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Sample records for coli iii papel

  1. Induction of genetic recombination in the lambda bacteriophage by ultraviolet irradiation of the Escherichia Coli cells. III. Role of the ruvA and recN genes; Induccion de recombinacion genetica en el bacteriofago lambda por irradiacion ultravioleta de las celulas de Escherichia Coli. III. Papel de los genes ruvA and recN

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    Alcantara D, D. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    1987-05-15

    The objective of this work is to determine the paper of the genes ruvA and recN in the stimulation of the recombination of Lambda for UV irradiation of Escherichia Coli, taking into account that both genes are inducible, they belong to the group of genes that participate in the SOS response and that a deficiency in its expression reduces the capacity to repair and recombiner the DNA. (Author)

  2. Deoxyribonucleic acid polymerase III of Escherichia coli. Purification and properties.

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    Livingston, D M; Hinkle, D C; Richardson, C C

    1975-01-25

    DNA polymerase III has been purified 4,500-fold from the Escherichis coli mutant, HMS83, which lacks DNA polymerases I and II. When subjected to disc gel electrophoresis, the most purified fraction exhibits a single major protein band from which enzymatic activity may be recovered. Polyacrylamide gel electrophoresis under denaturing conditions produces two protein bands with molecular weights of 140,000 and 40,000. The sedimentation coefficient of the enzyme is 7.0 S, and the Stokes radius is 62 A. Taken together these tow parameters indicate a native molecular weight of 180,000. Purified DNA polymerase III catalyzes the polymerization of nucleotides into DNA when provided with both a DNA template and a complementary primer strand. The newly synthesized DNA is covalently attached to the 3' terminus of the primer strand. Because the extent of polymerization is only 10 to 100 nucleotides, the best substrates are native DNA molecules with small single-stranded regions. The most purified enzyme preparation is devoid of endonuclease activities. In addition to the two exonuclease activities described in the accompanying paper, purified polymerase III also catalyzes pyrophosphorolysis and the exchange of pyrophosphate into deoxynucleoside triphosphates. DNA polymerase III has also been isolated from wild type E. coli containing the other two known DNA polymerases. Futhermore, the enzyme purified from three different polC mutants exhibits altered polymerase III activity, confirming that polC is the structural gene for DNA polymerase III (Gefter, M., Hirota, Y., Kornberb, T., Wechsler, J., and Barnoux, C. (1971) Proc. Natl. Acad. Sci. U. S. A. 68, 3150-3153).

  3. RNase III controls the degradation of corA mRNA in Escherichia coli.

    Science.gov (United States)

    Lim, Boram; Sim, Se-Hoon; Sim, Minji; Kim, Kyungsub; Jeon, Che Ok; Lee, Younghoon; Ha, Nam-Chul; Lee, Kangseok

    2012-05-01

    In Escherichia coli, the corA gene encodes a transporter that mediates the influx of Co(2+), Mg(2+), and Ni(2+) into the cell. During the course of experiments aimed at identifying RNase III-dependent genes in E. coli, we observed that steady-state levels of corA mRNA as well as the degree of cobalt influx into the cell were dependent on cellular concentrations of RNase III. In addition, changes in corA expression levels by different cellular concentrations of RNase III were closely correlated with degrees of resistance of E. coli cells to Co(2+) and Ni(2+). In vitro and in vivo cleavage analyses of corA mRNA identified RNase III cleavage sites in the 5'-untranslated region of the corA mRNA. The introduction of nucleotide substitutions at the identified RNase III cleavage sites abolished RNase III cleavage activity on corA mRNA and resulted in prolonged half-lives of the mRNA, which demonstrates that RNase III cleavage constitutes a rate-determining step for corA mRNA degradation. These findings reveal an RNase III-mediated regulatory pathway that functions to modulate corA expression and, in turn, the influx of metal ions transported by CorA in E. coli.

  4. RNase III Controls the Degradation of corA mRNA in Escherichia coli

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    Lim, Boram; Sim, Se-Hoon; Sim, Minji; Kim, Kyungsub; Jeon, Che Ok; Lee, Younghoon; Ha, Nam-Chul; Lee, Kangseok

    2012-01-01

    In Escherichia coli, the corA gene encodes a transporter that mediates the influx of Co2+, Mg2+, and Ni2+ into the cell. During the course of experiments aimed at identifying RNase III-dependent genes in E. coli, we observed that steady-state levels of corA mRNA as well as the degree of cobalt influx into the cell were dependent on cellular concentrations of RNase III. In addition, changes in corA expression levels by different cellular concentrations of RNase III were closely correlated with...

  5. RNase III-Independent Autogenous Regulation of Escherichia coli Polynucleotide Phosphorylase via Translational Repression

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    Carzaniga, T.; Dehò, G; Briani, F.

    2015-01-01

    The complex posttranscriptional regulation mechanism of the Escherichia coli pnp gene, which encodes the phosphorolytic exoribonuclease polynucleotide phosphorylase (PNPase), involves two endoribonucleases, namely, RNase III and RNase E, and PNPase itself, which thus autoregulates its own expression. The models proposed for pnp autoregulation posit that the target of PNPase is a mature pnp mRNA previously processed at its 5′ end by RNase III, rather than the primary pnp transcript (RNase I...

  6. RNase III-Independent Autogenous Regulation of Escherichia coli Polynucleotide Phosphorylase via Translational Repression.

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    Carzaniga, Thomas; Dehò, Gianni; Briani, Federica

    2015-06-01

    The complex posttranscriptional regulation mechanism of the Escherichia coli pnp gene, which encodes the phosphorolytic exoribonuclease polynucleotide phosphorylase (PNPase), involves two endoribonucleases, namely, RNase III and RNase E, and PNPase itself, which thus autoregulates its own expression. The models proposed for pnp autoregulation posit that the target of PNPase is a mature pnp mRNA previously processed at its 5' end by RNase III, rather than the primary pnp transcript (RNase III-dependent models), and that PNPase activity eventually leads to pnp mRNA degradation by RNase E. However, some published data suggest that pnp expression may also be regulated through a PNPase-dependent, RNase III-independent mechanism. To address this issue, we constructed isogenic Δpnp rnc(+) and Δpnp Δrnc strains with a chromosomal pnp-lacZ translational fusion and measured β-galactosidase activity in the absence and presence of PNPase expressed by a plasmid. Our results show that PNPase also regulates its own expression via a reversible RNase III-independent pathway acting upstream from the RNase III-dependent branch. This pathway requires the PNPase RNA binding domains KH and S1 but not its phosphorolytic activity. We suggest that the RNase III-independent autoregulation of PNPase occurs at the level of translational repression, possibly by competition for pnp primary transcript between PNPase and the ribosomal protein S1. In Escherichia coli, polynucleotide phosphorylase (PNPase, encoded by pnp) posttranscriptionally regulates its own expression. The two models proposed so far posit a two-step mechanism in which RNase III, by cutting the leader region of the pnp primary transcript, creates the substrate for PNPase regulatory activity, eventually leading to pnp mRNA degradation by RNase E. In this work, we provide evidence supporting an additional pathway for PNPase autogenous regulation in which PNPase acts as a translational repressor independently of RNase III

  7. A structural role for the PHP domain in E. coli DNA polymerase III.

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    Barros, Tiago; Guenther, Joel; Kelch, Brian; Anaya, Jordan; Prabhakar, Arjun; O'Donnell, Mike; Kuriyan, John; Lamers, Meindert H

    2013-05-14

    In addition to the core catalytic machinery, bacterial replicative DNA polymerases contain a Polymerase and Histidinol Phosphatase (PHP) domain whose function is not entirely understood. The PHP domains of some bacterial replicases are active metal-dependent nucleases that may play a role in proofreading. In E. coli DNA polymerase III, however, the PHP domain has lost several metal-coordinating residues and is likely to be catalytically inactive. Genomic searches show that the loss of metal-coordinating residues in polymerase PHP domains is likely to have coevolved with the presence of a separate proofreading exonuclease that works with the polymerase. Although the E. coli Pol III PHP domain has lost metal-coordinating residues, the structure of the domain has been conserved to a remarkable degree when compared to that of metal-binding PHP domains. This is demonstrated by our ability to restore metal binding with only three point mutations, as confirmed by the metal-bound crystal structure of this mutant determined at 2.9 Å resolution. We also show that Pol III, a large multi-domain protein, unfolds cooperatively and that mutations in the degenerate metal-binding site of the PHP domain decrease the overall stability of Pol III and reduce its activity. While the presence of a PHP domain in replicative bacterial polymerases is strictly conserved, its ability to coordinate metals and to perform proofreading exonuclease activity is not, suggesting additional non-enzymatic roles for the domain. Our results show that the PHP domain is a major structural element in Pol III and its integrity modulates both the stability and activity of the polymerase.

  8. Single‐molecule mechanochemical characterization of E. coli pol III core catalytic activity

    Science.gov (United States)

    Naufer, M. Nabuan; Murison, David A.; Rouzina, Ioulia; Beuning, Penny J.

    2017-01-01

    Abstract Pol III core is the three‐subunit subassembly of the E. coli replicative DNA polymerase III holoenzyme. It contains the catalytic polymerase subunit α, the 3′ → 5′ proofreading exonuclease ε, and a subunit of unknown function, θ. We employ optical tweezers to characterize pol III core activity on a single DNA substrate. We observe polymerization at applied template forces F  30 pN. Both polymerization and exonucleolysis occur as a series of short bursts separated by pauses. For polymerization, the initiation rate after pausing is independent of force. In contrast, the exonucleolysis initiation rate depends strongly on force. The measured force and concentration dependence of exonucleolysis initiation fits well to a two‐step reaction scheme in which pol III core binds bimolecularly to the primer‐template junction, then converts at rate k 2 into an exo‐competent conformation. Fits to the force dependence of k init show that exo initiation requires fluctuational opening of two base pairs, in agreement with temperature‐ and mismatch‐dependent bulk biochemical assays. Taken together, our results support a model in which the pol and exo activities of pol III core are effectively independent, and in which recognition of the 3′ end of the primer by either α or ε is governed by the primer stability. Thus, binding to an unstable primer is the primary mechanism for mismatch recognition during proofreading, rather than an alternative model of duplex defect recognition. PMID:28263430

  9. Pore-forming Activity of the Escherichia coli Type III Secretion System Protein EspD.

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    Chatterjee, Abhishek; Caballero-Franco, Celia; Bakker, Dannika; Totten, Stephanie; Jardim, Armando

    2015-10-16

    Enterohemorrhagic Escherichia coli is a causative agent of gastrointestinal and diarrheal diseases. Pathogenesis associated with enterohemorrhagic E. coli involves direct delivery of virulence factors from the bacteria into epithelial cell cytosol via a syringe-like organelle known as the type III secretion system. The type III secretion system protein EspD is a critical factor required for formation of a translocation pore on the host cell membrane. Here, we show that recombinant EspD spontaneously integrates into large unilamellar vesicle (LUV) lipid bilayers; however, pore formation required incorporation of anionic phospholipids such as phosphatidylserine and an acidic pH. Leakage assays performed with fluorescent dextrans confirmed that EspD formed a structure with an inner diameter of ∼2.5 nm. Protease mapping indicated that the two transmembrane helical hairpin of EspD penetrated the lipid layer positioning the N- and C-terminal domains on the extralumenal surface of LUVs. Finally, a combination of glutaraldehyde cross-linking and rate zonal centrifugation suggested that EspD in LUV membranes forms an ∼280-320-kDa oligomeric structure consisting of ∼6-7 subunits.

  10. Structural characterization of CFA/III and Longus type IVb pili from enterotoxigenic Escherichia coli.

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    Kolappan, Subramaniapillai; Roos, Justin; Yuen, Alex S W; Pierce, Owen M; Craig, Lisa

    2012-05-01

    The type IV pili are helical filaments found on many Gram-negative pathogenic bacteria, with multiple diverse roles in pathogenesis, including microcolony formation, adhesion, and twitching motility. Many pathogenic enterotoxigenic Escherichia coli (ETEC) isolates express one of two type IV pili belonging to the type IVb subclass: CFA/III or Longus. Here we show a direct correlation between CFA/III expression and ETEC aggregation, suggesting that these pili, like the Vibrio cholerae toxin-coregulated pili (TCP), mediate microcolony formation. We report a 1.26-Å resolution crystal structure of CofA, the major pilin subunit from CFA/III. CofA is very similar in structure to V. cholerae TcpA but possesses a 10-amino-acid insertion that replaces part of the α2-helix with an irregular loop containing a 3(10)-helix. Homology modeling suggests a very similar structure for the Longus LngA pilin. A model for the CFA/III pilus filament was generated using the TCP electron microscopy reconstruction as a template. The unique 3(10)-helix insert fits perfectly within the gap between CofA globular domains. This insert, together with differences in surface-exposed residues, produces a filament that is smoother and more negatively charged than TCP. To explore the specificity of the type IV pilus assembly apparatus, CofA was expressed heterologously in V. cholerae by replacing the tcpA gene with that of cofA within the tcp operon. Although CofA was synthesized and processed by V. cholerae, no CFA/III filaments were detected, suggesting that the components of the type IVb pilus assembly system are highly specific to their pilin substrates.

  11. Yeast redoxyendonuclease, a DNA repair enzyme similar to Escherichia coli endonuclease III

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    Gossett, J.; Lee, K.; Cunningham, R.P.; Doetsch, P.W.

    1988-04-05

    A DNA repair endonuclease (redoxyendonuclease) was isolated from bakers' yeast (Saccharomyces cerevisiae). The enzyme has been purified by a series of column chromatography steps and cleaves OsO/sub 4/-damaged, double-stranded DNA at sites of thymine glycol and heavily UV-irradiated DNA at sites of cytosine, thymine, and guanine photoproducts. The base specificity and mechanism of phosphodiester bond cleavage for the yeast redoxyendonuclease appear to be identical with those of Escherichia coli endonuclease III when thymine glycol containing, end-labeled DNA fragments of defined sequence are employed as substrates. Yeast redoxyendonuclease has an apparent molecular size of 38,000-42,000 daltons and is active in the absence of divalent metal cations. The identification of such an enzyme in yeast may be of value in the elucidation of the biochemical basis for radiation sensitivity in certain yeast mutants.

  12. Lysogeny with Shiga toxin 2-encoding bacteriophages represses type III secretion in enterohemorrhagic Escherichia coli.

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    Xu, Xuefang; McAteer, Sean P; Tree, Jai J; Shaw, Darren J; Wolfson, Eliza B K; Beatson, Scott A; Roe, Andrew J; Allison, Lesley J; Chase-Topping, Margo E; Mahajan, Arvind; Tozzoli, Rosangela; Woolhouse, Mark E J; Morabito, Stefano; Gally, David L

    2012-01-01

    Lytic or lysogenic infections by bacteriophages drive the evolution of enteric bacteria. Enterohemorrhagic Escherichia coli (EHEC) have recently emerged as a significant zoonotic infection of humans with the main serotypes carried by ruminants. Typical EHEC strains are defined by the expression of a type III secretion (T3S) system, the production of Shiga toxins (Stx) and association with specific clinical symptoms. The genes for Stx are present on lambdoid bacteriophages integrated into the E. coli genome. Phage type (PT) 21/28 is the most prevalent strain type linked with human EHEC infections in the United Kingdom and is more likely to be associated with cattle shedding high levels of the organism than PT32 strains. In this study we have demonstrated that the majority (90%) of PT 21/28 strains contain both Stx2 and Stx2c phages, irrespective of source. This is in contrast to PT 32 strains for which only a minority of strains contain both Stx2 and 2c phages (28%). PT21/28 strains had a lower median level of T3S compared to PT32 strains and so the relationship between Stx phage lysogeny and T3S was investigated. Deletion of Stx2 phages from EHEC strains increased the level of T3S whereas lysogeny decreased T3S. This regulation was confirmed in an E. coli K12 background transduced with a marked Stx2 phage followed by measurement of a T3S reporter controlled by induced levels of the LEE-encoded regulator (Ler). The presence of an integrated Stx2 phage was shown to repress Ler induction of LEE1 and this regulation involved the CII phage regulator. This repression could be relieved by ectopic expression of a cognate CI regulator. A model is proposed in which Stx2-encoding bacteriophages regulate T3S to co-ordinate epithelial cell colonisation that is promoted by Stx and secreted effector proteins.

  13. Lysogeny with Shiga toxin 2-encoding bacteriophages represses type III secretion in enterohemorrhagic Escherichia coli.

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    Xuefang Xu

    Full Text Available Lytic or lysogenic infections by bacteriophages drive the evolution of enteric bacteria. Enterohemorrhagic Escherichia coli (EHEC have recently emerged as a significant zoonotic infection of humans with the main serotypes carried by ruminants. Typical EHEC strains are defined by the expression of a type III secretion (T3S system, the production of Shiga toxins (Stx and association with specific clinical symptoms. The genes for Stx are present on lambdoid bacteriophages integrated into the E. coli genome. Phage type (PT 21/28 is the most prevalent strain type linked with human EHEC infections in the United Kingdom and is more likely to be associated with cattle shedding high levels of the organism than PT32 strains. In this study we have demonstrated that the majority (90% of PT 21/28 strains contain both Stx2 and Stx2c phages, irrespective of source. This is in contrast to PT 32 strains for which only a minority of strains contain both Stx2 and 2c phages (28%. PT21/28 strains had a lower median level of T3S compared to PT32 strains and so the relationship between Stx phage lysogeny and T3S was investigated. Deletion of Stx2 phages from EHEC strains increased the level of T3S whereas lysogeny decreased T3S. This regulation was confirmed in an E. coli K12 background transduced with a marked Stx2 phage followed by measurement of a T3S reporter controlled by induced levels of the LEE-encoded regulator (Ler. The presence of an integrated Stx2 phage was shown to repress Ler induction of LEE1 and this regulation involved the CII phage regulator. This repression could be relieved by ectopic expression of a cognate CI regulator. A model is proposed in which Stx2-encoding bacteriophages regulate T3S to co-ordinate epithelial cell colonisation that is promoted by Stx and secreted effector proteins.

  14. Escherichia coli Type III Secretion System 2 ATPase EivC Is Involved in the Motility and Virulence of Avian Pathogenic Escherichia coli

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    Wang, Shaohui; Liu, Xin; Xu, Xuan; Yang, Denghui; Wang, Dong; Han, Xiangan; Shi, Yonghong; Tian, Mingxing; Ding, Chan; Peng, Daxin; Yu, Shengqing

    2016-01-01

    Type III secretion systems (T3SSs) are crucial for bacterial infections because they deliver effector proteins into host cells. The Escherichia coli type III secretion system 2 (ETT2) is present in the majority of E. coli strains, and although it is degenerate, ETT2 regulates bacterial virulence. An ATPase is essential for T3SS secretion, but the function of the ETT2 ATPase has not been demonstrated. Here, we show that EivC is homologous to the β subunit of F0F1 ATPases and it possesses ATPase activity. To investigate the effects of ETT2 ATPase EivC on the phenotype and virulence of avian pathogenic Escherichia coli (APEC), eivC mutant and complemented strains were constructed and characterized. Inactivation of eivC led to impaired flagella production and augmented fimbriae on the bacterial surface, and, consequently, reduced bacterial motility. In addition, the eivC mutant strain exhibited attenuated virulence in ducks, diminished serum resistance, reduced survival in macrophage cells and in ducks, upregulated fimbrial gene expression, and downregulated flagellar and virulence gene expression. The expression of the inflammatory cytokines interleukin (IL)-1β and IL-8 were increased in HD-11 macrophages infected with the eivC mutant strain, compared with the wild-type strain. These virulence-related phenotypes were restored by genetic complementation. These findings demonstrate that ETT2 ATPase EivC is involved in the motility and pathogenicity of APEC. PMID:27630634

  15. Effects of ELF magnetic field in combination with Iron(III) chloride (FeCl3) on cellular growth and surface morphology of Escherichia coli (E. coli).

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    Esmekaya, Meric A; Acar, S Ipek; Kıran, Fadime; Canseven, Ayşe G; Osmanagaoglu, Ozlem; Seyhan, Nesrin

    2013-04-01

    This study investigated the effects of extremely low frequency (ELF) magnetic field with/without iron(III) chloride (FeCl3) on bacterial growth and morphology. The ELF exposures were carried out using a pair of Helmholtz coil-based ELF exposure system which was designed to generate 50 Hz sinusoidal magnetic field. The field was approximately uniform throughout the axis of the coil pair. The samples which were treated or non-treated with different concentrations FeCl3 were exposed to 50 Hz, 2 millitesla (mT) magnetic field for 24 h. ELF effect on viability was assessed in terms of viable colony counts (in colony-forming unit per milliliter) with the standard plate count technique. Scanning electron microscopy was used to investigate the magnetic field effect on surface morphology of Escherichia coli. No significant results were seen in terms of cell viability between ELF and sham-exposed bacterial strains. Similarly, FeCl3 treatment did not change cell viability of E. coli samples. However, we observed some morphological changes on E. coli cell surfaces. Pore formations and membrane destruction were seen on the surface of 24 h ELF field-exposed cells. We concluded that ELF magnetic field exposure at 2 mT does not affect cell viability; however, it may affect bacterial surface morphology.

  16. Exonuclease III and the catalase hydroperoxidase II in Escherichia coli are both regulated by the katF gene product

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    Sak, B.D.; Eisenstark, A.; Touati, D.

    1989-05-01

    The levels of both exonuclease III (exo III, product of xthA) and hydroperoxidase II (HP-II, product of katE) activity in Escherichia coli were influenced by a functional katF gene. The katF gene product is also necessary for synthesis of HP-II. Mutations in either katF or xthA, but not katE, result in sensitivity to H/sub 2/O/sub 2/ and near-UV (300-400 nm) radiation. Exo III, encoded by the xthA locus, recognizes and removes nucleoside 5'-monophosphates near apurinic and apyrimidinic sites in damaged DNA. Extracts of katF mutant strains had little detectable exo III activity. When a katF+ plasmid was introduced into the katF mutant, exo III activity exceeded wild-type levels. We propose that the katF gene is a trans-acting positive regulator of exo III and HP-II enzymes, both of which are involved in cellular recovery from oxidative damage.

  17. Production of uroporphyrinogen III, which is the common precursor of all tetrapyrrole cofactors, from 5-aminolevulinic acid by Escherichia coli expressing thermostable enzymes.

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    Hibino, Aiko; Petri, René; Büchs, Jochen; Ohtake, Hisao

    2013-08-01

    Uroporphyrinogen III (urogen III) was produced from 5-aminolevulinic acid (ALA), which is a common precursor of all metabolic tetrapyrroles, using thermostable ALA dehydratase (ALAD), porphobilinogen deaminase (PBGD), and urogen III synthase (UROS) of Thermus thermophilus HB8. The UROS-coding gene (hemD₂) of T. thermophilus HB8 was identified by examining the gene product for its ability to produce urogen III in a coupled reaction with ALAD and PBGD. The genes encoding ALAD, PBGD, and UROS were separately expressed in Escherichia coli BL21 (DE3). To inactivate indigenous mesophilic enzymes, the E. coli transformants were heated at 70 °C for 10 min. The bioconversion of ALA to urogen III was performed using a mixture of heat-treated E. coli transformants expressing ALAD, PBGD, and UROS at a cell ratio of 1:1:1. When the total cell concentration was 7.5 g/l, the mixture of heat-treated E. coli transformants could convert about 88 % 10 mM ALA to urogen III at 60 °C after 4 h. Since eight ALA molecules are required for the synthesis of one porphyrin molecule, approximately 1.1 mM (990 mg/l) urogen III was produced from 10 mM ALA. The present technology has great potential to supply urogen III for the biocatalytic production of vitamin B₁₂.

  18. The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli.

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    Burgers, P M; Kornberg, A; Sakakibara, Y

    1981-09-01

    An Escherichia coli mutant, dnaN59, stops DNA synthesis promptly upon a shift to a high temperature; the wild-type dnaN gene carried in a transducing phage encodes a polypeptide of about 41,000 daltons [Sakakibara, Y. & Mizukami, T. (1980) Mol. Gen. Genet. 178, 541-553; Yuasa, S. & Sakakibara, Y. (1980) Mol. Gen. Genet. 180, 267-273]. We now find that the product of dnaN gene is the beta subunit of DNA polymerase III holoenzyme, the principal DNA synthetic multipolypeptide complex in E. coli. The conclusion is based on the following observations: (i) Extracts from dnaN59 cells were defective in phage phi X174 and G4 DNA synthesis after the mutant cells had been exposed to the increased temperature. (ii) The enzymatic defect was overcome by addition of purified beta subunit but not by other subunits of DNA polymerase III holoenzyme or by other replication proteins required for phi X174 DNA synthesis. (iii) Partially purified beta subunit from the dnaN mutant, unlike that from the wild type, was inactive in reconstituting the holoenzyme when mixed with the other purified subunits. (iv) Increased dosage of the dnaN gene provided by a plasmid carrying the gene raised cellular levels of the beta subunit 5- to 6-fold.

  19. The role of multihaem cytochromes in the respiration of nitrite in Escherichia coli and Fe(III) in Shewanella oneidensis.

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    Clarke, Thomas A; Holley, Tracey; Hartshorne, Robert S; Fredrickson, Jim K; Zachara, John M; Shi, Liang; Richardson, David J

    2008-10-01

    The periplasmic nitrite reductase system from Escherichia coli and the extracellular Fe(III) reductase system from Shewanella oneidensis contain multihaem c-type cytochromes as electron carriers and terminal reductases. The position and orientation of the haem cofactors in multihaem cytochromes from different bacteria often show significant conservation despite different arrangements of the polypeptide chain. We propose that the decahaem cytochromes of the iron reductase system MtrA, MtrC and OmcA comprise pentahaem 'modules' similar to the electron donor protein, NrfB, from E. coli. To demonstrate this, we have isolated and characterized the N-terminal pentahaem module of MtrA by preparing a truncated form containing five covalently attached haems. UV-visible spectroscopy indicated that all five haems were low-spin, consistent with the presence of bis-His ligand co-ordination as found in full-length MtrA.

  20. Structure of putrescine aminotransferase from Escherichia coli provides insights into the substrate specificity among class III aminotransferases.

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    Hyung Jin Cha

    Full Text Available YgjG is a putrescine aminotransferase enzyme that transfers amino groups from compounds with terminal primary amines to compounds with an aldehyde group using pyridoxal-5'-phosphate (PLP as a cofactor. Previous biochemical data show that the enzyme prefers primary diamines, such as putrescine, over ornithine as a substrate. To better understand the enzyme's substrate specificity, crystal structures of YgjG from Escherichia coli were determined at 2.3 and 2.1 Å resolutions for the free and putrescine-bound enzymes, respectively. Sequence and structural analyses revealed that YgjG forms a dimer that adopts a class III PLP-dependent aminotransferase fold. A structural comparison between YgjG and other class III aminotransferases revealed that their structures are similar. However, YgjG has an additional N-terminal helical structure that partially contributes to a dimeric interaction with the other subunit via a helix-helix interaction. Interestingly, the YgjG substrate-binding site entrance size and charge distribution are smaller and more hydrophobic than other class III aminotransferases, which suggest that YgjG has a unique substrate binding site that could accommodate primary aliphatic diamine substrates, including putrescine. The YgjG crystal structures provide structural clues to putrescine aminotransferase substrate specificity and binding.

  1. Structure of putrescine aminotransferase from Escherichia coli provides insights into the substrate specificity among class III aminotransferases.

    Science.gov (United States)

    Cha, Hyung Jin; Jeong, Jae-Hee; Rojviriya, Catleya; Kim, Yeon-Gil

    2014-01-01

    YgjG is a putrescine aminotransferase enzyme that transfers amino groups from compounds with terminal primary amines to compounds with an aldehyde group using pyridoxal-5'-phosphate (PLP) as a cofactor. Previous biochemical data show that the enzyme prefers primary diamines, such as putrescine, over ornithine as a substrate. To better understand the enzyme's substrate specificity, crystal structures of YgjG from Escherichia coli were determined at 2.3 and 2.1 Å resolutions for the free and putrescine-bound enzymes, respectively. Sequence and structural analyses revealed that YgjG forms a dimer that adopts a class III PLP-dependent aminotransferase fold. A structural comparison between YgjG and other class III aminotransferases revealed that their structures are similar. However, YgjG has an additional N-terminal helical structure that partially contributes to a dimeric interaction with the other subunit via a helix-helix interaction. Interestingly, the YgjG substrate-binding site entrance size and charge distribution are smaller and more hydrophobic than other class III aminotransferases, which suggest that YgjG has a unique substrate binding site that could accommodate primary aliphatic diamine substrates, including putrescine. The YgjG crystal structures provide structural clues to putrescine aminotransferase substrate specificity and binding.

  2. Evidence for the presence of a type III secretion system in diffusely adhering Escherichia coli (DAEC).

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    Kyaw, C M; De Araujo, C R; Lima, M R; Gondim, E G S; Brígido, M M; Giugliano, L G

    2003-07-01

    Diffusely adhering Escherichia coli (E. coli) strains (DAEC) represent a potential cause of diarrhoea in infants, and the detection of type three secretion system (TTSS) genes in DAEC would substantiate their pathogenic nature. In this work, four isolates of DAEC, recovered from stools of diarrhoeic children, were analysed by PCR, in order to detect the presence of TTSS genes. Primers targeted to the escC, escJ, escN and escV, some of the most conserved TTSS genes in enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC), were used in order to verify the occurrence of homologous genes in our DAEC isolates. By this approach, we were able to characterise DNA fragments corresponding to putative escJ and escN genes in all DAEC isolates. Furthermore, DNA fragments homologous to the escC and escV genes were also amplified from all isolates. Besides the similarity found among the DAEC esc homologues with EPEC and EHEC esc genes, the nucleotide sequence analysis of the flanking regions of the amplified DNA fragments suggests that the putative DAEC esc genes are organised in the same manner as observed in EPEC and in EHEC strains. The results described here provide strong evidence for the presence of a TTSS in the DAEC strains analysed, implicating a pathogenic nature of these isolates.

  3. FACTORS LIMITING BACTERIAL GROWTH : III. CELL SIZE AND "PHYSIOLOGIC YOUTH" IN BACTERIUM COLI CULTURES.

    Science.gov (United States)

    Hershey, A D; Bronfenbrenner, J

    1938-07-20

    1. Measurements of the rate of oxygen uptake per cell in transplants of Bacterium coli from cultures of this organism in different phases of growth have given results in essential agreement with the observations of others. 2. Correlations of viable count, centrifugable nitrogen, and turbidity, with oxygen consumption, indicate that the increased metabolism during the early portion of the growth period is quantitatively referable to increased average size of cells. 3. Indirect evidence has suggested that the initial rate of growth of transplants is not related to the phase of growth of the parent culture.

  4. Iron(III) species formed during iron(II) oxidation and iron-core formation in bacterioferritin of Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Hawkins, C. [Sheffield Univ. (United Kingdom). Dep. of Physics; Treffry, A. [Sheffield Univ. (United Kingdom). Dep. of Molecular Biology and Biotechnology; Mackey, J.; Williams, J.M. [Sheffield Univ. (United Kingdom). Dep. of Physics; Andrews, S.C.; Guest, J.R.; Harrison, P.M. [Sheffield Univ. (United Kingdom). Dep. of Molecular Biology and Biotechnology

    1996-02-01

    This paper describes a preliminary investigation of the mechanisms of Fe(II) oxidation and storage of Fe(III) in the bacterioferritin of Escherichia coli (EcBFR). Using Moessbauer spectroscopy to examine the initial oxidation of iron by EcBFR it is confirmed that this ferritin exhibits ferroxidase activity and is shown that dimeric and monomeric iron species are produced as intermediates. The characteristics of ferroxidase activity in EcBFR is compare d with those of human H-chain ferritin (HuHF) and discuss the different Moessbauer parameters of their dimeric iron with reference to the structures of their di-metal sites. In addition, it is presented preliminary findings suggesting that after an initial {sup b}urst{sup ,} the rate of oxidation is greatly reduced, possibly due to blockage of the ferroxidase centre by bound iron. A new component, not found in HuHF and probably representing a small cluster of Fe(III) atoms, is reported.

  5. Two tandem RNase III cleavage sites determine betT mRNA stability in response to osmotic stress in Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Minji Sim

    Full Text Available While identifying genes regulated by ribonuclease III (RNase III in Escherichia coli, we observed that steady-state levels of betT mRNA, which encodes a transporter mediating the influx of choline, are dependent on cellular concentrations of RNase III. In the present study, we also observed that steady-state levels of betT mRNA are dependent on RNase III activity upon exposure to osmotic stress, indicating the presence of cis-acting elements controlled by RNase III in betT mRNA. Primer extension analyses of betT mRNA revealed two tandem RNase III cleavage sites in its stem-loop region, which were biochemically confirmed via in vitro cleavage assays. Analyses of cleavage sites suggested the stochastic selection of cleavage sites by RNase III, and mutational analyses indicated that RNase III cleavage at either site individually is insufficient for efficient betT mRNA degradation. In addition, both the half-life and abundance of betT mRNA were significantly increased in association with decreased RNase III activity under hyper-osmotic stress conditions. Our findings demonstrate that betT mRNA stability is controlled by RNase III at the post-transcriptional level under conditions of osmotic stress.

  6. Role of cortactin and the adaptor proteins NCK and CRK in pedestal formation by entherpathogenic "Escherichia coli" enteropatógena (EPEC). Papel de cortactina y de las proteínas adaptadoras Nck y Crk en la formación del pedestal por "Escherichia coli" enteropatógena (EPEC)

    OpenAIRE

    Nieto Pelegrín, Elvira

    2013-01-01

    La adherencia de la bacteria Escherichia coli enteropatógena (EPEC) a las células del epitelio intestinal conlleva la formación de estructuras ricas en filamentos de actina denominadas pedestales. El proceso requiere la translocación del efector bacteriano Tir (del inglés translocated intimin receptor) en el citoplasma de la célula hospedadora a través del sistema de secreción tipo III (TTSS). La inserción de Tir en la membrana plasmática de la célula conlleva la fosforilación en residuos de ...

  7. Distribution of CTX-M group I and group III β-lactamases produced by Escherichia coli and klebsiella pneumoniae in Lahore, Pakistan.

    Science.gov (United States)

    Abrar, Samyyia; Vajeeha, Ayesha; Ul-Ain, Noor; Riaz, Saba

    2017-02-01

    Extended-spectrum-lactamases (ESBLs) of the CTX-M type is worrisome issue in many countries of the world from past decade. But little is known about CTX-M beta-lactamase producing bacteria in Pakistan. Therefore, this study was carried out to investigate the distribution of CTX-M beta-lactamase producing E. coli and Klebsiella pneumoniae using phenotypic and molecular techniques. A total of 638 E. coli and 338 Klebsiella pneumoniae were isolated from patients attending two hospitals and one diagnostic Centre in Pakistan during 2013-2015. ESBL production was screened by double disc synergism, combination disc (cefotaxime and ceftazidime with clavulanic acid) and E-test. These strains were further characterized by PCR (CTX-M I, CTX-M III) and sequencing. After ribotyping of strains accession numbers were obtained. These isolates were highly resistant to cephalosporins, ceftazidime, cefotaxime, aztreonam, and cefuroxime but susceptible to carbapenems, sulfzone, amikacin and tazocin. Multiple antibiotic resistances index (MAR) revealed that 51% of E. coli strains fell in the range of 0.61-0.7 and 39% of Klebsiella pneumoniae strains fell in the range of 0.71-0.8. 64% Double disc synergism (DDS), 76.4% combination disc (CD), 74% E-test showed ESBL positivity in strains. In E. coli ESBL genes blaCTX-M-I and blaCTX-M-III were detected in 212 (72.1%) and 25 (8.5%) respectively. In Klebsiella pneumoniae ESBL genes blaCTX-M-I and blaCTX-M-III were detected in 89 (82.4%) and 10 (9.2%). Combination of both genes blaCTX-M-I and blaCTX-M-III were found in 16 (5.4%) of E. coli strains and 5 (4.6%) of Klebsiella pneumoniae strains. Sequencing revealed that CTXM-15 was predominately present in the CTX-M-I group. The prevalence of ESBL producing E. coli and Klebsiella pneumoniae isolates was high and the majority of them positive for blaCTX-M-I as compared to blaCTX-M-III. These findings highlight the need to further investigate the epidemiology of other CTX-M beta

  8. Distribution, functional expression, and genetic organization of Cif, a phage-encoded type III-secreted effector from enteropathogenic and enterohemorrhagic Escherichia coli.

    Science.gov (United States)

    Loukiadis, Estelle; Nobe, Rika; Herold, Sylvia; Tramuta, Clara; Ogura, Yoshitoshi; Ooka, Tadasuke; Morabito, Stefano; Kérourédan, Monique; Brugère, Hubert; Schmidt, Herbert; Hayashi, Tetsuya; Oswald, Eric

    2008-01-01

    Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) inject effector proteins into host cells via a type III secretion system encoded by the locus of enterocyte effacement (LEE). One of these effectors is Cif, encoded outside the LEE by a lambdoid prophage. In this study, we demonstrated that the Cif-encoding prophage of EPEC strain E22 is inducible and produces infectious phage particles. We investigated the distribution and functional expression of Cif in 5,049 E. coli strains of human, animal, and environmental origins. A total of 115 E. coli isolates from diverse origins and geographic locations carried cif. The presence of cif was tightly associated with the LEE, since all the cif-positive isolates were positive for the LEE. These results suggested that the Cif-encoding prophages have been widely disseminated within the natural population of E. coli but positively selected within the population of LEE-positive strains. Nonetheless, 66% of cif-positive E. coli strains did not induce a typical Cif-related phenotype in eukaryotic cells due to frameshift mutations or insertion of an IS element in the cif gene. The passenger region of the prophages carrying cif was highly variable and showed various combinations of IS elements and genes coding for other effectors such as nleB, nleC, nleH, nleG, espJ, and nleA/espI (some of which were also truncated). This diversity and the presence of nonfunctional effectors should be taken into account to assess EPEC and EHEC pathogenicity and tropism.

  9. Base excision of oxidative purine and pyrimidine DNA damage in Saccharomyces cerevisiae by a DNA glycosylase with sequence similarity to endonuclease III from Escherichia coli.

    Science.gov (United States)

    Eide, L; Bjørås, M; Pirovano, M; Alseth, I; Berdal, K G; Seeberg, E

    1996-10-01

    One gene locus on chromosome I in Saccharomyces cerevisiae encodes a protein (YAB5_YEAST; accession no. P31378) with local sequence similarity to the DNA repair glycosylase endonuclease III from Escherichia coli. We have analyzed the function of this gene, now assigned NTG1 (endonuclease three-like glycosylase 1), by cloning, mutant analysis, and gene expression in E. coli. Targeted gene disruption of NTG1 produces a mutant that is sensitive to H2O2 and menadione, indicating that NTG1 is required for repair of oxidative DNA damage in vivo. Northern blot analysis and expression studies of a NTG1-lacZ gene fusion showed that NTG1 is induced by cell exposure to different DNA damaging agents, particularly menadione, and hence belongs to the DNA damage-inducible regulon in S. cerevisiae. When expressed in E. coli, the NTG1 gene product cleaves plasmid DNA damaged by osmium tetroxide, thus, indicating specificity for thymine glycols in DNA similarly as is the case for EndoIII. However, NTG1 also releases formamidopyrimidines from DNA with high efficiency and, hence, represents a glycosylase with a novel range of substrate recognition. Sequences similar to NTG1 from other eukaryotes, including Caenorhabditis elegans, Schizosaccharomyces pombe, and mammals, have recently been entered in the GenBank suggesting the universal presence of NTG1-like genes in higher organisms. S. cerevisiae NTG1 does not have the [4Fe-4S] cluster DNA binding domain characteristic of the other members of this family.

  10. Enterohemorrhagic E. coli requires N-WASP for efficient type III translocation but not for EspFU-mediated actin pedestal formation.

    Directory of Open Access Journals (Sweden)

    Didier Vingadassalom

    Full Text Available Upon infection of mammalian cells, enterohemorrhagic E. coli (EHEC O157:H7 utilizes a type III secretion system to translocate the effectors Tir and EspF(U (aka TccP that trigger the formation of F-actin-rich 'pedestals' beneath bound bacteria. EspF(U is localized to the plasma membrane by Tir and binds the nucleation-promoting factor N-WASP, which in turn activates the Arp2/3 actin assembly complex. Although N-WASP has been shown to be required for EHEC pedestal formation, the precise steps in the process that it influences have not been determined. We found that N-WASP and actin assembly promote EHEC-mediated translocation of Tir and EspF(U into mammalian host cells. When we utilized the related pathogen enteropathogenic E. coli to enhance type III translocation of EHEC Tir and EspF(U, we found surprisingly that actin pedestals were generated on N-WASP-deficient cells. Similar to pedestal formation on wild type cells, Tir and EspF(U were the only bacterial effectors required for pedestal formation, and the EspF(U sequences required to interact with N-WASP were found to also be essential to stimulate this alternate actin assembly pathway. In the absence of N-WASP, the Arp2/3 complex was both recruited to sites of bacterial attachment and required for actin assembly. Our results indicate that actin assembly facilitates type III translocation, and reveal that EspF(U, presumably by recruiting an alternate host factor that can signal to the Arp2/3 complex, exhibits remarkable versatility in its strategies for stimulating actin polymerization.

  11. Cloning, expression, purification, crystallization and X-ray crystallographic analysis of CofB, the minor pilin subunit of CFA/III from human enterotoxigenic Escherichia coli.

    Science.gov (United States)

    Kawahara, Kazuki; Oki, Hiroya; Fukakusa, Shunsuke; Maruno, Takahiro; Kobayashi, Yuji; Motooka, Daisuke; Taniguchi, Tooru; Honda, Takeshi; Iida, Tetsuya; Nakamura, Shota; Ohkubo, Tadayasu

    2015-06-01

    Colonization factor antigen III (CFA/III) is one of the virulence factors of human enterotoxigenic Escherichia coli (ETEC) that forms the long, thin, proteinaceous fibres of type IV pili through assembly of its major and minor subunits CofA and CofB, respectively. The crystal structure of CofA has recently been reported; however, the lack of structural information for CofB, the largest among the known type IV pilin subunits, hampers a comprehensive understanding of CFA/III pili. In this study, constructs of wild-type CofB with an N-terminal truncation and the corresponding SeMet derivative were cloned, expressed, purified and crystallized. The crystals belonged to the rhombohedral space group R32, with unit-cell parameters a = b = 103.97, c = 364.57 Å for the wild-type construct and a = b = 103.47, c = 362.08 Å for the SeMet-derivatized form. Although the diffraction quality of these crystals was initially very poor, dehydration of the crystals substantially improved the resolution limit from ∼ 4.0 to ∼ 2.0 Å. The initial phase was solved by the single-wavelength anomalous dispersion (SAD) method using a dehydrated SeMet CofB crystal, which resulted in an interpretable electron-density map.

  12. Display of E. coli Alkaline Phosphatase pIII or pVIII Fusions on Phagemid Surfaces Reveals Monovalent Decoration with Active Molecules

    Science.gov (United States)

    Weichel, Michael; Jaussi, Rolf; Rhyner, Claudio; Crameri, Reto

    2008-01-01

    Active alkaline phosphatase of Escherichia coli (PhoA, EC 3.1.3.1) was displayed via the leucine zipper element of the Jun-Fos heterodimer on the surface of filamentous phage and the kinetic parameters Km and kcat were determined. The phoA gene was cloned downstream of fos while jun was inserted upstream of pIII or pVIII, alternatively, in the pJuFo phagemid vector. Both fusion genes are regulated by independent lacZ promoters. PhoA displayed on the phagemid pIII surface exhibited a Km of 11.2 µM with 4-nitrophenyl phosphate as substrate, which is consistent with data published for soluble PhoA. Based on these data we calculated the decoration of pJuFo phagemid with PhoA using the minor and major coat proteins pIII and pVIII as fusion partners under variable inducing conditions. We found that, even if the promoters are fully induced at a concentration of 1000 µM IPTG, the phagemids display maximally one copy of PhoA-Fos-Jun-coat protein fusion, irrespective of whether the protein is presented via pIII or pVIII. However, since PhoA is displayed in a native-like fashion, as deduced from the kinetic parameters of the enzymatic reaction, the pJuFo technology provides a versatile tool for the functional screening of complex cDNA libraries displayed on the phagemids' surface. PMID:18949073

  13. From ingestion to colonization: the influence of the host environment on regulation of the LEE encoded type III secretion system in enterohaemorrhagic Escherichia coli

    Directory of Open Access Journals (Sweden)

    James P R Connolly

    2015-06-01

    Full Text Available Enterohaemorrhagic Escherichia coli (EHEC binds to host tissue and intimately attaches to intestinal cells using a dedicated type III secretion system (T3SS. This complex multi-protein organelle is encoded within a large pathogenicity island called the locus of enterocyte effacement (LEE, which is subject to extensive regulatory control. Over the past 15 years we have gained a wealth of knowledge concerning how the LEE is regulated transcriptionally by specific, global and phage encoded regulators, but more recently, significant advances have been made in our understanding of how specific signals, including host or microbiota derived metabolic products and various nutrient sources, can affect how the LEE encoded T3SS is regulated. In this review we discuss transcriptional regulation of the LEE in EHEC, focusing on how these physiologically relevant signals are sensed and how they affect the expression of this major virulence factor. The implications for understanding the disease process by specific regulatory mechanisms are also discussed.

  14. From ingestion to colonization: the influence of the host environment on regulation of the LEE encoded type III secretion system in enterohaemorrhagic Escherichia coli.

    Science.gov (United States)

    Connolly, James P R; Finlay, B Brett; Roe, Andrew J

    2015-01-01

    Enterohaemorrhagic Escherichia coli (EHEC) binds to host tissue and intimately attaches to intestinal cells using a dedicated type III secretion system (T3SS). This complex multi-protein organelle is encoded within a large pathogenicity island called the locus of enterocyte effacement (LEE), which is subject to extensive regulatory control. Over the past 15 years we have gained a wealth of knowledge concerning how the LEE is regulated transcriptionally by specific, global and phage encoded regulators. More recently, significant advances have been made in our understanding of how specific signals, including host or microbiota derived metabolic products and various nutrient sources, can affect how the LEE-encoded T3SS is regulated. In this review we discuss regulation of the LEE, focusing on how these physiologically relevant signals are sensed and how they affect the expression of this major virulence factor. The implications for understanding the disease process by specific regulatory mechanisms are also discussed.

  15. Role of EscU auto-cleavage in promoting type III effector translocation into host cells by enteropathogenic Escherichia coli

    Science.gov (United States)

    2011-01-01

    Background Type III secretion systems (T3SS) of bacterial pathogens coordinate effector protein injection into eukaryotic cells. The YscU/FlhB group of proteins comprises members associated with T3SS which undergo a specific auto-cleavage event at a conserved NPTH amino acid sequence. The crystal structure of the C-terminal portion of EscU from enteropathogenic Escherichia coli (EPEC) suggests this auto-cleaving protein provides an interface for substrate interactions involved in type III secretion events. Results We demonstrate EscU must be auto-cleaved for bacteria to efficiently deliver type III effectors into infected cells. A non-cleaving EscU(N262A) variant supported very low levels of in vitro effector secretion. These effector proteins were not able to support EPEC infection of cultured HeLa cells. In contrast, EscU(P263A) was demonstrated to be partially auto-cleaved and moderately restored effector translocation and functionality during EPEC infection, revealing an intermediate phenotype. EscU auto-cleavage was not required for inner membrane association of the T3SS ATPase EscN or the ring forming protein EscJ. In contrast, in the absence of EscU auto-cleavage, inner membrane association of the multicargo type III secretion chaperone CesT was altered suggesting that EscU auto-cleavage supports docking of chaperone-effector complexes at the inner membrane. In support of this interpretation, evidence of novel effector protein breakdown products in secretion assays were linked to the non-cleaved status of EscU(N262A). Conclusions These data provide new insight into the role of EscU auto-cleavage in EPEC. The experimental data suggests that EscU auto-cleavage results in a suitable binding interface at the inner membrane that accommodates protein complexes during type III secretion events. The results also demonstrate that altered EPEC genetic backgrounds that display intermediate levels of effector secretion and translocation can be isolated and studied

  16. The bacteriophage P1 hot gene, encoding a homolog of the E. coli DNA polymerase III theta subunit, is expressed during both lysogenic and lytic growth stages.

    Science.gov (United States)

    Chikova, Anna K; Schaaper, Roel M

    2007-11-01

    The bacteriophage P1 hot gene product is a homolog of the theta subunit of E. coli DNA polymerase III. Previous studies with hot cloned on a plasmid have shown that Hot protein can substitute for theta, as evidenced by its stabilizing effect on certain dnaQ mutator mutants carrying an unstable pol III proofreading subunit (epsilon subunit). These results are consistent with Hot, like theta, being a replication protein involved in stabilizing the intrinsically unstable epsilon proofreading function. However, the function of hot for the viral life cycle is less clear. In the present study, we show that the hot gene is not essential. Based on its promoter structure, hot has been previously classified as a "late" phage gene, a property that is not easily reconciled with a presumed replication function. Here, we clarify this issue by demonstrating that P1 hot is actively expressed both during the lysogenic state and in the early stages of a lytic induction, in addition to its expression in the late stage of phage development. The results indicate that P1 hot has a complex expression pattern, compatible with a model in which Hot may affect the host replication machinery to benefit overall phage replication.

  17. O papel do enfermeiro hoje

    Directory of Open Access Journals (Sweden)

    Luzia Cecília de Medeiros

    1997-06-01

    Full Text Available Este trabalho tem por objetivo analisar o papel do enfermeiro como resultado das transformações históricas ocorridas na enfermagem. O sistema capitalista coloca ao enfermeiro, no modelo clínico de assistência, a função de administrar os serviços e a equipe de enfermagem restringindo assim sua função a primar pela lucratividade e burocracia da empresa. Dessa forma a assistência é delegada a outras categorias profissionais, muitas vezes sem nenhuma qualificação. Nesse universo, entendemos que o enfermeiro tem como papel principal administrar a assistência de enfermagem para ter uma visão do todo e dessa forma desenvolver uma prestação de serviços qualificada e articulada as atuais necessidades da estrutura social.

  18. Crystal Structure of the Minor Pilin CofB, the Initiator of CFA/III Pilus Assembly in Enterotoxigenic Escherichia coli.

    Science.gov (United States)

    Kolappan, Subramania; Ng, Dixon; Yang, Guixiang; Harn, Tony; Craig, Lisa

    2015-10-23

    Type IV pili are extracellular polymers of the major pilin subunit. These subunits are held together in the pilus filament by hydrophobic interactions among their N-terminal α-helices, which also anchor the pilin subunits in the inner membrane prior to pilus assembly. Type IV pilus assembly involves a conserved group of proteins that span the envelope of Gram-negative bacteria. Among these is a set of minor pilins, so named because they share their hydrophobic N-terminal polymerization/membrane anchor segment with the major pilins but are much less abundant. Minor pilins influence pilus assembly and retraction, but their precise functions are not well defined. The Type IV pilus systems of enterotoxigenic Escherichia coli and Vibrio cholerae are among the simplest of Type IV pilus systems and possess only a single minor pilin. Here we show that the enterotoxigenic E. coli minor pilins CofB and LngB are required for assembly of their respective Type IV pili, CFA/III and Longus. Low levels of the minor pilins are optimal for pilus assembly, and CofB can be detected in the pilus fraction. We solved the 2.0 Å crystal structure of N-terminally truncated CofB, revealing a pilin-like protein with an extended C-terminal region composed of two discrete domains connected by flexible linkers. The C-terminal region is required for CofB to initiate pilus assembly. We propose a model for CofB-initiated pilus assembly with implications for understanding filament growth in more complex Type IV pilus systems as well as the related Type II secretion system. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  19. The Serine Protease EspC from Enteropathogenic Escherichia coli Regulates Pore Formation and Cytotoxicity Mediated by the Type III Secretion System.

    Directory of Open Access Journals (Sweden)

    Julie Guignot

    2015-07-01

    Full Text Available Type III secretion systems (T3SSs are specialized macromolecular machines critical for bacterial virulence, and allowing the injection of bacterial effectors into host cells. The T3SS-dependent injection process requires the prior insertion of a protein complex, the translocon, into host cell membranes consisting of two-T3SS hydrophobic proteins, associated with pore-forming activity. In all described T3SS to date, a hydrophilic protein connects one hydrophobic component to the T3SS needle, presumably insuring the continuum between the hollow needle and the translocon. In the case of Enteropathogenic Escherichia coli (EPEC, the hydrophilic component EspA polymerizes into a filament connecting the T3SS needle to the translocon composed of the EspB and EspD hydrophobic proteins. Here, we identify EspA and EspD as targets of EspC, a serine protease autotransporter of Enterobacteriaceae (SPATE. We found that in vitro, EspC preferentially targets EspA associated with EspD, but was less efficient at proteolyzing EspA alone. Consistently, we found that EspC did not regulate EspA filaments at the surface of primed bacteria that was devoid of EspD, but controlled the levels of EspD and EspA secreted in vitro or upon cell contact. While still proficient for T3SS-mediated injection of bacterial effectors and cytoskeletal reorganization, an espC mutant showed increased levels of cell-associated EspA and EspD, as well as increased pore formation activity associated with cytotoxicity. EspP from enterohaemorrhagic E. coli (EHEC also targeted translocator components and its activity was interchangeable with that of EspC, suggesting a common and important function of these SPATEs. These findings reveal a novel regulatory mechanism of T3SS-mediated pore formation and cytotoxicity control during EPEC/EHEC infection.

  20. Homo-trimeric Structure of the Type IVb Minor Pilin CofB Suggests Mechanism of CFA/III Pilus Assembly in Human Enterotoxigenic Escherichia coli.

    Science.gov (United States)

    Kawahara, Kazuki; Oki, Hiroya; Fukakusa, Shunsuke; Yoshida, Takuya; Imai, Tomoya; Maruno, Takahiro; Kobayashi, Yuji; Motooka, Daisuke; Iida, Tetsuya; Ohkubo, Tadayasu; Nakamura, Shota

    2016-03-27

    In gram-negative bacteria, the assembly of type IV pilus (T4P) and the evolutionally related pseudopilus of type II secretion system involves specialized structural proteins called pilins and pseudopilins, respectively, and is dynamically regulated to promote bacterial pathogenesis. Previous studies have suggested that a structural "tip"-like hetero-complex formed through the interaction of at least three minor (pseudo) pilins plays an important role in this process, while some members of the pathogenic type IVb subfamily are known to have only one such minor pilin subunit whose function is still unknown. Here, we determined the crystal structure of the type IVb minor pilin CofB of colonization factor antigen/III from human enterotoxigenic Escherichia coli at 1.88-Å resolution. The crystal structure, in conjunction with physicochemical analysis in solution, reveals a symmetrical homo-trimeric arrangement distinct from the hetero-complexes of minor (pseudo) pilins observed in other T4P and type II secretion systems. Each CofB monomer adopts a unique three-domain architecture, in which the C-terminal β-sheet-rich lectin domain can effectively initiate trimer association of its pilin-like N-terminal domain through extensive hydrophobic interactions followed by domain swapping at the central hinge-like domain. Deletion of cofB produces a phenotype with no detectable pili formation on the cell surface, while molecular modeling indicates that the characteristic homo-trimeric structure of CofB is well situated at the pilus tip of colonization factor antigen/III formed by the major pilin CofA, suggesting a role for the minor pilin in the efficient initiation of T4P assembly. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. RNase III is required for localization to the nucleoid of the 5' pre-rRNA leader and for optimal induction of rRNA synthesis in E. coli.

    Science.gov (United States)

    Malagon, Francisco

    2013-09-01

    It has recently been demonstrated that ribosomes are preferentially localized outside the nucleoid in Escherichia coli, but little is known about the spatial regulation of pre-rRNA processing. In this work, I investigate the cellular distribution of leader pre-rRNAs using RNA-FISH. In contrast to mature rRNA, the 5' proximal leader region associates with the nucleoid, and this association occurs in an RNase III-dependent manner. Moreover, RNase III plays a role in the rapid induction of ribosomal operons during outgrowth and is essential in the absence of the transcriptional regulator Fis, suggesting a linkage of transcription and RNA processing for ribosomal operons in E. coli.

  2. RNase III is required for localization to the nucleoid of the 5′ pre-rRNA leader and for optimal induction of rRNA synthesis in E. coli

    Science.gov (United States)

    Malagon, Francisco

    2013-01-01

    It has recently been demonstrated that ribosomes are preferentially localized outside the nucleoid in Escherichia coli, but little is known about the spatial regulation of pre-rRNA processing. In this work, I investigate the cellular distribution of leader pre-rRNAs using RNA-FISH. In contrast to mature rRNA, the 5′ proximal leader region associates with the nucleoid, and this association occurs in an RNase III-dependent manner. Moreover, RNase III plays a role in the rapid induction of ribosomal operons during outgrowth and is essential in the absence of the transcriptional regulator Fis, suggesting a linkage of transcription and RNA processing for ribosomal operons in E. coli. PMID:23893733

  3. A two-dose regimen of a vaccine against Escherichia coli O157:H7 type III secreted proteins reduced environmental transmission of the agent in a large-scale commercial beef feedlot clinical trial.

    Science.gov (United States)

    Smith, David R; Moxley, Rodney A; Peterson, Robert E; Klopfenstein, Terry J; Erickson, Galen E; Clowser, Sharon L

    2008-10-01

    A clinical vaccine trial of commercially fed cattle tested the effect of a two-dose regimen of a vaccine product against type III secreted proteins of enterohemorrhagic Escherichia coli O157:H7 on the probability of detecting the organism on environmental sampling devices. Within commercial feedlots, pens of vaccinated and unvaccinated cattle were matched by reprocessing schedule and time of sampling. Vaccine was administered to all cattle within treated pens at arrival processing and again at re-implant processing. Pens of cattle were sampled 1 week after the second dose of vaccine and every 3 weeks for four test periods. Pair-matched pens of cattle were sampled concurrently. Test samples were seven ropes per pen hung overnight from the feed-bunk neck-rail (ROPES). Recovery of E. coli O157:H7 from at least one rope classified pens ROPES-positive. E. coli O157:H7 isolates were identified by standard biochemical methods and multiplex polymerase chain reaction. The probability for pens of cattle to test ROPES-positive was modeled using multilevel logistic regression with variance adjustment for clustering by matched pens and repeated measures. We studied 140 pens of cattle representing 20,556 cattle in 19 feedlots February through October 2004. Vaccinated pens of cattle were less likely to test ROPES-positive (OR = 0.59, p = 0.004). Because ROPES testing identifies organisms in the mouth of cattle, and the outcome is both associated with presence of the organism in the pen environment and correlated with the prevalence of fecal shedding, we conclude the two-dose vaccine regimen reduces the probability for environmental transmission of E. coli O157:H7 within commercial cattle feeding systems.

  4. Structure of the Cytoplasmic Loop between Putative Helices II and III of the Mannitol Permease of Escherichia coli : A Tryptophan and 5-Fluorotryptophan Spectroscopy Study

    NARCIS (Netherlands)

    Vos, Erwin P. P.; Bokhove, Marcel; Hesp, Ben H.; Broos, Jaap

    2009-01-01

    In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EII(mtl), are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be invol

  5. Potenciar el papel de los logaritmos

    OpenAIRE

    Fernández, Jorge

    1992-01-01

    Lo que pretendemos es destacar cómo se pueden integrar las calculadoras en el tema de los logaritmos, dando a éstas un papel como instrumento importante en su enseñanza y no como el elemento que ha llevado hacia su decadencia.

  6. In vitro colonization of the muscle extracellular matrix components by Escherichia coli O157:H7: the influence of growth medium, temperature and pH on initial adhesion and induction of biofilm formation by collagens I and III.

    Directory of Open Access Journals (Sweden)

    Caroline Chagnot

    Full Text Available Enterohemorrhagic Escherichia coli (EHEC O157:H7 are responsible for repeated food-poisoning cases often caused by contaminated burgers. EHEC infection is predominantly a pediatric illness, which can lead to life-threatening diseases. Ruminants are the main natural reservoir for EHEC and food contamination almost always originates from faecal contamination. In beef meat products, primary bacterial contamination occurs at the dehiding stage of slaughtering. The extracellular matrix (ECM is the most exposed part of the skeletal muscles in beef carcasses. Investigating the adhesion to the main muscle fibrous ECM proteins, insoluble fibronectin, collagen I, III and IV, laminin-α2 and elastin, results demonstrated that the preceding growth conditions had a great influence on subsequent bacterial attachment. In the tested experimental conditions, maximal adhesion to fibril-forming collagens I or III occurred at 25°C and pH 7. Once initially adhered, exposure to lower temperatures, as applied to meat during cutting and storage, or acidification, as in the course of post-mortem physiological modifications of muscle, had no effect on detachment, except at pHu. In addition, dense biofilm formation occurred on immobilized collagen I or III and was induced in growth medium supplemented with collagen I in solution. From this first comprehensive investigation of EHEC adhesion to ECM proteins with respect to muscle biology and meat processing, new research directions for the development of innovative practices to minimize the risk of meat contamination are further discussed.

  7. Solution study of the Escherichia coli DNA polymerase III clamp loader reveals the location of the dynamic ψχ heterodimer

    Directory of Open Access Journals (Sweden)

    Farzaneh Tondnevis

    2015-09-01

    Full Text Available Several X-ray crystal structures of the E. coli core clamp loader containing the five core (δ′, δ, and three truncated γ subunits have been determined, but they lack the ψ and χ subunits. We report the first solution structure of the complete seven-subunit clamp loader complex using small angle X-ray scattering. This structure not only provides information about the location of the χ and ψ subunits but also provides a model of the dynamic nature of the clamp loader complex.

  8. Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, ε, θ and β reveals a highly flexible arrangement of the proofreading domain

    Science.gov (United States)

    Ozawa, Kiyoshi; Horan, Nicholas P.; Robinson, Andrew; Yagi, Hiromasa; Hill, Flynn R.; Jergic, Slobodan; Xu, Zhi-Qiang; Loscha, Karin V.; Li, Nan; Tehei, Moeava; Oakley, Aaron J.; Otting, Gottfried; Huber, Thomas; Dixon, Nicholas E.

    2013-01-01

    A complex of the three (αεθ) core subunits and the β2 sliding clamp is responsible for DNA synthesis by Pol III, the Escherichia coli chromosomal DNA replicase. The 1.7 Å crystal structure of a complex between the PHP domain of α (polymerase) and the C-terminal segment of ε (proofreading exonuclease) subunits shows that ε is attached to α at a site far from the polymerase active site. Both α and ε contain clamp-binding motifs (CBMs) that interact simultaneously with β2 in the polymerization mode of DNA replication by Pol III. Strengthening of both CBMs enables isolation of stable αεθ:β2 complexes. Nuclear magnetic resonance experiments with reconstituted αεθ:β2 demonstrate retention of high mobility of a segment of 22 residues in the linker that connects the exonuclease domain of ε with its α-binding segment. In spite of this, small-angle X-ray scattering data show that the isolated complex with strengthened CBMs has a compact, but still flexible, structure. Photo-crosslinking with p-benzoyl-L-phenylalanine incorporated at different sites in the α-PHP domain confirm the conformational variability of the tether. Structural models of the αεθ:β2 replicase complex with primer-template DNA combine all available structural data. PMID:23580545

  9. An alternative reaction for heme degradation catalyzed by the Escherichia coli O157:H7 ChuS protein: Release of hematinic acid, tripyrrole and Fe(III).

