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Sample records for coffee coffea arabica

  1. DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica

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    Meynarti Sari Dewi Ibrahim

    2013-10-01

    Full Text Available Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1. The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.

  2. Antioxidant effect of Arabian coffee ( Coffea arabica L) blended with ...

    African Journals Online (AJOL)

    Purpose: To investigate the antioxidant activity of Coffea Arabica L in high-fat diet (HFD)-fed C57BL/6J mice. Methods: A decoction of Arabian coffee blended with or without cardamom or cloves was orally administered to HFD-fed C57BL/6J mice for a period of 60 days. At the end of the treatment, blood and tissue samples ...

  3. Microbial inputs in coffee (Coffea arabica L.) production systems, southwestern Ethiopia

    OpenAIRE

    Muleta, Diriba

    2007-01-01

    Arabica coffee is the key cash crop and top mainstay of the Ethiopian economy and requires sustainable production methods. Southwestern natural forests, the site of this study, are believed to be the centre of origin and diversity for Coffea arabica and still harbour wild Arabica coffee that may serve as an important gene pool for future breeding. Cost reductions, sustainability and quality improvement are now the major priorities in coffee production systems and require organic growing of co...

  4. The economic value of coffee (Coffea arabica) genetic resources

    NARCIS (Netherlands)

    Hein, L.G.; Gatzweiler, F.

    2006-01-01

    Whereas the economic value of genetic diversity is widely recognized there are, to date, relatively few experiences with the actual valuation of genetic resources. This paper presents an analysis of the economic value of Coffea arabica genetic resources contained in Ethiopian highland forests. The

  5. Genetic Diversity of Arabica Coffee (Coffea arabica L. in Nicaragua as Estimated by Simple Sequence Repeat Markers

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    Mulatu Geleta

    2012-01-01

    Full Text Available Coffea arabica L. (arabica coffee, the only tetraploid species in the genus Coffea, represents the majority of the world’s coffee production and has a significant contribution to Nicaragua’s economy. The present paper was conducted to determine the genetic diversity of arabica coffee in Nicaragua for its conservation and breeding values. Twenty-six populations that represent eight varieties in Nicaragua were investigated using simple sequence repeat (SSR markers. A total of 24 alleles were obtained from the 12 loci investigated across 260 individual plants. The total Nei’s gene diversity (HT and the within-population gene diversity (HS were 0.35 and 0.29, respectively, which is comparable with that previously reported from other countries and regions. Among the varieties, the highest diversity was recorded in the variety Catimor. Analysis of variance (AMOVA revealed that about 87% of the total genetic variation was found within populations and the remaining 13% differentiate the populations (FST=0.13; P<0.001. The variation among the varieties was also significant. The genetic variation in Nicaraguan coffee is significant enough to be used in the breeding programs, and most of this variation can be conserved through ex situ conservation of a low number of populations from each variety.

  6. Considerations in breeding for improved yield and quality in arabica coffee (Coffea arabica L.)

    NARCIS (Netherlands)

    Walyaro, D.J.A.

    1983-01-01

    Variation for growth., yield and quality characters was studied in a diallel cross among 11 varieties of arabica coffee. The objective was to demonstrate the possible application of such information in breeding programmes for improved yield and coffee quality.
    Growth characters

  7. Productivity of coffee crop (Coffea arabica L.) in conversion to the organic production system

    OpenAIRE

    Malta, Marcelo Ribeiro; Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG; Pereira, Rosemary Gualberto Fonseca Alvarenga; Universidade Federal de Lavras - UFLA; Chagas, Sílvio Júlio de Rezende; Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG; Guimarães, Rubens José; Universidade Federal de Lavras - UFLA

    2008-01-01

    This experiment was carried out in Lavras, MG, to verify the productivity of coffee crop (Coffea arabica L.) in conversion to the organic production system. The experiment was set in a six-year old coffee crop of the cultivar Catuaí Amarelo IAC 86, with spacing of 4,0 x 0,6 m, previously cultivated under the conventional system. In the organic treatments a 4 x 4 balanced lattice design with 5 replications in a 3 x 2 x 2 factorial scheme was used, besides 4 additional treatments. The f...

  8. Exogenous gibberellins inhibit coffee (Coffea arabica cv. Rubi) seed germination and cause cell death in the embryo

    NARCIS (Netherlands)

    Silva, Da E.A.A.; Toorop, P.E.; Nijsse, J.; Bewley, J.D.; Hilhorst, H.W.M.

    2005-01-01

    The mechanism of inhibition of coffee (Coffea arabica cv. Rubi) seed germination by exogenous gibberellins (GAs) and the requirement of germination for endogenous GA were studied. Exogenous GA4+7 inhibited coffee seed germination. The response to GA4+7 showed two sensitivity thresholds: a lower one

  9. Antioxidant effect of Arabian coffee (Coffea arabica L) blended with ...

    African Journals Online (AJOL)

    antioxidants (GSH, vitamins C and E) were significantly elevated (p < 0.05) in mice administered. Arabian coffee ... cancer [9,10]. In addition ... HFD alone. IV. HFD + Arabian coffee + cardamom. V. HFD + Arabian coffee + cardamom + cloves.

  10. Sensorial analysis of irradiated coffee (Coffea arabica L.) by electron beam

    International Nuclear Information System (INIS)

    Rodrigues, Flavio T.; Fanaro, Gustavo B.; Koike, Amanda C.R.; Villavicencio, Anna Lucia C.H.; Silva, Maria E.M. Pinto e

    2013-01-01

    Coffee is an important commodity and it is one of the most widely consumed beverages in the world. The acceptance of coffee by consumers depends mainly on the sensory characteristics of the beverage, that is its flavor, body, color, acidity and aroma. Food irradiation is processing technology environmental friendly and safety which aimed at the improvement of food quality. Depending on the absorbed radiation dose various effects can be achieved resulting in increase the shelf life, disinfestation, microorganism load reduction, without causing sensory changes to the food. Sensory analysis is the examination of a food through the evaluation of the attributes sensorial of product. The objective this paper was to evaluate the sensory properties, acceptance and purchase intent by the consumer of coffee (Coffea arabica L.) after the irradiation process with doses 6.0, 12.0 and 18.0kGy by electron beam. (author)

  11. Sensorial analysis of irradiated coffee (Coffea arabica L.) by electron beam

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Flavio T.; Fanaro, Gustavo B.; Koike, Amanda C.R.; Villavicencio, Anna Lucia C.H., E-mail: flaviot@ymail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Silva, Maria E.M. Pinto e [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Fac. de Saude Publica. Dept. de Nutricao

    2013-07-01

    Coffee is an important commodity and it is one of the most widely consumed beverages in the world. The acceptance of coffee by consumers depends mainly on the sensory characteristics of the beverage, that is its flavor, body, color, acidity and aroma. Food irradiation is processing technology environmental friendly and safety which aimed at the improvement of food quality. Depending on the absorbed radiation dose various effects can be achieved resulting in increase the shelf life, disinfestation, microorganism load reduction, without causing sensory changes to the food. Sensory analysis is the examination of a food through the evaluation of the attributes sensorial of product. The objective this paper was to evaluate the sensory properties, acceptance and purchase intent by the consumer of coffee (Coffea arabica L.) after the irradiation process with doses 6.0, 12.0 and 18.0kGy by electron beam. (author)

  12. Use of a draft genome of coffee (Coffea arabica) to identify SNPs associated with caffeine content.

    Science.gov (United States)

    Tran, Hue T M; Ramaraj, Thiruvarangan; Furtado, Agnelo; Lee, Leonard Slade; Henry, Robert J

    2018-03-07

    Arabica coffee (Coffea arabica) has a small gene pool limiting genetic improvement. Selection for caffeine content within this gene pool would be assisted by identification of the genes controlling this important trait. Sequencing of DNA bulks from 18 genotypes with extreme high- or low-caffeine content from a population of 232 genotypes was used to identify linked polymorphisms. To obtain a reference genome, a whole genome assembly of arabica coffee (variety K7) was achieved by sequencing using short read (Illumina) and long-read (PacBio) technology. Assembly was performed using a range of assembly tools resulting in 76 409 scaffolds with a scaffold N50 of 54 544 bp and a total scaffold length of 1448 Mb. Validation of the genome assembly using different tools showed high completeness of the genome. More than 99% of transcriptome sequences mapped to the C. arabica draft genome, and 89% of BUSCOs were present. The assembled genome annotated using AUGUSTUS yielded 99 829 gene models. Using the draft arabica genome as reference in mapping and variant calling allowed the detection of 1444 nonsynonymous single nucleotide polymorphisms (SNPs) associated with caffeine content. Based on Kyoto Encyclopaedia of Genes and Genomes pathway-based analysis, 65 caffeine-associated SNPs were discovered, among which 11 SNPs were associated with genes encoding enzymes involved in the conversion of substrates, which participate in the caffeine biosynthesis pathways. This analysis demonstrated the complex genetic control of this key trait in coffee. © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  13. Mechanical behaviour of Arabica coffee (Coffea arabica) beans under loading compression

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    Sigalingging, R.; Herak, D.; Kabutey, A.; Sigalingging, C.

    2018-02-01

    The uniformity of the product of the grinding process depends on various factors including the brittleness of the roasted coffee bean and it affects the extraction of soluble solids to obtain the coffee brew. Therefore, the reaching of a certain degree of brittleness is very important for the grinding to which coffee beans have to be subjected to before brewing. The aims of this study to show the mechanical behaviour of Arabica coffee beans from Tobasa (Indonesia) with roasted using different roasting time (40, 60 and 80 minutes at temperature 174 °C) under loading compression 225 kN. Universal compression testing machine was used with pressing vessel diameter 60 mm and compression speed 10 mm min-1 with different initial pressing height ranging from 20 to 60 mm. The results showed that significant correlation between roasting time and the brittleness.

  14. Influence of the fungi population on the physicochemical and chemical composition of coffee (Coffea arabica L.

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    Liliana Auxiliadora Avelar Pereira Pasin

    2011-09-01

    Full Text Available The influence of fungi associated with coffee fruits was verified regarding the chemical and physicochemical composition of Coffea arabica L. raw grains. The fruits were harvested at EPAMIG Experimental farm in Lavras, State of Minas Gerais - making up the different samples here analyzed. After processing and drying, the grains were incubated in wet chamber for fungal exteriorization through the blotter test method and submitted to the following analyses: polyphenoloxidase, total reducing and non-reducing sugars, clorogenic acid, titrable acidity, potassium leaching, electric conductivity and caffeine. The occurrence of the P. variable, P. rugulosum, P. funiculosum, F. equiseti, F. semitectum, A.alutaceus, A. niger and C. cladosporioides fungi in the different samples was detected. From the analysis of the results obtained, it was observed that the presence of the Aspergillus alutaceus fungus reduces the activity of the enzyme polyphenoloxidase and increases the values of potassium leaching, electric conductivity and chlorogenic acid. The incidence of the Cladosporium cladosporioides fungus influenced the average values of potassium leaching and electric conductivity.

  15. Estimation of leaf area in coffee leaves (Coffea arabica L. of the Castillo® variety

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    Carlos Andrés Unigarro-Muñoz

    2015-01-01

    Full Text Available Allometric models based on measurements of single leaf dimensions or a combination there are useful tools for determining individual leaf area (LA because they are non-destructive, precise, simple and economical methods. The present study was carried out at the Central Station Naranjal of Cenicafé, located in the Department of Caldas (Colombia, four models were defined using the variables length (L and/or width (W to estimate LA in coffee leaves of the Castillo® variety (Coffea arabica L.. Estimation of regression coefficients was performed using information recorded from 6,441 leaves (group 1, and their validation was performed using records from another 992 leaves (group 2. Leaves were collected from all strata of the canopy and ranged from 0.76 to 140 cm2 in LA. In addition to exhibiting coefficients of variation differing from zero based on t-tests at 1%, the evaluated models possess coefficients of determination between 0.93 and 0.99. Four expressions have developed and adjusted to estimate leaf area in individual leaves, based on the measurement of simple variables and non-destructive.

  16. Use of coffee (Coffea arabica pulp for the production of briquettes and pellets for heat generation

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    Robert Cubero-Abarca

    2014-10-01

    Full Text Available Coffee bean (Coffea arabica processing generates high amount of residues that are sources of environmental pollution. Therefore, an appropriate solution is needed. The objective of this study was to determine the potential of coffee pulp to produce briquettes and pellets. The study included pulp drying (using air, solar and hot air methods; the production of briquettes and pellets; the evaluation of their energy, physical and mechanical properties; and the evaluation of pellet quality using X-ray densitometry. The results showed that the pulp presented an initial moisture content of 90%, resulting in drying times of 699, 308 and 55 hours for air, solar and hot air drying, respectively, and the calorific values of the pellets and briquettes were 12,501 kJ kg-1 and 11,591 kJ kg-1, respectively. The ash content was 8.68% for the briquettes and 6.74% for the pellets. The density of the briquettes was 1,110 kg m-3, compared with 1,300 kg m-3 for the pellets. The apparent densities were 1,000 kg m-3 and 600 kg m-3 for the briquettes and pellets, respectively, and the water absorptions by the briquettes were 7.90% and 8.10% by the pellets. The maximum horizontal compression effort was 26.86 kg cm-2, measured in the pellets, compared with 4.52 kg cm-2 in the briquettes. The maximum horizontal load was 93.24 kg, measured in the briquettes, compared with 33.50 kg in the pellets. The value of the pellet durability test was 75.54%. X-ray densitometry showed that the pellet was uniform and a few cracks were observed on the pellet surface.

  17. Effect of gamma radiation (60Co) on the growth of Aspergillus ochraceus coffee (Coffea arabica L.)

    International Nuclear Information System (INIS)

    Rodrigues, Flavio T.; Duarte, Renato C.; Fanaro, Gustavo B.; Santillo, Amanda G.; Villavicencio, Anna Lucia C.H.

    2011-01-01

    Coffee is a major product on the world market. Its acceptance depends strongly on the sensory characteristics of the beverage, that is its aroma and flavor. One of the most important aspects of coffee culture today is the cup quality as such all segments of coffee production network are concentrating efforts to improve this trait. Foods that have been contaminated with fungi may contain may produce many mycotoxins including ochratoxim A (OTA). To prevent OTA in foodstuffs, it is necessary detect the producing fungi early. Ionizing radiation is a safe, environmentally clean, energy efficient process that can be used to increase the quality and reducing the microbiological contamination of the coffee. The aim of the present study was to evaluate the effects of different gamma radiation doses (0.0, 6.0, 12.0 and 18.0 kGy) on the growth of Aspergillus ochraceus in coffee (Coffee arabica L.). The analysis were performed to determine the fungi contamination the results were expressed as the viable counts per gram of sample (CFU/g) coffee samples irradiated and unirradiated. The results shows that microbiological contamination of coffee disagrees when increase doses of irradiation. The radiation doses 6.0, 12.0 and 18.0 kGy used resulted in a elimination of the number of Aspergillus ochraceus CFU/g when compared to the nonirradiated control group. Under the present conditions, gamma radiation was found to be an alternative for the control of Aspergillus ochraceus. (author)

  18. Ochratoxigenic fungi associated with green coffee beans (Coffea arabica L.) in conventional and organic cultivation in Brazil.

    Science.gov (United States)

    de Fátima Rezende, Elisângela; Borges, Josiane Gonçalves; Cirillo, Marcelo Ângelo; Prado, Guilherme; Paiva, Leandro Carlos; Batista, Luís Roberto

    2013-01-01

    The genera Aspergillus comprises species that produce mycotoxins such as aflatoxins, ochratoxins and patulin. These are cosmopolitan species, natural contaminants of agricultural products. In coffee grains, the most important Aspergillus species in terms of the risk of presenting mycotoxins belong to the genera Aspergillus Section Circumdati and Section Nigri. The purpose of this study was to assess the occurrence of isolated ochratoxigenic fungi of coffee grains from organic and conventional cultivation from the South of Minas Gerais, Brazil, as well as to evaluate which farming system presents higher contamination risk by ochratoxin A (OTA) produced by fungi. Thirty samples of coffee grains (Coffea arabica L.) were analysed, being 20 of them of conventional coffee grains and 10 of them organic. The microbiological analysis was done with the Direct Plating Technique in a Dichloran Rose Bengal Chloramphenicol Agar (DRBC) media. The identification was done based on the macro and micro morphological characteristics and on the toxigenic potential with the Plug Agar technique. From the 30 samples analysed, 480 filamentous fungi of the genera Aspergillus of the Circumdati and Nigri Sections were isolated. The ochratoxigenic species identified were: Aspergillus auricoumus, A. ochraceus, A. ostianus, A. niger and A. niger Aggregate. The most frequent species which produces ochratoxin A among the isolated ones was A. ochraceus, corresponding to 89.55%. There was no significant difference regarding the presence of ochratoxigenic A. ochreceus between the conventional and organic cultivation systems, which suggests that the contamination risk is similar for both cultivation systems.

  19. CARACTHERIZATION OF BIOMASS ENERGY AND CARBONIZATION OF COFFEE GRAINS (Coffea arabica, L AND (Cedrelinga catenaeformis, DUKE WOOD RESIDUES

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    Ailton Teixeira do Vale

    2007-12-01

    Full Text Available Brazil produces annually two million tons of coffee s husks from farms or industrial processing units. This wastematerial can be used for energy production; currently it is mainly used in agricultural practices as field straw cover up. This paperdeals with coffee s (Coffea arabica, L husks biomass energy characteristics, including wood carbonization. As a reference, the samestudy was performed with a wood species regularly used for building construction named Cedrorana (Cedrelinga catenaeformis,Duke. Coffee s husks was obtained from a farm 150 km far from Brasilia city and cedrorana sawdust from a local saw mill. Thispaper presents results from energy and biomass variables like moisture content, bulk density, lower and superior heating power, ashcontent, fixed carbon, volatile matter and volumetric energy. It has also studied carbonization, charcoal, pyroligneous licqor and noncondensablegases. A comparison between Coffee s husk with 0% moisture content and Cedrorana sawdust portrays the followingresults: bulk density 144.41 kg/m3, fixed carbon 10.31%, superior heating power 4.57 kWh (or 16.46 MJ or 3.933 Mcal/kg, charcoalcontent 40,64% and heating value per cubic meter 2,179 MJ/m3

  20. Arbuscular mycorrhizal fungi associated with shade trees and Coffea arabica L. in a coffee-based agroforestry system in Bonga, Southwestern Ethiopia

    OpenAIRE

    Sewnet ,Tadesse Chanie; Tuju, Fassil Assefa

    2013-01-01

    In a first step to understand the interactions between Coffea arabica L. trees and mycorrhizae in Ethio¬pia, an investigation of the current mycorrhizal colonization status of roots was undertaken. We sampled 14 shade tree species occurring in coffee populations in Bonga forest, Ethiopia. Milletia fer¬ruginea, Schefflera abyssinica, Croton macrostachyus, Ficus vasta, F. sur, Albizia gummifera, Olea capensis, Cordia africana, Ehretia abyssinica, Pouteria adolfi-friederici, Pavetta oliveriana, ...

  1. α-Amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits α-amylases from the coffee berry borer pest

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    Oliveira-Neto Osmundo B

    2010-06-01

    Full Text Available Abstract Background Coffee is an important crop and is crucial to the economy of many developing countries, generating around US$70 billion per year. There are 115 species in the Coffea genus, but only two, C. arabica and C. canephora, are commercially cultivated. Coffee plants are attacked by many pathogens and insect-pests, which affect not only the production of coffee but also its grain quality, reducing the commercial value of the product. The main insect-pest, the coffee berry borer (Hypotheneumus hampei, is responsible for worldwide annual losses of around US$500 million. The coffee berry borer exclusively damages the coffee berries, and it is mainly controlled by organochlorine insecticides that are both toxic and carcinogenic. Unfortunately, natural resistance in the genus Coffea to H. hampei has not been documented. To overcome these problems, biotechnological strategies can be used to introduce an α-amylase inhibitor gene (α-AI1, which confers resistance against the coffee berry borer insect-pest, into C. arabica plants. Results We transformed C. arabica with the α-amylase inhibitor-1 gene (α-AI1 from the common bean, Phaseolus vulgaris, under control of the seed-specific phytohemagglutinin promoter (PHA-L. The presence of the α-AI1 gene in six regenerated transgenic T1 coffee plants was identified by PCR and Southern blotting. Immunoblotting and ELISA experiments using antibodies against α-AI1 inhibitor showed a maximum α-AI1 concentration of 0.29% in crude seed extracts. Inhibitory in vitro assays of the α-AI1 protein against H. hampei α-amylases in transgenic seed extracts showed up to 88% inhibition of enzyme activity. Conclusions This is the first report showing the production of transgenic coffee plants with the biotechnological potential to control the coffee berry borer, the most important insect-pest of crop coffee.

  2. Alpha-amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits alpha-amylases from the coffee berry borer pest.

    Science.gov (United States)

    Barbosa, Aulus E A D; Albuquerque, Erika V S; Silva, Maria C M; Souza, Djair S L; Oliveira-Neto, Osmundo B; Valencia, Arnubio; Rocha, Thales L; Grossi-de-Sa, Maria F

    2010-06-17

    Coffee is an important crop and is crucial to the economy of many developing countries, generating around US$70 billion per year. There are 115 species in the Coffea genus, but only two, C. arabica and C. canephora, are commercially cultivated. Coffee plants are attacked by many pathogens and insect-pests, which affect not only the production of coffee but also its grain quality, reducing the commercial value of the product. The main insect-pest, the coffee berry borer (Hypotheneumus hampei), is responsible for worldwide annual losses of around US$500 million. The coffee berry borer exclusively damages the coffee berries, and it is mainly controlled by organochlorine insecticides that are both toxic and carcinogenic. Unfortunately, natural resistance in the genus Coffea to H. hampei has not been documented. To overcome these problems, biotechnological strategies can be used to introduce an alpha-amylase inhibitor gene (alpha-AI1), which confers resistance against the coffee berry borer insect-pest, into C. arabica plants. We transformed C. arabica with the alpha-amylase inhibitor-1 gene (alpha-AI1) from the common bean, Phaseolus vulgaris, under control of the seed-specific phytohemagglutinin promoter (PHA-L). The presence of the alpha-AI1 gene in six regenerated transgenic T1 coffee plants was identified by PCR and Southern blotting. Immunoblotting and ELISA experiments using antibodies against alpha-AI1 inhibitor showed a maximum alpha-AI1 concentration of 0.29% in crude seed extracts. Inhibitory in vitro assays of the alpha-AI1 protein against H. hampei alpha-amylases in transgenic seed extracts showed up to 88% inhibition of enzyme activity. This is the first report showing the production of transgenic coffee plants with the biotechnological potential to control the coffee berry borer, the most important insect-pest of crop coffee.

  3. Inoculation of starter cultures in a semi-dry coffee (Coffea arabica) fermentation process.

    Science.gov (United States)

    Evangelista, Suzana Reis; Miguel, Maria Gabriela da Cruz Pedrozo; Cordeiro, Cecília de Souza; Silva, Cristina Ferreira; Pinheiro, Ana Carla Marques; Schwan, Rosane Freitas

    2014-12-01

    The aim of this study was to evaluate the use of yeasts as starter cultures in coffee semi-dry processing. Arabica coffee was inoculated with one of the following starter cultures: Saccharomyces cerevisiae UFLA YCN727, S. cerevisiae UFLA YCN724, Candida parapsilosis UFLA YCN448 and Pichia guilliermondii UFLA YCN731. The control was not inoculated with a starter culture. Denaturing gradient gel electrophoresis (DGGE) was used to assess the microbial population, and organic acids and volatile compounds were quantified by HPLC and HS-SPME/GC, respectively. Sensory analyses were evaluated using the Temporal Dominance of Sensations (TDS). DGGE analysis showed that the inoculated yeasts were present throughout the fermentation. Other yeast species were also detected, including Debaryomyces hansenii, Cystofilobasidium ferigula and Trichosporon cavernicola. The bacterial population was diverse and was composed of the following genera: Weissella, Leuconostoc, Gluconobacter, Pseudomonas, Pantoea, Erwinia and Klebsiella. Butyric and propionic acids, were not detected in any treatment A total of 47 different volatiles compounds have been identified. The coffee inoculated with yeast had a caramel flavor that was not detected in the control, as assessed by TDS. The use of starter cultures during coffee fermentation is an interesting alternative for obtaining a beverage quality with distinctive flavor. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Physiological Dose-Response of Coffee (Coffea arabica L. Plants to Glyphosate Depends on Growth Stage Respuesta Fisiológica de Plantas de Café (Coffea arabica L. a Glifosato Depende de la Etapa de Crecimiento

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    Leonardo Bianco de Carvalho

    2012-06-01

    Full Text Available Glyphosate is the main herbicide used in coffee (Coffea arabica L. plantations in Brazil. Problems with herbicide drift commonly occur in orchard fields due to non-adequate spraying conditions. A series of experiments was carried out aiming to evaluate physiological dose-response of C. arabica plants submitted to exposure to simulated glyphosate drift in two distinct plant growth stages. Glyphosate was applied at 0, 180, 360, and 720 g acid equivalent (AE ha-1 directly on coffee plants with 10 and 45 d after transplanting (DAT. Glyphosate doses in a range of 180-360 g AE ha-1 increased photosynthesis, transpiration and stomatal conductance in 10 DAT-plants up to 14 d after application (DAA while, in 45 DAT-plants, an increase was observed only up to 2 DAA, but this pattern was not persistent afterwards so that no difference in gas exchange was observed at 60 DAA in both plants. Macronutrient content was not affected by glyphosate application in both plants. Plant DM accumulation was not affected by glyphosate application at 10 DAT-plants, but an increase in plant growth was observed when glyphosate was applied in a range of 360-720 g AE ha-1 in 45 DAT-plants. Coffea arabica cv. Catuaí Vermelho IAC-144 responded differentially to glyphosate drift depending on plant growth stage, regarding on photosynthesis, transpiration, stomatal conductance, and plant growth, in spite of macronutrient nutrition was not affected.Glifosato es el principal herbicida utilizado en las plantaciones de café (Coffea arabica L. en Brasil. Problemas con la deriva de herbicidas comúnmente ocurren en los campos de cultivo debido a condiciones no adecuadas de pulverización. Una serie de experimentos se llevó a cabo con el objetivo de evaluar la relación dosis-respuesta fisiológica de plantas de C. arabica expuestas a situaciones simuladas de exposición a deriva de glifosato en dos etapas distintas de crecimiento de las plantas. El glifosato se aplicó en dosis de 0

  5. Study of composition of espresso coffee prepared from various roast degrees of Coffea arabica L. coffee beans.

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    Kučera, Lukáš; Papoušek, Roman; Kurka, Ondřej; Barták, Petr; Bednář, Petr

    2016-05-15

    Espresso coffee samples prepared at various roasting degrees defined according to its basic conventional classification (light, medium, medium-dark and dark roasted) were analyzed by ultra-performance liquid chromatography/tandem mass spectrometry. Obtained raw data were processed using multivariate statistical analysis (Principal Component Analysis, PCA) to evaluate chemical differences between each roasting degrees (untargeted part of study). All four roasting degrees were resolved in appropriate Score plot. Orthogonal Projections to Latent Structures provided signals of significant markers describing the differences among particular roasting degrees. Detailed interpretation of those signals by targeted LC/MS(2) analysis revealed four groups of compounds. The first two groups involve chlorogenic acids and related lactones. The signals of other two sets of markers were ascribed to some specific atractylosides and particular melanoidins. Ratios of contents of selected representatives of each group to the sum of all identified markers were proposed as definite parameters for determination of roasting degree of Brazilian coffee Arabica. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Effects of shade on growth, production and quality of coffee (Coffea arabica) in Ethiopia

    OpenAIRE

    Bote, A.D.; Struik, P.C.

    2011-01-01

    The research work was conducted to evaluate the effect of shade on growth and production of coffee plants. To achieve this, growth and productivity of coffee plants growing under shade trees were compared with those of coffee plants growing under direct sun light. Different physiological, environmental and quality parameters were assessed for both treatments. Shade trees protected coffee plants against adverse environmental stresses such as high soil temperatures and low relative humidity. Sh...

  7. Effects of shade on growth, production and quality of coffee (Coffea arabica) in Ethiopia

    NARCIS (Netherlands)

    Bote, A.D.; Struik, P.C.

    2011-01-01

    The research work was conducted to evaluate the effect of shade on growth and production of coffee plants. To achieve this, growth and productivity of coffee plants growing under shade trees were compared with those of coffee plants growing under direct sun light. Different physiological,

  8. Volatile compounds profiles in unroasted Coffea arabica and Coffea canephora beans from different countries

    Directory of Open Access Journals (Sweden)

    Daniel KNYSAK

    Full Text Available Abstract Aroma is the most important factor in assessing the quality of coffee. The volatile compounds profile could be very important to confirm the authenticity of Coffea arabica. The study was carried out on two species of unroasted coffee beans: Coffea arabica from Colombia and Nepal and Coffea robusta from Uganda and Vietnam. Both Coffea arabica and Coffea canephora were imported to the country of analysis approximately 5 months prior to the research. Before the analysis, the coffee beans were kept in a sealed, dark container, at 21 °C. The tests were performed using an electronic nose. Its functioning is based on gas chromatography with two columns of different polarities in parallel and with 2 ultra sensitive Flame Ionization Detectors (FID. With multivariate statistics – Principal Components Analysis – it was possible to reduce the number of links and present them in two dimensions, which allowed for the unambiguous identification and assignment of samples to a particular species of coffee. By using an electronic nose, one can distinguish and group unroasted coffee beans’ flavours depending on the country of origin and species.

  9. The Antifungal Inhibitory Concentration Effectiveness Test From Ethanol Seed Arabica Coffee (Coffea arabica) Extract Against The Growth Of Candida albicans Patient Isolate With In Vitro Method

    Science.gov (United States)

    Satria Rakatama, Adam; Pramono, Andri; Yulianti, Retno

    2018-03-01

    Candida albicans are the most frequent cause of Vulvovaginalis Candidiasis infection. Its treatment using antifungal drugs, are oftenly caused side effects. The reduction of C.albicans growth and the reduction of antifungal drugs side effect, were our main purposed. Our study objective is determine the effectiveness of inhibitory power of arabica coffee seed ethanol extract on the growth of C.albicans patient isolates. The type of this research is experimental research. Kirby-bauer method with the Saboraud Dextrose Agar (SDA) media was used in this experiment. Inhibitory zone was observed around the disc, to determine the inhibitory power. The results showed that the inhibitory zone was formed on arabica coffee seed ethanol extract on 10%, 20%, 40%, and 80% concentration. Kruskal-Wallis test results (pthe concentration groups tested against the treatment group. The inhibitory zone was formed because of biochemical compound in arabica coffee seed such as caffeine, phenol, alkaloids, flavonoids, and saponins. Inhibitory zone in C.albicans patient isolates were smaller compared with C.albicans ATCC 90028 as gold standard. This showed that the virulence of C.albicans from patients isolates were higher. We concluded that arabica coffee seed ethanol extract could inhibiting the growth of C.albicans patient isolates. Optimization of coffee seed ethanol extract to obtain maximum active ingredients still needs to be done. This knowledge is expected to be used for the beginning manufacturer antifungal drug from natural product.

  10. Perfil sensorial da bebida café (Coffea arabica L. determinado por análise tempo-intensidade Sensorial profile of beverage coffee (Coffea arabica L. determined by analysis time-intensity

    Directory of Open Access Journals (Sweden)

    Marlene A. M. Monteiro

    2005-12-01

    Full Text Available O presente trabalho teve como objetivo avaliar o gosto amargo, sabor fermentado e sabor queimado de nove amostras (mole/clara, dura/clara, rio/clara, mole/expresso, dura/expresso, rio/expresso, mole/escura, dura/escura e rio/escura da bebida café (Coffea arabica L. por meio da análise Tempo-Intensidade (TI. Foram avaliados seis parâmetros da curva: tempo para atingir a intensidade máxima (TImáx, intensidade máxima do estímulo (Imáx, tempo correspondente ao ponto onde a intensidade máxima começa a declinar (Td, tempo de duração da intensidade máxima (Platô, área sob a curva (Área e tempo total de duração do estímulo (Ttot. A análise demonstrou que as amostras de torra escura tiveram maior intensidade máxima (Imáx e tempo de duração do estímulo (Ttot para gosto amargo e sabor queimado, sendo que as amostras de torra clara apresentaram menor intensidade destes estímulos. Em relação ao sabor fermentado, a amostra rio/expresso foi a que apresentou maior intensidade.The present work had as objective to evaluate the bitter taste, fermented flavor and burned flavor of nine samples (soft/light, hard/light, rio/light, soft/express, hard/express, rio/express, soft/dark, hard/dark and rio/dark of the drink coffee (Coffea arabica L. through the analysis Time-Intensity (TI. Six parameters of the curve were evaluated: time to reach the maximum intensity (TImáx, maximum intensity of the incentive (Imáx, time corresponding to the point where the maximum intensity begins to refuse (Td, time of duration of the maximum intensity (Plateau, area under the curve (Área and total time of duration of the incentive (Ttot. The analysis demonstrated that the samples of dark toast had larger maximum intensity (Imáx and time of duration of the incentive (Ttot for bitter taste and burned flavor, while the samples of light toast presented smaller intensity of these stimulus. For the fermented flavor, the sample rio/express was the one that

  11. Inter-genomic DNA Exchanges and Homeologous Gene Silencing Shaped the Nascent Allopolyploid Coffee Genome (Coffea arabica L.

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    Philippe Lashermes

    2016-09-01

    Full Text Available Allopolyploidization is a biological process that has played a major role in plant speciation and evolution. Genomic changes are common consequences of polyploidization, but their dynamics over time are still poorly understood. Coffea arabica, a recently formed allotetraploid, was chosen to study genetic changes that accompany allopolyploid formation. Both RNA-seq and DNA-seq data were generated from two genetically distant C. arabica accessions. Genomic structural variation was investigated using C. canephora, one of its diploid progenitors, as reference genome. The fate of 9047 duplicate homeologous genes was inferred and compared between the accessions. The pattern of SNP density along the reference genome was consistent with the allopolyploid structure. Large genomic duplications or deletions were not detected. Two homeologous copies were retained and expressed in 96% of the genes analyzed. Nevertheless, duplicated genes were found to be affected by various genomic changes leading to homeolog loss or silencing. Genetic and epigenetic changes were evidenced that could have played a major role in the stabilization of the unique ancestral allotetraploid and its subsequent diversification. While the early evolution of C. arabica mainly involved homeologous crossover exchanges, the later stage appears to have relied on more gradual evolution involving gene conversion and homeolog silencing.

  12. Avaliação da fertilidade dos solos de sistemas agroflorestais com cafeeiro (Coffea arabica L. em Lavras-MG Evaluation of soil fertility in agroforest systems with coffee trees (Coffea arabica L. in Lavras-MG

    Directory of Open Access Journals (Sweden)

    Bruno Grandi Salgado

    2006-06-01

    Full Text Available Com o objetivo de avaliar a fertilidade dos solos em um sistema agroflorestal composto por cafeeiros (Coffea arábica L. - Mundo Novo, ingazeiros (Inga vera Willd e grevíleas (Grevilea robusta A. Cunn, situado em Lavras, Minas Gerais, foi instalado o presente experimento. Usou-se um delineamento experimental inteiramente casualizado com três tratamentos e sete repetições. Os tratamentos foram: a cafeeiros a pleno sol, b cafeeiros consorciados com ingazeiros e c cafeeiros consociados com grevílea. Os espaçamentos dos cafeeiros nos três sistemas foi 4 x 1 m, para o ingazeiro 8 m x 15 m e para a grevílea 12 m x 10 m. Aos 15 anos de idade do cafeeiro e do ingazeiro e aos 9 anos da grevílea foram avaliadas as seguintes características dos solos, pH, acidez potencial (H+Al, alumínio trocável (Al+3, bases trocáveis (Ca+2 e Mg+2, potássio disponível (K+, fósforo disponível(P, enxofre (S, CTC efetiva (T, soma de bases (SB, saturação de bases (V e matéria orgânica (MO. Os resultados foram submetidos à analise de variância e as médias, comparadas pelo teste de Scott-Knott. Após a análise dos dados, concluiu-se que, embora tenha havido diferenças entre alguns elementos estudados, as características químicas dos solos nos três sistemas não foram severamente afetadas.The objective of this wark was to evaluat e the soil fertility in an agroforest system using coffee trees (Coffea arabica L - Mundo Novo, inga trees (Inga vera Wild and grevillea trees (Grevillea robusta A Cunn situated in Lavras, Minas Geris. A completely randomized experimental design with tree treatments and seven replicates was utilized. The treatments were : a coffee trees in full sunshine; b coffee trees mixed with inga trees and c coffee trees mixed with grevillea trees. Tree spacings in the three systems were 4 x 1m for coffee, 8 m x 15 m for inga and 12 x 10 m for grevillea. With coffee and inga trees at 15 years of age and grevillea trees at 9 years of age

  13. Contribution to the study of 14C-acetate as the precursor of aminoacids in detached leaves of coffee (Coffea arabica cv. Mundo Novo)

    International Nuclear Information System (INIS)

    Brasil, O.G.

    1975-01-01

    Labelled acetates with 14 C were used as the forerunner of aminoacids in leaves of coffee (Coffea arabica cv Mundo Novo). Leaves with the labelled acetates were incubated and released CO 2 was retained in paper discs with hiamine for further radioactivity detection. Separated proteins furnished 13 amino-acids through acid hidrolysis, all of them were identified by bidimensional filter paper chromatography. Through the obtained results it is possible to conclude that acetates are metabolized by the leafs and are related to the processes of leaf synthesis. It was possible to show that an utilization of acetate for energetical production via Krebs cycle was donne. The obtained conclusions show too that methylic carbon was more incorporated than carboxylic carbon [pt

  14. Spatial distribution of the coffee-leaf-miner (Leucoptera coffeella) in an organic coffee (Coffea arabica L.) field in formation

    OpenAIRE

    Scalon, João Domingos; Universidade Federal de Lavras; Freitas, Gabriela Alves; DEX/UFLA; Avelar, Maria Betania Lopes; DEX/UFLA; Zacarias, Mauricio Sérgio; EPAMIG/EcoCentro

    2011-01-01

    Coffee production has been one of the economy pillars of many tropical countries. Unfortunately, this crop is susceptible to infestation by the coffee-leaf-miner (Leucoptera coffeella (Guérin-Mèneville & Perrottet, 1842)) which causes severe damage to coffee plantations with losses that may reach 80% of the total production. In recent years, researchers have been trying to develop practices for minimizing the use of pesticides in the coffee-leaf-miner control. It is well known that the un...

  15. Compostos bioativos e atividade antioxidante do café (Coffea arabica L. Coffee (Coffea arabica L. bioactive compounds and antioxidant activity

    Directory of Open Access Journals (Sweden)

    Sheila Andrade Abrahão

    2010-04-01

    Full Text Available Conduziu-se este trabalho, com a proposta de avaliar o potencial antioxidante de dois padrões da bebida do café (rio e mole, verdes e torrados, utilizando modelos in vitro. Foram determinados o teor de fenólicos totais, ácido clorogênico (ácido 5-cafeoilquínico e cafeína das bebidas. A avaliação in vitro do potencial antioxidante foi investigada pelos métodos de captação do radical DPPH e pelo poder redutor de metais. Os dois padrões de bebida do café analisados não apresentaram diferenças quanto aos parâmetros cor, ácido clorogênico e cafeína. Observou-se que houve redução nos valores de ácido clorogênico à medida que os grãos foram torrados. O café verde bebida rio apresentou maior teor de fenólicos totais que o café bebida mole. Nos grãos torrados não foi observada diferença. A bebida do café independente da qualidade sensorial apresentou alto poder redutor e importante atividade sequestrante de radicais livres. A atividade sequestrante de radicais livres foi significativamente superior nas amostras obtidas a partir dos grãos torrados, quando comparados aos extratos dos grãos verdes. A torração, porém, reduziu o poder redutor das bebidas do café. Os dados obtidos permitem sugerir que, independente da classificação sensorial da bebida, o café apresenta expressiva capacidade sequestrante de radicais livres e poder redutor de metais.The present work intended to evaluate the antioxidant potential of two coffee sorts (soft and river, green and roasted, in vitro. Phenolic compounds content, chlorogenic acid (5-cafeoyolquinic and caffeine of the beverages were evaluated. In vitro evaluation of the antioxidant potential was investigated by DPPH radical scavenging assay and by reducing the power of metals. Both sorts of coffee did not present statistical differences for color, chlorogenic acid and caffeine. After roasting, 5-cafeoyolquinic acid levels decreased. River coffee beverage presented greater content

  16. Evaluation of biocontrol ability of native strains of Trichoderma spp on Rhizoctonia and Fusarium sp in coffee (Coffea arabica in experimental conditions

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    Nina Rudy

    2016-06-01

    Full Text Available Due to the indiscriminate use agrochemicals in conventional agriculture, it is causing pollution problems in the environment (soil, air and water, hence the search for alternatives that contribute to agricultural production by agro-chemical free sustainable production. This paper studies the biological control of damping off in coffee (Coffea arabica by applying antagonistic fungus Trichoderma sp. Under experimental conditions at laboratory facilities of the Academic Unit Carmen Pampa Campesina, a community of Carmen Pampa, Township Coroico. The aim of this study was to biologically control the "damping off", they found two genera that cause damping off in seedbed of coffee: Rhizoctonia sp. and Fusarium sp.To determine the percentage of growth and control in the culture medium, we used the method of counting quarters, where they gave the mycelial growth of antagonistic fungus Trichoderma sp., And the fungal pathogens Rhizoctonia sp. and Fusarium sp. Statistically there was a highly significant difference in the variable growth rate of Trichoderma sp. on pathogenic fungi Rhizoctonia sp. and Fusarium sp. at 3, 6 and 9 days that announces the time factor and treatments are interdependent. The control variable showed a highly significant difference in the time factor and treatment, but the interaction shows no significant difference this makes known factors that are independent, so the fungus Trichoderma sp. not depend on time in treatment, thus showing its inhibitory power to Rhizoctonia sp. and Fusarium sp .. This test gives references that there is antagonistic fungus control on the fungal pathogens Rhizoctonia sp. and Fusarium sp.

  17. Phytochemical Profile and in vitro Assessment of the Cytotoxicity of Green and Roasted Coffee Oils (Coffea arabica L. and their Polar Fractions

    Directory of Open Access Journals (Sweden)

    Ana Paula Lorenzen Voytena

    2018-03-01

    Full Text Available Green Coffea arabica L. seed oil (GCO has been used as an active cosmetic ingredient in many skin care products, due to its composition and balance of fatty acids. On the other hand, while roasted coffee oil (RCO is mainly used for imparting aroma in the food industry, there is no data available to suggest its safety in cell-based model systems. In this context, the present study aims to evaluate the chemical composition of GCO, RCO, and their correspondent polar fractions (PFs; and assess their cytotoxicity and antioxidant potential in vitro. RCO and RCO PF exhibited significantly higher amounts of phenolic compounds, when compared to both GCO and GCO PF. In the DPPH assay, after 5 min of incubation, RCO inhibited about 80% of radicals, while GCO only achieved half of this activity. Similar results were also obtained for their PFs. Upon exposure to GCO, no cytotoxic effects were observed, in fact, there were slight increments in cell proliferation. Nevertheless, cell exposure to RCO led to significant decreases in cell viability. Increases in the concentration of coffee oil PFs were associated with correspondent relevant increased cytotoxicity. Upon hydrogen peroxide-induced oxidative stress, neither GCO nor RCO treatment were effective in protecting cells.

  18. Integrating age in the detection and mapping of incongruous patches in coffee (Coffea arabica) plantations using multi-temporal Landsat 8 NDVI anomalies

    Science.gov (United States)

    Chemura, Abel; Mutanga, Onisimo; Dube, Timothy

    2017-05-01

    The development of cost-effective, reliable and easy to implement crop condition monitoring methods is urgently required for perennial tree crops such as coffee (Coffea arabica), as they are grown over large areas and represent long term and higher levels of investment. These monitoring methods are useful in identifying farm areas that experience poor crop growth, pest infestation, diseases outbreaks and/or to monitor response to management interventions. This study compares field level coffee mean NDVI and LSWI anomalies and age-adjusted coffee mean NDVI and LSWI anomalies in identifying and mapping incongruous patches across perennial coffee plantations. To achieve this objective, we first derived deviation of coffee pixels from the global coffee mean NDVI and LSWI values of nine sequential Landsat 8 OLI image scenes. We then evaluated the influence of coffee age class (young, mature and old) on Landsat-scale NDVI and LSWI values using a one-way ANOVA and since results showed significant differences, we adjusted NDVI and LSWI anomalies for age-class. We then used the cumulative inverse distribution function (α ≤ 0.05) to identify fields and within field areas with excessive deviation of NDVI and LSWI from the global and the age-expected mean for each of the Landsat 8 OLI scene dates spanning three seasons. Results from accuracy assessment indicated that it was possible to separate incongruous and healthy patches using these anomalies and that using NDVI performed better than using LSWI for both global and age-adjusted mean anomalies. Using the age-adjusted anomalies performed better in separating incongruous and healthy patches than using the global mean for both NDVI (Overall accuracy = 80.9% and 68.1% respectively) and for LSWI (Overall accuracy = 68.1% and 48.9% respectively). When applied to other Landsat 8 OLI scenes, the results showed that the proportions of coffee fields that were modelled incongruent decreased with time for the young age category and

  19. Selection of Arabica coffee types resistant to coffee berry disease in Ethiopia

    NARCIS (Netherlands)

    Graaff, van der N.A.

    1981-01-01

    Descriptive part. A review is given of: the importance of Coffea arabica to Ethiopia; coffee research; habitus, origin and cultivation of C. arabica ; theoretical aspects of resistance and its implications for the system C.

  20. Storage of soil organic carbon in coffee (Coffea arabica L. production systems in the municipality of Líbano, Tolima, Colombia

    Directory of Open Access Journals (Sweden)

    Hernán Jair Andrade Castañeda

    2013-07-01

    Full Text Available The increase in greenhouse gas emissions from anthropogenic sources has resulted in climate change, which affects all living beings. Coffee (Coffea arabica L. plantations, in monoculture or together with timber species such as salmwood (Cordia alliodora, mitigate climate change due to fixation of atmospheric CO2 that is deposited in biomass and soils. This study was carried out in the municipality of Líbano, Tolima, Colombia with the objective of defining which of the following coffee production systems store more soil organic carbon (SOC: 1 monoculture, 2 agroforestry systems (AFS with salmwood, and 3 AFS with plantain. Farms with those systems that are the most dominant in the study zone were selected. From each system, five repetitions were identified to be analyzed with a completely randomized design. In each plot or repetition, five samples for bulk density (BD using the cylinder method and a composite sample for concentration of SOC were taken and analyzed using the Walkley and Black approach. The systems of production did not significantly (p > 0.05 affect either the BD or the concentration of SOC. However, AFS with plantain tended to have less BD than monoculture and AFS with salmwood (0.83 ± 0.03 vs 0.88 ± 0.03 vs 0.92 ± 0.04 g·cm-3, respectively. These systems of production stored between 50 and 54 t·ha-1 of SOC in the top 30 cm, which indicates their capacity for climate change mitigation.

  1. Optimization of coffee (Coffea arabica transformation parameters using uidA and hpt genes: effect of osmotic pre-treatment, helium pressure and target distance

    Directory of Open Access Journals (Sweden)

    Andrés M Gatica

    2009-11-01

    Full Text Available The aim of this work was to optimize the biolistic delivery parameters that affect the DNA delivery and stable expression of marker genes into coffee tissues (Coffea arabica. L. cvs. Caturra and Catuaí. The effect of osmotic preculture length, osmotic concentration of medium, Helium pressure and target distance on transient expression of the uidA gene in coffee leaves and somatic embryos were tested. The highest transient uidA expression was obtained when Caturra (18.3±2.8 and Catuaí (6.8±2.0 leaves and Catuaí embryos (80.0±7.4 were cultured for 5h on Yasuda medium complemented with 0.5M Mannitol +0.5M Sorbitol. The combination of 1100psi and a target distance of 9cm resulted in the highest number of blue spots per Caturra leaf segment (23.6±3.9, whereas for the Catuaí variety the combination of 1100psi and a target distance of six (10.2±1.9 and nine (8.2±1.9 cm gave the highest number of blue spots per leaf segment. The optimized protocol was tested with pCAMBIA 1 301 (uidA gene and the hpt gene, pCAMBIA 1 305.2 (uidA version GUSPlus ™ and the hpt gene and pCAMBIA 1 301-BAR (uidA gene and the bar gene. The highest number of blue spots was obtained when Caturra (54.6±5.7 and Catuaí (28.9±4.3 leaves were bombarded with pCAMBIA 1 305.2. Selection of bombarded coffee tissues with 100mg/l hygromicyn caused the oxidation of tissues. Rev. Biol. Trop. 57 (Suppl. 1: 151-160. Epub 2009 November 30.La presente investigación tuvo como objetivo optimizar los parámetros que afectan la incorporación y expresión de genes marcadores mediante biobalística en segmentos de hoja y embriones somáticos de café (Coffea arabica. L. cvs. Caturra y Catuaí. La mayor expresión transitoria del gen uidA en segmentos de hoja de Caturra (18.3±2.8 y Catuaí (6.8±2.0 y embriones somáticos de Catuaí (80.0±7.4 se obtuvo al cultivar los explantes por cinco horas previo al bombardeo en el medio Yasuda complementado con 0.5M mannitol+0.5M sorbitol

  2. Detection of corn adulteration in Brazilian coffee (Coffea arabica) by tocopherol profiling and near-infrared (NIR) spectroscopy

    Science.gov (United States)

    Coffee is a high-value commodity that is a target for adulteration, especially after the beans have been roasted and ground. Countries such as Brazil, the second largest coffee producer, have set limits on the allowable amount of coffee contamination and adulteration. Therefore, there is significant...

  3. Quality of the surface of Coffea arabica wood

    Directory of Open Access Journals (Sweden)

    Pedro Paulo de Carvalho Braga

    2014-03-01

    Full Text Available The wood of Coffea arabica L. is considered a a residue of the coffee industry and is widely used as a source of energy. Few studies have shown other destinations such as the manufacture of small objects and furniture with rustic design. The objective of this work was to find the best fit in cutting speed during machining planer trowel the wood of Coffea arabica, taking into consideration the quality of the machined surface. The wood from the Coffea arabica came from an 15 years planting, spacing 3 x 2 m, of the municipality of Machado / MG. The tree was pruned, unfolded and flattened, in order for getting cut-proof of 30 mm thick, with variables length and width. The machining tests were performed at the Laboratory of Wood Machining (DCF / UFLA, varying the cutting speed in plane trowel. The qualification of the machined surface was performed by the feed per tooth (fz, visual analysis (ASTM D 1666-87 and roughness Ra and Rz. It was used a completely randomized design with 30 repetitions. We conducted the analysis of variance test and the average of Scott-Knott, at 5% significance level. It was calculated the percentage of marks obtained for the feed per tooth. The results showed that the quality of machined surface with cutting speeds of 19 and 21 m∙s-1 and forward speed of 6 m∙min-1 were satisfactory with small surveys of fiber and low values of feed per tooth ( fz and roughness Ra and Rz.

  4. The use of the shaded coffee (sombrío del cafeto production thecnique (Coffea arabica in colombia

    Directory of Open Access Journals (Sweden)

    Gonzalo Enrique Mancilla Díaz

    2012-06-01

    Full Text Available The art of coffee production under shade has been one of the most effective methods used by our ancestors in order to adapt the coffee trees to precarious conditions, where they would not otherwise produce profitably due to the conditions of elevation, light, water availability, percentage of organic matter, temperature and other climatic and geographic factors. The objective of this study was to explain in a consistent and clear way the interaction of different effects generated in the soil, atmosphere and the crop, as a result of the use of the shaded coffee technique (sombrío de café in coffee plantations. This provides tools that are applicable to the Colombian coffee growing industry, which can produce an improvement in the use of this technique, based on the zoning of the productive unit. This will generate an increase in the life and productivity of the crop.

  5. Caffeine inheritance in interspecific hybrids of Coffea arabica x Coffea canephora (Gentianales, Rubiaceae

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    Regina H.G. Priolli

    2008-01-01

    Full Text Available Caffeine inheritance was investigated in F2 and BC1F1 generations between Coffea arabica var. Bourbon Vermelho (BV and Coffea canephora var. Robusta 4x (R4x. The caffeine content of seeds and leaves was determined during 2004 and 2005. Microsatellite loci-markers were used to deduce the meiotic pattern of chromosome pairing of tetraploid interspecific hybrids. Genetic analysis indicated that caffeine content in seeds was quantitatively inherited and controlled by genes with additive effects. The estimates of broad-sense heritability of caffeine content in seeds were high for both generations. In coffee leaves, the caffeine content (BSH from the same populations showed transgressive segregants with enhanced levels and high BSH. Segregation of loci-markers in BC1F1 populations showed that the ratios of the gametes genotype did not differ significantly from those expected assuming random associations and tetrasomic inheritance. The results confirm the existence of distinct mechanisms controlling the caffeine content in seeds and leaves, the gene exchange between the C. arabica BV and C. canephora R4x genomes and favorable conditions for improving caffeine content in this coffee population.

