados: los resultados confirmaron la presencia de cepas multiresistentes de Salmonella spp. de origen porcino, mostrando 30 patrones diferentes de multiresistencia; el más común fue Ampicilina, Amoxicilina, Cloranfenicol, Florfenicol y Tetraciclina en el 16,77% (n=26 de las cepas. Tetraciclina y Florfenicol fueron los antimicrobianos menos efectivos en el 94,84% (n=147 y 47,74% (n=74 de cepas resistentes, respectivamente. Discusión: se deben consolidar los sistemas de monitoreo, y de vigilancia y control de las resistencias antimicrobianas para prevenir, con un enfoque de cadena productiva, la diseminación de cepas multiresistentes en alimentos de origen animal, como parte integral del sistema de gestión de inocuidad de los alimentos en Colombia. - Objetivo: el estudio buscó establecer patrones de resistencia antimicrobiana de 155 cepas de Salmonella spp., aisladas de la superficie de canales de cerdo, procedentes de plantas de beneficio en Colombia. Metodología: mediante el método de difusión en disco (Bauer et. Kirby, 1966 se evaluaron nueve antimicrobianos: Amoxicilina y ácido clavulánico (30 ug, Ampicilina (10 ug, Ceftiofur (30 ug, Ciprofloxacina (5 ug, Cloranfenicol (30 ug, Florfenicol (30 ug, Gentamicina (10 ug, Sulfadiazina y Trimetoprim (25 ug y Tetraciclina (30 ug. Resultados: los resultados confirmaron la presencia de cepas multiresistentes de Salmonella spp. de origen porcino, mostrando

  15. Isolation and characterization of Cepa2, a natural alliospiroside A, from shallot (Allium cepa L. Aggregatum group) with anticancer activity.

    Science.gov (United States)

    Abdelrahman, Mostafa; Mahmoud, Hassan Y A H; El-Sayed, Magdi; Tanaka, Shuhei; Tran, L S

    2017-07-01

    Exploration of new and promising anticancer compounds continues to be one of the main tasks of cancer research because of the drug resistance, high cytotoxicity and limitations of tumor selectivity. Natural products represent a better choice for cancer treatment in comparison with synthetic compounds because of their pharmacokinetic properties and lower side effects. In the current study, we isolated a steroidal saponin, named Cepa2, from the dry roots of shallot (Allium cepa L. Aggregatum group), and determined its structure by using two-dimensional nuclear manganic resonance (2D NMR). The 1 H NMR and 13 C NMR data revealed that the newly isolated Cepa2 compound is identical to alliospiroside A (C 38 H 60 O 12 ) [(25S)-3β-hydroxyspirost-5-en-1β-yl-2-O-(6-deoxy-α-L-mannopyranosyl)-α-L-arabinopyranoside], whose anticancer activity remains elusive. Our in vitro examination of the cytotoxic activity of the identified Cepa2 against P3U1 myeloma cancer cell line showed its high efficiency as an anticancer with 91.13% reduction in P3U1 cell viability 12 h post-treatment. The reduction of cell viability was correlated with the increase in reactive oxygen species levels in Cepa2-treated P3U1 cells, as compared with untreated cells. Moreover, scanning electron microscope results demonstrated apoptosis of the Cepa2-treated P3U1 cells in a time course-dependent manner. The results of our study provide evidence for the anticancer properties of the natural Cepa2/alliospiroside A extracted from shallot plants, and a strong foundation for in-depth investigations to build theoretical bases for cell apoptosis and development of novel anticancer drugs. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  16. AISLAMIENTO DE CEPAS NATIVAS DE TRICHODERMA SPP DE SUELOS HORTICOLAS DEL VALLE DE TOLUCA, COMO BIOCONTROL POTENCIAL DE SCLEROTINIA

    Directory of Open Access Journals (Sweden)

    Hilda G. García-Nuñez

    2012-08-01

    Full Text Available Se evaluó la presencia de Trichoderma en siete localidades en la zona sur del Valle de Toluca, Estado de México. Esta es un área con un alto potencial en la producción de hortalizas. El estudio se dirigió al aislamiento de cepas nativas de Trichoderma a partir de muestras de suelo, identificación de factores fisiográficos, así como las propiedades físicas y químicas del suelo que determinan la ocurrencia de Trichoderma. Se evaluó el potencial de las cepas de Trichoderma para el control de Sclerotinia spp., hongo patógeno causante de la pudrición blanda en lechuga. Se aislaron once cepas, el mayor número de ellas asociadas al tipo de suelo se encontró en las localidades de San Francisco Putla y San Francisco Tetetla. El análisis de regresión logística mostró que no hay una relación entre las propiedades del suelo (materia orgánica y pH y la presencia de Trichoderma. La prueba de Tukey (p

  17. Selección de cepas de Shigella sonnei para el desarrollo de una vacuna efectiva contra la shigellosis

    Directory of Open Access Journals (Sweden)

    Olga M. Martínez

    2008-12-01

    Full Text Available En el Instituto Finlay se desarrolló una metodología de trabajo que contribuyó a la selección de cepas de Shigella sonnei como posibles candidatos vacunales contra la shigellosis. Las cepas investigadas, donadas por el Centro Provincial de Higiene y Epidemiología de Ciudad de La Habana, se caracterizaron según los métodos convencionales. La identificación del serogrupo y serotipo se realizó por aglutinación en láminas portaobjetos con antisueros comerciales; mientras que para el estudio de la susceptibilidad antimicrobiana se utilizó el DIRAMIC 10, un equipo semiautomatizado que proporcionó los resultados 4 horas después de su realización. Se investigó también la presencia de plásmidos de virulencia, por el crecimiento de Shigella spp. en medio de agar Triptona Soya con Rojo Congo al 0,025%, así como la expresión de las proteínas de la membrana externa en SDS-PAGE; para las pruebas de virulencia y potencia se emplearon los modelos animales (modelo ratón-pulmón y Test de Sereny. Los resultados obtenidos con la metodología utilizada permitieron la selección de la cepa de S. sonnei A-04 como la más adecuada para la obtención del posible candidato vacunal.

  18. Indicadores de sensibilidad de una cepa experimental de Lutzomyia longipalpis (Diptera: Psychodidae a tres insecticidas de uso en salud pública en Colombia

    Directory of Open Access Journals (Sweden)

    Catalina Marceló

    2014-12-01

    Conclusión. Se estableció la línea base de la mortalidad frente a tres insecticidas en una cepa sensible del vector L. longipalpis. Estos indicadores permitirán establecer comparaciones con poblaciones del vector expuestas regular o esporádicamente a medidas de control químico y detectar, así, cambios en la sensibilidad de esta especie a los insecticidas.

  19. Caracterización de cepas nativas de Azotobacter spp. y su efecto en el desarrollo de Lycopersicon esculentum Mill. “tomate” en Lambayeque

    Directory of Open Access Journals (Sweden)

    Cynthia Escobar

    2011-01-01

    Full Text Available El objetivo de la presente investigación fue caracterizar y determinar el efecto de cepas nativas de Azotobacter spp. en el desarrollo vegetativo de Lycopersicon esculentum Mill. “tomate”, como una alternativa al uso indiscriminado de fertilizantes químicos. Se tomaron muestras de raíces y suelo rizosférico de hortalizas con las que se realizaron diluciones (10-4 en caldo Ashby-Sacarosa y se incubaron a 30 ºC hasta observar un color amarillo, turbidez y película superficial. El género Azotobacter se identificó en agar mineral sin nitrógeno y Ashby-Benzoato, obteniéndose 96 cepas con una producción de 7.10 a 57.99 mgL-1 de ácido indolacético, 0.13 a 1.64 mgL-1 de nitrógeno fijado como amonio y hasta 1.61 % de eficiencia en la solubilización de roca fosfórica de Bayóvar. Se obtuvo una suspensión celular (108 de cada una de las cuatro cepas con los mayores valores y se inocularon independientemente y en consorcio, así como una combinación con 50 % de urea-100 % de roca fosfórica, en la rizósfera de tomate cv. Río Grande, en un diseño experimental completamente aleatorio. Todas las cepas nativas incrementaron la altura, volumen radicular, materia seca total, parte aérea y radicular frente al testigo absoluto.

  20. Obtención por Ɣ-irradiación de cepas de Saccharomyces cerevisiae tolerantes a condiciones de cultivo rigurosas, para la producción de bioetanol

    Directory of Open Access Journals (Sweden)

    Sylvia Enid Vazquez Zeballos

    2013-01-01

    Full Text Available Con el objetivo de obtener nuevas cepas de levadura capaces de resistir condiciones rigurosas de cultivo se sometió un cultivo fresco de Saccharomyces cerevisiae M522 a Ɣ-irradiación. Se generó una colección de cepas y se evaluó su capacidad de crecimiento a elevadas concentraciones de azúcar y etanol. Se seleccionó una de las cepas y se estudió en ella el efecto de los productos de degradación de la lignina, oligómeros fenólicos metoxilados obtenidos de su despolimerización oxidativa por tratamiento biológico. Se estudiaron también las enzimas involucradas. Todos los cultivos fueron evaluados por absorbancia a 660 nm tras 24 horas de incubación a 37 ˚C. En cuanto a las fracciones fenólicas, se obtuvo el perfil por espectrofotometría UV y se identificaron enzimas laccasa, desmetilasa y lig-peroxidasa.Se obtuvo una cepa (SacSV-10 con las mismas características de cultivo que la M522 en YPD. Se logró cultivar la cepa en un caldo con 10 % de etanol, cepa que toleró el efecto de los productos de degradación de la lignina, así como una concentración de glucosa de 40 g/L, y en condiciones anaerobias se obtuvo una biomasa mayor que para la M522. En conclusión, SacSV-10 es un prometedor candidato para usar en producciones de alcohol a partir de residuos lignocelulósicos.

  1. Proteasas alcalinas de una cepa nativa de Bacillus sp Alcalofílico Proteasas alcalinas de una cepa nativa de Bacillus sp Alcalofílico

    Directory of Open Access Journals (Sweden)

    A. Sáez Vega

    2006-06-01

    Full Text Available Se evaluó el efecto de cuatro fuentes de nitrógeno sobre la actividad enzimática de proteasas alcalinas, secretadas por una cepa nativa de Bacillus sp Alcalofílico, cultivada a diferentes concentraciones de LMF (Licor de Maíz Fermentado. El crecimiento de la cepa no es afectado por los pH de inoculación de 7,0; 8,5 y 9,5; en contraste con la actividad enzimática y producción de proteína verdadera, que tuvieron sus mejores resultados a pH inicial de 8,5. A este pH se evaluaron dos fuentes de nitrógeno orgánico (extracto de levadura y peptona y dos inorgánicos (NH4Cl y NaNO3The effect of four nitrogen sources on the enzymatic activity of alkaline proteases from a wild strain of Alkalophilic Bacillus sp cultivated to different concentrations from CSL (Corn Steep Liquor. was evaluated. The growth of the strain is not affected by pH of inoculation of 7,0, 8,5 and 9,5, in contrast to the enzymatic activity and true protein production, that had their better results to initial pH of 8,5. To this initial pH of 8,5; two organic nitrogen sources (yeast extract and peptone and two inorganic ones were evaluated (NH4Cl and NaNO3. With peptone the best enzymatic activity to a relation appeared to molar C/N between 1 and 2 was found. For the studied interval of % CSL (0,5 to 2% p/v the concentration of the CSL does not affect the enzymatic activity.

  2. La organización de los actores en el desarrollo territorial. El tren de alta velocidad en la ciudad intermedia

    Directory of Open Access Journals (Sweden)

    Jaume Feliu Torrent

    2007-01-01

    Full Text Available Si queremos evaluar el grado de desarrollo local que aporta el Tren de Alta Velocidad (TAV en una ciudad es primordial observar la capacidad de los actores del territorio de organizarse para aprovechar sus recursos endógenos. Mediante la elaboración de una nueva metodología, se propone el estudio del proceso de desarrollo de tres ciudades intermedias con TAV, Lérida (España, Aviñón (Francia y Novara (Italia. En las conclusiones se ponen de manifiesto ciertos problemas propios de la ciudad intermedia en la ejecución de su proyecto local, básicamente la existencia de una relación multiescalar conflictiva y de una débil red de actores locales. La fragilidad de este contexto urbano es debida a la falta de correctos procesos de gobernanza interna y externa.

  3. De Lucrecio a Borges y estaciones intermedias

    OpenAIRE

    Florio, Rubén

    2011-01-01

    Este trabajo intenta mostrar la pervivencia de Lucrecio en unos pocos momentos de la tradición: en el reemplazo de la religión grecolatina por la cristiana, en la renovada convivencia de ambas creencias a partir del Humanismo (con su repercusión en la pintura del Renacimiento), y su reaparición, explícita o silente, en la producción de Jorge Luis Borges. This paper attempts to show the survival of Lucretius in a few moments of the tradition- in the replacement of the Greco-Roman religion f...

  4. Connection of Gnomonia intermedia to Discula betulina and its relationship to other taxa in Gnomoniaceae.

    Science.gov (United States)

    Green, Sarah; Castlebury, Lisa A

    2007-01-01

    Discula betulina is a foliar pathogen on birch (Betula) and Gnomonia intermedia is found on overwintered birch leaves. Perithecia of G. intermedia developed in vitro on colonies of D. betulina isolated from birch tissues in late summer, and single ascospores of G. intermedia consistently developed into colonies similar to D. betulina, producing typical D. betulina conidia. Isolates of D. betulina could be grouped into two mating types, which produced fertile perithecia of G. intermedia when mated with each other. Mycelia from single-ascospore and single-conidial isolates were inoculated onto shoots of downy birch, causing lesions and die-back from which D. betulina was consistently isolated. ITS region ribosomal DNA sequence analysis confirmed the results of the morphological and mating studies, and found that the closest known relatives of G. intermedia/D. betulina are Gnomoniella nana and Sirococcus clavigignenti-juglandacearum. The conclusion from these studies is that D. betulina is the anamorph of G. intermedia.

  5. CLINICAL APPLICATION OF RECOMBINANT ERYTHROPOIETIN IN BETA-THALASSAEMIA INTERMEDIA.

    Science.gov (United States)

    Asadov, Ch; Alimirzoyeva, Z; Hasanova, M; Mammadova, T; Shirinova, A

    2016-06-01

    Research objective is to study the efficacy of recombinant erythropoietin (epoetin alfa) as alternative method of treatment beta-thalassemia intermedia. Study involved 58 patients with beta-thalassemia intermedia (23 women and 35 men). In all observed patients was defined levels of hemoglobin (Hb), red blood cells (RBC), erythrocyte indexes (MCV, MCH, MCHC), hemoglobin fractions (HbA, HbA2, HbF), serum ferritin, serum erythropoietin before and after administrated rEPO. All patients received rEPO during 6 month at the dose - 10000 IU subcutaneously. The majority of patients - 39 (67%) had a good response to rEPO (increase in hemoglobin level more than 20 g/l); 16 patients (28%) had a mean response (increase in Hb 10 - 20 g/l); in 3 (5%) patients occurred poor response to rEPO therapy (increase in Hb intermedia patients there was a statistically significant change in the number of RBC, levels of HbF and sEPO. The evaluation of interdependence between the indices of the baseline sEPO and increased Hb values in patients after rEPO treatment revealed the presence of the reverse direct relationship (r=-0.67). Based on the results, it can be concluded that the use of rEPO in complex therapy of beta-thalassemia intermedia leads to increased levels of Hb and consequently reducing the need for blood transfusions, and accordingly expected to prevent severe complications of blood transfusion (alloimmunization, hypersplenism, iron overload, contamination transmissible infections) facilitating normal growth and development, and a better quality of life.

  6. Susceptibilidad in vitro a los medicamentos anti-tuberculosos de aislados de cepas del complejo Mycobacterium tuberculosis obtenidos a partir de lobos marinos

    Directory of Open Access Journals (Sweden)

    Amelia Bernardelli

    2004-06-01

    Full Text Available Se han hallado cepas de micobacterias aisladas de lobos marinos del Atlántico sur y pertenecen al complejo de Mycobacterium tuberculosis. Los animales se recibieron en las instalaciones del Oceanario Mundo Marino y fueron tratados apropiadamente para su recuperación con la terapia convencional, cuidados intensivos y suplemento alimentario pero no se observó mejoría en su estado general. Se practicaron necropsias en todos los animales y se observaron lesiones extensas compatibles con tuberculosis en pulmones, hígado, bazo y ganglios linfáticos. Para la identificación de las micobacterias, se realizaron pruebas bioquímicas y técnicas de biología molecular con la sonda IS6110. Además, se identificaron todas las cepas como pertenecientes al complejo M. tuberculosis mediante el equipo LCx M. tuberculosis Assay (Abbott Laboratories. El objetivo de este estudio fue determinar in vitro la sensibilidad de las cepas patrón BCG, H37Rv (M. tuberculosis y AN5 (Mycobacterium bovis y la de las siete aisladas de lobos marinos a isoniacida, rifampicina, estreptomicina y etambutol. La concentración inhibitoria mínima (CIM de las drogas antituberculosas se llevó a cabo con el equipo Mycobacterial Growth Indicator Tube (MGIT, BD, Argentina y la microdilución con el ensayo colorimétrico con bromuro de 3-(4-5 dimetiltiazol-2-2,5 difeniltetrazolio. Todos los aislamientos y las cepas de referencia BCG y AN5 se inhibieron con valores CIM de los de H37Rv con buena concordancia entre los resultados obtenidos con ambas técnicas. Los hallazgos permiten sugerir que podrían ser una importante ayuda terapéutica en los lobos marinos con diagnóstico de tuberculosis y evaluar el posible papel sanitario en la prevención y transmisión de la tuberculosis de los animales a los humanos y el trabajo en conjunto.

  7. Development and evaluation of new primers for PCR-based identification of Prevotella intermedia.

    Science.gov (United States)

    Zhou, Yanbin; Liu, Dali; Wang, Yiwei; Zhu, Cailian; Liang, Jingping; Shu, Rong

    2014-08-01

    The aim of this study was to develop new Prevotella intermedia-specific PCR primers based on the 16S rRNA. The new primer set, Pi-192 and Pi-468, increased the accuracy of PCR-based P. intermedia identification and could be useful in the detection of P. intermedia as well as epidemiological studies on periodontal disease. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Resistencia a drogas de segunda línea en cepas peruanas de Mycobacterium tuberculosis multidrogorresistentes

    Directory of Open Access Journals (Sweden)

    Francesca Barletta

    Full Text Available Objetivos. Determinar los perfiles de resistencia de las quinolonas; ciprofloxacina (Cpx, ofloxacina (Ofx, gatifloxacina (Gfx y moxifloxacina (Mfx, y de los inyectables; kanamicina (Km, amikacina (Am y capreomicina (Cm en cepas multidrogorresistente (MDR. Se buscó la presencia de mutaciones en los genes rrs,tlyA y gyrA/B, y su posible asociación con la resistencia a inyectables y quinolonas. Materiales y métodos. En este estudio piloto descriptivo se seleccionaron cepas MDR aisladas durante junio a diciembre de 2004, que fueron criopreservadas en el banco de muestras del Instituto de Medicina Tropical "Alexander von Humboldt" en Lima, Perú. Se determinó la concentración mínima inhibitoria (CMI para Cpx, Ofx, Gfx, Mfx, Km, Am y Cm. Se investigó las mutaciones presentes en los genes rrs, tlyA y gyrA/B a través de un PCR convencional y posterior secuenciamiento de los productos obtenidos. Resultados. Cuatro de los once aislados presentaron resistencia contra los inyectables y en todas se observó una alta CMI; >120 μg/mL para Km y >160 μg/mL para Am y Cm. Solo dos aislados presentaron resistencia a Ofx con un CMI = 4 μg/mL. Los resultados de secuenciamiento sugirieron que la mutación A1401T en rrs podría ser la causa molecular de resistencia a los inyectables; mientras que en este estudio no se halló ninguna mutación en tlyA ni en gyrA/B asociada a resistencia. Conclusiones. Este estudio sugiere una posible asociación entre la mutación en A1401G y la resistencia a los antibióticos inyectables.

  9. Pigment Production by the Edible Filamentous Fungus Neurospora Intermedia

    Directory of Open Access Journals (Sweden)

    Rebecca Gmoser

    2018-02-01

    Full Text Available The production of pigments by edible filamentous fungi is gaining attention as a result of the increased interest in natural sources with added functionality in the food, feed, cosmetic, pharmaceutical and textile industries. The filamentous fungus Neurospora intermedia, used for production of the Indonesian food “oncom”, is one potential source of pigments. The objective of the study was to evaluate the fungus’ pigment production. The joint effect from different factors (carbon and nitrogen source, ZnCl2, MgCl2 and MnCl2 on pigment production by N. intermedia is reported for the first time. The scale-up to 4.5 L bubble column bioreactors was also performed to investigate the effect of pH and aeration. Pigment production of the fungus was successfully manipulated by varying several factors. The results showed that the formation of pigments was strongly influenced by light, carbon, pH, the co-factor Zn2+ and first- to fourth-order interactions between factors. The highest pigmentation (1.19 ± 0.08 mg carotenoids/g dry weight biomass was achieved in a bubble column reactor. This study provides important insights into pigmentation of this biotechnologically important fungus and lays a foundation for future utilizations of N. intermedia for pigment production.

  10. Thalamic Massa Intermedia Duplication in a Dysmorphic 14 month-old Toddler.

    Science.gov (United States)

    Whitehead, Matthew T

    2015-06-01

    The massa intermedia is an inconstant parenchymal band connecting the medial thalami. It may be thickened in various disease processes such as Chiari II malformation or absent in other disease states. However, the massa intermedia may also be absent in up to 30% of normal human brains. To the best of my knowledge, detailed imaging findings of massa intermedia duplication have only been described in a single case report. An additional case of thalamic massa intermedia duplication discovered on a routine brain MR performed for dysmorphic facial features is reported herein.

  11. Draft genome sequence of a Kluyvera intermedia isolate from a patient with a pancreatic abscess

    DEFF Research Database (Denmark)

    Thele, Roland; Gumpert, Heidi; Christensen, Louise B.

    2017-01-01

    The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain...... an efficient tool for monitoring Kluyvera spp. and its role as a reservoir of multidrug resistance. Therefore, this susceptible K. intermedia genome has many characteristics that allow comparison of resistant K. intermedia that might be discovered in the future....

  12. Hepatoprotective effects of Allium cepa (onion) extracts against ...

    African Journals Online (AJOL)

    Liver damage due to paracetamol hepatotoxicity is a major health challenge worldwide. It is against this background that this study was designed to determine the hepatoprotective effects of the increasing dosage of Allium cepa methanolic extracts on paracetamol induced hepatotoxic rats. Fifty-four (54) adult male albino ...

  13. Comparative Hypolipidaemic Effects of Allium cepa, Allium sativum ...

    African Journals Online (AJOL)

    Diabetes mellitus was induced in 108 out of a total of 117 adult Rattus novergicus using 150mg/kg b wt of alloxan monohydrate. Increasing dosages (200, 250 and 300mg/kg bw ip) of A. cepa, A. sativum and Z. officinale aqueous extracts were given to the diabetic rats for six weeks while the control rats got either normal ...

  14. Verkorting van de kweekcyclus bij ui (Allium cepa L.)

    NARCIS (Netherlands)

    Kampen, van J.

    1970-01-01

    With an annual production of about 250 000 to 300 000 tons, onion (Allium cepa L.) is the most important outdoor vegetable crop in the Netherlands. The greater part, namely 70 to 80 %, has to be exported, and is meeting increasing competition. For this reason and in connection with changed growing

  15. Genotoxic effects of industrial wastewater on Allium cepa L.

    African Journals Online (AJOL)

    STORAGESEVER

    2009-05-04

    May 4, 2009 ... However, such tests take too much time and cost a lot of money. ... and cadmium on apical meristem cells of the A. cepa. The effect of the waste .... through respiration is accumulated as organic com- pounds and ... to occur in the cells. ... is naturally likely to influence the cellular energetic events adversely.

  16. Caracterización fenotípica de cepas invasivas de Neisseria meningitidis aisladas en Cuba durante 20 años

    Directory of Open Access Journals (Sweden)

    Isabel Martínez

    2006-04-01

    Full Text Available Se investigaron los marcadores epidemiológicos (serogrupos, serotipos, subtipos, inmunotipos de 429 cepas invasivas, aisladas en Cuba durante 20 años (1982-2002. Basándonos en el comportamiento de la incidencia de la Enfermedad Meningocócica (EM en el período investigado, las cepas se distribuyeron en dos etapas: epidémica y postepidémica. La epidémica, comprendió 279 cepas aisladas entre 1982-1992 y la ostepidémica, incluyó 150 aislamientos pertenecientes al período comprendido entre 1993-2002. Todas se cultivaron en Agar Mueller Hinton con suero fetal bovino (5% y se incubaron 24-48 horas, 37 0C, en atmósfera húmeda con 5% de C02. La identificación de género, especie y serogrupo, se realizó mediante métodos convencionales; para la caracterización de los sero/subtipos e inmunotipos, se utilizó el ensayo inmunoenzimático (ELISA de células enteras con anticuerpos monoclonales. En ambas etapas predominó el serogrupo B (97,90%: epidémica (96,77% y postepidémica (100%. Sin embargo, el serogrupo C (1,43% y cepas no agrupables (1,8%, sólo se observaron en aislamientos de la etapa epidémica. Los otros marcadores prevalentes fueron: serotipo 4 (86,48%, subtipo P1.19,15 (78,32%, inmunotipo L3,7,9 (90,2% , todos mostraron cifras similares en ambos períodos.Predominó el fenotipo B:4:P1.19,15:L3,7,9 (69,69%, aunque, en la etapa postepidémica (77,34%, el porcentaje fue superior al de la etapa epidémica (65,66% (p<0,05; además, en las cepas de este período, se observó una mayor diversidad de asociaciones fenotípicas. Los resultados obtenidos de esta caracterización fenotípica de las cepas de Neisseria meningitidis aisladas de enfermos aporta datos valiosos al estudio, prevención y control exitoso de la EM en Cuba.

  17. Serovariedades y patrones de susceptibilidad a los antimicrobianos de cepas de Salmonella aisladas de alimentos en Cuba Serotypes and antimicrobial susceptibility patterns of Salmonella strains isolated from food in Cuba

    Directory of Open Access Journals (Sweden)

    Yamila Puig Peña

    2011-12-01

    Full Text Available Se describen los serotipos de 178 cepas de Salmonella enterica aisladas de alimentos en diferentes regiones de Cuba entre enero de 2008 y diciembre de 2009, y el patrón de susceptibilidad a los antimicrobianos de 100 aislados seleccionados mediante muestreo por estratos. Se identificaron 20 serovariedades de Salmonella entre las que predominaron S. Enteritidis (23%; S. Agona (13,5% y S. London (11,2%. Del total, 75% de las cepas fueron resistentes o presentaron resistencia intermedia a al menos uno de los fármacos probados, en el siguiente orden, según su frecuencia: tetraciclina (70,7%; ampicilina (22,7% y ácido nalidíxico (14,7%. Se identificaron 10 patrones de resistencia diferentes y predominaron las cepas resistentes o conresistencia intermedia a un fármaco (89,3%. Tres cepas (S. Infantis, S. Derby y S. Enteritidis fueron multirresistentes y una, de S. Enteritidis, dio un resultado no sensible al ácido nalidíxico y la ciprofloxacina. Se destaca la necesidad de extremar la vigilancia sanitaria integrada en el país para el control de la salmonelosis.The serotypes of 178 isolates of Salmonella enterica taken from food in different regions of Cuba between January 2008 and December 2009 were identified, and the antimicrobial susceptibility pattern of 100 selected isolates was determined by strata sampling. A total of 20 Salmonella serotypes were identified, with a predominance of S. Enteritidis (23%, S. Agona (13.5%, and S. London (11.2%. Of all the strains, 75% were resistant or presented intermediate resistance to at least one of the drugs tested, in the following order: tetracycline (70.7%, ampicillin (22.7%, and nalidixic acid (14.7%. Ten different resistance patterns were identified. The most frequent patterns corresponded to strains that were either drugresistant or had intermediate resistance (89.3%. Three strains (identified as S. Infantis, S. Derby, and S. Enteritidis were multiresistant, and one of them, S. Enteritidis, was

  18. Sobre a dispersão de Lutzomyia Intermedia (Diptera, Psychodidae The dispersion of Lutzomyia intermedia (Diptera, Psychodidae

    Directory of Open Access Journals (Sweden)

    Mario B. Aragão

    1987-12-01

    Full Text Available Utilizando um mapa de domínios morfoclimáticos e um de vegetação, foram estudadas as áreas de dispersão da espécie e obtidas as seguintes conclusões: 1 - Lutzomyia intermedia é um flebótomo que ocorre nas florestas que apresentam o seu máximo de desenvolvimento nas encostas úmidas, mas, que ocupam também áreas de relevo pouco movimentadas ou planas. 2 - No Brasil, essas encostas pertencem às serras do Mar e da Mantiqueira e, no noroeste da Argentina, situam-se na Cordilheira dos Andes. 3 - E provável que a espécie exista nas serras florestadas do interior do nordeste brasileiro.Using vegetation and morphoclimactic domain maps the areas of dispersion of Lutzomyia intermedia were studied and the following conclusions were obtained: 1 - L. intermedia is a sandfly of the forests which have their maximum growth at the humid slopes although these forests can also be found in plains and areas with low hills. 2 - In Brazil these slopes are found at the Serras do Mar and Mantiqueira and in Argentina ath the Andes Mountains in the northwest of the country. 3 - This species probably occurs also in the forested mountainous areas of the northeastern region of Brazil.

  19. Expresión de toxinas activas para larvas de mosquito por una cepa nativa de Asticaccaulis excentricus

    Directory of Open Access Journals (Sweden)

    Sergio Ordúz

    2000-02-01

    Full Text Available

    El control de mosquitos con insecticidas biológicos, como las toxinas producidas por especies del género Bacillus ha sido usado ampliamente en muchos países. Sin embargo, la rápida sedimentación de éstas las coloca fuera del alcance de la zona de alimentación de las larvas de mosquito. Con el propósito de resolver este problema se ha propuesto clonar los genes de dichas toxinas en bacterias acuáticas que son capaces de vivir y multiplicarse en la parte superior de la columna de agua, donde se alimentas los mosquitos anofelinos. Se escogieron dos cepas de la bacteria acuática Asticaccaulis excentricus para expresar los genes de la toxina binaria de B. sphaericus y el gen cry11Bb de B. thuringiensis subesp. Medellín clonados en un vector de expresión adecuado. En experimentos de alimentación se encontró que larvas de las especies Culex quinquefasciatus, Aedes aegypti y Anopheles albimanus fueron capaces de sobrevivir en una dieta basada exclusivamente en esta bacteria gram negativa. Las células de A. excentricus recombinante fueron capaces de expresar ambos genes, pero solamente la cepa expresando los genes de la toxina binaria de B. sphaericus fue tóxica para larvas de mosquito. Extractos de proteasas de A. excentricus no degradaron la toxina Cry11Bb, lo que indica que es otro el mecanismo que interfiere con la expresión de la toxicidad de la toxina Cry11Bb en esta bacteria recombinante. Los experimentos de flotación mostraron que A. excentricus recombiante se mantiene en la parte superior de la columna de agua al igual que las cepas nativas, y por más tiempo que las cepas de Bacillus.

  20. Phenotypic polomorphism in indigenous strains of sporothrix schenckii Polimorfismo fenotípico de cepas autóctonas de Sporothrix schenckii

    Directory of Open Access Journals (Sweden)

    Fernando Montoya Maya

    1996-02-01

    Full Text Available

    We studied macroscopic colony findings, sugar assimilation patterns and virulence of 55 Sporothrix schenckij strains obtained from patients with cutaneous sporothrichosis. They were collected during a 10-year period at the Mycology Laboratory, University of Antioquia, School of Medicine, Medellín, Colombia. Pigmentation types and macroscopic morphological characteristics’ were studied on mycosel agar and malt extract. In most cases 2 or 3 colony colors were present In both media. In mycosel agar only 5 strains (9.1% were monochromatic. Pigmentation was very stable in that medium. Eighty five percent of the mycosel agar colonies were beige, brown, pleated or pleated and umbilicated. All strains assimilated D-glucosa, glycerol and D-xylosa. We established 9 patterns of assimilation (blotypes, from A to I In 25 strains. Both pigmented and albino strains were virulent for mice. We emphasize the diversity of our Indigenous strains, and the importance of genotypic characterization and of the correlation studies of phenotypic and genotypic variation with the clinical and geographical patterns of the disease.

    Se presentan los resultados macroscópicos, de asimilación de azúcares y de virulencia de 55 cepas de Sporothrix schenckii aisladas a partir de lesiones de pacientes con esporotricosis cutánea, que consultaron al laboratorio de micología de la Facultad de Medicina de la Universidad de Antioquia en Medellín, Colombia, y que fueron coleccionadas en el transcurso de 10 años. La morfología macroscópica de las colonias y su pigmentación se evaluaron tanto en agar mycosel como en extracto de malta. La mayoría de las cepas en los dos medios presentaban colonias con 2 ó 3 colores diferentes. En mycosel 5 cepas (9.1% fueron monocromáticas y éste fue el medio más estable para definir las

  1. Establecimiento de un medio de cultivo sumergido para una cepa nativa de un hongo Poliporal

    Directory of Open Access Journals (Sweden)

    Peña Serna Carolina

    2006-07-01

    Full Text Available Se evaluó el efecto de dos velocidades de agitación (80 y 100 rpm, dos fuentes de carbono (glucosa y sacarosa y tres pH iniciales (5.5, 6.0 y 6.5 sobre la producción de fenoles totales, la actividad antioxidante, el crecimiento micelial y el consumo de sustrato de una cepa nativa de un hongo perteneciente al orden Poliporal en cultivo sumergido.El mayor crecimiento micelial se obtuvo al día 3 con un valor de 10 g/L a una velocidad de agitación de 80 rpm, sacarosa como fuente de carbono y pH inicial 5.5. La máxima producción de fenoles y actividad antioxidante se presentó este mismo día, alcanzando valores de 0.18 g GAE/L y 0.28 TEAC (mM/mL, respectivamente, para cualquiera de las dos velocidades de agitación evaluadas, con glucosa como fuente de carbono y con pH inicial 6.0 y/o 6.5 para fenoles y pH inicial 5.5 para actividad antioxidante.Palabras claves: actividad antioxidante, crecimiento micelial, cultivo sumergido, fenoles.

  2. IDENTIFICACIÓN DE GENES CANDIDATOS DE PATOGENICIDAD EN LA INTERACCIÓN DE LA CEPA CENICAFE 9501 CON EL NEMÁTODO DEL NUDO RADICAL Meloidogyne spp. IDENTIFICATION OF PATHOGENIC CANDIDATES GENES IN THE INTERACTION OF THE CENICAFE 9501 STRAIN WITH THE ROOT KNOT NEMATODE Meloidogyne spp.

    Directory of Open Access Journals (Sweden)

    Nadya Lorena Cardona Bustos

    2008-12-01

    Full Text Available En 1997 se registró por primera vez el aislamiento de un hongo Hyphomycete de suelos de Chinchiná (Caldas, Colombia atacando huevos y otros estadios biológicos de las especies de nematodos fitoparásitos Meloidogyne incognita y M. javanica provenientes de lotes comerciales con café. Debido a la imposibilidad de clasificarlo taxonómicamente en los géneros actuales, este aislamiento se ha denominado temporalmente como CENICAFE 9501. Dado su potencial como biocontrolador, se propuso identificar genes candidatos involucrados en el proceso de patogenicidad de huevos de Meloidogyne. Con este fin se construyeron librerías diferenciales mediante el método de hibridación sustractiva. La secuenciación de 188 clones obtenidos permitió identificar 80 unigenes, de los cuales el mayor porcentaje correspondió a secuencias sin homología (32%, seguidas por genes candidatos a funciones de patogénesis (22%, transporte celular (17%, síntesis de proteínas (11% y en menor proporción aquellos involucrados con transcripción y metabolismo primario (18%. Dentro de aquellos genes que contienen marcos de lectura con homología a proteínas que intervienen en la patogenicidad se encuentran una peptidasa, un receptor para sitios de ubiquitinación, una deubiquinasa, una ubiquinona oxidoreductasa, proteína relacionada con la degradación de pared celular, glicosil hidrolasa e hidroxilasa de ácidos grasos, asi como una serin proteasa. Se hace necesaria la validación de las funciones putativas de estos genes candidatos con el fin de incrementar el conocimiento básico de la fisiología de este hongo con potencial biorregulador.In 1997 it was reported for the first time from soil isolation of a Hyphomycete fungus from Chinchiná ( Caldas, Colombia , attacking eggs and other biological stages of the plant parasite nematodes Meloidogyne incognita and M. javanica, from commercial coffee plots. Due to the impossibility to classify it taxonomically under current

  3. SYNCHROTRON X-RAY ABSORPTION-EDGE COMPUTED MICROTOMOGRAPHY IMAGING OF THALLIUM COMPARTMENTALIZATION IN IBERIS INTERMEDIA

    Science.gov (United States)

    Thallium (TI) is an extremely toxic metal which, due to its similarities to K, is readily taken up by plants. Thallium is efficiently hyperaccumulated in Iberis intermedia as TI(I). Distribution and compartmentalization of TI in I. intermedia is highes...

  4. Chair-side detection of Prevotella Intermedia in mature dental plaque by its fluorescence.

    Science.gov (United States)

    Nomura, Yoshiaki; Takeuchi, Hiroaki; Okamoto, Masaaki; Sogabe, Kaoru; Okada, Ayako; Hanada, Nobuhiro

    2017-06-01

    Prevotella intermedia/nigrescens is one of the well-known pathogens causing periodontal diseases, and the red florescence excited by the visible blue light caused by the protoporphyrin IX in the bacterial cells could be useful for the chair-side detection. The aim of this study was to evaluated levels of periodontal pathogen, especially P. intermedia in clinical samples of red fluorescent dental plaque. Thirty two supra gingival plaque samples from six individuals were measured its fluorescence at 640nm wavelength excited by 409nm. Periodontopathic bacteria were counted by the Invader PLUS PCR assay. Co-relations the fluorescence intensity and bacterial counts were analyzed by Person's correlation coefficient and simple and multiple regression analysis. Positive and negative predictive values of the fluorescence intensities for with or without P. intermedia in supragingival plaque was calculated. When relative fluorescence unit (RFU) were logarithmic transformed, statistically significant linear relations between RFU and bacterial counts were obtained for P. intermedia, Porphyromonas gingivalis and Tannerella forsythia. By the multiple regression analysis, only P. intermedia had statistically significant co-relation with fluorescence intensities. All of the fluorescent dental plaque contained P. intermedia m. In contrast, 28% of non-fluorescent plaques contained P. intermedia. To check the fluorescence dental plaque in the oral cavity could be the simple chair-side screening of the mature dental plaque before examining the periodontal pathogens especially P. intermedia by the PCR method. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization.

    Science.gov (United States)

    Masakiyo, Yoshiaki; Yoshida, Akihiro; Shintani, Yasuyuki; Takahashi, Yusuke; Ansai, Toshihiro; Takehara, Tadamichi

    2010-06-01

    Prevotella intermedia and Prevotella nigrescens, which are often isolated from periodontal sites, were once considered two different genotypes of P. intermedia. Although the genomic sequence of P. intermedia was determined recently, little is known about the genetic differences between P. intermedia and P. nigrescens. The subtractive hybridization technique is a powerful method for generating a set of DNA fragments differing between two closely related bacterial strains or species. We used subtractive hybridization to identify the DNA regions specific to P. intermedia ATCC 25611 and P. nigrescens ATCC 25261. Using this method, four P. intermedia ATCC 25611-specific and three P. nigrescens ATCC 25261-specific regions were determined. From the species-specific regions, insertion sequence (IS) elements were isolated for P. intermedia. IS elements play an important role in the pathogenicity of bacteria. For the P. intermedia-specific regions, the genes adenine-specific DNA-methyltransferase and 8-amino-7-oxononanoate synthase were isolated. The P. nigrescens-specific region contained a Flavobacterium psychrophilum SprA homologue, a cell-surface protein involved in gliding motility, Prevotella melaninogenica ATCC 25845 glutathione peroxide, and Porphyromonas gingivalis ATCC 33277 leucyl-tRNA synthetase. The results demonstrate that the subtractive hybridization technique was useful for distinguishing between the two closely related species. Furthermore, this technique will contribute to our understanding of the virulence of these species. 2009 Elsevier Ltd. All rights reserved.

  6. Protocolos molecular y celular para elaborar y determinar una cepa vacunal de salmonella enterica serotipo enteriditis 82139 y propuesta de tipos de biorreactores para su produccion

    OpenAIRE

    Villanueva Alva, Liliana Verónica

    2016-01-01

    Se presenta Protocolos Molecular y Celular para Elaborar y Determinar una cepa vacunal de Salmonella entérica Serotipo Enteritidis 82139 y Propuesta de Tipos de Biorreactores para su Producción. El Diseño Factorial permitió en un inicio los diseños y selección para la construcción de cepas mutantes hasta la validación estadística en la determinación de la función génica de dsbA, dle y dlp con respecto a la capacidad de movilidad y de invasión intracelular bacteriana a partir de la determinaci...

  7. Tipificación molecular por PCR-RFLPS de cepas trypanosoma sp. aisladas en campo y evaluación de ganados de la Orinoquia colombiana

    Directory of Open Access Journals (Sweden)

    Elizabeth Regina Cassalett Bustillo

    2006-07-01

    correspondieron a la cepa Trypanosoma theileri en un 80%, y un 20% a la cepa Trypanosoma vivax. Los resultados que se obtuvieron del estudio morfométrico en las especies T. vivax y T. evansi del Banco de Germoplasma de Corpoica, mostraron que el T. vivax es un parásito con mayor longitud (20-25 μm que el T. evansi (18-19 μm, aunque el flagelo libre de este último siempre fue mayor (7-9 μm al igual que el diámetro de su núcleo. En ambos parásitos la posición del núcleo fue central y la posición del quinetolasto terminal.

  8. [Genetic relationship analysis of Ephedra intermedia from different habitat in Gansu by ISSR analysis].

    Science.gov (United States)

    Zhu, Tian-Tian; Jin, Ling; Du, Tao; Cui, Zhi-Jia; Zhang, Xian-Fei; Wu, Di

    2013-09-01

    To investigate the genetic relationship of Ephedra intermedia from different habitats in Gansu. The genetic diversity and genetic relationship of E. intermedia from different habitats in Gansu were studied by ISSR molecular marker technique. Twelve ISSR primers were selected from 70 ISSR primers and used for ISSR amplification. Total 112 loci were amplified, in which 81 were polymorphic loci, the average percentage of polymorphie bands (PPB) was 72.32%. Clustering results indicated that the wild species and cultivating species were clustered into different group. The wild species, which had closer distance, were clustered into a group. E. intermedia of different habitats in Gansu have rich genetic diversities among species, it is the reason that E. intermedia has strong adaptability and wide distribution. Further, the genetic distance of E. intermedia is associated with geographical distance, the further distance can hinder the gene flow.

  9. Efecto del medio de soporte en la estabilidad biológica de dos cepas de Frankia aisladas de Alnus acuminata H. B. K.

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    Ana María Rey

    Full Text Available Alnus acuminata H. B. K. es una especie arbórea que puede ser incorporada en sistemas silvopastoriles, ya que tiene la capacidad de crecer en suelos marginados y contribuir a la conservación de la biodiversidad y al mejoramiento del suelo; lo cual se relaciona con la incorporación de hojarasca, el efecto de sombra, la retención de humedad, el reciclaje de nutrientes y la fijación de nitrógeno atmosférico, debido a su asociación simbiótica con el actinomiceto Frankia. La utilización de este como inoculante es una tecnología que requiere un profundo análisis de su comportamiento en condiciones controladas. Por otra parte, para la comercialización de los biofertilizantes es importante conservar su calidad el mayor tiempo posible, de lo cual depende la aceptación del producto en la cadena productiva. Por ello, se realizó una investigación con el objetivo de evaluar la estabilidad biológica de las cepas nativas de Frankia (Aan17 y Aac49 con diferentes proporciones sustrato:cepa, así como el efecto del alamacenamiento a 4 °C. Las cepas fueron tolerantes a los cambios del pH y al almacenamiento en condiciones de refrigeración. Se concluye que los inoculantes se deben almacenar a 4 °C durante 120 días, con una proporción de 80:20 (inoculante:sustrato en Aan17 y 60:40 en Aac49. En estos sustratos la proteína microbial se mantuvo por encima de 0,65 mg/mL en Aan17 y 0,7 mg/mL en Aac49.

  10. Beta-thalassemia intermedia associated with moyamoya syndrome.

    Science.gov (United States)

    Göksel, Basak Karakurum; Ozdogu, Hakan; Yildirim, Tulin; Oğuzkurt, Levent; Asma, Suheyl

    2010-07-01

    Moyamoya syndrome (MMS) is a progressive disorder. We report a 19-year-old boy with beta-thalassemia who presented with a left hemiparesis. Brain MRI showed old middle cerebral artery and left frontal subcortical white matter infarcts. Brain magnetic resonance angiography and digital subtraction angiography revealed occlusion of the bilateral internal carotid arteries with a rich network of basal collateral vessels. To our knowledge this is the third report of beta-thalassemia intermedia and MMS, and the first report of a patient in Turkey. It emphasizes the potential for cerebral infarct due to anemia, protein S and thrombocytosis.

  11. Biomodelo para la evaluación de cepas atenuadas como candidatos vacunales contra el cólera humano. I. Estudio de la virulencia, capacidad de colonización y adherencia a la mucosa intestinal

    Directory of Open Access Journals (Sweden)

    Reynaldo Oliva

    2008-04-01

    Full Text Available El cólera continúa siendo en muchos países un problema para la salud humana, manteniéndose como una enfermedad epidémica o endémica que afecta tanto a niños como adultos y causa la muerte en casos no tratados. Una vacuna viva oral contra esta enfermedad puede ser la solución. En el presente trabajo se seleccionó y aplicó un biomodelo para la evaluación de cepas atenuadas genéticamente de Vibrio cholerae como candidatas vacunales contra el cólera. La virulencia, capacidad de colonización y adherencia a la mucosa intestinal de las cepas fueron evaluadas mediante el uso de ratones neonatos de 2 a 4 días de nacidos de la línea Balb/c, con un peso entre 1,5-2 g. Los resultados obtenidos con este biomodelo demostraron que las cepas atenuadas genéticamente son no virulentas, colonizan y se adhieren a la mucosa intestinal. Se concluye que el biomodelo utilizado permite la evaluación y selección de cepas candidatas para vacunas vivas orales contra el cólera.

  12. Optimización de la producción y calidad en cebolla cabezona (Allium cepa mediante el balance nutricional con magnesio y micronutrientes (B, Zn y Mn, Valle Alto del Río Chicamocha, Boyacá

    Directory of Open Access Journals (Sweden)

    Gómez Manuel Iván

    2007-12-01

    Full Text Available

    La ausencia de magnesio y micronutrientes (B, Zn y Mn en planes de fertilización limita el balance nutricional en cebolla, debido a que dichos elementos regulan la expresión del potencial productivo del cultivo y la calidad del producto cosechado. La presente investigación fue realizada en un suelo del Distrito de Riego del Alto Chicamocha (DRAC, clasificado taxonómicamente como Sulfic Endoaquept, familia muy fina sobre orgánica, caolinítica, semiactiva, isomésica (fase ácida, moderadamente profunda. Los resultados mostraron respuestas favorables a la fertilización complementaria específica con 70 kg·ha- 1 de MgO (micromagnesio granulado 40% MgO, donde se reportaron incrementos de 50 % en el rendimiento de primera, mejora en la gravedad específica con diferencias altamente significativas respecto al solo manejo NPK, y se identificó una mayor eficiencia en la absorción foliar del P estableciéndose una relación positiva Mg/P. Respecto al comportamiento de micronutrientes se presentó una interacción positiva Mn·Zn con 4 kg·ha-1 Mn (microman-sulfato de manganeso granulado, 20% Mn y 3,5 kg·ha-1 Zn (microzinc-sulfato de zinc granulado 22% Zn, expresada por incrementos superiores al 18% (P< 0,01 en la producción de cebolla categoría primera. Respecto al manejo NPKMg, las mejoras se observaron directamente en el componente de calidad (diámetro ecuatorial, gravedad específica, características que optimizan la rentabilidad del cultivo; igualmente, se estableció una relación P/Zn importante para el desarrollo, la producción y el manejo nutricional del cultivo. El B no presentó un efecto significativo en las variables evaluadas debido a las altas concentraciones nativas en medios ácidos existentes en este tipo de suelos.

  13. La Ciénaga de Córcoles: un área de sedimentación lacustre-palustre en el Mioceno (Aragoniense inferior-medio) de la Depresión Intermedia (Guadalajara)

    OpenAIRE

    Torres Pérez-Hidalgo, Trinidad José

    1986-01-01

    Las ciénagas de Córcoles son depósitos lacustres-palustres del terce Ciclo Neógeno de la Depresión Intermedia (Aragoniense inferior medio). Estuvieron distalmente relacionadas con el abanico aluvial, aunque rodeadas de un paisaje árido. las concentraciones singenéticas de metales sugiere que también tuvieron aportes de aguas termales.

  14. Carotenogénesis de cinco cepas del alga Dunaliella sp. (Chlorophyceae aisladas de lagunas hipersalinas de Venezuela

    Directory of Open Access Journals (Sweden)

    Miguel Guevara

    2005-09-01

    Full Text Available Se realizaron cultivos discontinuos (medio Algal con 0.5 mM de NaNO3 y 27% de NaCl de cinco cepas de Dunaliella sp., aisladas de diferentes lagunas hipersalinas de Venezuela (Araya, Coche, Peonía, Cumaraguas y Boca Chica y una cepa de referencia (Dunaliella salina LB1644. Los bioensayos se mantuvieron a 25 ± 1 °C con aireación constante, fotoperiodo 12:12 y dos intensidades luminosas (195 y 390 µE.m-2.s-1 durante 30 días. El crecimiento celular se determinó diariamente mediante conteo celular en cámara de Neubaüer. La clorofila a y los carotenoides totales se analizaron al final del ensayo. Las mayores densidades celulares correspondieron a los ensayos de menor intensidad luminosa. La cepa que alcanzó la mayor densidad celular fue la aislada de Boca Chica (8 x10(6 y 2.5 x10(6 cel.ml-1 a 195 y 390 µE.m-2.s-1, respectivamente. El incremento de la intensidad luminosa en los cultivos produjo una disminución significativa de las tasas de crecimiento en todas las cepas. Los carotenoides totales por volumen fueron mayores a 390 µE.m-2 .s-1; siendo las cepas de referencia LB1644, Coche y Araya las que produjeron mayor cantidad (38.4; 32.8 y 21.0 µg.ml-1, respectivamente. El contenido de carotenoides totales por célula en los dos tratamientos fue significativamente diferente, obteniéndose la mayor concentración a 390 µE.m-2.s-1. Las cepas LB1644 y Coche fueron las que produjeron los valores más altos de carotenos (137.14 y 106.06 pg.cel-1, respectivamente. La cepa LB1644 presentó la mayor relación carotenoides totales:clorofila a (20:1 a 195 µE.m-2.s-1, mientras que en la cepa Coche no se evidenciaron diferencias significativas entre las dos intensidades (15:1. El resto de las cepas mostraron relaciones inferiores a uno. Nuestros resultados sugieren que las cepas Coche y Araya pueden ser potencialmente utilizadas en la biotecnología de producción de carotenoides.Carotenogenesis of five strains of the algae Dunaliella sp

  15. Caracterización de dos sistemas de abanicos aluviales húmedos en el Terciario de la Depresión Intermedia (Cuenca-Guadalajara)

    OpenAIRE

    Torres Pérez-Hidalgo, Trinidad José

    1986-01-01

    En este trabajo se comparan las características de dos abanicos aluviales del Primer Ciclo Neógeno (Ageniense superior-Aragoniense inferior) de la Depresion Intermedia. Estos abanicos tienen una serie de características comunes como: área fuente en la Cordillera Ibérica, períodos alternantes de funcinamiento de canales de baja y alta sinuosidad e interdigitación dista1 con depósitos lacustres de yesos bioturbados. Se diferencian en la orientación de paleocorrientes y presenc...

  16. Caracterización de dos sistemas de abanicos aluviales húmedos en el Terciario de la Depresión Intermedia (Cuenca-Guadalajara)

    OpenAIRE

    de TORRES, T.; ZAPATA, J. L.

    1986-01-01

    En este trabajo se comparan las características de dos abanicos aluviales del Primer Ciclo Neógeno (Ageniense superior-Aragoniense inferior) de la Depresion Intermedia. Estos abanicos tienen una serie de características comunes como: área fuente en la Cordillera Ibérica, períodos alternantes de funcinamiento de canales de baja y alta sinuosidad e interdigitación distal con depósitos lacustres de yesos bioturbados. Se diferencian en la orientación de paleocorrientes y presencia en uno de ellos...

  17. Mycelial pellet formation by edible ascomycete filamentous fungi, Neurospora intermedia.

    Science.gov (United States)

    Nair, Ramkumar B; Lennartsson, Patrik R; Taherzadeh, Mohammad J

    2016-12-01

    Pellet formation of filamentous fungi in submerged culture is an imperative topic of fermentation research. In this study, we report for the first time the growth of filamentous ascomycete fungus, Neurospora intermedia in its mycelial pellet form. In submerged culture, the growth morphology of the fungus was successfully manipulated into growing as pellets by modifying various cultivation conditions. Factors such as pH (2.0-10.0), agitation rate (100-150 rpm), carbon source (glucose, arabinose, sucrose, and galactose), the presence of additive agents (glycerol and calcium chloride) and trace metals were investigated for their effect on the pellet formation. Of the various factors screened, uniform pellets were formed only at pH range 3.0-4.0, signifying it as the most influential factor for N. intermedia pellet formation. The average pellet size ranged from 2.38 ± 0.12 to 2.86 ± 0.38 mm. The pellet formation remained unaffected by the inoculum type used and its size showed an inverse correlation with the agitation rate of the culture. Efficient glucose utilization was observed with fungal pellets, as opposed to the freely suspended mycelium, proving its viability for fast-fermentation processes. Scale up of the pelletization process was also carried out in bench-scale airlift and bubble column reactors (4.5 L).

  18. Uveítis intermedia: Experiencias terapéuticas Intermediate uveitis: Therapeutic experiences

    Directory of Open Access Journals (Sweden)

    Sandra M. Borges Pérez

    2001-12-01

    Full Text Available Se realizó un estudio descriptivo prospectivo a 50 ojos (41 pacientes con el diagnóstico de uveítis intermedia durante un período de 3 años. Esta patología fue más frecuente en la mujer (58 %, en edades jóvenes (25 a 45 años y en plena actividad laboral. Se presentan complicaciones y dentro de ellas el edema macular cistoide constituye la causa que más afecta la visión. La mejoría de la agudeza visual fue más notable en los pacientes bajo régimen de tratamiento médico acompañado de tratamiento quirúrgico (40 %. El 50 % de los pacientes presentaron recidivas.A descriptive and prospective study of 50 eyes (41 patients with the diagnosis of intermediate uveitis was conducted during 3 years. This pathology was more frequent in women (58 %, in young patients (25-45 years old and among workers. Among the complications, the cystoid macular edema was the main cause of vision affections. The improvement of visual acuity was more remarkable in patients under medical and surgical treatment (40 %. 50 % of the patients had relapses.

  19. The pars intermedia: an anatomic basis for a coordinated vascular response to female genital arousal.

    Science.gov (United States)

    Shih, Cheryl; Cold, Christopher J; Yang, Claire C

    2013-06-01

    The pars intermedia is an area of the vulva that has been inconsistently described in the literature. We conducted anatomic studies to better describe the tissues and vascular structures of the pars intermedia and proposed a functional rationale of the pars intermedia in the female sexual response. Nine cadaveric vulvectomy specimens were used. Each was serially sectioned and stained with hematoxylin and eosin and Masson's trichrome. Histologic ultrastructural description of the pars intermedia. The pars intermedia contains veins traveling longitudinally in the angle of the clitoris, supported by collagen-rich stromal tissues. These veins drain the different vascular compartments of the vulva, including the clitoris, the bulbs, and labia minora; also, the interconnecting veins link the different vascular compartments. The pars intermedia is not composed of erectile tissue, distinguishing it from the erectile tissues of the corpora cavernosa of the clitoris as well as the corpus spongiosum of the clitoral (vestibular) bulbs. The venous communications of the pars intermedia, linking the erectile tissues with the other vascular compartments of the vulva, appear to provide the anatomic basis for a coordinated vascular response during female sexual arousal. © 2012 International Society for Sexual Medicine.

  20. Control of postharvest Botrytis fruit rot of strawberry by volatile organic compounds of Candida intermedia.

    Science.gov (United States)

    Huang, R; Li, G Q; Zhang, J; Yang, L; Che, H J; Jiang, D H; Huang, H C

    2011-07-01

    A study was conducted to identify volatile organic compounds or volatiles produced by Candida intermedia strain C410 using gas chromatography-mass spectrometry, and to determine efficacy of the volatiles of C. intermedia in suppression of conidial germination and mycelial growth of Botrytis cinerea and control of Botrytis fruit rot of strawberry. Results showed that, among 49 volatiles (esters, alcohols, alkenes, alkanes, alkynes, organic acids, ketones, and aldehydes) identified from C. intermedia cultures on yeast extract peptone dextrose agar, two compounds, 1,3,5,7-cyclooctatetraene and 3-methyl-1-butanol, were the most abundant. Synthetic chemicals of 1,3,5,7-cyclooctatetraene; 3-methyl-1-butanol; 2-nonanone; pentanoic acid, 4-methyl-, ethyl ester; 3-methyl-1-butanol, acetate; acetic acid, pentyl ester; and hexanoic acid, ethyl ester were highly inhibitory to conidial germination and mycelial growth of B. cinerea. Inhibition of conidial germination and mycelial growth of B. cinerea by volatiles of C. intermedia was also observed. Meanwhile, results showed that incidence and severity of Botrytis fruit rot of strawberry was significantly (P intermedia cultures or C. intermedia-infested strawberry fruit. These results suggest that the volatiles of C. intermedia C410 are promising biofumigants for control of Botrytis fruit rot of strawberry.

  1. Gravi-photomorphogenesis of the moss Pottia intermedia protonemata

    Science.gov (United States)

    Demkiv, O. T.; Kyjak, N. Y.; Khorkavtsiv, Y. D.; Kit, N. A.

    The protonemata development proceeds in the process of gradual differentiation of growing apical cells and intercalar cells the shortened lateral branches of the latters being transformed into three-dimensional gametophore buds (Demkiv et al., 1991). Normal course of plant development needs favourable external conditions. Sometimes, however, external environment agents can accelerate the development of organism. So, apical protonema cells of darkgrown gravitropic P. intermedia differentiate gametophore-buds in light of low intensity (Ripetskyj, 1999). We investigate the influence of gravistimulation on bud formation in haploid and diploid P. intermedia protonema. Diploid protonema was found to react on light weaker than haploid one. Under the influence of light the darkgrown apical cells and lateral branches of haploid protonema were directly transformed into buds, while in diploid protonema at first the formation of bundles of rhizoid type filaments takes place on the tips of caulonema and buds appeared in center of such bundles. The participation of gravity in gametophore bud formation was assessed by clinorotating protonema in darkness. Being illuminated such protonema also developed buds quickly the latters being formed along all stolon. It can be suggested that at 1g the growth zone of apical cells actively attract inductors of bud formation. During clinorotation the inductors probably are transferred much more slower than under stationary state and that is why the buds arised not only at the tips of stolons but along all their length. It is known that gametophore bud formation can be stimulated by exogenous phytohormones. As M. Bopp (1980) has shown, that kinetin selectively promotes bud formation on moss protonema. Our observations have shown 0,5 -- 50 μ M of kinetin stimulate the bud formation on diploid aposporic protonema much more effectively that on haploid one. It can be concluded that the amount of endogenous cytokinins in haploid protonemal cells is

  2. Estudios de Osmoiniciacion de semillas de cebolla de bulbo (Allium cepa L. Osmopriming studies in bulb onion seed (Allium cepa L.

    Directory of Open Access Journals (Sweden)

    Arjona Díaz Harvey

    1998-12-01

    Full Text Available El presente trabajo se realizó durante el segundo semestre de 1996 y el primer semestre de 1997. Se dividió en dos etapas: la primera se realizó en los laboratorios de Fisiología Vegetal de la Universidad Nacional de Colombia, Facultad de Agronomía, en donde se establecieron los tratamientos de osmoiniciación; la segunda se realizó en los invernaderos del Centro de Investigaciones y Asesorías Agroindustriales de la Universidad Jorge Tadeo Lozano, en donde se establecieron los semilleros. En el ensayo se evaluaron 18 tratamientos de osmoiniciación utilizando PEG (6000 y KH2P04 en semillas de cebolla de bulbo (Allium cepa L., con potenciales osmóticos de -0,5, -1,0 y -1,5 MPa, durante 5,10 y 15 días de imbibición. El diseño utilizado fue completamente al azar con arreglo factorial 2x3x3 y cuatro replicaciones. Se midieron las variables: 1 Porcentaje de germinación a los 5 días y a los 10 días, 2 porcentaje de emergencia a los 12 días, a los 17 días y a los 21 días, 3 Longitud de raíces, 4 Altura de planta, 5 Número de hojas, 6 Diámetro del bulbo, 7 Peso fresco y 8 Peso Seco. Se obtuvo mejor respuesta con el uso de KH2P04 en los porcentajes de germinación, en los porcentajes de emergencia y en la longitud de raíces.
    This trial was divided in two steps: the first step was conducted in the College of Agriculture, National University of Colombia, where the osmopriming treatment of onion seeds was conducted. The second step was carried out at the Research Center of the Jorge Tadeo Lozano University. Eighteen treatments of osmopriming were proved, by using PEG (6000 and KH2P04 with bulb onion seed (Allium cepa L.. Solutions with three osmotic potentials -0.5, -1.0 and -1.5 MPa were used and seeds were embebed for 5, 10 and 15 days. A Completely Randomized design with a factorial arrangement of 2x3x3 was used. The experiment was replicated four times. The variables measured wereo: 1 Percentage of germination 5 and 10 days

  3. Paraspinal extramedullary hematopoiesis in patients with thalassemia intermedia.

    Science.gov (United States)

    Haidar, Rachid; Mhaidli, Hani; Taher, Ali T

    2010-06-01

    Ineffective erythropoiesis in patients with thalassemia intermedia drives extramedullary hematopoietic tumor formation in several parts of the body. Paraspinal involvement has received increasing attention due to the associated morbidity secondary to spinal cord compression. Although the history and physical examination may help narrow the differential diagnosis, radiographic imaging remains essential to confirm the existence of hematopoietic tissue. Characteristic appearance has been observed mainly on magnetic resonance imaging. Several treatment options have been described, including transfusion therapy, laminectomy, radiotherapy, and the use of fetal hemoglobin inducing agents that decrease the hematopoietic drive. However, the ideal management scheme remains controversial. Until large prospective trials evaluate the efficacy and safety of the available treatment options, both in single and in combination therapy, an individualized approach should be entertained.

  4. Suppression of linalool acetate production in Lavandula x intermedia.

    Science.gov (United States)

    Desautels, Amy; Biswas, Kamal; Lane, Alexander; Boeckelmann, Astrid; Mahmoud, Soheil S

    2009-11-01

    Linalool acetate, one of the major constituent of several essential oils, is heat-labile and decomposes upon exposure to the high injector temperature during gas chromatography. Here we report the development of an improved method for detection of this compound by gas chromatography mass spectrometry (GCMS) using cold on-column injection of the sample. By using this sensitive method, it has been demonstrated that a lavandin (L. x intermedia) mutant accumulates trace quantities of linalool acetate and camphor and higher amounts of cineole and borneol compared to its parent. This plant, which very likely carries a point mutation in one or more of the genes involved in essential oil production, provides a unique tool for investigating regulation of essential oil biogenesis in plants.

  5. Antimicrobial susceptibility pattern of Brachyspira intermedia isolates from European layers.

    Science.gov (United States)

    Verlinden, Marc; Boyen, Filip; Pasmans, Frank; Garmyn, An; Haesebrouck, Freddy; Martel, An

    2011-09-01

    A broth microdilution method was used to determine the antimicrobial susceptibility of 20 Brachyspira intermedia isolates obtained from different layer flocks in Belgium and The Netherlands between 2008 and 2010. The antimicrobial agents used were tylosin, tilmicosin, tiamulin, valnemulin, doxycycline, and lincomycin. The minimal inhibitory concentration (MIC) distribution patterns of tylosin, tilmicosin, lincomycin, and doxycycline were bimodal, demonstrating acquired resistance against doxycycline in three strains, against the macrolides in two strains, and against lincomycin in one strain. The MICs of tiamulin and valnemulin showed a monomodal distribution, but with tailing toward the higher MIC values, possibly suggesting low-level acquired resistance in six isolates. Sequencing revealed a G1058C mutation in the 16S rRNA gene in all doxycycline-resistant strains. The strain resistant to tylosin, tilmicosin, and lincomycin had an A2058T mutation in the 23S rRNA gene.

  6. Approaches to management of beta-thalassemia intermedia

    Directory of Open Access Journals (Sweden)

    Joseph E. Maakaron

    2013-03-01

    Full Text Available Thalassemia intermedia is a genetically diverse group of diseases that is the result of an imbalance in the production of the alpha and beta chains with ensuing chronic hemolysis, ineffective erythropoiesis, and iron overload.Resulting complications include bone changes, hypercoagulability, and end-organ damage due to iron overload. This decade has witnessed major breakthroughs in the management of thalassemia. In this article, we examine these novelties in therapy including iron chelation therapy, stem cell transplant, and gene therapy.Iron chelation therapy has been revolutionized with the advent of deferasirox, a once-daily oral iron chelator, that has been shown to be safe and efficacious.Gene therapy was also at the core of this revolution with the discovery of novel gene elements and viral vectors allowing for better control and improved outcomes.

  7. Selección de bacterias con capacidad degradadora de hidrocarburos aisladas a partir de sedimentos del Caribe colombiano.