    Science.gov (United States)

    Ouellet, Yannick H; Ndiaye, Cheikh Tidiane; Gagné, Stéphane M; Sebilo, Anne; Suits, Michael D L; Jubinville, Éric; Jia, Zongchao; Ivancich, Anabella; Couture, Manon

    2016-01-01

    As part of the machinery to acquire, internalize and utilize heme as a source of iron from the host, some bacteria possess a canonical heme oxygenase, where heme plays the dual role of substrate and cofactor, the later catalyzing the cleavage of the heme moiety using O2 and electrons, and resulting in biliverdin, carbon monoxide and ferrous non-heme iron. We have previously reported that the Escherichia coli O157:H7 ChuS protein, which is not homologous to heme oxygenases, can bind and degrade heme in a reaction that releases carbon monoxide. Here, we have pursued a detailed characterization of such heme degradation reaction using stopped-flow UV-visible absorption spectrometry, the characterization of the intermediate species formed in such reaction by EPR spectroscopy and the identification of reaction products by NMR spectroscopy and Mass spectrometry. We show that hydrogen peroxide (in molar equivalent) is the key player in the degradation reaction, at variance to canonical heme oxygenases. While the initial intermediates of the reaction of ChuS with hydrogen peroxide (a ferrous keto π neutral radical and ferric verdoheme, both identified by EPR spectroscopy) are in common with heme oxygenases, a further and unprecedented reaction step, involving the cleavage of the porphyrin ring at adjacent meso-carbons, results in the release of hematinic acid (a monopyrrole moiety identified by NMR spectroscopy), a tripyrrole product (identified by Mass spectrometry) and non-heme iron in the ferric oxidation state (identified by EPR spectroscopy). Overall, the unprecedented reaction of E. coli O157:H7 ChuS provides evidence for a novel heme degradation activity in a Gram-negative bacterium.

  10. Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 Å resolution by sulfur-SAD phasing.

    Science.gov (United States)

    Fukakusa, Shunsuke; Kawahara, Kazuki; Nakamura, Shota; Iwashita, Takaki; Baba, Seiki; Nishimura, Mitsuhiro; Kobayashi, Yuji; Honda, Takeshi; Iida, Tetsuya; Taniguchi, Tooru; Ohkubo, Tadayasu

    2012-10-01

    CofA, a major pilin subunit of colonization factor antigen III (CFA/III), forms pili that mediate small-intestinal colonization by enterotoxigenic Escherichia coli (ETEC). In this study, the crystal structure of an N-terminally truncated version of CofA was determined by single-wavelength anomalous diffraction (SAD) phasing using five sulfurs in the protein. Given the counterbalance between anomalous signal strength and the undesired X-ray absorption of the solvent, diffraction data were collected at 1.5 Å resolution using synchrotron radiation. These data were sufficient to elucidate the sulfur substructure at 1.38 Å resolution. The low solvent content (29%) of the crystal necessitated that density modification be performed with an additional 0.9 Å resolution data set to reduce the phase error caused by the small sulfur anomalous signal. The CofA structure showed the αβ-fold typical of type IVb pilins and showed high structural homology to that of TcpA for toxin-coregulated pili of Vibrio cholerae, including spatial distribution of key residues critical for pilin self-assembly. A pilus-filament model of CofA was built by computational docking and molecular-dynamics simulation using the previously reported filament model of TcpA as a structural template. This model revealed that the CofA filament surface was highly negatively charged and that a 23-residue-long loop between the α1 and α2 helices filled the gap between the pilin subunits. These characteristics could provide a unique binding epitope for the CFA/III pili of ETEC compared with other type IVb pili.

  11. Study of the resistance mechanisms to ultraviolet light in Escherichia Coli. III. Genetic mapping of a mutation that confers resistance to radiation; Mecanismos de resistencia a luz ultravioleta en Escherichia coli. III. Mapeo genetico de una mutacion que confiere resistencia a radiacion

    Energy Technology Data Exchange (ETDEWEB)

    Alcantara D, D. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    1996-08-15

    Inside this third work with respect to the investigation of the process of adaptation of different subcultures of Escherichia coli, to the UV light, its are present the obtained results in the identification of the gene or genes implied in the resistance to UV of one of the 5 populations derived for repeated exposure to growing dose of UV light of E. coli PQ30. Those obtained data indicate that the causing mutation of the phenotype is located in a single gene, since the insert of the fragment of DNA miniTn5 in this gene, causes the lost one from the resistance and the return to the original phenotype. The gene in question is denominated radA, located in the minute 99.6 of the chromosome of E. coli and required for the efficient repair of double ruptures in the DNA. (Author)

  12. LEITURA E SEUS SUPORTES: papel e digital

    Directory of Open Access Journals (Sweden)

    Celso Leopoldo Pagnan

    2016-09-01

    Full Text Available Pesquisas pioneiras (Mangen; Walgermo; Brønnick, 2012 têm destacado o modo de ler textos curtos ou livros inteiros em dois suportes: digital e papel. Há uma suposição de que os jovens tendem a preferir a leitura digital ao suporte papel, porém pode não ser uma verdade absoluta, conforme atestam outras pesquisas (PewResearch Center, 2014. Com base em dados desses estudos, bem como na aplicação de testes em alunos do ensino médio, o presente trabalho parte do pressuposto de que o nível de percepção dos leitores pode ser relativamente diferente conforme o suporte utilizado. Para tanto, utilizamos como metodologia a aplicação de um teste de leitura em vinte e nove alunos, que leram textos curtos, com um ou dois parágrafos, em um suporte digital, tablet, e responderam a questões de compreensão sobre tais textos; repetindo o procedimento com outros textos em suporte papel. A hipótese era de que os alunos apresentassem modos diferentes de compreensão textual de acordo com o suporte utilizado. A análise dos dados coletados revelou um resultado sutilmente superior na compreensão dos textos impressos, porém insuficiente para ser generalizado como resultado conclusivo, não deixando, entretanto, de apontar caminhos significativos para a compreensão das práticas leitoras na escola.

  13. Structure of the cytoplasmic loop between putative helices II and III of the mannitol permease of Escherichia coli: a tryptophan and 5-fluorotryptophan spectroscopy study.

    Science.gov (United States)

    Vos, Erwin P P; Bokhove, Marcel; Hesp, Ben H; Broos, Jaap

    2009-06-16

    In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EII(mtl), are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be involved in the mannitol translocation process. To spectroscopically distinguish between the Trp positions in each subunit of dimeric EII(mtl), 5-FTrp was biosynthetically incorporated because of its much simpler photophysics compared to those of Trp. The steady-state and time-resolved fluorescence methodologies used point out that all four positions are in structured environments, both in the absence and in the presence of a saturating concentration of mannitol. The fluorescence decay of all 5-FTrp-containing mutants was highly homogeneous, suggesting similar microenvironments for both probes per dimer. However, Stern-Volmer quenching experiments using potassium iodide indicate different solvent accessibilities for the two probes at positions 97 and 133. A 5 A two-dimensional (2D) projection map of the membrane-embedded IIC(mtl) dimer showing 2-fold symmetry is available. The results of this work are in better agreement with a 7 A projection map from a single 2D crystal on which no symmetry was imposed.

  14. PAPEL DO BRIC NA ECONOMIA MUNDIAL

    Directory of Open Access Journals (Sweden)

    Leandro Bruno Santos

    2010-01-01

    Full Text Available Se evalúa el papel de los países BRIC - Brasil, China, India, Rusia y Sudáfrica - en la economía mundial en los primeros años de este siglo. Actualmente, con la crisis fi nanciera internacional y la desaceleración económica en los países desarrollados, algunos analistas han argumentado que el BRIC tendrá un papel importante en el crecimiento económico sostenido de la economía mundial. Se examina cuidadosamente esta hipótesis de independencia del BRIC en relación con las economías desarrolladas, a partir de datos estadísticos, tales como PIB, ingreso per cápita, el comercio, la inversión- extranjera directa, la internacionalización de las empresas, entre otros, obtenidos de las principales organizaciones internacionales como Conferencia de las Naciones Unidas para el Comercio y desarrollo (UNCTAD y Banco Mundial.

  15. El papel de los alimentos en la transmisión de Escherichia coli potencialmente patógenas para el hombre: prevalencia y caracterización de cepas diarreagénicas y productoras de β‐lactamasas de espectro extendido

    OpenAIRE

    Herrera Estévez, Alexandra

    2015-01-01

    El brote del 2011 provocado por el ECVT‐ECEA O104:H4 en Europa marcó un antes y un después en la forma de entender y valorar las infecciones por E. coli diarreagénicos, ya que puso en evidencia el riesgo de que otros serotipos de ECVT pudieran emerger como patógenos importantes para los humanos en el futuro. Hasta esa fecha, la mayoría de los estudios se centraban exclusivamente en el serotipo enterohemorrágico O157:H7 y en la carne de vacuno como principal fuente de transmisión. ...

  16. Estudos de QSAR baseados em dados de atividade biológica obtidos por microcalorimetria: III interação de m-alcoxifenóis e p-hidroxibenzoatos de alquila com Escherichia coli QSAR based on biological microcalorimetry: III interaction of m-alcoxy-phenols and p-hydroxybenzoates with Escherichia coli

    Directory of Open Access Journals (Sweden)

    P. L. O. Volpe

    1997-04-01

    Full Text Available QSAR studies based on flow microcalorimetric bioassay data for interaction of homologous series of m-alkoxyphenols and p-hydroxybenzoates with E. coli cells were carried out applying factorial design. Results for both series showed a linear relationship between log(dosemax and log Po/w. Analysis of these data allows the identification of contributions toward the derived bioactivity from the parent structures (the molecule minus n-CH2 groups present in the side-chain and the lipophilic groups, CH2. These results are discussed with respect to drug quantitative structure-relationship.

  17. El papel del celador en el hospital

    OpenAIRE

    Pérez Alcocer, Alberto

    2015-01-01

    Cartel presentado en la Segunda Conferencia Internacional de Comunicación en Salud, celebrada el 23 de octubre de 2015 en la Universidad Carlos III de Madrid Introducción: el celador es una pieza fundamental , cuando se realiza tanto un ingreso como un alta , ya que es el encargado de llevar el paciente hasta su destino e informarle de las normas del centro hospitalario. Objetivo: dar a conocer las funciones del celador. Metodología: realizamos una búsqueda bibliográfica en Pubmed y...

  18. Escherichia Coli

    Science.gov (United States)

    Goodsell, David S.

    2009-01-01

    Diverse biological data may be used to create illustrations of molecules in their cellular context. I describe the scientific results that support a recent textbook illustration of an "Escherichia coli cell". The image magnifies a portion of the bacterium at one million times, showing the location and form of individual macromolecules. Results…

  19. A putative, novel coli surface antigen 8B (CS8B) of enterotoxigenic Escherichia coli.

    Science.gov (United States)

    Njoroge, Samuel M; Boinett, Christine J; Madé, Laure F; Ouko, Tom T; Fèvre, Eric M; Thomson, Nicholas R; Kariuki, Samuel

    2015-10-01

    Enterotoxigenic Escherichia coli (ETEC) strains harbor multiple fimbriae and pili to mediate host colonization, including the type IVb pilus, colonization factor antigen III (CFA/III). Not all colonization factors are well characterized or known in toxin positive ETEC isolates, which may have an impact identifying ETEC isolates based on molecular screening of these biomarkers. We describe a novel coli surface antigen (CS) 8 subtype B (CS8B), a family of CFA/III pilus, in a toxin producing ETEC isolate from a Kenyan collection. In highlighting the existence of this putative CS, we provide the sequence and specific primers, which can be used alongside other ETEC primers previously described.

  20. El despegue industrial y el papel de los empresarios

    Directory of Open Access Journals (Sweden)

    Luis Aurelio Ordoñez B.

    1997-02-01

    Full Text Available RESUMEN Analiza a la luz de la historiografía económica colombiana, cuáles han sido algunas de las características más ssobresalientes del devenir empresarial en Colombia y el papel que los empresarios han desempeñado en el despegue industrial colombiano.

  1. Occurrence of Escherichia coli in Brassica rapa L. chinensis ...

    African Journals Online (AJOL)

    Administrator

    2016-12-07

    Dec 7, 2016 ... Although there is no established criteria for concentration of E. coli in the .... Risk Assessment Series No. 14. Rome. Mhongole et al. 2777. ICMSF (1986). ... of Wastewater, Excreta and Greywater: Volume III - Wastewater and.

  2. Identificación de péptidos bloqueantes del sistema de secreción de tipo III y de la adherencia a epitelios de Escherichia coli enterohemorrágico (EHEC) y Escherichia coli enteropatógeno (EPEC)

    OpenAIRE

    Larzábal, Mariano

    2010-01-01

    Escherichia coli Enteropatógeno (EPEC) y Escherichia coli Enterohemorrágico (EHEC) se encuentran asociadas con diarrea en seres humanos. EPEC es una de las principales causas de diarrea infantil en países desarrollados, mientras que EHEC es responsable de enfermedades cuyo espectro clínico incluye diarrea, colitis hemorrágica y síndrome urémico hemolítico (SUH), que es la principal causa de insuficiencia renal aguda de niños menores de 5 años en la Argentina. La expresión de la toxina Shiga (...

  3. La doble historia de El convidado de papel

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    Ceballos Viro, Álvaro

    2011-12-01

    Full Text Available This article sets out to question the textual history of El convidado de papel, a novel by Benjamín Jarnés. The differences between the two firsts editions of this novel, published in 1928 and 1935, are more complex than has hitherto been assumed. They also allow to reconstruct a different plot from the one that can be found in literary histories. In its original version, El convidado de papel was not only a peculiar Bildungsroman, but also a typical doppelgänger narrative. Therefore, it contained a dense web of intertextual references which Jarnés deliberately eliminated in the second edition of the novel. These intertextual references stimulate the search for new sources as well as the re-evaluation of the precise mode of influence attributed to familiar sources.La presente contribución comienza problematizando la historia textual de El convidado de papel, de Benjamín Jarnés. Las diferencias entre las dos primeras ediciones, que datan de 1928 y 1935, no sólo son más complejas de lo que hasta ahora se había creído, sino que además permiten reconstruir un argumento diferente de aquél tácitamente consensuado por la historiografía literaria. Sin dejar de ser una peculiar novela de formación, El convidado de papel era en su versión original una novela de doble en toda regla, lo que la abría a unas relaciones intertextuales que Jarnés eliminó conscientemente en la segunda edición. Tales relaciones permiten, por un lado, sugerir nuevas influencias (o modos de influencia en la narrativa jarnesiana y, por otro lado, profundizar en las deudas contraídas con autores cuyo ascendiente ya era conocido por la crítica.

  4. Periodismo y ciclismo. Perspectivas del papel organizador de la prensa

    Directory of Open Access Journals (Sweden)

    Juan Francisco de la Cruz Moreno

    2002-01-01

    Full Text Available El presente artículo versa sobre el papel que la prensa ha ejercido en la creación y organización de competiciones deportivas, especialmente dentro del ciclismo. Las principales carreras del calendario cuentan con un origen periodístico motivado fundamentalmente por intereses económicos y, en algunos casos, también políticos.

  5. O papel da ONU no conflito do Saara Ocidental

    Directory of Open Access Journals (Sweden)

    Geraldine Rosas Duarte

    2016-03-01

    Full Text Available Em abril de 2015 o mandato da MINURSO foi mais uma vez prorrogado, embora não haja indícios de que a questão sarauí esteja caminhando rumo à resolução. O artigo analisa o papel da ONU no conflito refletindo sobre os limitados avanços alcançados por meio da intervenção.

  6. Estudos de QSAR baseados em dados de atividade biológica obtidos por microcalorimetria: III interação de m-alcoxifenóis e p-hidroxibenzoatos de alquila com Escherichia coli

    OpenAIRE

    Volpe,P.L.O.; Montanari,C. A.

    1997-01-01

    QSAR studies based on flow microcalorimetric bioassay data for interaction of homologous series of m-alkoxyphenols and p-hydroxybenzoates with E. coli cells were carried out applying factorial design. Results for both series showed a linear relationship between log(dose)max and log Po/w. Analysis of these data allows the identification of contributions toward the derived bioactivity from the parent structures (the molecule minus n-CH2 groups present in the side-chain) and the lipophilic group...

  7. Richard III

    DEFF Research Database (Denmark)

    Lauridsen, Palle Schantz

    2017-01-01

    Kort analyse af Shakespeares Richard III med fokus på, hvordan denne skurk fremstilles, så tilskuere (og læsere) langt henad vejen kan føle sympati med ham. Med paralleller til Netflix-serien "House of Cards"......Kort analyse af Shakespeares Richard III med fokus på, hvordan denne skurk fremstilles, så tilskuere (og læsere) langt henad vejen kan føle sympati med ham. Med paralleller til Netflix-serien "House of Cards"...

  8. Estudios taxonómicos, ontogenéticos, ecológicos y etológicos sobre los Ostrácodos de agua dulce en Colombia- III. El Prenauplio y su papel en la eclosión del huevo en el género Chlamydotheca Saussure 1858 (Ostrácoda, Podocopa, Cyprididae Estudios taxonómicos, ontogenéticos, ecológicos y etológicos sobre los Ostrácodos de agua dulce en Colombia- III. El Prenauplio y su papel en la eclosión del huevo en el género Chlamydotheca Saussure 1858 (Ostrácoda, Podocopa, Cyprididae Estudios taxonómicos, ontogenéticos, ecológicos y etológicos sobre los Ostrácodos de agua dulce en Colombia- III. El Prenauplio y su papel en la eclosión del huevo en el género Chlamydotheca Saussure 1858 (Ostrácoda, Podocopa, Cyprididae

    Directory of Open Access Journals (Sweden)

    Roessler Ewald W.

    1982-12-01

    Full Text Available El estudio describe el exoesqueleto prenauplial de algunas especies del género Chlamydotheca y hace énfasis en la presentación de la estructura funcionalde este mismo, respecto a su papel en los procesos que causan la eclosión.  Se comprueba la existencia de dos períodos en el desarrollo embrionario que muestran actividad osmótica, induciendo la segunda fase, la eclosión, y subsiguientemente el último paso de la primera muda.The study describes the exoskeleton of the pronauplius of some species of the genus Chiamydotheca with special reference to its functional structure and its role in the hatching processes. Two periods of the late embriological development will be described that show osmotic activity, the latter primarily causes hatching and finally terminates the first molting process.In der vorliegenden Studie wird das pronaupliale Exoskelett einiger Arten der Gattung Chlamydotheca beschrieben unter besonderer Beruecksichtigung seiner Funktionsstruktur im Hinblick auf den Schlupfprozess.  Es werden zwei Perioden der spaeten Embryonalentwicklung vorgestellt, die sich durch starke osmotische Aktivitaet auszeichnen und von denen die zweite zunaechst den Schlupfprozess verursacht und danach die erste Haeutung abschliesst.

  9. Papel del gerente lder venezolano: Gerencia vs. Liderazgo

    Directory of Open Access Journals (Sweden)

    Gerardo E. Romero

    2005-01-01

    Full Text Available En este artculo se comparan diversas orientaciones de las ciencias gerenciales acerca del papel que juega en la actualidad el gerente-lder venezolano mediante un anlisis explicativo de los aspectos que complejizan la aplicacin de las concepciones de gerencia y liderazgo, describiendo su diferencias y la manera cmo se presentan en las organizaciones venezolanas. Asimismo, se exponen algunos enfoques caractersticos que definen a la gerencia y el liderazgo en los ltimos tiempos, los cuales deben ser asumidos por el nuevo gerente-lder frente a sus necesidades existenciales.

  10. El papel de las redes sociales en los movimientos sociales

    OpenAIRE

    Arrate Cobo, Aroa

    2016-01-01

    Las Redes Sociales cada vez están mas presentes en nuestras vidas, marcando un antes y un después con su llegada. Teniendo esto en cuenta, en este Trabajo de Fin de Grado, se propone un análisis sobre el papel de las redes sociales en los Movimientos Sociales. Para ello se ha realizado una investigación de algunos de los últimos movimientos sociales más presentes en las redes. Esta se completa con entrevistas a participantes de estos movimientos, al objeto de contrastar los datos ...

  11. Publicidad sexista. El papel de la mujer en publicidad

    OpenAIRE

    María Rodríguez, Laura

    2013-01-01

    Proyecto Fin de Grado leído en la Universidad Rey Juan Carlos en el curso académico 2013/2014. Director: María Sánchez Hernández El estudio realizado trata sobre el discurso sexista que continua presente en la publicidad española. Analizamos los diferentes estereotipos y roles que se asocian a cada género y el papel de la mujer en los anuncios emitidos por televisión. La investigación recoge estudios relacionados cuyos resultados reflejan el alto contenido sexista Ciencias de la Comunic...

  12. El deporte escolar en Asturias y su papel educativo

    OpenAIRE

    Díaz Ordóñez, Rolando

    2015-01-01

    La presente investigación El deporte escolar en Asturias y su papel educativo, supone la continuación de una línea de investigación abierta en el año 2004 con el trabajo de investigación desarrollado para obtener la suficiencia investigadora titulado: El deporte extraescolar en Asturias. Deporte, ocio o competición. Análisis de una Comarca. La Tesis Doctoral se presenta en tres partes claramente diferenciadas: I. Fundamentación teórica: Capítulo 1. Deporte Escolar Capítulo 2. Di...

  13. Papel pericial de las matemáticas. Los repartos

    OpenAIRE

    Gámiz, Lina; Pablo MARTÍNEZ

    1996-01-01

    Con objeto de contextualizar la proporcionalidad, hemos planteado en diversos niveles de enseñanza de las matemáticas, el clásico problema de la herencia de camellos. En este articulo presentamos la solución y las reflexiones que ha realizado sobre el problema una estudiante para profesora de matemáticas de secundaria. Estas reflexiones nos han permitido profundizar sobre el significado matemático de reparto y sobre el papel pericial de las matemáticas.

  14. Vida como drama: repensando o papel do cliente em psicoterapia

    OpenAIRE

    Eloisa Nogueira Aguiar

    2011-01-01

    O artigo busca uma discussão sobre o papel do cliente em psicoterápica, a partir do pensamento de José Ortega y Gasset. Se a psicoterapia é a preocupação com a experiência vivencial, sua filosofia se oferece de grande valia, pois coloca a vida humana no centro de suas investigações. A vida é um projeto pessoal que implica, simultaneamente, as circunstâncias e a subjetividade. A vida requer um processo de “quefazer”. Viver é sentir-se fatalmente forçado a exercitar a liberdade, a decidir quem ...

  15. Papel social e paradigmas da pesquisa qualitativa de enfermagem

    Directory of Open Access Journals (Sweden)

    Maria Ribeiro Lacerda

    2011-04-01

    Full Text Available Pesquisa bibliográfica realizada na Revista Brasileira de Enfermagem com delimitação temporal de 2005 a 2008, que compreendeu 24 fascículos presentes na base de dados SciELO. O objetivo foi identificar temas emergentes nas pesquisas qualitativas publicadas em periódico de enfermagem; correlacionar os temas com a produção do conhecimento da enfermagem e refletir sobre o papel social das pesquisas qualitativas analisadas. Identificaram-se 173 estudos qualitativos que após análise resultaram nas categorias: formação em enfermagem, áreas de atuação, base/fundamentos/concepções de cuidado de enfermagem e assuntos profissionais. Na relação entre as categorias, padrões de conhecimento e papel social destacam o conhecimento sóciopolítico, apontando um amadurecimento da profissão em relação às questões sociais intrínsecas, parte do seu corpo social, e extrínsecas que são as demandas da sociedade.

  16. Vida como drama: repensando o papel do cliente em psicoterapia

    Directory of Open Access Journals (Sweden)

    Eloisa Nogueira Aguiar

    2011-08-01

    Full Text Available O artigo busca uma discussão sobre o papel do cliente em psicoterápica, a partir do pensamento de José Ortega y Gasset. Se a psicoterapia é a preocupação com a experiência vivencial, sua filosofia se oferece de grande valia, pois coloca a vida humana no centro de suas investigações. A vida é um projeto pessoal que implica, simultaneamente, as circunstâncias e a subjetividade. A vida requer um processo de “quefazer”. Viver é sentir-se fatalmente forçado a exercitar a liberdade, a decidir quem vai ser neste mundo. Este é o caráter dramático da vida. Esta visão ajuda a explicar a sua negação afiada de uma essência humana. Assim, altera-se o papel do cliente, bem como sua expectativa em relação ao psicólogo.

  17. E. Coli and Pregnancy

    Science.gov (United States)

    ... best live chat Live Help Fact Sheets Share E. coli and Pregnancy Thursday, 20 November 2014 In every ... risk. This sheet talks about whether exposure to E. coli may increase the risk for birth defects over ...

  18. E. coli enteritis

    Science.gov (United States)

    ... medlineplus.gov/ency/article/000296.htm E. coli enteritis To use the sharing features on this page, please enable JavaScript. E. coli enteritis is swelling (inflammation) of the small intestine from ...

  19. E. Coli Infections

    Science.gov (United States)

    E. coli is the name of a type of bacteria that lives in your intestines. Most types of E. coli are harmless. However, some types can make you ... type causes travelers' diarrhea. The worst type of E. coli causes bloody diarrhea, and can sometimes cause kidney ...

  20. Papel del factor tumoral en el control de las reservas grasas y la obesidad.

    OpenAIRE

    M Bullo Bonet; P Garcia-Lorda; JM Argilés; J Salas Salvado

    2000-01-01

    Papel del factor tumoral en el control de las reservas grasas y la obesidad. The role of tumor necrosis factor in the control of fat reserve and obesity. Papel del factor tumoral en el control de las reservas grasas y la obesidad. The role of tumor necrosis factor in the control of fat reserve and obesity.

  1. O papel do sistema dopaminérgico na fobia social

    Directory of Open Access Journals (Sweden)

    Kauer-SantAnna Márcia

    2002-01-01

    Full Text Available Os autores descrevem o papel do sistema dopaminérgico na fobia social. Evidências que indicam disfunção dopaminérgica são descritas, e o papel da dopamina como um regulador da atividade social é discutido.

  2. Papel del estrés oxidativo en la esquizofrenia

    Directory of Open Access Journals (Sweden)

    María Clara Soto-Bernardini

    2008-12-01

    Full Text Available La esquizofrenia es una enfermedad mental severa que se presenta en alrededor del 1% de la población mundial. Para que se manifieste son necesarios tanto factores genéticos como ambientales, por lo que el genotipo de riesgo no conlleva al desarrollo de la enfermedad sino a una mayor susceptibilidad de presentarla. Existe evidencia de que el balance entre la producción de radicales libres y el sistema de defensa antioxidante está alterado en pacientes con esquizofrenia. Sin embargo, no se sabe con certeza si la producción de RLs tiene un papel causal en el desarrollo de la enfermedad o es producido durante el curso de la enfermedad, o por el uso de neurolépticos. En este artículo se revisa la evidencia de la participación de estas sustancias en el desarrollo de la enfermedad.

  3. O papel do cinema no ensino de filosofia

    OpenAIRE

    2014-01-01

    Relatório de estágio de mestrado em Ensino de Filosofia no Ensino Secundário O presente Relatório de Estágio pretende ser um registo e uma avaliação da implementação, nas aulas de filosofia da turma do 10ºAV1 da Escola Secundária Martins Sarmento, em Guimarães, do Plano de Intervenção Pedagógica intitulado “O papel do cinema no ensino de filosofia”, concebido no âmbito do estágio profissional do curso de Mestrado em Ensino de Filosofia no Ensino Secundário, da Universidade do M...

  4. El papel de la universidad en Comunidades de Aprendizaje

    Directory of Open Access Journals (Sweden)

    Antonio Aguilera

    2010-01-01

    Full Text Available En este artículo se describen y analizan las existentes y posibles relaciones entre la universidad y las Comunidades de Aprendizaje, así como los beneficios para ambas que se derivan de la colaboración entre ellas. Para ello nos centramos en el papel que la universidad puede desempeñar en los procesos de transformación en Comunidades de Aprendizaje que se están llevando a cabo en diferentes centros educativos. Se exponen las experiencias de las universidades de Barcelona y Sevilla. Se sugiere una segunda vía de colaboración que tiene que ver con la transformación de los propios centros universitarios en Comunidades de Aprendizaje.

  5. Tomo III

    OpenAIRE

    2015-01-01

    Memorias, histórico, físicas, crítico, apologéticas de la América Meridional con unas breves advertencias y noticias útiles, a los que de orden de Su Majestad, hubiesen de viajar y describir aquellas vastas regiones. Reino Animal. Tomo III. Por un anónimo americano en Cádiz por los años de 1757. Primera Parte Prólogo Artículo 1°De los cuadrúpedos útiles al hombre a varios usos y a su sustento. Vaca Caballos Carneros de la tierra, especie de camellos Vicuña Guanacos Puercos monteses Artículo 2...

  6. Escherichia coli isolated from feces of brown bears (Ursus arctos) have a lower prevalence of human extraintestinal pathogenic E. coli virulence-associated genes.

    Science.gov (United States)

    Vadnov, Maruša; Barbič, Damjana; Žgur-Bertok, Darja; Erjavec, Marjanca Starčič

    2017-01-01

    Eighty-six Escherichia coli strains from feces of either wild brown bears or those living in a zoo were screened for phylogenetic groups using the revisited Clermont phylotyping method and the prevalence of 24 virulence-associated genes (VAGs) of extraintestinal pathogenic E. coli (ExPEC). Our results showed that most strains of E. coli in bears belonged to phylogenetic groups III/IV/V (29%) and B1 (26%). Only half of the tested VAGs were found in the E. coli bear strains, with fimH present in 72%, ompT in 63%, and kpsMT in 43% of the strains. When the data obtained on the fecal E. coli strains from brown bears were compared with the data obtained on 90 fecal E. coli strains from healthy humans, there were significant differences in E. coli population structures between both hosts.

  7. O papel dos intelectuais na América Latina

    Directory of Open Access Journals (Sweden)

    Elzimar Goettenauer de Marins Costa

    2011-11-01

    Full Text Available Resumo: O intelectual detém um saber; ele possui conhecimentos que, ademais de habilitá-lo profissionalmente, podem capacitá-lo a exercer uma determinada função não necessariamente restrita à sua profissão. O desafio será sempre o de não perder de vista os valores – a ética – do papel que é convocado a desempenhar. Ética que deriva do respeito às necessidades essenciais do ser humano: viver plena e dignamente, em um ambiente onde não só esteja garantido o acesso às condições materiais de existência, como também estejam asseguradas a liberdade e a justiça. Norteando por esses princípios, o intelectual na América Latina tem sido impelido a discutir, esclarecer e denunciar o que impede a plenitude da vida humana.Palavras-chave: Sociologia; crítica literária; intelectual; América Latina.Resumen: El intelectual detiene un saber, posee conocimientos que, además de autorizarlo profesionalmente, pueden habilitarlo a ejercer determinada función no siempre restricta a su profesión. Su reto será el de tener presentes los valores – la ética – del papel que debe desempeñar. Ética que deriva del respeto a las necesidades esenciales del ser humano: vivir de modo pleno y digno, en un ambiente donde se garantice el acceso a las condiciones materiales de existencia y se aseguren la libertad y la justicia. Anclando en dichos princípios, el intelectual en América Latina se ha dedicado a discutir, aclarar y denunciar lo que impide la plenitude de la vida humana.Palabras-clave: Sociología; crítica literaria; intelectual; América Latina.Keywords: Sociology; literary criticism; intelectual; Latin America.

  8. Papel de los seguidores en el desarrollo de las teorías de liderazgo organizacional

    OpenAIRE

    Mónica García Solarte

    2015-01-01

    La mayoría de revisiones acerca del liderazgo se han enfocado en el líder y su papel dentro de la organización. Las investigaciones actuales demuestran que los seguidores, como un elemento importante del liderazgo, poseen un papel activo en la selección del estilo de liderazgo y en la teoría desde la cual se le evaluará a un líder. Desde este punto, el objetivo de este artículo es identificar cuál ha sido el papel de los seguidores en el desarrollo de las teorías del liderazgo organizacional....

  9. Papel de la miostatina en la regulación de la hematopoyesis

    OpenAIRE

    2012-01-01

    La miostatina (MSTN) es un miembro de la superfamilia del TGF- , que se expresa preferentemente en el músculo esquelético inhibiendo el crecimiento muscular. Aunque el papel fundamental de la MSTN es la regulación de la masa muscular también puede jugar un papel en otros tejidos como músculo cardíaco, tejido adiposo, glándula mamaria, hueso, cerebro, útero y testículos o células hematopoyéticas. En el presente estudio se ha analizado el papel de la MSTN en la regulación de ...

  10. O papel da música nas atividades de trabalho

    Directory of Open Access Journals (Sweden)

    Ana Raquel Motta

    2015-07-01

    Full Text Available O artigo discute o papel da música no trabalho, considerando a atividade musical como uma prática discursiva intersemiótica. Para ser capaz de examinar o tema tanto do ponto de vista da música quanto do trabalho, colocados juntos pelo discurso, a abordagem utilizada é interdisciplinar, composta por análise do discurso, etnomusicologia e ergologia. Primeiramente as fronteiras do conceito tradicional de cantos de trabalho são expandidas através da análise de dois exemplos pouco convencionais: uma peça publicitária e uma embalagem de produto. A partir desse viés expandido, o artigo explora cantos de duas comunidades brasileiras, da Bahia e do Maranhão, que cantam enquanto trabalham em atividades agrícolas e extrativistas. A conclusão ressalta que a relação entre música e trabalho ultrapassa em muito a abordagem estreita dos cantos de trabalho. Para compreendê-la, é preciso levar em conta as renormalizações postas em jogo e as comunidades discursivas implicadas por estas práticas discursivas complexas.

  11. PAPEL ACTIVO DEL LENGUAJE EN LA PRAXIS DEL HOMBRE

    OpenAIRE

    RICARDO PÉREZ GUZMÁN

    2014-01-01

    Las conclusiones teóricas de Adam Schaff sobre el papel activo del lenguaj en sus relaciones con cl pensamiento, el conocimiento y la cultura, se apoyan en reflexiones argumentadas desde el punto de vista e los análisis filosóficos del lenguaje. Al análisis de estas relaciones llega el autor después de plantear la historia del problema en los tratados filosóficos, para analizar luego las premisas empíricas de la hipótesis Sapir - Worf que le permiten un acercamiento a los aspectos fundamental...

  12. Papeles peligrosos. Sobre la introducción de libros, papeles e impresos sediciosos que perturban la quietud de los pueblos con sus ideas perniciosas

    Directory of Open Access Journals (Sweden)

    Paula Ronderos

    2014-12-01

    Full Text Available Libros, papeles e impresos de a pliego o en cuarto, octavo, folio; notas, consignas, quejas. Emancipatorias. Personales. Funerarias. Ideas de libertad y de sedición, de derrocamiento. Versiones contrarias de asuntos locales que circulaban de mano en mano, pegados en muros y columnas, postes y puertas. Papeles peligrosos e incendia rios, virulentos. Perseguidosy borrados. Reducidos a la mínima expresión. Durante el periodo colonial los castigos se normaron severos para quien escribía papeles y también eran “incursos todos los que las oyeren leer, o supieren que existen en poder de alguna persona, a quien tendrán la obligación de denunciar” (Amar, 1809.

  13. SERÁ O FIM DO PAPEL? OS AVANÇOS TECNOLÓGICOS E SEUS POSSÍVEIS IMPACTOS NO CONSUMO DE PAPEL

    Directory of Open Access Journals (Sweden)

    Fernando de Souza Meirelles

    2011-07-01

    Full Text Available O presente trabalho traçou estudos estatísticos entre o aumento do uso de Tecnologia de Informação (TI e o consumo de papel no mercado brasileiro, utilizando dados de vinte anos. Por meio da revisão bibliográfica e testes estatísticos, foi investigado se os investimentos corporativos em TI proporcionaram a redução do consumo de determinados tipos de papéis. Os resultados corroboram estudo anterior, desenvolvido por Moraes, Meirelles e Nacimiento (2010, no qual foi analisado um período de seis anos, indicando que o uso de TI não trouxe como benefício a redução do consumo de papel. Evidencia-se que os valores de investimentos em TI pelas empresas obtiveram correlação positiva com a queda no consumo de papel do tipo imprensa e escrita, indicando que, possivelmente, o acesso digital às informações reduziu a utilização de jornais impressos. Percebe-se também o aumento no consumo de outros tipos de papel, como o papel de impressão e embalagem, juntamente com os investimentos em TI. Dessa forma, pode-se deduzir que o fácil acesso a equipamentos de informática levaria à impressão de arquivos eletrônicos, bem como o aumento das vendas de equipamentos eletrônicos tenderia a aumentar a utilização do papel de embalagem.

  14. ANALISIS CEMARAN BAKTERI Escherichia coli ANALISIS CEMARAN BAKTERI Escherichia coli ANALISIS CEMARAN BAKTERI Escherichia coli

    OpenAIRE

    ANGGREINI, RAHAYU

    2015-01-01

    2015 RAHAYU ANGGREINI coli Penelitian ini bertujuan untuk melakukan identifikasi cemaran bakteri E. coli O157:H7 pada daging sapi di kota Makassar. Sampel pada penelitian ini sebanyak 72 sampel Kata Kunci : Daging sapi, pasar tradisional, E. coli, E. coli O157:H7, kontaminasi bakteri, identifikasi E. coli O157:H7.

  15. ANALISIS CEMARAN BAKTERI Escherichia coli ANALISIS CEMARAN BAKTERI Escherichia coli ANALISIS CEMARAN BAKTERI Escherichia coli

    OpenAIRE

    ANGGREINI, RAHAYU

    2015-01-01

    2015 RAHAYU ANGGREINI coli Penelitian ini bertujuan untuk melakukan identifikasi cemaran bakteri E. coli O157:H7 pada daging sapi di kota Makassar. Sampel pada penelitian ini sebanyak 72 sampel Kata Kunci : Daging sapi, pasar tradisional, E. coli, E. coli O157:H7, kontaminasi bakteri, identifikasi E. coli O157:H7.

  16. Zoonotic Escherichia coli

    Directory of Open Access Journals (Sweden)

    Wasteson Yngvild

    2002-03-01

    Full Text Available Escherichia coli is a normal inhabitant of the gastrointestinal tract of all warm-blooded animals, but variants of this species is also among the important etiological agents of enteritis and several extraintestinal diseases. The E. coli strains that cause diarrhoeal illness are categorised into pathogenicity groups based on virulence properties, mechanisms of pathogenicity, clinical symptoms and serology. The five main categories include enterotoxinogenic E. coli (ETEC, enteropathogenic E. coli (EPEC, enteroaggregative E. coli (EAggEC, enteroinvasive E. coli (EIEC and Shiga (Vero toxin-producing E. coli (STEC/VTEC. From a zoonotic point of view, STEC is the only E. coli pathogenicity group of major interest, as the shiga toxin-producing strains are able to cause severe disease in humans when being transmitted through the food chain from their animal reservoirs. The focus of this manuscript is therefore on STEC; pathogenicity factors, disease, the reservoirs and on-farm ecology, transmission into the food chain, growth and survival in food and in the environment, and the shiga toxin-encoding bacteriophages.

  17. PATHOGENIC ESCHERICHIA COLI

    Science.gov (United States)

    Escherichia coli is a bacterial species which inhabits the gastrointestinal tract of man and warm-blooded animals. Because of the ubiquity of this bacterium in the intestinal flora, it serves as an important indicator organism of fecal contamination. E. coli, aside from serving a...

  18. Diarrheagenic Escherichia coli.

    Science.gov (United States)

    Gomes, Tânia A T; Elias, Waldir P; Scaletsky, Isabel C A; Guth, Beatriz E C; Rodrigues, Juliana F; Piazza, Roxane M F; Ferreira, Luís C S; Martinez, Marina B

    2016-12-01

    Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are collectively known as diarrheagenic E. coli. In this review, we have gathered information on current definitions, serotypes, lineages, virulence mechanisms, epidemiology, and diagnosis of the major diarrheagenic E. coli pathotypes.

  19. Engineering E. coli-E. coli cocultures for production of muconic acid from glycerol

    National Research Council Canada - National Science Library

    Zhang, Haoran; Li, Zhengjun; Pereira, Brian; Stephanopoulos, Gregory

    2015-01-01

    .... In this study, we aim to explore the potential of the E. coli-E. coli coculture system to use a single renewable carbon source, glycerol, for the production of value-added product cis, cis-muconic acid...

  20. El papel del telespectador en los medios audiovisuales

    Directory of Open Access Journals (Sweden)

    Gloria Rosique Cedillo

    2012-04-01

    Full Text Available Sin lugar a duda, el papel del telespectador ha tomado un matiz radicalmente distinto al que prevaleció durante décadas con la televisión analógica. A raíz de la entrada y convergencia con las nuevas tecnologías, el usuario ha podido comprobar su independencia creando su propia parrilla de programación, con opción a poder visionarla en cualquier momento, así como de experimentar una nueva faceta como productor y distribuidor de sus propios contenidos por Internet.Como resultado de la fusión de las tecnologías, se han hecho presentes nuevas herramientas de comunicación donde, si bien el espacio-tiempo ha dejado de ser prioritario, el poder de la imagen se ha perpetuado.Por otra parte la palabra escrita, que se encontraba eclipsada hasta hace unos años por el imperio de la imagen, ha resurgido a través de formas de expresión públicas como los llamados diarios electrónicos, mejor conocidos como ‘blogs’.Hoy en día el usuario de los medios ha podido comprobar las bondades de estas nuevas formas de comunicación pero también el uso amoral que el hombre puede hacer de ellas .En definitiva, la recepción audiovisual, el valor de los contenidos y el de la imagen en sí misma, han devenido y transformado al telespectador analógico en un usuario polivalente de los medios audiovisuales.

  1. PAPEL DEL DOCENTE EN LOS ENTORNOS VIRTUALES DE APRENDIZAJE

    Directory of Open Access Journals (Sweden)

    Liuska Martínez Noris

    2014-08-01

    Full Text Available El presente trabajo aborda la necesidad del empleo de las Tecnologías de la Información y la Comunicación (TIC para un mejor desempeño en la actividad docente-educativa. La formación en entornos virtuales de aprendizaje constituye un importante tema de investigación en propuestas de innovación para la educación superior actual. Los resultados de esta investigación permiten identificar las diversas potencialidades de los usos pedagógicos y didácticos de las TIC como herramientas valiosas en los programas de formación. El papel del docente es fundamental al utilizar las TIC de manera creativa. Se deben adquirir habilidades y destrezas para orientar correctamente el aprendizaje de los estudiantes, estimulando así, la resolución de problemas y la construcción colaborativa del conocimiento.PALABRAS CLAVE: Tecnologías de la Información y la Comunicación; TIC; entornos virtuales de aprendizaje; construcción del conocimientoROLE OF TEACHERS IN VIRTUAL ENVIRONMENTS OF LEARNING  ABSTRACTThe present work approaches the necessity of the employment of the Information and Communication Technologies (ICT for a better acting in the educational-educational activity. The formation in virtual environments of learning constitutes an important investigation topic in innovation proposals for the current superior education. The results of this investigation allow to identify the diverse potentialities of the pedagogic and didactic uses of the TIC like valuable tools in the formation programs. The role of teachers is fundamental when using the ICT in a creative way. Abilities and competences should be acquired to guide the learning of the students correctly, stimulating this way, the resolution of problems and the collaborative construction of the knowledge. KEYWORDS: Information and Communication Technologies; ITC; virtual environments of learning; construction of knowledge

  2. PAPEL ACTIVO DEL LENGUAJE EN LA PRAXIS DEL HOMBRE

    Directory of Open Access Journals (Sweden)

    RICARDO PÉREZ GUZMÁN

    2014-11-01

    Full Text Available Las conclusiones teóricas de Adam Schaff sobre el papel activo del lenguaj en sus relaciones con cl pensamiento, el conocimiento y la cultura, se apoyan en reflexiones argumentadas desde el punto de vista e los análisis filosóficos del lenguaje. Al análisis de estas relaciones llega el autor después de plantear la historia del problema en los tratados filosóficos, para analizar luego las premisas empíricas de la hipótesis Sapir - Worf que le permiten un acercamiento a los aspectos fundamentales de las relaciones entre el lenguaje y el proceso de cognición. El material que recoge el desarrollo de este proceso conceptual se reúne en el volumen Lenguaje y Conocimiento (1, cuyo último tratado “Lenguaje, conocimiento y cultura” se reseña a continuación.

  3. El papel del agua para consumo humano en los brotes de diarrea reportados en el período 1999 - 2001 en Costa Rica

    Directory of Open Access Journals (Sweden)

    Carmen Valiente

    2002-07-01

    Full Text Available El presente estudio recopila los resultados de veintidos brotes de diarrea reportados en Costa Rica, durante el período comprendido entre marzo 1999 y marzo del 2001, con el objetivo de evaluar el papel del agua para consumo humano como canal de transmisión de los potenciales agentes causantes; para lograrlo se estableció un "Sistema Cualitativo de Valoración del Grado de Importancia del Agua para Consumo Humano en la Transmisión de las Diarreas". Los resultados indican que cinco brotes (22.7% se clasifican en la categoría 1, es decir, el agua tuvo un 100% de responsabilidad en la transmisión del agente etiológico; ya que este se aisló tanto en las heces de los pacientes como en el agua para consumo de las poblaciones respectivas. Dos brotes se ubicaron en la categoría 11 (9.1 %; en este caso, aunque se aislaron los mismos géneros bacterianos en las heces y el agua, diferían en la especie o en alguna otra característica. En diez brotes no se logró aislar el agente patógeno en el agua de consumo (45.0%; sin embargo, las poblaciones afectadas utilizaban agua de calidad no potable, por eso se sitúan en la categoría III. En la categoría IV se clasificó solo un brote (4.5%, ya que no se pudo comprobar la presencia del agente patógeno en el agua de consumo y esta no presentaba contaminación fecal (catalogada como potable, pero se aislaron bacterias patógenas oportunistas en las fuentes de abastecimiento. En la categoría V se ubican cuatro brotes (18.2%, pues las poblaciones afectadas usaban agua potable pero no se logró aislar ningún patógeno oportunista en el preciado líquido. Otro aporte de este trabajo es que demuestra que la mayoría de los brotes de diarrea se presentan en la época de verano, principalmente en los meses de febrero y marzo. Además, en el 36.0% de los brotes se aisló la bacteria Shigella (flexneri y sonnei, seguida por Aeromonas (hydrophila y caviae en el 14%. En uno de los brotes se aisló Salmonella

  4. Dexmedetomidina: papel atual em anestesia e cuidados intensivos

    Directory of Open Access Journals (Sweden)

    Joana Afonso

    2012-02-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: Atualizar e rever a aplicação da dexmedetomidina em anestesia e cuidados intensivos. Este trabalho é uma revisão abrangente dos usos clínicos, farmacologia, farmacocinética, mecanismo de ação e efeitos adversos da dexmedetomidina. CONTEÚDO: O uso efetivo de agentes sedativo-hipnóticos e analgésicos é parte integral do conforto e segurança dos pacientes. A dexmedetomidina é um potente e altamente seletivo agonista dos adrenoceptores α-2 com propriedades simpaticolíticas, sedativas, amnésicas e analgésicas, que tem sido descrito como um adjuvante útil e seguro em várias aplicações clínicas. Proporciona uma "sedação consciente" única, analgésica, sem depressão respiratória. Os usos correntes revistos incluem sedação na unidade de tratamento intensivo - UTI (adulta e pediátrica, departamento de emergência, anestesia regional e geral, neurocirurgia, sedação para procedimentos pediátricos, intubação por fibra ótica em doentes acordados, cirurgia cardíaca e cirurgia bariátrica. CONCLUSÕES: A dexmedetomidina oferece a capacidade única de fornecer sedação e analgesia sem depressão respiratória. É um agente novo com uma grande margem de segurança, excelente capacidade sedativa e moderadas propriedades analgésicas. Embora o seu amplo uso seja, atualmente, em pacientes de unidades de cuidados intensivos cirúrgicos e não cirúrgicos, a dexmedetomidina parece ter futuras aplicações promissoras nas áreas de neuroproteção, cardioproteção e renoproteção. São necessários mais estudos detalhados para definir o seu papel como sedativo em doentes críticos, neurocirúrgicos e pediátricos, como adjuvante da anestesia e como sedativo durante procedimentos

  5. PAPEL DE LA CONFIANZA en las relaciones entre organizaciones

    Directory of Open Access Journals (Sweden)

    Irene Juana Guillén Mondragón

    2006-07-01

    Full Text Available El objetivo del presente documento es analizar las definiciones y la importancia de la confianza para crear relaciones de cooperación entre organizaciones de naturaleza distinta. Se hace referencia a los modelos de organización dominantes para acercarnos a los nuevos mecanismos de regulación social propuestos por la sociología para tratar la organización, debido a que en el contexto actual las nuevas concepciones de la organización del trabajo han promovido en las organizaciones formas distintas de competir en el mercado. Procesos de cambio que obligan a las organizaciones a construir una nueva identidad y a renovar los contratos psicológicos con sus integrantes. La noción de confianza ha sido llevada del ámbito social al organizacional con la finalidad de lograr acuerdos entre organizaciones y al mismo tiempo lograr el compromiso de los integrantes de tales entidades. Para que estas puedan funcionar bajo este marco de transformaciones requieren de relaciones de colaboración regidas por la  confianza; relaciones que se dan en los niveles individual, dentro de la organización, entre organizaciones e institucionalmente. Los diferentes niveles de confianza están presentes en las relaciones que entablan los individuos, tanto dentro de la organización como en su entorno. Algunas de las reflexiones en torno a esta problemática nos llevan a pensar que el papel de la confianza es dinámico e incide en la transferencia y el intercambio de conocimientos, técnicas y procedimientos científicos, tecnologías, productos, capitales, entre otros. Además, las relaciones fundadas en la confianza requieren de la suspensión del propio interés, en beneficio de unaorientación colectiva (Mangematin y Thuderoz, 2003. Sin embargo, también se reconoce que con todo y la posibilidad que el hombre tiene de deliberar y de generar confianza, es evidente que entre los hombres prevalecen tanto la confianza calculada como los deseos de altruismo para entrar

  6. E. Coli and Pregnancy

    Science.gov (United States)

    ... best live chat Live Help Fact Sheets Share E. coli and Pregnancy Thursday, 20 November 2014 In ... pregnancy and while breastfeeding. Donate Sign Up For E-Newsletter Full Name * Email Address * Enter The Code: ...

  7. Highly selective and rapid arsenic removal by metabolically engineered Escherichia coli cells expressing Fucus vesiculosus metallothionein.

    Science.gov (United States)

    Singh, Shailendra; Mulchandani, Ashok; Chen, Wilfred

    2008-05-01

    An arsenic-chelating metallothionein (fMT) from the arsenic-tolerant marine alga Fucus vesiculosus was expressed in Escherichia coli, resulting in 30- and 26-fold-higher As(III) and As(V) binding, respectively. Coexpression of the As(III)-specific transporter GlpF with fMT further improved arsenic accumulation and offered high selectivity toward As. Resting E. coli cells coexpressing fMT and GlpF completely removed trace amounts (35 ppb) of As(III) within 20 min, providing a promising technology for compliance with the As limit of 10 ppb newly recommended by the U.S. EPA.

  8. Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein▿

    Science.gov (United States)

    Singh, Shailendra; Mulchandani, Ashok; Chen, Wilfred

    2008-01-01

    An arsenic-chelating metallothionein (fMT) from the arsenic-tolerant marine alga Fucus vesiculosus was expressed in Escherichia coli, resulting in 30- and 26-fold-higher As(III) and As(V) binding, respectively. Coexpression of the As(III)-specific transporter GlpF with fMT further improved arsenic accumulation and offered high selectivity toward As. Resting E. coli cells coexpressing fMT and GlpF completely removed trace amounts (35 ppb) of As(III) within 20 min, providing a promising technology for compliance with the As limit of 10 ppb newly recommended by the U.S. EPA. PMID:18326684

  9. Taxonomy Icon Data: Escherichia coli [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Escherichia coli Escherichia coli Escherichia_coli_L.png Escherichia_coli_NL.png Escherich...ia_coli_S.png Escherichia_coli_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Escherichia+co...li&t=L http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Escherichia+coli&t=NL http://biosciencedbc.jp/taxono...my_icon/icon.cgi?i=Escherichia+coli&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Escherichia+coli&t=NS ...

  10. El papel de las infraestructuras públicas en el desarrollo regional

    Directory of Open Access Journals (Sweden)

    Pablo Martín Urbano

    2005-01-01

    Full Text Available Dentro de las teorías del desarrollo endógeno, toma relevancia el papel que desempeñan las infraestructuras social y económica. Este papel no está exento de controversia y su impacto en el desarrollo de las regiones ha sido analizado desde varias perspectivas y experiencias. No obstante, en los últimos años se ha revalorado la influencia positiva de las infraestructuras, tanto, que su impacto en términos de externalidades puede ser tan importante como la inversión en capital humano o e desarrollo de tecnología. En este artículo se expone la teoría del desarrollo endógeno y se hace énfasis en el papel de la infraestructura, especialmente como pieza clave del potencial de desarrollo regional.

  11. O papel do biólogo no gerenciamento do perigo aviário em aeroportos = The role of biologist in airport bird hazard management

    Directory of Open Access Journals (Sweden)

    Bezerra, George Christian Linhares

    2011-01-01

    Full Text Available A segurança operacional da aviação demanda o gerenciamento do perigo aviário. A maior parte dos eventos de colisão entre aves e aeronaves ocorre nas proximidades dos aeroportos, o que está relacionado à menor altitude de voo e concentração de aves devido a fatores atrativos. O artigo foca o papel do profissional de ciências biológicas no processo de gerenciamento do perigo aviário em aeroportos, tendo como objetivo descrever as atividades por ele executadas. A pesquisa consistiu de revisão da literatura, identificação da regulamentação aplicável e as práticas adotadas e, através de estudo de caso realizado em aeroporto localizado na região amazônica, foi possível coletar informações de interesse para a pesquisa. Os resultados apontam que o biólogo atua especificamente na identificação do perigo, identificando focos de atração de aves, espécies presentes e hábitos de comportamento. Além disso, assessora a avaliação de risco com informações sobre a probabilidade da ocorrência de colisões e sobre a gravidade das suas consequências

  12. Quantitation of heparosan with heparin lyase III and spectrophotometry.

    Science.gov (United States)

    Huang, Haichan; Zhao, Yingying; Lv, Shencong; Zhong, Weihong; Zhang, Fuming; Linhardt, Robert J

    2014-02-15

    Heparosan is Escherichia coli K5 capsule polysaccharide, which is the key precursor for preparing bioengineered heparin. A rapid and effective quantitative method for detecting heparosan is important in the large-scale production of heparosan. Heparin lyase III (Hep III) effectively catalyzes the heparosan depolymerization, forming unsaturated disaccharides that are measurable using a spectrophotometer at 232 nm. We report a new method for the quantitative detection of heparosan with heparin lyase III and spectrophotometry that is safer and more specific than the traditional carbazole assay. In an optimized detection system, heparosan at a minimum concentration of 0.60 g/L in fermentation broth can be detected.

  13. La bioestadística y su papel en la investigación en salud

    OpenAIRE

    Rodríguez M., Nelcy

    2011-01-01

    La investigación en las áreas biológicas y muy especialmente en el área de la salud, es la base para la toma de decisiones que afectan a seres vivos. Este artículo hace una revisión del papel de la bioestadística como un área particular de la estadística en procesos de investigación, papel que resulta fundamental, dado que provee al investigador los elementos esenciales y suficientes para sustentar los resultados de sus observaciones y el análisis de las mismas.

  14. O papel de espelho em um Centro de Atenção Psicossocial

    OpenAIRE

    Franchini,Cristiane Bach; Campos,Elisa Maria Parahyba

    2008-01-01

    Este trabalho pretende refletir sobre o papel de espelho no tratamento oferecido em um centro de atenção psicossocial, o CAPS-T, utilizando-se o referencial psicanalítico com abordagem Winnicottiana. A mãe, que funciona como um espelho, reflete ao bebê, em seu olhar, algo que se refere a ele, e ser visto é uma das bases para o sentimento de existir, de forma que o "si-mesmo" verdadeiro, reconhecido, pode constituir-se. Quando o papel de espelho está ausente, o bebê não vê a si, o que pode lev...

  15. Business and human rights: el papel de los abogados de empresa

    OpenAIRE

    2014-01-01

    Los abogados de empresa juegan un papel fundamental para dotar de efectividad la aplicación de los principios Ruggie y el respeto de los Derechos Humanos en las empresas multinacionales. Están obligados a intervenir en la reparación de las violaciones de derechos actuando ante la Administración de Justicia, pero su papel es determinante en la prevención de los impactos sobre los derechos y en la evitación de los daños, actuando desde la responsabilidad ética y deontológica como conocedores de...

  16. The role of green rust in the environment: a review O papel do green rust no ambiente

    Directory of Open Access Journals (Sweden)

    Lucia H. G. Chaves

    2005-06-01

    Full Text Available Iron(II-iron(III hydroxy-salts known as green rusts (GRs initially were identified and studied as corrosion products of steel and iron. Recently they were discovered as minerals present in hydromorphic soils and sediments. Different studies have suggested that GRs, due to their high reactivity, play an important role in the fate and transport of many contaminants in suboxic soils, sediments and aquifers where microbial reduction rates are limited by low carbon content. Also, GRs have considerable potential for applications such as water-purification processes and gas adsorbents. However, as GRs are only a transient state during the reduction of ferric iron and are converted to more stable forms, it is still necessary to continue studies about their behavior.Hidróxidos de ferro (II-ferro(III conhecidos como "green rust" (GR, inicialmente foram identificados e estudados como sendo os produtos provenientes da corrosão do aço e do ferro. Recentemente esses hidróxidos foram encontrados na forma mineral em solos hidromórficos e em sedimentos. Devido a sua alta reatividade, muitos trabalhos têm mostrado que esses hidróxidos desempenham um importante papel no destino e transporte de muitos contaminantes em solos subóxicos, sedimentos e aqüíferos, onde a taxa de redução microbiana é limitada pelo baixo conteúdo de carbono. Os "green rusts" também podem ser utilizados nos processos de purificação de águas e como adsorventes de gases. Entretanto, como GR são considerados uma fase transitória durante a redução do íon férrico e são, rapidamente, convertidos para formas mais estáveis, é necessário que os estudos sobre seu comportamento continuem.

  17. Dietary N-Carbamylglutamate Supplementation Boosts Intestinal Mucosal Immunity in Escherichia coli Challenged Piglets.

    Science.gov (United States)

    Zhang, Fengrui; Zeng, Xiangfang; Yang, Fengjuan; Huang, Zhimin; Liu, Hong; Ma, Xi; Qiao, Shiyan

    2013-01-01

    N-carbamylglutamate (NCG) has been shown to enhance performance in neonatal piglets. However, few studies have demonstrated the effect of NCG on the intestinal mucosal barrier. This study was conducted to determine the effects of dietary NCG supplementation on intestinal mucosal immunity in neonatal piglets after an Escherichia coli (E. coli) challenge. New-born piglets (4 d old) were assigned randomly to one of four treatments (n = 7), including (I) sham challenge, (II) sham challenge +50 mg/kg NCG, (III) E. coli challenge, and (IV) E. coli challenge +50 mg/kg NCG. On d 8, pigs in the E. coli challenge groups (III and IV) were orally challenged with 5 mL of E. coli K88 (10(8) CFU/mL), whereas pigs in the sham challenge groups (I and II) were orally dosed with an equal volume of water. On d 13, all piglets were sacrificed, and samples were collected and examined. The results show that average daily gain in the E. coli challenged piglets (III and IV) was decreased (PE.coli<0.05). However, it tended to be higher in the NCG treated piglets (II and IV). Ileum secretory IgA, as well as IFN-γ, IL-2, IL-4 and IL-10 in ileal homogenates, were increased in E. coli challenged piglets (III and IV). Similarly, ileum SIgA and IL-10 levels, and CD4(+) percentage in NCG treated piglets (II and IV) were higher than no-NCG treated piglets (PNCG<0.05). However, the IL-2 level was only decreased in the piglets of E. coli challenge + NCG group (IV) compared with E. coli challenge group (III) (P<0.05). No change in the IL-2 level of the sham challenged piglets (III) was observed. In conclusion, dietary NCG supplementation has some beneficial effects on intestinal mucosal immunity in E. coli challenged piglets, which might be associated with stimulated lymphocyte proliferation and cytokine synthesis. Our findings have an important implication that NCG may be used to reduce diarrhea in neonatal piglets.

  18. Dietary N-Carbamylglutamate Supplementation Boosts Intestinal Mucosal Immunity in Escherichia coli Challenged Piglets.

    Directory of Open Access Journals (Sweden)

    Fengrui Zhang

    Full Text Available N-carbamylglutamate (NCG has been shown to enhance performance in neonatal piglets. However, few studies have demonstrated the effect of NCG on the intestinal mucosal barrier. This study was conducted to determine the effects of dietary NCG supplementation on intestinal mucosal immunity in neonatal piglets after an Escherichia coli (E. coli challenge. New-born piglets (4 d old were assigned randomly to one of four treatments (n = 7, including (I sham challenge, (II sham challenge +50 mg/kg NCG, (III E. coli challenge, and (IV E. coli challenge +50 mg/kg NCG. On d 8, pigs in the E. coli challenge groups (III and IV were orally challenged with 5 mL of E. coli K88 (10(8 CFU/mL, whereas pigs in the sham challenge groups (I and II were orally dosed with an equal volume of water. On d 13, all piglets were sacrificed, and samples were collected and examined. The results show that average daily gain in the E. coli challenged piglets (III and IV was decreased (PE.coli<0.05. However, it tended to be higher in the NCG treated piglets (II and IV. Ileum secretory IgA, as well as IFN-γ, IL-2, IL-4 and IL-10 in ileal homogenates, were increased in E. coli challenged piglets (III and IV. Similarly, ileum SIgA and IL-10 levels, and CD4(+ percentage in NCG treated piglets (II and IV were higher than no-NCG treated piglets (PNCG<0.05. However, the IL-2 level was only decreased in the piglets of E. coli challenge + NCG group (IV compared with E. coli challenge group (III (P<0.05. No change in the IL-2 level of the sham challenged piglets (III was observed. In conclusion, dietary NCG supplementation has some beneficial effects on intestinal mucosal immunity in E. coli challenged piglets, which might be associated with stimulated lymphocyte proliferation and cytokine synthesis. Our findings have an important implication that NCG may be used to reduce diarrhea in neonatal piglets.

  19. CyberStorm III

    NARCIS (Netherlands)

    Luiijf, H.A.M.; et al

    2010-01-01

    Projectteam Cyber Storm III - De Verenigde Staten organiseerden de afgelopen jaren een reeks grootschalige ICT-crisisoefeningen met de naam Cyber Storm. Cyber Storm III is de derde oefening in de reeks. Het scenario van Cyber Storm III staat in het teken van grootschalige ICT-verstoringen, waarbij n

  20. Global Positioning System III (GPS III)

    Science.gov (United States)

    2015-12-01

    Military Operations in Urban Terrain; Defense-Wide Mission Support; Air Mobility; and Space Launch Orbital Support. For military users, the GPS III...program provides Precise Positioning Service (PPS) to military operations and force enhancement. It also provides increased anti-jam power to the earth ...to be modified . On January 31, 2016, USD(AT&L) signed the GPS III revised APB. This Change 1 to the APB was due to both cost and schedule breaches

  1. Papel de los seguidores en el desarrollo de las teorías de liderazgo organizacional

    Directory of Open Access Journals (Sweden)

    Mónica García Solarte

    2015-06-01

    Full Text Available La mayoría de revisiones acerca del liderazgo se han enfocado en el líder y su papel dentro de la organización. Las investigaciones actuales demuestran que los seguidores, como un elemento importante del liderazgo, poseen un papel activo en la selección del estilo de liderazgo y en la teoría desde la cual se le evaluará a un líder. Desde este punto, el objetivo de este artículo es identificar cuál ha sido el papel de los seguidores en el desarrollo de las teorías del liderazgo organizacional. Al evaluar el papel que tiene el seguidor dentro del desarrollo de las teorías del liderazgo se pudo concluir que de acuerdo con su percepción, su comportamiento y sus necesidades, exige cierta categoría de líder, que puede ser vista desde cada teoría.