  6. Mapping spatial variability of foliar nitrogen in coffee (Coffea arabica L.) plantations with multispectral Sentinel-2 MSI data

    Science.gov (United States)

    Chemura, Abel; Mutanga, Onisimo; Odindi, John; Kutywayo, Dumisani

    2018-04-01

    Nitrogen (N) is the most limiting factor to coffee development and productivity. Therefore, development of rapid, spatially explicit and temporal remote sensing-based approaches to determine spatial variability of coffee foliar N are imperative for increasing yields, reducing production costs and mitigating environmental impacts associated with excessive N applications. This study sought to assess the value of Sentinel-2 MSI spectral bands and vegetation indices in empirical estimation of coffee foliar N content at landscape level. Results showed that coffee foliar N is related to Sentinel-2 MSI B4 (R2 = 0.32), B6 (R2 = 0.49), B7 (R2 = 0.42), B8 (R2 = 0.57) and B12 (R2 = 0.24) bands. Vegetation indices were more related to coffee foliar N as shown by the Inverted Red-Edge Chlorophyll Index - IRECI (R2 = 0.66), Relative Normalized Difference Index - RNDVI (R2 = 0.48), CIRE1 (R2 = 0.28), and Normalized Difference Infrared Index - NDII (R2 = 0.37). These variables were also identified by the random forest variable optimisation as the most valuable in coffee foliar N prediction. Modelling coffee foliar N using vegetation indices produced better accuracy (R2 = 0.71 with RMSE = 0.27 for all and R2 = 0.73 with RMSE = 0.25 for optimized variables), compared to using spectral bands (R2 = 0.57 with RMSE = 0.32 for all and R2 = 0.58 with RMSE = 0.32 for optimized variables). Combining optimized bands and vegetation indices produced the best results in coffee foliar N modelling (R2 = 0.78, RMSE = 0.23). All the three best performing models (all vegetation indices, optimized vegetation indices and combining optimal bands and optimal vegetation indices) established that 15.2 ha (4.7%) of the total area under investigation had low foliar N levels (landscape scale.

  7. Differentially Accumulated Proteins in Coffea arabica Seeds during Perisperm Tissue Development and Their Relationship to Coffee Grain Size.

    Science.gov (United States)

    Alves, Leonardo Cardoso; Magalhães, Diogo Maciel De; Labate, Mônica Teresa Veneziano; Guidetti-Gonzalez, Simone; Labate, Carlos Alberto; Domingues, Douglas Silva; Sera, Tumoru; Vieira, Luiz Gonzaga Esteves; Pereira, Luiz Filipe Protasio

    2016-02-24

    Coffee is one of the most important crops for developing countries. Coffee classification for trading is related to several factors, including grain size. Larger grains have higher market value then smaller ones. Coffee grain size is determined by the development of the perisperm, a transient tissue with a highly active metabolism, which is replaced by the endosperm during seed development. In this study, a proteomics approach was used to identify differentially accumulated proteins during perisperm development in two genotypes with regular (IPR59) and large grain sizes (IPR59-Graudo) in three developmental stages. Twenty-four spots were identified by MALDI-TOF/TOF-MS, corresponding to 15 proteins. We grouped them into categories as follows: storage (11S), methionine metabolism, cell division and elongation, metabolic processes (mainly redox), and energy. Our data enabled us to show that perisperm metabolism in IPR59 occurs at a higher rate than in IPR59-Graudo, which is supported by the accumulation of energy and detoxification-related proteins. We hypothesized that grain and fruit size divergences between the two coffee genotypes may be due to the comparatively earlier triggering of seed development processes in IPR59. We also demonstrated for the first time that the 11S protein is accumulated in the coffee perisperm.

  8. A flórula invasora da cultura do café (Coffea arabica L. no Estado de Minas Gerais, Brasil Weeds in coffee (Coffea arabica L. plantations in the state of Minas Gerais, Brazil

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    Manuel Losada Gavilanes

    1988-01-01

    Full Text Available Nas áreas de cultura de café (Coffea arábica L., no Estado de Minas Gerais, foram coletadas e identificadas 388 espécies de plantas invasoras (= plantas daninhas, pertencentes a 51 famílias botânicas, representando 182 gêneros, sendo que as famílias Compositae, Gramineae, Leguminosae, Malvaceae, Solanaceae, Euphorbiaceae, Rubiaceae, Amaranthaceae, Convolvulaceae e Verbenaceae, são as mais importantes em relação à cultura. As plantas coletadas, devidamente etiquetadas e identificadas, foram anexadas, parte delas no PAMG (Herbário da EPAMIG, Belo Horizonte, MG e, a outra parte, no Herbarium ESAL (Herbário do Departamento de Biologia da Escola Superior de Agricultura de Lavras - ESAL, Lavras - MG.A survey in the cultivation area of coffee in the State of Minas Gerais, Brazil, has resulted in the determination of 388 weed species, of 182 genera belonging to 51 families; the families presenting a greater number of espécies are: Compositae, Leguminosae, Gramineae, Malvaceae, Solanaceae, Rubiaceae, Convolvulaceae, Euphorbiaceae, Amaranthaceae and Verbenaceae with 65, 48, 42, 30, 19, 17, 16, 14, 12, 10 species, respectively.

  9. Identification of novel and conserved microRNAs in Coffea canephora and Coffea arabica

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    Guilherme Loss-Morais

    2014-12-01

    Full Text Available As microRNAs (miRNAs are important regulators of many biological processes, a series of small RNAomes from plants have been produced in the last decade. However, miRNA data from several groups of plants are still lacking, including some economically important crops. Here microRNAs from Coffea canephora leaves were profiled and 58 unique sequences belonging to 33 families were found, including two novel microRNAs that have never been described before in plants. Some of the microRNA sequences were also identified in Coffea arabica that, together with C. canephora, correspond to the two major sources of coffee production in the world. The targets of almost all miRNAs were also predicted on coffee expressed sequences. This is the first report of novel miRNAs in the genus Coffea, and also the first in the plant order Gentianales. The data obtained establishes the basis for the understanding of the complex miRNA-target network on those two important crops.

  10. ABA Inhibits Embryo Cell Expansion and Early Cell Division Events During Coffee (Coffea arabica 'Rubi') Seed Germination

    NARCIS (Netherlands)

    Silva, da E.A.A.; Toorop, P.E.; Lammeren, van A.A.M.; Hilhorst, H.W.M.

    2008-01-01

    Background and Aims: Coffee seed germination represents an interplay between the embryo and the surrounding endosperm. A sequence of events in both parts of the seed determines whether germination will be successful or not. Following previous studies, the aim here was to further characterize the

  11. Teste de condutividade elétrica individual na avaliação da qualidade fisiológica de sementes de café (Coffea arabica L. Individual electrical conductivity test for evaluation of the physiological quality of coffee seeds (Coffea arabica L.

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    Paula de Souza Cabral Costa

    2006-02-01

    Full Text Available O teste de condutividade elétrica é usado para medir os exsudatos das sementes, que certamente refletem a integridade do sistema de membranas. O teste é rápido e de operação simples, mas a metodologia necessita ser testada para cada espécie individualmente para obter uma melhor precisão e exatidão dos resultados. Este trabalho foi conduzido visando determinar o ponto de partição na realização do teste de condutividade elétrica em sementes de café. Dez lotes de sementes de Coffea arabica sem pergaminho, cultivar Acaiá foram usados nesse estudo. Para a avaliação do ponto de partição as sementes foram embebidas por 96 horas e submetidas ao teste de germinação, correlacionando os valores de condutividade observados com os valores obtidos no teste de germinação. O ponto de partição é de 120,5µS.cm-1, considerando 70% de germinação.The conductivity test is used to measure the leaches from the seeds, which certaintily reflect the membrane system integrity. The test is rapid and it is of simple operation, but the methodology needs to be tested for each individual species in order to reach a better precision and accuracy. This work was conducted to determinate the partition point for the realization of electrical conductivity test in coffee seeds. Ten seed lots of Coffea arabica, cultivar Acaiá, without parchment, were used in this study. For the evaluation of the partition point, seeds were imbibed for 96 hours and submitted to the germination test, with the correlation between the conductivity values and those obtained in the germination test being evaluated. The partition point is 120,5µS.cm-1, considering 70% of germination.

  12. Inhibitory effect of Coffea arabica bean in testosterone induced prostatic hyperplasia in Sprague-Dawley rats

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    Kristian Alfonso G. Cueto

    2016-05-01

    Full Text Available Benign prostatic hyperplasia (BPH has been described as the uncontrolled prostate gland growth which leads to difficulty in urination. One of the treatment of BPH is saw palmetto lipid extracts which has been shown to inhibit prostate 5 α-reductase and some of its components (lauric acid, myristic acid and oleic acid also inhibit the enzyme. Coffee was also rich in fatty acids namely linoleic acid, oleic acid and palmitic acid. The aim of this research is to investigate whether coffee is effective in preventing testosterone-induced prostatic hyperplasia in rats using testosterone propionate and estradiol valerate. After and before the induction, the rats were tested for prostate specific antigen (PSA . The condition of the prostate gland of the test animals were correlated with the results of the said test and in the histopathologic results. After 14 days of experimentation, animals in the test group significantly decreased their PSA levels as compared to the BPH group. The histomorphology showed that Coffea arabica bean oil inhibited testosterone propionate while estradiol valerate induced prostatic hyperplasia. These findings indicate that Coffee arabica bean oil effectively inhibited the development of BPH. With the proven safety of coffee oil, these findings strongly support the feasibility of using Coffea arabica bean oil therapeutically in treating BPH.

  13. Melhoramento do cafeeiro: XLII. Produtividade de progênies derivadas de hibridação dos cultivares Laurina e Mundo Novo Coffee breeding: XLII. Yield of progenies from crosses of Laurina and Mundo Novo cultivars of Coffea arabica L.

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    Alcides Carvalho

    1988-01-01

    Full Text Available O cultivar Laurina de Coffea arabica L. caracteriza-se pelo pequeno porte, folhas de dimensões reduzidas, frutos afilados na base, sementes pequenas e afiladas, pequeno rendimento e reduzida produção. Apresenta, no entanto, bebida de boa qualidade e baixo teor de cafeína nas sementes. Suas principais características são controladas pela ação de um par de alelos recessivos lrlr, de acentuado efeito pleiotrópico. Devido ao atual interesse do comércio por produto de baixo teor de cafeína, iniciaram-se pesquisas tendo em vista principalmente aumentar a produtividade do 'Laurina'. Para esse fim, realizaram-se numerosas hibridações de cafeeiros do 'Laurina' com os do 'Mundo Novo' (Coffea arabica e, posteriormente, retrocruzamentos com o 'Mundo Novo'. Estudaram-se as progênies F2 e retrocruzamentos com o 'Mundo Novo' (RC em Campinas, em um experimento, anotando-se as produções por oito anos consecutivos. Separaram-se algumas progênies F2 em dois grupos, antes do plantio: normais (LrLr,Lrlr e laurina (Irlr. Como testemunhas, usaram-se progênies do 'Mundo Novo' e 'Catuaí Amarelo' de C. arabica. O conjunto de plantas F2 do grupo laurina e os retrocruzamentos tiveram produção média maior do que as plantas F2 normais, porém menor do que as testemunhas. Alguns retrocruzamentos e progênies F2 apresentaram plantas com razoável produtividade, indicando que, através de retrocruzamentos com o 'Mundo Novo', podem-se obter novos tipos comerciais com as características morfológicas do 'Laurina'. Fizeram-se considerações sobre a melhor capacidade de combinação do 'Laurina' com algumas seleções do 'Mundo Novo'.The Laurina cultivars of Coffea arabica L. has a reduced plant size, small leaves, small and pointed seeds and low yield capacity. However the seeds have a good cup quality and the desirable characteristic of low caffeine content The Laurina phenotype is supposed to be controlled by a pair of recessive alleles lrlr, with

  14. [Establisment of a genetic transformation method of coffee (Coffea arabica cv. Catimor) and incorporation of bar gene for ammonium glufosinate resistance].

    Science.gov (United States)

    Fernández Da Silva, Rafael

    2003-01-01

    In order to establish a successful method of genetic transformation in Coffea arabica cv. Catimor, different conditions of generation and electroporation were evaluated on different plant tissues. Cell suspension system was improved using one hormone only (BA), obtaining high yields of primary and secondary somatic embryo production. For selection of viable and potentially transformed cells, MTT (1%) method and ammonium glufosinate concentration (1 mg/L in leaf, callus and embryos; and 5 mg/L in cells) were established. Different conditions were evaluated to electroporate different explants (embryogenic callus, vitroplants leaves, globular and torpedo embryos). The highest gus gene expression percentage by explant were found on enzymatic treated tissues at 375 V/cm in callus, and at 625 V/cm in leaves and embryos. Torpedo embryos cultured on liquid medium were the only type of tissue that could regenerate into plants, where secondary somatic embryos were obtained. Those embryos were positive to the gus gene histochemical test and to the gus and bar genes amplification on a PCR reaction.

  15. Melhoramento do cafeeiro: XXXVIII. Observações sobre progênies do cultivar Mundo-Novo de Coffea arabica na estação experimental de Mococa Coffee breeding: XXXVIII-observation on progenies of the Mundo-Novo cultivars of Coffea arabica in the Mococa experimental station

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    Túlio R. Rocha

    1980-01-01

    Full Text Available Os dados analisados no experimento localizado em Mococa sobre a produtividade de 112 progênies dos cultivares Mundo-Novo S1 e S2, Bourbon-Amarelo, BourbonVermelho e Caturra-Vermelho de Coffea arabica no período de 1955 a 1971, indicaram que as de Mundo-Novo S1, de prefixos MP 474, MP 502, MP 469, MP 492 e MP 475, revelaram-se como as mais produtivas, assemelhando-se a algumas progênies 'Mundo--Novo' S2. Dentre estas, destacou-se a de prefixo MP 388-6, que atingiu o nível mais elevado de produção do experimento. As progênies de 'Mundo-Novo', em conjunto, produziram 44% a mais do que as de Bourbon-Amarelo e, estas, 60% a mais do que as de Bourbon-Vermelho e Caturra-Vermelho. A altura e o diâmetro da copa atingiram valores médios mais elevados para as progênies de 'Mundo-Novo'. Verificaram-se correlações positivas e altamente significativas entre altura média da planta e diâmetro médio da copa com a produção das progênies. As progênies mais produtivas revelaram rendimento (relação entre peso de café maduro e beneficiado de aproximadamente 6,0 e porcentagem de sementes normais, do tipo chato, acima de 80. Quanto ao tamanho das sementes do tipo chato, duas progênies 'Mundo-Novo' S1, MP 474 e MP 452, apresentaram peneira média maior, permi-tindo seleção de plantas com essa característica e com elevada produção.Coffee progenies of the Mundo-Novo cultivars of Coffea arabica were studied in an experiment located at the Mococa Experimental Station of the Instituto Agronômico in comparison with Bourbon-Amarelo, Bourbon-Vermelho and Caturra-Vermelho cultivars of the same species. During a period of 17 consecutive cropping years (1955-1971, Mundo-Novo yielded approximately 44% more than Bourbon-Amarelo and this cultivars yielded 60% more than Bourbon-Vermelho and Caturra-Vermelho. Among the 89 S1 'Mundo-Novo' progenies, MP 474, MP 502, MP 469, MP 492 and MP 475 yielded as much as the two best 'Mundo-Novo' S2 progenies. Greater

  16. Coffee seed physiology

    NARCIS (Netherlands)

    Eira, M.T.S.; Silva, da E.A.A.; Castro, de R.D.; Dussert, S.; Walters, C.; Bewley, J.D.; Hilhorst, H.W.M.

    2006-01-01

    Coffee is a member of the Rubiaceae family and the genus Coffea. There are more than 70 species of coffee but only two are economically important: Coffea arabica L. and Coffea canephora Pierre; 70 % of the coffee traded in the world is arabica and 30 % is robusta (C. canephora). Other species such

  17. Redistribution of the solar radiation and the rain inside of coffee plantations (Arabic Coffea L.)

    International Nuclear Information System (INIS)

    Jaramillo Robledo, Alvaro

    2005-01-01

    The following review presents a series of studies on microclimates of non-shaded and shaded conditions of coffee plantations (Coffea arabica L.) in Colombia. Likewise, The redistribution of solar radiation and the temperature, as well as the energy balance, of the coffee plant and the crop are described. The results on the components of water balance and transport of nutrients within the coffee plantations are reported

  18. Microscopia eletrônica de varredura do endosperma de café (Coffea arabica L. durante o processo de secagem Scanning electron microscopy of the endosperm of coffee (Coffea arabica L. during the drying process

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    Reni Saath

    2010-02-01

    Full Text Available A manutenção da integridade das membranas celulares, entre outros eventos, é um forte indicativo de que a qualidade do café foi preservada na pós-colheita. Objetivou-se neste trabalho, analisar o efeito de diferentes métodos de secagem na manutenção da integridade da parede celular e da membrana plasmática de café natural e café despolpado, buscando determinar as condições e o momento em que ocorrem as rupturas microscópicas. Os cafés foram submetidos a um período de pré-secagem em terreiro. Após este, uma parcela de cada tipo de café foi desidratada no terreiro e, outra, à temperatura de 40ºC e 60ºC em secadores de camada fixa, monitorando-se a temperatura e o teor de água até 11% (bu. Nesse período, grãos foram aleatoriamente amostrados e fragmentos do endosperma preparados para a microscopia eletrônica de varredura, registrando-se diversas eletromicrografias, avaliando-se as alterações na membrana plasmática da célula do endosperma dos grãos de cafés em função do teor de água e tempo de secagem. O citoplasma das células a 11% (bu de teor de água não foi comprometido na secagem em terreiro e a 40°C; na secagem a 60°C, observou-se comprometimento nas estruturas celulares nos cafés com teor de água de 20% (bu.The maintenance of the integrity of cellular membranes, among other events, is a strong indicator that the quality of the coffee was preserved in the post-harvesting process. Therefore, this work aimed to analyze the effect of different drying methods on the maintenance of the integrity of cell walls and plasma membrane of natural and de-pulped coffee in order to determine the conditions and the moment that microscopic ruptures take place. The coffee was submitted to a pre-drying period on a concrete patio. After this, a sample of each type of coffee was dehydrated outdoors and another, with heated air at 40ºC and 60ºC in fixed-layer dryers, controlling the grain temperature and the moisture

  19. Crescimento vegetativo e produção de cafeeiros (Coffea arabica L. recepados em duas épocas, conduzidos em espaçamentos crescentes Vegetative growth and yield of coffee plants (Coffea arabica L. in two different pruning times, conducted at different spacings

    Directory of Open Access Journals (Sweden)

    Sérgio Parreiras Pereira

    2007-06-01

    Full Text Available O experimento foi instalado na Fazenda Experimental da Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG em Machado, Sul de Minas Gerais, em 1992, com o objetivo de avaliar as conseqüências da redução dos espaçamentos entre as linhas e entre as plantas na linha de plantio sobre a produção e a fenologia do cafeeiro(Coffea arabica L.. O delineamento experimental foi o blocos casualizados - DBC, em um arranjo fatorial 4 x 3 com parcela subdividida, sendo quatro distâncias entre as linhas (2,0; 2,5; 3,0 e 3,5 m e três distâncias entre as plantas na linha de plantio (0,5; 0,75; 1,0 m, e duas épocas de poda (uma precoce feita logo após a colheita em de julho 2002 e a outra tardia em de janeiro de 2003, totalizando 24 tratamentos com três repetições. Em julho de 2002 e em janeiro de 2003 foi realizada a poda tipo "recepa", na qual foram conduzidas duas brotações por planta. Em agosto de 2004, avaliou-se o crescimento dos componentes vegetativos e a produção das parcelas. Os espaçamentos adotados não influenciaram o crescimento de nenhum dos componentes vegetativos das brotações no período avaliado. Todas as características vegetativas foram influenciadas positivamente pela adoção da poda precoce, assim como a produtividade da primeira colheita realizada após a poda, que foi também influenciada positivamente pela adoção de espaçamentos mais adensados. Os cafeeiros que foram submetidos à poda tardia não produziram, em julho de 2004, como aqueles podados precocemente.The experiment was established in the Empresa de Pesquisa Agropecuária de Minas Gerais - Epamig Experiment Station, located in the city of Machado, south of the Minas Gerais state, Brazil, in the year of 1992, with the objective of evaluating the consequences of the reduction on planting spaces among rows and among plants, upon beans yield and plant phenology (Coffea arabica L.. The experimental design used was a 4x 3 factorial with split plot at

  20. Influência de diferentes sistemas de colheita na qualidade do café (Coffea arabica L. Influence of different harvest systems on quality of coffee (Coffea arabica L.

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    Cássio de Carvalho Júnior

    2003-10-01

    Full Text Available Com o objetivo de avaliar a influência da colheita na qualidade do café, foram estudados seis diferentes sistemas. O experimento foi conduzido na fazenda Rancho Fundo, município de Campos Gerais, MG, no Departamento de Ciência dos Alimentos da UFLA e no Centro Tecnológico do Sul de Minas da EPAMIG. Em uma lavoura da cultivar Acaiá Cerrado, foram sorteadas, ao acaso, dezoito parcelas com trinta metros de comprimento. A colheita foi realizada quando a lavoura apresentava aproximadamente 20% de frutos verdes. Um terço do café derriçado de cada parcela era formado pela mistura de frutos provenientes da lavoura. O restante do café foi lavado e separado em frutos-bóia e verde/cereja. Os diferentes tipos de café foram colocados no terreiro de secagem até atingirem o teor de água de 11% (b.u.. Após a secagem, o café foi beneficiado e submetido às seguintes avaliações: polifenóis, açúcares totais, redutores e não-redutores, sólidos solúveis totais, acidez titulável total e prova de xícara. Analisando os resultados obtidos, apesar de terem sido observadas diferenças significativas entre os valores médios de polifenóis, açúcares, sólidos solúveis e acidez titulável para os diferentes sistemas de colheita, não foi possível estabelecer uma associação definida entre sistema de colheita e composição química. Não foi possível também distinguir, a partir da prova de xícara, diferenças na qualidade do café em função do sistema de colheita, pois, todas as amostras analisadas apresentaram bebida classificada como mole, apenas mole e dura.Aiming to evaluating the influence of the harvest on coffee quality, six harvest systems with different mechanization level were studied. The present work was developed on the Rancho Fundo farm, Campos Gerais town, MG, in the Food Science Department of the UFLA and in the “Centro Tecnológico do Sul de Minas” of EPAMIG. In a crop of the Acaiá Cerrado cultivars were randomized

  1. Physical Land Suitability for Civet Arabica Coffee: Case Study of Bandung and West Bandung Regencies, Indonesia

    Science.gov (United States)

    Chairani, E.; Supriatna, J.; Koestoer, R.; Moeliono, M.

    2017-12-01

    Indonesia has been widely known as the best Arabica coffee (Coffea arabica) producer, in terms of both aspects, quality and number of product. Currently, its production, however, declines to the 3rd rank internationally. Issues emerged in the coffee cultivation are: land unsuitability, low quality of seeds, and poor management. Among Arabica coffee types, wild civet coffee is the most expensive one and harvested from the coffee beans which have been digested naturally. The study aims to determine the physical suitability of land as well as the constraints related to land for civet Arabica coffee in selected study cases, e.g., Bandung and Bandung Barat. The research methods employ multi-criteria analysis, and combined with weighted overlaying techniques for mapping. The criteria include temperature, rainfall, humidity, duration of dry season, slope, altitude, type of soil, soil texture, and erosion potential. Parameters of civet (Paradoxurus hermaphroditus) are land use, altitude, and temperature. Local policy strongly supports the extensive management for land and the increase of coffee export. Processing data involved matching the comparison between guideline requirements for the land suitability classes, characteristics of Arabica coffee and civet habitat. The results covered the profile suitable land of the civet Arabica coffee in the study areas.

  2. Quantification of Coffea arabica and Coffea canephora var. robusta concentration in blends by means of synchronous fluorescence and UV-Vis spectroscopies.

    Science.gov (United States)

    Dankowska, A; Domagała, A; Kowalewski, W

    2017-09-01

    The potential of fluorescence, UV-Vis spectroscopies as well as the low- and mid-level data fusion of both spectroscopies for the quantification of concentrations of roasted Coffea arabica and Coffea canephora var. robusta in coffee blends was investigated. Principal component analysis was used to reduce data multidimensionality. To calculate the level of undeclared addition, multiple linear regression (PCA-MLR) models were used with lowest root mean square error of calibration (RMSEC) of 3.6% and root mean square error of cross-validation (RMSECV) of 7.9%. LDA analysis was applied to fluorescence intensities and UV spectra of Coffea arabica, canephora samples, and their mixtures in order to examine classification ability. The best performance of PCA-LDA analysis was observed for data fusion of UV and fluorescence intensity measurements at wavelength interval of 60nm. LDA showed that data fusion can achieve over 96% of correct classifications (sensitivity) in the test set and 100% of correct classifications in the training set, with low-level data fusion. The corresponding results for individual spectroscopies ranged from 90% (UV-Vis spectroscopy) to 77% (synchronous fluorescence) in the test set, and from 93% to 97% in the training set. The results demonstrate that fluorescence, UV, and visible spectroscopies complement each other, giving a complementary effect for the quantification of roasted Coffea arabica and Coffea canephora var. robusta concentration in blends. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Métodos de controle de plantas invasoras na cultura do cafeeiro (Coffea arabica L. e componentes da acidez do solo Weed control methods and soil acidity components in coffee plantation (Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    Elifas Nunes Alcântara

    2007-12-01

    Full Text Available Em lavouras perenes, como na cultura do cafeeiro, o controle de plantas invasoras tem sido feito por meio de métodos manuais, mecanizados, químicos e associações destes. De modo geral, têm-se avaliado os diferentes métodos sob o ponto de vista de eficiência e de custo no controle das plantas invasoras; no entanto, a influência deles sobre as condições químicas do solo, praticamente, não tem sido estudada, principalmente a longo prazo. O objetivo deste estudo foi avaliar o efeito de diferentes métodos de controle de plantas invasoras na cultura do cafeeiro sobre os componentes da acidez de um Latossolo Vermelho distroférrico da região de São Sebastião do Paraíso, MG. Sete tratamentos de controle de plantas invasoras foram avaliados: roçadora (RÇ, grade (GR, enxada rotativa (RT, herbicida de pós-emergência (HC, herbicida de pré-emergência (HR, capina manual (CM e testemunha sem capina (SC, dispostos em blocos casualizados com três repetições. Amostras de solo, em cada tratamento, foram coletadas a cada dois anos, a partir de 1980, nas camadas de 0-0,15 e 0,15-0,30 m, para avaliação de pH, Al3+, acidez potencial (H + Al e saturação por Al3+ (m. O sistema HR aumentou o teor e a saturação por Al3+ e a acidez potencial e diminuiu o pH, quando comparado com os demais métodos de controle de plantas invasoras, principalmente com a testemunha (SC. O tratamento SC mostrou efeito contrário ao do HR, aumentando os valores de pH e diminuindo o teor de Al3+ e a saturação por Al3+, em ambas as camadas de solo. O RÇ foi o tratamento que mais se aproximou do SC, e os demais tratamentos, no geral, não apresentaram comportamento diferenciado.In perennial agriculture, such as coffee plantation, weeds are controlled by hand, mechanized, and chemical weeding and their combinations. Methods that differ in terms of efficiency and costs have been evaluated; however, the influence of these methods on the soil chemical conditions has

  4. Faixas críticas de teores foliares de macronutrientes em mudas de cafeeiro (Coffea arabica L. produzidas em tubetes Critical ranges of macronutrient content in leaves of coffee seedlings (Coffea arabica L. grown in plastic pots

    Directory of Open Access Journals (Sweden)

    Sergio Moraes Gonçalves

    2009-06-01

    Full Text Available Conduziu-se este trabalho, com o objetivo de determinar os teores foliares de macronutrientes em mudas de cafeeiro produzidas em tubetes. O experimento foi conduzido em viveiro localizado no Setor de Cafeicultura do Departamento de Agricultura da Universidade Federal de Lavras (UFLA, no período de maio de 2003 a janeiro de 2004. Foi utilizado o delineamento de blocos casualizados em esquema fatorial simples 6 x 3 com quatro blocos, sendo seis níveis de adubação do substrato (50, 75, 100, 125, 150 e 200% da dose padrão de Osmocote por m³ de substrato e três estádios de desenvolvimento das plantas: 3, 4 e 5 pares de folhas. Foram avaliadas as seguintes características: altura de planta (cm, diâmetro de caule (mm, área foliar (cm², massa seca de raiz (g, massa seca de caule (g, massa seca das folhas (g, massa seca de parte aérea (g, massa seca total (g e concentrações foliares de: N, P, K, Ca, Mg, S. As faixas críticas de teores obtidas para macronutrientes são as seguintes: nitrogênio (2,26 a 2,62 dag/Kg; fósforo (0,22 a 0,25 dag/Kg; potássio (2,59 a 2,92 dag/Kg; cálcio (0,69 a 0,76 dag/Kg; magnésio (0,11 a 0,12 dag/Kg; enxofre (0,15 a 0,24 dag/Kg. Além disso, constatou-se que o estádio de 4 pares de folhas verdadeiras é o ideal para a coleta de folhas visando à avaliação do estado nutricional das mudas.The aim of this study was to determinate the macronutrient content in leaves of coffee seedlings grown in plastic pots. The experiment was carried out at a greenhouse located in the coffee research area at the Agronomy Department of Lavras Federal University from May 2003 to January 2004. We used a block design in a sample factorial 6 x 3 with four blocks, where the substrate was treated with six levels of fertilization (50, 75, 100, 125, 150, and 200% of standard fertilization with Osmocote for m³ substrate and the evaluations were performed at three stages of development (sampling times: three, four, and five pairs of

  5. Proteolytic activities of bacteria, yeasts and filamentous fungi isolated from coffee fruit (Coffea arabica L. = Atividade proteolítica de bactérias, leveduras e fungos filamentosos presentes em grãos de café (Coffea arabica L.

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    Mirian Pereira Rodarte

    2011-07-01

    Full Text Available One hundred forty-four microorganisms previously isolated from coffee fruit (Coffea arabica were grown on casein agar to evaluate their proteolytic activities. Fifty percent of filamentous fungi, 52.5% of bacteria and 2.6% of yeasts were able to secrete proteases. Positiveisolates were further examined in liquid culture for their protease activities by hydrolysis of casein at different pH values (5.0, 7.0 and 9.0 at 30 oC. Bacillus megaterium, B. subtilis, Enterobacteragglomerans, Kurthia sp, Pseudomonas paucimobilis and Tatumella ptyseos demonstrated the highest proteolytic activities at pH 9.0. One yeast isolate, Citeromyces matritensis, had a proteolytic activityof 2.40 U at pH 5.0. Aspergillus dimorphicus, A. ochraceus, Fusarium moniliforme, F. solani, Penicillium fellutanum and P. waksmanii showed the highest activities. Of the bacterial isolates, the highestenzyme activities were observed in B. subtilis 333 (27.1 U, Tatumella ptyseos (27.0 U and B. megaterium 817 (26.2 U. Of the filamentous fungi, Aspergillus ochraceus (48.7 U, Fusarium moniliforme 221 (37.5 U and F. solani 359 (37.4 U had the highest activities at pH 9.0. Este trabalho teve por objetivos avaliar a capacidade de secreção de proteases extracelulares por 144 microrganismos, previamente isoladosde grãos de café (Coffea arabica durante fermentação por via seca, e determinar a atividade das enzimas produzidas. Os microrganismos foram cultivados em ágar-caseína para avaliação da produção de enzimas proteolíticas. Dos 40 isolados de bactéria presentes na amostra, 52,5% apresentaram resultado positivo para o teste. Considerando os 66 isolados de fungos filamentosos, 50% foram capazes de secretar proteases, enquanto que dos 38 isolados de leveduras, apenas 2,6% conseguiram promover a hidrólise da caseína do meio. Os isolados que apresentaram capacidade de secreção de proteases foram, posteriormente, cultivados em meio líquido para a determinação da atividade

  6. Recuperação do cafeeiro (Coffea arabica L. após recepa, submetido a diferentes lâminas de água e parcelamentos da adubação = Recovery of coffee tree (Coffea arabica L. after pruning under different irrigation depths

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    Kelte Resende Arantes

    2009-04-01

    Full Text Available Visando reduzir o período de recuperação das lavouras após a recepa, neste trabalho objetivou-se avaliar a influência de diferentes lâminas de irrigação e do parcelamento da adubação sobre o crescimento do cafeeiro. Foram utilizadas quatro diferentes lâminas de irrigação (0, 40, 80 e 120% da ECA-Precipitação e três parcelamentos da adubação com N e K (quatro, oito e 12 vezes. O experimento foi conduzido no Sul de Minas Gerais, com o cafeeiro (Coffea arabica cv. Topázio MG-1190, plantado no espaçamento de 1,8 x 0,7 m e recepado 65 meses após o plantio. O delineamentoexperimental utilizado foi em blocos casualizados com parcelas subdivididas. Os parâmetros avaliados foram altura da planta, diâmetro do ramo ortotrópico, número de internódios e diâmetro da copa. O crescimento do cafeeiro foi influenciado pela irrigação, mas não foi influenciado pelo parcelamento da adubação. A lâmina de 120% do saldo do balanço ECA - P proporcionou maior crescimento.In order to reduce the recovery period of the orchard coffeeafter pruning, this work was carried out with the objective of evaluating the influence of different irrigation depths and splitting of N and K with fertirrigation on the growth of coffee plants. The experiment was carried out in southern Minas Gerais State with coffeaarabica cv. Topázio MG-1190 implanted with 1.8 x 0.7 m spacing. Irrigations corresponded to 0, 40, 80 and 120% of the evaporation from a Class A pan – precipitation (ECA-P and N and K split in 4, 8 and 12 applications. A randomized block design with four replicationswas used. Plant height, diameter of orthotropic branches, number of internodes and crown diameter were evaluated. The fertilizer splitting was found not to provide any increase in growth of the coffee plant. The depth of 120%*ECA-P provided the greatest growth ofcoffee plants when compared to others.

  7. Produtividade de cultivares de cafeeiros (Coffea arabica L. submetidas a diferentes épocas de parcelamento da adubação Yield of coffee cultivars (Coffea arabica L. under different split-fertilization times

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    Gabriel Ferreira Bartholo

    2003-08-01

    Full Text Available Os experimentos foram instalados na Fazenda Experimental da Epamig em São Sebastião do Paraíso, com o objetivo de estudar o comportamento das cultivares Mundo Novo-IAC 379/19; Icatu Precoce-IAC 3282; Icatu Amarelo-IAC 2944 e Rubi-MG 1192, em relação a épocas de parcelamento de adubação. As respostas das cultivares foram medidas sobre a produção em função das épocas de parcelamento da adubação, demonstrando que a característica estudada foi influenciada pelas combinações das épocas em que foram submetidas. A cultivar Mundo Novo-IAC 379/19 tolerou intervalos maiores entre as adubações no período de outubro a março, e a cultivar Icatu Amarelo-IAC 2944 respondeu de modo significativo a quatro parcelamentos consecutivos com intervalo de 30-40 dias entre as aplicações. Para a cultivar Icatu Precoce-IAC 3282, existem opções de estabelecimento de épocas adequadas, dependendo do início das chuvas. A cultivar Rubi-MG 1192 não respondeu às épocas de parcelamento da adubação.ABSTRACT - The work was conducted at EPAMIG's Experimental Station in São Sebastião do Paraíso, MG, to study the performance of Mundo Novo-IAC 379/19; Icatu Precoce-IAC 3282; Icatu Amarelo-IAC 2944 and Rubi- MG 1192 coffee cultivars, in relation to time and parceling out of fertilization. Cultivar responses were evaluated by yield measurements, with the results showing that this parameter was affected by time in which fertilization was made. Mundo Novo-IAC 379/19 cultivar tolerated broader intervals between the fertilizations from October to March. Icatu Amarelo-IAC 2944 response was significant to four consecutive parceling, with intervals from 30 to 40 days between applications. Icatu Precoce-IAC 3282 cultivar presented other more appropriate time options depending on the start of rainy season. Rubi-MG 1192 cultivar was not responsive to split-fertilization.

  8. Fatores antinutricionais da casca e da polpa desidratada de café (Coffea arabica L. armazenadas em diferentes períodos Antinutritional factors of the hull and dehydrated pulp of coffee (Coffea arabica L. stored in different periods

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    Adauto Ferreira Barcelos

    2001-07-01

    Full Text Available Avaliaram-se os teores de cafeína, taninos, lignina e sílica, na casca e polpa de café das cultivares Catuaí, Rubi e Mundo Novo. A polpa foi obtida pela despolpa úmida em despolpador mecânico e, em seguida, seca ao sol até 13% de umidade. Os materiais foram armazenados em sacos de ráfia, em ambiente coberto, ventilado e seco, com amostragem em triplicata a cada 90 dias. A regressão mostrou aumento quadrático de 11,7% no teor de cafeína ao longo de 360 dias de armazenamento. O teor de taninos reduziu-se linearmente ao longo do armazenamento. Os valores de taninos foram de 1,70% comparado a 2,77% nos materiais sem armazenamento, redução de aproximadamente 38,6% no período de um ano. Os teores de lignina reduziram linearmente em 2,6% para a porcentagem de lignina na MS (11,7 para 11,4% e 5,8% na porcentagem de lignina da FDN (10,4 para 9,8%, nos materiais sem armazenagem comparados a doze meses de armazenamento. Houve diferença significativa entre casca e polpa para a variável sílica. Maior valor de sílica na casca comparado à polpa pode ser decorrente da presença do pergaminho, uma vez que a polpa não o possui. A armazenagem da casca e polpa por um período de doze meses melhora as qualidades destes materiais, uma vez que reduziu os teores de taninos e lignina. Os teores de cafeína encontrados são limitantes na utilização de grandes quantidades desses materiais para ruminantes.It was evaluated the caffeine contents of caffeine, tannins, lignin and silica in the hull and pulp of coffee of cultivars Catuaí, Rubi, Mundo Novo. Pulp was obtained by moist pulping in a mechanical pulper and dried in the sun adjusted to 13% moisture. Materials were stored in raffia bag in environment free of moisture and ventilated with samplings every 90 days. The quadratic effect shown increased caffeine content along 360 day storage, this increase was of 11,7% along 12 months. Tannin content was reduced linearly along the storage. The values

  9. Parâmetro bromatológicos de grãos crus e torrados de cultivares de café(Coffea arabica L. Bromatological parameters in raw and toasted grains of cultivars of coffee (Coffea arabica L.

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    Luciana Maria Vieira Lopes Mendonça

    2005-06-01

    de São Sebastião do Paraíso da Empresa de Pesquisa Agropecuária de Minas Gerais. The fruits were dry with all the parts creating the known coffee as natural. The toasted grains were obtained by clear roasted, determined visually. The analysis of the results allowed to observe differences in the levels of soluble solids and pH for all the variable studied, in raw and toasted grains. Both cultivars Icatu Amarelo (H 2944 and Acaiá Cerrado presented larger values of the pH in the raw grains and the smallest ones in the toasted grains. The levels of soluble solids were larger in the toasted grains of the cultivars Mundo Novo and Rubi, the smallest values observed for the raw grain were in cultivars Topázio and Rubi. The total acidity showed differences only in toasted grains. There was an increase in the total acidity with the roasting, and reduction in pH values and soluble solids, which showed variation among all cultivars. It was observed that cultivars presented differences in chemical composition, and variations different from this composition with the toasted process.

  10. Effects of gamma radiation of Cobalt-60 on arabica and conillon seeds coffea: physic-chemistry evaluation

    International Nuclear Information System (INIS)

    Silva, Marcus Henriques da

    2012-01-01

    Brazil is the largest producer and exporter of coffee in the world. The coffee bean is one of the main products of the Brazilian trade balance. Two species of coffee are the most economically important: the Coffea arabica L. and Coffea canephora Pierre is the largest representative of the Coffea canephora Pierre is the coffea conillon. Food irradiation is an area of research that aims to increase the shelf life of foods and controlling pests. This study aimed to verify the physicochemical variables of Arabica coffee and conillon were affected when exposed to doses of gamma radiation from cobalt-60. The samples were provided by Polo in Coffee Quality Technology, Federal University of Lavras - UFLA. The coffee samples were subjected to irradiation doses: 0 (control), 5 kGy and 10 kGy, a multipurpose irradiator of IPEN - Research Institute of Nuclear Energy and the University of São Paulo, at a rate of 7.5 kGy / hour. For irradiation the samples were vacuum-packed in appropriate packaging aluminised. After the process of irradiation the samples were stored at a temperature of 15 ± 1 deg C and relative humidity of 17 ± 1%. The following analyzes were performed: levels of total sugars, glucose, sucrose, caffeine, humidity, pH, total acidity, electrical conductivity and fibers. Analyses were performed 1, 30, 60 and 90 days after irradiation, and the results were submitted to analysis of variance and means were compared by Tukey test at 5%. It was observed that the analysis results of the samples irradiated with 5 kGy and 10 kGy showed values similar to the control. It was concluded that irradiation did not induce deleterious effects on arabica coffee seeds and conillon irradiated with 5 kGy and 10 kGy to 90 days after irradiation. (author)

  11. Anatomical and chemical properties and density of Coffea arabica L. wood

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    Marisa Aparecida Pereira

    2014-09-01

    Full Text Available The state of Minas Gerais is the largest producer of coffee in Brazil and the amount of residue in crops seems adequate to support production of solid wood products of Coffea arabica L., which is currently used for energy purposes or remains in the area. This activity adds insignificant value the coffee products and release CO2, which has harmful effects to the environment. This study was conducted with the aim of characterizing technologically Coffea arabica L. wood to enhance its use in furniture, to characterize its anatomical, chemical and wood basic density. The density showed an average of 0.608g.cm-3. The anatomical analysis showed distinct growth layers, semiporosos vessels with simple perforation plates. The axial parenchyma is apotracheal and diffuse in the aggregate with heterogeneous rays, not laminated and fiber libriformes not septate with bordered pits distinct. The chemical content of extract in hot and cold water were respectively 6.1% and 9.6%. The ash content was found to be 0.68%. Data were comparable to those of mahogany (Swietenia macrophylla and Piptadenia peregrina Benth, (angico-vermelho used for the production of furniture.

  12. A Study of Allelopathy of Some Shade Trees to Coffea arabicaL. Seedlings

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    Adi Prawoto

    2006-05-01

    recommended as shade trees or intercrops with Arabica coffee and for D. zibethinusits cropping pattern must be arranged so the mineral competition could be maintained minimum. Key words: Allelopathy, Coffea arabica, Macadamia integrifolia, Cinnamomum burmani, Cassia siamea, Cassia spectabilis, mineral.

  13. Genetic diversity among 16 genotypes of Coffea arabica in the Brazilian cerrado.

    Science.gov (United States)

    Machado, C M S; Pimentel, N S; Golynsk, A; Ferreira, A; Vieira, H D; Partelli, F L

    2017-09-21

    For the selection of coffee plants that have favorable characteristics, it is necessary to evaluate variables related to production. Knowledge of the genetic divergence of arabica coffee is of extreme importance, as this knowledge can be associated with plant breeding programs in order to combine genetic divergence with good productive performance. The objective of this study was to evaluate the genetic divergence among 16 genotypes of Coffea arabica with the purpose of identifying the most dissimilar genotypes for the establishment of breeding programs and adaptation to the Brazilian cerrado. The genetic divergence was evaluated using multivariate procedures, the analysis of the average grouping unweighted pair group method with arithmetic mean (UPGMA) and main components in 2013 and 2014. Eight characters were evaluated in an experiment conducted in Morrinhos, Goiás. The presence of genetic divergence among the 16 C. arabica genotypes under cerrado conditions was recorded. The formation of UPGMA groups for the evaluated characteristics was pertinent due to the number of genotypes. The first three major components accounted for 81.77% of the total variance. The genotype H-419-3-4-4-13(C-241) of low size was the most divergent, followed by Catucaí 2 SL and Catiguá MG2, according to the main components.

  14. Rust and Thinning Management Effect on Cup Quality and Plant Performance for Two Cultivars of Coffea arabica L.

    Science.gov (United States)

    Echeverria-Beirute, Fabian; Murray, Seth C; Klein, Patricia; Kerth, Chris; Miller, Rhonda; Bertrand, Benoit

    2018-05-30

    Beverage quality is a complex attribute of coffee ( Coffea arabica L.). Genotype (G), environment (E), management (M), postharvest processing, and roasting are all involved. However, little is known about how G × M interactions influence beverage quality. We investigated how yield and coffee leaf rust (CLR) disease (caused by Hemileia vastatrix Berk. et Br.) management affect cup quality and plant performance, in two coffee cultivars. Sensory and chemical analyses revealed that 10 of 70 attributes and 18 of 154 chemical volatile compounds were significantly affected by G and M. Remarkably, acetaminophen was found for the first time in roasted coffee and in higher concentrations under more stressful conditions. A principal component analysis described 87% of the variation in quality and plant overall performance. This study is a first step in understanding the complexity of the physiological, metabolic, and molecular changes in coffee production, which will be useful for the improvement of coffee cultivars.

  15. Phytochemical Composition, Antioxidant Activity, and the Effect of the Aqueous Extract of Coffee (Coffea arabica L.) Bean Residual Press Cake on the Skin Wound Healing.

    Science.gov (United States)

    Affonso, Regina Celis Lopes; Voytena, Ana Paula Lorenzen; Fanan, Simone; Pitz, Heloísa; Coelho, Daniela Sousa; Horstmann, Ana Luiza; Pereira, Aline; Uarrota, Virgílio Gavicho; Hillmann, Maria Clara; Varela, Lucas Andre Calbusch; Ribeiro-do-Valle, Rosa Maria; Maraschin, Marcelo

    2016-01-01

    The world coffee consumption has been growing for its appreciated taste and its beneficial effects on health. The residual biomass of coffee, originated in the food industry after oil extraction from coffee beans, called coffee beans residual press cake, has attracted interest as a source of compounds with antioxidant activity. This study investigated the chemical composition of aqueous extracts of coffee beans residual press cake (AE), their antioxidant activity, and the effect of topical application on the skin wound healing, in animal model, of hydrogels containing the AE, chlorogenic acid (CGA), allantoin (positive control), and carbopol (negative control). The treatments' performance was compared by measuring the reduction of the wound area, with superior result ( p coffee AE (78.20%) with respect to roasted coffee AE (53.71%), allantoin (70.83%), and carbopol (23.56%). CGA hydrogels reduced significantly the wound area size on the inflammatory phase, which may be associated with the well known antioxidant and anti-inflammatory actions of that compound. The topic use of the coffee AE studied improved the skin wound healing and points to an interesting biotechnological application of the coffee bean residual press cake.

  16. Phytochemical Composition, Antioxidant Activity, and the Effect of the Aqueous Extract of Coffee (Coffea arabica L. Bean Residual Press Cake on the Skin Wound Healing

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    Regina Celis Lopes Affonso

    2016-01-01

    Full Text Available The world coffee consumption has been growing for its appreciated taste and its beneficial effects on health. The residual biomass of coffee, originated in the food industry after oil extraction from coffee beans, called coffee beans residual press cake, has attracted interest as a source of compounds with antioxidant activity. This study investigated the chemical composition of aqueous extracts of coffee beans residual press cake (AE, their antioxidant activity, and the effect of topical application on the skin wound healing, in animal model, of hydrogels containing the AE, chlorogenic acid (CGA, allantoin (positive control, and carbopol (negative control. The treatments’ performance was compared by measuring the reduction of the wound area, with superior result (p<0.05 for the green coffee AE (78.20% with respect to roasted coffee AE (53.71%, allantoin (70.83%, and carbopol (23.56%. CGA hydrogels reduced significantly the wound area size on the inflammatory phase, which may be associated with the well known antioxidant and anti-inflammatory actions of that compound. The topic use of the coffee AE studied improved the skin wound healing and points to an interesting biotechnological application of the coffee bean residual press cake.

  17. Perdas causadas por Coccus viridis (Hemiptera: Coccidae em mudas de Coffea arabica L.

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    Flávio Lemes Fernandes

    2009-08-01

    Full Text Available Coccus viridis (Green danifica plantas jovens e adultas de Coffea arabica Linnaeu. No entanto, nada se sabe sobre a magnitude dos danos causados por esta praga. Assim, este trabalho teve por objetivo estudar as relações entre o ataque de C. viridis e as perdas causadas por este inseto a C. arabica. Este trabalho foi conduzido em casa de vegetação na Universidade Federal de Viçosa. Foram utilizadas sementes da linhagem IAC 15 da variedade “Catuaí vermelho” de café (C. arabica. Para a confecção dos tratamentos esta praga foi criada em casa de vegetação separada do experimento. Os tratamentos foram: plantas infestadas e não infestadas por adultos e ninfas da cochonilha verde. As plantas foram nutridas com solução nutritiva. Durante 110 dias foram avaliados: números de adultos e de ninfas de primeiro, segundo e terceiro ínstares, área foliar, diâmetro do caule, altura das plantas em todas repetições. No final do experimento avaliou-se o peso das raízes, caule, folhas e total. Os pesos das raízes, matéria seca total, área foliar e diâmetro do caule de plantas não atacadas por C. viridis superaram em 1,31; 1,41; 1,50 e 8,93 vezes, respectivamente o peso de plantas atacadas. As variáveis selecionadas foram: diâmetro do caule (cm, área foliar (cm², peso de raízes (g, ninfas, adultos e total das cochonilhas. Concluindo que a planta de C. arabica é afetada de forma diferente entre seus órgãos e que a ninfa de terceiro ínstar e adultos são as fases que mais causam danos a C. arabica.Losses Caused by Coccus viridis (Green (Hemiptera: Coccidae on Seedlings of Coffea arabica L.Abstract. Coccus viridis (Green cause losses on seedling and old plants of Coffea arabica (Green. However, nothing is known about of the damages caused by this pest. Thus, the objective of this work was to evaluate the relations between atack of C. viridis and the losses caused by this insect. on C. arabica. This work was conduced in greenhouse at

  18. Avaliação de produtividade de progênies de cafeeiro em dois sistemas de plantio Yield evaluation of coffee plant (Coffea arabica L. progenies in two tillage systems

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    Gladyston Rodrigues Carvalho

    2006-10-01

    Full Text Available Objetivou-se com o presente trabalho analisar a influência do sistema de plantio (adensado e convencional e a produtividade de cafeeiros (Coffea arabica L., resultantes do cruzamento de Catuaí Vermelho IAC 99 com Mundo Novo IAC 379-19. Foram utilizadas 29 progênies, na 4ª geração por autofecundação do 2º retrocruzamento de Catuaí Vermelho com Mundo Novo desenvolvidas pelo programa de Melhoramento Genético do Cafeeiro em Minas Gerais, coordenado pela EPAMIG. Utilizou-se como testemunha 13 cultivares, Topázio MG-1189 e 1189 SL, Catuaí Vermelho IAC-15, IAC-99 e IAC-144, Catuaí Amarelo MG-17, Rubi MG-1192 e 1192SL, Acaiá Cerrado MG-1474 e 1474 SL, Mundo Novo IAC379-19, IAC-376-4 e 376-4 SL. O experimento foi instalado na Fazenda Experimental da EPAMIG, em São Sebastião do Paraíso, MG em janeiro de 1996 no espaçamento de 3,50 x 1,00 m (sistema convencional e 1,80 x 0,60 m (sistema adensado. O delineamento experimental foi em blocos casualizados, sendo os tratamentos compostos por 42 progênies/cultivares com três repetições e seis plantas por parcela com a análise de variância, em parcelas subdivididas, onde cada biênio (agrupamento de colheita foi considerado como uma subparcela. Avaliou-se a produção de grãos em sacas de 60 kg de café beneficiado/ha. Verificou-se nos resultados que a produtividade inicial no sistema adensado foi maior que no sistema convencional, porém com base no desenvolvimento das plantas essa diferença tendeu a diminuir. Para ambos os sistemas de plantio, pode-se utilizar qualquer uma das cultivares uma vez que as mesmas comportaram-se de forma semelhante. As cultivares utilizadas como testemunha que apresentaram as maiores produtividades foram Topázio MG-1189 e 1189 SL, Rubi MG-1192 e 1192SL, Catuaí Vermelho IAC 15 e IAC 144 e Mundo Novo IAC-376-4 e 376-4SL.The present work was carried out in order to analyze the planting system (high and low plant stand influence on coffee trees (Coffea

  19. Efficiency of removal of bod5 and ss in sedimentation tanks and filters in wastewater treatment systems for coffee bean (Coffea arabica processing

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    Nelson Gutiérrez Guzmán

    2014-12-01

    Full Text Available n order to evaluate the current operating conditions of wastewater treatment systems of small scale coffee growers in the south of Huila a lab-scale prototype (S 1:25 was constructed. It was composed of both a sediment tank and a filter fit in series, simulating similar operating conditions used by coffee producers. Removal of biological oxygen demand (BOD5 and suspended solids (SS was performed in wastewater from coffee bean processing. A 23 factorial experimental design for the evaluation of the type of sedimentation tank, type of filter and hydraulic retention time (HRT in the sedimentation tank was employed. The results showed high removal efficiencies of suspended solid concentrations (more than 95%, and low removal efficiencies in BOD5 (about 20%. The combination of tank type 1 (square with a lower area, filter type 1 (upflow anaerobic filter – UAF and HRT of 30 hours had the highest removal efficiency.

