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Sample records for con adenovirus implicaciones

  1. Condicionantes e implicaciones

    Directory of Open Access Journals (Sweden)

    Humberto González Galbán

    2005-01-01

    tiene como objetivo mostrar la problemática del embarazo adolescente en términos sociodemográficos relacionados con su diferente grado de manifestación, condicionantes e implicaciones sociales en el contexto cubano.

  2. Glomerulonefritis C3: una nueva categoría de glomerulonefritis con implicaciones etiopatogénicas

    OpenAIRE

    Taborda Murillo, Alejandra; Arroyave Suárez, Mariam José; Arias, Luis Fernando

    2015-01-01

    Introducción: las glomerulonefritis con depósitos exclusivos de la fracción C3 del complemento (GN-C3) pueden implicar alteración en la vía alterna de este. Objetivos: describir retrospectivamente una serie de casos de GN-C3 y determinar la frecuencia con que los pacientes continúan con alteraciones renales y/o hipocomplementemia. Métodos: se evaluaron las características histológicas y clínicas y la evolución de los 22 casos de GN-C3 diagnosticados entre 2004 y 2012 en el Departamento de Pat...

  3. APRENDIENDO A VIVIR CON EL ALUVIÓN INFORMATIVO Y SUS IMPLICACIONES PARA LA EDUCACIÓN

    Directory of Open Access Journals (Sweden)

    Barbara Jones

    2011-04-01

    Full Text Available Una característica importante de nuestra época es el aumento exponencial de los datos. Grandes cantidades de información, en formato digital, son consumidas para la investigación, y a la vez derivadas de la misma. Internet ha hecho la "información" accesible -no solo datos- a aquellas personas que tienen los recursos necesarios para acceder y utilizar Internet. Como resultado, se ha generado para las instituciones educativas un nuevo conjunto de requisitos para dotar a sus profesores y alumnos con habilidades para acceder y aprovechar efectivamente la información en este entorno digital, bien para propósitos de aprendizaje, o para alcanzar éxito en esferas personales o laborales. Este trabajo explora este nuevo paradigma, que proporciona un estudio del horizonte actual, con ejemplos de trabajos recientes donde se tratan estas áreas de interés, finalizando con la elaboración de conclusiones sobre el estado actual y las posibles direcciones futuras.

  4. Enfermedad óseo mineral relacionada con la enfermedad renal crónica: Klotho y FGF23; implicaciones cardiovasculares

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    Laura Salanova Villanueva

    2016-07-01

    Full Text Available Una de las principales causas de morbimortalidad en el paciente con enfermedad renal crónica es la cardiovascular. La inflamación y las alteraciones en el metabolismo óseo mineral son una condición patológica que conlleva aumento del riesgo cardiovascular en la enfermedad renal crónica. Los parámetros bioquímicos clásicos del metabolismo óseo mineral como fósforo, calcio, vitamina D y PTH tienen una implicación muy conocida en el riesgo cardiovascular. Los nuevos marcadores, FGF23 y klotho, también podrían estar implicados en la enfermedad cardiovascular.

  5. Implicaciones para el Ecuador del Tratado de Libre Comercio con Estados Unidos en relación a los medicamentos y a la salud.

    OpenAIRE

    Logroño Barrionuevo, Milton Efraín

    2006-01-01

    1. LEGISLACION DE PROPIEDAD INTELECTUAL 2.EL TRATADO DE LIBRE COMERCIO: MEDICAMENTOS Y SALUD 3. IMPACTO DEL TRATADO DE LIBRE COMERCIO EN MEDICINAS Y SALUD.4. IMPLICACIONES SOCIALES DEL TRATADO DE LIBRE COMERCIO EN MEDICAMENTOS Y SALUD 5. PROPUESTA DE NEGOCIACIÓN EQUITATIVA DEL ECUADOR EN EL TRATADO DE LIBRE COMERCIO EN MEDICAMENTOS Y SALUD.6. CONCLUSIONES Y RECOMENDACIONES

  6. Crecimiento, acumulación y empleo en una economia con tasa de inflación constante: implicaciones de política económica

    OpenAIRE

    Luis Alberto Alonso González; Jorge Uxó González

    1995-01-01

    Análisis teórico del problema del desempleo elevado y persistente que caracteriza a la mayoría de las economías europeas. Se hace un enfoque dinámico, y al final del artículo se extraen las implicaciones de política económica más relevantes.

  7. Dificultades del alumnado con déficit de atención con hiperactividad (TD?? en el aula: implicaciones para la formación docente

    Directory of Open Access Journals (Sweden)

    Francisco D. FERNÁNDEZ MARTÍN

    2009-11-01

    Full Text Available RESUMEN: El propósito del estudio fue determinar el impacto de una intervención cognitivo-conductual sobre una muestra de alumnos diagnosticados con TDAH pertenecientes a diversos centros de enseñanza de Educación Primaria de la provincia de Granada. Para llevar a cabo el estudio, se utilizaron diversas pruebas con las que se evaluó el control inhibitorio, el control perceptivomotor, la memoria y la atención, considerados/ as como los/as manifestaciones más comunes del TDAH, en el contexto escolar. La intervención se basó en la formación de padres y docentes, así como en un posterior entrenamiento específico y directo sobre el alumnado. Los resultados post de la intervención, manifiestan que se han producido mejoras significativas en las conductas problema que presentaban los alumnos en el contexto escolar.ABSTRACT: The purpose of this study was to determine the impact of a cognitive-behavioural intervention among a sample of students diagnosed with ADHD, belonging to several Primary Education Schools within the province of Granada. In order to carry out this research several evaluation test were administered to evaluate inhibitory control, motor-perceptive control, memory and attention, considered those as the more frequent features of ADHD, within the school settings. The intervention consisted of providing training teachers. The Post intervention results show that significant improvement where reached by students at school settings.RÉSUMÉ: L'intention de l'étude c'était déterminer l'impact d'une intervention cognitive-conductuel sur une échantillon d'élèves diagnostiqués avec TDAH, appartenant à divers centres d'enseignement d'Éducation Primaire de la province de Granada. Pour réaliser l'étude, on a utilisé des divers preuves avec les quelles on a évalué le contrôle inhibitoire, le contrôle perceptive-moteur, la mémoire et l'attention considérés comme les manifestations les plus communes du TDAH, dans le

  8. Diferencias en la agregación plaquetaria de sangre coronaria y periférica de pacientes con enfermedad coronaria. Implicaciones clínicas

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    Jaime Cabrales, MD

    2010-11-01

    Conclusión: Se describe la presencia de mayor agregación plaquetaria en el seno coronario de pacientes con enfermedad aterosclerótica, la cual es significativa para ADP, colágeno y epinefrina. Se sugiere la aparición de factores locales asociados con la enfermedad coronaria que aumentan la agregación plaquetaria. La agregación plaquetaria periférica no refleja el comportamiento local en pacientes con aterosclerosis coronaria.

  9. Implicaciones de las politicas de conservación ambiental en la relación de los habitantes de Guasca con su entorno

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    Helber Mauricio Bernal Moreno

    2017-09-01

    Full Text Available Dentro de la agenda de algunos Gobiernos nacionales se ha hecho énfasis en la importancia de luchar contra el cambio climatico y de proteger los recursos naturales, razón por la cual han desarrollado politicas estatales que permitan ejercer un mayor control sobre estos recursos y garantizar su preservación y cuidado. Estas politicas han considerado viable que dicha protección se pueda incentivar a través de la inversión económica de actores privados (bancos, ONG, entidades ambientalistas, entre otros. No obstante, al insertar la conservación de los recursos naturales en una lógica de costo/beneficio, se genera que se desarrollen dinamicas de acaparamiento donde las personas con mayor poder adquisitivo deciden cómo y de qué forma se usan estos recursos. Al pensar en el poblador local como un depredador que solo extrae recursos de su entorno, se desconoce la relación histórica de este con la naturaleza, donde se han creado y fortalecido elementos sensoriales y materiales. El municipio de Guasca no es ajeno a estas politicas de conservación ambiental y, si bien pueden estar pensadas para proteger la naturaleza, tienden a desconocer a las personas que durante muchos anos han convivido con esta (campesinos y pobladores locales. Asi mismo, resulta contradictorio que quienes desarrollan estas politicas muchas veces pretenden darle usos simultaneos a la naturaleza, aunque sean contrapuestos. De este modo se observan lugares en Guasca que estan proyectados (por entes territoriales, corporaciones ambientales y entidades privadas para la conservación de fuentes hidricas, fauna y flora en simultanea con proyectos extractivistas minero-energéticos.

  10. Activated recombinant adenovirus proteinases

    Science.gov (United States)

    Anderson, Carl W.; Mangel, Walter F.

    1999-08-10

    This application describes methods and expression constructs for producing activatable recombinant adenovirus proteinases. Purified activatable recombinant adenovirus proteinases and methods of purification are described. Activated adenovirus proteinases and methods for obtaining activated adenovirus proteinases are further included. Isolated peptide cofactors of adenovirus proteinase activity, methods of purifying and identifying said peptide cofactors are also described. Antibodies immunoreactive with adenovirus proteinases, immunospecific antibodies, and methods for preparing them are also described. Other related methods and materials are also described.

  11. Implicaciones del tratamiento antirretroviral en la disfunción sexual del paciente con infección por el virus de la inmunodeficiencia humana.

    OpenAIRE

    Cánoves Martínez, Mª Dolores

    2007-01-01

    RESUMEN Los años 80 fueron el inicio conocido de los primeros casos de VIH y ya desde entonces, se describió el origen de la enfermedad como la consecuencia de los estilos de vida de los afectados. La información facilitada en este sentido, contribuyó a la creación de estereotipos; que con variantes, siguen hasta la actualidad; De esta forma, es habitual que los pacientes sufran además de los problemas biológicos, los psicológicos y los sociales asociados, característica ...

  12. Implicaciones de las emociones en la evaluación de la calidad y satisfacción de los servicios universitarios: una experiencia de caso con estudiantes

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    Nubia Consuelo Cortés Rodríguez

    2014-12-01

    Full Text Available En la última década, las universidades colombianas han emprendido procesos de mejoramiento para lograr el reconocimiento de alta calidad en sus programas académicos, condiciones que influyen en los estudiantes o usuarios potenciales para seleccionar el programa de formación profesional y garantizar su permanencia y terminación de estudios universitarios. El Estado colombiano, a través del Ministerio de Educación, tiene la responsabilidad de vigilar y controlar la calidad en la oferta de programas universitarios. El estudiante es el usuario que percibe de forma directa la calidad de la educación en el proceso de formación; sin embargo, no hay escalas o instrumentos disponibles que permitan la medición objetiva sobre la percepción de la calidad, emoción y satisfacción en la educación superior. La presente investigación pretende diseñar y validar un instrumento que permita establecer la relación entre las variables de calidad, emoción y satisfacción en los estudiantes universitarios, caso de aplicación en el programa de pregrado en Administración Turística y Hotelera de la Universidad Pedagógica y Tecnológica de Colombia, sede Duitama (Boyacá. El instrumento diseñado fue dividido en tres secciones: calidad, emoción y con una única pregunta en satisfacción; se realizó una prueba piloto con un valor de alpha de Cronbach de 0.821 para calidad y 0.929 para emociones, confirmando la consistencia interna del instrumento y permitiendo su aplicación a la población seleccionada por muestreo aleatorio simple. A los resultados del instrumento en calidad y emoción se les aplicó el análisis de componentes principales y un análisis factorial para obtener un menor número de variables relacionadas. Posteriormente se propuso un modelo estadístico de regresión categórica, para determinar la influencia de las emociones y la calidad en la satisfacción de los estudiantes. Se utilizó el programa estadístico SPSS versión 17.

  13. Las implicaciones macroeconómicas de las nuevas regulaciones bancarias con respecto al capital en mercados emergentes: un modelo de duopolio adaptado a bancos aversos al riesgo.

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    Sebastian Nieto Parra

    2010-05-01

    Full Text Available Con el propósito de analizar el impacto de la nueva regulación de capital de bancos (Basilea II sobre el ciclo económico de una economía emergente, desarrollo un modelo de duopolio compuesto por bancos locales y extranjeros. Los principales resultados son: por medio de la nueva regulación de capital, la evaluación del riesgo crediticio realizada por un banco internacional en un país, no sólo afecta a los préstamos totales de ese país sino también a los activos totales otorgados en otros países. Segundo, cuando los bancos son aversos al riesgo y a medida que la diversificación del portafolio aumenta, el cambio en los préstamos concedidos en un país por un banco internacional como proporción de la inversión inicial, así como el nivel de los prestamos totales de ese país, pueden resultar fuertemente afectados por el comportamiento de un banco que sigue sólo “noticias” a través de la nueva regulación de capital. Finalmente, incluso cuando la diversificación del portafolio crece sin  límite, la implicación macroeconómica de un cambio en la estimación del riesgo crediticio debida a la nueva regulación de capital, aumenta a medida que los bancos son menos aversos al  riesgo.

  14. Dispepsia funcional: Nuevos conocimientos en la fisiopatogenia con implicaciones terapéuticas Functional dyspepsia: New pathophysiologic knowledge with therapeutic implications

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    Ana C. Hernando-Harder

    2007-08-01

    Full Text Available La dispepsia funcional (DF es un complejo sintomático, heterogéneo y altamente prevalente en la comunidad y en la práctica general. La DF se define como la presencia de síntomas que se piensan originados en la región gastroduodenal, en ausencia de enfermedad orgánica, sistémica o metabólica que pueda explicarlos. Entre los factores fisiopatogénicos se incluyen los trastornos de la acomodación y del vaciamiento gástrico, dismotilidad duodenal, sensibilidad aumentada, factores psicosociales y una asociación con un estado postinfeccioso. Se han hecho numerosos esfuerzos para aumentar los conocimientos en la etiopatogenia del síndrome, incluyendo nuevos aspectos moleculares y genéticos. Sin embargo, el mecanismo etiopatogénico exacto que causa los síntomas en un paciente individual sigue siendo difícil de identificar. Los nuevos criterios de Roma III redefinen y subclasifican la DF basándose en sus síntomas principales, lo cual es de gran valor para la investigación, el desarrollo y el control estandarizados de nuevas estrategias terapéuticas así como la formulación de recomendaciones para la práctica clínica. Las modalidades terapéuticas que se han empleado incluyen: modificaciones dietéticas, agentes farmacológicos dirigidos a actuar sobre distintos blancos dentro del aparato gastrointestinal, del sistema nervioso central y periférico, y terapias psicológicas incluyendo la hipnoterapia. Desafortunadamente, hasta la fecha, todas estas terapias han rendido solamente resultados marginales. Después de excluir enfermedad orgánica, es esencial que el paciente esté informado sobre la naturaleza y el pronóstico benignos de su enfermedad, y esto puede ser, a veces, la inversión más provechosa tanto para el paciente como para su médico.Functional dyspepsia (FD is a heterogeneous, highly prevalent symptom complex in the community and general practice. FD is defined as the presence of symptoms considered as originated in

  15. Interferon induction by adenoviruses

    Energy Technology Data Exchange (ETDEWEB)

    Beladi, I; Bakay, M; Pusztai, R; Mucsi, I; Tarodi, B [University Medical School, Szeged (Hungary). Inst. of Microbiology

    1979-02-01

    All human, simian, bovine and avian adenovirus types tested so far and the canine hepatitis virus induce interferon production in chick cells. This finding indicated this property to be characteristic for viruses belonging to the adenovirus group. Trypsin treatment, which had no effect upon the infectivity, diminished or eliminated the interferon-inducing abilities of crude adenoviruses, and thus the need for a trypsin-sensitive protein in interferon induction was suggested. T antigen and interferon were formed simultaneously in chick embryo fibroblast cells infected with human adenovirus type 12, and there-fore the adenovirus-specific T antigen was resitant to the action of endogenous interferon synthetized by the same cells. In chicks inoculated with human types, the appearance of interferon was biphasic: an 'early' and a 'late' interferon could be demonstrated with maximum titre 4 and 10 hr, respectively, after virus infection. In chicks infected with adenoviruses, first interferon production and then a decreased primary immune response to sheep red blood cells was observed. It was assumed that in adenovirus-infected chicks the interferon produced by viral stimulus resulted in a transient immunosuppression.

  16. IMPLICACIONES BIOÉTICAS Y BIOJURÍDICAS DE LA OBJECIÓN DE CONCIENCIA INSTITUCIONAL CON RELACIÓN AL ABORTO EN EL ORDENAMIENTO JURÍDICO COLOMBIANO

    Directory of Open Access Journals (Sweden)

    Amparo de Jesús Zárate Cuello

    2011-01-01

    Full Text Available El comienzo de la vida humana se encuentra en vilo en Colombia, ante la ratificación del Protocolo Facultativo sobre la eliminación de todas las formas de discriminación contra la mujer, adoptada por la Asamblea General de las Naciones Unidas de 1999, ratificada en el año de 2005. El tema se desarrolla en dos Sentencias, la primera, C-355 de 2006 despenaliza el aborto en tres situaciones excepcionales: Violación, enfermedad de la madre y malformación del feto. La segunda, T-388 de 2009 abarca la objeción de conciencia, que solamente es reconocida a las personas naturales; afirmando el máximo Tribunal Constitucional que no es un derecho del cual sean titulares las personas jurídicas o el Estado, limitando a las clínicas, hospitales y centros de salud que presenten objeción de conciencia para la no realización de la práctica del aborto. El no cumplimiento de esta declaración ha producido sanciones a las instituciones sanitarias privadas por el no acatamiento de los efectos erga ommes de las sentencias proferidas por la Corte Constitucional; estas instituciones reclaman objeción de conciencia institucional por su ideario y creencias religiosas. Estos criterios tienen implicaciones bioéticas y biojurídicas frente a la interpretación pluralista de orden filosófico, religioso y jurídico del artículo 18 de la Carta Política; el cual, garantiza la libertad de conciencia en nuestro Estado Social de Derecho y, es el legislador quien debe reasumir su competencia a través del bioderecho.

  17. Implicaciones del endotelio en la insuficiencia cardiaca

    Directory of Open Access Journals (Sweden)

    Beatriz Wills Sanín

    2014-10-01

    Full Text Available El papel del endotelio en la insuficiencia cardíaca (IC ha sido el centro de intensa investigación, puesto que tiene importantes implicaciones sistémicas expresadas en su efecto vasomotor, hemostático e inflamatorio. Existen diferencias individuales en el patrón de la disfunción endotelial de acuerdo a la etiología y severidad de la IC. La disfunción endotelial tiene un importante valor pronóstico en la IC y, aunque aún necesitamos conocer más aspectos de relevancia clínica, es evidente que esta condición se relaciona con desenlaces negativos en estos pacientes. Estamos a la espera de nuevos agentes farmacéuticos dirigidos específicamente al endotelio vascular. En esta revisión, nuestro objetivo es ofrecer un análisis crítico y contemporáneo de la evidencia disponible sobre las consecuencias patológicas y pronóstico de las anomalías endoteliales que definen el inicio, la historia natural y, en última instancia, los desenlaces en la IC.

  18. Diseño experimental de un sumidero de CO2 y sus implicaciones en el cambio climático. Una experiencia de trabajo con alumnos en el laboratorio de Educación Secundaria

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    Raquel Boronat-Gil

    2018-01-01

    Full Text Available El desarrollo teórico del cambio climático en el aula de Educación Secundaria requiere de experiencias recreativas que demuestren –in situ– diferentes aspectos del proceso global. La presente comunicación muestra una actividad de laboratorio, y el consiguiente trabajo del alumnado, para comprobar la reducción de gases de efecto invernadero mediante la aplicación de una tecnología básica, de bajo costo y que puede realizarse con materiales disponibles en cualquier laboratorio escolar. La analogía didáctica llevada a cabo entre el CO2 producido por el metabolismo microbiano dentro de una botella cerrada y su reacción con una base fuerte generadora de una presión inferior a la atmosférica (como consecuencia de la reacción del dióxido de carbono con una base, visible con un sencillo manómetro en U, será objeto de debate entre profesor-alumno, analizándose y especulando su captura con la repercusión a nivel mundial de mejora en el calentamiento global.

  19. Caracterización de la neuroinflamación en modelos animales y en pacientes con encefalopatía hepática. Implicaciones terapéuticas

    OpenAIRE

    Balzano, Tiziano

    2017-01-01

    Varios millones de personas afectadas por enfermedades hepáticas crónicas (cirrosis, hepatitis) muestran alteraciones neurológicas, denominadas encefalopatía hepática (HE) con alteraciones cognitivas y motoras que deterioran la calidad de vida y reducen la esperanza de vida. La carga económica de la encefalopatía hepática es sustancial, con un costo estimado entre mil y siete mil millones de dólares por año en los EE.UU. La patogénesis de la encefalopatía hepática y los mecanismos que cond...

  20. Resultado de las quemaduras tratadas mediante cultivo de células epidérmicas autólogas : reglamentos e implicaciones para ensayos clínicos internacionales con Productos de Terapia Avanzada

    NARCIS (Netherlands)

    Middelkoop, E.; Gardien, K.L.M.; Brouwer, K.M.; Waaijman, T.; Uhlrich, M.M.W.; Gibbs, S.; Ulrich, M.M.W.

    2016-01-01

    Introducción. Los Productos de Terapia Avanzada (ATMPs) son considerados el siguiente paso para mejorar la evolución de las quemaduras. Sin embargo, existen serios obstáculos para su aplicabilidad con respecto al reglamento internacional vigente. Aparte de las exigencias reglamentarias para los

  1. Adenovirus (For Parents)

    Science.gov (United States)

    ... by sharing contaminated objects (such as towels or toys), or by touch. Once a child is exposed to adenovirus, symptoms usually develop from ... washing, keep shared surfaces (such as countertops and toys) clean, and remove kids ... a week your child has breathing problems your child is under 3 ...

  2. LEARNING TO LIVE WITH DATA DELUGE AND WHAT THAT MEANS FOR EDUCATORS APRENDIENDO A VIVIR CON EL ALUVIÓN INFORMATIVO Y SUS IMPLICACIONES PARA LA EDUCACIÓN

    Directory of Open Access Journals (Sweden)

    Barbara Jones

    2011-03-01

    Full Text Available A major characteristic of our age is the exponential rise of data. Vast quantities of information in digital form are both consumed by and generated from research. The Internet has made “information” accessible to those with the resources to access and use the Internet. As a consequence a new set of requirements has been generated for educational institutions to equip both their teachers and learners with the information skills to access and effectively exploit this digital environment for learning purposes and for success in employment and personal spheres. This short paper explores this new paradigm providing a review of the current landscape including examples of recent work which addresses these areas, and drawing some conclusions about current status and possible future directions.Una característica importante de nuestra época es el aumento exponencial de los datos. Grandes cantidades de información, en formato digital, son consumidas para la investigación, y a la vez derivadas de la misma. Internet ha hecho la "información" accesible -no solo datos- a aquellas personas que tienen los recursos necesarios para acceder y utilizar Internet. Como resultado, se ha generado para las instituciones educativas un nuevo conjunto de requisitos para dotar a sus profesores y alumnos con habilidades para acceder y aprovechar efectivamente la información en este entorno digital, bien para propósitos de aprendizaje, o para alcanzar éxito en esferas personales o laborales. Este trabajo explora este nuevo paradigma, que proporciona un estudio del horizonte actual, con ejemplos de trabajos recientes donde se tratan estas áreas de interés, finalizando con la elaboración de conclusiones sobre el estado actual y las posibles direcciones futuras.

  3. Crecimiento, acumulación y empleo en una economía con tasa de inflación constante : implicaciones de política económica

    OpenAIRE

    Uxó González, Jorge

    1994-01-01

    Los objetivos fundamentales de la tesis son analizar que tipo de restricciones impiden a las economías de mercado creer a un ritmo suficiente para evitar la aparicion de desempleo, y estudiar la influencia de la politica económica sobre dichas restricciones. En la primera parte se define y obtiene la tasa de crecimiento no aceleradora de la inflación. En la segunda parte se estudia la dinámica de la economía suponiendo que la tasa de crecimiento efectiva coincide siempre con la no aceleradora...

  4. Adultos com fraturas: das implicações funcionais e cirúrgicas à educação em saúde Adultos con fracturas: de las implicaciones funcionales y quirurgicas hasta la educación para la salud Fractures in adults: from functional and surgical implications to health education

    Directory of Open Access Journals (Sweden)

    Luciana Tokunaga Itami

    2009-12-01

    Full Text Available Este estudo objetivou identificar as implicações funcionais e cirúrgicas relativas a pacientes adultos com fraturas, caracterizar o perfil sociodemográfico da amostra do estudo, avaliar a independência funcional das vítimas de fraturas e discutir as implicações relacionadas aos procedimentos cirúrgicos envolvidos no tratamento a esses pacientes. A pesquisa foi realizada entre novembro de 2006 e abril de 2007 com 74 pacientes internados em um hospital de São Paulo. Foram predominantes indivíduos do sexo masculino (91,9%, média de idade de 31,8 anos, brancos (51,3%, vítimas de acidentes de trabalho (51,4%. As fraturas de membros inferiores representaram 73,0% dos casos e membros superiores 13,5%. Os acidentes automobilísticos foram responsáveis por 58,1% das internações. Quanto à Medida de Independência Funcional (MIF, houve aumento nas médias dos valores no decorrer da avaliação. Por outro lado, há diminuição desses valores em casos de acidentes automobilísticos e atropelamentos e naqueles em que os membros inferiores são atingidos.Este estudio tuvo como objetivo identificar las implicaciones funcionales y quirúrgicas de adultos con fracturas, caracterizar el perfil demográfico de la muestra del estudio, evaluar la independencia funcional de las víctimas de fracturas y discutir las implicaciones relativas a los procedimientos quirúrgicos implicados en el tratamiento de estos pacientes. La encuesta fue realizada entre noviembre de 2006 y abril de 2007 con 74 pacientes ingresados en un hospital de Sao Paulo. Varones fueron predominantes (91,9%, edad promedio 31,8 años, blancos (51,3%, víctimas de accidentes (51,4%. Las fracturas de extremidades inferiores representaron el 73,0% de los casos y de extremidades superiores 13,5%. Los accidentes fueron responsables por 58,1% de los ingresos. Cuanto a la Medida de Independencia Funcional (MIF, hubo un aumento en los valores medios durante la evaluación. Por otra parte

  5. Co-factor activated recombinant adenovirus proteinases

    Science.gov (United States)

    Anderson, Carl W.; Mangel, Walter F.

    1996-08-06

    This application describes methods and expression constructs for producing activatable recombinant adenovirus proteinases. Purified activatable recombinant adenovirus proteinases and methods of purification are described. Activated adenovirus proteinases and methods for obtaining activated adenovirus proteinases are further included. Isolated peptide cofactors of adenovirus proteinase activity, methods of purifying and identifying said peptide cofactors are also described. Antibodies immunoreactive with adenovirus proteinases, immunospecific antibodies, and methods for preparing them are also described. Other related methods and materials are also described.

  6. Contact with hospital syringes containing body fluids: implications for medical waste management regulation Jeringas en contacto con sangre y fluidos corporales utilizadas en el hospital: implicaciones para el manejo de desechos hospitalarios

    Directory of Open Access Journals (Sweden)

    Patricia Volkow

    2003-04-01

    Full Text Available OBJECTIVE: To determine amount of syringes used in the hospital and extent of contact with blood and body fluids of these syringes. MATERIAL AND METHODS: Syringe use was surveyed at a tertiary care center for one week; syringes were classified into the following four categories according to use: a contained blood; b contained other body fluids (urine, gastric secretion, cerebrospinal fluid, wound drainage; c used exclusively for drug dilution and application in plastic intravenous (IV tubes, and d for intramuscular (IM, subcutaneous (SC, or intradermic (ID injections. RESULTS: A total of 7 157 plastic disposable syringes was used; 1 227 (17% contained blood during use, 346 (4.8%, other body fluids, 5 257 (73% were used exclusively for drug dilution and application in plastic IV lines, and 327 (4.5% were utilized for IM, SC, or ID injections. An estimated 369 140 syringes used annually, or eight syringes per patient per in-hospital day. All syringes were disposed of as regulated medical waste, in observance of the law. CONCLUSIONS: There is an urgent need to review recommendations for medical waste management by both international agencies and local governments, based on scientific data and a cost-benefit analysis, to prevent resource waste and further environmental damage.OBJETIVO: Cuantificar el número de jeringas que se utilizan en el hospital y calcular cuántas de éstas entran en contacto con sangre o fluidos corporales. MATERIAL Y MÉTODOS: Se hizo una encuesta del uso de jeringas en un hospital de tercer nivel de atención durante toda una semana. Se clasificaron, de acuerdo con el uso que se les dio, en cuatro categorías: a aspiración de sangre, b otros fluidos corporales (orina, secreción gástrica, líquido cefalorraquídeo, drenaje de herida, etcétera, c uso exclusivo para diluir medicamentos y administrarlos a través de tubos de terapia intravenosa, d para aplicación de inyecciones intramusculares (IM, subcutáneas (SC o

  7. Implications of energy reform on Mexican legislation for new and conventional energy technologies; Implicaciones de la reforma energetica sobre la legislacion mexicana con respecto a tecnologias energeticas nuevas y convencionales

    Energy Technology Data Exchange (ETDEWEB)

    Bautista Rodriguez, C. Moises; Tapia Pachuca, A. Belen [Alter Energias Grupo, Puebla (Mexico). E-mail: celso.bautista@thyssenkrupp.com; Gaytan Cruz, Veronica; Rivera Marquez, J. A.; Marin Torres, J. Lucina (Benemerita Universidad Autonoma de Puebla, Puebla (Mexico)

    2009-09-15

    The current economic-political conditions in the oil market are spurring on future alternative energy models worldwide, one of which is fuel batteries. This technology is foreseen to have several applications in portable and stationary services. Previous studies have reported on the slowness of Mexican legislation, compared to international legislation, on the collection and treatment of spent domestic batteries. Legislation on the issue is limited to a project for a Non-Mandatory Mexican Norm proposed since 2006, without having been implemented at the time the present document was written. In addition to the toxic environmental impact of the lack of regulation, it also represents a waste of energy, impacting the finances of users who throw away batteries with a significant residual energy charge (>60 %). This work analyzes recent Mexican energy reform to regulate the use of alternative technologies. Compared to international legislation, the basis of the Mexican legislation is unclear and incomplete, lacking technological consulting from governmental scientific institutions and clear, complete legislation adhering to international principles for the environment and society. These characteristics pose a risk to regulations and their application in Mexican society, and involve a loss in the benefits of applying clean technologies having better performance, a continued reflection of slow technological, economic, environmental and social advancement. [Spanish] En la actualidad, las condiciones economico-politicas en el mercado del petroleo estan permitiendo la busqueda de alternativas para el futuro modelo energetico mundial, una opcion es impulsar las pilas a combustible como una alternativa para el futuro energetico. Esta tecnologia preve tener multiples aplicaciones en servicios portatiles y estacionarios. En estudios previos se ha presentado el letargo de la legislacion mexicana con respecto a la legislacion internacional sobre el tema de recoleccion y tratamiento

  8. ¿Cómo afecta la unidad espacial a la visualización y modelado del área de mercado con sistemas de información geográfica? Implicaciones para el geomarketing

    Directory of Open Access Journals (Sweden)

    Moreno Jiménez, Antonio

    2004-12-01

    Full Text Available In geomarketing, mapping and modelling market areas, using GIS and statistical techniques, are common tasks when approaching spatial decision problems by retail and service firms. To this end, concepts and data concerning to demand (e.g. penetration rate, market share, sales per customer, etc., referred to spatial units, are used. Based on previous evidence on the so-called modifiable spatial unit problem, in this paper it is explored how the cartographic representation and modelling of market area are affected when different spatial units are used. The conclusions allow to apprehend the scope of such effects, provide suggestions for a more thoughtful choice of spatial units when GIS are applied, and, lastly, help to improve decision making on new store location through the analog approach.

    Dentro del geomarketing, y en aras de solventar problemas de decisión espacial por parte de las empresas comerciales y de servicios a escala local, una de las tareas comunes estriba en cartografiar y modelar las áreas de mercado, combinando la tecnología SIG con técnicas estadísticas. A tal fin se parte de conceptos y datos relativos a la demanda (por ejemplo, tasa de penetración, cuota de mercado, gasto per cápita, etc. referidos a determinadas unidades espaciales. Ante las evidencias que estudios previos han obtenido sobre el llamado problema de la unidad espacial modificable (PUEM, en este trabajo se investiga cómo se ve afectada la representación y modelado del área mercado cuando se adoptan conjuntos distintos de unidades espaciales. Las conclusiones permiten valorar el alcance de dichos efectos, proporcionan recomendaciones para elegir de manera más atinada las unidades espaciales a la hora de aplicar los SIG y coadyuvan, en última instancia, a mejorar la toma de decisiones sobre localización de nuevos establecimientos mediante el método de la analogía.

  9. Transformation and oncogenicity by Adenoviruses

    NARCIS (Netherlands)

    Bernards, R.A.; Eb, A.J. van der

    1984-01-01

    Adenoviruses have attracted considerable attention since it was discovered by TRENTIN et all. and HUEBNER et al. that certain species (formerly called serotypes) are oncogenic when injected into newborn hamsters. Since then, adenoviruses have been used extensively as a model for studies on tumor

  10. Enfermedad neurologica por adenovirus Neurologic disease due to adenovirus infection

    Directory of Open Access Journals (Sweden)

    Cristina L. Lema

    2005-06-01

    Full Text Available El objetivo de este trabajo fue determinar la prevalencia de adenovirus (ADV en las infecciones del sistema nervioso central (SNC. Se analizaron 108 muestras de líquido cefalorraquídeo (LCR provenientes de 79 casos de encefalitis, 7 meningitis y 22 de otras patologías neurológicas, recibidas en el período 2000-2002. Cuarenta y nueve (47.35% se obtuvieron de pacientes inmunocomprometidos. La presencia de ADV se investigó mediante reacción en cadena de la polimerasa en formato anidado (Nested-PCR. La identificación del genogrupo se realizó mediante análisis filogenético de la secuencia nucleotídica parcial de la región que codifica para la proteína del hexón. Se detectó la presencia de ADV en 6 de 108 (5.5% muestras de LCR analizadas. Todos los casos positivos pertenecieron a pacientes con encefalitis que fueron 79, (6/79, 7.6%. No se observó diferencia estadísticamente significativa entre los casos de infección por ADV en pacientes inmunocomprometidos e inmunocompetentes (p>0.05. Las cepas de ADV detectadas se agruparon en los genogrupos B1 y C. En conclusión, nuestros resultados describen el rol de los ADV en las infecciones neurológicas en Argentina. La información presentada contribuye al conocimiento de su epidemiología, en particular en casos de encefalitis.The aim of this study was to assess the prevalence of adenovirusm (ADV infections in neurological disorders. A total of 108 cerebrospinal fluid (CSF samples from 79 encephalitis cases, 7 meningitis and 22 other neurological diseases analysed in our laboratory between 2000 and 2002 were studied. Forty nine (47.4% belonged to immunocompromised patients. Viral genome was detected using nested polymerase chain reaction (Nested-PCR and ADV genotypes were identified using partial gene sequence analysis of hexon gene. Adenovirus were detected in 6 of 108 (5.5% CSF samples tested. All of these were from encephalitis cases, 6/79, representing 7.6% of them. No statistically

  11. Pessoas acima de 50 anos com aids: implicações para o dia-a-dia Personas con más de 50 años que tienen sida: implicaciones para el cotidiano People with more than 50 years old with aids: implications to everyday life

    Directory of Open Access Journals (Sweden)

    Soraia Romera Machiesqui

    2010-12-01

    Full Text Available Este estudo caracteriza-se por ser uma pesquisa qualitativa que objetivou descrever as implicações relacionadas ao dia-a-dia de pessoas acima de 50 anos que têm a síndrome da imunodeficiência adquirida. A pesquisa foi realizada num hospital de ensino na região sul do Brasil. A produção dos dados foi desenvolvida com a dinâmica de criatividade e sensibilidade Mapa Falante, por um grupo de cinco participantes. Para a análise, foi aplicada a técnica de análise temática do conteúdo. Das produções artísticas e depoimentos, emergiram os efeitos decorrentes da descoberta do diagnóstico; da compreensão de que tem uma vida normal apesar da doença; da fé em Deus; do preconceito e discriminação e do silêncio da condição sorológica. Conclui-se que as ações de cuidado devem contemplar as dimensões biológicas, clínica, social e subjetiva de modo corresponsável vislumbrando a autonomia para o cuidado com a sua saúde e para as escolhas de sua vida.Investigación cualitativa que tuvo por objetivo describir las implicaciones relacionadas al cotidiano de personas con más de 50 años de edad que tienen el síndrome de inmunodeficiencia adquirida. Realizada en hospital de enseñanza en la región sur de Brasil. La producción de los datos fue desarrollada con dinámica de creatividad y sensibilidad mapa hablante, por un grupo de cinco participantes. Para el análisis, fue aplicada la técnica de análisis temático del contenido. De las producciones artísticas y declaraciones emergieron los efectos decurrentes de la descubierta del diagnóstico; de la comprensión de que tiene una vida normal a pesar de la enfermedad; de la fe en Dios; del prejuicio y discriminación y del silencio de la condición serológica. Se concluye que las acciones de cuidado deben contemplar las dimensiones biológicas, clínica, social y subjetiva de modo co-responsable vislumbrando la autonomía para el cuidado con su salud y para las elecciones de su

  12. Aspectos fisiológicos del catión cinc y sus implicaciones cardiovasculares

    OpenAIRE

    Díaz García, Carlos Manlio; Álvarez González, Julio L.

    2009-01-01

    Introducción La versatilidad del catión cinc en la fisiología de diversos tipos celulares ha conllevado a una creciente atención de la comunidad científica sobre sus roles funcionales y la regulación a la que se encuentran sus niveles. Objetivos Presentar una revisión bibliográfica sobre el tema, con énfasis en las implicaciones cardiovasculares de este catión. Fuente de Datos El buscador Pubmed del Servicio Central de Información Biotecnológica de los Institutos Nacionales para la Ciencia, c...

  13. Implicaciones teóricas y empíricas de la nueva definición de desempleo en Colombia.

    OpenAIRE

    Guataquí, Juan; Taborda, Rodrigo

    2005-01-01

    En junio de 2000 Colombia adoptó una nueva definición de desempleo siguiendo los estándares de la OIT. Este cambio implicó una reducción de la tasa de desempleo del orden de dos puntos porcentuales. En este documento contrastamos el caso Colombiano con las experiencias de otros países en términos de la transformación metodológica y sus implicaciones empíricas. Posteriormente, evaluamos el soporte empírico del cambio de metodología. Nuestros resultados sugieren implicaciones esp...

  14. El concepto de materialidad: implicaciones para gerentes e inversionistas

    Directory of Open Access Journals (Sweden)

    Ahmad Hussein Juma’h

    2014-01-01

    Full Text Available Se discuten las implicaciones de la materialidad de la información financiera sobre “manejo de los ingresos”. Implica la descripción y el análisis del contenido de la Codificación de Normas Contables™. Las perspectivas profesionales fueron utilizadas para confirmar la ausencia de directrices de importancia relativa en los Principios de Contabilidad Generalmente Aceptados (en inglés, PCGA de los EE. UU. Los términos “materialidad”, “significancia” e “importancia” fueron usados para determinar la inclusión de materialidad en las codificaciones. Las principales conclusiones indican que, primero, los factores determinantes y las motivaciones internas y externas influyen en las prácticas de “manejos de ganancias” y, segundo, los PCGA de EE. UU. no cuentan con directrices bien definidas para aplicar la materialidad en la toma de decisiones.

  15. Oncolytic Adenoviruses in Cancer Treatment

    Directory of Open Access Journals (Sweden)

    Ramon Alemany

    2014-02-01

    Full Text Available The therapeutic use of viruses against cancer has been revived during the last two decades. Oncolytic viruses replicate and spread inside tumors, amplifying their cytotoxicity and simultaneously reversing the tumor immune suppression. Among different viruses, recombinant adenoviruses designed to replicate selectively in tumor cells have been clinically tested by intratumoral or systemic administration. Limited efficacy has been associated to poor tumor targeting, intratumoral spread, and virocentric immune responses. A deeper understanding of these three barriers will be required to design more effective oncolytic adenoviruses that, alone or combined with chemotherapy or immunotherapy, may become tools for oncologists.

  16. Algunas implicaciones jurídicas del matrimonio entre personas del mismo sexo en la Ciudad de México

    OpenAIRE

    Rojas Martínez, Alejandro

    2011-01-01

    El artículo analiza y expone el reconocimiento del matrimonio entre personas del mismo sexo en la Ciudad de México, así como sus implicaciones primarias a partir de su concreción en el sistema jurídico mexicano. El estudio contiene el análisis de la "Sociedad de Convivencia" como un mecanismo de reconocimiento de derechos de carácter civil a personas con relaciones personales de convivencia permanente, entre las que se encuentran las personas con identidad de género distinta de la correspondi...

  17. Accesibilidad universal: sentido normativo e implicaciones en la educación y la práctica profesional

    Directory of Open Access Journals (Sweden)

    Manuel Aparicio Payá

    2017-06-01

    Full Text Available En la Convención Internacional sobre los Derechos de las Personas con Discapacidad, aprobada por la ONU en 2006, se incluye el derecho a la accesibilidad universal y el diseño para todas las personas. En este trabajo nos ocupamos, en primer lugar, de clarificar el sentido normativo que tiene este derecho. Posteriormente, analizamos sus implicaciones en el ámbito de la ética de las profesiones, mostrando la responsabilidad de los profesionales en el diseño de un espacio social diverso y común. Finalmente, analizamos las implicaciones en la educación universitaria que ha de preparar a los profesionales para realizar tal diseño.

  18. Dramatic Decline of Respiratory Illness Among US Military Recruits After the Renewed Use of Adenovirus Vaccines

    Science.gov (United States)

    2014-10-01

    editors consider relevant to the con- tent of the manuscript have been disclosed. References 1. Broderick MP, Hansen CJ, Russell KL. Exploration of...Russell KL, Broderick MP, Franklin SE, et al. Transmission dynamics and prospective environmental sampling of adenovirus in a military recruit setting

  19. Reflexiones sobre la guerra y sus implicaciones en la salud humana

    Directory of Open Access Journals (Sweden)

    . Luis Angel Pérez Flerima

    2017-11-01

    Full Text Available El presente trabajo está dirigido a la reflexión de un fenómeno complejo y dramático para la vida de millones de seres humanos en el mundo como es la guerra, que con el avance científico- tecnológico ha hecho más terrible su impacto con la correspondiente secuela para la humanidad. Este artículo expresa sucintamente los orígenes de la guerra, el papel que los Estados Unidos desempeñan en los diferentes conflictos regionales como principal potencia imperialista. Por ello se valora el fenómeno de la guerra y sus implicaciones en la salud humana.

  20. Adenovirus sequences required for replication in vivo.

    OpenAIRE

    Wang, K; Pearson, G D

    1985-01-01

    We have studied the in vivo replication properties of plasmids carrying deletion mutations within cloned adenovirus terminal sequences. Deletion mapping located the adenovirus DNA replication origin entirely within the first 67 bp of the adenovirus inverted terminal repeat. This region could be further subdivided into two functional domains: a minimal replication origin and an adjacent auxillary region which boosted the efficiency of replication by more than 100-fold. The minimal origin occup...

  1. 21 CFR 866.3020 - Adenovirus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3020 Adenovirus... identify adenoviruses directly from clinical specimens. The identification aids in the diagnosis of disease...

  2. Desarrollo histórico de la primera ley de la termodinámica y algunas implicaciones didácticas

    OpenAIRE

    Jenny Muñoz Pérez

    2009-01-01

    En el presente ensayo se realiza una breve descripción histórica relacionada con el desarrollo de la primera ley de la termodinámica y su debate entre los principales investigadores de la época, en relación con los conceptos de calor, energía libre y entalpía. Además sus implicaciones didácticas con relación a los errores conceptuales que los estudiantes presentan al momento de abordar esta temática.

  3. Implicaciones teóricas y empíricas de la nueva definición de desempleo en Colombia.

    Directory of Open Access Journals (Sweden)

    Juan Carlos Guataqui

    2010-05-01

    Full Text Available En junio de 2000 Colombia adoptó una nueva definición de desempleo siguiendo los estándares de la OIT. Este cambio implicó una reducción de la tasa de desempleo del orden de dos puntos porcentuales. En este documento contrastamos el caso Colombiano con las experiencias de otros países en términos de la transformación metodológica y sus implicaciones empíricas. Posteriormente, evaluamos el soporte empírico del cambio de metodología. Nuestros resultados sugieren implicaciones específicas sobre el desempleo estructural y su relación con el perfil educativo de los desempleados; por lo tanto, cuestionan la aplicación práctica de la definición estándar de desempleo de la OIT al caso colombiano.

  4. Implicaciones sociales de la ciencia y la tecnología movilizadas en investigaciones STSPs. La visión del alumnado y del profesorado

    OpenAIRE

    Lupión Cobos, Teresa; Franco-Mariscal, Antonio Joaquín

    2017-01-01

    Se muestran los resultados de un estudio con profesores y estudiantes de secundaria que participaron en investigaciones que implican un modelo colaborativo estudiante-profesor-científico (STPSs) en el marco Science IES, referido a las implicaciones sociales de la ciencia y la tecnología movilizadas por estas investigaciones. Como instrumento se usaron varias cuestiones del cuestionario TOSRA sobre actitudes hacia la ciencia. Los resultados evidencian avances importantes en el cambio de actitu...

  5. Estudio del significado de las malformaciones congénitas y sus implicaciones en los programas de salud

    OpenAIRE

    Struck, Adelaida; Struck Vidal -Aguilera, Mony  de Lourdes; Chávez, Yohanna; Fernández, Francisco J

    2008-01-01

    En el siguiente artículo se realiza un estudio acerca del significado de las malformaciones congénitas y sus implicaciones en los programas de prevención para la población venezolana. Se hace uso de metodologías cualitativas y cuantitativas, utilizando como técnica para la recolección de los datos, el cuestionario. Su aplicación se lleva a cabo en dos centros maternos, Maternidad Concepción Palacios y Centro Materno Leopoldo Aguerrevere en Caracas-Venezuela. Se analizan como variables: el con...

  6. Los Residuos Sólidos en el Sur de Tamaulipas y sus implicaciones socioeconómicas

    OpenAIRE

    Treviño Hernández, Raúl

    2017-01-01

    La presente investigación aborda, desde la perspectiva de la planeación territorial, uno de los principales problemas de las ciudades, como es la Gestión Integral de los Residuos Sólidos Urbanos (RSU) y sus implicaciones socioeconómicas; esto es debido al constante crecimiento de la población y al continuo cambio en los patrones de consumo. Este trabajo se organiza en una presentación de la investigación y cuatro partes: La presentación se inicia con una introducción donde se aborda de ...

  7. Cancer gene therapy with targeted adenoviruses.

    Science.gov (United States)

    Bachtarzi, Houria; Stevenson, Mark; Fisher, Kerry

    2008-11-01

    Clinical experience with adenovirus vectors has highlighted the need for improved delivery and targeting. This manuscript aims to provide an overview of the techniques currently under development for improving adenovirus delivery to malignant cells in vivo. Primary research articles reporting improvements in adenoviral gene delivery are described. Strategies include genetic modification of viral coat proteins, non-genetic modifications including polymer encapsulation approaches and pharmacological interventions. Reprogramming adenovirus tropism in vitro has been convincingly demonstrated using a range of genetic and physical strategies. These studies have provided new insights into our understanding of virology and the field is progressing. However, there are still some limitations that need special consideration before adenovirus-targeted cancer gene therapy emerges as a routine treatment in the clinical setting.

  8. Deaths from Adenovirus in the US Military

    Centers for Disease Control (CDC) Podcasts

    Dr. Joel Gaydos, science advisor for the Armed Forces Health Surveillance Center, and Dr. Robert Potter, a research associate for the Armed Forces Medical Examiner System, discuss deaths from adenovirus in the US military.

  9. Adenovirus-vectored Ebola vaccines.

    Science.gov (United States)

    Gilbert, Sarah C

    2015-01-01

    The 2014 outbreak of Ebola virus disease in West Africa has highlighted the need for the availability of effective vaccines against outbreak pathogens that are suitable for use in frontline workers who risk their own health in the course of caring for those with the disease, and also for members of the community in the affected area. Along with effective contact tracing and quarantine, use of a vaccine as soon as an outbreak is identified could greatly facilitate rapid control and prevent the outbreak from spreading. This review describes the progress that has been made in producing and testing adenovirus-based Ebola vaccines in both pre-clinical and clinical studies, and considers the likely future use of these vaccines.

  10. Core labeling of adenovirus with EGFP

    International Nuclear Information System (INIS)

    Le, Long P.; Le, Helen N.; Nelson, Amy R.; Matthews, David A.; Yamamoto, Masato; Curiel, David T.

    2006-01-01

    The study of adenovirus could greatly benefit from diverse methods of virus detection. Recently, it has been demonstrated that carboxy-terminal EGFP fusions of adenovirus core proteins Mu, V, and VII properly localize to the nucleus and display novel function in the cell. Based on these observations, we hypothesized that the core proteins may serve as targets for labeling the adenovirus core with fluorescent proteins. To this end, we constructed various chimeric expression vectors with fusion core genes (Mu-EGFP, V-EGFP, preVII-EGFP, and matVII-EGFP) while maintaining expression of the native proteins. Expression of the fusion core proteins was suboptimal using E1 expression vectors with both conventional CMV and modified (with adenovirus tripartite leader sequence) CMV5 promoters, resulting in non-labeled viral particles. However, robust expression equivalent to the native protein was observed when the fusion genes were placed in the deleted E3 region. The efficient Ad-wt-E3-V-EGFP and Ad-wt-E3-preVII-EGFP expression vectors were labeled allowing visualization of purified virus and tracking of the viral core during early infection. The vectors maintained their viral function, including viral DNA replication, viral DNA encapsidation, cytopathic effect, and thermostability. Core labeling offers a means to track the adenovirus core in vector targeting studies as well as basic adenovirus virology

  11. La comprensión de las organizaciones empresariales y su ambiente como sistemas de complejidad creciente: rasgos e implicaciones

    Directory of Open Access Journals (Sweden)

    Luz Esperanza Bohórquez

    2016-10-01

    Método: en el documento se revisan las características de los sistemas de complejidad creciente y se identifican los elementos que explican su capacidad de auto-organización. A partir de esta revisión se plantean las implicaciones de comprender a las organizaciones como sistemas de complejidad creciente, las cuales se articulan con recientes investigaciones que en ingeniería organizacional y gestión buscan incrementar la capacidad de adaptación del sistema. Resultados: se desarrolla una apreciación sobre las implicaciones en la ingeniería organizacional de diseñar organizaciones empresariales con mayor capacidad para auto-organizarse y de manera amplia de aprovechar la complejidad creciente del entorno. Conclusiones: la comprensión de las organizaciones empresariales como sistemas de complejidad creciente ofrece una perspectiva notoriamente distinta al paradigma tradicional el cual se han caracterizado por el enfoque reduccionista. A la fecha la investigación al respecto se encuentra en etapas tempranas y no existen modelos o evidencias empíricas que permitan corroborar de manera amplia y completa la fuerza de tales ideas. Sin embargo, los altos niveles de agilidad, flexibilidad y robustez que exhiben los sistemas auto-organizados permiten proyectar importantes campos de investigación alrededor del diseño de estructuras organizacionales flexibles, formas de gobernanza, toma decisiones en ausencia de controlador central, etc.

  12. Web 3.0: implicaciones educativas

    OpenAIRE

    Grupo TACE. Tecnologías Aplicadas a las Ciencias de la Educación

    2012-01-01

    La Web 3.0 se considera la etapa que sigue a la Web 2.0 o Web social. Todavía no es un término unívoco y suele aparecer unido con la web semántica. Es una extensión del World Wide Web, que permite expresar el lenguaje natural y también utilizar un lenguaje que se puede entender, interpretar y utilizar por agentes software, permitiendo encontrar, compartir e integrar la información más fácilmente. Se trata de un nuevo ciclo en el que la inteligencia artificial se combina con la capacidad de l...

  13. Construction and evaluation of novel rhesus monkey adenovirus vaccine vectors.

    Science.gov (United States)

    Abbink, Peter; Maxfield, Lori F; Ng'ang'a, David; Borducchi, Erica N; Iampietro, M Justin; Bricault, Christine A; Teigler, Jeffrey E; Blackmore, Stephen; Parenteau, Lily; Wagh, Kshitij; Handley, Scott A; Zhao, Guoyan; Virgin, Herbert W; Korber, Bette; Barouch, Dan H

    2015-02-01

    Adenovirus vectors are widely used as vaccine candidates for a variety of pathogens, including HIV-1. To date, human and chimpanzee adenoviruses have been explored in detail as vaccine vectors. The phylogeny of human and chimpanzee adenoviruses is overlapping, and preexisting humoral and cellular immunity to both are exhibited in human populations worldwide. More distantly related adenoviruses may therefore offer advantages as vaccine vectors. Here we describe the primary isolation and vectorization of three novel adenoviruses from rhesus monkeys. The seroprevalence of these novel rhesus monkey adenovirus vectors was extremely low in sub-Saharan Africa human populations, and these vectors proved to have immunogenicity comparable to that of human and chimpanzee adenovirus vaccine vectors in mice. These rhesus monkey adenoviruses phylogenetically clustered with the poorly described adenovirus species G and robustly stimulated innate immune responses. These novel adenoviruses represent a new class of candidate vaccine vectors. Although there have been substantial efforts in the development of vaccine vectors from human and chimpanzee adenoviruses, far less is known about rhesus monkey adenoviruses. In this report, we describe the isolation and vectorization of three novel rhesus monkey adenoviruses. These vectors exhibit virologic and immunologic characteristics that make them attractive as potential candidate vaccine vectors for both HIV-1 and other pathogens. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  14. Deficiencia de zinc y sus implicaciones funcionales

    Directory of Open Access Journals (Sweden)

    ROSADO JORGE L

    1998-01-01

    Full Text Available El presente trabajo tiene por objeto revisar los aspectos teóricos y los estudios realizados en México que sugieren la existencia de la deficiencia moderada de zinc en niños de población rural, así como algunas de las consecuencias de dicha deficiencia en la salud. El zinc es un nutrimento indispensable para el organismo de los humanos y juega un papel importante en una serie de procesos metabólicos: participa en el sitio catalítico de varios sistemas enzimáticos; participa como ion estructural en membranas biológicas, y guarda una estrecha relación con la síntesis de proteínas, entre otras cosas. Es por esto que la deficiencia de zinc está asociada con consecuencias importantes en la salud y la funcionalidad de los individuos, especialmente durante las primeras etapas de la vida. De relevancia para México es la existencia de una deficiencia moderada de zinc en los niños y las consecuencias que ésta pueda tener en la salud de los mismos. Los estudios realizados sugieren que la deficiencia moderada de zinc se presenta asociada con la ingestión de dietas basadas en alimentos de origen vegetal, las cuales contienen cantidades importantes de inhibidores de la absorción de zinc. Este tipo de dietas se consume habitualmente en las zonas rurales y en la población marginal de las ciudades en el país. Entre las consecuencias más importantes de esta deficiencia se encontró un aumento en la presencia de enfermedades infecciosas, especialmente de diarrea, y posibles alteraciones en el desarrollo de la capacidad cognoscitiva.

  15. Prevalencia de la infección con el virus linfotrópico de células T humanas de tipo 1 y 2 en donantes de sangre en Colombia, 2001-2014: implicaciones sobre la seguridad de la transfusión

    Directory of Open Access Journals (Sweden)

    María Isabel Bermúdez-Forero

    2016-08-01

    Conclusiones. Los resultados obtenidos sugieren que la infección por HTLV 1 y 2 se distribuye en varias zonas del país que no eran catalogadas como endémicas. Se ratificó la importancia de la tamización universal de las unidades de sangre captadas, para minimizar el riesgo de infección con este agente por la vía de la transfusión.

  16. Components of Adenovirus Genome Packaging

    Science.gov (United States)

    Ahi, Yadvinder S.; Mittal, Suresh K.

    2016-01-01

    Adenoviruses (AdVs) are icosahedral viruses with double-stranded DNA (dsDNA) genomes. Genome packaging in AdV is thought to be similar to that seen in dsDNA containing icosahedral bacteriophages and herpesviruses. Specific recognition of the AdV genome is mediated by a packaging domain located close to the left end of the viral genome and is mediated by the viral packaging machinery. Our understanding of the role of various components of the viral packaging machinery in AdV genome packaging has greatly advanced in recent years. Characterization of empty capsids assembled in the absence of one or more components involved in packaging, identification of the unique vertex, and demonstration of the role of IVa2, the putative packaging ATPase, in genome packaging have provided compelling evidence that AdVs follow a sequential assembly pathway. This review provides a detailed discussion on the functions of the various viral and cellular factors involved in AdV genome packaging. We conclude by briefly discussing the roles of the empty capsids, assembly intermediates, scaffolding proteins, portal vertex and DNA encapsidating enzymes in AdV assembly and packaging. PMID:27721809

  17. Efectos musculares y hematológicos del uso de rHuEpo en combinación con el entrenamiento físico. Implicaciones de la demospresina y la hipoxia en el dopaje sanguineo. Estudios in vivo e in vitro.

    OpenAIRE

    Martínez Bello, Vladimir Essau

    2011-01-01

    En la presente Tesis Doctoral hemos querido estudiar, en primer lugar, el efecto que tendría el incremento artificial de la capacidad de transporte de oxígeno, a través del tratamiento con rHuEpo, sobre distintos parámetros musculares así como su implicación en la mejora del rendimiento físico. Son muchos los autores que, tanto en el ámbito clínico (Winearls, Oliver y cols. 1986; Bommer, Muller-Buhl y cols. 1987) como en el ámbito deportivo (Berglund y Ekblom 1991; Lundby, Robach y cols. 2008...

  18. burnout y estrategias de inteligencia emocional en profesores universitarios: implicaciones en la salud laboral educativa

    Directory of Open Access Journals (Sweden)

    Betsy Ilaja

    2016-01-01

    Full Text Available Diversos estudios sitúan a la docencia universitaria como una de las profesiones con mayor riesgo a experimentar inestabilidad y estrés laboral. La presente investigación exploró el ni- vel de burnout y las habilidades en inteligencia emocional en una muestra de 60 profesores universitarios de tiempo completo de la Pontificia Universidad Católica del Ecuador, sede Ambato. Se realizaron análisis de mediación múltiple y se encontró que: a las tareas de in- vestigación y vinculación se asocian significativamente a burnout, y la tarea de docencia es percibida como desempeño óptimo, b la salud actúa como variable mediadora entre estrés laboral y burnout, y el género solo se relaciona con burnout, y c la regulación emocional media la relación entre desempeño docente y satisfacción laboral. Se discuten las implicaciones de estos hallazgos en la salud laboral de docentes universitarios.

  19. Inclusão social da pessoa com deficiência: conquistas, desafios e implicações para a enfermagem La inclusión social de la persona con deficiencia: conquistas, desafíos y implicaciones para la enfermería Social inclusion of disable people: achievements, challenges and implications for the nursing area

    Directory of Open Access Journals (Sweden)

    Inacia Sátiro Xavier de França

    2009-03-01

    Full Text Available Objetivou-se compreender o discurso das pessoas com deficiência acerca da concretude do processo de inclusão social e articular o discurso desses sujeitos com as diretrizes do Decreto nº 3.298/99. Foram entrevistadas seis pessoas com deficiência física, seis auditivos e seis visuais Os dados foram padronizados e categorizados em dois núcleos de sentidos: diretrizes pré-inclusivistas da pessoa com deficiência, e diretrizes inclusivistas da pessoa com deficiência. A perspectiva metodológica foi a análise de discurso. Conclui-se que esses sujeitos conquistaram legislação que assegura direitos de cidadania, mas existem desafios no campo da saúde, educação, profissionalização e da inserção no mercado de trabalho. Os enfermeiros reabilitadores precisam conhecer a vivência desses sujeitos e dos seus familiares para ajudá-los a buscar estratégias de enfrentamento dos problemas que afetam a sua inclusão e, conseqüentemente, a sua saúde.El objetivo de la investigación fue comprender el discurso de las personas con deficiencia acerca de cuán efectivo es el proceso de inclusión social y articular el discurso de eses sujetos con las directrices del Decreto nº 3.298/99. Fueron entrevistadas seis personas con deficiencia física, seis auditivos y seis visuales Los datos fueron estandarizados y clasificados en categorías en dos núcleos de sentidos: directrices pre-inclusivistas de la persona con deficiencia, y directrices inclusivistas de la persona con deficiencia. La perspectiva metodológica fue el análisis de discurso. Se concluye que eses sujetos conquistaron una legislación que asegura derechos de ciudadanía, sin embargo existen desafíos en el campo de la salud, educación, profesionalización y de la inserción en el mercado de trabajo. Los enfermeros que actúan en rehabilitación necesitan conocer la vivencia de eses sujetos y de sus familiares para ayudarlos a buscar estrategias de enfrentamiento de los problemas

  20. Prevalência de Hipertensão Arterial em pessoas com mobilidade física prejudicada: implicações para a enfermagem Prevalencia de Pressión Alta en personas con movilidad física afectada: implicaciones para la enfermería Prevalence of High Blood Pressure in people with impaired physical mobility: nursing implications

    Directory of Open Access Journals (Sweden)

    Jeová Alves de Souza

    2008-12-01

    Full Text Available A hipertensão arterial é uma das principais causas de morte em todo o mundo, e pode resultar da redução ou ausência do tônus muscular esquelético. Objetivou-se identificar aspectos epidemiológicos da hipertensão arterial em pessoas com deficiência física e o conhecimento desses indivíduos acerca do controle dos fatores de risco para hipertensão arterial. Participaram 40 pessoas com deficiência física. Utilizou-se um questionário abordando fatores de risco para hipertensão arterial. Os dados foram analisados à luz de multireferenciais. Detectou-se 30% com sobrepeso ou obesidade; 75% com PA inferior ou igual a 120/80 mmHg; 25% hipertensos e 20% hipertensos de estágio I. Os sujeitos desconhecem os riscos para hipertensão. Seus hábitos e estilo de vida oferecem riscos à saúde. Conclue-se que a hipertensão em pessoas com deficiência física alcança maior gravidade e representa um desafio aos profissionais de saúde, pois o controle desse agravo imprescinde da adesão do paciente ao tratamento.La hipertensión arterial es una de las principales causas de muerte en todo el mundo y puede resultar de la reducción o ausencia del tonos muscular esquelético. Se ha objetivado identificar aspectos epidemiológicos de la hipertensión arterial en personas con deficiencia física y el conocimiento de esos individuos acerca del control de los factores de riesgo para la hipertensión arterial. Participaron 40 personas con ficiencia física. Se utilizo un cuestionario abordando factores de riesgo para hipertensión arterial. Los datos fueron analizados bajo la orientación de multireferenciales. Se detecto 30% con peso elevado o obesidad; 75% con PA inferior o igual a 120/80 mmHg; 25% hipertensos y 20% hipertensos de nivel 1. Los sujetos desconocen los riesgos para hipertensión. Sus hábitos y estilo de vida ofrecen riesgos a la salud. La hipertensión en personas con deficiencia física alcanza mayor gravedad y representa un desaf

  1. Circumvention of Immunity to the Adenovirus Major Coat Protein Hexon

    Science.gov (United States)

    Roy, Soumitra; Shirley, Pamela S.; McClelland, Alan; Kaleko, Michael

    1998-01-01

    Immunity to adenoviruses is an important hurdle to be overcome for successful gene therapy. The presence of antibodies to the capsid proteins prevents efficacious adenovirus vector administration in vivo. We tested whether immunity to a particular serotype of adenovirus (Ad5) may be overcome with a vector that encodes the hexon sequences from a different adenovirus serotype (Ad12). We successfully constructed an adenovirus vector with a chimeric Ad5-Ad12 hexon which was not neutralized by plasma from C57BL/6 mice immunized with Ad5. The vector was also capable of transducing the livers of C57BL/6 mice previously immunized with Ad5. PMID:9658137

  2. Teorias curriculares, perspectivas teóricas em Educação Física Escolar e implicações para a formação docente Teorías curriculares, perspectivas teóricas en educación física y las implicaciones con la formación docente Curriculum theories, theoretical perspectives in physical education and implications for school teacher training

    Directory of Open Access Journals (Sweden)

    Osvaldo Luiz Ferraz

    2012-09-01

    Full Text Available A função do professor de educação física escolar pode ser definida pela elaboração, implantação e avaliação de programas que tematizam, do ponto de vista didático-pedagógico, jogos, esportes, lutas, ginásticas, danças, exercícios físicos, entre outros; com a intenção de influenciar a formação dos alunos para a participação democrática na vida em sociedade. Todavia, este consenso se dilui quando aplicado ao campo do currículo e da formação docente. No campo do currículo, apesar do reconhecimento de que a tematização didático-pedagógica se faz a partir de sentidos culturais e das potencialidades de estimulação do organismo humano que se apresentam nas manifestações da cultura de movimento; a intencionalidade, a organização curricular e a sistematização do conhecimento podem divergir consideravelmente modificando o papel do componente curricular na educação básica. Sendo assim, este texto analisa aspectos do desenvolvimento das teorias curriculares, relacionando-as às perspectivas teóricas em Educação Física. Em seguida, implicações deste debate são relacionadas à formação docente.La función del profesor de educación física escolar puede ser definida por la elaboración, implantación y evaluación de programas que tematizan, desde el punto de vista didáctico-pedagógico, juegos, deportes, luchas, gimnasia, danzas, ejercicios físicos, entre otros; con la intención de influenciar la formación de los alumnos para la participación democrática de la vida en sociedad. Todavía, este consenso se diluye cuando es aplicado al campo del currículo y de la formación docente. En el campo del currículo, a pesar del reconocimiento de que la tematización didáctico-pedagógica se hace a partir de los sentidos culturales y de las potencialidades de estimulación del organismo humano que se presentan en las manifestaciones de la cultura del movimiento; la intencionalidad, la organización curricular

  3. Implicaciones del VIH/SIDA en la biografía de varones con prácticas homoeróticas en la Ciudad de México Implications of AIDS on the biography of men with homoerotics practices in Mexico City

    Directory of Open Access Journals (Sweden)

    Gabriel Gallego-Montes

    2010-04-01

    Full Text Available OBJETIVO: Determinar los cambios en la configuración biográfica de un conjunto de varones con prácticas homoeróticas en la era del sida. MATERIAL Y MÉTODOS: Se aplicó una encuesta retrospectiva durante el primer semestre de 2006 a una muestra intencional de 250 varones en la Ciudad de México. Con ellos fue posible reconstruir 633 historias de pareja ocurridas en diferentes momentos del tiempo. Por otro lado, tomando como base el comportamiento epidemiológico del VIH/SIDA en México, se construyeron tres cohortes de emparejamiento: "antes del sida" 1970-1984; 1985-1995 o periodo de "crecimiento exponencial" y 1996-2005 o de "estabilización" de la epidemia. RESULTADOS: Durante el periodo en estudio se encontró una menor exclusividad sexual y una menor duración en las relaciones de pareja, entre cohortes de emparejamientos. Este proceso ha sido documentado en otros lugares y se le ha llamado de "distensión sexual". CONCLUSIONES: El estudio aporta elementos para una historia social de la epidemia y el mejoramiento de políticas públicas de prevención en torno al VIH/SIDA.OBJECTIVE: To determine the changes in the biography of men with homoerotic practices in the times of AIDS. MATERIAL AND METHODS: A retrospective (biographical survey was administered during the first semester of 2006 to 250 gay/homosexual men in Mexico City. Based on this survey, 633 couple stories, taking place in different time periods, were reconstructed. In addition, taking into account the epidemiologic behavior of HIV-AIDS in Mexico, three couple cohorts were defined as: "before AIDS" 1970-1984; period of "exponential growth" 1985-1995; and "stabilization period" 1996-2005. The 633 stories are distributed along these time-frames, taking the starting date of each relationship as the criterion. RESULTS: During the period of this study, less sexual exclusivity and shorter duration of sexual relationships were found among couple cohorts. The latter process has been

  4. Mouse adenovirus type 1 infection of macrophages

    NARCIS (Netherlands)

    Ashley, S.L.; Welton, A.R.; Harwood, K.M.; Rooijen, van N.; Spindler, K.R.

    2009-01-01

    Mouse adenovirus type 1 (MAV-1) causes acute and persistent infections in mice, with high levels of virus found in the brain, spinal cord and spleen in acute infections. MAV-1 infects endothelial cells throughout the mouse, and monocytes/macrophages have also been implicated as targets of the virus.

  5. Deaths from Adenovirus in the US Military

    Centers for Disease Control (CDC) Podcasts

    2012-03-26

    Dr. Joel Gaydos, science advisor for the Armed Forces Health Surveillance Center, and Dr. Robert Potter, a research associate for the Armed Forces Medical Examiner System, discuss deaths from adenovirus in the US military.  Created: 3/26/2012 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 3/29/2012.

  6. El cigarrillo: implicaciones para la salud

    Directory of Open Access Journals (Sweden)

    Manuel Antonio Ballén

    2006-07-01

    Full Text Available Antecedentes. El consumo de cigarrillo, según cálculos de la Organización Mundial de la Salud (OMS, es la causa de por lo menos cuatro millones de muertes al año. Las consecuencias de fumar cigarrillo van desde cambios fisiopatológicos en los sistemas respiratorio, cardiovascular y digestivo, hasta trastornos mentales asociados a la dependencia a la nicotina. Objetivo. Realizar una revisión narrativa de la literatura médica mostrando los efectos del consumo de cigarrillo sobre la salud física y mental en los fumadores activos y pasivos. Material y métodos. El artículo se basa en la revisión de artículos a través de la base de datos del MEDLINE y de la biblioteca Virtual de la OMS. Se emplearon en la búsqueda las palabras clave "Cigarette Smoke", "Cancer AND smoke", "COPD AND smoke", "Nicotine Dependence". Se escogieron artículos y libros publicados en idioma inglés entre los años 1994 y 2006, realizando una lectura crítica (análisis de posibles conflictos de interés y errores de diseño. Conclusión. El humo del cigarrillo contiene partículas potencialmente peligrosas para la salud de quien está expuesto a ellas. De este modo, fumar cigarrillo se convierte en un factor etiológico común a muchos tipos de cáncer. Además los componentes del cigarrillo están relacionados con el desarrollo de otros estados patológicos (enfermedad cardiovascular y enfermedad pulmonar obstructiva crónica. La nicotina, uno de sus componentes, es un potente agente adictivo. Todo esto en su conjunto hace del cigarrillo un importante problema de salud pública.

  7. Chimpanzee Adenovirus Vector Ebola Vaccine.

    Science.gov (United States)

    Ledgerwood, Julie E; DeZure, Adam D; Stanley, Daphne A; Coates, Emily E; Novik, Laura; Enama, Mary E; Berkowitz, Nina M; Hu, Zonghui; Joshi, Gyan; Ploquin, Aurélie; Sitar, Sandra; Gordon, Ingelise J; Plummer, Sarah A; Holman, LaSonji A; Hendel, Cynthia S; Yamshchikov, Galina; Roman, Francois; Nicosia, Alfredo; Colloca, Stefano; Cortese, Riccardo; Bailer, Robert T; Schwartz, Richard M; Roederer, Mario; Mascola, John R; Koup, Richard A; Sullivan, Nancy J; Graham, Barney S

    2017-03-09

    The unprecedented 2014 epidemic of Ebola virus disease (EVD) prompted an international response to accelerate the availability of a preventive vaccine. A replication-defective recombinant chimpanzee adenovirus type 3-vectored ebolavirus vaccine (cAd3-EBO), encoding the glycoprotein from Zaire and Sudan species, that offers protection in the nonhuman primate model, was rapidly advanced into phase 1 clinical evaluation. We conducted a phase 1, dose-escalation, open-label trial of cAd3-EBO. Twenty healthy adults, in sequentially enrolled groups of 10 each, received vaccination intramuscularly in doses of 2×10 10 particle units or 2×10 11 particle units. Primary and secondary end points related to safety and immunogenicity were assessed throughout the first 8 weeks after vaccination; in addition, longer-term vaccine durability was assessed at 48 weeks after vaccination. In this small study, no safety concerns were identified; however, transient fever developed within 1 day after vaccination in two participants who had received the 2×10 11 particle-unit dose. Glycoprotein-specific antibodies were induced in all 20 participants; the titers were of greater magnitude in the group that received the 2×10 11 particle-unit dose than in the group that received the 2×10 10 particle-unit dose (geometric mean titer against the Zaire antigen at week 4, 2037 vs. 331; P=0.001). Glycoprotein-specific T-cell responses were more frequent among those who received the 2×10 11 particle-unit dose than among those who received the 2×10 10 particle-unit dose, with a CD4 response in 10 of 10 participants versus 3 of 10 participants (P=0.004) and a CD8 response in 7 of 10 participants versus 2 of 10 participants (P=0.07) at week 4. Assessment of the durability of the antibody response showed that titers remained high at week 48, with the highest titers in those who received the 2×10 11 particle-unit dose. Reactogenicity and immune responses to cAd3-EBO vaccine were dose-dependent. At

  8. Sobre las implicaciones normativas de la neurociencia social

    OpenAIRE

    Salles, Arleen

    2013-01-01

    En las últimas décadas, la neurociencia y la psicología cognitiva nos ha permitido comenzar a entender la relación entre procesos psicológicos diversos y hasta sobre las bases neurológicas de las creencias, las emociones, los juicios y las decisiones morales. Pese a la aceptada distinción entre el carácter descriptivo de la ciencia y el normativo de la moralidad, algunos autores afirman que los resultados de los estudios neurocientíficos tienen implicaciones normativas. No es c...

