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  1. Economic and environmental impact of the utilization of the industrial potential of viable cogeneration for period 1998 - 2007; Impacto economico y ambiental de aprovechamiento del potencial industrial de cogeneracion viable para el periodo 1998 - 2007

    Energy Technology Data Exchange (ETDEWEB)

    Leon de los Santos, Gabriel; Mendoza Gonzslez, Lourdes [Universidad Nacional Autonoma de Mexico, Mexico, D.F. (Mexico)

    2001-07-01

    The utilization of the industrial potential of cogeneration offers a significant contribution to the construction of the electrical infrastructure that the growth of the country will require for the period 1998 - 2007. The conditions of growth of the co-generation potential and their levels of utilization are related to a on isolated growth of the other inter actors of the processes of power supply; As levels of economic viability, economic savings to industry, requirements of additional electrical capacity, growth of the industrial activity, costs, use of fuels, environmental impact. Rates of average growth for period 1998 - 2007 are considered and what levels of economic and environmental benefit offers this development to the industry, to the electrical company and to the country. And to what proportion can contribute the utilization of the industrial potential of electrical cogeneration to the requirements of additional electrical capacity that will require the development of the country during this period. With the rates of viable growth of the co-generation the equivalent reduction of fuel consumption is estimated for the industrial sector, given by the improvement in the generation efficiency and with the change in the proportion of fuels used in Comision Federal de Electricidad (CFE) for this period. Evaluating the emissions of the main fuels avoided with this growth and its repercussion at national level via the CFE, and showing the possible international implications of this reduction. [Spanish] El aprovechamiento del potencial industrial de cogeneracion ofrece una significativa aportacion a la construccion de la infraestructura electrica que el crecimiento del pais requerira para el periodo 1998 -2007. Las condiciones de crecimiento del potencial de cogeneracion y sus niveles de aprovechamiento estan relacionados con un crecimiento o aislado de los demas inter actores de los procesos de abasto energetico; Como niveles de viabilidad economica, ahorros

  2. Cogeneration Systems; Sistemas de Cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez M, Manuel F; Huante P, Liborio; Romo M, Cesar A [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2006-07-01

    The present article deals on relevant aspects on the subject of cogeneration within the Mexican territorial limits. In the first place it is presented the role of Mexico in terms of its cogeneration potential, the type of service that has obtained from this predominant modality of cogeneration for self-supplying, the most propitious sectors to develop it, its legislations on the matter, the projects made for the implementation of cogeneration plants, as well as the existing cogeneration schemes for its respective optimization proposals. Without leaving out the analysis on the different types of evaluation on the efficiency of cogeneration systems and the aspects to consider for the election of a generation cycle. [Spanish] El presente articulo trata sobre aspectos relevantes en materia de cogeneracion dentro de los limites territoriales de la nacion mexicana. Se muestra en primer lugar el papel de Mexico en terminos de su potencial de cogeneracion, el tipo de servicio que ha obtenido de esta predominantemente (modalidad de cogeneracion para autoabastecimiento), los sectores mas propicios para desarrollarla, sus legislaciones al respecto, los proyectos realizados para la implementacion de plantas de cogeneracion, asi como los esquemas de cogeneracion existentes con sus respectivas propuestas de optimizacion. Sin dejar de lado el analisis sobre los distintos tipos de evaluacion de la eficiencia de sistemas de cogeneracion y los aspectos a considerar para la eleccion de un ciclo de generacion.

  3. Feasibility of the operation CFE`s conventional power plants for industrial cogeneration; Factibilidad de operar las centrales termoelectricas convencionales de la CFE para cogeneracion industrial

    Energy Technology Data Exchange (ETDEWEB)

    Buendia Dominguez, Eduardo H.; Acosta Torres, Rosa Aracely [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1997-12-31

    An analysis is made on how cogeneration could help to maximize the power plant performance since thanks to cogeneration this type of power plants could utilize a part of the waste energy to be supplied to other processes. Also mention is made of the utilization of computer programs to help the design and the realization of functioning tests of this type of power plants. An economic evaluation is presented on the feasibility of operating this type of power plants as cogeneration units and it is concluded that the operation of fossil power plants in the cogeneration mode is not affected by the site where the power plant is located [Espanol] Se analiza como la cogeneracion podria ayudar a maximizar el rendimiento de las centrales termoelectricas ya que gracias a la cogeneracion este tipo de centrales podrian utilizar una parte de la energia desechada para suministrarla a otros procesos. Tambien se menciona la utilizacion de programas de computo para auxiliar en el diseno o realizacion de pruebas de funcionamiento sobre este tipo de plantas. Se presenta una evaluacion economica sobre la factibilidad de operar este tipo de plantas como unidades de cogeneracion y se concluye en que la operacion de centrales termoelectricas en modo de cogeneracion no se ve afectado por el sitio donde se encuentra la central

  4. Feasibility of the operation CFE`s conventional power plants for industrial cogeneration; Factibilidad de operar las centrales termoelectricas convencionales de la CFE para cogeneracion industrial

    Energy Technology Data Exchange (ETDEWEB)

    Buendia Dominguez, Eduardo H; Acosta Torres, Rosa Aracely [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1998-12-31

    An analysis is made on how cogeneration could help to maximize the power plant performance since thanks to cogeneration this type of power plants could utilize a part of the waste energy to be supplied to other processes. Also mention is made of the utilization of computer programs to help the design and the realization of functioning tests of this type of power plants. An economic evaluation is presented on the feasibility of operating this type of power plants as cogeneration units and it is concluded that the operation of fossil power plants in the cogeneration mode is not affected by the site where the power plant is located [Espanol] Se analiza como la cogeneracion podria ayudar a maximizar el rendimiento de las centrales termoelectricas ya que gracias a la cogeneracion este tipo de centrales podrian utilizar una parte de la energia desechada para suministrarla a otros procesos. Tambien se menciona la utilizacion de programas de computo para auxiliar en el diseno o realizacion de pruebas de funcionamiento sobre este tipo de plantas. Se presenta una evaluacion economica sobre la factibilidad de operar este tipo de plantas como unidades de cogeneracion y se concluye en que la operacion de centrales termoelectricas en modo de cogeneracion no se ve afectado por el sitio donde se encuentra la central

  5. Co-generation and reality Potential in Mexico; Potencial de cogeneracion y realidad en Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Comision Nacional para el Ahorro de Energia (CONAE) (Mexico)

    2005-07-01

    This document deals with the Mexican use of co-generation -the efficient use of the energy- through the support offered by the Comision Nacional para el Ahorro de Energia (CONAE), since this is the agency in charge of fomenting the efficient use of energy by means of actions coordinated with diverse dependencies and organizations of the Administracion Publica Federal and with the governments of the federal entities as well as municipalities, social and private sectors. Among the subjects to be dealt are quality of the electrical and thermal energy, types of fuels that can be used in the co-generation project, the present situation of the co-generation in Mexico and the conditions for their development. [Spanish] Este documento analiza el uso de la cogeneracion en Mexico es decir, el uso eficiente de la energia a traves del apoyo que brinda la Comision Nacional para el Ahorro de Energia (CONAE) ya que es el organo encargado de fomentar la eficiencia en el uso de la energia mediante acciones coordinadas con las diversas dependencias y entidades de la Administracion Publica Federal y con los gobiernos de las entidades federativas y los municipios y, a traves de acciones concertadas, con los sectores social y privado. Se trataran temas como calidad de la energia electrica y termica, los tipos de combustibles que pueden utilizarse en el proyecto de cogeneracion, la situacion actual de la cogeneracion en Mexico y las ccondiciones para su desarrollo.

  6. Application of the Pinch analysis for the design of a cogeneration system in a paper mill; Aplicacion del analisis Pinch para el diseno de un sistema de cogeneracion en una industria papelera

    Energy Technology Data Exchange (ETDEWEB)

    Mani Gonzalez, A. G.; Arriola Medellin, A. [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1997-12-31

    The Pinch Analysis is a set of principles, tools and rules for the design that allow the engineer find the best way to configure the elements of a process. In the last ten years it has been utilized for the design of new processes as well as in the energy optimization of existing processes. In this paper the tools utilized for the integration of a cogeneration system in a process for the production of paper is presented. It is also presented how the combined treatment of the Pinch Analysis and the exergy concept allows to define, before the detailed design, the cogeneration potential, the fuel consumption and the amount of pollutant emissions for different cogeneration schemes. [Espanol] El analisis Pinch es un conjunto de principios, herramientas y reglas de diseno que permiten al ingeniero encontrar la mejor manera de configurar los elementos de un proceso. En los ultimos diez anos se ha utilizado para el diseno de procesos nuevos asi como en la optimacion energetica de procesos existentes. En el presente articulo se presentan las herramientas utilizadas para la integracion de un sistema de cogeneracion en un proceso de produccion de papel. Se muestra tambien como el tratamiento combinado del analisis Pinch y el concepto de energia permite definir, antes del diseno detallado, el potencial de cogeneracion, el consumo de combustible y la cantidad de emisiones contaminantes para diferentes esquemas de cogeneracion.

  7. Application of the Pinch analysis for the design of a cogeneration system in a paper mill; Aplicacion del analisis Pinch para el diseno de un sistema de cogeneracion en una industria papelera

    Energy Technology Data Exchange (ETDEWEB)

    Mani Gonzalez, A G; Arriola Medellin, A [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1998-12-31

    The Pinch Analysis is a set of principles, tools and rules for the design that allow the engineer find the best way to configure the elements of a process. In the last ten years it has been utilized for the design of new processes as well as in the energy optimization of existing processes. In this paper the tools utilized for the integration of a cogeneration system in a process for the production of paper is presented. It is also presented how the combined treatment of the Pinch Analysis and the exergy concept allows to define, before the detailed design, the cogeneration potential, the fuel consumption and the amount of pollutant emissions for different cogeneration schemes. [Espanol] El analisis Pinch es un conjunto de principios, herramientas y reglas de diseno que permiten al ingeniero encontrar la mejor manera de configurar los elementos de un proceso. En los ultimos diez anos se ha utilizado para el diseno de procesos nuevos asi como en la optimacion energetica de procesos existentes. En el presente articulo se presentan las herramientas utilizadas para la integracion de un sistema de cogeneracion en un proceso de produccion de papel. Se muestra tambien como el tratamiento combinado del analisis Pinch y el concepto de energia permite definir, antes del diseno detallado, el potencial de cogeneracion, el consumo de combustible y la cantidad de emisiones contaminantes para diferentes esquemas de cogeneracion.

  8. Boehringer Ingelheim Promeco contemplates the benefits of cogeneration in its new plant; Boehringer Ingelheim Promeco contempla beneficios de la cogeneracion para su nueva planta

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz Esparza, R. [Instituto de Investigaciones Electricas, Cuernavaca (Mexico); Khouri Solis, A. [Boehringer Ingelheim Promeco, Mexico, D. F. (Mexico)

    1997-12-31

    This paper presents the results of the feasibility study performed in BOEHRINGER INGELHEIM PROMECO to determine the benefits that a cogeneration system could render in satisfying the energy demand of its plant currently being expanded. The results showed that in accordance with the operation mode of the already expanded plant, the highest benefits could be obtained of a cogeneration system with a capacity of 1,600 Kw that would partially satisfy the thermal and electrical demands of BOEHRINGER INGELHEIM PROMECO. [Espanol] El presente articulo presenta los resultados de factibilidad que se realizo en Boehringer Ingelheim Promeco para determinar los beneficios que podria rendir un sistema de cogeneracion al satisfacer las demandas energeticas de su planta actualmente en ampliacion. Los resultados mostraron que de acuerdo al modo de operacion de la planta ya ampliada, los beneficios mas altos se obtendrian de un sistema de cogeneracion con capacidad de 1,600 kW que satisfaria parcialmente las demandas termicas y electricas de Boehringer Ingelheim Promeco.

  9. Boehringer Ingelheim Promeco contemplates the benefits of cogeneration in its new plant; Boehringer Ingelheim Promeco contempla beneficios de la cogeneracion para su nueva planta

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz Esparza, R [Instituto de Investigaciones Electricas, Cuernavaca (Mexico); Khouri Solis, A [Boehringer Ingelheim Promeco, Mexico, D. F. (Mexico)

    1998-12-31

    This paper presents the results of the feasibility study performed in BOEHRINGER INGELHEIM PROMECO to determine the benefits that a cogeneration system could render in satisfying the energy demand of its plant currently being expanded. The results showed that in accordance with the operation mode of the already expanded plant, the highest benefits could be obtained of a cogeneration system with a capacity of 1,600 Kw that would partially satisfy the thermal and electrical demands of BOEHRINGER INGELHEIM PROMECO. [Espanol] El presente articulo presenta los resultados de factibilidad que se realizo en Boehringer Ingelheim Promeco para determinar los beneficios que podria rendir un sistema de cogeneracion al satisfacer las demandas energeticas de su planta actualmente en ampliacion. Los resultados mostraron que de acuerdo al modo de operacion de la planta ya ampliada, los beneficios mas altos se obtendrian de un sistema de cogeneracion con capacidad de 1,600 kW que satisfaria parcialmente las demandas termicas y electricas de Boehringer Ingelheim Promeco.

  10. Cogeneration in large processing power stations; Cogeneracion en grandes centrales de proceso

    Energy Technology Data Exchange (ETDEWEB)

    Munoz, Jose Manuel [Observatorio Ciudadano de la Energia A. C., (Mexico)

    2004-06-15

    , cervecerias, papeleras y plantas de celulosa, entre otras, con necesidades de vapor para sus procesos, tienen las condiciones tecnicas y economicas para cogenerar o sea, producir simultaneamente vapor y electricidad. De hecho, muchas de tales instalaciones que existen actualmente en cualquier pais, cuentan con equipos de cogeneracion que les permiten obtener su electricidad a un costo muy bajo, aprovechando la existencia de generadores de vapor que de cualquier forma son indispensables para satisfacer su demanda. En Mexico, dado el marco legal existente, tanto el servicio publico de electricidad como la industria petrolera son actividades de caracter obligatorio para el estado por estas razones, el tema debiera ser parte de la agenda de planeacion de dicho sector energetico. Las oportunidades a las que nos referimos, son validas para las industrias pequenas, pero desde el punto de vista del interes nacional, son mas importantes para las instalaciones de gran tamano y en ese rango, las mas numerosas estan precisamente en PEMEX, mientras que los grandes excedentes de energia y capacidad que resultarian de cogeneraciones en refinerias y petroquimicas serian de un alto valor, precisamente para el servicio publico de electricidad, o sea para la Comision Federal de Electricidad (CFE).

  11. Cogeneration in a plywood factory; Sistema de cogeneracion en una planta maderera

    Energy Technology Data Exchange (ETDEWEB)

    Castelazo Hernandez, Arturo; Mendoza Arcaraz, Alfonso [Ultra Energia, S. A. de C. V. Naucalpan (Mexico)

    1996-12-31

    back-pressure steam turbine. The back-pressure steam turbine exhaust will be used to heat up the thermal oil through a steam-thermal oil heat exchanger. [Espanol] La Cogeneracion, que es la produccion conjunta de dos manifestaciones de energia a partir de una misma fuente energetica, se presenta como una de las alternativas mas viables para lograr el aprovechamiento optimo de los recursos energeticos finitos. En las ultimas decadas en que el crecimiento economico de Mexico ha estado ligado a la generacion y suministro de energia electrica, el Estado ha fungido como organizador y director del sistema nacional de generacion electrica, sin embargo la intervencion de los particulares ha sido determinante. Debemos tomar en cuenta que para cubrir las necesidades futuras de electricidad del desarrollo nacional, se requeriran aproximadamente 17 mil MW adicionales, para lo cual la iniciativa privada, entre las que podemos destacar a la pequena y mediana industria, tendra despejado el camino para producirlos, aprovechando los cambios en la Ley y el Reglamento del Servicio Publico de Energia Electrica, publicados en el Diario Oficial de la Federacion los dias 23 de diciembre de 1992 y 31 de mayo de 1993 respectivamente. Al contar con un sistema de cogeneracion optimizado, el importe economico anual de la empresa maderera por concepto de energia (electricidad y combustible) se reducira sustancialmente. La empresa maderera requiere la instalacion de un sistema de cogeneracion que suministre 4.75 MWe y 1,861 KWt (1.6 Gcal/hr). La energia electrica se utilizara en la fabricacion de TABLONES DE NOVOPAN y la energia termica para calentar aceite que se utiliza en la presa de madera y en la preparacion de la emulsion utilizada en el proceso. Para satisfacer los requerimientos energeticos se han analizado 2 alternativas: 1. Generacion de energia electrica con un motor de combustion interna que utilice combustoleo como combustible, aprovechandose los gases de escape del motor para pre

  12. Cogeneration in a plywood factory; Sistema de cogeneracion en una planta maderera

    Energy Technology Data Exchange (ETDEWEB)

    Castelazo Hernandez, Arturo; Mendoza Arcaraz, Alfonso [Ultra Energia, S. A. de C. V. Naucalpan (Mexico)

    1997-12-31

    back-pressure steam turbine. The back-pressure steam turbine exhaust will be used to heat up the thermal oil through a steam-thermal oil heat exchanger. [Espanol] La Cogeneracion, que es la produccion conjunta de dos manifestaciones de energia a partir de una misma fuente energetica, se presenta como una de las alternativas mas viables para lograr el aprovechamiento optimo de los recursos energeticos finitos. En las ultimas decadas en que el crecimiento economico de Mexico ha estado ligado a la generacion y suministro de energia electrica, el Estado ha fungido como organizador y director del sistema nacional de generacion electrica, sin embargo la intervencion de los particulares ha sido determinante. Debemos tomar en cuenta que para cubrir las necesidades futuras de electricidad del desarrollo nacional, se requeriran aproximadamente 17 mil MW adicionales, para lo cual la iniciativa privada, entre las que podemos destacar a la pequena y mediana industria, tendra despejado el camino para producirlos, aprovechando los cambios en la Ley y el Reglamento del Servicio Publico de Energia Electrica, publicados en el Diario Oficial de la Federacion los dias 23 de diciembre de 1992 y 31 de mayo de 1993 respectivamente. Al contar con un sistema de cogeneracion optimizado, el importe economico anual de la empresa maderera por concepto de energia (electricidad y combustible) se reducira sustancialmente. La empresa maderera requiere la instalacion de un sistema de cogeneracion que suministre 4.75 MWe y 1,861 KWt (1.6 Gcal/hr). La energia electrica se utilizara en la fabricacion de TABLONES DE NOVOPAN y la energia termica para calentar aceite que se utiliza en la presa de madera y en la preparacion de la emulsion utilizada en el proceso. Para satisfacer los requerimientos energeticos se han analizado 2 alternativas: 1. Generacion de energia electrica con un motor de combustion interna que utilice combustoleo como combustible, aprovechandose los gases de escape del motor para pre

  13. Prefeasibility analysis of a cogeneration project in the Mexican food industry; Analisis de prefactibilidad de un proyecto de cogeneracion en la industria alimenticia mexicana

    Energy Technology Data Exchange (ETDEWEB)

    Gongora Gonzalez, Guillermo; Castelazo Hernandez, Arturo [Ultra Energia, S. A. de C. V. Naucalpan (Mexico)

    1994-12-31

    The main technical and economical results of a prefeasibility study of an industry considered of medium size in the food area, which is a subsidiary of an American industrial merger, are shown. The above mentioned facility is located in the highland zone, its installed capacity is 2,350 electrical kw and 8,519 thermal kw needed for the optimum development of the productive process. Five technically feasible cogeneration options are analyzed, comparing the advantages and disadvantages of each option. Guides and conclusions for this type of projects are presented . [Espanol] Se muestran los principales resultados tecnicos y economicos de un estudio de prefactibilidad de una industria considerada como mediana de la rama alimenticia, la cual es filial de un consorcio industrial norteamericano. La planta mencionada se encuentra localizada en la zona del altiplano, cuya capacidad instalada es de 2,350 kW electricos y 8,519 kW termicos necesarios para el desarrollo optimo del proceso productivo. Se analizan cinco alternativas de cogeneracion tecnicamente viables, comparando las ventajas y desventajas de cada alternativa. Se presentan las recomendaciones y conclusiones para este tipo de proyectos.

  14. Prefeasibility analysis of a cogeneration project in the Mexican food industry; Analisis de prefactibilidad de un proyecto de cogeneracion en la industria alimenticia mexicana

    Energy Technology Data Exchange (ETDEWEB)

    Gongora Gonzalez, Guillermo; Castelazo Hernandez, Arturo [Ultra Energia, S. A. de C. V. Naucalpan (Mexico)

    1993-12-31

    The main technical and economical results of a prefeasibility study of an industry considered of medium size in the food area, which is a subsidiary of an American industrial merger, are shown. The above mentioned facility is located in the highland zone, its installed capacity is 2,350 electrical kw and 8,519 thermal kw needed for the optimum development of the productive process. Five technically feasible cogeneration options are analyzed, comparing the advantages and disadvantages of each option. Guides and conclusions for this type of projects are presented . [Espanol] Se muestran los principales resultados tecnicos y economicos de un estudio de prefactibilidad de una industria considerada como mediana de la rama alimenticia, la cual es filial de un consorcio industrial norteamericano. La planta mencionada se encuentra localizada en la zona del altiplano, cuya capacidad instalada es de 2,350 kW electricos y 8,519 kW termicos necesarios para el desarrollo optimo del proceso productivo. Se analizan cinco alternativas de cogeneracion tecnicamente viables, comparando las ventajas y desventajas de cada alternativa. Se presentan las recomendaciones y conclusiones para este tipo de proyectos.

  15. Economic and environmental advantages for the utilization of the industrial potential of cogeneration in Mexico; Ventajas economicas y ambientales para el aprovechamiento del potencial industrial de cogeneracion en Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Leon de los Santos, G [Division de Estudios Posgrado, Facultad de ingenieria, Universidad Nacional Autonoma de Mexico, Mexico, D.F. (Mexico)

    2003-01-15

    Mexico has an industrial cogeneration potential very important, not evaluated or projected within its historical growth. The objective of the present work consist of studying the industrial cogeneration potential that exists in Mexico, as well as the economic and environmental savings that it would be achieved if one took advantage of this potential. As a result of the present work we can conclude that the cogeneration in Mexico offers a theoretical potential middle of 28,000 MWe to the 2007. It is estimated an economic saving potential in the construction of generation infrastructure electrical, for the high stage of its utilization of almost 7000 million dollars and a stage of emissions reduction of 2007- 21.4%, instead of 2007- 0.35%, for the industrial sector of the country. Provided that change the vision of the government of seeing to the cogeneration as a measure of energy saving and substitute it by that of political of complement to the development of the national electrical sector. [Spanish] Mexico tiene un importante potencial de cogeneracion industrial que no ha sido evaluado ni proyectado dentro de su crecimiento historico, por ello, el objetito de este trabajo consiste en estudiar y conocer los ahorros economicos y ambientales que se podrian lograr si se aprovechara dicho potencial. Como resultado, se muestra que la cogeneracion en Mexico ofrece un potencial teorico medio de 28,000 MW e al ano 2007. Se estima tambien un ahorro economico en la construccion de infraestructura de generacion electrica para el escenario alto de su aprovechamiento de casi 7000 mdd, y un escenario de reduccion de emisiones para el sector industrial del pais de un 0.35% a un 21.47% para el ano 2007. Estos pronosticos se pueden lograr, siempre y cunado el gobierno cambie la vision de manejar este tema como una medida de ahorro de energia, y la sustituya por la politica de complemento al desarrollo del sector electrico nacional.

  16. Cogeneration and Carbon bonds: clean development; Cogeneracion y bonos de carbono: desarrollo limpio

    Energy Technology Data Exchange (ETDEWEB)

    Navarro Perez, Nidia [Facultad de Contaduria y Administracion, Universidad Nacional Autonoma de Mexico, Mexico, D.F. (Mexico)

    2004-06-15

    The growing preoccupation for the environment in our country and its interest to ratify the Kyoto Protocol with respect to the contamination of the atmosphere, offers great opportunities for the cogeneration so that it fortifies the scientific and technological research and gives a good international image about the sustainable development and care of the environment, so that companies that invest in clean technology will be able to assign a monetary value to their environmental patrimony, this through the so called Green Bonds or Carbon Bonds, this opens a new dimension to finance projects by means of these bonds that can be negotiated at an international level; by means of the Clean of Energy Production the investment can be stimulated and revenues for projects that contribute to the sustainable development of the country and the power efficiency. At the moment the country has at least 13 projects in different analysis stages to enter the carbon bond market, which are presented as co-generation projects of energy, in addition to the formation of the Mexican Committee for Projects of Reduction and Capture of Gas Discharges of Greenhouse Effect. [Spanish] La creciente preocupacion por el medio ambiente en nuestro pais y su interes por ratificar el Protocolo de Kyoto en lo referente a la contaminacion de la atmosfera, ofrece grandes oportunidades para la cogeneracion de manera que fortalezca la investigacion cientifica y tecnologica y dar una buena imagen internacional en torno a temas de desarrollo sustentable y cuidado del medio ambiente, de manera que empresas que invierten en tecnologia limpia podran asignar un valor monetario a su patrimonio ambiental, esto a traves de los llamados Bonos Verdes o Bonos de Carbono, esto abre una dimension nueva para financiar proyectos por medio de estos bonos que pueden negociarse a nivel internacional; por medio de la Produccion Limpia de energia se puede estimular inversion y ganancias para proyectos que contribuyan al

  17. Economic and environmental advantages for the use of the industrial potential of cogeneration in Mexico City; Ventajas economicas y ambientales para el aprovechamiento del potencial industrial de cogeneracion en Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Leon de los Santos, G. [Division de Estudios de Posgrado, Facultad de Ingenieria, UNAM, Mexico, D.F. (Mexico)

    2003-03-01

    Mexico has an industrial cogeneration potential very important, not evaluated or projected within its historical growth. The objective of the present work consist of studying the industrial cogeneration potential that exists in Mexico, as well as the economic and environmental savings that it would be achieved if one took advantage of this potential. As a result of the present work we can conclude that the cogeneration in Mexico offers a theoretical potential middle of 28,000 MWe to the 2007. It is estimated an economic saving potential in the construction of generation infrastructure electrical, for the high stage of its utilization of almost 7000 million of dollars and a stage of emissions reduction of 2007- 21.37% instead of 2007- 0.35% for the industrial sector of the country. These prognoses can be obtained, as long as the government changes vision to handle this subject as a measure of energy saving, and substitute it by the policy of complement the development of the national electrical sector. [Spanish] Mexico tiene un importante potencial de cogeneracion industrial que no ha sido evaluado ni proyectado dentro de su crecimiento historico, por ello, el objetivo de este trabajo consiste en estudiar y conocer los ahorros economicos y ambientales que se podrian lograr si se aprovechara dicho potencial. Como resultado, se muestra que la cogeneracion en Mexico ofrece un potencial teorico medio de 28,000 MWe al ano 2007. Se estima tambien un ahorro economico en la construccion de infraestructura de generacion electrica para el escenario alto de su aprovechamiento de casi 7 000 mdd, y un escenario de reduccion de emisiones para el sector industrial del pais de un 0.35% a un 21.4% para el ano 2007. Estos pronosticos se pueden lograr, siempre y cuando el gobierno cambie la vision de manejar este tema como una medida de ahorro de energia, y la sustituya por la politica de complemento al desarrollo del sector electrico nacional.

  18. System of lower cogeneration in the cement industry; Sistema de cogeneracion inferior en la industria del cemento

    Energy Technology Data Exchange (ETDEWEB)

    Romero Paredes, H.; Vazquez, A; Ambriz, J. J.; Fosado, A.; Cedillo, D.; Sanchez, R. [Universidad Autonoma Metropolitana-Iztapalapa (Mexico)

    1999-07-01

    energeticos en la industria cementera representan entre el 30 y 60% de los costos de produccion, por lo que cualquier disminucion en su consumo, repercutira considerablemente en la productividad de la empresa. Para determinar la capacidad disponible de energia de desecho y establecer la dimension del sistema de cogeneracion se procedio primeramente a realizar los balances de materia y energia de un tren de produccion de cemento. Para la evaluacion y simulacion numerica se tomo un caso de estudio de una planta nacional. El analisis toma unicamente en cuenta el horno rotatorio, el precalcinador, el enfriador de gases o acondicionador, el enfriador del clinker y los separadores o recuperadores de polvo. En este estudio no se han considerado los molinos ni los sistemas electricos que operan en toda la planta. Los resultados muestran que existe en general un elevado potencial de cogeneracion pues en algunos casos las perdidas de calor pueden alcanzar hasta un 50% de la energia calorifica de entrada. La capacidad de generacion electrica mediante turbina de vapor al aprovechar una fraccion (del orden del 60%) del calor residual, puede ser entre 200 y 300 wats por kg. de clinker producido. En conclusion, al recuperar mediante intercambiadores de calor apropiados para cada uno de los equipos que se mencionaron la energia desperdiciada y una red de intercambio de calor optimizada mediante tecnicas modernas se puede generar una parte importante de la energia electrica que usa una planta cementera. El metodo empleado ha resultado muy atractivo y con la posibilidad de aplicarlo a cualquier planta cementera y asi evaluar los potenciales de cogeneracion de energia.

  19. Experiences of energy saving and co-generation projects; Experiencias de proyectos de ahorro de energia y cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Alvarez Barajas, Alberto [Heat and Power Systems, S.A. de C.V., Mexico D.F. (Mexico)

    2005-07-01

    In this document are presented the successful projects of energy saving and co-generation that Heat and Power Systems, S.A. de C.V. has made in diverse industries. The investment recovery periods have been smaller to 18 months. The projects have been made for different companies within Mexican Republic. [Spanish] Se presentan los casos exitosos de proyectos de ahorro de energia y cogeneracion que Heat and Power Systems, S.A. de C.V. ha realizado en diversas industrias cuyo periodo de recuperacion de la inversion han sido menores a 18 meses. Los proyectos han sido realizados para distintas empresas dentro de la Republica Mexicana.

  20. Procedure to determine the convenience of installing a co-generation system; Procedimiento para determinar la conveniencia de instalar un sistema de cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Colin Castellanos, Carlos; Acosta Torres, Aracely [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1993-12-31

    In this article a methodology for the selection of the co-generation system better adapted to the energy needs of an industry is presented, covering the following schemes: a) Satisfy the thermal demand and deliver to the net the excess electricity generated. b) Reduce the functioning level of the machine and produce electricity in accordance with the internal demand. The thermal demand will be satisfied, for instance, integrating a boiler in the cycle. c) Satisfy the electric demand and utilize only in part the available heat. d) Or else, reduce the operation level of the machine and produce only the necessary thermal energy, taking from the net the electric energy needed to cover the total demand. [Espanol] En este articulo se presenta una metodologia para la seleccion del sistema de cogeneracion que mejor se adapte a las necesidades energeticas de una industria, cubriendo los siguientes esquemas: a) Satisfacer la demanda termica y ceder a la red el exceso de energia electrica generada. b) Reducir el nivel de funcionamiento de la maquina y producir electricidad de acuerdo a la demanda interna. La demanda termica se cubrira, por ejemplo, integrando una caldera al ciclo. c) Satisfacer la demanda electrica y, utilizar solo en parte el calor disponible. d) O bien, reducir el nivel de operacion de la maquina y solo producir la energia termica necesaria, tomando de la red la energia electrica para cubrir la demanda total.

  1. Procedure to determine the convenience of installing a co-generation system; Procedimiento para determinar la conveniencia de instalar un sistema de cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Colin Castellanos, Carlos; Acosta Torres, Aracely [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1992-12-31

    In this article a methodology for the selection of the co-generation system better adapted to the energy needs of an industry is presented, covering the following schemes: a) Satisfy the thermal demand and deliver to the net the excess electricity generated. b) Reduce the functioning level of the machine and produce electricity in accordance with the internal demand. The thermal demand will be satisfied, for instance, integrating a boiler in the cycle. c) Satisfy the electric demand and utilize only in part the available heat. d) Or else, reduce the operation level of the machine and produce only the necessary thermal energy, taking from the net the electric energy needed to cover the total demand. [Espanol] En este articulo se presenta una metodologia para la seleccion del sistema de cogeneracion que mejor se adapte a las necesidades energeticas de una industria, cubriendo los siguientes esquemas: a) Satisfacer la demanda termica y ceder a la red el exceso de energia electrica generada. b) Reducir el nivel de funcionamiento de la maquina y producir electricidad de acuerdo a la demanda interna. La demanda termica se cubrira, por ejemplo, integrando una caldera al ciclo. c) Satisfacer la demanda electrica y, utilizar solo en parte el calor disponible. d) O bien, reducir el nivel de operacion de la maquina y solo producir la energia termica necesaria, tomando de la red la energia electrica para cubrir la demanda total.

  2. Evaluation of the national cogeneration potential as an option for the expansion of the national electric system; Evaluacion del potencial nacional de cogeneracion como opcion en la expansion del sistema electrico nacional

    Energy Technology Data Exchange (ETDEWEB)

    Nieva Gomez, Rolando; Hernandez Galicia, Julio Alberto [Instituto de Investigaciones Electricas, Cuernavaca (Mexico); Portes Mascorro, Enrique; Alvarez Chavez, Jose Maria [Comision Nacional para el Ahorro de Energia (CONAE), Mexico, D. F. (Mexico)

    1997-12-31

    The Comision Nacional para el Ahorro de Energia (CONAE) carried out a study to determine the amount of electric energy available by cogeneration in the industrial sector of our country. This study is based in a survey among the enterprises with the largest fuel consumption belonging to the industrial and commercial sectors and to the petrochemical branch of Petroleos Mexicanos (PEMEX). The results of the study are presented, which show the location of the cogeneration potential by the following estimates: a) The scenario under a potential of 5770 MW to generate annually 4.55 x 107 MWh of electric energy. b) The scenario with a potential of 10819 MW to generate annually 9.47 x 107 Mwh of electric energy [Espanol] La Comision Nacional para el Ahorro de Energia (CONAE) llevo acabo un estudio para determinar la cantidad de energia electrica disponible por cogeneracion en el sector industrial de nuestro pais. El estudio se fundamenta en una encuesta entre las empresas con mayor consumo de combustibles pertenecientes a los sectores industrial y comercial y al ramo petroquimico de Petroleos Mexicanos (PEMEX). Se presentan los resultados del estudio, el cual muestra la ubicacion del potencial de cogeneracion por los siguientes estimados: a) El escenario bajo con un potencial de 5770 MW para producir anualmente 4.55 x 107 Mwh de energia electrica. b) El escenario alto con un potencial de 10819 MW para producir anualmente 9.47 x 107 MWh de energia electrica

  3. Evaluation of the national cogeneration potential as an option for the expansion of the national electric system; Evaluacion del potencial nacional de cogeneracion como opcion en la expansion del sistema electrico nacional

    Energy Technology Data Exchange (ETDEWEB)

    Nieva Gomez, Rolando; Hernandez Galicia, Julio Alberto [Instituto de Investigaciones Electricas, Cuernavaca (Mexico); Portes Mascorro, Enrique; Alvarez Chavez, Jose Maria [Comision Nacional para el Ahorro de Energia (CONAE), Mexico, D. F. (Mexico)

    1998-12-31

    The Comision Nacional para el Ahorro de Energia (CONAE) carried out a study to determine the amount of electric energy available by cogeneration in the industrial sector of our country. This study is based in a survey among the enterprises with the largest fuel consumption belonging to the industrial and commercial sectors and to the petrochemical branch of Petroleos Mexicanos (PEMEX). The results of the study are presented, which show the location of the cogeneration potential by the following estimates: a) The scenario under a potential of 5770 MW to generate annually 4.55 x 107 MWh of electric energy. b) The scenario with a potential of 10819 MW to generate annually 9.47 x 107 Mwh of electric energy [Espanol] La Comision Nacional para el Ahorro de Energia (CONAE) llevo acabo un estudio para determinar la cantidad de energia electrica disponible por cogeneracion en el sector industrial de nuestro pais. El estudio se fundamenta en una encuesta entre las empresas con mayor consumo de combustibles pertenecientes a los sectores industrial y comercial y al ramo petroquimico de Petroleos Mexicanos (PEMEX). Se presentan los resultados del estudio, el cual muestra la ubicacion del potencial de cogeneracion por los siguientes estimados: a) El escenario bajo con un potencial de 5770 MW para producir anualmente 4.55 x 107 Mwh de energia electrica. b) El escenario alto con un potencial de 10819 MW para producir anualmente 9.47 x 107 MWh de energia electrica

  4. Co-generation project for the Combined Cycle Power Plant President Juarez Rosarito and a reverse osmosis desalting plant; Proyecto de cogeneracion para la planta de ciclo combinado Presidente Juarez Rosarito y una planta desaladora de osmosis inversa

    Energy Technology Data Exchange (ETDEWEB)

    Beltran Mora, Hector; Espindola Hernandez, Salvador [Universidad NAcional Autonoma de Mexico (UNAM), Mexico, D.F. (Mexico)

    2006-11-15

    cogeneracion: produccion de electricidad y agua. El programa de operacion de la central desaladora estara determinado por la demanda de energia de la central de ciclo combinado; la propuesta es que la planta desaladora opere en las horas de baja carga de la central y pare cuando sean las hora pico de demanda de energia electrica. Para que este estudio sea representativo se han tomado las curvas de demanda de energia de las unidades de ciclo combinado de la Central Presidente Juarez Rosarito de la Comision Federal de Electricidad (CFE) y datos actualizados de las plantas desaladoras de osmosis inversa que estan disponibles actualmente en el mercado. Como base del estudio se determinaran los costos nivelados tanto de la energia electrica generada por la central de ciclo combinado operando dentro y fuera de un esquema de cogeneracion y los costos nivelados para el agua producida por la planta de osmosis inversa bajo dos supuestos: el primero es comprando la energia electrica a CFE y el segundo considerando que la CFE es duena de la planta desaladora y por tanto el costo de energia electrica para desalar es cero. Este trabajo muestra los impactos economicos en los costos de la generacion de energia electrica y en los del agua desalada en un esquema de cogeneracion. Los resultados mostrados en este estudio pueden considerarse para la futura planeacion en la construccion de plantas desaladoras para abastecer de agua en las zona noroeste del pais donde se tienen serios problemas de desabasto.

  5. Cogeneration development in Mexico and its recent environmental impact; Desarrollo de la cogeneracion en Mexico y su reciente impacto ambiental

    Energy Technology Data Exchange (ETDEWEB)

    Elizalde Baltierra, Alberto; Garcia Pelaez, Juan [Universidad Nacional Autonoma de Mexico, Mexico, D. F. (Mexico)

    1996-12-31

    This paper presents the historical development of the electric auto supply systems and cogeneration in Mexico since 1942 to 1995 with the understanding that these are clearly differentiated and defined since 1992. Though it is not, but until 1994 when the first ex profeso permit for cogeneration was granted. It also develops a short prospective vision of the cogeneration in our country, as well as the fuel consumption employed by auto- supply systems during 1993. The pollution emissions to the environment are also analyzed and with it the environmental impact of these electric systems in this year. [Espanol] Se presenta el desarrollo historico de los sistemas de autoabastecimiento electrico y cogeneracion en Mexico desde 1942 a 1995, en el entendido que estos se diferencian y definen claramente desde 1992. Es, sin embargo, hasta 1994 cuando se otorgo el primer permiso ex profeso de cogeneracion. Se desarrolla, ademas, una breve vision prospectiva de la cogeneracion en nuestro pais. Asimismo, se analiza el consumo de combustibles empleados por los sistemas de autoabastecimiento en 1993. Se estiman las emisiones contaminantes a la atmosfera y con ello se valora el impacto ambiental de estos sistemas electricos en ese ano.

  6. Cogeneration development in Mexico and its recent environmental impact; Desarrollo de la cogeneracion en Mexico y su reciente impacto ambiental

    Energy Technology Data Exchange (ETDEWEB)

    Elizalde Baltierra, Alberto; Garcia Pelaez, Juan [Universidad Nacional Autonoma de Mexico, Mexico, D. F. (Mexico)

    1997-12-31

    This paper presents the historical development of the electric auto supply systems and cogeneration in Mexico since 1942 to 1995 with the understanding that these are clearly differentiated and defined since 1992. Though it is not, but until 1994 when the first ex profeso permit for cogeneration was granted. It also develops a short prospective vision of the cogeneration in our country, as well as the fuel consumption employed by auto- supply systems during 1993. The pollution emissions to the environment are also analyzed and with it the environmental impact of these electric systems in this year. [Espanol] Se presenta el desarrollo historico de los sistemas de autoabastecimiento electrico y cogeneracion en Mexico desde 1942 a 1995, en el entendido que estos se diferencian y definen claramente desde 1992. Es, sin embargo, hasta 1994 cuando se otorgo el primer permiso ex profeso de cogeneracion. Se desarrolla, ademas, una breve vision prospectiva de la cogeneracion en nuestro pais. Asimismo, se analiza el consumo de combustibles empleados por los sistemas de autoabastecimiento en 1993. Se estiman las emisiones contaminantes a la atmosfera y con ello se valora el impacto ambiental de estos sistemas electricos en ese ano.

  7. Acupuntura un tratamiento viable para las adicciones en Colombia

    Directory of Open Access Journals (Sweden)

    Hernán López Seuscún

    2013-07-01

    Los tratamientos con auriculoterapia, como el protocolo NADA (National Acupuncture Detoxification Association, son los métodos más usados para las adicciones en el mundo, y aunque no se ha logrado evidenciar su efectividad, por su costo, facilidad y el poco riesgo de efectos adversos se hace viable en un país con pocos recursos económicos como Colombia.

  8. Evaluation of avoided carbon dioxide emissions in cogeneration projects; Evaluacion de las emisiones evitadas de bioxido de carbono en proyectos de cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Flores Zamudio, Jesus Antonio; Fernandez Montiel, Manuel Francisco; Alcaraz Calderon, Agustin Moises [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)]. E-mail: jesus.flores@iie.org.mx; mffm@iie.org.mx; malcaraz@iie.org.mx

    2010-11-15

    In this paper, presents a methodology of how to calculate the emissions of CO{sub 2} (Carbon Dioxide) in cogeneration of plants for evaluate future cases with the type of fuel and fuel flow used in the plant. The methodology was in spreadsheets developed a series of stoichiometric balances. The methodology was done for three types of fossil fuels: solid, liquid and gas. The analysis is made only to the percentages of the items contained in the fuel flow automatically used and results in the combustion products in tons per hour. This method was compared with the results obtained in the software Thermoflow Inc. (Used in Gerencia de Procesos Termicos of Instituto de Investigaciones Electricas for evaluate various process systems that produce energy power) using different cogeneration systems, that is to say about the technology used emissions compared according to the amount of excess air for each type of technology and at one point before the gas cleaning systems. The results can be evaluated for emissions avoided through the fuel type used and developing a cogeneration plant compared to a conventional plant. [Spanish] En este articulo, se presenta una metodologia de como calcular las emisiones de CO{sub 2} (Bioxido de carbono) en plantas de cogeneracion, para evaluar casos a futuro por medio del tipo de combustible y flujo de combustible a utilizar en la planta. La metodologia se realizo en hojas de calculo, donde se desarrollaron una serie de balances estequiometricos. La metodologia se hizo para tres tipos de combustibles fosiles: solido, liquido y gas. El analisis se realiza con solo dar los porcentajes de los elementos que contiene el combustible y el flujo a utilizarse y automaticamente da como resultado los productos de la combustion en toneladas por hora. Esta metodologia se comparo con los resultados obtenidos en el software Thermoflow Inc. (Empleado en la Gerencia de Procesos Termicos del Instituto de Investigaciones Electricas para evaluar diversos

  9. El modelo de sistema viable: un instrumento para la organización efectiva

    Directory of Open Access Journals (Sweden)

    Norlando Sánchez Rueda

    2015-05-01

    Full Text Available RESUMEN En este ensayo se presenta una interpretación teórica del denominado Modelo de Sistema Viable (MSV, de Stafford Beer y su Potencial Aplicación en Tareas de Diagnóstico  y diseño empresarial, al igual que para Mejorar las capacidades Organizacionales de Auto- Regulación  y Auto- Organización. Se explica como el Modelo del Sistema Viable permite conocer e interpretar  los mecanismos de estabilidad y adaptabilidad de las organizaciones, pilares para el crecimiento de una verdadera organización Efectiva.

  10. Multimedia tutorial system to aid the technical training in energy cogeneration; Sistema tutorial multimedio para apoyar la capacitacion tecnica en cogeneracion de energia

    Energy Technology Data Exchange (ETDEWEB)

    Kemper Valverde, Nicolas; Lara Rosano, Felipe; Guerrero Briseno, Pedro [Laboratorio de Inteligencia Artificial, Instituto de Ingenieria UNAM, Mexico, D. F. (Mexico); Monedero De La Vega, Arturo F. [Seccion Ingenieria Energetica, DEPFI UNAM, Mexico, D. F. (Mexico)

    1994-12-31

    The incorporation of computer aided teaching technologies increases the efficiency in the transmission and acquisition of knowledge, allowing the handling of large information volumes, the knowledge homogenization among the students, the availability, the massive teaching and the permanent updating of the same. On the other hand, considering that all energy saving policy must be supported in a training and dissemination program of the respective energy technologies an immediate option is the use of computerized systems of teaching-learning. Precisely, in this paper, are presented the fundamental technical aspects for the development of a tutorial expert system, to aid the training in energy cogeneration, trying to integrate in the application, the usage of multimedia tools with the intelligent handling of the teaching-learning process through the inference machine of an expert system. [Espanol] La incorporacion de metodologias de ensenanza auxiliada por computadora incrementa la eficiencia en la transmision y adquisicion del conocimiento, permitiendo la manipulacion de grandes volumenes de informacion, la homogeneizacion del conocimiento en los educandos, la disponibilidad, masificacion y la permanente actualizacion del mismo. Por otro lado, considerando que toda politica de ahorro de energia debe estar soportada en un programa de capacitacion y de divulgacion de las tecnologias energeticas respectivas, una alternativa inmediata es el empleo de sistemas computarizados de ensenanza-aprendizaje. Precisamente, en este trabajo, se presenta los aspectos tecnicos fundamentales del desarrollo de un sistema experto tutorial para apoyar la capacitacion en cogeneracion de energia, tratando de integrar en la aplicacion, el empleo de las herramientas multimedios con el manejo inteligente del proceso de ensenanza-aprendizaje a traves de la maquina de inferencia de un sistema experto.

  11. Cogeneration in Mexico: Weaknesses, Threats, strengths and opportunities; Cogeneracion en Mexico: Debilidades, amenazas, fortalezas y oportunidades

    Energy Technology Data Exchange (ETDEWEB)

    Elizalde Baltierra, Alberto; Garcia Pelaez, Juan [Programa Universitario de Energia, UNAM (Mexico)

    1997-07-01

    In this paper the strategies are formulated and evaluated, taking into account the internal and external aspects of the cogeneration in Mexico that foment their development in the medium term (two or four years). In the formulation of strategies a matrix DAFO (Weaknesses, Threats, Strengths and Opportunities) is employed and in the evaluation is applied the procedure of analytical hierarchical structuring. The future on the cogeneration in the next years will depend on diverse factors, among which can be mentioned: the legal frame; the financial situation of the interested companies; the outsourcing of financing; the prices of the electricity and fuels, as well as their availability, among others. [Spanish] En este trabajo se formulan y evaluan estrategias que, tomando en cuenta los aspectos internos y externos de la cogeneracion en Mexico, fomenten su desarrollo en el mediano plazo (dos o cuatro anos). En la formulacion de estrategias se emplea la matriz DAFO (Debilidades, Amenazas, Fortalezas y Oportunidades) y en la evaluacion se aplica el procedimiento de jerarquizacion analitica. El futuro de la cogeneracion en los proximos anos dependera de diversos factores, entre los que se pueden mencionar: el marco legal; la situacion financiera de las empresas interesadas; las fuentes externas de financiamiento; los precios de la electricidad y de los combustibles, asi como su disponibilidad, entre otros.

  12. Utilization of agricultural sugar cane wastes as fuel in modern cogeneration systems applied in sugar cane mills; Aprovechamiento de los residuos agricolas caneras como combustible en sistemas de cogeneracion modernos aplicados a ingenios

    Energy Technology Data Exchange (ETDEWEB)

    Buendia Dominguez, Eduardo H. [Instituto de Investigaciones Electricas, Cuernavaca (Mexico); De Buen Rodriguez, Odon [Comision Nacional para el Ahorro de la Energia, Mexico, D. F. (Mexico)

    1998-12-31

    Considering the new legal frame on cogeneration in Mexico, the possibility of heat and electricity supply required by the sugar mills to be made by an independent cogenerator of the sugar mill, operating with the sugar cane bagasse and agricultural sugar cane wastes, has been evaluated. Such modern cogenerator would be characterized, besides operating in an independent way of the sugar mill, by the use of high efficiency equipment in its process of heat and electricity generation. In this sense the Comision Nacional para el Ahorro de Energia (CONAE) through its Coordination Program and the Instituto de Investigaciones Electricas (IIE) carried out a joint project to determine the technical and economical viability that the sugar industry maintains the present sugar production without the need of burning fuel oil, installing adjacent to every sugar mill, a modern cogeneration system, operated by independent producers, that using sugar cane bagasse and agricultural sugar cane wastes, allows the supply of all the steam and electricity required by the sugar mill, and additionally can add firm capacity and the supply of electric power to the national grid, during the grinding season as well as out of grinding season. [Espanol] En consideracion al nuevo marco juridico de la cogeneracion en Mexico se ha evaluado la posibilidad de que el suministro de calor y electricidad requerido por los ingenios azucareros sea proporcionado por un cogenerador independiente de la planta de azucar, el cual opere utilizando el bagazo y residuos agricolas caneras (biomasa canera). Dicho cogenerador moderno se caracterizaria, ademas de operar de manera independiente a la planta de azucar, por el uso de equipos de alta eficiencia en su proceso de produccion de calor y electricidad. En este sentido la Comision Nacional para el Ahorro de Energia (CONAE) a traves de la Coordinacion de Programacion y el Instituto de Investigaciones Electricas (IIE) realizaron un trabajo en conjunto para determinar

  13. Utilization of agricultural sugar cane wastes as fuel in modern cogeneration systems applied in sugar cane mills; Aprovechamiento de los residuos agricolas caneras como combustible en sistemas de cogeneracion modernos aplicados a ingenios

    Energy Technology Data Exchange (ETDEWEB)

    Buendia Dominguez, Eduardo H [Instituto de Investigaciones Electricas, Cuernavaca (Mexico); De Buen Rodriguez, Odon [Comision Nacional para el Ahorro de la Energia, Mexico, D. F. (Mexico)

    1999-12-31

    Considering the new legal frame on cogeneration in Mexico, the possibility of heat and electricity supply required by the sugar mills to be made by an independent cogenerator of the sugar mill, operating with the sugar cane bagasse and agricultural sugar cane wastes, has been evaluated. Such modern cogenerator would be characterized, besides operating in an independent way of the sugar mill, by the use of high efficiency equipment in its process of heat and electricity generation. In this sense the Comision Nacional para el Ahorro de Energia (CONAE) through its Coordination Program and the Instituto de Investigaciones Electricas (IIE) carried out a joint project to determine the technical and economical viability that the sugar industry maintains the present sugar production without the need of burning fuel oil, installing adjacent to every sugar mill, a modern cogeneration system, operated by independent producers, that using sugar cane bagasse and agricultural sugar cane wastes, allows the supply of all the steam and electricity required by the sugar mill, and additionally can add firm capacity and the supply of electric power to the national grid, during the grinding season as well as out of grinding season. [Espanol] En consideracion al nuevo marco juridico de la cogeneracion en Mexico se ha evaluado la posibilidad de que el suministro de calor y electricidad requerido por los ingenios azucareros sea proporcionado por un cogenerador independiente de la planta de azucar, el cual opere utilizando el bagazo y residuos agricolas caneras (biomasa canera). Dicho cogenerador moderno se caracterizaria, ademas de operar de manera independiente a la planta de azucar, por el uso de equipos de alta eficiencia en su proceso de produccion de calor y electricidad. En este sentido la Comision Nacional para el Ahorro de Energia (CONAE) a traves de la Coordinacion de Programacion y el Instituto de Investigaciones Electricas (IIE) realizaron un trabajo en conjunto para determinar

  14. Gas turbine application in power plants of Mexican refineries; Aplicacion de turbinas de gas en plantas de fuerza de refinerias mexicanas

    Energy Technology Data Exchange (ETDEWEB)

    Alcaraz Calderon, A. M.; Rodriguez Martinez, H.; Garcia Paredes, Job; Morales Guillen, Magdalena; Manzanares Papayanapolous, Emilio [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)]. E-mail: malcaraz@iie.org.mx; jhrm@iie.org.mx; jgarcia@iie.org.mx; mmmg@iie.org.mx; emp@iie.org.mx

    2010-11-15

    This article presents an overview of the application to have the gas turbine power generation systems, electric and steam for Mexican refineries. First there is a general description of the gas turbines and heat recovery (system components CHP) and CHP schemes are based on gas turbines capable of use in Mexican refineries. Subsequently referred to the characteristics of energy consumption (steam and electricity) from Mexican refineries and performs a technical analysis of CHP schemes based on gas turbines from the point of view of rates Q/W, power efficiencies and combined concluded that by their nature are very useful for refinery and gives some recommendations for improved implementation. [Spanish] En este articulo se presenta una vision general de la aplicacion que tienen las turbinas de gas en sistemas de generacion de energia electrica y vapor para refinerias mexicanas. Primeramente se da una descripcion general de las turbinas de gas y recuperadores de calor (componentes principales del sistema de cogeneracion) y se muestran los esquemas de cogeneracion basados en turbinas de gas susceptibles de utilizar en refinerias mexicanas. Posteriormente se mencionan las caracteristicas de consumos energeticos (vapor y energia electrica) de las refinerias mexicanas y se realiza un analisis tecnico de los esquemas de cogeneracion basado en turbinas de gas desde el punto de vista de tasas Q/W, eficiencias electricas y combinadas, concluyendose que por sus caracteristicas son muy utiles para refinerias y se dan algunas recomendaciones para su mejor aplicacion.

  15. Feasibility. Technical and financial concepts to determine the feasibility of a self-supplying project; Factibilidad. Conceptos tecnicos y financieros para determinar la factibilidad de un proyecto de autoabastecimiento

    Energy Technology Data Exchange (ETDEWEB)

    Vargas A, Enrique [IGSA S.A. de C.V. (Mexico)

    2005-07-01

    The co-generation is the transformation of an energy source to two or more forms. This document deals with the co-generation equipment: gas turbines, steam turbines and reciprocating motors. It also provides a comparison between turbines and motors altogether with an analysis determining how the savings in the co-generation are obtained -an analysis dealing with thermal balance, the advantage of the radiation, the heat of the oil, the heat of the coolant, the exhaust heat and the production cost of 1 kwh. [Spanish] La cogeneracion es la transformacion de una fuente de energia a dos o mas formas. En este documento se analizan los equipos de cogeneracion: turbinas de gas, turbinas de vapor y motores reciprocantes, de la comparacion entre turbinas y motores, de como se logran los ahorros en la cogeneracion, del balance termico, el aprovechamiento de la radiacion, del calor del aceite, del calor del refrigerante, del calor del escape y del costo en la produccion de 1 kwh.

  16. Opportunities for the operation of the Comision Federal de Electricidad`s fossil fuel power plant stations eventually to be retired as cogeneration units; Oportunidades de operar las centrales termoelectricas de la Comision Federal de Electricidad, eventualmente retirables como unidades de cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Buendia Dominguez, E. H. [Instituto de Investigaciones Electricas, Cuernavaca (Mexico); Merlos Rueda, R. M. [Programa de Ahorro de Energia para el Sector Electrico, Mexico, D. F. (Mexico)

    1995-12-31

    The Programa de Ahorro de Energia para el Sector Electrico (PAESE) (Program for Energy Conservation for the Electric Sector) in collaboration with the Instituto de Investigaciones Electricas (IIE), decided to evaluate technically and economically the possibilities of operating the Comision Federal de Electricidad`s Thermoelectric Power Stations (TPS), eventually to be retired as cogeneration units in supplying thermal energy to industry. The study was focussed to the Monterrey and San Jeronimo Power Stations, determining in a first stage, the optimum radius for handling and transporting steam and/or hot water and so to define the industries that potentially could obtain heat energy from the TPS, in this stage the thermal needs of the evaluated industries were determined (pressure, temperature and flow). In a second stage, the different arrangements to be made to operate the selected TPS, so as to be in position of satisfying such thermal energy demands were evaluated. This paper presents the results of this study. [Espanol] El Programa de Ahorro de Energia para el Sector Electrico (PAESE), en colaboracion con el Instituto de Investigaciones Electricas, decidieron evaluar tecnica y economicamente las posibilidades de operar las centrales termoelectricas (CT`s) de la Comision Federal de Electricidad, eventualmente retirables, como unidades de cogeneracion al ofrecer calor a la industria. El estudio se enfoco en las centrales de Monterrey y San Jeronimo, determinado en una primera etapa el radio optimo para el manejo y transporte de vapor y/o agua caliente y asi definir a las industrias que potencialmente podrian recibir calor de las CT`s, en esta etapa se determinaron las necesidades termicas de las industrias evaluadas (presion, temperatura y flujo). En una segunda etapa se evaluo los diferentes arreglos para operar las CT`s seleccionadas para que pudieran satisfacer dichas demandas termicas. En este trabajo se presentaron los resultados de dicho estudio.

  17. Opportunities for the operation of the Comision Federal de Electricidad`s fossil fuel power plant stations eventually to be retired as cogeneration units; Oportunidades de operar las centrales termoelectricas de la Comision Federal de Electricidad, eventualmente retirables como unidades de cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Buendia Dominguez, E H [Instituto de Investigaciones Electricas, Cuernavaca (Mexico); Merlos Rueda, R M [Programa de Ahorro de Energia para el Sector Electrico, Mexico, D. F. (Mexico)

    1996-12-31

    The Programa de Ahorro de Energia para el Sector Electrico (PAESE) (Program for Energy Conservation for the Electric Sector) in collaboration with the Instituto de Investigaciones Electricas (IIE), decided to evaluate technically and economically the possibilities of operating the Comision Federal de Electricidad`s Thermoelectric Power Stations (TPS), eventually to be retired as cogeneration units in supplying thermal energy to industry. The study was focussed to the Monterrey and San Jeronimo Power Stations, determining in a first stage, the optimum radius for handling and transporting steam and/or hot water and so to define the industries that potentially could obtain heat energy from the TPS, in this stage the thermal needs of the evaluated industries were determined (pressure, temperature and flow). In a second stage, the different arrangements to be made to operate the selected TPS, so as to be in position of satisfying such thermal energy demands were evaluated. This paper presents the results of this study. [Espanol] El Programa de Ahorro de Energia para el Sector Electrico (PAESE), en colaboracion con el Instituto de Investigaciones Electricas, decidieron evaluar tecnica y economicamente las posibilidades de operar las centrales termoelectricas (CT`s) de la Comision Federal de Electricidad, eventualmente retirables, como unidades de cogeneracion al ofrecer calor a la industria. El estudio se enfoco en las centrales de Monterrey y San Jeronimo, determinado en una primera etapa el radio optimo para el manejo y transporte de vapor y/o agua caliente y asi definir a las industrias que potencialmente podrian recibir calor de las CT`s, en esta etapa se determinaron las necesidades termicas de las industrias evaluadas (presion, temperatura y flujo). En una segunda etapa se evaluo los diferentes arreglos para operar las CT`s seleccionadas para que pudieran satisfacer dichas demandas termicas. En este trabajo se presentaron los resultados de dicho estudio.

  18. TECHOS VERDES: ¿UNA HERRAMIENTA VIABLE PARA LA GESTIÓN AMBIENTAL EN EL SECTOR HOTELERO DEL RODADERO, SANTA MARTA?

    Directory of Open Access Journals (Sweden)

    ZIELINSKI SEWERYN

    2012-07-01

    Full Text Available El acelerado desarrollo urbano genera una serie de problemas no sólo sociales y económicos, sino también ambientales. Los techos verdes son unas de las nuevas tecnologías que pueden ser utilizadas como herramientas para la gestión ambiental en los edificios. Sin embargo, existen muchas barreras que impiden su implementación a gran escala. Este artículo hace una revisión profunda de las experiencias y resultados de numerosas investigaciones en el tema, destacando los beneficios de los techos verdes y mostrando las barreras para la implementación de esta tecnología en el sector hotelero del Rodadero, Santa Marta. La metodología consistió en la revisión de resultados de investigaciones llevadas a cabo en diferentes ciudades del mundo, haciendo énfasis en las realizadas en condiciones climáticas similares a la zona de estudio. Adicionalmente, se aplicaron encuestas a los gerentes de 18 hoteles en El Rodadero con el fin de conocer su opinión acerca del tema. Como resultado, se concluyó que los techos verdes pueden ser herramientas muy efectivas para la gestión ambiental si son implementados a gran escala. Sin embargo, en el caso de El Rodadero, ésta es una opción poco viable, aunque existe un interés de implementar proyectos pilotos que podrían disipar la desconfianza de los empresarios.

  19. On-Chip Dielectrophoretic Separation and Concentration of Viable, Non-Viable and Viable but Not Culturable (VBNC) Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Packard, M M; Shusteff, M; Alocilja, E C

    2012-04-12

    Although bacterial culture remains the gold standard for detection of viable bacteria in environmental specimens, the typical time requirement of twenty-four hours can delay and even jeopardize appropriate public health intervention. In addition, culture is incapable of detecting viable but not culturable (VBNC) species. Conversely, nucleic acid and antibody-based methods greatly decrease time to detection but rarely characterize viability of the bacteria detected. Through selection by membrane permeability, the method described in this work employs positive dielectrophoresis (pDEP) for separation and purification of viable and VBNC species from water and allows concentration of bacteria for downstream applications.

  20. Proceedings of the 7 Annual conference of the Mexican association for the energy economy. The energy sector in Mexico: challenges and solutions. [Selected papers]; 7. Conferencia anual de la asociacion mexicana para la economia energetica. El sector energia en Mexico: retos y soluciones

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    The Mexican Association for the Energy Economy (AMEE) had its VII annual conference, The energy sector in Mexico: challenges and solutions, in October, 2004. Some of the expounded topics are: technical and environmental aspects of the combined cycle power stations; analysis of the generation of electric power across biogas from sanitary landfills in Mexico; cogeneration in large power plants; methodologies for the calculation of relative efficiency: description and application of natural gas distributors in Latin American and others. [Spanish] La Asociacion Mexicana para la Economia Energetica A. C. (AMEE), llevo acabo su VII conferencia anual El sector energia en Mexico: retos y soluciones, en octubre del 2004. Algunos de los temas tratados son: Aspectos tecnicos y medioambientales de las centrales de ciclo combinado con gasificacion integrada; Analisis de la generacion de energia electrica a traves de biogas procedente de rellenos sanitarios en Mexico; Cogeneracion en grandes centrales de proceso; Metodologias para el calculo de eficiencia relativa: descripcion y aplicacion para distribuidores de gas natural en Latinoamerica y otros.

  1. Separation of viable and non-viable tomato (Solanum lycopersicum L.) seeds using single seed near-infrared spectroscopy

    DEFF Research Database (Denmark)

    Shrestha, Santosh; Deleuran, Lise Christina; Gislum, René

    2017-01-01

    Single seed near-infrared (NIR) spectroscopy is a non-destructive technology commonly used for predicting lipids, proteins, carbohydrates and water content of agricultural products. The aim of the current study is to investigate the prospects of NIR spectroscopy in classifying viable and non...... identified as important for classification of viable and non-viable tomato seeds by iPLS-DA. The sensitivity i.e. ability to correctly identify the positive samples and specificity i.e. ability to reject the negative samples of the (iPLS-DA) model on identified spectral regions for prediction of viable......-viable tomato seeds of two cultivars using chemometrics. The data exploration were performed by principal component analysis (PCA). Subsequently, viable and non-viable seeds were classified by partial least squares-discriminant analysis (PLS-DA) and interval PLS-DA (iPLS-DA). The indication of clustering...

  2. The Conceptual Mechanism for Viable Organizational Learning Based on Complex System Theory and the Viable System Model

    Science.gov (United States)

    Sung, Dia; You, Yeongmahn; Song, Ji Hoon

    2008-01-01

    The purpose of this research is to explore the possibility of viable learning organizations based on identifying viable organizational learning mechanisms. Two theoretical foundations, complex system theory and viable system theory, have been integrated to provide the rationale for building the sustainable organizational learning mechanism. The…

  3. Polymerase chain reaction-based discrimination of viable from non-viable Mycoplasma gallisepticum

    Directory of Open Access Journals (Sweden)

    Ching Giap Tan

    2014-09-01

    Full Text Available The present study was based on the reverse transcription polymerase chain reaction (RT-PCR of the 16S ribosomal nucleic acid (rRNA of Mycoplasma for detection of viable Mycoplasma gallisepticum. To determine the stability of M. gallisepticum 16S rRNA in vitro, three inactivation methods were used and the suspensions were stored at different temperatures. The 16S rRNA of M. gallisepticum was detected up to approximately 20–25 h at 37 °C, 22–25 h at 16 °C, and 23–27 h at 4 °C. The test, therefore, could detect viable or recently dead M. gallisepticum (< 20 h. The RT-PCR method was applied during an in vivo study of drug efficacy under experimental conditions, where commercial broiler-breeder eggs were inoculated with M. gallisepticum into the yolk. Hatched chicks that had been inoculated in ovo were treated with Macrolide 1. The method was then applied in a flock of day 0 chicks with naturally acquired vertical transmission of M. gallisepticum, treated with Macrolide 2. Swabs of the respiratory tract were obtained for PCR and RT-PCR evaluations to determine the viability of M. gallisepticum. This study proved that the combination of both PCR and RT-PCR enables detection and differentiation of viable from non-viable M. gallisepticum.

  4. Economically viable large-scale hydrogen liquefaction

    Science.gov (United States)

    Cardella, U.; Decker, L.; Klein, H.

    2017-02-01

    The liquid hydrogen demand, particularly driven by clean energy applications, will rise in the near future. As industrial large scale liquefiers will play a major role within the hydrogen supply chain, production capacity will have to increase by a multiple of today’s typical sizes. The main goal is to reduce the total cost of ownership for these plants by increasing energy efficiency with innovative and simple process designs, optimized in capital expenditure. New concepts must ensure a manageable plant complexity and flexible operability. In the phase of process development and selection, a dimensioning of key equipment for large scale liquefiers, such as turbines and compressors as well as heat exchangers, must be performed iteratively to ensure technological feasibility and maturity. Further critical aspects related to hydrogen liquefaction, e.g. fluid properties, ortho-para hydrogen conversion, and coldbox configuration, must be analysed in detail. This paper provides an overview on the approach, challenges and preliminary results in the development of efficient as well as economically viable concepts for large-scale hydrogen liquefaction.

  5. Managing Viable Knowledge

    NARCIS (Netherlands)

    Achterbergh, J.M.I.M.; Vriens, D.J.

    2002-01-01

    In this paper, Beer's Viable System Model (VSM) is applied to knowledge management. Based on the VSM, domains of knowledge are identified that an organization should possess to maintain its viability. The logic of the VSM is also used to support the diagnosis, design and implementation of the

  6. Alternative analysis to increase the power in combined-cycle power plants; Analisis de alternativas para el incremento de potencia en plantas termoelectricas de Ciclo Combinado

    Energy Technology Data Exchange (ETDEWEB)

    Pacheco Cruz, Hector; Arriola Medellin, Alejandro M. [Gerencia de Procesos Termicos, Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)]. E-mail: hpacheco@iie.org.mx; aarriola@iie.org.mx

    2010-11-15

    The electricity industry traditionally had two thermodynamic cycles for power generation: conventional steam turbine (Rankine cycle) used to supply a base load during the day, and gas turbines (Brayton cycle), for its speed response, normally used to cover peak loads. However, to provide variable peak loads, the gas turbine, as a volumetric machine is affected by the change in air density by changing the combustion temperature. This paper shows the scheme of integration of both systems, that it's known as combined cycle and the different options that would have these power plants, to maintain or increase their power in variable ambient conditions. It analyzes different options, such as: 1. Supplementary fire in the stove. 2. Air cooling intake in the gas turbine (evaporation system or mechanical system). 3. Steam injection in the combustion chamber. [Spanish] La industria electrica tradicionalmente a contado con dos ciclos termodinamicos para generacion electrica: las turbinas convencionales de vapor (ciclo de Rankine) se utilizan para suministrar una carga base durante el dia, y las turbinas de gas (ciclo de Brayton), por su rapidez de respuesta, se utilizan normalmente para cubrir las cargas pico. Sin embargo, para suministrar las cargas variables pico, la turbina a gas, por ser una maquina volumetrica, se ve afectada por el cambio de la densidad del aire de combustion al cambiar la temperatura ambiente. En este trabajo se muestra el esquema de integracion de ambos sistemas, en lo que se conoce como ciclo combinado y las diferentes opciones que tendrian estas plantas de generacion electrica para mantener o incrementar su potencia en condiciones ambiente variable. Para ello se analizan diferentes opciones, tales como: 1.- Combustion suplementaria en el recuperador de calor. 2.- Enfriamiento del aire de admision a la turbina de gas (mediante un sistema de evaporacion o mediante un sistema mecanico). 3.- Inyeccion de vapor a la camara de combustion. Palabras

  7. Enumeration of viable and non-viable larvated Ascaris eggs with quantitative PCR

    Science.gov (United States)

    Aims: The goal of the study was to further develop an incubation-qPCR method for quantifying viable Ascaris eggs. The specific objectives were to characterize the detection limit and number of template copies per egg, determine the specificity of the method, and test the method w...

  8. Propuesta de diseño de monitoreo ambiental microbiológico para diagnóstico de niveles de contaminación en áreas de procesamiento aséptico

    OpenAIRE

    Heidy Pérez; Vicente L. Sánchez

    2010-01-01

    El monitoreo ambiental está diseñado para demostrar el control de partículas viables y no viables en un área crítica, en este caso solo se hace referencia a las viables. Dentro de estas áreas se incluyen el flujo laminar, los laboratorios y otras áreas limpias. El ensayo de partículas viables se realiza para bacterias, levaduras y hongos. Con el objetivo de mantener las áreas seguras y en las condiciones requeridas, es preciso chequear el ambiente de las mismas mediante el monitoreo de las s...

  9. Propuesta metodológica para determinar la flexibilidad curricular en programas académicos soportada por software libre bajo la concepción del modelo de sistema viable

    Directory of Open Access Journals (Sweden)

    Luis Obeymar Estrada Sapuyes

    2015-10-01

    Full Text Available La educación es el motor del desarrollo de la sociedad. Potencializa las competencias profesionales y laborales de individuos capaces de hacer frente a las exigencias propias del fenómeno de la globalización. Por tanto, muchos países se han involucrado en la creación de sistemas de aseguramiento de la calidad educativa a fin de diseñar currículos más flexibles y abiertos a la sociedad del conocimiento que suplan las vastas necesidades de formación, oferten variedad de estrategias educativas, promuevan la movilidad académica e impulsen proyectos colaborativos de tipo inter o multidisciplinario. En este sentido, las instituciones educativas están llamadas a promover metodologías orientadas al estudio curricular que permitan el diagnóstico y fundamenten el planteamiento de planes de mejoramiento para el aseguramiento de la calidad. A partir de este proyecto, se formuló una metodología que permitió estudiar y determinar el grado de flexibilidad curricular con base en un prototipo de sistema de información desarrollado con software libre que permitió la gestión de indicadores y representó las diversas manifestaciones de flexibilidad. Por su parte, el Modelo del Sistema Viable proporcionó la fundamentación teórica que permitió concretar el estudio y el diagnóstico organizacional, e identificar las necesidades de información entre los subsistemas definidos, incluyendo el entorno. El prototipo del sistema de información fue diseñado de tal manera que los programas académicos de una institución de educación superior tuvieran la capacidad de estructurar varios estudios de flexibilidad curricular a lo largo del tiempo a fin de establecer los comparativos necesarios y ser un suministro sólido para futuras reformas curriculares y un soporte para los procesos de aseguramiento de la calidad. El estudio se aplicó al programa de Ingeniería de Sistemas de la Institución Universitaria Centro de Estudios Superiores María Goretti

  10. La defensa antioxidante en las plantas: Una herramienta clave para la fitorremediación

    OpenAIRE

    Peralta-Pérez, M. del R.; Volke-Sepúlveda, T.L.

    2012-01-01

    La fitorremediación es una alternativa viable para tratar suelos contaminados con metales/metaloides y compuestos orgánicos. Durante la última década, numerosos estudios han demostrado su potencial para remediar suelos; no obstante, para superar algunas de sus limitaciones es importante continuar realizando investigación. De acuerdo con las características del contaminante y sitio a tratar, la fitorremediación ocurre a través de diferentes procesos. Para contaminantes orgánicos, destacan la f...

  11. A combination of direct viable count and fluorescence in situ hybridization for specific enumeration of viable Lactobacillus delbrueckii subsp.bulgaricus and Streptococcus thermophilus.

    Science.gov (United States)

    García-Hernández, J; Moreno, Y; Amorocho, C M; Hernández, M

    2012-03-01

    We have developed a direct viable count (DVC)-FISH procedure for quickly and easily discriminating between viable and nonviable cells of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus strains, the traditional yogurt bacteria. direct viable count method has been modified and adapted for Lact. delbrueckii subsp. bulgaricus and Strep. thermophilus analysis by testing different times of incubation and concentrations of DNA-gyrase inhibitors. DVC procedure has been combined with fluorescent in situ hybridization (FISH) for the specific detection of viable cells of both bacteria with specific rRNA oligonucleotide probes (DVC-FISH). Of the four antibiotics tested (novobiocin, nalidixic acid, pipemidic acid and ciprofloxacin), novobiocin was the most effective for DVC method and the optimum incubation time was 7 h for both bacteria. The number of viable cells was obtained by the enumeration of specific hybridized cells that were elongated at least twice their original length for Lactobacillus and twice their original size for Streptococcus. This technique was successfully applied to detect viable cells in inoculated faeces. Results showed that this DVC-FISH procedure is a quick and culture-independent useful method to specifically detect viable Lact. delbrueckii subsp. bulgaricus and Strep. thermophilus in different samples, being applied for the first time to lactic acid bacteria. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.

  12. Predictors of viable germ cell tumor in postchemotherapeutic residual retroperitoneal masses

    Directory of Open Access Journals (Sweden)

    Khalid Al Othman

    2014-01-01

    Full Text Available Objective: The aim of this study was to identify predictors of viable germ cell tumor (GCT in postchemotherapeutic residual retroperitoneal masses. Materials and Methods: The pertinent clinical and pathologic data of 16 male patients who underwent postchemotherapeutic retroperitoneal lymph node dissection (PC-RPLND at King Faisal Specialist Hospital and Research Centre between 1994 and 2005 were reviewed retrospectively. It was found that all patients received cisplatin-based chemotherapy for advanced testicular GCT. Results: Out of the 16 male patients, 2 (13%, 8 (50%, and 6 (37% had viable GCT, fibrosis, and teratoma, respectively. Ten (10 of the patients with prechemotherapeutic S1 tumor markers did not have viable GCT, and two of the six patients who had prechemotherapeutic S2 tumor markers have viable GCT. All tumor marker levels normalized after chemotherapy even in patients with viable GCT. Four patients had vascular invasion without viable GCT. Furthermore, four patients had more than 60% embryonal elements in the original pathology, but only 1 had viable GCT at PC-RPLND. Four of the five patients with immature teratoma had teratoma at PC-RPLND but no viable GCT; however, out of the four patients with mature teratoma, one had viable GCT and two had teratoma at PC-RPLND. Of the two patients with viable GCT, one had 100% embryonal cancer in the original pathology, prechemotherapeutic S2 tumor markers, history of orchiopexy, and no vascular invasion; the other patient had yolk sac tumor with 25% embryonal elements and 40% teratoma in the original pathology, and prechemotherapeutic S2 tumor markers. Conclusion: None of the clinical or pathological parameters showed a strong correlation with the presence of viable GCT in PC-RPLND. However, patients with ≥S2 may be at higher risk to have viable GCT. Further studies are needed to clarify this.