  20. Genetic molecular analysis of Coffea arabica (Rubiaceae hybrids using SRAP markers

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    Manoj Kumar Mishra

    2011-06-01

    Full Text Available In Coffea arabica (arabica coffee, the phenotypic as well as genetic variability has been found low because of the narrow genetic basis and self fertile nature of the species. Because of high similarity in phenotypic appearance among the majority of arabica collections, selection of parental lines for inter-varietals hybridization and identification of resultant hybrids at an early stage of plant growth is difficult. DNA markers are known to be reliable in identifying closely related cultivars and hybrids. Sequence Related Amplified Polymorphism (SRAP is a new molecular marker technology developed based on PCR. In this paper, sixty arabica-hybrid progenies belonging to six crosses were analyzed using 31 highly polymorphic SRAP markers. The analysis revealed seven types of SRAP marker profiles which are useful in discriminating the parents and hybrids. The number of bands amplified per primer pair ranges from 6.13 to 8.58 with average number of seven bands. Among six hybrid combinations, percentage of bands shared between hybrids and their parents ranged from 66.29% to 85.71% with polymorphic bands varied from 27.64% to 60.0%. Percentage of hybrid specific fragments obtained in various hybrid combinations ranged from 0.71% to 10.86% and ascribed to the consequence of meiotic recombination. Based on the similarity index calculation, it was observed that F1 hybrids share maximum number of bands with the female parent compared to male parent. The results obtained in the present study revealed the effectiveness of SRAP technique in cultivar identification and hybrid analysis in this coffee species. Rev. Biol. Trop. 59 (2: 607-617. Epub 2011 June 01.

  1. O dimorfismo dos ramos em Coffea arabica L.

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    A. Carvalho

    1950-06-01

    branches was found to be permanent and irreversible. The same irreversible condition was observed to be true of rooted stem cuttings of plagiotropic branches. A special study was made of Coffea arabica var. erecta, a dominant mutant, which has upright growing lateral branches. Experiments revealed that even in this coffee genotype dimorphism exists, as grafts obtained from lateral branches only formed low shrubs, in spite of the fact their branches grew upright. Even with this specific genotype it was found necessary to use the tips of orthotropic branches in order to obtain normal plant development. Several hypothesis are presented which might explain this phenomenon. Attention is called to the fact that the buds which exist close together in the leaf axils of the main stem have already differentiated into either the orthotropic or plagiotropic form and that their subsequent growth will be governed by this prior differentation.

  2. Application of thermography for monitoring stomatal conductance of Coffea arabica under different shading systems.

    Science.gov (United States)

    Craparo, A C W; Steppe, K; Van Asten, P J A; Läderach, P; Jassogne, L T P; Grab, S W

    2017-12-31

    Stomatal regulation is a key process in the physiology of Coffea arabica (C. arabica). Intrinsically linked to photosynthesis and water relations, it provides insights into the plant's adaptive capacity, survival and growth. The ability to rapidly quantify this parameter for C. arabica under different agroecological systems would be an indispensable tool. Using a Flir E6 MIR Camera, an index that is equivalent to stomatal conductance (I g ) was compared with stomatal conductance measurements (g s ) in a mature coffee plantation. In order to account for varying meteorological conditions between days, the methods were also compared under stable meteorological conditions in a laboratory and I g was also converted to absolute stomatal conductance values (g 1 ). In contrast to typical plant-thermography methods which measure indices once per day over an extended time period, we used high resolution hourly measurements over daily time series with 9 sun and 9 shade replicates. Eight daily time series showed a strong correlation between methods, while the remaining 10 were not significant. Including several other meteorological parameters in the calculation of g 1 did not contribute to any stronger correlation between methods. Total pooled data (combined daily series) resulted in a correlation of ρ=0.66 (P≤2.2e-16), indicating that our approach is particularly useful for situations where absolute values of stomatal conductance are not required, such as for comparative purposes, screening or trend analysis. We use the findings to advance the protocol for a more accurate methodology which may assist in quantifying advantageous microenvironment designs for coffee, considering the current and future climates of coffee growing regions. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  3. Evaluation of a treatment system wastewater prebeneficiado of coffee (Coffea arabica implemented in the community Carmen Pampa province of Nor Yungas of La Paz Department

    Directory of Open Access Journals (Sweden)

    Álvarez Javier

    2011-08-01

    Full Text Available This research was conducted in prebeneficiadora coffee plant of UAC-CP, located in the community of Carmen Pampa, Coroico Municipality, Nor Yungas of La Paz department. The objective was to determine the efficiency of wastewater treatment prebeneficiado coffee because it is not known to be effective in reducing the level of contamination of treated wastewater, and according to the results to correct and / or validate directly model system in place. For their study was established seven sampling points within the system of treatment and four replications were made at different times and with different amounts of coffee cherry. We evaluated the parameters of temperature, pH, settleable solids, total suspended solids, fixed and volatile, biochemical oxygen demand, chemical oxygen demand and dissolved oxygen. The temperature of wastewater entering the system has recorded 16.5 o C, increasing during the treatment process at 17.5 °C and record the output is 16.5 ºC. The pH increased from 4.51 to 6.04 which occurred at the last two gaps oxygenation and not in the neutralization tank. Settleable solids (SS increased from 0.1 mg / L to 0.15 mg / L, which was recorded at the lakes of oxygen, is attributed to the drag of fine gravel. Total suspended solids (TSS have been reduced from 1927.5 mg / L to 299 mg / L gradually throughout the treatment process. Fixed suspended solids (SSF were reduced from 137.5 mg / L to 58.5 mg / L. Volatile suspended solids (VSS were reduced from 1790 mg / L to 240.5 mg / L. Biochemical oxygen demand (BOD was reduced from 6102.5 mg O2 / L to 1245 mg O2 / L. The chemical oxygen demand (COD was reduced from 9800 mg O2 / L to 1658.75 mg O2 / L. Dissolved oxygen (DO increased from 0 to 2.4 mg O2 / L, in the last two gaps in oxygenation, due to the reduction of solids and chemical oxygen demand and biochemical oxygen. Of the nine parameters measured, six meet the maximum permissible values for download in source receptor (stream

  4. Water use efficiency by coffee arabica after glyphosate application

    Directory of Open Access Journals (Sweden)

    Felipe Paolinelli de Carvalho

    2014-07-01

    Full Text Available Many coffee growers apply glyphosate in directed applications, but some phytotoxicity has been noted. It is believed some herbicides can exert a direct or indirect negative effect on photosynthesis by reducing the metabolic rate in a way that can affect the water use efficiency. The objective of this study was to investigate the variables related to water use among coffee cultivars subjected to the application of glyphosate and the effects of each dose. The experiment was conducted in a greenhouse using three varieties of coffee (Coffea arabica, Acaiá (MG-6851, Catucaí Amarelo (2SL and Topázio (MG-1190, and three doses of glyphosate (0.0, 115.2 and 460.8 g acid equivalent ha-1, in a factorial 3 x 3 design. At 15 days after application, a reduction in stomatal conductance was observed, and smaller transpiration rate and water use efficiency were found in the fourth leaf at 15 days after application. There was a decrease in the transpiration rate at 45 DAA, with the Acaiá cultivar showing reductions with 115.2 g ha-1. There was transitory reduction in water use efficiency with glyphosate application, but can affect the growth and production. The Acaiá cultivar showed the highest tolerance to glyphosate because the water use efficiency after herbicide application.

  5. Effects of gamma radiation of Cobalt-60 on arabica and conillon seeds coffea: physic-chemistry evaluation; Efeitos da radiacao gama do Cobalto-60 em sementes de cafe arabica e conillon: avaliacao fisico-quimica

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Marcus Henriques da

    2012-07-01

    Brazil is the largest producer and exporter of coffee in the world. The coffee bean is one of the main products of the Brazilian trade balance. Two species of coffee are the most economically important: the Coffea arabica L. and Coffea canephora Pierre is the largest representative of the Coffea canephora Pierre is the coffea conillon. Food irradiation is an area of research that aims to increase the shelf life of foods and controlling pests. This study aimed to verify the physicochemical variables of Arabica coffee and conillon were affected when exposed to doses of gamma radiation from cobalt-60. The samples were provided by Polo in Coffee Quality Technology, Federal University of Lavras - UFLA. The coffee samples were subjected to irradiation doses: 0 (control), 5 kGy and 10 kGy, a multipurpose irradiator of IPEN - Research Institute of Nuclear Energy and the University of São Paulo, at a rate of 7.5 kGy / hour. For irradiation the samples were vacuum-packed in appropriate packaging aluminised. After the process of irradiation the samples were stored at a temperature of 15 ± 1 deg C and relative humidity of 17 ± 1%. The following analyzes were performed: levels of total sugars, glucose, sucrose, caffeine, humidity, pH, total acidity, electrical conductivity and fibers. Analyses were performed 1, 30, 60 and 90 days after irradiation, and the results were submitted to analysis of variance and means were compared by Tukey test at 5%. It was observed that the analysis results of the samples irradiated with 5 kGy and 10 kGy showed values similar to the control. It was concluded that irradiation did not induce deleterious effects on arabica coffee seeds and conillon irradiated with 5 kGy and 10 kGy to 90 days after irradiation. (author)

  6. Homostachydrine (pipecolic acid betaine) as authentication marker of roasted blends of Coffea arabica and Coffea canephora (Robusta) beans.

    Science.gov (United States)

    Servillo, Luigi; Giovane, Alfonso; Casale, Rosario; Cautela, Domenico; D'Onofrio, Nunzia; Balestrieri, Maria Luisa; Castaldo, Domenico

    2016-08-15

    The occurrence of pipecolic acid betaine (homostachydrine) and its biosynthetic precursor N-methylpipecolic acid was detected for the first time in green coffee beans of Robusta and Arabica species. The analyses were conducted by HPLC-ESI tandem mass spectrometry and the metabolites identified by product ion spectra and comparison with authentic standards. N-methylpipecolic acid was found at similar levels in green coffee beans of Robusta and Arabica, whereas a noticeable difference of homostachydrine content was observed between the two green coffee bean species. Interestingly, homostachydrine content was found to be unaffected by coffee bean roasting treatment because of a noticeable heat stability, a feature that makes this compound a candidate marker to determine the content of Robusta and Arabica species in roasted coffee blends. To this end, a number of certified pure Arabica and Robusta green beans were analyzed for their homostachydrine content. Results showed that homostachydrine content was 1.5±0.5mg/kg in Arabica beans and 31.0±10.0mg/kg in Robusta beans. Finally, to further support the suitability of homostachydrine as quality marker of roasted blends of Arabica and Robusta coffee beans, commercial samples of roasted ground coffee blends were analyzed and the correspondence between the derived percentages of Arabica and Robusta beans with those declared on packages by manufacturers was verified. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Effect of the inoculation density in Coffea arabica L. cv. `Caturra rojo' somatic embryos germination in RITA® Temporary Immersion System

    Directory of Open Access Journals (Sweden)

    Raúl Barbon

    2014-04-01

    Full Text Available The development of somatic embryogenesis of coffee (Coffea spp. in liquid culture medium is a viable alternative for the propagation of these species. The use of liquid culture medium and temporary immersion systems could increase the germination of somatic embryos and improve the quality of plants. The objective of this work was to determine the effect of inoculation density on germination of somatic embryos of Coffea arabica L. cv. `Caturra rojo' in temporary immersion systems RITA®. It were used as inoculum densities 40, 50, 60, 70 and 80 somatic embryos per RITA®. After 90 days of culture the number of somatic embryos germinated, hyperhydricity symptoms, number of true leaves, length and root development was quantified. With inoculum density of 70 somatic embryos per RITA®, it was obtained a highest germination percentage (60% with good leaf development and length of the plants. Key words: hyperhydricity, liquid culture medium, partial germination, total germination, somatic embryogenesis

  8. Covering the different steps of the coffee processing: Can headspace VOC emissions be exploited to successfully distinguish between Arabica and Robusta?

    Science.gov (United States)

    Colzi, Ilaria; Taiti, Cosimo; Marone, Elettra; Magnelli, Susanna; Gonnelli, Cristina; Mancuso, Stefano

    2017-12-15

    This work was performed to evaluate the possible application of PTR-ToF-MS technique in distinguishing between Coffea arabica (Arabica) and Coffea canephora var. robusta (Robusta) commercial stocks in each step of the processing chain (green beans, roasted beans, ground coffee, brews). volatile organic compounds (VOC) spectra from coffee samples of 7 Arabica and 6 Robusta commercial stocks were recorded and submitted to multivariate statistical analysis. Results clearly showed that, in each stage of the coffee processing, the volatile composition of coffee is highly influenced by the species. Actually, with the exception of green beans, PTR-ToF-MS technique was able to correctly recognize Arabica and Robusta samples. Particularly, among 134 tentatively identified VOCs, some masses (16 for roasted coffee, 12 for ground coffee and 12 for brewed coffee) were found to significantly discriminate the two species. Therefore, headspace VOC analyses was showed to represent a valuable tool to distinguish between Arabica and Robusta. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Sensory Description of Cultivars (Coffea Arabica L. Resistant to Rust and Its Correlation with Caffeine, Trigonelline, and Chlorogenic Acid Compounds

    Directory of Open Access Journals (Sweden)

    Larissa de Oliveira Fassio

    2016-01-01

    Full Text Available Considering the importance of the chemical compounds in Arabica coffee beans in the definition of the drink sensory quality and authentication of coffee regions, the aim of this study was to evaluate, from principal component analysis—PCA—if there is a relation between the caffeine, trigonelline, and chlorogenic acid (5-CQA content and the sensory attributes of the drink, and in this context, enabling the differentiation of cultivars in two coffee-producing regions of Brazil. We evaluated seven rust-resistant Coffea arabica cultivars, and two rust-susceptible cultivars in two cultivation environments: Lavras, in the southern region of Minas Gerais state, and Patrocinio in the Cerrado region of Minas Gerais. The flavor and acidity were determinant for differentiation of the cultivars and their interaction with the evaluated environments. Cultivars Araponga MG1, Catigua MG2, and Catigua MG1 are the most suitable for the production of specialty coffee in the state of Minas Gerais. A poor correlation was found between caffeine, trigonelline, 5-CQA contents, and fragrance, flavor, acidity, body, and final score attributes. However, these compounds enabled the differentiation of the environments. The PCA indicated superiority in the sensory quality of cultivars resistant to rust, compared to the control, Bourbon Amarelo, and Topázio MG1190.

  10. Development of microsatellite markers for identifying Brazilian coffee arabica varieties

    NARCIS (Netherlands)

    Vieira, E.S.N.; Pinho, Von E.V.R.; Carvalho, M.G.G.; Esselink, G.; Vosman, B.

    2010-01-01

    Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of

  11. Identification and chromosomal distribution of copia-like retrotransposon sequences in the coffee (Coffea L. genome

    Directory of Open Access Journals (Sweden)

    Juan-Carlos Herrera

    2013-12-01

    Full Text Available The presence of copia-like transposable elements in seven coffee (Coffea sp. species, including the cultivated Coffea arabica, was investigated. The highly conserved domains of the reverse transcriptase (RT present in the copia retrotransposons were amplified by PCR using degenerated primers. Fragments of roughly 300 bp were obtained and the nucleotide sequence was determined for 36 clones, 19 of which showed good quality. The deduced amino acid sequences were compared by multiple alignment analysis. The data suggested two distinct coffee RT groups, designated as CRTG1 and CRTG2. The sequence identities among the groups ranged from 52 to 60% for CRTG1 and 74 to 85% for CRTG2. The multiple alignment analysis revealed that some of the clones in CRTG1 were closely related to the representative elements present in other plant species such as Brassica napus, Populus ciliata and Picea abis. Furthermore, the chromosomal localization of the RT domains in C. arabica and their putative ancestors was investigated by fluorescence in situ hybridization (FISH analysis. FISH signals were observed throughout the chromosomes following a similar dispersed pattern with some localized regions exhibiting higher concentrations of those elements, providing new evidence of their relative conservation and stability in the coffee genome

  12. Allelopathic effects of two cover crops Commelina diffusa Burm. F. and Tradescantia zebrina Shunltz on Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    Georgina Berroa Navarro

    2016-03-01

    Full Text Available Allelopathic effect of the cover crops Tradescantia zebrina Shunltz (cucaracha and Commelina diffusa Burm. F. (canutillo were evaluated on Coffea arabica Lin. seeds Caturra Rojo variety. Germination tests were carried out “in vitro” and it was evaluated the root longitude, percentage of total germination and period of germination, as well as the height of the plant and the emergency percentage for the incorporation tests to the soil. It was also carried out, to both over crops, the preliminary chemical qualitative characterization. The results showed that the extracts of T. zebrina and of C. diffusa stimulated the “in vitro” germination and growth of C. arabica at different concentration levels. The incorporation to the soil of the extracts of C. diffusa stimulated the development of the plants of C. arabica, in a significant way, that supposes a considerable advantage in that concerns to the employment of these cover crops, when not implying noxious effects beside all the benefits implied when using cover crops. These last ones go from the protection and improvement of the properties of the soil, to the control of the spontaneous flora in the coffee agroecosystems.

  13. Identification and Analysis of Jasmonate Pathway Genes in Coffea canephora (Robusta Coffee) by In Silico Approach.

    Science.gov (United States)

    Bharathi, Kosaraju; Sreenath, H L

    2017-07-01

    Coffea canephora is the commonly cultivated coffee species in the world along with Coffea arabica . Different pests and pathogens affect the production and quality of the coffee. Jasmonic acid (JA) is a plant hormone which plays an important role in plants growth, development, and defense mechanisms, particularly against insect pests. The key enzymes involved in the production of JA are lipoxygenase, allene oxide synthase, allene oxide cyclase, and 12-oxo-phytodienoic reductase. There is no report on the genes involved in JA pathway in coffee plants. We made an attempt to identify and analyze the genes coding for these enzymes in C. canephora . First, protein sequences of jasmonate pathway genes from model plant Arabidopsis thaliana were identified in the National Center for Biotechnology Information (NCBI) database. These protein sequences were used to search the web-based database Coffee Genome Hub to identify homologous protein sequences in C. canephora genome using Basic Local Alignment Search Tool (BLAST). Homologous protein sequences for key genes were identified in the C. canephora genome database. Protein sequences of the top matches were in turn used to search in NCBI database using BLAST tool to confirm the identity of the selected proteins and to identify closely related genes in species. The protein sequences from C. canephora database and the top matches in NCBI were aligned, and phylogenetic trees were constructed using MEGA6 software and identified the genetic distance of the respective genes. The study identified the four key genes of JA pathway in C. canephora , confirming the conserved nature of the pathway in coffee. The study expected to be useful to further explore the defense mechanisms of coffee plants. JA is a plant hormone that plays an important role in plant defense against insect pests. Genes coding for the 4 key enzymes involved in the production of JA viz., LOX, AOS, AOC, and OPR are identified in C. canephora (robusta coffee) by

  14. Diversity and communities of foliar endophytic fungi from different agroecosystems of Coffea arabica L. in two regions of Veracruz, Mexico.

    Science.gov (United States)

    Saucedo-García, Aurora; Anaya, Ana Luisa; Espinosa-García, Francisco J; González, María C

    2014-01-01

    Over the past 20 years, the biodiversity associated with shaded coffee plantations and the role of diverse agroforestry types in biodiversity conservation and environmental services have been topics of debate. Endophytic fungi, which are microorganisms that inhabit plant tissues in an asymptomatic manner, form a part of the biodiversity associated with coffee plants. Studies on the endophytic fungi communities of cultivable host plants have shown variability among farming regions; however, the variability in fungal endophytic communities of coffee plants among different coffee agroforestry systems is still poorly understood. As such, we analyzed the diversity and communities of foliar endophytic fungi inhabiting Coffea arabica plants growing in the rustic plantations and simple polycultures of two regions in the center of Veracruz, Mexico. The endophytic fungi isolates were identified by their morphological traits, and the majority of identified species correspond to species of fungi previously reported as endophytes of coffee leaves. We analyzed and compared the colonization rates, diversity, and communities of endophytes found in the different agroforestry systems and in the different regions. Although the endophytic diversity was not fully recovered, we found differences in the abundance and diversity of endophytes among the coffee regions and differences in richness between the two different agroforestry systems of each region. No consistent pattern of community similarity was found between the coffee agroforestry systems, but we found that rustic plantations shared the highest number of morphospecies. The results suggest that endophyte abundance, richness, diversity, and communities may be influenced predominantly by coffee region, and to a lesser extent, by the agroforestry system. Our results contribute to the knowledge of the relationships between agroforestry systems and biodiversity conservation and provide information regarding some endophytic fungi and

  15. Performance of coffee origin and genotype in organoleptic and physical quality of arabica coffee in North Sumatra Province of Indonesia

    Science.gov (United States)

    Malau, Sabam; Siagian, Albiner; Sirait, Bilter; Pandiangan, Samse

    2017-09-01

    The objective of this research was to determine effect of coffee origin and genotype on organoleptic and physical quality of Arabica coffea L. growing in North Sumatra. Seven districts treated as origins and 28 genotypes were chosen. The research was conducted with nested design with 3 factors. Organoleptic parameters were fragrance/aroma, flavor, aftertaste, acidity, body, uniformity, balance, clean cup, sweetness, overall and total score. Physical quality was green bean weight. The results revealed that origins affected significantly organoleptic quality. Coffee from Dairi showed the highest total score (90,82). Genotypes were significantly different in organoleptic quality. Genotype Da17, Da18, Da19, Da20 and Hu4 had the best total score (89,85 -91,68). Total score did not correlate with green bean weight but had positive correlation with altitude. Among organoleptic parameters, acidity was more significant for total score (r2 = 0,836). Altitude had more effect on acidity (r2 = 0,486).

  16. High-throughput metabolic profiling of diverse green Coffea arabica beans identified tryptophan as a universal discrimination factor for immature beans.

    Science.gov (United States)

    Setoyama, Daiki; Iwasa, Keiko; Seta, Harumichi; Shimizu, Hiroaki; Fujimura, Yoshinori; Miura, Daisuke; Wariishi, Hiroyuki; Nagai, Chifumi; Nakahara, Koichi

    2013-01-01

    The maturity of green coffee beans is the most influential determinant of the quality and flavor of the resultant coffee beverage. However, the chemical compounds that can be used to discriminate the maturity of the beans remain uncharacterized. We herein analyzed four distinct stages of maturity (immature, semi-mature, mature and overripe) of nine different varieties of green Coffea arabica beans hand-harvested from a single experimental field in Hawaii. After developing a high-throughput experimental system for sample preparation and liquid chromatography-mass spectrometry (LC-MS) measurement, we applied metabolic profiling, integrated with chemometric techniques, to explore the relationship between the metabolome and maturity of the sample in a non-biased way. For the multivariate statistical analyses, a partial least square (PLS) regression model was successfully created, which allowed us to accurately predict the maturity of the beans based on the metabolomic information. As a result, tryptophan was identified to be the best contributor to the regression model; the relative MS intensity of tryptophan was higher in immature beans than in those after the semi-mature stages in all arabica varieties investigated, demonstrating a universal discrimination factor for diverse arabica beans. Therefore, typtophan, either alone or together with other metabolites, may be utilized for traders as an assessment standard when purchasing qualified trading green arabica bean products. Furthermore, our results suggest that the tryptophan metabolism may be tightly linked to the development of coffee cherries and/or beans.

  17. Impacts of leaf age and heat stress duration on photosynthetic gas exchange and foliar nonstructural carbohydrates in Coffea arabica

    Science.gov (United States)

    Danielle E. Marias; Frederick C. Meinzer; Christopher Still

    2017-01-01

    Given future climate predictions of increased temperature, and frequency and intensity of heat waves in the tropics, suitable habitat to grow ecologically, economically, and socially valuable Coffea arabica is severely threatened. We investigated how leaf age and heat stress duration impact recovery from heat stress in C. arabica...

  18. An endoplasmic reticulum-localized Coffea arabica BURP domain-containing protein affects the response of transgenic Arabidopsis plants to diverse abiotic stresses.

    Science.gov (United States)

    Dinh, Sy Nguyen; Kang, Hunseung

    2017-11-01

    The Coffea arabica BURP domain-containing gene plays an important role in the response of transgenic Arabidopsis plants to abiotic stresses via regulating the level of diverse proteins. Although the functions of plant-specific BURP domain-containing proteins (BDP) have been determined for a few plants, their roles in the growth, development, and stress responses of most plant species, including coffee plant (Coffea arabica), are largely unknown. In this study, the function of a C. arabica BDP, designated CaBDP1, was investigated in transgenic Arabidopsis plants. The expression of CaBDP1 was highly modulated in coffee plants subjected to drought, cold, salt, or ABA. Confocal analysis of CaBDP1-GFP fusion proteins revealed that CaBDP1 is localized in the endoplasmic reticulum. The ectopic expression of CaBDP1 in Arabidopsis resulted in delayed germination of the transgenic plants under abiotic stress and in the presence of ABA. Cotyledon greening and seedling growth of the transgenic plants were inhibited in the presence of ABA due to the upregulation of ABA signaling-related genes like ABI3, ABI4, and ABI5. Proteome analysis revealed that the levels of several proteins are modulated in CaBDP1-expressing transgenic plants. The results of this study underscore the importance of BURP domain proteins in plant responses to diverse abiotic stresses.

  19. Simple Sequence Repeat Analysis of Selected NSIC-registered Coffee Varieties in the Philippines

    OpenAIRE

    Daisy May C. Santos; Carla Francesca F. Besa; Angelo Joshua A. Victoria

    2016-01-01

    Coffee (Coffea sp.) is an important commercial crop worldwide. Three species of coffee are used as beverage, namely Coffea arabica, C. canephora, and C. liberica. Coffea arabica L. is the most cultivated among the three coffee species due to its taste quality, rich aroma, and low caffeine content. Despite its inferior taste and aroma, C. canephora Pierre ex A. Froehner, which has the highest caffeine content, is the second most widely cultivated because of its resistance to coffee diseases. O...

  20. Composition of the root mycorrhizal community associated with Coffea arabica in Fifa Mountains (Jazan region, Saudi Arabia).

    Science.gov (United States)

    Mahdhi, Mosbah; Tounekti, Taieb; Al-Turki, Turki Ali; Khemira, Habib

    2017-08-01

    Arbuscular mycorrhizal fungi (AMF) constitute a key functional group of soil biota that can greatly contribute to crop productivity and ecosystem sustainability. They improve nutrient uptake and enhance the ability of plants to cope with abiotic stresses. The presence of AMF in coffee (Coffea arabica L.) plant roots have been reported in several locations but not in Saudi Arabia despite the fact that coffee has been in cultivation here since ancient times. The objective of the present study was to investigate the diversity of AMF communities colonizing the roots of coffee trees growing in two sites of Fifa Mountains (south-west Saudi Arabia): site 1 at 700 m altitude and site 2 at 1400 m. The AMF large subunit rDNA regions (LSU) were subjected to nested PCR, cloning, sequencing, and phylogenetic analysis. Microscopic observations indicated higher mycorrhizal intensity (24.3%) and spore density (256 spores/100 g of soil) in site 2 (higher altitude). Phylogenetic analysis revealed 10 phylotypes, six belonging to the family Glomeraceae, two to Claroideoglomercea, one to Acaulosporaceae and one to Gigasporaceae family. Glomus was the dominant genus at both sites and the genus Gigaspora was detected only at site 2. This is the first study reporting the presence of AMF in coffee roots and the composition of this particular mycorrhizal community in Saudi Arabia. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Comparative effect of coffee robusta and coffee arabica (Qahwa) on memory and attention.

    Science.gov (United States)

    Alharbi, Waheeb D M; Azmat, Aisha; Ahmed, Muhammad

    2018-04-13

    The comparative effects of coffee robusta and coffee arabica (Qahwa) on different attention and memory related assignments were measured in a double-blind study of 300 healthy young adult women who were randomly assigned to one of three different drinks: Group I (coffee robusta sachet dissolved in 100 ml of hot water): Group II (coffee arabica): and group III (100 ml water only). Cognitive function was assessed by standardized tests. Several monitoring cognitive tests and tasks were specifically chosen and performed to investigate the comparative effects of coffee robusta (CR) and coffee arabica (Qahwa; AC) on sleepiness (sleep and clear headed scale), attention (trail A & B, symbol digit, letter cancellation), general cognitive ability (stroop test) and memory (card test). Data was interpreted by analysis of variance (ANOVA). The present study revealed that coffee robusta has beneficial effects on attention, general cognitive ability and memory. Higher though non-significant cognitive scores were associated with coffee robusta consumption. Although, consumption of coffee arabica (Qahwa) has significant effects (P < 0.05) on sleepiness, attention, general cognitive ability and memory and it significantly improve reaction time and correct responses. Since different tasks were related to the sustained attention and working memory processes, results would suggest that coffee arabica (qahwa) could increase the memory and efficiency of the attentional system might be due to the presence of chlorogenic acids (CGA) which are found in less quantity in coffee robusta. However, more studies using larger samples and different tasks are necessary to better understand the effects of coffee robusta and arabica (Qahwa) on attention and memory.

  2. Bacillus species (BT42) isolated from Coffea arabica L. rhizosphere antagonizes Colletotrichum gloeosporioides and Fusarium oxysporum and also exhibits multiple plant growth promoting activity.

    Science.gov (United States)

    Kejela, Tekalign; Thakkar, Vasudev R; Thakor, Parth

    2016-11-18

    Colletotrichum and Fusarium species are among pathogenic fungi widely affecting Coffea arabica L., resulting in major yield loss. In the present study, we aimed to isolate bacteria from root rhizosphere of the same plant that is capable of antagonizing Colletotrichum gloeosporioides and Fusarium oxysporum as well as promotes plant growth. A total of 42 Bacillus species were isolated, one of the isolates named BT42 showed maximum radial mycelial growth inhibition against Colletotrichum gloeosporioides (78%) and Fusarium oxysporum (86%). BT42 increased germination of Coffee arabica L. seeds by 38.89%, decreased disease incidence due to infection of Colletotrichum gloeosporioides to 2.77% and due to infection of Fusarium oxysporum to 0 (p Fusarium oxysporum. The mechanism of action of inhibition of the pathogenic fungi found to be synergistic effects of secondary metabolites, lytic enzymes, and siderophores. The major inhibitory secondary metabolite identified as harmine (β-carboline alkaloids).

  3. Taxonomia de Coffea arabica L. VI: caracteres morfológicos dos haplóides

    Directory of Open Access Journals (Sweden)

    A. Carvalho

    1952-06-01

    monosperma, bem como as plantas bullata, podem ser encontradas em qualquer variedade de café, motivo por que não devem ser consideradas como variedades, mas, apenas, como haplóides ou poliplóides das variedades de que se originaram.Haploid plants were found in progenies of the following varieties of Coffea arabica : typica, bourbon, maragogipe, semperflorens, laurina, erecta, caturra and San Ramon. These haploids resemble the normal plants from which they have originated ; they are reduced in size, their branches are more slender and the leaves are narrower and thinner. Flowers are normal, smaller, with very low fertility, due to abnormal meiosis. The dominant genetic factors maragogipe (Mg, erecta (Er, caturra (Ct, and San Ramon, and the recessive factors semperflorens (sf and laurina (lr have the same phenotypical expression both in haploid and normal plants. The Br (bronze young leaves gene shows incomplete dominance, the heterozygous plants having light bronze tips; the haploid with a single dose of Br has also a light bronze color of the young leaves. In the presence of tt the Na gene is incompletely dominant the heterozygotes having the murta phenotype (ttNana. Haploids derived from bourbon (ttNaNa, therefore hemizygotes t Na, do not resemble murta but bourbon. Attention was called to pure lines obtained through duplication of chromosome number of haploids, for use in progeny tests, in order to measure the environmental variation, and also to determine mutation rate in C. arabica. Observation on meiosis and the results of genetic analysis have already pointed out that C. arabica is probably an allotetraploid of ancient origin ; this has been confirmed by the study of the haploids here described. Monosperma coffee plants with 22 somatic chromosomes, and the bullata types with 66 or 88 chromoeomes, should not be considered any more as varieties of C. arabica, but only as haploids or polyploids of the varieties from which they originated.

  4. Fontes e proporção de material orgânico para a produção de mudas de cafeeiro em tubetes Sources and proportions of organic components for production of coffee tree seedling (Coffea arabica L. in small plastic containers

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    Ricardo Dias

    2009-06-01

    Full Text Available Neste trabalho, objetivou-se avaliar a qualidade das mudas de cafeeiro (Coffea arabica L. produzidas em tubetes com substrato artificial suplementado com fontes de material orgânico em diferentes proporções. O experimento foi conduzido sob delineamento experimental de blocos casualizados em esquema fatorial 3 x 6, com quatro repetições em parcelas formadas por seis tubetes. O primeiro fator correspondeu às fontes de material orgânico (esterco bovino, húmus de minhoca e cama de peru e o segundo, suas proporções (0, 20, 40, 60, 80 e 100% no substrato artificial (BioPlant-café fertilizado com osmocote® (NPK 15-09-12. As características avaliadas foram: altura da muda, diâmetro de caule, número de pares de folhas, área foliar e massa seca da parte aérea e do sistema radicular. Pode-se concluir que o húmus de minhoca adicionado ao substrato artificial na proporção de 80% ou em uso exclusivo (100% aumentou a área foliar das mudas de cafeeiro e, como conseqüência proporcionou um maior acúmulo de massa seca da parte aérea. O uso de esterco bovino (acima de 30% e a cama de peru não alterou ou reduziu as características vegetativas das mudas de cafeeiro (altura da muda, diâmetro de caule, número de pares de folhas e área foliar e diminuiu o acúmulo de massa seca.This work aimed to evaluate the quality of coffee seedlings (Coffea arabica L. produced in small plastic containers with artificial substrate supplemented with sources of organic components in different proportions. The experiment was carried out using the randomized block design, in a 3 x 6 factorial structure, with four replications in plots composed of small plastic containers. The first factor corresponded to the sources (cattle manure, earthworm casting, and turkey manure and the second to the proportions (0, 20, 40, 60, 80, and 100% in the artificial substrate (BioPlant-café® fertilized with osmocote® (NPK 15-09-12. The characteristics evaluated were

  5. Meta-analysis for heritability estimates of vegetative and reproductive traits of Coffea arabica L.Meta-análise para estimativas de herdabilidade de caracteres vegetativos e reprodutivos de Coffea arabica L.

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    Danielle Pereira Baliza

    2012-08-01

    Full Text Available The compilation of informations resulting from independent studies has been difficulted in almost all scientific fields, mainly due to the great number of scientific papers published in recent years. As a result, summarizing information became a need. In this context, a meta-analysis was conducted with the objective of summarizing the estimates for the heritability for vegetative and reproductive traits of coffee (Coffea arabica L.. Heritability estimates were appraised regarding the following traits: average height of plant, average diameter of the canopy, vegetative vigor, production of processed coffee, yield and rust. The data regarding the heritability estimates are from scientific articles published in national and international journals, congress annals, PhD thesis and Master dissertations. The technique of meta-analysis summarized the estimates heritability from different studies and made possible to conclude that all of the appraised traits are highly inherited, reflecting the great genetic variety of coffee plants, and that is possible to reach satisfactory genetic gains in improvement programs in which those traits are evaluated. A compilação de informações advindas de estudos independentes tem sido dificultada em quase todos os campos da ciência, devido principalmente, ao grande número de trabalhos científicos publicados nos últimos anos. Assim, sumarizar informações tornou-se uma necessidade. Neste contexto, uma meta-análise foi conduzida com o objetivo de sistematizar as estimativas para a herdabilidade de caracteres vegetativos e reprodutivos de cafeeiros (Coffea arabica L.. Foram avaliadas as estimativas de herdabilidade referentes aos seguintes caracteres: altura média da planta, diâmetro médio da copa, vigor vegetativo, produção de café beneficiado, rendimento e resistência a ferrugem. Os dados referentes às estimativas de herdabilidade são provenientes de artigos científicos publicados em revistas

  6. Caracterização e dinâmica de colonização de Cladosporium cladosporioides (Fresen. de vries em frutos do cafeeiro (Coffea arabica L. Characterization and dynamic of colonization of Cladosporium cladosporioides (Fresen. de Vries in coffee fruits (Coffea arabica L.

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    Ricardo Tadeu Galvão Pereira

    2005-12-01

    Full Text Available A presença de fungos em associação natural com frutos do cafeeiro é considerada um fator importante influenciando a qualidade do café. A influência negativa de algumas espécies de Aspergillus é conhecida, comprometendo inclusive a segurança do produto. Os relatos de fungos influenciando positivamente a qualidade se resumem à ocorrência de Cladosporium sp. associados a grãos que originaram cafés de boa qualidade, porém informações exatas sobre a espécie e a sua dinâmica no campo são escassas. Objetivando caracterizar a espécie associada ao cafeeiro e sua dinâmica de colonização, 18 isolados de Cladosporium foram caracterizados e identificados. A dinâmica de colonização do fungo nas comunidades externa e interna do fruto do cafeeiro foi estudada ao longo do período de desenvolvimento do fruto. A espécie associada ao cafeeiro foi identificada como Cladosporium cladosporioides (Fresen. de Vries. A dinâmica do fungo é característica de um fungo saprófita encontrado em intensidade máxima quando os frutos estão nos estágios de cereja.The natural occurrence of fungi in coffee fruits is considered an important factor influencing the quality of coffee. The negative effect of some Aspergillus species in coffee, which can also affect safety of the product, is well known. Otherwise, there are reports describing the positive influence of fungi in coffee quality, but they are limited to the occurrence of Cladosporium sp. in fruits, and its correlation with a product of good quality. Indeed, the exact information about the species involved and dynamics of colonization are not available. The objective of this work was to identify and characterize the species of Cladosporium, detected in coffee fruits, and study the dynamics of colonization of the fruits during the maturation process. The species found on coffee fruits was identified as Cladosporium cladosporioides (Fresen. de Vries. and the dynamics of colonization showed the

  7. Physical and sensory quality of Java Arabica green coffee beans

    Science.gov (United States)

    Sunarharum, W. B.; Yuwono, S. S.; Pangestu, N. B. S. W.; Nadhiroh, H.

    2018-03-01

    Demand on high quality coffee for consumption is continually increasing not only in the consuming countries (importers) but also in the producing countries (exporters). Coffee quality could be affected by several factors from farm to cup including the post-harvest processing methods. This research aimed to investigate the influence of different post-harvest processing methods on physical and sensory quality of Java Arabica green coffee beans. The two factors being evaluated were three different post-harvest processing methods to produce green coffee beans (natural/dry, semi-washed and fully-washed processing) under sun drying. Physical quality evaluation was based on The Indonesian National Standard (SNI 01-2907-2008) while sensory quality was evaluated by five expert judges. The result shows that less defects observed in wet processed coffee as compared to the dry processing. The mechanical drying was also proven to yield a higher quality green coffee beans and minimise losses.

  8. Effect of roasting on the carbohydrate composition of Coffea arabica beans.

    NARCIS (Netherlands)

    Oosterveld, A.; Voragen, A.G.J.; Schols, H.A.

    2003-01-01

    Coffee beans (arabica) with different degrees of roast were sequentially extracted with water (90 °C, 1 h), water (170 °C, 30 min), and 0.05 M NaOH (0 °C, 1 h). The amount and composition of polysaccharides, oligosaccharides and monosaccharides in the extracts and residues were analyzed. The results

  9. Anatomia e desenvolvimento ontogenético de Coffea arabica L. var. typica Cramer Anatomy and ontogenetical development of Coffea arabica L. var. typica Cramer

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    D. M. Dedecca

    1957-01-01

    Full Text Available O presente estudo anatômico de Coffea arabica L. var. typica Cramer tem por finalidade fornecer informações básicas necessárias ao estudo da anatomia comparada das principais espécies e variedades de cafeeiros, cultivadas no Estado de São Paulo. Nesta primeira contribuição o autor realiza o estudo anatômico detalhado dos órgãos vegetativos e reprodutivos da variedade typica, não se limitando apenas à anatomia descritiva dos diversos órgãos, mas também, sempre que possível, discutindo o desenvolvimento ontogenético das diversas partes do cafeeiro. No estudo da raíz e do caule procurou-se estabelecer a duração do desenvolvimento primário, assinalando o local de aparecimento, primeiramente do câmbio vascular e, posteriormente, do felógeno ou câmbio suberoso. Na discussão da anatonia das folhas mereceu especial atenção o estudo das domácias, sua morfologia e possível função. As flores são estudadas detalhadamente nos seus diversos elementos. Nos capítulos referentes à anatomia do fruto e da semente, além do estudo puramente descritivo das suas estruturas são ainda discutidas as diversas modificações verificadas durante o desenvolvimento do ovário e dos óvulos, respectivamente em fruto e sementes.The knowledge of the anatomy of Coffea arabica L. var. typica Cramer should be considered as a basic contribution to the comparative study of the anatomy of coffee species and varieties cultivated in the State of São Paulo, Brazil. The detailed investigations carried out on the vegetative and reproductive organs of the coffee plant revealed the following. The roots at the end of the primary growth present a protostelic, poliarch (6, 7, 8, 9, 11 primary xylem bundles, and exarch structure, the following tissues or zone of tissues being visible: root cap, epidermis, exodermis, cortex, endodermis, pericycle, primary phloem and primary xylem. This primary growth has a very short duration and is very soon followed by the

  10. Incidência de ocratoxina A em diferentes frações do café (Coffea arabica L.: bóia, mistura e varrição após secagem em terreiros de terra, asfalto e cimento Incidence of ochratoxin A in fraction diferents coffee beans (Coffea Arabica L: "boia", mixes and "varrição"

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    Luís Roberto Batista

    2007-06-01

    Full Text Available A incidência de ocratoxina A foi estudada em café mistura, bóia e varrição secas em três tipos de terreiro: terra, cimento e asfalto. Foram analisadas 238 amostras coletadas em 11 municípios da região sul do Estado de Minas Gerais, sendo 35 bóia, 97 - mistura e 106 varrição. Das amostras analisadas, em 40% não foi detectada a presença de ocratoxina A, em 31%, foram detectadas a presença de ocratoxina A em níveis que variaram de 0,1 a 5,0 µg/Kg de café. Estes resultados demonstram que 169 amostras (71% analisadas estariam dentro dos limites em estudo da Legislação Européia que regulamenta a concentração máxima de ocratoxina A em grãos de café torrado. As espécies de Aspergillus identificadas como produtoras de ocratoxina A foram Aspergillus ochraceus, A. sclerotiorum e A. sulphureus. Os níveis de contaminação de ocratoxina A em grãos de café foram maiores na fração varrição e nas frações bóia e mistura, secas em terreiro de terra. Os resultados deste estudo concluem que o terreiro de terra aumenta o risco de contaminação com ocratoxina A em grãos de café. A fração varrição devido aos riscos de exposição a ocratoxina A, deve ser reduzida através da adoção de boas práticas agrícolas e não ser utilizada para fins de consumo humano e animal.The ochratoxin incidence was studied in coffee it mixes, it "bóia" and "varrição" dry in three yard types: earth, cement and asphalt. 238 samples were analyzed collected in 11 municipal districts of the south of Minas Gerais state, being 35 "bóia", 97 - it mixes and 106 varrição. Of the analyzed samples, in 40% the ochratoxin A presence it was not detected, in 74 samples, 31%, ochratoxin A presence were detected the in levels that varied from 0,1 to 5,0 µg/Kg of coffee beans. These results demonstrate that in 169 samples (71% analyzed they would be inside of the limits in study of the European Legislation that regulates the maximum concentration of

  11. Selection of arabica coffee progenies tolerant to heat stress

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    Alexsandro Lara Teixeira

    2015-07-01

    Full Text Available Due to high temperatures, practically all coffee farms in the state of Rondonia are of the C. canephora species. Thus, importing arabica coffee from other states becomes necessary for composition of blends, as well as for the specialty or gourmet coffee market. The purpose of this study was to select arabica coffee genotypes that exhibit satisfactory agronomic performance under high temperature conditions. The experiment was conducted in OuroPreto do Oeste, RO, Brazil, with mean annual temperature of 25.8°C and mean annual rainfall of 2300mm year-1. The experiment was composed of 114 arabica coffee genotypes, with 103 progenies and eleven control cultivars, provided by EPAMIG. A randomized block experimental design was used with three replications, spacing of 3.0x1.0 meters and five plants per plot. All the crop seasons showed significant difference for the green coffee yield trait. In joint analysis, significant differences were detected among progenies and control cultivars. In the average of the four harvests, green coffee yield was 32.38 bags ha-1. The cultivars 'CatuaíVermelho IAC 15', 'Obatã IAC 1669-20' and 'Catucaí Amarelo 2SLCAK' stood out, achieving yields greater than 40 bags ha-1. The gain obtained from selection was 14.33 bags ha-1, which is equivalent to an increase of 44.04% in production of green coffee. The progeny H514-7-10-6-2-3-9 stood out with an average yield of 51.20 bags ha-1. In regard to maturation cycle, 56% of the progenies were classified as early maturity and 44% as medium maturity. Late maturity genotypes were not observed

  12. Agrobacterium-mediated genetic transformation of Coffea arabica (L. is greatly enhanced by using established embryogenic callus cultures

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    Lashermes Philippe

    2011-05-01

    Full Text Available Abstract Background Following genome sequencing of crop plants, one of the main challenges today is determining the function of all the predicted genes. When gene validation approaches are used for woody species, the main obstacle is the low recovery rate of transgenic plants from elite or commercial cultivars. Embryogenic calli have frequently been the target tissue for transformation, but the difficulty in producing or maintaining embryogenic tissues is one of the main problems encountered in genetic transformation of many woody plants, including Coffea arabica. Results We identified the conditions required for successful long-term proliferation of embryogenic cultures in C. arabica and designed a highly efficient and reliable Agrobacterium tumefaciens-mediated transformation method based on these conditions. The transformation protocol with LBA1119 harboring pBin 35S GFP was established by evaluating the effect of different parameters on transformation efficiency by GFP detection. Using embryogenic callus cultures, co-cultivation with LBA1119 OD600 = 0.6 for five days at 20 °C enabled reproducible transformation. The maintenance conditions for the embryogenic callus cultures, particularly a high auxin to cytokinin ratio, the age of the culture (optimum for 7-10 months of proliferation and the use of a yellow callus phenotype, were the most important factors for achieving highly efficient transformation (> 90%. At the histological level, successful transformation was related to the number of proembryogenic masses present. All the selected plants were proved to be transformed by PCR and Southern blot hybridization. Conclusion Most progress in increasing transformation efficiency in coffee has been achieved by optimizing the production conditions of embryogenic cultures used as target tissues for transformation. This is the first time that a strong positive effect of the age of the culture on transformation efficiency was demonstrated. Our

  13. Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures

    Science.gov (United States)

    2011-01-01

    Background Following genome sequencing of crop plants, one of the main challenges today is determining the function of all the predicted genes. When gene validation approaches are used for woody species, the main obstacle is the low recovery rate of transgenic plants from elite or commercial cultivars. Embryogenic calli have frequently been the target tissue for transformation, but the difficulty in producing or maintaining embryogenic tissues is one of the main problems encountered in genetic transformation of many woody plants, including Coffea arabica. Results We identified the conditions required for successful long-term proliferation of embryogenic cultures in C. arabica and designed a highly efficient and reliable Agrobacterium tumefaciens-mediated transformation method based on these conditions. The transformation protocol with LBA1119 harboring pBin 35S GFP was established by evaluating the effect of different parameters on transformation efficiency by GFP detection. Using embryogenic callus cultures, co-cultivation with LBA1119 OD600 = 0.6 for five days at 20 °C enabled reproducible transformation. The maintenance conditions for the embryogenic callus cultures, particularly a high auxin to cytokinin ratio, the age of the culture (optimum for 7-10 months of proliferation) and the use of a yellow callus phenotype, were the most important factors for achieving highly efficient transformation (> 90%). At the histological level, successful transformation was related to the number of proembryogenic masses present. All the selected plants were proved to be transformed by PCR and Southern blot hybridization. Conclusion Most progress in increasing transformation efficiency in coffee has been achieved by optimizing the production conditions of embryogenic cultures used as target tissues for transformation. This is the first time that a strong positive effect of the age of the culture on transformation efficiency was demonstrated. Our results make Agrobacterium

  14. Effect of CO2 on somatic embryos development Coffea arabica L. cv. ‘Caturra rojo’ and Clematis tangutica K.

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    Raúl Barbon

    2016-07-01

    Full Text Available Studies to optimize somatic embryogenesis have traditionally focused on the components of the culture medium but little other in vitro environment factors have been analyzed such as the composition of the gaseous atmosphere. The objective of this work was to determine the influence of CO2 on the development of the somatic embryo during the transition from the globular to the torpedo stage. The research was carried out on two model species for somatic embryogenesis that they are developed in different climatic zones: Coffea arabica L. cv. ‘Caturra rojo’ and Clematis tangutica K. Three CO2 concentrations (2.5, 5.0 and 10.0% combined with 21% O2 and two controls (passive exchange and forced ventilation were used. The effect of CO2 on the differentiation of somatic embryos from globular to torpedo stage in coffee and clematis was demonstrated, since in the treatments with passive exchange, where there was accumulation of CO2, the differentiation of somatic embryos was superior to treatments with forced ventilation. With 5.0% CO2 the process of differentiation of the embryos in the globular stage was stimulated, because in the treatment with this concentration of CO2 for coffee and clematis the highest proportion of embryos in torpedo stages and low levels of malformation were obtained.   Keywords: carbon dioxide, differentiation, in vitro environment, somatic embryogenesis

  15. Incidence and distribution of filamentous fungi during fermentation, drying and storage of coffee (Coffea arabica L. beans Incidência e distribuição de fungos filamentosos durante a fermentação, secagem e armazenamento de frutos e grãos de café (Coffea arabica L.

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    Cristina Ferreira Silva

    2008-09-01

    Full Text Available The objective of this work was to isolate and characterize filamentous fungi present in different stages of harvest, fermentation, drying and storage of coffee beans processed by natural method. The cherries were hand-picked and then placed on a cement drying platform where they remained until reached 11% of humidity. Microbial counts were found in all samples during fermentation and drying of the coffee beans. Counts of fungi in the coffee cherries collected from the tree (time 0 were around 1.5 x 10³ CFU/g. This number increased slowly during the fermentation and drying reaching values of 2 x 10(5 CFU/g within 22 days of processing. Two hundred and sixty three isolates of filamentous fungi were identified. The distribution of species during fermentation and drying was very varied while there was a predominance of Aspergillus species during storage period. The genera found were Pestalotia (4, Paecelomyces (4, Cladosporium (26, Fusarium (34, Penicillium (81 and Aspergillus (112 and comprised 38 different species.O objetivo deste estudo foi isolar e caracterizar fungos filamentosos presentes em diferentes estágios de beneficiamento de café processado pelo método natural, incluindo: colheita, fermentação, secagem e armazenamento. O café cereja foi colhido manualmente e então colocado em uma plataforma de cimento, onde permaneceu até atingir 11% de umidade. A contagem microbiana foi realizada em todas as amostras durante a fermentação e secagem do café. A população de fungos filamentosos no café cereja ainda nos pés (tempo 0 foi em torno de 1,5 x 10³ UFC/g. Este número aumentou vagarosamente durante a fermentação e secagem, alcançando valores de 2 x 10(5 UFC/g em 22 dias do processamento. Duzentos e sessenta e três isolados de fungos filamentosos foram identificados. A distribuição das espécies durante fermentação e secagem foi bastante variada, mas no armazenamento dos grãos ocorreu o predomínio de espécies de

  16. RUMINAL DEGRADATION KINETIC PARAMETERS OF COFFEE HULLS (Coffea arabica, L. TREATED WITH SODIUM HYDROXIDE (NAOH PARÂMETROS CINÉTICOS DA DEGRADAÇÃO RUMINAL DA CASCA DE CAFÉ (Coffea arabica, L. TRATADA COM HIDRÓXIDO DE SÓDIO (NAOH

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    Luciano Fernandes de Sousa

    2008-04-01

    Full Text Available The objective of this work was to evaluate dry matter ruminal degradation kinetics of coffee hulls treated with increasing sodium hydroxide quantities. Two rumen fistulated cows were used to incubate samples in nylon bags for 12. 24, 36. 48 and 72 hours. Four ruminal incubation periods were used, in a complete randomized block design. Coffee hulls were treated with 0%, 3%, 6% and 9% of sodium hydroxide (dry matter basis, corresponding to treatments T1 to t4 respectively. Experimental results were compared using Tukey test, at 5% probability level, as follows for treatments 1 to 4 respectively: soluble fraction (9.35d; 17.65c; 31.93b; 32.28a, de (34.40d; 40.50c; 43.28b; 50.35a, potential degradability (44.33d; 50.33c; 52.35b; 57.70a and lag time in hours (4.03a; 3.93a; 4.33a; 2.55a. The results indicate that increasing the levels of NaOH in the coffee hulls treatments increased significantly their ruminal solubility, as well as their effective and potential degradabilities. However that increase had no effect upon coffee hulls lag time in the rumen.KEY WORDS: by-product feedstuffs, rumen degradability, ruminant.