  9. Family network of children with special health needs: implications for Nursing La red familiar de niños con necesidades especiales de salud: implicaciones para la enfermería Rede familial de crianças com necessidades especiais de saúde: implicações para a enfermagem

    Directory of Open Access Journals (Sweden)

    Eliane Tatsch Neves

    2013-04-01

    Full Text Available OBJECTIVE: to describe appropriate sources and resources for caregivers of children with special health needs in the community. METHOD: A qualitative study that used the creativity and sensitivity dynamics speaking map, part of the sensitive creative method, involving 11 caregivers of children with special health needs who are assisted in a university hospital located in the South of Brazil. RESULTS: the maps graphically represented through the genogram and ecomap showed that the caregiving women consistently and regularly use the resources of the internal and external family network; they eventually and irregularly access the community social network for physical and psychological support. CONCLUSION: the reclusive nature of care for these children inside the family circle contributes to their social invisibility. Based on this new information, it is recommended that Nursing participate in the care that is focused on these children's families, with particular attention to their socio-cultural conditions. OBJETIVO: describir fuentes y recursos apropiados por las cuidadoras de niños con necesidades especiales de salud en la comunidad. MÉTODO: estudio cualitativo que utilizó la dinámica de creatividad y sensibilidad mapa hablante del método creativo sensible junto a 11 cuidadoras de niños con necesidades especiales de salud atendidas en un hospital de enseñanza de la región sur de Brasil. RESULTADOS: los mapas gráficamente representados en la forma de genograma y ecomapa revelaron que las mujeres cuidadoras se apropian de forma consistente y regular de los recursos de la red familiar interna y externa; de modo eventual e irregular, acceden a la red social de la comunidad para apoyarles física y espiritualmente. CONCLUSIÓN: el carácter aislado del cuidado a esos niños en el interior de la familia contribuye para su invisibilidad social. A partir de ese conocimiento nuevo, se recomienda que la enfermería se haga presente en el cuidado

  10. Segregación socioespacial urbana. Debates contemporáneos e implicaciones para las ciudades mexicanas

    Directory of Open Access Journals (Sweden)

    Enrique Pérez-Campuzano

    2011-01-01

    Full Text Available La segregación socioespacial es un tema recurrente en disciplinas como la sociología y la geografia; sin embargo ha sido poco tratado en la literatura especializada en lWéxico, quizás por su falta de anclaje teó¡ico claro o por la escasez de datos específicos para medirla. En este trabajo se presenta una aproximación a la temática con especial atención en la construcción teórica y empírica en los países desarrollados y en las posibles implicaciones para los estudios sobre ciudades mexicanas.

  11. Implicaciones de la teoría motivacional de la Autodeterminación en el ámbito laboral

    Directory of Open Access Journals (Sweden)

    Juan Alberto Vargas Téllez

    2012-01-01

    Full Text Available Dada su importancia y pertinencia para la motivación en el trabajo, se aborda la Teoría de la Autodeterminación (TAD en relación con el ámbito laboral. Se argumenta sobre las implicaciones positivas que puede tener para esclarecer algunas controversias que generaron los con ceptos de motivación extrínseca e intrínseca, así como para abordar ciertos temas fundamentales de la organización contemporánea: los sistemas de incentivos, la cultura organizacional, el aprendizaje organizacional y la gestión del conocimiento; se concluy e que estos procesos organizacionales contribuirán más a la productividad en la medida que sean congruentes con un cultura laboral que favorezca la satisfacción de tres necesidades psicológicas básicas relacionadas con el trabajo: la autonomía, la competen cia y la socialización o relación, conceptos que postula la Teoría de la Autodeterminación.

  12. Algunas implicaciones jurídicas del matrimonio entre personas del mismo sexo en la Ciudad de México

    Directory of Open Access Journals (Sweden)

    Alejandro Rosas Martínez

    2011-07-01

    Full Text Available El artículo analiza y expone el reconocimiento del matrimonio entre personas del mismo sexo en la Ciudad de México, así como sus implicaciones primarias a partir de su concreción en el sistema jurídico mexicano. El estudio contiene el análisis de la “Sociedad de Convivencia” como un mecanismo de reconocimiento de derechos de carácter civil a personas con relaciones personales de convivencia permanente, entre las que se encuentran las personas con identidad de género distinta de la correspondiente a su sexo y las personas con preferencia sexual distinta de la heterosexual. También se estudia la reforma por la que el matrimonio entre personas del mismo sexo es posible y el criterio que la Suprema Corte de Justicia de México emitió sobre aquélla con base en una acción de inconstitucionalidad en la que se planteó el alcance del principio de igualdad en el constitucionalismo mexicano a partir de los conceptos de familia, matrimonio, heterosexualidad, homosexualidad, adopción e interés superior del menor.

  13. Rasgos psicopáticos infanto-juveniles: evaluación e implicaciones en un estudio prospectivo

    Directory of Open Access Journals (Sweden)

    Estrella Romero

    2016-01-01

    Full Text Available En este trabajo se analizan los datos de un estudio longitudinal a largo plazo con el objetivo de examinar las implicaciones de los rasgos psicopáticos infanto-juveniles, evaluados a través de la mCPS (modified Child Psychopathy Scale, en la conducta antisocial en la adolescencia. Se analizan los datos recogidos en 141 niños (edad media = 7.80, que fueron seguidos seis y diez años más tarde. Los resultados muestran que los niños con altas puntuaciones en rasgos psicopáticos presentan en la adolescencia temprana niveles relativamente altos de problemas de conducta. Además, a lo largo de la adolescencia se produce en ellos un mayor incremento en implicación con amigos antisociales, consumo de cannabis y agresión proactiva. Así, los resultados muestran que los rasgos psicopáticos medidos en la niñez se asocian con un desarrollo más desfavorable, particularmente en indicadores que, como la agresión proactiva, se han vinculado clásicamente a la personalidad psicopática en adultos.

  14. TRAUMA OCULAR CERRADO: MANIFESTACIONES, MANEJO E IMPLICACIONES EN EL BOXEO Y EL FÚTBOL

    Directory of Open Access Journals (Sweden)

    Álvaro Rodríguez González

    2014-12-01

    Full Text Available El trauma ocular cerrado según su grado puede ser ligero moderado o severo. En algunos casos permanece inadvertido o enmascarado y crea consecuencias por lo que debe ser investigado. El trauma tiene características y deja huellas, algunas evidentes y otras ocultas que pueden aparecer en la historia clínica o deben ser buscadas en el segmento anterior ocular en donde se pueden observar trastornos del reflejo pupilar, (incluye midriasis paralítica, laceraciones palpebrales, hemorragia orbitaria, hifema (hemorragia en cámara anterior y fracturas óseas, orbitaria o maxilofacial. En biomicroscopía, pueden observarse laceraciones corneanas, a veces sutiles, otras veces son cicatrices perforantes enmascaradas, acompañadas interiormente de sinequia anterior del iris a la cicatriz de la córnea. También, heridas corneanas recientes, diálisis del iris, a veces extensas en su base, con retroinserción angular de variable extensión y tunelización supracoroidea que inducen, bien a hipotonía ocular o a elevación de la tensión ocular y establecimiento de glaucomas. El trauma ocular cerrado es importante por haberse convertido en un problema de salud pública dadas sus implicaciones en niños, en trabajadores y en aficionados y profesionales en los deportes.

  15. Implicaciones de la securitización en América desde la particularidad Colombiana

    Directory of Open Access Journals (Sweden)

    Nathaly Jiménez Reynales

    2004-01-01

    Full Text Available Este artículo da cuenta de las implicaciones que surgen a partir de un análisis constructivista de la relación constitutiva entre los temas de agenda de la política exterior de un país como Colombia y el resultado de su integración en la región. Ello explica que mientras exista un condicionante como la securitización en la política de un país será difícil concertar políticas comunes con otras naciones. El caso de las drogas ilícitas, por ejemplo, le ha generado al Estado colombiano problemas de coordinación entre la política antidrogas que adelanta y su capacidad de maniobra en un escenario específico como el andino. Igualmente, la actual política de defensa y seguridad democrática manifiesta una ruptura entre lo que se entiende como seguridad democrática y la percepción que la sociedad tiene de ella. Ambos casos ayudarán a comprender cómo al securitizar un asunto político el Estado puede perder su dirección tanto en el ámbito internacional como en el doméstico.

  16. Seguridad en el mar, algunas implicaciones de los códigos IGS y PBIB.

    Directory of Open Access Journals (Sweden)

    José Vicente Guzmán Escobar

    2007-12-01

    Full Text Available La expedición del Código Internacional de Gestión de la Seguridad Marítima (Código IGS y del Código de Protección de Buques e Instalaciones Portuarias (Código PBIB, en el contexto del Convenio SOLAS, tiene especiales implicaciones legales en diferentes figuras del derecho marítimo internacional, tales como la limitación de responsabilidad de los armadores de buques por reclamaciones marítimas, la limitación de responsabilidad de los armadores por contaminación por derrames de hidrocarburos, la limitación de responsabilidad del transportador marítimo de mercancías, la obligación del armador y del transportador marítimo en relación con la navegabilidad del buque, los contratos de fletamento y las coberturas de los Clubes de Protección e Indemnización. El presente artículo hace una descripción de las posibles consecuencias jurídicas que los citados códigos internacionales pueden tener respecto de cada una de estas instituciones y contratos.

  17. Seguridad en el mar, algunas implicaciones de los códigos IGS y PBIB.

    Directory of Open Access Journals (Sweden)

    José Vicente Guzmán Escobar

    2007-01-01

    Full Text Available La expedición del Código Internacional de Gestión de la Seguridad Marítima (Código IGS y del Código de Protección de Buques e Instalaciones Portuarias (Código PBIB, en el contexto del Convenio SOLAS, tiene especiales implicaciones legales en diferentes figuras del derecho marítimo internacional, tales como la limitación de responsabilidad de los armadores de buques por reclamaciones marítimas, la limitación de responsabilidad de los armadores por contaminación por derrames de hidrocarburos, la limitación de responsabilidad del transportador marítimo de mercancías, la obligación del armador y del transportador marítimo en relación con la navegabilidad del buque, los contratos de fletamento y las coberturas de los Clubes de Protección e Indemnización. El presente artículo hace una descripción de las posibles consecuencias jurídicas que los citados códigos internacionales pueden tener respecto de cada una de estas instituciones y contratos.

  18. EL PARADIGMA ENERGÉTICO DE LOS BIOCOMBUSTIBLES Y SUS IMPLICACIONES: PANORAMA MUNDIAL Y EL CASO COLOMBIANO

    Directory of Open Access Journals (Sweden)

    CASTIBLANCO ROZO CARMENZA

    2012-12-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Ante la necesidad mundial por encontrar fuentes de energía alternativas, los biocombustibles se han constituido en la última década como una posible respuesta. Su creciente demanda se ha visto reflejada en la expansión de los cultivos utilizados como materias primas, lo cual tiene implicaciones en varios frentes: los cambios de uso de la tierra, servicios ecosistémicos relacionados con el recurso hídrico, las emisiones de Gases Efecto Invernadero (GEI, la seguridad alimentaria y la propiedad de la tierra. El presente artículo tiene como objetivo presentar un análisis crítico de estos temas a partir de la revisión de la literatura internacional y abordar el caso colombiano, en particular la expansión de los cultivos de palma africana y sus implicaciones sociales. Los principales resultados dejan ver que la expansión de los cultivos se direccionará hacia los países del trópico, lo cual genera  impactos negativos al intensificar la competencia por el agua y la tierra en regiones caracterizadas por su alta vulnerabilidad ecosistémica y social. Adicionalmente se observa la necesidad de mejorar los sistemas de información y de refinar metodologías y modelos que permitan evaluar con mayor precisión los impactos de la producción de biocombustibles sobre el bienestar humano.

  19. Vulnerabilidade das crianças com necessidades especiais de saúde: implicações para a enfermagem Vulnerabilidad de los niños con necesidades especiales de atención médica: implicaciones para enfermería Vulnerability of children with special health care needs: implications for nursing

    Directory of Open Access Journals (Sweden)

    Andressa da Silveira

    2012-12-01

    Full Text Available Trata-se de uma pesquisa qualitativa, descritiva e exploratória que objetivou descrever a vulnerabilidade das crianças com necessidades especiais de saúde para o cuidado e manutenção da vida no cotidiano. Os sujeitos foram 10 familiares/cuidadores de crianças em uma unidade de internação pediátrica de um hospital de ensino. Os dados foram produzidos por meio do método criativo e sensível, mediado pelas dinâmicas de criatividade e sensibilidade e submetidos à análise de discurso francesa. Os resultados apontaram que as crianças possuem vulnerabilidade individual, social e programática traduzidas pela sua fragilidade clínica, a dificuldade de acesso aos serviços de saúde e a inexistência de políticas públicas específicas. Recomenda-se a reestruturação do sistema de referência e contrarreferência, programas e políticas públicas de saúde específicas para essa clientela. Para isso, torna-se relevante que os profissionais de enfermagem proporcionem, aos familiares/cuidadores, momentos de escuta, reconhecendo na subjetividade do cuidado desenvolvido pelo familiar no domicílio.Estudio cualitativo descriptivo y exploratorio que tuvo como objetivo describir la vulnerabilidad de los niños con necesidades especiales de salud para el cuidado cotidiano. Los sujetos fueron 10 familiares/cuidadores de niños en una unidad de pediatría de un hospital universitario. Los datos se produjeron por medio del método creativo y sensible a través de la dinámica de la creatividad y sensibilidad y sometidos al análisis francés del discurso. Los resultados mostraron que los niños tienen vulnerabilidad individual, social, y programática traducidas por su fragilidad clínica, la dificultad de acceso a los servicios de salud y la falta de políticas específicas. Se recomienda la reestructuración del sistema de referencia y contrarreferencia, los programas y las políticas públicas de salud para que estos niños. Es importante que los

  20. Proyecto innovador: “con+ciencia”

    OpenAIRE

    Fernández Arroyo, Jorge; Rodríguez Marín, Fátima; González Medina, Sara

    2013-01-01

    En esta comunicación se expone la experiencia de observación sobre el funcionamiento del proyecto Con+ciencia en los cursos de 5º y 6º de Primaria (tercer ciclo) del centro C.E.I.P. “Príncipe de Asturias” (Sevilla) durante el mes de marzo del 2013. Nuestra participación ha buscado conocer en profundidad este proyecto, observar su aplicación real en el aula y sus implicaciones en el alumnado y el profesorado.

  1. ADENOVIRUS INTERACTION WITH ITS CELLULAR RECEPTOR CAR.

    Energy Technology Data Exchange (ETDEWEB)

    HOWITT,J.; ANDERSON,C.W.; FREIMUTH,P.

    2001-08-01

    The mechanism of adenovirus attachment to the host cell plasma membrane has been revealed in detail by research over the past 10 years. It has long been known that receptor binding activity is associated with the viral fibers, trimeric spike proteins that protrude radially from the vertices of the icosahedral capsid (Philipson et al. 1968). In some adenovirus serotypes, fiber and other virus structural proteins are synthesized in excess and accumulate in the cell nucleus during late stages of infection. Fiber protein can be readily purified from lysates of cells infected with subgroup C viruses, for example Ad2 and Ad5 (Boulanger and Puvion 1973). Addition of purified fiber protein to virus suspensions during adsorption strongly inhibits infection, indicating that fiber and intact virus particles compete for binding sites on host cells (Philipson et al. 1968; Hautala et al. 1998). Cell binding studies using purified radiolabeled fiber demonstrated that fiber binds specifically and with high affinity to the cell plasma membrane, and that cell lines typically used for laboratory propagation of adenovirus have approximately 10{sup 4} high-affinity receptor sites per cell (Persson et al. 1985; Freimuth 1996). Similar numbers of high-affinity binding sites for radiolabeled intact virus particles also were observed (Seth et al. 1994).

  2. Implicaciones de las nanotecnologías en el empleo

    Directory of Open Access Journals (Sweden)

    Noela Invernizzi

    2016-02-01

    Full Text Available Las investigaciones sobre las implicaciones sociales de las nanotecnologías no han abordado el tema del empleo, a pesar de su importancia social. A partir del análisis de las características de los productos de la nanotecnología que están en el mercado, este artículo muestra que las nanotecnologías tendrán un impacto considerable en la distribución del empleo entre los sectores, en la división internacional del trabajo y en las calificaciones para las actividades, causando efectos desestabilizadores en el empleo a nivel nacional y global.

  3. The search for adenovirus 14 in children in Houston, Texas.

    Science.gov (United States)

    Laham, Federico R; Jewell, Alan M; Schoonover, Shauna L; Demmler, Gail J; Piedra, Pedro A

    2008-07-01

    Adenovirus (Ad)14 has recently emerged in the United States causing outbreaks of severe respiratory disease. To determine if Ad14 circulated in Houston, Texas, during the same time as an outbreak in military recruits in nearby San Antonio, 215 pediatric adenovirus isolates were serotyped using microneutralization. None were Ad14; Ad1, Ad2, and Ad3 were the most common identified serotypes.

  4. Migración irregular de haitianos hacia república dominicana y sus implicaciones socio-económicas en el desarrollo de ambas naciones

    OpenAIRE

    Ovalles Mejía, José Manuel; Uribe Mendoza, Cristhian José

    2017-01-01

    84 páginas : gráficos. Esta investigación analiza la migración irregular de haitianos hacia la República Dominicana y sus implicaciones socio-económicas en el desarrollo de ambas naciones durante el período 2010-2015. Este tema reviste gran importancia en el entendido de que los dos países analizados, con condiciones económicas, políticas, sociales y culturales con marcadas diferencias, hoy día comparten y conviven en una misma isla a causa del fenómeno de la migración. Se hace una des...

  5. Alternate adenovirus type-pairs for a possible circumvention of host immune response to recombinant adenovirus vectors.

    Science.gov (United States)

    Nász, I; Adám, E; Lengyel, A

    2001-01-01

    With the help of monoclonal antibodies the existence of at least 18 different earlier not known intertype (IT) specific epitopes were demonstrated in different numbers and combinations on the hexons of different adenovirus serotypes. The IT specific epitopes play an important role in the experimental gene therapy and in the recombinant adenovirus vaccination because of the harmful immune response of the recipient organisms directed against the many different epitopes of the adenovirus vector. For the elimination of harmful effect the authors suggest the use of multiple vectors, each prepared from different adenovirus serotypes showing the loosest antigenic relationship to each other. The vectors would be used sequentially when second or multiple administration is needed. For this purpose the authors determined and described 31 such adenovirus type-pairs, which are probably the best alternates for sequential use in experimental gene therapy.

  6. Prevalencia y caracterización de la vejiga hiperactiva detectada en una población de Madrid con el cuestionario OAB-V3 autoadministrado en atención primaria

    Directory of Open Access Journals (Sweden)

    Javier C. Angulo

    2018-02-01

    Conclusiones: OAB-V3 es un cuestionario simple para el cribado de VH con buena exactitud de predicción en atención primaria y que conlleva implicaciones importantes en la calidad de vida relacionada con la salud.

  7. Los tipos de contratación laboral y sus implicaciones en el contrato psicológico

    Directory of Open Access Journals (Sweden)

    Juan Javier Vesga Rodríguez

    2011-01-01

    Full Text Available En el presente artículo se presentan algunas reflexiones, con bases teóricas y empíricas, acerca de las implicaciones que están teniendo lugar en la conformación de los contratos psicológicos de los trabajadores que se encuentran vinculados mediante empleo y formas contemporáneas de contratación, como el outsourcing y las cooperativas de trabajo asociado. Estudios recientes muestran cómo estas otras formas de contratación afectan aspectos psicosociales de los trabajadores, impactando de manera negativa la gestión de las organizaciones. El artículo finaliza con una serie de interrogantes acerca de la confluencia entre los efectos negativos en el contrato psicológico de las formas de tercerización de la fuerza de trabajo, y tendencias actuales de gestión como los equipos de alto desempeño y la gestión del conocimiento.

  8. Diversidad genética de Plasmodium falciparum y sus implicaciones en la epidemiología de la malaria.

    Directory of Open Access Journals (Sweden)

    Judy Natalia Jiménez

    2005-12-01

    Full Text Available La diversidad genética le confiere a Plasmodium falciparum la capacidad de evadir la respuesta inmune del hospedero y producir variantes resistentes a medicamentos y a vacunas, aspectos que juegan un papel importante en el establecimiento de medidas de control contra la malaria. Diferentes autores han documentado la existencia de diversas cepas o clones de P. falciparum, cuya diversidad genética se ha confirmado a través de distintos ensayos de PCR (reacción en cadena de la polimerasa. Numerosas investigaciones realizadas en poblaciones con diferente grado de transmisión de malaria han mostrado la relación existente entre la estructura de la población de P. falciparum y la epidemiología de la enfermedad. En este artículo se describen las fases del ciclo de vida en las que los eventos de recombinación originan la diversidad genética de P. falciparum, se revisan los estudios realizados sobre este aspecto en regiones con diferentes grados de endemicidad, así como sobre sus implicaciones en la adquisición de inmunidad y en el desarrollo de medidas de control.

  9. Teoría de los marcos relacionales: algunas implicaciones para la psicopatología y la psicoterapia

    Directory of Open Access Journals (Sweden)

    Serafín Gómez-Martín

    2007-01-01

    Full Text Available El objetivo de este estudio teórico es destacar las posibilidades aplicadas de la Teoría de los Marcos Relacionales (RFT en el ámbito clínico. En primer lugar, describiremos de manera muy breve algunos de los principales conceptos técnicos de la teoría (por ejemplo, los conceptos de transformación de funciones y de operante generalizada. Posteriormente, propondremos cuatro causas por las cuales se generan los problemas psicológicos derivadas de una aproximación a la psicopatología humana generada desde la RFT. Dichas causas tienen que ver con los contextos verbales de la literalidad, la formación de categorías y por consiguiente la evaluación y comparación, el contexto de la búsqueda de coherencia y de dar razones y finalmente con el control de los eventos privados. Por último, se expondrán las amplias implicaciones clínicas de los conceptos de contexto relacional y contexto funcional, usando ejemplos clínicos relevantes y clasificando diferentes terapias en función de cual es su objetivo principal: la alteración del contexto funcional o del contexto relacional de redes relacionales.

  10. Development of replication-deficient adenovirus malaria vaccines.

    Science.gov (United States)

    Hollingdale, Michael R; Sedegah, Martha; Limbach, Keith

    2017-03-01

    Malaria remains a major threat to endemic populations and travelers, including military personnel to these areas. A malaria vaccine is feasible, as radiation attenuated sporozoites induce nearly 100% efficacy. Areas covered: This review covers current malaria clinical trials using adenoviruses and pre-clinical research. Heterologous prime-boost regimens, including replication-deficient human adenovirus 5 (HuAd5) carrying malaria antigens, are efficacious. However, efficacy appears to be adversely affected by pre-existing anti-HuAd5 antibodies. Current strategies focus on replacing HuAd5 with rarer human adenoviruses or adenoviruses isolated from non-human primates (NHPs). The chimpanzee adenovirus ChAd63 is undergoing evaluation in clinical trials including infants in malaria-endemic areas. Key antigens have been identified and are being used alone, in combination, or with protein subunit vaccines. Gorilla adenoviruses carrying malaria antigens are also currently being evaluated in preclinical models. These replacement adenovirus vectors will be successfully used to develop vaccines against malaria, as well as other infectious diseases. Expert commentary: Simplified prime-boost single shot regimens, dry-coated live vector vaccines or silicon microneedle arrays could be developed for malaria or other vaccines. Replacement vectors with similar or superior immunogenicity have rapidly advanced, and several are now in extensive Phase 2 and beyond in malaria as well as other diseases, notably Ebola.

  11. Adrenal gland infection by serotype 5 adenovirus requires coagulation factors.

    Directory of Open Access Journals (Sweden)

    Lucile Tran

    Full Text Available Recombinant, replication-deficient serotype 5 adenovirus infects the liver upon in vivo, systemic injection in rodents. This infection requires the binding of factor X to the capsid of this adenovirus. Another organ, the adrenal gland is also infected upon systemic administration of Ad, however, whether this infection is dependent on the cocksackie adenovirus receptor (CAR or depends on the binding of factor X to the viral capsid remained to be determined. In the present work, we have used a pharmacological agent (warfarin as well as recombinant adenoviruses lacking the binding site of Factor X to elucidate this mechanism in mice. We demonstrate that, as observed in the liver, adenovirus infection of the adrenal glands in vivo requires Factor X. Considering that the level of transduction of the adrenal glands is well-below that of the liver and that capsid-modified adenoviruses are unlikely to selectively infect the adrenal glands, we have used single-photon emission computed tomography (SPECT imaging of gene expression to determine whether local virus administration (direct injection in the kidney could increase gene transfer to the adrenal glands. We demonstrate that direct injection of the virus in the kidney increases gene transfer in the adrenal gland but liver transduction remains important. These observations strongly suggest that serotype 5 adenovirus uses a similar mechanism to infect liver and adrenal gland and that selective transgene expression in the latter is more likely to be achieved through transcriptional targeting.

  12. Desarrollo multinivel: Implicaciones macro-regional, local y micro- regional

    Directory of Open Access Journals (Sweden)

    José Guadalupe Vargas Hernández

    2006-11-01

    Full Text Available Este trabajo se propone analizar las implicaciones del desarrollo en los niveles macro-regional, local y micro- regional, a partir de la hipótesis central de la teoría del desarrollo que plantea que el desarrollo económico traerá consigo el desarrollo político y social. Después de hacer de un acercamiento conceptual al desarrollo, se repasan brevemente las teorías del desarrollo existentes como herramientas de análisis de la realidad. En la discusión se concluye que los procesos de desarrollo locales y regionales requieren de una transformación sustancial de las relaciones negociadas entre los agentes económicos y los actores políticos. Ante el paulatino retroceso que en las sociedades contemporáneas está teniendo el Estado de bienestar, uno de los principales retos es el empoderamiento de las organizaciones sociales y comunitarias para que desempeñen activamente su rol en los procesos de desarrollo local y regional.

  13. Adenovirus Infection in Children with Diarrhea Disease in ...

    African Journals Online (AJOL)

    Ad40) and type 41(Ad41), can cause acute and severe diarrhea in young children worldwide. This study was conducted to delineate the epidemiological features of adenoviruses identified in children with gastroenteritis in Northwestern Nigeria.

  14. Human adenovirus-36 and childhood obesity.

    Science.gov (United States)

    Atkinson, Richard L

    2011-09-01

    There is increasing evidence that obesity in humans is associated with infection with human adenovirus-36 (Adv36). Infection of experimental animals with Adv36 demonstrates that this virus causes obesity. Human studies have shown a prevalence of Adv36 infection of 30% or greater in obese adult humans, but a correlation with obesity has not always been demonstrated. In contrast, three published studies and one presented study with a total of 559 children all show that there is an increase in prevalence of Adv36 infection in obese children (28%) compared to non-obese children (10%). The explanation for the apparently more robust correlation of Adv36 infection with obesity in children vs. adults is not clear. The data in animals and people suggests that Adv36 has contributed to the worldwide increase in childhood obesity. More research is needed to identify prevalences and consequences of Adv36 infection in people of all age groups and geographic locations.

  15. Adenovirus 36 DNA in human adipose tissue.

    Science.gov (United States)

    Ponterio, E; Cangemi, R; Mariani, S; Casella, G; De Cesare, A; Trovato, F M; Garozzo, A; Gnessi, L

    2015-12-01

    Recent studies have suggested a possible correlation between obesity and adenovirus 36 (Adv36) infection in humans. As information on adenoviral DNA presence in human adipose tissue are limited, we evaluated the presence of Adv36 DNA in adipose tissue of 21 adult overweight or obese patients. Total DNA was extracted from adipose tissue biopsies. Virus detection was performed using PCR protocols with primers against specific Adv36 fiber protein and the viral oncogenic E4orf1 protein nucleotide sequences. Sequences were aligned with the NCBI database and phylogenetic analyses were carried out with MEGA6 software. Adv36 DNA was found in four samples (19%). This study indicates that some individuals carry Adv36 in the visceral adipose tissue. Further studies are needed to determine the specific effect of Adv36 infection on adipocytes, the prevalence of Adv36 infection and its relationship with obesity in the perspective of developing a vaccine that could potentially prevent or mitigate infection.

  16. Las implicaciones del Brexit en los derechos de libre circulación de personas, laborales y de residencia de los ciudadanos de la Unión Europea: el Modelo de Suiza Modificado como la opción más viable para el Reino Unido en la negociación de un nuevo acuerdo con la Unión Europea.

    OpenAIRE

    Lozano Martínez, Bárbara

    2017-01-01

    Un análisis profundo de los derechos de libre circulación de personas, laborales y de residencia de los ciudadanos de la Unión Europea, para poosteriormente analizarlos en distintos escenarios que el Reino Unido podría tomar como base para un nuevo acuerdo con la Unión Europea al formalizarse el Brexit.

  17. Acute Hepatitis and Pancytopenia in Healthy Infant with Adenovirus

    Directory of Open Access Journals (Sweden)

    Amr Matoq

    2016-01-01

    Full Text Available Adenoviruses are a common cause of respiratory infection, pharyngitis, and conjunctivitis in infants and young children. They are known to cause hepatitis and liver failure in immunocompromised patients; they are a rare cause of hepatitis in immunocompetent patients and have been known to cause fulminant hepatic failure. We present a 23-month-old immunocompetent infant who presented with acute noncholestatic hepatitis, hypoalbuminemia, generalized anasarca, and pancytopenia secondary to adenovirus infection.

  18. Production of Recombinant Adenovirus Containing Human Interlukin-4 Gene

    OpenAIRE

    Mojarrad, Majid; Abdolazimi, Yassan; Hajati, Jamshid; Modarressi, Mohammad Hossein

    2011-01-01

    Objective(s) Recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. Ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. In the present experiment, a recombinant adenovirus containing human IL-4 coding sequence was made. IL-4 has several characteristics ...

  19. [Construction and expression of a recombinant adenovirus with LZP3].

    Science.gov (United States)

    Chen, Bang-dang; Zhang, Fu-chun; Sun, Mei-yu; Li, Yi-jie; Ma, Zheng-hai

    2007-08-01

    To explore a new immunocontraceptive vaccine and construct an attenuated recombinant adenoviral vaccine against Lagurus lagurus zona pellucida 3(LZP3). LZP3 gene was subcloned into the shuttle vector pShuttle-CMV, and then a two-step transformation procedure was employed to construct a recombinant adenoviral plasmid with LZP3, which was digested with Pac I and transfected into HEK293 cells to package recombinant adenovirus particles. Finally, HeLa cells were infected by the recombinant adenovirus. LZP3 gene was detected from the recombinant virus by PCR, and its transcription and expression were analyzed by RT-PCR and Western blot. Recombinant adenovirus vector pAd-LZP3 with LZP3 gene was constructed by homologous recombination in E.coli, and a recombinant adenovirus was obtained by transfecting HEK293 cells with pAd-LZP3. PCR test indicated that LZP3 gene was successfully integrated into the adenoviral genome, and the titer of the recombinant adenovirus reached 1.2x10(10) pfu/L. The transcription and expression of LZP3 gene in the infected HeLa cells were confirmed by RT-PCR and Western blot. The recombinant adenovirus RAd-LZP3 can be successfully expressed in the infected HeLa cells, which lays the foundation for further researches into immunizing animals with RAd-LZP3.

  20. Trabajo creativo y reestructuración productiva en la industria cultural de la publicidad farmacéutica online en el marco del capitalismo contemporáneo : implicaciones socioeconómicas en Argentina, 2008 - 2012

    OpenAIRE

    Ruiz, Silvina

    2013-01-01

    Esta Tesis se propone contribuir al conocimiento sobre la organización productiva y del trabajo que denominamos “Creativo / Original” vs. "Creativo / Comercial”, en la producción de contenidos informacionales en la Industria Cultural de la Publicidad Farmacéutica online en la Argentina, y sus implicaciones para el desarrollo de los/as trabajadores/as y del país. Con ese objetivo nuestra investigación -si bien se concentra en el nivel micro social en base a la experiencia de una empresa trasna...

  1. La ética y la técnica contemporánea: Implicaciones en el Área de la Salud

    Directory of Open Access Journals (Sweden)

    Gladys Santos-Falcón

    2005-03-01

    Full Text Available Este artículo tiene el objetivo de propiciar, a partir de las ideas de algunos autores, una reflexión y discusión en los profesionales de la salud sobre las implicaciones en la práctica cotidiana de los avances de la técnica, así como sobre el papel de la ética como ente regulador de estos avances. También se propone analizar los cambios que se han generado en las relaciones entre los profesionales y las personas/clientes de los servicios de salud, a partir de la biotecnología. Se propone este análisis con la finalidad de incorporar en nuestra práctica cotidiana la ética por la vida, por la libertad de vivir mejor, respetando la naturaleza y la dignidad humana.

  2. Implicaciones de los conceptos actuales neuropsicológicos de la memoria en el aprendizaje y en la enseñanza

    Directory of Open Access Journals (Sweden)

    Javier de la Fuente Rocha

    2015-01-01

    Full Text Available Se hace una revisión de los conocimientos neuropsicológicos de procesos involucrados en el aprendizaje y, a partir de ellos, se aplica el método analítico-sintético para comentar algunas de sus implicaciones en la metodología pedagógica. Esta revisión presenta conceptos actuales que relacionan los centros nerviosos participantes, las vías que los conectan y su integración con otras funciones cerebrales importantes en el proceso de aprendizaje. Estos conocimientos tienen importancia en el área educativa debido a su potencial para dar sustento a diversas estrategias útiles en el proceso de enseñanza-aprendizaje.

  3. LA HIPÓTESIS DEL TRAUMA EN EL ABUSO SEXUAL: REVISIÓN CRÍTICA E IMPLICACIONES

    Directory of Open Access Journals (Sweden)

    Agustín Malón Marco

    2008-07-01

    Full Text Available Se lleva a cabo una revisión crítica de la hipótesis traumática en el estudio de las experiencias eróticas tempranas con personas más mayores. Esta hipótesis, la más habitual en la literatura científica, consiste en dar por cierto que son en su mayoría experiencias graves y de serias consecuencias negativas a corto y/o largo plazo. Cuestionaremos su fundamentación empírica, para después pasar a las implicaciones científicas y profesionales surgidas de otra lectura de los datos disponibles. Para ello se analizan críticamente nueve revisiones previas sobre la cuestión, publicadas entre 1981 y 1998. Esta revisión de revisiones sugiere que estaría perfectamente sustentada la hipótesis de que en la gran mayoría de los casos no suelen producirse daños serios ni mucho menos permanentes en los implicados. El análisis invita a un replanteamiento teórico, epistemológico y tecnológico de la investigación y la intervención social.

  4. Violencia de Pareja en Mujeres Hispanas: Implicaciones para la Investigación y la Práctica

    Science.gov (United States)

    Gonzalez-Guarda, Rosa Maria; Becerra, Maria Mercedes

    2012-01-01

    Las investigaciones sobre la violencia entre parejas sugieren que las mujeres hispanas están siendo afectadas desproporcionadamente por la ocurrencia y consecuencias de este problema de salud pública. El objetivo del presente artículo es dar a conocer el estado del arte en relación a la epidemiologia, consecuencias y factores de riesgo para VP entre mujeres Hispanas, discutiendo las implicaciones para la investigación y la práctica. Investigaciones han demostrado una fuerte asociación del status socioeconómico, abuso de droga y el alcohol, la salud mental, aculturación, inmigración, comportamientos sexuales riesgosos e historia de abuso con la violencia entre parejas. Sin embargo, más estudios se deben llevar a cabo para identificar otros factores de riesgos y de protección a poblaciones hispanas no clínicas. Mientras que el conocimiento sobre la etiología de la VP entre mujeres Hispanas se expanda, enfermeras y otros profesionales de la salud deben desarrollar, implementar y evaluar estrategias culturalmente adecuadas para la prevención primaria y secundaria de la violencia entre pareja. PMID:26166938

  5. Perspectiva no androcéntrica en los estudios sobre familias monoparentales. Reflexiones e implicaciones metodológicas

    Directory of Open Access Journals (Sweden)

    Dino Di Nella

    2014-12-01

    Full Text Available En este trabajo realizamos una reflexión sobre el proceso que nos llevó a la progresiva configuración de un modelo de abordaje metodológico específico para la investigación sobre las familias monoparentales. Un proceso construido y sistematizado a partir de los aportes de las epistemologías feministas al diseño metodológico y a las formas de trabajo participativas. De ello derivo una contribución científico-técnica relevante e inédita hasta la actualidad a nivel internacional: la Encuesta sobre Monoparentalidad y diversidad familiar (EMODIF, la cual proponemos como un instrumento de medición no androcéntrico de las monoparentalidades, sus perfiles, experiencias, expectativas y realidades. Con este artículo queremos ofrecer una sistematización de las implicaciones que ha tenido nuestra aplicación y materialización de la perspectiva feminista en los estudios de las familias monoparentales.

  6. Cabeza hacia adelante: una mirada desde la biomecánica y sus implicaciones sobre el movimiento corporal humano

    Directory of Open Access Journals (Sweden)

    Iván Darío Pinzón Ríos

    2015-04-01

    Full Text Available Las condiciones ergonómicas de mobiliario y de equipos, como también la postura incorrecta al estar en posición sedente, de pie o cargando algún peso pueden generar dolores o complicaciones osteomusculares. Dentro de estas, la cabeza hacia adelante es una alteración común detectada en la evaluación postural realizada por los fisioterapeutas. Esta suele asociarse a cambios mecánicos del cuello y columna vertebral, los cuales alteran la funcionalidad de los individuos, causándole discapacidad pues compromete el movimiento de hombros, la movilidad de la articulación temporomandibular, la mecánica respiratoria, desarrollando imbalances musculares, mareos, disfunción vestibular, aumenta el riesgo de fracturas y se asocia con procesos dolorosos como la cervicalgia, dorsalgia y lumbalgia. Por tanto el siguiente artículo tiene por objetivo revisar la fisiopatogénia de la cabeza hacia adelante, desde una perspectiva biomecánica, analizando las implicaciones de esta alteración postural sobre el movimiento corporal humano, proporcionando así herramientas de análisis para el abordaje fisioterapéutico.

  7. Functional Interaction of the Adenovirus IVa2 Protein with Adenovirus Type 5 Packaging Sequences

    OpenAIRE

    Ostapchuk, Philomena; Yang, Jihong; Auffarth, Ece; Hearing, Patrick

    2005-01-01

    Adenovirus type 5 (Ad5) DNA packaging is initiated in a polar fashion from the left end of the genome. The packaging process is dependent on the cis-acting packaging domain located between nucleotides 230 and 380. Seven AT-rich repeats that direct packaging have been identified within this domain. A1, A2, A5, and A6 are the most important repeats functionally and share a bipartite sequence motif. Several lines of evidence suggest that there is a limiting trans-acting factor(s) that plays a ro...

  8. La problematica de la demarcacion entre ciencia y pseudociencia y sus implicaciones en la educacion cientifica

    Science.gov (United States)

    Jimenez Tolentino, Dinorah

    2011-12-01

    En la sociedad prevalece una tendencia generalizada hacia la inclusion de creencias y practicas pseudocientificas. Esta investigacion responde a la necesidad de analizar como la proliferacion de las pseudociencias afecta la vision que tienen los estudiantes universitarios sobre las ciencias naturales. A tales efectos, la investigadora describe las concepciones epistemologicas que tienen los estudiantes sobre las ciencias y las pseudociencias e identifica los criterios de demarcacion, entre un area y otra, que se derivan de estas concepciones. De igual modo, esta identifica las creencias y practicas pseudocientificas de mayor arraigo entre los estudiantes, destacando, a su vez, la razon de ser de las mismas. Por ultimo, la investigadora analiza las implicaciones educativas de la problematica de la demarcacion entre ciencia y pseudociencia. La investigacion es de naturaleza mixta, enmarcada en los paradigmas empirico- analitico y cualitativo. El proceso investigativo se llevo a cabo mediante la administracion del cuestionario Criterios para la demarcacion entre ciencia y pseudociencia. La parte cualitativa estuvo enmarcada en el diseno de estudio de caso, recopilando informacion mediante entrevistas semiestructuradas en dos grupos focales. La poblacion de estudio estuvo constituida por estudiantes universitarios del nivel subgraduado de la Universidad Central de Bayamon. Los resultados del estudio reflejaron las concepciones erroneas de los estudiantes sobre la naturaleza de las ciencias y las pseudociencias. Con respecto a la demarcacion entre ciencia y pseudociencia, el criterio imperante entre los universitarios es el de la verificabilidad, considerando la aplicacion del metodo cientifico como el metodo para demostrar la veracidad de las teorias cientificas. Las creencias y practicas pseudocientificas no son muy frecuentes entre los universitarios. Estos atribuyen las mismas a la prevalencia de elementos supersticiosos y al engano a que es sometida la poblacion

  9. Sylos Labini sobre Marx: implicaciones para la política económica

    Directory of Open Access Journals (Sweden)

    Massimo Cingolani

    2016-09-01

    Full Text Available La presente colaboración aborda algunos aspectos postulados por Sylos Labini en su interpretación de la economía de Carlos Marx, sin duda polémica pero necesaria. Para ello se retoma la amplia discusión teórica realizada en torno a esos temas por varios académicos, que de una manera u otra, han participado e influido en dicho debate. Esta reflexión, importante en sí misma, se vuelve imprescindible en referencia a determinadas implicaciones de gran envergadura sobre la aplicación de la política económica impuesta en los últimos más de 25 años en la Unión Europea, bajo los estrechos criterios de la política fiscal y monetaria impuesta. El autor expresa su opinión a título personal. Este ensayo es una traducción realizada por Glenda Lao Largaespada y Michele Cingolani y revisada por el autor de un artículo publicado con el mismo título en el vol. 68, n. 269 (2015, pp. 81- 147 de Moneta e Credito. El trabajo desarrolla un informe presentado el 25 de Octubre de 2013 en Bolonia en la 54a Reunión Científica Anual de la Sociedad Italiana de los Economistas, que se desarrolló en una lección en la Universidad de Bérgamo el 9 de Mayo de 2014. Sin implicar su adhesión a las tesis presentadas, el autor desea agradecer a Jean-Luc Bailly, Riccardo Bellofiore, Claude Berthomieu, Luca Cefisi, Nadia Garbellini, Lorenzo Esposito, Joseph Halevi, Eckhard Hein, Stefano Lucarelli, Fabio Petri, Antonella Rancan, Roberto Romano, Alessandro Roncaglia, Andrea Salanti, Claudio Sardoni, Andrew Trigg, Jan Toporowski, Ariel Wirkierman, los referees anónimos y los traductores por los comentarios, críticas y apoyo recibidos. El autor asume plena responsabilidad por los errores que persistan.

  10. Multicultura e interculturalidad: implicaciones de una ausencia en la educación

    Directory of Open Access Journals (Sweden)

    Fernando Carlos Aguero-Contreras

    2016-06-01

    Full Text Available Resumen Reflexión teórica desde las perspectivas sociológica – antropológica de la educación, sustentada en los enfoques de la multicultura e interculturalidad. Se parte de varios estudios realizados en la residencia estudiantil de la Universidad de Cienfuegos, al centro - sur de Cuba. Estos han combinados análisis cuantitativos y cualitativos, considerando variables e indicadores socio demográficos, relacionados con la satisfacción en el acceso a la cultura, la salud, las relaciones y dinámicas sociales, mediadas por una estructura social clasista y sociocultural que distingue especificidades en la biografía cultural de individuos y grupos. Se concedió importancia a la significación dada a la residencia como escenario relevante de la vida educacional, las percepciones de las relaciones múltiples y diversas entre los grupos que tienen presencia en ella, así como los niveles de diálogos en los ámbitos académicos, científicos y sociales. Se analizaron varios documentos, y se realizaron observaciones, entrevistas estructuradas y en profundidad. Desde este contexto se muestran las implicaciones del déficit teórico en la gestión formativa en la residencia universitaria, limitando el alcance del proyecto educativo como concreción curricular, para el desarrollo de procesos socializadores más coherentes. Se concluye que la ausencia de estas perspectivas teóricas en la educación superior posibilitaron que la visión política ocupara la primera interpretación del proceso, expresada en la acción solidaria y aun cuando se muestran resultados concretos de importancia en cuanto al número de alumnos graduados, el proceso formativo fue limitado en el diálogo profundo, la socialización cultural para la lograr la unidad en la diversidad.

  11. Implicaciones metabólicas del consumo exesivo de fructosa

    OpenAIRE

    Esquivel-Solís, Viviana; Gómez-Salas, Georgina

    2007-01-01

    En los últimos años se ha dado un auge importante en la producción de alimentos endulzados con edulcorantes distintos a la sacarosa o azúcar de mesa, que por aportar menos calorías, son utilizados ya sea para perder peso o para mantener un peso saludable, o bien, para ofrecer opciones alimenticias a individuos que padecen de diabetes o intolerancia a la glucosa. Uno de los edulcorantes de mayor utilización es el jarabe de maíz alto en fructosa, el cual, en comparación con la sacarosa, tiene u...

  12. Obesidad y Mercado de Trabajo: Factores Determinantes e Implicaciones

    OpenAIRE

    Benítez Llamazares, Nuria

    2016-01-01

    El sobrepeso y la obesidad se definen de acuerdo con la Organización Mundial de la Salud (OMS) como “una acumulación anormal o excesiva de grasa que puede ser perjudicial para la salud”. Ambos conceptos, sobrepeso y obesidad, son considerados factores determinantes de riesgo en la salud de la población y en las últimas décadas hemos visto cómo los porcentajes de población con sobrepeso y obesidad se han incrementado de forma alarmante, especialmente en los países desarrollados....

  13. ORIENTACIÓN DE META DE LOS ENTRENADORES Y METODOLOGÍA DE ENTRENAMIENTO: IMPLICACIONES MOTIVACIONALES

    Directory of Open Access Journals (Sweden)

    J.F. Guzmán

    2010-09-01

    Full Text Available

     

    RESUMEN

    En el presente estudio administramos a 34 entrenadores de balonmano un cuestionario de medida de los criterios de éxito deportivo (POSQ y otro de la importancia otorgada a diferentes variables de la metodología de entrenamiento con el fin de analizar el papel predictor de la orientación de meta de los entrenadores sobre la valoración de aspectos determinantes del entrenamiento. Los resultados de los Análisis de Regresión indicaron que la orientación a la tarea se correspondía con la concesión de importancia a: La especificidad del entrenamiento; la agrupación de los jugadores según su nivel de destreza; el control y anotación de los progresos de los jugadores; la libertad del jugador para ejecutar y decidir; y la consideración de la competición como un medio y no como un fin. La orientación al ego predijo positivamente la concesión de importancia a la variedad de ejercicios y la comunicación con los padres para resolver problemas y negativamente la concesión de importancia a la libertad del jugador para ejecutar y decidir. Las implicaciones motivacionales de estos resultados se analizan a la luz de la teoría de la motivación de logro (Nicholls, 1984a,b y la teoría de la autodeterminación (Deci y Ryan, 1985, 1991.
    PALABRAS CLAVE: Motivación de logro, autodeterminación, metodología de entrenamiento.

    ABSTRACT

    In this study 34 handball coaches were administered a questionnaire to measure their criteria of sport success (POSQ and another to measure the importance each placed on different variables of training methodology. The purpose was to analyse the predictor role of coaches' motivational orientation on given importance to determinants of training. Regression analysis results showed

  14. Default assembly of early adenovirus chromatin

    International Nuclear Information System (INIS)

    Spector, David J.

    2007-01-01

    In adenovirus particles, the viral nucleoprotein is organized into a highly compacted core structure. Upon delivery to the nucleus, the viral nucleoprotein is very likely to be remodeled to a form accessible to the transcription and replication machinery. Viral protein VII binds to intra-nuclear viral DNA, as do at least two cellular proteins, SET/TAF-Iβ and pp32, components of a chromatin assembly complex that is implicated in template remodeling. We showed previously that viral DNA-protein complexes released from infecting particles were sensitive to shearing after cross-linking with formaldehyde, presumably after transport of the genome into the nucleus. We report here the application of equilibrium-density gradient centrifugation to the analysis of the fate of these complexes. Most of the incoming protein VII was recovered in a form that was not cross-linked to viral DNA. This release of protein VII, as well as the binding of SET/TAF-Iβ and cellular transcription factors to the viral chromatin, did not require de novo viral gene expression. The distinct density profiles of viral DNA complexes containing protein VII, compared to those containing SET/TAF-Iβ or transcription factors, were consistent with the notion that the assembly of early viral chromatin requires both the association of SET/TAF-1β and the release of protein VII

  15. Latest Insights on Adenovirus Structure and Assembly

    Directory of Open Access Journals (Sweden)

    Carmen San Martín

    2012-05-01

    Full Text Available Adenovirus (AdV capsid organization is considerably complex, not only because of its large size (~950 Å and triangulation number (pseudo T = 25, but also because it contains four types of minor proteins in specialized locations modulating the quasi-equivalent icosahedral interactions. Up until 2009, only its major components (hexon, penton, and fiber had separately been described in atomic detail. Their relationships within the virion, and the location of minor coat proteins, were inferred from combining the known crystal structures with increasingly more detailed cryo-electron microscopy (cryoEM maps. There was no structural information on assembly intermediates. Later on that year, two reports described the structural differences between the mature and immature adenoviral particle, starting to shed light on the different stages of viral assembly, and giving further insights into the roles of core and minor coat proteins during morphogenesis [1,2]. Finally, in 2010, two papers describing the atomic resolution structure of the complete virion appeared [3,4]. These reports represent a veritable tour de force for two structural biology techniques: X-ray crystallography and cryoEM, as this is the largest macromolecular complex solved at high resolution by either of them. In particular, the cryoEM analysis provided an unprecedented clear picture of the complex protein networks shaping the icosahedral shell. Here I review these latest developments in the field of AdV structural studies.

  16. Adenovirus 36 and Obesity: An Overview.

    Science.gov (United States)

    Ponterio, Eleonora; Gnessi, Lucio

    2015-07-08

    There is an epidemic of obesity starting about 1980 in both developed and undeveloped countries definitely associated with multiple etiologies. About 670 million people worldwide are obese. The incidence of obesity has increased in all age groups, including children. Obesity causes numerous diseases and the interaction between genetic, metabolic, social, cultural and environmental factors are possible cofactors for the development of obesity. Evidence emerging over the last 20 years supports the hypothesis that viral infections may be associated with obesity in animals and humans. The most widely studied infectious agent possibly linked to obesity is adenovirus 36 (Adv36). Adv36 causes obesity in animals. In humans, Adv36 associates with obesity both in adults and children and the prevalence of Adv36 increases in relation to the body mass index. In vivo and in vitro studies have shown that the viral E4orf1 protein (early region 4 open reading frame 1, Adv) mediates the Adv36 effect including its adipogenic potential. The Adv36 infection should therefore be considered as a possible risk factor for obesity and could be a potential new therapeutic target in addition to an original way to understand the worldwide rise of the epidemic of obesity. Here, the data indicating a possible link between viral infection and obesity with a particular emphasis to the Adv36 will be reviewed.

  17. Adenovirus 36 and Obesity: An Overview

    Directory of Open Access Journals (Sweden)

    Eleonora Ponterio

    2015-07-01

    Full Text Available There is an epidemic of obesity starting about 1980 in both developed and undeveloped countries definitely associated with multiple etiologies. About 670 million people worldwide are obese. The incidence of obesity has increased in all age groups, including children. Obesity causes numerous diseases and the interaction between genetic, metabolic, social, cultural and environmental factors are possible cofactors for the development of obesity. Evidence emerging over the last 20 years supports the hypothesis that viral infections may be associated with obesity in animals and humans. The most widely studied infectious agent possibly linked to obesity is adenovirus 36 (Adv36. Adv36 causes obesity in animals. In humans, Adv36 associates with obesity both in adults and children and the prevalence of Adv36 increases in relation to the body mass index. In vivo and in vitro studies have shown that the viral E4orf1 protein (early region 4 open reading frame 1, Adv mediates the Adv36 effect including its adipogenic potential. The Adv36 infection should therefore be considered as a possible risk factor for obesity and could be a potential new therapeutic target in addition to an original way to understand the worldwide rise of the epidemic of obesity. Here, the data indicating a possible link between viral infection and obesity with a particular emphasis to the Adv36 will be reviewed.

  18. Adenovirus respiratory tract infections in Peru.

    Directory of Open Access Journals (Sweden)

    Julia S Ampuero

    Full Text Available BACKGROUND: Currently, there is a paucity of data regarding human adenovirus (HAdv circulation in Andean regions of South America. To address this shortcoming, we report the clinical, phylogenetic, and epidemiologic characteristics of HAdv respiratory tract infection from a large sentinel surveillance study conducted among adults and children in Peru. METHODS/PRINCIPAL FINDINGS: Oropharyngeal swabs were collected from participants visiting any of 38 participating health centers, and viral pathogens were identified by immunofluorescence assay in cell culture. In addition, molecular characterization was performed on 226 randomly selected HAdv samples. Between 2000 and 2010, a total of 26,375 participants with influenza-like illness (ILI or severe acute respiratory infection (SARI were enrolled in the study. HAdv infection was identified in 2.5% of cases and represented 6.2% of all viral pathogens. Co-infection with a heterologous virus was found in 15.5% of HAdv cases. HAdv infection was largely confined to children under the age of 15, representing 88.6% of HAdv cases identified. No clinical characteristics were found to significantly distinguish HAdv infection from other respiratory viruses. Geographically, HAdv infections were more common in sites from the arid coastal regions than in the jungle or highland regions. Co-circulation of subgroups B and C was observed each year between 2006 and 2010, but no clear seasonal patterns of transmission were detected. CONCLUSIONS/SIGNIFICANCE: HAdv accounted for a significant fraction of those presenting with ILI and SARI in Peru and tended to affect the younger population disproportionately. Longitudinal studies will help better characterize the clinical course of patients with HAdv in Peru, as well as determine the role of co-infections in the evolution of illness.

  19. Adenovirus respiratory tract infections in Peru.

    Science.gov (United States)

    Ampuero, Julia S; Ocaña, Víctor; Gómez, Jorge; Gamero, María E; Garcia, Josefina; Halsey, Eric S; Laguna-Torres, V Alberto

    2012-01-01

    Currently, there is a paucity of data regarding human adenovirus (HAdv) circulation in Andean regions of South America. To address this shortcoming, we report the clinical, phylogenetic, and epidemiologic characteristics of HAdv respiratory tract infection from a large sentinel surveillance study conducted among adults and children in Peru. Oropharyngeal swabs were collected from participants visiting any of 38 participating health centers, and viral pathogens were identified by immunofluorescence assay in cell culture. In addition, molecular characterization was performed on 226 randomly selected HAdv samples. Between 2000 and 2010, a total of 26,375 participants with influenza-like illness (ILI) or severe acute respiratory infection (SARI) were enrolled in the study. HAdv infection was identified in 2.5% of cases and represented 6.2% of all viral pathogens. Co-infection with a heterologous virus was found in 15.5% of HAdv cases. HAdv infection was largely confined to children under the age of 15, representing 88.6% of HAdv cases identified. No clinical characteristics were found to significantly distinguish HAdv infection from other respiratory viruses. Geographically, HAdv infections were more common in sites from the arid coastal regions than in the jungle or highland regions. Co-circulation of subgroups B and C was observed each year between 2006 and 2010, but no clear seasonal patterns of transmission were detected. HAdv accounted for a significant fraction of those presenting with ILI and SARI in Peru and tended to affect the younger population disproportionately. Longitudinal studies will help better characterize the clinical course of patients with HAdv in Peru, as well as determine the role of co-infections in the evolution of illness.

  20. Adenovirus Respiratory Tract Infections in Peru

    Science.gov (United States)

    Ampuero, Julia S.; Ocaña, Víctor; Gómez, Jorge; Gamero, María E.; Garcia, Josefina; Halsey, Eric S.; Laguna-Torres, V. Alberto

    2012-01-01

    Background Currently, there is a paucity of data regarding human adenovirus (HAdv) circulation in Andean regions of South America. To address this shortcoming, we report the clinical, phylogenetic, and epidemiologic characteristics of HAdv respiratory tract infection from a large sentinel surveillance study conducted among adults and children in Peru. Methods/Principal Findings Oropharyngeal swabs were collected from participants visiting any of 38 participating health centers, and viral pathogens were identified by immunofluorescence assay in cell culture. In addition, molecular characterization was performed on 226 randomly selected HAdv samples. Between 2000 and 2010, a total of 26,375 participants with influenza-like illness (ILI) or severe acute respiratory infection (SARI) were enrolled in the study. HAdv infection was identified in 2.5% of cases and represented 6.2% of all viral pathogens. Co-infection with a heterologous virus was found in 15.5% of HAdv cases. HAdv infection was largely confined to children under the age of 15, representing 88.6% of HAdv cases identified. No clinical characteristics were found to significantly distinguish HAdv infection from other respiratory viruses. Geographically, HAdv infections were more common in sites from the arid coastal regions than in the jungle or highland regions. Co-circulation of subgroups B and C was observed each year between 2006 and 2010, but no clear seasonal patterns of transmission were detected. Conclusions/Significance HAdv accounted for a significant fraction of those presenting with ILI and SARI in Peru and tended to affect the younger population disproportionately. Longitudinal studies will help better characterize the clinical course of patients with HAdv in Peru, as well as determine the role of co-infections in the evolution of illness. PMID:23056519

  1. Adenovirus dodecahedron, as a drug delivery vector.

    Directory of Open Access Journals (Sweden)

    Monika Zochowska

    Full Text Available BACKGROUND: Bleomycin (BLM is an anticancer antibiotic used in many cancer regimens. Its utility is limited by systemic toxicity and dose-dependent pneumonitis able to progress to lung fibrosis. The latter can affect up to nearly 50% of the total patient population, out of which 3% will die. We propose to improve BLM delivery by tethering it to an efficient delivery vector. Adenovirus (Ad dodecahedron base (DB is a particulate vector composed of 12 copies of a pentameric viral protein responsible for virus penetration. The vector efficiently penetrates the plasma membrane, is liberated in the cytoplasm and has a propensity to concentrate around the nucleus; up to 300000 particles can be observed in one cell in vitro. PRINCIPAL FINDINGS: Dodecahedron (Dd structure is preserved at up to about 50 degrees C at pH 7-8 and during dialysis, freezing and drying in the speed-vac in the presence of 150 mM ammonium sulfate, as well as during lyophilization in the presence of cryoprotectants. The vector is also stable in human serum for 2 h at 37 degrees C. We prepared a Dd-BLM conjugate which upon penetration induced death of transformed cells. Similarly to free bleomycin, Dd-BLM caused dsDNA breaks. Significantly, effective cytotoxic concentration of BLM delivered with Dd was 100 times lower than that of free bleomycin. CONCLUSIONS/SIGNIFICANCE: Stability studies show that Dds can be conveniently stored and transported, and can potentially be used for therapeutic purposes under various climates. Successful BLM delivery by Ad Dds demonstrates that the use of virus like particle (VLP results in significantly improved drug bioavailability. These experiments open new vistas for delivery of non-permeant labile drugs.

  2. Genomic stability of adipogenic human adenovirus 36.

    Science.gov (United States)

    Nam, J-H; Na, H-N; Atkinson, R L; Dhurandhar, N V

    2014-02-01

    Human adenovirus Ad36 increases adiposity in several animal models, including rodents and non-human primates. Importantly, Ad36 is associated with human obesity, which has prompted research to understand its epidemiology and to develop a vaccine to prevent a subgroup of obesity. For this purpose, understanding the genomic stability of Ad36 in vivo and in vitro infections is critical. Here, we examined whether in vitro cell passaging over a 14-year period introduced any genetic variation in Ad36. We sequenced the whole genome of Ad36-which was plaque purified in 1998 from the original strain obtained from American Type Culture Collection, and passaged approximately 12 times over the past 14 years (Ad36-2012). This DNA sequence was compared with a previously published sequence of Ad36 likely obtained from the same source (Ad36-1988). Compared with Ad36-1988, only two nucleotides were altered in Ad36-2012: a T insertion at nucleotide 1862, which may induce early termination of the E1B viral protein, and a T➝C transition at nucleotide 26 136. Virus with the T insertion (designated Ad36-2012-T6) was mixed with wild-type virus lacking the T insertion (designated Ad36-2012-T5) in the viral stock. The transition at nucleotide 26 136 does not change the encoded amino acid (aspartic acid) in the pVIII viral protein. The rate of genetic variation in Ad36 is ∼2.37 × 10(-6) mutations/nucleotide/passage. Of particular importance, there were no mutations in the E4orf1 gene, the critical gene for producing obesity. This very-low-variation rate should reduce concerns about genetic variability when developing Ad36 vaccines or developing assays for detecting Ad36 infection in populations.

  3. e implicaciones para el bienestar de los humanos

    Directory of Open Access Journals (Sweden)

    Germán Gutiérrez

    2007-01-01

    Full Text Available En este artículo se revisa la literatura reciente sobre interacciones humano-animal, su origen, sus características y cómo dichas interacciones afectan el bienestar físico, psicológico y social. En la primera parte se presentan los orígenes y evolución histórica de las relaciones humano - animal, empezando por los procesos de domesticación y se aborda la naturaleza de las interacciones humano animal. En la segunda parte, se revisan los efectos para los humanos de la interacción con animales, especialmente con sus mascotas y se analiza la literatura científica sobre los efectos físicos, psicológicos y sociales. Finalmente, se aborda brevemente el desarrollo de esta área de investigación en Latinoamérica.

  4. Identification and characterization of a novel adenovirus in the cloacal bursa of gulls

    International Nuclear Information System (INIS)

    Bodewes, R.; Bildt, M.W.G. van de; Schapendonk, C.M.E.; Leeuwen, M. van; Boheemen, S. van; Jong, A.A.W. de; Osterhaus, A.D.M.E.; Smits, S.L.; Kuiken, T.

    2013-01-01

    Several viruses of the family of Adenoviridae are associated with disease in birds. Here we report the detection of a novel adenovirus in the cloacal bursa of herring gulls (Larus argentatus) and lesser black-backed gulls (Larus fuscus) that were found dead in the Netherlands in 2001. Histopathological analysis of the cloacal bursa revealed cytomegaly and karyomegaly with basophilic intranuclear inclusions typical for adenovirus infection. The presence of an adenovirus was confirmed by electron microscopy. By random PCR in combination with deep sequencing, sequences were detected that had the best hit with known adenoviruses. Phylogenetic analysis of complete coding sequences of the hexon, penton and polymerase genes indicates that this novel virus, tentatively named Gull adenovirus, belongs to the genus Aviadenovirus. The present study demonstrates that birds of the Laridae family are infected by family-specific adenoviruses that differ from known adenoviruses in other bird species. - Highlights: ► Lesions typical for adenovirus infection detected in cloacal bursa of dead gulls. ► Confirmation of adenovirus infection by electron microscopy and deep sequencing. ► Sequence analysis indicates that it is a novel adenovirus in the genus Aviadenovirus. ► The novel (Gull) adenovirus was detected in multiple organs of two species of gulls

  5. La pobreza energética y sus implicaciones

    OpenAIRE

    Mikel González-Eguino

    2014-01-01

    32 p. El sector energético se enfrenta en las próximas décadas a tres grandes transformaciones relacionadas con el cambio climático, la seguridad de suministro y la pobreza energética. Las dos primeras han sido analizadas ampliamente. Sin embargo, la pobreza energética ha pasado más desapercibida a pesar de tener una gran influencia en la vida de millones de personas, especialmente en los países más pobres. Actualmente, 1.300 millones de personas (el 20% de la población mundial) no tienen ...

  6. Adenovirus-derived vectors for prostate cancer gene therapy

    Czech Academy of Sciences Publication Activity Database

    de Vrij, J.; Willemsen, R. A.; Lindholm, L.; Hoeben, R. C.; Bangma, Ch. H.; Barber, Ch.; Behr, J.-P.; Briggs, S.; Carlisle, R.; Cheng, W.-S.; Dautzenberg, I. J. C.; de Ridder, C.; Dzojic, H.; Erbacher, P.; Essand, M.; Fisher, K.; Frazier, A.; Georgopoulos, L. J.; Jennings, I.; Kochanek, S.; Koppers-Lalic, D.; Kraaij, R.; Kreppel, F.; Magnusson, M.; Maitland, N.; Neuberg, P.; Nugent, R.; Ogris, M.; Remy, J.-S.; Scaife, M.; Schenk, E.; Schooten, E.; Seymour, L.; Slade, M.; Szyjanowicz, P.; Totterman, T.; Uil, T. G.; Ulbrich, Karel; van der Weel, L.; van Weerden, W.; Wagner, E.; Zuber, G.

    2010-01-01

    Roč. 21, č. 7 (2010), s. 795-805 ISSN 1043-0342 EU Projects: European Commission(XE) 512087 - GIANT Keywords : adenovirus * gene delivery * prostate cancer Subject RIV: CD - Macromolecular Chemistry Impact factor: 4.829, year: 2010

  7. Adenovirus Infection in Children with Diarrhea Disease in ...

    African Journals Online (AJOL)

    ANNALS

    Adenovirus Infection in Children with Diarrhea Disease in Northwestern. Nigeria. M. Aminu1, A. A. Ahmad1, J. U. Umoh2, M. C. de Beer3, M. D. Esona3, A. D. Steele3. 1Department of Microbiology, Faculty of Science, Ahmadu Bello University, Zaria Nigeria. 2Department of Veterinary Public Health and Preventive Medicine, ...

  8. characterisation of gastro- enteritis-associated adenoviruses in ...

    African Journals Online (AJOL)

    Objective. To analyse adenovirus (Ad) numbers and types associated with paediatric gastro-enteritis in South Africa. Setting. Gauteng, 1994-1996. Metfwds. A total of 234 paediatric diarrhoeal stool samples were screened for Ad using commercial enzyme-linked. iInmunosorbent assays (EUSAs). Adenoviral isolates were.

  9. Bioaccumulation of animal adenoviruses in the pink shrimp

    Directory of Open Access Journals (Sweden)

    Roger B. Luz

    2015-09-01

    Full Text Available Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100 Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR in order to detect and quantify viral genomes. Thirty-five percent of shrimp samples were positive for contamination, predominantly by avian adenoviruses. A total of 91.7% of water samples contained adenoviruses DNA, with the human form being the most frequent. Our results provided evidence of significant bioaccumulation of adenoviruses in shrimp, showing the extent of the impact of fecal pollution on aquatic ecosystems.

  10. Prevalence of rotavirus, adenovirus and astrovirus infection in young ...

    African Journals Online (AJOL)

    Objective: To determine the prevalence of three enteric viruses, namely rotavirus, adenovirus and astrovirus, as agents of diarrhoea in and around Gaborone, Botswana. Design: The sample were categorised into four groups according to the age of the patient: 0-3 months, 4-6 months, 7-12 months and 25-60 months.

  11. Improvement of oncolytic adenovirus vectors through genetic capsid modifications

    NARCIS (Netherlands)

    Vrij, Jeroen de

    2012-01-01

    Recombinant viral vectors hold great promise in the field of cancer gene therapy. While a plethora of viruses is being evaluated as oncolytic agents, human adenoviruses of serotype 5 (HAdV-5) are among the most popular of viruses to be developed. Although clinical studies have demonstrated safety of

  12. Human erythrocytes bind and inactivate type 5 adenovirus by presenting Coxsackie virus-adenovirus receptor and complement receptor 1

    Czech Academy of Sciences Publication Activity Database

    Carlisle, R. C.; Di, Y.; Cerny, A. M.; Sonnen, A. F. P.; Sim, R. B.; Green, N. K.; Šubr, Vladimír; Ulbrich, Karel; Gilbert, R. J. C.; Fisher, K. D.; Finberg, R. W.; Seymour, L. W.

    2009-01-01

    Roč. 113, č. 9 (2009), s. 1909-1918 ISSN 0006-4971 EU Projects: European Commission(XE) 512087 - GIANT Institutional research plan: CEZ:AV0Z40500505 Keywords : adenovirus * erythrocyte * complement receptor 1 Subject RIV: CD - Macromolecular Chemistry Impact factor: 10.555, year: 2009

  13. [Adenovirus-mediated canine interferon-gamma expression and its antiviral activity against canine parvovirus].

    Science.gov (United States)

    Zhang, Kao; Jin, Huijun; Zhong, Fei; Li, Xiujin; Neng, Changai; Chen, Huihui; Li, Wenyan; Wen, Jiexia

    2012-11-04

    To construct recombinant adenovirus containing canine interferon-gamma (cIFN-gamma) gene and to investigate its antiviral activity against canine parvovirus in Madin-Darby canine kidney cells (MDCK). [Methods] The cIFN-gamma gene was inserted into adenovirus shuttle plasmid to construct pShuttle3-cIFN-gamma expression vector, from which the cIFN-gamma expression cassette was transferred into the adenovirus genomic plasmid pAdeno-X by specific restriction sites to generate recombinant adenovirus genomic plasmid pAd-cIFN-gamma. The pAd-cIFN-gamma plasmid was linearized by digestion and transfected into human embryonic kidney (HEK) 293T cells to generate the replication-defective cIFN-gamma recombinant adenovirus (Ad-cIFN-gamma). To analyze its anti-canine parvovirus activity, the MDCK cells were pre-infected by Ad-cIFN-gamma recombinant adenovirus, and then infected by canine parvovirus. The antiviral activity of the Ad-cIFN-gamma recombinant adenovirus against parvovirus was analyzed. The recombinant adenovirus containing cIFN-gamma gene was constructed by the ligation method. The recombinant adenovirus could mediates recombinant cIFN-gamma secretory expression in MDCK cells. The Ad-cIFN-gamma recombinant adenovirus could significantly inhibit canine parvovirus replication in MDCK cells pre-infected with the recombinant adenovirus. These results indicate that the Ad-cIFN-gamma recombinant adenovirus has the potent antiviral activity against canine parvovirus. The Ad-cIFN-gamma recombinant adenovirus was successfully constructed by the ligation method and possessed a powerful antiviral activity against canine parvovirus.

  14. EL SOFTWARE LIBRE Y SUS IMPLICACIONES JURÍDICAS

    Directory of Open Access Journals (Sweden)

    Gladys Stella Rodríguez

    2008-01-01

    (hardware, software, conocimientos, entre otros. Sólo que esta práctica se ha desnaturalizado creando riesgos de mantenerse el conocimiento y su acceso a un grupo selecto hegemónico. En consecuencia, hoy existe la posibilidad de que a través de licencias públicas generales se consagren derechos a los autores, en un marco de mayor flexibilidad (copyleft. La metodología es descriptiva - explicativa, con una revisión bibliográfica y hemerográficas sobre el tema. Asimismo, se consultó a Fundación para la Ciencia y la Tecnología (FUNDACITE ZULIA, a través de la Misión Ciencia. Se concluye que el software libre es una oportunidad para democratizar el acceso a la información en países en desarrollo, y es un reto para el desarrollador de softwares.