  13. Grazing of particle-associated bacteria-an elimination of the non-viable fraction.

    Science.gov (United States)

    Gonsalves, Maria-Judith; Fernandes, Sheryl Oliveira; Priya, Madasamy Lakshmi; LokaBharathi, Ponnapakkam Adikesavan

    Quantification of bacteria being grazed by microzooplankton is gaining importance since they serve as energy subsidies for higher trophic levels which consequently influence fish production. Hence, grazing pressure on viable and non-viable fraction of free and particle-associated bacteria in a tropical estuary controlled mainly by protist grazers was estimated using the seawater dilution technique. In vitro incubations over a period of 42h showed that at the end of 24h, growth coefficient (k) of particle-associated bacteria was 9 times higher at 0.546 than that of free forms. Further, 'k' value of viable cells on particles was double that of free forms at 0.016 and 0.007, respectively. While bacteria associated with particles were grazed (coefficient of removal (g)=0.564), the free forms were relatively less grazed indicating that particle-associated bacteria were exposed to grazers in these waters. Among the viable and non-viable forms, 'g' of non-viable fraction (particle-associated bacteria=0.615, Free=0.0086) was much greater than the viable fraction (particle-associated bacteria=0.056, Free=0.068). Thus, grazing on viable cells was relatively low in both the free and attached states. These observations suggest that non-viable forms of particle-associated bacteria were more prone to grazing and were weeded out leaving the viable cells to replenish the bacterial standing stock. Particle colonization could thus be a temporary refuge for the "persistent variants" where the viable fraction multiply and release their progeny. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  14. Grazing of particle-associated bacteria-an elimination of the non-viable fraction

    Directory of Open Access Journals (Sweden)

    Maria-Judith Gonsalves

    Full Text Available Abstract Quantification of bacteria being grazed by microzooplankton is gaining importance since they serve as energy subsidies for higher trophic levels which consequently influence fish production. Hence, grazing pressure on viable and non-viable fraction of free and particle-associated bacteria in a tropical estuary controlled mainly by protist grazers was estimated using the seawater dilution technique. In vitro incubations over a period of 42 h showed that at the end of 24 h, growth coefficient (k of particle-associated bacteria was 9 times higher at 0.546 than that of free forms. Further, ‘k’ value of viable cells on particles was double that of free forms at 0.016 and 0.007, respectively. While bacteria associated with particles were grazed (coefficient of removal (g = 0.564, the free forms were relatively less grazed indicating that particle-associated bacteria were exposed to grazers in these waters. Among the viable and non-viable forms, ‘g’ of non-viable fraction (particle-associated bacteria = 0.615, Free = 0.0086 was much greater than the viable fraction (particle-associated bacteria = 0.056, Free = 0.068. Thus, grazing on viable cells was relatively low in both the free and attached states. These observations suggest that non-viable forms of particle-associated bacteria were more prone to grazing and were weeded out leaving the viable cells to replenish the bacterial standing stock. Particle colonization could thus be a temporary refuge for the “persistent variants” where the viable fraction multiply and release their progeny.

  15. Regulation of Viable and Optimal Cohorts

    Energy Technology Data Exchange (ETDEWEB)

    Aubin, Jean-Pierre, E-mail: aubin.jp@gmail.com [VIMADES (Viabilité, Marchés, Automatique, Décisions) (France)

    2015-10-15

    This study deals with the evolution of (scalar) attributes (resources or income in evolutionary demography or economics, position in traffic management, etc.) of a population of “mobiles” (economic agents, vehicles, etc.). The set of mobiles sharing the same attributes is regarded as an instantaneous cohort described by the number of its elements. The union of instantaneous cohorts during a mobile window between two attributes is a cohort. Given a measure defining the number of instantaneous cohorts, the accumulation of the mobile attributes on a evolving mobile window is the measure of the cohort on this temporal mobile window. Imposing accumulation constraints and departure conditions, this study is devoted to the regulation of the evolutions of the attributes which are1.viable in the sense that the accumulations constraints are satisfied at each instant;2.and, among them, optimal, in the sense that both the duration of the temporal mobile window is maximum and that the accumulation on this temporal mobile window is the largest viable one. This value is the “accumulation valuation” function. Viable and optimal evolutions under accumulation constraints are regulated by an “implicit Volterra integro-differential inclusion” built from the accumulation valuation function, solution to an Hamilton–Jacobi–Bellman partial differential equation under constraints which is constructed for this purpose.

  16. Asouzu's Complementary Ontology as a Foundation for a Viable ...

    African Journals Online (AJOL)

    This paper on “Asouzu's Complementary Ontology as a foundation for a viable Ethic of the Environment”, posits that an ethic of the environment can be seen as viable if it considers the whole of reality as ontologically relevant. This point of view would free environmental ethics of anthropocentric bias and its attendant ...

  17. PMA-Linked Fluorescence for Rapid Detection of Viable Bacterial Endospores

    Science.gov (United States)

    LaDuc, Myron T.; Venkateswaran, Kasthuri; Mohapatra, Bidyut

    2012-01-01

    The most common approach for assessing the abundance of viable bacterial endospores is the culture-based plating method. However, culture-based approaches are heavily biased and oftentimes incompatible with upstream sample processing strategies, which make viable cells/spores uncultivable. This shortcoming highlights the need for rapid molecular diagnostic tools to assess more accurately the abundance of viable spacecraft-associated microbiota, perhaps most importantly bacterial endospores. Propidium monoazide (PMA) has received a great deal of attention due to its ability to differentiate live, viable bacterial cells from dead ones. PMA gains access to the DNA of dead cells through compromised membranes. Once inside the cell, it intercalates and eventually covalently bonds with the double-helix structures upon photoactivation with visible light. The covalently bound DNA is significantly altered, and unavailable to downstream molecular-based manipulations and analyses. Microbiological samples can be treated with appropriate concentrations of PMA and exposed to visible light prior to undergoing total genomic DNA extraction, resulting in an extract comprised solely of DNA arising from viable cells. This ability to extract DNA selectively from living cells is extremely powerful, and bears great relevance to many microbiological arenas.

  18. Procesos para una Astronomía que le aporte a Colombia

    OpenAIRE

    Duque Escobar, Gonzalo

    2011-01-01

    Se ha validado el Plan Nacional de Desarrollo Tecnológico en Astronomía 2011-2030, para el Plan Estratégico 2009-2012 de la Comisión Colombiana del Espacio CCE, por el Grupo de Astronáutica, Astronomía y Medicina Aeroespacial. Esperamos que el grupo de astrónomos convocado, responsable de la investigación centrado en procesos científicos existentes y no en distractores, pueda señalar las acciones pertinentes e identificar los recursos necesarios, para hacer viable un desarrollo científico y t...

  19. UN PENETRÓMETRO DINÁMICO PARA EVALUAR LA RESISTENCIA MECÁNICA EN SUELOS FORESTALES

    OpenAIRE

    Irma Vásquez García; Armando Gómez Guerrero; Alejandro Velázquez Martínez; Arnulfo Aldrete; Aurelio M. Fierros-González

    2011-01-01

    Se diseñó un penetrómetro cónico de impacto para evaluar la resistencia mecánica de los suelos forestales. El equipo diseñado es ligero ya que sólo pesa 4.1 kg, preciso porque se validó en campo y laboratorio, portátil porque desensamblado se puede cargar en el chaleco de un operador de campo, y práctico porque la capacitación para su uso es sencilla y es adecuado para trabajos de inventarios forestales y de suelo. Los resultados mostraron que el uso del prototipo es viable en diferentes ecos...

  20. ESTUDIO DE FACTIBILIDAD PARA LA MANUFACTURA DE EMPUÑADURAS DE PET RECICLADO

    OpenAIRE

    Mariana Juárez N; María E. Santiago J; Jesús Vera M

    2011-01-01

    Este artículo, expone los resultados obtenidos de la investigación realizada, para proponer una nueva reutilización viable del plástico, el cual, se usa en las botellas hechas de Polietilentereftalato (PET). Este plástico cuenta con gran potencial de reciclado. Sin embargo, forma parte de las miles de toneladas que son dispuestas a los rellenos sanitarios, generando entre otras problemáticas, la necesidad de generar mayores espacios para la gestión de desechos, además del consumo de materias ...

  1. DESARROLLO DE UN SISTEMA DE INFORMACIÓN PARA LA EMPRESA DATECSA S.A

    Directory of Open Access Journals (Sweden)

    Nelson Javier Reyes

    2013-09-01

    Full Text Available En el siguiente documento se presenta un estudio, implementación y análisis de un sistema para administrar la información de la empresa Colombiana Datecsa S.A. Inicialmente, se tienen en cuenta los requerimientos de los usuarios del sistema, a partir de esto se selecciona la opción tecnológica más viable, para su posterior implementación. Finalmente, se analiza la seguridad del sistema desarrollado, lo que conlleva a demostrar su robustez.

  2. Ensayos en laboratorio para el control de floraciones algales mediante ultrasonido

    Directory of Open Access Journals (Sweden)

    Luis A. Kieffer

    2015-01-01

    Full Text Available El mayor desarrollo de Cianobacterias generado por la eutroficación de ambientes acuáticos produce, además de afloramientos y compuestos con olores y sabores desagradables, liberación de metabolitos tóxicos. El desarrollo excesivo de algas también es problema para aguas que se pueden utilizar para consumo humano o procesos industriales, pero el tratamiento para su eliminación lleva a aumentos de los costos operativos. En este trabajo se analiza la utilización en laboratorio del ultrasonido como alternativa viable para controlar el desarrollo de algas, en particular de Cianobacterias. Los resultados obtenidos (utilizando una frecuencia de 20 kHz y una potencia de 9 W muestran que los organismos pertenecientes a las Clases Chrysophyceae, Euglenophyceae y Chlorophyceae fueron eliminados a los 5 minutos de exposición, y Raphidiopsis mediterranea dentro de los primeros 10 minutos, en tanto que, luego de 60 minutos, la reducción fue del 98% para M. aeruginosa y del 99% para Dolichospermum spiroides.

  3. Coal liquefaction becomes viable

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-11-15

    In 2003 the May/June issue of CoalTrans International speculated that coal liquefaction would become viable due to falling coal prices. This has not proved the case but the sustained high oil price is sparking new interest. A survey by Energy Intelligence and Marketing Research during November 2005 revealed a growth in the number of projects under development or at the feasibility stage. The article reports projects in China, the USA, Australia, New Zealand, the Philippines and India. China is commissioning the first wave of large liquefaction plants. The key question is whether other countries, particularly the USA, will follow.

  4. Strengthening Agricultural Research Capacity for Viable Extension ...

    African Journals Online (AJOL)

    Strengthening Agricultural Research Capacity for Viable Extension Policies in Nigeria: An Exploration of Ricoeur's Hermeneutic Theory for Analysing Extension Research. ... Progressively more, researchers use hermeneutic philosophy to inform the conduct of interpretive research. Analogy between the philosophical ...

  5. Utilization of the computational technic for the promotion and diffusion of the energy conservation practice; Utilizacion de tecnicas computacionales para promocionar y difundir el ahorro de energia

    Energy Technology Data Exchange (ETDEWEB)

    Kemper Valverde, N. [Laboratorio de Inteligencia Artificial, Instituto de Ingenieria, UNAM, Mexico D. F. (Mexico)

    1995-12-31

    In this paper are presented the developments obtained through the application of the new computational technologies in the diffusion and promotion tasks of energy efficient use and conservation. The most relevant aspects are described of three multimedia courses that were designed in order to increase the possibilities of offering personal training in efficient energy technic, as well as to have on hand totally open consulting and interactive tools. The courses that are described are: ``Design of Energy Conservation Programs for the Industry``, ``Energy Conservation in Combustion Equipment`` and ``Energy Cogeneration Technologies.`` On another subject, an analysis is also made of the possibilities for the development and application of expert systems that advise and give support to the work of technicians and engineers dedicated to energy conservation. The main characteristics of an expert system to give support to the energy analysis of lighting industrial systems, malls, buildings and hotels are also described. [Espanol] En este trabajo se presentan los desarrollos obtenidos por la aplicacion de las tareas de difusion y promocion del uso eficiente y ahorro de energia. Se describen los aspectos mas relevantes de tres cursos multimedia que fueron disenados con el objeto de incrementar las posibilidades de brindar capacitacion individualizada en tecnicas energeticas eficientes, asi como de contar con herramientas de consulta totalmente abiertas e interactivas. Los cursos que se describen son: ``Diseno de Programas de Ahorro de Energia en la Industria,`` ``Ahorro de Energia en Equipos de Combustion`` y ``Tecnologias de Cogeneracion de Energia.`` Por otro lado, tambien se hace un analisis de las posibilidades para el desarrollo y aplicacion de sistemas expertos que asesoren y apoyen el trabajo de tecnicos e ingenieros dedicados al ahorro de energia. Se describen las caracteristicas principales de un sistema experto disenado para brindar apoyo en analisis energeticos

  6. Utilization of the computational technic for the promotion and diffusion of the energy conservation practice; Utilizacion de tecnicas computacionales para promocionar y difundir el ahorro de energia

    Energy Technology Data Exchange (ETDEWEB)

    Kemper Valverde, N [Laboratorio de Inteligencia Artificial, Instituto de Ingenieria, UNAM, Mexico D. F. (Mexico)

    1996-12-31

    In this paper are presented the developments obtained through the application of the new computational technologies in the diffusion and promotion tasks of energy efficient use and conservation. The most relevant aspects are described of three multimedia courses that were designed in order to increase the possibilities of offering personal training in efficient energy technic, as well as to have on hand totally open consulting and interactive tools. The courses that are described are: ``Design of Energy Conservation Programs for the Industry``, ``Energy Conservation in Combustion Equipment`` and ``Energy Cogeneration Technologies.`` On another subject, an analysis is also made of the possibilities for the development and application of expert systems that advise and give support to the work of technicians and engineers dedicated to energy conservation. The main characteristics of an expert system to give support to the energy analysis of lighting industrial systems, malls, buildings and hotels are also described. [Espanol] En este trabajo se presentan los desarrollos obtenidos por la aplicacion de las tareas de difusion y promocion del uso eficiente y ahorro de energia. Se describen los aspectos mas relevantes de tres cursos multimedia que fueron disenados con el objeto de incrementar las posibilidades de brindar capacitacion individualizada en tecnicas energeticas eficientes, asi como de contar con herramientas de consulta totalmente abiertas e interactivas. Los cursos que se describen son: ``Diseno de Programas de Ahorro de Energia en la Industria,`` ``Ahorro de Energia en Equipos de Combustion`` y ``Tecnologias de Cogeneracion de Energia.`` Por otro lado, tambien se hace un analisis de las posibilidades para el desarrollo y aplicacion de sistemas expertos que asesoren y apoyen el trabajo de tecnicos e ingenieros dedicados al ahorro de energia. Se describen las caracteristicas principales de un sistema experto disenado para brindar apoyo en analisis energeticos

  7. Investigation of Removal Capacities of Biofilters for Airborne Viable Micro-Organisms

    Science.gov (United States)

    Soret, Rémi; Fanlo, Jean-Louis; Malhautier, Luc; Geiger, Philippe; Bayle, Sandrine

    2018-01-01

    New emerging issues appears regarding the possible aerosolization of micro-organisms from biofilters to the ambient air. Traditional bioaerosol sampling and cultural methods used in literature offer relative efficiencies. In this study, a new method revolving around a particle counter capable of detecting total and viable particles in real time was used. This counter (BioTrak 9510-BD) uses laser-induced fluorescence (LIF) technology to determine the biological nature of the particle. The concentration of viable particles was measured on two semi-industrial pilot scale biofilters in order to estimate the Removal Efficiency in viable particles (REvp) in stable conditions and to examine the influence of pollutant feeding and relative humidification of the gaseous effluent on the REvp. The REvp of biofilters reached near 80% and highlighted both the stability of that removal and the statistical equivalence between two identical biofilters. Pollutant deprivation periods of 12 h, 48 h and 30 days were shown to have no influence on the biofilters’ removal capacity, demonstrating the robustness and adaptation capacities of the flora. In contrast, a 90-day famine period turned the biofilters into emitters of viable particles. Finally, the humidification of the effluent was shown to negatively influence the removal capacity for viable particles, as drying off the air was shown to increase the REvp from 60 to 85%. PMID:29562709

  8. Fondos de inversión para del desarrollo forestal

    Directory of Open Access Journals (Sweden)

    J. Carmen Ayala Sosa

    2001-01-01

    Full Text Available El financiamiento bancario para las actividades forestales es muy restringido, principalmente para las empresas del sector social, que no cuentan con garantías hipotecarias que respalden sus créditos, aún y cuando se trate de proyectos viables y rentables. Ante dicha situación, en el presente trabajo se propone constituir fondos que la banca tome como garantía en el otorgamiento de créditos, es decir, garantizar créditos con aportaciones en efectivo por parte de los solicitantes. Lo atractivo de este esquema es que las empresas que requieren financiamiento, con sólo reunir el equivalente al 30 % de sus necesidades, pueden tener acceso al crédito. Una ventaja más de la propuesta es que para la formación de fondos, pueden hacerse aportaciones por los participantes del proceso productivo, lo que beneficia a los solicitantes de financiamiento ya que no son los únicos que corren el riesgo de las inversiones.

  9. Spatiotemporal Dynamics of Total Viable Vibrio spp. in a NW Mediterranean Coastal Area.

    Science.gov (United States)

    Girard, Léa; Peuchet, Sébastien; Servais, Pierre; Henry, Annabelle; Charni-Ben-Tabassi, Nadine; Baudart, Julia

    2017-09-27

    A cellular approach combining Direct Viable Counting and Fluorescent In Situ Hybridization using a one-step multiple-probe technique and Solid Phase Cytometry (DVC-FISH-SPC) was developed to monitor total viable vibrios and cover the detection of a large diversity of vibrios. FISH combined three probes in the same assay and targeted sequences located at different positions on the 16S rRNA of Vibrio and Aliivibrio members. We performed a 10-month in situ study to investigate the weekly dynamics of viable vibrios relative to culturable counts at two northwestern Mediterranean coastal sites, and identified the key physicochemical factors for their occurrence in water using a multivariate analysis. Total viable and culturable cell counts showed the same temporal pattern during the warmer season, whereas the ratios between both methods were inverted during the colder seasons (<15°C), indicating that some of the vibrio community had entered into a viable but non-culturable (VBNC) state. We confirmed that Seawater Surface Temperature explained 51-62% of the total variance in culturable counts, and also showed that the occurrence of viable vibrios is controlled by two variables, pheopigment (15%) and phosphate (12%) concentrations, suggesting that other unidentified factors play a role in maintaining viability.

  10. 9 CFR 113.26 - Detection of viable bacteria and fungi except in live vaccine.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Detection of viable bacteria and fungi... VECTORS STANDARD REQUIREMENTS Standard Procedures § 113.26 Detection of viable bacteria and fungi except... required to be free of viable bacteria and fungi, they shall also be tested as prescribed in this section...

  11. Can Malin's gravitational-field equations be modified to obtain a viable theory of gravity to obtain a viable theory of gravity to obtain a viable theory of gravity

    International Nuclear Information System (INIS)

    Smalley, L.L.; Prestage, J.

    1976-01-01

    Malin's gravitational theory, which was recently shown by Lindblom and Nester to be incorrect, is modified by means of a recently proposed method for obtaining viable gravitational theories. The resulting self-consistent theory, which is in effect a Rastall-type modification of the Einstein theory, exhibits nonconservation of momentum, yet agrees with all experimental limits known to date within the PPN framework

  12. Monotone viable trajectories for functional differential inclusions

    Science.gov (United States)

    Haddad, Georges

    This paper is a study on functional differential inclusions with memory which represent the multivalued version of retarded functional differential equations. The main result gives a necessary and sufficient equations. The main result gives a necessary and sufficient condition ensuring the existence of viable trajectories; that means trajectories remaining in a given nonempty closed convex set defined by given constraints the system must satisfy to be viable. Some motivations for this paper can be found in control theory where F( t, φ) = { f( t, φ, u)} uɛU is the set of possible velocities of the system at time t, depending on the past history represented by the function φ and on a control u ranging over a set U of controls. Other motivations can be found in planning procedures in microeconomics and in biological evolutions where problems with memory do effectively appear in a multivalued version. All these models require viability constraints represented by a closed convex set.

  13. Viable Techniques, Leontief’s Closed Model, and Sraffa’s Subsistence Economies

    Directory of Open Access Journals (Sweden)

    Alberto Benítez

    2014-11-01

    Full Text Available This paper studies the production techniques employed in economies that reproduce themselves. Special attention is paid to the distinction usually made between those that do not produce a surplus and those that do, which are referred to as first and second class economies, respectively. Based on this, we present a new definition of viable economies and show that every viable economy of the second class can be represented as a viable economy of the first class under two different forms, Leontief‘s closed model and Sraffa’s subsistence economies. This allows us to present some remarks concerning the economic interpretation of the two models. On the one hand, we argue that the participation of each good in the production of every good can be considered as a normal characteristic of the first model and, on the other hand, we provide a justification for the same condition to be considered a characteristic of the second model. Furthermore, we discuss three definitions of viable techniques advanced by other authors and show that they differ from ours because they admit economies that do not reproduce themselves completely.

  14. Inmunopotenciadores para la acuicultura

    Directory of Open Access Journals (Sweden)

    Rocmira Pérez

    2014-04-01

    Full Text Available La acuicultura es una de las actividades económicas de mayor crecimiento para la producción de alimentos. Uno de sus principales retos es la obtención de grandes volúmenes de producción con la mayor calidad posible. Esto conlleva a una reducción de la aplicación de antibióticos y productos quimioterapéuticos. Una de las estrategias más prometedoras es la aplicación de inmunopotenciadores, principalmente en los cultivos intensivos. El objetivo de este trabajo fue revisar los principales inmunopotenciadores, así como las tendencias y retos de su uso mundial. Se resumen las particularidades moleculares y funcionales de los mismos y se hace énfasis en los más estudiados: levamisol, ß-glucanos, lipopolisacárido, vitamina C, extractos de plantas y hormonas. Todos estos compuestos de naturaleza heterogénea inciden mayoritariamente en los componentes de la inmunidad innata de los peces, fortaleciendo y potenciando la resistencia a enfermedades; adicionalmente algunos de ellos tienen funciones antiestrés y favorecen su crecimiento. Se concluye que los inmunopotenciadores constituyen una estrategia viable para reducir las pérdidas por problemas sanitarios en el sector de la acuicultura; pero aún quedan por solucionar aspectos como la vía de administración y la etapa de inmunización adecuada para cada especie y tipo de cultivo.

  15. CONTROL PREDICTIVO HIBRIDO PARA FLOTACION DE MINERALES

    OpenAIRE

    PUTZ DE LA FUENTE, EDUARDO IGNACIO

    2014-01-01

    La minería es el sector de mayor actividad en la economía nacional, posicionando a Chile como el mayor productor y exportador de cobre a nivel mundial. Sin embargo, aún existen grandes desafíos que buscan mitigar el aumento en los costos de la energía y la disminución de las leyes de mineral, así como la promoción de nuevas tecnologías. Bajo este contexto, las tecnologías de automatización se presentan como una alternativa viable y necesaria para aumentar la productividad y confiabil...

  16. Microcrustáceos y Vibrio cholerae O1 viable no cultivable (VNC: resultados en la Cuenca del Río Salí, Tucumán, Argentina Microcrustaceans and viable but nonculturable (VNC Vibrio cholerae O1: results in the Salí River basin, Tucumán, Argentina

    Directory of Open Access Journals (Sweden)

    Cecilia Locascio de Mitrovich

    2010-01-01

    Full Text Available Vibrio cholerae reside habitualmente en aguas marinas y continentales. Según las condiciones ambientales y los recursos le sean “favorables” o “desfavorables”, se generan estados viables cultivables (VC o viables no cultivables (VNC respectivamente y, bajo esta última forma sobrevive. Para abordar la problemática del cólera en la Cuenca del Río Salí (Tucumán, Argentina, se realizaron muestreos durante los años 2003-2005 donde se consideraron aspectos fisicos, químicos, biológicos y sanitarios. Para evaluar los probables reservorios del patógeno, se analizó el zooplancton del Río Salí (Canal Norte y Banda Río Salí y Río Lules. La mayor representatividad taxonómica la registraron los copépodos, especialmente Eucyclops neumani (Pesta, 1927, junto a Acanthocyclops robustus (Sars, 1863, Metacyclops sp., Paracyclops chiltoni y Notodiaptomus incompositus (Brian, 1925, además de algunos rotíferos y cladóceros como (Lecane sp., y (Brachionus sp., Moina sp. y Leydigia sp.. La frecuencia de ocurrencia fue baja y no superó el 25%. El Canal Norte fue ambiente más propicio por la riqueza específica, abundancia y constancia de la comunidad. Las variables fisicas y químicas asociadas al zooplancton coincidirían con los valores que por nuestros registros y los antecedentes, se conocen para el desarrollo del patógeno. En el período estival hubo coincidencia entre la presencia de la forma VNC de V. cholerae O1 (inmunofluorescencia con anticuerpos anti O1 y el desarrollo del zooplancton. Se observaron formas VNC sobre apéndices o estructuras de copépodos ciclopoideos y cladóceros quidóridos, reflejando probablemente afinidad con sustratos quitinosos.Vibrio cholerae habitually lives in marine and continental waters. According to "favourable" or "unfavourable" resources and environmental conditions, viable (VC or viable non-culturable (VNC states will be generated, surviving only the latter form. To address the problem of

  17. EDUCACIÓN PARA CIUDADANOS DEL MUNDOCON IDENTIDAD AFRODESCENDIENTE: CASOINSTITUCIÓN EDUCATIVA ANTONIA SANTOS,CARTAGENA DE INDIAS, COLOMBIA

    OpenAIRE

    Lago de Zota, Alejandrina; Lago de Fernández, Carmen; Lago de Vergara, Diana

    2012-01-01

    Colombia, nación pluriétnica y multicultural, adopta la Etnoeducación como desafío y apuesta a la autonomía, equidad y pertinencia. La educación nintercultural constituye un enfoque fundamental para la construcción de paz y convivencia ciudadana. Desde esta reflexión, el grupo de investigación RUECA de la Universidad de Cartagena - Colombia, comprometido con propuestas curriculares que hagan viable una educación intercultural, con identidad afrodescendiente para ciudadanos del mundo, realiza ...

  18. Renewable energies for power generation in Latin America: market, technologies and perspectives; Energias renovables para generacion de electricidad en America Latina: mercado, tecnologias y perspectivas

    Energy Technology Data Exchange (ETDEWEB)

    De Martino Jannuzzi, Gilberto [Universidade Estadual de Campinas, UNICAMP e International Energy Initiative (Brazil); De Buen Rodriguez, Odon [Energia, Tecnologia y Educacion, S.C., ENTE, S.C. (Mexico); Gorenstein Dedecca, Joao [International Energy Initiative, IEI (Brazil); Goncalves Nogueira, Larissa [International Energy Initiative, IEI (Brazil); Maia Gomes, Rodolfo Dourado [International Energy Initiative, IEI (Brazil); Navarro, Judith [Energia, Tecnologia y Educacion, S.C., ENTE, S.C. (Mexico)

    2010-07-01

    many environmental obstacles exist. Bolivia, Ecuador and Peru are the South American countries that still have the greatest availability of economically viable water resources to be exploited, since those countries do not even use 7% of the available potential. Venezuela and Paraguay stand out in South America as using more than 50% of the economically available potential for hydroelectric generation in the region. Geothermal energy is an important resource in Mexico and Central America, with an installed capacity of 965 MW and 502 MW in 2008, respectively. In those two cases, this is the primary source of power generation. Argentina's high-enthalpy fields are sufficient for geothermal use to generate energy, but there is no estimate of the generation potential. Chile is another country that tries to develop its geothermal potential. In 2009, the government of Chile requested bids for 20 contracts for geothermal energy exploitation. During the geothermal bidding process, 59 offers from exploitation projects were received and 9 companies were granted licenses for the 20 areas solicited. Biomass is an especially important energy resource in Brazil, where it has already surpassed CHP. It is also relevant in Argentina and Colombia, where it is associated with the alcohol-sugar industry and a growing interest in cogeneration systems. [Spanish] America Latina posee un rico patrimonio de recursos energeticos renovables, a pesar de que, historicamente, el aprovechamiento de estos recursos en la region se haya realizado a traves de grandes centrales de energia hidroelectrica. Sin embargo, hay un inmenso potencial de utilizacion mas amplia de nuevas fuentes de energia renovables: Pequenas Centrales Hidroelectricas (PCH), eolica, solar, geotermica, de acuerdo con lo que presenta en este informe. Considerando solamente las fuentes renovables utilizadas para la generacion de electricidad en los paises estudiados, se observa que dichas fuentes participan con entre el 2.5 y el 5

  19. Renewable energies for power generation in Latin America: market, technologies and perspectives; Energias renovables para generacion de electricidad en America Latina: mercado, tecnologias y perspectivas

    Energy Technology Data Exchange (ETDEWEB)

    De Martino Jannuzzi, Gilberto [Universidade Estadual de Campinas, UNICAMP e International Energy Initiative (Brazil); De Buen Rodriguez, Odon [Energia, Tecnologia y Educacion, S.C., ENTE, S.C. (Mexico); Gorenstein Dedecca, Joao [International Energy Initiative, IEI (Brazil); Goncalves Nogueira, Larissa [International Energy Initiative, IEI (Brazil); Maia Gomes, Rodolfo Dourado [International Energy Initiative, IEI (Brazil); Navarro, Judith [Energia, Tecnologia y Educacion, S.C., ENTE, S.C. (Mexico)

    2010-07-01

    many environmental obstacles exist. Bolivia, Ecuador and Peru are the South American countries that still have the greatest availability of economically viable water resources to be exploited, since those countries do not even use 7% of the available potential. Venezuela and Paraguay stand out in South America as using more than 50% of the economically available potential for hydroelectric generation in the region. Geothermal energy is an important resource in Mexico and Central America, with an installed capacity of 965 MW and 502 MW in 2008, respectively. In those two cases, this is the primary source of power generation. Argentina's high-enthalpy fields are sufficient for geothermal use to generate energy, but there is no estimate of the generation potential. Chile is another country that tries to develop its geothermal potential. In 2009, the government of Chile requested bids for 20 contracts for geothermal energy exploitation. During the geothermal bidding process, 59 offers from exploitation projects were received and 9 companies were granted licenses for the 20 areas solicited. Biomass is an especially important energy resource in Brazil, where it has already surpassed CHP. It is also relevant in Argentina and Colombia, where it is associated with the alcohol-sugar industry and a growing interest in cogeneration systems. [Spanish] America Latina posee un rico patrimonio de recursos energeticos renovables, a pesar de que, historicamente, el aprovechamiento de estos recursos en la region se haya realizado a traves de grandes centrales de energia hidroelectrica. Sin embargo, hay un inmenso potencial de utilizacion mas amplia de nuevas fuentes de energia renovables: Pequenas Centrales Hidroelectricas (PCH), eolica, solar, geotermica, de acuerdo con lo que presenta en este informe. Considerando solamente las fuentes renovables utilizadas para la generacion de electricidad en los paises estudiados, se observa que dichas fuentes participan con entre el 2.5 y el 5% de

  20. PMA-PhyloChip DNA Microarray to Elucidate Viable Microbial Community Structure

    Science.gov (United States)

    Venkateswaran, Kasthuri J.; Stam, Christina N.; Andersen, Gary L.; DeSantis, Todd

    2011-01-01

    Since the Viking missions in the mid-1970s, traditional culture-based methods have been used for microbial enumeration by various NASA programs. Viable microbes are of particular concern for spacecraft cleanliness, for forward contamination of extraterrestrial bodies (proliferation of microbes), and for crew health/safety (viable pathogenic microbes). However, a "true" estimation of viable microbial population and differentiation from their dead cells using the most sensitive molecular methods is a challenge, because of the stability of DNA from dead cells. The goal of this research is to evaluate a rapid and sensitive microbial detection concept that will selectively estimate viable microbes. Nucleic acid amplification approaches such as the polymerase chain reaction (PCR) have shown promise for reducing time to detection for a wide range of applications. The proposed method is based on the use of a fluorescent DNA intercalating agent, propidium monoazide (PMA), which can only penetrate the membrane of dead cells. The PMA-quenched reaction mixtures can be screened, where only the DNA from live cells will be available for subsequent PCR reaction and microarray detection, and be identified as part of the viable microbial community. An additional advantage of the proposed rapid method is that it will detect viable microbes and differentiate from dead cells in only a few hours, as opposed to less comprehensive culture-based assays, which take days to complete. This novel combination approach is called the PMA-Microarray method. DNA intercalating agents such as PMA have previously been used to selectively distinguish between viable and dead bacterial cells. Once in the cell, the dye intercalates with the DNA and, upon photolysis under visible light, produces stable DNA adducts. DNA cross-linked in this way is unavailable for PCR. Environmental samples suspected of containing a mixture of live and dead microbial cells/spores will be treated with PMA, and then incubated

  1. Association of viable Mycobacterium leprae with Type 1 reaction in leprosy.

    Science.gov (United States)

    Save, Mrudula Prakash; Dighe, Anju Rajaram; Natrajan, Mohan; Shetty, Vanaja Prabhakaran

    2016-03-01

    The working hypothesis is that, viable Mycobacterium leprae (M. leprae) play a crucial role in the precipitation of Type 1 reaction (T1R) in leprosy. A total of 165 new multibacillary patients were studied. To demonstrate presence of viable M. leprae in reactional lesion (T1R+), three tests were used concurrently viz. growth in the mouse foot pad (MFP), immunohistochemical detection of M. leprae secretory protein Ag85, and 16s rRNA--using in situ RT-PCR. Mirror biopsies and non reactional lesions served as controls (T1R-). A significantly higher proportion of lesion biopsy homogenates obtained at onset, from T1R(+) cases have shown unequivocal growth in MFP, proving the presence of viable bacteria, as compared to T1R(-) (P leprae is a component/prerequisite and the secretory protein Ag 85, might be the trigger for precipitation of T1R.

  2. Sycamore produces viable seed after six years

    Science.gov (United States)

    A. F. Ike

    1966-01-01

    In the early stages of any tree improvement program it is desirable to know how soon progenies of selected parents can themselves be included in a breeding program. How soon will they produce viable pollen and seed? In the case of sycamore (Platanus occidentalis L.), the information is meager: the Woody- Plant Seed Manual lists the minimum commercial seedbearing age...

  3. Issues of organizational cybernetics and viability beyond Beer's viable systems model

    Science.gov (United States)

    Nechansky, Helmut

    2013-11-01

    The paper starts summarizing the claims of Beer's viable systems model to identify five issues any viable organizations has to deal with in an unequivocal hierarchical structure of five interrelated systems. Then the evidence is introduced for additional issues and related viable structures of organizations, which deviate from Beer's model. These issues are: (1) the establishment and (2) evolution of an organization; (3) systems for independent top-down control (like "Six Sigma"); (4) systems for independent bottom-up correction of performance problems (like "Kaizen"), both working outside a hierarchical structure; (5) pull production systems ("Just in Time") and (6) systems for checks and balances of top-level power (like boards and shareholder meetings). Based on that an evolutionary approach to organizational cybernetics is outlined, addressing the establishment of organizations and possible courses of developments, including recent developments in quality and production engineering, as well as problems of setting and changing goal values determining organizational policies.

  4. Formas cocoides de Helicobacter pylori: viables o degenerativas

    Directory of Open Access Journals (Sweden)

    Felipe Cava

    2003-06-01

    Full Text Available De los trabajos presentados acerca de las formas cocoides de Helicobacter pylori se deduce una controversia mucho mayor que la resultante del mero estudio clínico de este microorganismo. Parece claro que existe una conversión tanto in vivo como in vitro de las formas espirales a las formas cocoides inducida por varios motivos, como cultivos prolongados, estrés físico y químico, y agentes antimicrobianos. En esta revisión repasamos los puntos de vista que han dividido a investigadores de esta área en dos grupos bien definidos: Los que consideran a estas formas cocoides como un producto no viable de degeneración celular y los que piensan que estas formas son estructuras viables,durmientes o de resistencia frente a condiciones ambientales adversas. Esta discrepancia conlleva a que interrogantes sobre la relación entre la transmisión de la enfermedad y estas formas cocoides permanezcan sin respuesta todavía.

  5. Conflictividad y modernidad: una ética de mínimos para la resolución de conflictos

    Directory of Open Access Journals (Sweden)

    Arturo Vallejos Romero

    Full Text Available El objetivo del presente artículo es confrontar algunas ideas que puedan ayudarnos a salvar los desacuerdos en sociedades pluralistas, conflictivas y complejas como América Latina. En específico, las ideas que serán vertidas y confrontadas en el texto iniciarán con una breve introducción, para luego exponer el pluralismo en nuestras sociedades actuales y cómo éste nos impele a buscar salidas viables para salvar nuestras diferencias. Posteriormente trataremos el tipo de consenso al que deberíamos adscribir y la opción del diálogo o pacto comunicativo para lograrlo. Tomaremos, para el apartado anterior, algunos conceptos de la Acción Comunicativa y una Ética discursiva, para luego abordar el andamiaje donde se deberían sustentar ciertos mínimos deseables para dar cuenta del disenso y/o el conflicto y la propuesta de algunos mínimos. Consecuentemente, expondremos el rol del Estado como garante del bien común, para finalmente esbozar algunas conclusiones.

  6. Gasification of heavy fuels to produce electrical energy and hydrogen; Gasificacion de combustibles pesados para producir energia electrica e hidrogeno

    Energy Technology Data Exchange (ETDEWEB)

    Vera Garcia, Oscar Alberto [Universidad Nacional Autonoma de Mexico(UNAM), Mexico, D.F. (Mexico)

    2006-11-15

    integrado (CCGI). El segundo, examina la incorporacion de un reactor shift en el cual el gas de sintesis se transforma en CO{sub 2} y H{sub 2} el cual se usa para mover la turbina a gas, adaptada para hidrogeno puro. Finalmente se estudia la cantidad de otros subproductos que se pueden obtener de estos ciclos de cogeneracion tales como CO{sub 2} para ser usado en recuperacion secundaria de pozos petroleros, N{sub 2} para ser usado en la industria de fertilizantes o en la propia produccion petrolera y H{sub 2} para ser usado en la industria petroquimica o en la generacion con celdas de combustible. Todos los casos son estudiados en forma cuantitativa, realizando los balances de masa y energia de cada uno de ellos. Para darle mas sentido practico a los calculos, se han tomado como base los datos tecnicos de la Central Valladolid de Comision Federal de Electricidad (CFE). Este articulo proporciona una idea basica, pero muy practica, para estimar el consumo de combustible de los diferentes modos de arreglo de una central CCGI, asi como los volumenes de los diferentes gases que se pueden producir y las modificaciones al tamano de los equipos que se requieren.

  7. Viable Mycobacterium avium ssp. paratuberculosis isolated from calf milk replacer.

    Science.gov (United States)

    Grant, Irene R; Foddai, Antonio C G; Tarrant, James C; Kunkel, Brenna; Hartmann, Faye A; McGuirk, Sheila; Hansen, Chungyi; Talaat, Adel M; Collins, Michael T

    2017-12-01

    When advising farmers on how to control Johne's disease in an infected herd, one of the main recommendations is to avoid feeding waste milk to calves and instead feed calf milk replacer (CMR). This advice is based on the assumption that CMR is free of viable Mycobacterium avium ssp. paratuberculosis (MAP) cells, an assumption that has not previously been challenged. We tested commercial CMR products (n = 83) obtained from dairy farms around the United States by the peptide-mediated magnetic separation (PMS)-phage assay, PMS followed by liquid culture (PMS-culture), and direct IS900 quantitative PCR (qPCR). Conventional microbiological analyses for total mesophilic bacterial counts, coliforms, Salmonella, coagulase-negative staphylococci, streptococci, nonhemolytic Corynebacterium spp., and Bacillus spp. were also performed to assess the overall microbiological quality of the CMR. Twenty-six (31.3%) of the 83 CMR samples showed evidence of the presence of MAP. Seventeen (20.5%) tested positive for viable MAP by the PMS-phage assay, with plaque counts ranging from 6 to 1,212 pfu/50 mL of reconstituted CMR (average 248.5 pfu/50 mL). Twelve (14.5%) CMR samples tested positive for viable MAP by PMS-culture; isolates from all 12 of these samples were subsequently confirmed by whole-genome sequencing to be different cattle strains of MAP. Seven (8.4%) CMR samples tested positive for MAP DNA by IS900 qPCR. Four CMR samples tested positive by both PMS-based tests and 5 CMR samples tested positive by IS900 qPCR plus one or other of the PMS-based tests, but only one CMR sample tested positive by all 3 MAP detection tests applied. All conventional microbiology results were within current standards for whole milk powders. A significant association existed between higher total bacterial counts and presence of viable MAP indicated by either of the PMS-based assays. This represents the first published report of the isolation of viable MAP from CMR. Our findings raise concerns

  8. Herramientas digitales de mercadeo, financieramente viables, para pequeñas y medianas empresas en la realidad empresarial colombiana

    OpenAIRE

    Martínez Ramírez, Jonathan Alexander; Medina Villegas, Laura

    2017-01-01

    Este artículo pretende dar una descripción general de las diferentes herramientas de mercadeo digital actualmente conocidas y proponer su aprovechamiento para la innovación, crecimiento y emprendimiento de las pequeñas y medianas empresas en un entorno tecnológico que avanza a pasos agigantados con retos y limitaciones financieras en contextos de gran complejidad organizacional y competitiva; tomando como base esta investigación, exploratoria y descriptiva, de las diferentes herramientas y es...

  9. Expression of proposed implantation marker genes CDX2 and HOXB7 in the blastocyst does not distinguish viable from non-viable human embryos

    DEFF Research Database (Denmark)

    Kirkegaard, Kirstine; Hindkjær, Johnny Juhl; Ingerslev, Hans Jakob

    2012-01-01

    expression differs between viable and non-viable embryos in both human and non-humans, suggesting transcriptome analysis of trophectoderm (TE) as a novel method of improving embryo selection. Potential candidate marker genes have been identified with array studies on animal blastocysts. The aim of this study...... was to investigate the expression of selected genes in human blastocysts in relation to the outcome of implantation. Materials and methods: Embryos from 10 oatients undergoing in vitro fertilization treatment were included in the project. A single blastocyst was chosen for biopsy on the morning of day 5 after oocyte...... of 15 key genes associated with developmental competence in animals were evaluated in high quality human embryos with monogenic or chromosomal disorders from a pre-implantation genetic disorder program. Triplicate cDNA amplifications for quantitative (q) RT-PCR were performed using pre-designed gene...

  10. Pertinencia de los postgrados para economistas en el Núcleo LUZ–COL

    Directory of Open Access Journals (Sweden)

    Ana Teresa Prieto Sánchez

    2002-01-01

    Full Text Available El presente artículo tiene como finalidad presentar los resultados obtenidos en un estudio de mercado, cuyo objetivo fue determinar la pertinencia de los postgrados para economistas en el Núcleo LUZ-COL. En dicho estudio se analizaron las expectativas, necesidades y percepciones de los usuarios potenciales del servicio de postgrado, considerando como usuarios potenciales (demanda a los entes empleadores de la COL, docentes de economía en el Núcleo, egresados del programa de economía en el Núcleo y estudiantes del último semestre de la carrera. Estos elementos sirvieron de base para conocer la pertinencia o no del postgrado. La metodología utilizada se fundamentó en una investigación descriptiva y cualitativa, en donde se utilizó el cuestionario como instrumento, para recabar la información primaria. Los resultados muestran una alta necesidad de los programas de postgrados para economistas y delimitan una demanda potencial considerable. El análisis permitió concluir que sí son pertinentes los postgrados para economistas en el Núcleo COL, en las áreas de gerencia de empresas, formulación y evaluación de proyectos, costos o finanzas; del tipo maestría o especialización profesional. En virtud de los resultados, se recomienda, desarrollar estrategias viables que permitan a los tomadores de decisión implementar el postgrado para economistas en el Núcleo.

  11. LA COOPERACIÓN: ESTRATEGIA PARA LA SOSTENIBILIDAD DE LOS RECURSOS // COOPERATION: STRATEGY FOR SUSTAINABILITY OF RESOURCES // COOPERAÇÃO: ESTRATÉGIA PARA A SUSTENTABILIDADE DOS RECURSOS

    Directory of Open Access Journals (Sweden)

    Clemencia Martínez Aldana

    2016-06-01

    Full Text Available Surge una gran inquietud en relación a cuál debe ser el principio de la estructuración de una organización, sino existe una base que subyace los cimientos de la estructura cuyo objetivo sea la sostenibilidad de los recursos. La idea no es simplemente definir la estructura corporativa, sino también buscar beneficios mutuos de forma colectiva que facilite la sostenibilidad y la optimización de los recursos de la organización, a partir de estrategias coherentes, pertinentes, viables y rigurosas. Para ello se plantean los postulados de críticos como Robert Axelrod, Thomas Hobbes, Michael Taylor, Thomas Schelling, Roy Behr y Elinor Ostrom, Garret Hardin, que sirvieron de base para la formulación teórica de la evolución en los sistemas de cooperación relacionados con los recursos. Se exponen y analizan los principios de: definición de límites, reglas y sanciones para los apropiadores de los recursos, arreglos de elección colectiva, y supervisión. Producto de los planteamientos expuestos, se infiere que para obtener beneficios relevantes, se hace necesario desarrollar modelos de autoadministración y autogestión colectiva.

  12. Skills training workshops as a viable strategy for improving ...

    African Journals Online (AJOL)

    Skills training workshops as a viable strategy for improving smallholder and cooperative agribusiness management: A case study of Vhembe District, Limpopo Province, South Africa. ... South African Journal of Agricultural Extension ... Empirical evidence from this study shows that six months after attending the workshops, ...

  13. Talons and beaks are viable but underutilized samples for detecting ...

    African Journals Online (AJOL)

    Talons and beaks are viable but underutilized samples for detecting organophosphorus and carbamate pesticide poisoning in raptors. Ngaio Richards, Irene Zorrilla, Joseph Lalah, Peter Otieno, Isabel Fernandez, Monica Calvino, Joaquin Garcia ...

  14. Quantitative assessment of viable Cryptosporidium parvum load in commercial oysters (Crassostrea virginica) in the Chesapeake Bay.

    Science.gov (United States)

    Graczyk, Thaddeus K; Lewis, Earl J; Glass, Gregory; Dasilva, Alexandre J; Tamang, Leena; Girouard, Autumn S; Curriero, Frank C

    2007-01-01

    The epidemiological importance of increasing reports worldwide on Cryptosporidium contamination of oysters remains unknown in relation to foodborne cryptosporidiosis. Thirty market-size oysters (Crassostrea virginica), collected from each of 53 commercial harvesting sites in Chesapeake Bay, MD, were quantitatively tested in groups of six for Cryptosporidium sp. oocysts by immunofluorescent antibody (IFA). After IFA analysis, the samples were retrospectively retested for viable Cryptosporidium parvum oocysts by combined fluorescent in situ hybridization (FISH) and IFA. The mean cumulative numbers of Cryptosporidium sp. oocysts in six oysters (overall, 42.1+/-4.1) were significantly higher than in the numbers of viable C. parvum oocysts (overall, 28.0+/-2.9). Of 265 oyster groups, 221 (83.4%) contained viable C. parvum oocysts, and overall, from 10-32% (mean, 23%) of the total viable oocysts were identified in the hemolymph as distinct from gill washings. The amount of viable C. parvum oocysts was not related to oyster size or to the level of fecal coliforms at the sampling site. This study demonstrated that, although oysters are frequently contaminated with oocysts, the levels of viable oocysts may be too low to cause infection in healthy individuals. FISH assay for identification can be retrospectively applied to properly stored samples.

  15. Solar System constraints on a cosmologically viable f(R) theory

    Energy Technology Data Exchange (ETDEWEB)

    Bisabr, Yousef, E-mail: y-bisabr@srttu.ed [Department of Physics, Shahid Rajaee Teacher Training University, Lavizan, Tehran 16788 (Iran, Islamic Republic of)

    2010-01-18

    Recently, a model f(R) theory is proposed (Miranda et al. (2009)) which is cosmologically viable and distinguishable from LAMBDACDM. We use chameleon mechanism to investigate viability of the model in terms of Solar System experiments.

  16. Cinética de desinfección para cinco desinfectantes utilizados en industria farmacéutica Disinfection kinetics of five disifectants used in pharmaceutical industry

    Directory of Open Access Journals (Sweden)

    Lena Carolina Echeverri Prieto

    2007-08-01

    Full Text Available Para evaluar la actividad desinfectante de cada agente químico se implementó una metodología cuantitativa por medio de la neutralización de estos con el medio Dey-Engley y recuperar las células viables después de cada ensayo y calcular la cinética de desinfección la cual es expresada como coeficiente de letalidad (k. Se observó que este valor era inversamente proporcional al tiempo de contacto para los tratamientos con B. subtilis frente a todos los desinfectantes, y para A. niger con el alcohol etílico al 70 %, el amonio cuaternario catiónico y el hipoclorito de sodio.To assess disinfectant activity from each chemical agent, a quantitative methodology was implemented by means of its neutralization with Dey-Engley medium, and thus to achieve recovery of viable cells after each assay and to estimate disinfection kynetics, which is expressed as lethal coefficient (k. It was observed that this value was proportionally inverse to contact time for treatments with B. subtilis versus all the disinfectants, and for A. niger with 70 % ethyl alcohol, ionic quaternary ammonium, and the sodium hypochlorite.

  17. Posgrado en Comunicaciones, para profesionales de otras disciplinas

    Directory of Open Access Journals (Sweden)

    José Manuel De Pablos

    2015-01-01

    Full Text Available En América Latina enfrentamos un doble problema: los comunicadores saben poco o casi nada de las ocupaciones de otros profesionales –médicos, ingenieros, empresarios, economistas- y éstos carecen de las nociones y destrezas para incorporar las comunicaciones eficazmente a su práctica profesional. Probablemente sea utópico intentar formar periodistas que se acerquen al modelo polivalente del intelectual renacentista. En cambio puede ser más viable y productivo hacer un buen comunicador de un médico o un ingeniero. Este modelo de actualización y especialización puede mejorar sustancialmente la calidad del periodismo y ampliar los horizontes de la formación en comunicaciones

  18. Solar System constraints on a cosmologically viable f(R) theory

    International Nuclear Information System (INIS)

    Bisabr, Yousef

    2010-01-01

    Recently, a model f(R) theory is proposed (Miranda et al. (2009)) which is cosmologically viable and distinguishable from ΛCDM. We use chameleon mechanism to investigate viability of the model in terms of Solar System experiments.

  19. Non-viable antagonist cells are associated with reduced biocontrol performance by viable cells of the yeast Papiliotrema flavescens against Fusarium head blight of wheat.

    Science.gov (United States)

    Microbially-based plant disease control products have achieved commercial market success, but the efficacy of such biocontrol products is sometimes deemed inconsistent. Improper processing of harvested microbial biomass or long-term storage can reduce the proportion of viable cells and necessitate t...

  20. Selective detection of viable seed-borne Acidovorax citrulli by real-time PCR with propidium monoazide.

    Science.gov (United States)

    Tian, Qian; Feng, Jian-Jun; Hu, Jie; Zhao, Wen-Jun

    2016-10-14

    In recent years, use of the DNA-intercalating dye propidium monoazide (PMA) in real-time PCR has been reported as a novel method to detect viable bacteria in different types of samples, such as food, environmental, and microbiological samples. In this study, viable cells of Acidovorax citrulli, the causal agent of bacterial seedling blight and fruit blotch, were selectively detected and differentiated from dead cells by real-time fluorescent polymerase chain reaction amplification after the bacterial solution was treated with the DNA-binding dye PMA. The primers and TaqMan probe were based on the A. citrulli genome (Aave_1909, Gene ID: 4669443) and were highly specific for A. citrulli. The detection threshold of this assay was 10 3 colony-forming units per mL (CFU/mL) in pure cell suspensions containing viable and dead cells and infected watermelon seeds. Application of this assay enables the selective detection of viable cells of A. citrulli and facilitates monitoring of the pathogen in watermelon and melon seeds.

  1. A viable real estate economy with disruption and blockchain

    NARCIS (Netherlands)

    Veuger, Jan

    2017-01-01

    Two titles in one cover. On page 56-112 there's the English version of the book: 'A viable real estate economy with disruption and blockchain. Does real estate still have the value that it had, or is the valuation of real estate going to change due to surprising products and services, innovative

  2. Promoting Women Participation in Aquaculture as a Viable Tool for ...

    African Journals Online (AJOL)

    Promoting Women Participation in Aquaculture as a Viable Tool for Poverty Alleviation in the Rural Areas of Nigeria. ... Open Access DOWNLOAD FULL TEXT ... a source of income, also the paper focus on the roles of women in aquaculture, ...

  3. The Medical Insurance for a New Generation: a viable answer for the health needs of Mexican children Seguro Médico para una Nueva Generación: una respuesta viable a las necesidades de salud de la niñez mexicana

    Directory of Open Access Journals (Sweden)

    Onofre Muñoz-Hernández

    2012-01-01

    Full Text Available OBJECTIVE: To describe the Medical Insurance for a New Generation (SMNG as key public policy aimed at improving health care services for children under the age of five years in Mexico. MATERIALS AND METHODS: This paper analyzes interrelated aspects of public policies to provide health care to Mexican children: a the demographic and health status of children, with emphasis on the wide disparities between children of varying demographics; b the relationship of the main functions of a health care system: financing, equity, access and quality, with the provision of health care for children; c the architecture of the Medical Insurance for a New Generation. CONCLUSION: SMNG is a program that can be instrumental in lowering infant mortality and alleviating the burden of families to care for sick children by improving access, quality of care and equity.OBJETIVO: Describir la importancia del Seguro Médico para una Nueva Generación (SMNG como un elemento clave de las políticas públicas mexicanas destinadas a mejorar los servicios de salud para los niños menores de cinco años. MATERIAL Y MÉTODOS: Este artículo analiza aspectos interrelacionados con la salud de los niños mexicanos: a las disparidades del estado de salud entre los niños con distintas características demográficas, b la relación de las funciones del sistema de salud: finaciamiento, equidad, acceso y calidad, con la provisión de servicios de salud para los niños, c El diseño del Seguro Médico para una Nueva Generación como herramienta para disminuir las brechas de desigualdad en la niñez mexicana. CONCLUSIÓN: El SMNG es un programa cuyo principal objetivo es fortalecer los servicios de salud desde el lado de la demanda. SMNG puede contribuir a reducir la morbilidad y mortalidad en los menores de cinco años y aliviar la carga de las familias para el cuidado de sus niños enfermos, mejorando el acceso, la calidad de los servicios de salud y la equidad.

  4. Entry of Vibrio harveyi and Vibrio fischeri into the viable but nonculturable state.

    Science.gov (United States)

    Ramaiah, N; Ravel, J; Straube, W L; Hill, R T; Colwell, R R

    2002-01-01

    Physiological responses of marine luminous bacteria, Vibrio harveyi (ATCC 14216) and V. fischeri (UM1373) to nutrient-limited normal strength (35 ppt iso-osmolarity) and low (10 ppt hypo-osmolarity) salinity conditions were determined. Plate counts, direct viable counts, actively respiring cell counts, nucleoid-containing cell counts, and total counts were determined. Vibrio harveyi incubated at 22 degrees C in nutrient-limited artificial seawater (ASW) became nonculturable after approximately 62 and 45 d in microcosms of 35 ppt and 10 ppt ASW, respectively. In contrast, V. fischeri became nonculturable at approximately 55 and 31 d in similar microcosms. Recovery of both culturability and luminescence of cells in the viable but nonculturable state was achieved by addition of nutrient broth or nutrient broth supplemented with a carbon source, including luminescence-stimulating compounds. Temperature upshift from 22 degrees C to 30 degrees C or 37 degrees C did not result in recovery from nonculturability. The study confirms entry of V. harveyi and V. fischeri into the viable but nonculturable state under low-nutrient conditions and demonstrates nutrient-dependent resuscitation from this state. This study confirms loss of luminescence of V. harveyi and V. fischeri on entry into the viable but nonculturable state and suggests that enumeration of luminescent cells in water samples may be a rapid method to deduce the nutrient status of a water sample.

  5. Cybernetically sound organizational structures II: Relating de Sitter's design theory to Beer's viable system model

    NARCIS (Netherlands)

    Achterbergh, J.M.I.M.; Vriens, D.J.

    2011-01-01

    - Purpose – The purpose of this paper is to show how the viable system model (VSM) and de Sitter's design theory can complement each other in the context of the diagnosis and design of viable organizations. - Design/methodology/approach – Key concepts from Beer's model and de Sitter's design theory

  6. Introduce of Viable But Nonculturable Bacteria

    Directory of Open Access Journals (Sweden)

    Mehdi Hassanshahian

    2008-03-01

    Full Text Available Viable-But-Nonculturable-State (VBNC is the condition in which bacteria fail to grow on their routine bacteriological media where they would normally grow and develop into colonies, but are still alive and capable of renewed metabolic activity. VBNC state is useful for evaluating public health and for ascertaining the sterility of drinking water, pharmaceuticals, and foodstuff. A number of bacteria, mostly pathogenic to humans, have been proved to enter into this state in response to natural stresses such as starvation, incubation out of optimum growth temperature, increased osmotic pressure, etc. Once in the VBNC state, they undergo various physiological, structural, and genetic alterations. These alterations result in reduced cell size, conversion from bacilli to coccid, thickened cell walls, and peptidoglycan gaining many cross links. Metabolic changes also occur that include reductions in growth, nutrient transport, and respiratory rate; biosynthesis of new protein, and ATP remaining at a constant level. It has been shown that in the VBNC state, some pathogens conserve their virulence properties. Gene expression continues in the VBNC cell. Nucleic acids remain intact in the early VBNC phase but they gradually undergo degradation with prolonged VBNC. Cytological methods such as direct viable count and reduction of tetrazolium salts, and molecular methods such as reverse transcription polymerase chain reaction and green fluorescent protein have been used for the study of VBNC. Resuscitation from VBNC state starts when the inducing factor(s is/are lifted. Factors that help the resuscitation of VBNC bacteria include addition of certain nutrients and chemicals, introduction of a few culturable cells into the VBNC cell population, and passage through the animal host. As virulence properties are sustained during the VBNC phase, special care must be paid when evaluating sterility of drinking water.

  7. Separation of viable lactic acid bacteria from fermented milk

    Directory of Open Access Journals (Sweden)

    Tomohiko Nishino

    2018-04-01

    Full Text Available Probiotics are live microorganisms that provide health benefits to humans. Some lactic acid bacteria (LAB are probiotic organisms used in the production of fermented foods, such as yogurt, cheese, and pickles. Given their widespread consumption, it is important to understand the physiological state of LAB in foods such as yogurt. However, this analysis is complicated, as it is difficult to separate the LAB from milk components such as solid curds, which prevent cell separation by dilution or centrifugation. In this study, we successfully separated viable LAB from yogurt by density gradient centrifugation. The recovery rate was >90 %, and separation was performed until the stationary phase. Recovered cells were observable by microscopy, meaning that morphological changes and cell viability could be directly detected at the single-cell level. The results indicate that viable LAB can be easily purified from fermented milk. We expect that this method will be a useful tool for the analysis of various aspects of probiotic cells, including their enzyme activity and protein expression. Keywords: Food analysis, Microbiology

  8. Preliminary applied study of assessment ischemic/viable myocardium by 99Tcm-HL91

    International Nuclear Information System (INIS)

    Liu Gang; Wu Hua

    2004-01-01

    Objective: To investigate the representation of 99 Tc m -HL91 in the ischemic myocardium, evaluate the diagnosis value of 99 Tc m -HL91 on hypoxic but viable myocardium. Methods: Six patients with cardiac infarction all underwent 99 Tc m -MIBI SPECT and 99 Tc m -HL91 SPECT. Average radioactivity of ischemic area and normal area were respectively obtained by ROI (2 x 2 pixels) on heart minor axis of images, And the radioactivity ratios of target (ischemic area)-to-non target(normal area)were calculated. Results: In image of 99 Tc m -HL91 SPECT, two patients who's radioactivity coloboma of 99 Tc m -MIBI image could be filled with 99 Tc m -HL91, four patients were not caught sight of obvious filling up. Conclusion 99 Tc m -HL91 can be selectively uptaken by ischemic and hypoxic but viable myocardium. it combination of 99 Tc m -MIBI SPECT may be good for accurate diagnosis and differentiation of viable myocardium. (authors)

  9. Generación de un método de expansión de consultas basado en ontologías para un sistema de recuperación de información

    OpenAIRE

    Rey, Martín; Kuna, Horacio Daniel; Martini, Esteban; Podkowa, Lucas; Pautsch, J. Germán A.; Zamudio, Eduardo

    2014-01-01

    Un Sistema de Recuperación de Información se compone a partir de diferentes módulos, uno de ellos es aquel que permite expandir las consultas ingresadas por el usuario a fin de ampliar el espectro de las búsquedas que se realicen. Existen numerosos métodos para la expansión de consultas, uno de ellos son las ontologías que, por su flexibilidad y capacidad para la representación del conocimiento, constituyen una alternativa viable para determinados contextos. Entre los problemas a los que se e...

  10. Studies on chlorophyll and viable mutations in green gram (Vigna radiata L. Wilczek) II: Response to mutagen

    Energy Technology Data Exchange (ETDEWEB)

    Krishnaswami, S; Rathinam, M [Tamil Nadu Agricultural Univ., Coimbatore (India). Dept. of Agricultural Botany

    1980-09-01

    The frequency and spectrum of chlorophyll and viable mutations in relation to type and dose of mutagen and cluster progenies were studied in four green gram cultivars viz., Kopergaon, Pusa Baisakhi, L. 24/2 and Sel. 122 subjected to two levels of EMS and gamma irradiation, severally and in conjunction. While chlorophyll mutations did not vary with the mutagen dose, viable mutations exhibited a direct relationship. Combinations of the mutagens were more effective in mutation induction. While no difference was manifested between the cluster families in respect of chlorophyll mutations, progenies of the second cluster recorded less viable mutations than either the first or the third. Viridis and xanthoviridis among chlorophyll mutations, and those affecting plant duration and stature among viable were more predominant.

  11. Evaluation of PCR and DNA hybridization protocols for detection of viable enterotoxigenic Clostridium perfringens in irradiated beef

    International Nuclear Information System (INIS)

    Baez, L.A.; Juneja, V.K.; Thayer, D.W.; Sackitey, S.

    1997-01-01

    The sensitivity of DNA hybridization and polymerase chain reaction (PCR), was evaluated in irradiated cooked and raw beef samples. A membrane-based colony hybridization assay and a PCR protocol, both with specificity for the enterotoxin A gene of Clostridium perfringens, were compared with viable plate counts. The results of the colony hybridization procedure were in agreement with viable plate counts for detection and enumeration of enterotoxigenic C. perfringens. The PCR procedure combined a 4 h enrichment followed by a nucleic acid extraction step and assessed the amplification of 183 and 750 base pair enterotoxin gene targets. Detection of C. perfringens by PCR did not show a reliable correlation with viable plate counts or the colony hybridization assay. C. perfringens killed by irradiation were not detected by the plate count or colony hybridization methods; however, killed cells were detected with the PCR technique. By relying on the growth of viable cells for detection and/or enumeration, the colony hybridization and plate count methods provided a direct correlation with the presence of viable bacteria

  12. Competencias docentes para enfrentar la sociedad del conocimiento

    Directory of Open Access Journals (Sweden)

    Yajaira del Carmen Oviedo G.

    2009-10-01

    Full Text Available Los avances científicos y la nueva economía global gestan la necesidad de adquirir nuevas competencias, en particular personales y profesionales, en los futuros ciudadanos y ciudadanas. Ante ello, es urgente la formación de actitudes, conocimientos y habilidades en el docente para afrontar exigencias y requerimientos de una sociedad del conocimiento que otorga un valor distinto a la información más allá de la mera transmisión. Esta reflexión de naturaleza documental hace un aporte a las instituciones formadoras del docente en este siglo XXI, ante el apremio de hacer frente a nuevos retos; asimismo, es viable como una alternativa al requisito de diseñar un perfil complejo que parta de la construcción, manejo y difusión de saberes y haceres vinculados al uso de las TIC. Desde este ámbito, el propósito es generar una visión holística de las perspectivas que constituyen la formación de este profesional (constructiva, reflexiva y humana para dar respuesta a los requerimientos de una sociedad que se transforma en todos los órdenes y, por ende, demanda nuevas formas de pensamiento para manejar el cúmulo de información a la cual está expuesta; implica el manejo y posicionamiento de estrategias y herramientas para mediar entre la tecnología y el estudiante. Estos aportes brindarán espacios para generar aproximaciones a un perfil del docente venezolano desde una dimensión ética, participativa, comprometida y consustanciada con su quehacer pedagógico mediado por las TIC. Se requiere una formación en las dimensiones del ser y el convivir, en las cuales la formación de principios como autonomía, libertad, respeto, responsabilidad, tolerancia, equidad, compromiso y solidaridad con sus pares, sean considerados rasgos fundamentales. Dejar de lado al profesional de la docencia desde concepciones mecanicistas, para entenderlo a partir de una visión integradora.

  13. Characterization of the Viable but Nonculturable (VBNC State in Saccharomyces cerevisiae.

    Directory of Open Access Journals (Sweden)

    Mohammad Salma

    Full Text Available The Viable But Non Culturable (VBNC state has been thoroughly studied in bacteria. In contrast, it has received much less attention in other microorganisms. However, it has been suggested that various yeast species occurring in wine may enter in VBNC following sulfite stress.In order to provide conclusive evidences for the existence of a VBNC state in yeast, the ability of Saccharomyces cerevisiae to enter into a VBNC state by applying sulfite stress was investigated. Viable populations were monitored by flow cytometry while culturable populations were followed by plating on culture medium. Twenty-four hours after the application of the stress, the comparison between the culturable population and the viable population demonstrated the presence of viable cells that were non culturable. In addition, removal of the stress by increasing the pH of the medium at different time intervals into the VBNC state allowed the VBNC S. cerevisiae cells to "resuscitate". The similarity between the cell cycle profiles of VBNC cells and cells exiting the VBNC state together with the generation rate of cells exiting VBNC state demonstrated the absence of cellular multiplication during the exit from the VBNC state. This provides evidence of a true VBNC state. To get further insight into the molecular mechanism pertaining to the VBNC state, we studied the involvement of the SSU1 gene, encoding a sulfite pump in S. cerevisiae. The physiological behavior of wild-type S. cerevisiae was compared to those of a recombinant strain overexpressing SSU1 and null Δssu1 mutant. Our results demonstrated that the SSU1 gene is only implicated in the first stages of sulfite resistance but not per se in the VBNC phenotype. Our study clearly demonstrated the existence of an SO2-induced VBNC state in S. cerevisiae and that the stress removal allows the "resuscitation" of VBNC cells during the VBNC state.

  14. Un paso adelante. Programa de inteligencia emocional y competencia social a través de las habilidades sociales para promover la convivencia y prevenir el abandono escolar temprano

    OpenAIRE

    Cano Calderón, Rafael

    2014-01-01

    Este trabajo presenta una propuesta de intervención socioeducativa dirigida a implantarse dentro de los centros educativos de ámbito Nacional mediante un programa de inteligencia emocional y competencia social a través de las habilidades sociales para promover la convivencia y prevenir el abandono escolar temprano. El Objetivo general de este programa es que desde la Educación Social se desarrolle una propuesta socioeducativa viable para prevenir el abandono escolar temprano y desarrollar ...

  15. The search for viable local government system in Nigeria: an ...

    African Journals Online (AJOL)

    The history of the Nigerian local government system has been one long episode of trails and errors aimed at achieving viable local government institution without much success. Local government in the country began its long series of reforms from the colonial period when the colonial government attempted to ...

  16. Viabilidad para pronósticos hidrológicos de niveles diarios, semanales y decadales en colombia

    Directory of Open Access Journals (Sweden)

    Efraín Antonio Domínguez Calle

    2010-05-01

    Full Text Available El presente artículo analiza y concluye sobre la viabilidad de pronósticos hidrológicos de niveles diarios, semanales y decadales en 20 estaciones hidrológicas de la red de monitoreo hidrometeorológico que soporta al Servicio de Alertas del Instituto de Hidrología, Meteorología y Estudios Ambientales – Ideam en Colombia (www.ideam.gov.co. Esta viabilidad se determina a través de un conjunto de criterios de desempeño ortogonales y para el presente estudio recomienda la aplicación de combinaciones lineales adaptativamente óptimas (CLAO como operador viable para la configuración de un sistema de pronóstico hidrológico en tiempo real de niveles diarios, semanales y decadales. En conclusión, se muestra que los pronósticos de niveles diarios, semanales y decadales tienen una viabilidad de pronóstico satisfactoria para el 70% de los casos estudiados.

  17. Viable Syntax: Rethinking Minimalist Architecture

    Directory of Open Access Journals (Sweden)

    Ken Safir

    2010-03-01

    Full Text Available Hauser et al. (2002 suggest that the human language faculty emerged as a genetic innovation in the form of what is called here a ‘keystone factor’—a single, simple, formal mental capability that, interacting with the pre-existing faculties of hominid ancestors, caused a cascade of effects resulting in the language faculty in modern humans. They take Merge to be the keystone factor, but instead it is posited here that Merge is the pre-existing mechanism of thought made viable by a principle that permits relations interpretable at the interfaces to be mapped onto c-command. The simplified minimalist architecture proposed here respects the keystone factor as closely as possible, but is justified on the basis of linguistic analyses it makes available, including a relativized intervention theory applicable across Case, scope, agreement, selection and linearization, a derivation of the A/A’-distinction from Case theory, and predictions such as why in situ wh-interpretation is island-insensitive, but susceptible to intervention effects.

  18. Glioma Surgical Aspirate: A Viable Source of Tumor Tissue for Experimental Research

    International Nuclear Information System (INIS)

    Day, Bryan W.; Stringer, Brett W.; Wilson, John; Jeffree, Rosalind L.; Jamieson, Paul R.

    2013-01-01

    Brain cancer research has been hampered by a paucity of viable clinical tissue of sufficient quality and quantity for experimental research. This has driven researchers to rely heavily on long term cultured cells which no longer represent the cancers from which they were derived. Resection of brain tumors, particularly at the interface between normal and tumorigenic tissue, can be carried out using an ultrasonic surgical aspirator (CUSA) that deposits liquid (blood and irrigation fluid) and resected tissue into a sterile bottle for disposal. To determine the utility of CUSA-derived glioma tissue for experimental research, we collected 48 CUSA specimen bottles from glioma patients and analyzed both the solid tissue fragments and dissociated tumor cells suspended in the liquid waste fraction. We investigated if these fractions would be useful for analyzing tumor heterogeneity, using IHC and multi-parameter flow cytometry; we also assessed culture generation and orthotopic xenograft potential. Both cell sources proved to be an abundant, highly viable source of live tumor cells for cytometric analysis, animal studies and in-vitro studies. Our findings demonstrate that CUSA tissue represents an abundant viable source to conduct experimental research and to carry out diagnostic analyses by flow cytometry or other molecular diagnostic procedures

  19. Study of potentials bio energy, aeolian, miniature hydraulic and solar in Mexico (Annexe 9 in 'A vision of year 2030 on the use of the renewable energies in Mexico'); Estudio de los potenciales bioenergetico, eolico, minihidraulico y solar en Mexico (Anexo 9 en 'Una vision al 2030 de la utilizacion de las energias renovables en Mexico')

    Energy Technology Data Exchange (ETDEWEB)

    Saldana Flores, Ricardo; Miranda Miranda, Ubaldo [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2005-08-15

    In this report we can observe maps and studies made about the evaluation of the bio energy potential of co-generation of electricity in the sugar industry and the sweepings, of the wind power potential in Latin America, the Caribbean and the Mexican Republic, of the miniature hydraulic potential and the hydro energy resources whereupon it counts country and, of the solar potential in which the OLADE presents/displays for Mexico the monthly maps of solar radiation maximum direct total and in Wm{sup 2}. [Spanish] En este reporte podemos observar mapas y estudios realizados acerca de la evaluacion del potencial bioenergetico de cogeneracion de electricidad en la industria azucarera y la basura, del potencial eoloenergetico en America Latina, el Caribe y la Republica Mexicana, del potencial minihidraulico y los recursos hidroenergeticos con que cuenta el pais y, del potencial solar en el cual la OLADE presenta para Mexico los mapas mensuales de radiacion solar maxima total y directa en W/m{sup 2}.

  20. PESO DE HOJAS COMO HERRAMIENTA PARA ESTIMAR EL ÁREA FOLIAR EN SOYA

    Directory of Open Access Journals (Sweden)

    Felipe Rafael Garcés Fiallos

    2011-07-01

    Full Text Available Cuantificaciones del área foliar en plantas son importantes en estudios de daños ocasionados por enfermedades, por lo tanto su determinación requiere el uso de equipamientos que no siempre se encuentran disponibles para todos. La utilización de determinaciones indirectas, como el peso de materia fresca o seca podría ayudar en este proceso. En este trabajo, se evaluó la relación entre el peso de hojas y área foliar, a partir de plantas recolectadas en el estadío R7.1, en 64 parcelas de campo con el cultivar de soja Nidera 5909 RG. El peso fresco fue medido luego de la colecta, el peso seco después de 48 horas de incubación a 65° C y el área foliar a través de un integralizador digital Licor. Fueron obtenidas ecuaciones significativas (p < 0.0001 e R2 de 0.74 a 0.97 para cada estrato y para la planta entera. Para la media de la planta, la relación de área foliar fue de y = 45.53 x + 19.03 para peso fresco e y = 176.17 x – 75.30 para peso seco. Esta herramienta se presenta potencialmente viable para estimar el área foliar de la planta. La utilización del peso seco es mas trabajosa, más no requiere pesaje de las hojas inmediatamente después de su colecta. La utilización futura de esta herramienta requiere estudios adicionales con otros cultivares a fin de verificarse si el comportamiento es similar.

  1. Axenic isolation of viable Giardia muris trophozoites.

    Science.gov (United States)

    Tillotson, K D; Buret, A; Olson, M E

    1991-06-01

    Large numbers of viable Giardia muris trophozoites were isolated from the duodenum of experimentally infected mice 6 days after inoculation with 1,000 G. muris cysts. A series of shaking, incubation, and washing steps in the presence of the broad-spectrum antibiotic piperacillin readily provided 4.9 +/- 1.5 x 10(5) G. muris trophozoites per mouse, free of detectable contaminant organisms. Anaerobic and microaerophilic culturing and scanning electron microscopy demonstrated axenic status and high purity of the isolates. The viability of trophozoites was 98 +/- 2%. Application of this technique should permit novel immunological and epidemiological analyses of G. muris infection and biochemical investigations of this protozoan parasite.

  2. Quantification of viable bacteria in wastewater treatment plants by using propidium monoazide combined with quantitative PCR (PMA-qPCR).