    O objetivo deste trabalho foi o de determinar a cinética de degradação ruminal da matéria seca da casca de café, tratada com diferentes quantidades de hidróxido de sódio. Utilizaram-se duas vacas fistuladas no rúmen, incubando-se as amostras em sacolas de náilon por 12, 24, 36, 48 e 72 horas, por quatro rodadas seqüenciais, sendo que cada uma destas representou um bloco, dentro de um delineamento de blocos inteiramente casualizados. Tratou-se a casca de café com 0%, 3%, 6% e 9 % de hidróxido de sódio (base seca constituindo assim os tratamentos t1

  17. Method Development and Validation for the Determination of Caffeine: An Alkaloid from Coffea arabica by High-performance Liquid Chromatography Method.

    Science.gov (United States)

    Naveen, P; Lingaraju, H B; Deepak, M; Medhini, B; Prasad, K Shyam

    2018-01-01

    The present study was investigated to develop and validate a reversed phase high performance liquid chromatography method for the determination of caffeine from bean material of Coffee arabica. The separation was achieved on a reversed-phase C18 column using a mobile phase composed of water: methanol (50:50) at a flow rate of 1.0 mlmin-1. The detection was carried out on a UV detector at 272 nm. The developed method was validated according to the requirements for International Conference on Harmonisation (ICH) guidelines, which includes specificity, linearity, precision, accuracy, limit of detection and limit of quantitation. The developed method validates good linearity with excellent correlation coefficient (R2 > 0.999). In repeatability and intermediate precision, the percentage relative standard deviation (% RSD) of peak area was less than 1% shows high precision of the method. The recovery rate for caffeine was within 98.78% - 101.28% indicates high accuracy of the method. The low limit of detection and limit of quantitation of caffeine enable the detection and quantitation of caffeine from C. arabica at low concentrations. The developed HPLC method is a simple, rapid, precisely, accurately and widely accepted and it is recommended for efficient assays in routine work. A simple, accurate, and sensitive high-performance liquid chromatography (HPLC) method for caffeine from Coffea arabica has been developed and validated. The developed HPLC method was validated for linearity, specificity, precision, recovery, limits of detection, and limits of quantification by the International Conference on Harmonization guidelines. The results revealed that the proposed method is highly reliable. This method could be successfully applied for routine quality work analysis. Abbreviation Used: C. arabica : Coffee arabica, ICH: International Conference on Harmonisation, % RSD: Percentage Relative Standard Deviation, R2: Correlation Coefficient, ppm: Parts per million, LOD: Limits

  18. Análise comparativa das características da serrapilheira e do solo em cafezais (Coffea arabica L. cultivados em sistema agroflorestal e em monocultura, na Zona da Mata MG Comparative analysis of litter and soil characteristics under coffee (Coffea arabica L. crop in agroforestry and monoculture systems

    Directory of Open Access Journals (Sweden)

    Mônica Matoso Campanha

    2007-10-01

    Full Text Available O aporte de serrapilheira em sistemas agroflorestais pode melhorar as características químicas e físicas do solo, diminuir a erosão e permitir a manutenção da umidade no solo por mais tempo. Isso faz dele um sistema alternativo de produção de café em regiões com solos propensos à degradação. Este trabalho teve como objetivo realizar uma análise comparativa da quantidade e teor de nutrientes da serrapilheira e das características de fertilidade e do teor de umidade dos solos, em cafeeiros cultivados sob sistemas agroflorestal e solteiro. A pesquisa foi realizada na Zona da Mata mineira, durante o período compreendido entre janeiro de 1999 e maio de 2000. O sistema agroflorestal contribuiu com 6,1 Mg ha-1 ano-1 de matéria seca de serrapilheira, no entanto o solteiro aportou 4,5 Mg ha-1 ano-1, ressaltando-se que esta última apresentou teor mais elevado de macronutrientes. O solo do sistema agroflorestal exibiu maior teor de umidade de 20-40 cm, maior capacidade de troca de cátions e soma de bases trocáveis, maior teor de K, Ca, Mg, Cu e Zn em ambos os horizontes do solo e menor índice de saturação de alumínio e alumínio trocável na camada mais profunda do que o solo sob a monocultura. No cultivo solteiro, o solo apresentou maior teor de P e de matéria orgânica, tanto na camada superficial quanto na profunda.Coffee plants in agroforestry systems is an alternative to full sunlight cultivation, presenting the potential benefits of enhancing soil chemical and physical characteristics, reducing soil erosion, besides maintaining soil moisture for longer periods. This research aimed at comparing the quantity and nutrient concentration in the litter, soil fertility and soil moisture in coffee crops under full sunlight monocrop and in agroforestry systems, at the Zona da Mata, Minas Gerais, Brazil. The research was carried out between January 1999 and May 2000. The agroforestry system, contributed with 6.1 Mg ha-1 year-1 of

  19. Inibição in vitro de fungos toxigênicos por Pichia sp. e Debaryomyces sp. isoladas de frutos de café (Coffea arabica = In vitro inhibition of toxigenic filamentous fungi by Pichia sp. and Debaryomyces sp. isolates from coffee (Coffea arabica fruits

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    Darlê Martins Barros Ramos

    2010-07-01

    Full Text Available O café é um produto nacional com grande expressão para a economia brasileira. O uso excessivo de fungicidas tem levado a pesquisas sobre formas alternativas como o controle biológico. Objetivou-se avaliar o potencial antagônico de leveduras em co-cultivo com fungos filamentosos. Isolados das espécies Debaryomyces hansenii (UFLACF 889 e UFLACF 847 e Pichia anomala (UFLACF 710 e UFLACF 951 foram inoculados (103 a 106 células mL-1 com três espécies de fungos filamentosos, Aspergillus ochraceus, A. parasiticus e Penicillium roqueforti (103 a 106 esporos mL-1. A avaliação do crescimento micelial e a contagem de esporos foram realizadas durante 21 dias. Observou-se que o isolado UFLACF 889 apresentou, em média, maior efeito inibitório na produção de esporos de A. ochraceus (inibição de 82% e P. roqueforti (74%. O isolado UFLACF 710 inibiu a produção deesporos, em média, 60 e 75,6% de A. ochraceus e P. roqueforti, respectivamente. A. parasiticus foi o fungo mais resistente à inibição pelas leveduras. O crescimento micelial não foi inibido pela presença da levedura em co-cultivo. Portanto, pode-se concluir que leveduras em cultivo pareado com fungos filamentosos são capazes de inibir a produção de esporos e, potencialmente, diminuir a disseminação destes fungos no processamento de café.Coffee is a national product with great importance for the Brazilian economy. The excessive use of pesticides led to research for alternative forms, such as biological control. The objective of this work was to assess the potential antagonistic effect of yeast in dualculture with filamentous fungi. Isolates of Debaryomyces hansenii (UFLACF 889 and UFLACF 847 and Pichia anomala (UFLACF 710 and UFLACF 951 species were inoculated (103 to 106 células mL-1 with three species of filamentous fungi, Aspergillus ochraceus, A. parasiticus and Penicillium roqueforti (103 to 106 spores mL-1. The assessmentof mycelial growth and counting of spores was

  20. Analysis of coffee (Coffea arabica L.) performance in relation to radiation level and rate of nitrogen supply II. Uptake and distribution of nitrogen, leaf photosynthesis and first bean yields

    NARCIS (Netherlands)

    Bote, Adugna Debela; Zana, Zewdneh; Ocho, Fikre L.; Vos, Jan

    2018-01-01

    Natural supply of nitrogen is often limiting coffee production. From the viewpoints of growth and biomass production, adequate nitrogen supply is important. Growing coffee under full sunlight not only enhances potential yields but also increases demands for nitrogen fertilizer, the extent of which

  1. Identification of biochemical features of defective Coffea arabica L. beans.

    Science.gov (United States)

    Casas, María I; Vaughan, Michael J; Bonello, Pierluigi; McSpadden Gardener, Brian; Grotewold, Erich; Alonso, Ana P

    2017-05-01

    Coffee organoleptic properties are based in part on the quality and chemical composition of coffee beans. The presence of defective beans during processing and roasting contribute to off flavors and reduce overall cup quality. A multipronged approach was undertaken to identify specific biochemical markers for defective beans. To this end, beans were split into defective and non-defective fractions and biochemically profiled in both green and roasted states. A set of 17 compounds in green beans, including organic acids, amino acids and reducing sugars; and 35 compounds in roasted beans, dominated by volatile compounds, organic acids, sugars and sugar alcohols, were sufficient to separate the defective and non-defective fractions. Unsorted coffee was examined for the presence of the biochemical markers to test their utility in detecting defective beans. Although the green coffee marker compounds were found in all fractions, three of the roasted coffee marker compounds (1-methylpyrrole, 5-methyl- 2-furfurylfuran, and 2-methylfuran) were uniquely present in defective fractions. Published by Elsevier Ltd.

  2. Produção de biogás no tratamento dos efluentes líquidos do processamento de Coffea arabica L. em reator anaeróbico UASB para o potencial aproveitamento na secagem do café Biogas production in the treatment of Coffea arabica L. processing wastewaters in UASB anaerobic reactor for the potential use in the coffee drying

    Directory of Open Access Journals (Sweden)

    Marco Antônio Calil Prado

    2008-06-01

    Full Text Available Estudou-se a produção de biogás proveniente do tratamento das águas residuárias do processamento por via úmida do café (ARC coco em sistema de tratamento anaeróbio em escala laboratorial. O sistema foi composto de um tanque de acidificação e equalização (TAE, um reator anaeróbio de manta de lodo e fluxo ascendente (UASB, uma lagoa aerada facultativa (LAF, um equalizador de pressão e um gasômetro. O tratamento foi realizado durante 190 dias e o pH foi controlado por certos períodos de tempo, pela adição de NaOH no TAE ou no reator UASB. No reator UASB, os valores máximos e mínimos obtidos na entrada foram de 235 a 7.064 mg.L-1 para DQO; 200 a 3.913 mg.L-1 para DBO5, 500 a 11.153 mg.L-1 para STV e 4,57 a 7,75 para o pH. Na saída do reator UASB, os valores foram de 39 a 2.333 mg.L-1 para DQO; 15 a 1.300 mg.L-1 para DBO5, 272 a 2.749 mg.L-1 para STV e 6,16 a 7,93 para o pH. Os valores mínimos e máximos de vazão afluente foram de 0,18 a 1,56 L.h-1. O biogás apresentou uma produção teórica de 0,545 a de 0,602 m³.kg-1DBO5 e porcentagem de metano de 48,60 a 68,14%.It was studied the biogas production through the treatment of the wet processing coffee wastewaters (ARC in an anaerobic treatment system in laboratorial scale. The system used was composed by one acidification and equalization tank (TAE, one anaerobic upflow sludge blanket reactor (UASB, one facultative aerated pond, one equalization tank and one gas tank. The treatment was carried out for 190 days and the pH was controlled for some periods by adding NaOH inside of the TAE or in the UASB. In the UASB reactor the maximum and minimum values obtained in the inlet were 235 to 7064 mg.L-1 for COD; 200 to 3913 mg.L-1 for BOD5, 500 to 11.153 mg.L-1 for TVS and 4,57 to 7,75 for pH. In the outlet of the UASB, the values were 39 to 2333 mg.L-1 for COD; 15 to 1300 mg.L-1 for BOD5, 272 to 2749 mg.L-1 for TVS and 6,16 to 7,93 for pH. The minimum and maximum values of the inlet

  3. DNA pyrosequencing evidence for large diversity differences between natural and managed coffee mycorrhizal fungal communities

    OpenAIRE

    De Beenhouwer , Matthias; Muleta , Diriba; Peeters , Bram; Van Geel , Maarten; Lievens , Bart; Honnay , Olivier

    2015-01-01

    International audience; Arabica coffee is a major agricultural commodity worldwide, representing 60 % of the world’s coffee production. Arabica coffee is cultivated in more than 36 countries and is a key cash crop for many developing countries. Despite the coffee’s huge economic importance, there is very limited knowledge on the association of arbuscular mycorrhizal fungi with coffee roots. Therefore, we assessed the mycorrhizal diversity and community composition in Arabica coffee (Coffea ar...

  4. Tolerance of Coffea arabica L. seeds to sub zero temperatures

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    Stefania Vilas Boas Coelho

    Full Text Available ABSTRACT Preservation of the quality of coffee seeds is hindered by their intermediate behavior in storage. However, long-term storage at sub zero temperatures may be achieved by adjusting the water content of the seeds. The aim of this study was to evaluate the tolerance of coffee seeds to freezing, in relation to physiological and enzymatic modifications. Coffee seeds were dried in two manners, rapid and slow, to water contents of interest, 0.67, 0.43, 0.25, 0.18, 0.11, and 0.05 g H2O g-¹ dw (dry basis. After drying, the seeds were stored at a temperature of -20 ºC and of 86 ºC for 24 hours and for 12 months, and then compared to seeds in cold storage at 10 ºC. The seeds were evaluated through calculation of percentage of normal seedlings, percentage of seedlings with expanded cotyledonary leaves, dry matter of roots and of hypocotyls, and viability of embryos in the tetrazolium test. Expression of the enzymes superoxide dismutase, catalase, and peroxidase were evaluated by means of electrophoretic analysis. Only seeds dried more slowly to 0.18 g H2O g-1 dw present relative tolerance to storing at -20 °C for 12 months. Coffee seeds do not tolerate storage at a temperature of -86 ºC for 12 months. Water contents below 0.11g H2O g-¹ dw and above 0.43 g H2O g-¹ dw hurt the physiological quality of coffee seeds, regardless of the type of drying, temperature, and storage period. Coffee seed embryos are more tolerant to desiccation and to freezing compared to whole seeds, especially when the seeds are dried to 0.05 g H2O g-¹ dw. The catalase enzyme can be used as a biochemical marker to study tolerance to freezing in coffee seeds.

  5. Physical and Flavor Profiles of Arabica Coffee as Affected by Cherry Storage Before Pulping

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    Yusianto .

    2014-08-01

    Full Text Available Harvesting and pulping process of coffee cherry in the same day is inaccesible. Storage of coffee cherry before pulping was carried out incorrectly. Some storage treatments before pulping of Arabica coffee cherry had been examined at Indonesian Coffee and Cocoa Research Institute using Arabica coffee cherries from Andungsari Experimental Garden, Bondowoso, East Java. Treatments of the experiment were method and period of cherry storage. Methods of coffee cherry storage were put in plastic sacks; immerse in water, without water replacement; and immerse in water with daily water replacement. Period of coffee cherry storage were 0, 1, 2, 3, 4, 5, 6, and 7 days. After storage treatments, the coffee cherries were pulped, fermented, washed, sundried, and dehulled. The experiment were carried out using randomized block design with three replications. Observation of coffee cherry during storage periods was done on the physical and temperature. Observation of the green coffee were done on the color dan bulk density. The green coffee were roasted at medium roast level for sensory analysis. Observation of roasting profile were out-turn, bulk density and pH of roasted coffee. Sensory analysis used Specialty Coffee Association of America method. Methods and period of cherry storage before pulping significanly influence on the cherry color, parchment color, green coffee color, and the flavor profile of Arabica coffee. Color of dry parchment changed to be red-brown becouse of cherry immersed in water for two days or more. In plastic sacks, Arabica coffee cherry may be stored only for two days, but underwater with or without water replacement, should be not more than five days. Green and sensory quality of Arabica coffee will be deteriorated after five days storage underwater. Coffee cherry storage immerse in water with daily replacing water may improve sensory quality of Arabica coffee.Key word: Arabica coffe, storage, pulping, flavor, physical

  6. Inheritance of Arabica Coffee Resistance to Radopholus similisCobb.

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    Retno Hulupi

    2007-05-01

    Full Text Available A research to get inheritance of Arabica coffee resistance to Radopholus similisnematode was done in screen house and laboratory of Indonesian Coffee and Cocoa Research Institute, also at endemic area of coffee plantation, using F1, F1 R and F2 crossing between BP 542 A(resistant x Andungsari 1 (susceptible with their reciprocal, and BP 542 A x Kartika 1. The purpose of this study that was conducted at seedling stage is to formulate a Strategy for Arabica coffee breeding to get resistant varieties to nematode. As the variables of resistance were weight of seedling biomass, percent of root weight deviation, number of root nematodes, number of soil nematodes, reproduction and percent of necrotic root. Using discriminant analysis and fastclus, those data variables were analyzed for genetic of resistance with Statistical Analysis System programme version 8. Genetic study on the inheritance of resistance to R. similiswas started with evaluation of homozigosity of BP 542 A was resistant parent. The result showed that BP 542 A was heterozygous. Therefore, segregation test could not be suggested with segregation pattern principals as Mendel proposed. Segregation test on BP 542 A showed that it was heterozygote and the resistance was controlled by single gene with complete dominant effect, so the progeny segregated in 75% resistant and 25% susceptible. The result of the test showed the absence of maternal effect for root weight deviation and percentage of necrotic root variables, which meant that no cytoplasmic inheritance was involved. Based on the test of segregation ratio, almost all of the resistance was not appropriate for monogenic and or digenic segregation pattern as expected due to non allelic gene interaction that caused epistasis. Key words: Inheritance, resistance, Arabica coffee, Radopholus similis.

  7. Proteomic analysis of apoplastic fluid of Coffea arabica leaves highlights novel biomarkers for resistance against Hemileia vastatrix

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    Leonor eGuerra-Guimarães

    2015-06-01

    Full Text Available A proteomic analysis of the apoplastic fluid (APF of coffee leaves was conducted to investigate the cellular processes associated with incompatible (resistant and compatible (susceptible Coffea arabica-Hemileia vastatrix interactions, during the 24-96 hai period. The APF proteins were extracted by leaf vacuum infiltration and protein profiles were obtained by 2-DE. The comparative analysis of the gels revealed 210 polypeptide spots whose volume changed in abundance between samples (control, resistant and susceptible during the 24-96 hai period. The proteins identified were involved mainly in protein degradation, cell wall metabolism and stress/defense responses, most of them being hydrolases (around 70%, particularly sugar hydrolases and peptidases/proteases. The changes in the APF proteome along the infection process revealed two distinct phases of defense responses, an initial/basal one (24-48 hai and a late/specific one (72-96 hai. Compared to susceptibility, resistance was associated with a higher number of proteins, which was more evident in the late/specific phase. Proteins involved in the resistance response were mainly, glycohydrolases of the cell wall, serine proteases and pathogen related-like proteins (PR-proteins, suggesting that some of these proteins could be putative candidates for resistant markers of coffee to H. vastatrix. Antibodies were produced against chitinase, pectin methylesterase, serine carboxypeptidase, reticuline oxidase and subtilase and by an immunodetection assay it was observed an increase of these proteins in the resistant sample. With this methodology we have identified proteins that are candidate markers of resistance and that will be useful in coffee breeding programs to assist in the selection of cultivars with resistance to H. vastatrix.

  8. Arabica Coffee Farming and Marketing Chain Analysis in Manggarai and EastManggarai Districts

    OpenAIRE

    Sophia Hartatri, Dhiany. Faila; de Rosari, Bernard

    2011-01-01

    Arabica coffee has a unique flavour and very potential market. The purpose of this study was to analyse Arabica coffee farming and to investigate its performance of marketing chains in Manggarai and East Manggarai Districts, Flores, East Nusa Tenggara Province. This research was conducted in 2008-2010 by interviewing coffee farmers and coffee buyers; using open and close questions. The number of respondents were 100 people in each district. The result showed that land holding per household fa...

  9. Genética de coffea VII: hereditariedade dos caracteres de coffea arabica L. var. maragogipe hort ex froehner

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    C. A. Krug

    1942-01-01

    Full Text Available A variedade maragogipe do Coffea arabica L. foi encontrada pela primeira vez por Crisógono José Fernandes, em 1870, no município baiano de Maragogipe onde, provavelmente, se originou por mutação. Desde 1933 esta variedade vem sendo estudada pela Secção de Genética do Instituto Agronômico do Estado de São Paulo, em Campinas, com o fim de se determinar a sua constituição genética. Muitas autofecundações, cruzamentos e back-crosses foram, então, realizados. Grande parte das plantas obtidas só puderam ser classificadas após a colheita do ano de 1940. Todas foram examinadas quanto à forma e dimensões das folhas e um grande número ainda quanto à forma e dimensões das flores, frutos e sementes. Verificou-se que o caráter maragogipe mostra dominância quase completa em F1, não sendo possivel uma separação das ciasses maragogipe puro e híbrido. Em F2, e nos back-crosses com as formas normais, obtiveram-se, respectivamente, relações de 3:1 e 1:1 entre plantas maragogipe e plantas normais, relações essas que demonstram que os caracteres do maragogipe são controlados por um único par de fatores genéticos dominantes, para os quais se propõe o símbolo Mg-Mg, derivado do próprio nome desta variedade.In the present article the results of the genetical analysis of the characters of the maragogipe variety of Coffea arabica L are presented. This variety which originated as a mutation from C. arabica L. var. typica Cramer, in 1870, in the State of Baía in North Brazil, represents a gigas form of that variety, having larger leaves, flowers and fruits, its plants being also taller; it is also known for its low productivity. Since 1933 a genetical analysis of this variety was undertaken, many of its plants being selfed and crossed with other maragogipe plants and also with individuals of the typica and bourbon varieties of C. arabica; two generations have been studied, including F2's and several back-crosses. It was concluded

  10. Mortalidade de Coccus viridis (Hemiptera: Coccidae por Lecanicillium spp. em diferentes órgãos de Coffea arabica

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    Flávio Lemes Fernandes

    2009-04-01

    Full Text Available Objetivou-se neste trabalho avaliar a mortalidade de Coccus viridis por Lecanicillium spp. em folhas e ramos de Coffea arabica. O trabalho foi realizado na casa de vegetação na Universidade Federal de Viçosa, entre fevereiro de 2005 a junho de 2006. Foram utilizadas sementes da variedade Catuaí vermelho de café (C. arabica. A solução nutritiva utilizada foi composta por 3,0 mmol/L de N, 9,0 mmol/L de K, 1,0 mmol/L de P, 4,0 mmol/L de Ca, 2,1 mmol/L de S, 46 µmol/L de B, 0,3 µmol/L de Cu, 60,0 µmol/L de Fe, 2,0 µmol/L de Mg, 0,5 µmol/L de Mo e 36,0 µmol/L de Mn. As cochonilhas e o fungo foram coletados de folhas de café em lavouras comerciais de Viçosa, MG e foram mantidos em gaiolas em Casa de Vegetação. As plantas foram infestadas com oito meses de idade. A infestação foi realizada por contato de C. viridis infectadas com o fungo sobre as cochonilhas sadias. As densidades de C. viridis viva e morta nas folhas e nos ramos foram avaliadas aos 0, 15, 30, 35, 45, 60 e 75 dias após a infestação. Usou-se análise de variância, teste de média de Skott-Knott e análise de regressão linear simples a p<0,05. O fungo Lecanicillium spp. causou mortalidade em todos os estádios de C. viridis. De forma geral, a maior mortalidade ocorreu no terceiro ínstar. Além disso, o fungo causou maior mortalidade nesse inseto praga na face abaxial da folha de café. Mortality of Coccus viridis (Hemiptera: Coccidae by Lecanicillium spp. in differents Coffea arabica organs in greenhouseAbstract. The objective of this work was to evaluate the mortality of Coccus viridis by Lecanicillium spp. on faces of the leaves, and branches of Coffea arabica. This work was conducted in greenhouse at UFV. The experiment was carried out from February 2005 to June 2006. Seeds of the lineage IAC 15 from the variety “Catuaí Vermelho” coffee (C. arabica were used. The nutritive solution utilized was composed for 3. 0 mmol/L of N, 9. 0 mmol/L of K, 1. 0 mmol

  11. Relationship between aluminum stress and caffeine biosynthesis in suspension cells of Coffea arabica L.

    Science.gov (United States)

    Pech-Kú, Roberto; Muñoz-Sánchez, J Armando; Monforte-González, Miriam; Vázquez-Flota, Felipe; Rodas-Junco, Beatriz A; González-Mendoza, Víctor M; Hernández-Sotomayor, S M Teresa

    2018-04-01

    Toxicity by aluminum is a growth-limiting factor in plants cultivated in acidic soils. This metal also promotes signal transduction pathways leading to the biosynthesis of defense compounds, including secondary metabolites. In this study, we observed that Coffea arabica L. cells that were kept in the dark did not produce detectable levels of caffeine. However, irradiation with light and supplementation of the culture medium with theobromine were the best conditions for cell maintenance to investigate the role of aluminum in caffeine biosynthesis. The addition of theobromine to the cells did not cause any changes to cell growth and was useful for the bioconversion of theobromine to caffeine. During a short-term AlCl 3 -treatment (500μM) of C. arabica cells kept under light irradiation, increases in the caffeine levels in samples that were recovered from both the cells and culture media were evident. This augmentation coincided with increases in the enzyme activity of caffeine synthase (CS) and the transcript level of the gene encoding this enzyme (CS). Together, these results suggest that actions by Al and theobromine on the same pathway lead to the induction of caffeine biosynthesis. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Alterações morfofisiológicas em folhas de Coffea arabica L. cv. "Oeiras" sob influência do sombreamento por Acacia mangium Willd Morphophysiological alterations in leaves of Coffea arabica L. cv. 'Oeiras' shaded by Acacia mangium Willd

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    Inês Angélica Cordeiro Gomes

    2008-02-01

    Full Text Available Diferenças na disponibilidade de radiação podem causar modificações na estrutura e função das folhas do cafeeiro, que podem responder de maneira diferencial à radiação por alterações morfológicas, anatômicas, de crescimento e na taxa fotossintética. O objetivo deste trabalho foi avaliar características morfofisiológicas de cafeeiros (Coffea arabica L. cv. "Oeiras" sombreados por acácia (Acacia mangium Willd. na época seca e chuvosa no sul de Minas Gerais. As maiores taxas fotossintéticas e maiores espessuras da epiderme adaxial foram observadas na estação chuvosa nas linhas de cafeeiros a pleno sol. O sombreamento influenciou em menor espessura das folhas e em espaços intercelulares maiores no tecido esponjoso. Foi também verificada mudança na forma dos cloroplastos, os quais apresentaram-se mais alongados em folhas de cafeeiros a pleno sol quando relacionados aos arborizados.Light availability is one of the most important environmental factors affecting leaf structure and functions in coffee plants that can respond differently to radiation by changes in leaf anatomy, morphology, growth and photosynthetic rate. The objective of this research was evaluate some morphophysiological aspects in leaves of coffee (Coffea arabica L. cv. 'Oeiras' cropped under shelter trees in the south of Minas Gerais during the rainy and dry season. The shade caused lower leaves thickness and higher intercellular spaces in spongious tissue. There was also verified a change in chloroplast shape, which showed more elongated in coffee tree kept at full sunlight in relation to that ones maintained on shading.

  13. Effect of roasting degree on the antioxidant activity of different Arabica coffee quality classes.

    Science.gov (United States)

    Odžaković, Božana; Džinić, Natalija; Kukrić, Zoran; Grujić, Slavica

    2016-01-01

    Coffee is one of the most widely consumed beverages in the world, because of its unique sensory properties and physiological properties. Coffee beverages represent a significant source of antioxidants in the consumers' diet and contribute significantly to their daily intake. The aim of this research was to investigate the effect of different roasting degrees on the content of biologically active compounds and antioxidant activity in different quality classes of Arabica coffee. Samples of green Arabica coffee (Rio Minas) of two quality classes from two production batches were used for the research. Roasting was carried out at temperatures of 167, 175 and 171°C. The total phenol content (TPC), total flavonoid content (TFC), flavonol content (FC) and antioxidant activity (DPPH, ABTS) in the coffee extracts was determined. This research shows that TPC was significantly higher (P coffee compared to TPC in roasted coffee, and TPC decreases as the roasting temperature increases. TFC and FC were significantly lower (P coffee than in roasted coffee. Differences in TPC between the 1st and 2nd classes of Arabica coffee were not significant (P > 0.05), while differences in TFC were significant (P coffee from the second production batch and differences in FC were significant (P coffee and for coffee roasted at 175°C. Roasting temperatures have different influences the antioxidant activity (DPPH, ABTS) of coffee and the highest antioxidant activity was determined in coffee roasted at 171°C. An exception was 1st class Arabica coffee roasted at 167°C (ABTS). All samples of 1st class Arabica coffee had higher antioxidant activity (DPPH, ABTS) compared to 2nd class Arabica. This research shows that the bioactive compounds content and antioxidant activity of different quality classes of Arabica coffee are dependent on the degree of roasting. TPC decreases when the roasting temperature increases, while TFC and FC also increase. These results indicate that the antioxidant activity

  14. Absorção, translocação e utilização de zinco, cobre e manganês por mudas enxertadas de Coffea arabica Absorption, translocation and utilization of zinc, copper and manganese in grafted seedlings of Coffea arabica

    Directory of Open Access Journals (Sweden)

    Marcelo Antonio Tomaz

    2006-04-01

    Full Text Available Comparativamente ao pé-franco, a combinação enxerto/porta-enxerto altera os complexos mecanismos de "feedback" entre parte aérea e raízes, afetando de maneira positiva ou negativa a eficiência nutricional da planta. Este trabalho teve como objetivo avaliar, em cultivo hidropônico, a eficiência da absorção, translocação e utilização de Zn, Cu e Mn por mudas de Coffea arabica L., de acordo com o porta-enxerto utilizado. O experimento foi realizado em casa de vegetação, por um período de 170 dias, em vasos que continham areia como substrato, recebendo solução nutritiva circulante. Utilizaram-se, como enxerto, quatro genótipos de C. arabica: os cultivares Catuaí Vermelho IAC 15 e Oeiras MG 6851 e os híbridos 'H 419-10-3-1-5' e 'H 514-5-5-3' , e, como porta-enxerto, quatro genótipos, sendo três de Coffea canephora Pierre ex Froenher: Apoatã LC 2258, Conilon Muriaé-1 e RC EMCAPA 8141 (recombinação entre clones da variedade Robustão Capixaba - EMCAPA 8141 e uma linhagem de Coffea arabica L.: Mundo Novo IAC 376-4, além de quatro pés-francos. O delineamento experimental utilizado foi em blocos casualizados com 20 tratamentos, quatro repetições e uma planta por parcela. A eficiência nutricional das mudas quanto ao Zn, Cu e Mn variou de acordo com a combinação enxerto/porta-enxerto. A progênie 'H 514-5-5-3' foi mais eficiente quanto à utilização de Zn, Cu e Mn e produção de matéria seca, quando combinada com os porta-enxertos Apoatã LC 2258 e Mundo Novo IAC 376-4. O Catuaí Vermelho IAC 15 foi mais eficiente na utilização de Cu e Mn quando combinado com Apoatã LC 2258.There are complexes feedback mechanisms involving the relationship rootstock/scion, whick can positively or negatively affect the plant nutrient efficiency use. The goal of the present work was to evaluate the efficiency of uptake, translocation and utilization of Zn, Cu and Mn in coffee seedlings. The experiment was carried out in a greenhouse

  15. Influence of the genotype and density of inoculation on the differentiation of somatic embryos of Coffea arabica L. cv. Red Caturra and Coffea canephora cv. Robusta

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    Raúl Barbón

    2003-07-01

    Full Text Available The conditions were established for the differentiation of somatic embryos from cell suspensions in the genotype Caturra rojo (Coffea arabica and Robusta (Coffea canephora. Cell suspensions with high embryogenic potentials and stable coefficients of multiplication were used. While studying the density of inoculation, for the phase of differentiation for both varieties, differences appeared in the embryogenic capacity among them, being reached a whole of 556 500 ES.l-1 for the variety Caturra rojo and 298 670 SE.l-1 for the variety Robusta. The biggest number of embryos in torpedo state, were obtained with a density of inoculation of 0.5 gFW.l-1 for the variety Caturra rojo and 5.0 gMF.l-1 for the variety Robusta. Key Words: cell suspensions, embryogenic potential, somatic Embryogenesis, embryogenic cells

  16. Physical, Chemicals and Flavors of Some Varieties of Arabica Coffee

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    Yusianto .

    2014-08-01

    Full Text Available Export of Arabica coffee was 28,100 tons/year or 8.28% total export of Indonesian coffee, most of them are specialty coffee. Beside their origin, variety and determine the of physical, chemical and flavors characters. The promising clones or varieties i.e. BP 416A, BP 418A, BP 430A, BP 431A, BP 432A, BP 507A, BP 508A, BP 509A, BP 511A, BP 513A, BP 516A, BP 517A and BP 518A still not be determined their quality This research was conducted to analyze their physicals, chemicals and flavors during 2 periods of harvesting (2004 and 2005, using AS 1, S 795 and USDA 762 as the control. Mature coffee berry was harvested, sorted manually, and depulped, cleaned manually and then fermented in plastic sacks during 36 hours. The fermented parchment was washed, and then sun dried, dehulled to get green coffee. Observations wre conducted on green coffee yield, husk content, color of green coffee, distribution of size, bulk density of green and roasted coffee, roasting characters, color of roasted beans, and pH, acidity and flavors. The results showed (a The lowest content of husk was BP 432A and the highest was USDA 762. The control varieties of AS 1, S 795 and USDA 762, showed husk content >15%, while those potential varieties were < 15% except BP 416A. (b Beans size >6,5 mm and more than 80% were BP 416A, BP 430A, BP 432A, BP 509A, P 88 and S 795. Green coffee of BP 430A, BP 432A and BP 509A were uniform, but S 795 was not uniform. AS 1 and BP 416A and P 88 was one group; S 795 was one group with BP 542A; BP 509 was a group with BP 432A; but BP4 30A and USDA 762 were the other groups. (c Green coffee of USDA 762 was the palest color, but BP 542A was the darkest color. AS 1 and S 795 were a group with all potential varieties, except BP 542A. (d Roasted coffee of USDA 762 was the palest color and AS 1 was the darkest. In this case, AS 1 was a group with BP 430A, BP 509A and P 88, while S 795 was a group with BP 416A and BP 432A, but USDA 762 and BP 542A were

  17. Effect of Superheated Steam Roasting on Radical Scavenging Activity and Phenolic Content of Robusta Coffee Beans

    OpenAIRE

    Ooi Ee Shan; Wahidu Zzaman; Tajul A. Yang

    2015-01-01

    Robusta coffee is one of the coffee species grown in Malaysia. However, there is little research conducted on Robusta coffee beans as Arabica coffee is more popular among the consumers. Coffee is a rich source of antioxidants, therefore research on antioxidant properties of Robusta coffee beans is important to explore its market value. Nowadays, most of coffee analysis is on conventional roasted coffee which reduces their antioxidant properties. In this study, Robusta coffee beans (Coffea can...

  18. Arabica Coffee Farming and Marketing Chain Analysis in Manggarai and EastManggarai Districts

    Directory of Open Access Journals (Sweden)

    Dhiany. Faila Sophia Hartatri

    2011-05-01

    Full Text Available Arabica coffee has a unique flavour and very potential market. The purpose of this study was to analyse Arabica coffee farming and to investigate its performance of marketing chains in Manggarai and East Manggarai Districts, Flores, East Nusa Tenggara Province. This research was conducted in 2008-2010 by interviewing coffee farmers and coffee buyers; using open and close questions. The number of respondents were 100 people in each district. The result showed that land holding per household farmer in Manggarai and East Manggarai were 0.84 ha and 0.92 ha, respectively. Farmers in both districts were within the range of productive age, the farmers who were members of farmer groups in both study sites was £ 50%. Arabica coffee cultivation was still done in a traditional way. Fertilizing and controlling of pest and diseases had not been carried out inten sively. Arabica coffee farming in both district was feasible. BCR, NPV and IRR values in Manggarai were 4.2, Rp8,530,105, and 70.76% respectively, while BCR, NPV, and IRR value in East Manggarai district were 8.1, Rp2,465,833, and 27%, respectively. BEP production and coffee price in Manggarai were 94.2 kg/ha/th and Rp15,913/kg respectively, whereas BEP production and coffee price in East Manggarai were 78,2 kg/ha/th and Rp10,134/kg, respectively. In general, farmers sold their coffee in green bean form. In general, the marketing chains of Arabica coffee in both districts was farmer – collector - trader - exporter.Key words: Arabica coffee, potential market, farming analysis, feasible, marketing chains.

  19. GIS-based multi-criteria analysis for Arabica coffee expansion in Rwanda.

    Directory of Open Access Journals (Sweden)

    Innocent Nzeyimana

    Full Text Available The Government of Rwanda is implementing policies to increase the area of Arabica coffee production. Information on the suitable areas for sustainably growing Arabica coffee is still scarce. This study aimed to analyze suitable areas for Arabica coffee production. We analyzed the spatial distribution of actual and potential production zones for Arabica coffee, their productivity levels and predicted potential yields. We used a geographic information system (GIS for a weighted overlay analysis to assess the major production zones of Arabica coffee and their qualitative productivity indices. Actual coffee yields were measured in the field and were used to assess potential productivity zones and yields using ordinary kriging with ArcGIS software. The production of coffee covers about 32 000 ha, or 2.3% of all cultivated land in the country. The major zones of production are the Kivu Lake Borders, Central Plateau, Eastern Plateau, and Mayaga agro-ecological zones, where coffee is mainly cultivated on moderate slopes. In the highlands, coffee is grown on steep slopes that can exceed 55%. About 21% percent of the country has a moderate yield potential, ranging between 1.0 and 1.6 t coffee ha-1, and 70% has a low yield potential (<1.0 t coffee ha-1. Only 9% of the country has a high yield potential of 1.6-2.4 t coffee ha-1. Those areas are found near Lake Kivu where the dominant soil Orders are Inceptisols and Ultisols. Moderate yield potential is found in the Birunga (volcano, Congo-Nile watershed Divide, Impala and Imbo zones. Low-yield regions (<1 t ha-1 occur in the eastern semi-dry lowlands, Central Plateau, Eastern Plateau, Buberuka Highlands, and Mayaga zones. The weighted overlay analysis and ordinary kriging indicated a large spatial variability of potential productivity indices. Increasing the area and productivity of coffee in Rwanda thus has considerable potential.

  20. Reducing the acidity of Arabica coffee beans by ohmic fermentation technology

    OpenAIRE

    Reta; Mursalim; Salengke; Junaedi, M.; Mariati; Sopade, P.

    2017-01-01

    Coffee is widely consumed not only because of its typical taste, but coffee has antioxidant properties because of its polygons, and it stimulates brain performance. The main problem with the consumption of coffee is its content of caffeine. Caffeine when consumed in excess, can increase muscle tension, stimulate the heart, and increase the secretion of gastric acid. In this research, we applied ohmic fermentation technology, which is specially designed to mimic the stomach. Arabica coffee has...

  1. Socioeconomic and Ecological Dimension of Certified and Conventional Arabica Coffee Production in North Sumatra, Indonesia

    OpenAIRE

    Saragih, Jef Rudiantho

    2013-01-01

    The study was conducted in six subdistricts of Simalungun district, North Sumatra, Indonesia. The research objective is knowing the influence of socioeconomic and ecological factors on production of specialty Arabica coffee. Determination of the households sample was using Probability Proportional to Size and Simple Random Sampling for 79 units certified coffee farms and 210 units conventional coffee farms. Farmer’s data was analyzed with multiple linear regression model. Benefit of coffee ce...

  2. Floral Stimulation and Behavior of Insect Pollinators Affected by Pyraclostrobin on Arabica Coffee

    OpenAIRE

    Tarno, Hagus; Wicaksono, Karuniawan Puji; Begliomini, Edson

    2018-01-01

    Coffee is the most valuable traded commodity after oil. On coffee, bees act to support a pollination that is shown by the number of harvested berries. This research aimed to evaluate the use of pyraclostrobin on flowering stage and insect pollinators on Arabica Coffee. Experiment was conducted in Kalisat Coffee Farm, Jampit, Bondowoso, ca. 1600 meters after sea level from October 2013 to April 2014. Randomized Block Design was adopted in this experiment. Three doses of pyraclostrobin and cont...

  3. Eventos do processo de infecção de Colletotrichum gloeosporioides inoculados em folhas de Coffea arabica L

    Directory of Open Access Journals (Sweden)

    Josimar Batista Ferreira

    2009-12-01

    Full Text Available O presente trabalho teve por objetivo obter informações sobre os eventos de pré-penetração, penetração e colonização de isolados de C. gloeosporioides, obtidos de mangueira e cafeeiro, quando inoculados em folhas de cafeeiros (Coffea arabica L. da cultivar Catucaí Vermelho. As folhas foram selecionadas, padronizadas e lavadas, demarcando-se áreas circulares de 0,5 cm de diâmetro na face abaxial, inoculando-se uma alíquota de 20 µL da suspensão de conídios. Utilizou-se um isolado obtido de mangueira e dois isolados obtidos de cafeeiro com mancha manteigosa. Realizaram-se avaliações com 3, 6, 8, 12, 24, 36, 48, 72, 96, 144 e 240 horas após a inoculação (hai. Todos os materiais foram processados e observados em microscópico eletrônico de varredura. Os conídios de todos os isolados aderiram freqüentemente nas depressões das células da epiderme e células-guarda dos estômatos, formando septo antes da germinação. A penetração, na maior parte, se deu por via direta e algumas vezes por estômatos. Isolados de cafeeiro germinaram em folhas de 6 a 8 hai, produzindo apressórios 12 hai e acérvulos de 96 a 144 hai. O isolado de mangueira germinou de 6 a 8 hai com formação de apressório de 8 a 12 hai e produziu novos conídios diretamente em hifas conidiogênicas. Não foi observada a formação acérvulos para este isolado.The present study was carried out with the objective to provide information on the pre-penetration, penetration and colonization events of isolates of Colletotrichum gloeosporioides from mango and coffee, when inoculated in coffee leaves. The cultivar Catucaí Vermelho was used in all experiments. Coffee leaves were selected, standardized and washed in sterile water. After that, circular areas of 0.5 cm in diameter were marked on the undersurface of the leaves. In the center of each circular area, 20 µL of spore suspension was deposited. One isolate from mango and two from coffee presenting blister

  4. Fumonisin B2 production by Aspergillus niger in Thai coffee beans

    NARCIS (Netherlands)

    Noonim, P.; Mahakarnchanakul, W.; Nielsen, K.F.; Frisvad, J.C.; Samson, R.A.

    2009-01-01

    During 2006 and 2007, a total of 64 Thai dried coffee bean samples (Coffea arabica) from two growing sites in Chiangmai Province and 32 Thai dried coffee bean samples (Coffea canephora) from two growing sites in Chumporn Province, Thailand, were collected and assessed for fumonisin contamination by

  5. Synthèse des résultats de recherche sur l'agronomie du caféier arabica (Coffea arabica L.) au Rwanda au 31 mars 1994

    OpenAIRE

    Rutunga, V.; Kavamahangat, F.; Nsengimana, C.

    1999-01-01

    Synthesis of Results on Coffee Agronomy Research in Rwanda on 31 March 1994. Arabica coffee plays an important role in the macro-economic performance of Rwanda. As a result, Agricultural Research Institutes have done research on coffee agronomy, aimed at improving coffee productivite The ecological conditions for coffee in Rwanda are characterized by inadequate rainfall and high light intensity. Soil parental material and chemical properties are variable amongst different small holders coffee...

  6. Interferência de plantas daninhas sobre o crescimento inicial de Coffea arabica Weed effect on the initial growth of Coffea arabica

    Directory of Open Access Journals (Sweden)

    C.M.T. Fialho

    2011-03-01

    Full Text Available Objetivou-se com este trabalho avaliar os efeitos da interferência de plantas daninhas, em diferentes densidades de infestação, sobre o crescimento de plantas jovens de café arábica. Mudas de café, cultivar Mundo Novo, foram transplantadas, no estádio de quatro a cinco pares de folhas completamente expandidas, para vasos com capacidade de 25 dm³. O experimento foi conduzido em casa de vegetação em blocos casualizados, em esquema fatorial (4 x 4; o primeiro fator foi composto por espécies de plantas daninhas (Digitaria horizontalis, Brachiaria decumbens, Brachiaria plantaginea e Mucuna aterrima, e o segundo, por densidades dessas espécies (zero, dois, quatro e seis plantas por vaso, com quatro repetições. O plantio das espécies daninhas foi realizado 60 dias após o transplantio do café (0 DAT. Nesta data e no dia do encerramento do experimento, aos 90 DAT, determinaram-se a altura, a área foliar e o diâmetro do caule da planta de café, para cálculo do incremento dessas variáveis. Aos 90 DAT, determinou-se a matéria seca da parte aérea e radicular do café e das plantas daninhas e a densidade radicular do café. Utilizando esses resultados, estimou-se a razão de massa foliar, razão de massa caulinar, razão de massa radicular, razão de área foliar e a razão sistema radicular/parte aérea das plantas de café. A espécie M. aterrima foi a que mais reduziu a taxa de crescimento, a área foliar, a matéria seca do caule e das folhas e o diâmetro do caule do café. Entre as gramíneas, B. plantaginea foi a que mais reduziu a taxa de crescimento, área foliar, diâmetro do caule e densidade radicular do café. Ocorreu relação negativa entre a densidade de plantas daninhas e as variáveis de crescimento e com a razão de massa radicular e razão sistema radicular/parte aérea.The goal of this work was to evaluate the effects of weeds on the growth of young Arabica coffee plants, at different densities of infestation

  7. A liquid chromatography/electrospray ionisation tandem mass spectrometry method for the simultaneous quantification of salicylic, jasmonic and abscisic acids in Coffea arabica leaves.

    Science.gov (United States)

    de Sá, Marta; Ferreira, João P; Queiroz, Vagner T; Vilas-Boas, Luís; Silva, Maria C; Almeida, Maria H; Guerra-Guimarães, Leonor; Bronze, Maria R

    2014-02-01

    Plants have developed an efficient system of recognition that induces a complex network of signalling molecules such as salicylic acid (SA), jasmonic acid (JA) and abscisic acid (ABA) in case of a pathogenic infection. The use of specific and sensitive methods is mandatory for the analysis of compounds in these complex samples. In this study a liquid chromatography/electrospray ionisation tandem mass spectrometry method was developed and validated for the simultaneous quantification of SA, JA and ABA in Coffea arabica (L.) leaves in order to understand the role of these phytohormones in the signalling network involved in the coffee defence response against Hemileia vastatrix. The results showed that the method was specific, linear (r ≥ 0.99) in the range 0.125-1.00 µg mL⁻¹ for JA and ABA and 0.125-5.00 µg mL⁻¹ for SA, and precise (relative standard deviation ≤11%), and the limit of detection (0.010 µg g⁻¹ fresh weight) was adequate for quantifying these phytohormones in this type of matrix. In comparison with healthy leaves, those infected with H. vastatrix (resistance reaction) displayed an increase in SA level 24 h after inoculation, suggesting the involvement of an SA-dependent pathway in coffee resistance. © 2013 Society of Chemical Industry.

  8. Influence of the in vitro environment on the germination of somatic embryos of Coffea arabica L. cv. 'Caturra rojo' and Clematis tangutica K.

    Directory of Open Access Journals (Sweden)

    Raúl Barbon

    2017-07-01

    Full Text Available The in vitro environment is a factor that in recent years has begun to investigate, because gases such as oxygen, carbon dioxide and ethylene play an important role in the morphogenesis of somatic embryos and their development in plants. The objective of this work was to determine the effect of the CO2 on the germination of coffee somatic embryos (Coffea arabica L. cv. 'Caturra rojo' and clematis (Clematis tangutica K.. Three gas mixtures composed of CO2 concentrations (2.5, 5.0 and 10.0% combined with 21% O2 and two controls (passive exchange and forced ventilation were used. A positive effect of CO2 on the germination of somatic embryos in the torpedo stage in coffee and clematis was obtained, because in the treatments with passive exchange, where there was CO2 accumulation, germination of the somatic embryos was superior to the treatments with Forced ventilation. With 2.5% and 5.0% CO2, the germination process is stimulated while with 10.0% CO2 there is an inhibition of germination with the appearance of malformations and hyperhydricity.   Keywords: gaseous atmosphere, carbon dioxide, somatic embryogenesis, secondary embryogenesis, hyperhydricity

  9. The Greater Phenotypic Homeostasis of the Allopolyploid Coffea arabica Improved the Transcriptional Homeostasis Over that of Both Diploid Parents.

    Science.gov (United States)

    Bertrand, Benoît; Bardil, Amélie; Baraille, Hélène; Dussert, Stéphane; Doulbeau, Sylvie; Dubois, Emeric; Severac, Dany; Dereeper, Alexis; Etienne, Hervé

    2015-10-01

    Polyploidy impacts the diversity of plant species, giving rise to novel phenotypes and leading to ecological diversification. In order to observe adaptive and evolutionary capacities of polyploids, we compared the growth, primary metabolism and transcriptomic expression level in the leaves of the newly formed allotetraploid Coffea arabica species compared with its two diploid parental species (Coffea eugenioides and Coffea canephora), exposed to four thermal regimes (TRs; 18-14, 23-19, 28-24 and 33-29°C). The growth rate of the allopolyploid C. arabica was similar to that of C. canephora under the hottest TR and that of C. eugenioides under the coldest TR. For metabolite contents measured at the hottest TR, the allopolyploid showed similar behavior to C. canephora, the parent which tolerates higher growth temperatures in the natural environment. However, at the coldest TR, the allopolyploid displayed higher sucrose, raffinose and ABA contents than those of its two parents and similar linolenic acid leaf composition and Chl content to those of C. eugenioides. At the gene expression level, few differences between the allopolyploid and its parents were observed for studied genes linked to photosynthesis, respiration and the circadian clock, whereas genes linked to redox activity showed a greater capacity of the allopolyploid for homeostasis. Finally, we found that the overall transcriptional response to TRs of the allopolyploid was more homeostatic compared with its parents. This better transcriptional homeostasis of the allopolyploid C. arabica afforded a greater phenotypic homeostasis when faced with environments that are unsuited to the diploid parental species. © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  10. Observações citológicas em Coffea: XV - Microsporogênese em Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    A. J. T. Mendes

    1950-03-01

    Full Text Available No presente trabalho são apresentadas as observações realizadas sôbre a microsporogênese nas variedades semperflorens e caturra, de Coffea arabica L. Notou-se que, no início da prófase, os cromossômios se colorem muito mal, não permitindo observações sôbre a sua morfologia; em paquitene, os cromossômios se apresentam com várias secções heteropicnóticas separadas por secções muito finas, que se colorem mal; o centrômero é bastante nítido e se acha ladeado de zonas bem heteropicnóticas; as extremidades dos braços dos cromossômios se colorem mal e se perdem no meio do citoplasma ; o nucléolo é bastante visível e a êle se acham ligados alguns cromossômios. É difícil determinar o número exato de cromossômios ligados ao nucléolo, tendo-se encontrado de 1 a 4. De paquitene a metáfase I, as fases se sucedem rapidamente. Em diplotene, os cromossômios são curtos, não mais se percebendo o centrômero. Em diaquinese os 22 pares de cromossômios se repelem pela sua parte mais colorida, onde se encontra o centrômero, e se unem pela parte clara, onde se notam os quiasmas ; o número de quiasmas, por célula, varia de 29 a 43 ; a média por bivalente é de 1,67, em semperjlorens, e 1,75, em caturra. Em metáfase I, o número médio de quiasmas, por bivalente,. é de 1,69, em semperjlorens, e 1,67, em caturra. Em anáfase I, os 22 pares de cromossômios se separam normalmente. Em telófase I, os cromossômios se colorem mal. Não há, praticamente, intercinese; os cromossômios contraem-se de novo e entram em anáfase II. A formação dos micrósporos é normal. Depois de soltos, ocorre a divisão nuclear, dando origem a dois núcleos com 22 cromossômios. Isto ocorre três a quatro dias antes da abertura das flores; o núcleo vegetativo é grande, esférico e homogêneo, colo-rindo-se mal; o núcleo reprodutivo é menor, reticulado, colore-se bem e se localiza na periferia da célula; ao seu redor se destaca uma por

  11. Genética de coffea VI: independência dos fatores xc xc (xanthocarpa e br br (bronze em coffea arabica L.

    Directory of Open Access Journals (Sweden)

    C. A. Krug

    1942-01-01

    Full Text Available Em artigos anteriores (1, 2 os autores demonstraram que a cor amarela dos frutos e a coloração bronzeada das folhas novas são, em Coffea arabica L, controladas, cada uma, por um único par de fatores genéticos (respectivamente xc xc e Br Br. Os híbridos F1 no primeiro caso com plantas de frutos vermelhos, e no segundo com plantas de folhas novas verdes, demonstraram tratar-se de casos em que há dominância incompleta nesta geração, os frutos híbridos possuindo uma coloração vermelho clara e as folhas novas se apresentando com uma tonalidade bronze clara. Como algumas das hibridações realizadas envolviam, ao mesmo tempo, os dois caracteres em questão, apresentou-se a oportunidade para constatar se havia ou não independência entre os dois pares de fatores que controlam estes caracteres. Neste artigo apresentam-se os resultados das observações realizadas, tanto em diversas populações de F2 como também em dois back-crosses. Os dados confirmam plenamente a hipótese estabelecida, isto é, da independência entre os dois pares de fatores em questão (xc xc e Br Br. Este fato era esperado à vista do número relativamente elevado de cromosômios nas variedades cruzadas (2n = 44.In two previous publications (1,2 the authors demonstrated that in Coffea arabica L. yellow fruit color and bronze color of young leaves are each controled by one pair of genes (respectively xc xc and Br Br. The F1 hybrids, in the first instance between plants with yellow and red fruits and in the second instance between plants with bronze and green colored young leaves, showed incomplete dominance of both characters, the F1 fruits being of a light red color and the F1 young leaves of a light bronze one. As some of the crosses involved both pairs of genes, it was possible to find out wether they are linked or independent. In the present article the authors are publishing the results obtained with several F2 populations and also with 2 types of back crosses

  12. Population dynamics and distribution of the coffee berry borer ...

    African Journals Online (AJOL)

    Population dynamics and distribution of coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae) were studied on Coffea arabica L. in southwestern region of Ethiopia. Thirty coffee trees were sampled at weekly intervals from 2000 to 2001. Findings of this study showed that coffee berry borer population ...