  15. Estilo de pensamiento del alumnado y profesorado universitario. Implicaciones educativas

    Directory of Open Access Journals (Sweden)

    Pedro ALLUEVA TORRES

    2010-01-01

    Full Text Available En el estudio se tomaron datos del alumnado de 1º curso, egresado y profesorado de 1º curso de la Universidad de Zaragoza en el curso 2008-2009, con el fin de analizar los diferentes perfiles de estilos de pensamiento predominantes para poder determinar qué metodologías y sistemas de evaluación en el proceso de enseñanza- aprendizaje pueden favorecer el aprendizaje y desarrollo de habilidades y aptitudes del pensamiento en el alumnado. Al realizar el análisis de los datos por grupos, áreas de conocimiento y sexo, se aprecian diferencias significativas en los perfiles de los estilos de pensamiento de los participantes.

  16. e implicaciones de política pública

    Directory of Open Access Journals (Sweden)

    Luis Miguel Galindo

    2006-01-01

    Full Text Available El principal objetivo de este estudio es identificar la existencia del fenómeno del tráfico inducido en México. Los resultados obtenidos indican que la ampliación de las vialidades en México origina un aumento de los viajes y de los kilómetros recorridos como consecuencia de una disminución del tiempo de traslado. Esto es, la demanda de viajes puede modelarse como una función de demanda tradicional en donde una disminución de los costos por transporte, que incluyen tanto costos monetarios directos como de tiempo, se traduce en un aumento de la cantidad o longitud de los viajes. Las estimaciones realizadas indican que la elasticidad de las vialidades al consumo de gasolina es de 0.15, confirmando la presencia del tráfico inducido. Asimismo se presentan algunos ejercicios preliminares sobre posibles estimaciones del tráfico inducido para vialidades específicas, aunque debe reconocerse que los valores estimados para el país no necesariamente se aplican al caso de la Ciudad de México. El conjunto de resultados obtenidos indica que la ampliación de la infraestructura vial viene asociada a un aumento menos que proporcional del tráfico, pues se disminuye momentáneamente la razón de uso de las vialidades que, paulatinamente, se va incrementando. De este modo la ampliación vial genera en el corto plazo una mejora en el servicio que tiende a deteriorarse con el tiempo. Esta mejora en el servicio debe sin embargo evaluarse con referencia a los costos que implica en el largo plazo una opción de desarrollo urbano que privilegia el uso del automóvil como medio de transporte en contraposición a otras alternativas.

  17. La suavidad de la carne: implicaciones físicas y bioquímicas asociadas al manejo y proceso agroindustrial

    Directory of Open Access Journals (Sweden)

    Alejandro Chac\\u00F3n

    2004-01-01

    Full Text Available La suavidad de la carne: implicaciones físicas y bioquímicas asociadas al manejo y proceso agroindustrial. Actualmente, el problema de la suavidad de la carne representa una gran preocupación entre los productores, dado que esta variable ha demostrado ser el principal criterio con base al cual los consumidores juzgan la calidad de la carne. El problema es especialmente importante si se considera que una de cada cuatro degustaciones de la carne resultan insatisfactorias para el consumidor. Este trabajo abordó los principales aspectos asociados con la suavidad de la carne tales como la estructura y composición de la carne, el fenómeno de la contracción muscular, los cambios bioquímicos post mortem como el rigor mortis y la maduración, y el efecto general debido a las operaciones del procesamiento industrial como la cocción y el congelamiento. Métodos generales para el mejoramiento de la suavidad son discutidos, así como la actividad bioquímica e importancia de las enzimas CALPAINAS (calcium ion dependent papain like cisteine proteases.

  18. Desarrollo de proveedores locales en el sector extractivo colombiano: cuellos de botella, factores de éxito e implicaciones de política pública

    Directory of Open Access Journals (Sweden)

    Juan David Gutiérrez Rodríguez

    2015-12-01

    Full Text Available Las sociedades que cuentan con abundantes recursos naturales no renovables pueden transformar dicho capital natural en otros tipos de capital (p. ej. humano, producido, infraestructura a través de diferentes canales. Para los gobiernos que deben administrar la riqueza de los recursos naturales, la sustitución de un tipo de capital –que se agota a medida que se explota– por otros capitales sostenibles, es un reto de política pública. Uno de los canales de transformación lo constituye la creación de encadenamientos productivos –“hacia adelante” y “hacia atrás”– entre el sector extractivo y otros sectores económicos. Este artículo tiene dos objetivos: i identificar los principales cuellos de botella y factores de éxito para el desarrollo de proveedores nacionales y locales para el sector extractivo colombiano y ii las implicaciones de política pública relacionadas con la promoción de encadenamientos productivos entre los sectores mineros y de hidrocarburos, y otros sectores en Colombia.

  19. Culturas juveniles y trabajo social con jóvenes

    Directory of Open Access Journals (Sweden)

    GLADYS CASTIBLANCO-LEMUS

    2008-01-01

    Full Text Available Este trabajo tiene como propósito señalar algunas implicaciones teóricas y practicas de la relación entre la categoría culturas juveniles, concretamente desde los resultados del proyecto de investigación: Identidades juveniles: Música y producción cultural. Etnografía de agrupaciones Rave y hip hop rap en Bogotá y un conjunto de premisas conceptuales del trabajo social con jóvenes. Para tal efecto se abordarán tres aspectos: en primer lugar, se presenta una síntesis del proyecto destacando los más importantes resultados; en segundo lugar se plantean los referentes conceptuales que permiten establecer la relación y finalmente se señalan algunas pistas para incorporar otros contenidos para la actuación profesional con jóvenes.

  20. Implicaciones de la Intoxicación por Mercurio.

    Directory of Open Access Journals (Sweden)

    Myriam Gutiérrez de Salazar

    2004-06-01

    Full Text Available

    Generalidades

    El mercurio puede encontrarse en diversidad de minerales, yacimientos y en una gran variedad de estados físicos y químicos, cada uno de ellos tiene toxicidad diferente y sus aplicaciones en la industria, la agricultura y la medicina requiere de distintas evaluaciones. En Colombia es de gran importancia su estudio por el uso indiscriminado que se hace del mercurio metálico durante los procesos de “amalgamación” del oro durante la actividad minera de extracción de oro en varias zonas de nuestro país, constituyendo un riesgo tóxico para los seres humanos y todos los ecosistemas terrestres y acuáticos. Estados del mercurio

    • Mercurio metálico o elemental, es usado en la extracción de oro y plata, en amalgamas dentales y en una gran variedad de elementos de medición de temperatura y presión en medicina y en la industria.

    • Mercurio inorgánico o Sales de Mercurio son utilizadas como antiséptico en quemaduras, y fueron alguna vez utilizadas como diuréticos.

    • Organomercuriales, han sido utilizados como fungicidas y antisépticos. El más importante desde el punto de vista tóxico es el metilmercurio, el cual puede acumularse en las especies acuáticas debido a la contaminación ambiental. En los años cincuenta una planta química cercana a la Bahía de Minamata (Japón descargó alta cantidad de residuos mercuriales en el mar, lo cual contaminó el plancton y el pescado que fue consumido posteriormente por los habitantes locales, originando una intoxicación masiva de características catastróficas, con 121 afectados, 46 muertos y muchos niños con daños severos en el sistema nervioso central...

  1. Implicaciones sociales de los sistemas de computadoras telecomunicaciones

    Directory of Open Access Journals (Sweden)

    Edwin Parker

    2015-05-01

    Full Text Available La crisis económica de mediados de la década del 70 es un síntoma de una transición social importante. La causa principal es el cambio de las sociedades industrializadas a sociedades post-industrializadas y la introducción resultante, de elementos post-industriales en los procesos de industrialización en los países menos desarrollados. Estamos en medio de una transición, de una sociedad industrial a una sociedad de información; se está ingresando en una era en la cual la actividad predominante es el procesamiento de la información y no la producción industrial. El adelanto de las computadoras, las comunicaciones y otras técnicas de información en los últimos 25 años, ha sido llamado, con razón, "la segunda revolución industrial".

  2. Nucleotide sequence analysis of regions of adenovirus 5 DNA containing the origins of DNA replication

    International Nuclear Information System (INIS)

    Steenbergh, P.H.

    1979-01-01

    The purpose of the investigations described is the determination of nucleotide sequences at the molecular ends of the linear adenovirus type 5 DNA. Knowledge of the primary structure at the termini of this DNA molecule is of particular interest in the study of the mechanism of replication of adenovirus DNA. The initiation- and termination sites of adenovirus DNA replication are located at the ends of the DNA molecule. (Auth.)

  3. Early RNA of adenovirus type 3 in permissive and abortive infections.

    OpenAIRE

    Groff, D E; Daniell, E

    1981-01-01

    Early adenovirus type 3 cytoplasmic polyadenylated RNAs from HeLa and BHK-21 cells were detected and mapped on the viral genome by gel blotting and hybridization techniques. The sizes and locations of the 16 adenovirus type 3 RNAs were identical in the two cell types, although relative molarities of the various RNA species differed. Each of the early adenovirus type 3 RNAs was associated with polysomes in both cell types, suggesting that the abortive infection of hamster cells does not result...

  4. Showing the Way: Oncolytic Adenoviruses as Chaperones of Immunostimulatory Adjuncts

    Directory of Open Access Journals (Sweden)

    Jing Li Huang

    2016-09-01

    Full Text Available Oncolytic adenoviruses (OAds are increasingly recognized as vectors for immunotherapy in the treatment of various solid tumors. The myriads of advantages of using adenovirus include targeted specificity upon infection and selective replication, which lead to localized viral burst, exponential spread of OAds, and antitumor effect. OAds can also induce a strong immune reaction due to the massive release of tumor antigens upon cytolysis and the presence of viral antigens. This review will highlight recent advances in adenoviral vectors expressing immunostimulatory effectors, such as GM-CSF (granulocyte macrophage colony-stimulating factor, interferon-α, interleukin-12, and CD40L. We will also discuss the combination of OAds with other immunotherapeutic strategies and describe the current understanding of how adenoviral vectors interact with the immune system to eliminate cancer cells.

  5. Showing the Way: Oncolytic Adenoviruses as Chaperones of Immunostimulatory Adjuncts.

    Science.gov (United States)

    Huang, Jing Li; LaRocca, Christopher J; Yamamoto, Masato

    2016-09-19

    Oncolytic adenoviruses (OAds) are increasingly recognized as vectors for immunotherapy in the treatment of various solid tumors. The myriads of advantages of using adenovirus include targeted specificity upon infection and selective replication, which lead to localized viral burst, exponential spread of OAds, and antitumor effect. OAds can also induce a strong immune reaction due to the massive release of tumor antigens upon cytolysis and the presence of viral antigens. This review will highlight recent advances in adenoviral vectors expressing immunostimulatory effectors, such as GM-CSF (granulocyte macrophage colony-stimulating factor), interferon-α, interleukin-12, and CD40L. We will also discuss the combination of OAds with other immunotherapeutic strategies and describe the current understanding of how adenoviral vectors interact with the immune system to eliminate cancer cells.

  6. Dielectrophoresis and dielectrophoretic impedance detection of adenovirus and rotavirus

    Science.gov (United States)

    Nakano, Michihiko; Ding, Zhenhao; Suehiro, Junya

    2016-01-01

    The aim of this study is the electrical detection of pathogenic viruses, namely, adenovirus and rotavirus, using dielectrophoretic impedance measurement (DEPIM). DEPIM consists of two simultaneous processes: dielectrophoretic trapping of the target and measurement of the impedance change and increase in conductance with the number of trapped targets. This is the first study of applying DEPIM, which was originally developed to detect bacteria suspended in aqueous solutions, to virus detection. The dielectric properties of the viruses were also investigated in terms of their dielectrophoretic behavior. Although their estimated dielectric properties were different from those of bacteria, the trapped viruses increased the conductance of the microelectrode in a manner similar to that in bacteria detection. We demonstrated the electrical detection of viruses within 60 s at concentrations as low as 70 ng/ml for adenovirus and 50 ng/ml for rotavirus.

  7. Adenovirus type 5 induces progression of quiescent rat cells into S phase without polyamine accumulation.

    Science.gov (United States)

    Cheetham, B F; Shaw, D C; Bellett, A J

    1982-01-01

    Adenovirus type 5 induces cellular DNA synthesis and thymidine kinase in quiescent rat cells but does not induce ornithine decarboxylase. We now show that unlike serum, adenovirus type 5 fails to induce S-adenosylmethionine decarboxylase or polyamine accumulation. The inhibition by methylglyoxal bis(guanylhydrazone) of the induction of thymidine kinase by adenovirus type 5 is probably unrelated to its effects on polyamine biosynthesis. Thus, induction of cellular thymidine kinase and DNA replication by adenovirus type 5 is uncoupled from polyamine accumulation. PMID:7177112

  8. Recent advances in genetic modification of adenovirus vectors for cancer treatment.

    Science.gov (United States)

    Yamamoto, Yuki; Nagasato, Masaki; Yoshida, Teruhiko; Aoki, Kazunori

    2017-05-01

    Adenoviruses are widely used to deliver genes to a variety of cell types and have been used in a number of clinical trials for gene therapy and oncolytic virotherapy. However, several concerns must be addressed for the clinical use of adenovirus vectors. Selective delivery of a therapeutic gene by adenovirus vectors to target cancer is precluded by the widespread distribution of the primary cellular receptors. The systemic administration of adenoviruses results in hepatic tropism independent of the primary receptors. Adenoviruses induce strong innate and acquired immunity in vivo. Furthermore, several modifications to these vectors are necessary to enhance their oncolytic activity and ensure patient safety. As such, the adenovirus genome has been engineered to overcome these problems. The first part of the present review outlines recent progress in the genetic modification of adenovirus vectors for cancer treatment. In addition, several groups have recently developed cancer-targeting adenovirus vectors by using libraries that display random peptides on a fiber knob. Pancreatic cancer-targeting sequences have been isolated, and these oncolytic vectors have been shown by our group to be associated with a higher gene transduction efficiency and more potent oncolytic activity in cell lines, murine models, and surgical specimens of pancreatic cancer. In the second part of this review, we explain that combining cancer-targeting strategies can be a promising approach to increase the clinical usefulness of oncolytic adenovirus vectors. © 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

  9. ENTERIC ADENOVIRUS INFECTION IN INFANTS AND YOUNG CHILDREN WITH ACUTE GASTROENTERITIS IN TEHRAN

    Directory of Open Access Journals (Sweden)

    F. Jam-Afzon S. Modarres

    2006-09-01

    Full Text Available Adenoviruses are one of the most important etiological agents of serious gastroenteritis among infants and young children. Fecal specimens from patients with an acute gastroenteritis were evaluated for the presence of adenovirus (Ad40, 41 from April 2002 to February 2004. During the study, 1052 samples were collected from children under the age of 5 years in six educational and therapeutic pediatric centers. The specimens were tested for adenovirus (Ad40, 41 by EIA technique in the Virology Department of Pasteur Institute of Iran. Adenoviruses (Ad40, 41 were detected from 27(2.6% samples, but were not detected in 150 samples of healthy control group. In this study the highest rate of adenovirus was found in children aged 6 to 12 months (40.7%, but the male to female ratio inpatients was approximately equal. Adenovirus (Ad40, 41 infections peaked in the winter as 48.1% was detected from December to March. There were a statistically significant difference between age and infection (P < 0.001, also between season with adenovirus (Ad40, 41 infection (P = 0.005. Breast-feeding had a protective action against adenovirus (Ad40, 41 infection. This study revealed that enteric adenovirus (Ad40, 41 is an etiological agent of acute gastroenteritis among children in Tehran.

  10. Concentration of Reovirus and Adenovirus from Sewage and Effluents by Protamine Sulfate (Salmine) Treatment 1

    Science.gov (United States)

    England, Beatrice

    1972-01-01

    Protamine sulfate was employed to recover reoviruses, adenoviruses, and certain enteroviruses from sewage and treated effluents; 50- to 400-fold concentration of viral content was achieved. PMID:4342842

  11. Optimization and evaluation of a method to detect adenoviruses in river water

    Data.gov (United States)

    U.S. Environmental Protection Agency — This dataset includes the recoveries of spiked adenovirus through various stages of experimental optimization procedures. This dataset is associated with the...

  12. Prolonged peritoneal gene expression using a helper-dependent adenovirus.

    Science.gov (United States)

    Liu, Limin; Shi, Chang-Xin; Ghayur, Ayesha; Zhang, Claire; Su, Je Yen; Hoff, Catherine M; Margetts, Peter J

    2009-01-01

    Encapsulating peritoneal sclerosis (EPS) is a rare complication of peritoneal dialysis. The causes of EPS are not well defined and are likely multifactorial. A suitable animal model would facilitate research into the pathophysiology and treatment of EPS. We developed a helper-dependent adenovirus that expresses both green fluorescent protein (GFP) and active transforming growth factor-beta (TGF-beta1; HDAdTGF-beta1). Mice were administered HDAdTGF-beta1 via intraperitoneal injection and the response was compared with mice administered either first-generation adenovirus expressing TGF-beta1 (AdTGF-beta1) or control adenovirus (AdGFP). HDAdTGF-beta1-treated mice continued to express the GFP reporter transgene to day 74, the end of the observation period. Transgene expression lasted less than 28 days in the animals treated with first-generation adenoviruses. Animals treated with first-generation AdTGF-beta1 demonstrated submesothelial thickening and angiogenesis at day 7, with almost complete resolution by day 28. The HDAdTGF-beta1-treated mice demonstrated progressive peritoneal fibrosis with adhesion formation and encapsulation of bowels. Weight gain was significantly reduced in animals treated with HDAdTGF-beta1 compared to both the control-treated animals and the AdTGF-beta1-treated animals. Inflammation was not a major component of the fibroproliferative response. Peritoneal administration of a first-generation AdTGF-beta1 leads to transient gene expression, resulting in a resolving fibrotic response and histology similar to that seen in simple peritoneal sclerosis. Prolonged TGF-beta1 expression induced by the helper-dependent HDAdTGF-beta1 led to changes in peritoneal morphology resembling EPS. This suggests that TGF-beta1 may be a contributing factor in both simple peritoneal sclerosis and EPS. This model will be useful for elucidation of the mechanism of EPS and evaluation of potential treatment.

  13. An Update on Canine Adenovirus Type 2 and Its Vectors

    Science.gov (United States)

    Bru, Thierry; Salinas, Sara; Kremer, Eric J.

    2010-01-01

    Adenovirus vectors have significant potential for long- or short-term gene transfer. Preclinical and clinical studies using human derived adenoviruses (HAd) have demonstrated the feasibility of flexible hybrid vector designs, robust expression and induction of protective immunity. However, clinical use of HAd vectors can, under some conditions, be limited by pre-existing vector immunity. Pre-existing humoral and cellular anti-capsid immunity limits the efficacy and duration of transgene expression and is poorly circumvented by injections of larger doses and immuno-suppressing drugs. This review updates canine adenovirus serotype 2 (CAV-2, also known as CAdV-2) biology and gives an overview of the generation of early region 1 (E1)-deleted to helper-dependent (HD) CAV-2 vectors. We also summarize the essential characteristics concerning their interaction with the anti-HAd memory immune responses in humans, the preferential transduction of neurons, and its high level of retrograde axonal transport in the central and peripheral nervous system. CAV-2 vectors are particularly interesting tools to study the pathophysiology and potential treatment of neurodegenerative diseases, as anti-tumoral and anti-viral vaccines, tracer of synaptic junctions, oncolytic virus and as a platform to generate chimeric vectors. PMID:21994722

  14. Progress on adenovirus-vectored universal influenza vaccines.

    Science.gov (United States)

    Xiang, Kui; Ying, Guan; Yan, Zhou; Shanshan, Yan; Lei, Zhang; Hongjun, Li; Maosheng, Sun

    2015-01-01

    Influenza virus (IFV) infection causes serious health problems and heavy financial burdens each year worldwide. The classical inactivated influenza virus vaccine (IIVV) and live attenuated influenza vaccine (LAIV) must be updated regularly to match the new strains that evolve due to antigenic drift and antigenic shift. However, with the discovery of broadly neutralizing antibodies that recognize conserved antigens, and the CD8(+) T cell responses targeting viral internal proteins nucleoprotein (NP), matrix protein 1 (M1) and polymerase basic 1 (PB1), it is possible to develop a universal influenza vaccine based on the conserved hemagglutinin (HA) stem, NP, and matrix proteins. Recombinant adenovirus (rAd) is an ideal influenza vaccine vector because it has an ideal stability and safety profile, induces balanced humoral and cell-mediated immune responses due to activation of innate immunity, provides 'self-adjuvanting' activity, can mimic natural IFV infection, and confers seamless protection against mucosal pathogens. Moreover, this vector can be developed as a low-cost, rapid-response vaccine that can be quickly manufactured. Therefore, an adenovirus vector encoding conserved influenza antigens holds promise in the development of a universal influenza vaccine. This review will summarize the progress in adenovirus-vectored universal flu vaccines and discuss future novel approaches.

  15. APRENDIENDO A VIVIR CON EL ALUVIÓN INFORMATIVO Y SUS IMPLICACIONES PARA LA EDUCACIÓN

    OpenAIRE

    Jones, Barbara; Goff, Mhoraf

    2011-01-01

    Una característica importante de nuestra época es el aumento exponencial de los datos. Grandes cantidades de información, en formato digital, son consumidas para la investigación, y a la vez derivadas de la misma. Internet ha hecho la "información" accesible -no solo datos- a aquellas personas que tienen los recursos necesarios para acceder y utilizar Internet. Como resultado, se ha generado para las instituciones educativas un nuevo conjunto de requisitos para dotar a sus profesores y alumno...

  16. Seguridad en el mar, algunas implicaciones de los códigos IGS y PBIB.

    OpenAIRE

    José Vicente Guzmán Escobar

    2007-01-01

    La expedición del Código Internacional de Gestión de la Seguridad Marítima (Código IGS) y del Código de Protección de Buques e Instalaciones Portuarias (Código PBIB), en el contexto del Convenio SOLAS, tiene especiales implicaciones legales en diferentes figuras del derecho marítimo internacional, tales como la limitación de responsabilidad de los armadores de buques por reclamaciones marítimas, la limitación de responsabilidad de los armadores por contaminación por derrames de hidrocarburos,...

  17. A rapid Q-PCR titration protocol for adenovirus and helper-dependent adenovirus vectors that produces biologically relevant results

    Science.gov (United States)

    Gallaher, Sean D.; Berk, Arnold J.

    2013-01-01

    Adenoviruses are employed in the study of cellular processes and as expression vectors used in gene therapy. The success and reproducibility of these studies is dependent in part on having accurate and meaningful titers of replication competent and helper-dependent adenovirus stocks, which is problematic due to the use of varied and divergent titration protocols. Physical titration methods, which quantify the total number of viral particles, are used by many, but are poor at estimating activity. Biological titration methods, such as plaque assays, are more biologically relevant, but are time consuming and not applicable to helper-dependent gene therapy vectors. To address this, a protocol was developed called “infectious genome titration” in which viral DNA is isolated from the nuclei of cells ~3 h post-infection, and then quantified by Q-PCR. This approach ensures that only biologically active virions are counted as part of the titer determination. This approach is rapid, robust, sensitive, reproducible, and applicable to all forms of adenovirus. Unlike other Q-PCR-based methods, titers determined by this protocol are well correlated with biological activity. PMID:23624118

  18. Relaciones sexuales en adolescentes colombianos y las implicaciones para la salud pública: una revisión de la prevalencia y algunas variables asociadas

    Directory of Open Access Journals (Sweden)

    Adalberto Campo-Arias

    2009-08-01

    Full Text Available ResumenEl deseo sexual se incrementa significativamente durante la adolescencia, en consecuencia, un número importante de hombres y mujeres adolescentes inician relaciones sexuales antes de la mayoría de edad. Se realizó una revisión de las publicaciones en Pubmed y Google académico que informaban la prevalencia de relaciones sexuales en adolescentes colombianos durante la década más reciente (1999-2008. Las palabras clave que se usaron fueron adolescentes (adolescents, estudiantes (students, comportamiento sexual (sexual behavior y Colombia. La prevalencia de relaciones sexuales estuvo entre 7.7% y 58%, media de 27.2%. Más años de edad, sexo masculino, mayor escolaridad y consumo de sustancias legales o ilegales se relacionaron con historia de relaciones sexuales. Los adolescentes informaron conductas sexuales de riesgo, con diferencias por género, como el uso inconsistente de condón, relaciones sexuales bajo el efecto de sustancias y relaciones sexuales con personas poco conocidas. Estos hallazgos tienen relevancia para salud pública sexual y reproductiva. Se deben diseñar programas integrales de intervención para promover un desarrollo sexual armónico y saludable en adolescentes colombianos. [Campo-Arias A. Relaciones sexuales en adolescentes colombianos y las implicaciones para la salud pública: una revisión de la prevalencia y algunas variables asociadas. MedUNAB 2009; 12:86-90].

  19. Oncolytic adenovirus Ad657 for systemic virotherapy against prostate cancer

    Directory of Open Access Journals (Sweden)

    Nguyen TV

    2018-05-01

    Full Text Available Tien V Nguyen,1,* Catherine M Crosby,2,* Gregory J Heller,3 Zachary I Mendel,3 Mary E Barry,1 Michael A Barry1,4,5 1Department of Internal Medicine, Division of Infectious Diseases, 2Virology and Gene Therapy Graduate Program, Mayo Clinic Graduate School of Biomedical Sciences, 3Postbaccalaureate Research Education Program, Mayo Clinic Graduate School of Biomedical Sciences, 4Department of Immunology, 5Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA *These authors contributed equally to this work Background: Human species C adenovirus serotype 5 (Ad5 is the archetype oncolytic adenovirus and has been used in the vast majority of preclinical and clinical tests. While Ad5 can be robust, species C Ad6 has lower seroprevalence, side effects, and appears to be more potent as a systemic therapy against a number of tumors than Ad5. Historically, there have only been four species C human adenoviruses: serotypes 1, 2, 5, and 6. More recently a new species C adenovirus, Ad57, was identified. Ad57 is most similar to Ad6 with virtually all variation in their capsid proteins occurring in the hypervariable regions (HVRs of their hexon proteins. Most adenovirus neutralizing antibodies target the HVRs on adenoviruses. This led us to replace the hexon HVRs in Ad6 with those from Ad57 to create a new virus called Ad657 and explore this novel species C platform’s utility as an oncolytic virus. Methods: The HVR region from Ad57 was synthesized and used to replace the Ad6 HVR region by homologous recombination in bacteria generating a new viral platform that we call Ad657. Replication-competent Ad5, Ad6, and Ad657 were compared in vitro and in vivo for liver damage and oncolytic efficacy against prostate cancers after single intravenous treatment in mice. Results: Ad5, Ad6, and Ad657 had similar in vitro oncolytic activity against human prostate cancer cells. Ad5 provoked the highest level of liver toxicity after intravenous injection and Ad657

  20. Desafíos de la gobernanza ambiental: una aproximación a las implicaciones de la Gestión Integrada del Recurso Hídrico en Colombia

    Directory of Open Access Journals (Sweden)

    Nicolás González

    2017-01-01

    Full Text Available En búsqueda de realizar una aproximación a las implicaciones de la gobernanza del agua en Colombia, este trabajo (1 indaga acerca del concepto en cuestión, (2 su relación con la Gestión Integrada del Recurso Hídrico (GIRH, (3 expone brevemente el marco institucional que soporta el patrón de gestión de aguas vigente en Colombia, (4 lo analiza críticamente y (5 finalmente, presenta algunas consideraciones pertinentes a cerca de las posibilidades y desafíos que, desde las instituciones, supone la implementación de modelos de GIRH en Colombia.

  1. ¿Fue Wittgenstein un conductista? La necesidad de criterios públicos para el juego de lenguaje de lo ?interno? y sus implicaciones para la Psicología

    Directory of Open Access Journals (Sweden)

    Antonio Crego Díaz

    2004-11-01

    Full Text Available Se argumenta a favor de una interpretación conductista de Wittgenstein, caracterizando esta postura como ?conductismo epistemológico? ?referido a juegos de lenguaje-, que no niega ?en el plano ontológico- la existencia de lo mental, que interpreta la conducta en sentido amplio y que tiene un marcado carácter social. En la base de este conductismo se encuentra la idea de que para establecer el juego de lenguaje de lo interno se precisa de criterios públicos, intersubjetivos. Se explora la posibilidad de elaborar una epistemología sociopsicológica naturalizada a partir del análisis lingüístico skinneriano y en consonancia con las observaciones de Wittgenstein. Se comentan las implicaciones del conductismo epistemológico para el lenguaje de los términos psicológicos.

  2. El interrogante de lo vivo y sus respuestas ahistóricas: desarrollo histórico del concepto de lo vivo y algunas implicaciones didácticas.

    Directory of Open Access Journals (Sweden)

    Mónica Correa (Página 153-163

    2009-07-01

    Full Text Available El  presente trabajo muestra  el desarrollo histórico y epistemológico del concepto de lo vivo, en términos de las diferentes corrientes de pensamiento que han guiado su estudio, como son: El animismo, el mecanicismo, el vitalismo, el organicismo, el pensamiento sistémico y el neomecanicismo. Además, relacionando lo  anterior con  la formación docente y buscando una reflexión acerca de la importancia de vincular la historia y la epistemología en la enseñanza de las ciencias, se hace una revisión de algunas  implicaciones  didácticas del abordaje ahistórico de de este concepto en la escuela.

  3. Respiratory adenovirus-like infection in a rose-ringed parakeet (Psittacula krameri).

    Science.gov (United States)

    Desmidt, M; Ducatelle, R; Uyttebroek, E; Charlier, G; Hoorens, J

    1991-01-01

    Intranuclear inclusions were observed under light microscopy in the bronchial epithelial cells of a recently purchased female rose-ringed parakeet that died of chlamydiosis. Transmission electron microscopy revealed the presence of numerous particles of adenovirus morphology. A latent adenovirus infection may have become more severe following chlamydiosis and the stress of handling.

  4. Interspecies differences in virus uptake versus cardiac function of the coxsackievirus and adenovirus receptor.

    NARCIS (Netherlands)

    Freiberg, F.; Sauter, M.; Pinkert, S.; Govindarajan, T.; Kaldrack, J.; Thakkar, M.; Fechner, H.; Klingel, K.; Gotthardt, M.

    2014-01-01

    The coxsackievirus and adenovirus receptor (CAR) is a cell contact protein with an important role in virus uptake. Its extracellular immunoglobulin domains mediate the binding to coxsackievirus and adenovirus as well as homophilic and heterophilic interactions between cells. The cytoplasmic tail

  5. Comparative inactivation of enteric adenoviruses, poliovirus and coliphages by ultraviolet irradiation

    International Nuclear Information System (INIS)

    Meng, Q.S.; Gerba, C.P.

    1996-01-01

    The inactivation of enteric adenoviruses 40 and 41 by ultraviolet (UV) radiation was investigated and compared with poliovirus type 1 (strain LSc-2ab) and coliphages MS-2 and PRD-1. Purified stocks of the viruses were exposed to collimated ultraviolet radiation in a stirred reactor for a total dose of up to 140 mW s/cm 2 . The doses of UV to achieve a 90% inactivation of adenovirus 40, adenovirus 41, coliphages MS-2 and PRD-1 and poliovirus type 1 were 30, 23.6, 14, 8.7 and 4.1 mW s/cm 2 , respectively. Adenovirus 40 was significantly more resistant than coliphage MS-2 to UV irradiation (P < 0.01). Adenovirus 41 appeared slightly more sensitive than adenovirus 40, but the difference was not significant (P>0.05). The resistance of PRD-1 was less than MS-2 (P < 0.01), but greater than poliovirus type 1 (P < 0.01). Adenoviruses 40 and 41 were more resistant than Bacillus subtilis spores, often suggested as an indicator of UV light performance. The double-stranded DNA adenoviruses appear to be the most resistant of all potentially water-borne enteric viruses to UV light disinfection. (author)

  6. A simple negative selection method to identify adenovirus recombinants using colony PCR

    Directory of Open Access Journals (Sweden)

    Yongliang Zhao

    2014-01-01

    Conclusions: The negative selection method to identify AdEasy adenovirus recombinants by colony PCR can identify the recombined colony within a short time-period, and maximally avoid damage to the recombinant plasmid by limiting recombination time, resulting in improved adenovirus packaging.

  7. Crystal structure of the fibre head domain of the Atadenovirus Snake Adenovirus 1.

    Directory of Open Access Journals (Sweden)

    Abhimanyu K Singh

    Full Text Available Adenoviruses are non-enveloped icosahedral viruses with trimeric fibre proteins protruding from their vertices. There are five known genera, from which only Mastadenoviruses have been widely studied. Apart from studying adenovirus as a biological model system and with a view to prevent or combat viral infection, there is a major interest in using adenovirus for vaccination, cancer therapy and gene therapy purposes. Adenoviruses from the Atadenovirus genus have been isolated from squamate reptile hosts, ruminants and birds and have a characteristic gene organization and capsid morphology. The carboxy-terminal virus-distal fibre head domains are likely responsible for primary receptor recognition. We determined the high-resolution crystal structure of the Snake Adenovirus 1 (SnAdV-1 fibre head using the multi-wavelength anomalous dispersion (MAD method. Despite the absence of significant sequence homology, this Atadenovirus fibre head has the same beta-sandwich propeller topology as other adenovirus fibre heads. However, it is about half the size, mainly due to much shorter loops connecting the beta-strands. The detailed structure of the SnAdV-1 fibre head and other animal adenovirus fibre heads, together with the future identification of their natural receptors, may lead to the development of new strategies to target adenovirus vectors to cells of interest.

  8. A novel technology to target adenovirus vectors : application in cells involved in atherosclerosis

    NARCIS (Netherlands)

    Gras, Jan Cornelis Emile

    2007-01-01

    In this thesis a novel technology is described to target adenovirus vectors. Adenovirus vectors are powerful tools to modulate gene expression. The use of these vectors however, is hampered by the fact that many for gene therapy interesting cell types do not, or only at low levels express the CAR

  9. Presence of adenovirus species C in infiltrating lymphocytes of human sarcoma.

    Directory of Open Access Journals (Sweden)

    Karin Kosulin

    Full Text Available Human adenoviruses are known to persist in T-lymphocytes of tonsils, adenoids and intestinal tract. The oncogenic potential of different adenovirus types has been widely studied in rodents, in which adenovirus inoculation can induce multiple tumors such as undifferentiated sarcomas, adenocarcinomas and neuroectodermal tumors. However, the oncogenic potential of this virus has never been proven in human subjects. Using a highly sensitive broad-spectrum qRT-PCR, we have screened a set of different human sarcomas including leiomyosarcoma, liposarcoma and gastro intestinal stroma tumors. Primers binding the viral oncogene E1A and the capsid-coding gene Hexon were used to detect the presence of adenovirus DNA in tumor samples. We found that 18% of the tested leiomyosarcomas and 35% of the liposarcomas were positive for the presence of adenovirus DNA, being species C types the most frequently detected adenoviruses. However, only in one sample of the gastro intestinal stroma tumors the virus DNA could be detected. The occurrence of adenovirus in the tumor sections was confirmed by subsequent fluorescence in-situ-hybridization analysis and co-staining with the transcription factor Bcl11b gives evidence for the presence of the virus in infiltrating T-lymphocytes within the tumors. Together these data underline, for the first time, the persistence of adenovirus in T-lymphocytes infiltrated in muscular and fatty tissue tumor samples. If an impaired immune system leads to the viral persistence and reactivation of the virus is involved in additional diseases needs further investigation.

  10. Tendencias de especialización maquiladora: implicaciones de la configuración socio-técnica en Nogales, Sonora

    Directory of Open Access Journals (Sweden)

    Araceli Almaraz Alvarado

    1998-01-01

    Full Text Available En este trabajo se discute la posibilidad de incluir en el análisis de la industria maquiladora de exportación (I M E , las implicaciones de la movilidad espacial de las empresas en el proceso de especialización. Partiendo de la importancia del proceso de configuración socio-técnica en la I M E, se busca identificar su correspondencia con el proceso de interacción y expansión de las plantas, a través de las particularidades locales y diferencias entre los tipos de firmas de la I M E concentrada en la ciudad de Nogales, Sonora. A lo largo del artículo se desarrolla la idea de que debe existir una diferenciación entre localidades y / o regiones donde la IME se ha venido desenvolviendo, la cual, finalmente, deberá responder a la urgencia de presentar tipologías de trayectorias industriales que reflejen las tendencias de organización de la producción local y regional, y sus conexiones al ámbito de las cadenas globales.

  11. La hipótesis del trauma en el abuso sexual: revisión crítica e implicaciones

    Directory of Open Access Journals (Sweden)

    Agustín Malón Marco

    2008-01-01

    Full Text Available Se lleva a cabo una revisión crítica de la hipótesis traumática en el estudio de las experiencias eróticas tempranas con personas más mayores. Esta hipótesis, la más habitual en la literatura científica, consiste en dar por cierto que son en su mayoría experiencias graves y de serias consecuencias negativas a corto y/o largo plazo. Cuestionaremos su fundamentación empírica, para después pasar a las implicaciones científicas y profesionales surgidas de otra lectura de los datos disponibles. Para ello se analizan críticamente nueve revisiones previas sobre la cuestión, publicadas entre 1981 y 1998. Esta revisión de revisiones sugiere que estaría perfectamente sustentada la hipótesis de que en la gran mayoría de los casos no suelen producirse daños serios ni mucho menos permanentes en los implicados. El análisis invita a un replanteamiento teórico, epistemológico y tecnológico de la investigación y la intervención social.

  12. FUNCIONES EJECUTIVAS EN NIÑOS Y ADOLESCENTES: IMPLICACIONES DEL TIPO DE MEDIDAS DE EVALUACIÓN EMPLEADAS PARA SU VALIDEZ EN CONTEXTOS CLÍNICOS Y EDUCATIVOS

    Directory of Open Access Journals (Sweden)

    Trinidad García Fernández

    2014-09-01

    Full Text Available Las funciones ejecutivas implican un amplio rango de habilidades relacionadas con la regulación del pensamiento, el comportamiento y el propio estado emocional. Teniendo en cuenta la variedad de componentes que forman este constructo, no es sorprendente que su evaluación plantee ciertas dificultades. En este sentido, pese a que numerosos estudios han mostrado la importancia de dichos componentes como determinantes del desempeño académico y social, así como su utilidad en el diagnóstico y la intervención en diversas problemáticas durante la infancia y la adolescencia, aspectos como la validez ecológica de las medidas tradicionalmente empleadas para su evaluación, imponen hoy ciertas limitaciones a la validez y generalización de los resultados obtenidos en este ámbito. El objetivo de este trabajo es analizar las características de las medidas de evaluación más ampliamente empleadas y su grado de aplicabilidad en contextos clínicos y educativos, de lo cual se desprenden una serie de implicaciones prácticas.

  13. LA LIBRE COMPETENCIA ECONÓMICA EN EL DERECHO COLOMBIANO: UNA REVISIÓN DESDE LA ECONOMÍA SOCIAL DE MERCADO Y SUS IMPLICACIONES NORMATIVAS

    Directory of Open Access Journals (Sweden)

    Andrea Alarcón Peña

    2016-01-01

    Full Text Available El artículo consigna un ejercicio de revisión jurídica sobre las características del modelo de economía social de mercado, reconocido como modelo económico por la Corte Constitucional, dentro del subsistema constitucional económico colombiano. l a primera parte del escrito de - talla las particularidades e implicaciones de este modelo y las consecuencias que su estructura supone para la libre competencia económica, habida consideración del marco normativo de protección. Posteriormente, se describen las generalidades de los sistemas de protección del derecho a la competencia anglosajón y europeo tomando como referencia las normas más importantes en cada uno de ellos, con el fin de comprender el impacto que dichos sistemas tuvieron en el modelo colombiano. Por último se presentan las propiedades más relevantes del régimen de protección de competencia en Colombia a partir de un bosquejo general del marco normativo existente.

  14. Canine adenovirus type 1 in a fennec fox (Vulpes zerda).

    Science.gov (United States)

    Choi, Jeong-Won; Lee, Hyun-Kyoung; Kim, Seong-Hee; Kim, Yeon-Hee; Lee, Kyoung-Ki; Lee, Myoung-Heon; Oem, Jae-Ku

    2014-12-01

    A 10-mo-old female fennec fox (Vulpes zerda) with drooling suddenly died and was examined postmortem. Histologic examination of different tissue samples was performed. Vacuolar degeneration and diffuse fatty change were observed in the liver. Several diagnostic methods were used to screen for canine parvovirus, canine distemper virus, canine influenza virus, canine coronavirus, canine parainfluenza virus, and canine adenovirus (CAdV). Only CAdV type 1 (CAdV-1) was detected in several organs (liver, lung, brain, kidney, spleen, and heart), and other viruses were not found. CAdV-1 was confirmed by virus isolation and nucleotide sequencing.

  15. Cryo-EM structure of human adenovirus D26 reveals the conservation of structural organization among human adenoviruses.

    Science.gov (United States)

    Yu, Xiaodi; Veesler, David; Campbell, Melody G; Barry, Mary E; Asturias, Francisco J; Barry, Michael A; Reddy, Vijay S

    2017-05-01

    Human adenoviruses (HAdVs) cause acute respiratory, ocular, and gastroenteric diseases and are also frequently used as gene and vaccine delivery vectors. Unlike the archetype human adenovirus C5 (HAdV-C5), human adenovirus D26 (HAdV-D26) belongs to species-D HAdVs, which target different cellular receptors, and is differentially recognized by immune surveillance mechanisms. HAdV-D26 is being championed as a lower seroprevalent vaccine and oncolytic vector in preclinical and human clinical studies. To understand the molecular basis for their distinct biological properties and independently validate the structures of minor proteins, we determined the first structure of species-D HAdV at 3.7 Å resolution by cryo-electron microscopy. All the hexon hypervariable regions (HVRs), including HVR1, have been identified and exhibit a distinct organization compared to those of HAdV-C5. Despite the differences in the arrangement of helices in the coiled-coil structures, protein IX molecules form a continuous hexagonal network on the capsid exterior. In addition to the structurally conserved region (3 to 300) of IIIa, we identified an extra helical domain comprising residues 314 to 390 that further stabilizes the vertex region. Multiple (two to three) copies of the cleaved amino-terminal fragment of protein VI (pVIn) are observed in each hexon cavity, suggesting that there could be ≥480 copies of VI present in HAdV-D26. In addition, a localized asymmetric reconstruction of the vertex region provides new details of the three-pronged "claw hold" of the trimeric fiber and its interactions with the penton base. These observations resolve the previous conflicting assignments of the minor proteins and suggest the likely conservation of their organization across different HAdVs.

  16. A novel adenovirus of Western lowland gorillas (Gorilla gorilla gorilla

    Directory of Open Access Journals (Sweden)

    Ludwig Carsten

    2010-11-01

    Full Text Available Abstract Adenoviruses (AdV broadly infect vertebrate hosts including a variety of primates. We identified a novel AdV in the feces of captive gorillas by isolation in cell culture, electron microscopy and PCR. From the supernatants of infected cultures we amplified DNA polymerase (DPOL, preterminal protein (pTP and hexon gene sequences with generic pan primate AdV PCR assays. The sequences in-between were amplified by long-distance PCRs of 2 - 10 kb length, resulting in a final sequence of 15.6 kb. Phylogenetic analysis placed the novel gorilla AdV into a cluster of primate AdVs belonging to the species Human adenovirus B (HAdV-B. Depending on the analyzed gene, its position within the cluster was variable. To further elucidate its origin, feces samples of wild gorillas were analyzed. AdV hexon sequences were detected which are indicative for three distinct and novel gorilla HAdV-B viruses, among them a virus nearly identical to the novel AdV isolated from captive gorillas. This shows that the discovered virus is a member of a group of HAdV-B viruses that naturally infect gorillas. The mixed phylogenetic clusters of gorilla, chimpanzee, bonobo and human AdVs within the HAdV-B species indicate that host switches may have been a component of the evolution of human and non-human primate HAdV-B viruses.

  17. Single-cycle adenovirus vectors in the current vaccine landscape.

    Science.gov (United States)

    Barry, Michael

    2018-02-01

    Traditional inactivated and protein vaccines generate strong antibodies, but struggle to generate T cell responses. Attenuated pathogen vaccines generate both, but risk causing the disease they aim to prevent. Newer gene-based vaccines drive both responses and avoid the risk of infection. While these replication-defective (RD) vaccines work well in small animals, they can be weak in humans because they do not replicate antigen genes like more potent replication-competent (RC) vaccines. RC vaccines generate substantially stronger immune responses, but also risk causing their own infections. To circumvent these problems, we developed single-cycle adenovirus (SC-Ad) vectors that amplify vaccine genes, but that avoid the risk of infection. This review will discuss these vectors and their prospects for use as vaccines. Areas covered: This review provides a background of different types of vaccines. The benefits of gene-based vaccines and their ability to replicate antigen genes are described. Adenovirus vectors are discussed and compared to other vaccine types. Replication-defective, single-cycle, and replication-competent Ad vaccines are compared. Expert commentary: The potential utility of these vaccines are discussed when used against infectious diseases and as cancer vaccines. We propose a move away from replication-defective vaccines towards more robust replication-competent or single-cycle vaccines.

  18. Mucosal vaccination by adenoviruses displaying reovirus sigma 1

    Energy Technology Data Exchange (ETDEWEB)

    Weaver, Eric A. [Department of Internal Medicine, Division of Infectious Diseases, Translational Immunovirology and Biodefense Program, Mayo Clinic, Rochester, MN 55902 (United States); Camacho, Zenaido T. [Department of Cell Biology, Department of Natural Sciences, Western New Mexico University, Silver City, NM 88062 (United States); Hillestad, Matthew L. [Nephrology Training Program, Mayo Clinic, Rochester, MN 55902 (United States); Crosby, Catherine M.; Turner, Mallory A.; Guenzel, Adam J.; Fadel, Hind J. [Virology and Gene Therapy Graduate Program, Mayo Clinic, Rochester, MN 55902 (United States); Mercier, George T. [Department of Physics, University of Houston, Houston, TX 77004 (United States); Barry, Michael A., E-mail: mab@mayo.edu [Department of Internal Medicine, Division of Infectious Diseases, Translational Immunovirology and Biodefense Program, Mayo Clinic, Rochester, MN 55902 (United States); Department of Immunology and Department of Molecular Medicine, Mayo Clinic, Rochester, MN 55902 (United States)

    2015-08-15

    We developed adenovirus serotype 5 (Ad5) vectors displaying the sigma 1 protein from reovirus as mucosal vaccines. Ad5-sigma retargets to JAM-1 and sialic acid, but has 40-fold reduced gene delivery when compared to Ad5. While weaker at transduction, Ad5-sigma generates stronger T cell responses than Ad5 when used for mucosal immunization. In this work, new Ad5-fiber-sigma vectors were generated by varying the number of fiber β-spiral shaft repeats (R) between the fiber tail and sigma. Increasing chimera length led to decreasing insertion of these proteinsAd5 virions. Ad-R3 and R14 vectors effectively targeted JAM-1 in vitro while R20 did not. When wereused to immunize mice by the intranasal route, Ad5-R3-sigma produced higher serum and vaginal antibody responses than Ad5. These data suggest optimized Ad-sigma vectors may be useful vectors for mucosal vaccination. - Highlights: • Constructed adenoviruses (Ads) displaying different reovirus sigma 1 fusion proteins. • Progressively longer chimeras were more poorly encapsidated onto Ad virions. • Ad5-R3-sigma mediated better systemic and mucosal immune responses than Ad5.

  19. Mucosal vaccination by adenoviruses displaying reovirus sigma 1

    International Nuclear Information System (INIS)

    Weaver, Eric A.; Camacho, Zenaido T.; Hillestad, Matthew L.; Crosby, Catherine M.; Turner, Mallory A.; Guenzel, Adam J.; Fadel, Hind J.; Mercier, George T.; Barry, Michael A.

    2015-01-01

    We developed adenovirus serotype 5 (Ad5) vectors displaying the sigma 1 protein from reovirus as mucosal vaccines. Ad5-sigma retargets to JAM-1 and sialic acid, but has 40-fold reduced gene delivery when compared to Ad5. While weaker at transduction, Ad5-sigma generates stronger T cell responses than Ad5 when used for mucosal immunization. In this work, new Ad5-fiber-sigma vectors were generated by varying the number of fiber β-spiral shaft repeats (R) between the fiber tail and sigma. Increasing chimera length led to decreasing insertion of these proteinsAd5 virions. Ad-R3 and R14 vectors effectively targeted JAM-1 in vitro while R20 did not. When wereused to immunize mice by the intranasal route, Ad5-R3-sigma produced higher serum and vaginal antibody responses than Ad5. These data suggest optimized Ad-sigma vectors may be useful vectors for mucosal vaccination. - Highlights: • Constructed adenoviruses (Ads) displaying different reovirus sigma 1 fusion proteins. • Progressively longer chimeras were more poorly encapsidated onto Ad virions. • Ad5-R3-sigma mediated better systemic and mucosal immune responses than Ad5

  20. Recombinant adenovirus-mediated gene transfer suppresses experimental arthritis

    Directory of Open Access Journals (Sweden)

    E. Quattrocchi

    2011-09-01

    Full Text Available Collagen Induced Arthritis (CIA is a widely studied animal model to develop and test novel therapeutic approaches for treating Rheumatoid Arthritis (RA in humans. Soluble Cytotoxic T-Lymphocyte Antigen 4 (CTLA4-Ig, which binds B7 molecule on antigen presenting cells and blocks CD28 mediated T-lymphocyte activation, has been shown to ameliorate experimental autoimmune diseases such as lupus, diabetes and CIA. Objective of our research was to investigate in vivo the effectiveness of blocking the B7/CD28 T-lymphocyte co-stimulatory pathway, utilizing a gene transfer technology, as a therapeutic strategy against CIA. Replication-deficient adenoviruses encoding a chimeric CTLA4-Ig fusion protein, or β-galactosidase as control, have been injected intravenously once at arthritis onset. Disease activity has been monitored by the assessment of clinical score, paw thickness and type II collagen (CII specific cellular and humoral immune responses for 21 days. The adenovirally delivered CTLA4-Ig fusion protein at a dose of 2×108 pfu suppressed established CIA, whereas the control β-galactosidase did not significantly affect the disease course. CII-specific lymphocyte proliferation, IFNg production and anti-CII antibodies were significantly reduced by CTLA4-Ig treatment. Our results demonstrate that blockade of the B7/CD28 co-stimulatory pathway by adenovirus-mediated CTLA4-Ig gene transfer is effective in treating established CIA suggesting its potential in treating RA.

  1. Non-Replicating Adenovirus-Vectored Anthrax Vaccine

    International Nuclear Information System (INIS)

    Van Kampen, K. R.; Zhang, J.; Jex, E.; Tang, D. C.

    2007-01-01

    As bioterrorism is emerging as a national threat, it is urgent to develop a new generation of anthrax vaccines that can be rapidly produced and mass administered in an emergency setting. We have demonstrated that protective immunity against anthrax spores could be elicited in mice by intranasal administration of a non-replicating human adenovirus serotype 5 (Ad5)-derived vector encoding Bacillus anthracis protective antigen (PA) in a single-dose regimen. The potency of an Ad5 vector encoding PA was remarkably enhanced by codon optimization of the PA gene to match the tRNA pool found in human cells. This nasal vaccine can be mass-administered by non-medical personnel during a bioterrorist attack. In addition, replication-competent adenovirus (RCA)-free Ad5-vectored anthrax vaccines can be mass produced in PER.C6 cells in serum-free wave bioreactors and purified by column chromatography to meet a surge in demand. The non-replicating nature of this new generation of anthrax vaccine ensures an excellent safety profile for vaccines and the environment.(author)

  2. Adenovirus chromatin structure at different stages of infection

    Energy Technology Data Exchange (ETDEWEB)

    Daniell, E.; Groff, D.E.; Fedor, M.J.

    1981-12-01

    The authors investigated the structure of adenovirus deoxyribonecleic acid (DNA)-protein complexes in nuclei of infected cells by using micrococal nuclease. Parental (infecting) DNA was digested into multimers which had a unit fragment size that was indistinguishable from the size of the nucleosomal repeat of cellular chromatin. This pattern was maintained in parental DNA throughout infection. Similar repeating units were detected in hamster cells that were nonpermissive for human adenovirus and in cells pretreated with n-butyrate. Late in infection, the pattern of digestion of viral DNA was determined by two different experimental approaches. Nuclear DNA was electrophoresed, blotted, and hybridized with labeled viral sequences; in this procedure all virus-specific DNA was detected. This technique revealed a diffuse protected band of viral DNA that was smaller than 160 base pairs, but no discrete multimers. All regions of the genome were represented in the protected DNA. To examine the nuclease protection of newly replicated viral DNA, infected cells were labeled with (/sup 3/)thymidine after blocking of cellular DNA synthesis but not viral DNA synthesis. With this procedure they identified a repeating unit which was distinctly different from the cellular nucleosomal repeat. The authors found broad bands with midpoints at 200, 400, and 600 base pairs, as well as the limit digest material revealed by blotting. High-resolution acrylamide gel electrophoresis revealed that the viral species comprised a series of closely spaced bands ranging in size from less than 30 to 250 base pairs.

  3. Adenovirus gene transfer to amelogenesis imperfecta ameloblast-like cells.

    Directory of Open Access Journals (Sweden)

    Anton V Borovjagin

    Full Text Available To explore gene therapy strategies for amelogenesis imperfecta (AI, a human ameloblast-like cell population was established from third molars of an AI-affected patient. These cells were characterized by expression of cytokeratin 14, major enamel proteins and alkaline phosphatase staining. Suboptimal transduction of the ameloblast-like cells by an adenovirus type 5 (Ad5 vector was consistent with lower levels of the coxsackie-and-adenovirus receptor (CAR on those cells relative to CAR-positive A549 cells. To overcome CAR -deficiency, we evaluated capsid-modified Ad5 vectors with various genetic capsid modifications including "pK7" and/or "RGD" motif-containing short peptides incorporated in the capsid protein fiber as well as fiber chimera with the Ad serotype 3 (Ad3 fiber "knob" domain. All fiber modifications provided an augmented transduction of AI-ameloblasts, revealed following vector dose normalization in A549 cells with a superior effect (up to 404-fold of pK7/RGD double modification. This robust infectivity enhancement occurred through vector binding to both α(vβ3/α(vβ5 integrins and heparan sulfate proteoglycans (HSPGs highly expressed by AI-ameloblasts as revealed by gene transfer blocking experiments. This work thus not only pioneers establishment of human AI ameloblast-like cell population as a model for in vitro studies but also reveals an optimal infectivity-enhancement strategy for a potential Ad5 vector-mediated gene therapy for AI.

  4. Phylogenetic and pathogenic characterization of novel adenoviruses from long-tailed ducks (Clangula hyemalis)

    Science.gov (United States)

    Counihan, Katrina; Skerratt, Lee; Franson, J. Christian; Hollmen, Tuula E.

    2015-01-01

    Novel adenoviruses were isolated from a long-tailed duck (Clangula hyemalis) mortality event near Prudhoe Bay, Alaska in 2000. The long-tailed duck adenovirus genome was approximately 27 kb. A 907 bp hexon gene segment was used to design primers specific for the long-tailed duck adenovirus. Nineteen isolates were phylogenetically characterized based on portions of their hexon gene and 12 were most closely related to Goose adenovirus A. The remaining 7 shared no hexon sequences with any known adenoviruses. Experimental infections of mallards with a long-tailed duck reference adenovirus caused mild lymphoid infiltration of the intestine and paint brush hemorrhages of the mucosa and dilation of the intestine. This study shows novel adenoviruses from long-tailed ducks are diverse and provides further evidence that they should be considered in cases of morbidity and mortality in sea ducks. Conserved and specific primers have been developed that will help screen sea ducks for adenoviral infections.

  5. Immunizing Patients With Metastatic Melanoma Using Recombinant Adenoviruses Encoding MART-1 or gp100 Melanoma Antigens

    Science.gov (United States)

    Rosenberg, Steven A.; Zhai, Yifan; Yang, James C.; Schwartzentruber, Douglas J.; Hwu, Patrick; Marincola, Francesco M.; Topalian, Suzanne L.; Restifo, Nicholas P.; Seipp, Claudia A.; Einhorn, Jan H.; Roberts, Bruce; White, Donald E.

    2008-01-01

    Background: The characterization of the genes encoding melanoma-associated antigens MART-1 or gp100, recognized by T cells, has opened new possibilities for the development of immunization strategies for patients with metastatic melanoma. With the use of recombinant adenoviruses expressing either MART-1 or gp100 to immunize patients with metastatic melanoma, we evaluated the safety, immunologic, and potential therapeutic aspects of these immunizations. Methods: In phase I studies, 54 patients received escalating doses (between 107 and 1011 plaque-forming units) of recombinant adenovirus encoding either MART-1 or gp100 melanoma antigen administered either alone or followed by the administration of interleukin 2 (IL-2). The immunologic impact of these immunizations on the development of cellular and antibody reactivity was assayed. Results: Recombinant adenoviruses expressing MART-1 or gp100 were safely administered. One of 16 patients with metastatic melanoma receiving the recombinant adenovirus MART-1 alone experienced a complete response. Other patients achieved objective responses, but they had received IL-2 along with an adenovirus, and their responses could be attributed to the cytokine. Immunologic assays showed no consistent immunization to the MART-1 or gp100 transgenes expressed by the recombinant adenoviruses. High levels of neutralizing antibody were found in the pretreatment sera of the patients. Conclusions: High doses of recombinant adenoviruses could be safely administered to cancer patients. High levels of neutralizing antibody present in patients' sera prior to treatment may have impaired the ability of these viruses to immunize patients against melanoma antigens. PMID:9862627

  6. IMPLICACIONES DEL USO DE BUSCADORES EN EL COMPORTAMIENTO DE COMPRA ONLINE

    Directory of Open Access Journals (Sweden)

    Ruiz Mafé, C.

    2009-01-01

    Full Text Available El objetivo del presente trabajo es analizar los factores que influyen en el uso de buscadores y su incidencia en el comportamiento de compra en Internet. El análisis de resultados obtenidos a partir de una muestra representativa de 485 compradores por Internet españoles, pone de manifiesto que los consumidores que consideran la comodidad, reducciones de precio y amplitud de surtido como motivaciones relevantes para realizar compras online presentan una mayor predisposición al uso de buscadores en la realización de sus compras virtuales. La experiencia como internauta y como comprador en Internet son factores que influyen negativamente en el uso de buscadores para la realización de compras virtuales. En cuanto a las implicaciones, los resultados no muestran una influencia significativa del uso de buscadores en el volumen de gasto en Internet.

  7. Implicaciones jurídicas de los alimentos transgénicos en Colombia

    Directory of Open Access Journals (Sweden)

    Mario Ernesto García Martínez

    2003-01-01

    Full Text Available Las implicaciones juridicas de los alimentos transgénicos en Colombia demuestra que es necesario que se establezca un marco legal de Bioseguridad sólido. Es imperativo que la autoridad medioambiental, entendiendo por éstá no sólo el Ministerio del Medio Ambiente sino también, el Ministerio de Salud, el Ministerio de Agricultura, el lCA y ellNVlMA, desarrollen una política integral de Bioseguridad y Biotecnología, y como consecuencia de ello presenten cuanto antes un proyecto de Ley Marco que se encargue de regular la Biotecnología en Colombia

  8. Adenovirus urethritis and concurrent conjunctivitis: a case series and review of the literature.

    Science.gov (United States)

    Liddle, Olivia Louise; Samuel, Mannampallil Itty; Sudhanva, Malur; Ellis, Joanna; Taylor, Chris

    2015-03-01

    We present eight cases and review the literature of concurrent urethritis and conjunctivitis where adenovirus was identified as the causative pathogen. The focus of this review concerns the identification of specific sexual practices, symptoms, signs and any serotypes that seem more commonly associated with such adenovirus infections. We discuss the seasonality of adenovirus infection and provide practical advice for clinicians to give to the patient. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  9. Significados e implicaciones de la alfabetización tecnológica en el desarrollo de niños, niñas y adolescentes

    Directory of Open Access Journals (Sweden)

    María López

    2014-08-01

    Full Text Available Se analizan los significados y algunas implicaciones de la alfabetización tecnológica en el desarrollo de niños, niñas y adolescentes, con base en referentes teóricos que permiten contextualizar el objeto y actores de estudio en una perspectiva humana integral, la cual combina lo cognitivo con lo psico-social. La metodología utilizada consistió en el diseño de un programa de alfabetización tecnológica, dirigido a una población de 60 niños, niñas y adolescentes pertenecientes al Sector El Marite, Parroquia Venancio Pulgar del Municipio Maracaibo, en virtud de que se asumió que dichos actores se encuentran en situación de exclusión social, con escasas posibilidades económicas para el acceder a la infraestructura tecnológica. Se aplicaron tres pruebas específicas: la subescala de Claves A-B, la subescala de Figuras Incompletas y la subescala Laberintos, pertenecientes a la prueba WISC-R de Weschler, que mide habilidades como: percepción visual y motriz, atención, coordinación y memoria. Adicionalmente, se aplicaron técnicas de dibujo proyectivo para develar la concepción que los niños, niñas y adolescentes tienen con relación a la tecnología. También se aplicó la escala Conners, contentiva de 59 items que miden 13 categorías del comportamiento. Los resultados revelan, además de la efectividad del programa en cuanto al desarrollo de competencias para la interacción tecnológica, un impacto significativo en el desarrollo intelectual y socio-emocional de la población atendida. Se concluye, que los programas de alfabetización tecnológica deben trascender la perspectiva instrumentalista para ubicarse en una dimensión más humana que entiende la tecnología como un elemento de desarrollo e inclusión social.

  10. LAS CONCEPCIONES DE LOS ALUMNOS SOBRE EL MEDIO URBANO Y SUS IMPLICACIONES DIDÁCTICAS. UN ESTUDIO EN BOGOTÁ

    Directory of Open Access Journals (Sweden)

    J. Francisco González Puentes

    2014-12-01

    Full Text Available Se presenta el marco teórico, la metodología y, sobre todo, los resultados de una tesis doctoral, realizada con una muestra de alumnos de secundaria de Bogotá, que tuvo como objeto estudiar las concepciones de dichos alumnos sobre el medio urbano, los obstáculos que éstas conllevan y sus implicaciones didácticas. El tratamiento de los datos se realiza con cuatro categorías básicas: problemas urbanos, espacio público, participación ciudadana y calidad de vida, que permiten estructurar el medio urbano como un “ámbito de conocimiento escolar”. De ahí se obtienen los resultados y las conclusiones, así como orientaciones para el diseño y desarrollo de propuestas de enseñanza sobre el medio urbano.  Palabras clave: Concepciones de los alumnos sobre el medio urbano, problemas urbanos, espacio público, participación ciudadana, calidad de vida. Abstract: This paper presents the theoretical framework, methodology, and, above all, the results of a doctoral thesis, carried out with a sample of high school students in Bogotá, which had as objective to study these students’ conceptions about urban environment, the obstacles they involve and their educational implications. The data processing is carried out with four basic categories, urban problems, public space, citizen participation and quality of life, which allow to structure urban environment as a “field of school-level knowledge”. Thus, results and conclusions are obtained as well as guidelines for the design and development of teaching proposals on urban environment. Key Words: Students’ conceptions on urban environment, urban problems, public space, citizens’ participation, quality of life. Résumé: On présente le cadre théorique, la méthodologie, et, surtout, les résultats d’une thèse de doctorat, menée auprès d’un échantillon d’élèves d’une école secondaire de Bogota, qui a eu pour objectif d’étudier les représentations de ces

  11. PCR Analysis of Egyptian Respiratory Adenovirus Isolates, Including Identification of Species, Serotypes, and Coinfections

    National Research Council Canada - National Science Library

    Metzgar, David; Osuna, Miguel; Yingst, Samuel; Rakha, Magda; Earhart, Kenneth; Elyan, Diaa; Esmat, Hala; Saad, Magdi D; Kajon, Adriana; Wu, Jianguo; Gray, Gregory C; Ryan, Margaret A; Russell, Kevin L

    2005-01-01

    Eighty-eight adenovirus (Ad) isolates and associated clinical data were collected from walk-in patients with influenza-like illness in Egypt during routine influenza surveillance from 1999 through 2002...

  12. Full genome analysis of a novel adenovirus from the South Polar skua (Catharacta maccormicki) in Antarctica.

    Science.gov (United States)

    Park, Yon Mi; Kim, Jeong-Hoon; Gu, Se Hun; Lee, Sook Young; Lee, Min-Goo; Kang, Yoon Kyoo; Kang, Sung-Ho; Kim, Hak Jun; Song, Jin-Won

    2012-01-05

    Adenoviruses have been identified in humans and a wide range of vertebrate animals, but not previously from the polar region. Here, we report the entire 26,340-bp genome of a novel adenovirus, detected by PCR, in tissues of six of nine South Polar skuas (Catharacta maccormicki), collected in Lake King Sejong, King George Island, Antarctica, from 2007 to 2009. The DNA polymerase, penton base, hexon and fiber genes of the South Polar skua adenovirus (SPSAdV) exhibited 68.3%, 75.4%, 74.9% and 48.0% nucleotide sequence similarity with their counterparts in turkey hemorrhagic enteritis virus. Phylogenetic analysis based on the entire genome revealed that SPSAdV belonged to the genus Siadenovirus, family Adenoviridae. This is the first evidence of a novel adenovirus, SPSAdV, from a large polar seabird (family Stercorariidae) in Antarctica. Copyright © 2011 Elsevier Inc. All rights reserved.

  13. New adenoviruses from new primate hosts - growing diversity reveals taxonomic weak points

    Czech Academy of Sciences Publication Activity Database

    Dadáková, E.; Chrudimský, Tomáš; Brožová, K.; Modrý, David; Celer, V.; Hrazdilová, K.

    2017-01-01

    Roč. 107, February (2017), s. 305-307 ISSN 1055-7903 Institutional support: RVO:60077344 Keywords : adenovirus * primate * phylogeny * taxonomy Subject RIV: EE - Microbiology, Virology OBOR OECD: Microbiology Impact factor: 4.419, year: 2016

  14. Detection of enteric Adenoviruses in South-African waters using gene probes

    CSIR Research Space (South Africa)

    Genthe, Bettina

    1995-01-01

    Full Text Available Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultra filtration and analysed...

  15. Partial characterization of new adenoviruses found in lizards.

    Science.gov (United States)

    Ball, Inna; Behncke, Helge; Schmidt, Volker; Geflügel, F T A; Papp, Tibor; Stöhr, Anke C; Marschang, Rachel E

    2014-06-01

    In the years 2011-2012, a consensus nested polymerase chain reaction was used for the detection of adenovirus (AdV) infection in reptiles. During this screening, three new AdVs were detected. One of these viruses was detected in three lizards from a group of green striped tree dragons (Japalura splendida). Another was detected in a green anole (Anolis carolinensis). A third virus was detected in a Jackson's chameleon (Chamaeleo jacksonii). Analysis of a portion of the DNA-dependent DNA polymerase genes of each of these viruses revealed that they all were different from one another and from all previously described reptilian AdVs. Phylogenetic analysis of the partial DNA polymerase gene sequence showed that all newly detected viruses clustered within the genus Atadenovirus. This is the first description of AdVs in these lizard species.

  16. Development of an immunotherapeutic adenovirus targeting hormone-independent prostate cancer

    Directory of Open Access Journals (Sweden)

    Kim JS

    2013-11-01

    Full Text Available Jae Sik Kim,1 Sang Don Lee,2 Sang Jin Lee,3 Moon Kee Chung21Department of Urology, The Catholic University of Korea Incheon St Mary's Hospital, Incheon, 2Pusan National University Yangsan Hospital and Research Institute for Convergence of Biomedical Science and Technology, Yangsan, 3Genitourinary Cancer Branch, National Cancer Center, Goyang, KoreaBackground: To develop a targeting therapy for hormone-independent prostate cancer, we constructed and characterized conditionally replicating oncolytic adenovirus (Ad equipped with mRFP(monomeric red fluorescence protein/ttk (modified herpes simplex virus thymidine kinase This construct was then further modified to express both mRFP/ttk and a soluble form of cytokine FLT3L (fms-related tyrosine kinase 3 ligand simultaneously.Methods: To construct the recombinant oncolytic adenovirus, E1a and E4 genes, which are necessary for adenovirus replication, were controlled by the prostate-specific enhancer sequence (PSES targeting prostate cancer cells expressing prostate-specific antigen (PSA and prostate-specific membrane antigen (PSMA. Simultaneously, it expressed the mRFP/ttk fusion protein in order to be able to elicit the cytotoxic effect.Results: The Ad5/35PSES.mRFP/ttk chimeric recombinant adenovirus was generated successfully. When replication of Ad5/35PSES.mRFP/ttk was evaluated in prostate cancer cell lines under fluorescence microscopy, red fluorescence intensity increased more in LNCaP cells, suggesting that the mRFP/ttk fusion protein was folded functionally. In addition, the replication assay including wild-type adenovirus as a positive control showed that PSES-positive cells (LNCaP and CWR22rv permitted virus replication but not PSES-negative cells (DU145 and PC3. Next, we evaluated the killing activity of this recombinant adenovirus. The Ad5/35PSES.mRFP/ttk killed LNCaP and CWR22rv more effectively. Unlike PSES-positive cells, DU145 and PC3 were resistant to killing by this recombinant

  17. Bipartite structure and functional independence of adenovirus type 5 packaging elements.

    OpenAIRE

    Schmid, S I; Hearing, P

    1997-01-01

    Selectivity and polarity of adenovirus type 5 DNA packaging are believed to be directed by an interaction of putative packaging factors with the cis-acting adenovirus packaging domain located within the genomic left end (nucleotides 194 to 380). In previous studies, this packaging domain was mutationally dissected into at least seven functional elements called A repeats. These elements, albeit redundant in function, exhibit differences in the ability to support viral packaging, with elements ...

  18. cis and trans requirements for the selective packaging of adenovirus type 5 DNA.

    OpenAIRE

    Gräble, M; Hearing, P

    1992-01-01

    Polar packaging of adenovirus DNA into virions is dependent on the presence of cis-acting sequences at the left end of the viral genome. Our previous analyses demonstrated that the adenovirus type 5 (Ad5) packaging domain (nucleotides 194 to 358) is composed of at least five elements that are functionally redundant. A repeated sequence, termed the A repeat, was associated with packaging function. Here we report a more detailed analysis of the requirements for the selective packaging of Ad5 DN...

  19. Formation of a Multiple Protein Complex on the Adenovirus Packaging Sequence by the IVa2 Protein▿

    OpenAIRE

    Tyler, Ryan E.; Ewing, Sean G.; Imperiale, Michael J.

    2007-01-01

    During adenovirus virion assembly, the packaging sequence mediates the encapsidation of the viral genome. This sequence is composed of seven functional units, termed A repeats. Recent evidence suggests that the adenovirus IVa2 protein binds the packaging sequence and is involved in packaging of the genome. Study of the IVa2-packaging sequence interaction has been hindered by difficulty in purifying the protein produced in virus-infected cells or by recombinant techniques. We report the first ...

  20. Detection of a putative novel adenovirus by PCR amplification, sequencing and phylogenetic characterisation of two gene fragments from formalin-fixed paraffin-embedded tissues of a cat diagnosed with disseminated adenovirus disease.

    Science.gov (United States)

    Lakatos, Béla; Hornyák, Ákos; Demeter, Zoltán; Forgách, Petra; Kennedy, Frances; Rusvai, Miklós

    2017-12-01

    Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in formalin-fixed paraffin-embedded tissue samples of a cat that had suffered from disseminated adenovirus infection. The identity of the amplified products from the hexon and DNA-dependent DNA polymerase genes was confirmed by DNA sequencing. The sequences were clearly distinguishable from corresponding hexon and polymerase sequences of other mastadenoviruses, including human adenoviruses. These results suggest the possible existence of a distinct feline adenovirus.