    Science.gov (United States)

    Li, Dan; Tong, Tiezheng; Zeng, Siyu; Lin, Yiwen; Wu, Shuxu; He, Miao

    2014-02-01

    The detection of viable bacteria in wastewater treatment plants (WWTPs) is very important for public health, as WWTPs are a medium with a high potential for waterborne disease transmission. The aim of this study was to use propidium monoazide (PMA) combined with the quantitative polymerase chain reaction (PMA-qPCR) to selectively detect and quantify viable bacteria cells in full-scale WWTPs in China. PMA was added to the concentrated WWTP samples at a final concentration of 100 micromol/L and the samples were incubated in the dark for 5 min, and then lighted for 4 min prior to DNA extraction and qPCR with specific primers for Escherichia coli and Enterococci, respectively. The results showed that PMA treatment removed more than 99% of DNA from non-viable cells in all the WWTP samples, while matrices in sludge samples markedly reduced the effectiveness of PMA treatment. Compared to qPCR, PMA-qPCR results were similar and highly linearly correlated to those obtained by culture assay, indicating that DNA from non-viable cells present in WWTP samples can be eliminated by PMA treatment, and that PMA-qPCR is a reliable method for detection of viable bacteria in environmental samples. This study demonstrated that PMA-qPCR is a rapid and selective detection method for viable bacteria in WWTP samples, and that WWTPs have an obvious function in removing both viable and non-viable bacteria. The results proved that PMA-qPCR is a promising detection method that has a high potential for application as a complementary method to the standard culture-based method in the future.

  3. Comparison of culture-based, vital stain and PMA-qPCR methods for the quantitative detection of viable hookworm ova.

    Science.gov (United States)

    Gyawali, P; Sidhu, J P S; Ahmed, W; Jagals, P; Toze, S

    2017-06-01

    Accurate quantitative measurement of viable hookworm ova from environmental samples is the key to controlling hookworm re-infections in the endemic regions. In this study, the accuracy of three quantitative detection methods [culture-based, vital stain and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR)] was evaluated by enumerating 1,000 ± 50 Ancylostoma caninum ova in the laboratory. The culture-based method was able to quantify an average of 397 ± 59 viable hookworm ova. Similarly, vital stain and PMA-qPCR methods quantified 644 ± 87 and 587 ± 91 viable ova, respectively. The numbers of viable ova estimated by the culture-based method were significantly (P methods. Therefore, both PMA-qPCR and vital stain methods appear to be suitable for the quantitative detection of viable hookworm ova. However, PMA-qPCR would be preferable over the vital stain method in scenarios where ova speciation is needed.

  4. PARA EVITAR LA CONTAMINACIÓN AMBIENTAL

    Directory of Open Access Journals (Sweden)

    F Pachón

    2005-01-01

    Full Text Available El presente artículo se enmarca dentro de la caracterización del sistema de producción bovina del municipio de Utica (Cundinamarca y dentro de los problemas más importantes que lo afectan. Como resultado, el estudio identificó la escasez de alimento de buena calidad en épocas secas como la principal limitante del productor bovino del municipio. Aprovechando una ventaja presente en la zona, como es la elaboración de panela (proceso a través del cual resultan algunos subproductos, como bagazo y melote, que pueden ser utilizados como alternativa alimenticia para los bovinos en cualquier época del año, se planteó un experimento en el que se elaboraron bloques multinutricionales con base en dichos subproductos. El experimento se adelantó en una finca de un productor y se utilizaron 57 novillos cebús con edades entre los 11 y los 24 meses y pesos entre los 124 y los 463 kilogramos. Los animales se dividieron dos grupos que se mantuvieron en pasturas no manejadas. Al grupo experimental se le ofrecieron adicionalmente bloques multinutricionales. Los animales fueron examinados para comprobar su estado fisiológico, con el fin de determinar posibles inconvenientes sanitarios que pudieran afectar el ensayo. Se realizaron pesajes para medir las ganancias de peso en los dos grupos. Los resultados mostraron promedios de ganancias diarias de peso similares a Pr < 5%, donde fueron ligeramente superiores las del grupo alimentado con los bloques multinutricionales (0,848 vs. 0,769 kg animal/día. Los aportes más importantes del experimento fueron, primero, brindar una opción para que el productor pueda alimentar a sus animales en épocas de escasez y evitar que tenga que venderlos a bajos precios. El segundo aporte fue abrir la posibilidad de usar los productos de la elaboración de la panela, que en muchas ocasiones se convierten en desechos que no son usados y que contaminan las fuentes de agua, de una forma económicamente viable.

  5. Propuesta del uso de una parte de los fondos Yasuní para la implementación de energía renovable en Ecuador al horizonte 2030.

    OpenAIRE

    Jerez Custode, Marcelo Alejandro

    2012-01-01

    El presente trabajo tiene como principal objetivo dar propuestas para la implementación de energía renovable en el Ecuador con la posibilidad de usar parte de los recursos económicos que obtendría el país por la iniciativa Yasuní ITT. Se espera que el presente estudio sea una guía o incentivo para la creación de sistemas de generación eléctrica usando recursos renovables, ampliando la visión de que en Ecuador la energía limpia es viable.

  6. Implementation and Evaluation of an Image Analysis System for Determining Viability of Pollen Grains in Temperate Rice Implementación y Evaluación de un Sistema de Análisis de Imagen para determinar la Viabilidad de Granos de Polen en Arroz Templado

    Directory of Open Access Journals (Sweden)

    Rodrigo Ortega B

    2011-03-01

    esterilidad floral (“vanazón”, reduciendo los rendimientos del cultivo. Conocer el número de granos de polen viable al momento de antésis permitiría determinar anticipadamente pérdidas de rendimiento por esterilidad floral; además, conocer el potencial de producción de polen viable sirve como una poderosa herramienta de selección en programas de mejoramiento y para determinar el efecto de algunos factores de manejo sobre la “vanazón”. El recuento manual de polen total y viable no es práctico produciendo además resultados muy variables. El presente trabajo tuvo como objetivos desarrollar y evaluar un sistema para el recuento de polen viable y total en anteras de arroz, en base a análisis de imágenes. Al momento de antésis se recolectaron tres panículas de cada una de las parcelas de dos experimentos de campo donde se evaluaron los efectos de diferentes factores de manejo (variedad, altura de agua, dosis de N y arreglo espacial sobre la esterilidad floral. Dos espiguillas y cuatro anteras se muestrearon desde cada panícula; los granos de polen fueron extraídos desde las anteras y se fotografiaron. Se realizaron recuentos manuales y digitales sobre las imágenes para determinar el número de polen total y viable. El recuento digital fue realizado con el software SigmaScan Pro, estableciendo algunos parámetros sobre la imagen antes del recuento. El recuento digital de polen alcanzó una buena precisión y exactitud y puede ser usado para estimar la viabilidad del polen y como variable respuesta para comparar tratamientos de manejo.

  7. Contracting of energy services: often a viable alternative

    International Nuclear Information System (INIS)

    Milic, M.; Bruendler, M.

    2001-01-01

    This article discusses the outsourcing of energy services as a viable alternative to the operation of own energy facilities. The advantages of contracting for enterprises wanting to focus on their core competencies and have their energy infrastructure financed, built, maintained and operated by a third party are discussed. Financial aspects are looked at and examples in connection with the calculation of actual energy costs are given. The article is concluded with tips on the evaluation of offers for contracting services and on the definition of ownership aspects and property boundaries

  8. Good practices in developing “on line” lessons: Indispensable elements for a systematized design Bonnes pratiques du développement de cours en ligne : les éléments indispensables d'une conception systématisée Buenas prácticas para desarrollar clases en línea: elementos indispensables para un diseño sistemático

    Directory of Open Access Journals (Sweden)

    Giancarlo De Agostini

    2011-12-01

    Full Text Available This article describes the construction of a lesson or course for the internet, emphasizing the characteristics in viable activities that need to be implemented for the course to be effective and complete: the Beginning stage, the Development stage and finally the End stage. We also consider different pedagogical techniques and their implications in preparing a course that will be delivered online, exclusively through the internet. At the beginning of the article, we discuss some successfull regional projects.Cet article décrit la construction d'un cours ou d'une formation destiné(e à être diffusé sur Internet, en mettant l'accent sur les caractéristiques des activités viables qui doivent être mises en place pour que la formation soit efficace et complète : la phase initiale, la phase de développement et enfin la phase finale. Nous étudions également différentes techniques pédagogiques et leurs implications sur la préparation d'un cours qui sera dispensé en ligne, exclusivement sur Internet. Au début de l'article, nous présentons quelques projets régionaux aboutis.este artículo describe la construcción de una clase o un curso para Internet, enfatizando las características mediante actividades viables que se deben implementar para que el curso sea eficaz y completo: la etapa de comienzo, la etapa de desarrollo, y por último la etapa final. También consideramos las distintas técnicas pedagógicas y sus implicaciones para la preparación de un curso que se dictará en línea, exclusivamente a través de Internet. Al comienzo del artículo, referimos algunos proyectos regionales exitosos.

  9. A multicenter study of viable PCR using propidium monoazide to detect Legionella in water samples.

    Science.gov (United States)

    Scaturro, Maria; Fontana, Stefano; Dell'eva, Italo; Helfer, Fabrizia; Marchio, Michele; Stefanetti, Maria Vittoria; Cavallaro, Mario; Miglietta, Marilena; Montagna, Maria Teresa; De Giglio, Osvalda; Cuna, Teresa; Chetti, Leonarda; Sabattini, Maria Antonietta Bucci; Carlotti, Michela; Viggiani, Mariagabriella; Stenico, Alberta; Romanin, Elisa; Bonanni, Emma; Ottaviano, Claudio; Franzin, Laura; Avanzini, Claudio; Demarie, Valerio; Corbella, Marta; Cambieri, Patrizia; Marone, Piero; Rota, Maria Cristina; Bella, Antonino; Ricci, Maria Luisa

    2016-07-01

    Legionella quantification in environmental samples is overestimated by qPCR. Combination with a viable dye, such as Propidium monoazide (PMA), could make qPCR (named then vPCR) very reliable. In this multicentre study 717 artificial water samples, spiked with fixed concentrations of Legionella and interfering bacterial flora, were analysed by qPCR, vPCR and culture and data were compared by statistical analysis. A heat-treatment at 55 °C for 10 minutes was also performed to obtain viable and not-viable bacteria. When data of vPCR were compared with those of culture and qPCR, statistical analysis showed significant differences (P 0.05). Overall this study provided a good experimental reproducibility of vPCR but also highlighted limits of PMA in the discriminating capability of dead and live bacteria, making vPCR not completely reliable. Copyright © 2016 Elsevier Inc. All rights reserved.

  10. Collateral circulation as a marker of the presence of viable myocardium in patients with recent myocardial infarction

    International Nuclear Information System (INIS)

    Fujita, M.; Ohno, A.; Wada, O.; Miwa, K.; Nozawa, T.; Yamanishi, K.; Sasayama, S.

    1991-01-01

    The relationship between the presence of viable myocardium and the extent of coronary collateral circulation to the infarct area was evaluated in 20 patients with a recent anterior myocardial infarction who had complete obstruction of the left anterior descending coronary artery. The viability of myocardial tissue was assessed by exercise thallium-201 myocardial scintigraphy, and the collateral circulation was angiographically evaluated by means of a collateral index ranging from 0 to 3. Patients were divided into two groups according to the presence (group 1, n = 10) or absence (group 2, n = 10) of viable myocardium in the perfusion territory of the infarct-related artery. The collateral index in group 1 was 2.5 ± 0.5 (SD), which was significantly higher than the 0.7 ± 0.8 in group 2. These findings indicate that the presence of ischemic but viable myocardium is intimately related to the development of collateral circulation in patients with myocardial infarction, and the existence of well-developed collateral channels predicts the presence of viable myocardium in the infarct area

  11. Fate of viable but non-culturable Listeria monocytogenes in pig manure microcosms

    Directory of Open Access Journals (Sweden)

    Jeremy eDesneux

    2016-03-01

    Full Text Available The fate of two strains of L. monocytogenes and their ability to become viable but non-culturable (VBNC was investigated in microcosms containing piggery effluents (two raw manures and two biologically treated manures stored for two months at 8°C and 20°C. Levels of L. monocytogenes were estimated using the culture method, qPCR, and propidium monoazide treatment combined with qPCR (qPCRPMA. The chemical composition and the microbial community structure of the manures were also analysed. The strains showed similar decline rates and persisted up to 63 days. At day zero, the percentage of VBNC cells among viable cells was higher in raw manures (81.5-94.8% than in treated manures (67.8-79.2%. The changes in their proportion over time depended on the temperature and on the type of effluent: the biggest increase was observed in treated manures at 20°C and the smallest increase in raw manures at 8°C. The chemical parameters had no influence on the behaviour of the strains, but decrease of the persistence of viable cells was associated with an increase in the microbial richness of the manures. This study demonstrated that storing manure altered the culturability of L. monocytogenes, which rapidly entered the VBNC state, and underlines the importance of including VBNC cells when estimating the persistence of the pathogens in farm effluents.

  12. Monitoring of viable airborne SARS virus in ambient air

    Science.gov (United States)

    Agranovski, Igor E.; Safatov, Alexander S.; Pyankov, Oleg V.; Sergeev, Alexander N.; Agafonov, Alexander P.; Ignatiev, Georgy M.; Ryabchikova, Elena I.; Borodulin, Alexander I.; Sergeev, Artemii A.; Doerr, Hans W.; Rabenau, Holger F.; Agranovski, Victoria

    Due to recent SARS related issues (Science 300 (5624) 1394; Nature 423 (2003) 240; Science 300 (5627) 1966), the development of reliable airborne virus monitoring procedures has become galvanized by an exceptional sense of urgency and is presently in a high demand (In: Cox, C.S., Wathers, C.M. (Eds.), Bioaerosols Handbook, Lewis Publishers, Boca Raton, FL, 1995, pp. 247-267). Based on engineering control method (Aerosol Science and Technology 31 (1999) 249; 35 (2001) 852), which was previously applied to the removal of particles from gas carriers, a new personal bioaerosol sampler has been developed. Contaminated air is bubbled through porous medium submerged into liquid and subsequently split into multitude of very small bubbles. The particulates are scavenged by these bubbles, and, thus, effectively removed. The current study explores its feasibility for monitoring of viable airborne SARS virus. It was found that the natural decay of such virus in the collection fluid was around 0.75 and 1.76 lg during 2 and 4 h of continuous operation, respectively. Theoretical microbial recovery rates of higher than 55 and 19% were calculated for 1 and 2 h of operation, respectively. Thus, the new sampling method of direct non-violent collection of viable airborne SARS virus into the appropriate liquid environment was found suitable for monitoring of such stress sensitive virus.

  13. ¿Es viable la ISO 14000 para el contexto colombiano?: una aproximación a partir de análisis de casos

    Directory of Open Access Journals (Sweden)

    Danilo Ariza Buenaventura

    2007-01-01

    Full Text Available El presente documento presenta los resultados fi nales de la ejecución de la investigación que en su desarrollo integró tres metodologías de investigación, análisis interpretativo de fuentes sobre organización y empresa a la luz del concepto de tecnorregión (Ariza, Gómez & León, 2006; la construcción e interpretación de una base datos sobre el proceso de difusión de la norma ISO 14001 (Ariza, Gómez & León, 2007, y fi nalmente, se hace una análisis de tres casos de empresas colombianas, en las que se ponen en juego la estructuras interpretativas construidas en documentos ateriere, constituyéndose en el eje central del presente documento. El documento concluye que pueden existir diversos niveles de asimilación cultural y predisposición organizacional, que hace de la ISO 14001 una norma no conveniente para todo tipo organización, en particular, se puede constituir en una fuerte barrera de entrada a los mercados internacionales para las empresas colombianas.

  14. Roots of success: cultivating viable community forestry

    Energy Technology Data Exchange (ETDEWEB)

    MacQueen, Duncan

    2009-05-15

    Is community forestry emerging from the shadows? The evidence shows that locally controlled enterprises can be economically viable, and often build on stronger social and environmental foundations than the big private-sector players. Certainly this is an industry in need of a shakeup. Many forests have become flashpoints where agro-industry, large-scale logging concerns and conservation interests clash, while forest-dependent communities are left out in the cold. Meanwhile, governments – driven by concerns over the climate impacts of deforestation – are having to gear up for legal, sustainable forestry production. Community forestry could be crucial to solving many of these challenges. By building on local core capabilities and developing strategic partnerships, they are forging key new business models that could transform the sector.

  15. Methodology for the energy analysis of compressed air systems; Metodologia para analisis energetico de sistemas de aire comprimido

    Energy Technology Data Exchange (ETDEWEB)

    Ramirez, T; Ambriz, J J; Romero, H [Universidad Autonoma Metropolitana-Iztapalapa, Mexico, D. F. (Mexico)

    1993-12-31

    In this paper a methodology for the diagnosis of compressed air systems, for the identification of the potential energy saving is presented. The methodology consists in detecting the largest number of possible ways of energy saving. For this purpose it is divided into three parts: 1. Compressed air generation. 2. Compressed air distribution. 3. Compressed air users. For each one of the parts, the type of information required to perform the diagnosis study, as well as the necessary measuring equipment needed, is indicated. Afterwards, the possible saving ways that can be found and the ones that can be feasible, are analyzed. [Espanol] En este trabajo se presenta una metodologia para diagnosticar sistemas de aire comprimido, para identificar los potenciales de ahorro de energia. La metodologia consiste en detectar el mayor numero de posibles medidas de ahorro, para lo cual se divide en tres partes: 1. Generacion de aire comprimido. 2. Distribucion del aire comprimido. 3 Usuarios de aire comprimido. Para cada una de las partes se indica el tipo de informacion requerida para realizar el estudio de diagnostico, asi como el uso de equipo necesario de medicion. Despues se analizan las posibles medidas de ahorro que se pueden encontrar y las que pueden ser viables.

  16. Methodology for the energy analysis of compressed air systems; Metodologia para analisis energetico de sistemas de aire comprimido

    Energy Technology Data Exchange (ETDEWEB)

    Ramirez, T.; Ambriz, J. J.; Romero, H. [Universidad Autonoma Metropolitana-Iztapalapa, Mexico, D. F. (Mexico)

    1992-12-31

    In this paper a methodology for the diagnosis of compressed air systems, for the identification of the potential energy saving is presented. The methodology consists in detecting the largest number of possible ways of energy saving. For this purpose it is divided into three parts: 1. Compressed air generation. 2. Compressed air distribution. 3. Compressed air users. For each one of the parts, the type of information required to perform the diagnosis study, as well as the necessary measuring equipment needed, is indicated. Afterwards, the possible saving ways that can be found and the ones that can be feasible, are analyzed. [Espanol] En este trabajo se presenta una metodologia para diagnosticar sistemas de aire comprimido, para identificar los potenciales de ahorro de energia. La metodologia consiste en detectar el mayor numero de posibles medidas de ahorro, para lo cual se divide en tres partes: 1. Generacion de aire comprimido. 2. Distribucion del aire comprimido. 3 Usuarios de aire comprimido. Para cada una de las partes se indica el tipo de informacion requerida para realizar el estudio de diagnostico, asi como el uso de equipo necesario de medicion. Despues se analizan las posibles medidas de ahorro que se pueden encontrar y las que pueden ser viables.

  17. Mapping In Vivo Tumor Oxygenation within Viable Tumor by 19F-MRI and Multispectral Analysis

    Directory of Open Access Journals (Sweden)

    Yunzhou Shi

    2013-11-01

    Full Text Available Quantifying oxygenation in viable tumor remains a major obstacle toward a better understanding of the tumor microenvironment and improving treatment strategies. Current techniques are often complicated by tumor heterogeneity. Herein, a novel in vivo approach that combines 19F magnetic resonance imaging (19F-MRIR1 mapping with diffusionbased multispectral (MS analysis is introduced. This approach restricts the partial pressure of oxygen (pO2 measurements to viable tumor, the tissue of therapeutic interest. The technique exhibited sufficient sensitivity to detect a breathing gas challenge in a xenograft tumor model, and the hypoxic region measured by MS 19F-MRI was strongly correlated with histologic estimates of hypoxia. This approach was then applied to address the effects of antivascular agents on tumor oxygenation, which is a research question that is still under debate. The technique was used to monitor longitudinal pO2 changes in response to an antibody to vascular endothelial growth factor (B20.4.1.1 and a selective dual phosphoinositide 3-kinase/mammalian target of rapamycin inhibitor (GDC-0980. GDC-0980 reduced viable tumor pO2 during a 3-day treatment period, and a significant reduction was also produced by B20.4.1.1. Overall, this method provides an unprecedented view of viable tumor pO2 and contributes to a greater understanding of the effects of antivascular therapies on the tumor's microenvironment.

  18. Viable group A streptococci in macrophages during acute soft tissue infection.

    Directory of Open Access Journals (Sweden)

    Pontus Thulin

    2006-03-01

    Full Text Available Group A streptococcal severe soft tissue infections, such as necrotizing fasciitis, are rapidly progressive infections associated with high mortality. Group A streptococcus is typically considered an extracellular pathogen, but has been shown to reside intracellularly in host cells.We characterized in vivo interactions between group A streptococci (GAS and cells involved in innate immune responses, using human biopsies (n = 70 collected from 17 patients with soft tissue infections. Immunostaining and in situ image analysis revealed high amounts of bacteria in the biopsies, even in those collected after prolonged antibiotic therapy. Viability of the streptococci was assessed by use of a bacterial viability stain, which demonstrated viable bacteria in 74% of the biopsies. GAS were present both extracellularly and intracellularly within phagocytic cells, primarily within macrophages. Intracellular GAS were predominantly noted in biopsies from newly involved tissue characterized by lower inflammation and bacterial load, whereas purely extracellular GAS or a combination of intra- and extracellular GAS dominated in severely inflamed tissue. The latter tissue was also associated with a significantly increased amount of the cysteine protease streptococcal pyrogenic exotoxin SpeB. In vitro studies confirmed that macrophages serve as reservoirs for viable GAS, and infection with a speB-deletion mutant produced significantly lower frequencies of cells with viable GAS following infection as compared to the wild-type bacteria.This is the first study to demonstrate that GAS survive intracellularly in macrophages during acute invasive infections. This intracellular presence may have evolved as a mechanism to avoid antibiotic eradication, which may explain our finding that high bacterial load is present even in tissue collected after prolonged intravenous antibiotic therapy. This new insight into the pathogenesis of streptococcal soft tissue infections

  19. Viable Group A Streptococci in Macrophages during Acute Soft Tissue Infection.

    Directory of Open Access Journals (Sweden)

    2006-01-01

    Full Text Available BACKGROUND: Group A streptococcal severe soft tissue infections, such as necrotizing fasciitis, are rapidly progressive infections associated with high mortality. Group A streptococcus is typically considered an extracellular pathogen, but has been shown to reside intracellularly in host cells. METHODS AND FINDINGS: We characterized in vivo interactions between group A streptococci (GAS and cells involved in innate immune responses, using human biopsies (n = 70 collected from 17 patients with soft tissue infections. Immunostaining and in situ image analysis revealed high amounts of bacteria in the biopsies, even in those collected after prolonged antibiotic therapy. Viability of the streptococci was assessed by use of a bacterial viability stain, which demonstrated viable bacteria in 74% of the biopsies. GAS were present both extracellularly and intracellularly within phagocytic cells, primarily within macrophages. Intracellular GAS were predominantly noted in biopsies from newly involved tissue characterized by lower inflammation and bacterial load, whereas purely extracellular GAS or a combination of intra- and extracellular GAS dominated in severely inflamed tissue. The latter tissue was also associated with a significantly increased amount of the cysteine protease streptococcal pyrogenic exotoxin SpeB. In vitro studies confirmed that macrophages serve as reservoirs for viable GAS, and infection with a speB-deletion mutant produced significantly lower frequencies of cells with viable GAS following infection as compared to the wild-type bacteria. CONCLUSIONS: This is the first study to demonstrate that GAS survive intracellularly in macrophages during acute invasive infections. This intracellular presence may have evolved as a mechanism to avoid antibiotic eradication, which may explain our finding that high bacterial load is present even in tissue collected after prolonged intravenous antibiotic therapy. This new insight into the pathogenesis

  20. Para-Hermitian and para-quaternionic manifolds

    International Nuclear Information System (INIS)

    Ivanov, S.; Zamkovoy, S.

    2003-10-01

    A set of canonical para-Hermitian connections on an almost para-Hermitian manifold is defined. A Para-hermitian version of the Apostolov-Gauduchon generalization of the Goldberg-Sachs theorem in General Relativity is given. It is proved that the Nijenhuis tensor of a Nearly para-Kaehler manifolds is parallel with respect to the canonical connection. Salamon's twistor construction on quaternionic manifold is adapted to the para-quaternionic case. A locally conformally hyper-para-Kaehler (hypersymplectic) flat structure with parallel Lee form on the Kodaira-Thurston complex surfaces modeled on S 1 x SL (2, R)-tilde is constructed. Anti-self-dual locally conformally hyper-para-Kaehler (hypersymplectic) neutral metrics with non vanishing Weyl tensor are obtained on the Inoe surfaces. An example of anti-self-dual neutral metric which is not locally conformally hyper-para-Kaehler (hypersymplectic) is constructed. (author)

  1. Para-Hermitian and para-quaternionic manifolds

    Energy Technology Data Exchange (ETDEWEB)

    Ivanov, S [University of Sofia ' St. Kl. Ohridski' , Faculty of Mathematics and Informatics, Sofia (Bulgaria) and Abdus Salam International Centre for Theoretical Physics, Trieste (Italy); Zamkovoy, S [University of Sofia ' St. Kl. Ohridski' , Faculty of Mathematics and Informatics, Sofia (Bulgaria)

    2003-10-01

    A set of canonical para-Hermitian connections on an almost para-Hermitian manifold is defined. A Para-hermitian version of the Apostolov-Gauduchon generalization of the Goldberg-Sachs theorem in General Relativity is given. It is proved that the Nijenhuis tensor of a Nearly para-Kaehler manifolds is parallel with respect to the canonical connection. Salamon's twistor construction on quaternionic manifold is adapted to the para-quaternionic case. A locally conformally hyper-para-Kaehler (hypersymplectic) flat structure with parallel Lee form on the Kodaira-Thurston complex surfaces modeled on S{sup 1} x SL (2, R)-tilde is constructed. Anti-self-dual locally conformally hyper-para-Kaehler (hypersymplectic) neutral metrics with non vanishing Weyl tensor are obtained on the Inoe surfaces. An example of anti-self-dual neutral metric which is not locally conformally hyper-para-Kaehler (hypersymplectic) is constructed. (author)

  2. Advanced Analysis to Distinguish between Physical Decrease and Inactivation of Viable Phages in Aerosol by Quantitating Phage-Specific Particles.

    Science.gov (United States)

    Shimasaki, Noriko; Nojima, Yasuhiro; Sakakibara, Masaya; Kikuno, Ritsuko; Iizuka, Chiori; Okaue, Akira; Okuda, Shunji; Shinohara, Katsuaki

    2018-01-01

     Recent studies have investigated the efficacy of air-cleaning products against pathogens in the air. A standard method to evaluate the reduction in airborne viruses caused by an air cleaner has been established using a safe bacteriophage instead of pathogenic viruses; the reduction in airborne viruses is determined by counting the number of viable airborne phages by culture, after operating the air cleaner. The reduction in the number of viable airborne phages could be because of "physical decrease" or "inactivation". Therefore, to understand the mechanism of reduction correctly, an analysis is required to distinguish between physical decrease and inactivation. The purpose of this study was to design an analysis to distinguish between the physical decrease and inactivation of viable phi-X174 phages in aerosols. We established a suitable polymerase chain reaction (PCR) system by selecting an appropriate primer-probe set for PCR and validating the sensitivity, linearity, and specificity of the primer-probe set to robustly quantify phi-X174-specific airborne particles. Using this quantitative PCR system and culture assay, we performed a behavior analysis of the phage aerosol in a small chamber (1 m 3 ) at different levels of humidity, as humidity is known to affect the number of viable airborne phages. The results revealed that the reduction in the number of viable airborne phages was caused not only by physical decrease but also by inactivation under particular levels of humidity. Our study could provide an advanced analysis to differentiate between the physical decrease and inactivation of viable airborne phages.

  3. Reduced infectivity of waterborne viable but nonculturable Helicobacter pylori strain SS1 in mice.

    Science.gov (United States)

    Boehnke, Kevin F; Eaton, Kathryn A; Fontaine, Clinton; Brewster, Rebecca; Wu, Jianfeng; Eisenberg, Joseph N S; Valdivieso, Manuel; Baker, Laurence H; Xi, Chuanwu

    2017-08-01

    Helicobacter pylori infection has been consistently associated with lack of access to clean water and proper sanitation, but no studies have demonstrated that the transmission of viable but nonculturable (VBNC) H. pylori can occur from drinking contaminated water. In this study, we used a laboratory mouse model to test whether waterborne VBNCH. pylori could cause gastric infection. We performed five mouse experiments to assess the infectivity of VBNCH. pylori in various exposure scenarios. VBNC viability was examined using Live/Dead staining and Biolog phenotype metabolism arrays. High doses of VBNCH. pylori in water were chosen to test the "worst-case" scenario for different periods of time. One experiment also investigated the infectious capabilities of VBNC SS1 using gavage. Further, immunocompromised mice were exposed to examine infectivity among potentially vulnerable groups. After exposure, mice were euthanized and their stomachs were examined for H. pylori infection using culture and PCR methodology. VBNC cells were membrane intact and retained metabolic activity. Mice exposed to VBNCH. pylori via drinking water and gavage were not infected, despite the various exposure scenarios (immunocompromised, high doses) that might have permitted infection with VBNCH. pylori. The positive controls exposed to viable, culturable H. pylori did become infected. While other studies that have used viable, culturable SS1 via gavage or drinking water exposures to successfully infect mice, in our study, waterborne VBNC SS1 failed to colonize mice under all test conditions. Future studies could examine different H. pylori strains in similar exposure scenarios to compare the relative infectivity of the VBNC vs the viable, culturable state, which would help inform future risk assessments of H. pylori in water. © 2017 The Authors. Helicobacter Published by John Wiley & Sons Ltd.

  4. Valoración del residuo de piedra para preparar concreto para senderos peatonales: caso piedra Barichara

    Directory of Open Access Journals (Sweden)

    María Fernanda Serrano-Guzmán

    2014-07-01

    Full Text Available Los indicadores de desarrollo de un país están asociados a la satisfacción de las necesidades básicas de la población y de otras complementarias, como las relacionadas con la  infraestructura vial, que favorecen la competitividad de las regiones. En algunos países en vía de desarrollo es marcado el bajo porcentaje de vías pavimentadas, con la consecuente repercusión económica que la falta de conectividad genera. Por tal razón, se hace necesario ejecutar proyectos que mejoren el estado de las vías, con alternativas que protejan la capa de afirmado. Las lajas de piedra acomodadas con los criterios constructivos de pavimento articulado son opciones viables en aquellos sectores en donde abunda la roca. Barichara, municipio de Santander (Colombia, es una zona caracterizada por la calidad de la piedra que se extrae de manera manual y que se aprovecha para decoración y como material de revestimiento. Durante el proceso de extracción de la piedra, además de afectar la salud del personal encargado de esta labor, se genera un subproducto que impacta negativamente el paisaje. En este estudio se demuestra que la trituración del residuo del proceso de extracción de la piedra permite generar un material aprovechable en mezclas de concreto para senderos peatonales y como mortero de pega de baldosas en área de bajo tránsito.

  5. Entry of Vibrio harveyi and Vibrio fischeri into the viable but nonculturable state

    Digital Repository Service at National Institute of Oceanography (India)

    Ramaiah, Neelam; Ravel, J.; Straube, W.L.; Hill, R.T.; Colwell, R.R.

    , direct viable counts, actively respiring cell counts, nucleoid-containing cell counts, and total counts were determined. V. harveyi incubated at 22 degrees C nutrient-limited artificial seawater (ASW) became nonculturable after approximately 62 and 45 d...

  6. Improved identification of viable myocardium using second harmonic imaging during dobutamine stress echocardiography

    NARCIS (Netherlands)

    F. Sozzi (Fabiola); D. Poldermans (Don); J.J. Bax (Jeroen); A. Elhendy (Abdou); E.C. Vourvouri (Eleni); R. Valkema (Roelf); J. de Sutter; A.F.L. Schinkel (Arend); A. Borghetti; J.R.T.C. Roelandt (Jos)

    2001-01-01

    textabstractOBJECTIVE: To determine whether, compared with fundamental imaging, second harmonic imaging can improve the accuracy of dobutamine stress echocardiography for identifying viable myocardium, using nuclear imaging as a reference. PATIENTS: 30 patients with chronic left

  7. Changes in total viable count and TVB-N content in marinated chicken breast fillets during storage

    Science.gov (United States)

    Baltić, T.; Ćirić, J.; Velebit, B.; Petronijević, R.; Lakićević, B.; Đorđević, V.; Janković, V.

    2017-09-01

    Marination is a popular technique for enhancing meat properties. Depending on the marinade type and ingredients added, marination can improve sensory, chemical and microbiological quality of meat products. In this study, the total viable count and total volatile basic nitrogen (TVB-N) content in marinated chicken breast fillets were investigated. The possible correlation between bacterial growth and formation of TVB-N was also tested. Chicken breast fillets were immersed in a solution of table salt (as a control) orthree different marinades,which consisted of table salt, sodium tripolyphosphate and/or sodium citrate, and stored in air for nine days at 4±1°C. Analyses of the total viable count and TVB-N were performed on days0, 3, 6 and 9 day of storage. The total viable count gradually increased in all examined groups, and statistically significant differences (pchicken were significantly higher (pchicken marinated with sodium citrate was established (pchicken marinated with sodium tripolyphosphate.

  8. de Canadá: una alternativa para México

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Tello

    2006-01-01

    Full Text Available La posibilidad de aplicar telecomunicaciones al trabajo en casa se ve como una de las alternativas viables para disminuir los problemas de congestionamiento vehicular, desperdicio de energía y contaminación del aire en las grandes ciudades. La extensión de las telecomunicaciones a casas y oficinas periféricas, así como la creciente disponibilidad de computadoras, impresoras, fax, etc., han impulsado la exploración de los esquemas telelaborales. Estos esquemas incorporan las propuestas de dicho trabajo en casa, la descentralización del lugar de producción mismo por medio de la creación de oficinas satélite, así como la adopción de nuevas estructuras que permitan reorganizar en una institución las actividades de gerencia y personal. La intención de este artículo es investigar el comportamiento de los viajes cuando los empleados de gobierno se desplazan a sus respectivos lugares de trabajo, con el afán de determinar su disposición a telelaborar en el área metropolitana de la ciudad de Ottawa, en la provincia de Ontario, Canadá. Para lograrlo se diseñó una metodología particular que practica cuatro estudios de campo: dos para examinar el comportamiento de los viajes mencionado en la gerencia y en el personal del departamento de planificación y dos más para la gerencia y el personal del departamento de transporte, ambos del gobierno municipal de Ottawa. Como se esperaba, el estudio reveló que la disposición para telelaborar, cuantificada en términos de apoyo y aceptación por parte de gerentes y empleados respectivamente es significativa: 50% de los gerentes de planificación que participaron en las encuestas y aproximadamente 86% de sus homólogos de transporte apoyaron los arreglos de tipo telelaboral. Asimismo, 41% del personal de planificación aceptó telelaborar en promedio 3.5 días por semana (entre 3 y 4 días y aproximadamente 40% del personal de transporte aceptó hacer lo mismo dos días por semana.

  9. Natural environmental water sources in endemic regions of northeastern Brazil are potential reservoirs of viable Mycobacterium leprae.

    Science.gov (United States)

    Arraes, Maria Luisa Bezerra de Macedo; Holanda, Maísa Viana de; Lima, Luana Nepomuceno Gondim Costa; Sabadia, José Antônio Beltrão; Duarte, Cynthia Romariz; Almeida, Rosa Livia Freitas; Kendall, Carl; Kerr, Ligia Regina Sansigolo; Frota, Cristiane Cunha

    2017-12-01

    The detection of live Mycobacterium leprae in soil and animals other than humans suggests that the environment plays a role in the transmission of leprosy. The objective of this study was to investigate the presence of viable M. leprae in natural water sources used by the local population in five municipalities in the state of Ceará, northeastern Brazil. Samples were collected from 30 different sources. Viable bacilli were identified by reverse transcriptase polymerase chain reaction (PCR) of the M. leprae gyrA gene and sequencing of the PCR products. Physicochemical properties of each water source were also assessed. M. leprae gyrA mRNA was found in 23 (76.7%) of the water sources. No association was found between depth of the water and sample positivity, nor was there any association between the type of water used by the population and sample positivity. An association between viable M. leprae and temperature and pH was found. Georeferencing showed a relation between the residences of leprosy cases and water source containing the bacterium. The finding of viable M. leprae in natural water sources associated with human contact suggests that the environment plays an important role in maintaining endemic leprosy in the study region.

  10. The use of flow cytometry to accurately ascertain total and viable counts of Lactobacillus rhamnosus in chocolate.

    Science.gov (United States)

    Raymond, Yves; Champagne, Claude P

    2015-04-01

    The goals of this study were to evaluate the precision and accuracy of flow cytometry (FC) methodologies in the evaluation of populations of probiotic bacteria (Lactobacillus rhamnosus R0011) in two commercial dried forms, and ascertain the challenges in enumerating them in a chocolate matrix. FC analyses of total (FC(T)) and viable (FC(V)) counts in liquid or dried cultures were almost two times more precise (reproducible) than traditional direct microscopic counts (DCM) or colony forming units (CFU). With FC, it was possible to ascertain low levels of dead cells (FC(D)) in fresh cultures, which is not possible with traditional CFU and DMC methodologies. There was no interference of chocolate solids on FC counts of probiotics when inoculation was above 10(7) bacteria per g. Addition of probiotics in chocolate at 40 °C resulted in a 37% loss in viable cells. Blending of the probiotic powder into chocolate was not uniform which raised a concern that the precision of viable counts could suffer. FCT data can serve to identify the correct inoculation level of a sample, and viable counts (FCV or CFU) can subsequently be better interpreted. Crown Copyright © 2014. Published by Elsevier Ltd. All rights reserved.

  11. Delayed uptake and washout of contrast in non-viable infarcted myocardium shown with dynamic computed tomography

    DEFF Research Database (Denmark)

    Udholm, Sebastian; Laugesen, Sofie; Agger, Peter

    2014-01-01

    BACKGROUND: Assessment of ischemic but potentially viable myocardium plays an important role in the planning of coronary revascularization. Until now SPECT, PET, and MRI have been used to identify viable myocardium. Computed tomography (CT) is increasingly used to diagnose coronary atherosclerosis...... descending artery, followed by removal of the balloon and reperfusion. Four pigs died due to refractory ventricular fibrillation. After 6 weeks, dynamic cardiac CT was performed assessing both wall motion and contrast attenuation. Measurements of attenuation values in Hounsfield units (HU) in the infarct...

  12. Senior health clinics: are they financially viable?

    Science.gov (United States)

    McAtee, Robin E; Crandall, Debra; Wright, Larry D; Beverly, Claudia J

    2009-07-01

    Are hospital-based outpatient interdisciplinary clinics a financially viable alternative for caring for our burgeoning population of older adults in America? Although highly popular, with high patient satisfaction rates among older adults and their families, senior health clinics (SHCs) can be expensive to operate, with limited quantifiable health outcomes. This study analyzed three geriatric hospital-based interdisciplinary clinics in rural Arkansas by examining their patient profiles, revenues, and expenses. It closely examined the effects of the downstream revenue using the multiplier effect and acknowledged other factors that weigh heavily on the success of SHCs and the care of older adults. The findings highlight the similarities and differences in the three clinics' operating and financial structures in addition to the clinics' and providers' productivity. The analysis presents an evidence-based illustration that SHCs can break even or lose large amounts of money.

  13. Software para mejorar la aplicación de técnicas cuantitativas en estudios prospectivos

    Directory of Open Access Journals (Sweden)

    Amaury Cabarcas Álvarez

    2013-06-01

    Full Text Available Durante las dos últimas décadas se ha observado preocupación de organizaciones para lograr competitividad y obtener estabilidad en el mercado, impulsándolas a analizar ventajas de ir a un futuro deseado, haciendo uso de herramientas como los estudios prospectivos. Con el fin de optimizar la aplicación de estudios prospectivos, se han desarrollado herramientas de apoyo que aún no abarcan ciertos intereses de los involucrados como el uso de recursos económicos, ambientales, tecnológicos y humanos. Para suplir la necesidad encontrada, se planteó un software que contribuya al acompañamiento de estudios prospectivos apoyándose de tecnologías Web 2.0 independientemente de técnicas utilizadas por la persona guía del mismo, para lograr racionalizar recursos aprovechando las ventajas que ofrece la web. La investigación permitió concluir que los estudios prospectivos constituyen una alternativa viable para las organizaciones que desean planificar para alcanzar sus objetivos empresariales. Sin embargo, el intento de lograr el futuro deseable traería una serie de costos, que se incrementarán cuando la aplicación de estos estudios se lleve a cabo al margen de las herramientas, software y modelos adecuados. Por esta razón, el hombre debe emplear herramientas tecnológicas que combinen métodos, software, capacidad colaborativa y de integración como la ofrecida por la Web 2.0 para optimizar sus procesos y permitir así, que áreas como la prospectiva, alcancen un alto nivel de eficiencia y masificación

  14. Mathematical modelling of the viable epidermis: impact of cell shape and vertical arrangement

    KAUST Repository

    Wittum, Rebecca; Naegel, Arne; Heisig, Michael; Wittum, Gabriel

    2017-01-01

    In-silico methods are valuable tools for understanding the barrier function of the skin. The key benefit is that mathematical modelling allows the interplay between cell shape and function to be elucidated. This study focuses on the viable (living

  15. Economically viable biochemical processes for the advanced rural biorefinery and downstream recovery operations

    Science.gov (United States)

    Rural biorefineries offer an alternative to traditional ethanol production by providing the opportunity to produce fuel on site to reduce costs associated with biomass transportation thus making the fuel economically viable. Widespread installation of rural biorefineries could lead to increased upt...

  16. The installation IGCC power plans in the petroleum refinement: international experiences and lessons for Mexico; La instalacion de plantas IGCC en la refinacion de petroleo: experiencias internacionales y lecciones para Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez, Joel [Ecole du Petrole et des Moteurs, Institut Franzais du Petrole, (France)

    2004-06-15

    refinacion de petroleo ha crecido en un 14.3 % a nivel mundial a partir de 1996, en donde ya se contaba con una capacidad instalada de 160 MW. A finales de 2003 se alcanzo una capacidad instalada de 2,500 MW. El crecimiento en la instalacion de las plantas IGCC se presenta primordialmente en Europa, siendo Italia y Espana paises lideres en la construccion de esta tecnologia en la refinacion de petroleo. No obstante, paises como Holanda, Japon, Singapur y Estados Unidos cuentan con plantas IGCC para la generacion de electricidad e hidrogeno, las cuales aprovechan combustibles de bajo valor como residuos de vacio, coque de petroleo, asfalto, combustibles liquidos, entre otros. En Mexico, la instalacion de plantas IGCC en la refinacion de petroleo es nula, sin embargo Petroleos Mexicanos cuenta con la aprobacion del gobierno para la instalacion de plantas de cogeneracion en sus instalaciones. Esta aprobacion permitiria a PEMEX llevar a cabo proyectos para la instalacion de plantas IGCC, especificamente en PEMEX Refinacion, para la generacion de electricidad e hidrogeno a partir del aprovechamiento de residuos pesados de bajo valor economico. La oportunidad que ofrece la instalacion de plantas IGCC en la refinacion de petroleo es dirigida hacia la comercializacion de la electricidad e hidrogeno, lo que impulsaria a PEMEX Refinacion entrar en la competencia del mercado electrico en Mexico.

  17. Immunization against chlamydial genital infection in guinea pigs with UV-inactivated and viable chlamydiae administered by different routes

    International Nuclear Information System (INIS)

    Rank, R.G.; Batteiger, B.E.; Soderberg, L.S.

    1990-01-01

    Female guinea pigs were immunized with viable or UV light-inactivated chlamydiae, belonging to the species Chlamydia psittaci, by intravenous, subcutaneous, oral, or ocular routes. All animals were then inoculated vaginally with viable chlamydiae to determine the extent of protection against challenge infection induced by the various regimens. The course of genital infection was significantly reduced in intensity in all groups of animals except the unimmunized controls and those animals immunized orally with inactivated antigen. Guinea pigs immunized with viable antigen were more likely to develop resistance to challenge infection and, in general, had a significantly greater degree of protection than animals immunized with inactivated antigen. No one route seemed superior in producing a protective response. Animals in all groups demonstrating protection developed serum and secretion immunoglobulin G antibody responses to chlamydiae. Lymphocyte proliferative reactions to chlamydial antigen were variable among groups. Immunoblot analysis of serum and secretions indicated a wide range of antibody specificities, but most protected animals produced antibodies to the major outer membrane protein, lipopolysaccharide, and the 61-kilodalton protein. No definitive associations could be made between the increased ability of immunization with viable organisms to produce resistance to challenge infection and a particular immune parameter. These data indicate that viable chlamydiae given by various routes are able to induce a strong immune response which can provide resistance against reinfection in some cases or at least reduce the degree of infection to a greater degree than inactivated antigen. However, complete resistance to genital tract infection may be difficult to obtain and alternate immunizations strategies may have to be developed

  18. Diseño y cálculo de un dispositivo para la obtención de agua potable por condensación de la humedad del aire

    OpenAIRE

    Rodríguez Pajarón, Pablo

    2016-01-01

    Este trabajo de fin de grado tiene como objetivo el diseño y el cálculo de un ciclo de compresión mecánica simple, para su utilización en un dispositivo capaz de obtener agua potable a partir de la humedad del aire. Ante la situación de escasez de agua apta para el consumo humano que sufren muchas zonas del planeta, o los problemas de abastecimiento puntuales a raíz de catástrofes naturales, esta opción es una tecnología viable y cada vez más desarrollada. Sin embargo, el inconveniente al ...

  19. In search of viable business models for development: sustainable energy in developing countries

    NARCIS (Netherlands)

    Kolk, A.; van den Buuse, D.

    2012-01-01

    Purpose - Although the crucial role of business, and of business-based approaches, in development is increasingly emphasised by academics and practitioners, insight is lacking into the "whether and how" of viable business models, in environmental, social and economical terms. This article analyses

  20. Enterocolic lymphocytic phlebitis: statistical analysis of histology features in viable and ischemic bowel.

    Science.gov (United States)

    Medlicott, Shaun A C; Guggisberg, Kelly A; DesCôteaux, Jean-Gaston; Beck, Paul

    2006-07-01

    Enterocolic lymphocytic phlebitis is a rare cause of segmental ischemic enterocolitis. This artery-sparing transmural vasculitis is classically a circumferential phlebitis with perivenular lymphocyte cuffing and thrombi in the absence of systemic manifestations. Myointimal hyperplasia may represent a chronic phase of enterocolic lymphocytic phlebitis. Subclinical or early stage enterocolic lymphocytic phlebitis is not well delineated. We analyzed 600 submucosal and subserosal veins from both ischemic and intact bowel segments to discern if vascular morphology varied between sites. Crescentic and circumferential lymphocytic phlebitis is more common in viable bowel than in the ischemic segment. A nonsignificant trend was found for increased crescentic morphology between intact bowel remote from the ischemic focus compared with that adjacent to the ischemic focus. Hallmarks of ischemic bowel are necrotizing phlebitis and thrombi formation. Thrombophlebitis morphology is distinctly different in viable and ischemic bowel, changing from the classic lymphocytic to necrotizing lesions respectively.

  1. Blood flow, flow reserve, and glucose utilization in viable and nonviable myocardium in patients with ischemic cardiomyopathy.

    Science.gov (United States)

    Zhang, Xiaoli; Schindler, Thomas H; Prior, John O; Sayre, James; Dahlbom, Magnus; Huang, Sung-Cheng; Schelbert, Heinrich R

    2013-04-01

    The aim of the study was to determine whether glucose uptake in viable myocardium of ischemic cardiomyopathy patients depends on rest myocardial blood flow (MBF) and the residual myocardial flow reserve (MFR). Thirty-six patients with ischemic cardiomyopathy (left ventricular ejection fraction 25 ± 10 %) were studied with (13)N-ammonia and (18)F-fluorodeoxyglucose (FDG) positron emission tomography (PET). Twenty age-matched normals served as controls. Regional MBF was determined at rest and during dipyridamole hyperemia and regional FDG extraction was estimated from regional FDG to (13)N-ammonia activity ratios. Rest MBF was reduced in viable (0.42 ± 0.18 ml/min per g) and nonviable regions (0.32 ± 0.09 ml/min per g) relative to remote regions (0.68 ± 0.23 ml/min per g, p MFRs did not differ significantly (p > 0.05). Compared to MFR in remote myocardium, MFRs in viable regions were similar (1.39 ± 0.56 vs 1.70 ± 0.45, p > 0.05) but were significantly lower in nonviable regions (1.23 ± 0.43, p MFRs (r =-0.424, p MFRs in viable myocardium are associated with increasing glucose extraction that likely reflects a metabolic adaptation of remodeling hibernating myocytes.

  2. Irradiación subletal de fibroblastos murinos 3T3 con rayos X para su utilización como capa celular alimentadora

    Directory of Open Access Journals (Sweden)

    Andrea Ramírez-Téllez

    2015-05-01

    Full Text Available Los queratinocitos, las principales células de la epidermis, requieren una capa alimentadora de células viables mitóticamente inactivadas para su supervivencia y proliferación in vitro. Para la producción de capas alimentadoras a partir de fibroblastos murinos 3T3, se evaluaron cuatro dosis de radiación con rayos X. Los efectos de las dosis se evaluaron mediante recuentos celulares y determinación de la viabilidad (MTT, adhesión (eficiencia de plateo y migración celular (scratch wound healing. Además, se encontraron evidencias que sugieren el arresto mitótico mediante una tinción nuclear (GIEMSA. Se observó que una dosis de radiación correspondiente a 80 Gy generó daños suficientes en la línea celular para causar el arresto mitótico, manteniendo a la vez la actividad metabólica basal durante 25 días.

  3. Finding viable models in SUSY parameter spaces with signal specific discovery potential

    Science.gov (United States)

    Burgess, Thomas; Lindroos, Jan Øye; Lipniacka, Anna; Sandaker, Heidi

    2013-08-01

    Recent results from ATLAS giving a Higgs mass of 125.5 GeV, further constrain already highly constrained supersymmetric models such as pMSSM or CMSSM/mSUGRA. As a consequence, finding potentially discoverable and non-excluded regions of model parameter space is becoming increasingly difficult. Several groups have invested large effort in studying the consequences of Higgs mass bounds, upper limits on rare B-meson decays, and limits on relic dark matter density on constrained models, aiming at predicting superpartner masses, and establishing likelihood of SUSY models compared to that of the Standard Model vis-á-vis experimental data. In this paper a framework for efficient search for discoverable, non-excluded regions of different SUSY spaces giving specific experimental signature of interest is presented. The method employs an improved Markov Chain Monte Carlo (MCMC) scheme exploiting an iteratively updated likelihood function to guide search for viable models. Existing experimental and theoretical bounds as well as the LHC discovery potential are taken into account. This includes recent bounds on relic dark matter density, the Higgs sector and rare B-mesons decays. A clustering algorithm is applied to classify selected models according to expected phenomenology enabling automated choice of experimental benchmarks and regions to be used for optimizing searches. The aim is to provide experimentalist with a viable tool helping to target experimental signatures to search for, once a class of models of interest is established. As an example a search for viable CMSSM models with τ-lepton signatures observable with the 2012 LHC data set is presented. In the search 105209 unique models were probed. From these, ten reference benchmark points covering different ranges of phenomenological observables at the LHC were selected.

  4. Droplet digital PCR improves absolute quantification of viable lactic acid bacteria in faecal samples.

    Science.gov (United States)

    Gobert, Guillaume; Cotillard, Aurélie; Fourmestraux, Candice; Pruvost, Laurence; Miguet, Jean; Boyer, Mickaël

    2018-03-14

    Analysing correlations between the observed health effects of ingested probiotics and their survival in digestive tract allows adapting their preparations for food. Tracking ingested probiotic in faecal samples requires accurate and specific tools to quantify live vs dead cells at strain level. Traditional culture-based methods are simpler to use but they do not allow quantifying viable but non-cultivable (VBNC) cells and they are poorly discriminant below the species level. We have set up a viable PCR (vPCR) assay combining propidium monoazide (PMA) treatment and either real time quantitative PCR (qPCR) or droplet digital PCR (ddPCR) to quantify a Lactobacillus rhamnosus and two Lactobacillus paracasei subsp. paracasei strains in piglet faeces. Adjustments of the PMA treatment conditions and reduction of the faecal sample size were necessary to obtain accurate discrimination between dead and live cells. The study also revealed differences of PMA efficiency among the two L. paracasei strains. Both PCR methods were able to specifically quantify each strain and provided comparable total bacterial counts. However, quantification of lower numbers of viable cells was best achieved with ddPCR, which was characterized by a reduced lower limit of quantification (improvement of up to 1.76 log 10 compared to qPCR). All three strains were able to survive in the piglets' gut with viability losses between 0.78 and 1.59 log 10 /g faeces. This study shows the applicability of PMA-ddPCR to specific quantification of small numbers of viable bacterial cells in the presence of an important background of unwanted microorganisms, and without the need to set up standard curves. It also illustrates the need to adapt PMA protocols according to the final matrix and target strain, even for closely related strains. The PMA-ddPCR approach provides a new tool to quantify bacterial survival in faecal samples from a preclinical and clinical trial. Copyright © 2018 The Authors. Published by

  5. Acupuntura un tratamiento viable para las adicciones en Colombia

    OpenAIRE

    Hernán López-Suescún; Miguel Cote-Menéndez; Héctor Rojas-Ramírez

    2013-01-01

    Resumen La acupuntura es una antigua técnica terapéutica desarrollada en China, que ha evidenciado ser efectiva en síntomas como las náuseas, vómito y dolor dentario. A pesar del sustento fisiológico que posibilitaría un uso efectivo en otras patologías, incluyendo el campo de las adicciones, los estudios son contradictorios, posiblemente por la diferencias de visión entre la medicina oriental y la occidental. El consumo de psicoactivos es un problema de salud pública en Colombia y en el mund...

  6. Real-time monitoring of non-viable airborne particles correlates with airborne colonies and represents an acceptable surrogate for daily assessment of cell-processing cleanroom performance.

    Science.gov (United States)

    Raval, Jay S; Koch, Eileen; Donnenberg, Albert D

    2012-10-01

    Airborne particulate monitoring is mandated as a component of good manufacturing practice. We present a procedure developed to monitor and interpret airborne particulates in an International Organization for Standardization (ISO) class 7 cleanroom used for the cell processing of Section 351 and Section 361 products. We collected paired viable and non-viable airborne particle data over a period of 1 year in locations chosen to provide a range of air quality. We used receiver operator characteristic (ROC) analysis to determine empirically the relationship between non-viable and viable airborne particle counts. Viable and non-viable particles were well-correlated (r(2) = 0.78), with outlier observations at the low end of the scale (non-viable particles without detectable airborne colonies). ROC analysis predicted viable counts ≥ 0.5/feet(3) (a limit set by the United States Pharmacopeia) at an action limit of ≥ 32 000 particles (≥ 0.5 µ)/feet(3), with 95.6% sensitivity and 50% specificity. This limit was exceeded 2.6 times during 18 months of retrospective daily cleanroom data (an expected false alarm rate of 1.3 times/year). After implementing this action limit, we were alerted in real time to an air-handling failure undetected by our hospital facilities management. A rational action limit for non-viable particles was determined based on the correlation with airborne colonies. Reaching or exceeding the action limit of 32 000 non-viable particles/feet(3) triggers suspension of cleanroom cell-processing activities, deep cleaning, investigation of air handling, and a deviation management process. Our full procedure for particle monitoring is available as an online supplement.

  7. The ghost of extinction: Preservation values and minimum viable population in wildlife models

    NARCIS (Netherlands)

    Eiswerth, M.E.; Kooten, van G.C.

    2009-01-01

    The inclusion of a minimum viable population in bioeconomic modeling creates at least two complications that are not resolved by using a modified logistic growth function. The first complication can be dealt with by choosing a different depensational growth function. The second complication relates

  8. Viable Bacteria Associated with Red Blood Cells and Plasma in Freshly Drawn Blood Donations

    DEFF Research Database (Denmark)

    Damgaard, Christian; Magnussen, Karin; Enevold, Christian

    2015-01-01

    ) or blue lactose plates. For identification colony PCR was performed using primers targeting 16S rDNA. SETTING: Blood donors attending Capital Region Blood Bank, Copenhagen University Hospital, Rigshospitalet, Hvidovre, Denmark, October 29th to December 10th 2013. PARTICIPANTS: 60 donors (≥50 years old....... CONCLUSIONS: Viable bacteria are present in blood from donors self-reported as medically healthy, indicating that conventional test systems employed by blood banks insufficiently detect bacteria in plasma. Further investigation is needed to determine whether routine testing for anaerobic bacteria and testing......OBJECTIVES: Infection remains a leading cause of post-transfusion mortality and morbidity. Bacterial contamination is, however, detected in less than 0.1% of blood units tested. The aim of the study was to identify viable bacteria in standard blood-pack units, with particular focus on bacteria from...

  9. Defined Combinations of Cryomedia and Thawing Extenders Influence the Viable X-Y Boar Sperm Ratio in Vitro.

    Science.gov (United States)

    Korchunjit, W; Kaeoket, K; Kitiyanant, Y; Taylor, J; Wongtawan, T

    It is believed that plasma membrane X- and Y-chromosome bearing sperm are different; therefore the freezing and thawing process may affect X- and Y-sperm differently. The objective of this study was to investigate the effect of cryomedia and thawing extenders on the survival of X and Y-sperm. Three different cryomedia and thawing extenders were compared. Viable motile sperm were separated using a swim-up technique. Real-time PCR was used to identify the sperm type. Using CryoA for freezing and Beltsville-Thawing-Solution (BTS) as the thawing extender yielded significantly higher numbers of viable motile Y sperm (64 percent) than control (48 percent) (P semen freezing with CryoC and thawing with Androstar Plus gave a significantly lower number of viable motile Y sperm (32 percent) than control (51 percent). Our results revealed that defined combinations of cryomedia and thawing extenders significantly altered the survival ratio of frozen-thawed X-Y sperm in vitro, which has potential implications for artificial insemination.

  10. Cultivo de microalgas Isochrysis galbana y Nannochloropsis sp. para alimentación de larvas de peces marinos

    Directory of Open Access Journals (Sweden)

    María Fernanda Pereira Gutiérrez

    2017-09-01

    Full Text Available En la producción de alimento vivo, representado principalmente por microalgas, se buscan nuevas técnicas de medios de cultivo sustituyendo las fórmulas tradicionales en aras de reducir costos. Teniendo en cuenta esto, se usó un medio no convencional (fertilizantes industriales para producir las microalgas Isochrysis galbana y Nannochloropsis sp. en cultivos estáticos, y se alcanzaron densidades de 7,5 × 106 cel mL–1 de Nannochloropsis sp. en 1000 L y 0,265 × 106 cel mL–1 de I. galbana en 250 L. Hubo un crecimiento exponencial, que se alcanzó entre los tres y los ocho días, en que se duplicaron las densidades iniciales. Se sugiere cosechar en lapsos cortos (hasta cuatro días, debido a la proliferación de contaminantes y la disminución de la calidad de las células con el tiempo. Se concluye que el medio diseñado para el enriquecimiento del cultivo es una alternativa viable para la producción de las microalgas que permite satisfacer la demanda alimenticia en el levante de larvas de peces marinos.

  11. Una Medicion de la Pobreza: Un Enfoque Complementario para el Caso Colombiano

    Directory of Open Access Journals (Sweden)

    Sinisterra Paz Gullermo A.

    2003-08-01

    Full Text Available

    Sinisterra Paz, Guillermo A. " Una Medicion de la Pobreza: Un Enfoque Complementario para el Caso Colombiano" , Cuadernos de Economia, V. XXII , n. 38, Bogotá, 2003, paginas 127 - 150.

    En la literatura sobre la pobreza es cada vez mas aceptada la perspectiva sociologica de privaciones relativas, entendida como los los sentimientos de frustacion que tienen las personas al interior de la sociedad, al no tener acceso a las oportunidades que si son viables para otros. A partir de esto se han estructurado varios modelos que buscan medir dicha definicion de pobreza desde el punto de vista de la teoria economica. Se busca dar una vision complementaria a la forma de calcular la pobreza en Colombia, ultilizando el concepto de privaciones relativas, que es mucho mas rico en informacion que los conceptos normalmente utilizados, esto permite diagnosticar mejor quienes son pobres y quienes no lo son. Ademas, son aproximacimaciones que han sido tomadas por lo general en los paises desarrollados. Se utilizo una aproximacion econometrica a la definicion de la pobreza basada en tecnicas de analisis de secciones  transversales.

  12. Establishment of a Viable Population of Red-Cockaded Woodpeckers at the Savannah River Site

    International Nuclear Information System (INIS)

    Johnston, P.A.

    2002-01-01

    Report on program's objective to restore viable population of Red-cockaded woodpecker at SRS. Several management strategies were used to promote population expansion of Red-cockaded woodpecker and reduction of interspecific competition with Red-Cockaded woodpecker

  13. On the trace-free Einstein equations as a viable alternative to general relativity

    International Nuclear Information System (INIS)

    Ellis, George F R; Van Elst, Henk; Murugan, Jeff; Uzan, Jean-Philippe

    2011-01-01

    The quantum field theoretical prediction for the vacuum energy density leads to a value for the effective cosmological constant that is incorrect by between 60 and 120 orders of magnitude. We review an old proposal of replacing Einstein's field equations by their trace-free part (the trace-free Einstein equations), together with an independent assumption of energy-momentum conservation by matter fields. While this does not solve the fundamental issue of why the cosmological constant has the value that is observed cosmologically, it is indeed a viable theory that resolves the problem of the discrepancy between the vacuum energy density and the observed value of the cosmological constant. However, one has to check that, as well as preserving the standard cosmological equations, this does not destroy other predictions, such as the junction conditions that underlie the use of standard stellar models. We confirm that no problems arise here: hence, the trace-free Einstein equations are indeed viable for cosmological and astrophysical applications. (papers)

  14. Waste association in mass for coating formulations: a viable alternative to dispose; Associacao de residuos em formulacoes de massas para revestimentos: uma alternativa viavel ao descarte

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, E.A.C.; Soares Filho, J.E.; Souza, F.J.P.; Almeida, V.S. de; Oliveira, T.M. de, E-mail: erikcferreira@gmail.com [Universidade Federal do Rio Grande do Norte (UFRN), Natal RN (Brazil)

    2016-07-01

    The ceramic coatings industries are able to use in their formulations whose waste Eco disposal make the costly disposal, being able to reduce production costs by replacing traditional inputs for mining and industrial waste. Their raw materials are classified as plasticizers, fluxes and structural according to their physicochemical characteristics. Since waste falls within these classifications, their use in formulations becomes a viable and attractive alternative from an ecological point of view and marketing. Several studies have attested to waste incorporating viability porcelains formulations, however, is not common to find studies evaluating the addition of more than one simultaneously in formulations. It is the objective of the study, to examine whether fine waste rock and kaolin together with traditional raw materials are able to produce porcelain wet as technological properties defined by the NBR-13818. (author)

  15. Cultivos de cobertura,¿una alternativa viable para la región semirárida pampeana? Cover crops: a viable alternative for the semiarid Pampa region?

    Directory of Open Access Journals (Sweden)

    Romina Fernández

    2012-12-01

    N disponible en el suelo para el cultivo de maíz y sorgo. El rendimiento de maíz fue dependiente de la disponibilidad hídrica en el suelo a la siembra, ya que en B fue de 1015 kg ha-1, mientras que el rendimiento promedio con antecesor CC fue de 4044 kg ha-1. Para el caso del cultivo de sorgo, el manejo previo (con o sin CC no condicionó los rendimientos del mismo, debido a su ciclo fenológico más largo que facilitó que el cultivo aprovechara mayores precipitaciones específicamente durante el período entre siembra y floración.The effect of cover crops (CC on the provision of soil cover, N sequestration, accumulation of available water and the yield of a subsequent summer crop was evaluated. A field experiment with the following treatments was established: fallow without cover crop (B and rye as CC, control (CT, and fertilized (CF in a randomized complete block design with plots divided in two moments of drying of CC: July (CTJ and CFJ respectively and August (CTA and CFA respectively. On all treatments, two summer crops (CV, corn and sorghum were planted after fallowing at the end of November. Soil moisture and nitrate-N were determined at seeding of the CC, during their growing season, and at planting and flowering of corn and sorghum. Consumptive water use (UC and water use efficiency (EUA of CC, corn and sorghum were calculated. The standing biomass of CC was determined at the two dates of drying (J and A, and at planting of the summer crops and during their growing period the CC biomass litter was measured. The carbon (C, nitrogen (N and phosphorus (P contents of the dry matter were determined for all sampling dates. At planting of the summer crops, B treatment had less available water stored in the soil than CC, and J stored more water than A treatment. Total dry matter production of CC was higher in F and when dried in A. The CC sequestered important amounts of C, N and P, preventing potential leaching losses of N during the fallow, and CC litter

  16. Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

    OpenAIRE

    Zhao, Xihong; Zhong, Junliang; Wei, Caijiao; Lin, Chii-Wann; Ding, Tian

    2017-01-01

    The viable but non-culturable (VBNC) state, a unique state in which a number of bacteria respond to adverse circumstances, was first discovered in 1982. Unfortunately, it has been reported that many foodborne pathogens can be induced to enter the VBNC state by the limiting environmental conditions during food processing and preservation, such as extreme temperatures, drying, irradiation, pulsed electric field, and high pressure stress, as well as the addition of preservatives and disinfectant...

  17. Rapid assessment of viable but non-culturable Bacillus coagulans MTCC 5856 in commercial formulations using Flow cytometry

    Science.gov (United States)

    Majeed, Muhammed; Majeed, Shaheen; Nagabhushanam, Kalyanam; Punnapuzha, Ardra; Philip, Sheena

    2018-01-01

    Accurate enumeration of bacterial count in probiotic formulation is imperative to ensure that the product adheres to regulatory standards and citation in consumer product label. Standard methods like plate count, can enumerate only replicating bacterial population under selected culture conditions. Viable but non culturable bacteria (VBNC) retain characteristics of living cells and can regain cultivability by a process known as resuscitation. This is a protective mechanism adapted by bacteria to evade stressful environmental conditions. B. coagulans MTCC 5856(LactoSpore®) is a probiotic endospore which can survive for decades in hostile environments without dividing. In the present study, we explored the use of flow cytometry to enumerate the viable count of B. coagulans MTCC 5856 under acidic and alkaline conditions, high temperature and in commercial formulations like compressed tablets and capsules. Flow cytometry (FCM) was comparable to plate count method when the spores were counted at physiological conditions. We show that VBNC state is induced in B. coagulans MTCC 5856by high temperature and acidic pH. The cells get resuscitated under physiological conditions and FCM was sensitive to detect the VBNC spores. Flow cytometry showed excellent ability to assess the viable spore count in commercial probiotic formulations of B. coagulans MTCC 5856. The results establish Flow cytometry as a reliable method to count viable bacteria in commercial probiotic preparations. Sporulation as well as existence as VBNC could contribute to the extreme stability of B. coagulans MTCC 5856. PMID:29474436

  18. Rapid assessment of viable but non-culturable Bacillus coagulans MTCC 5856 in commercial formulations using Flow cytometry.

    Directory of Open Access Journals (Sweden)

    Muhammed Majeed

    Full Text Available Accurate enumeration of bacterial count in probiotic formulation is imperative to ensure that the product adheres to regulatory standards and citation in consumer product label. Standard methods like plate count, can enumerate only replicating bacterial population under selected culture conditions. Viable but non culturable bacteria (VBNC retain characteristics of living cells and can regain cultivability by a process known as resuscitation. This is a protective mechanism adapted by bacteria to evade stressful environmental conditions. B. coagulans MTCC 5856(LactoSpore® is a probiotic endospore which can survive for decades in hostile environments without dividing. In the present study, we explored the use of flow cytometry to enumerate the viable count of B. coagulans MTCC 5856 under acidic and alkaline conditions, high temperature and in commercial formulations like compressed tablets and capsules. Flow cytometry (FCM was comparable to plate count method when the spores were counted at physiological conditions. We show that VBNC state is induced in B. coagulans MTCC 5856by high temperature and acidic pH. The cells get resuscitated under physiological conditions and FCM was sensitive to detect the VBNC spores. Flow cytometry showed excellent ability to assess the viable spore count in commercial probiotic formulations of B. coagulans MTCC 5856. The results establish Flow cytometry as a reliable method to count viable bacteria in commercial probiotic preparations. Sporulation as well as existence as VBNC could contribute to the extreme stability of B. coagulans MTCC 5856.

  19. Rapid assessment of viable but non-culturable Bacillus coagulans MTCC 5856 in commercial formulations using Flow cytometry.

    Science.gov (United States)

    Majeed, Muhammed; Majeed, Shaheen; Nagabhushanam, Kalyanam; Punnapuzha, Ardra; Philip, Sheena; Mundkur, Lakshmi

    2018-01-01

    Accurate enumeration of bacterial count in probiotic formulation is imperative to ensure that the product adheres to regulatory standards and citation in consumer product label. Standard methods like plate count, can enumerate only replicating bacterial population under selected culture conditions. Viable but non culturable bacteria (VBNC) retain characteristics of living cells and can regain cultivability by a process known as resuscitation. This is a protective mechanism adapted by bacteria to evade stressful environmental conditions. B. coagulans MTCC 5856(LactoSpore®) is a probiotic endospore which can survive for decades in hostile environments without dividing. In the present study, we explored the use of flow cytometry to enumerate the viable count of B. coagulans MTCC 5856 under acidic and alkaline conditions, high temperature and in commercial formulations like compressed tablets and capsules. Flow cytometry (FCM) was comparable to plate count method when the spores were counted at physiological conditions. We show that VBNC state is induced in B. coagulans MTCC 5856by high temperature and acidic pH. The cells get resuscitated under physiological conditions and FCM was sensitive to detect the VBNC spores. Flow cytometry showed excellent ability to assess the viable spore count in commercial probiotic formulations of B. coagulans MTCC 5856. The results establish Flow cytometry as a reliable method to count viable bacteria in commercial probiotic preparations. Sporulation as well as existence as VBNC could contribute to the extreme stability of B. coagulans MTCC 5856.

  20. Immediate natural tooth pontic: A viable yet temporary prosthetic solution: A patient reported outcome

    Directory of Open Access Journals (Sweden)

    Sudhir Bhandari

    2012-01-01

    Conclusion: The concept of immediate pontic placement is surely a viable treatment option and promises an excellent transient esthetic solution for a lost tooth as well as enables good preparation of the extraction site for future prosthetic replacement.

  1. USO DE SUBPRODUCTOS DE CAÑA PANELERA COMO SUPLEMENTO ALIMENTICIO PARA GANADO BOVINO Y PARA EVITAR LA CONTAMINACIÓN AMBIENTAL

    Directory of Open Access Journals (Sweden)

    Martínez N.

    2005-12-01

    Full Text Available El presente artículo se enmarca dentro de la caracterización del sistema de producción bovina del municipio de Utica (Cundinamarca y dentro de los problemas más importantes que lo afectan. Como resultado, el estudio identificó la escasez de alimento de buena calidad en épocas secas como la principal limitante del productor bovino del municipio. Aprovechando una ventaja presente en la zona, como es la elaboración de panela (proceso a través del cual resultan algunos subproductos, como bagazo y melote, que pueden ser utilizados como alternativa alimenticia para los bovinos en cualquier época del año, se planteó un experimento en el que se elaboraron bloques multinutricionales con base en dichos subproductos. El experimento se adelantó en una finca de un productor y se utilizaron 57 novillos cebús con edades entre los 11 y los 24 meses y pesos entre los 124 y los 463 kilogramos. Los animales se dividieron dos grupos que se mantuvieron en pasturas no manejadas. Al gru¬po experimental se le ofrecieron adicionalmente bloques multinutricionales. Los animales fueron examinados para comprobar su estado fisiológico, con el fin de determinar posibles inconvenientes sanitarios que pudieran afectar el ensayo. Se realizaron pesajes para medir las ganancias de peso en los dos grupos. Los resultados mostraron promedios de ganancias diarias de peso similares a Pr < 5%, donde fueron ligeramente superiores las del grupo alimentado con los bloques multinutricionales (0,848 vs. 0,769 kg animal/día. Los aportes más importantes del experimento fueron, primero, brindar una opción para que el productor pueda alimentar a sus animales en épocas de escasez y evitar que tenga que venderlos a bajos precios. El segundo aporte fue abrir la posibilidad de usar los productos de la elaboración de la panela, que en muchas ocasiones se convierten en desechos que no son usados y que contaminan las fuentes de agua, de una forma económicamente viable.

  2. Metodología para un proceso apreciativo, dinámico y colaborativo: III Plan de Salud de Canarias

    Directory of Open Access Journals (Sweden)

    José Joaquín O'Shanahan Juan

    2016-11-01

    Full Text Available La necesidad de nuevos enfoques de planificación estratégica con la incorporación de la visión de profesionales y ciudadanos ha orientado un nuevo modelo para el III Plan de Salud de Canarias (IIIPSC. Se propone un proceso participativo con soporte de metodología cualitativa en dos etapas: 1 fase local: estudio cuantitativo-cualitativo a través de una acción formativa y de investigación-acción-participación, y 2 fase insular: conferencias de salud, con debate sobre resultados en sesiones presenciales en cada área de salud (isla y propuestas de acción. El proceso define una priorización de problemas y un plan de acción específico para cada isla mediante operaciones consideradas viables, agrupadas por temas y ponderadas según el potencial impacto sobre los problemas priorizados. Este proceso de interacción puede contribuir a orientar los cambios de modelo de planificación y la toma de decisiones en política sanitaria, y se encuentra recogido en el Proyecto del IIIPSC para su tramitación parlamentaria.

  3. Generating power stations and optimization energetic of processes; Centrales generadoras y optimacion energetica de procesos

    Energy Technology Data Exchange (ETDEWEB)

    Gutierrez Ramirez, Ranulfo; Fernandez Montiel, Manuel Francisco [Instituto de Investigaciones Electricas, Temixco, Morelos (Mexico)

    1999-07-01

    Some recent experiences of the Management of Thermal Processes of the Instituto de Investigaciones Electricas (IIE) related to the works on generating power stations of electricity, plants of cogeneration and energy saving are presented. [Spanish] Se presentan algunas experiencias recientes de la Gerencia de Procesos Termicos del Instituto de Investigaciones Electricas (IIE) relacionadas con los trabajos sobre centrales generadoras de electricidad, plantas de cogeneracion y ahorro de energia.

  4. Estudio de factibilidad para la producción de aceite vegetal comestible de aguacate producido en la finca "Cuatro Hermanos" de la provincia de Manabí y propuesta de plan de exportación dirigido al mercado de Londres, Reino Unido.

    OpenAIRE

    Gómez Zambrano, Karem Lissette

    2015-01-01

    Se realizó un estudio de factibilidad para exportar aceite de aguacate a Londres, Reino Unido producido en la Finca “Cuatro Hermanos” de la provincia de Manabí. Comprobando que la finca cuenta con la capacidad productiva para satisfacer la demanda de 250 toneladas. La tasa interna de retorno fue 29.93%, demostrando que el proyecto es viable. A feasibility study was made to export edible vegetable oil avocado to London, United of Kingdom produced in the farm "Cuatro hermanos” in the provinc...

  5. Modelling the number of viable vegetative cells of Bacillus cereus passing through the stomach

    NARCIS (Netherlands)

    Wijnands, L.M.; Pielaat, A.; Dufrenne, J.B.; Zwietering, M.H.; Leusden, van F.M.