    OpenAIRE

    Narváez-Florez, S.; Gómez, M.L.; Martínez, M.M.

    2008-01-01

    A partir de sedimentos del Caribe colombiano se realizaron 31 aislamientos bacterianos en medio mínimo de sales suplementado con hidrocarburos (ACPM o petróleo crudo) como única fuente de carbono. Las cepas aisladas se sometieron a pruebas de selección en diferentes concentraciones de hidrocarburos y se escogieron once de ellas tolerantes al crudo y ACPM en un ámbito del 1-8% v/v. Posteriormente, con las cepas seleccionadas, se conformó un cultivo bacteriano mixto y se evaluó su capacidad de ...

  8. SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS AISLADAS A PARTIR DE SEDIMENTOS DEL CARIBE COLOMBIANO

    OpenAIRE

    Narváez-Flórez, Silvia; L. Gómez, Martha; Martínez, María M.

    2008-01-01

    A partir de sedimentos del Caribe colombiano se realizaron 31 aislamientos bacterianos en medio mínimo de sales suplementado con hidrocarburos (ACPM o petróleo crudo) como única fuente de carbono. Las cepas aisladas se sometieron a pruebas de selección en diferentes concentraciones de hidrocarburos y se escogieron once de ellas tolerantes al crudo y ACPM en un ámbito del 1-8% v/v. Posteriormente, con las cepas seleccionadas, se conformó un cultivo bacteriano mixto y se evaluó su capacidad de ...

  9. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells.

    Science.gov (United States)

    Tan, Dehong; Bai, Bing; Jiang, Donghua; Shi, Lin; Cheng, Shunchang; Tao, Dongbing; Ji, Shujuan

    2014-03-01

    The cytogenetic toxicity of rhodamine B on root tip cells of Allium cepa was investigated. A. cepa were cultured in water (negative control), 10 ppm methyl methanesulfonate (positive control), and three concentrations of rhodamine B (200, 100, and 50 ppm) for 7 days. Rhodamine B inhibited mitotic activity; increased nuclear anomalies, including micronuclei, nuclear buds, and bridged nuclei; and induced oxidative stress in A. cepa root tissues. Furthermore, a substantial amount of long nucleoplasmic bridges were entangled together, and some nuclei were simultaneously linked to several other nuclei and to nuclear buds with nucleoplasmic bridges in rhodamine B-treated cells. In conclusion, rhodamine B induced cytogenetic effects in A. cepa root tip cells, which suggests that the A. cepa root is an ideal model system for detecting cellular interactions.

  10. Splenectomy for people with thalassaemia major or intermedia.

    Science.gov (United States)

    Easow Mathew, Manu; Sharma, Akshay; Aravindakshan, Rajeev

    2016-06-14

    Thalassaemia is a genetic disease of the haemoglobin protein in red blood cells. It is classified into thalassaemia minor, intermedia and major, depending on the severity of the disease and the genetic defect. Thalassaemia major and intermedia require frequent blood transfusions to compensate for the lack of well-functioning red blood cells, although this need is significantly less in thalassaemia intermedia.Damaged or defective red blood cells are normally eliminated in the spleen. In people with thalassaemia there is a large quantity of defective red blood cells which results in an enlarged hyperfunctioning spleen (splenomegaly). Removal of the spleen may thus prolong red blood cell survival by reducing the amount of red blood cells removed from circulation and may ultimately result in the reduced need for blood transfusions. To assess the efficacy and safety of splenectomy in people with beta-thalassaemia major or intermedia. We searched the Cochrane Cystic Fibrosis and Genetic Disorders Review Group's Haemoglobinopathies Trials Register, compiled from searches of electronic databases and the handsearching of journals and conference abstract books. We also searched the reference lists of relevant articles and reviews.Date of the most recent search: 25 April 2016. We included randomised controlled studies and quasi-randomised controlled studies of people of any age with thalassaemia major or intermedia, evaluating splenectomy in comparison to conservative treatment (transfusion therapy and iron chelation) or other forms of splenectomy compared to each other (laparoscopic, open, radio-frequency). Two authors independently selected and extracted data from the single included study using a customised data extraction form and assessed the risk of bias. One study, including 28 participants was included in the review; the results were described, primarily, in a narrative manner. The study assessed the feasibility of splenectomy using laparoscopy in comparison to open

  11. Methylmercury as a mitosis disturbing agent. [Allium cepa; Drosophila melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    Ramel, C

    1969-01-01

    Experiments were performed to investigate the genetic effects of mercurials. These investigations included both cytological and genetical analyses. One of the main purposes of the investigations was to establish the lowest dose of the mercurials, which was genetically active. For the cytological work root tips cells of Allium cepa were used, while the genetical analyses were preformed on Drosophila melanogaster. The cytological tests on Allium included methyl mercury hydroxide, methyl mercury dicyandiamide, phenyl mercury hydroxide, and methoxyethylmercury chloride. The pesticide Panogen was also tested. The results from these studies are summarized.

  12. Development of Lutzomyia intermedia and Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae larvae in different diets

    Directory of Open Access Journals (Sweden)

    WERMELINGER E. D.

    2001-01-01

    Full Text Available The objective of this research was to evaluate, in laboratory, the development of Lutzomyia intermedia and Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae larvae, vectors of leishmaniasis in Brazil, in the following diets: industrialized food for rabbits, dogs, hamsters and aquarium fishes, besides liver powder, cooked lettuce, wheat germ, beer yeast, oat, wheat bran and a diet denominated aged food. Except wheat bran for L. intermedia, all diets provided adequate development for both species, which showed that any of them can be used in laboratory insectaries for these insects. L. intermedia showed better development with most nutritious diets and both species presented better development with aged food. Fungi as an additional nutrient source for L. intermedia and L. longipalpis is suggested.

  13. Draft genome sequence of a Kluyvera intermedia isolate from a patient with a pancreatic abscess.

    Science.gov (United States)

    Thele, Roland; Gumpert, Heidi; Christensen, Louise B; Worning, Peder; Schønning, Kristian; Westh, Henrik; Hansen, Thomas A

    2017-09-01

    The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain was reported as sensitive to Danish-registered antibiotics although it had a fosA-like gene in the genome. There were nine contigs that aligned to a plasmid, and these contigs contained several heavy metal resistance gene homologues. Furthermore, a prophage was discovered in the genome. WGS represents an efficient tool for monitoring Kluyvera spp. and its role as a reservoir of multidrug resistance. Therefore, this susceptible K. intermedia genome has many characteristics that allow comparison of resistant K. intermedia that might be discovered in the future. Copyright © 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

  14. In vitro Resistance Testing of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to Triclosan.

    Science.gov (United States)

    Farsi, Deema; Tanner, Anne

    2016-04-01

    To determine the sensitivity of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to triclosan, and determine if these bacteria develop resistance to triclosan upon prolonged exposure. Susceptibility to triclosan was tested against three periodontal pathogens P. gingivalis, P. intermedia, and T. forsythia. Escherichia coli strains sensitive and resistant to triclosan were used as biological controls to confirm the efficacy of triclosan in the assays. Agar plates were prepared locally with vitamin K and hemin-supplemented medium. Porphyromonas gingivalis and P. intermedia did not grow on plates containing ≥ 2 μg/ml triclosan, while T. forsythia did not grow on ≥ 1.66 μg/ml. Colonies of P. intermedia resistant to triclosan developed after prolonged incubation at 2 μg/ml, but this resistance disappeared during subculture in the absence of triclosan. No significant resistance to triclosan was detected for these species. Dental products containing triclosan can be beneficial in controlling periodontal disease.

  15. Patrón de los motivos EPIYA de cepas cubanas de Helicobacter pylori positivas para CagA

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    Lino E. Torres

    2012-03-01

    Conclusión. La mayoría de los aislamientos cubanos presentaron las combinaciones de motivos EPIYA menos virulentas (ABC. Los resultados del empleo de los nuevos cebadores y el análisis de la secuenciación, confirmaron que todas las cepas estudiadas portaban el gen cagA de tipo occidental. Ninguno de los patrones específicos de EPIYA se asoció con úlcera péptica. Este es el primer reporte que muestra la distribución de los motivos EPIYA en los aislamientos de H. pylori de la región del Caribe.   DOI: http://dx.doi.org/10.7705/biomedica.v32i1.453

  16. Protective effect of an egg yolk-derived immunoglobulin (IgY) against Prevotella intermedia-mediated gingivitis.

    Science.gov (United States)

    Hou, Y-Y; Zhen, Y-H; Wang, D; Zhu, J; Sun, D-X; Liu, X-T; Wang, H-X; Liu, Y; Long, Y-Y; Shu, X-H

    2014-04-01

    To investigate the effects of an egg yolk-derived immunoglobulin (IgY) specific to Prevotella intermedia in vitro and in vivo. An IgY specific to P. intermedia was produced by immunizing hens with formaldehyde-inactivated P. intermedia and showed high titres when subjected to an ELISA. The obtained IgY inhibited the growth of P. intermedia in a dose-dependent manner at concentrations from 1 to 20 mg ml(-1) in Center for Disease Control and Prevention liquid medium. Forty rats were challenged with P. intermedia on gingivae and then randomly divided into four groups, which were syringed respectively with phosphate-buffered saline, 1 mg ml(-1) of tinidazole, 20 mg ml(-1) of nonspecific IgY and 20 mg ml(-1) of the IgY specific to P. intermedia at a dosage of 300 μl per day. Gingival index (GI), plaque index (PI), bleeding on probing (BOP), counts of white blood cell (WBC) and histopathological slide of the gums were measured after treatment for 15 days. The gingivitis rats treated with the IgY specific to P. intermedia showed significantly decreased GI, PI, BOP and WBC (P intermedia-mediated gingivitis. A new immunoglobulin specific to P. intermedia was developed from egg yolk. This specific IgY can dose-dependently inhibit the growth of P. intermedia and protect rats from gingivitis induced by P. intermedia. The new IgY has potential for the treatment of P. intermedia-mediated gingivitis. © 2013 The Society for Applied Microbiology.

  17. Estudios de laboratorio para el control de Ceratitis capitata (Wiedmann (Diptera: Tephritidae (Mosca del Mediterráneo con Beauveria bassiana

    Directory of Open Access Journals (Sweden)

    Luis Porras

    2008-01-01

    Full Text Available Con el objetivo de seleccionar cepas promisorias de Beauveria bassiana para el control de Ceratitis capitata, se realizó, en condiciones de laboratorio, una serie de ensayos empleando la mosca adulta como estado vulnerable al parasitismo por el hongo entomopatógeno. Para la selección se utilizó una concentración de 5x109 CV.m-2 del hongo. Se determinó la CL90, la sobrevivencia media (SM, la compatibilidad con insecticidas, además de ensayos de producción con el hongo. Así como la compatibilidad de los insecticidas Mercaptotion 100% CE (Lupara® y Dimetoato 40% CE (Sistémico Icona con las cepas seleccionadas. Se cuantificó la viabilidad de los conidios en platos de Petri pos-tratamiento, a 50, 100 y 200% de la dosis comercial del insecticida. A partir de 7 cepas evaluadas (mortalidad 97-14% fueron seleccionadas la Bb 26, la Bb 259, la Bb 132, y la Bb 238. Los valores de CL90 estuvieron entre 1x109 y 3,8 x1011 CV.m-2 (Bb 238 y Bb 26, respectivamente. La SM de las cepas varió de 7-12 días (Bb 238 y Bb 26, respectivamente. La mayor producción fue de 7x109 UFC.mg-1 y 20,7 g.conidios.kg-1 de arroz (Bb 26. Todas las cepas fueron compatibles con Mercaptotion y la Bb 132 presentó la mayor compatibilidad con el Dimetoato. En conclusión, los ensayos en el laboratorio permitieron seleccionar cepas de B. bassiana con potencial para controlar C. capitata.

  18. Bacterias ácido lácticas con capacidad antagónica y actividad bacteriocinogénica aisladas de quesos frescos

    Directory of Open Access Journals (Sweden)

    Cástulo I. Martín del Campo M.

    2008-01-01

    Full Text Available Se aislaron 350 cepas de bacterias ácido lácticas (BAL, a partir de 35 muestras de quesos frescos, fueron probadas en contra de cuatro microorganismos patógenos, tres Gram+ (Listeria monocytogenes, Staphylococcus aureus, Streptococcus pyogenes y un Gram- (Salmonella agona. Sólo 25 cepas mostraron capacidad antagónica, el mayor efecto inhibidor fue debido al pH, por la producción de ácidos orgánicos. 8 de ellas, mostraron un efecto inhibidor diferente al pH, Todas las cepas mostraron actividad antagónica en contra de bacterias Gram+. S. agona, no fue inhibida en su desarrollo, por ninguna de las cepas de bacterias ácido lácticas. Tres cepas que mostraron inhibición con el sobrenadante, se trataron con enzimas proteolíticas, y se determinó que el factor inhibidor es de origen proteico. 16 cepas mostraron que es necesaria la presencia de las BAL para inhibir a los patógenos, al probar el sobrenadante libre de BAL, el efecto inhibidor no se manifestó.

  19. In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm.

    Science.gov (United States)

    Jang, Eun-Young; Kim, Minjung; Noh, Mi Hee; Moon, Ji-Hoi; Lee, Jin-Yong

    2016-02-01

    Polyphosphate (polyP) has gained a wide interest in the food industry due to its potential as a decontaminating agent. In this study, we examined the effect of sodium tripolyphosphate (polyP3; Na5P3O10) against planktonic and biofilm cells of Prevotella intermedia, a major oral pathogen. The MIC of polyP3 against P. intermedia ATCC 49046 determined by agar dilution method was 0.075%, while 0.05% polyP3 was bactericidal against P. intermedia in time-kill analysis performed using liquid medium. A crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that sub-MICs of polyP3 significantly decreased biofilm formation. Under the scanning electron microscope, decreased numbers of P. intermedia cells forming the biofilms were observed when the bacterial cells were incubated with 0.025% or higher concentrations of polyP3. Assessment of biofilm viability with LIVE/DEAD staining and viable cell count methods showed that 0.05% or higher concentrations of polyP3 significantly decreased the viability of the preformed biofilms in a concentration-dependent manner. The zone sizes of alpha-hemolysis formed on horse blood agar produced by P. intermedia were decreased in the presence of polyP3. The expression of the genes encoding hemolysins and the genes of the hemin uptake (hmu) locus was downregulated by polyP3. Collectively, our results show that polyP is an effective antimicrobial agent against P. intermedia in biofilms as well as planktonic phase, interfering with the process of hemin acquisition by the bacterium. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  20. Incidence of Prevotella intermedia and Prevotella nigrescens Carriage among Family Members with Subclinical Periodontal Disease

    Science.gov (United States)

    Fukui, Katsuhito; Kato, Naoki; Kato, Haru; Watanabe, Kunitomo; Tatematsu, Norichika

    1999-01-01

    We established a typing system for Prevotella intermedia and Prevotella nigrescens using the combination of PCR ribotyping and arbitrarily primed PCR (AP-PCR) fingerprinting and applied this system to the study of intrafamilial incidence of these species in the oral cavity. PCR ribotyping followed by subtyping by AP-PCR fingerprinting was applied to each type strain of P. intermedia and P. nigrescens and 54 isolates (32 isolates of P. intermedia and 24 isolates of P. nigrescens) from extraoral infections, resulting in an excellent discriminatory power (discrimination index, 0.99) for both species. A total of 18 subjects from six families, with the subjects from each family comprising the mother, the father, and a child who had subclinical early-stage to moderate adult periodontitis or simple gingivitis and who carried P. intermedia or P. nigrescens, or both, were enrolled in the study of intrafamilial carriage. When 20 colonies per specimen of subgingival plaque, if available, were picked from primary culture, 115 P. intermedia and 178 P. nigrescens isolates were recovered from the 18 subjects. Among the subjects studied, family members shared the same subtype strain(s) but non-family members did not. Multiple subtypes were found in 8 (57%) of the 14 P. nigrescens-positive subjects but in only 3 (27%) of the 11 P. intermedia-positive subjects; the difference was, however, not statistically significant (P = 0.14). These results suggest that the combination of PCR ribotyping and AP-PCR fingerprinting is well suited for the epidemiological study of P. intermedia and P. nigrescens and that each family seems to carry a distinct subtype(s) of these species. PMID:10488167

  1. Characterization of Atypical Isolates of Yersinia intermedia and Definition of Two New Biotypes▿ †

    Science.gov (United States)

    Martin, Liliane; Leclercq, Alexandre; Savin, Cyril; Carniel, Elisabeth

    2009-01-01

    The species Yersinia intermedia is a member of the genus Yersinia which belongs to the Enterobacteriaceae family. This species is divided into eight biotypes, according to Brenner's biotyping scheme. This scheme relies on five tests (utilization of Simmons citrate and acid production from d-melibiose, d-raffinose, α-methyl-d-glucoside [αMG], and l-rhamnose). The collection of the French Yersinia Reference Laboratory (Institut Pasteur, Paris, France) contained 44 strains that were originally identified as Y. intermedia but whose characteristics did not fit into the biotyping scheme. These 44 strains were separated into two biochemical groups: variant 1 (positive for acid production from l-rhamnose and αMG and positive for Simmons citrate utlization) and variant 2 (positive for acid production from l-rhamnose and αMG). These atypical strains could correspond to new biotypes of Y. intermedia, to Y. frederiksenii strains having the atypical property of fermenting αMG, or to new Yersinia species. These strains did not exhibit growth or phenotypic properties different from those of Y. intermedia and Y. frederiksenii and did not harbor any of the virulence traits usually found in pathogenic species. DNA-DNA hybridizations performed between one strain each of variants 1 and 2 and the Y. intermedia and Y. frederiksenii type strains demonstrated that these variants do belong to the Y. intermedia species. We thus propose that Brenner's biotyping scheme be updated by adding two new biotypes: 9 (for variant 1) and 10 (for variant 2) to the species Y. intermedia. PMID:19494062

  2. Quorum sensing signal profile of Acinetobacter strains from nosocomial and environmental sources Perfil de sensores de quórum en cepas nosocomiales y ambientales de Acinetobacter

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    R. H. González

    2009-06-01

    Full Text Available A set of 43 strains corresponding to 20 classified and unclassified genomic Acinetobacter species was analyzed for the production of typical N-acyl homoserine lactone quorum sensing molecules in culture broths. A large percentage of the strains (74% displayed quorum sensing signals that could be separated into three statistically significantly different chromatographic groups (p Rf2 > Rf1. None of the three signals could be specifically assigned to a particular species in the genus; furthermore, no distinction could be made between the quorum sensing signals secreted by typical opportunistic strains of the A. calcoaceticus-A. baumannii complex, isolated from patients, with respect to the other species of the genus, except for the Rf1 signal which was present in all the QS positive strains belonging to this complex and DNA group 13 TU. In conclusion, quorum sensors in Acinetobacter are not homogenously distributed among species and one of them is present in most of the A. calcoaceticus-baumannii complex.Se analizó la producción de moléculas típicas de N-acil homoserina lactona con actividad de quorum sensing en cultivos líquidos de un grupo de 43 cepas correspondientes a 20 especies genómicas clasificadas y no clasificadas de Acinetobacter. Un porcentaje alto de las cepas (74% mostraron señales de quorum sensing que pudieron ser separadas en tres grupos cromatográficos significativamente diferentes entre sí (p Rf2 > Rf1. Ninguna de las tres señales pudo ser asignada a una especie en particular dentro del género; es más, no se encontró diferencia entre las señales producidas por las cepas típicamente oportunistas (complejo A. calcoaceticus-A. baumannii aisladas de pacientes respecto de las producidas por otras cepas del mismo género, excepto para el caso de Rf1, que se encontró presente en todos los aislamientos quorum sensing positivos del mencionado complejo y en las cepas del grupo de DNA 13TU. En conclusión, los sensores de

  3. Gravitropism in caulonemata of the moss Pottia intermedia

    Science.gov (United States)

    Chaban, C. I.; Kern, V. D.; Ripetskyj, R. T.; Demkiv, O. T.; Sack, F. D.

    1998-01-01

    The gravitropism of caulonemata of Pottia intermedia is described and compared with that of other mosses. Spore germination produces primary protonemata including caulonemata which give rise to buds that form the leafy moss plant, the gametophore. Primary caulonemata are negatively gravitropic but their growth and the number of filaments are limited in the dark. Axenic culture of gametophores results in the production of secondary caulonemata that usually arise near the leaf base. Secondary protonemata that form in the light are agravitropic. Secondary caulonemata that form when gametophores are placed in the dark for several days show strong negative gravitropism and grow well in the dark. When upright caulonemata are reorientated to the horizontal or are inverted, upward bending can be detected after 1 h and caulonemata reach the vertical within 1-2 d. Clear amyloplast sedimentation occurs 10-15 minutes after horizontal placement and before the start of upward curvature. This sedimentation takes place in a sub-apical zone. Amyloplast sedimentation also takes place along the length of upright and inverted Pottia protonemata. These results support the hypothesis that amyloplast sedimentation functions in gravitropic sensing since sedimentation occurs before gravitropism in Pottia and since the location and presence of a unique sedimentation zone is conserved in all four mosses known to gravitropic protonomata.

  4. DETERMINACIÓN DE MEDIOS DE CULTIVO Y PH PARA LA MASIFICACIÓN in vitro DE CEPAS DE Suillus luteus Aubl. ASOCIADAS A Pinus radiata D. Don Y Scleroderma citrinum Pers. ASOCIADAS A Eucalyptus globulus Labill. DE LA REGIÓN DEL BIOBÍO, CHILE

    Directory of Open Access Journals (Sweden)

    Marta González

    2015-02-01

    Full Text Available El uso de agentes biológicos en la producción en vivero ha aflorado como un elemento que permite no sólo mejorar la calidad morfológica y fisiológica de las plantas, sino que también posibilitar un mayor éxito en el ámbito silvicultural de una plantación. Estos agentes biológicos requieren de un ambiente óptimo para crecer, asociarse y reproducirse. El objetivo de este estudio fue determinar la dependencia del pH y el medio de cultivo en el crecimiento in vitro de distintas cepas de Suillus luteus y Scleroderma citrinum asociadas a Pinus radiata y Eucalyptus globulus, respectivamente. El estudio se realizó en condiciones controladas de temperatura y humedad, disponiendo los inóculos en placas Petri con diferentes medios cultivo, evaluando parámetros de crecimiento y biomasa a los 38 días para S. luteus y 105 días para S. citrinum. Los resultados indican que tanto el medio de cultivo, el pH del medio, así como las cepas de cada especie estudiada, son determinantes en las respuestas de crecimiento de los hongos ectomicorrícicos in vitro evaluados. Las cepas de S. luteus se desarrollaron adecuadamente en un medio de cultivo con abundancia de nutrientes (BAF, MMN como en baja presencia de ellos (EMA, y con pH más bien ácido (4,8 y 5,8. Por otro lado, las cepas de S. citrinum presentaron, para los mismos ambientes, un desarrollo inferior y lento, no obstante, la cepa Sc8 se reprodujo de forma óptima y rápida bajo un medio de cultivo BAF y con un pH moderadamente ácido de 5,8.

  5. Effect of estradiol on planktonic growth, coaggregation, and biofilm formation of the Prevotella intermedia group bacteria.

    Science.gov (United States)

    Fteita, Dareen; Könönen, Eija; Söderling, Eva; Gürsoy, Ulvi Kahraman

    2014-06-01

    Alterations in the quantity and quality of biofilms at gingival margin are considered to play a role in the initiation and development of pregnancy-related gingivitis. Prevotella intermedia sensu lato is able to consume estradiol, the major sex hormone secreted during pregnancy, in the absence of vitamin K. The aim of the study was to examine the effect of estradiol on the planktonic growth, coaggregation, polysaccharide production, and biofilm formation of the P. intermedia group bacteria, namely P. intermedia, Prevotella nigrescens, and Prevotella pallens. In all experiments, the type strain (ATCC) and a clinical strain (AHN) of P. intermedia, P. nigrescens, and P. pallens were incubated with the concentrations of 0, 30, 90, and 120 nmol/L of estradiol. Planktonic growth was assessed by means of the colony forming unit method, while coaggregation and biofilm formation were assessed by spectrophotometric methods. In the determination of protein and polysaccharide levels, the Bradford and phenol-sulfuric acid methods were used, respectively. P. pallens AHN 9283 and P. nigrescens ATCC 33563 increased their numbers at planktonic stage with increasing estradiol concentrations. In 48-h biofilm tests, elevated protein levels were found for both strains of P. intermedia, and the strains P. nigrescens ATCC 33563 and P. pallens AHN 9283 in the presence of estradiol. The P. intermedia strains also increased the levels of polysaccharide formation in the biofilm. Coaggregation of the P. intermedia group organisms with Fusobacterium nucleatum was enhanced only in P. intermedia AHN 8290. In conclusion, our in vitro experiments indicate that estradiol regulates planktonic growth, coaggregation, polysaccharide production, and biofilm formation characteristics of P. intermedia, P. nigrescens, and P. pallens differently. These results may, at least partly, explain the differences seen in their contribution to the pathogenesis of pregnancy-related gingivitis

  6. Tropical House Gecko (Hemidactylus mabouia predation on brown spiders (Loxosceles intermedia

    Directory of Open Access Journals (Sweden)

    E.N. Ramires

    2004-01-01

    Full Text Available Brown spiders (Loxosceles spp. are venomous arachnids, successfully adapted to urban habitats in Brazil. Loxoscelism became a serious public health problem in Paraná State, especially at the capital Curitiba, where the most abundant species is Loxosceles intermedia. Hemidactylus mabouia (Gekkonidae lizards are synanthropic predators of arthropods. In this paper, we describe the predatory behavior of the Tropical House Gecko H. mabouia on L. intermedia under laboratory conditions. Twelve geckos were observed, and all of them fed on brown spiders (n=123 observations. The attack consisted of a fast run followed by one bite on the spider’s abdomen or legs. The geckos did not attack L. intermedia anterior body parts, probably due to the fangs present in this region. Two Hemidactylus individuals were killed by L. intermedia bites: during a predatory encounter, and by an induced bite on a restrained lizard. The observations summarized in this paper show that H. mabouia could be used in the biological control of Loxosceles populations in human dwellings. However, additional field studies are necessary to quantify the impact of H. mabouia predation on urban populations of L. intermedia and other species of the same genus.

  7. Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia.

    Science.gov (United States)

    Johnston, Christopher D; Skeete, Chelsey A; Fomenkov, Alexey; Roberts, Richard J; Rittling, Susan R

    2017-01-01

    Prevotella intermedia, a major periodontal pathogen, is increasingly implicated in human respiratory tract and cystic fibrosis lung infections. Nevertheless, the specific mechanisms employed by this pathogen remain only partially characterized and poorly understood, largely due to its total lack of genetic accessibility. Here, using Single Molecule, Real-Time (SMRT) genome and methylome sequencing, bisulfite sequencing, in addition to cloning and restriction analysis, we define the specific genetic barriers to exogenous DNA present in two of the most widespread laboratory strains, P. intermedia ATCC 25611 and P. intermedia Strain 17. We identified and characterized multiple restriction-modification (R-M) systems, some of which are considerably divergent between the two strains. We propose that these R-M systems are the root cause of the P. intermedia transformation barrier. Additionally, we note the presence of conserved Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) systems in both strains, which could provide a further barrier to exogenous DNA uptake and incorporation. This work will provide a valuable resource during the development of a genetic system for P. intermedia, which will be required for fundamental investigation of this organism's physiology, metabolism, and pathogenesis in human disease.

  8. Acoustic signals in the sand fly Lutzomyia (Nyssomyia intermedia (Diptera: Psychodidae

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    Peixoto Alexandre A

    2011-05-01

    Full Text Available Abstract Background Acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. Here we report the courtship songs of the sand fly Lutzomyia (Nyssomyia intermedia, one of the main vectors of cutaneous leishmaniasis in Brazil. Findings Recordings were performed using insects from three localities from Eastern Brazil: Posse and Jacarepaguá in Rio de Janeiro State and Corte de Pedra in Bahia State. The three areas have remnants of the Brazilian Atlantic forest, they are endemic for cutaneous leishmaniasis and L. intermedia is the predominant sand fly species. We observed that during courtship L. intermedia males from all populations produced pulse songs consisting of short trains. No significant differences in song parameters were observed between the males of the three localities. Conclusions L. intermedia males produce acoustic signals as reported for some other sand flies such as the sibling species of the Lutzomyia longipalpis complex. The lack of differences between the males from the three localities is consistent with previous molecular studies of the period gene carried out in the same populations, reinforcing the idea that L. intermedia is not a species complex in the studied areas and that the three populations are likely to have similar vectorial capacities.

  9. Acoustic signals in the sand fly Lutzomyia (Nyssomyia) intermedia (Diptera: Psychodidae)

    Science.gov (United States)

    2011-01-01

    Background Acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. Here we report the courtship songs of the sand fly Lutzomyia (Nyssomyia) intermedia, one of the main vectors of cutaneous leishmaniasis in Brazil. Findings Recordings were performed using insects from three localities from Eastern Brazil: Posse and Jacarepaguá in Rio de Janeiro State and Corte de Pedra in Bahia State. The three areas have remnants of the Brazilian Atlantic forest, they are endemic for cutaneous leishmaniasis and L. intermedia is the predominant sand fly species. We observed that during courtship L. intermedia males from all populations produced pulse songs consisting of short trains. No significant differences in song parameters were observed between the males of the three localities. Conclusions L. intermedia males produce acoustic signals as reported for some other sand flies such as the sibling species of the Lutzomyia longipalpis complex. The lack of differences between the males from the three localities is consistent with previous molecular studies of the period gene carried out in the same populations, reinforcing the idea that L. intermedia is not a species complex in the studied areas and that the three populations are likely to have similar vectorial capacities. PMID:21569534

  10. Acoustic signals in the sand fly Lutzomyia (Nyssomyia) intermedia (Diptera: Psychodidae).

    Science.gov (United States)

    Vigoder, Felipe M; Souza, Nataly A; Peixoto, Alexandre A

    2011-05-13

    Acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. Here we report the courtship songs of the sand fly Lutzomyia (Nyssomyia) intermedia, one of the main vectors of cutaneous leishmaniasis in Brazil. Recordings were performed using insects from three localities from Eastern Brazil: Posse and Jacarepaguá in Rio de Janeiro State and Corte de Pedra in Bahia State. The three areas have remnants of the Brazilian Atlantic forest, they are endemic for cutaneous leishmaniasis and L. intermedia is the predominant sand fly species. We observed that during courtship L. intermedia males from all populations produced pulse songs consisting of short trains. No significant differences in song parameters were observed between the males of the three localities. L. intermedia males produce acoustic signals as reported for some other sand flies such as the sibling species of the Lutzomyia longipalpis complex. The lack of differences between the males from the three localities is consistent with previous molecular studies of the period gene carried out in the same populations, reinforcing the idea that L. intermedia is not a species complex in the studied areas and that the three populations are likely to have similar vectorial capacities.

  11. Effect of Aqueous Allium Cepa Extract from Red Onion on Aluminum ...

    African Journals Online (AJOL)

    ADOWIE PERE

    2017-12-14

    Dec 14, 2017 ... ABSTRACT: This study examined the protective effect of Allium cepa extract from red onion on aluminum – ... permits unrestricted use, distribution, and reproduction in any .... water, air dried, and 200 g was then blended. The.

  12. CÓMO SE NEGOCIA UNA POSICIÓN DE SUJETO INTERMEDIA: MUCHACHOS ENTRE ONCE Y CATORCE AÑOS, MASCULINIDADES Y EDUCACIÓN ESCOLAR

    Directory of Open Access Journals (Sweden)

    Ann Phoenix

    2002-01-01

    Full Text Available Este ensayo pone a discusión la ciencia (generalizada en las escuelas de Londres según la cual existe una oposición entre la masculinidad y la realización de tareas escolares, que tiene fuertes efectos en la subjetividad de lo masculino del joven. Así considera las formas en que éstos asisten a las escuelas de Londres y negocian lo que ellos consideran las contradicciones que involucra el tratar de reconciliar el hacer las tareas escolares con ser aceptado por los otros en su condición masculina. El documento se centra particularmente en la forma como los muchachos negocian una posición de sujeto intermedia, en un intento de asegurarse que trabajaron lo suficiente para no reprobar, pero no tanto como para llamar la atención de los otros, por no ser "propiamente masculinos". El texto también afirma que estas contradicciones tienen una carga emocional, de modo que los jóvenes se esfuerzan en negociar lo que ellos consideran una posición intermedia, pues esta es la posición de sujeto que les resulta menos problemática ocupar.

  13. Evolución de estrellas enanas blancas de Helio de masa baja e intermedia

    Science.gov (United States)

    Althaus, L. G.; Benvenuto, O. G.

    Numerosas observaciones realizadas particularmente en los últimos dos años parecen confirmar que las enanas blancas (EB) de helio de masa baja e intermedia son el resultado de la evolución de sistemas binarios cercanos. Con el objeto de realizar una adecuada interpretación de estos objetos son necesarios modelos de EBs de helio lo más detallado posibles. En este estudio presentamos cálculos detallados de la evolución de EBs de helio con masas entre M=0.1Msolar y M=0.5Msolar a intervalos de 0.05Msolar . Para ello, hemos tenido en cuenta los efectos de temperatura finita mediante un código de evolución estelar lo más actualizado posible. En particular, el transporte de energía es descripto en el marco del nuevo modelo para la convección turbulenta desarrollado por Canuto - Mazzitelli. Además hemos considerado la nueva ecuación de estado para plasmas de helio de Saumon et al. y nuevas opacidades radiativas OPAL. Las pérdidas por neutrinos fueron asimismo tenidas en cuenta. Excepto para las EBs más masivas, nuestros modelos iniciales están ubicados en las cercanías de la correspondiente línea de Hayashi para configuraciones de helio. Nuestros resultados muestran que existe una región prohibida en el diagrama observacional HR donde ninguna EB de helio puede encontrarse. Dicha región es para log{(L/Lsolar )}>= -0.25 and log{Teff} >= 4.45. Hemos encontrado también que los tracks evolutivos en el diagrama HR en el dominio de alta luminosidad (pre - EB) son fuertemente afectados por la eficiencia convectiva y que las pérdidas por neutrinos son importantes en los modelos más masivos. Finalmente hemos analizado la estructura de la zona convectiva externa encontrando que la teoría de Canuto - Mazzitelli conduce a un perfil convectivo muy diferente del dado por cualquier versión de la popular teoría de la mixing length. Si bién este comportamiento es decisivo en el contexto de las inestabilides pulsacionales, los radios y gravedades superficiales de

  14. Estrategia de verificación de calidad de las cepas de Escherichia coli conservadas en la Colección del Centro de Ingeniería Genética y Biotecnología

    OpenAIRE

    Iveldris Domínguez-Vázquez; Evelyn Olano-Ruiz; Angela Estela Sosa-Espinosa

    2010-01-01

    La colección de microorganismos de interés biotecnológico del Centro de Ingeniería Genética y Biotecnología incluye diferentes cepas de bacterias y levaduras utilizables en la manipulación de genes mediante el empleo de la ingeniería genética y para la producción de proteínas recombinantes por métodos biotecnológicos. La colección de hospederos de Escherichia coli centraliza todas las cepas que se utilizan en los proyectos de investigación-desarrollo de la institución. Este microorganismo con...

  15. Fagotipagem de cepas de Staphylococcus aureus resistentes a antibióticos, isoladas de leite

    Directory of Open Access Journals (Sweden)

    Wanderley Pereira de Araújo

    1998-01-01

    Full Text Available A partir de amostras de leite cru foram isoladas 201 cepas de S. aureus, as quais foram submetidas a provas de resistência a antibióticos pelo método dos discos impregnados com os seguintes antibacterianos: amicacina, ampicilina, cefalotina, cefoxitina, cloranfenicol, clindamicina, oxacilina, penicilina, tetraciclina, tobramicina e vancomicina. Com exceção de 88 (43,8%, 90 (44,8%, 24 (11,9% e 40 (19,9% cepas resistentes à penicilina, ampicilina, cloranfenicol e tetraciclina, respectivamente, a maioria das 201 cepas (95% ou mais foi sensível aos antibióticos utilizados. As cepas resistentes a estes quatro antibióticos foram fagotipadas, empregando-se os Conjuntos Básicos Internacionais de bacteriófagos para cepas de origem humana e bovina, verificando-se que houve predomínio de cepas sensíveis a fagos dos grupos III e III/NC do conjunto básico humano e a fagos dos grupos III, IV e da associação III/IV do conjunto bovino.

  16. Prevotella intermedia and Porphyromonas gingivalis in dental caries with periapical granuloma

    Directory of Open Access Journals (Sweden)

    Risya Cilmiaty

    2013-12-01

    Full Text Available Background: Dental caries with necrotic pulp is a multifactorial disease that attacks enamel involving tooth pulp. The anaerobic bacteria infection in the pulp chamber could induce the formation of periapical granuloma. However, the presence of the most frequently anaerobic bacteria identified in apical periodontitis, Porphyromonas gingivalis and Prevotella intermedia, in periapical granuloma have not been confirmed. Purpose: The aims of study were to determine the presence of Porphyromonas gingivalis and Prevotella intermedia in dental caries with necrotic pulp and to determine its relation to periapical granuloma. Methods: Thirty-six patients of dental caries with necrotic pulp in Dr. Moewardi General Hospital in Surakarta, Indonesia were involved and classified into two groups, the group of patients with periapical granuloma and the group of patients without periapical granuloma. The caries tooth was extracted, and the chronic periapical tissue was swabbed and cultured on blood agar medium in anaerobic condition. The bacterial DNA was extracted from the positive cultures and subjected for Polymerase Chain Reaction (PCR. Results: Periapical granuloma was more likely found in women (OR 5.5, 95% CI=1.277-23.693; RR 2.5, 95% CI= 1.025-6.100. Black colonies bacteria were associated with periapical granuloma (OR 2.2, 95% CI=0.517-9.594; RR 1.5, 95% CI=0.655-3.623. Porphyromonas gingivalis and Prevotella intermedia were detected in group with or without periapical granuloma, however, only Prevotella intermedia was associated with periapical granuloma (OR 1.6, 95% CI=0.418-5.903; RR 1.3, 95% CI=0.653-2.393. Conclusion: The presence of Porphyromonas gingivalis and Prevotella intermedia in periapical granuloma were confirmed, however, only Prevotella intermedia were associated with periapical granuloma.Latar belakang: Karies gigi dengan pulpa nekrosis adalah penyakit multifaktorial yang menyerang enamel hingga ruang pulpa gigi. Infeksi bakteri anaerob

  17. Digital Distribution of Advertising for Publications (DDAP): a graphic arts prototype of electronic intermedia publishing (EIP)

    Science.gov (United States)

    Dunn, Patrice M.

    1998-01-01

    The Digital Distribution of Advertising for Publications (DDAP) is a graphic arts industry prototype of Electronic Intermedia Publishing (EIP). EIP is a strategic, multi- industrial concept that seeks to enable the capture and input of volumes of data (i.e., both raster and object oriented data -- as well as the latter's antecedent which is vector data -- color data and black-and-white data) from a multiplicity of devices; then flowing, controlling, manipulating, modifying, storing, retrieving, transmitting, and shipping, that data through an industrial process for output to a multiplicity of output devices (e.g., ink on paper, toner on paper, bits and bytes on CD ROM, Internet, Multimedia, HDTV, etc.). As the technical requirements of the print medium are among the most rigorous in the Intermedia milieu the DDAP prototype addresses some of the most challenging issues faced in Electronic Intermedia Publishing (EIP).

  18. Identificación y Caracterización de Cepas Nativas de Rhizobium en la Provincia de Santa Elena.

    Directory of Open Access Journals (Sweden)

    Javier Soto

    2015-10-01

    Full Text Available En los sistemas agrícolas de subsistencia, el nitrógeno es un macronutriente esencial para el buen desarrollo de las plantas; es utilizado en forma de nitrato o amonio. Las bacterias del género Rhizobium poseen la capacidad de fijar el nitrógeno, mediante una relación de simbiosis con leguminosas e influyen en la producción de sustancias promotoras de crecimiento en las plantas no leguminosas. En la península de Santa Elena no existen evidencias del uso y aplicación de biofertilizantes de origen microbiano nativo, por lo que el presente trabajo tuvo como objetivo caracterizar y seleccionar cepas del género Rhizobium procedentes de los nódulos de leguminosas de dos zonas de la provincia de Santa Elena. Los experimentos Aislamiento, identificación y caracterización bioquímica de cepas de Rhizobium encontradas en diferentes zonas de la península de Santa Elena comprende: a Estudio de la morfología nodular de leguminosas; b Presencia de la enzima Leghemoglobina en los nódulos, c Aislamiento de microorganismos de los nódulos de leguminosas, d Respuesta de la enzima catalasa ante el peróxido de hidrógeno, e Crecimiento en diferentes fuentes de carbono , f Crecimiento en tres porcentajes cloruro de sodio; y e Producción de 3-ketolactasa en agar LLA para descartar contaminantes frecuentes como Agrobacterium.

  19. Serum Prohepcidin Levels in Children with Thalassemia Major and Intermedia

    Directory of Open Access Journals (Sweden)

    Celil Yılmaz

    2017-04-01

    Full Text Available Objective: To determine the role of hepcidin hormone levels in iron accumulation in patients with thalassemia major (TM and thalassemia intermedia (TI. Materials and Methods: Serum prohepcidin and ferritin levels were determined in 34 patient with TM, 10 patient with TI, who attended the Department of Pediatric Hematology Adnan Menderes University Medical Faculty and the Department of Pediatrics at Aydın Atatürk State Hospital between 1 September 2006 and 30 September 2007 and 40 control patients without infection/inflammation, hepatitis or liver failure. Serum prohepcidin levels were measured using a commercial enzyme-linked immunosorbent assay kit (DRG International, Inc. Marburg, Germany; ferritin was studied with chemiluminescence method (Immulite 2000 DPC. Results: Mean serum ferritin levels in TM, TI and control groups were 2347.97±1724.81 ng/mL (range: 144-8015 ng/mL, 1352.40±918.94 ng/mL (range: 311-3109 ng/mL, and 33.35±12.03 ng/mL (range: 20-69.1 ng/mL, respectively. Serum prohepcidin levels in the same groups were 221.78±74.38 ng/mL (range: 7l.14-446.57 ng/mL, 173.31±52.14 ng/mL (range: 100.83-267.69 ng/mL, and 218.20±50.37 ng/mL (range: 116.18-330.43 ng/mL, respectively. There was a statistically significant difference in prohepcidin levels between patients with TI and control group only (p=0.016. No correlation was found between prohepcidin and ferritin levels in all groups (r=-0.023, p=0.839. Conclusion: Low levels of prohepcidin in patients with TI may be related to increased erythropoietic activity. Prohepcidin can be an indicator of active erythropoiesis.

  20. Evaluación de la producción de cuerpos fructíferos de cepas guatemaltecas del hongo comestible Rukoxil Tunay Che’ (Agrocybe cylindracea (DC.: Fr. Maire en diferentes sustratosEvaluation of the production of fruiting bodies of the

    Directory of Open Access Journals (Sweden)

    María del Carmen Bran

    2014-10-01

    Full Text Available En el presente estudio se determinó la producción de cuerpos fructíferos de cinco cepas nativas de Agrocybe cylindracea sobre tres sustratos y dos tratamientos térmicos, a través del porcentaje de eficiencia biológica y la medición del diámetro de los píleos. Se encontró que el mayor porcentaje de eficiencia biológica de las cepas en los sustratos evaluados fue 115.84 %, que correspondió al sustrato constituido por 29% paja de trigo mas 1% de harina de soya pasteurizado y obtenido por la cepa 58.01, la cual también produjo las mayores eficiencias biológicas en todos los sustratos evaluados. Al confrontar el porcentaje de eficiencia biológica en los diferentes sustratos, todas las cepas presentaron valores altos en el sustrato compuesto por 28% de paja de trigo, 1% harina de soya y CaCO3. Con respecto al diámetro de los cuerpos fructíferos, las cepas 58.01, 59.01, 60.01 y 638.08 produjeron píleos menores de 2 cm, entre 2-4 cm y mayores a 4.0 cm en los diferentes sustratos y tratamientos, excepto la cepa 59.01 que en el sustrato formulado con 29% de paja de trigo y 1% de harina de soya, solo produjo cuerpos fructíferos con píleos menores a 2 cm y entre 2-4 cm. En el análisis proximal de los basidiomas de las cepas evaluadas se obtuvo un alto porcentaje de proteínas, fibra cruda y carbohidratos, así como bajo porcentaje de grasas. Se recomienda que en futuras investigaciones o transferencia de tecnología a comunidades o entidades interesadas en el cultivo de este hongo, utilizar paja de trigo suplementada con harina de soya y como regulador de pH CaCO3, ya que en dicho sustrato se obtuvieron los mayores porcentajes de eficiencia biológica para la producción de cuerpos fructíferos de A. cylindracea.

  1. Interpain A, a cysteine proteinase from Prevotella intermedia, inhibits complement by degrading complement factor C3.

    Directory of Open Access Journals (Sweden)

    Michal Potempa

    2009-02-01

    Full Text Available Periodontitis is an inflammatory disease of the supporting structures of the teeth caused by, among other pathogens, Prevotella intermedia. Many strains of P. intermedia are resistant to killing by the human complement system, which is present at up to 70% of serum concentration in gingival crevicular fluid. Incubation of human serum with recombinant cysteine protease of P. intermedia (interpain A resulted in a drastic decrease in bactericidal activity of the serum. Furthermore, a clinical strain 59 expressing interpain A was more serum-resistant than another clinical strain 57, which did not express interpain A, as determined by Western blotting. Moreover, in the presence of the cysteine protease inhibitor E64, the killing of strain 59 by human serum was enhanced. Importantly, we found that the majority of P. intermedia strains isolated from chronic and aggressive periodontitis carry and express the interpain A gene. The protective effect of interpain A against serum bactericidal activity was found to be attributable to its ability to inhibit all three complement pathways through the efficient degradation of the alpha-chain of C3 -- the major complement factor common to all three pathways. P. intermedia has been known to co-aggregate with P. gingivalis, which produce gingipains to efficiently degrade complement factors. Here, interpain A was found to have a synergistic effect with gingipains on complement degradation. In addition, interpain A was able to activate the C1 complex in serum, causing deposition of C1q on inert and bacterial surfaces, which may be important at initial stages of infection when local inflammatory reaction may be beneficial for a pathogen. Taken together, the newly characterized interpain A proteinase appears to be an important virulence factor of P. intermedia.

  2. Las organizaciones intermedias en los procesos de innovación en México

    OpenAIRE

    Pérez Hernández, Maria del Pilar Monserrat

    2016-01-01

    Resumen: La actividad innovadora depende de los vínculos o mediación que exista entre los actores que generan la tecnología y el conocimiento y los usuarios finales de una y otro; este papel lo cumplen diversas organizaciones intermedias (OI), las cuales traducen las necesidades de los usuarios de tecnologías en desarrollo. Este trabajo analiza el papel de dichas organizaciones intermedias en los procesos de innovación en México. Así, se distingue que existen tres vacíos estructurales: en la ...

  3. Incomplete paraplegia caused by extramedullary hematopoiesis in a patient with thalassemia intermedia.

    Science.gov (United States)

    Hisamud-Din, Nurhasyimah; Mustafah, Nadia Mohd; Fauzi, Aishah Ahmad; Hashim, Natiara Mohamad

    2017-01-01

    Extramedullary hematopoiesis (EMH) is the production of blood cell precursors outside the bone marrow that occur in various hematological diseases. In patients with thalassemia intermedia, ineffective erythropoiesis drives compensatory EMH in the liver, pancreas, pleura, spleen, ribs and spine. We describe a patient with thalassemia intermedia who presented with acute neurological symptoms caused by paraspinal EMH, which responded well to combination therapy of steroid, hypertransfusion, laminectomy and excision of pseudotumor and hydroxyurea therapy to boost the formation of fetal haemoglobin. Prompt recognition of EMH based on clinical presentation and typical radiological findings should be made. Early treatment is recommended to prevent irreversible damage to the spinal cord.

  4. The mass remote sensing image data management based on Oracle InterMedia

    Science.gov (United States)

    Zhao, Xi'an; Shi, Shaowei

    2013-07-01

    With the development of remote sensing technology, getting the image data more and more, how to apply and manage the mass image data safely and efficiently has become an urgent problem to be solved. According to the methods and characteristics of the mass remote sensing image data management and application, this paper puts forward to a new method that takes Oracle Call Interface and Oracle InterMedia to store the image data, and then takes this component to realize the system function modules. Finally, it successfully takes the VC and Oracle InterMedia component to realize the image data storage and management.

  5. Prevalence of Pulmonary Hypertension in Patients with Thalassemia Intermedia in 2009: a single center's experience.

    Science.gov (United States)

    Moghaddam, Hassan Mottaghi; Badiei, Zahra; Eftekhari, Kambiz; Shakeri, Reza; Farhangi, Hamid

    2015-07-01

    There are various clinical symptoms of thalassemia intermedia, and they lie roughly between those of major and minor forms of the disease. Patients with thalassemia intermedia occasionally require blood transfusions. This renders them susceptible to pulmonary arterial hypertension (PAH) syndrome, which is one of the most significant complications in patients with thalassemia intermedia. PAH is more common in in thalassemia intermedia than in thalassemia major, and it may cause cardiac complications in patients who are older than 30. The objective of this study was to estimate the prevalence of PAH in thalassemia intermedia patients so that they can be referred expeditiously for treatment, thereby preventing the complications that occur later. This cross sectional study was conducted under the supervision of hematology department of Mashhad Medical University. Forty-one patients with thalassemia intermedia were examined at the Sarvar Thalassemia and Hemophilia Clinic of Mashhad. Electrocardiography, chest radiography, and echocardiography tests were performed for all of the patients by the same pediatric cardiologist. The data were processed by SPSS software, version 11.5, and the results were analyzed using chi-squared, Student's t, and Mann-Whitney tests. The mean age of the patients was 21.93±8.34. They had been under pediatric heart specialists' constant examination and treatment since their childhood when they were diagnosed with TI, and continue to receive regular follow-up care. The prevalence of pulmonary hypertension was 24% in our study population. In patients with thalassemia intermedia, the left ventricular (LV) mass indices were about 3-5 times higher than would be expected in a normal population. Patients with higher LV mass indices have a greater risk of developing pulmonary hypertension, and those with serum ferritin levels below 1000 ng/ml are less susceptible to diastolic dysfunction. Pulmonary hypertension is common in patients with thalassemia

  6. Las organizaciones intermedias en el campo de la responsabilidad social empresarial

    OpenAIRE

    Abelenda Casalet, Lucía

    2010-01-01

    Se presenta un estudio sobre un tipo particular de organizaciones de la sociedad civil, las organizaciones intermedias. Focalizamos el estudio en las organizaciones intermedias que se desempeñan en el campo de la Responsabilidad Social Empresarial. La investigación está orientada a la búsqueda de rasgos que puedan definir la acción de estas organizaciones y su capacidad de innovación social en un campo particular. En el desarrollo de la investigación se jerarquizó el nivel micro, relacionado ...

  7. Toxicidad biológica de cepas nativas de Bacillus thuringiensis Berliner en larvas de Tecia solanivora Povolny

    Directory of Open Access Journals (Sweden)

    Paola Martínez

    2011-11-01

    Full Text Available La biodiversidad microbiológica de los suelos del departamento de Boyacá aún no ha sido explorada en toda su magnitud y existen microorganismos, como en el caso de Bacillus thuringiensis Berliner (Bt, que pueden emplearse para el desarrollo de estrategias biológicas de control de plagas en el futuro. Por lo anterior, el presente trabajo evaluó la actividad biológica, expresada como  toxicidad, de cepas nativas de B. thuringiensis en la Polilla Guatemalteca  de  la  papa  Tecia  solanivora Povolny, una de  las plagas más  limitantes en el cultivo de papa en la región andina colombiana. Esta  evaluación  se  realizó con  aislados de  Bt obtenidos y conservados por el Grupo de Manejo Biológico de Cultivos (GMBC en el Laboratorio de Control Biológico de la UPTC, colectados de muestras de  suelo en  la Provincia Centro de Boyacá y  previamente  caracterizados  macro  y microscópicamente.  La   toxicidad  se  evaluó mediante bioensayos con larvas de primer instar de  T.  solanivora,  utilizando  el método  decontaminación superficial de cubos de papa. Se evaluó  la mortalidad  ocho  días  después  de montado  el  bioensayo  y  se  determinó, igualmente,  la Concentración letal media (Cl 50 de las cepas que presentaron  la mayor actividad  tóxica en  los  bioensayos  iniciales.  Los  aislamientosGMBC-B054, GMBC-B071, GMBC-B076, GMBC- B098, GMBC-B111 y GMBC-B117 fueron  los más activos, con Cl 50 de 1.08x106, 4.24x106, 5.12x106, 4.36x103, 3.56x103  y  1.19x104  esporas  · mL-1,respectivamente.

  8. El anticlinal y las discordancias de Pareja (Guadalajara): definición de las unidades cenozoicas de la Depresión Intermedia (provs. Cuenca y Guadalajara, España)

    OpenAIRE

    Torres Pérez-Hidalgo, Trinidad José

    2006-01-01

    Este trabajo contempla las unidades representadas en la sección estratigráfica del Anticlinal la Pareja (Guadalajara, España) en la cuenca cenozoica denominada Depresión Intermedia. La columna estratigráfica de la zona permite identificar tres unidades principales separadas por discordancias angulares relacionables con fases tectónicas de la orogenia alpina. También se han diferenciado subunidades que, en su mayor parte, vienen definidas por rupturas sedimentarias ligadas a proces...

  9. La ordenación territorial en el País Vasco. Debilidades, amenazas,fortalezas y oportunidades de la escala intermedia

    Directory of Open Access Journals (Sweden)

    Pedro J. Lozano Valencia

    2003-01-01

    Full Text Available La ordenación del territorio encuentra su razón de ser, en el País Vasco, a través de las Directrices de Ordenación y dos tipos diferentes de documentos: los Planes Territoriales Parciales y los Planes Territoriales Sectoriales. Una vez delimitadas las comarcas y los sectores estratégicos, se aplican dichos documentos. Con todo, se logra la coordinación de las Directivas europeas a escala superior, y los Planes y Normas Subsidiarias de Ordenación Urbana a una escala inferior o local. En medio quedan todas esas herramientas de ordenación mencionadas anteriormente, que conforman lo que se ha venido a definir como la escala intermedia. Ésta aparece como un marco muy apropiado para abordar las debilidades, amenazas, fortalezas y oportunidades que, en un horizonte de 16 a 20 años, presenta el territorio vasco

  10. El patrimonio modesto de las ciudades intermedias bonaerenses: prácticas usuarias y reflexiones hacia la preservación de las casas "chorizo" de Tandil

    Directory of Open Access Journals (Sweden)

    Lorena Marina Sánchez

    2014-12-01

    Por ello, la indagación se enfoca en el conocimiento del estado de concientización de los usuarios de estas viviendas en la ciudad intermedia de Tandil, cuyo pasado ha forjado un destacado corpus patrimonial modesto que subsiste dentro de la actual dinámica urbana. Se trabaja desde una concepción de la investigación principalmente cualitativa, con ayuda de datos cuantitativos, centrada en la variable histórica urbana-arquitectónica, la social y sus relaciones. Los resultados generales alcanzados, que hacen hincapié en las modificaciones de las viviendas, han posibilitado reflexiones orientadas hacia su salvaguarda.

  11. EFECTO DE LA ADICIÓN DE CEPAS PROBIÓTICAS SOBRE METABOLITOS SANGUÍNEOS EN CERDOS EN CRECIMIENTO

    Directory of Open Access Journals (Sweden)

    SANTIAGO LONDOÑO-PÉREZ

    Full Text Available El destete es la fase más crítica del cerdo, produciendo trastornos intestinales y diarreas. Para prevenirlo, se han utilizado antibióticos promotores de crecimiento (APC, generando residuos medicamentosos en el producto final. Como alternativa, se utilizan bacterias probióticas que favorecen la síntesis proteica y el metabolismo lipídico. El objetivo fue identificar cambios en metabolitos plasmáticos en cerdos posdestete que consumieron diferentes cepas probióticas. 80 lechones (destetados a 21 días consumieron dos dietas: dieta comercial con y sin la adición de antibiótico (con adición de probióticos L. casei, L. acidophilus o E. faecium en el agua. Se tomaron muestras sanguíneas (días 15, 30 y 45 posdestete para determinar cambios plasmáticos de metabolitos. Se observó un aumento (P<0,05 en niveles plasmáticos de Calcio, Fósforo, glucosa y Fosfatasa alcalina; además, disminuyeron los niveles de Creatinina, triglicéridos y ALT (alanino-transferasa en animales que consumieron E. faecium, comparándolos con los que consumieron antibiótico. Los probióticos (especialmente E. faecium, pueden ser considerados como una alternativa al uso de APC en dietas de cerdos, pues mejoran el estado de órganos digestivos y actúan como promotores naturales del crecimiento ya que mejoran el estado metabólico del animal con cambios positivos en los niveles sanguíneos de Calcio, Fósforo y Glucosa.

  12. Micropropagation of onion (Allium cepa L.) from immature inflorescences.

    Science.gov (United States)

    Marinangeli, Pablo

    2013-01-01

    In vitro plant production by direct organogenesis from immature flower heads is an ideal approach for clonal propagation of onions (Allium cepa L.). This technique ensures genetic stability, high propagation rate, and maintains donor plant of explants with an advantage over other means of in vitro regeneration. Onion micropropagation is usually applied in breeding programs, maintenance, and multiplication of cytoplasmic-male sterile lines for hybrid production, germplasm conservation, and as a tool for the application of other biotechnologies. For in vitro culture, mature onion bulbs are induced to reproductive phase by vernalization and forced to inflorescence initiation. Immature umbels are dissected from bulbs or cut directly when they appear from the pseudostem among the leaves. Disinfected inflorescences are cultivated in BDS basal medium supplemented with 30 g/L sucrose, 0.1 mg/L naphthalene acetic acid, 1 mg/L N (6)-benzyladenine, and 8 g/L agar, pH 5.5, under 16 h photoperiod white fluorescent light (PPD: 50-70 μmol/m(2)s) for 35 days. The regenerated shoot clumps are divided and subculture under the same conditions. For bulbification phase, the individual shoots are cultured in BDS basal medium containing 90 g/L sucrose, without plant growth regulators, pH 5.5, under 16 h photoperiod. Microbulbs can be directly cultivated ex vitro without acclimation.

  13. CEPA calculations on open-shell molecules. Pt. 11

    International Nuclear Information System (INIS)

    Staemmler, V.

    1990-01-01

    Ab initio calculations at SCF and CEPA levels using large Gaussian basis sets have been performed for the two lowest electronic states, X 2 Σ + and A 2 Π, of HeAr + . Spin-orbit coupling (SOC) effects have been added using a semiempirical treatment. The resulting potential curves for the three states X, A 1 , and A 2 have been used to evaluate molecular constants such as vibrational intervals ΔG(υ+1/2) and rotational constants B υ as well as - by means of a Dunham expansion - equilibrium constants such as R e , ω e , B e etc. Comparison with the experimental data from UV emission spectroscopy shows that the calculated potential curves are slightly too shallow and have too large equilibrium distances: D e =242 cm -1 and R e =2.66 A compared to the experimental values of 262 cm -1 and 2.585 A, respectively, for the X 2 Σ + ground state. However, the ab initio calculations yield more bound vibrational levels than observed experimentally and allow for a more complete Dunham analysis, in particular for the A 2 state. The experimental value of 154 cm -1 for the dissociation energy D e of this state is certainly too low; our best estimate is 180±5 cm -1 . For the A 1 state our calculations are predictions since this state has not yet been observed experimentally. (orig.)

  14. Jóvenes sin vínculos. El papel de las estructuras intermedias en un espacio urbano desfavorecido

    Directory of Open Access Journals (Sweden)

    Cecilia Eseverri Mayer

    2015-01-01

    Full Text Available Este artículo describe el proceso de desafiliación (Castel, 1995 juvenil, tomando como caso de estudio un barrio desfavorecido de Madrid. A través de una metodología etnográfica examina el efecto de las estructuras intermedias (la familia, la comunidad local y las asociaciones civiles en la reinserción educativa y laboral de los jóvenes de origen marroquí, dominicano y ecuatoriano. La investigación señala como elemento más determinante de dicha reinserción el capital humano de los padres, confi rmando los hallazgos de la sociología de la educación (Bourdieu, 1970. Además, advierte del doble efecto (positivo y negativo que provoca la influencia de las comunidades étnicas en los jóvenes más vulnerables y demuestra el rol crucial que cumple el tejido asociativo. Estas organizaciones, herederas de las movilizaciones de los años 70, tienen la capacidad de abrir nuevas vías de inserción a través de la colaboración con las administraciones, los grupos étnicos y las familias.

  15. A comparison of heart function and arrhythmia in clinically asymptomatic patients with beta thalassemia intermedia and beta thalassemia major.

    Science.gov (United States)

    Amoozgar, Hamid; Zeighami, Samaneh; Haghpanah, Sezaneh; Karimi, Mehran

    2017-01-01

    The goal of this study was to compare heart function and arrhythmia in clinically asymptomatic patients with beta thalassemia intermedia and beta thalassemia major. In this cross-sectional study, 60 patients with beta thalassemia major and 60 patients with beta thalassemia intermedia who had clinically no symptoms of arrhythmia and clinically normal heart function were evaluated using 24-hour ambulatory electrocardiogram monitoring and echocardiography. For data analysis SPSS ver.20 software was used. A P-value of less than 0.05 was considered statistically significant. The mean age of the beta thalassemia intermedia patients was 24.18 ± 7.9 years and the mean age in beta thalassemia major was 24.38 ± 7.7 years (P>0.05). Premature atrial contractions (PACs) were observed in 14 (23.3%) patients with beta thalassemia intermedia and in 22 (36.6%) beta thalassemia major patients. Premature ventricular contractions (PVCs) were detected in 8 (13.3%) patients in the beta thalassemia intermediate group and 16 (26.6) patients in the beta thalassemia major group, respectively. The left ventricular diastolic dimension, end-diastolic volume, and stroke volume were significantly higher in beta thalassemia intermedia group (Pintermedia group. Both atrial and ventricular arrhythmias were more common in the beta thalassemia major group. Higher end-diastolic volume and stroke volume were detected in the beta thalassemia intermedia group. Pulmonary acceleration time was lower in the beta thalassemia intermedia group, which can be an indicator of higher pulmonary pressure.

  16. Comparison of the virulence of exopolysaccharide-producing Prevotella intermedia to exopolysaccharide non-producing periodontopathic organisms.

    Science.gov (United States)

    Yamanaka, Takeshi; Yamane, Kazuyoshi; Furukawa, Tomoyo; Matsumoto-Mashimo, Chiho; Sugimori, Chieko; Nambu, Takayuki; Obata, Noboru; Walker, Clay B; Leung, Kai-Poon; Fukushima, Hisanori

    2011-08-25

    Evidence in the literature suggests that exopolysaccharides (EPS) produced by bacterial cells are essential for the expression of virulence in these organisms. Secreted EPSs form the framework in which microbial biofilms are built. This study evaluates the role of EPS in Prevotella intermedia for the expression of virulence. This evaluation was accomplished by comparing EPS-producing P. intermedia strains 17 and OD1-16 with non-producing P. intermedia ATCC 25611 and Porphyromonas gingivalis strains ATCC 33277, 381 and W83 for their ability to induce abscess formation in mice and evade phagocytosis. EPS-producing P. intermedia strains 17 and OD1-16 induced highly noticeable abscess lesions in mice at 107 colony-forming units (CFU). In comparison, P. intermedia ATCC 25611 and P. gingivalis ATCC 33277, 381 and W83, which all lacked the ability to produce viscous materials, required 100-fold more bacteria (109 CFU) in order to induce detectable abscess lesions in mice. Regarding antiphagocytic activity, P. intermedia strains 17 and OD1-16 were rarely internalized by human polymorphonuclear leukocytes, but other strains were readily engulfed and detected in the phagosomes of these phagocytes. These results demonstrate that the production of EPS by P. intermedia strains 17 and OD1-16 could contribute to the pathogenicity of this organism by conferring their ability to evade the host's innate defence response.

  17. Development of Prevotella intermedia-specific PCR primers based on the nucleotide sequences of a DNA probe Pig27.

    Science.gov (United States)

    Kim, Min Jung; Hwang, Kyung Hwan; Lee, Young-Seok; Park, Jae-Yoon; Kook, Joong-Ki

    2011-03-01

    The aim of this study was to develop Prevotella intermedia-specific PCR primers based on the P. intermedia-specific DNA probe. The P. intermedia-specific DNA probe was screened by inverted dot blot hybridization and confirmed by Southern blot hybridization. The nucleotide sequences of the species-specific DNA probes were determined using a chain termination method. Southern blot analysis showed that the DNA probe, Pig27, detected only the genomic DNA of P. intermedia strains. PCR showed that the PCR primers, Pin-F1/Pin-R1, had species-specificity for P. intermedia. The detection limits of the PCR primer sets were 0.4pg of the purified genomic DNA of P. intermedia ATCC 49046. These results suggest that the PCR primers, Pin-F1/Pin-R1, could be useful in the detection of P. intermedia as well as in the development of a PCR kit in epidemiological studies related to periodontal diseases. Crown Copyright © 2010. Published by Elsevier B.V. All rights reserved.

  18. Taxonomic study on Japanese Salvia (Lamiaceae): Phylogenetic position of S. akiensis, and polyphyletic nature of S. lutescens var. intermedia.