  2. taenia coli de cobaye

    African Journals Online (AJOL)

    AKA BOKO

    of the rhythmical contraction of guinea pig Taenia coli smooth muscle. ... déterminer le mécanisme d'action de ces composés. 2. Matériel et ..... [34, 35]. Il rappelle les effets de l'acétylcholine sur les muscles lisses viscéraux [36- 38]. Pour cela.

  3. Characterization of the biochemical properties of Campylobacter jejuni RNase III.

    Science.gov (United States)

    Haddad, Nabila; Saramago, Margarida; Matos, Rute G; Prévost, Hervé; Arraiano, Cecília M

    2013-11-25

    Campylobacter jejuni is a foodborne bacterial pathogen, which is now considered as a leading cause of human bacterial gastroenteritis. The information regarding ribonucleases in C. jejuni is very scarce but there are hints that they can be instrumental in virulence mechanisms. Namely, PNPase (polynucleotide phosphorylase) was shown to allow survival of C. jejuni in refrigerated conditions, to facilitate bacterial swimming, cell adhesion, colonization and invasion. In several microorganisms PNPase synthesis is auto-controlled in an RNase III (ribonuclease III)-dependent mechanism. Thereby, we have cloned, overexpressed, purified and characterized Cj-RNase III (C. jejuni RNase III). We have demonstrated that Cj-RNase III is able to complement an Escherichia coli rnc-deficient strain in 30S rRNA processing and PNPase regulation. Cj-RNase III was shown to be active in an unexpectedly large range of conditions, and Mn2+ seems to be its preferred co-factor, contrarily to what was described for other RNase III orthologues. The results lead us to speculate that Cj-RNase III may have an important role under a Mn2+-rich environment. Mutational analysis strengthened the function of some residues in the catalytic mechanism of action of RNase III, which was shown to be conserved.

  4. Enfermedad diarreica aguda por Escherichia coli patógenas en Colombia

    OpenAIRE

    Gómez-Duarte, Oscar G.

    2014-01-01

    Las cepas de E. coli patógenas intestinales son causas importantes de la enfermedad diarreica aguda (EDA) en niños menores de 5 años en América Latina, África y Asia y están asociadas a alta mortalidad en niños en las comunidades más pobres de África y el Sudeste Asiático. Estudios sobre el papel de las E. coli patógenas intestinales en la EDA infantil en Colombia y otros países de América Latina son limitados debido a la carencia de ensayos para detección de estos patógenos en los laboratori...

  5. Metallothionein (MT)-III

    DEFF Research Database (Denmark)

    Carrasco, J; Giralt, M; Molinero, A

    1999-01-01

    Metallothionein-III is a low molecular weight, heavy-metal binding protein expressed mainly in the central nervous system. First identified as a growth inhibitory factor (GIF) of rat cortical neurons in vitro, it has subsequently been shown to be a member of the metallothionein (MT) gene family...... and renamed as MT-III. In this study we have raised polyclonal antibodies in rabbits against recombinant rat MT-III (rMT-III). The sera obtained reacted specifically against recombinant zinc-and cadmium-saturated rMT-III, and did not cross-react with native rat MT-I and MT-II purified from the liver of zinc...... injected rats. The specificity of the antibody was also demonstrated in immunocytochemical studies by the elimination of the immunostaining by preincubation of the antibody with brain (but not liver) extracts, and by the results obtained in MT-III null mice. The antibody was used to characterize...

  6. Insect immune activation by recombinant Galleria mellonella apolipophorin III(1).

    Science.gov (United States)

    Niere, M; Meisslitzer, C; Dettloff, M; Weise, C; Ziegler, M; Wiesner, A

    1999-08-17

    Apolipophorin III (apoLp-III) is an exchangeable insect apolipoprotein. Its function, as currently understood, lies in the stabilization of low-density lipophorin particles (LDLp) crossing the hemocoel in phases of high energy consumption to deliver lipids from the fat body to the flight muscle cells. Recent studies with native Galleria mellonella-apoLp-III gave first indications of an unexpected role of that protein in insect immune activation. Here we report the immune activation by the recombinant protein, documenting a newly discovered correlation between lipid physiology and immune defense in insects. The complete cDNA sequence of G. mellonella-apoLp-III was identified by mixed oligonucleotide-primed amplification of cDNA (MOPAC), 3'-RACE-PCR, and cRACE-PCR. The sequence coding for the native protein was ligated into a pET-vector; this construct was transfected into Escherichia coli and overexpressed in the bacteria. Photometric turbidity assays with human low density lipoprotein (LDL) and transmission electron microscopy studies on apoLp-III-stabilized lipid discs revealed the full functionality of the isolated recombinant apoLp-III with regard to its lipid-association ability. For proving its immune-stimulating capacity, apoLp-III was injected into the hemocoel of last instar G. mellonella larvae and the antibacterial activity in cell-free hemolymph was determined 24 h later. As a result, the hemolymph samples of injected insects contained strongly increased antibacterial activities against E. coli as well as clearly enhanced lysozyme-like activities. From Northern blot analysis of total RNA from insects injected with apoLp-III or the bacterial immune provocator lipopolysaccharide, it could be concluded that the transcription rate of apoLp-III mRNA does not vary in comparison to untreated last instar larvae.

  7. El papel de algunas instituciones sociales en el derecho a decidir sobre la regulación de la fecundidad

    OpenAIRE

    Ivonne Szasz

    2002-01-01

    El artículo presenta algunas posiciones que se debaten en la bibliografía actual sobre el papel de las instituciones sociales en la toma de decisiones sobre los eventos reproductivos y su regulación. Se plantean algunos términos del debate sobre los derechos reproductivos y el papel de las instituciones públicas de salud, distinguiendo entre el desempeño de esas instituciones en distintos tipos de escenarios estatales. Se analiza también el papel que pueden desem...

  8. Enfermedad diarreica aguda por Escherichia coli patógenas en Colombia

    Science.gov (United States)

    Gómez-Duarte, Oscar G.

    2014-01-01

    Resumen Las cepas de E. coli patógenas intestinales son causas importantes de la enfermedad diarreica aguda (EDA) en niños menores de 5 años en América Latina, África y Asia y están asociadas a alta mortalidad en niños en las comunidades más pobres de África y el Sudeste Asiático. Estudios sobre el papel de las E. coli patógenas intestinales en la EDA infantil en Colombia y otros países de América Latina son limitados debido a la carencia de ensayos para detección de estos patógenos en los laboratorios clínicos de centros de salud. Estudios recientes han reportado la detección de E. coli patógenas intestinales en Colombia, siendo la E. coli enterotoxigénica la cepa más frecuentemente asociada a diarrea en niños menores de 5 años. Otros patógenos detectados en estos pacientes incluyen las E. coli enteroagregativa, enteropatógena, productora de toxina Shiga, y de adherencia difusa. Con base en estudios que reportan la presencia de E. coli productora de toxina Shiga y E. coli enteroagregativa en carnes y vegetales en supermercados, se cree que productos alimentarios contaminados contribuyen a la transmisión de estos patógenos y a la infección del huésped susceptible. Más estudios son necesarios para evaluar los mecanismos de transmisión, el impacto en la epidemiologia de la EDA, y las pautas de manejo y prevención de estos patógenos que afectan la población pediátrica en Colombia. PMID:25491457

  9. PART I. ESCHERICHIA COLI

    Directory of Open Access Journals (Sweden)

    Sanaa Mahdi Oraibi

    2016-11-01

    Full Text Available The presence of Escherichia coli in the air of facilities involved in management and composting of post-slaughter poultry wastes in selected plants of West Western Pomerania region was studied. Measurements were made on four dates in a variety of weather conditions during the year. The study was conducted at 5 objects that differ in the type of waste and the degree of preparation for composting. These were: chemical treatment and preliminary processing plant, liquid wastes reservoir, platform for preparation of materials for composting, storage of biological sediments, and composting facility. Measurement of bacteria count was carried out in accordance with the applicable procedures on selective chromogenic TBX medium. The assays revealed the presence of E. coli at all test objects, but not always on all measurement dates. It has been shown that the presence of E. coli was from 20 to 3047 CFU∙m-3 of air, although the largest quantities were most frequently detected in the air of the building for post-slaughter waste pre-treatment in chemical treatment plant.

  10. Aplicabilidade da CIPE® fundamentada na teoria da modelagem e modelagem de papel

    Directory of Open Access Journals (Sweden)

    Manuela de Mendonça Figueirêdo Coelho

    2014-06-01

    Full Text Available Objetivou-se analisar a proposta de plano educativo organizado por meio do processo de enfermagem, utilizando a Classificação Internacional para a Prática de Enfermagem (CIPE®, fundamentada pela Teoria da Modelagem e Modelagem de Papel, a um grupo de mulheres em Maracanaú-CE, Brasil. Trata-se de um estudo de caso realizado em grupo educativo de mulheres. A abordagem qualitativa conduziu a produção dos dados guiados pelo Método Criativo e Sensível. Dois diagnósticos centrais emergiram das necessidades apresentadas pelo grupo: saúde comprometida e conhecimento adequado, assim como formulação de resultados e intervenções de enfermagem. O estudo aponta a possibilidade de aplicação do processo de enfermagem apoiado pela CIPE® e ações educativas baseadas na Teoria da Modelagem e Modelagem de Papel.

  11. O papel da ilusão na psicanálise Freudiana

    Directory of Open Access Journals (Sweden)

    Zeferino Rocha

    2012-12-01

    Full Text Available Pretende-se descrever o papel atribuído por Freud à Ilusão nas três grandes fases da elaboração da teoria psicanalítica, tentando mostrar como a ilusão, quando confrontada com a prova da realidade, pode se tornar uma fonte de criatividade com um papel decisivo na construção da subjetividade.The role of illusion in the Freudian psychoanalysis. The article has as purpose to describe the role assigned by Freud to the Illusion, in the elaboration phases of the psychoanalytic theory, trying to show the illusion, when faced with the reality's proof, can become a source of criativity with a decisive role in the subjectivity construction.

  12. El papel de la banca pública en las crisis financieras de Argentina y Uruguay

    Directory of Open Access Journals (Sweden)

    Wesley Marshall

    2013-01-01

    Full Text Available El objetivo de este artículo es subrayar la naturaleza contracíclica de la banca pública comercial. Esto se hará mediante un análisis histórico de las crisis bancarias en Argentina y Uruguay a principios del siglo xxi. Este tipo de banco abarcó en Argentina varios actores cruciales dentro de las exitosas políticas de resolución de crisis y de reestructuración financiera; mientras en Uruguay, la banca pública comercial tuvo un papel menor durante la fase de resolución de la crisis, pero jugó un papel fundamental en la minimización de los riesgos del sistema financiero que en parte provocaron la crisis de 2002.

  13. Intinerario de una Salida Negociada y El Papel de la Sociedad Civil en el Caso Centroameriano

    Directory of Open Access Journals (Sweden)

    Rosembert Ariza Santamaría

    1999-01-01

    Full Text Available La sociedad civil jugó un papel preponderante en el proceso Centroamericano; Nicaragua, el Salvador y Guatemala contaron con el concurso de sus ciudadanos en el propósito de la paz, en cada país su papel fue distinto, pero su fin era el mismo. Los tres procesos de paz nos dejan lecciones que podemos retomar como referente en las negociaciones de paz que va a iniciar el gobierno Colombiano. La creación de la Asamblea de la Sociedad Civil (A.S.C. el "Intergremial" en el Salvador y el voto del pueblo Nicaragüense fueron expresiones de una sociedad que ante el conflicto armado no posó de indiferente, sino por el contrario se aferró a la búsqueda de una solución negociada en lugar de una victoria militar.

  14. Papel da enfermagem na reabilitação física

    Directory of Open Access Journals (Sweden)

    Leonardo Tadeu de Andrade

    2010-12-01

    Full Text Available Este estudo revisa a literatura sobre o desenvolvimento do papel do enfermeiro na reabilitação e sugere formas dos enfermeiros alcançarem as suas potencialidades nessa área. Algumas questões foram levantadas para provocar reflexão e discussão. É evidente, a partir da literatura, que os enfermeiros têm um papel integral na reabilitação, mas estes limites não são claramente definidos. Os enfermeiros precisam aliar a prática em reabilitação com a educação e formação, o que contribuirá para uma maior eficácia e influência no processo e nos serviços de reabilitação.

  15. Extracto acuoso de Lepidium meyenii Walp (maca y su papel como adaptógeno, en un modelo animal de resistencia física

    Directory of Open Access Journals (Sweden)

    Silvia Suárez

    2009-09-01

    Full Text Available Introducción: El Lepidium meyenii Walp (maca es una raíz andina del Perú, utilizada como alimento por su valor nutricional y propiedades etnomedicinales; es parte de la medicina tradicional. Objetivos: Evaluar el papel de adaptógeno del extracto acuoso de maca amarilla sobre las enzimas del tejido muscular en un modelo animal de resistencia física y estrés oxidativo. Diseño: Experimental. Institución: Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, UNMSM, Lima, Perú. Material biológico: Extracto acuoso de la maca amarilla y ratas macho recién destetados. Intervenciones: Administración del extracto acuoso de la maca amarilla en ratas macho recién destetados, distribuidos en cuatro grupos: (I, de control, (II 0,4 mg de maca/g de peso (III 0,8 mg de maca/g de peso y (IV, 1,2 mg de maca/g de peso. El extracto acuoso fue administrado por cánula orogástrica. Se realizó un control de los pesos. Se aplicó la prueba de nado forzado después de 30 días de tratamiento. Los animales fueron sacrificados y se preparó homogenizado de músculo al 10%. Principales medidas de resultados: Actividad de las enzimas superóxido dismutasa (SOD, catalasa (CAT y lactato deshidrogenasa (LDH; y, como indicador de proceso oxidativo, se midió la peroxidación lipídica (TBARS. Resultados: El rendimiento en la prueba de resistencia fue la siguiente: (I 7,09 min; (II 11,25 min; (III 11,27 min; (IV 12,71 min, respectivamente. Las actividades enzimáticas de los grupos I, II, III y IV fueron: SOD (U/mL 36,6, 18,2, 17,2 y 18,2; CAT (U/L 18,6, 16,5, 13,4 y 10,3; y LDH (U/mL 11,6, 6,5, 6,0 y 5,8. TBARS (umol/g tejido: 5,82, 7,15, 4,11 y 4,06. Conclusiones: La administración del extracto acuoso de maca amarilla favorece la respuesta del organismo a una situación estresante y físicamente extenuante, lo que correspondería al papel de un adaptógeno.

  16. La desrusificación de Ucrania. El papel de los medios

    Directory of Open Access Journals (Sweden)

    Antonio Checa Godoy

    2008-01-01

    Full Text Available La presencia de una importante minoría de habla rusa en el seno de Ucrania (22%, plantea numerosos problemas en el proceso de normalización del idioma ucraniano. Los medios tienen un papel decisivo en ese proceso; tras casi dos décadas de independencia, el ruso mantiene posiciones dominantes o claves en prácticamente todos los sectores de la comunicación.

  17. Papel de los anticuerpos en la protección contra micobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Nesty Olivares

    2006-12-01

    Full Text Available Convencionalmente se asume que la defensa del hospedero contra Mycobacterium tuberculosis se basa en los mecanismos de inmunidad celular exclusivamente y se descarta el papel de los anticuerpos en la protección. En este trabajo se analizan evidencias recientes que retan este dogma y sugieren la importancia de considerar la manipulación de la respuesta inmune humoral como una alternativa en la investigación de vacunas contra la tuberculosis.

  18. El papel de la enfermera en el Síndrome de Down

    OpenAIRE

    Tirador Barroso, Nuria

    2015-01-01

    El síndrome de Down es la principal causa de discapacidad intelectual por ello, este trabajo realizado mediante una revisión bibliográfica de protocolos y diversos artículos científicos determinar cual es el papel de la enfermera en este síndrome y en qué áreas la enfermera puede actuar para buscar la mejor calidad de vida en estas personas.

  19. La retribución variable de los directivos y el papel de las stock options

    OpenAIRE

    García Echevarría, Santiago

    2000-01-01

    En la segunda mitad del siglo XX se ha producido un cambio acelerado de la politica retributiva, no sólo de los directivos sino de todo personal de las empresas. Se está imponiendo la cultura de la retribución variable, que conlleva una relación directa entre las retribuciones y las prestaciones. Se analiza el porqué de este cambio, así como el papel que las "stock options" juegan en el mismo.

  20. Predictores familiares de la violencia filio-parental: el papel de la disciplina familiar

    OpenAIRE

    Izaskun Ibabe

    2015-01-01

    La violencia filio-parental es un problema social cualitativamente diferente a otros tipos de violencia familiar, porque los adolescentes dirigen la violencia hacia quienes debieran representar la autoridad y proporcionarles bienestar. El objetivo principal de este estudio era analizar el papel de las relaciones paternofiliales y la disciplina familiar en el desarrollo de conductas violentas y prosociales de los adolescentes hacia sus padres. En el estudio participaron 585 hijos/as (48% varon...

  1. Filigranas y contramarcas de papel en los documentos de la Junta de Temporalidades (1767-1785

    Directory of Open Access Journals (Sweden)

    De Loredo, Carolina

    2010-01-01

    Full Text Available Este trabajo tiene por objeto el estudio y análisis diplomático de las filigranas o marcas de agua y contramarcas de papel, teniendo en cuenta el valor diplomático que presentan las mismas. Estas se hallan impresas en las hojas de papel encontradas en los documentos de la Junta de Temporalidades de Córdoba redactados entre los años de 1767 y 1785. Nuestro interés radica no sólo por ser éstas uno de los componentes externos que hacen al soporte físico de la escritura, sino que constituyen además uno de los rasgos cruciales para determinar el principio de procedencia y autenticidad documental, siendo estos uno de los objetos principales de análisis de la Diplomática. Utilizaremos como base de este trabajo las filigranas papeleras que se encuentran en la documentación de Temporalidades del A.H.P.C, A.G.H.U.N.C y Fondo Documental Mons. Pablo Cabrera (ex Instituto Americanista con el fin de identificarlas según sus motivos, formas, dibujos y figuras; y poder investigar a través de ellas, qué procedencia tenía el papel que circulaba en esta parte de las Indias durante el siglo XVIII.

  2. Hidrotransporte de pulpa de papel en un canal abierto de sección circular

    Directory of Open Access Journals (Sweden)

    Hernán Javier Gómez Zambrano

    2009-01-01

    Full Text Available Este artículo presenta los resultados de la investigación experimental llevada a cabo sobre el transporte hidráulico de mezclas en canales abiertos. Se investigó acerca de las mezclas no sedimentables compuestas de pulpa de papel y agua en un canal abierto de sección transversal circular. El objeto de la investigación fue determinar las pérdidas de energía debido al flujo de agua y pulpa de papel, a fin de proponer una metodología para el diseño de canales abiertos a mezclas. A causa del desarrollo experimental se construyó un montaje de laboratorio en el cual se adaptó un canal circular con pendiente variable. Como resultado se lograron obtener nuevas ecuaciones para el coeficiente de fricción de Darcy- Weisbach y el coeficiente de fricción de Manning, que relacionan estos coeficientes con las propiedades del agua, con las de la mezcla y la pendiente del canal. Con las ecuaciones obtenidas en la investigación, el autor propuso una nueva metodología para el diseño de canales que transporten mezclas de agua y pulpa de papel.

  3. Synthesis and Structural Studies of Cr(III, Mn(II and Fe(III Complexes of N(2-Benzimidazolylacetylacetohydrazone

    Directory of Open Access Journals (Sweden)

    G. H. Anuradha

    2011-01-01

    Full Text Available The ligand N(2-benzimidazolylacetylacetohydrazone (BAAH have been synthesized and characterized. Coordination complexes of Cr(III, Mn(II and Fe(III have been synthesized with the ligand BAAH. These complexes were characterized on the basis of analytical, conductance, thermal, magnetic data and infrared and electronic spectral data. The ligand BAAH is behaving as a neutral tridentate NNO donar employing two azomethine nitrogens (ring and side chain and carbonyl oxygen. The ligand and it's metal complexes were tested for anti microbial activity on the gram positive S. Aureus, E. coli and Proteus.

  4. The type III manufactory

    CERN Document Server

    Palcoux, Sébastien

    2011-01-01

    Using unusual objects in the theory of von Neumann algebra, as the chinese game Go or the Conway game of life (generalized on finitely presented groups), we are able to build, by hands, many type III factors.

  5. A possible role for chromium(III) in genotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Snow, E.T. (New York Univ. Medical Center, Tuxedo (United States))

    1991-05-01

    Chromium is found in the environment in two major forms: reduced Cr{sup III} and Cr{sup VI}, or chromate. Chromate, the most biologically active species, is readily taken up by living cells and reduced intracellularly, via reactive intermediates, to stable Cr{sup III} species. Cr{sup III}, the most abundant form of chromium in the environment, does not readily cross cell membranes and is relatively inactive in vivo. However, intracellular Cr{sup III} can react slowly with both nucleic acids and proteins and can be genotoxic. The authors have investigated the genotoxicity of Cr{sup III} in vitro using a DNA replication assay and in vivo by CaCl{sub 2}-mediated transfection of chromium-treated DNA into Escherichia coli. These results suggest that Cr{sup III} alters the interaction between the DNA template and the polymerase such that the binding strength of the DNA polymerase is increased and the fidelity of DNA replication is decreased. These interactions may contribute to the mutagenicity of chromium ions in vivo and suggest that Cr{sup III} can contribute to chromium-mediated carcinogenesis.

  6. Asymptomatic bacteriuria Escherichia coli strains

    DEFF Research Database (Denmark)

    Hancock, Viktoria; Nielsen, E.M.; Klemm, Per

    2006-01-01

    Urinary tract infections (UTIs) affect millions of people each year. Escherichia coli is the most common organism associated with asymptomatic bacteriuria (ABU) in humans. Persons affected by ABU may carry a particular E. coli strain for extended periods of time without any symptoms. In contrast...

  7. Asymptomatic bacteriuria Escherichia coli strains

    DEFF Research Database (Denmark)

    Hancock, Viktoria; Nielsen, E.M.; Klemm, Per

    2006-01-01

    Urinary tract infections (UTIs) affect millions of people each year. Escherichia coli is the most common organism associated with asymptomatic bacteriuria (ABU) in humans. Persons affected by ABU may carry a particular E. coli strain for extended periods of time without any symptoms. In contrast...

  8. Occurrence of adhesin-encoding operons in Escherichia coli isolated from breeders with salpingitis and chicks with omphalitis Ocorrência de operons codificadores de adesinas em Escherichia coli isolada de aves reprodutoras com salpingite e de pintinhos com onfalite

    Directory of Open Access Journals (Sweden)

    Terezinha Knöbl

    2006-06-01

    Full Text Available The occurrence of fim, pap and sfa operons in Escherichia coli isolated from breeders with salpingitis and chicks with omphalitis was evaluated. Analysis of 100 E. coli isolates, by colony hybridization tests, showed that 78 (78% were fim+, one (1% was sfa+, seven (7% were fim+ associated with pap+, eigth (8% were fim+ and sfa+, one (1% was fim+pap+sfa+ and five (5% isolates did not hybridize with any probe. These results suggest that fim adhesion-encoding operon plays an important role in pathogenesis of E. coli infection in chickens with salpingitis and omphalitis.Ocorrência dos operons fim, pap e sfa em amostras de Escherichia coli isoladas de reprodutoras com salpingite e pintinhos com onfalite foi avaliada. A análise de 100 amostras através dos testes de hibridização de colônia mostrou que 78 (78% amostras eram fim+, uma (1% era sfa+, sete (7% eram fim+ associada a pap+, oito (8% eram fim+ e uma (1% era fim+pap+sfa+ e cinco (5% amostras não hibridizaram com nenhuma sonda. Estes resultados sugerem que o operon fim pode ter um importante papel na patogenia da infecção de Escherichia coli em reprodutoras com salpingite e pintinhos com onfalite.

  9. Caracterização molecular, formação de biofilme e susceptibilidade a antimicrobianos de isolados de E. coli de aderência difusa Afa/Dr e não Afa/Dr

    OpenAIRE

    Fagundes, Laura Kreuz

    2013-01-01

    A Escherichia coli de aderência difusa (DAEC), um patotipo diarreiogênico de E. coli, corresponde a um grupo heterogêneo sem marcador de virulência comum a todos os isolados e de papel controverso na diarreia infantil. O objetivo deste estudo foi caracterizar genotipica e fenotipicamente amostras de DAEC, portadoras e não portadoras de adesinas Afa/Dr, isoladas de crianças com e sem diarreia. Em 70 amostras de DAEC, PCR foi realizado para pesquisa de genes descritos em DAEC, EAEC ou UPEC, que...

  10. O Papel da Psicoterapia de Grupo na Formação do Residente em Psiquiatria

    Directory of Open Access Journals (Sweden)

    Cláudia de Paula Juliano Souza

    Full Text Available RESUMO O objetivo deste trabalho foi analisar o papel da psicoterapia de grupo na formação do residente em psiquiatria do Programa de Residência Médica em Psiquiatria da Universidade Federal de Goiás. Trata-se de um estudo descritivo exploratório com abordagem qualitativa em educação médica. Os dados foram coletados por meio de relatórios descritivos e entrevistas semiestruturadas, submetidas à análise de conteúdo temático-categorial. Emergiram da análise dos dados duas categorias: as ações educativas do ensino da psicoterapia de grupo e as ações sociais do ensino da psicoterapia de grupo. Na análise da primeira categoria, obtivemos cinco subcategorias: relação médico-paciente, aprendizagem cognitiva, aprendizagem afetiva, diálogo interdisciplinar e desenvolvimento pessoal. Na segunda categoria, obtivemos duas subcategorias: socialização e encontro. Conclui-se que o ensino da psicoterapia de grupo tem papel educativo, pois contribui com a inovação dos cenários de prática, possibilitando mudanças na relação médico residente-paciente e consolidando o conceito ampliado de saúde na perspectiva da integralidade. Revela também seu papel social, pois contribui para uma aproximação sociointerativa entre preceptor-residente-grupo.

  11. Tir Is Essential for the Recruitment of Tks5 to Enteropathogenic Escherichia coli Pedestals

    DEFF Research Database (Denmark)

    Jensen, Helene Halkjær; Pedersen, Hans Nymand; Stenkjær, Eva;

    2015-01-01

    Abstract Enteropathogenic Escherichia coli (EPEC) is a bacterial pathogen that infects the epithelial lining of the small intestine and causes diarrhea. Upon attachment to the intestinal epithe- lium, EPEC uses a Type III Secretion System to inject its own high affinity receptor Translo- cated...

  12. Recombinant lipidated dengue-3 envelope protein domain III stimulates broad immune responses in mice.

    Science.gov (United States)

    Chiang, Chen-Yi; Liu, Shih-Jen; Hsieh, Chun-Hsiang; Chen, Mei-Yu; Tsai, Jy-Ping; Liu, Hsueh-Hung; Chen, I-Hua; Chong, Pele; Leng, Chih-Hsiang; Chen, Hsin-Wei

    2016-02-17

    The linkage of an immunogen with a toll-like receptor ligand has great potential to induce highly potent immune responses with the initial features of antigen-presenting cell activation. In the current study, we expressed recombinant dengue-3 envelope protein domain III (D3ED III) in lipidated form using an Escherichia coli-based system. The recombinant lipidated dengue-3 envelope protein domain III (LD3ED III) augments the expression levels of IL-12 family cytokines. LD3ED III-immunized mice enhance wide ranges of T cell responses as indicated by IFN-γ, IL-17, IL-21 production. Additionally, LD3ED III-immunized mice increase the frequencies of anti-D3ED III antibody producing cells. The boosted antibody titers cover various IgG isotypes, including IgG1, IgG2a, IgG2b, and IgG3. Importantly, LD3ED III-immunized mice induce neutralizing antibody capacity associated with a reduction of viremia levels after challenges. In contrast, mice that are immunized with D3ED III formulated with aluminum phosphate (D3ED III/Alum) only enhance Th2 responses and boost IgG1 antibody titers. Neither neutralizing antibody responses nor the inhibition of viremia levels after challenge is observed in mice that are immunized with D3ED III/Alum. These results suggest that LD3ED III can induce broad profiles of cellular and humoral immune responses.

  13. A curva da demanda e seu papel na institucionalização do marketing

    OpenAIRE

    Morici, Riccardo Vanni

    2014-01-01

    O Objetivo dessa dissertação é compreender o papel que as Ciências Econômicas, e, especialmente a Curva da Demanda popularizada pelo economista britânico Alfred Marshall, tiveram no processo de institucionalização da área de Marketing. Marshall foi um dos responsáveis pela institucionalização das Ciências Econômicas no final do século XIX. Pretendemos avaliar se a Curva da Demanda, um dos elementos trabalhados por Marshall em seu livro principal Principles of Economics vol. I (1890) estev...

  14. O papel das plataformas de streaming na distribuição de filmes independentes

    OpenAIRE

    Batista, André Ramos Santos Sarmento

    2016-01-01

    A presente dissertação tem como objetivo analisar o papel das plataformas de streaming na distribuição de filmes independentes. Pretende-se investigar as transformações ocorridas nos processos de disseminação destas obras no contexto da sociedade em rede e também conhecer os diferentes motivos que levam os cineastas responsáveis pelas mesmas a adotar as plataformas de streaming como meios de difusão para o seu trabalho. A hipótese que se coloca é a de que estes serviços são uti...

  15. Dirección de arte enfocado al rediseño de club de papel

    OpenAIRE

    Marrasquin Briones, Karla Pamela; Baque Galindo, Martha Vanessa; Peña Zapatier, Gabriel Isidro

    2013-01-01

    El proyecto que llevamos a cabo se basa en el rediseño de la línea gráfica del programa piloto Club de Papel destinado para Espol Tv. Debido a la falta de conceptos visuales hemos trabajado para equilibrar el contenido gráfico de la imagen del programa a realizarse, mejorando su contenido, caracterización y aceptación por parte de los televidentes. El cambio de imagen radica sobre la dirección de arte en dicha producción, teniendo como objetivo el manejo adecuado sobre la nueva propuesta g...

  16. O papel da atenção na fisioterapia neurofuncional

    OpenAIRE

    Andréa Lúcia Sério Bertoldi; Vera Lúcia Israel; Iverson Ladewig

    2011-01-01

    Este estudo apresenta uma revisão teórica sobre o papel da atenção na realização de tarefas motoras, adotando-se a perspectiva do paradigma sistêmico aplicado ao comportamento motor e sua relação com a abordagem do controle motor na fisioterapia neurofuncional. Foram consultados os bancos de dados Medline, PubMed, LILACS, SciELO, e PEDro, incluindo artigos de 1990 a 2009. Nos estudos pesquisados, observou-se o entendimento da atenção como um agente mediador entre o indivíduo e o ambiente no p...

  17. Papel del estrés en la intensidad de los síntomas SII

    OpenAIRE

    Pletikosic, Sanda; TKALČIĆ, Mladenka

    2016-01-01

    Síndrome del intestino irritable se considera un trastorno biopsicosocial, el resultado de la combinación compleja de factores predisponentes, precipitantes y perpetuos. Rasgos de personalidad, estado afectivo y estrés son algunos de los factores relevantes que contribuyen a la calidad de vida más baja y la exacerbación sintomática de los pacientes con SII. Para examinar el papel del estrés en la exacerbación sintomática de SII, el objetivo de este estudio era explorar la relación entre los a...

  18. Sustentabilidad del sistema de papel amate en San Pablito, Pahuatlán, Puebla.

    OpenAIRE

    Fuentes Castillo, María Janet

    2014-01-01

    La investigación expone el nivel de sustentabilidad en que se encuentra el sistema de producción de papel amate en San Pablito, Pahuatlán, Puebla; se fundamenta en la teoría del desarrollo sustentable. La metodología utilizada en la investigación es mixta (cuantitativa y cualitativa). Los datos se obtuvieron a través de un cuestionario aplicado a 76 familias artesanas; se utilizaron técnicas de observación y observación participante; además el Marco para la Evaluación de Sistemas de Manejo de...

  19. El papel de las ciudades en la configuración del reino de Valencia

    OpenAIRE

    Domingo Pérez, Concepción; Teixidor de Otto, María Jesús; Barceló, Carmen

    1984-01-01

    En la organización del territorio valenciano la conquista cristiana por Jaime I viene a representar un hito decisivo en el posterior evolución del poblamiento. La colonización del recién creado reino significó una ruptura evidente con la época anterior, una de cuyas facetas básicas fue el interés por desarrollar asentamientos "urbanos", como centros de poder político, religioso y económico, que ejercieron un papel articulador y que sientan las bases de su futura red de ciudades.

  20. O papel da atenção na fisioterapia neurofuncional

    OpenAIRE

    2011-01-01

    Este estudo apresenta uma revisão teórica sobre o papel da atenção na realização de tarefas motoras, adotando-se a perspectiva do paradigma sistêmico aplicado ao comportamento motor e sua relação com a abordagem do controle motor na fisioterapia neurofuncional. Foram consultados os bancos de dados Medline, PubMed, LILACS, SciELO, e PEDro, incluindo artigos de 1990 a 2009. Nos estudos pesquisados, observou-se o entendimento da atenção como um agente mediador entre o indivíduo e o ambiente no p...

  1. El papel de la vestimenta en los rituales mexicas de “personificación”

    OpenAIRE

    Dehouve, Danièle

    2016-01-01

    Los mexicas o aztecas del centro de México utilizaban en sus ceremonias ciertas prendas de vestir tejidas de algodón o de fibras vegetales, o simplemente hechas de papel de amate, y cubiertas de diseños. El presente artículo procura explicar la variedad de dicha indumentaria ritual con la ayuda del concepto nativo de “personificación” (ixiptla, en náhuatl), en función del cual se cubría un soporte (el “personificador”) con una indumentaria y varios adornos pertenecientes a un dios (el “person...

  2. Estudio del papel fisiopatológico de la resistencia a la insulina en la cirrosis

    OpenAIRE

    Erice Muñoz, Eva

    2014-01-01

    Los factores de riesgo cardiovascular, como la hipertensión arterial, la diabetes o la dislipemia, están íntimamente relacionados con el fenómeno de resistencia a la insulina, mecanismo fisiopatológico responsable de la aparición de disfunción endotelial y del desarrollo de complicaciones cardiovasculares. Este fenómeno también se encuentra en la cirrosis hepática. Su presencia se ha relacionado con el desarrollo de diabetes mellitus. Sin embargo, se desconoce si juega un papel en la disf...

  3. Estudio del papel fisiopatológico de la resistencia a la insulina en la cirrosis

    OpenAIRE

    Erice Muñoz, Eva

    2015-01-01

    Los factores de riesgo cardiovascular, como la hipertensión arterial, la diabetes o la dislipemia, están íntimamente relacionados con el fenómeno de resistencia a la insulina, mecanismo fisiopatológico responsable de la aparición de disfunción endotelial y del desarrollo de complicaciones cardiovasculares. Este fenómeno también se encuentra en la cirrosis hepática. Su presencia se ha relacionado con el desarrollo de diabetes mellitus. Sin embargo, se desconoce si juega un papel en la disfunci...

  4. El papel del ingeniero mecánico en el contexto energético actual

    OpenAIRE

    María Dolores Durán García

    2010-01-01

    En el presente trabajo se analiza, en primer término, la evolución del papel del ingeniero a través de la historia, desde sus orígenes en la Revolución Industrial hasta nuestros días; asimismo se abordan las diferentes formas en que se ha ido concibiendo la función del ingeniero mecánico y los efectos de la situación económica, tecnológica y medioambiental en estas percepciones sociales. Esta última implicación, la ambiental, sirve de base para establecer la responsabilidad actual de este pro...

  5. O papel da gestão de marcas para as PMEs

    OpenAIRE

    Bento, Sandrine da Silva

    2014-01-01

    Dissertação de mestrado, Marketing, Faculdade de Economia, Universidade do Algarve, 2014 Sendo a mestranda gestora de projeto (e sócia-gerente) de uma agência de design de comunicação (Newings design agency.), depara-se com a necessidade no seu dia-a-dia profissional, de informar a importância do marketing e, principalmente, da gestão de marcas. O desafio deste relatório de atividade profissional passa por entender o papel da gestão de marcas nas PMEs, e qual a sua proximid...

  6. Papel de las sirtuinas en el envejecimiento de cèlulas madre adultas

    OpenAIRE

    García García, María

    2013-01-01

    Las Sirtuinas son una familia de desacetilasas dependientes de NAD que juegan un papel fundamental en un gran número de procesos biológicos como la regulación del estado energético de la célula, la diferenciación celular y el envejecimiento. En mamíferos, los 7 miembros de la familia conocidos (Sirt 1-7) son capaces de desacetilar proteínas como TP53, FOXOs o histonas. El primer miembro de la familia, Sirtuina 1, fue inicialmente identificado en levadura (Sir2) como una proteína impo...

  7. El papel de la memoria en el aprendizaje de una segunda lengua

    OpenAIRE

    Rodríguez-Méndez, Silvia

    2013-01-01

    Aprendizaje y memoria son dos procesos con una relación muy estrecha, más si cabe dentro del campo de los idiomas. La comprensión de una segunda lengua resultaría imposible sin un uso eficiente de nuestra capacidad de memoria. Por ello, establecer el papel concreto de este proceso en la adquisición de una lengua extranjera supone aportar nuevas vías para la mejora de este aprendizaje. Así, el objetivo del presente estudio ha sido analizar el rol ejercido por los diferentes mecanismos de la me...

  8. Papel de la enfermería en las hemorragias digestivas altas en urgencias y emergencias

    OpenAIRE

    Ibáñez García, Patricia

    2015-01-01

    La hemorragia digestiva alta es la complicación gastroenterológica más importante. Está delimitada por el esfínter esofágico superior y el ángulo de Treitz, es decir, es aquella que se produce en esófago, estómago o duodeno. El objetivo de esta revisión bibliográfica es conocer cuál es el papel que ejerce la enfermería ante una hemorragia digestiva alta en las emergencias extrahospitalarias y urgencias hospitalarias. Se diferencian dos tipos de hemorragias según la causa que las produzc...

  9. Papel de las apolipoproteinas del fluido cerebroespinal embrionario como factores reguladores de la supervivencia celular

    OpenAIRE

    Carretero Soto, Raquel

    2013-01-01

    El Fluido Cerebroespinal Embrionario (CSF-E) desempeña un papel clave en la regulación del comportamiento neuroepitelial de las células precursoras y durante el desarrollo temprano del cerebro, suministrando señales biológicas de activación de la replicación de los precursores neuroepiteliales y de la neurogénesis, e induce específicamente la supervivencia de las células neuroepiteliales. El CSF-E es un líquido complejo que incluye, entre otros componentes proteicos, varios tipos de apolipopr...

  10. El papel de la Química en la restauración de monumentos

    OpenAIRE

    Manuel Sánchez Martínez; Dolores Elena Álvarez Gasca

    2011-01-01

    La restauración de los monumentos es una disciplina imprescindible para la conservación de los elementos físicos que dan identidad, así como para guardar las evidencias de la historia. En estas tareas de la restauración se requiere la intervención de otras disciplinas. La Química tiene un papel importante dentro de la restauración para conocer con mayor objetividad la conformación de la materia física que constituye los monumentos; gracias a ella es posible conocer la composición mineralógica...

  11. El papel de los materiales curriculares en la intervención educativa

    OpenAIRE

    2001-01-01

    En el presente artículo, el autor nos destaca el papel tan fundamental que juegan los materiales curriculares en el proceso educativo, aunque durante décadas un uso inadecuado de los mismos generó no pocos rechazos entre los partidarios de una nueva escuela que mejorara el proceso de enseñanza y aprendizaje de las nuevas generaciones. Por ello, en un primer momento, plantea la necesidad de diseñar materiales innovadores adecuados a las exigencias actuales de la sociedad y del nuevo sistema ed...

  12. El papel del ingeniero mecánico en el contexto energético actual

    OpenAIRE

    María Dolores Durán García

    2010-01-01

    En el presente trabajo se analiza, en primer término, la evolución del papel del ingeniero a través de la historia, desde sus orígenes en la Revolución Industrial hasta nuestros días; asimismo se abordan las diferentes formas en que se ha ido concibiendo la función del ingeniero mecánico y los efectos de la situación económica, tecnológica y medioambiental en estas percepciones sociales. Esta última implicación, la ambiental, sirve de base para establecer la responsabilidad actual de este pro...

  13. Inmunogenética de la enfermedad celiaca: papel de la IL-15 y su receptor

    OpenAIRE

    Escudero Hernández, Celia

    2015-01-01

    La enfermedad celiaca (EC) es una enfermedad inflamatoria sistémica y crónica provocada por las prolaminas del gluten que afecta a individuos genéticamente predispuestos (HLA-DQ2/DQ8). El único tratamiento disponible es una dieta sin gluten estricta. La interleucina (IL)-15 tiene un papel fundamental en esta enfermedad, por su potente capacidad inflamatoria y por ser el punto de intersección entre las respuestas inmunológicas adaptativa e innata. Con esta tesis, se profundizó en...

  14. Papel de la investigación cualitativa en los estudios de las relaciones internacionales

    OpenAIRE

    Fernández, Marta Elvira

    2010-01-01

    La complejidad creciente de las relaciones internacionales impone que sus estudios sean realizados con una multiplicidad de enfoques metodológicos, dentro de los cuales las perspectivas metodológicas cualitativas cumplen papeles significativos. Por ejemplo, los fundamentos culturales como subjetivos que gravitan en los diseños y ejecuciones de las relaciones de ese tipo. Por ello, en el trabajo que se presentará se realiza un esbozo de la metodología de empleo para estimar la dimensión cua...

  15. Papel vs. digital: hábitos de lectura de los estudiantes de la UEM

    OpenAIRE

    Belén García-Delgado Giménez; Gala Arias Rubio

    2014-01-01

    En este artículo se incluyen los resultados de un estudio realizado en la Facultad de Artes y Comunicación de la Universidad Europea de Madrid con los alumnos del Grado de Periodismo y Comunicación Audiovisual. El objetivo del trabajo era comprobar los hábitos de lectura en prensa de los sujetos en cuanto a soportes. De esta forma descodificaron la información contenida en el mismo número de un periódico a través de: iPad, ereaders y papel. Para la lectura de la edición digital se hizo uso de...

  16. El papel de la propiedad intelectual en las artesanías mexicanas

    OpenAIRE

    García Villicaña, Ruth

    2016-01-01

    Ante la nueva era tecnológica donde el conocimiento juega un papel trascendental como potenciador de las innovaciones, y estás a su vez representan una fuente de riqueza para los países desarrolladores, la necesidad de garantizar la obtención de beneficios económicos para los tenedores de dicha innovación se ha vuelto prioritaria, por ende, la Propiedad Intelectual (PI) ha tomado mayor relevancia; si bien es cierto que no es la única forma de protección y que ésta depende del tipo de tecnolog...

  17. O papel preditor dos estilos de apego na resolução do conflito conjugal

    OpenAIRE

    Patrícia Scheeren; Marina Zanella Delatorre; Angélica Paula Neumann; Adriana Wagner

    2015-01-01

    Este artigo avaliou o papel preditor dos estilos de apego na resolução do conflito conjugal. Foram investigados 428 homens e mulheres com idades entre 18 e 75 anos, de diferentes níveis socioeconômicos, residentes na região sul do Brasil. Os participantes responderam a um questionário sociodemográfico, ao The Conflict Resolution Style Inventory (CRSI) e à Escala de Apego Adulto (EAA). Realizou-se uma análise de regressão linear múltipla para testar as hipóteses. Os resultados apontam que os e...

  18. Aplicaciones biológicas de las fitasas: papel en los fertilizantes microbianos

    OpenAIRE

    María Teresa Fernández; Hilda Rodríguez

    2006-01-01

    La mayoría de los suelos contienen grandes cantidades de P total que aparece tanto en fracciones orgánicas como inorgánicas y debido a la fertilización con fósforo. El mayor componente de fósforo orgánico en el suelo es el fitato (ácido fítico) y es degradado por una clase de enzimas colectivamente denominadas fitasas. Los microorganismos juegan un importante papel en procesos que afectan la transformación del ácido fítico en el suelo y su disponibilidad para las plantas. Debido a la inhabili...

  19. Papel vs. digital: hábitos de lectura de los estudiantes de la UEM

    OpenAIRE

    Belén García-Delgado Giménez; Gala Arias Rubio

    2014-01-01

    En este artículo se incluyen los resultados de un estudio realizado en la Facultad de Artes y Comunicación de la Universidad Europea de Madrid con los alumnos del Grado de Periodismo y Comunicación Audiovisual. El objetivo del trabajo era comprobar los hábitos de lectura en prensa de los sujetos en cuanto a soportes. De esta forma descodificaron la información contenida en el mismo número de un periódico a través de: iPad, ereaders y papel. Para la lectura de la edición digital se hizo uso de...

  20. El papel de los agentes sociales en un contexto no lucrativo

    OpenAIRE

    Antonio Muñoz Porcar; María Jesús Alonso Nuez

    2009-01-01

    El objeto de este trabajo es contextualizar el papel de los Agentes Sociales en la economía y en las teorías explicativas del nacimiento de las Organizaciones no Lucrativas o tercer sector. Para ello, después de un análisis del origen de las ONL desde el lado de la demanda y desde la oferta, se ha realizado una encuesta a los directivos de estas organizaciones y se concluye que de las teorías que explican por el lado de la oferta la aparición de estas organizaciones son dos de ellas las que l...

  1. Interacciones neuroinmunes y dolor neuropático: papel del factor de crecimiento neuronal

    OpenAIRE

    Ruiz Barría, Guido Raúl

    2005-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada Diversas líneas de evidencia asignan al factor de crecimiento neuronal (NGF), expresado en el espacio endoneural por células de Schwann activadas tras una lesión nerviosa, un papel en el mecanismo del dolor neuropático periférico. Se ha sugerido, pues, que el NGF podría promover sprouting en la zona de la lesión y generar cambios plásticos en la primera neurona sensorial y sus proyecciones centrales, colaborando de este ...

  2. Thiophene metabolism by E. coli

    Energy Technology Data Exchange (ETDEWEB)

    Clark, D.P.

    1990-01-01

    The objective of this project is to investigate the mechanism of degradation of sulfur containing heterocyclic molecules such as those found in coal, by mutants of Escherichia coli K-12. We previously isolated multiple mutants of E. coli which were selected for improved oxidation of furan and thiophene derivatives. We have focused on the thdA mutation in our subsequent research as it appears to be of central importance in thiophene oxidation. We hope that analysis of the thd genes of E. coli will lead to improvement of our thiophene metabolizing bacterial strains. 1 tab.

  3. Wizlaw III og minnesangen

    DEFF Research Database (Denmark)

    Pontoppidan, Maria

    2012-01-01

    Artikel om den sidste slaviske Rügenfyrste, Wizlaw III (1265/68-1325), der traditionelt har været identificeret med minnesangeren Wizlaw den Unge. Om de bevarede sange og om minnesangens rolle ved det rügenske fyrstehof.......Artikel om den sidste slaviske Rügenfyrste, Wizlaw III (1265/68-1325), der traditionelt har været identificeret med minnesangeren Wizlaw den Unge. Om de bevarede sange og om minnesangens rolle ved det rügenske fyrstehof....

  4. III-V microelectronics

    CERN Document Server

    Nougier, JP

    1991-01-01

    As is well known, Silicon widely dominates the market of semiconductor devices and circuits, and in particular is well suited for Ultra Large Scale Integration processes. However, a number of III-V compound semiconductor devices and circuits have recently been built, and the contributions in this volume are devoted to those types of materials, which offer a number of interesting properties. Taking into account the great variety of problems encountered and of their mutual correlations when fabricating a circuit or even a device, most of the aspects of III-V microelectronics, from fundamental p

  5. Thiol redox requirements and substrate specificities of recombinant cytochrome c assembly systems II and III.

    Science.gov (United States)

    Richard-Fogal, Cynthia L; San Francisco, Brian; Frawley, Elaine R; Kranz, Robert G

    2012-06-01

    The reconstitution of biosynthetic pathways from heterologous hosts can help define the minimal genetic requirements for pathway function and facilitate detailed mechanistic studies. Each of the three pathways for the assembly of cytochrome c in nature (called systems I, II, and III) has been shown to function recombinantly in Escherichia coli, covalently attaching heme to the cysteine residues of a CXXCH motif of a c-type cytochrome. However, recombinant systems I (CcmABCDEFGH) and II (CcsBA) function in the E. coli periplasm, while recombinant system III (CCHL) attaches heme to its cognate receptor in the cytoplasm of E. coli, which makes direct comparisons between the three systems difficult. Here we show that the human CCHL (with a secretion signal) attaches heme to the human cytochrome c (with a signal sequence) in the E. coli periplasm, which is bioenergetically (p-side) analogous to the mitochondrial intermembrane space. The human CCHL is specific for the human cytochrome c, whereas recombinant system II can attach heme to multiple non-cognate c-type cytochromes (possessing the CXXCH motif.) We also show that the recombinant periplasmic systems II and III use components of the natural E. coli periplasmic DsbC/DsbD thiol-reduction pathway. This article is part of a Special Issue entitled: Biogenesis/Assembly of Respiratory Enzyme Complexes.

  6. Toward Network Biology in E. coli Cell.

    Science.gov (United States)

    Mori, Hirotada; Takeuchi, Rikiya; Otsuka, Yuta; Bowden, Steven; Yokoyama, Katsushi; Muto, Ai; Libourel, Igor; Wanner, Barry L

    2015-01-01

    E. coli has been a critically important model research organism for more than 50 years, particularly in molecular biology. In 1997, the E. coli draft genome sequence was published. Post-genomic techniques and resources were then developed that allowed E. coli to become a model organism for systems biology. Progress made since publication of the E. coli genome sequence will be summarized.

  7. Mechanism of Ribonuclease III Catalytic Regulation by Serine Phosphorylation

    Science.gov (United States)

    Gone, Swapna; Alfonso-Prieto, Mercedes; Paudyal, Samridhdi; Nicholson, Allen W.

    2016-05-01

    Ribonuclease III (RNase III) is a conserved, gene-regulatory bacterial endonuclease that cleaves double-helical structures in diverse coding and noncoding RNAs. RNase III is subject to multiple levels of control, reflective of its global regulatory functions. Escherichia coli (Ec) RNase III catalytic activity is known to increase during bacteriophage T7 infection, reflecting the expression of the phage-encoded protein kinase, T7PK. However, the mechanism of catalytic enhancement is unknown. This study shows that Ec-RNase III is phosphorylated on serine in vitro by purified T7PK, and identifies the targets as Ser33 and Ser34 in the N-terminal catalytic domain. Kinetic experiments reveal a 5-fold increase in kcat and a 1.4-fold decrease in Km following phosphorylation, providing a 7.4–fold increase in catalytic efficiency. Phosphorylation does not change the rate of substrate cleavage under single-turnover conditions, indicating that phosphorylation enhances product release, which also is the rate-limiting step in the steady-state. Molecular dynamics simulations provide a mechanism for facilitated product release, in which the Ser33 phosphomonoester forms a salt bridge with the Arg95 guanidinium group, thereby weakening RNase III engagement of product. The simulations also show why glutamic acid substitution at either serine does not confer enhancement, thus underscoring the specific requirement for a phosphomonoester.

  8. Ubiquity and persistance of Escherichia coli in a midwestern coastal stream

    Science.gov (United States)

    Byappanahalli, Muruleedhara N.; Fowler, Melanie; Shively, Dawn; Whitman, Richard

    2003-01-01

    Dunes Creek, a small Lake Michigan coastal stream that drains sandy aquifers and wetlands of Indiana Dunes, has chronically elevated Escherichia coli levels along the bathing beach near its outfall. This study sought to understand the sources ofE. coli in Dunes Creek's central branch. A systematic survey of random and fixed sampling points of water and sediment was conducted over 3 years. E. coliconcentrations in Dunes Creek and beach water were significantly correlated. Weekly monitoring at 14 stations during 1999 and 2000 indicated chronic loading of E. coli throughout the stream. Significant correlations between E. coli numbers in stream water and stream sediment, submerged sediment and margin, and margin and 1 m from shore were found. Median E. coli counts were highest in stream sediments, followed by bank sediments, sediments along spring margins, stream water, and isolated pools; in forest soils, E. coli counts were more variable and relatively lower. Sediment moisture was significantly correlated with E. colicounts. Direct fecal input inadequately explains the widespread and consistent occurrence of E. coli in the Dunes Creek watershed; long-term survival or multiplication or both seem likely. The authors conclude that (i) E. coli is ubiquitous and persistent throughout the Dunes Creek basin, (ii) E. coli occurrence and distribution in riparian sediments help account for the continuous loading of the bacteria in Dunes Creek, and (iii) ditching of the stream, increased drainage, and subsequent loss of wetlands may account for the chronically high E. coli levels observed.

  9. A volta do papel das crianças no contrato geracional

    Directory of Open Access Journals (Sweden)

    Jens Qvortrup

    2011-01-01

    Full Text Available El artículo presenta las relaciones entre las categorías infancia, madurez y vejez estableciendo su correlación económica, política y social. Retoma la lógica histórica sobre las relaciones sociales entre las categorías y discute las consecuencias del incremento del número de ancianos en relación a la escasez de niños, señalando los riesgos causados por ese desequilibrio. Por medio del concepto de contrato generacional, el estudio destaca el papel de la inversión en los niños y en su trabajo escolar, como una ventaja para la sociedad de manera general, una vez que los resultados de ese trabajo se harán visibles posteriormente. La argumentación procura, en especial, insertar a los niños en su papel histórico como participantes y contribuyentes de la sociedad.

  10. El papel del ingeniero mecánico en el contexto energético actual

    Directory of Open Access Journals (Sweden)

    María Dolores Durán García

    2010-01-01

    Full Text Available En el presente trabajo se analiza, en primer término, la evolución del papel del ingeniero a través de la historia, desde sus orígenes en la Revolución Industrial hasta nuestros días; asimismo se abordan las diferentes formas en que se ha ido concibiendo la función del ingeniero mecánico y los efectos de la situación económica, tecnológica y medioambiental en estas percepciones sociales. Esta última implicación, la ambiental, sirve de base para establecer la responsabilidad actual de este profesional de la Ingeniería en el consumo de energía, tanto en México como a nivel mundial y el papel que debe cumplir en el diseño de sistemas que permitan transformar y/o emplear la energía de manera racional y eficiente. Con base en lo anterior, finalmente se indican algunos de los conocimientos y habilidades que este profesional debe adquirir durante su formación profesional, para cumplir con esta importante responsabilidad.

  11. O papel político do povo na proposta republicana de Maquiavel

    Directory of Open Access Journals (Sweden)

    Murilo Pollo Martins

    2013-07-01

    Full Text Available Neste texto procuramos analisar a forma de governo republicana nas obras de Maquiavel, com destaque para o Discurso sobre as formas de governo de Florença, com o intuito de fornecer a base teórica necessária para compreender o projeto maquiaveliano de instaurar uma república em Florença, de modo que pudesse cessar os frequentes defeitos apresentados pelos regimes passados, os quais compartilhavam da característica de não considerar todos os humores ou parcelas políticas da cidade, ou seja, de favorecer apenas uma parte dela, motivo pelo qual não alcançaram uma estabilidade nas suas instituições políticas. A partir disso, nos propomos a analisar também como Maquiavel configura seu tipo de república, que possui um caráter popular, bem como o papel desempenhado pelo povo nessa república. Concluímos que na proposta de Maquiavel, embora o conflito seja inevitável, as boas leis para a cidade deverão decorrer justamente da relação antagônica das partes. Por conseguinte, o povo exerce o papel de fiscalizar as instituições e garantir a liberdade do regime político.

  12. COMUNICACIÓN EN SALUD: EL PAPEL DE LOS AGENTES COMUNITARIOS DE SALUD

    Directory of Open Access Journals (Sweden)

    Rose Marie INOJOSA

    2007-11-01

    Full Text Available El objetivo es reflejar en el papel de los agentes communitarios de la salud en la comunicación en salud. Los modelos del equipo y de la familia están en la transformación en la sociedad. Requieren nuevas formas de comunicación, capaces de comtemplar esta dinámica, la movilidad y las interacciones de los papeles sociales. Lo Programa de Salud de la Família (PSF busca hacer una inversión importante en la lógica y en las prácticas implicadas en el proceso de la producción de la salud, trabajando con las familias y los equipos como protagonistas, en un plan horizontal. El Programa encuentra, en este proceso, las trampas de los hábitos de las viejas organizaciones. La comunicación autoritaria construye paredes entre los ciudadanos. El Programa encuentra, también, la influencia del marketing en la comunicación diaria. Sin embargo carga posibilidades innumerables para generar comprensión, confianza y solidaridad. Algunas posibilidades habían sido intentadas y evaluadas ya. Reiteran la capacidad del modelo del PSF en ocuparse de la dinámica del contemporáneo de la sociedad.

  13. O papel dos gestores na construção da moralidade

    Directory of Open Access Journals (Sweden)

    Marina Novaes de Senne

    2014-02-01

    Full Text Available A presente comunicação tem como objetivo pensar a moralidade na escola, no contexto do relativismo ético contemporâneo. O foco de nossa análise é o papel dos gestores na construção desses valores. Utilizamos a concepção de educação moral que considera os valores como investimentos afetivos capazes de serem formados no espaço escolar. Essa corrente teórica foi revista a partir das transformações da sociedade contemporânea com os autores Puig (1998, La Taille (2009 e Araújo (2007. Este trabalho dividiu-se em três momentos distintos. O primeiro deles problematiza a educação moral na atualidade. No segundo momento, matizamos acerca de caminhos possíveis para a construção da moral. Por fim, pensamos no papel dos gestores que atuam entre a lei e os educadores. Foi possível concluir que os gestores têm uma contribuição central na construção da moral na escola, pois devem ajudar a construir os espaços de debate, assim como criar uma escola que possibilite a vivência na moralidade.

  14. Método experimental para consolidar fibras de papel. (I parte del estudio

    Directory of Open Access Journals (Sweden)

    Alicia De Lera Santín

    2009-08-01

    Full Text Available En este artículo describimos un nuevo método enzimático para consolidar la celulosa del papel. Este método consiste en la síntesis de la celulosa con una enzima hidrolítica: la celulasa. Ésta actúa como una sintetasa, consolidando el papel cuando es mezclada en una solución orgánica de solvente/tampón (4:1 en presencia de un sustrato (β-cellobiosyl fluoride. Para mejorar el tratamiento añadimos un gel de agar-agar con todos los reactivos necesarios. Para observar el estado de las fibras antes y después del tratamiento, realizamos test mecánicos y fotografías de microscopía electrónica de barrido. Se observó que el proceso de consolidación casi consigue recuperar el estado físico inicial de las fibras así como su resistencia al desgarre y alargamiento.

  15. O papel do enfermeiro de saúde publica: projeções no ensino

    Directory of Open Access Journals (Sweden)

    Bertha Cruz Enders

    1995-09-01

    Full Text Available Analisa-se o papel do enfermeiro em saúde pública no contexto do ensino, como ponto de partida para uma avaliação da prática. Objetivou-se 1 Identificar o papel do enfermeiro em saúde pública projetado nos conteúdos programáticos das disciplinas de saúde pública do Curso de Enfermagem na Universidade Federal do Rio Grande do Norte desde sua implantação em 1974 até 1990 e, 2 Avaliar as concepções projetadas no ensino frente a realidade das práticas dos egressos. Conceptualizações da teoria de papeis nortearam o entendimento do termo "papel" e do processo de internalização do conceito. Utilizou-se uma abordagem qualitativa com triangulação na coleta de dados. Dados foram obtidos em duas fontes: nos conteúdos programáticos do Curso e nas opiniões dos enfermeiros egressos do Curso. Técnicas de análise documental foram aplicadas a 70 programas de disciplinas de Saúde Pública e uma amostra aleatória estratificada de 60 enfermeiros foi entrevistada utilizando um questionário específico. Os dados foram analisados à luz da teoria de papeis para a identificação do conceito. Em seguida, se analisou o conceito com base nas políticas de saúde vigentes da época em estudo. Os resultados demonstram que o papel do enfermeiro em saúde pública projetado nos conteúdos focaliza funções múltiplas, assistenciais educativas e administrativas dentro de uma visão preventiva. O objetivo da enfermagem em saúde pública tem sido a prevenção, dentro de uma prática de assistência primária e mais recentemente, dentro de uma perspectiva de mudança e de transformação das questões sociais e das políticas de saúde. Focaliza o enfermeiro planejador, competente nas técnicas epidemiológicas. A percepção dos egressos é também preventiva e focaliza o papel educador e conscientizador do enfermeiro, embora pouco avanço se observa com relação às questões sociais da população. Existe uma incompatibilidade entre os

  16. Escherichia coli Uropathogenesis In Vitro

    DEFF Research Database (Denmark)

    Andersen, Thomas E; Khandige, Surabhi; Madelung, Michelle

    2012-01-01

    Uropathogenic Escherichia coli (UPEC) strains are capable of invading bladder epithelial cells (BECs) on the bladder luminal surface. Based primarily on studies in mouse models, invasion is proposed to trigger an intracellular uropathogenic cascade involving intracellular bacterial proliferation...

  17. A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode

    NARCIS (Netherlands)

    Jergic, Slobodan; Horan, Nicholas P.; Elshenawy, Mohamed M.; Mason, Claire E.; Urathamakul, Thitima; Ozawa, Kiyoshi; Robinson, Andrew; Goudsmits, Joris M. H.; Wang, Yao; Pan, Xuefeng; Beck, Jennifer L.; van Oijen, Antoine M.; Huber, Thomas; Hamdan, Samir M.; Dixon, Nicholas E.

    2013-01-01

    Processive DNA synthesis by the alpha epsilon theta core of the Escherichia coli Pol III replicase requires it to be bound to the beta(2) clamp via a site in the a polymerase subunit. How the epsilon proofreading exonuclease subunit influences DNA synthesis by alpha was not previously understood. In

  18. Implementación de fábrica de papel y derivados empleando residuos de banano como materia prima

    OpenAIRE

    Davalos Funes, Maria Paulina; Zurita Erazo, Sonia Analia

    2009-01-01

    El sector papelero presenta niveles de riesgo. La industria que importa pulpa de papel está a merced de los precios internacionales, pero el mayor problema es a nivel ecológico. El mercado internacional exige que la industria papelera implemente Normas internacionales “ISO 14000” para garantizar una mejor “Gestión Ambiental”. La industria papelera, para su proceso utiliza una gran cantidad de árboles lo que hace evidente que en el corto plazo no podrán producir papel a base de la pulpa, debem...