  1. Getting genetic access to natural adenovirus genomes to explore vector diversity.

    Science.gov (United States)

    Zhang, Wenli; Ehrhardt, Anja

    2017-10-01

    Recombinant vectors based on the human adenovirus type 5 (HAdV5) have been developed and extensively used in preclinical and clinical studies for over 30 years. However, certain restrictions of HAdV5-based vectors have limited their clinical applications because they are rather inefficient in specifically transducing cells of therapeutic interest that lack the coxsackievirus and adenovirus receptor (CAR). Moreover, enhanced vector-associated toxicity and widespread preexisting immunity have been shown to significantly hamper the effectiveness of HAdV-5-mediated gene transfer. However, evolution of adenoviruses in the natural host is driving the generation of novel types with altered virulence, enhanced transmission, and altered tissue tropism. As a consequence, an increasing number of alternative adenovirus types were identified, which may represent a valuable resource for the development of novel vector types. Thus, researchers are focusing on the other naturally occurring adenovirus types, which are structurally similar but functionally different from HAdV5. To this end, several strategies have been devised for getting genetic access to adenovirus genomes, resulting in a new panel of adenoviral vectors. Importantly, these vectors were shown to have a host range different from HAdV5 and to escape the anti-HAdV5 immune response, thus underlining the great potential of this approach. In summary, this review provides a state-of-the-art overview of one essential step in adenoviral vector development.

  2. Replication-competent human adenovirus 11p vectors can propagate in Vero cells

    International Nuclear Information System (INIS)

    Gokumakulapalle, Madhuri; Mei, Ya-Fang

    2016-01-01

    The use of continuous cell lines derived from the African green monkey kidney (AGMK) has led to major advances in virus vaccine development. However, to date, these cells have not been used to facilitate the creation of human adenoviruses because most human adenoviruses undergo abortive infections in them. Here, we report the susceptibility of AGMK-derived cells to adenovirus 11p (Ad11p) infection. First, we showed that CD46 molecules, which act as receptors for Ad11p, are expressed in AGMK cells. We then monitored Ad11p replication by measuring GFP expression as an indicator of viral transcription. We found that AGMK-derived cells were as capable as carcinoma cells at propagating full-length replication-competent Ad11p (RCAd11p) DNA. Of the AGMK cell lines tested, Vero cells had the greatest capacity for adenovirus production. Thus, AGMK cells can be used to evaluate RCAd11p-mediated gene delivery, and Vero cells can be used for the production of RCAd11pGFP vectors at relatively high yields. - Highlights: • Africa green monkey cell lines were monitored for human adenovirus 11p GFP vector infection. • Human CD46 molecules were detectable in these monkey cell lines. • Adenovirus 11p GFP vector can be propagated in Vero cells increases the safety of Ad11p-based vectors for clinical trials. • To use Vero cells for preparation of Ad11p vector avoids the potential inclusion of oncogenes from tumor cells.

  3. A molecular epidemiology survey of respiratory adenoviruses circulating in children residing in Southern Palestine.

    Directory of Open Access Journals (Sweden)

    Lina Qurei

    Full Text Available A molecular epidemiology survey was performed in order to establish and document the respiratory adenovirus pathogen profiles among children in Southern Palestine. Three hundred and thirty-eight hospitalized pediatric cases with adenovirus-associated respiratory tract infections were analyzed. Forty four cases out of the 338 were evaluated in more detail for the adenoviruses types present. All of the children resided in Southern Palestine, that is, in city, village and refugee camp environments within the districts of Hebron and Bethlehem. Human adenoviruses circulated throughout 2005-2010, with major outbreaks occurring in the spring months. A larger percent of the children diagnosed with adenoviral infections were male infants. DNA sequence analysis of the hexon genes from 44 samples revealed that several distinct adenovirus types circulated in the region; these were HAdV-C1, HAdV-C2, HAdV-B3 and HAdV-C5. However, not all of these types were detected within each year. This is the first study ever conducted in Palestine of the genetic epidemiology of respiratory adenovirus infections.

  4. Tip60 degradation by adenovirus relieves transcriptional repression of viral transcriptional activator EIA.

    Science.gov (United States)

    Gupta, A; Jha, S; Engel, D A; Ornelles, D A; Dutta, A

    2013-10-17

    Adenoviruses are linear double-stranded DNA viruses that infect human and rodent cell lines, occasionally transform them and cause tumors in animal models. The host cell challenges the virus in multifaceted ways to restrain viral gene expression and DNA replication, and sometimes even eliminates the infected cells by programmed cell death. To combat these challenges, adenoviruses abrogate the cellular DNA damage response pathway. Tip60 is a lysine acetyltransferase that acetylates histones and other proteins to regulate gene expression, DNA damage response, apoptosis and cell cycle regulation. Tip60 is a bona fide tumor suppressor as mice that are haploid for Tip60 are predisposed to tumors. We have discovered that Tip60 is degraded by adenovirus oncoproteins EIB55K and E4orf6 by a proteasome-mediated pathway. Tip60 binds to the immediate early adenovirus promoter and suppresses adenovirus EIA gene expression, which is a master regulator of adenovirus transcription, at least partly through retention of the virally encoded repressor pVII on this promoter. Thus, degradation of Tip60 by the adenoviral early proteins is important for efficient viral early gene transcription and for changes in expression of cellular genes.

  5. Replication-competent human adenovirus 11p vectors can propagate in Vero cells

    Energy Technology Data Exchange (ETDEWEB)

    Gokumakulapalle, Madhuri; Mei, Ya-Fang, E-mail: ya-fang.mei@umu.se

    2016-08-15

    The use of continuous cell lines derived from the African green monkey kidney (AGMK) has led to major advances in virus vaccine development. However, to date, these cells have not been used to facilitate the creation of human adenoviruses because most human adenoviruses undergo abortive infections in them. Here, we report the susceptibility of AGMK-derived cells to adenovirus 11p (Ad11p) infection. First, we showed that CD46 molecules, which act as receptors for Ad11p, are expressed in AGMK cells. We then monitored Ad11p replication by measuring GFP expression as an indicator of viral transcription. We found that AGMK-derived cells were as capable as carcinoma cells at propagating full-length replication-competent Ad11p (RCAd11p) DNA. Of the AGMK cell lines tested, Vero cells had the greatest capacity for adenovirus production. Thus, AGMK cells can be used to evaluate RCAd11p-mediated gene delivery, and Vero cells can be used for the production of RCAd11pGFP vectors at relatively high yields. - Highlights: • Africa green monkey cell lines were monitored for human adenovirus 11p GFP vector infection. • Human CD46 molecules were detectable in these monkey cell lines. • Adenovirus 11p GFP vector can be propagated in Vero cells increases the safety of Ad11p-based vectors for clinical trials. • To use Vero cells for preparation of Ad11p vector avoids the potential inclusion of oncogenes from tumor cells.

  6. Adenovirus Particles that Display the Plasmodium falciparum Circumsporozoite Protein NANP Repeat Induce Sporozoite-Neutralizing Antibodies in Mice

    OpenAIRE

    Palma, Christopher; Overstreet, Michael G.; Guedon, Jean-Marc; Hoiczyk, Egbert; Ward, Cameron; Karen, Kasey A.; Zavala, Fidel; Ketner, Gary

    2011-01-01

    Adenovirus particles can be engineered to display exogenous peptides on their surfaces by modification of viral capsid proteins, and particles that display pathogen-derived peptides can induce protective immunity. We constructed viable recombinant adenoviruses that display B-cell epitopes from the Plasmodium falciparum circumsporozoite protein (PfCSP) in the major adenovirus capsid protein, hexon. Recombinants induced high-titer antibodies against CSP when injected intraperitoneally into mice...

  7. La Legitimación de Capitales y sus implicaciones económicas. Una aproximación a la conceptualización de este delito

    Directory of Open Access Journals (Sweden)

    Yosman Valderrama

    2015-09-01

    Full Text Available La legitimación de capitales es un fenómeno criminal de dimensiones internacionales, cristaliza el esfuerzo de la delincuencia organizada por darle apariencia legal a un dinero obtenido por actividades ilícitas. Con el propósito de conocer este fenómeno, la presente investigación tuvo como objetivo estudiar la legitimación de capitales y sus implicaciones económicas, tomando en consideración los constructos teóricos establecidos por los autores Cano y Lugo (2010, Perotti (2009, y Del Cid (2007. Metodológicamente, el estudio es una revisión documental y como instrumento de recolección de datos se acudió al uso del resumen analítico y el análisis crítico. Entre las consideraciones finales, este quebrantamiento normativo presenta efectos adversos sobre la economía de una nación, que pueden variar de acuerdo a las características particulares de cada país, dentro de las consecuencias más resaltantes destacan: incide en la volatilidad de la economía, repercute en el establecimiento de las políticas económicas, así como en la capacidad productiva nacional, promueve la competencia desleal, genera insatisfacción entre los sectores productivos, disminuye la credibilidad de las instituciones, desmejora la libre oferta y demanda de los productos de consumo masivo. No obstante, puede representar ficticiamente soluciones a problemas económicos, como la generación de empleo, el aumento en la aplicación de nueva tecnología en la producción agrícola, crecimiento de la construcción de infraestructuras, y hace visible un desarrollo económico del país que la sufre.

  8. Mercado de trabajo de personas con discapacidad: teoría, política y aplicaciones

    OpenAIRE

    Cazallas Alcaide, Carlos

    2016-01-01

    En el contexto socioeconómico actual, la obtención y desempeño de un puesto de trabajo supone para cualquier individuo un hecho determinante, por las implicaciones que conlleva en cuanto a independencia económica, de desarrollo humano, de integración en la sociedad, de generación de un sentimiento de utilidad social y de mejora de la autoestima del individuo por la vía del reconocimiento social. Para las personas con discapacidad además va a suponer la potenciación de unas capacidades y habil...

  9. Up-regulation of integrin β3 in radioresistant pancreatic cancer impairs adenovirus-mediated gene therapy

    International Nuclear Information System (INIS)

    Egami, Takuya; Ohuchida, Kenoki; Yasui, Takaharu; Onimaru, Manabu; Toma, Hiroki; Sato, Norihiro; Tanaka, Masao; Mizumoto, Kazuhiro; Matsumoto, Kunio

    2009-01-01

    Adenovirus-mediated gene therapy is a promising approach for the treatment of pancreatic cancer. We previously reported that radiation enhanced adenovirus-mediated gene expression in pancreatic cancer, suggesting that adenoviral gene therapy might be more effective in radioresistant pancreatic cancer cells. In the present study, we compared the transduction efficiency of adenovirus-delivered genes in radiosensitive and radioresistant cells, and investigated the underlying mechanisms. We used an adenovirus expressing the hepatocyte growth factor antagonist, NK4 (Ad-NK4), as a representative gene therapy. We established two radioresistant human pancreatic cancer cell lines using fractionated irradiation. Radiosensitive and radioresistant pancreatic cancer cells were infected with Ad-NK4, and NK4 levels in the cells were measured. In order to investigate the mechanisms responsible for the differences in the transduction efficiency between these cells, we measured expression of the genes mediating adenovirus infection and endocytosis. The results revealed that NK4 levels in radioresistant cells were significantly lower (P<0.01) than those in radiosensitive cells, although there were no significant differences in adenovirus uptake between radiosensitive cells and radioresistant cells. Integrin β3 was up-regulated and the Coxsackie virus and adenovirus receptor was down-regulated in radioresistant cells, and inhibition of integrin β3 promoted adenovirus gene transfer. These results suggest that inhibition of integrin β3 in radioresistant pancreatic cancer cells could enhance adenovirus-mediated gene therapy. (author)

  10. Polimorfismos en el gen promotor de IL-10 en una muestra de pacientes colombianos con lepra

    Directory of Open Access Journals (Sweden)

    Nora Cardona-Castro

    2012-03-01

    Conclusiones. El haplotipo que encontramos asociado con lepra, -1082A-819C-592C/-1082A-819C-592C, se ha relacionado con baja producción de IL-10. Funcionalmente, esta baja producción de IL-10 puede tener consecuencias en la respuesta inmunitaria, además de implicaciones clínicas. Se han reportado diferentes haplotipos de IL-10 como marcadores de vulnerabilidad y resistencia de lepra en otras poblaciones, lo cual sugiere que las diferencias en la distribución de diversos polimorfismos del gen de IL-10 entre grupos étnicos, es un factor importante al determinar la asociación entre enfermedad y genes.   DOI: http://dx.doi.org/10.7705/biomedica.v32i1.386

  11. LA AFECTACIÓN PRESUPUESTARIA Y SUS IMPLICACIONES EN LA ORGANIZACIÓN ADMINISTRATIVA Y EN EL SISTEMA CONTABLE DE LAS ENTIDADES RELIGIOSAS Y LA ADMINISTRACIÓN PÚBLICA DESDE UNA PERSPECTIVA HISTÓRICA

    Directory of Open Access Journals (Sweden)

    Susana Villaluenga

    2016-07-01

    Full Text Available Si comparamos el sistema económico, administrativo y financiero de la Iglesia y de la Hacienda Pública castellana en la Edad Moderna observamos grandes similitudes. Nada de extrañar si consideramos que la Iglesia fiscalizaba a los fieles y la Monarquía a los ciudadanos. La red recaudatoria clerical llegaba hasta las parroquias como la Real a todos los rincones del Estado y confluía en centros de recaudación y de responsabilidad, cuya actividad controlaban los contadores eclesiásticos y los públicos, respectivamente. La contabilidad eclesiástica era administrativa y presupuestaria como la del Estado. En este contexto regía el principio de afectación, donde los gastos se financian con ingresos específicos a ellos afectados que deberán especificarse y permitir su seguimiento. Un principio contrario al principio de no afectación que rige actualmente en la Administración Pública.    En este trabajo demostraremos cómo la afectación de los gastos a los ingresos eclesiásticos es un principio relacionado con la destinación de los bienes presente en la doctrina de la Iglesia. Asimismo, probaremos que la aplicación de este principio tuvo implicaciones en la organización administrativa, en el sistema contable y en los dispositivos de control.

  12. Competencia cognitiva en penados primarios y reincidentes: implicaciones para la reeducación

    Directory of Open Access Journals (Sweden)

    Ramón Arce

    2014-01-01

    Full Text Available La probabilidad de reincidencia delictiva es aproximadamente tres veces mayor para los penados reincidentes que para los primarios. En esta línea, se sugiere una relación entre el grado de comportamiento antisocial y delictivo, y el nivel de desarrollo de la competencia cognitiva. Así, se prevé que los penados reincidentes presenten un menor desarrollo de la competencia cognitiva que los primarios. Para comprobar esta hipótesis, 104 penados, 54 primarios y 50 reincidentes, que no habían sido previamente objeto de un tratamiento penitenciario, fueron evaluados en atribución de la responsabilidad, autoconcepto, afrontamiento e inteligencia emocional. Los resultados mostraron que los penados reincidentes tenían menos desarrollado el autoconcepto y la inteligencia emocional que los primarios. Además, se observó una mayor tendencia en los reincidentes a afrontar las situaciones estresantes y de riesgo a través de estrategias no adaptativas (v.gr., centradas en las emociones, evitativas. Sin embargo, primarios y reincidentes se atribuyen por igual la responsabilidad. En suma, los penados reincidentes son menos competentes cognitivamente que los primarios. Se discuten las implicaciones de los resultados para la reeducación, resocialización y reinserción social de los penados.

  13. Implicaciones ambientales de las tecnologías de energía renovable

    Directory of Open Access Journals (Sweden)

    Jan Mahirt-Smith

    2011-12-01

    Full Text Available Las tecnologías de energía renovable como la eólica, la solar y la biomasa, hacen un uso del suelo más intenso que las de combustibles fósiles tradicionales y, geográficamente, sus implicaciones ambientales son más heterogéneas, por lo que presentan un gran desafío para las técnicas de evaluación de su ciclo de vida. Este trabajo presenta los resultados de una investigación bibliográfica alrededor de los siguientes temas: 1 cambios en el uso del suelo debido a la mayor producción de energía renovable; 2 impactos del uso de suelo; 3 variabilidad geográfica en el inventario de datos; y 4 efectos de la distribución de energía. Además, se revisa el grado de investigación que actualmente se aplica acerca de las tecnologías de energía renovable en campos como el eólico, el solar y la bioenergía y en la evaluación del ciclo de vida en general.

  14. IMPLICACIONES SANITARIAS DEL ESTABLECIMIENTOY EXPANSION EN ESPAÑA DEL MOSQUITO AEDES ALBOPICTUS

    Directory of Open Access Journals (Sweden)

    Rubén Bueno Marí

    2012-01-01

    Full Text Available La expansión de Aedes albopictus por el Levante español no ha cesado desde su primigenia detección en nuestro país en el año 2004, habiéndose constatado ya su presencia en las provincias de Girona, Barcelona, Tarragona, Castellón, Alicante y Murcia. La elevada sinantropía de la especie, unida al alto grado de antropofília y extensa capacidad vectorial para transmitir diversas arbovirosis, han propiciado el retorno al sur de Europa de ciclos de transmisión activa de enfermedades frecuentes en el pasado como el Dengue o la aparición de virosis tropicales inéditas hasta entonces como Chikungunya. El presente manuscrito analiza de forma pormenorizada las implicaciones para la salud pública de la previsible expansión de Ae. albopictus por gran parte del territorio peninsular, en el contexto climático y sociodemográfico actual. Adicionalmente, también se discuten diversas cuestiones relativas al control poblacional de la especie en ambientes urbanos y periurbanos, así como los datos preliminares existentes acerca de otros aedinos invasores de reciente hallazgo en el continente europeo.

  15. Patients with acute myocardial infarction and interfering factors when seeking emergency care: implications for health education Pacientes con infarto del miocárdio agudo y los factores que interfieren en la búsqueda de un servicio de emergencia: implicaciones para la educación en salud Pacientes com infarto agudo do miocárdio e os fatores que interferem na procura por serviço de emergência: implicações para a educação em saúde

    Directory of Open Access Journals (Sweden)

    Betina Franco

    2008-06-01

    Full Text Available With the objective of estimating the time elapsed between the beginning of the signs and symptoms of a heart attack until the arrival at the cardiology emergency service (Delta T and the factors that influence in this process, 112 patients were studied, with an infarction diagnosis with supraunleveling ST segment. The delta T was on average of 3h59±2h55min; 99(88% of those patients sought out an emergency service within 1h after the beginning of the event. Unmarried patients presented a delta T smaller in relation to the others (P=0,006, as well as those that recognized the symptoms as a heart event; thoracic pain with burning symptoms, were described by 25 (24% of patients, and the first attitude taken, in view those symptoms, was self-medication 37(33%. The recognition of the signs and symptoms of Acute Myocardium Infarction, is a decisive factor for the seeking of a specialized service.Con el objetivo de estimar el tiempo transcurrido entre el inicio de las señales y síntomas del infarto hasta la llegada al sector de emergencia cardiológica (delta T y los factores que influenciaron ese proceso, se estudiaron 112 pacientes, con diagnóstico de infarto con elevación del segmento ST. El delta T fue en promedio de 3h59min±2h55min, siendo que 99(88% de esos pacientes buscaron un servicio de emergencia una hora después el inicio del evento. Pacientes solteros presentaron un delta T menor en relación a los demás (P=0,006, así como aquellos que reconocieron los síntomas como un evento cardíaco; dolor torácico con ardor fue relatado por 25(24% pacientes, siendo que la primera actitud tomada ante esos síntomas fue la automedicación 37(33%. Se concluyó que el reconocimiento de las señales y de los síntomas de IMA por el paciente es un factor determinante en la búsqueda de atención especializada.Com o objetivo de estimar o tempo decorrido entre o início dos sinais e sintomas do infarto até a chegada ao setor de emergência cardiol

  16. Trabajadores con discapacidad: problemas, retos y principios de actuación en salud ocupacional

    Directory of Open Access Journals (Sweden)

    Carlos María Alcover de la Hera

    Full Text Available La participación y normalización de las personas con discapacidad en el mercado de trabajo depende tanto de factores externos a la organización, como el contexto legal, económico u ocupacional, como de factores relacionados con la gestión de las personas en las empresas, entre los que destacan la cultura organizacional, las políticas y prácticas de recursos humanos, los estilos de supervisión y dirección y las relaciones entre sus miembros. Este artículo tiene como objetivo revisar los principales factores psicosociales relacionados con el acceso, la integración y el desempeño de las personas con discapacidad en las organizaciones y sus implicaciones para el bienestar, la salud ocupacional y la calidad de vida laboral de este colectivo. A partir del modelo teórico propuesto por Stone y Colella¹ analizamos los resultados de las principales investigaciones sobre los factores macroorganizacionales e interpersonales y su relación con los riesgos psicosociales y su prevención. Finalmente, se ofrecen una serie de conclusiones y de propuestas de intervención e investigación futura dirigidas a la mejora de la salud ocupacional de los trabajadores con discapacidad.

  17. Fiber mediated receptor masking in non-infected bystander cells restricts adenovirus cell killing effect but promotes adenovirus host co-existence.

    Directory of Open Access Journals (Sweden)

    Johan Rebetz

    Full Text Available The basic concept of conditionally replicating adenoviruses (CRAD as oncolytic agents is that progenies generated from each round of infection will disperse, infect and kill new cancer cells. However, CRAD has only inhibited, but not eradicated tumor growth in xenograft tumor therapy, and CRAD therapy has had only marginal clinical benefit to cancer patients. Here, we found that CRAD propagation and cancer cell survival co-existed for long periods of time when infection was initiated at low multiplicity of infection (MOI, and cancer cell killing was inefficient and slow compared to the assumed cell killing effect upon infection at high MOI. Excessive production of fiber molecules from initial CRAD infection of only 1 to 2% cancer cells and their release prior to the viral particle itself caused a tropism-specific receptor masking in both infected and non-infected bystander cells. Consequently, the non-infected bystander cells were inefficiently bound and infected by CRAD progenies. Further, fiber overproduction with concomitant restriction of adenovirus spread was observed in xenograft cancer therapy models. Besides the CAR-binding Ad4, Ad5, and Ad37, infection with CD46-binding Ad35 and Ad11 also caused receptor masking. Fiber overproduction and its resulting receptor masking thus play a key role in limiting CRAD functionality, but potentially promote adenovirus and host cell co-existence. These findings also give important clues for understanding mechanisms underlying the natural infection course of various adenoviruses.

  18. Genetic and Molecular Epidemiological Characterization of a Novel Adenovirus in Antarctic Penguins Collected between 2008 and 2013.

    Directory of Open Access Journals (Sweden)

    Sook-Young Lee

    Full Text Available Antarctica is considered a relatively uncontaminated region with regard to the infectious diseases because of its extreme environment, and isolated geography. For the genetic characterization and molecular epidemiology of the newly found penguin adenovirus in Antarctica, entire genome sequencing and annual survey of penguin adenovirus were conducted. The entire genome sequences of penguin adenoviruses were completed for two Chinstrap penguins (Pygoscelis antarctica and two Gentoo penguins (Pygoscelis papua. The whole genome lengths and G+C content of penguin adenoviruses were found to be 24,630-24,662 bp and 35.5-35.6%, respectively. Notably, the presence of putative sialidase gene was not identified in penguin adenoviruses by Rapid Amplification of cDNA Ends (RACE-PCR as well as consensus specific PCR. The penguin adenoviruses were demonstrated to be a new species within the genus Siadenovirus, with a distance of 29.9-39.3% (amino acid, 32.1-47.9% in DNA polymerase gene, and showed the closest relationship with turkey adenovirus 3 (TAdV-3 in phylogenetic analysis. During the 2008-2013 study period, the penguin adenoviruses were annually detected in 22 of 78 penguins (28.2%, and the molecular epidemiological study of the penguin adenovirus indicates a predominant infection in Chinstrap penguin population (12/30, 40%. Interestingly, the genome of penguin adenovirus could be detected in several internal samples, except the lymph node and brain. In conclusion, an analysis of the entire adenoviral genomes from Antarctic penguins was conducted, and the penguin adenoviruses, containing unique genetic character, were identified as a new species within the genus Siadenovirus. Moreover, it was annually detected in Antarctic penguins, suggesting its circulation within the penguin population.

  19. Adenovirus serotype 7 associated with a severe lower respiratory tract disease outbreak in infants in Shaanxi Province, China

    Directory of Open Access Journals (Sweden)

    Xu Wenbo

    2011-01-01

    Full Text Available Abstract Background Pneumonia caused by adenovirus infection is usually severe especially with adenovirus serotype 7 commonly associated with lower respiratory tract disease outbreaks. We reported an outbreak of 70 cases of severe pneumonia with one death of infants in Shaanxi Province, China. Sampling showed adenovirus 7 (Ad7 as the primary pathogen with some co-infections. Results Two strains of adenovirus and two strains of enterovirus were isolated, the 21 pharynx swabs showed 14 positive amplifications for adenovirus; three co-infections with respiratory syncytial virus, two positive for rhinovirus, one positive for parainfluenza 3, and four negative. Adenovirus typing showed nine of the nine adenovirus positive samples were HAdV-7, three were HAdV-3 and two were too weak to perform sequencing. The entire hexon gene of adenovirus was sequenced and analyzed for the two adenovirus serotype 7 isolates, showing the nucleic acid homology was 99.8% between the two strains and 99.5% compared to the reference strain HAdV-7 (GenBank accession number AY769946. For the 21 acute phase serum samples from the 21 patients, six samples had positives results for ELISA detection of HAdV IgA, and the neutralization titers of the convalescent-phase samples were four times higher than those of the acute-phase samples in nine pairs. Conclusions We concluded adenovirus was the viral pathogen, primarily HAdV-7, with some co-infections responsible for the outbreak. This is the first report of an infant pneumonia outbreak caused by adenovirus serotype 7 in Shaanxi Province, China.

  20. Detection and Analysis of Six Lizard Adenoviruses by Consensus Primer PCR Provides Further Evidence of a Reptilian Origin for the Atadenoviruses

    OpenAIRE

    Wellehan, James F. X.; Johnson, April J.; Harrach, Balázs; Benkö, Mária; Pessier, Allan P.; Johnson, Calvin M.; Garner, Michael M.; Childress, April; Jacobson, Elliott R.

    2004-01-01

    A consensus nested-PCR method was designed for investigation of the DNA polymerase gene of adenoviruses. Gene fragments were amplified and sequenced from six novel adenoviruses from seven lizard species, including four species from which adenoviruses had not previously been reported. Host species included Gila monster, leopard gecko, fat-tail gecko, blue-tongued skink, Tokay gecko, bearded dragon, and mountain chameleon. This is the first sequence information from lizard adenoviruses. Phyloge...

  1. Pandemic Influenza Virus 2009 H1N1 and Adenovirus in a High Risk Population of Young Adults: Epidemiology, Comparison of Clinical Presentations, and Coinfection

    Science.gov (United States)

    2014-01-08

    a variety of pathogens. With the exception of the prior adenovirus vaccine era from 1980– 1996, adenoviruses have historically been the most common...administration of both live attenuated influenza and adenovirus vaccines , which could affect current trainee vaccine policies. In the meantime, concerns...change since the late 2011 reintroduction of adenovirus serotypes 4 and 7 vaccines in military trainees, or whether issues arise with concurrent

  2. Esteres metílicos de ácidos grasos como carburantes. Implicaciones económicas, ecológicas y energéticas

    Directory of Open Access Journals (Sweden)

    Gómez Herrera, Carlos

    1995-04-01

    Full Text Available Changes in the "Common Agricultural Policy" of the European Union have increased the rapeseed crops on "set-aside" lands remarkably. Rapeseed oils are transesterified with methanol to obtain fatty acid methyl esters. Blends of these esters with diesel oil are sold in petrol stations in different european countries like any other fuel.
    This informative article starts by reviewing basic characteristics of diesel fuel, and continues with an analysis of physical properties, chemical composition and present specifications for the rapeseed methyl esters. Subsequently several implications derived from the industrial development of this biofuel are discussed. These implications are economic (production, consumption, prices, subventions, tax exemptions, ecological (nearly zero emissions for whole cycle of carbon dioxide, other exhaust emissions from combustion, ecobalances, and energetic (complete evaluation of energy expenditure for production, energetic ratios.

    Los cambios en la "Common Agricultural Policy" de la Unión Europea han incrementado notablemente los cultivos de colza en los terrenos "setaside". El aceite de colza se transesterifica con metanol para obtener esteres metílicos de ácidos grasos del aceite de sus semillas. Mezclas de estos metilésteres con gasóleo se venden en gasolineras de diferentes países europeos como cualquier otro carburante.
    Este artículo de información comienza por una revisión de características básicas del gasóleo, seguida por un análisis de las propiedades físicas, la composición química y las especificaciones actuales de los metilésteres de colza. A continuación se discuten diversas implicaciones derivadas del desarrollo industrial de este biocombustible. Las mismas son económicas (producción, consumo, precios, subvenciones, exención de Impuestos, ecológicas (emisiones casi nulas para el ciclo completo del dióxido de carbono, otras emisiones procedentes de la combusti

  3. Límites de consistencia y sus implicaciones agrícolas en un suelo ultisol de sabana del estado Monagas, Venezuela

    Directory of Open Access Journals (Sweden)

    Américo Hossne

    2004-01-01

    Full Text Available El conocimiento de las propiedades físicas de los suelos agrícolas y bióticos proporciona información para el manejo de equipos agrícolas, procesos de compactación y la labranza convencional y conservacionista. A medida que la humedad edáfica aumenta, genera diversos estados del suelo; e.g. una menor interacción entre partículas adyacentes y un comportamiento que se aproxima al del líquido. El inicio de la sensibilidad de compactación, parece ser el límite plástico del suelo bajo una bien definida energía de compactación. El objetivo general, de este estudio fue caracterizar sistemáticamente, con las implicaciones de ingeniería posibles, los suelos agrícolas de sabana del Estado Monagas de Venezuela. Los objetivos específicos consistieron en determinar: a- los límites de Atterberg; y b- el índice de plasticidad y friabilidad. Para ello se utilizó los procedimientos de laboratorio de Mecánica de Suelos: ASTM D427-61 para el límite de contracción, ASTM D424-59 para el límite plástico y ASTM 423-66 para el límite líquido, de un suelo de sabana en muestras aleatorias a las profundidades de 0-15, 15-30, 30-45 y 45-60 cm. Se utilizó el análisis de regresión para interpretar la proporción de la variancia entre parámetros. Los resultados indicaron valores de: límite de contracción de 4,22–5,20%, límite plástico de 12,92–14,04%, límite líquido de 16,94–19,43%, índice de plasticidad de 3,59–5,78% e índice de friabilidad de 8,63–8,84%. Se concluye que los valores concuerdan con la bibliografía y el rango de uso agrícola se encuentra entre 8% y 15% para el suelo en estudio.

  4. On the mechanism of arginine requirement for adenovirus synthesis

    International Nuclear Information System (INIS)

    Plaat, D.; Weber, J.

    1979-01-01

    The effects of arginine deprivation on the synthesis and processing of viral proteins and the assembly of incomplete and complete virions were studied during infection with human adenovirus type 2. Arginine deprivation greatly reduced the synthesis of all viral proteins, particularly the precursor to core protein VII. The inhibition was completely reversible by the addition of arginine to the medium. Arginine deprivation between 7 and 20 hours post-infection inhibited the processing of PVII to VII, suggesting that PVII is not cleaved autocatalytically. The assembly of incomplete virions was sensitive to arginine deprivation only prior to 20 hours, while the assembly of complete virions was dependent on the continuous presence of arginine. This observation supports the hypothesis that incomplete virions are precursors of complete virions. The experiments on the PVII-specific endoprotease activity showed that arginine deprivation caused only slight reduction in the in vitro activity, although no activity was observed in vivo. The present results lead to the hypothesis that arginine deficiency inhibits the synthesis of a functional protein essential for virion maturation, other than the synthesis of processing of PVII. (author)

  5. Determination of the transforming activities of adenovirus oncogenes.

    Science.gov (United States)

    Speiseder, Thomas; Nevels, Michael; Dobner, Thomas

    2014-01-01

    The last 50 years of molecular biological investigations into human adenoviruses (Ads) have contributed enormously to our understanding of the basic principles of normal and malignant cell growth. Much of this knowledge stems from analyses of the Ad productive infection cycle in permissive host cells. Also, initial observations concerning the transforming potential of human Ads subsequently revealed decisive insights into the molecular mechanisms of the origins of cancer and established Ads as a model system for explaining virus-mediated transformation processes. Today it is well established that cell transformation by human Ads is a multistep process involving several gene products encoded in early transcription units 1A (E1A) and 1B (E1B). Moreover, a large body of evidence now indicates that alternative or additional mechanisms are engaged in Ad-mediated oncogenic transformation involving gene products encoded in early region 4 (E4) as well as epigenetic changes resulting from viral DNA integration. In particular, studies on the transforming potential of several E4 gene products have now revealed new pathways that point to novel general mechanisms of virus-mediated oncogenesis. In this chapter we describe in vitro and in vivo assays to determine the transforming and oncogenic activities of the E1A, E1B, and E4 oncoproteins in primary baby rat kidney cells, human amniotic fluid cells and athymic nude mice.

  6. Construction of an infectious clone of human adenovirus type 41.

    Science.gov (United States)

    Chen, Duo-Ling; Dong, Liu-Xin; Li, Meng; Guo, Xiao-Juan; Wang, Min; Liu, Xin-Feng; Lu, Zhuo-Zhuang; Hung, Tao

    2012-07-01

    Human adenovirus type 41 (HAdV-41) is well known for its fastidiousness in cell culture. To construct an infectious clone of HAdV-41, a DNA fragment containing the left and right ends of HAdV-41 as well as a kanamycin resistance gene and a pBR322 replication origin was excised from the previously constructed plasmid pAd41-GFP. Using homologous recombination, the plasmid pKAd41 was generated by co-transformation of the E. coli BJ5183 strain with this fragment and HAdV-41 genomic DNA. Virus was rescued from pKAd41-transfected 293TE7 cells, a HAdV-41 E1B55K-expressing cell line. The genomic integrity of the rescued virus was verified by restriction analysis and sequencing. Two fibers on the virion were confirmed by western blot. Immunofluorescence showed that more expression of the hexon protein could be found in 293TE7 cells than in 293 cells after HAdV-41 infection. The feature of non-lytic replication was preserved in 293TE7 cells, since very few progeny HAdV-41 viruses were released to the culture medium. These results show that pKAd41 is an effective infectious clone and suggest that the combination of pKAd41 and 293TE7 cells is an ideal system for virological study of HAdV-41.

  7. Oncolytic Replication of E1b-Deleted Adenoviruses

    Directory of Open Access Journals (Sweden)

    Pei-Hsin Cheng

    2015-11-01

    Full Text Available Various viruses have been studied and developed for oncolytic virotherapies. In virotherapy, a relatively small amount of viruses used in an intratumoral injection preferentially replicate in and lyse cancer cells, leading to the release of amplified viral particles that spread the infection to the surrounding tumor cells and reduce the tumor mass. Adenoviruses (Ads are most commonly used for oncolytic virotherapy due to their infection efficacy, high titer production, safety, easy genetic modification, and well-studied replication characteristics. Ads with deletion of E1b55K preferentially replicate in and destroy cancer cells and have been used in multiple clinical trials. H101, one of the E1b55K-deleted Ads, has been used for the treatment of late-stage cancers as the first approved virotherapy agent. However, the mechanism of selective replication of E1b-deleted Ads in cancer cells is still not well characterized. This review will focus on three potential molecular mechanisms of oncolytic replication of E1b55K-deleted Ads. These mechanisms are based upon the functions of the viral E1B55K protein that are associated with p53 inhibition, late viralmRNAexport, and cell cycle disruption.

  8. Taxonomy proposal for Old World monkey adenoviruses: characterisation of several non-human, non-ape primate adenovirus lineages.

    Science.gov (United States)

    Pantó, Laura; Podgorski, Iva I; Jánoska, Máté; Márkó, Orsolya; Harrach, Balázs

    2015-12-01

    A species classification regarding Old World monkey adenoviruses is proposed. We determined the nucleotide sequences of PCR-amplified fragments from the genes of the IVa2, DNA-dependent DNA polymerase, penton base, and hexon proteins from every simian adenovirus (SAdV) serotype that originated from Old World monkeys for which the full genome sequence had not yet been published. We confirmed that the majority of Old Word monkey SAdVs belong to two previously established species. Interestingly, one is the most recently established human AdV species, Human mastadenovirus G, which includes a single human virus, HAdV-52, as well as SAdV-1, -2, -7, -11, -12, and -15. The other approved species, Simian mastadenovirus A includes SAdV-3, -4, -6, -9, -10, -14, and -48. Several SAdVs (SAdV-5, -8, -49, -50) together with baboon AdV-1 and rhesus monkey AdV strains A1139, A1163, A1173, A1258, A1285, A1296, A1312, A1327 and A1335 have been proposed to be classified into an additional species, Simian mastadenovirus B. Another proposed species, Simian mastadenovirus C has been described for SAdV-19, baboon AdV-2/4 and -3. Our study revealed the existence of four additional AdV lineages. The corresponding new candidate species are Simian mastadenovirus D (for SAdV-13), Simian mastadenovirus E (for SAdV-16), Simian mastadenovirus F (for SAdV-17 and -18), and Simian mastadenovirus G (for SAdV-20). Several biological and genomic properties, such as the host origin, haemagglutination profile, number of fibre genes, and G+C content of the genome, strongly support this classification. Three SAdV strains originating from the American Type Culture Collection turned out to be mixtures of at least two virus types, either of the same species (SAdV-12 and -15 types from Human mastadenovirus G) or of two different species (SAdV-5 types from Simian mastadenovirus B and Human mastadenovirus G).

  9. Cancer-Targeted Oncolytic Adenoviruses for Modulation of the Immune System.

    Science.gov (United States)

    Cerullo, Vincenzo; Capasso, Cristian; Vaha-Koskela, Markus; Hemminki, Otto; Hemminki, Akseli

    2018-01-01

    Adenovirus is one of the most commonly used vectors for gene therapy and it is the first approved virus-derived drug for treatment of cancer. As an oncolytic agent, it can induce lysis of infected cells, but it can also engage the immune system, promoting activation and maturation of antigen- presenting cells (APCs). In essence, oncolysis combined with the associated immunostimulatory actions result in a "personalized in situ vaccine" for each patient. In order to take full advantage of these features, we should try to understand how adenovirus interacts with the immune system, what are the receptors involved in triggering subsequent signals and which kind of responses they elicit. Tackling these questions will give us further insight in how to manipulate adenovirus-mediated immune responses for enhancement of anti-tumor efficacy. In this review, we first highlight how oncolytic adenovirus interacts with the innate immune system and its receptors such as Toll-like receptors, nucleotide-binding and oligomerization domain (NOD)- like receptors and other immune sensors. Then we describe the effect of these interactions on the adaptive immune system and its cells, especially B and T lymphocytes. Finally, we summarize the most significant preclinical and clinical results in the field of gene therapy where researchers have engineered adenovirus to manipulate the host immune system by expressing cytokines and signalingmediators. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  10. Identification and Application of Neutralizing Epitopes of Human Adenovirus Type 55 Hexon Protein

    Directory of Open Access Journals (Sweden)

    Xingui Tian

    2015-10-01

    Full Text Available Human adenovirus type 55 (HAdV55 is a newly identified re-emergent acute respiratory disease (ARD pathogen with a proposed recombination of hexon gene between HAdV11 and HAdV14 strains. The identification of the neutralizing epitopes is important for the surveillance and vaccine development against HAdV55 infection. In this study, four type-specific epitope peptides of HAdV55 hexon protein, A55R1 (residues 138 to 152, A55R2 (residues 179 to 187, A55R4 (residues 247 to 259 and A55R7 (residues 429 to 443, were predicted by multiple sequence alignment and homology modeling methods, and then confirmed with synthetic peptides by enzyme-linked immunosorbent assay (ELISA and neutralization tests (NT. Finally, the A55R2 was incorporated into human adenoviruses 3 (HAdV3 and a chimeric adenovirus rAd3A55R2 was successfully obtained. The chimeric rAd3A55R2 could induce neutralizing antibodies against both HAdV3 and HAdV55. This current study will contribute to the development of novel adenovirus vaccine candidate and adenovirus structural analysis.

  11. Identification and Application of Neutralizing Epitopes of Human Adenovirus Type 55 Hexon Protein

    Science.gov (United States)

    Tian, Xingui; Ma, Qiang; Jiang, Zaixue; Huang, Junfeng; Liu, Qian; Lu, Xiaomei; Luo, Qingming; Zhou, Rong

    2015-01-01

    Human adenovirus type 55 (HAdV55) is a newly identified re-emergent acute respiratory disease (ARD) pathogen with a proposed recombination of hexon gene between HAdV11 and HAdV14 strains. The identification of the neutralizing epitopes is important for the surveillance and vaccine development against HAdV55 infection. In this study, four type-specific epitope peptides of HAdV55 hexon protein, A55R1 (residues 138 to 152), A55R2 (residues 179 to 187), A55R4 (residues 247 to 259) and A55R7 (residues 429 to 443), were predicted by multiple sequence alignment and homology modeling methods, and then confirmed with synthetic peptides by enzyme-linked immunosorbent assay (ELISA) and neutralization tests (NT). Finally, the A55R2 was incorporated into human adenoviruses 3 (HAdV3) and a chimeric adenovirus rAd3A55R2 was successfully obtained. The chimeric rAd3A55R2 could induce neutralizing antibodies against both HAdV3 and HAdV55. This current study will contribute to the development of novel adenovirus vaccine candidate and adenovirus structural analysis. PMID:26516903

  12. Innate Functions of Immunoglobulin M Lessen Liver Gene Transfer with Helper-Dependent Adenovirus

    Science.gov (United States)

    Unzu, Carmen; Morales-Kastresana, Aizea; Sampedro, Ana; Serrano-Mendioroz, Irantzu; Azpilikueta, Arantza; Ochoa, María Carmen; Dubrot, Juan; Martínez-Ansó, Eduardo

    2014-01-01

    The immune system poses obstacles to viral vectors, even in the first administration to preimmunized hosts. We have observed that the livers of B cell-deficient mice were more effectively transduced by a helper-dependent adenovirus serotype-5 (HDA) vector than those of WT mice. This effect was T-cell independent as shown in athymic mice. Passive transfer of the serum from adenovirus-naïve WT to Rag1KO mice resulted in a reduction in gene transfer that was traced to IgM purified from serum of adenovirus-naïve mice. To ascribe the gene transfer inhibition activity to either adenoviral antigen-specific or antigen-unspecific functions of IgM, we used a monoclonal IgM antibody of unrelated specificity. Both the polyclonal and the irrelevant monoclonal IgM inhibited gene transfer by the HDA vector to either cultured hepatocellular carcinoma cells or to the liver of mice in vivo. Adsorption of polyclonal or monoclonal IgMs to viral capsids was revealed by ELISAs on adenovirus-coated plates. These observations indicate the existence of an inborn IgM mechanism deployed against a prevalent virus to reduce early post-infection viremia. In conclusion, innate IgM binding to adenovirus serotype-5 capsids restrains gene-transfer and offers a mechanism to be targeted for optimization of vector dosage in gene therapy with HDA vectors. PMID:24465560

  13. Innate functions of immunoglobulin M lessen liver gene transfer with helper-dependent adenovirus.

    Directory of Open Access Journals (Sweden)

    Carmen Unzu

    Full Text Available The immune system poses obstacles to viral vectors, even in the first administration to preimmunized hosts. We have observed that the livers of B cell-deficient mice were more effectively transduced by a helper-dependent adenovirus serotype-5 (HDA vector than those of WT mice. This effect was T-cell independent as shown in athymic mice. Passive transfer of the serum from adenovirus-naïve WT to Rag1KO mice resulted in a reduction in gene transfer that was traced to IgM purified from serum of adenovirus-naïve mice. To ascribe the gene transfer inhibition activity to either adenoviral antigen-specific or antigen-unspecific functions of IgM, we used a monoclonal IgM antibody of unrelated specificity. Both the polyclonal and the irrelevant monoclonal IgM inhibited gene transfer by the HDA vector to either cultured hepatocellular carcinoma cells or to the liver of mice in vivo. Adsorption of polyclonal or monoclonal IgMs to viral capsids was revealed by ELISAs on adenovirus-coated plates. These observations indicate the existence of an inborn IgM mechanism deployed against a prevalent virus to reduce early post-infection viremia. In conclusion, innate IgM binding to adenovirus serotype-5 capsids restrains gene-transfer and offers a mechanism to be targeted for optimization of vector dosage in gene therapy with HDA vectors.

  14. Screening for adenoviruses in haematological neoplasia: High prevalence in mantle cell lymphoma.

    Science.gov (United States)

    Kosulin, Karin; Rauch, Margit; Ambros, Peter F; Pötschger, Ulrike; Chott, Andreas; Jäger, Ulrich; Drach, Johannes; Nader, Alexander; Lion, Thomas

    2014-02-01

    Human adenoviruses possess oncogenic capacity which is well documented in mammalian animal models, but their possible implication in human malignancy has remained enigmatic. Following primary infection, adenoviruses can persist in a latent state in lymphocytes where the virus is apparently able to evade immune surveillance. In the present study, we have employed a broad-spectrum adenovirus polymerase chain reaction (PCR) assay to systematically screen more than 200 diagnostic specimens of different lymphoid malignancies including acute lymphocytic leukaemia (n=50), chronic lymphocytic leukaemia (n=50), various types of malignant lymphoma (n=100) and multiple myeloma (n=11) for the presence of adenoviral sequences. While most entities analysed revealed negative findings in virtually all specimens tested, adenoviral DNA was detected in 15/36 (42%) mantle cell lymphomas investigated. The most prevalent adenoviral species detected was C, and less commonly B. Adenovirus-positive findings in patients with mantle cell lymphoma were made at different sites including bone marrow (n=7), intestine (n=5), lymph nodes (n=2) and tonsillar tissue (n=1). The presence of adenoviral sequences identified by PCR was confirmed in individual cells by fluorescence in-situ hybridisation (FISH). The frequent observation of adenoviruses in mantle cell lymphoma is intriguings, and raises questions about their possible involvement in the pathogenesis of this lymphoid malignancy. Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. CALIDAD DE VIDA Y ESTRATEGIAS DE AFRONTAMIENTO EN PACIENTES CON INSUFICIENCIA RENAL CRÓNICA SOMETIDOS A HEMODIÁLISIS, DIÁLISIS PERITONEAL Y TRASPLANTE RENAL

    Directory of Open Access Journals (Sweden)

    Cesar Armando Rey Anacona

    2008-07-01

    Full Text Available Se comparó la calidad de vida y las estrategias de afrontamiento en 120 pacientes con Insuficiencia Renal Crónica sometidos a hemodiálisis, diálisis peritoneal o trasplante renal, teniendo en cuenta la edad, sexo, escolaridad, estado civil y ocupación. Los instrumentos utilizados fueron una ficha sociodemográfica, la prueba de calidad de vida WHOQOL-100 (Organización Mundial de la Salud, 1998 y el Cuestionario de Estrategias de Afrontamiento (Lazarus & Folkman, 1985. Se encontró que los pacientes en tratamiento de trasplante reportaban mayor calidad de vida y mejores estrategias de afrontamiento en comparación con los otros tratamientos, siendo mayores las puntuaciones en personas con edad de transición entre adultez media y tardía, mujeres, con estudios universitarios, casados y empleados. Se discuten las implicaciones de estos hallazgos.

  16. Replication of type 5 adenovirus promotes middle ear infection by Streptococcus pneumoniae in the chinchilla model of otitis media

    Science.gov (United States)

    Murrah, Kyle A.; Turner, Roberta L.; Pang, Bing; Perez, Antonia C.; Reimche, Jennifer L.; King, Lauren B.; Wren, John; Gandhi, Uma; Swords, W. Edward; Ornelles, David A.

    2015-01-01

    Adenoviral infection is a major risk factor for otitis media. We hypothesized that adenovirus promotes bacterial ascension into the middle ear through the disruption of normal function in the Eustachian tubes due to inflammation-induced changes. An intranasal infection model of the chinchilla was used to test the ability of type 5 adenovirus to promote middle ear infection by Streptococcus pneumoniae. The hyperinflammatory adenovirus mutant dl327 and the nonreplicating adenovirus mutant H5wt300ΔpTP were used to test the role of inflammation and viral replication, respectively, in promotion of pneumococcal middle ear infection. Precedent infection with adenovirus resulted in a significantly greater incidence of middle ear disease by S. pneumoniae as compared to nonadenovirus infected animals. Infection with the adenovirus mutant dl327 induced a comparable degree of bacterial ascension into the middle ear as did infection with the wild-type virus. By contrast, infection with the nonreplicating adenovirus mutant H5wt300ΔpTP resulted in less extensive middle ear infection compared to the wild-type adenovirus. We conclude that viral replication is necessary for adenoviral-induced pneumococcal middle ear disease. PMID:25251686

  17. Immunogenicity of heterologous recombinant adenovirus prime-boost vaccine regimens is enhanced by circumventing vector cross-reactivity

    NARCIS (Netherlands)

    Thorner, Anna R.; Lemckert, Angelique A. C.; Goudsmit, Jaap; Lynch, Diana M.; Ewald, Bonnie A.; Denholtz, Matthew; Havenga, Menzo J. E.; Barouch, Dan H.

    2006-01-01

    The high prevalence of preexisting immunity to adenovirus serotype 5 (Ad5) in human populations has led to the development of recombinant adenovirus (rAd) vectors derived from rare Ad serotypes as vaccine candidates for human immunodeficiency virus type 1 and other pathogens. Vaccine vectors have

  18. Fiber-chimeric adenoviruses expressing fibers from serotype 16 and 50 improve gene transfer to human pancreatic adenocarcinoma

    NARCIS (Netherlands)

    Kuhlmann, K.F.D.; Geer, M.A. van; Bakker, C.T.; Dekker, J.E.M.; Havenga, M.J.E.; Oude Elferink, R.P.J.; Gouma, D.J.; Bosma, P.J.; Wesseling, J.G.

    2009-01-01

    Survival of patients with pancreatic cancer is poor. Adenoviral (Ad) gene therapy employing the commonly used serotype 5 reveals limited transduction efficiency due to the low amount of coxsackie-adenovirus receptor on pancreatic cancer cells. To identify fiber-chimeric adenoviruses with improved

  19. BS69 : A novel adenovirus E1A-associated protein that inhibits E1A transactivation

    NARCIS (Netherlands)

    Hateboer, G.; Gennissen, A.M.C.; Ramos, Y.F.M.; Kerkhoven, R.; Sonntag-Buck, V.; Stunnenberg, H.G.; Bernards, R.A.

    1995-01-01

    The adenovirus ElA gene products are nuclear phosphoproteins that can transactivate the other adenovirus early genes as well as several cellular genes, and can transform primary rodent cells in culture. Transformation and transactivation by ElA proteins is most likely to be mediated through

  20. Los valores éticos y sus implicaciones en la gestión administrativa

    Directory of Open Access Journals (Sweden)

    Edith Rodriguez

    2016-11-01

    Full Text Available El objetivo de esta investigación es determinar los valores éticos que poseen los estudiantes de cuarto curso de Administración de Empresas de la Universidad de Guayaquil y sus implicaciones en la Gestión Administrativa. El problema se genera a partir de las observaciones realizadas por la autora de este artículo, a los estudiantes de la Carrera de Gestión Empresarial de la Facultad de Ciencias Administrativas de la Universidad de Guayaquil en los procesos de clase. La metodología utilizada corresponde a un estudio de campo de tipo descriptivo y explicativo, se utilizó un instrumento de recolección de datos tipo encuesta fue aplicado a una población constituida por 120 estudiantes de la carrera de gestión empresarial. Los resultados del estudio  evidenciaron un bajo nivel en el cumplimiento de los códigos de ética por parte de los estudiantes, la relación entre las variables de investigación resultó ser positiva muy débil, lo que significa que mientras el estudiante mejore el cumplimiento de la ética en su actividad áulica, se evidenciará en estos un mejor  desempeño profesional. La investigación concluye proponiendo una serie de estrategias a fin de proporcionar al estudiante la posibilidad de participación a cursos de capacitación, talleres o charlas donde se compartan conocimientos entre los integrantes de la universidad, planificados y organizados por la carrera.

  1. Redirecting adenovirus tropism by genetic, chemical, and mechanical modification of the adenovirus surface for cancer gene therapy.

    Science.gov (United States)

    Yoon, A-Rum; Hong, Jinwoo; Kim, Sung Wan; Yun, Chae-Ok

    2016-06-01

    Despite remarkable advancements, clinical evaluations of adenovirus (Ad)-mediated cancer gene therapies have highlighted the need for improved delivery and targeting. Genetic modification of Ad capsid proteins has been extensively attempted. Although genetic modification enhances the therapeutic potential of Ad, it is difficult to successfully incorporate extraneous moieties into the capsid and the engineering process is laborious. Recently, chemical modification of the Ad surface with nanomaterials and targeting moieties has been found to enhance Ad internalization into the target by both passive and active mechanisms. Alternatively, external stimulus-mediated targeting can result in selective accumulation of Ad in the tumor and prevent dissemination of Ad into surrounding nontarget tissues. In the present review, we discuss various genetic, chemical, and mechanical engineering strategies for overcoming the challenges that hinder the therapeutic efficacy of Ad-based approaches. Surface modification of Ad by genetic, chemical, or mechanical engineering strategies enables Ad to overcome the shortcomings of conventional Ad and enhances delivery efficiency through distinct and unique mechanisms that unmodified Ad cannot mimic. However, although the therapeutic potential of Ad-mediated gene therapy has been enhanced by various surface modification strategies, each strategy still possesses innate limitations that must be addressed, requiring innovative ideas and designs.

  2. Adenovirus Particles that Display the Plasmodium falciparum Circumsporozoite Protein NANP Repeat Induce Sporozoite-Neutralizing Antibodies in Mice

    Science.gov (United States)

    Palma, Christopher; Overstreet, Michael G.; Guedon, Jean-Marc; Hoiczyk, Egbert; Ward, Cameron; Karen, Kasey A.; Zavala, Fidel; Ketner, Gary

    2011-01-01

    Adenovirus particles can be engineered to display exogenous peptides on their surfaces by modification of viral capsid proteins, and particles that display pathogen-derived peptides can induce protective immunity. We constructed viable recombinant adenoviruses that display B-cell epitopes from the Plasmodium falciparum circumsporozoite protein (PfCSP) in the major adenovirus capsid protein, hexon. Recombinants induced high-titer antibodies against CSP when injected intraperitoneally into mice. Serum obtained from immunized mice recognized both recombinant PfCSP protein and P. falciparum sporozoites, and neutralized P. falciparum sporozoites in vitro. Replicating adenovirus vaccines have provided economical protection against adenovirus disease for over three decades. The recombinants described here may provide a path to an affordable malaria vaccine in the developing world. PMID:21199707

  3. The presence of enterovirus, adenovirus, and parvovirus B19 in myocardial tissue samples from autopsies

    DEFF Research Database (Denmark)

    Nielsen, Trine Skov; Hansen, Jakob; Nielsen, Lars Peter

    2014-01-01

    of adenovirus, enterovirus, and parvovirus B19 (PVB) in myocardial autopsy samples from myocarditis related deaths and in non-inflamed control hearts in an effort to clarify their significance as the causes of myocarditis in a forensic material. METHODS: We collected all autopsy cases diagnosed with myocarditis...... from 1992 to 2010. Eighty-four suicidal deaths with morphologically normal hearts served as controls. Polymerase chain reaction was used for the detection of the viral genomes (adenovirus, enterovirus, and PVB) in myocardial tissue specimens. The distinction between acute and persistent PVB infection...... was made by the serological determination of PVB-specific immunoglobulins M and G. RESULTS: PVB was detected in 33 of 112 (29 %) myocarditis cases and 37 of 84 (44 %) control cases. All of the samples were negative for the presence of adenovirus and enterovirus. Serological evidence of an acute PVB...

  4. Protection of non-human primates against rabies with an adenovirus recombinant vaccine

    International Nuclear Information System (INIS)

    Xiang, Z.Q.; Greenberg, L.; Ertl, H.C.; Rupprecht, C.E.

    2014-01-01

    Rabies remains a major neglected global zoonosis. New vaccine strategies are needed for human rabies prophylaxis. A single intramuscular immunization with a moderate dose of an experimental chimpanzee adenovirus (Ad) vector serotype SAd-V24, also termed AdC68, expressing the rabies virus glycoprotein, resulted in sustained titers of rabies virus neutralizing antibodies and protection against a lethal rabies virus challenge infection in a non-human primate model. Taken together, these data demonstrate the safety, immunogenicity, and efficacy of the recombinant Ad-rabies vector for further consideration in human clinical trials. - Highlights: • Pre-exposure vaccination with vaccine based on a chimpanzee derived adenovirus protects against rabies. • Protection is sustained. • Protection is achieved with single low-dose of vaccine given intramuscularly. • Protection is not affected by pre-existing antibodies to common human serotypes of adenovirus

  5. Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes

    International Nuclear Information System (INIS)

    Hyypiae, T.

    1985-01-01

    The presence of adenovirus DNA in clinical specimens was analyzed by nucleic acid hybridization assays by both radioactive and enzymatic detection systems. The sensitivity of the hybridization tests was in the range of 10 to 100 pg of homologous adenovirus DNA. Minimal background was noticed with unrelated viral and nonviral DNA. Twenty-four nasopharyngeal mucus aspirate specimens, collected from children with acute respiratory infection, were assayed in the hybridization tests and also by an enzyme immunoassay for adenovirus hexon antigen which was used as a reference test. Sixteen specimens positive by the enzyme immunoassay also were positive in the two nucleic acid hybridization tests, and the remaining eight specimens were negative in all of the tests. The results indicate that nucleid acid hybridization tests with both radioactive and nonradioactive probes can be used for diagnosis of microbial infections

  6. Protection of non-human primates against rabies with an adenovirus recombinant vaccine

    Energy Technology Data Exchange (ETDEWEB)

    Xiang, Z.Q. [The Wistar Institute of Anatomy and Biology, Philadelphia, PA (United States); Greenberg, L. [Centers for Disease Control and Prevention, Atlanta, GA (United States); Ertl, H.C., E-mail: ertl@wistar.upenn.edu [The Wistar Institute of Anatomy and Biology, Philadelphia, PA (United States); Rupprecht, C.E. [The Global Alliance for Rabies Control, Manhattan, KS (United States); Ross University School of Veterinary Medicine, Basseterre (Saint Kitts and Nevis)

    2014-02-15

    Rabies remains a major neglected global zoonosis. New vaccine strategies are needed for human rabies prophylaxis. A single intramuscular immunization with a moderate dose of an experimental chimpanzee adenovirus (Ad) vector serotype SAd-V24, also termed AdC68, expressing the rabies virus glycoprotein, resulted in sustained titers of rabies virus neutralizing antibodies and protection against a lethal rabies virus challenge infection in a non-human primate model. Taken together, these data demonstrate the safety, immunogenicity, and efficacy of the recombinant Ad-rabies vector for further consideration in human clinical trials. - Highlights: • Pre-exposure vaccination with vaccine based on a chimpanzee derived adenovirus protects against rabies. • Protection is sustained. • Protection is achieved with single low-dose of vaccine given intramuscularly. • Protection is not affected by pre-existing antibodies to common human serotypes of adenovirus.

  7. Intraductal delivery of adenoviruses targets pancreatic tumors in transgenic Ela-myc mice and orthotopic xenografts.

    Science.gov (United States)

    José, Anabel; Sobrevals, Luciano; Miguel Camacho-Sánchez, Juan; Huch, Meritxell; Andreu, Núria; Ayuso, Eduard; Navarro, Pilar; Alemany, Ramon; Fillat, Cristina

    2013-01-01

    Gene-based anticancer therapies delivered by adenoviruses are limited by the poor viral distribution into the tumor. In the current work we have explored the feasibility of targeting pancreatic tumors through a loco-regional route. We have taken advantage of the ductal network in the pancreas to retrogradelly inject adenoviruses through the common bile duct in two different mouse models of pancreatic carcinogenesis: The transgenic Ela-myc mice that develop mixed neoplasms displaying both acinar-like and duct-like neoplastic cells affecting the whole pancreas; and mice bearing PANC-1 and BxPC-3 orthotopic xenografts that constitute a model of localized human neoplastic tumors. We studied tumor targeting and the anticancer effects of newly thymidine kinase-engineered adenoviruses both in vitro and in vivo, and conducted comparative studies between intraductal or intravenous administration. Our data indicate that the intraductal delivery of adenovirus efficiently targets pancreatic tumors in the two mouse models. The in vivo application of AduPARTKT plus ganciclovir (GCV) treatment induced tumor regression in Ela-myc mice. Moreover, the intraductal injection of ICOVIR15-TKT oncolytic adenoviruses significantly improved mean survival of mice bearing PANC-1 and BxPC-3 pancreatic xenografts from 30 to 52 days and from 20 to 68 days respectively (p less than 0.0001) when combined with GCV. Of notice, both AduPARTKT and ICOVIR15-TKT antitumoral responses were stronger by ductal viral application than intravenously, in line with the 38-fold increase in pancreas transduction observed upon ductal administration. In summary our data show that cytotoxic adenoviruses retrogradelly injected to the pancreas can be a feasible approach to treat localized pancreatic tumors.

  8. Metabolic flux profiling of MDCK cells during growth and canine adenovirus vector production

    OpenAIRE

    Nuno Carinhas; Daniel A. M. Pais; Alexey Koshkin; Paulo Fernandes; Ana S. Coroadinha; Manuel J. T. Carrondo; Paula M. Alves; Ana P. Teixeira

    2016-01-01

    Canine adenovirus vector type 2 (CAV2) represents an alternative to human adenovirus vectors for certain gene therapy applications, particularly neurodegenerative diseases. However, more efficient production processes, assisted by a greater understanding of the effect of infection on producer cells, are required. Combining [1,2-13C]glucose and [U-13C]glutamine, we apply for the first time 13C-Metabolic flux analysis (13C-MFA) to study E1-transformed Madin-Darby Canine Kidney (MDCK) cells meta...

  9. Future prospects for the development of cost-effective Adenovirus vaccines

    DEFF Research Database (Denmark)

    Fougeroux, Cyrielle; Holst, Peter J

    2017-01-01

    -vectored vaccine technology with a focus on adenoviral-based vaccines. Adenovirus (Ad) vaccines have proven to be efficient in military vaccinations against Ad4 and Ad7 and as highly efficient vectored vaccines against rabies. The question of how other adenovirus-based vaccines can become as efficient...... as the rabies vaccine is the underlying theme in this review. Here, we will first give an overview of the basic properties of vectored vaccines, followed by an introduction to the characteristics of adenoviral vectors and previously tested modifications of the vector backbone and expression cassettes...

  10. Detection and analysis of six lizard adenoviruses by consensus primer PCR provides further evidence of a reptilian origin for the atadenoviruses.

    Science.gov (United States)

    Wellehan, James F X; Johnson, April J; Harrach, Balázs; Benkö, Mária; Pessier, Allan P; Johnson, Calvin M; Garner, Michael M; Childress, April; Jacobson, Elliott R

    2004-12-01

    A consensus nested-PCR method was designed for investigation of the DNA polymerase gene of adenoviruses. Gene fragments were amplified and sequenced from six novel adenoviruses from seven lizard species, including four species from which adenoviruses had not previously been reported. Host species included Gila monster, leopard gecko, fat-tail gecko, blue-tongued skink, Tokay gecko, bearded dragon, and mountain chameleon. This is the first sequence information from lizard adenoviruses. Phylogenetic analysis indicated that these viruses belong to the genus Atadenovirus, supporting the reptilian origin of atadenoviruses. This PCR method may be useful for obtaining templates for initial sequencing of novel adenoviruses.

  11. Outbreak of epidemic keratoconjunctivitis caused by adenovirus in medical residents.

    Science.gov (United States)

    Melendez, Carlos Pantoja; Florentino, Margarita Matias; Martinez, Irma Lopez; Lopez, Herlinda Mejia

    2009-01-01

    The present work documents an outbreak of epidemic keratoconjunctivitis among ophthalmology residents, its influence in the presentation of the community cases, the use of molecular techniques for its diagnosis, and the implementation of successful control measures for its containment. Isolation of the etiologic agent was achieved using cultured African green monkey kidney epithelial cells (VERO). Through molecular tests, such as polymerase chain reaction (PCR) and DNA sequencing, the genotype of the isolated virus was identified. The sequences obtained were aligned with data reported in the NCBI GenBank. A scheme of outbreak control measures was designed to enforce correct sanitary measures in the clinic. The statistical program, Epi info 2002, and openepi were used to determine the attack rate. The Excel Microsoft program was used to elaborate the endemic channel. Nine of the ten samples studied were isolated from the culture and identified by Adenovirus-specifc PCR. Sequencing allowed identification of Ad8 as the agent responsible for the outbreak. The attack rate was 24.39 cases per 100. The epidemic curve allowed identification of a disseminated source in the Institute of Ophthalmology "Conde de Valenciana." It was not possible to calculate the incubation periods among the cases. The endemic channel showed the presence of an epidemic keratoconjunctivitis among the patients that had been cared for at the out-patient services of the institute. One outbreak of a disseminated source caused by Ad8 was detected in the institute among its medical residents, probably associated with relaxation of the habitual sanitary measures during an epidemic of hemorrhagic conjunctivitis among the patients cared for at the institute. The proposed scheme to control the outbreak allowed for its containment and controlled the epidemic of associated cases.

  12. Paciente con esquizofrenia tratado con ziprasidona + clozapina

    Directory of Open Access Journals (Sweden)

    Pol Yanguas E.

    2013-05-01

    Full Text Available P es un paciente diagnosticado de esquizofrenia, sigue en un piso tutelado un programa de rehabilitación, está medicado con clozapina 500 mg/día y ziprasidona 280 mg/ día. Padece hipercolesterolemia, tabaquismo y sus hábitos alimenticios no son buenos. La medicación que utiliza desde 2007 hasta ahora se refleja en la tabla 1. El último tratamiento se le introdujo el 7 de agosto de 2012, habiendo presentado un electro cardiograma (ECG normal, pero con ligera taquicardia ventricular y prolactinemia de 44,8 ng/ml (valores normales: 2-18 ng/ml.

  13. Key Role of the Scavenger Receptor MARCO in Mediating Adenovirus Infection and Subsequent Innate Responses of Macrophages.

    Science.gov (United States)

    Maler, Mareike D; Nielsen, Peter J; Stichling, Nicole; Cohen, Idan; Ruzsics, Zsolt; Wood, Connor; Engelhard, Peggy; Suomalainen, Maarit; Gyory, Ildiko; Huber, Michael; Müller-Quernheim, Joachim; Schamel, Wolfgang W A; Gordon, Siamon; Jakob, Thilo; Martin, Stefan F; Jahnen-Dechent, Willi; Greber, Urs F; Freudenberg, Marina A; Fejer, György

    2017-08-01

    The scavenger receptor MARCO is expressed in several subsets of naive tissue-resident macrophages and has been shown to participate in the recognition of various bacterial pathogens. However, the role of MARCO in antiviral defense is largely unexplored. Here, we investigated whether MARCO might be involved in the innate sensing of infection with adenovirus and recombinant adenoviral vectors by macrophages, which elicit vigorous immune responses in vivo Using cells derived from mice, we show that adenovirus infection is significantly more efficient in MARCO-positive alveolar macrophages (AMs) and in AM-like primary macrophage lines (Max Planck Institute cells) than in MARCO-negative bone marrow-derived macrophages. Using antibodies blocking ligand binding to MARCO, as well as gene-deficient and MARCO-transfected cells, we show that MARCO mediates the rapid adenovirus transduction of macrophages. By enhancing adenovirus infection, MARCO contributes to efficient innate virus recognition through the cytoplasmic DNA sensor cGAS. This leads to strong proinflammatory responses, including the production of interleukin-6 (IL-6), alpha/beta interferon, and mature IL-1α. These findings contribute to the understanding of viral pathogenesis in macrophages and may open new possibilities for the development of tools to influence the outcome of infection with adenovirus or adenovirus vectors. IMPORTANCE Macrophages play crucial roles in inflammation and defense against infection. Several macrophage subtypes have been identified with differing abilities to respond to infection with both natural adenoviruses and recombinant adenoviral vectors. Adenoviruses are important respiratory pathogens that elicit vigorous innate responses in vitro and in vivo The cell surface receptors mediating macrophage type-specific adenovirus sensing are largely unknown. The scavenger receptor MARCO is expressed on some subsets of naive tissue-resident macrophages, including lung alveolar macrophages

  14. Development of a nasal adenovirus-based vaccine: Effect of concentration and formulation on adenovirus stability and infectious titer during actuation from two delivery devices.

    Science.gov (United States)

    Renteria, Sandra S; Clemens, Courtney C; Croyle, Maria A

    2010-02-25

    A nasal adenovirus-based vaccine is under development. To determine if aggregation occurs during vaccination, infectious titer (limiting dilution) and capsid integrity (dynamic light scattering) were assessed after extrusion of a model vector from two intranasal delivery devices. Preparations of 2.5x10(12) and 1.25x10(11) virus particles (vp)/ml were studied. Virus aggregated ( approximately 10%) in the multi-dose vessel. Virus titer dropped by one log. Virus in the unit-dose device aggregated ( approximately 1%). Titer remained unchanged. Aggregation was concentration dependent. Formulations prevented aggregation during actuation, freeze-thaw and long-term storage. The device, formulation and dose may significantly influence aggregation and potency of any nasal adenovirus 5-based vaccine. Copyright 2009 Elsevier Ltd. All rights reserved.

  15. Efficacy of severe acute respiratory syndrome vaccine based on a nonhuman primate adenovirus in the presence of immunity against human adenovirus.

    Science.gov (United States)

    Zhi, Yan; Figueredo, Joanita; Kobinger, Gary P; Hagan, Heather; Calcedo, Roberto; Miller, James R; Gao, Guangping; Wilson, James M

    2006-05-01

    Replication-deficient human adenovirus type 5 (AdH5) vectors can induce strong transgene product-specific cellular and humoral responses. However, many adult humans have neutralizing antibodies (NAbs) against AdH5 as a result of natural infection with this virus. Therefore, a chimpanzee adenovirus C7 (AdC7) vector was developed to circumvent interference by preexisting immunity to AdH5. This study evaluated the impact of preexisting immunity to human adenovirus on the efficacy of adenovirus-based vaccines against the coronavirus that causes severe acute respiratory syndrome (SARS-CoV). Efficacy was assessed after intramuscular injection of the vector into mice and was measured as the frequency of SARS-CoV-specific T cells and NAbs against SARS-CoV. Immunogenicity of the AdH5-based vaccine was significantly attenuated or completely abolished when the preexisting anti-AdH5 NAb titer was higher than 40. Because 27% of human serum samples from the United States tested so far have an anti-AdH5 NAb titer higher than 40, our results suggested that a significant percentage of humans with preexisting anti-AdH5 immunity would not be candidates for vaccination with an AdH5-based genetic vaccine. In contrast, preexisting anti-AdH5 NAbs have a minimal effect on the potency of the AdC7-based genetic vaccine. Taken together, our studies warrant the further development of AdC7 as a vaccine carrier for human trials.

  16. Detección de linfocitos T citotóxicos específicos anti-53 en pacientes con cáncer de mama

    OpenAIRE

    Álvarez Busto, Iñaki; Tres Sánchez, Alejandro; Mayordomo Cámara, José Ignacio

    2009-01-01

    El gen P53 es el más frecuentemente mutado en tumores humanos ( 50%). Su proteina producto, la proteina P53, se acumula cuando sufre una mutación. Esta circunstancia posibilita su presentación al sistema inmunológico y el desarrollo de una respuesta celular específica medida por linfocitos T CD8. En este trabajo por primera vez, ha sido posible desarrollar la existencia de una respuesta inmune celular específica "in vivo" en pacientes con cancer de mama. Las implicaciones de este hallazgo par...

  17. Don Quijote y los eruditos: Sobre una polémica crítica y sus implicaciones metacríticas

    OpenAIRE

    Pardo García, Pedro Javier

    2000-01-01

    [ES]Este trabajo trata sobre Don Quijote y los eruditos: una polémica crítica y sus implicaciones metacríticas. [EN]This paper deals with Don Quixote and scholars: a critical controversy and its implications metacritica.