    Science.gov (United States)

    Takano, Atsuko

    2017-01-01

    Both Salvia akiensis and S. lutescens (Lamiaceae) are endemic to Japan. Salvia akiensis was recently described in 2014 in the Chugoku (= SW Honshu) region, and each four varieties of S. lutescens distributed allopatrically. Among varieties in S. lutescens , var. intermedia show a disjunctive distribution in the Kanto (=E Honshu) and Kinki (= W Honshu) regions. Recent field studies of S. lutescens var. intermedia revealed several morphological differences between the Kanto and Kinki populations. Here, I evaluated these differences among Salvia lutescens var. intermedia and its allies with morphological analysis and molecular phylogenetic analyses of nuclear ribosomal DNA (internal and external transcribed spacer regions) and plastid DNA ( ycf1-rps15 spacer, rbcL , and trnL-F ) sequences. Both morphological analysis and molecular phylogenetic analyses showed that S. lutescens var. intermedia from the Kinki region and var. lutescens were closely related to each other. However, var. intermedia from the Kanto region exhibited an association with S. lutescens var. crenata and var. stolonifera, which also grew in eastern Japan, rather than var. intermedia in the Kinki region. These results indicated that S. lutescens var. intermedia is not a taxon with a disjunctive distribution, but a combination of two or more allopatric taxa. Present study also suggested that S. akiensis was most closely related to S. omerocalyx .

  19. Experimental weed control of Najas marina ssp. intermedia and Elodea nuttallii in lakes using biodegradable jute matting

    Directory of Open Access Journals (Sweden)

    Markus A. Hoffmann

    2013-10-01

    Full Text Available The use of jute matting in managing the invasive aquatic macrophyte species Elodea nuttallii (Planch. H. St. John and Najas marina ssp. intermedia (Wolfg. ex Gorski Casper (Najas intermedia was studied in laboratory experiments and field trials. Four German lakes with predominant population of Najas intermedia or Elodea nuttalli were chosen for the experiment and areas between 150 and 300 m² were covered with jute textile. The effect of the matting on the growth of invasive and non-invasive macrophytes was determined through comparison with control transects. Biodegradable jute matting successfully suppressed the invasive macrophyte Najas intermedia and significantly reduced the growth of Elodea nuttalli in lakes. The results indicate that the capability of the matting to inhibit the growth of Elodea nuttallii and Najas intermedia depends on the mesh size of the jute weaving and that environmental conditions can affect its efficiency. Various indigenous species like Charales or Potamogeton pusillus L. were able to grow through the jute fabric and populate the treated areas. Until the end of the vegetation period, none of the invasive species were able to penetrate the covering and establish a stable population; in fact, in the subsequent year the jute matting affected only the spread of Najas intermedia. Jute matting proved to be an easy-to-use and cheap method to control the growth of Elodea nuttallii and Najas intermedia.

  20. Evaluation of the efficacy of vacuum cleaners for the integrated control of brown spider Loxosceles intermedia

    Directory of Open Access Journals (Sweden)

    E. N. Ramires

    2007-01-01

    Full Text Available Some venomous spiders of the genus Loxosceles can reach high population densities inside and around houses. In Brazil, most spider accidents are related to Loxosceles intermedia. Control of loxoscelism should utilize integrated pest management tools, such as vacuum cleaners, to eliminate egg sacs, webs and spiders. The present study tested the efficacy of one type of vacuum cleaner (for professional and domestic use in the control of L. intermedia populations. Cockroaches (Pycnoscelus surinamensis were used in some tests for comparison. Vacuuming using standard accessories or a paper tube resulted in the death of all female (n=60, male (n=60, young (n=60 and just-hatched (n=60 L. intermedia, and all egg sacs (n=5 were destroyed. The removal of the plastic plate present at the bottom of the vacuuming tube inside the machine allowed some spiders to survive the vacuuming process. When kept inside a vacuum bag full of dust and debris, adult females (n=10 survived for 10 days; however, significant mortality was observed among male (n=10 and young individuals (n=10. Addition of cornstarch to the vacuum bag did not affect the spiders (n=20. Vacuum cleaners, such as the one used in the present investigation, are promising tools for integrated management of L. intermedia and other spiders in domestic environments.

  1. Composition, Cytotoxic and Antimicrobial Activities of Satureja intermedia C.A.Mey Essential Oil

    Directory of Open Access Journals (Sweden)

    Javad Sharifi-Rad

    2015-08-01

    Full Text Available In this study, the essential oil (EO constituents from the aerial parts of Satureja intermedia C.A.Mey were detected by GC and GC/MS. The antimicrobial activity of EO on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. Thirty-nine compounds were identified and the main EO constituents were γ-terpinene (37.1%, thymol (30.2%, p-cymene (16.2%, limonene (3.9%, α-terpinene (3.3%, myrcene (2.5%, germacrene B (1.4%, elemicine (1.1% and carvacrol (0.5%. The S. intermedia EO showed a concentration-dependent decrease in viability of Hep-G2 (hepatocellular carcinoma and MCF-7 (breast adenocarcinoma human cancer cell lines (p < 0.05. Antimicrobial screening of S. intermedia EO demonstrated slight antibacterial and antifungal activities against Streptococcus mutants, S. salivarius, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and C. glabrata. Further preclinical studies are needed to assess the efficacy and safety of S. intermedia EO as a new promising anticancer agent.

  2. Rhamnus intermedia Steud. et Hochst. - a New Source of Bioactive Phytochemicals

    Czech Academy of Sciences Publication Activity Database

    Samec, D.; Kremer, D.; Grúz, Jiří; Grubešić, R. J.; Piljac-Žegarac, J.

    2012-01-01

    Roč. 85, č. 2 (2012), s. 125-129 ISSN 0011-1643 R&D Projects: GA AV ČR KAN200380801 Institutional research plan: CEZ:AV0Z50380511 Keywords : Rhamnus intermedia * antioxidant activity * total phenols Subject RIV: EC - Immunology Impact factor: 0.614, year: 2012

  3. Polymorphism for pKALILO based senescence in Hawaiian populations of Neurospora intermedia and Neurospora tetrasperma.

    NARCIS (Netherlands)

    Maas, M.F.P.M.; Mourik, van A.; Hoekstra, R.F.; Debets, A.J.M.

    2005-01-01

    The natural population of Neurospora intermedia from Hawaii is polymorphic for the presence of the linear mitochondrial plasmid pKALILO that is associated with an infectious senescence syndrome. Although inter-specific horizontal transmission is experimentally possible, thus far pKALILO associated

  4. Intermedia: A System for Linking Multimedia Documents. IRIS Technical Report 86-2.

    Science.gov (United States)

    Yankelovich, Nicole

    "Intermedia" is a hypermedia system which was developed for use in university research and teaching. It provides a framework for object-oriented, direct manipulation editors and applications, and the capability to link together materials created with those applications. Instructors are able to construct exploratory environments for their…

  5. Formation of Lignans(-)-Secoisolariciresinol and (-)-Matairesinol with Forsythia intermedia Cell-Free Extracts

    Science.gov (United States)

    Umezawa, Toshiaki; Davin, Laurence B.; Lewis, Norman G.

    1991-01-01

    In vivo labeling experiments of Forsythia intermedia plant tissue with [8-(C-14)]- and [9,9-(2)H2,OC(2)H3]coniferyl alcohols revealed that the lignans, (-)-secoisolariciresinol and (-)-matairesinol, were derived from two coniferyl alcohol molecules; no evidence for the formation of the corresponding (+)-enantiomers was found. Administration of (+/-)-[Ar-(H-3)] secoisolariciresinols to excised shoots of F.intermedia resulted in a significant conversion into (-)-matairesinol; again, the (+)-antipode was not detected. Experiments using cell-free extracts of F.intermedia confirmed and extended these findings. In the presence of NAD(P)H and H2O2, the cell-free extracts catalyzed the formation of (-)- secoisolariciresinol, with either [8-(C-14)]- or [9,9-(2)H2,OC(2)H3]coniferyl alcohols as substrates. The (+)- enantiomer was not formed. Finally, when either (-)-[Ar-(H-3)] or (+/-)-[Ar-(H-2)]secoisolariciresinols were used as substrates, in the presence of NAD(P), only (-)- and not (+)-matairesinol formation occurred. The other antipode, (+)-secoisolariciresinol, did not serve as a substrate for the formation of either (+)- or (-)-matairesinol. Thus, in F.intermedia, the formation of the lignan, (-)-secoisolariciresinol, occurs under strict stereochemical control, in a reaction or reactions requiring NAD(P)H and H2O2 as cofactors. This stereoselectivity is retained in the subsequent conversion into (-)-matairesinol, since (+)-secoisolariciresinol is not a substrate. These are the first two enzymes to be discovered in lignan formation.

  6. Issues in Designing a Hypermedia Document System: The Intermedia Case Study.

    Science.gov (United States)

    Yankelovich, Nicole; And Others

    1986-01-01

    Intermedia, a hypermedia system developed at Brown University's Institute for Research (Rhode Island) in Information and Scholarship, is first described, and then used as a case study to explore a number of key issues that software designers must consider in the development of hypermedia document systems. A hypermedia document system is defined as…

  7. Reconstruction of chromosome rearrangements between the two most ancestral duckweed species Spirodela polyrhiza and S. intermedia.

    Science.gov (United States)

    Hoang, Phuong T N; Schubert, Ingo

    2017-12-01

    The monophyletic duckweeds comprising five genera within the monocot order Alismatales are neotenic, free-floating, aquatic organisms with fast vegetative propagation. Some species are considered for efficient biomass production, for life stock feeding, and for (simultaneous) wastewater phytoremediation. The ancestral genus Spirodela consists of only two species, Spirodela polyrhiza and Spirodela intermedia, both with a similar small genome (~160 Mbp/1C). Reference genome drafts and a physical map of 96 BACs on the 20 chromosome pairs of S. polyrhiza strain 7498 are available and provide useful tools for further evolutionary studies within and between duckweed genera. Here we applied sequential comparative multicolor fluorescence in situ hybridization (mcFISH) to address homeologous chromosomes in S. intermedia (2n = 36), to detect chromosome rearrangements between both species and to elucidate the mechanisms which may have led to the chromosome number alteration after their evolutionary separation. Ten chromosome pairs proved to be conserved between S. polyrhiza and S. intermedia, the remaining ones experienced, depending on the assumed direction of evolution, translocations, inversion, and fissions, respectively. These results represent a first step to unravel karyotype evolution among duckweeds and are anchor points for future genome assembly of S. intermedia.

  8. The contingency of intermedia agenda setting: a longitudinal study in Belgium

    NARCIS (Netherlands)

    Vliegenthart, R.; Walgrave, S.

    2008-01-01

    This large-scale study investigates how intermedia agenda-setting effects are moderated by five factors: (1) lag length; (2) medium type; (3) language/institutional barriers; (4) issue type; and (5) election or non-election context. Longitudinal analyses of daily attention to twenty-five issues in

  9. Composition, Cytotoxic and Antimicrobial Activities of Satureja intermedia C.A.Mey Essential Oil.

    Science.gov (United States)

    Sharifi-Rad, Javad; Sharifi-Rad, Mehdi; Hoseini-Alfatemi, Seyedeh Mahsan; Iriti, Marcello; Sharifi-Rad, Majid; Sharifi-Rad, Marzieh

    2015-08-03

    In this study, the essential oil (EO) constituents from the aerial parts of Satureja intermedia C.A.Mey were detected by GC and GC/MS. The antimicrobial activity of EO on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. Thirty-nine compounds were identified and the main EO constituents were γ-terpinene (37.1%), thymol (30.2%), p-cymene (16.2%), limonene (3.9%), α-terpinene (3.3%), myrcene (2.5%), germacrene B (1.4%), elemicine (1.1%) and carvacrol (0.5%). The S. intermedia EO showed a concentration-dependent decrease in viability of Hep-G2 (hepatocellular carcinoma) and MCF-7 (breast adenocarcinoma) human cancer cell lines (p intermedia EO demonstrated slight antibacterial and antifungal activities against Streptococcus mutants, S. salivarius, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and C. glabrata. Further preclinical studies are needed to assess the efficacy and safety of S. intermedia EO as a new promising anticancer agent.

  10. Identification and functional analysis of the gene cluster for fructan utilization in Prevotella intermedia.

    Science.gov (United States)

    Fuse, Haruka; Fukamachi, Haruka; Inoue, Mitsuko; Igarashi, Takeshi

    2013-02-25

    Fructanase enzymes hydrolyze the β-2,6 and β-2,1 linkages of levan and inulin fructans, respectively. We analyzed the influence of fructan on the growth of Prevotella intermedia. The growth of P. intermedia was enhanced by addition of inulin, implying that P. intermedia could also use inulin. Based on this finding, we identified and analyzed the genes encoding a putative fructanase (FruA), sugar transporter (FruB), and fructokinase (FruK) in the genome of strain ATCC25611. Transcript analysis by RT-PCR showed that the fruABK genes were co-transcribed as a single mRNA and semi-quantitative analysis confirmed that the fruA gene was induced in response to fructose and inulin. Recombinant FruA and FruK were purified and characterized biochemically. FruA strongly hydrolyzed inulin, with slight degradation of levan via an exo-type mechanism, revealing that FruA is an exo-β-d-fructanase. FruK converted fructose to fructose-6-phosphate in the presence of ATP, confirming that FruK is an ATP-dependent fructokinase. These results suggest that P. intermedia can utilize fructan as a carbon source for growth, and that the fructanase, sugar transporter, and fructokinase proteins we identified are involved in this fructan utilization. Copyright © 2012 Elsevier B.V. All rights reserved.

  11. Isolation of Yersinia enterocolitica and Y. intermedia from fatal cases of diarrhoeal illness in Bangladesh

    NARCIS (Netherlands)

    Butler, T.; Islam, M.; Islam, M. R.; Azad, A. K.; Huq, M. I.; Speelman, P.; Roy, S. K.

    1984-01-01

    From three fatal cases of diarrhoeal illness in Bangladesh, Yersinia species were isolated from tissues at post-mortem examination. One patient was infected with Y. enterocolitica serotype 0:7, 8 and two patients were infected with Y. intermedia. These patients were infected also with other enteric

  12. Evaluación de la tolerancia a la crioconservación de dos cepas de Escherichia coli K12 de uso frecuente en biotecnología

    Directory of Open Access Journals (Sweden)

    Diliana Celeste Pérez-Reytor*, Angela Estela Sosa Espinosa

    2010-08-01

    Full Text Available Uno de los métodos más recomendados para la conservación de E. coli es la congelación entre -20ºC y -70ºC, donde comúnmente se utiliza glicerol o dimetilsulfóxido como criopreservante. Los bancos de E. coli que se conservan por esta vía pueden mantener altas viabilidades por más de 10 años. A pesar de que es una práctica común están poco documentadas las diferencias que existen entre las cepas de interés biotecnológico en cuanto a la resistencia a la congelación. En este artículo se comparan dos cepas mutantes de E. coli K12: la cepa RRI y la cepa HB101, con el objetivo de evaluar el comportamiento de ambas a dos temperaturas de almacenamiento y diferentes concentraciones de glicerol, después de ocho ciclos sucesivos de congelación-descongelación. Las cepas estudiadas sólo difieren en su capacidad de reparación al daño sobre el ADN al presentar la HB101 una variante mutada del gen recA. Cuando se utilizó una temperatura de almacenamiento de -70 ºC y diferentes concentraciones de criopreservante las cepas tuvieron un comportamiento similar de supervivencia, después de los ocho ciclos. Sin embargo, el comportamiento de la supervivencia difiere cuando la temperatura de congelación en los ciclos es de -20 ºC. Además de la supervivencia y la estabilidad de los marcadores genéticos estudiados, observamos que el efecto de los ciclos de congelación y descongelación retardó el tiempo de aparición de las colonias en medio sólido.

  13. Tipificación molecular de Brucella abortus cepa 19 y su aplicación al control de biológicos Molecular characterization of Brucella abortus strain 19 and its application for controlling biologics

    Directory of Open Access Journals (Sweden)

    M.E. Pavan

    2005-09-01

    Full Text Available Brucella abortus es el agente etiológico de la brucelosis bovina. La cepa 19, utilizada en la elaboración de vacunas, puede ser identificada a través de una deleción en la región eri asociada con la sensibilidad al eritritol. Se optimizó un ensayo de PCR para caracterizar específicamente esta cepa. El método que describimos es un procedimiento rápido para identificar B. abortus y simultáneamente diferenciar la cepa 19 de otras cepas de B. abortus biovar 1. Hemos aplicado este ensayo para la detección de la cepa 19 en vacunas contra la brucelosis bovina elaboradas en Argentina. Los resultados indican que este método podría ser útil para el seguimiento de las cepas madres y semillas utilizadas en la producción industrial de esta vacuna. Esta metodología también contribuiría a la reducción del riesgo de la infección adquirida en el laboratorio y podría aplicarse como prueba de rutina para confirmar la presencia de B. abortus en vacunas no relacionadas.Brucella abortus is the etiological agent of bovine brucellosis. The strain 19 used in vaccine elaboration can be identified through a deletion in the eri region associated with its susceptibility to erythritol. We optimized a PCR assay for specific characterization of this strain. The method described here is a rapid procedure that enables identification of B. abortus, and simultaneous differentiation of the strain 19 from other B. abortus biovar 1 strains. We applied the assay to detect the strain 19 in vaccines against B. abortus produced in Argentina. The results show this method could be used to follow vaccine seed cultures of this strain. The methodology could also contribute to reduce the risk of a laboratory-acquired infection and could be of great help as a routine test for confirmation of B. abortus in non related vaccines.

  14. Relative contribution of Prevotella intermedia and Pseudomonas aeruginosa to lung pathology in airways of patients with cystic fibrosis.

    Science.gov (United States)

    Ulrich, Martina; Beer, Isabelle; Braitmaier, Peter; Dierkes, Michaela; Kummer, Florian; Krismer, Bernhard; Schumacher, Ulrike; Gräpler-Mainka, Ute; Riethmüller, Joachim; Jensen, Peter Ø; Bjarnsholt, Thomas; Høiby, Niels; Bellon, Gabriel; Döring, Gerd

    2010-11-01

    Patients with cystic fibrosis (CF) with Pseudomonas aeruginosa lung infections produce endobronchial mucus plugs allowing growth of obligate anaerobes including Prevotella spp. Whether obligate anaerobes contribute to the pathophysiology of CF lung disease is unknown. The virulence of Prevotella intermedia and Ps aeruginosa was investigated in vitro and in mice, antibodies against P intermedia in CF sera were assessed and a culture-independent detection method for P intermedia/P nigrescens in CF sputum was tested. P intermedia reached cell numbers of >10(5)->10(7) colony-forming units (CFU)/ml sputum. The majority of patients with CF (16/17; 94.1%) produced antibodies against two immunoreactive antigens of P intermedia. Culture supernatant fluids, collected from 10(9) P intermedia cells, were more cytotoxic to respiratory epithelial cells in vitro and inflammatory in mouse lungs than respective fluids from anaerobically grown Ps aeruginosa, while fluids from aerobically grown Ps aeruginosa had the highest cytotoxicity and inflammation. Both pathological effects were largely reduced when culture supernatant fluids from 10(7) cells of either species were used. P intermedia cells (∼10(6)CFU/lung) did not induce mortality in the agar beads lung infection mouse model, while Ps aeruginosa cells caused death in 30% of mice due to rapid multiplication. A P intermedia/P nigrescens-specific PNA probe was significantly more sensitive than culture-dependent diagnostic assays to detect these strict anaerobes. Ps aeruginosa and P intermedia become significantly virulent in vitro and in vivo when cell numbers exceed 10(8) CFU/lung.

  15. Microstructure characterization of onion (A.cepa) peels and thin films for dye sensitized solar cells

    Science.gov (United States)

    Abodunrin, T.; Boyo, A.; Usikalu, M.; Obafemi, L.; Oladapo, O.; Kotsedi, L.; Yenus, Z.; Maaza, M.

    2017-03-01

    A.cepa peels are obtained from mature onion bulbs. Because of the continuous need for energy, alternative avenues for producing energy are gaining importance. The motivation for this work is based on an urgent need to source energy from readily available waste materials like domestic onion peels. Dye sensitized solar cells (DSSCs) fabricated via doctor blade method and high temperature sintering from waste (onion peels) are investigated for their ability to convert solar to electrical energy. The charge carriers were revealed under phytochemical screening. Functional groups of compounds present in A.cepa peel were analyzed with Fourier transform in infrared (FTIR). The influence of different electrolyte sensitizer is observed on the DSSCs under standard air mass conditions of 1.5 AM. The microstructure properties of these A.cepa DSSCs were explored using scanning electron microscope with energy dispersive spectroscopy (SEM/EDS), x-ray diffraction and Fluorecence spectroscopy (XRF). The interfacial boundary between A.cepa dye, TiO2 framework of TiO2 and indium doped tin oxide (ITO) reveals several prominent anatase and rutile peaks. Photoelectric results, revealed dye-sensitized solar cells with a maximum power output of 126 W and incident photon to conversion energy (IPCE) of 0.13%.This work has established that A.cepa peels can be used as a source of micro-energy generation.

  16. Calibración in situ del sensor cosmos para determinar humedad del suelo en escalas intermedias (~1 km

    Directory of Open Access Journals (Sweden)

    Fidencio Cruz Bautista

    2013-10-01

    Full Text Available La heterogeneidad del suelo influye ampliamente en el contenido de humedad, dificultando la precisa determinación de este parámetro en estudios con fines hidrológicos y ecológicos que requieren de mediciones continuas y representativas para escalas intermedias (~1 km. En este contexto un sensor de neutrón de rayo cósmico The COsmic-ray Soil Moisture Observing System (COSMOS permite cuantificar humedad del suelo de manera continua y a escalas espaciales de cientos de metros. El objetivo de este estudio fue evaluar un esquema de calibración para un sensor COSMOS CRS-1000. El estudio se realizó en una sabana de zacate buffel (Pennisetum ciliare en Rayón Sonora, México. En este sitio se instaló el COSMOS CRS-1000 y para su calibración se realizaron muestreos de suelo en dos etapas. A estas muestras se les determinó el contenido de humedad y su densidad aparente por técnicas gravimétricas. Con el contenido de humedad de estas muestras, expresado en términos volumétricos, se obtuvo por aproximación el parámetro de calibración para el COSMOS CRS-1000. El valor obtenido para este parámetro fue de 4121 conteos por hora (tasa de conteo del neutrón sobre suelo. Con este valor se realizó la corrección a los valores estimados originalmente por el sensor COSMOS CRS-1000. Al realizar esta corrección, se observó un incremento en el contenido de humedad del suelo de 1 a 2 % con respecto a los valores estimados con el COSMOS CRS-1000 en todo el periodo de análisis. A pesar de la variabilidad espacial en el contenido de humedad del suelo bajo estudio, se observó que el sensor COSMOS CRS-1000 tiene la capacidad de proveer estimaciones razonables del contenido de la humedad del suelo de manera continua a una profundidad de 0 a 40 cm, en una superficie de alrededor de 30 ha.

  17. Prueba de sensibilidad antimicrobiana de cepas de Salmonella grupo D (móviles e inmóviles aisladas de ponedoras comerciales en Colombia

    Directory of Open Access Journals (Sweden)

    J. Jaime

    2010-12-01

    Full Text Available Las infecciones originadas por bacterias del género Salmonella son una de las principalescausas de pérdidas económicas en la industria avícola, se caracterizan generalmentepor la presentación de cuadros diarreicos y septicémicos que llevan a las aves a unamarcada disminución en la producción y a la muerte. En Colombia, debido al efectonegativo que produce Salmonella spp. en las aves, y con el objetivo de poder controlarla enfermedad, se utiliza una gran variedad de productos antimicrobianos, de loscuales no se posee suficiente información acerca de su comportamiento en cuanto asensibilidad y resistencia frente a las cepas de Salmonella spp. de campo. El objetivo deeste estudio fue determinar la respuesta de 20 cepas de Salmonella grupo D (móviles einmóviles aisladas de aves ponedoras comerciales en Colombia frente a diferentes antimicrobianos.Para su aislamiento y tipificación se utilizaron técnicas microbiológicasconvencionales, pruebas bioquímicas, serológicas y pruebas de susceptibilidad a losantibióticos por difusión en agar. Los resultados revelaron una resistencia total hacia laestreptomicina, seguida de altas resistencias para tetraciclina y florfenicol, y una menorresistencia a productos como fosfomicina y cloramfenicol.

  18. Prueba de sensibilidad antimicrobiana de cepas de salmonella grupo D (móviles e inmóviles aisladas de ponedoras comerciales en Colombia

    Directory of Open Access Journals (Sweden)

    J. Mantilla

    2010-01-01

    Full Text Available Las infecciones originadas por bacterias del género Salmonella son una de las principales causas de pérdidas económicas en la industria avícola, se caracterizan generalmente por la presentación de cuadros diarreicos y septicémicos que llevan a las aves a una marcada disminución en la producción y a la muerte. En Colombia, debido al efecto negativo que produce Salmonella spp. en las aves, y con el objetivo de poder controlar la enfermedad, se utiliza una gran variedad de productos antimicrobianos, de los cuales no se posee suficiente información acerca de su comportamiento en cuanto a sensibilidad y resistencia frente a las cepas de Salmonella spp. de campo. El objetivo de este estudio fue determinar la respuesta de 20 cepas de Salmonellas grupo D (móviles e inmóviles aisladas de aves ponedoras comerciales en Colombia frente a diferentes antimicrobianos. Para su aislamiento y tipificación se utilizaron técnicas microbiológicas convencionales, pruebas bioquímicas, serológicas y pruebas de susceptibilidad a los antibióticos por difusión en agar. Los resultados revelaron una resistencia total hacia la estreptomicina, seguida de altas resistencias para tetraciclina y florfenicol, y una menor resistencia a productos como fosfomicina y cloramfenicol.

  19. Cloning, Codon Optimization, and Expression of Yersinia intermedia Phytase Gene in E. coli.

    Science.gov (United States)

    Mirzaei, Maryam; Saffar, Behnaz; Shareghi, Behzad

    2016-06-01

    Phytate is an anti-nutritional factor in plants, which catches the most phosphorus contents and some vital minerals. Therefore, Phytase is added mainly as an additive to the monogastric animals' foods to hydrolyze phytate and increase absorption of phosphorus. Y. intermedia phytase is a new phytase with special characteristics such as high specific activity, pH stability, and thermostability. Our aim was to clone, express, and characterizea codon optimized Y. intermedia phytase gene in E. coli . The Y. intermedia phytase gene was optimized according to the codon usage in E. coli . The sequence was synthesized and sub-cloned in pET-22b (+) vector and transformed into E. coli Bl21 (DE3). The protein was expressed in the presence of IPTG at a final concentration of 1 mM at 30°C. The purification of recombinant protein was performed by Ni 2+ affinity chromatography. Phytase activity and stability were determined in various pH and temperatures. The codon optimized Y. intermedia phytase gene was sub-cloned successfully.The expression was confirmed by SDS-PAGE and Western blot analysis. The recombinant enzyme (approximately 45 kDa) was purified. Specific activity of enzyme was 3849 (U.mg -1 ) with optimal pH 5 and optimal temperature of 55°C. Thermostability (80°C for 15 min) and pH stability (3-6) of the enzyme were 56 and more than 80%, respectively. The results of the expression and enzyme characterization revealed that the optimized Y. intermedia phytase gene has a good potential to be produced commercially andto be applied in animals' foodsindustry.

  20. Contribution of hly homologs to the hemolytic activity of Prevotella intermedia.

    Science.gov (United States)

    Suzuki, Naoko; Fukamachi, Haruka; Arimoto, Takafumi; Yamamoto, Matsuo; Igarashi, Takeshi

    2012-06-01

    Prevotella intermedia is a periodontal pathogen that requires iron for its growth. Although this organism has hemolytic activity, the precise nature of its hemolytic substances and their associated hemolytic actions are yet to be fully determined. In the present study, we identified and characterized several putative hly genes in P. intermedia ATCC25611 which appear to encode hemolysins. Six hly genes (hlyA, B, C, D, E, and hlyI) of P. intermedia were identified by comparing their nucleotide sequences to those of known hly genes of Bacteroides fragilis NCTC9343. The hlyA-E, and hlyI genes were overexpressed individually in the non-hemolytic Escherichia coli strain JW5181 and examined its contribution to the hemolytic activity on sheep blood agar plates. E. coli cells expressing the hlyA and hlyI genes exhibited hemolytic activity under anaerobic conditions. On the other hand, only E. coli cells stably expressing the hlyA gene were able to lyse the red blood cells when cultured under aerobic conditions. In addition, expression of the hlyA and hlyI genes was significantly upregulated in the presence of red blood cells. Furthermore, we found that the growth of P. intermedia was similar in an iron-limited medium supplemented with either red blood cells or heme. Taken together, our results indicate that the hlyA and hlyI genes of P. intermedia encode putative hemolysins that appear to be involved in the lysis of red blood cells, and suggest that these hemolysins might play important roles in the iron-dependent growth of this organism. Copyright © 2012 Elsevier Ltd. All rights reserved.

  1. Effect of azithromycin on Prevotella intermedia lipopolysaccharide-induced production of interleukin-6 in murine macrophages.

    Science.gov (United States)

    Choi, Eun-Young; Jin, Ji-Young; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

    2014-04-15

    Interleukin-6 (IL-6) is a key proinflammatory cytokine which plays a central role in the pathogenesis of periodontal disease. Host modulatory agents targeting at inhibiting IL-6, therefore, appear to be beneficial in slowing the progression of periodontal disease and potentially reducing destructive aspects of the host response. The present study was designed to investigate the effect of the macrolide antibiotic azithromycin on IL-6 generation in murine macrophages treated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in inflammatory periodontal disease, and its mechanisms of action. Azithromycin significantly suppressed IL-6 production as well as its mRNA expression in P. intermedia LPS-activated RAW264.7 cells. LPS-induced activation of JNK and p38 was not affected by azithromycin treatment. Azithromycin failed to prevent P. intermedia LPS from degrading IκB-α. Instead, azithromycin significantly diminished nuclear translocation and DNA binding activity of NF-κB p50 subunit induced with LPS. Azithromycin inhibited P. intermedia LPS-induced STAT1 and STAT3 phosphorylation. In addition, azithromycin up-regulated the mRNA level of SOCS1 in cells treated with LPS. In conclusion, azithromycin significantly attenuated P. intermedia LPS-induced production of IL-6 in murine macrophages via inhibition of NF-κB, STAT1 and STAT3 activation, which is possibly related to the activation of SOCS1 signaling. Further in vivo studies are required to better evaluate the potential of azithromycin in the treatment of periodontal disease. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. Effect of aflatoxin B1 on growth and enzymatic activity of a native strain of Bacillus sp Efecto de la aflatoxina B1 sobre el crecimiento y actividad proteolítica de una cepa nativa de Bacillus sp

    Directory of Open Access Journals (Sweden)

    Márquez Edna Judith

    2004-07-01

    Full Text Available The effect of different aflatoxin B1 (AFAB1 concentrations on alkaline protease growth and enzymatic activity was evaluated; a native strain of alkalophilic Bacillus sp cultivated in CSL (Corn Steep Liquor was used. It was found that the effect of AFAB1 on the strain inhibited its growth and enzymatic activity to 1 ppm, showing that the strain is highly sensible to AFAB1, meaning that medium obtained f rom Colombian corn contaminated with this mycotoxin cannot be easily used. Concentrations less than 0.1 ppm did not affect growth and enzymatic activity. Key words: Bacillus, aflatoxin, alkaline proteases.Se evaluó el efecto de diferentes concentraciones de aflatoxina B1 (AFAB1 sobre el crecimiento y actividad enzimática de proteasas alcalinas de una cepa nativa de Bacillus sp Alcalofílico cultivada en LAM (Licor Agotado de Maíz. Se encontró que la cepa inhibe su crecimiento y actividad enzimática a 1 ppm, lo que demuestra una alta sensibilidad de la cepa evaluada a la AFAB1 e imposibilita utilizar fácilmente medios obtenidos de maíz nacional contaminado con esta micotoxina. Las concentraciones inferiores a 0.1 ppm no tienen ningún efecto sobre el crecimiento y la actividad enzimática. Palabras clave: Bacillus, aflatoxina, proteasas alcalinas.

  3. OBTENCIÓN DE CEPAS Y PRODUCCIÓN DE INÓCULO DE CINCO ESPECIES DE HONGOS SILVESTRES COMESTIBLES DE ALTA MONTAÑA EN EL CENTRO DE MÉXICO

    Directory of Open Access Journals (Sweden)

    Yolanda Arana-Gabriel

    2014-01-01

    Full Text Available Se obtuvieron cepas de Psathyrella spadicea , Floccularia aff. luteovirens , Clitocybe squamulosa , Flammulina mexicana y Lyophyllum aff. shimeji , cuyos esporomas son recolectados y consumi - dos por habitantes del Nevado de Toluca, México. Las cepas se estudiaron in vitro en seis medios de cultivo (ACP: Agar croquetas de perro, AM: Agar maíz, PDA-PL: Agar papa dextrosa-pepto - na y levadura, EMA-PL: Agar extracto de malta-peptona y levadura, PDA y EMA a 18 °C y 25 °C. Las especies se desarrollaron satisfactoriamente a 18 °C , siendo F. mexicana y L . aff. shimeji las que tuvieron mayor velocidad de crecimiento y producción de biomasa. Flammulina mexicana solo se desarrolló a 18 °C, presentando mayor crecimiento y producción de biomasa en ACP. La cepa de L. aff. shimeji creció a 18 y 25 °C, la mayor velocidad de crecimiento se registró en medio PDA a 18 °C y la mayor producción de biomasa en EMA-PL a 18 °C. Considerando las mejores condiciones para el desarrollo de micelio in vitro, se produjo inóculo primario de F. mexicana y L . aff. shimeji en trigo y sorgo. La mayor densidad del micelio y porcentaje de grano cubierto se obtuvieron con el sustrato de trigo.

  4. Evaluación in vitro de las cepas de Enterococcus faecalis y Enterococcus faecium, como potenciales probióticos

    Directory of Open Access Journals (Sweden)

    Diana Alejandra Pico Veslin

    2016-06-01

    folato (Trimetropim sulfa, Betalactamicos (Ampicilina,Fenicol (Cloramfenicol y Florfenicol Fluroquinolonas (Ciprofloxacina del laboratorio Oxoid. Resultados: para el caso de las cepas de  E. faecium  94,1% muestran sensibildad frente a glucopeptidos (Vancomicina, 95% a inhibidores de  folato (Trimetropim sulfa,76% a fenicol(Clorafenicol, 100% a los  Betalactamicos (ampicilina, 19% a Fluoroquinolonas (Ciprofloxacina y un 95% a Fenicol ( Florfenicol. Se obtuvo  una  resistencia  de  5 %  para  Trimetropim sulfa, 19% para Clorafenicol, 52,3%  a la Ciprofloxacina, para E. faecalis se observó una sensibilidad del 100% frente a los antibióticos usados, excepto para la Ciprofloxacina un 50% de cepas sensibles. Conclusiones: Tres cepas de Enterococcus cumplen con los criterios de cepas seguras como potenciales probióticos. Posteriormente se determinará presencia y/ o ausencia del gen Van A mediante pruebas moleculares.

  5. Efecto de adición de iones hierro y zinc sobre la producción de etanol de dos cepas recombinantes de Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Carolina Peña Serna

    2010-01-01

    Full Text Available La producción de etanol por fermentación es influenciada por la presencia de iones metálicos como hierro y zinc dado que son cofactores de la enzima alcohol deshidrogenasa. El estudio de este efecto permitiría identificar el comportamiento de los microorganismos fermentadores en sustratos industriales que contienen altas concentraciones de este tipo de iones. Este trabajo evaluó la producción de biomasa, los azúcares residuales y la producción de etanol por fermentación de tres cepas de S. cerevisiae, CBS8066, recombinantes GG570-CIBI y GG570-CIBII, bajo el efecto de la adición de hierro a 0, 50 y 150 M, y zinc a 0 y 50 M. Las cepas presentaron inhibición en la producción de biomasa y etanol bajo efecto de iones de hierro y zinc, siendo dicha inhibición mayor al estar en presencia de zinc o alta concentración de hierro. GG570-CIBI mostró disminución en producción de biomasa de 4 g/L y una caída en producción de etanol de 40% en el tratamiento 150 M hierro-50 M zinc (con respecto al tratamiento basal. GG570-CIBII fue la menos afectada con inhibición en la producción de etanol inferior a 11% a las 20 h de fermentación. Adicionalmente, presentó la mayor producción de etanol cuando hubo adición de 150 M Fe con o sin adición de zinc, siendo dicha producción entre un 9 y 14% superior a la de las cepas CBS8066 y GG570-CIBI respectivamente, bajo las mismas condiciones. Posteriormente, GG570-CIBII será evaluada en sustratos industriales debido a su menor inhibición en la producción de etanol, permitiendo así obtener mejores rendimientos.

  6. Efectividad de cepas rizobianas nativas de sabana en Vigna unguiculata (L. Walp. cv. C4A-3

    Directory of Open Access Journals (Sweden)

    Juliana Mayz

    2010-07-01

    Full Text Available Título en inglés: Effectiveness of savannah native rhizobial strains in Vigna unguiculata (L. Walp. cv. C4A-3 Resumen Se estima que la población mundial se incrementará y demandará mayor cantidad de alimentos y uso de fertilizantes nitrogenados. En Venezuela, el frijol es altamente consumido y se cultiva en las sabanas orientales, cuyas características edáficas pueden afectar negativamente la población rizobiana. Estos planteamientos refuerzan la importancia de la evaluación de la flora rizobiana nativa, y enfatizan la necesidad de aumentar la explotación de la fijación biológica de nitrógeno. En este contexto, se evaluaron 6 cepas rizobianas en el cultivar C4A-3, aisladas, de frijol cv. Tejero Criollo y previamente catalogadas como efectivas (JV91, JV94 y JV101 e inefectivas (JV99, JV103, y JV104 en el cultivar TC9-6. El experimento se llevó a cabo en umbráculo por 45 días, donde además se incluyeron dos tratamientos control no inoculados. La suspensión de las cepas individualmente cultivadas se usó para inoculación. De acuerdo con la tipología de la nodulación (número de nódulos, peso total y por nódulo, tamaño y color, los valores de los parámetros de crecimiento (peso seco, altura y número de hojas del vástago y los estimados de la concentración de nitrógeno y nitrógeno total, las cepas JV91, JV99 y JV101, fueron las más efectivas en la fijación de nitrógeno. El nitrógeno total y la concentración de nitrógeno tuvieron una correlación significativa con peso seco, altura y número de hojas del vástago. Los resultados muestran la existencia de cepas efectivas en los suelos de sabana para este cultivar, y enfatizan la importancia de evaluar las cepas indígenas, antes de proceder a la inoculación con foráneas. Palabras clave: Rhizobium; frijol; fijación de nitrógeno; Venezuela. Abstract It is estimated that world-wide population will increase and demand higher amount of food and use of nitrogen

  7. Escherichia coli enteroagregativa en niños con diarrea de un hospital de Lima

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    Isabel Arias B

    2004-07-01

    Full Text Available Entre las Escherichia coli diarreogénicas la categoría E. coli enteroagregativa (ECEA es una de las más importantes y frecuentemente asociada a diarreas infantiles. El presente estudio se realizó con la finalidad de detectar los factores de virulencia que caracterizan a esta categoría patogénica mediante hibridación por colony blot usando sondas de ADN específicas. Se evaluaron 233 cepas aisladas en el laboratorio del Hospital de Emergencias Pediátricas durante los meses de diciembre 1998 y abril de 1999. Del total de muestras analizadas, se encontró que 17,16% de las cepas poseen el factor de virulencia característico de esta categoría. Los resultados obtenidos demuestran que un importante número de aislamientos de niños con diarrea presentan E. coli enteroagregativa.

  8. Towards onions and shallots (Allium cepa L.) resistant to beet armyworm (Spodoptera exigua Hübner) by transgenesis and conventional breeding

    NARCIS (Netherlands)

    Zheng, S.J.

    2000-01-01

    Onion ( Allium cepa L. group Common Onion) and shallot ( A. cepa L. group Aggregatum) are two subspecies of A. cepa . Both onion and shallot together with other Allium species like garlic ( A.

  9. Estrategia de verificación de calidad de las cepas de Escherichia coli conservadas en la Colección del Centro de Ingeniería Genética y Biotecnología

    Directory of Open Access Journals (Sweden)

    Iveldris Domínguez-Vázquez

    2010-04-01

    Full Text Available La colección de microorganismos de interés biotecnológico del Centro de Ingeniería Genética y Biotecnología incluye diferentes cepas de bacterias y levaduras utilizables en la manipulación de genes mediante el empleo de la ingeniería genética y para la producción de proteínas recombinantes por métodos biotecnológicos. La colección de hospederos de Escherichia coli centraliza todas las cepas que se utilizan en los proyectos de investigación-desarrollo de la institución. Este microorganismo constituye una de las herramientas biológicas imprescindibles para el trabajo en ingeniería genética y la investigación molecular. La fidelidad de los resultados experimentales depende en parte de la calidad de los bancos de estas cepas, que está determinada por las condiciones de almacenamiento y las estrategias de evaluación de los mismos. La complejidad del manejo de esta colección está influida en gran medida por el hecho de que en los medios de propagación pueden crecer un gran número de contaminantes ambientales de difícil identificación. Mientras más similar es el contaminante a la cepa de interés, más difícil es lograr bancos puros con una estabilidad confiable para la conservación. En este trabajo se describe cómo se garantiza el control de calidad de los bancos de Escherichia coli. Los métodos y estrategias de verificación que se describen han sido desarrollados en nuestro laboratorio.

  10. Genetic analyses of bolting in bulb onion (Allium cepa L.).

    Science.gov (United States)

    Baldwin, Samantha; Revanna, Roopashree; Pither-Joyce, Meeghan; Shaw, Martin; Wright, Kathryn; Thomson, Susan; Moya, Leire; Lee, Robyn; Macknight, Richard; McCallum, John

    2014-03-01

    We present the first evidence for a QTL conditioning an adaptive trait in bulb onion, and the first linkage and population genetics analyses of candidate genes involved in photoperiod and vernalization physiology. Economic production of bulb onion (Allium cepa L.) requires adaptation to photoperiod and temperature such that a bulb is formed in the first year and a flowering umbel in the second. 'Bolting', or premature flowering before bulb maturation, is an undesirable trait strongly selected against by breeders during adaptation of germplasm. To identify genome regions associated with adaptive traits we conducted linkage mapping and population genetic analyses of candidate genes, and QTL analysis of bolting using a low-density linkage map. We performed tagged amplicon sequencing of ten candidate genes, including the FT-like gene family, in eight diverse populations to identify polymorphisms and seek evidence of differentiation. Low nucleotide diversity and negative estimates of Tajima's D were observed for most genes, consistent with purifying selection. Significant population differentiation was observed only in AcFT2 and AcSOC1. Selective genotyping in a large 'Nasik Red × CUDH2150' F2 family revealed genome regions on chromosomes 1, 3 and 6 associated (LOD > 3) with bolting. Validation genotyping of two F2 families grown in two environments confirmed that a QTL on chromosome 1, which we designate AcBlt1, consistently conditions bolting susceptibility in this cross. The chromosome 3 region, which coincides with a functionally characterised acid invertase, was not associated with bolting in other environments, but showed significant association with bulb sucrose content in this and other mapping pedigrees. These putative QTL and candidate genes were placed on the onion map, enabling future comparative studies of adaptive traits.

  11. Incidencia y fármaco-resistencia de cepas de Staphylococcus spp aisladas de exudados conjuntivales Incidence and drug-resistance of Staphylococcus spp strains isolated from conjunctival swabs

    Directory of Open Access Journals (Sweden)

    Andrés Noya Cabaña

    2010-01-01

    Full Text Available OBJETIVO: Determinar la incidencia de cepas del género Staphylococcus aisladas de exudados conjuntivales y analizar su resistencia frente a diferentes antimicrobianos. MÉTODOS: Se realizó un estudio observacional descriptivo retrospectivo en el que se revisaron 3554 exudados conjuntivales a pacientes que acudieron en el período comprendido entre enero de 2002 a diciembre de 2003 y desde enero de 2005 hasta diciembre de 2007 al Instituto Cubano de Oftalmología «Ramón Pando Ferrer» por presentar un diagnóstico de infección ocular externa. RESULTADOS: Se aislaron 874 cepas de Staphylococcus aureus para un 47,5 % y 965 cepas de Staphylococcus spp. coagulasa negativa con prueba de patogenicidad positiva para un 52,4 %. En 69 de esos exudados los cultivos presentaron etiología mixta con dos bacterias diferentes, para el 3,7 %. Se determinaron los porcentajes de resistencia a las cepas aisladas pertenecientes al género Staphylococcus. CONCLUSIONES: Se encontró una alta incidencia de las especies del género Staphylococcus en las infecciones oculares, así como se pudo apreciar que la menor fármaco-resistencia para Staphylococcus aureus y Staphylococcus spp. coagulasa negativa correspondieron a los antimicrobianos ciprofloxacina y amikacina. La mayor fármaco-resistencia de las cepas de Staphylococcus aureus correspondió a eritromicina y tetraciclina y en Staphylococcus spp coagulasa negativa fue frente a la tetraciclina.OBJECTIVE: To determine the incidence of Staphylococcus strains isolated from conjunctival swaps and their resistance to several antimicrobial agents. METHODS: A retrospective, descriptive and observational study was performed to review 3554 conjunctival swabs from patients who went to "Ramón Pando Ferrer" Institute of Ophthalmology in the period from January 2002 to December 2009 due to a diagnosis of external ocular infection. RESULTS: From the total of conjunctival swabs, 874 Staphylococcus aureus strains (47.5 % and

  12. Mejoramiento de la floculación de cepas de levadura cervecera

    OpenAIRE

    Abreu, J.; González, J.; Rodríguez, Y.

    2008-01-01

    Las cepas de levadura originales usadas en las cervecerías cubanas: Saccharomyces uvarum U-57, Saccharomyces uvarum Budvar y Saccharomyces cerevisiae F-26, se probaron en columnas EBC a 10 ºC durante 7 días, atendiendo a sus propiedades tecnológicas fundamentales: crecimiento, floculación, sedimentación, atenuación, producción de alcohol y eficiencia fermentativa. La floculación de las cepas se estudió a través de aislamientos celulares y valoración morfológica. Los ...

  13. Virulencia de cepas de Listeria monocytogenes procedentes de cabras y sus derivados

    OpenAIRE

    Guicela Ramírez Bernal; Alma Virginia Lara Sagahón; Carlos Gerardo García Tovar; Efrén Díaz Aparicio; Víctor Rubén Tenorio Gutiérrez

    2014-01-01

    Se evaluó la virulencia de cepas de Listeria monocytogenes todas de serotipo 4b procedentes de cabras y sus derivados. Se observaron niveles de virulencia variables cuando se comparó la virulencia relativa (porcentaje de letalidad) en ratones BALB/c inoculados vía intravenosa o intragástrica y su capacidad para infectar macrófagos J774A.1, y células epiteliales Caco-2. Dos cepas obtenidas de alimento de cabras produjeron 100 % de letalidad por ambas vías de inoculación y no mostraron diferenc...

  14. Comparative genome analysis of Prevotella intermedia strain isolated from infected root canal reveals features related to pathogenicity and adaptation.

    Science.gov (United States)

    Ruan, Yunfeng; Shen, Lu; Zou, Yan; Qi, Zhengnan; Yin, Jun; Jiang, Jie; Guo, Liang; He, Lin; Chen, Zijiang; Tang, Zisheng; Qin, Shengying

    2015-02-25

    Many species of the genus Prevotella are pathogens that cause oral diseases. Prevotella intermedia is known to cause various oral disorders e.g. periodontal disease, periapical periodontitis and noma as well as colonize in the respiratory tract and be associated with cystic fibrosis and chronic bronchitis. It is of clinical significance to identify the main drive of its various adaptation and pathogenicity. In order to explore the intra-species genetic differences among strains of Prevotella intermedia of different niches, we isolated a strain Prevotella intermedia ZT from the infected root canal of a Chinese patient with periapical periodontitis and gained a draft genome sequence. We annotated the genome and compared it with the genomes of other taxa in the genus Prevotella. The raw data set, consisting of approximately 65X-coverage reads, was trimmed and assembled into contigs from which 2165 ORFs were predicted. The comparison of the Prevotella intermedia ZT genome sequence with the published genome sequence of Prevotella intermedia 17 and Prevotella intermedia ATCC25611 revealed that ~14% of the genes were strain-specific. The Preveotella intermedia strains share a set of conserved genes contributing to its adaptation and pathogenic and possess strain-specific genes especially those involved in adhesion and secreting bacteriocin. The Prevotella intermedia ZT shares similar gene content with other taxa of genus Prevotella. The genomes of the genus Prevotella is highly dynamic with relative conserved parts: on average, about half of the genes in one Prevotella genome were not included in another genome of the different Prevotella species. The degree of conservation varied with different pathways: the ability of amino acid biosynthesis varied greatly with species but the pathway of cell wall components biosynthesis were nearly constant. Phylogenetic tree shows that the taxa from different niches are scarcely distributed among clades. Prevotella intermedia ZT

  15. Bases ecofisiológicas de la producción de la cebolla (Allium cepa, L.): aportaciones para la mejora de las técnicas de cultivo en el Pla d'Urgell (Lleida)

    OpenAIRE

    Bosch Serra, Àngela D.

    1999-01-01

    El presente estudio se plantea a fin de optimizar las prácticas de cultivo de cebolla (Allium cepa L.) a la vez que se profundiza en las bases ecofisiológicas de su producción. La experimentación se ubica en la comarca del Pla d´Urgell (Lleida) donde constituye la principal hortícola . El cultivar más común, con siembra a partir de enero, es Valenciana de Grano (V. de Grano). Se recolecta en la segunda quincena de agosto y se destina a consumo en fresco aunque almacenándose para ser comercial...

  16. Detección de actividad pectolítica en el cultivo de la cepa GR4 de rhyzobíum meliloti

    Directory of Open Access Journals (Sweden)

    P. Martínez M.

    2010-07-01

    Full Text Available Se ensayaron varios métodos para la obtención y purificación parcial de pectinasas a partir de sobrenadante del cultivo de la cepa GR4 de Rhizobium meliloti. Se describe el método con el cual se obtuvo el sobrenadante en el que se logró detectar la presencia de actividad pectolítica. Empleando una muestra comercial de enzimas pécticas (Pectinex, Novo se estudió la estabilidad de la actividad enzimática durante el proceso de purificación parcial establecido; se observó una pérdida gradual de la actividad en función del tiempo de duración del proceso.

  17. Caracterización de germoplasma de cebollín (Allium cepa var. Aggregatum, en el municipio de Gibara, provincia de Holguín

    Directory of Open Access Journals (Sweden)

    Hubert Rodríguez García

    2010-01-01

    Full Text Available Se colectaron y estudiaron 18 accesiones de cebollín (Allium cepa var Aggregatum entre los productores del municipio Gibara se plantaron en las fincas de los productores de la CCS Mario Muñoz Gibara. El estudio se realizo en el período comprendido de enero del 2005 a diciembre del 2006, con el objetivo de realizar una caracterización del germoplasma para determinar las variedades existentes en del municipio. Entre los principales resultados obtenidos se identificaron dos variedades en el agroecosistema: Bola Roja de bulbos de color rojo violáceo, redondos globoso y pungencia fuerte y la variedad Amarillo Largo de bulbos amarillo naranja, fusiformes-oblongos y de pungencia media.

  18. Leptin potentiates Prevotella intermedia lipopolysaccharide-induced production of TNF-alpha in monocyte-derived macrophages.

    Science.gov (United States)

    Kim, Sung-Jo

    2010-06-01

    In addition to regulating body weight, leptin is also recognized for its role in the regulation of immune function and inflammation. The purpose of this study was to investigate the effect of leptin on Prevotella (P.) intermedia lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-alpha production in differentiated THP-1 cells, a human monocytic cell line. LPS from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. THP-1 cells were incubated in the medium supplemented with phorbol myristate acetate to induce differentiation into macrophage-like cells. The amount of TNF-alpha and interleukin-8 secreted into the culture medium was determined by enzyme-linked immunosorbent assay (ELISA). TNF-alpha and Ob-R mRNA expression levels were determined by semi-quantitative reverse transcription-polymerase chain reaction analysis. Leptin enhanced P. intermedia LPS-induced TNF-alpha production in a dose-dependent manner. Leptin modulated P. intermedia LPS-induced TNF-alpha expression predominantly at the transcriptional level. Effect of leptin on P. intermedia LPS-induced TNF-alpha production was not mediated by the leptin receptor. The ability of leptin to enhance P. intermedia LPS-induced TNF-alpha production may be important in the establishment of chronic lesion accompanied by osseous tissue destruction observed in inflammatory periodontal disease.

  19. Susceptibilidad "in vitro" de cepas de Cryptococcus a 5 drogas antifungicas "In vitro" susceptibility of Cryptococcus strains to 5 antifungal drugs

    Directory of Open Access Journals (Sweden)

    A. J. Bava

    1989-10-01

    Full Text Available Se estudió la susceptibilidad "in vitro" de 24 cepas de 3 especies del género Cryptococcus a 5 drogas antifúngicas (anfotericina B, 5 fluorocitosina, ketoconazol, itraconazol y miconazol. Las mismas se agruparon según su especie, variedad y origen de aislamiento. Para determinar la concentración inhibitoria mínima (C.I.M. de cada droga se empleó el método de dilución en agar con el medio básico nitrogenado para levaduras, adicionado de glucosa. Se obtuvo además la media geométrica de estos valores para cada grupo y se comparó cada uno de ellos. Los resultados obtenidos fueron homogéneos con la sola excepción de las cepas de Cryptococcus sp (no neoformans, en las cuales se detectaron elevados valores de C.I.M. para la 5 fluorocitosina.A comparative study of the "in vitro" susceptibility of 24 Cryptococcus strains to 5 antifungal drugs (amphotericin B, 5 fluorocytosine, miconazole, itraconazole and ketoconazole, was carried out. These strains were grouped according to species, varieties and isolation's origins. The minimum inhibitory concentration (M.I.C. was determinated by the agar dilution technique in yeast nitrogen base agar with dextrose. The mean geometrical of the M.I.C. values of each group was compared with the others. The results obtained were homogeneous with the only exception of the "non neoformans" strains, in which, higher M.I.C. to 5 fluorocytosine values were detected.

  20. Fábricas diagenéticas asociadas al paleokarst del techo de la Unidad Intermedia del Mioceno de la cuenca de Madrid

    Directory of Open Access Journals (Sweden)

    Calvo, J. P.

    1997-12-01

    Full Text Available Carbonates and evaporites from the top of the Miocene Intermediate Unit of the Madrid Basin have set as host-rock for early karstification phenomena. The development of these karstic processes imply an important change in the depositional evolution of the basin. In these materials, complex diagenetic fabrics formed as a result of shallow meteoric diagenetic processes have been recognized, being dedolomitization and calcitization of gypsums the main diagenetic processes that affected the top of the Miocene Intermediate Unit. The high variability of diagenetic fabrics is thought to be mainly influenced by the original composition of sediments, the activity of microorganisms and the chemistry of diagenetic waters.Los materiales carbonáticos y evaporíticos del techo de la Unidad Intermedia del Mioceno de la Cuenca de Madrid han actuado como soporte de fenómenos de karstificación temprana, cuyo desarrollo ha supuesto un cambio significativo en la evolución del relleno de la cuenca. Dentro de estos materiales se han reconocido carbonatos con fábricas diagenéticas complejas resultado de procesos de diagénesis meteórica superficial. Los procesos predominantes en la evolución diagenética del encajante de la paleokarstificación del techo de la Unidad Intermedia son la dedolomitización y la calcitización/pseudomorfización de yesos. Factores como la composición original del sedimento, la influencia de microorganismos y la hidroquímica de los fluidos diagenéticos han determinado la gran variabilidad de las fábricas diagenéticas reconocidas.

  1. Extramedullary hematopoiesis with spinal cord compression in a child with thalassemia intermedia.

    Science.gov (United States)

    Ileri, Talia; Azik, Fatih; Ertem, Mehmet; Uysal, Zumrut; Gozdasoglu, Sevgi

    2009-09-01

    Spinal cord compression due to extramedullary hematopoiesis is an extremely rare complication of thalassemia intermedia. No cases with this complication have been reported in the first decade of life, because masses of heterotropic marrow developed in patients as a result of continuous erythropoiesis. We report the 9-year-old patient suffering from thalassemia intermedia and presenting spinal cord compression. We also review the literature about treatment options, because there is no consensus about the optimal treatment of these patients. Our patient was successfully treated with radiation therapy followed by hydroxyurea. With this combination therapy, he had no recurrence during the 4-year follow-up period. Clinical awareness of this phenomenon with the early treatment is essential for optimizing the successful outcome.

  2. Interpretación de la prueba de tuberculina en niños y vacunados con BCG

    OpenAIRE

    Piñeiro Pérez, Roi

    2012-01-01

    Revisados 20 años de literatura médica, el debate sobre la conveniencia de la vacunación con Bacilo de Calmette-Guérin (BCG) continúa abierto. La OMS se muestra a favor de su utilización en países con endemia elevada de Tuberculosis (TB), pero no se ha esclarecido el beneficio ni las indicaciones de la inmunización en poblaciones con endemia intermedia o baja, y existen numerosos expertos que se muestran contrarios a su administración en cualquier caso. Existen muchas publicaciones sobre el ...

  3. Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia

    Directory of Open Access Journals (Sweden)

    Pavlović Snežana Đ.

    2009-01-01

    Full Text Available Gibberella intermedia (Kuhlmann Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg was isolated from seeds of St. John's wort, marshmallow, and coneflower, as well as from roots and stalks of marshmallow and roots of coneflower. These plants had symptoms of leaf chlorosis, malformation, withering and plant dwarfing and were collected from several localities in Serbia during five-year investigations of mycopopulations of the mentioned plants. The morphological characteristics of the pathogen were described.

  4. Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia

    OpenAIRE

    Pavlović Snežana Đ.; Stojšin Vera B.; Stojanović Saša D.; Starović Mira S.; Bagi Ferenc F.; Budakov Dragana B.

    2009-01-01

    Gibberella intermedia (Kuhlmann) Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg) was isolated from seeds of St. John's wort, marshmallow, and coneflower, as well as from roots and stalks of marshmallow and roots of coneflower. These plants had symptoms of leaf chlorosis, malformation, withering and plant dwarfing and were collected from several localities in Serbia during five-year investigations of mycopopulations of the mentioned plants. The morphological characterist...

  5. In Vitro Antibacterial and Antifungal Activity of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’

    OpenAIRE

    Blazekovic, Biljana; Stanic, Gordana; Pepeljnjak, Stjepan; Vladimir-Knezevic, Sanda

    2011-01-01

    This study aimed to evaluate the in vitro antibacterial and antifungal activities of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’, an indigenous Croatian cultivar of lavandin. For that purpose the activity of ethanolic extracts of flowers, inflorescence stalks and leaves against thirty one strains of bacteria, yeasts, dermatophytes and moulds were studied using both the agar well diffusion and broth dilution assays. Among the investigated extracts found to be effective against a broad ...

  6. Polarization of the interference field during reflection of electromagnetic waves from an intermedia boundary

    Science.gov (United States)

    Bulakhov, M. G.; Buyanov, Yu. I.; Yakubov, V. P.

    1996-10-01

    It has been shown that a full vector measurement of the total field allows one to uniquely distinguish the incident and reflected waves at each observation point without the use of a spatial difference based on an analysis of the polarization structure of the interference pattern which arises during reflection of electromagnetic waves from an intermedia boundary. We have investigated the stability of these procedures with respect to measurement noise by means of numerical modeling.

  7. DHA suppresses Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

    Science.gov (United States)

    Choi, Eun-Young; Jin, Ji-Young; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

    2014-04-14

    Several reports have indicated that dietary intake of DHA is associated with lower prevalence of periodontitis. In the present study, we investigated the effect of DHA on the production of proinflammatory mediators in murine macrophage-like RAW264.7 cells stimulated with lipopolysaccharide (LPS) isolated from Prevotella intermedia, a pathogen implicated in inflammatory periodontal disease, and its mechanisms of action. LPS was isolated from lyophilised P. intermedia ATCC 25,611 cells using the standard hot-phenol-water protocol. Culture supernatants were collected and assayed for NO, IL-1β and IL-6. Real-time PCR analysis was carried out to detect the expression of inducible NO synthase (iNOS), IL-1β, IL-6 and haeme oxygenase-1 (HO-1) mRNA. Immunoblot analysis was carried out to quantify the expression of iNOS and HO-1 protein and concentrations of signalling proteins. DNA-binding activities of NF-κB subunits were determined using an ELISA-based assay kit. DHA significantly attenuated the production of NO, IL-1β and IL-6 at both gene transcription and translation levels in P. intermedia LPS-activated RAW264.7 cells. DHA induced the expression of HO-1 in cells treated with P. intermedia LPS. Selective inhibition of HO-1 activity by tin protoporphyrin IX significantly mitigated the inhibitory effects of DHA on LPS-induced NO production. DHA significantly attenuated the phosphorylation of c-Jun N-terminal kinase induced by LPS. In addition, DHA suppressed the transcriptional activity of NF-κB by regulating the nuclear translocation and DNA-binding activity of NF-κB p50 subunit and inhibited the phosphorylation of signal transducer and activator of transcription 1. Further in vivo studies are needed to better evaluate the potential of DHA in humans as a therapeutic agent to treat periodontal disease.

  8. Analisis Strategi Pemasaran Internasional Pada Produk Revoluzio (Studi Pada PT Beon Intermedia)

    OpenAIRE

    Aisyah, Nugrahanti Khairani; Mawardi, M. Kholid

    2017-01-01

    PT Beon Intermedia is company engaged in the field of ICT (information and communication technology. Based on preliminary observations conducted by researchers in the field, Revoluzio is an innovative e-business to implement automation in digital marketing. This product is an innovative e-business the most and become the only one, both Indonesia and abroad, so the company should also establish appropriate strategy for the International market. The aim of this study was to describe the changes...

  9. CYTOGENETICS EFFECTS INDUCED BY NITRATE OF LEAD ON MITOTIC DIVISION AT ALLIUM CEPA L.

    OpenAIRE

    Silvica Padureanu

    2005-01-01

    The paper presents the influence of nitrate of lead upon the mitotic division of Allium cepa L. The treatment with nitrate of lead has determined the lessening of the mitotic index and the chromosomial mutations. Also nitrate of lead determined in little proportion cells autopoliploid. The experiment prowed that nitrate of lead, known as a polluting agent has a mutagenic potential on the plants.

  10. Clinical hypoglycemic effects of allium cepa (Red Onion) in Type 1 ...

    African Journals Online (AJOL)

    Background: Type 1 diabetes mellitus is an autoimmune disease caused by destruction of pancreatic islet beta cells and characterized by defect in insulin secretion. Objectives: The present study was carried out to investigate the hypoglycemic effects of Allium cepa in patients with type 1 diabetic patients. Results: In the ...

  11. Agrobacterium tumefaciens mediated transformation of Allium cepa L.: the production of transgenic onions and shallots

    NARCIS (Netherlands)

    Zheng, S.J.; Khrustaleva, L.I.; Henken, G.; Sofiari, E.; Jacobsen, E.; Kik, C.; Krens, F.A.

    2001-01-01

    This paper describes the development of a reliable transformation protocol for onion and shallot (Allium cepa L.) which can be used year-round. It is based on Agrobacterium tumefaciens as a vector, with three-week old callus, induced from mature zygotic embryos, as target tissue. For the development

  12. Screening for resistance to the beet armyworm (Spodoptera exigua) in Allium cepa and its wild relatives

    NARCIS (Netherlands)

    Zheng, S.J.; Henken, B.; Sofiari, E.; Wietsma, W.A.; Jacobsen, E.; Krens, F.A.; Kik, C.

    2000-01-01

    The beet armyworm (Spodoptera exigua Hübner) is the most important pest in tropical Allium cultivations. All shallot ( Allium cepa L. group Aggregatum) cultivars are susceptible to this pest. Therefore accessions from three wild Allium species, namely A. galanthum Kar. et Kir., A. fistulosum L. and

  13. Abnormal mitosis in root meristem cells of Allium cepa L. induced by ...

    African Journals Online (AJOL)

    This investigation was aimed to find mitotic abnormalities as cytological evidence induced by the dye in root tip cells of onion (Allium cepa L.) grown in different concentrations: 0.01, 0.05, 0.1, 0.5 and 1.0% (weight per volume) prepared in distilled water in separate treatment schedules for 24 and 48 h. Mitotic aberrations ...

  14. Penentuan Nilai Ld50 Ekstrak Etanol Bawang Bombay (Allium Cepa L.) Pada Mencit Jantan

    OpenAIRE

    Amalia, Nofria Rizki

    2017-01-01

    131501110 LD50 (Lethal Dose50) merupakan salah satu parameter yang dapat menentukan derajat toksisitas suatu bahan. Uji toksisitas ini mengevaluasi besarnya dosis yang dapat menimbulkan efek toksik dan kematian pada hewan uji. Bawang bombay (Allium cepa L.) memiliki khasiat sebagaiantibakteri, antioksidan, antimutagenik, dan antiinflamasi. Tujuandaripenelitianiniadalahuntuk menentukan nilai LD50dariekstraketanolbawang bombaypadamencitjantan. Penelitianinimerupakanpenelitianeksperimental...

  15. Morphology, morphogenesis, and phylogeny of an Anteholosticha intermedia (Ciliophora, Urostylida) population from the United States.

    Science.gov (United States)

    Chen, Lingyun; Wu, Weining; El-Serehy, Hamed A; Hu, Xiaozhong; Clamp, John C

    2018-04-30

    A distinct population of Anteholosticha intermedia was isolated from soil in the Great Smoky Mountains of North Carolina, USA, and its morphology, morphogenesis and molecular phylogeny investigated by microscopic observations of live and protargol-prepared specimens and analyses of the sequence of small subunit (SSU) rDNA. Our population closely resembles the populations from Austria and Korea. Members of the genus Anteholosticha have been regarded as ontogenetically diverse, which is confirmed by the present work. The most noteworthy ontogenetic feature of the American population of A. intermedia is that the oral primordium in the proter appears apokinetally at the posterior end of the undulating membranes anlage at the beginning of division and then dedifferentiates midway through morphogenesis. Molecular phylogenetic analyses demonstrate, with high support, that the American population of A. intermedia is clearly distinct from congeners and branches as part of a sister lineage to the Bakuella-Urostyla clade that belongs to the major clade comprising the order Urostylida. Copyright © 2018 Elsevier GmbH. All rights reserved.