  19. El papel de la inteligencia y de la metacognición en la resolución de problemas

    OpenAIRE

    Domenech Auqué, Montserrat

    2004-01-01

    El objetivo de esta tesis doctoral es estudiar: a) el papel de la inteligencia en la resolución de problemas, b) la relación entre la inteligencia y la metacognición y c) el papel de la metacognición en la resolución de problemas. La muestra ha estado formada por n=140 alumnos de Secundaria entre 16 y 18 años, a los que se les administraron pruebas formales (Test de Aptitudes Diferenciales y Test de Torrance de Pensamiento Creativo) e informales (las Escalas de Renzulli (SRBCSS) y una Autobio...

  20. (Re) pensando o papel do educador especial no contexto da inclusão de alunos surdos

    OpenAIRE

    Cinara Franco Rechico; Vera Lucia Marostega

    2012-01-01

    A presente pesquisa refere-se a uma das questões mais discutidas, atualmente, na área da Educação Especial: a Escola Inclusiva. Percebe-se que na caminhada de uma educação para todos e sem exclusão, é fundamental que o educador especial tenha seu papel definido para que assim esteja consciente da sua prática pedagógica. Busca-se, por meio desta, (re)pensar o papel do educador especial na classe do sistema regular de ensino em que haja inclusão de alunos surdos. Esta pesquisa envolve como suje...

  1. Efectos de un programa de kickboxing educativo sobre el papel importante del alumnado. Percepciones de estudiantes y profesorado

    OpenAIRE

    Menéndez Santurio, J.I.; Fernández-Río, J.

    2017-01-01

    El objetivo del estudio fue conocer las percepciones de alumnado y profesorado sobre un programa de kickboxing educativo (sin contacto) y su efecto sobre el papel importante del primero. Participaron 60 alumnos (15,4 ± ,90 años) y dos profesores. Un diseño pre-experimental con medidas pretest-postest y un estudio de caso fue usado. Instrumentos de recogida de datos: subescala del papel importante del Cuestionario del Clima Motivacional Percibido, pregunta abierta a todos los estudiantes, phot...

  2. Inhibition of expression of virulence genes of Yersinia pestis in Escherichia coli by external guide sequences and RNase P.

    Science.gov (United States)

    Ko, Jae-hyeong; Izadjoo, Mina; Altman, Sidney

    2008-08-01

    External guide sequences (EGSs) targeting virulence genes from Yersinia pestis were designed and tested in vitro and in vivo in Escherichia coli. Linear EGSs and M1 RNA-linked EGSs were designed for the yscN and yscS genes that are involved in type III secretion in Y. pestis. RNase P from E. coli cleaves the messages of yscN and yscS in vitro with the cognate EGSs, and the expression of the EGSs resulted in the reduction of the levels of these messages of the virulence genes when those genes were expressed in E. coli.

  3. Antibacterial Activity and Cytotoxicity of Gold (I) and (III) Ions and Gold Nanoparticles

    OpenAIRE

    Shareena Dasari, TP; Zhang, Y; Yu, H.

    2015-01-01

    Gold nanoparticles (AuNPs) and gold ion complexes have been investigated for their antibacterial activities. However, the majority of the reports failed to disclose the concentration of free Au(I) or Au(III) present in solutions of AuNPs or gold ion complexes. The inconsistency of antibacterial activity of AuNPs may be due to the effect of the presence of Au(III). Here we report the antibacterial activity of Au(I) and Au(III) to four different bacteria: one nonpathogenic bacterium: E. coli an...

  4. Biotecnología en la industria de la pulpa y el papel: mapeo de patentes

    Directory of Open Access Journals (Sweden)

    Laura Emilia Cerón Rincón

    2010-08-01

    Full Text Available La biotecnología se plantea como una oportunidad para los países en desarrollo, Colombia cuenta con condiciones que favorecen el desarrollo de esta área, como una fuerte tradición en infraestructura básica de investigación principalmente en los campos de agricultura y la salud humana. Las iniciativas regionales han ayudado a levantar el perfil de la industria biotecnológica como: El Cluster de Bioindustrial del Valle del Cauca, El ejercicio Prospectivo en Biotecnología y Salud de Antioquia y La iniciativa de Bioprogreso en la región Bogotá-Cundinamarca. Según la Cámara de Comercio de Bogotá (2005 se consi-deran a la cadena productiva del papel y artes gráficas como estratégicas para la región de Bogotá D.C.Según la visión de la Agenda Interna para la Productividad y la Competitividad del Departamento Nacional de Planeación (2007 para dicha cadena, esta alcanzará progresivamente mayor competitividad y mayor inserción en el mercado internacional globalizado, llegando a triplicar sus exportaciones para el año 2015, entre las acciones identificadas para lograr este objetivo el se encuentra el Desarrollo tecnológico, donde se propone aprovechar los avances en biotecnología. Dado esto, se recopilaron patentes que incluyeran desarrollos biotecnológicos para la industria de la pulpa y el papel del año 2000 hasta la fecha, a partir de las cuales se realizo un análisis de la información recopilada a través de una herramienta de vigilancia tecnológica con la que generó un mapa tecnológico, como un primer paso de un proceso dirigido a contribuir en la definición de políticas y direccionamiento de la gestión tecnológica para que la cadena de papel.

  5. A GEOGRAFIA DO MERCADO MUNDIAL DE PRODUTOS AGROALIMENTARES E O PAPEL DO BRASIL

    Directory of Open Access Journals (Sweden)

    Marlon Clovis Medeiros

    2014-02-01

    Full Text Available O artigo analisa as mudanças recentes na geografia do mercado mundial de produtos agrícolas, discutindo a inserção do Brasil, o papel dos mercados futuros na elevação dos preços internacionais e as mudanças entre os países exportadores e importadores de produtos agrícolas. Argumentamos que a principal mudança no espaço agrícola mundial nas últimas duas décadas foi o crescimento dos investimentos financeiros em commodities agrícolas e o crescimento dos mercados de futuros. A dinâmica financeira se tornou dominante nos mercados agrícolas, levando à mudanças nas safras e nos preços, tornando-os subordinados à expectativa de retorno dos investidores. Este foi o principal elemento da inflação de preços agrícolas em 2006-2008. Estudos dos mais diversos organismos internacionais (FAO, FMI e Banco Mundial apontaram cenários de agravamento da fome em inúmeros países e de continuidade da alta de preços, mas desviaram o foco do papel da especulação, apontando duas grandes causas: a produção de biocombustíveis e o aumento do consumo de alimentos na Ásia. No presente artigo argumentamos pelo contrário, que o papel das vendas antecipadas nas bolsas de mercadorias e futuros, foi o elemento determinante. A oferta e a demanda agrícola vem perderam espaço como reguladores de preços para a antecipação e expectativas dos mercados futuros. Os preços são determinados antes mesmo do plantio. A ampliação dos fluxos internacionais de capital e as crises financeiras trouxeram a dinâmica internacional para o centro dos debates geográficos e sobre os rumos da agricultura.

  6. Diarrheagenic Escherichia coli Markers and Phenotypes among Fecal E. coli Isolates Collected from Nicaraguan Infants ▿

    OpenAIRE

    Reyes, Daniel; Vilchez, Samuel; Paniagua, Margarita; Colque-Navarro, Patricia; Weintraub, Andrej; Möllby, Roland; Kühn, Inger

    2010-01-01

    We analyzed the prevalence of diarrheagenic Escherichia coli (DEC) markers and common phenotypes in 2,164 E. coli isolates from 282 DEC-positive samples. Enteropathogenic E. coli (EPEC) and enteroaggregative E. coli (EAEC) were very diverse and were not correlated with diarrhea. Enterotoxigenic E. coli (ETEC) estA and enterohemorrhagic E. coli (EHEC) belonged to a few phenotypes and were significantly correlated with diarrhea.

  7. Diarrheagenic Escherichia coli Markers and Phenotypes among Fecal E. coli Isolates Collected from Nicaraguan Infants ▿

    OpenAIRE

    2010-01-01

    We analyzed the prevalence of diarrheagenic Escherichia coli (DEC) markers and common phenotypes in 2,164 E. coli isolates from 282 DEC-positive samples. Enteropathogenic E. coli (EPEC) and enteroaggregative E. coli (EAEC) were very diverse and were not correlated with diarrhea. Enterotoxigenic E. coli (ETEC) estA and enterohemorrhagic E. coli (EHEC) belonged to a few phenotypes and were significantly correlated with diarrhea.

  8. Role of ferritin in the rice tolerance to iron overload Papel da ferritina na tolerância de arroz ao excesso de ferro

    Directory of Open Access Journals (Sweden)

    Vivian Chagas da Silveira

    2009-08-01

    condições de alta disponibilidade de ferro já foi descrito em algumas espécies vegetais. Entretanto, o papel da ferritina no mecanismo de tolerância de plantas de arroz ao excesso de ferro não é conhecido. Neste trabalho, expressamos ferritina de arroz em E. coli, produzimos um anticorpo policlonal anti-ferritina de arroz e este foi utilizado para avaliar o acúmulo de ferritina em dois cultivares de arroz (Oryza sativa considerados suscetível (BR-IRGA 409 e tolerante (EPAGRI 108 ao excesso de ferro. O anticorpo foi capaz de reconhecer ferritina purificada de sementes de ervilha, assim como ferritina de folhas de arroz. Aumentos nos níveis de mRNA e proteína foram observados nos dois cultivares sob excesso de ferro, com maior acúmulo da proteína no cultivar EPAGRI 108. Quando submetidas a excesso do elemento, plantas deste mesmo cultivar atingiram concentrações de Fe mais baixas do que plantas do cultivar BR-IRGA409, principalmente nas partes aéreas. Sugere-se que o mecanismo de tolerância ao excesso de ferro no cultivar EPAGRI 108 inclui limitação da translocação de Fe e aumento do acúmulo de ferritina. Este é o primeiro trabalho que mostra maior acúmulo da proteína ferritina em um cultivar de Oryza sativa tolerante ao excesso de Fe, fornecendo evidência de um possível papel desta proteína nos mecanismos de tolerância a este metal.

  9. Calculus III essentials

    CERN Document Server

    REA, Editors of

    2012-01-01

    REA's Essentials provide quick and easy access to critical information in a variety of different fields, ranging from the most basic to the most advanced. As its name implies, these concise, comprehensive study guides summarize the essentials of the field covered. Essentials are helpful when preparing for exams, doing homework and will remain a lasting reference source for students, teachers, and professionals. Calculus III includes vector analysis, real valued functions, partial differentiation, multiple integrations, vector fields, and infinite series.

  10. Synthesis, Characterization and Antibacterial Studies of N-(Benzothiazol-2-yl-4-chlorobenzenesulphonamide and Its Neodymium(III and Thallium(III Complexes

    Directory of Open Access Journals (Sweden)

    Lawrence Nnamdi Obasi

    2017-02-01

    Full Text Available N-(Benzothiazol-2-yl-4-chlorobenzenesulphonamide (NBTCS was synthesized by condensation reaction of 4-chlorobenzenesulphonyl chloride and 2-aminobenzothiazole in acetone under reflux. Neodymium(III and thallium(III complexes of the ligand were also synthesized. Both ligand and metal complexes were characterized using UV-Vis, IR, 1H- and 13C-NMR spectroscopies, elemental analysis and molar conductance measurement. IR studies revealed that the ligand is tridentate and coordinates to the metal ions through nitrogen and oxygen atoms of the sulphonamide group and nitrogen atom attached to benzothiazole ring. The neodymium(III complex displays a coordination number of eight while thallium(III complex displays a coordination number of six. The ligand and its complexes were screened in vitro for their antibacterial activities against Escherichia coli strains (E. coli 6 and E. coli 13, Proteus species, Staphylococcus aureus and Pseudomonas aeruginosa using the agar well diffusion technique. The synthesized compounds were found to be more active against the microorganisms screened relative to ciprofloxacin, gentamicin and co-trimoxazole.

  11. Efecto sinérgico del aceite esencial de Origanum vulgare a la Gentamicina en cultivos de Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Luis Chávez Torres

    2008-01-01

    Full Text Available Objetivo: El presente trabajo tuvo como propósito determinar el efecto sinérgico antibacteriano entre el aceite esencial de Origanum vulgare y la Gentamicina en aislados de Escherichia coli. Diseño: Estudio experimental Lugar: Centro Latinoamericano de Enseñanza e Investigación en Bacteriología Alimentaria (CLEIBA Participantes: Placas Petri conteniendo E. Coli ATCC 25922, aceite esencial de orégano y Gentamicina. Intervenciones: Se aplicó el método de Kirby Bauer (discos de difusión en 20 placas Petri. Se aisló la cepa Escherichia coli ATCC 25922. El grupo Experimental fue tratado con discos de papel filtro, embebidos con Gentamicina y aceite esencial de orégano al 75%; mientras que el grupo Control, con discos de Gentamicina sola. Se realizó la medición de los halos y se registraron los datos. Principales medidas de resultados: Se evaluó el diámetro de los halos de inhibición Resultados: Los halos de inhibición del grupo Experimental resultaron 22,375 mm., mayores que los del grupo Control (20,75 mm. La prueba T determinó que la diferencia era estadísticamente significativa, p = 0,001 (p < 0,05. Conclusiones: Existe un efecto sinérgico antibacteriano in vitro entre el aceite esencial de Origanum vulgare y la gentamicina en E. coli.

  12. EFECTO SINÉRGICO DEL ACEITE ESENCIAL DE Origanum vulgare A LA GENTAMICINA EN CULTIVOS DE Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Luis Chávez Torres

    2012-02-01

    Full Text Available Objetivo: El presente trabajo tuvo como propósito determinar el efecto sinérgico antibacteriano entre el aceite esencial de Origanum vulgare y la Gentamicina en aislados de Escherichia coli. Diseño: Estudio experimental Lugar: Centro Latinoamericano de Enseñanza e Investigación en Bacteriología Alimentaria (CLEIBA Participantes: Placas Petri conteniendo E. Coli ATCC 25922, aceite esencial de orégano y Gentamicina. Intervenciones: Se aplicó el método de Kirby Bauer (discos de difusión en 20 placas Petri. Se aisló la cepa Escherichia coli ATCC 25922. El grupo Experimental fue tratado con discos de papel filtro, embebidos con Gentamicina y aceite esencial de orégano al 75%; mientras que el grupo Control, con discos de Gentamicina sola. Se realizó la medición de los halos y se registraron los datos. Principales medidas de resultados: Se evaluó el diámetro de los halos de inhibición Resultados: Los halos de inhibición del grupo Experimental resultaron 22,375 mm., mayores que los del grupo Control (20,75 mm. La prueba T determinó que la diferencia era estadísticamente significativa, p = 0,001 (p < 0,05. Conclusiones: Existe un efecto sinérgico antibacteriano in vitro entre el aceite esencial de Origanum vulgare y la gentamicina en E. coli.

  13. Emprendedores sociales y su papel en el desarrollo integral de la comunidad

    Directory of Open Access Journals (Sweden)

    África del Valle Calánchez

    2015-01-01

    Full Text Available El propósito de la investigación es dar a conocer como los voceros y voceras de los consejos comunales logran un desarrollo integral de la co- munidad, asumiendo un papel protagónico como emprendedores sociales. El tipo de estudio fue Investigación acción participativa. El desarrollo del tema incluye aspectos como: emprendedor social, emprendimiento social a través de organizaciones socioproductivas, empoderamiento como pro- ceso de participación en las comunidades y plan comunitario de desarrollo integral. Se pudo concluir que a través de la ejecución de proyectos comu- nitarios los voceros y voceras participan en actividades de emprendimien- to social; aunque en muchos casos no son sostenibles.

  14. O papel de imunoglobulinas na nefropatia da leptospirose em suínos

    OpenAIRE

    Larissa M.F. Gonçalves; Carvalho,Sônia M. de; Ângela P. Campos; Micherlene S. Carneiro; Silva,Elis R.D.F.S.; Francisco A. L. Costa; Vanessa De Castro

    2014-01-01

    A leptospirose é uma antropozoonose endêmica em todo o mundo, que afeta o homem e várias espécies de animais domésticos e silvestres. No início da infecção há produção de IgM para o controle da infecção e após alguns dias, IgG são produzidas e provocam lise das leptospiras circulantes. Objetivou-se neste estudo identificar depósitos de antígeno de leptospiras e imunoglobulinas no tecido renal, para avaliar o papel de imunoglobulinas na patogênese da nefropatia da leptospirose em suínos. Foram...

  15. O PAPEL DO BNDES NA INTEGRAÇÃO DO TERRITÓRIO BRASILEIRO

    Directory of Open Access Journals (Sweden)

    Hélio Caetano Farias

    2013-08-01

    Full Text Available Este artigo busca analisar o papel do BNDES na industrialização e na integração do território brasileiro. Criado na década de 50, o Banco tornou-se uma das principais referências de planejamento da burocracia estatal e, imprescindível, aos principais projetos nacionais das mais diversas orientações. Na década de 90, o BNDES foi estratégico na elaboração e operacionalização de um quadro normativo favorável à internacionalização do território.

  16. El conocimiento como bien común y el papel de las bibliotecas

    Directory of Open Access Journals (Sweden)

    Giovanni Solimine

    2012-02-01

    Full Text Available Parte de este texto fue presentado por el autor en la conferencia inaugural del V Encuentro Ibérico EDICIC (Asociación de Educación e Investigación en Ciencia de la Información de Iberoamérica y el Caribe, Badajoz 17-19 de noviembre de 2011. Después de analizar los problemas de acceso al conocimiento y la relación entre el conocimiento y el bienestar en la sociedad contemporánea, se analizan el papel de las bibliotecas como un servicio público y un instrumento de inclusión social. 

  17. El papel del travestismo en el pensamiento político de Judith Butler

    Directory of Open Access Journals (Sweden)

    Mariela Nahir Solana

    2014-11-01

    Full Text Available El objetivo de este artículo es examinar el papel del travestismo en la obra temprana de Judith Butler. Para ello, se mostrará la importancia del análisis de las actuaciones travestis en El género en disputa en relación con su concepción de la performatividad de género y de la subversión política. A su vez, se evaluarán algunas críticas a su pensamiento político, en general, y a su tratamiento de la parodia drag, en particular, y se demostrará de qué modo ambos tópicos han evolucionado en su libro posterior

  18. Los desastres de origen natural en México: el papel del FONDEN

    Directory of Open Access Journals (Sweden)

    Juan Manuel Rodríguez Esteves

    2004-01-01

    Full Text Available Por su ubicación geográfica, México es escenario de la manifestación de varios tipos de fenómenos naturales. Los huracanes y los sismos son quizás las principales fuentes de amenaza que enfrenta el territorio nacional y cuya intensidad puede ocasionar desastres. El impacto que los desastres de origen natural pueden llegar a causar debe considerarse en la planeación de los centros de población. El presente trabajo aborda las consecuencias que han tenido los desastres de origen natural en México en los últimos años. Ante la “inevitabilidad” de la manifestación de estos fenómenos, se analiza el papel que ha jugado el Fondo Nacional de Desastres Naturales (FONDEN como instrumento financiero para atender a la población afectada por los mismos

  19. El papel de los agentes sociales en un contexto no lucrativo

    Directory of Open Access Journals (Sweden)

    Antonio Muñoz Porcar

    2009-01-01

    Full Text Available El objeto de este trabajo es contextualizar el papel de los Agentes Sociales en la economía y en las teorías explicativas del nacimiento de las Organizaciones no Lucrativas o tercer sector. Para ello, después de un análisis del origen de las ONL desde el lado de la demanda y desde la oferta, se ha realizado una encuesta a los directivos de estas organizaciones y se concluye que de las teorías que explican por el lado de la oferta la aparición de estas organizaciones son dos de ellas las que lo explican: teoría de los grupos de poder y teoría de apoyos del sector público.

  20. Redefinições no papel do Estado e a política educacional brasileira

    Directory of Open Access Journals (Sweden)

    Vera Maria Vidal Peroni

    2015-10-01

    Full Text Available En este artículo se pretende analizar cómo la redefinición del papel del Estado en la reorganización de los límites entre público y privado se materializan en la política brasileña de educación a través de la asociación del sector público con el mercado del tercer sector, con implicaciones para la democratización de la educación. El texto presenta una breve historia de la educación brasileña, un ejemplo de una asociación entre el sector público y el tercero, el Instituto Ayrton Senna y un sistema público de educación básica representada por una escuela pública del estado de Rio Grande do Sul y el debate sobre la redefinición del papel del Estado que se produjo y el actual contexto de cambios en la configuración entre el sector público y privado. AbstractThis article aims to analyze how the redefinitions of the state role in reorganizing the boundaries between public and private materialize in the Brazilian educational policy through the partnership of the public sector with the third sector market, with implications for the democratization of education. The text presents a brief history of Brazilian education, an example of a partnership between the public and third sector, the Ayrton Senna Institute and a public system of basic education represented by a public school of the state of Rio Grande do Sul and debate about redefining the role of the state occurred and the current context of changes in settings between the public and private. SumárioO artigo Redefinições no papel do Estado e a política educacional brasileira tem como objetivo analisar as conexões entre o público e o privado, enfocando o contexto da relação entre o diagnóstico neoliberal, incorporado pela Terceira Via, de que o “culpado” pela crise é o Estado, e as estratégias que propõem o deslocamento da execução das políticas sociais do Estado para a sociedade, e o que fica como propriedade do Estado adquire a

  1. Papel das células T reguladoras no desenvolvimento de dermatoses

    OpenAIRE

    Lima,Hermênio Cavalcante

    2006-01-01

    Células T, em particular as células T CD4+, têm sido associadas a muitos aspectos das doenças de pele. A evidência atual sugere, porém, que o papel dos linfócitos T CD4+ no desenvolvimento de inflamação cutânea excede o de ativador pró-inflamatório das células T de ação que dirigem a resposta imune. Subtipos de células T com capacidade reguladora, tais como Tregs CD4+CD25+high, têm sido identificadas. Observações recentes sugerem que em algumas doenças da pele a função dessas células está mod...

  2. EL PAPEL MODERADOR DE LA CULTURA EN LA GENERACION DE SATISFACCIÓN Y LEALTAD

    Directory of Open Access Journals (Sweden)

    Medina Molina, Cayetano

    2011-01-01

    Full Text Available "El presente trabajo analiza las diferencias en el funcionamiento del modelo explicativo del desarrollo de satisfacción y lealtad por parte del turista entre dos grupos culturales distintos (individualistas con baja aversión a la incertidumbre, y colectivistas con alta aversión a la incertidumbre, así como la posible existencia de variables mediadoras y moderadoras en la relaciónentre satisfacción y lealtad. Específicamente, el modelo cognitivo-afectivo utilizado contempla el papel jugado por las expectativas ajustadas como mediadoras entre la satisfacción del consumidor y su lealtad. Los resultados obtenidos indican la existencia de diferencias relevantes en el comportamiento de los dos grupos culturales analizados."

  3. El papel del lenguaje en las estrategias discursivas para fomentar la enseñanza-aprendizaje

    Directory of Open Access Journals (Sweden)

    LUIS FERNANDO NIETO RUIZ

    2011-01-01

    Full Text Available Mediante estos breves acápites se hace una reflexión en torno del lenguaje y su papel en la formación del ser humano en valores, principios, conocimientos y relaciones interpersonales. Es aquí en donde la educación relacionada con el oficio del maestro se debe centrar en la propalación de las competencias como la propositiva, la interpretativa y la argumentativa; además de esto, que contribuya con la formación de un ser con sentimientos y valores, para que se respete a sí mismo y, por tanto, a los demás, que tenga la capacidad de pensar y reflexionar sobre los diferentes problemas de su entorno. Todo lo anterior se logra mediante la puesta en escena de las estrategias discursivas basadas en la argumentación.

  4. EL PAPEL DE LA DISTANCIA PSÍQUICA Y LA COMPLEJIDAD PARA EVALUARLA

    Directory of Open Access Journals (Sweden)

    Eliseo Emmanuel González Guzmán

    2016-01-01

    Full Text Available Resumen En este trabajo se revisa el concepto de distancia psíquica utilizado en el modelo de internacionalización de Uppsala, se destaca su papel fundamental y la dificultad de medición en investigaciones empíricas resultado de la amplitud de los términos que engloba, para esto se realiza un análisis exploratorio de clúster jerárqui - co sobre algunas variables que comúnmente se utilizan para medir la distancia psíquica entre países. Esta investigación está enfocada en el estudio de la distancia psíquica de los países destino de las exportaciones de las empresas muebleras mexicanas registradas en el Directorio de Exportadores (DIEX de la Secretaría de Economía (2015.

  5. NORMAS Y PRINCIPIOS DE AUDITORÍA APLICABLES EN LA EVIDENCIA Y PAPELES DE DE TRABAJO

    OpenAIRE

    Zanabria Huisa, Elmer; Facultad de Ciencias Contables, Universidad Nacional Mayor de San Marcos

    2014-01-01

    Previamente señalaré que las Normas y Principios de Auditoría son los lineamientos básicos que el auditor debe seguir en el cumplimiento de su labor de auditoría. El trabajo de algunas firmas de auditoría y de los auditores independientes ha sido y seguirá siendo objeto de severas críticas por parte de los usuarios y de la ciudadanía en cuanto al tema "obtención de evidencia y formulación de papeles de trabajo".Este problema se acentúa por el desconocimiento e inobservancia de las normas de...

  6. El papel de la educación en situaciones de posconflicto: estrategias y recomendaciones

    Directory of Open Access Journals (Sweden)

    Armando Infante Márquez

    2014-01-01

    Full Text Available En este artículo se analizaron las experiencias de posconflicto en tres países: Bosnia y Herzegovina, El Salvador y Sierra Leona, con el fin de extraer algunas enseñanzas que puedan ser útiles para el caso de Colombia, que en este momento se encuentra en conversaciones con la guerrilla para ponerle fin al conflicto armado. También se quiere mostrar el papel tan importante que desempeña la educación en la reconstrucción posconflicto, ya que provee protección física, psicosocial y cognitiva. Además, se estudiaron algunas estrategias a corto y largo plazo, con el fin de poderlas combinar de una manera razonable para lograr el desarrollo social, político y económico del país.

  7. Informaciones políticas (1975-1979: el “Parlamento de papel

    Directory of Open Access Journals (Sweden)

    Domingo García Ramos

    2012-06-01

    Full Text Available La prensa escrita desempeñó un importante protagonismo en el proceso de la transición a la democracia en España. Durante los primeros años de los setenta el diario Informaciones representó la apuesta más clara por una salida democrática al régimen de Franco desde una perspectiva liberal y reformista. Desde 1975 el periódico publicó el suplemento "Informaciones Políticas", donde pudieron exponerse libremente las opiniones de los más importantes analistas y dirigentes políticos, muchos de ellos todavía en situación de ilegalidad. Este artículo pretende resaltar la contribución del suplemento como expresión de lo que entonces se denominó "el parlamento de papel" y su influencia en la configuración de lo que luego sería el "centro democrático".Palabras clave: prensa, transición, Informaciones Políticas__________________AbstractThe press was involved in the process of transition to democracy in Spain. During early 1970, the daily information clearly defended the democratic choice of the Franco regime in a liberal and reformist perspective. Since 1975, the newspaper published the supplement "Informaciones Políticas", setting forth the views of leading analysts and political leaders, many still in an illegal situation. This article seeks to highlight the contribution of the supplement as an expression of what was then called "el parlamento de papel". Its influence in shaping what would become the "centro democrático".Keywords: press, transition, Informaciones Políticas

  8. Papel emergente do eixo GH/IGF-I no controle cardiometabólico

    Directory of Open Access Journals (Sweden)

    Carla R.P Oliveira

    2011-11-01

    Full Text Available O hormônio de crescimento (GH, principal regulador do crescimento pós-natal, tem importantes ações metabólicas em diferentes tecidos, sinérgicas ou até antagônicas às do fator de crescimento semelhante à insulina tipo I (IGF-I, produzido sobretudo no fígado após ligação do GH ao seu receptor. Experimentos em modelos animais indicam um papel importante do GH na resistência a insulina, enquanto o papel do IGF-I nessa condição ainda não está completamente elucidado. Em humanos, o GH promove aumento da lipólise e da oxidação lipídica, enquanto o IGF-I desencadeia o aumento da oxidação lipídica apenas cronicamente. Enquanto as ações sobre o crescimento são tempo limitado, as ações metabólicas e cardiovasculares do eixo GH/IGF-I perduram durante toda a vida. Os potenciais efeitos anabólicos do GH têm sido utilizados em condições crônicas e hipercatabólicas, embora as investigações sobre os desfechos clínicos ainda sejam escassas. Neste artigo, pretendemos revisar as ações metabólicas do GH oriundas de modelos animais, os estudos em humanos normais e indivíduos com deficiência de GH, diabete melito tipo 1, síndrome metabólica, estados hipercatabólicos e a relação do eixo GH/IGF-I com as adipocinas, disfunção endotelial e aterogênese

  9. EL PAPEL DE LOS VALORES EN EL DESARROLLO DE LA IDENTIDAD CORPORATIVA

    Directory of Open Access Journals (Sweden)

    Fanny Ramírez

    2005-07-01

    Full Text Available Actualmente son escasas las instituciones que cuentan con directivos conscientes de su responsabilidad de desarrollar una identidad corporativa sólida, y esto constituye una gran debilidad que está relacionada con la ausencia de valores que se requieren como elementos constituyentes de sus principios básicos (PBO. Como las demás actividades del hombre, la identidad corporativa, implica una dimensión ética que le garantice un sentido de perennidad en tanto compromete valores societales que interactúen con los valores deontológicos, organizacionales e individuales. Pero, las diferentes tipologias sobre valor realizadas exigen una revisión para establecer lo que se entiende por valor y el papel que estos juegan en el desarrollo de la identidad corporativa. Mediante un estudio de carácter bibliográfico documental cualitativo, se concluyó que los valores son capacidades comportadas por la naturaleza de los entes que pueden ser adquiridos y desarrolladas mediante el cimiento de la virtud y que la puesta en acción de los valores es una responsabilidad de la alta directiva El papel de los valores en el desarrollo de la identidad corporativa supone tres momentos o etapas de la identidad corporativa. La etapa del diseño y la de la difusión de los (PBO; el simbolismo o Identidad Visual y el comportamiento organizacional. La puesta en práctica de todos esos valores permitirá en la tercera etapa la identificación.

  10. Functional cooperation of the dnaE and dnaN gene products in Escherichia coli.

    OpenAIRE

    Kuwabara, N; Uchida, H.

    1981-01-01

    A system was designed to isolate second-site intergenic suppressors of a thermosensitive mutation of the dnaE gene of Escherichia coli. The dnaE gene codes for the alpha subunit of DNA polymerase III [McHenry, C. S. & Crow, W. (1979) J. Biol. Chem. 254, 1748-1753]. One such suppressor, named sueA77, was finely mapped and found to be located at 82 min on the E. coli chromosome, between dnaA and recF, and within the dnaN gene [Sakakibara, Y. & Mizukami, T. (1980) Mol. Gen. Genet. 178, 541-553]....

  11. Surveys of human enterotoxigenic Escherichia coli from three different geographical areas for possible colonization factors.

    OpenAIRE

    1991-01-01

    Enterotoxigenic Escherichia coli (ETEC) from Burma, central Africa (Rwanda and Zaire) and Peru, were screened by enzyme-linked immunoassays for the colonization factor antigens (CFAs) and putative colonization factors (PCFs): CFA/I, CFA/II, which consists of three coli surface-associated (CS) antigens, CS1, CS2 and CS3, CFA/III, CFA/IV (CS4, CS5, CS6), CS7, PCFO9, PCFO159. H4, PCFO166, and CS17. The highest proportion of ETEC with identifiable colonization factors (71%) were found in the stra...

  12. Sialyloligosaccharide chains of laminin as an extracellular matrix target for S fimbriae of Escherichia coli.

    OpenAIRE

    1993-01-01

    S fimbriae purified from recombinant Escherichia coli HB101(pANN801-13) bound strongly to extracellular matrices of cultured endothelial and epithelial cells; only poor binding was seen with the fimbriae purified from the sfaS mutant strain HB101(pANN801-1321). E. coli HB101(pANN801-13) adhered strongly to laminin immobilized on glass; no adhesion was seen to type I, III, IV, or V collagen. Strain HB101(pANN801-1321) failed to adhere to any of the target proteins. Adhesion to laminin of strai...

  13. Modeling surface growth of Escherichia coli on agar plates.

    Science.gov (United States)

    Fujikawa, Hiroshi; Morozumi, Satoshi

    2005-12-01

    Surface growth of Escherichia coli cells on a membrane filter placed on a nutrient agar plate under various conditions was studied with a mathematical model. The surface growth of bacterial cells showed a sigmoidal curve with time on a semilogarithmic plot. To describe it, a new logistic model that we presented earlier (H. Fujikawa et al., Food Microbiol. 21:501-509, 2004) was modified. Growth curves at various constant temperatures (10 to 34 degrees C) were successfully described with the modified model (model III). Model III gave better predictions of the rate constant of growth and the lag period than a modified Gompertz model and the Baranyi model. Using the parameter values of model III at the constant temperatures, surface growth at various temperatures was successfully predicted. Surface growth curves at various initial cell numbers were also sigmoidal and converged to the same maximum cell numbers at the stationary phase. Surface growth curves at various nutrient levels were also sigmoidal. The maximum cell number and the rate of growth were lower as the nutrient level decreased. The surface growth curve was the same as that in a liquid, except for the large curvature at the deceleration period. These curves were also well described with model III. The pattern of increase in the ATP content of cells grown on a surface was sigmoidal, similar to that for cell growth. We discovered several characteristics of the surface growth of bacterial cells under various growth conditions and examined the applicability of our model to describe these growth curves.

  14. Anomalia de Classe III

    OpenAIRE

    2014-01-01

    Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Medicina Dentária Introdução: A anomalia de classe III, é uma má oclusão que afeta os indivíduos psicologicamente, pois hoje em dia, a estética é socialmente valorizada. Deste modo, o diagnóstico deve ser executado precocemente para que os indivíduos portadores desta anomalia, possam ser acompanhados desde criança, pelos profissionais área da Medicina...

  15. Shiga Toxin Producing Escherichia coli.

    Science.gov (United States)

    Bryan, Allen; Youngster, Ilan; McAdam, Alexander J

    2015-06-01

    Shiga toxin-producing Escherichia coli (STEC) is among the common causes of foodborne gastroenteritis. STEC is defined by the production of specific toxins, but within this pathotype there is a diverse group of organisms. This diversity has important consequences for understanding the pathogenesis of the organism, as well as for selecting the optimum strategy for diagnostic testing in the clinical laboratory. This review includes discussions of the mechanisms of pathogenesis, the range of manifestations of infection, and the several different methods of laboratory detection of Shiga toxin-producing E coli.

  16. Experimental evolution of E. coli

    Science.gov (United States)

    Zhang, Mengshi

    The evolution from unicellular to multicellular behavior is an essential step in the history of life. Our aim is to investigate the emergence of collective behavior in the model organism Escherichia coli (E. coli) and its selection advantages, such as better utilization of public goods. Our preliminary results suggest that the evolution of collective behavior may be a natural response to stressed conditions. Mailing address: Room 306 Science Centre North Block, The Chinese University of Hong Kong, Shatin, N.T. Hong Kong SAR. Phone: +852-3943-6354. Fax: +852-2603-5204. E-mail: mengshi0928@gmail.com.

  17. Expression in E. coli systems

    DEFF Research Database (Denmark)

    Krogsdam, Anne-M; Kristiansen, Karsten; Nøhr, Jane

    2003-01-01

    intracellularly in soluble form. In E. coli, proteins containing disulfide bonds are best produced by secretion because the disulfide forming foldases reside in the periplasm. Likewise, a correct N-terminus is more likely to be obtained upon secretion. Moreover, potentially toxic proteins are more likely......Owing to cost advantage, speed of production, and often high product yield (up to 50% of total cell protein), expression in Escherichia coli is generally the first choice when attempting to express a recombinant protein. Expression systems exist to produce recombinant protein intracellularly...

  18. Pathogenomics of uropathogenic Escherichia coli

    Directory of Open Access Journals (Sweden)

    J Agarwal

    2012-01-01

    Full Text Available Subset of faecal E. coli that can enter, colonize urinary tract and cause infection are known as uropathogenic E. coli (UPEC. UPEC strains act as opportunistic intracellular pathogens taking advantage of host susceptibility using a diverse array of virulence factors. Presence of specific virulence associated genes on genomic/pathogenicity islands and involvement of horizontal gene transfer appears to account for evolution and diversity of UPEC. Recent success in large-scale genome sequencing and comparative genomics has helped in unravelling UPEC pathogenomics. Here we review recent findings regarding virulence characteristics of UPEC and mechanisms involved in pathogenesis of urinary tract infection.

  19. Stability constants of La(III), Pr(III), Nd(III), Sm(III), Gd(III), Tb(III) and Dy(III) complexes with N-(2-hydroxyphenyl) p-toluene sulphonamide

    Energy Technology Data Exchange (ETDEWEB)

    Chopra, S.C.; Mathur, K.C. (Jodhpur Univ. (India). Dept. of Chemistry)

    1981-01-01

    The dissociation constants of the ligand and stability constants of La(III), Pr(III), Nd(III), Sm(III), Gd(III), Tb(III) and Dy(III) complexes with N-(2-hydroxyphenyl) p-toluene sulphonamide have been determined potentiometrically using Calvin-Bjerrum pH titration technique at 30 +- 1deg C and ..mu.. = 0.1 M (NaClO/sub 4/) in aqueous medium. The stability constants of these metal complexes are found to follow the order Dy > Tb > Gd > Sm > Nd > Pr > La.

  20. Population III Hypernovae

    CERN Document Server

    Smidt, Joseph; Even, Wesley; Wiggins, Brandon; Johnson, Jarrett L; Fryer, Chris L

    2014-01-01

    Population III supernovae have been of growing interest of late for their potential to directly probe the properties of the first stars, particularly the most energetic events that are visible near the edge of the observable universe. But until now, hypernovae, the unusually energetic Type Ib/c supernovae that are sometimes associated with gamma-ray bursts, have been overlooked as cosmic lighthouses at the highest redshifts. In this, the latest of a series of studies on Population III supernovae, we present numerical simulations of 25 - 50 M$_{\\odot}$ hypernovae and their light curves done with the Los Alamos RAGE and SPECTRUM codes. We find that they will be visible at z = 10 - 15 to the James Webb Space Telescope (JWST) and z = 4 - 5 to the Wide-Field Infrared Survey Telescope (WFIRST), tracing star formation rates in the first galaxies and at the end of cosmological reionization. If, however, the hypernova crashes into a dense shell ejected by its progenitor, a superluminous event will occur that may be se...

  1. POPULATION III HYPERNOVAE

    Energy Technology Data Exchange (ETDEWEB)

    Smidt, Joseph; Whalen, Daniel J. [T-2, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Wiggins, Brandon K. [Department of Physics and Astronomy, Brigham Young University, Provo, UT 84602 (United States); Even, Wesley; Fryer, Chris L. [CCS-2, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Johnson, Jarrett L., E-mail: dwhalen1999@gmail.com [XTD-PRI, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States)

    2014-12-20

    Population III supernovae have been of growing interest of late for their potential to directly probe the properties of the first stars, particularly the most energetic events that are visible near the edge of the observable universe. Until now, hypernovae, the unusually energetic Type Ib/c supernovae that are sometimes associated with gamma-ray bursts, have been overlooked as cosmic beacons at the highest redshifts. In this, the latest of a series of studies on Population III supernovae, we present numerical simulations of 25-50 M {sub ☉} hypernovae and their light curves done with the Los Alamos RAGE and SPECTRUM codes. We find that they will be visible at z = 10-15 to the James Webb Space Telescope and z = 4-5 to the Wide-Field Infrared Survey Telescope, tracing star formation rates in the first galaxies and at the end of cosmological reionization. If, however, the hypernova crashes into a dense shell ejected by its progenitor, it is expected that a superluminous event will occur that may be seen at z ∼ 20 in the first generation of stars.

  2. Endothelin role in the orthostatic stress Papel da endotelina no estresse ortostático

    Directory of Open Access Journals (Sweden)

    Marli Cardoso Martins Pinge

    2008-10-01

    e, juntamente com componentes hormonais, amplia a capacidade de adaptação frente a mudanças posturais. Qualquer dificuldade nesse mecanismo compensatório que impeça seu funcionamento adequado pode resultar em falha da resposta com hipotensão, que, por sua vez, pode levar à hipoperfusão cerebral, hipóxia e perda de consciência. A manutenção da pressão arterial em níveis normais é importante para a homeostasia do meio interno. O barorreflexo desempenha papel fundamental no controle cardiovascular a curto-prazo na adaptação ao estresse ortostático, e prevendo excessivas flutuações da pressão arterial. Durante o estresse ortostático, também ocorrem mudanças neuroendócrinas como alteração nos níveis plasmáticos de endotelina. A endotelina, peptídeo composto de 21 aminoácidos, apresenta potente ação vasoconstritora. Tem sido sugerido um papel para a endotelina, já que está demonstrado que seus níveis no sangue estão aumentados em resposta a um estresse ortostático. Entretanto, embora os níveis de endotelina-1 aumentem em resposta ao estresse postural agudo, seu papel na homeostase cardiovascular e sua relação na liberação de outros hormônios ainda permanecem controversos e bastante desconhecidos em humanos in vivo.

  3. Paternidade: responsabilidade social do homem no papel de provedor Paternidad: responsabilidad social del hombre en el papel de proveedor Paternity: social responsibility of man's role as provider

    Directory of Open Access Journals (Sweden)

    Waglânia de Mendonça Faustino e Freitas

    2009-02-01

    Full Text Available OBJETIVO: Analisar os significados atribuídos à paternidade por homens que são pais. DELINEAMENTO DO ESTUDO: Estudo realizado em João Pessoa, PB, em 2003, desenvolvido sob abordagem qualitativa e enfoque teórico de gênero. Os participantes do estudo foram dez homens, cujos filhos eram atendidos no ambulatório de puericultura de um hospital escola. As informações analisadas foram obtidas por meio de entrevistas semi-estruturadas. Os depoimentos foram analisados pela técnica de análise crítica do discurso. ANÁLISE DOS DISCURSOS: Os sujeitos do estudo concebiam a paternidade como um novo encargo social, vinculando-a mais à provisão material da família do que ao espaço de envolvimento afetivo com o(a filho(a. Entretanto, os participantes experimentavam um processo de transição no qual o pai tradicional convivia entre aqueles cuja dimensão afetiva da paternidade apresentava-se como eixo central de preocupação do ser pai. CONCLUSÕES: O significado e o exercício concreto da paternidade situaram-se num campo de responsabilidades que predominantemente reproduzem o pai tradicional mas também recriam o papel de pai, com inclusão da dimensão afetiva.OBJETIVO: Analizar los significados atribuidos a la paternidad por hombres que son padres. DELINEAMIENTO DEL ESTUDIO: Estudio realizado en Joao Pessoa (Noreste de Brasil, en 2003, desarrollado bajo el abordaje cualitativo y enfoque teórico del género. Los participantes del estudio fueron diez hombres, cuyos hijos eran atendidos en el ambulatorio de puericultura de un hospital escuela. Las informaciones analizadas fueron obtenidas por medio de entrevistas semi-estructuradas. Los relatos fueron analizados por la técnica de análisis crítica del discurso. ANÁLISIS DE LOS DISCURSOS: Los sujetos de estudio concebían la paternidad como un nuevo encargo social, vinculándola más a la provisión material de la familia que al espacio de envolvimiento afectivo con él(la hijo(a. As

  4. Infektionen mit darmpathogenen Escherichia coli.

    NARCIS (Netherlands)

    Friedrich, Alexander; Stein, Jürgen; Dignass, Axel

    2001-01-01

    E. coli ist ein wesentlicher Bestandteil der physiologischen Darmflora des Menschen. Die üblicherweise im Darm vorkommenden Kolibakterien sind apathogen und für den Menschen eher nützlich (Sonnenborn u. Greinwald 1990). Allerdings kennen wir bei dieser Bakterienspezies auch ein breites Spektrum von

  5. Expression in E. coli systems

    DEFF Research Database (Denmark)

    Krogsdam, Anne-M; Kristiansen, Karsten; Nøhr, Jane

    2003-01-01

    intracellularly in soluble form. In E. coli, proteins containing disulfide bonds are best produced by secretion because the disulfide forming foldases reside in the periplasm. Likewise, a correct N-terminus is more likely to be obtained upon secretion. Moreover, potentially toxic proteins are more likely...

  6. Papel de las mitocondrias y el estrés oxidativo en el proceso inflamatorio renal

    Directory of Open Access Journals (Sweden)

    Walter Manucha

    2014-06-01

    Full Text Available La muerte celular programada y la fibrosis renal son procesos inherentes a la enfermedad renal crónica y, en tal sentido, ha sido recientemente descripta una clara desregulación de la maquinaria respiratoria mitocondrial en pacientes con enfermedad renal crónica asociada con un aumento del estrés oxidativo. Las células tubulares lesionadas vinculadas a los macrófagos intersticiales y miofibroblastos producen citoquinas y factores de crecimiento que promueven un estado inflamatorio, inducen la apoptosis de las células tubulares y facilitan la acumulación de matriz extracelular. La angiotensina II desempeña un papel central en la fibrogénesis renal y conduce a una rápida progresión de la enfermedad renal crónica. Los niveles crecientes de la angiotensina II inducen citoquinas pro-inflamatorias, la activación de NF-kB, moléculas de adhesión, quimiocinas, factores de crecimiento y estrés oxidativo. Toda la evidencia actual sugiere que la angiotensina II aumenta el estrés oxidativo mitocondrial, regula la inducción de apoptosis y condiciona al estado inflamatorio. Por lo tanto, existiría un papel determinante de las mitocondrias y el estrés oxidativo en el proceso inflamatorio renal. Finalmente, esta revisión resume nuestro actual conocimiento acerca de los posibles mecanismos que contribuirían con la apoptosis modulada por la inflamación y/o el estrés oxidativo durante la enfermedad renal crónica. Además, se propone un nuevo concepto de herramientas anti-inflamatorias que regulan el estrés oxidativo mitocondrial lo cual afectaría directamente al proceso inflamatorio y la apoptosis. Esta idea podría tener consecuencias atractivas sobre el tratamiento de patologías inflamatorias renales y de otras afines.

  7. Papel de las células nkt invariantes en la respuesta inmune anti-viral.

    Directory of Open Access Journals (Sweden)

    Alejandro Román

    2009-11-01

    Full Text Available Las células T asesinas naturales con receptor de células T invariante y restringidas por la molécula CD1d (iNKT son un subgrupo de linfocitos con potente actividad inmunorreguladora; su respuesta casi inmediata y la capacidad de producir citoquinas tanto Th1 como Th2 son factores determinantes en el desarrollo de la respuesta inmune innata y adaptativa. El papel fisiológico de las células iNKT se ha documentado ampliamente en la respuesta anti-tumoral, el desarrollo de la tolerancia en los órganos inmunoprivilegiados y el control de las reacciones autoinmunes. A pesar de la demostrada potencia inmunomoduladora de las células iNKT, hasta el momento se conoce poco de su acción en la respuesta inmune anti-infecciosa, en particular en el ser humano y contra los virus patógenos. Este artículo sintetiza los resultados de una búsqueda en las principales bases de datos biomédicas (Pubmed, Medline y OVID, e incluye los estudios realizados para caracterizar estas células y evaluar su papel en la interacción del hospedero con los virus. Las células iNKT participan en la respuesta inmune antiviral, aunque de una manera diferente según el tipo de virus; incluso, podrían estar comprometidas en los daños mediados por mecanismos inmunes. En el ser humano, las células iNKT son aparentemente esenciales en la respuesta inmune contra el virus Varicela Zoster, mientras que todavía hay controversia sobre su función en el control de otros virus. Los modelos animales han aportado las primeras evidencias sobre el potencial de la manipulación terapéutica específica de este subgrupo de linfocitos.

  8. Papel de las células nkt invariantes en la respuesta inmune anti-viral

    Directory of Open Access Journals (Sweden)

    Alejandro Román

    2006-06-01

    Full Text Available Las células T asesinas naturales con receptor de células T invariante y restringidas por la molécula CD1d (iNKT son un subgrupo de linfocitos con potente actividad inmunorreguladora; su respuesta casi inmediata y la capacidad de producir citoquinas tanto Th1 como Th2 son factores determinantes en el desarrollo de la respuesta inmune innata y adaptativa. El papel fisiológico de las células iNKT se ha documentado ampliamente en la respuesta anti-tumoral, el desarrollo de la tolerancia en los órganos inmunoprivilegiados y el control de las reacciones autoinmunes. A pesar de la demostrada potencia inmunomoduladora de las células iNKT, hasta el momento se conoce poco de su acción en la respuesta inmune anti-infecciosa, en particular en el ser humano y contra los virus patógenos. Este artículo sintetiza los resultados de una búsqueda en las principales bases de datos biomédicas (Pubmed, Medline y OVID, e incluye los estudios realizados para caracterizar estas células y evaluar su papel en la interacción del hospedero con los virus. Las células iNKT participan en la respuesta inmune antiviral, aunque de una manera diferente según el tipo de virus; incluso, podrían estar comprometidas en los daños mediados por mecanismos inmunes. En el ser humano, las células iNKT son aparentemente esenciales en la respuesta inmune contra el virus Varicela Zoster, mientras que todavía hay controversia sobre su función en el control de otros virus. Los modelos animales han aportado las primeras evidencias sobre el potencial de la manipulación terapéutica específica de este subgrupo de linfocitos.

  9. Educación física emocional en secundaria. El papel de la sociomotricidad

    Directory of Open Access Journals (Sweden)

    Conxita Duran Delgado

    2014-09-01

    Full Text Available La educación física puede ejercer un papel destacado en la educación de competencias emocionales, las cuales repercuten direc-tamente en el rendimiento académico del alumnado y en la mejora de su bienestar subjetivo. En este trabajo se examinó la vivencia emocional de adolescentes en diferentes clases de juegos deportivos, con presencia y ausencia de competición. Correspondió a un diseño cuasi-experimental de medidas repetidas. Participaron 220 estudiantes de ESO y bachillerato de 5 centros educativos de Cataluña y Valencia. Se realizaron 4 sesiones basadas en diferentes familias de juegos deportivos. A través de la escala validada Games and emotions Scale (GES los alumnos identificaron la vivencia emocional originada en cada juego. El análisis de los datos se realizó mediante ecuaciones de estimación generalizadas (GEE. El modelo consideró los factores intra-participantes: 1 dominio de acción motriz; 2 competición y 3 tipo de emoción y los factores entre-participantes, que fueron: 1 género; y 2 historial deportivo. De los resultados se obtienen tres hallazgos principales: a el papel destacado de los juegos sociomotores para promover emociones positivas intensas; b la presencia de adversarios (en juegos de oposición y de cooperación-oposición aumenta la intensidad de las emociones negativas; c la introducción de la competición incrementa la intensidad de las emociones positivas en los juegos psicomotores y los valores de las emociones negativas en los juegos sociomotores. Este estudio aporta argumentos científicos y recomendaciones prácticas para promover una educación física emocional orientada hacia el bienestar subjetivo y a la adquisición de estilos de vida saludables en adolescentes.

  10. El movimiento de los sin-papeles como sujeto de juridicidad

    Directory of Open Access Journals (Sweden)

    Barbero, Iker

    2013-04-01

    Full Text Available From a traditional perspective of Law, the production of legal rules is limited to State powers (legislative, executive and judiciary as well as to some social agents legitimated through mechanisms of social participation foreseen in the legal system. The regulatory capacity of agents not recognized as subjects of legality can only be investigated from a sociological perspective of alternativity of Law. This paper draws attention on acts of migrants, especially those irregular immigrants or “sin-papeles” as subjects of legality. Starting with an analysis of the social, legal and political context of the sit-ins and other acts of mobilization that took place in 2001, we will investigate the capacity of the sin-papeles movement in Barcelona to influence legal production either by creating, modifying or altering the policy agenda on migration.Desde un punto de vista formalista del Derecho, la producción de normas jurídicas está limitada a los poderes del Estado (legislativo, ejecutivo y judicial así como a algunos agentes sociales legitimados por mecanismos de participación previstos en el ordenamiento jurídico. La capacidad normativa de agentes no reconocidos como sujetos de juridicidad únicamente puede ser investigada desde una perspectiva sociológica de la alternatividad del Derecho. Este texto presta atención a los actos de migrantes como sujetos de juridicidad, y especialmente a aquellos construidos social y normativamente como irregulares o autodefinidos como “sin-papeles”. A partir de la descripción de circunstancias sociales, jurídicas y políticas en torno a los encierros y otros actos de movilización que tuvieron lugar en el año 2001, se pretende analizar cuál fue la capacidad de los sin-papeles para incidir en la producción legal ya sea creando, modificando o alterando la agenda normativa en materia migratoria.

  11. Aplicación de la celulosa bacteriana a la restauración del patrimonio bibliográfico y documental en papel

    OpenAIRE

    Santos de Dios, Sara M.

    2015-01-01

    La preservación del patrimonio bibliográfico y documental en papel es uno de los mayores retos a los que se enfrentan bibliotecas y archivos de todo el mundo. La búsqueda de soluciones al problema del papel degradado ha sido abordada históricamente desde dos líneas de trabajo predominantes: la conservación de estos documentos mediante la neutralización de los ácidos presentes en ellos con agentes alcalinos, y su restauración mediante el método de laminación fundamentalmente con papel de orige...

  12. Efecto del tipo tinta y de las propiedades del papel estucado de alta gama en la calidad de impresión

    OpenAIRE

    Quintana, Ester; Gomez Hernandez, Nuria; Villar, Juan Carlos; J. Miranda; Quiles, R.

    2010-01-01

    En los últimos años, el gran desarrollo tecnológico experimentado por el sector de la impresión offset, unido a que cada vez los costes de producción son más ajustados, ha producido un aumento de la exigencia de la calidad del papel. La calidad de impresión de un papel estucado se evalúa mediante la densidad, las coordenadas colorimétricas y el brillo de la impresión. El objetivo del estudio fue determinar la influencia de las propiedades del papel estucado y de la tinta sobre las propie...

  13. O papel de espelho em um Centro de Atenção Psicossocial El papel del espejo en un Centro de Atención Psicosocial The role of the mirror at a Psychosocial Care Center

    Directory of Open Access Journals (Sweden)

    Cristiane Bach Franchini

    2008-09-01

    Full Text Available Este trabalho pretende refletir sobre o papel de espelho no tratamento oferecido em um centro de atenção psicossocial, o CAPS-T, utilizando-se o referencial psicanalítico com abordagem Winnicottiana. A mãe, que funciona como um espelho, reflete ao bebê, em seu olhar, algo que se refere a ele, e ser visto é uma das bases para o sentimento de existir, de forma que o "si-mesmo" verdadeiro, reconhecido, pode constituir-se. Quando o papel de espelho está ausente, o bebê não vê a si, o que pode levá-lo a uma existência reativa. No CAPS, com uma alta incidência de pacientes psicóticos, torna-se fundamental o oferecimento de uma provisão ambiental adequada, o que inclui o papel de espelho, facilitando o processo de amadurecimento. A equipe deve ser um espelho único e atuar no ambiente social do paciente, possibilitando o surgimento do papel de espelho nas pessoas e a construção de um ambiente mais adequado.Este artículo pretende hacer una reflexión sobre el papel del espejo en un Centro de Atención Psicosocial, Caps - T, utilizando el referencial Psicoanalitico de abordaje Winnicottiano. La madre que funciona como un espejo refleja al bebé con su mirada algo que a él se refiere. Ser visto es una de las bases para el sentimiento de existir, de manera que el verdadero si mismo, reconocido, puede constituirse. Cuando el papel de espejo esta ausente, el bebé no se puede ver a si mismo, lo que puede llevarlo a una existencia reactiva. En el CAPS es fundamental que se le ofrezca una provisión ambiental adecuada, lo que incluye el papel del espejo, facilitando el proceso de maduración. El equipo debe ser un único espejo y actuar en el ambiente social del paciente, propiciando el surgimiento del papel de espejo en las personas y la construcción de un ambiente más adecuado.The role of the mirror in the treatment at a Psychosocial Care Center called CAPS-T, using a Winnicottian psychoanalytical framework is provided. The mother, as a

  14. Fast ejendom III

    DEFF Research Database (Denmark)

    Munk-Hansen, Carsten

    Bogen er det tredje bind af tre planlagte bind om fast ejendom: I Overdragelsen, II Bolighandlen og III Ejerbeføjelsen. Fremstillingens giver et grundigt overblik over centrale områder af en omfattende regulering af fast ejendom, med angivelse af litteratur, hvor læseren kan søge yderligere...... oplysning. En ejer af fast ejendom er på særdeles mange områder begrænset i sin råden sammenlignet med ejeren af et formuegode i almindelighed. Fremstillingen tager udgangspunkt i ejerens perspektiv (fremfor samfundets eller myndighedernes). Både den privatretlige og offentligretlige regulering behandles......, eksempelvis ejendomsdannelsen, servitutter, naboretten, hævd, zoneinddelingen, den fysiske planlægning, beskyttelse af natur, beskyttelse af kultur, forurening fra fast ejendom, erstatning for forurening, jordforurening, ekspropriation, byggeri og adgang til fast ejendom....

  15. Ammonium diphosphitoindate(III

    Directory of Open Access Journals (Sweden)

    Farida Hamchaoui

    2013-04-01

    Full Text Available The crystal structure of the title compound, NH4[In(HPO32], is built up from InIII cations (site symmetry 3m. adopting an octahedral environment and two different phosphite anions (each with site symmetry 3m. exhibiting a triangular–pyramidal geometry. Each InO6 octahedron shares its six apices with hydrogen phosphite groups. Reciprocally, each HPO3 group shares all its O atoms with three different metal cations, leading to [In(HPO32]− layers which propagate in the ab plane. The ammonium cation likewise has site symmetry 3m.. In the structure, the cations are located between the [In(HPO32]− layers of the host framework. The sheets are held together by hydrogen bonds formed between the NH4+ cations and the O atoms of the framework.

  16. Oxymatrinium tetrachloridoferrate(III

    Directory of Open Access Journals (Sweden)

    Xiong He

    2012-02-01

    Full Text Available The asymmetric unit of the title compound, (C15H25N2O2[FeCl4], contains a tetrachloridoferrate(III anion and a oxymatrinium cation [oxymatrine is (4R,7aS,13aR,13bR,13cS-dodecahydro-1H,5H,10H-dipyrido[2,1-f:3′,2′,1′-ij][1,6]naphthyridin-10-one 4-oxide]. The conformation of oxymatrine is similar to that of matrine with one ring having a half-chair conformation, while the others have chair conformations. Chiral chains of cations along the c axis are formed by O—H...O hydrogen bonds.

  17. III.— Documents

    OpenAIRE

    2014-01-01

    III/1 inventaire de l’orangerie du château d’Ivry, 1770 • Inventaire après décès du marquis de Béringhen (extrait), AN M.C. LXXXVIII, 723, 28 février 1770 « [f° 113 v°] Dudit jour samedi seize du présent mois de juin huit heures du matin à même requête qualité et preuve que dessus, ainsi que le tout a été ci-devant établi, va être par les conseillers du roi notaires au Châtelet de Paris [f° 114 r°] soussignés procédé à la continuation du présent inventaire de la manière et ainsi qu’il suit. S...

  18. Pseudo Class III malocclusion.

    Science.gov (United States)

    Al-Hummayani, Fadia M

    2016-04-01

    The treatment of deep anterior crossbite is technically challenging due to the difficulty of placing traditional brackets with fixed appliances. This case report represents a none traditional treatment modality to treat deep anterior crossbite in an adult pseudo class III malocclusion complicated by severely retruded, supraerupted upper and lower incisors. Treatment was carried out in 2 phases. Phase I treatment was performed by removable appliance "modified Hawley appliance with inverted labial bow," some modifications were carried out to it to suit the presented case. Positive overbite and overjet was accomplished in one month, in this phase with minimal forces exerted on the lower incisors. Whereas, phase II treatment was performed with fixed appliances (braces) to align teeth and have proper over bite and overjet and to close posterior open bite, this phase was accomplished within 11 month.

  19. Host Response to Porcine Strains of Escherichia coli in a Novel Pyelonephritis Model

    DEFF Research Database (Denmark)

    Isling, L. K.; Aalbæk, B.; Birck, M. M.;

    2011-01-01

    . coli strain LK67 (P fimbriae PapGI), LK76 (type 1 fimbriae) or LK82 (type 1 fimbriae and P fimbriae PapGII/III), respectively. The contralateral kidneys were inoculated with saline and served as controls. Pigs were killed 6h post-inoculation (hpi). Differential leucocyte counts, serum biochemical...... kidneys. Gross and microscopical lesions of acute pyelonephritis were demonstrated in all but one kidney inoculated with E. coli, but in none of the control kidneys. Renal parenchymal infiltration with both neutrophils and mononuclear cells, primarily CD3+ T lymphocytes, was observed at 6hpi. Most T...... lymphocytes were CD8+. Pigs in group C had the highest mean pathology scores. Neutrophils were the dominant renal leucocyte in this group, while the number of mononuclear cells was at least equal to the number of neutrophils in the lesions of pigs from groups A and B. Kidneys with a high number of E. coli had...

  20. Semiconducting III-V compounds

    CERN Document Server

    Hilsum, C; Henisch, Heinz R

    1961-01-01

    Semiconducting III-V Compounds deals with the properties of III-V compounds as a family of semiconducting crystals and relates these compounds to the monatomic semiconductors silicon and germanium. Emphasis is placed on physical processes that are peculiar to III-V compounds, particularly those that combine boron, aluminum, gallium, and indium with phosphorus, arsenic, and antimony (for example, indium antimonide, indium arsenide, gallium antimonide, and gallium arsenide).Comprised of eight chapters, this book begins with an assessment of the crystal structure and binding of III-V compounds, f

  1. El papel del desarrollo financiero como fuente del crecimiento económico

    Directory of Open Access Journals (Sweden)

    José Luis Hernández Mota

    2015-11-01

    Full Text Available Recientemente ha resurgido el debate por el papel del sistema financiero en el crecimiento económico; sin embargo, en la mayor parte de la literatura se tiene como finalidad medir el impacto del primero sobre el segundo, a través de relaciones econométricas. Por ello, el presente trabajo contribuye a la literatura retomando el análisis del modelo canónico de crecimiento económico, donde se introduce la variable financiera como factor que puede provocar una influencia permanente sobre la tasa de crecimiento económico por medio de su grado de complementariedad con la acumulación de capital y la productividad, y no únicamente como un factor explicativo de ella. Así, se manifiesta la importancia de los efectos reales que provoca el desarrollo del sistema financiero en los factores del crecimiento económico; ello, desde un marco macroeconómico aplicable tanto para las economías desarrolladas como para las emergentes.

  2. El aprendizaje para el cambio. Papel de la educación

    Directory of Open Access Journals (Sweden)

    Mario Rodríguez Mena García

    2003-01-01

    Full Text Available Este artículo tiene la intención de provocar la reflexión acerca del papel que ha de jugar la educación de hoy en promover verdaderos aprendizajes, que sirvan a las personas para enfrentar las exigencias de cambio, en todos los órdenes, que la nueva sociedad impone. Se presenta, someramente, el programa de “Formación de Aprendices Autorregulados en Comunidades de Aprendizaje”, que los autores llevan a cabo como parte de su labor investigadora en el Centro de Investigaciones Psicológicas y Sociológicas del Ministerio de Ciencia, Tecnología y Medio Ambiente de Cuba y como respuesta a esta demanda social; y sus experiencias en la formación de educadores mexicanos del estado de Morelos que hoy tienen ya la posibilidad de implementar en sus aulas el modelo de formación que propugna dicho programa.