    2009-01-01

    Aims: Model the number of viable vegetative cells of B. cereus surviving the gastric passage after experiments in simulated gastric conditions. Materials and Methods: The inactivation of stationary and exponential phase vegetative cells of twelve different strains of Bacillus cereus, both mesophilic

  6. Viable Intrauterine Pregnancy and Coexisting Molar Pregnancy in a Bicornuate Uterus: A Rare Presentation

    Directory of Open Access Journals (Sweden)

    Kavitha Krishnamoorthy

    2016-01-01

    Full Text Available A complete hydatidiform mole with a viable coexisting fetus (CMCF is a rare occurrence. Similarly, Mullerian anomalies such as a bicornuate uterus are uncommon variants of normal anatomy. We report a case of a 40-year-old female with a known bicornuate uterus presenting at 13 weeks gestation with vaginal bleeding. Ultrasound findings showed a healthy viable pregnancy in the right horn with complete molar pregnancy in the left horn. After extensive counseling, the patient desired conservative management, however, was unable to continue due to profuse vaginal bleeding. The patient underwent suction dilation and curettage under general anesthesia and evacuation of the uterine horns. Postoperatively, the patient was followed until serum beta-human chorionic gonadotropin (β-hCG level dropped to <5 mU. This is the first case of a CMCF reported in a bicornuate uterus, diagnosed with the use of ultrasound imaging.

  7. Mobilization of Viable Tumor Cells Into the Circulation During Radiation Therapy

    International Nuclear Information System (INIS)

    Martin, Olga A.; Anderson, Robin L.; Russell, Prudence A.; Ashley Cox, R.; Ivashkevich, Alesia; Swierczak, Agnieszka; Doherty, Judy P.; Jacobs, Daphne H.M.; Smith, Jai; Siva, Shankar; Daly, Patricia E.; Ball, David L.

    2014-01-01

    Purpose: To determine whether radiation therapy (RT) could mobilize viable tumor cells into the circulation of non-small cell lung cancer (NSCLC) patients. Methods and Materials: We enumerated circulating tumor cells (CTCs) by fluorescence microscopy of blood samples immunostained with conventional CTC markers. We measured their DNA damage levels using γ-H2AX, a biomarker for radiation-induced DNA double-strand breaks, either by fluorescence-activated cell sorting or by immunofluorescence microscopy. Results: Twenty-seven RT-treated NSCLC patients had blood samples analyzed by 1 or more methods. We identified increased CTC numbers after commencement of RT in 7 of 9 patients treated with palliative RT, and in 4 of 8 patients treated with curative-intent RT. Circulating tumor cells were also identified, singly and in clumps in large numbers, during RT by cytopathologic examination (in all 5 cases studied). Elevated γ-H2AX signal in post-RT blood samples signified the presence of CTCs derived from irradiated tumors. Blood taken after the commencement of RT contained tumor cells that proliferated extensively in vitro (in all 6 cases studied). Circulating tumor cells formed γ-H2AX foci in response to ex vivo irradiation, providing further evidence of their viability. Conclusions: Our findings provide a rationale for the development of strategies to reduce the concentration of viable CTCs by modulating RT fractionation or by coadministering systemic therapies

  8. Mobilization of Viable Tumor Cells Into the Circulation During Radiation Therapy

    Energy Technology Data Exchange (ETDEWEB)

    Martin, Olga A. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Molecular Radiation Biology Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); The Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, VIC (Australia); Anderson, Robin L. [The Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, VIC (Australia); Metastasis Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Russell, Prudence A. [Department of Anatomical Pathology, St. Vincent Hospital, Fitzroy, VIC (Australia); Ashley Cox, R. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Ivashkevich, Alesia [Molecular Radiation Biology Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Laboratory of DNA Repair and Genomics, Centre for Innate Immunity and Infectious Disease, Monash Institute for Medical Research, Monash University, Clayton, VIC (Australia); Swierczak, Agnieszka; Doherty, Judy P. [Metastasis Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Jacobs, Daphne H.M. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Smith, Jai [Molecular Radiation Biology Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Siva, Shankar; Daly, Patricia E. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); Ball, David L. [Division of Radiation Oncology and Cancer Imaging, Peter MacCallum Cancer Centre, East Melbourne, VIC (Australia); The Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, VIC (Australia); and others

    2014-02-01

    Purpose: To determine whether radiation therapy (RT) could mobilize viable tumor cells into the circulation of non-small cell lung cancer (NSCLC) patients. Methods and Materials: We enumerated circulating tumor cells (CTCs) by fluorescence microscopy of blood samples immunostained with conventional CTC markers. We measured their DNA damage levels using γ-H2AX, a biomarker for radiation-induced DNA double-strand breaks, either by fluorescence-activated cell sorting or by immunofluorescence microscopy. Results: Twenty-seven RT-treated NSCLC patients had blood samples analyzed by 1 or more methods. We identified increased CTC numbers after commencement of RT in 7 of 9 patients treated with palliative RT, and in 4 of 8 patients treated with curative-intent RT. Circulating tumor cells were also identified, singly and in clumps in large numbers, during RT by cytopathologic examination (in all 5 cases studied). Elevated γ-H2AX signal in post-RT blood samples signified the presence of CTCs derived from irradiated tumors. Blood taken after the commencement of RT contained tumor cells that proliferated extensively in vitro (in all 6 cases studied). Circulating tumor cells formed γ-H2AX foci in response to ex vivo irradiation, providing further evidence of their viability. Conclusions: Our findings provide a rationale for the development of strategies to reduce the concentration of viable CTCs by modulating RT fractionation or by coadministering systemic therapies.

  9. A viable logarithmic f(R) model for inflation

    Energy Technology Data Exchange (ETDEWEB)

    Amin, M.; Khalil, S. [Center for Fundamental Physics, Zewail City of Science and Technology,6 October City, Giza (Egypt); Salah, M. [Center for Fundamental Physics, Zewail City of Science and Technology,6 October City, Giza (Egypt); Department of Mathematics, Faculty of Science, Cairo University,Giza (Egypt)

    2016-08-18

    Inflation in the framework of f(R) modified gravity is revisited. We study the conditions that f(R) should satisfy in order to lead to a viable inflationary model in the original form and in the Einstein frame. Based on these criteria we propose a new logarithmic model as a potential candidate for f(R) theories aiming to describe inflation consistent with observations from Planck satellite (2015). The model predicts scalar spectral index 0.9615

  10. Bio-electrosprayed multicellular zebrafish embryos are viable and develop normally

    International Nuclear Information System (INIS)

    Clarke, Jonathan D W; Jayasinghe, Suwan N

    2008-01-01

    Bio-electrosprays are rapidly emerging as a viable protocol for directly engineering living cells. This communication reports the bio-electrospraying of multicellular organisms, namely zebrafish embryos. The results demonstrate that the bio-electrospray protocol fails to induce any embryological perturbations. In addition to analysing overall embryo morphology, we use transgenic embryos that express green fluorescent protein in specific brain neurons to determine that neuronal numbers and organization are completely normal. These results demonstrate that the bio-electrospraying protocol does not interfere with the complex gene regulation and cell movements required for the development of a multicellular organism. (communication)

  11. Acortando la brecha digital para la trazabilidad sanitaria: el problema de la transferencia tecnológica en la ganadería sonorense, caso SITAGAN

    Directory of Open Access Journals (Sweden)

    Gloria Ciria Valdez-Gardea

    2011-01-01

    Full Text Available Se reporta un esfuerzo por desa rrollar metodologías para el diseño y transferencia de nuevas tecnologías para el sector ganadero en Sonora. Se identificó la necesidad de construir canales de comunicación efectiva, permanente, viables económica y culturalmente, con el productor como premisa ineludible para el establecimiento de modelos exitosos de transferencia tecnológica. Se muestran datos que apuntan al uso del Internet como la tecnología con mayor potencial histórico para establecer este puente de comunicación. Se presentan resultados de treinta entrevistas de profundidad, semiestructuradas y un par de encuestas realizadas entre 500 ganaderos sonorenses en relación al nacimiento, promoción y adopción del Sistema de Trazabilidad y Administración Ganadera (SITAGAN. Las características (funcionalidad y lenguaje de SITAGAN se definieron a partir de las necesidades y preocupaciones expresadas por los ganaderos en las entrevistas y la primera encuesta. La segunda encuesta permitió identificar canales para promover y educar a los ganaderos en torno al uso del Internet y el software. Se desarrollaron dos versiones de SITAGAN para operar con o sin acceso a Internet, ambas uentan con video tutoriales y ayudas en cada tema, así como generadores de reportes y análisis de productividad.

  12. Preliminary stochastic model for managing Vibrio parahaemolyticus and total viable bacterial counts in a Pacific oyster (Crassostrea gigas) supply chain.

    Science.gov (United States)

    Fernandez-Piquer, Judith; Bowman, John P; Ross, Tom; Estrada-Flores, Silvia; Tamplin, Mark L

    2013-07-01

    Vibrio parahaemolyticus can accumulate and grow in oysters stored without refrigeration, representing a potential food safety risk. High temperatures during oyster storage can lead to an increase in total viable bacteria counts, decreasing product shelf life. Therefore, a predictive tool that allows the estimation of both V. parahaemolyticus populations and total viable bacteria counts in parallel is needed. A stochastic model was developed to quantitatively assess the populations of V. parahaemolyticus and total viable bacteria in Pacific oysters for six different supply chain scenarios. The stochastic model encompassed operations from oyster farms through consumers and was built using risk analysis software. Probabilistic distributions and predictions for the percentage of Pacific oysters containing V. parahaemolyticus and high levels of viable bacteria at the point of consumption were generated for each simulated scenario. This tool can provide valuable information about V. parahaemolyticus exposure and potential control measures and can help oyster companies and regulatory agencies evaluate the impact of product quality and safety during cold chain management. If coupled with suitable monitoring systems, such models could enable preemptive action to be taken to counteract unfavorable supply chain conditions.

  13. Deletion of ultraconserved elements yields viable mice

    Energy Technology Data Exchange (ETDEWEB)

    Ahituv, Nadav; Zhu, Yiwen; Visel, Axel; Holt, Amy; Afzal, Veena; Pennacchio, Len A.; Rubin, Edward M.

    2007-07-15

    Ultraconserved elements have been suggested to retainextended perfect sequence identity between the human, mouse, and ratgenomes due to essential functional properties. To investigate thenecessities of these elements in vivo, we removed four non-codingultraconserved elements (ranging in length from 222 to 731 base pairs)from the mouse genome. To maximize the likelihood of observing aphenotype, we chose to delete elements that function as enhancers in amouse transgenic assay and that are near genes that exhibit markedphenotypes both when completely inactivated in the mouse as well as whentheir expression is altered due to other genomic modifications.Remarkably, all four resulting lines of mice lacking these ultraconservedelements were viable and fertile, and failed to reveal any criticalabnormalities when assayed for a variety of phenotypes including growth,longevity, pathology and metabolism. In addition more targeted screens,informed by the abnormalities observed in mice where genes in proximityto the investigated elements had been altered, also failed to revealnotable abnormalities. These results, while not inclusive of all thepossible phenotypic impact of the deleted sequences, indicate thatextreme sequence constraint does not necessarily reflect crucialfunctions required for viability.

  14. Building Viable and Sustainable Regional Netchains: Case Studies of Regional Pork Netchains in Spain, Germany, and The Netherlands

    Directory of Open Access Journals (Sweden)

    Rannia Nijhoff‐Savvaki

    2012-06-01

    Full Text Available The present paper aims to give insight into critical issues contributing to the successful building of viable and sustainable regional netchains, focussing on chain governance mechanisms (coordination mechanisms, quality management systems, information systems and social embedding. It uses three representative case studies from the regional pork sector in Spain, Germany, and The Netherlands, illustrating different trajectories to commercially viable and sustainable regional netchains. By analysing the cases on each critical aspect, the present paper describes important issues and proposes specific elements of further research, including on the roles for(nongovernmental organisations.

  15. Cummins L10G in Kenworth truck 'viable today'

    International Nuclear Information System (INIS)

    Anon.

    1996-01-01

    As the culmination of five years of developmental work by Cummins Engines, BC Research Inc., and BC Gas Utility Ltd., a T800 Kenworth truck was outfitted with a new Cummins L10G natural gas engine, and two lightweight fully -wrapped Dynetek cylinders; it was pronounced to be 'a viable clean truck today'. The L10G spark-ignited engine operates at a relatively high peak efficiency of 37 per cent and is commercially available to meet the current California Air Resources Board heavy duty vehicle emission standards without the use of a catalytic converter. The L10G engine produces no particulate emissions, a very significant advantage, in view of the fact that particulate emissions have been identified as major contributors to respiratory ailments

  16. Biocatalytically active silCoat-composites entrapping viable Escherichia coli.

    Science.gov (United States)

    Findeisen, A; Thum, O; Ansorge-Schumacher, M B

    2014-02-01

    Application of whole cells in industrial processes requires high catalytic activity, manageability, and viability under technical conditions, which can in principle be accomplished by appropriate immobilization. Here, we report the identification of carrier material allowing exceptionally efficient adsorptive binding of Escherichia coli whole cells hosting catalytically active carbonyl reductase from Candida parapsilosis (CPCR2). With the immobilizates, composite formation with both hydrophobic and hydrophilized silicone was achieved, yielding advanced silCoat-material and HYsilCoat-material, respectively. HYsilCoat-whole cells were viable preparations with a cell loading up to 400 mg(E. coli) · g(-1)(carrier) and considerably lower leaching than native immobilizates. SilCoat-whole cells performed particularly well in neat substrate exhibiting distinctly increased catalytic activity.

  17. Articular Cartilage Repair Using Marrow Stimulation Augmented with a Viable Chondral Allograft: 9-Month Postoperative Histological Evaluation

    Directory of Open Access Journals (Sweden)

    James K. Hoffman

    2015-01-01

    Full Text Available Marrow stimulation is frequently employed to treat focal chondral defects of the knee. However, marrow stimulation typically results in fibrocartilage repair tissue rather than healthy hyaline cartilage, which, over time, predisposes the repair to failure. Recently, a cryopreserved viable chondral allograft was developed to augment marrow stimulation. The chondral allograft is comprised of native viable chondrocytes, chondrogenic growth factors, and extracellular matrix proteins within the superficial, transitional, and radial zones of hyaline cartilage. Therefore, host mesenchymal stem cells that infiltrate the graft from the underlying bone marrow following marrow stimulation are provided with the optimal microenvironment to undergo chondrogenesis. The present report describes treatment of a trochlear defect with marrow stimulation augmented with this novel chondral allograft, along with nine month postoperative histological results. At nine months, the patient demonstrated complete resolution of pain and improvement in function, and the repair tissue consisted of 85% hyaline cartilage. For comparison, a biopsy obtained from a patient 8.2 months after treatment with marrow stimulation alone contained only 5% hyaline cartilage. These outcomes suggest that augmenting marrow stimulation with the viable chondral allograft can eliminate pain and improve outcomes, compared with marrow stimulation alone.

  18. Plan de marketing estratégico para promocionar y comercializar la pitahaya producida por la compañía "San Vicente S.A." en la ciudad de Guayaquil.

    OpenAIRE

    Apráez Orellana, Dennise Alexandra; Proaño Martínez, Ivet Gabriela

    2015-01-01

    La presente investigación pretende crear un plan de marketing estratégico para promocionar y comercializar la Pitahaya en los supermercados y así mismo comprobar que este proyecto es factible basado en los resultados de rentabilidad del mismo. The current investigation pretends to create a strategic marketing plan to promote and commercialize the Pitahaya in the supermarkets and also prove that this project is viable based in the results of profitability.

  19. Identificación de mejores prácticas de calidad para el desarrollo de software para ser implementadas en los procesos de formación de la media técnica en programación - Alianza Futuro Digital Medellín

    OpenAIRE

    Raquejo Álvarez, Claudia Marcela

    2015-01-01

    Este proyecto centró la atención en la identificación de buenas prácticas de calidad para el desarrollo de software, centradas en el proceso y no en el producto de software, viables de ser incorporadas en la formación de los estudiantes de la media técnica en informática y programación en instituciones de educación básica y media pertenecientes a la Alianza Futuro Digital Medellín; esto con el fin de aportar elementos significativos en la formación técnica, que se verán reflejados en la calid...

  20. 9 CFR 113.27 - Detection of extraneous viable bacteria and fungi in live vaccines.

    Science.gov (United States)

    2010-01-01

    ... bacteria and fungi in live vaccines. 113.27 Section 113.27 Animals and Animal Products ANIMAL AND PLANT... bacteria and fungi in live vaccines. Unless otherwise specified by the Administrator or elsewhere exempted... Seed Bacteria shall be tested for extraneous viable bacteria and fungi as prescribed in this section. A...

  1. Estimation of Viable Biomass In Wastewater And Activated Sludge By Determination of ATP, Oxygen Utilization Rate And FDA Hydrolysis

    DEFF Research Database (Denmark)

    Jørgensen, Poul-Erik; Eriksen, T.; Jensen, B.K.

    1992-01-01

    ATP content, oxygen utilization rate (OUR) and fluorescein diacetate (FDA) hydrolysis were tested for the ability to express the amount of viable biomass in wastewater and activated sludge. The relationship between biomass and these activity parameters was established in growth cultures made...... with biomass, while FDA hydrolysis in the sludge failed to show any such correlation. Conversion factors of 3 mg ATP/g dw, 300 mg O2/h g dw and 0.4 A/h (mg dw/ml) for ATP, OUR and FDA methods, respectively, were calculated. When the methods were applied for in situ determinations in four different wastewater...... plants, it was found that ATP content and respiration rate estimated viable biomass to range from 81 to 293 mg dw/g SS for raw wastewater and from 67 to 187 mg dw/g SS for activated sludge with a rather weak correlation between ATP and respiration measurements. The FDA hydrolysis estimated viable biomass...

  2. Viable-but-Nonculturable Listeria monocytogenes and Salmonella enterica Serovar Thompson Induced by Chlorine Stress Remain Infectious

    Directory of Open Access Journals (Sweden)

    Callum J. Highmore

    2018-04-01

    Full Text Available The microbiological safety of fresh produce is monitored almost exclusively by culture-based detection methods. However, bacterial food-borne pathogens are known to enter a viable-but-nonculturable (VBNC state in response to environmental stresses such as chlorine, which is commonly used for fresh produce decontamination. Here, complete VBNC induction of green fluorescent protein-tagged Listeria monocytogenes and Salmonella enterica serovar Thompson was achieved by exposure to 12 and 3 ppm chlorine, respectively. The pathogens were subjected to chlorine washing following incubation on spinach leaves. Culture data revealed that total viable L. monocytogenes and Salmonella Thompson populations became VBNC by 50 and 100 ppm chlorine, respectively, while enumeration by direct viable counting found that chlorine caused a <1-log reduction in viability. The pathogenicity of chlorine-induced VBNC L. monocytogenes and Salmonella Thompson was assessed by using Caenorhabditis elegans. Ingestion of VBNC pathogens by C. elegans resulted in a significant life span reduction (P = 0.0064 and P < 0.0001, and no significant difference between the life span reductions caused by the VBNC and culturable L. monocytogenes treatments was observed. L. monocytogenes was visualized beyond the nematode intestinal lumen, indicating resuscitation and cell invasion. These data emphasize the risk that VBNC food-borne pathogens could pose to public health should they continue to go undetected.

  3. Inactivation of viable Ascaris eggs by reagents during enumeration.

    Science.gov (United States)

    Nelson, K L; Darby, J L

    2001-12-01

    Various reagents commonly used to enumerate viable helminth eggs from wastewater and sludge were evaluated for their potential to inactivate Ascaris eggs under typical laboratory conditions. Two methods were used to enumerate indigenous Ascaris eggs from sludge samples. All steps in the methods were the same except that in method I a phase extraction step with acid-alcohol (35% ethanol in 0.1 N H(2)SO(4)) and diethyl ether was used whereas in method II the extraction step was avoided by pouring the sample through a 38-microm-mesh stainless steel sieve that retained the eggs. The concentration of eggs and their viability were lower in the samples processed by method I than in the samples processed by method II by an average of 48 and 70%, respectively. A second set of experiments was performed using pure solutions of Ascaris suum eggs to elucidate the effect of the individual reagents and relevant combination of reagents on the eggs. The percentages of viable eggs in samples treated with acid-alcohol alone and in combination with diethyl ether or ethyl acetate were 52, 27, and 4%, respectively, whereas in the rest of the samples the viability was about 80%. Neither the acid nor the diethyl ether alone caused any decrease in egg viability. Thus, the observed inactivation was attributed primarily to the 35% ethanol content of the acid-alcohol solution. Inactivation of the eggs was prevented by limiting the direct exposure to the extraction reagents to 30 min and diluting the residual concentration of acid-alcohol in the sample by a factor of 100 before incubation. Also, the viability of the eggs was maintained if the acid-alcohol solution was replaced with an acetoacetic buffer. None of the reagents used for the flotation step of the sample cleaning procedure (ZnSO(4), MgSO(4), and NaCl) or during incubation (0.1 N H(2)SO(4) and 0.5% formalin) inactivated the Ascaris eggs under the conditions studied.

  4. Tiempo de latencia para semen colectado de Colossoma macropomum “Gamitana” en solución sacarosa

    Directory of Open Access Journals (Sweden)

    Ehrlich Llasaca-Calizaya

    2014-12-01

    Full Text Available El objetivo fue estimar el tiempo de latencia (almacenamiento, para el semen de Colossoma macropomum, “gamitana” en solución de 400 mM de Sacarosa. Se consideró aceptable los niveles de motilidad superiores al 40%, lo cual garantiza eficientes tasas de fertilización. Para el desarrollo del experimento se colectó 2 lotes de semen inmótiles de gamitana (inducidos con Conceptal®, los cuales posteriormente fueron activados con agua destilada. El primer lote estuvo constituido por semen en sacarosa 400 mM, puro, a temperatura ambiente y refrigerado (4°C. La motilidad fue evaluada, cada hora, hasta la 7ma hora post colecta. El segundo lote con un semen en sacarosa 400mM a temperatura refrigerada y evaluada cada 12 horas. Los resultados del primer lote de semen demuestran que a partir de la 7ma hora hacia delante los índices de motilidad caen significativamente por debajo del 40%. Los resultados del segundo lote demuestran la viabilidad de utilizar solución de sacarosa, como medio de conservación, para mantener semen refrigerado por 2 días y activarlos con agua destilada. El proceso de extraer y colocar repetidas veces la misma muestra en refrigeración, limita el tiempo de viabilidad de semen con sacarosa en 8 horas aproximadamente. La utilización de sacarosa como medio para almacenar semen inmotil viable de gamitana, ayuda a conservar los espermatozoides por tiempos relativamente cortos.

  5. Tiempo de latencia para semen colectado de Colossoma macropomum “Gamitana” en solución sacarosa

    Directory of Open Access Journals (Sweden)

    Ehrlich Llasaca-Calizaya

    2015-01-01

    Full Text Available El objetivo fue estimar el tiempo de latencia (almacenamiento, para el semen de Colossoma macropomum, “gamitana” en solución de 400 mM de Sacarosa. Se consideró aceptable los niveles de motilidad superiores al 40%, lo cual garantiza eficientes tasas de fertilización. Para el desarrollo del experimento se colectó 2 lotes de semen inmótiles de gamitana (inducidos con Conceptal®, los cuales posteriormente fueron activados con agua destilada. El primer lote estuvo constituido por semen en sacarosa 400 mM, puro, a temperatura ambiente y refrigerado (4°C. La motilidad fue evaluada, cada hora, hasta la 7ma hora post colecta. El segundo lote con un semen en sacarosa 400mM a temperatura refrigerada y evaluada cada 12 horas. Los resultados del primer lote de semen demuestran que a partir de la 7ma hora hacia delante los índices de motilidad caen significativamente por debajo del 40%. Los resultados del segundo lote demuestran la viabilidad de utilizar solución de sacarosa, como medio de conservación, para mantener semen refrigerado por 2 días y activarlos con agua destilada. El proceso de extraer y colocar repetidas veces la misma muestra en refrigeración, limita el tiempo de viabilidad de semen con sacarosa en 8 horas aproximadamente. La utilización de sacarosa como medio para almacenar semen inmotil viable de gamitana, ayuda a conservar los espermatozoides por tiempos relativamente cortos.

  6. Corporate strategy and viable future land use: Planning for closure from the outset of mining

    International Nuclear Information System (INIS)

    Warhurst, A.

    2000-01-01

    This paper provides an overview of the environmental impact of mining on viable future land use and underlines the imperative of improved environmental management and closure planning. It argues that pollution prevention, through planning for closure, can lead to cost-effective strategies for sustainable minerals development and viable future land use. This seems to be most true for greenfield sites since, generally, the earlier closure planning and pollution prevention is built into a project, the more cost-effective and environmentally benign closure will be. Further, for greenfield sites, pollution prevention techniques can be employed from the outset, at the stages of exploration and mine development, and then monitored and improved through the operation stage to closure, and can be kept in place to manage future land use. The paper discusses how global changes in the industry, following the liberalisation of investment regimes, and mergers and strategic alliances between key firms, has, by virtue of the diffusion of new technology, led to further opportunities to prevent pollution and optimise future land use through planning for closure from the outset. The objectives and components of closure plans are also reviewed as the paper draws on case studies to highlight some of the possible constraints and challenges to pollution prevention that may be faced at the level of both public policy and corporate strategy. The article concludes by suggesting a forward-looking approach to integrated environmental management and viable future land-use planning based on a dynamic model for environmental management. (author)

  7. Jurisdicción y medios alternativos de resolución de conflictos: una opción para las cuestiones ambientales

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Lunelli

    2014-10-01

    Full Text Available A medida que las sociedades se fueron tornando más complejas, comenzaron a surgir nuevas categorías de conflictos, como es el caso de los conflictos ambientales. Sin embargo, el sistema procesal vigente todavía se rige por un ordenamiento jurídico positivista, apegado fundamentalmente a las directrices enmarcadas en la ley, ya que en la actualidad se utiliza la misma estructura judicial para resolver todo tipo de apremios. Como resultado de ello, se observa una deficiencia, por parte del Estado, en tutelar los nuevos derechos que surgen en una sociedad en constante evolución. Dado este contexto, el presente trabajo plantea la necesidad de utilizar medios alternativos de resolución de controversias, tales como la mediación-conciliación como opciones viables para poner fin a los conflictos ambientales, y con ello obtener una solución mediante medidas específicas al caso concreto.

  8. Cellular and soluble components decrease the viable pathogen counts in milk from dairy cows with subclinical mastitis.

    Science.gov (United States)

    Koshiishi, Tomoko; Watanabe, Masako; Miyake, Hajime; Hisaeda, Keiichi; Isobe, Naoki

    2017-08-10

    The present study was undertaken to clarify the factors that reduce the viable pathogen count in milk collected from the udders of subclinical mastitic cows during preservation. Milk was centrifuged to divide somatic cells (cellular components, precipitates) and antimicrobial peptides (soluble components, supernatants without fat layer); each fraction was cultured with bacteria, and the number of viable bacteria was assessed prior to and after culture. In 28.8% of milk samples, we noted no viable bacteria immediately after collection; this value increased significantly after a 5-hr incubation of milk with cellular components but not with soluble components (48.1 and 28.8%, respectively). After culture with cellular components, the numbers of bacteria (excluding Staphylococcus aureus and Streptococcus uberis) and yeast decreased dramatically, although the differences were not statistically significant. After cultivation with soluble components, only yeasts showed a tendency toward decreased mean viability, whereas the mean bacterial counts of S. uberis and T. pyogenes tended to increase after 5-hr preservation with soluble components. These results suggest that most pathogens in high somatic cell count (SCC) milk decreased during preservation at 15 to 25°C, due to both the cellular components and antimicrobial components in the milk. Particularly, the cellular components more potently reduced bacterial counts during preservation.

  9. Viable but non-culturable Listeria monocytogenes on parsley leaves and absence of recovery to a culturable state.

    Science.gov (United States)

    Dreux, N; Albagnac, C; Federighi, M; Carlin, F; Morris, C E; Nguyen-the, C

    2007-10-01

    To investigate the presence of viable but non-culturable Listeria monocytogenes during survival on parsley leaves under low relative humidity (RH) and to evaluate the ability of L. monocytogenes to recover from VBNC to culturable state under satured humidity. Under low RH (47-69%) on parsley leaves, the initial number of L. monocytogenes populations counted on non selective media (10(9) L. monocytogenes per leaf on TSA) was reduced by 6 log10 scales in 15 days, whereas number of viable L. monocytogenes counted under the microscope was reduced by 3-4 log10 scales, indicating the presence of VBNC cells. This was demonstrated on three L. monocytogenes strains (EGDe, Bug 1995 and LmP60). Changing from low to 100% RH permitted an increase of the culturable counts of L. monocytogenes and this growth was observed only when residual culturable cells were present. Moreover, VBNC L. monocytogenes inoculated on parsley leaves did not become culturable after incubation under 100% RH. Dry conditions induced VBNC L. monocytogenes on parsley leaves but these VBNC were likely unable to recover culturability after transfer to satured humidity. Enumeration on culture media presumably under-estimates the number of viable L. monocytogenes on fresh produce after exposure to low RH.

  10. Detection and Quantification of Viable and Nonviable Trypanosoma cruzi Parasites by a Propidium Monoazide Real-Time Polymerase Chain Reaction Assay

    Science.gov (United States)

    Cancino-Faure, Beatriz; Fisa, Roser; Alcover, M. Magdalena; Jimenez-Marco, Teresa; Riera, Cristina

    2016-01-01

    Molecular techniques based on real-time polymerase chain reaction (qPCR) allow the detection and quantification of DNA but are unable to distinguish between signals from dead or live cells. Because of the lack of simple techniques to differentiate between viable and nonviable cells, the aim of this study was to optimize and evaluate a straightforward test based on propidium monoazide (PMA) dye action combined with a qPCR assay (PMA-qPCR) for the selective quantification of viable/nonviable epimastigotes of Trypanosoma cruzi. PMA has the ability to penetrate the plasma membrane of dead cells and covalently cross-link to the DNA during exposure to bright visible light, thereby inhibiting PCR amplification. Different concentrations of PMA (50–200 μM) and epimastigotes of the Maracay strain of T. cruzi (1 × 105–10 parasites/mL) were assayed; viable and nonviable parasites were tested and quantified by qPCR with a TaqMan probe specific for T. cruzi. In the PMA-qPCR assay optimized at 100 μM PMA, a significant qPCR signal reduction was observed in the nonviable versus viable epimastigotes treated with PMA, with a mean signal reduction of 2.5 logarithm units and a percentage of signal reduction > 98%, in all concentrations of parasites assayed. This signal reduction was also observed when PMA-qPCR was applied to a mixture of live/dead parasites, which allowed the detection of live cells, except when the concentration of live parasites was low (10 parasites/mL). The PMA-qPCR developed allows differentiation between viable and nonviable epimastigotes of T. cruzi and could thus be a potential method of parasite viability assessment and quantification. PMID:27139452

  11. FACTIBILIDAD PARA IMPLEMENTAR UNA MICROEMPRESA PROCESADORA DE BOTONES Y ARTESANÍAS DE TAGUA EN MEMBRILLO

    Directory of Open Access Journals (Sweden)

    Silvia Annabelle Saltos Flores

    2012-11-01

    Full Text Available El análisis de la factibilidad para la implementación de una microempresa procesadora de botones y artesanías de tagua (Phytelephas macrocarpa es el objetivo principal de este trabajo, para contribuir con el desarrollo socio-económico de la parroquia Membrillo. Las técnicas utilizadas en la investigación fueron el estudio de mercado, el análisis técnico, de impacto ambiental y económico-financiero; se determinó la oferta y demanda de los botones y las artesanías de tagua. Los proveedores de materia prima de la zona en estudio, tienen un precio promedio de 7.20 dólares el quintal. Al introducir el producto al mercado se espera ofrecer 528 gruesas/ mes de botones y 4000 unidades/mes de artesanías de tagua, convirtiéndose este en el mercado meta de este estudio. Con el análisis técnico se pudo determinar la constitución y el tamaño óptimo de la microempresa, para 162 m2 en total. El estudio de impacto ambiental arroja que la aplicación de la matriz de Leopold se determinaron los impactos ambientales negativos de la propuesta, tanto en la construcción de la empresa como su puesta en marcha, siendo viable desde el punto de vista económico y financiero, para ello se consideran los indicadores: el Flujo de Caja, el Valor Actual Neto (VAN, la Tasa Interna de Retorno (TIR y el Período de Retorno de la Inversión (PRI.

  12. Production of viable male unreduced gametes in Brassica interspecific hybrids is genotype specific and stimulated by cold temperatures

    Directory of Open Access Journals (Sweden)

    Cowling Wallace A

    2011-06-01

    Full Text Available Abstract Background Unreduced gametes (gametes with the somatic chromosome number may provide a pathway for evolutionary speciation via allopolyploid formation. We evaluated the effect of genotype and temperature on male unreduced gamete formation in Brassica allotetraploids and their interspecific hybrids. The frequency of unreduced gametes post-meiosis was estimated in sporads from the frequency of dyads or giant tetrads, and in pollen from the frequency of viable giant pollen compared with viable normal pollen. Giant tetrads were twice the volume of normal tetrads, and presumably resulted from pre-meiotic doubling of chromosome number. Giant pollen was defined as pollen with more than 1.5 × normal diameter, under the assumption that the doubling of DNA content in unreduced gametes would approximately double the pollen cell volume. The effect of genotype was assessed in five B. napus, two B. carinata and one B. juncea parents and in 13 interspecific hybrid combinations. The effect of temperature was assessed in a subset of genotypes in hot (day/night 30°C/20°C, warm (25°C/15°C, cool (18°C/13°C and cold (10°C/5°C treatments. Results Based on estimates at the sporad stage, some interspecific hybrid genotypes produced unreduced gametes (range 0.06 to 3.29% at more than an order of magnitude higher frequency than in the parents (range 0.00% to 0.11%. In nine hybrids that produced viable mature pollen, the frequency of viable giant pollen (range 0.2% to 33.5% was much greater than in the parents (range 0.0% to 0.4%. Giant pollen, most likely formed from unreduced gametes, was more viable than normal pollen in hybrids. Two B. napus × B. carinata hybrids produced 9% and 23% unreduced gametes based on post-meiotic sporad observations in the cold temperature treatment, which was more than two orders of magnitude higher than in the parents. Conclusions These results demonstrate that sources of unreduced gametes, required for the triploid

  13. Detection of viable Escherichia coli O157:H7 in ground beef by propidium monoazide real-time PCR.

    Science.gov (United States)

    Liu, Yarui; Mustapha, Azlin

    2014-01-17

    Escherichia coli O157:H7 associated with food has caused many serious public health problems in recent years. However, only viable cells of this pathogen can cause infections, and false-positive detection caused by dead cells can lead to unnecessary product recalls. The objective of this study was to develop and optimize a method that combines propidium monoazide (PMA) staining with real-time PCR to detect only viable cells of E. coli O157:H7 in ground beef. PMA is a DNA intercalating dye that can penetrate compromised membranes of dead cells and bind to cellular DNA, preventing its amplification via a subsequent PCR. Three strains of E. coli O157:H7 (505B, G5310 and C7927) at concentrations of 10(0) to 10(8)CFU/mL were used as live cells. Dead cells were obtained by heating cell suspensions at 85°C for 15 min. Suspensions were treated with PMA and the optimized assay was applied to artificially contaminated ground beef with two different fat contents (10% and 27%). DNA was extracted and amplified by TaqMan® real-time PCR assay targeting the uidA gene for detection of E. coli O157:H7. Plasmid pUC19 was added as an internal amplification control (IAC). A treatment of 25 μM PMA with a 10-min light exposure on ice was sufficient to eliminate DNA from 10(8) dead E. coli O157:H7 cells/mL. The optimized assay could detect as low as 10(2) CFU/mL viable E. coli O157:H7 in pure culture and 10(5) CFU/g in ground beef, in the presence of 10(6)/mL or g of dead cells. With an 8-h enrichment, 1 CFU/g viable E. coli O157:H7 in ground beef was detectable without interference from 10(6) dead cells/g. In conclusion, the PMA real-time PCR could effectively detect viable E. coli O157:H7 without being compromised by dead cells. Copyright © 2013 Elsevier B.V. All rights reserved.

  14. Fake Journals: Their Features and Some Viable Ways to Distinguishing Them

    DEFF Research Database (Denmark)

    Hemmat Esfe, Mohammad; Wongwises, Somchai; Asadi, Amin

    2015-01-01

    In this paper, we aim to discuss the fake journals and their advertisement and publication techniques. These types of journals mostly start and continue their activities by using the name of some indexed journals and establishing fake websites. The fake journals and publishers, while asking...... the authors for a significant amount of money for publishing their papers, have no peer-review process, publish the papers without any revision on the fake sites, and put the scientific reputation and prestige of the researchers in jeopardy. In the rest of the paper, we present some viable techniques in order...

  15. Cosmic constraint on massive neutrinos in viable f(R) gravity with producing ΛCDM background expansion

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Jianbo; Wu, Yabo; Wang, Yan; Yang, Weiqiang [Liaoning Normal University, Department of Physics, Dalian (China); Liu, Molin [Xinyang Normal University, Department of Physics, Xinyang (China)

    2016-12-15

    Tensions between several cosmic observations were found recently, such as the inconsistent values of H{sub 0} (or σ{sub 8}) were indicated by the different cosmic observations. Introducing the massive neutrinos in ΛCDM could potentially solve the tensions. Viable f(R) gravity producing ΛCDM background expansion with massive neutrinos is investigated in this paper. We fit the current observational data: Planck-2015 CMB, RSD, BAO, and SNIa to constrain the mass of neutrinos in viable f(R) theory. The constraint results at 95% confidence level are: Σm{sub ν} < 0.202 eV for the active-neutrino case, m{sub ν,sterile}{sup eff} < 0.757 eV with N{sub eff} < 3.22 for the sterile neutrino case. For the effects due to the mass of the neutrinos, the constraint results on model parameter at 95% confidence level become f{sub R0} x 10{sup -6} > -1.89 and f{sub R0} x 10{sup -6} > -2.02 for two cases, respectively. It is also shown that the fitting values of several parameters much depend on the neutrino properties, such as the cold dark matter density, the cosmological quantities at matter-radiation equality, the neutrino density and the fraction of baryonic mass in helium. Finally, the constraint result shows that the tension between direct and CMB measurements of H{sub 0} gets slightly weaker in the viable f(R) model than that in the base ΛCDM model. (orig.)

  16. Co-generation system with integrated functions of active filter; Sistema de cogeneracion con funciones de filtro activo integradas

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez Grajales, Humberto Raul

    2002-07-15

    adaptive interference. This technique maintains the system in the best functional state, by means of continuous self adjustment facing changes in the operation parameters, generating important and satisfactory results. In addition, simultaneously the PVS uses, in the pursuit of the point of maximum power, the technique of {sup d}isturb and observe{sup ,} which offers a high performance in the injection of active power. [Spanish] Sin duda, la energia electrica es la forma de energia mas ampliamente usada en las actividades economicas y sociales de nuestro mundo moderno. Por ello, se puede decir que las companias generadoras y distribuidoras de electricidad asumen dos retos de relevante preocupacion actual. Uno es generar con mayor eficiencia, menor costo y con un minimo impacto sobre el medio ambiente. El segundo es cumplir con la calidad del suministro, que ha llegado a ser un punto importante tanto para los usuarios como para las companias que distribuyen la energia electrica, y que se refleja en una distribucion y un consumo de energia mas eficiente. El trabajo de tesis que se presenta, describe un sistema fotovoltaico (SFV) interconectado a la red electrica que ayuda a superar los retos citados. Este SFV tiene la funcion de recibir la energia solar entregada por un arreglo de celdas FV; operar en el punto maximo de potencia de las celdas (para mayor aprovechamiento de la energia captada); acondicionarla a una senal alterna, dentro de los parametros tecnicos normativos para la interconexion electrica (establecidos por la compania; Comision Federal de Electricidad en Mexico); e inyectarla a la red electrica con el proposito de compensar potencia reactiva (filtrado activo) demandada por cargas lineales y no lineales, reduciendo la contaminacion de la red electrica; ademas de compensar potencia activa en los picos de demanda de energia electrica maxima. El sistema funciona como filtro activo durante todo el dia, lo que permite que la capacidad de potencia instalada por el

  17. Social Networking and Smart Technology: Viable Environmental Communication Tools…?

    Science.gov (United States)

    Montain, J.; Byrne, J. M.

    2010-12-01

    To what extent do popular social networking channels represent a viable means for disseminating information regarding environmental change to the general public? Are new forms of communication such as YouTube™, Facebook™, MySpace™ and Twitter™ and smart devices such as iPhone™ and BlackBerry™ useful and effective in terms motivating people into social action and behavioural modification; or do they simply pay ‘lip service’ to these pressing environmental issues? This project will explore the background connections between social networking and environmental communication and education; and outline why such tools might be an appropriate way to connect to a broad audience in an efficient and unconventional manner. Further, research will survey the current prevalence of reliable environmental change information on social networking Internet-based media; and finally, suggestions for improved strategies and new directions will be provided.

  18. CALIBRACIÓN E IMPLEMENTACIÓN DEL MODELO MG PARA LA ESTIMACIÓN DE CAUDALES MÁXIMOS EN COLOMBIA CALIBRAÇÃO E IMPLEMENTAÇÃO DO MODELO MG PARA A ESTIMATIVA DE CAUDAIS MÁXIMOS NA COLÔMBIA CALIBRATION AND IMPLEMENTATION OF MG MODEL FOR ESTIMATING PEAK FLOWS IN COLOMBIA

    Directory of Open Access Journals (Sweden)

    Andrés Felipe Duque

    2010-12-01

    Full Text Available El propósito de este trabajo es calibrar un modelo para la estimación de caudales máximos que se adapte a las características hidrológicas, geomorfológicas y de cobertura vegetal de las cuencas colombianas. Por esta razón, se calibraron los parámetros del modelo probabilístico MG para todo el territorio colombiano, sus principales regiones geográficas y subregiones hidrológicamente homogéneas. Para la obtención de las ecuaciones analíticas del modelo MG se utilizaron los registros históricos de caudales máximos de 277 estaciones limnigráficas distribuidas a lo largo del territorio colombiano. Los resultados obtenidos aplicando el modelo MG calibrado y otros métodos comúnmente utilizados con el mismo propósito fueron comparados entre sí y se encontraron valores muy semejantes. El modelo MG calibrado en este trabajo se muestra como una opción viable para la estimación de caudales máximos en cuencas con información escasa en Colombia.O propósito deste trabalho é calibrar um modelo para a estimativa de caudais máximos que se adapte às características hidrológicas, geomorfológicas e de cobertura vegetal das bacias colombianas. Por esta razão, se calibraram os parâmetros do modelo probabilístico MG para todo o território colombiano, suas principais regiões geográficas e sub-regiões hidrologicamente homogêneas. Para a obtenção das equações analíticas do modelo MG se utilizaram os registros históricos de caudais máximos de 277 estações limnigráficas distribuídas ao longo do território colombiano. Os resultados obtidos aplicando o MG calibrado e outros métodos comumente utilizados com o mesmo propósito foram comparados entre si e se encontraram valores muito semelhantes. O modelo MG calibrado neste trabalho se amostra como uma opção viável para a estimativa de caudais máximos em bacias com informação escassa na Colômbia.The purpose of this paper is calibrate a model for estimating peak flows

  19. 40 CFR 180.1011 - Viable spores of the microorganism Bacillus thuringiensis Berliner; exemption from the...

    Science.gov (United States)

    2010-07-01

    ... EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1011 Viable spores of the... characteristics of the parent strain or contamination by other microorganisms. (3) Each lot of spore preparation... production is a Bacillus thuringiensis strain which does not produce β-exotoxin under standard manufacturing...

  20. The slowness of the judiciary: priorities for reform Morosidade do poder judiciário: prioridades para a reforma

    Directory of Open Access Journals (Sweden)

    Vera Lúcia Feil Ponciano

    2007-12-01

    Full Text Available The text treats about the increase of the litigation in Brazil after the current Constitution and, as a consequence, the slowness of the Judicial process in Brazil. It emphasizes the awakening of the brazilian society to the deficiencies of the law system structure, which caused the need of a Judiciary Reform. Criticizes the speeches that cause an overall feeling of a Judiciary crisis, without showing technical studies or viable solutions. Gives attention to the law changes. Points some priorities to put in practice the desired Judiciary Reform.Aborda sobre a explosão de litigiosidade ocorrida no Brasil a partir da atual Constituição Federal, que levou ao ponto culminante o problema da morosidade da prestação jurisdicional. Enfatiza o despertar da sociedade brasileira para a realidade da estrutura do sistema judicial, que gerou a necessidade de reforma do Judiciário. Destaca reformas legislativas efetuadas. Elenca algumas prioridades para a consecução da almejada reforma do Poder Judiciário.

  1. Economically Viable Components from Jerusalem Artichoke (Helianthus tuberosus L.) in a Biorefinery Concept

    Science.gov (United States)

    Johansson, Eva; Prade, Thomas; Angelidaki, Irini; Svensson, Sven-Erik; Newson, William R.; Gunnarsson, Ingólfur Bragi; Persson Hovmalm, Helena

    2015-01-01

    Biorefinery applications are receiving growing interest due to climatic and waste disposal issues and lack of petroleum resources. Jerusalem artichoke (Helianthus tuberosus L.) is suitable for biorefinery applications due to high biomass production and limited cultivation requirements. This paper focuses on the potential of Jerusalem artichoke as a biorefinery crop and the most viable products in such a case. The carbohydrates in the tubers were found to have potential for production of platform chemicals, e.g., succinic acid. However, economic analysis showed that production of platform chemicals as a single product was too expensive to be competitive with petrochemically produced sugars. Therefore, production of several products from the same crop is a must. Additional products are protein based ones from tubers and leaves and biogas from residues, although both are of low value and amount. High bioactive activity was found in the young leaves of the crop, and the sesquiterpene lactones are of specific interest, as other compounds from this group have shown inhibitory effects on several human diseases. Thus, future focus should be on understanding the usefulness of small molecules, to develop methods for their extraction and purification and to further develop sustainable and viable methods for the production of platform chemicals. PMID:25913379

  2. Does enterohemorrhagic Escherichia coli O157:H7 enter the viable but nonculturable state in salted salmon roe?

    Science.gov (United States)

    Makino, S I; Kii, T; Asakura, H; Shirahata, T; Ikeda, T; Takeshi, K; Itoh, K

    2000-12-01

    An outbreak caused by salted salmon roe contaminated with enterohemorrhagic Escherichia coli O157 occurred in Japan in 1998. Since about 0.75 to 1.5 viable cells were estimated to cause infection, we presumed that O157 might enter the viable but nonculturable (VNC) state in salted salmon roe and consequently that viable cell numbers might be underestimated. Although patient-originating O157 cells could not grow on agar plates after 72 h of incubation in 13% NaCl, they were resuscitated in yeast extract broth, and more than 90% of the cells were shown to be viable by fluorescent staining, suggesting that almost all of them could enter the VNC state in NaCl water. Roe-originating O157 was resistant to NaCl because it could grow on agar after 72 h of incubation in NaCl water, but about 20% of cells appeared to enter the VNC state. Therefore, germfree mice were infected with O157 to examine the resuscitation of cells in the VNC state and the retention of pathogenicity. O157 that originated in roe, but not patients, killed mice and was isolated from the intestine. However, these isolates had become sensitive to NaCl. O157 cells of roe origin incubated in normal media also killed mice and were isolated from the intestine, but they also became transiently NaCl sensitive. We therefore propose that bacterial cells might enter the VNC state under conditions of stress, such as those encountered in vivo or in high salt concentrations, and then revive when those conditions have eased. If so, the VNC state in food is potentially dangerous from a public health viewpoint and may have to be considered at the time of food inspection. Finally, the establishment of a simple recovery system for VNC cells should be established.

  3. Dissolvable tattoo sensors: from science fiction to a viable technology

    Science.gov (United States)

    Cheng, Huanyu; Yi, Ning

    2017-01-01

    Early surrealistic painting and science fiction movies have envisioned dissolvable tattoo electronic devices. In this paper, we will review the recent advances that transform that vision into a viable technology, with extended capabilities even beyond the early vision. Specifically, we focus on the discussion of a stretchable design for tattoo sensors and degradable materials for dissolvable sensors, in the form of inorganic devices with a performance comparable to modern electronics. Integration of these two technologies as well as the future developments of bio-integrated devices is also discussed. Many of the appealing ideas behind developments of these devices are drawn from nature and especially biological systems. Thus, bio-inspiration is believed to continue playing a key role in future devices for bio-integration and beyond.

  4. Dissolvable tattoo sensors: from science fiction to a viable technology

    International Nuclear Information System (INIS)

    Cheng, Huanyu; Yi, Ning

    2017-01-01

    Early surrealistic painting and science fiction movies have envisioned dissolvable tattoo electronic devices. In this paper, we will review the recent advances that transform that vision into a viable technology, with extended capabilities even beyond the early vision. Specifically, we focus on the discussion of a stretchable design for tattoo sensors and degradable materials for dissolvable sensors, in the form of inorganic devices with a performance comparable to modern electronics. Integration of these two technologies as well as the future developments of bio-integrated devices is also discussed. Many of the appealing ideas behind developments of these devices are drawn from nature and especially biological systems. Thus, bio-inspiration is believed to continue playing a key role in future devices for bio-integration and beyond. (invited comment)

  5. Membrane Lipids as Indicators for Viable Bacterial Communities Inhabiting Petroleum Systems.

    Science.gov (United States)

    Gruner, Andrea; Mangelsdorf, Kai; Vieth-Hillebrand, Andrea; Horsfield, Brian; van der Kraan, Geert M; Köhler, Thomas; Janka, Christoph; Morris, Brandon E L; Wilkes, Heinz

    2017-08-01

    Microbial activity in petroleum reservoirs has been implicated in a suite of detrimental effects including deterioration of petroleum quality, increases in oil sulfur content, biofouling of steel pipelines and other infrastructures, and well plugging. Here, we present a biogeochemical approach, using phospholipid fatty acids (PLFAs), for detecting viable bacteria in petroleum systems. Variations within the bacterial community along water flow paths (producing well, topside facilities, and injection well) can be elucidated in the field using the same technique, as shown here within oil production plants in the Molasse Basin of Upper Austria. The abundance of PLFAs is compared to total cellular numbers, as detected by qPCR of the 16S rDNA gene, to give an overall comparison between the resolutions of both methods in a true field setting. Additionally, the influence of biocide applications on lipid- and DNA-based quantification was investigated. The first oil field, Trattnach, showed significant PLFA abundances and cell numbers within the reservoir and topside facilities. In contrast, the second field (Engenfeld) showed very low PLFA levels overall, likely due to continuous treatment of the topside facilities with a glutaraldehyde-based antimicrobial. In comparison, Trattnach is dosed once per week in a batch fashion. Changes within PLFA compositions across the flow path, throughout the petroleum production plants, point to cellular adaptation within the system and may be linked to shifts in the dominance of certain bacterial types in oil reservoirs versus topside facilities. Overall, PLFA-based monitoring provides a useful tool to assess the abundance and high-level taxonomic diversity of viable microbial populations in oil production wells, topside infrastructure, pipelines, and other related facilities.

  6. Teaching general problem-solving skills is not a substitute for, or a viable addition to, teaching mathematics

    NARCIS (Netherlands)

    Sweller, John; Clark, Richard; Kirschner, Paul A.

    2010-01-01

    Sweller, J., Clark, R., & Kirschner, P. A. (2010). Teaching general problem-solving skills is not a substitute for, or a viable addition to, teaching mathematics. Notices of the American Mathematical Society, 57, 1303-1304.

  7. Serie: Madera para construcción Acabados para madera

    OpenAIRE

    Sáenz-Muñoz, Marta; Instituto Tecnológico de Costa Rica

    2012-01-01

    Durante mucho tiempo, la madera ha sido utilizada como material de construcción para resolver principalmente objetivos estructurales; sin embargo, actualmente, el uso de la madera ha tomado mucho auge en la estética de las construcciones, para brindar apariencia y calidez. En la actualidad, el mercado ofrece una amplia gama productos para dar diversos “acabados” a la madera, los cuales sirven para resaltar la belleza de algunas de ellas, realzar características como el color y la veta, o bien...

  8. Identification of Viable Helicobacter pylori in Drinking Water Supplies by Cultural and Molecular Techniques.

    Science.gov (United States)

    Santiago, Paula; Moreno, Yolanda; Ferrús, M Antonía

    2015-08-01

    Helicobacter pylori is one of the most common causes of chronic bacterial infection in humans, directly related to peptic ulcer and gastric cancer. It has been suggested that H. pylori can be acquired through different transmission routes, including water. In this study, culture and qPCR were used to detect and identify the presence of H. pylori in drinking water. Furthermore, the combined techniques PMA-qPCR and DVC-FISH were applied for detection of viable cells of H. pylori. Among 24 drinking water samples, 16 samples were positive for the presence of H. pylori, but viable cells were only detected in six samples. Characteristic colonies, covered by a mass of bacterial unspecific growth, were observed on selective agar plates from an only sample, after enrichment. The mixed culture was submitted to DVC-FISH and qPCR analysis, followed by sequencing of the amplicons. Molecular techniques confirmed the growth of H. pylori on the agar plate. Our results demonstrate for the first time that H. pylori can survive and be potentially infective in drinking water, showing that water distribution systems could be a potential route for H. pylori transmission. © 2015 John Wiley & Sons Ltd.

  9. Dye Sensitized Solar Cells for Economically Viable Photovoltaic Systems.

    Science.gov (United States)

    Jung, Hyun Suk; Lee, Jung-Kun

    2013-05-16

    TiO2 nanoparticle-based dye sensitized solar cells (DSSCs) have attracted a significant level of scientific and technological interest for their potential as economically viable photovoltaic devices. While DSSCs have multiple benefits such as material abundance, a short energy payback period, constant power output, and compatibility with flexible applications, there are still several challenges that hold back large scale commercialization. Critical factors determining the future of DSSCs involve energy conversion efficiency, long-term stability, and production cost. Continuous advancement of their long-term stability suggests that state-of-the-art DSSCs will operate for over 20 years without a significant decrease in performance. Nevertheless, key questions remain in regards to energy conversion efficiency improvements and material cost reduction. In this Perspective, the present state of the field and the ongoing efforts to address the requirements of DSSCs are summarized with views on the future of DSSCs.

  10. Fake Journals: Their Features and Some Viable Ways to Distinguishing Them.

    Science.gov (United States)

    Hemmat Esfe, Mohammad; Wongwises, Somchai; Asadi, Amin; Akbari, Mohammad

    2015-08-01

    In this paper, we aim to discuss the fake journals and their advertisement and publication techniques. These types of journals mostly start and continue their activities by using the name of some indexed journals and establishing fake websites. The fake journals and publishers, while asking the authors for a significant amount of money for publishing their papers, have no peer-review process, publish the papers without any revision on the fake sites, and put the scientific reputation and prestige of the researchers in jeopardy. In the rest of the paper, we present some viable techniques in order for researchers and students to identify these journals.

  11. Detection of viable Salmonella in ice cream by TaqMan real-time polymerase chain reaction assay combining propidium monoazide

    Directory of Open Access Journals (Sweden)

    Yuexia Wang

    2015-09-01

    Full Text Available Real-time polymerase chain reaction (PCR allows rapid detection of Salmonella in frozen dairy products, but it might cause a false positive detection result because it might amplify DNA from dead target cells as well. In this study, Salmonella-free frozen ice cream was initially inoculated with heat-killed Salmonella Typhimurium cells and stored at −18°C. Bacterial DNA extracted from the sample was amplified using TaqMan probe-based real-time PCR targeting the invA gene. Our results indicated that DNA from the dead cells remained stable in frozen ice cream for at least 20 days, and could produce fluorescence signal for real-time PCR as well. To overcome this limitation, propidium monoazide (PMA was combined with real-time PCR. PMA treatment can effectively prevent PCR amplification from heat-killed Salmonella cells in frozen ice cream. The PMA real-time PCR assay can selectively detect viable Salmonella at as low as 103 CFU/mL. Combining 18 hours of pre-enrichment with the assay allows for the detection of viable Salmonella at 100 CFU/mL and avoiding the false-positive result of dead cells. The PMA real-time PCR assay provides an alternative specifically for detection of viable Salmonella in ice cream. However, when the PMA real-time PCR assay was evaluated in ice cream subjected to frozen storage, it obviously underestimated the contamination situation of viable Salmonella, which might lead to a false negative result. According to this result, the use of enrichment prior to PMA real-time PCR analysis remains as the more appropriate approach.

  12. Detection of viable Salmonella in ice cream by TaqMan real-time polymerase chain reaction assay combining propidium monoazide.

    Science.gov (United States)

    Wang, Yuexia; Yang, Ming; Liu, Shuchun; Chen, Wanyi; Suo, Biao

    2015-09-01

    Real-time polymerase chain reaction (PCR) allows rapid detection of Salmonella in frozen dairy products, but it might cause a false positive detection result because it might amplify DNA from dead target cells as well. In this study, Salmonella-free frozen ice cream was initially inoculated with heat-killed Salmonella Typhimurium cells and stored at -18°C. Bacterial DNA extracted from the sample was amplified using TaqMan probe-based real-time PCR targeting the invA gene. Our results indicated that DNA from the dead cells remained stable in frozen ice cream for at least 20 days, and could produce fluorescence signal for real-time PCR as well. To overcome this limitation, propidium monoazide (PMA) was combined with real-time PCR. PMA treatment can effectively prevent PCR amplification from heat-killed Salmonella cells in frozen ice cream. The PMA real-time PCR assay can selectively detect viable Salmonella at as low as 10 3  CFU/mL. Combining 18 hours of pre-enrichment with the assay allows for the detection of viable Salmonella at 10 0  CFU/mL and avoiding the false-positive result of dead cells. The PMA real-time PCR assay provides an alternative specifically for detection of viable Salmonella in ice cream. However, when the PMA real-time PCR assay was evaluated in ice cream subjected to frozen storage, it obviously underestimated the contamination situation of viable Salmonella, which might lead to a false negative result. According to this result, the use of enrichment prior to PMA real-time PCR analysis remains as the more appropriate approach. Copyright © 2015. Published by Elsevier B.V.

  13. Modelo de estructura financiera para las pymes del sector metalmecánico del corredor industrial de Boyacá

    Directory of Open Access Journals (Sweden)

    Ricardo Burbano

    2006-10-01

    Full Text Available Esta investigación es un acercamiento al funcionamiento y estado actual de las Pymes (micro, pequeñas y medianas empresas del sector metalmecánico del corredor industrial de Boyacá, a través de la cual se diseña el modelo de la estructura financiera y se describe la situación económica de dichas empresas para el conocimiento de su realidad, se articulan los elementos básicos de la estructura financiera adecuadaen el modelo, teniendo en cuenta las variablesde financiación más importantes: Utilidad neta sobre patrimonio, interés de bancos, interés de terceros, interés de proveedores y el interés de los créditos de fomento. Adicionalmente, se presenta la validación del modelo propuesto a partir de la construcción de diferentes escenarios Pesimista, Optimista y más probable con la estimación de las tasas actuales del mercado financiero para el cálculo del costo promedio ponderado de Capital. Este modelo es muy útil para los empresarios de sector, pues su aplicación permitirá aumentar laproductividad de sus empresas como estrategia viable de competitividad ante el fenómeno de la liberación de mercados que vive actualmente Colombia y el mundo.

  14. Viabilidade técnica e econômica da caixa Embrapa para comercialização de tomate para consumo in natura Technical and economic viability of the Embrapa crate for marketing of fresh tomatoes

    Directory of Open Access Journals (Sweden)

    Nirlene J. Vilela

    2002-06-01

    Full Text Available Foram avaliadas as vantagens econômicas da nova embalagem desenvolvida pela Embrapa Hortaliças para comercialização de tomate para consumo in natura. A caixa tipo "K", embalagem mais usada no Brasil, tem grande número de características indesejáveis. Considerando os problemas da caixa tipo "K" e o alto índice de perdas pós-colheita na cadeia produtiva de hortaliças, a Embrapa Hortaliças iniciou um programa de pesquisa para o desenvolvimento de uma nova embalagem para tomate. O trabalho culminou com a definição da nova embalagem de plástico nomeada caixa Embrapa. Além de outras vantagens técnicas a nova embalagem, comparada com a caixa tipo "K", apresentou redução de perdas pós colheita (17%, considerando a conservação e integridade dos frutos. Para um horizonte de 5 anos, os indicadores econômicos para ambas embalagens, mostraram viabilidade econômica superior para a caixa Embrapa, sendo o valor presente líquido de, aproximadamente, R$ 60,7 mil e R$ 90,0 mil para a caixa tipo "K" e Caixa Embrapa, respectivamente. A taxa de benefício/custo foi de 4,80 e 7,80 para a caixa tipo "K" e caixa Embrapa, respectivamente. Considerando preços correntes e custos totais, a caixa Embrapa apresentou-se técnica e economicamente viável.The economic advantages of a new crate developed for commercialization of fresh tomatoes was evaluated. The Box type "K", name of the package for fresh vegetables crops mostly employed in Brazil, has many undesirables features. Considering the problems of box type "K" and the high index of post harvest losses in the production chain of vegetables, Embrapa Hortaliças started a research program focusing on the development of a new container for fresh tomatoes. This work ended with the adaptation of a new crate model made of plastic. This Embrapa crate showed comparative physical advantages of approximately 17% reduction in postharvest losses when compared to the box type K, considering conservation and

  15. El pago de servicios ambientales como instrumento de gestión ambiental para el abastecimiento sostenible de agua potable a la ciudad de Río Blanco, Nicaragua

    Directory of Open Access Journals (Sweden)

    Yuri Marín

    2006-02-01

    Full Text Available SE REALIZÓ UNA VALORACIÓN ECONÓMICA DE LOS RECURSOS HÍDRICOS DE LA microcuenca “La Golondrina” y se formuló una propuesta de operatoria de Pago de Servicios Ambientales para su conservación. El área de estudio corresponde al Cerro Musún, una reserva protegida bajo co-manejo, principal fuente abastecedora de agua potable a la ciudad Río Blanco. La metodología implicó: elaboración de balance hídrico para determinar la oferta hídrica, identificación de zonas críticas para la estimación de los costos ambientales; valoración contingente para estimar la disposición a pagar de la población (DAP, y revisión del marco legal e institucional que regula los recursos hídricos y las facultades de los municipios para la gestión ambiental. Los resultados del estudio indican que los problemas de la microcuenca son de calidad y no de cantidad de agua. Los costos ambientales ($26,980 dólares/año, permitirían implementar un plan de manejo para reubicación de bebederos, reforestación, letrinificación y medidas para la reconversión productiva de áreas degradadas circundantes, con sistemas agroforestales y silvopastoriles. Los ingresos esperados por DAP ($28,100 dólares/año, permiten cubrir totalmente los costos de conservación; se concluye entonces, que el Fondo Ambiental municipal resulta ser social y económicamente viable de implementar.

  16. Combined evaluation of rest-redistribution thallium-201 tomography and low-dose dobutamine echocardiography enhances the identification of viable myocardium in patients with chronic coronary artery disease

    International Nuclear Information System (INIS)

    Pace, L.; Cuocolo, A.; Salvatore, M.; Perrone-Filardi, P.; Prastaro, M.; Vezzuto, P.; Crisci, T.; Dellegrottaglie, S.; Piscione, F.; Chiariello, M.; Mainenti, P.P.; Varrone, A.

    1998-01-01

    The purpose of this study was to evaluate whether combined evaluation by discriminant analysis of rest-redistribution thallium-201 tomography and low-dose dobutamine echocardiography enhances the accuracy in identifying viable myocardium in patients with chronic coronary artery disease. Rest-redistribution 201 Tl has high sensitivity but low specificity in identifying viable myocardium, while the opposite is true for low-dose dobutamine echocardiography. Forty-six patients underwent low-dose dobutamine echocardiography and rest-redistribution 201 Tl tomography on the same day. Rest echocardiography was repeated at least 30 days (mean 40±20) after myocardial revascularization. Discriminant analysis was applied to the results of 201 Tl tomography and dobutamine echocardiography to classify a/dyskinetic segments as viable or non-viable. In 92 a/dyskinetic segments that were revascularized, rest-redistribution 201 Tl tomography yielded an accuracy of 75%, while the accuracy of dobutamine echocardiography was 70% (P 201 Tl imaging are useful and complementary techniques for identifying viable myocardium in patients with chronic coronary artery disease. Combined evaluation by discriminant analysis significantly improves accuracy, although the cost-effectiveness of such an approach remains to be determined. (orig.)

  17. Viable acrosome-intact human spermatozoa in the ejaculate as a marker of semen quality and fertility status

    DEFF Research Database (Denmark)

    Egeberg Palme, Dorte Louise; Rehfeld, Anders; Bang, Anne Kirstine

    2018-01-01

    STUDY QUESTION: Is it possible, in an unbiased and clinical relevant way, to determine the number of viable acrosome-intact human spermatozoa in ejaculates and to use this as a measure of fertility chances? SUMMARY ANSWER: Image cytometry enables easy and unbiased quantification of viable acrosome......-intact spermatozoa and it correlates with semen quality and fertility status. WHAT IS KNOWN ALREADY: The presence of the acrosome and its ability to respond to physiological inducers (e.g. progesterone) in the female reproductive tract at the appropriate time and place is required for fertilization. However......, the available assays are labor intensive and therefore not used clinically. STUDY DESIGN, SIZE, DURATION: Washed semen samples and capacitated swim-up fractions from volunteers were used to develop the assay. Subsequently washed ejaculates from patients in fertility treatment (n = 156), proven fertile men (n...

  18. ALTERNATIVA COMERCIAL PARA EXTENDER VIDA DE ANAQUEL DE PAPAYA 'MARADOL'

    Directory of Open Access Journals (Sweden)

    J. A. Osuna-García

    2009-01-01

    Full Text Available El objetivo de este trabajo fue evaluar el efecto del 1-Metilciclopropeno (1-MCP y etileno (solos o en combinación para manipular el proceso de maduración y extender vida de anaquel de papaya 'Maradol'. Durante el 2007 se realizaron tres ensayos: 1. Efecto del 1-MCP en papaya almacenada en condiciones de mercadeo y refrigeración más mercadeo; 2. Papaya tratada con 1-MCP y etileno y mezcla 1-MCP + etileno; 3. Evaluación comercial del 1-MCP y etileno en papaya. Se analizaron pérdida de peso, color externo, firmeza, color de pulpa y sólidos solubles totales. Se encontró que el 1-MCP a 100 nl·litro-1 retrasó el desarrollo de color externo y pulpa e inhibió el ablandamiento de los frutos. También se observó que el etileno a dosis de 100 ¿l·litro-1 aplicado después del 1-MCP no revirtió el efecto de éste, sin embargo, el 1-MCP aplicado después del etileno redujo la velocidad de ablandamiento y el desarrollo del color en cáscara y pulpa inducido por el etileno. Se concluye que el 1-MCP a 100 nl·litro-1 por 12 horas aplicado después del etileno puede ser una técnica viable para manipular el proceso de maduración y alargar vida de anaquel de papaya 'Maradol'.

  19. ESTUDIO DE FACTIBILIDAD PARA LA MANUFACTURA DE EMPUÑADURAS DE PET RECICLADO

    Directory of Open Access Journals (Sweden)

    Mariana Juárez N

    2011-01-01

    Full Text Available Este artículo, expone los resultados obtenidos de la investigación realizada, para proponer una nueva reutilización viable del plástico, el cual, se usa en las botellas hechas de Polietilentereftalato (PET. Este plástico cuenta con gran potencial de reciclado. Sin embargo, forma parte de las miles de toneladas que son dispuestas a los rellenos sanitarios, generando entre otras problemáticas, la necesidad de generar mayores espacios para la gestión de desechos, además del consumo de materias primas vírgenes. En este sentido, materiales con las cualidades como las del PET pueden ser reaprovechadas por medio del reciclaje, este tiene tienes varias opciones, entre estas destaca el reciclaje mecánico. Este método presenta varias ventajas, una de las más importantes es que tiene un impacto menor en el ambiente. Por lo tanto es necesaria la búsqueda de la continuación del proceso de recuperación, hasta llegar al producto terminado. Uno de los métodos de transformación, que cuentan con un ciclo corto de proceso, es la extrusión, por la cual, es posible convertir las hojuelas de PET, a un artículo de uso común, como pueden ser las empuñaduras para escobas o mechudos. Todo esto mediante un estudio previo del material, es decir, con la revisión bibliográfica de trabajos anteriores y otros medios, se encontró que el PET permite ser utilizado en diversas aplicaciones, siempre y cuando, se lleve a cabo un control minucioso del proceso durante el extruido del PET. Por tanto, este trabajo implica el aprovechamiento del PET reciclado, que contribuye a la conciencia ambiental, además de los beneficios económicos inherentes.

  20. Integración de prácticas culturales y control biológico para el manejo de Moniliophthora roreri Cif & Par

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Villamil

    2015-12-01

    Full Text Available En Colombia las pérdidas ocasionadas por Moniliophthora roreri Cif & Par en el cultivo de cacao, son considerables y los métodos de control tradicional no son suficientes. Para el manejo de la enfermedad el control biológico, surge como alternativa económicamente viable y ambientalmente sostenible. El objetivo del presente estudio fue evaluar el control de Moniliophthora roreri mediante parámetros de incidencia y severidad, utilizando aislamientos nativos y cepas comerciales de Trichoderma spp., en combinación con prácticas culturales (PC. Se evaluaron diez tratamientos, los cuales resultaron de combinar prácticas culturales, productos biológicos comerciales, aislamientos nativos de hongos y funguicidas de síntesis química. Para la evaluación, se empleó un diseño completamente aleatorio (DCA con cuatro repeticiones, para un total de 40 UE cada una compuesta por una planta del clon TCS01 (Theobroma CORPOICA la Suiza 01. Se hizo inoculación dirigida al dosel, utilizando una concentración de 1,2, 1,5 y 2,9 x107 UFC.g-1 para aislamientos nativos y 2,0 y 5,0 x108 UFC.g-1 para cepas comerciales. Se evaluó incidencia y severidad externa en los frutos durante 25 semanas. Los resultados indicaron que los frutos inoculados con el tratamiento T7 (P.C + Safer soil ®W.P y T4 (P.C + mezcla Trichoderma H20, H3, H5, presentaron los menores porcentajes de incidencia y severidad externa y los mayores rendimientos de cosecha sin diferencias significativas respecto al control químico y regional, representando una alternativa promisoria para la producción de cacao en la subregión occidental de Boyacá.

  1. Estudio de caso de un enlace de red inalambrico en una zona rural. propuesta de conexion para entidades agropecuarias

    Directory of Open Access Journals (Sweden)

    Abiel Roche Lima

    2005-07-01

    Full Text Available En este trabajo se realiza el estudio de una red inalámbrica de área local WLAN que utiliza el estándar IEEE 802.11 con topología de Enlace LAN, para dar servicios de Internet a la red del Instituto de Ciencia Animal, de Cuba, que es una entidad agropecuaria ubicado en una zona rural. A través de este enlace se resuelve uno de los principales problemas que presenta el sector agropecuario, que es la falta de comunicación entre los productores, investigadores y profesores pues, de forma general, sus entidades se encuentran ubicadas en zonas carentes de infraestructura de telecomunicaciones. Para elestudio del enlace se utilizaron varios software y variables a través del protocolo SNMP (Simple Network Management Protocol, así como del software de configuración del equipo, y se definieron diferentes condiciones ambientales y de tráfico de datos. Como resultado se obtuvo que las condiciones ambientales influyen en el Indicador de ruido por subcanales de frecuencia, pero que de forma general no afecta el funcionamiento de la red, por lo que resulta viable la utilización de esta tecnología para el enlace de entidades agropecuarias en zonas rurales. Al mismo tiempo se hace referencia al uso potencial de la tecnología de comunicación por líneas eléctricas (PLC como alternativa a utilizar para la conexión de estas entidades de forma independiente o conjuntamente con las WLAN.

  2. Sorption and precipitation of Mn2+ by viable and autoclaved Shewanella putrefaciens: Effect of contact time

    KAUST Repository

    Chubar, Natalia

    2013-01-01

    The sorption of Mn(II) by viable and inactivated cells of Shewanella putrefaciens, a non-pathogenic, facultative anaerobic, gram-negative bacterium characterised as a Mn(IV) and Fe(III) reducer, was studied under aerobic conditions, as a function of pH, bacterial density and metal loading. During a short contact time (3-24h), the adsorptive behaviour of live and dead bacteria toward Mn(II) was sufficiently similar, an observation that was reflected in the studies on adsorption kinetics at various metal loadings, effects of pH, bacteria density, isotherms and drifting of pH during adsorption. Continuing the experiment for an additional 2-30days demonstrated that the Mn(II) sorption by suspensions of viable and autoclaved cells differed significantly from one another. The sorption to dead cells was characterised by a rapid equilibration and was described by an isotherm. In contrast, the sorption (uptake) to live bacteria exhibited a complex time-dependent uptake. This uptake began as adsorption and ion exchange processes followed by bioprecipitation, and it was accompanied by the formation of polymeric sugars (EPS) and the release of dissolved organic substances. FTIR, EXAFS/XANES and XPS demonstrated that manganese(II) phosphate was the main precipitate formed in 125ml batches, which is the first evidence of the ability of microbes to synthesise manganese phosphates. XPS and XANES spectra did not detect Mn(II) oxidation. Although the release of protein-like compounds by the viable bacteria increased in the presence of Mn2+ (and, by contrast, the release of carbohydrates did not change), electrochemical analyses did not indicate any aqueous complexation of Mn(II) by the organic ligands. © 2012 Elsevier Ltd.

  3. Enhanced detection of ischemic but viable myocardium by the reinjection of thallium after stress-redistribution imaging

    International Nuclear Information System (INIS)

    Dilsizian, V.; Rocco, T.P.; Freedman, N.M.; Leon, M.B.; Bonow, R.O.

    1990-01-01

    The identification of ischemic but viable myocardium by thallium exercise scintigraphy is often imprecise, since many of the perfusion defects that develop in ischemic myocardium during exercise do not fill in on subsequent redistribution images. We hypothesized that a second injection of thallium given after the redistribution images were taken might improve the detection of ischemic but viable myocardium. We studied 100 patients with coronary artery disease, using thallium exercise tomographic imaging and radionuclide angiography. Patients received 2 mCi of thallium intravenously during exercise, redistribution imaging was performed three to four hours later, and a second dose of 1 mCi of thallium was injected at rest immediately thereafter. The three sets of images (stress, redistribution, and reinjection) were then analyzed. Ninety-two of the 100 patients had exercise-induced perfusion defects. Of the 260 abnormal myocardial regions identified by stress imaging, 85 (33 percent) appeared to be irreversible on redistribution imaging three to four hours later. However, 42 of these apparently irreversible defects (49 percent) demonstrated improved or normal thallium uptake after the second injection of thallium, with an increase in mean regional uptake from 56 +/- 12 percent on redistribution studies to 64 +/- 10 percent on reinjection imaging (P less than 0.001). Twenty patients were restudied three to six months after coronary angioplasty. Of the 15 myocardial regions with defects on redistribution studies that were identified as viable by reinjection studies before angioplasty, 13 (87 percent) had normal thallium uptake and improved regional wall motion after angioplasty. In contrast, all eight regions with persistent defects on reinjection imaging before angioplasty had abnormal thallium uptake and abnormal regional wall motion after angioplasty

  4. Construcción y calibración de un penetrómetro de impacto para medir los efectos del senderismo

    Directory of Open Access Journals (Sweden)

    Valentina Toledo Bruzual

    2016-01-01

    Full Text Available Se diseñó un penetrómetro de impacto, en forma mecánica, para evaluar la resistencia mecánica en los suelos, producida por la presión de las pisadas humanas, en dos senderos: Sabas Nieves y Los Venados, del Parque Nacional Warairo Repano (El Ávila, Venezuela. El aparato tiene la ventaja de ser ligero y por tanto, portátil, ya que solo pesa 1.2 kg. Es preciso, validado estadísticamente y práctico como lo expone el protocolo de uso. Los resultados mostraron que el uso del prototipo en campo, es sensible para detectar diferencias en la resistencia mecánica del suelo, en diferentes coberturas vegetales: Sabana de montaña y Bosque veranero, y con distintos porcentajes de materia orgánica. El equipo es confiable en cuanto a la repetibilidad de las mediciones y viable en los trabajos de investigación que se requiera realizar sobre la compactación del suelo, en los diferentes senderos del Parque Nacional Waraira Repano.