  16. Effect of pollen irradiation on hybridization of wheat and Eltrigia intermedia and their embryo development

    International Nuclear Information System (INIS)

    Li Guiying; Wang Linqing; Shi Jinguo

    2005-01-01

    Spikes of Eltrigia intermedia were radiated with 5-100 Gy γ-ray during anthesis, and then their pollens were collected to pollinate to the common wheat 'J-11' and 'Chinese Spring'. The effects of pollen irradiation on the seed setting, embryo development, embryo culture and plantlet rate were studied. The results showed that low dose (5-9 Gy) of irradiation enhanced the seed setting for Chinese spring x E. intermedia, but no such effect for J-11xE. intermedia. Irradiation with all doses damaged embryo development, percentage of seeds with embryos; rate of immature hybrid embryos developing into plantlets decreases with the increased doses. Percentage of seeds with abnormal embryos increased significantly with the doses. 12.9%-14.5% of embryos could develop into plants in 30 Gy treatment, which seldom occur to wheat. Embryos in 50 Gy-100 Gy treatment were affected so serious that even none of them could develop into plants in vitro culture. It may be an effective approach to obtaining more mutants and enhancing induced mutation breeding by using combination of pollen irradiation, immature embryo rescue and anther culture of the resultant progenies. (authors)

  17. Cepas de Campylobacter jejuni resistentes a quinolonas aisladas de humanos, gallinas y pollos Quinolone resistant Campylobacter jejuni strains isolated from humans and from poultry

    Directory of Open Access Journals (Sweden)

    Rodolfo Notario

    2011-08-01

    Full Text Available Se compararon 8 aislamientos de Campylobacter jejuni provenientes de humanos con enfermedad diarreica aguda, con 23 aislamientos de cloaca de gallinas y pollos obtenidos de zonas próximas a la ciudad de Rosario, todos resistentes a la ciprofloxacina. Las muestras se sembraron en agar selectivo y se incubaron en microaerofilia a 42 °C. Las colonias se identificaron con el método tradicional. Los aislamientos se conservaron a -70 °C en caldo cerebro corazón con 17% v/v de glicerina. La clonalidad se determinó por RAPD-PCR, utilizando el primer 1254 (Stern NJ. Se interpretaron los aislamientos como clones distintos cuando diferían en una banda de amplificación. Se obtuvieron 5 clones diferentes. Los patrones I, II y V fueron aislados en criaderos industriales de pollos y en humanos (el II también en un establecimiento de gallinas ponedoras de huevos. En un gallinero familiar se obtuvo el patrón I. El patrón III sólo se obtuvo de humanos. El patrón IV se halló en uno de los criaderos pero no en humanos. Se pudo determinar que 93.5% de las cepas se aislaron tanto de animales como de humanos, por lo que se considera posible que la colonización de criaderos con cepas resistentes a los antimicrobianos pudiera ser el origen de la infección de humanos.Eight quinolone resistant Campylobacter jejuni strains isolated from humans with diarrheal disease were compared with 23 isolates from chicken and from laying hens. Samples were cultured on selective agar in microaerophilia, identified by conventional tests, and conserved in 17% glycerol at -70 °C. Clones were determined by RAPD-PCR employing the 1254 primer (Stern NJ. Five patterns were obtained. Patterns I, II, and V were found in both poultry and human isolates. Pattern I was obtained from poultry in a domestic henhouse. Pattern III was only obtained from humans whereas pattern IV was only obtained from poultry. A 95.3% of clones were found in both, humans and poultry. According to these

  18. Genome sequence of Prevotella intermedia SUNY aB G8-9K-3, a biofilm forming strain with drug-resistance.

    Science.gov (United States)

    Moon, Ji-Hoi; Kim, Minjung; Lee, Jae-Hyung

    Prevotella intermedia has long been known to be as the principal etiologic agent of periodontal diseases and associated with various systemic diseases. Previous studies showed that the intra-species difference exists in capacity of biofilm formation, antibiotic resistance, and serological reaction among P. intermedia strains. Here we report the genome sequence of P. intermedia SUNY aB G8-9K-3 (designated ATCC49046) that displays a relatively high antimicrobial resistant and biofilm-forming capacity. Genome sequencing information provides important clues in understanding the genetic bases of phenotypic differences among P. intermedia strains. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  19. Aislamiento y caracterización de cepas nativas de Lactobacillus spp. para su uso como probióticos en la industria láctea

    Directory of Open Access Journals (Sweden)

    Sylvia Vázquez

    2011-05-01

    Full Text Available La utilización de fermentos en la elaboración de productos lácteos es una práctica diaria a nivel industrial. En nuestro país los mismos son comprados a multinacionales extranjeras que se dedican a producir y comercializar fermentos; muchos de los cuales incorporan bacterias probióticas. Los probióticos pueden definirse como microorganismos que luego de ser consumidos en cantidades adecuadas, confieren algún efecto benéfico en el huésped. En el presente trabajo se realizó el aislamiento de una cepa de Lactobacillus de origen humano. Se identificó por tinción gram, prueba catalasa, crecimiento en anaerobiosis y aerobiosis y un test API 50 CH. Con el objetivo de probar propiedades probióticas de la cepa se llevaron a cabo estudios de resistencia al pH, tolerancia a sales biliares y se realizó un Modelo Gástrico in vitro. Los resultados permiten afirmar que estamos en presencia de una cepa nativa de Lactobacillus acidophilus caracterizada fenotípicamente con un 97% de confianza. Presentaría la habilidad de sobrevivir al pasaje a través del tubo digestivo ya que resistió la exposición a un pH similar al estomacal, pudo crecer en un medio con sales biliares y sobrevivió a la acción conjunta de la pepsina y una simulación de jugo gástrico; características que permiten clasificarla como posible cepa probiótica.Abstract  The use of starters to elaborate dairy products is a current practice in the industry. In our Country we import these starters from foreign companies dedicated to make and sale it, and most of them include probiotic bacterias. Probiotics can be defined as microorganisms that after be consumed in adequate amount, can give some advantageous effect to the host. In this study a strain of Lactobacillus was isolated from a human. The identification was done through gram stain, catalase test, aerobic and anaerobic growth, and an API 50 CH test. In order to prove the probiotic properties of the strain, studies of p

  20. Efectividad de la vacuna VA-MENGOC-BC® contra cepas heterólogas de meningococo B

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    Rolando Ochoa-Azze

    2016-08-01

    Full Text Available La efectividad de las vacunas de vesículas de membrana externa de Neisseria meningitidis serogrupo B ha sido cuestionada por algunos investigadores, limitándola a la cepa vacunal. VA-MENGOC-BC® es una vacuna antimeningocócica basada en dicha tecnología. Presentamos un metaanálisis de estudios realizados en diferentes contextos epidemiológicos, evaluando su efectividad contra cepas heterólogas de meningococo B en varios grupos de edades. Se demuestra que la vacuna es efectiva contra cepas homólogas, heterólogas y de diferentes complejos clonales.

  1. Sensibilidad antimicrobiana y caracterización de cepas de Streptococcus pyogenes aisladas de un brote de escarlatina

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    Pedraza-Avilés Alberto González

    2002-01-01

    Full Text Available Objetivo. Evaluar la actividad in vitro de 13 antibióticos contra 47 Streptococcus pyogenes grupo A (SGA. Determinar la presencia de genes que codifican para exotoxina pirogénica estreptocóccica A (SpeA y serotipos con base en proteína M. Material y métodos. Estudio transversal hecho en el Centro de Salud Dr. José Castro Villagrana sobre un brote de escarlatina en el Colegio Espíritu de América, entre diciembre de 1999 y enero de 2000. El número de niños estudiados fue 137. Se extrajeron porcentajes de sensibilidad. La concentración inhibitoria mínima (CIM se obtuvo por microdilución semiautomatizada. Se utilizó un secuenciador automatizado de DNA para el análisis de variación de secuencias en los genes que codifican para proteína M y SpeA. Resultados. Todas las cepas fueron sensibles a beta-lactámicos y clindamicina; 12.7% fueron resistentes a eritromicina. El serotipo M2 fue el más frecuente, 27 del total. Prácticamente todas las bacterias (96% con el gen SpeA tienen el gen que codifica para el serotipo M2. Conclusiones. Debido a la reciente reaparición de infecciones por SGA se sugiere realizar estudios tanto de sensibilidad a macrólidos y beta-lactámicos, como de epidemiología molecular.

  2. Valoración de desinfectantes mediante el método dilución-neutralización en cepas de Salmonella spp. aisladas en plantas de beneficio porcino

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    Andrea Paola Rodríguez Triviño

    2011-06-01

    Full Text Available La salmonelosis es una enfermedad muy importante, causante de problemas de salud pública ya que produce fiebres entéricas, gastroenteritis, bacteriemia, infecciones localizadas y estado de portador crónico, además se han informado como fuentes de contaminación los huevos, las carnes y la leche, tres de los alimentos más importantes consumidos por las personas. Por tal razón es importante determinar en la industria de los alimentos la susceptibilidad de Salmonella spp. a los desinfectantes, con el fin de contribuir a elaborar programas de prevención y control de este microorganismo, haciéndose necesaria la evaluación de su acción en cepas de Salmonella spp., en especial sobre aquellas transmitidas por los alimentos, además se debe medir la eficacia de los tipos de desinfectantes mediante el ensayo cuantitativo de suspensión para la evaluación de la actividad bactericida, en especial cuando a futuro se tenga como objetivo, evaluar el uso de desinfectantes en cepas de Salmonella spp. aisladas de plantas de beneficio porcino. Así que una vez se seleccionen las plantas de beneficio porcino objeto de la medición, en éstas se debe aislar una cantidad representativa de cepas de Salmonella spp., para someterlas a los diversos tipos de desinfectantes. El objetivo de la presente revisión es comparar la eficacia de tipos de desinfectantes que cuentan con un principio activo de amonio cuaternario y ácido peracético. Esperando que en futuros ensayos, por el método a nivel in vitro se puede comprobar la eficacia de los mismos.

  3. Valoración de desinfectantes mediante el método dilución-neutralización en cepas de Salmonella spp. aisladas en plantas de beneficio porcino

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    Jairo Humberto López Vargas

    2011-01-01

    Full Text Available La salmonelosis es una enfermedad muy importante, causante de problemas de salud pública ya que produce fiebres entéricas, gastroenteritis, bacteriemia, infecciones localizadas y estado de portador crónico, además se han informado como fuentes de contaminación los huevos, las carnes y la leche, tres de los alimentos más importantes consumidos por las personas. Por tal razón es importante determinar en la industria de los alimentos la susceptibilidad de Salmonella spp. a los desinfectantes, con el fin de contribuir a elaborar programas de prevención y control de este microorganismo, haciéndose necesaria la evaluación de su acción en cepas de Salmonella spp., en especial sobre aquellas transmitidas por los alimentos, además se debe medir la eficacia de los tipos de desinfectantes mediante el ensayo cuantitativo de suspensión para la evaluación de la actividad bactericida, en especial cuando a futuro se tenga como objetivo, evaluar el uso de desinfectantes en cepas de Salmonella spp. aisladas de plantas de beneficio porcino. Así que una vez se seleccionen las plantas de beneficio porcino objeto de la medición, en éstas se debe aislar una cantidad representativa de cepas de Salmonella spp., para someterlas a los diversos tipos de desinfectantes. El objetivo de la presente revisión es comparar la eficacia de tipos de desinfectantes que cuentan con un principio activo de amonio cuaternario y ácido peracético. Esperando que en futuros ensayos, por el método a nivel in vitro se puede comprobar la eficacia de los mismos.

  4. Determinación de la frecuencia de Vibrio parahaemolyticus y otros vibriones halofilicos en alimentos preparados con productos marinos frescos y procesados

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    Amabilia Vilcapoma

    2014-06-01

    Full Text Available El trabajo se realizó durante las estaciones de verano a otoño de 1988, sobre un total de 122 muestras, correspondiendo 82 a pescado congelado y 40 a cebiches, en su mayoría de tipo "mixto"; todos procedentes de la ciudad de Lima. El procesamiento de las muestras y el aislamiento de los vibrio se efectuaron siguiendo la metodología recomendada por la FDA (1985, con algunas modificaciones. La identificación de las cepas se realizó en base a caracteristicas de colonia, morfología celular y pruebas bioquímicas diferenciales. Se obtuvieron 57 cepas presuntivas de vibriones halofílicos, de las cuales 42 procedieron de pescado congelado y 15 de "cebiches"; se identificaron 5 cepas de Vibrio parahaemoIyticus (8.8% y 14 cepas de Vibrio alginolyticus (24.6% 38 cepas de Vibrio no fueron especificados, catalogándose como Vlbrio sp. (66.6%.

  5. Infección por Helicobacter pylori en la Ciudad de La Habana, Cuba.Prevalencia de las cepas cagA positivas

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    Beatriz Gutiérrez

    2005-12-01

    Full Text Available Existe una gran falta de información acerca de la infección por Helicobacter pylori en los países de la región del Caribe. Nuestros objetivos en este estudio fueron determinar la prevalencia, la resistencia a los antibióticos y los factores de virulencia de la bacteria. La medida de la prevalencia de la infección por H. pylori se determinó en un grupo de pacientes a los que se les practicó una endoscopia en tres centros hospitalarios de La Ciudad de La Habana, lo que nos permitió evaluar la resistencia a la claritromicina y la presencia de cagA + en las cepas obtenidas. De las endoscopias realizadas se obtuvieron 117 biopsias gástricas, procedentes de tres centros hospitalarios de La Ciudad de La Habana, Cuba: Instituto de Oncología, Instituto de Gastroenterología y el Hospital Calixto García. Las biopsias fueron mantenidas a –70 ºC para posterior cultivo en tres medios diferentes (dos selectivos y uno no selectivo y su posterior incubación por 7 días a 37 ºC en una atmósfera de microaerofilia. La presencia de H. pylori fue identificada por la presencia de diferentes enzimas (oxidasa, catalasa, ureasa. Se realizó la extracción del DNA y la PCR, donde se utilizó el primer H2761676 y se amplificó con 397 fragmentos del gen cagA. La susceptibilidad a la claritromicina fue medida por el método de difusión en gel. Diagnóstico endoscópico: (1 cáncer gástrico; (19 úlcera duodenal; (8 úlcera gástrica; (89 dispepsias no ulcerosas, incluyendo (62 gastritis; (9 hernia hiatal; (2 reflujo biliar; (1 pólipo gástrico; (15 panendoscopias normales. Del total de 117 biopsias realizadas, 83 fueron positivas a la infección por H.pylori (70,9% . De las 35 cepas a las que se les realizó presencia de cagA+ resultaron positivas 31 (88,5%. Solo el 3% de las cepas fueron resistentes a la claritromicina. La prevalencia de la infección por H. pylori en la población sintomática de La Ciudad de La Habana es la misma que la reportada en

  6. Anti-inflammatory effect of extract and fractions from the leaves of Byrsonima intermedia A. Juss. in rats.

    Science.gov (United States)

    Moreira, Lucimara Q; Vilela, Fabiana C; Orlandi, Lidiane; Dias, Danielle F; Santos, Ana Laura A; da Silva, Marcelo A; Paiva, Renato; Alves-da-Silva, Geraldo; Giusti-Paiva, Alexandre

    2011-11-18

    Byrsonima intermedia is commonly used for its antiseptic, antimicrobial, and anti-inflammatory properties in the treatment of diarrhea and dysentery in Brazilian folk medicine. The purpose of this study was to examine the anti-inflammatory activity of the aqueous extract and fractions of Byrsonima intermedia leaves. Rats with carrageenan-induced paw edema and fibrovascular tissue growth, which was induced by subcutaneous implantation of a cotton pellet, were used as acute and chronic animal models of inflammation to investigate the anti-inflammatory effects of the aqueous extract and the individual ethyl acetate (EtOAc) and aqueous fractions of Byrsonima intermedia and catechin. High-performance liquid chromatography (HPLC) was used to determine the fingerprint chromatogram of the aqueous extract and fractions of Byrsonima intermedia. The crude aqueous extract at test doses of 30-300 mg/kg p.o. clearly demonstrated anti-inflammatory effects by reducing carrageenan-induced paw edema, as did the ethyl acetate (100mg/kg) and aqueous fractions (30-100mg/kg). In the chronic inflammation rat animal model with fibrovascular tissue growth, the aqueous extract of Byrsonima intermedia (BiAE) at doses of 30-300 mg/kg and the individual EtOAc and aqueous fractions at doses of 30-100mg/kg and catechin significantly reduced the formation of granulomatous tissue. The presence of catechin and phenolic compounds in the extract and fractions of Byrsonima intermedia was confirmed using HPLC. BiAE and the individual EtOAc and aqueous fractions of Byrsonima intermedia exhibited chronic and acute anti-inflammatory efficacy in rats, which supports previous claims of its use in traditional medicine. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  7. Effect of quercetin on the production of nitric oxide in murine macrophages stimulated with lipopolysaccharide from Prevotella intermedia.

    Science.gov (United States)

    Cho, Yun-Jung; Kim, Sung-Jo

    2013-08-01

    Nitric oxide (NO) is a short-lived bioactive molecule that is known to play an important role in the pathogenesis of periodontal disease. In the current study, we investigated the effect of the flavonoid quercetin on the production of NO in murine macrophages activated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen related to inflammatory periodontal disease, and tried to elucidate the underlying mechanisms of action. LPS was isolated from P. intermedia ATCC 25611 cells by the standard hot phenol-water method. The concentration of NO in cell culture supernatants was determined by measuring the accumulation of nitrite. Inducible NO synthase (iNOS) and heme oxygenase-1 (HO-1) protein expression, phosphorylation of c-Jun N-terminal kinase (JNK) and p38, inhibitory κB (IκB)-α degradation, and signal transducer and activator of transcription 1 (STAT1) phosphorylation were analyzed via immunoblotting. Quercetin significantly attenuated iNOS-derived NO production in RAW246.7 cells activated by P. intermedia LPS. In addition, quercetin induced HO-1 protein expression in cells activated with P. intermedia LPS. Tin protoporphyrin IX (SnPP), a competitive inhibitor of HO-1, abolished the inhibitory effect of quercetin on LPS-induced NO production. Quercetin did not affect the phosphorylation of JNK and p38 induced by P. intermedia LPS. The degradation of IκB-α induced by P. intermedia LPS was inhibited when the cells were treated with quercetin. Quercetin also inhibited LPS-induced STAT1 signaling. Quercetin significantly inhibits iNOS-derived NO production in murine macrophages activated by P. intermedia LPS via anti-inflammatory HO-1 induction and inhibition of the nuclear factor-κB and STAT1 signaling pathways. Our study suggests that quercetin may contribute to the modulation of host-destructive responses mediated by NO and appears to have potential as a novel therapeutic agent for treating inflammatory periodontal disease.

  8. Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood.

    Science.gov (United States)

    Weinkopff, Tiffany; de Oliveira, Camila I; de Carvalho, Augusto M; Hauyon-La Torre, Yazmin; Muniz, Aline C; Miranda, Jose Carlos; Barral, Aldina; Tacchini-Cottier, Fabienne

    2014-01-01

    During a blood meal, Lutzomyia intermedia sand flies transmit Leishmania braziliensis, a parasite causing tegumentary leishmaniasis. In experimental leishmaniasis, pre-exposure to saliva of most blood-feeding sand flies results in parasite establishment in absence of any skin damages in mice challenged with dermotropic Leishmania species together with saliva. In contrast, pre-immunization with Lu. intermedia salivary gland sonicate (SGS) results in enhanced skin inflammatory exacerbation upon co-inoculation of Lu. intermedia SGS and L. braziliensis. These data highlight potential unique features of both L. braziliensis and Lu. intermedia. In this study, we investigated the genes modulated by Lu. intermedia SGS immunization to understand their potential impact on the subsequent cutaneous immune response following inoculation of both SGS and L. braziliensis. The cellular recruitment and global gene expression profile was analyzed in mice repeatedly inoculated or not with Lu. intermedia. Microarray gene analysis revealed the upregulation of a distinct set of IFN-inducible genes, an immune signature not seen to the same extent in control animals. Of note this INF-inducible gene set was not induced in SGS pre-immunized mice subsequently co-inoculated with SGS and L. braziliensis. These data suggest the parasite prevented the upregulation of this Lu. intermedia saliva-related immune signature. The presence of these IFN-inducible genes was further analyzed in peripheral blood mononuclear cells (PBMCs) sampled from uninfected human individuals living in a L. braziliensis-endemic region of Brazil thus regularly exposed to Lu. intermedia bites. PBMCs were cultured in presence or absence of Lu. intermedia SGS. Using qRT-PCR we established that the IFN-inducible genes induced in the skin of SGS pre-immunized mice, were also upregulated by SGS in PBMCs from human individuals regularly exposed to Lu. intermedia bites, but not in PBMCs of control subjects. These data demonstrate

  9. Etiología bacteriana de la infección urinaria y susceptibilidad antimicrobiana en cepas de Escherichia coli Bacterial etiology of urinary sepsis and antimicrobial susceptibility in strains from Escherichia coli

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    Leonor Díaz Rigau

    2006-09-01

    Full Text Available La infección del tracto urinario constituye un problema de salud frecuente. Se realizó un estudio descriptivo-retrospectivo con el objetivo de conocer el comportamiento y la frecuencia de los aislamientos bacterianos más frecuentes encontrados en muestras de orina de pacientes con diagnóstico presuntivo de infección urinaria. Los pacientes tenían edades comprendidas entre 0 y 15 años y fueron hospitalizados en el Hospital General Docente «Aleida Fernández Chardiet», en Güines, entre el 2003 y el 2004. El mayor número de aislamientos correspondió a Escherichia coli, Klebsiella sp., Proteus vulgaris, entre otros. Las cepas de Escherichia coli presentaron niveles de sensibilidad superiores al 90 % para los antibióticos ciprofloxacina, norfloxacina y amikacina, entre 85 % y 90 % para las 3 cefalosporinas en estudio y aminoglucósidos (gentamicina y kanamicina. El 64,2 % y 57,7 % de las cepas presentaron resistencia a la ampicilina y al trimetoprin-sulfametozaxol, respectivamente.

  10. Genotypic variation in the sulfur assimilation and metabolism of onion (Allium cepa L.) I. Plant composition and transcript accumulation

    KAUST Repository

    McCallum, John A.; Thomas, Ludivine; Shaw, Martin L.; Pither-Joyce, Meeghan D.; Leung, Susanna; Cumming, Mathew; McManus, Michael T.

    2011-01-01

    Organosulfur compounds are major sinks for assimilated sulfate in onion (Allium cepa L.) and accumulation varies widely due to plant genotype and sulfur nutrition. In order to better characterise sulfur metabolism phenotypes and identify potential

  11. Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts.

    Science.gov (United States)

    Aydin, Kubra; Ekinci, Fatma Yesim; Korachi, May

    2015-04-01

    The presence of certain oral pathogens at implant sites can hinder the osseointegration process. However, it is unclear how and by what microorganisms it happens. This study investigated whether the presence of oral pathogens of Porphyromonas gingivalis and Prevotella intermedia individually, play a role in the failure of bone formation by determining the expression profiles of Transforming Growth Factor Beta (TGF-β/Bone Morphogenic Protein (BMP) and Toll-Like Receptor (TLR) pathways in challenged osteoblasts. Cell viability of P. gingivalis and P. intermedia challenged osteoblasts were determined by WST assay. Changes in osteoblast morphology and inhibition of mineralization were observed by Scanning Electron Microscopy (SEM) and Von Kossa staining, respectively. Expression of TGF-β and TLR pathway genes on challenged cells were identified by RT profiler array. Both P. gingivalis and P. intermedia challenges resulted in reduced viability and mineralization of osteoblasts. Viability was reduced to 56.8% (P. gingivalis) and 52.75% (P. intermedia) at 1000 multiplicity. Amongst 48 genes examined, expressions of BMPER, SMAD1, IL8 and NFRKB were found to be highly upregulated by both bacterial challenges (Fold Change > 4). P. gingivalis and P. intermedia could play a role in implant failure by changing the expression profiles of genes related to bone formation and resorption.

  12. Evaluación serológica de una proteína recombinante a partir de una cepa aislada del virus de la fiebre amarilla en el Perú: un estudio piloto

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    Carlos Yábar V

    2003-10-01

    Full Text Available Objetivo: Evaluar serológicamente una proteína recombinante de 66 kDa (Er66 de una cepa del virus de la fiebre amarilla aislada en el Perú usando anticuerpos inmunoreactivos. Material y métodos: La proteína Er66 fue expresada in vitro en la bacteria Escherichia coli y enfrentada a títulos de 1/100, 1/50 y 1/25 de anticuerpos monoclonales, y a sueros con anticuerpos IgM e IgG inmunoreactivos contra el virus de la fiebre amarilla mediante ensayos de Western blot (WB. Asimismo, se evaluaron proteínas totales de las cepas de referencia del virus dengue (DEN y fiebre amarilla como controles de antigenicidad. Resultados: La proteína recombinante Er66 presentó antigenicidad contra títulos de 1/50 y 1/25 de anticuerpo monoclonal (MAB8701; sin embargo, no se observó inmunoreactividad en sueros positivos para fiebre amarilla y dengue. Los ensayos con extractos crudos de la cepa de fiebre amarilla Asibi 17D (PTFA revelaron que la antigenicidad de las proteínas virales comprendidas entre 60 y 80 kDa fue afectada negativamente a temperaturas desnaturalizantes de 100°C. En cambio, tratamientos con ß-mercaptoetanol sin calor generó un aumento de la antigenicidad de dichas proteínas. Siguiendo este mismo principio, la proteína Er66 fue sometida a tratamientos desnaturalizantes con ß-mercaptoetanol sin incluir calor y fue enfrentada nuevamente a los sueros reactivos. El resultado final reveló que la proteína Er66 generó inmunoreactividad frente a sueros con altos títulos de anticuerpos IgM e IgG para fiebre amarilla mientras que en otras muestras se observó una débil señal. Conclusiones: Los datos obtenidos en este trabajo demuestran que la Er66 pudo ser reconocida por anticuerpos IgM e IgG específicos para fiebre amarilla presentes en sueros de pacientes infectados. Los datos presentados en este artículo sugieren el uso de proteínas recombinantes como posibles candidatas de valor diagnóstico para la FA.

  13. Evaluation of neutralization patterns of the five unique Argentine equine arteritis virus field strains reported Evaluación de los patrones de neutralización de las únicas cinco cepas argentinas descritas de arteritis viral equina

    Directory of Open Access Journals (Sweden)

    M. G. Echeverría

    2010-02-01

    proteína GP5, especialmente en la región V1, afectan el fenotipo neutralizante, sin tener en cuenta variaciones aminoacídicas de otras proteínas virales. En este estudio evaluamos los fenotipos neutralizantes de las 5 únicas cepas de arteritis viral equina aisladas en Argentina y los comparamos con los de la cepa de referencia EAV-UCD por virus neutralización cruzada y análisis de secuencias aminoacídicas de las proteínas M y GP5. Las cepas argentinas presentaron un patrón de neutralización similar cuando se utilizaron sueros positivos del banco de sueros, mientras que fueron neutralizadas en menor medida por los sueros policlonales de referencia anti-AVE. A excepción de la cepa LP01, las cepas argentinas tienen casi las mismas sustituciones aminoacídicas en la primera región variable V1 de la proteína GP5, específicamente en los sitios neutralizantes B y C, pero difieren en gran medida respecto de la cepa de referencia EAV-UCD. Las diferencias encontradas en los aislamientos LP02/R, LP02/C, LP02/P y LT-LP-ARG no se reflejaron en variaciones en el fenotipo neutralizante.

  14. Evaluation of Trichoderma spp. and Clonostachys spp. Strains to Control Fusarium circinatum in Pinus radiata Seedlings Evaluación de Cepas de Trichoderma spp. y Clonostachys spp. para Controlar Fusarium circinatum en Plántulas de Pinus radiata

    Directory of Open Access Journals (Sweden)

    Priscila Moraga-Suazo

    2011-09-01

    Full Text Available The fungus Fusarium circinatum Nirenberg & O’Donnell causes pine pitch canker, an important disease for conifers worldwide. F. circinatum was first detected in Chile in 2001 and to date is present in nurseries and clonal hedges from Libertador General Bernardo O’Higgins Region to Los Rios Region. The purpose of this study was to evaluate the potential of Trichoderma spp. and Clonostachys spp. strains to control F. circinatum in Pinus radiata D. Don seedlings in the absence of other effective control methods. Eighty-one Trichoderma spp. and Clonostachys spp. strains were evaluated through in vitro assays to determine their ability to act as antagonists of F. circinatum and 21 strains were tested for their ability to reduce post-emergence mortality and increase P. radiata survival under greenhouse conditions. During in vitro experiments, 15 strains of Trichoderma inhibited mycelial growth of the pathogen by more than 60% and one strain of Clonostachys showed parasitism of F. circinatum hyphae. Greenhouse experiments showed no control of the disease when the antagonists were added to substrate after the pathogen. However, when the antagonists were added before the pathogen, four strains (Clonostachys UDC-32 and UDC-222 and Trichoderma UDC-23 and UDC-408 reduced post-emergence mortality between 80 and 100%. Among these strains, only Clonostachys UDC-222 significantly increased the survival of P. radiata seedlings. These results showed that Clonostachys UDC-222 has the potential to be used as a biocontrol agent against F. circinatum in the production of P. radiata plants.Fusarium circinatum Nirenberg & O’Donnell es el hongo que causa el cancro resinoso del pino, una enfermedad de importancia mundial en coníferas. En Chile, F. cicirnatum fue detectado por primera vez el año 2001 y a la fecha se encuentra presente en algunos viveros y huertos clonales desde la Región del Libertador General Bernardo O’Higgins hasta la Región de Los R

  15. Viabilidad del uso de suero de quesería como base del medio de cultivo de la cepa nativa probiótica Lactobacillus paracasei HA9-2

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    Sylvia Vázquez

    2011-05-01

    Full Text Available El suero de quesería constituye un importante residuo de la industria láctea en Uruguay, por lo cual es importante encontrar alternativas viables para su uso. En este trabajo se evaluó el suero de queso como base para el desarrollo de un medio de cultivo de la cepa probiótica nativa Lactobacillus paracasei HA9-2. Se ensayaron diferentes concentraciones de carbohidratos y se evaluaron los efectos del agregado de sales como sulfato de magnesio y manganeso, una fuente de nitrógeno y del prebiótico comercial goma de acacia. A efectos comparativos se realizaron cultivos en MRS, medio específi co para bacterias lácticas. Los resultados obtenidos indican que el suero de queso con una concentración de carbohidratos de 70 g/l es un medio óptimo para el desarrollo de esta cepa. Tanto el agregado de sales como de fuente de nitrógeno y prebiótico producen mejoras importantes en la biomasa. Pero en el balance de costos realizado, al comparar la formulación del medio con el incremento de la biomasa producida, se concluye que el suero de queso como único componente es el óptimo para el cultivo de la cepa probiótica HA9-2.Asimismo, se probó el efecto prebiótico de la goma de acacia sobre esta cepa.AbstractThe cheese whey is an important residue of the dairy industry in Uruguay, so it is necessary to fi nd new viable alternatives for its use. We evaluated the cheese whey as a base for the development of a probiotic native Lactobacillus paracasei HA9-2 strain culture media. Several carbohydrate concentrations, salt effects as magnesium sulfate and manganese sulfate, a nitrogen source and a commercial prebiotic addition as acacia rubber were assayed. Comparative assays in lactic bacteria commercial media known as MRS were made. The results indicate that the cheese whey with 70 g/l of carbohydrates is an ideal media for the strain development. The salt addition as well as the nitrogen source and commercial prebiotic addition improve and increase

  16. Expresión diferencial de proteínas en Leishmania (Viannia panamensis asociadas con mecanismos de resistencia a antimoniato de meglumina

    Directory of Open Access Journals (Sweden)

    Ronald Guillermo Peláez

    2012-05-01

    Full Text Available Introducción. Los mecanismos de resistencia al antimonio pentavalente conocidos hasta el momento,se han descrito ampliamente en cepas del subgénero Leishmania, pero poco se sabe sobre lasproteínas involucradas en los mecanismos de resistencia presentes en cepas del subgénero Viannia,como Leishmania panamensis. Objetivo. Identificar proteínas diferencialmente expresadas entre las cepas de L. panamensis(UA140, sensible y resistente al antimonio pentavalente, y analizar el posible papel de estas proteínasen mecanismos de resistencia. Materiales y métodos. Las proteínas de las cepas, sensible y resistente al antimonio pentavalente, secompararon usando electroforesis bidimensional. Las proteínas con aumento de la expresión fueronaisladas e identificadas por espectrometría de masas mediante MALDI-TOF/TOF (Matrix AssistedLaser Desorption Ionization/Time of Flight. La expresión del ARNm de cinco de estas proteínas secuantificó mediante PCR en tiempo real. Resultados. Los geles bidimensionales de las cepas sensible y resistente detectaron 532±39 y 541±43manchas proteicas. Se encontraron 10 manchas con aumento de la expresión en la cepa resistente,identificadas como proteínas de choque térmico (Hsp60 mitocondrial, Hsp70 mitocondrial y citosólica,isomerasa de disulfuro, proteasa de cisteína, enolasa, factor de elongación 5-α, la subunidad 5-α delproteasoma y dos proteínas hipotéticas nombradas como Sp(2 y Sp(25. Conclusión. Este es el primer estudio llevado a cabo con una cepa resistente al antimonio pentavalenteen L. panamensis, en el cual se han identificado proteínas que están relacionadas con el mecanismode resistencia del parásito frente al medicamento, abriendo el camino para futuros estudios de estasproteínas como blancos terapéuticos.   doi: http://dx.doi.org/10.7705/biomedica.v32i3.392

  17. Estudio microbiológico de ajo(Allium sativum L. y cebolla (Allium cepa L. deshidratados Microbiological study of garlic (Allium sativum L. and onion (Allium cepaL. dehydrated

    Directory of Open Access Journals (Sweden)

    S.R. Fuselli

    2004-09-01

    Full Text Available En este trabajo se efectuó un estudio microbiológico durante la deshidratación y el almacenamiento de ajo (Allium sativum L. y de cebolla (Allium cepa L.. Al ajo se le efectuó un proceso de escaldado y a la cebolla un salmuereado previo a la deshidratación. En las materias primas los recuentos promedio expresados en UFC/g fueron: bacterias aerobias mesófilas entre 1,2 x 102y 1,6 x 103, mohos y levaduras entre 60 y 1,6 x 103, Lactobacillus spp. y Leuconostocmesenteroidessubesp. mesenteroides entre 10 y 50.Se identificaron: Penicillium spp., Monilia spp. Lactobacillus brevis,Leuconostoc mesenteroides subesp. mesenteroides y levaduras en ajo; Mucor spp., Penicillium spp., Monilia spp., Lactobacillus brevis y levaduras en dos tipos de cebolla. Leuconostoc mesenteroides subesp. mesenteroides sólo se detectó en un tipo de cebolla. En ajos deshidratados y durante el almacenamiento se hallaron Penicillium spp., Monilia spp., Lactobacillus brevis y levaduras mientras que los mismos con escaldado no presentaron desarrollo. Mucor spp., Penicillium spp., Monilia spp. y Lactobacillus brevis se identificaron en los dos tipos de cebolla deshidratadas. Con la incorporación del salmuereado la microflora se redujo significativamente hallándose solamente Penicillium spp. La utilización de barreras adicionales de control microbiano tales como escaldado y salmuereado produce un aporte importante a la estabilidad microbiológica de los productos.A microbiological study during the process and the storage of garlic (Allium sativum L. and onion (Allium cepa L. dehydrated, with the additional barriers of blanching or brine immersion, was made. In all raw materials the average counts of aerobic mesophilic bacteria expressed in CFU/g ranged from 1.2 x 102to 1.6 x 103, molds and yeasts from 60 to 1.6 x 103, Lactobacillus spp. and Leuconostocmesenteroides subsp. mesenteroides between 10 and 50. Microorganisms identified were Penicillium spp., Monilia spp

  18. Pathogenicity of facultative and obligate anaerobic bacteria in monoculture and combined with either Prevotella intermedia or Prevotella nigrescens.

    Science.gov (United States)

    Siqueira, J F; Magalhães, F A; Lima, K C; de Uzeda, M

    1998-12-01

    The pathogenicity of obligate and facultative anaerobic bacteria commonly found in endodontic infections was tested using a mouse model. The capacity of inducing abscesses was evaluated seven days after subcutaneous injection of the bacteria in pure culture and in combinations with either Prevotella intermedia or Prevotella nigrescens. Nine of the fifteen bacterial strains tested were pathogenic in pure culture. No statistically significant differences were detected between these strains in pure culture and in mixtures with either P. intermedia or P. nigrescens. Synergism between the bacterial strains was only apparent when associating Porphyromonas endodontalis with P. intermedia or P. nigrescens. Histopathological examination of tissue sections from induced abscesses revealed an acute inflammatory reaction, dominated by polymorphonuclear leukocytes. Sections from the control group using sterile medium showed no evidence of inflammatory reaction.

  19. Prevalência de sorotipos e resistência antimicrobiana de cepas invasivas do Streptococcus pneumoniae

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    Mantese Orlando C.

    2003-01-01

    Full Text Available OBJETIVO: Avaliar o perfil de sorotipos e a susceptibilidade aos antimicrobianos de cepas de Streptococcus pneumoniae obtidas em espécimes clínicos de pacientes com doença invasiva, bem como suas implicações na formulação de vacinas pneumocócicas. MÉTODOS: Cepas de pneumococo isoladas no Laboratório de Análises Clínicas do Hospital de Clínicas da Universidade Federal de Uberlândia a partir de amostras clínicas de pacientes com doença invasiva foram identificadas e enviadas ao Instituto Adolfo Lutz em São Paulo para confirmação da identificação, sorotipagem e determinação da susceptibilidade aos antimicrobianos. RESULTADOS: De abril de 1999 a março de 2003, foram isoladas 148 cepas invasivas de pneumococo, sendo 84 (56,7% provenientes de pacientes do sexo masculino. A idade variou de um dia a 88,83 anos, com média de 21,33+25,82 anos e mediana de 4,42 anos. Os diagnósticos clínicos mais comuns foram pneumonia (91 casos; 61,4%, meningite (32 casos; 21,6% e bacteremia sem foco evidente (15 casos; 10,1%. As principais fontes de recuperação foram sangue (76 amostras; 51,3%, líquido pleural (39; 26,3% e liquor (30; 20,2%. No total, foram identificados 23 diferentes sorotipos entre 143 amostras testadas, sendo os mais comuns os seguintes: 14, 3, 1, 5, 6A, 6B e 18C. Dentre 30 (20,2% cepas oxacilina-resistentes, 23 (15,5% confirmaram a resistência à penicilina (12,8% com nível intermediário e 2,7%, com nível pleno, que esteve restrita aos sorotipos 14, 23F, 19A e 6B, predominando em indivíduos com até dois anos de idade (p = 0,0008. Foi detectada susceptibilidade diminuída ao cotrimoxazol (63,4%, à eritromicina (8,3%, à clindamicina (8,7% e à ofloxacina (0,8%. A resistência à cefotaxima foi detectada em três das 30 cepas testadas (2% das 148, todas elas com resistência confirmada à penicilina. Não foi observada resistência a cloranfenicol, rifampicina ou vancomicina. CONCLUSÕES: A resistência

  20. Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia.

    Science.gov (United States)

    Wan, Yongqing; Mao, Mingzhu; Wan, Dongli; Yang, Qi; Yang, Feiyun; Mandlaa; Li, Guojing; Wang, Ruigang

    2018-02-09

    WRKY transcription factors, one of the largest families of transcriptional regulators in plants, play important roles in plant development and various stress responses. The WRKYs of Caragana intermedia are still not well characterized, although many WRKYs have been identified in various plant species. We identified 53 CiWRKY genes from C. intermedia transcriptome data, 28 of which exhibited complete open reading frames (ORFs). These CiWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and zinc finger motifs. Conserved domain analysis showed that the CiWRKY proteins contain a highly conserved WRKYGQK motif and two variant motifs (WRKYGKK and WKKYEEK). The subcellular localization of CiWRKY26 and CiWRKY28-1 indicated that these two proteins localized exclusively to nuclei, supporting their role as transcription factors. The expression patterns of the 28 CiWRKYs with complete ORFs were examined through quantitative real-time PCR (qRT-PCR) in various tissues and under different abiotic stresses (drought, cold, salt, high-pH and abscisic acid (ABA)). The results showed that each CiWRKY responded to at least one stress treatment. Furthermore, overexpression of CiWRKY75-1 and CiWRKY40-4 in Arabidopsis thaliana suppressed the drought stress tolerance of the plants and delayed leaf senescence, respectively. Fifty-three CiWRKY genes from the C. intermedia transcriptome were identified and divided into three groups via phylogenetic analysis. The expression patterns of the 28 CiWRKYs under different abiotic stresses suggested that each CiWRKY responded to at least one stress treatment. Overexpression of CiWRKY75-1 and CiWRKY40-4 suppressed the drought stress tolerance of Arabidopsis and delayed leaf senescence, respectively. These results provide a basis for the molecular mechanism through which CiWRKYs mediate stress tolerance.

  1. Treatment with hydroxyurea in thalassemia intermedia with paravertebral pseudotumors of extramedullary hematopoiesis.

    Science.gov (United States)

    Cario, H; Wegener, M; Debatin, K-M; Kohne, E

    2002-08-01

    Excessive ineffective erythropoiesis in thalassemia intermedia may cause paravertebral pseudotumors of extramedullary hematopoiesis. Due to the proximity to the spinal canal, these paravertebral masses carry the risk of severe neurological damage. Treatment strategies include hypertransfusion, radiotherapy, and laminectomy. Hydroxyurea, stimulating fetal hemoglobin synthesis, may represent an alternative therapeutic approach. We report on a 26-year-old patient suffering from thalassemia intermedia with progressive anemia symptoms and presenting multiple intrathoracic paravertebral pseudotumors of extramedullary hematopoiesis. Hypertransfusion therapy and splenectomy were followed by regular transfusion (baseline hemoglobin 10 g/dl) and chelation with desferrioxamine. With this treatment, clinical symptoms disappeared, paravertebral hematopoietic masses did not progress, but severe hemosiderosis developed within a few years. Hydroxyurea therapy was initiated to increase the efficacy of erythropoiesis, thereby reducing the required transfusion volume but suppressing concomitantly further expansion of extramedullary hematopoiesis, and finally leading to a reduction of transfusional iron load. Treatment was started with 4 mg/kg per day and stepwise increased to 12.5 mg/kg per day. The fetal hemoglobin concentration increased from 4.5 to 5.5 g/dl after 1 year and to 9.9 g/dl after 2 years of treatment. The yearly transfusion volume was halved during the 1st year of treatment. At present, after 26 months of treatment, the patient has been transfusion-independent for 10 months. Serum ferritin levels decreased from 2844 to 1335 ng/ml. Size and shape of paravertebral hematopoietic pseudotumors remained stable. No side effects of hydroxyurea have been observed. In thalassemia intermedia patients with extramedullary hematopoiesis, hydroxyurea may lead to independence from regular transfusion therapy without further expansion of ectopic hematopoietic tissue.

  2. Transcriptome Changes Associated with Anaerobic Growth in Yersinia intermedia (ATCC29909)

    Science.gov (United States)

    Kiley, Patricia J.; Glasner, Jeremy D.; Perna, Nicole T.

    2013-01-01

    Background The yersiniae (Enterobacteriaceae) occupy a variety of niches, including some in human and flea hosts. Metabolic adaptations of the yersiniae, which contribute to their success in these specialized environments, remain largely unknown. We report results of an investigation of the transcriptome under aerobic and anaerobic conditions for Y. intermedia, a non-pathogenic member of the genus that has been used as a research surrogate for Y. pestis. Y. intermedia shares characteristics of pathogenic yersiniae, but is not known to cause disease in humans. Oxygen restriction is an important environmental stimulus experienced by many bacteria during their life-cycles and greatly influences their survival in specific environments. How oxygen availability affects physiology in the yersiniae is of importance in their life cycles but has not been extensively characterized. Methodology/Principal Findings Tiled oligonucleotide arrays based on a draft genome sequence of Y. intermedia were used in transcript profiling experiments to identify genes that change expression in response to oxygen availability during growth in minimal media with glucose. The expression of more than 400 genes, constituting about 10% of the genome, was significantly altered due to oxygen-limitation in early log phase under these conditions. Broad functional categorization indicated that, in addition to genes involved in central metabolism, genes involved in adaptation to stress and genes likely involved with host interactions were affected by oxygen-availability. Notable among these, were genes encoding functions for motility, chemotaxis and biosynthesis of cobalamin, which were up-regulated and those for iron/heme utilization, methionine metabolism and urease, which were down-regulated. Conclusions/Significance This is the first transcriptome analysis of a non-pathogenic Yersinia spp. and one of few elucidating the global response to oxygen limitation for any of the yersiniae. Thus this study

  3. Prevotella intermedia infection causing acute and complicated aortitis-A case report.

    Science.gov (United States)

    Boersma, C; Kampschreur, L M; Buter, H; Doorenbos, B M; Klinkert, P; Koning, G G

    2017-01-01

    Aortitis is a general term that refers to all conditions involving an inflammation of the aortic wall. This case report describes the surgical approach of a patient with infectious and symptomatic aortitis caused by the rare vector Prevotella intermedia. A 44-year old male patient was admitted with fever and general discomfort after a period of sore throat in a non-teaching hospital. After two weeks he developed acute abdominal and back pain accompanied by sweating and elevated infection parameters. Computed tomography angiography revealed atherosclerotic changes of the infrarenal aorta with a locally contained rupture of the aorta alongside peri-aortal signs of inflammation (and aortitis aspects). An urgent aortic reconstruction was performed according to Nevelsteen. The blood cultures turned out positive for Prevotella intermedia. Postoperatively the patient received antibiotics for six weeks. The patient recovered uneventful from this infection and surgical procedure. A complicated and acute aortitis is a rare but potentially life-threatening disease. The aetiology can be ordered into two main groups; inflammatory and infectious. Diagnosis is based upon symptoms, biochemical values, microbiological results and imaging modalities. Treatment depends on aetiology and should be discussed in an experienced multidisciplinary setting. Infectious aortitis should be treated with antibiotics for at least six weeks with close monitoring of the patient's clinic and biochemical values, even after surgery. Prevotella intermedia is a rare causative agent for aortitis. Acute aortitis is a challenging clinical entity which should be managed in an equipped medical center by an experienced multidisciplinary team. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  4. Suscetibilidade de biomphalaria glabrata, B. straminea e B. tenagophila a diferentes cepas de schistosoma mansoni

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    Luiz Candido de Souza Dias

    1987-08-01

    Full Text Available Em condições experimentais foi estudada a suscetibilidade de Biomphalaria glabrata, B. straminea e B. tenagophila a quatro linhagens humanas (MAP, PTH, UPH, e OuH e duas de roedores silvestres (PTR e VPR do Schistosoma mansoni. Grupos de 50 moluscos foram expostos individualmente a 10 miracídios e observados durante 70 dias. Avaliou-se a suscetibilidade dos moluscos ao parasito por meio da % de animais com esporocistos, % de moluscos que eliminavam cercárias e mortalidade conjunta dos animais expostos e infectados. Exemplares de B. glabrata mineira infectaram-se com cepa simpátrica (MAP e com 5 alopátricas do Estado de São Paulo (PTH, VPH, OuH, PTR e VPR. B. glabrata paulista mostrou altas taxas de infecção com as cepas MAP, VPR e OuH do trematódeo. Quatro % dos exemplares B. straminea de São Paulo eliminavam cercárias de cepas simpátricas; com cepa mineira apenas 4% apresentaram esporocistos na vigência de 20 miracídios por molusco; as menores taxas de mortalidade foram registradas com essa espécie de molusco, não sendo maior do que 20%. B. tenagophila paulista foi suscetível apenas às linhagens simpáticas sendo 6% a maior taxa de moluscos que eliminaram cercárias. Os resultados indicam que os movimentos populacionais humanos dentro do território paulista e para fora dele são importantes na disseminação da esquistossomose mansônica.

  5. Effects of polyamines and polyamine biosynthetic inhibitors on mitotic activity of Allium cepa root tips.

    Science.gov (United States)

    Unal, Meral; Palavan-Unsal, Narcin; Tufekci, M A

    2008-03-01

    The genotoxic and cytotoxic effects of exogenous polyamines (PAs), putrescine (Put), spermidine (Spd), spermine (Spm) and PA biosynthetic inhibitors, alpha-difluoromethylornithine (DFMO), cyclohexilamine (CHA), methylglioxal bis-(guanylhydrazone) (MGBG) were investigated in the root meristems of Allium cepa L. The reduction of mitotic index and the induction of chromosomal aberrations such as bridges, stickiness, c-mitotic anaphases, micronuclei, endoredupliction by PAs and PA biosynthetic inhibitors were observed and these were used as evidence of genotoxicity and cytotoxicity.

  6. Strip Tillage and Early-Season Broadleaf Weed Control in Seeded Onion (Allium cepa)

    OpenAIRE

    Sarah Gegner-Kazmierczak; Harlene Hatterman-Valenti

    2016-01-01

    Field experiments were conducted in 2007 and 2008 near Oakes, North Dakota (ND), USA, to evaluate if strip tillage could be incorporated into a production system of seeded onion (Allium cepa) to eliminate the standard use of a barley (Hordeum vulgare) companion crop with conventional, full width tillage, yet support common early-season weed control programs. A split-factor design was used with tillage (conventional and strip tillage) as the main plot and herbicide treatments (bromoxynil, DCPA...

  7. Effect of different exposed lights on quercetin and quercetin glucoside content in onion (Allium cepa L.)

    OpenAIRE

    Ko, Eun Young; Nile, Shivraj Hariram; Sharma, Kavita; Li, Guan Hao; Park, Se Won

    2014-01-01

    Quercetin and quercetin glucosides are the major flavonols present in onion (Allium cepa L.) and are predominantly present as quercetin, quercetin-3,4′-diglucoside and quercetin-4′-glucoside. Effect of different light wavelengths on onion after harvest and storage, with fluorescent, blue, red and ultra violet light influenced the quercetin and quercetin glucosides profile. In a peeled onion, all the light treatments elevated quercetin content in bulb. Among them, particularly fluorescent ligh...

  8. Population-Genomic Insights into Variation in Prevotella intermedia and Prevotella nigrescens Isolates and Its Association with Periodontal Disease

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    Yifei Zhang

    2017-09-01

    Full Text Available High-throughput sequencing has helped to reveal the close relationship between Prevotella and periodontal disease, but the roles of subspecies diversity and genomic variation within this genus in periodontal diseases still need to be investigated. We performed a comparative genome analysis of 48 Prevotella intermedia and Prevotella nigrescens isolates that from the same cohort of subjects to identify the main drivers of their pathogenicity and adaptation to different environments. The comparisons were done between two species and between disease and health based on pooled sequences. The results showed that both P. intermedia and P. nigrescens have highly dynamic genomes and can take up various exogenous factors through horizontal gene transfer. The major differences between disease-derived and health-derived samples of P. intermedia and P. nigrescens were factors related to genome modification and recombination, indicating that the Prevotella isolates from disease sites may be more capable of genomic reconstruction. We also identified genetic elements specific to each sample, and found that disease groups had more unique virulence factors related to capsule and lipopolysaccharide synthesis, secretion systems, proteinases, and toxins, suggesting that strains from disease sites may have more specific virulence, particularly for P. intermedia. The differentially represented pathways between samples from disease and health were related to energy metabolism, carbohydrate and lipid metabolism, and amino acid metabolism, consistent with data from the whole subgingival microbiome in periodontal disease and health. Disease-derived samples had gained or lost several metabolic genes compared to healthy-derived samples, which could be linked with the difference in virulence performance between diseased and healthy sample groups. Our findings suggest that P. intermedia and P. nigrescens may serve as “crucial substances” in subgingival plaque, which may

  9. Population-Genomic Insights into Variation in Prevotella intermedia and Prevotella nigrescens Isolates and Its Association with Periodontal Disease.

    Science.gov (United States)

    Zhang, Yifei; Zhen, Min; Zhan, Yalin; Song, Yeqing; Zhang, Qian; Wang, Jinfeng

    2017-01-01

    High-throughput sequencing has helped to reveal the close relationship between Prevotella and periodontal disease, but the roles of subspecies diversity and genomic variation within this genus in periodontal diseases still need to be investigated. We performed a comparative genome analysis of 48 Prevotella intermedia and Prevotella nigrescens isolates that from the same cohort of subjects to identify the main drivers of their pathogenicity and adaptation to different environments. The comparisons were done between two species and between disease and health based on pooled sequences. The results showed that both P. intermedia and P. nigrescens have highly dynamic genomes and can take up various exogenous factors through horizontal gene transfer. The major differences between disease-derived and health-derived samples of P. intermedia and P. nigrescens were factors related to genome modification and recombination, indicating that the Prevotella isolates from disease sites may be more capable of genomic reconstruction. We also identified genetic elements specific to each sample, and found that disease groups had more unique virulence factors related to capsule and lipopolysaccharide synthesis, secretion systems, proteinases, and toxins, suggesting that strains from disease sites may have more specific virulence, particularly for P. intermedia . The differentially represented pathways between samples from disease and health were related to energy metabolism, carbohydrate and lipid metabolism, and amino acid metabolism, consistent with data from the whole subgingival microbiome in periodontal disease and health. Disease-derived samples had gained or lost several metabolic genes compared to healthy-derived samples, which could be linked with the difference in virulence performance between diseased and healthy sample groups. Our findings suggest that P. intermedia and P. nigrescens may serve as "crucial substances" in subgingival plaque, which may reflect changes in

  10. Comparison of right and left side heart functions in patients with thalassemia major, patients with thalassemia intermedia, and control group.

    Science.gov (United States)

    Noori, Noormohammad; Mohamadi, Mehdi; Keshavarz, Kambiz; Alavi, Seyed Mostafa; Mahjoubifard, Maziar; Mirmesdagh, Yalda

    2013-01-01

    Heart disease is the main cause of mortality and morbidity in patients with beta thalassemia, rendering its early diagnosis vital. We studied and compared echocardiographic findings in patients with beta thalassemia major, patients with beta thalassemia intermedia, and a control group. Eighty asymptomatic patients with thalassemia major and 22 asymptomatic cases with thalassemia intermedia (8-25 years old) were selected from those referred to Ali Asghar Hospital (Zahedan-Iran) between June 2008 and June 2009. Additionally, 80 healthy individuals within the same age and sex groups were used as controls. All the individuals underwent echocardiography, the data of which were analyzed with the Student t-test. The mean value of the pre-ejection period/ejection time ratio of the left ventricle during systole, the diameter of the posterior wall of the left ventricle during diastole, the left and right isovolumic relaxation times, and the right myocardial performance index in the patients with beta thalassemia major and intermedia increased significantly compared to those of the controls, but the other parameters were similar between the two patient groups. The mean values of the left and right pre-ejection periods, left ventricular end systolic dimension, and left isovolumic contraction time in the patients with thalassemia intermedia increased significantly compared to those of the controls. In the left side, myocardial performance index, left ventricular mass index, isovolumic contraction time, and deceleration time exhibited significant changes between the patients with thalassemia major and those with thalassemia intermedia, whereas all the echocardiographic parameters of the right side were similar between these two groups. The results showed that the systolic and diastolic functions of the right and left sides of the heart would be impaired in patients with thalassemia major and thalassemia intermedia. Consequently, serial echocardiography is suggested in

  11. Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective salivary protein against Leishmania braziliensis infection.

    Science.gov (United States)

    de Moura, Tatiana R; Oliveira, Fabiano; Carneiro, Marcia W; Miranda, José Carlos; Clarêncio, Jorge; Barral-Netto, Manoel; Brodskyn, Cláudia; Barral, Aldina; Ribeiro, José M C; Valenzuela, Jesus G; de Oliveira, Camila I

    2013-01-01

    Leishmania parasites are transmitted in the presence of sand fly saliva. Together with the parasite, the sand fly injects salivary components that change the environment at the feeding site. Mice immunized with Phlebotomus papatasi salivary gland (SG) homogenate are protected against Leishmania major infection, while immunity to Lutzomyia intermedia SG homogenate exacerbated experimental Leishmania braziliensis infection. In humans, antibodies to Lu. intermedia saliva are associated with risk of acquiring L. braziliensis infection. Despite these important findings, there is no information regarding the repertoire of Lu. intermedia salivary proteins. A cDNA library from the Salivary Glands (SGs) of wild-caught Lu. intermedia was constructed, sequenced, and complemented by a proteomic approach based on 1D SDS PAGE and mass/mass spectrometry to validate the transcripts present in this cDNA library. We identified the most abundant transcripts and proteins reported in other sand fly species as well as novel proteins such as neurotoxin-like proteins, peptides with ML domain, and three small peptides found so far only in this sand fly species. DNA plasmids coding for ten selected transcripts were constructed and used to immunize BALB/c mice to study their immunogenicity. Plasmid Linb-11--coding for a 4.5-kDa protein--induced a cellular immune response and conferred protection against L. braziliensis infection. This protection correlated with a decreased parasite load and an increased frequency of IFN-γ-producing cells. We identified the most abundant and novel proteins present in the SGs of Lu. intermedia, a vector of cutaneous leishmaniasis in the Americas. We also show for the first time that immunity to a single salivary protein from Lu. intermedia can protect against cutaneous leishmaniasis caused by L. braziliensis.

  12. Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.

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    Natalia M Tavares

    Full Text Available BACKGROUND: Leishmania transmission occurs in the presence of insect saliva. Immunity to Phlebotomus papatasi or Lutzomyia longipalpis saliva or salivary components confers protection against an infection by Leishmania in the presence of the homologous saliva. However, immunization with Lutzomyia intermedia saliva did not protect mice against Leishmania braziliensis plus Lu. intermedia saliva. In the present study, we have studied whether the immunization with Lu. longipalpis saliva or a DNA plasmid coding for LJM19 salivary protein would be protective against L. braziliensis infection in the presence of Lu. intermedia saliva, the natural vector for L. braziliensis. METHODOLOGY/PRINCIPAL FINDINGS: Immunization with Lu. longipalpis saliva or with LJM19 DNA plasmid induced a Delayed-Type Hypersensitivity (DTH response against Lu. longipalpis as well as against a Lu. intermedia saliva challenge. Immunized and unimmunized control hamsters were then intradermally infected in the ears with L. braziliensis in the presence of Lu. longipalpis or Lu. intermedia saliva. Animals immunized with Lu. longipalpis saliva exhibited smaller lesion sizes as well as reduced disease burdens both at lesion site and in the draining lymph nodes. These alterations were associated with a significant decrease in the expression levels of IL-10 and TGF-β. Animals immunized with LJM19 DNA plasmid presented similar findings in protection and immune response and additionally increased IFN-γ expression. CONCLUSIONS/SIGNIFICANCE: Immunization with Lu. longipalpis saliva or with a DNA plasmid coding LJM19 salivary protein induced protection in hamsters challenged with L. braziliensis plus Lu. intermedia saliva. These findings point out an important role of immune response against saliva components, suggesting the possibility to develop a vaccine using a single component of Lu. longipalpis saliva to generate protection against different species of Leishmania, even those

  13. Los límites de la ciudad intermedia : conectividad e innovación

    OpenAIRE

    Basurto Alvarez, Rodolfo

    2014-01-01

    Este trabajo vincula conectividad e innovación para fijar los alcances de la segunda modernidad de una ciudad intermedia (Hermosillo, Sonora, México), estableciendo sus restricciones para ascender como nodo de comunicación sur-norte de la ciudad global. Se evalúa la diversidad incipiente del ecosistema urbano mediante el referente hipotético de la existencia de flujos comunicacionales de una sola dirección (norte-sur) en el funcionamiento de las empresas multinacionales. ...

  14. Evolución cenozoica de la Depresión Intermedia (Cuenca-Guadalajara)

    OpenAIRE

    Torres, T.; Zapata, J. L.

    1987-01-01

    En este trabajo se analiza la evolución Cenozoica de la Depresión Intermedia, habiéndose determinado la existencia de dos ciclos paleógenos y cuatro neógenos. En todos ellos la sedimentación es continental y de carácter fluvial y lacustre-palustre. Si se exceptúa el Primer Ciclo Paleógeno, que tuvo su área fuente en una zona al sur de la cuenca en la que predominaban rocas paleozoicas, los cinco restantes las tuvieron en el Sistema Ibérico, donde predominan rocas mesozoicas

  15. Evolución cenozoica de la Depresión Intermedia (Cuenca-Guadalajara)

    OpenAIRE

    Torres Pérez-Hidalgo, Trinidad José

    1987-01-01

    En este trabajo se analiza la evolución Cenozoica de la Depresión Intermedia, habiéndose determinado la existencia de dos ciclos paleógenos y cuatro neógenos. En todos ellos la sedimentación es continental y de carácter fluvial y lacustre-palustre. Si se exceptúa el Primer Ciclo Paleógeno, que tuvo su área fuente en una zona al sur de la cuenca en la que predominaban rocas paleozoicas, los cinco restantes las tuvieron en el Sistema Ibérico, donde predominan rocas mesozoicas....

  16. Double heterozygocity for hemoglobin C and beta thalassemia dominant: A rare case of thalassemia intermedia

    Directory of Open Access Journals (Sweden)

    Alexandra Agapidou

    2018-01-01

    Full Text Available Beta thalassemia dominant results from mutations in the β globin chain gene resulting in the production of elongated, highly unstable beta globin chains. Several such mutations have been described and in a heterozygous state they may confer a phenotype more severe than that of β thalassemia trait and lead to a clinical syndrome of thalassemia intermedia and its associated complications such as extramedullary hemopoiesis, bone disease, endocrinopathies and iron overload even in the absence of transfusion. In this report we present a case of double heterozygocity for HbC and β thalassemia dominant leading to a series of complications that were treated successfully once the correct diagnosis was made.

  17. Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing.

    Science.gov (United States)

    Monteiro, Carolina Cunha; Villegas, Luis Eduardo Martinez; Campolina, Thais Bonifácio; Pires, Ana Clara Machado Araújo; Miranda, Jose Carlos; Pimenta, Paulo Filemon Paolucci; Secundino, Nagila Francinete Costa

    2016-08-31

    Parasites of the genus Leishmania cause a broad spectrum of diseases, collectively known as leishmaniasis, in humans worldwide. American cutaneous leishmaniasis is a neglected disease transmitted by sand fly vectors including Lutzomyia intermedia, a proven vector. The female sand fly can acquire or deliver Leishmania spp. parasites while feeding on a blood meal, which is required for nutrition, egg development and survival. The microbiota composition and abundance varies by food source, life stages and physiological conditions. The sand fly microbiota can affect parasite life-cycle in the vector. We performed a metagenomic analysis for microbiota composition and abundance in Lu. intermedia, from an endemic area in Brazil. The adult insects were collected using CDC light traps, morphologically identified, carefully sterilized, dissected under a microscope and the females separated into groups according to their physiological condition: (i) absence of blood meal (unfed = UN); (ii) presence of blood meal (blood-fed = BF); and (iii) presence of developed ovaries (gravid = GR). Then, they were processed for metagenomics with Illumina Hiseq Sequencing in order to be sequence analyzed and to obtain the taxonomic profiles of the microbiota. Bacterial metagenomic analysis revealed differences in microbiota composition based upon the distinct physiological stages of the adult insect. Sequence identification revealed two phyla (Proteobacteria and Actinobacteria), 11 families and 15 genera; 87 % of the bacteria were Gram-negative, while only one family and two genera were identified as Gram-positive. The genera Ochrobactrum, Bradyrhizobium and Pseudomonas were found across all of the groups. The metagenomic analysis revealed that the microbiota of the Lu. intermedia female sand flies are distinct under specific physiological conditions and consist of 15 bacterial genera. The Ochrobactrum, Bradyrhizobium and Pseudomonas were the common genera. Our results detailing

  18. Intermedia transfer factors for fifteen toxic pollutants released to air basins in California

    Energy Technology Data Exchange (ETDEWEB)

    McKone, T.E.; Daniels, J.I. [Lawrence Livermore National Lab., CA (United States); Chiao, F.F.; Hsieh, D.P.H. [Univ. of California, Davis, CA (United States)

    1993-10-01

    This report provides a summary definition of the intermedia-transfer factors (ITFs). Methods are discussed for estimating these parameters in the absence of measured values, and the estimation errors inherent in these estimation methods are considered. A detailed summary is provided of measured and estimated ITF values for fifteen air contaminants. They include: 1,3 butadiene; cadmium; cellosolve; cellosolve acetate; chloroform; di-2-ethylhexylphthalate; 1,4-dioxame; hexachlorobenzene; inorganic arsenic; inorganic lead; nickel; tetrachloroethylene; toluene; toluene-2,4-diisocyanate; and 1,3-xylene. Recommendations are made regarding the expected value and variance in these values for use in exposure models.

  19. Production and characterization of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of Japanese bunching onion (A. fistulosum L.)

    OpenAIRE

    Hang, Tran Thi Minh; Shigyo, Masayoshi; Yamauchi, Naoki; Tashiro, Yosuke

    2004-01-01

    First and second backcrosses of amphidiploid hybrids (2n = 4x = 32, genomes AAFF) between shallot (Allium cepa Aggregatum group) and A. fistulosum were conducted to produce A. cepa - A. fistulosum alien addition lines. When shallot (A. cepa Aggregatum group) was used as a pollinator, the amphidiploids and allotriploids set germinable BC1 and BC2 seeds, respectively. The 237 BC1 plants mainly consisted of 170 allotriploids (2n = 3x = 24, AAF) and 42 hypo-allotriploids possessing 23 chromosomes...

  20. Antioxidant and schistosomicidal effect of Allium sativum and Allium cepa against Schistosoma mansoni different stages.