  3. O papel dos esquemas na memória de idosas saudáveis

    Directory of Open Access Journals (Sweden)

    Claudimara Chisté Santos

    2012-06-01

    Full Text Available A memória é tradicionalmente estudada pela psicologia cognitiva e pela neurociência. A presente pesquisa se baseia em uma obra de Piaget dedicada ao estudo desse fenômeno com base em paradigmas construtivistas e replica um de seus experimentos, fazendo uma adaptação dos níveis de análise. O objetivo que orientou esse estudo foi identificar o papel dos esquemas em um experimento de memória no sentido amplo, com base na teoria de Piaget. As seis participantes que contribuíram com o estudo estavam, na época da pesquisa, com idades entre 61 e 67 anos e tinham, no mínimo, o Ensino Médio. Os resultados indicam que: (1 a adaptação dos níveis de análise atendeu à classificação da memória no experimento; (2 foi possível identificar, por meio do método clínico, a participação dos esquemas construídos ao longo da vida como recurso para recuperação da informação, o que pode contribuir para futuros estudos sobre intervenção na memória de idosos.

  4. Aplicaciones biológicas de las fitasas: papel en los fertilizantes microbianos

    Directory of Open Access Journals (Sweden)

    María Teresa Fernández

    2006-01-01

    Full Text Available La mayoría de los suelos contienen grandes cantidades de P total que aparece tanto en fracciones orgánicas como inorgánicas y debido a la fertilización con fósforo. El mayor componente de fósforo orgánico en el suelo es el fitato (ácido fítico y es degradado por una clase de enzimas colectivamente denominadas fitasas. Los microorganismos juegan un importante papel en procesos que afectan la transformación del ácido fítico en el suelo y su disponibilidad para las plantas. Debido a la inhabilidad de las plantas para utilizar el fósforo contenido en el ácido fítico, la ingeniería genética ofrece oportunidades de manipulación de los genes de fitasas para lograr microorganismos más eficientes que ayuden a incrementar la movilización y disponibilidad del fósforo hacia las plantas mejorando la nutrición de las mismas.

  5. O papel da televisão na construção legislativa brasileira

    Directory of Open Access Journals (Sweden)

    Renata Caleffi

    2014-07-01

    Full Text Available RESUMO O processo legislativo brasileiro é colocado, algumas vezes, em questionamento, ora por ser apressado, ora por ser lento. Mais profundo que apenas o tempo em que um projeto de lei é discutido no Congresso Nacional, alguns pesquisadores conferem ainda aos veículos de comunicação uma parcela da culpa por algumas leis estarem sendo aprovadas na atualidade. O presente artigo busca encontrar alternativas para explicar a aprovação de leis no Brasil, destacando o papel da televisão como um ator social e não de uma autora legislativa. Como a televisão no Brasil é o meio mais consumido para obter informação, e o Jornal Nacional o programa de maior audiência, o telejornal é então, o objeto de pesquisa. Por meio da teoria dos múltiplos fluxos de Kingdon (2003, que dão origem às janelas de oportunidade, foi observada uma nova visão de como a construção legislativa pode ser uma estratégia política utilizada com frequência pelo Congresso Nacional e também, uma oportunidade do telejornal para pautar assuntos de caráter legislativo dentro da sociedade. Para exemplificar o processo legislativo, foram utilizadas as Leis 12.760/2012 (Lei Seca e a 12.737/2012 (Lei Carolina Dieckmann. As alternativas apresentadas neste artigo auxiliam as pesquisas interessadas em debater o papel da mídia e as relações de poder existentes entre os atores políticos nacionais, sejam em situação eletiva ou não, destacando as relações entre televisão e o Congresso e valorizando as estratégias comunicacionais dentro da política brasileira. Palavras-chave: Televisão.  Janelas da Oportunidade. Leis.  Estratégias. Política.   ABSTRACT Sometimes the Brazilian legislative process is questioned, either for being considered rushed or for being slow. Deeper than only the period in which a bill is discussed in the Congress, some researchers also blame the means of communication for some laws that have been currently adopted. This article seeks to

  6. O papel mediador entre confiança e desempenho organizacional

    Directory of Open Access Journals (Sweden)

    Marco Tulio Fundão Zanini

    2014-03-01

    Full Text Available Neste artigo, busca-se contribuir para as discussões sobre a relação entre confiança e desempenho organizacional numa perspectiva econômica. Examinou-se o papel mediador entre confiança e desempenho organizacional a partir da análise da confiança como um elemento de coordenação informal dentro das organizações. Observou-se que a confiança interpessoal é um elemento central para a melhor compreensão dos contratos relacionais, como os contratos de trabalho, e que investimentos na criação e na manutenção de ambientes de trabalho baseados em confiança devem observar uma racionalidade econômica. Por meio de revisão na literatura para buscar compreender a necessidade e os benefícios de contextos sociais de alta e baixa confiança, concluiu-se que confiança é um elemento essencial para a execução de específicas tarefas organizacionais e observou-se sua relação mediadora com o desempenho.

  7. EL PAPEL DE LAS COMPETENCIAS INDIVIDUALES Y COLECTIVAS EN LOS SISTEMAS DE ACCIÓN

    Directory of Open Access Journals (Sweden)

    Juan B. Climént Bonilla

    2009-01-01

    Full Text Available El principal propósito de este artículo, producto de un extensa revisión de términos y conceptos relacionados con educación y capacitación basadas en competencias, es aclarar el papel de las competencias individuales y colectivas en el contexto de los sistemas de acción, donde ambos tipos se complementan -como instrumentos de la formación de individuos y grupos- en la solución de problemas y necesidades que afectan a las personas en distintos espacios de participación e intervención social. Con el mismo propósito, se examinan varias de las diferencias y puntos de encuentro, entre competencias individuales y colectivas, especialmente en lo que concierne a los componentes primarios y las funciones sustantivas de los sistemas de acción. Finalmente, se advierte sobre los efectos adversos que pueden tener las competencias falsas o desnaturalizadas, en el funcionamiento y la sustentabilidad de dichos sistemas.

  8. O papel de marcadores moleculares na genética forense

    Directory of Open Access Journals (Sweden)

    Daniele Decanine

    2016-07-01

    Full Text Available O objetivo desse trabalho foi apresentar uma revisão bibliográfica sobre as tecnologias utilizadas na Genética Forense, enfatizando o uso de marcadores moleculares para a identificação humana. Apresento aqui alguns exemplos do potencial da Biologia Molecular para auxiliar na investigação criminal, bem como na definição de parentesco (maternidade e paternidade. A utilização desses marcadores é atualmente a peça fundamental para os testes de DNA forense. Estes sistemas são, na sua maioria, baseados na análise de painéis de sequências microssatélites específicas (STRs. Foi possível discorrer sobre o uso forense do DNA, sobre a presença de regiões hipervariáveis no material genético, o papel de marcadores moleculares, bem como abordar técnicas de análise de DNA e suas aplicações. A busca por novas metodologias se faz importante para reduzir os custos e impulsionar uma nova cultura genética na Ciência Forense, as quais terão impacto no futuro do DNA forense com a expansão da Biologia Molecular.

  9. EL PAPEL DEL CAPITAL HUMANO EN EL SECTOR TURÍSTICO: ALGUNAS REFLEXIONES Y PROPUESTAS

    Directory of Open Access Journals (Sweden)

    Adelaida Lillo Bañuls

    2009-01-01

    Full Text Available En este trabajo se reflexiona sobre el papel del capital humano como factor estratégico para el logro de objetivos de competitividad y diferenciación en las organizaciones turísticas. Asimismo, se plantea la necesidad de establecer distintas escalas de alcance en la formación turística para poder determinar las posibles líneas de actuación en las políticas educativas y en el fomento de la investigación en turismo. Así, con el fin de incrementar el nivel de educación y la preparación de los trabajadores del sector, se plantean propuestas para tratar de acercar la formación turística a las necesidades reales de su tejido productivo para mejorar tanto la calidad de su capital humano como la valoración del mismo y su rendimiento educativo. Es este un factor indispensable para permitir el desarrollo de un sector sólido y sostenible, capaz de competir en un entorno global y ante situaciones cambiantes.

  10. Papel de la IL-6 y TNF-alfa en la enfermedad periodontal

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    A. Carrillo de Albornoz Sainz

    Full Text Available La periodontitis se inicia por una serie de patógenos que inducen una cascada inflamatoria que estimula la destrucción de los tejidos mediada por el huésped. Están implicados un gran número de mediadores inflamatorios, entre los que destacan IL-6 y TNF-alfa. La IL-6 es una citoquina pleiotrópica de compleja actividad biológica, cuya función principal vinculada a la periodontitis es la inducción de la reabsorción ósea. TNF-alfa es una citoquina proinflamatoria cuya función más destacada es el reclutamiento y estimulación de neutrófilos y monocitos. Su papel en la periodontitis es diverso, ya que promueve tanto la inflamación, como la pérdida ósea y la destrucción del tejido conectivo, a la vez que limita la capacidad de reparación del periodonto.

  11. Papel de la proteína C reactiva en las enfermedades cardiovasculares

    Directory of Open Access Journals (Sweden)

    Fernando Manzur, MD., FACC

    2011-08-01

    Full Text Available La proteína C-reactiva (PCR, un marcador sensible de inflamación, es un predictor independiente de enfermedad cardiovascular futura (ECV, que es una de las principales causas de muerte en todo el mundo. El papel de la inflamación en esta entidad ha sido bien documentado en la última década, y se ha demostrado inflamación en todas las fases de la aterosclerosis, desde el inicio y el crecimiento, hasta la ruptura de la placa. La PCR es una proteína de fase aguda, altamente sensible como marcador de inflamación general. En estudios experimentales, se ha determinado la presencia de PCR en arterias que presentan lesiones ateroscleróticas. También se ha demostrado que, en forma directa, la PCR induce la producción de otras células inflamatorias y que disminuye la expresión de la óxido nítrico sintetasa. Es decir, desde el punto de vista biológico, la PCR participa en el proceso aterogénico. En adultos, la PCR, detectada con técnicas ultrasensibles (PCRus, se asocia con los factores de riesgo tradicionales y su concentración predice eventos cardiovasculares.

  12. CONSIDERACIONES MICROECONÓMICAS SOBRE EL PAPEL DEL ESTADO EN LA ECONOMÍA DE MERCADO

    Directory of Open Access Journals (Sweden)

    Wilmar de Jesús Briceño Rondón

    2010-11-01

    investigación económica. El objetivo de la presente investigación es presentar un ensayo desde el punto de vista microeconómico sobre el papel que tiene el estado en una economía de mercado. Se realiza una investigación enfocada en el paradigma fenomenológico interpretativo, cualitativo y documental, cuyo principal objetivo es la descripción de las cualidades que caracterizan el fenómeno objeto de estudio. A manera de conclusión: El mercado y el estado son categorías interrelacionadas que se requieren mutuamente, independientemente del sistema económico prevaleciente. El estado requiere al mercado para garantizar la circulación y la acumulación; esencial para el funcionamiento del estado. El mercado requiere al estado, por ser éste último el facilitador y regulador de primera y última instancia, tanto en períodos de bonanza como de crisis. En suma, una sociedad eficiente y humana requiere de las dos mitades del sistema económico: el Estado y el Mercado.

  13. Los desastres de origen natural en México: el papel del FONDEN

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    José Manuel Rodríguez Esteves

    2004-01-01

    Full Text Available Por su ubicaciÛn geogr·fica, MÈxico es escenario de la manifestaciÛn de va- rios tipos de fenÛmenos naturales. Los huracanes y los sismos son quiz·s las principales fuentes de amenaza que enfrenta el territorio nacional y cuya intensidad puede ocasionar de- sastres. El impacto que los desastres de origen natural pueden llegar a cau- sar debe considerarse en la planea- ciÛn de los centros de poblaciÛn. El presente trabajo aborda las con- secuencias que han tenido los desas- tres de origen natural en MÈxico en los ̇ltimos aÒos. Ante la nevitabilidad de la manifestaciÛn de estos fenÛme- nos, se analiza el papel que ha jugado el Fondo Nacional de Desastres Natu- rales ( FONDEN como instrumento financiero para atender a la poblaciÛn afec- tada por los mismos.

  14. O PAPEL DA EDUCAÇÃO ESCOLAR NO DESENVOLVIMENTO DA PERSONALIDADE DO ADOLESCENTE

    Directory of Open Access Journals (Sweden)

    Ricardo Eleutério dos Anjos

    2014-05-01

    Full Text Available A partir dos pressupostos da psicologia histórico-cultural e da pedagogia histórico-crítica, este artigo procurou analisar teoricamente o papel da educação escolar no desenvolvimento da personalidade do adolescente. Foram pesquisadas não somente as obras de Vygotski, como também de Leontiev, Luria, Elkonin, entre outros autores que contribuíram e contribuem para a formação do conjunto de trabalhos elaborados pela psicologia histórico-cultural. A pedagogia histórico-crítica, a partir das obras de Dermeval Saviani e Newton Duarte, foi utilizada como mediação entre a psicologia histórico-cultural e a educação escolar, por entender que esta teoria pedagógica é compatível com os pressupostos da “Escola de Vygotski”. Este trabalho apresenta a concepção da psicologia histórico-cultural sobre a personalidade do adolescente como superação das concepções psicológicas naturalizantes e idealistas, explicitando, ainda, a relevância da transmissão dos conteúdos escolares para o desenvolvimento da personalidade nessa idade de transição.http://dx.doi.org/10.14572/nuances.v25i1.2941

  15. El papel de las remesas económicas y sociales en las familias transnacionales colombianas

    Directory of Open Access Journals (Sweden)

    Ana María Rivas

    2011-01-01

    Full Text Available Este artículo recoge parte de los resultados de una investigación sobre el papel de las remesas económicas y sociales en las familias migrantes transnacionales colombianas del Área Metropolitana Valle de Aburrá y Área Metropolitana Centro Occidente, con algunos de sus miembros en la comunidad de Madrid, en España. En la primera parte se desarrollan los conceptos clave que han sido utilizados en el análisis desde el paradigma transnacional. A continuación se presentan los objetivos y la metodología y, por último, se exponen algunos resultados en torno de la influencia de las remesas económicas y sociales en los procesos de transformación y permanencia de las relaciones y prácticas tradicionales de género, así como en las relaciones intergeneracionales, teniendo en cuenta: 1 el tipo de familia al que pertenecían las/os entrevistadas/os antes de la partida de la/el migrante, y 2 la dimensión temporal dentro del proceso migratorio.

  16. O papel do controle interno na administração pública

    Directory of Open Access Journals (Sweden)

    Pedro Gabril Kenne da Silva

    2002-01-01

    Full Text Available Neste trabalho estuda-se o controle enquanto função da Ciência da Administração e sua utilização como principal objeto da Auditoria Interna, centrando atenção à evolução dos controles internos na Administração Pública. Abordam-se a forma de organização, papel e importância do controle interno, assim como as dificuldades para o estabelecimento de controles eficazes no setor público, em função de suas peculiaridades. Ao final, tomando-se por base o sistema de controle interno do Estado do Rio Grande do Sul, propõem-se medidas para que se estabeleça, de fato, uma integração entre o órgão central do sistema de controle interno e as unidades ou funções de controle da Administração Pública.

  17. Papel de los macrófagos en la patogénesis de la arteriosclerosis

    Directory of Open Access Journals (Sweden)

    Quintana Varón María del Pilar

    2007-12-01

    Full Text Available La arteriosclerosis es una enfermedad inflamatoria que es responsable del 50% de las muertes del mundo
    occidental por desencadenar complicaciones coronarias graves. Se produce por la acumulación excesiva de
    colesterol proveniente del torrente sanguíneo en la íntima arterial de arterias medias y grandes. Los monocitos circulantes se adhieren al endotelio activado y se diferencian en macrófagos al interior de la íntima
    arterial, donde se desempeñan como recolectores del exceso de colesterol. Desde ahí liberan citoquinas,
    proteasas, especies reactivas de oxigeno y factores de crecimiento que potencian la reacción inflamatoria,
    desempeñando un papel esencial en la patogénesis de la enfermedad. La población creciente de macrófagos
    eventualmente entra en apoptosis y necrosis, haciendo cada vez más grande e inestable la lesión arteriosclerótica, lo que en último momento es el detonante de las complicaciones clínicas graves que pueden terminar en la muerte del paciente.

  18. SUA VIDA É UM LIVRO: UM TRABALHO INTERDISCIPLINAR ACERCA DO PAPEL DA MULHER NA COMUNIDADE

    Directory of Open Access Journals (Sweden)

    Pedro Henrique Silva

    2017-02-01

    Full Text Available Este estudo visa abordar os aspectos políticos, culturais e históricos da sociedade, com ênfase na importância do papel da mulher. A fundamentação teórica foi construída à luz dos Parâmetros Curriculares Nacionais do Ensino Médio (2006, do Currículo do Estado de São Paulo (2011 e Lustosa (2006. Tendo em vista que o ensino médio atual deve se preocupar com uma educação permanente, o que significa preparar o aluno para a vida e qualificá-lo para a cidadania, seja num futuro ambiente escolar ou profissional, espera-se que o aluno, ao realizar as atividades propostas neste projeto, adquira um significado ao seu aprendizado e reconheça a importância da figura feminina na sua sociedade. Para tanto, foi elaborado um projeto interdisciplinar entre as áreas de Linguagens, Códigos e suas Tecnologias e Ciências Humanas a fim de organizar um trabalho em que o conhecimento seja contextualizado de forma sincrônica, diacrônica e interativa.

  19. O papel das quimiocinas nas uveítes The role of chemokines in uveitis

    Directory of Open Access Journals (Sweden)

    Roberto Martins Gonçalves

    2007-03-01

    Full Text Available A inflamação é parte do processo fisiológico que visa reparar o dano tecidual causado por infecção, trauma, auto-imunidade. Quando este processo fisiológico encontra-se alterado, pode contribuir para o aumento do dano tecidual. As quimiocinas e seus receptores são importantes elementos envolvidos no processo de migração celular para os tecidos inflamados. Nas doenças oculares, principalmente nas uveítes, estas proteínas estão sendo identificadas como importantes mediadores da resposta inflamatória. Esta revisão visa discutir o papel das quimiocinas em diversas doenças oculares, dando ênfase aos processos uveíticos.Inflammation is part of the physiological process that aims at repairing the damage produced by different causes such as infection, trauma, and autoimmune disease. However, when this physiological process is not regulated, it can contribute to the increase in tissue damage. Chemokines and their receptors are major factors involved in the process of cell migration into inflamed tissues. In the ocular diseases, mainly in uveitis, such proteins have been identified as important mediators of the inflammation process. This review discusses the role of chemokines in several ocular diseases, with emphasis on the uveitic process.

  20. O papel da televisão na construção legislativa brasileira

    Directory of Open Access Journals (Sweden)

    Renata Caleffi

    2014-07-01

    Full Text Available RESUMO O processo legislativo brasileiro é colocado, algumas vezes, em questionamento, ora por ser apressado, ora por ser lento. Mais profundo que apenas o tempo em que um projeto de lei é discutido no Congresso Nacional, alguns pesquisadores conferem ainda aos veículos de comunicação uma parcela da culpa por algumas leis estarem sendo aprovadas na atualidade. O presente artigo busca encontrar alternativas para explicar a aprovação de leis no Brasil, destacando o papel da televisão como um ator social e não de uma autora legislativa. Como a televisão no Brasil é o meio mais consumido para obter informação, e o Jornal Nacional o programa de maior audiência, o telejornal é então, o objeto de pesquisa. Por meio da teoria dos múltiplos fluxos de Kingdon (2003, que dão origem às janelas de oportunidade, foi observada uma nova visão de como a construção legislativa pode ser uma estratégia política utilizada com frequência pelo Congresso Nacional e também, uma oportunidade do telejornal para pautar assuntos de caráter legislativo dentro da sociedade. Para exemplificar o processo legislativo, foram utilizadas as Leis 12.760/2012 (Lei Seca e a 12.737/2012 (Lei Carolina Dieckmann. As alternativas apresentadas neste artigo auxiliam as pesquisas interessadas em debater o papel da mídia e as relações de poder existentes entre os atores políticos nacionais, sejam em situação eletiva ou não, destacando as relações entre televisão e o Congresso e valorizando as estratégias comunicacionais dentro da política brasileira. Palavras-chave: Televisão.  Janelas da Oportunidade. Leis.  Estratégias. Política.   ABSTRACT Sometimes the Brazilian legislative process is questioned, either for being considered rushed or for being slow. Deeper than only the period in which a bill is discussed in the Congress, some researchers also blame the means of communication for some laws that have been currently adopted. This article seeks to

  1. Predictores familiares de la violencia filio-parental: el papel de la disciplina familiar

    Directory of Open Access Journals (Sweden)

    Izaskun Ibabe

    2015-05-01

    Full Text Available La violencia filio-parental es un problema social cualitativamente diferente a otros tipos de violencia familiar, porque los adolescentes dirigen la violencia hacia quienes debieran representar la autoridad y proporcionarles bienestar. El objetivo principal de este estudio era analizar el papel de las relaciones paternofiliales y la disciplina familiar en el desarrollo de conductas violentas y prosociales de los adolescentes hacia sus padres. En el estudio participaron 585 hijos/as (48% varones entre 12 y 18 años procedentes de 8 centros escolares de la Comunidad Autónoma del País Vasco. Los resultados indican que las relaciones familiares basadas en el afecto y la comunicación son las que favorecen las conductas prosociales de los hijos, y reducen las conductas violentas de éstos en el hogar. Por el contrario, las estrategias de disciplina parental coercitivas y las estrategias parcialmente coercitivas (supervisión y coste de respuesta se asocian a un mayor nivel de violencia física y psicológica de los hijos adolescentes hacia sus padres. Finalmente, se discuten las implicaciones de estos resultados en relación a la educación parental.

  2. Fronteras de Papel: Mass Media fabricando imaginarios e identidades en una ciudad-frontera mexicana

    Directory of Open Access Journals (Sweden)

    Ibán Trápaga

    2011-06-01

    Full Text Available La descripción y análisis de los elementos discursivos que conforman una región de frontera observando lo que dicen sus medios de comunicación locales es el objeto de la investigación antropológica sobre la que se sostiene este artículo. El documento aporta resultados de un estudio más amplio sobre migración y frontera en Ciudad Juárez, Chih., México y resalta las implicaciones entre comunicación de masas, cultura, ideología y poder. La metodología aplicada -Antropología de las fronteras- asume una perspectiva global y local, textual y contextual y participa de un enfoque mixto: etnográfico y lingüístico. La alteridad fronteriza aparece sometida discursivamente bajo una urdimbre de exclusión y estigmatización; es así como entiendo se construyen las fronteras internas a la nación, mediante la facturación de discursos y textos que modelan representaciones cognitivas de la realidad. Los medios de comunicación asumen un papel relevante en esta maquinación dominical.

  3. El papel de la mujer en la compra: una tipología del consumidor basada en las decisiones vacacionales

    Directory of Open Access Journals (Sweden)

    María José Barlés Arizón

    2012-01-01

    Full Text Available En el presente trabajo analizamos el papel de la mujer en el proceso de decisión de compra de las vacaciones familiares, en concreto, tratamos de identificar tipologías de mujeres en función de variables tales como las decisiones pre y post vacacionales, los estilos de vida y las variables sociodemográficas. Los datos obtenidos mediante la realización de 300 encuestas a mujeres casadas o que viven en pareja muestran tres grupos de mujeres en función del papel que toman en las decisiones vacacionales: las que participan más en las decisiones pre-vacacionales, las que tienen menos relevancia en la toma de decisiones y las que participan especialmente en las decisiones durante el viaje. También se muestra la necesidad de profundizar en el uso de los estilos de vida como variable explicativa.

  4. (Re pensando o papel do educador especial no contexto da inclusão de alunos surdos

    Directory of Open Access Journals (Sweden)

    Cinara Franco Rechico

    2012-04-01

    Full Text Available A presente pesquisa refere-se a uma das questões mais discutidas, atualmente, na área da Educação Especial: a Escola Inclusiva. Percebe-se que na caminhada de uma educação para todos e sem exclusão, é fundamental que o educador especial tenha seu papel definido para que assim esteja consciente da sua prática pedagógica. Busca-se, por meio desta, (repensar o papel do educador especial na classe do sistema regular de ensino em que haja inclusão de alunos surdos. Esta pesquisa envolve como sujeitos a educadora especial, a professora da classe regular de ensino e o instrutor surdo, os quais vivenciaram a experiência de inclusão na Escola de Ensino Fundamental Duque de Caxias, de Santa Maria – RS.

  5. Papel da dieta como fator de risco e progressäo da nefropatia diabética

    OpenAIRE

    Gross, Jorge Luiz; Azevedo, Mirela Jobim de; Zelmanovitz, Themis; Mello, Vanessa Derenji Ferreira de

    2005-01-01

    A nefropatia diabética (ND) acomete até 40% dos pacientes com diabetes melito (DM) tipo 1 e tipo 2, sendo a principal causa de insuficiênca renal crônica naqueles pacientes que ingressam em programa de tratamento de substituição renal. A dieta parece ter um papel importante no desenvolvimento da doença. Existem evidências de que não apenas a quantidade mas o tipo de proteína ingerida também está associado à ND. Poucos estudos analisaram o papel dos lipídeos da dieta na ND. Dietas hipoprotéica...

  6. Cloning of xylanase gene of Streptomyces flavogriseus in Escherichia coli and bacteriophage lambda-induced lysis for the release of cloned enzyme.

    Science.gov (United States)

    Srivastava, R; Ali, S S; Srivastava, B S

    1991-03-01

    The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII fragment and coded for 18,000 Mr xylanase.

  7. Diffusely Adhering Escherichia coli Strains Induce Attaching and Effacing Phenotypes and Secrete Homologs of Esp Proteins

    OpenAIRE

    Beinke, Christina; Laarmann, Sven; Wachter, Clemens; Karch, Helge; Greune, Lilo; Schmidt, M. Alexander

    1998-01-01

    Recent epidemiological studies indicate that Escherichia coli strains which exhibit the diffuse-adherence phenotype (DAEC strains) represent a potential cause of diarrhea in infants. We investigated the interaction of DAEC strains isolated from diarrhea patients in Brazil and in Germany with epithelial cells in tissue culture. The investigated strains were identified as DAEC strains by (i) their attachment pattern, (ii) presence of genes associated with the Dr family of adhesins, and (iii) la...

  8. Susceptibilidad a desinfectantes de Trichomonas vaginalis y Escherichia coli presentes en fómites experimentales

    Directory of Open Access Journals (Sweden)

    Fernando Anaya Velázquez

    2014-01-01

    Full Text Available Las enfermedades infecciosas provocadas por microorganismos patógenos se pueden transmitir directa o indirectamente. El protozoario Trichomonas vaginalis y la bacteria Escherichia coli son patógenos para el ser humano que pueden ser trans - mitidos por fómites. Existe una gama de productos para desinfectar, pero no existe información sobre el efecto completo de su eficacia en forma comparativa sobre bacterias o tricomonas en interacción con diferentes tipos de materiales. En este trabajo, se analizó el efecto de algunos desinfectantes sobre la viabilidad de Escherichia coli cepa 055 y Trichomonas vaginalis cepa GT-21, depositadas sobre diferentes tipos de materiales. Se diseñó un sistema in vitro con fómites experi - mentales para estudiar el efecto sobre estos patógenos. Se encontró que las tricomonas permanecen viables hasta ocho horas, y las bacterias hasta 24 horas en madera, papel, tela y plástico. Sin embargo, ambos microorganismos sobreviven solamente entre 1 y 4 horas sobre vidrio o metal. Tanto las bacterias como las tricomonas son inhibidas por los desinfec - tantes probados, siendo los más efectivos el etanol a 70% y el producto comercial Lysol ® .

  9. Autogenous regulation of Escherichia coli polynucleotide phosphorylase expression revisited.

    Science.gov (United States)

    Carzaniga, Thomas; Briani, Federica; Zangrossi, Sandro; Merlino, Giuseppe; Marchi, Paolo; Dehò, Gianni

    2009-03-01

    The Escherichia coli polynucleotide phosphorylase (PNPase; encoded by pnp), a phosphorolytic exoribonuclease, posttranscriptionally regulates its own expression at the level of mRNA stability and translation. Its primary transcript is very efficiently processed by RNase III, an endonuclease that makes a staggered double-strand cleavage about in the middle of a long stem-loop in the 5'-untranslated region. The processed pnp mRNA is then rapidly degraded in a PNPase-dependent manner. Two non-mutually exclusive models have been proposed to explain PNPase autogenous regulation. The earlier one suggested that PNPase impedes translation of the RNase III-processed pnp mRNA, thus exposing the transcript to degradative pathways. More recently, this has been replaced by the current model, which maintains that PNPase would simply degrade the promoter proximal small RNA generated by the RNase III endonucleolytic cleavage, thus destroying the double-stranded structure at the 5' end that otherwise stabilizes the pnp mRNA. In our opinion, however, the first model was not completely ruled out. Moreover, the RNA decay pathway acting upon the pnp mRNA after disruption of the 5' double-stranded structure remained to be determined. Here we provide additional support to the current model and show that the RNase III-processed pnp mRNA devoid of the double-stranded structure at its 5' end is not translatable and is degraded by RNase E in a PNPase-independent manner. Thus, the role of PNPase in autoregulation is simply to remove, in concert with RNase III, the 5' fragment of the cleaved structure that both allows translation and prevents the RNase E-mediated PNPase-independent degradation of the pnp transcript.

  10. Autogenous Regulation of Escherichia coli Polynucleotide Phosphorylase Expression Revisited▿ †

    Science.gov (United States)

    Carzaniga, Thomas; Briani, Federica; Zangrossi, Sandro; Merlino, Giuseppe; Marchi, Paolo; Dehò, Gianni

    2009-01-01

    The Escherichia coli polynucleotide phosphorylase (PNPase; encoded by pnp), a phosphorolytic exoribonuclease, posttranscriptionally regulates its own expression at the level of mRNA stability and translation. Its primary transcript is very efficiently processed by RNase III, an endonuclease that makes a staggered double-strand cleavage about in the middle of a long stem-loop in the 5′-untranslated region. The processed pnp mRNA is then rapidly degraded in a PNPase-dependent manner. Two non-mutually exclusive models have been proposed to explain PNPase autogenous regulation. The earlier one suggested that PNPase impedes translation of the RNase III-processed pnp mRNA, thus exposing the transcript to degradative pathways. More recently, this has been replaced by the current model, which maintains that PNPase would simply degrade the promoter proximal small RNA generated by the RNase III endonucleolytic cleavage, thus destroying the double-stranded structure at the 5′ end that otherwise stabilizes the pnp mRNA. In our opinion, however, the first model was not completely ruled out. Moreover, the RNA decay pathway acting upon the pnp mRNA after disruption of the 5′ double-stranded structure remained to be determined. Here we provide additional support to the current model and show that the RNase III-processed pnp mRNA devoid of the double-stranded structure at its 5′ end is not translatable and is degraded by RNase E in a PNPase-independent manner. Thus, the role of PNPase in autoregulation is simply to remove, in concert with RNase III, the 5′ fragment of the cleaved structure that both allows translation and prevents the RNase E-mediated PNPase-independent degradation of the pnp transcript. PMID:19136586

  11. Occurrence of intestinal and extraintestinal virulence genes in Escherichia coli isolates from rainwater tanks in Southeast Queensland, Australia.

    Science.gov (United States)

    Ahmed, W; Hodgers, L; Masters, N; Sidhu, J P S; Katouli, M; Toze, S

    2011-10-01

    In this study, 200 Escherichia coli isolates from 22 rainwater tank samples in Southeast Queensland, Australia, were tested for the presence of 20 virulence genes (VGs) associated with intestinal and extraintestinal pathotypes. In addition, E. coli isolates were also classified into phylogenetic groups based on the detection of the chuA, yjaA, and TSPE4.C2 genes. Of the 22 rainwater tanks, 8 (36%) and 5 (23%) were positive for the eaeA (belonging to enteropathogenic E. coli [EPEC] and Shiga-toxigenic E. coli [STEC]) and ST1 (belonging to enterotoxigenic E. coli [ETEC]) genes, respectively. VGs (cdtB, cvaC, ibeA, kpsMT allele III, PAI, papAH, and traT) belonging to extraintestinal pathogenic E. coli (ExPEC) were detected in 15 (68%) of the 22 rainwater tanks. Of the 22 samples, 17 (77%) and 11 (50%) contained E. coli belonging to phylogenetic groups A and B1, respectively. Similarly, 10 (45%) and 16 (72%) contained E. coli belonging to phylogenetic groups B2 and D, respectively. Of the 96 of the 200 strains from 22 tanks that were VG positive, 40 (42%) were carrying a single VG, 36 (37.5%) were carrying two VGs, 17 (18%) were carrying three VGs, and 3 (3%) had four or more VGs. This study reports the presence of multiple VGs in E. coli strains belonging to the STEC, EPEC, ETEC, and ExPEC pathotypes in rainwater tanks. The public health risks associated with potentially clinically significant E. coli in rainwater tanks should be assessed, as the water is used for drinking and other, nonpotable purposes. It is recommended that rainwater be disinfected using effective treatment procedures such as filtration, UV disinfection, or simply boiling prior to drinking.

  12. Papel de raquis de banano en Costa Rica: historia y aspectos técnicos, periodo de 1976 a 2002,

    OpenAIRE

    2012-01-01

    Se adjunta el Primer Capítulo del Libro Papel de raquis de banano en Costa Rica.  Historia y aspectos técnicos, periodo de 1976 a 2002. Revisión bibliográfica. Residuos de la actividad bananera y uso de las fibras del raquis de banano en la producción de pulpa celulósica.

  13. El papel de la función jurisdiccional en los casos de la moral pública

    Directory of Open Access Journals (Sweden)

    Carlos Manuel Rosales

    2013-11-01

    Full Text Available El presente trabajo analizará el fundamento moral para la aplicación de sanciones por las conductas que alteran y lesionan el orden público. Por lo que se estudiará el papel de los jueces al valorar y juzgar esos actos, bajo el principio del daño a la moral pública.

  14. Chromosomal features of Escherichia coli serotype O2:K2, an avian pathogenic E. coli

    DEFF Research Database (Denmark)

    Jørgensen, Steffen L; Kudirkiene, Egle; Li, Lili

    2017-01-01

    Escherichia coli causing infection outside the gastrointestinal system are referred to as extra-intestinal pathogenic E. coli. Avian pathogenic E. coli is a subgroup of extra-intestinal pathogenic E. coli and infections due to avian pathogenic E. coli have major impact on poultry production economy...... and welfare worldwide. An almost defining characteristic of avian pathogenic E. coli is the carriage of plasmids, which may encode virulence factors and antibiotic resistance determinates. For the same reason, plasmids of avian pathogenic E. coli have been intensively studied. However, genes encoded...... by the chromosome may also be important for disease manifestation and antimicrobial resistance. For the E. coli strain APEC_O2 the plasmids have been sequenced and analyzed in several studies, and E. coli APEC_O2 may therefore serve as a reference strain in future studies. Here we describe the chromosomal features...

  15. Free RNA polymerase in Escherichia coli.

    Science.gov (United States)

    Patrick, Michael; Dennis, Patrick P; Ehrenberg, Mans; Bremer, Hans

    2015-12-01

    The frequencies of transcription initiation of regulated and constitutive genes depend on the concentration of free RNA polymerase holoenzyme [Rf] near their promoters. Although RNA polymerase is largely confined to the nucleoid, it is difficult to determine absolute concentrations of [Rf] at particular locations within the nucleoid structure. However, relative concentrations of free RNA polymerase at different growth rates, [Rf]rel, can be estimated from the activities of constitutive promoters. Previous studies indicated that the rrnB P2 promoter is constitutive and that [Rf]rel in the vicinity of rrnB P2 increases with increasing growth rate. Recently it has become possible to directly visualize Rf in growing Escherichia coli cells. Here we examine some of the important issues relating to gene expression based on these new observations. We conclude that: (i) At a growth rate of 2 doublings/h, there are about 1000 free and 2350 non-specifically DNA-bound RNA polymerase molecules per average cell (12 and 28%, respectively, of 8400 total) which are in rapid equilibrium. (ii) The reversibility of the non-specific binding generates more than 1000 free RNA polymerase molecules every second in the immediate vicinity of the DNA. Of these, most rebind non-specifically to the DNA within a few ms; the frequency of non-specific binding is at least two orders of magnitude greater than specific binding and transcript initiation. (iii) At a given amount of RNA polymerase per cell, [Rf] and the density of non-specifically DNA-bound RNA polymerase molecules along the DNA both vary reciprocally with the amount of DNA in the cell. (iv) At 2 doublings/h an E. coli cell contains, on the average, about 1 non-specifically bound RNA polymerase per 9 kbp of DNA and 1 free RNA polymerase per 20 kbp of DNA. However some DNA regions (i.e. near active rRNA operons) may have significantly higher than average [Rf].

  16. The anaerobic (Class III) ribonucleotide reductase from Lactococcus lactis : Catalytic properties and allosteric regulation of the pure enzyme system

    NARCIS (Netherlands)

    Torrents, Eduard; Buist, Girbe; Liu, Aimin; Eliasson, Rolf; Kok, Jan; Gibert, Isidre; Gräslund, Astrid; Reichard, Peter

    2000-01-01

    Lactococcus lactis contains an operon with the genes (nrdD and nrdG) for a class III ribonucleotide reductase, Strict anaerobic growth depends on the activity of these genes. Both were sequenced, cloned, and overproduced in Escherichia coli, The corresponding proteins, NrdD and NrdG, were purified c

  17. El papel de algunas instituciones sociales en el derecho a decidir sobre la regulación de la fecundidad

    Directory of Open Access Journals (Sweden)

    Ivonne Szasz

    2002-01-01

    Full Text Available El artículo presenta algunas posiciones que se debaten en la bibliografía actual sobre el papel de las instituciones sociales en la toma de decisiones sobre los eventos reproductivos y su regulación. Se plantean algunos términos del debate sobre los derechos reproductivos y el papel de las instituciones públicas de salud, distinguiendo entre el desempeño de esas instituciones en distintos tipos de escenarios estatales. Se analiza también el papel que pueden desempeñar las organizaciones no gubernamentales en la promoción del derecho a decidir, distinguiendo entre las ONG vinculadas con movimientos sociales de base y las organizaciones asistenciales asociadas con grupos de poder. Un pequeño apartado se refiere a la importancia de la familia y las relaciones de parentesco en la toma de decisiones reproductivas, y por último, se señalan las potencialidades del ámbito escolar tanto en la reproducción como en la transformación de relaciones de género y generacionales de tipo autoritario que dificultan el desarrollo de la autonomía personal y de la reflexión crítica.

  18. Texto ilustrado o imágenes textualizadas. Un acercamiento al Papel Periódico Ilustrado desde la relación entre arte y literatura

    National Research Council Canada - National Science Library

    Diana Carolina Toro Henao

    2015-01-01

      This text presents a re-reading of researches about the Papel Periódico Ilustrado and about the way the relationship between texts and images have been addressed, which it presents as an illustrated literary periodical publication...

  19. Estudo de Mercado em Duas Áreas Específicas de Aplicação de Papel Sintético

    Directory of Open Access Journals (Sweden)

    Almeida Marcelo C.

    2002-01-01

    Full Text Available O objetivo deste trabalho é identificar a demanda potencial para papel sintético no Brasil. Neste primeiro levantamento, foram consideradas aplicações específicas como painéis de propagandas veiculadas através de quadros de 'outdoor', ônibus de transporte coletivo urbano, táxis e confecção de documentos pessoais. Uma demanda anual entre 2.500 toneladas e 2.800 toneladas seria observada se todas as aplicações abordadas neste trabalho utilizassem o papel sintético em substituição ao papel celulósico. O mercado para o papel sintético, ainda pouco difundido no Brasil, pode ter uma demanda anual contínua e de volume considerável, se houver investimento em marketing para familiarizar os consumidores com o material.

  20. La Cultura Organizacional y las Estrategias Competitivas en la industria de celulosa, cartón y papel en México

    National Research Council Canada - National Science Library

    Francisco Martín Villarreal Solís; José Gerardo Ignacio Gómez Romero; María Deyanira Villarreal Solís

    2014-01-01

    El objetivo de este trabajo es analizar la posible relación entre el grado de flexibilidad o rigidez de la Cultura Organizacional y el uso de las Estrategias Competitivas en la industria del papel...

  1. Thiophene metabolism by E. coli

    Energy Technology Data Exchange (ETDEWEB)

    Clark, D.P.

    1991-01-01

    The objective of this project is to investigate the mechanism of degradation of sulfur-containing heterocyclic molecules by mutant strains of Escherichia coli K-12. We have previously isolated multiple mutants of E. coli which had gained the capacity to oxidize thiophene compounds and their furan analogs. We have focused on the thdA mutation in our subsequent research, as this appears to be in a regulatory gene central to the thiophene/furan oxidation system. The thdF gene appears to be more directly involved in the oxidation reactions, whereas thdC and thdD are apparently required for increased protection against the toxic effects of thiophene and furan compounds. 4 tabs.

  2. Multiplex PCR Assay for Identification of Human Diarrheagenic Escherichia coli

    OpenAIRE

    Toma, Claudia; Lu, Yan; Higa, Naomi; Nakasone, Noboru; Isabel CHINEN; Baschkier, Ariela; Rivas, Marta; Iwanaga, Masaaki

    2003-01-01

    A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.

  3. Multiplex PCR Assay for Identification of Human Diarrheagenic Escherichia coli

    OpenAIRE

    2003-01-01

    A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.

  4. Enterohemorrhagic E. coli (EHEC) pathogenesis

    OpenAIRE

    Vanessa eSperandio; Y eNguyen

    2012-01-01

    Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 is a human pathogen responsible for outbreaks of bloody diarrhea and hemolytic uremic syndrome (HUS) worldwide. Conventional antimicrobials trigger an SOS response in EHEC that promotes the release of the potent Shiga toxin that is responsible for much of the morbidity and mortality associated with EHEC infection. Cattle are a natural reservoir of EHEC, and approximately 75% of EHEC outbreaks are linked to the consumption of contamina...

  5. Cr(III), Fe(III) and Co(III) complexes of tetradentate (ONNO) Schiff base ligands: Synthesis, characterization, properties and biological activity

    Science.gov (United States)

    Keskioğlu, Eren; Gündüzalp, Ayla Balaban; Çete, Servet; Hamurcu, Fatma; Erk, Birgül

    2008-08-01

    A series of metal complexes were synthesized from equimolar amounts of Schiff bases: 1,4-bis[3-(2-hydroxy-1-naphthaldimine)propyl]piperazine (bappnaf) and 1,8-bis[3-(2-hydroxy-1-naphthaldimine)- p-menthane (damnaf) with metal chlorides. All of synthesized compounds were characterized by elemental analyses, spectral (UV-vis, IR, 1H- 13C NMR, LC-MS) and thermal (TGA-DTA) methods, magnetic and conductance measurements. Schiff base complexes supposed in tetragonal geometry have the general formula [M(bappnaf or damnaf)]Cl· nH 2O, where M = Cr(III), Co(III) and n = 2, 3. But also Fe(III) complexes have octahedral geometry by the coordination of two water molecules and the formula is [Fe(bappnaf or damnaf)(H 2O) 2]Cl. The changes in the selected vibration bands in FT-IR indicate that Schiff bases behave as (ONNO) tetradentate ligands and coordinate to metal ions from two phenolic oxygen atoms and two azomethine nitrogen atoms. Conductance measurements suggest 1:1 electrolytic nature of the metal complexes. The synthesized compounds except bappnaf ligand have the antimicrobial activity against the bacteria: Escherichia coli (ATCC 11230), Yersinia enterocolitica (ATCC 1501), Bacillus magaterium (RSKK 5117), Bacillus subtilis (RSKK 244), Bacillus cereus (RSKK 863) and the fungi: Candida albicans (ATCC 10239). These results have been considerably interest in piperazine derivatives due to their significant applications in antimicrobial studies.

  6. Cr(III), Fe(III) and Co(III) complexes of tetradentate (ONNO) Schiff base ligands: synthesis, characterization, properties and biological activity.

    Science.gov (United States)

    Keskioğlu, Eren; Gündüzalp, Ayla Balaban; Cete, Servet; Hamurcu, Fatma; Erk, Birgül

    2008-08-01

    A series of metal complexes were synthesized from equimolar amounts of Schiff bases: 1,4-bis[3-(2-hydroxy-1-naphthaldimine)propyl]piperazine (bappnaf) and 1,8-bis[3-(2-hydroxy-1-naphthaldimine)-p-menthane (damnaf) with metal chlorides. All of synthesized compounds were characterized by elemental analyses, spectral (UV-vis, IR, (1)H-(13)C NMR, LC-MS) and thermal (TGA-DTA) methods, magnetic and conductance measurements. Schiff base complexes supposed in tetragonal geometry have the general formula [M(bappnaf or damnaf)]Cl.nH(2)O, where M=Cr(III), Co(III) and n=2, 3. But also Fe(III) complexes have octahedral geometry by the coordination of two water molecules and the formula is [Fe(bappnaf or damnaf)(H(2)O)(2)]Cl. The changes in the selected vibration bands in FT-IR indicate that Schiff bases behave as (ONNO) tetradentate ligands and coordinate to metal ions from two phenolic oxygen atoms and two azomethine nitrogen atoms. Conductance measurements suggest 1:1 electrolytic nature of the metal complexes. The synthesized compounds except bappnaf ligand have the antimicrobial activity against the bacteria: Escherichia coli (ATCC 11230), Yersinia enterocolitica (ATCC 1501), Bacillus magaterium (RSKK 5117), Bacillus subtilis (RSKK 244), Bacillus cereus (RSKK 863) and the fungi: Candida albicans (ATCC 10239). These results have been considerably interest in piperazine derivatives due to their significant applications in antimicrobial studies.

  7. Robust growth of Escherichia coli.

    Science.gov (United States)

    Wang, Ping; Robert, Lydia; Pelletier, James; Dang, Wei Lien; Taddei, Francois; Wright, Andrew; Jun, Suckjoon

    2010-06-22

    The quantitative study of the cell growth has led to many fundamental insights in our understanding of a wide range of subjects, from the cell cycle to senescence. Of particular importance is the growth rate, whose constancy represents a physiological steady state of an organism. Recent studies, however, suggest that the rate of elongation during exponential growth of bacterial cells decreases cumulatively with replicative age for both asymmetrically and symmetrically dividing organisms, implying that a "steady-state" population consists of individual cells that are never in a steady state of growth. To resolve this seeming paradoxical observation, we studied the long-term growth and division patterns of Escherichia coli cells by employing a microfluidic device designed to follow steady-state growth and division of a large number of cells at a defined reproductive age. Our analysis of approximately 10(5) individual cells reveals a remarkable stability of growth whereby the mother cell inherits the same pole for hundreds of generations. We further show that death of E. coli is not purely stochastic but is the result of accumulating damages. We conclude that E. coli, unlike all other aging model systems studied to date, has a robust mechanism of growth that is decoupled from cell death.

  8. Requerimientos establecidos por las normas ISO para papeles permanentes Established requirements for ISO standards for permanent papers

    Directory of Open Access Journals (Sweden)

    Juan Facundo Araujo

    2011-06-01

    Full Text Available El patrimonio documental nacional e internacional se encuentra en su gran mayoría en soporte papel. A partir de la industrialización en la manufactura de dicho material se ha producido papel con niveles de acidez, que sumado a otros factores, ha sido causante del deterioro de este tipo de patrimonio documental. En las últimas décadas, con la incorporación de nuevos procesos dentro de la producción papelera se ha resuelto en parte dicha problemática. El organismo internacional de normalización ISO ha redactado dos normas al respecto: la norma ISO 9706 e ISO 11108. Con la difusión y recomendación de uso del papel permanente para la conservación del patrimonio documental a largo plazo, se hace imprescindible conocer dicha normativa e informarse al respecto. En el presente artículo se describen las variables en la fabricación del papel que influyen en la permanencia de los papeles. También se determinan los conceptos y antecedentes que rodean ambas normas ISO, así como las características principales y partes que componen las mismas. Como anexo se consigna un glosario con los principales términos técnicos utilizados.Most of the national and international documentary heritage are on paper. From industrialization in the manufacture of such material, paper with acidity levels has been produced which, together with other factors, has been causing the deterioration of this type of documentary heritage. In recent decades, with the incorporation of new paper production processes this problem has been partly solved. The international standards organism ISO has drafted two rules in this connection: ISO 9706 and ISO 11108. With the spread and recommendation for the use of permanent paper for preservation of documentary heritage in the long term, it is essential to know the standards and be well informed. This article describes the variables in the manufacture of paper that influence the permanence of the papers. It also identifies the

  9. Formação profissional no SUS: o papel da Atenção Básica em Saúde na perspectiva docente

    Directory of Open Access Journals (Sweden)

    Ricardo Corrêa Ferreira

    Full Text Available A educação médica tem sofrido profundas críticas quanto à necessidade de diversificar os cenários de ensino-aprendizagem para que se construam novos currículos e sujeitos, possibilitando-lhes a inser ção num processo pedagógico reflexivo e dinâmico. O objetivo desta pesquisa é analisar a percepção que docentes da Unidade de Prática Profissional da Faculdade de Medicina de Marília têm acerca do papel que a Atenção Básica de Saúde (ABS desempenha na formação profissional dos estudantes. Realizou-se um estudo qualitativo por meio de grupos focais com análise de discurso dos sujeitos entrevistados. Construíram-se três categorias: o papel da ABS na construção do SUS; o papel do SUS na formação profissional de novos sujeitos; o papel docente na construção profissional de novos sujeitos. Considera-se fundamental o papel exercido pela ABS na formação dos profissionais de saúde, notadamente profissionais críticos e reflexivos, destacando-se o papel transformador e emancipador que o docente exerce nessa formação.

  10. Sterilization by Cooling in Isochoric Conditions: The Case of Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Samuel Salinas-Almaguer

    Full Text Available High hydrostatic pressure (HHP affects the structure, metabolism and survival of micro-organisms including bacteria. For this reason HHP is a promising treatment in the food industry. The aim of this work is to evaluate the effect of high pressure, under isochoric cooling conditions, on Escherichia coli, where such high pressure develops due to the fact water cannot expand. We combine survival curves obtained by spectrophotometry and images of atomic force microscopy in this study. Our results show that cooling at -20 and -30°C leads to a partial destruction of a Escherichia coli population. However, cooling at -15°C causes a total extermination of bacteria. This intriguing result is explained by the phase diagram of water. In the first case, the simultaneous formation of ice III and ice Ih crystals provides a safe environment for bacteria. In the second case (-15°C Escherichia coli remains in a metastable and amorphous free-of-crystals liquid subjected to high pressure. Our work is the first experimental study carried out to inactivate Escherichia coli under isochoric cooling conditions. Unlike HHP, which is based on the application of an external load to augment the pressure, this technique only requires cooling. The method could be used for annihilation of other Escherichia coli strains and perhaps other micro-organisms.

  11. El papel del Sr en la manufactura de compositos colados. // The Sr influence in manufactured composites.

    Directory of Open Access Journals (Sweden)

    J. A. García-Hinojosa

    2002-09-01

    Full Text Available Es bien sabido que los materiales de alta tecnología obtenidos por diferentes rutas de manufactura, deben ser investigadocon mayor detalle y profundidad, con el propósito de identificar y entender los fenómenos involucrados durante lafabricación de los mismos, y de esta manera predecir el comportamiento durante posteriores etapas de manufactura o de sudesempeño en componentes finales. Los materiales compuestos de matriz metálica ligera base aluminio, obtenidos porrutas económicas de fabricación, como las de fase líquida o de fundición, representan una prometedora opción para hacerlascompetitivas contra los materiales tradicionales, en el campo de la diversidad de aplicaciones ingenieriles.Considerando lo anterior, en este trabajo se presentan los resultados de la manufactura y caracterización microestructural demateriales compuestos colados con matriz base aluminio A356 reforzados con 10 % en vol. de partículas de SiC condiferentes adiciones de Sr. El propósito fue determinar acerca del papel que desempeña la adición de Sr. Para ello sefabricaron compositos con diferentes contenidos de Sr, los cuales fueron caracterizados microestructuralmente en aspectosrelacionados con la distribución de partículas en la matriz, la presencia de conglomerados, el espaciamiento interdendríticosecundario, morfología del Si eutéctico, así como estudios interfaciales en la región matriz cerámico. Los resultadosobtenido se interpretaron en el sentido de explicar el papel que desempeña la cantidad de Sr sobre estos materialescompuestos.Palabras claves: Composite, fase liquida, aplicaciones ingenieriles.___________________________________________________________________AbstractIt is well known that the materials of high technology obtained by different factory routes, they should be investigated withbigger detail with the purpose to identify and understand the phenomenons involved during the production and predict thebehavior during later

  12. PREFACE: Quantum Optics III

    Science.gov (United States)

    Orszag, M.; Retamal, J. C.; Saavedra, C.; Wallentowitz, S.

    2007-06-01

    All the 50 years of conscious pondering did not bring me nearer to an answer to the question `what is light quanta?'. Nowadays, every rascal believes, he knows it, however, he is mistaken. (A Einstein, 1951 in a letter to M Besso) Quantum optics has played a key role in physics in the last several decades. On the other hand, in these early decades of the information age, the flow of information is becoming more and more central to our daily life. Thus, the related fields of quantum information theory as well as Bose-Einstein condensation have acquired tremendous importance in the last couple of decades. In Quantum Optics III, a fusion of these fields appears in a natural way. Quantum Optics III was held in Pucón, Chile, in 27-30 of November, 2006. This beautiful location in the south of Chile is near the lake Villarrica and below the snow covered volcano of the same name. This fantastic environment contributed to a relaxed atmosphere, suitable for informal discussion and for the students to have a chance to meet the key figures in the field. The previous Quantum Optics conferences took place in Santiago, Chile (Quantum Optics I, 2000) and Cozumel, Mexico (Quantum Optics II, 2004). About 115 participants from 19 countries attended and participated in the meeting to discuss a wide variety of topics such as quantum-information processing, experiments related to non-linear optics and squeezing, various aspects of entanglement including its sudden death, correlated twin-photon experiments, light storage, decoherence-free subspaces, Bose-Einstein condensation, discrete Wigner functions and many more. There was a strong Latin-American participation from Argentina, Brazil, Chile, Colombia, Peru, Uruguay, Venezuela and Mexico, as well as from Europe, USA, China, and Australia. New experimental and theoretical results were presented at the conference. In Latin-America a quiet revolution has taken place in the last twenty years. Several groups working in quantum optics and

  13. Papel dos pais no desenvolvimento de jovens futebolistas Parents role in the development of soccer players

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Moraes

    2004-01-01

    Full Text Available Este estudo investigou o papel dos pais no desenvolvimento de atletas jovens de futebol. Foram voluntários nesse projeto 20 pais e 12 filhos jogadores, entre as idades de 15 e 18 anos, participantes da temporada 2000 do Campeonato Mineiro. Utilizou-se uma abordagem quantitativa e qualitativa, através de formulários, questionários e entrevistas semi-estruturadas de aprofundamento. Constatou-se que os pais tinham pouco envolvimento nos treinamentos e competições dos atletas, não alteraram a rotina familiar em função dos treinamentos dos mesmos. O relativo apoio dos pais não prejudicou o progresso dos filhos devido os pais permitirem os mesmos praticarem o futebol livremente. Outro aspecto importante foi o progresso dos filhos devido à paixão, à intensidade e freqüência de prática, além do apelo financeiro que o futebol profissional evoca no Brasil. Esses resultados indicam a necessidade de precauções quando se considerar paradigmas de primeiro mundo em outras culturas na qual exista restrição contextual.This study investigated the role of parents in the development of soccer players. Twenty parents and 12 soccer players, between 15 and 18 years old participated in the study. Both quantitative and qualitative approaches were used by administrating questionnaires forms and interviews. It was observed that few changes occurred in the family routines and that parents were minimally involved in their sons' sport activities. This did not appear to be a constraint for their sons' development because of their passion for soccer, the total amount of practice, and a potential lucrative professional career. Researchers should carefully adopt important paradigms from first world countries to another country with contextual differences.

  14. Propuesta sobre el papel del profesional de enfermería en salud ocupacional

    Directory of Open Access Journals (Sweden)

    Useche Mora Luz Graciela

    1996-06-01

    Full Text Available

    El presente artículo sobre el papel del profesional de enfermería en salud ocupacional, constituye un intento de formulación de una propuesta, que aporte a la construcción de un modelo de intervención de enfermería en salud ocupacional en Colombia. La importancia de este estudio se basa en la necesidad de proporcionar a profesionales y estudiantes un marco de referencia que oriente la práctica de enfermería en salud ocupacional, ya que los estudios y escritos sobre el tema son extremadamente escasos en nuestro país. En este artículo se explica el significado, los objetivos, el sujeto de atención y la metodología de trabajo del profesional en este campo, en forma sistemática se organizan los componentes de la práctica, explicitando los factores del ambiente externo e interno que intervienen en la salud del trabajador. Así mismo se describen las áreas de acción de enfermería, en las cuales el profesional interviene de diferentes maneras, para contribuir a la promoción y mantenimiento de la salud del trabajador. Se espera que el presente artículo, motive a los lectores a una reflexión crítica que redunde en la búsqueda de caminos apropiados en el logro de los objetivos de atención de enfermería en salud ocupacional, como es el bienestar del trabajador, requisito esencial para el desarrollo del país.

     

  15. O papel do fisiologista desportivo no futebol: para quê & por quê?

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Santos Silva

    2000-08-01

    Full Text Available A fisiologia desportiva ainda é considerada uma especialidade relativamente nova no futebol. A figura do fisiologista desportivo e, conseqüentemente, sua função e formação, é desconhecida pela grande maioria daqueles que estão envolvidos nesta modalidade, não sabendo caracterizar o papel desse profissional numa equipe de futebol. É importante ressaltar que o especialista desta área trabalha diretamente junto ao fisicultor, cabendo a ele funções como: 1 trabalho em equipe passando informações constantes à comissão técnica sobre as condições funcionais dos jogadores; 2 avaliação sistemática dos atletas; 3 acompanhamento longitudinal das adaptações funcionais em decorrência do treinamento dos atletas e 4 capacidade de investigação e reflexão sobre diversos aspectos do futebol. Sendo assim, o fisiologista desportivo requer amplo conhecimento de metodologias científicas de avaliação funcional e treinamento desportivo, bem como o domínio específico de conceitos bioenergéticos direcionados para o futebol. Isso permite identificar o tipo de esforço e selecionar métodos adequados para o desenvolvimento do programa de treinamento do futebolista. Concluindo, o fisiologista desportivo, em sua essência, é sobretudo um profissional de saúde que tem cada vez mais uma função educativa. Ele contribui para melhorar a informação científica que toda a comunidade esportiva deve ter sobre diversos aspectos de saúde do corpo humano e, em particular, quando submetido à realização de exercícios.

  16. LATENCIA DEL HERPESVIRUS BOVINO-1: EL PAPEL DE LOS TRANSCRITOS RELACIONADOS CON LATENCIA (RL

    Directory of Open Access Journals (Sweden)

    JULIÁN RUIZ

    2008-01-01

    Full Text Available El herpesvirus bovino-1 es un virus de distribución mundial causante de graves pérdidas económicas debidas principalmente a la disminución de la eficiencia y en los indicadores de salud y productividad de cualquier hato ganadero infectado. Luego de la infección inicial del tracto respiratorio de los animales, el virus establece un estado de latencia viral en las neuronas sensoriales del ganglio trigémino y en los centros germinales de las tonsilas faríngeas. Periódicamente, el virus es reactivado y excretado en secreciones a través de las cuales puede infectar a otros animales susceptibles. Durante dicho estado de latencia hay disminución dramática de la expresión de genes virales, llevando solo a la expresión de dos transcritos: El RNA codificado por el gen relacionado con latencia (RL y el ORF-E viral. Múltiples estudios demuestran como el RL y el ORF-E están involucrados en la regulación del complejo ciclo de latencia y reactivación de la infección. La presente revisión de literatura se enfocará en describir y analizar los distintos estudios que han llevado a dilucidar el papel jugado por el gen RL y el ORF-E, sus transcritos y sus productos proteicos en el establecimiento, man- tenimiento y reactivación de la latencia del HVB-1.

  17. LATENCIA DEL HERPESVIRUS BOVINO-1: EL PAPEL DE LOS TRANSCRITOS RELACIONADOS CON LATENCIA (RL

    Directory of Open Access Journals (Sweden)

    VERA VÍCTOR

    2008-04-01

    Full Text Available

    El herpesvirus bovino-1 es un virus de distribución mundial causante de graves pérdidas económicas debidas principalmente a la disminución de la eficiencia y en los indicadores de salud y productividad de cualquier hato ganadero infectado. Luego de la infección inicial del tracto respiratorio de los animales, el virus establece un estado de latencia viral en las neuronas sensoriales del ganglio trigémino y en los centros germinales de las tonsilas faríngeas. Periódicamente, el virus es reactivado y excretado en secreciones a través de las cuales puede infectar a otros animales susceptibles. Durante dicho estado de latencia hay disminución dramática de la expresión de genes virales, llevando solo a la expresión de dos transcritos: El RNA codificado por el gen relacionado con latencia (RL y el ORF-E viral. Múltiples estudios demuestran como el RL y el ORF-E están involucrados en la regulación del complejo ciclo de latencia y reactivación de la infección. La presente revisión de literatura se enfocará en describir y analizar los distintos estudios que han llevado a dilucidar el papel jugado por el gen RL y el ORF-E, sus transcritos y sus productos proteicos en el establecimiento, mantenimiento y reactivación de la latencia del HVB-1.

  18. Arabidopsis thaliana: uma pequena planta um grande papel Arabidopsis thaliana: a small plant a big role

    Directory of Open Access Journals (Sweden)

    Carla Andréa Delatorre

    2008-12-01

    Full Text Available Arabidopsis thaliana é uma das espécies mais utilizadas na pesquisa científica atualmente. Apesar de não apresentar importância econômica direta, esta espécie é o foco de pesquisas na área da genética, bioquímica e fisiologia. O número de trabalhos publicados sobre a mesma aumentou significativamente após o seqüenciamento de seu genoma. Apesar do grande número de estudos existe ainda muita desinformação sobre qual o seu verdadeiro papel na pesquisa científica de espécies cultivadas e de que maneira o avanço no conhecimento adquirido com A. thaliana pode auxiliar o desenvolvimento de cultivares cada vez mais resistentes, adaptados e produtivos. Os objetivos deste trabalho são discutir as razões do uso da A. thaliana como espécie modelo e a aplicabilidade deste modelo no estudo de espécies cultivadas.Arabidopsis thaliana has been the species of choice for scientific research. Despite its lack of economic importance, it has been the focus of genetic, biochemical and physiological research worldwide. The number of published articles about arabidopsis has increased substantially after its genome was sequenced, and outgrew the number of articles related to economically important species. Despite the great number of studies involving arabidopsis, there is much disinformation about the actual role of this species in crop scientific research, as well as how the breakthroughs in arabidopsis research may help to develop more adapted and productive crops. This work aims to discuss reasons for using A. thaliana as a model species and the feasibility of this model for crop studies.