  18. Evaluación de la conducta emprendedora en estudiantes universitarios: implicaciones para el diseño de programas académicos

    Directory of Open Access Journals (Sweden)

    Ana Lanero Carrizo

    2011-06-01

    Full Text Available Este trabajo parte de una revisión general de la iniciativa empresarial de los jóvenes españoles para proponer un modelo sociocognitivo del comportamiento emprendedor y sus desencadenantes durante la etapa universitaria. En la validación de dicho modelo, se describen los resultados de un estudio de autoinforme llevado a cabo con una muestra representativa de 400 estudiantes de la Universidad de León. Los datos recabados fueron tratados estadísticamente a través de análisis factorial exploratorio y análisis multivariado de la varianza (MANOVA. Los resultados obtenidos permitieron identificar cinco componentes en el estudio de la conducta emprendedora de los encuestados, en términos de autoeficacia emprendedora, expectativas de resultados extrínsecos e intrínsecos, intereses emprende-dores y elección de carrera emprendedora. En general, los estudiantes de titulaciones Socio-Jurídicas y Técnicas tuvieron puntuaciones medias más altas en dichas dimensiones que sus iguales de otras ramas académicas. Las implicaciones de tales resultados para el diseño de programas académicos adaptados al EEES son discutidas.This paper presents a general review of entrepreneurship in Spanish young people to propose a social cognitive model of entrepreneurial behaviour and its triggers in university contexts. To validate the model, we describe the results from a study carried out with a sample of 400 undergraduates at the University of León, in Spain. Data was analyzed by exploratory factor analysis and multivariate analysis of variance (MANOVA. Findings identified five components in the study of participants’ entrepreneurial behaviour, particularly, self-efficacy, extrinsic and intrinsic outcome expectations, entrepreneurial interests and career choice. In general, students within Social and Technical degrees displayed higher mean scores in those variables than undergraduates in other academic areas. Implications of these results for the design

  19. Certificación energética de edificios en España y sus implicaciones económicas

    Directory of Open Access Journals (Sweden)

    Ruá, M. J.

    2012-09-01

    Full Text Available Under the European Energy Performance of Buildings Directive, the Member States must develop methods to certify energy efficiency of buildings. Calener-VYP v1.0 is the only Spanish official tool to certify a high energy efficiency performance of residential buildings, through the general option. It has two indicators implemented, CO2 emissions and primary energy consumption. However, in the process of energy rating a building, only the CO2 emissions indicator is considered. The economic consequences of this are studied, by means of simulating real properties with different construction assemblies and building services in the twelve Spanish climatic zones and calculating their costs. The results show that having a better energy rate in Calener VYP does not necessarily entail a lower consumption cost. Therefore, it is necessary to include the annual primary energy consumption indicator, because its absence involves that costs cannot be used as an argument when promoting energy efficiency among users.

    Bajo la Directiva Europea sobre eficiencia energética de los edificios, los Estados Miembros deben desarrollar procedimientos para certificar energéticamente los edificios. Calener-VYP v1.0 es la única herramienta oficial española para certificar una elevada aptitud en eficiencia energética de edificios residenciales, por el método general. Tiene dos indicadores implementados, emisiones de CO2 y consumo de energía primaria. Sin embargo, en el proceso de otorgar la calificación energética, solamente se consideran las emisiones de CO2. Las implicaciones económicas de esto se estudian simulando viviendas con diferentes características constructivas e instalaciones en las doce zonas climáticas españolas y calculando sus costes. Los resultados muestran que una mejor calificación energética en Calener-VYP no implica necesariamente un menor coste de consumo energético. Por tanto, es necesario

  20. Implicaciones de la zona Ossa-Morena y dominios equivalentes en el modelo geodinámico de la Cadena Hercinica europea

    Directory of Open Access Journals (Sweden)

    Castro, A.

    1987-08-01

    Full Text Available The Ossa-Morena zone (OMZ has some peculiar features clearly different from those of the other inner domains in the Iberic Hercynian chain. It is a continental domain (microcontinent ? attached, from Lower Paleozoic times, to the continental crust of the Central Iberian zone (CIZ. During the Hercynian collision (Upper Paleozoic the OMZ behaved in a way different form that of adjacent areas (CIZ due to the peculiar features (Iess thicknees and pre-Hercynian evolution (Ordovician rifting. The OMZ, and their equivalent domains in the Iberian peninsula as well as in European chain, can have strong implications on the evolution of the Hercynian orogene. Part of the OMZ crust (and equivalent domains, together with the oceanic crust developed within it during the Ordovician rifting, was obduced over the inner domains (CIZ in areas of frontal collision (e.g. NW Iberian peninsula and French Massif Central and strongly stretched in areas of oblique collision (e.g. OMZ of SW Iberian peninsula and North Armorican domains.La zona Ossa-Morena (ZOM del hercínico peninsular posee unas características peculiares que la diferencian netamente de los otros dominios internos de la Cadena. Se trata de un dominio cortical (microcontinente ? adosado a la corteza de la Zona Centro-Ibérica (ZCI desde el Paleozoico Inferior. Durante la colisión continental hercínica (Palezoico Superior la ZOM responde de diferente forma que las zonas adyacentes (ZCI debido a su diferente constitución (menor espesor y peculiar evolución prehercínica (rifting ordovícico. Esta zona y sus equivalentes en otros dominios del hercínico peninsular y europeo, tienen fuertes implicaciones en el desarrollo del orógeno hercínico. Parte de la corteza continental de la ZOM fue, junto con la corteza oceánica desarrollada localmente en su interior durante el rifting ordovícido, obducida sobre los dominios internos (ZCI en zonas de colisión frontal (e.g. NW peninsular y Macizo C

  1. PDGF-receptor beta-targeted adenovirus redirects gene transfer from hepatocytes to activated stellate cells

    NARCIS (Netherlands)

    Schoemaker, Marieke H.; Rots, Marianne G.; Beljaars, Leonie; Ypma, Arjen Y.; Jansen, Peter L. M.; Poelstra, Klaas; Moshage, Albert; Haisma, Hidde J.

    2008-01-01

    Chronic liver damage may lead to liver fibrosis. In this process, hepatic activated stellate cells are the key players. Thus, activated stellate cells are attractive targets for antifibrotic gene therapy. Recombinant, adenovirus is a promising vehicle for delivering therapeutic genes to liver cells.

  2. Sequential and Simultaneous Applications of UV and Chlorine for Adenovirus Inactivation.

    Science.gov (United States)

    Rattanakul, Surapong; Oguma, Kumiko; Takizawa, Satoshi

    2015-09-01

    Adenoviruses are water-borne human pathogens with high resistance to UV disinfection. Combination of UV treatment and chlorination could be an effective approach to deal with adenoviruses. In this study, human adenovirus 5 (HAdV-5) was challenged in a bench-scale experiment by separate applications of UV or chlorine and by combined applications of UV and chlorine in either a sequential or simultaneous manner. The treated samples were then propagated in human lung carcinoma epithelial cells to quantify the log inactivation of HAdV-5. When the processes were separate, a fluence of 100 mJ/cm(2) and a CT value of 0.02 mg min/L were required to achieve 2 log inactivation of HAdV-5 by UV disinfection and chlorination, respectively. Interestingly, synergistic effects on the HAdV-5 inactivation rates were found in the sequential process of chlorine followed by UV (Cl2-UV) (p simultaneous application of UV/Cl2. This implies that a pretreatment with chlorine may increase the sensitivity of the virus to the subsequent UV disinfection. In conclusion, this study suggests that the combined application of UV and chlorine could be an effective measure against adenoviruses as a multi-barrier approach in water disinfection.

  3. Coating of adenovirus type 5 with polymers containing quaternary amines prevents binding to blood components

    Czech Academy of Sciences Publication Activity Database

    Šubr, Vladimír; Kostka, Libor; Selby-Milic, T.; Fisher, K.; Ulbrich, Karel; Seymour, W.; Carlisle, R. C.

    2009-01-01

    Roč. 135, č. 2 (2009), s. 152-158 ISSN 0168-3659 R&D Projects: GA AV ČR KJB400500803 EU Projects: European Commission(XE) 512087 - GIANT Institutional research plan: CEZ:AV0Z40500505 Keywords : quaternary ammonium * HPMA * adenovirus Subject RIV: CD - Macromolecular Chemistry Impact factor: 5.949, year: 2009

  4. Studies on the mechanism of replication of adenovirus DNA. IV. Discontinuous DNA chain propagation

    NARCIS (Netherlands)

    Vlak, J.M.; Rozijn, Th.H.; Sussenbach, J.S.

    The replication of adenovirus type 5 DNA occurs by discontinuous chain propagation via short pieces of DNA. These pieces accumulate if the infected cells are treated with hydroxyurea. They have a sedimentation coefficient of 11 S corresponding to a molecular weight of about 700,000, and they contain

  5. Fowl adenovirus serotype 9 vectored vaccine for protection of avian influenza virus

    Science.gov (United States)

    A fowl adenovirus serotype 9, a non-pathogenic large double stranded DNA virus, was developed as a viral vector to express influenza genes as a potential vaccine. Two separate constructs were developed that expressed either the hemagglutinin gene of A/Chicken/Jalisco/2012 (H7) or A/ Chicken/Iowa/20...

  6. Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater

    Science.gov (United States)

    Enteroviruses, noroviruses and adenoviruses are among the most common viruses infecting humans worldwide. These viruses are shed in the feces of infected individuals and can accumulate in wastewater. Therefore, wastewater is a source of a potentially diverse group of enteric viru...

  7. Oncogenicity by adenovirus is not determined by the transforming region only

    NARCIS (Netherlands)

    Bernards, R.A.; Leeuw, M.G.W. de; Vaessen, M.J.; Houweling, A.; Eb, A.J. van der

    1984-01-01

    We have constructed a nondefective recombinant virus between the nononcogenic adenovirus 5 (Ad5) and the highly oncogenic Ad12. The recombinant genome consists essentially of Ad5 sequences, with the exception of the transforming early region 1 (E1) which is derived from Ad12. HeLa cells infected

  8. A double-regulated oncolytic adenovirus with improved safety for adenocarcinoma therapy

    International Nuclear Information System (INIS)

    Wei, Na; Fan, Jun Kai; Gu, Jin Fa; He, Ling Feng; Tang, Wen Hao; Cao, Xin; Liu, Xin Yuan

    2009-01-01

    Safety and efficiency are equally important to be considered in developing oncolytic adenovirus. Previously, we have reported that ZD55, an oncolytic adenovirus with the deletion of E1B-55K gene, exhibited potent antitumor activity. In this study, to improve the safety of ZD55, we utilized MUC1 promoter to replace the native promoter of E1A on the basis of ZD55, and generated a double-regulated adenovirus, named MUD55. Our data demonstrated that the expression of early and late genes of MUD55 was both reduced in MUC1-negative cells, resulting in its stricter glandular-tumor selective progeny production. The cytopathic effect of MUD55 was about 10-fold lower than mono-regulated adenovirus ZD55 or Ad.MUC1 in normal cells and not obviously attenuated in glandular tumor cells. Moreover, MUD55 showed the least liver toxicity when administrated by intravenous injection in nude mice. These results indicate that MUD55 could be a promising candidate for the treatment of adenocarcinoma.

  9. Effect of organic carbon on sorption of human adenovirus to soil particles and laboratory containers

    Science.gov (United States)

    A key factor controlling the relationship between virus release and human exposure is how virus particles interact with soils, sediments and other solid particles in the environment and in engineered treatment systems. Finding no previous investigations of human adenovirus (HAdV)...

  10. Detection of adenovirus hexon sequence in a cat by polymerase chain reaction(short communication)

    NARCIS (Netherlands)

    Horzinek, M.C.; Lakatos, B.; Farkas, J.; Egberink, H.F.; Vennema, H.; Benko, M.

    1999-01-01

    Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in pharyngeal and rectal swab samples of a cat seropositive for adenovirus and suffering from transient hepatic failure. The samples were taken at a one-year interval, and both faecal samples as well as the second pharyngeal

  11. uPAR-controlled oncolytic adenoviruses eliminate cancer stem cells in human pancreatic tumors.

    Science.gov (United States)

    Sobrevals, Luciano; Mato-Berciano, Ana; Urtasun, Nerea; Mazo, Adela; Fillat, Cristina

    2014-01-01

    Pancreatic tumors contain cancer stem cells highly resistant to chemotherapy. The identification of therapies that can eliminate this population of cells might provide with more effective treatments. In the current work we evaluated the potential of oncolytic adenoviruses to act against pancreatic cancer stem cells (PCSC). PCSC from two patient-derived xenograft models were isolated from orthotopic pancreatic tumors treated with saline, or with the chemotherapeutic agent gemcitabine. An enrichment in the number of PCSC expressing the cell surface marker CD133 and a marked enhancement on tumorsphere formation was observed in gemcitabine treated tumors. No significant increase in the CD44, CD24, and epithelial-specific antigen (ESA) positive cells was observed. Neoplastic sphere-forming cells were susceptible to adenoviral infection and exposure to oncolytic adenoviruses resulted in elevated cytotoxicity with both Adwt and the tumor specific AduPARE1A adenovirus. In vivo, intravenous administration of a single dose of AduPARE1A in human-derived pancreatic xenografts led to a remarkable anti-tumor effect. In contrast to gemcitabine AduPARE1A treatment did not result in PCSC enrichment. No enrichment on tumorspheres neither on the CD133(+) population was detected. Therefore our data provide evidences of the relevance of uPAR-controlled oncolytic adenoviruses for the elimination of pancreatic cancer stem cells. © 2013.

  12. Large-scale adenovirus and poxvirus-vectored vaccine manufacturing to enable clinical trials.

    Science.gov (United States)

    Kallel, Héla; Kamen, Amine A

    2015-05-01

    Efforts to make vaccines against infectious diseases and immunotherapies for cancer have evolved to utilize a variety of heterologous expression systems such as viral vectors. These vectors are often attenuated or engineered to safely deliver genes encoding antigens of different pathogens. Adenovirus and poxvirus vectors are among the viral vectors that are most frequently used to develop prophylactic vaccines against infectious diseases as well as therapeutic cancer vaccines. This mini-review describes the trends and processes in large-scale production of adenovirus and poxvirus vectors to meet the needs of clinical applications. We briefly describe the general principles for the production and purification of adenovirus and poxvirus viral vectors. Currently, adenovirus and poxvirus vector manufacturing methods rely on well-established cell culture technologies. Several improvements have been evaluated to increase the yield and to reduce the overall manufacturing cost, such as cultivation at high cell densities and continuous downstream processing. Additionally, advancements in vector characterization will greatly facilitate the development of novel vectored vaccine candidates. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Fatal Fulminant Hepatic Failure from Adenovirus in Allogeneic Bone Marrow Transplant Patients

    Directory of Open Access Journals (Sweden)

    Jatin M. Vyas

    2012-01-01

    Full Text Available We report two cases of fatal hepatic failure in patients who received matched unrelated bone marrow transplantation. Both patients presented with high fevers, abnormal liver functions tests, and hypodense lesions in the liver by CT scan. Histologic examination of postmortem liver samples demonstrated extensive necrosis, and immunohistochemistry was positive for adenovirus.

  14. Multiple cross-species transmission events of human adenoviruses (HAdV) during hominine evolution

    Czech Academy of Sciences Publication Activity Database

    Hoppe, E.; Pauly, M.; Gillespie, T. R.; Akoua-Koffi, C.; Hohmann, G.; Fruth, B.; Karhemere, S.; Madinda, N. F.; Mugisha, L.; Muyembe, J.-J.; Todd, A.; Petrželková, Klára Judita; Gray, M.; Robbins, M.; Bergl, R. A.; Wittig, R. M.; Zuberbuehler, K.; Boesch, C.; Schubert, G.; Leendertz, F. H.; Ehlers, B.; Calvignac-Spencer, S.

    2015-01-01

    Roč. 32, č. 8 (2015), s. 2072-2084 ISSN 0737-4038 R&D Projects: GA ČR GA206/09/0927 Institutional support: RVO:68081766 Keywords : adenovirus * African great apes * zoonosis Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 13.649, year: 2015

  15. Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins

    Czech Academy of Sciences Publication Activity Database

    Bachtarzi, H.; Stevenson, M.; Šubr, Vladimír; Ulbrich, Karel; Seymour, L. W.; Fisher, K. D.

    2011-01-01

    Roč. 150, č. 2 (2011), s. 196-203 ISSN 0168-3659 R&D Projects: GA MŠk 1M0505 Institutional research plan: CEZ:AV0Z40500505 Keywords : E-selectin * pHPMA * adenovirus Subject RIV: EI - Biotechnology ; Bionics Impact factor: 5.732, year: 2011

  16. Production and purification of non replicative canine adenovirus type 2 derived vectors.

    Science.gov (United States)

    Szelechowski, Marion; Bergeron, Corinne; Gonzalez-Dunia, Daniel; Klonjkowski, Bernard

    2013-12-03

    Adenovirus (Ad) derived vectors have been widely used for short or long-term gene transfer, both for gene therapy and vaccine applications. Because of the frequent pre-existing immunity against the classically used human adenovirus type 5, canine adenovirus type 2 (CAV2) has been proposed as an alternative vector for human gene transfer. The well-characterized biology of CAV2, together with its ease of genetic manipulation, offer major advantages, notably for gene transfer into the central nervous system, or for inducing a wide range of protective immune responses, from humoral to cellular immunity. Nowadays, CAV2 represents one of the most appealing nonhuman adenovirus for use as a vaccine vector. This protocol describes a simple method to construct, produce and titer recombinant CAV2 vectors. After cloning the expression cassette of the gene of interest into a shuttle plasmid, the recombinant genomic plasmid is obtained by homologous recombination in the E. coli BJ5183 bacterial strain. The resulting genomic plasmid is then transfected into canine kidney cells expressing the complementing CAV2-E1 genes (DK-E1). A viral amplification enables the production of a large viral stock, which is purified by ultracentrifugation through cesium chloride gradients and desalted by dialysis. The resulting viral suspension routinely has a titer of over 10(10) infectious particles per ml and can be directly administrated in vivo.

  17. Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit

    NARCIS (Netherlands)

    Agostino, D' C.; Cook, N.; Bartolo, Di I.; Ruggeri, F.M.; Berto, A.; Martelli, F.; Banks, M.; Vasickova, P.; Kralik, P.; Pavlik, I.; Kokkinos, P.; Vantarakis, A.; Söderberg, K.; Maunula, L.; Verhaelen, K.; Rutjes, S.; Roda Husman, De A.M.; Hakze-van der Honing, van der R.W.; Poel, van der W.H.M.; Kaupke, A.; Kozyra, I.; Rzezutka, A.; Prodanov, J.; Lazic, S.; Petrovic, T.; Carratala, A.; Gironés, R.; Diez-Valcarce, M.; Hernandez, M.; Rodriguez-Lazaro, D.

    2012-01-01

    The qualitative performance characteristics of a qPCR-based method to detect human adenoviruses in raspberries were determined through a collaborative trial involving 11 European laboratories. The method incorporated a sample process control (murine norovirus) and an internal amplification control.

  18. Molecular epidemiology of human adenovirus infections in Denmark, 2011–2016

    DEFF Research Database (Denmark)

    Barnadas, Céline; Schmidt, Dennis Jelsbak; Fischer, Thea K.

    2018-01-01

    Background: Human adenoviruses (HAdVs) can cause respiratory tract infections, conjunctivitis, diarrhoea and outbreaks have been reported. However, little is known about the disease burden and the molecular epidemiology of HAdV. Objectives: To retrospectively perform a molecular characterization ...

  19. Adenovirus 36 DNA in Adipose Tissue of Patient with Unusual Visceral Obesity

    Science.gov (United States)

    Salehian, Behrouz; Forman, Stephen J.; Kandeel, Fouad R.; Bruner, Denise E.; He, Jia

    2010-01-01

    Massive adipose tissue depositions in the abdomen and thorax sufficient to interfere with respiration developed in a patient with multiple medical problems. Biopsy of adipose tissue identified human adenovirus 36 (Adv 36) DNA. Adv 36 causes adipogenesis in animals and humans. Development of massive lipomatosis may be caused by Adv 36. PMID:20409382

  20. Epidermal growth factor receptor targeting of replication competent adenovirus enhances cytotoxicity in bladder cancer

    NARCIS (Netherlands)

    van der Poel, HG; Molenaar, B; van Beusechem, VW; Haisma, HJ; Rodriguez, R; Curiel, DT; Gerritsen, WR

    Purpose: We evaluated the delivery and oncolytic potential of targeted replication competent adenoviruses in bladder cancer lines. Materials and Methods: Seven established human bladder cancer tumor lines (5637, SW800, TCCsup, J82, Scaber, T24 and 253J) were studied for the expression of integrins

  1. Fatal adenovirus encephalomyeloradiculitis in an umbilical cord stem cell transplant recipient

    OpenAIRE

    Awosika, Oluwole O.; Lyons, Jennifer L.; Ciarlini, Pedro; Phillips, Richard E.; Alfson, Elizabeth D.; Johnson, Emily L.; Koo, Sophia; Marty, Francisco; Drew, Clifton; Zaki, Sherif; Folkerth, Rebecca D.; Klein, Joshua P.

    2013-01-01

    Adenovirus infections frequently complicate allogeneic stem cell transplants but nervous system involvement, usually presenting as encephalitis, is atypical. Progression from encephalitis to myeloradiculitis has not been described previously.1 We present a unique case of fatal adenoviral encephalomyeloradiculitis with imaging and pathologic correlates.

  2. Role of the adenovirus early region 1B tumor antigens in transformation and lytic infection

    NARCIS (Netherlands)

    Bernards, R.A.; Leeuw, M.G.W. de; Houweling, A.; Eb, A.J. van der

    1986-01-01

    We have investigated the contribution of each of the two adenovirus type 5 (Ad5) major early region lb (Elb) proteins in cell transformation and in lytic infection. An Ad5 El plasmid, in which the reading frame for the 19-kDa Elb protein was abolished by a stop codon close to the initiation codon,

  3. Adenovirus DNA replication in vitro is stimulated by RNA from uninfected HeLa cells

    NARCIS (Netherlands)

    Vliet, P.C. van der; Dam, D. van; Kwant, M.M.

    1984-01-01

    Adenovirus DNA replication was studied in a partially reconstituted system consisting of purified viral proteins (DNA-binding protein, precursor terminal protein and Ad DNA polymerase) and a nuclear extract from uninfected HeLa cells. Optimal DNA replication required the presence of a heat-stable,

  4. Transcriptional activation by the E1A regions of adenovirus types 40 and 41

    NARCIS (Netherlands)

    Loon, A.E. van; Gilardi, P.; Perricaudet, M.; Rozijn, Th. H.; Sussenbach, J.S.

    In order to establish whether the poor growth of the two fastidious adenoviruses types 40 and 41 (Ad40 and Ad41) in HeLa cells is due to a reduced trans-activation by the early region to (E1A), we have determined the trans-activating effect of this region on the expression of the chloramphenicol

  5. Innovation through Joint Ventures. Implications and Role of the Top Management Team Innovación a través de Joint Ventures. Implicaciones y papel del Equipo de Alta Dirección

    Directory of Open Access Journals (Sweden)

    Andrea Ruiz Restrepo,

    2009-06-01

    directiva con relación a las empresas madres pueden dificultar la consecución de los objetivos para los que fueron creadas. También se consideran otros factores que pueden ayudar a corregir las implicaciones negativas sobre la innovación, generadas por la composición de los equipos y las características de su labor.  

  6. Human Adenovirus 36 Infection Increased the Risk of Obesity

    Science.gov (United States)

    Xu, Mei-Yan; Cao, Bing; Wang, Dong-Fang; Guo, Jing-Hui; Chen, Kai-Li; Shi, Mai; Yin, Jian; Lu, Qing-Bin

    2015-01-01

    Abstract Human adenovirus 36 (HAdV-36), as the key pathogen, was supposed and discussed to be associated with obesity. We searched the references on the association between HAdV-36 infection and obesity with the different epidemiological methods, to explore the relationship with a larger sample size by meta-analysis and compare the differences of epidemiological methods and population subsets by the subgroup analyses. We conducted literature search on the association between HAdV-36 infections and obesity in English or Chinese published up to July 1, 2015. The primary outcome was the HAdV-36 infection rate in the obese and lean groups; the secondary outcomes were the BMI level and BMI z-score in the HAdV-36 positive and negative groups. The pooled odds ratio (OR) was calculated for the primary outcome; the standardized mean differences (SMDs) were calculated for the secondary and third outcomes. Prediction interval (PI) was graphically presented in the forest plot of the random effect meta-analyses. Metaregression analysis and subgroup analysis were performed. Finally 24 references with 10,191 study subjects were included in the meta-analysis. The obesity subjects were more likely to be infected with HAdV-36 compared to the lean controls (OR = 2.00; 95%CI: 1.46, 2.74; PI: 0.59, 6.76; P infection for obesity were 1.77 (95%CI: 1.19, 2.63; PI: 0.44, 7.03; P = 0.005) and 2.26 (95%CI: 1.67, 3.07; PI: 1.45, 3.54; P SMD of BMI was 0.28 (95% CI: 0.08, 0.47; PI: −0.53, 1.08; P = 0.006) in the HAdV-36 positive subjects with a high heterogeneity (I2 = 86.5%; P infection was higher than those without HAdV-36 infection (SMD = 0.19; 95%CI: −0.31, 0.70; PI: −2.10, 2.49), which had no significantly statistical difference (P = 0.453). HAdV-36 infection increased the risk of obesity. HAdV-36 also increased the risk of weight gain in adults, which was not observed in children. PMID:26705235

  7. [Effect of topical application of a recombinant adenovirus carrying promyelocytic leukemia gene in a psoriasis-like mouse model].

    Science.gov (United States)

    Wang, Qiongyu; Zhang, Aijun; Ma, Huiqun; Wang, Shijie; Ma, Yunyun; Zou, Xingwei; Li, Ruilian

    2013-03-01

    To investigate the effects of topical treatment with adenovirus-mediated promyelocytic leukemia gene (PML) gene in a psoriasis-like mouse model. The effect of adenovirus-mediated PML gene on the granular layer of mouse tail scale epidermis and epithelial mitosis were observed on longitudinal histological sections prepared from the tail skin and vaginal epithelium of the mice. Adenovirus-mediated PML gene significantly inhibited mitosis of mouse vaginal epithelial cells and promoted the formation of granular layer in mouse tail scale epidermis. The therapeutic effect of PML gene in the psoriasis-like mouse model may be associated with increased granular cells and suppressed epidemic cell proliferation.

  8. Conceptos generales sobre ABA en niños con trastorno del espectro autista

    Directory of Open Access Journals (Sweden)

    Sandra Elizabeth Piñeros Ortiz

    2012-01-01

    Full Text Available Los trastornos del espectro autista (TEA son entidades que generan discapacidad. Diversas intervenciones psicofarmacológicas y psicosociales modulan algunas de las alteraciones comportamentales asociadas y mejoran la calidad de vida de las personas afectadas y de sus cuidadores. La terapia ABA es una de las intervenciones psicosociales más conocidas y utilizada en población con TEA. Mediante de la  formulación de preguntas clave y sus respuestas, este artículo realiza una breve descripción de los aspectos históricos, las principales características y los fundamentos teóricos del ABA. Se discuten los resultados de diversos estudios que señalan las limitaciones metodológicas de las investigaciones sobre  la efectividad de esta terapia y sus implicaciones para la práctica clínica.

  9. COSTO ENERGÉTICO DE PROCESOS CEREBRALES CON ESPECIAL ÉNFASIS EN APRENDIZAJE Y MEMORIA

    Directory of Open Access Journals (Sweden)

    L. R. Lareo

    2006-12-01

    Full Text Available En el presenta trabajo se reportan algunos cálculos básicos sobre la utilización de energía en el cerebro, para su funcionamiento normal, con algunas predicciones sobre la parte de esta energía que es empleada en los procesos de aprendizaje y memoria. Las implicaciones de la actual imposibilidad de transferencia directa de conocimiento de los padres a su progenie y los costos económicos de esta “ineficiencia” hacen que este problema sea propuesto como una de las fronteras de la investigación en las neurociencias, en particular las neurociencias computacionales, y la neuroinformática. Se culmina asumiendo como un reto investigativo la propuesta del futurólogo inglés Ian Pearson sobre la cercana posibilidad de interacción directa computador-cerebro.

  10. Neurología de la anticipación y sus implicaciones en el deporte

    Directory of Open Access Journals (Sweden)

    Juan Felipe Correa Mesa

    2016-01-01

    Por otro lado, se aborda la integración de centros puntuales del sistema nervioso central y redes neuronales que permiten el entramado de procesos de aprendizaje por observación, además de proveer equilibrio y eficiencia al sistema en la recepción de estímulos y su relación con la generación de eferencias motoras que cumplan con objetivos específicos. En el ámbito deportivo estos procesos favorecen la eficiencia del gesto optimizando el movimiento.

  11. TLC Colombia-Estados Unidos y sus implicaciones en el turismo

    Directory of Open Access Journals (Sweden)

    Nancy Rocío Rueda Esteban

    2012-11-01

    Full Text Available El TLC entre Colombia y Estados Unidos presenta tanto ventajas como desventajas que hay que entender para poder afrontar. Con el presente artículo se pretende analizar y aclarar la importancia de los cambios que puede llegar a generar el TLC Colombia-Estados Unidos sobre el sector turístico y hotelero de Colombia. Se expone una idea clara sobre las necesidades del país para tener una oportunidad amplia y fuerte sobre los cambios económicos, sociales y culturales que sobrevienen con la firma y entrada en vigencia del tratado.

  12. Implicaciones sedimentológicas sobre el cambio en la cobertura del bosque de manglar en Boca Zacate, Humedal Nacional Térraba-Sierpe, Costa Rica

    Directory of Open Access Journals (Sweden)

    Ana Margarita Silva Benavides

    2015-09-01

    Full Text Available Resumen:En los últimos sesenta años han ocurrido cambios en el Humedal Nacional Térraba-Sierpe, Costa Rica. Los cambios en la geomorfología costera están asociados a la erosión o acreción de sedimentos, lo que ha favorecido la eliminación de sectores de bosque o a la formación de bancos de sedimentos que han sido colonizados por árboles de mangle. El estudio se realizó en los sectores Bocón, Brujo e isla Coco en Boca Zacate, Humedal Nacional Térraba-Sierpe, Costa Rica, entre 2008-2013, cuyo objetivo consiste en analizar el aporte de sedimentos y las implicaciones en la cobertura del bosque de mangle en el sector de Boca Zacate. Es este estudio se realizó un análisis multitemporal de la morfología litoral con fotografías aéreas e imágenes satelitales de 1948, 1960, 1974, 1978, 1984, 1992 y 2011. Y además, se cuantificó la tasa de sedimentación mensual (g/cm2/día en los canales de los estuarios, la composición granulométrica y la concentración de elementos químicos en los sedimentos (mgL-1 en época seca y lluviosa durante los años de estudio. Los resultados indican que Boca Zacate ha mantenido en los últimos 60 años una tendencia hacia la erosión, perdiendo un 10.6 % de su espacio emergido entre 1948 y 2011 y aproximadamente un 8.9 % de su cobertura forestal; también ha experimentado una acreción en la zona de la isla Coco, uniéndose al continente por este sector. La tasa de sedimentación cuantificada en los esteros presentó diferencias con respecto al sitio y a la época. La mayor cantidad se presentó en el estero el Brujo y la menor cantidad en el estero Camíbar. Con respecto a la granulo-metría, el tipo de sedimento dominante es la arena, seguido por la arcilla y en menor cantidad el limo en todos los sitios de estudio. La mayor distribución de los elementos químicos corresponde al magnesio, calcio, potasio; mientras que el manganeso, hierro, aluminio, fósforo, zinc y cobre se encontraron en menor

  13. LA PSICOLOGIZACIÓN DE LA EDUCACIÓN: IMPLICACIONES PEDAGÓGICAS DE LA INTELIGENCIA EMOCIONAL Y LA PSICOLOGÍA POSITIVA

    Directory of Open Access Journals (Sweden)

    Miriam Prieto Egido

    2018-01-01

    Full Text Available En la actualidad, teorías provenientes del campo de la Psicología, especial- mente la Inteligencia Emocional y la Psicología Positiva, lideran los discursos y prácticas educativos. Sin embargo, dicho liderazgo ha sido objeto de esca- sos análisis desde el ámbito de conocimiento de la Teoría de la Educación, responsable, por su carácter normativo, de re ̄exionar acerca de los ®nes de la educación. Tomando como referencia los estudios provenientes de la Teoría de la Educación británica, el presente artículo aborda el análisis de las implicaciones de las teorías psicológicas para la educación. Para ello, en primer lugar se realiza una descripción de las bases de la Inteligencia Emo- cional y la Psicología Positiva, así como se muestran algunos ejemplos de su traducción educativa. En la segunda parte del artículo se aborda el análisis pedagógico, que se conceptualiza en términos de reducción en dos aspectos esenciales de la educación: el sujeto de la educación y la relación educativa. Se concluye que la reducción del sujeto de la educación es consecuencia de una concepción disminuida tanto de las posibilidades del sujeto, como de la experiencia emocional y de la propia racionalidad. Por su parte, la reducción de la relación educativa se produce a través de su conversión en una relación comunicativa, en la que el discurso sustituye a la acción. Se concluye que la educación tiene un carácter propio, diferente al de la psicología. Si bien la educación debe tomar en consideración el conocimiento aportado por la psicología, debe también mantener su carácter propio, basado en la con®anza de las posibilidades de los sujetos que se educan como principio y en la bús- queda del bien en su relación con el mundo y con los otros como ®nalidad. Así mismo, la relación educativa debe estar basada en la intencionalidad del educador y en la acción en lugar del discurso.

  14. Identification of a novel aviadenovirus, designated pigeon adenovirus 2 in domestic pigeons (Columba livia).

    Science.gov (United States)

    Teske, L; Rubbenstroth, D; Meixner, M; Liere, K; Bartels, H; Rautenschlein, S

    2017-01-02

    The young pigeon disease syndrome (YPDS) affects mainly young pigeons of less than one year of age and leads to crop stasis, vomitus, diarrhea, anorexia and occasionally death. This disease is internationally a major health problem because of its seasonal appearance during competitions such as homing pigeon races or exhibitions of ornamental birds. While the etiology of YPDS is still unclear, adenoviruses are frequently discussed as potential causative agents. Electron microscopy of feces from a YPDS outbreak revealed massive shedding of adenovirus-like particles. Whole genome sequencing of this sample identified a novel adenovirus tentatively named pigeon adenovirus 2 (PiAdV-2). Phylogenetic and comparative genome analysis suggest PiAdV-2 to belong to a new species within the genus Aviadenovirus, for which we propose the name Pigeon aviadenovirus B. The PiAdV-2 genome shares 54.9% nucleotide sequence identity with pigeon adenovirus 1 (PiAdV-1). In a screening of further YPDS-affected flocks two variants of PiAdV-2 (variant A and B) were detected which shared 97.6% nucleotide identity of partial polymerase sequences, but only 79.7% nucleotide identity of partial hexon sequences. The distribution of both PiAdV-2 variants was further investigated in fecal samples collected between 2008 and 2015 from healthy or YPDS-affected racing pigeons of different lofts. Independent of their health status, approximately 20% of young and 13% of adult pigeon flocks harbored PiAdV-2 variants. Birds were free of PiAdV-1 or other aviadenoviruses as determined by PCRs targeting the aviadenovirus polymerase or the PiAdV-1 fiber gene, respectively. In conclusion, there is no indication of a correlation between YPDS outbreaks and the presence of PiAdV-2 or other aviadenoviruses, arguing against an causative role in this disease complex. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. Targeting human breast cancer cells by an oncolytic adenovirus using microRNA-targeting strategy.

    Science.gov (United States)

    Shayestehpour, Mohammad; Moghim, Sharareh; Salimi, Vahid; Jalilvand, Somayeh; Yavarian, Jila; Romani, Bizhan; Mokhtari-Azad, Talat

    2017-08-15

    MicroRNA-targeting strategy is a promising approach that enables oncolytic viruses to replicate in tumor cells but not in normal cells. In this study, we targeted adenoviral replication toward breast cancer cells by inserting ten complementary binding sites for miR-145-5p downstream of E1A gene. In addition, we evaluated the effect of increasing miR-145 binding sites on inhibition of virus replication. Ad5-control and adenoviruses carrying five or ten copies of miR145-5p target sites (Ad5-5miR145T, Ad5-10miR145T) were generated and inoculated into MDA-MB-453, BT-20, MCF-7 breast cancer cell lines and human mammary epithelial cells (HMEpC). Titer of Ad5-10miR145T in HMEpC was significantly lower than Ad5-control titer. Difference between the titer of these two viruses at 12, 24, 36, and 48h after infection was 1.25, 2.96, 3.06, and 3.77 log TCID 50 . No significant difference was observed between the titer of both adenoviruses in MDA-MB-453, BT-20 and MCF-7 cells. The infectious titer of adenovirus containing 10 miR-145 binding sites in HMEpC cells at 24, 36, and 48h post-infection was 1.7, 2.08, and 4-fold, respectively, lower than the titer of adenovirus carrying 5 miR-145 targets. Our results suggest that miR-145-targeting strategy provides selectivity for adenovirus replication in breast cancer cells. Increasing the number of miRNA binding sites within the adenoviral genome confers more selectivity for viral replication in cancer cells. Copyright © 2017. Published by Elsevier B.V.

  16. Experimental cross-species infection of common marmosets by titi monkey adenovirus.

    Directory of Open Access Journals (Sweden)

    Guixia Yu

    Full Text Available Adenoviruses are DNA viruses that infect a number of vertebrate hosts and are associated with both sporadic and epidemic disease in humans. We previously identified a novel adenovirus, titi monkey adenovirus (TMAdV, as the cause of a fulminant pneumonia outbreak in a colony of titi monkeys (Callicebus cupreus at a national primate center in 2009. Serological evidence of infection by TMAdV was also found in a human researcher at the facility and household family member, raising concerns for potential cross-species transmission of the virus. Here we present experimental evidence of cross-species TMAdV infection in common marmosets (Callithrix jacchus. Nasal inoculation of a cell cultured-adapted TMAdV strain into three marmosets produced an acute, mild respiratory illness characterized by low-grade fever, reduced activity, anorexia, and sneezing. An increase in virus-specific neutralization antibody titers accompanied the development of clinical signs. Although serially collected nasal swabs were positive for TMAdV for at least 8 days, all 3 infected marmosets spontaneously recovered by day 12 post-inoculation, and persistence of the virus in tissues could not be established. Thus, the pathogenesis of experimental inoculation of TMAdV in common marmosets resembled the mild, self-limiting respiratory infection typically seen in immunocompetent human hosts rather than the rapidly progressive, fatal pneumonia observed in 19 of 23 titi monkeys during the prior 2009 outbreak. These findings further establish the potential for adenovirus cross-species transmission and provide the basis for development of a monkey model useful for assessing the zoonotic potential of adenoviruses.

  17. The Revolution in Viral Genomics as Exemplified by the Bioinformatic Analysis of Human Adenoviruses

    Directory of Open Access Journals (Sweden)

    Sarah Torres

    2010-06-01

    Full Text Available Over the past 30 years, genomic and bioinformatic analysis of human adenoviruses has been achieved using a variety of DNA sequencing methods; initially with the use of restriction enzymes and more currently with the use of the GS FLX pyrosequencing technology. Following the conception of DNA sequencing in the 1970s, analysis of adenoviruses has evolved from 100 base pair mRNA fragments to entire genomes. Comparative genomics of adenoviruses made its debut in 1984 when nucleotides and amino acids of coding sequences within the hexon genes of two human adenoviruses (HAdV, HAdV–C2 and HAdV–C5, were compared and analyzed. It was determined that there were three different zones (1-393, 394-1410, 1411-2910 within the hexon gene, of which HAdV–C2 and HAdV–C5 shared zones 1 and 3 with 95% and 89.5% nucleotide identity, respectively. In 1992, HAdV-C5 became the first adenovirus genome to be fully sequenced using the Sanger method. Over the next seven years, whole genome analysis and characterization was completed using bioinformatic tools such as blastn, tblastx, ClustalV and FASTA, in order to determine key proteins in species HAdV-A through HAdV-F. The bioinformatic revolution was initiated with the introduction of a novel species, HAdV-G, that was typed and named by the use of whole genome sequencing and phylogenetics as opposed to traditional serology. HAdV bioinformatics will continue to advance as the latest sequencing technology enables scientists to add to and expand the resource databases. As a result of these advancements, how novel HAdVs are typed has changed. Bioinformatic analysis has become the revolutionary tool that has significantly accelerated the in-depth study of HAdV microevolution through comparative genomics.

  18. A dynamical systems model for combinatorial cancer therapy enhances oncolytic adenovirus efficacy by MEK-inhibition.

    Science.gov (United States)

    Bagheri, Neda; Shiina, Marisa; Lauffenburger, Douglas A; Korn, W Michael

    2011-02-01

    Oncolytic adenoviruses, such as ONYX-015, have been tested in clinical trials for currently untreatable tumors, but have yet to demonstrate adequate therapeutic efficacy. The extent to which viruses infect targeted cells determines the efficacy of this approach but many tumors down-regulate the Coxsackievirus and Adenovirus Receptor (CAR), rendering them less susceptible to infection. Disrupting MAPK pathway signaling by pharmacological inhibition of MEK up-regulates CAR expression, offering possible enhanced adenovirus infection. MEK inhibition, however, interferes with adenovirus replication due to resulting G1-phase cell cycle arrest. Therefore, enhanced efficacy will depend on treatment protocols that productively balance these competing effects. Predictive understanding of how to attain and enhance therapeutic efficacy of combinatorial treatment is difficult since the effects of MEK inhibitors, in conjunction with adenovirus/cell interactions, are complex nonlinear dynamic processes. We investigated combinatorial treatment strategies using a mathematical model that predicts the impact of MEK inhibition on tumor cell proliferation, ONYX-015 infection, and oncolysis. Specifically, we fit a nonlinear differential equation system to dedicated experimental data and analyzed the resulting simulations for favorable treatment strategies. Simulations predicted enhanced combinatorial therapy when both treatments were applied simultaneously; we successfully validated these predictions in an ensuing explicit test study. Further analysis revealed that a CAR-independent mechanism may be responsible for amplified virus production and cell death. We conclude that integrated computational and experimental analysis of combinatorial therapy provides a useful means to identify treatment/infection protocols that yield clinically significant oncolysis. Enhanced oncolytic therapy has the potential to dramatically improve non-surgical cancer treatment, especially in locally advanced

  19. The complete nucleotide sequence, genome organization, and origin of human adenovirus type 11

    International Nuclear Information System (INIS)

    Stone, Daniel; Furthmann, Anne; Sandig, Volker; Lieber, Andre

    2003-01-01

    The complete DNA sequence and transcription map of human adenovirus type 11 are reported here. This is the first published sequence for a subgenera B human adenovirus and demonstrates a genome organization highly similar to those of other human adenoviruses. All of the genes from the early, intermediate, and late regions are present in the expected locations of the genome for a human adenovirus. The genome size is 34,794 bp in length and has a GC content of 48.9%. Sequence alignment with genomes of groups A (Ad12), C (Ad5), D (Ad17), E (Simian adenovirus 25), and F (Ad40) revealed homologies of 64, 54, 68, 75, and 52%, respectively. Detailed genomic analysis demonstrated that Ads 11 and 35 are highly conserved in all areas except the hexon hypervariable regions and fiber. Similarly, comparison of Ad11 with subgroup E SAV25 revealed poor homology between fibers but high homology in proteins encoded by all other areas of the genome. We propose an evolutionary model in which functional viruses can be reconstituted following fiber substitution from one serotype to another. According to this model either the Ad11 genome is a derivative of Ad35, from which the fiber was substituted with Ad7, or the Ad35 genome is the product of a fiber substitution from Ad21 into the Ad11 genome. This model also provides a possible explanation for the origin of group E Ads, which are evolutionarily derived from a group C fiber substitution into a group B genome

  20. Thin-section computed tomography findings in 104 immunocompetent patients with adenovirus pneumonia.

    Science.gov (United States)

    Park, Chan Kue; Kwon, Hoon; Park, Ji Young

    2017-08-01

    Background To date, there has been no computed tomography (CT) evaluation of adenovirus pneumonia in a large number of immunocompetent patients. Purpose To describe the thin-section CT findings of immunocompetent patients with adenovirus pneumonia. Material and Methods We prospectively enrolled 104 patients with adenovirus pneumonia from a military hospital. CT scans of each patient were retrospectively and independently assessed by two radiologists for the presence of abnormalities, laterality and zonal predominance of the parenchymal abnormalities, and dominant imaging patterns and their anatomic distributions. Results CT findings included consolidation (n = 92), ground-glass opacity (GGO; n = 82), septal thickening (n = 34), nodules (n = 46), bronchial wall thickening (n = 32), pleural effusion (n = 16), and lymphadenopathy (n = 3). Eighty-four patients (81%) exhibited unilateral parenchymal abnormalities and 57 (57%) exhibited lower lung zone abnormalities. The most frequently dominant CT pattern was consolidation with surrounding GGO (n = 50), with subpleural (70%) and peribronchovascular (94%) distributions. Consolidation-the second-most common pattern (n = 33)-also exhibited subpleural (79%) and peribronchovascular (97%) distributions. The dominant nodule pattern (n = 14) exhibited mixed (64%) and peribronchovascular (100%) distributions. A dominant GGO pattern was only observed in four patients; none had central distribution. Conclusion Although the manifestations of adenovirus pneumonia on CT are varied, we found the most frequent pattern was consolidation with or without surrounding GGO, with subpleural and peribronchovascular distributions. Parenchymal abnormalities were predominantly unilateral and located in the lower lung zone. If dominant consolidation findings are present in immunocompetent patients during the early stages, adenovirus pneumonia should be considered.

  1. Diagnosis of eight groups of xeroderma pigmentosum by genetic complementation using recombinant adenovirus vectors.

    Science.gov (United States)

    Yamashita, Toshiharu; Okura, Masae; Ishii-Osai, Yasue; Hida, Tokimasa

    2016-10-01

    Because patients with xeroderma pigmentosum (XP) must avoid ultraviolet (UV) light from an early age, an early diagnosis of this disorder is essential. XP is composed of seven genetic complementation groups, XP-A to -G, and a variant type (XP-V). To establish an easy and accurate diagnosis of the eight disease groups, we constructed recombinant adenoviruses that expressed one of the XP cDNA. When fibroblasts derived from patients with XP-A, -B, -C, -D, -F or -G were infected with the adenovirus expressing XPA, XPB, XPC, XPD, XPF or XPG, respectively, and UV-C at 5-20 J/m 2 was irradiated, cell viability was clearly recovered by the corresponding recombinant adenoviruses. In contrast, XP-E and XP-V cells were not significantly sensitive to UV irradiation and were barely complemented by the matched recombinant adenoviruses. However, co-infection of Ad-XPA with Ad-XPE increased survival rate of XP-E cells after UV-C exposure. When XP-V cell strains, including one derived from a Japanese patient, were infected with Ad-XPV, exposed to UV-B and cultured with 1 mmol/L of caffeine, flow cytometry detected a characteristic decrease in the S phase in all the XP-V cell strains. From these results, the eight groups of XP could be differentiated by utilizing a set of recombinant adenoviruses, indicating that our procedure provides a convenient and correct diagnostic method for all the XP groups including XP-E and XP-V. © 2016 Japanese Dermatological Association.

  2. Human adenovirus serotype 12 virion precursors pMu and pVI are cleaved at amino-terminal and carboxy-terminal sites that conform to the adenovirus 2 endoproteinase cleavage consensus sequence.

    Science.gov (United States)

    Freimuth, P; Anderson, C W

    1993-03-01

    The sequence of a 1158-base pair fragment of the human adenovirus serotype 12 (Ad12) genome was determined. This segment encodes the precursors for virion components Mu and VI. Both Ad12 precursors contain two sequences that conform to a consensus sequence motif for cleavage by the endoproteinase of adenovirus 2 (Ad2). Analysis of the amino terminus of VI and of the peptide fragments found in Ad12 virions demonstrated that these sites are cleaved during Ad12 maturation. This observation suggests that the recognition motif for adenovirus endoproteinases is highly conserved among human serotypes. The adenovirus 2 endoproteinase polypeptide requires additional co-factors for activity (C. W. Anderson, Protein Expression Purif., 1993, 4, 8-15). Synthetic Ad12 or Ad2 pVI carboxy-terminal peptides each permitted efficient cleavage of an artificial endoproteinase substrate by recombinant Ad2 endoproteinase polypeptide.

  3. Estructura cambiaria alternativa en Venezuela (1960-2013: implicaciones y consecuencias

    Directory of Open Access Journals (Sweden)

    Héctor Romero

    2016-05-01

    Full Text Available El tipo de cambio nominal es un elemento clave dentro de la economía, ya que sirve como referencia básica para el intercambio de bienes entre los países, así como para la asignación de recursos productivos entre los distintos sectores económicos que componen la economía del país. En términos generales, las naciones del mundo suelen tener una única referencia cambiaria, sin embargo, bajo condiciones particulares de tensión en la balanza de pagos o severas crisis internas, pueden establecer estructuras cambiarias híbridas o múltiples. El trabajo tiene como objetivo comprender la dinámica de las estructuras cambiarias alternativas, las razones que conllevan a su instauración, así como la experiencia de la historia económica de Venezuela (1960-2013. El establecimiento de referencias cambiarias múltiples en el país suele estar asociado con la merma en la disponibilidad de activos externos, producto de choques negativos en los términos de intercambio o desajustes macroeconómicos financiados a través de la emisión monetaria. El aumento de la prima cambiaria entre el tipo de cambio oficial y el paralelo, con las consiguientes distorsiones en el funcionamiento de la economía nacional, ha sido el detonante principal para unificar el tipo de cambio. En consonancia con la experiencia internacional, la tasa de inflación promedio en los años con tipo de cambio unificado en Venezuela fue significativamente menor (14,5% que en los períodos con dos o más tipos de cambio (28,4%.

  4. Covalent decoration of adenovirus vector capsids with the carbohydrate epitope αGal does not improve vector immunogenicity, but allows to study the in vivo fate of adenovirus immunocomplexes.

    Science.gov (United States)

    Kratzer, Ramona F; Espenlaub, Sigrid; Hoffmeister, Andrea; Kron, Matthias W; Kreppel, Florian

    2017-01-01

    Adenovirus-based vectors are promising tools for genetic vaccination. However, several obstacles have to be overcome prior to a routine clinical application of adenovirus-based vectors as efficacious vectored vaccines. The linear trisaccharide epitope αGal (alpha-Gal) with the carbohydrate sequence galactose-α-1,3-galactosyl-β-1,4-N-acetylglucosamine has been described as a potent adjuvant for recombinant or attenuated vaccines. Humans and α-1,3-galactosyltransferase knockout mice do not express this epitope. Upon exposure of α-1,3-galactosyltransferase-deficient organisms to αGal in the environment, large amounts of circulating anti-Gal antibodies are produced consistently. Immunocomplexes formed between recombinant αGal-decorated vaccines and anti-Gal antibodies exhibit superior immunogenicity. We studied the effects of the trisaccharide epitope on CD8 T cell responses that are directed specifically to vector-encoded transgenic antigens. For that, covalently αGal-decorated adenovirus vectors were delivered to anti-Gal α-1,3-galactosyltransferase knockout mice. We generated replication-defective, E1-deleted adenovirus type 5 vectors that were decorated with αGal at the hexon hypervariable regions 1 or 5, at fiber knob, or at penton base. Surprisingly, none of the adenovirus immunocomplexes being formed from αGal-decorated adenovirus vectors and anti-Gal immunoglobulins improved the frequencies of CD8 T cell responses against the transgenic antigen ovalbumin. Humoral immunity directed to the adenovirus vector was neither increased. However, our data indicated that decoration of Ad vectors with the αGal epitope is a powerful tool to analyze the fate of adenovirus immunocomplexes in vivo.

  5. Videojuego con Realidad Virtual

    OpenAIRE

    González Mora, César

    2017-01-01

    El objetivo del proyecto es el desarrollo de un videojuego deportivo que utilice realidad mixta. El videojuego se podrá utilizar con dispositivos de tipo cardboard, y utilizará realidad aumentada para la interacción del jugador con el videojuego. En el desarrollo se utilizará el motor Unity para conseguir una aplicación multiplataforma, y la librería Vuforia para implementar realidad mixta.

  6. Sistemas integrados con Arduino

    OpenAIRE

    EL YAKOUTI, MOHAMMED

    2017-01-01

    Design of a robot prototype remotely controllable from Bluetooth using Arduino. Control and testing of sensors and events interacting with Arduino and Bluetooth. Diseño de un prototipo de robot controlable remotamente con Bluetooth utilizando Arduino. Control y verificación de los sensores y eventos que interactúan mediante el Arduino y el Bluetooth. El Yakouti, M. (2017). Sistemas integrados con Arduino. http://hdl.handle.net/10251/89274. TFGM

  7. Espacio Europeo de Educación Superior e implicaciones para las titulaciones de Magisterio en España

    Directory of Open Access Journals (Sweden)

    Juan José MONGE MIGUEL

    2005-01-01

    Full Text Available En esta comunicación se presenta la evolución de la Convergencia Europea de la Educación Superior (Proceso de Bolonia en su doble vertiente: política basada en las sucesivas declaraciones de los Ministros de Educación de los diferentes países de la Unión Europea y académica, por otra parte, respuestas o pasos dados fundamentalmente por las universidades a las declaraciones anteriores. En su última parte se trata de las implicaciones y planteamientos para la titulaciones de Magisterio en España, a la vez que se insiste en las dificultades e incluso crisis que los cambios (reformadores e innovadores que supone esta convergencia puede conllevar para la enseñanza superior europea.

  8. Eradication of melanoma in vitro and in vivo via targeting with a Killer-Red-containing telomerase-dependent adenovirus.

    Science.gov (United States)

    Takehara, Kiyoto; Yano, Shuya; Tazawa, Hiroshi; Kishimoto, Hiroyuki; Narii, Nobuhiro; Mizuguchi, Hiroyuki; Urata, Yasuo; Kagawa, Shunsuke; Fujiwara, Toshiyoshi; Hoffman, Robert M

    2017-08-18

    Melanoma is a highly recalcitrant cancer and transformative therapy is necessary for the cure of this disease. We recently developed a telomerase-dependent adenovirus containing the fluorescent protein Killer-Red. In the present report, we first determined the efficacy of Killer-Red adenovirus combined with laser irradiation on human melanoma cell lines in vitro. Cell viability of human melanoma cells was reduced in a dose-dependent and irradiation-time-dependent manner. We used an intradermal xenografted melanoma model in nude mice to determine efficacy of the Killer-Red adenovirus. Intratumoral injection of Killer-Red adenovirus, combined with laser irradiation, eradicated the melanoma indicating the potential of a new paradigm of cancer therapy.

  9. The evaluation of hollow-fiber ultrafiltration and celite concentration of enteroviruses, adenoviruses and bacteriophage from different water matrices

    Data.gov (United States)

    U.S. Environmental Protection Agency — The data to support the evaluation of hollow-fiber ultrafiltration and celite concentration of enteroviruses, adenoviruses and bacteriophage from different water...

  10. Development and Pre-Clinical Evaluation of a Novel Prostate-Restricted Replication Competent Adenovirus-AD-IU-1

    National Research Council Canada - National Science Library

    Gardner, Thomas A

    2006-01-01

    ... independent prostate cancers. The goal of this research is to develop a novel therapeutic agent, Ad-IU-1, using PSES to control the replication of adenovirus and the expression of a therapeutic gene, herpes simplex thymidine kinase (TK...

  11. Development and Pre-Clinical Evaluation of a Novel Prostate-Restricted Replication Competent Adenovirus-AD-IU-1

    National Research Council Canada - National Science Library

    Gardner, Thomas

    2004-01-01

    ... independent prostate cancers. The goal of this research is to develop a novel therapeutic agent, Ad-lu-1, using PSES to control the replication of adenovirus and the expression of a therapeutic gene, herpes simplex thymidine kinase...

  12. Development and Pre-Clinical Evaluation of a Novel Prostate-Restricted Replication Competent Adenovirus-Ad-IU-1

    National Research Council Canada - National Science Library

    Gardner, Thomas A

    2005-01-01

    .... The goal of this research is to develop a novel therapeutic agent, Ad-IU-1, using PSES to control the replication of adenovirus and the expression of a therapeutic gene, herpes simplex thymidine kinase (TK...

  13. Investigando con personas con dificultades de aprendizaje

    Directory of Open Access Journals (Sweden)

    Borja González Luna

    2013-12-01

    Full Text Available El artículo muestra los orígenes de lo que Walmsley (2008 denomina «investigación inclusiva». Para comprender qué se entiende por investigación inclusiva tenemos que remontarnos a los debates epistemológicos sobre las metodologías cuantitativas y cualitativas, acontecidos en la década de los 90, en torno a la revista Disability & Society. A partir de una síntesis de dichos debates, focalizados en el ámbito de la «discapacidad intelectual y del desarrollo», se exponen dos estrategias de colaboración con dicha población: a una aproximación etnográfica (de trabajo grupal, y b una aproximación biográfica (de trabajo individual. A continuación se esboza un posible diseño de trabajo de campo que intenta superar el paradigma cualitativo «clásico» con el objetivo de incluir a dicho colectivo más allá del rol de «sujetos de la investigación». Para finalizar se recoge el debate sobre la accesibilidad de los resultados de la investigación a los participantes en dichas investigaciones, y con ello la necesaria innovación en el ámbito de las «devoluciones» de los resultados, cuando se trata de incluir a personas que presentan limitaciones para la comprensión del lenguaje abstracto oral y/o escrito.

  14. Implicaciones del marco teórico de lo imaginario en la Psicología Social

    Directory of Open Access Journals (Sweden)

    Teresa Aracena Vicente

    2015-03-01

    Full Text Available En el presente estudio abordaremos dos de las principales concepciones sobre los mecanismos implicados en la construcción significativa del mundo. Hablamos de la Teoría de las Representaciones Sociales (TRS, formulada desde la psicología, y del marco teórico de lo imaginario, formulado principalmente desde la antropología y la filosofía. Procederemos exponiendo, primero, la TRS y, posteriormente, los acercamientos al marco teórico de lo imaginario desde diferentes disciplinas, para señalar las aportaciones que ofrece este último a la psicología social, aportaciones éstas que complementan las de la TRS. Así, este estudio tiene como objetivos realizar un análisis teórico de la TRS y del marco teórico de lo imaginario y especificar las contribuciones e implicaciones del marco teórico de lo imaginario a la psicología social (y, más concretamente, a la psicología cultural de modo a observar cómo el concepto de imaginario social incluye y trasciende al de representación social(RS. Consideraremos las implicaciones ontológicas, epistemológicas, metodológicas y para la salud de la utilización del marco teórico de lo imaginario en la psicología cultural. En este sentido, propondremos la adopción de dicho concepto por la psicología, considerando que el mismo es fundamental por su capacidad explicativa, comprensiva, interpretativa y crítica.

  15. Viable adenovirus vaccine prototypes: High-level production of a papillomavirus capsid antigen from the major late transcriptional unit

    OpenAIRE

    Berg, Michael; DiFatta, Julie; Hoiczyk, Egbert; Schlegel, Richard; Ketner, Gary

    2005-01-01

    Safe, effective, orally delivered, live adenovirus vaccines have been in use for three decades. Recombinant derivatives of the live adenovirus vaccines may prove an economical alternative to current vaccines for a variety of diseases. To explore that possibility, we constructed a series of recombinants that express the major capsid protein (L1) of canine oral papillomavirus (COPV), a model for mucosal human papillomavirus (HPV) infection. Vaccination with virus-like particles (VLPs) composed ...

  16. Las ideologias, las ciencias naturales y sus implicaciones en la educacion cientifica

    Science.gov (United States)

    Lozada Roldan, Sandra

    Este estudio ausculto las concepciones epistemologicas de los docentes de ciencia del nivel secundario con relacion a las ideologias y las ciencias naturales. Tambien examino las posiciones de los docentes ante asuntos publicos relacionados a la ciencia. Para propositos de este estudio se diseno y se valido el cuestionario con el cual se obtuvieron los resultados. La investigacion es de tipo cuantitativa y se utilizo como diseno la encuesta. El cuestionario se administro en varias actividades de desarrollo profesional para maestros de ciencia. Un total de 78 maestros del nivel secundario respondieron el cuestionario. Para analizar los datos obtenidos se utilizaron estadisticas descriptivas como la distribucion de frecuencia y el porciento. Ademas se establecieron codigos y categorias para describir las posiciones de los maestros ante asuntos publicos relacionados a la ciencia. Los analisis demostraron que entre los docentes participantes de este estudio prevalecen ciertas concepciones epistemologicas adecuadas acerca de las ciencias naturales, a la luz de la literatura consultada. Entre estas concepciones se destacan las siguientes: a) la filosofia materialista de las ciencias naturales, b) la naturaleza tentativa y constructivista del conocimiento cientifico, c) el uso de una metodologia que garantiza cierto grado de objetividad y con el que se justifican y validan los enunciados cientificos y d) la funcion instrumental del conocimiento cientifico. Sin embargo, entre los docentes participantes de este estudio prevalecen ciertas concepciones epistemologicas erroneas acerca de las ciencias naturales, a la luz de la literatura consultada. Entre estas concepciones se destacan las siguientes: a) tendencia inductivista en el que las teorias cientificas comienzan con observaciones que establecen generalizaciones, b) secuencia jerarquica de la metodologia cientifica. Ademas, entre los docentes participantes de este estudio prevalecen concepciones epistemologicas adecuadas

  17. Calidad de vida y estrategias de afrontamiento en pacientes con insuficiencia renal crónica sometidos a hemodiálisis, diálisis peritoneal o trasplante renal

    Directory of Open Access Journals (Sweden)

    Paola Andrea Acosta Hernández

    2008-01-01

    Full Text Available Se comparó la calidad de vida y las estrategias de afrontamiento en 120 pacientes con Insuficiencia Renal Crónica sometidos a hemodiálisis, diálisis peritoneal o trasplante renal, teniendo en cuenta la edad, sexo, escolaridad, estado civil y ocupación. Los instrumentos utilizados fueron una ficha sociodemográfica, la prueba de calidad de vida WHOQOL-100 (Organización Mundial de la Salud, 1998 y el Cuestionario de Estrategias de Afrontamiento (Lazarus & Folkman, 1985. Se encontró que los pacientes en tratamiento de trasplante reportaban mayor calidad de vida y mejores estrategias de afrontamiento en comparación con los otros tratamientos, siendo mayoreslas puntuaciones en personas con edad de transición entre adultez media y tardía, mujeres, con estudios universitarios, casados y empleados. Se discuten las implicaciones de estos hallazgos.

  18. Inhibition of TRAIL-induced apoptosis and forced internalization of TRAIL receptor 1 by adenovirus proteins.

    Science.gov (United States)

    Tollefson, A E; Toth, K; Doronin, K; Kuppuswamy, M; Doronina, O A; Lichtenstein, D L; Hermiston, T W; Smith, C A; Wold, W S

    2001-10-01

    Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis through two receptors, TRAIL-R1 (also known as death receptor 4) and TRAIL-R2 (also known as death receptor 5), that are members of the TNF receptor superfamily of death domain-containing receptors. We show that human adenovirus type 5 encodes three proteins, named RID (previously named E3-10.4K/14.5K), E3-14.7K, and E1B-19K, that independently inhibit TRAIL-induced apoptosis of infected human cells. This conclusion was derived from studies using wild-type adenovirus, adenovirus replication-competent mutants that lack one or more of the RID, E3-14.7K, and E1B-19K genes, and adenovirus E1-minus replication-defective vectors that express all E3 genes, RID plus E3-14.7K only, RID only, or E3-14.7K only. RID inhibits TRAIL-induced apoptosis when cells are sensitized to TRAIL either by adenovirus infection or treatment with cycloheximide. RID induces the internalization of TRAIL-R1 from the cell surface, as shown by flow cytometry and indirect immunofluorescence for TRAIL-R1. TRAIL-R1 was internalized in distinct vesicles which are very likely to be endosomes and lysosomes. TRAIL-R1 is degraded, as indicated by the disappearance of the TRAIL-R1 immunofluorescence signal. Degradation was inhibited by bafilomycin A1, a drug that prevents acidification of vesicles and the sorting of receptors from late endosomes to lysosomes, implying that degradation occurs in lysosomes. RID was also shown previously to internalize and degrade another death domain receptor, Fas, and to prevent apoptosis through Fas and the TNF receptor. RID was shown previously to force the internalization and degradation of the epidermal growth factor receptor. E1B-19K was shown previously to block apoptosis through Fas, and both E1B-19K and E3-14.7K were found to prevent apoptosis through the TNF receptor. These findings suggest that the receptors for TRAIL, Fas ligand, and TNF play a role in limiting virus

  19. Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production.

    Science.gov (United States)

    Ferreira, Tiago B; Ferreira, Ana L; Carrondo, Manuel J T; Alves, Paula M

    2005-11-01

    Adenoviruses are promising vectors for gene therapy and vaccination protocols. Consequently, the market demands for adenovirus are increasing, driving the search for new methodologies for large-scale production of concentrated vectors with warranted purity and efficacy, in a cost-effective way. Nevertheless, the production of adenovirus is currently limited by the so-called 'cell density effect', i.e. a drop in cell specific productivity concomitant with increased cell concentration at infection. Of two different serum-free culture media (CD293 and EX-Cell), evaluated for their effect on human 293 cells growth and adenovirus production at cell densities higher than 1x10(6) cells/ml, EX-Cell proved the better medium for cell growth. Although adenovirus production was equivalent in both media when the infection was performed at 1x10(6) cells/ml, at 3x10(6) cells/ml CD293 was the better. This result related to the high ammonia content in EX-Cell medium at the highest cell concentration at infection. Besides this, the large-scale production of these vectors at high cell densities often requires re-feed strategies, which increase medium osmolality. While a negative effect on cell growth was observed with increasing osmolalities, adenovirus productivity was only affected for osmolalities higher than 430 mOsm.

  20. Censo estadounidense 2010: cifras e implicaciones de la mayor presencia de Centroamericanos en Estados Unidos

    Directory of Open Access Journals (Sweden)

    José Luis Rocha

    2011-12-01

    Full Text Available Con el tic-tac del reloj del censo estadounidense, este texto da cuenta de las cifras de migrantes centroamericanos en Estados Unidos: cuántos son, a qué ritmo crecen, dónde se ubican. Posteriormente, con base en el procesamiento de las estadísticas del Census Bureau 2010, muestra algunas características de los centroamericanos: son los más rezagados -en educación, arraigo, ingresos, obtención de ciudadanía, y de empleos estables y bien remunerados- entre los inmigrantes latinos. Sus patrones de asentamiento pueden repercutir negativamente sobre su futuro porque sus opciones geográficas son un voto político de los sin voto: contribuyen al crecimiento demográfico y de sillas en el congreso de los estados sureños, proverbial plaza de republicanos que han promovido leyes y operativos anti-inmigrantes.

  1. Interacciones humano-animal: características e implicaciones para el bienestar de los humanos

    Directory of Open Access Journals (Sweden)

    Natalia Piar

    2007-07-01

    Full Text Available En este artículo se revisa la literatura reciente sobre interacciones humano-animal, su origen, sus características y cómo dichas interacciones afectan el bienestar físico, psicológico y social. En la primera parte se presentan los orígenes y evolución histórica de las relaciones humano - animal, empezando por los procesos de domesticación y se aborda la naturaleza de las interacciones humano animal. En la segunda parte, se revisan los efectos para los humanos de la interacción con animales, especialmente con sus mascotas y se analiza la literatura científica sobre los efectos físicos, psicológicos y sociales. Finalmente, se aborda brevemente el desarrollo de esta área de investigación en Latinoamérica.

  2. Implicaciones anestésicas de un caso de leucodistrofia metacromática

    OpenAIRE

    Gupta, Arun Kumar; Mahajan, Charu; Mehta, Anjali; Dhulkhed, Vithal K

    2010-01-01

    La leucodistrofia metacromática es una enfermedad progresiva hereditaria y neurodegenerativa. Un paciente que sufre de esta enfermedad representa múltiples problemas anestésicos. Hemos anestesiado exitosamente una niña que sufre de leucodistrofia metacromática con anestesia general. Metachromatic leukodystrophy is a progressive, inherited and neurodegenerative disease. A patient suffering from this disease poses a lot of anaesthetic problems. We have successfully anaesthetized a female chi...

  3. Desarrollo turístico e implicaciones medioambientales en Puerto Vallarta

    Directory of Open Access Journals (Sweden)

    Javier Orozco Alvarado

    2016-02-01

    Full Text Available A escala nacional e internacional, la actividad turística es reconocida como un importante eje de desarrollo. Sin embargo, dicho sector económico debe ser congruente con los principios y postulados del desarrollo sustentable. En Puerto vallarta estamos lejos de lograr el turismo sustentable, por lo que urge frenar el deterioro e implementar acciones en beneficio del futuro del destino turístico y de todos quienes en él habitan y hacen negocio.

  4. Tratamiento endoluminal de la aorta. Implicaciones en la enfermedad de la aorta torácica

    Directory of Open Access Journals (Sweden)

    Miguel Josa García-Tornel

    2007-10-01

    Full Text Available Durante los últimos años el tratamiento quirúrgico endoluminal de la enfermedad de la aorta (EVAR, endovascular aortic repair se ha convertido en una opción terapéutica en un espectro amplio de pacientes. EVAR es una técnica relativamente fácil, menos agresiva y con aparente menor riesgo con respecto a la cirugía convencional de la aorta. La evidente presión de la industria, a menudo combinada con el lógico interés por su desarrollo por parte de muchos grupos clínicos y el factor moda, tan en boga en nuestros tiempos, han dado a la cirugía endoluminal de la aorta abdominal un desarrollo muy rápido que se está extendiendo en la actualidad a segmentos de la aorta torácica. Aunque los resultados publicados parecen avalar un papel importante de EVAR en algunos grupos específicos de pacientes, su uso en muchos otros grupos es muy controvertido. Su alto coste y los resultados a medio plazo parecen poner en tela de juicio la tendencia a la generalización que existe hoy día. La aplicación a la aorta torácica está asociada con un mayor número de interrogantes. Estudios rigurosos y mejoras técnicas ayudarán, sin duda, a definir mejor su indudable aplicabilidad en el tratamiento de la aorta tanto abdominal como torácica, pero en la actualidad su uso debe estar regulado por un cumplimiento estricto de indicaciones basadas en evidencias clínicas y alejadas de otros intereses, y su práctica debe reducirse a grupos de multiespecialidad preparados en todos los aspectos de la cirugía cardiovascular.

  5. Learning to live with data deluge and what that means for educators ( Aprendiendo a vivir con el aluvion informative y sus informative y sus implicaciones para la educacion),

    OpenAIRE

    Jones, B; Goff, M.

    2011-01-01

    A major characteristic of our age is the exponential rise of data. Vast quantities of information in digital form are both consumed by and generated from research. The internet has made “information” accessible to those with the resources to access and use the Internet. As a consequence a new set of requirements has been generated for educational institutions to equip both their teachers and learners with the information skills to access and effectively exploit this digital environment for le...

  6. Aspectos moleculares del virus de la parvovirosis canina y sus implicaciones en la enfermedad

    Directory of Open Access Journals (Sweden)

    César A. Díaz R.

    2008-06-01

    Full Text Available El parvovirus canino tipo 2 (CPV-2 es el agente causal de una enfermedad infecto-contagiosa que produce una gastroenteritis aguda hemorrágica que afecta a caninos jóvenes. El CPV-2 se adaptó a la especie canina por mutación del virus de la Panleucopenia felina (FPV luego de su paso por animales silvestres como el hurón y los zorros. La alta variabilidad de la proteína viral 2 (VP2 es la causa principal del amplio rango de hospedadores y de las reacciones cruzadas entre las variantes. En la actualidad, la secuenciación de esta proteína ha permitido identificar tres variantes del virus conocidas como 2a, 2b y 2c que conviven en el mundo con diferencias en tropismo celular, infecciocidad y patogenicidad. El virus ssADN ha presentado una gran variación génica en cortos períodos de tiempo lo que indica un alto grado de selección por evolución sólo comparable con virus RN, esta alta variabilidad no se ha aclarado totalmente. El empleo de las técnicas moleculares permitirá diferenciar entre cepas vacunales y de campo, tanto como contar con técnicas diagnósticas confiables y específicas.

  7. El conductismo y algunas implicaciones de lo que significa ser conductista hoy

    Directory of Open Access Journals (Sweden)

    Camilo Hurtado

    2006-01-01

    Full Text Available Se discuten varios aspectos relacionados con el conductismo, sus múltiples versiones, una definición amplia de éste y a quienes se les puede denominar actualmente “conductistas”. Además, a partir de una anécdota se analiza, en términos de barreras epistemológicas, el rechazo a esta tradición de pensamiento y se discute como dicho rechazo está enraizado y mantenido en una serie de malentendidos y en las contradicciones que existen entre los principios del conductismo genérico y los de la llamada “resto de la psicología” o “mainstream psychology” la cual se caracteriza por diversas formas de cognoscitivismo y ser muy afín con la que se ha llamado “psicología popular”. Finalmente, se analizan algunas propuestas que han sido sugeridas para superar las barreras epistemológicas del conductismo, concluyendo que el contexto académico actual no es el apropiado para que estos intentos cumplan con su finalidad, en cambio, al parecer dicho contexto es fuente en sí mismo de otro tipo de barreras.