    Science.gov (United States)

    Mantawy, M M; Aly, H F; Zayed, N; Fahmy, Z H

    2012-07-01

    The schistosomicidal properties of garlic (Allium sativum) and onion (Allium cepa) powder were tested in vitro against Schistosoma mansoni miracidia, schistosomula, cercaria and adult worms. Results indicate their strong biocidal effects against all stages of the parasite and also show scavenging inhibitory effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO). In the present work, the in vivo effects of A. sativum and A. cepa on lipid peroxide and some antioxidant enzymes; thioredoxin reductase (TrxR), sorbitol dehydrogenase (SDH), superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) (as they have a crucial role in host protection against invading parasite) were also studied. The data demonstrate that, there was a significant inhibition in SOD, CAT, GR, TrxR and SDH in infected liver while, significant elevation was detected in lipid peroxide as compared to the normal control. The current resultS clearly revealed that, the used both edible plants enhance the host antioxidant system indicated by lowering in lipid peroxide and stimulation of SOD, CAT, GR, TrxR and SDH enzyme levels. Enhancement of such enzymes using A. sativum and A. cepa could in turn render the parasite vulnerable to damage by the host and may play a role in the antischistosomal potency of the used food ingredients.

  1. Combination of Cymbopogon citratus and Allium cepa essential oils increased antibacterial activity in leafy vegetables.

    Science.gov (United States)

    Ortega-Ramirez, Luis A; Silva-Espinoza, Brenda A; Vargas-Arispuro, Irasema; Gonzalez-Aguilar, Gustavo A; Cruz-Valenzuela, M Reynaldo; Nazzaro, Filomena; Ayala-Zavala, J Fernando

    2017-05-01

    Cymbopogon citratus and Allium cepa essential oils (EOs) are rich in terpenes and sulfur compounds respectively, both with antibacterial activity and different cell targets, supporting the idea that their combination can increase their efficacy. Major constituents of C. citratus were geranial and neral, while A. cepa presented dipropyl disulfide and dipropyl trisulfide. Cymbopogon citratus and A. cepa EOs inhibited the in vitro growth of Escherichia coli O157:H7 (minimal inhibitory concentrations of 2.21 and 5.13 g L -1 respectively), Salmonella Choleraesuis (3.04 and 1.28 g L -1 ), Listeria monocytogenes (1.33 and 2.56 g L -1 ) and Staphylococcus aureus (0.44 and 5.26 g L -1 ). Application of the EO combination to spinach caused a greater reduction in E. coli (2.34 log colony-forming units (CFU) g -1 ), S. Choleraesuis (2.94 log CFU g -1 ), L. monocytogenes (2.06 log CFU g -1 ) and S. aureus (1.37 log CFU g -1 ) compared with higher doses of individual EOs; a similar effect was observed for romaine lettuce. Individual and combined EOs caused a reduction in flavor acceptability level; however, no significant differences were found among odor acceptability of control vegetables and those treated with the EO combination and C. citratus EO. Leafy vegetables treated with the EO combination showed higher antibacterial protection and odor acceptability compared with individual EO treatments. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  2. TERAPIA FOTODINÂMICA COM AZUL DE METILENO SOBRE CEPA DE Staphylococcus aureus RESISTENTE À METICILINA

    Directory of Open Access Journals (Sweden)

    Mirian Alves Freitas

    2017-01-01

    Full Text Available S. aureus é o agente etiológico de maior importância associado às infecções adquiridas tanto em âmbito comunitário ou hospitalar, devido a sua capacidade de desenvolver resistência às terapias convencionais. A Terapia Fotodinâmica (TFD surge como alternativa a ser utilizada no controle de cepas resistentes aos antibioticos. O objetivo do presente estudo é avaliar os efeitos da TFD com Azul de Metileno (AM sobre cepas de S. aureus resistente à meticilina (MRSA. As amostras foram diluídas em PBS estéril de acordo com a escala 0,5 de MacFarland. Os grupos foram compostos com diferentes concentrações de AM, incubados no escuro durante 15 minutos e irradiados por LED Biopdi/Irrad-Led5 em 660nm com fluência de 10 J/cm². Posteriormente, foram semeadas e incubadas à 37ºC por 24 horas. A contagem de Unidades Formadoras de Colônias (UFCs demonstrou que a TFD foi eficaz na menor concentração testada de AM sobre cepas de MRSA.

  3. Selección de cepas de rizobios aisladas de ecosistemas ganaderos de Canadá, inoculadas en trigo (Triticum aestivum, L.. Selection of rhizobium strains isolated from livestock ecosystems of Canada, inoculated to wheat (Triticum aestivum L.

    Directory of Open Access Journals (Sweden)

    C.J Bécquer

    2007-06-01

    Full Text Available Se efectuó un experimento de campo con el objetivo de seleccionar cepas de rizobios inoculadas en trigo (Triticum aestivum, L., var. Cuba-204. Se evaluaron varios indicadoress agroproductivos de la planta, tales como: peso seco aéreo, longitud del tallo, rendimiento de granos, peso de 1000 granos y rendimiento de nitrógeno. Como criterio de selección de los mejores tratamientos se consideraron los valores estadísticamente superiores al control fertilizado. Se utilizaron cuatro cepas de referencia, pertenecientes a diferentes géneros y especies de rizobios, y 10 cepas nativas, pertenecientes al género Sinorhizobium, que fueron aisladas de raíces de leguminosas (Melilotus y Medicago, adaptadas a ecosistemas ganaderos de Alberta, Canadá. Una de las cepas fue aislada de leguminosas adaptadas a suelos contaminados con hidrocarburos de esa misma zona geográfica. Las cepas crecieron en LMA y resuspendidas en CLM hasta lograr una UFC de 10(6 _ 10(8 cél/mL. Se utilizaron métodos estándar para la inoculación de cereales. Se aplicó un diseño experimental de bloques completamente aleatorizado, con 16 tratamientos y cuatro réplicas. Uno de los tratamientos se fertilizó con 150 kg/ha (NH4NO3. Se utilizó análisis de varianza. Las diferencias entre medias fueron halladas por la prueba LSD de Fisher (pA field experiment was carried out in order to select rhizobial strains inoculated to wheat (Triticum aestivum, L., var. Cuba-204. Several parameters of agricultural significance were assessed, such as: aerial dry weight, length of stems, grain yield, weight of 1 000 grains and nitrogen yield. As selection criterion of the best treatments, the statistically higher values compared to the fertilized control were considered. Four reference strains, belonging to several rhizobial genus and species were used, as well as ten native strains, belonging to Sinorhizobium that were isolated from roots of legumes (Melilotus and Medicago, adapted to Canadian

  4. Determinación de las condiciones de crecimiento in vitro de una cepa probiótica (Lactobacillus delbruekii subsp. bulgaricus aislada del tracto intestinal de terneros (Bos taurus

    Directory of Open Access Journals (Sweden)

    José Ávila

    2010-07-01

    Full Text Available El objetivo de este estudio fue conocer el efecto de la temperatura, pH y la concentración de inóculo sobre el crecimiento in vitro de una cepa probiótica (Lactobacillus delbruekii subsp. bulgaricus aislada del intestino delgado de terneros. Para ello se utilizó en el primer caso, el método de superficie de respuesta para determinar las condiciones de pH y temperatura óptimas de la cepa, y en el segundo, un diseño experimental completamente aleatorizado considerando el factor concentración de inóculo inicial a seis niveles, donde el crecimiento (en medio De Man, Rogosa y Sharpe expresado en unidades de Absorbancia, constituyó la variable respuesta; presentándose una máxima respuesta (0,806 uA para 5 % de concentración inicial de inóculo. Los valores de pH y temperatura utilizados permitieron el establecimiento de una zona óptima de crecimiento in vitro en los siguientes intervalos, pH entre 5,1 y 5,6; y temperatura entre 38,0 y 41,5 °C, donde el máximo crecimiento se obtuvo a pH 5,31 y a 39,38 °C. Por otro lado se encontraron incrementos significativos del crecimiento in vitro (p < 0,05 a medida que se utiliza menor concentración de inóculo inicial. Con la información obtenida se espera incrementar los rendimientos de la cepa y así promover la producción de productos probióticos para el consumo animal en Venezuela.

  5. Determinación de las condiciones de crecimiento in vitro de una cepa probiótica (Lactobacillus delbruekii subsp. bulgaricus aislada del tracto intestinal de terneros (Bos taurus

    Directory of Open Access Journals (Sweden)

    José Ávila

    2010-06-01

    Full Text Available El objetivo de este estudio fue conocer el efecto de la temperatura, pH y la concentración de inóculo sobre el crecimiento in vitro de una cepa probiótica (Lactobacillus delbruekii subsp. bulgaricus aislada del intestino delgado de terneros. Para ello se utilizó en el primer caso, el método de superficie de respuesta para determinar las condiciones de pH y temperatura óptimas de la cepa, y en el segundo, un diseño experimental completamente aleatorizado considerando el factor concentración de inóculo inicial a seis niveles, donde el crecimiento (en medio De Man, Rogosa y Sharpe expresado en unidades de Absorbancia, constituyó la variable respuesta; presentándose una máxima respuesta (0,806 uA para 5 % de concentración inicial de inóculo. Los valores de pH y temperatura utilizados permitieron el establecimiento de una zona óptima de crecimiento in vitroen los siguientes intervalos, pH entre 5,1 y 5,6; y temperatura entre 38,0 y 41,5 °C, donde el máximo crecimiento se obtuvo a pH 5,31 y a 39,38 °C. Por otro lado se encontraron incrementos significativos del crecimiento in vitro (p < 0,05 a medida que se utiliza menor concentración de inóculo inicial. Con la información obtenida se espera incrementar los rendimientos de la cepa y así promover la producción de productos probióticos para el consumo animal en Venezuela.

  6. Secuenciación genética y mutaciones cromosómicas en cepas de Mycobacterium tuberculosis resistentes a rifampicina

    Directory of Open Access Journals (Sweden)

    Alexander Galindo

    2000-07-01

    Full Text Available Los individuos afectados con Mycobacterium tuberculosis son más de un billon y cerca de 3,5 millones enferman anualmente en el mundo. La Organizaci6n Mundial de la Salud (OMS considera que estarnos ante una emergencia global de tuberculosis que podría provocar 200 millones de casos nuevos y 70 millones de muertes antes del 2020. Se estima que en 1998 fueron reportados 8.8 millones de casos nuevos y que fallecieron por esta causa cerca de 3 millones de personas, 6% de la mortalidad global del año, La alta incidencia en la población, la convierte en la principal causa de muerte en adultos en edad productiva, es decir de 15 a 49 años. Se considera que 80% de la población está dentro del grupo de alto riesgo que puede padecer esta enfermedad y se asume que más de 50 millones de personas están infectadas con cepas resistentes (Guerrero,1998 y Rattan 1998.

  7. Leptospirosis en el Perú: I. Identificación de las cepas de leptospiras presentes en el perro y el gato e incidencia de la infección

    Directory of Open Access Journals (Sweden)

    Arístides Herrer

    1958-12-01

    Full Text Available Durante 1955 a 1957 se han realizado investigaciones acerca de la leptospirosis en el perro y el gato en la ciudad de Lima con la finalidad de determinar las cepas serológicas que infectan a dichos animales y la incidencia de la infección en relación con diversos factores. Todos los gatos y la mayoría de los perros estudiados procedían de la ciudad de Lima y sus inmediaciones; el resto de los perros, habían sido enviados a Lima procedentes de diversas localidades que se hallan a lo largo de la Costa. Las investigaciones efectuadas en dichos animales consisten en: a reacciones serológicas de aglutinación-lisis, en las que se empleó 11 cepas diferentes de leptospiras; b cultivos de la sangre y el riñón; y c observaciones al campo oscuro del triturado de riñón. Los principales resultados obtenidos son los siguientes: 1. De 444 perros en los que se realizaran las reacciones serológicas, en 206 (46.4% se ha verificado la presencia de anticuerpos a título que varió entre 1:100 a 1:30,000. Los anticuerpos verificados correspondieron casi en su totalidad a L. canícola Ruebush (84.5%, y L. icterohaemorrhagiae AB Winjberg (11.6%. 2. De los 206 perros con reacción serológica positiva, 66 (32% reaccionaron simultáneamente frente a dos cepas y 11 (5.3% frente a tres. En tales reacciones cruzadas la cepa L. ballum fue la que ofreció resultados positivos con mayor frecuencia (78.0% 3. En cuanto al título de los anticuerpos, en 154 casos (74.8% fue entre 1:100 y 1:300; en 49 (23.8%, entre 1:1,000 y 1:3,000; y en los tres restantes (1.4%, entre 1:10,000 y 1:30,000. 4. A semejanza de lo que sucede en otras partes, la incidencia de la leptospirosis canina es mayor durante los meses de temperatura más elevada (enero-marzo, del mismo modo, la incidencia es más alta en los animales machos (50% que en las hembras (35.9%. Ambos datos han sido obtenidos a base de los cultivos de riñón. 5. En 435 perros se hicieron cultivos de riñón y, en 113

  8. Producción de Biobutanol mediante fermentación ABE a partir de suero lácteo. (Efecto de suplementar el medio de fermentación con nutrientes esenciales)

    OpenAIRE

    Díez Antolínez, Rebeca

    2013-01-01

    Este trabajo tiene como principal objetivo mejorar el rendimiento y la productividad de la fermentación ABE en un proceso discontinuo, empleando suero bruto desproteinizado suplementado con nutrientes esenciales como sustrato y la cepa C. beijerinckii NCIMB 8052, como catalizador. Pruebas preliminares empleando suero bruto desproteinizado reportaron pequeñas concentraciones de solventes y bajas productividades y rendimientos. La suplementación del sustrato con concentraciones limitadas de nut...

  9. Cloning of a sesquiterpene synthase from Lavandula x intermedia glandular trichomes.

    Science.gov (United States)

    Sarker, Lukman S; Demissie, Zerihun A; Mahmoud, Soheil S

    2013-11-01

    The essential oil (EO) of Lavandula is dominated by monoterpenes, but can also contain small amounts of sesquiterpenes, depending on species and environmental conditions. For example, the sesquiterpene 9-epi-caryophyllene can make up to 8 % of the EO in a few species, including those commercially propagated for EO production. Here, we report the cloning and functional characterization of 9-epi-caryophyllene synthase (LiCPS) from the glandular trichomes of Lavandula x intermedia, cv. Grosso. The 1,617 bp open reading frame of LiCPS, which did not encode a transit peptide, was expressed in Escherichia coli and the recombinant protein purified by Ni-NTA agarose affinity chromatography. The ca. 60 kDa recombinant protein specifically converted farnesyl diphosphate to 9-epi-caryophyllene. LiCPS also produced a few monoterpenes when assayed with the monoterpene precursor geranyl diphosphate (GPP), but--unlike most monoterpene synthases--was not able to derive detectable amounts of any products from the cis isomer of GPP, neryl diphosphate. The LiCPS transcripts accumulated in developing L. x intermedia flowers and were highly enriched in glandular trichomes, but were not detected in leaves suggesting that the transcriptional expression of this gene is spatially and developmentally regulated.

  10. Molecular cloning and functional characterization of borneol dehydrogenase from the glandular trichomes of Lavandula x intermedia.

    Science.gov (United States)

    Sarker, Lukman S; Galata, Mariana; Demissie, Zerihun A; Mahmoud, Soheil S

    2012-12-15

    Several varieties of Lavandula x intermedia (lavandins) are cultivated for their essential oils (EOs) for use in cosmetic, hygiene and personal care products. These EOs are mainly constituted of monoterpenes including camphor, which contributes an off odor reducing the olfactory appeal of the oil. We have recently constructed a cDNA library from the glandular trichomes (the sites of EO synthesis) of L. x intermedia plants. Here, we describe the cloning of a borneol dehydrogenase cDNA (LiBDH) from this library. The 780 bp open reading frame of the cDNA encoded a 259 amino acid short chain alcohol dehydrogenase with a predicted molecular mass of ca. 27.5 kDa. The recombinant LiBDH was expressed in Escherichia coli, purified by Ni-NTA agarose affinity chromatography, and functionally characterized in vitro. The bacterially produced enzyme specifically converted borneol to camphor as the only product with K(m) and k(cat) values of 53 μM and 4.0 × 10(-4) s(-1), respectively. The LiBDH transcripts were specifically expressed in glandular trichomes of mature flowers indicating that like other Lavandula monoterpene synthases the expression of this gene is regulated in a tissue-specific manner. The cloning of LiBDH has far reaching implications in improving the quality of Lavandula EOs through metabolic engineering. Copyright © 2012 Elsevier Inc. All rights reserved.

  11. Morphology, ecology, and behaviour of Hylarana intermedia, a Western Ghats frog

    Directory of Open Access Journals (Sweden)

    Ambika Kamath

    2016-06-01

    Full Text Available Despite being common in the Western Ghats-Sri Lanka biodiversity hotspot, Golden-backed frogs (Hylarana, Ranidae remain poorly studied. In this paper, we present some preliminary data on the morphology, behaviour, and habitat use of Hylarana intermedia, a member of the Hylarana aurantiaca species group. We find evidence for female biased size dimorphism, as well as potential shape differences between the sexes in this species. Additionally, we investigate the relationships between traits that may contribute to male conspicuousness (call rates, dorsal coloration, and body size in two breeding habitats, a paddy field and a trench. Our results suggest both size-dependent and environment-dependent variation in call rate and colour in this species. Specifically, we find evidence against the adoption of sneaker mating strategies by small males in H. intermedia, and instead find variation both within and between populations in traits contributing to male conspicuousness. We conclude by proposing future directions for research on this common frog species.

  12. Spinal cord compression due to extramedullary hematopoiesis in beta-thalassemia intermedia

    International Nuclear Information System (INIS)

    Munn, Rita K.; Kramer, Carol A.; Arnold, Susanne M.

    1998-01-01

    Background: Extramedullary hematopoiesis (EMH) occurs in many disorders, including thalassemias and other hemoglobinopathies, and commonly presents in the spleen and liver. We present a case of spinal cord compression in a patient with beta-thalassemia intermedia, and review the literature and available treatment options. Patient and Methods: A 35-year-old black female with beta-thalassemia intermedia presented with a 3-week history of back pain and lower extremity weakness. Neurologic examination was consistent with spinal cord compression, and gadolinium enhanced magnetic resonance imaging (MRI) confirmed this diagnosis. She was given intravenous steroids and radiotherapy was begun in 200 cGy fractions to a total dose of 2000 cGy. Results: At the completion of radiotherapy the patient was ambulatory with mild residual weakness. MRI scans 16 months later showed smaller, but persistent masses, and she remains asymptomatic 5 years from her diagnosis. Conclusion: Recognition of spinal cord EMH requires prompt physical examination and MRI for accurate diagnosis. EMH can be managed with radiation, surgery, transfusions, or a combination of these therapies. Radiation in conservative doses of (750-3500 cGy) is non-invasive, avoids the surgical risks of potentially severe hemorrhage and incomplete resection, and has a high complete remission rate in the majority of patients. Relapse rates are moderate (37.5%), but retreatment provides excellent chance for second remission

  13. Noninvasive analysis of skin iron and zinc levels in beta-thalassemia major and intermedia

    International Nuclear Information System (INIS)

    Gorodetsky, R.; Goldfarb, A.; Dagan, I.; Rachmilewitz, E.A.

    1985-01-01

    Diagnostic x-ray spectrometry, a method based on x-ray fluorescence analysis, was used for noninvasive determination of iron and zinc in two distinct skin areas, representing predominantly dermal and epidermal tissues, in 56 patients with beta-thalassemia major and intermedia. The mean iron levels in the skin of patients with beta-thalassemia major and intermedia were elevated by greater than 200% and greater than 50%, respectively, compared with control values. The zinc levels of both skin areas examined were within the normal range. The data indicate that the rate and number of blood transfusions, which correlated well with serum ferritin levels (r . 0.8), are not the only factors that determine the amount of iron deposition in the skin (r less than 0.6). Other sources of iron intake contribute to the total iron load in the tissues, particularly in patients who are not given multiple transfusions. The noninvasive quantitation of skin levels may reflect the extent of iron deposition in major parenchymal organs. Repeated DXS examinations of the skin could monitor the clearance of iron from the tissues of patients with iron overload in the course of therapy with chelating agents

  14. Partial Splenectomy in the treatment of an adult with β thalassemia intermedia: A case report.

    Science.gov (United States)

    Correia, João Guardado; Moreira, Nídia; Costa Almeida, Carlos Eduardo; Reis, Luís Simões

    2017-01-01

    Thalassemia is a common disease which treatment is often based on splenectomy. The risks associated with total splenectomy stimulated partial splenectomy as a potentially alternative therapy. A 45 year-old female patient with long term follow-up for β thalassemia intermedia started to develop signs of hypersplenism and iron overload. A partial splenectomy was performed and was observed a marked hematologic improvement while preserving the desired splenic function. Partial splenectomy proved to provide a persistent decrease in hemolytic rate while preserving the integrity of splenic phagocytic function, presenting itself as an effective alternative to total splenectomy. After being subjected to partial splenectomy, our patient experienced a sustained control of hemolysis and showed no signs of hypersplenism or iron overload. No splenic regrowth or infectious complications were observed. The major drawbacks of partial splenectomy are the increased risk of intra- and postoperative bleeding, splenic remnant torsion and splenic regrowth. Partial splenectomy is an alternative to total splenectomy for the treatment of adult β Thalassemia intermedia patients avoiding the risks associated with total splenectomy.

  15. Detection of Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Prevotella nigrescens in chronic endodontic infection.

    Science.gov (United States)

    Tomazinho, Luiz Fernando; Avila-Campos, Mario J

    2007-02-01

    Black-pigmented anaerobic rods such as Prevotella spp. and Porphyromonas spp. are involved in the etiology and perpetuation of endodontic infections. The aim of this study was to evaluate the prevalence of these species in chronic endodontic infections by using culture and polymerase chain reaction (PCR) techniques. Samples of 100 patients with root canals displaying chronic endodontic infections were obtained by sterilized paper points. Bacterial identification was performed by using culture and PCR techniques. By culture, in 33% of the samples, P. intermedia-P. nigrescens (75.8%), P. gingivalis (27.3%), and P. endodontalis (9.1%) were identified, and by PCR 60% of the samples harbored P. nigrescens (43.3%), P. gingivalis (43.3%), P. intermedia (31.7%), and P. endodontalis (23.3%). The presence of these black-pigmented anaerobic rods alone or in association in chronic endodontic infections seems to be frequent. PCR is a very sensitive technique for detecting DNA from bacterial cells. Culturing is only able to reveal living bacteria and is less sensitive for the identification of low numbers of bacterial cells.

  16. Estudo de uma cepa humana de Schistosoma mansoni resistente a agentes esquistossomicidas

    Directory of Open Access Journals (Sweden)

    Naftale Katz

    1973-12-01

    Full Text Available Foi isolada uma cepa de Schistosoma mansoni proveniente de dois pacientes tratados com hycanthone, por duas vezes, na dose de 2,5mg/kg, i.m., em janeiro e em abril de 1970, e com niridazole (25mg/kg dia x 5 oral, em abril de 1971. O número de ovos por grama de fezes nestes pacientes antes do tratamento era de 2675 e 1025, respectivamente e, após o terceiro tratamento, em torno de 100 ovos/g. Miracídios obtidos das fezes destes pacientes, infectaram caramujos (Biomphalaria glabrata, que passaram a eliminar cercárias (cepa WW. Estas foram utilizadas para infecção experimental de camundongos albinos. Os animais infectados foram tratados com esquemas múltiplos de hycanthone, niridazole e oxamniquine. Estudos comparativos das cepas WW e LE (esta última mantida rotineiramente em nossos laboratórios mostraram diferenças acentuadas quando à sensibilidade aos esquistossomicidas usados. De fato, com hycanthone, na dose de 80mg/kg, i.m. houve 100% de alteração do oograma nos camundongos infectados com a cepa L.E. e de 0,0% nos infectados com a cepa WW. Com a oxamniquine e niridazole as diferenças foram menores, mas, ainda assim, suficientes para indicar maior resistência da cepa WW a estes esquistossomicidas. Esta é a primeira vez na literatura, que se demonstra resistência em cepas de S. mansoni provenientes de pacientes tratados.There has been isolated a Schistosoma mansoni strain from two patients submitted to two courses of treatment with hycanthone (2,5mg/kg, i.m., in January and April, 1970, and to one course with niridazole (25mg/kg/day x 5, per os, in April, 1971. Before treatment, the number of eggs in the faeces of those patients was, per gram, 2,675 and 1,025, respectively; after completion of treatment, such number had come down to around 100 eggs/gram. Miracidia hatched from the patients faeces could infect Biomphalaria glabrata snails, which elimmated cercariae (WW strain that were used for experimental infection of albino

  17. Estudo da competência vetorial de Lutzomyia intermedia (Lutz & Neiva, 1912 para Leishmania (Viannia braziliensis, Vianna, 1911 Study of the vectorial competence of Lutzomyia intermedia (Lutz & Neiva, 1912 to Leishmania (Viannia braziliensis, Vianna, 1911

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    Antonio Carlos da Silva

    2001-04-01

    Full Text Available Estudou-se a competência vetorial de Lutzomyia intermedia (Diptera: Psychodidae do Vale do Ribeira (SP para estirpes de Leishmania (Viannia braziliensis (Kinetoplastida: Trypanosomatidae, mediante pesquisa de infectividade natural; exposições de fêmeas silvestres e colonizadas (F1 às lesões de hamsters experimentalmente infectados e testes de transmissão via picada. A infectividade natural e os testes de transmissão revelaram-se negativos e, nas exposições, foram obtidas positividades de 74% (123+/166 dissecados e 70% (115+/164 dissecados para fêmeas silvestres e colonizadas respectivamente, e o desenvolvimento das formas evolutivas compatíveis com o modelo Peripilaria. A suscetibilidade às estirpes testadas associada aos indicadores epidemiológicos concorrem para a suspeita do papel vetorial de Lutzomyia intermedia na região estudada.This paper investigated the vectorial competence of Lutzomyia intermedia (Diptera: Psychodidae in Vale do Ribeira (SP to strains of Leishmania (Viannia braziliensis (Kinetoplastida: Trypanosomatidae, by means of a search for natural infection; exposure of wild and colonized females (F1 to the lesions of experimentally infected hamsters and transmission tests by bite. The natural infection and the transmission tests were negative. In the exposures of Lu. intermedia to infected lesions we found rates of 74% (123+/166 dissected and 70% (115+/164 dissected for the wild and colonized females respectively. The development of the parasites was compatible with the development model of Peripilaria. The susceptibility of the tested strains associated with the epidemiological indicators contribute to the vectorial role suspicion of Lutzomyia intermedia in the studied region.

  18. Complete genome sequence of Brachyspira intermedia reveals unique genomic features in Brachyspira species and phage-mediated horizontal gene transfer

    Science.gov (United States)

    2011-01-01

    Background Brachyspira spp. colonize the intestines of some mammalian and avian species and show different degrees of enteropathogenicity. Brachyspira intermedia can cause production losses in chickens and strain PWS/AT now becomes the fourth genome to be completed in the genus Brachyspira. Results 15 classes of unique and shared genes were analyzed in B. intermedia, B. murdochii, B. hyodysenteriae and B. pilosicoli. The largest number of unique genes was found in B. intermedia and B. murdochii. This indicates the presence of larger pan-genomes. In general, hypothetical protein annotations are overrepresented among the unique genes. A 3.2 kb plasmid was found in B. intermedia strain PWS/AT. The plasmid was also present in the B. murdochii strain but not in nine other Brachyspira isolates. Within the Brachyspira genomes, genes had been translocated and also frequently switched between leading and lagging strands, a process that can be followed by different AT-skews in the third positions of synonymous codons. We also found evidence that bacteriophages were being remodeled and genes incorporated into them. Conclusions The accessory gene pool shapes species-specific traits. It is also influenced by reductive genome evolution and horizontal gene transfer. Gene-transfer events can cross both species and genus boundaries and bacteriophages appear to play an important role in this process. A mechanism for horizontal gene transfer appears to be gene translocations leading to remodeling of bacteriophages in combination with broad tropism. PMID:21816042

  19. Characterization of pars intermedia connections in amphibians by biocytin tract tracing and immunofluorescence aided by confocal microscopy

    NARCIS (Netherlands)

    Jansen, K; Fabro, C; Artero, C; Feuilloley, M; Vaudry, H; Fasolo, A; Franzoni, MF

    Biocytin, recently introduced in neuroanatomical studies, was used as a retrograde tract tracer in combination with immunofluorescence in order to analyse the neurochemical characters of some central neuronal projections to the pars intermedia in two amphibian species, the anuran Rana esculenta and

  20. In vitro effects of N-acetyl cysteine alone and in combination with antibiotics on Prevotella intermedia.

    Science.gov (United States)

    Moon, Ji-Hoi; Jang, Eun-Young; Shim, Kyu Sang; Lee, Jin-Yong

    2015-05-01

    N-acetyl cysteine (NAC) is an antioxidant that possesses anti-inflammatory activities in tissues. In the field of dentistry, NAC was demonstrated to prevent the expression of LPS-induced inflammatory mediators in phagocytic cells and gingival fibroblasts during the inflammatory process, but the effect of NAC on oral pathogens has been rarely studied. Here, we examined the effect of NAC against planktonic and biofilm cells of Prevotella intermedia, a major oral pathogen. NAC showed antibacterial activity against the planktonic P. intermedia with MIC value of 3 mg/ml and significantly decreased biofilm formation by the bacterium even at sub MIC. NAC did not affect the antibiotic susceptibility of planktonic P. intermedia, showing indifference (fractional inhibitory concentration index of 0.5-4) results against the bacterium in combination with ampicillin, ciprofloxacin, tetracycline or metronidazole. On the other hand, viability of the pre-established bacterial biofilm exposed to the antibiotics except metronidazole was increased in the presence of NAC. Collectively, NAC may be used for prevention of the biofilm formation by P. intermedia rather than eradication of the pre-established bacterial biofilm. Further studies are required to explore antibacterial and anti-biofilm activity of NAC against mixed population of oral bacteria and its modulatory effect on antibiotics used for oral infectious diseases.

  1. Does estradiol have an impact on the dipeptidyl peptidase IV enzyme activity of the Prevotella intermedia group bacteria?

    Science.gov (United States)

    Fteita, Dareen; Könönen, Eija; Gürsoy, Mervi; Söderling, Eva; Gürsoy, Ulvi Kahraman

    2015-12-01

    Initiation and development of pregnancy-associated gingivitis is seemingly related to the microbial shift towards specific gram-negative anaerobes in subgingival biofilms. It is known that Prevotella intermedia sensu lato is able to use estradiol as an alternative source of growth instead of vitamin K. The aim of the present study was to investigate the impact of estradiol on the bacterial dipeptidyl peptidase IV (DPPIV) enzyme activity in vitro as a virulent factor of the Prevotella intermedia group bacteria, namely P. intermedia, Prevotella nigrescens, Prevotella pallens, and Prevotella aurantiaca. In all experiments, 2 strains of each Prevotella species were used. Bacteria were incubated with the concentrations of 0, 30, 90, and 120 nmol/L of estradiol and were allowed to build biofilms at an air-solid interface. DPPIV activities of biofilms were measured kinetically during 20 min using a fluorometric assay. The enzyme activity was later related to the amount of protein produced by the same biofilm, reflecting the biofilm mass. Estradiol significantly increased DPPIV activities of the 8 Prevotella strains in a strain- and dose-dependent manner. In conclusion, our in vitro experiments indicate that estradiol regulates the DPPIV enzyme activity of P. intermedia, P. nigrescens, P. pallens, and P. aurantiaca strains differently. Our results may, at least partly, explain the role of estradiol to elicit a virulent state which contributes to the pathogenesis of pregnancy-related gingivitis. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan.

    Science.gov (United States)

    Gul, Rahman; Jan, Syed Umer; Faridullah, Syed; Sherani, Samiullah; Jahan, Nusrat

    2017-01-01

    The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the E. intermedia to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in E. intermedia . The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the E. intermedia plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in E. intermedia was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).

  3. Equine pituitary pars intermedia dysfunction: current understanding and recommendations from the Australian and New Zealand Equine Endocrine Group.

    Science.gov (United States)

    Secombe, C J; Bailey, S R; de Laat, M A; Hughes, K J; Stewart, A S; Sonis, J M; Tan, Rhh

    2018-06-03

    The purpose of this article is to provide a review of the current knowledge and opinions about the epidemiology, clinical findings (including sequelae), diagnosis, treatment and monitoring of equine pituitary pars intermedia dysfunction, particularly in the Australian context. This information and the recommendations provided will assist practitioners in making informed decisions regarding the diagnosis and management of this disorder. © 2018 Australian Veterinary Association.

  4. Genetic and Pathogenic Variability of Fusarium oxysporum f. sp. cepae Isolated from Onion and Welsh Onion in Japan.

    Science.gov (United States)

    Sasaki, Kazunori; Nakahara, Katsuya; Tanaka, Shuhei; Shigyo, Masayoshi; Ito, Shin-ichi

    2015-04-01

    Fusarium oxysporum f. sp. cepae causes Fusarium basal rot in onion (common onion) and Fusarium wilt in Welsh onion. Although these diseases have been detected in various areas in Japan, knowledge about the genetic and pathogenic variability of F. oxysporum f. sp. cepae is very limited. In this study, F. oxysporum f. sp. cepae was isolated from onion and Welsh onion grown in 12 locations in Japan, and a total of 55 F. oxysporum f. sp. cepae isolates (27 from onion and 28 from Welsh onion) were characterized based on their rDNA intergenic spacer (IGS) and translation elongation factor-1α (EF-1α) nucleotide sequences, vegetative compatibility groups (VCGs), and the presence of the SIX (secreted in xylem) homologs. Phylogenetic analysis of IGS sequences showed that these isolates were grouped into eight clades (A to H), and 20 onion isolates belonging to clade H were monophyletic and assigned to the same VCG. All the IGS-clade H isolates possessed homologs of SIX3, SIX5, and SIX7. The SIX3 homolog was located on a 4 Mb-sized chromosome in the IGS-clade H isolates. Pathogenicity tests using onion seedlings showed that all the isolates with high virulence were in the IGS-clade H. These results suggest that F. oxysporum f. sp. cepae isolates belonging to the IGS-clade H are genetically and pathogenically different from those belonging to the other IGS clades.

  5. Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors.

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    Graziela Murta Barbosa

    Full Text Available It is well known that strain and virulence diversity exist within the population structure of Porphyromonas gingivalis. In the present study we investigate intra- and inter-species variability in biofilm formation of Porphyromonas gingivalis and partners Prevotella intermedia and Prevotella nigrescens. All strains tested showed similar hydrophobicity, except for P. gingivalis W83 which has roughly half of the hydrophobicity of P. gingivalis ATCC33277. An intraspecies variability in coaggregation of P. gingivalis with P. intermedia was also found. The association P. gingivalis W83/P. intermedia 17 produced the thickest biofilm and strain 17 was prevalent. In a two-compartment system P. gingivalis W83 stimulates an increase in biomass of strain 17 and the latter did not stimulate the growth of P. gingivalis W83. In addition, P. gingivalis W83 also stimulates the growth of P. intermedia ATCC25611 although strain W83 was prevalent in the association with P. intermedia ATCC25611. P. gingivalis ATCC33277 was prevalent in both associations with P. intermedia and both strains of P. intermedia stimulate the growth of P. gingivalis ATCC33277. FISH images also showed variability in biofilm structure. Thus, the outcome of the association P. gingivalis/P. intermedia seems to be strain-dependent, and both soluble factors and physical contact are relevant. The association P. gingivalis-P. nigrescens ATCC33563 produced larger biomass than each monotypic biofilm, and P. gingivalis was favored in consortia, while no differences were found in the two-compartment system. Therefore, in consortia P. gingivalis-P. nigrescens physical contact seems to favor P. gingivalis growth. The intraspecies variability found in our study suggests strain-dependence in ability of microorganisms to recognize molecules in other bacteria which may further elucidate the dysbiosis event during periodontitis development giving additional explanation for periodontal bacteria, such as P

  6. Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors.

    Science.gov (United States)

    Barbosa, Graziela Murta; Colombo, Andrea Vieira; Rodrigues, Paulo Henrique; Simionato, Maria Regina Lorenzetti

    2015-01-01

    It is well known that strain and virulence diversity exist within the population structure of Porphyromonas gingivalis. In the present study we investigate intra- and inter-species variability in biofilm formation of Porphyromonas gingivalis and partners Prevotella intermedia and Prevotella nigrescens. All strains tested showed similar hydrophobicity, except for P. gingivalis W83 which has roughly half of the hydrophobicity of P. gingivalis ATCC33277. An intraspecies variability in coaggregation of P. gingivalis with P. intermedia was also found. The association P. gingivalis W83/P. intermedia 17 produced the thickest biofilm and strain 17 was prevalent. In a two-compartment system P. gingivalis W83 stimulates an increase in biomass of strain 17 and the latter did not stimulate the growth of P. gingivalis W83. In addition, P. gingivalis W83 also stimulates the growth of P. intermedia ATCC25611 although strain W83 was prevalent in the association with P. intermedia ATCC25611. P. gingivalis ATCC33277 was prevalent in both associations with P. intermedia and both strains of P. intermedia stimulate the growth of P. gingivalis ATCC33277. FISH images also showed variability in biofilm structure. Thus, the outcome of the association P. gingivalis/P. intermedia seems to be strain-dependent, and both soluble factors and physical contact are relevant. The association P. gingivalis-P. nigrescens ATCC33563 produced larger biomass than each monotypic biofilm, and P. gingivalis was favored in consortia, while no differences were found in the two-compartment system. Therefore, in consortia P. gingivalis-P. nigrescens physical contact seems to favor P. gingivalis growth. The intraspecies variability found in our study suggests strain-dependence in ability of microorganisms to recognize molecules in other bacteria which may further elucidate the dysbiosis event during periodontitis development giving additional explanation for periodontal bacteria, such as P. gingivalis and P

  7. Anuncios y estrategias de campaña en televisión: un balance de la elección intermedia de 2015 en México

    Directory of Open Access Journals (Sweden)

    Oniel Francisco Díaz Jiménez

    2016-10-01

    Full Text Available En las campañas electorales profesionalizadas, los partidos políticos y los candidatos presentan sus propuestas ante el electorado y buscan maximizar votos, principalmente, a través de los medios de comunicación. En elecciones presidenciales, las estrategias y las tácticas de campaña se enfocan en las imágenes y las propuestas políticas de los candidatos que compiten por la Presidencia. Sin embargo, ¿qué ocurre en elecciones congresionales intermedias, donde los actores principales de la campaña son los partidos políticos y sus candidatos que compiten por cargos legislativos? Este trabajo realiza un análisis de contenido de los anuncios televisivos (spots de 30 segundos transmitidos por los partidos mexicanos durante las elecciones congresionales intermedias de 2015, con el fin de identificar sus estrategias de campaña. Los resultados muestran diferencias relevantes entre las campañas realizadas por las organizaciones nacionales de los partidos y las campañas de los candidatos individuales a nivel distrital. Sin embargo, también ponen a la vista que los anuncios de ataque fueron un componente central en las estrategias mediáticas de los partidos y los candidatos durante la campaña. / In professionalized election campaigns, political parties and candidates present their policy proposals to the electorate and seek to maximize votes, primarily through media appeals. In presidential elections, campaign tactics and strategies focus on the images and policy proposals of presidential candidates. However, what happens in congressional midterm elections, where the main campaigners are the political parties and their congressional candidates? This article analyzes the content of all television announcements (30-second spots broadcast by Mexican political parties during the 2015 mid-term congressional elections, in order to identify their campaign strategies. The results show relevant differences between the campaigns run by national

  8. NUTRICIÓN MINERAL CON N, P Y K EN LA SIMBIOSIS HONGOS MICORRIZÓGENOS-TOMATE (Lycopersicon esculentum Mill.) EN FERRALSOLS

    OpenAIRE

    Desirée Llonín; N. Medina

    2002-01-01

    Se evaluó el efecto de diferentes relaciones de nutrientes con N, P y K y la inoculación con dos cepas de hongos micorrízicos arbusculares-HMA- (G. clarum y G. fasciculatum), como fuentes de suministro de nutrientes aplicadas solas o en combinación, en fase de campo, sobre los incrementos de los rendimientos y la absorción de nutrientes. Para ello se determinaron el rendimiento del cultivo, la absorción de nutrientes y la colonización por HMA. Los resultados indican que la inoculación con hon...

  9. Medio de cultivo utilizando residuos-sólidos para el crecimiento de una bacteria nativa con potencial biofertilizante

    OpenAIRE

    Lara Mantilla, Cecilia; García Támara, Liliana Pahola; Oviedo Zumaqué, Luis E

    2010-01-01

    En el presente trabajo se muestran los resultados obtenidos de la evaluación del crecimiento, desarrollo y viabilidad de una cepa bacteriana nativa Azotobacter A15M2G con potencial biofertilizante, sobre un medio de cultivo preparado con residuos sólidos vegetales procedentes del mercado: Brassica Oleracea (repollo), Lactusa sativa (lechuga) y Allium fistulosum (cebollín). El crecimiento de la bacteria en el medio de residuo vegetal a diferentes concentraciones: 25, 50 y 75% p/v fue evaluado,...

  10. Inhibition of the growth of Ascosphaera apis by Bacillus and Paenibacillus strains isolated from honey Inhibición del crecimiento de Ascosphaera apis mediante cepas de Bacillus y Paenibacillus aisladas de miel

    Directory of Open Access Journals (Sweden)

    F. J. Reynaldi

    2004-03-01

    seleccionadas, se empleó la técnica de cultivo dual con 5 repeticiones de cada combinación antagonista bacteriano / cepa de A. apis, 5 para cada control y 4 medios de cultivo diferentes empleando 10 cepas del hongo de distintos orígenes geográficos. El análisis de la variancia y posterior comparación de medias LSD (Least Square Dfferences mostró que los mejores antagonistas fueron B. subtilis (m329 y B. megaterium (m435 para el caso del medio YGPSA, mientras que para MYPGP las más eficientes fueron las cepas de B. circulans Fr 231 y m448b.

  11. Different water use strategies of juvenile and adult Caragana intermedia plantations in the Gonghe Basin, Tibet Plateau.

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    Zhiqing Jia

    Full Text Available In a semi-arid ecosystem, water is one of the most important factors that affect vegetation dynamics, such as shrub plantation. A water use strategy, including the main water source that a plant species utilizes and water use efficiency (WUE, plays an important role in plant survival and growth. The water use strategy of a shrub is one of the key factors in the evaluation of stability and sustainability of a plantation.Caragana intermedia is a dominant shrub of sand-binding plantations on sand dunes in the Gonghe Basin in northeastern Tibet Plateau. Understanding the water use strategy of a shrub plantation can be used to evaluate its sustainability and long-term stability. We hypothesized that C. intermedia uses mainly deep soil water and its WUE increases with plantation age. Stable isotopes of hydrogen and oxygen were used to determine the main water source and leaf carbon isotope discrimination was used to estimate long-term WUE. The root system was investigated to determine the depth of the main distribution. The results showed that a 5-year-old C. intermedia plantation used soil water mainly at a depth of 0-30 cm, which was coincident with the distribution of its fine roots. However, 9- or 25-year-old C. intermedia plantations used mainly 0-50 cm soil depth water and the fine root system was distributed primarily at soil depths of 0-50 cm and 0-60 cm, respectively. These sources of soil water are recharged directly by rainfall. Moreover, the long-term WUE of adult plantations was greater than that of juvenile plantations.The C. intermedia plantation can change its water use strategy over time as an adaptation to a semi-arid environment, including increasing the depth of soil water used for root growth, and increasing long-term WUE.

  12. Different Water Use Strategies of Juvenile and Adult Caragana intermedia Plantations in the Gonghe Basin, Tibet Plateau

    Science.gov (United States)

    Jia, Zhiqing; Zhu, Yajuan; Liu, Liying

    2012-01-01

    Background In a semi-arid ecosystem, water is one of the most important factors that affect vegetation dynamics, such as shrub plantation. A water use strategy, including the main water source that a plant species utilizes and water use efficiency (WUE), plays an important role in plant survival and growth. The water use strategy of a shrub is one of the key factors in the evaluation of stability and sustainability of a plantation. Methodology/Principal Findings Caragana intermedia is a dominant shrub of sand-binding plantations on sand dunes in the Gonghe Basin in northeastern Tibet Plateau. Understanding the water use strategy of a shrub plantation can be used to evaluate its sustainability and long-term stability. We hypothesized that C. intermedia uses mainly deep soil water and its WUE increases with plantation age. Stable isotopes of hydrogen and oxygen were used to determine the main water source and leaf carbon isotope discrimination was used to estimate long-term WUE. The root system was investigated to determine the depth of the main distribution. The results showed that a 5-year-old C. intermedia plantation used soil water mainly at a depth of 0–30 cm, which was coincident with the distribution of its fine roots. However, 9- or 25-year-old C. intermedia plantations used mainly 0–50 cm soil depth water and the fine root system was distributed primarily at soil depths of 0–50 cm and 0–60 cm, respectively. These sources of soil water are recharged directly by rainfall. Moreover, the long-term WUE of adult plantations was greater than that of juvenile plantations. Conclusions The C. intermedia plantation can change its water use strategy over time as an adaptation to a semi-arid environment, including increasing the depth of soil water used for root growth, and increasing long-term WUE. PMID:23029303

  13. Diploid hybrid origin of Ostryopsis intermedia (Betulaceae) in the Qinghai-Tibet Plateau triggered by Quaternary climate change.

    Science.gov (United States)

    Liu, Bingbing; Abbott, Richard J; Lu, Zhiqiang; Tian, Bin; Liu, Jianquan

    2014-06-01

    Despite the well-known effects that Quaternary climate oscillations had on shaping intraspecific diversity, their role in driving homoploid hybrid speciation is less clear. Here, we examine their importance in the putative homoploid hybrid origin and evolution of Ostryopsis intermedia, a diploid species occurring in the Qinghai-Tibet Plateau (QTP), a biodiversity hotspot. We investigated interspecific relationships between this species and its only other congeners, O. davidiana and O. nobilis, based on four sets of nuclear and chloroplast population genetic data and tested alternative speciation hypotheses. All nuclear data distinguished the three species clearly and supported a close relationship between O. intermedia and the disjunctly distributed O. davidiana. Chloroplast DNA sequence variation identified two tentative lineages, which distinguished O. intermedia from O. davidiana; however, both were present in O. nobilis. Admixture analyses of genetic polymorphisms at 20 SSR loci and sequence variation at 11 nuclear loci and approximate Bayesian computation (ABC) tests supported the hypothesis that O. intermedia originated by homoploid hybrid speciation from O. davidiana and O. nobilis. We further estimated that O. davidiana and O. nobilis diverged 6-11 Ma, while O. intermedia originated 0.5-1.2 Ma when O. davidiana is believed to have migrated southward, contacted and hybridized with O. nobilis possibly during the largest Quaternary glaciation that occurred in this region. Our findings highlight the importance of Quaternary climate change in the QTP in causing hybrid speciation in this important biodiversity hotspot. © 2014 John Wiley & Sons Ltd.

  14. Aislamiento y caracterización de cepas de rizobios aisladas de diferentes leguminosas en la región de Cascajal, Villa Clara Isolation and characterization of rhizobia strains isolated from different legumes in the Cascajal region, Villa Clara

    Directory of Open Access Journals (Sweden)

    Pérez Guianeya

    2008-06-01

    Full Text Available Se aislaron 19 cepas de rizobio a partir de los nódulos de las leguminosas Canavalia ensiformis, Stylosanthes guianensis, Centrosema molle, Pueraria phaseoloides y Macroptilium atropurpureum, con el objetivo de obtener inoculantes efectivos para dichos cultivos en suelos ácidos. Para la caracterización de los aislados bacterianos se estudiaron sus características micromorfológicas, tintoriales y culturales, así como algunas respuestas fisiológico-bioquímicas, tales como la excreción de ácido o base al medio de cultivo y el ensayo de la cetolactasa. Se hizo un ensayo de nodulación in vitro con cada una de las leguminosas para determinar la efectividad de los aislados. De acuerdo con las características culturales, la tasa de crecimiento en medio LMA y la producción de ácido o base, los aislados obtenidos pudieran pertenecer a los géneros Bradyrhizobium y Rhizobium y/o Sinorhizobium. La prueba de la cetolactasa permitió conocer que ninguno de los aislados en estudio pertenece al género Agrobacterium. El ensayo con plantas inoculadas mostró que todas las cepas aisladas fueron efectivas en la nodulación, ya que nodularon en las leguminosas probadas. Se concluye que de las cepas aisladas 11 pudieran pertenecer al género Bradyrhizobium, mientras que las ocho restantes comparten características similares con los géneros Rhizobium y/o Sinorhizobium; todos los aislados fueron efectivos en la nodulación y pueden considerarse cepas promisorias para la obtención de inoculantes para las leguminosas forrajeras en estudio.Nineteen rhizobium strains were isolated from the nodules of the legumes Canavalia ensiformis, Stylosanthes guianensis, Centrosema molle, Pueraria phaseoloides and Macroptilium atropurpureum, with the objective of obtaining effective inoculants for such crops in acid soils. For the characterization of the bacterial isolates, their micromorphological, staining and cultural characteristics were studied, as well as some

  15. Screening and incorporation of rust resistance from Allium cepa into bunching onion (Allium fistulosum) via alien chromosome addition.

    Science.gov (United States)

    Wako, Tadayuki; Yamashita, Ken-ichiro; Tsukazaki, Hikaru; Ohara, Takayoshi; Kojima, Akio; Yaguchi, Shigenori; Shimazaki, Satoshi; Midorikawa, Naoko; Sakai, Takako; Yamauchi, Naoki; Shigyo, Masayoshi

    2015-04-01

    Bunching onion (Allium fistulosum L.; 2n = 16), bulb onion (Allium cepa L. Common onion group), and shallot (Allium cepa L. Aggregatum group) cultivars were inoculated with rust fungus, Puccinia allii, isolated from bunching onion. Bulb onions and shallots are highly resistant to rust, suggesting they would serve as useful resources for breeding rust resistant bunching onions. To identify the A. cepa chromosome(s) related to rust resistance, a complete set of eight A. fistulosum - shallot monosomic alien addition lines (MAALs) were inoculated with P. allii. At the seedling stage, FF+1A showed a high level of resistance in controlled-environment experiments, suggesting that the genes related to rust resistance could be located on shallot chromosome 1A. While MAAL, multi-chromosome addition line, and hypoallotriploid adult plants did not exhibit strong resistance to rust. In contrast to the high resistance of shallot, the addition line FF+1A+5A showed reproducibly high levels of rust resistance.

  16. Report on the CEPA activities [Consorcio Educativo para la Proteccion Ambiental/Educational Consortium for Environmental Preservation] [Final report of activities from 1998 to 2002

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, Miriam

    2003-02-01

    This report compiles the instances of scientific, educational, and institutional cooperation on environmental issues and other activities in which CEPA was engaged during the past five years, and includes several annual reports and meeting summaries. CEPA is a collaborative international consortium that brings together higher education institutions with governmental agencies, research laboratories, and private sector entities. CEPA's mission is to strengthen the technical, professional, and educational environmental infrastructure in the United States and Latin America. The CEPA program includes curriculum development, student exchange, faculty development, and creation of educational materials, joint research, and other cooperative activities. CEPA's goals are accomplished by actively working with Hispanic-serving institutions of higher education in the United States, in collaboration with institutions of higher education in Latin America and other Consortium members to deliver competitive environmental programs.

  17. Posibles efectos en la salud asociados con la metalurgia precolombina.

    Directory of Open Access Journals (Sweden)

    Alvaro Javier Idrovo

    2005-09-01

    Full Text Available En el Viejo Mundo algunos investigadores piensan que los efectos adversos en la salud relacionados con la exposición a arsénico influyeron para que se cambiara de usar aleaciones de cobre con arsénico a otras menos tóxicas. En este artículo se evalúa esta hipótesis para las tres grandes tradiciones metalúrgicas precolombinas: Andes centrales, área intermedia y occidente mexicano. Los artefactos metálicos revelan que las concentraciones de arsénico en los Andes centrales fueron similares a las del Viejo Mundo (0,5% a 1%, en el área intermedia los valores eran muy inferiores, mientras en el occidente mexicano fueron muy superiores (7% a 25%. En los Andes centrales se observó inicialmente el uso de bronce arsenical, pero rápidamente se conocieron las aleaciones de cobre-estaño; estas últimas fueron cada vez más preferidas y difundidas por todo el imperio inca. Las evidencias, osteológicas y en objetos artísticos, de amputaciones de los pies entre individuos moches de los Andes centrales apoyan la idea de la presencia de la ?enfermedad del pie negro? entre las poblaciones precolombinas. En conclusión, es posible que los efectos nocivos del arsénico se hayan observado en el Nuevo Mundo, y favorecido el cambio hacia aleaciones menos tóxicas. Se requieren nuevos estudios específicos para verificar esta hipótesis.

  18. Intermediação no futebol profissional : regime e âmbitos contratuais

    OpenAIRE

    Marques, Fernanda Duarte de Barros, 1973-

    2018-01-01

    Dissertação de mestrado em Direito, Universidade Lusíada de Lisboa, 2017 Exame público realizado em 23 de Janeiro de 2018 A presente dissertação de mestrado tem por objecto nuclear a análise do regime jurídico da actividade do empresário de futebol e do respectivo contrato que este firma com os jogadores de futebol ou com os clubes/sociedades desportivas vocacionado para a sua intermediação na celebração de contratos desportivos. A versátil, elástica e complexa actividade do empresário ...

  19. [Phylogenetic diversity of microorganisms associated with the deep-water sponge Baikalospongia intermedia].

    Science.gov (United States)

    Kalyzhnaya, O V; Itskovich, V B

    2014-07-01

    The diversity of bacteria associated with deep-water sponge Baikalospongia intermedia was evaluated by sequence analysis of 16S rRNA genes from two sponge samples collected in Lake Baikal from depths of 550 and 1204 m. A total of 64 operational taxonomic units, belonging to nine bacterial phyla, Proteobacteria (classes Alphaproteobacteria,. Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria), Actinobacteria, Planctomycetes, Cloroflexi, Verrucomicrobia, Acidobacteria, Chlorobi, and Nitrospirae, including candidate phylum WS5, were identified. Phylogenetic analysis showed that the examined communities contained phylotypes exhibiting homology to uncultured bacteria from different lake ecosystems, freshwater sediments, soil and geological formations. Moreover, a number of phylotypes were relative to psychrophilic, methane-oxidizing, sulfate-reducing bacteria, and to microorganisms resistant to the influence of heavy metals. It seems likely that the unusual habitation conditions of deep-water sponges contribute to the taxonomic diversity of associated bacteria and have an influence on the presence of functionally important microorganisms in bacterial communities.

  20. In Vitro Antibacterial and Antifungal Activity of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’

    Directory of Open Access Journals (Sweden)

    Sanda Vladimir-Knezevic

    2011-05-01

    Full Text Available This study aimed to evaluate the in vitro antibacterial and antifungal activities of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’, an indigenous Croatian cultivar of lavandin. For that purpose the activity of ethanolic extracts of flowers, inflorescence stalks and leaves against thirty one strains of bacteria, yeasts, dermatophytes and moulds were studied using both the agar well diffusion and broth dilution assays. Among the investigated extracts found to be effective against a broad spectrum of microorganisms, the flower extract was considered to be the most potent one. Linalool and rosmarinic acid, as the most abundant constituents found, are very likely major contributors to the observed antimicrobial effects. The results suggest that flowers of lavandin ‘Budrovka’ could serve as a rich source of natural terpene and polyphenol antimicrobial agents.

  1. Networked Intermedia Agenda Setting: The Geography of a Hyperlinked Scandinavian News Ecology

    DEFF Research Database (Denmark)

    Sjøvaag, Helle; Stavelin, Eirik; Karlsson, Michael

    How does agenda setting work within the hyperlinked Scandinavian news ecology? This paper investigates intermedia agenda setting within and between the local, regional, national and supra-national levels in Sweden, Denmark and Norway; analyses the center/periphery dimensions of hyperlink connecti......, social geography and hyperlinked news agendas in Scandinavia, adding to the research on the political implications of the Internet on national public spheres....... March 2016, amounting to approximately 2 million hyperlinks, each geotagged with publication origin. The visualisation of the hyperlink structure is one of the main results of the analysis, illuminating a) the relative disconnect between local and national hyperlinked agendas, b) the relative disconnect...... between news agendas in the three countries, and c) the connectedness enabled by size, resources and central location in the Scandinavian hyperlinked information structure. The network analysis provides new insights into the relationship between centralized political structures, media ownership dispersal...

  2. Histopathological hoof laminar changes in horses with Pituitary Pars Intermedia Adenoma: cases report

    Directory of Open Access Journals (Sweden)

    L. M. Laskoski

    2015-10-01

    Full Text Available ABSTRACTLaminitis in horses is often associated with endocrine disorders, especially the pituitary pars intermedia dysfunction (PPID in older animals. Morphologic exams of the laminar tissue of the hoof were performed in two horses with suspected PPID, with no clinical signs of laminitis. Changes compatible with laminitis of endocrine origin were observed, such as rounding of the nuclei of the basal cells, thinning and stretching of the secondary epidermal laminae and tissue proliferation. PPID horses with no clinical signs of laminitis may be affected by lesions of the laminar tissue of the hoof that compromise the integrity of the dermal-epidermal junction and may develop clinical symptoms of the disease. It has been suggested that the development stage of endocrine laminitis is longer, but further studies should be conducted to confirm it.

  3. Aislamiento de una cepa de campo de Babesia bigemina (Piroplasma: Babesiidae y establecimiento del cultivo in vitro para la producción de antígenos

    Directory of Open Access Journals (Sweden)

    Roger I Rodríguez-Vivas

    2007-03-01

    Full Text Available La babesiosis bovina ocasionada por Babesia bigemina produce grandes pérdidas a la economía pecuaria Mexicana. Debido a las variaciones antigénicas que presenta B. bigemina es necesario contar con antígenos locales para la realización de estudios epidemiológicos y de inmunización. Se recolectaron 30 garrapatas Boophilus microplus de un bovino adulto clínicamente enfermo de babesiosis (B. bigemina en Yucatán, México. En laboratorio se produjeron larvas, ninfas y una cepa congelada. El aislamiento de la cepa se realizó con éxito y se necesitó 30 días en el cultivo in vitro para obtener un PEI del 1.5 %. La cepa fue congelada en nitrógeno líquido y reactivada en el cultivo celular. La concentración del medio que permitió el mayor PEI (14 %, pIsolation of a field strain of Babesia bigemina (Piroplasma: Babesiidae and establishment of in vitro culture for antigen production. Bovine babesiosis, caused by Babesia bigemina, is a barrier for livestock development; it results in high economic loss to Mexican livestock. Control requires adequate antigens for diagnosis and vaccination programs. However, because of antigenic variation among Babesia strains, it is necessary to use antigens prepared from local strains. The purpose of the present study was to isolate a local field strain and to establish the in vitro culture of B. bigemina by the evaluation of the constituent’s concentration of culture media. Thirty engorged female Boophilus microplus were collected from cattle suffering clinical babesiosis (B. bigemina in Yucatan state, Mexico. These ticks were sent to the laboratory for detection of Babesia sp. vermicules. Eggs were kept at 83-85 % humidity and 27 ºC until hatching. Larvae were transferred to an esplenectomized calf (B-1. The resulting nymphs were transferred to an esplenectomized calf (B-2. Twelve days later, B. bigemina (local strain was detected in calf B-2 and its infected blood was frozen in liquid nitrogen to

  4. Caracterización y purificación parcial de sustancias tipo bacteriocinas producidas por cepas de Enterococcus

    OpenAIRE

    Cardoso, María de las Mercedes Elisa; Cardoso, María de las Mercedes Elisa

    2012-01-01

    Las bacterias ácido lácticas, que gozan del status de “Generalmente Reconocidas Como Seguras”, otorgado por la Organización Mundial de la Salud, pueden producir sustancias antimicrobianas de naturaleza proteica, denominadas bacteriocinas, que actúan como bioconservantes alimentarios. Se investigó la capacidad bacteriocinogénica de la cepa de Enterococcus faecalis DBFIQ E24, aislada de leche cruda producida en la región santafesina. Esta cepa fue sensible a vancomicina y carente de capacidad...

  5. Side effects of Deferasirox Iron Chelation in Patients with Beta Thalassemia Major or Intermedia

    Directory of Open Access Journals (Sweden)

    Murtadha Al-Khabori

    2013-03-01

    Full Text Available Objectives: Chelating agents remain the mainstay in reducing the iron burden and extending patient survival in homozygous beta-thalassemia but adverse and toxic effects may increase with the institution and long term use of this essential therapy. This study aimed to estimate the incidence of deferasirox (DFX side effects in patients with thalassemia major or intermedia.Methods: A retrospective study of 72 patients (mean age: 20.3±0.9 yrs; 36 male, 36 female with thalassemia major or intermedia treated at Sultan Qaboos University Hospital, Oman, was performed to assess the incidence of side effects related to deferasirox over a mean of 16.7 month follow-up period.Results: Six patients experienced rashes and 6 had gastro-intestinal upset. DFX was discontinued in 18 patients for the following reasons: persistent progressive rise(s in serum creatinine (7 patients; 40% mean serum creatinine rise from baseline, feeling unwell (2, severe diarrhea (1, pregnancy (1, death unrelated to chelator (2 and rise in serum transaminases (2. Three patients were reverted to desferoxamine and deferiprone combination therapy as DFX was no longer biochemically effective after 18 months of therapy. There was no correlation between baseline serum ferritin and serum creatinine or a rise in serum creatinine. Cardiac MRI T2* did not change with DFX therapy. However, there was an improvement in liver MRI T2* (p=0.013.Conclusion: Renal side effects related to deferasirox appear to be higher than those reported in published clinical trials. Further larger studies are required to confirm these findings.

  6. Hongos solubilizadores de fosfato en suelo de páramo cultivado con papa (Solanum tuberosum)

    OpenAIRE

    Beltrán Pineda, M. E

    2014-01-01

    Los hongos solubilizadores de fosfato (HSF) son un grupo funcional de microorganismos que juegan un rol fundamental en el ciclaje de fósforo en los suelos; gracias a su actividad, las plantas pueden aprovechar las grandes reservas de fósforo insoluble que se encuentra fijado a los minerales del suelo, contribuyendo a que disminuya la aplicación de fertilizantes fosforados a los suelos. En este estudio se lograron aislar 43 cepas de hongos con capacidad solubilizadora de fosfato a partir de la...

  7. Two cases of urinary tract infection caused by Shiga toxin-producing Escherichia coli 0157:H7 strains Dos casos de infección del tracto urinario causados por cepas de Escherichia coli 0157:H7 productoras de toxina Shiga.

    Directory of Open Access Journals (Sweden)

    María Del P. Gadea

    2012-06-01

    Full Text Available ABSTRACT STEC strains can infect extra-intestinal sites such as the human urinary tract and sometimes cause severe complications. We report two cases of urinary tract infection caused by STEC in two elderly women with comorbidities. Although both strains belonged to the 0157:H7 serotype and carried genes associated with severe illness, none of the patients developed hemolytic uremic syndrome (HUS. These findings provide additional evidence for the presence of these agents in our country and in the region, and highlight the need to maintain an active surveillance system of HUS cases, placing special emphasis on the study of other sites of infection in patients with non-diarrheal HUS.En los seres humanos, las cepas STEC pueden producir infección en sitios extraintestinales, como el tracto urinario, y causar complicaciones graves. Comunicamos dos casos de infección urinaria por STEC en dos mujeres ancianas con comorbilidades. Aunque ambas cepas correspondieron al serotipo 0157:H7 y portaban los genes asociados con enfermedad grave, ninguna de las pacientes desarrolló síndrome urémico hemolítico (SUH. Estos hallazgos constituyen una evidencia adicional de la presencia de estos agentes en nuestro país y en la región, y destacan la necesidad de mantener un sistema activo de vigilancia, con especial énfasis en el estudio de otros sitios de Infección en pacientes que presentan SUH no asociado a diarrea.

  8. Effect of caffeic acid phenethyl ester on Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

    Science.gov (United States)

    Choi, E-Y; Choe, S-H; Hyeon, J-Y; Choi, J-I; Choi, I S; Kim, S-J

    2015-12-01

    Caffeic acid phenethyl ester (CAPE) has numerous potentially beneficial properties, including antioxidant, immunomodulatory and anti-inflammatory activities. However, the effect of CAPE on periodontal disease has not been studied before. This study was designed to investigate the efficacy of CAPE in ameliorating the production of proinflammatory mediators in macrophages activated by lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in periodontal disease. LPS from P. intermedia ATCC 25611 was isolated by using the standard hot phenol-water method. Culture supernatants were assayed for nitric oxide (NO), interleukin (IL)-1β and IL-6. We used real-time polymerase chain reaction to quantify inducible NO synthase, IL-1β, IL-6, heme oxygenase (HO)-1 and suppressors of cytokine signaling (SOCS) 1 mRNA expression. HO-1 protein expression and levels of signaling proteins were assessed by immunoblot analysis. DNA-binding activities of NF-κB subunits were analyzed by using the enzyme-linked immunosorbent assay-based kits. CAPE exerted significant inhibitory effects on P. intermedia LPS-induced production of NO, IL-1β and IL-6 as well as their mRNA expression in RAW264.7 cells. CAPE-induced HO-1 expression in cells activated with P. intermedia LPS, and selective inhibition of HO-1 activity by tin protoporphyrin IX attenuated the inhibitory effect of CAPE on LPS-induced NO production. CAPE did not interfere with IκB-α degradation induced by P. intermedia LPS. Instead, CAPE decreased nuclear translocation of NF-κB p65 and p50 subunits induced with LPS, and lessened LPS-induced p50 binding activity. Further, CAPE showed strong inhibitory effects on LPS-induced signal transducer and activator of transcription 1 and 3 phosphorylation. Besides, CAPE significantly elevated SOCS1 mRNA expression in P. intermedia LPS-stimulated cells. Modulation of host response by CAPE may represent an attractive strategy towards the treatment of periodontal disease

  9. Effect of nitric oxide-releasing derivative of indomethacin on Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

    Science.gov (United States)

    Choe, So-Hui; Choi, Eun-Young; Hyeon, Jin-Yi; Choi, In Soon; Kim, Sung-Jo

    2017-10-14

    The purpose of this study was to investigate the influences of NCX 2121, a nitric oxide (NO)-releasing derivative of indomethacin, upon the generation of proinflammatory mediators using murine macrophages activated by lipopolysaccharide (LPS) isolated from Prevotella intermedia, which is one of the pathogens implicated in periodontal diseases. Inducible NO synthase (iNOS)-derived NO, IL-1β and IL-6 as well as their relevant mRNA were significantly attenuated by NCX 2121 in RAW264.7 cells activated by P. intermedia LPS. NCX 2121 was much more effective than the parental compound indomethacin in reducing these proinflammatory mediators. NCX 2121 triggered induction of heme oxygenase-1 (HO-1) in cells exposed to P. intermedia LPS, and its inhibitory influence upon P. intermedia LPS-elicited NO generation was notably blocked by SnPP treatment. NCX 2121 attenuated NF-κB-dependent SEAP release induced by P. intermedia LPS. NCX 2121 did not display inhibitory action towards IκB-α degradation triggered by LPS. Instead, it significantly diminished nuclear translocation as well as DNA-binding action of NF-κB p50 subunit elicited by P. intermedia LPS. Further, NCX 2121 significantly up-regulated SOCS1 mRNA expression in cells challenged with P. intermedia LPS. In summary, NCX 2121 down-regulates P. intermedia LPS-elicited generation of NO, IL-1β and IL-6 in murine macrophages in a mechanism that involves anti-inflammatory HO-1 induction as well as decrement of NF-κB activation, which may be associated with SOCS1 expression. NCX 2121 may have potential benefits as a host immunomodulatory agent for the therapy of periodontal disease. Copyright © 2017 Elsevier Inc. All rights reserved.