  13. Conversion of nicotinic acid to trigonelline is catalyzed by N-methyltransferase belonged to motif B′ methyltransferase family in Coffea arabica

    International Nuclear Information System (INIS)

    Mizuno, Kouichi; Matsuzaki, Masahiro; Kanazawa, Shiho; Tokiwano, Tetsuo; Yoshizawa, Yuko; Kato, Misako

    2014-01-01

    Graphical abstract: Trigonelline synthase catalyzes the conversion of nicotinic acid to trigonelline. We isolated and characterized trigonelline synthase gene(s) from Coffea arabica. - Highlights: • Trigonelline is a major compound in coffee been same as caffeine is. • We isolated and characterized trigonelline synthase gene. • Coffee trigonelline synthases are highly homologous with coffee caffeine synthases. • This study contributes the fully understanding of pyridine alkaloid metabolism. - Abstract: Trigonelline (N-methylnicotinate), a member of the pyridine alkaloids, accumulates in coffee beans along with caffeine. The biosynthetic pathway of trigonelline is not fully elucidated. While it is quite likely that the production of trigonelline from nicotinate is catalyzed by N-methyltransferase, as is caffeine synthase (CS), the enzyme(s) and gene(s) involved in N-methylation have not yet been characterized. It should be noted that, similar to caffeine, trigonelline accumulation is initiated during the development of coffee fruits. Interestingly, the expression profiles for two genes homologous to caffeine synthases were similar to the accumulation profile of trigonelline. We presumed that these two CS-homologous genes encoded trigonelline synthases. These genes were then expressed in Escherichiacoli, and the resulting recombinant enzymes that were obtained were characterized. Consequently, using the N-methyltransferase assay with S-adenosyl[methyl- 14 C]methionine, it was confirmed that these recombinant enzymes catalyzed the conversion of nicotinate to trigonelline, coffee trigonelline synthases (termed CTgS1 and CTgS2) were highly identical (over 95% identity) to each other. The sequence homology between the CTgSs and coffee CCS1 was 82%. The pH-dependent activity curve of CTgS1 and CTgS2 revealed optimum activity at pH 7.5. Nicotinate was the specific methyl acceptor for CTgSs, and no activity was detected with any other nicotinate derivatives, or with

  14. Conversion of nicotinic acid to trigonelline is catalyzed by N-methyltransferase belonged to motif B′ methyltransferase family in Coffea arabica

    Energy Technology Data Exchange (ETDEWEB)

    Mizuno, Kouichi, E-mail: koumno@akita-pu.ac.jp [Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, Akita 010-0195 (Japan); Matsuzaki, Masahiro [Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, Akita 010-0195 (Japan); Kanazawa, Shiho [Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, Bunkyo-ku, Tokyo 112-8610 (Japan); Tokiwano, Tetsuo; Yoshizawa, Yuko [Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, Akita 010-0195 (Japan); Kato, Misako [Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, Bunkyo-ku, Tokyo 112-8610 (Japan)

    2014-10-03

    Graphical abstract: Trigonelline synthase catalyzes the conversion of nicotinic acid to trigonelline. We isolated and characterized trigonelline synthase gene(s) from Coffea arabica. - Highlights: • Trigonelline is a major compound in coffee been same as caffeine is. • We isolated and characterized trigonelline synthase gene. • Coffee trigonelline synthases are highly homologous with coffee caffeine synthases. • This study contributes the fully understanding of pyridine alkaloid metabolism. - Abstract: Trigonelline (N-methylnicotinate), a member of the pyridine alkaloids, accumulates in coffee beans along with caffeine. The biosynthetic pathway of trigonelline is not fully elucidated. While it is quite likely that the production of trigonelline from nicotinate is catalyzed by N-methyltransferase, as is caffeine synthase (CS), the enzyme(s) and gene(s) involved in N-methylation have not yet been characterized. It should be noted that, similar to caffeine, trigonelline accumulation is initiated during the development of coffee fruits. Interestingly, the expression profiles for two genes homologous to caffeine synthases were similar to the accumulation profile of trigonelline. We presumed that these two CS-homologous genes encoded trigonelline synthases. These genes were then expressed in Escherichiacoli, and the resulting recombinant enzymes that were obtained were characterized. Consequently, using the N-methyltransferase assay with S-adenosyl[methyl-{sup 14}C]methionine, it was confirmed that these recombinant enzymes catalyzed the conversion of nicotinate to trigonelline, coffee trigonelline synthases (termed CTgS1 and CTgS2) were highly identical (over 95% identity) to each other. The sequence homology between the CTgSs and coffee CCS1 was 82%. The pH-dependent activity curve of CTgS1 and CTgS2 revealed optimum activity at pH 7.5. Nicotinate was the specific methyl acceptor for CTgSs, and no activity was detected with any other nicotinate derivatives, or

  15. Rapid approach to identify the presence of Arabica and Robusta species in coffee using 1H NMR spectroscopy.

    Science.gov (United States)

    Monakhova, Yulia B; Ruge, Winfried; Kuballa, Thomas; Ilse, Maren; Winkelmann, Ole; Diehl, Bernd; Thomas, Freddy; Lachenmeier, Dirk W

    2015-09-01

    NMR spectroscopy was used to verify the presence of Arabica and Robusta species in coffee. Lipophilic extracts of authentic roasted and green coffees showed the presence of established markers for Robusta (16-O-methylcafestol (16-OMC)) and for Arabica (kahweol). The integration of the 16-OMC signal (δ 3.165 ppm) was used to estimate the amount of Robusta in coffee blends with an approximate limit of detection of 1-3%. The method was successfully applied for the analysis of 77 commercial coffee samples (coffee pods, coffee capsules, and coffee beans). Furthermore, principal component analysis (PCA) was applied to the spectra of lipophilic and aqueous extracts of 20 monovarietal authentic samples. Clusters of the two species were observed. NMR spectroscopy can be used as a rapid prescreening tool to discriminate Arabica and Robusta coffee species before the confirmation applying the official method. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. Levantamento dos riscos existentes à segurança e à saúde do trabalhador na pós-colheita de café (Coffea arabica conforme as exigências das normas regulamentadoras Survey of the existing risks to the security and health of the worker in the after harvesting of the coffee plant (Coffea arabica by according to the requirements of regulatory norms

    Directory of Open Access Journals (Sweden)

    Cinara da Cunha Siqueira Carvalho

    2008-04-01

    Full Text Available A segurança no trabalho é um tema que vem chamando muito a atenção do setor agrícola, devido à sua importância como um dos itens considerados para a aquisição da certificação, que agrega valor ao café e conseqüentemente, melhora o preço para o produtor. Considerando a existência dos riscos em todas as atividades e a falta de percepção por parte do empregador e do trabalhador no processo de pós-colheita do café, bem como a falta de conformidade das propriedades quanto à aplicação das normas regulamentadoras do Ministério do Trabalho e do Emprego (M.T.E, o objetivo deste trabalho foi diagnosticar os riscos envolvidos em cada uma das etapas da pós-colheita do café e sua percepção por parte dos trabalhadores e proprietários. Além disso, precisamos verificar se unidades de processamento de café estão em conformidade com as normas regulamentoras, a fim de determinar os pontos de risco que levem os órgãos governamentais a auxiliar os produtores na modificação do processo para possibilitar a exportação do café. O trabalho foi realizado a partir da aplicação de questionário em trinta propriedades localizadas em três associações rurais do município de São Sebastião do Paraíso - MG, definidas por sorteio. A análise dos questionários mostrou que, somente 23% das pessoas entrevistadas possuíam percepção dos riscos existentes nas propriedades e somente 3% delas estavam dentro dos padrões de conformidade com as NR's. Esses dados estão relacionados ao baixo nível tecnológico, à falta de treinamento dos funcionários e às dificuldades de acesso à informação por parte dos produtores.The security in the work is a subject that has calling the attention of the agricultural sector, due to its importance as one of itens considered for the acquisition of the certification, which aggregates value to the coffee plant and consequently improves the price for the producer. Considering the existence of the risks

  17. Physical and Flavor Quality of Some Potential Varieties of Arabica Coffee in Several Interval Storage Periods

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    Yusianto .

    2006-12-01

    Full Text Available Coffee storage was an active process, where the quality and flavor was depend on the origin, humidity, temperature, period, and ware house condition. The objective of this research was to know quality and flavor of some Arabica coffee varieties in interval of storage periods. The examined coffee varieties were BP 416 A, BP 430 A, BP 432 A, BP 509 A, BP 542 A, P 88, AS 1, S 795, and USDA-762. The treatments were recent harvest, one and two years stored green coffee. The green coffee were wet processed, sun dried, packed in polyethylene bags, one kg/pack and placed in some covered plastic boxes. The boxes were stored in ware house covered with wavy asbes roof and flat asbes ceiling. The green coffee was examined for its moisture content, color, and bulk density. The green coffee was roasted at medium level, and then examined for its the bulk density, yield, volume of swelling, and color of the roasted and powdered. The flavors examination was blind test method. The research showed that storage period significantly influenced the moisture content, color, and bulk density of green coffee, yield, volume of swelling, color of roasted coffee, color, and flavor profile of coffee powder. Those varieties tested showed significantly different on the moisture content, green coffee color, roasted coffee color, coffee powder color, and the profile flavor. The storage period influenced the green coffee color from greenish-gray to yellowish-red. The bulk density of green coffee decreased. The varieties that showed a little color changeduring storage, were BP 430 A,BP 416 A, AS 1, and S 795. One year of storage periode, the green coffee was still had the original color, but after two years, the original color had changed totally. The powder of recent harvest coffee was darker than that of one and two years storage. One year stored coffee had higher quality of aroma, intensity of aroma, quality of flavor, intensity of flavor, acidity, quality of after taste

  18. Recent Advances in the Genetic Transformation of Coffee

    Science.gov (United States)

    Mishra, M. K.; Slater, A.

    2012-01-01

    Coffee is one of the most important plantation crops, grown in about 80 countries across the world. The genus Coffea comprises approximately 100 species of which only two species, that is, Coffea arabica (commonly known as arabica coffee) and Coffea canephora (known as robusta coffee), are commercially cultivated. Genetic improvement of coffee through traditional breeding is slow due to the perennial nature of the plant. Genetic transformation has tremendous potential in developing improved coffee varieties with desired agronomic traits, which are otherwise difficult to achieve through traditional breeding. During the last twenty years, significant progress has been made in coffee biotechnology, particularly in the area of transgenic technology. This paper provides a detailed account of the advances made in the genetic transformation of coffee and their potential applications. PMID:22970380

  19. Arbuscular mycorrhiza alters metal uptake and the physiological response of Coffea arabica seedlings to increasing Zn and Cu concentrations in soil.

    Science.gov (United States)

    Andrade, S A L; Silveira, A P D; Mazzafera, P

    2010-10-15

    Studies on mycorrhizal symbiosis effects on metal accumulation and plant tolerance are not common in perennial crops under metal stress. The objective of this study was to evaluate the influence of mycorrhization on coffee seedlings under Cu and Zn stress. Copper (Cu) and zinc (Zn) uptake and some biochemical and physiological traits were studied in thirty-week old Coffea arabica seedlings, in response to the inoculation with arbuscular mycorrhizal fungi (AMF) and to increasing concentrations of Cu or Zn in soil. The experiments were conducted under greenhouse conditions in a 2×4 factorial design (inoculation or not with AMF and 0, 50, 150 and 450mgkg(-1) Cu or 0, 100, 300 and 900mgkg(-1) Zn). Non-mycorrhizal plants maintained a hampered and slow growth even in a soil with appropriate phosphorus (P) levels for this crop. As metal levels increased in soil, a greater proportion of the total absorbed metals were retained by roots. Foliar Cu concentrations increased only in non-mycorrhizal plants, reaching a maximum concentration of 30mgkg(-1) at the highest Cu in soil. Mycorrhization prevented the accumulation of Cu in leaves, and mycorrhizal plants showed higher Cu contents in stems, which indicated a differential Cu distribution in AMF-associated or non-associated plants. Zn distribution and concentrations in different plant organs followed a similar pattern independently of mycorrhization. In mycorrhizal plants, only the highest metal concentrations caused a reduction in biomass, leading to significant changes in some biochemical indicators, such as malondialdehyde, proline and amino acid contents in leaves and also in foliar free amino acid composition. Marked differences in these physiological traits were also found due to mycorrhization. In conclusion, AMF protected coffee seedlings against metal toxicity. Copyright © 2010 Elsevier B.V. All rights reserved.

  20. Effect of 6-BA on nodal explant bud sproutings of Coffea arabica cv. Mundo Novo Efeito de 6-BA na brotação de gemas de explantes nodais de Coffea arabica cv. Mundo Novo

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    Luis Carlos da Silva Ramos

    2005-01-01

    Full Text Available Coffee plants can be micropropagated by nodal bud sprouting using the 6-benzylaminopurine (6-BA hormone. However, literature reports the use of a wide range of 6-BA, from 0.5 to 88.8 µM L-1. So, this study was performed to narrow that range. Nodal explants of Coffea arabica cv Mundo Novo obtained from in vitro plantlets were inoculated on gelled-MS medium supplemented with different concentrations of 6-BA. Two assays were carried out: in the first one, 6-BA was used at concentrations of 0, 5, 25, 50, and 100 µM L-1, being evaluated at 43 and 123 days. In the second experiment, dosis of 10, 20 and 30 µM L-1, have evaluated at 65 and 100 days. Treatments with 6-BA induced multiple sprouting from the nodal explants, which were best characterized around 100 days after inoculation. The nodal explants grew taller and showed multiple shoots, whereas the effect of 6-BA at 5 to 25 µM L-1 was similar to that with higher concentrations (50 and 100 µM L-1. Nodal explants yielded from 2.9 to 6.0 buds per node, achieving height of 1.3 to 1.5 cm at 5 to 25 µM L-1 of 6-BA, whereas they yielded from 4.3 to 4.9 buds per node but the sprouting grew about 0.8 cm at 50 and 100 µM L-1 of 6-BA. This study indicated that multiple sprouting of lateral buds can be induced by lower concentrations of 6-BA, for example, from 10 to 30 µM L-1, diminishing possible risks of somaclonal variation due to high levels of hormone concentration.O cafeeiro pode ser micropropagado via brotação de gemas laterais, aplicando o regulador de crescimento 6-benzilaminopurina (6-BA. Entretanto, a literatura apresenta ampla variação da dose empregada, desde 0.5 a 88.8 µM L-1. Assim, este estudo visou otimizar doses para explantes nodais do cafeeiro C. arabica cv Mundo Novo. Explantes nodais, obtidos de plântulas cultivadas in vitro, foram inoculados em meio MS geleificado, com adição de diferentes concentrações de 6-BA. Foram feitos dois experimentos: no primeiro, 6-BA foi

  1. Qualidade da bebida em espécies e populações derivadas de híbridos interespecíficos de Coffea Cupping quality of coffee species and interspecific hybrids

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    Alcides Carvalho

    1990-01-01

    Full Text Available Avaliou-se, em dois experimentos, a qualidade da bebida das espécies Coffea canephora e C. congensis e derivados de híbridações interespecíficas [C. canephora duplicado (dp x C. arabica, C. canephora x C. eugenioides, C. arabica x C. dewevrei dp e C. racemosa x C. arabica]. Por tratar-se de análise de bebida de cafés pouco conhecidos, avaliou-se a eficiência de uma escala de 1 a 10 pontos em comparação à escala de 0 a 5 pontos utilizada para C. arabica. Foram, também, acrescentadas pelos provadores indicações relacionadas ao gosto da bebida. A escala de 6 pontos mostrou-se pouco eficaz na discriminação dos tratamentos e a de 10 pontos, utilizada alternativamente, revelou-se mais eficiente nos dois experimentos. No primeiro, verificou-se, quanto à qualidade, uma superioridade dos grupos C arabica x C. dewevrei dp e C. canephora dp x C. arabica pelas duas escalas. No segundo experimento, o grupo C. racemosa x C. arabica apresentou a maior média, apesar de não diferir, pela escala 1, dos demais grupos. Pela escala 2, superou, no entanto, os grupos C. canephora e C. congensis. Gostos incomuns foram observados nas amostras com relação à bebida. Atribuem-se à grande diversidade do material analisado e a falta de familiarização dos provadores com espécies bem diferentes de C. arabica, certas discrepâncias com relação à determinação desses defeitos nas amostras analisadas.Investigations on coffee cupping quality of the species C. canephora, C. congensis and several populations derived from interespecific crosses (C. canephora dp x C. arabica, C. canephora x C. eugenioides, C. arabica x C. dewevrei dp and C. racemosa x C. arabica were undertaken in two trials, in order to guide breeding programs using this germoplasm. Two grading systems were used for classification of the cupping quality, the normal six points scale used for C. arabica samples and a new one with 10 points, 1 for the lowest and 10 for the highest quality

  2. Avaliação de genótipos de cafeeiros Arabica e Robusta no estado do Acre Evaluation of Arabica and Robusta coffee genotypes in the state of Acre

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    Celso Luis Bergo

    2008-02-01

    Full Text Available Conduziu-se este trabalho, com o objetivo de introduzir e avaliar 40 genótipos de cafeeiros das espécies Coffea arabica e Coffea canephora nas condições edafoclimáticas do Estado do Acre, visando disponibilizar aos cafeicultores acreanos, cultivares com melhor potencial produtivo. Da espécie C. arabica foram avaliados genótipos das cultivares Icatu, Bourbon, Mundo Novo, Catuaí, Obatã e Catimor. Da espécie C. canephora foram avaliadas as cultivares Conilon e Robusta, caracterizadas como Grupo Robusta. Os genótipos utilizados foram provenientes do Instituto Agronômico de Campinas (IAC e da Embrapa Rondônia. O experimento foi conduzido no Campo Experimental da Embrapa Acre, Rio Branco, AC, no período de 1995 a 2004. O delineamento experimental foi em blocos casualizados com cinco repetições. As características avaliadas foram: produtividade, altura, diâmetro da copa e vigor. Da espécie C. arabica, grupo Icatu, destacou-se Icatu-PR-182039-1(IAC H 4782-7-788 com produtividade média de café beneficiado de 34 sc/ ha, Icatu IAC-4041; Icatu IAC-2945; Icatu IAC-2944-MT; Icatu IAC-4040 e Icatu IAC-4046 com produtividade variando de 20 a 26 sacas. Para o grupo Catuaí os melhores genótipos foram Obatã IAC 4275, Obatã IAC 1169 e Catimor IAC 4466 com produtividade média de café beneficiado de 49, 45 e 37 sacas por hectare respectivamente. Na espécie C. canephora foram avaliados 8 genótipos das cultivares Conilon e Robusta e quanto à produtividade não houve diferença estatística, observou-se incremento de 7 sacas/ha para a variedade Conilon IAC 66-3 quando comparado ao Conilon plantado na região. Nesta espécie os genótipos apresentaram sintomas de deficiência hídrica na época seca (julho/agosto.This work aimed to introduce and evaluate 40 coffee genotypes of Coffea arabica e Coffea canephora species in soil and weather conditions of Acre and had as main goal to provide cultivars to coffee producers with better potential of

  3. A Real Option Analysis applied to the production of Arabica and Robusta Coffee in Ecuador

    Energy Technology Data Exchange (ETDEWEB)

    Jácome, A.R.; Garrido, A.

    2017-09-01

    The coffee market is distinguished for being volatile and uncertain in terms of domestic and international prices. Arabica and Robusta coffee are produced in 23 provinces of Ecuador. A decade-long decline of coffee production prompted the Ecuadorian government to launch a public program for replanting coffee trees towards the end of 2011. A grower’s decision to enter, remain in or exit the coffee sector is based on fluctuating profits from each year’s harvest sale. We analyzed the hypothesis whereby the coffee grower’s decision to leave the sector is explained by volatile and uncertain prices. This paper aimed to evaluate the coffee sector with an application of Real Option Analysis for the period 2002-2012. We also defined entry (H) and exit (L) prices for Arabica and Robusta coffee for the analyzed period. Our findings revealed high H and L prices encourage growers to leave the sector for the most part of the analyzed period. High H and L prices resulted from high variable cost due to increasing wages for farm workers. The Ecuadorian government is developing a policy to help growers make production more efficient, encouraging them to remain in the sector in the long run.

  4. A Real Option Analysis applied to the production of Arabica and Robusta Coffee in Ecuador

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    Andres R. Jácome

    2017-04-01

    Full Text Available The coffee market is distinguished for being volatile and uncertain in terms of domestic and international prices. Arabica and Robusta coffee are produced in 23 provinces of Ecuador. A decade-long decline of coffee production prompted the Ecuadorian government to launch a public program for replanting coffee trees towards the end of 2011. A grower’s decision to enter, remain in or exit the coffee sector is based on fluctuating profits from each year’s harvest sale. We analyzed the hypothesis whereby the coffee grower’s decision to leave the sector is explained by volatile and uncertain prices. This paper aimed to evaluate the coffee sector with an application of Real Option Analysis for the period 2002-2012. We also defined entry (H and exit (L prices for Arabica and Robusta coffee for the analyzed period. Our findings revealed high H and L prices encourage growers to leave the sector for the most part of the analyzed period. High H and L prices resulted from high variable cost due to increasing wages for farm workers. The Ecuadorian government is developing a policy to help growers make production more efficient, encouraging them to remain in the sector in the long run.

  5. A Real Option Analysis applied to the production of Arabica and Robusta Coffee in Ecuador

    International Nuclear Information System (INIS)

    Jácome, A.R.; Garrido, A.

    2017-01-01

    The coffee market is distinguished for being volatile and uncertain in terms of domestic and international prices. Arabica and Robusta coffee are produced in 23 provinces of Ecuador. A decade-long decline of coffee production prompted the Ecuadorian government to launch a public program for replanting coffee trees towards the end of 2011. A grower’s decision to enter, remain in or exit the coffee sector is based on fluctuating profits from each year’s harvest sale. We analyzed the hypothesis whereby the coffee grower’s decision to leave the sector is explained by volatile and uncertain prices. This paper aimed to evaluate the coffee sector with an application of Real Option Analysis for the period 2002-2012. We also defined entry (H) and exit (L) prices for Arabica and Robusta coffee for the analyzed period. Our findings revealed high H and L prices encourage growers to leave the sector for the most part of the analyzed period. High H and L prices resulted from high variable cost due to increasing wages for farm workers. The Ecuadorian government is developing a policy to help growers make production more efficient, encouraging them to remain in the sector in the long run.

  6. Differences in arbuscular mycorrhizal fungi among three coffee cultivars in Puerto Rico

    Science.gov (United States)

    Ligia Lebrón; Jean D. Lodge; Paul. Bayman

    2012-01-01

    Mycorrhizal symbiosis is important for growth of coffee (Coffea arabica), but differences among coffee cultivars in response to mycorrhizal interactions have not been studied. We compared arbuscular mycorrhizal (AM) extraradical hyphae in the soil and diversity of AM fungi among three coffee cultivars, Caturra, Pacas, and Borbon, at three farms in...

  7. EVALUACIÓN DE CUATRO ESPECIES FORESTALES ASOCIADAS CON CAFÉ (Coffea Arabica L. Y EN MONOCULTIVO EN EL LITORAL ECUATORIANO

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    Pedro Suatunce Cunuhay

    2009-12-01

    Full Text Available Se implantó un ensayo de sistema agroforestal con café (Coffea arabica L. en el área del cantón Quevedo, en la Finca Experimental “La Represa”, en el año de 1997. Se plantaron cuatro especies forestales en asociación con café (C. arabica L., y también se establecieron parcelas de árboles y café en monocultivo, en parcelas contiguas. Las especies forestales utilizadas fueron el guayacán blanco (Cybistax donnell-smithii Rose, laurel prieto (Cordia megalantha Chadat, fernansánchez (Triplaris cumingiana Wedd y teca (Tectona grandis L. F.. El objetivo fue comparar la producción de madera y café bajo los sistemas agroforestales y en los sistemas de plantación en monocultivo tanto de los árboles como del café. La producción por hectárea de café fue mayor en monocultivo. La incidencia (% del minador del café (Perileucoptera coffeella Green fue baja en los dos sistemas, además no hubo diferencias significativas entre los cafetales asociados y en monocultivo. El volumen de las especies forestales fue mayor en los sistemas agroforestales. Según la evaluación del uso eficiente de la tierra, cualquiera de los sistemas agroforestales evaluados es mejor que los cultivos puros de estas especies.

  8. Interações entre auxinas e ácido bórico, no enraizamento de estacas caulinares de Coffea arabica L. cv. Mundo Novo Interactions between auxins and boric acid in the rooting of stem cuttings Coffea arabica L. cv. "Mundo Novo"

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    E.O. Ono

    1992-01-01

    Full Text Available O presente trabalho teve como finalidade, estudar o efeito de auxinas e do boro no enraizamento de estacas caulinares de Coffea arabica L. cv. "Mundo Novo". As estacas foram retiradas de ramos ortotrópicos semi-lenhosos de cafeeiro, as quais foram tratadas durante 24 horas com soluções de IBA ou NAA e boro, e a mistura das três substâncias, resultando um total de 14 tratamentos. Para a avaliação do objetivo em questão, foram realizadas as seguintes observações, mediante coleta após 90 dias de plantio: número de estacas enraizadas e número de estacas com calos. Através dos resultados obtidos, pode-se concluir que, para obter um maior número de estacas enraizadas, é conveniente o tratamento com NAA à 100 ou 200 ppm mais boro.The present research had as purpose to study auxin and boron effects on rooting of Coffea arábica L. cv. "Mundo Novo" stem cuttings. The cuttings were taken from orthotropous semi-hardwood branches of coffee-tree, which were treated during 24 hours with IBA or NAA and boron solutions, and the mixture of the three substances, resulting a total of 14 treatments. The following observations were realized, taking the cuttings 90 days after planting: number of rooted cuttings and "callus" formation per cutting. It can be concluded that to obtain a higher number of rooted cuttings, the treatment with NAA at 100 or 200 ppm plus boron is the most suitable.

  9. Selected parameters of arabica coffee quality affected by its geographical origin

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    Alica Bobková

    2017-01-01

    Full Text Available The aim of this paper was to evaluate selected parameters of Arabica coffee quality. Arabica coffee beans originated from 21 different regions of the world. Parameters of their moisture content, water extract, water extract in dry matter, dry mater, caffeine and caffeine content in dry matter were assessed by the Slovak Technical Standard. Dry matter content ranged from 98.64 to 99.07%, the highest content was measured in sample from Cuba. Minimum dry matter content was detected in coffee beans from Mexico. Caffeine in studied samples ranged from 10 200 mg.kg-1 to 13 500 mg.kg-1. The lowest caffeine content was determined in Panama coffee, the highest was found in the sample from Indonesia. The results of moisture content and caffeine in dry mater were evaluated by the Food Code of the Slovak Republic and all observed parameters in the coffee beans meet the maximum levels given in legislation. By statistical procesing it can be seen that coffee samples originating from Ecuador, Indonesia and Nepal were similar in parameters of caffeine content and caffeine in dry matter. Other similar samples originating from Cuba, Peru, Ethiopia and Panama were statistically similar at dry matter content. Special statistical group was coffee from Salvador at the parameters of water extract and water extract in dry matter. Normal 0 21 false false false EN-GB X-NONE X-NONE

  10. Impacts of leaf age and heat stress duration on photosynthetic gas exchange and foliar nonstructural carbohydrates in Coffea arabica.

    Science.gov (United States)

    Marias, Danielle E; Meinzer, Frederick C; Still, Christopher

    2017-02-01

    Given future climate predictions of increased temperature, and frequency and intensity of heat waves in the tropics, suitable habitat to grow ecologically, economically, and socially valuable Coffea arabica is severely threatened. We investigated how leaf age and heat stress duration impact recovery from heat stress in C. arabica . Treated plants were heated in a growth chamber at 49°C for 45 or 90 min. Physiological recovery was monitored in situ using gas exchange, chlorophyll fluorescence (the ratio of variable to maximum fluorescence, F V / F M ), and leaf nonstructural carbohydrate (NSC) on mature and expanding leaves before and 2, 15, 25, and 50 days after treatment. Regardless of leaf age, the 90-min treatment resulted in greater F V / F M reduction 2 days after treatment and slower recovery than the 45-min treatment. In both treatments, photosynthesis of expanding leaves recovered more slowly than in mature leaves. Stomatal conductance ( g s ) decreased in expanding leaves but did not change in mature leaves. These responses led to reduced intrinsic water-use efficiency with increasing heat stress duration in both age classes. Based on a leaf energy balance model, aftereffects of heat stress would be exacerbated by increases in leaf temperature at low g s under full sunlight where C. arabica is often grown, but also under partial sunlight. Starch and total NSC content of the 45-min group significantly decreased 2 days after treatment and then accumulated 15 and 25 days after treatment coinciding with recovery of photosynthesis and F V / F M . In contrast, sucrose of the 90-min group accumulated at day 2 suggesting that phloem transport was inhibited. Both treatment group responses contrasted with control plant total NSC and starch, which declined with time associated with subsequent flower and fruit production. No treated plants produced flowers or fruits, suggesting that short duration heat stress can lead to crop failure.

  11. Coffee berry disease in Kenya

    NARCIS (Netherlands)

    Vermeulen, H.

    1979-01-01

    Data are presented on research in Kenya in 1964 - 1969 on anatomical, mycological, epidemiological, chemical control and cultural aspects of coffee berry disease, Colletotrichum coffeanum Noack, of Coffea arabica L. The pathogen causes flower and berry

  12. Reducing the acidity of Arabica coffee beans by ohmic fermentation technology

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    Reta

    2017-07-01

    Full Text Available Coffee is widely consumed not only because of its typical taste, but coffee has antioxidant properties because of its polygons, and it stimulates brain performance. The main problem with the consumption of coffee is its content of caffeine. Caffeine when consumed in excess, can increase muscle tension, stimulate the heart, and increase the secretion of gastric acid. In this research, we applied ohmic fermentation technology, which is specially designed to mimic the stomach. Arabica coffee has high acidity that needs to be reduced than Luwak coffee, although it is cheaper. Hence, the ohmic technology with a time and temperature variation were applied to measure the total acidity of the coffee to determine optimum fermentation conditions. Results revealed that the total acidity of the coffee varied with fermentation conditions (0.18% – 0.73%. Generally, the longer the fermentation and the higher the temperature, the lower the total acidity. The acidity of the Luwak coffee through natural fermentation was 2.34%, which is substantially higher than the total acidity from the ohmic samples. Ohmic-based fermentation technology, therefore, offers improvements in coffee quality.

  13. Characterisation of Arabica Coffee Pulp - Hay from Kintamani - Bali as Prospective Biogas Feedstocks

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    Hendroko Setyobudi Roy

    2018-01-01

    Full Text Available The huge amount of coffee pulp waste is an environmental problem. Anaerobic fermentation is one of the alternative solutions. However, availability of coffee pulp does not appear for year-round, whereas biogas needs continuous feedstocks for digester stability. This research uses coffee pulp from Arabica Coffee Factory at Mengani, Kintamani, Bali–Indonesia. The coffee pulp was transformed into coffee pulp-hay product by sun drying for preservations to extend the raw materials through the year. Characterization of coffee pulp-hay was conducted after to keep for 15 mo for review the prospect as biogas feedstocks. Several parameters were analyzed such as C/N ratio, volatile solids, carbohydrate, protein, fat, lignocellulose content, macro-micro nutrients, and density. The review results indicated that coffee pulp-hay is prospective raw material for biogas feedstock. This well-proven preservation technology was able to fulfill the continuous supply. Furthermore, some problems were found in the recent preliminary experiment related to the density and fungi growth in the conventional laboratory digester. Further investigation was needed to implement the coffee pulp – hay as biogas feedstocks.

  14. Respuesta fotosintética de Coffea arabica L. a diferentes niveles de luz y disponibilidad hídrica

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    Yesid Alejandro Mariño

    2014-04-01

    Full Text Available El sombrío permite una mejor aclimatación del café (Coffea arabica L. en zonas donde el invierno se caracteriza por bajas temperaturas nocturnas seguido de días soleados y el verano, por altas temperaturas y lluvias ocasionales. En este estudio, realizado en Viçosa-MG, Brasil, se analizaron los efectos de dos condiciones de luminosidad (pleno sol y 15% de la radiación solar y disponibilidades de agua en el suelo sobre las relaciones hídricas y el desempeño fotosintético en C. arabica en dos épocas contrastantes. Independientemente de la época de evaluación, las plantas cultivadas en condiciones de baja disponibilidad hídrica presentaron reducciones en la fotosíntesis (A, conductancia estomática (g s y en la razón Ci/Ca en los dos tratamientos lumínicos. Estos cambios fueron acompañados con la reducción en la conductancia hidráulica y la transpiración. No se presentaron diferencias significativas en la producción cuántica efectiva, en el coeficiente de extinción no-fotoquímica y en la productividad cuántica basal de los procesos no fotoquímicos. Sobre la disponibilidad hídrica adecuada, las plantas cultivadas al sol presentaron reducciones en A, g s y Ci/Ca en la época de invierno. Adicionalmente, se encontraron limitaciones fotoquímicas con la reducción en la razón de las fluorescencias variable e inicial. Los resultados indican que cualquier recomendación de uso de sombrío como práctica de manejo debe ser tomada con precaución.

  15. Resistência de plantas de coffea arabica, coffea canephora e híbridos interespecíficos à cercosporiose

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    Flávia Rodrigues Alves Patricio

    2010-12-01

    Full Text Available O presente estudo objetivou avaliar a resistência de plantas de Coffea arabica, C. canephora e de híbridos interespecíficos, à cercosporiose (Cercospora coffeicola, importante doença da cultura do cafeeiro. Foram realizados dois experimentos em 2003, em Campinas (SP, com mudas das cultivares de C. arabica, Mundo Novo IAC 388-17-1, Mundo Novo IAC 376-4, Bourbon Amarelo, Bourbon Vermelho, Obatã IAC 1669-20, Catuaí Vermelho IAC 144, Catuaí Amarelo IAC 62, Icatu Precoce IAC 3282, Icatu Vermelho IAC 4045, Ouro Verde IAC H5010-5 e Tupi IAC 1669-33; de C. canephora, Robusta IAC 1653-7 e Apoatã IAC 2258, além de dois híbridos interespecíficos Piatã IAC 387 e Híbrido de Timor IAC 1559-13. As mudas foram submetidas à inoculação com uma suspensão de conídios preparada com cinco isolados coletados em diferentes regiões do Estado de São Paulo. Os materiais mais suscetíveis foram Robusta IAC 1653-7, Apoatã IAC 2258, Híbrido de Timor IAC 1559-13, Bourbon Vermelho e Bourbon Amarelo com incidência entre 63,1% e 81,6% no primeiro experimento e entre 70% e 93,3% no segundo e severidade (notas 1-6 entre 2,92 e 3,42 no primeiro experimento e entre 3,64 e 2,79 no segundo. Os materiais mais resistentes foram Piatã IAC 387, Ouro Verde IAC H5010-5 e Tupi IAC 1669-33, com incidência entre 28,4% e 35,5% no primeiro experimento e 21,1% e 55,6% no segundo, e severidade entre 1,44 e 1,73 e entre 1,28 e 1,88, respectivamente, no primeiro e segundo experimentos.

  16. EFEITO DE Ilex paraguariensis St. Hil. e Coffea arabica L. SOBRE O CRESCIMENTO DE Fonsecaea pedrosoi ATCC 46428

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    Maria Lucia Scroferneker

    2007-12-01

    Full Text Available Este trabalho avaliou o efeito de extratos aquosos de Ilex paraguariensis (erva-mate e Coffea arabica (café em ágar Sabouraud dextrose no crescimento de Fonsecaea pedrosoi ATCC 46428. F. pedrosoi foi cultivada em placas de Petri contendo ágar Sabouraud dextrose suplementado com extratos aquosos derivados de 0,5; 1; 2; 3; 4 e 5g de pó de erva-mate ou de café fervidos em 100ml de água destilada por 30 min. Os diâmetros das colônias do fungo foram determinados após 7 dias. A incorporação dos extratos de erva-mate ou café no meio de crescimento não causou diferenças significativas no crescimento radial de F. pedrosoi ATCC 46428 comparado ao controle. Entretanto, estudos sobre o requerimento nutricional são importantes na sistematização do perfil bioquímico, o que pode contribuir na elucidação da bioquímica funcional do microrganismo.

  17. Observações anatômicas em plantas de Coffea arabica L. obtidas por enraizamento de estacas

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    Adriana Madeira Santos Jesus

    2010-04-01

    Full Text Available Uma forma para se obter diminuição significativa de tempo e recursos despendidos nos programas de melhoramento de Coffea arabica L. é a clonagem de híbridos F1 por meio de estacas caulinares. Alguns estudos, em diferentes instituições, foram realizados buscando-se definir um método eficiente para esse tipo de clonagem. Com o objetivo de verificar-se a presença de barreiras anatômicas ao enraizamento de estacas caulinares do cafeeiro e a origem das raízes adventícias, bem como compara-las às raízes provenientes de plantas obtidas por semeadura, foram realizadas análises anatômicas no Departamento de Biologia da Universidade Federal de Lavras (UFLA, Lavras, MG. Utilizaram-se estacas caulinares de cafeeiro dos cultivares Acaiá e Rubi e mudas obtidas por semeadura direta e por estaquia. Os cortes realizados nas estacas caulinares mostraram não existirem barreiras anatômicas ao enraizamento adventício. Nas estacas enraizadas, a origem do primórdio radicular foi próxima aos tecidos vasculares. Cortes histológicos nas raízes formadas nas estacas e nas raízes de mudas obtidas por semeadura confirmaram que elas apresentam as mesmas estruturas primárias.

  18. Estudio preliminar de la comunidad de hongos endófitos en hojas de café (Coffea arabica

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    Mónica Milena Morales Valencia

    2006-01-01

    Full Text Available Los endófitos son organismos que viven asintomáticamente dentro de tejidos vegetales vivos. Este grupo no está restringido a los hongos sino que también se pueden encontrar bacterias e incluso invertebrados. Hasta el momento han sido poco estudiados, sobre todo en zonas tropicales. En el presente trabajo se hizo un estudio preliminar de la diversidad de la comunidad de hongos endófitos presentes en hojas de café (Coffea arabica en tres sitios del departamento de Cundinamarca, Colombia. Se muestrearon fragmentos foliares que representaron un área total de 2.700 mm2, procedentes de 15 árboles, en los cuales se encontraron 279 individuos de 38 morfotipos diferentes. El taxón más abundante fue Colletotrichum, el cual estuvo representado por el 47% de todos los individuos encontrados. Las comunidades de hongos endófitos estudiadas se ajustaron al modelo de distribución logarítmica normal, es decir, muchas especies raras y pocas abundantes.

  19. Genética de Coffea. XVIII - Variegação no cafeeiro Leaf variegation in coffee plants

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    A. Carvalho

    1954-01-01

    Full Text Available A variegação das fôlhas do cafeeiro tanto em plantas novas como em plantas adultas tem sido freqüentemente observada. Plantas variegadas podem ocorrer espontaneamente nas progénies das diversas variedades de Coffea arabica L., motivo porque não devem constituir variedade distinta dessa espécie, como foi proposto por Cramer. As plantas variegadas em estudo puderam ser agrupadas em duas categorias, isto é, aquelas em que a variegação não se acha associada com anomalias na forma e textura das folhas e aquelas nas quais a variegação é acompanhada dessas anomalias. No primeiro grupo o padrão de variegação não é uniforme para tôdas as fôlhas, enquanto no segundo grupo o padrão é semelhante para tôda a planta. Usualmente apenas alguns ramos das plantas do primeiro grupo são variegados. Observou-se algumas vezes que as duas fôlhas do mesmo verticilo têm padrões semelhantes de variegação. Encontraram-se também fôlhas variegadas nas quais a metade da lâmina é variegada ou mesmo albina, enquanto a outra metade apresenta a côr verde normal. Os dados genéticos obtidos, embora preliminares para a maioria das plantas variegadas em investigação, permitiram concluir que o padrão de variegação da planta 180, pertencente ao primeiro grupo, é herdado pelo citoplasma e não é transmitido pelo pólen. O padrão de variegação da planta 253-21, pertencente ao segundo grupo, no entanto, parece ser transmitido pelo polen.Leaf variegation in coffee plants is a relatively common abnormality, and variegated seedlings have been found to occur spontaneously in progenies of most varieties of Coffea arabica L. For this reason it is thought that variegated types should not be described as distinct varieties, as it was done before by Cramer. Variegated coffee plants can be grouped in two categories : first, those in which variegated leaves are normal in shape and texture, but do not show a uniform variegation pattern; and second, those

  20. Simple Sequence Repeat Analysis of Selected NSIC-registered Coffee Varieties in the Philippines

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    Daisy May C. Santos

    2016-06-01

    Full Text Available Coffee (Coffea sp. is an important commercial crop worldwide. Three species of coffee are used as beverage, namely Coffea arabica, C. canephora, and C. liberica. Coffea arabica L. is the most cultivated among the three coffee species due to its taste quality, rich aroma, and low caffeine content. Despite its inferior taste and aroma, C. canephora Pierre ex A. Froehner, which has the highest caffeine content, is the second most widely cultivated because of its resistance to coffee diseases. On the other hand, C. liberica W.Bull ex Hierncomes is characterized by its very strong taste and flavor. The Philippines used to be a leading exporter of coffee until coffee rust destroyed the farms in Batangas, home of the famous Kapeng Barako. The country has been attempting to revive the coffee industry by focusing on the production of specialty coffee with registered varieties on the National Seed Industry Council (NSIC. Correct identification and isolation of pure coffee beans are the main factors that determine coffee’s market value. Local farms usually misidentify and mix coffee beans of different varieties, leading to the depreciation of their value. This study used simple sequence repeat (SSR markers to evaluate and distinguish Philippine NSIC-registered coffee species and varieties. The neighbor-joining tree generated using PAUP showed high bootstrap support, separating C. arabica, C. canephora, and C. liberica from each other. Among the twenty primer pairs used, seven were able to distinguish C. arabica, nine for C. liberica, and one for C. canephora.

  1. Extraction and evaluation of bioactive compounds with antioxidant potential from green arabica coffee extract

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    Simona PATRICHE

    2015-12-01

    Full Text Available During the last decade researches concerning the essential role of coffee in health and disease prevention showed an increased development. In the present study we obtained extracts from three green Arabica coffee varieties which demonstrated a significant antioxidant potential due to the presence in their composition of two bioactive compounds, caffeine and chlorogenic acids. The content and antioxidant activity of bioactive compounds were evaluated by qualitative and quantitative analyses using spectrophotometric and chromatography methods. The chlorogenic acid was found in high concentrations, being followed by gallic, p-coumaric and ferulic acids. The highest caffeine contents were found in the green coffee extracts of the Supremo–Columbia and Top Quality–Kenya products.

  2. A Preliminary Study on Rainfall Interception Loss and Water Yield Analysis on Arabica Coffee Plants in Central Aceh Regency, Indonesia

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    Reza Benara

    2012-12-01

    Full Text Available Rainfall interception loss from plants or trees can reduce a net rainfall as source of water yield. The amount of rainfall interception loss depends on kinds of plants and hydro-meteorological characteristics. Therefore, it is important to study rainfall interception loss such as from Arabica Coffee plantation which is as main agricultural commodity for Central Aceh Regency. In this study, rainfall interception loss from Arabica Coffee plants was studied in Kebet Village of Central Aceh Regency, Indonesia from January 20 to March 9, 2011. Arabica coffee plants used in this study was 15 years old, height of 1.5 m and canopy of 4.567 m2. Rainfall interception loss was determined based on water balance approach of daily rainfall, throughfall, and stemflow data. Empirical regression equation between rainfall interception loss and rainfall were adopted as a model to estimate rainfall interception loss from Arabica Coffee plantation, which the coefficient of correlation, r is 0.98. In water yield analysis, this formula was applied and founded that Arabica Coffee plants intercept 76% of annual rainfall or it leaved over annual net rainfall 24% of annual rainfall. Using this net rainfall, water yield produced from Paya Bener River which is the catchment area covered by Arabica Coffee plantation was analyzed in a planning of water supply project for water needs domestic of 3 sub-districts in Central Aceh Regency. Based on increasing population until year of 2025, the results showed that the water yield will be not enough from year of 2015. However, if the catchment area is covered by forest, the water yield is still enough until year of 2025

  3. Genética de Coffea: XV - Hereditariedade dos característicos principais de Coffea arabica L. Var. semperflorens K.M.C.

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    A. Carvalho

    1952-06-01

    Full Text Available No planalto de São Paulo, o cafeeiro normalmente floresce duas a quatro vezes por ano, nos períodos compreendidos entre fins de julho a novembro. Raramente floresce mais vêzes, e um pouco além dessa estação. Em 1934, foram encontrados alguns cafeeiros da espécie C. arabica, caracterizados por seu florescimento quase que continuamente durante o ano. A êsse mutante foi dada a denominação de semperflorens. Os resultados da análise genética apresentados indicam que os característicos principais do semperflorens, a forma da planta, tipo de ramificação e florescimento quase que contínuo, são controlados por um par de fatôres genéticos recessivos. Êsse fator genético tem por símbolo sf sf, correspondente à abreviação da palavra semperflorens. Os resultados dos cruzamentos entre o semperflorens e as variedades murta e nana indicam que o semperflorens deve ter-se originado como uma mutação recessiva do bourbon. Apesar de terem sido encontrados cafeeiros semperflorens quase que simultâneamente em Ribeirão Prêto e Campinas, é mais provável que a mutação tenha ocorrido em Ribeirão Preto, onde o café bourbon foi cultivado pela primeira vez em São Paulo. Além de apresentar o semperflorens interêsse do ponto de vista fisiológico, tem também valor econômico, por ser produtivo e possuir boa resistência à sêca, motivo pelo qual numerosas progênies dêsse cafeeiro vêm sendo estudadas, visando o isolamento de linhagens ainda mais produtivas.Under normal environmental conditions coffee plants in the State of São Paulo flower two to four times during the period of July to November. Only rarely do they flower beyond these limits. In 1934 a few coffee plants of the species C. arabica were found which flower almost at any period of the year. This mutant was named semperflorens. The results of the gene tical analysis here presented indicate that the characteristics of this mutant are controled by one pair of recessive

  4. Glyphosate Accumulation and Detrimental Effects on Coffea Arabica

    DEFF Research Database (Denmark)

    Schrübbers, Lars Christoph

    and the MS/MS system provided a limit of quantification (LOQ) below 0.1 mg/kg; the commonly used maximum residue limit (MRL) for glyphosate in plant derived food products. Glyphosate was found in all samples analyzed from different coffee fields, regardless of management practices. AMPA was not detected......Coffee is one of the most popular beverages worldwide and a highly traded commodity. In order to maintain a high yield of the perennial crop, weed competition for resources needs to be reduced. For this purpose herbicides are commonly applied, with glyphosate being one of the most prominent...

  5. Levels of Antioxidant Activity and Fluoride Content in Coffee Infusions of Arabica, Robusta and Green Coffee Beans in According to their Brewing Methods.

    Science.gov (United States)

    Wolska, J; Janda, Katarzyna; Jakubczyk, K; Szymkowiak, M; Chlubek, D; Gutowska, I

    2017-10-01

    Coffee is a rich source of dietary antioxidants, and this property links with the fact that coffee is one of the world's most popular beverages. Moreover, it is a source of macro- and microelements, including fluoride. The aim of this work was to determine antioxidant activity of coffee beverages and fluoride content depending on different coffee species and conditions of brewing. Three species of coffee, arabica, robusta and green coffee beans obtained from retail stores in Szczecin (Poland) were analyzed. Five different techniques of preparing drink were used: simple infusion, french press, espresso maker, overflow espresso and Turkish coffee. Antioxidant potential of coffee beverages was investigated spectrophotometrically by DPPH method. Fluoride concentrations were measured by potentiometric method with a fluoride ion-selective electrode. Statistical analysis was performed using Stat Soft Statistica 12.5. Antioxidant activity of infusions was high (71.97-83.21% inhibition of DPPH) depending on coffee species and beverage preparing method. It has been shown that the method of brewing arabica coffee and green coffee significantly affects the antioxidant potential of infusions. The fluoride concentration in the coffee infusions changed depending, both, on the species and conditions of brewing, too (0.013-0.502 mg/L). Methods of brewing didn't make a difference to the antioxidant potential of robusta coffee, which had also the lowest level of fluoride among studied species. Except overflow espresso, the fluoride content was the highest in beverages from green coffee. The highest fluoride content was found in Turkish coffee from green coffee beans.

  6. Floral Stimulation and Behavior of Insect Pollinators Affected by Pyraclostrobin on Arabica Coffee

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    Hagus Tarno

    2018-02-01

    Full Text Available Coffee is the most valuable traded commodity after oil. On coffee, bees act to support a pollination that is shown by the number of harvested berries. This research aimed to evaluate the use of pyraclostrobin on flowering stage and insect pollinators on Arabica Coffee. Experiment was conducted in Kalisat Coffee Farm, Jampit, Bondowoso, ca. 1600 meters after sea level from October 2013 to April 2014. Randomized Block Design was adopted in this experiment. Three doses of pyraclostrobin and control were used as treatments such as 1.0, 1.5 and 2.0 cc L-1 of pyraclostrobin, and repeated three times. Percentage of fallen flower, fruiting stage, fruit production, frequency of bee`s visitation, and bee`s behavior was observed as variables in this experiment. Results showed that 1 percentage of fallen flowers was reduced by applying pyraclostrobin at 1.5 and 2.0 cc L-1 up to 50 % compared to control, 2 flowering rate was faster than control at 1.5 and 2.0 cc L-1 of pyraclostrobin, 3 application of 1.5 – 2.0 cc L-1 of pyraclostrobin increased the number of young fruits and pinheads, and 4 pollinators preferred to visit flowers of coffee trees which sprayed by pyraclostrobin than control treatment especially Apis mellifera.

  7. Personal exposure to dust and endotoxin in Robusta and Arabica coffee processing factories in Tanzania.

    Science.gov (United States)

    Sakwari, Gloria; Mamuya, Simon H D; Bråtveit, Magne; Larsson, Lennart; Pehrson, Christina; Moen, Bente E

    2013-03-01

    Endotoxin exposure associated with organic dust exposure has been studied in several industries. Coffee cherries that are dried directly after harvest may differ in dust and endotoxin emissions to those that are peeled and washed before drying. The aim of this study was to measure personal total dust and endotoxin levels and to evaluate their determinants of exposure in coffee processing factories. Using Sidekick Casella pumps at a flow rate of 2l/min, total dust levels were measured in the workers' breathing zone throughout the shift. Endotoxin was analyzed using the kinetic chromogenic Limulus amebocyte lysate assay. Separate linear mixed-effects models were used to evaluate exposure determinants for dust and endotoxin. Total dust and endotoxin exposure were significantly higher in Robusta than in Arabica coffee factories (geometric mean 3.41 mg/m(3) and 10 800 EU/m(3) versus 2.10 mg/m(3) and 1400 EU/m(3), respectively). Dry pre-processed coffee and differences in work tasks explained 30% of the total variance for total dust and 71% of the variance for endotoxin exposure. High exposure in Robusta processing is associated with the dry pre-processing method used after harvest. Dust and endotoxin exposure is high, in particular when processing dry pre-processed coffee. Minimization of dust emissions and use of efficient dust exhaust systems are important to prevent the development of respiratory system impairment in workers.