  19. O papel da televisão na construção legislativa brasileira

    Directory of Open Access Journals (Sweden)

    Renata Caleffi

    2014-07-01

    Full Text Available RESUMO O processo legislativo brasileiro é colocado, algumas vezes, em questionamento, ora por ser apressado, ora por ser lento. Mais profundo que apenas o tempo em que um projeto de lei é discutido no Congresso Nacional, alguns pesquisadores conferem ainda aos veículos de comunicação uma parcela da culpa por algumas leis estarem sendo aprovadas na atualidade. O presente artigo busca encontrar alternativas para explicar a aprovação de leis no Brasil, destacando o papel da televisão como um ator social e não de uma autora legislativa. Como a televisão no Brasil é o meio mais consumido para obter informação, e o Jornal Nacional o programa de maior audiência, o telejornal é então, o objeto de pesquisa. Por meio da teoria dos múltiplos fluxos de Kingdon (2003, que dão origem às janelas de oportunidade, foi observada uma nova visão de como a construção legislativa pode ser uma estratégia política utilizada com frequência pelo Congresso Nacional e também, uma oportunidade do telejornal para pautar assuntos de caráter legislativo dentro da sociedade. Para exemplificar o processo legislativo, foram utilizadas as Leis 12.760/2012 (Lei Seca e a 12.737/2012 (Lei Carolina Dieckmann. As alternativas apresentadas neste artigo auxiliam as pesquisas interessadas em debater o papel da mídia e as relações de poder existentes entre os atores políticos nacionais, sejam em situação eletiva ou não, destacando as relações entre televisão e o Congresso e valorizando as estratégias comunicacionais dentro da política brasileira. Palavras-chave: Televisão.  Janelas da Oportunidade. Leis.  Estratégias. Política.   ABSTRACT Sometimes the Brazilian legislative process is questioned, either for being considered rushed or for being slow. Deeper than only the period in which a bill is discussed in the Congress, some researchers also blame the means of communication for some laws that have been currently adopted. This article seeks to

  20. Chromosomal features of Escherichia coli serotype O2:K2, an avian pathogenic E. coli.

    Science.gov (United States)

    Jørgensen, Steffen L; Kudirkiene, Egle; Li, Lili; Christensen, Jens P; Olsen, John E; Nolan, Lisa; Olsen, Rikke H

    2017-01-01

    Escherichia coli causing infection outside the gastrointestinal system are referred to as extra-intestinal pathogenic E. coli. Avian pathogenic E. coli is a subgroup of extra-intestinal pathogenic E. coli and infections due to avian pathogenic E. coli have major impact on poultry production economy and welfare worldwide. An almost defining characteristic of avian pathogenic E. coli is the carriage of plasmids, which may encode virulence factors and antibiotic resistance determinates. For the same reason, plasmids of avian pathogenic E. coli have been intensively studied. However, genes encoded by the chromosome may also be important for disease manifestation and antimicrobial resistance. For the E. coli strain APEC_O2 the plasmids have been sequenced and analyzed in several studies, and E. coli APEC_O2 may therefore serve as a reference strain in future studies. Here we describe the chromosomal features of E. coli APEC_O2. E. coli APEC_O2 is a sequence type ST135, has a chromosome of 4,908,820 bp (plasmid removed), comprising 4672 protein-coding genes, 110 RNA genes, and 156 pseudogenes, with an average G + C content of 50.69%. We identified 82 insertion sequences as well as 4672 protein coding sequences, 12 predicated genomic islands, three prophage-related sequences, and two clustered regularly interspaced short palindromic repeats regions on the chromosome, suggesting the possible occurrence of horizontal gene transfer in this strain. The wildtype strain of E. coli APEC_O2 is resistant towards multiple antimicrobials, however, no (complete) antibiotic resistance genes were present on the chromosome, but a number of genes associated with extra-intestinal disease were identified. Together, the information provided here on E. coli APEC_O2 will assist in future studies of avian pathogenic E. coli strains, in particular regarding strain of E. coli APEC_O2, and aid in the general understanding of the pathogenesis of avian pathogenic E. coli.

  1. Presence of Shiga toxin-producing Escherichia coli, Enteroinvasive E. coli, Enteropathogenic E. coli, and Enterotoxigenic E. coli on tomatoes from public markets in Mexico.

    Science.gov (United States)

    Gómez-Aldapa, Carlos A; Torres-Vitela, M Del Refugio; Acevedo-Sandoval, Otilio A; Rangel-Vargas, Esmeralda; Villarruel-López, Angélica; Castro-Rosas, Andjavier

    2013-09-01

    Diarrheagenic Escherichia coli pathotypes (DEP) are important foodborne pathogens in various countries, including Mexico. However, no data exist on the presence of DEP on fresh tomatoes (Solanum lycopericum) from Mexico. The frequency of fecal coliforms (FC), E. coli, and DEP were determined for two tomato varieties. One hundred samples of a saladette tomato variety and 100 samples of a red round tomato variety were collected from public markets in Pachuca, Mexico. Each tomato sample consisted of four whole tomatoes. For the 100 saladette samples, coliform bacterial, FC, E. coli, and DEP were identified in 100, 70, 60, and 10% of samples, respectively. For the 100 red round samples, coliform bacterial, FC, E. coli, and DEP were identified in 100, 75, 65, and 11% of samples, respectively. Identified DEP included Shiga toxin-producing E. coli (STEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC), and enterotoxigenic E. coli (ETEC). STEC were isolated from 6% of saladette samples and 5% of red round samples. ETEC were isolated from 3% of saladette samples and 4% of red round samples. EPEC were isolated from 2% of saladette samples and 3% of red round samples, and EIEC were isolated from 1% of saladette samples. Both STEC and ETEC were identified in two saladette samples and 1 red round sample. E. coli O157:H7 was not detected in any STEC-positive samples.

  2. Escherichia coli in Europe: An Overview

    Directory of Open Access Journals (Sweden)

    Nerino Allocati

    2013-11-01

    Full Text Available Escherichia coli remains one of the most frequent causes of several common bacterial infections in humans and animals. E. coli is the prominent cause of enteritis, urinary tract infection, septicaemia and other clinical infections, such as neonatal meningitis. E. coli is also prominently associated with diarrhoea in pet and farm animals. The therapeutic treatment of E. coli infections is threatened by the emergence of antimicrobial resistance. The prevalence of multidrug-resistant E. coli strains is increasing worldwide principally due to the spread of mobile genetic elements, such as plasmids. The rise of multidrug-resistant strains of E. coli also occurs in Europe. Therefore, the spread of resistance in E. coli is an increasing public health concern in European countries. This paper summarizes the current status of E. coli strains clinically relevant in European countries. Furthermore, therapeutic interventions and strategies to prevent and control infections are presented and discussed. The article also provides an overview of the current knowledge concerning promising alternative therapies against E. coli diseases.

  3. Annual Surveillance Summary: Escherichia coli (E. coli) Infections in the Military Health System (MHS), 2016

    Science.gov (United States)

    2017-06-30

    Annual Surveillance Summary: Escherichia coli (E. coli) Infections in the Military Health System (MHS...and prevalence among all beneficiaries seeking care within the Military Health System (MHS). This report describes demographics, clinical...linked to assess descriptive and clinical factors related to E. coli. Health Level 7 (HL7)-formatted Composite Health Care System (CHCS) microbiology data

  4. Structure of the Cyclomodulin Cif from Pathogenic Escherichia coli

    Science.gov (United States)

    Hsu, Yun; Jubelin, Gregory; Taieb, Frédéric; Nougayrède, Jean-Philippe; Oswald, Eric; Stebbins, C. Erec

    2009-01-01

    Bacterial pathogens have evolved a sophisticated arsenal of virulence factors to modulate host cell biology. Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) use a type III protein secretion system (T3SS) to inject microbial proteins into host cells. The T3SS effector cycle inhibiting factor (Cif) produced by EPEC and EHEC is able to block host eukaryotic cell-cycle progression. We present here a crystal structure of Cif, revealing it to be a divergent member of the superfamily of enzymes including cysteine proteases and acetyltransferases that share a common catalytic triad. Mutation of these conserved active site residues abolishes the ability of Cif to block cell-cycle progression. Finally, we demonstrate that irreversible cysteine protease inhibitors do not abolish the Cif cytopathic effect, suggesting that another enzymatic activity may underlie the biological activity of this virulence factor. PMID:18845161

  5. Structure of the Cyclomodulin Cif from Pathogenic Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, Y.; Jubelin, G; Taieb, F; Nougayrède, J; Oswald, E; Stebbins, C

    2008-01-01

    Bacterial pathogens have evolved a sophisticated arsenal of virulence factors to modulate host cell biology. Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) use a type III protein secretion system (T3SS) to inject microbial proteins into host cells. The T3SS effector cycle inhibiting factor (Cif) produced by EPEC and EHEC is able to block host eukaryotic cell-cycle progression. We present here a crystal structure of Cif, revealing it to be a divergent member of the superfamily of enzymes including cysteine proteases and acetyltransferases that share a common catalytic triad. Mutation of these conserved active site residues abolishes the ability of Cif to block cell-cycle progression. Finally, we demonstrate that irreversible cysteine protease inhibitors do not abolish the Cif cytopathic effect, suggesting that another enzymatic activity may underlie the biological activity of this virulence factor.

  6. Structure of the cyclomodulin Cif from pathogenic Escherichia coli.

    Science.gov (United States)

    Hsu, Yun; Jubelin, Gregory; Taieb, Frédéric; Nougayrède, Jean-Philippe; Oswald, Eric; Stebbins, C Erec

    2008-12-12

    Bacterial pathogens have evolved a sophisticated arsenal of virulence factors to modulate host cell biology. Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) use a type III protein secretion system (T3SS) to inject microbial proteins into host cells. The T3SS effector cycle inhibiting factor (Cif) produced by EPEC and EHEC is able to block host eukaryotic cell-cycle progression. We present here a crystal structure of Cif, revealing it to be a divergent member of the superfamily of enzymes including cysteine proteases and acetyltransferases that share a common catalytic triad. Mutation of these conserved active site residues abolishes the ability of Cif to block cell-cycle progression. Finally, we demonstrate that irreversible cysteine protease inhibitors do not abolish the Cif cytopathic effect, suggesting that another enzymatic activity may underlie the biological activity of this virulence factor.

  7. Celestine III and the North

    DEFF Research Database (Denmark)

    Nielsen, Torben Kjersgaard

    2008-01-01

    Artiklen gennemgår pave Cølestin IIIs forhold til de nordiske kongeriger i perioden 1191-1198. Artiklen viser, at paven, som i forskningen traditionelt år har stået i skyggen af sin berømte, energiske og især: yngre efterfølger, Innocens III, har været på forkant med udviklingen i de nordiske rig...

  8. ADIÇÃO DE APARAS DE PAPEL RECICLÁVEL NA FABRICAÇÃO DE CHAPAS DE MADEIRA AGLOMERADA

    Directory of Open Access Journals (Sweden)

    Everton Hillig

    2004-05-01

    Full Text Available Neste trabalho, foram analisadas as propriedades físico-mecânicas de chapas aglomeradas produzidas com dois tipos de papel reciclável (papel jornal e offset e partículas de madeira de Pinus elliottii Engelm. As aparas de papel foram usadas no colchão em dois diferentes formatos (tiras ou moídas e em diferentes proporções: 0, 20, 40, 60 e 100% do peso seco das partículas. Utilizoaram-se 10% de adesivo à base de tanino-formaldeído na proporção 100:10. O experimento foi estabelecido segundo modelo fatorial, com três repetições. Os testes físico-mecânicos das chapas foram realizados segundo a norma ASTM D1037 (1995. Chapas aglomeradas produzidas tanto pela inclusão de papel jornal quanto offset no formato de tiras apresentaram tendência ao estouro durante a abertura dos pratos da prensa, causado pelo alto teor de umidade do colchão. A inclusão de aparas de papel prejudicaram a maioria das propriedades analisadas, fazendo com que não atendessem aos valores estabelecidos pelas normas DIN 68761 (1-1961, (3-1971 e ANSI A 208.1 (1987. A absorção d’água após 24 horas de imersão, a ligação interna e a resistência ao arrancamento de parafusos foram as propriedades mais prejudicadas. De modo geral, o uso de papel jornal se mostrou mais promissor do que o papel offset.

  9. Adição de aparas de papel reciclável na fabricação de chapas de madeira aglomerada.

    Directory of Open Access Journals (Sweden)

    Leandro Calegari

    2010-08-01

    Full Text Available Neste trabalho, foram analisadas as propriedades físico-mecânicas de chapas aglomeradas produzidas com dois tipos de papel reciclável (papel jornal e offset e partículas de madeira de Pinus elliottii Engelm. As aparas de papel foram usadas no colchão em dois diferentes formatos (tiras ou moídas e em diferentes proporções: 0, 20, 40, 60 e 100% do peso seco das partículas. Utilizoaram-se 10% de adesivo à base de tanino-formaldeído na proporção 100:10. O experimento foi estabelecido segundo modelo fatorial, com três repetições. Os testes físico-mecânicos das chapas foram realizados segundo a norma ASTM D1037 (1995. Chapas aglomeradas produzidas tanto pela inclusão de papel jornal quanto offset no formato de tiras apresentaram tendência ao estouro durante a abertura dos pratos da prensa, causado pelo alto teor de umidade do colchão. A inclusão de aparas de papel prejudicaram a maioria das propriedades analisadas, fazendo com que não atendessem aos valores estabelecidos pelas normas DIN 68761 (1-1961, (3-1971 e ANSI A 208.1 (1987. A absorção d'água após 24 horas de imersão, a ligação interna e a resistência ao arrancamento de parafusos foram as propriedades mais prejudicadas. De modo geral, o uso de papel jornal se mostrou mais promissor do que o papel offset.

  10. Cytolethal distending toxin-producing Escherichia coli strains causing severe diarrhoea in young Mexican children

    Science.gov (United States)

    Maldonado-Puga, Samantha; Huerta-Cantillo, Jazmin; Chavez-Dueñas, Lucia; Navarro-Garcia, Fernando

    2017-01-01

    Introduction. Cytolethal distending toxins (CDTs), encoded by cdt genes, have DNase activity leading to cellular and nuclear distension, resulting in irreversible cell cycle arrest and apoptosis of target cells. cdt-positive Escherichia coli strains have been isolated from children with diarrhoea. There is, however, scant information on the prevalence and clinical presentation of diarrhoeal disease caused by these strains. Furthermore, toxin production of cdt-positive strains is rarely confirmed. We report five young children with diarrhoea caused by CDT-producing E. coli in whom stools were negative for other bacterial or enteric pathogens. Case presentation. On admission to hospital, all children presented watery diarrhoea with high stool output (range 7–20 stools/24 h); five had fever of 38 °C or more and four presented vomiting. Dehydration was present in four patients, one of whom had hypovolaemic shock; one child also presented hyponatraemia and hypokalaemia. In two children, cdt-positive strains were classified as typical and atypical enteropathogenic E. coli, and the remaining three harboured cdt-positive strains that did not belong to any diarrhoeagenic pathogroup. One cdt-positive strain from each case was characterized by a CDT cytotoxic assay and a cdt type-specific PCR. All strains produced the characteristic cellular intoxication due to CDT. Two strains carried the cdt-I, one cdt-III, one cdt-IV, and one concurrently had cdt-I, cdt-II and cdt-III genes. Conclusion. Our results suggest that CDT-producing E. coli strains are an infrequent, albeit significant, cause of severe diarrhoeal illness in children. Future research should measure the true burden of cdt-positive E. coli diarrhoea among children.

  11. ESCRT-III mediated cell division in Sulfolobus acidocaldarius - a reconstitution perspective.

    Science.gov (United States)

    Härtel, Tobias; Schwille, Petra

    2014-01-01

    In the framework of synthetic biology, it has become an intriguing question what would be the minimal representation of cell division machinery. Thus, it seems appropriate to compare how cell division is realized in different microorganisms. In particular, the cell division system of Crenarchaeota lacks certain proteins found in most bacteria and Euryarchaeota, such as FtsZ, MreB or the Min system. The Sulfolobaceae family encodes functional homologs of the eukaryotic proteins vacuolar protein sorting 4 (Vps4) and endosomal sorting complex required for transport-III (ESCRT-III). ESCRT-III is essential for several eukaryotic pathways, e.g., budding of intraluminal vesicles, or cytokinesis, whereas Vps4 dissociates the ESCRT-III complex from the membrane. Cell Division A (CdvA) is required for the recruitment of crenarchaeal ESCRT-III proteins to the membrane at mid-cell. The proteins polymerize and form a smaller structure during constriction. Thus, ESCRT-III mediated cell division in Sulfolobus acidocaldarius shows functional analogies to the Z ring observed in prokaryotes like Escherichia coli, which has recently begun to be reconstituted in vitro. In this short perspective, we discuss the possibility of building such an in vitro cell division system on basis of archaeal ESCRT-III.

  12. Public Health Microbiology of Shiga Toxin-Producing Escherichia coli.

    Science.gov (United States)

    Caprioli, Alfredo; Scavia, Gaia; Morabito, Stefano

    2014-12-01

    Shiga toxin-producing Escherichia coli (STEC) strains are the only pathogenic group of E. coli that has a definite zoonotic origin, with ruminants and, in particular, cattle being recognized as the major reservoir. Most human STEC infections are food borne, but the routes of transmission include direct contact with animals and a variety of environment-related exposures. Therefore, STEC public health microbiology spans the fields of medical, veterinary, food, water, and environmental microbiology, requiring a "One Health" perspective and laboratory scientists with the ability to work effectively across disciplines. Public health microbiology laboratories play a central role in the surveillance of STEC infections, as well as in the preparedness for responding to outbreaks and in providing scientific evidence for the implementation of prevention and control measures. This article reviews (i) how the integration of surveillance of STEC infections and monitoring of these pathogens in animal reservoirs and potential food vehicles may contribute to their control; (ii) the role of reference laboratories, in both the public health and veterinary and food sectors; and (iii) the public health perspectives, including those related to regulatory issues in both the European Union and the United States.

  13. Prevalence and pathogenic potential of Escherichia coli isolates from raw milk and raw milk cheese in Egypt.

    Science.gov (United States)

    Ombarak, Rabee A; Hinenoya, Atsushi; Awasthi, Sharda Prasad; Iguchi, Atsushi; Shima, Ayaka; Elbagory, Abdel-Rahman M; Yamasaki, Shinji

    2016-03-16

    The objectives of this study were to investigate prevalence and pathogenic potential of Escherichia coli contaminating raw milk and its products in Egypt. Out of 187 dairy products including 72 raw milk samples, 55 Karish cheese and 60 Ras cheese, 222 E. coli isolates including 111, 89 and 22 were obtained from 55 raw milk samples (76.4%), 41 Karish cheese (74.5%), and 13 Ras cheese (21.7%), respectively. Isolated E. coli strains were examined for 24 representative virulence genes present in diarrheagenic E. coli (DEC) and extraintestinal pathogenic E. coli (ExPEC). Among DEC and ExPEC virulence factors, genes for enteropathogenic E. coli (eaeA, bfpA, EAF), enterohemorrhagic E. coli (stx1, stx2, eaeA), enterotoxigenic E. coli (elt, est), enteroinvasive E. coli (invE), enteroaggregative E. coli (Eagg, astA), diffusely adherent E. coli (daaD), ExPEC (cdt-I to cdt-V, cnf1, cnf2, hlyA) and putative adhesins (efa1, iha, ehaA, saa, and lpfAO113) were screened by colony hybridization assay. Out of 222 E. coli strains, 104 (46.8%) isolated from 69 (36.9%) samples carried one or more virulence genes. The most prevalent gene detected was lpfAO113 (40.5%), followed by ehaA (32.4%,), astA (3.15%,), iha (1.80%), hlyA (1.35%), stx1 (0.90%), stx2 (0.90%), eaeA (0.45%), cdt-III (0.45%) and cnf2 (0.45%). Two strains isolated from Karish cheese harbored 5 virulence genes (stx1, stx2, iha, ehaA, lpfAO113). Stx subtype was determined to be stx1 (not stx1c or stx1d) and stx2d. Indeed, expression of hemolysin A, CDT-III, CNF-II, Stx1 and Stx2d was confirmed by blood agar plate, cytotoxicity assay and Western blotting, respectively. Among the 222 E. coli strains, 54 (48.6%), 38 (42.6%) and 12 (54.7%) isolated from raw milk, Karish cheese and Ras cheese were potentially virulent, respectively. O-genotyping indicated that most of the potentially virulent E. coli isolates did not belong to clinically important O serogroups except O75, O91 and O166, which have been associated with human

  14. neonatal infections caused by escherichia coli at the national ...

    African Journals Online (AJOL)

    boaz

    Knowledge about common infections caused by E.coli as well as its antibiotics susceptibility pattern will guide ... Keywords: Neonates, E.coli Infections, Antibiotics Resistance, Abuja. ..... Resistance in E.coli 0157 Strains and the Public Health.

  15. Os radicais livres e o dano muscular produzido pelo exercício: papel dos antioxidantes

    Directory of Open Access Journals (Sweden)

    Alfredo Córdova

    2000-10-01

    Full Text Available O exercício físico intenso e contínuo é acompanhado pela produção de radicais livres, que provocam uma alteração das membranas celulares, o que causa uma lesão acompanhada por um processo inflamatório ao nível das fibras musculares. Várias causas foram sugeridas para estas alterações, entre as quais o alto grau de estresse provocado pelo exercício, alterações da microcirculação, produção de metabólitos tóxicos e depleção intramuscular dos substratos energéticos. O rápido desenvolvimento da lesão das fibras musculares e do tecido conjuntivo é acompanhado por uma disfunção e migração de componentes intracelulares para os espaços intesticial e plasmático. O dano muscular está associado com aumentos dos níveis plasmáticos de creatinoquinase (CK e de lactato desidrogenase (LDH, o que serve como indicador do aumento da permeabilidade celular resultante. A formação de radicais livres e o desencadeamento do processo de peroxidação também contribuem para o dano muscular. Embora o papel do exercício na produção da radicais livres não esteja ainda bem esclarecido, um grande número de autores sugerem que a elevação do consumo de oxigênio durante o exercício induz a produção de radicais livres e outras substâncias oxidantes. Recentemente, na literatura foi demonstrado que as vitaminas A (beta-caroteno, E (tocoferol e C (ácido ascórbico, junto com minerais como o zinco (Zn, atuam como agentes protetores antioxidantes.

  16. RESOLUCIÓN DE CONFLICTOS AL INTERIOR DEL ORGANISMO: EL PAPEL DEL SISTEMA INMUNE

    Directory of Open Access Journals (Sweden)

    Luis F. Cadavid

    2016-03-01

    Full Text Available El sistema inmune de los animales está constituido por una gran variedad de células y moléculas que colectivamente reconocen, neutralizan y eliminan potenciales agentes nocivos, tanto bióticos como abióticos. El estudio del sistema inmune ha estado tradicionalmente sesgado hacía algunas especies de importancia médica o económica, a expensas de la gran mayoría de especies que constituyen la diversidad animal. Con la actual facilidad de secuenciar genomas y transcriptomas, se ha abierto la posibilidad de estudiar los sistemas inmunes de muy variados grupos animales. Uno de estos grupos es los cnidarios, que incluye a los corales, anémonas y medusas, en los que el estudio del sistema inmune ha probado ser de gran utilidad para entender dos tipos de conflictos de relevancia en la supervivencia de estos organismos. El primero es la respuesta de los corales a enfermedades de carácter infeccioso y el segundo hace referencia a las reacciones de histocompatibilidad que median la competencia intraespecífica por el espacio habitable. Este artículo de reflexión trata en detalle el papel del sistema inmune de los cnidarios en la resolución de estos conflictos. AbstractThe immune system of animals is constituted by a large diversity of cells and molecules that collectively recognize, neutralize, and eliminate potential damaging agents, both biotic and abiotic. The study of the immune system has been traditionally biased towards some species with medical or economic importance, at the expense of the vast majority of species that constitute the animal diversity. With the current possibility of easily sequencing genomes and transcriptomes, there is an opportunity to study the immune systems of a wide variety of animal groups. One of these groups is the cnidarians, which include corals, anemones and jellyfishes, in which the study of the immune system has proved useful to understand two types of conflicts that are relevant for the survival of these

  17. Os limiares planetários, a Rio+20 e o papel do Brasil

    Directory of Open Access Journals (Sweden)

    Eduardo Viola

    Full Text Available Neste artigo consideramos os problemas ambientais globais dentro do conceito de limiares planetários, em convergência com os últimos avanços das ciências naturais. Nesse contexto, nosso objetivo é explorar o papel do Brasil na governança do espaço de operação seguro para a humanidade, avaliando como o país complementa o enorme capital ambiental físico que possui com ações políticas específicas orientadas para uma economia verde de baixo carbono (EVBC, tanto no âmbito doméstico quanto no internacional. Para atingir essa meta, em primeiro lugar discutimos conceitualmente a economia verde de baixo carbono como paradigma de desenvolvimento compatível com um espaço de operação seguro para a humanidade; em segundo lugar, analisamos a Rio+20 pelo prisma da governança global dos limiares planetários e pela atuação brasileira na Cúpula; e finalmente fazemos um diagnóstico da situação do Brasil em relação ao novo paradigma de desenvolvimento. Como conclusões da análise, destacamos a crescente distância entre a aceleração dos problemas da interdependência - especialmente a definição de um espaço seguro de operação para a humanidade - e os mecanismos globais de governança existentes, derivada de um sistema internacional bloqueado e dominado por forças conservadoras. O resultado frustrante da Rio+20 é evidência clara dessa defasagem. Nessa dinâmica, o Brasil tem o potencial para ser um ator central da governança dos limiares planetários, por seu vasto capital ambiental físico. No entanto, o mínimo avanço da EVBC no país degrada essa capacidade de agência, e o torna uma potência ambiental subdesenvolvida (underachiever environmental power.

  18. Papel de los archivos fotográficos de Derechos Humanos en la memoria colectiva

    Directory of Open Access Journals (Sweden)

    Sonia Natalia Cogollo-Ospina

    2016-01-01

    Full Text Available Se hace una revisión de investigaciones y documentos centrados en la relación establecida entre fotografía y memoria colectiva a través de los archivos fotográficos de Derechos Humanos, así como la función que cumplen los museos, centros de documentación o lugares donde se encuentran ubicados. Para la selección del material se atendió a su disponibilidad principalmente en bases de datos indexadas de reconocido prestigio: Web of Science, Scielo, EbscoHost, DOAJ y Dialnet, con descriptores como fotografía y memoria, archivo fotográfico, memoria colectiva y museos, con un corte temporal de 2006-2015. Se organiza el material en función de una serie de preguntas decisivas a la hora de abordar los problemas atinentes a la conservación de un archivo fotográfico y su papel en la memoria colectiva de poblaciones vulnerables. Los lugares de memoria reflejan una tensión entre los intereses políticos y la necesidad de las poblaciones vulnerables de manifestarse y dejar testimonio de las infracciones a los derechos humanos. La organización del material fotográfico plantea serias dificultades respecto a la conservación y clasificación con la cantidad de imágenes digitales que hoy se producen. It is made a review of researchs and documents focused in the relation between photography and collective memory trough photoarchives of human rights, and the function that have the museums, documentation centers or memory places where they are. For the material’s selection it was attended to their availability in indexed databases with prestige: Web of Science, Scielo, EbscoHost, DOAJ, and Dialnet, with descriptors as photograph and memory, photoarchive, collective memory and museums, and papers between 2006 and 2015. The material is organized in function of a series of questions about the conservation of a photoarchive and its role in collective memory of vulnerable population. The memory places reflects a tension between political interests and

  19. O Papel da escola básica como agência promotora do letramento digital

    Directory of Open Access Journals (Sweden)

    Andréa Lourdes Ribeiro

    2010-07-01

    Full Text Available RESUMO:O presente estudo procura conhecer o papel da escola básica como agência de letramento digital. O objetivo deste estudo é investigar a prática pedagógica dos professores nos laboratórios de informática, analisando a metodologia empregada e as dificuldades encontradas para ministrar aulas que empreguem os recursos da informática. Essa pesquisa toma como referencial o conceito de nova cultura, definida por Lévy (1999 com o termo cibercultura que postula a necessidade do letramento digital como uma nova habilidade que surge da interconexão mundial entre os computadores com o crescimento do ciberespaço. Os resultados da pesquisa revelaram que a escola básica pouco auxilia no desenvolvimento do letramento digital, uma vez que os professores subutilizam os laboratórios de informática e demonstram ter ainda pouca familiaridade com as tecnologias digitais e clareza quanto à metodologia utilizada para esta ferramenta. Conclui-se, então, que é preciso investir mais na formação e capacitação dos professores para que a escola básica se constitua efetivamente num local privilegiado para a formação e o desenvolvimento do letramento digital.PALAVRAS-CHAVE: Letramento Digital; Escola Básica; Formação Professores;ABSTRACT: This study seeks to understand the role of primary education as agency digital literacy. The objective of this study is to investigate the pedagogical practice of teachers in the computer labs, analyzing the methodology used and the difficulties encountered in teaching classes that employ the resources of computer. This research takes as reference the concept of new culture, defined by Levy (1999 to the term cyberculture that posits the need for literacy digital as a new ability that arises from the global interconnection between computers with the growth of cyberspace. The survey results revealed that the basic school little help in the development of digital literacy, as teachers underuse computer labs and

  20. TRATAMENTOS ALTERNATIVOS DO EFLUENTE DE UMA INDÚSTRIA DE CELULOSE BRANQUEADA E PAPEL

    Directory of Open Access Journals (Sweden)

    Celso Edmundo Bochetti Foelkel

    1998-12-01

    Full Text Available Este trabalho procurou avaliar a eficiência de tratamento floculante com sulfato de alumínio ou cloreto férrico para o efluente bruto neutralizado de uma indústria de celulose e papel. A filtração do efluente, removendo fibras, finos, macromoléculas e flocos naturais, permitiu expressivas reduções da cor aparente, cor real e DQO do mesmo. Enquanto o sulfato de alumínio mostrou excelentes resultados, o cloreto férrico não teve bom desempenho. Deve-se otimizar as concentrações, as formas de agitação e a decantação dos flocos. Para as amostras testadas, na primeira seqüência de testes, concentrações com cerca de 2500 ppm de sulfato de alumínio (com 14 moléculas de água de hidratação mostraram ótimos resultados para redução de cor aparente, cor real, DQO e cloretos. Conforme o previsto, na segunda seqüência de testes as concentrações de [Al2(SO43 . 14 H2O] diminuíram para todos os índices físico-químicos testados. A melhor concentração para pH, cor aparente e cor real foi de 1000 ppm filtrado, enquanto que para cloretos foi de 2000 ppm não filtrado, com o tempo de agitação de 1 minuto com alta velocidade e 15 minutos à baixa velocidade, com exceção da cor real que foi de 10 minutos à baixa velocidade. Como recomendação, sugere-se avaliar uma etapa seqüencial de filtração após clarifloculação/decantação. Com isso, o desempenho de um tratamento físico-químico de efluentes desse tipo será eficiente, apesar do custo envolvido na aquisição do insumo sulfato de alumínio.

  1. O papel de imunoglobulinas na nefropatia da leptospirose em suínos

    Directory of Open Access Journals (Sweden)

    Larissa M.F. Gonçalves

    2014-06-01

    Full Text Available A leptospirose é uma antropozoonose endêmica em todo o mundo, que afeta o homem e várias espécies de animais domésticos e silvestres. No início da infecção há produção de IgM para o controle da infecção e após alguns dias, IgG são produzidas e provocam lise das leptospiras circulantes. Objetivou-se neste estudo identificar depósitos de antígeno de leptospiras e imunoglobulinas no tecido renal, para avaliar o papel de imunoglobulinas na patogênese da nefropatia da leptospirose em suínos. Foram colhidas 139 amostras de sangue e rim de suínos das cidades de Teresina/PI e Timon/MA, que foram avaliadas pela SAM, imunoistoquímica e PCR. Nefrite intersticial, fibrose, vasculite, tumefação do tufo glomerular e hipercelularidade difusa foram as principais alterações histopatológicas encontradas. A imunoistoquímica detectou antígeno de leptospira em 60 suínos. Depósitos de IgG, IgM e IgA foram observados no endotélio de capilares glomerulares, dos capilares intertubulares e na cápsula de Bowman, com marcação focal, difusa, global e segmentar. A deposição de IgM e IgA foi significantemente maior nos suínos infectados. Estranhamente depósitos de IgG foi significantemente maior nos suínos não infectados, onde não havia presença de antígeno de leptospiras e nem lesão túbulo-intersticial. Concluímos que antígeno de leptospiras no rim de suínos está relacionado a depósitos de IgM e IgA mas não a depósitos de IgG.

  2. O papel do enfermeiro psiquiatra- oprimido e opressor The role of psichyatric nurse-oppressed and opressing

    Directory of Open Access Journals (Sweden)

    Carmen Lúcia Alves Filizola

    1997-08-01

    Full Text Available Este trabalho tem como objetivo analisar o papel do enfermeiro psiquiatra na assistência ao doente mental internado, entendendo esta prática não isoladamente mas enquanto prática social e histórica. A pesquisa empírica foi realizada em dois hospitais psiquiátricos, em duas etapas: observação de campo e entrevistas com os enfermeiros. Verificamos que a ênfase do papel do enfermeiro não está no relacionamento terapêutico mas em atividades administrativas e que a relação que os componentes da equipe de enfermagem mantêm com o paciente é autoritária e reproduz o autoritarismo das instituições. Portanto, o discurso da escola que define o papel do enfermeiro como terapêutico, exercido por meio do relacionamento terapêutico é de caráter ideológico, levando-nos a sugerir que as relações ensino/aprendizagem devam ser estabelecidas a partir da práxis.This work aims at analysing the role of the psychiatric nurse in assisting the hospitalized mentally ill, viewing that practice not in itself, but as a social and historical one. An empirical research was done in two psychiatric hospitals in two stages: field observations and interviews with nurses. It was checked that the emphasis the role of the psychiatic nurse is not in therapeutic relationship but in administratives activities and that the relationship the members of the nursing team have with the patient is authoritarian and reproduce the authoritarism of the institutions. Thus the precepts of school that says the chief role of the nurse is the therapeutic relationship seem to have an ideological character. That makes us suggest that the teachinglearning relationships must be established on a praxis basis.

  3. El coordinador TIC en la escuela: Análisis de su papel en procesos de innovación

    Directory of Open Access Journals (Sweden)

    Víctor Hernández Rivero

    2011-01-01

    Full Text Available Este artículo analiza el papel del coordinador de las tecnologías de la información y la comunicación (TIC en centros de educación infantil y primaria. La integración de las tecnologías informáticas en los centros supone un proceso innovador que se ha ido desarrollando en los últimos años en todos los sistemas escolares y es uno de los problemas relevantes de la investigación educativa actual. Entre los factores que inciden en los procesos de uso innovador de las TIC en las escuelas distintos estudios han destacado el papel jugado por los coordinadores de proyectos para la integración de las TIC en los centros educativos. Los objetivos del estudio que se presenta se centran en conocer las características de este rol. El diseño de investigación utilizado en el estudio es el que se denomina en la literatura especializada diseño de casos múltiples. Se estudiaron tres centros escolares a largo de los dos años de seguimiento donde se emplearon distintas técnicas de naturaleza cualitativa tales con entrevistas, análisis documental y observación. Los principales resultados destacan aspectos relacionados con el acceso a la coordinación, la formación, las funciones y actividades desempeñadas, la principal problemática con que se enfrentan y la valoración que realizan de su trabajo. Las conclusiones más destacadas apuntan al importante papel de liderazgo y apoyo de este rol en la planificación, implementación y mejora de las prácticas asociadas a la innovación con TIC en la enseñanza.

  4. El factor religioso en el conflicto de las Comunidades de Castilla (1520-1521. El papel del clero

    Directory of Open Access Journals (Sweden)

    Diago Hernando, Máximo

    2007-06-01

    Full Text Available The conflict of the «Comunidades» of Castile is analyzed in the context of the social movements, with a religious component, that took place in Europe at the end of the fifteenth century and during the first decades of the sixteenth century. It is tried to determine the role played in that conflict by the religious factor, paying attention to several questions, namely: the political role of the religious confraternities, the spread of millenaristic prophecies, or the use of preaching as an instrument of political propaganda. And, next, attention is paid to the participation in the conflict of the clerics. It is valued the role that they played in the rebel institutions, and the social profile of the clerics that joined the rebels is finally analyzed.Se analiza el conflicto de las Comunidades de Castilla en el contexto de los movimientos sociales con componente religioso que tuvieron lugar en Europa a fines del siglo XV y durante las primeras décadas del siglo XVI. Se trata de determinar el papel que en dicho conflicto desempeñó el factor religioso, prestando atención a diversas cuestiones como el papel político de las cofradías, la difusión de las profecías milenaristas o la utilización de la predicación como medio de propaganda política. Y a continuación se llama la atención sobre la participación en el conflicto de los miembros del clero, valorando el papel que desempeñaron en las instituciones comuneras, y el perfil social de los que militaron en el bando rebelde.

  5. Diarrea neonatal porcina. Aislamiento de cepas de Escherichia coli toxigénicas productoras de STa, LT y VT Neonatal diarrhoea of piglets. Isolation of toxigenic Escherichia coli strains producing STa, LT, and VT

    Directory of Open Access Journals (Sweden)

    J. ZAMORA

    1999-01-01

    Full Text Available Las cepas toxigénicas de Escherichia coli juegan un importante papel como causa de las enteritis colibacilar porcina, motivo por el cual se investigó la presencia de estos micoorganismos en fecas de cerditos con diarrea. Se aislaron 79 (27.33% cepas de Escherichia coli toxigénicas, 46 (58.2% produjeron STa, 20 (25.3% LT y 13 (16.5% VT, de las cuales 5 sintetizaban VT1, 3 VT2 y 5 expresaron simultáneamente VT1 y VT2. Una cepa que sintetizó simultáneamente VT1 y VT2 perteneció al serotipo O149: K91, K88 y 2 cepas STa+ correspondieron a los serotipos O9: K35 y la otra O149: K91Toxigenic Escherichia coli strains play an important role in the genesis of the enteric colibacillosis of swine., For this reason these mircoorganisms was investigated in faeces of piglets with diarrhoea. A total of 79 (27.3% toxigenic Escherichia coli strains were isolated, of which 46 (58.2% were STa, 20 (25.3% LT and 13 (16.5% VT. Five out of 13 VT strains synthesised VT1, 3 VT2 and 5 expressed VT1 and VT2 simultaneously. One strain that synthesised both VT1 and VT2 belonged to the serotype O149: K91; K88 and 2 strains STa+ corresponded to the serotypes O9: K35. The other one belonged to O149: K91

  6. El papel de la Ventaja Competitiva en el desarrollo económico de los países

    OpenAIRE

    Edgar Alejandro Buendía Rice

    2013-01-01

    La preocupación de los economistas por la prosperidad de las naciones ha llevado al desa - rrollo de diversas teorías que tratan de explicar por qué algunos países son más competiti - vos que otros. Este trabajo pretende hacer una breve revisión de las diferentes teorías que han estudiado las fuentes de la ventaja competitiva de las naciones, así como, examinar la importancia de ésta para el logro de la prosperidad de las mismas y el papel que juega el Estado en el desarrollo de dicha ventaja...

  7. Papel de Enfermería en el control de factores de riesgo de endoftalmitis postquirúrgica

    OpenAIRE

    Badilla Porras, Marlene Tatiana

    2017-01-01

    Nuestro objetivo es determinar el papel que desempeña enfermería en el control de factores de riesgo de endoftalmitis posterior a una cirugía ocular. El presente estudio consiste en una revisión bibliográfica, tomando en cuenta tanto fuentes de información primaria y secundaria como: libros, bases de datos, revistas biomédicas, revistas vía web a través de metabuscadores. Existen factores de riesgo individuales de los pacientes que pueden facilitar el desarrollo de infecciones postopera...

  8. El Papel Funcionamiento y la Comunicación Familiar en los Síntomas Psicosomáticos

    OpenAIRE

    Nayeli Rivero-Lazcano; Ana Martínez-Pampliega; Ioseba Iraurgi

    2011-01-01

    El objetivo de este estudio ha sido la comprobación del papel de las variables familiares (cohesión, adaptabilidad y satisfacción), en la relación de la comunicación con los síntomas psicosomáticos. Se utilizaron el modelo de los Patrones de Comunicación Familiar (Koerner y Fitzpatrick, 2004) y el Modelo Circumplejo (Olson, 2000) para estudiar la comunicación y el funcionamiento familiar, respectivamente. Para ello se contó con una muestra de 455 participantes de diversas universidades del Pa...

  9. Estudar e aprender em instituições de acolhimento : o papel de variáveis individuais e contextuais

    OpenAIRE

    Oliveira, Catarina Isabel Moura de

    2014-01-01

    A literatura tem apontado a existência de fragilidades, ao nível escolar e da aprendizagem, nos jovens em situação de acolhimento, nomeadamente, baixo rendimento escolar, elevadas taxas de retenção, absentismo, instabilidade escolar e baixo envolvimento escolar. O objetivo desta investigação foi analisar e compreender os processos de estudo e aprendizagem nas instituições de acolhimento, de crianças e jovens do 2.º e 3.º ciclo e ensino secundário, examinando o papel de variáveis individuais e...

  10. Liderança autêntica e comprometimento organizacional : o papel mediador do capital psicológico positivo

    OpenAIRE

    Rego, Paulo Jorge Ribeiro do

    2014-01-01

    Dissertação de Mestrado em Políticas de Desenvolvimento dos Recursos Humanos Este estudo tem como principa l objetivo, determinar o papel mediador do capital psicológico positivo na relação entre liderança autêntica e comprometimento organizacional. Este estudo analisa a relação entre Liderança Autêntica e Comprometimento Organizacional. Tem como objetivo principal, avaliar se o Capital Psicológico Positivo é mediador da relação entre esses dois constructos. Fora...

  11. El papel de la asertividad sexual en la sexualidad humana: una revisión sistemática

    OpenAIRE

    Pablo Santos-Iglesias; Juan Carlos Sierra

    2010-01-01

    El estudio de la asertividad sexual ha generado resultados que demuestran su importancia y su papel fundamental en la sexualidad humana. En este estudio teórico se lleva a cabo una revisión sistemática de los principales resultados obtenidos en estos estudios. Después de una búsqueda en las principales bases de datos se obtiene un total de 76 trabajos publicados entre 1980 y 2009, que demuestran que la asertividad sexual es un factor determinante tanto de la respuesta sexual como del funciona...

  12. PAPEL DE LA PROTEINA SIRT 1 EN LA DENDRITOGENESIS NEURONAL: NEUROPROTECCION CONTRA AGREGADOS DEL PEPTIDO A BETA

    OpenAIRE

    ALLARD GARROTE, CLAUDIO ANDRES; ALLARD GARROTE; CLAUDIO ANDRES

    2013-01-01

    La arborización dendrítica es requerida para la apropiada conectividad neuronal. La proteína SIRTl, es una desacetilasa de histonas dependiente de NAD+, a la cual se le asocian funciones relacionadas con el envejecimiento, longevidad y respuesta a estrés. En neuronas a la proteína SIRTl se le relaciona con la diferenciación neuronal, neurogénesis y neuroprotección. En el presente estudio, se evaluó el papel de SIRTl en el desarrollo dendrítico en cultivo de neuronas hipocampales. La sobree...

  13. En pos de los papeles perdidos del periodismo: Medios e informadores en la conflictividad democrática

    OpenAIRE

    Torrico, Erick

    2002-01-01

    El conflicto, factor consubstancial de la vida social y referente primordial de la información, la opinión y la interpretación noticiosas, ha visto modificarse su naturaleza y expresiones en la democracia. En este cuadro, el periodismo, especialmente el latinoamericano, está afectado por la erosión de sus mapas cognitivos. De lo que se trata ahora es de reinventar los papeles que tradicionalmente se asignaba a los medios y a los informadores tomando en consideración las peculiaridades de la c...

  14. El papel del capital social en la ayuda al desarrollo : un primer análisis para la OCDE

    OpenAIRE

    Portela, Marta; Neira, Isabel

    2012-01-01

    En este trabajo se analiza la relación entre el nivel de capital social de un país y su aportación en la ayuda al desarrollo. Varios trabajos han demostrado que esa ayuda sí puede influir en el nivel de capital social en un país y menos los que se han centrado en el papel de los donantes y su nivel de capital social para ver si están relacionados de alguna forma. Dado que el capital social se define como redes, confianza y normas que ayudan a la cooperación cabría esperar qu...

  15. El acolchado con papel controla la emergencia y la reproducción de Cyperus rotundus en pimiento

    OpenAIRE

    Marí León, Ana Isabel; Cirujeda Ranzenberger, Alicia; Aibar Lete, Joaquín; Zaragoza Larios, Carlos

    2013-01-01

    La juncia (Cvperus rotundus L.) es una especie de muy dificil control en cultivos hortícolas de verano. Atraviesa el acolchado de plástico pero se controla con acolchado de papel, aunque se desconoce el efecto de dicho control sobre la capacidad reproductiva de la especie. Se sembrarón un número conocido de tubérculos de juncia dentro de cajas de mallas colocadas dentro de las filas en un ensayo de campo realizado en Montañana (Zaragoza) en 2012 con diferentes materiales de aco...

  16. Las Prosperidades Restauradoras: el Papel de las Narrativas Pentecostales en las Estrategias de Gobierno del Sistema Carcelario Argentino

    OpenAIRE

    Mauricio Manchado

    2017-01-01

    RESUMEN El presente artículo se propone indagar sobre las narrativas construidas por el dispositivo religioso evangélico-pentecostal en contextos de encierro en relación al papel que ocupan con la configuración de tácticas de gubernamentalidad por parte del servicio penitenciario en Argentina. Para ello, son analizados el ordenamiento social que asumen los pabellones-iglesias, la división cosmológica espiritual/mundano en la configuración de espacios y subjetividades religiosas en prisión y, ...

  17. Enfermedad inflamatoria intestinal : papel del análisis molecular de los virus de la familia herpes sobre material tisular

    OpenAIRE

    Azueta Etxebarria, Ainara

    2014-01-01

    RESUMEN: Los virus de la familia del herpes humano afectan al hombre de manera ubicua lo que hace discutible su papel patogenético en ciertas enfermedades a pesar de demostrarse su presencia. Por otro lado, la dificultad para interpretar las técnicas de laboratorio disponibles para su detección y llegar a un diagnóstico preciso impide en la mayor parte de los casos la detección precoz de la infección y el tratamiento eficaz. Existen casos de enfermedad inflamatoria intestinal en los que...

  18. Me comunico, luego existo. El papel de la comunicación en la construcción de identidades

    OpenAIRE

    Marta Rizo García

    2005-01-01

    Este artículo plantea la necesidad de pensar la comunicación más allá de los medios masivos de difusión. En concreto, propone un debate acerca del papel de la comunicación -en su vertiente interpersonal- en la construcción de las identidades de los sujetos sociales. Luego de situar el debate teórico de la comunicación como base para la interacción social, haciendo hincapié en las aportaciones de algunas escuelas y corrientes que se han aproximado al concepto, el texto propone una aproximación...

  19. El papel de España en la derrota de la Alemania nazi durante la Segunda Guerra Mundial

    OpenAIRE

    Gordon, Bertram M.

    1999-01-01

    RESUMEN: La importancia del papel jugado por España en la derrota de la Alemania nazi muy a menudo ha sido infravalorada por los historiadores. Con su victoria sobre Francia, en junio de 1940, Alemania tuvo la oportunidad de extender su poder por España, Gibraltar, a través del Mediterráneo hacia el norte de África, por la costa occidental africana, y posiblemente hacia el Atlántico sur y hacia Sudamérica. De este modo, Gran Bretaña podría haberse visto obligada a retirarse de la guerra. Sin ...

  20. Personalidad y comportamiento suicida en adolescentes: El papel de la extraversión en la tentativa de suicidio

    Directory of Open Access Journals (Sweden)

    J. A. RUIZ

    2000-01-01

    Full Text Available En este trabajo se estudia la relación entre la extraversión y el comportamiento suicida en una muestra de 282 adolescentes de población general con ideación suicida y tentativas de suicidio. Se analiza el papel de la impulsividad y se discuten los aspectos que diferencian entre sí a los adolescentes con ideación suicida, tentativa de suicidio instrumental y tentativa real de suicidio. Finalmente se discuten las implicaciones que los resultados tienen sobre la conceptualización del fenómeno suicida en adolescentes.

  1. Conocimiento y uso de anticonceptivos entre los jóvenes mexicanos. El papel del género

    OpenAIRE

    Irene Casique

    2011-01-01

    Con base en datos provenientes de la Encuesta Nacional sobre Violencia en el Noviazgo, levantada en 2007 entre jóvenes mexicanos de 15 a 24 años de edad, se exPlora el.nivel de conocimiento y uso de anticonceptivos en el contexto de las relaciones de noviazgo. Con este análisis se pretende evidenciar el rol que desempeñan las diversas características sociodemográficas de los jóvenes en el conocimiento y uso de anticonceptivos, con partic cular atención en el papel que juega el género. En gene...

  2. Conocimiento y uso de anticonceptivos entre los jóvenes mexicanos. El papel del género

    OpenAIRE

    Irene Casique

    2011-01-01

    Con base en datos provenientes de la Encuesta Nacional sobre Violencia en el Noviazgo, levantada en 2007 entre jóvenes mexicanos de 15 a 24 años de edad, se exPlora el.nivel de conocimiento y uso de anticonceptivos en el contexto de las relaciones de noviazgo. Con este análisis se pretende evidenciar el rol que desempeñan las diversas características sociodemográficas de los jóvenes en el conocimiento y uso de anticonceptivos, con partic cular atención en el papel que juega el género. En gene...

  3. Oficina Terapêutica de Mosaico de Papel: o lugar da materialidade no campo da Terapia Ocupacional

    OpenAIRE

    2014-01-01

    Este artigo versa sobre uma experiência clínica denominada Oficina Terapêutica de Mosaico de Papel, concebida para atendimento grupal de pessoas com deficiência física, em situação de exclusão e vulnerabilidade social. Esta intervenção, ainda em curso, ocorre no âmbito da Terapia Ocupacional, orientando-se no pensamento psicanalítico, sobretudo em Winnicott. O artigo discute os temas: escolha e uso dos materiais na terapia ocupaciona...

  4. Algunos factores psicológicos y su papel en la enfermedad: una revisión

    OpenAIRE

    Rodríguez Campuzano, María de Lourdes; Universidad Nacional Autónoma de México; Frías Martínez, Laura; Universidad Nacional Autónoma de México

    2014-01-01

    Se presenta una revisión bibliográfica relacionada con los diversos factores psicológicos que, de acuerdo a distintas aproximaciones terapéuticas, influyen en el estado de salud o enfermedad de las personas. Se revisa lo que se ha planteado con respecto al papel de los factores emocionales, la personalidad, los desórdenes afectivos, el estrés, la ansiedad, el afrontamiento, las conductas de salud y la posible influencia del género. Dichos factores se exponen de acuerdo a aproximaciones como l...

  5. El papel del Polo Democrático Alternativo en las movilizaciones agrarias de la última década

    OpenAIRE

    Tache, Michael

    2014-01-01

    El interés de esta monografía es analizar el papel que han jugado las organizaciones de izquierda en las recientes movilizaciones agrarias. Para esto, se evaluará la relación entre el Polo Democrático Alternativo y el paro de los corteros de caña del 2008 y las movilizaciones lecheras en contra de los decretos que prohibían la comercialización de leche en cantina. El trabajo aborda un estudio histórico desde mitad del siglo XX que pretende evidenciar la relación gremio-gobierno y su, posterio...

  6. Papel do receptor P2X7 em modelo murino de infecção por Mycobacterium tuberculosis

    OpenAIRE

    Santos Junior,André Avelino dos

    2012-01-01

    A tuberculose (TB) continua sendo uma das principais causas de morte no mundo, devido à grande capacidade de adaptação do bacilo que pode sobreviver em diferentes condições dentro e fora do hospedeiro humano. Estudos prévios mostraram evidências de que polimorfismos no receptor purinérgico P2X7 estão associados ao aumento da suscetibilidade à TB. O presente estudo objetivou analisar o papel do receptor purinérgico P2X7, na infecção por M. tuberculosis em camundongos, e os possíveis mecanismos...

  7. El retorno a Eleusis: ¿tienen los alucinógenos un papel en terapéutica?

    OpenAIRE

    López Briz, Eduardo

    2015-01-01

    Desde los años 70, y tras la explosión de la psicodelia, el interés por el poder terapéutico de los alucinógenos (o 'psicodélicos') se había desvanecido de manera paralela a su inclusión dentro del extenso grupo de las drogas de abuso o recreativas. Recientemente han vuelto a situarse en el foco de la atención psiquiátrica por su papel en el tratamiento de la ansiedad, la depresión, las adicciones y el trastorno de estrés postraumático (TEPT).

  8. El papel de actores transnacionales a propósito de la situación derechos humanos en Venezuela

    OpenAIRE

    Falah, Natalia

    2015-01-01

    A partir de febrero de 2014, la historia de la República Bolivariana se fracturó. El descontento popular, la protesta social y las marchas pacíficas son reprimidas como consecuencia de un abuso de poder por parte del gobierno del presidente Nicolás Maduro. Las redes de información y comunicación, en los términos de Margaret Keck y Kathryn Sikkink, han jugado un papel fundamental para poner en evidencia las violaciones sistemáticas a los DD.HH., siendo estos el factor principal que vulnera la ...

  9. Responsividade materna e teoria do apego: uma discussão crítica do papel de estudos transculturais

    OpenAIRE

    Adriana F. Paes Ribas; Maria Lucia Seidl de Moura

    2004-01-01

    A responsividade materna tem sido considerada como um elemento central para a compreensão do desenvolvimento infantil e este conceito tem sido articulado com a teoria do apego. Este artigo tem como objetivo discutir criticamente o papel de estudos transculturais sobre responsividade materna, à luz da teoria do apego, a partir da revisão da literatura recente sobre o tema. Considerando a teoria do apego um referencial valioso para investigações sobre interações mãe-bebê e responsividade matern...

  10. Personalidad y comportamiento suicida en adolescentes: El papel de la extraversión en la tentativa de suicidio

    OpenAIRE

    J. A. RUIZ; Riquelme,A.; J. Buendía

    2000-01-01

    En este trabajo se estudia la relación entre la extraversión y el comportamiento suicida en una muestra de 282 adolescentes de población general con ideación suicida y tentativas de suicidio. Se analiza el papel de la impulsividad y se discuten los aspectos que diferencian entre sí a los adolescentes con ideación suicida, tentativa de suicidio instrumental y tentativa real de suicidio. Finalmente se discuten las implicaciones que los resultados tienen sobre la conceptualización del fenómeno s...

  11. Papel del directivo en la configuración de la dimensión institucional de la empresa

    OpenAIRE

    2007-01-01

    Este texto corresponde a la Clausura del Programa de Desarrollo Directivo S5 compartido entre la Universidad de Alcalá y Siemens, celebrada el día 19 de junio de 2007 en el Praninfo de la Universidad de Alacalá La dinámica de cambio afecta de manera muy relevante al papel directivo en el desarrollo de su función. Esta posee un docble carácter: por un lado debe saber descubrir, configurar y desarrollar con éxito las actividades de la empresa, los negocios, y que hay que hacerlo bien. Es lo ...

  12. Personalidad y comportamiento suicida en adolescentes: El papel de la extraversión en la tentativa de suicidio

    OpenAIRE

    Ruiz, J. A.; Riquelme,A.; J. BUENDÍA

    2000-01-01

    En este trabajo se estudia la relación entre la extraversión y el comportamiento suicida en una muestra de 282 adolescentes de población general con ideación suicida y tentativas de suicidio. Se analiza el papel de la impulsividad y se discuten los aspectos que diferencian entre sí a los adolescentes con ideación suicida, tentativa de suicidio instrumental y tentativa real de suicidio. Finalmente se discuten las implicaciones que los resultados tienen sobre la conceptualización del fenómeno s...

  13. El papel de la motivación de los asesores y profesores en el proceso de asesoramiento

    Directory of Open Access Journals (Sweden)

    Reyes Carretero

    2008-01-01

    Full Text Available El escenario de asesoramiento colaborativo es un espacio sociocultural donde lo que se hace y se dice condiciona el tipo de interacción que se va a dar entre las personas que participan así como la actividad que llevan a cabo de manera conjunta. En este artículo vamos a reflexionar sobre el papel de la motivación de los profesores y asesores para mantener el asesoramiento así como en las posibilidades que tiene el asesor para crear y mantener un contexto que resulte motivante para asesor y asesorados.

  14. Updating quasar bolometric luminosity corrections - III. [O iii] bolometric corrections

    Science.gov (United States)

    Pennell, Alison; Runnoe, Jessie C.; Brotherton, M. S.

    2017-06-01

    We present quasar bolometric corrections using the [O III] λ 5007 narrow emission line luminosity based on the detailed spectral energy distributions of 53 bright quasars at low to moderate redshift (0.0345 diversity, introduces scatter into the L_{[O III]}-Liso relationship. We found that the {[O III]} bolometric correction can be significantly improved by adding a term including the equivalent width ratio R_{Fe II} ≡ EW_{{Fe II}}/EW_{Hβ }, which is an EV1 indicator. Inclusion of R_{Fe II} in predicting Liso is significant at nearly the 3σ level and reduces the scatter and systematic offset of the luminosity residuals. Typically, {[O III]} bolometric corrections are adopted for Type 2 sources where the quasar continuum is not observed and in these cases, R_{Fe II} cannot be measured. We searched for an alternative measure of EV1 that could be measured in the optical spectra of Type 2 sources but were unable to identify one. Thus, the main contribution of this work is to present an improved {[O III]} bolometric correction based on measured bolometric luminosities and highlight the EV1 dependence of the correction in Type 1 sources.

  15. E. coli survival in waters: temperature dependence

    Science.gov (United States)

    Knowing the survival rates of water-borne Escherichia coli is important for evaluating microbial contamination and in making appropriate management decisions. E. coli survival rates are dependent on temperature; this dependency is routinely expressed using an analog of the Q10 model. This suggestion...

  16. Genes under positive selection in Escherichia coli

    DEFF Research Database (Denmark)

    Petersen, Lise; Bollback, J.P.; Dimmic, Matt

    2007-01-01

    We used a comparative genomics approach to identify genes that are under positive selection in six strains of Escherichia coli and Shigella flexneri, including five strains that are human pathogens. We find that positive selection targets a wide range of different functions in the E. coli genome...

  17. Third International E. coli genome meeting

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-12-31

    Proceedings of the Third E. Coli Genome Meeting are provided. Presentations were divided into sessions entitled (1) Large Scale Sequencing, Sequence Analysis; (2) Databases; (3) Sequence Analysis; (4) Sequence Divergence in E. coli Strains; (5) Repeated Sequences and Regulatory Motifs; (6) Mutations, Rearrangements and Stress Responses; and (7) Origins of New Genes. The document provides a collection of abstracts of oral and poster presentations.

  18. Fosfomycin Resistance in Escherichia coli, Pennsylvania, USA.

    Science.gov (United States)

    Alrowais, Hind; McElheny, Christi L; Spychala, Caressa N; Sastry, Sangeeta; Guo, Qinglan; Butt, Adeel A; Doi, Yohei

    2015-11-01

    Fosfomycin resistance in Escherichia coli is rare in the United States. An extended-spectrum β-lactamase-producing E. coli clinical strain identified in Pennsylvania, USA, showed high-level fosfomycin resistance caused by the fosA3 gene. The IncFII plasmid carrying this gene had a structure similar to those found in China, where fosfomycin resistance is commonly described.

  19. Fimbrial adhesins from extraintestinal Escherichia coli

    DEFF Research Database (Denmark)

    Klemm, Per; Hancock, Viktoria; Schembri, Mark A.

    2010-01-01

    Extraintestinal pathogenic Escherichia coli (ExPEC) represent an important subclass of E. coli that cause a wide spectrum of diseases in human and animal hosts. Fimbriae are key virulence factors of ExPEC strains. These long surface located rod-shaped organelles mediate receptor-specific attachment...

  20. Infectious endocarditis caused by Escherichia coli

    DEFF Research Database (Denmark)

    Lauridsen, Trine Kiilerich; Arpi, Magnus; Fritz-Hansen, Thomas

    2011-01-01

    Although Escherichia coli is among the most common causes of Gram-negative bacteraemia, infectious endocarditis (IE) due to this pathogen is rare. A 67-y-old male without a previous medical history presented with a new mitral regurgitation murmur and persisting E. coli bacteraemia in spite of broad...

  1. Survival of Escherichia coli in stormwater biofilters.

    Science.gov (United States)

    Chandrasena, G I; Deletic, A; McCarthy, D T

    2014-04-01

    Biofilters are widely adopted in Australia for stormwater treatment, but the reported removal of common faecal indicators (such as Escherichia coli (E. coli)) varies from net removal to net leaching. Currently, the underlying mechanisms that govern the faecal microbial removal in the biofilters are poorly understood. Therefore, it is important to study retention and subsequent survival of faecal microorganisms in the biofilters under different biofilter designs and operational characteristics. The current study investigates how E. coli survival is influenced by temperature, moisture content, sunlight exposure and presence of other microorganisms in filter media and top surface sediment. Soil samples were taken from two different biofilters to investigate E. coli survival under controlled laboratory conditions. Results revealed that the presence of other microorganisms and temperature are vital stressors which govern the survival of E. coli captured either in the top surface sediment or filter media, while sunlight exposure and moisture content are important for the survival of E. coli captured in the top surface sediment compared to that of the filter media. Moreover, increased survival was found in the filter media compared to the top sediment, and sand filter media was found be more hostile than loamy sand filter media towards E. coli survival. Results also suggest that the contribution from the tested environmental stressors on E. coli survival in biofilters will be greatly affected by the seasonality and may vary from one site to another.

  2. Genes under positive selection in Escherichia coli

    DEFF Research Database (Denmark)

    Petersen, Lise; Bollback, J.P.; Dimmic, Matt

    2007-01-01

    We used a comparative genomics approach to identify genes that are under positive selection in six strains of Escherichia coli and Shigella flexneri, including five strains that are human pathogens. We find that positive selection targets a wide range of different functions in the E. coli genome...

  3. Prevalence of Escherichia coli O157

    NARCIS (Netherlands)

    Abdissa, Rosa; Haile, Woynshet; Fite, Akafete Teklu; Beyi, Ashenafi Feyisa; Agga, Getahun E.; Edao, Bedaso Mammo; Tadesse, Fanos; Korsa, Mesula Geloye; Beyene, Takele; Beyene, Tariku Jibat; Zutter, De Lieven; Cox, Eric; Goddeeris, Bruno Maria

    2017-01-01

    Background: There is paucity of information regarding the epidemiology of Escherichia coli O157: H7 in developing countries. In this study, we investigated the occurrence of E. coli O157: H7 associated with beef cattle at processing plants and at retail shops in Ethiopia. Methods: Various samples

  4. Escherichia coli survival in waters: Temperature dependence

    Science.gov (United States)

    Knowing the survival rates of water-borne Escherichia coli is important in evaluating microbial contamination and making appropriate management decisions. E. coli survival rates are dependent on temperature, a dependency that is routinely expressed using an analogue of the Q10 mo...

  5. Strategies for Protein Overproduction in Escherichia coli.

    Science.gov (United States)

    Mott, John E.