  10. Prevotella intermedia stimulates tissue-type plasminogen activator and plasminogen activator inhibitor-2 expression via multiple signaling pathways in human periodontal ligament cells.

    Science.gov (United States)

    Guan, Su-Min; He, Jian-Jun; Zhang, Ming; Shu, Lei

    2011-06-01

    Prevotella intermedia is an important periodontal pathogen that induces various inflammatory and immune responses. In this study, we investigated the effects of P. intermedia on the plasminogen system in human periodontal ligament (hPDL) cells and explored the signaling pathways involved. Using semi-quantitative reverse transcription (RT)-PCR and quantitative real-time RT-qPCR, we demonstrated that P. intermedia challenge increased tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor (PAI)-2 expression in a concentration- and time-dependent manner, but exerted no influence on urokinase-type plasminogen activator and PAI-1mRNA expression in hPDL cells. Prevotella intermedia stimulation also enhanced tPA protein secretion as confirmed by enzyme-linked immunosorbent assay. Western blot results revealed that P. intermedia treatment increased phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 kinase (p38). ERK, JNK and protein kinase C inhibitors significantly attenuated the P. intermedia-induced tPA and PAI-2 expression. Furthermore, p38 and phosphatidylinositol 3-kinase inhibitors markedly decreased PAI-2 expression, whereas they showed no or little inhibition on tPA expression. In contrast, inhibition of protein kinase A greatly enhanced the upregulatory effect of P. intermedia on tPA and PAI-2 expression. Our results suggest that P. intermedia may contribute to periodontal tissue destruction by upregulating tPA and PAI-2 expression in hPDL cells via multiple signaling pathways. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  11. In vitro antibacterial activity of onion (allium cepa) against clinical isolates of vibrio cholera

    International Nuclear Information System (INIS)

    Hannan, A.; Humayun, T.; Hussain, M.B.; Yasir, M.; Sikandar, S.

    2010-01-01

    Background: Cholera is a major public health problem in developing countries of the world. Bacterial resistance, lack of surveillance data and proper microbiological facilities are major problems regarding diagnosis of cholera. The spread of microbial drug resistance is a global public health challenge that results in increased illness and death rate. Newer antimicrobials or agents are urgently required to overcome this problem. This work was therefore done to investigate the antimicrobial potential of onion against thirty-three clinical isolates of Vibrio cholera. Methods: The extract was prepared by reflux extraction method. Antibacterial screening of clinical isolates of V. cholerae was done by agar well diffusion method. Agar dilution method was used to assess the Minimum Inhibitory Concentration (MIC). Results: All tested strains of V. cholerae were sensitive to onion (Allium cepa) extracts of two types (purple and yellow). Purple type of extract had MIC range of 19.2-21.6 mg/ml. The extract of yellow type onion had an MIC range of 66-68.4 mg/ml. Conclusion: The results indicated that onion (Allium cepa) has an inhibitory effect on V. cholerae. Keeping in view the anti-bacterial activity of this compound can be exploited as a therapeutic agent in an animal model. This finding is a positive point for further investigation of this herb of traditional medicine. (author)

  12. POTASSIUM AND ITS EFFECT ON THE CONTENT OF POLYPHENOLS IN ONION (ALLIUM CEPA L.

    Directory of Open Access Journals (Sweden)

    Petra Kavalcová

    2015-02-01

    Full Text Available Onion (Allium cepa L. is rich of chemoprotective compounds as polyphenols, flavonoids, anthocyanins, vitamins, sulphur compounds which have potential beneficial properties for human health. Potassium as important mineral abundant plays many vital roles in plant nutrition (reduces respiration, activates enzyme. In generally, potassium increases crop yield and improves quality of onion bulbs. The objectives of this work were to compare and evaluate the impact of potassium on the content of total polyphenols and antioxidant activity of onion (Allium cepa L.. The content of the total polyphenols was determined by using the Folin-Ciocalteu reagent (FCR. The absorbance was measured at 765 nm of wave length against blank. Antioxidant activity was measured using a compound DPPH˙ (2.2-diphenyl-1-picrylhydrazyl at 515.6 nm in the spectrophotometer. The content of total polyphenols in samples of onion during vegatation period moved in the range from 505.6 mg GAE/kg ±25.18 to 621.49 mgGAE/kg ±13.41. In this work was watched also the influence of potassium on antioxidant activity, where values were in interval from 32.20 %± 0.58 to 44.67 % ±0.68.

  13. Potential genotoxic effects of melted snow from an urban area revealed by the Allium cepa test.

    Science.gov (United States)

    Blagojević, Jelena; Stamenković, Gorana; Vujosević, Mladen

    2009-09-01

    The presence of well-known atmospheric pollutants is regularly screened for in large towns but knowledge about the effects of mixtures of different pollutants and especially their genotoxic potential is largely missing. Since falling snow collects pollutants from the air, melted snow samples could be suitable for evaluating potential genotoxicity. For this purpose the Allium cepa anaphase-telophase test was used to analyse melted snow samples from Belgrade, the capital city of Serbia. Samples of snow were taken at two sites, characterized by differences in pollution intensity, in three successive years. At the more polluted site the analyses showed a very high degree of both toxicity and genotoxicity in the first year of the study corresponding to the effects of the known mutagen used as the positive control. At the other site the situation was much better but not without warning signals. The results showed that standard analyses for the presence of certain contaminants in the air do not give an accurate picture of the possible consequences of urban air pollution because the genotoxic potential remains hidden. The A. cepa test has been demonstrated to be very convenient for evaluation of air pollution through analyses of melted snow samples.

  14. Hydropower reservoirs: cytotoxic and genotoxic assessment using the Allium cepa root model.

    Science.gov (United States)

    Rambo, Cassiano Lazarotto; Zanotelli, Patrícia; Dalegrave, Daniela; De Nez, Dinara; Szczepanik, Jozimar; Carazek, Fábio; Franscescon, Francini; Rosemberg, Denis Broock; Siebel, Anna Maria; Magro, Jacir Dal

    2017-03-01

    Hydropower offers a reliable source of electricity in several countries, and Brazil supplies its energy needs almost entirely through hydropower plants. Nevertheless, hydropower plants comprise large buildings and water reservoirs and dams, resulting in huge ecological disruptions. Here, we analyzed the impact of four hydropower reservoirs construction in metals and pesticides incidence and the cytotoxic and genotoxic potential of sediment elutriate of rivers from southern Brazil. Our analyses have evidenced the elevated incidence of different metals (lead, iron, cadmium, and chrome) and pesticides (methyl parathion, atrazine, and 2,4-dichlorophenoxyacetic acid). We showed that Allium cepa exposed to sediment elutriates did not change the seed germination rate and mitotic index. However, roots from Allium cepa exposed to reservoirs sediment elutriates showed increased occurrence of chromosomal aberrations and nuclear abnormalities. Therefore, the results obtained in our study indicate that sediment from reservoirs present elevated concentration of metals and pesticides and a significant genotoxic potential. Taken together, our data support that hydropower reservoirs represent an environmental scenario that could impact surrounding wildlife and population.

  15. Estabelecimento de colonia, em laboratório, de Lutzomyia intermedia Lutz & Neiva, 1912 (Diptera, Psychodidae, Phlebotominae Establishment of colony, in laboratory, of Lutzomyia intermedia Lutz & Neiva, 1912 (Diptera, Psychodidae, Phlebotominae

    Directory of Open Access Journals (Sweden)

    Elizabeth F. Rangel

    1985-06-01

    Full Text Available Para a utilização em infecções experimentais e xenodiagnósticos de infecções naturais por leishmânias dermotrópicas do Rio de Janeiro, estabelecemos, em laboratório, uma colônia de Lutzomyia intermedia apresentando aqui a metodologia seguida, juntamente com dados relativos ao rendimento e duração de cada fase evolutiva nas quatro primeiras gerações.A laboratory colony of the sandfly Lutzomyia intermedia was established (at present in its seventh generation in order to provide specimens for experimental infection and for experimental xenodiagnosis of natural infections due to cutaneous leishmanias in Rio de Janeiro. Date are presented on the methodology and on the yield and duration of each stage of development, during the first four generations.

  16. Degradación microbiana de cianuro procedente de plantas de beneficio de oro mediante una cepa nativa de P. fluorecens

    Directory of Open Access Journals (Sweden)

    OSCAR JAIME RESTREPO

    2006-01-01

    Full Text Available El cianuro es el compuesto lixiviante más utilizado en la industria metalúrgica de metales preciosos. El cianuro presenta efectos nocivos, entre los que se destacan su alta toxicidad y su efecto inhibitorio en la respiración celular. En este trabajo se probó la alternativa biotecnológica de degradar cianuro empleando una cepa nativa de P. fluorescens, aislada a partir de muestras de arenas procedentes de plantas de beneficio de oro que utilizan la cianuración ubicadas en municipio de Segovia, Colombia. En la mayor parte de las plantas de tratamiento no se controlan técnicamente los procesos, lo cual lleva a la utilización de cantidades de NaCN por encima de las necesarias, generando altas descargas del exceso tanto en solución como en las arenas descartadas. Las pruebas de toxicidad realizadas con D. pulex indican que una concentración de cianuro mayor o igual a 54 ppm es suficiente para intoxicar e inhibir su sobrevivencia. Los tratamientos convencionales para degradación de cianuro, suelen ser muy comunes y eficientes, sin embargo presentan desventajas por el alto costo que conlleva la compra de los reactivos necesarios. El tratamiento microbiano por su parte, demuestra eliminar grandes cantidades de cianuro de una manera eficiente y económica.

  17. Serogrupos y susceptibilidad antimicrobiana en cepas de Shigella Serogroups and antimicrobial susceptibility in Shigella strains

    Directory of Open Access Journals (Sweden)

    Leonor Díaz Rigau

    2009-03-01

    Full Text Available INTRODUCCIÓN. Shigella spp. es uno de los agentes causales más importantes de diarrea aguda en los niños. La presente investigación tuvo como objetivo conocer la frecuencia de serogrupos y la susceptibilidad antimicrobiana a los fármacos de elección y a los alternativos. MÉTODOS. Se realizó un estudio descriptivo y retrospectivo entre enero de 2004 y diciembre de 2006 a partir de 34 cepas de Shigella spp. aisladas en heces de niños menores de 5 años ingresados en el Hospital «Aleida Fernández Chardiet» (Municipio Güines a causa de enfermedad diarreica aguda. RESULTADOS. Los serogrupos encontrados fueron S. sonnei (70,5 % y S. flexneri (29,5 %. Ambos serogrupos mostraron altos niveles de resistencia al trimetoprim-sulfametoxazol y a la ampicilina, además en las cepas de S. sonnei se encontró resistencia al ácido nalidíxico y en las de S. flexneri al cloranfenicol. Todas las cepas mostraron altos porcentajes de sensibilidad a la ceftriaxona, norfloxacina y ciprofloxacina. El 70 % de las cepas de S. sonnei fueron multirresistentes. El patrón de multirresistencia (ampicilina, trimetoprim-sulfamtetoxazol y ácido nalidíxico se encontró en ambos serogrupos. CONCLUSIONES. La determinación y vigilancia de los patrones de resistencia facilita el control de la política de uso de antibióticos en la región estudiada y previene el surgimiento de cepas resistentes a fármacos de nueva generación.INTRODUCTION: Shigella ssp. is one of the more important causal agents of acute diarrhea in children. Present research has as aim to know serogroups frequency and antimicrobial susceptibility to choice drugs, and to its alternatives. METHODS: A descriptive retrospective study was carried out between January 2004 and December 2006 of 34 strains of Shigella isolated from children lower than 5 years admitted in "Aleida Fernández Chardiet" Hospital in Güines Municipality by acute diarrheic disease. RESULTS: Serogroups included S. sonnei (70

  18. Raíz secundaria embrional asociada a la germinación de Garcinia intermedia (Clusiaceae y su posible papel en la supervivencia de las plántulas

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    José Fco Di Stefano

    2006-09-01

    Full Text Available En pruebas preliminares de germinación con semillas de Garcinia intermedia (Clusiaceae se observó que ésta producía dos tipos de raíces: la primaria y una secundaria embrional que atraviesa longitudinalmente la semilla. Para determinar su posible papel en la supervivencia y crecimiento de esta especie, se analizaron 90 plántulas con al menos seis meses de germinadas en bolsas plásticas con suelo orgánico ubicadas en un invernadero, a las que se les aplicó uno de los siguientes tratamientos: corte de la raíz primaria o la secundaria embrional, además de un grupo control con ambas raíces (30 repeticiones de cada uno. Luego de tres meses se empezaron a extraer 10 plántulas/mes/tratamiento a los que se les midió la altura total, diámetro basal, longitud de la raíz primaria y secundaria, y la biomasa del vástago, raíz y semilla sin la cubierta seminal. Las plántulas que obtuvieron el mayor crecimiento fueron las del control, seguidas por aquéllas sin la raíz secundaria. Sin embargo, más del 90% de las plántulas a las que se les cortó la raíz primaria, sobrevivieron después de cinco meses de haberse aplicado el tratamiento, en parte debido a una muy alta capacidad de recuperación de su sistema radical, ya sea produciendo rebrotes de la principal (20%, aumentando el crecimiento de la raíz secundaria (13.3%, o ambos (26.7%, y a las reservas de la semilla. La longitud de los rebrotes de la raíz principal fue estadísticamente significativa en las primeras dos extracciones mensuales de plantas, comparado con las del control (F6= 18.6, F7= 16.0, pSecondary embryonary root associated with seed germination in Garcinia intermedia (Clusiaceae and its possible role in seedling survival. Germination tests on Garcinia intermedia (Clusiaceae seeds showed the growth of two types of roots: additionally to the primary root, a secondary root crosses the seed lengthwise. To determine its possible role on the survival and growth of this

  19. Disponibilidade hídrica e germinação de sementes de cebola (Allium cepa L. Water availability and germination of onion seeds (Allium cepa L.

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    Z. Piana

    1994-12-01

    Full Text Available Objetivando verificar a influência do teor de água do substrato na germinação de sementes de cebola (Allium cepa L., conduziu-se um ensaio sob condições de ambiente de laboratório. Os efeitos de cinco níveis de água no substrato solo x areia (3:1, 20, 40, 60, 80 e 100% da capacidade de retenção foram avaliados através da porcentagem de emergência no 14° dia da semeadura e pesos da matéria verde e seca das plântulas normais. Para tanto, 200 sementes do cultivar Pira Ouro por tratamento, em quatro repetições de 50, foram semeadas a 1,5 cm de profundidade, mantendo-se constantes os níveis de água no substrato. Os resultados possibilitaram concluir que os níveis de umidade do solo indicados para germinação de sementes de cebola são de 40% e 60% da capacidade de retenção do substrato, por possibilitarem uma melhor emergência, peso da matéria verde e seca das plântulas.One essay was conducted under laboratory conditions, in order to verify the influence of substrate water content on the germination of onion (Allium cepa L. seeds. The effect of five water levels in the medium soil x sand (3:1 - 20, 40, 60, 80 and 100% retention capacity-were evaluated through the emergence percentage 14 days after seedling and the weight of fresh and dry matters of normal seedlings. For that purpose, 200 treated seeds of Pira Ouro cultivar were sown 1.5 cm deep in four replicátions of 50, with a constant water content in the medium. The best results showed that the soil moisture levels suggested for the germination of onion seeds were 40% to 60% of the substrate retention capacity, once they provide better emergence, weight of the fresh and dry matters of seedlings.

  20. La periferia espontánea en las ciudades intermedias latinoamericanas: perspectivas de solución desde la dimensión territorial-ambiental de la sostenibilidad

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    José Jorge Peralta Arias

    2017-05-01

    Full Text Available La investigación aborda el problema de la insostenibilidad de la periferia espontánea en las ciudades intermedias latinoamericanas, cuya informalidad ha determinado un crecimiento discontinuo, una baja densidad y el déficit de infraestructuras y dotaciones; a esto, se suman los riesgos futuros por desastres de origen natural asociados al cambio climático. En dicho contexto, el trabajo se plantea como objetivo central contribuir a solucionar este problema, a partir de una propuesta metodológica para el estudio de las periferias espontáneas de las ciudades latinoamericanas de escala intermedia, desde la perspectiva del desarrollo sostenible, poniendo énfasis en la dimensión física, ambiental y climática. Para ello, la investigación se organiza en tres partes: revisión y análisis de la información existente sobre la temática y consulta a expertos, para la determinación de criterios; la determinación de acciones prioritarias; y una propuesta de estructura que sustenta un sistema de indicadores. Los resultados del trabajo proponen una batería de treinta y nueve (39 indicadores referenciales, articulados en cuatro subsistemas relacionados con: la vulnerabilidad física-ambiental y climática, el eje ambiental, el desarrollo urbano y la cohesión social. Estos constituyen la principal conclusión del trabajo, el que, se espera, será útil para comenzar a tomar las primeras decisiones frente al problema de este tipo de periferias.

  1. The production of 7-methyljuglone, plumbagin and quercetin in wild and cultivated Drosera rotundifolia and Drosera intermedia

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    B. Baranyai

    2016-09-01

    Full Text Available The recent establishment of Sphagnum farming areas has created large artificial habitats where Drosera grows under semi-natural conditions. Here we test the suitability, for pharmaceutical purposes, of two Drosera species collected from such areas. We measured the concentration of the biologically active compounds 7-methyljuglone, plumbagin and quercetin in Drosera rotundifolia and D. intermedia. All three compounds were found in pharmacologically suitable concentrations with 7-methlyjuglone characteristic for D. rotundifolia and plumbagin for D. intermedia. The concentrations required for pharmacological purposes were achieved within one year, but higher concentrations occurred in older plants and plants in flower. Concentrations did not differ between plants collected in the morning and in the afternoon. Drosera plants cultivated under semi-natural conditions are suitable as sources of raw materials for industrial pharmacological applications.

  2. Evaluation of antioxidant potential of Lavandula x intermedia Emeric ex Loisel. 'Budrovka': a comparative study with L. angustifolia Mill.

    Science.gov (United States)

    Blazeković, Biljana; Vladimir-Knezević, Sanda; Brantner, Adelheid; Stefan, Maja Bival

    2010-08-30

    A Croatian indigenous cultivar of lavandin, Lavandula x intermedia 'Budrovka' (Lamiaceae) was studied for the phenolic acids, flavonoids, anthocyanins, procyanidins and total tannins, as well as total polyphenols content, in the flower, inflorescence stalk and leaf ethanolic extracts. Antioxidant potentials on these plant part extracts were assessed by the DPPH free radical scavenging activity, iron chelating activity, reducing power, lipid peroxidation inhibition properties and total antioxidant capacity assays. All results were compared with those of Lavandula angustifolia, the only member of the Lavandula genus officially used in modern phytotherapy. Based on the results of our parallel study, we may suggest that Lavandula x intermedia 'Budrovka' is as potent an antioxidant as Lavandula angustifolia and the antioxidant activity of the Lavandula extracts is mainly due to the presence of rosmarinic acid. A good correlation was found between the polyphenol contents and antioxidant activities of the extracts.

  3. Evaluation of Antioxidant Potential of Lavandula x intermedia Emeric ex Loisel. 'Budrovka': A Comparative Study with L. angustifolia Mill.

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    Maja Bival Štefan

    2010-08-01

    Full Text Available A Croatian indigenous cultivar of lavandin, Lavandula x intermedia 'Budrovka' (Lamiaceae was studied for the phenolic acids, flavonoids, anthocyanins, procyanidins and total tannins, as well as total polyphenols content, in the flower, inflorescence stalk and leaf ethanolic extracts. Antioxidant potentials on these plant part extracts were assessed by the DPPH free radical scavenging activity, iron chelating activity, reducing power, lipid peroxidation inhibition properties and total antioxidant capacity assays. All results were compared with those of Lavandula angustifolia, the only member of the Lavandula genus officially used in modern phytotherapy. Based on the results of our parallel study, we may suggest that Lavandula x intermedia 'Budrovka' is as potent an antioxidant as Lavandula angustifolia and the antioxidant activity of the Lavandula extracts is mainly due to the presence of rosmarinic acid. A good correlation was found between the polyphenol contents and antioxidant activities of the extracts

  4. Organización y manejo de la colección de cepas de referencia del Instituto Finlay

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    Elsie Iglesias

    2009-04-01

    Full Text Available La Colección de Cultivos Microbianos del Instituto Finlay está constituida por cepas para la producción, la investigación y desarrollo de vacunas, así como de referencia para el control de la calidad de medios de cultivo, desinfectantes e higienizantes, entre otros. Mientras que la metodología para el manejo de los lotes de siembra de referencia de las cepas vacunales se encuentra adecuadamente establecida, en el caso de las cepas de referencia, la colección no se encontraba en óptimas condiciones. Por este motivo se elaboraron nuevos lotes de referencia a partir de cepas obtenidas de colecciones reconocidas y se estableció la documentación adecuada que incluye: expedientes de cada lote, los procedimientos normalizados de operación, los registros de elaboración y control de los lotes, las especificaciones de calidad y los certificados de ensayo.

  5. Potencial probiótico de cepas nativas para uso como aditivos en la alimentación avícola

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    Cecilia Lara Mantilla

    2012-01-01

    Full Text Available Título en ingles: Probiotic potential of native strains, for as feed additives for poultry Resumen: Se evaluó in vitro el potencial probiótico de cepas nativas aisladas de las heces de pollos asilvestrados (Gallus gallus pertenecientes a los géneros Lactobacillus sp, Bacillus sp y levaduras tipo saccharomyces sp; se determinó la actividad probiótica mediante pruebas de resistencia al ácido (pH  3, 4, 5, 6, 7, sales de bilis (0,05, 0,1, 0,15, 0.3 %, tolerancia al NaCl (2, 4, 7, 10 %, actividad antagónica (Salmonella sp, E. coli, determinación del tipo de fermentación, crecimiento a temperaturas (28,37,43°C y capacidad de crecimiento. Las cepas con mayor tolerancia se identificaron a través de pruebas bioquímicas y fermentación de carbohidratos. Como resultado se observó que tres microorganismos: Saccharomyces sp. (3, Bacillus sp. (7 y Lactobacillus sp. (14 poseen propiedades probióticas. Palabras claves: Gallus gallus; Saccharomyces sp.;  Bacillus sp.; Lactobacillus sp.; aditivos  microbianos. Abstract: Was evaluated in vitro the probiotic potential of native strains isolated from feces of wild chickens (Gallus gallus belonging to the genera Lactobacillus sp., Bacillus sp. and Saccharomyces ; probiotic activity was determined by testing acid resistance (pH 3, 4, 5.6, 7, bile salts (0,05, 0,1, 0,15, 0,3%, tolerance to NaCl (2, 4, 7,10%, antagonistic activity (Salmonella spp, E. coli, production gas (glucose, growth temperatures (28, 37, 43 ° C and growth capacity. The most tolerant strains were identified by biochemical tests and carbohydrate fermentation. As a result it was found that three microorganisms: Saccharomyces sp. (3, Bacillus sp. (7 and Lactobacillus sp. (14 have probiotic properties. Key words: Gallus gallus; Saccharomyces sp.;  Bacillus sp.; Lactobacillus sp.; microbial additives.

  6. Evaluation of Antioxidant Potential of Lavandula x intermedia Emeric ex Loisel. 'Budrovka': A Comparative Study with L. angustifolia Mill.

    OpenAIRE

    Blažeković, Biljana; Vladimir-Knežević, Sanda; Brantner, Adelheid; Štefan, Maja Bival

    2010-01-01

    A Croatian indigenous cultivar of lavandin, Lavandula x intermedia 'Budrovka' (Lamiaceae) was studied for the phenolic acids, flavonoids, anthocyanins, procyanidins and total tannins, as well as total polyphenols content, in the flower, inflorescence stalk and leaf ethanolic extracts. Antioxidant potentials on these plant part extracts were assessed by the DPPH free radical scavenging activity, iron chelating activity, reducing power, lipid peroxidation inhibition properties and total antioxi...

  7. Curcumin suppresses the production of interleukin-6 in Prevotella intermedia lipopolysaccharide-activated RAW 264.7 cells

    Science.gov (United States)

    2011-01-01

    Purpose Curcumin is known to exert numerous biological effects including anti-inflammatory activity. In this study, we investigated the effects of curcumin on the production of interleukin-6 (IL-6) by murine macrophage-like RAW 264.7 cells stimulated with lipopolysaccharide (LPS) from Prevotella intermedia, a major cause of inflammatory periodontal disease, and sought to determine the underlying mechanisms of action. Methods LPS was prepared from lyophilized P. intermedia ATCC 25611 cells by the standard hot phenol-water method. Culture supernatants were collected and assayed for IL-6. We used real-time polymerase chain reaction to detect IL-6 mRNA expression. IκB-α degradation, nuclear translocation of NF-κB subunits, and STAT1 phosphorylation were characterized via immunoblotting. DNA-binding of NF-κB was also analyzed. Results Curcumin strongly suppressed the production of IL-6 at both gene transcription and translation levels in P. intermedia LPS-activated RAW 264.7 cells. Curcumin did not inhibit the degradation of IκB-α induced by P. intermedia LPS. Curcumin blocked NF-κB signaling through the inhibition of nuclear translocation of NF-κB p50 subunit. Curcumin also attenuated DNA binding activity of p50 and p65 subunits and suppressed STAT1 phosphorylation. Conclusions Although further study is required to explore the detailed mechanism of action, curcumin may contribute to blockade of the host-destructive processes mediated by IL-6 and appears to have potential therapeutic values in the treatment of inflammatory periodontal disease. PMID:21811692

  8. Kinetics activity of Yersinia Intermedia Against ZnO Nanoparticles Either Synergism Antibiotics by Double-Disc Synergy Test Method.

    Science.gov (United States)

    Fathi Azar Khavarani, Motahareh; Najafi, Mahla; Shakibapour, Zahra; Zaeifi, Davood

    2016-03-01

    Bacterial resistance to the commonly used antibacterial agents is an increasing challenge in the medicine, and a major problem for the health care systems; the control of their spread is a constant challenge for the hospitals. In this study, we have investigated the antimicrobial activity of the Zinc Oxide nanoparticles against clinical sample; Yersinia intermedia bacteria. Nanoparticle susceptibility constants and death kinetic were used to evaluate the antimicrobial characteristics of the Zinc Oxide (ZnO) against the bacteria. Antimicrobial tests were performed with 10 8 cfu.mL -1 at baseline. At first, Minimum Inhibitory Concentration (MIC) of ZnO was determined and then nanoparticle suspension at one and two times of the MIC was used for death kinetic and susceptibility constant assay at 0 to 360 min treatment time. ZnO nanoparticles with size ranging from 10 to 30 nm showed the highest susceptibility reaction against Y. intermedia (Z=39.06 mL.μg -1 ). The process of Y. intermedia death in ZnO suspension was assumed to follow the first-order kinetics and the survival ratio of bacteria decreased with the increasing treatment time. An increased concentration of the nanoparticle was seen to enhance the bactericidal action of the nanoparticle. Then we performed the best ratio of the nanoparticles on semi-sensitive and resistance antibiotic for the bacteria. However, based on experimental results, synergy of ZnO nanoparticles and Oxacilin was determined and Y. intermedia showed a higher sensitivity compared to the ZnO nanoparticles alone. The results of the present study illustrates that ZnO has a strong antimicrobial effect and could potentially be employed to aid the bacterial control. It could also improve- antibacterial effects in combination with the antibiotics.

  9. Detección de cepas de Neisseria meningitidis resistentes a rifampicina en el Uruguay Detection of rifampicin-resistant strains of Neisseria meningitidis in Uruguay

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    Gabriel Pérez Giffoni

    2011-12-01

    Full Text Available El objetivo de este trabajo fue caracterizar fenotípica y genotípicamente dos aislamientos de Neisseria meningitidis resistentes a rifampicina relacionados con dos eventos independientes de transmisión de enfermedad meningocócica grave que se presentaron en septiembre y octubre de 2010 en Montevideo, Uruguay. Se revisó también la base de datos de la vigilancia nacional de resistencia a los antimicrobianos de los últimos 10 años, para estimar la frecuencia de la particularidad de los meningococos caracterizados. La resistencia a rifampicina se estudió por el método epsilométrico. El serotipo y serosubtipo de los aislamientos se determinaron por ELISA y la caracterización genotípica se realizó por digestión del ADN con NheI y electroforesis en gel con campo pulsátil. Ambos aislamientos eran idénticos, B:2a:P1.5, y su fenotipo no figuraba en la colección de 408 cepas de N. meningitidis aisladas en el Uruguay en los últimos 10 años, con la excepción de dos aislamientos sensibles a rifampicina. Los dos aislamientos estudiados también compartían un pulsotipo único, diferente del de otros dos aislamientos resistentes a rifampicina obtenidos en 2003 y 2007. Por lo tanto, ambos eventos de transmisión fueron causados por una única cepa resistente a rifampicina, que podría haberse introducido al país desde otras regiones o haberse originado por un cambio del serogrupo C al B, como producto de la presión selectiva ejercida por vacunas administradas a la población. Es necesario mantener y extremar la vigilancia. No obstante, en vista de que hasta el momento este tipo de hallazgo ha sido esporádico, no se justifica cambiar el fármaco antimicrobiano que se administra a los contactos para la profilaxis, a menos que se identifique un caso secundario.The objective of this study was to characterize the phenotype and genotype of two isolates of rifampicin-resistant Neisseria meningitidis associated with two independent events

  10. Influence of in-feed zinc bacitracin and tiamulin treatment on experimental avian intestinal spirochaetosis caused by Brachyspira intermedia.

    Science.gov (United States)

    Hampson, D J; Oxberry, S L; Stephens, C P

    2002-06-01

    Thirty individually caged layer hens were inoculated with Brachyspira intermedia, and 20 control birds remained unchallenged. Birds received a diet containing 100 parts/10(6) zinc bacitracin (ZnB), and were monitored for 10 weeks. B. intermedia was recovered sporadically from five of the inoculated birds, and there were no significant effects on body weight, faecal water or egg production. ZnB was presumed to be indirectly inhibiting spirochaete growth, and when removed from the diet, 18 of the 30 inoculated birds rapidly became culture positive. After 4 weeks, 10 of the 30 infected birds were treated with tiamulin at 25 mg/kg for 5 days, and 10 were returned to the diet containing ZnB. Birds receiving tiamulin became spirochaete negative and maintained their egg production, but re-infection occurred. The other 20 infected birds had a significant drop in egg production, but those receiving ZnB showed a reduced colonization by B. intermedia after 3 weeks.

  11. Fatty acid methyl ester from Neurospora intermedia N-1 isolated from Indonesian red peanut cake (oncom merah).

    Science.gov (United States)

    Priatni, S; Hartati, S; Dewi, P; Kardono, L B S; Singgih, M; Gusdinar, T

    2010-08-01

    The objective of this study was to identify the Fatty Acid Methyl Ester (FAME) from Neurospora intermedia N-1 that isolated from Indonesian red peanut cake (oncom). FAME profiles have been used as biochemical characters to study many different groups of organisms, such as bacteria and yeasts. FAME from N. intermedia N-1 was obtained by some stages of extraction the orange spores and fractination using a chromatotron. The pure compound (1) was characterized by 500 mHz NMR (1H and 13C), FTIR and LC-MS. Summarized data's of 1H and 13C NMR spectra of compound 1 contained 19 Carbon, 34 Hydrogen and 2 Oxygen (C19H34O2). The position of the double bonds at carbon number 8 and 12 were indicated in the HMBC spectrum (2D-NMR). LC-MS spectrum indicates molecular weight of the compound 1 as 294 which is visible by the presence of protonated molecular ion [M+H] at m/z 295. Methyl esters of long chain fatty acids was presented by a 3 band pattern of IR spectrum with bands near 1249, 1199 and 1172 cm(-1). We suggested that the structure of the pure compound 1 is methyl octadeca-8,12-dienoate. The presence methyl octadeca-8,12-dienoate in N. intermedia is the first report.

  12. Human dental stem cells suppress PMN activity after infection with the periodontopathogens Prevotella intermedia and Tannerella forsythia

    Science.gov (United States)

    Hieke, Cathleen; Kriebel, Katja; Engelmann, Robby; Müller-Hilke, Brigitte; Lang, Hermann; Kreikemeyer, Bernd

    2016-01-01

    Periodontitis is characterized by inflammation associated with the colonization of different oral pathogens. We here aimed to investigate how bacteria and host cells shape their environment in order to limit inflammation and tissue damage in the presence of the pathogen. Human dental follicle stem cells (hDFSCs) were co-cultured with gram-negative P. intermedia and T. forsythia and were quantified for adherence and internalization as well as migration and interleukin secretion. To delineate hDFSC-specific effects, gingival epithelial cells (Ca9-22) were used as controls. Direct effects of hDFSCs on neutrophils (PMN) after interaction with bacteria were analyzed via chemotactic attraction, phagocytic activity and NET formation. We show that P. intermedia and T. forsythia adhere to and internalize into hDFSCs. This infection decreased the migratory capacity of the hDFSCs by 50%, did not disturb hDFSC differentiation potential and provoked an increase in IL-6 and IL-8 secretion while leaving IL-10 levels unaltered. These environmental modulations correlated with reduced PMN chemotaxis, phagocytic activity and NET formation. Our results suggest that P. intermedia and T. forsythia infected hDFSCs maintain their stem cell functionality, reduce PMN-induced tissue and bone degradation via suppression of PMN-activity, and at the same time allow for the survival of the oral pathogens. PMID:27974831

  13. Biocidal Potential and Chemical Composition of Industrial Essential Oils from Hyssopus officinalis, Lavandula × intermedia var. Super, and Santolina chamaecyparissus.

    Science.gov (United States)

    Ortiz de Elguea-Culebras, Gonzalo; Sánchez-Vioque, Raúl; Berruga, María Isabel; Herraiz-Peñalver, David; González-Coloma, Azucena; Andrés, María Fé; Santana-Méridas, Omar

    2018-01-01

    This work presents the biocidal (insecticidal, ixodicidal, nematicidal, and phytotoxic) effects and chemical compositions of three essential oils obtained from the industrial steam distillation (IEOs) of hyssop (Hyssopus officinalis L.), lavandin (Lavandula × intermedia or L. × hybrida var. Super), and cotton lavender (Santolina chamaecyparissus L.). Their chemical composition analyzed by gas chromatography coupled to mass spectrometry showed 1,8-cineole (53%) and β-pinene (16%) as the major components of H. officinalis, linalyl acetate (38%) and linalool (29%) of L. × intermedia; and 1,8-cineole (10%) and 8-methylene-3-oxatricyclo[5.2.0.0 2,4 ]nonane (8%) in S. chamaecyparissus. The biocidal tests showed that L. × intermedia IEO was the most active against the insect Spodoptera littoralis and toxic to the tick Hyalomma lusitanicum, IEO of H. officinalis was strongly active against S. littoralis, and finally, S. chamaecyparissus IEO was a strong antifeedant against the aphid Rhopalosiphum padi, toxic to H. lusitanicum and with moderate effects against Leptinotarsa decemlineata, S. littoralis, and Lolium perenne. © 2018 Wiley-VHCA AG, Zurich, Switzerland.

  14. Prevotella intermedia induces severe bacteremic pneumococcal pneumonia in mice with upregulated platelet-activating factor receptor expression.

    Science.gov (United States)

    Nagaoka, Kentaro; Yanagihara, Katsunori; Morinaga, Yoshitomo; Nakamura, Shigeki; Harada, Tatsuhiko; Hasegawa, Hiroo; Izumikawa, Koichi; Ishimatsu, Yuji; Kakeya, Hiroshi; Nishimura, Masaharu; Kohno, Shigeru

    2014-02-01

    Streptococcus pneumoniae is the leading cause of respiratory infection worldwide. Although oral hygiene has been considered a risk factor for developing pneumonia, the relationship between oral bacteria and pneumococcal infection is unknown. In this study, we examined the synergic effects of Prevotella intermedia, a major periodontopathic bacterium, on pneumococcal pneumonia. The synergic effects of the supernatant of P. intermedia (PiSup) on pneumococcal pneumonia were investigated in mice, and the stimulation of pneumococcal adhesion to human alveolar (A549) cells by PiSup was assessed. The effects of PiSup on platelet-activating factor receptor (PAFR) transcript levels in vitro and in vivo were analyzed by quantitative real-time PCR, and the differences between the effects of pneumococcal infection induced by various periodontopathic bacterial species were verified in mice. Mice inoculated with S. pneumoniae plus PiSup exhibited a significantly lower survival rate, higher bacterial loads in the lungs, spleen, and blood, and higher inflammatory cytokine levels in the bronchoalveolar lavage fluid (macrophage inflammatory protein 2 and tumor necrosis factor alpha) than those infected without PiSup. In A549 cells, PiSup increased pneumococcal adhesion and PAFR transcript levels. PiSup also increased lung PAFR transcript levels in mice. Similar effects were not observed in the supernatants of Porphyromonas gingivalis or Fusobacterium nucleatum. Thus, P. intermedia has the potential to induce severe bacteremic pneumococcal pneumonia with enhanced pneumococcal adhesion to lower airway cells.

  15. [Effects of cutting and reseeding on the ground-dwelling arthropod community in Caragana intermedia forest in desert steppe].

    Science.gov (United States)

    Liu, Ren-Tao; Chai, Yong-Qing; Yang, Xin-Guo; Song, Nai-Ping; Wang, Xin-Yun; Wang, Lei

    2013-01-01

    Taking a 25-year-old Caragana intermedia forest in desert steppe as test object, an investigation was conducted on the ground-dwelling arthropod community in cutting and no-cutting stands with and without reseeding, aimed to understand the effects of cutting, reseeding and their interaction on the individual number and group richness of ground-dwelling arthropod in C. intermedia forest. There were significantly lower number and richness of ground-dwelling arthropod in the open spaces than under the shrubs in the no-cutting and no-reseeding stands. Cutting, reseeding and both of them could significantly increase the number and richness of ground-dwelling arthropod in the open spaces, but not under the shrubs, compared with no cutting or reseeding. Consequently, there were no significant differences in the distribution of ground-dwelling arthropod in the open spaces and under the shrubs in the cutting, reseeding, or cutting and reseeding stands. Further, there was a similar buffer effect between cutting and reseeding on the ground-dwelling arthropod. No significant differences were observed in the ground-dwelling arthropod distribution, between cutting stand and reseeding stand, between cutting stand and cutting and reseeding stand, and between reseeding stand and cutting and reseeding stand. It was suggested that cutting, reseeding, or both of them could significantly improve the ground-dwelling arthropod diversity especially in the open spaces, being beneficial for the restoration of degraded grassland ecosystem and the rational management on artificial C. intermedia forest in desert steppe.

  16. Distribución de grupos filogenéticos y factores de virulencia en cepas de Escherichia coli uropatógena productora de ß-lactamasa CTX-M-15 aisladas de pacientes de la comunidad en Mérida, Venezuela

    Directory of Open Access Journals (Sweden)

    Ysheth Millán

    Full Text Available En este estudio se determinó el perfil de distribución de grupos filogéneticos y la detección genética de factores de virulencia en cepas de Escherichia coli uropatógena (ECUP productoras de ß-lactamasa CTX-M-15. Veintiocho cepas fueron aisladas de pacientes con infección del tracto urinario (ITU que asistieron al Laboratorio de Salud Pública del estado Mérida, Venezuela, durante el lapso comprendido entre enero 2009 y julio 2011. La determinación de los grupos filogenéticos y la detección de seis genes de virulencia, fimH, fyuA, kpsMTII, usp, PAI y papAH, se realizó mediante amplificación por PCR. Quince cepas de 28 se ubicaron principalmente en el filogrupo A, seguidos por el B2 (12/28 y D (1/28. No se observó una relación directa entre la recurrencia o gravedad de la ITU y la distribución de los filogrupos. Todos los factores de virulencia estudiados se encontraron con la frecuencia más alta en el grupo B2. El perfil de virulencia prevalente estuvo conformado por la asociación de tres genes principales: fimH, fyuA y kpsMTII y en menor frecuencia, por la presencia de otros determinantes como usp, PAI y/o papAH. Estos resultados indican que la mayoría de ECUP estuvieron dotadas de tres propiedades virulentas importantes: adhesión, captación de hierro y evasión de la fagocitosis, las cuales favorecieron la producción de ITU recurrentes. Este es el primer trabajo que describe la asociación de grupos filogenéticos con el potencial de virulencia de cepas de ECUP productoras de ß-lactamasa CTX-M-15 en Venezuela.

  17. Immunoglobulin Fc receptors in clinical strains of Staphylococcus aureus do not confer resistance to Phagocytosis in an in vitro assay Los receptores Fc para inmunoglobulinas en cepas clínicas de Staphylococcus aureus no confieren resistencia a la fagocitosis in vitro

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    Benito VEGA

    1999-05-01

    Full Text Available Staphylococcus aureus binds Immunoglobulin G (IgG on its external surface due to the presence of specific receptors for the Fc domain of this immunoglobulin. This mechanism represents a kind of camouflage against phagocytic cells. In order to confirm that possibility an in vitro evaluation of the phagocytic activity of leukocytes polymorpho-nuclear (PMN against strains of Staphylococcus aureus was done, comparing 18 strains isolated from clinical samples and 16 from healthy individuals. The presence of Fc receptors was evaluated by haemagglutination (HA with erythrocytes group A after incubation of the strains with IgG anti blood group A. Phagocytosis of S. aureus was carried out by mixing live bacteria with a suspension of human PMN and incubating at 37 °C for 1 h; survivors were counted as colony forming units by plating. The strains from clinical specimens showed higher HA than those from healthy individuals (p = 0.01; but the former were killed more efficiently than the latter (80-90% and 40%, respectively. It is may be possible that S. aureus showed different behavior in vivo, where could express other virulence factors to prevent the action of phagocytes.Staphylococcus aureus liga inmunoglobulinas G (IgG a su superficie externa debido a la presencia de receptores para el dominio Fc de esas inmunoglobulinas. Este mecanismo representa una clase de camuflage contra células fagocíticas. Para confirmar tal posibilidad se realizó una evaluación in vitro de la actividad fagocítica de leucocitos polimorfonucleares (PMN contra cepas de Staphylococcus aureus, comparando 18 cepas aisladas de casos clínicos y 16 de individuos sanos. La presencia de receptores fue evaluada por hemaglutinación (HA con eritrocitos grupo A luego que las cepas fueron incubadas con IgG anti grupo sanguíneo A. La fagocitosis de S. aureus fue realizada mezclando células vivas con una suspensión de PMN e incubada a 37 °C por una hora; las bacterias sobrevivientes

  18. Efficacy assessment of acid mine drainage treatment with coal mining waste using Allium cepa L. as a bioindicator.

    Science.gov (United States)

    Geremias, Reginaldo; Bortolotto, Tiago; Wilhelm-Filho, Danilo; Pedrosa, Rozangela Curi; de Fávere, Valfredo Tadeu

    2012-05-01

    The aim of this study was to evaluate the efficacy of the treatment of acid mine drainage (AMD) with calcinated coal mining waste using Allium cepa L. as a bioindicator. The pH values and the concentrations of aluminum, iron, manganese, zinc, copper, lead and sulfate were determined before and after the treatment of the AMD with calcinated coal mining waste. Allium cepa L. was exposed to untreated and treated AMD, as well as to mineral water as a negative control (NC). At the end of the exposure period, the inhibition of root growth was measured and the mean effective concentration (EC(50)) was determined. Oxidative stress biomarkers such as lipid peroxidation (TBARS), protein carbonyls (PC), catalase activity (CAT) and reduced glutathione levels (GSH) in the fleshy leaves of the bulb, as well as the DNA damage index (ID) in meristematic cells, were evaluated. The results indicated that the AMD treatment with calcinated coal mining waste resulted in an increase in the pH and an expressive removal of aluminum, iron, manganese and zinc. A high sub-chronic toxicity was observed when Allium cepa L. was exposed to the untreated AMD. However, after the treatment no toxicity was detected. Levels of TBARS and PC, CAT activity and the DNA damage index were significantly increased (P<0.05) in Allium cepa L. exposed to untreated AMD when compared to treated AMD and also to negative controls. No significant alteration in the GSH content was observed. In conclusion, the use of calcinated coal mining waste associated with toxicological tests on Allium cepa L. represents an alternative system for the treatment and biomonitoring of these types of environmental contaminants. Copyright © 2011 Elsevier Inc. All rights reserved.

  19. Susceptibilidad antimicrobiana de Staphylococcus aureus sensible, con sensibilidad "BORDERLINE" y resistentes a la meticilina

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    Carlos Alberto Mendoza Ticona

    2003-10-01

    Full Text Available Objetivo: Determinar las susceptibilidad a diversos antibacterianos de tres categorías de S. aureus según su sensibilidad a la meticilina. Materiales y Métodos: Se aislaron 76 cepas de S. aureus que colonizaban pacientes y personal de salud de tres servicios del Hospital Honorio Delgado de Arequipa, de los cuales 36 fueron sensibles a meticilina (MSSA, 15 con susceptibilidad "borderline" (BORSA y 25 fueron resistentes a la meticilina (MRSA. Se les sometió a antibiograma a 14 antibacterianos. Resultados: El único antibiótico al cual todas los aislamientos fueron sensibles fue vancomicina. Los MRSA fueron resistentes a todos los b-lactámicos excepto imipenem que obtuvo 64% de susceptibilidad. Los BORSA mantuvieron un 80% de sensibilidad a cefalotina y un 100% al imipenem. Los antibióticos cefalotina, imipenem, ciprofloxacina, cotrimoxazol, rifampicina, eritromicina, tetraciclina, cloranfenicol, lincomicina y gentamicina tuvieron sensibilidades variables (p<0.01 entre las tres categorías; una mayor proporción de MSSA fue sensible a estos antibióticos, luego los BORSA y finalmente los MRSA. Las cepas multi-resistentes fueron mucho más frecuentes en los MRSA que en los otros dos grupos (p <0.01. Conclusión: Existe en nuestro medio cepas de S. aureus con resistencia "borderline" a meticilina. Hay diferencias entre la sensibilidad a diversos antimicrobianos entre los tres grupos de S. aureus. Esta diferenciación podría beneficiar una terapia antimicrobiana más racional. Se debe aplicar en todo aislamiento de S. aureus las recomendaciones internacionales para la detección de meticilino resistencia.

  20. Dissection of Trichoderma longibrachiatum-induced defense in onion (Allium cepa L.) against Fusarium oxysporum f. sp. cepa by target metabolite profiling.

    Science.gov (United States)

    Abdelrahman, Mostafa; Abdel-Motaal, Fatma; El-Sayed, Magdi; Jogaiah, Sudisha; Shigyo, Masayoshi; Ito, Shin-Ichi; Tran, Lam-Son Phan

    2016-05-01

    Trichoderma spp. are versatile opportunistic plant symbionts that can cause substantial changes in the metabolism of host plants, thereby increasing plant growth and activating plant defense to various diseases. Target metabolite profiling approach was selected to demonstrate that Trichoderma longibrachiatum isolated from desert soil can confer beneficial agronomic traits to onion and induce defense mechanism against Fusarium oxysporum f. sp. cepa (FOC), through triggering a number of primary and secondary metabolite pathways. Onion seeds primed with Trichoderma T1 strain displayed early seedling emergence and enhanced growth compared with Trichoderma T2-treatment and untreated control. Therefore, T1 was selected for further investigations under greenhouse conditions, which revealed remarkable improvement in the onion bulb growth parameters and resistance against FOC. The metabolite platform of T1-primed onion (T1) and T1-primed onion challenged with FOC (T1+FOC) displayed significant accumulation of 25 abiotic and biotic stress-responsive metabolites, representing carbohydrate, phenylpropanoid and sulfur assimilation metabolic pathways. In addition, T1- and T1+FOC-treated onion plants showed discrete antioxidant capacity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) compared with control. Our findings demonstrated the contribution of T. longibrachiatum to the accumulation of key metabolites, which subsequently leads to the improvement of onion growth, as well as its resistance to oxidative stress and FOC. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  1. Utilidad del modelo animal ratón-pulmón para evaluar la virulencia de posibles cepas vacunales de Shigella spp

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    Olga M. Martínez

    2008-04-01

    Full Text Available Shigella flexneri y Shigella sonnei, como cualquier otra especie del género Shigella, se sitúan entre los principales agentes etiológicos de las enfermedades diarreicas agudas, sobre todo aquellas que ocurren en los países en vías de desarrollo, aunque por la baja dosis infectante de este enteropatógeno no se excluyen los países desarrollados. Esta situación conlleva a la elaboración de vacunas para prevenir esta enfermedad y la necesidad de un modelo animal que pruebe la eficacia protectora e inmunogénica de posibles candidatos vacunales contra la shigellosis, situación que ha motivado numerosos estudios por la dificultad de demostrar la enteropatía intestinal en los monos y humanos. Lo anteriormente expuesto, más la capacidad de Shigella spp para mostrar resistencia a los antimicrobianos, motivó la realización de este trabajo. En el mismo se constató la utilidad del modelo animal ratón-pulmón para evaluar la virulencia de candidatos vacunales a partir de este microorganismo. Se utilizó la técnica de inoculación intranasal con una concentración entre 107 y 109 UFC de cepas de Shigella flexneri y Shigella sonnei. Por todos los resultados obtenidos con el modelo animal ratón-pulmón se concluyó que este modelo puede ser eficiente para los estudios preclínicos de cualquier candidato vacunal a partir de Shigella spp.

  2. Mecanismo de selección de consorcios bacterianos compatibles con A. ferrooxidans y A. thiooxidans en procesos de biodesulfurización de carbón

    Directory of Open Access Journals (Sweden)

    Gerardo Andrés Caicedo

    2010-01-01

    Full Text Available A nivel de erlenmeyer, se evaluaron procesos de biodesulfurización de un carbón rico en azufre con un tamaño de partícula entre 0,24 cm - 0,06 cm, empleando consorcios bacterianos de cepas compatibles con Acidithiobacillus ferrooxidans y compatibles con Acidithiobacillus thiooxidans. Se analizaron los principales factores fisicoquímicos que pueden influir, con monitoreos de pH, potencial redox en el líquido lixiviante y sulfato en solución y precipitado, producido a partir de compuestos reducidos de azufre presentes en el carbón y en el medio. De acuerdo con los resultados obtenidos, se alcanzó 71,50% de sulfato producido con relación al máximo posible después de 16 días de proceso. Estos resultados son claves para la escogencia de cepa a la hora de trabajar procesos de biodesulfurización a escala mayor.

  3. DESEMPEÑO DE UNA PELÍCULA DE MAÍZ AZUL EN EL ENVASADO DE UN QUESO DE HUMEDAD INTERMEDIA

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    CECILIA ROJAS DE G.

    Full Text Available La PCT/MX2009/000092, describe un método para obtener películas a partir de harina de cereales mediante método de "casting" y empleocomo almohadillas antimicrobianas yenvases termosellables para alimentos secos. Debido a su sensibilidad al agua y baja resistencia mecánica para contener alimentos de humedad intermedia; se reformuló la matriz biopolimérica para mejorar ambos parámetros.La película derivada de la reformulación fue caracterizada mecánicamente (ASTM D1708, se midió el "swelling" y se observó mediante microscopía electrónica de barrido.El desempeño de la película fue evaluado envasando unalimento perecedero: un quesosemimadurado,sometiéndolo a almacenamiento refrigerado durante 2 meses. Control: se emplearon queso del mismo lote envasado al vacío en película alta barrera. Durante los estudios de estabilidad en anaquel se evaluaron parámetros críticos microbiológicos como fisicoquímicos para el alimento envasado.Se observó que si bien las películas activas controlaron la carga microbiana, la integridad del envase se vio alterada debido a absorción de agua por contacto con el alimento, observándose ruptura de la estructura e integridad del biopolímeroy en consecuencia, exposición del producto y término de su vida útil.

  4. LSSP-PCR para la identificación de polimorfismos en el gen cry1B en cepas nativas de Bacillus thuringiensis

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    Martha Ilce Orozco Mera

    2012-01-01

    Full Text Available Título en ingles: LSSP-PCR to identify polymorphisms in the gene cry1B of Bacillus thuringiensis native strain Resumen: Se estandarizó la técnica LSSP-PCR (reacción en cadena de la polimerasa con un único oligonucleótido en condiciones de baja astringencia, para identificar polimorfismos del gen cry1B en aislamientos nativos de Bacillus thuringiensis (Bt. Se evaluaron 164 aislamientos nativos colombianos identificándose el gen cry1Ba en 11 de estos aislamientos. Los 11 fragmentos amplificados, junto con el de la cepa de referencia Bt subsp. aizawai HD137, se analizaron por LSSP-PCR y los patrones electroforéticos obtenidos se compararon cualitativamente. Con los productos amplificados mediante el oligonucleótido directo se construyó un dendrograma utilizando UPGMA que  mostró tres agrupamientos con similitud de 83, 79 y 68%. La agrupación con 68% de similaridad correspondió al aislamiento nativo BtGC120 que presentó el patrón de bandas más variable. Con el oligonucleótido reverso el aislamiento BtGC120 mostró una menor variabilidad (43%. La secuencia nucleotidica obtenida de este fragmento de 806 pares de bases mostró una identidad de 93% con la secuencia de los genes cry1Bc1 de Bt morrisoni y cry1Bb1 de la cepa BT-EG5847. Se predijo del marco de lectura +3 una proteína de 268 residuos aminoácidicos, con 88% de identidad con la proteína Cry1Bc. Esta  secuencia reveló dos dominios, una endotoxina N implicada en la formación del poro y otra endotoxina M relacionada en el reconocimiento del receptor. La evaluación biológica del aislamiento BtGC120 sobre larvas de primer instar del insecto plaga Spodoptera frugiperda, mostró una CL50 de 1,896 ng de proteína total por cm2. Este estudio muestra que la LSSP-PCR es una técnica que permite identificar de una manera específica variaciones en las secuencias de los genes cry de Bt, con potencialidad de encontrar nuevos genes con novedosas actividades biológicas. Abstract

  5. Inhibición in vitro de aislamientos nativos de Trichoderma en presencia de la cepa comercial T22

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    Gabriela Hernández – Ramírez

    2013-01-01

    Full Text Available Título en ingles: In vitro inhibition from native isolates of Trichoderma against commercial strain T22.  Título corto: Inhibición in vitro de aislamientos nativos Resumen El objetivo de este trabajo fue evaluar 14 aislamientos nativos del género Trichoderma (Tn obtenidos de la rizósfera del cultivo de caña de azúcar de acuerdo con su efecto de inhibición In vitro en cultivos duales contra la cepa comercial de Trichoderma harzianum (T22. Los aislamientos nativos con mayor índice de inhibición fueron los denominados Palma (T12 y Hubero (T11, con un valor de 2, mientras que T01, T04, T05 y T08 presentaron un valor de 1. Además, el mayor porcentaje de inhibición fue causado por el aislamiento T03, con una media de 92.32 ± 2.64%, sin diferencias estadísticas con los nativos T07, con un valor inhibición de 88.99 ± 1.46%, T01 87.93 ± 2.28%, y T12; 87.71 ± 3.16. En este caso, los aislamientos T07 y T12 mostraron capacidad inhibitoria con mayores atributos para su selección como agentes de control biológico. Al evaluar la tolerancia In vitro de los aislamientos nativos de Trichoderma a la actividad del agua (aw, se observó efecto significativo sobre el desarrollo micelial de los aislamientos de Trichoderma nativo evaluados. Los aislamientos T03 (7919.3 ± 932.53 y T12 (6388.2 ± 623.40 presentaron el mejor desarrollo radial. Estos resultados sugieren que los aislamientos T03 y T12 podrían desarrollarse mejor bajo condiciones de estrés hídrico. Por lo anterior, concluimos que los aislamientos T07 y T12, son los mejores candidatos como agentes inhibidores y se sugiere evaluarlos In vitro o invernadero contra el agente causal de la enfermedad del muermo rojo en caña de azúcar (Colletotrichum falcatum. Palabras clave: Control Biológico; tolerancia;  ompetencia; estrés hídrico; actividad de agua. Abstract The objective of this work was to evaluate 14 native e Trichoderma genus isolates (Tn obtained from the rhyzosphere of

  6. Effect of gamma irradiation on the storage and vitamin C concentration of allium cepa onion samples

    International Nuclear Information System (INIS)

    Saleh, M. A. M.

    2013-07-01

    This study was done to investigate the effect of gamma irradiation on storage, germination and vitamin C concentration of local onion (Allium cepa). 5 onion samples were irradiated with 5 different radiation doses (0.1, 0.2, 0.3, 0.4, and 0.5 k Gray) using cobalt-60 irradiator (Nor din) compared to non irradiated samples as controls. The irradiated and control onions were stored at room temperature for three months. The results of this study showed that the non irradiated samples were either deteriorated or grown while all the irradiated samples were not. Regarding the concentration of vitamin C it was clear that it decreased with the dose increase from 30.53 to 14.44 mg/100 g. This study concluded that the irradiation is very effective in prevention of spoilage, elongation of germination period and decrease of vitamin C concentration.(Author)

  7. Effects of bromine on mitosis in root-tips of Allium cepa

    Energy Technology Data Exchange (ETDEWEB)

    Chury, J; Slouka, V

    1949-01-01

    The root-tips of Allium cepa, 1.5-2 cm. long, were exposed to pure bromine vapor for five minutes. The root-tips were then washed for ten minutes in water, and kept in fresh-water at a temperature of 20-24/sup 0/C. Squash preparations were made and stained according to the method of Darlington and La Cour. Bromine acting for five minutes on the root-tips of Allium has a specific effect on the cell nucleus in the resting stage. The effects induced are shown thirty-six hours after treatment by spindle abnormalities in metaphase and anaphase, and result in polyploidy in a large number of cells. Bromine produces chromosome and chromatid fragmentation; the latter may be followed by reunion. The effect of the bromine is cumulative and depends on the time which elapses between treatment and fixation. The cytological effects induced by bromine strongly suggest that it is another specific mutafacient chemical.

  8. Caracterização de cepas de P. falciparum coletadas de pacientes recrudescentes

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    Virgilio E. do Rosário

    1985-10-01

    Full Text Available A caracterização de cepas de P. falciparum coletadas de três pacientes internados no Centro de Provas Clínico-Farmacológicas do Hospital Barros Barreto (Belém, PA, através da eletroforese em acetato de celulose para duas enzimas (GPI e ADA, assim como através de testes de sensibilidade para duas drogas, cloroquina e mefloquina, permitiram observações da variação dos tipos enzimáticos em um dos pacientes e modificação no nível de sensibilidade à cloroquina em outro, tanto em amostras obtidas da infecção original, como em cada uma das recrudescências.

  9. Changes in endogenous growth inhibitors in onion bulbs (Allium cepa L. cv. Sochaczewska during storage

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    Elżbieta Kielak

    2013-12-01

    Full Text Available Changes in inhibitor activity in the onion bulbs (Allium cepa L. cv. Sochaczewska during storage were investigated. Onions were dried under an umbrella roof until October 15th or November 15th and thereafter stored in a cold-room at 0-1°C until May 15th. The activity of inhibitors fluctuated markedly during the storage period. At least two peaks and two decreases of inhibitor activity were observed. The weather conditions seemed to strongly influence the level and the date of appearance of inhibitors in onions. Higher inhibitor activity is usually connected with better storage and less sprouting of onions during storage. Prolonged drying under an umbrella roof enhanced onion quality after storage only in these cases when it actually improved the drying of onions.

  10. Sulphur management in onion (Allium cepa) cultivation in hills of Himachal Pradesh.

    Science.gov (United States)

    Jaggi, R C; Sharma, R K

    2010-05-01

    Field experiment were conducted at CSK HPKV Research Farm, Palampur during Rabi seasons of 2000-01 and 2001-02, to study the response of onion (Allium cepa var Patna red) at four sulphur levels (0, 15, 30 and 60 kg ha(-1)) applied through Gypsum and S95. The analysis was done to allocate the limited availability of sulphur for maximizing net profit over fertilizer cost. The results show that the dose of sulphur under its full availability is 43.02 kg ha(-1). But under its scarce availability the maximum benefit would occur when it is applied up to 32.11 kg ha(-1) followed by even distribution of fertilizer i.e. 20 kg ha(-1). The returns following sulphur application at these rates, would be Rs 69340, 73092 and 68700 ha(-1) respectively.

  11. Frecuencia de los genotipos babA2, oipA y cagE de Helicobacter pylori en pacientes colombianos con enfermedades gastroduodenales.

    Directory of Open Access Journals (Sweden)

    Andrés Javier Quiroga

    2005-09-01

    Full Text Available Introducción. La infección con Helicobacter pylori está asociada con el desarrollo de diferentes enfermedades gastroduodenales. Varios genes de virulencia de H. pylori se han relacionado con mayor riesgo de enfermedad gástrica. Objetivos. El propósito de este trabajo fue determinar las posibles asociaciones entre la presencia de los genes vacA, cagA, cagE, babA2 y oipA en aislamientos de H. pylori de pacientes colombianos y las diferentes consecuencias clínicas de la infección. Materiales y métodos. Mediante PCR se evaluaron los genotipos cagA, vacA, cagE, oipA y babA2 en 166 aislamientos de H. pylori provenientes de 50 pacientes con úlcera péptica, 39 con gastritis crónica no atrófica, 26 con gastritis crónica atrófica, 26 con metaplasia intestinal y 25 con adenocarcinoma gástrico. Resultados. La frecuencia de los genotipos cagA, cagE, babA2 y oipA fue de 73%, 75%, 48% y 74%, respectivamente. El 64% (100/157 de los aislamientos presentó el genotipo citotóxico vacAs1m1/cagA positivo/cagE positivo. Se observó una mayor frecuencia de cepas citotóxicas en pacientes con cáncer (84%, metaplasia (91% y úlcera (81% en comparación con pacientes con gastritis no atrófica (50% (p=0,002, 0,008 y 0,007, respectivamente. La frecuencia de oipA y babA2 fue mayor en cepas citotóxicas que en cepas no citotóxicas (oipA: 81% vs. 52%, p=0,003; babA2: 58% vs. 12%, p=0,000. No se observaron diferencias significativas en la frecuencia de los genes oipA o babA2 solos o en asociación con vacA y cagA/cagE y las diferentes enfermedades gastroduodenales. Conclusiones. No se encontraron evidencias que sugieran que los genes babA2 u oipA puedan servir como marcadores de ulcerogénesis o carcinogénesis en esta población, solos o en asociación con cagA, cagE o vacA.

  12. Cytotoxicity of Cheese and Cheddar Cheese food flavorings on Allim cepa L root meristems

    Directory of Open Access Journals (Sweden)

    A. G. Moura

    Full Text Available Abstract Despite their great importance for the food industry, flavorings, in general, raise a number of questions regarding their cytotoxicity, mutagenicity and carcinogenicity, since, in the literature, there are few studies found evaluating the toxicity on the systemic and cellular level, of these chemical compounds. The root meristems of Allium cepa (onion are widely used for the assessment of toxicity of chemical compounds of interest. Thus, this study aimed to evaluate, in A. cepa meristematic cells, individually and in combination at the cellular level, the toxicity of synthetic Cheese and Cheddar Cheese food flavorings, identical to the natural, at doses of 1.0 and 2.0 mL, at exposure times of 24 and 48 hours. In combination we used 0.5 mL of Cheese flavor associated with 0.5 mL of Cheddar flavor; and 1.0 mL of Cheese flavor associated with 1.0 mL of Cheddar flavor, at exposure times of 24 and 48 hours. For these evaluations, we used groups of five onion bulbs, which were first embedded in distilled water and then transferred to their respective doses. The root tips were collected and fixed in acetic acid (3:1 for 24 hours. The slides were prepared by crushing and were stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 for each control and exposure time. The mitotic indices calculated and cellular aberrations observed were subjected to statistical analysis using the chi-square test (p <0.05. No chromosomal abnormalities nor those of mitotic spindle were observed for the treatments performed. The results, both individually and in combination, showed that the flavorings under study significantly reduced the cell division rate of the test system cells used. Therefore, under the conditions studied, the two flavorings were cytotoxic.

  13. Assessment of genotoxic effects of pesticide and vermicompost treated soil with Allium cepa test

    Directory of Open Access Journals (Sweden)

    Shivika Datta

    2018-07-01

    Full Text Available Soil forms a huge reservoir of nutrients that sustains life on earth. Anthropogenic and natural impacts have led to degradation of land which declines the overall quality of soil, water or vegetation. The present study involves comparison of genotoxicity of soil procured from two different agricultural sites, pesticide treated soil (PTS and vermicompost treated soil (VTS. The soil was physico-chemically characterized and showed significant differences in terms of cytotoxicity (root length; mitotic index and genotoxicity (chromosomal aberrations in Allium cepa test. The mitotic index of the control after 24 and 48 h was found to be 26.1 ± 1.6 and 26.1 ± 1.3 respectively. Mitotic index was reduced to 10.3 ± 0.9 and 9.7 ± 0.6 in 100% PTS and 24.4 ± 1.7 and 25.4 ± 0.8 in 100% VTS after 24 and 48 h of exposure, respectively. Clastogenic aberrations were found to be highest (54.5% in 100% PTS which was significantly different from VTS extract. The PTS extracts incurred significantly more cytotoxic and genotoxic effects on A. cepa in comparison to VTS. The result indicates that addition of vermicompost in agriculture field acts as soil ameliorator and plays an important role in promotion of cell division and proliferation, hence good for the plant health and crop productivity.