  8. In vitro AND in vivo ANTHELMINTIC EFFECT OF Coffea arabica RESIDUES AGAINTS AN Haemonchus contortus ISOLATE WITH LOW SUSCEPTIBILITY TO TANNINS

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    G.I. Ortiz-Ocampo

    2016-05-01

    Full Text Available The present study evaluated in vitro and in vivo anthelmintic (AH effect of the percolated by-product of Coffea arabica against a Haemonchus contortus isolate with low susceptibility to tannins. Firstly, the susceptibility to tannins of Paraíso H. contortus isolate was evaluated with the in vitro larval exsheathment inhibition assay (LEIA using an acetone:water (70:30 extract of Acacia pennatula. Afterwards, the in vitro AH effect of acetone:water extracts of percolated C. arabica by-products (Clean and Smooth (CS and Shade Grown (SG Starbucks® were evaluated. Then, the in vivo AH effect was determined in growing sheep (4 months old and 15.2 kg bodyweight, infected with H. contortus (Paraiso isolate and fed with a diet containing the percolated by-product of C. arabica. Three experimental groups were formed (n = 6 lambs: GC Group (control diet without C. arabica, GCA Group (feed containing 10% C. arabica and, GCA+PEG (polyethilenglycol Group (feed with 10% C. arabica + PEG as a tannin inhibitor. Fecal samples were obtained daily from the rectum of lambs (days 1 to 20 of the study. The H. contortus isolate tolerated the A. pennatula extract at concentrations of 150 and 300 μg extract/ml PBS (P > 0.05, which confirmed its low susceptibility to tannins. The C. arabica CS extract reduced the exsheatment from 150 μg of extract/ml PBS and the SG variety from 1200 μg of extract/ml PBS (P0.05. In conclusion, the C. arabica percolated by-product extracts showed in vitro AH effect against exsheathment. However, the consumption of feed containing 10% of the percolated by-product did not show an in vivo AH effect in sheep infected with the Paraiso H. contortus isolate. The latter could be due to the low susceptibility towards the secondary compounds of tannin rich plants of the H. contortus isolate used in this study.

  9. Effect of greenhouse conditions on the leaf apoplastic proteome of Coffea arabica plants.

    Science.gov (United States)

    Guerra-Guimarães, Leonor; Vieira, Ana; Chaves, Inês; Pinheiro, Carla; Queiroz, Vagner; Renaut, Jenny; Ricardo, Cândido P

    2014-06-02

    This work describes the coffee leaf apoplastic proteome and its modulation by the greenhouse conditions. The apoplastic fluid (APF) was obtained by leaf vacuum infiltration, and the recovered proteins were separated by 2-DE and subsequently identified by matrix assisted laser desorption/ionization time of flight-mass spectrometry, followed by homology search in EST coffee databases. Prediction tools revealed that the majority of the 195 identified proteins are involved in cell wall metabolism and in stress/defense responses. Although most of the proteins follow the classical secretory mechanism, a low percentage of them seem to result from unconventional secretion (leaderless secreted proteins). Principal components analysis revealed that the APF samples formed two distinct groups, with the temperature amplitude mostly contributing for this separation (higher or lower than 10°C, respectively). Sixty one polypeptide spots allowed defining these two groups and 28 proteins were identified, belonging to carbohydrate metabolism, cell wall modification and proteolysis. Interestingly stress/defense proteins appeared as more abundant in Group I which is associated with a higher temperature amplitude. It seems that the proteins in the coffee leaf APF might be implicated in structural modifications in the extracellular space that are crucial for plant development/adaptation to the conditions of the prevailing environment. This is the first detailed proteomic study of the coffee leaf apoplastic fluid (APF) and of its modulation by the greenhouse conditions. The comprehensive overview of the most abundant proteins present in the extra-cellular compartment is particularly important for the understanding of coffee responses to abiotic/biotic stress. This article is part of a Special Issue entitled: Environmental and structural proteomics. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Variation of Potential Yield of Hybrid Population of Robusta coffee (Coffea canepor

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    Novie Pranata Erdiansyah

    2014-08-01

    Full Text Available The low yield of Robusta coffee in Indonesia may be due to the use of planting materials derived from seeds. The research objective was to determine the variation of Robusta coffee yield wich local propagated by using seeds. The study was conducted in Kaliwining experimental Station of ICCRI (Indonesian Coffee andCocoa Research Institute. There were two populations observed. Number of progeny used in this study were 186 genotypes consisting of two groups from crossesBP 409 x Q 121 with 89 progenies and BP 961 x BP 409 with 81 progenies. The results showed that planting materials from seeds exhibit properties mixed results.Progeny that have the best results (yield more than 2 ton/ha not more than 5% of the total population. In both populations there is a big difference between the progenythat has high and low yield. Highest yield B population could reach 2,500 kg/ha and the C population reached 2,200 kg/ha. The lowest yield can only produce coffee270 kg/ha in populations B and 120 kg/ha in population C.Key words: Coffea canephora, hybrid, variation, yield

  11. Anatomic and physiological modifications in seedlings of Coffea arabica cultivar Siriema under drought conditions

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    Emanuelle Ferreira Melo

    2014-02-01

    Full Text Available Due to the weather changes prognostic for the coming years, the understanding of water deficit and physiological responses of plants to drought becomes an important requirement in order to develop technologies such as mechanisms to assist plants to cope with longer drought periods, which will be essential to maintenance of Brazilian and worldwide production. This study aimed to evaluate ecophysiological and anatomical aspects as well as the nitrate reductase activity in Siriema coffee seedlings subjected to four treatments: Daily irrigated, non-irrigated, re-irrigated 24 hours and re-irrigated 48 hours after different stress periods. Non-irrigation promoted a reduction in leaf water potential being accented from the ninth day of evaluation onwards. Re-irrigation promoted a partial recovery of the plant water potential. Non-irrigated plants showed an increase in stomatal resistance and reduction of transpiration and nitrate reductase activity. In the roots, there was a decrease in nitrate reductase activity under water stress. Leaf anatomical modifications were significant only for the adaxial surface epidermis and palisade parenchyma thickness, this latter characteristic being higher in control plants. Stomatal density and polar and equatorial diameter ratios showed the highest values in plants under water stress. In the roots, differences only in the cortex thickness being bigger in the non-irrigated treatment could be observed. Therefore, Siriema coffee plants under water stress show physiological, biochemical and anatomical modifications that contribute to the tolerance of this genotype to these conditions.

  12. Glyphosate accumulation, translocation, and biological effects in Coffea arabica after single and multiple exposures

    DEFF Research Database (Denmark)

    Schrübbers, Lars Christoph; Valverde, Bernal E.; Strobel, Bjarne W.

    2016-01-01

    In perennial crops like coffee, glyphosate drift exposure can occur multiple times during its commercial life span. Due to limited glyphosate degradation in higher plants, a potential accumulation of glyphosate could lead to increased biological effects with increased exposure frequency....... In this study, we investigated glyphosate translocation over time, and its concentration and biological effects after single and multiple simulated spray-drift exposures. Additionally, shikimic acid/glyphosate ratios were used as biomarkers for glyphosate binding to its target enzyme.Four weeks after...... the exposure, glyphosate was continuously translocated. Shikimic acid levels were lin-ear correlated with glyphosate levels. After two months, however, glyphosate appeared to have reduced activity. In the greenhouse, multiple applications resulted in higher internal glyphosate concentrations.The time...

  13. Response of Arabica Coffee Cultivated on Andisols on Organic Matter Applications

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    Pujiyanto .

    2013-12-01

    Full Text Available Andisols  are characterized  by  dominance  of  amorphous  minerals  which form strong and stable bonding with organic matter, therefore Andisols always contain high organic matter. For that reason, organic fertilizer is generally not applied  on  Andisols,  because  it  is  assumed  that  it  will  not  give   any  positive effect  on  growth  or  yield.  The  experiment  was  aimed  to  evaluate  response  of mature Kartika 1  Arabica coffee variety (seven years old cultivated on  Andisols applied with organic matter derived from cow dung manure. The experiment was carried out at Andungsari  Experimental Station located in Bondowoso District, East  Java. Elevation of the site was 1,150 m asl., with rainfall type of C (Schmidt &  Fergusson.  The  experiment  was    arranged  according  to  completely randomized  block  design  with  four  replications  to  evaluate  effect  of  ninecombination  treatments  of  application  rates  at   application  depths  of  50,  100, and 150 cm. The  range of organic fertilizers rates were  0 - 13.5 kg/tree/year. The experiment revealed that cow dung manure applications on Arabica coffee cultivated  on  Andisols  significantly  increased  yield  at  the  average  of  33% compared  to  the  untreated  crop.  No  significant  effect  of  the  treatment  onvariables of leaf water deficit and soil moisture content during dry season and root  density.  At  range  of  application  depths  of  50  -  150  cm,  the  deeper  the organic matter applications, the higher the yield will be.Key words: Andisols, Arabica coffee, organic matter, cow dung manure

  14. Can Coffee Chemical Compounds and Insecticidal Plants Be Harnessed for Control of Major Coffee Pests?

    Science.gov (United States)

    Green, Paul W C; Davis, Aaron P; Cossé, Allard A; Vega, Fernando E

    2015-11-04

    Pests and pathogens threaten coffee production worldwide and are difficult to control using conventional methods, such as insecticides. We review the literature on the chemistry of coffee, concentrating on compounds most commonly reported from Coffea arabica and Coffea canephora. Differences in chemistry can distinguish coffee species and varieties, and plants grown under different biogeographic conditions exhibit different chemotypes. A number of chemical groups, such as alkaloids and caffeoylquinic acids, are known to be insecticidal, but most studies have investigated their effects on coffee quality and flavor. More research is required to bridge this gap in knowledge, so that coffee can be bred to be more resistant to pests. Furthermore, we report on some pesticidal plants that have been used for control of coffee pests. Locally sourced pesticidal plants have been underutilized and offer a sustainable alternative to conventional insecticides and could be used to augment breeding for resilience of coffee plants.

  15. Synthèse des résultats de recherche sur l'agronomie du caféier arabica (Coffea arabica L. au Rwanda au 31 mars 1994

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    Rutunga, V.

    1999-01-01

    Full Text Available Synthesis of Results on Coffee Agronomy Research in Rwanda on 31 March 1994. Arabica coffee plays an important role in the macro-economic performance of Rwanda. As a result, Agricultural Research Institutes have done research on coffee agronomy, aimed at improving coffee productivite The ecological conditions for coffee in Rwanda are characterized by inadequate rainfall and high light intensity. Soil parental material and chemical properties are variable amongst different small holders coffee producers. The available results indicate that spacing in coffee planting of 2 m x 2 m or 2, 5 m x 1, 5 m are convenient. Higher density with "multicaule"planting provided better production with some varieties. Pruning regime has been established but should still be correctly followed. Mulching is the best cultural technique to improve production. It improves soil physical and chemical properties and water storage. It can also improve minerai fertilizer use efficiency. The mulching materials are variable but not enough for ail coffee plantations. Mulch is applied late (July, August in Rwanda. Living cover with different species including legumes cannot replace efficiently the mulch, although the cover of Desmodium sp. and other few species can offer some advantages. Shade trees in coffee plantations have not been successful. N fertilizer followed by K improved coffee production. The effect of P was not clear while Mg deficiency was observed in some plantations. The formula of N.P.K. and N.P.K. Mg. were recommended but their rates should vary according to the sites. Finally, a beneficial effect was observed in using rotted coffee rinds compost.

  16. Climate Change Impacts on Worldwide Coffee Production

    Science.gov (United States)

    Foreman, T.; Rising, J. A.

    2015-12-01

    Coffee (Coffea arabica and Coffea canephora) plays a vital role in many countries' economies, providing necessary income to 25 million members of tropical countries, and supporting a $81 billion industry, making it one of the most valuable commodities in the world. At the same time, coffee is at the center of many issues of sustainability. It is vulnerable to climate change, with disease outbreaks becoming more common and suitable regions beginning to shift. We develop a statistical production model for coffee which incorporates temperature, precipitation, frost, and humidity effects using a new database of worldwide coffee production. We then use this model to project coffee yields and production into the future based on a variety of climate forecasts. This model can then be used together with a market model to forecast the locations of future coffee production as well as future prices, supply, and demand.

  17. Dependencia del café Coffea arabica L. var. Colombia por la micorriza vesiculo-arbuscular

    Directory of Open Access Journals (Sweden)

    Estrada M. Guillermo

    1995-06-01

    Full Text Available

    In this work it was determined the degree of dependence of the Colombia variety for VAM with different P levels. For that were made two bioassay in nutritional solutions, using river sand as substrate, and in natural soil, and uninfected, coming from Darién (Valle coffee zone. Treatments using nutritional solutions formed a factorial 2 by 5, with and without inoculation plus 5 P levels (20, 30, 40, 50 and 80 ppm. Glomus manihot was inoculated having 200 spores per plant, applied to the root when the were transplanted, Random complete blocks in split plots was the design used. Determinations were made at 60, 120 and 180 days after transplants were made. Results in nutritional solutions showed that small coffee plants var. Colombia, have different dependence degrees to the 20 and 30 P ppm and were absolutely dependent to VAM. In soil, at 180 days, the development of inoculated plants was greater for all P levels, In uninfected soil the plants do not take P and do not grow, except in they were inoculated; such high VAM dependence was evident also in high P levels.

    Para determinar el grado de dependencia de la variedad Colombia por MVA a diferentes niveles de P se realizaron 2 bioensayos: en soluciones nutritivas, con arena de río como sustrato, y en suelo natural y des infestado procedente de la zona cafetera de Darién - Valle. Los tratamientos en soluciones nutritivas conformaron un factorial 2 x 5 con y sin inoculo y 5 niveles de P (20, 30, 40, 50 y 80 ppm; en suelo, un factorial de 4 x 5 sustrato-condición de suelo y 5 niveles de P (5, 15, 30, 50 Y80 ppm. Se inoculó con Glomus manihot a razón de 200 esporas/planta, aplicadas a la raíz al momento del trasplante. En ambos ensayos se utilizó un diseño de bloques completos al azar con arreglo en parcelas divididas. Se efectuaron determinaciones a los 60, 120 y 180 días después del trasplante a bolsa. En soluciones nutritivas, las plantas presentaron grados de dependencia diversos: a

  18. Inoculación de Consorcios Micorrícicos Arbusculares en Coffea arabica, Variedad Caturra en la Región San Martín

    OpenAIRE

    Del Aguila, Karen M.; Vallejos-Torres, Geomar; Arévalo, Luis A.; Becerra, Alejandra G.

    2018-01-01

    Resumen El objetivo del presente estudio fue determinar el efecto de nueve consorcios micorrícicos arbusculares y la inoculación a plántulas de café Coffea arabica, variedad Caturra, comparados con un control (sin inoculación), durante siete meses en vivero. El cultivo de café en la Región San Martín es una de las actividades agrícolas económicas más importantes. Una alternativa para incrementar la producción consiste en la aplicación de hongos micorrizas. Se empleó un Diseño Completamente al...

  19. Differences in Arbuscular Mycorrhizal Fungi among Three Coffee Cultivars in Puerto Rico

    OpenAIRE

    Lebrón, Ligia; Lodge, D. Jean; Bayman, Paul

    2012-01-01

    Mycorrhizal symbiosis is important for growth of coffee (Coffea arabica), but differences among coffee cultivars in response to mycorrhizal interactions have not been studied. We compared arbuscular mycorrhizal (AM) extraradical hyphae in the soil and diversity of AM fungi among three coffee cultivars, Caturra, Pacas, and Borbón, at three farms in Puerto Rico. Caturra had significantly lower total extraradical AM hyphal length than Pacas and Borbón at all locations. P content did not differ a...

  20. Optimizing of Arabica Coffee Bean Fermentation Process Using a Controlled Fermentor

    Directory of Open Access Journals (Sweden)

    Sukrisno Widyotomo

    2015-11-01

    Full Text Available One  of  primary  coffee  processing  steps  which  affect  the  end  quality  isfermentation.  Fermentation  using  a  controlled  fermentor  might  be  usefulbecause  all  of  parameters  which  influence  coffee  quality  can  be  controlled.The  aim of this  research is to evaluate  performance  of  controlled fermentor forfermentation  process  of  Arabica  coffee  beans.  Main  material  of  this  researchwas ripe Arabica coffee cherries from Andungsari Research Station in Bondowoso district.  Research  parameters  were  temperature  with  four  levels  i.e.:  ambient temperature,  30o C,  35oC  and  40oC,  and  fermentation  time  with  three  levels  i.e.: 6  hours,  12  hours,  and  18  hours.  A  horizontal  type  of  modified  fermentor  has been  tested  with  20  kg/batch  or  50%  of  maximum  loading  capacity.  The  result showed  that  an  electric  heater  as  energy  source  can  raise  temperature  duringfermentation  process.  Fermentation  process  using  fermentor  at  30-40oC had  not  significant  effect  on  physical  properties  change  such  as  density,  beancount  per  100  g  and  distribution  of  beans.  Optimum  condition  for  Arabica fermentation  process  in  a  modified  fermentor  reactor  was  25oC  temperature, and  12  hours  fermentation  time.  By  this  condition,  green  beans  have  good organoleptic  score  than  other  fermentation  process  treatments. Key words: Fermentor, fermentation, coffee, quality, organoleptic, horizontal cylinder.

  1. BAP, 2,4-D e ácido acetilsalicílico na indução e diferenciação de calos em anteras de Coffea arabica L BAP, 2,4-D and acetyl-salicylic acid on the callus induction and differentiation in Coffea arabica L. anthers

    Directory of Open Access Journals (Sweden)

    Adelaide Siqueira Silva

    2009-10-01

    Full Text Available O melhoramento genético do cafeeiro por meio de métodos convencionais é um processo demorado para se obter uma nova cultivar. A redução desse tempo é possível através da produção de linhagens homozigóticas, oriundas de dihaplóides obtidas através da cultura de anteras. Objetivou-se aplicar a técnica da cultura de anteras em diferentes cvs. de Coffea arabica L. para induzir a formação de calos e regenerar plântulas di-haplóides, com uso de reguladores vegetais. Os experimentos foram conduzidos no laboratório de Biotecnologia Vegetal da Universidade Federal de Uberlândia (UFU. Anteras das cultivares Mundo Novo LCP-379-19 e Catuaí Vermelho H2077-2-5-44 foram inoculadas em meio MS suplementado com 2,0 mg L-1 de 2,4-D e AAS, nas concentrações de 0; 8; 16; 32 e 64 mg L-1. Calos de 'Catuaí Vermelho 44' foram subcultivados em meio MS acrescido de diferentes concentrações de BAP (0; 2; 4 e 8 mg L-1 e 2,4-D (0; 1; 2 e 4 mg L-1. Tanto para as cvs. Mundo Novo quanto para Catuaí Vermelho 44 o aumento das concentrações de AAS diminuiu a formação de próembrióides nos calos e somente o 2,4-D foi capaz de promover a formação de calos friáveis, porém o equilíbrio da auxina e da citocinina utilizadas no trabalho, favoreceram a produção de calos friáveis.Coffee plant breeding through conventional methods demands a long time to obtain new cultivars. The reduction of this period is possible through the production of homozygous lines, from dihaploids obtained via anther culture. The aim of this study was to apply the anther culture technique on different C. arabica L. cultivars to induce calli formation and to regenerate dihaploid seedlings with the use of plant growth regulators. The experiments were accomplished in the Plant Biotechnology laboratory at Uberlândia Federal University (UFU. Anthers of the cultivars Mundo Novo LCP-379-19 and Catuaí Vermelho H2077-2-5-44 were inoculated on MS medium supplemented with 2.0 mg L-1

  2. Effect of shade on Arabica coffee berry disease development: Toward an agroforestry system to reduce disease impact.

    Science.gov (United States)

    Mouen Bedimo, J A; Njiayouom, I; Bieysse, D; Ndoumbè Nkeng, M; Cilas, C; Nottéghem, J L

    2008-12-01

    Coffee berry disease (CBD), caused by Colletotrichum kahawae, is a major constraint for Arabica coffee cultivation in Africa. The disease is specific to green berries and can lead to 60% harvest losses. In Cameroon, mixed cropping systems of coffee with other crops, such as fruit trees, are very widespread agricultural practices. Fruit trees are commonly planted at random on coffee farms, providing a heterogeneous shading pattern for coffee trees growing underneath. Based on a recent study of CBD, it is known that those plants can reduce disease incidence. To assess the specific effect of shade, in situ and in vitro disease development was compared between coffee trees shaded artificially by a net and trees located in full sunlight. In the field, assessments confirmed a reduction in CBD on trees grown under shade compared with those grown in full sunlight. Artificial inoculations in the laboratory showed that shade did not have any effect on the intrinsic susceptibility of coffee berries to CBD. Coffee shading mainly acts on environmental parameters in limiting disease incidence. In addition to reducing yield losses, agroforestry system may also be helpful in reducing chemical control of the disease and in diversifying coffee growers' incomes.

  3. Atrativo para as abelhas Apis mellifera e polinização em café (Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    Darclet Teresinha Malerbo-Souza

    2003-01-01

    Full Text Available O presente experimento foi conduzido em Jaboticabal, SP, e teve como objetivos estudar uma cultura de café (Coffea arabica L., var. Mundo Novo, quanto à biologia floral, a freqüência e comportamento dos insetos na flor, testar o produto Bee-HereR (Hoescht Shering Agrevo do Brasil Ltda quanto a sua atratividade para as abelhas Apis mellifera e verificar a produção de frutos com e sem a visita dos insetos. Para isso, foram verificados o tempo do desenvolvimento e quantidade de açúcar solúvel do néctar das flores; freqüência das visitações dos insetos, no decorrer do dia, por meio de contagem do número de insetos visitando as flores, a cada 60 minutos, das 8 às 17 horas, 10 minutos em cada horário; tempo (em segundos e tipo de coleta (néctar e/ou pólen dos insetos mais freqüentes; perda de botões florais; porcentagem de flores que se transformaram em frutos; tempo de formação e contagem dos grãos de café, observando-se a porcentagem de frutificação em flores visitadas ou não pelos insetos. Também foram realizados testes por pulverização utilizando-se o produto Bee-HereR , diluído em xarope e em água, em diferentes horários. A flor durou, em média, cerca de 3 dias desde sua abertura até o murchamento. A quantidade de açúcares do néctar apresentou diferença significativa entre os horários, sendo maior às 8 horas (em média, 102,18 ± 8,75 mg de carboidratos totais por flor. A abelha A. mellifera foi o inseto mais freqüente nas flores de café, coletando, principalmente, néctar no decorrer do dia. A perda de botões florais causada pelas chuvas foi, em média, 26,50 ± 11,70%. O tempo para a formação do fruto foi 6 meses e o número de frutos decorrentes do tratamento descoberto foi maior (38,79% e 168,38%, em 1993 e 1994, respectivamente que do tratamento coberto. Apesar da eficiência do produto Bee-HereR ser afetada pelas condições climáticas, ele pode ser usado para atrair as abelhas A. mellifera na

  4. P Status In Andisol And P Content In Arabica Coffee Seedling Leaves Due To The Application Of Phosphate Providing Microorganisms And Organic Matters In Bener Meriah District

    Directory of Open Access Journals (Sweden)

    Hifnalisa

    2017-09-01

    Full Text Available Bener Meriah district is one of the arabica coffee producing regions in Indonesia. Most of arabica coffee in Bener Meriah district grown on Andisol. Generally the availability of P in Andisol is very low. Phosphate providing microorganisms and organic matters can be used to increase Andisol P availability. The objective of this study was to examine the effect of the application of phosphate providing microorganisms and organic matters on P status in Andisol and P content in arabica coffee seedlings leaves in Bener Meriah district. The experiment used a randomized block design that consisted of two factors. Factor I was the application of phosphate providing microorganisms consisting of without microorganisms Glomus sp. Kurthia sp. Corynebacterium sp. and Listeria sp. Factor II was the application of organic matters consisting of T. diversifolia and coffee bean skins. The results of the study showed that Glomus sp. Kurthia sp. Corynebacterium sp. and Listeria sp. decreased soil P-retention by 2.38 5.12 7.48 9.17 respectively increased soil P-available by 24.85 36.03 52.79 77.33 respectively and increased P-content in the arabica coffee seedling leaves by 22.22 33.33 37.0372.27 respectively compared to without the application of microorganisms. The application of coffee bean skins resulted in lower soil P-retention higher soil P-available and P-content in arabica coffee seedling leaves than T. diversifolia. The application of Listeria sp.-coffee bean skins resulted in the lowest soil P-retention the highest soil P-available and P-content in arabica coffee seedlings leaves.

  5. Progresso da ferrugem e da cercosporiose em cafeeiro (coffea arabica l. Com diferentes épocas de início e parcelamentos da fertirrigação Progress of coffee rust and brown eye spot with differents periods at initial irrigation and fertirrigation time

    Directory of Open Access Journals (Sweden)

    Viviane Talamini

    2003-02-01

    .Evaluations of incidence of rust (Hemileia vastatrix Berk. & Br. and brown eye spot (Cercospora coffeicola Berk. & Cooke took place at intervals of 14 days, observing 6 leaves per plant during the period from March 21, 1998 to February 6, 1999 in a 'Catuai' culture. The objective was to evaluate the effect of irrigation and fertirrigation on the incidence of rust and in 12 year coffee plants and analyze the progress curve of these diseases and their correlation to climatic variables. The experimental design was the one of randomized blocks with four treatments of different fertilizer splitting (1: 12 hand applications, 2:12 applications via fertirrigation, 3: 24 fertirrigated applications, 4: 36 fertirrigated applications. The plots were divided in 3 subplots that received treatments in different periods at initial irrigation (subplots A: June 1, B: July 15, C: September 1, and 2 checks (additional treats 1: fertilized 4 times and not irrigated, additional treats 2: conventional fertilizer and not irrigated. The area under disease progress curve (AUDPC was calculated and submitted to variance analysis and Tukey's test at 5% probability. For the coffee rust, no treatment was significant. Curve progress showed maximum incidences in the months of July and September. There was a significant correlation at 1% probability among all the treatments and climatic variables, with the exception of the maximum temperature. For brown eye spot, the treatments attributed to the plots and to the subplots were significant at 1% of probability. However, the interaction between the two was non-significant. Greater AUDPC in splitting fertilizer 2 and 4 was observed. In the subplots, greater AUDPC in check E following for D was observed. Curve progress showed maximum incidences from May to July. There was a significant correlation of brown eye spot incidence in the majority of the treatments in relation to climatic variables.

  6. Fumonisin B2 production by Aspergillus niger in Thai coffee beans

    DEFF Research Database (Denmark)

    Noonim, P.; Mahakarnchanaku, W.; Nielsen, Kristian Fog

    2009-01-01

    During 2006 and 2007, a total of 64 Thai dried coffee bean samples (Coffea arabica) from two growing sites in Chiangmai Province and 32 Thai dried coffee bean samples (Coffea canephora) from two growing sites in Chumporn Province, Thailand, were collected and assessed for fumonisin contamination...... by black Aspergilli. No Fusarium species known to produce fumonisin were detected, but black Aspergilli had high incidences on both Arabica and Robusta Thai coffee beans. Liquid chromatography (LC) with high-resolution mass spectrometric (HRMS) detection showed that 67% of Aspergillus niger isolates from...... coffee beans were capable of producing fumonisins B2 (FB2) and B4 when grown on Czapek Yeast Agar with 5% NaCl. Small amounts (1-9.7 ng g-1) of FB2 were detected in seven of 12 selected coffee samples after ion-exchange purification and LC-MS/MS detection. Two samples also contained FB4...

  7. Multi-scale measurements show limited soil greenhouse GAS emissions in Kenyan smallholder coffee-dairy systems

    DEFF Research Database (Denmark)

    Ortiz-Gonzalo, Daniel; de Neergaard, Andreas; Vaast, Philippe

    2018-01-01

    the three main cropping systems found in the area: 1) coffee (Coffea arabica L.); 2) Napier grass (Pennisetum purpureum); and 3) maize intercropped with beans (Zea mays and Phaseolus vulgaris). Within these fields, chambers were allocated on fertilised and unfertilised locations to capture spatial...

  8. Evaluasi Sifat Biologi Tanah Tanaman Kopi Arabika (Coffea arabica L.) di Beberapa Kecamatan di Kabupaten Mandailing Natal

    OpenAIRE

    Matondang, Daniel

    2016-01-01

    Coffee is one of the agricultural commodities that has a sufficiently high economic value. Mandailaing Natal is the one of the coffee-producing areas in Indonesia. This study aimed to evaluate the biological properties of the soil, among others organic carbon, total nitrogen, total soil respiration and total soil microorganisms to the production of coffee. This study conducted using survey method. Samples were taken at random method deliberately based on deployment coffee plants from various ...

  9. Coffea arabica L., a new host plant for Acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria.

    OpenAIRE

    Jimenez-Salgado, T; Fuentes-Ramirez, L E; Tapia-Hernandez, A; Mascarua-Esparza, M A; Martinez-Romero, E; Caballero-Mellado, J

    1997-01-01

    Acetobacter diazotrophicus was isolated from coffee plant tissues and from rhizosphere soils. Isolation frequencies ranged from 15 to 40% and were dependent on soil pH. Attempts to isolate this bacterial species from coffee fruit, from inside vesicular-arbuscular mycorrhizal fungi spores, or from mealybugs (Planococcus citri) associated with coffee plants were not successful. Other acid-producing diazotrophic bacteria were recovered with frequencies of 20% from the coffee rhizosphere. These N...

  10. Production of herbicide-resistant coffee plants (Coffea canephora P. via Agrobacterium tumefaciens-mediated transformation

    Directory of Open Access Journals (Sweden)

    Alessandra Ferreira Ribas

    2006-01-01

    Full Text Available Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supplemented with 5µM N6 - (2-isopentenyl-adenine (2-iP. Ten µM ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed with up to 1600 mg.L-1 of FinaleTM, a herbicide containing glufosinate as the active ingredient, retained their pigmentation and continued to grow normally during ex vitro acclimation.Plantas transgênicas de Coffea canephora P resistentes ao herbicida glufosinato de amônio foram regeneradas a partir de explantes foliares co-cultivados com Agrobacterium tumefaciens EHA105 contendo o plasmídio pCambia3301 que contém os genes bar e uidA ambos sob controle do promotor 35S. Embriogênese somática direta foi induzida no meio contendo ¼ da concentração de macro, metade da concentração de micronutrientes do meio MS, constituintes orgânicos do meio B5 e 30 g.L-1 de sacarose suplementado com 5µM N6 - (2-isopentenil-adenina (2-iP e 10 µM de glufosinato de amônio para seleção de embriões transgênicos putativos. A presença e a integração do gene bar foram confirmados pelas análises de PCR e Southern blot. As plantas transgênicas selecionadas de café, pulverizadas com 1600 mg.L-1 do herbicida FinaleTM que contém glufosinato como ingrediente ativo, mantiveram a coloração e continuaram crescendo normalmente na aclimatação ex vitro.

  11. Uma nova forma de Coffea

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    C. A. Krug

    1950-01-01

    úmero diplóide de C. Dewevrei, o que parece confirmado pelos resultados obtidos nas hibridações com C. arabica. Apresentam-se também algumas informações preliminares sôbre a sua constituição genética, derivadas de um extenso projeto de cruzamentos com espécies e também genótipos diferentes de C. arabica (nana; prpr; FsFs; lrlr; momo; MgMg; ErEr; C-; CtCt; cece; etc. Concluiu-se que a nova forma é extremamente heterozigota, com relação aos fatôres determinantes dos seus principais caracteres morfológicos, e que possui vários alelos dos fatôres conhecidos de C. arabica, apresentando os indivíduos F1, e os "backcrosses" com esta espécie, acentuada predominância dos caracteres da nova forma. A auto-esterilidade predomina nas gerações de "backcrosses" seguidos, já se tendo encontrado, entretanto, plantas razoavelmente auto-férteis. Além de constituir material básico de grande importância para as tentativas de síntese de novos tipos de cafeeiros, a forma em questão também está sendo ensaiada em plantações mistas, intercalando-se o seu clone (cafeeiros enxertados com fileiras de cafeeiros arábica (bourbon, etc., que servirão de agentes polinizadores. Conclui-se que a descoberta dêsse cafeeiro, em Terra Roxa, facilitará a solução do problema da formação de novos cafezais em terras extremamente esgotadas.In the past 18 years coffee breeding investigations by the Genetics Division of the Instituto Agronômico have been largeiy confined to the species C. arabica because it is the species that produces coffee of the best marketable qualities. Coffee cultivation in depleted soils requires the use of very vigorous plants. Vigorous plant growth is more frequently found in some diploid species of Coffea. Artificial hybridization of these diploid species with the tetraploid species C. arabica has been tried in several of the Institutes' Experiment Stations where breeding work on coffee is underway. Small scale breeding tests have also been carried

  12. Quantification of the Robusta fraction in a coffee blend via Raman spectroscopy: proof of principle.

    Science.gov (United States)

    Wermelinger, Thomas; D'Ambrosio, Lucio; Klopprogge, Babette; Yeretzian, Chahan

    2011-09-14

    Among the 100 different known Coffea species, Coffea arabica L. (Arabica) and Coffea canephora Pierre (Robusta) are the only two of commercial interest. They differ in a range of agronomic, genetic, and chemical properties. Due to the significant price difference between Arabica and Robusta, there is an economic incentive to illicitly replace Arabica with Robusta. Therefore, it is crucial to have accurate methods to determine the Robusta-to-Arabica-ratio in blends. This paper presents the proof of principle of a new and fast approach to determine the Robusta fraction in a blend based on Raman spectroscopy. The oils of two references (a pure Robusta and pure Arabica coffee) and six blends thereof consisting of different Robusta and Arabica fractions were extracted using a Soxhlet system. The solutes were analyzed by means of Raman spectroscopy without further workup. Using the intensity ratio between two Raman peaks, one characteristic for kahweol and one characteristic for fatty acids, allowed determinination of the Robusta content in a given mixture. The intensity ratio is linearly dependent on the Robusta content of the compound. Above a Robusta content of 75 wt %, kahweol was not detectable. The Raman data are in agreement with results obtained from the very time-consuming multistep DIN 10777 procedures based on HPLC.

  13. Climate-based statistical regression models for crop yield forecasting of coffee in humid tropical Kerala, India

    Science.gov (United States)

    Jayakumar, M.; Rajavel, M.; Surendran, U.

    2016-12-01

    A study on the variability of coffee yield of both Coffea arabica and Coffea canephora as influenced by climate parameters (rainfall (RF), maximum temperature (Tmax), minimum temperature (Tmin), and mean relative humidity (RH)) was undertaken at Regional Coffee Research Station, Chundale, Wayanad, Kerala State, India. The result on the coffee yield data of 30 years (1980 to 2009) revealed that the yield of coffee is fluctuating with the variations in climatic parameters. Among the species, productivity was higher for C. canephora coffee than C. arabica in most of the years. Maximum yield of C. canephora (2040 kg ha-1) was recorded in 2003-2004 and there was declining trend of yield noticed in the recent years. Similarly, the maximum yield of C. arabica (1745 kg ha-1) was recorded in 1988-1989 and decreased yield was noticed in the subsequent years till 1997-1998 due to year to year variability in climate. The highest correlation coefficient was found between the yield of C. arabica coffee and maximum temperature during January (0.7) and between C. arabica coffee yield and RH during July (0.4). Yield of C. canephora coffee had highest correlation with maximum temperature, RH and rainfall during February. Statistical regression model between selected climatic parameters and yield of C. arabica and C. canephora coffee was developed to forecast the yield of coffee in Wayanad district in Kerala. The model was validated for years 2010, 2011, and 2012 with the coffee yield data obtained during the years and the prediction was found to be good.

  14. Purine alkaloid formation and CO2 gas exchange in dependence of development and of environmental factors in leaves of Coffea arabica L.

    Science.gov (United States)

    Frischknecht, P M; Eller, B M; Baumann, T W

    1982-12-01

    In the leaves of Coffea arabica L., purine alkaloid formation was estimated by analyzing the theobromine and caffeine content and by measuring the methylation rate of [2-(14)C]theobromine to [2-(14)C]caffeine in short-term experiments (6-24 h). At the same time, growth (in terms of dry weight and area), net photosynthesis (NPS), and dark respiration were determined. During leaf development, which was considered to be terminated when NPS was at a maximum (60-80 μmol g(-1) s(-1)) and dark respiration at a minimum (5-7.5 μmol g(-1) s(-1)), the content of theobromine and the velocity of caffeine formation were both found to decrease by a factor of more than 100. The close correlation between the theobromine content and the methylation rate is suspended when purine alkaloid formation is influenced by factors other than leaf development. Among these factors, temperature is the most effective: the velocity of caffeine biosynthesis is increased by raising the temperature and vice versa. Although the plants were well irrigated, a drastic decrease of NPS in the afternoon was observed under all environmental conditions tested. Light saturation was reached between 170-360 μmol m(-2) s(-1). The temperature optimum of NPS was shown to be very broad (24-33°C)m provided the adaptation time was sufficiently long.

  15. Cuantificación de minerales K, Ca, Mg y P en pulpa y pergamino de café (Coffea arabica L. var. Typica

    Directory of Open Access Journals (Sweden)

    Johanna Mendoza Abarca

    2010-01-01

    Full Text Available Se trabajó con muestras de café Coffea arabica L., variedad Typica de tres lugares en Ecuador: Palanda, Vilcabamba y El Pangui, seleccionados por su disponibilidad y condiciones geográficas. Se analizó potasio, calcio y magnesio por espectrofotometría de absorción atómica; y fósforo por espectrofotometría visible, utilizando en todos los casos la digestión ácida para la preparación de las muestras. Con el objetivo de mantener la trazabilidad de los residuos se realizó el beneficio húmedo del café. Los resultados obtenidos para la pulpa de café expresados en base seca fueron: K 3,1 ± 0,43 %; Ca 0,46 ± 0,06 %; Mg 0,14 ± 0,01 % y P 0,13 ± 0,01 %. Para pergamino de café: K 0,16 ± 0,02 %; Ca 0,14 ± 0,05 %; Mg 0,06 ± 0,01 % y P 0,02 ± 0,01 %. En adición, se determinaron los minerales en el grano de café. Con estos resultados podemos mencionar que la pulpa de café es una buena fuente de potasio (especialmente, magnesio y fósforo. El pergamino pudiera ser considerado como fuente de fósforo.

  16. CONSTRUCCIÓN DE UNA BIBLIOTECA GENÓMICA DE Coffea Arabica Var. COLOMBIA Y EVALUACIÓN CON UNA SECUENCIA HOMÓLOGA A UBIQUITINA

    Directory of Open Access Journals (Sweden)

    Elsa Leonor Álvarez Méndez 1, 2,

    2004-06-01

    Full Text Available Con el fin de buscar secuencias de interés en el genoma de Coffea arabica var. Colombia, se construyó una biblioteca genómica en el vector Lambda FIX II (Stratagene con un tamaño promedio de inserto de 15Kb y con un título de 1,33x106 ufp/ml que representa aproximadamente 3,7 veces el genoma haploide.La biblioteca fue evaluada utilizando como sonda un producto de PCR amplificado con la combinación de iniciadores InhF - R631 y que presenta alta homología con secuencias tipo ubiquitinas de Arabidopsis thaliana y Oriza sativa. Se identificaron dos clones recombinantes (cof-ubi1 y cof-ubi2 que hibridizaron con la sonda tipo ubiquitina. Los resultados obtenidos indican que la biblioteca genómica es adecuada para la identificación de secuencias de interés, mapeo genético y estudios sobre regulación de la expresión de genes.

  17. Cuantificación de minerales K, Ca, Mg y P en pulpa y pergamino de café (Coffea arabica L. var. Typica

    Directory of Open Access Journals (Sweden)

    Jorge Geovanny Figueroa Hurtado

    2010-12-01

    Full Text Available Se trabajó con muestras de café Coffea arabica L., variedad Typica de tres lugares en Ecuador: Palanda, Vilcabamba y El Pangui, seleccionados por su disponibilidad y condiciones geográficas. Se analizó potasio, calcio y magnesio por espectrofotometría de absorción atómica; y fósforo por espectrofotometría visible, utilizando en todos los casos la digestión ácida para la preparación de las muestras. Con el objetivo de mantener la trazabilidad de los residuos se realizó el beneficio húmedo del café. Los resultados obtenidos para la pulpa de café expresados en base seca fueron: K 3,1 ± 0,43 %; Ca 0,46 ± 0,06 %; Mg 0,14 ± 0,01 % y P 0,13 ± 0,01 %. Para pergamino de café: K 0,16 ± 0,02 %; Ca 0,14 ± 0,05 %; Mg 0,06 ± 0,01 % y P 0,02 ± 0,01 %. En adición, se determinaron los minerales en el grano de café. Con estos resultados podemos mencionar que la pulpa de café es una buena fuente de potasio (especialmente, magnesio y fósforo. El pergamino pudiera ser considerado como fuente de fósforo.

  18. Isolation, identification and toxigenic potential of ochratoxin A-producing Aspergillus species from coffee beans grown in two regions of Thailand

    DEFF Research Database (Denmark)

    Noonim, P.; Mahakarnchanakul, W.; Nielsen, Kristian Fog

    2008-01-01

    In 2006 and 2007, 32 Thai dried coffee bean samples (Coffea arabica) from two growing sites of Chiang Mai Province, and 32 Thai dried coffee bean samples (Coffea canephora var. robusta) from two growing sites of Chumphon Province, Thailand, were collected and assessed for the distribution of fungi...... with the potential to produce ochratoxin A (OTA). The overall percentage of fungal contamination in coffee was 98% and reduced to 60% after surface disinfection. There were remarkable ecological differences in the composition of ochratoxigenic species present in these two regions. Arabica coffee bean samples from...... the North had an average of 78% incidence of colonization with Aspergillus of section Circumdati with Aspergillus westerdijkiae and A. melleus as the predominant species. Aspergillus spp. of section Nigri were found in 75% of the samples whereas A. ochraceus was not detected. Robusta coffee beans from...

  19. Influence of Environmental Conditions and Genetic Background of Arabica Coffee (C. arabica L) on Leaf Rust (Hemileia vastatrix) Pathogenesis.

    Science.gov (United States)

    Toniutti, Lucile; Breitler, Jean-Christophe; Etienne, Hervé; Campa, Claudine; Doulbeau, Sylvie; Urban, Laurent; Lambot, Charles; Pinilla, Juan-Carlos H; Bertrand, Benoît

    2017-01-01

    Global warming is a major threat to agriculture worldwide. Between 2008 and 2013, some coffee producing countries in South and Central America suffered from severe epidemics of coffee leaf rust (CLR), resulting in high economic losses with social implications for coffee growers. The climatic events not only favored the development of the pathogen but also affected the physiological status of the coffee plant. The main objectives of the study were to evaluate how the physiological status of the coffee plant modified by different environmental conditions impact on the pathogenesis of CLR and to identify indicators of the physiological status able to predict rust incidence. Three rust susceptible genotypes (one inbred line and two hybrids) were grown in controlled conditions with a combination of thermal regime (TR), nitrogen and light intensity close to the field situation before being inoculated with the rust fungus Hemileia vastatrix . It has been demonstrated that a TR of 27-22°C resulted in 2000 times higher sporulation than with a TR of 23-18°C. It has been also shown that high light intensity combined with low nitrogen fertilization modified the CLR pathogenesis resulting in huge sporulation. CLR sporulation was significantly lower in the F1 hybrids than in the inbred line. The hybrid vigor may have reduced disease incidence. Among the many parameters studied, parameters related to photosystem II and photosynthetic electron transport chain components appeared as indicators of the physiological status of the coffee plant able to predict rust sporulation intensity. Taken together, these results show that CLR sporulation not only depends on the TR but also on the physiological status of the coffee plant, which itself depends on agronomic conditions. Our work suggests that vigorous varieties combined with a shaded system and appropriate nitrogen fertilization should be part of an agro-ecological approach to disease control.

  20. Field-cage evaluation of the parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) as a natural enemy of the coffee berry borer

    Science.gov (United States)

    Phymastichus coffea (Hymenoptera: Eulophidae) is an African parasitoid that has been imported to Mexico and other Latin American countries for the biological control of the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae). As a part of the evaluation of this ...

  1. Observações Citológicas em coffea: VI — Desenvolvimento do embrião e do endosferma em Coffea Arabica l.

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    A. J. T. Mendes

    1942-04-01

    Full Text Available The ovule of C. arabica L. consists õf a single integument and a small nucellús which disappears as the ovule matures. Three of the four macrospores resulting from the'division of the macrosporocyte, degenerate. The remaining chalazal cell gives rise to a "normal'' embryo sac, which is ready for fertilization at the time of the flower opening. Double fertilization occurs, as a rule, the day the flower opens. The embryo sac then increases in volume and compresses the inner integument cells. The outer cells of the integument, however, multiply actively, giving rise to the "perisperm". After degeneration of the synergids and antipodals, the zygote stays near the micro-pyle in a resting stage, while the primary endosperm nucleus divides. This first division of the endosperm occurs from 21 to 27 days after flower opening. The cytoplasm condenses around the newly formed nuclei, permitting the adjacent tissues to sink into the embryo sac. Since the separating walls were not seen at the binueleate stage and were present at the four-nucleate stage, it seems that the endosperm belongs to the' "nuclear type". As the number of endosperm cells increases, the "perisperm" cells are again compressed and give more and more room to the new tissue. The first division in the zygote occurs from sixty to seventy days after flower opening, when the endosperm is already multinucleate. A differentiated embryo develops, with a hypocotyl and two small cotyledons in the ripe seed. In the ripe seed the "perisperm" disappears almost completely: its remains form the thin "silver skin" which envelops the endosperm. The parchment layer which envelops the seed is the endocarp.

  2. Post-harvest practices linked with ochratoxin A contamination of coffee in three provinces of Cordillera Administrative Region, Philippines.

    Science.gov (United States)

    Barcelo, Jonathan M; Barcelo, Racquel C

    2018-02-01

    One of the emerging concerns in the Cordillera Administrative Region, Philippines is ochratoxin A (OTA) contamination in coffee. During 2015 to 2016, a total of 51 Arabica (Coffea arabica) coffee samples from Benguet province and 71 Robusta (Coffea canephora var. Robusta) coffee samples from the provinces of Ifugao and Kalinga were analysed for OTA contamination. The OTA-producing fungal contaminants during drying and storage of Arabica and Robusta coffee were Aspergillus niger and Aspergillus ochraceus. Ochratoxin A was more commonly detected in Robusta coffee (36.6%) than in Arabica coffee (21.6%). Among the contaminated samples, Robusta coffee cherries in the drying yard had the highest mean OTA level (120.2 μg kg -1 , n = 10) while roasted Robusta coffee beans had the lowest mean level (4.8 μg kg -1 , n = 9). The onset of contamination of Arabica coffee occurred during storage, with a mean OTA level of 46.7 μg kg -1 (n = 9). Roasted coffee had lower OTA content although five samples had levels >5.0 μg kg -1 . Pearson Chi-square analysis (χ 2 ) and Fisher's exact test revealed that several post-harvest practices involving non-removal of the husk or hull and mixing of defective coffee were significantly associated with the occurrence of OTA during drying and storage (p coffee in all stages of post-harvest and rapid reduction of moisture content particularly during drying.

  3. Melhoramento do cafeeiro: XXIII - Novos dados sôbre a variabilidade em linhas isogênicas de café Coffee breeding: XXIII variability in isogenic lines of cofeea arabica

    Directory of Open Access Journals (Sweden)

    L. C. Mônaco

    1964-01-01

    turned to yield and plant height variability since among the progenies there were two isogenic lines obtained by doubling the chromosomes of two haploid coffee trees and the hybrid between them. Concerning plant height the variability of the hybrid was similar to that of both isogenic parents indicating no relation between heterozygosis for factors controlling plant height and the uniformity of the progenies. The yield variation of the hybrid was found to be higher,intermediate or lower than the parents depending on the year considered, but the differences between the variances were not statistically significant. The information so far gathered about genetic variability and homeostasis in coffee indicate that heterozygosity seems not to improve the plasticity of the individuals. Natural selection seems to have maintained in the original population genic combinations which would result in better homeostatic strength. It is also possible that the lines studied are genetically not so distinct and only a few loci would be in heterozygous condition. Additional data are required before deciding about the relation between homeostasis and heterozygosis in Coffea arabica.

  4. Transferência do fator caturra para o cultivar Mundo Novo de Coffea arabica Transfer of the CT gene to Mundo Novo cultivar

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    A. Carvalho

    1972-01-01

    Full Text Available No presente trabalho são relatados os estudos realizados visando à introdução do gene Ct (caturra que contribui para reduzir a altura da planta, no cultivar Mundo" Novo de Coffea arabica.Estudaram-se, em ensaios de produtividade, as populações Fv F.,, F3 e F4. Nessas populações e principalmente entre os descendentes dos "caféeiros H 2077-2-5 e H 2077-2-12, foram selecionadas plantas homozigotas para os alelos Ct e também para os alelos responsáveis pela cor do fruto xc ou Xc. Essas combinações foram denominadas 'Catuaí Amarelo' e 'Catuaí Vermelho', respectivamente, e suas características são apresentadas. Os novos cultivares vêm-se mostrando de interesse econômico para as regiões cafeeiras não somente pelo porte pequeno, mas também pela produtividade, pelo vigor vegetativo e pela precocidade.The successful transfer of the Ct gene for short internode to the tall cultivar of Coffea arábica'Mundo Novo' is reported. Individual selections were carried out in the F1, F2, F3 and F4 generations. It was found that early selection in the F2 generation was quite effective. A remarkably good correlation was found between productitivity of F2 plants and the yield of the F3 and F4 generations. Plants of the F4 generation have shown reasonable uniformity and high yield in several trials. The new selections showed to be early producers. Two new cultivars were released namely 'Catuaí Amarelo' and 'Catuaí Vermelho'. The former has yellow fruits whereas the latter has red fruits. The plants are much shorter that the ones of Mundo Novo. The new cultivars have a very strong secondary and tertiary branching. Because of these characteristics Catuaí Amarelo and Catuaí Vermelho are being planted in large scale replacing the tall cultivars.

  5. Coffea arabica L., a new host plant for Acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria.

    Science.gov (United States)

    Jimenez-Salgado, T; Fuentes-Ramirez, L E; Tapia-Hernandez, A; Mascarua-Esparza, M A; Martinez-Romero, E; Caballero-Mellado, J

    1997-09-01

    Acetobacter diazotrophicus was isolated from coffee plant tissues and from rhizosphere soils. Isolation frequencies ranged from 15 to 40% and were dependent on soil pH. Attempts to isolate this bacterial species from coffee fruit, from inside vesicular-arbuscular mycorrhizal fungi spores, or from mealybugs (Planococcus citri) associated with coffee plants were not successful. Other acid-producing diazotrophic bacteria were recovered with frequencies of 20% from the coffee rhizosphere. These N2-fixing isolates had some features in common with the genus Acetobacter but should not be assigned to the species Acetobacter diazotrophicus because they differed from A. diazotrophicus in morphological and biochemical traits and were largely divergent in electrophoretic mobility patterns of metabolic enzymes at coefficients of genetic distance as high as 0.950. In addition, these N2-fixing acetobacteria differed in the small-subunit rRNA restriction fragment length polymorphism patterns obtained with EcoRI, and they exhibited very low DNA-DNA homology levels, ranging from 11 to 15% with the A. diazotrophicus reference strain PAI 5T. Thus, some of the diazotrophic acetobacteria recovered from the rhizosphere of coffee plants may be regarded as N2-fixing species of the genus Acetobacter other than A. diazotrophicus. Endophytic diazotrophic bacteria may be more prevalent than previously thought, and perhaps there are many more potentially beneficial N2-fixing bacteria which can be isolated from other agronomically important crops.

  6. Chemical characterisation of non-defective and defective green arabica and robusta coffees by electrospray ionization-mass spectrometry (ESI-MS).

    Science.gov (United States)

    Mendonça, Juliana C F; Franca, Adriana S; Oliveira, Leandro S; Nunes, Marcella

    2008-11-15

    The coffee roasted in Brazil is considered to be of low quality, due to the presence of defective coffee beans that depreciate the beverage quality. These beans, although being separated from the non-defective ones prior to roasting, are still commercialized in the coffee trading market. Thus, it was the aim of this work to verify the feasibility of employing ESI-MS to identify chemical characteristics that will allow the discrimination of Arabica and Robusta species and also of defective and non-defective coffees. Aqueous extracts of green (raw) defective and non-defective coffee beans were analyzed by direct infusion electrospray ionization mass spectrometry (ESI-MS) and this technique provided characteristic fingerprinting mass spectra that not only allowed for discrimination of species but also between defective and non-defective coffee beans. ESI-MS profiles in the positive mode (ESI(+)-MS) provided separation between defective and non-defective coffees within a given species, whereas ESI-MS profiles in the negative mode (ESI(-)-MS) provided separation between Arabica and Robusta coffees. Copyright © 2008 Elsevier Ltd. All rights reserved.