  5. Desalination of seawater with nuclear power reactors in cogeneration; Desalacion de agua de mar con reactores nucleares de potencia en cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Flores E, R M

    2004-07-01

    The growing demand for energy and hydraulic resources for satisfy the domestic, industrial, agricultural activities, etc. has wakened up the interest to carry out concerning investigations to study the diverse technologies guided to increase the available hydraulic resources, as well as to the search of alternatives of electric power generation, economic and socially profitable. In this sense the possible use of the nuclear energy is examined in cogeneration to obtain electricity and drinkable water for desalination of seawater. The technologies are analysed involved in the nuclear cogeneration (desalination technology, nuclear and desalination-nuclear joining) available in the world. At the same time it is exemplified the coupling of a nuclear reactor and a process of hybrid desalination that today in day the adult offers and economic advantages. Finally, the nuclear desalination is presented as a technical and economically viable solution in regions where necessities of drinkable water are had for the urban, agricultural consumption and industrial in great scale and that for local situations it is possible to satisfy it desalinating seawater. (Author)

  6. Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis.

    Science.gov (United States)

    Singh, Satyendra K; Singh, Aloukick K; Prasad, Kashi N; Singh, Amrita; Singh, Avinash; Rai, Ravi P; Tripathi, Mukesh; Gupta, Rakesh K; Husain, Nuzhat

    2015-11-30

    Neurocysticercosis (NCC) is a parasitic infection of central nervous system (CNS). Expression of adhesion molecules, chemokines and matrix metalloproteinases (MMPs) were investigated on brain tissues surrounding viable (n=15) and degenerating cysticerci (n=15) of Taenia solium in swine by real-time RT-PCR and ELISA. Gelatin gel zymography was performed for MMPs activity. ICAM-1 (intercellular adhesion molecule-1), E-selectin, MIP-1α (macrophage inflammatory protein-1α), Eotaxin-1 and RANTES (regulated on activation, normal T cell expressed and secreted) were associated with degenerating cysticerci (cysts). However, VCAM-1 (vascular cell adhesion molecule-1), MCP-1 (monocyte chemotactic protein-1), MMP-2 and MMP-9 were associated with both viable and degenerating cysts. In conclusion, viable and degenerating cysticerci have different immune molecule profiles and role of these molecules in disease pathogenesis needs to be investigated. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. A propidium monoazide–quantitative PCR method for the detection and quantification of viable Enterococcus faecalis in large-volume samples of marine waters

    KAUST Repository

    Salam, Khaled W.; El-Fadel, Mutasem E.; Barbour, Elie K.; Saikaly, Pascal

    2014-01-01

    The development of rapid detection assays of cell viability is essential for monitoring the microbiological quality of water systems. Coupling propidium monoazide with quantitative PCR (PMA-qPCR) has been successfully applied in different studies for the detection and quantification of viable cells in small-volume samples (0.25-1.00 mL), but it has not been evaluated sufficiently in marine environments or in large-volume samples. In this study, we successfully integrated blue light-emitting diodes for photoactivating PMA and membrane filtration into the PMA-qPCR assay for the rapid detection and quantification of viable Enterococcus faecalis cells in 10-mL samples of marine waters. The assay was optimized in phosphate-buffered saline and seawater, reducing the qPCR signal of heat-killed E. faecalis cells by 4 log10 and 3 log10 units, respectively. Results suggest that high total dissolved solid concentration (32 g/L) in seawater can reduce PMA activity. Optimal PMA-qPCR standard curves with a 6-log dynamic range and detection limit of 102 cells/mL were generated for quantifying viable E. faecalis cells in marine waters. The developed assay was compared with the standard membrane filter (MF) method by quantifying viable E. faecalis cells in seawater samples exposed to solar radiation. The results of the developed PMA-qPCR assay did not match that of the standard MF method. This difference in the results reflects the different physiological states of E. faecalis cells in seawater. In conclusion, the developed assay is a rapid (∼5 h) method for the quantification of viable E. faecalis cells in marine recreational waters, which should be further improved and tested in different seawater settings. © 2014 Springer-Verlag Berlin Heidelberg.

  8. A propidium monoazide–quantitative PCR method for the detection and quantification of viable Enterococcus faecalis in large-volume samples of marine waters

    KAUST Repository

    Salam, Khaled W.

    2014-08-23

    The development of rapid detection assays of cell viability is essential for monitoring the microbiological quality of water systems. Coupling propidium monoazide with quantitative PCR (PMA-qPCR) has been successfully applied in different studies for the detection and quantification of viable cells in small-volume samples (0.25-1.00 mL), but it has not been evaluated sufficiently in marine environments or in large-volume samples. In this study, we successfully integrated blue light-emitting diodes for photoactivating PMA and membrane filtration into the PMA-qPCR assay for the rapid detection and quantification of viable Enterococcus faecalis cells in 10-mL samples of marine waters. The assay was optimized in phosphate-buffered saline and seawater, reducing the qPCR signal of heat-killed E. faecalis cells by 4 log10 and 3 log10 units, respectively. Results suggest that high total dissolved solid concentration (32 g/L) in seawater can reduce PMA activity. Optimal PMA-qPCR standard curves with a 6-log dynamic range and detection limit of 102 cells/mL were generated for quantifying viable E. faecalis cells in marine waters. The developed assay was compared with the standard membrane filter (MF) method by quantifying viable E. faecalis cells in seawater samples exposed to solar radiation. The results of the developed PMA-qPCR assay did not match that of the standard MF method. This difference in the results reflects the different physiological states of E. faecalis cells in seawater. In conclusion, the developed assay is a rapid (∼5 h) method for the quantification of viable E. faecalis cells in marine recreational waters, which should be further improved and tested in different seawater settings. © 2014 Springer-Verlag Berlin Heidelberg.

  9. Radiation disinfestation: A viable technology for developing countries

    International Nuclear Information System (INIS)

    Loaharanu, P.

    1985-01-01

    Increasing food production in many countries is often offset by spoilage losses that occur at different stages after harvesting, slaughtering, or catching. The situation becomes critical in developing countries as more food is needed to feed the ever-increasing population. One of the major problems of losses of food and agricultural products during storage is insect infestation. This paper reviews some insect infestation problems of valuable crops in developing countries such as cereals, pulses, dried fish and meat, fresh and dried fruits, coffee and cocoa beans, spices, and cured tobacco leaves. Present practices of chemical fumigation to eliminate insect problems in these crops give rise to concern from the points of view of both public health and occupational safety. Irradiation technology has been shown to be as effective as other insect disinfestation methods and could provide a viable alternative for this purpose. Insects do not develop resistance to physical techniques such as heat or irradiation as they do to chemical treatments. Applications of radiation for disinfestation of food and agricultural products of importance to developing countries are discussed. The economics of radiation disinfestation of cereals and pulses, dried fish, and fresh fruits are also discussed

  10. Para-bosons and Para-fermions in Quantum Mechanics

    International Nuclear Information System (INIS)

    Cattani, M.S.D.; Fernandes, N.C.

    1982-01-01

    Within the framework of the ordinary quantum mechanics, a detailed study of the energy eigenfunctions of N identical particles using the irreducible representations of the permutation group in the Hilbert space is performed. It is shown that the para-states, as occurs with the boson and fermion states, are compatible with the postulates of quantum mechanics and with the principle of indistinguishability. A mathematical support for the existence of para-bosons and para-fermions is given. Gentile's quantum statistics is, in a certain sense, justified. (Author) [pt

  11. Advanced light water reactors: an economically viable part of the world's future energy mix

    International Nuclear Information System (INIS)

    Bruschi, H.J.

    1996-01-01

    In addition to safety and reliability, a common mission for the international nuclear industry in the 21. century will be ensure affordable electricity. At the Westinghouse Electric Corporation believe our advanced light water reactor (ALWR) design gives us the opportunity to provide the safest, most reliable, lowest cost, most competitive generation method possible for use by nations and utilities worldwide. While the safety and reliability aspects of the ALWR can be proven tangibly and are well-documented, questions have been raised about the technology's ability to work within the world's selling price range for electricity generation. For our industry's financial stability, and especially for the stability of the world's future power needs, Westinghouse has done extensive work on this issue and we are convinced we can meet the competitive challenge. We believe the ALWR can be an economically viable part of the world's future energy mix. This paper will define the competitive challenge that is being addressed by the industry and then analyze three specific areas: capital costs, operating costs, and financing costs. The hidden advantage of nuclear power in responding to these challenges will be explored, and a strong case will be made asserting that the advanced light water reactor will be able to compete in international markets with viable production costs. (authors)

  12. Ensayo de microturbinas hidráulicas para la generación de energía eléctrica en zonas desabastecidas (II)

    OpenAIRE

    Mascías de la Rica, Juan Manuel

    2012-01-01

    Este proyecto se engloba dentro del campo de las Tecnologías Apropiadas, que pretenden ofrecer una solución económica y técnicamente viable para abastecer de energía eléctrica a zonas desabastecidas de países en vías de desarrollo mediante la explotación de recursos energéticos renovables. Uno de los objetivos de este proyecto es el diseño y construcción de un banco de pruebas que simule condiciones de caudal y presión a modo de ser una simulación de las condiciones de un aprovechamiento hídr...

  13. Economically Viable Components from Jerusalem Artichoke (Helianthus tuberosus L.) in a Biorefinery Concept

    DEFF Research Database (Denmark)

    Johansson, Eva; Prade, Thomas; Angelidaki, Irini

    2015-01-01

    Biorefinery applications are receiving growing interest due to climatic and waste disposal issues and lack of petroleum resources. Jerusalem artichoke (Helianthus tuberosus L.) is suitable for biorefinery applications due to high biomass production and limited cultivation requirements. This paper...... focuses on the potential of Jerusalem artichoke as a biorefinery crop and the most viable products in such a case. The carbohydrates in the tubers were found to have potential for production of platform chemicals, e.g., succinic acid. However, economic analysis showed that production of platform chemicals...

  14. Emergency total thyroidectomy for bleeding anaplastic thyroid carcinoma: A viable option for palliation

    Directory of Open Access Journals (Sweden)

    Sunil Kumar

    2011-01-01

    Full Text Available Anaplastic thyroid carcinoma (ATC is a rare and highly aggressive thyroid neoplasm. Bleeding from tumor is an uncommon, but potentially life-threatening complication requiring sophisticated intervention facilities which are not usually available at odd hours in emergency. We report the case of a 45-year-old woman who presented with exsanguinating hemorrhage from ATC and was treated by emergency total thyroidectomy. The patient is well three months postoperatively. Emergency total thyroidectomy is a viable option for palliation in ATC presenting with bleeding.

  15. Information Warfare: using the viable system model as a framework to attack organisations

    Directory of Open Access Journals (Sweden)

    Bill Hutchinson

    2002-05-01

    Full Text Available Information is the glue in any organization. It is needed for policy, decision-making, control, and co-ordination. If an organisation's information systems are disrupted or destroyed, then damage to the whole inevitably follows. This paper uses a proven systemic, analytic framework the Viable System Model (VSM - in a functionalist mode, to analyse the vulnerabilities of an organisation's information resources to this form of aggression. It examines the tactics available, and where they can be used to effectively attack an organisation.

  16. 99Tcm-MIBI imaging with liposomal prostaglandin E1 infusion to detect viable myocardium in post-myocardial infarction patients

    International Nuclear Information System (INIS)

    Zheng Yong; Cai Hongbin; Miao Weibing; Lin Jun; Pan Runyang; Lin Haoxue

    2007-01-01

    Objective: The pharmacological efficiencies of liposomal prostaglandin E 1 (Lipo-PGE 1 ) infusion and nitrate infusion on 99 Tc m -methoxyisobutylisonitrile (MIBI) imaging detection of viable myocardium in post-myocardial infarction (MI) patients were compared in the current study. Methods: Thirty-two patients with MI underwent 99 Tc m -MIBI imaging at rest, nitrate infusion and Lipo-PGE 1 infusion respectively. The semi-quantitative analysis of 99 Tc m -MIBI imaging was carried out in 16 segments over each left ventricle. Results: Abnormal 99 Tc m -MIBI distribution in 216 segments (42.2%) at rest, 178 segments (34.8%) at nitrate infusion, and 184 segments (35.9%) at Lipo-PGE 1 infusion were identified. Among the 216 abnormal segments at rest, 89 (41.2%) and 81 (37.5%) were found improved after nitrate infusion and Lipo-PGE 1 infusion respectively. Detecting viable myocardium with Lipo-PGE 1 infusion imaging was accordant with that of nitrate infusion imaging in 89.8% of the cases. The mean uptake scores of abnormal segments at rest, nitrate infusion, and Lipo-PGE 1 infusion were 15.3 ± 3.3, 10.5 ± 1.4 (P 1 infusion imaging can be used for detection of viable myocardium in patients with MI. (authors)

  17. Analisis tecnico, socioeconomico y ambiental de la electrificación con energía solar fotovoltaica aislada para vivienda rural en Hato Corozal, Casanare, Colombia

    OpenAIRE

    Edwin Blasnilo Rúa Ramírez; Andrea Isabel Barrera Siabato; Martín Gómez Orduz

    2017-01-01

    La electrificación con energía solar fotovoltaica aislada es la opción más viable para vivienda rural, en especial si se encuentra muy distante del sistema nacional interconectado. Hato Corozal, Casanare, Colombia, es un municipio estructurado en su área rural por fincas muy alejadas entre sí. Su mayor inconveniente de desarrollo social y económico es la falta de electricidad como servicio de primera necesidad.  La gobernación de Casanare a través del fondo nacional de regalías aprobó la inst...

  18. Differential Effect of Viable Versus Necrotic Neutrophils on Mycobacterium tuberculosis Growth and Cytokine Induction in Whole Blood

    Directory of Open Access Journals (Sweden)

    David M. Lowe

    2018-04-01

    Full Text Available Neutrophils exert both positive and negative influences on the host response to tuberculosis, but the mechanisms by which these differential effects are mediated are unknown. We studied the impact of live and dead neutrophils on the control of Mycobacterium tuberculosis using a whole blood bioluminescence-based assay, and assayed supernatant cytokine concentrations using Luminex™ technology and ELISA. CD15+ granulocyte depletion from blood prior to infection with M. tuberculosis-lux impaired control of mycobacteria by 96 h, with a greater effect than depletion of CD4+, CD8+, or CD14+ cells (p < 0.001. Augmentation of blood with viable granulocytes significantly improved control of mycobacteria by 96 h (p = 0.001, but augmentation with necrotic granulocytes had the opposite effect (p = 0.01. Both augmentations decreased supernatant concentrations of tumor necrosis factor and interleukin (IL-12 p40/p70, but necrotic granulocyte augmentation also increased concentrations of IL-10, G-CSF, GM-CSF, and CCL2. Necrotic neutrophil augmentation reduced phagocytosis of FITC-labeled M. bovis BCG by all phagocytes, whereas viable neutrophil augmentation specifically reduced early uptake by CD14+ cells. The immunosuppressive effect of dead neutrophils required necrotic debris rather than supernatant. We conclude that viable neutrophils enhance control of M. tuberculosis in blood, but necrotic neutrophils have the opposite effect—the latter associated with induction of IL-10, growth factors, and chemoattractants. Our findings suggest a mechanism by which necrotic neutrophils may exert detrimental effects on the host response in active tuberculosis.

  19. Estrategia para el balance cero CO2 en un desarrollo turístico mediterráneo existente

    Directory of Open Access Journals (Sweden)

    Fernández, A.

    2011-10-01

    Full Text Available The purpose of this article is to show the carbon reduction strategy for the integrated regeneration of Playa de Palma, Palma de Mallorca. The article will describe the strategy which has been established and the measures planned in order to reach the target of zero CO2 emissions for 2050, and will provide a description of an energy model based 100% on renewable energies. The strategies include not only technological measures aimed at energy efficiency and the integration of renewable energies in buildings and the urban environment, but also other measures designed to change energy consumption patterns. In this latter respect, in addition to citizen awareness programmes, energy demand management models based on intelligent electrical networks will be implemented. The viable implementation of the strategies requires the establishment of energy business models and models for the corporate structures set up around the Palma Beach energy agency.En este artículo se describirá la estrategia que se ha establecido en el desarrollo de regeneración integral de Playa de Palma (Palma de Mallorca y medidas planificadas con el fin implantar el objetivo de emisiones Cero CO2 para el 2050 y la descripción del modelo energético basado 100% en energías renovables. Las estrategias incluyen medidas tecnológicas de eficiencia energética e integración de renovables en edificios y ámbito urbano pero también otras que inciden en la modificación de los patrones de consumo. En este sentido se plantearán además de planes de sensibilización al ciudadano, modelos de la gestión de la demanda energética apoyados en redes eléctricas inteligentes. Así mismo se establecerán los modelos de negocio energético y estructuras societarias constituidas entorno al ente energético Playa de Palma requeridos para hacer viable la implantación de las estrategias.

  20. Localization of viable, ischemic myocardium by positron-emission tomography with 11C-palmitate

    International Nuclear Information System (INIS)

    Lerch, R.A.; Ambos, H.D.; Bergmann, S.R.; Welch, M.J.; Ter-Pogossian, M.M.; Sobel, B.E.

    1981-01-01

    A study was performed to determine whether viable, but ischemic, tissue could be detected and localized in vivo based on external detection of impaired fatty acid metabolism. Accordingly, regional clearance of 11 C-palmitate was assessed by sequential PET in 15 anesthetized dogs. Clearance was consistently monoexponential from 5-15 minutes after administration of the tracer. In the absence of coronary stenosis (n = 7), clearance was homogeneous throughout the heart, with an average rate constant (k) of -0.060 +/- 0.005 min -1 (+/- SEM) and a coefficient of variation (CV) of 11.1 +/- 2.1% in each heart. Homogeneity persisted when the heart rate was increased from 84.4 +/- 6.0 to 202.7 +/- 11.5 beats/min with atropine (CV 13.2 +/- 3.5%). With left circumflex coronary stenosis (less than or equal to70% reduction in vessel diameter), homogeneity of 11 C-clearance under control conditions and with tachycardia did not differ from clearance in hearts without coronary stenosis. However, with stenosis >70% sufficient to induce ischemia without gross infarction, regional clearance of 11 C became markedly heterogeneous under control conditions (CV 28.1 +/- 5.5%, p 11 C in regions supplied by the stenotic vessel (k = -0.044 +/- 0.011 min -1 ) compared with clearance in well perfused regions (k = -0.064 +/- 0.011 min -1 , p 11 C-palmitate delineates zones of viable, ischemic myocardium that characteristically exhibit impaired oxidation of extracted fatty acid

  1. Marcadores para la Realidad Aumentada para fines educativos

    Directory of Open Access Journals (Sweden)

    María Reina Zarate Nava

    2013-12-01

    Full Text Available La Realidad Aumentada (AR es considerada por algunos expertos como la tecnología en crecimiento para el 2014, en diferentes sectores: ingeniería, educación, juegos y negocios, siendo este último el más usado en él 2013. Sin embargo en el sector educativo la AR se presenta en algunos proyectos que se han desarrollado para diferentes áreas de las ciencias, como Química, Física y Matemáticas, lo anterior con el objetivo de facilitar el proceso de Enseñanza-Aprendizaje. En el presente artículo se presenta el modelo de patrones utilizado para un Proyecto AR enfocado en Química, el cual presenta un conjunto de patrones que fueron valorados para facilitar el rastreo de los objetos y a su vez el entendimiento de algunos materiales de los laboratorios de Química I.

  2. Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture

    Directory of Open Access Journals (Sweden)

    Restu Syamsul Hadi

    2014-08-01

    Full Text Available Background Transplanted allogeneic dermal fibroblasts retain stem cell subpopulations, and are easily isolated, expanded and stored using standard techniques. Their potential for regenerative therapy of chronic wounds should be evaluated. The aim of this study was to determine allogeneic fibroblast viability in the presence of peripheral blood mononuclear cells (PBMC. Methods In this experimental study, fibroblasts were isolated from foreskin explants, expanded in the presence of serum, and stored using slow-freezing. We used one intervention group of allogeneic fibroblasts co-cultured with PBMC and 2 control groups of separate fibroblast and PBMC cultures.Fibroblasts were characterized by their collagen secretion and octamer-binding transcription factor 4 (OCT4 expression. Viability was evaluated using water soluble tetrazolium-1 (WST-1 proliferation assay. Absorbances were measured at 450 nm. Data analysis was performed by student’s paired t-test. Results Dermal fibroblasts were shown to secrete collagen, express OCT4, be recoverable after cryopreservation, and become attached to the culture dish in a co-culture with PBMC. Co-cultured and control fibroblasts had no significantly different cell viabilities (p>0.05. Calculated viable cell numbers increased 1.8 and 5.1-fold, respectively, at days 2 and 4 in vitro. Both groups showed comparable doubling times at days 2 and 4 in vitro. PBMC did not interfere with allogeneic fibroblast viability and proliferative capacity Conclusions Allogeneic fibroblasts remain viable and proliferate in the presence of host PBMC. Future research should evaluate allogeneic human dermal fibroblast competency in clinical settings. Dermal fibroblasts are a potential source for cell therapy in chronic wound management.

  3. Current progress and challenges in engineering viable artificial leaf for solar water splitting

    Directory of Open Access Journals (Sweden)

    Phuc D. Nguyen

    2017-12-01

    Full Text Available Large scale production of H2, a clean fuel, can be realized with just water and solar light energy by employing a viable energy conversion device called artificial leaf. In this tutorial review, we discuss on advances achieved recently and technical challenges remained toward the creation of such a leaf. Development of key components like catalysts for water electrolysis process and light harvester for harvesting solar energy as well as strategies being developed for assembling these components to create a complete artificial leaf will be highlighted.

  4. Índice de reflectancia solar de revestimientos verticales: potencial para la mitigación de la isla de calor urbana

    Directory of Open Access Journals (Sweden)

    Noelia Liliana Alchapar

    Full Text Available La condición artificial del medio urbano modifica el consumo de energía y el confort térmico. El incremento de las temperaturas de una ciudad en relación a las áreas periféricas conduce a la formación de una Isla de Calor Urbana. Es por ello, que trabajar sobre las propiedades termofísicas de los materiales (techos, pavimentos y fachadas es una estrategia de mitigación viable para disminuir las temperaturas de una ciudad. El objetivo principal de éste trabajo es estudiar la capacidad de 80 revestimientos para fachadas disponibles regionalmente para mitigar los efectos negativos de la Isla deCalor Urbana, mediante la determinación del Índice de Reflectancia Solar (SRI. El material que posee el comportamiento más eficiente es el Acrílico Rulato Travertino fino marfil (SRI = 100%, Temp. Sup.= 35 ºC, mientras el más ineficiente es el Acrílico Llaneado fino gris plomo (SRI = 29%, Temp. Sup.= 74 ºC. No obstante existen alternativas intermedias que resultan del efecto combinado de la composición del material, su color y textura. Esta situación demuestra que es posible mitigar el efecto de isla de calor a partir de una adecuada selección de revestimientos verticales.

  5. Generating para-water from para-hydrogen: A Gedankenexperiment.

    Science.gov (United States)

    Ivanov, Konstantin L; Bodenhausen, Geoffrey

    2018-07-01

    A novel conceptual approach is described that is based on the transfer of hyperpolarization from para-hydrogen in view of generating a population imbalance between the two spin isomers of H 2 O. The approach is analogous to SABRE (Signal Amplification By Reversible Exchange) and makes use of the transfer of spin order from para-hydrogen to H 2 O in a hypothetical organometallic complex. The spin order transfer is expected to be most efficient at avoided level crossings. The highest achievable enrichment levels of para- and ortho-water are discussed. Copyright © 2018 Elsevier Inc. All rights reserved.

  6. An economically viable space power relay system

    Science.gov (United States)

    Bekey, Ivan; Boudreault, Richard

    1999-09-01

    This paper describes and analyzes the economics of a power relay system that takes advantage of recent technological advances to implement a system that is economically viable. A series of power relay systems are described and analyzed which transport power ranging from 1,250 megawatts to 5,000 megawatts, and distribute it to receiving sites at transcontinental distances. Two classes of systems are discussed—those with a single reflector and delivering all the power to a single rectenna, and a second type which has multiple reflectors and distributes it to 10 rectenna sites, sharing power among them. It is shown that when offering electricity at prices competitive to those prevalent in developed cities in the US that a low IRR is inevitable, and economic feasibility of a business is unlikely. However, when the target market is Japan where the prevalent electricity prices are much greater, that an IRR exceeding 65% is readily attainable. This is extremely attractive to potential investors, making capitalization of a venture likely. The paper shows that the capital investment required for the system can be less than 1 per installed watt, contributing less than 0.02 /KW-hr to the cost of energy provision. Since selling prices in feasible regions range from 0.18 to over 030 $/kW-hr, these costs are but a small fraction of the operating expenses. Thus a very large IRR is possible for such a business.

  7. Keeping checkpoint/restart viable for exascale systems.

    Energy Technology Data Exchange (ETDEWEB)

    Riesen, Rolf E.; Bridges, Patrick G. (IBM Research, Ireland, Mulhuddart, Dublin); Stearley, Jon R.; Laros, James H., III; Oldfield, Ron A.; Arnold, Dorian (University of New Mexico, Albuquerque, NM); Pedretti, Kevin Thomas Tauke; Ferreira, Kurt Brian; Brightwell, Ronald Brian

    2011-09-01

    Next-generation exascale systems, those capable of performing a quintillion (10{sup 18}) operations per second, are expected to be delivered in the next 8-10 years. These systems, which will be 1,000 times faster than current systems, will be of unprecedented scale. As these systems continue to grow in size, faults will become increasingly common, even over the course of small calculations. Therefore, issues such as fault tolerance and reliability will limit application scalability. Current techniques to ensure progress across faults like checkpoint/restart, the dominant fault tolerance mechanism for the last 25 years, are increasingly problematic at the scales of future systems due to their excessive overheads. In this work, we evaluate a number of techniques to decrease the overhead of checkpoint/restart and keep this method viable for future exascale systems. More specifically, this work evaluates state-machine replication to dramatically increase the checkpoint interval (the time between successive checkpoint) and hash-based, probabilistic incremental checkpointing using graphics processing units to decrease the checkpoint commit time (the time to save one checkpoint). Using a combination of empirical analysis, modeling, and simulation, we study the costs and benefits of these approaches on a wide range of parameters. These results, which cover of number of high-performance computing capability workloads, different failure distributions, hardware mean time to failures, and I/O bandwidths, show the potential benefits of these techniques for meeting the reliability demands of future exascale platforms.

  8. Developing Viable Financing Models for Space Tourism

    Science.gov (United States)

    Eilingsfeld, F.; Schaetzler, D.

    2002-01-01

    Increasing commercialization of space services and the impending release of government's control of space access promise to make space ventures more attractive. Still, many investors shy away from going into the space tourism market as long as they do not feel secure that their return expectations will be met. First and foremost, attracting investors from the capital markets requires qualifying financing models. Based on earlier research on the cost of capital for space tourism, this paper gives a brief run-through of commercial, technical and financial due diligence aspects. After that, a closer look is taken at different valuation techniques as well as alternative ways of streamlining financials. Experience from earlier ventures has shown that the high cost of capital represents a significant challenge. Thus, the sophistication and professionalism of business plans and financial models needs to be very high. Special emphasis is given to the optimization of the debt-to-equity ratio over time. The different roles of equity and debt over a venture's life cycle are explained. Based on the latter, guidelines for the design of an optimized loan structure are given. These are then applied to simulating the financial performance of a typical space tourism venture over time, including the calculation of Weighted Average Cost of Capital (WACC) and Net Present Value (NPV). Based on a concluding sensitivity analysis, the lessons learned are presented. If applied properly, these will help to make space tourism economically viable.

  9. Selection of viable cell subpopulations from murine tumours using FACS

    International Nuclear Information System (INIS)

    Chaplin, D.J.; Durand, R.E.; Olive, P.L.

    1985-01-01

    The authors developed a technique which enables isolation of viable tumour cells subpopulation as a function of their distance from the blood supply. The basis for this separation procedure is that the fluorochrome, Hoechst 33342, as a result of its high avidity for cellular DNA, exhibits a marked diffusion/consumption gradient when it has to pass through several cell layers. As a result intravenous injection of Hoechst 33342 into tumour bearing animals, results in a heterogeneous straining pattern within the tumour with cells close to blood vessels being brightly fluorescent while those more distant are less intensely stained. Since these differences in staining intensity persist after tumour disaggregation, cells can be sorted into subpopulations on the basis of their fluorescence intensity using a fluorescence activated cell sorter. This technique offers the unique possibility of identifying the location of those cell subpopulations resistant to treatment with either radiation or chemotherapeutic drugs

  10. Plan de muestreo directo para Trialeurodes vaporariorum (Westwood (Hemiptera: Aleyrodidae en cultivos comerciales de tomate

    Directory of Open Access Journals (Sweden)

    Bernal Liliana

    2008-08-01

    Full Text Available

    Los daños ocasionados por Trialeurodes vaporariorum generan pérdidas económicas que disminuyen la producción y pueden contribuir en el alza de los precios del tomate. Para su control se acude a aplicaciones continuas de insecticidas, sin obtener resultados satisfactorios. Como alternativa de control se realizan liberaciones del parasitoide E. formosa. Sin embargo, el éxito de este sistema implica estimar la densidad de la plaga a través de un método de muestreo adecuado. Por ello, se estandarizó un plan de muestreo directo, económicamente viable, para ninfas de mosca blanca en un cultivo comercial de tomate bajo invernadero. Se determinó la incidencia de prácticas de manejo y de la fenología en la distribución espacial de ninfas. En términos generales, el muestreo se dividió en tres etapas: 1 muestreo totalmente aleatorio, entre las 5 y 7 semanas después de la siembra (sds, para plantas no divididas en tercios; 2 muestreo aleatorio, entre las 8 y 16 sds, los conteos se realizaron en hojas del tercio inferior; 3 muestreo bietápico: planta y hoja, entre las 17 y 24 sds, los conteos se efectuaron sobre hojas de los tercios medio y superior. Se encontró que prácticas culturales como el despunte influyeron en la distribución espacial de las ninfas. La implementación del muestreo directo generó un incremento en la producción del 0,06% y permitió estimar la densidad de ninfas de Trialeurodes vaporariorum en el cultivo (con precisiones de 5%, 10% y 25% como criterio para liberar E. formosa.

  11. Generation of viable progeny from dead brooders of endangered catfish Clarias magur (Hamilton, 1822

    Directory of Open Access Journals (Sweden)

    Sullip Kumar Majhi

    2017-10-01

    Full Text Available The obligatory air-breathing catfish Clarias magur is a prime candidate for aquaculture owing to its unique taste, high growth rate, and hardy nature. However, recently the IUCN has listed the species under the endangered category because the population has critically declined in the wild. The sexually mature C. magur brooders are often collected from their natural habitats for seed production in captivity. In many cases, the brooder dies due to handling injuries or confinement stress. In this study, we demonstrated that viable progeny could be generated from freshly dead sexually mature C. magur. Three hours after death, the gonads were excised, macroscopically examined and gamete viability was evaluated. Artificial fertilization was performed by mixing the sperm suspension with the eggs. Water was added after 1 min of mixing to activate the fertilization process. We observed 85%-93% fertilization success from gametes derived from dead donors as opposed to 90%-95% from those derived from live control donors. The embryos showed normal development and resulted in the generation of 88%-92% viable progeny, which was similar to the progeny derived from control donors (92%-93%. The results obtained in this study will have profound implications in enhancing the seed production of endangered C. magur and could potentially be applied to other key commercially or endangered fish species. Keywords: Biological sciences, Developmental biology, Zoology

  12. The reconstruction of f(ϕ)R and mimetic gravity from viable slow-roll inflation

    Science.gov (United States)

    Odintsov, S. D.; Oikonomou, V. K.

    2018-04-01

    In this work, we extend the bottom-up reconstruction framework of F (R) gravity to other modified gravities, and in particular for f (ϕ) R and mimetic F (R) gravities. We investigate which are the important conditions in order for the method to work, and we study several viable cosmological evolutions, focusing on the inflationary era. Particularly, for the f (ϕ) R theory case, we specify the functional form of the Hubble rate and of the scalar-to-tensor ratio as a function of the e-foldings number and accordingly, the rest of the physical quantities and also the slow-roll and the corresponding observational indices can be calculated. The same method is applied in the mimetic F (R) gravity case, and in both cases we thoroughly analyze the resulting free parameter space, in order to show that the viability of the models presented is guaranteed and secondly that there is a wide range of values of the free parameters for which the viability of the models occurs. In addition, the reconstruction method is also studied in the context of mimetic F (R) = R gravity. As we demonstrate, the resulting theory is viable, and also in this case, only the scalar-to-tensor ratio needs to be specified, since the rest follow from this condition. Finally, we discuss in brief how the reconstruction method could function for other modified gravities.

  13. Digestibilidade de alimentos protéicos e energéticos para fêmeas de beta Digestibility of protein feedstuffs and energetic feedstuffs for female beta

    Directory of Open Access Journals (Sweden)

    Jener Alexandre Sampaio Zuanon

    2007-08-01

    Full Text Available Foram avaliados os coeficientes de digestibilidade aparente de MS, PB, energia bruta (EB e EE de alimentos protéicos (farelo de soja e farinha de peixe e energéticos (fubá de milho e farelo de trigo para beta (Betta splendens. Fêmeas adultas foram alojadas em gaiolas e mantidas em dois aquários de fibra de vidro (30 peixes/aquário para alimentação e dois para coleta de fezes, ambos de formato cônico e com capacidade para 30 L. Os resultados dos coeficientes de digestibilidade aparente de MS, PB, EB e EE foram, respectivamente, de 69,43; 72,52; 67,91 e 55,50% para farelo de soja; 60,67; 51,15; 75,55 e 58,26% para farinha de peixe; 63,88; 87,16; 77,61 e 50,40% para fubá de milho; e 61,06; 93,37; 58,17 e 65,51% para farelo de trigo. Os resultados obtidos neste estudo permitem otimizar a formulação de dietas práticas balanceadas, economicamente viáveis para a espécie.Apparent digestibility coefficients of dry matter, crude protein, gross energy and ether extract from protein feeds: soybean meal and fish meal and from energy feeds: corn and wheat middlings were evaluated for Siamese fighting fish (Betta splendens. Adult female fish were stocked in cages and held in two fiberglass aquaria (30 fish/aquarium for feeding and two aquaria for collect fecal samples, both with conic shape with 30L. The results of apparent digestibility coefficients for DM, CP, GE and EE were respectively 69.43, 72.52, 67.91 and 55.50% for soybean meal; 60.67, 51.15, 75.55 and 58.26% for fish meal; 63.88, 87.16, 77.61 and 50.40% for corn and 61.06, 93.37, 58.17 and 65.51% for wheat middlings. The determined digestibility values are essential to economically viable practical diet formulation for Siamese fish fighting.

  14. Refractive index and solubility control of para-cymene solutions for index-matched fluid-structure interaction studies

    Science.gov (United States)

    Fort, Charles; Fu, Christopher D.; Weichselbaum, Noah A.; Bardet, Philippe M.

    2015-12-01

    To deploy optical diagnostics such as particle image velocimetry or planar laser-induced fluorescence (PLIF) in complex geometries, it is beneficial to use index-matched facilities. A binary mixture of para-cymene and cinnamaldehyde provides a viable option for matching the refractive index of acrylic, a common material for scaled models and test sections. This fluid is particularly appropriate for large-scale facilities and when a low-density and low-viscosity fluid is sought, such as in fluid-structure interaction studies. This binary solution has relatively low kinematic viscosity and density; its use enables the experimentalist to select operating temperature and to increase fluorescence signal in PLIF experiments. Measurements of spectral and temperature dependence of refractive index, density, and kinematic viscosity are reported. The effect of the binary mixture on solubility control of Rhodamine 6G is also characterized.

  15. Uma ferramenta para planejamento de estudos para concursos

    Directory of Open Access Journals (Sweden)

    Leonardo Pimentel

    2015-11-01

    Full Text Available De acordo com a constituição brasileira, a única forma de se tornar um empregado do governo é passar em um concurso público. O exame de conhecimento do cargo é um dos componentes mais importantes de um concurso. Estudar para esse exame é uma tarefa que requer planejamento. Um meio de obter um bom planejamento é construir uma grade de horários que relacione as matérias a serem estudadas com os respectivos horários disponíveis para o estudo. Esse tipo de problema se assemelha bastante à gerência de projetos, em que se procura estabelecer a distribuição das atividades ao longo do tempo disponível. Assim, torna-se interessante explorar a dinâmica de estudos para concursos como um projeto. O presente trabalho apresenta a ferramenta de apoio ao estudos - Passe-me, baseada no ciclo PDCA (do inglês, Plan-Do-Check-Act. Por se tratar de uma ferramenta web, o tempo de resposta para produzir uma grade de estudos é fundamental. A ferramenta faz uso de algoritmo genético para calcular a grade de horários priorizando a velocidade de resposta. A ferramenta foi avaliada em diferentes cenários de uso e obteve desempenho satisfatório durante a produção da grade de estudos.

  16. SALMONELLA SPECIE AND TOTAL VIABLE BACTERIAL LOAD IN ROASTED CHICKENS SOLD IN JOS-NIGERIA

    OpenAIRE

    Carol Okoli; Okonji M.C; Ugoh S.C; Okolo S.N; Okoli A.C; Alu A.J

    2007-01-01

    The study was to investigate for the presence of Salmonella specie and total viable aerobic bacterial load in roasted chickens sold in Jos. The study was carried out on twenty five chicken samples. No salmonella specie was isolated from the samples. However, other bacterial organisms were isolates, viz: 9(36%) of the samples yielded E.coli; 5(20%) yielded Citobacter species; 3(12%) yielded Proteus species and 6(24%) yielded Klebsiella species while 2(8%) showed no growth. An average total via...

  17. Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage

    DEFF Research Database (Denmark)

    Sjövall, Fredrik; Ehinger, Johannes K H; Marelsson, Sigurður E

    2013-01-01

    Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source...... of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further...

  18. Docencia para el logro y educación para emprender

    Directory of Open Access Journals (Sweden)

    Luis Gerardo Rodríguez Gómez

    1994-05-01

    Full Text Available RESUMEN En el primer artículo, el autor plasmó los elementos relevantes para una propuesta de educación para emprender. En este describe el desarrollo docente como expresión de una intensión, saber y un quehacer, encaminados a la formación del hombre emprendedor. El artículo se divide en tres partes. En la primera, se reseña los factores que orientan la intensión docente para emprender, a partir de un discurso y formas metodológicas establecidas con esa intención. En segundo lugar, se asume el espacio y tiempo la clase como unidad de análisis, para lograr objetivos de tipo institucional, programático y temático y en la parte final, se examina la evaluación docente y se hacen consideraciones generales para una propuesta de evaluación,  que surge al investigar la actuación del maestro.

  19. La formación del concepto de resiliencia empresarial: análisis cualitativo para el caso de los/as percebeiros/as de la cofradía de Baiona (España

    Directory of Open Access Journals (Sweden)

    Teresa García-Merino

    2015-09-01

    Full Text Available La resiliencia se plantea en dirección de empresas como una alternativa para enfrentar la turbulencia ambiental. Este concepto hace referencia a la capacidad dinámica de una empresa de enfrentarse con éxito a situaciones que ponen en riesgo su supervivencia, y, sobre la base del aprendizaje acumulado, desarrollar procesos de metamorfosis que deforman su estado previo. En este artículo revisamos cómo se ha empleado en otros campos, delimitamos el concepto, subrayamos el vínculo con las capacidades dinámicas y planteamos una aplicación práctica de resiliencia empresarial. El estudio recurre a la metodología cualitativa, con un análisis documental de contenido para el caso de los/as percebeiros/as gallegos/as de Baiona (España entre 2002 y 2012. Los resultados obtenidos reflejan el desarrollo de la resiliencia empresarial como capacidad dinámica, que ha permitido la deformación de unas estructuras para generar otras más viables. URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs1503349

  20. Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens.

    Science.gov (United States)

    Zhao, Xihong; Zhong, Junliang; Wei, Caijiao; Lin, Chii-Wann; Ding, Tian

    2017-01-01

    The viable but non-culturable (VBNC) state, a unique state in which a number of bacteria respond to adverse circumstances, was first discovered in 1982. Unfortunately, it has been reported that many foodborne pathogens can be induced to enter the VBNC state by the limiting environmental conditions during food processing and preservation, such as extreme temperatures, drying, irradiation, pulsed electric field, and high pressure stress, as well as the addition of preservatives and disinfectants. After entering the VBNC state, foodborne pathogens will introduce a serious crisis to food safety and public health because they cannot be detected using conventional plate counting techniques. This review provides an overview of the various features of the VBNC state, including the biological characteristics, induction and resuscitation factors, formation and resuscitation mechanisms, detection methods, and relationship to food safety.

  1. Minimum Viable Product and the Importance of Experimentation in Technology Startups

    Directory of Open Access Journals (Sweden)

    Dobrila Rancic Moogk

    2012-03-01

    Full Text Available Entrepreneurs are often faced with limited resources in their quest to commercialize new technology. This article presents the model of a lean startup, which can be applied to an organization regardless of its size or environment. It also emphasizes the conditions of extreme uncertainty under which the commercialization of new technology is carried out. The lean startup philosophy advocates efficient use of resources by introducing a minimum viable product to the market as soon as possible in order to test its value and the entrepreneur’s growth projections. This testing is done by running experiments that examine the metrics relevant to three distinct types of the growth. These experiments bring about accelerated learning to help reduce the uncertainty that accompanies commercialization projects, thereby bringing the resulting new technology to market faster.

  2. Mutagenic effects of gamma rays on the frequency and spectrum of chlorophyll and viable macro mutations in chilli (Capsicum annuum L.)

    International Nuclear Information System (INIS)

    Rangaiah, S.; Manjunath, A.; Puttaramanaik

    2004-01-01

    In the present study, uniform dry and healthy seeds of two cultivars of chilli namely Ceylon and Byadagi were exposed to 60 Co gamma rays at 5, 10, 15, 20, 25, 30, 35 and 40 krad doses at BARC, Mumbai. Thus there were total 18 treatments, eight different doses of gamma rays and one control in each cultivar. The M2 populations were compared with the respective control populations for chlorophyll and viable mutations. The treated populations were compared with the respective control populations for chlorophyll mutations (M2 and M3) and viable mutations (M2)' The results of the study are presented

  3. Sensitive and Specific Biomimetic Lipid Coated Microfluidics to Isolate Viable Circulating Tumor Cells and Microemboli for Cancer Detection.

    Directory of Open Access Journals (Sweden)

    Jia-Yang Chen

    Full Text Available Here we presented a simple and effective membrane mimetic microfluidic device with antibody conjugated supported lipid bilayer (SLB "smart coating" to capture viable circulating tumor cells (CTCs and circulating tumor microemboli (CTM directly from whole blood of all stage clinical cancer patients. The non-covalently bound SLB was able to promote dynamic clustering of lipid-tethered antibodies to CTC antigens and minimized non-specific blood cells retention through its non-fouling nature. A gentle flow further flushed away loosely-bound blood cells to achieve high purity of CTCs, and a stream of air foam injected disintegrate the SLB assemblies to release intact and viable CTCs from the chip. Human blood spiked cancer cell line test showed the ~95% overall efficiency to recover both CTCs and CTMs. Live/dead assay showed that at least 86% of recovered cells maintain viability. By using 2 mL of peripheral blood, the CTCs and CTMs counts of 63 healthy and colorectal cancer donors were positively correlated with the cancer progression. In summary, a simple and effective strategy utilizing biomimetic principle was developed to retrieve viable CTCs for enumeration, molecular analysis, as well as ex vivo culture over weeks. Due to the high sensitivity and specificity, it is the first time to show the high detection rates and quantity of CTCs in non-metastatic cancer patients. This work offers the values in both early cancer detection and prognosis of CTC and provides an accurate non-invasive strategy for routine clinical investigation on CTCs.

  4. Holistic approach to production systems from the perspective of lean manufacturing and viable systems model; Aproximacion holistica a los sistemas de produccion desde la perspectiva Lean Manufacturing y Modelos de Sistemas Viables

    Energy Technology Data Exchange (ETDEWEB)

    Puche Regaliza, J. C.; Costas Gual, J.

    2012-07-01

    The reductionism approach usually adopted by many organizations provokes too often a problem of sustain ability in the market over time in these organizations. To increase this sustain ability, we propose a structural and a behavioral change, in a way that the reductionism is replaced by an holistic approach appropriate to address the dynamic complexity inherent in any organization. With regard to structural change, we propose the use of Organizational Cybernetics and particularly the Viable System Model. With respect to behavior change, we propose the use of Lean Manufacturing paradigm. (Author)

  5. Parejas viables que perduran en el tiempo

    Directory of Open Access Journals (Sweden)

    Juan José Cuervo Rodríguez

    2013-01-01

    Full Text Available El presente artículo científico presenta resultados del proceso llevado a cabo en el proyecto de investigación docente "Mecanismos de autorregulación en parejas viables que perduran en el tiempo". Se soporta en una mirada compleja de la psicología basada en una epistemología de la construcción. En el ámbito metodológico, se inscribe en los estudios de terapia familiar desde una perspectiva de la comunicación humana como un todo integrado. Participaron nueve parejas. Los criterios de inclusión fueron: cinco o más años de convivencia, participación voluntaria, no presentar (ni haber presentado problemáticas especiales que ameriten intervención psicoterapéutica y la obtención de un porcentaje significativo en el uso de estrategias de comunicación asertiva en la resolución de conflictos. El método general utilizado fue el análisis de la comunicación en tarea de conversación. Los principales hallazgos señalan una estrecha relación entre el contexto de desarrollo de las parejas, la emergencia de códigos comunicacionales propios y la posibilidad de perdurar en el tiempo; también, se resalta el tipo de comunicación asertiva o constructiva, la construcción de valores como el respeto y la aceptación de las diferencias, y el deseo por vivir y construir bienestar común, como elementos constitutivos de su identidad como pareja.

  6. Determinación de condiciones para encapsulación de proteasa mediante electroatomización

    Directory of Open Access Journals (Sweden)

    Yessica Lorena Díaz

    2016-06-01

    Full Text Available La encapsulación es un método mediante el cual, sustancias bioactivas son introducidas en una matriz para evitar su pérdida y asimismo, facilitar su incorporación en diferentes productos; no obstante, durante este proceso, es preciso tener en cuenta la técnica y las condiciones de proceso para conseguir la formación de capsulas. El objetivo fue determinar las condiciones adecuadas para encapsulación de proteasa mediante electroatomización. La evaluación del proceso se realizó en función de las variables independientes: voltaje, concentración de material de recubrimiento y el flujo de alimentación; además de las variables dependientes: características del espectro y morfología. A las partículas obtenidas se les midió las características morfológicas (microscopía y de espectro (Raman. Los resultados mostraron que, las condiciones que permitieron la encapsulación fueron las realizadas a un voltaje de 13kV, empleando un material de recubrimiento con un aporte de solidos solubles totales de 55% y un flujo de alimentación de 0,1mL/h; presentando formación de cápsulas esféricas con espectros (Raman en el intervalo de 200 a 700cm-1; observando el pico más alto correspondiente a la enzima a 515cm-1 y tamaños entre 0,035 y 1,185μm. A partir de los resultados, se infiere que la electroatomización puede ser considerada como una técnica viable para la encapsulación de enzima proteasa, siempre y cuando se establezcan las condiciones de proceso que son propias de cada solución, permitiendo de esta manera el desarrollo de productos (aditivos que posteriormente puedan llegar a ser incorporados a otros.

  7. Modelación y simulación de un pervaporador acoplado a un proceso de sacarificación-fermentación para la producción de etanol

    OpenAIRE

    Jairo Antonio Cubillos-Lobo; Felipe Bustamante-Londoño; Alejandro Acosta-Cárdenas

    2015-01-01

    Actualmente, la integración de procesos es considerada una opción viable para reducir costos en la producción de etanol a partir de biomasa. Simulaciones y resultados experimentales han demostrado los beneficios de la integración de las etapas de sacarificación y fermentación y del acoplamiento del proceso de fermentación a la recuperación in-situ de etanol por pervaporación en la producción de bioetanol; sin embargo, no se han publicado estudios de la integración del proceso de sacarificació...

  8. The reconstruction of f(ϕR and mimetic gravity from viable slow-roll inflation

    Directory of Open Access Journals (Sweden)

    S.D. Odintsov

    2018-04-01

    Full Text Available In this work, we extend the bottom-up reconstruction framework of F(R gravity to other modified gravities, and in particular for f(ϕR and mimetic F(R gravities. We investigate which are the important conditions in order for the method to work, and we study several viable cosmological evolutions, focusing on the inflationary era. Particularly, for the f(ϕR theory case, we specify the functional form of the Hubble rate and of the scalar-to-tensor ratio as a function of the e-foldings number and accordingly, the rest of the physical quantities and also the slow-roll and the corresponding observational indices can be calculated. The same method is applied in the mimetic F(R gravity case, and in both cases we thoroughly analyze the resulting free parameter space, in order to show that the viability of the models presented is guaranteed and secondly that there is a wide range of values of the free parameters for which the viability of the models occurs. In addition, the reconstruction method is also studied in the context of mimetic F(R=R gravity. As we demonstrate, the resulting theory is viable, and also in this case, only the scalar-to-tensor ratio needs to be specified, since the rest follow from this condition. Finally, we discuss in brief how the reconstruction method could function for other modified gravities.

  9. Modelos para estimar consumo e exigências nutricionais para poedeiras comerciais

    OpenAIRE

    Palma Bendezu, Hilda Cristina [UNESP

    2016-01-01

    O objetivo deste trabalho foi elaborar um modelo para calcular as ingestões de aminoácidos e energia para a fase de maturação sexual e produção de ovos. As exigências de energia, aminoácidos e consumo de ração foram calculados pelo método fatorial, considerando na fase de maturação sexual a quantidade de nutrientes requerida para mantença e a quantidade de nutrientes necessários para crescimento e deposição de nutrientes corporais em cada componente do corpo (ovário, oviduto, corpo livre de ...

  10. DISEÑO DE UNA MÁQUINA PARA EL EMPAQUE DE FORRAJE DE MAÍZ PARA ENSILAJE: DOSIFICACIÓN Y COMPACTACIÓN DESIGN OF A MACHINE FOR THE PACKING OF MAIZE FORAGE FOR ENSILAGE: DOSIFICATION AND COMPACTATION

    Directory of Open Access Journals (Sweden)

    Marlon Jhair Herrera López

    2008-12-01

    Full Text Available Se presenta una propuesta para la industrialización del proceso de empaque de forraje de maíz utilizado para ensilaje, el cual es una alternativa de alimentación para el ganado. En este trabajo se describe el diseño de la máquina, construcción y pruebas del prototipo para dosificar y compactar, así como, la adición de nutrientes del forraje del maíz. Adicionalmente, se detalla la metodología empleada para determinar la fuerza de compactación requerida por unidad de área tal que permita extraer la mayor cantidad de aire, que conserve la forma del forraje y evite la pérdida de nutrientes. Después de determinar la fuerza de compactación, se calcularon las fuerzas axiales de la estructura utilizando el programa computacional Algor, se procedió a seleccionar los ángulos de la estructura y se verificaron los esfuerzos y las deformaciones en la estructura. Con el diseño obtenido es viable automatizar el proceso de dosificación y compactado, disminuyendo tiempo del proceso, y reduciendo las pérdidas por posible contaminación del producto.A new proposal of a packing machine of maize forage used for ensilage is presented in this work; the forage is used as an alternative for feeding cattle. A description of the machine design and the design, construction and tests of its prototype to dose, compact and add nutrients to the forage of the maize is given in this work. The methodology employed to determine the force required for compactation by unit area that allows extracting the greater amount of air of the bag that conserves its form and avoids the loss of nutrients. After determining the compactation force, the axial forces of the structure were calculate through the Algor software. The structural angles were selected and the stresses and deformations of the structure were also verified. With this design, the automation of the dosification and compactation process is feasible, reducing the losses due to potential product contamination.

  11. Towards a viable and just global nursing ethics.

    Science.gov (United States)

    Crigger, Nancy J

    2008-01-01

    Globalization, an outgrowth of technology, while informing us about people throughout the world, also raises our awareness of the extreme economic and social disparities that exist among nations. As part of a global discipline, nurses are vitally interested in reducing and eliminating disparities so that better health is achieved for all people. Recent literature in nursing encourages our discipline to engage more actively with social justice issues. Justice in health care is a major commitment of nursing; thus questions in the larger sphere of globalization, justice and ethics, are our discipline's questions also. Global justice, or fairness, is not an issue for some groups or institutions, but a deeper human rights issue that is a responsibility for everyone. What can we do to help reduce or eliminate the social and economic disparities that are so evident? What kind of ethical milieu is needed to address the threat that globalization imposes on justice and fairness? This article enriches the conceptualization of globalization by investigating recent work by Schweiker and Twiss. In addition, I discuss five qualities or characteristics that will facilitate the development of a viable and just global ethic. A global ethic guides all people in their response to human rights and poverty. Technology and business, two major forces in globalization that are generally considered beneficial, are critiqued as barriers to social justice and the common good.

  12. Modelos para estimar as exigências de energia metabolizável para poedeiras

    OpenAIRE

    Sakomura,Nilva Kazue; Basaglia,Roberta; Sá-Fortes,Cristina M. L.; Fernandes,João Batista K.

    2005-01-01

    Objetivou-se, com este trabalho, elaborar um modelo para estimar as exigências de energia metabolizável (EM) para poedeiras leves da linhagem Lohmann LSL, utilizando-se o método fatorial. Para determinar o efeito da temperatura sobre as exigências de EM para mantença, foram conduzidos experimentos em câmaras climáticas com temperaturas constantes de 12, 22 e 31ºC, utilizando a técnica do abate comparativo. A exigência de energia líquida para o ganho de peso foi determinada por meio da regress...

  13. The Belem Framework for Action: Harnessing the Power and Potential of Adult Learning and Education for a Viable Future

    Science.gov (United States)

    Adult Learning, 2012

    2012-01-01

    This article presents the Belem Framework for Action. This framework focuses on harnessing the power and potential of adult learning and education for a viable future. This framework begins with a preamble on adult education and towards lifelong learning.

  14. Viable suspensions of maize (Zea mays L.) pollen with exogenous DNA

    Energy Technology Data Exchange (ETDEWEB)

    Broglia, M. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dip. Innovazione

    1996-12-01

    A viable suspension of maize pollen in aqueous medium containing exogenous DNA would be a suitable tool in attempting maize genetic transformation via pollen grains by different techniques. In this work the effects of addition of DNA to hypertonic aqueous media able to preserve maize pollen viability were investigated. An almost total loss of viability was found when pollen was incubated with native DNA in water or sucrose medium due to the immediate sticking of DNA on the pollen wall. Calcium in the incubation medium avoided DNA sticking preserving pollen fertilization ability. Pre-washing of pollen in hypertonic sucrose solution was proved to remove DNA binding components from the pollen wall. PEG 20%, that is known to inhibit pollen, and silk nucleases, was also used instead of sucrose, without any reduction in the seed-set yields.

  15. Modelo de negocio para emprendedores: Estudio de caso

    Directory of Open Access Journals (Sweden)

    Ruben Macías Acosta

    2015-11-01

    Full Text Available Las empresas y los emprendedores necesitan estructurar proyectos que sean viables, por lo cual la generación de valor debe estar presente en los mismos. El modelo de negocios canvas es una herramienta que permite desarrollar estrategias adecuadas para las empresas o emprendedores que generen valor. El objetivo del artículo es dar a conocer el modelo de negocios canvas y presentar un caso de éxito del proyecto Taxiwoman mediante el uso de esta metodología. La metodología realizada en la investigación fue cualitativa, ya que solo se realizó el llenado del lienzo, no probabilísticos, y el tamaño de la muestra fue del grupo de trabajo, la unidad de análisis fue el resultado del proyecto de los estudiantes. Los resultados de la factibilidad del proyecto fueron obtenidos mediante la evaluación emitida por los jurados del Evento Nacional de Innovación Tecnológica 2014. Por lo que se concluye que el modelo de negocios canvas es una herramienta efectiva en la estructuración de los modelos de negocio, ya que considera todos los aspectos clave necesarios de un negocio. Companies and entrepreneurs need to structure projects that are viable, so generating value must be included. Companies or entrepreneurs can create value by designing their business models by using the Canvas methodology. The aim of this article is to explain the development of a business model using the Canvas methodology to design the project, presenting the success story of Taxiwoman project. The Taxiwoman project was part of several competitions of entrepreneurs in Mexico, its presentation at the National Technological Innovation Event 2014 (ENIT 2014 was outstanding; in the first phase of the event different projects from the State of Aguascalientes, in the second phase there were different projects from several States of the region. Due to the success of the competitions, it was possible to corroborate the feasibility of using the Canvas methodology for designing business

  16. Establecimiento simbiótico para la propagación y conservación de Orquídeas en condiciones ex citu en el Jardín Botánico de Boyacá, Colombia

    Directory of Open Access Journals (Sweden)

    Samantha E. Zamora N.

    2005-07-01

    capaces de establecer asociaciones simbióticas con orquídeas a nivel de la raíz (Alexopoulos, 1986. Se analizaron 326 ejemplares de orquídeas colectadas en el Cañón de Arcabuco, Boyacá, Colombia y mantenidas en el sustrato anterior como un mecanismo para la evaluación y conservación ex citu, 30 días después de la siembra, se analizó la población encontrándose nueve en estado de inflorescencia del genero Oncidium sp., Stellis sp. y las especies Masdevallia cumniculata, M. amisomorfa, Epidendrum secundum,  Odontoglosum coronarium, Miltonia warscewiczii, Eleantus cinnabarium. Al hacer el respectivo análisis del tipo de sustrato utilizado se determinó que es viable para el cultivo y propagación de las orquídeas en condiciones ex citu.

  17. Adaptação de um pulverizador de tração humana para aplicação localizada de herbicidas Adaptation of a manual spray for herbicide application at specific site

    Directory of Open Access Journals (Sweden)

    C.A. Viliotti

    2009-03-01

    Full Text Available Objetivou-se com este trabalho adaptar um pulverizador de tração humana para aplicação localizada de herbicidas. Para o desenvolvimento do circuito eletrônico, foi elaborado um programa computacional em linguagem Assembly, com informações sobre a variação da resistência elétrica no LDR (Resistor Dependente de Luz referente à radiação global incidente e refletida para quatro tipos de superfícies diferentes (plantas dicotiledôneas, plantas monocotiledôneas, palhada de trigo e solo. Concluiu-se que é possível e economicamente viável a utilização do sensor LDR para detecção de plantas daninhas.The objective of this work was to adapt a manual spray for herbicide application at specific site. For the development of the electronic circuit, a computer program in Assembly language was elaborated, with information on the variation of the electric resistance in LDR (Light Dependent Resistance, referring to the incident and reflected global radiation for four types of different surfaces (dicotyledonous plants, monocotyledonous plants, wheat straw and soil. It was concluded that it is possible and economically viable to utilize the LDR sensor to detect weeds.

  18. CUADERNOS DE EJERCICIOS PARA LA ENSEÑANZA DE LA HISTORIA. APUNTES DE UNA EXPERIENCIA

    Directory of Open Access Journals (Sweden)

    Yusdelis Reyna Acosta

    2015-01-01

    Full Text Available El artículo hace referencia a unos de los temas más recurrentes en la enseñanza de la Historia: el desarrollo del pensamiento histórico y crítico en los estudiantes, para lo cual se necesita de la implementación de proyecciones pedagógicas viables que sean capaces de situar al estudiante como protagonista del proceso de enseñanza-aprendizaje, que tengan en cuenta la utilización de variadas fuentes que le permitan la elaboración de sus propias conclusiones, puntos de vistas, que conlleven a la confrontación, la discusión y la toma de partido a favor o en contra de determinada actitud o decisión, que sea capaz de poner al estudiante en el ‟lugar del otro” y por qué no, que lo enseñe a reconstruir la historia desde el ejercicio de la investigación. Como ejemplo de ello se ofrecen actividades que forman parte de unos de los resultados del Proyecto de Investigación Clío Didáctica: la enseñanza de la Historia en la escuela, las mismas constituyen soporte esencial para la docencia, empleándose en varios momentos dentro del proceso de enseñanza-aprendizaje. Su utilización ha permitido el incremento del nivel de aprendizaje en los estudiantes, así como su grado de independencia y creatividad.

  19. Método para análise cinemática dos chutes de lactentes Método para el análisis cinemático de los pataleos de lactantes Method for the kinematic analysis of kicking movement in infants

    Directory of Open Access Journals (Sweden)

    Jocelene de Fátima Landgraf

    2013-03-01

    álisis cinemático de los pataleos de lactantes se mostró adecuada y viable, ya que éste sistema es accesible, de bajo costo y de fácil utilización para los investigadores en general.The aims of this study were to describe the method used for the kinematic analysis of kicking movements in infants and test its feasibility of use, using the Dvideow system. To adjust the method, four infants were longitudinally videotaped at ages from one to six months in the supine position. The experiment lasted two minutes, divided into two conditions: training and observation. The Dvideow system 6.3 for image analysis was used. It was verified that the use of four video cameras was more appropriate for the visibility of two markers simultaneously, allowing 3D reconstruction of motion. Moreover, 6 plumb lines were used to calibrate the system and ensure an accuracy of 2 mm. It was concluded that the use of the Dvideow system to perform kinematic analysis of the kicking movement in infants proved to be appropriate and feasible, since this system is accessible, of low cost and easy to be used for researchers in general.

  20. Significados da gravidez e da maternidade: discursos de primíparas e multíparas

    OpenAIRE

    Silva, Flavia Baroni; Souza, Laura Vilela e; Scorsolini-Comin, Fabio

    2013-01-01

    Resumo: O objetivo foi compreender os sentidos sobre maternidade e as vivências da gravidez em gestantes primíparas e multíparas. Foram entrevistadas seis gestantes, sendo três primíparas e três multíparas. Nas falas, foram trazidos temas como dificuldades físicas, alterações corporais, repercussão da gravidez no relacionamento conjugal, planejamento da gravidez e envolvimento paterno. Observou-se, nas primíparas, que a inexperiência trouxe insegurança, ao passo que, em algumas multíparas, ho...

  1. Estrategias para negociaciones exitosas.

    OpenAIRE

    José B. Parra V; Evelinda Santiago J.; Misael Murillo M.; Candy Atonal N.

    2010-01-01

    Las estrategias de negociación son herramientas para disminuir conflictos o para intercambiar bienes y servicios. Una negociación exitosa crea relaciones de solidaridad y propicia reciprocidad, cooperación y confianza para quienes tendrán relaciones permanentes de negociación con beneficios para ambas partes. Una estrategia de negociación exitosa requiere manejar el desarrollo del ambiente social del grupo de personas, con la finalidad de tener presente las fronteras que no pueden irrumpirse....

  2. On the use of the serial dilution culture method to enumerate viable phytoplankton in natural communities of plankton subjected to ballast water treatment.

    Science.gov (United States)

    Cullen, John J; MacIntyre, Hugh L

    2016-01-01

    Discharge standards for ballast water treatment (BWT) systems are based on concentrations of living cells, for example, as determined with vital stains. Ultraviolet radiation (UV) stops the reproduction of microorganisms without killing them outright; they are living, but not viable, and ecologically as good as dead. Consequently, UV-treated discharge can be compliant with the intent of regulation while failing a live/dead test. An alternative evaluation of BWT can be proposed based on the assessment of viable, rather than living, cells in discharge water. In principle, the serial dilution culture-most probable number (SDC-MPN) method provides the appropriate measure for phytoplankton. But, the method has been criticized, particularly because it is thought that many phytoplankton species cannot be cultured. A review of the literature shows that although SDC-MPN has been used for more than 50 years-generally to identify and count phytoplankton species that cannot be preserved-its application to enumerate total viable phytoplankton seems to be new, putting past criticisms of the method in a different light. Importantly, viable cells need to grow only enough to be detected, not to be brought into sustained culture, and competition between species in a dilution tube is irrelevant as long as the winner is detectable. Thorough consideration of sources of error leads to recommendations for minimizing and quantifying uncertainties by optimizing growth conditions and conducting systematic comparisons. We conclude that with careful evaluation, SDC-MPN is potentially an effective method for assessing the viability of phytoplankton after BWT.

  3. Interstitial Pregnancy Resulting in a Viable Infant Coexistent with Massive Perivillous Fibrin Deposition: A Case Report and Literature Review

    Directory of Open Access Journals (Sweden)

    Yusuke Tanaka

    2014-05-01

    Full Text Available Objective - The objective of this report is to describe a rare case of interstitial pregnancy ultimately resulting in a viable infant coexistent with massive perivillous fibrin deposition (MPFD. Study Design - This study is a case report and literature review. Results - A 35-year-old female patient underwent cesarean section at 32 weeks of gestation due to fetal growth restriction (FGR and breech presentation. During the operation, a diagnosis of interstitial pregnancy was established. There was no evidence of placental separation. We decided to complete surgery without removal of the placenta and waited until the placenta delivered spontaneously. The conservative management was successful, and the patient was discharged on postoperative day 13. The pathologic examination showed MPFD. Conclusion - If interstitial pregnancies are not diagnosed at an early gestational age, it can result in a viable fetus, but such pregnancies may be associated with FGR or placenta accreta.

  4. On-Farm, Almond Orchard Flooding as a Viable Aquifer Recharge Alternative

    Science.gov (United States)

    Ulrich, C.; Nico, P. S.; Wu, Y.; Newman, G. A.; Conrad, M. E.; Dahlke, H. E.

    2017-12-01

    In 2014, California legislators passed the Sustainable Groundwater Management Act (SGMA), which requires groundwater sustainability agencies (areas) to identify/prioritize water basins, develop current and projected water use/needs, develop a groundwater management plan, develop fees, etc. One of the challenges for implementing SGMA is the lack of data that can support alternative groundwater recharge methods such as on-farm flooding. Prior to anthropogenic river control, river floodplains captured excess water during overbank flow in the rainy season in the CA central valley. Today levees and canals strategically route rainy season high flows to the delta/ocean when irrigation water is not needed. Utilizing farmland once again as infiltration basins for groundwater banking and aquifer recharge could be a viable answer to California's depleted central valley aquifers. Prior to 2017, U.C. Davis had partnered with the Almond Board of California (ABC) and local growers to study the efficacy of agricultural flooding and the effects on annual almond crops (. LBNL joined this team to help understand the conveyance of recharge water, using electrical resistivity tomography (ERT), into the subsurface (i.e. localized fast paths, depth of infiltration, etc.) during flooding events. The fate of the recharge water is what is significant to understanding the viability of on-farm flooding as an aquifer recharge option. In this study two orchards (in Delhi and Modesto, CA), each approximately 2 acres, were flooded during the almond tree dormant period (January), to recharge 2 acre/ft of water into the local aquifers. ERT was used to characterize (soil structure) and monitor water infiltration over a single flooding event to investigate the fate of applied water. Data were collected every hour prior to flooding (baseline), during, and after all flood water had infiltrated (about 5 days total). Our time-lapse ERT results show a heterogeneous soil structure that leads to non

  5. Centro rural de formación en alternancia: su contribución a la formación para el trabajo de jóvenes menos favorecidos

    Directory of Open Access Journals (Sweden)

    Yudi Monge Naveros

    2016-01-01

    Full Text Available Se reporta los resultados de una investigación empírica sobre la contribución del Centro Rural de Formación en Alternancia (CRFA a la formación para el trabajo de los jóvenes menos favorecidos. Para el estudio se seleccionó un CRFA ubicado en la comunidad campesina de Occopata, de la región de Cusco, el cual imparte educación secundaria formal con un fuerte componente de formación para el trabajo. Se utilizó un enfoque metodológico mixto: cuantitativo y cualitativo, que incluyó la aplicación de encuestas, entrevistas, grupos de enf oque y observación. El estudio revela que el CRFA es una opción educativa relevante y viable para jóvenes provenientes de zonas rurales pobres. Este modelo educativo basado en la pedagogía de alternancia, promueve una formación integral y favorece el desarrollo tanto de competencias laborales, técnicas y de emprendimiento como la ampliación de capacidades y expectativas de estos jóvenes. No obstante, advierte que funciona en condiciones adversas de recursos humanos y materiales. Concluye con una relexión sobre la pertinencia de impulsar este modelo en contextos similares en Perú y dotarlo de mayores recursos a in de garantizar la equidad y calidad que requieren estos sectores vulnerables.

  6. Las energías renovables como oportunidad y desafío para el desarrollo territorial (Valle de Lerma, Salta – Argentina

    Directory of Open Access Journals (Sweden)

    Silvina Belmonte

    2014-03-01

    Full Text Available La evaluación de Energías Renovables se plantea en un contexto de Ordenamiento Territorial del Valle de Lerma (Salta como estrategia para el desarrollo regional y mejora de la calidad de vida y del ambiente. Herramientas de Evaluación Multi-Criterio, aplicaciones de Sistemas de Información Geográfica y técnicas participativas de consulta constituyen el sustento metodológico del trabajo. Los resultados se focalizan en tres puntos: diagnóstico territorial, evaluación del recurso energético y alternativas tecnológicas, y propuestas para la planificación y gestión. En el diagnóstico, las energías renovables fueron identificadas como opciones viables particularmente en cuestiones productivas y de acceso a servicios básicos. La potencialidad de los recursos renovables resultó alta en relación a la radiación solar y recurso hidráulico, moderado para el potencial eólico y de alta diversidad para el tema de biomasa. Entre las medidas de eficiencia y tecnologías evaluadas, surgieron como prioritarias: educación ambiental, planificación energética estratégica y variadas aplicaciones de energía solar (secaderos, invernaderos, arquitectura bioclimática, colectores de agua y cocinas. Entre las orientaciones para el desarrollo de políticas energéticas ‘más’ sustentables se destacaron: coordinación del sector energético con otros sectores y niveles de actuación en el marco de una planificación territorial integral, fortalecimiento de instituciones locales para la gestión energética, y superación de barreras – limitaciones a la transferencia de energías renovables y medidas de eficiencia energética a nivel local. Finalmente, en las conclusiones se plantea la importancia de valorar las energías renovables como oportunidad y desafío para promover procesos de ‘cambio’ en la región, en una concepción más comprometida con el ambiente y la sociedad.

  7. Kinetics of killing Listeria monocytogenes by macrophages: correlation of 3H-DNA release from labeled bacteria and changes in numbers of viable organisms by mathematical model

    International Nuclear Information System (INIS)

    Davies, W.A.

    1982-01-01

    Conventional methods of assessing antibacterial activities of macrophages by viable counting are limited by the precision of the statistics and are difficult to interpret quantitatively because of unrestrained extracellular growth of bacteria. An alternative technique based on the release of radioactive DNA from labeled bacteria has been offered as overcoming these drawbacks. To assess it for use with macrophages I have made a correlation with the conventional viable counting method using a mathematical model. Opsonized Listeria monocytogenes labeled with 3 H-thymidine were exposed to rat macrophages for periods up to 4 hr. Numbers of viable bacteria determined after sonication increased exponentially in the absence of live cells and this growth rate was progressively inhibited by increasing numbers of macrophages. After a lag period of 30-60 min soluble 3 H appeared in the supernatant, the amount increasing with time and numbers of macrophages. To correlate these data I developed a mathematical model that considered that changes in numbers of viable organisms were due to the difference between rates of 1) growth of extracellular bacteria and 2) killing within the macrophage. On the basis of this model curves of best fit to the viable counts data were used to predict the release of radioactivity, assuming that death of a bacterium led to the total release of its label. These predictions and the experimental data agreed well, the lag period of 30-60 min between death of the bacterium and release of radioactivity being consistent with intracellular digestion. Release of soluble radioactivity appears to be an accurate reflection of the number of bacteria killed within the macrophage

  8. Nanostructured nickel (II) phthalocyanine-MWCNTs as viable nanocomposite platform for electrocatalytic detection of asulam pesticide at neutral pH conditions

    CSIR Research Space (South Africa)

    Siswana, MP

    2010-08-01

    Full Text Available This work reports for the first time that nanostructured nickel (II) phthalocyanine/multiwalled carbon nanotubes composite supported on a basal plane pyrolytic electrode (NiPcNP/MWCNT-BPPGE) could potentially serve as a viable platform...

  9. Waste association in mass for coating formulations: a viable alternative to dispose

    International Nuclear Information System (INIS)

    Ferreira, E.A.C.; Soares Filho, J.E.; Souza, F.J.P.; Almeida, V.S. de; Oliveira, T.M. de

    2016-01-01

    The ceramic coatings industries are able to use in their formulations whose waste Eco disposal make the costly disposal, being able to reduce production costs by replacing traditional inputs for mining and industrial waste. Their raw materials are classified as plasticizers, fluxes and structural according to their physicochemical characteristics. Since waste falls within these classifications, their use in formulations becomes a viable and attractive alternative from an ecological point of view and marketing. Several studies have attested to waste incorporating viability porcelains formulations, however, is not common to find studies evaluating the addition of more than one simultaneously in formulations. It is the objective of the study, to examine whether fine waste rock and kaolin together with traditional raw materials are able to produce porcelain wet as technological properties defined by the NBR-13818. (author)

  10. Physiology limits commercially viable photoautotrophic production of microalgal biofuels.

    Science.gov (United States)

    Kenny, Philip; Flynn, Kevin J

    2017-01-01

    Algal biofuels have been offered as an alternative to fossil fuels, based on claims that microalgae can provide a highly productive source of compounds as feedstocks for sustainable transport fuels. Life cycle analyses identify algal productivity as a critical factor affecting commercial and environmental viability. Here, we use mechanistic modelling of the biological processes driving microalgal growth to explore optimal production scenarios in an industrial setting, enabling us to quantify limits to algal biofuels potential. We demonstrate how physiological and operational trade-offs combine to restrict the potential for solar-powered algal-biodiesel production in open ponds to a ceiling of ca. 8000 L ha -1 year -1 . For industrial-scale operations, practical considerations limit production to ca. 6000 L ha -1 year -1 . According to published economic models and life cycle analyses, such production rates cannot support long-term viable commercialisation of solar-powered cultivation of natural microalgae strains exclusively as feedstock for biofuels. The commercial viability of microalgal biofuels depends critically upon limitations in microalgal physiology (primarily in rates of C-fixation); we discuss the scope for addressing this bottleneck concluding that even deployment of genetically modified microalgae with radically enhanced characteristics would leave a very significant logistical if not financial burden.