  14. Molecular and Functional Characterization of FLOWERING LOCUS T Homologs in Allium cepa

    Directory of Open Access Journals (Sweden)

    Ranjith Kumar Manoharan

    2016-02-01

    Full Text Available Onion bulbing is an important agricultural trait affecting economic value and is regulated by flowering-related genes. FLOWERING LOCUS T (FT-like gene function is crucial for the initiation of flowering in various plant species and also in asexual reproduction in tuber plants. By employing various computational analysis using RNA-Seq data, we identified eight FT-like genes (AcFT encoding PEBP (phosphatidylethanolamine-binding protein domains in Allium cepa. Sequence and phylogenetic analyses of FT-like proteins revealed six proteins that were identical to previously reported AcFT1-6 proteins, as well as one (AcFT7 with a highly conserved region shared with AcFT6 and another (comp106231 with low similarity to MFT protein, but containing a PEBP domain. Homology modelling of AcFT7 proteins showed similar structures and conservation of amino acids crucial for function in AtFT (Arabidopsis and Hd3a (rice, with variation in the C-terminal region. Further, we analyzed AcFT expression patterns in different transitional stages, as well as under SD (short-day, LD (long-day, and drought treatment in two contrasting genotypic lines EM (early maturation, 36101 and LM (late maturation, 36122. The FT transcript levels were greatly affected by various environmental factors such as photoperiod, temperature and drought. Our results suggest that AcFT7 is a member of the FT-like genes in Allium cepa and may be involved in regulation of onion bulbing, similar to other FT genes. In addition, AcFT4 and AcFT7 could be involved in establishing the difference in timing of bulb maturity between the two contrasting onion lines.

  15. Sistema constructivo de tecnología intermedia como solución al problema de vivienda en las áreas marginales y socioeconómicamente deprimidas de una ciudad

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    Alva Mejía, Alberto

    1984-07-01

    Full Text Available The constructive system of intermediate technology offers to the population the possibility to construct his dwelling by the way of autoconstruction in stages. Its aim is to project and construct at least 10 % of the demands for housing in Lima, within a three years limit, and to support the creation of Communal Crafts Workshops. The system is to join several prefabricated elements as girders, sheets and concrete arches with vegetal fibres, wooden roof frames, etc.El sistema constructivo de tecnología intermedia ofrece a la población la posibilidad de construir su vivienda bajo la modalidad de autoconstrucción por etapas. Su meta es proyectar y construir, al menos, el 10 % de las viviendas demandadas en la actualidad en Lima con un plazo de 3 años, y apoyar la formación de talleres artesanales comunales. El sistema consiste en la unión de varios elementos prefabricados como son viguetas, láminas y arcos de hormigón con fibras vegetales, tijerales de madera, etcétera.

  16. BIOLOGICALLY ACTIVE SUBSTANCES OF LAVANDULA X INTERMEDIA EMERIC EX LOISEL (LAMIACEAE

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    A. E. Paliy

    2016-01-01

    Full Text Available Data about qualitative and quantitative composition of biologically active substances (volatile and phenolic compounds in water- ethanol extract of Lavandula x intermedia Emeric ex Loisel (Lamiaceae cv. ‘Bora’ bred in Nikitsky Botanical Garden are presented in the article. Concentration of volatile compounds in Lavandin extract was 398 mg/dm3 and 51 components were identified. Main volatiles in Lavandin cv. «Bora» extract were linalyl acetate (36,9% and linalool (33,5%. Content of phenolic compounds in water- ethanol extract of Lavandin was 945 mg/dm3 and 14 components were found out. Among the variety of Lavandin cv. ‘Bora’ phenolic compounds luteolin-7-O-glycoside and п-coumaric acid are predominated. The conclusion about possible use of Lavandin cv. ‘Bora’ for creation of food, cosmetic, therapeutic and preventive products is made.

  17. POLÍTICAS URBANAS PARA EL DESARROLLO Y LA INNOVACIÓN EN LAS CIUDADES INTERMEDIAS

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    Henar Pascual Ruiz-Valdepeñas

    2008-01-01

    Full Text Available En los últimos años se asiste al fortalecimiento del papel de las ciudades intermedias al estimarsesu capacidad para lograr una mayor cohesión económica, social y territorial en Europa. Las políticas territoriales se orientan a reforzar sus ventajas competitivas y a potenciar la configuración y consolidación de redes de cooperación en las que las ciudades actúan como partes de un modelo policéntrico. La valoración de los impactos de los programas europeos en las ciudades y el análisis de las políticas y estrategias urbanas, ponen de relieve la naturaleza específica de los diversos niveles de intervención pública en el territorio y la multiplicidad de las interacciones que se generan entre ellos. En el nivel local de intervención, la planificación estratégica amplía el abanico de objetivos y actuaciones y aporta nuevos instrumentos que ponen el acento en el reforzamiento de los factores de competitividad urbana.

  18. Identification of Stria Medullaris Fibers in the Massa Intermedia Using Diffusion Tensor Imaging.

    Science.gov (United States)

    Kochanski, Ryan B; Dawe, Robert; Kocak, Mehmet; Sani, Sepehr

    2018-04-01

    The massa intermedia (MI) or interthalamic adhesion is an inconsistent band spanning between bilateral medial thalami that is absent in up to 20%-30% of individuals. Little is known of its significance, especially in regard to functional pathways. Probabilistic diffusion tensor imaging (DTI) has recently been used to seed the lateral habenula and define its afferent white matter pathway, the stria medullaris thalami (SM). We sought to determine whether the MI serves as a conduit for crossing of limbic fibers such as the SM. Probabilistic DTI was performed on 10 subjects who had presence of a MI as visualized on magnetic resonance imaging. Tractography was also performed on 2 subjects without MI. Manual identification of the lateral habenula on axial T1-weighted magnetic resonance imaging was used for the initial seed region for tractography. In all subjects, the SM was reliably visualized. In 7 of the 10 subjects with MI, there was evidence of SM fibers that crossed to the ipsilateral hemisphere. Three subjects with small diameter MI did not have tractographic evidence of crossing SM fibers. Of the 7 subjects with crossing SM fibers within the MI, 5 showed predilection toward the right orbitofrontal cortex from both the left and right seed regions. Probabilistic DTI provides evidence of SM fibers within the MI. Given its anatomic location as a bridging pathway between thalami, further studies are necessary to assess its role within the limbic functional network. Copyright © 2018 Elsevier Inc. All rights reserved.

  19. Ultraviolet radiation response of two heterotropy Antarctic marine bacterial; Respuesta a la radiacion ultravioleta de dos cepas bacterianas marinas heterotrofas antarticas

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez, Edgardo A [Buenos Aires Univ. (Argentina). Facultad de Farmacia y Bioquimica; Ferreyra, Gustavo A; Mac Cormack, Walter P [Direccion Nacional del Antartico, Buenos Aires (Argentina). Instituto Antartico Argentino

    2004-07-01

    Two Antarctic marine bacterial strains, were exposed to different irradiance of ultraviolet (UV) solar radiation using several experimental protocols and interferential filters. Results showed that both, UV-A and UV-B radiation produce deleterious effects on two tested bacterial strains. The mortality values under UVB treatments were higher than those observed under UVA treatments. UVvi strain proved to be more resistant to UV radiation than the UVps strain. (author) [Spanish] Dos cepas marinas antarticas fueron expuestas a diferentes irradiancias de radiacion solar ultravioleta (UV) utilizando diferentes protocolos experimentales y filtros interferenciales. Los resultados mostraron que tanto la radiacion UV-A como UV-B produce efectos deletereos sobre las dos cepas bacterianas analizadas. Los valores de mortalidad bajo tratamiento UV-B fueron mayores que los observados bajo tratamiento UV-A. La cepa UVvi mostro mayor resistencia a la radiacion UV que la cepa UVps. (autor)

  20. First assessment of low- to medium-temperature geothermal reserves in 20 Mexican states; Primera estimacion de las reservas geotermicas de temperatura intermedia a baja en veinte estados de Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Iglesias, Eduardo R.; Torres, Rodolfo J. [Instituto de Investigaciones Electricas, Gerencia de Geotermia, Cuernavaca, Morelos (Mexico)]. E-mail: iglesias@iie.org.mx

    2009-07-15

    A first, partial, assessment is included of the low- to medium-temperature geothermal reserves in 20 Mexican States and their aggregate value. The assessment covers about 29.16% of the identified geothermal-surface manifestations in the public database. For reserve assessments, we use the volumetric method, supplemented with Montecarlo simulations and statistics, to quantify inherent uncertainties. Our estimations are presented on a state-by-state basis. We estimate the aggregated reserves of the 20 states as between 7.7 x 1016 and 8.6 x 1016 kJ, with 90% confidence. The most likely reservoir temperatures range between 60-180 degrees Celsius, with a mean of 111 degrees Celsius. Such massive amounts of recoverable energy-and the associated temperatures-are potentially important for the economic development of nearby localities and the nation. [Spanish] En este trabajo se hace una primera estimacion, parcial, de las reservas geotermicas de temperatura intermedia a baja de Mexico. La estimacion incluye 29.16% de las manifestaciones geotermicas identificadas en la base de datos publica utilizada. Para estimar las reservas se utilizo el metodo de volumen, suplementado con simulaciones por el metodo de Montecarlo, con el fin de cuantificar las incertidumbres inherentes. Las estimaciones se presentan estado por estado. Estos resultados indican que las reservas agregadas de los 20 estados considerados estan entre 7.7 x 1016 y 8.6 x 1016 kJ, con 90% de confianza. La distribucion de las temperaturas de yacimiento mas probables varia entre aproximadamente 60 y 180 grados centigrados, con un valor medio de 111 grados centigrados. La enorme magnitud de estas reservas, y sus temperaturas asociadas, son potencialmente importantes para el desarrollo economico de las poblaciones ubicadas en su cercania.

  1. Consorcios microbianos con actividad ácido-láctica promisoria aislados desde inoculantes bacterianos nativos para ensilajes

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    Byron Leoncio Díaz-Monroy

    2015-04-01

    Full Text Available El objetivo de este trabajo fue aislar y caracterizar cepas de bacterias ácido lácticas (BAL a partir de preparados microbianos (PM nativos elaborados a base de residuos agroindustriales, como suero de leche, estiércol bovino y contenido ruminal, y diseñados como inoculantes biológicos para procesos de ensilaje de residuales orgánicos poscosecha. Se evaluaron 5 tratamientos (preparados microbianos, PM + 33% de residuo inoculante, con 3 réplicas: 1 Tratamiento control sin inoculante (PMSI, 2 Suero de leche (PMSL, 3 Estiércol bovino (PMEB, 4 Combinación de suero+estiércol (PMM y 5 Contenido ruminal (PMCR; además, en todos se adicionó: melaza (20%, urea (1%, sal mineral (1% y agua (45%. En recipientes plásticos se fermentaron tapados durante 96 horas, a 14 °C; de allí se obtuvieron 3 muestras de 500 mL de cada PM, se sembraron en agar MRS (Man, Rogosa y Sharpe y se incubaron a 37 ºC durante 72 h bajo condiciones anaerobias (AnaeroGen, Oxoid. Las cepas de BAL son catalasa y oxidasa negativas, cocos, coco-bacilos y bacilos Gram positivos no esporulados. La identificación bioquímica se realizó con el sistema API-50CHL (BioMérieux, France y la base de datos APILAB Plus versión 3.3.3. Se aislaron 39 cepas de BAL, 4 del PMSI (2 Lactobacillus plantarum, 1 L. brevis y 1 Lactococcus lactis, 12 del PMSL (3 Lactobacillus rhamnosus, 3 L. casei, 2 L. acidophilus, 2 L. paracasei y 2 L. fermentum, 7 del PMEB (3 Streptococcus bovis, 2 Lactobacillus plantarum y 2 Leuconostoc mesenteroides, 10 del PMM (3 Lactobacillus casei, 2 L. plantarum, 2 L. acidophilus, 1 L. fermentum, 1 L. rhamnosus y 1 L. paracasei y 6 del PMCR (2 Lactobacillus vitulinus, 2 L. ruminus, 1 L. reuteri y 1 Pediococcus damnosus. En conclusión, la mejor fuente de cepas de BAL fue PMSL, que le otorga ventajas como posible inoculante bacteriano para ensilajes. En todos los PM se identificaron cepas homofermentativas y heterofermentativas, asociadas en consorcios

  2. Evaluación de la reacción en cadena de la polimerasa para el diagnóstico de la brucelosis en un rebaño lechero infectado con Brucellaspp Assessment of polymerase chain reaction (PCR to diagnose brucellosis in a Brucella infected herd

    Directory of Open Access Journals (Sweden)

    O. Lavaroni

    2004-09-01

    Full Text Available Para el diagnóstico de la brucelosis bovina en muestras de sangre y/o leche, se comparó la reacción en cadena de la polimerasa (PCR con el aislamiento in vitro de Brucella abortus, las pruebas serológicas defijación del complemento (FC e inmunoenzimáticas de competición (ELISA-C en suero e indirecto (ELISA-I en leche. Se analizaron muestras de vacas lecheras de un rebaño infectado “A”, vacunadas con B. abortus cepa 19 antes de los 8 meses de edad y revacunadas con B. abortus cepa RB51 como adultas (n= 99 y de otro “B”, libre de brucelosis (n=100, como control. En A, la PCR identificó 14 vacas infectadas con B. abortus: nueve con cepa silvestre y cinco con cepa silvestre y RB51. No se identificó B. abortus cepa 19. El biotipo 1 se aisló en un caso. Las 14 vacas infectadas con la cepa silvestre resultaron positivas en las tres pruebas serológicas. En B, por PCR no se identificó Brucella. Las pruebas serológicas mostraron una sensibilidad del 100% respecto de PCR. La especificidad para FC, ELISA-C y ELISA-I fue del 100%, 99% y 95%, respectivamente. Se concluye que la PCR sería útil como complemento de las pruebas serológicas o cuando no hay un resultado concluyente.The diagnosis of bovine brucellosis using PCR in blood and milk samples from two dairy herds were compared to in vitro isolation, complement fixation test (CF, competitive ELISA (C-ELISA in serum, and indirect ELISA (I-ELISA in milk. Samples were obtained from 99 cows vaccinated with Brucella abortus strain 19, from a naturally infected herd (A, whose cows were also vaccinated with B. abortus strain RB51 as adults, and 100 from brucellosis free herd (B. In herd A, PCR identified 14 B. abortus infected cows: nine infected with wild type, and five with wild type and RB51, B. abortus S 19 was not identified. B. abortus biotype 1 was isolated from one cow. All cows infected with a wild strain of B. abortus were positive in serologic tests. Brucella was not found in

  3. Amelioration of atopic-like skin conditions in NC/Tnd mice by topical application with distilled Alpinia intermedia Gagnep extracts.

    Science.gov (United States)

    Amagai, Yosuke; Katsuta, Chihiro; Nomura, Yoshihiro; Oida, Kumiko; Matsuda, Kenshiro; Jang, Hyosun; Ahn, Ginnae; Hamasaki, Tetsuyoshi; Matsuda, Hiroshi; Tanaka, Akane

    2017-11-01

    Alpinia intermedia, a perennial plant that belongs to the Zingiberaceae family, has been used in folk medicine for a long time in the southern region of Japan. Because skin care is an effective approach that enables patients to manage their atopic dermatitis (AD), various herbal ingredients with few adverse effects have been evaluated for use in AD patients in recent years. In this study, we examined whether distilled extracts obtained from A. intermedia were beneficial for AD-like skin conditions in NC/Tnd mice. Topical application with the A. intermedia extracts significantly reduced the severity of AD, transepidermal water loss and scratching behavior in the mice. Supplementation of the extracts to cell cultures suppressed the expression of Tslp mRNA in PAM212 keratinocytes, degranulation in bone marrow-derived cultured mast cells (BMCMC), and neurite outgrowth in PC12 cells and dorsal root ganglia. In addition, the component analysis revealed that β-pinene was a major constituent of the A. intermedia extracts. The inhibitory effects of β-pinene both in vivo and in vitro were also demonstrated. These results indicate that topical application with the A. intermedia extract to the skin of NC/Tnd mice improved the condition of the skin by suppressing multiple inflammatory responses. The extracts may become novel skin-care remedies for AD patients. © 2017 The Authors. The Journal of Dermatology published by John Wiley & Sons Australia, Ltd on behalf of Japanese Dermatological Association.

  4. Acquisition of complement inhibitor serine protease factor I and its cofactors C4b-binding protein and factor H by Prevotella intermedia.

    Science.gov (United States)

    Malm, Sven; Jusko, Monika; Eick, Sigrun; Potempa, Jan; Riesbeck, Kristian; Blom, Anna M

    2012-01-01

    Infection with the Gram-negative pathogen Prevotella intermedia gives rise to periodontitis and a growing number of studies implies an association of P. intermedia with rheumatoid arthritis. The serine protease Factor I (FI) is the central inhibitor of complement degrading complement components C3b and C4b in the presence of cofactors such as C4b-binding protein (C4BP) and Factor H (FH). Yet, the significance of complement inhibitor acquisition in P. intermedia infection and FI binding by Gram-negative pathogens has not been addressed. Here we show that P. intermedia isolates bound purified FI as well as FI directly from heat-inactivated human serum. FI bound to bacteria retained its serine protease activity as shown in degradation experiments with (125)I-labeled C4b. Since FI requires cofactors for its activity we also investigated the binding of purified cofactors C4BP and FH and found acquisition of both proteins, which retained their activity in FI mediated degradation of C3b and C4b. We propose that FI binding by P. intermedia represents a new mechanism contributing to complement evasion by a Gram-negative bacterial pathogen associated with chronic diseases.

  5. Primer aislamiento de Encephalitozoon intestinalis a partir de muestra de materia fecal de un paciente colombiano con sida

    Directory of Open Access Journals (Sweden)

    Ana Luz Galván

    2008-09-01

    Full Text Available Introducción. Los microsporidios son agentes de infecciones oportunistas en pacientes con sida y con trasplantes, principalmente. Enterocytozoon bieneusi y Encephalitozoon intestinalis son los más frecuentes, asociados con infecciones entéricas. Los cultivos celulares han contribuido al conocimiento de los microsporidios. En Colombia no se han obtenido aislamientos provenientes de pacientes con microsporidiosis y, por consiguiente, no existen cepas autóctonas de los mismos. Objetivo. Establecer el cultivo celular de microsporidios intestinales a partir de materia fecal de pacientes parasitados. Materiales y métodos. Se realizó concentración agua-éter de la materia fecal positiva para microsporidios y el sedimento resultante se trató con una mezcla de antibióticos y antimicóticos durante 18 horas a 37 oC. Se inocularon células Vero previamente cultivadas en placas de 24 pozos y en medio RPMI con suplemento de suero bovino fetal al 10% y antibióticos, con las esporas concentradas. Los cultivos se mantuvieron a 37 oC al 5% de CO2. Se cambió de medio cada dos días y se evaluó la presencia de esporas en los sobrenadantes mediante Gram-cromótropo rápido en caliente. Resultados. En la segunda semana después de la infección, se encontraron esporas de microsporidios con morfología y coloración características. Mediante PCR se determinó que el microsporidio encontrado correspondía a la especie E. intestinalis. Conclusión. Se estableció el cultivo in vitro de microsporidios de materia fecal. Este protocolo es importante para la obtención y el mantenimiento de cepas autóctonas en Colombia, y contribuirá a las investigaciones de aspectos bioquímicos, inmunológicos y epidemiológicos de dichas cepas.

  6. Molecular characterization of severe and mild cases of influenza A (H1N1 2009 strain from Argentina Caracterización molecular de cepas de influenza A (H1N1 2009 de casos leves y graves de la Argentina

    Directory of Open Access Journals (Sweden)

    Elsa Baumeister

    2010-12-01

    Full Text Available While worldwide pandemic influenza A(H1N1 pdm case fatality rate (CFR was 0.4%, Argentina's was 4.5%. A total of 34 strains from mild and severe cases were analyzed. A full genome sequencing was carried out on 26 of these, and a partial sequencing on the remaining eight. We observed no evidence that the high CFR can be attributed to direct virus changes. No evidence of re-assortment, mutations associated with resistance to antiviral drugs, or genetic drift that might contribute to virulence was observed. Although the mutation D225G associated with severity in the latest reports from the Ukraine and Norway is not observed among the Argentine strains, an amino acid change in the area (S206T surrounding the HA receptor binding domain was observed, the same previously established worldwide.Mientras que la tasa de letalidad (CFR para (H1N1pdm en todo el mundo era del 0.4%, en la Argentina la mortalidad observada fue de 4.5%. La secuenciación del genoma completo de 26 cepas de virus argentinos de influenza A (H1N1pdm de casos leves y graves y de 8 cepas secuenciadas parcialmente no mostró evidencia de que la elevada tasa de letalidad se pueda atribuir directamente a cambios en el virus. No se encontraron hallazgos de recombinación, de mutaciones asociadas con la resistencia a los medicamentos antivirales ni de variaciones genéticas que puedan contribuir a la virulencia observada. Si bien la mutación D225G asociada con la gravedad, comunicada en informes procedentes de Ucrania y Noruega, no se ha encontrado en las cepas argentinas estudiadas, se ha observado un cambio aminoacídico en la región (S206T en torno al dominio del sitio de unión al receptor en la HA, el mismo hallado en cepas distribuidas alrededor del mundo.

  7. Diversidad genética de la región variable de los genes msp1a y msp4 en cepas de Anaplasma marginale de México

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    Rafael Jiménez Ocampo

    2012-01-01

    Full Text Available La anaplasmosis es de difícil control debido a la diversidad genética de la rickettsia. La proteína Msp1a, compuesta de repetidos variables de entre 23 y 31 aminoácidos en su región variable y la proteína Msp4, son dos de las proteínas de superficie más estudiadas en A. marginale y han sido ampliamente usadas como marcadores genéticos en la caracterización de cepas de A. marginale de diferentes orígenes geográficos. En este trabajo se analizaron, la región variable de la proteína Msp1a y la proteína Msp4 de 10 cepas mexicanas. En el caso de Msp1a, se observó un patrón de segregación que contiene los repetidos (alpha, beta, beta, gama en diferentes modalidades a lo largo de aislamientos del Golfo de México, principalmente en zonas de estabilidad enzoótica, mientras que la máxima variabilidad se presentó en Tamaulipas, en aislamientos de un brote de la enfermedad, es decir en zonas de inestabilidad. La diversidad observada no es tan extensa como se esperaba y, misma que se puede explicar por la presión que el sistema inmune del hospedero ejerce contra la rickettsia y los mecanismos de esta última para evadirla. En el caso de la proteína Msp4, la secuencia fue altamente conservada tanto en nucleótidos como en aminoácidos para los aislados en estudio, aunque, se observan diferencias con lo previamente reportado en México para este marcador.

  8. Hierarchical porous carbon derived from Allium cepa for supercapacitors through direct carbonization method with the assist of calcium acetate

    KAUST Repository

    Xu, Jinhui; Zhang, Wenli; Hou, Dianxun; Huang, Weimin; Lin, Haibo

    2017-01-01

    In this paper, a direction carbonization method was used to prepare porous carbon from Allium cepa for supercapacitor applications. In this method, calcium acetate was used to assist carbonization process. Scanning electron microscope (SEM) and N2 adsorption/desorption method were used to characterize the morphology, Brunauer-Emmett-Teller (BET) specific surface area and pore size distribution of porous carbon derived from Allium cepa (onion derived porous carbon, OPC). OPC is of hierarchical porous structure with high specific surface area and relatively high specific capacitance. OPC possesses relatively high specific surface area of 533.5 m2/g. What’s more, OPC possesses a specific capacitance of 133.5 F/g at scan rate of 5 mV/s.

  9. Hierarchical porous carbon derived from Allium cepa for supercapacitors through direct carbonization method with the assist of calcium acetate

    KAUST Repository

    Xu, Jinhui

    2017-11-02

    In this paper, a direction carbonization method was used to prepare porous carbon from Allium cepa for supercapacitor applications. In this method, calcium acetate was used to assist carbonization process. Scanning electron microscope (SEM) and N2 adsorption/desorption method were used to characterize the morphology, Brunauer-Emmett-Teller (BET) specific surface area and pore size distribution of porous carbon derived from Allium cepa (onion derived porous carbon, OPC). OPC is of hierarchical porous structure with high specific surface area and relatively high specific capacitance. OPC possesses relatively high specific surface area of 533.5 m2/g. What’s more, OPC possesses a specific capacitance of 133.5 F/g at scan rate of 5 mV/s.

  10. Seleção e avaliação de cepas bacterianas para ensilagem de milho

    OpenAIRE

    Santos, Andréia de Oliveira dos

    2014-01-01

    O presente trabalho foi realizado com o objetivo de selecionar cepas de bactérias do ácido lático (BAL) para serem utilizadas como inoculantes e avaliar seus efeitos sobre a composição químico-bromatológica, a população de microrganismos, as perdas fermentativas e a estabilidade aeróbia de silagens de milho. No primeiro experimento, foi realizada seleção de BAL baseada no perfil de produção de metabólitos em extrato aquoso, taxas de crescimento e eficiência na redução do pH. As cepas que apre...

  11. Consorcio microbiano nativo con actividad catalítica para remoción de índigo y surfactantes en agua residual industrial textil a través de una matriz de inmovilización

    Directory of Open Access Journals (Sweden)

    Lliana Guerrero Porras

    2014-01-01

    Full Text Available El siguiente estudio tuvo como objetivo aislar seis diferentes cepas bacterianas  provenientes de las descargas de agua utilizadas en la tintura de hilo con colorante índigo, que tuviesen capacidad de degradación de compuestos orgánicos del tinte índigo y tres surfactantes de tipo no iónicos.  Igualmente, se evaluaron diferentes medios de soporte para inmovilizar las cepas seleccionadas. Las cepas con mejor capacidad de decoloración se combinaron para conformar cuatro consorcios (I, II, III, y IV con el fin de potenciar el proceso de decoloración, considerando que la sinergia y el complemento de actividades metabólicas de cultivos mixtos dentro de una comunidad microbiana incrementan la eficiencia de remoción de carga orgánica. Los porcentajes de remoción que se alcanzaron fueron 64, 73, 76 y 59 %, respectivamente. Los cultivos individuales no presentaron porcentajes de remoción superiores a los reportados por los consorcios, lo que permite pensar en su utilización para la remoción de tintes índigos en aguas residuales.

  12. Lesões cardíacas em ratos wistar inoculados com diferentes cepas do Trypanosoma cruzi

    Directory of Open Access Journals (Sweden)

    E. Chapadeiro

    1988-09-01

    Full Text Available Ratos albinos Wistar infectados com diferentes inóculos das cepas Y, Colombiana (COL e São Felipe (12SF do Trypanosoma cruzi desenvolveram parasitemia elevada entre 14e21 dias, a qual tomou-se desprezível ou desapareceu completamente ao final da 4ª semana. As lesões do coração foram avaliadas, semiquantitativamente, pelo sistema de "scores", conforme a intensidade e a extensão da inflamação, nas diferentes estruturas do órgão, tendo sido observado que: a na fase inicial (aguda da infecção, os animais mortos espontaneamente ou em conseqüência de acidente anestésico apresentaram cardite de intensidade moderada à acentuada, não havendo diferença em relação às cepas utilizadas; as diferentes estruturas do coração estavam uniformemente comprometidas; b na fase crônica da infecção observou-se cardite degrau leve a moderado e as lesões foram mais freqüentes e intensas quanto maior o inoculo empregado; no entanto, inóculos baixos só produziram lesões quando a cepa utilizada foi a Colombiana. Trombose mural nos átrios e dilatação da ponta do ventrículo esquerdo foram observadas em 20% dos animais, independentemente da cepa. Na fase crônica 15% dos animais apresentaram miocardite crônica com fibrose, associada à hipertrofia das miocélulas. Os AA confirmam ser o rato albino suscetível à infecção chagásica e, portanto, um modelo útil no estudo das manifestações cardíacas da Doença de Chagas.

  13. In Vitro Study of Berberis vulgaris, Actinidia deliciosa and Allium cepa L. Antibacterial Effects on Listeria monocytogenes

    OpenAIRE

    Anzabi Younes

    2015-01-01

    Objective: One control method of pathogenic microorganisms is using synthetic chemical preservatives and antibiotics. Because of being generally recognized as safe, antibacterial compounds with organic origin are considered important for health. This study was done in order to investigate the antibacterial effects of methanol extracts of the Berberis vulgaris (Barberry), Actinidia deliciosa (Kiwi) and Allium cepa L. (Onions) on the standard strain (ATCC:19114) of Listeria monocytogenes (L. mo...

  14. Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912 and Nyssomyia neivai (Pinto, 1926 (Diptera: Psychodidae

    Directory of Open Access Journals (Sweden)

    José Dilermando Andrade Filho

    2004-10-01

    Full Text Available The phlebotomine sand flies Nyssomyia intermedia (Lutz & Neiva, 1912 and Nyssomyia neivai (Pinto, 1926 are very close and may be involved in the transmission of Leishmania spp. Ross, 1903 in Brazil. The biology of the first laboratory-reared generations of these species, descended from insects captured in Além Paraíba (N. intermedia and Corinto (N. neivai in the Brazilian state of Minas Gerais, is described here. The captured females were fed on hamsters and maintained individually in rearing pots. Laboratory temperature and relative humidity were maintained at 25-26ºC and 80% respectively. The productivity of the first generation of N. intermedia was greater than that of N. neivai, and its development time clearly shorter, particularly for the second and third larval instars.

  15. Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae).

    Science.gov (United States)

    Andrade Filho, José Dilermando; Galati, Eunice A Bianchi; Falcão, Alda Lima

    2004-10-01

    The phlebotomine sand flies Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) are very close and may be involved in the transmission of Leishmania spp. Ross, 1903 in Brazil. The biology of the first laboratory-reared generations of these species, descended from insects captured in Além Paraíba (N. intermedia) and Corinto (N. neivai) in the Brazilian state of Minas Gerais, is described here. The captured females were fed on hamsters and maintained individually in rearing pots. Laboratory temperature and relative humidity were maintained at 25-26 masculineC and 80% respectively. The productivity of the first generation of N. intermedia was greater than that of N. neivai, and its development time clearly shorter, particularly for the second and third larval instars.

  16. Red blood cells free α-haemoglobin pool: a biomarker to monitor the β-thalassemia intermedia variability. The ALPHAPOOL study.

    Science.gov (United States)

    Vasseur, Corinne; Domingues-Hamdi, Elisa; Ledudal, Katia; Le Corvoisier, Philippe; Barau, Caroline; Ghaleh, Bijan; Rialland, Amandine; Pissard, Serge; Galactéros, Frédéric; Baudin-Creuza, Véronique

    2017-10-01

    The severity of β-thalassaemia (β-thal) intermedia is mainly correlated to the degree of imbalanced α/non α-globin chain synthesis. The phenotypic diversity of β-thal depends on this imbalance and reflects all possible combinations of α- and β-globin genotypes, levels of fetal haemoglobin (HbF) and co-inheritance of other modulating factors. This study aimed to demonstrate the validity of a new surrogate of α/non α-globin biosynthetic ratio by measuring the soluble α-Hb pool in lysed red blood cells. Our results confirm that the α-Hb pool measurement allows a good discrimination between β-thal intermedia patients, controls and α-thal patients (P intermedia patients and may be clinically useful for monitoring the evolution of the disequilibrium of globin synthesis in response to treatments. © 2017 John Wiley & Sons Ltd.

  17. Molecular Basis of β-Thalassemia Intermedia in Erbil Province of Iraqi Kurdistan.

    Science.gov (United States)

    Shamoon, Rawand P; Al-Allawi, Nasir A S; Cappellini, Maria D; Di Pierro, Elena; Brancaleoni, Valentina; Granata, Francesca

    2015-01-01

    β-Thalassemia intermedia (β-TI) is a clinical term describing a range of clinical phenotypes that are intermediate in severity between the carrier state and β-thalassemia major (β-TM). To characterize the molecular basis of β-TI in Erbil Province, Northern Iraq, 83 unrelated patients were investigated. Detection of β-globin gene mutations was carried out by reverse hybridization assay and direct gene sequencing. All patients were screened for the XmnI polymorphism by direct sequencing of HBG2 ((G)γ promoter gene). Detection of α-globin gene deletions and triplication was carried out using the reverse hybridization assay. Four main molecular patterns were identified in association with the β-TI phenotype, namely: β(+)/β(+) (38.5%), β(+)/β(0) (21.6%), β(0)/β(0) (31.3%), and β(0)/wild type (8.4%). IVS-I-6 (T > C) was the most frequently encountered mutation (55 alleles, 34.6%), followed by IVS-II-1 (G > A) and codon 8 (-AA); furthermore, we report for the first time from Iraq two β(+) mutations, -87 (C > G) and 5' untranslated region (5'UTR) +22 (G > A). The XmnI polymorphism was detected in 47.0% of patients, mainly in association with the β(0)/β(0) genotype. The α-globin gene deletions were encountered in four cases, including one case with (- -(FIL)) double gene deletion, a report that is the first from our country. The α-globin gene triplication was detected in five of the seven heterozygous β-thalassemia (β-thal) patients. Similar to other Mediterranean countries, inheritance of mild β-globin mutations was the main molecular pattern underlying β-TI in our patients followed by the ameliorating effect of the XmnI polymorphism.

  18. Hydroxyurea appears beneficial in patients with beta-thalassaemia major and intermedia

    Directory of Open Access Journals (Sweden)

    S. F.S.A. Abdul Wahid

    2007-06-01

    Full Text Available Patients with severe inherited β-globin chain disorders may have milder illness if they produce high levels of fetal hemoglobin (HbF. Hydroxyurea (HU has been shown to enhance HbF levels in patients with sickle cell disease and may be useful in β-thalassemias. We administered HU to 13 patients with β-thalassemia intermedia or major, including 6 splenectomized patients. The patients received escalating doses (10 to 25 mg/kg/d of HU for around 2 years (median: 21 months, range: 8 - 55 months. Eleven patients responded with an increase in the pre-transfusion HbF levels, from a base line median of 8.0% (2.5 - 61.3% to 28.0% (6.6 - 49.2% and 40.7% (4.8 - 72.3% at 3 months and 18 months post-HU, respectively. A concomitant increment in median hemoglobin levels was noted at 1, 3 and 18 months of HU therapy. Six of 7 transfusion-dependent patients who had an increment of HbF (one with β-thalassemia major also had reduced transfusion requirement over the 2-year period of HU therapy. Response to HU was also shown by a reduction in spleen size. Apart from oral ulcers that resolved upon dose reduction of HU, no significant toxicity was noted. We conclude that increased HbF production in β-thalassemia patients, with an improvement in erythropoiesis, can be achieved using HU with minimal toxicity. (Med J Indones 2007; 16:78-83 Keywords: fetal hemoglobin (HbF, erythropoiesis, toxicity

  19. A Novel Hyaluronidase from Brown Spider (Loxosceles intermedia) Venom (Dietrich's Hyaluronidase): From Cloning to Functional Characterization

    Science.gov (United States)

    Ferrer, Valéria Pereira; de Mari, Thiago Lopes; Gremski, Luiza Helena; Trevisan Silva, Dilza; da Silveira, Rafael Bertoni; Gremski, Waldemiro; Chaim, Olga Meiri; Senff-Ribeiro, Andrea; Nader, Helena Bonciani; Veiga, Silvio Sanches

    2013-01-01

    Loxoscelism is the designation given to clinical symptoms evoked by Loxosceles spider's bites. Clinical manifestations include skin necrosis with gravitational spreading and systemic disturbs. The venom contains several enzymatic toxins. Herein, we describe the cloning, expression, refolding and biological evaluation of a novel brown spider protein characterized as a hyaluronidase. Employing a venom gland cDNA library, we cloned a hyaluronidase (1200 bp cDNA) that encodes for a signal peptide and a mature protein. Amino acid alignment revealed a structural relationship with members of hyaluronidase family, such as scorpion and snake species. Recombinant hyaluronidase was expressed as N-terminal His-tag fusion protein (∼45 kDa) in inclusion bodies and activity was achieved using refolding. Immunoblot analysis showed that antibodies that recognize the recombinant protein cross-reacted with hyaluronidase from whole venom as well as an anti-venom serum reacted with recombinant protein. Recombinant hyaluronidase was able to degrade purified hyaluronic acid (HA) and chondroitin sulfate (CS), while dermatan sulfate (DS) and heparan sulfate (HS) were not affected. Zymograph experiments resulted in ∼45 kDa lytic zones in hyaluronic acid (HA) and chondroitin sulfate (CS) substrates. Through in vivo experiments of dermonecrosis using rabbit skin, the recombinant hyaluronidase was shown to increase the dermonecrotic effect produced by recombinant dermonecrotic toxin from L. intermedia venom (LiRecDT1). These data support the hypothesis that hyaluronidase is a “spreading factor”. Recombinant hyaluronidase provides a useful tool for biotechnological ends. We propose the name Dietrich's Hyaluronidase for this enzyme, in honor of Professor Carl Peter von Dietrich, who dedicated his life to studying proteoglycans and glycosaminoglycans. PMID:23658852

  20. Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR.

    Science.gov (United States)

    Gomes, B P F A; Jacinto, R C; Pinheiro, E T; Sousa, E L R; Zaia, A A; Ferraz, C C R; Souza-Filho, F J

    2005-08-01

    he aim of this study was to investigate the presence of four black-pigmented bacteria, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens, in endodontic infections by culture and polymerase chain reaction (PCR) analyses. Microbial samples were obtained from 50 teeth with untreated necrotic pulps (primary infection) and from 50 teeth with failing endodontic treatment (secondary infection). Microbiological strict anaerobic techniques were used for serial dilution, plating, incubation, and identification. For PCR detection, the samples were analyzed using species-specific primers of 16S rDNA and the downstream intergenic spacer region. Culture and PCR detected the test species in 13/100 and 50/100 of the study teeth, respectively. The organisms were cultured from 11/50 (22%) of primarily infected root canal samples and from 2/50 (4%) of secondary root canal samples. PCR detection identified the target species in 32/50 (64%) and 18/50 (36%) of primary and secondary infections, respectively. P. gingivalis was rarely isolated by culture methods (1%), but was the most frequently identified test species by PCR (38%). Similarly, P. endodontalis was not recovered by culture from any tooth studied, but was detected by PCR in 25% of the sampled teeth. PCR-based identification also showed higher detection rates of P. intermedia (33%) and P. nigrescens (22%) than culture (13%). In conclusion, P. gingivalis, P. endodontalis, P. intermedia, and P. nigrescens were identified more frequently in teeth with necrotic pulp than in teeth with failing endodontic treatment. Also, a higher frequency of black-pigmented species was detected by PCR than by culture.

  1. EFECTO DE LA MICORRIZA Y GALLINAZA SOBRE LA PRODUCCIÓN Y LA CALIDAD DE CEBOLLA CABEZONA (Allium cepa L. ‘Yellow Granex’ EFFECT OF MYCORRHIZAE AND HEN MANURE FERTILIZATION ON YIELD AND QUALITY OF ONION (Allium cepa L. ‘YELLOW GRANEX’

    Directory of Open Access Journals (Sweden)

    Maritza Yolima Agudelo Becerra

    2004-06-01

    Full Text Available En condiciones de campo se evaluó el efecto de hongos micorrizógenos y de gallinaza, comparado con la aplicación de fertilizante mineral, sobre la producción y la calidad de cebolla cabezona (Allium cepa L. ‘Yellow Granex’ en el municipio de Cucaita - Boyacá (Colombia. Las semillas germinaron en suelo, y 70 días después de la germinación, se seleccionaron plántulas de tamaño uniforme, se transplantaron y se inocularon (0,86 ton/ha de Mycofertil o no con hongos micorrícicos. Se aplicaron tres dosis de gallinaza (0 - 1 y 2 ton/ha y 0,5 ton / ha de fertilizante comercial 15 - 15 - 15. Se evaluó la producción total, la producción de bulbos de primera y segunda calidad, así como de los bulbos dañados. Se utilizó el diseño de bloques al azar con tres replicaciones. Se hizo un análisis de varianza (ANOVA para los datos registrados y se realizó la prueba de comparación de medias de Duncan con un nivel de significancia de 0,01. Los resultados mostraron que las micorrizas y la gallinaza incrementaron la producción total y de las cebollas de primera calidad, comparados con la aplicación fertilizante mineral solo o complementado con 1 ó 2 ton/ha de gallinaza. Sin embargo, la producción de cebolla de segunda calidad fue superior en las parcelas tratadas con micorrizas, comparadas con los otros tratamientos sin ellas. Por otro lado, la adición de micorrizas redujo la cantidad de bulbos dañados. El tratamiento con fertilizante mineral solo presentó la producción total más baja y la menor producción de cebolla de primera y segunda calidad, pero la más alta producción de cebollas dañadas, en comparación con los demás tratamientos evaluados. Los resultados se discutieron con base en el incremento en la toma de nutrientes y en la reducción del efecto de patógenos radiculares en las plantas colonizadas con micorrizas.Under field conditions, the effects of mycorrhizae and hen manure fertilization, compared to the application

  2. Control of Botrytis cinerea in Eucalyptus globulus Mini-Cuttings Using Clonostachys and Trichoderma Strains Control de Botrytis cinerea en miniestacas de Eucalyptus globulus Utilizando Cepas de Clonostachys y Trichoderma

    Directory of Open Access Journals (Sweden)

    Salomé Zaldúa

    2010-12-01

    . globulus. Se probaron cinco cepas de hongos, dos de Clonostachys y tres de Trichoderma (5 x 10(6 conidias mL-1 aplicados semanalmente. Además, se utilizaron tratamientos para realizar comparaciones: control absoluto (agua y aplicación de fungicidas. El ensayo se realizó bajo condiciones operacionales de producción de mini-estacas de E. globulus. Las cepas de Clonostachys UDC-A10 y UDC-A11 redujeron la mortalidad de las mini-estacas ocasionada por B. cinerea en 54% y 71%, respectivamente, con efectos similares a los logrados por los fungicidas. Clonostachys UDC-A11 redujo la tasa de incremento de la enfermedad con resultados estadísticamente similares a los obtenidos con fungicidas. Además, con la aplicación de fungicidas se constató un efecto negativo en el enraizamiento de las mini-estacas sobrevivientes. Estos resultados demuestran la eficacia de Clonostachys como agente de control contra la enfermedad del moho gris en mini-estacas de E. globulus.

  3. Evaluation of DNA by the micronucleus test of the samples Allium cepa germinated in the presence of uranium

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Ayrton Nascimento de; Oliveira, Andressa L.; Gennari, Roseli F.; Maffei, Eliane M.D.; Campos, Simara S., E-mail: simaracampos@gmail.com [Universidade Estadual do Sudoeste da Bahia (UESB), Vitoria da Conquista, BA, (Brazil). Departamento de Ciencias Exatas e Tecnologicas; Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Instituto de Fisica. Departamento de Fisica Nuclear

    2017-11-01

    Although Brazil composes the seventh position in the world ranking of uranium reserves, the ore is still little explored in Brazil, considering its vast existing arsenal. On the one hand, despite nuclear energy has brought great benefits, technological and socio-economic development, it generates controversy about environmental contamination and risks to public health. Studies on this subject indicate that areas where uranium ore concentration is high, natural environmental radiation exposure levels are already higher than in other regions. The aim of this study is to observe the simple germination of the bio-indicator (Allium cepa), typically used to assess potential chromosomal aberrations, suffer any adverse effect caused by natural radiation uranium. The choice of this bio-indicator is based on its potential for evaluating the mutation caused by innumerous chemical compounds. Four treatments with three replicates were designed. In each treatment, 10 seeds of onion A. cepa without any pesticides were packed in the Petri dish lined with germination paper and room temperature (25°C) was kept until the root reaches approximately 1cm long and 0, 5 g of the U. As a result 3000 cells were counted during the interphase, and structural changes were observed in the chromosomes, elongated nuclei and loose chromosomes of the spindle fiber, concluding that U possibly affected the cell cycle da Allium cepa. (author)

  4. The diversity and abundance of small arthropods in onion, Allium cepa, seed crops, and their potential role in pollination.

    Science.gov (United States)

    Walker, M K; Howlett, B G; Wallace, A R; McCallum, J A; Teulon, D A J

    2011-01-01

    Onion, Allium cepa L. (Asparagales: Amaryllidaceae), crop fields grown for seed production require arthropod pollination for adequate seed yield. Although many arthropod species visit A. cepa flowers, for most there is little information on their role as pollinators. Small flower visiting arthropods (body width arthropods were highly abundant among all except one field. Insects belonging to the orders Diptera and Thysanoptera were the most abundant and Hymenoptera, Collembola, Psocoptera, Hemiptera, and Coleoptera were also present. To test whether small arthropods might contribute to pollination, seed sets from umbels caged within 3 mm diameter mesh cages were compared with similarly caged, hand-pollinated umbels and uncaged umbels. Caged umbels that were not hand-pollinated set significantly fewer seeds (average eight seeds/umbel, n = 10) than caged hand-pollinated umbels (average 146 seeds/umbel) and uncaged umbels (average 481 seeds/umbel). Moreover, sticky traps placed on umbels within cages captured similar numbers of small arthropods as sticky traps placed on uncaged umbels, suggesting cages did not inhibit the movement of small arthropods to umbels. Therefore, despite the high abundance of small arthropods within fields, evidence to support their role as significant pollinators of commercial A. cepa seed crops was not found.

  5. Efficacy of Allium cepa test system for screening cytotoxicity and genotoxicity of industrial effluents originated from different industrial activities.

    Science.gov (United States)

    Pathiratne, Asoka; Hemachandra, Chamini K; De Silva, Nimal

    2015-12-01

    Efficacy of Allium cepa test system for screening cytotoxicity and genotoxicity of treated effluents originated from four types of industrial activities (two textile industries, three rubber based industries, two common treatment plants of industrial zones, and two water treatment plants) was assessed. Physico-chemical parameters including the heavy metal/metalloid levels of the effluents varied depending on the industry profile, but most of the measured parameters in the effluents were within the specified tolerance limits of Sri Lankan environmental regulations for discharge of industrial effluents into inland surface waters. In the A. cepa test system, the undiluted effluents induced statistically significant root growth retardation, mitosis depression, and chromosomal aberrations in root meristematic cells in most cases in comparison to the dilution water and upstream water signifying effluent induced cytotoxicity and genotoxicity. Ethyl methane sulphonate (a mutagen, positive control) and all the effluents under 1:8 dilution significantly induced total chromosomal aberrations in root meristematic cells in comparison to the dilution water and upstream water indicating inadequacy of expected 1:8 dilutions in the receiving waters for curtailing genotoxic impacts. The results support the use of a practically feasible A. cepa test system for rapid screening of cytotoxicity and genotoxicity of diverse industrial effluents discharging into inland surface waters.

  6. Evaluation of DNA by the micronucleus test of the samples Allium cepa germinated in the presence of uranium

    International Nuclear Information System (INIS)

    Souza, Ayrton Nascimento de; Oliveira, Andressa L.; Gennari, Roseli F.; Maffei, Eliane M.D.; Campos, Simara S.; Universidade de Sao Paulo

    2017-01-01

    Although Brazil composes the seventh position in the world ranking of uranium reserves, the ore is still little explored in Brazil, considering its vast existing arsenal. On the one hand, despite nuclear energy has brought great benefits, technological and socio-economic development, it generates controversy about environmental contamination and risks to public health. Studies on this subject indicate that areas where uranium ore concentration is high, natural environmental radiation exposure levels are already higher than in other regions. The aim of this study is to observe the simple germination of the bio-indicator (Allium cepa), typically used to assess potential chromosomal aberrations, suffer any adverse effect caused by natural radiation uranium. The choice of this bio-indicator is based on its potential for evaluating the mutation caused by innumerous chemical compounds. Four treatments with three replicates were designed. In each treatment, 10 seeds of onion A. cepa without any pesticides were packed in the Petri dish lined with germination paper and room temperature (25°C) was kept until the root reaches approximately 1cm long and 0, 5 g of the U. As a result 3000 cells were counted during the interphase, and structural changes were observed in the chromosomes, elongated nuclei and loose chromosomes of the spindle fiber, concluding that U possibly affected the cell cycle da Allium cepa. (author)

  7. α:Non–α and Gγ:Aγ globin chain ratios in thalassemia intermedia patients treated with hydroxyurea

    Directory of Open Access Journals (Sweden)

    Abbas Najjari

    2014-05-01

    Conclusions: Improvement in α:non-α ratio and consequent decrease of free α-globin chain might be the cause of beneficial effects of hydroxyurea therapy. Two patients who felt better didn't show significant increase in their fetal hemoglobin level, and this is in contradiction with the hypothesis claiming that the HbF level increase is the cause of such therapeutic effect. In spite of the unclear mechanism of action of this drug, hydroxyurea therapy had noticeable impacts on thalassemia intermedia and also sickle cell disease and even patients suffering from thalassemia major.

  8. Effect of Allium cepa and Allium sativum on some immunological cells in rats.

    Science.gov (United States)

    Mirabeau, Tatfeng Y; Samson, Enitan S

    2012-01-01

    Extracts of some spices have been reported to play a contributory role in enhancing immune function. We evaluated and compared the effect(s) of single and combined oral administration of fresh aqueous onion (Allium cepa) and garlic (Allium sativum) extracts at different concentrations on some immunological determinants in rats. CD₄ cells of the rats were estimated using Partec flow cytometric technique, while total and differential white blood cell (WBC) counts were estimated using the Sysmsex® automated haematology analyzing technique. Our findings revealed that, CD4 and total WBC counts were significantly increased (P≤0.05) in a dose-dependent manner in both onion (250mg/Kg/d: 349±11cell/ul and 2.75±0.15X10³cell/l; 500mg/Kg/d: 389±10cells/µl and 3.05±0.05 X10³cell/l; 750mg/Kg/d: 600±11cell/µl and 3.25±0.05X10³cells/l) and garlic (250mg/Kg/d: 410±10cell/ul and 2.85±0.15X10³cell/l; 500mg/Kg/d: 494±32cells/µl and 3.30±0.10 X10³cell/l; 750mg/Kg/d: 684±11cell/µl and 3.55±0.05X10³cells/l) treated rats when compared to the zero control (200±11cells/µl and 1.55±0.05X10³cells/l, respectively). Extract of garlic at 750mg/Kg/d had significantly increased the CD4 cells and total white cell count when compared to other concentrations (P≤0.05). However, no significant effect was observed on these parameters when extracts were combined (250mg/Kg/d: 252±21cell/µl and 1.80±0.10X10³cells/l; 500mg/Kg/d: 315±21cells/ul and 2.10±0.10X10³cells/l; 750mg/Kg/d: 368±10cells/µl and 2.35±0.05X10³cells/l, respectively), the differential WBC count showed a significant increase in the proportion of cell types (lymphocytes, neutophils and monocytes) (P≤0.05). The results from this study revealed the immune boosting capabilities of Allium cepa and Allium sativum, but underscored their synergistic activities.

  9. A methodology for determining environmental threshold quantities for substances covered by CEPA's Environmental Emergency Regulation

    International Nuclear Information System (INIS)

    Ketcheson, K.; Shrives, J.

    2005-01-01

    Sections 199 and 200 of the Canadian Environmental Protection Act (CEPA) 1999 oblige persons who own or manage specified toxic and hazardous substances to develop and implement environmental emergency plans. This paper discussed the methodology for determining how a chemical is assessed for recommending an environmental emergency plan. For Section 199, once substances are declared toxic, each chemical is assessed to determine whether it requires a plan or not. For Section 200, any chemical can be added under the E2 regulations, as long as it can be ascertained that the substance is toxic according to the following criteria: it has an immediate or long-term harmful effect on the environment or its biological diversity; it constitutes a danger to the environment on which human life depends; and/or it constitutes a danger in Canada to human life or health. An overview of the risk evaluation framework was provided, including details of the pre-assessment filter. Summaries of trigger criteria were presented, as well as environmental hazard ratings and details of persistence of organic chemicals in the environment and bioaccumulation. Aquatic toxicity and ingestion toxicity details were also provided. Human hazard ratings included carcinogenicity, inhalation toxicity, dermal toxicity, rabbit and rat toxicity and corrosion and skin irritation ratings. Issues concerning vapour cloud explosions were examined. A reactivity table was presented with hazard descriptions. European Union Threshold quantities were examined, as well as a list of comparisons of selected substances of CEPA with the European Union. It was concluded that the Environmental Emergency Branch (EEB) has created environmental thresholds by first examining how other countries have protected the environment. Substance thresholds for the United States have focused on protecting humans, while Europe has established threshold quantities that work for their countries. The EEB has selected classification tables

  10. Diferenciação de cepas de Candida albicans pelo sistema killer

    Directory of Open Access Journals (Sweden)

    Regina Celia Cândido

    1995-12-01

    Full Text Available Foi estudado o efeito killer de 9 cepas padrão de leveduras sobre 146 amostras de Candida albicans isoladas dos seguintes espécimes clínicos: mucosa bucal, fezes, lavado brônquico, escarro, secreção vaginal, urina, lesão de pele, lesão de unha e sangue. Usando este sistema foi possível diferenciar 23 biotipos de C. albicans. Os biotipos 211, 111 e 811 foram os mais freqüentemente isolados. A maioria das amostras de C. albicans (98,6% foi sensível a pelo menos uma ou mais das 9 cepas killer. Empregando- se este sistema foi possível demonstrar que 2 pacientes albergavam mesmo biotipo killer, respectivamente, 111 e 211, em diferentes espécimes clínicos, e em outro paciente, o mesmo biotipo (211 foi isolado de hemoculturas realizadas em ocasiões distintas. O uso do sistema killer para diferenciar os tipos entre as espécies de leveduras patogênicas, pode ser um método útil para estabelecer a eventual fonte de infecção, constituindo uma ajuda valiosa para o controle e vigilância de infecções nosocomiais causadas por leveduras.The authors studied the killer effect of nine standard strains of yeasts on 146 samples of Candida albicans isolated from the following clinical specimens: oral mucosa, feces, bronchial wash, sputum, vaginal secretion, urine, skin lesion, nail lesion and blood. Using this system it was possible to differentiate 23 biotypes of Candida albicans. The biotypes 211, 111 and 811 were most frequently isolated. Most of the samples of C. albicans (98.6% were sensitive to at least one or more of the nine killer strains. Using the killer system it was possible to show that two patients harbored the same killer biotypes, 111 and 211, respectively, in different clinical specimens and another patient harbored the same biotype (211 in blood cultures effected in different ocasions. The utilization of the killer system to differentiate types among species of pathogenic yeasts can be a useful method to stablish the eventual

  11. Characteristics of β-lactamases and their genes (blaA and blaB in Yersinia intermedia and Y. frederiksenii

    Directory of Open Access Journals (Sweden)

    Sharma Sachin

    2007-04-01

    Full Text Available Abstract Background The presence of β-lactamases in Y. enterocolitica has been reported to vary with serovars, biovars and geographical origin of the isolates. An understanding of the β-lactamases in other related species is important for an overall perception of antibiotic resistance in yersiniae. The objective of this work was to study the characteristics of β-lactamases and their genes in strains of Y. intermedia and Y. frederiksenii, isolated from clinical and non-clinical sources in India. Results The enzymes, Bla-A (a constitutive class A penicillinase and Bla-B (an inducible class C cephalosporinase were found to be present in all the clinical and non-clinical strains of Y. intermedia and Y. frederiksenii by double disc diffusion method. The results showed differential expression of Bla-A as indicated by presence/absence of synergy whereas expression of Bla-B was quite consistent. The presence of these enzymes was also reflected in the high minimum inhibitory concentrations, MIC50 (126–1024 mg/L and MIC90 (256–1024 mg/L of β-lactam antibiotics against these species. Restriction fragment length polymorphism (RFLP revealed heterogeneity in both blaA and blaB genes of Y. intermedia and Y. frederiksenii. The blaA gene of Y. intermedia shared significant sequence identity (87–96% with blaA of Y. enterocolitica biovars 1A, 1B and 4. The sequence identity of blaA of Y. frederiksenii with these biovars was 77–79%. The sequence identity of blaB gene of Y. intermedia and Y. frederiksenii was more (85% with that of Y. enterocolitica biovars 1A, 1B and 2 compared to other species viz., Y. bercovieri, Y. aldovae and Y. ruckeri. Isoelectric focusing data further revealed that both Y. intermedia and Y. frederiksenii produced Bla-A (pI 8.7 and "Bla-B like" (pI 5.5–7.1 enzymes. Conclusion Both Y. intermedia and Y. frederiksenii showed presence of blaA and blaB genes and unequivocal expression of the two β-lactamases. Limited heterogeneity

  12. Paciente con esquizofrenia tratado con ziprasidona + clozapina

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    Pol Yanguas E.

    2013-05-01

    Full Text Available P es un paciente diagnosticado de esquizofrenia, sigue en un piso tutelado un programa de rehabilitación, está medicado con clozapina 500 mg/día y ziprasidona 280 mg/ día. Padece hipercolesterolemia, tabaquismo y sus hábitos alimenticios no son buenos. La medicación que utiliza desde 2007 hasta ahora se refleja en la tabla 1. El último tratamiento se le introdujo el 7 de agosto de 2012, habiendo presentado un electro cardiograma (ECG normal, pero con ligera taquicardia ventricular y prolactinemia de 44,8 ng/ml (valores normales: 2-18 ng/ml.

  13. Optimización de la transformación genética con Agrobacterium rhizogenes

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    Mónica Blanco

    2003-01-01

    Full Text Available Para optimizar la eficiencia en la transformación genética con Agrobacterium rhizogenes, secciones de zanahoria fueron inoculadas con la cepa de Agrobacterium A4TC y co-cultivadas con acetosiringona, floroglucinol y una combinación de ambos. La acetosiringona es uno de los compuestos fenólicos que liberan los tejidos vegetales como respuesta a heridas, el cual induce la transferencia del T-ADN de la agrobacteria a la planta. El floroglucinol es también un compuesto fenólico; sin embargo, ejerce un efecto sinergístico con las auxinas, inhibiendo parcialmente la actividad de las citocininas. La mayor eficiencia en la transformación (75% se obtuvo con el uso de acetosiringona (100 mM en combinación con floroglucinol (25 mg l-1. En general, 6 días de co-cultivo, independientemente de los tratamientos, fue el tiempo que indujo el mayor porcentaje de transformación. La inclusión de 100 mg l-1 de kanamicina resultó un mecanismo eficiente para discriminar entre raíces transformadas y no transformadas. En adición, este trabajo presenta un novedoso sistema con el cual, una vez realizada la transformación, la bacteria puede ser eliminada completamente en 48 h con Cefotaxime en dosis de 500 mg l-1.

  14. Análisis genómico del resistoma de la cepa de Acinetobacter baumannii ABIBUN 107m multi-resistente y persistente en hospitales colombianos

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    Maria Teresa Reguero

    2014-07-01

    Full Text Available Título en español: Análisis genómico del resistoma de la cepa  de  Acinetobacter baumannii  ABIBUN 107m multi-resistente  y persistente  en hospitales colombianos Título en ingles: Genomic analysis of the resistoma of the strain of Acinetobacter baumannii ABIBUN 107m multi-resistant and persistent in Colombian hospitals Título corto: Análisis genómico del resistoma de la cepa  de  Acinetobacter baumannii  Resumen: Acinetobacter baumannii es una bacteria, causante de infecciones asociadas a la atención en salud como neumonía, septicemia, meningitis e infecciones urinarias entre otras. Se caracteriza por su capacidad para desarrollar y acumular rápidamente una gran variedad de mecanismos de resistencia a antibióticos. En esta investigación se realizó el análisis genómico de una cepa de A. baumannii ABIBUN 107m que forma parte de un clon persistente en hospitales colombianos, resistente a los antibióticos carbapenémicos (imipenem y meropenem,  antibióticos de elección en el tratamiento  infecciones causadas por este microorganismo. El genoma de esta bacteria fue secuenciado utilizando técnicas de alto rendimiento, ensamblado y anotado, obteniéndose un genoma constituido por  3954000 pb con 56 contigs;  consta de 4256 genes con un tamaño promedio de 912 pb;  3796 CDS  de los cuales por anotación 2884  se asignaron a COG; 57 tRNA y un porcentaje de GC de 38,74%. A. baumannii ABIBUN 107m es resistente a b-lactámicos, aminoglicósidos, quinolonas, tetraciclina, sulfonamida y colistina. En su genoma se localizaron genes asociados con el perfil de resistencia ya que presenta serin b-lactamasas (blaADC-38, blaOXA-64, blaOXA-23, blaampC-like,  blaamp(H-like, metalo b-lactamasa_B;  proteínas de unión a  penicilina de elevada masa molecular, secuencias de inserción tipo ISAba1; mutaciones en los genes de DNA girasa  y topoisomerasa IV subunidad A (gyrA y parC; enzimas modificadoras de aminoglicósidos (aph

  15. Portadores nasofaríngeos de Neisseria meningitidis en trabajadores con riesgo ocupacional

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    Isabel Martínez

    2010-04-01

    Full Text Available Los portadores de Neisseria meningitidis constituyen la principal fuente de infección y transmisión de la enfermedad meningocócica. Conocer su prevalencia, las características de las cepas aisladas y los factores de riesgos asociados con el estado de portador, aportan datos valiosos al control y vigilancia epidemiológica de esta entidad clínica. Para cumplimentar los objetivos propuestos se realizó un estudio transversal descriptivo de portadores de N. meningitidis en 112 trabajadores de un centro de producción de biofarmacéuticos de La Habana, con edades comprendidas entre 18_60 años. Previo a su realización se cumplió con las exigencias bioéticas requeridas para este tipo de estudio. A todos se les realizó un exudado nasofaríngeo y una encuesta, donde se indagó sobre factores de riesgo (edad, sexo, hacinamiento, hábito de fumar, consumo de bebidas alcohólicas, amigdalectomía y antecedentes de infección respiratoria que favorecen la condición del portador. La identificación de las cepas de N. meningitidis se realizó según métodos convencionales, la clasificación de los serogrupos se hizo por aglutinación en láminas portaobjetos con antisueros comerciales y para la identificación de los serotipos y subtipos se empleó un ensayo inmunoenzimático (ELISA de células enteras con anticuerpos monoclonales. Se detectó un 8% de portadores de N. meningitidis con predominio del serogrupo B (77,8% y el fenotipo más frecuente fue el B:4:P1.4 (33,3%. Al analizar el estado de portador y su asociación con los factores de riesgo, la edad (p = 0,05 y el sexo (p = 0,013 mostraron diferencias significativas. Se demostró la posibilidad del riesgo ocupacional en aquellos individuos que por su profesión están en contacto con microorganismos patógenos

  16. Induction of cytotoxic and genotoxic effects of Guandu River waters in the Allium cepa system

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    Jennifer Vieira Gomes

    2015-01-01

    Full Text Available The Guandu River is the main source of water supply for the metropolitan region of Rio de Janeiro and has been facing serious environmental problems due to increasing population and industrial pollution, as well as the presence of polluted tributaries. This study analyzed the cytotoxic and genotoxic potential of the Guandu River’s waters, through the use of the Allium cepa test system. Collection points were chosen at the greatest confluences of pollutant sources. The sampling included two different seasons: the rainy season (January and February and the dry season (June and July. The analyses of 5000 cells per treatment showed that all the points studied had some degree of cytotoxicity and/or genotoxicity. Two sampling locations, which receive major influxes from the polluted waters of the Poços/Queimados and Cabuçu/Ipiranga Rivers, stood out for the strong presence of micronuclei, sticky chromosomes, mitotic spindle abnormalities, necrotic cells and nucleolar changes compared to the negative control. At least two locations also found changes in the mitotic index. The existence of variations in the number of cytotoxic and genotoxic changes between periods of rain and drought indicates that the cytotoxic and genotoxic potential of the water pollutants varies according to time, depending on the discharges of the tributary rivers and the increase of contaminated effluents. The results highlight the importance of bio-monitoring to assist managers in the control of effluent discharge.

  17. Evaluation of the cytogenotoxicity of textile effluents using Allium cepa L.

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    Sandro Barbosa

    2011-08-01

    Full Text Available The cytotoxic and genotoxic potential of the raw (EB and treated (ET effluents of two textile mills located in south of Minas Gerais State that have their effluents treated at the same Effluent Treatment Plant was investigated using the Allium cepa test system. Cytotoxicity was evaluated by the root elongation and mitotic index (MI endpoints and the genotoxicity was assessed by de determination of chromosome aberrations (CA.The effluent samples were tested at the concentrations 0 (ultrapure water, 25, 50, 75, and 100 % (v/v. A Completely Randomized Design with four replicates of 30 seeds was used. The effluent samples in almost all tested concentrations promoted an increase in root elongation compared to the negative control and this effect was probably related to nutrients levels and organic matter in effluent samples. A lower MI at all concentrations of ET compared to EB. The highest MI was observed at 100% (v/v concentration of both effluents. The highest rates of CA occurred at concentrations 75% (v/v of EB and 100% (v/v of both effluents. The effluent samples showed no cytotoxic effect, but cell division occurred disorderly, leading to increase rate of AC, revealing a genetoxic effect. Improvements in the wastewater treatment are needed to reduce environmental impacts.