    1984-01-01

    Examines heterologous expression in Escherichia coli and the role of regulatory sequences which control gene expression at transcription resulting in abundant production of messenger RNA and regulatory sequences in mRNA which promote efficient translation. Also examines the role of E. coli cells in stabilizing mRNA and protein that is…

  6. The unexhausted potential of E. coli.

    Science.gov (United States)

    Blount, Zachary D

    2015-03-25

    E. coli's hardiness, versatility, broad palate and ease of handling have made it the most intensively studied and best understood organism on the planet. However, research on E.coli has primarily examined it as a model organism, one that is abstracted from any natural history. But E. coli is far more than just a microbial lab rat. Rather, it is a highly diverse organism with a complex, multi-faceted niche in the wild. Recent studies of 'wild' E. coli have, for example, revealed a great deal about its presence in the environment, its diversity and genomic evolution, as well as its role in the human microbiome and disease. These findings have shed light on aspects of its biology and ecology that pose far-reaching questions and illustrate how an appreciation of E. coli's natural history can expand its value as a model organism.

  7. Guiding E.coli to nanosensors

    Science.gov (United States)

    Park, Dong-Won; So, Hye-Mi; Kim, Beom Soo; Kong, Ki-Jeong; Chang, Hyunju; Lee, Jeong-O.

    2009-03-01

    Electronic nanosensors based on nanomaterials such as carbon nanotubes and nanowires are expected to have ultimate sensitivity. However, as an inherent problem of nanosensors, they have extremely small sensor surface for reaction. Therefore, simple diffusion of target biomolecules is not enough for such nanosensors, and the problem is even more serious in the case of motile bacteria. Previously, we have shown that we could estimate the titer of E.coli with arrays of single-walled carbon nanotube field effect transistors (SWNT-FET) combined with statistical method. Still, sensitivity of our method is inferior compared with incubation method, due to the limited sensor surface area. In this work, we actively guide E.coli to the sensor surface using micro-fabricated channels. Arrow-shaped and funnel shaped microstructures were fabricated in the channel to guide E. coli to the sensor surface, and we used green fluorescent protein expressed E.coli to monitor the guiding of E.coli.

  8. ESTUDIO DEL PAPEL DEL ION COBRE SOBRE LA AGREGACION Y NEUROTOXICIDAD DEL PEPTIDO b-AMILOIDE

    OpenAIRE

    OPAZO MARTINEZ, CARLOS ROBERTO

    2002-01-01

    Las regiones del hipocampo y la corteza del cerebro de pacientes con la enfermedad de Alzheimer, presentan (i) una extensa muerte neuronal en conjunto con (ii) una acumulación de oligómeros proteicos y (iii) un incremento generalizado de los productos de daño oxidativo, cuya formación podría preceder la acumulación de los oligómeros proteicos, responsables putativos de la pérdida progresiva de las capacidades cognitivas observadas en la AD. El principal componente de los depósitos de oligómer...

  9. Influencia de la composición fibrosa y las propiedades estructurales del papel prensa en la densidad de la de impresión y la capacidad de secado.

    OpenAIRE

    Gomez Hernandez, Nuria; Molleda Clara, Maria Cristina; Villar, Juan Carlos; Quiles, R.; Ferreira, Paulo

    2010-01-01

    En los últimos años, el papel prensa se fabrica mayoritariamente con fibra reciclada y las rotativas coldset se han convertido en unas instalaciones altamente tecnológicas. En esta nueva situación, los impresores son cada vez más exigentes con el papel que emplean. La calidad de impresión en el papel prensa se evalúa mediante la densidad y la capacidad de secado. El objetivo de este trabajo es establecer cómo influyen la composición fibrosa y las características estructurales del papel e...

  10. Spectrophotometric and pH-Metric Studies of Ce(III, Dy(III, Gd(III,Yb(III and Pr(III Metal Complexes with Rifampicin

    Directory of Open Access Journals (Sweden)

    A. N. Sonar

    2011-01-01

    Full Text Available The metal-ligand and proton-ligand stability constant of Ce(III, Dy(III, Gd(III,Yb(III and Pr(III metals with substituted heterocyclic drug (Rifampicin were determined at various ionic strength by pH metric titration. NaClO4 was used to maintain ionic strength of solution. The results obtained were extrapolated to the zero ionic strength using an equation with one individual parameter. The thermodynamic stability constant of the complexes were also calculated. The formation of complexes has been studied by Job’s method. The results obtained were of stability constants by pH metric method is confirmed by Job’s method.

  11. The START III bargaining space

    Energy Technology Data Exchange (ETDEWEB)

    Karas, T.H.

    1998-08-01

    The declining state of the Russian military and precarious Russian economic condition will give the US considerable advantages at the START III bargaining table. Taking the US-RF asymmetries into account, this paper discusses a menu of START III measures the US could ask for, and measures it could offer in return, in attempting to negotiate an equitable treaty. Measures the US might seek in a START III treaty include: further reductions in deployed strategic nuclear warheads, irreversibility of reductions through warhead dismantlement; beginning to bring theater nuclear weapons under mutual control, and increased transparency into the Russian nuclear weapons complex. The US may, however, wish to apply its bargaining advantages to attempting to achieve the first steps toward two long-range goals that would enhance US security: bringing theater nuclear weapons into the US-RF arms control arena, and increasing transparency into the Russian nuclear weapons complex. In exchange for measures relating to these objectives, the US might consider offering to Russia: Further strategic weapons reductions approaching levels at which the Russians believe they could maintain a degree of parity with the US; Measures to decrease the large disparities in potential deliver-system uploading capabilities that appear likely under current START II/START III scenarios; and Financial assistance in achieving START II/START III reductions as rapidly as is technically possible.

  12. Functional cooperation of the dnaE and dnaN gene products in Escherichia coli.

    Science.gov (United States)

    Kuwabara, N; Uchida, H

    1981-09-01

    A system was designed to isolate second-site intergenic suppressors of a thermosensitive mutation of the dnaE gene of Escherichia coli. The dnaE gene codes for the alpha subunit of DNA polymerase III [McHenry, C. S. & Crow, W. (1979) J. Biol. Chem. 254, 1748-1753]. One such suppressor, named sueA77, was finely mapped and found to be located at 82 min on the E. coli chromosome, between dnaA and recF, and within the dnaN gene [Sakakibara, Y. & Mizukami, T. (1980) Mol. Gen. Genet. 178, 541-553]. The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme [Burgers, P. M. J., Kornberg, A. & Sakakibara, Y. (1981) Proc. Natl. Acad. Sci. USA 78, 5391-5395]. The sueA77 mutation was trans-dominant over its wild-type allele, and it suppressed different thermosensitive mutations of dnaE with different maximal permissive temperature. These properties were interpreted as providing genetic evidence for interaction of the dnaE and dnaN gene products in E. coli.

  13. Enterohemorrhagic Escherichia coli O157:H7 requires quorum sensing transcriptional regulators QseA and SdiA for colonization and persistence in the bovine intestinal tract

    Science.gov (United States)

    QseA and SdiA are two of several transcriptional regulators that regulate virulence gene expression of enterohemorrhagic Escherichia coli (EHEC) O157:H7 via quorum sensing (QS). QseA regulates the expression of the locus of enterocyte effacement (LEE). LEE encodes for a type III secretion (T3S) sys...

  14. Assessing the relative contributions of EspA and CsgA in cellular adherence and biofilm formation of enterohemorrhagic Escherichia coli O157:H7

    Science.gov (United States)

    In enterohemorrhagic Escherichia coli O157:H7 (O157), the locus of enterocyte effacement (LEE) encodes a type III secretion system with an extracellular filamentous structure consisting of the polymerized translocator protein EspA. The EspA filaments provide transient interactions between bacterial ...

  15. Contributions of EspA filaments and curli fimbriae in cellular adherence and biofilm formation of enterohemorrhagic Escherichia coli O157:H7

    Science.gov (United States)

    In Escherichia coli O157:H7 (O157), the filamentous structure of the type III secretion system is produced from the polymerization of the EspA protein. EspA filaments are essential for O157 adherence to epithelial cells. In previous studies, we demonstrated that O157 hha deletion mutants showed incr...

  16. El papel moderador de la autoeficacia profesional entre situaciones de acoso laboral y la salud en una muestra multiocupacional

    Directory of Open Access Journals (Sweden)

    Mariano Meseguer

    2014-05-01

    Full Text Available La autoeficacia es una competencia personal que actúa frente a los estresores incrementando o disminuyendo el malestar psicológico que estos pueden generar; por su parte el acoso psicológico o mobbing es uno de los factores psicosociales del medio laboral más dañinos para la salud de los trabajadores. Utilizando como marco de referencia el modelo teórico de demandas-recursos (JD-R model, este trabajo tiene como objetivo principal analizar el papel moderador de la autoeficacia profesional entre el acoso laboral y la salud autopercibida en una muestra multiocupacional de 772 trabajadores (379 hombres y 383 mujeres. Se administró un protocolo que contenía la escala NAQ para la medida del acoso laboral, el cuestionario de salud GHQ-12 y la subescala de autoeficacia profesional del MBI. Como resultado destaca el papel moderador de la autoeficacia sobre la salud en situaciones de acoso psicológico. Se discuten los resultados de este trabajo, las limitaciones y sus posibles implicaciones en las organizaciones.

  17. O papel do enfermeiro especialista e do mestre nos serviços de saúde é o mesmo?

    Directory of Open Access Journals (Sweden)

    Bertha Cruz Enders

    1997-03-01

    Full Text Available O propósito do estudo foi identificar o papel do enfermeiro especialista e do mestre nos serviços de saúde, a partir das expectativas dos dirigentes e dos enfermeiros nas instituições. Dirigentes de 25 serviços de saúde e 278 enfermeiros responderam um instrumento desenhado a identificar o grau de suas expectativas para com o enfermeiro com pós-graduação. Os resultados indicam que os enfermeiros e os dirigentes esperam que o enfermeiro com pós-graduação no serviço seja altamente capacitado para a assistência. Enquanto os dirigentes expressaram uma ligeira diferenciação em favor do mestre quanto ao conhecimento aprofundado da especialidade, as expectativas dos enfermeiros com relação a este são um pouco maior em todos os aspectos. Os dirigentes priorizam cursos de atualização para os enfermeiros enquanto que os enfermeiros a especialização. Conclui-se que o papel é percebido de forma igualitária para ambos os profissionais portanto, há necessidade de aprofundar as discussões sobre as funções do enfermeiro com pós-GRADUAÇÃO NOS SERVIÇOS E SOBRE OS OBJETIVOS DA PÓS-GRADUAÇÃO NA REGIÃO.

  18. O papel do enfermeiro especialista e do mestre nos serviços de saúde é o mesmo?

    Directory of Open Access Journals (Sweden)

    Bertha Cruz Enders

    Full Text Available O propósito do estudo foi identificar o papel do enfermeiro especialista e do mestre nos serviços de saúde, a partir das expectativas dos dirigentes e dos enfermeiros nas instituições. Dirigentes de 25 serviços de saúde e 278 enfermeiros responderam um instrumento desenhado a identificar o grau de suas expectativas para com o enfermeiro com pós-graduação. Os resultados indicam que os enfermeiros e os dirigentes esperam que o enfermeiro com pós-graduação no serviço seja altamente capacitado para a assistência. Enquanto os dirigentes expressaram uma ligeira diferenciação em favor do mestre quanto ao conhecimento aprofundado da especialidade, as expectativas dos enfermeiros com relação a este são um pouco maior em todos os aspectos. Os dirigentes priorizam cursos de atualização para os enfermeiros enquanto que os enfermeiros a especialização. Conclui-se que o papel é percebido de forma igualitária para ambos os profissionais portanto, há necessidade de aprofundar as discussões sobre as funções do enfermeiro com pós-GRADUAÇÃO NOS SERVIÇOS E SOBRE OS OBJETIVOS DA PÓS-GRADUAÇÃO NA REGIÃO.

  19. ANÁLISE DO DESEMPENHO ORGANIZACIONAL NO AGRONEGÓCIO BRASILEIRO: APLICANDO À AGROINDÚSTRIA DE PAPEL E CELULOSE

    Directory of Open Access Journals (Sweden)

    MARCELO ALVARO DA SILVA MACEDO

    2009-01-01

    Full Text Available El Agronegocio es uno de los sectores más importantes de la economía brasileña y su segmento industrial tiene un papel significativo. Debido a esa relevancia, cuestiones técnicas y gerenciales merecen atención especial. Este artículo procura promover una discusión sobre el desempeño organizacional de empresas brasileñas de papel y celulosa, desde informaciones contables -financieras. Por medio de la aplicación del Análisis Envolvente de Datos (DEA, se busca identificar un índice de desempeño multicriterial para cada empresa y hacer un análisis de benchmarking para mostrar lo que las empresas ineficientes necesitan hacer para hacerse eficientes. Los resultados muestran que las mejores empresas son aquéllas que mejor com - binan sus inputs (índices del tipo cuanto menor mejor a los outputs que buscan (índices del tipo cuanto mayor mejor. Además, las peores empresas son aquéllas que necesitan cambios radicales en sus niveles de input y/o output para que alcancen el desempeño máximo.

  20. O PAPEL DO IMAGINÁRIO NA FORMAÇÃO DE INFERÊNCIAS NA LEITURA

    Directory of Open Access Journals (Sweden)

    Sandra Regina Tornquist

    2008-04-01

    Full Text Available Este trabalho tem o objetivo de demonstrar que o imaginário, tomado sob o aspecto criador que lhe atribuem estudiosos como Gilbert Durand e Michel Maffesoli, entre outros, atua de forma decisiva sobre os tipos de inferências formadas a partir da leitura. Desenvolvido com base em pesquisa bibliográfica, este trabalho apresenta, inicialmente, os conceitos de imaginário adotados, bem como o material de pesquisa relativo à inferência em leitura, de maneira especial a obra Leitura: inferências e contexto sociocultural, de Regina Lúcia Péret Dell’Isola, com complementações a partir de obras de outros autores. Por fim, é analisado do papel desempenhado pelo imaginário na formação de inferências no ato de leitura. Através do estudo realizado, pode-se perceber que a inferência, criada na mente do leitor durante a leitura, é fruto do imaginário, sendo este fundamental na formação daquela, na medida em que aumenta o número de leituras possíveis. Por outro lado, a leitura também tem seu papel na constituição do imaginário, pois permite a formação de novas imagens que vão compô-lo e enriquecê-lo.

  1. Papel de gestión humana en procesos de flexibilidad numérica en organizaciones colombianas

    Directory of Open Access Journals (Sweden)

    Ninfa Del Carmen Vega-Monsalve

    2014-12-01

    Full Text Available El fenómeno de la flexibilización numérica en Colombia -referida a la autonomía de la empresa para vincular y desvincular trabajadores de acuerdo con las demandas del mercado- nace con la llegada de algunas multinacionales que implementaban, en sus países de origen, medidas de desregularización laboral. Hoy, después de 30 años, se ha convertido en una práctica común en todo tipo de empresas. Sin embargo, no existía en el país un estudio empírico que comprendiera el papel que asume gestión humana frente al escenario de flexibilización laboral numérica. Este trabajo lo hace, desde un enfoque cualitativo de estudio de caso, de tipo descriptivo a profundidad y sobre una muestra de 22 empresas de Colombia. Los resultados mostraron que gestión humana está asumiendo un papel pasivo, en un ambiente que desvirtúa los principios de la flexibilidad contractual, plantea retos para los distintos actores y contribuye a la literatura centrada en la relación entre flexibilidad numérica y gestión humana.

  2. ALERTA EMPRENDEDORA Y CONOCIMIENTO PREVIO PARA LA IDENTIFICACIÓN DE OPORTUNIDADES EMPRENDEDORAS: EL PAPEL MODERADOR DE LAS REDES SOCIALES

    Directory of Open Access Journals (Sweden)

    Ruiz-Arroyo, Matilde

    2015-01-01

    Full Text Available Este estudio contribuye a la investigación sobre la identificación de oportunidades emprendedoras, analizando el efecto moderador que el tama˜no y la fortaleza de la red de contactos del emprendedor ejercen en la relación de influencia del conocimiento previo y la alerta emprendedora sobre la identificación de oportunidades. Los resultados del análisis de regresión lineal sobre una muestra de 199 emprendedores de Andalucía muestran una influencia negativa del conocimiento previo sobre el número de oportunidades identificadas, así como un efecto moderador positivo de la fortaleza de la red en interacción con el nivel de alerta emprendedora. El presente trabajo contribuye a la literatura sobre emprendimiento proponiendo un modelo que estudia conjuntamente el papel del capital social, la alerta emprendedora y el conocimiento previo en la fase de identificación de oportunidades, y aplicando una perspectiva contingente basada en la integración del potencial papel moderador de ciertas características de las redes sociales del emprendedor.

  3. III-Nitride nanowire optoelectronics

    Science.gov (United States)

    Zhao, Songrui; Nguyen, Hieu P. T.; Kibria, Md. G.; Mi, Zetian

    2015-11-01

    Group-III nitride nanowire structures, including GaN, InN, AlN and their alloys, have been intensively studied in the past decade. Unique to this material system is that its energy bandgap can be tuned from the deep ultraviolet (~6.2 eV for AlN) to the near infrared (~0.65 eV for InN). In this article, we provide an overview on the recent progress made in III-nitride nanowire optoelectronic devices, including light emitting diodes, lasers, photodetectors, single photon sources, intraband devices, solar cells, and artificial photosynthesis. The present challenges and future prospects of III-nitride nanowire optoelectronic devices are also discussed.

  4. Papel da laparoscopia no trauma abdominal penetrante The role of laparoscopy in penetrating abdominal trauma

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Von Bahten

    2005-06-01

    Full Text Available OBJETIVO: O papel da laparoscopia na redução da taxa de laparotomias não-terapêuticas e da morbidade em pacientes vítimas de trauma abdominal penetrante tem sido amplamente discutido durante os últimos anos. O objetivo do presente estudo é relatar a experiência inicial de um hospital universitário no manejo laparoscópico de pacientes com trauma abdominal penetrante. MÉTODO: Durante um período de três anos, a laparoscopia foi realizada em 37 pacientes vítimas de trauma abdominal penetrante, hemodinamicamente estáveis. Os prontuários médicos foram revisados e os parâmetros analisados foram as indicações do procedimento, lesões associadas, necessidade de conversão, tempo de permanência hospitalar e complicações. RESULTADOS: Houve 18 (48,6% casos de laparoscopias diagnósticas (LD positivas e 19 (51,4% negativas. Dos pacientes com LD positiva, oito (44,4% foram submetidos à laparotomia exploradora com finalidade terapêutica ou para melhor delineamento da lesão. Houve 10 (55,6% LD positivas nas quais não foi realizada conversão para cirurgia aberta. Quatro pacientes apresentaram lesões reparáveis laparoscopicamente, sendo realizado hepatorrafia (n=2 e frenorrafia (n=2. Os outros seis pacientes apresentavam lesões isoladas sem sangramento ativo, e a laparotomia não-terapêutica foi evitada. Os pacientes receberam dieta no primeiro dia de pós-operatório e o tempo médio de hospitalização foi de 3,8 dias. CONCLUSÕES: Nossa experiência inicial confirma que a laparoscopia é um bom método de avaliação e tratamento no trauma penetrante. A morbidade relacionada à laparotomias desnecessárias pode ser minimizada quando o procedimento é bem indicado, e o tratamento pode ser realizado com sucesso em casos selecionados.BACKGROUND: The role of laparoscopy in reducing the rate of non-therapeutic laparotomies and the morbidity in patients sustaining penetrating abdominal trauma has been widely discussed over the last

  5. Escherichia coli transcriptional regulatory network

    Directory of Open Access Journals (Sweden)

    Agustino Martinez-Antonio

    2011-06-01

    Full Text Available Escherichia coli is the most well-know bacterial model about the function of its molecular components. In this review are presented several structural and functional aspects of their transcriptional regulatory network constituted by transcription factors and target genes. The network discussed here represent to 1531 genes and 3421 regulatory interactions. This network shows a power-law distribution with a few global regulators and most of genes poorly connected. 176 of genes in the network correspond to transcription factors, which form a sub-network of seven hierarchical layers where global regulators tend to be set in superior layers while local regulators are located in the lower ones. There is a small set of proteins know as nucleoid-associated proteins, which are in a high cellular concentrations and reshape the nucleoid structure to influence the running of global transcriptional programs, to this mode of regulation is named analog regulation. Specific signal effectors assist the activity of most of transcription factors in E. coli. These effectors switch and tune the activity of transcription factors. To this type of regulation, depending of environmental signals is named the digital-precise-regulation. The integration of regulatory programs have place in the promoter region of transcription units where it is common to observe co-regulation among global and local TFs as well as of TFs sensing exogenous and endogenous conditions. The mechanistic logic to understand the harmonious operation of regulatory programs in the network should consider the globalism of TFs, their signal perceived, coregulation, genome position, and cellular concentration. Finally, duplicated TFs and their horizontal transfer influence the evolvability of members of the network. The most duplicated and transferred TFs are located in the network periphery.

  6. Structure of Escherichia coli tryptophanase.

    Science.gov (United States)

    Ku, Shao Yang; Yip, Patrick; Howell, P Lynne

    2006-07-01

    Pyridoxal 5'-phosphate (PLP) dependent tryptophanase has been isolated from Escherichia coli and its crystal structure has been determined. The structure shares the same fold with and has similar quaternary structure to Proteus vulgaris tryptophanase and tyrosine-phenol lyase, but is found in a closed conformation when compared with these two enzymes. The tryptophanase structure, solved in its apo form, does not have covalent PLP bound in the active site, but two sulfate ions. The sulfate ions occupy the phosphoryl-binding site of PLP and the binding site of the alpha-carboxyl of the natural substrate tryptophan. One of the sulfate ions makes extensive interactions with both the transferase and PLP-binding domains of the protein and appears to be responsible for holding the enzyme in its closed conformation. Based on the sulfate density and the structure of the P. vulgaris enzyme, PLP and the substrate tryptophan were modeled into the active site. The resulting model is consistent with the roles of Arg419 in orienting the substrate to PLP and acidifying the alpha-proton of the substrate for beta-elimination, Lys269 in the formation and decomposition of the PLP quinonoid intermediate, Arg230 in orienting the substrate-PLP intermediates in the optimal conformation for catalysis, and His463 and Tyr74 in determining substrate specificity and suggests that the closed conformation observed in the structure could be induced by substrate binding and that significant conformational changes occur during catalysis. A catalytic mechanism for tryptophanase is proposed. Since E. coli tryptophanase has resisted forming diffraction-quality crystals for many years, the molecular surface of tryptophanase has been analyzed in various crystal forms and it was rationalized that strong crystal contacts occur on the flat surface of the protein and that the size of crystal contact surface seems to correlate with the diffraction quality of the crystal.

  7. Structure of Escherichia Coli Tryptophanase

    Energy Technology Data Exchange (ETDEWEB)

    Ku,S.; Yip, P.; Howell, P.

    2006-01-01

    Pyridoxal 5'-phosphate (PLP) dependent tryptophanase has been isolated from Escherichia coli and its crystal structure has been determined. The structure shares the same fold with and has similar quaternary structure to Proteus vulgaris tryptophanase and tyrosine-phenol lyase, but is found in a closed conformation when compared with these two enzymes. The tryptophanase structure, solved in its apo form, does not have covalent PLP bound in the active site, but two sulfate ions. The sulfate ions occupy the phosphoryl-binding site of PLP and the binding site of the {alpha}-carboxyl of the natural substrate tryptophan. One of the sulfate ions makes extensive interactions with both the transferase and PLP-binding domains of the protein and appears to be responsible for holding the enzyme in its closed conformation. Based on the sulfate density and the structure of the P. vulgaris enzyme, PLP and the substrate tryptophan were modeled into the active site. The resulting model is consistent with the roles of Arg419 in orienting the substrate to PLP and acidifying the {alpha}-proton of the substrate for {beta}-elimination, Lys269 in the formation and decomposition of the PLP quinonoid intermediate, Arg230 in orienting the substrate-PLP intermediates in the optimal conformation for catalysis, and His463 and Tyr74 in determining substrate specificity and suggests that the closed conformation observed in the structure could be induced by substrate binding and that significant conformational changes occur during catalysis. A catalytic mechanism for tryptophanase is proposed. Since E. coli tryptophanase has resisted forming diffraction-quality crystals for many years, the molecular surface of tryptophanase has been analyzed in various crystal forms and it was rationalized that strong crystal contacts occur on the flat surface of the protein and that the size of crystal contact surface seems to correlate with the diffraction quality of the crystal.

  8. Trigger efficiencies at BES III

    CERN Document Server

    Berger, N; Liu, Z A; Jin, D P; Xu, H; Gong, W X; Wang, K; Cao, G F

    2010-01-01

    Trigger efficiencies at BES III were determined for both the J/psi and psi' data taking of 2009. Both dedicated runs and physics datasets are used; efficiencies are presented for Bhabha-scattering events, generic hadronic decay events involving charged tracks, dimuon events and psi' -> pi+pi-J/psi, J/psi -> l+l- events (l an electron or muon). The efficiencies are found to lie well above 99% for all relevant physics cases, thus fulfilling the BES III design specifications.

  9. First Stars III Conference Summary

    CERN Document Server

    O'Shea, Brian W; Heger, Alexander; Abel, Tom

    2008-01-01

    The understanding of the formation, life, and death of Population III stars, as well as the impact that these objects had on later generations of structure formation, is one of the foremost issues in modern cosmological research and has been an active area of research during the past several years. We summarize the results presented at "First Stars III," a conference sponsored by Los Alamos National Laboratory, the Kavli Institute for Particle Astrophysics and Cosmology, and the Joint Institute for Nuclear Astrophysics. This conference, the third in a series, took place in July 2007 at the La Fonda Hotel in Santa Fe, New Mexico, U.S.A.

  10. Graphics Gems III IBM version

    CERN Document Server

    Kirk, David

    1994-01-01

    This sequel to Graphics Gems (Academic Press, 1990), and Graphics Gems II (Academic Press, 1991) is a practical collection of computer graphics programming tools and techniques. Graphics Gems III contains a larger percentage of gems related to modeling and rendering, particularly lighting and shading. This new edition also covers image processing, numerical and programming techniques, modeling and transformations, 2D and 3D geometry and algorithms,ray tracing and radiosity, rendering, and more clever new tools and tricks for graphics programming. Volume III also includes a

  11. The evolution of the Escherichia coli phylogeny.

    Science.gov (United States)

    Chaudhuri, Roy R; Henderson, Ian R

    2012-03-01

    Escherichia coli is familiar to biologists as a classical model system, ubiquitous in molecular biology laboratories around the world. Outside of the laboratory, E. coli strains exist as an almost universal component of the lower-gut flora of humans and animals. Although usually a commensal, E. coli has an alter ego as a pathogen, and is associated with diarrhoeal disease and extra-intestinal infections. The study of E. coli diversity predates the availability of molecular data, with strains initially distinguished by serotyping and metabolic profiling, and genomic diversity illustrated by DNA hybridisation. The quantitative study of E. coli diversity began with the application of multi-locus enzyme electrophoresis (MLEE), and has progressed with the accumulation of nucleotide sequence data, from single genes through multi-locus sequence typing (MLST) to whole genome sequencing. Phylogenetic methods have shed light on the processes of genomic evolution in this extraordinarily diverse species, and revealed the origins of pathogenic E. coli strains, including members of the phylogenetically indistinguishable "genus"Shigella. In May and June 2011, an outbreak of haemorrhagic uraemic syndrome in Germany was linked to a strain of enterohaemorrhagic E. coli (EHEC) O104:H4. Application of high-throughput sequencing technologies allowed the genome and origins of the outbreak strain to be characterised in real time as the outbreak was in progress.

  12. Electrochemical As(III) whole-cell based biochip sensor.

    Science.gov (United States)

    Cortés-Salazar, Fernando; Beggah, Siham; van der Meer, Jan Roelof; Girault, Hubert H

    2013-09-15

    The development of a whole-cell based sensor for arsenite detection coupling biological engineering and electrochemical techniques is presented. This strategy takes advantage of the natural Escherichia coli resistance mechanism against toxic arsenic species, such as arsenite, which consists of the selective intracellular recognition of arsenite and its pumping out from the cell. A whole-cell based biosensor can be produced by coupling the intracellular recognition of arsenite to the generation of an electrochemical signal. Hereto, E. coli was equipped with a genetic circuit in which synthesis of beta-galactosidase is under control of the arsenite-derepressable arsR-promoter. The E. coli reporter strain was filled in a microchip containing 16 independent electrochemical cells (i.e. two-electrode cell), which was then employed for analysis of tap and groundwater samples. The developed arsenic-sensitive electrochemical biochip is easy to use and outperforms state-of-the-art bacterial bioreporters assays specifically in its simplicity and response time, while keeping a very good limit of detection in tap water, i.e. 0.8ppb. Additionally, a very good linear response in the ranges of concentration tested (0.94ppb to 3.75ppb, R(2)=0.9975 and 3.75 ppb to 30ppb, R(2)=0.9991) was obtained, complying perfectly with the acceptable arsenic concentration limits defined by the World Health Organization for drinking water samples (i.e. 10ppb). Therefore, the proposed assay provides a very good alternative for the portable quantification of As (III) in water as corroborated by the analysis of natural groundwater samples from Swiss mountains, which showed a very good agreement with the results obtained by atomic absorption spectroscopy.

  13. Global transcriptional start site mapping using differential RNA sequencing reveals novel antisense RNAs in Escherichia coli.

    Science.gov (United States)

    Thomason, Maureen K; Bischler, Thorsten; Eisenbart, Sara K; Förstner, Konrad U; Zhang, Aixia; Herbig, Alexander; Nieselt, Kay; Sharma, Cynthia M; Storz, Gisela

    2015-01-01

    While the model organism Escherichia coli has been the subject of intense study for decades, the full complement of its RNAs is only now being examined. Here we describe a survey of the E. coli transcriptome carried out using a differential RNA sequencing (dRNA-seq) approach, which can distinguish between primary and processed transcripts, and an automated prediction algorithm for transcriptional start sites (TSS). With the criterion of expression under at least one of three growth conditions examined, we predicted 14,868 TSS candidates, including 5,574 internal to annotated genes (iTSS) and 5,495 TSS corresponding to potential antisense RNAs (asRNAs). We examined expression of 14 candidate asRNAs by Northern analysis using RNA from wild-type E. coli and from strains defective for RNases III and E, two RNases reported to be involved in asRNA processing. Interestingly, nine asRNAs detected as distinct bands by Northern analysis were differentially affected by the rnc and rne mutations. We also compared our asRNA candidates with previously published asRNA annotations from RNA-seq data and discuss the challenges associated with these cross-comparisons. Our global transcriptional start site map represents a valuable resource for identification of transcription start sites, promoters, and novel transcripts in E. coli and is easily accessible, together with the cDNA coverage plots, in an online genome browser.

  14. Improved pinocembrin production in Escherichia coli by engineering fatty acid synthesis.

    Science.gov (United States)

    Cao, Weijia; Ma, Weichao; Zhang, Bowen; Wang, Xin; Chen, Kequan; Li, Yan; Ouyang, Pingkai

    2016-04-01

    The development of efficient microbial processes for pinocembrin production has attracted considerable attention. However, pinocembrin biosynthetic efficiency is greatly limited by the low availability of the malonyl-CoA cofactor in Escherichia coli. Fatty acid biosynthesis is the only metabolic process in E. coli that consumes malonyl-CoA; therefore, we overexpressed the fatty acid biosynthetic pathway enzymes β-ketoacyl-ACP synthase III (FabH) and β-ketoacyl-ACP synthase II (FabF) alone and in combination, and investigated the effect on malonyl-CoA. Interestingly, overexpressing FabH, FabF or both enzymes in E. coli BL21 (DE3) decreased fatty acid synthesis and increased cellular malonyl-CoA levels 1.4-, 1.6-, and 1.2-fold, respectively. Furthermore, pinocembrin production was increased 10.6-, 31.8-, and 5.87-fold in recombinant strains overexpressing FabH, FabF and both enzymes, respectively. Overexpression of FabF, therefore, triggered the highest pinocembrin production and malonyl-CoA levels. The addition of cerulenin further increased pinocembrin production in the FabF-overexpressing strain, from 25.8 to 29.9 mg/L. These results demonstrated that overexpressing fatty acid synthases can increase malonyl-CoA availability and improve pinocembrin production in a recombinant E. coli host. This strategy may hold promise for the production of other important natural products in which cellular malonyl-CoA is rate limiting.

  15. Antibacterial Effect of Curcuma longa (Turmeric) Against Staphylococcus aureus and Escherichia coli.

    Science.gov (United States)

    Afrose, R; Saha, S K; Banu, L A; Ahmed, A U; Shahidullah, A S; Gani, A; Sultana, S; Kabir, M R; Ali, M Y

    2015-07-01

    This observational study was conducted during the period from July 2010 to June 2011 in the Department of Pharmacology in the collaboration of Department of Microbiology, Mymensingh Medical College, Mymensingh to determine the profile of antibacterial effect of Crude Turmeric paste aqueous turmeric extract, and standard antibiotic Amikacin against Staphylococcus aureus and Escherichia coli. Three separate experiments were done e.g. (Expt- I) Inhibitory effect of Crude Turmeric paste incorporated into nutrient agar (NA) media, (Expt- II) Minimum inhibitory concentration of (a) Aqueous Turmeric extract and (b) Amikacin by broth dilution technique and (Expt-III) their subculture study in nutrient agar (NA) media for confirmation of respective results of previous experiments. Inhibitory effects were observed against the growth of Staph Aureus and Esch coli at 10% and 30% respectively of Crude Turmeric paste incorporated into NA media. The broth dilution technique was followed to determine the MIC of Aqueous Turmeric extract and Amikacin. The MIC of Aqueous Turmeric extract was 800 μg/ml against Staph aureus and that against Esch coli was 2000 μg/ml and the MIC of Amikacin was 10 μg/ml for both the bacteria. The MIC of Amikacin was the lowest in comparison to MIC of Aqueous Turmeric extract for complete inhibition of growth of Staph aureus and Esch coli. The subculture study showed similar results with that of previous experiments in terms of inhibitory effects of Crude Turmeric paste and MIC of Aqueous Turmeric extract and Amikacin against all of the organisms studied.

  16. Effect of bile on growth, peritoneal absorption, and blood clearance of Escherichia coli in E coli peritonitis

    Energy Technology Data Exchange (ETDEWEB)

    Andersson, R.; Schalen, C.; Tranberg, K.G. (Department of Surgery, Lund University, Lund (Sweden))

    1991-06-01

    The effect of intraperitoneal bile on growth, peritoneal absorption, and clearance of Escherichia coli was determined in E coli peritonitis in the rat. In E coli peritonitis, intraperitoneal bacterial counts gradually decreased, whereas they increased (after 2 hours) with subsequent development of bacteremia in E coli plus bile peritonitis. After an intraperitoneal injection of labeled bacteria, blood radioactivity was only initially lower in E coli plus bile peritonitis compared with E coli peritonitis. Clearance from blood was lower in E coli plus bile peritonitis than in E coli peritonitis. Organ localization was similar in E coli peritonitis and E coli plus bile peritonitis with decreased splenic, increased pulmonary, and unchanged hepatic uptakes compared with controls. Impaired peritoneal absorption of bacteria, together with impaired local host defense, is likely to enhance the noxious effect of bile in E coli peritonitis.

  17. ESCRT-III mediated cell division in Sulfolobus acidocaldarius –A reconstitution perspective

    Directory of Open Access Journals (Sweden)

    Tobias eHärtel

    2014-06-01

    Full Text Available In the framework of Synthetic Biology, it has become an intriguing question what would be the minimal representation of cell division machinery. Thus, it seems appropriate to compare how cell division is realized in different microorganisms. In particular, the cell division system of Crenarchaeota lacks certain proteins found in most bacteria and Euryarchaeota, such as FtsZ, MreB or the Min system. The Sulfolobaceae family encodes functional homologs of the eukaryotic proteins Vps4 and ESCRT-III. ESCRT-III is essential for several eukaryotic pathways, e.g. budding of intralumenal vesicles (ILVs, or cytokinesis, whereas Vps4 dissociates the ESCRT-III complex from the membrane. CdvA (Cell Division A is required for the recruitment of crenarchaeal ESCRT-III proteins to the membrane at mid-cell. The proteins polymerize and form a smaller structure during constriction. Thus, ESCRT-III mediated cell division in S. acidocaldarius shows functional analogies to the Z ring observed in prokaryotes like E. coli, which has recently begun to be reconstituted in vitro. In this short perspective, we discuss the possibility of building such an in vitro cell division system on basis of archaeal ESCRT-III.

  18. Women living in a drug (and violence context: the maternal role Mujeres viviendo en el contexto de drogas (y violencia: el papel maternal Mulheres vivendo no contexto de drogas (e violência: papel maternal

    Directory of Open Access Journals (Sweden)

    Sueli Aparecida Frari Galera

    2005-12-01

    Full Text Available Although the drug problem is more present among men, women are an increasing group and a vulnerable subgroup, since many of them are involved in motherhood. In this paper, the partial results of an ethnographic study on maternal role-related perceptions, beliefs and attitudes are presented, as well as the context in which women with small children and are undergoing or underwent treatment for alcohol or drug addiction live. Mothers undergoing treatment participated in interviews about their maternal role and how it developed in a family context marked by violence, affective experiences and drug use.A pesar de que el problema de las drogas está más presente entre hombres, las mujeres constituyen un grupo creciente y un subgrupo vulnerable, ya que muchas entre ellas están involucradas con la maternidad. La finalidad de este trabajo es presentar los resultados parciales de un estudio etnográfico sobre las percepciones, creencias y actitudes respecto al papel maternal en el contexto de mujeres con hijos pequeños que hacen o hicieron tratamiento para el problema de la dependencia de alcohol o drogas. El rol maternal de las entrevistadas se desarrolló y se desarrolla en un contexto familiar donde los fenómenos violencia, experiencias afectivas y uso de drogas están presentes.Embora o problema das drogas esteja mais presente entre homens, as mulheres constituem um grupo crescente e um subgrupo vulnerável, já que muitas estão envolvidas com a maternidade. O objetivo deste trabalho é apresentar os resultados parciais de um estudo etnográfico sobre as percepções, crenças e atitudes em relação ao papel maternal no contexto de mulheres com filhos pequenos que fazem ou fizeram tratamento para o problema da dependência de álcool ou drogas. O papel maternal das entrevistadas desenvolveu-se e desenvolve-se em um contexto familiar marcado pela violência, experiências afetivas e uso de drogas.

  19. Diseño de un microchip de electroforesis en papel con detección electroquímica integrada

    OpenAIRE

    González López, Andrea

    2015-01-01

    Diseño de un nuevo microchip de electroforesis en plataforma papel con detección electroquímica integrada. Se ha integrado con éxito la detección electroquímica acoplando diferentes tipos de electrodos (tinta de carbono,pasta de carbono e hilos de Pt) mediante un diseño end-channel, on chip. El nuevo microchip en soporte papel es capaz de detectar un analito modelo de interés (epinefrina) con precisión así como determinar diferentes concentraciones del mismo. También se comprobó su utilidad a...

  20. El papel del voluntariado en la conservación de tortugas marinas en la península de Guanahacabibes, Cuba

    OpenAIRE

    Azanza Ricardo, Julia; Ibarra Martín, Maria Elena; Cobián Rojas, Dorka

    2014-01-01

    El presente artículo está encaminado a caracterizar las etapas de trabajo del proyecto universitario destinado a la conservación de las tortugas marinas en Cuba como una iniciativa para combinar la investigación y los esfuerzos de conservación, así como destacar el papel de los colaboradores voluntarios en estas tareas. El “Proyecto Universitario para el Estudio y Conservación de las tortugas marinas en Cuba”, que se inició en el año 1998, se ha desarrollado en tres etapas con un papel cada v...

  1. El Papel del Plan de Acción Tutorial como Herramienta en el Desarrollo Académico del Alumno

    OpenAIRE

    Balboa Ramón, Marina; Juan Vigaray, María Dolores de

    2012-01-01

    La enseñanza y el aprendizaje constituyen dos piezas claves dentro del proceso de formación de los estudiantes. El fin es lograr una formación integral que les permita un desempeño adecuado de su carrera profesional, de forma que adquieran nuevos conocimientos, siendo capaces de encontrar por sí mismos soluciones. El papel del tutor/a como guía y orientación al estudiante puede jugar un papel fundamental en este proceso. Dentro de este marco, la Universidad de Alicante creó hace siete años el...

  2. El papel de las TIC en las pr??cticas de los profesores de educaci??n primaria en Portugal: estudio exploratorio

    OpenAIRE

    Fartura, Susana; Pessoa, Teresa; Barreira, Carlos

    2014-01-01

    La constante evoluci??n de las tecnolog??as digitales ha transformado los modos de vida de las personas y, particularmente, el modo de aprendizaje. La informaci??n est?? disponible y su actualizaci??n se vuelve progresivamente m??s r??pida. La escuela cedi?? su papel de fuente ??nica de informaci??n a muchas otras formas de aprender. De este modo, el papel de la escuela tendr?? que evolucionar y proveer a los alumnos no solo de competencias de lectura, escritura y razonamiento, ...

  3. El papel de las TIC en las prácticas de los profesores de educación primaria en Portugal. Estudio exploratorio

    OpenAIRE

    Susana Fartura; Teresa Pessoa; Carlos Barreira

    2014-01-01

    La constante evolución de las tecnologías digitales ha transformado los modos de vida de las personas y, particularmente, el modo de aprendizaje. La información está disponible y su actualización se vuelve progresivamente más rápida. La escuela cedió su papel de fuente única de información a muchas otras formas de aprender. De este modo, el papel de la escuela tendrá que evolucionar y proveer a los alumnos no solo de competencias de lectura, escritura y razonamiento, sino también de competenc...

  4. El papel amortiguador de la regulación emocional sobre síntomas de ansiedad y depresión

    OpenAIRE

    2015-01-01

    La regulación emocional ha sido una dimensión clave de la inteligencia emocional cuando se evalúa como habilidad. Tradicionalmente ha sido asociada con menores síntomas de salud mental y físico. Sin embargo, aún no se ha examinado si su papel predictivo es similar para determinados síntomas con identidad conceptual propia como la ansiedad y la depresión. Objetivo: Nuestro objetivo es examinar si la regulación emocional ejercía el mismo papel amortiguador en la relación entre estrés pe...

  5. Utilidad de las muestras de sangre total desecada en papel de filtro en la detección de anticuerpos antitoxoplasma

    Directory of Open Access Journals (Sweden)

    Joaquim Gascon

    1989-04-01

    Full Text Available Se compara la técnica de Aglutinación Directa (AD utilizando muestras de sangre total desecada en papel de filtro, con la técnica de ELISA y la misma AD utilizando muestras de suero de los mismos pacientes, para la detección de anticuerpos antitoxoplasma. Los resultados muestran la validez del método de la sangre desecada en papel de filtro para la detección de anticuerpos antitoxoplasma con la técnica de AD, y se considera su utilidad en los estudios epidemiológicos de campo.

  6. Advances in genoserotyping and subtyping of Escherichia coli

    Science.gov (United States)

    E. coli plays an important role as a member of the gut microbiota; however, pathogenic strains also exist, including various diarrheagenic E. coli pathotypes and extraintestinal pathogenic E. coli that cause illness outside of the GI-tract. E. coli have traditionally been serotyped using antisera a...

  7. 21 CFR 866.3255 - Escherichia coli serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Escherichia coli serological reagents. 866.3255... coli serological reagents. (a) Identification. Escherichia coli serological reagents are devices that consist of antigens and antisera used in serological tests to identify Escherichia coli from...

  8. Organometallic neptunium(III) complexes.

    Science.gov (United States)

    Dutkiewicz, Michał S; Farnaby, Joy H; Apostolidis, Christos; Colineau, Eric; Walter, Olaf; Magnani, Nicola; Gardiner, Michael G; Love, Jason B; Kaltsoyannis, Nikolas; Caciuffo, Roberto; Arnold, Polly L

    2016-08-01

    Studies of transuranic organometallic complexes provide a particularly valuable insight into covalent contributions to the metal-ligand bonding, in which the subtle differences between the transuranium actinide ions and their lighter lanthanide counterparts are of fundamental importance for the effective remediation of nuclear waste. Unlike the organometallic chemistry of uranium, which has focused strongly on U(III) and has seen some spectacular advances, that of the transuranics is significantly technically more challenging and has remained dormant. In the case of neptunium, it is limited mainly to Np(IV). Here we report the synthesis of three new Np(III) organometallic compounds and the characterization of their molecular and electronic structures. These studies suggest that Np(III) complexes could act as single-molecule magnets, and that the lower oxidation state of Np(II) is chemically accessible. In comparison with lanthanide analogues, significant d- and f-electron contributions to key Np(III) orbitals are observed, which shows that fundamental neptunium organometallic chemistry can provide new insights into the behaviour of f-elements.

  9. Synthesis and structural characterization of hexacoordinate silicon, germanium, and titanium complexes of the E. coli siderophore enterobactin.

    Science.gov (United States)

    Baramov, Todor; Keijzer, Karlijn; Irran, Elisabeth; Mösker, Eva; Baik, Mu-Hyun; Süssmuth, Roderich

    2013-08-01

    The E. coli siderophore enterobactin, one of the strongest Fe(III) chelators known to date, is also capable of binding Si(IV) under physiological conditions. We report on the synthesis and structural characterization of the tris(catecholate) Si(IV) -enterobactin complex and its Ge(IV) and Ti(IV) analogues. Comparative structural analysis, supported by quantum-chemical calculations, reveals the correlation between the ionic radius and the structural changes in enterobactin upon complexation.

  10. Endocytosis-inducer adhesins produced by enteropathogenic serogroups of Escherichia coli participate on bacterial attachment to infant enterocytes

    Directory of Open Access Journals (Sweden)

    João Ramos Costa Andrade

    1987-03-01

    Full Text Available Enteropathogenic E. coli (EPEC infection of Hep-2 cells preoceeds through bacterial attachment to cell surface and internalization of adhered bacteria. EPEC attachment is a prerequisite for cell infection and is mediated by adhesins that recognize carbohydrate-containing receptors on cell membrane. Such endocytosis-inducer adhesins (EIA also promote EPEC binding to infant enterocytes, suggesting that EIA may have an important role on EPEC gastroenteritis.A infecção de células Hep-2 por E. coli enteropatogênicas (ECEP implica na aderência bacteriana e posterior interiorização dos microrganismos aderidos por um mecanismo de endocitose. A aderência das ECEP é pré-requisito para a infecção e é mediada por adesinas que reconhecem receptores inibidos por certas oses na membrana celular. Tais "adesinas indutoras da endocitose" (AIE também promovem a ligação bacteriana a enterócitos obtidos do intestino delgado de lactente, sugerindo que as AIE possam desempenhar algum papel nas diarréias causadas por ECEP.

  11. Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

    Science.gov (United States)

    Truffer, Frederic; Buffi, Nina; Merulla, Davide; Beggah, Siham; van Lintel, Harald; Renaud, Philippe; van der Meer, Jan Roelof; Geiser, Martial

    2014-01-01

    We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100 μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated bacteria, and a complete optical illumination/collection/detection system for automated quantitative fluorescence measurements. The device is capable of sampling water autonomously, controlling the whole measurement, storing and transmitting data over GSM networks. We demonstrate highly reproducible measurements of arsenic in drinking water at 10 and 50 μg/L within 100 and 80 min, respectively.

  12. Sialyloligosaccharide chains of laminin as an extracellular matrix target for S fimbriae of Escherichia coli.

    Science.gov (United States)

    Virkola, R; Parkkinen, J; Hacker, J; Korhonen, T K

    1993-10-01

    S fimbriae purified from recombinant Escherichia coli HB101(pANN801-13) bound strongly to extracellular matrices of cultured endothelial and epithelial cells; only poor binding was seen with the fimbriae purified from the sfaS mutant strain HB101(pANN801-1321). E. coli HB101(pANN801-13) adhered strongly to laminin immobilized on glass; no adhesion was seen to type I, III, IV, or V collagen. Strain HB101(pANN801-1321) failed to adhere to any of the target proteins. Adhesion to laminin of strain HB101(pANN801-13) was inhibited by sialyl-alpha-2,3-lactose as well as by periodate oxidation and neuraminidase treatment of laminin. In Western blotting, the purified S fimbriae recognized more strongly the A chain than the B chains of laminin.

  13. Escherichia coli DnaE Polymerase Couples Pyrophosphatase Activity to DNA Replication.

    Directory of Open Access Journals (Sweden)

    Fabio Lapenta

    Full Text Available DNA Polymerases generate pyrophosphate every time they catalyze a step of DNA elongation. This elongation reaction is generally believed as thermodynamically favoured by the hydrolysis of pyrophosphate, catalyzed by inorganic pyrophosphatases. However, the specific action of inorganic pyrophosphatases coupled to DNA replication in vivo was never demonstrated. Here we show that the Polymerase-Histidinol-Phosphatase (PHP domain of Escherichia coli DNA Polymerase III α subunit features pyrophosphatase activity. We also show that this activity is inhibited by fluoride, as commonly observed for inorganic pyrophosphatases, and we identified 3 amino acids of the PHP active site. Remarkably, E. coli cells expressing variants of these catalytic residues of α subunit feature aberrant phenotypes, poor viability, and are subject to high mutation frequencies. Our findings indicate that DNA Polymerases can couple DNA elongation and pyrophosphate hydrolysis, providing a mechanism for the control of DNA extension rate, and suggest a promising target for novel antibiotics.

  14. Protective effects of indigenous Escherichia coli against a pathogenic E. coli challenge strain in pigs.

    Science.gov (United States)

    Vahjen, W; Cuisiniere, T; Zentek, J

    2017-10-03

    To investigate the inhibitory effect of indigenous enterobacteria on pathogenic Escherichia coli, a challenge trial with postweaning pigs was conducted. A pathogenic E. coli strain was administered to all animals and their health was closely monitored thereafter. Faecal samples were taken from three healthy and three diarrhoeic animals. Samples were cultivated on MacConkey agar and isolates were subcultured. A soft agar overlay assay was used to determine the inhibitory activity of the isolates. A total of 1,173 enterobacterial isolates were screened for their ability to inhibit the E. coli challenge strain. Colony forming units of enterobacteria on MacConkey agar were not different between healthy and diarrhoeic animals in the original samples. Furthermore, numbers of isolates per animal were also not significantly different between healthy (482 isolates) and diarrhoeic animals (691 isolates). A total of 43 isolates (3.7%) with inhibitory activity against the pathogenic E. coli challenge strain were detected. All inhibitory isolates were identified as E. coli via MALDI-TOF. The isolates belonged to the phylotypes A, C and E. Many isolates (67.4%) were commensal E. coli without relevant porcine pathogenic factors, but toxin- and fimbrial genes (stx2e, fae, estIb, elt1a, fas, fan) were detected in 14 inhibitory isolates. Healthy animals showed significantly (P=0.003) more inhibitory isolates (36 of 482 isolates; 7.5%) than diseased animals (7 of 691 isolates; 1.0%). There were no significant correlations regarding phylotype or pathogenic factors between healthy and diseased animals. This study has shown that a small proportion of indigenous E. coli is able to inhibit in vitro growth of a pathogenic E. coli strain in pigs. Furthermore, healthy animals possess significantly more inhibitory E. coli strains than diarrhoeic animals. The inhibition of pathogenic E. coli by specific indigenous E. coli strains may be an underlying principle for the containment of pathogenic

  15. El calcio en los miocitos cardíacos y su papel en las miocardiopatías.

    Directory of Open Access Journals (Sweden)

    Guido Ulate Montero

    2006-01-01

    Full Text Available El papel que desempeña el calcio en los miocitos cardíacos abarca un gran número de funciones, desde su rol en el acoplamiento excitación-ccontracción hasta su papel de segundo mensajero en las diversas vías de señalización, algunas de las cuales se activan en procesos que afectan la integridad del tejido miocárdico y que tienen que ver con el crecimiento y la apoptosis de los miocitos y que al final, son los que determinan la evolución de la mayoría de las cardiomiopatías. En esta revisión se abordan los mecanismos fisiológicos en las células de músculo cardíaco en los que este ión juega un papel determinante y los cambios que se presentan en ciertas cardiopatías como la miocardiopatía arritmogénica del ventrículo derecho, las miocardiopatías asociadas a alteraciones en el receptor de rianodina y la miocardiopatía dilatada. Se revisa también la farmacodinamia de los agentes inotrópicos positivos que actúan sensibilizando los miofilamentos del sarcómero al calcio.The rol played by calcium in cardiomyocytes includes a great number of functions like the excitation-contraction coupling as well as a second messenger in diverse signaling pathways. Some of these pathways are activated in processes that affect the cardiac tissue integrity and also participate in cell growth and apoptosis, determining the poor prognosis that characterize the majority of the cardiomyopathies. In this review,the mechanisms inside the cardiomyocytes in which participates calcium and the pathophysiological changes observed in some cardiac diseases like arrhythmogenic right ventricular cardiomyopathy, catecholaminergic polymorphic ventricular tachycardia, idiopathic dilated cardiomyopathy, and others, are described. The action mechanism of positive inotropic drugs that act as sarcomere myofilaments sensitizers is also reviewed.

  16. Differentiation between Shigella, enteroinvasive Escherichia coli (EIEC) and noninvasive Escherichia coli.

    Science.gov (United States)

    van den Beld, M J C; Reubsaet, F A G

    2012-06-01

    Shigella causes bacillary dysentery and is classified into four species based on their antigen characteristics. This classification does not reflect genetic relatedness; in fact, Shigella species are so related to Escherichia coli , they should be classified as one distinctive species in the genus Escherichia. The differentiation of Shigella and E. coli is even more complicated with the description of enteroinvasive E. coli (EIEC). EIEC are strains that possess some of the biochemical characteristics of E. coli and have the ability to cause dysentery using the same method of invasion as Shigella does. Sequencing of multiple housekeeping genes indicates that EIEC is more related to Shigella than to non-invasive E. coli. Shigella and EIEC evolved from the same ancestor and form a single pathovar within E. coli. Shigella and EIEC could be separated from other E. coli by a PCR targeting the ipaH-gene; this is a multicopy gene exclusively found in all Shigella and EIEC. It is possible to differentiate Shigella and all E. coli, including EIEC, by using multiple tests, including ipaH-gene PCR, physiological and biochemical typing and serological typing. Based on literature study, a key is designed for daily use in diagnostic laboratories to identify Shigella and all E. coli.

  17. Biofuels from E. Coli: Engineering E. coli as an Electrofuels Chassis for Isooctane Production

    Energy Technology Data Exchange (ETDEWEB)

    None

    2010-07-16

    Electrofuels Project: Ginkgo Bioworks is bypassing photosynthesis and engineering E. coli to directly use carbon dioxide (CO2) to produce biofuels. E. coli doesn’t naturally metabolize CO2, but Ginkgo Bioworks is manipulating and incorporating the genes responsible for CO2 metabolism into the microorganism. By genetically modifying E. coli, Ginkgo Bioworks will enhance its rate of CO2 consumption and liquid fuel production. Ginkgo Bioworks is delivering CO2 to E. coli as formic acid, a simple industrial chemical that provides energy and CO2 to the bacterial system.

  18. antimicrobial susceptibility and plasmids from escherichia coli ...

    African Journals Online (AJOL)

    2001-10-10

    Oct 10, 2001 ... transmission to humans of E. coli containing antibiotic resistance plasmids ... resistant micro-organisms, which may in turn transfer resistance to .... cells were washed with sterile normal saline to remove leached. Я-lactamase ...

  19. ANTIMICIROBIAL SUSCEPTIBILITY PATTERNS OF Escherichia coli ...

    African Journals Online (AJOL)

    DR. AMINU

    aetiological agents of diarrhoea diseases of humans in developing ... Pathogenic organisms have developed a number of elaborate .... reported that E.coli isolated from animals haboured plasmids .... coil 0157:H7 as a model of entry of a new.

  20. First international E. coli genome meeting

    Energy Technology Data Exchange (ETDEWEB)

    1992-12-31

    This volume is a collection of abstracts of oral presentations and poster sessions of studies reported at the First International E. Coli Genome Meeting, held September 10-14, 1992 at the University of Wisconsin.

  1. First international E. coli genome meeting

    Energy Technology Data Exchange (ETDEWEB)

    1992-01-01

    This volume is a collection of abstracts of oral presentations and poster sessions of studies reported at the First International E. Coli Genome Meeting, held September 10-14, 1992 at the University of Wisconsin.

  2. Experimental induced avian E. coli salpingitis

    DEFF Research Database (Denmark)

    Olsen, Rikke Heidemann; Thøfner, Ida; Pors, Susanne Elisabeth

    2016-01-01

    Several types of Escherichia coli have been associated with extra-intestinal infections in poultry, however, they may vary significantly in their virulence potential. The aim of the present study was to investigate the virulence of five strains of E. coli obtained from different disease......) had a distinct ability to cause disease. Results of the study shows major differences in virulence of different strains of E. coli in ascending infections; however, there was no indication of tissue-specific adaptation, since strains obtained from lesions unrelated to the reproductive system were...... fully capable of causing experimental infection. In conclusion, the study provides evidence for the clinical outcome of infection with E. coli in poultry is largely influenced by the specific strain as well as individual host factors....

  3. Prevalence of diarrheagenic Escherichia coli in suckling rabbits

    OpenAIRE

    2016-01-01

    Diarrheagenic Escherichia coli (E. coli) in suckling rabbit causes collibacillosis, which is characterized by sever yellow diarrhea, poor growth and high mortalities. This study was undertaken to investigate the prevalence of diarrheagenic E. coli in suckling rabbits in Egypt. Additionally, expression of some virulence-associated genes in the isolated E. coli serotypes were examined using the polymerase chain reaction. Finally, antibiogram of the identified E. coli serotypes was also investig...

  4. Methane production from kitchen waste using Escherichia coli.

    Science.gov (United States)

    Jayalakshmi, S; Joseph, Kurian; Sukumaran, V

    2007-04-01

    Escherichia coli (E. coli) strain isolated from biogas plant sludge was examined for its ability to enhance biogas from kitchen waste during solid phase anaerobic digestion. The laboratory experiments were conducted for total solid concentrations of 20% and 22%. Kitchen waste was characterized for physico-chemical parameters and laboratory experiments were conducted with and without E. coli strain. It was found that the reactor with E. coli produced 17% more biogas than the reactors that are operated without E. coli strain.

  5. Synergistic effects in mixed Escherichia coli biofilms

    DEFF Research Database (Denmark)

    Reisner, A.; Holler, B.M.; Molin, Søren

    2006-01-01

    the strongest effects was most often linked to conjugative transmission of natural plasmids carried by the E. coli isolates (70%). Thus, the capacity of an isolate to promote the biofilm through cocultivation was (i) transferable to the K-12 strain, (ii) was linked with the acquisition of conjugation genes...... promotion in this system is not dependent on the laboratory strain and that the described model system could provide relevant insights on mechanisms of biofilm development in natural E. coli populations....

  6. Native valve Escherichia coli endocarditis following urosepsis.

    Science.gov (United States)

    Rangarajan, D; Ramakrishnan, S; Patro, K C; Devaraj, S; Krishnamurthy, V; Kothari, Y; Satyaki, N

    2013-05-01

    Gram-negative organisms are a rare cause of infective endocarditis. Escherichia coli, the most common cause of urinary tract infection and gram-negative septicemia involves endocardium rarely. In this case report, we describe infection of native mitral valve by E. coli following septicemia of urinary tract origin in a diabetic male; subsequently, he required prosthetic tissue valve replacement indicated by persistent sepsis and congestive cardiac failure.

  7. Detection of attaching and effacing virulence gene of E. coli

    Directory of Open Access Journals (Sweden)

    Maratu Soleha

    2013-07-01

    Full Text Available AbstrakLatar belakang: Bakteri Escherichia coli (E. coli ada yang telah bermutasi menjadi patogen yang menimbulkan berbagai penyakit seperti hemorrhagic colitis (HC, hemolytic uremic syndrome (HUS, sepsis, pnemonia, neonatal meningitis, dan infeksi saluran kemih. Mutasi terjadi karena bakteri ini menerima transfer gen yang virulen dari bakteri lain yang hidup di sekitarnya. E. coli yang biasanya hidup normal di dalam usus manusia telah beradaptasi sehingga bisa hidup di tanah, makanan, dan saluran kemih. Penelitian ini mendeteksi gene yang virulen pada DNA isolat E. coli. Metode: Untuk deteksi E. coli yang virulen pada penelitian ini digunakan metode Real-time PCR dengan mencocokkan hasil sekuensing dengan sekuens E. coli virulen yang telah di publikasikan sebagai rujukan. Hasil: Sekuens RT PCR menggambarkan DNA gen eae pada BLAST mempunyai kesesuaian dengan rujukan segmen E. coli yang virulen. Dari sampel yang berasal dari E. coli di sekitar perairan lingkungan didapatkan gen Eae sebagai gen yang menyebabkan E. coli menjadi virulen sebesar 7,3%. Kesimpulan: E. coli yang virulen ditemukan pada sampel E. coli yang berasal dari perairan lingkungan dengan metode realtime PCR. (Health Science Indones 2013;1:41-6 Kata kunci: gen virulen E. coli, real-time PCR, perairan lingkunganAbstractBackground: Escherichia coli(E. coli bacteria have developed into pathogenic bacteria that caused diseases such as hemorrhagic colitis (HC, hemolytic uremic syndrome (HUS, sepsis, pneumonia, neonatal meningitis, and urinary tract infections. Pathogenic E. coli have acquired pathogenic/virulence genes from other bacteria in their environment. E. coli that normally lived in the human gut had adapted to other niches such as soil, food and the urinary tract. This study investigated the presence of pathogenic E. coli from water samples by examining E. coli virulence genes present in E. coli genomes of water sourced isolates. Methods:This study used Real-time PCR to detect

  8. Detection of attaching and effacing virulence gene of E. coli

    Directory of Open Access Journals (Sweden)

    Maratu Soleha

    2013-07-01

    Full Text Available AbstrakLatar belakang: Bakteri Escherichia coli (E. coli ada yang telah bermutasi menjadi patogen yang menimbulkan berbagai penyakit seperti hemorrhagic colitis (HC, hemolytic uremic syndrome (HUS, sepsis, pnemonia, neonatal meningitis, dan infeksi saluran kemih. Mutasi terjadi karena bakteri ini menerima transfer gen yang virulen dari bakteri lain yang hidup di sekitarnya. E. coli yang biasanya hidup normal di dalam usus manusia telah beradaptasi sehingga bisa hidup di tanah, makanan, dan saluran kemih. Penelitian ini mendeteksi gene yang virulen pada DNA isolat E. coli. Metode: Untuk deteksi E. coli yang virulen pada penelitian ini digunakan metode Real-time PCR dengan mencocokkan hasil sekuensing dengan sekuens E. coli virulen yang telah di publikasikan sebagai rujukan. Hasil: Sekuens RT PCR menggambarkan DNA gen eae pada BLAST mempunyai kesesuaian dengan rujukan segmen E. coli yang virulen. Dari sampel yang berasal dari E. coli di sekitar perairan lingkungan didapatkan gen Eae sebagai gen yang menyebabkan E. coli menjadi virulen sebesar 7,3%. Kesimpulan: E. coli yang virulen ditemukan pada sampel E. coli yang berasal dari perairan lingkungan dengan metode realtime PCR. (Health Science Indones 2013;1:41-6 Kata kunci: gen virulen E. coli, real-time PCR, perairan lingkunganAbstractBackground: Escherichia coli(E. coli bacteria have developed into pathogenic bacteria that caused diseases such as hemorrhagic colitis (HC, hemolytic uremic syndrome (HUS, sepsis, pneumonia, neonatal meningitis, and urinary tract infections. Pathogenic E. coli have acquired pathogenic/virulence genes from other bacteria in their environment. E. coli that normally lived in the human gut had adapted to other niches such as soil, food and the urinary tract. This study investigated the presence of pathogenic E. coli from water samples by examining E. coli virulence genes present in E. coli genomes of water sourced isolates. Methods:This study used Real-time PCR to detect

  9. Siderophore production by uropathogenic Escherichia coli

    Directory of Open Access Journals (Sweden)

    Vagrali Manjula

    2009-01-01

    Full Text Available Urinary tract infection (UTI is one of the most frequently encountered problems in ambulatory medicine. The present study was designed to determine siderophore production as the urovirulence factor of Escherichia coli isolated from the patients of UTI. A total of 160 strains of E. coli isolated from urine of patients with clinically diagnosed UTI were included in the study and 50 fecal isolates of E. coli, siderophore production was seen in 156 (97.5%. In 50 fecal isolates, siderophore production was seen in 2 (4%. Siderophore production has been shown to be more frequent in E. coli from patients with UTI, than in fecal isolates. The results suggest that siderophore production positive strains can be considered as UPEC. Thus, although a great deal has been learned regarding E. coli virulence mechanisms in UTI, much remains to be learned and the practical application of our growing understanding of E. coli virulence factors to the prevention and treatment of UTI has to be continued.