  11. OBTENCIÓN IN VITRO Y CARACTERIZACIÓN DE CÉLULAS STEM DEL CORDÓN UMBILICAL HUMANO COMO ALTERNATIVA DE LAS CÉLULAS STEM DE ORIGEN EMBRIONARIO PARA LA MEDICINA REGENERATIVA

    Directory of Open Access Journals (Sweden)

    Juan Carlos Munévar Niño

    2005-01-01

    Full Text Available Durante siglos el hombre ha tratado de comprender la capacidad del cuerpo para reparar y reemplazar las células y tejidos del organismo. Después de años de trabajo dilucidando el como y el por qué de los mecanismos de reparación y regeneración tisular, los científicos se han enfocado en las células Stem. La identificación y aislamiento de células Stem de numerosos tejidos embrionarios y posnatales provee objetivos apropiados para una variedad de prácticas biotecnológicas denominadas generalmente como Medicina Regenerativa e Ingeniería Tisular. Desde el descubrimiento sobre la capacidad de las células Stem adultas para formar diferentes tipos de tejidos in vivo e in vitro, como una fuente alternativa para las células Stem embrionarias, lo que ofrece amplios potenciales terapéuticos para los seres humanos. La obtención de éstas células a partir del cordón umbilical humano es un sustituto interesante porque es un órgano fetal, fácil de obtener, descartable, lo que disminuye las dificultades bioéticas. En la Universidad El Bosque estamos aislando y caracterizando in vitro células Stem mesenquimatosas de la gelatina de Wharton del cordón umbilical de neonatos, obtenido previo consentimiento informado. Este sistema permitió obtener células precursoras viables de rápida proliferación que expresaron patrones de marcaje FGFR 3 (+, abriendo la puerta para poder diferenciarlas in vitro con fines terapéuticos.

  12. A Novel Reverse-Transcriptase Real-Time PCR Method for Quantification of Viable Vibrio Parahemolyticus in Raw Shrimp Based on a Rapid Construction of Standard Curve Method

    OpenAIRE

    Mengtong Jin; Haiquan Liu; Wenshuo Sun; Qin Li; Zhaohuan Zhang; Jibing Li; Yingjie Pan; Yong Zhao

    2015-01-01

    Vibrio parahemolyticus is an important pathogen that leads to food illness associated seafood. Therefore, rapid and reliable methods to detect and quantify the total viable V. parahaemolyticus in seafood are needed. In this assay, a RNA-based real-time reverse-transcriptase PCR (RT-qPCR) without an enrichment step has been developed for detection and quantification of the total viable V. parahaemolyticus in shrimp. RNA standards with the target segments were synthesized in vitro with T7 RNA p...

  13. Evaluation of Bacteriological Quality of Ready-to-eat Chicken Products by Total Viable Count Method

    OpenAIRE

    Ramiz Raja; Asif Iqbal; Yasir Hafiz; Mehboob Willayet; Shakoor Bhat; Mudasir Rather

    2012-01-01

    The present investigation describes the total viable count of ready-to-eat chicken products (chicken patties and chicken rolls) in Srinagar city during two seasons viz. autumn and winter. A total of 120 ready-to-eat chicken products comprising of 60 chicken patties and 60 chicken rolls were tested. The mean bacterial count of 60 chicken patties and 60 chicken rolls was 5.1281 and 4.9395 log10 cfu/g. Bacillus cereus strains were isolated from 25 of chicken patties and 22 of the chicken rolls r...

  14. Nitrate administration increases blood flow in dysfunctional but viable myocardium, leading to improved assessment of myocardial viability : A PET study

    NARCIS (Netherlands)

    Slart, Riemer H. J. A.; Agool, Ali; van Veldhuisen, Dirk J.; Dierckx, Rudi A.; Bax, Jeroen J.

    SPECT with Tc-99m-labeled agents is better able to detect viability after nitrate administration. Nitrates induce vasoclilation and may increase blood flow to severely hypoperfused but viable myocardium, thereby enhancing tracer delivery and improving the detection of viability. Quantitative data on

  15. Low-dose dobutamine stress gated SPET for identification of viable myocardium: comparison with stress-rest perfusion SPET and PET

    International Nuclear Information System (INIS)

    Yoshinaga, Keiichiro; Tamaki, Nagara; Katoh, Chietsugu; Kuge, Yuji; Noriyasu, Kazuyuki; Yamada, Satoshi; Ito, Yoshinori; Kohya, Tetsuro; Kitabatake, Akira; Kawai, Yuko

    2002-01-01

    The detection of viable myocardium is important for the prediction of functional recovery after revascularisation. However, a fixed perfusion defect often includes viable myocardium, and perfusion imaging then underestimates myocardial viability. We previously reported that low-dose dobutamine stress gated single-photon emission tomography (SPET) provides similar findings to dobutamine stress echocardiography in the assessment of myocardial viability. The present study investigated whether low-dose dobutamine stress gated SPET is of additional value as compared with stress-rest technetium-99m tetrofosmin SPET for the detection of myocardial viability. Standard stress-rest perfusion SPET, low-dose dobutamine stress gated SPET and fluorine-18 fluorodeoxyglucose positron emission tomography (FDG PET) were studied in 23 patients (mean age 67±7.6 years) with previous myocardial infarction. Twenty-one of them were successfully studied with each technique. FDG PET viability (FDG uptake ≥50%) was employed as the gold standard. One-day stress-rest 99m Tc-tetrofosmin myocardial SPET was performed. After the resting study, gated SPET was acquired following infusion of 7.5 μg kg -1 min -1 of dobutamine. Left ventricular wall motion in 16 segments was assessed by cine mode display using a four-point scale. Myocardial viability was considered present when there was improvement by one point. Of a total of 336 segments analysed, 53 had persistent defects on stress-rest perfusion SPET. FDG viability was seen in 16 of 17 dobutamine-responsive segments, but in only 11 of 36 dobutamine non-responsive segments (P<0.01). Thus, in the segments with persistent defects, viability findings on low-dose dobutamine stress gated SPET were concordant with those on FDG PET in 77% of segments (kappa value =0.55). For the detection of FDG-viable myocardium, the combination of stress-rest perfusion SPET and low-dose dobutamine stress gated SPET achieved a better sensitivity than stress

  16. Para-equilibrium phase diagrams

    International Nuclear Information System (INIS)

    Pelton, Arthur D.; Koukkari, Pertti; Pajarre, Risto; Eriksson, Gunnar

    2014-01-01

    Highlights: • A rapidly cooled system may attain a state of para-equilibrium. • In this state rapidly diffusing elements reach equilibrium but others are immobile. • Application of the Phase Rule to para-equilibrium phase diagrams is discussed. • A general algorithm to calculate para-equilibrium phase diagrams is described. - Abstract: If an initially homogeneous system at high temperature is rapidly cooled, a temporary para-equilibrium state may result in which rapidly diffusing elements have reached equilibrium but more slowly diffusing elements have remained essentially immobile. The best known example occurs when homogeneous austenite is quenched. A para-equilibrium phase assemblage may be calculated thermodynamically by Gibbs free energy minimization under the constraint that the ratios of the slowly diffusing elements are the same in all phases. Several examples of calculated para-equilibrium phase diagram sections are presented and the application of the Phase Rule is discussed. Although the rules governing the geometry of these diagrams may appear at first to be somewhat different from those for full equilibrium phase diagrams, it is shown that in fact they obey exactly the same rules with the following provision. Since the molar ratios of non-diffusing elements are the same in all phases at para-equilibrium, these ratios act, as far as the geometry of the diagram is concerned, like “potential” variables (such as T, pressure or chemical potentials) rather than like “normal” composition variables which need not be the same in all phases. A general algorithm to calculate para-equilibrium phase diagrams is presented. In the limit, if a para-equilibrium calculation is performed under the constraint that no elements diffuse, then the resultant phase diagram shows the single phase with the minimum Gibbs free energy at any point on the diagram; such calculations are of interest in physical vapor deposition when deposition is so rapid that phase

  17. Energy analysis of the production process of sugar using modern technologies of process integration; Analisis energetico del proceso de produccion del azucar utilizando tecnicas modernas de integracion de proceso

    Energy Technology Data Exchange (ETDEWEB)

    Figueroa Ibarra, Luis; Arriola Medellin, Alejandro [Instituto de Investigaciones Electricas, Temixco, Morelos (Mexico)

    1999-07-01

    The application of new concepts for the energy analysis of industrial processes, which consider the thermal integration of the process equipment, not only allows to detect energy recovery potentials that cannot be visualized with the traditional treatment of energy diagnosis of individual equipment, allows in addition, to suggest changes in the operation of the process that, without modifying the production, result in the reduction of the consumption of energy by the services and fuel. The analysis of thermal integration is illustrated for the case of a sugar mill, detecting a reduction potential in the use of fuel oil of 75%, and a potential increase of electrical production by cogeneration of 41%, departing from the modification of the use of the steam available in the process, and of the change of operation parameters of operation in the evaporation section. [Spanish] La aplicacion de nuevos conceptos para el analisis energetico de procesos industriales, los cuales consideran la integracion termica de los equipos del proceso, no unicamente permiten detectar potenciales de recuperacion de energia que no se pueden visualizar con el tratamiento tradicional de diagnostico energetico de equipos individuales, permiten ademas, sugerir cambios en la operacion del proceso que, sin modificar la produccion, redundan en una reduccion del consumo de servicios y combustible. El analisis de la integracion termica se ilustra para el caso de un ingenio azucarero, detectandose un potencial de reduccion del uso de combustoleo de 75%, y un potencial de incremento de produccion electrica por cogeneracion de 41%, a partir de la modificacion del uso del vapor disponible en el proceso, y del cambio de parametros de operacion en la seccion de evaporacion.

  18. Estandarización de un método espectrofluorométrico para medición de proteasa aspártica secretada (Sap de Candida albicans

    Directory of Open Access Journals (Sweden)

    Luz Stella Ramírez Aristizábal

    Full Text Available Introducción: la proteasa aspártica secretada (Sap es considerada un factor de virulencia en el proceso de infección por Candida albicans. La frecuencia de candidiasis a nivel mundial aumenta cada día, razón por la cual se hace necesario encontrar nuevos medicamentos que combatan esta enfermedad, al mismo tiempo desarrollar métodos que evalúen en forma rápida compuestos inhibidores de Sap. Objetivo: estandarizar un método fluorescente para identificar la inhibición de actividad de Sap. Métodos: se indujo la producción de Sap en cultivos de C. albicans según la metodología descrita por Capobiancoy se evaluaron sus niveles por electroforesis en geles SDS-PAGE en diferentes lapsos de tiempos. La actividad de Sap fue verificada por espectrofluorometría, para lo cual se determinaron las condiciones de reacción, variando las concentraciones de cobre y fluorexon, y los resultados de inhibición, se expresaron como disminución en la señal de fluorescencia. Como control de inhibición de Sap se utilizó Pepstatin A y como control positivo de actividad proteasa se utilizó pancreatina. Resultados: se establecieron concentraciones de 5,5 y 5,0 µM para fluorexon y cobre respectivamente; el tiempo óptimo de acoplamiento de estos fue de 120 min y la mayor actividad de Sap se alcanzó a las 22 h de incubación. Conclusiones: las condiciones estandarizadas para el método espectrofluorométrico, permiten confirmar la inhibición de Sap por Pepstatin A y demostrar que es un método viable para evaluar inhibidores de esta proteasa.

  19. Estrategia para elevar la motivación laboral; factor imprescindible para mejorar nuestra productividad.

    OpenAIRE

    Reina de los Ángeles Carballé Piñón

    2015-01-01

    Este trabajo "Estrategia para elevar la motivación laboral; factor imprescindible para mejorar nuestra productividad" resulta de la investigación realizada para tesis de maestría; se realizó el estudio de 2 entidades, nos permitió conocer las principales posiciones que sobre motivación existen, factores que la influencian, cómo incide en el desempeño laboral y que acciones diseñar para elevarla. Las 2 entidades seleccionadas, claves en el desempeño empresarial son un centro de interfase y un...

  20. Estrategias para negociaciones exitosas.

    Directory of Open Access Journals (Sweden)

    José B. Parra V

    2010-01-01

    Full Text Available Las estrategias de negociación son herramientas para disminuir conflictos o para intercambiar bienes y servicios. Una negociación exitosa crea relaciones de solidaridad y propicia reciprocidad, cooperación y confianza para quienes tendrán relaciones permanentes de negociación con beneficios para ambas partes. Una estrategia de negociación exitosa requiere manejar el desarrollo del ambiente social del grupo de personas, con la finalidad de tener presente las fronteras que no pueden irrumpirse. Otro aspecto importante, es el lenguaje corporal utilizado por las personas y las tácticas que se practican. El manejo de esta información permite diseñar diferentes modelos de negociaciones para cada caso específico. Este documento propone la utilización de la herramienta MONACO, diseñada por los autores, que es un software útil para encontrar los mejores tácticas posibles dentro de una negociación; basándose en el lenguaje corporal que muestra el negociante oponente durante cada oferta monetaria. El uso de este software da al negociador la oportunidad de manejar las negociaciones con mayor control, el negociador que usa el software puede elegir una estrategia de acuerdo a las relaciones que espera mantener con el negociador oponente.

  1. Formación de un banco de adn en la especie cucurbita moschata (duchesne ex lam.) duchesne ex poir y evaluación de ocho marcadores moleculares como código de barras para su autenticación

    OpenAIRE

    Cañar Serna, Dubert Yamil; Vallejo Cabrera, Franco Alirio; Muñoz Florez, Jaime Eduardo

    2014-01-01

    En Colombia, Cucurbita moschata es la especie domesticada de mayor importancia por su área de siembra, producción, versatilidad y consumo directo (Vallejo y Estrada, 2004). Como método de conservación, los bancos de ADN en plantas son el equivalente a las bibliotecas que contienen colecciones de alícuotas vegetales viables, con el propósito de mantener vivo este material y preservar sus características para el beneficio futuro de la humanidad y el medio ambiente (Painting et al., 1995). Actua...

  2. Un hidrogel de hidróxido de aluminio para eliminar el arsénico del agua

    Directory of Open Access Journals (Sweden)

    Luján Juan Carlos

    2001-01-01

    Full Text Available Objetivos. Describir la síntesis y los resultados preliminares de la aplicación de un hidrogel de hidróxido de aluminio que, agregado directamente al agua, pueda lograr la eliminación total del arsénico, cualquiera que sea la naturaleza del agua y el estado de oxidación del metaloide. Métodos. Las materias primas utilizadas para obtener el hidrogel de hidróxido de aluminio fueron: sulfato de aluminio hidratado (que se utiliza para potabilizar aguas, hipoclorito de calcio en polvo, hidróxido de amonio y agua destilada (cuando el gel se preparó a escala de laboratorio y agua de ósmosis inversa de igual o mejor calidad que el agua destilada (cuando se produjo el gel a escala piloto. El control de calidad del producto final consistió en la determinación de su capacidad para adsorber el arsénico y la realización de pruebas bacteriológicas para demostrar su esterilidad. El producto fue aplicado a muestras de agua a las que se añadió arsénico en el laboratorio y a muestras de aguas arsenicales naturales procedentes de la provincia de Tucumán, Argentina. Para el análisis del arsénico en el agua se utilizó el método colorimétrico del dietilditiocarbamato de plata. Resultados. La aplicación del hidrogel proporcionó una gran reducción del arsénico. Tanto en las aguas arsenicales naturales como en las artificiales, después del tratamiento las concentraciones de arsénico quedaron por debajo del límite de detección del método analítico utilizado (0,01 partes por millón. Las pruebas bacteriológicas del producto terminado revelaron la ausencia de bacterias viables. Conclusiones. La aplicación del hidrogel de hidróxido de aluminio proporcionó la deseada reducción de la concentración de arsénico en el agua. Este método de desarsenización es barato y fácil de utilizar en poblaciones rurales dispersas de zonas afectadas por el hidroarsenicismo que carecen de agua potable e infraestructura sanitaria y eléctrica.

  3. Sustainability and the Viable Systems Approach: Opportunities and Issues for the Governance of the Territory

    Directory of Open Access Journals (Sweden)

    Sergio Barile

    2018-03-01

    Full Text Available The aim of this paper is to propose an approach for representing the territory as a dynamic system of intersubjective relationships that is able to guarantee not only the efficiency of the processes within organizations, but also effective results in the general context and a sustainable impact on the broader environment. This contribution is developed on the basis of the viable systems approach (vSa, which is intended as a theoretical framework for the analysis of social phenomena as well as for orienting government processes. Using this theoretical framework, the proposed approach leads to the representation of the territory as a viable system that is capable of surviving in its own context by creating value for the other entities of the context (public groups of governments, communities, investors, natural environment, future generations, non-human species, thus defining the essential conditions for a sustainable equilibrium. The consideration that social phenomena have to be analyzed by taking into account the different relations and interactions that orient the behavior of individuals and, as a consequence, their main collective manifestations, i.e., organizations, underlines the importance of shifting from a traditional reductionist approach to a systemic approach. In what follows, taking a cue from the definition of sustainability that implies a wider sharing, we provide some initial critical positions, and finally shape the useful elements that can be preparatory to the introduction of a working hypothesis that is capable of delineating a possible itinerary for the development of the territory.

  4. Detection of viable but non-culturable Escherichia coli O157:H7 by PCR in combination with propidium monoazide.

    Science.gov (United States)

    Zhong, Junliang; Zhao, Xihong

    2018-01-01

    The aim of this study was to evaluate the applicability of the conventional PCR detection method combined with propidium monoazide (PMA) treatment for the detection of viable but non-culturable (VBNC) state Escherichia coli O157:H7 in ground beef meatballs. Under low temperature, E. coli O157:H7 cells were induced into the VBNC state in ground beef meatballs at - 20 °C after 152 days. The optimal PMA concentration of 5 µg/mL was obtained in beef meatball samples, which could completely inhibit the DNA amplification on dead cells (10 6  cells/mL) but with no inhibition on viable cells. The established PMA-PCR assay revealed that the VBNC counts exceeded 10 7  CFU/mL in artificial contamination beef samples, which could be used for semi-quantitative detection of VBNC cells in beef meatball samples. This study indicated that the PMA-PCR assay might be a potential method for detection of VBNC state E . coli O157:H7 cells in food products.

  5. ¿Argumentar para definir o definir para argumentar?

    OpenAIRE

    Silva, Luz; Samper, Carmen

    2013-01-01

    Se presenta y se ilustra un marco de referencia de un estudio en curso para obtener el título de Maestría en Docencia de la Matemática de la Universidad Pedagógica Nacional (Colombia); estudio sobre la conexión entre las acciones de definir y argumentar, que puede contribuir a la práctica de profesores en ejercicio y en formación. La habilidad para construir una definición es un posible indicio de comprensión, mientras que saberla de memoria no garantiza la comprensión del concepto (Vinner, 1...

  6. Viable bacteria associated with red blood cells and plasma in freshly drawn blood donations.

    Science.gov (United States)

    Damgaard, Christian; Magnussen, Karin; Enevold, Christian; Nilsson, Martin; Tolker-Nielsen, Tim; Holmstrup, Palle; Nielsen, Claus Henrik

    2015-01-01

    Infection remains a leading cause of post-transfusion mortality and morbidity. Bacterial contamination is, however, detected in less than 0.1% of blood units tested. The aim of the study was to identify viable bacteria in standard blood-pack units, with particular focus on bacteria from the oral cavity, and to determine the distribution of bacteria revealed in plasma and in the red blood cell (RBC)-fraction. Cross-sectional study. Blood were separated into plasma and RBC-suspensions, which were incubated anaerobically or aerobically for 7 days on trypticase soy blood agar (TSA) or blue lactose plates. For identification colony PCR was performed using primers targeting 16S rDNA. Blood donors attending Capital Region Blood Bank, Copenhagen University Hospital, Rigshospitalet, Hvidovre, Denmark, October 29th to December 10th 2013. 60 donors (≥50 years old), self-reported medically healthy. Bacterial growth was observed on plates inoculated with plasma or RBCs from 62% of the blood donations. Growth was evident in 21 (35%) of 60 RBC-fractions and in 32 (53%) of 60 plasma-fractions versus 8 of 60 negative controls (p = 0.005 and p = 2.6x10-6, respectively). Propionibacterium acnes was found in 23% of the donations, and Staphylococcus epidermidis in 38%. The majority of bacteria identified in the present study were either facultative anaerobic (59.5%) or anaerobic (27.8%) species, which are not likely to be detected during current routine screening. Viable bacteria are present in blood from donors self-reported as medically healthy, indicating that conventional test systems employed by blood banks insufficiently detect bacteria in plasma. Further investigation is needed to determine whether routine testing for anaerobic bacteria and testing of RBC-fractions for adherent bacteria should be recommended.

  7. Proceedings of the 24. National seminar on the rational use of energy and exposition of equipment and services and 7. Binational seminar on energy saving [Selected Papers]; 24. Seminario nacional sobre el uso racional de energia y exposicion de equipos y servicios y 7. Seminario binacional de ahorro de energia. Memoria Tecnica. [Ponencias seleccionadas

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    This document contains the technical briefings of the 24. National Seminar on the rational use of energy, and exhibition of the equipment and services and the 7. Binational seminar on energy saving, organized by the Asociacion de Profesionistas y Tecnicos en Aplicacion Energetica (ATPAE), in Ciudad Juarez, Chihuahua, Mexico, from November 9 to 11, 2005. This seminar dealt with subjects such as: electrical energy saving, energy distribution, solar energy, energy saving in compressed air, biomass, administration and control of the demand of the electrical energy, bioclimatic architecture, use of the compressed natural gas, renewable energy, co-generation, qualification and consultancy for the intelligent use of the energy in PyMES. [Spanish] Este documento contiene la memoria tecnica del 24 Seminario Nacional sobre el uso racional de energia, y exposicion de los equipos y servicios y el 7 Seminario binacional de ahorro de energia, organizado por la Asociacion de Tecnicos y Profesionistas en Aplicacion Energetica (ATPAE), en Ciudad Juarez, Chihuahua, Mexico, del 9 al 11 de noviembre del 2005. Este seminario trato temas tales como: ahorro de energia electrica, distribucion de la energia, energia solar, ahorro de energia en aire comprimido, biomasa, administracion y control de la demanda de la energia electrica, arquitectura bioclimatica, uso del gas natural comprimido, energia renovable, cogeneracion, capacitacion y consultoria para el uso inteligente de la energia en las Pymes.

  8. Guias ópticos para sensores

    OpenAIRE

    Marcos de Castro Pacitti

    1993-01-01

    Neste trabalho foram estudados guias ópticos para atuarem como sensores ou em sistemas sensores. Ênfase especial foi dada em guias ópticos retangulares, para dispositivos ópticos integrados, e fibras ópticas elípticas. A analise de fibras ópticas elípticas resultou em um modelamento pratico para projeto das mesmas em aplicações onde se deseja operar com dois modos guiados, situação esta que se mostra muito atraente para implementação de diversos tipos de sensores a fibra óptica. A seguir es...

  9. How Close We Are to Achieving Commercially Viable Large-Scale Photobiological Hydrogen Production by Cyanobacteria: A Review of the Biological Aspects

    Science.gov (United States)

    Sakurai, Hidehiro; Masukawa, Hajime; Kitashima, Masaharu; Inoue, Kazuhito

    2015-01-01

    Photobiological production of H2 by cyanobacteria is considered to be an ideal source of renewable energy because the inputs, water and sunlight, are abundant. The products of photobiological systems are H2 and O2; the H2 can be used as the energy source of fuel cells, etc., which generate electricity at high efficiencies and minimal pollution, as the waste product is H2O. Overall, production of commercially viable algal fuels in any form, including biomass and biodiesel, is challenging, and the very few systems that are operational have yet to be evaluated. In this paper we will: briefly review some of the necessary conditions for economical production, summarize the reports of photobiological H2 production by cyanobacteria, present our schemes for future production, and discuss the necessity for further progress in the research needed to achieve commercially viable large-scale H2 production. PMID:25793279

  10. Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition.

    Science.gov (United States)

    Othman, Majdiah; Ariff, Arbakariya B; Wasoh, Helmi; Kapri, Mohd Rizal; Halim, Murni

    2017-11-27

    Lactic acid bacteria are industrially important microorganisms recognized for fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Fermentation conditions such as concentration of initial glucose in the culture, concentration of lactic acid accumulated in the culture, types of pH control strategy, types of aeration mode and different agitation speed had influenced the cultivation performance of batch fermentation of Pediococcus acidilactici. The maximum viable cell concentration obtained in constant fed-batch fermentation at a feeding rate of 0.015 L/h was 6.1 times higher with 1.6 times reduction in lactic acid accumulation compared to batch fermentation. Anion exchange resin, IRA 67 was found to have the highest selectivity towards lactic acid compared to other components studied. Fed-batch fermentation of P. acidilactici coupled with lactic acid removal system using IRA 67 resin showed 55.5 and 9.1 times of improvement in maximum viable cell concentration compared to fermentation without resin for batch and fed-batch mode respectively. The improvement of the P. acidilactici growth in the constant fed-batch fermentation indicated the use of minimal and simple process control equipment is an effective approach for reducing by-product inhibition. Further improvement in the cultivation performance of P. acidilactici in fed-bath fermentation with in situ addition of anion-exchange resin significantly helped to enhance the growth of P. acidilactici by reducing the inhibitory effect of lactic acid and thus increasing probiotic production.

  11. Inclusive business for sustainable livelihoods L'entreprise au service du développement social Negocios inclusivos para medios de vida sostenibles

    Directory of Open Access Journals (Sweden)

    Virginie Bonnell

    2011-06-01

    subsistance pour les communautés à bas niveaux de revenus. La notion d'entreprise "inclusive" implique pour les sociétés de se montrer plus innovantes et de retravailler sur leur business plans. Elle implique de créer de nouveaux types d'emplois, de nouveaux marchés et des produits et services financièrement accessibles. Cette approche stimule la croissance économique et encourage l'entrepreneuriat.Cet article explique le concept d'inclusive business dans le contexte mondial actuel, en présentant quelques exemples pratiques de son application, et souligne  les différentes conditions nécessaires à la réussite des projets qui s'en inspirent. L'article présente également un outil interactif – appelé Inclusive Business Challenge. Cet outil conçu par le Conseil mondial des affaires pour le développement (WBCSD, vise à aider les entreprises et toutes les parties prenantes à identifier et mettre en œuvre différentes possibilités d'inclusive business.¿Qué relaciones existen entre negocio y desarrollo? y ¿qué función desempeña el negocio y cuáles son sus oportunidades para tratar los desafíos de la sostenibilidad de los países en desarrollo y las economías emergentes? Como motor de crecimiento y desarrollo, y para contribuir a su meta para innovar, operar y crecer, un negocio juega un papel esencial en la aceleración del progreso hacia el desarrollo.Las empresas pueden desarrollar iniciativas de negocios inclusivos, es decir, negocios sostenibles que amplíen el acceso a bienes, servicios y oportunidades de medio de vida para las comunidades con ingresos limitados de una manera comercialmente viable. El concepto de negocio inclusivo requiere una mejor atención e innovación en cuento a la forma en la que las empresas hacen sus negocios. Implica crear nuevas formas de empleo, nuevos mercados y productos y servicios asequibles. Esto estimula el crecimiento económico y fomenta la iniciativa de los negocios.Este artículo pone de relieve el concepto

  12. Inclusion of non-viable neonates in the birth record and its impact on infant mortality rates in Shelby County, Tennessee, USA

    Directory of Open Access Journals (Sweden)

    Bryan Lee Williams

    2010-02-01

    Full Text Available Rates of infant death are one of the most common indicators of a population’s overall health status. Infant mortality rates (IMRs are used to make broad inferences about the quality of health care, effects of health policies and even environmental quality. The purpose of our study was threefold: i to examine the characteristics of births in the area in relation to gestational age and birthweight; ii to estimate infant mortality using variable gestational age and/or birthweight criteria for live birth, and iii to calculate proportional mortality ratios for each cause of death using variable gestational age and/or birthweight criteria for live birth. We conducted a retrospective analysis of all Shelby County resident-linked birth and infant death certificates during the years 1999 to 2004. Descriptive test statistics were used to examine infant mortality rates in relation to specific maternal and infant risk factors. Through careful examination of 1999-2004 resident-linked birth and infant death data sets, we observed a disproportionate number of non-viable live births (≤20 weeks gestation or ≤350 grams in Shelby County. Issuance of birth certificates to these non-viable neonates is a factor that contributes to an inflated IMR. Our study demonstrates the complexity and the appropriateness of comparing infant mortality rates in smaller geographic units, given the unique characteristics of live births in Shelby County. The disproportionate number of pre-viable infants born in Shelby County greatly obfuscates neonatal mortality and de-emphasizes the importance of post-neonatal mortality.

  13. Labbtex: Toolbox para generación de informes en LATEX para Matlab

    Directory of Open Access Journals (Sweden)

    José Luis Almazán Gárate

    2012-10-01

    Full Text Available En este artículo se presenta el software desarrollado por el Equipo H3lite dentro del Departamento de Ingeneniería Civil. Transportes de la Escuela de Ingenieros de Caminos, Canales y Puertos de la Universidad Politécnica de Madrid para la generación de informes enLATEX mediante el software Matlab® y la integración en sus rutinas, Labbtex.La librería Labbtex proporciona un marco flexible para mezclar texto y código Matlab® para la generación automática de documentos. Un rchivo fuente simple contiene el texto de documentación y el código Matlab, al correr la aplicación se genera un documento final LATEX que contiene el texto, gráficos y tablas indicados con el formato de un documento LATEX. El código Matlab genera un documento LATEX usando la sintaxis. Así, LATEX (para composición de texto de alta calidad y Matlab® (para cálculo matemático pueden usarse simultáneamente. Esto permite la generación de informes en tiempo real con un uso de recursos mínimo.

  14. Thermodynamic study of residual heat from a high temperature nuclear reactor to analyze its viability in cogeneration processes; Estudio termodinamico del calor residual de un reactor nuclear de alta temperatura para analizar su viabilidad en procesos de cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Santillan R, A.; Valle H, J.; Escalante, J. A., E-mail: santillanaura@gmail.com [Universidad Politecnica Metropolitana de Hidalgo, Boulevard acceso a Tolcayuca 1009, Ex-Hacienda San Javier, 43860 Tolcayuca, Hidalgo (Mexico)

    2015-09-15

    In this paper the thermodynamic study of a nuclear power plant of high temperature at gas turbine (GTHTR300) is presented for estimating the exploitable waste heat in a process of desalination of seawater. One of the most studied and viable sustainable energy for the production of electricity, without the emission of greenhouse gases, is the nuclear energy. The fourth generation nuclear power plants have greater advantages than those currently installed plants; these advantages have to do with security, increased efficiencies and feasibility to be coupled to electrical cogeneration processes. In this paper the thermodynamic study of a nuclear power plant type GTHTR300 is realized, which is selected by greater efficiencies and have optimal conditions for use in electrical cogeneration processes due to high operating temperatures, which are between 700 and 950 degrees Celsius. The aim of the study is to determine the heat losses and the work done at each stage of the system, determining where they are the greatest losses and analyzing in that processes can be taken advantage. Based on the study was appointed that most of the energy losses are in form of heat in the coolers and usually this is emitted into the atmosphere without being used. From the results a process of desalination of seawater as electrical cogeneration process is proposed. This paper contains a brief description of the operation of the nuclear power plant, focusing on operation conditions and thermodynamic characteristics for the implementation of electrical cogeneration process, a thermodynamic analysis based on mass and energy balance was developed. The results allow quantifying the losses of thermal energy and determining the optimal section for coupling of the reactor with the desalination process, seeking to have a great overall efficiency. (Author)

  15. Monitoring viable cells of the biological control agent Lactobacillus plantarum PM411 in aerial plant surfaces by means of a strain-specific viability quantitative PCR.

    Science.gov (United States)

    Daranas, Núria; Bonaterra, Anna; Francés, Jesús; Cabrefiga, Jordi; Montesinos, Emilio; Badosa, Esther

    2018-03-09

    A viability qPCR (v-qPCR) assay was developed for the unambiguous detection and quantification of Lactobacillus plantarum PM411 viable cells in aerial plant surfaces. A 972 bp region of a PM411 predicted prophage with mosaic architecture enabled the identification of a PM411 strain-specific molecular marker. Three primer sets, with different amplicon lengths (92, 188, and 317 bp), and one TaqMan probe were designed. All the qPCR assays showed good linearity over a 4-log range and good efficiencies, but differed in sensitivity. The nucleic acid-binding dye PEMAX was used for selectively detecting and enumerating viable bacteria by v-qPCR. The primer set amplifying a 188 bp DNA fragment was selected as the most suitable for v-qPCR. The performance of the method was assessed on apple blossoms, pear, strawberry and kiwifruit leaves in potted plants under controlled environmental conditions, and pear and apple blossoms under field conditions, by comparing v-qPCR population estimation to those obtained by qPCR and specific plate counting on MRS-rifampicin. The population estimation did not differ significantly between methods when conditions were conducive to bacterial survival. However, under stressful conditions, differences between methods were observed due to cell death or viable but non-culturable state induction. While qPCR overestimated the population level, plate counting underestimated this value in comparison to v-qPCR. PM411 attained stable population levels of viable cells on flower environment under high relative humidity. However, the unfavourable conditions onto the leaf surface and the relatively dryness in the field caused an important decrease of viable population. IMPORTANCE The v-qPCR method in combination with plate counting and qPCR is a powerful tool for studies of colonization and survival in field conditions, to improve formulations and delivery strategies of PM411, or to optimize the dose and timing of spray schedules. It is expected that PEMAX

  16. Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization.

    Directory of Open Access Journals (Sweden)

    Jérémie Pourchez

    Full Text Available The incidence of Legionnaires' disease (LD in European countries and the USA has been constantly increasing since 1998. Infection of humans occurs through aerosol inhalation. To bridge the existing gap between the concentration of Legionella in a water network and the deposition of bacteria within the thoracic region (assessment of the number of viable Legionella, we validated a model mimicking realistic exposure through the use of (i recent technology for aerosol generation and (ii a 3D replicate of the human upper respiratory tract. The model's sensitivity was determined by monitoring the deposition of (i aerosolized water and Tc99m radio-aerosol as controls, and (ii bioaerosols generated from both Escherichia coli and Legionella pneumophila sg 1 suspensions. The numbers of viable Legionella prior to and after nebulization were provided by culture, flow cytometry and qPCR. This study was designed to obtain more realistic data on aerosol inhalation (vs. animal experimentation and deposition at the thoracic region in the context of LD. Upon nebulization, 40% and 48% of the initial Legionella inoculum was made of cultivable and non-cultivable cells, respectively; 0.7% of both populations reached the filter holder mimicking the thoracic region in this setup. These results are in agreement with experimental data based on quantitative microbial risk assessment methods and bring new methods that may be useful for preventing LD.

  17. Exploring viable vacua of the Z{sub 3}-symmetric NMSSM

    Energy Technology Data Exchange (ETDEWEB)

    Beuria, Jyotiranjan [Harish-Chandra Research Institute,Allahabad 211019 (India); Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai 400085 (India); Regional Centre for Accelerator-based Particle Physics, Harish-Chandra Research Institute,Allahabad 211019 (India); Chattopadhyay, Utpal [Department of Theoretical Physics, Indian Association for the Cultivation of Science, 2A & B Raja S. C. Mullick Road, Jadavpur, Kolkata 700 032 (India); Datta, AseshKrishna [Harish-Chandra Research Institute,Allahabad 211019 (India); Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai 400085 (India); Dey, Abhishek [Maulana Azad College, Government of West Bengal, 8 Rafi Ahmed Kidwai Road, Kolkata 700013 (India)

    2017-04-05

    We explore the vacua of the Z{sub 3}-symmetric Next-to-Minimal Supersymmetric Standard Model (NMSSM) and their stability by going beyond the simplistic paradigm that works with a tree-level neutral scalar potential and adheres to some specific flat directions in the field space. We work in the so-called phenomenological NMSSM (pNMSSM) scenario. Also, for our purpose, we adhere to a reasonably ‘natural’ setup by requiring |μ{sub eff}| not too large. Key effects are demonstrated by first studying the profiles of this potential under various circumstances of physical interest via a semi-analytical approach. The results thereof are compared to the ones obtained from a dedicated package like Vevacious which further incorporates the thermal effects to the potential. Regions of the pNMSSM parameter space that render the desired symmetry breaking (DSB) vacuum absolutely stable, long- or short-lived (in relation to the age of the Universe) under quantum/thermal tunneling are delineated. Regions that result in the appearance of color and charge breaking (CCB) minima are also presented. It is demonstrated that light singlet scalars along with a light LSP (lightest supersymmetric particle) having an appreciable singlino admixture are compatible with a viable DSB vacuum. Their implications for collider experiments are commented upon.

  18. Enumeration of Vibrio parahaemolyticus in the viable but nonculturable state using direct plate counts and recognition of individual gene fluorescence in situ hybridization.

    Science.gov (United States)

    Griffitt, Kimberly J; Noriea, Nicholas F; Johnson, Crystal N; Grimes, D Jay

    2011-05-01

    Vibrio parahaemolyticus is a gram-negative, halophilic bacterium indigenous to marine and estuarine environments and it is capable of causing food and water-borne illness in humans. It can also cause disease in marine animals, including cultured species. Currently, culture-based techniques are used for quantification of V. parahaemolyticus in environmental samples; however, these can be misleading as they fail to detect V. parahaemolyticus in a viable but nonculturable (VBNC) state which leads to an underestimation of the population density. In this study, we used a novel fluorescence visualization technique, called recognition of individual gene fluorescence in situ hybridization (RING-FISH), which targets chromosomal DNA for enumeration. A polynucleotide probe labeled with Cyanine 3 (Cy3) was created corresponding to the ubiquitous V. parahaemolyticus gene that codes for thermolabile hemolysin (tlh). When coupled with the Kogure method to distinguish viable from dead cells, RING-FISH probes reliably enumerated total, viable V. parahaemolyticus. The probe was tested for sensitivity and specificity against a pure culture of tlh(+), tdh(-), trh(-)V. parahaemolyticus, pure cultures of Vibrio vulnificus, Vibrio harveyi, Vibrio alginolyticus and Vibrio fischeri, and a mixed environmental sample. This research will provide additional tools for a better understanding of the risk these environmental organisms pose to human health. Copyright © 2011 Elsevier B.V. All rights reserved.

  19. Transcriptomic analysis on the formation of the viable putative non-culturable state of beer-spoilage Lactobacillus acetotolerans

    OpenAIRE

    Junyan Liu; Yang Deng; Brian M. Peters; Lin Li; Bing Li; Lequn Chen; Zhenbo Xu; Mark E. Shirtliff

    2016-01-01

    Lactic acid bacteria (LAB) are the most common beer-spoilage bacteria regardless of beer type, and thus pose significant problems for the brewery industry. The aim of this study was to investigate the genetic mechanisms involved in the ability of the hard-to-culture beer-spoilage bacterium Lactobacillus acetotolerans to enter into the viable putative non-culturable (VPNC) state. A genome-wide transcriptional analysis of beer-spoilage L. acetotolerans strains BM-LA14526, BM-LA14527, and BM-LA1...

  20. Internet como instrumento para la Yihad

    Directory of Open Access Journals (Sweden)

    Alfonso Merlos García

    2006-01-01

    Full Text Available Las extraordinarias ventajas que ofrece Internet lo han convertido en un instrumento clave y dinámico en la estrategia de los yihadistas. Los medios de comunicación, la clase política y las agencias de seguridad e información han tendido a concentrar sus esfuerzos en la neutralización del ciberterrorismo sin prestar suficiente atención a los múltiples usos de Internet que están haciendo los neosalafistas: para la propaganda y para la guerra psicológica, para el reclutamiento y para la financiación, para la documentación y para la planificación de atentados. Las democracias deberían considerar el coste que tendrá la derrota frente al movimiento yihadista globalizado en la batalla por el ciberespacio

  1. Una propuesta para el diagnóstico de fallos en sistemas industriales mediante el uso de estrategias bioinspiradas A proposal to fault diagnosis in industrial systems using bio-inspired strategies

    Directory of Open Access Journals (Sweden)

    Lídice Camps Echevarría

    2011-08-01

    Full Text Available In this work a study on the application of bio-inspired strategies for optimization to Fault Diagnosis in industrial systems is presented. The principal aim is to establish a basis for the development of new and viable model-based Fault Diagnosis Methods which improve some difficulties that the current methods cannot avoid. These difficulties are related mainly with fault sensitivity and robustness to external disturbances. In this study, there have been considered the Differential Evolution and the Ant Colony Optimization algorithms. This application is illustrated using simulated data of the Two tanks system benchmark. In order to analyze the advantages of these algorithms to obtain a diagnosis which needs to be sensitive to faults and robust to external disturbances, some experiments with incipient faults and noisy data have been simulated. The results indicate that the proposed approach, basically the combination of the two algorithms, characterizes a promising methodology for Fault Diagnosis.En el presente trabajo se presenta un estudio sobre la aplicación de estrategias bioinspiradas para la optimización al diagnóstico de fallos en sistemas industriales. El objetivo principal es establecer una base para el desarrollo de nuevos y viables métodos de diagnóstico de fallos basados en modelos que permitan mejorar las dificultades de los métodos actuales. Estas dificultades están relacionadas, fundamentalmente, con la sensibilidad ante la presencia de fallos y la robustez ante perturbaciones externas. En el estudio se consideraron los algoritmos Evolución Diferencial y Optimización por Colonia de Hormigas. La efectividad de la propuesta es analizada mediante experimentos con el conocido problema de prueba de los dos tanques. Los experimentos consideraron presencia de ruido en la información y fallos incipientes de manera que fuera posible analizar las ventajas de la propuesta en cuanto a diagnóstico robusto y sensible. Los resultados

  2. Mathematical modelling of the viable epidermis: impact of cell shape and vertical arrangement

    KAUST Repository

    Wittum, Rebecca

    2017-12-07

    In-silico methods are valuable tools for understanding the barrier function of the skin. The key benefit is that mathematical modelling allows the interplay between cell shape and function to be elucidated. This study focuses on the viable (living) epidermis. For this region, previous works suggested a diffusion model and an approximation of the cells by hexagonal prisms. The work at hand extends this in three ways. First, the extracellular space is treated with full spatial resolution. This induces a decrease of permeability by about 10%. Second, cells of tetrakaidecahedral shape are considered, in addition to the original hexagonal prisms. For both cell types, the resulting membrane permeabilities are compared. Third, for the first time, the influence of cell stacking in the vertical direction is considered. This is particularly important for the stratum granulosum, where tight junctions are present.

  3. Cross-elicitation responses to 2-methoxymethyl-para-phenylenediamine in para-phenylenediamine-allergic individuals

    NARCIS (Netherlands)

    Schuttelaar, M.-L.; Coenraads, P.-J.; Blömeke, B.; Goebel, C.

    2016-01-01

    The introduction of a methoxymethyl side chain into para-phenylenediamine (PPD) resulted in the development of a new hair dye molecule: 2-methoxymethyl-para-phenylenediamine (Me-PPD). Besides having excellent hair colouring properties, this PPD derivate has significantly lower skin-sensitizing

  4. Oro para Colombia

    OpenAIRE

    Rubio Forero, María Claudia

    2016-01-01

    Las mujeres han debido atravesar un largo camino partiendo de la discriminación hasta empezar un proceso de equidad en la sociedad y en el deporte, de esta manera llegar a ser parte de un evento como los Juegos Olímpicos. Esta tesis toma el caso de tres atletas colombianas y describe su proceso de formación para lograr dos medallas de oro para el país.

  5. Infraestructura para laboratorios de acceso remoto

    Directory of Open Access Journals (Sweden)

    López Luro, Francisco

    2009-01-01

    Full Text Available Las tecnologías de Internet permiten el uso de sistemas de software distribuido para el acceso en forma remota a laboratorios físicos y virtuales, para llevar a cabo actividades de investigación experimental a distancia. A partir de los marcos teóricos que definen los estructurantes de los laboratorios, los implementadores de laboratorios de acceso remoto deben diseñar y desarrollar arquitecturas de servicios que permitan un acceso flexible y controlado. Este trabajo define una infraestructura para la implementación de aplicaciones para el acceso remoto a laboratorios físicos y virtuales y para la gestión de los mismos. Se presentan conceptos que permiten extender la modalidad tradicional de conexión con un dispositivo, para llevar adelante actividades prácticas de laboratorio colaborativas y concurrentes.

  6. Leucaena lanceolata S. Watson ssp. lanceolata, ESPECIE FORESTAL CON POTENCIAL PARA SER INTRODUCIDA EN SISTEMAS SILVOPASTORILES

    Directory of Open Access Journals (Sweden)

    María L. Román-Miranda

    2013-01-01

    Full Text Available La utilización de especies forestales en los sistemas de producción agropecuaria contribuye a reducir la presión en los bosques naturales y se pueden incorporar en áreas no arboladas. El objetivo de este estudio fue evaluar la calidad nutritiva, germinación, desarrollo de plántula en vivero y diversidad de usos de Leucaena lanceolata S. Watson ssp. lanceolata. El material comestible y las semillas se colectaron en Tomatlán, Jalisco. Se realizaron análisis bromatológicos, pruebas de escarificación y evaluación de plántula en vivero sobre tres suelos con diferente pH. El experimento se analizó en un diseño completamente al azar con comparación de medias de Tukey (P ≤ 0.05. Además, se hicieron entrevistas a productores, una revisión bibliográfica y consulta de ejemplares en los herbarios para conocer los usos locales y potenciales de la especie. Los resultados indican alto contenido de materia seca (97.40 % y proteína cruda (29.05 %, mayor germinación en los tratamientos térmicos, mejor desarrollo de la plántula en el suelo ligeramente ácido (6.57 y la diversidad de usos incluye leña, forraje y madera, entre otros. Por el alto valor nutritivo y diversidad de usos en el medio rural, L. lanceolata representa una opción viable para utilizarse en sistemas silvopastoriles del trópico seco.

  7. A reliable protocol for the isolation of viable, chondrogenically differentiated human mesenchymal stem cells from high-density pellet cultures.

    Science.gov (United States)

    Ullah, Mujib; Hamouda, Houda; Stich, Stefan; Sittinger, Michael; Ringe, Jochen

    2012-12-01

    Administration of chondrogenically differentiated mesenchymal stem cells (MSC) is discussed as a promising approach for the regenerative treatment of injured or diseased cartilage. The high-density pellet culture is the standard culture for chondrogenic differentiation, but cells in pellets secrete extracellular matrix (ECM) that they become entrapped in. Protocols for cell isolation from pellets often result in cell damage and dedifferentiation towards less differentiated MSC. Therefore, our aim was to develop a reliable protocol for the isolation of viable, chondrogenically differentiated MSC from high-density pellet cultures. Human bone marrow MSC were chondrogenically stimulated with transforming growth factor-β3, and the cartilaginous structure of the pellets was verified by alcian blue staining of cartilage proteoglycans, antibody staining of cartilage collagen type II, and quantitative real-time reverse-transcription polymerase chain reaction of the marker genes COL2A1 and SOX9. Trypsin and collagenases II and P were tested alone or in combination, and for different concentrations and times, to find a protocol for optimized pellet digestion. Whereas trypsin was not able to release viable cells, 90-min digestion with 300 U of collagenase II, 20 U of collagenase P, and 2 mM CaCl2 worked quite well and resulted in about 2.5×10(5) cells/pellet. The protocol was further optimized for the separation of released cells and ECM from each other. Cells were alcian blue and collagen type II positive and expressed COL2A1 and SOX9, verifying a chondrogenic character. However, they had different morphological shapes. The ECM was also uniformly alcian blue and collagen type II positive but showed different organizational and structural forms. To conclude, our protocol allows the reliable isolation of a defined number of viable, chondrogenically differentiated MSC from high-density pellet cultures. Such cells, as well as the ECM components, are of interest as

  8. Pensamiento y construcción de conocimiento histórico, una exigencia para el hacer futuro

    Directory of Open Access Journals (Sweden)

    Hugo Zemelman Merino

    2015-08-01

    Full Text Available Una marcada tendencia en América Latina ha sido la de construir conocimiento sobre la premisa del desarrollo histórico con una dirección más o menos precisa, emancipatoria y valórica. Pero el siglo XX nos legó una historia caprichosa, incierta, fuertemente indeterminada, sin garantía. En este sentido, más que una comprensión pesimista, la historia del siglo XX exige encarnar desafíos sustantivos. Enfrentamos enormes exigencias en lo que se refiere a desarrollar nuestra capacidad de pensar como latinoamericanos; y esto, atañe a la formación de sujetos capaces de ver y pensar realidades inéditas y viables, para lo cual es imprescindible replantear las formas de construir conocimiento; entendiendo que éste debería ser de naturaleza histórica más que teórica de cara a ampliar los límites de lo posible, en perspectiva de futuro. A marked trend in Latin America has been the one of constructing knowledge on the premise of the historical development with a more or less precise, emancipatory, and valued direction. But the twentieth century gave us a capricious, uncertain, highly indeterminate, and unsecured history. In this sense, rather than a pessimistic understanding, the history of the twentieth century requires embodying substantive challenges. We face enormous demands in regards to developing our ability to think as Latin Americans; and this relates to the formation of subjects able to see and to think new and viable realities, which is essential to rethink the ways to construct knowledge; understanding that this should be historical in nature rather than theoretical in the face to expand limits as possible, in perspective for the future.

  9. Viable cold-tolerant iron-reducing microorganisms in geographically diverse subglacial environments

    Science.gov (United States)

    Nixon, Sophie L.; Telling, Jon P.; Wadham, Jemma L.; Cockell, Charles S.

    2017-03-01

    Subglacial environments are known to harbour metabolically diverse microbial communities. These microbial communities drive chemical weathering of underlying bedrock and influence the geochemistry of glacial meltwater. Despite its importance in weathering reactions, the microbial cycling of iron in subglacial environments, in particular the role of microbial iron reduction, is poorly understood. In this study we address the prevalence of viable iron-reducing microorganisms in subglacial sediments from five geographically isolated glaciers. Iron-reducing enrichment cultures were established with sediment from beneath Engabreen (Norway), Finsterwalderbreen (Svalbard), Leverett and Russell glaciers (Greenland), and Lower Wright Glacier (Antarctica). Rates of iron reduction were higher at 4 °C compared with 15 °C in all but one duplicated second-generation enrichment culture, indicative of cold-tolerant and perhaps cold-adapted iron reducers. Analysis of bacterial 16S rRNA genes indicates Desulfosporosinus were the dominant iron-reducing microorganisms in low-temperature Engabreen, Finsterwalderbreen and Lower Wright Glacier enrichments, and Geobacter dominated in Russell and Leverett enrichments. Results from this study suggest microbial iron reduction is widespread in subglacial environments and may have important implications for global biogeochemical iron cycling and export to marine ecosystems.

  10. Diseño de imágenes para ciegos, material didáctico para niños con discapacidad visual.

    OpenAIRE

    FUENTES NIEVES, FABIOLA MIREYA

    2014-01-01

    El objetivo de este proyecto es contribuir investigación para la creación, la aplicación, la transformación del quehacer profesional del diseño gráfico en el conocimiento y las habilidades, en la formación de profesionales en el área del diseño para material didáctico para esta disciplina, por lo cual es necesario cambiar respuestas para lo local y/o para la globalización. En la historia del diseño, se plantea, proyectar para la globalización, para la humanidad. Ahora el planteamiento de r...

  11. El efecto de la disponibilidad de agua durante el crecimiento de Lycopersicon chilense sobre la capacidad de sus semillas para germinar a distintas temperaturas y concentraciones de manitol y NaCl Effect of water availability during the growth of Lycopersicon chilense on the capacity of their seeds to germinate at different temperatures and concentrations of manitol and NaCl

    Directory of Open Access Journals (Sweden)

    CARLOS MALDONADO

    2002-12-01

    Full Text Available El número de semillas producidas por una planta puede estar limitada por factores ambientales adversos, los cuales también pueden afectar el desarrollo, originando semillas no viables. En la Primera Región de Chile hemos observado que Lycopersicon chilense, una especie de tomate endémica del Desierto de Atacama, presenta una alta producción de frutos y de semillas, sin embargo se observa una escasa regeneración de nuevos individuos. Se plantea que esto se debe a dos factores: (a las plantas madres, producto de las condiciones adversas donde crecen desarrollan semillas no viables o (b las semillas son viables pero las condiciones ambientales son desfavorables para la germinación. Se estudió la capacidad germinativa de semillas de Lycopersicon chilense sometidas a distintas temperaturas y concentraciones de manitol y NaCl, provenientes de plantas madres que crecieron con buen abastecimiento hídrico (+H2O y de otro grupo que creció con un abastecimiento hídrico deficiente (-H2O. El nivel de riego tuvo un efecto sobre el número de semillas germinadas y sobre el tiempo que necesitan las semillas para germinar frente a distintas temperaturas y diferentes potenciales hídricos. Las semillas de plantas -H2O fueron capaces de germinar a potenciales hídricos más negativos y en menor tiempo que las semillas provenientes de plantas +H2O en el caso de manitol. Una relación inversa ocurrió respecto a NaCl, en que un mayor porcentaje de germinación y un menor tiempo de germinación se presentó en las semillas provenientes de plantas +H2O. La germinación de L. chilense disminuye progresivamente a potenciales hídricos menores de -0,5 MPa siendo esta disminución más notoria con NaCl, en ambos casos se observó un retraso en la germinación. El óptimo de temperatura para la germinación se encontró entre los 15 y los 25 °C. Lycopersicon chilense no germinó a temperaturas menores de 8 °C y mayores de 35 °C. Los datos mostraron que las

  12. Systems thinking benefits in supply change management:an illustration of the viable systems model in a supply chain

    OpenAIRE

    Chronéer, Diana; Mirijamdotter, Anita

    2009-01-01

    Existing product development models are solely an organisational matter. They do not take into consideration the whole supply chain and its different actors. We investigate in this paper how Supply Chain Management (SCM) and Viable System Model (VSM) can support and create an effective use of information in product development and hence identify critical linkages in the supply chain. The aim is to introduce VSM as a framework that enable an analysis of company's supply chains and visualize vi...

  13. An economically viable alternative to coastal discharge of produced water

    International Nuclear Information System (INIS)

    D'Unger, C.V.; Carr, R.S.; Chapman, D.C.

    1993-01-01

    The discharge of produced waters to coastal estuaries has been common practice on the Texas coast for many years as these discharges are currently exempt from NPDES permitting. A study of the active produced water discharges in Nueces Bay, Texas revealed that all eight effluents were highly toxic as determined by the sea urchin (Arbacia punctulata) fertilization and embryological development assays. An alternative to discharging produced water into coastal estuaries is the use of disposal wells. Inactive wells can be converted to produced water disposal wells. Production records for the Nueces Bay, Texas area reveal that 52% of the gas wells produce less than 100 mcf/d and 50% of the oil wells produce less than 10 b/d. Using conservative estimates, the cost of converting an inactive well to a disposal well was calculated to be $31,500 which could be paid out by a gas well producing as little as 100 mcf/d in 26 months using only 50% of the well's profit. Combining multiple leases to a single disposal well would reduce proportionately the cost to each operation. This study has demonstrated that economically viable disposal options could be achieved in the Nueces Bay area through the imaginative and cooperative formation of produced water disposal ventures. This same model could be applied to produced water discharges in other coastal areas

  14. Heterogeneity, politics of ethnicity, and multiculturalism What is a viable framework for Indonesia?

    Directory of Open Access Journals (Sweden)

    Thung Ju Lan

    2011-10-01

    Full Text Available Indonesia is a plural society that consists of several hundred ethnic and sub-ethnic groups. One of its generic characteristics is heterogeneity. In the last ten years after the implementation of regional autonomy, we have witnessed the emergence of strong ethnic and religiously flavoured local identity politics in various places in Indonesia that created open and vicious conflicts. This periodical violence exploded especially during the election of district and provincial heads. The intimate relation multiculturalism, with the actual political praxis of everyday life as an alternative to the existing paradigm of the “homogenization” of nationhood, has not been discussed. I believe it is time to discuss the strategic junctures between heterogeneity, politics of ethnicity (and religion and multiculturalism as well as their possible realization at the local and national levels in order to find a viable framework for a future Indonesia.

  15. Calculation of attenuation by rain using the DAH model and diameter of antennas for the Ka Band in Mexico; Calculo de atenuacion por lluvia usando el modelo DAH y diametro de antena para Banda Ka en Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Landeros-Ayala, S.; Neri-Vela, R; Cruz-Sanchez, H.; Hernandez-Bautista, H. [Universidad Nacional Autonoma de Mexico, Mexico, D.F. (Mexico)

    2002-03-01

    In the last years, the peak in the demand of satellite communication service has caused the saturation in the use of the frequencies corresponding to the band, Cand Ku. Due to this, the engineers have looked for viable alternatives, in order to satisfy the current requisition, as well as the future demand, for which a considerable increment is expected. One of these alternatives is the use of the Ka Band (20Hz/30Hz), that is why the importance of studying the propagation effects that are experienced at these frequencies, especially the attenuation effect by rain, as in this case, where it is significant. The present article has the purpose to describe the use of the Modelo DAH (whose authors are Asoka Dissanayake, Jeremy Allnutt and Fatim Haidara), mixed with the global maps of distribution of rain by Crane, for the calculation of the attenuation by rain in satellite communication systems operated in the Ka Band. Besides, antenna diameters for the systems of communications in Ka Band in different locations of the Mexican Republic, using for it the attenuation margins for rain obtained through the Modelo DAH, and using as references the characteristics of the ANIK F2 satellite and a terrestrial station VSAT, are proposed. [Spanish] En los ultimos anos, el auge en la demanda de servicios de comunicacion por satelite ha provocado la saturacion en los uso de la frecuencia correspondientes a las bandas C y Ku. Debido a esta razon, se han buscado alternativas viables para poder satisfacer la demanda actual, asi como la demanda futura, para la cual se espera un incremento considerable. Una de estas alternativas es el uso de Banda Ka (20Hz/30Hz), de ahi la importancia del estudio sobre los efectos de programacion que se experimentan a esta frecuencia, en especial, el efecto de atencion por lluvias, ya que sen este caso resulta ser significativa. El presente articulo tiene como finalidad describir el uso del Modelo DAH (cuyos autores son Asoka Dissanayake, Jeremy Allnutt y

  16. The para-petroleum industry

    International Nuclear Information System (INIS)

    2002-07-01

    The para-petroleum industry includes societies that realize studies and installations for the deposits exploitation. This document presents the situation of the para-petroleum industry in 2001, the world investment growth of the activity, the french societies financial results and an inventory of the main operations in the amalgamation domain. (A.L.B.)

  17. Viable adhered Staphylococcus aureus highly reduced on novel antimicrobial sutures using chlorhexidine and octenidine to avoid surgical site infection (SSI)

    Science.gov (United States)

    Schneider, Jochen; Harrasser, Norbert; Tübel, Jutta; Mühlhofer, Heinrich; Pförringer, Dominik; von Deimling, Constantin; Foehr, Peter; Kiefel, Barbara; Krämer, Christina; Stemberger, Axel; Schieker, Matthias

    2018-01-01

    Background Surgical sutures can promote migration of bacteria and thus start infections. Antiseptic coating of sutures may inhibit proliferation of adhered bacteria and avoid such complications. Objectives This study investigated the inhibition of viable adhering bacteria on novel antimicrobially coated surgical sutures using chlorhexidine or octenidine, a critical factor for proliferation at the onset of local infections. The medical need, a rapid eradication of bacteria in wounds, can be fulfilled by a high antimicrobial efficacy during the first days after wound closure. Methods As a pretesting on antibacterial efficacy against relevant bacterial pathogens a zone of inhibition assay was conducted with middle ranged concentrated suture coatings (22 μg/cm). For further investigation of adhering bacteria in detail the most clinically relevant Staphylococcus aureus (ATCC®49230™) was used. Absorbable braided sutures were coated with chlorhexidine-laurate, chlorhexidine-palmitate, octenidine-laurate, and octenidine-palmitate. Each coating type resulted in 11, 22, or 33 μg/cm drug content on sutures. Scanning electron microscopy (SEM) was performed once to inspect the coating quality and twice to investigate if bacteria have colonized on sutures. Adhesion experiments were assessed by exposing coated sutures to S. aureus suspensions for 3 h at 37°C. Subsequently, sutures were sonicated and the number of viable bacteria released from the suture surface was determined. Furthermore, the number of viable planktonic bacteria was measured in suspensions containing antimicrobial sutures. Commercially available sutures without drugs (Vicryl®, PGA Resorba®, and Gunze PGA), as well as triclosan-containing Vicryl® Plus were used as control groups. Results Zone of inhibition assay documented a multispecies efficacy of novel coated sutures against tested bacterial strains, comparable to most relevant S. aureus over 48 hours. SEM pictures demonstrated uniform layers on

  18. Detection of viable and viable nonculturable Vibrio cholerae O1 through cultures and immunofluorescence in the Tucumán rivers, Argentina Detecção de Vibrio cholerae O1 viável e viável não cultivável, através de técnicas de cultivo e imunofluorescência nos rios de Tucumán, Argentina

    Directory of Open Access Journals (Sweden)

    Olga Aulet

    2007-08-01

    Full Text Available Vibrio cholerae has been sporadically isolated from rivers in Tucumán, Argentina, since the outbreak in 1991. The aim of this study was to determine the environmental reservoir of the bacterium in these rivers, assessing the presence of Vibrio cholerae non-O1 and O1 (the latter both in its viable culturable and non culturable state and its relationship to environmental physicochemical variables. 18 water samplings were collected in the Salí River (in Canal Norte and Banda and the Lules River between 2003 and 2005. Physical-chemical measurements (pH, water temperature, electrical conductivity and dissolved oxygen were examined. Vibrio cholerae was investigated with conventional culture methods and with Direct Immunofluorescence (DFA-VNC in order to detect viable non culturable organisms. All isolated microorganisms corresponded to Vibrio cholerae non-O1 and non-O139 (Lules 26%, Canal Norte 33% and Banda 41%. The majority was found during spring and summer and correlated with temperature and pH. Non culturable Vibrio cholerae O1 was detected year round in 38 of the 54 water samples analyzed. Application of the Pearson correlation coefficient revealed that there was no relationship between positive immunofluorescence results and environmental physicochemical parameters. Genes coding for somatic antigen O1 were confirmed in all DFA-VNC-positive samples, whereas the virulence-associated ctxA and tcpA genes were confirmed in 24 samples.Vibrio cholerae tem sido isolado esporadicamente nos rios da Província de Tucumán, Argentina, desde outubro de 1991. O objetivo deste estudo foi localizar os reservatórios nestes rios, identificar a presença de Vibrio cholerae O1 (em estado cultivável e não cultivável e relacionar a presença desta bactéria com as variações físico-químicos da água. Foram coletadas dezoito amostras de água do rio Salí (nas localidades de Canal Norte e Banda e do rio Lules, entre 2003 e 2005. Estas foram submetidas a an

  19. Alterações fisiológicas da morte encefálica em potenciais doadores de órgãos e tecidos para transplantes Los cambios fisiológicos de la muerte cerebral en potenciales donadores de órganos y tejidos para trasplante Physiological changes of brain death in potential donors of organs and tissues for transplantation

    Directory of Open Access Journals (Sweden)

    Sarah Gabriel Freire

    2012-12-01

    órnea (3,1%. Se cree que el conocimiento de estos cambios permite al equipo de atención de la salud dirigir sus acciones al potencial donador de acuerdo a sus necesidades y así mantener los órganos/tejidos viables para el trasplante.The objective was to describe the physiologic changes of brain death in potential donors of organs and tissues for transplantation. Exploratory descriptive study with prospective data and quantitative approach carried out in emergency and intensive care units hospital adult, in the period from April to October 2011. The population consisted of 32 potential donors of organs and tissues for transplantation. After approval of Ethics Committee, data were collected, tabulated and analyzed by descriptive statistics by SPSS 15.0 software and presented in tables. Physiological changes were: hypotension (100%, hypothermia (75%, hypernatremia (62,5%, diabetes insipidus (37,5%, hyperglycemia (32,3%, infection (25,0%, hypertension (9,4% and corneal ulcer (3,1%. It was found that knowledge of these changes allows the team of health care to direct the potential donors according to their needs and thus keep the organ/tissue viable for transplant.

  20. Detection of viable Helicobacter pylori inside free-living amoebae in wastewater and drinking water samples from Eastern Spain.

    Science.gov (United States)

    Moreno-Mesonero, Laura; Moreno, Yolanda; Alonso, José Luis; Ferrús, M Antonia

    2017-10-01

    Helicobacter pylori is one of the most concerning emerging waterborne pathogens. It has been suggested that it could survive in water inside free-living amoebae (FLA), but nobody has studied this relationship in the environment yet. Thus, we aimed to detect viable H. pylori cells from inside FLA in water samples. Sixty-nine wastewater and 31 drinking water samples were collected. FLA were purified and identified by PCR and sequencing. For exclusively detecting H. pylori inside FLA, samples were exposed to sodium hypochlorite and assayed by specific PMA-qPCR, DVC-FISH and culture. FLA were detected in 38.7% of drinking water and 79.7% of wastewater samples, even after disinfection. In wastewater, Acanthamoeba spp. and members of the family Vahlkampfiidae were identified. In drinking water, Acanthamoeba spp. and Echinamoeba and/or Vermamoeba were present. In 39 (58.2%) FLA-positive samples, H. pylori was detected by PMA-qPCR. After DVC-FISH, 21 (31.3%) samples harboured viable H. pylori internalized cells. H. pylori was cultured from 10 wastewater samples. To our knowledge, this is the first report that demonstrates that H. pylori can survive inside FLA in drinking water and wastewater, strongly supporting the hypothesis that FLA could play an important role in the transmission of H. pylori to humans. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

  1. Contrast-Enhanced Ultrasound Improves the Pathological Outcomes of US-Guided Core Needle Biopsy That Targets the Viable Area of Anterior Mediastinal Masses

    Directory of Open Access Journals (Sweden)

    Jian-hua Zhou

    2018-01-01

    Full Text Available Based on the option that ultrasound-guided core needle biopsy (US-CNB of the enhanced portion of anterior mediastinal masses (AMMs identified by contrast-enhanced ultrasound (CEUS would harvest viable tissue and benefit the histological diagnoses, a retrospective study was performed to elucidate the correlation between the prebiopsy CEUS and diagnostic yield of AMMs and found that CEUS potentially improved the diagnostic yield of AMMs compared with conventional US with a significant increase in the cellularity of samples. Furthermore, the marginal blood flow signals and absence of necrosis can predict the diagnostic yield of AMM. It was concluded that US-CNB of the viable part of AMMs, as verified by CEUS, was able to harvest sufficient tissue with more cellularity that could be used for ancillary studies and improve the diagnostic yield. And CEUS was recommended to those patients with AMMs undergoing repeated US-CNB, with the absence of marginal blood signals or presence of necrosis.

  2. Adesao ao guia alimentar para populacao brasileira

    Directory of Open Access Journals (Sweden)

    Eliseu Verly Junior

    2013-12-01

    Full Text Available OBJETIVO : Analisar a adesão ao Guia Alimentar para População Brasileira. MÉTODOS : Amostra composta por participantes do Inquérito de Saúde de São Paulo (n = 1.661 que preencheram dois recordatórios de 24 horas. Foi utilizado modelo bivariado de efeito misto para a razão entre o consumo de energia do grupo de alimentos e o consumo calórico total. A razão estimada foi utilizada para calcular o percentual de indivíduos com consumo abaixo ou acima da recomendação. RESULTADOS : Pelo menos 80,0% da população consome abaixo do recomendado para: leite e derivados; frutas e sucos de frutas; e cereais, tubérculos e raízes; aproximadamente 60,0% para legumes e verduras; 30,0% para feijões; e 8,0% para carnes e ovos. Adolescentes apresentaram a maior inadequação para legumes e verduras (90,0%, e o estrato de maior renda foi associado à menor inadequação para óleos, gorduras e sementes oleaginosas (57,0%. CONCLUSÕES : Foi observado consumo inadequado dos grupos de alimentos relacionados com aumento do risco de doenças crônicas.

  3. Viable Cancer Cells in the Remnant Stomach are a Potential Source of Peritoneal Metastasis after Curative Distal Gastrectomy for Gastric Cancer.

    Science.gov (United States)

    Murata, Satoshi; Yamamoto, Hiroshi; Yamaguchi, Tsuyoshi; Kaida, Sachiko; Ishida, Mitsuaki; Kodama, Hirokazu; Takebayashi, Katsushi; Shimizu, Tomoharu; Miyake, Toru; Tani, Tohru; Kushima, Ryoji; Tani, Masaji

    2016-09-01

    The mechanisms underlying peritoneal metastasis (PM) after curative gastrectomy for gastric cancer (GC) are not well elucidated. This study assessed whether viable cancer cells, including cancer stemlike cells (CSCs), were present in the remnant stomach immediately before gastrointestinal (GI) tract reconstruction because these could be a source of PM after gastrectomy. Saline fluid used for remnant stomach lumen irrigation before GI reconstruction was prospectively collected from 142 consecutive patients undergoing distal gastrectomy for GC and cytologically examined. Proliferative activity (Ki67 staining) and stemness (expression of the CSC surface markers CD44s or CD44v6) were evaluated in detected cancer cells. Viable cancer cells were detected in 33 (23.2 %) of the 142 remnant stomachs. These cells formed clusters and stained positively for Ki67, indicating proliferation. Cancer cells in remnant stomachs and surface cancer cells in primary GCs from 10 (30.3 %) of these 33 cases also stained positively for CD44s or CD44v6. In a multiple logistic regression analysis, advanced cancer (odds ratio [OR], 4.65; 95 % confidence interval [CI], 1.32-16.4; P = 0.017), tumor size of 40 mm or larger (OR, 3.78; 95 % CI, 1.12-12.8; P = 0.033), and histologic differentiation (OR, 3.10; 95 % CI, 1.30-7.40; P = 0.011) were associated independently with the presence of cancer cells in the remnant stomach. Viable, proliferative, and clustered cancer cells, including CSCs, were found in remnant gastric lumens immediately before GI reconstruction, indicating a possible cellular source of PM after curative gastrectomy for GC. Dissemination of gastric contents into the peritoneal cavity should be avoided during GI reconstruction.

  4. Algoritmos para problemas de empacotamento

    OpenAIRE

    Eduardo Candido Xavier

    2006-01-01

    Resumo: Neste trabalho estudamos diversos problemas de empacotamento considerados NP-difíceis. Assumindo a hipótese de que P ? NP, sabemos que não existem algoritmos eficientes (complexidade de tempo polinomial) exatos para resolver tais problemas. Uma das abordagens consideradas para tratar tais problemas é a de algoritmos de aproximação, que são algoritmos eficientes e que geram soluções com garantia de qualidade. Neste trabalho apresentamos alguns algoritmos aproximados para problemas de e...

  5. Correlations between cyanobacterial density and bacterial transformation to the viable but nonculturable (VBNC) state in four freshwater water bodies.

    Science.gov (United States)

    Chen, Huirong; Shen, Ju; Pan, Gaoshan; Liu, Jing; Li, Jiancheng; Hu, Zhangli

    2015-10-01

    Nutrient concentrations, phytoplankton density and community composition, and the viable but nonculturable (VBNC) state of heterotrophic bacteria were investigated in three connected reservoirs and a small isolated lake in South China to study the relationship between biotic and abiotic factors and the VBNC state in bacteria. Nutrient concentrations in the reservoirs increased in the direction of water flow, whereas Wenshan Lake was more eutrophic. Cyanobacterial blooms occurred in all four water bodies, with differing seasonal trends and dominant species. In Xili and Tiegang Reservoirs, the VBNC ratio (percent of VBNC state bacteria over total viable bacteria) was high for most of the year and negatively correlated with cyanobacterial density. Laboratory co-culture experiments were performed with four heterotrophic bacterial species isolated from Wenshan Lake (Escherichia coli, Klebsiella peneumoniae, Bacillus megaterium and Bacillus cereus) and the dominant cyanobacterial species (Microcystis aeruginosa). For the first three bacterial species, the presence of M. aeruginosa induced the VBNC state and the VBNC ratio was positively correlated with M. aeruginosa density. However, B. cereus inhibited M. aeruginosa growth. These results demonstrate that cyanobacteria could potentially regulate the transformation to the VBNC state of waterborne bacteria, and suggest a role for bacteria in cyanobacterial bloom initiation and termination.

  6. Metodología para el diseño de un biorreactor secuencial - Methodology for the Design of a Sequential Bioreactor

    Directory of Open Access Journals (Sweden)

    Pedro Nel Martínez Henao

    2017-06-01

    Full Text Available El artículo presenta la metodología de diseño de un Biorreactor secuencial SBR requerido en el proceso de tratamiento de aguas residuales. El proceso aeróbico empleado ayuda a la oxigenación del agua para que las bacterias realicen la degradación y procesamiento de material particulado y generen los lodos activados, que es un material que puede ser reutilizado para compostaje en procesos de agricultura orgánica. La medición de PH en el agua ayuda al control de bacterias que se hace mediante el suministro de aire y/o levadura en el agua. El proyecto es desarrollado con la participación de estudiantes de los programas de Ingeniería Ambiental e Ingeniería Industrial, adscritos al semillero de investigación SIDI en el marco del proyecto de iniciación científica PIC-ING-2252, financiada por la Universidad Militar Nueva Granada [UMNG]. La metodología de diseño a emplearse en el diseño del SBR es la expuesta por Arzola [1] y Martínez [2], abordando inicialmente la matriz de despliegue de función de calidad (QfD, en el que se registran inicialmente los requerimientos del cliente para poderlos traducir a las especificaciones técnicas de ingeniería: posteriormente se desarrolla el diseño conceptual (caja gris, donde se plantean alternativas que dan cumplimiento a los requerimientos y a las especificaciones técnicas de ingeniería. Seleccionada la alternativa más viable, se procede al modelamiento, cálculo y selección de componentes a ser utilizados en el concepto seleccionado como alternativa de desarrollo. Finalmente, se realiza la ingeniería de detalle mediante modelamiento CAD.

  7. Individualized teaching programming for a virtual learning environment: development of content concerning nursing records Programación de enseñanza individualizada para ambiente virtual de aprendizaje: elaboración del contenido registro de enfermería Programação de ensino individualizado para ambiente virtual de aprendizagem: elaboração do conteúdo registro de enfermagem

    Directory of Open Access Journals (Sweden)

    Patrícia de Carvalho Nagliate

    2013-02-01

    Full Text Available OBJECTIVE: Describe the planning of contents on nursing records for use in a virtual learning environment, based on Individualized Teaching Programming, a didactic resource that uses basic principles of behavioral analysis. METHOD: Final objectives were specified, after defining the intermediary components to achieve each final objective, as well as the preliminary requirements for each intermediary component. At the end of this process, teaching activities were planned and organized in steps the students need to develop. RESULTS: By breaking up the contents into behaviors, seven action categories emerged: impartiality, organization, honesty, objectiveness, coherence, readability and discernment. CONCLUSION: the use of Individualized Teaching Programming as a didactic resource to plan contents on nursing records is feasible to identify the units and modules for the development of a course in a virtual learning environment for nursing professionals.OBJETIVO: Describir la planificación de contenido sobre registro de enfermería, para utilización en ambiente virtual de aprendizaje, con base en la Programación de Enseñanza Individualizada, un recurso didáctico que utiliza principios básicos del análisis del comportamiento. MÉTODO: fueron especificados objetivos finales, definidos los componentes intermediarios para alcanzar cada objetivo terminal, así como requisitos antecedentes para cada componente intermediario. Al final de ese proceso, fueron planeadas y organizadas las actividades de enseñanza en pasos que serán desarrollados por los aprendices. RESULTADOS: Al desmembrar el contenido en comportamientos, siete categorías de acciones emergieron: imparcialidad, organización, honestidad, objetividad, coherencia, legibilidad y discernimiento. CONCLUSIÓN: la utilización de la Programación de Enseñanza Individualizada como recurso didáctico para la planificación de contenido sobre registro de enfermería se mostró viable para el

  8. Alternativas orgánicas para disminuir la aplicación de nitrógeno en nogal pecanero

    Directory of Open Access Journals (Sweden)

    Juan Manuel Soto Parra

    2016-01-01

    ; esta dosis podría asegurar la calidad de la producción actual y proveer al árbol de nutrientes para la siguiente producción. Discusión o Conclusión: Se logró disminuir la aplicación de nitrógeno hasta 27 kg ha-1 para una producción de 2.9 t ha-1; no obstante, este nutriente por sí solo no permitió incrementar la fertilidad del suelo y mejorar la calidad de la nuez, por lo que es necesario la adición de enmiendas orgánicas y micorrizas. Con esta información es posible concluir que la fertilización nitregenada inorgánica complementada con enmiendas orgánicas y micorrizas, pudiera ser una alternativa viable para mejorar la fertilidad del suelo y la producción en nogal pecanero.