  8. Estudio del rendimiento en 3 dimensiones neurocognitivas mediante nuevas tecnologías de tipo táctil en una población de niños/as diagnosticados/as con TDAH : diferencias entre subtipos clínicos

    OpenAIRE

    Fenollar Cortés, Javier

    2014-01-01

    El Trastorno por Déficit de Atención con Hiperactividad (TDAH) es uno de los trastornos neurológicos más frecuentes en la infancia, y cuyas implicaciones clínicas afectan a las principales áreas de la vida relacionadas con un adecuado desarrollo. No obstante, a pesar de la amplia literatura del TDAH de la que disponemos, sigue considerándose una realidad compleja y en permanente revisión. Ha sido una constante en el estudio del TDAH explorar los déficits cognitivos que subyacen al trastorn...

  9. The urethral smear as a tool in diagnosing adenovirus-induced urethritis.

    Science.gov (United States)

    Tønsberg, E; Hartgill, U

    2014-12-01

    Adenovirus is a recognised cause of non-gonococcal urethritis, and is not uncommonly associated with extragenital signs and symptoms. This case report describes a patient with symptoms of conjunctivitis, meatitis and urethritis. The urethral smear revealed almost exclusively monocytes microscopically, raising the suspicion of a viral aetiology. Results confirmed the presence of adenovirus in both the eyes and urethra. Despite waning reliance on the urethral smear in sexual health clinics, it can still be an important diagnostic tool in assessing the aetiology of non-specific urethritis. Finding an obvious monocytic cell response in the urethral smear can indicate a viral cause and allow the clinician to optimise management, counsel appropriately, and potentially reduce unnecessary antibiotic use. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  10. Accumulation of infectious mutants in stocks during the propagation of fiber-modified recombinant adenoviruses

    International Nuclear Information System (INIS)

    Ugai, Hideyo; Inabe, Kumiko; Yamasaki, Takahito; Murata, Takehide; Obata, Yuichi; Hamada, Hirofumi; Yokoyama, Kazunari K.

    2005-01-01

    In infected cells, replication errors during viral proliferation generate mutations in adenoviruses (Ads), and the mutant Ads proliferate and evolve in the intracellular environment. Genetically fiber-modified recombinant Ads (rAd variants) were generated, by modification of the fiber gene, for therapeutic applications in host cells that lack or express reduced levels of the Coxsackievirus and adenovirus receptor. To assess the genetic modifications of rAd variants that might induce the instability of Ad virions, we examined the frequencies of mutants that accumulated in propagated stocks. Seven of 41 lines of Ad variants generated mutants in the stocks and all mutants were infectious. Moreover, all the mutations occurred in the modified region that had been added at the 3' end of the fiber gene. Our results show that some genetic modifications at the carboxyl terminus of Ad fiber protein lead to the instability of Ad virions

  11. Establishment of an agamid cell line and isolation of adenoviruses from central bearded dragons (Pogona vitticeps).

    Science.gov (United States)

    Ball, Inna; Hoferer, Marc; Marschang, Rachel E

    2014-03-01

    A cell line was established from whole 6-8-week-old central bearded dragon (Pogona vitticeps) embryos. Cells were mid-sized and showed an elongated and polymorphic form. The cell line grew in a monolayer and has been serially passaged for 17 passages at time of publication. This cell line has been used with samples from adenovirus polymerase chain reaction (PCR)-positive bearded dragons, and 2 virus isolates have been obtained so far. The isolates show a clear cytopathic effect in inoculated cells. Both virus isolates have been serially passaged on this cell line, and have been identified by PCR amplification and sequencing of a portion of the DNA-dependent DNA polymerase gene and show 100% nucleotide identity to the corresponding region of an agamid adenovirus. Electron microscopic examination of supernatant from infected cells demonstrated the presence of nonenveloped particles, with a diameter of approximately 80 nm in both virus isolates.

  12. A retrospective investigation of canine adenovirus (CAV infection in adult dogs in Turkey : article

    Directory of Open Access Journals (Sweden)

    S. Gur

    2009-05-01

    Full Text Available Canine adenovirus (CAV type 1 and 2, respectively, cause infectious canine hepatitis and infectious canine laryngotracheitis in members of the families Canidae and Ursidae worldwide. Both of these infections are acute diseases, especially in young dogs. The aim of this study was to conduct a serological investigation of canine adenovirus infection. For this purpose, serumsamples were collected from native pure-bred Kangal (n = 11, and Akbash dogs (n = 17 and Turkish Greyhounds (n=15 in Eskisehir and Konya provinces. None ofthe dogs were previously vaccinated against CAV types. Indirect ELISA detected 88.2 %, 93.3 % and 100 % prevalences in Akbash, Greyhound and Kangal dogs, respectively. The remainder of the samples (n = 51 were collected at the Afyonkarahisar Municipality Shelter. Fourty-two of these dogs (82.3 % were detected as seropositive. In total, 82 of 94 dogs (87.2 % were found to be positive for CAV serum antibodies.

  13. Rapid and sustained CD4(+) T-cell-independent immunity from adenovirus-encoded vaccine antigens

    DEFF Research Database (Denmark)

    Holst, Peter J; Bartholdy, Christina; Buus, Anette Stryhn

    2007-01-01

    -linked lymphocytic choriomeningitis virus (LCMV)-derived epitopes was long-lived and protective. Notably, in contrast to full-length protein, the response elicited with the beta(2)-microglobulin-linked LCMV-derived epitope was CD4(+) T-cell independent. Furthermore, virus-specific CD8(+) T cells primed...... in the absence of CD4(+) T-cell help were sustained in the long term and able to expand and control a secondary challenge with LCMV. Our results demonstrate that modifications to the antigen used in adenovirus vaccines may be used to improve the induced T-cell response. Such a strategy for CD4(+) T-cell...... to that elicited with an adenovirus-encoded minimal epitope covalently linked to beta(2)-microglobulin. We demonstrate that the beta(2)-microglobulin-linked epitope induced an accelerated and augmented CD8(+) T-cell response. Furthermore, the immunity conferred by vaccination with beta(2)-microglobulin...

  14. Investigar con y sobre los jóvenes colaborando y educando

    Directory of Open Access Journals (Sweden)

    María Domingo Coscollola

    2014-01-01

    Full Text Available Este artículo da cuenta de una investigación colaborativa realizada con y sobre los jóvenes. En este trabajo, cinco grupos de estudiantes de cuarto de Educación Secundaria Obligatoria (ESO, de otros tantos centros de Cataluña, han realizado cinco estudios etnográficos de forma colaborativa entre ellos, algunos de sus docentes y miembros de nuestro equipo de investigación, con la finalidad de explorar cómo y con qué los jóvenes se comunican, expresan y aprenden dentro y fuera de las instituciones educativas. Comienza discutiendo las dimensiones de la colaboración en la educación, teniendo en cuenta las aportaciones del aprendizaje colaborativo y cooperativo y las potencialidades de los recursos digitales, y situando los antecedentes y las particularidades del trabajo llevado a cabo. Sigue con la caracterización de cómo y en qué ha consistido la investigación con los jóvenes a los que invitamos a ejercer como investigadores. Los resultados se centran en la descripción y conceptualización de las formas de colaboración a las que ha dado lugar la producción de estos cinco estudios etnográficos en cada uno de los centros utilizando tecnologías digitales. Finalmente, se discuten las implicaciones del trabajo realizado y se señalan sus limitaciones lo que se configura como la principal aportación para quienes se propongan investigar con y sobre los jóvenes colaborando, educando y utilizando recursos digitales.

  15. Assessment of the Incidence of Enteric Adenovirus Species and Serotypes in Surface Waters in the Eastern Cape Province of South Africa: Tyume River as a Case Study

    Directory of Open Access Journals (Sweden)

    Timothy Sibanda

    2012-01-01

    Full Text Available TaqMan real-time PCR was used for the detection and quantitation of adenoviruses in Tyume River water samples over a 12-month period. A total of 72 samples were analysed, and 22 samples were positive for adenovirus. Of the positive samples, 18 were collected from downstream sampling points. Among the downstream sampling points, adenovirus detection rate increased with distance downstream, being 28%, 33%, and 39% for Alice, Drayini, and Manqulweni, respectively. The Alice sampling site had the highest concentrations of adenovirus ranging between 6.54×103 genome copies/L and 8.49×104 genome copies/L. The observed trend could have been expected considering the level of anthropogenic activities in areas along the lower stretch of Tyume River, with the major one being the effluent of treated and semi treated sewage from wastewater treatment facilities. Adenovirus detection was sporadic at most sampling sites. Multiplex conventional PCR was used for the detection of clinically important adenovirus species B, C, and F and their serotypes. Species C and F adenoviruses were detected in 77% and 18% of the samples, respectively. Most adenovirus positive samples were obtained from areas of increased population densities. The presence of adenoviruses may confirm the risk of its transmission to the human population.

  16. MicroRNA-mediated suppression of oncolytic adenovirus replication in human liver.

    Directory of Open Access Journals (Sweden)

    Erkko Ylösmäki

    Full Text Available MicroRNAs (miRNAs are important and ubiquitous regulators of gene expression that can suppress their target genes by translational inhibition as well as mRNA destruction. Cell type-specific miRNA expression patterns have been successfully exploited for targeting the expression of experimental and therapeutic gene constructs, for example to reduce pathogenic effects of cancer virotherapy in normal tissues. In order to avoid liver damage associated with systemic or intrahepatic delivery of oncolytic adenoviruses we have introduced the concept of suppressing adenovirus replication in hepatic cells by inserting target elements for the liver-specific miR122 into the viral genome. Here we show using ex vivo cultured tissue specimens that six perfectly complementary miR122 target sites in the 3' untranslated region of the viral E1A gene are sufficient in the absence of any other genetic modifications to prevent productive replication of serotype 5 adenovirus (Ad5 in normal human liver. This modification did not compromise the replicative capacity of the modified virus in cancer tissue derived from a colon carcinoma liver metastasis or its oncolytic potency in a human lung cancer xenograft mouse model. Unlike wild-type Ad5, the modified virus did not result in increased serum levels of liver enzymes in infected mice. These results provide a strong preclinical proof of concept for the use of miR122 target sites for reducing the risk of liver damage caused by oncolytic adenoviruses, and suggest that ectopic miR122 target elements should be considered as an additional safety measure included in any therapeutic virus or viral vector posing potential hazard to the liver.

  17. Adenovirus Detection by the cGAS/STING/TBK1 DNA Sensing Cascade

    Science.gov (United States)

    Lam, Eric; Stein, Saskia

    2014-01-01

    Adenovirus (Ad) infection triggers a cell-specific antiviral response following exposure of viral DNA to the intracellular compartment. A variety of DNA sensors (DAI, AIM2, DDx41, RNA polymerase [Pol] III, and IFI16 [p204]) have been identified in recent years; however, the DNA sensor involved in detection of adenovirus has not been established. Cyclic GMP-AMP synthase (cGAS), a DNA sensor that produces a cyclic guanine-adenine dinucleotide (cGAMP) inducer of STING, has been examined to determine its role in generating an antiadenoviral response. Short hairpin RNA (shRNA) lentiviral vectors targeting TBK1, STING, and cGAS were established in murine MS1 endothelial and RAW 264.7 macrophage cell lines. Knockdown of TBK1, STING, and cGAS results in a dramatic reduction in the activation of the primary antiviral response marker phosphorylated interferon (IFN) response factor 3 (IRF3) following exposure to adenovirus. Furthermore, activation of secondary type I IFN signaling targets (ptyrSTAT1 and ptyrSTAT2 [ptyrSTAT1/2]) was also compromised. Consistent with compromised activation of primary and secondary response markers, transcriptional activation of IRF3-responsive genes (beta IFN [IFN-β], ISG15, ISG54) and secondary response transcripts were diminished in cells knocked down in cGAS, STING, or TBK1. These data establish cGAS as the dominant cytosolic DNA sensor responsible for detection of internalized adenovirus leading to induction of the type I interferon antiviral cascade. PMID:24198409

  18. Immunogenicity and efficacy of a chimpanzee adenovirus-vectored Rift Valley fever vaccine in mice.

    OpenAIRE

    Warimwe, GM; Lorenzo, G; Lopez-Gil, E; Reyes-Sandoval, A; Cottingham, MG; Spencer, AJ; Collins, KA; Dicks, MD; Milicic, A; Lall, A; Furze, J; Turner, AV; Hill, AV; Brun, A; Gilbert, SC

    2013-01-01

    BACKGROUND: Rift Valley Fever (RVF) is a viral zoonosis that historically affects livestock production and human health in sub-Saharan Africa, though epizootics have also occurred in the Arabian Peninsula. Whilst an effective live-attenuated vaccine is available for livestock, there is currently no licensed human RVF vaccine. Replication-deficient chimpanzee adenovirus (ChAd) vectors are an ideal platform for development of a human RVF vaccine, given the low prevalence of neutralizing antibod...

  19. Immunogenicity and efficacy of a chimpanzee adenovirus-vectored Rift Valley fever vaccine in mice.

    Science.gov (United States)

    Warimwe, George M; Lorenzo, Gema; Lopez-Gil, Elena; Reyes-Sandoval, Arturo; Cottingham, Matthew G; Spencer, Alexandra J; Collins, Katharine A; Dicks, Matthew D J; Milicic, Anita; Lall, Amar; Furze, Julie; Turner, Alison V; Hill, Adrian V S; Brun, Alejandro; Gilbert, Sarah C

    2013-12-05

    Rift Valley Fever (RVF) is a viral zoonosis that historically affects livestock production and human health in sub-Saharan Africa, though epizootics have also occurred in the Arabian Peninsula. Whilst an effective live-attenuated vaccine is available for livestock, there is currently no licensed human RVF vaccine. Replication-deficient chimpanzee adenovirus (ChAd) vectors are an ideal platform for development of a human RVF vaccine, given the low prevalence of neutralizing antibodies against them in the human population, and their excellent safety and immunogenicity profile in human clinical trials of vaccines against a wide range of pathogens. Here, in BALB/c mice, we evaluated the immunogenicity and efficacy of a replication-deficient chimpanzee adenovirus vector, ChAdOx1, encoding the RVF virus envelope glycoproteins, Gn and Gc, which are targets of virus neutralizing antibodies. The ChAdOx1-GnGc vaccine was assessed in comparison to a replication-deficient human adenovirus type 5 vector encoding Gn and Gc (HAdV5-GnGc), a strategy previously shown to confer protective immunity against RVF in mice. A single immunization with either of the vaccines conferred protection against RVF virus challenge eight weeks post-immunization. Both vaccines elicited RVF virus neutralizing antibody and a robust CD8+ T cell response. Together the results support further development of RVF vaccines based on replication-deficient adenovirus vectors, with ChAdOx1-GnGc being a potential candidate for use in future human clinical trials.

  20. Intrahepatic injection of adenovirus reduces inflammation and increases gene transfer and therapeutic effect in mice

    OpenAIRE

    Crettaz, J. (Julien); Berraondo, P. (Pedro); Mauleon, I. (Itsaso); Ochoa, L. (Laura); Shankar, V. (Vijay); Barajas, M. (Miguel); Rooijen, N. (Nico) van; Kochanek, S. (Stefan); Qian, C. (Cheng); Prieto, J. (Jesús); Hernandez-Alcoceba, R. (Rubén); Gonzalez-Aseguinolaza, G. (Gloria)

    2006-01-01

    Recombinant adenoviruses (Ad) are among the most extensively used vectors for liver gene transfer. One of the major limitations for the clinical application of these vectors is the inflammatory immune response associated with systemic administration of high dose of virus. We evaluated the effect of Ad administration route on the inflammatory immune response and liver transgene expression. We compared direct intrahepatic injection (IH) with the systemic administration via tail vein (IV). IH in...

  1. Giochiamo con i robot

    Directory of Open Access Journals (Sweden)

    Andrea Bonarini

    2009-01-01

    Full Text Available "Giochiamo con i robot" e' un laboratorio interattivo per grandi e piccini realizzato per l'edizione 2007 del Festival della Scienza di Genova. Lungo un percorso che va dalla telerobotica alla robotica evolutiva, il laboratorio sviluppa il tema di dare intelligenza ai robot. Questo percorso, le cui tappe sono le varie installazioni, si conclude nella "bottega" dove e' possibile costruire e programmare i propri robot o smontare e modificare quelli esposti durante il percorso didattico. I visitatori sono coinvolti in attivita' ludiche grazie alle quali possonoentrare in contatto con alcune delle idee potenti della robotica,

  2. disegnare con ... Alberto Pratelli

    Directory of Open Access Journals (Sweden)

    Roberto Mingucci

    2017-12-01

    Full Text Available Con questa breve intervista ad Alberto Pratelli, (non a caso scelto per aprire questa nuova rubrica intendia-mo inaugurare un dialogo con personalità significati-ve del Disegno di Architettura, che consenta riflessioni dedicate alle sue varie dimensioni, oggi più che mai da approfondire. La suggestione a farlo, viene da un’idea di Pablo Rodri-guez Navarro ed abbiamo quindi pensato di avviarla proprio in questo numero, che Pablo ha accettato di curare su un tema a lui particolarmente caro.

  3. Acoso laboral y trastornos de la personalidad: un estudio con el MCMI-II

    Directory of Open Access Journals (Sweden)

    David González Trijueque

    2008-01-01

    Full Text Available En este artículo se lleva a cabo un estudio de los trastornos de personalidad asociados al acoso psicológico en el lugar de trabajo (mobbing. Para ello se contó con una muestra de 50 víctimas de acoso laboral, que cumplimentaron el MCMI-II, y de 50 sujetos de la población activa normal con las mismas características demográficas (edad, sexo, nivel socioeconómico. El 36% de la muestra clínica (frente al 14% de la muestra normativa presentaba, al menos, un trastorno de personalidad. El trastorno de personalidad de mayor prevalencia fue el trastorno compulsivo (18%, seguido del dependiente (8% y del fóbico-evitativo (4% e histriónico (4%. Las víctimas de mobbing con trastornos de personalidad presentaban además mayores niveles de ansiedad (rasgo y estado y mayor consumo de alcohol que los trabajadores acosados que no presentaban ningun trastorno de la personalidad. Por último, se comentan las implicaciones de este estudio para la práctica clínica y para las investigaciones futuras.

  4. Tipologías de Feminicidas con Trastorno Mental en España

    Directory of Open Access Journals (Sweden)

    Raúl Aguilar-Ruiz

    2018-01-01

    Full Text Available Los tratamientos terapéuticos en hombres maltratadores tienen una eficacia limitada. Una de las acciones propuestas para mejorar las intervenciones de los profesionales en este ámbito es adaptarlas a las características de cada maltratador. Con base en el estudio de 237 sentencias por feminicidio en España cometido por hombres con alteraciones mentales en el momento de los hechos, se pretende conocer las diferentes clases de feminicidas con trastorno mental. También se estudia la relación entre el trastorno mental y el grado de afectación psíquica y entre la conducta suicida y la ruptura de la relación de pareja. Los resultados obtenidos mediante técnicas estadísticas bivariantes y multivariantes revelan la existencia de cuatro tipologías de feminicidas. Asimismo, se ha hallado asociación positiva entre el trastorno mental y la intensidad de la afectación psíquica y entre la conducta suicida y el abandono de la mujer. Se describen las implicaciones teóricas y prácticas.

  5. Purification of infectious adenovirus in two hours by ultracentrifugation and tangential flow filtration

    International Nuclear Information System (INIS)

    Ugai, Hideyo; Yamasaki, Takahito; Hirose, Megumi; Inabe, Kumiko; Kujime, Yukari; Terashima, Miho; Liu, Bingbing; Tang, Hong; Zhao, Mujun; Murata, Takehide; Kimura, Makoto; Pan, Jianzhi; Obata, Yuichi; Hamada, Hirofumi; Yokoyama, Kazunari K.

    2005-01-01

    Adenoviruses are excellent vectors for gene transfer and are used extensively for high-level expression of the products of transgenes in living cells. The development of simple and rapid methods for the purification of stable infectious recombinant adenoviruses (rAds) remains a challenge. We report here a method for the purification of infectious adenovirus type 5 (Ad5) that involves ultracentrifugation on a cesium chloride gradient at 604,000g for 15 min at 4 deg C and tangential flow filtration. The entire procedure requires less than two hours and infectious Ad5 can be recovered at levels higher than 64% of the number of plaque-forming units (pfu) in the initial crude preparation of viruses. We have obtained titers of infectious purified Ad5 of 1.35 x 10 10 pfu/ml and a ratio of particle titer to infectious titer of seven. The method described here allows the rapid purification of rAds for studies of gene function in vivo and in vitro, as well as the rapid purification of Ad5

  6. Canine Adenovirus Type 2 Vector Generation via I-Sce1-Mediated Intracellular Genome Release

    Science.gov (United States)

    Ibanes, Sandy; Kremer, Eric J.

    2013-01-01

    When canine adenovirus type 2 (CAdV-2, or also commonly referred to as CAV-2) vectors are injected into the brain parenchyma they preferentially transduce neurons, are capable of efficient axonal transport to afferent regions, and allow transgene expression for at last >1 yr. Yet, translating these data into a user-friendly vector platform has been limited because CAV-2 vector generation is challenging. Generation of E1-deleted adenovirus vectors often requires transfection of linear DNA fragments of >30 kb containing the vector genome into an E1-transcomplementing cell line. In contrast to human adenovirus type 5 vector generation, CAV-2 vector generation is less efficient due, in part, to a reduced ability to initiate replication and poor transfectibility of canine cells with large, linear DNA fragments. To improve CAV-2 vector generation, we generated an E1-transcomplementing cell line expressing the estrogen receptor (ER) fused to I-SceI, a yeast meganuclease, and plasmids containing the I-SceI recognition sites flanking the CAV-2 vector genome. Using transfection of supercoiled plasmid and intracellular genome release via 4-OH-tamoxifen-induced nuclear translocation of I-SceI, we improved CAV-2 vector titers 1,000 fold, and in turn increased the efficacy of CAV-2 vector generation. PMID:23936483

  7. Adenovirus-mediated sphingomyelin synthase 2 increases atherosclerotic lesions in ApoE KO mice

    Directory of Open Access Journals (Sweden)

    Zhao Yarui

    2011-01-01

    Full Text Available Abstract Background Sphingomyelin synthase 2 (SMS2 contributes to de novo sphingomyelin (SM biosynthesis. Its activity is related to SM levels in the plasma and the cell membrane. In this study, we investigated the possibility of a direct relationship between SMS and atherosclerosis. Methods The Adenovirus containing SMS2 gene was given into 10-week ApoE KO C57BL/6J mice by femoral intravenous injection. In the control group, the Adenovirus containing GFP was given. To confirm this model, we took both mRNA level examination (RT-PCR and protein level examination (SMS activity assay. Result We generated recombinant adenovirus vectors containing either human SMS2 cDNA (AdV-SMS2 or GFP cDNA (AdV-GFP. On day six after intravenous infusion of 2 × 1011 particle numbers into ten-week-old apoE KO mice, AdV-SMS2 treatment significantly increased liver SMS2 mRNA levels and SMS activity (by 2.7-fold, 2.3-fold, p Conclusions Our results present direct morphological evidence for the pro-atherogenic capabilities of SMS2. SMS2 could be a potential target for treating atherosclerosis.

  8. Combination of adenovirus and cross-linked low molecular weight PEI improves efficiency of gene transduction

    International Nuclear Information System (INIS)

    Han Jianfeng; Zhao Dong; Zhong Zhirong; Zhang Zhirong; Gong Tao; Sun Xun

    2010-01-01

    Recombinant adenovirus (Ad)-mediated gene therapy is an exciting novel strategy in cancer treatment. However, poor infection efficiency with coxsackievirus and adenovirus receptor (CAR) down-regulated cancer cell lines is one of the major challenges for its practical and extensive application. As an alternative method of viral gene delivery, a non-viral carrier using cationic materials could compensate for the limitation of adenovirus. In our study, adenovectors were complexed with a new synthetic polymer PEI-DEG-bis-NPC (PDN) based on polyethylenimine (PEI), and then the properties of the vehicle were characterized by measurement of size distribution, zeta potential and transmission electron microscopy (TEM). Enhancement of gene transduction by Ad/PDN complexes was observed in both CAR-overexpressing cell lines (A549) and CAR-lacking cell lines (MDCK, CHO, LLC), as a result of facilitating binding and cell uptake of adenoviral particles by the cationic component. Ad/PDN complexes also promoted the inhibition of tumor growth in vivo and prolonged the survival time of tumor-bearing mice. These data suggest that a combination of viral and non-viral gene delivery methods may offer a new approach to successful cancer gene therapy.

  9. A rapid generation of adenovirus vector with a genetic modification in hexon protein.

    Science.gov (United States)

    Di, Bingyan; Mao, Qinwen; Zhao, Junli; Li, Xing; Wang, Dongyang; Xia, Haibin

    2012-02-10

    The generation of hexon-modified adenovirus vector has proven difficult. In this paper, we developed a novel method for rapid generation of hexon-modified adenoviral vector via one step ligation in vitro followed by quick white/blue color screening. The new system has the following features. First, eGFP expression driven by the CMV promoter in E1 region functions as a reporter to evaluate the tropism of hexon-modified adenovirus in vitro. Second, it has two unique restriction enzyme sites with sticky ends located in the hexon HVR5 region. Third, a lacZ expression cassette under the control of plac promoter is placed between the two restriction enzyme sites, which allows recombinants to be selected using blue/white screening. To prove the principle of the method, genetically modified adenoviruses were successfully produced by insertion of NGR, RGD or Tat PTD peptide into hexon HVR5. Furthermore, the transduction efficiency of the Tat PTD modified virus was shown to be a significant enhancement in A172 and CHO-K1 cells. In conclusion, the novel system makes the production of truly retargeted vectors more promising, which would be of substantial benefit for cancer gene therapy. Copyright © 2012 Elsevier B.V. All rights reserved.

  10. Canine adenovirus type 2 vector generation via I-Sce1-mediated intracellular genome release.

    Directory of Open Access Journals (Sweden)

    Sandy Ibanes

    Full Text Available When canine adenovirus type 2 (CAdV-2, or also commonly referred to as CAV-2 vectors are injected into the brain parenchyma they preferentially transduce neurons, are capable of efficient axonal transport to afferent regions, and allow transgene expression for at last >1 yr. Yet, translating these data into a user-friendly vector platform has been limited because CAV-2 vector generation is challenging. Generation of E1-deleted adenovirus vectors often requires transfection of linear DNA fragments of >30 kb containing the vector genome into an E1-transcomplementing cell line. In contrast to human adenovirus type 5 vector generation, CAV-2 vector generation is less efficient due, in part, to a reduced ability to initiate replication and poor transfectibility of canine cells with large, linear DNA fragments. To improve CAV-2 vector generation, we generated an E1-transcomplementing cell line expressing the estrogen receptor (ER fused to I-SceI, a yeast meganuclease, and plasmids containing the I-SceI recognition sites flanking the CAV-2 vector genome. Using transfection of supercoiled plasmid and intracellular genome release via 4-OH-tamoxifen-induced nuclear translocation of I-SceI, we improved CAV-2 vector titers 1,000 fold, and in turn increased the efficacy of CAV-2 vector generation.

  11. Enhanced transduction and replication of RGD-fiber modified adenovirus in primary T cells.

    Directory of Open Access Journals (Sweden)

    Sadhak Sengupta

    2011-03-01

    Full Text Available Adenoviruses are often used as vehicles to mediate gene delivery for therapeutic purposes, but their research scope in hematological cells remains limited due to a narrow choice of host cells that express the adenoviral receptor (CAR. T cells, which are attractive targets for gene therapy of numerous diseases, remain resistant to adenoviral infection because of the absence of CAR expression. Here, we demonstrate that this resistance can be overcome when murine or human T cells are transduced with an adenovirus incorporating the RGD-fiber modification (Ad-RGD.A luciferase-expressing replication-deficient Ad-RGD infected 3-fold higher number of activated primary T cells than an adenovirus lacking the RGD-fiber modification in vitro. Infection with replication-competent Ad-RGD virus also caused increased cell cycling, higher E1A copy number and enriched hexon antigen expression in both human and murine T cells. Transduction with oncolytic Ad-RGD also resulted in higher titers of progeny virus and enhanced the killing of T cells. In vivo, 35-45% of splenic T cells were transduced by Ad-RGD.Collectively, our results prove that a fiber modified Ad-RGD successfully transduces and replicates in primary T cells of both murine and human origin.

  12. [Outbreak of follicular conjunctivitis caused by adenovirus in a geriatric centre].

    Science.gov (United States)

    Artieda, Juncal; Montes, Milagrosa; Vicente, Diego; Martínez, Consuelo; Piñeiro, Luis; Mendiola, Josune

    2010-12-01

    Adenovirus serotype 4a is a respiratory virus that occasionally causes conjunctivitis. This paper describes an outbreak of follicular conjunctivitis that occurred in a geriatric centre. Outbreak description and epidemiological research through a survey. For the microbiological study conjunctival swabs were collected using viral and bacterial transport media. Adenovirus was detected by real-time polymerase chain reaction. The serotype was determined by sequencing of a fragment of the hexon and E1 genes. In autumn 2008 an outbreak of follicular conjunctivitis caused by adenovirus serotype 4a was detected. Twenty three percent 23% (69/300) of residents and 5% (9/180) of workers in a geriatric centre in Gipuzkoa were affected. The clinical symptoms were of prolonged duration (11±5 days). The temporal association of the cases suggested transmission from person to person. The sanitary measures established (asepsis and frequent hand washing, cleaning and disinfection of objects and surfaces) were effective, interrupting the transmission of the disease within a short period of time. Rapid detection, identification of the causative agent and implementing appropriate control measures can significantly reduce the impact on both health and economic costs of these outbreaks. Copyright © 2009 Elsevier España, S.L. All rights reserved.

  13. Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum).

    Science.gov (United States)

    Ogawa, Hirohito; Kajihara, Masahiro; Nao, Naganori; Shigeno, Asako; Fujikura, Daisuke; Hang'ombe, Bernard M; Mweene, Aaron S; Mutemwa, Alisheke; Squarre, David; Yamada, Masao; Higashi, Hideaki; Sawa, Hirofumi; Takada, Ayato

    2017-12-04

    Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats ( Eidolon helvum ) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus . The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E , F and G , from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name " Bat mastadenovirus H ". Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.

  14. Viral capsid is a pathogen-associated molecular pattern in adenovirus keratitis.

    Directory of Open Access Journals (Sweden)

    Ashish V Chintakuntlawar

    2010-04-01

    Full Text Available Human adenovirus (HAdV infection of the human eye, in particular serotypes 8, 19 and 37, induces the formation of corneal subepithelial leukocytic infiltrates. Using a unique mouse model of adenovirus keratitis, we studied the role of various virus-associated molecular patterns in subsequent innate immune responses of resident corneal cells to HAdV-37 infection. We found that neither viral DNA, viral gene expression, or viral replication was necessary for the development of keratitis. In contrast, empty viral capsid induced keratitis and a chemokine profile similar to intact virus. Transfected viral DNA did not induce leukocyte infiltration despite CCL2 expression similar to levels in virus infected corneas. Mice without toll-like receptor 9 (Tlr9 signaling developed clinical keratitis upon HAdV-37 infection similar to wild type mice, although the absolute numbers of activated monocytes in the cornea were less in Tlr9(-/- mice. Virus induced leukocytic infiltrates and chemokine expression in mouse cornea could be blocked by treatment with a peptide containing arginine glycine aspartic acid (RGD. These results demonstrate that adenovirus infection of the cornea induces chemokine expression and subsequent infiltration by leukocytes principally through RGD contact between viral capsid and the host cell, possibly through direct interaction between the viral capsid penton base and host cell integrins.

  15. Antiviral T Cells for Adenovirus in the Pretransplant Period: A Bridge Therapy for Severe Combined Immunodeficiency.

    Science.gov (United States)

    Miller, Holly K; Hanley, Patrick J; Lang, Haili; Lazarski, Christopher A; Chorvinsky, Elizabeth A; McCormack, Sarah; Roesch, Lauren; Albihani, Shuroug; Dean, Marcus; Hoq, Fahmida; Adams, Roberta H; Bollard, Catherine M; Keller, Michael D

    2018-05-09

    Viral infections can be life threatening in patients with severe combined immunodeficiency (SCID) and other forms of profound primary immunodeficiency disorders both before and after hematopoietic stem cell transplantation (HSCT). Adoptive immunotherapy with virus-specific T cells (VSTs) has been utilized in many patients in the setting of HSCT, but has very rarely been attempted for treatment of viral infections before HSCT. Here we describe the use of VSTs in an infant with RAG1 SCID who had developed disseminated adenovirus which failed to improve on cidofovir. Adenovirus cleared following 2 doses of VSTs and marrow infusion from a matched unrelated donor, without incidence of graft versus host disease. T cell receptor-b sequencing demonstrated expansion of adenovirus-specific T cell fraction of the VSTs, suggesting that infusion facilitated viral clearance. This report suggests that VSTs are likely safe in the pre-HSCT period, and may be a useful bridge therapy for infants with SCID and persistent viral infections. Copyright © 2018 The American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved.

  16. Inhibitory effect of recombinant adenovirus carrying immunocaspase-3 on hepatocellular carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Li, Xiaohua [State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi' an 710032 (China); Fan, Rui [State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi' an 710032 (China); Zou, Xue [State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi' an 710032 (China); Gao, Lin [State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi' an 710032 (China); Jin, Haifeng [State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi' an 710032 (China); Du, Rui [State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi' an 710032 (China); Xia, Lin [State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi' an 710032 (China); Fan, Daiming [State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi' an 710032 (China)

    2007-06-29

    Previously, Srinivasula devised a contiguous molecule (C-cp-3 or immunocaspase-3) containing the small and large subunits similar to that in the active form of caspas-3 and found C-cp-3 had similar cleavage activity to the active form of caspase-3. To search for a new clinical application of C-cp-3 to treat hepatocellular carcinoma, recombinant adenoviruses carrying the C-cp-3 and a-fetoprotein (AFP) promoter (Ad-rAFP-C-cp-3) were constructed through a bacterial homologous recombinant system. The efficiency of adenovirus-mediated gene transfer and the inhibitory effect of Ad-rAFP-C-cp-3 on the proliferation of hepatocarcinoma cells were determined by X-gal stain and MTT assay, respectively. The tumorigenicity of hepatocarcinoma cells transfected by Ad-rAFP-C-cp-3 and the antitumor effect of Ad-rAFP-C-cp-3 on transplanted tumor in nude mice were detected in vivo. The results suggested that Ad-rAFP-C-cp-3 can inhibit specifically proliferation of AFP-producing human hepatocarcinoma cells in vitro and in vivo and adenovirus-mediated C-cp-3 transfer could be used as a new method to treat human hepatocarcinoma.

  17. Inhibitory effect of recombinant adenovirus carrying immunocaspase-3 on hepatocellular carcinoma

    International Nuclear Information System (INIS)

    Li, Xiaohua; Fan, Rui; Zou, Xue; Gao, Lin; Jin, Haifeng; Du, Rui; Xia, Lin; Fan, Daiming

    2007-01-01

    Previously, Srinivasula devised a contiguous molecule (C-cp-3 or immunocaspase-3) containing the small and large subunits similar to that in the active form of caspas-3 and found C-cp-3 had similar cleavage activity to the active form of caspase-3. To search for a new clinical application of C-cp-3 to treat hepatocellular carcinoma, recombinant adenoviruses carrying the C-cp-3 and a-fetoprotein (AFP) promoter (Ad-rAFP-C-cp-3) were constructed through a bacterial homologous recombinant system. The efficiency of adenovirus-mediated gene transfer and the inhibitory effect of Ad-rAFP-C-cp-3 on the proliferation of hepatocarcinoma cells were determined by X-gal stain and MTT assay, respectively. The tumorigenicity of hepatocarcinoma cells transfected by Ad-rAFP-C-cp-3 and the antitumor effect of Ad-rAFP-C-cp-3 on transplanted tumor in nude mice were detected in vivo. The results suggested that Ad-rAFP-C-cp-3 can inhibit specifically proliferation of AFP-producing human hepatocarcinoma cells in vitro and in vivo and adenovirus-mediated C-cp-3 transfer could be used as a new method to treat human hepatocarcinoma

  18. Nucleotide sequence determination of the region in adenovirus 5 DNA involved in cell transformation

    International Nuclear Information System (INIS)

    Maat, J.

    1978-01-01

    A description is given of investigations into the primary structure of the transforming region of adenovirus type 5 DNA. The phenomenon of cell transformation is discussed in general terms and the principles of a number of fairly recent techniques, which have been in use for DNA sequence determination since 1975 are dealt with. A few of the author's own techniques are described which deal both with nucleotide sequence analysis and with the determination of DNA cleavage sites of restriction endonucleases. The results are given of the mapping of cleavage sites in the HpaI-E fragment of adenovirus DNA of HpaII, HaeIII, AluI, HinfI and TaqI and of the determination of the nucleotide sequence in the transforming region of adenovirus type 5 DNA. The results of the sequence determination of the Ad5 HindIII-G fragment are discussed in relation with the investigation on the transforming proteins isolated from in vitro and in vivo synthesizing systems. Labelling procedures of DNA are described including the exonuclease III/DNA polymerase 1 method and TA polynucleotide kinase labelling of DNA fragments. (Auth.)

  19. Sequence typing of adenovirus from samples from hematological stem cell transplant recipients.

    Science.gov (United States)

    Al Qurashi, Yasir Mohammed A; Guiver, Malcolm; Cooper, Robert J

    2011-11-01

    Adenovirus infections are usually mild or even asymptomatic, but infections with the virus are being recognized increasingly as a major cause of mortality and morbidity in the immunocompromised, particularly hematological stem cell transplant patients where infections can be life threatening and mortality may reach 60%. Typing by sequencing the HVR7 region of the hexon was established and validated using 60 isolates of different serotypes from the six of the seven species which had been typed previously by serum neutralization. Analysis of nucleotide sequences was used to type 227 samples from 41 hematological stem cell transplant recipients. Types from six species were detected but species C types were detected in 51.4% and species A in 34.3% of patients. Seven patients were infected with different adenovirus types sequentially and a further six patients had evidence of simultaneous multiple infections. Many of the sequences had several differences from the prototype strains which will allow tracing of outbreaks and provide evidence for cross-infection in a hospital setting. In this study, the phylogenetic analysis of adenovirus sequences from hematological stem cell transplant patients' samples showed evidence of two possible cross-infection incidents involving three and five patients, respectively. Copyright © 2011 Wiley-Liss, Inc.

  20. Retrovirus endógenos humanos: Significado biológico e implicaciones evolutivas

    Directory of Open Access Journals (Sweden)

    Sentís, Carlos

    2002-05-01

    Full Text Available El genoma humano contiene un importante número de retrovirus endógenos (HERVs, es decir, secuencias derivadas de pasadas infecciones retrovirales insertadas de forma permanente; y secuencias similares se pueden observar en prácticamente todos los organismos eucariontes. Muchos de estos HERVs se transcriben y traducen en condiciones fisiológicas normales, llegando a formar partículas virales completas, y participando en procesos tan complejos como la placentación. Por su capacidad de retrotransposición y recombinación entre ellos son una fuente importante de remodelación genómica y, junto con otros retroelementos, participan en la generación de retrogenes y retropseudogenes, que suponen un sustrato de variabilidad informacional fundamental para la aparición de nuevas estructuras y funciones. Puesto que su actividad responde también a las condiciones ambientales, los cambios genómicos generados por ellos no son graduales, sino que aparecen en oleadas, de modo que se puede producir una variedad fenotípica muy extensa en momentos evolutivos concretos, coincidiendo con situaciones ambientales críticas. La consideración de los HERVs como parte integral y consustancial de nuestro genoma obliga a replantearse la utilización de vectores retrovirales en protocolos de terapia génica, así como la utilización de órganos animales -con sus propios retrovirus endógenos- para xenotrasplantes.

  1. Genes de susceptibilidad/ resistencia a Flavivirus, implicaciones en la severidad de la infección

    Directory of Open Access Journals (Sweden)

    Jeanette Prada-Arismendy

    2006-07-01

    Full Text Available Las infecciones transmitidas por Flavivirus se encuentran entre las enfermedades transmisibles con mayor incidencia en el mundo. La mayoría de ellas se manifiestan clínicamente como un síndrome febril que puede estar o no acompañado de diversos síntomas. La severidad de estas infecciones es variable con casos asintomáticos y otros que pueden llegar a ser letales, La razón de esta variabilidad en la presentación clínica, se desconoce en humanos. En ratones se han identificado cepas susceptibles y cepas resistentes a la infección por algunos Flavivirus. Por clonación posicional se mapeó el gen responsable de la resistencia a virus West Nile en el cromosoma 5 de ratón y se identificó como oligoadenilato sintetasa 1b (Oas1 b. Este gen codifica una proteína que sintetiza oligómeros de adenina que activan la RNasaL, que a su vez degrada los RNAs virales. Células provenientes de ratones resistentes a la infección por Flavivirus producen menor cantidad de virus que su contraparte susceptible, Recientemente en humanos, se identificó un polimorfismo asociado con susceptibilidad a infección por virus West Nile en el gen de OasL. Sin embargo, el mecanismo bioquímico y molecular exacto por el cual se produce la susceptibilidad no ha sido completamente dilucidado, Este conocimiento permitiría aclarar aspectos de la fisiopatología de estas enfermedades y enfocar la terapéutica desde un punto de vista más específico.

  2. El desarrollo turístico en la isla de Lanzarote: las implicaciones económicas y ecológicas

    Directory of Open Access Journals (Sweden)

    Alejandro González Morales

    2013-11-01

    Full Text Available El presente trabajo versa sobre las implicaciones medioambientales del desarrollo turístico en la isla de Lanzarote. En este sentido hemos comprobado como su crecimiento afecta al resto de las actividades y ha terciarizado de forma considerable la economía insular.The present article is on turist's develop of Lanzarote Island (Lanary Island. The agriculture is going down, whole than the tourist is over. The economy of island is very third.

  3. Implicaciones de la inteligencia emocional en los constructos resiliencia y satisfacción vital

    OpenAIRE

    Rubio Martín, María Jesús

    2016-01-01

    Esta investigación describe y analiza los constructos Inteligencia Emocional, Resiliencia y Satisfacción con la vida en una muestra de 2.066 estudiantes de la universidad de Castilla-La Mancha de diferentes grados académicos. La vida universitaria es un contexto que puede ser una fuente de bienestar y en ocasiones de estrés debido a que los estudiantes se enfrentan a múltiples cambios siendo a la vez una etapa de tránsito. El Espacio Europeo de Educación Superior aboga por una formación centr...

  4. El dolor en la enfermedad de Parkinson. Implicaciones autonómicas y afectivas

    OpenAIRE

    Camacho Conde, José

    2016-01-01

    Introducción y objetivo: La enfermedad de Parkinson (EP) es una enfermedad neurodegenerativa, la segunda con mayor prevalencia, después de la enfermedad de Alzheimer (EA). La enfermedad presenta tanto síntomas motores como “no motores”, entre los que se encuentra disfunción autonómica, dolor, deterioro cognitivo, ansiedad, depresión, entre otros. El dolor en la EP, a pesar de su frecuencia, sigue siendo un síntoma infravalorado, infradiagnosticado e infratratado. Nuestro objetivo principal es...

  5. Libre comercio en norteamerica. Algunas explicaciones e implicaciones para México

    OpenAIRE

    Carlos GONZÁLEZ MARTÍNEZ

    2009-01-01

    RESUMEN: El autor exmina las oportunidades y retos que puede encontrar México en el tratado de Libre Comercio, firmado entre México, Estados Unidos y Canadá. Considera esta integración económica como parte de un proceso más amplio de reforma del Estado y replanteamiento de los vínculos económicos con el exterior.ABSTRACT: The author analizes the opportunities and challenges that the Free Trade Agreement signed by México, The United States and Canada represents for Mexico. This economic integr...

  6. El conductismo y algunas implicaciones de lo que significa ser conductista hoy

    OpenAIRE

    Camilo Hurtado

    2006-01-01

    Se discuten varios aspectos relacionados con el conductismo, sus múltiples versiones, una definición amplia de éste y a quienes se les puede denominar actualmente conductistas. Además, a partir de una anécdota se analiza, en términos de barreras epistemológicas, el rechazo a esta tradición de pensamiento y se discute como dicho rechazo está enraizado y mantenido en una serie de malentendidos y en las contradicciones que existen entre los principios del conductismo genérico y los de la llamad...

  7. Implicaciones del protocolo de Madrid en el derecho marcario de la comunidad andina de naciones

    Directory of Open Access Journals (Sweden)

    Edwin Jesith Bernal Ramírez

    2013-11-01

    Full Text Available El Protocolo de Madrid de 27 de junio de 1989 es una herramienta útil para el registro internacional de marcas, pues es evidente que los países miembros del Protocolo tienen ventajas comparativas respecto a los otros que no son parte, esto en relación con el mejoramiento de la protección internacional de sus marcas. El artículo académico muestra de manera somera el procedimiento, las ventajas y desventajas del Protocolo de Madrid y su respectivo impacto en la Comunidad Andina de Naciones.

  8. Turismo y Dengue en Cozumel: una aproximación desde sus implicaciones.

    Directory of Open Access Journals (Sweden)

    Alejandro Palafox Muñoz

    2013-01-01

    Full Text Available El turismo es un importante sector estratégico para el crecimiento económico de Quintana Roo, ya que el 75% de su población está involucrada directa o indirectamente con la actividad. Asimismo, en Cozumel, la vida económica se desarrolla alrededor del turismo de cruceros y buceo por la calidad de sus recursos naturales submarinos. Sin embargo, la dependencia económica hacia un sector de la economía, provoca alteraciones en la estructura socioeconómica de las localidades, al verse afectadas por alguna contingencia de tipo ambiental o sanitaria; ejemplo de ello es el brote del virus de la influenza AH1N1 que afectó sensiblemente al sector turístico. En este sentido, el turismo y la salud son dos conceptos estrechamente relacionados, dado que ambos inciden directamente en la economía de los países y son parte de la política social (Chávez, 1994. En la zona del Caribe existe la amenaza de contraer dengue, enfermedad transmitida por el mosquito Aedes aegypti (vector; esta afección viral genera daños cuantiosos en la economía, en las actividades productivas y en el turismo (Zúñiga et al., 2009. Con base en lo anterior, el presente artículo pretende revelar el impacto del dengue en el sector turístico de Cozumel durante el año 2011. La metodología que se aplicó para este estudio está basada en la utilización de métodos cuantitativos (cuestionario y métodos cualitativos (observación directa. La aplicación del instrumento de campo se realizó mediante un muestreo de tipo estratificado con base en las Zonas de Atención Prioritaria (ZAP’s de la localidad (Frausto y Rojas, 2005. Con la información obtenida del trabajo de campo, mediante la aplicación del instrumento a las diferentes colonias, algunos resultados permitirán identificar que la población afectada forma parte del sector turístico, así como también cuáles fueron los impactos de esta enfermedad en el ámbito social y

  9. La época del imperialismo y sus implicaciones en la salud pública

    OpenAIRE

    Estrada M, John H

    2008-01-01

    Este artículo estudia el denominado gran siglo XIX o largo siglo XIX, que comprende todo este siglo más las décadas que antecedieron y acompañaron a la primera guerra mundial y que se corresponde con la consolidación del imperialismo. Desde la perspectiva de la salud pública, es importante por haberse producido la transición de la higiene a la salud pública y que ya en 1880 caracteriza el advenimiento de la teoría bacteriológica y su influencia en las medidas de sanidad estatal imperantes. La...

  10. Conversando con Oriol Bohigas

    OpenAIRE

    Redondo Domínguez, Ernesto; Moya Sala, Joaquim

    2015-01-01

    [EN] Interview with Oriol Bohigas [ES] Entrevista con Oriol Bohigas Redondo Domínguez, E.; Moya Sala, J. (2015). Conversando con… Oriol Bohigas. EGA. Revista de Expresión Gráfica Arquitectónica. 20(26):22-35. doi:10.4995/ega.2015.4061 22 35 20 26

  11. DR Con o:

    African Journals Online (AJOL)

    which could fall under the Ugandan influence. The con-. flict in the ..... The Congolese people and international community within SADC, the AU ..... ments and make peace among themselves. However, one ... friends overnight.There is a great ...

  12. Adenovirus-dependent changes in cell membrane permeability: role of Na/sup +/, K/sup +/-ATPase

    Energy Technology Data Exchange (ETDEWEB)

    Seth, P.; Pastan, I.; Willingham, M.C.

    1987-03-01

    Adenovirus-dependent release of choline phosphate from KB cells at pH 6.0 was partially blocked by ouabain. In K/sup +/-containing medium, maximum inhibition of release was obtained by 10/sup -5/ M ouabain and half-maximal inhibition was achieved by about 0.5 x 10/sup -6/ M ouabain. Ouabain did not block either the binding or the uptake of adenovirus by KB cells. Without K/sup +/, about 25% of cell-associated choline phosphate was released by adenovirus, whereas with 1 mM K/sup +/ about 50% was released. This activation by K/sup +/ was blocked by 0.1 mM ouabain. HeLa cells behaved like KB cells, but a mutant of HeLa cells resistant to ouabain (D98-OR) released much lower amounts of choline phosphate in response to human adenovirus type 2 (Ad2). Wild-type D98-OR cells bound nearly the same amount of adenovirus as did normal HeLa cells. Ad2 also increased the activity of Na/sup +/, K/sup +/-ATPase in KB cells, with maximum activation at 50..mu..g of Ad2 per ml. In D98-OR cells, Ad2 failed to activate Na/sup +/, K/sup +/, ATPase activity. Ad2-dependent lysis of endocytic vesicles (receptosomes) was assayed by measuring Ad2-dependent enhancement of epidermal growth factor-Pseudomonas exotoxin toxicity. This action of adenovirus was increased when K/sup +/ was present in the medium. Under the conditions used, K/sup +/ had no effect on the amount of Ad2 or epidermal growth factor taken up by the cells. On the basis of these results, it is suggested that Ad2-dependent cellular efflux of choline phosphate and adenovirus-dependent lysis of receptosomes may require Na/sup +/, K/sup +/-ATPase activity.

  13. fertilizada con diferentes abonos

    Directory of Open Access Journals (Sweden)

    Jorge Alberto Elizondo-Salazar

    2007-01-01

    Full Text Available Producción y calidad de la biomasa de morera (Morus alba fertilizada con diferentes abonos. Se llevó a cabo un experimento en la Estación Experimental “Alfredo Volio Mata” de la Universidad de Costa Rica con el fi n de evaluar la aplicación de 150 kg de N/ha/año proveniente de dos abonos orgánicos: lombriabono y compostaje; y de un fertilizante químico, sobre la producción y calidad de la biomasa de morera. El periodo experimental comprendió un ciclo de 12 meses, iniciando en julio del 2003 y fi nalizando en julio del 2004. Se utilizó una plantación de morera de 12 años de establecida con una densidad de siembra de 27.777 plantas/ ha. Se empleó un diseño de bloques completos al azar con cuatro tratamientos: dos abonos orgánicos, nitrato de amonio (33,5% N y un control. Las plantas se podaron a 0,6 m sobre el nivel del suelo al inicio del ensayo. Durante el periodo experimental, las plantas fueron podadas consecutivamente cada 90 días. Las hojas y los tallos fueron separados y analizados para determinar el contenido de materia seca y proteína cruda. La producción de materia seca fue 23% superior y el contenido de proteína cruda fue signifi cativamente mayor con el nitrógeno químico, mientras que el contenido de materia seca fue menor. No se encontraron diferencias signifi cativas entre el tratamiento control y los tratamientos orgánicos.

  14. Enhanced protection against Ebola virus mediated by an improved adenovirus-based vaccine.

    Science.gov (United States)

    Richardson, Jason S; Yao, Michel K; Tran, Kaylie N; Croyle, Maria A; Strong, James E; Feldmann, Heinz; Kobinger, Gary P

    2009-01-01

    The Ebola virus is transmitted by direct contact with bodily fluids of infected individuals, eliciting death rates as high as 90% among infected humans. Currently, replication defective adenovirus-based Ebola vaccine is being studied in a phase I clinical trial. Another Ebola vaccine, based on an attenuated vesicular stomatitis virus has shown efficacy in post-exposure treatment of nonhuman primates to Ebola infection. In this report, we modified the common recombinant adenovirus serotype 5-based Ebola vaccine expressing the wild-type ZEBOV glycoprotein sequence from a CMV promoter (Ad-CMVZGP). The immune response elicited by this improved expression cassette vector (Ad-CAGoptZGP) and its ability to afford protection against lethal ZEBOV challenge in mice was compared to the standard Ad-CMVZGP vector. Ad-CMVZGP was previously shown to protect mice, guinea pigs and nonhuman primates from an otherwise lethal challenge of Zaire ebolavirus. The antigenic expression cassette of this vector was improved through codon optimization, inclusion of a consensus Kozak sequence and reconfiguration of a CAG promoter (Ad-CAGoptZGP). Expression of GP from Ad-CAGoptZGP was substantially higher than from Ad-CMVZGP. Ad-CAGoptZGP significantly improved T and B cell responses at doses 10 to 100-fold lower than that needed with Ad-CMVZGP. Additionally, Ad-CAGoptZGP afforded full protections in mice against lethal challenge at a dose 100 times lower than the dose required for Ad-CMVZGP. Finally, Ad-CAGoptZGP induced full protection to mice when given 30 minutes post-challenge. We describe an improved adenovirus-based Ebola vaccine capable of affording post-exposure protection against lethal challenge in mice. The molecular modifications of the new improved vaccine also translated in the induction of significantly enhanced immune responses and complete protection at a dose 100 times lower than with the previous generation adenovirus-based Ebola vaccine. Understanding and improving the

  15. Enhanced protection against Ebola virus mediated by an improved adenovirus-based vaccine.

    Directory of Open Access Journals (Sweden)

    Jason S Richardson

    Full Text Available BACKGROUND: The Ebola virus is transmitted by direct contact with bodily fluids of infected individuals, eliciting death rates as high as 90% among infected humans. Currently, replication defective adenovirus-based Ebola vaccine is being studied in a phase I clinical trial. Another Ebola vaccine, based on an attenuated vesicular stomatitis virus has shown efficacy in post-exposure treatment of nonhuman primates to Ebola infection. In this report, we modified the common recombinant adenovirus serotype 5-based Ebola vaccine expressing the wild-type ZEBOV glycoprotein sequence from a CMV promoter (Ad-CMVZGP. The immune response elicited by this improved expression cassette vector (Ad-CAGoptZGP and its ability to afford protection against lethal ZEBOV challenge in mice was compared to the standard Ad-CMVZGP vector. METHODOLOGY/PRINCIPAL FINDINGS: Ad-CMVZGP was previously shown to protect mice, guinea pigs and nonhuman primates from an otherwise lethal challenge of Zaire ebolavirus. The antigenic expression cassette of this vector was improved through codon optimization, inclusion of a consensus Kozak sequence and reconfiguration of a CAG promoter (Ad-CAGoptZGP. Expression of GP from Ad-CAGoptZGP was substantially higher than from Ad-CMVZGP. Ad-CAGoptZGP significantly improved T and B cell responses at doses 10 to 100-fold lower than that needed with Ad-CMVZGP. Additionally, Ad-CAGoptZGP afforded full protections in mice against lethal challenge at a dose 100 times lower than the dose required for Ad-CMVZGP. Finally, Ad-CAGoptZGP induced full protection to mice when given 30 minutes post-challenge. CONCLUSIONS/SIGNIFICANCE: We describe an improved adenovirus-based Ebola vaccine capable of affording post-exposure protection against lethal challenge in mice. The molecular modifications of the new improved vaccine also translated in the induction of significantly enhanced immune responses and complete protection at a dose 100 times lower than with the

  16. Prevalence, quantification and typing of adenoviruses detected in river and treated drinking water in South Africa.

    Science.gov (United States)

    van Heerden, J; Ehlers, M M; Heim, A; Grabow, W O K

    2005-01-01

    Human adenoviruses (HAds), of which there are 51 serotypes, are associated with gastrointestinal, respiratory, urinary tract and eye infections. The importance of water in the transmission of HAds and the potential health risks constituted by HAds in these environments are widely recognized. Adenoviruses have not previously been quantified in river and treated drinking water samples. In this study, HAds in river water and treated drinking water sources in South Africa were detected, quantified and typed. Adenoviruses were recovered from the water samples using a glass wool adsorption-elution method followed by polyethylene glycol/NaCl precipitation for secondary concentration. The sensitivity and specificity of two nested PCR methods were compared for detection of HAds in the water samples. Over a 1-year period (June 2002 to July 2003), HAds were detected in 5.32% (10/188) of the treated drinking water and 22.22% (10/45) of river water samples using the conventional nested PCR method. The HAds detected in the water samples were quantified using a real-time PCR method. The original treated drinking water and river water samples had an estimate of less than one copy per litre of HAd DNA present. The hexon-PCR products used for typing HAds were directly sequenced or cloned into plasmids before sequencing. In treated drinking water samples, species D HAds predominated. In addition, adenovirus serotypes 2, 40 and 41 were each detected in three different treated drinking water samples. Most (70%) of the HAds detected in river water samples analysed were enteric HAds (serotypes 40 and 41). One HAd serotype 2 and two species D HAds were detected in the river water. Adenoviruses detected in river and treated drinking water samples were successfully quantified and typed. The detection of HAds in drinking water supplies treated and disinfected by internationally recommended methods, and which conform to quality limits for indicator bacteria, warrants an investigation of the

  17. Crystallization of the C-terminal head domain of the avian adenovirus CELO long fibre

    Energy Technology Data Exchange (ETDEWEB)

    Guardado Calvo, Pablo [Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus Sur, E-15782 Santiago de Compostela (Spain); Llamas-Saiz, Antonio L. [Unidad de Difracción de Rayos X, Laboratorio Integral de Dinámica y Estructura de Biomoléculas José R. Carracido, Edificio CACTUS, Universidad de Santiago de Compostela, Campus Sur, E-15782 Santiago de Compostela (Spain); Langlois, Patrick [Agence Francaise de Securité Sanitaire des Aliments, Unité Génétique Virale et Biosecurité, Site Les Croix, BP 53, F-22440 Ploufragan (France); Raaij, Mark J. van, E-mail: vanraaij@usc.es [Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus Sur, E-15782 Santiago de Compostela (Spain); Unidad de Difracción de Rayos X, Laboratorio Integral de Dinámica y Estructura de Biomoléculas José R. Carracido, Edificio CACTUS, Universidad de Santiago de Compostela, Campus Sur, E-15782 Santiago de Compostela (Spain)

    2006-05-01

    Avian adenovirus long-fibre head trimers were expressed, purified and crystallized. The crystals belong to space group C2 (unit-cell parameters a = 216.5, b = 59.2, c = 57.5 Å, β = 101.3°). A complete highly redundant data set was collected to 2.2 Å resolution at 100 K using a rotating-anode X-ray source. Avian adenovirus CELO contains two different fibres: fibre 1, the long fibre, and fibre 2, the short fibre. The short fibre is responsible for binding to an unknown avian receptor and is essential for infection of birds. The long fibre is not essential, but is known to bind the coxsackievirus and adenovirus receptor protein. Both trimeric fibres are attached to the same penton base, of which each icosahedral virus contains 12 copies. The short fibre extends straight outwards, while the long fibre emerges at an angle. The carboxy-terminal amino acids 579–793 of the avian adenovirus long fibre have been expressed with an amino-terminal hexahistidine tag and the expressed trimeric protein has been purified by nickel-affinity chromatography and crystallized. Crystals were grown at low pH using PEG 10 000 as precipitant and belonged to space group C2. The crystals diffracted rotating-anode Cu Kα radiation to at least 1.9 Å resolution and a complete data set was collected from a single crystal to 2.2 Å resolution. Unit-cell parameters were a = 216.5, b = 59.2, c = 57.5 Å, β = 101.3°, suggesting one trimer per asymmetric unit and a solvent content of 46%. The long fibre head does not have significant sequence homology to any other protein of known structure and molecular-replacement attempts with known fibre-head structures were unsuccessful. However, a map calculated using SIRAS phasing shows a clear trimer with a shape similar to known adenovirus fibre-head structures. Structure solution is in progress.

  18. Modelos numéricos para la simulación de incendios en túneles con ventilación semi transversal y trampillas de extracción

    OpenAIRE

    Retana Zúñiga, Mª Paloma; Fraile de Lerma, Alberto; Rey Llorente, Ignacio del; Alarcón Álvarez, Enrique

    1999-01-01

    El incremento experimentado por la construcción de grandes túneles ha provocado un renovado interés por el estudio de las diferentes situaciones de accidente que se pueden producir durante su vida de servicio. Entre ellos uno de los más complicados y peor conocidos es el caso de un accidente con fuego en el interior del túnel. Mediante el uso de un método numérico de volúmenes finitos este articulo presenta un estudio sobre las implicaciones del uso de trampillas de extracción de humos así co...

  19. La atmósfera psíquica y los vínculos significativos de madres adolescentes gestantes y lactantes de bajo estrato socio-económico. Implicaciones sobre el desarrollo psíquico

    Directory of Open Access Journals (Sweden)

    Maggi Gutiérrez

    2007-05-01

    Full Text Available En el artículo se presentan los resultados parciales de un proyecto de investigación-intervención que buscó caracterizar la atmósfera psíquica y los vínculos significativos de madres adolescentes gestantes y lactantes, así como analizar sus implicaciones sobre el desarrollo psíquico, tanto de ellas como de sus hijos e hijas. La intervención consistió en un acompañamiento psicoterapéutico grupal de enfoque psicoanalítico, realizado durante 10 a 15 sesiones a 15 grupos con un promedio de 6 madres, en los cuales participaron 97 adolescentes entre 14 a 19 años de la ciudad de Bogotá y de estrato socioeconómico bajo (1 y 2. Durante las sesiones, un observador registraba el discurso de las participantes y el del terapeuta, para posteriormente elaborar un protocolo detallado de ambos. Los protocolos fueron sometidos a un análisis hermenéutico sucesivo que arrojó inicialmente una categorización descriptiva, y sus tendencias predominantes fueron posteriormente sometidas a una interpretación teórica desde el referente psicoanalítico. Los resultados indican que durante la gestación, el parto y la lactancia, si bien en algunos casos se encontraron experiencias satisfactorias y estados emocionales que favorecían el vínculo madre-hijo, las características predominantes de la atmósfera psíquica de las adolescentes y de sus vínculos significativos, no favorecen su desarrollo psíquico, como tampoco el del bebé o la bebé.

  20. La atmósfera psíquica y los vínculos significativos de madres adolescentes gestantes y lactantes de bajo estrato socio-económico. Implicaciones sobre el desarrollo psíquico

    Directory of Open Access Journals (Sweden)

    Maggui Gutiérrez

    2007-01-01

    Full Text Available En el artículo se presentan los resultados parciales de un proyecto de investigación-intervención que buscó caracterizar la atmósfera psíquica y los vínculos significativos de madres adolescentes gestantes y lactantes, así como analizar sus implicaciones sobre el desarrollo psíquico, tanto de ellas como de sus hijos e hijas. La intervención consistió en un acompañamiento psicoterapéutico grupal de enfoque psicoanalítico, realizado durante 10 a 15 sesiones a 15 grupos con un promedio de 6 madres, en los cuales participaron 97 adolescentes entre 14 a 19 años de la ciudad de Bogotá y de estrato socioeconómico bajo (1 y 2. Durante las sesiones, un observador registraba el discurso de las participantes y el del terapeuta, para posteriormente elaborar un protocolo detallado de ambos. Los protocolos fueron sometidos a un análisis hermenéutico sucesivo que arrojó inicialmente una categorización descriptiva, y sus tendencias predominantes fueron posteriormente sometidas a una interpretación teórica desde el referente psicoanalítico. Los resultados indican que durante la gestación, el parto y la lactancia, si bien en algunos casos se encontraron experiencias satisfactorias y estados emocionales que favorecían el vínculo madre-hijo, las características predominantes de la atmósfera psíquica de las adolescentes y de sus vínculos significativos, no favorecen su desarrollo psíquico, como tampoco el del bebé o la bebé.

  1. Identificación y establecimiento de las características motivacionales y actitudinales de los estudiantes con rendimiento académico menor de lo esperado según su capacidad (underachievement

    Directory of Open Access Journals (Sweden)

    Juan Luis Castejón

    2016-12-01

    Full Text Available El término underachievement hace referencia a la discrepancia entre la capacidad potencial y el rendimiento académico observado en el alumnado. El objetivo principal de este trabajo es la identificación del alumnado con rendimiento académico menor de lo esperado con base en su capacidad, así como establecer los factores diferenciales que les caracterizan en comparación con el alumnado con rendimiento acorde con su capacidad. Por una parte, se trata de profundizar en la definición conceptual y operativa de lo que se entiende por alumnado con capacidad mayor a su rendimiento académico, a la vez que se comparan los resultados del empleo de diferentes procedimientos de identificación. Por otra, se trata de identificar los factores motivacionales y actitudinales que caracterizan a este alumnado y lo diferencian del alumnado con rendimiento mayor del esperado y al alumnado con rendimiento acorde a lo esperado. A partir del conocimiento de estos perfiles y sus características diferenciales se pueden establecer implicaciones educativas precisas para el alumnado, los padres y el profesorado, con el objetivo final de revertir su menor rendimiento académico.

  2. Implicaciones comerciales de la Diplomacia Petrolera China en el Amazonas ecuatoriano. 2007 - 2013

    OpenAIRE

    Vargas Ariza, María Alejandra

    2014-01-01

    El presente Estudio de Caso tiene como objetivo analizar en qué medida las dinámicas comerciales de la Diplomacia Petrolera China han convertido a Ecuador en un socio estratégico para la RPCh. El petróleo como fuente de energía es primordial para llevar a cabo los procesos de industrialización y mantener el crecimiento económico del león Asiático. Por eso su búsqueda se ha convertido en un tema principal dentro de la agenda de política exterior. Ecuador, el tercer país de Suramérica con más r...

  3. Identidad homosexual y contexto familiar heteroparental: implicaciones educativas para la subversión social

    Directory of Open Access Journals (Sweden)

    Marta Ceballos-Fernández

    2014-07-01

    Full Text Available Este estudio exploratorio ha sido emprendido con la finalidad de investigar, partiendo de los testimonios de personas homosexuales, el proceso de desarrollo de la identidad homosexual en el marco familiar heteroparental. Desde un criterio socioeducativo, se examina la respuesta del microsistema familiar ante este hecho, además de estudiar cómo se vive la homosexualidad en la familia de origen y otras cuestiones afines. En la investigación participan cuatro familias homoparentales españolas. A partir del empleo de una metodología cualitativa, apoyada en entrevistas en profundidad, efectuamos el pertinente análisis discursivo. Los resultados apuntan que la familia actúa como un factor de riesgo en la construcción de la identidad homosexual de los jóvenes, detectándose necesidades educativas que son discutidas al término del artículo.

  4. La violencia sexual asociada al conflicto y las implicaciones políticas de investigaciones recientes

    Directory of Open Access Journals (Sweden)

    Elisabeth Jean Wood

    2016-07-01

    Full Text Available La academia ha venido documentando un creciente número de modalidades de violencia sexual asociadas a los conflictos, sus causas y la gran variabilidad que existe en cuanto a su ejecución por parte de las organizaciones armadas. En este trabajo resumo los resultados de investigaciones recientes sobre dicha variabilidad, con énfasis en los hallazgos que contradicen o complican las creencias populares. Luego, presento varias interpretaciones sobre la afirmación de que esa violencia es parte de una continuidad entre tiempos de paz y de guerra. Después de analizar las investigaciones recientes sobre las dinámicas internas de las organizaciones armadas, sugiero que las violaciones sexuales a gran escala a menudo ocurren como una práctica, en vez de una estrategia. Por último, propongo algunos principios para guiar las políticas en vista de las investigaciones recientes

  5. Libre comercio en norteamerica. Algunas explicaciones e implicaciones para México

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    Carlos GONZÁLEZ MARTÍNEZ

    2009-11-01

    Full Text Available RESUMEN: El autor exmina las oportunidades y retos que puede encontrar México en el tratado de Libre Comercio, firmado entre México, Estados Unidos y Canadá. Considera esta integración económica como parte de un proceso más amplio de reforma del Estado y replanteamiento de los vínculos económicos con el exterior.ABSTRACT: The author analizes the opportunities and challenges that the Free Trade Agreement signed by México, The United States and Canada represents for Mexico. This economic integration is considered as a part of a greater process of reform of the State, and of reconsideration of the external economic links of Mexico with abroad.

  6. SEGURIDAD ENERGÉTICA Y BIOCOMBUSTIBLE. Un acercamiento a sus implicaciones

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    Dilia Paola Gómez Patiño

    2008-01-01

    Full Text Available El presente artículo es una aproximación al debate sobre los biocombustibles, que involucra diferentes aristas de observación: el factor político, económico y ambiental, para entrelazarlos alrededor de la seguridad energética. Así, parte de la definición de seguridad energética, para esbozar los potenciales de este tipo con que cuenta la región suramericana, llegando entonces alo que significa hoy la geopolítica del petróleo y del gas, para concluir sobre la necesidad de un nuevo abordaje del tema y una nueva forma de ser asumida por los estados.

  7. Implicaciones epistemológicas de la gramática transformacional de Noam Chomsky

    OpenAIRE

    Torrado Pacheco, Rafael Eduardo; Pontificia Universidad Javeriana

    2016-01-01

    Se reconoce con frecuencia que la Gramática Generativa Transformacional de Noam es una revolución científica y epistemológica, no sólo de la ciencia lingüística, sino también en otras disciplinas afines. El presente trabajo quiere señalar algunos aspectos de dicho proceso y mostrar algunos elementos que permitan aproximarse a esa revolución científica. Después de una introducción que ubica el problema, se muestra en qué consiste la revolución científica de Chomsky, siguiendo las tesis de las ...

  8. Implicaciones educativas de la teoría sociocultural de Vigotsky

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    Ana Lupita Chaves Salas

    2001-01-01

    Full Text Available El propósito de este artículo es analizar la teo-ría sociocultural del Lev Vigotsky y reflexionar sobre lasimplicaciones educativas de dicha teoría en los procesosde enseñanza y aprendizaje que se organizan en la es-cuela. Con base en los postulados de Vigotsky, se invita alas docentes y los docentes a repensar su práctica peda-gógica y a promover cambios en su labor profesional conel fin de ofrecer una educación más contextualizada ysignificativa que ayude a formar personas críticas y crea-tivas que contribuyan a construir una sociedad más de-mocrática y solidaria

  9. El nuevo periodo de la Normalizacion Internacional Contable y sus implicaciones en Colombia

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    Ernesto Sierra González

    2002-06-01

    Full Text Available La preponderancia de la vertiente financiera del capital en la actual dinámica de la globalización se viene caracterizando por una profunda desregulación de los mercados de capitales con el objeto de garantizar su libre movilidad. Para conseguir que los recursos financieros circulen se requiere de información transparente y homogénea. En tal sentido, la actual discusión internacional sobre las Normas Internacionales de Contabilidad y la tendencia mundial de su incorporación, son necesarias en el contexto colombiano, a fin de discernir la conveniencia -o no- de dicha decisión, y el marco de cosas en el que, eventualmente, debería acogerse tal normativa; tales opciones son abordadas en el presente artículo.