  7. Direct somatic embryogenesis in coffea arabica L. CVS. caturra and catuaí: effect of triacontanol, light condition, and medium consistency

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    Andrés M. Gatica

    2008-01-01

    Full Text Available Se investigó la influencia de la concentración de triacontanol (TRIA y su interacción con el ácido indolacético (AIA en la inducción de la embriogénesis somática directa en Coffea arabica L. cvs. Caturra y Catuaí. Adicionalmente, se evaluó el efecto de la condición de la luz y de la consistencia del medio de cultivo (semisólido vs. líquido. Se determinó que la mayor cantidad de embriones somáticos por explante fue de 3,9±0,5 en Caturra y 3,6±0,5 en Catuaí, en el medio de cultivo Murashige y Skoog (1962 al 50%, complementado con BAP (1,1 µM, IAA (2,85 µM y TRIA (4,55 µM. Para la consistencia del medio, la mayor cantidad de embriones somáticos en Caturra (3,2±0,2 y Catuaí (6,0±0,4 se obtuvo con el medio semisólido de Yasuda et al. (1985. En Catuaí, la mayor cantidad de embrioides se obtuvo con 16 h luz (7,6±1,0 y oscuridad (6,2±0,6 y para Caturra, con oscuridad (4,2±0,4 y 16 h luz (3,8±0,5. No se observó la formación de embriones somáticos en los explantes de Caturra y Catuaí después de 12 semanas de cultivo, en el medio líquido descrito por Yasuda et al. (1985 ni bajo ninguna de las condiciones de luz.

  8. Embryogenic potential and the expression of arabinogalactanproteins (AGPs in embryogenic cell aggregates of Coffea arabica cv. Caturra rojo under influence of CO2

    Directory of Open Access Journals (Sweden)

    Raúl Barbón

    2002-07-01

    Full Text Available The establishment and optimization of regeneration systems via somatic embryogenesis has centered traditionally on the study of the culture medium components, with little attention to other factors of the in vitro environment like the composition of the gaseous atmosphere. This work was realized with the aim to determine the embryogenic potential and the expression of arabinogalactanproteins (AGPs in embryogenic cell aggregates of Coffea arabica cv. Caturra Rojo under influence of carbon dioxide (CO2. Concentrations of 2.5% and 5.0% of CO2 stimulated a greater formation of somatic embryos (307 ES/50mgMF and 277 ES/50mgMF respectively in semisolid culture medium with better results to the controls with passive interchange and forced ventilation (195 ES/50mgMF and 107 ES/50mgMF whereas a concentration of 10.0% of CO2 inhibited the process of somatic embryogenesis (95 ES/50mgMF. In cell suspensions the formation of somatic embryos was greater with a concentration of 2.5% of CO2 (130x103 ES.l-1 in comparison with 5.0% and 10.0% of CO2 (116x103 ESl-1 and 15x103 ES.l-1 and the controls with passive interchange and forced ventilation. With a concentration of 2.5% CO2 there was a greater synthesis of Arabinogalactanproteins (AGPs at the cellular level (0.068 mg.gMF-1 and the excretion in the culture medium (2.10 mg.l-1, which coincided with the highest values of production of somatic embryos. Key Words: embryogenic cell suspensions, in vitro environment, molecular markers, somatic embryo, somatic embryogenesis

  9. Producción de café (Coffea Arabica L. en respuesta al manejo específico por sitio de la fertilidad del suelo

    Directory of Open Access Journals (Sweden)

    Luz Adriana Lince Salazar

    2016-12-01

    Full Text Available El estudio de la variabilidad espacial es el principal insumo para el manejo específico por sitio MES, con el fin de optimizar los recursos, al reducir los costos de producción de los cultivos y el impacto negativo sobre el ambiente. Con el objetivo de determinar el efecto del MES en la producción del café, durante los años 2011 y 2012 se realizó un experimento del manejo de la fertilidad del suelo en café (Coffea arabica L., Variedad Castillo® en lotes con áreas diferentes (1,0, 2,0 y 3,5 ha, ubicados en tres fincas del departamento del Quindío, Colombia. Se evaluó el efecto de tres tratamientos en la producción de café cereza: i testigo sin fertilización; ii manejo de acuerdo a los resultados de los análisis de suelo, logrados mediante un muestreo convencional en zigzag y, iii manejo por sitio específico conforme lo indicaron las áreas homogéneas, obtenidas mediante mapas de variabilidad espacial. En ninguno de los lotes hubo efecto de los tratamientos, respuesta que se relacionó con uno o más de los siguientes factores: tiempo relativamente corto de evaluación, fertilidad del suelo, reservas de los nutrientes en las plantas, y el número reducido de repeticiones (tres por tratamiento.

  10. Producción de café (Coffea arabica L. en respuesta al manejo específico de la fertilidad del suelo

    Directory of Open Access Journals (Sweden)

    Luz Adriana Lince Salazar

    2015-12-01

    Full Text Available El estudio de la variabilidad espacial es el principal insumo para el manejo específico por sitio-MES, con fines de optimizar los recursos, al reducir los costos de producción de los cultivos y el impacto negativo sobre el ambiente. Con el objetivo de determinar el efecto del MES en la producción del café, durante los años 2011 y 2012 se realizó un experimento del manejo de la fertilidad del suelo en café (Coffea arabica L., Variedad Castillo® en lotes con áreas diferentes (1,0, 2,0 y 3,5 ha, ubicados en tres fincas del departamento del Quindío, Colombia. Se evaluó el efecto de tres tratamientos en la producción de café cereza: i testigo sin fertilización; ii manejo de acuerdo a los resultados de los análisis de suelo, logrados mediante un muestreo convencional en zigzag y, iii manejo por sitio específico conforme lo indicaron las áreas homogéneas, obtenidas mediante mapas de variabilidad espacial. En ninguno de los lotes hubo efecto de los tratamientos, respuesta que se relacionó con uno o más de los siguientes factores: tiempo relativamente corto de evaluación, fertilidad del suelo, reservas de los nutrientes en las plantas, y el número reducido de repeticiones (tres por tratamiento.

  11. Crecimiento de café (Coffea arabica L. durante la etapa de almácigo en respuesta a la salinidad generada por fertilizantes

    Directory of Open Access Journals (Sweden)

    Siavosh Sadeghian Khalajabadi

    2014-12-01

    Full Text Available La salinidad del suelo puede afectar negativamente el crecimiento de las plantas, e incluso causar su muerte. Esta investigación tuvo como propósito, determinar el efecto de la salinidad generada por fertilizantes en el crecimiento de café Coffea arabica L. durante la etapa de almácigo. Se recolectaron muestras de suelo en dos Estaciones Experimentales (EE de Cenicafé (Naranjal en Caldas y Timbío en Cauca y una finca cafetera (FC en Jamundí (Valle del Cauca. Bajo el diseño completamente aleatorio se evaluó para cada suelo la respuesta de café variedad Caturra a 27 tratamientos (factorial 33, resultantes de tres dosis de calcio-Ca, de magnesio-Mg y de potasio-K (0, 1 y 3 g dm-3 de suelo, empleando como fuentes sulfatos. La conductividad eléctrica (C.E., como indicador de la salinidad, se incrementó en conformidad a las dosis, con valores más altos para el Mg, seguido por K y Ca, resultado que se relacionó principalmente con el índice de salinidad de los fertilizantes y su solubilidad. Las diferencias de la C.E. entre las localidades (EE Naranjal>EE Timbío>FC Jamundí se atribuyeron a la capacidad de éstos para retener la humedad. Conforme al aumento de la salinidad disminuyó el peso de las plantas, hasta causar síntomas de toxicidad y posterior muerte. El nivel crítico de la C.E., calculado para el 90% del crecimiento relativo, correspondió en promedio a 1,1 dS m-1.

  12. High coffee population density to improve fertility of an oxisol

    OpenAIRE

    Pavan,Marcos Antonio; Chaves,Júlio César Dias; Siqueira,Rubens; Androcioli Filho,Armando; Colozzi Filho,Arnaldo; Balota,Elcio Liborio

    1999-01-01

    The objective of this work was to evaluate the effect of coffee (Coffea arabica L.) population densities on the chemical and microbiological properties of an Oxisol. The work was carried out on soil samples of 0-20 cm depth originated from an experimental site which had been used for coffee tree spacing studies during 15 years, in Paraná State, Brazil. Eight coffee tree populations were evaluated: 7143, 3571, 2381, 1786, 1429, 1190, 1020, and 893 trees/ha. Increasing plant population increase...

  13. [Coupling effects of periodic rewatering after drought stress and nitrogen fertilizer on growth and water and nitrogen productivity of Coffea arabica].

    Science.gov (United States)

    Hao, Kun; Liu, Xiao Gang; Zhang, Yan; Han, Zhi Hui; Yu, Ning; Yang, Qi Liang; Liu, Yan Wei

    2017-12-01

    The effects of periodic rewatering after drought stress and nitrogen fertilizer on growth, yield, photosynthetic characteristics of leaves and water and nitrogen productivity of Coffea arabica (Katim P7963) were studied under different nitrogen application levels in 2.5 consecutive years. Irrigation (periodic rewatering after drought stress) and nitrogen were designed as two factors, with four modes of irrigation, namely, full irrigation (I F-F : 100%ET 0 +100%ET 0 , ET 0 was reference crop evapotranspiration), rewatering after light drought stress (I L-F : 80%ET 0 +100%ET 0 ), rewatering after moderate drought stress (I M-F : 60%ET 0 +100%ET 0 ) and rewatering after severe drought stress (I S-F : 40%ET 0 +100%ET 0 ), and three levels of nitrogen, namely, high nitrogen (N H : 750 kg N·hm -2 each time), middle nitrogen (N M : 500 kg N·hm -2 each time), low nitrogen (N L : 250 kg N·hm -2 each time), and nitrogen was equally applied for 4 times. The results showed that irrigation and nitrogen had significant effect on plant height, stem diameter, yield and water and nitrogen productivity of C. arabica, and plant height and stem diameter showed S-curve with the day ordinal number, and leaf photosynthesis decreased significantly under drought stress but most photosynthesis index recovered somewhat after rewatering. Compared with I F-F , I L-F increased dry bean yield by 6.9%, while I M-F and I S-F decreased dry bean yield by 15.2% and 38.5%, respectively; I L-F and I M-F increased water use efficiency by 18.8% and 6.0%, respectively, while I S-F decreased water use efficiency by 12.1%; I L-F increased nitrogen partial productivity by 6.1%, while I M-F and I S-F decreased nitrogen partial productivity by 14.0% and 36.0%, respectively. Compared with N H , N M increased dry bean yield and water use efficiency by 20.9% and 19.3%, while N L decreased dry bean yield and water use efficiency by 42.4% and 41.9%, respectively; N M and N L increased nitrogen partial

  14. Estimating biophysical properties of coffee (Coffea canephora) plants with above-canopy field measurements, using CropSpec®

    Science.gov (United States)

    Putra, Bayu T. Widjaja; Soni, Peeyush; Morimoto, Eiji; Pujiyanto, Pujiyanto

    2018-04-01

    Remote sensing technologies have been applied to many crops, but tree crops like Robusta coffee (Coffea canephora) under shade conditions require additional attention while making above-canopy measurements. The objective of this study was to determine how well chlorophyll and nitrogen status of Robusta coffee plants can be estimated with the laser-based (CropSpec®) active sensor. This study also identified appropriate vegetation indices for estimating Nitrogen content by above-canopy measurement, using near-infra red and red-edge bands. Varying light intensity and different background of the plants were considered in developing the indices. Field experiments were conducted involving different non-destructive tools (CropSpec® and SPAD-502 chlorophyll meter). Subsequently, Kjeldahl laboratory analyses were performed to determine the actual Nitrogen content of the plants with different ages and field conditions used in the non-destructive previous stage. Measurements were undertaken for assessing the biophysical properties of tree plant. The usefulness of near-infrared and red-edge bands from these sensors in measuring critical nitrogen levels of coffee plants by above-canopy measurement are investigated in this study.

  15. Developing core collections to optimize the management and the exploitation of diversity of the coffee Coffea canephora.

    Science.gov (United States)

    Leroy, Thierry; De Bellis, Fabien; Legnate, Hyacinthe; Musoli, Pascal; Kalonji, Adrien; Loor Solórzano, Rey Gastón; Cubry, Philippe

    2014-06-01

    The management of diversity for conservation and breeding is of great importance for all plant species and is particularly true in perennial species, such as the coffee Coffea canephora. This species exhibits a large genetic and phenotypic diversity with six different diversity groups. Large field collections are available in the Ivory Coast, Uganda and other Asian, American and African countries but are very expensive and time consuming to establish and maintain in large areas. We propose to improve coffee germplasm management through the construction of genetic core collections derived from a set of 565 accessions that are characterized with 13 microsatellite markers. Core collections of 12, 24 and 48 accessions were defined using two methods aimed to maximize the allelic diversity (Maximization strategy) or genetic distance (Maximum-Length Sub-Tree method). A composite core collection of 77 accessions is proposed for both objectives of an optimal management of diversity and breeding. This core collection presents a gene diversity value of 0.8 and exhibits the totality of the major alleles (i.e., 184) that are present in the initial set. The seven proposed core collections constitute a valuable tool for diversity management and a foundation for breeding programs. The use of these collections for collection management in research centers and breeding perspectives for coffee improvement are discussed.

  16. Furan in roasted, ground and brewed coffee

    Science.gov (United States)

    Gruczyńska, Eliza; Kowalska, Dorota; Kozłowska, Mariola; Majewska, Ewa; Tarnowska, Katarzyna

    2018-01-01

    Coffee is the most popular hot beverage in the world. The annual coffee production in 2010, 2014 and 2016 was 8.1, 9.0 and 9.3 million tons respectively. There are more than 100 coffee species, but only two of them: Arabica (Coffea arabica) and Robusta (Coffea canephora) have gained commercial importance. During roasting of green coffee beans not only desirable compounds are formed, that exert positive influence on the taste and flavour of coffee, but also small quantities of undesirable ones. Furan (C4H4O) is one of the latter. Furan is a volatile compound (boiling temp. of 31.4 oC) formed during thermal processing of food. The toxicity of furan has been well documented and it is classified as “possible human carcinogen” (Group 2B) by the International Agency for Research on Cancer. Various pathways have been reported for furan formation during food processing. It can be formed from carbohydrates, amino acids by their thermal degradation or thermal re-arrangement and by oxidation of ascorbic acid and polyunsaturated acids and carotenoids. High concentrations of furan have been reported in coffee, baked and roasted food and in food subjected to preserving in cans and jars. Furan levels in brewed coffee are typically near or below 120 μg/L, but it can approach thousands μg/kg in roasted whole beans or ground coffee. The highest concentration of furan in roasted coffee reaches the level of 7000 μg/kg. Taking into account that coffee is the most popular hot drink, it becomes the main contributor to furan exposure from dietary sources for adults. In this article the published scientific papers concerned with the presence of furan in roasted non-brewed and brewed coffee have been reviewed. The formation mechanisms and occurrence of furan in coffee and the harmful influence of furan on the consumer health have been discussed.

  17. Evaluasi Kesesuaian Lahan Untuk Tanaman Kopi Arabika ( Coffea arabica ) di Dusun Paman Similir Desa Telagah Kecamatan Sel Bingei Kabupaten Langkat

    OpenAIRE

    Toruan, EIizabeth L.

    2013-01-01

    Land evaluation as the way to predict the land potention to many using. This analysis purpose to know the land appropriate in the village Pamah Similir distric of Sei Bingei regency on Langkat to the effectiveness of coffee plant until the sub class. This observation done at village Pamah Sirnilir district on Sei Bingei regency of Langkat. And begin from August 2004. This analysis begin from bibliografi research, collecting the data and completed the equipment that used in analysis, the...

  18. The coffee agroforestry system. Its importance for the agro-alimentary and nutritional security in Ecuador

    Directory of Open Access Journals (Sweden)

    Luciano Abelardo Ponce Vaca

    2018-01-01

    Full Text Available In the context of coffee growing, Ecuador is characterized by planting two main commercial species of Coffea arabica L. (Arabica coffee, and Coffea canephora Pierre ex Froehner (Robusta coffee, where small production units and agroforestry systems predominate. To overcome the low national production, which constitutes the central problem of coffee growing in the country, the Ministry of Agriculture and Livestock of Ecuador, promoted the project «Reactivation of the Ecuadorian coffee industry», which contributes to food security and nutrition with integral approach. In these circumstances, the objective of this paper is to analyze the importance of coffee agroforestry systems for agro-alimentary and nutritional security in Ecuador. The proposal is born from the agro-economic diagnosis «case study», from the situation of the Coffee Production Units, from the Association of Peasants «Juntos Lucharemos» from the La Unión parish of the Jipijapa county, province of Manabí, in which they settled Main contributions to the agro-alimentary and nutritional security coming from the coffee agroforestry systems, this allowed to analyze characteristics related to the object of study. Based on the research results, gaps in national consumption needs were detected. This proposal contributed to articulate and promote the reactivation of coffee growing on agroecological bases, in harmony with the dimensions of sustainability in order to protect and conserve biodiversity and the coffee forest.

  19. Concentração foliar de nutrientes em cultivares de Coffea arabica L. sob espaçamentos adensados Foliar nutrient contents in cultivars of Coffea arabica L. at condensed spacings

    Directory of Open Access Journals (Sweden)

    Humberto Silva Augusto

    2007-08-01

    Full Text Available Os plantios adensados de café proporcionam melhor reciclagem de nutrientes e conseqüentemente reduzem a perda de nutrientes do solo por erosão, lixiviação e oxidação da matéria orgânica. Avaliaram-se as influências de diferentes espaçamentos na concentração de macro e micronutrientes em folhas de cafeeiro das cultivares IAC 44, IAC 99, MG 1192, Katipó, MG 6851 e UFV 3880. Ao início do experimento o solo foi arado para incorporação do calcário. As mudas foram plantadas em covas de 0,30 x 0,30 x 0,40 m, espaçadas entre si em 0,75 m na fileira. Na adubação de plantio aplicou-se 200 g de Fosfato Natural de Araxá, 50 g de Superfosfato Simples por cova, e 15 dias após o plantio 2L/cova de esterco em cobertura. As demais adubações foram realizadas segundo o recomendado para a cultura do café. O delineamento foi em blocos casualizados num esquema fatorial 6 x 4 (seis cultivares x quatro espaçamentos, com seis repetições. As parcelas foram compostas por quatro fileiras de 4,50 m de comprimento, espaçadas entre fileiras em 1,0; 1,5; 2,0 e 2,5 m. Amostras de folhas foram retiradas aos 21 e 34 meses para avaliação dos teores foliares de macro e micronutrientes. O adensamento não influenciou na concentração de nutrientes nas folhas aos 21 meses após o plantio, mas influenciou as concentrações foliares de P, K e Mg aos 34 meses após o plantio.Coffee-tree condensed plantings provide reduction in the loss of the soil nutrients by erosion, lixiviation and oxidation of the organic matter, and even increase nutrient recycling. This experiment aimed evaluates the influence of different spacing among rows on the nutrient foliar contents of coffee-tree varieties, and was performed in randomized blocks with 24 treatments and six replications. The employed varieties IAC 44, IAC 99, MG 1192, Katipó, MG 6851 and UFV 3880 were planted with a spacing of 0,75 m among plants and 1,0; 1,5; 2,0 and 2,5m among rows. Each plot was composed

  20. Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica

    Directory of Open Access Journals (Sweden)

    Mirian Perez Maluf

    2009-01-01

    Full Text Available In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.

  1. Seed-specific stable expression of the alpha-AI1 inhibitor in coffee grains and the in vivo implications for the development of the coffee berry borer

    OpenAIRE

    Albuquerque, E. V. S.; Bezerra, C. A.; Romero, J. V.; Valencia, J. W. A.; Valencia-Jimenez, A.; Pimenta, L. M.; Barbosa, Aead; Silva, M. C. M.; Meneguim, A. M.; Sa, M. E. L.; Engler, G.; de Almeida-Engler, J.; Fernandez, Diana; Grossi-de-Sa, M. F.

    2015-01-01

    Genetic transformation of coffee (Coffea spp.), the second most traded commodity worldwide, is an alternative approach to introducing features that cannot be introgressed by traditional crossings. The transgenic stability, heritability and quantitative and spatial expression patterns of the seed-specific promoter phytohemagglutinin (PHA-L) from Phaseolus vulgaris were characterized in genetically modified C. arabica expressing the alpha-amylase inhibitor-1 (alpha-AI1) gene. The alpha-AI1 inhi...

  2. Seed-Specific Stable Expression of the ?-AI1 Inhibitor in Coffee Grains and the In Vivo Implications for the Development of the Coffee Berry Borer

    OpenAIRE

    Albuquerque, ?rika V. S.; Bezerra, Caroline A.; Romero, Juan V.; Valencia, Jorge W. A.; Valencia-Jim?nez, Arnubio; Pimenta, Lucas M.; Barbosa, Aulus E. A. D.; Silva, Maria C. M.; Meneguim, Ana M.; S?, Maria Eug?nia L.; Engler, Gilbert; de Almeida-Engler, Janice; Fernandez, Diana; Grossi-de-S?, Maria F.

    2015-01-01

    Genetic transformation of coffee (Coffea spp.), the second most traded commodity worldwide, is an alternative approach to introducing features that cannot be introgressed by traditional crossings. The transgenic stability, heritability and quantitative and spatial expression patterns of the seed-specific promoter phytohemagglutinin (PHA-L) from Phaseolus vulgaris were characterized in genetically modified C. arabica expressing the ?-amylase inhibitor-1 (?-AI1) gene. The ?-AI1 inhibitor shows ...

  3. Dynamic of Arabica Coffee Marketing Organization in Ngada District:Progress Upon Implementing of Geographical Indication

    OpenAIRE

    Aklimawati, Lya

    2017-01-01

    Farmer organization has important role on coffee agribusiness development. Organization was positioned as a driving force on farmer economic activities, especially in strengthening partnership networks. Realizing the importance of organization, the aim of this research was to identify the coffee market structure in the scheme of Geographical Indication; to analyze the dynamic of coffee marketing organization at farmers level; and to analyze added value of wet parchment bean sales at the farme...

  4. Quali- and quantitative analysis of commercial coffee by NMR

    International Nuclear Information System (INIS)

    Tavares, Leila Aley; Ferreira, Antonio Gilberto

    2006-01-01

    Coffee is one of the beverages most widely consumed in the world and the 'cafezinho' is normally prepared from a blend of roasted powder of two species, Coffea arabica and Coffea canephora. Each one exhibits differences in their taste and in the chemical composition, especially in the caffeine percentage. There are several procedures proposed in the literature for caffeine determination in different samples like soft drinks, coffee, medicines, etc but most of them need a sample workup which involves at least one step of purification. This work describes the quantitative analysis of caffeine using 1 H NMR and the identification of the major components in commercial coffee samples using 1D and 2D NMR techniques without any sample pre-treatment. (author)

  5. Climatic factors directly impact the volatile organic compound fingerprint in green Arabica coffee bean as well as coffee beverage quality.

    Science.gov (United States)

    Bertrand, B; Boulanger, R; Dussert, S; Ribeyre, F; Berthiot, L; Descroix, F; Joët, T

    2012-12-15

    Coffee grown at high elevations fetches a better price than that grown in lowland regions. This study was aimed at determining whether climatic conditions during bean development affected sensory perception of the coffee beverage and combinations of volatile compounds in green coffee. Green coffee samples from 16 plots representative of the broad range of climatic variations in Réunion Island were compared by sensory analysis. Volatiles were extracted by solid phase micro-extraction and the volatile compounds were analysed by GC-MS. The results revealed that, among the climatic factors, the mean air temperature during seed development greatly influenced the sensory profile. Positive quality attributes such as acidity, fruity character and flavour quality were correlated and typical of coffees produced at cool climates. Two volatile compounds (ethanal and acetone) were identified as indicators of these cool temperatures. Among detected volatiles, most of the alcohols, aldehydes, hydrocarbons and ketones appeared to be positively linked to elevated temperatures and high solar radiation, while the sensory profiles displayed major defects (i.e. green, earthy flavour). Two alcohols (butan-1,3-diol and butan-2,3-diol) were closely correlated with a reduction in aromatic quality, acidity and an increase in earthy and green flavours. We assumed that high temperatures induce accumulation of these compounds in green coffee, and would be detected as off-flavours, even after roasting. Climate change, which generally involves a substantial increase in average temperatures in mountainous tropical regions, could be expected to have a negative impact on coffee quality. Copyright © 2012 Elsevier Ltd. All rights reserved.

  6. Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation

    OpenAIRE

    Ribas, Alessandra Ferreira; Kobayashi, Adilson Kenji; Pereira, Luiz Filipe Protasio; Vieira, Luiz Gonzaga Esteves

    2006-01-01

    Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supp...

  7. Etude de l’hybridation inter-spécifique entre espèces du genre Coffea en Nouvelle-Calédonie : distribution des niches favorables et structuration de la diversité

    OpenAIRE

    Gomez , Céline

    2009-01-01

    Coffee trees are originated from inter-tropical forests of Africa and Madagascar and their diversification origin areas are highly threatened. Among the 103 species of the Coffea genus, only three are cultivated: C. arabica, C. canephora and C. liberica. C. canephora has the widest natural distribution in tropical African forests, and its genetic diversity is structured in five distinct groups. The study of the genetic diversity structuring has revealed a particular importance of the Dahomey ...

  8. Evaluation of the effect of roasting process on the energy transition and the crystalline structures of Arabica, Robusta, and Liberica coffee from Jambi Indonesia

    Science.gov (United States)

    Perdana, B. M.; Manihuruk, R.; Ashyar, R.; Heriyanti; Sutrisno

    2018-04-01

    The effect of the roasting process has been evaluated to determine of the energy transition and the crystalline structure of three types of coffee, Arabica, Robusta, and Liberica coffee both green and roasted coffee with the roasted temperature at 200°C and 230°C. The crystalline structure of the coffee was evaluated with X-ray powder diffraction (XRD). The result exposes that the three types of green coffee showed that an amorphous structure whereas the roasted coffee denotes a crystal structure of sucrose. The varied temperature in the roasting process leads to changes in the crystal structure shown by the peak shift of 2θ for all types of coffee. The added cations, such as Fe2+, Ca2+, and Mg2+ ions on Liberica coffee induced of changes in the crystal structures, which are assigned by the peak shift, that imply of metal ions of the sucrose complexes happened in the solution, except for the addition of Mg2+ ion.

  9. Estimation of Sensory Analysis Cupping Test Arabica Coffee Using NIR Spectroscopy

    Science.gov (United States)

    Safrizal; Sutrisno; Lilik, P. E. N.; Ahmad, U.; Samsudin

    2018-05-01

    Flavors have become the most important coffee quality parameters now day, many coffee consuming countries require certain taste scores for the coffee to be ordered, the currently used cupping method of appraisal is the method designed by The Specialty Coffee Association Of America (SCAA), from several previous studies was found that Near-Infrared Spectroscopy (NIRS) can be used to detect chemical composition of certain materials including those associated with flavor so it is possible also to be applied to coffee powder. The aim of this research is to get correlation between NIRS spectrum with cupping scoring by tester, then look at the possibility of testing coffee taste sensors using NIRS spectrum. The coffee samples were taken from various places, altitudes and postharvest handling methods, then the samples were prepared following the SCAA protocol, for sensory analysis was done in two ways, with the expert tester and with the NIRS test. The calibration between both found that Without pretreatment using PLS get RMSE cross validation 6.14, using Multiplicative Scatter Correction spectra obtained RMSE cross validation 5.43, the best RMSE cross-validation was 1.73 achieved by de-trending correction, NIRS can be used to predict the score of cupping.

  10. Evaluation of Somatic Embryogenesis Ability in Robusta Coffee (Coffea canephora Pierre

    Directory of Open Access Journals (Sweden)

    Priyono Priyono

    2010-08-01

    Full Text Available Embriogenesis somatik diharapkan sebagai metode perbanyakan tanaman yang sangat efektif pada kopi. Evaluasi dua jenis proses embriogenesis somatik, yaitu proses langsung dan tidak langsung akan bermanfaat untuk menggambarkan kemampuan proliferasi sel. Penelitian untuk mengevaluasi embriogenesis somatik kopi Robusta (Coffea canephora yang mempunyai tingkat keragaman genetik tinggi telah dilakukan di Nestlé R&D Centre Tours, Perancis. Bahan tanam menggunakan kopi Robusta koleksi Nestle Perancis dan tiga klon koleksi Pusat Penelitian Kopi dan Kakao Indonesia (Puslitkoka. Tiga aspek, yaitu proses embriogenesis, keragaman embriogenesis dan kemantapan embriogenesis dievaluasi dalam penelitian ini. Hasil penelitian menunjukkan bahwa baik embriogenesis somatik langsung maupun tidak langsung dapat diamati. Penelitian ini menunjukkan bahwa kedua proses embriogenesis somatik tersebut merupakan dua mekanisme yang berbeda. Dalam penelitian ini ditunjukkan bahwa kemampuan embriognesis somatik tergantung pada genotipe, baik antar maupun di dalam kelompok genetik kopi Robusta, yaitu Congolese,Guinean dan Conillon. Lebih lanjut diketahui bahwa kedua proses embriogenesis somatik tersebut stabil terhadap indukan sebagai sumber eksplan. Kemampuan embriogenesis somatik tidak langsung ketiga klon Puslitkoka (BP409, BP961 dan Q121 sangat beragam, sehingga memberikan harapan adanya pola segregasi yang baik berdasarkan kemampuan embriogenesis somatik tidak langsung pada populasi yang dibuat dari silangan klon tersebut.Key words: Coffea canephora, somatic embryogenesis, variability, stability, genotype.

  11. Efecto de micorriza vesiculo-arbuscular en café Coffea arabica L. variedad Colombia en almacigo

    Directory of Open Access Journals (Sweden)

    Sánchez de Prager Marina

    1990-06-01

    myriocarpa and desinfested soil. Coffee seedlings were grown on sterilized sand for two months, transplanted to plastic bags filled with soil and inoculated. The experimental design was complete randomized. Three plants from each treatment were harvested every two weeks during five months. Plant weight, dry matter, leaf area, root infection, spore counting and foliar analysis for N, P, K, Ca and Mg were recorded for each harvest. In the natural soil we found species of the genus Glomus, Entrophospora and Acaulospora. Eight days after transplanting we registered root infection by VAM. There were big differences among species. The greatest sporulation was found hundred days after transplanting G. manihotis was the best species as for as growth enhacement and nutrient uptake besides its influence on root development which was three fold compared with those on natural soil and desinfested soil treatments.

  12. Biogenic amine profile in unripe Arabica coffee beans processed according to dry and wet methods.

    Science.gov (United States)

    Dias, Eduardo C; Pereira, Rosemary G F A; Borém, Flávio M; Mendes, Eulália; de Lima, Renato R; Fernandes, José O; Casal, Susana

    2012-04-25

    Immature coffee fruit processing contributes to a high amount of defective beans, which determines a significant amount of low-quality coffee sold in the Brazilian internal market. Unripe bean processing was tested, taking the levels of bioactive amines as criteria for evaluating the extent of fermentation and establishing the differences between processing methods. The beans were processed by the dry method after being mechanically depulped immediately after harvest or after a 12 h resting period in a dry pile or immersed in water. Seven bioactive amines were quantified: putrescine, spermine, spermidine, serotonin, cadaverine, histamine, and tyramine, with global amounts ranging from 71.8 to 80.3 mg/kg. The levels of spermine and spermidine were lower in the unripe depulped coffee than in the natural coffee. The specific conditions of dry and wet processing also influenced cadaverine levels, and histamine was reduced in unripe depulped coffee. A resting period of 12 h does not induce significant alteration on the beans and can be improved if performed in water. These results confirm that peeling immature coffee can decrease fermentation processes while providing more uniform drying, thus reducing the number of defects and potentially increasing beverage quality.

  13. AMELIORATON GENETIQUE DE Coffea canephora Pierre PAR ...

    African Journals Online (AJOL)

    AISA

    Mots clés : Café, descendance, robusta, C. canephora, congusta, Côte d'Ivoire. ABSTRACT. GENETIC BREEDING OF coffea canephoraPierre BY INTERSPECIFIC HYBRIDIZATION : STUDY OF FIRST GENERATION. HYBRIDES OF coffea cannephoraPierre X coffea congensis Froenhner. Coffee represents one of the most ...

  14. Effect of altitude on biochemical composition and quality of green arabica coffee beans can be affected by shade and postharvest processing method.

    Science.gov (United States)

    Worku, Mohammed; de Meulenaer, Bruno; Duchateau, Luc; Boeckx, Pascal

    2018-03-01

    Although various studies have assessed altitude, shade and postharvest processing effects on biochemical content and quality of coffee beans, data on their interactions are scarce. The individual and interactive effects of these factors on the caffeine, chlorogenic acids (CGA) and sucrose contents as well as physical and sensory qualities of green coffee beans from large plantations in southwestern Ethiopia were evaluated. Caffeine and CGA contents decreased with increasing altitude; they respectively declined 0.12 and 1.23gkg -1 100m -1 . Sucrose content increased with altitude; however, the altitude effect was significant for wet-processed beans (3.02gkg -1 100m -1 ), but not for dry-processed beans (0.36g kg -1 100m -1 ). Similarly, sucrose content increased with altitude with much stronger effect for coffee grown without shade (2.11gkg -1 100m -1 ) compared to coffee grown under shade (0.93gkg -1 100m -1 ). Acidity increased with altitude when coffee was grown under shade (0.22 points 100m -1 ), but no significant altitude effect was observed on coffee grown without shade. Beans grown without shade showed a higher physical quality score for dry (37.2) than for wet processing (29.1). These results generally underline the complex interaction effects between altitude and shade or postharvest processing on biochemical composition and quality of green arabica coffee beans. Copyright © 2017. Published by Elsevier Ltd.

  15. Coffee Flower Honey (Coffea sp. Addition to Kefir Quality Based on Microbiology Characteristic

    Directory of Open Access Journals (Sweden)

    Astri Wulandari

    2017-11-01

    Full Text Available The Objective of this research was to determine the best concentration of addition coffee flower honey to kefir based on total plate count, lactic acid bacteria, acidity, and pH value. The materials used in this research were honey kefir made from cow’s milk, kefir grains, and coffee flowers honey. The method used in this research was experimental with Completely Randomized Design by 4 treatments and 4 replications and continued by using Duncan’s Multiple Range Test. The result showed that the effect of addition coffee flowers honey was not significantly different (P>0.05 on total plate count, lactic acid bacteria, acidity, give highly significant different (P<0.01 on pH value. Addition 20% of  coffee flower honey was the best treatment with quality of total plate count 6.21 ± 0.60 log CFU/mL, total lactic acid bacteria 6.16 ± 0.14 log CFU/mL, acidity 0.76 ± 0.11 % and pH value 3.596 ± 0.021. Further study is needed to figure the storability of honey kefir.

  16. Characterization and dynamic of colonization of Cladosporium cladosporioides (Fresen.) de Vries in coffee fruits (Coffea arabica L.)

    OpenAIRE

    Pereira, Ricardo Tadeu Galvão; Pfenning, Ludwig Heinrich; Castro, Hilário Antônio de

    2005-01-01

    A presença de fungos em associação natural com frutos do cafeeiro é considerada um fator importante influenciando a qualidade do café. A influência negativa de algumas espécies de Aspergillus é conhecida, comprometendo inclusive a segurança do produto. Os relatos de fungos influenciando positivamente a qualidade se resumem à ocorrência de Cladosporium sp. associados a grãos que originaram cafés de boa qualidade, porém informações exatas sobre a espécie e a sua dinâmica no campo são escassas. ...

  17. Microwave-assisted extraction of green coffee oil and quantification of diterpenes by HPLC.

    Science.gov (United States)

    Tsukui, A; Santos Júnior, H M; Oigman, S S; de Souza, R O M A; Bizzo, H R; Rezende, C M

    2014-12-01

    The microwave-assisted extraction (MAE) of 13 different green coffee beans (Coffea arabica L.) was compared to Soxhlet extraction for oil obtention. The full factorial design applied to the microwave-assisted extraction (MAE), related to time and temperature parameters, allowed to develop a powerful fast and smooth methodology (10 min at 45°C) compared to a 4h Soxhlet extraction. The quantification of cafestol and kahweol diterpenes present in the coffee oil was monitored by HPLC/UV and showed satisfactory linearity (R(2)=0.9979), precision (CV 3.7%), recovery (yield calculated on the diterpenes content for sample AT1 (Arabica green coffee) showed a six times higher value compared to the traditional Soxhlet method. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. GENETIC DIVERSITY IN ARABICA COFFEE GROWN IN POTASSIUM-CONSTRAINED ENVIRONMENT

    Directory of Open Access Journals (Sweden)

    Waldênia de Melo Moura

    2015-02-01

    Full Text Available Potassium is a source of non-renewable natural resource, and is used in large quantities in coffee fertilization through basically imported formulations in the form of potassium chloride. An alternative to make production systems more sustainable would be obtaining cultivars more efficient in the use of this nutrient. This study aimed to evaluate the genetic diversity among 20 cultivars of coffee, in conditions of low availability of potassium to identify the best combinations for composing future populations to be used in breeding programs. The experiment was arranged in a randomized block design with three replications of nutrient solution. Agronomic characteristics and efficiencies of rooting, absorption, translocation, biomass production and potassium utilization were evaluated. The clustering analysis was based on the unweighted pair group method with arithmetic mean clustering algorithm (UPGMA and canonical variables. Variability was observed for most treatments. The multivariate procedures produced similar discrimination of genotypes, with the formation of five groups. Hybridizations between the cultivar Icatu Precoce IAC 3283 with cultivars Catuaí Amarelo IAC 62, Araponga MG1, Caturra Vermelho IAC 477, Catuaí Vermelho IAC 15, Rubi MG 1192 and Catucaí 785/15, and between the cultivar Tupi IAC 1669-33 with cultivars Icatu Vermelho IAC 4045, Acaiá Cerrado MG 1474 and Oeiras MG 6851 are the most promising for obtaining segregating populations or heterotic hybrids in breeding programs aiming more efficiency in potassium utilization.

  19. Agrometeorological parameters for prediction of the maturation period of Arabica coffee cultivars

    Science.gov (United States)

    Pezzopane, José Ricardo Macedo; Salva, Terezinha de Jesus Garcia; de Lima, Valéria Bittencourt; Fazuoli, Luiz Carlos

    2012-09-01

    The objective of this study was to determine the harvest period of coffee fruits based on the relationship between agrometeorological parameters and sucrose accumulation in the seeds. Over the crop years 2004/2005 and 2006/2007, from 150 days after flowering (DAF) onwards, samples of 50 fruits of cultivars Mundo Novo IAC 376-4, Obatã IAC 1669-20 and Catuaí Vermelho IAC 144 were collected from coffee trees located in Campinas, Brazil. The endosperm of the fruits was freeze-dried, ground and analyzed for sucrose content by high-performance liquid chromatography. A weather station provided data to calculate the accumulated growing degree-day (GDD) units, and the reference (ETo) and actual (ETr) evapotranspiration rates. The results showed that the highest rates of sucrose accumulation occurred at the transition from the cane-green to the cherry phenological stage. Models for the estimation of sucrose content during maturation based on meteorological variables exhibited similar or better performance than the DAF variable, with better results for the variables GDD and ETo. The Mundo Novo cultivar reached the highest sucrose level in the endosperm after 2,790 GDD, while cultivar Catuaí attained its maximum sucrose concentration after the accumulated evapotranspiration rate has reached a value of 870 mm. As for cultivar Obatã, the maximum sucrose concentration was predicted with the same degree of accuracy using any of the parameters investigated. For the Obatã cultivar, the values of the variables calculated for the maximum sucrose concentration to be reached were 249 DAF, 3,090 GDD, 1,020 ETo and 900 ETr.

  20. Characterization of Fatty Acid, Amino Acid and Volatile Compound Compositions and Bioactive Components of Seven Coffee (Coffea robusta Cultivars Grown in Hainan Province, China

    Directory of Open Access Journals (Sweden)

    Wenjiang Dong

    2015-09-01

    Full Text Available Compositions of fatty acid, amino acids, and volatile compound were investigated in green coffee beans of seven cultivars of Coffea robusta grown in Hainan Province, China. The chlorogenic acids, trigonelline, caffeine, total lipid, and total protein contents as well as color parameters were measured. Chemometric techniques, principal component analysis (PCA, hierarchical cluster analysis (HCA, and analysis of one-way variance (ANOVA were performed on the complete data set to reveal chemical differences among all cultivars and identify markers characteristic of a particular botanical origin of the coffee. The major fatty acids of coffee were linoleic acid, palmitic acid, oleic acid, and arachic acid. Leucine (0.84 g/100 g DW, lysine (0.63 g/100 g DW, and arginine (0.61 g/100 g DW were the predominant essential amino acids (EAAs in the coffee samples. Seventy-nine volatile compounds were identified and semi-quantified by HS-SPME/GC-MS. PCA of the complete data matrix demonstrated that there were significant differences among all cultivars, HCA supported the results of PCA and achieved a satisfactory classification performance.

  1. Vegetation classification of Coffea on Hawaii Island using WorldView-2 satellite imagery

    Science.gov (United States)

    Gaertner, Julie; Genovese, Vanessa Brooks; Potter, Christopher; Sewake, Kelvin; Manoukis, Nicholas C.

    2017-10-01

    Coffee is an important crop in tropical regions of the world; about 125 million people depend on coffee agriculture for their livelihoods. Understanding the spatial extent of coffee fields is useful for management and control of coffee pests such as Hypothenemus hampei and other pests that use coffee fruit as a host for immature stages such as the Mediterranean fruit fly, for economic planning, and for following changes in coffee agroecosystems over time. We present two methods for detecting Coffea arabica fields using remote sensing and geospatial technologies on WorldView-2 high-resolution spectral data of the Kona region of Hawaii Island. The first method, a pixel-based method using a maximum likelihood algorithm, attained 72% producer accuracy and 69% user accuracy (68% overall accuracy) based on analysis of 104 ground truth testing polygons. The second method, an object-based image analysis (OBIA) method, considered both spectral and textural information and improved accuracy, resulting in 76% producer accuracy and 94% user accuracy (81% overall accuracy) for the same testing areas. We conclude that the OBIA method is useful for detecting coffee fields grown in the open and use it to estimate the distribution of about 1050 hectares under coffee agriculture in the Kona region in 2012.

  2. Impact of climate change on agro-climatic zoning of Arabica coffee in the State of Espirito Santo, Brazil = Impactos das mudanças climáticas no zoneamento agroclimatológico do café arábica no Espírito Santo

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    Rosembergue Bragança

    2016-06-01

    Full Text Available The aim of this study was to define, using current agro-climatic zoning, and for the next 100 years, areas of different climatic suitability for the cultivation of Arabica coffee (Coffea arabica L. in the State of Espirito Santo, Brazil. Monthly and yearly data of average air temperature and rainfall were used, taken from historical series for the period of 1976 to 2006. It was necessary to simulate the effects of temperature increments of +1 ºC, +2 ºC, +3 ºC, +4 ºC and +5 ºC, using the mean result of six models, namely: GFDL-R30 (Geophysical Fluid Dynamics Laboratory, R-30 resolution, CCSR/NIES (Center for Climate Research Studies, CSIROMk2 (Commonwealth Scientific and Industrial Research Organization GCM mark 2, CGCM2 (Canadian Global Coupled Model v 2, ECHAM4 (European Centre Hamburg Model v 4 and HadCM3 (Hadley Centre Coupled Model v 3. The results showed that currently, areas which are completely suitable represent 19.49% of the area of Espirito Santo which, with an increase of 5 °C, would decrease to 0.02%; whereas completely unsuitable areas would increase from 33.47% to 95.63%, making the cultivation of Arabica coffee unsuited to the state if the genetic and physiological characteristics, which have a tolerance limit for the average annual temperature of between 23 °C and 24 °C, are maintained. = Objetivou-se com este trabalho definir, por meio do zoneamento agroclimatológico atual e para os próximos 100 anos, áreas com diferentes aptidões climáticas para a cultura do café arábica (Coffea arabica L., no estado do Espírito Santo. Para isso, foram utilizados dados de temperatura média do ar e precipitação pluviométrica, em escala mensal e anual, de séries históricas representativas do período de 1976 a 2006. Foi necessário simular o efeito do incremento de temperatura de +1 ºC, +2 ºC, +3 ºC, +4 ºC e +5 ºC, por meio da média obtida do resultado de seis modelos, a saber: GFDL-R30 (Geophysical Fluid Dynamics

  3. Evaluación de la incidencia de la aplicación foliar de ácido giberélico en la floración de árboles de coffea arabica l. y su impacto frente al cambio climático

    OpenAIRE

    Zapata Restrepo, Leidy Natalia

    2013-01-01

    Tesis (Maestría en Desarrollo Sostenible y Medio Ambiente). Universidad de Manizales. Facultad de Ciencias Contables, Económicas y Administrativas, 2013 La giberelina es conocida como una fitohormona que participa activamente en el control de la floración de varias especies. En el presente estudio se evaluó la aplicación exógena de ácido giberélico (0.1% y 0.5%) a 2959 plantas de Coffea arabica L., variedad Castillo cultivar Rosario con el Objetivo de establecer la relación existente entr...

  4. Looking into individual coffee beans during the roasting process: direct micro-probe sampling on-line photo-ionisation mass spectrometric analysis of coffee roasting gases.

    Science.gov (United States)

    Hertz-Schünemann, Romy; Streibel, Thorsten; Ehlert, Sven; Zimmermann, Ralf

    2013-09-01

    A micro-probe (μ-probe) gas sampling device for on-line analysis of gases evolving in confined, small objects by single-photon ionisation time-of-flight mass spectrometry (SPI-TOFMS) was developed. The technique is applied for the first time in a feasibility study to record the formation of volatile and flavour compounds during the roasting process within (inside) or in the direct vicinity (outside) of individual coffee beans. A real-time on-line analysis of evolving volatile and semi-volatile organic compounds (VOC and SVOC) as they are formed under the mild pyrolytic conditions of the roasting process was performed. The soft-ionisation mass spectra depict a molecular ion signature, which is well corresponding with the existing knowledge of coffee roasting and evolving compounds. Additionally, thereby it is possible to discriminate between Coffea arabica (Arabica) and Coffea canephora (Robusta). The recognized differences in the roasting gas profiles reflect the differences in the precursor composition of the coffee cultivars very well. Furthermore, a well-known set of marker compounds for Arabica and Robusta, namely the lipids kahweol and cafestol (detected in their dehydrated form at m/z 296 and m/z 298, respectively) were observed. If the variation in time of different compounds is observed, distinctly different evolution behaviours were detected. Here, phenol (m/z 94) and caffeine (m/z 194) are exemplary chosen, whereas phenol shows very sharp emission peaks, caffeine do not have this highly transient behaviour. Finally, the changes of the chemical signature as a function of the roasting time, the influence of sampling position (inside, outside) and cultivar (Arabica, Robusta) is investigated by multivariate statistics (PCA). In summary, this pilot study demonstrates the high potential of the measurement technique to enhance the fundamental knowledge of the formation processes of volatile and semi-volatile flavour compounds inside the individual coffee bean.

  5. Genotyping-by-sequencing provides the first well-resolved phylogeny for coffee (Coffea) and insights into the evolution of caffeine content in its species: GBS coffee phylogeny and the evolution of caffeine content.

    Science.gov (United States)

    Hamon, Perla; Grover, Corrinne E; Davis, Aaron P; Rakotomalala, Jean-Jacques; Raharimalala, Nathalie E; Albert, Victor A; Sreenath, Hosahalli L; Stoffelen, Piet; Mitchell, Sharon E; Couturon, Emmanuel; Hamon, Serge; de Kochko, Alexandre; Crouzillat, Dominique; Rigoreau, Michel; Sumirat, Ucu; Akaffou, Sélastique; Guyot, Romain

    2017-04-01

    A comprehensive and meaningful phylogenetic hypothesis for the commercially important coffee genus (Coffea) has long been a key objective for coffee researchers. For molecular studies, progress has been limited by low levels of sequence divergence, leading to insufficient topological resolution and statistical support in phylogenetic trees, particularly for the major lineages and for the numerous species occurring in Madagascar. We report here the first almost fully resolved, broadly sampled phylogenetic hypothesis for coffee, the result of combining genotyping-by-sequencing (GBS) technology with a newly developed, lab-based workflow to integrate short read next-generation sequencing for low numbers of additional samples. Biogeographic patterns indicate either Africa or Asia (or possibly the Arabian Peninsula) as the most likely ancestral locality for the origin of the coffee genus, with independent radiations across Africa, Asia, and the Western Indian Ocean Islands (including Madagascar and Mauritius). The evolution of caffeine, an important trait for commerce and society, was evaluated in light of our phylogeny. High and consistent caffeine content is found only in species from the equatorial, fully humid environments of West and Central Africa, possibly as an adaptive response to increased levels of pest predation. Moderate caffeine production, however, evolved at least one additional time recently (between 2 and 4Mya) in a Madagascan lineage, which suggests that either the biosynthetic pathway was already in place during the early evolutionary history of coffee, or that caffeine synthesis within the genus is subject to convergent evolution, as is also the case for caffeine synthesis in coffee versus tea and chocolate. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. Collimated scanning LS-INAA for testing trace elements homogeneity in Brazilian coffee beans

    International Nuclear Information System (INIS)

    Tagliaferro, F.S.; Nadai Fernandes de, E.A.; Bode, P.; Baas, H.W.

    2008-01-01

    The degree of homogeneity is normally assessed by the variability of the results of independent analyses of several (e.g., 15) normal-scale replicates. Large sample instrumental neutron activation analysis (LS-INAA) with a collimated Ge detector allows inspecting the degree of homogeneity of the initial batch material, using a kilogram-size sample. The test is based on the spatial distributions of induced radioactivity. Such test was applied to samples of Brazilian whole (green) coffee beans (Coffea arabica and Coffea canephora) of approximately 1 kg in the frame of development of a coffee reference material. Results indicated that the material do not contain significant element composition inhomogeneities between batches of approximately 30-50 g, masses typically forming the starting base of a reference material. (author)

  7. Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan Somatic Embryogenesis (The effects of shooting and rooting of arabica coffee propagation through Embryogenesis Somatic auxin uses.

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    Rina Arimarsetiowati

    2012-08-01

    Full Text Available Plantlet that has developed shoots and roots will have a high level adaptation in the field. The objective of this experiment was to improve the ability of planlet in shooting and rooting so that it is ready for acclimatization in the field. The increase ability in shooting and rooting of the planlet were conducted by adding various types of auxin in the media. The arabica coffee embryo of clone AS 2K which has entered the phase of the cotyledons was transfered into the treatment media containing half-strength of MS (Murashige & Skoog macro and micro nutrient, vitamin B5, 30 g/L glucose, 100 ml/L coconut water, 50 mg/L AgNO3 added with the combination of IAA, IBA and NAA. The research was conducted by using completely randomized design with seven combined treatment i.e. 0.1 mg/L IBA, 0.1 mg/L NAA, 0.1 mg/L IAA; 0 , 1 mg/L IBA + 0.1 mg/L NAA, 0.1 mg/L IBA + 0.1 mg/L IAA, 0.1 mg/L NAA + 0.1 mg/L IAA; without auxin. There were 12 replications in every treatment and each replication consisted of five cotyledonary embryos. The parameters of observation were the root length, leaf number, leaf area, stem diameter, and height of plantlets. The observations were conducted in eighth weeks after cotyledonary embryo had shoots. The results showed that in the number of leaves and height of planlet parameters, the treatment without auxin was the best result compared to planlet with auxin addition. The addition of auxin varians and their combination did not significantly influent leaf area, root length and stem diameter parameters. The medium tested was optimum for the growth of shoots and roots of AS 2K arabica coffee.

  8. Cinética da secagem de clones de café (Coffea canephora Pierre em terreiro de chão batido Kinetics of coffee berry clones drying (Coffea canephora Pierre in ground pavement

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    Osvaldo Resende

    2010-01-01

    Full Text Available Objetivou-se com o presente trabalho estudar a cinética da secagem de quatro clones de café da espécie Coffea canephora submetidos à secagem em terreiro de chão batido, bem como ajustar diferentes modelos matemáticos aos valores experimentais selecionando aquele que melhor representa o fenômeno em estudo. Foram utilizados frutos de café dos clones: Cpafro 194, Cpafro 193, Cpafro 167 e Cpafro180, colhidos com os teores de água iniciais de 1,20; 1,32; 1,51 e 1,46 (decimal base seca (b.s., respectivamente. A secagem prosseguiu em terreiro de chão batido até que o produto atingisse o teor de água de 0,137; 0,133; 0,142 e 0,140 (decimal b.s. respectivamente para os clones Cpafro 194, Cpafro 193, Cpafro 167 e Cpafro 180. Aos dados experimentais foram ajustados dez modelos matemáticos citados na literatura específica e utilizados para representação do processo de secagem de produtos agrícolas. Baseando-se em parâmetros estatísticos, conclui-se que os modelos Verma, Dois Termos e Aproximação da Difusão foram adequados para representação da secagem dos quatro clones de café analisados, e além destes, para o clone Cpafro 167, os modelos Thompson, Page, Newton, Logarítmico, Henderson e Pabis e Exponencial de Dois Termos também se mostraram satisfatórios na descrição do fenômeno; já o tempo necessário para a secagem em terreiro de chão batido dos clones de café Cpafro 194, Cpafro 193, Cpafro 167 e Cpafro 180 foi de 189,5 h.The objective of this work was to study the drying kinetics of four coffee berry clones Coffea canephora species dried in ground pavement and to fit different mathematical models to different experimental data selecting the one that best represents the phenomenon. Have been used coffee berry of clones: Cpafro 194, Cpafro 193, Cpafro 167 and Cpafro 180, harvested at moisture content of 1.20; 1.32; 1.51 e 1.46 (decimal d.b., respectively. The drying continued in ground pavement until achieved the moisture

  9. IRRIGATED ARABICA COFFEE TREE PRODUCTIVITY IN THE CERRADO AREA OF THE GOIÁS STATE, BRAZIL PRODUTIVIDADE DE CAFEEIROS ARÁBICA IRRIGADOS NO CERRADO GOIANO

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    Américo Nunes Silveira Neto

    2008-11-01

    Full Text Available

    The aim of this work was to evaluate irrigation effects on the productivity and income of coffee cultivars (Catuaí IAC 44, Acaiá Cerrado MG 1474, Rubi MG 1192, Topázio MG 1190, Oeiras MG 6851, and Katipó, in order to identify the potentiality for the irrigated coffee production in the Cerrado area, in the southwest region of the Goiás State. The coffee cultivars, planted with 3.50 m between rows, and 0.50 m between plants in the row, were evaluated without irrigation and irrigated with sprinkling and drip systems. The trial was conducted, from 2002 to 2006, at the Universidade Federal de Goiás, Jataí Campus, in the Goiás State, concentrating irrigation from May to September. The irrigation water management was controlled through soil water balance. The irrigation process doubled the productivity and decreased the processing yield rate, while no significant difference was found among the irrigation methods. The Katipó cultivar showed a significantly higher productivity rate, with a good potential to be cultivated on the region and no influence of the water supply system, i.e., without irrigation, sprinkle irrigation, or drip irrigation. The cultivar Acaiá Cerrado MG 1474 featured the lowest productivity rate.