  9. Viable tumor in salvage neck dissections in head and neck cancer : Relation with initial treatment, change of lymph node size and human papillomavirus

    NARCIS (Netherlands)

    van den Bovenkamp, Karlijn; Dorgelo, Bart; Noordhuis, Maartje G; van der Laan, Bernard F A M; van der Vegt, Bert; Bijl, Hendrik P; Roodenburg, Jan L; van Dijk, Boukje A C; Oosting, Sjoukje F; Schuuring, Ed M D; Langendijk, Johannes A; Halmos, Gyorgy B; Plaat, Boudewijn E C

    Objectives: To identify predictive factors for the presence of viable tumor and outcome in head and neck cancer patients who undergo therapeutic salvage neck dissections. Materials and Methods: Retrospective analysis of 76 salvage neck dissections after radiotherapy alone (n = 22), radiotherapy in

  10. Construcción de un indicador para medir competencias básicas para la vida

    Directory of Open Access Journals (Sweden)

    Andrea Collado Chaves

    2006-01-01

    Full Text Available La necesidad de construir un indicador para cuantificar el nivel de Competencias Básicas para la Vida (CBV, nace con la evaluación los resultados de un proyecto dirigido a población joven. El propósito de ese proyecto es construir capital humano y empleabilidad en hombres y mujeres con edades entre 13 y 18 años, desertores o en riesgo de desertar del sistema educativo, sin empleo formal y que viven en ambientes de pobreza y exclusión. Una de las dimensiones de empleabilidad son las Competencias Básicas para la Vida. La dimensión de competencias básicas para la vida se refiere a aspectos de personalidad asociados con el desempeño del individuo en sus relaciones consigo mismo y con los demás. Los datos provienen de una investigación cuyo propósito consiste en evaluar los resultados de una intervención en población joven de comunidades pobres y excluidas. La construcción del indicador uso técnicas psicométricas para la validación de las distintas subdimensiones que pertenecen al concepto de competencias básicas para la vida. Según el marco conceptual este indicador está compuesto por seis dimensiones, la validación empírica hecha en este artículo incluye solamente cuatro de ellas, todas de carácter actitudinal. El indicador posee algunas ventajas en términos de la capacidad de discriminación debido a que su distribución tiende a ser normal. Sin embargo se ve afectaba por valores extremos a la izquierda del promedio y valores modales a una desviación estándar (positiva del promedio. Estos resultados serán útiles para discusiones futuras sobre los puntos de corte (baremos, el comportamiento del indicador cuando se correlaciona con deseabilidad social y la integración de la dimensión de Competencias Básicas para la Vida en el indicador de empleabilidad.

  11. Quantification des cellules viables de P. phosphoreum dans les pavés de saumon cru par PCR temps reel

    DEFF Research Database (Denmark)

    Macé, Sabine; Mamlouk, Kelthoum; Chipchakova, Stoyka

    ’altération. Par conséquent, nous avons développé une méthode de PCR temps réel spécifique combinée avec une étape de traitement au PMA pour quantifier les cellules viable de P.phosphoreum dans le saumon cru conditionné sous atmosphère modifiée. Les amorces spécifiques ont été dessinées pour amplifier un fragment...

  12. The viable but non-culturable state in pathogenic Escherichia coli: A general review

    Directory of Open Access Journals (Sweden)

    Jennifer A. Pienaar

    2016-05-01

    Objectives: This review discusses various general aspects of the VBNC state, the mechanisms and possible public health impact of indicator and pathogenic E. coli entering into the VBNC state. Method: A literature review was conducted to ascertain the possibleimpact of E. coli entering into the VBNC state. Results: Escherichia coli enter into the VBNC state by means of several induction mechanisms. Various authors have found that E. coli can be resuscitated post-VBNC. Certain strains of pathogenic E. coli are still able to produce toxins in the VBNC state, whilst others are avirulent during the VBNC state but are able to regain virulence after resuscitation. Conclusion: Pathogenic and indicator E. coli entering into the VBNC state could have an adverse effect on public health if conventional detection methods are used, where the number of viable cells could be underestimated and the VBNC cells still produce toxins or could, at anytime, be resuscitated and become virulent again.

  13. Propuesta metodológica para hacer un diagnóstico de la Educación para el Desarrollo

    Directory of Open Access Journals (Sweden)

    Francisco Javier Gómez González

    2012-01-01

    Full Text Available El presente artículo quiere dar a conocer el diseño metodológico para la elaboración de un diagnóstico de situación de la educación para el desarrollo en Castilla y León, que se ha ideado desde el Observatorio de Cooperación Internacional de la Universidad de Valladolid con el fin de que el conocimiento sobre la situación actual de la educación para el desarrollo en Castilla y León sirva de base para la formulación de una futura estrategia de acción en educación para el desarrollo en la Comunidad.

  14. Requisitos de clientes para o desenvolvimento de um implemento a fim de fechar taipas abertas para drenagem

    Directory of Open Access Journals (Sweden)

    Ulisses Giacomini Frantz

    2015-04-01

    Full Text Available O arroz irrigado é cultivado na maior parte do RS com aplainamento da superfície do solo em desnível e sistema de cultivo mínimo. Com a semeadura em solo seco, há necessidade de conformar drenos, que cortam transversalmente as taipas, na área de cultivo. Para o estabelecimento da lâmina de água definitiva, essas taipas abertas para drenagem devem ser fechadas e, para essa finalidade, não existe um equipamento específico que execute tal atividade com devida agilidade e qualidade, sendo necessário o desenvolvimento de um implemento com este fim. Ainda, para o processo de desenvolvimento de máquinas agrícolas, faz-se necessário o levantamento de uma série de informações. Na fase de projetação, essas informações são necessárias para o estabelecimento das necessidades dos clientes/usuários e dos requisitos dos clientes, para posteriormente serem transformadas em requisitos de projeto e estabelecidas as especificações de projeto. Desse modo, este trabalho objetivou determinar os requisitos dos clientes, a partir das suas necessidades, para o projeto de um implemento com a função de fechamento de taipas abertas para drenagem no arroz irrigado. As informações foram levantadas a partir de pesquisas bibliográficas e aplicação de questionários a futuros clientes/usuários. Como resultados, foram identificadas as necessidades dos clientes, assim como 25 requisitos de clientes

  15. Hiperacumulación de metales : ¿una ventaja para la planta y para el hombre?

    OpenAIRE

    Llugany i Ollé, Mercè; Tolrà Pérez, Roser; Poschenrieder, Charlotte; Barceló Coll, Juan

    2007-01-01

    La hiperacumulación es la capacidad que tienen algunas plantas para concentrar metales en sus tejidos a niveles muy por encima de los normales sin presentar síntomas de toxicidad. Estas plantas hiperacumuladoras han desarrollado mecanismos internos de tolerancia a la toxicidad por metales. Esta peculiaridad las hace útiles para el hombre como herramienta en las nuevas tecnologías de fitoremediación. Sin embargo, ¿cual es el beneficio de esta hiperacumulación de metales para la propia planta? ...

  16. Hiperacumulación de metales: ¿una ventaja para la planta y para el hombre?

    OpenAIRE

    M. Llugany; R. Tolrà; C. Poschnrieder; J. Barceló

    2007-01-01

    La hiperacumulación es la capacidad que tienen algunasplantas para concentrar metales en sus tejidos a niveles muy por encima de los normales sin presentar síntomas de toxicidad. Estas plantas hiperacumuladoras han desarrollado mecanismos internos de tolerancia a la toxicidad por metales. Esta peculiaridad las hace útiles para el hombre como herramienta en las nuevas tecnologías de fitoremediación. Sin embargo, ¿cual es el beneficio de esta hiperacumulación de metales para la propia planta? S...

  17. ¿Para que la historia?

    OpenAIRE

    Jaime Jaramillo Uribe

    1998-01-01

    Para qué sirve la historia?. ¿Puede el conocimiento del pasado darnos pautas para comprender el presente? Sobre este, como sobre tantos problemas teóricos de la historia, se han realizado largos e inacabados debates entre historiadores y filósofos. En este ensayo se acepta como eficaz la hipótesis de que conocer el origen y el desarrollo de un proceso es buen fundamento para explicarse una situación final. En el caso de la historia colombiana, se parte de la base de que al producirse la indep...

  18. R CORONAE BOREALIS STARS ARE VIABLE FACTORIES OF PRE-SOLAR GRAINS

    International Nuclear Information System (INIS)

    Karakas, Amanda I.; Ruiter, Ashley J.; Hampel, Melanie

    2015-01-01

    We present a new theoretical estimate for the birthrate of R Coronae Borealis (RCB) stars that is in agreement with recent observational data. We find the current Galactic birthrate of RCB stars to be ≈25% of the Galactic rate of Type Ia supernovae, assuming that RCB stars are formed through the merger of carbon–oxygen and helium-rich white dwarfs. Our new RCB birthrate (1.8 × 10 −3 yr −1 ) is a factor of 10 lower than previous theoretical estimates. This results in roughly 180–540 RCB stars in the Galaxy, depending on the RCB lifetime. From the theoretical and observational estimates, we calculate the total dust production from RCB stars and compare this rate to dust production from novae and born-again asymptotic giant branch (AGB) stars. We find that the amount of dust produced by RCB stars is comparable to the amounts produced by novae or born-again post-AGB stars, indicating that these merger objects are a viable source of carbonaceous pre-solar grains in the Galaxy. There are graphite grains with carbon and oxygen isotopic ratios consistent with the observed composition of RCB stars, adding weight to the suggestion that these rare objects are a source of stardust grains

  19. Existence of both culturable and viable but non culturable (VNC) E. coli populations with distinct settling velocities in karst aquifer

    Science.gov (United States)

    Petit, F.; Ratajczak, M.; Massei, N.; Lafite, R.; Clermont, O.; Denamur, E.; Berthe, T.

    2012-12-01

    The karst aquifers are particularly vulnerable to contamination by faecal pathogens mainly during rainfall event. In groundwater, the fate of E. coli is dependent on their ability to overcome environmental stresses and on their association with particles. Moreover, some strains can survive leading to the emergence of a sub-population of E. coli which failed to grow on laboratory media, while they were still alive thus designated as viable but non culturable (VNC). The aim of this study was to investigate (i) the structure of culturable E. coli population based on the survival ability, the distribution in four main phylo-groups (A, B1, B2, D) and the phenotypic characteristics; and, (ii) the fate of culturable and VNC E. coli, according to their settling velocities. This work was carried out on a karstic workshop-site for which the microbial quality of water was impaired related to livestock density and septic tanks overflows. Particles characterisation was performed by estimation of their settling velocities combined with electronic microscopy observation, and solid phase cytometry (ChemScan®RDI) was carried out to quantify the viable E. coli, and thus VNC E. coli. In the karst, different populations of E. coli were coexisting related to their survival, their culturability, and their association to particles. At the sinkhole, during a rainfall event with pasture, E. coli rapidly losing their culturability after 2 days have been more frequently isolated. These isolates are mainly multiresistant to antibiotics and harbor several virulence factors. In the same time, a population of VNC E. coli (79%), associated to the "non settleable particles" (settling velocities ranging between 10-5 to 10-2 mm.s-1), mainly corresponding to colloids and organic or organo-mineral microflocs was injected in the karst system, probably corresponding to the runoff of attached-bacteria originating from cowpats. Once in the karst, the relative contribution of culturable and VNC E. coli

  20. Subvenciones para instituciones Directrices para gastos de ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    IDRC CRDI

    División de Administración de Subvenciones ... En la Parte 2 del Acuerdo de Subvención estándar de IDRC se incluyen los límites ... pagar los costos de sustitución (tiempo libre para personal académico) de los investigadores principales.

  1. Zoneamento agroclimático da palma forrageira (Opuntia sp. para o estado da Paraíba

    Directory of Open Access Journals (Sweden)

    Bergson G. Bezerra

    2014-07-01

    Full Text Available A palma forrageira tornou-se, ao longo dos anos, uma importante alternativa forrageira no semiárido brasileiro (SAB principalmente em longos períodos de estiagens. Apesar de sua magnitude para a agropecuária, seu cultivo tem dispensando tratos culturais básicos e embasamento técnico-científico quanto às suas exigências climáticas. Assim, o principal objetivo deste estudo foi elaborar o zoneamento agroclimático da cultura da palma forrageira (Opuntia sp. para o estado da Paraíba. O zoneamento agroclimático da palma forrageira foi elaborado com base nos indicadores climáticos delineados na literatura e nos dados climatológicos de precipitação e temperatura (média, máxima e mínima de 97 localidades do estado da Paraíba. De acordo com os resultados obtidos, a mesorregião da Borborema é a que apresenta as condições climáticas mais favoráveis para o cultivo da palma forrageira. As mesorregiões do Agreste, Sertão e a parte do Litoral, são aptas porém com restrições. No entanto, recomenda-se o cultivo da palma forrageira em todo o território do estado da Paraíba, exceto para a parte costeira da mesorregião do Litoral e região em torno de Areia. Em ambos os casos a inaptidão ocorre em função do excesso de precipitação.

  2. OPTIMIZACIÓN DE UN PROTOCOLO DEL AISLAMIENTO DEL ADN Y DE UN SISTEMA DE AMPLIFICACIÓN ISSR-PCR PARA Ceratozamia mexicana Brongn. (Zamiaceae.

    Directory of Open Access Journals (Sweden)

    Nadia Guadalupe Sánchez-Coello

    2012-01-01

    Full Text Available La mayoría de las cícadas contienen altas concentraciones de aceites esenciales, flavonoides, polifenoles y polisacáridos que interfieren en la extracción de ADN, causando productos de amplificación errados o inhibiendo la PCR. La optimización del aislamiento del ADN y el empleo de iniciadores de secuencias intergénicas repetidas simples (ISSRs se investigaron en Ceratozamia mexicana Brongn., una cícada mexicana en peligro de extinción. El ADN obtenido de tejido foliar fresco, con un amortiguador modificado de cetil trimetil amonio, nos permitió obtener un ADN de buena calidad, sin pigmentos coloridos o contaminantes. La modificación al protocolo de extracción de ADN, basado en CTAB, fue un prelavado por 1 h, del tejido foliar, con una solución de 0.7 M de NaCl, para facilitar la lisis celular. El ADN extraído exitosamente se amplificó por PCR, usando seis iniciadores arbitrarios ISSR. Se observaron productos de amplificación reproducibles en todas las reacciones de PCR. Nuestros resultados muestran que la implementación mejora significativamente la calidad del ADN obtenido, usando una concentración baja de iniciadores (25 pM. Se detectaron 23 bandas fuertes, nueve de las cuales fueron polimórficas. Los resultados indican que el protocolo de optimización del aislamiento del ADN y en el sistema de PCR es viable para futuros trabajos en esta especie. Este trabajo es el primer protocolo de extracción de ADN y de ISSR reportado para esta especie ornamental en peligro de extinción.

  3. Plan estratégico para la empresa Global Logistics (GL) para el periodo 2017-2019

    OpenAIRE

    Castello Manky, Luis Fernando; Grados Iturrizaga, Miguel Augusto; Hudson Okumura, Ciro

    2017-01-01

    El presente trabajo de investigación tiene como finalidad el desarrollo de un plan estratégico para la empresa Global Logistics (GL) para los años 2017 a 2019. Inicia con el análisis y diagnóstico situacional, donde se analiza el macroentorno en base al análisis PESTEL y luego se procede a analizar la industria considerando las cinco fuerzas de Porter, para, finalmente, determinar las oportunidades y amenazas que enfrenta la empresa. Una vez concluido el análisis y diagnóstico situacional, se...

  4. Procedimiento para el cierre de canteras de materiales para construcción en Cuba

    Directory of Open Access Journals (Sweden)

    Julio Montero-Matos

    2016-03-01

    Full Text Available Con el propósito de facilitar a los concesionarios de canteras de materiales para la construcción una herramienta metodológica para efectuar el cierre de estas y garantizar una minería sostenible se propuso un procedimiento en seis etapas a partir del diagnóstico realizado en 22 canteras, ubicadas en tres regiones mineras cubanas. El procedimiento busca disminuir los impactos ambientales provocados por la explotación de las canteras y ofrecer a la industria un estándar para lograr uniformidad en la planificación y ejecución del cierre con un costo mínimo.

  5. Epidemia de tuberculosis multidrogo resistente y extensivamente resistente a drogas (TB MDR/XDR en el Perú: situación y propuestas para su control

    Directory of Open Access Journals (Sweden)

    Hernán Del Castillo

    2009-07-01

    Full Text Available En este artículo se describe el estado actual de las epidemias de tuberculosis multidrogo resitente (TB MDR y tuberculosis extensivamente resistente a drogas (TB XDR en el Perú. Se realiza un análisis de nuestra situación con respecto al resto de Latinoamérica, y de la distribución temporo espacial de los casos de TB XDR en Lima. También presentamos nuestra reflexión de lo que está pasando y debe cambiar; lo que se resume en darle la importancia debida a un problema que nos está rebasando y anteponer a nuestros propios intereses, los intereses de la humanidad; por consiguiente no podemos callar, y debemos declarar a la tuberculosis como emergencia sanitaria nacional. Eso constituye una respuesta, esperamos, aún viable para el Perú.

  6. Angiographically demonstrated coronary collaterals predict residual viable myocardium in patients with chronic myocardial infarction. A regional metabolic study

    International Nuclear Information System (INIS)

    Fukai, Masumi; Ii, Masaaki; Nakakoji, Takahiro

    2000-01-01

    Angiographical demonstration of coronary collateral circulation may suggest the presence of residual viable myocardium. The development of coronary collaterals was judged according to Rentrop's classification in 37 patients with old anteroseptal myocardial infarction and 13 control patients with chest pain syndrome. The subjects with myocardial infarction were divided into 2 groups: 17 patients with the main branch of the left coronary artery clearly identified by collateral blood flow from the contralateral coronary artery [Coll (+) group, male/female 10/7, mean age 56.6 years] and 20 patients with obscure coronary trunk [Coll (-) group, male/female 16/4, mean age 54.9 years]. Thallium-201 myocardial scintigraphy and examination of local myocardial metabolism were carried out by measuring the flux of lactic acid under dipyridamole infusion load. Coronary stenosis of 99% or total occlusion was found in only 5 of 20 patients (25%) in the Coll (-) group but in 16 of 17 patients (94%) in the Coll (+) group (p<0.001). Redistribution of myocardial scintigraphy was found in 11 of 15 patients (73%) in the Coll (+) group, but only 3 of 18 patients (17%) in the Coll (-) group (p<0.01). The myocardial lactic acid extraction rate was -13.2±17.0% in the Coll (+) group, but 9.1±13.2% in the Coll (-) group (p<0.001). These results suggest that coronary collateral may contribute to minimizing the infarct area and to prediction of the presence of viable myocardium. (author)

  7. Desenvolvimento de filmes biodegradáveis para cobertura de solo, ensacamento de frutos e sacos para mudas

    OpenAIRE

    Ana Paula Bilck

    2010-01-01

    Filmes plásticos de polietileno e polipropileno derivados do petróleo são amplamente utilizados na agricultura para a confecção de estufas, sacos para produção de mudas e filmes para cobertura do solo. Estes filmes constituem um sério problema ambiental, pois devido ao seu elevado peso molecular e propriedades hidrofóbicas, possuem alta estabilidade química, levando um longo tempo para se decompor totalmente e também são de difícil reciclagem. A utilização de polímeros biodegradáveis é uma al...

  8. Comparison of viable plate count, turbidity measurement and real-time PCR for quantification of Porphyromonas gingivalis.

    Science.gov (United States)

    Clais, S; Boulet, G; Van Kerckhoven, M; Lanckacker, E; Delputte, P; Maes, L; Cos, P

    2015-01-01

    The viable plate count (VPC) is considered as the reference method for bacterial enumeration in periodontal microbiology but shows some important limitations for anaerobic bacteria. As anaerobes such as Porphyromonas gingivalis are difficult to culture, VPC becomes time-consuming and less sensitive. Hence, efficient normalization of experimental data to bacterial cell count requires alternative rapid and reliable quantification methods. This study compared the performance of VPC with that of turbidity measurement and real-time PCR (qPCR) in an experimental context using highly concentrated bacterial suspensions. Our TaqMan-based qPCR assay for P. gingivalis 16S rRNA proved to be sensitive and specific. Turbidity measurements offer a fast method to assess P. gingivalis growth, but suffer from high variability and a limited dynamic range. VPC was very time-consuming and less repeatable than qPCR. Our study concludes that qPCR provides the most rapid and precise approach for P. gingivalis quantification. Although our data were gathered in a specific research context, we believe that our conclusions on the inferior performance of VPC and turbidity measurements in comparison to qPCR can be extended to other research and clinical settings and even to other difficult-to-culture micro-organisms. Various clinical and research settings require fast and reliable quantification of bacterial suspensions. The viable plate count method (VPC) is generally seen as 'the gold standard' for bacterial enumeration. However, VPC-based quantification of anaerobes such as Porphyromonas gingivalis is time-consuming due to their stringent growth requirements and shows poor repeatability. Comparison of VPC, turbidity measurement and TaqMan-based qPCR demonstrated that qPCR possesses important advantages regarding speed, accuracy and repeatability. © 2014 The Society for Applied Microbiology.

  9. Taller para desarrollar la creatividad

    OpenAIRE

    MARTÍNEZ GUEROLA, BEATRIZ

    2015-01-01

    [ES] Partimos de la base de que la creatividad es una capacidad realmente valiosa, no solo para el ámbito artístico, si no para cualquier aspecto de la vida. Este proyecto está enfocado a un taller diseñado para que a partir de técnicas de expresión gráfico-plásticas podamos estimular y desarrollar la creatividad, principalmente en niños de tercer ciclo de primaria. Con este proyecto relacionamos el arte y la creatividad que se asocia a esté con la educación. Martínez Guerola, B. (2015). T...

  10. Sistema solar para aquecimento ambiente e aquecimento de piscinas: uma solução promissora para climas do Sul da Europa

    OpenAIRE

    Carvalho, M. J.; Neves, Ana

    2006-01-01

    A avaliação do sistema apresentado neste trabalho foi focada em sistemas que podem fornecer água quente, aquecimento ambiente e aquecimento de piscinas, e que são projectados para climas do Sul da Europa, especialmente para habitações uni-familiares. Pelas características climáticas do Sul da Europa, o aquecimento ambiente é apenas necessário para um curto período do ano, sendo para esta avaliação considerado um período de seis meses para aquecimento ambiente e um período de seis meses para a...

  11. Propuesta de un sistema de aprovechamiento de agua lluvia como alternativa para el ahorro de agua potable, en la institución educativa María Auxiliadora de Caldas, Antioquia

    Directory of Open Access Journals (Sweden)

    Palacio Castañeda Natalia

    2011-11-01

    Full Text Available El aprovechamiento del agua lluvia es una práctica interesante, tanto ambiental como económicamente, si se tiene en cuenta la gran demanda del recurso sobre las cuencas hidrográficas, el alto grado de contaminación de las fuentes superficiales y los elevados costos por el consumo de agua potable en una institución educativa. Este proyecto presenta la ingeniería conceptual de una propuesta de diseño de un si stema de aprovechamiento de agua lluvia como alternativa para el ahorro de agua potable en usos tales como la descarga de sanitarios, el lavado de zonas comunes, entre otros. Se presenta, adicionalmente, un análisis aproximado de la viabilidad técnica y económica de dicho aprovechamiento en una institución educativa del municipio de Caldas, Antioquia. Se desarrollan en este documento tres aspectos principales: el cálculo de los volúmenes disponibles de agua lluvia en la zona, la evaluación del volumen de agua potable ahorrado con el aprovechamiento del agua lluvia, la estimación del presupuesto para la construcción del sistema y la proyección del ahorro generado al utilizarlo. Los resultados sugieren que el aprovechamiento de agua lluvia es una opción técnicamente viable, pero financieramente no, pues la inversión inicial es alta, por lo que es importante buscar financiación externa para desarrollar este tipo de proyectos que representan una solución interesante para contribuir al desarrollo sostenible de la institución educativa.

  12. The amount of viable and dyssynchronous myocardium is associated with response to cardiac resynchronization therapy: initial clinical results using multiparametric ECG-gated [{sup 18}F]FDG PET

    Energy Technology Data Exchange (ETDEWEB)

    Lehner, Sebastian; Uebleis, Christopher; Haug, Alexander; Bartenstein, Peter [University of Munich, Department of Nuclear Medicine, Munich (Germany); Schuessler, Franziska; Kaeaeb, Stefan; Estner, Heidi [University of Munich, Medical Department I, Munich (Germany); Van Kriekinge, Serge D.; Germano, Guido [UCLA, Cedars-Sinai Medical Center, Los Angeles and David Geffen School of Medicine, Los Angeles, CA (United States); Hacker, Marcus [Medical University of Vienna, Division of Nuclear Medicine, Department of Biomedical Imaging and Image-guided Theraphy, Vienna (Austria)

    2013-12-15

    There is still a significant amount of patients who do not sufficiently respond to cardiac resynchronization therapy (CRT). Previous studies demonstrated that the amount of dyssynchronous myocardium was predictive of response to CRT. Otherwise, non-response is frequently associated with high amounts of scar tissue. The combination of these parameters might yield a more accurate prediction of response. We hypothesized that the probability of a CRT response increases with the presence of high amounts of ''viable and dyssynchronous'' myocardium. A total of 19 patients (17 male, 61 {+-} 10 years) underwent ECG-gated [{sup 18}F]fluorodeoxyglucose (FDG) myocardial positron emission tomography (PET) before CRT device implantation and were followed for 6 months. Response to CRT was defined as clinical improvement of at least one New York Heart Association (NYHA) class in combination with left ventricular (LV) ejection fraction (EF) improvement of >5 %. Twelve responders (71 %) and seven non-responders (29 %) were identified. For each patient bullseye maps of FDG uptake and phase analysis were calculated (QPS/QGS 2012, Cedars-Sinai, Los Angeles, CA, USA) and fused. Amounts of myocardium representing ''viable and synchronous'', ''scar and synchronous'', viable and dyssynchronous or ''scar and dyssynchronous'' myocardium were quantified by planimetric measurements of the fused bullseye maps. Responders by definition showed significant decrease in NYHA class and significant increase of LVEF. Furthermore, a significantly higher amount of viable and dyssynchronous myocardium was found as compared to non-responders (21 {+-} 13 % vs 6 {+-} 5 %; p < 0.05). Combined assessment of myocardial viability and LV dyssynchrony is feasible using multiparametric [{sup 18}F]FDG PET and could improve conventional response prediction criteria for CRT. (orig.)

  13. MODELO PARA DETERMINAR FUNÇÕES TRANSFERÊNCIA PARA ANÁLISE DE ESTABILIDADE DE VANTS

    Directory of Open Access Journals (Sweden)

    Igor Mayer Soares

    2017-01-01

    Full Text Available Neste trabalho foi apresentado um modelo para determinar as funções de transferência para análise da estabilidade longitudinal de Veículos Aéreos Não Tripulados (VANT através do desenvolvimento simplificado das equações do movimento de aeronaves. O sistema foi simplificado considerando que o motor e o arrasto na superfície de comando não influenciam na dinâmica do veículo, pois o mesmo é significativamente menor que um avião. Analisamos também a resposta em função do tempo para as deflexões das superfícies de comando. Nos resultados apresentados é possível perceber que mesmo simples, o modelo oferece uma boa aproximação para análise dinâmica de VANTs e predizer sua estabilidade e qualidade de voo.

  14. Metodología lúdica para la enseñanza de la programación dinámica determinista en un contexto universitario

    Directory of Open Access Journals (Sweden)

    Carlos Mauricio Zuluaga-Ramírez

    2016-01-01

    Full Text Available En este artículo se presentan los resultados de un proceso investigativo desarrollado por el Grupo en la Enseñanza de la Investigación de Operaciones (GEIO, perteneciente a la Facultad de Ingeniería Industrial de la Universidad Tecnológica de Pereira – Colombia, en donde la lúdica es planteada como metodología de enseñanza para generar micromundos que permitieran a estudiantes del ámbito universitario interiorizar conceptos matemáticos difíciles de comprender por métodos reduccionistas. La investigación documentada se desarrolló con un grupo de alumnos de la Facultad de Ingeniería Industrial, con los cuales se trabajó el concepto de programación dinámica determinista, enfocada al balanceo de línea empleando una lúdica de un proceso productivo textil. La aplicación práctica fue evaluada mediante el proceso de validación de expertos y permitió concluir que la metodología lúdica propuesta por GEIO es clara, pertinente, viable, coherente y constituye una herramienta para representar sistemas reales, donde los estudiantes pueden asociar fácilmente conceptos teóricos a situaciones prácticas, llegando a generar aprendizaje significativo gracias a la interacción del participante con el contexto simulado.

  15. Anaerobic digestion of vinasses: determination of biomass; Digestion anaerobia de vinazas de vino: determinacion de la biomasa viable

    Energy Technology Data Exchange (ETDEWEB)

    Solera, R.; Romero, L.; Salles, D.

    2002-07-01

    In this paper, we studied the thermophilic anaerobic treatment of vinasses. The viable bacterial population was quantified by soil medium plating techniques, employing an anaerobic chamber for spreading into plates and subsequent incubation of inoculated plates. This technique has been applied to the measurement of the microbial population contained in both single and tow-stage, laboratory-scale reactors. In the single-stage process the main reaction steps-acidogenesis and methano genesis-take place in the same reactor, while in the two-stage process they take place in separate reactors. (Author) 11 refs.

  16. MODELO PARA LA CREACIÓN DEL CONOCIMIENTO PARA PYMES

    Directory of Open Access Journals (Sweden)

    Marino Valencia Rodríguez

    2009-01-01

    Full Text Available La creciente importancia del conocimiento, como nuevo factor de producción, hace que la creación y transferencia se convierta en una de las principales prioridades de las organizaciones. El presente artículo describe tanto los fundamentos de la teoría de los recursos y capacidades de la empresa como el enfoque de la gestión del conocimiento, con sus procesos de generación y transferencia del conocimiento. Estos sirven de guía básica teórica para alcanzar el siguiente objetivo: Diseñar un modelo de generación y transferencia de conocimiento para los procesos de dirección, gestión humana y del conocimiento para PyMES, con el fin de que éstas alcancen mayores niveles de competitividad. El modelo se diseña, a partir del direccionamiento estratégico de la organización. Está conformado por tres etapas, articuladas con elementos y actividades. Las acciones se enfocan en dos factores clave de éxito: la cultura organizacional y la formación. La utilización del modelo permitirá a las PyMES optimizar los recursos y capacidades disponibles, lo cual se refleja en el desempeño del trabajador y en el fortalecimiento de la cultura organizacional.

  17. Flora da Paraíba, Brasil: Loganiaceae Flora of Paraíba, Brazil: Loganiaceae

    Directory of Open Access Journals (Sweden)

    Kiriaki Nurit

    2005-06-01

    Full Text Available Neste trabalho apresenta-se o tratamento taxonômico da família Loganiaceae, como parte do projeto "Flora da Paraíba", que vem sendo realizado com o objetivo de identificar e catalogar as espécies da flora local. Realizouse coletas e observações de campo para as identificações, descrições e ilustrações botânicas que foram efetuadas após estudos morfológicos, com o auxílio da bibliografia especializada, complementados pela análise de fotos de tipos, espécimes dos herbários EAN, JPB e IPA, e comparação com material identificado por especialistas. Registrou-se para a Paraíba quatro espécies: Spigelia anthelmia L., com ampla distribuição, e três espécies de Strychnos, somente coletadas em remanescentes de Mata Atlântica, S. atlantica Krukoff & Barneby, S. parvifolia A. DC. e S. trinervis (Vell. Mart.This work constitutes a taxonomic treatment of the Loganiaceae family as part of the project "Flora da Paraíba", which have been carried out with the objective to identify and catalogue the species of the local flora. The botanical identifications and illustrations were made by morphological studies supported by bibliography, analysis of the types, specimens from herbaria EAN, JPB and IPA, and comparison with samples previously identified by specialists, complemented by field observations. Four species of Loganiaceae belonging two genera were found in State of Paraíba: Spigelia anthelmia L. that has wide distribution and three species of Strychnos, found on remains of Atlantic forest, which are S. atlantica Krukoff & Barneby, S. parvifolia A. DC. and S. trinervis (Vell. Mart.

  18. Educación para emprender una alternativa para el siglo XXI

    Directory of Open Access Journals (Sweden)

    Luis Gerardo Rodríguez Gómez

    2015-05-01

    Full Text Available RESUMEN El artículo describe la experiencia educativa del programa Físico- Deportivo orientado a estimular la actitud para emprender dentro de una concepción de forma integral, mediante Asignaturas de contenido Sico- físico, como parte del desarrollo curricular en Facultades de Administración de Empresas e ingeniería de Sistemas en la Escuela de Administración de Negocios (EAN. El desarrollo temático hace énfasis en un proceso orientado  a superar formas de educación encaminadas al logro de habilidades técnicas para el ejercicio de un empleo y propone desde  la educación delas conductas  motrices, modos de encausar la energía sicosomática con el fin de asimilar la incertidumbre del momento histórico que se vive en los países de lento desarrollo, para corresponder a un mundo que empuña la bandera de la diversidad, el cambio acelerado y la competencia tecnológica.

  19. A Rapid Method for Quantifying Viable Mycobacterium avium subsp. paratuberculosis in Cellular Infection Assays

    Science.gov (United States)

    Pooley, Hannah B.; de Silva, Kumudika; Purdie, Auriol C.; Begg, Douglas J.; Whittington, Richard J.

    2016-01-01

    ABSTRACT Determining the viability of bacteria is a key outcome of in vitro cellular infection assays. Currently, this is done by culture, which is problematic for fastidious slow-growing bacteria such as Mycobacterium avium subsp. paratuberculosis, where it can take up to 4 months to confirm growth. This study aimed to identify an assay that can rapidly quantify the number of viable M. avium subsp. paratuberculosis cells in a cellular sample. Three commercially available bacterial viability assays along with a modified liquid culture method coupled with high-throughput quantitative PCR growth detection were assessed. Criteria for assessment included the ability of each assay to differentiate live and dead M. avium subsp. paratuberculosis organisms and their accuracy at low bacterial concentrations. Using the culture-based method, M. avium subsp. paratuberculosis growth was reliably detected and quantified within 2 weeks. There was a strong linear association between the 2-week growth rate and the initial inoculum concentration. The number of viable M. avium subsp. paratuberculosis cells in an unknown sample was quantified based on the growth rate, by using growth standards. In contrast, none of the commercially available viability assays were suitable for use with samples from in vitro cellular infection assays. IMPORTANCE Rapid quantification of the viability of Mycobacterium avium subsp. paratuberculosis in samples from in vitro cellular infection assays is important, as it allows these assays to be carried out on a large scale. In vitro cellular infection assays can function as a preliminary screening tool, for vaccine development or antimicrobial screening, and also to extend findings derived from experimental animal trials. Currently, by using culture, it takes up to 4 months to obtain quantifiable results regarding M. avium subsp. paratuberculosis viability after an in vitro infection assay; however, with the quantitative PCR and liquid culture method

  20. Examination of an indicative tool for rapidly estimating viable organism abundance in ballast water

    Science.gov (United States)

    Vanden Byllaardt, Julie; Adams, Jennifer K.; Casas-Monroy, Oscar; Bailey, Sarah A.

    2018-03-01

    Regulatory discharge standards stipulating a maximum allowable number of viable organisms in ballast water have led to a need for rapid, easy and accurate compliance assessment tools and protocols. Some potential tools presume that organisms present in ballast water samples display the same characteristics of life as the native community (e.g. rates of fluorescence). This presumption may not prove true, particularly when ships' ballast tanks present a harsh environment and long transit times, negatively impacting organism health. Here, we test the accuracy of a handheld pulse amplitude modulated (PAM) fluorometer, the Hach BW680, for detecting photosynthetic protists at concentrations above or below the discharge standard (< 10 cells·ml- 1) in comparison to microscopic counts using fluorescein diacetate as a viability probe. Testing was conducted on serial dilutions of freshwater harbour samples in the lab and in situ untreated ballast water samples originating from marine, freshwater and brackish sources utilizing three preprocessing techniques to target organisms in the size range of ≥ 10 and < 50 μm. The BW680 numeric estimates were in agreement with microscopic counts when analyzing freshly collected harbour water at all but the lowest concentrations (< 38 cells·ml- 1). Chi-square tests determined that error is not independent of preprocessing methods: using the filtrate method or unfiltered water, in addition to refining the conversion factor of raw fluorescence to cell size, can decrease the grey area where exceedance of the discharge standard cannot be measured with certainty (at least for the studied populations). When examining in situ ballast water, the BW680 detected significantly fewer viable organisms than microscopy, possibly due to factors such as organism size or ballast water age. Assuming both the BW680 and microscopy with FDA stain were measuring fluorescence and enzymatic activity/membrane integrity correctly, the observed discrepancy

  1. Flexible Nanosomes (SECosomes) Enable Efficient siRNA Delivery in Cultured Primary Skin Cells and in the Viable Epidermis of Ex Vivo Human Skin

    NARCIS (Netherlands)

    Geusens, Barbara; Van Gele, Mireille; Braat, Sien; De Smedt, Stefaan C.; Stuart, Marc C. A.; Prow, Tarl W.; Sanchez, Washington; Roberts, Michael S.; Sanders, Niek N.; Lambert, Jo

    2010-01-01

    The extent to which nanoscale-engineered systems cross intact human skin and can exert pharmacological effects in viable epidermis is controversial. This research seeks to develop a new lipid-based nanosome that enables the effective delivery of siRNA into human skin. The major finding is that an

  2. La carrera profesional para el profesorado

    Directory of Open Access Journals (Sweden)

    José Gimeno Sacristán

    2010-01-01

    Full Text Available La carrera del profesorado es un instrumento para el reconocimiento del mérito de los profesores en el desarrollo de su profesionalidad -lo cual supone atender a la mejora de sus capacidades cognitivas como a su competencia reflexivo-práctica- cuyos fines son la motivación para el ejercicio de sus funciones, reforzar su compromiso con la educación en general y como forma de reconocer con justicia el mérito de los mejores profesionales. Desde un punto de vista estrictamente educativo, la carrera debe servir para la mejora de las prácticas de enseñanza que incidan en la mejora de los aprendizajes. Esta pretensión nos exige explicitar los criterios para determinar lo que entendemos como buenas prácticas y precisar los criterios para su evaluación. La complejidad de la enseñanza requiere metodologías adecuadas para abordar procesos de índole cualitativa. La regulación de la carrera docente tiene que superar tanto el cuantitativismo fácil en los métodos y la regulación burocrática de niveles de calidad, así como contemplar un conjunto de incentivos no solamente salariales.

  3. 10 conference and Latin American turbomachinery exhibition; 10 congreso y exposicion latinoamericana de turbomaquinaria

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-11-15

    This conference was done to promote the exchange of experiences among specialists, users, manufactures and research institutions in the field of the turbomachinery. To spread the last researches and developments focus to the design and maintenance of steam, gas and hydraulic turbines, axial compressors and centrifugal pumps, fans, electric motors, auxiliary equipment and cogeneration. [Spanish] Este congreso se realizo con el fin de promover el intercambio de experiencias entre especialistas, usuarios, fabricantes e institutos de investigacion en el campo de la turbomaquinaria. Divulgar las ultimas investigaciones y desarrollos enfocados al diseno y mantenimiento de turbinas de vapor, de gas e hidraulicas, compresores axiales y centrifugos, bombas, ventiladores, motores electricos, equipos auxiliares y cogeneracion.

  4. Viable Species of Flamella (Amoebozoa: Variosea) Isolated from Ancient Arctic Permafrost Sediments.

    Science.gov (United States)

    Shmakova, Lyubov; Bondarenko, Natalya; Smirnov, Alexey

    2016-02-01

    Six viable strains of amoebae belonging to the genus Flamella (Amoebozoa, Variosea) were isolated from permafrost sediments sampled in the Russian Arctic region. Two of them are from late Pleistocene permafrost in North-East Siberia, and four--from Holocene and late Pleistocene in North-West Siberia. Light- and electron-microscopic study and molecular phylogeny show that these isolates represent two new species belonging to the genus Flamella. Both species are cyst-forming. This is a remarkable case of high resistance of protozoan cysts, allowing them to survive and recover an amoebae population after a very long, geologically significant period of rest; a "snapshot" of evolution in time. This study directly shows for the first time that amoeba cysts can be conserved not only for years and decades but for many thousand years and then recover, contributing to the formation of an active microbial community. We propose to name the new species as Flamella pleistocenica n.sp. and Flamella beringiania n.sp. Phylogenetic analysis shows that the genus Flamella is a robust and potentially species-rich group of Variosea. Copyright © 2015 Elsevier GmbH. All rights reserved.

  5. Making decentralised systems viable: a guide to managing decentralised assets and risks.

    Science.gov (United States)

    Willetts, J; Fane, S; Mitchell, C

    2007-01-01

    Decentralised systems have the potential to provide a viable option for long term sustainable management of household wastewater. Yet, at present, such systems hold an uncertain status and are frequently omitted from consideration. Their potential can only be realised with improved approaches to their management, and improved methods to decision-making in planning of wastewater systems. The aim of this paper is to demonstrate the value of a novel framework to guide the planning of decentralised systems so that asset management and risk management are explicitly considered. The framework was developed through a detailed synthesis of literature and practice in the area of asset management of centralised water and wastewater systems, and risk management in the context of decentralised systems. Key aspects of the framework are attention to socio-economic risks as well as engineering, public health and ecological risks, the central place of communication with multiple stakeholders and establishing a shared asset information system. A case study is used to demonstrate how the framework can guide a different approach and lead to different, more sustainable outcomes, by explicitly considering the needs and perspectives of homeowners, water authorities, relevant government agencies and society as a whole.

  6. 137. Intervención de cabrol. ¿Cómo? ¿Cuándo?

    Directory of Open Access Journals (Sweden)

    A. Lahoz Tornos

    2012-04-01

    Conclusión: En casos complejos de reintervenciones en pacientes con anuloectasia aórtica que presenten dificultad para movilizar las pastillas coronarias para la realización de un Bentall, la opción quirúrgica que nos parece más viable es la intervención de Cabrol.

  7. Tiempo para un cambio

    Science.gov (United States)

    Woltjer, L.

    1987-06-01

    En la reunion celebrada en diciembre dei ano pasado informe al Consejo de mi deseo de terminar mi contrato como Director General de la ESO una vez que fuera aprobado el proyecto dei VLT, que se espera sucedera hacia fines de este aAo. Cuando fue renovada mi designacion hace tres aAos, el Consejo conocia mi intencion de no completar los cinco aAos dei contrato debido a mi deseo de disponer de mas tiempo para otras actividades. Ahora, una vez terminada la fase preparatoria para el VLT, Y habiendose presentado el proyecto formalmente al Consejo el dia 31 de marzo, y esperando su muy probable aprobacion antes dei termino de este ano, me parece que el 10 de enero de 1988 presenta una excelente fecha para que se produzca un cambio en la administracion de la ESO.

  8. Leasing: una alternativa de financiamiento para PYMES

    OpenAIRE

    Bedjan, María Belén

    2008-01-01

    En la actualidad las pequeñas y medianas empresas encuentran dificultades para acceder a las líneas tradicionales de créditos bancarios. Por otro lado las empresas necesitan actualizarse constantemente para poder ser competitivas en el mercado. Es aquí donde el leasing nace como una alternativa de financiación para las PyMEs. Este trabajo pretendió demostrar la conveniencia financiera del leasing con respecto a los préstamos bancarios para la compra de maquinarias agrícolas ...

  9. Estrategia para elevar la motivación laboral; factor imprescindible para mejorar nuestra productividad.

    Directory of Open Access Journals (Sweden)

    Reina de los Ángeles Carballé Piñón

    2015-12-01

    Full Text Available Este trabajo "Estrategia para elevar la motivación laboral; factor imprescindible para mejorar nuestra productividad" resulta de la investigación realizada para tesis de maestría; se realizó el estudio de 2 entidades, nos permitió conocer las principales posiciones que sobre motivación existen, factores que la influencian, cómo incide en el desempeño laboral y que acciones diseñar para elevarla. Las 2 entidades seleccionadas, claves en el desempeño empresarial son un centro de interfase y un centro generador de conocimientos, nuestro objetivo principal es sugerir propuestas que eleven la motivación de los trabajadores del CIGET(centro de interfase y la Universidad de Sancti Spíritus(centro generador de conocimientos por desempeñar una mejor labor y lograr así aumento de la eficacia, eficiencia y productividad; este se concreta específicamente en determinar qué factores ocasionan desmotivación en los trabajadores del CIGET y la Universidad, identificar aquellos factores que realmente motivan a estas personas, de manera individual como colectivamente y trazar una estrategia con el objetivo de elevar la motivación de los trabajadores hacia la actividad que realizan y dotar a estos de herramientas para la identificación de actitudes positivas o negativas en el ámbito laboral y cómo potenciarlas o minimizarlas según sea el caso.

  10. Competencia para desarrollar la alfabetización digital

    OpenAIRE

    González Rodríguez, José Santiago; Navarrete Aguirre, Mariana; Molina Zacate, Ma. Gabriela

    2011-01-01

    Introducción, Objetivos Generales, Temario: (1) ¿Qué significa la competencia para desarrollar la alfabetización digital? (2) ¿Por qué es relevante que adquiera la competencia para desarrollar la alfabetización digital en mi formación? (3) ¿Cómo aprendo la competencia para desarrollar la alfabetización digital? (4) ¿Cómo aplico la competencia para desarrollar la alfabetización digital? (5) ¿Cómo puedo autoevaluar mi competencia para desarrollar la alfabetización digital (...

  11. Development of a propidium monoazide-polymerase chain reaction assay for detection of viable Lactobacillus brevis in beer.

    Science.gov (United States)

    Ma, Yanlin; Deng, Yang; Xu, Zhenbo; Liu, Junyan; Dong, Jianjun; Yin, Hua; Yu, Junhong; Chang, Zongming; Wang, Dongfeng

    The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. However, one of the drawbacks is the inability to differentiate between live and dead cells. In this paper, the combination of propidium monoazide (PMA) pretreatment and conventional PCR had been described. The established PMA-PCR identified beer spoilage Lactobacillus brevis based not on their identity, but on the presence of horA gene which we show to be highly correlated with the ability of beer spoilage LAB to grow in beer. The results suggested that the use of 30μg/mL or less of PMA did not inhibit the PCR amplification of DNA derived from viable L. brevis cells. The minimum amount of PMA to completely inhibit the PCR amplification of DNA derived from dead L. brevis cells was 2.0μg/mL. The detection limit of PMA-PCR assay described here was found to be 10 colony forming units (CFU)/reaction for the horA gene. Moreover, the horA-specific PMA-PCR assays were subjected to 18 reference isolates, representing 100% specificity with no false positive amplification observed. Overall the use of horA-specific PMA-PCR allows for a substantial reduction in the time required for detection of potential beer spoilage L. brevis and efficiently differentiates between viable and nonviable cells. Copyright © 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  12. Motivacao para o tratamento. Dados psicometricos da escala SOCRATES 8D e estrategias psicoterapeuticas para motivar para o tratamento

    OpenAIRE

    Janeiro, Luís; Faísca, Luís

    2008-01-01

    A motivação para o tratamento é considerada um factor fundamental para o processo de mudança e tratamento do toxicodependente. Prochaska, Diclemente, e Norcross (1992) consideram que a motivação que o sujeito possui no início do tratamento determina directamente os resultados alcançados no mesmo. Diclemente, Bellino, e Neavins (1999) acrescentam, ainda, que a estreita relação entre os resultados obtidos no tratamento e a motivação aquando do seu início pode ser medida pela formação precoce de...

  13. La estrategia educativa 2020 o las limitaciones del Banco Mundial para promover el "aprendizaje para todos"

    Directory of Open Access Journals (Sweden)

    Antoni Verger

    2011-12-01

    Full Text Available LA NUEVA Estrategia Educativa 2020 del Banco Mundial establece las prioridades de reforma educativa en países en vías de desarrollo para la década siguiente. El título explícito de la estrategia, Aprendizaje para Todos, es un claro reconocimiento de que, más allá de políticas centradas en el acceso, se debe hacer algo más para asegurar que la educación derive en experiencias positivas de aprendizaje. Sin embargo, como este artículo sostiene, las opciones de políticas explícitas y latentes en la Estrategia 2020 no son las más adecuadas para lograr el Aprendizaje para Todos. El artículo desarrolla tres tipos de argumentos al respecto. El primero se refiere al fuerte apego del Banco a un conocimiento disciplinario y un enfoque metodológico que es insuficiente para entender lo que aprenden los niños en la escuela y por qué. El segundo argumento se refiere al sesgo pro-mercado de la Estrategia por lo que respecta a la reforma del sector público y a nuevas formas de oferta educativa. En tercer lugar, el artículo señala las principales ausencias de la Estrategia, con especial atención a las omisiones relacionadas con la compleja relación entre educación y pobreza.

  14. Inteligencia Emocional para tiempos de crisis

    OpenAIRE

    Fernández-Berrocal, Pablo

    2014-01-01

    La Inteligencia Emocional se concibe como un conjunto de habilidades, tanto básicas como complejas, dirigidas a “unificar las emociones y el razonamiento”, esto es, usar las emociones para facilitar el razonamiento y los procesos de pensamiento, y usar nuestro razonamiento para pensar de forma inteligente acerca de nuestras emociones (Mayer y Salovey, 1997). En concreto, la IE se define como una habilidad mental que incluye “la capacidad para percibir, valorar y expresar las emociones con...

  15. GRASP para o PQA: um limite de aceitação para soluções iniciais

    Directory of Open Access Journals (Sweden)

    Range Maria Cristina

    2000-01-01

    Full Text Available O Problema Quadrático de Alocação (PQA pertence à classe dos problemas NP-Hard e desafia os pesquisadores tanto em sua teoria quanto em sua parte computacional. Pela sua alta complexidade muitos métodos heurísticos têm sido desenvolvidos para tentar resolvê-lo aproximadamente. A metaheurística GRASP (greedy randomized adaptive search procedures se mostrou bastante eficiente. Neste trabalho, uma proposta para descartar soluções iniciais supostamente ruins é apresentada com base na normalização de custos calculadas num intervalo entre limites de solução. Para este GRASP restrito, foi observada uma redução do tempo computacional para encontrar as soluções ótimas ou soluções viáveis de boa qualidade quando comparado ao GRASP original.

  16. Paisajes para un desarrollo sustentable y participativo

    Directory of Open Access Journals (Sweden)

    Rafael Mata Olmo

    2014-11-01

    Para terminar, el texto recoge una experiencia de proyecto territorial de paisaje, la del Plan Insular de Menorca (Islas Baleares, España, aprobado en 2003. Se sintetizan sus determinaciones sobre la protección de determinados terrenos por sus altos valores paisajísticos, sus directrices para que las políticas sectoriales (turismo, agricultura, infraestructuras, etc. incorporen criterios paisajísticos, y sus iniciativas para la gestión y mejora del paisaje y para el fomento del acceso público a su contemplación y disfrute.

  17. Sistema de realidad aumentada para aplicaciones Android

    OpenAIRE

    Fernández Sánchez, Natalia Mercedes

    2012-01-01

    El objetivo principal de este proyecto es la creación de un sistema de realidad aumentada para el sistema operativo Android con idea de que sea utilizado para la creación de un videojuego, pero que a su vez sea lo suficientemente versátil como para poder utilizarse en otro tipo de aplicaciones. De este objetivo principal podemos obtener los objetivos secundarios: - Conocer las características que ofrecen los teléfonos móviles y como éstas pueden ser aprovechadas para la crea...

  18. Comparative study of 201Tl reinjection tomography and combined use of 13N-ammonia and 18F-fluorodeoxyglucose positron emission tomography for detection of viable myocardium

    International Nuclear Information System (INIS)

    Wang Lijuan; Qi Guoxian; Hu Jian

    2001-01-01

    Objective: To compare the clinical value of 201 Tl reinjection tomography and combined use of 13 N-ammonia (NH 3 ) regional myocardial perfusion and 18 F-fluorodeoxyglucose (FDG) positron emission tomography transfer function and for the myocardium. Methods: The authors performed exercise 201 Tl SPECT (Tl SPECT) on 20 patients with myocardial infarction and obtained standard 201 Tl exercise-redistribution images. 201 Tl was reinjected immediately after redistribution study, and the images were then acquired as also. The 20 patients also underwent PET imaging with 13 N-NH 3 and 18 F-FDG (NH 3 -FDG PET) within one week of the above study. Vertical and horizontal long axis cuts were divided into nine segments, and uptakes of radioisotope were evaluated into four classes visually (Defect score, DS) (0:normal uptake, 3:severely reduced). Quantitative evaluation was performed on the vertical and horizontal long axis cuts of 201 Tl redistribution, reinjection and FDG PET images using regions of interest. The relative myocardial 201 Tl uptake and 18 F-FDG uptake (% ID) were expressed as the percentage of this reference region accounted for of the maximal counts. Results: In 48 segments identified as viable by the NH 3 -FDG PET images, 45 (93.8%) segments were also identified as viable by the 201 Tl redistribution imaging. Of 24 segments with severe irreversible defects on the 201 Tl redistribution imaging, 87.5% of the segments were concordantly confirmed by 201 Tl reinjection and NH 3 -FDG PET images with 37.5% identified as viable and 50% identified as scar. There was no significant difference in the average % ID, furthermore, regression analysis revealed a high positive correlation in the segmental % ID between 201 Tl reinjection and FDG PET images in the 61 segments with myocardial infarction (r = 0.722). Conclusions: The results show that 201 Tl reinjection tomography is of similar accuracy with NH 3 -FDG PET for detection of viable myocardium and possess important

  19. Marco de referencia para el desarrollo de un sistema de apoyo para la toma de decisiones para la gestión de inventarios

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Castro Zuluaga

    2014-01-01

    Full Text Available El principal objetivo de este artículo es presentar un marco de referencia para desarrollar un sistema de soporte a la toma de decisiones para la gestión de inventarios que permita definir técnicamente los parámetros de una política de control de inventario específica. El modelo propuesto puede ser utilizado de forma genérica en casi cualquier tipo de industria, el cual incluye las entradas fundamentales, una descripción de los procesos internos requeridos y las salidas esperadas. En la investigación se utilizaron métodos inductivos-deductivos para definir el marco de referencia propuesto, el cual se fundamenta en dos indicadores clave en la gestión de inventario: niveles de servicio y costos. Finalmente, mediante una simulación es posible concluir que utilizando los parámetros teóricos para el modelo de inventario punto de reorden-cantidad fija, que son encontrados por el marco de referencia propuesto, se obtienen aproximadamente los valores esperados tanto de los niveles de servicio como de los costos.

  20. Meios e fins do desenvolvimento para Celso Furtado

    Directory of Open Access Journals (Sweden)

    Fabio Antonio de Campos

    2014-10-01

    Full Text Available O objetivo desse artigo é expor o conceito de desenvolvimento para Celso Furtado. Para tanto, nos interessa apresentar o desenvolvimento nacional como um fim em sua abordagem sobre a formação social brasileira Para atingir tal estágio, os meios para o autor seriam assegurados por um típico desenvolvimento capitalista. Dentro dessa problemática, mostraremos também o alcance e os limites dessa concepção para o caráter dependente e subdesenvolvido do capitalismo brasileiro.

  1. Metodología para un proceso de planeación estratégica para las PYMES

    OpenAIRE

    Bonilla Zhindón, Daniela

    2014-01-01

    Todas las organizaciones, grandes, medianas, pequeñas, públicas y privadas, requieren de un plan estratégico estructurado para determinar las posiciones futuras en las que desean estar, por este motivo el propósito del presente estudio es construir una metodología transversal para el levantamiento de la planeación estratégica para el sector de calzado en la ciudad de Cuenca, mediante un análisis del estado en la que se encuentran las empresas en cuanto al proceso de planificación estratégica,...

  2. Coccocypselum pulchellum (Rubiaceae, nuevo registro para Argentina

    Directory of Open Access Journals (Sweden)

    Elsa L. Cabral

    2007-01-01

    Full Text Available Se cita Coccocypselum pulchellum por primera vez para Argentina, en Predio Guaraní, Misiones. Esta es la tercera especie de Coccocypselum registrada para la flora de Argentina junto con C. hasslerianum y C. lanceolatum. Se incluyen descripciones, ilustraciones y una clave para reconocer las tres especies argentinas de Coccocypselum.

  3. Modelo para determinar as exigências de proteína para poedeiras Modelling protein utilization in laying hens

    Directory of Open Access Journals (Sweden)

    Nilva Kazue Sakomura

    2002-11-01

    Full Text Available O objetivo do trabalho foi elaborar um modelo para estimar as exigências de proteína bruta (PB para poedeiras leves, usando o método fatorial. Para determinar as exigências de proteína bruta (PB para manutenção foi utilizada a técnica do balanço de nitrogênio. A exigência de proteína bruta para o ganho de peso foi determinada em função do conteúdo de nitrogênio na carcaça e a eficiência de utilização do nitrogênio da dieta. A exigência de PB, para produção de ovos, foi determinada considerando o teor de PB determinado nos ovos e a eficiência de deposição do nitrogênio no ovo. A partir dos valores das exigências para manutenção, para o ganho e produção foi elaborada uma equação para predizer as exigências diárias de PB (g/ ave/ dia para poedeiras: PB = 1,94. P0,75 + 0,48.G + 0,301.O, em que P = peso corporal (kg, G = ganho de peso diário (g/dia e O = massa de ovos produzida (g/ave/dia.The objective of this study was to determine a model for crude protein requirements (CP for laying hen by the factorial method. The protein maintenance requirement was determine by the nitrogen balance technique . The crude protein requirement for weight gain was determined based on body nitrogen content and nitrogen efficiency for body deposition. The crude protein requirement for egg production was determined based on the nitrogen content of eggs and nitrogen efficiency for egg deposition. Considering the requirements for maintenance, egg production and weight gain, it was elaborated a protein requirement model for laying hen: PB = 1.94xW.75 + 0.480xG + 0,301x E, where PB = requirement (g/bird/day, W = body weight (kg, G = daily weight gain (g/day and E = egg mass (g/bird/day.

  4. Passive superconductor: A viable method of controlling magnetization multipoles in the SSC dipole

    International Nuclear Information System (INIS)

    Green, M.A.

    1989-02-01

    At injection, the magnetization of the superconductor produces the dominant field error in the SSC dipole magnets. The field generated by magnetization currents in the superconductor is rich in higher symmetric multipoles (normal sextupole, normal decapole, and so on). Pieces of passive superconductor properly located within the bore of the dipole magnet can cancel the higher multipoles generated by the SSC dipole coils. The multipoles generated by the passive superconductor (predominantly sextupole and decapole) are controlled by the angular and radial location of the superconductor, the volume of superconductor, and the size of the superconducting filaments within the passive conductor. This paper will present the tolerances on each of these factors. The paper will show that multipole correction using passive superconductor is in general immune to the effects of temperature and magnetization decay due to flux creep, provided that dipole superconductor and the passive correction superconductor are properly specified. When combined with a lumped correction system, the passive superconductor can be a viable alternative to continuous correction coils within the SSC dipoles. 20 refs., 8 figs., 2 tabs

  5. Passive superconductor a viable method of controlling magnetization multipoles in the SSC dipole

    International Nuclear Information System (INIS)

    Green, M.A.

    1989-01-01

    At injection, the magnetization of the superconductor produces the dominant field error in the SSC dipole magnets. The field generated by magnetization currents in the superconductor is rich in higher symmetric multipoles (normal sextupole, normal decapole, and so on). Pieces of passive superconductor properly located within the bore of the dipole magnet can cancel the higher multipoles generated by the SSC dipole coils. The multipoles generated by the passive superconductor (predominantly sextupole and decapole) are controlled by the angular and radial location of the superconductor, the volume of superconductor, and the size of the superconducting filaments within the passive conductor. This paper will present the tolerances on each of these factors. The paper will show that multipole correction using passive superconductor is in general immune to the effects of temperature and magnetization decay due to flux creep, provided that dipole superconductor and the passive correction superconductor are properly specified. When combined with a lumped correction system, the passive superconductor can be a viable alternative to continuous correction coils within the SSC dipoles. 20 refs., 8 figs., 2 tabs

  6. Tuberculose em Salvador: custos para o sistema de saúde e para as famílias

    Directory of Open Access Journals (Sweden)

    Costa João G

    2005-01-01

    Full Text Available OBJETIVO: A tuberculose é uma das maiores causas de mortalidade no mundo, porém seus efeitos econômicos são pouco conhecidos. O objetivo do estudo foi o de estimar os custos do tratamento e prevenção da tuberculose para o sistema de saúde (público e privado e para as famílias. MÉTODOS: O estudo foi realizado no município de Salvador, BA, em 1999. Os dados para estimação dos custos para o sistema de saúde foram coletados nas secretarias de saúde, centros de saúde e em uma entidade filantrópica. Os custos públicos e privados foram estimados pela metodologia da contabilidade de custos. Os dados de custos para as famílias foram coletados por meio de questionários e incluem despesas com transporte, alimentação e outros, bem como as perdas de renda associadas à doença. RESULTADOS: O custo médio para tratamento de um caso novo de tuberculose foi de aproximadamente R$186,00 (US$103; para o tratamento de um paciente multiresistente o custo foi 27 vezes mais alto. Os custos para o serviço público corresponderam a 65% em internações, 32% em tratamento e apenas 3% em prevenção. As famílias comprometeram cerca de 33% da sua renda com despesas relacionadas a tuberculose. CONCLUSÕES: Apesar do fato das famílias não terem que pagar por medicamentos e tratamento, dado que este serviço é oferecido pelo Estado, os custos familiares ligados a perda de rendimentos devido a doença foram muito elevados. A proporção utilizada em prevenção pelo serviço público é pequena. Um maior investimento em campanhas de prevenção poderia não somente diminuir o número de casos, mas também, levar a um diagnósticos precoce, diminuindo os custos associados à hospitalização. A falta de um sistema integrado de custos não permite a visualização dos custos nos diversos setores.

  7. UNA PROPUESTA EVALUATIVA PARA ACTIVIDADES DE EDUCACIÓN AMBIENTAL PARA LA SOSTENIBILIDAD

    Directory of Open Access Journals (Sweden)

    Rosa M. Medir Huerta

    2016-01-01

    Full Text Available El propósito de este artículo es presentar una propuesta evaluativa para ac - tividades de educación ambiental para la sostenibilidad en el ámbito escolar ofrecidas desde entidades externas a la escuela. Para ello, se realiza una eva - luación real de un programa educativo de una administración pública que se orienta al refuerzo del currículo de educación infantil, primaria y secundaria. El objetivo de la evaluación es diagnosticar la presencia, en las actividades estudiadas, de principios clave de la educación ambiental para la sosteni - bilidad identificados por el equipo de investigación a partir de referencias internacionales. La metodología es la investigación evaluativa de programas dentro del paradigma crítico de la investigación educativa y con herramientas de tipo cuantitativo. Se utiliza, básicamente, un cuestionario expresamente diseñado que valora la presencia y la ausencia de los principios establecidos de la educación ambiental para la sostenibilidad. Los resultados nos aportan nuevas informaciones acerca de las fortalezas y las debilidades del desarrollo de actividades de educación ambiental. Se llega a una valoración cuantificada de los principios definidos en tres grupos, según su necesidad de mejora, y se determina que los principios mejor tratados en las actividades son los que se relacionan con los contenidos disciplinares más clásicos, tratados con buenos y distintos métodos didácticos. En cambio, las debilidades detectadas se refieren a aspectos cruciales de la educación ambiental tales como experimentar nuevas sensaciones, aprender a buscar información, pensamiento crítico, sentimiento de pertenencia a un lugar, trabajo en colaboración, adquirir una visión global de la realidad, y reflexionar sobre la vida cotidiana y sobre el futuro deseado.

  8. Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization

    Czech Academy of Sciences Publication Activity Database

    Krajčovič, T.; Bučko, M.; Vikartovská, A.; Lacík, I.; Uhelská, L.; Chorvát, D.; Neděla, Vilém; Tihlaříková, Eva; Gericke, M.; Heinze, T.; Gemeiner, P.

    2017-01-01

    Roč. 7, č. 11 (2017), s. 353-364 ISSN 2073-4344 Institutional support: RVO:68081731 Keywords : polyelectrolyte complex beads * environmental scanning electron microscopy * confocal laser scanning microscopy * Baeyer-Villiger biooxidation * cyclohexanone monoxygenase * immobilization * viable whole-cell biocatalyst Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering OBOR OECD: Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation Impact factor: 3.082, year: 2016 http://www.mdpi.com/2073-4344/7/11/353

  9. Study of viable models with non-universal gaugino mediation with CompHEP and ISAJET

    International Nuclear Information System (INIS)

    Baer, H.; Balazs, C.; Belyaev, A.; Dermisek, R.; Mafi, A.; Mustafayev, A.

    2003-01-01

    We study the recently proposed scenario for SUSY GUT models in which compactification of the extra dimension(s) leads to a breakdown of the gauge symmetry and/or supersymmetry. SUSY breaking occurs on a hidden brane, and is communicated to the visible brane via gaugino mediation. The non-universal gaugino masses are developed at the compactification scale as a consequence of a restricted gauge symmetry on the hidden brane. For gaugino masses related due to a Pati-Salam symmetry on the hidden brane, we find the limited, but significant, regions of the model parameter space where a viable spectra of SUSY matter is generated. In the more general case of three independent gaugino masses, large parameter space regions open up for large values of the U(1) gaugino mass M 1 . We also find the relic density of neutralinos for these models to be generally below the expectations from cosmological observations, thus leaving room for hidden sector states to make up the bulk of cold dark matter

  10. Drug delivery interfaces in the 21st century: from science fiction ideas to viable technologies.

    Science.gov (United States)

    Chertok, Beata; Webber, Matthew J; Succi, Marc D; Langer, Robert

    2013-10-07

    Early science fiction envisioned the future of drug delivery as targeted micrometer-scale submarines and "cyborg" body parts. Here we describe the progression of the field toward technologies that are now beginning to capture aspects of this early vision. Specifically, we focus on the two most prominent types of systems in drug delivery: the intravascular micro/nano drug carriers for delivery to the site of pathology and drug-loaded implantable devices that facilitate release with the predefined kinetics or in response to a specific cue. We discuss the unmet clinical needs that inspire these designs, the physiological factors that pose difficult challenges for their realization, and viable technologies that promise robust solutions. We also offer a perspective on where drug delivery may be in the next 50 years based on expected advances in material engineering and in the context of future diagnostics.

  11. Vermicomposting and anaerobic digestion – viable alternative options for terrestrial weed management – A review

    Directory of Open Access Journals (Sweden)

    Biswanath Saha

    2018-03-01

    Full Text Available The management of terrestrial weed is of great concern for the scientific community as these weeds cause adverse effect in different ecosystems like forest, agriculture and urban. The widespread of these weeds by their adaptive capability and morphological advancement is difficult to control. Parthenium hysterophorous, Lantana camara, Saccharum spontaneum, Ageratum conyzoides are the weeds that spread all over the world. There are various management practices employed for the control of this weeds. But all of these practices have some drawbacks those are neither environment friendly nor economical. In this paper a review has been done to evaluate various alternative management practices for these terrestrial weeds and to analyze their feasibility. Vermicomposting and anaerobic digestion can be viable alternative option which is cost effective as well. There are few studies regarding vermicomposting and anaerobic digestions of terrestrial weeds are done.

  12. Análise do custo e do raio econômico de transporte de madeira de reflorestamentos para diferentes tipos de veículos Cost and distance of reforestation wood transport for different types of trucks

    Directory of Open Access Journals (Sweden)

    Márcio Lopes da Silva

    2007-12-01

    Full Text Available Este estudo foi realizado com o objetivo de estabelecer a distância máxima de transporte viável para cada tipo de veículo utilizado para transportar a madeira das áreas de colheita até centros de consumo. Para tanto, utilizaram-se dados de custos e receitas de um reflorestamento, bem como a capacidade de carga de diferentes composições veiculares empregadas no transporte. Aplicando-se os critérios econômicos (VPL, TIR, CMP e BCPE, as distâncias variaram entre 155 e 226 km, para o caminhão-truck e o rodotrem, respectivamente. Concluiu-se que o rodotrem pode alcançar distância maior de transporte, sendo o preço da madeira a variável que mais influenciou a distância máxima de transporte.The objective of this study was to establish the viable transport maximum distance for each type of wood transportation vehicle from the harvest areas to the consumption centers. Thus, reforestation cost and revenue data were used, as well as the load capacity of different types of vehicles used for wood transport. The application of economic criteria (VPL, TIR, CMP and BCPE showed that the distances varied between 155 and 226 km, for the small truck and rodotrem (truck with two large trailers, respectively. It was concluded that rodotrem could reach a greater distance, with wood price being the factor most influencing maximum transport distance.

  13. First study on the formation and resuscitation of viable but nonculturable state and beer spoilage capability of Lactobacillus lindneri.

    Science.gov (United States)

    Liu, Junyan; Li, Lin; Li, Bing; Peters, Brian M; Deng, Yang; Xu, Zhenbo; Shirtliff, Mark E

    2017-06-01

    This study aimed to investigate the spoilage capability of Lactobacillus lindneri during the induction and resuscitation of viable but nonculturable (VBNC) state. L. lindneri strain was identified by sequencing the PCR product (amplifying 16S rRNA gene) using ABI Prism 377 DNA Sequencer. During the VBNC state induction by low temperature storage and beer adaption, total, culturable, and viable cells were assessed by acridine orange direct counting, plate counting, and Live/Dead BacLight bacterial viability kit, respectively. Organic acids and diacetyl concentration were measured by reversed-phase high performance liquid chromatography and head dpace gas chromatography, respectively. VBNC state of L. lindneri was successfully induced by both beer adaption and low temperature storage, and glycerol frozen stock was the optimal way to maintain the VBNC state. Addition of catalase was found to be an effective method for the resuscitation of VBNC L. lindneri cells. Furthermore, spoilage capability remained similar during the induction and resuscitation of VBNC L. lindneri. This is the first report of induction by low temperature storage and resuscitation of VBNC L. lindneri strain, as well as the first identification of spoilage capability of VBNC and resuscitated L. lindneri cells. This study indicated that the potential colonization of L. lindneri strain in brewery environment, formation and resuscitation of VBNC state, as well as maintenance in beer spoilage capability, may be an important risk factor for brewery environment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Prototipo autotérmico móvil para producción de biocarbón con biomasa de esquilmos de aguacate

    Directory of Open Access Journals (Sweden)

    Lenin Ejecatl Medina Orozco

    2018-04-01

    Full Text Available El estado de Michoacán, México, es el principal productor mundial de aguacate, con un impacto social y económico en función de la superficie cultivada. Productos secundarios de esta actividad, son los esquilmos agrícolas derivados de las podas de mantenimiento de los árboles. El biocarbón (biochar de subproductos de aguacate, se vislumbra como una alternativa económica, ambiental y socialmente viable, para producir biocarbón en grandes volúmenes, sin extracción de maderas de bosque y selvas prístinas. El objetivo del presente estudio, fue construir y evaluar el desempeño en condiciones de campo, de un prototipo de biocarbón-pirólisis autotérmico y móvil, con volumen útil de 1.7 m3 de biomasa triturada. El equipo fue construido de acero inoxidable, para darle un periodo de vida útil de 7 años bajo condiciones intensivas de uso, con movilidad dentro de los campos agrícolas y auto térmico para no depender de una fuente de calor externa. El equipo fue de bajo costo comparado con equipos comerciales ($ 700 000.00 pesos MN y con un potencial futuro en la cogeneración de energía. La principal variable en el costo de fabricación es el precio del acero inoxidable en el mercado. El equipo reportó una tasa de conversión de biomasa a biocarbón, en condiciones de campo, de entre 300 y 400 kg por día, con una tasa promedio de recuperación del 16% en biocarbón. Las principales variables en la transformación de la biomasa a biocarbón fueron: humedad, el tamaño de la astilla y condiciones atmosféricas. Se encontró una Capacidad de Intercambio Catiónico (CIC de 46 Cmolc kg-1 y pH 10.25. El equipo transformó satisfactoriamente la biomasa de aguacate y el biocarbón presentó características adecuadas para su uso en suelos volcánicos.

  15. TRABALHAR PARA ESTUDAR / ESTUDAR PARA TRABALHAR: REALIDADE E POSSIBILIDADES

    Directory of Open Access Journals (Sweden)

    Janes Siqueira

    2011-07-01

    Full Text Available O trabalho que apresentamos é parte da pesquisa com os estudantes das licenciaturas e da pedagogia da Universidade de Santa Cruz do Sul-UNISC-RS-Brasil. Este está inserido na linha de pesquisa: educação, trabalho e emancipação. Queremos compreender, à luz do materialismo histórico e dialético e das categorias da crítica da economia política, os significados atribuídos pelos estudantes universitários ao fenômeno trabalho e estudo. É uma pesquisa preferentemente qualitativa de natureza dialética. Para Marx, a essência da realidade humana reside no trabalho, mas a fonte de toda a riqueza está na natureza. Logo, trabalho, natureza e sociedade estão em relação dialética. Trabalho, portanto, é a ideia central articuladora, e natureza e sociedade devem ser estudadas em conexão com o trabalho. Marx trata o trabalho, no modo de produção capitalista, como impedimento ao desenvolvimento humano. Propõe o mesmo como um ato de criação e auto-expressão humana que não deve ter um valor. A compreensão dessa premissa é necessária para que os trabalhadores possam significar e ressignificar o trabalho para além da ideologia dominante. Algumas categorias aparecem como relevantes para a análise: condições de trabalho e de estudo, necessidade de formação, predominância do trabalho sobre o estudo, dificuldade de conciliar tempo de estudo e de trabalho. Na relação com o objeto de estudo, ressaltaremos a crise estrutural do capital, a desregulamentação das leis do trabalho e sua flexibilização e as contradições entre trabalho e estudo bem como quais possibilidades emancipatórias são visualizadas ou sonhadas pelos trabalhadores-estudantes. PALAVRAS-CHAVE: contradições entre trabalho e educação, condições e significados, realidade e possibilidades.

  16. Educación para la sostenibilidad

    Directory of Open Access Journals (Sweden)

    Educadores por la sostenibilidad

    2006-04-01

    Full Text Available La importancia dada por los expertos en sostenibilidad al papel de la educación queda reflejada en el lanzamiento mismo de la Década de la Educación para el Desarrollo Sostenible o, mejor, para un futuro sostenible (2005-2014 a cuyo impulso y desarrollo esta destinado este artículo, así como la página web que la Organización de Estados Iberoamericanos ha habilitado dentro de su portal digital, para la celebración de la Década.