  10. Usos de Internet en contextos educativos informales: implicaciones para la educación formal

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    Sebastiano Costa

    2014-07-01

    Full Text Available El uso de Internet ofrece un importante espacio para el desarrollo social, emocional y cognitivo de los jóvenes y ocupa gran parte de su tiempo libre. Por lo tanto, es muy importante observar algunas variables que contribuyen a mejorar su uso como fuente de información y conocimiento en contextos formales e informales. ¿Cómo, entonces, aprovechar el enorme potencial de esta herramienta para ayudar a las personas en su aprendizaje?, ¿cuáles son las características cognitivas y sociales que ayudan a utilizarla sin que les afecte negativamente?, ¿qué habilidades se necesitan para seleccionar y gestionar la información y la comunicación?, ¿qué tipos de usos de Internet suscitan aprendizaje y nuevas y diferentes relaciones? En una muestra de 191 sujetos se examinan las diferentes características entre los sujetos con alto y bajo nivel de uso. Los resultados muestran que los individuos con alto nivel de uso de Internet tienen una puntuación más alta en lo que se refiere a las características de extroversión y apertura. La investigación se basa en un marco teórico que parte del análisis del uso de en un contexto informal para llegar a una reflexión sobre las posibilidades y ventajas que pueden derivarse de su uso en la educación, y del conjunto de habilidades que es necesario desarrollar para utilizar y evaluar la información de manera crítica y analítica y para construir una mente abierta y una actitud independiente.

  11. Characteristics of adenovirus urethritis among heterosexual men and men who have sex with men: a review of clinical cases.

    Science.gov (United States)

    Samaraweera, Geethani R; Garcia, Katherine; Druce, Julian; Williams, Henrietta; Bradshaw, Catriona S; Fairley, Christopher K; Chow, Eric Pf; Denham, Ian M; Read, Timothy R H; Chen, Marcus Y

    2016-05-01

    The aim of this study was to characterise the clinical features of adenovirus urethritis in men and to compare the frequency of these between heterosexual men and men who have sex with men (MSM). This was a review of the clinical and laboratory information from men diagnosed with PCR-confirmed adenovirus urethritis at the Melbourne Sexual Health Centre between January 2006 and April 2014. 102 adenovirus urethritis cases were reported, among which 61 were heterosexual men and 41 MSM. Eighty-nine per cent (n=91) had signs of meatitis or conjunctivitis: 51% had meatitis only; 32% meatitis together with conjunctivitis and 6% with conjunctivitis only. The distribution of symptoms and signs was similar among heterosexual men and MSM (p values >0.1). Adenovirus was the sole pathogen found in 93% of cases, excluding gonorrhoea, chlamydia, Mycoplasma genitalium and herpes simplex virus. Only 37% had ≥5 polymorphs per high-power field from a urethral smear. Where samples were still available for adenoviral sequencing (n=20), all were subgroup D. The clinical features of adenovirus urethritis in men can be distinctive and aid diagnosis, distinguishing it from other treatable causes of male urethritis. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  12. IMPLICACIONES ÉTICAS, LEGALES, RELIGIOSAS Y SOCIOECONÓMICAS DE LA FECUNDACIÓN IN VITRO EN LA SALUD REPRODUCTIVA DE COSTA RICA

    Directory of Open Access Journals (Sweden)

    Grettel Campos Vargas

    2012-04-01

    Full Text Available Las técnicas emergentes de reproducción humana asistida, entre las que se encuentran la fecundación in vitro (FIV, suscitanuna serie de interrogantes científicas, éticas y sociales. En relación con el tema, Costa Rica ha generado controversia a nivelmundial, pues no ha legalizado dicha técnica de fecundación, ya que parte de argumentos jurídicos que, según la OPS y laCIDH, van en contra de los derechos humanos y reproductivos. Por la situación anterior es que a continuación se describenlas implicaciones éticas, legales, religiosas y socioeconómicas de la fecundación in vitro en la salud reproductiva de CostaRica. Se realizó una revisión y análisis documental. Se buscó información en las siguientes bases de datos: PubMed,Medline, Excelencia.net, EBSCO, Cochrane. Se encontraron referentes bibliográficos que enfocan los aspectos religiosos,legales, éticos y socioeconómicos, los cuales se analizaron a la luz de los derechos reproductivos y la implicación de estatécnica en la salud reproductiva de Costa Rica. La posición de los y las Enfermeras y el mundo científico, así como lasdecisiones tomadas por usuarios, deben estar acompañadas de una información amplia y correcta acerca de los métodos, delas oportunidades de embarazo y de los efectos secundarios que la FIV pueda acarrear, de modo que contemple tantocuidados preconcepcionales, prenatales y perinatales, de allí que el y la enfermera obstetra tiene un papel importante en laatención integral de la mujer durante el período prenatal, el parto y el postparto, así como de la asistencia inmediata yeficiente del recién nacido. Se concluye que la fecundación in vitro constituye una técnica médica que se utiliza en muchaspartes del mundo para tratar la esterilidad. Puede beneficiar a los usuarios individuales y a la sociedad en general, no sóloporque trata la esterilidad, sino porque también ofrece la posibilidad de evitar los defectos genéticos y mejora

  13. Policonsumo simultáneo de drogas en estudiantes de facultades de ciencias de la salud/ciencias médicas en siete universidades de cinco países de América Latina y un país del Caribe: implicaciones de género, legales y sociales

    Directory of Open Access Journals (Sweden)

    Andrés Herrera Rodríguez

    2012-01-01

    Full Text Available El objetivo de este estudio fue comprender los patrones de policonsumo simultáneo de sustancias psicoactivas y sus implicaciones de género, legales y sociales, en estudiantes de primer y segundo año de las facultades de ciencias de la salud/ciencias médicas, en siete universidades de cinco países latinoamericanos, Colombia, Nicaragua, Chile, Brasil y El Salvador, y un país caribeño, Jamaica. El diseño fue un corte transversal. Las combinaciones de alcohol + tabaco y de alcohol + marihuana fueron las mas reportadas en todas las universidades, a excepción de alcohol + tabaco en Jamaica. Los factores asociados al policonsumo más referidos fueron "tener relaciones sexuales inesperada" en la universidad de Brasil, "tener relaciones sexuales sin protección" en las universidades de Chile, Colombia y Nicaragua, "tener problemas con su pareja sentimental" en la universidad de Jamaica, y "ausentarse de clases" en la universidad de El Salvador. Tres entornos se relacionaron, de manera positiva o negativa, con el policonsumo simultáneo de sustancias psicoactivas: estudiantil, familiar y de comportamiento sexual.

  14. pacientes con falla cardiaca

    Directory of Open Access Journals (Sweden)

    Diana Marcela Achury Saldaña

    2007-01-01

    Full Text Available Objetivo: determinar la adherencia al tratamiento de pacientes con falla cardiaca hospitalizados, al aplicar un plan educativo quefomenta el autocuidado.Método: estudio cuasiexperimental (entrevistas enfermera-paciente realizado entre diciembre de 2004 y mayo de 2006, con unamuestra de 50 pacientes seleccionados por conveniencia. Se diseñó un instrumento para evaluar los comportamientos de los pacientes,con base en algunos resultados de la adherencia y sus respectivos indicadores de la taxonomía NOC (Nursing out comes classification. Laadherencia al tratamiento fue medida en dos momentos: el primero durante la hospitalización, seguido de la aplicación del plan educativoantes del alta, que proporcionaba información en el manejo de su enfermedad desde una dimensión física, psicológica y social quepromueve el autocuidado; y el segundo un mes después del alta en su domicilio.Resultados: diferencias estadísticamente significativas (P=0,0001 que demuestran cómo mediante la capacitación al paciente enel manejo de su tratamiento farmacológico y no farmacológico, el establecimiento de una sana relación entre el profesional de enfermeríay el paciente, y la participación de la familia, se logra una total adherencia al tratamiento.Conclusiones: para lograr una adherencia total del paciente con falla cardiaca al tratamiento es necesario un proceso educativo y unseguimiento continuo y personalizado que motive permanentemente al paciente y se le reconozca el papel protagónico en su cuidado y manejo de la enfermedad.

  15. The organotypic multicellular spheroid is a relevant three-dimensional model to study adenovirus replication and penetration in human tumors in vitro

    NARCIS (Netherlands)

    Grill, Jacques; Lamfers, Martine L. M.; van Beusechem, Victor W.; Dirven, Clemens M.; Pherai, D. Shareen; Kater, Mathijs; van der Valk, Paul; Vogels, Ronald; Vandertop, W. Peter; Pinedo, Herbert M.; Curiel, David T.; Gerritsen, Winald R.

    2002-01-01

    The use of adenoviruses for gene transfer and as oncolytic agents is currently receiving widespread attention. As specific constraints to adenovirus distribution and spread cannot be studied in cell cultures, there is a need for an in vitro three-dimensional (3D) model mimicking the in vivo biology

  16. Las implicaciones de la convergencia económica en la industria del periodismo: el caso de Grupo Imagen

    Directory of Open Access Journals (Sweden)

    María Elena de Jesús Meneses Rocha

    2010-01-01

    Full Text Available El objetivo de este artículo es dar cuenta y analizar las transformaciones que ocurren a causa de la convergencia económica y corporativa en la industria periodística, así como sus implicaciones en la cultura profesional de los hasta ahora agentes validados socialmente para informar, es decir, los periodistas; también se analizan las consecuencias en la calidad de la información que reciben los ciudadanos de un país en proceso de consolidación democrática como es el caso de México proveniente los medios que han adoptado reingenierías convergentes, basadas en la digitalización de la base material de la producción noticiosa. Para ello, se toma como caso de estudio el Grupo Imagen, el primero en México en haber adoptado una reingeniería convergente, que a finales de la primera década del siglo parece ser una tendencia mundial en la industria del periodismo.

  17. Creando subjetividades laborales: implicaciones del discurso psicológico en el mundo del trabajo y las organizaciones

    Directory of Open Access Journals (Sweden)

    Verónica Andrade

    2013-01-01

    Full Text Available El presente documento tiene como propósito explorar aportes de la psicología crítica al entendimiento de las dinámicas que se mueven en el mundo del trabajo y las organizaciones, y algunas críticas que esta aporta a la psicología organizacional y del trabajo moderna. Para tal fin, se plantean algunos elementos conceptuales de base para clarificar la acepción al respecto del concepto subjetividad y las implicaciones de la psicología crítica; igualmente se exponen algunas reflexiones que explicitan las formas en que el discurso psicológico -reflejado además en prácticas- está embebido en el mundo del trabajo y las organizaciones, y que en gran medida contribuye a construir las subjetividades de personas que trabajan, y las relaciones que se establecen al interior de las organizaciones de trabajo.

  18. Trayectorias de persistencia y abandono de estudiantes universitarios no convencionales: implicaciones para la orientación

    Directory of Open Access Journals (Sweden)

    Pilar Figuera Gazo

    2015-01-01

    Full Text Available Uno de los objetivos de la política universitaria a nivel nacional e internacional ha sido la diversificación de las vías de entrada a la universidad para favorecer el acceso más generalizado a la educación superior. Sin embargo, el establecimiento de políticas de acceso debe ir acompañado de medidas para garantizar la permanencia en el sistema. La orientación es uno de los instrumentos más significativos. El artículo presenta los resultados de una investigación longitudinal sobre los procesos de transición y persistencia de una cohorte de estudiantes de ADE y Pedagogía de la Universidad de Barcelona que iniciaron los estudios en el año 2010. Los datos confirman que las situaciones de retraso académico, fracaso y abandono son significativamente superiores entre los estudiantes que accedieron a través de las vías no convencionales (específicamente aquellos procedentes de las vías CFGS, mayores de 25 años y 45 años. A partir de los resultados procedentes de las bases de datos institucionales y de los cuestionarios de seguimiento, se discute las implicaciones para el establecimiento de acciones de orientación y tutoría que contribuyan a la verdadera equidad del sistema.

  19. Reforma, cultura democrática y transversalidad Implicaciones en la relación universidad-sociedad

    Directory of Open Access Journals (Sweden)

    Suleima Bustamante Uzcátegui

    2007-05-01

    Full Text Available El propósito de este estudio es analizar los procesos de:reforma, diversidad, cultura democrática y transversalidad, y sus implicaciones en la relación universidad y sociedad. Estudio que se realiza bajo la modalidad del paradigma crítico reflexivo, en tanto que el nuevo ordenamiento social hace de las universidades, entidades más complejas, de variadas funciones, de mayor consumo de recursos, diluyendo su identidad en nuevas perspectivas. Se evidencia al currículo como el eje central del proceso de reforma, centrado en valores, el cual abre la posibilidad de abordar aspectos vitales tanto individual como grupal, transferibles a la sociedad en su conjunto. La reforma implica la diversidad y la transversalidad, reflejada en la concreción de un conjunto de medidas y de acciones, que reconoce la heterogeneidad de los grupos, de los valores, de la solidaridad, la tolerancia y el respeto a lo individual. La discusión revela el compromiso de las universidades para desarrollar un profundo trabajo epistemológico de investigación y redefinición de su actuar, como mecanismo para conectar los saberes a la complejidad del tejido de una realidad impregnada por los cambios, la complementariedad y la multiculturalidad.

  20. Relaciones interpersonales en niños y jóvenes con trastornos del espectro del autismo y discapacidad intelectual

    Directory of Open Access Journals (Sweden)

    Lucía Morán Suárez

    2015-06-01

    Full Text Available Las personas con discapacidad severa y profunda han sido un colectivo poco estudiado en el ámbito de la investigación y, menos aún, en las etapas de la infancia y adolescencia. Este estudio evalúa las relaciones interpersonales de una muestra de 55 personas con Trastorno del Espectro Autismo (TEA y discapacidad intelectual (DI entre 4 y 20 años. Para ello se utilizó la subescala de relaciones interpersonales de la Escala KidsLife (Gómez et al., en prensa que evalúa calidad de vida. Las puntuaciones más altas se obtuvieron en los ítems relacionados con la comunicación, mientras que las más bajas se observaron en los ítems relacionados con las interacciones sociales. Además, las personas con DI profunda obtuvieron puntuaciones significativamente más bajas que aquellos con mayores niveles de funcionamiento. Se discute acerca de las implicaciones del estudio y se realizan recomendaciones específicas para mejorar las prácticas profesionales y organizacionales dirigidas a la mejora de la calidad de vida.

  1. Deficiencia de zinc y sus implicaciones funcionales Zinc deficiency and its functional implications

    Directory of Open Access Journals (Sweden)

    JORGE L ROSADO

    1998-03-01

    Full Text Available El presente trabajo tiene por objeto revisar los aspectos teóricos y los estudios realizados en México que sugieren la existencia de la deficiencia moderada de zinc en niños de población rural, así como algunas de las consecuencias de dicha deficiencia en la salud. El zinc es un nutrimento indispensable para el organismo de los humanos y juega un papel importante en una serie de procesos metabólicos: participa en el sitio catalítico de varios sistemas enzimáticos; participa como ion estructural en membranas biológicas, y guarda una estrecha relación con la síntesis de proteínas, entre otras cosas. Es por esto que la deficiencia de zinc está asociada con consecuencias importantes en la salud y la funcionalidad de los individuos, especialmente durante las primeras etapas de la vida. De relevancia para México es la existencia de una deficiencia moderada de zinc en los niños y las consecuencias que ésta pueda tener en la salud de los mismos. Los estudios realizados sugieren que la deficiencia moderada de zinc se presenta asociada con la ingestión de dietas basadas en alimentos de origen vegetal, las cuales contienen cantidades importantes de inhibidores de la absorción de zinc. Este tipo de dietas se consume habitualmente en las zonas rurales y en la población marginal de las ciudades en el país. Entre las consecuencias más importantes de esta deficiencia se encontró un aumento en la presencia de enfermedades infecciosas, especialmente de diarrea, y posibles alteraciones en el desarrollo de la capacidad cognoscitiva.The purpose of this article is to review theoretical aspects and research performed in Mexico suggesting the existence of marginal zinc deficiency in rural children and its consequences on health. Zinc is an indispensable nutrient for humans since it plays an important role in several metabolic pathways: it participates in the catalytic site of several enzymes, as a structural ion of biological membranes and is

  2. Polimorfismos del receptor adrenérgico b1 y sus implicaciones farmacodinámicas

    Directory of Open Access Journals (Sweden)

    Ignacio Rodríguez

    2000-02-01

    Full Text Available

    Introducción: Los betabloqueadores son uno de los grupos farmacológicos que cuentan con mayor efectividad clínica comprobada; se ha demostrado que disminuyen la mortalidad y morbilidad por enfermedad cerebrovascular, eventos coronarios y falla cardíaca. Todos estos medicamentos tienen como común denominador el antagonismo de los receptores adrenérgicos b1, los cuales, se encuentran ubicados principalmente en cardiomiocitos, células yuxtaglomerulares y adipocitos; donde producen, en respuesta al estimulo por catecolaminas, aumento de la contractilidad y frecuencia cardíacas, liberación de renina y lipólisis respectivamente. Recientemente se descubrieron dos polimorfismos en este receptor. El primero de ellos, una sustitución de A por G en el nucleótido 145, genera un cambio del aminoácido Serina por Glicina en la posición 49 (Gly49Ser. El segundo polimorfismo, una sustitución de G por C en el nucleótido 1165, produce un cambio del aminoácido Arginina por Glicina en la posición 389 (Arg389Gly ubicado en la cola citoplasmática del receptor, que es una de las regiones de interacción con la proteína G(1. Estudios in vitro con las dos variantes polimórficas Arg389Gly del receptor, han demostrado que los receptores con Arg389 Tejido adiposo como glándula endocrina. Implicaciones fisiopatológicas.

    Directory of Open Access Journals (Sweden)

    Illovis Ramírez Arteaga

    2011-08-01

    Full Text Available

    El tejido adiposo se considera en la actualidad como un órgano con importante función endocrina; distinguiéndose dos tipos: el pardo y el blanco. El tejido adiposo es capaz de secretar diversas sustancias conocidas como adipocinas que se encuentran implicadas en la regulación del peso corporal, en el sistema inmune, en la función vascular y en la sensibilidad insulínica. En la obesidad, principalmente visceral, se encuentran aumentadas las adipocinas proinflamatorias. Estas se asocian a la inflamación clínica y subclínica, a la insulinorresistencia, al estrés oxidativo y a la lesión endotelial; desarrollan un papel clave en el síndrome metabólico e incrementan el riesgo cardiometabólico. El objetivo de la presente revisión bibliográfica ha sido exponer las características morfofuncionales del tejido adiposo y los mecanismos que lo vinculan con la génesis de diversas enfermedades.

    ADIPOSE TISSUE AS AN ENDOCRINE GLAND. PATHOPHYSIOLOGICAL IMPLICATIONS.

    Adipose tissue is currently recognized as an organ with an important endocrine function. Two main types of adipose tissue are distinguished: the brown and the white. Adipose tissue can secrete a variety of substances known as adipokines that are implicated in the regulation of body weight, the immune system and the vascular functioning and the insulin sensitivity. In cases of obesity (mainly visceral obesity, proinflammatory adipokines are increased. They are commonly associated with clinical and subclinical inflammation, insulin resistance, oxidative stress and endothelial injury. Proinflammatory adipokines also play a key role in the metabolic syndrome and increase cardiometabolic risk. The present review is aimed at exposing the morphofunctional characteristics of adipose tissue and the mechanisms that link this tissue to the origin of a variety

  3. Implicaciones Financieras de la Legislación Concursal: una Comparativa Europea

    Directory of Open Access Journals (Sweden)

    Myriam García Olalla

    2006-12-01

    Full Text Available El análisis de la reacción de los títulos de las empresas ante declaraciones legales de insolvencia ha sido desarrollado ampliamente en la literatura financiera. En este trabajo se adopta un enfoque diferente al tradicional estudio de eventos, tratando de contrastar si la reacción de los mercados viene condicionada por la orientación de la legislación concursal que regula las situaciones de insolvencia empresarial. Para ello se realiza un análisis con una muestra que incluye empresas con problemas de insolvencia en Alemania, España, Francia y el Reino Unido entre 1990 y 2002. Los resultados permiten concluir que la valoración de los títulos de las empresas se ve condicionada por el tipo de legislación concursal. Además, se contrasta cómo las empresas bajo sistemas más orientados hacia la protección de los acreedores (Reino Unido y Alemania antes de la reforma de 1994 presentan rentabilidades negativas mayores, debido a la transferencia de riqueza que se produce desde los accionistas hacia los aportantes de fondos ajenos a la empresa.The reaction of equity returns before bankruptcy filings has been developed widely in financial literature. Traditionally, event study methodology has been used in this kind of studies despite its limitations. Therefore, we propose a different approach contrasting if the reaction of equity returns is correlated with the different bankruptcy codes among countries. We develop an analysis with a sample that includes companies with insolvency problems in Germany, Spain, France and the United Kingdom from 1990 to 2002. The results allow us to conclude that the type of bankruptcy law affects the valuation of equity. In addition, we show how the companies under creditor-oriented procedures (United Kingdom and Germany before the 1994 reform have greater negative returns, due to the wealth transference that takes place from the shareholders towards the bondholders.

  4. Nucleic acid sequences encoding D1 and D1/D2 domains of human coxsackievirus and adenovirus receptor (CAR)

    Science.gov (United States)

    Freimuth, Paul I.

    2010-04-06

    The invention provides recombinant human CAR (coxsackievirus and adenovirus receptor) polypeptides which bind adenovirus. Specifically, polypeptides corresponding to adenovirus binding domain D1 and the entire extracellular domain of human CAR protein comprising D1 and D2 are provided. In another aspect, the invention provides nucleic acid sequences encoding these domains and expression vectors for producing the domains and bacterial cells containing such vectors. The invention also includes an isolated fusion protein comprised of the D1 polypeptide fused to a polypeptide which facilitates folding of D1 when expressed in bacteria. The functional D1 domain finds application in a therapeutic method for treating a patient infected with a CAR D1-binding virus, and also in a method for identifying an antiviral compound which interferes with viral attachment. The invention also provides a method for specifically targeting a cell for infection by a virus which binds to D1.

  5. Cementos con cenizas volantes

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    Ossa M., Mauricio

    1984-03-01

    additions of 20 and 30% .

    Casi la generalidad de los estudios realizados sobre cementos con adición de cenizas volantes se refieren a sus características y comportamiento en pastas, morteros y hormigones, siempre en relación con aquéllos del cemento portland. Esta vez, se desarrolló un trabajo experimental orientado a relacionar entre sí los cementos con adiciones de cenizas volantes y de puzolana natural. Para ello se fabricaron a escala de laboratorio cementos de ambos tipos, empleando como materias primas comunes clinker y yeso y, como variables, diferentes porcentajes de las dos adiciones, que cumplieron previamente los requisitos normalizados en cuanto a sus actividades puzolánicas. La calidad de los cementos fabricados resultó adecuada y concordante con la del cemento portland-puzolánico obtenido a escala industrial con los mismos clinker, yeso y puzolana natural de este estudio. Posteriormente, se determinaron las características de los cementos experimentales y se confeccionaron morteros normales para la realización de ensayos físicos y mecánicos. Los resultados de ensayos indicaron que los cementos con adición de cenizas volantes (CCV requieren menos agua para consistencia normal, presentan tiempos de fraguado mayores y expansiones en autoclave menores que los cementos con adición de puzolana (CP. Los calores de hidratación a 7 y 28 días de edad fueron aproximadamente similares para ambos tipos de cemento. En morteros normales, los cementos CCV mostraron menor retracción de secado, mayor retentividad y mayor fluidez (para igual cantidad de agua que los cementos CP. En los ensayos de exudación se observó que ésta depende más de la finura que el tipo de adición. Finalmente, los ensayos mecánicos señalaron que las resistencias a compresión y flexotracción de los morteros con cementos CCV son menores a edades inferiores que 14 días (del orden de 5 a 10% a un día de edad, pero que a partir de entonces pasan a ser mayores que las de

  6. La estructura de las organizaciones educativas y sus múltiples implicaciones

    Directory of Open Access Journals (Sweden)

    Isabel Cantón Mayo

    2003-01-01

    Full Text Available El estudio de los centros educativos en el marco general de la teoría de las organizaciones ha permitido grandes avances en su conocimiento científico, en formas de abordar su estudio y en las investigaciones que se generan sobre las escuelas entendidas desde la perspectiva genérica organizacional y desde la perspectiva específica por ser organizaciones que educan. En este artículo se presentan y estudian nuevas formas estructurales que permitan aplicar, conocer y utilizar sus ventajas e innovaciones en los centros educativos, al servicio de una mejor calidad en la educación que se imparte en ellos, en la idea de proporcionar una mejor alineación con el entorno en el que están inmersos y en la dinámica cambiante de la sociedad del conocimiento. Desde las formas estructurales más tradicionales como la organización formal e informal, a las más innovadoras como la organización en red, en trébol, adhocrática, etc. pueden dar una visión de los nuevos modelos emergentes en las organizaciones educativas.

  7. Algunas implicaciones y problemas bioéticos del diagnóstico prenatal

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    Fernando Fabó

    2008-08-01

    Full Text Available Este artículo define y presenta en la primera parte una panorámica general sobre las técnicas de diagnosis prenatal, justificando la licitud del recurso a las mismas en base a los avances de la medicina fetal. Viene fuertemente cuestionada la validez de la PDG y se aclaran algunos puntos relativos a la indicación médica. En la segunda parte se profundizan los aspectos bioéticos, explicando los principios éticos para una diagnosis prenatal plenamente respetuosa del pequeño paciente (valor ético positivo de la diagnosis; proporcionalidad terapéutica; consenso informado; responsabilidad-libertad. Se insiste en la objeción de conciencia y se presentan algunas sentencias que permiten evidenciar algunos problemas jurídico-legales relacionados con la diagnosis prenatal. Las conclusiones orientan a un nuevo enfoque de la prevención de las enfermedades genéticas y malformativas.

  8. El debate del aborto y sus implicaciones en las legislaciones mundiales

    Directory of Open Access Journals (Sweden)

    Neus Sánchez Expósito

    2010-12-01

    Full Text Available El aborto ha sido siempre un tema complejo que, en la actualidad, sigue suscitando intensos debates desde planteamientos muy dispares y posturas irreconciliables. Detrás de las diferentes líneas argumentativas para legitimar o no la interrupción voluntaria de la gestación existe un análisis de salud sexual y reproductiva desde una óptica moral con una concepción determinada de la “maternidad” que condiciona la jurisprudencia de los diferentes países. Muchas legislaciones permiten el aborto por cuestiones de salud, pero la defensa de la libertad de las mujeres lo reivindica como un derecho humano. Abortion has been always a complex subject still carries on causing heated discussions from very disparate criteria and irreconcilable positions. Behind the different argumentative lines to legitimate, or not, the voluntary termination of pregnancy, there is an analysis on sexual and reproductive health from a moral viewpoint with a certain conception of “motherhood” that conditions all countries’ jurisprudence. Many legislations authorize abortion on heath matters, but the defense of the women’s freedom demands it as a human right.

  9. CAVEOLINA-1 implicaciones fisiológicas y patológicas

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    Vanesa Pereira-Prado

    Full Text Available Las caveolas son invaginaciones de la membrana plasmática conformada por proteínas denominadas caveolinas. De las tres isoformas de caveolina la más estudiada en el transcurso de los años es caveolina-1 (cav-1, quien juega un papel importante en la señalización celular y su gen CAV-1 corresponde a la familia de genes supresores tumorales. Debido a su rol dependiente del contexto en el cual se encuentra, la función y participación de cav-1 a nivel tumoral es compleja y aún permanece controvertida. Cav-1 interactúa con una serie de moléculas-receptores que regulan inicialmente los pasos de la transformación celular a la malignidad, participando a su vez en el ciclo celular, la angiogénesis, la remodelación de la matriz extracelular, la proliferación celular, entre otras. El estudio de esta molécula adquiere importancia en función a su utilidad como biomarcador asociado a diagnóstico, pronóstico y posible blanco terapéutico en procesos patológicos.

  10. IMPLICACIONES TERRITORIALES DE UNA GRAN OBRA HIDRÁULICA: EL EMBALSE DEL TRANCO (CABECERA DEL GUADALQUIVIR

    Directory of Open Access Journals (Sweden)

    Eduardo Araque Jiménez

    2012-01-01

    Full Text Available Situado en la cabecera del Guadalquivir, el embalse del Tranco fue durante muchos años el de mayor capacidad de almacenamiento de agua de toda Andalucía. Sus aportaciones resultaron fundamentales para el sostenimiento del regadío en el gran valle bético, al tiempo que con sus aguas pudo generarse una parte de la energía eléctrica que se precisaba para la modernización del aparato productivo andaluz y la mejora de las condiciones de vida en multitud de hogares en el valle del Guadalquivir. Frente a estas perspectivas tan halagüeñas, las aguas embalsadas en el Tranco apenas tuvieron repercusiones socioeconómicas positivas para la Sierra de Segura. Las expropiaciones masivas a las que dio origen tanto la construcción del embalse como la repoblación forestal que lo acompañó, propiciaron la emigración masiva de centenares de vecinos, muchas veces en condiciones de extrema precariedad. Por otro lado, las aguas del Tranco nunca se utilizaron para la expansión del regadío por algunos de los fértiles valles próximos al embalse, ni la electricidad generada a pie de presa llegó a los hogares serranos, que siguieron careciendo de este servicio durante muchos años.

  11. Contactos Sociales y Orientación Estratégica: algunas implicaciones para la RSC

    Directory of Open Access Journals (Sweden)

    Virginia Fernández Pérez

    2011-09-01

    Full Text Available Las redes sociales de los directivos suelen tener un claro reflejo en las decisiones estratégicas que guían a la firma a la hora de actuar. Éstas proporcionan alternativas y ayudan a conformar los marcos de referencia que se utilizan en la toma de decisiones. Todo ello influye en el tipo de orientación estratégica de la organización y por tanto, en el nivel de responsabilidad social corporativa (RSC que se desprende de las acciones directivas. Esto es debido a que los grupos de interés que forman parte de las mismas van a tener más probabilidades de ver cumplidas sus expectativas dependiendo del posicionamiento estratégico elegido finalmente por la empresa. Por todo esto, la propia estructura de la red que envuelve al directivo, que en este caso es estudiada desde dos de sus dimensiones, como son: la fortaleza (calidad y la diversidad (cantidad de los nexos que unen y conforman la red; puede condicionar definitivamente la orientación y el comportamiento estratégico y social de la organización. Cuanto mayor es el nivel de relaciones sociales, se suelen adoptar posicionamientos estratégicos más proactivos con mayor grado de desarrollo de ciertos principios de su RSC.

  12. Dual effects of adenovirus-mediated thrombopoietin gene transfer on hepatic oval cell proliferation and platelet counts

    International Nuclear Information System (INIS)

    Ichiba, Miho; Shimomura, Takashi; Murai, Rie; Hashiguchi, Koichi; Saeki, Toshiya; Yoshida, Yoko; Kanbe, Takamasa; Tanabe, Naotada; Tsuchiya, Hiroyuki; Miura, Norimasa; Tajima, Fumihito; Kurimasa, Akihiro; Hamada, Hirofumi; Shiota, Goshi

    2005-01-01

    Thrombopoietin (TPO) is the growth factor for megakaryocytes and platelets, however, it also acts as a potent regulator of stem cell proliferation. To examine the significance of TPO expression in proliferation of hepatic oval cells, the effect of adenovirus-mediated TPO gene transfer into livers of the Solt-Farber model, which mimics the condition where liver regeneration is impaired, was examined. Hepatic TPO mRNA peaked its expression at 2 days after gene transduction and then gradually decreased. The peripheral platelet number began to increase at 4 days (P < 0.05) and reached its plateau at 9 days (P < 0.01). Oval cells expressed c-Mpl, a receptor for TPO as well as immature hematopoietic and hepatocytic surface markers such as CD34 and AFP. The proliferating cell nuclear antigen-positive oval cells in rats into which adenovirus-TPO gene was transferred at 7 and 9 days were significantly greater than those in adenovirus-LacZ gene transferred (P < 0.05, each), and the total numbers of oval cells in the adenovirus-TPO gene transferred at 9 and 13 days were also significantly greater than those in adenovirus-LacZ gene transferred (P < 0.05, each). Expression of SCF protein was increased at 4, 7, and 9 days by TPO gene administration and that of c-Kit was increased at 4 and 7 days. These data suggest that adenovirus-mediated TPO gene transfer stimulated oval cell proliferation in liver as well as increasing peripheral platelet counts, emphasizing the significance of the TPO/c-Mpl system in proliferation of hepatic oval cells

  13. Epidemiology of adenovirus respiratory infections among hospitalized children in Seremban, Malaysia.

    Science.gov (United States)

    Foong Ng, Khuen; Kee Tan, Kah; Hong Ng, Boon; Nair, Pritiss; Ying Gan, Wan

    2015-07-01

    There is scarcity of data regarding epidemiology and clinical aspects of human adenovirus acute respiratory infection (ARI) among children in developing countries. Retrospective data on demographics, clinical presentation, outcomes and laboratory findings of 116 children admitted into Tuanku Jaafar Hospital in Seremban, Malaysia from 2012 to 2013 with documented diagnosis of community-acquired adenovirus ARI were collected and analyzed. Male to female ratio was 1.70. Median age was 14 (1-107) months. The commonest symptoms were fever (94.8%; 110/116), cough (82.8%, 96), rhinorrhea (63.8%; 74), interrupted feeding (66.4%; 77), diarrhea (33.6%; 39) and conjunctivitis (21.6%; 25). Mean temperature on admission was 38.4°C±0.9°C. Among all 116 subjects, 20.7% (24) needed oxygen supplementation, 57.8% (67) required intravenous hydration, 11.2% (13) were admitted into the pediatric intensive care unit and 6.9% (8) required mechanical ventilation. Only 1% (1/87) had positive blood culture (Streptococcus pneumoniae) among 87 who received antibiotic treatment. Case fatality rate was 2.6% (3/116) and 1.7% (2/116) developed bronchiolitis obliterans. Median length of hospital stay was 4 (1-50) days. Adenovirus ARI caused significant morbidity and substantial resource utilization among hospitalized Malaysian children. It should be considered in the differential diagnosis of infants below two years presenting with ARI associated with high fever. Antibiotics should not be prescribed as secondary bacterial infections are uncommon. © The Author 2015. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  14. ENDEMIC INFECTION OF STRANDED SOUTHERN SEA OTTERS (ENHYDRA LUTRIS NEREIS) WITH NOVEL PARVOVIRUS, POLYOMAVIRUS, AND ADENOVIRUS.

    Science.gov (United States)

    Siqueira, Juliana D; Ng, Terry F; Miller, Melissa; Li, Linlin; Deng, Xutao; Dodd, Erin; Batac, Francesca; Delwart, Eric

    2017-07-01

    Over the past century, the southern sea otter (SSO; Enhydra lutris nereis) population has been slowly recovering from near extinction due to overharvest. The SSO is a threatened subspecies under federal law and a fully protected species under California law, US. Through a multiagency collaborative program, stranded animals are rehabilitated and released, while deceased animals are necropsied and tissues are cryopreserved to facilitate scientific study. Here, we processed archival tissues to enrich particle-associated viral nucleic acids, which we randomly amplified and deeply sequenced to identify viral genomes through sequence similarities. Anelloviruses and endogenous retroviral sequences made up over 50% of observed viral sequences. Polyomavirus, parvovirus, and adenovirus sequences made up most of the remaining reads. We characterized and phylogenetically analyzed the full genome of sea otter polyomavirus 1 and the complete coding sequence of sea otter parvovirus 1 and found that the closest known viruses infect primates and domestic pigs ( Sus scrofa domesticus), respectively. We tested archived tissues from 69 stranded SSO necropsied over 14 yr (2000-13) by PCR. Polyomavirus, parvovirus, and adenovirus infections were detected in 51, 61, and 29% of examined animals, respectively, with no significant increase in frequency over time, suggesting endemic infection. We found that 80% of tested SSO were infected with at least one of the three DNA viruses, whose tissue distribution we determined in 261 tissue samples. Parvovirus DNA was most frequently detected in mesenteric lymph node, polyomavirus DNA in spleen, and adenovirus DNA in multiple tissues (spleen, retropharyngeal and mesenteric lymph node, lung, and liver). This study describes the virome in tissues of a threatened species and shows that stranded SSO are frequently infected with multiple viruses, warranting future research to investigate associations between these infections and observed lesions.

  15. Respiratory syncytial virus, adenoviruses, and mixed acute lower respiratory infections in children in a developing country.

    Science.gov (United States)

    Rodríguez-Martínez, Carlos E; Rodríguez, Diego Andrés; Nino, Gustavo

    2015-05-01

    There is growing evidence suggesting greater severity and worse outcomes in children with mixed as compared to single respiratory virus infections. However, studies that assess the risk factors that may predispose a child to a mixture of respiratory syncytial virus (RSV) and adenoviral infections, are scarce. In a retrospective cohort study, the study investigated the epidemiology of RSV and adenovirus infections and predictors of mixed RSV-adenoviral infections in young children hospitalized with acute lower respiratory infection in Bogota, Colombia, South America, over a 2-year period 2009-2011. Of a total of 5,539 children admitted with a diagnosis of acute lower respiratory infection, 2,267 (40.9%) who were positive for RSV and/or adenovirus were selected. Out the total number of cases, 1,416 (62.5%) infections occurred during the 3-month period from March to May, the first rainy season of Bogota, Colombia. After controlling for gender, month when the nasopharyngeal sample was taken, and other pre-existing conditions, it was found that an age greater than 6 months (OR:1.74; CI 95%:1.05-2.89; P = 0.030) and malnutrition as a comorbidity (OR:9.92; CI 95%:1.01-100.9; P = 0.049) were independent predictors of mixed RSV-adenoviral infections in the sample of patients. In conclusion, RSV and adenovirus are significant causes of acute lower respiratory infection in infants and young children in Bogota, Colombia, especially during the first rainy season. The identified predictors of mixed RSV-adenoviral infections should be taken into account when planning intervention, in order to reduce the burden of acute lower respiratory infection in young children living in the country. © 2015 Wiley Periodicals, Inc.

  16. Radioiodine uptake of undifferentiated thyroid cancer cells by adenovirus-mediated Na+/ I- symporter gene transfer

    Energy Technology Data Exchange (ETDEWEB)

    So, Y.; Lee, Y. J.; Shin, J. H.; Oh, H. J.; Chung, J. K.; Lee, M. C.; Cho, B. Y. [College of Medicine, Univ. of Seoul National, Seoul (Korea, Republic of); Lee, K. H. [Samsung Medical Center, Seoul (Korea, Republic of)

    2003-07-01

    To increase radioiodine uptake on undifferentiated thyroid cancer cell (ARO cells) by adenovirus-mediated human Na+/I- symporter (hNIS) gene transfer. Recombinant adenovirus Ad-hNIS was manufactured successfully. After transfecting Ad-hNIS on ARO cells, in vitro I-125 uptake and efflux studies were performed. For in vivo studies, 1.510'8 p.f.u. (50 1) of Ad-hNIS was injected into xenograft ARO tumors on the R thigh of BALB/c nu/nu mice (n=12), and same amount of normal saline was injected into xenograft ARO tumors on the L thigh. Two, 3, 4 and 6 days after intratumoral injection of Ad-hNIS, I-131 images (3 mice per day) were taken and xenograft tumors on both thighs were all excised. Total RNA was extracted from each tumor tissue and RT-PCR was performed to confirm the hNIS expression of Ad-hNIS injected xenograft ARO tumors. I-125 uptake of Ad-hNIS transfected ARO cells was increased up to 233 folds at 120 minutes in vitro. I-125 efflux study revealed rapid washout of I-125 from Ad-hNIS transfected ARO cells. On dynamic image, I-131 uptake of Ad-hNIS injected ARO tumor was continuously increased until 60 minutes. Mean count ratios of xenograft ARO tumors (R/L) of 60 minutes I-131 images at 2, 3, 4 and 6 days after Ad-hNIS injection were 2.85, 2.54, 2.31, and 2.18, each. On RT-PCR, hNIS expression of Ad-hNIS transfected ARO xenograft tumors was confirmed. Radioiodine uptake was successfully increased in ARO cells by adenovirus-mediated hNIs gene transfer both in vitro and in vivo.

  17. Induction of mesenchymal cell phenotypes in lung epithelial cells by adenovirus E1A.

    Science.gov (United States)

    Behzad, A R; Morimoto, K; Gosselink, J; Green, J; Hogg, J C; Hayashi, S

    2006-12-01

    Epithelial-mesenchymal transformation is now recognised as an important feature of tissue remodelling. The present report concerns the role of adenovirus infection in inducing this transformation in an animal model of chronic obstructive pulmonary disease. Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). These cells were characterised by PCR, immunocytochemistry, electron microscopy, and Western and Northern blot analyses. Electrophoretic mobility shift assays were performed in order to examine nuclear factor (NF)-kappaB and activator protein (AP)-1 binding activities. E1A-PLECs and E1A-NLFs positive for E1A DNA, mRNA and protein expressed cytokeratin and vimentin but not smooth muscle alpha-actin. Both exhibited cuboidal morphology and junctional complexes, but did not contain lamellar bodies or express surfactant protein A, B or C mRNAs. These two cell types differed, however, in their NF-kappaB and AP-1 binding after lipopolysaccharide stimulation, possibly due to differences in the expression of the subunits that comprise these transcriptional complexes. E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. It is postulated that this intermediate phenotype may play a major role in the remodelling of the airways in chronic obstructive pulmonary disease associated with persistence of adenovirus E1A DNA.

  18. Avian Adenoviruses Infections with Special Attention to Inclusion Body Hepatitis/ Hydropericardium Syndrome and Egg Drop Syndrome

    Directory of Open Access Journals (Sweden)

    Hafez Mohamed Hafez*

    2011-04-01

    Full Text Available The first avian adenovirus (AAV associated with clinical disease was isolated from an outbreak of respiratory disease in quail in 1950 (Olson, 1950. Since that time, AAVs have been found in all types and breeds of chickens and from a variety of other avian species. The infections may be asymptomatic or associated with several clinical and pathological conditions. Vertical transmission via the egg is the most common way of transmission. Also horizontal transmission through faeces, contaminated egg trays, crates and trucks play a role in the infection route. Studies have demonstrated the presence of antibodies in healthy poultry, and viruses have been isolated from normal birds. Avian adenoviruses in chickens are the etiological agents of 2 diseases known as inclusion body hepatitis (IBH and hydropericardium syndrome (HP. In some cases each condition is observed separately, however, recently the 2 conditions have frequently been observed as a single entity; therefore, the name hepatitis hydropericardium has been widely used to describe the pathologic condition. The syndrome is an acute disease of young chickens associated with anemia, haemorrhagic disorders, hydropericardium and high mortality. Egg-Drop-Syndrome (EDS is caused also by an adenovirus. The disease is characterised by a severe drop in egg production as well as the production of shell-less, thin-shelled, discoloured or misshapen eggs in apparently healthy birds. Ducks and geese are the natural host of the EDS virus. It was first described in chickens in the 1970s and spread to several countries world wide. The birds usually do not show any other signs of disease, and mortality is not expected. There is no specific treatment of the AAV infections. Active immunization by vaccination using an inactivated is wide spread.

  19. competencia con China

    Directory of Open Access Journals (Sweden)

    Elena de la Paz Hernández Águila

    2007-01-01

    Full Text Available El artículo ofrece un diagnóstico del desempeño de la industria mexicana del calzado desde la década de los ochenta hasta la actualidad. Analiza la problemática que ha enfrentado esta rama industrial a partir del proceso de apertura comercial y de la competencia en su mercado interno con productos provenientes de países asiáticos, particularmente China. Problematiza al respecto los retos y las perspectivas que a mediano plazo enfrentará este sector empresarial y sobre las posibilidades de competir en el mercado globalizado.

  1. Construir con Madera

    OpenAIRE

    Olabe-Velasco, F. (Fermín); Val-Hernández, Y. (Yolanda); Varela-de-la-Cruz, P. (Perla); Cabrero-Ballarín, J.M. (José Manuel)

    2010-01-01

    Guía divulgativa ‘Construir con madera’, elaborada por la Cátedra Madera de la Universidad de Navarra y el Gobierno de Navarra. La publicación pretende explicar de forma sencilla los beneficios y posibilidades de este material en la construcción, tanto en lo que respecta a su resistencia, comportamiento frente al fuego, durabilidad, capacidad de aislamiento, propiedades acústicas, estética, respeto al medio ambiente y sostenibilidad como fuente de energía. A modo de ejemplo, en la ...

  2. Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China

    Directory of Open Access Journals (Sweden)

    Zhu Yuan-Mao

    2011-12-01

    Full Text Available Abstract Background Bovine adenovirus type 3 (BAV-3 belongs to the Mastadenovirus genus of the family Adenoviridae and is involved in respiratory and enteric infections of calves. The isolation of BAV-3 has not been reported prior to this study in China. In 2009, there were many cases in cattle showing similar clinical signs to BAV-3 infection and a virus strain, showing cytopathic effect in Madin-Darby bovine kidney cells, was isolated from a bovine nasal swab collected from feedlot cattle in Heilongjiang Province, China. The isolate was confirmed as a bovine adenovirus type 3 by PCR and immunofluorescence assay, and named as HLJ0955. So far only the complete genome sequence of prototype of BAV-3 WBR-1 strain has been reported. In order to further characterize the Chinese isolate HLJ0955, the complete genome sequence of HLJ0955 was determined. Results The size of the genome of the Chinese isolate HLJ0955 is 34,132 nucleotides in length with a G+C content of 53.6%. The coding sequences for gene regions of HLJ0955 isolate were similar to the prototype of BAV-3 WBR-1 strain, with 80.0-98.6% nucleotide and 87.5-98.8% amino acid identities. The genome of HLJ0955 strain contains 16 regions and four deletions in inverted terminal repeats, E1B region and E4 region, respectively. The complete genome and DNA binding protein gene based phylogenetic analysis with other adenoviruses were performed and the results showed that HLJ0955 isolate belonged to BAV-3 and clustered within the Mastadenovirus genus of the family Adenoviridae. Conclusions This is the first study to report the isolation and molecular characterization of BAV-3 from cattle in China. The phylogenetic analysis performed in this study supported the use of the DNA binding protein gene of adenovirus as an appropriate subgenomic target for the classification of different genuses of the family Adenoviridae on the molecular basis. Meanwhile, a large-scale pathogen and serological epidemiological

  3. Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection

    DEFF Research Database (Denmark)

    Carson, Christian T; Orazio, Nicole I; Lee, Darwin V

    2009-01-01

    The protein kinases ataxia-telangiectasia mutated (ATM) and ATM-Rad3 related (ATR) are activated in response to DNA damage, genotoxic stress and virus infections. Here we show that during infection with wild-type adenovirus, ATR and its cofactors RPA32, ATRIP and TopBP1 accumulate at viral...... during virus infection, which is independent of Mre11 nuclease activity and recruitment of RPA/ATR/ATRIP/TopBP1. Unlike other damage scenarios, we found that ATM and ATR signaling are not dependent on each other during infection. We identify a region of the viral E4orf3 protein responsible...

  4. ZEB1 limits adenoviral infectability by transcriptionally repressing the Coxsackie virus and Adenovirus Receptor

    Directory of Open Access Journals (Sweden)

    Lacher Markus D

    2011-07-01

    Full Text Available Abstract Background We have previously reported that RAS-MEK (Cancer Res. 2003 May 1;63(9:2088-95 and TGF-β (Cancer Res. 2006 Feb 1;66(3:1648-57 signaling negatively regulate coxsackie virus and adenovirus receptor (CAR cell-surface expression and adenovirus uptake. In the case of TGF-β, down-regulation of CAR occurred in context of epithelial-to-mesenchymal transition (EMT, a process associated with transcriptional repression of E-cadherin by, for instance, the E2 box-binding factors Snail, Slug, SIP1 or ZEB1. While EMT is crucial in embryonic development, it has been proposed to contribute to the formation of invasive and metastatic carcinomas by reducing cell-cell contacts and increasing cell migration. Results Here, we show that ZEB1 represses CAR expression in both PANC-1 (pancreatic and MDA-MB-231 (breast human cancer cells. We demonstrate that ZEB1 physically associates with at least one of two closely spaced and conserved E2 boxes within the minimal CAR promoter here defined as genomic region -291 to -1 relative to the translational start ATG. In agreement with ZEB1's established role as a negative regulator of the epithelial phenotype, silencing its expression in MDA-MB-231 cells induced a partial Mesenchymal-to-Epithelial Transition (MET characterized by increased levels of E-cadherin and CAR, and decreased expression of fibronectin. Conversely, knockdown of ZEB1 in PANC-1 cells antagonized both the TGF-β-induced down-regulation of E-cadherin and CAR and the reduction of adenovirus uptake. Interestingly, even though ZEB1 clearly contributes to the TGF-β-induced mesenchymal phenotype of PANC-1 cells, TGF-β did not seem to affect ZEB1's protein levels or subcellular localization. These findings suggest that TGF-β may inhibit CAR expression by regulating factor(s that cooperate with ZEB1 to repress the CAR promoter, rather than by regulating ZEB1 expression levels. In addition to the negative E2 box-mediated regulation the minimal

  5. iTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections

    OpenAIRE

    Trinh, Hung V.; Grossmann, Jonas; Gehrig, Peter; Roschitzki, Bernd; Schlapbach, Ralph; Greber, Urs F.; Hemmi, Silvio

    2013-01-01

    Both isobaric tags for relative and absolute quantitation (iTRAQ) and label-free methods are widely used for quantitative proteomics. Here, we provide a detailed evaluation of these proteomics approaches based on large datasets from biological samples. iTRAQ-label-based and label-free quantitations were compared using protein lysate samples from noninfected human lung epithelial A549 cells and from cells infected for 24 h with human adenovirus type 3 or type 5. Either iTRAQ-label-based or lab...

  6. Molecular Identification of Adenoviruses Associated with Respiratory Infection in Egypt from 2003 to 2010

    Science.gov (United States)

    2014-01-30

    have been shown to occur frequently in immunocompromised * Correspondence: annemgaynor@gmail.com 1U.S. Naval Medical Research Unit No. 3, Cairo, Egypt...treated with 10 μl of each of the following antibiotics : penicillin (100,000U/ml)/streptomycin (100,000 μg/ml), gentamicin (50 mg/ml)) and...Burchette JL Jr, Hale LP: Fatal disseminated adenovirus infections in immunocompromised patients. Am J Clin Pathol 2003, 120(4):575–583. 5. Hess BDJ

  7. Adenovirus-based vaccine against Listeria monocytogenes

    DEFF Research Database (Denmark)

    Jensen, Søren; Steffensen, Maria Abildgaard; Jensen, Benjamin Anderschou Holbech

    2013-01-01

    The use of replication-deficient adenoviruses as vehicles for transfer of foreign genes offers many advantages in a vaccine setting, eliciting strong cellular immune responses involving both CD8(+) and CD4(+) T cells. Further improving the immunogenicity, tethering of the inserted target Ag to MHC...... linked to Ii compared with vaccination with the unlinked vaccine. Studies using knockout mice demonstrated that CD8(+) T cells were largely responsible for this protection, which is mediated through perforin-dependent lysis of infected cells and IFN-γ production. Taking the concept a step further...

  8. Protection of Non-Human Primates against Rabies with an Adenovirus Recombinant Vaccine

    Science.gov (United States)

    Xiang, Z.Q.; Greenberg, L.; Ertl, H. C.; Rupprecht, C.E.

    2014-01-01

    Rabies remains a major neglected global zoonosis. New vaccine strategies are needed for human rabies prophylaxis. A single intramuscular immunization with a moderate dose of an experimental chimpanzee adenovirus (Ad) vector serotype SAd-V24, also termed AdC68, expressing the rabies virus glycoprotein, resulted in sustained titers of rabies virus neutralizing antibodies and protection against a lethal rabies virus challenge infection in a non-human primate model. Taken together, these data demonstrate the safety, immunogenicity, and efficacy of the recombinant Ad-rabies vector for further consideration in human clinical trials. PMID:24503087

  9. Inhibitory effect of Survivin promoter-regulated oncolytic adenovirus carrying P53 gene against gallbladder cancer.

    Science.gov (United States)

    Liu, Chen; Sun, Bin; An, Ni; Tan, Weifeng; Cao, Lu; Luo, Xiangji; Yu, Yong; Feng, Feiling; Li, Bin; Wu, Mengchao; Su, Changqing; Jiang, Xiaoqing

    2011-12-01

    Gene therapy has become an important strategy for treatment of malignancies, but problems remains concerning the low gene transferring efficiency, poor transgene expression and limited targeting specific tumors, which have greatly hampered the clinical application of tumor gene therapy. Gallbladder cancer is characterized by rapid progress, poor prognosis, and aberrantly high expression of Survivin. In the present study, we used a human tumor-specific Survivin promoter-regulated oncolytic adenovirus vector carrying P53 gene, whose anti-cancer effect has been widely confirmed, to construct a wide spectrum, specific, safe, effective gene-viral therapy system, AdSurp-P53. Examining expression of enhanced green fluorecent protein (EGFP), E1A and the target gene P53 in the oncolytic adenovirus system validated that Survivin promoter-regulated oncolytic adenovirus had high proliferation activity and high P53 expression in Survivin-positive gallbladder cancer cells. Our in vitro cytotoxicity experiment demonstrated that AdSurp-P53 possessed a stronger cytotoxic effect against gallbladder cancer cells and hepatic cancer cells. The survival rate of EH-GB1 cells was lower than 40% after infection of AdSurp-P53 at multiplicity of infection (MOI) = 1 pfu/cell, while the rate was higher than 90% after infection of Ad-P53 at the same MOI, demonstrating that AdSurp-P53 has a potent cytotoxicity against EH-GB1 cells. The tumor growth was greatly inhibited in nude mice bearing EH-GB1 xenografts when the total dose of AdSurp-P53 was 1 × 10(9) pfu, and terminal dUTP nick end-labeling (TUNEL) revealed that the apoptotic rate of cancer cells was (33.4 ± 8.4)%. This oncolytic adenovirus system overcomes the long-standing shortcomings of gene therapy: poor transgene expression and targeting of only specific tumors, with its therapeutic effect better than the traditional Ad-P53 therapy regimen already on market; our system might be used for patients with advanced gallbladder cancer and

  10. A Recombinant Adenovirus Expressing Ovine Interferon Tau Prevents Influenza Virus-Induced Lethality in Mice.

    Science.gov (United States)

    Martín, V; Pascual, E; Avia, M; Rangel, G; de Molina, A; Alejo, A; Sevilla, N

    2016-01-06

    Ovine interferon tau (IFN-τ) is a unique type I interferon with low toxicity and a broad host range in vivo. We report the generation of a nonreplicative recombinant adenovirus expressing biologically active IFN-τ. Using the B6.A2G-Mx1 mouse model, we showed that single-dose intranasal administration of recombinant Ad5-IFN-τ can effectively prevent lethality and disease induced by highly virulent hv-PR8 influenza virus by activating the interferon response and preventing viral replication. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  11. Los conflictos internacionales con Irán y Corea del Norte (I: el proceso negociador

    Directory of Open Access Journals (Sweden)

    Antonio Marquina

    2007-01-01

    Full Text Available El artículo detalla el proceso de negociación llevado a cabo para solucionar el conflicto creado por las pretensiones nucleares de Irán y la República Popular de Corea, así como el contexto político y de seguridad regional. El artículo está dividido en dos partes. La primera parte que se publica en este número detalla, en lo posible, el proceso negociador, las propuestas presentadas y las posiciones contrapuestas. Para su recto entendimiento se explican los dilemas y las líneas rojas establecidas por la Administración estadounidense, que van más allá de lo que establece el Tratado de No Proliferación; así como las razones de esta postura: el programa nuclear clandestino de Irán, la “admisión” de la existencia de un programa de enriquecimiento nuclear clandestino en el caso de la República Popular de Corea. Se analizan así los presupuestos, las carencias negociadoras, la falta de garantías de seguridad y la profunda desconfianza existente en estos dos Estados con respecto a las políticas e intenciones de los Estados Unidos. Una segunda parte explicará con más detalle las implicaciones políticas y de seguridad regional.

  12. Modelos de transposición de las Directivas 2002/91/CE y 2010/31/UE “Energy Performance Building Directive” en los Estados miembros de la UE. Consecuencias e implicaciones

    Directory of Open Access Journals (Sweden)

    García-Hooghuis, A.

    2013-09-01

    Full Text Available The 2002/91/EC Directive (Energy Performance Building Directive – EPBD (1, published by the European Commission and recast in the 2010/31/EU (2, requires European countries to meet certain minimum energy requirements in buildings and their systems. Each Member state is obliged to incorporate the Directive as national law(s, establishing the necessary conditions and procedures at a national level (3. In the present document a compilation of energy efficiency standards for buildings across Europe has been made, analyzing the consequences and implications of the different transposition models of the EPBD adopted by each country. Furthermore, the methodologies most widely used for determining the energy efficiency of buildings, legal gaps and the possibilities for improvement in areas such as professional accreditations or financial incentives are also described.La Directiva 2002/91/CE (Energy Performance Building Directive – EPBD (1, publicada por la Comisión Europea y refundida en la Directiva 2010/31/UE (2, obliga a los países de Europa a cumplir con ciertos requisitos mínimos de eficiencia energética en los edificios y sus instalaciones. En ella se exige que cada Estado miembro incorpore la Directiva como legislación propia, estableciendo las condiciones y sistemáticas necesarias con carácter nacional (3. En el presente trabajo se efectúa una recopilación de las normas de eficiencia energética en la edificación a nivel Europeo, analizándose las consecuencias e implicaciones de los diferentes modelos de transposición de la EPBD adoptados por cada país. Además, se describen de modo general las metodologías más extendidas a la hora determinar la eficiencia energética de los edificios, los vacíos legales que presenta la normativa y las posibilidades de mejora en determinadas áreas, como acreditación de profesionales o incentivos financieros.

  13. Acercamiento a las implicaciones existentes entre alimentación, calidad de vida y hábitos de vida saludables en la actualidad (Approaching existing implications between food, quality of life and healthy lifestyles today

    Directory of Open Access Journals (Sweden)

    Víctor Muñoz-Sánchez

    2015-05-01

    Full Text Available Resumen: En el presente artículo se realiza una revisión bibliográfica sobre el concepto de calidad de vida. En este recorrido bibliográfico se muestran las distintas corrientes teóricas que han influido en el desarrollo del concepto de calidad de vida así como la estrecha vinculación del concepto con diferentes modelos económicos y productivos. También se revisan sus implicaciones con la salud y en lo referente los hábitos de alimentación y vida saludable. En segundo lugar, se describe y reflexiona sobre los principales resultados de los trabajos de investigación contenidos en el presente monográfico. Por último se ofrecen conclusiones sobre el impacto del cambio de modelo productivo y de valores así como el de la actual situación estructural de crisis sobre; las condiciones de producción de productos de Denominación de Origen Protegida, las transformaciones del sistema alimentario español, los hábitos saludables de vida y alimentación de las personas mayores, los problemas de distribución de productos alimentarios en zonas rurales y la influencia de la actual socialización en hábitos de consumo alimentario en la distorsión de la autopercepción de la imagen del consumidor.Abstract: In this article a literature review on the concept of quality of life takes place. In this bibliographic tour the different theoretical currents that have influenced the development of the concept of quality of life and the close relationship of the concept with different economic and productive models shown. Health implications are also reviewed and regarding the eating habits and healthy life. Second, it describes and reflects on the main results of the research contained in this monograph. Finally conclusions are offered on the impact of changing production model and values and the current structural crisis on the conditions of production of Protected Designation of Origin, the transformations of Spanish food system, healthy habits of

  14. Evolución del concepto de desarrollo e implicaciones en el ámbito territorial: experiencia desde Cuba

    Directory of Open Access Journals (Sweden)

    Francisco Ángel Becerra Lois

    2005-01-01

    su desarrollo integral. Se ofrecen pautas generales para argumentar con cientificidad y con base en las teorías del desarrollo cuáles son los componentes o factores que subyacen tras la dinámica de desarrollo socioeconómico a escala territorial "preferentemente en el ámbito de localidad", durante un tiempo razonable.

  15. La empresa familiar en la Ley de Sociedades de Capital: implicaciones fiscales

    Directory of Open Access Journals (Sweden)

    Alfonso García Moncó

    2011-06-01

    Full Text Available La Sociedad Limitada Nueva Empresa puede ser el vehículo idóneo para la adopción de la forma societaria por una empresa familiar. El Real Decreto Legislativo 1/2010, de 3 de julio que aprueba el Texto Refundido de la Ley de Sociedades de Capital incorpora en su articulado y en sus disposiciones adicionales importantes ventajas para las SLNE, tanto para facilitar su constitución, como para flexibilizar su funcionamiento y reducir los costes fiscales de su creación. Las empresas familiares de reducida dimensión pueden beneficiarse de este marco jurídico y fiscal que, en estos momentos, no tiene comparación con ninguna otra modalidad societaria. Sin embargo, esta realidad es, en gran parte, desconocida porque no se han explicado las posibilidades que contiene la Ley de Sociedades de Capital al respecto y sus consecuencias fiscales.The New Enterprise Limited Company can be an ideal vehicle for the adoption of the corporate form by a family business. The Royal Decree 1/2010 of 3 July, which approves the revised text of the Capital Company Act, includes in its provisions and its additional provisions for SLNE important advantages, both to facilitate their establishment, as to ease operation and reduce the fiscal costs of its creation. The small size family businesses can benefit from the legal and fiscal framework at the moment, is unmatched by any other corporate form. However, this reality is largely unknown because they have not explained the possibilities contained in the Companies Act and Capital about the tax consequences.

  16. Entrevista con Giovanni Levi

    Directory of Open Access Journals (Sweden)

    Monica Oliveira

    2017-06-01

    Full Text Available En esta entrevista, Giovanni Levi - como un conocedor del tema de Familia - realiza una importante evaluación sobre el actual estado de las investigaciones realizadas en el Brasil y em el exterior. Con estilo franco, agudo y lucido critica las visiones tradicionales y sus ilusiones ypropone nuevos conceptos y métodos. La historia de la familia debería ceder espacio para el estudio de las redes relacionales o de los mundos relacionales. De la misma forma, la historia cuantitativa debería abrir espacio para el estudio de las cualidades. Ya con relación a la historia de las elites, tan estudiada y reproducida en una diversidad de trabajos, que deberíase mirar en otra perspectiva. Es decir, no mirar a las reglas sociales predeterminadas, sino a los desvíos y a las variaciones. Levi defiende que los historiadores deben trascender a los documentos que se encuentran fácilmente y que pueden fortalecer perspectivas deformadas y esequilibradas de la sociedad. Para él, los historiadores deben esforzarse por estudiar a aquellos grupos que dejaron pocos rastros documentales. En ese esfuerzo existiría una nueva mirada sobre la historia de la familia.

  17. Entrevista con Patricia Ariza

    Directory of Open Access Journals (Sweden)

    Esperanza Londoño La Rotta

    2017-01-01

    Full Text Available Pensamiento, Palabra y Obra entrevista a una artista, feminista y activista política, quien como mujer y artista ha permitido pensar el arte más allá de un simple espectáculo. Toda una vida dedicada al teatro y a darle voz, a través de sus obras, a víctimas del conflicto colombiano, defensora de derechos humanos; además de hacer evidente en su vida y a través de la plataforma “Artistas por la paz”, las múltiples relaciones que se pueden establecer entre el arte, la construcción de paz y la resolución de conflictos. Hablamos en su casa, en medio del calor de la bienvenida con Patricia Ariza, directora del festival alternativo de teatro, de Mujeres en Escena y de la Corporación Colombiana de Teatro, entre otras muchas actividades que voluntariamente su espíritu libertario ha asumido. Esta entrevista se realizó antes del 2 de octubre, pero con la revisión de los acuerdos que propició el plebiscito ganado por una ínfima minoría por el no, sigue siendo vigente este planteamiento.

  18. Estudio anatómico de la rama superficial del nervio radial, implicaciones quirúrgicas

    Directory of Open Access Journals (Sweden)

    Enrique Vergara Amador

    2010-07-01

    Full Text Available Antecedentes. Conocer la anatomía de la rama superficial del nervio radial, es indispensable para la adecuada práctica de cirugías que involucran la mano y la muñeca. Objetivo. Determinar las relaciones del nervio radial y sus ramas con el tubérculo de Lister, la apófisis estiloides del radio y la distribución en el dorso de la mano. Material y métodos. Se disecaron 25 piezas de cadáveres frescos. Se identificó la rama superficial del nervio radial y se midió la distancia entre la salida del nervio y la apófisis estiloides del radio. Se identificaron las ramas del nervio en el antebrazo distal y en la muñeca y se midieron respecto a la apófisis estiloides y al tubérculo de Lister. Resultados. La rama superficial del nervio radial emergió en la región dorsal y radial del tercio distal del antebrazo entre el músculo braquiradialis y el extensor carpis radialis longus, a una distancia de 8,45 cm proximal a la apófisis estiloides. Su primera rama de división discurrió palmar a la apófisis estiloides radial a una distancia promedio de 0,74 cm respecto a la misma. El tronco principal se dividió en varias ramas terminales así: proximal a la apófisis estiloides del radio (28%, a nivel de la misma (12% y distal (60%. El patrón de distribución de las ramas nerviosas en la base de los dedos más frecuente fue el del primero, segundo y el lado radial del tercer dedo (56%. Discusión. Este estudio mostró la gran variabilidad de la rama superficial del nervio radial en el dorso de la mano y la alta probabilidad de daño del mismo durante los procedimientos abiertos, artroscópicos o procedimientos percutáneos en la muñeca.

  19. Implicaciones de las propuestas de rancière y arendt para leer las contraculturas

    Directory of Open Access Journals (Sweden)

    Diana-Milena Patiño-Niño

    2013-01-01

    Full Text Available La contracultura es un concepto acuñado por Roszak en 1968 que hace referencia al conjunto de aquellas expresiones culturales que difieren de lo que está establecido como «cultura oficial» y constituye el único medio para que un grupo de personas que viven bajo el paradigma de esa cultura oficial y que no se identifican con ella, le den un sentido al mundo en el que viven. De estas contraculturas se afirma que son el motor de los cambios culturales, en tanto resistencia, frente a los paradigmas hegemónicos culturales y cuyo éxito radica en la reconfiguración de dichos paradigmas. Sin embargo, hablar de cultura dominante y de ciertas contraculturas que se le oponen implica no solamente identificar y asignarle un lugar en la sociedad a la cultura hegemónica, sino también a esas contraculturas, borrando su carácter de contra. Para poder pensar las contraculturas en tanto tales, se podría extender al terreno de la culturalidad la invitación que nos hacen Arendt y Rancière respecto de la política. Esto nos llevaría a concebir la contracultura por fuera de las categorías y de las funciones que le han sido asignadas y nos permitiría hablar no de contraculturas sino de gestos contraculturales, los que se comprenderánno como expresiones que se inscriben dentro de un grupo en el que rigen unas ciertas normas (implícitas o explícitas respecto de comportamientos, modos de ser o modos de existir en comunidad, sino como apariciones inesperadas, incluso efímeras, que, en términos rancierianos (cuando se refiere a la política, irrumpen en un cierto trazado o reparto de lo sensible, entendiendo por esto una forma de contar que distribuye acciones y asigna jerárquicamente lugares y funciones, modos de ser, modos de hacer y de decir, modos de verse y modos de aparecer, correspondientes a cada lugar asignado

  20. Choquequirao en el siglo XVI: etnohistoria e implicaciones arqueológicas

    Directory of Open Access Journals (Sweden)

    2005-01-01

    Full Text Available Choquequirao au XVIe siècle : ethnohistoire et implications archéologiques Résumé: À travers l’étude de divers documents d’archives, ce travail reconstitue l’histoire coloniale du site archéologique de Choquequirao. Ce site, qui faisait tout d’abord partie d’une encomienda octroyée à Hernando Pizarro, devient partie intégrale de la réduction de Cachora, à la fin du XVIe siècle, avec les anciens villages de Sayhuite et Urco. Cet article démontre aussi que durant plus de 80 ans, les Espagnols ont concédé aux mêmes familles les charges de cacique de ces villages. Ces nouvelles données concernant l’histoire de Choquequirao amènent à se poser de nouvelles questions dans le domaine de l’archéologie. Este trabajo, a través del análisis de varias fuentes manuscritas, reconstituye la historia colonial del sitio arqueológico de Choquequirao. En primera instancia encomienda de Hernando Pizarro, este sitio fue reducido a fines del siglo XVI en el pueblo de Cachora, junto con los pueblos de Sayhuite y Urco. Este artículo demuestra también que los españoles concedieron el cargo de cacique de estos pueblos a las mismas familias durante más de 80 años. Estos nuevos conocimientos sobre la historia de Choquequirao llevan a plantear nuevas preguntas a la arqueología Choquequirao in the 16th century: ethnohistory and archaeological implications Abstract: Through the study of various archive documents, this work reconstructs the colonial history of the archaeological site of Choquequirao. This site, which first formed part of a Hernando Pizarro’s holding, was reduced together with the old villages of Sayhuite and Urcp to the locality of Cachora at the end of the 16th century. This article also shows that during more than 80 years, the Spaniards conceded the office of cacique of these villages to the same families. These new data concerning the history of Choquequirao bring to raise new questions for archaeology.

  1. Algunas implicaciones de la escritura ante lo imposible On implications of writing when facing the impossible

    Directory of Open Access Journals (Sweden)

    María Victoria Salinas

    2011-12-01

    Full Text Available En el presente artículo partimos del análisis de un escrito que retoma las preguntas de Job ante el sacrificio de millones de inocentes en el Holocausto. ¿Dónde estuvo Dios en Auschwitz?, se pregunta ZviKolitz, quien, a su modo, interpela a un Dios que en el momento de mayor dolor y desvalimiento oculta su rostro. Tal escrito, encontrado dentro de una botella en las ruinas del gueto de Varsovia, representa un testamento redactado en las postrimerías. Con lucidez, aunque apasionadamente, interpela a Dios, lo convoca y lo rescata transformándolo en Su Dios, rescatándose de este modo a sí mismo. Apoyándonos en estos escritos nos interrogamos sobre la función del Otro y la escritura cuando el goce atormenta al sujeto. Frente a los fenómenos de segregación, de reducción del otro a una pura nada, postulamos que lo que salva al hombre de esta reducción es la capacidad de transformase en un sujeto a través de rescatar a un Otro, no universal sino singular. Por último, nos preguntaremos qué relación existe entre el Otro, en este caso Dios, el sufrimiento del hombre y la escritura.This article begins analyzing a letter that brings back Job´s questions, this time regarding the slaughter of millions of innocent people in the Holocaust. Where was God in Auschwitz?, asks ZviKolitz, who, in his own way, questions a God who in times of pain and helplessness hides His face. Such writing, found inside a bottle in the ruins of the Warsaw ghetto, becomes a testament made at the end of life. Lucidly but passionately, he challenges God, calls for Him and turns Him into his own God, thus saving himself. This study is based on that posthumous letter to focus on Writing and the role of the Other when the enjoyment torments the subject. Against segregation phenomena, that turns victims to a pure nothingness, we postulate that what rescues a person from this reduction is the ability of becoming aSubject by turning the Other from universal to unique

  2. Contexto e implicaciones en la vivencia de mujeres autoras de un delito violento ejercido hacia su pareja. Un estudio desde la perspectiva de género

    OpenAIRE

    Alvarado Sánchez, Patricia

    2012-01-01

    En este proyecto se analiza el contexto e implicaciones de mujeres cualificadas como sujeto activo de un delito violento contra su pareja. La problematización del fenómeno investigado se enmarca en un contexto jurídico-social. Comprende los objetivos sobre a) Visualizar la dinámica relacional entre la mujer (género femenino) y su compañero varón (género masculino) previa al delito, b) Comprender los significados atribuidos al episodio y a las circunstancias del delito hacia su pareja, c) Ente...

  3. La mecánica cuántica de David Bohm: formalismo matemático e implicaciones epistemológicas

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    Rodrigo Siqueira-Batista

    2014-11-01

    Full Text Available La interpretación bohmiana de la Mecánica Cuántica representa una de las principales alternativas al modelo hegemónico propuesto por la Escuela de Copenhague, permitiendo una descripción determinista de los eventos ocurridos a nivel cuántico. El objetivo del presente artículo es presentar sucintamente sus principales aspectos matemáticos y conceptuales —y sus respectivas implicaciones epistemológicas.