  10. E. coli on the move

    Science.gov (United States)

    Calne, S.

    2012-04-01

    Lynn Grove High School in Great Yarmouth, UK has been awarded a Royal Society partnership grant. Lynn Grove pupils aged between 11 and 16 years will carry out an investigation collaborating with scientists at the John Innes Centre in Norwich, UK to investigate the distribution of E.coli and other coliform bacteria within a school. The information will be used as an evidence base to educate pupils about the transmission of microbes and about methods of control. Through this work pupils will gain an appreciation of the diversity of microbial biochemistry and the chemistry behind chromogenic detection methodologies for specific bacterial enzymes. Inferences from the use of diagnostic selective media will be confirmed by carrying out DNA isolation and PCR to identify the genes responsible for the biochemical reactions. PCR will also be used to identify species of coliforms by reference to genomic sequence databases. These techniques will allow pupils to look into an unseen world in a way which has direct relevance to their everyday lives. Furthermore this partnership study will demonstrate to pupils that solving scientific questions requires the integration of a variety of scientific disciplines. The project will run from January 2012 until June 2012. We will present our preliminary results from the investigation and outline our future plans.

  11. Enterohemorrhagic E. coli (EHEC pathogenesis

    Directory of Open Access Journals (Sweden)

    Vanessa eSperandio

    2012-07-01

    Full Text Available Enterohemorrhagic Escherichia coli (EHEC serotype O157:H7 is a human pathogen responsible for outbreaks of bloody diarrhea and hemolytic uremic syndrome worldwide. Conventional antimicrobials trigger an SOS response in EHEC that promotes the release of the potent Shiga toxin that is responsible for much of the morbidity and mortality associated with EHEC infection. Cattle are a natural reservoir of EHEC, and approximately 75% of EHEC outbreaks are linked to the consumption of contaminated bovine-derived products. This review will discuss how EHEC causes disease in humans but is asymptomatic in adult ruminants. It will also analyze factors utilized by EHEC as it travels through the bovine gastrointestinal tract that allow for its survival through the acidic environment of the distal stomachs, and for its ultimate colonization in the recto-anal junction. Understanding the factors crucial for EHEC survival and colonization in cattle will aid in the development of alternative strategies to prevent EHEC shedding into the environment and consequent human infection.

  12. La Gramática del Papel y la Referencia: una aproximación al modelo

    Directory of Open Access Journals (Sweden)

    Carlos González Vergara

    2006-12-01

    Full Text Available Este texto es una presentación de los principios fundamentales de la Gramática del Papel y la Referencia, teoría lingüística de orientación funcionalista que ofrece un modelo que relaciona de manera integral los aspectos semánticos, sintácticos y pragmáticos que rigen las lenguas humanas. Los postulados se exponen con especial atención a su aplicación en el estudio de las estructurasgramaticales del español. (This text presents the basic principles of Role and Reference Grammar, a linguistic theory that embraces the semantic, syntactic and pragmatics aspects that rule human languages. This principles are explained specially in relation to Spanish grammatical structures.

  13. O papel do princípio aristotélico na teoria da justiça de Rawls

    Directory of Open Access Journals (Sweden)

    Denis Coitinho Silveira

    2010-12-01

    Full Text Available http://dx.doi.org/10.5007/1677-2954.2008v7n2p201O objetivo do presente artigo é  estabelecer algumas considerações sobre o papel do princípio aristotélico no interiror da teoria da jsutiça como eqüidade de John Rawls formulado em A Theory of Justice e não modificado em Political Liberalism e Justice as Fainess: A Restatement, a fim de verificar uma convergência entre um modelo deontológico e precedimental com um modelo teleológico e substancial de justiça, identificando um compatibilismo entre justo e bem em sua teoria ética.

  14. O papel das interacções fármaco-membrana nos efeitos cardiovasculares de ß-bloqueadores

    OpenAIRE

    Carneiro, Cláudia Alexandra Vieira

    2011-01-01

    Mestrado em Controlo de Qualidade MSc in Quality Control As interacções fármaco-membrana desempenham um importante papel para: o desenvolvimento de novos fármacos; o desenvolvimento de sistemas de libertação controlada de fármacos; a compreensão dos efeitos terapêuticos e tóxicos; o estabelecimento de relações estrutura-actividade; a compreensão dos efeitos ecotoxicológicos dos fármacos; a compreensão do mecanismo de resistência a múltiplos fármacos; o controlo da inibição enzimática; e...

  15. El papel de las instituciones educativas públicas en la eliminación de la pobreza

    Directory of Open Access Journals (Sweden)

    Blanca Zuluaga

    2005-01-01

    Full Text Available Este trabajo presenta un conjunto de propuestas que contribuirían a fortalecer el papel de las instituciones educativas en la eliminación de la pobreza. Se trata de un conjunto de acciones por parte del Gobierno y las instituciones educativas que podría hacer más eficiente el gasto público en educación. El documento es el resultado del trabajo de campo realizado en las instituciones educativas del Distrito de Agua blanca en Cali, de la revisión de la literatura nacional e internacional y del análisis de información complementaria efectuados por el equipo de investigación de la Universidad Icesi sobre el problema de la pobreza.

  16. Envolvimento em atos infracionais e com drogas como possibilidades para lidar com o papel de filho parental

    Directory of Open Access Journals (Sweden)

    Maria Aparecida Penso

    2004-01-01

    Full Text Available Este estudo busca compreender a contribuição da dinâmica familiar no processo de construção identitária de adolescentes que cometeram atos infracionais e são usuários de drogas, vivendo em situação de pobreza e exclusão social. Seu referencial teórico é a Teoria Sistêmica. Utiliza-se da metodologia qualitativa de pesquisa-intervenção com dez adolescentes em medida sócio-educativa de semiliberdade e suas famílias. Os resultados apontam para a função do uso de drogas e do cometimento de atos infracionais na adolescência, como uma das saídas encontradas pelo adolescente para lidar com o papel de filho parental, permitindo a resolução da crise identitária.

  17. Feminización de la migración y papel de las mujeres en el hecho migratorio

    Directory of Open Access Journals (Sweden)

    Sara Victoria Alvarado

    2012-05-01

    Full Text Available Las transformaciones del mercado de trabajo, a escala global, han determinadocambios en la demanda del tipo de fuerza de trabajo requerida desde los centros de generaciónde tal demanda. La feminización del boom migratorio Quito-Madrid 1998-2001 instaló nuevassubjetividades ancladas en cambios en las relaciones de poder entre hombre-mujer (concebidosdesde la categoría rol de género en el contexto de la dinámica familiar y en torno de un radicalviraje de las valoraciones acerca de la importancia de separarse o no de los hijos e hijas y dela familia. Se considera que los campos conceptuales de género, poder y subjetividad son los quemayores posibilidades analíticas y explicativas ofrecen para la comprensión del papel de la mujerprogenitora-madre en el hecho migratorio.

  18. El papel de la interpolación y la predicción en el cálculo

    OpenAIRE

    2006-01-01

    En esta investigación se desarrolla un análisis epistemológico para mostrar el papel de la interpolación y la predicción en el Cálculo con énfasis en la matematización del movimiento, el binomio de Newton y la serie de Taylor. Nuestro análisis está matizado por la aproximación socoiepistemológica entendida como epistemología de prácticas sociales (Cantoral, 2001), con la intención de diseñar una situación del movimiento uniformemente acelerado de un cuerpo.

  19. Papel patogénico de la interleuquina-10 en el síndrome de Sjögren

    Directory of Open Access Journals (Sweden)

    Juan Manuel Anaya

    2001-04-01

    Full Text Available La interleuquina (IL-10 es una citoquina con múltiples actividades
    sobre linfocitos B y T. Fisiológicamente es considerada como inmunosupresora; sin embargo, en patología este papel no ha sido confirmado en todos los casos (1. El síndrome de Sjögren primario (SSp es una enfermedad autoinmune caracterizada por la hiperactividad de los linfocitos B y un infiltrado mononuclear en las glándulas exocrinas responsable de su hipofunción (2. En el presente estudio se investigó simultáneamente el nivel sérico de la IL-10 en pacientes con SSp y su correlación con los títulos de autoanticuerpos, manifestaciones clínicas, actividad de la enfermedad y polimorfismo del gen de la IL-10.

  20. O papel da fisioterapia respiratória na bronquiolite viral aguda = Role of chest physiotherapy in acute viral bronchiolitis

    Directory of Open Access Journals (Sweden)

    Luisi, Fernanda

    2008-01-01

    Conclusões: mesmo que não existem evidências diretas, a fisioterapia respiratória tem sido utilizada na bronquiolite aguda com objetivo de desobstrução, higiene brônquica, prevenção de atelectasias e recrutamento alveolar, podendo contribuir para diminuição da resistência das vias aéreas, promover melhor ventilação-perfusão e diminuir o trabalho ventilatório pela remoção do excesso de muco. São necessários estudos clínicos randomizados, controlados e cegados, que avaliem as técnicas mais modernas, para que se possa definir o papel da fisioterapia respiratória no tratamento da bronquiolite viral aguda

  1. Escritores combativos. Reflexiones sobre el papel del intelectual en la construcción democrática

    Directory of Open Access Journals (Sweden)

    Ana Amélia de Moura Cavalcante de Melo

    2011-01-01

    Full Text Available En los años 30 y 40 del siglo XX, es posible identifi car una fuerte y expresiva preocupación entre los intelectuales brasileños respecto a su actuación en la vida política del país. Los escritores toman como tema central el drama social y político, así como los ensayos histórico-sociológicos. Este artículo busca refl exionar sobre como los intelectuales reunidos en la Asociación Brasileña de Escritores perciben la realidad nacional, cuales son los análisis que elaboran y de qué manera piensan su papel en la sociedad.

  2. Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata

    Energy Technology Data Exchange (ETDEWEB)

    Morita, Hiroyuki [Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511 (Japan); Kondo, Shin; Kato, Ryohei [Innovation Center Yokohama, Mitsubishi Chemical Corporation, 1000 Kamoshida, Aoba, Yokohama, Kanagawa 227-8502 (Japan); Wanibuchi, Kiyofumi; Noguchi, Hiroshi [School of Pharmaceutical Sciences, University of Shizuoka and the COE21 Program, Shizuoka 422-8526 (Japan); Sugio, Shigetoshi, E-mail: sugio.shigetoshi@mw.m-kagaku.co.jp [Innovation Center Yokohama, Mitsubishi Chemical Corporation, 1000 Kamoshida, Aoba, Yokohama, Kanagawa 227-8502 (Japan); Abe, Ikuro, E-mail: sugio.shigetoshi@mw.m-kagaku.co.jp [School of Pharmaceutical Sciences, University of Shizuoka and the COE21 Program, Shizuoka 422-8526 (Japan); PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan); Kohno, Toshiyuki, E-mail: sugio.shigetoshi@mw.m-kagaku.co.jp [Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511 (Japan)

    2007-07-01

    An acridone-producing novel type III polyketide synthase from H. serrata has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.0 Å. Polyketide synthase 1 (PKS1) from Huperzia serrata is a plant-specific type III polyketide synthase that shows an unusually versatile catalytic potential, producing various aromatic tetraketides, including chalcones, benzophenones, phlorogulucinols and acridones. Recombinant H. serrata PKS1 expressed in Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group I222 or I2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 73.3, b = 85.0, c = 137.7 Å, α = β = γ = 90.0°. Diffraction data were collected to 2.0 Å resolution using synchrotron radiation at BL24XU of SPring-8.

  3. Expression, refolding and spectroscopic characterization of fibronectin type III (FnIII)-homology domains derived from human fibronectin leucine rich transmembrane protein (FLRT)-1,-2, and-3

    DEFF Research Database (Denmark)

    Yang, Lila; Falkesgaard, Maria Hansen; Thulstrup, Peter Waaben

    2017-01-01

    The fibronectin leucine rich transmembrane (FLRT) protein family consists in humans of 3 proteins, FLRT1, -2, and -3. The FLRT proteins contain two extracellular domains separated by an unstructured linker. The most membrane distal part is a leucine rich repeat (LRR) domain responsible for both cis......- and trans-interactions, whereas the membrane proximal part is a fibronectin type III (FnIII) domain responsible for a cis-interaction with members of the fibroblast growth factor receptor 1 (FGFR1) family, which results in FGFR tyrosine kinase activation. Whereas the structures of FLRT LRR domains from...... in inclusion bodies in Escherichia coli. His-tags permitted affinity purification of the domains, which subsequently were refolded on a Ni-NTA agarose column by reducing the concentration of urea. The refolding was confirmed by circular dichroism (CD) and 1H-NMR. By thermal unfolding experiments we show...

  4. Circulación intestinal: Su organización, control y papel en el paciente crítico.

    Directory of Open Access Journals (Sweden)

    Raúl Narváez Sánchez

    2009-11-01

    Full Text Available El intestino ha ganado un espacio fundamental en el campo de la monitorización y del manejo de pacientes en unidades de cuidados intensivos, porque este órgano puede desempeñar tanto un papel protector, como un papel promotor en la insuficiencia multiorgánica del enfermo crítico. Por tal motivo desde hace 20 años, la fisiología intestinal ha dado base a un área de investigación floreciente, en la carrera por validar métodos mínimamente invasivos que permitan efectuar un diagnóstico temprano y una monitorización efectiva del choque (p.e., tonometría, espectrometría de impedancia y oximetría de pulso endoluminal. Con el fin de mejorar la comprensión del funcionamiento y del control de la circulación sanguínea en el tubo digestivo, sobre todo en el estómago y en el intestino del ser humano, se presenta una revisión actualizada que parte de un recuento elemental de la distribución anatómica e histológica comparada de los vasos sanguíneos del abdomen y de la pared intestinal. También se revisa de modo breve la biofísica aplicable. Por último, se estudian los mecanismos reguladores del flujo sanguíneo en el tubo digestivo, con actualizaciones sobre la información clásica.

  5. El Papel de los Organismos Supervisores de las Bolsas en el Proceso de Armonización Contable Internacional

    Directory of Open Access Journals (Sweden)

    Juan Luis Gandía Cabedo

    1998-06-01

    Full Text Available El objetivo de este artículo consiste en poner de manifiesto el papel que en los últimos años están desempeñando los organismos supervisores de los mercados de valores en el proceso de armonización contable internacional. Así, las mayores necesidades de financiación de ciertas empresas que podríamos denominar "transnacionales" y la creciente globalización del negocio bursátil, acrecientan el interés de los mercados de capitales porque las sociedades cotizadas depositen información financiera que sea comparable para sus usuarios internacionales. En este sentido, analizamos la viabilidad del acuerdo suscrito entre la 10SCO y el IASC en julio de 1995, teniendo en cuenta la estrategia propia adoptada por los mercados de capitales estadounidenses y el papel que como organismo normalizador desempeña en aquel país el FASB. Todo ello supone cambios sustanciales en el futuro de la armonización contable internacional. The purpose of this article is to analyze the role player by supervisory organizations of the stock markets in the process of international accounting harmonization. Thus, the increasing needs for financing experience by international corporations and the growing globalization of the stock market, increase the inte¬rest of the capital markets because the companies listing deposit financial information that it will be comparable for their international users. In this sense, we analyze the viability of the agreement subscribed among the IOSCO and the IASC in July of 1995, taking into account the own strategy adopted by American stock markets and the paper that as standard organization performs in that country the FASE. All this supposes changes essential in the future of the international accounting harmonization.

  6. O Papel da ONU e do Banco Mundial na Consolidação do Campo Internacional de Desenvolvimento

    Directory of Open Access Journals (Sweden)

    Fernanda Cimini Salles

    2015-12-01

    Full Text Available ResumoO artigo tem por objetivo resgatar o processo histórico de construção do modus operandi das práticas de desenvolvimento, destacando o papel das organizações internacionais nesse processo e como elas mobilizavam seus recursos tangíveis e intangíveis para a conformação de uma agenda legítima de intervenção social. A análise terá como foco as dinâmicas políticas que sucederam a Comissão Pearson, conferência internacional realizada em 1969, que teve como objetivo repensar e propor diretrizes internacionais para o desenvolvimento. O ponto crítico deste momento será o reconhecimento, por parte do Banco Mundial e da ONU, de que a cooperação internacional para o desenvolvimento mobilizava diferentes projetos de coordenação societária que tinham como pano de fundo o problema da desigualdade internacional. A partir da análise dos documentos e discursos da época, é possível perceber que, embora a ONU e o Banco Mundial compartilhassem a percepção de que a ajuda internacional ainda teria um papel a cumprir, esses organismos ofereciam agendas distintas para solucionar o problema do subdesenvolvimento. Com isso, buscar-se-á identificar o projeto que sai vitorioso desse embate e nos fornece elementos para a compreensão da sobrevivência e ampliação da agenda de desenvolvimento no pós-Guerra Fria.

  7. The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates.

    Science.gov (United States)

    Rasko, David A; Rosovitz, M J; Myers, Garry S A; Mongodin, Emmanuel F; Fricke, W Florian; Gajer, Pawel; Crabtree, Jonathan; Sebaihia, Mohammed; Thomson, Nicholas R; Chaudhuri, Roy; Henderson, Ian R; Sperandio, Vanessa; Ravel, Jacques

    2008-10-01

    Whole-genome sequencing has been skewed toward bacterial pathogens as a consequence of the prioritization of medical and veterinary diseases. However, it is becoming clear that in order to accurately measure genetic variation within and between pathogenic groups, multiple isolates, as well as commensal species, must be sequenced. This study examined the pangenomic content of Escherichia coli. Six distinct E. coli pathovars can be distinguished using molecular or phenotypic markers, but only two of the six pathovars have been subjected to any genome sequencing previously. Thus, this report provides a seminal description of the genomic contents and unique features of three unsequenced pathovars, enterotoxigenic E. coli, enteropathogenic E. coli, and enteroaggregative E. coli. We also determined the first genome sequence of a human commensal E. coli isolate, E. coli HS, which will undoubtedly provide a new baseline from which workers can examine the evolution of pathogenic E. coli. Comparison of 17 E. coli genomes, 8 of which are new, resulted in identification of approximately 2,200 genes conserved in all isolates. We were also able to identify genes that were isolate and pathovar specific. Fewer pathovar-specific genes were identified than anticipated, suggesting that each isolate may have independently developed virulence capabilities. Pangenome calculations indicate that E. coli genomic diversity represents an open pangenome model containing a reservoir of more than 13,000 genes, many of which may be uncharacterized but important virulence factors. This comparative study of the species E. coli, while descriptive, should provide the basis for future functional work on this important group of pathogens.

  8. Uma Relação a Três: O Papel Político e Sociológico do Terceiro no Tratamento dos Conflitos

    Directory of Open Access Journals (Sweden)

    Fabiana Marion Spengler

    Full Text Available RESUMO O presente artigo objetiva discutir o papel político e sociológico do “terceiro” (incluído e excluído na administração, gestão, tratamento e resolução dos conflitos, tomando como base, especialmente, as teorias de Carl Schmitt (papel político e de Georg Simmel (papel sociológico. Posteriormente, a análise se voltará para a necessidade do “terceiro” na construção de um “pluriverso” político que fuja da dualidade universalista e binária na qual se encontram envolvidos os atuais conflitos sociais. Para cumprir o objetivo proposto utilizou-se do método de abordagem hermenêutico no qual se pretende partir da análise de categorias consideradas fundamentais para o desenvolvimento do tema (como o conflito social, e o desenvolvimento do papel do terceiro no tratamento de tais conflitos para então enfrentar o problema propriamente dito. Como método de procedimento utilizou-se o comparativo e como técnica aplicou-se a pesquisa bibliográfica baseada em documentação indireta que serviu de base teórica para o desenvolvimento do estudo.

  9. Determinación de Anticuerpos IgM contra Virus dengue partir de sangre absorbida en papel filtro: Un método alternativo y sencillo

    Directory of Open Access Journals (Sweden)

    María García M

    2000-01-01

    Full Text Available Objetivo: Se propone como alternativa la obtención de muestras de sangre total en papel filtro, para la determinación de anticuerpos IgM contra dengue, por ser un método de recolección sencillo y no requerir de muchos cuidados en el envío al laboratorio. Materiales y métodos: De 100 pacientes con diagnóstico clínico de dengue clásico, se obtuvieron en forma simultánea, muestras de suero en tubos al vacío y de sangre total en papel filtro. Ambas muestras fueron evaluadas con el método serológico ELISA Captura de IgM a los 30 días de obtenida la muestra. Resultados: De las 100 muestras 25 fueron positivas y 75 negativas en suero, y 24 positivas y 74 negativas en papel filtro. Se obtuvo una concordancia por índice Kappa de 0,97, sensibilidad y especificidad de 96,0% y 98,0% respectivamente; el valor predictivo positivo fue 96,0%, y valor predictivo negativo fue 98,0%. Conclusión: Se evidencia muy buena sensibilidad, especificidad y concordancia en la determinación de IgM contra dengue, al utilizar papel filtro en la obtención de muestra de sangre.

  10. Determinación de IgG y anticuerpos totales contra el Virus Dengue, en muestras obtenidas en papel filtro

    Directory of Open Access Journals (Sweden)

    María García

    1997-01-01

    Full Text Available Se propone como alternativa para la determinación de anticuerpos contra el virus del dengue, el uso de muestras de sangre total obtenidos en papel filtro, por ser un método sencillo y no requerir de muchos cuidados en el envío al laboratorio. Se evaluaron 100 muestras de suero obtenidas en tubos al vacío y sangre total en papel filtro respectivamente, de pacientes con diagnóstico clínico de Dengue clásico. Ambas muestras fueron evaluadas con los métodos serológicos de ELISA Indirecta para detectar IgG e Inhibición de la Hemoaglutinación para detectar anticuerpos totales. Por ELISA indirecta se obtuvo un total de 51 positivos y 49 negativos en suero; 45 positivos y 48 negativos en papel filtro, con una concordancia por índice Kappa de 0.92, sensibilidad de 88% y especificidad de 98%. Por Inhibición de la Hemaglutinación se obtuvo un total de 45 positivos y 55 negativos en suero; 40 positivos y 50 negativos en papel filtro, con una concordancia por índice Kappa de 0,88, sensibilidad de 87% y especificidad de 91%.

  11. The role of beta-ketoacyl-acyl carrier protein synthase III in the condensation steps of fatty acid biosynthesis in sunflower.

    Science.gov (United States)

    González-Mellado, Damián; von Wettstein-Knowles, Penny; Garcés, Rafael; Martínez-Force, Enrique

    2010-05-01

    The beta-ketoacyl-acyl carrier protein synthase III (KAS III; EC 2.3.1.180) is a condensing enzyme catalyzing the initial step of fatty acid biosynthesis using acetyl-CoA as primer. To determine the mechanisms involved in the biosynthesis of fatty acids in sunflower (Helianthus annuus L.) developing seeds, a cDNA coding for HaKAS III (EF514400) was isolated, cloned and sequenced. Its protein sequence is as much as 72% identical to other KAS III-like ones such as those from Perilla frutescens, Jatropha curcas, Ricinus communis or Cuphea hookeriana. Phylogenetic study of the HaKAS III homologous proteins infers its origin from cyanobacterial ancestors. A genomic DNA gel blot analysis revealed that HaKAS III is a single copy gene. Expression levels of this gene, examined by Q-PCR, revealed higher levels in developing seeds storing oil than in leaves, stems, roots or seedling cotyledons. Heterologous expression of HaKAS III in Escherichia coli altered their fatty acid content and composition implying an interaction of HaKAS III with the bacterial FAS complex. Testing purified HaKAS III recombinant protein by adding to a reconstituted E. coli FAS system lacking condensation activity revealed a novel substrate specificity. In contrast to all hitherto characterized plant KAS IIIs, the activities of which are limited to the first cycles of intraplastidial fatty acid biosynthesis yielding C6 chains, HaKAS III participates in at least four cycles resulting in C10 chains.

  12. Mechatronic systems and materials III

    CERN Document Server

    Gosiewski, Zdzislaw

    2009-01-01

    This very interesting volume is divided into 24 sections; each of which covers, in detail, one aspect of the subject-matter: I. Industrial robots; II. Microrobotics; III. Mobile robots; IV. Teleoperation, telerobotics, teleoperated semi-autonomous systems; V. Sensors and actuators in mechatronics; VI. Control of mechatronic systems; VII. Analysis of vibration and deformation; VIII. Optimization, optimal design; IX. Integrated diagnostics; X. Failure analysis; XI. Tribology in mechatronic systems; XII. Analysis of signals; XIII. Measurement techniques; XIV. Multifunctional and smart materials;

  13. The Negotiation of Basel III

    DEFF Research Database (Denmark)

    Just, Sine Nørholm

    2015-01-01

    While the Basel Accords of 1988 and 2004 (Basel I and Basel II) ostensibly set out to regulate bank risk at the international level, they were effectively in the grip of neoliberal beliefs in the self-regulating potential of free markets. In 2009–2011, the Basel Accords were revised once more wit...... agency, the empirical argument is substantiated through textual–intertextual analysis of the rhetorical circulation of affective signs in the Basel III negotiations....

  14. Bacterial characteristics of importance for recurrent urinary tract infections caused by Escherichia coli.

    Science.gov (United States)

    Ejrnæs, Karen

    2011-04-01

    quiescent intracellular reservoirs that may represent stable reservoirs for RUTIs. The IBC pathogenic cycle has not been studied in humans; however, recently exfoliated IBCs were detected in urine from women with acute uncomplicated cystitis supporting the presence of the IBC pathway and occurrence of an intracellular bacterial niche in some women with UTI. Based on a triplex PCR E. coli can be divided into four main phylogenetic groups (A, B1, B2 and D). Phylogenetic group B2 was the most predominant group among the primary infecting E. coli followed by group D, A and B1. The majority of the tested 29 VFGs were associated with phylogenetic group B2, whereas only a few VFGs were more broadly distributed among the phylogenetic groups (Paper III). Primary infecting E. coli causing persistence or relapse of the infection were associated with phylogenetic group B2, whereas primary infecting E. coli followed by cure or reinfection were associated with group D (Paper II). Phylogenetic group B2 was associated with susceptibility to many of the tested antimicrobials, whereas group A was associated with resistance to many of these antimicrobials and multidrug resistant (MDR) strains, and group D with MDR strains. Phylogenetic group A and D were associated with carriage of IncH and IncI plasmids, respectively. Resistance patterns or plasmid profiles of the primary infecting E. coli were not associated with outcome during follow-up (cure, persistence, reinfection or relapse) (Paper II). Resistance to ampicillin, sulfamethizole, streptomycin and tetracycline was associated with a lower prevalence of some VFGs (sfa/focDE, agn43bCFT073, chuA, iroN, cnf1, hlyD, ibeA, malX, usp) and higher prevalence of other VFGs (afa/draBC, agn43aCFT073, iha, iutA, sat) but the aggregate VFG score did not differ among the resistant and susceptible strains of these antimicrobials (Paper III). Primary infecting E. coli causing persistence or relapse showed to have a higher biofilm formation capacity in

  15. Antimicrobial resistance in equine faecal Escherichia coli isolates from North West England

    Directory of Open Access Journals (Sweden)

    Williams Nicola J

    2010-04-01

    Full Text Available Abstract Background Escherichia coli isolates of equine faecal origin were investigated for antibiotic resistance, resistance genes and their ability to perform horizontal transfer. Methods In total, 264 faecal samples were collected from 138 horses in hospital and community livery premises in northwest England, yielding 296 resistant E. coli isolates. Isolates were tested for susceptibility to antimicrobial drugs by disc diffusion and agar dilution methods in order to determine minimum inhibitory concentrations (MIC. PCR amplification was used to detect genes conferring resistance to: ampicillin (TEM and SHV beta-lactamase, chloramphenicol (catI, catII, catIII and cml, tetracycline (tetA, tetB, tetC, tetD, tet E and tetG, and trimethoprim (dfrA1, dfrA9, dfrA12, dfrA13, dfr7, and dfr17. Results The proportion of antibiotic resistant isolates, and multidrug resistant isolates (MDR was significantly higher in hospital samples compared to livery samples (MDR: 48% of hospital isolates; 12% of livery isolates, p dfr, TEM beta-lactamase, tet and cat, conferring resistance to trimethoprim, ampicillin, tetracycline and chloramphenicol, respectively. Within each antimicrobial resistance group, these genes occurred at frequencies of 93% (260/279, 91%, 86.8% and 73.5%, respectively; with 115/296 (38.8% found to be MDR isolates. Conjugation experiments were performed on selected isolates and MDR phenotypes were readily transferred. Conclusions Our findings demonstrate that E. coli of equine faecal origin are commonly resistant to antibiotics used in human and veterinary medicine. Furthermore, our results suggest that most antibiotic resistance observed in equine E. coli is encoded by well-known and well-characterized resistant genes common to E. coli from man and domestic animals. These data support the ongoing concern about antimicrobial resistance, MDR, antimicrobial use in veterinary medicine and the zoonotic risk that horses could potentially pose to

  16. Molecular and antimicrobial susceptibility analyses distinguish clinical from bovine Escherichia coli O157 strains.

    Science.gov (United States)

    Vidovic, Sinisa; Tsoi, Sarah; Medihala, Prabhakara; Liu, Juxin; Wylie, John L; Levett, Paul N; Korber, Darren R

    2013-07-01

    A population-based study combining (i) antimicrobial, (ii) genetic, and (iii) virulence analyses with molecular evolutionary analyses revealed segregative characteristics distinguishing human clinical and bovine Escherichia coli O157 strains from western Canada. Human (n = 50) and bovine (n = 50) strains of E. coli O157 were collected from Saskatchewan and Manitoba in 2006 and were analyzed by using the six-marker lineage-specific polymorphism assay (LSPA6), antimicrobial susceptibility analysis, the colicin assay, plasmid and virulence profiling including the eae, ehxA, espA, iha, stx1, stx2, stx2c, stx2d, stx2d-activatable, stx2e, and stx2f virulence-associated genes, and structure analyses. Multivariate logistic regression and Fisher's exact test strongly suggested that antimicrobial susceptibility was the most distinctive characteristic (P = 0.00487) associated with human strains. Among all genetic, virulence, and antimicrobial determinants, resistance to tetracycline (P coli O157 strains. Among 11 virulence-associated genes, stx2c showed the strongest association with E. coli O157 strains of bovine origin. LSPA6 genotyping showed the dominance of the lineage I genotype among clinical (90%) and bovine (70%) strains, indicating the importance of lineage I in O157 epidemiology and ecology. Population structure analysis revealed that the more-diverse bovine strains came from a unique group of strains characterized by a high degree of antimicrobial resistance and high frequencies of lineage II genotypes and stx2c variants. These findings imply that antimicrobial resistance generated among bovine strains of E. coli O157 has a large impact on the population of this human pathogen.

  17. Escherichia coli Population Structure and Antibiotic Resistance at a Buffalo/Cattle Interface in Southern Africa.

    Science.gov (United States)

    Mercat, Mathilde; Clermont, Olivier; Massot, Méril; Ruppe, Etienne; de Garine-Wichatitsky, Michel; Miguel, Eve; Valls Fox, Hugo; Cornelis, Daniel; Andremont, Antoine; Denamur, Erick; Caron, Alexandre

    2015-12-28

    At a human/livestock/wildlife interface, Escherichia coli populations were used to assess the risk of bacterial and antibiotic resistance dissemination between hosts. We used phenotypic and genotypic characterization techniques to describe the structure and the level of antibiotic resistance of E. coli commensal populations and the resistant Enterobacteriaceae carriage of sympatric African buffalo (Syncerus caffer caffer) and cattle populations characterized by their contact patterns in the southern part of Hwange ecosystem in Zimbabwe. Our results (i) confirmed our assumption that buffalo and cattle share similar phylogroup profiles, dominated by B1 (44.5%) and E (29.0%) phylogroups, with some variability in A phylogroup presence (from 1.9 to 12%); (ii) identified a significant gradient of antibiotic resistance from isolated buffalo to buffalo in contact with cattle and cattle populations expressed as the Murray score among Enterobacteriaceae (0.146, 0.258, and 0.340, respectively) and as the presence of tetracycline-, trimethoprim-, and amoxicillin-resistant subdominant E. coli strains (0, 5.7, and 38%, respectively); (iii) evidenced the dissemination of tetracycline, trimethoprim, and amoxicillin resistance genes (tet, dfrA, and blaTEM-1) in 26 isolated subdominant E. coli strains between nearby buffalo and cattle populations, that led us (iv) to hypothesize the role of the human/animal interface in the dissemination of genetic material from human to cattle and toward wildlife. The study of antibiotic resistance dissemination in multihost systems and at anthropized/natural interface is necessary to better understand and mitigate its multiple threats. These results also contribute to attempts aiming at using E. coli as a tool for the identification of pathogen transmission pathway in multihost systems.

  18. Organometallic neptunium(III) complexes

    Science.gov (United States)

    Dutkiewicz, Michał S.; Farnaby, Joy H.; Apostolidis, Christos; Colineau, Eric; Walter, Olaf; Magnani, Nicola; Gardiner, Michael G.; Love, Jason B.; Kaltsoyannis, Nikolas; Caciuffo, Roberto; Arnold, Polly L.

    2016-08-01

    Studies of transuranic organometallic complexes provide a particularly valuable insight into covalent contributions to the metal-ligand bonding, in which the subtle differences between the transuranium actinide ions and their lighter lanthanide counterparts are of fundamental importance for the effective remediation of nuclear waste. Unlike the organometallic chemistry of uranium, which has focused strongly on UIII and has seen some spectacular advances, that of the transuranics is significantly technically more challenging and has remained dormant. In the case of neptunium, it is limited mainly to NpIV. Here we report the synthesis of three new NpIII organometallic compounds and the characterization of their molecular and electronic structures. These studies suggest that NpIII complexes could act as single-molecule magnets, and that the lower oxidation state of NpII is chemically accessible. In comparison with lanthanide analogues, significant d- and f-electron contributions to key NpIII orbitals are observed, which shows that fundamental neptunium organometallic chemistry can provide new insights into the behaviour of f-elements.

  19. Antithrombin III and the nephrotic syndrome.

    Science.gov (United States)

    Jørgensen, K A; Stoffersen, E

    1979-05-01

    Plasma and urinary antithrombin III (AT-III) was measured in 15 cases of nephrotic syndrome. Plasma AT-III correlated well with serum albumin, but poorly with proteinuria, whereas urinary AT-III correlated well to proteinuria. The plasma AT-III level had a mean similar to 25 healthy controls, but the range was significantly wider. A case with nephrotic syndrome and left renal vein thrombosis is reported. The urinary output of AT-III rose and the plasma level fell with the activity of the disease. Although AT-III and albumin have similar molecule weight, their renal clearance was found to be different. It is suggested that urinary loss of AT-III plays a role in the hypercoagulable state sometimes found in the nephrotic syndrome.

  20. The Many Worlds of Hugh Everett III

    CERN Document Server

    ,

    2011-01-01

    A review of Peter Byrne's biography of Hugh Everett III, "The Many Worlds of Hugh Everett III: Multiple Universes, Mutual Assured Destruction, and the Meltdown of a Nuclear Family", (Oxford University Press, 2010).

  1. The asymptomatic bacteriuria Escherichia coli strain 83972 outcompetes uropathogenic E. coli strains in human urine

    DEFF Research Database (Denmark)

    Hancock, Viktoria; Ulett, G.C.; Schembri, M.A.

    2006-01-01

    Escherichia coli is the most common organism associated with asymptomatic bacteriuria (ABU). In contrast to uropathogenic E. coli (UPEC), which causes symptomatic urinary tract infections (UTI), very little is known about the mechanisms by which these strains colonize the human urinary tract...

  2. Identification and Prevalence of Escherichia coli and Escherichia coli O157: H7 in Foods

    Directory of Open Access Journals (Sweden)

    Ancuta Mihaela Rotar

    2013-11-01

    Full Text Available The objective of this study is to investigate the incidence of Escherichia coli in animal and non-animal foods, and mainly the incidence of the serotype O157: H7 producing verotoxin. The presence of common Escherichia coli and Escherichia coli O157: H7 in various foods (of animal and non animal origin was performed in Transylvania area. We analyzed a total of one hundred forty-one samples of minced meat, one hundred twenty-six samples of meat , twenty six samples of meat products, five samples of alcoholic beverages, three samples of seafood, one hundred samples of cheese from pasteurized milk, seventeen samples of butter, four samples of vegetables and one sample of milk powder, using the standard cultural method and Vidas Eco method for E. coli O157: H7 strains. E. coli was identified in 50 samples of minced meat, 55 samples of meat prepared, 4 samples of meat products, 2 samples of alcoholic beverages, 25 samples of cheese from pasteurized milk, 6 samples of butter and 1 sample of vegetables. In this study were not been identified any foods contaminated with the E. coli O157: H7 serotype. The results of this reasearch have demostrated that E. coli wich represents a hygienic indicator of recent food contamination, can be destroyed with heat treatment and hygienic handling of foods. Our country over the years has been among the few countries where the incidence of the E. coli O157: H7 serotype has been minimal.

  3. The asymptomatic bacteriuria Escherichia coli strain 83972 outcompetes uropathogenic E. coli strains in human urine

    DEFF Research Database (Denmark)

    Hancock, Viktoria; Ulett, G.C.; Schembri, M.A.

    2006-01-01

    Escherichia coli is the most common organism associated with asymptomatic bacteriuria (ABU). In contrast to uropathogenic E. coli (UPEC), which causes symptomatic urinary tract infections (UTI), very little is known about the mechanisms by which these strains colonize the human urinary tract....... The prototype ABU E. coli strain 83972 was originally isolated from a girl who had carried it asymptomatically for 3 years. Deliberate colonization of UTI-susceptible individuals with E. coli 83972 has been used successfully as an alternative approach for the treatment of patients who are refractory...... to conventional therapy. Colonization with strain 83972 appears to prevent infection with UPEC strains in such patients despite the fact that this strain is unable to express the primary adhesins involved in UTI, viz. P and type 1 fimbriae. Here we investigated the growth characteristics of E. coli 83972 in human...

  4. Analysis of unassisted translesion replication by the DNA polymerase III holoenzyme.

    Science.gov (United States)

    Tomer, G; Livneh, Z

    1999-05-01

    DNA damage-induced mutations are formed when damaged nucleotides present in single-stranded DNA are replicated. We have developed a new method for the preparation of gapped plasmids containing site-specific damaged nucleotides, as model DNA substrates for translesion replication. Using these substrates, we show that the DNA polymerase III holoenzyme from Escherichia coli can bypass a synthetic abasic site analogue with high efficiency (30% bypass in 16 min), unassisted by other proteins. The theta and tau subunits of the polymerase were not essential for bypass. No bypass was observed when the enzyme was assayed on a synthetic 60-mer oligonucleotide carrying the same lesion, and bypass on a linear gapped plasmid was 3-4-fold slower than on a circular gapped plasmid. There was no difference in the bypass when standing-start and running-start replication were compared. A comparison of translesion replication by DNA polymerase I, DNA polymerase II, the DNA polymerase III core, and the DNA polymerase III holoenzyme clearly showed that the DNA polymerase III holoenzyme was by far the most effective in performing translesion replication. This was not only due to the high processivity of the pol III holoenzyme, because increasing the processivity of pol II by adding the gamma complex and beta subunit, did not increase bypass. These results support the model that SOS regulation was imposed on a fundamentally constitutive translesion replication reaction to achieve tight control of mutagenesis.

  5. Transtornos alimentares: o papel dos aspectos culturais no mundo contemporâneo Transtornos alimentarios: el papel de los aspectos culturales en el mundo contemporáneo Eating disorders: the role of cultural aspects in the contemporary world

    Directory of Open Access Journals (Sweden)

    Leticia Langlois Oliveira

    2010-09-01

    Full Text Available Os transtornos alimentares são patologias caracterizadas primordialmente pelo medo mórbido de engordar. Vários são os fatores envolvidos tanto na etiologia quanto na manutenção e na gravidade dessas doenças, principalmente fatores individuais, familiares e culturais. Em relação a estes últimos, salienta-se o papel dos aspectos históricos, estéticos, midiáticos, transculturais, socioeconômicos, raciais e de gênero. Pesquisas atuais realizadas em diversas culturas demonstram a existência de uma relação entre esses fatores e os índices de anorexia nervosa e bulimia nervosa. A busca por um padrão estético globalizado (magreza, segundo a literatura, tem um papel central no aumento do número de casos. O objetivo deste artigo é apresentar uma discussão teórica acerca dos aspectos culturais envolvidos no aumento do número de casos de transtornos alimentares. Este trabalho pretende também destacar a necessidade de que profissionais de diversas áreas estejam atentos para os perigos do padrão de beleza atual, a fim de potencializar intervenções preventivas.Los transtornos alimentarios son patologías caracterizadas primordialmente por el miedo mórbido de engordar. Varios son los factores involucrados ya sea en la etiología cuanto en la manutención y en la gravedad de esas enfermidades: individuales, familiares y culturales. Con relación a esos últimos, se resalta el papel de los aspectos históricos, estéticos, midiáticos, transculturales, socio económicos, raciales y de género. Investigaciones actuales realizadas en diferentes culturas muestran la existencia de una relación entre esos factores y los índices de anorexia nervosa y bulimia nervosa. El objetivo de ese artículo es presentar una discusión a cerca de los aspectos culturales involucrados en el aumento del número de casos de transtornos alimentarios. Ese trabajo pretende también destacar la necesidad de que profesionistas de diversas áreas estean

  6. Transmission OF Campylobacter coli in chicken embryos

    Science.gov (United States)

    Rossi, Daise Aparecida; Fonseca, Belchiolina Beatriz; de Melo, Roberta Torres; Felipe, Gutembergue da Silva; da Silva, Paulo Lourenço; Mendonça, Eliane Pereira; Filgueiras, Ana Luzia Lauria; Beletti, Marcelo Emilio

    2012-01-01

    Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods; in the SPF eggs, however, the bacterium was detected by both techniques. The results showed no relationship between embryo mortality and positivity for C. coli in the embryos from breeder hens. However, the presence of bacteria is a cause of precocious mortality for SPF embryos. This study revealed that although the vertical transmission is a possible event, the bacteria can not grow in embryonic field samples. PMID:24031861

  7. Behavior of non-O157 Shiga toxin-producing Escherichia coli, enteroinvasive E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli strains on alfalfa sprouts.

    Science.gov (United States)

    Gómez-Aldapa, Carlos A; Rangel-Vargas, Esmeralda; Torres-Vitela, M Del Refugio; Villarruel-López, Angélica; Castro-Rosas, Javier

    2013-08-01

    Data about the behavior of non-O157 Shiga toxin-producing Escherichia coli (non-O157 STEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), and enteropathogenic E. coli (EPEC) on seeds and alfalfa sprouts are not available. The behavior of STEC, EIEC, ETEC, and EPEC was determined during germination and sprouting of alfalfa seeds at 20 ± 2°C and 30 ± 2°C and on alfalfa sprouts at 3 ± 2°C. When alfalfa seeds were inoculated with STEC, EIEC, ETEC, or EPEC strains, all these diarrheagenic E. coli pathotypes (DEPs) grew during germination and sprouting of seeds, reaching counts of approximately 5 and 6 log CFU/g after 1 day at 20 ± 2°C and 30 ± 2°C, respectively. However, when the sprouts were inoculated after 1 day of seed germination and stored at 20 ± 2°C or 30 ± 2°C, no growth was observed for any DEP during sprouting at 20 ± 2°C or 30 ± 2°C for 9 days. Refrigeration reduced significantly (P < 0.0.5) the number of viable DEPs on sprouts after 20 days in storage; nevertheless, these decreases have no practical significance for the safety of the sprouts.

  8. Transmission of Campylobacter coli in chicken embryos

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    Daise Aparecida Rossi

    2012-06-01

    Full Text Available Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods; in the SPF eggs, however, the bacterium was detected by both techniques. The results showed no relationship between embryo mortality and positivity for C. coli in the embryos from breeder hens. However, the presence of bacteria is a cause of precocious mortality for SPF embryos. This study revealed that although the vertical transmission is a possible event, the bacteria can not grow in embryonic field samples.

  9. Sensitivity Trend of E.coli

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    Molaabaszadeh H

    2013-03-01

    Full Text Available ABSTRACT Background and objective: The resistance to antibiotics among of pathogen bacteria is one of the main concerns of doctors all around the world, with consideration to different reports about E.coli bacteria’s sensitivity, this study was done to examine the pattern of sensitivity and antibiotic resistance of E.coli strains collected from clinical samples of patients hospitalized in Tehran’s Araad hospital. Materials and methods: In this descriptive examination, after extracting E.coli derivations from clinical samples (urine, catheter, phlegm, wound, abscess, vagina and blood, their sensitivity was measured using standard Kirby-Bauer test, in contract with following antibiotics Amikacin, Ciprofloxacin, Gentamicin, Imipenem, Sulfametoxazole Trimetoprime, Ceftriaxone and Cefotaxime. Results: Most of E. coli strains isolated were from urine samples every four years and the lowest of E. coli strains from vaginal samples. The most amount of sensibility to Imipenem and Amikacin and the most amount of resistant were seen to Sulfametoxazole-Trimetoprime and Ciprofloxacin. Conclusion: The results of this study are indicating that E.coli’s strains resistance has increased against Trimethoprim and Ciprofloxacin; presumably it is due to excessive consumption of these antibiotics. It is obvious that, with regard to increasing consumption of antibiotics, and consequently, augmentation of antibacterial resistance, control of this resistance factor is necessary and inevitable, so it is recommended to avoid unnecessary usage of antibiotics.

  10. REVIEW. BACTERIAS ACIDO LÁCTICAS: PAPEL FUNCIONAL EN LOS ALIMENTOS BACTÉRIAS ÁCIDO LÁCTICAS: PAPEL FUNCIONAL NOS ALIMENTOS REVIEW LACTIC ACID BACTERIA: FUNCTIONAL ROLE IN THE FOODS

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    RICARDO ADOLFO PARRA HUERTAS

    2010-06-01

    Full Text Available Las bacterias ácido lácticas han sido importantes en los alimentos por siglos por su considerable contribución al valor de los productos. Debido a varias de sus propiedades metabólicas, las bacterias ácido lácticas desempeñan un papel importante en la industria alimentaria, por su contribución significante al sabor, olor, textura, características sensoriales, propiedades terapéuticas y valor nutricional de los productos alimentarios. Este grupo está compuesto de un número de géneros incluyendo Lactococcus, Lactobacillus, Enterococcus, Streptococcus, Leuconostoc y Pediococcus. Algunos de los metabolitos producidos por las este tipo de bacterias son ácidos orgánicos, sustancias preservantes, polisacáridos, vitaminas, endulzantes, olores y sabores entre otros. Esta revisión se enfoca en estudiar la importancia de las bacterias acido lácticas en los alimentos.As bacterias ácido lácticas têm sido importantes nos alimentos por séculos pela sua considerável contribuição ao valor dos produtos. Devido a varias das suas propriedades metabóllcas, as bacterias ácido lácticas desempenham um papel importante na industria alimentar, pela sua contribulção significante ao sabor, cheiro, textura, características sensoriais, propriedades terapêuticas e valor nutrlcional dos produtos alimentar. Este grupo está constituido de um número de gêneros incluindo Lactococcus, Lactobaclllus, Enterococcus, Etreptococcus, Leuconostoc e Pediococcus. Alguns dos metabolltos produzidos por este tipo de bacterias são ácidos orgãnicos, sustãncias preservadoras, poli-sacáridos, vitaminas, adoçantes, cheiros e sabores entre outros. Esta revisão se enfoca em estudar a importãncia das bacterias ácido lácticas nos alimentos.Lactic acid bacteria have been important in food for centuries for their significant contribution to product value. Because several of its metabolic properties, lactic acid bacteria play an important role in the food

  11. El papel de los turoperadores en el desarrollo del turismo internacional. Una evaluación El papel de los turoperadores en el desarrollo del turismo internacional. Una evaluación

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    Nevenka Cavlek

    2005-09-01

    Full Text Available La intención de este artículo es analizar y explicar el papel de los turoperadores europeos en el desarrollo del turismo internacional desde 1950. Se analizará y se evaluará la influencia de los turoperadores en el desarrollo del turismo a través del uso de datos existentes que proceden de los principales mercados emisores y receptores de turismo. El desarrollo del turismo, en el que los turoperadores han participado de manera significativa, se caracteriza por una dinámica enormemente cambiante tanto en el área de la oferta de turismo como en el de la demanda. El análisis del papel de los turoperadores europeos en los flujos del turismo internacional muestra que la canalización de la demanda turística hacia destinos extranjeros se encuentra todavía muy influenciado por turoperadores de esos mercados que organizan el mayor número de viajes al extranjero. Como en la actualidad los más destacados turoperadores europeos se encuentran integrados verticalmente con compañías aéreas, agencias de viajes minoristas, instalaciones hoteleras y agencias de apoyo en los destinos, han adquirido el control tanto de los mercados emisores de turismo como de los receptores. Se presenta una evaluación de algunos aspectos positivos y negativos del desarrollo del turismo bajo la influencia de los turoperadores.The aim of this article is to analyze and explain the role played by European tour operators in international tourism development since 1950. By using existing data from the main tourism generating and receiving markets, the influence of tour operators on tourism development is analyzed and assessed. The phase of tourism development in which tour operators have played a significant part is characterized by highly dynamic changes both in the field of tourism supply as well as in tourism demand. The analysis of the role of European tour operators in international tourism flows shows that the movement of tourism demand from the most significant

  12. Extracción de fibras de agave para elaborar papel y artesanías Extracción de fibras de agave para elaborar papel y artesanías

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    Antonio Prieto Rodríguez

    2012-02-01

    Full Text Available Investigación aplicada para el aprovechamiento integral sustentable de vegetales nativos regionales, en la cual se extrajo fibra de seis especies de Agave (maguey; tres de hoja angosta (A. lechuguilla, A. angustifolia y A. tequilana y tres de hoja ancha (A. americana, A. salmiana y A. mapisaga, utilizando tres métodos; 1 inmersión en agua a cielo abierto, 2 fermentación con y sin “aguamiel”, y 3 cocción de pencas. Se evaluó: color, olor, suavidad, enguiche, facilidad de desfibración, y tiempo empleado. En conclusión, el tratamiento más práctico fue el cocimiento de pencas, aunque no el más económico y ecológico. Los resultados obtenidos hasta esta etapa indican que de cada especie analizada se puede obtener papel y que éste presenta características distintivas que lo hacen atractivo para diferentes usos artesanales. Particularmente, las especies que produjeron los tipos de papel de mayor calidad para la escritura y el dibujo fueron A. salmiana y A. mapisaga.   Applied research for the sustainable integral use of regional native plants, which was extracted fiber from six species of Agave (common name maguey, three narrow-leaved (A. lechuguilla, A. angustifolia and A. tequilana and three wide-leaved (A. americana, A. salmiana and A. mapisaga by using three methods: 1 cooking of pads, 2 fermentation with and without aguamiel (maguey sweet juice and 3 immersion in water open sky. By considering the variables color, odor, softness, enguiche, easiness of defibration and time spent. In conclusion, most practical treatment was the boiling of the fleshy leaves, but not the most economical and environmentally friendly one. The results got up to this stage indicate that paper can be obtained from each of the six species and that it shows characteristic features which make it attractive to be applied in various craft uses. In particular, the species pro­ducing the most paper with the best quality, i.e., for writing and drawing, were A

  13. O papel do enfermeiro como educador junto a cuidadores familiares de pessoas com AVC El papel del enfermero como educador de cuidadores familiares de personas con AVC The nurse's role as an educator toward people who care for BVA patients

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    Silvia Cristina Mangini Bocchi

    2004-10-01

    Full Text Available Trata-se de uma pesquisa do tipo bibliográfico com a finalidade de fazer uma análise temática da produção do conhecimento em periódicos, sobre o papel do enfermeiro como educador junto a cuidadores familiares de pessoas com AVC (Acidente Vascular Cerebral. O corpus de análise reuniu artigos localizados nas décadas de 80 e 90, a partir das bases de dados Medline, Lilacs e Cinahl. A análise de conteúdo foi o referencial metodológico que permitiu organizar o conhecimento em duas categorias: a intervenção educacional na fase aguda e o suporte após a alta hospitalar.Este trabajo es una investigación bibliográfica que tiene la finalidad de hacer un análisis temático de producción del conocimiento en periódicos, sobre el papel del enfermero educador de cuidadores familiares de personas con AVC (Accidente Vascular Cerebral. El corpus de análisis reunió artículos realizados en las décadas de 80 y 90 a partir de las bases de datos Mediline, Lilacs y Cinahl. El análisis del contenido fue el referencial teórico que permitió organizar el conocimiento en dos categorías: la intervención educativa en la fase aguda y el soporte después del alta hospitalario.This article was written based on bibliographical research aiming at making a thematic analysis of the production of knowledge in newspapers and magazines about the nurse's role as an educator toward people who care for BVA (brain vascular accident patients. The analysis gathered articles published in the 80's and 90's, from the Medline, Lilacs and Cinahl databases. Content analysis was the methodological background that allowed the organization of knowledge in two categories: educational intervention in the acute stage and the post-discharge support.

  14. Optical properties of the Eu(III)-La(III)-complex-doped polyolefine film and rod samples

    Science.gov (United States)

    Pogreb, Roman; Popov, Oleg; Lirtsman, Vlad; Pyshkin, Oleg; Kazachkov, Alexander; Musin, Albina; Finkelshtein, Binyamin; Shmukler, Yuri; Davidov, Dan; Bormashenko, Edward

    2005-04-01

    The work is devoted to luminescent properties of trivalent lanthanide complexes dispersed in thermoplastic host matrices. Polyethylene-based film and polypropylene-based rod both doped with these complexes were manufactured using an extrusion technique. Two kinds of dopants were used: Eu(III)-thenoyltrifluoroacetone-1,10-phenanthroline complex (Eu(III)) and Eu(III)-La(III)-1,10-phenanthroline complex (Eu(III)-La(III)). Comparison was made between these samples regarding absorption, excitation, emission and a lifetime of luminescence. Dependence of emission intensity on the excitation energy was determined. Emission spectra of the films were studied at room and helium temperatures. Optical properties of Eu(III) samples are different from Eu(III)-La(III) samples. Significant difference in spectra of these two types of samples may be attributed to the La(III) action.

  15. Development of demographic norms for four new WAIS-III/WMS-III indexes.

    Science.gov (United States)

    Lange, Rael T; Chelune, Gordon J; Taylor, Michael J; Woodward, Todd S; Heaton, Robert K

    2006-06-01

    Following the publication of the third edition Wechsler scales (i.e., WAIS-III and WMS-III), demographically corrected norms were made available in the form of a computerized scoring program (i.e., WAIS-III/WMS-III/WIAT-II Scoring Assistant). These norms correct for age, gender, ethnicity, and education. Since then, four new indexes have been developed: the WAIS-III General Ability Index, the WMS-III Delayed Memory Index, and the two alternate Immediate and Delayed Memory Indexes. The purpose of this study was to develop demographically corrected norms for the four new indexes using the standardization sample and education oversample from the WAIS-III and WMS-III. These norms were developed using the same methodology as the demographically corrected norms made available in the WAIS-III/WMS-III/WIAT-II Scoring Assistant.

  16. Remoção de cálcio de efluente de máquina de papel por precipitação/coprecipitação

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    José Carlos Leandro de Sousa

    2011-01-01

    Full Text Available Nas fábricas integradas de celulose e papel, o efluente gerado pela máquina de papel pode ser considerado um efluente setorial,denominado de água branca, devido à concentração elevada de cálcio. Neste trabalho foram realizados experimentos com objetivos decompreender o comportamento do efluente em função do pH e desenvolver metodologias de remoção de cálcio da água branca, demodo a proporcionar o reuso da água e a recuperação de cálcio. Titulações potenciométricas foram realizadas com HCl 0,022 mol L-1 ecom NaOH 0,025 mol L-1padronizados, após ajuste de pH do efluente em 12,0 e 2,0 respectivamente, às quais indicaram pontos deinflexão referentes à carbonato, bicarbonato e caulim, componentes com capacidade de interação com o cálcio solúvel. Os métodos deremoção de cálcio consistiram de coprecipitação/adsorção com hidróxidos de ferro (III e de alumínio e precipitação na presença deoxalato de sódio. Os resultados apontaram que em baixas concentrações de sulfato férrico e sulfato de alumínio, a coprecipitação decálcio é baixa. Nos ensaios de adsorção na presença de Fe(OH3 e Al(OH3, notou-se que o aumento da concentração de sulfato férricopossibilitou um ligeiro aumento da remoção de cálcio (16,5 a 31,0%, chegando a 65,0% na soma dos processos deadsorção/precipitação em pH 10,0. No caso do sulfato de alumínio as porcentagens de remoção foram indiferentes (~ 10,0%. Emrelação à precipitação de Ca2+ na presença de oxalato, observou-se remoção de porcentagens satisfatórias na forma de oxalato decálcio (75% a 87%, mantendo-se o efluente com condutividade e pH praticamente inalterados, o que é muito desejável, já que oaumento da condutividade do efluente tratado na reutilização provoca efeito de quebra das folhas de papel. O oxalato de cálciorecuperado pode ser calcinado, sendo convertido a carbonato de cálcio, para posterior reuso. Testes em escala piloto devem

  17. Cellular chain formation in Escherichia coli biofilms

    DEFF Research Database (Denmark)

    Vejborg, Rebecca Munk; Klemm, Per

    2009-01-01

    In this study we report on a novel structural phenotype in Escherichia coli biofilms: cellular chain formation. Biofilm chaining in E. coli K-12 was found to occur primarily by clonal expansion, but was not due to filamentous growth. Rather, chain formation was the result of intercellular......; type I fimbriae expression significantly reduced cellular chain formation, presumably by steric hindrance. Cellular chain formation did not appear to be specific to E coli K-12. Although many urinary tract infection (UTI) isolates were found to form rather homogeneous, flat biofilms, three isolates......, including the prototypic asymptomatic bacteriuria strain, 83972, formed highly elaborate cellular chains during biofilm growth in human urine. Combined, these results illustrate the diversity of biofilm architectures that can be observed even within a single microbial species....

  18. Interaction between Escherichia coli and lunar fines

    Science.gov (United States)

    Johansson, K. R.

    1983-01-01

    A sample of mature lunar fines (10084.151) was solubilized to a high degree (about 17 percent) by the chelating agent salicylic acid (0.01. M). The neutralized (pH adjusted to 7.0) leachate was found to inhibit the growth of Escherichia coli (ATCC 259922) in a minimial mineral salts glucose medium; however, the inhibition was somewhat less than that caused by neutralized salicylic acid alone. The presence of lunar fines in the minimal medium was highly stimulatory to growth of E. coli following an early inhibitory response. The bacterium survived less well in the lunar leachate than in distilled water, no doubt because of the salicylate. It was concluded that the sample of lunar soil tested has nutritional value to E. coli and that certain products of fermentation helped to solubilize the lunar soil.

  19. A Multi-Country Cross-Sectional Study of Vaginal Carriage of Group B Streptococci (GBS and Escherichia coli in Resource-Poor Settings: Prevalences and Risk Factors.

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    Piet Cools

    Full Text Available One million neonates die each year in low- and middle-income countries because of neonatal sepsis; group B Streptococcus (GBS and Escherichia coli are the leading causes. In sub-Saharan Africa, epidemiological data on vaginal GBS and E. coli carriage, a prerequisite for GBS and E. coli neonatal sepsis, respectively, are scarce but necessary to design and implement prevention strategies. Therefore, we assessed vaginal GBS and E. coli carriage rates and risk factors and the GBS serotype distribution in three sub-Saharan countries.A total of 430 women from Kenya, Rwanda and South Africa were studied cross-sectionally. Vaginal carriage of GBS and E. coli, and GBS serotype were assessed using molecular techniques. Risk factors for carriage were identified using multivariable logistic regression analysis.Vaginal carriage rates in reference groups from Kenya and South Africa were 20.2% (95% CI, 13.7-28.7% and 23.1% (95% CI, 16.2-31.9%, respectively for GBS; and 25.0% (95% CI, 17.8-33.9% and 27.1% (95% CI, 19.6-36.2%, respectively for E. coli. GBS serotypes Ia (36.8%, V (26.3% and III (14.0% were most prevalent. Factors independently associated with GBS and E. coli carriage were Candida albicans, an intermediate vaginal microbiome, bacterial vaginosis, recent vaginal intercourse, vaginal washing, cervical ectopy and working as a sex worker. GBS and E. coli carriage were positively associated.Reduced vaginal GBS carriage rates might be accomplished by advocating behavioral changes such as abstinence from sexual intercourse and by avoidance of vaginal washing during late pregnancy. It might be advisable to explore the inclusion of vaginal carriage of C. albicans, GBS, E. coli and of the presence of cervical ectopy in a risk- and/or screening-based administration of antibiotic prophylaxis. Current phase II GBS vaccines (a trivalent vaccine targeting serotypes Ia, Ib, and III, and a conjugate vaccine targeting serotype III would not protect the majority of

  20. Intramammary challenge with Escherichia coli following immunization with a curli-producing Escherichia coli.

    Science.gov (United States)

    Todhunter, D A; Smith, K L; Hogan, J S; Nelson, L

    1991-03-01

    Holstein and Jersey cattle were immunized with a curli-producing strain of Escherichia coli (pCRL65/A012) or a noncurli-producing strain (pUC18/HB101) to determine differences in resistance to establishment of experimental intramammary infection. Cows (n = 6 per group) were immunized at 14 d prior to drying off, 7 d of involution, and at calving with 3 x 10(10) E. coli in Freund's Incomplete Adjuvant. At 30 d of lactation, one mammary quarter of each cow was infused with a wild strain of E. coli (727). Escherichia coli 727 was isolated from a naturally occurring intramammary infection and produced curli. All challenged quarters became infected, and all cows developed acute clinical mastitis. Geometric mean duration of intramammary infections was 6 d for both immunization groups. All infections were spontaneously eliminated within 10 d. No differences occurred between immunization groups in blood selenium and glutathione peroxidase activity, plasma selenium, number of E. coli 727 isolated from secretion after challenge, rectal temperature and SCC response, clinical status of mammary quarters, or DMI. Reduction in milk production after challenge was greater for cows immunized with E. coli pCRL65/A012. Immunization of dairy cattle with a curli-producing strain of E. coli did not protect against experimental intramammary challenge during lactation.