  17. The comparison of nitroglycerin interventional dual-isotope myocardium perfusion imaging and 201Tl re-injection imaging to detect viable myocardium

    International Nuclear Information System (INIS)

    Gao Zhou; Shi yu; Chen Hongyan; Jia Shaowei

    2002-01-01

    Objective: Employing the differences in physical properties of 99m Tc-MIBI and 201 Tl, the authors discuss the contrast result of nitroglycerin interventional dual-isotope myocardium perfusion imaging and 201 Tl re-injection imaging to detect viable myocardium so that authors can enhance the image quality and shorten the examination time. Method: 34 OMI patients took the 99m Tc-MIBI and 201 Tl dual-isotope myocardium perfusion imaging and 201 Tl re-injection imaging respectively in two weeks. During the peak of normal dipyridamole stress i.v. 201 Tl 100 MBq was given and myocardium imaging was taken 15 min later. The dual-isotope group was given nitroglycerin 1mg under the tongue. Five min later, i.v. 99m Tc-MIBI 1110 mBq was given. In 201 Tl re-injection group i.v. 201 Tl 40 MBq was given 4 hour later and were imaged. Among the 34 OMI patients, 19 patients undertook another 99m Tc-MIBI static imaging. Results: There are no obvious differences between nitroglycerin interventional dual-isotope myocardium perfusion imaging and 201 Tl re-injection imaging in detection rate of viable myocardium, χ 2 =0.823, p>0.25. But they have great difference in perfusion changed sectional myocardium absorb rate, t=2.73, p 2 =27.867, p 201 Tl re-injection imaging

  18. Rapid quantification of viable Legionella in nuclear cooling tower waters using filter cultivation, fluorescent in situ hybridization and solid-phase cytometry.

    Science.gov (United States)

    Baudart, J; Guillaume, C; Mercier, A; Lebaron, P; Binet, M

    2015-05-01

    To develop a rapid and sensitive method to quantify viable Legionella spp. in cooling tower water samples. A rapid, culture-based method capable of quantifying as few as 600 Legionella microcolonies per litre within 2 days in industrial waters was developed. The method combines a short cultivation step of microcolonies on GVPC agar plate, specific detection of Legionella cells by a fluorescent in situ hybridization (FISH) approach, and a sensitive enumeration using a solid-phase cytometer. Following optimization of the cultivation conditions, the qualitative and quantitative performance of the method was assessed and the method was applied to 262 nuclear power plant cooling water samples. The performance of this method was in accordance with the culture method (NF-T 90-431) for Legionella enumeration. The rapid detection of viable Legionella in water is a major concern to the effective monitoring of this pathogenic bacterium in the main water sources involved in the transmission of legionellosis infection (Legionnaires' disease). The new method proposed here appears to be a robust, efficient and innovative means for rapidly quantifying cultivable Legionella in cooling tower water samples within 48 h. © 2015 The Society for Applied Microbiology.

  19. Obtención de ferromanganeso alto carbono y escoria para el desarrollo de materiales para soldar

    Directory of Open Access Journals (Sweden)

    Lorenzo Perdomo-González

    2000-03-01

    Full Text Available En el presente trabajo se realiza un análisis de la posibilidad de empleo de pirolusita en el desarrollo de aleaciones al manganeso y escorias con un sistema de óxidos MnO-SiO2-CaO con características propias para el desarrollo de fundentes para la soldadura automática para arco sumergido (SAAS. A partir de la composición química del mineral de manganeso se realizan los cálculos del proceso de reducción con el empleo de la tecnología sin fundente en presencia de coque como reductor, obteniéndose las adecuadas composiciones y las propiedades de los productos del proceso. Se realiza una corrida experimental de obtención de ferromanganeso con adiciones de dolomita, caolín y fluorita como correctores de la escoria. Se conforma una matriz de fundente para arco sumergido con la escoria y se le evalúan las propiedades tecnológicas de soldadura.

  20. Tumor-selective replication herpes simplex virus-based technology significantly improves clinical detection and prognostication of viable circulating tumor cells

    DEFF Research Database (Denmark)

    Zhang, Wen; Bao, Li; Yang, Shaoxing

    2016-01-01

    Detection of circulating tumor cells remains a significant challenge due to their vast physical and biological heterogeneity. We developed a cell-surface-marker-independent technology based on telomerase-specific, replication-selective oncolytic herpes-simplex-virus-1 that targets telomerase......-reverse-transcriptase-positive cancer cells and expresses green-fluorescent-protein that identifies viable CTCs from a broad spectrum of malignancies. Our method recovered 75.5-87.2% of tumor cells spiked into healthy donor blood, as validated by different methods, including single cell sequencing. CTCs were detected in 59-100% of 326...

  1. Preparation of a viable population of indium-111-labelled human blood platelets

    International Nuclear Information System (INIS)

    Heyns, A.; Badenhorst, P.N.; Pieters, H.; Loetter, M.G.; Minnaar, P.C.; Duyvene de Wit, L.J.; Reenen, O.R. van; Retief, F.P.; University of the Orange Free State, Bloemfontein; University of the Orange Free State, Bloemfontein; University of the Orange Free State, Bloemfontein

    1980-01-01

    Factors influencing labelling of human platelets with 111 Indium-8-hydroxyquinoline ([ 111 In]-oxine) in a physiological saline medium were investigated. The efficiency of labelling is influenced by time of incubation, concentration of oxine, and pH of the incubating medium. It was found that a viable platelet population could be labelled under the following conditions: (1) centrifugation of platelet rich plasma in polystyrene conical tubes at 800 g for 15 min; (2) resuspension of the platelet pellet in saline, pH 5.5; (3) incubating for 30 min at 22 0 C with [ 111 In]-oxine at a concentration of 6.25 mg oxine/litre platelet suspension; (4) washing once with platelet poor autologous plasma (PPP); and (5) finally suspending the platelets in PPP. The labelled platelets aggregated normally with collagen and ADP. Electron microscopy, done immediately after labelling, showed internal organelle reorganization characteristic of activated platelets. These ultrastructural features were reversible on incubationin PPP at 37 0 C for 30 min. The 111 In is not released from aggregated platelets and the label does not elute from incubated platelets for at least five hr. We conclude that human platelets thus labelled are suitable for in vivo kinetic studies. (orig.) [de

  2. Weeds as viable habitat for arthropod species in croplands of central Punjab

    International Nuclear Information System (INIS)

    Ruby, T.; Rana, S.A.; Rana, N.; Inayat, T.P.

    2011-01-01

    Weeds are considered a limiting factor of crop production. Simultaneously, these non-crop plants are a portion of the agricultural ecosystem and play an essential role as viable habitat for many organisms, including bio-control agents. Utilizing the quadrate method, sugarcane, fodder, wheat and mustard croplands were sampled for one year to determine the weed flora and arthropods living among it. Twenty weed species and eight major arthropod orders were found to be present. The majority of the weed plants were broad-leaved, while some were grass-like. A review of literature on Central Punjab weeds uncovered depicted a considerable change in the weed flora over few decades. This could be related to the intensive and extensive farming in the area, which has this increased over the few decades along with the construction of an extensive irrigation canal system. These alterations may have caused drastic changes in the soil structure and climate of the region. Most of the phytophagous arthropod species used weed plants as food. In turn, these were fed upon by a few zoophagous arthropod species that also utilized the weeds for shelter and oviposition. Thus, weeds have a specific role within the agro-ecosystem by supporting local biodiversity. (author)

  3. Evaluation of the BioVigilant IMD-A, a novel optical spectroscopy technology for the continuous and real-time environmental monitoring of viable and nonviable particles. Part II. Case studies in environmental monitoring during aseptic filling, intervention assessments, and glove integrity testing in manufacturing isolators.

    Science.gov (United States)

    Miller, Michael J; Walsh, Michael R; Shrake, Jerry L; Dukes, Randall E; Hill, Daniel B

    2009-01-01

    This paper describes the use of the BioVigilant IMD-A, a real-time and continuous monitoring technology based on optical spectroscopy, to simultaneously and instantaneously detect, size, and enumerate both viable and nonviable particles in a variety of filling and transfer isolator environments during an aseptic fill, transfer of sterilized components, and filling interventions. Continuous monitoring of three separate isolators for more than 16 h and representing more than 28 m3 of air per isolator (under static conditions) yielded a mean viable particle count of zero (0) per cubic meter. Although the mean count per cubic meter was zero, the detection of very low levels of single viable particles was randomly observed in each of these sampling runs. No viable particles were detected during the manual transfer of sterilized components from transfer isolators into a filling isolator, and similar results were observed during an aseptic fill, a filling needle change-out procedure, and during disassembly, movement, and reassembly of a vibrating stopper bowl. During the continuous monitoring of a sample transfer port and a simulated mousehole, no viable particles were detected; however, when the sampling probe was inserted beyond the isolator-room interface, the IMD-A instantaneously detected and enumerated both viable and nonviable particles originating from the surrounding room. Data from glove pinhole studies showed no viable particles being observed, although significant viable particles were immediately detected when the gloves were removed and a bare hand was allowed to introduce microorganisms into the isolator. The IMD-A technology offers the industry an unprecedented advantage over growth-based bioaerosol samplers for monitoring the state of microbiological control in pharmaceutical manufacturing environments, and represents significant progress toward the acceptance of microbiology process analytical technology solutions for the industry.

  4. Avaliação das competências de professores de enfermagem para desenvolver programas educativos para adultos

    Directory of Open Access Journals (Sweden)

    Patricia Bover Draganov

    2013-08-01

    Full Text Available Avaliar o desempenho que o enfermeiro docente de enfermagem atribui a si, nas competências para desenvolver programas educativos para adultos, foi objetivo desse estudo descritivo, comparativo, transversal e quantitativo, desenvolvido com 226 docentes enfermeiros de cursos de graduação em enfermagem paulistanos. Os dados foram colhidos por escala de Likert, lançados em planilha Excel® e analisados por estatística descritiva e testes não paramétricos de Wilcoxon e Friedman. A população tinha predominantemente entre 46-55 anos (87/38,49%, eram mulheres (198/87, 61% e mestres (180/79, 65%. Os professores consideraram-se distantes das competências que almejavam alcançar. A menor habilidade esteve em construir desenhos de programas para atender às situações de aprendizagem e a maior, em avaliar programas de ensino. O maior desejo esteve em desenhar programas com formatos criativos e o menor desejo para usar conselhos, comitês e força-tarefa. Concluiuse que desenhar programas educativos para adultos ainda é algo pouco explorado e pouco experenciado por esses docentes.

  5. Caminhos para a gestão integrada do território no Vale do Paraíba de 1950 a 2004

    Directory of Open Access Journals (Sweden)

    José Luiz Ronca Caruso

    2004-01-01

    Full Text Available The Paraíba Valley region and its political-administrative frame. The region and its two phases of Planning: the economic development and the sustainable one. The Paraíba Valley Road System as a policy of the economic planning. The Paraíba Valley Region and its physical conformation: the Paraíba do Sul river basin. The water resources management in the Paraíba do Sul river basin. Two levels of management: the federal and the one of the State. The water resources Planning and its possibilities for a sustainable development of the region, through a comprehensive management

  6. Universidades accesibles para todos

    Directory of Open Access Journals (Sweden)

    Roxana Stupp Kupiec

    2001-01-01

    Full Text Available Este trabajo pretende hacer conciencia sobrelos factores que inciden en la construcción colectiva deuniversidades dispuestas a innovar y transformar suideología, sus políticas, el currículum, los servicios estu-diantiles, la infraestructura física y administrativa y lasestrategias de comunicación e información, bajo el para-digma de la accesibilidad en el entorno. Se hace referen-cia al marco legal internacional y nacional que orientalas acciones que deben planificarse e implementarse enlas instituciones de Educación Superior.En la construcción de universidades accesibles para to-dos se requiere de un cambio en la concepción curricu-lar. Se analizan conceptos como la flexibilidad en la for-mación y el acceso a los planes de estudio para respondera las necesidades educativas de todos los estudiantes. Para promover estas transformaciones se proponen prin-cipios y prácticas tales como la interdisciplinareidad y lainclusión del tema de la discapacidad en todas las carre-ras. Además, se presentan ejemplos de aplicación en dife-rentes áreas del perfil profesional, en la formación deldocente universitario y en actividades de proyección so-cial, vida estudiantil e investigación. En el ámbito estu-diantil, se describen algunos servicios y procesos que sehan desarrollado en la Universidad de Costa Rica

  7. Propuesta para el cálculo del Índice Dm para la validez de los ítemes

    OpenAIRE

    Dominguez Lara, Sergio

    2013-01-01

    Se presenta un programa en formato MS Excel para calcular el índice Dm para la validez de los ítemes. La validez es un proceso de acumulación de pruebas para apoyar la interpretación y el uso de las puntuaciones de los tests psicológicos, siendo las relaciones de los puntajes con otras variables externas una importante fuente de validación. Una variante es la llamada validez relacionada al criterio, en la cual se espera una correlación significativa con aquellos criterios externos que son teó...

  8. Educación para el Ocio: Ha llegado la hora de un nuevo objetivo para una vieja idea.

    Directory of Open Access Journals (Sweden)

    Susan Hutchinson

    2012-01-01

    Full Text Available La Educación para el Ocio nos sitúa ante una vieja idea, que está siendo examinada con renovado vigor en Canadá. Profesionales e investigadores de diversas disciplinas (por ejemplo, en el ámbito de Salud, la Justicia, la Educación o la Recreación reconocen, cada vez más, que son muchas las personas que pueden beneficiarse del ocio y de las oportunidades de recreación en sus comunidades, aún cuando son bastantes las no pueden hacerlo porque carecen de alfabetización en ocio. Esto es, de los conocimientos, habilidades y confianza que se necesitan para darle un significado personal, que incida positivamente en su bienestar y calidad de vida. La Educación para el Ocio es un medio clave para mejorar la alfabetización en ocio. El texto presenta un modelo de educación para el ocio desarrollado por la Dra. Brenda Robertson, argumentando cómo y por qué la Educación para el Ocio es necesaria para abordar la salud mental y el bienestar de las personas que sufren marginación en sus comunidades. Complementariamente se presentan algunas recomendaciones para avanzar esta Educación, como un componente fundamental de la agenda nacional para la recreación en Canadá.--------------------------------------------------------------------Leisure education is an old idea that is being examined with renewed vigour in Canada. Practitioners and researchers from various disciplines (e.g., health, justice, education, recreation are recognising anew that many people who could benefit from leisure or recreation opportunities in their communities are unable to do so because they lack leisure literacy. Leisure literacy refers to the knowledge, skills and confidence to engage in personally meaningful, health-enhancing leisure. Leisure education is a key means to enhance leisure literacy. The paper presents a model of leisure education developed by Dr. Brenda Robertson and argues for how and why leisure education is needed to address the mental health and well

  9. O efeito trabalhador adicional para filhos no Brasil

    Directory of Open Access Journals (Sweden)

    Elzira Lúcia de Oliveira

    2014-06-01

    Full Text Available O objetivo deste trabalho é testar a hipótese da existência do efeito trabalhador adicional para filhos no Brasil, procurando identificar se a situação de desemprego do chefe de família fará com que algum membro da família, cuja condição seja filho, transite para a População Economicamente Ativa - PEA. A base de dados utilizada foi a Pesquisa Mensal de Emprego - PME realizada pelo IBGE, que permite a construção de painéis para análise longitudinal de dados. A hipótese foi testada para pelo menos um filho com idade de 10 a 18 anos, entre 2002 e 2013, para as regiões metropolitanas de Belo Horizonte, Salvador, Porto Alegre, São Paulo, Rio de Janeiro e Recife, que compõem a área de abrangência da PME. Este trabalho admite a existência de diferencial por sexo do chefe para o efeito trabalhador adicional de filhos de 10 a 18 anos. Os resultados mostraram haver um efeito positivo maior para chefes homens do que para chefes mulheres, sendo que a variável de transição do filho para a atividade não apresentou significância estatística que permitisse assumir a existência do efeito. Corroborou-se a hipótese da existência de diferencial por sexo, contudo no sentido oposto ao da hipótese assumida.

  10. The Independent Evolution Method Is Not a Viable Phylogenetic Comparative Method.

    Directory of Open Access Journals (Sweden)

    Randi H Griffin

    Full Text Available Phylogenetic comparative methods (PCMs use data on species traits and phylogenetic relationships to shed light on evolutionary questions. Recently, Smaers and Vinicius suggested a new PCM, Independent Evolution (IE, which purportedly employs a novel model of evolution based on Felsenstein's Adaptive Peak Model. The authors found that IE improves upon previous PCMs by producing more accurate estimates of ancestral states, as well as separate estimates of evolutionary rates for each branch of a phylogenetic tree. Here, we document substantial theoretical and computational issues with IE. When data are simulated under a simple Brownian motion model of evolution, IE produces severely biased estimates of ancestral states and changes along individual branches. We show that these branch-specific changes are essentially ancestor-descendant or "directional" contrasts, and draw parallels between IE and previous PCMs such as "minimum evolution". Additionally, while comparisons of branch-specific changes between variables have been interpreted as reflecting the relative strength of selection on those traits, we demonstrate through simulations that regressing IE estimated branch-specific changes against one another gives a biased estimate of the scaling relationship between these variables, and provides no advantages or insights beyond established PCMs such as phylogenetically independent contrasts. In light of our findings, we discuss the results of previous papers that employed IE. We conclude that Independent Evolution is not a viable PCM, and should not be used in comparative analyses.

  11. Biocomercio: una alternativa para el desarrollo sostenible

    Directory of Open Access Journals (Sweden)

    Carlos Murillo R.

    2008-01-01

    Full Text Available El desarrollo sostenible en países ricos en biodiversidad dependerá de la capacidad de los gobiernos de implementar los arreglos institucionales necesarios para conciliar la preservación de los recursos naturales con el uso de los mismos para la producción de bienes y servicios que puedan potenciar el crecimiento económico y un mayor bienestar para las comunidades locales donde estos recursos se encuentren. Este es el objetivo de la Iniciativa de Biocomercio que ha venido impulsando la Conferencia de Naciones Unidas para el Comercio y el Desarrollo (UNCTAD en distintos países de Latinoamérica. En el tanto estos principios y los establecidos en el CDB se puedan viabilizar e inspiren los distintos Programas Nacionales de Biocomercio en los países de la región, parecería no haber dudas de que la Iniciativa abre nuevas puertas para el uso, aprovechamiento y desarrollo sostenible de los mismos.

  12. ANÁLISE, MODELAGEM E IMPRESSÃO 3D DE UM FRAME PARA VANTs DO TIPO QUADRÍCOPTEROS PARA VOOS EM AMBIENTE INDOOR

    Directory of Open Access Journals (Sweden)

    Rafael Rocha Gomes

    2017-01-01

    Full Text Available O foco do projeto de pesquisa foi analisar, modelar e construir utilizando uma impressora 3D, um frame de um Veículo Aéreo Não Tripulado (VANT para voos em ambiente indoor. Esta aeronave será usada para experimentos laboratoriais, que exijam características especiais para ambientes indoor e de pesquisa, tais como, ser compacto, leve e uma estrutura que minimize os riscos aos operadores, pois dividirá espaço com pessoas. A motivação desta pesquisa se deu pelo fato dos frames disponíveis no mercado serem voltados para voos outdoor, como por exemplo, os utilizados para monitoramento. Com o intuito de proporcionar uma solução de aeronave para espaço laboratorial indoor, a proposta deste trabalho foi de modelar e imprimir em 3D um protótipo de frame que atenda todos os requisitos necessários para este tipo de voo.

  13. Para una Mejor Comprensión del Desarrollo: Análisis conceptual, lógica para su abordaje e instrumentos para su medición

    Directory of Open Access Journals (Sweden)

    Héctor Cárcamo

    2006-01-01

    cross nacional para la realización de estudios vinculados al desarrollo. Se plantea una revisión de conceptos fundamentales como el de desarrollo y la necesidad de la equivalencia funcional. El documento continúa con una reflexión guiada desde la noción de desarrollo social; asumiendo críticamente que el desarrollo se ha plasmado como un intento por lograr una radiografía del todo que ha olvidado la integración de sus partes constituyentes, negando de esta manera la diversidad como condición esencial para dar sentido a la idea de una visión integradora. Finalmente se realiza una revisión de CASEN como instrumento para medir el desarrollo social en Chile.

  14. ¿ES VIABLE EL CONDUCTISMO EN EL SIGLO XXI? / IS BEHAVIORISM VIABLE IN THE XXI CENTURY?

    Directory of Open Access Journals (Sweden)

    Telmo Eduardo Peña Correal*

    2010-08-01

    Full Text Available RESUMENSe hace una revisión conceptual sobre el conductismo en la cual se discuten sus principales características, con elobjetivo de determinar su viabilidad como propuesta para la psicología del siglo XXI. A partir del supuesto de que elconductismo no es una teoría unificada de la psicología, sino una filosofía de esta disciplina, se presentan los tópicos sobrelos cuales hay mayor coincidencia entre los conductistas, a saber, la idea de que la psicología es una ciencia natural, que esuna ciencia empírica, que intenta generar un conocimiento en el que se controlen los sesgos subjetivistas (objetividad yfinalmente que utiliza en la tarea investigativa de forma preferente la metodología experimental. Se señalan igualmentealgunos tópicos sobre los cuales hay debate entre los conductistas, tales como el nivel de molecularidad o molaridad en elanálisis, la necesidad o no de factores mediadores y el uso de la investigación ideográfica vs la investigación nomotética.El trabajo concluye señalando cómo algunos de los conceptos más conocidos como «estímulo», «respuesta»,«condicionamiento» entre otros, no son esenciales al conductismo y eventualmente podrían ser reemplazados por otros.ABSTRACTA conceptual review of Behaviorism is presented in which its main features are discussed with the purpose of assessingits viability as a proposal for the XXIth century. Based on the premise that behaviorism is not a unified theory of psychology,but a philosophy of this science, some of the topics on which there is greater coincidence between the behaviourists arepresented, namely the assumption that psychology is a natural and empirical science, which attempts to generate knowledgein which subjectivistic biases (objectivity are controlled and finally that it prefers the experimental methodology in theirempirical studies. Also are brought some topics on which there is debate among the behaviorists, such as the level ofmolecularity or

  15. GOOGLE DRIVE: POTENCIALIDADES PARA O DESIGN DE MATERIAL EDUCACIONAL DIGITAL (MED PARA ENSINO DE LÍNGUAS

    Directory of Open Access Journals (Sweden)

    Rosinda de Castro Guerra RAMOS

    2017-07-01

    Full Text Available Este trabalho tem como objetivo apresentar uma experiência sobre como o Google Drive pode ser usado para o desenho de MED (Material Educacional Digital para o ensino-aprendizagem de línguas, devido ao crescente uso das TDICs (Tecnologias Digitais de Informação e Comunicação na área de educação e aos desafios que o design de materiais educacionais para o ambiente digital nos lançam.  Para apresentar nossa experiência, faremos uma discussão teórica sobre os conceitos de desenvolvimento de material didático (TOMLINSON, 2003; RAMOS, 2009, de material educacional digital –MED – (BEHAR, 2009, multimodalidade (ROJO e BARBOSA, 2015, a lacuna existente em relação ao  letramento digital na formação de professores (LEFFA, 2013, os níveis/tipos  de interação em ambiente digital (HIRUMI, 2013 e sua relação na coconstrução de novos conhecimentos (VIGOTSKY, 1989, 1998; OLIVEIRA, 1997; BRASIL, 1998. Em seguida, descreveremos o Google Drive e as funcionalidades dos aplicativos de produtividade Google Documentos, Planilhas, Apresentações e Formulários. Finalizaremos com considerações sobre essa experiência e suas contribuições para a área de formação de professores de línguas.

  16. Vaccines against drugs of abuse: a viable treatment option?

    Science.gov (United States)

    Kantak, Kathleen M

    2003-01-01

    Drug addiction is a chronically relapsing brain disorder. There is an urgent need for new treatment options for this disease because the relapse rate among drug abusers seeking treatment is quite high. During the past decade, many groups have explored the feasibility of using vaccines directed against drugs of abuse as a means of eliminating illicit drug use as well as drug overdose and neurotoxicity. Vaccines work by inducing drug-specific antibodies in the bloodstream that bind to the drug of abuse and prevent its entry into the brain. The majority of work in this area has been conducted with vaccines and antibodies directed against cocaine and nicotine. On the basis of preclinical work, vaccines for cocaine and nicotine are now in clinical trials because they can offer long-term protection with minimal treatment compliance. In addition, vaccines and antibodies for phencyclidine, methamphetamine and heroin abuse are currently under development. An underlying theme in this research is the need for high concentrations of circulating drug-specific antibodies to reduce drug-seeking and drug-taking behaviour when the drug is repeatedly available, especially in high doses. Although vaccines against drugs of abuse may become a viable treatment option, there are several drawbacks that need to be considered. These include: a lack of protection against a structurally dissimilar drug that produces the same effects as the drug of choice;a lack of an effect on drug craving that predisposes an addict to relapse; and tremendous individual variability in antibody formation. Forced or coerced vaccination is not likely to work from a scientific perspective, and also carries serious legal and ethical concerns. All things considered, vaccination against a drug of abuse is likely to work best with individuals who are highly motivated to quit using drugs altogether and as part of a comprehensive treatment programme. As such, the medical treatment of drug abuse will not be radically

  17. Factores de riesgo para el neonato pequeño para la edad gestacional en un hospital de Lima, Perú

    Directory of Open Access Journals (Sweden)

    J. Eduardo Tejeda-Mariaca

    Full Text Available Objetivos. Identificar factores de riesgo para neonatos a término pequeños para la edad gestacional. Materiales y métodos. Cohorte retrospectiva que utilizó datos del Sistema Informático Materno Perinatal del Hospital María Auxiliadora de Lima, del período 2000 a 2010. Se evaluó la edad materna, paridad, nivel educativo, estado civil, índice de masa corporal pregestacional, número de controles prenatales, presencia de patologías como preeclampsia, eclampsia, infección urinaria y diabetes gestacional como factores de riesgo en pequeños para edad gestacional. El peso para la edad gestacional fue calculado sobre la base de percentiles peruanos. Se calcularon los riesgos relativos crudos (RR y ajustados (RRa con sus intervalos de confianza al 95% usando modelos lineales generalizados log binomial. Resultados. Se incluyeron 64 670 gestantes. La incidencia de pequeños para la edad gestacional fue 7,2%. La preeclampsia (RRa 2,0; IC 95%: 1,86-2,15, eclampsia (RRa 3,22; IC 95%: 2,38-4,35, bajo peso materno (RRa 1,38; IC 95%: 1,23-1,54, nuliparidad (RRa 1,32; IC 95%: 1,23-1,42, edad ≥35 años (RRa 1,16; IC 95%: 1,04-1,29, tener controles prenatales de 0 a 2 (RRa 1,43; IC 95%: 1,32-1,55 y 3 a 5 (RRa 1,22; IC 95%: 1,14-1,32 fueron factores de riesgo para ser pequeños para la edad gestacional. Conclusiones. Es necesario identificar a las gestantes con factores de riesgo como los encontrados para disminuir la condición de pequeños para la edad gestacional. Se debe actuar poniendo énfasis en factores modificables, tales como la frecuencia de sus controles prenatales

  18. Ciclo Hápticos: Para Quinteto de Sopros

    Directory of Open Access Journals (Sweden)

    Jorge Luiz de Lima Santos

    2016-06-01

    Full Text Available Memorial e partitura de Ciclo Hápticos, escrita em 2014 para quinteto de sopros. O memorial descreve brevemente as ferramentas metodológicas empregadas para o planejamento e criação da obra. De um lado, para produção do material melódico-harmônico foi empregado o Sistema-Gr de composição musical, desenvolvido por Carlos Almada (2014. Do outro lado, para organização formal, utilizamos o software Partitions - que faz parte do conjunto de programas do Parsemat, elaborado por Pauxy Gentil-Nunes (2009 – o qual informa o conjunto total de possibilidades de partições de um dado número.

  19. Retinal Electrophysiology Is a Viable Preclinical Biomarker for Drug Penetrance into the Central Nervous System

    Directory of Open Access Journals (Sweden)

    Jason Charng

    2016-01-01

    Full Text Available Objective. To examine whether retinal electrophysiology is a useful surrogate marker of drug penetrance into the central nervous system (CNS. Materials and Methods. Brain and retinal electrophysiology were assessed with full-field visually evoked potentials and electroretinograms in conscious and anaesthetised rats following systemic or local administrations of centrally penetrant (muscimol or nonpenetrant (isoguvacine compounds. Results. Local injections into the eye/brain bypassed the blood neural barriers and produced changes in retinal/brain responses for both drugs. In conscious animals, systemic administration of muscimol resulted in retinal and brain biopotential changes, whereas systemic delivery of isoguvacine did not. General anaesthesia confounded these outcomes. Conclusions. Retinal electrophysiology, when recorded in conscious animals, shows promise as a viable biomarker of drug penetration into the CNS. In contrast, when conducted under anaesthetised conditions confounds can be induced in both cortical and retinal electrophysiological recordings.

  20. Políticas macroeconómicas, deterioro en el bienestar social y la viabilidad de las Pymes y empresas de economía social para incentivar el desarrollo económico local : un análisis empírico del caso mexicano (1983-2012)

    OpenAIRE

    Mora Heredia, Alfredo

    2013-01-01

    En esta investigación se presentan varios análisis empíricos (cuantitativos) para identificar los efectos de la estrategia económica implementada en México, desde 1983 hasta la fecha, en la reducción de los mínimos de bienestar en la población mexicana. También se propone como estrategia viable incentivar el desarrollo económico desde los ámbitos locales, dando prioridad a la creación de Micro, Pequeña y Mediana empresa (MiPyMIE) y empresas de economía social en los ámbitos regionales más...

  1. Palladium-mediated conversion of para-aminoarylboronic esters into para-aminoaryl- 11C-methanes

    DEFF Research Database (Denmark)

    Andersen, Valdemar Lykke; Herth, Matthias Manfred; Lehel, Szabolcs

    2013-01-01

    of free amines. Aryl boronic esters containing primary, secondary, and tertiary amines were successfully converted into corresponding labeled methyl derivatives in suf¿cient radiochemical yield to apply this method for tracer development. This procedure was applied to the labeling of CIMBI-712......Cross-couplings are an alternative to conventional 11C-methylations which are generally employed in PET tracer synthesis. Therefore, we set out to develop a general procedure for the synthesis of para-11CH3 labeled aromatic amines from the corresponding para-aminoarylboronic esters in the presence...

  2. Sistema de autenticidad para aplicaciones de análisis de eventos para seguridad.

    OpenAIRE

    ESCAMILLA PARDO, TONI

    2017-01-01

    [ES] En este proyecto se afronta la creación de un sistema distribuido capaz de garantizar, en aplicaciones empleadas para el análisis de eventos de seguridad, la autenticidad de los datos transmitidos entre el host cliente y servidor con el fin de asegurar la integridad de los mismos en la transmisión entre ambas partes, así como evitar la posible alteración en el destino. Para ello, se utilizará la tecnología blockchain a modo de base de datos en la que se almacenen los hashe...

  3. La medición y vigilancias de la enfermedad de la mancha foliar producida por la sigatoka negra (Mycospharella fijiensis var diformis Morelet en la producción de musáceas: un enfoque para lograr la sostenibilidad del desarrollo

    Directory of Open Access Journals (Sweden)

    Edgardo Vargas Jarquín

    2013-05-01

    Full Text Available En el presente trabajo se discute la necesidad de utilizar tecnología, como el procesamiento digital de imágenes para mejorar los procesos de medición y vigilancia de la enfermedad de la mancha de la hoja causada por SIGATOKA NEGRA (Mycospharella fijiensis var diformis Morelet, pero partiendo de un análisis epistemológico que fundamenta al desarrollo sostenible, que es el marco dentro del cual se encuentra sentido a la aplicación de esta tecnología. Así se define que es la gobernabilidad benéfica del ambiente lo que dirige la gestión de los diferentes actores y esta se da en virtud de comprender, mantener, proteger y administrar la complejidad ambiental.  Se señala como uno de los principales inconvenientes de la producción de musáceas la enfermedad de la sigatoka negra, pues produce costos de producción de aproximadamente 1.300 dólares al año costo en uso y aplicación de pesticidas.  Se discute sobre el enfoque de los trabajos de investigación para el desarrollo sostenible en el cultivo de las musáceas.  Se presentan dos experiencias para medir y vigilar la sigatoka negra. En un caso se desarrolla un sistema convencional denominado SiMu, que permite utilizar medios computacionales asistidos para, sobre todo, medir y vigilar la incidencia de la sigatoka. En el otro caso se utiliza tecnología de procesamiento digital de imágenes para realizar ese mismo trabajo pero con un mayor nivel de automatización.  Queda planteada la interrogante de si esta tecnología se puede usar para pequeños y medianos productores, tanto de banano como de plátano, puesto que en ese tipo de unidades no se podría tener una forma económicamente viable para obtener imágenes digitales aéreas.

  4. Bio sorption of Reactive Dye from Textile Wastewater by Non-viable Biomass of Aspergillus niger and Spirogyra sp

    International Nuclear Information System (INIS)

    Khalaf, M.A.

    2008-01-01

    The Potential of Aspergillus niger fungus and Spirogyra sp., a fresh water green algae, was investigated as a bio sorbents for removal of reactive dye (Synazol) from its multi-component textile wastewater. Pre-treatment of fungal and algal biomass with autoclaving increased the removal of dye more than that pre-treated with gamma-irradiation. The heat dried autoclaved biomass for the 2 organisms exhibited maximum dye removal at ph 3, temperature 30 degree C and 8 g/l (w/v) biomass conc. after 18 h contact time. The results showed that the non-viable biomass possessed high stability and efficiency of dye removal over 3 repeated batches

  5. Para uma abordagem virtuosa do Jornalismo

    Directory of Open Access Journals (Sweden)

    Rogério Christofoletti

    2012-06-01

    Full Text Available As últimas décadas têm favorecido o crescimento do interesse e dos estudos sobre a ética jornalística. No Brasil e em outras praças, as abordagens deste tema têm sido na maioria das vezes por um caráter deontológico ou utilitarista. Neste artigo, chamamos a atenção para uma perspectiva que privilegie as virtudes como pontos de partida para os debates acerca das condutas dos profissionais. Uma ética das virtudes remete a Aristóteles e aos estoicos, mas vem sendo retomada por autores mais contemporâneos, como Macintyre e Comte-Sponville, entre outros. No jornalismo, esta abordagem não é definitiva ou totalizante, mas contribui para uma compreensão mais ampla da discussão, chamando inclusive a atenção para recomendações éticas úteis, práticas e atuais.

  6. Educación para la Salud: espacio curricular optativo para los estudiantes de la carrera de Medicina

    OpenAIRE

    Susacasa, Sandra; Candreva, Anna; Cerasa, Silvana; Azanza, Marisa

    2016-01-01

    La educación para la salud adquiere relevancia en los programas institucionales internacionales. Estos se orientan a promover un mayor nivel de salud en la población. Sin embargo, las problemáticas vinculadas al campo de la educación para la salud, de acuerdo con los informes epidemiológicos, no están mejorando. Aún más, la situación se vuelve más crítica. En nuestro país las demandas sociales expresan la urgente necesidad de instalar la Educación para la Salud en espacios educativos y de sal...

  7. Modelo para la implementación de controles internos para empresas constructoras.

    OpenAIRE

    Choez Chiquito, Wilton Antonio

    2015-01-01

    La presente investigación se refiere al tema Metodología para la implementación de nuevos métodos de Control para las empresas de construcción en el Ecuador. Donde las características principales de este sector ha sido su rápido crecimiento en las últimas dos décadas, con un alto número de trabajadores de obras con bajo o ningún nivel de educación, falta de compromiso por parte de los empleados de obra por el alto nivel de rotación que puede existir debido a que las construcciones son por per...

  8. ELEMENTOS PARA LA CONSTRUCION DE UN PERFIL PARA EL GERENTE SOCIAL EN UN ENTORNO GLOBALIZADO

    Directory of Open Access Journals (Sweden)

    Antonio Bastidas Unigarro

    2009-07-01

    Full Text Available El perfil del gerente social adecuado para enfrentar los retos del mundo globalizado se ha estructurado a partir de cuatro requerimientos fundamentales: los conocimientos, la experiencia, las habilidades y las actitudes, los cuales se analizan como competencias indispensables para administrar la problemática compleja, llena de incertidumbres y desafíos del nuevo milenio. La propuesta para la construcción de un perfil de este gerente parte de considerar tres tipos de competencias: las funcionales, las comportamentales y los requisitos de estudio y experiencia. Su construcción parte del análisis de la Gerencia Social en el estado neoliberal, escenario, que reclama la formación de gerentes sociales comprometidos en la construcción de una sociedad mas justa, incluyente, participativa, democrática y solidaria.

  9. Study of distribution of /sup 169/Yb, /sup 67/Ga and /sup 111/In in tumor tissue by macroautoradiography. Comparison between viable tumor tissue and necrotic tumor tissue

    Energy Technology Data Exchange (ETDEWEB)

    Ando, A; Sanada, S; Hiraki, T [Kanazawa Univ. (Japan). School of Paramedicine; Doishita, K; Ando, I

    1977-01-01

    The localization of /sup 169/Yb, /sup 67/Ga and /sup 111/In in tumor tissues was determined macroautoradiographically. /sup 169/Yb-citrate, /sup 67/Ga-citrate and /sup 111/In-citrate were injected intravenously into rats which had received subcutaneously transplantations of Yoshida sarcoma, and were injected intraperitoneally to the mice which had received subcutaneous transplantations of Ehrlich tumor. These animals were sacrificed 3, 24 and 48 hours after injection. The tumor tissues were frozen in n-hexane (-70/sup 0/C) cooled with dry ice-acetone. After this, the frozen tumor tissues were cut into thin serial sections (10 ..mu..m) in a cryostat (-20/sup 0/C). One of these sections was then placed on x-ray film, and this film was developed after exposure of several days. The next slice of each of these sections were stained using the hematoxylin and eosin. From the observations of these autoradiogram and H-E stained slice, the following results were obtained. Concentration of /sup 169/Yb, /sup 67/Ga and /sup 111/In was predominant in viable tumor tissue rather than in necrotic tumor tissue, regardless of time after administration. /sup 67/Ga and /sup 111/In were distributed uniformly in viable tumor tissue, but there was greater deposition of /sup 169/Yb in viable tumor tissue neighboring the necrotic tumor.

  10. DEVELOPMENT OF A WELDING PROCEDURE FOR MIL A 46100 ARMOR STEEL JOINTS USING GAS METAL ARC WELDING

    OpenAIRE

    DAVID MAZUERA ROBLEDO; JOHN ALBERTO SUÁREZ GÓMEZ; JORGE ENRIQUE GIRALDO BARRADA

    2011-01-01

    Se desarrolló un procedimiento de soldadura (WPS) para unir láminas de acero para blindaje bajo especificación MIL A46100 usando el proceso GMAW, el cual fue calificado mediante ensayos mecánicos y no destructivos (NDT) de acuerdo con los requerimientos de códigos militares. Los resultados obtenidos fueron comparados con procedimientos de soldadura para este material realizados con el proceso SMAW. Los resultados obtenidos mostraron que el WPS diseñado es una opción viable para la soldadura d...

  11. Nuevo lenguaje para un nuevo medio

    Directory of Open Access Journals (Sweden)

    Inma Martín Herrera

    2014-12-01

    Full Text Available El concepto tradicional de comunicación unidereccional se ha roto para convertirse en comunicación pluridireccional que permitirá la retroalimentación informativa. Las nuevas formas de redactar, contar diseñar y presentar la información en Internet exige nuevos hábitos de lectura por parte de la audiencia. El lector deberá tomar conciencia de que está frente a un medio diferente con el que debe familiarizarse para hacer uso correcto del soporte digital, se informará adecuadamente para disfrutar de las ventajas del innovador medio, conocido como Periodismo de servicios o Nuevo Periodismo.

  12. “Libros para niñas y libros para niños”: Presencia de estereotipos de género en una colección de libros para dibujar

    Directory of Open Access Journals (Sweden)

    Yolanda Cabrera García-Ochoa

    2014-06-01

    Full Text Available Los aprendizajes que se producen a partir de las propuestas imaginarias y simbólicas en los materiales didácticos pueden contener elementos sexistas. El objetivo del estudio consiste en determinar, a través de un análisis de contenido, si la colección de libros para dibujar “Doodle para niñas” y “Doodle para niños” representa roles estereotipados de género en sus imágenes y en el texto escrito. Tanto a nivel de representación gráfica como escrita, ambos libros utilizan roles estereotipados sobre todo en detrimento de la mujer. Aunque el sistema educativo español señala hace años un modelo igualitario para eliminar la discriminación, los avances conseguidos desde un prisma constitucional y legal, van muy por delante de unos sistemas culturales y sociales que apenas han evolucionado.

  13. Molecular characterization of viable Legionella spp. in cooling tower water samples by combined use of ethidium monoazide and PCR.

    Science.gov (United States)

    Inoue, Hiroaki; Fujimura, Reiko; Agata, Kunio; Ohta, Hiroyuki

    2015-01-01

    Viable Legionella spp. in environmental water samples were characterized phylogenetically by a clone library analysis combining the use of ethidium monoazide and quantitative PCR. To examine the diversity of Legionella spp., six cooling tower water samples and three bath water samples were collected and analyzed. A total of 617 clones were analyzed for their 16S rRNA gene sequences and classified into 99 operational taxonomic units (OTUs). The majority of OTUs were not clustered with currently described Legionella spp., suggesting the wide diversity of not-yet-cultured Legionella groups harbored in cooling tower water environments.

  14. Acanthamoeba polyphaga resuscitates viable non-culturable Legionella pneumophila after disinfection.

    Science.gov (United States)

    García, María Teresa; Jones, Snake; Pelaz, Carmen; Millar, Richard D; Abu Kwaik, Yousef

    2007-05-01

    Amoebae are the natural hosts for Legionella pneumophila and play essential roles in bacterial ecology and infectivity to humans. When L. pneumophila colonizes an aquatic installation, it can persist for years despite repeated treatments with disinfectants. We hypothesized that freshwater amoebae play an important role in bacterial resistance to disinfectants, and in subsequent resuscitation of viable non-culturable (VNC) L. pneumophila that results in re-emergence of the disease-causing strain in the disinfected water source. Our work showed that in the absence of Acanthamoeba polyphaga, seven L. pneumophila strains became non-culturable after treatment by 256 p.p.m. of sodium hypochlorite (NaOCl). In contrast, intracellular L. pneumophila within A. polyphaga was resistant to 1024 p.p.m. of NaOCl. In addition, L. pneumophila-infected A. polyphaga exhibited increased resistance to NaOCl. When chlorine-sterilized water samples were co-cultured with A. polyphaga, the non-culturable L. pneumophila were resuscitated and proliferated robustly within A. polyphaga. Upon treatment by NaOCl, uninfected amoebae differentiated into cysts within 48 h. In contrast, L. pneumophila-infected A. polyphaga failed to differentiate into cysts, and L. pneumophila was never detected in cysts of A. polyphaga. We conclude that amoebic trophozoites protect intracellular L. pneumophila from eradication by NaOCl, and play an essential role in resuscitation of VNC L. pneumophila in NaOCl-disinfected water sources. Intracellular L. pneumophila within trophozoites of A. polyphaga block encystation of the amoebae, and the resistance of both organisms to NaOCl is enhanced. To ensure long-term eradication and complete loss of the VNC state of L. pneumophila, we recommend that Legionella-protozoa co-culture should be an important tool to ensure complete loss of the VNC state of L. pneumophila.

  15. Coronectomy - A viable alternative to prevent inferior alveolar nerve injury

    Directory of Open Access Journals (Sweden)

    Alok Sagtani

    2015-12-01

    Full Text Available Background and Objectives: Coronectomy is a relatively new method to prevent the risk of Inferior Alveolar Nerve (IAN injury during removal of lower third molars with limited scientific literature among Nepalese patients. Thus, a study was designed to evaluate coronectomy regarding its use, outcomes and complications.Materials and Methods: A descriptive study was conducted from December 2012 to December 2013 among patients attending Department of Oral and Maxillofacial Surgery, College of Dental Sciences, BP Koirala Institute of Health Sciences, Dharan, Nepal for removal of mandibular third molars. After reviewing the radiograph for proximity of third molar to the IAN, coronectomy was advised. A written informed consent was obtained from the patients and coronectomy was performed. Patients were recalled after one week. The outcome measures in the follow-up visit were primary healing, pain, infection, dry socket, root exposure and IAN injury. The prevalence of IAN proximity of lower third molars and incidence of complications were calculated.Results: A total 300 mandibular third molars were extracted in 278 patients during the study period. Out of 300 impacted mandibular third molar, 41 (13.7% showed close proximity to inferior alveolar nerve . The incidence of complications and failed procedure was 7.4% among the patients who underwent coronectomy. During the follow up visit, persistent pain and root exposure was reported while other complications like inferior alveolar nerve injury, dry socket and infection was not experienced by the study patients.Conclusion: With a success rate of 92.6% among the 41 patients, coronectomy is a viable alternative to conventional total extraction for mandibular third molars who have a higher risk for damage to the inferior alveolar nerve.JCMS Nepal. 2015;11(3:1-5.

  16. Plan de mercadeo para una promotora de eventos corporativos

    OpenAIRE

    Burbano Zhindon, Andrea Cristina; Chavez Lopez, Maria Cecilia; Orlando Haro, Sofia Pierina; Miranda, Jorge Luis

    2009-01-01

    Este proyecto presenta una alternativa para todas aquellas empresas que realizan eventos frecuentemente, ofreciéndoles un servicio personalizado para la coordinación de cada uno de los detalles que conlleva la organización de un evento. El objetivo fundamental de este estudio es desarrollar un plan de mercadeo para el servicio antes mencionado, aplicando los principios del marketing para su correcta implementación.

  17. Agricultura familiar para el desarrollo rural incluyente

    Directory of Open Access Journals (Sweden)

    Laksmi Reddiar Krishnamurthy

    2017-04-01

    Full Text Available Las comunidades indígenas Mayas de la península de Yucatán han practicado la antigua tradición de agricultura familiar, en particular los huertos caseros, para garantizar su seguridad alimentaria. Con el objetivo de mejorar la práctica tradicional considerando paradigmas de la ciencia moderna, por una parte, se colectaron datos para definir la complejidad estructural y diversidad funcional a partir de 20 huertos familiares en cinco comunidades: X - Maben, X - Pichil, X - Yatil, San José II y Melchor Ocampo; y por otra, se organizaron grupos de discusión para dilucidar la estrategia de gestión practicada por las comunidades nativas. Los resultados mostraron que los huertos son manejados principalmente por las mujeres. También mostraron que el propósito principal del crecimiento y mantenimiento de los huertos familiares es garantizar la producción de alimentos nutritivos durante todo el año. Y, por último que los huertos caseros también sirven para propósitos secundarios tales como la provisión de productos y servicios para la medicina tradicional. El estudio sugiere que se debe de promover e invertir en huertos caseros para mejorar las estrategias de desarrollo incluyente en ambientes socio-culturales y biofísicos similares.

  18. Factores que influyen en el contenido de escualeno, fitoesteroles totales y esterificados en el subproducto ácidos grasos destilados de soya para su potencial aprovechamiento

    Directory of Open Access Journals (Sweden)

    José Rodríguez Rodríguez

    2015-01-01

    Full Text Available Una necesidad actual de la industria de extracción y refinación de aceite vegetal es la reducción y/o explotación de sus subproductos. Uno de estos, conocido como Ácidos Grasos Destilados de Aceite de Soya (AGDAS, resulta potencialmente valioso para las industrias alimentaria, farmacéutica y cosmética por la presencia de compuestos con actividad biológica favorable, como los fitoesteroles y el escualeno, los primeros como agentes hipocolesterolemiantes y el escualeno como emoliente y humectante. En este trabajo, mediante cromatografía de gases se determinó el contenido de escualeno y fitoesteroles libres y esterificados para evaluar la viabilidad de aprovechamiento de AGDAS como fuente de dichos compuestos y establecer si la procedencia del grano de soya (americano o nacional, la condición de cultivo (Sequía o Normal, años 2011 y 2012 respectivamente y el grado de calidad del aceite (Genérico o Premium son factores determinantes en el contenido de dichos compuestos en el subproducto. En conclusión AGDAS representa una fuente viable de fitoesteroles totales con 14.92% p/p, no así de escualeno con 1.75% p/p. La concentración de escualeno está influenciada por la procedencia del grano y la condición de cultivo, mientras que la de fitoesteroles esterificados por el grado de calidad y condición de cultivo. Ninguno de los factores influyó en el contenido de fitoesteroles totales. Esto permite establecer que la composición del subproducto AGDAS no es constante y se justifican protocolos como el de este estudio para su caracterización con fines de aprovechamiento.

  19. Patrones de diseño para mejorar la accesibilidad y uso de aplicaciones sociales para adultos mayores

    Directory of Open Access Journals (Sweden)

    Huizilopoztli Luna García

    2015-07-01

    Full Text Available El objetivo de este artículo fue proponer una colección no exhaustiva de 36 patrones para el diseño de interacciones en aplicaciones sociales para adultos mayores. La propuesta representa un punto de partida confiable para facilitar, a diseñadores/desarrolladores, la integración de usabilidad en las interfaces de estas tecnologías con el fin de fomentar su aceptación, uso y adopción entre los adultos mayores. Esta propuesta se basa en esfuerzos previos comúnmente expresados como criterios y guías de diseño para definir los posibles problemas de usabilidad en este tipo de interfaces, y se concentra en reforzar estos enfoques mediante la integración de una mayor descripción de tales anomalías y alternativas de solución bajo una estructura de patrones útil para los diseñadores/desarrolladores. Se realizó un estudio de usabilidad con la técnica «evaluación heurística» (comúnmente utilizada en interacción hombre-máquina para obtener la percepción de los usuarios sobre un diseño particular. El trabajo se llevó a cabo desde dos perspectivas: el punto de vista de expertos técnicos y la percepción de un grupo social de adultos mayores. Los resultados reflejaron que los patrones propuestos propician la creación de interfaces bien diseñadas capaces de ofrecer una mejor experiencia de uso y promueven un impacto positivo en la calidad de vida de los adultos mayores.

  20. Alimentos balanceados para perros en Costa Rica

    Directory of Open Access Journals (Sweden)

    Ruth Vargas

    2001-01-01

    Full Text Available Diagnóstico de la comercialización de alimentos balanceados para perros en Costa Rica. Para ampliar los reportes oficiales de la comercialización de alimentos para perros se creó una base de datos que incluyó cantidad, costo, empaque y formulación durante el período 1998 a agosto del 2000. De 1995 a 1998 y de 1996 a 1999 la producción nacional incrementó un 90,4% y la importación un 42,56% respectivamente, ocupando los alimentos nacionales un 72% del tonelaje y un 70% del valor en dólares americanos. No fue posible determinar cuál es el empaque más comercializado, pero si que los alimentos extrusados son los que ocupan el primer lugar y que los alimentos recomendados para cachorros y adultos son los que más se comercializan. La ausencia de datos en las declaraciones sugiere la necesidad de incrementar el control en las mismas en caso de requerirse un estudio de mercado, ya que contrariamente a esta situación el mayor número de garantías inscritas corresponden separadamente para las fases de cachorros y adultos. Merece también atención que las garantías indican mayores porcentajes de proteína cruda que los recomendados por la AAFCO y que al menos 36 fórmulas son recomendadas para estados sanitarios específicos (p.e. para perros con problemas de alergias, cálculos renales, pérdidas de pelo, etc. sin que oficialmente se encuentre registrada ninguna fórmula medicada

  1. Actitud Positiva: la base para la competitividad

    Directory of Open Access Journals (Sweden)

    Marco Anderson Espinoza

    2008-04-01

    Full Text Available Una estrategia empresarial enfocado en el mejoramiento continuo de la productividad para la competitividad, depende significativamente del manejo inteligente de tres variables visibles:Calidad, Entrega oportuna y Costo. No obstante, estas tres variables no suelen sersuficientes. La estrategia debe ser complementada con el manejo adecuado de variables intangibles como la Motivación y las Condiciones de Trabajo, con la aplicación de Tecnología Ambientalmente Amigable, en un marco de Actitud Positiva.A final de cuentas, la Actitud Positiva es fundamental para el recurso humano de cualquier organización, a fin de construir una plataforma sólida y estable que sirva de base para soportar un proceso sostenible de mejoramiento de la productividad, para aumentar la competitividad como estrategia empresarial exitosa.

  2. CONCEIVING AND GENERATING A VIABLE COMPLEXITY MODEL FOR PERSONAL AND/OR ENTREPRENEURIAL CHANGE AND DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    George Niculescu

    2012-03-01

    Full Text Available This article proposes on the one hand to do research and study – with a genuine approach, inspiredand deep-rooted in the method of the sociological school in Bucharest and creatively renewed and integrating thenewest acquisitions of complexity science – the representative intricate entity “man as an economic actor (resourceand potential within the industrial organization and corporation in mono-industrial area (region in the period inwhich the phenomenon of transition from an industrial society to knowledge society takes place; and on the otherhand our project proposes to conceive a viable model of change and career of the human resources in the ElectricPlant of Rovinari as well as to conceive and accomplish a pilot-model of a platform in order to support the efficientimplement through a research-action approach of the model of personal and/or entrepreneurship change and careerof the human resources in the Electric Plant of Rovinari.

  3. A Viable Microbial Community in a Subglacial Volcanic Crater Lake, Iceland

    Science.gov (United States)

    Gaidos, Eric; Lanoil, Brian; Thorsteinsson, Thorsteinn; Graham, Andrew; Skidmore, Mark; Han, Suk-Kyun; Rust, Terri; Popp, Brian

    2004-09-01

    We describe a viable microbial community in a subglacial lake within the Grímsvötn volcanic caldera, Iceland. We used a hot water drill to penetrate the 300-m ice shelf and retrieved lake water and volcanic tephra sediments. We also acquired samples of borehole water before and after penetration to the lake, overlying glacial ice and snow, and water from a nearby subaerial geothermal lake for comparative analyses. Lake water is at the freezing point and fresh (total dissolved solids = 260 mg L-1). Detectable numbers of cells were found in samples of the lake water column and tephra sediments: 2 × 104 ml-1 and 4 × 107 g-1, respectively. Plate counts document abundant cold-adapted cultivable organisms in the lake water, but not in the borehole (before penetration) or glacial ice. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments amplified from genomic DNA extracted from Gr??msv??tn samples indicates that the lake community is distinct from the assemblages of organisms in borehole water (before penetration) and the overlying ice and snow. Sequencing of selected DGGE bands revealed that many sequences are highly similar to known psychrophilic organisms or cloned DNA from other cold environments. Significant uptake of 14C-labeled bicarbonate occurred in dark, low-temperature incubations of lake water samples, indicating the presence of autotrophs. Acetylene reduction assays under similar incubation conditions showed no significant nitrogen fixation potential by lake water samples. This may be a consequence of the inhibition of diazotrophy by nitrogen in the lake.

  4. Avaliação da uniformidade de aplicação de água em um sistema de gotejamento para pequenas propriedades Uniformity of water application for a drip irrigation system to small farms

    Directory of Open Access Journals (Sweden)

    João Marcelo Silva do Nascimento

    2009-01-01

    Full Text Available O uso de sistemas de irrigação por gotejamento à baixa pressão tem como objetivo oferecer uma técnica de irrigação viável para pequenas propriedades. Foram dimensionados oito sistemas de irrigação, um com os seguintes acessórios: 210 metros de tubo gotejador com diâmetro interno (DI de 10,4 mm, sete conectores de 12 mm, e seis metros de tubo de polietileno, um adaptador para tubo de polietileno, uma curva de 90º e três metros de tubo de PVC com DI igual a 20,6 mm. Quatro sistemas foram montados com um filtro de tela de 130 μm e os demais com um filtro de disco de 200 μm. Em todos os sistemas foram utilizados um reservatório de 1 m³ localizado a 3 m acima do nível do solo. Irrigou-se 1296 m² de uma cultura de tomate, em canteiros com dimensões de 1,20 m por 60 m. Para avaliação técnica do projeto foi obtida a uniformidade de aplicação de água através do coeficiente de uniformidade de emissão (UE e coeficiente de uniformidade de distribuição (UD, durante um período de 90 dias. Os resultados indicaram que o UE e o UD foram excelentes e que os valores de grau de entupimento aumentaram com o uso da irrigação. Assim, de acordo com os resultados, comprova-se a viabilidade técnica da utilização de sistemas de irrigação por gotejamento funcionando a baixa pressão para pequenas áreas.A low pressure drip irrigation gravity feed system may be a viable technique to small farms. So, according with it, eight irrigation systems with 10.4 mm internal diameter driptubing 210 m long, seven 12 mm adapters, 20.6 mm polyethylene pipe 6 m long, polyethylene adapter, 20.6 mm 90º elbow, 20.6 mm PVC pipe 3 m long and 1 m³ water reservoir at 3 m height, were designed and assembled to irrigate 1.296 m² with tomato cultivated in 0.8 x 60 m beds. One 130 mm screen filter was used to four systems and one 200 mm disc filter for the other ones. Emission uniformity coefficients (UE and distribution uniformity coefficients (UD were

  5. Technological properties of kaolin from Para; Propriedades tecnologicas do caulin do Para

    Energy Technology Data Exchange (ETDEWEB)

    Hildebrando, E.; Martin-Cortes, G.R.; Esper, F.J.; Wiebeck, H.; Alves Junior, P.; Valenzuela-Diaz, F.R., E-mail: germac@usp.br [Universidade de Sao Paulo (PMT/EP/USP), Sao Paulo, SP (Brazil). Escola Politecnica. Departamento de Engenharia Metalurgica e de Materiais

    2011-07-01

    The NE of the State of Para, is notable for its large reserves of kaolin for paper. Kaolin is a mineral composed of hydrated aluminum silicates such as kaolinite and halloysite. The exploitation of these reserves to generate products with higher commercial value, especially in the industries of adsorbents and catalysts, is what motivates the continuous study of technological properties of the Amazon kaolin's. Thus, this paper presents a technological characterization of a sample of kaolin from Para State by X-ray diffraction, SEM - scanning electron microscopy and infrared spectroscopy. The results of preliminary tests indicate that the sample consists for the most part by kaolinite present in low concentrations of quartz and anatase. (author)

  6. Quantitative real-time PCR combined with propidium monoazide for the selective quantification of viable periodontal pathogens in an in vitro subgingival biofilm model.

    Science.gov (United States)

    Sánchez, M C; Marín, M J; Figuero, E; Llama-Palacios, A; León, R; Blanc, V; Herrera, D; Sanz, M

    2014-02-01

    Differentiation of live and dead cells is an important challenge when using molecular diagnosis for microbial identification. This is particularly relevant when bacteria have been exposed to antimicrobial agents. The objective of this study was to test a method using quantitative real-time polymerase chain reaction (qPCR) combined with propidium monoazide (PMA), developed for the selective quantification of viable P. gingivalis, A. actinomycetemcomitans, F. nucleatum and total bacteria in an in vitro biofilm model after antimicrobial treatment. PMA-qPCR method was tested in an in vitro biofilm model, using isopropyl alcohol as the antimicrobial agent. Matured biofilms were exposed for 1, 5, 10 and 30 min to isopropyl alcohol by immersion. Biofilms were disrupted and PMA added (final concentration of 100 μm). After DNA isolation, qPCR was carried out using specific primers and probes for the target bacteria. The differentiation of live and dead cells was tested by analysis of variance. When PMA was used in the presence of viable target bacterial cells, no statistically significant inhibition of qPCR amplification was detected (p > 0.05 in all cases). Conversely, after immersion in isopropyl alcohol of the biofilm, PMA resulted in a significant total reduction of qPCR amplification of about 4 log10 . P. gingivalis showed a vitality reduction in the biofilm of 3 log10 , while A. actinomycetemcomitans and F. nucleatum showed a 2 log10 reduction. These results demonstrate the efficiency of PMA for differentiating viable and dead P. gingivalis, A. actinomycetemcomitans and F. nucleatum cells, as well as total bacteria, in an in vitro biofilm model, after being exposed to an antimicrobial agent. Hence, this PMA-qPCR method may be useful for studying the effect of antimicrobial agents aimed at oral biofilms. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  7. Monitoring and Targeting Anti-VEGF Induced Hypoxia within the Viable Tumor by 19F–MRI and Multispectral Analysis

    Directory of Open Access Journals (Sweden)

    Yunzhou Shi

    2017-11-01

    Full Text Available The effect of anti-angiogenic agents on tumor oxygenation has been in question for a number of years, where both increases and decreases in tumor pO2 have been observed. This dichotomy in results may be explained by the role of vessel normalization in the response of tumors to anti-angiogenic therapy, where anti-angiogenic therapies may initially improve both the structure and the function of tumor vessels, but more sustained or potent anti-angiogenic treatments will produce an anti-vascular response, producing a more hypoxic environment. The first goal of this study was to employ multispectral (MS 19F–MRI to noninvasively quantify viable tumor pO2 and evaluate the ability of a high dose of an antibody to vascular endothelial growth factor (VEGF to produce a strong and prolonged anti-vascular response that results in significant tumor hypoxia. The second goal of this study was to target the anti-VEGF induced hypoxic tumor micro-environment with an agent, tirapazamine (TPZ, which has been designed to target hypoxic regions of tumors. These goals have been successfully met, where an antibody that blocks both murine and human VEGF-A (B20.4.1.1 was found by MS 19F–MRI to produce a strong anti-vascular response and reduce viable tumor pO2 in an HM-7 xenograft model. TPZ was then employed to target the anti-VEGF-induced hypoxic region. The combination of anti-VEGF and TPZ strongly suppressed HM-7 tumor growth and was superior to control and both monotherapies. This study provides evidence that clinical trials combining anti-vascular agents with hypoxia-activated prodrugs should be considered to improved efficacy in cancer patients.

  8. Desarrollo de manuales para la inspección y revisión de instalaciones petrolíferas para uso propio, para suministro a vehículos y transporte de mercancías peligrosas por carretera

    OpenAIRE

    Cardell Bilbao, Pablo Juan

    2010-01-01

    El objetivo de este proyecto es la realización de un manual que aúne y clarifique toda la documentación, la normativa y los procedimientos necesarios para cumplir las auditorías (revisiones e inspecciones) en las instalaciones existentes, los trámites para la apertura de nuevas instalaciones para suministro a vehículos (especialmente las Estaciones de Servicio (EESS), y para Usos Propios (UUPP) en Canarias, y el transporte de mercancías peligrosas por carretera (ADR), mostrando el procedimien...

  9. Oxitocina: nuevas perspectivas para una droga antigua

    OpenAIRE

    Steven L. CLARK; Kathleen Rice SIMPSON; G. Eric KNOX; Thomas J. GARITE

    2010-01-01

    La oxitocina es el medicamento más comúnmente asociada con la prevención de complicaciones perinatales y recientemente fue incluida por el Instituto para la Administración Medicamentosa Segura (Institute for Safe Medication Practices - ISMP) en una pequeña lista de medicamentos “con alto grado de riesgos”, lo que puede “necesitar salvaguardas especiales para reducir el riesgo de error”. Las recomendaciones actuales para administrar este medicamento son vagas en relación a la indicación, tiemp...

  10. INICIATIVAS PARA EL CAMBIO

    Directory of Open Access Journals (Sweden)

    César Alfonso Defrancisco-Larrañaga

    2013-07-01

    Full Text Available El planeta no atraviesa por su mejor momento en materia de conservación y renovación de sus recursos naturales. Claramente en los últimos tiempos los recursos hídricos han venido haciéndose cada vez menores; el hombre ha notado con preocupación y vivido en carne propia este fenómeno, que de no ser tratado a tiempo, podría atentar contra la existencia misma. Esto lo ha alentado a buscar métodos que sirvan como solución para contrarrestar éste mal y crear conciencia entre sus iguales, para así poder garantizar su permanencia y la de su entorno durante muchas generaciones. En países desarrollados del continente europeo y en algunos lugares de América del norte, el uso de sistemas de reutilización y tratamiento de aguas grises y aprovechamiento de las aguas lluvias se ha convertido en una constante y nos están dando las pautas para poder seguir el ejemplo y así de esta manera poder aportar a la causa ambiental y a su vez crear un ahorro en nuestros gastos mensuales de consumo.

  11. AUTONOMÍA PARA APRENDER Y AUTONOMÍA PARA VIVIR

    Directory of Open Access Journals (Sweden)

    Miryam Narváez Rivero

    2005-04-01

    Full Text Available RESUMEN: El sistema educativo tiene el reto actual de formar individuos autónomos. Esta autonomía no implica solo el que las personas tengan los recursos necesarios para aprender a aprender, sino que puedan convivir armónicamente en comunidad, escuchándose a sí mismos para tomar sus propias decisiones, sin estar determinados por deseos instintivos ni modelos coercitivos impuestos por la sociedad. Así, para desarrollar autonomía, le corresponde a la educación trabajar tres elementos: la voluntad, la libertad y la autoestima. De esta forma, una educación promotora de una verdadera autonomía deberá tener un carácter dialógico, haciendo hincapié en la integración solidaria de la persona con su comunidad. Nos referimos a una persona que no ha renunciado a sus metas ni aspiraciones, sino que sabe cómo guiarlas hacia un proyecto común de bienestar general. ABSTRACT: The educative system’s latest challenge is to educate independent individuals. This autonomy does not just imply that people have the necessary resources to learn to learn, but the ones they need to live together in harmony with others, listening to themselves to make their own decisions without being determined by instinctive desires nor coercive models imposed by the society. It is so, that to develop autonomy, the educational system must work with these three elements: will, freedom and self-esteem. Also, an educational system which promotes a true autonomy must have a dialogic character, insisting on the individual solidarity integration with the community; with this, we mean a person who has not resigned to his goals or aspirations, but that knows how to guide them towards a common project for the community well-being.

  12. Modelación y simulación de un pervaporador acoplado a un proceso de sacarificación-fermentación para la producción de etanol

    Directory of Open Access Journals (Sweden)

    Jairo Antonio Cubillos-Lobo

    2015-09-01

    Full Text Available Actualmente, la integración de procesos es considerada una opción viable para reducir costos en la producción de etanol a partir de biomasa. Simulaciones y resultados experimentales han demostrado los beneficios de la integración de las etapas de sacarificación y fermentación y del acoplamiento del proceso de fermentación a la recuperación in-situ de etanol por pervaporación en la producción de bioetanol; sin embargo, no se han publicado estudios de la integración del proceso de sacarificación-fermentación simultánea con membranas de separación, para la remoción insitu de etanol a partir del caldo de fermentación. En este trabajo se aborda esta necesidad, mediante la modelación y simulación de la producción de etanol a partir de almidón de yuca por sacarificación-fermentación simultánea, acopladas a un sistema de remoción in-situ de etanol por pervaporación, con membranas a base de polidimetilsiloxano (PDMS, silicalita y PDMS-Silicalita. La membrana de PDMS se modeló usando el mecanismo de solución-difusión, mientras que para la membrana de silicalita se utilizó el modelo de adsorción-difusión. Para el modelo del proceso de sacarificación-fermentación simultánea (SSF se utilizó una fusión del modelo multicadena (Michaelis–Menten, junto con un modelo tipo Monod. El ajuste del modelo de SSF a los datos experimentales reportados, así como de los modelos de membranas de PDMS y silicalita a los valores reportados en la literatura es bueno: la máxima desviación encontrada es del orden de 3%. El modelo integrado se utilizó para predecir la concentración de etanol en función del tiempo durante la sacarificación-fermentación simultánea.

  13. Significado de la calidad de vida del adulto mayor para sí mismo y para su familia

    Directory of Open Access Journals (Sweden)

    Martha Vera

    2007-09-01

    Full Text Available Objetivo: Comprender el significado que la expresión lingüística ‘calidad de vida del adulto mayor’ tiene para el adulto mayor y para su familia. Diseño: Estudio cualitativo, descriptivo, interpretativo - comprensivo, fundamentado en la Teoría de las Representaciones Sociales. Lugar: Asentamiento Humano Laura Caller, en Los Olivos. Participantes: Adultos mayores y sus respectivos grupos familiares. Métodos: Las técnicas e instrumentos utilizados fueron la observación, entrevista a profundidad y grupos focales; guía de observación y dos cuestionarios con ocho preguntas norteadoras, previamente validados. Principales medidas de resultados: Significado de calidad de vida para el adulto mayor y para la familia. Resultados: Participaron dieciséis adultos mayores con sus respectivos grupos familiares, entre 2004 y 2006. Los discursos a través de la ‘objetivación’ y el ‘anclaje’ y los análisis general, ideográfico-axial y nomotético-selectivo permitieron la emergencia de cuatro representaciones sociales: a conocimiento sobre la vejez y ser un adulto mayor; b elementos principales y secundarios del significado de calidad de vida del adulto mayor; c valores y actitudes del adulto mayor y familia sobre la calidad de vida; y, d necesidades del adulto mayor como ser social y la calidad de vida. Conclusiones: Para el adulto mayor, calidad de vida significa tener paz y tranquilidad, ser cuidado y protegido por la familia con dignidad, amor y respeto, y tener satisfechas como ser social sus necesidades de libre expresión, decisión, comunicación e información. Para la familia, que el adulto mayor satisfaga sus necesidades de alimentación, vestido, higiene y vivienda.

  14. Nuclear reactor PBMR and cogeneration; Reactor nuclear PBMR y cogeneracion

    Energy Technology Data Exchange (ETDEWEB)

    Ramirez S, J. R.; Alonso V, G., E-mail: ramon.ramirez@inin.gob.mx [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico)

    2013-10-15

    In recent years the nuclear reactor designs for the electricity generation have increased their costs, so that at the moment costs are managed of around the 5000 US D for installed kw, reason for which a big nuclear plant requires of investments of the order of billions of dollars, the designed reactors as modular of low power seek to lighten the initial investment of a big reactor dividing the power in parts and dividing in modules the components to lower the production costs, this way it can begin to build a module and finished this to build other, differing the long term investment, getting less risk therefore in the investment. On the other hand the reactors of low power can be very useful in regions where is difficult to have access to the electric net being able to take advantage of the thermal energy of the reactor to feed other processes like the water desalination or the vapor generation for the processes industry like the petrochemical, or even more the possible hydrogen production to be used as fuel. In this work the possibility to generate vapor of high quality for the petrochemical industry is described using a spheres bed reactor of high temperature. (Author)

  15. ÉTICA PARA O DESENVOLVIMENTO SUSTENTÁVEL – UMA RESPONSABILIDADE PARA COM AS GERAÇÕES FUTURAS

    Directory of Open Access Journals (Sweden)

    Patrícia Melo Silva

    2011-02-01

    Full Text Available O trabalho apresenta uma discussão sobre como a ética pode contribuir para a ciência se desenvolver de maneira sustentável com o intuito de garantir a biodiversidade em uma sociedade que cresce de acordo com a moral utilitarista. Os principais aspectos analisados foram questões da ética como proposta oposta à moral utilitarista, além de aspectos relacionados à economia global, ao sistema capitalista e à interdisciplinaridade. O principal objetivo do trabalho é analisar como a ética pode contribuir para o desenvolvimento sustentável e para o respeito à biodiversidade. A metodologia utilizada foi a de análise bibliográfica.

  16. Nueva Sede para la Academia

    Directory of Open Access Journals (Sweden)

    Mario Camacho Pinto

    1994-06-01

    Full Text Available

    Una nueva sede adecuadamente dotada siempre estuvo en la mente de nuestros expresidentes Académicos César Augusto Pantoja, Hernando Groot, Pablo Gómez Martínez, Jorge Cavelier Gaviria y Efraim Otero-Ruiz, con quienes me tocó en suerte trabajar 12 años consecutivos, cerca de ellos a través de mi cargo de Coordinardor de la Comisión Permanente de Biblioteca y Publicaciones y de la Revista MEDICINA que se ha venido publicando sin interrupciones dentro de un compañerismo ejemplar; además de otras distinciones operativas que me inducen a expresar gratitud.

    Ahora, además, como Secretario de la Coporación por elección unánime de la Asamblea hace dos años, he sido testigo ático de una intensa, inteligente e inagotable actividad que nuestro Presidente Académico Juan Jacobo Muñoz Delgado quien siempre con el visto bueno de la Junta Directiva, mediante un acelerado ritmo de trabajo se impuso la tarea de convertir en realidad inmediata tan anhelada y necesaria idea.

    En efecto, la nueva sede fue inaugurada oficialmente el día 2 de septiembre de 1993.

    Este Editorial es la constancia escrita de tal febril actividad que para proporcionar evidencia a los lectores de “MEDICINA” resumo en breves incisos que muestran el tránsito a un estado con superávit de excepción para esta clase de instituciones:

    1. Terminación de la obra ya iniciada de la remodelación arquitectónica, tanto exterior de seguridad y aspecto como interior con suficientes y modernas instalaciones.

    2. Cerramiento total con altas y elegantes rejas metálicas y doble portería por la calle 69 y la carrera 7a.

    3. Aparcadero lujosamente adoquinado, amplio y seguro para 30 automóviles.

    4. Decoración interior estéticamente consultada, bien lograda en cortinas y clásicas lámparas de cristal obsequiadas por conocidos académicos. Un par de originales urnas precolombianas en el hall de entrada.

    5. Zona social doble, amplia

  17. Hacia un nuevo diseño para el aprendizaje: escenarios educativos para la Web 2.0

    Directory of Open Access Journals (Sweden)

    Alejandro Canales Cruz

    2014-10-01

    Full Text Available En este artículo se retoma la caracterización de pedagogía en la virtualidad, la cual se fundamenta en la apropiación tecnológica en función de la educación y voluntad de los participantes. Se propone la convergencia del diseño tecnopedagógico, unido a la apropiación creativa y a los usos pedagógicos efectivos para maximizar el proceso educativo. Se plantea el empleo de la especificación IMS Learning Design (LD para la construcción de escenarios educativos como un proceso sistémico con actividades interrelacionadas que permiten crear ambientes que en verdad faciliten, de forma mediada, el proceso educativo. Finalmente, se presenta una herramienta para la autoría de escenarios educativos.

  18. Modelo de negocio para bebidas funcionales Ceiba

    OpenAIRE

    Gil Giraldo, Jhonatan; Restrepo Mesa, Juan Eduardo

    2017-01-01

    El presente documento, modelo de negocio para bebidas naturales Ceiba, es el trabajo de grado realizado por los autores con el fin de optar al título de magíster en Administración (MBA) de la Universidad EAFIT -- El cual esta soportado en los diferentes conceptos y metodologías de modelo de negocio y planes de negocio -- Por tratarse de un prototipo que busca definir los elementos estratégicos necesarios para su desarrollo, arrojó como resultado que la metodología más adecuada para ser aplica...

  19. Middleware Cerberus usando RFID para rastreabilidade bovina

    OpenAIRE

    Silva, Márcio Roberto

    2009-01-01

    A gerência na zootecnia de precisão é cada vez mais necessária para garantia de lucros e a conquista de novos mercados os quais exigem qualidade e certificação. Nesta dissertação é abordado um sistema de rastreabilidade para o controle na bovinocultura utilizando chips RFID. Sendo que a identificação segura dos animais é a base para a rastreabilidade bovina e bubalina. Em vista as exigências do mercado consumidor o governo brasileiro instituiu o Sistema Brasileiro de Identificação e Certifica...

  20. Test para la profilaxis de la prematuridad

    OpenAIRE

    Cruz Laguna, Gladys María

    2008-01-01

    La prematuridad constituye un problema mundial de salud, la comunidad científica busca predictores para disminuir su incidencia. La investigación persiguió obtener una escala de puntuación que modele las características cervicales y permita diagnosticar la condición de riesgo de las gestantes para extender el período de gestación y lograr nacimientos al término. Se realizó un cuasiexperimento en gestantes con factores de riesgo para la prematuridad del municipio Holguín. La muestra fue confor...