  4. A recombinant E1-deleted porcine adenovirus-3 as an expression vector

    International Nuclear Information System (INIS)

    Zakhartchouk, Alexander; Zhou Yan; Tikoo, Suresh Kumar

    2003-01-01

    Replication-defective E1-deleted porcine adenoviruses (PAVs) are attractive vectors for vaccination. As a prerequisite for generating PAV-3 vectors containing complete deletion of E1, we transfected VIDO R1 cells (fetal porcine retina cells transformed with E1 region of human adenovirus 5) with a construct containing PAV-3 E1B large coding sequences under the control of HCMV promoter. A cell line named VR1BL could be isolated that expressed E1B large of PAV-3 and also complemented PAV214 (E1A+E1B small deleted). The VR1BL cells could be efficiently transfected with DNA and allowed the rescue and propagation of recombinant PAV507 containing a triple stop codon inserted in the E1B large coding sequence. In addition, recombinant PAV227 containing complete deletion of E1 (E1A+E1B small + E1B large ) could be successfully rescued using VR1BL cell line. Recombinant PAV227 replicated as efficiently as wild-type in VR1BL cells but not in VIDO R1 cells, suggesting that E1B large was essential for replication of PAV-3. Next, we constructed recombinant PAV219 by inserting green fluorescent (GFP) protein gene flanked by a promoter and a poly(A) in the E1 region of the PAV227 genome. We demonstrated that PAV219 was able to transduce and direct expression of GFP in some human cell lines

  5. Metabolic flux profiling of MDCK cells during growth and canine adenovirus vector production.

    Science.gov (United States)

    Carinhas, Nuno; Pais, Daniel A M; Koshkin, Alexey; Fernandes, Paulo; Coroadinha, Ana S; Carrondo, Manuel J T; Alves, Paula M; Teixeira, Ana P

    2016-03-23

    Canine adenovirus vector type 2 (CAV2) represents an alternative to human adenovirus vectors for certain gene therapy applications, particularly neurodegenerative diseases. However, more efficient production processes, assisted by a greater understanding of the effect of infection on producer cells, are required. Combining [1,2-(13)C]glucose and [U-(13)C]glutamine, we apply for the first time (13)C-Metabolic flux analysis ((13)C-MFA) to study E1-transformed Madin-Darby Canine Kidney (MDCK) cells metabolism during growth and CAV2 production. MDCK cells displayed a marked glycolytic and ammoniagenic metabolism, and (13)C data revealed a large fraction of glutamine-derived labelling in TCA cycle intermediates, emphasizing the role of glutamine anaplerosis. (13)C-MFA demonstrated the importance of pyruvate cycling in balancing glycolytic and TCA cycle activities, as well as occurrence of reductive alphaketoglutarate (AKG) carboxylation. By turn, CAV2 infection significantly upregulated fluxes through most central metabolism, including glycolysis, pentose-phosphate pathway, glutamine anaplerosis and, more prominently, reductive AKG carboxylation and cytosolic acetyl-coenzyme A formation, suggestive of increased lipogenesis. Based on these results, we suggest culture supplementation strategies to stimulate nucleic acid and lipid biosynthesis for improved canine adenoviral vector production.

  6. Generation of an adenovirus-parvovirus chimera with enhanced oncolytic potential.

    Science.gov (United States)

    El-Andaloussi, Nazim; Bonifati, Serena; Kaufmann, Johanna K; Mailly, Laurent; Daeffler, Laurent; Deryckère, François; Nettelbeck, Dirk M; Rommelaere, Jean; Marchini, Antonio

    2012-10-01

    In this study, our goal was to generate a chimeric adenovirus-parvovirus (Ad-PV) vector that combines the high-titer and efficient gene transfer of adenovirus with the anticancer potential of rodent parvovirus. To this end, the entire oncolytic PV genome was inserted into a replication-defective E1- and E3-deleted Ad5 vector genome. As we found that parvoviral NS expression inhibited Ad-PV chimera production, we engineered the parvoviral P4 early promoter, which governs NS expression, by inserting into its sequence tetracycline operator elements. As a result of these modifications, P4-driven expression was blocked in the packaging T-REx-293 cells, which constitutively express the tetracycline repressor, allowing high-yield chimera production. The chimera effectively delivered the PV genome into cancer cells, from which fully infectious replication-competent parvovirus particles were generated. Remarkably, the Ad-PV chimera exerted stronger cytotoxic activities against various cancer cell lines, compared with the PV and Ad parental viruses, while being still innocuous to a panel of tested healthy primary human cells. This Ad-PV chimera represents a novel versatile anticancer agent which can be subjected to further genetic manipulations in order to reinforce its enhanced oncolytic capacity through arming with transgenes or retargeting into tumor cells.

  7. Preclinical Safety Studies of Enadenotucirev, a Chimeric Group B Human-Specific Oncolytic Adenovirus

    Directory of Open Access Journals (Sweden)

    Sam Illingworth

    2017-06-01

    Full Text Available Enadenotucirev is an oncolytic group B adenovirus identified by a process of bio-selection for the ability to selectively propagate in and rapidly kill carcinoma cells. It is resistant to inactivation by human blood components, potentially enabling intravenous dosing in patients with metastatic cancer. However, there are no known permissive animal models described for group B adenoviruses that could facilitate a conventional approach to preclinical safety studies. In this manuscript, we describe our tailored preclinical strategy designed to evaluate the key biological properties of enadenotucirev. As enadenotucirev does not replicate in animal cells, a panel of primary human cells was used to evaluate enadenotucirev replication selectivity in vitro, demonstrating that virus genome levels were >100-fold lower in normal cells relative to tumor cells. Acute intravenous tolerability in mice was used to assess virus particle-mediated toxicology and effects on innate immunity. These studies showed that particle toxicity could be ameliorated by dose fractionation, using an initial dose of virus to condition the host such that cytokine responses to subsequent doses were significantly attenuated. This, in turn, supported the initiation of a phase I intravenous clinical trial with a starting dose of 1 × 1010 virus particles given on days 1, 3, and 5.

  8. Novel coronaviruses, astroviruses, adenoviruses and circoviruses in insectivorous bats from northern China.

    Science.gov (United States)

    Han, H-J; Wen, H-L; Zhao, L; Liu, J-W; Luo, L-M; Zhou, C-M; Qin, X-R; Zhu, Y-L; Liu, M-M; Qi, R; Li, W-Q; Yu, H; Yu, X-J

    2017-12-01

    Bats are considered as the reservoirs of several emerging infectious disease, and novel viruses are continually found in bats all around the world. Studies conducted in southern China found that bats carried a variety of viruses. However, few studies have been conducted on bats in northern China, which harbours a diversity of endemic insectivorous bats. It is important to understand the prevalence and diversity of viruses circulating in bats in northern China. In this study, a total of 145 insectivorous bats representing six species were collected from northern China and screened with degenerate primers for viruses belonging to six families, including coronaviruses, astroviruses, hantaviruses, paramyxoviruses, adenoviruses and circoviruses. Our study found that four of the viruses screened for were positive and the overall detection rates for astroviruses, coronaviruses, adenoviruses and circoviruses in bats were 21.4%, 15.9%, 20% and 37.2%, respectively. In addition, we found that bats in northern China harboured a diversity of novel viruses. Common Serotine (Eptesicus serotinu), Fringed long-footed Myotis (Myotis fimriatus) and Peking Myotis (Myotis pequinius) were investigated in China for the first time. Our study provided new information on the ecology and phylogeny of bat-borne viruses. © 2017 The Authors. Zoonoses and Public Health Published by Blackwell Verlag GmbH.

  9. Cryo-EM structures of two bovine adenovirus type 3 intermediates

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, Lingpeng; Huang, Xiaoxing; Li, Xiaomin [National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China); Xiong, Wei [State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Luo-jia-shan, Wuhan, Hubei 430072 (China); Sun, Wei; Yang, Chongwen; Zhang, Kai; Wang, Ying [National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China); Liu, Hongrong [College of Physics and Information Science, Hunan Normal University, Changsha, Hunan 410081 (China); Huang, Xiaojun; Ji, Gang; Sun, Fei [National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China); Zheng, Congyi, E-mail: cctcc202@whu.edu.cn [State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Luo-jia-shan, Wuhan, Hubei 430072 (China); Zhu, Ping, E-mail: zhup@ibp.ac.cn [National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China)

    2014-02-15

    Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are localized in the fivefold vertex region, while their facet structures are identical. The overall facet structure of BAd3 exhibits a similar structure to human Ads; however, BAd3 protein IX has a unique conformation. Mass spectrometry and cryo-electron tomography analyses indicate that one intermediate structure represents the stage during DNA encapsidation, whilst the other intermediate structure represents a later stage. These results also suggest that cleavage of precursor protein VI occurs during, rather than after, the DNA encapsidation process. Overall, our results provide insights into the mechanism of Ad assembly, and allow the first structural comparison between human and nonhuman Ads at backbone level. - Highlights: • First structure of bovine adenovirus type 3. • Some channels are located at the vertex of intermediate during DNA encapsidation. • Protein IX exhibits a unique conformation of trimeric coiled–coiled structure. • Cleavage of precursor protein VI occurs during the DNA encapsidation process.

  10. Adenovirus-mediated IL-12 gene therapy in combination with radiotherapy for murine liver cancer

    International Nuclear Information System (INIS)

    Wei Daoyan; Dai Bingbing; Wang Zhonghe; Chen Shishu

    2001-01-01

    Objective: To investigate the synergistic antitumor effects of adenovirus-mediated IL-12 gene therapy in combination with radiotherapy in mice bearing liver cancer. Methods: Balb/c mice bearing liver cancer received the treatment at day 1 with tumor local irradiation (TLI) of 20 Gy or mask irradiation when tumor size reached 0.6-1.0 cm. Within 1 hour after irradiation, adenovirus containing IL-12 gene or PBS was intra-tumor injected once a week. Forty-eight hours after the second injection, IFN-γ levels in sera and the supernatant of cultured spleen cells were assayed by ELISA, CTL activity of spleen cells was measured by 3 H-TdR release assay, and phenotypes of tumor-infiltrating lymphocytes were analysed by immunohistochemical staining. Results: The growth of tumors in animals treated with a combination of IL-12 gene therapy and TLI was inhibited more significantly than those with either single treatment (P + and CD8 + lymphocyte infiltration and tumor-specific cytolytic activities, and the levels of IFN-γ in sera were higher in IL-12 gene therapy and IL-12 gene therapy combined with TLI groups. Conclusion: These results suggest that IL-12 gene therapy combined with radiotherapy is more effective than both single treatment modalities and can induce specific antitumor immuno-response greatly

  11. An adenovirus vector incorporating carbohydrate binding domains utilizes glycans for gene transfer.

    Directory of Open Access Journals (Sweden)

    Julius W Kim

    Full Text Available Vectors based on human adenovirus serotype 5 (HAdV-5 continue to show promise as delivery vehicles for cancer gene therapy. Nevertheless, it has become clear that therapeutic benefit is directly linked to tumor-specific vector localization, highlighting the need for tumor-targeted gene delivery. Aberrant glycosylation of cell surface glycoproteins and glycolipids is a central feature of malignant transformation, and tumor-associated glycoforms are recognized as cancer biomarkers. On this basis, we hypothesized that cancer-specific cell-surface glycans could be the basis of a novel paradigm in HAdV-5-based vector targeting.As a first step toward this goal, we constructed a novel HAdV-5 vector encoding a unique chimeric fiber protein that contains the tandem carbohydrate binding domains of the fiber protein of the NADC-1 strain of porcine adenovirus type 4 (PAdV-4. This glycan-targeted vector displays augmented CAR-independent gene transfer in cells with low CAR expression. Further, we show that gene transfer is markedly decreased in cells with genetic glycosylation defects and by inhibitors of glycosylation in normal cells.These data provide the initial proof-of-concept for HAdV-5 vector-mediated gene delivery based on the presence of cell-surface carbohydrates. Further development of this new targeting paradigm could provide targeted gene delivery based on vector recognition of disease-specific glycan biomarkers.

  12. Adenovirus E1A/E1B Transformed Amniotic Fluid Cells Support Human Cytomegalovirus Replication

    Directory of Open Access Journals (Sweden)

    Natascha Krömmelbein

    2016-02-01

    Full Text Available The human cytomegalovirus (HCMV replicates to high titers in primary human fibroblast cell cultures. A variety of primary human cells and some tumor-derived cell lines do also support permissive HCMV replication, yet at low levels. Cell lines established by transfection of the transforming functions of adenoviruses have been notoriously resistant to HCMV replication and progeny production. Here, we provide first-time evidence that a permanent cell line immortalized by adenovirus type 5 E1A and E1B (CAP is supporting the full HCMV replication cycle and is releasing infectious progeny. The CAP cell line had previously been established from amniotic fluid cells which were likely derived from membranes of the developing fetus. These cells can be grown under serum-free conditions. HCMV efficiently penetrated CAP cells, expressed its immediate-early proteins and dispersed restrictive PML-bodies. Viral DNA replication was initiated and viral progeny became detectable by electron microscopy in CAP cells. Furthermore, infectious virus was released from CAP cells, yet to lower levels compared to fibroblasts. Subviral dense bodies were also secreted from CAP cells. The results show that E1A/E1B expression in transformed cells is not generally repressive to HCMV replication and that CAP cells may be a good substrate for dense body based vaccine production.

  13. Cryo-EM structures of two bovine adenovirus type 3 intermediates

    International Nuclear Information System (INIS)

    Cheng, Lingpeng; Huang, Xiaoxing; Li, Xiaomin; Xiong, Wei; Sun, Wei; Yang, Chongwen; Zhang, Kai; Wang, Ying; Liu, Hongrong; Huang, Xiaojun; Ji, Gang; Sun, Fei; Zheng, Congyi; Zhu, Ping

    2014-01-01

    Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are localized in the fivefold vertex region, while their facet structures are identical. The overall facet structure of BAd3 exhibits a similar structure to human Ads; however, BAd3 protein IX has a unique conformation. Mass spectrometry and cryo-electron tomography analyses indicate that one intermediate structure represents the stage during DNA encapsidation, whilst the other intermediate structure represents a later stage. These results also suggest that cleavage of precursor protein VI occurs during, rather than after, the DNA encapsidation process. Overall, our results provide insights into the mechanism of Ad assembly, and allow the first structural comparison between human and nonhuman Ads at backbone level. - Highlights: • First structure of bovine adenovirus type 3. • Some channels are located at the vertex of intermediate during DNA encapsidation. • Protein IX exhibits a unique conformation of trimeric coiled–coiled structure. • Cleavage of precursor protein VI occurs during the DNA encapsidation process

  14. A single exercise bout augments adenovirus-specific T-cell mobilization and function.

    Science.gov (United States)

    Kunz, Hawley E; Spielmann, Guillaume; Agha, Nadia H; O'Connor, Daniel P; Bollard, Catherine M; Simpson, Richard J

    2018-04-30

    Adoptive transfer of virus-specific T-cells (VSTs) effectively treats viral infections following allogeneic hematopoietic stem cell transplantation (alloHSCT), but logistical difficulties have limited widespread availability of VSTs as a post-transplant therapeutic. A single exercise bout mobilizes VSTs specific for latent herpesviruses (i.e. CMV and EBV) to peripheral blood and augments their ex vivo expansion. We investigated whether exercise exerts similar effects on T-cells specific for a NON-latent virus such as adenovirus, which is a major contributor to infection-related morbidity and mortality after alloHSCT. Thirty minutes of cycling exercise increased circulating adenovirus-specific T-cells 2.0-fold and augmented their ex vivo expansion by ~33% compared to rest without altering antigen and MHC-specific autologous target cell killing capabilities. We conclude that exercise is a simple and economical adjuvant to boost the isolation and manufacture of therapeutic VSTs specific to latent and non-latent viruses from healthy donors. Copyright © 2018. Published by Elsevier Inc.

  15. Dendritic cell based PSMA immunotherapy for prostate cancer using a CD40-targeted adenovirus vector.

    Directory of Open Access Journals (Sweden)

    Briana Jill Williams

    Full Text Available Human prostate tumor vaccine and gene therapy trials using ex vivo methods to prime dendritic cells (DCs with prostate specific membrane antigen (PSMA have been somewhat successful, but to date the lengthy ex vivo manipulation of DCs has limited the widespread clinical utility of this approach. Our goal was to improve upon cancer vaccination with tumor antigens by delivering PSMA via a CD40-targeted adenovirus vector directly to DCs as an efficient means for activation and antigen presentation to T-cells. To test this approach, we developed a mouse model of prostate cancer by generating clonal derivatives of the mouse RM-1 prostate cancer cell line expressing human PSMA (RM-1-PSMA cells. To maximize antigen presentation in target cells, both MHC class I and TAP protein expression was induced in RM-1 cells by transduction with an Ad vector expressing interferon-gamma (Ad5-IFNγ. Administering DCs infected ex vivo with CD40-targeted Ad5-huPSMA, as well as direct intraperitoneal injection of the vector, resulted in high levels of tumor-specific CTL responses against RM-1-PSMA cells pretreated with Ad5-IFNγ as target cells. CD40 targeting significantly improved the therapeutic antitumor efficacy of Ad5-huPSMA encoding PSMA when combined with Ad5-IFNγ in the RM-1-PSMA model. These results suggest that a CD-targeted adenovirus delivering PSMA may be effective clinically for prostate cancer immunotherapy.

  16. Comparison of Liver Detargeting Strategies for Systemic Therapy with Oncolytic Adenovirus Serotype 5

    Directory of Open Access Journals (Sweden)

    Tien V. Nguyen

    2017-08-01

    Full Text Available Oncolytic viruses would ideally be of use for systemic therapy to treat disseminated cancer. To do this safely, this may require multiple layers of cancer specificity. The pharmacology and specificity of oncolytic adenoviruses can be modified by (1 physical retargeting, (2 physical detargeting, (3 chemical shielding, or (4 by modifying the ability of viral early gene products to selectively activate in cancer versus normal cells. We explored the utility of these approaches with oncolytic adenovirus serotype 5 (Ad5 in immunocompetent Syrian hamsters bearing subcutaneous HaK tumors. After a single intravenous injection to reach the distant tumors, the physically hepatocyte-detargeted virus Ad5-hexon-BAP was more effective than conditionally replicating Ad5-dl1101/07 with mutations in its E1A protein. When these control or Ad5 treated animals were treated a second time by intratumoral injection, prior exposure to Ad5 did not affect tumor growth, suggesting that anti-Ad immunity neither prevented treatment nor amplified anti-tumor immune responses. Ad5-dl1101/07 was next chemically shielded with polyethylene glycol (PEG. While 5 kDa of PEG blunted pro-inflammatory IL-6 production induced by Ad5-dl1101/07, this shielding reduced Ad oncolytic activity.

  17. Aggregation of Adenovirus 2 in Source Water and Impacts on Disinfection by Chlorine

    Science.gov (United States)

    Cromeans, Theresa L.; Metcalfe, Maureen G.; Humphrey, Charles D.; Hill, Vincent R.

    2016-01-01

    It is generally accepted that viral particles in source water are likely to be found as aggregates attached to other particles. For this reason, it is important to investigate the disinfection efficacy of chlorine on aggregated viruses. A method to produce adenovirus particle aggregation was developed for this study. Negative stain electron microscopy was used to measure aggregation before and after addition of virus particles to surface water at different pH and specific conductance levels. The impact of aggregation on the efficacy of chlorine disinfection was also examined. Disinfection experiments with human adenovirus 2 (HAdV2) in source water were conducted using 0.2 mg/L free chlorine at 5 °C. Aggregation of HAdV2 in source water (≥3 aggregated particles) remained higher at higher specific conductance and pH levels. However, aggregation was highly variable, with the percentage of particles present in aggregates ranging from 43 to 71 %. Upon addition into source water, the aggregation percentage dropped dramatically. On average, chlorination CT values (chlorine concentration in mg/L × time in min) for 3-log10 inactivation of aggregated HAdV2 were up to three times higher than those for dispersed HAdV2, indicating that aggregation reduced the disinfection rate. This information can be used by water utilities and regulators to guide decision making regarding disinfection of viruses in water. PMID:26910058

  18. Attachment and Detachment Behavior of Human Adenovirus and Surrogates in Fine Granular Limestone Aquifer Material.

    Science.gov (United States)

    Stevenson, Margaret E; Sommer, Regina; Lindner, Gerhard; Farnleitner, Andreas H; Toze, Simon; Kirschner, Alexander K T; Blaschke, Alfred P; Sidhu, Jatinder P S

    2015-09-01

    The transport of human adenovirus, nanoparticles, and PRD1 and MS2 bacteriophages was tested in fine granular limestone aquifer material taken from a borehole at a managed aquifer recharge site in Adelaide, South Australia. Comparison of transport and removal of virus surrogates with the pathogenic virus is necessary to understand the differences between the virus and surrogate. Because experiments using pathogenic viruses cannot be done in the field, laboratory tests using flow-through soil columns were used. Results show that PRD1 is the most appropriate surrogate for adenovirus in an aquifer dominated by calcite material but not under high ionic strength or high pH conditions. It was also found that straining due to size and the charge of the colloid were not dominant removal mechanisms in this system. Implications of this study indicate that a certain surrogate may not represent a specific pathogen solely based on similar size, morphology, and/or surface charge. Moreover, if a particular surrogate is representative of a pathogen in one aquifer system, it may not be the most appropriate surrogate in another porous media system. This was apparent in the inferior performance of MS2 as a surrogate, which is commonly used in virus transport studies. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  19. Adenovirus type 2 endopeptidase: an unusual phosphoprotein enzyme matured by autocatalysis

    Energy Technology Data Exchange (ETDEWEB)

    Chatterjee, P.K.; Flint, S.J.

    1987-02-01

    A 19-kDa protein, present in low copy number in purified adenovirus type 2, has been characterized. Several criteria were used to establish that this protein is neither a degradation product of the known structural proteins of the virion nor a minor, unusually modified, form of protein VII. This 19-kDa protein, unlike other virion proteins, possesses alkali-resistant phosphoamino acids. Analysis by partial proteolysis indicated that it is related to a 23-kDa phosphoprotein present in H2ts-1 virions assembled in infected cells maintained at 39/sup 0/C. Affinity labeling with (/sup 3/H)diisopropyl fluorophosphate showed that the 19-kDa protein contains the active site for a serine protease. The authors, therefore, conclude that the 19-kDa protein is the active form of the adenovirus-encoded endopeptidase, defined by the H2ts-1 mutation, and is synthesized as a 23-kDa precursor that appears to mature by autocatalysis.

  20. Implementación de buenas prácticas de promoción de personal y su relación con la cultura y el compromiso con la organización

    Directory of Open Access Journals (Sweden)

    Merlin Patricia Grueso Hinestroza

    2010-04-01

    Full Text Available La investigación genera hipótesis y prueba un modelo de relaciones entre los valores de la cultura organizacional, la implementación de una buena práctica de promoción de personal desde la perspectiva de género y el compromiso con la organización. Para la prueba de hipótesis se emplea un modelo de ecuaciones estructurales y se realiza una encuesta a 425 empleados. Los resultados llevan a concluir que los valores distancia al poder y orientación de largo plazo predicen la implementación de buenas prácticas de promoción de personal; los componentes del compromiso con la organización no evidenciaron asociaciones significativas con la implementación de una buena práctica de promoción de personal. El estudio contribuye al desarrollo de un cuerpo de investigación en torno a las implicaciones de la perspectiva de género en las organizaciones.

  1. Análisis de la ira en pacientes con cardiopatía isquémica de la ciudad de Medellín (Colombia

    Directory of Open Access Journals (Sweden)

    Andrea Ochoa Ochoa

    2010-01-01

    Full Text Available Este estudio tuvo como objetivo comparar los niveles de ira, y sus componentes, entre un grupo de personas con Cardiopatía Isquémica y un grupo control sin esta enfermedad. Participaron 90 pacientes con cardiopatía isquémica de la Clínica Cardiovascular en Medellín y 78 controles sin la enfermedad. A cada uno de los participantes de les administró la prueba del STAXI- 2. Los resultados muestran que, comparado con los controles, los pacientes con cardiopatía isquémica tuvieron niveles significativamente más altos de ira como rasgo (p < 0.01, específicamente en la subescala reacción de ira (p < 0.05, y expresión interna de ira (p < 0.05. Los pacientes con cardiopatía isquémica experimentan más frecuentemente sentimientos de ira (ira rasgo, aparentemente debido a que son más sensibles a las críticas de los demás (reacción de ira, pero tienden a suprimir la expresión de esta emoción (expresión de ira interna. Estos datos confirman la necesidad de implementar programas dirigidos al manejo adecuado de la ira en estos pacientes y entender mejor las implicaciones que pueda tener la ira en la progresión de su enfermedad.

  2. Implicaciones a México a Bloques Comerciales en el Mercado Mundial del Espárrago

    OpenAIRE

    Ramón G. Guajardo Quiroga; Universidad Autónoma de Nuevo León, México

    2005-01-01

    Este artículo estudia el impacto de la formación de bloques comerciales en el comercio mundial del espárrago (NAFTA y TLCUEM). Se construyó un modelo de equilibrio espacial con precios endógenos y se diseñaron varios escenarios, de los cuales se presentan cuatro, con diversos grados de apertura comercial. Los resultados muestran que los productores mexicanos de espárragos resultan favorecidos con la integración de estos bloques. Sin embargo, en el escenario que modela la eliminación total de ...

  3. STAT2 Knockout Syrian Hamsters Support Enhanced Replication and Pathogenicity of Human Adenovirus, Revealing an Important Role of Type I Interferon Response in Viral Control.

    Directory of Open Access Journals (Sweden)

    Karoly Toth

    2015-08-01

    Full Text Available Human adenoviruses have been studied extensively in cell culture and have been a model for studies in molecular, cellular, and medical biology. However, much less is known about adenovirus replication and pathogenesis in vivo in a permissive host because of the lack of an adequate animal model. Presently, the most frequently used permissive immunocompetent animal model for human adenovirus infection is the Syrian hamster. Species C human adenoviruses replicate in these animals and cause pathology that is similar to that seen with humans. Here, we report findings with a new Syrian hamster strain in which the STAT2 gene was functionally knocked out by site-specific gene targeting. Adenovirus-infected STAT2 knockout hamsters demonstrated an accentuated pathology compared to the wild-type control animals, and the virus load in the organs of STAT2 knockout animals was 100- to 1000-fold higher than that in wild-type hamsters. Notably, the adaptive immune response to adenovirus is not adversely affected in STAT2 knockout hamsters, and surviving hamsters cleared the infection by 7 to 10 days post challenge. We show that the Type I interferon pathway is disrupted in these hamsters, revealing the critical role of interferon-stimulated genes in controlling adenovirus infection. This is the first study to report findings with a genetically modified Syrian hamster infected with a virus. Further, this is the first study to show that the Type I interferon pathway plays a role in inhibiting human adenovirus replication in a permissive animal model. Besides providing an insight into adenovirus infection in humans, our results are also interesting from the perspective of the animal model: STAT2 knockout Syrian hamster may also be an important animal model for studying other viral infections, including Ebola-, hanta-, and dengue viruses, where Type I interferon-mediated innate immunity prevents wild type hamsters from being effectively infected to be used as

  4. Tropism ablation and stealthing of oncolytic adenovirus enhances systemic delivery to tumors and improves virotherapy of cancer

    Czech Academy of Sciences Publication Activity Database

    Green, N. K.; Hale, A.; Cawood, R.; Illingworth, S.; Herbert, C.; Hermiston, T.; Šubr, Vladimír; Ulbrich, Karel; van Rooijen, N.; Seymour, L. W.; Fisher, K. D.

    2012-01-01

    Roč. 7, č. 11 (2012), s. 1683-1695 ISSN 1743-5889 R&D Projects: GA AV ČR IAAX00500803 Institutional research plan: CEZ:AV0Z40500505 Institutional support: RVO:61389013 Keywords : clodronate liposomes * polymer-coated adenovirus * predosing strategy Subject RIV: CD - Macromolecular Chemistry Impact factor: 5.260, year: 2012

  5. Adenovirus entry from the apical surface of polarized epithelia is facilitated by the host innate immune response.

    Directory of Open Access Journals (Sweden)

    Poornima L N Kotha

    2015-03-01

    Full Text Available Prevention of viral-induced respiratory disease begins with an understanding of the factors that increase or decrease susceptibility to viral infection. The primary receptor for most adenoviruses is the coxsackievirus and adenovirus receptor (CAR, a cell-cell adhesion protein normally localized at the basolateral surface of polarized epithelia and involved in neutrophil transepithelial migration. Recently, an alternate isoform of CAR, CAREx8, has been identified at the apical surface of polarized airway epithelia and is implicated in viral infection from the apical surface. We hypothesized that the endogenous role of CAREx8 may be to facilitate host innate immunity. We show that IL-8, a proinflammatory cytokine and a neutrophil chemoattractant, stimulates the protein expression and apical localization of CAREx8 via activation of AKT/S6K and inhibition of GSK3β. Apical CAREx8 tethers infiltrating neutrophils at the apical surface of a polarized epithelium. Moreover, neutrophils present on the apical-epithelial surface enhance adenovirus entry into the epithelium. These findings suggest that adenovirus evolved to co-opt an innate immune response pathway that stimulates the expression of its primary receptor, apical CAREx8, to allow the initial infection the intact epithelium. In addition, CAREx8 is a new target for the development of novel therapeutics for both respiratory inflammatory disease and adenoviral infection.

  6. Cyclophosphamide increases transgene expression mediated by an oncolytic adenovirus in glioma-bearing mice monitored by bioluminescence imaging

    NARCIS (Netherlands)

    Lamfers, Martine L. M.; Fulci, Giulia; Gianni, Davide; Tang, Yi; Kurozumi, Kazuhiko; Kaur, Balveen; Moeniralm, Sharif; Saeki, Yoshinaga; Carette, Jan E.; Weissleder, Ralph; Vandertop, W. Peter; van Beusechem, Victor W.; Dirven, Clemens M. F.; Chiocca, E. Antonio

    2006-01-01

    Approaches to improve the oncolytic potency of replication-competent adenoviruses include the insertion of therapeutic transgenes into the viral genome. Little is known about the levels and duration of in vivo transgene expression by cells infected with such "armed" viruses. Using a tumor-selective

  7. Traceless bioresponsive shielding of adenovirus hexon with HPMA copolymers maintains transduction capacity in vitro and in vivo

    Czech Academy of Sciences Publication Activity Database

    Prill, J.-M.; Šubr, Vladimír; Pasquarelli, N.; Engler, T.; Hoffmeister, A.; Kochanek, S.; Ulbrich, Karel; Kreppel, F.

    2014-01-01

    Roč. 9, č. 1 (2014), e82716_1-e82716_15 E-ISSN 1932-6203 Grant - others:AV ČR(CZ) AP0802 Program:Akademická prémie - Praemium Academiae Institutional support: RVO:61389013 Keywords : adenovirus * HPMA copolymers * bioresponsive shielding Subject RIV: CD - Macromolecular Chemistry Impact factor: 3.234, year: 2014

  8. Multiple Cross-Species Transmission Events of Human/nAdenoviruses (HAdV) during Hominine Evolution

    Czech Academy of Sciences Publication Activity Database

    Hoppe, E.; Pauly, M.; Gillespie, T. R.; Akoua-Koffi, C.; Hohmann, G.; Fruth, B.; Karhemere, S.; Madinda, N. F.; Mugisha, L.; Muyembe, J.-J.; Todd, A.; Petrželková, Klára Judita; Gray, M.; Robbins, M.; Bergl, R. A.; Wittig, R. M.; Zuberbühler, K.; Boesch, C.; Schubert, G.; Leendertz, F. H.; Ehlers, B.; Calvignac-Spencer, S.

    2015-01-01

    Roč. 32, č. 8 (2015), s. 2072-2084 ISSN 0737-4038 R&D Projects: GA ČR GA206/09/0927 Institutional support: RVO:60077344 Keywords : adenovirus * African great apes * zoonosis Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 13.649, year: 2015

  9. Potentiation of radiation therapy by the oncolytic adenovirus dl1520 (ONYX-015) in human malignant glioma xenografts.

    NARCIS (Netherlands)

    Geoerger, B; Grill, J; Opolon, P; Morizet, J; Aubert, G; Lecluse, Y; Beusechem-Kaptein, van V.W.; Gerritsen, W.R.; Kirn, DH; Vassal, G

    2003-01-01

    In spite of aggressive surgery, irradiation and/or chemotherapy, treatment of malignant gliomas remains a major challenge in adults and children due to high treatment failure. We have demonstrated significant cell lysis and antitumour activity of the E1B-55 kDa-gene-deleted adenovirus ONYX-015

  10. Adenovirus and Herpesvirus Diversity in Free-Ranging Great Apes in the Sangha Region of the Republic of Congo

    Science.gov (United States)

    Seimon, Tracie A.; Olson, Sarah H.; Lee, Kerry Jo; Rosen, Gail; Ondzie, Alain; Cameron, Kenneth; Reed, Patricia; Anthony, Simon J.; Joly, Damien O.; McAloose, Denise; Lipkin, W. Ian

    2015-01-01

    Infectious diseases have caused die-offs in both free-ranging gorillas and chimpanzees. Understanding pathogen diversity and disease ecology is therefore critical for conserving these endangered animals. To determine viral diversity in free-ranging, non-habituated gorillas and chimpanzees in the Republic of Congo, genetic testing was performed on great-ape fecal samples collected near Odzala-Kokoua National Park. Samples were analyzed to determine ape species, identify individuals in the population, and to test for the presence of herpesviruses, adenoviruses, poxviruses, bocaviruses, flaviviruses, paramyxoviruses, coronaviruses, filoviruses, and simian immunodeficiency virus (SIV). We identified 19 DNA viruses representing two viral families, Herpesviridae and Adenoviridae, of which three herpesviruses had not been previously described. Co-detections of multiple herpesviruses and/or adenoviruses were present in both gorillas and chimpanzees. Cytomegalovirus (CMV) and lymphocryptovirus (LCV) were found primarily in the context of co-association with each other and adenoviruses. Using viral discovery curves for herpesviruses and adenoviruses, the total viral richness in the sample population of gorillas and chimpanzees was estimated to be a minimum of 23 viruses, corresponding to a detection rate of 83%. These findings represent the first description of DNA viral diversity in feces from free-ranging gorillas and chimpanzees in or near the Odzala-Kokoua National Park and form a basis for understanding the types of viruses circulating among great apes in this region. PMID:25781992

  11. Adenovirus 2, Bordetella bronchiseptica, and Parainfluenza Molecular Diagnostic Assay Results in Puppies After vaccination with Modified Live Vaccines.

    Science.gov (United States)

    Ruch-Gallie, R; Moroff, S; Lappin, M R

    2016-01-01

    Canine adenovirus 2, parainfluenza, and Bordetella bronchiseptica cause respiratory disease in dogs, and each has a modified live intranasal vaccine available. Molecular diagnostic assays to amplify specific nucleic acids are available for each of these agents. If positive molecular diagnostic assay results are common after vaccination, the positive predictive value of the diagnostic assays for disease would be decreased. To determine the impact of administration of commercially available modified live topical adenovirus 2, B. bronchiseptica, and parainfluenza vaccine has on the results of a commercially available PCR panel. Eight puppies from a research breeding facility negative for these pathogens. Blinded prospective pilot study. Puppies were vaccinated with a single dose of modified live topical adenovirus 2, B. bronchiseptica, and parainfluenza and parenteral dose of adenovirus 2, canine distemper virus, and parvovirus. Nasal and pharyngeal swabs were collected on multiple days and submitted for PCR assay. Nucleic acids of all 3 organisms contained in the topical vaccine were detected from both samples multiple times through 28 days after vaccination with higher numbers of positive samples detected between days 3 and 10 after vaccination. Vaccine status should be considered when interpreting respiratory agent PCR results if modified live vaccines have been used. Development of quantitative PCR and wild-type sequencing are necessary to improve positive predictive value of these assays by distinguishing vaccinate from natural infection. Copyright © 2015 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.

  12. Molecular characterization, phylogeny analysis and pathogenicity of a Muscovy duck adenovirus strain isolated in China in 2014

    Science.gov (United States)

    This study aimed to characterize a novel adenovirus (AdV) isolated from diseased Muscovy ducks in China. After the AdV was successfully propagated in duck embryo fibroblasts, the morphological and physicochemical properties of the virions were studied by electron microscopy and different tests. The ...

  13. El documental participativo como proceso educativo: hacia la realización de proyectos audiovisuales con enfoque intercultural

    Directory of Open Access Journals (Sweden)

    Juan Carlos A. Sandoval Rivera

    2015-01-01

    Full Text Available El objetivo del artículo es reflexionar sobre las implicaciones de una experiencia de creación documental que se realizó con jóvenes indígenas del sur de Veracruz. Durante este proyecto, a través de un proceso participativo, se produjo un documental sobre las prácticas de pesca tradicional que realizan las mujeres de la comunidad. A partir de esta experiencia y del material producido, se analiza el proceso educativo en la participación durante la producción audiovisual, donde se fortalece la identidad indígena local a través del uso de nuevas tecnologías y de métodos inclusivos que permiten la reflexión colectiva respecto a la importancia de saberes ancestrales relacionados con el manejo de territorio. El enfoque intercultural desde el cual se desarrolla el análisis, permite respondernos preguntas respecto a la producción de materiales audiovisuales en contextos de diversidad cultural; también, sobre temáticas relacionadas con el conocimiento indígena y el uso del territorio desde la cosmovisión local, así como de procesos de aprendizaje significativo.

  14. preescolares desnutridos con madres con obesidad y sin obesidad

    Directory of Open Access Journals (Sweden)

    Viridiana Vanessa Conzuelo-González

    2009-01-01

    Full Text Available El primer objetivo fue conocer cuántos menores de cinco años con diferentes grados de desnutrición tienen una madre con sobrepeso/obesidad/ en una comunidad indígena que vive en extrema pobreza y bajo condiciones de migración masculina internacional. El segundo fue comparar tres variables socionutricionales (ingreso familiar, educación de la madre y adecuación nutrimental de la dieta diaria entre estos hogares y los hogares con desnutrición infantil y madres sin obesidad. Se realizó un estudio transversal (2006-2007, en la comunidad mazahua de San Francisco Tepeolulco, Municipio de Temascalcingo; que incluyó a 85 hogares integrados por preescolares con desnutrición inscritos al programa Oportunidades. Se determinó el estado nutrición de los preescolares con indicadores antropométricos y se obtuvo el IMC de las madres de estos infantes. Se aplicó una encuesta socionutricional, incluida el recordatorio de 24 horas, y complementado con la observación participante (cualitativa. Se encontró que 83% de las madres mazahuas presentaron sobrepeso u obesidad. El estado de nutrición de los preescolares con madres con obesidad presentó un porcentaje mayor de desnutrición (76%. En la variable género, se encontró que 54% de los niños con madres con obesidad tenía baja talla. Al relacionar el nivel educativo de la madre, esta variable resultó ser estadísticamente significativa (p=0.015, donde el analfabetismo está más relacionado con la desnutrición infantil que tienen madres de bajo y/o peso normal. La elevada prevalencia de hogares conformados con preescolares con desnutrición y madres con obesidad, es un síntoma más de la pobreza en zonas indígenas en México, con bajo índice de desarrollo humano.

  15. Evidence of molecular evolution driven by recombination events influencing tropism in a novel human adenovirus that causes epidemic keratoconjunctivitis.

    Directory of Open Access Journals (Sweden)

    Michael P Walsh

    2009-06-01

    Full Text Available In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53 was isolated from an outbreak of epidemic keratoconjunctititis (EKC, a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D have been described. The complete genome of HAdV-D53 was sequenced to elucidate recombination events that lead to the emergence of a viable and highly virulent virus with a modified tropism. Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including the fiber gene encoding the primary cellular receptor binding site, HAdV-D22, (the epsilon determinant of the hexon gene, HAdV-D37 (including the penton base gene encoding the secondary cellular receptor binding site, and at least one unknown or unsequenced HAdV-D strain. Bootscanning analysis of the complete genomic sequence of this novel adenovirus, which we have re-named HAdV-D53, indicated at least five recombination events between the aforementioned adenoviruses. Intrahexon recombination sites perfectly framed the epsilon neutralization determinant that was almost identical to the HAdV-D22 prototype. Additional bootscan analysis of all HAdV-D hexon genes revealed recombinations in identical locations in several other adenoviruses. In addition, HAdV-D53 but not HAdV-D22 induced corneal inflammation in a mouse model. Serological analysis confirmed previous results and demonstrated that HAdV-D53 has a neutralization profile representative of the epsilon determinant of its hexon (HAdV-D22 and the fiber (HAdV-D8 proteins. Our recombinant hexon sequence is almost identical to the hexon sequences of the HAdV-D strain causing EKC outbreaks in Japan, suggesting that HAdV-D53 is pandemic as an emerging EKC agent. This documents

  16. A novel psittacine adenovirus identified during an outbreak of avian chlamydiosis and human psittacosis: zoonosis associated with virus-bacterium coinfection in birds.

    Directory of Open Access Journals (Sweden)

    Kelvin K W To

    2014-12-01

    Full Text Available Chlamydophila psittaci is found worldwide, but is particularly common among psittacine birds in tropical and subtropical regions. While investigating a human psittacosis outbreak that was associated with avian chlamydiosis in Hong Kong, we identified a novel adenovirus in epidemiologically linked Mealy Parrots, which was not present in healthy birds unrelated to the outbreak or in other animals. The novel adenovirus (tentatively named Psittacine adenovirus HKU1 was most closely related to Duck adenovirus A in the Atadenovirus genus. Sequencing showed that the Psittacine adenovirus HKU1 genome consists of 31,735 nucleotides. Comparative genome analysis showed that the Psittacine adenovirus HKU1 genome contains 23 open reading frames (ORFs with sequence similarity to known adenoviral genes, and six additional ORFs at the 3' end of the genome. Similar to Duck adenovirus A, the novel adenovirus lacks LH1, LH2 and LH3, which distinguishes it from other viruses in the Atadenovirus genus. Notably, fiber-2 protein, which is present in Aviadenovirus but not Atadenovirus, is also present in Psittacine adenovirus HKU1. Psittacine adenovirus HKU1 had pairwise amino acid sequence identities of 50.3-54.0% for the DNA polymerase, 64.6-70.7% for the penton protein, and 66.1-74.0% for the hexon protein with other Atadenovirus. The C. psittaci bacterial load was positively correlated with adenovirus viral load in the lung. Immunostaining for fiber protein expression was positive in lung and liver tissue cells of affected parrots, confirming active viral replication. No other viruses were found. This is the first documentation of an adenovirus-C. psittaci co-infection in an avian species that was associated with a human outbreak of psittacosis. Viral-bacterial co-infection often increases disease severity in both humans and animals. The role of viral-bacterial co-infection in animal-to-human transmission of infectious agents has not received sufficient attention

  17. A novel psittacine adenovirus identified during an outbreak of avian chlamydiosis and human psittacosis: zoonosis associated with virus-bacterium coinfection in birds.

    Science.gov (United States)

    To, Kelvin K W; Tse, Herman; Chan, Wan-Mui; Choi, Garnet K Y; Zhang, Anna J X; Sridhar, Siddharth; Wong, Sally C Y; Chan, Jasper F W; Chan, Andy S F; Woo, Patrick C Y; Lau, Susanna K P; Lo, Janice Y C; Chan, Kwok-Hung; Cheng, Vincent C C; Yuen, Kwok-Yung

    2014-12-01

    Chlamydophila psittaci is found worldwide, but is particularly common among psittacine birds in tropical and subtropical regions. While investigating a human psittacosis outbreak that was associated with avian chlamydiosis in Hong Kong, we identified a novel adenovirus in epidemiologically linked Mealy Parrots, which was not present in healthy birds unrelated to the outbreak or in other animals. The novel adenovirus (tentatively named Psittacine adenovirus HKU1) was most closely related to Duck adenovirus A in the Atadenovirus genus. Sequencing showed that the Psittacine adenovirus HKU1 genome consists of 31,735 nucleotides. Comparative genome analysis showed that the Psittacine adenovirus HKU1 genome contains 23 open reading frames (ORFs) with sequence similarity to known adenoviral genes, and six additional ORFs at the 3' end of the genome. Similar to Duck adenovirus A, the novel adenovirus lacks LH1, LH2 and LH3, which distinguishes it from other viruses in the Atadenovirus genus. Notably, fiber-2 protein, which is present in Aviadenovirus but not Atadenovirus, is also present in Psittacine adenovirus HKU1. Psittacine adenovirus HKU1 had pairwise amino acid sequence identities of 50.3-54.0% for the DNA polymerase, 64.6-70.7% for the penton protein, and 66.1-74.0% for the hexon protein with other Atadenovirus. The C. psittaci bacterial load was positively correlated with adenovirus viral load in the lung. Immunostaining for fiber protein expression was positive in lung and liver tissue cells of affected parrots, confirming active viral replication. No other viruses were found. This is the first documentation of an adenovirus-C. psittaci co-infection in an avian species that was associated with a human outbreak of psittacosis. Viral-bacterial co-infection often increases disease severity in both humans and animals. The role of viral-bacterial co-infection in animal-to-human transmission of infectious agents has not received sufficient attention and should be

  18. Link of the unique oncogenic properties of adenovirus type 9 E4-ORF1 to a select interaction with the candidate tumor suppressor protein ZO-2

    OpenAIRE

    Glaunsinger, Britt A.; Weiss, Robert S.; Lee, Siu Sylvia; Javier, Ronald

    2001-01-01

    Adenovirus type 9 (Ad9) is distinct among human adenoviruses because it elicits solely mammary tumors in animals and its primary oncogenic determinant is the E4 region-encoded ORF1 (E4-ORF1) protein. We report here that the PDZ domain-containing protein ZO-2, which is a candidate tumor suppressor protein, is a cellular target for tumorigenic Ad9 E4-ORF1 but not for non-tumorigenic wild-type E4-ORF1 proteins encoded by adenovirus types 5 and 12. Complex formation was mediated by the C-terminal...

  19. Transduction of skin-migrating dendritic cells by human adenovirus 5 occurs via an actin-dependent phagocytic pathway.

    Science.gov (United States)

    Guzman, Efrain; Taylor, Geraldine; Hope, Jayne; Herbert, Rebecca; Cubillos-Zapata, Carolina; Charleston, Bryan

    2016-10-01

    Dendritic cells (DC) are central to the initiation of immune responses, and various approaches have been used to target vaccines to DC in order to improve immunogenicity. Cannulation of lymphatic vessels allows for the collection of DC that migrate from the skin. These migrating DC are involved in antigen uptake and presentation following vaccination. Human replication-deficient adenovirus (AdV) 5 is a promising vaccine vector for delivery of recombinant antigens. Although the mechanism of AdV attachment and penetration has been extensively studied in permissive cell lines, few studies have addressed the interaction of AdV with DC. In this study, we investigated the interaction of bovine skin-migrating DC and replication-deficient AdV-based vaccine vectors. We found that, despite lack of expression of Coxsackie B-Adenovirus Receptor and other known adenovirus receptors, AdV readily enters skin-draining DC via an actin-dependent endocytosis. Virus exit from endosomes was pH independent, and neutralizing antibodies did not prevent virus entry but did prevent virus translocation to the nucleus. We also show that combining adenovirus with adjuvant increases the absolute number of intracellular virus particles per DC but not the number of DC containing intracellular virus. This results in increased trans-gene expression and antigen presentation. We propose that, in the absence of Coxsackie B-Adenovirus Receptor and other known receptors, AdV5-based vectors enter skin-migrating DC using actin-dependent endocytosis which occurs in skin-migrating DC, and its relevance to vaccination strategies and vaccine vector targeting is discussed.

  20. Inactivation of Adenovirus Type 5, Rotavirus WA and Male Specific Coliphage (MS2 in Biosolids by Lime Stabilization

    Directory of Open Access Journals (Sweden)

    Aaron B. Margolin

    2007-03-01

    Full Text Available The use of lime to reduce or eliminate pathogen content is a cost-effective treatment currently employed in many Class B biosolids production plants in the United States. A bench scale model of lime stabilization was designed to evaluate the survival of adenovirus type 5, rotavirus Wa, and the male specific bacteriophage, MS2, in various matrices. Each virus was initially evaluated independently in a reverse osmosis treated water matrix limed with an aqueous solution of calcium hydroxide for 24-hr at 22 ± 5°C. In all R/O water trials, adenovirus type 5, rotavirus Wa and MS2 were below detectable levels (<100.5 TCID50/mL and <1 PFU/mL respectively following 0.1-hr of liming. Adenovirus type 5, rotavirus Wa, and MS2, were inoculated into composted, raw and previously limed matrices, representative of sludge and biosolids, to achieve a final concentration of approximately 104 PFU or TCID50/mL. Each matrix was limed for 24-hr at 22 ± 5°C and 4 ± 2°C. In all trials virus was below detectable levels following a 24-hr incubation. The time required for viral inactivation varied depending on the temperature and sample matrix. This research demonstrates reduction of adenovirus type 5, rotavirus Wa, and male-specific bacteriophage, in water, sludge and biosolids matrices following addition of an 8% calcium hydroxide slurry to achieve a pH of 12 for 2-hr reduced to 11.5 for 22-hr by addition of 0.1 N HCl. In these trials, MS2 was a conservative indicator of the efficacy of lime stabilization of adenovirus Type 5 and rotavirus Wa and therefore is proposed as a useful indicator organism.

  1. autorregulado con estudiantes universitarios

    Directory of Open Access Journals (Sweden)

    Jairo Andrés Montes

    2005-01-01

    Full Text Available El propósito del presente estudio es describir la forma en la que se presentan los procesos de aprendizaje autorregulado con un grupo de estudiantes (22 estudiantes de tercer semestre de Psicología de la PUJ, Cali, en el evento de preparación para la presentación un examen. Asimismo se describen las correlaciones que ocurren entre las distintas fases de dicho proceso de autorregulación del aprendizaje. Para conseguir los objetivos propuestos se ha hecho uso de una observación de desempeño en tiempo real, es decir, de la observación durante una sesión de preparación de examen de los estudiantes, en la cual se emplearon protocolos verbales para dar cuenta de lo que «pasaba por su mente» mientras estudiaban. Una entrevista semi-estructurada y una prueba objetiva. Los resultados fueron analizados a la luz del modelo mixto de procesamiento de información y constructivismo abordado por Winne(1998. Como resultado se encontró una relación significativa entre los niveles de desempeño en el proceso de ARR y el resultado del examen. Igualmente se encontraron bajos niveles de regulación en una parte importante de la muestra y un desfase significativo entre conocimiento declarativo de ARR y desempeño en el mismo

  2. A human type 5 adenovirus-based tuberculosis vaccine induces robust T cell responses in humans despite preexisting anti-adenovirus immunity.

    Science.gov (United States)

    Smaill, Fiona; Jeyanathan, Mangalakumari; Smieja, Marek; Medina, Maria Fe; Thanthrige-Don, Niroshan; Zganiacz, Anna; Yin, Cindy; Heriazon, Armando; Damjanovic, Daniela; Puri, Laura; Hamid, Jemila; Xie, Feng; Foley, Ronan; Bramson, Jonathan; Gauldie, Jack; Xing, Zhou

    2013-10-02

    There is an urgent need to develop new tuberculosis (TB) vaccines to safely and effectively boost Bacille Calmette-Guérin (BCG)-triggered T cell immunity in humans. AdHu5Ag85A is a recombinant human type 5 adenovirus (AdHu5)-based TB vaccine with demonstrated efficacy in a number of animal species, yet it remains to be translated to human applications. In this phase 1 study, we evaluated the safety and immunogenicity of AdHu5Ag85A in both BCG-naïve and previously BCG-immunized healthy adults. Intramuscular immunization of AdHu5Ag85A was safe and well tolerated in both trial volunteer groups. Moreover, although AdHu5Ag85A was immunogenic in both trial volunteer groups, it much more potently boosted polyfunctional CD4(+) and CD8(+) T cell immunity in previously BCG-vaccinated volunteers. Furthermore, despite prevalent preexisting anti-AdHu5 humoral immunity in most of the trial volunteers, we found little evidence that such preexisting anti-AdHu5 immunity significantly dampened the potency of AdHu5Ag85A vaccine. This study supports further clinical investigations of the AdHu5Ag85A vaccine for human applications. It also suggests that the widely perceived negative effect of preexisting anti-AdHu5 immunity may not be universally applied to all AdHu5-based vaccines against different types of human pathogens.

  3. Suppression of leaky expression of adenovirus genes by insertion of microRNA-targeted sequences in the replication-incompetent adenovirus vector genome

    Directory of Open Access Journals (Sweden)

    Kahori Shimizu

    2014-01-01

    Full Text Available Leaky expression of adenovirus (Ad genes occurs following transduction with a conventional replication-incompetent Ad vector, leading to an induction of cellular immunity against Ad proteins and Ad protein-induced toxicity, especially in the late phase following administration. To suppress the leaky expression of Ad genes, we developed novel Ad vectors by incorporating four tandem copies of sequences with perfect complementarity to miR-122a or miR-142-3p into the 3′-untranslated region (UTR of the E2A, E4, or pIX gene, which were mainly expressed from the Ad vector genome after transduction. These Ad vectors easily grew to high titers comparable to those of a conventional Ad vector in conventional 293 cells. The leaky expression of these Ad genes in mouse organs was significantly suppressed by 2- to 100-fold, compared with a conventional Ad vector, by insertion of the miRNA-targeted sequences. Notably, the Ad vector carrying the miR-122a–targeted sequences into the 3′-UTR of the E4 gene expressed higher and longer-term transgene expression and more than 20-fold lower levels of all the Ad early and late genes examined in the liver than a conventional Ad vector. miR-122a–mediated suppression of the E4 gene expression in the liver significantly reduced the hepatotoxicity which an Ad vector causes via both adaptive and non-adaptive immune responses.

  4. Adenovirus 4/7 Vaccine's Effect on Disease Rates Is Associated With Disappearance of Adenovirus on Building Surfaces at a Military Recruit Base.

    Science.gov (United States)

    Broderick, Michael; Myers, Christopher; Balansay, Melinda; Vo, Scott; Osuna, Angel; Russell, Kevin

    2017-11-01

    Febrile respiratory illness resulting from adenovirus types 4 and 7 (Ad4/7) was endemic at military training camps, but controlled by an Ad4/7 vaccine from the 1970s to 1999, the year it was discontinued. Thereafter, rates returned to prevaccine levels. Rates dropped after reintroduction of an Ad4/7 vaccine in 2011. Surfaces of the barracks and medical clinic of a training camp were swabbed in 3 studies in 2004 and 1 study in 2007, and tested with culture and polymerase chain reaction (PCR). Similar swabbing was done in 2013 and 2015 and tested with PCR. In the studies before 2011 (prevaccine), 12% of samples were Ad4/7 positive by culture and 27% positive by PCR. In the 2 studies after 2011 (postvaccine), no samples were Ad4/7 positive. The Ad 4/7 vaccine has resulted in the near elimination of Ad4/7-related disease and the disappearance of Ad4/7 from surfaces in a military basic training camp. Renewed transmission of Ad4/7 in this setting would likely require new importation from military recruits and an immunologically naive cohort, which the current vaccination program prevents. Reprint & Copyright © 2017 Association of Military Surgeons of the U.S.

  5. An adenovirus vectored mucosal adjuvant augments protection of mice immunized intranasally with an adenovirus-vectored foot-and-mouth disease virus subunit vaccine.

    Science.gov (United States)

    Alejo, Diana M; Moraes, Mauro P; Liao, Xiaofen; Dias, Camila C; Tulman, Edan R; Diaz-San Segundo, Fayna; Rood, Debra; Grubman, Marvin J; Silbart, Lawrence K

    2013-04-26

    Foot-and-mouth disease virus (FMDV) is a highly contagious pathogen that causes severe morbidity and economic losses to the livestock industry in many countries. The oral and respiratory mucosae are the main ports of entry of FMDV, so the stimulation of local immunity in these tissues may help prevent initial infection and viral spread. E. coli heat-labile enterotoxin (LT) has been described as one of the few molecules that have adjuvant activity at mucosal surfaces. The objective of this study was to evaluate the efficacy of replication-defective adenovirus 5 (Ad5) vectors encoding either of two LT-based mucosal adjuvants, LTB or LTR72. These vectored adjuvants were delivered intranasally to mice concurrent with an Ad5-FMDV vaccine (Ad5-A24) to assess their ability to augment mucosal and systemic humoral immune responses to Ad5-A24 and protection against FMDV. Mice receiving Ad5-A24 plus Ad5-LTR72 had higher levels of mucosal and systemic neutralizing antibodies than those receiving Ad5-A24 alone or Ad5-A24 plus Ad5-LTB. The vaccine plus Ad5-LTR72 group also demonstrated 100% survival after intradermal challenge with a lethal dose of homologous FMDV serotype A24. These results suggest that Ad5-LTR72 could be used as an important tool to enhance mucosal and systemic immunity against FMDV and potentially other pathogens with a common route of entry. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. Ética profesional: errores e implicaciones en la consulta y utilización de la información científico técnica Professional ethics: mistakes and implications in the outpatient department and use of the scientific and technical information

    Directory of Open Access Journals (Sweden)

    Esther Mayor Guerra

    2012-01-01

    Full Text Available La actividad que precisa la generación de conocimientos para la producción científica, implica un reto diario para el profesional de la información. En este artículo se realiza un acercamiento a la labor, la responsabilidad y el desempeño de este último ante la magnitud de implicaciones éticas y errores cometidos por los autores en sus escritos (muchos de ellos evitables, particularmente con respecto a la consulta y utilización de documentos publicados sobre la medicina y ramas afines.The activity which demands of knowledge for the scientific production, implies a daily challenge for the information professional. In this article an approach to the professional's work, responsibility and performance is presented, taking into account the magnitude of ethical implications and mistakes committed by the authors in their writings (many of them avoidable, particularly with regard to the outpatient department and the use of published documents on medicine and related branches.

  7. Terapia con células madre en cirrosis = Stem cell therapy in cirrhosis

    Directory of Open Access Journals (Sweden)

    Londoño Agudelo, Jessica María

    2011-12-01

    Full Text Available En este artículo se presenta una revisión de la literatura sobre las células madre como terapia para la cirrosis hepática, con énfasis en describir la situación científica actual y las implicaciones clínicas de los avances. Se ha propuesto la terapia celular en cirrosis por la escasez de donantes de hígado y las complicaciones derivadas de la terapia inmunosupresora. Las células madre se pueden obtener de diferentes fuentes como el blastocisto, la médula ósea e incluso hepatocitos maduros; pero la capacidad de proliferación y diferenciación de cada uno de estos tipos celulares es distinta y, entre otras consideraciones, es lo que va a definir su utilidad clínica final. En la literatura se encuentran varios estudios en modelos animales y en seres humanos que evalúan la seguridad y factibilidad de esta técnica en cuanto a la mejoría de la fibrosis, la capacidad de diferenciación y replicación celulares y algunos parámetros clínicos. Los resultados, aunque alentadores, se deben interpretar con cuidado. En conclusión, antes de aceptar esta modalidad terapéutica se requieren estudios de mejor calidad y con homogeneidad metodológica porque su uso aún es controversial.

  8. Puentes con vigas pretensadas

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1965-07-01

    Full Text Available This paper describes one of the three bridges which Hidrocivil, S. A., has built in Catalonia (northern Spain, over the river Ripoll. The other two bridges are very similar to this one, both in construction and design, and show only minor adjustments to the local topography. The contracting firm proposed several alterations in the prefabrication and constructional procedure, in relation to the initial project, and these changes were accepted. The main feature of these projects is the use of prestressed beams, built at the workshop in sections, and joined together by means of sixty 7 mm cables in each beam. As the shear forces are more acute at the joints, the end of each section has a kind of diaphragm, to provide a large contact area, and hence greater surface to transmit the shear forces. The methods of construction are also of interest. Briefly, they involve building the bridge piles, and use these to support a provisional structure with transversal movement. This provisional structure, in turn, served as platform for two bridge cranes, which lifted the girders to their final location. After the first span was completed, the deck was concreted and the auxiliary structure pushed forward to the next span, to repeat the same operations. This arrangement saved the use of provisional framework.En este trabajo se describe uno de los tres puentes que Hidrocivil, S. A., ha construido.—previo concurso— en la región catalana; concretamente, el que salva el río Ripoll. Los otros dos no han sido objeto de descripción general por ser muy similares, en lo que a ejecución y concepción se refiere, con la única variante que presentan las características topográficas locales. La empresa propuso ciertas variantes— que fueron aceptadas— en la prefabricación y métodos de construcción. El interés de estas obras se centra en el empleo de vigas pretensadas, prefabricadas en taller por trozos, y solidarizados en el mismo mediante las operaciones

  9. Violencia con el anciano

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    Rita Campillo Motilva

    2002-08-01

    Full Text Available La violencia doméstica es tan antigua como la humanidad misma y se reconocen la violencia infantil, contra la mujer y al anciano, fundamentalmente; siendo este último grupo una población en ascenso por las mayores expectativas de vida de los últimos años. Como resultado de ello, el número de casos de abuso en el anciano se incrementará y el impacto de este abuso sobre la salud debe ser considerado de forma adecuada. La gama de maltratos es variadísima e incluye el abuso físico, emocional, financiero, sexual, por negligencia, negación a brindarle ayuda y otras formas más. Los ancianos con deterioro cognitivo son los más vulnerables. El médico en la atención primaria de salud es un pilar importante en la prevención y educación de este problema.Domestic violence is as old as humanity itself. Child, women and elderly abuse are mainly recognized. The elderly group is increasing due to the higher life expectancy experimented during the last years. As a result, the number of battered elderly will grow and the impact of this abuse on health should be adequately considered. The range of abuse is very wide and it includes physical, emotional, financial and sexual abuse, negligence, rejection to give assistance and others. The elderly with cognitive deterioration are the most vulnerable. The physician at the primary health care level is an important milestone in the prevention and education of this problem.

  10. Oral or parenteral administration of replication-deficient adenoviruses expressing the measles virus haemagglutinin and fusion proteins: protective immune responses in rodents.

    Science.gov (United States)

    Fooks, A R; Jeevarajah, D; Lee, J; Warnes, A; Niewiesk, S; ter Meulen, V; Stephenson, J R; Clegg, J C

    1998-05-01

    The genes encoding the measles virus (MV) haemagglutinin (H) and fusion (F) proteins were placed under the control of the human cytomegalovirus immediate early promoter in a replication-deficient adenovirus vector. Immunofluorescence and radioimmune precipitation demonstrated the synthesis of each protein and biological activity was confirmed by the detection of haemadsorption and fusion activities in infected cells. Oral as well as parenteral administration of the H-expressing recombinant adenovirus elicited a significant protective response in mice challenged with MV. While the F-expressing adenovirus failed to protect mice, cotton rats immunized with either the H- or F-expressing recombinant showed reduced MV replication in the lungs. Antibodies elicited in mice following immunization with either recombinant had no in vitro neutralizing activity, suggesting a protective mechanism involving a cell-mediated immune response. This study demonstrates the feasibility of using oral administration of adenovirus recombinants to induce protective responses to heterologous proteins.

  11. Differential effects of hydroxyurea on the survival of UV- and MNNG-treated adenovirus 5

    International Nuclear Information System (INIS)

    Day, R.S. III; Ziolkowski, C.H.J.

    1982-01-01

    The effects of hydroxyurea on plaque formation by UV-irradiated and MNNG-treated adenovirus 5 were investigated. Hydroxyurea blocked the recovery of UV-irradiated viruses in all cases studied, but the effect was less when fibroblasts from a patient with xeroderma pigmentosum were used. This fact supports the notion that hydroxyurea blocks excision repair of UV-produced damage. The recovery of MNNG-treated viruses was not blocked by hydroxyurea when viruses were used to infect normal human fibroblasts, but was blocked if the cell strain used as viral host were deficient in repair of O 6 -methylguanine. To account for these data, we propose that hydroxyurea blocks repair in which DNA polymerases play a role, but does not block repair in which DNA polymerases are not required. (orig.)

  12. Differential effects of hydroxyurea on the survival of UV- and MNNG-treated adenovirus 5

    Energy Technology Data Exchange (ETDEWEB)

    Day, R.S. III; Ziolkowski, C.H.J. (National Inst. for Cancer Research, Bethesda, MD (USA). Nucleic Acid Section)

    1982-01-01

    The effects of hydroxyurea on plaque formation by UV-irradiated and MNNG-treated adenovirus 5 were investigated. Hydroxyurea blocked the recovery of UV-irradiated viruses in all cases studied, but the effect was less when fibroblasts from a patient with xeroderma pigmentosum were used. This fact supports the notion that hydroxyurea blocks excision repair of UV-produced damage. The recovery of MNNG-treated viruses was not blocked by hydroxyurea when viruses were used to infect normal human fibroblasts, but was blocked if the cell strain used as viral host were deficient in repair of O/sup 6/-methylguanine. To account for these data, we propose that hydroxyurea blocks repair in which DNA polymerases play a role, but does not block repair in which DNA polymerases are not required.

  13. Immunology and evolvement of the adenovirus prime, MVA boost Ebola virus vaccine.

    Science.gov (United States)

    Zhou, Yan; Sullivan, Nancy J

    2015-08-01

    The 2014 Ebola virus outbreak caused an order of magnitude more deaths in a single outbreak than all previous known outbreaks combined, affecting both local and international public health, and threatening the security and economic stability of the countries in West Africa directly confronting the outbreak. The severity of the epidemic lead to a global response to assist with patient care, outbreak control, and deployment of vaccines. The latter was possible due to the long history of basic and clinical research aimed at identifying a safe and effective vaccine to protect against Ebola virus infection. This review highlights the immunology, development, and progress of vaccines based on replication-defective adenovirus vectors, culminating in the successful launch of the first Phase III trial of an Ebola virus vaccine. Published by Elsevier Ltd.

  14. Increased suppression of oncolytic adenovirus carrying mutant k5 on colorectal tumor

    International Nuclear Information System (INIS)

    Fan Junkai; Xiao Tian; Gu Jinfa; Wei Na; He Lingfeng; Ding Miao; Liu Xinyuan

    2008-01-01

    Angiogenesis plays a key role in the development of a wide variety of malignant tumors. The approach of targeting antiangiogenesis has become an important field of cancer gene therapy. In this study, the antiangiogenesis protein K5 (the kringle 5 of human plasminogen) has been mutated by changing leucine71 to arginine to form mK5. Then the ZD55-mK5, which is an oncolytic adenovirus expressing mK5, was constructed. It showed stronger inhibition on proliferation of human umbilical vein endothelial cell. Moreover, in tube formation and embryonic chorioallantoic membrane assay, ZD55-mK5 exhibited more effective antiangiogenesis than ZD55-K5. In addition, ZD55-mK5 generated obvious suppression on the growth of colorectal tumor xenografts and prolonged the life span of nude mice. These results indicate that ZD55-mK5 is a potent agent for inhibiting the tumor angiogenesis and tumor growth

  15. Systemic adenovirus infection in Bearded Dragons (Pogona vitticeps): histological, ultrastructural and molecular findings.

    Science.gov (United States)

    Moormann, S; Seehusen, F; Reckling, D; Kilwinski, J; Puff, C; Elhensheri, M; Wohlsein, P; Peters, M

    2009-07-01

    Three Inland Bearded Dragons (Pogona vitticeps) from two breeding groups were humanely destroyed following a period of anorexia. Two of the animals were 8-months old and related and one animal was approximately 2-weeks old. Necropsy examination revealed poor bodily condition but no other gross abnormalities. Microscopically there was non-suppurative hepatitis and interstitial nephritis. Multiple large, amphophilic, intranuclear inclusion bodies were present within hepatocytes and epithelial cells of the bile ducts, renal tubules, small and large intestinal mucosa, pancreatic acini and oral mucous membranes. Transmission electron microscopy (TEM) demonstrated that the inclusions comprised viral particles with morphology consistent with an adenovirus. A fragment of the adenoviral polymerase gene was amplified, sequenced and compared with other reptilian adenoviral sequences.

  16. The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding

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    E. Gout

    2010-01-01

    Full Text Available Human type 3 adenovirus dodecahedron (a virus like particle made of twelve penton bases features the ability to enter cells through Heparan Sulphate Proteoglycans (HSPGs and integrins interaction and is used as a versatile vector to deliver DNA or proteins. Cryo-EM reconstruction of the pseudoviral particle with Heparan Sulphate (HS oligosaccharide shows an extradensity on the RGD loop. A set of mutants was designed to study the respective roles of the RGD sequence (RGE mutant and of a basic sequence located just downstream. Results showed that the RGE mutant binding to the HS deficient CHO-2241 cells was abolished and unexpectedly, mutation of the basic sequence (KQKR to AQAS dramatically decreased integrin recognition by the viral pseudoparticle. This basic sequence is thus involved in integrin docking, showing a close interplay between HSPGs and integrin receptors.

  17. Characterization of the Antiglioma Effect of the Oncolytic Adenovirus VCN-01.

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    Beatriz Vera

    Full Text Available Despite the recent advances in the development of antitumor therapies, the prognosis for patients with malignant gliomas remains dismal. Therapy with tumor-selective viruses is emerging as a treatment option for this devastating disease. In this study we characterize the anti-glioma effect of VCN-01, an improved hyaluronidase-armed pRB-pathway-selective oncolytic adenovirus that has proven safe and effective in the treatment of several solid tumors. VCN-01 displayed a significant cytotoxic effect on glioma cells in vitro. In vivo, in two different orthotopic glioma models, a single intra-tumoral administration of VCN-01 increased overall survival significantly and led to long-term survivors free of disease.

  18. A complex adenovirus vaccine against chikungunya virus provides complete protection against viraemia and arthritis

    Science.gov (United States)

    Wang, Danher; Suhrbier, Andreas; Penn-Nicholson, Adam; Woraratanadharm, Jan; Gardner, Joy; Luo, Min; Le, Thuy T.; Anraku, Itaru; Sakalian, Michael; Einfeld, David; Dong, John Y.

    2011-01-01

    Chikungunya virus, a mosquito-borne alphavirus, recently caused the largest epidemic ever seen for this virus. Chikungunya disease primarily manifests as a painful and debilitating arthralgia/arthritis, and no effective drug or vaccine is currently available. Here we describe a recombinant chikungunya virus vaccine comprising a non-replicating complex adenovirus vector encoding the structural polyprotein cassette of chikungunya virus. A single immunisation with this vaccine consistently induced high titres of anti-chikungunya virus antibodies that neutralised both an old Asian isolate and a Réunion Island isolate from the recent epidemic. The vaccine also completely protected mice against viraemia and arthritic disease caused by both virus isolates. PMID:21320541

  19. Radiation enhanced reactivation of irradiated human adenovirus type 2 in human cells

    International Nuclear Information System (INIS)

    Jeeves, W.P.

    1981-04-01

    Radiation-enhanced reactivation (ER) of a radiation-damaged mammalian virus is the term given to the observation that the survival of irradiated virus can be enhanced by irradiation of an appropriate host cell prior to infection. In this work, both UV-enhanced reactivation (UVER) and gamma-ray-enhanced reactivation (γRER) of irradiated human adenovirus type 2 (AD 2) were studied in a variety of normal and DNA repair-deficient human fibroblast host cell strains. In order to examine the lesion specificity of ER in human cells, experiments were performed using UV-irradiated and γ-irradiated virus. The investigation was carried out using a sensitive technique of indirect immunofluorescence, according to which irradiated and unirradiated cell cultures were infected with irradiated or unirradiated AD 2 and were subsequently examined for the presence of viral structural antigens ('V' Ag) at a fixed time after infection

  20. Estudio preliminar de los flebótomos de Villeta y Quebradanegra, Cundinamarca: sus implicaciones en salud pública

    Directory of Open Access Journals (Sweden)

    Raúl H. Pardo

    1996-12-01

    Full Text Available Con el fin de preseleccionar los posibles vectores de leishmaniasis cutánea en Villeta y su área rural más cercana, se hizo un reconocimiento de las especies del género Lutzomyia. Los insectos se capturaron durante la noche con cebo humano, cebo animal, trampas Shannon y trampas CDC y, durante el día, por aspiración directa en los sitios de reposo. Se encontraron ocho especies en los 497 insectos recolectados; las más abundantes fueron Lu. ovallesi (48,7%, Lu. gomezi (38,5% y Lu. lichyi (8%, especies reconocidas como antropofílicas y con antecedentes de ser vectoras o posibles vectoras. Lu. gomezifue la especie más abundante en las capturas con cebo humano, con una fuerte tendencia peridomiciliaria por lo que podría estar implicada en la transmisión de leishmaniasis en toda el área de estudio. Lu. ovallesi fue más abundante en los bosques poco intervenidos del área rural, por lo que es posible que juegue un papel como vector más importante en esta área. Lu. lichyise presentó, principalmente, en el bosque aunque llegó hasta el peridomicilio. Finalmente, es necesario iniciar estudios detallados para aclarar el papel que, como vectores, puedan tener las especies de Lutzomyia antes mencionadas en Villeta y el área rural adyacente.