  18. Detection irradiated shallot (Allium Cepa) and spices using technique of Photo Stimulated Luminescence (PSL)

    International Nuclear Information System (INIS)

    Noor Azianti Abd Rashid

    2012-01-01

    This study was carried out to investigate the effectiveness of Photo Stimulated Luminescence (PSL) in detecting the food that has been irradiated qualitatively and quantitatively. The samples used were onions (Allium Cepa) and spices. The shallots which are from India and Thailand was irradiated using Gamma Cell at dose of 0 kGy, 0.2 kGy, 0.4 kGy and 1.0. While for the spices, there were 10 types of spices used such as powder of paprika, parsley, turmeric, tarragon, coriander, mint leaves, basil, rosemary flower, chives and herbs mixture which was irradiated at the dose of 0 kGy and 8.7 kGy respectively. The result shows that the PSL method could differentiate samples which are irradiated and not irradiated. Even though the PSL value increases proportionally to the radiation dose, the relationship between these two means are not that clearly clarified and needed to be studied furthermore.(author)

  19. Dichlorophen and Dichlorovos mediated genotoxic and cytotoxic assessment on root meristem cells of Allium cepa

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    Sibhghatulla Shaikh

    2012-06-01

    Full Text Available Plants are direct recipients of agro – toxics and therefore important materials for assessing environmental chemicals for genotoxicity. The meristematic mitotic cell of Allium cepa is an efficient cytogenetic material for chromosome aberration assay on environmental pollutants. Onion root tips were grown on moistened filter paper in petri dish at room temperature. Germinated root tips were then exposed to three concentrations of each pesticide for 24 h. About 1 – 2 mm length of root tip was cut, fixed in cornoy’s fixative, hydrolyzed in warm 1 N HCL, stained with acetocarmine and squashed on glass slide. About 3000 cells were scored and classified into interphase and normal or aberrant division stage. Cytotoxicity was determined by comparing the mitotic index (MI of treated cells with that of the negative control. The MI of cells treated with Dichlorophen and Dichlorovos at one or more concentration was half or less than that of control are said to be cytotoxic. Genotoxicity was measured by comparing the number of cells/1000 in aberrant division stages at each dose with the negative control using Mann – Whitney U test. Both Dichlorophen and Dichlorovos are genotoxic at higher concentrations i.e. 0.001%, 0.002% and 0.028%, 0.056% inducing chromosome fragment, chromosome lagging and bridges, stick chromosome and multipolar anaphase.

  20. Structural analyses and immunomodulatory properties of fructo-oligosaccharides from onion (Allium cepa).

    Science.gov (United States)

    Kumar, V Prasanna; Prashanth, K V Harish; Venkatesh, Y P

    2015-03-06

    Onion (Allium cepa) is an immune-boosting food rich in fructans. The major aim of this study is to characterize and investigate the immunomodulatory properties of onion fructo-oligosaccharides (FOS). FOS was isolated from onion bulbs by hot 80% ethanol extraction (yield: ∼4.5 g/100 g fw) followed by gel permeation chromatography. NMR of onion FOS revealed unusual β-D-Glc terminal residue at the non-reducing end. TLC and ESI-MS analyses showed that onion FOS ranged from trisaccharides to hexasaccharides. Onion FOS (50 μg/mL) significantly increased (∼3-fold) the proliferation of mouse splenocytes/thymocytes vs. control. Further, onion FOS enhanced (∼2.5-fold) the production of nitric oxide by peritoneal exudates cells (PECs) from Wistar rats; intracellular free radicals production and phagocytic activity of isolated murine PECs were also augmented. Our structural and in vitro results indicate that onion FOS comprising of tri- to hexasaccharide units belongs to inulin-type fructans, and possess immunostimulatory activities towards murine lymphocytes and macrophages. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Effect of different exposed lights on quercetin and quercetin glucoside content in onion (Allium cepa L.).

    Science.gov (United States)

    Ko, Eun Young; Nile, Shivraj Hariram; Sharma, Kavita; Li, Guan Hao; Park, Se Won

    2015-07-01

    Quercetin and quercetin glucosides are the major flavonols present in onion (Allium cepa L.) and are predominantly present as quercetin, quercetin-3,4'-diglucoside and quercetin-4'-glucoside. Effect of different light wavelengths on onion after harvest and storage, with fluorescent, blue, red and ultra violet light influenced the quercetin and quercetin glucosides profile. In a peeled onion, all the light treatments elevated quercetin content in bulb. Among them, particularly fluorescent light effect was more eminent which stimulates the maximum synthesis of quercetin in onion. In case of whole onion bulb, skin and pulp showed different responses to light treatment, respectively. The pulp had the highest quercetin glucosides under blue light, whereas the lowest under fluorescent light. Onion skin showed nearly opposite pattern as compared to the pulp. In particular, light treatment proved to be a better way to increase the level of quercetin content in onions which might be utilized for industrial production of bioactive compounds from onion and onion waste products.

  2. Phytotoxicity of pesticides mancozeb and chlorpyrifos: correlation with the antioxidative defence system in Allium cepa.

    Science.gov (United States)

    Fatma, Firdos; Verma, Sonam; Kamal, Aisha; Srivastava, Alka

    2018-02-01

    Pesticides are a group of chemical substances which are widely used to improve agricultural production. However, these substances could be persistent in soil and water, accumulative in sediment or bio-accumulative in biota depending on their solubility, leading to different types of environmental pollution. The present study was done to assess the impact of pesticides-mancozeb and chlorpyrifos, via morphological and physiological parameters using Allium cepa test system. Phytotoxic effects of pesticides were examined via germination percentage, survival percentage, root and shoot length, root shoot length ratio, seedling vigor index, percentage of phytotoxicity and tolerance index. Oxidative stress on Allium seedlings caused by pesticides was also assessed by investigating the activity of antioxidative enzymes viz. catalase, peroxidase and superoxide dismutase. Correlation was worked out between morphological parameters and antioxidative enzymes to bring out the alliance between them. Mancozeb and chlorpyrifos concentrations were significantly and positively correlated with the activity of antioxidative enzymes and negatively correlated with morphological parameters. Significant positive correlation between various morphological parameters showed their interdependency. However, negative correlation was obtained between activity of antioxidative enzymes and morphological parameters. The enzymes however, showed positive correlation with each other. Based on our result we can conclude that all morphological parameters were adversely affected by the two pesticides as reflected by phytotoxicity in Allium . Their negative correlation with activity of antioxidative enzymes indicates that upregulation of antioxidative enzymes is not sufficient to overcome the toxic effect, thereby signifying the threat being caused by the regular use of these pesticides.

  3. Therapeutic Effects of Allium sativum and Allium cepa in Schistosoma mansoni experimental infection

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    Mona Mohamed Mantawy

    2011-06-01

    Full Text Available The effects of both garlic (Allium sativum and onion (Allium cepa on some biochemical parameters in Schistosoma mansoni infected mice individually and mixed either with or without the currently used drug, praziquantel (PZQ were investigated. These involved some immunological parameters, namely IgM, IgG, interleukins 2 and 6 (IL-2 and 6 and tumor necrosis factor (TNF-α, some antioxidant enzymes [catalase, superoxide dismutase (SOD and glutathione peroxidase (GPX]. In addition, parasitological and histopathological investigations were performed. No changes were observed in the normal control mice treated with dry extract of onion or garlic, individually or mixed, with or without PZQ, compared to the normal healthy control group. Infection with S. mansoni showed an increase in IgG, IgM, IL-2, IL-6, TNF-α and catalase enzyme, accompanied with a decrease in GPX and SOD antioxidant enzyme activities. Remarkable amelioration was noticed in the levels of all the measured parameters in S. mansoni infected mice after administration of the studied extracts. Moreover a significant reduction in worm burden, hepatic and intestinal eggs and oogram count was noticed which was reflected in normalization of liver architecture.

  4. Chromosome aberrations in space-exposed seeds of Allium cepa L

    International Nuclear Information System (INIS)

    Wang, S.

    1994-01-01

    Onion (Allium cepa L.) seeds c.v. Patti King were packed in sealed canisters and launched into space orbit about 296 km above earth by the space shuttle Challenger in April 1984. After more than five years exposure to space, the seeds were retrieved by the space shuttle Columbia and returned to earth in January, 1990. Somatic chromosomes at anaphase and telophase stages were analyzed and cells with normal or abnormal chromosome separations were recorded. Space-exposed and control seeds showed an average of 10.9% and 2.8% chromosome aberrations, respectively. Seeds contained in the two exterior layers of the canister had 16.5 to 18.5% chromosome aberration. The results indicated that irradiation in space would be a direct cause for chromosome aberrations in onion seeds. Analysis of seed germination rate and vigor were also determined. The average germination rate for space-exposed and control seeds were 53.3% and 19.8%, respectively. Possible reasons for the results obtained are discussed [it

  5. Biomphalaria straminea no Peru e sua suscetibilidade a cepas brasileiras de Schistosoma mansoni

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    C.A. Cuba Cuba

    1977-12-01

    Full Text Available Em Maio de 1973, um dos autores (C.A.C. coletou na localidade de Imacita, Província de Bagua, Departamento de Amazonas, vários espécimes de Biomphalaria straminea (Dunker, 1848, uma espécie que, até então, não havia sido assinalada no Peru. Descendentes destes indivíduos foram submetidos a provas de suscetibilidade às cepas BH e SJ de Schistosoma mansoni que, em condições naturais, evoluem em B. glabrata de Belo Horizonte e B. tenagophila de São José dos Campos, respectivamente. Oitenta espécimens foram expostos à cepa BH dos quais em 13 ou 16,2% a infecção evoluiu caracteristicamente até a formação de esporocistos secundários sem haver, contudo, eliminação de cercárias, mesmo no indivíduo que apresentou uma sobrevivência de 88 dias após a exposição. Não se verificou cura espontânea neste lote. Entre as 40 B. straminea expostas à cepa SJ 9 ou 22,5% infectaram-se, sendo que apenas duas eliminaram poucas cercárias aos 57 e 77 dias após a exposição, por dois dias consecutivos, tendo uma morrido e uma se curado espontaneamente. A cura espontânea do parasitismo foi notado em mais dois indivíduos, nos quais a infecção foi observada através da concha. Cortes histológicos seriados de 9 caramujos, expostos individualmente a 50 miracídios da cepa BH e fixados entre 6 e 120 horas após a exposição, mostraram esporocistos em desenvolvimento e esporocistos invadidos por amebócitos, sem formação de granulomas por parte do hospedeiro, fato assinalado em caramujos suscetíveis. A população estudada comportou-se experimentalmente de modo semelhante a outras populações de B. straminea testadas em laboratório, isto é, com baixa suscetibilidade, embora tal comportamento não afaste a possibilidade dela vir a manter o ciclo do parasita em sua área de distribuição.In May 1973 one of the Authors (C.A.C. collected specimens of Biomphalaria straminea (Dunker 1848 at imacita, Bagua Province, Amazonas, Pem. This

  6. Evaluación de la resistencia a insecticidas de una cepa de Aedes aegypti de El Salvador Assessing the insecticide resistance of an Aedes aegypti strain in El Salvador

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    Juan A. Bisset Lazcano

    2009-09-01

    Full Text Available OBJETIVOS: Evaluar el nivel de susceptibilidad a insecticidas de una cepa de Aedes aegypti procedente de El Salvador y describir los posibles mecanismos de resistencia al temefós. MÉTODOS: Se evaluó una cepa de A. aegypti procedente del municipio de Soyapango, departamento de San Salvador, El Salvador. Mediante bioensayos se determinó la susceptibilidad de las larvas al insecticida organofosforado temefós y a tres piretroides (deltametrina, lambdacialotrina y cipermetrina y de los adultos a un insecticida organofosforado (clorpirifós. Se determinó el factor de resistencia (FR50 con respecto a una cepa sensible de referencia (Rockefeller. Se estableció el mecanismo de resistencia al temefós mediante el empleo de sustancias sinergistas, ensayos bioquímicos de actividad enzimática y zimogramas en gel de poliacrilamida. RESULTADOS: Las larvas de la cepa estudiada mostraron una alta resistencia al temefós (FR50 = 24,16. De las enzimas analizadas, se encontró que solo la esterasa A4 estaba vinculada al mecanismo de resistencia al temefós. Los mosquitos adultos resultaron susceptibles a la lambdacialotrina y al clorpirifós y su resistencia a la deltametrina y la cipermetrina quedó en la categoría de verificación. CONCLUSIONES: La resistencia al temefós podría reducir la eficacia del control químico del mosquito A. aegypti en la zona estudiada de El Salvador. Los insecticidas clorpirifós, lambdacialotrina y cipermetrina son buenos candidatos alternativos a utilizar en las nuevas intervenciones de control de este vector.OBJECTIVES: To assess the level of insecticide susceptibility of a certain Aedes aegypti strain found in El Salvador and to explain the mechanisms for its resistance to temephos. METHODS: An A. aegypti strain from the municipality of Soyapango, Department of San Salvador, El Salvador, was studied. Bioassays were used to determine the susceptibility of the larvae to the organophosphate insecticide temephos and to

  7. Studies on the biosynthesis of lignins and their production in plant cell cultures. [Forsythia intermedia; Podophyllum hexandrum; Podophyllum peltatum

    Energy Technology Data Exchange (ETDEWEB)

    Abdul-Rahman, M.M.

    1988-01-01

    Phytochemical analysis of Forsythia intermedia tissues has demonstrated the presence of lignins of the dibenzylbutyrolactone and dioxabicyclo(3,3,0)octane classes, together with their O-glucosides. Lignin distribution in different parts of the part, and variation with the season has been recorded. Cell suspension cultures from F. intermedia were developed. These also synthesized lignins, though not the full range as found in the fresh tissues. Culture lines synthesizing either matairesinol 4{prime}-O-glucoside and major lignin components were established. These cultures rapidly metabolized exogenous lignins without producing detectable lignin transformation products. The seasonal variation in aryltetralin lactone levels in young plants Podophyllum hexandrum and P. peltatum has been investigated. Cell cultures of the plants were established, but no lignins were detectable in them. However, a protocol for micropropagation via embryoid production was obtained. Feeding experiments in P. hexandrum plants showed that matairesinol was a precursor of both podophyllotoxin and 4{prime}-demethylpodophyllotoxin, thus indicating it to be a common precursor of the trimethoxy and hydroxydimethoxy series of lignins in this plant. Precursor feeding experiments with {sup 3}H/{sup 14}C-labeled coniferyl alcohol showed an unexpected increase in {sup 3}H/{sup 14}C ratio when incorporated into both Forsythia and Podophyllum lignins.

  8. Periodontitis in cardiovascular disease patients with or without Marfan syndrome--a possible role of Prevotella intermedia.

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    Jun-ichi Suzuki

    Full Text Available BACKGROUND: Although periodontitis is a risk factor for cardiovascular disease (CVD, the influence of periodontitis on Marfan syndrome (MFS with CVD is unclear. The aim of this study was to assess the relationship between periodontal bacterial burden and MSF with CVD. METHODS AND RESULTS: The subjects were patients with MFS with CVD (n = 47; age and gender matched non-MFS CVD patients (n = 48 were employed as controls. Full-mouth clinical measurements, including number of teeth, probing of pocket depth (PD, bleeding on probing (BOP and community periodontal index (CPI were recorded. We also evaluated the existence of three periodontal pathogens, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Prevotella intermedia using polymerase chain reaction assays. Serum antibody titers against the pathogens were also measured. We revealed that MFS with CVD patients had periodontitis more frequently than the age and gender matched non-MFS CVD control subjects. MFS with CVD patients had significantly severer periodontitis, fewer remaining teeth and deeper PD compared to the non-MFS CVD controls. Furthermore, the serum antibody titer level against Prevotella intermedia was significantly lower in MFS plus CVD patients compared to the non-MFS CVD patients. CONCLUSION: Periodontitis may influence the pathophysiology of cardiovascular complications in MFS patients. A specific periodontal pathogen might be a crucial therapeutic target to prevent CVD development.

  9. Liver transplantation from a deceased donor with β-thalassemia intermedia is not contraindicated: A case report.

    Science.gov (United States)

    Gumus, Ersin; Abbasoglu, Osman; Tanyel, Cahit; Gumruk, Fatma; Ozen, Hasan; Yuce, Aysel

    2017-05-01

    The use of extended criteria donors who might have previously been deemed unsuitable is an option to increase the organ supply for transplantation. This report presents a pediatric case of a successful liver transplantation from a donor with β-thalassemia intermedia. A patient, 6-year-old female, with a diagnosis of cryptogenic liver cirrhosis underwent deceased donor liver transplantation from a thalassemic donor. Extreme hyperferritinemia was detected shortly after transplantation. The most probable cause of hyperferritinemia was iron overload secondary to transplantation of a hemosiderotic liver. Hepatocellular injury due to acute graft rejection might have contributed to elevated ferritin levels by causing release of stored iron from the hemosiderotic liver graft. Iron chelation and phlebotomy therapies were started simultaneously in the early postoperative period to avoid iron-related organ toxicity and transplant failure. Follow-up with monthly phlebotomies after discharge yielded a favorable outcome with normal transplant functions. Thalassemia intermedia patients can be candidates of liver donors to decrease pretransplant waitlist mortality. After transplantation of a hemosiderotic liver, it is important to monitor the recipient in terms of iron overload and toxicity. Early attempts to lower iron burden including chelation therapy and/or phlebotomy should be considered to avoid organ toxicity and transplant failure. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  10. A Preliminary Study of the Effects of pH upon Fluorescence in Suspensions of Prevotella intermedia.

    Science.gov (United States)

    Hope, Christopher K; Billingsley, Karen; de Josselin de Jong, Elbert; Higham, Susan M

    2016-01-01

    The quantification of fluorescence in dental plaque is currently being developed as a diagnostic tool to help inform and improve oral health. The oral anaerobe Prevotella intermedia exhibits red fluorescence due to the accumulation of porphyrins. pH affects the fluorescence of abiotic preparations of porphyrins caused by changes in speciation between monomers, higher aggregates and dimers, but this phenomenon has not been demonstrated in bacteria. Fluorescence spectra were obtained from suspensions of P. intermedia that were adjusted to pHs commensurate with the range found within dental plaque. Two fluorescent motifs were identified; 410 nm excitation / 634 nm emission (peak A) and 398 nm excitation / 622 nm emission (peak B). A transition in the fluorescence spectra was observed from peak A to peak B with increasing pH which was also evident as culture age increased from 24 hours to 96 hours. In addition to these 'blue-shifts', the intensity of peak A increased with pH whilst decreasing with culture age from 24 to 96 hours. A bacterium's relationship with the local physiochemical environment at the time of image capture may therefore affect the quantification of dental plaque fluorescence.

  11. Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom

    International Nuclear Information System (INIS)

    Ullah, Anwar; Giuseppe, Priscila Oliveira de; Murakami, Mario Tyago; Trevisan-Silva, Dilza; Wille, Ana Carolina Martins; Chaves-Moreira, Daniele; Gremski, Luiza Helena; Silveira, Rafael Bertoni da; Sennf-Ribeiro, Andrea; Chaim, Olga Meiri; Veiga, Silvio Sanches; Arni, Raghuvir Krishnaswamy

    2011-01-01

    Wild-type and H12A-mutant class II phospholipase D from L. intermedia venom were crystallized; the crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively. Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12 1 1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 Å, β = 105.9°. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively

  12. Differential Midgut Attachment of Leishmania (Viannia braziliensis in the Sand Flies Lutzomyia (Nyssomyia whitmani and Lutzomyia (Nyssomyia intermedia

    Directory of Open Access Journals (Sweden)

    Rodrigo P. Soares

    2010-01-01

    Full Text Available The interaction between Leishmania and sand flies has been demonstrated in many Old and New World species. Besides the morphological differentiation from procyclic to infective metacyclic promastigotes, the parasite undergoes biochemical transformations in its major surface lipophosphoglycan (LPG. An upregulation of β-glucose residues was previously shown in the LPG repeat units from procyclic to metacyclic phase in Leishmania (Viannia braziliensis, which has not been reported in any Leishmania species. LPG has been implicated as an adhesion molecule that mediates the interaction with the midgut epithelium of the sand fly in the Subgenus Leishmania. These adaptations were explored for the first time in a species from the Subgenus Viannia, L. (V. braziliensis with its natural vectors Lutzomyia (Nyssomyia intermedia and Lutzomyia (Nyssomyia whitmani. Using two in vitro binding techniques, phosphoglycans (PGs derived from procyclic and metacyclic parasites were able to bind to the insect midgut and inhibit L. braziliensis attachment. Interestingly, L. braziliensis procyclic parasite attachment was ∼11-fold greater in the midgut of L. whitmani than in L. intermedia. The epidemiological relevance of L. whitmani as a vector of American Cutaneous Leishmaniasis (ACL in Brazil is discussed.

  13. Detection of Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia in primary endodontic infections in a Chinese population.

    Science.gov (United States)

    Cao, H; Qi, Z; Jiang, H; Zhao, J; Liu, Z; Tang, Z

    2012-08-01

    To assess the prevalence of three black-pigmented bacterial species (Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia) using microarray technology in root canals of teeth associated with primary endodontic infections in a Chinese population. Microbial samples were taken from root canals of 80 teeth with pulp necrosis and primary endodontic infections in a Chinese population. DNA extracted from the samples was amplified by PCR with universal bacterial primers based on 16S rRNA gene sequences, and the products hybridized with the microarrays in which the specific oligonucleotide probes were added. The results of hybridization were screened by a confocal laser scanner. Pearson chi-square test and the two-sided Fisher exact test were used to analyse whether a significant association existed between the species and symptoms as well as in co-existence of two target organisms by a statistical software package (SAS 8.02). The 16S rRNA gene microarray detected at least one of the three test species in 76% of the study teeth. P. endodontalis, P. gingivalis and P. intermedia were found in 50%, 33% and 45%, respectively. A significant association was found in the presence of the pair P. endodontalis / P. gingivalis (P endodontalis (P endodontalis and P. gingivalis was also associated with the presence of a sinus tract (Pendodontalis were associated with the presence of sinus tract and abscess formation. © 2012 International Endodontic Journal.

  14. Resistencia antimicrobiana de cepas de Bartonella bacilliformis procedentes de regiones endémicas de la Enfermedad de Carrión en EL Perú

    Directory of Open Access Journals (Sweden)

    Giovanna Mendoza-Mujica

    Full Text Available Objetivos. Evaluar la susceptibilidad antimicrobiana in vitro a cloranfenicol (CHL y ciprofloxacino (CIP de cepas de Bartonella bacilliformis procedentes de áreas endémicas de la enfermedad de Carrión (EC en el Perú, mediante tres métodos de laboratorio. Materiales y métodos. Se evaluó la susceptibilidad antimicrobiana a CHL y CIP de 100 cepas de Bartonella bacilliformis, los aislamientos procedieron de pacientes de los departamentos de Ancash, Cusco, Cajamarca, Lima y La Libertad; las cepas se evaluaron mediante: disco difusión, E-Test y dilución en agar. Resultados. El 26% de las cepas de Bartonella bacilliformis evaluadas, presentaron resistencia a CIP y 1% a CHL. Se obtuvieron patrones similares de sensibilidad/resistencia antimicrobiana en los tres métodos utilizados. Conclusiones. Las cepas de Bartonella bacilliformis circulantes en el Perú, presentan elevados niveles de resistencia in vitro a CIP, por lo que se recomienda ampliar la investigación sobre la utilización del fármaco en los esquemas de tratamiento de la EC. Los métodos de E-test y disco difusión resultaron más convenientes para la evaluación de la susceptibilidad antimicrobiana in vitro del microorganismo

  15. ESTABILIDAD DE ALMACENAMIENTO DE ENSILADOS BIOLÓGICOS A PARTIR DE RESIDUOS DE PESCADO INOCULADOS CON BACTERIAS ÁCIDO -LÁCTICAS

    Directory of Open Access Journals (Sweden)

    MS Holguín

    2009-01-01

    Full Text Available Se elaboraron cuatro muestras por triplicado de ensilados biológicos para alimentación animal a partir de residuos de pescado, utilizando melaza como fuente de carbohidratos para el crecimiento de cuatro cepas de bacterias ácido-lácticas (BAL aisladas de los mismos, sometidos a un tiempo de incubación de 72 horas y temperatura de 35 °C (±2 °C para acidificar el producto como método de conservación. A continuación los ensilados se almacenaron durante 180 días a temperatura ambiente para evaluar la estabilidad en anaquel, por medio de análisis químicos, composición química proximal, aminograma, recuentos microbiológicos y algunos de tipo organoléptico del producto terminado. Las cepas fueron eficientes en el proceso de fermentación, causando inhibición del crecimiento de microorganismos indeseables y aportando características organolépticas agradables. El ensilado elaborado con la cepa C14 provocó el descenso del pH en menos de 72 horas de incubación. Ninguno de los productos sufrió deterioro evidente durante el almacenamiento; presentaron porcentajes aceptables de proteína, grasa, cenizas, carbohidratos y aminoácidos, que hacen del producto una fuente utilizable en formulaciones de alimentos para animales.

  16. Intermedin A, a New Labdane Diterpene Isolated from Alpinia intermedia, Prolonged the Survival Time of P-388D1 Tumor-Bearing CDF1 Mice.

    Science.gov (United States)

    Chen, Lih-Geeng; Su, Pei-Jung; Tsai, Po-Wei; Yang, Ling-Ling; Wang, Ching-Chiung

    2017-01-01

    Eight ethanolic extracts of indigenous Taiwanese plants of the genus Alpinia were tested for tumor cytotoxicity against AGS, Hep G2, HeLa, KB, and HL-60 cells. Among the 50 % and 95 % EtOH extracts of eight Alpinia species, the cytotoxic effects of Alpinia intermedia leaves were the strongest. When the leaf extract of A. intermedia was partitioned using n -hexane and aqueous solvents, the n -hexane layer showed a greater cytotoxic effect and could prolong the survival time of P-388D 1 tumor-bearing CDF1 mice. Two new labdane diterpene derivatives, intermedin A ( 1 ) and intermedin B ( 2 ), and coronarin E ( 3 ) were isolated from the n -hexane layer of A. intermedia . Intermedin A induced apoptosis in HL-60 cells at 30 µg/mL and significantly prolonged the survival time of P-388D 1 tumor-bearing CDF 1 mice by 48.7 % at 20 mg/kg of body weight. We suggest that intermedin A is a major compound of A. intermedia and has a cytotoxic effect on HL-60 and P-388D 1 cells. Georg Thieme Verlag KG Stuttgart · New York.

  17. Cómo se negocia una posición de sujeto intermedia: muchachos entre once y catorce años, masculinidades y educación escolar

    Directory of Open Access Journals (Sweden)

    Ann Phoenix

    2002-04-01

    Full Text Available Este ensayo pone a discusión la ciencia (generalizada en las escuelas de Londres según la cual existe una oposición entre la masculinidad y la realización de tareas escolares, que tiene fuertes efectos en la subjetividad de lo masculino del joven. Así considera las formas en que éstos asisten a las escuelas de Londres y negocian lo que ellos consideran las contradicciones que involucra el tratar de reconciliar el hacer las tareas escolares con ser aceptado por los otros en su condición masculina. El documento se centra particularmente en la forma como los muchachos negocian una posición de sujeto intermedia, en un intento de asegurarse que trabajaron lo suficiente para no reprobar, pero no tanto como para llamar la atención de los otros, por no ser “propiamente masculinos”. El texto también afirma que estas contradicciones tienen una carga emocional, de modo que los jóvenes se esfuerzan en negociar lo que ellos consideran una posición intermedia, pues esta es la posición de sujeto que les resulta menos problemática ocupar.This paper discusses how the construction (pervasive in London schools that masculinities and doing schoolwork are antithetical to each other has an impact on boys’ subject positions. It considers the ways in which boys attending schools in London negotiate what they see as the contradictions involved in attempting to reconcile doing schoolwork with being accepted by other boys as masculine.In particular, it focuses on how they negotiate a middle subject position, attempting to ensure that they did sufficient work not to fail at school, but not so much that they came to other boys’ attention as not ‘properly masculine’. The paper argues that these contradictions are emotionally charged, so that boys put effort into negotiating what they see as a middle position because this is the least problematic subject position for them to take up.

  18. NCX 4040, a nitric oxide-donating aspirin derivative, inhibits Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

    Science.gov (United States)

    Choi, Eun-Young; Choe, So-Hui; Hyeon, Jin-Yi; Park, Hae Ryoun; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

    2015-12-05

    In this study, the effects and underlying mechanisms of NCX 4040, a nitric oxide (NO)-donating aspirin derivative, on the production of proinflammatory mediators were examined using murine macrophages exposed to lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in the etiology of periodontal disease. NCX 4040 significantly reduced P. intermedia LPS-induced production of inducible NO synthase (iNOS)-derived NO, IL-1β and IL-6 as well as their mRNA expression in RAW264.7 cells. Notably, NCX 4040 was much more effective than the parental compound aspirin in reducing LPS-induced production of inflammatory mediators. NCX 4040 induced the expression of heme oxygenase-1 (HO-1) in cells treated with P. intermedia LPS, and the suppressive effect of NCX 4040 on LPS-induced NO production was significantly reversed by SnPP, a competitive HO-1 inhibitor. NCX 4040 did not influence LPS-induced phosphorylation of JNK and p38. IκB-α degradation as well as nuclear translocation and DNA-binding activities of NF-κB p65 and p50 subunits induced by P. intermedia LPS were significantly reduced by NCX 4040. Besides, LPS-induced phosphorylation of STAT1 and STAT3 was significantly down-regulated by NCX 4040. Further, NCX 4040 elevated the SOCS1 mRNA in cells stimulated with LPS. This study indicates that NCX 4040 inhibits P. intermedia LPS-induced production of NO, IL-1β and IL-6 in murine macrophages through anti-inflammatory HO-1 induction and suppression of NF-κB, STAT1 and STAT3 activation, which is associated with the activation of SOCS1 signaling. NCX 4040 could potentially be a promising tool in the treatment of periodontal disease, although further studies are required to verify this. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. The effect of oleander glycosides on the germination of pollen grains and the mitosis of the generative nucleus in Tradescantia bracteata Small and Allium cepa L.

    Directory of Open Access Journals (Sweden)

    J. A. Tarkowska

    2015-01-01

    Full Text Available The effect of water solution of a mixture of glycosides from oleander (Nerium oleander L. on the germination of pollen grains and on the mitosis of the generative nucleus in Tradescantia bracteata Small and Allium cepa L. has been studied. An inhibition of the germination and of the growth of pollen tubes was observed, proportionally to the concentration of glycosides. The pollen grains of A. cepa are more sensitive. The disturbances in mitosis lead to the formation of two or more uneven-sized doughter nuclei, or to the formation of restitution nuclei. These anomalies are more numerous in T. bracteata. From these results d t appears that pollen grains of A. cepa are characterized by a generally high physiological sensitivity and a small mitotic sensitivity, wheras for T. bracteata the opposite is true.

  20. The onion (Allium cepa L. R2R3-MYB gene MYB1 regulates anthocyanin biosynthesis

    Directory of Open Access Journals (Sweden)

    Kathy Schwinn

    2016-12-01

    Full Text Available Bulb colour is an important consumer trait for onion (Allium cepa L., Allioideae, Asparagales. The bulbs accumulate a range of flavonoid compounds, including anthocyanins (red, flavonols (pale yellow and chalcones (bright yellow. Flavonoid regulation is poorly characterised in onion and in other plants belonging to the Asparagales, despite being a major plant order containing many important crop and ornamental species. R2R3-MYB transcription factors associated with the regulation of distinct branches of the flavonoid pathway were isolated from onion. These belonged to sub-groups (SGs that commonly activate anthocyanin (SG6, MYB1 or flavonol (SG7, MYB29 production, or repress phenylpropanoid/flavonoid synthesis (SG4, MYB4, MYB5. MYB1 was demonstrated to be a positive regulator of anthocyanin biosynthesis by the induction of anthocyanin production in onion tissue when transiently overexpressd and by reduction of pigmentation when transiently repressed via RNAi. Furthermore, ectopic red pigmentation was observed in garlic (A. sativum L. plants stably transformed with a construct for co-overexpression of MYB1 and a bHLH partner. MYB1 also was able to complement the acyanic petal phenotype of a defined R2R3-MYB anthocyanin mutant in Antirrhinum majus of the asterid clade of eudicots. The availability of sequence information for flavonoid-related MYBs from onion enabled phylogenetic groupings to be determined across monocotyledonous and dicotyledonous species, including the identification of characteristic amino acid motifs. This analysis suggests that divergent evolution of the R2R3-MYB family has occurred between Poaceae/Orchidaceae and Allioideae species. The DNA sequences identified will be valuable for future analysis of classical flavonoid genetic loci in Allium crops and will assist the breeding of these important crop species.

  1. The Onion (Allium cepa L.) R2R3-MYB Gene MYB1 Regulates Anthocyanin Biosynthesis

    Science.gov (United States)

    Schwinn, Kathy E.; Ngo, Hanh; Kenel, Fernand; Brummell, David A.; Albert, Nick W.; McCallum, John A.; Pither-Joyce, Meeghan; Crowhurst, Ross N.; Eady, Colin; Davies, Kevin M.

    2016-01-01

    Bulb color is an important consumer trait for onion (Allium cepa L., Allioideae, Asparagales). The bulbs accumulate a range of flavonoid compounds, including anthocyanins (red), flavonols (pale yellow), and chalcones (bright yellow). Flavonoid regulation is poorly characterized in onion and in other plants belonging to the Asparagales, despite being a major plant order containing many important crop and ornamental species. R2R3-MYB transcription factors associated with the regulation of distinct branches of the flavonoid pathway were isolated from onion. These belonged to sub-groups (SGs) that commonly activate anthocyanin (SG6, MYB1) or flavonol (SG7, MYB29) production, or repress phenylpropanoid/flavonoid synthesis (SG4, MYB4, MYB5). MYB1 was demonstrated to be a positive regulator of anthocyanin biosynthesis by the induction of anthocyanin production in onion tissue when transiently overexpressed and by reduction of pigmentation when transiently repressed via RNAi. Furthermore, ectopic red pigmentation was observed in garlic (Allium sativum L.) plants stably transformed with a construct for co-overexpression of MYB1 and a bHLH partner. MYB1 also was able to complement the acyanic petal phenotype of a defined R2R3-MYB anthocyanin mutant in Antirrhinum majus of the asterid clade of eudicots. The availability of sequence information for flavonoid-related MYBs from onion enabled phylogenetic groupings to be determined across monocotyledonous and dicotyledonous species, including the identification of characteristic amino acid motifs. This analysis suggests that divergent evolution of the R2R3-MYB family has occurred between Poaceae/Orchidaceae and Allioideae species. The DNA sequences identified will be valuable for future analysis of classical flavonoid genetic loci in Allium crops and will assist the breeding of these important crop species. PMID:28018399

  2. Transcriptome Analysis of Sucrose Metabolism during Bulb Swelling and Development in Onion (Allium cepa L.

    Directory of Open Access Journals (Sweden)

    Yi Liang

    2016-09-01

    Full Text Available Allium cepa L. is a widely cultivated and economically significant vegetable crop worldwide, with beneficial dietary and health-related properties, but its sucrose metabolism is still poorly understood. To analyze sucrose metabolism during bulb swelling, and the development of sweet taste in onion, a global transcriptome profile of onion bulbs was undertaken at three different developmental stages, using RNA-seq. A total of 79,376 unigenes, with a mean length of 678 bp, was obtained. In total, 7% of annotated Clusters of Orthologous Groups (COG were involved in carbohydrate transport and metabolism. In the Kyoto Encyclopedia of Genes and Genomes (KEGG database, starch and sucrose metabolism (147, 2.40% constituted the primary metabolism pathway in the integrated library. The expression of sucrose transporter genes was greatest during the early-swelling stage, suggesting that sucrose transporters participated in sucrose metabolism mainly at an early stage of bulb development. A gene-expression analysis of the key enzymes of sucrose metabolism suggested that sucrose synthase, cell wall invertase and invertase were all likely to participate in the hydrolysis of sucrose, generating glucose and fructose. In addition, trehalose was hydrolyzed to two molecules of glucose by trehalase. From 15 to 40 days after swelling (DAS, both the glucose and fructose contents of bulbs increased, whereas the sucrose content decreased. The growth rate between 15 and 30 DAS was slower than that between 30 and 40 DAS, suggesting that the latter was a period of rapid expansion. The dataset generated by our transcriptome profiling will provide valuable information for further research.

  3. Hypersensitivity of Allium cepa seedling roots to X-rays for production of micronuclei

    International Nuclear Information System (INIS)

    Hori, Takayoshi; Hanmoto, Hidehiro; Fujishige, Ikuko; Inoue, Toshihiro; Taniguchi, Kenji; Itoh, Tetsuo; Fujikawa, Kazuo; Yonezawa, Yoshihiko.

    1995-01-01

    Seedling roots of onion (Allium cepa) were irradiated with various doses of X-rays. Following irradiation, the roots were incubated at 23-25degC for 24 hr, i.e., approximate time for one mitotic cycle, and then fixed, stained and macerated in a 7:3 mixture of acetic dahlia (prepared by dissolving a 0.5 g sample of dahlia violet into 100 ml of 30% acetate) and 1 N HCl for 10-15 min. Terminal 1-2 mm of the root tips were squashed on slides, one root each, and microscopically inspected for the presence of more than one nucleus in the cells. The additional nuclei, which were smaller than the normal, were scored as micronuclei. The frequency of micronuclei increased with dose over the control level (∼0.2x10 -3 ) to a high level of 140x10 -3 at 1 Gy. The frequency recorded at 1 Gy was about two fold higher as compared with the frequency reported by Evans et al. (1959) for micronuclei induced by γ-rays at a comparable dose in the root tip cells of Vica faba seedlings, probably reflecting relatively higher DNA content per cell in Allium somatic cells. We thus may conclude that root-tip meristematic cells of Allium seedlings are hypersensitive to the induction of micronuclei by X-rays. The Allium micronucleus assay may be useful as a system not only for quantitating chromosome damage by low-level radiation but also for detecting environmental mutagens. (author)

  4. Identification of pathogenicity-related genes in Fusarium oxysporum f. sp. cepae.

    Science.gov (United States)

    Taylor, Andrew; Vágány, Viktória; Jackson, Alison C; Harrison, Richard J; Rainoni, Alessandro; Clarkson, John P

    2016-09-01

    Pathogenic isolates of Fusarium oxysporum, distinguished as formae speciales (f. spp.) on the basis of their host specificity, cause crown rots, root rots and vascular wilts on many important crops worldwide. Fusarium oxysporum f. sp. cepae (FOC) is particularly problematic to onion growers worldwide and is increasing in prevalence in the UK. We characterized 31 F. oxysporum isolates collected from UK onions using pathogenicity tests, sequencing of housekeeping genes and identification of effectors. In onion seedling and bulb tests, 21 isolates were pathogenic and 10 were non-pathogenic. The molecular characterization of these isolates, and 21 additional isolates comprising other f. spp. and different Fusarium species, was carried out by sequencing three housekeeping genes. A concatenated tree separated the F. oxysporum isolates into six clades, but did not distinguish between pathogenic and non-pathogenic isolates. Ten putative effectors were identified within FOC, including seven Secreted In Xylem (SIX) genes first reported in F. oxysporum f. sp. lycopersici. Two highly homologous proteins with signal peptides and RxLR motifs (CRX1/CRX2) and a gene with no previously characterized domains (C5) were also identified. The presence/absence of nine of these genes was strongly related to pathogenicity against onion and all were shown to be expressed in planta. Different SIX gene complements were identified in other f. spp., but none were identified in three other Fusarium species from onion. Although the FOC SIX genes had a high level of homology with other f. spp., there were clear differences in sequences which were unique to FOC, whereas CRX1 and C5 genes appear to be largely FOC specific. © 2015 The Authors Molecular Plant Pathology Published by British Society for Plant Pathology and John Wiley & Sons Ltd.

  5. Identification of pathogenicity‐related genes in Fusarium oxysporum f. sp. cepae

    Science.gov (United States)

    Vágány, Viktória; Jackson, Alison C.; Harrison, Richard J.; Rainoni, Alessandro; Clarkson, John P.

    2016-01-01

    Summary Pathogenic isolates of Fusarium oxysporum, distinguished as formae speciales (f. spp.) on the basis of their host specificity, cause crown rots, root rots and vascular wilts on many important crops worldwide. Fusarium oxysporum f. sp. cepae (FOC) is particularly problematic to onion growers worldwide and is increasing in prevalence in the UK. We characterized 31 F. oxysporum isolates collected from UK onions using pathogenicity tests, sequencing of housekeeping genes and identification of effectors. In onion seedling and bulb tests, 21 isolates were pathogenic and 10 were non‐pathogenic. The molecular characterization of these isolates, and 21 additional isolates comprising other f. spp. and different Fusarium species, was carried out by sequencing three housekeeping genes. A concatenated tree separated the F. oxysporum isolates into six clades, but did not distinguish between pathogenic and non‐pathogenic isolates. Ten putative effectors were identified within FOC, including seven Secreted In Xylem (SIX) genes first reported in F. oxysporum f. sp. lycopersici. Two highly homologous proteins with signal peptides and RxLR motifs (CRX1/CRX2) and a gene with no previously characterized domains (C5) were also identified. The presence/absence of nine of these genes was strongly related to pathogenicity against onion and all were shown to be expressed in planta. Different SIX gene complements were identified in other f. spp., but none were identified in three other Fusarium species from onion. Although the FOC SIX genes had a high level of homology with other f. spp., there were clear differences in sequences which were unique to FOC, whereas CRX1 and C5 genes appear to be largely FOC specific. PMID:26609905

  6. The Onion (Allium cepa L.) R2R3-MYB Gene MYB1 Regulates Anthocyanin Biosynthesis.

    Science.gov (United States)

    Schwinn, Kathy E; Ngo, Hanh; Kenel, Fernand; Brummell, David A; Albert, Nick W; McCallum, John A; Pither-Joyce, Meeghan; Crowhurst, Ross N; Eady, Colin; Davies, Kevin M

    2016-01-01

    Bulb color is an important consumer trait for onion ( Allium cepa L., Allioideae, Asparagales). The bulbs accumulate a range of flavonoid compounds, including anthocyanins (red), flavonols (pale yellow), and chalcones (bright yellow). Flavonoid regulation is poorly characterized in onion and in other plants belonging to the Asparagales, despite being a major plant order containing many important crop and ornamental species. R2R3-MYB transcription factors associated with the regulation of distinct branches of the flavonoid pathway were isolated from onion. These belonged to sub-groups (SGs) that commonly activate anthocyanin (SG6, MYB1) or flavonol (SG7, MYB29) production, or repress phenylpropanoid/flavonoid synthesis (SG4, MYB4, MYB5). MYB1 was demonstrated to be a positive regulator of anthocyanin biosynthesis by the induction of anthocyanin production in onion tissue when transiently overexpressed and by reduction of pigmentation when transiently repressed via RNAi. Furthermore, ectopic red pigmentation was observed in garlic ( Allium sativum L.) plants stably transformed with a construct for co-overexpression of MYB1 and a bHLH partner. MYB1 also was able to complement the acyanic petal phenotype of a defined R2R3-MYB anthocyanin mutant in Antirrhinum maju s of the asterid clade of eudicots. The availability of sequence information for flavonoid-related MYBs from onion enabled phylogenetic groupings to be determined across monocotyledonous and dicotyledonous species, including the identification of characteristic amino acid motifs. This analysis suggests that divergent evolution of the R2R3-MYB family has occurred between Poaceae/Orchidaceae and Allioideae species. The DNA sequences identified will be valuable for future analysis of classical flavonoid genetic loci in Allium crops and will assist the breeding of these important crop species.

  7. Strip Tillage and Early-Season Broadleaf Weed Control in Seeded Onion (Allium cepa

    Directory of Open Access Journals (Sweden)

    Sarah Gegner-Kazmierczak

    2016-03-01

    Full Text Available Field experiments were conducted in 2007 and 2008 near Oakes, North Dakota (ND, USA, to evaluate if strip tillage could be incorporated into a production system of seeded onion (Allium cepa to eliminate the standard use of a barley (Hordeum vulgare companion crop with conventional, full width tillage, yet support common early-season weed control programs. A split-factor design was used with tillage (conventional and strip tillage as the main plot and herbicide treatments (bromoxynil, DCPA, oxyfluorfen, and pendimethalin as sub-plots. Neither tillage nor herbicide treatments affected onion stand counts. Common lambsquarters (Chenopodium album densities were lower in strip tillage compared to conventional tillage up to three weeks after the post-emergence applied herbicides. In general, micro-rate post-emergence herbicide treatments provided greater early-season broadleaf weed control than pre-emergence herbicide treatments. Onion yield and grade did not differ among herbicide treatments because the mid-season herbicide application provided sufficient control/suppression of the early-season weed escapes that these initial weed escapes did not impact onion yield or bulb diameter. In 2007, onion in the strip tillage treatment were larger in diameter resulting in greater total and marketable yields compared to conventional tillage. Marketable onion yield was 82.1 Mg ha−1 in strip tillage and 64.9 Mg ha−1 in conventional tillage. Results indicate that strip tillage use in direct-seeded onion production was beneficial, especially when growing conditions were conducive to higher yields and that the use of strip tillage in onion may provide an alternative to using a companion crop as it did not interfere with either early-season weed management system.

  8. Genotoxic evaluation of infusions of Urera baccifera leaves and roots in Allium cepa cells

    Directory of Open Access Journals (Sweden)

    Amanda L. Gindri

    2015-04-01

    Full Text Available Context: The aqueous extracts of Urera baccifera Wedd. leaves and roots are used to inflammatory and infectious diseases in Brazilian folk medicine. Oxalic acid, a substance co-related with toxicity and stinging, was already quantified in this plant. Aims: To evaluate the action of leaves and roots infusions (1, 30, 75 g/L and the oxalic acid standard on mitosis as indicative of presumably antimitotic and genotoxic actions, using the Allium cepa test. Methods: Oxalic acid was quantified in the roots and leaves infusions by High-performance liquid chromatography (HPLC-DAD, with the mobile phase of 25 mM phosphate buffer (pH 2.5: acetonitrile at 95:5 (v/v. To the genotoxicity test, onion bulbs were used. After the rootlets germination, each bulb was submitted for 24 h of the individual treatments. Were analyzed 1000 cells per bulb, in a total of 5000 cells per treatment. Results: Results showed that all concentrations of roots infusions induced chromosomes abnormalities, except for the highest, that caused a substantial inhibition in the mitosis, precluding to be observed abnormalities. In the leaves infusions, only the two higher concentrations caused the highest values of damage in the cellular cycle. The oxalic acid also caused abnormalities in the mitosis, and may be considered responsible by part of the genotoxic action of U. baccifera. Conclusions: Oxalic acid can be responsible by part of the chromosomal abnormalities caused by U. baccifera, although, there must have more metabolites that evoke the same effect promoting the genotoxic effect of this nettle.

  9. Comparison of the ability of fifteen onion (Allium cepa L. cultivars to accumulate nitrates

    Directory of Open Access Journals (Sweden)

    Renata Wojciechowska

    2014-04-01

    Full Text Available The aim of a two-year study was to characterise selected Allium cepa L. genotypes with regard to their ability to accumulate nitrates in bulbs as well as to search for a possible relation between NO3- concentration and dry matter content. Fifteen cultivars of edible onion, mostly of long-day genotype with different growing periods, bulb size and skin colour, were taken for the experiment. Seeds of particular cultivars were obtained from the following seed companies: Spójnia Nochowo (‘Labrador’, ‘Takstar F1’,‘Tęcza’, ‘Warna’, ‘Zorza’, Polan (‘Polanowska’, ‘Topolska’, PlantiCo Gołębiew (‘Alibaba’, ‘Efekt’, ‘Kristine’, ‘Niagara F1’,and PlantiCo Zielonki (‘Bila’, ‘Irka’, ‘Wenta’, ‘Zeta’. Plants produced from seedlings were grown in the experimental field of the University of Agriculture in Kraków. After crop harvesting and additional drying, nitrate and dry matter content in bulbs of all cultivars were measured. The following cultivars: ‘Efekt’, ‘Labrador’ and red-skinned ‘Wenta’, were characterized by the lowest ability to accumulate NO3- in bulbs. The highest nitrate content was noted in bulbs of ‘Takstar F1’ (a very early-season cultivar, followed by ‘Bila’ and ‘Tęcza’. A weak, yet statistically significant negative correlation between nitrate and dry matter content was observed. The highest dry matter content was determined in bulbs of white-skinned ‘Alibaba’, while the lowest – in brown-skinned ‘Labrador’.

  10. Alternative substrata for xaxim on culture of Cattleya intermedia X Hadrolaelia purpurata (Orchidaceae / Substratos alternativos ao xaxim no cultivo do híbrido Cattleya intermedia X Hadrolaelia purpurata (Orchidaceae

    Directory of Open Access Journals (Sweden)

    Maria Aparecida da Fonseca Sorace

    2009-12-01

    Full Text Available The present work had the purpose of evaluating the efficiency of different substrata by vegetal origin, to grow species of orchids (Orchidaceae. Seedlings of Cattleya intermedia X Hadrolaelia purpurata, obtained by in vitro culture, and acclimated, with medium height of 5 cm, had the following substrata: 1- Defibered xaxim (control; 2- Carbonized rice husk; 3- Coconut fiber; 4- Husk pínus; 5- Carbonized rice husk + Coconut fiber (1:1; 6- Carbonized rice husk + Husk pínus (1:1; 7- Coconut fiber + Husk pinus (1:1; 8- Husk pínus + Carbonized rice husk + Coconut fiber (1:1:1. A randomized design with eight treatments and ten replications. Results were submited to Variance Analysis and Tukey Test at 5 %. Growing parameters evaluated after 17 months were: root length, roots number, height of above ground, buds number, leaves number, leaf length, leaf width, total fresh mass and root volume. Determinated substrata parameters were: pH, eletric conductivity and density. All studied substratum can be recommended to substitute xaxim but, coconut fiber was least efficient to develop roots and aerial parts of plants. Better results were showed by substratum only with carbonized rice husk + husk pine on culture of Cattleya intermedia X Hadrolaelia purpurata.O presente trabalho teve como objetivo avaliar a eficiência de diferentes substratos de origem vegetal no cultivo de espécies de orquídeas. Plântulas de Cattleya intermedia X Hadrolaelia purpurata, provenientes de cultivo in vitro, aclimatizadas e com altura média de 5 cm, foram plantadas nos substratos: 1-Xaxim desfibrado (testemunha; 2-Casca de arroz carbonizada; 3-Fibra de coco; 4-Casca de pínus; 5-Casca de pínus + Fibra de coco (1:1; 6-Casca de arroz + Fibra de coco (1:1; 7-Casca de arroz carbonizada + Casca de pínus (1:1; 8-Casca de pínus + Casca de arroz carbonizada + Fibra de coco (1:1:1. O delineamento experimental foi o inteiramente casualizado com oito tratamentos e dez repeti

  11. Videojuego con Realidad Virtual

    OpenAIRE

    González Mora, César

    2017-01-01

    El objetivo del proyecto es el desarrollo de un videojuego deportivo que utilice realidad mixta. El videojuego se podrá utilizar con dispositivos de tipo cardboard, y utilizará realidad aumentada para la interacción del jugador con el videojuego. En el desarrollo se utilizará el motor Unity para conseguir una aplicación multiplataforma, y la librería Vuforia para implementar realidad mixta.

  12. Sistemas integrados con Arduino

    OpenAIRE

    EL YAKOUTI, MOHAMMED

    2017-01-01

    Design of a robot prototype remotely controllable from Bluetooth using Arduino. Control and testing of sensors and events interacting with Arduino and Bluetooth. Diseño de un prototipo de robot controlable remotamente con Bluetooth utilizando Arduino. Control y verificación de los sensores y eventos que interactúan mediante el Arduino y el Bluetooth. El Yakouti, M. (2017). Sistemas integrados con Arduino. http://hdl.handle.net/10251/89274. TFGM

  13. Correlation between heavy metal ions (copper, zinc, lead concentrations and root length of Allium cepa L. in polluted river water

    Directory of Open Access Journals (Sweden)

    Soraya Moreno Palacio

    2005-06-01

    Full Text Available The present work was performed using the common onion (Allium cepa L. as a bioindicator of toxicity of heavy metals in river water. The test waters were collected at two sampling sites: at the beginning and the end of the Toledo River. The bulbs of A. cepa L. were grown in test water with nine concentration levels of copper, zinc and lead from 0.1 to 50 ppm. In the laboratory, the influence of these test liquids on the root growth was examined during five days. For test liquids containing below 0.03-ppm dissolved Cu the root growth was reduced by 40% However, the same reduction occurred for 1-ppm dissolved Zn. For dissolved Pb, results reveal toxicity above 0.1 and 0.6 ppm at the beginning and the end of the Toledo river water, respectively.O presente trabalho foi realizado utilizando a cebola comum (Allium cepa L. como bioindicador da toxicidade de metais pesados em água de rio. As águas de teste foram coletadas em dois locais: na nascente e na foz do rio Toledo. Os bulbos de A. cepa L. foram cultivados em água de teste com nove níveis de concentração de cobre, zinco e chumbo de 0,1 a 50 ppm. Em laboratório a influência destes líquidos de teste em crescimento de raiz foi examinada durante cinco dias. Em todos os líquidos de teste o metal dissolvido contido foi medido pela técnica TXRF. Para líquidos de teste contendo 0,1-ppm de Cu dissolvido o crescimento da raiz foi reduzido em 50%. Entretanto, ocorreu a mesma redução para 1-ppm de Zn dissolvido. Para Pb dissolvido, o método do Allium teste revela toxidade acima de 0,1 e 0,5 ppm para a nascente e a foz do rio Toledo, respectivamente.

  14. Comportamento da cepa LE de Schistosoma mansoni, após passagem em hospedeiro humano infectado acidentalmente

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    Neusa Araújo

    1986-12-01

    Full Text Available Uma auxiliar de laboratório infectou-se acidentalmente, com cercárias de Schistosoma mansoni, cepa LE, mantida rotineiramente em nossos laboratórios. Decorridos 5 meses, o exame parasitológico de fezes revelou 108 ovos/g . A pacientefoi tratada com oxamniquine, porém a infecção continuou ativa (6 ovos/g. Foi então obtido o isolado SSF mantido no modelo Biomphalaria glabrata - camundongo albino. Os resultados obtidos no estudo comparativo, entre o isolado SSF e a cepa LE, que lhe deu origem, mostraram que a duração do período pré-patente e o índice de infectividade em camundongos, bem como a resposta aos agentes esquistossomicidas (hycanthone, oxamniquine epraziquantel não apresentaram diferenças estatisticamente significativas. Por outro lado, o número de miracídios obtidos dos intestinos e fígados dos camundongos infectados foi o dobro com a cepa LE, quando comparados com aquele do isolado SSF. Também a variação do peso dos animais foi bastante diferente. Concluiu-se que apenas uma passagem pelo hospedeiro humano não mudou substancialmente as características da cepa estudada.A laboratory technician was accidentally infected with the LE strain of Schistosoma mansoni cercariae maintained in our laboratories. After 5 months infection parasitological examination revealed 108 eggs/g faeces. Althrough treated with oxamniquine, the infection still persisted (6 eggs/g of faeces. An isolate (SSF was obtained from that person and maintained in Biomphalaria glabrata - Swiss mouse by alternate passage. The experimental comparative results between the newly obtained SSF isolate and the original LE strain showed that the differences in pre-patent period, the mice infectivity level and the schistosomicidal response to hycanthone, oxamniquine and praziquantel was not statistically significant. On the other hand the number of miracidia recovered from intestines and livers of equally infected mice was twice as miich in the LE strain than

  15. Serotipificación y susceptibilidad antibacteriana de cepas de Pseudomonas aeruginosa aisladas de pacientes quemados infectados

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    Aniel Moya

    2003-06-01

    Full Text Available Se aislaron 11 cepas de Pseudomonas aeruginosa en 10 pacientes quemados que se encontraban hospitalizados y se les realizó tipificación serológica y antibiotipia para determinar los serotipos más frecuentes en este tipo de pacientes. Los serotipos de mayor frecuencia de aislamiento fueron el O4 y el O5. Observamos una marcada resistencia frente a los antibióticos: carbenicilina (81,8% y la azlocilina (72,7%.

  16. Certain antioxidant enzymes of Allium cepa as biomarkers for the detection of toxic heavy metals in wastewater

    International Nuclear Information System (INIS)

    Fatima, Riffat A.; Ahmad, Masood

    2005-01-01

    The utility of antioxidant enzymes, viz glutathione-S-transferase (GST), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR), as biomarkers of heavy metal pollution in water was investigated using the Allium cepa (onion) system. These antioxidant enzymes were assayed in onion bulbs exposed to certain heavy metals taken separately, the test metals taken in combination as well as the industrial wastewater especially found to contain these metals. GST exhibited significantly enhanced activity upon treatment with individual heavy metals. However, GR, SOD and CAT did not show such a pronounced increase in activities. At higher heavy metal concentrations, GR, SOD and CAT showed a steep decline while GST activity still showed a rise. Moreover, APX, GPX and MDHAR also exhibited remarkable induction with increase in the concentration of individual heavy metals. However, there was no significant change in DHAR activity with respect to the controls. Metabolites like ascorbate (ASC) and glutathione (GSH) exhibited significant decline with increase in the concentration of individual heavy metals while the level of H 2 O 2 continued to display the rise up to a heavy metal concentration of 100 μM, after which it showed a gradual decline. A. cepa bulbs treated with wastewater sample showed enzyme activity profiles similar to that shown with heavy metals, thereby suggesting the presence of heavy metals in the test wastewater. Atomic absorption spectrophotometry also detected large amounts of Cd, Cr, Cu, Hg, Pb and Zn in the test water sample. The metal mixture, containing the amounts of heavy metals equivalent to those found in the wastewater, resulted in steep declines in GR, SOD and CAT activities in A. cepa while GST showed a rise. However, when this metal mixture was diluted to 2000-fold, GR, SOD and CAT also showed enhanced

  17. Quantification of Quercetin Obtained from Allium cepa Lam. Leaves and its Effects on Streptozotocin-induced Diabetic Neuropathy.

    Science.gov (United States)

    Dureshahwar, Khan; Mubashir, Mohammed; Une, Hemant Devidas

    2017-01-01

    Antioxidant potential has protective effects in diabetic neuropathy (DN); hence, the present study was designed with an objective to quantify quercetin from shade-dried leaves of Allium cepa Lam. and to study its effects on streptozotocin (STZ)-induced chronic DN. The shade-dried leaves of A. cepa Lam. were extracted with methanol and then fractionated using ethyl acetate (ACEA). The quantification of quercetin in ACEA was evaluated by high-performance thin layer chromatography (HPTLC). The STZ (40 mg/kg) was administered to Sprague-Dawley rats (180-250 g) maintained at normal housing conditions. The STZ was administered once a day for 3 consecutive days. The elevation in blood glucose was monitored for 3 weeks periodically using flavin adenine dinucleotide-glucose dehydrogenase method by Contour TS glucometer. Rats showing blood glucose above 250 mg/dl were selected for the study. Animals were divided into eight groups. ACEA (25, 50, and 100 mg/kg), quercetin (40 mg/kg), metformin (120 mg/kg), and gabapentin (100 mg/kg) were given orally once a day for 2 weeks. The blood glucose level was again measured at the end of treatment to assess DN. Thermal hyperalgesia, cold allodynia, motor incoordination, and neurotoxicity were studied initially and at the end of 2-week treatment. Biochemical parameters were also evaluated after 2-week drug treatment. The quercetin present in ACEA was 4.82% by HPTLC. All the ACEA treatment reduces blood glucose level at the end of the 2-week study and shows a significant neuroprotective effect in STZ-induced DN in the above experimental models. The quercetin present in ACEA proved protective effect in STZ-induced DN. High-performance thin layer chromatography reveals the presence of 4.82% quercetin in Allium cepa ethyl acetate. (ACEA). Its investigation against various diabetic neuropathy biomarkers has proved that ACEA has significant blood glucose reducing action shown neuroprotective action in thermal hyperalgesia, motor

  18. VERIFICACIÓN DEL CONTENIDO DE MATERIA SECA COMO INDICADOR DE COSECHA PARA AGUACATE (persea americana CULTIVAR HASS EN ZONA INTERMEDIA DE PRODUCCIÓN DE LOS SANTOS, COSTA RICA

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    María del Milagro Cerdas Araya

    2014-01-01

    Full Text Available El objetivo de esta investigación fue estu - diar si el contenido de materia seca (CMS de aguacate podría ser utilizado como un índice de cosecha adecuado en sustitución del contenido de aceite (CA en aguacate Hass, cultivado en una zona de altitud intermedia (1750 msnm, en Los Santos, Costa Rica. Se marcaron 100 árboles y un total de 2000 flores polinizadas; se evaluaron 35 frutos cada semana, de la semana 31 a la 44. Se encontró que ambos, CA y CMS, mostraron un aumento rápido y significativo después de la semana 39 (p≥0,05, con un coeficiente de correlación entre ellos de 0,92. Otros cambios observados simultáneamente, y relacionados con la maduración de la fruta, fueron el desarrollo del color morado en la cáscara (74% de la superficie, la reducción de la adherencia de la cubierta de la semilla a la pulpa de la fruta, y de ésta a la cáscara y a la semilla, así como la disminución de la firmeza del fruto, de 118 N durante la semana 31 hasta 57 N en la semana 40 y después de ésta. Se recomienda usar el 23% de CMS en el fruto de aguacate Hass como un índice de cosecha para el aguacate cultivado a 1750 msnm en la zona de Los Santos, como una alternativa al conteni - do de aceite de la fruta (CA.

  19. Investigando con personas con dificultades de aprendizaje

    Directory of Open Access Journals (Sweden)

    Borja González Luna

    2013-12-01

    Full Text Available El artículo muestra los orígenes de lo que Walmsley (2008 denomina «investigación inclusiva». Para comprender qué se entiende por investigación inclusiva tenemos que remontarnos a los debates epistemológicos sobre las metodologías cuantitativas y cualitativas, acontecidos en la década de los 90, en torno a la revista Disability & Society. A partir de una síntesis de dichos debates, focalizados en el ámbito de la «discapacidad intelectual y del desarrollo», se exponen dos estrategias de colaboración con dicha población: a una aproximación etnográfica (de trabajo grupal, y b una aproximación biográfica (de trabajo individual. A continuación se esboza un posible diseño de trabajo de campo que intenta superar el paradigma cualitativo «clásico» con el objetivo de incluir a dicho colectivo más allá del rol de «sujetos de la investigación». Para finalizar se recoge el debate sobre la accesibilidad de los resultados de la investigación a los participantes en dichas investigaciones, y con ello la necesaria innovación en el ámbito de las «devoluciones» de los resultados, cuando se trata de incluir a personas que presentan limitaciones para la comprensión del lenguaje abstracto oral y/o escrito.

  20. Variabilidad intraespecífica de cepas de Phytophthora palmivora Butler (Butler) aisladas de plantaciones de aguacatero (Persea americana Mill.) en Cuba

    OpenAIRE

    Machado, Maylen; Peña-Marey, Mabel; Alvarez, Alba; Díaz, Adriana; Zamora, Victoria; Coto, Orlando

    2013-01-01

    Se utilizaron en el estudio ocho cepas de Phytophthora palmivora Butler (Butler) obtenidas de jardines de aguacateros de la región occidental de Cuba, y se describieron los patrones de crecimientos de las colonias en medio agar papa dextrosa (PDA), agar zanahoria (AZ), agar jugo vegetal V8 (AV8) y agar malta (AM), así como los tipos de esporangios presentes. Se evaluó la variabilidad intraespecífica de los aislamientos en base a las dimensiones de los esporangios y de sus papilas. Las cepas m...

  1. Capacidad de invasión de los eritrocitos de pollo de una cepa de Mycoplasma synoviae aislada en Argentina

    OpenAIRE

    Uriarte, Javier; Cerdá, Raúl Oscar; Stanchi, Néstor Oscar

    2015-01-01

    Mycoplasma synoviae es uno de los patógenos más importantes en la industria de aves de corral y a menudo causa enfermedades de naturaleza crónica. Poco se sabe acerca de los mecanismos de persistencia en el hospedador y las cepas difieren significativamente en la invasividad y patogenicidad. Un estudio reciente demostró la capacidad de M. synoviae para invadir los eritrocitos de pollo (CER). El objetivo de este estudio fue determinar si una cepa de campo de M. synoviae, aislada de una granja ...

  2. Selección de Cepas de Hongos Entomopatógenos para el Manejo de Anastrepha obliqua (Macquart, 1835 (Diptera: Tephritidae en Colombia Selection of Strains of Entomopathogenic Fungi for Management of Anastrepha obliqua (Macquart, 1835 (Diptera: Tephritidae in Colombia

    Directory of Open Access Journals (Sweden)

    Armando Osorio-Fajardo

    2011-12-01

    Full Text Available Se evaluaron 15 cepas de los hongos entomopatógenos Beauveria bassiana y Metarhizium anisopliae sobre adultos de un día de edad de la mosca de la fruta Anastrepha obliqua. El trabajo se realizó con el fin de seleccionar las cepas más virulentas al insecto y estudiar el efecto sobre los adultos jóvenes cuando el hongo era aplicado antes de la emergencia. Mediante un screening con una concentración de 1x10(7 conidias/mL se seleccionaron las tres cepas más virulentas, siendo dos de ellas de Beauveria y una de Metarhizium, las cuales causaron mortalidades del 77%, 71% y 66%. Valores de CL50 de 2,38x10(6, 1,81x10(6 y 9,94x10(6 conidias/mL, respectivamente, fueron determinados para cada una de estas cepas y un TL50 respectivo de 48,12; 56 y 42,75 horas. No se encontraron diferencias significativas en la mortalidad entre hembras y machos. La aspersión de la CL90 de las cepas seleccionadas sobre el medio de pupación de la mosca de la fruta produjo 34-48% de mortalidad durante las 120 horas de evaluación. Los hongos entomopatógenos pueden ser utilizados fácilmente para el control biológico de A. obliqua aplicándolos de manera dirigida a los adultos jóvenes bajo la copa de los árboles, en programas de manejo integrado de plagas.Fifteen strains from entomopathogenic Beauveria bassiana and Metarhizium anisopliae fungi were evaluated on one day-old adults of Anastrepha obliqua fruit fly. Tested were carried out for selecting the most virulent strains and the effectiveness of their use on young adult when the entomopathogen were applied before emergence were studied too. A screening with a 1x10(7 conidia/mL concentration was used for selecting the three most pathogenic isolates, two from Beauveria and one from Metarhizium, having 77, 71 and 66% mortality. The LC50 for these isolates were 2.38x10(6, 1.81x10(6 and 9.94x10(6 conidia/mL, respectively, and a respective LT50 were 48.12, 56 and 42.75 hours. No significant differences were found

  3. Fiebre petequial experimental de las montañas rocosas: resultado del tratamiento con algunas drogas (1