    KEY-WORDS: Coffea arabica; irrigated coffee; drip irrigation;  sprinkle irrigation.

     

  1. Biochemical and molecular characterization of the antioxidative system of Coffea sp. under cold conditions in genotypes with contrasting tolerance.

    Science.gov (United States)

    Fortunato, Ana S; Lidon, Fernando C; Batista-Santos, Paula; Leitão, António Eduardo; Pais, Isabel P; Ribeiro, Ana I; Ramalho, José Cochicho

    2010-03-15

    Low positive temperature (chilling) is frequently linked to the promotion of oxidative stress conditions, and is of particular importance in the coffee plant due to its severe impact on growth, development, photosynthesis and production. Nevertheless, some acclimation ability has been reported within the Coffea genus, and is possibly related to oxidative stress control. Using an integrated biochemical and molecular approach, the characterization of the antioxidative system of genotypes with different cold acclimation abilities was performed. Experiments were carried out using 1.5-year-old coffee seedlings of Coffea canephora cv. Apoatã, C. arabica cv. Catuaí, C. dewevrei and 2 hybrids, Icatu (C. arabicaxC. canephora) and Piatã (C. dewevreixC. arabica) subjected to a gradual cold treatment and a recovery period. Icatu showed the greatest ability to control oxidative stress, as reflected by the enhancement of several antioxidative components (Cu,Zn-SOD and APX activities; ascorbate, alpha-tocopherol and chlorogenic acids (CGAs) contents) and lower reactive oxygen species contents (H(2)O(2) and OH). Gene expression studies show that GRed, DHAR and class III and IV chitinases might also be involved in the cold acclimation ability of Icatu. Catuaí showed intermediate acclimation ability through the reinforcement of some antioxidative molecules, usually to a lesser extent than that observed in Icatu. On the other hand, C. dewevrei showed the poorest response in terms of antioxidant accumulation, and also showed the greatest increase in OH values. The difference in the triggering of antioxidative traits supports the hypothesis of its importance to cold (and photoinhibition) tolerance in Coffea sp. and could provide a useful probe to identify tolerant genotypes. Copyright 2009 Elsevier GmbH. All rights reserved.

  2. Modelagem matemática e determinação das propriedades termodinâmicas do café (Coffea arabica L. durante o processo de secagem

    Directory of Open Access Journals (Sweden)

    Paulo Cesar Corrêa

    2010-10-01

    Full Text Available A secagem de produtos agrícolas é largamente utilizada no mundo para o controle e a manutenção da qualidade dos produtos agrícolas. O objetivo do presente trabalho foi modelar o processo de secagem e obter os parâmetros termodinâmicos de frutos de café (Coffea Arabica L., cultivar Catuaí Amarelo, para três diferentes condições de temperatura e umidade relativa (35 ºC e 32,1%; 45 ºC e 15,7%; e 55 ºC e 10,2%. Foram utilizados frutos de café colhidos manualmente com teor inicial de água de 1,25 (b.s. e submetidos à secagem até atingirem o teor médio de 0,13 (b.s. Seis modelos matemáticos usualmente utilizados para a representação do processo de secagem de produtos agrícolas foram ajustados aos dados experimentais. A segunda lei de Fick foi utilizada para obter os coeficientes de difusão dos frutos de café por meio da cinética da secagem. A energia de ativação para a secagem dos frutos de café, bem como a entropia, entalpia e energia livre de Gibbs, foram obtidas. O modelo de Midili modificado foi o que melhor representou o fenômeno de secagem de frutos de café. Os valores do coeficiente de difusão obtidos foram 2,99 x 10-11, 2,39 x 10-11 e 5,98 x 10-11 m² s-1 para as temperaturas de 35, 45 e 55 ºC, respectivamente. A entalpia diminuiu com o aumento da temperatura do ar de secagem, bem como a entropia. A energia livre de Gibbs aumentou com o aumento da temperatura.

  3. Leaf-associated bacterial microbiota of coffee and its correlation with manganese and calcium levels on leaves

    Directory of Open Access Journals (Sweden)

    Leandro Pio de Sousa

    2018-05-01

    Full Text Available Abstract Coffee is one of the most valuable agricultural commodities and the plants’ leaves are the primary site of infection for most coffee diseases, such as the devastating coffee leaf rust. Therefore, the use of bacterial microbiota that inhabits coffee leaves to fight infections could be an alternative agricultural method to protect against coffee diseases. Here, we report the leaf-associated bacteria in three coffee genotypes over the course of a year, with the aim to determine the diversity of bacterial microbiota. The results indicate a prevalence of Enterobacteriales in Coffea canephora, Pseudomonadales in C. arabica ‘Obatã’, and an intriguing lack of bacterial dominance in C. arabica ‘Catuaí’. Using PERMANOVA analyses, we assessed the association between bacterial abundance in the coffee genotypes and environmental parameters such as temperature, precipitation, and mineral nutrients in the leaves. We detected a close relationship between the amount of Mn and the abundance of Pseudomonadales in ‘Obatã’ and the amount of Ca and the abundance of Enterobacteriales in C. canephora. We suggest that mineral nutrients can be key drivers that shape leaf microbial communities.

  4. Leaf-associated bacterial microbiota of coffee and its correlation with manganese and calcium levels on leaves.

    Science.gov (United States)

    de Sousa, Leandro Pio de; da Silva, Marcio José da; Mondego, Jorge Maurício

    2018-05-17

    Coffee is one of the most valuable agricultural commodities and the plants' leaves are the primary site of infection for most coffee diseases, such as the devastating coffee leaf rust. Therefore, the use of bacterial microbiota that inhabits coffee leaves to fight infections could be an alternative agricultural method to protect against coffee diseases. Here, we report the leaf-associated bacteria in three coffee genotypes over the course of a year, with the aim to determine the diversity of bacterial microbiota. The results indicate a prevalence of Enterobacteriales in Coffea canephora, Pseudomonadales in C. arabica 'Obatã', and an intriguing lack of bacterial dominance in C. arabica 'Catuaí'. Using PERMANOVA analyses, we assessed the association between bacterial abundance in the coffee genotypes and environmental parameters such as temperature, precipitation, and mineral nutrients in the leaves. We detected a close relationship between the amount of Mn and the abundance of Pseudomonadales in 'Obatã' and the amount of Ca and the abundance of Enterobacteriales in C. canephora. We suggest that mineral nutrients can be key drivers that shape leaf microbial communities.

  5. Avaliação do teor de cafeína em folhas e grãos de acessos de café arábica Evaluation of the caffeine content in leaves and grains of arabica coffee accessions

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    Alexsandro Lara Teixeira

    2012-03-01

    Full Text Available O presente trabalho teve como objetivo identificar, dentro do banco de germoplasma do estado de Minas Gerais, acessos de Coffea arabica L. com baixos teores de cafeína e verificar a existência de correlação entre o teor de cafeína dos grãos e de folhas ainda no estádio de mudas, viabilizando a prática da seleção precoce. Foram utilizados 75 acessos de café (cultivares, híbridos e alguns genótipos selvagens oriundos do banco de germoplasma de café do Estado de Minas Gerais. Para estudo da correlação foram utilizados oito cultivares no delineamento de blocos casualizados com três repetições. Avaliaram-se os teores de cafeína presentes nos grãos e no terceiro e quarto par de folhas verdadeiras. Seis acessos apresentaram teores de cafeína nos grãos menores que 0,88%. Correlações significativas para teor de cafeína foram observadas entre o terceiro (0,69 e quarto par de folhas (0,92 e os grãos. Foram identificados acessos com teores reduzidos de cafeína e boa produtividade de grãos, podendo ser utilizados como genitores em programas de melhoramento. Verificou-se que é possível realizar a seleção precoce para teor de cafeína, em plantas de cafeeiro ainda no estádio de mudas, por meio da avaliação do quarto par de folhas.The aim of this study was to identify, within the germplasm bank of the Minas Gerais state, Coffea arabica L. accessions with low levels of caffeine and check the correlation between grain and leaves in seedling stage, making possible the practice of early selection. Seventy-five coffe accessions (cultivars, hybrids and several wild genotypes were evaluated from the coffee germplasm bank of the Minas Gerais state. In the correlation study, eight cultivars were used on randomized complete block design with three replications. Were evaluated the caffeine levels in the grains and the third and fourth pair of true leaves. Six accessions had caffeine levels in grains smaller than 0.88%. Was also detected

  6. PERBANDINGAN KARAKTERISTIK KIMIA DAN NILAI SENSORI ANTARA KOPI LUWAK DAN KOPI BIASA DARI VARIETAS ARABICA (Cafeea arabica. L) DAN ROBUSTA (Cafeea canephora. L)

    OpenAIRE

    Mahendradatta, Meta; Zainal; Israyanti; Abu Bakar, Tawali

    2012-01-01

    "Luwak??? coffee is well known as an extraordinary coffee due to its taste and high sell price. Special taste and odor of ???luwak??? coffee are caused by the change of protein, fat and caffeine content. This research aimed to know the comparison of caffein content, proximate analysis (protein and fat), taste and odor between ???luwak??? coffee and original coffee from arabika (Caffea arabica L) and robusta (Caffea canephora L) varieties. The treatments were robusta ???luwak???, arabica ???lu...

  7. Soil phosphorus dynamics and availability and irrigated coffee yield

    Directory of Open Access Journals (Sweden)

    Thiago Henrique Pereira Reis

    2011-04-01

    Full Text Available Research data have demonstrated that the P demand of coffee (Coffea arabica L. is similar to that of short-cycle crops. In this context, the objective of this study was to evaluate the influence of annual P fertilization on the soil P status by the quantification of labile, moderately labile, low-labile, and total P fractions, associating them to coffee yield. The experiment was installed in a typical dystrophic Red Latosol (Oxisol cultivated with irrigated coffee annually fertilized with triple superphosphate at rates of 0, 50, 100, 200, and 400 kg ha-1 P2O5. Phosphorus fractions were determined in two soil layers: 0-10 and 10-20 cm. The P leaf contents and coffee yield in 2008 were also evaluated. The irrigated coffee responded to phosphate fertilization in the production phase with gains of up to 138 % in coffee yield by the application of 400 kg ha-1 P2O5. Coffee leaf P contents increased with P applications and stabilized around 1.98 g kg-1, at rates of 270 kg ha-1 P2O5 and higher. Soil P application caused, in general, an increase in bioavailable P fractions, which constitute the main soil P reservoir.

  8. Development of an instant coffee enriched with chlorogenic acids.

    Science.gov (United States)

    Corso, Marinês Paula; Vignoli, Josiane Alessandra; Benassi, Marta de Toledo

    2016-03-01

    The objective of this study was to present possible formulations for an instant coffee product enriched with chlorogenic acids for the Brazilian market. Formulations were prepared with different concentrations of freeze dried extracts of green Coffea canephora beans (G) added to freeze dried extracts of roasted Coffea arabica (A) and Coffea canephora (C). Medium (M) and dark (D) roasting degrees instant coffee were produced (AM, AD, CM and CD) to obtain four formulations with green extract addition (AMG, ADG, CMG and CDG). Chlorogenic acids were determined by HPLC, with average contents of 7.2 %. Roasted extracts and formulations were evaluated for 5-CQA and caffeine contents (by HPLC), browned compounds (absorbance 420 nm), and antioxidant activity (ABTS and Folin). Coffee brews of the four formulations were also assessed in a lab-scale test by 42 consumers for acceptance of the color, aroma, flavor and body, overall acceptance and purchase intent, using a 10 cm hybrid scale. The formulations obtained acceptance scores of 6.6 and 7.7 for all attributes, thus they were equally acceptable. Greater purchase intent was observed for ADG, CDG and CMG (6.9) in comparison to AMG (6.1). The formulations had, on average, 2.5 times more 5-CQA than the average obtained from conventional commercial instant coffees. In addition to being more economically viable, the formulations developed with C. canephora (CDG and CMG) showed greater antioxidant potential (32.5 g of Trolox/100 g and 13.8 g of gallic acid equivalent/100 g) due to a balance in the amount of bioactive compounds.

  9. Characterization of Coffee ringspot virus-Lavras: A model for an emerging threat to coffee production and quality

    Energy Technology Data Exchange (ETDEWEB)

    Ramalho, T.O.; Figueira, A.R.; Sotero, A.J. [Universidade Federal de Lavras, Departamento de Fitopatologia, Caixa Postal 3037, CEP 37200-000 Lavras, MG (Brazil); Wang, R. [Department of Plant Pathology, University of Kentucky, Lexington, KY 40546 (United States); Geraldino Duarte, P.S. [Universidade Federal de Lavras, Departamento de Fitopatologia, Caixa Postal 3037, CEP 37200-000 Lavras, MG (Brazil); Farman, M. [Department of Plant Pathology, University of Kentucky, Lexington, KY 40546 (United States); Goodin, M.M., E-mail: mgoodin@uky.edu [Department of Plant Pathology, University of Kentucky, Lexington, KY 40546 (United States)

    2014-09-15

    The emergence of viruses in Coffee (Coffea arabica and Coffea canephora), the most widely traded agricultural commodity in the world, is of critical concern. The RNA1 (6552 nt) of Coffee ringspot virus is organized into five open reading frames (ORFs) capable of encoding the viral nucleocapsid (ORF1p), phosphoprotein (ORF2p), putative cell-to-cell movement protein (ORF3p), matrix protein (ORF4p) and glycoprotein (ORF5p). Each ORF is separated by a conserved intergenic junction. RNA2 (5945 nt), which completes the bipartite genome, encodes a single protein (ORF6p) with homology to RNA-dependent RNA polymerases. Phylogenetic analysis of L protein sequences firmly establishes CoRSV as a member of the recently proposed Dichorhavirus genus. Predictive algorithms, in planta protein expression, and a yeast-based nuclear import assay were used to determine the nucleophillic character of five CoRSV proteins. Finally, the temperature-dependent ability of CoRSV to establish systemic infections in an initially local lesion host was quantified. - Highlights: • We report genome sequence determination for Coffee ringspot virus (CoRSV). • CoRSV should be considered a member of the proposed Dichorhavirus genus. • We report temperature-dependent systemic infection of an initially local lesion host. • We report in planta protein and localization data for five CoRSV proteins. • In silico predictions of the CoRSV proteins were validated using in vivo assays.

  10. Characterization of Coffee ringspot virus-Lavras: A model for an emerging threat to coffee production and quality

    International Nuclear Information System (INIS)

    Ramalho, T.O.; Figueira, A.R.; Sotero, A.J.; Wang, R.; Geraldino Duarte, P.S.; Farman, M.; Goodin, M.M.

    2014-01-01

    The emergence of viruses in Coffee (Coffea arabica and Coffea canephora), the most widely traded agricultural commodity in the world, is of critical concern. The RNA1 (6552 nt) of Coffee ringspot virus is organized into five open reading frames (ORFs) capable of encoding the viral nucleocapsid (ORF1p), phosphoprotein (ORF2p), putative cell-to-cell movement protein (ORF3p), matrix protein (ORF4p) and glycoprotein (ORF5p). Each ORF is separated by a conserved intergenic junction. RNA2 (5945 nt), which completes the bipartite genome, encodes a single protein (ORF6p) with homology to RNA-dependent RNA polymerases. Phylogenetic analysis of L protein sequences firmly establishes CoRSV as a member of the recently proposed Dichorhavirus genus. Predictive algorithms, in planta protein expression, and a yeast-based nuclear import assay were used to determine the nucleophillic character of five CoRSV proteins. Finally, the temperature-dependent ability of CoRSV to establish systemic infections in an initially local lesion host was quantified. - Highlights: • We report genome sequence determination for Coffee ringspot virus (CoRSV). • CoRSV should be considered a member of the proposed Dichorhavirus genus. • We report temperature-dependent systemic infection of an initially local lesion host. • We report in planta protein and localization data for five CoRSV proteins. • In silico predictions of the CoRSV proteins were validated using in vivo assays

  11. Viability of drying coffee using liquefied petroleum gas-LPG, firewood eucalyptus and husks of coffee; Viabilidade da secagem do cafe usando GLP, lenha de eucalipto e palha de cafe

    Energy Technology Data Exchange (ETDEWEB)

    Sobrinho, J.C.; Silva, J.N. da; Silva, J.S.; Lacerda Filho, A.F. de [Vicosa Univ., MG (Brazil). Dept. de Engenharia Agricola]. E-mail: jcardoso@alunos.ufv.br; jadir@mail.ufv.br; desousae@mail.ufv.br; alacerda@mail.ufv.br

    2000-07-01

    An analysis of the specific consumption of energy and of the comparative costs of the use of the Liquefied Petroleum Gas (LPG), eucalyptus firewood and coffee husks as sources of energy in the drying of coffee (Coffea arabica L.) was done. Dryers of intermittent crossed flows were used with temperature of the drying air of 60 deg C. The drying in yard was used as witness. It is ended that the use in the different form of energy did not alter the final quality of the product, and that LPG is more efficient in terms of specific consumption of energy that the husks of coffee and eucalyptus firewood, even so, of cost approximately four times or more higher than that of the other fuels. (author)

  12. New Coffee Plant-Infecting Xylella fastidiosa Variants Derived via Homologous Recombination.

    Science.gov (United States)

    Jacques, Marie-Agnès; Denancé, Nicolas; Legendre, Bruno; Morel, Emmanuelle; Briand, Martial; Mississipi, Stelly; Durand, Karine; Olivier, Valérie; Portier, Perrine; Poliakoff, Françoise; Crouzillat, Dominique

    2015-12-28

    Xylella fastidiosa is a xylem-limited phytopathogenic bacterium endemic to the Americas that has recently emerged in Asia and Europe. Although this bacterium is classified as a quarantine organism in the European Union, importation of plant material from contaminated areas and latent infection in asymptomatic plants have engendered its inevitable introduction. In 2012, four coffee plants (Coffea arabica and Coffea canephora) with leaf scorch symptoms growing in a confined greenhouse were detected and intercepted in France. After identification of the causal agent, this outbreak was eradicated. Three X. fastidiosa strains were isolated from these plants, confirming a preliminary identification based on immunology. The strains were characterized by multiplex PCR and by multilocus sequence analysis/typing (MLSA-MLST) based on seven housekeeping genes. One strain, CFBP 8073, isolated from C. canephora imported from Mexico, was assigned to X. fastidiosa subsp. fastidiosa/X. fastidiosa subsp. sandyi. This strain harbors a novel sequence type (ST) with novel alleles at two loci. The two other strains, CFBP 8072 and CFBP 8074, isolated from Coffea arabica imported from Ecuador, were allocated to X. fastidiosa subsp. pauca. These two strains shared a novel ST with novel alleles at two loci. These MLST profiles showed evidence of recombination events. We provide genome sequences for CFBP 8072 and CFBP 8073 strains. Comparative genomic analyses of these two genome sequences with publicly available X. fastidiosa genomes, including the Italian strain CoDiRO, confirmed these phylogenetic positions and provided candidate alleles for coffee plant adaptation. This study demonstrates the global diversity of X. fastidiosa and highlights the diversity of strains isolated from coffee plants. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  13. TRADITIONAL USE OF THREE EDIBLE INSECTS IN COFFEE AGROECOSYSTEMS IN THE STATE OF VERACRUZ

    Directory of Open Access Journals (Sweden)

    Esteban Escamilla Prado

    2012-11-01

    Full Text Available Coffee (Coffea arabica L. is one of the most economically, socioculturally and environmentally important crops in Veracruz. Shade-grown coffee plantations provide environmental services and play a key role in biodiversity conservation. In coffee farms in Veracruz insects are an abundant natural resource, and part of the use of this resource is the consumption of some edible insects in certain coffee regions. The objective of this study was to know the traditional use of three species of edible insects in the coffee agroecosystem of Veracruz. During the period 2007-2012, an ethnoentomological study was conducted in coffee regions from central Veracruz. The insect species identified were the ants chicatanas (Atta mexicana Smith and Atta cephalotes Latreille in the municipality of Huatusco, the larva gusano del jonote (Arsenura armida armida Cramer in the municipalities of Zongolica, Tequila and Tezonapa, and the larva gusanillo (Phassus triangularis H.E. in the municipalities of Córdoba, Ixhuatlán del Café, Tepatlaxco, Chocamán and Zongolica. The results showed the traditional knowledge held by coffee growers related to these edible species which are a valuable natural resource in their coffee plantations. Knowledge on agroecological relationships, collection, consumption and marketing was rescued. In conclusion, the insect species studied are used for local consumption and have great economic potential due to their high sell price during the harvest season.

  14. Características fisiológicas e de crescimento de cafeeiro sombreado com guandu e cultivado a pleno sol Physiological characteristics and growth of coffee plants grown under shade of pigeonpea and unshaded

    Directory of Open Access Journals (Sweden)

    Heverly Morais

    2003-10-01

    Full Text Available O conhecimento dos efeitos do sombreamento sobre a fisiologia de cafeeiros é importante para se determinar níveis ótimos de radiação e temperatura, bem como para subsidiar estudos sobre o crescimento de plantas sombreadas, a fim de determinar a arquitetura ideal do cafeeiro que maximize a captura da radiação solar disponível em ambientes sombreados. O objetivo deste trabalho foi avaliar características fisiológicas e de crescimento de cafeeiros (Coffea arabica L. cultivados sob sombreamento denso com guandu (Cajanus cajan (L. Millsp. e a pleno sol. O baixo nível de radiação incidente sobre os cafeeiros sombreados com guandu resultou em decréscimos na taxa fotossintética e na transpiração, maior altura de planta, folhas maiores e com menor quantidade de matéria seca. Esses resultados indicam que o excesso de sombra afeta drasticamente a fisiologia e morfologia de C. arabica.The characterization of shade effects on the physiology of coffee is important to determine optimum levels of radiation and temperature, as well as to subsidize studies on growth of shaded plants aiming at determining the ideal coffee plant architecture that maximizes the capture of the available solar radiation in shaded environments. The objective of this work was to evaluate physiological and growth characteristics of coffee (Coffea arabica L. shaded with pigeonpea (Cajanus cajan (L. Millsp. and under full sun. The low level of incident radiation on the coffee shaded with pigeonpea caused decreases in the photosynthetic rate and transpiration, increased plant height and leaf size, but diminished leaf dry matter. These results indicate that the excess of shade drastically affects the physiology and morphology of C. arabica.

  15. Simple procedure for nutrient analysis of coffee plant with energy dispersive X-ray fluorescence spectrometry (EDXRF)

    Energy Technology Data Exchange (ETDEWEB)

    Tezotto, Tiago; Favarin, Jose Laercio; Neto, Ana Paula; Azevedo, Ricardo Antunes, E-mail: tiago.tezotto@usp.br [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil); Gratao, Priscila Lupino [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP/ FCAV), Jaboticabal, SP (Brazil). Dept. de Biologia Aplicada a Agropecuaria; Mazzafera, Paulo [Universidade Estadual de Campinas (UNICAMP/IB), SP (Brazil). Dept. Biologia Vegetal

    2013-07-15

    Nutrient analysis is used to estimate nutrient content of crop plants to manage fertilizer application for sustained crop production. Direct solid analysis of agricultural and environmental samples by energy dispersive X-ray fluorescence spectrometry (EDXRF) was chosen as alternative technique to evaluate the simultaneous multielemental quantification of the most important essential elements in coffee (Coffea arabica L.) plants. Inductively coupled plasma atomic emission spectrometry and certified reference materials made from leaves were used to calibrate and check the trueness of EDXRF method for the determination of the concentration of several nutrients in coffee leaves and branches. Fluorescence spectrometry proved to be advantageous and presented low cost as loose powder samples could be used. Samples collected from a field experiment where coffee plants were treated with excess of Ni and Zn were used to verify the practical application of the method. Good relationships were achieved between certified values and data obtained by EDXRF, with recoveries ranging from 82 to 117 %.(author)

  16. Trocas gasosas de cafeeiros (Coffea arabica L. e seringueiras (Hevea brasiliensis Muell. Arg. em diferentes sistemas de cultivo na região de larvas, MG Gas exchange in coffe (Coffea arabica L. and rubber tree (Hevea brasiliensis Muell. Arg. in different crop systems in lavras, MG

    Directory of Open Access Journals (Sweden)

    Cláudio Roberto Meira de Oliveira

    2006-04-01

    Full Text Available O uso de sistemas de cultivo agrícola que favorecem a conservação dos recursos naturais e a diversidade de produção representa uma alternativa para produtores que visam menores custos em uma produção sustentável. Dentre esses sistemas de cultivo, destaca-se o sistema de consórcio entre cafeeiros e seringueiras, que tem sido estudado na região sul de Minas Gerais. Nesse contexto, avaliaram-se características de trocas gasosas, fluorescência da clorofila e relações hídricas de cafeeiros e seringueiras, em monocultivo e consórcio, na fase de implantação dos cultivos, com o objetivo de verificar o comportamento dessas espécies em diferentes sistemas de cultivo. Os resultados apontaram que um ambiente caracterizado por níveis de radiação e temperatura menores favorece as trocas gasosas do cafeeiro, enquanto maior disponibilidade hídrica e valores elevados de radiação são favoráveis ao desenvolvimento da seringueira quando em sistemas consorciados.Crop systems that improve conservation of natural resources and diversity of production is a promising practice for farmers seeking low costs and a sustainable agriculture. The coffee - rubber tree intercropping has been studied in the south of Minas Gerais - Brazil. Within this context, the objective of this work was to evaluate the characteristics gas exchange, chlorophyll fluorescence and water relations of coffee and rubber tree in both intercropping and monocroping systems, in the early stage of the plantation establishment. The results showed that the reduction in radiation levels and temperature promote gas exchange increase in coffee, while higher photosynthetic radiation values and soil water availability are favorable to rubber tree in intercropping systems.

  17. Pengujian sifat unggul beberapa klon harapan kopi arabika di kebun percobaan Andungsari, Jawa Timur (Testing for superior traits of some arabica coffee promising clones at Andungsari research station, East Java

    Directory of Open Access Journals (Sweden)

    Retno Hulupi

    2012-08-01

    Full Text Available One of the major diseases which limiting production in arabica coffee is the leaf rust caused by the fungus Hemileia vastatrix, B et Br. Selection and testing on thirteen promising arabica coffee clones were carried out at endemic area for leaf rust disease, Andungsari Research Station, for six fruiting times. The aims of these test were to find out superior planting material as clone with genetic resistance to leaf rust. As the beverage commodities, criterium selections for superiority clone besides resistant to leaf rust and yielding ability of more than 1,5 ton/ha also must be excellent in cup quality. Under this consideration, BP 416 A clone showed as the best high yielding ability i.e. 1,595 kg/ha and stable, besides resistant to leaf rust disease compared to the other clones tested. This clone had good cup quality, better than earlier released variety such as USDA 762 and S 795 although was not better than Andungsari 1 that were planted in the same location. The best physical bean characteristics was obtained on BP 513 A clone wich is derived from S 795 x Caturra red, having 89.2% of normal bean and 18.3% outurn characteristics. However, due to lower yielding and more susceptible to leaf rust, this clone could not be classified as superior clone.

  18. Adaptive Measures for the Factors Affecting Marketing of Coffee ...

    African Journals Online (AJOL)

    Adaptive Measures for the Factors Affecting Marketing of Coffee ( Coffea robusta ... of coffee in the study area was poor pricing and marketing systems; this is as a ... of quality control and relevant information on improved coffee technologies.

  19. Pest Management Strategies Against the Coffee Berry Borer (Coleoptera: Curculionidae: Scolytinae).

    Science.gov (United States)

    Infante, Francisco

    2018-03-22

    Coffee ( Coffea arabica and C. canephora) is one of the most widely traded agricultural commodities and the main cash crop in ∼80 tropical countries. Among the factors that limit coffee production, the coffee berry borer, Hypothenemus hampei (Ferrari) has been considered the main insect pest, causing losses of over U.S. $500 million dollars annually. Control of this pest has been hindered by two main factors: the cryptic nature of the insect (i.e., protected inside the coffee berry) and the availability of coffee berries in the field allowing the survival of the pest from one generation to the next. Coffee berry borer control has primarily been based on the use of synthetic insecticides. Management strategies have focused on the use of African parasitoids ( Cephalonomia stephanoderis, Prorops nasuta, and Phymastichus coffea), fungal entomopathogens ( Beauveria bassiana), and insect traps. These approaches have had mixed results. Recent work on the basic biology of the insect has provided novel insights that might be useful in developing novel pest management strategies. For example, the discovery of symbiotic bacteria responsible for caffeine breakdown as part of the coffee berry borer microbiome opens new possibilities for pest management via the disruption of these bacteria. Some chemicals with repellent propieties have been identified, and these have a high potential for field implementation. Finally, the publication of the CBB genome has provided insights on the biology of the insect that will help us to understand why it has been so successful at exploiting the coffee plant. Here I discuss the tools we now have against the CBB and likely control strategies that may be useful in the near future.

  20. Efeitos fisiológicos da coffea arábica e coffea canephora

    OpenAIRE

    Rodrigues, Tânia Patrícia Barradas

    2014-01-01

    Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz O café é uma das bebidas mais consumidas no mundo, sendo ultrapassado apenas pela água. De entre as várias espécies de café identificadas, Coffea arabica L. (arabica) e Coffea canephora Pierre (robusta), representam a grande maioria do café consumido no mundo. Para além da espécie de café, o tipo de processamento a que os grãos são submetidos (via seca ou húmida), o grau de torra e o método...

  1. Source-sink manipulations in Coffea arabica L. and its effect on growth of shoots and root system Manipulação da relação fonte-dreno em Coffea arabica L. e seu efeito no crescimento da parte aérea e do sistema radicular

    Directory of Open Access Journals (Sweden)

    José Donizeti Alves

    2011-10-01

    Full Text Available This paper investigated changes in growth and carbohydrate content of shoots and root systems in response to source-sink manipulation in field-grown coffee trees. The source-sink manipulations were carried out using manual de-fruiting. Two plots of trees were established: in one group, the fruit was left on the tree, while in the second group all fruits were removed manually. Similar results were obtained for coffee trees with and without fruit in terms of height, stem and skirt diameter and branch lengths. A biochemical evaluation of the roots showed that no differences were found between the levels of carbohydrates. In the leaves, levels of soluble sugars and reducing sugars were higher in plants with fruit. The starch concentration in leaves and roots did not vary between the plots. Root length density did not vary between plants with and without fruit in the 0 - 0.4 m soil layer. However, plants without fruit had a higher root length density than plants with a full fruit load. These results contrast with results published by others on source-sink relationships of coffee plants. The joint data analysis shows that young trees, with a moderate fruit load, and with good nutritional and health status, produce carbohydrates in sufficient quantities to maintain vegetative and reproductive growth, without harming root system growth.Foram investigadas as alterações no crescimento e nos teores de carboidratos da parte aérea e sistema radicular em resposta à manipulação fonte-dreno em cafeeiros cultivados em condições de campo. Essas manipulações foram realizadas mediante desfrutificação manual. Dois grupos de plantas foram estabelecidos: em um grupo, a produção de frutos foi mantida, enquanto no segundo grupo, todos os frutos foram retirados manualmente. Cafeeiros, com e sem frutos apresentaram resultados semelhantes em termos de altura, diâmetro do caule e saia e comprimentos de ramos. Nas avaliações bioquímicas nas raízes n

  2. Detection of enterotoxins produced by B. cereus through PCR analysis of ground and roasted coffee samples in Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Cyllene de Matos Ornelas da Cunha Corrêa de Souza

    2011-06-01

    Full Text Available Coffee is one of the most appreciated drinks in the world. Coffee ground is obtained from the fruit of a small plant that belongs to the genus Coffea. Coffea arabica and Coffea canephora robusta are the two most commercially important species. They are more commonly known as arabica and robusta, respectively. Two-thirds of Coffea arabica plants are grown in South and Central America, and Eastern Africa - the place of origin for this coffee species. Contamination by microorganisms has been a major matter affecting coffee quality in Brazil, mainly due to the harvesting method adopted. Brazilian harvests are based on fruits collected from the ground mixed with those that fall on collection cloths. As the Bacillus cereus bacterium frequently uses the soil as its environmental reservoir, it is easily capable of becoming a contaminant. This study aimed to evaluate the contamination and potential of B. cereus enterotoxin genes encoding the HBL and NHE complexes, which were observed in strains of ground and roasted coffee samples sold in Rio de Janeiro. The PCR (Polymerase Chain Reaction results revealed high potential of enterotoxin production in the samples. The method described by Speck (1984 was used for the isolation of contaminants. The investigation of the potential production of enterotoxins through isolates of the microorganism was performed using the B. cereus enterotoxin Reverse Passive Latex Agglutination test-kit (BCET-RPLA, Oxoid, according to the manufacturer's instructions. The potential of enterotoxin production was investigated using polymerase chain reaction (PCR methods for hblA, hblD and hblC genes (encoding hemolysin HBL and for nheA, nheB and nheC genes (encoding non-hemolytic enterotoxin - NHE. Of all the 17 strains, 100% were positive for at least 1 enterotoxin gene; 52.9% (9/17 were positive for the 3 genes encoding the HBL complex; 35.3% (6/17 were positive for the three NHE encoding genes; and 29.4% (5/17 were positive for

  3. Discrimination of organic coffee via Fourier transform infrared-photoacoustic spectroscopy.

    Science.gov (United States)

    Gordillo-Delgado, Fernando; Marín, Ernesto; Cortés-Hernández, Diego Mauricio; Mejía-Morales, Claudia; García-Salcedo, Angela Janet

    2012-08-30

    Procedures for the evaluation of the origin and quality of ground and roasted coffee are constantly needed for the associated industry due to complexity of the related market. Conventional Fourier transform infrared (FTIR) spectroscopy can be used for detecting changes in functional groups of compounds, such as coffee. However, dispersion, reflection and non-homogeneity of the sample matrix can cause problems resulting in low spectral quality. On the other hand, sample preparation frequently takes place in a destructive way. To overcome these difficulties, in this work a photoacoustic cell has been adapted as a detector in a FTIR spectrophotometer to perform a study of roasted and ground coffee from three varieties of Coffea arabica grown by organic and conventional methods. Comparison between spectra of coffee recorded by FTIR-photoacoustic spectrometry (PAS) and by FTIR spectrophotometry showed a better resolution of the former method, which, aided by principal components analysis, allowed the identification of some absorption bands that allow the discrimination between organic and conventional coffee. The results obtained provide information about the spectral behavior of coffee powder which can be useful for establishing discrimination criteria. It has been demonstrated that FTIR-PAS can be a useful experimental tool for the characterization of coffee. Copyright © 2012 Society of Chemical Industry.

  4. Effects of coffee management on deforestation rates and forest integrity.

    Science.gov (United States)

    Hylander, Kristoffer; Nemomissa, Sileshi; Delrue, Josefien; Enkosa, Woldeyohannes

    2013-10-01

    Knowledge about how forest margins are utilized can be crucial for a general understanding of changes in forest cover, forest structure, and biodiversity across landscapes. We studied forest-agriculture transitions in southwestern Ethiopia and hypothesized that the presence of coffee (Coffea arabica)decreases deforestation rates because of coffee's importance to local economies and its widespread occurrence in forests and forest margins. Using satellite images and elevation data, we compared changes in forest cover over 37 years (1973-2010) across elevations in 2 forest-agriculture mosaic landscapes (1100 km(2) around Bonga and 3000 km(2) in Goma-Gera). In the field in the Bonga area, we determined coffee cover and forest structure in 40 forest margins that differed in time since deforestation. Both the absolute and relative deforestation rates were lower at coffee-growing elevations compared with at higher elevations (-10/20% vs. -40/50% comparing relative rates at 1800 m asl and 2300-2500 m asl, respectively). Within the coffee-growing elevation, the proportion of sites with high coffee cover (>20%) was significantly higher in stable margins (42% of sites that had been in the same location for the entire period) than in recently changed margins (0% of sites where expansion of annual crops had changed the margin). Disturbance level and forest structure did not differ between sites with 30% or 3% coffee. However, a growing body of literature on gradients of coffee management in Ethiopia reports coffee's negative effects on abundances of forest-specialist species. Even if the presence of coffee slows down the conversion of forest to annual-crop agriculture, there is a risk that an intensification of coffee management will still threaten forest biodiversity, including the genetic diversity of wild coffee. Conservation policy for Ethiopian forests thus needs to develop strategies that acknowledge that forests without coffee production may have higher deforestation

  5. A High-Throughput Molecular Pipeline Reveals the Diversity in Prevalence and Abundance of Pratylenchus and Meloidogyne Species in Coffee Plantations.

    Science.gov (United States)

    Bell, Christopher A; Atkinson, Howard J; Andrade, Alan C; Nguyen, Hoa X; Swibawa, I Gede; Lilley, Catherine J; McCarthy, James; Urwin, P E

    2018-05-01

    Coffee yields are adversely affected by plant-parasitic nematodes and the pathogens are largely underreported because a simple and reliable identification method is not available. We describe a polymerase chain reaction-based approach to rapidly detect and quantify the major Pratylenchus and Meloidogyne nematode species that are capable of parasitizing coffee. The procedure was applied to soil samples obtained from a number of coffee farms in Brazil, Vietnam, and Indonesia to assess the prevalence of these species associated both with coffee (Coffea arabica and C. canephora) and its intercropped species Musa acuminata (banana) and Piper nigrum (black pepper). Pratylenchus coffeae and P. brachyurus were associated with coffee in all three countries but there were distinct profiles of Meloidogyne spp. Meloidogyne incognita, M. exigua, and M. paranaensis were identified in samples from Brazil and M. incognita and M. hapla were detected around the roots of coffee in Vietnam. No Meloidogyne spp. were detected in samples from Indonesia. There was a high abundance of Meloidogyne spp. in soil samples in which Pratylenchus spp. were low or not detected, suggesting that the success of one genus may deter another. Meloidogyne spp. in Vietnam and Pratylenchus spp. in Indonesia were more numerous around intercropped plants than in association with coffee. The data suggest a widespread but differential nematode problem associated with coffee production across the regions studied. The issue is compounded by the current choice of intercrops that support large nematode populations. Wider application of the approach would elucidate the true global scale of the nematode problem and the cost to coffee production. [Formula: see text] Copyright © 2018 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

  6. Sourgrass densities affecting the initial growth and macronutrient content of coffee plants Densidades de capim-amargoso afetando o crescimento inicial e o teor de macronutrientes do cafeeiro

    Directory of Open Access Journals (Sweden)

    L.B Carvalho

    2013-03-01

    Full Text Available The objective of this work was to evaluate the coexistence effects of coffee (Coffea arabica with densities of sourgrass (Digitaria insularis on crop macronutrient content and plant growth. The experiment was conducted in plots where one coffee plant was maintained in coexistence with 0 (weed-free check, 1, 2, 4, 8, and 16 sourgrass plants, using a completely randomized design with three replicates. Reduction of coffee growth and macronutrient content, except P that increased, started when the coexistence occurred with sourgrass plants in a density of 1 plant per plot. In general, macronutrient content was reduced by 18-50%, while growth characteristics were reduced by 9-41%, when coffee plants coexisted with 16 plants of sourgrass. Thus, sourgrass competition for nutrients was a strong factor limiting coffee plant growth.O objetivo deste trabalho foi avaliar os efeitos da convivência do cafeeiro (Coffea arabica com densidades de capim-amargoso (Digitaria insularis sobre o teor de macronutrientes e o crescimento das plantas da cultura. O experimento foi conduzido em caixas enterradas no solo, onde uma planta de café foi mantida em convivência com 0 (testemunha livre de plantas daninhas, 1, 2, 4, 8 e 16 plantas de capim-amargoso, usando o delineamento inteiramente casualizado com três repetições. A redução no crescimento e no teor de macronutrientes do cafeeiro - exceto P, que aumentou - iniciou-se quando a convivência ocorreu com plantas de capim-amargoso na densidade de uma planta por caixa. Em geral, o teor de macronutrientes foi reduzido em 10-50%, enquanto as características de crescimento foram reduzidas em 9-41%, quando o cafeeiro conviveu com 16 plantas de capim-amargoso. Assim, a competição de capim-amargoso por nutrientes foi um forte fator limitante para o crescimento das plantas de café.

  7. PENGARUH PEMBERIAN LIMBAH KULIT KOPI (Coffea robusta L. TERHADAP PERTUMBUHAN CABAI KERITING (Capsicum annum L.

    Directory of Open Access Journals (Sweden)

    Zainal Berlian

    2015-08-01

    Full Text Available Curly chili (Capsicum annum L. is a kind of vegetable commodities which very demand among the Indonesian because of its spicy taste that can be used as a flavoring dishes and has a high economic value. The goal of this study is to determine the effect of bark compost coffee (Coffea robusta in growing media on the growth and development of plants curly chili (Capsicum annum L. and severe skin compost coffee (Coffea robusta which gives the maximum growth of the plant curly chili (Capsicum annum L.. This research is conducted in the Laboratory Science UIN Raden Fatah Palembang by using experimental methods and completely randomized design (CRD consisting of 4 treatments and 6 replications treatment namely: P0 = Without the addition of compost the coffee (Coffea robusta (control, P1= Adding compost the coffee (Coffea robusta 30 grams, P2= Adding compost the coffee (Coffea robusta 60 grams , P3 = Addition of compost skin (Coffea robusta coffee 90 grams. Data are analyzed by F test followed by a test BJND (Difference Distance Real Duncan. The parameters of this study are plant height, number of leaves (pieces, the amount of fruit, and fruit weight. The results show that the addition of compost the coffee (Coffea robusta 90 grams (treatment P3 gives a very real effect on the growth of plant height, number of leaves, number of fruits, and also fruit weight. The conclusion is compost the coffee (Coffea robusta gives effect to the addition of compost and bark coffee (Coffea robusta 90 grams provides maximum growth and development of the plant curly chili (Capsicum annum L..

  8. Seed-Specific Stable Expression of the α-AI1 Inhibitor in Coffee Grains and the In Vivo Implications for the Development of the Coffee Berry Borer.

    Science.gov (United States)

    Albuquerque, Érika V S; Bezerra, Caroline A; Romero, Juan V; Valencia, Jorge W A; Valencia-Jiménez, Arnubio; Pimenta, Lucas M; Barbosa, Aulus E A D; Silva, Maria C M; Meneguim, Ana M; Sá, Maria Eugênia L; Engler, Gilbert; de Almeida-Engler, Janice; Fernandez, Diana; Grossi-de-Sá, Maria F

    Genetic transformation of coffee ( Coffea spp.), the second most traded commodity worldwide, is an alternative approach to introducing features that cannot be introgressed by traditional crossings. The transgenic stability, heritability and quantitative and spatial expression patterns of the seed-specific promoter phytohemagglutinin (PHA-L) from Phaseolus vulgaris were characterized in genetically modified C. arabica expressing the α-amylase inhibitor-1 ( α-AI1 ) gene. The α-AI1 inhibitor shows considerable activity toward digestive enzymes of the coffee berry borer (CBB) Hypothenemus hampei . This insect pest expends its life cycle almost entirely in coffee berries. Transgene containment in the fruit is important to meeting food and environmental safety requirements for releasing genetically modified (GM) crops. PCR analysis of T2 coffee plants showed a Mendelian single-copy segregation pattern. Ectopic transgene expression was only detected in coffee grains, as demonstrated by reverse transcription-PCR analysis of different plant tissues. An intense immunocytochemical signal associated with α-AI1 protein expression was localized to endospermic cells. In addition, a delay in the larval development of CBB was observed after challenging transgenic coffee seeds with the insect. These results indicate that the PHA-L promoter might be a useful tool in coffee for the seed-specific expression of genes related to coffee bean productivity, quality and pest protection. The biotechnological applicability of the α-AI1 gene for controlling CBB is also discussed. This work is the first report showing a seed-specific transgene expression in coffee plants.

  9. The 'PUCE CAFE' Project: the first 15K coffee microarray, a new tool for discovering candidate genes correlated to agronomic and quality traits.

    Science.gov (United States)

    Privat, Isabelle; Bardil, Amélie; Gomez, Aureliano Bombarely; Severac, Dany; Dantec, Christelle; Fuentes, Ivanna; Mueller, Lukas; Joët, Thierry; Pot, David; Foucrier, Séverine; Dussert, Stéphane; Leroy, Thierry; Journot, Laurent; de Kochko, Alexandre; Campa, Claudine; Combes, Marie-Christine; Lashermes, Philippe; Bertrand, Benoit

    2011-01-05

    Understanding the genetic elements that contribute to key aspects of coffee biology will have an impact on future agronomical improvements for this economically important tree. During the past years, EST collections were generated in Coffee, opening the possibility to create new tools for functional genomics. The "PUCE CAFE" Project, organized by the scientific consortium NESTLE/IRD/CIRAD, has developed an oligo-based microarray using 15,721 unigenes derived from published coffee EST sequences mostly obtained from different stages of fruit development and leaves in Coffea Canephora (Robusta). Hybridizations for two independent experiments served to compare global gene expression profiles in three types of tissue matter (mature beans, leaves and flowers) in C. canephora as well as in the leaves of three different coffee species (C. canephora, C. eugenoides and C. arabica). Microarray construction, statistical analyses and validation by Q-PCR analysis are presented in this study. We have generated the first 15 K coffee array during this PUCE CAFE project, granted by Génoplante (the French consortium for plant genomics). This new tool will help study functional genomics in a wide range of experiments on various plant tissues, such as analyzing bean maturation or resistance to pathogens or drought. Furthermore, the use of this array has proven to be valid in different coffee species (diploid or tetraploid), drastically enlarging its impact for high-throughput gene expression in the community of coffee research.

  10. The 'PUCE CAFE' Project: the First 15K Coffee Microarray, a New Tool for Discovering Candidate Genes correlated to Agronomic and Quality Traits

    Directory of Open Access Journals (Sweden)

    Leroy Thierry

    2011-01-01

    Full Text Available Abstract Background Understanding the genetic elements that contribute to key aspects of coffee biology will have an impact on future agronomical improvements for this economically important tree. During the past years, EST collections were generated in Coffee, opening the possibility to create new tools for functional genomics. Results The "PUCE CAFE" Project, organized by the scientific consortium NESTLE/IRD/CIRAD, has developed an oligo-based microarray using 15,721 unigenes derived from published coffee EST sequences mostly obtained from different stages of fruit development and leaves in Coffea Canephora (Robusta. Hybridizations for two independent experiments served to compare global gene expression profiles in three types of tissue matter (mature beans, leaves and flowers in C. canephora as well as in the leaves of three different coffee species (C. canephora, C. eugenoides and C. arabica. Microarray construction, statistical analyses and validation by Q-PCR analysis are presented in this study. Conclusion We have generated the first 15 K coffee array during this PUCE CAFE project, granted by Génoplante (the French consortium for plant genomics. This new tool will help study functional genomics in a wide range of experiments on various plant tissues, such as analyzing bean maturation or resistance to pathogens or drought. Furthermore, the use of this array has proven to be valid in different coffee species (diploid or tetraploid, drastically enlarging its impact for high-throughput gene expression in the community of coffee research.

  11. Determination of the Element Contents in Turkish Coffee and Effect of Sugar Addition

    OpenAIRE

    M. M. Fercan; A. S. Kipcak; O. Dere Ozdemir; M. B. Piskin; E. Moroydor Derun

    2016-01-01

    Coffee is a widely consumed beverage with many components such as caffeine, flavonoids, phenolic compounds, and minerals. Coffee consumption continues to increase due to its physiological effects, its pleasant taste, and aroma. Robusta and Arabica are two basic types of coffee beans. The coffee bean used for Turkish coffee is Arabica. There are many elements in the structure of coffee and have various effect on human health such as Sodium (Na), Boron (B), Magnesium (Mg) and Iron (Fe). In this...

  12. Quality of wholemeal wheat bread enriched with green coffee beans

    Directory of Open Access Journals (Sweden)

    Urszula Gawlik-Dziki

    2016-01-01

    Full Text Available Scientific studies have revealed that bioactive components of coffee play a preventive role against various degenerative diseases. Green coffee, in particular, is characterized by its unique composition and properties. The objective of this work was to investigate the influence of green coffee (Coffea arabica beans (GCB addition on the quality and antioxidant properties (AA of the wholemeal bread. For bread preparation, flour form GCB, and wholemeal wheat flour, type 2000 were used. Wholemeal wheat flour was replaced with GCB flour at 1 to 5% levels. Loaf volume, texture, color and sensory properties of bread were determined. Furthermore, total phenolic content and antioxidant activity were evaluated. The results showed that bread supplementation with GCB had little influence on the bread volume. The highest volume of bread was obtained with 3 and 4% of GCB flour. The texture properties of bread crumb (hardness, elasticity, cohesiveness and chewiness were slightly changed as a result of the GCB addition. The lightness of bread crumb decreased with the GCB addition (average from 46.3 to 42.6. Besides, the addition of GCB significantly enriched wheat bread with hydrophilic phenolic compounds. The phenolic compounds were highly bioaccessible in vitro. Moreover, the GCB addition enhanced antiradical activity of bread.

  13. Cercosporiose progression in the agroforestry consortium coffee-rubber trees

    Directory of Open Access Journals (Sweden)

    Humberto Godoy Androcioli

    2015-12-01

    Full Text Available Cercospora coffeicola is one of the primary diseases that affect coffee plants. Studies indicate that shaded coffee plants reduce the incidence of this disease and that the management of trees and coffee plants arrangement influence in the dissemination of cercospora. The objective of this study was to evaluate the incidence and severity of C. coffeicola at different distances from double rows of rubber trees (Hevea brasiliensis muell. arg. at two different sunlight exposures (north and south. This study was conducted in Londrina, Parana, between 2008 and 2010, with adult plants of the IAPAR 59 cultivar (Coffea arabica L. grown at a spacing of 2.5 m x 1.0 m. The distances between the double rows of rubber trees were 13, 16 and 22 m, compared to plants grown under full sun. The disease incidence was assessed monthly by using a non-destructive method. This analysis was conducted on coffee leaves from the third and fourth pairs of two plagiotropic branches, on eight plants per plot, with five replications. These data were used to calculate the area under the curve for the incidence of the brown eye spot. The highest disease incidence occurred in the coffee plants grown under full sun, whereas lowest disease occurred on plants located at up to two meters away from double rows of rubber trees. The incidence of Cercospora leaf spot increased with the distance from the double rows of rubber trees. The results demonstrate that the mapping of cercospora incidence in shaded coffee plants is essential to determinate the best spacing and plants arrangement.

  14. Partial resistance to fruit necrosis associated to Colletotrichum spp. among arabic coffee genotypes

    Directory of Open Access Journals (Sweden)

    Gustavo Hiroshi Sera

    2007-05-01

    Full Text Available The aim of this study was to evaluate the resistance to fruit necrosis associated to Colletotrichum spp. in Coffea arabica L.. Twenty-two arabic coffee genotypes were performed in a randomized block design, with three replications and ten plants per plot. They were evaluated for the variables fruit necrosis, luminosity index inside of the plant canopy and fruits per productive nodes of the plants. There was genetic variability for the resistance to fruit necrosis. Different partial resistance levels, varying from highly susceptible (score = 3.89 to moderately resistant (score = 1.77 were observed. The genotypes that presented moderate resistance were the cultivars IPR-100, IPR-103, IPR-105 and IPR-108 and the coffee selection IAPAR-00023.Diferentes espécies de fungo do gênero têm sido associadas a perdas na cafeicultura brasileira. O objetivo deste trabalho foi avaliar a resistência à necrose de frutos associada ao Colletotrichum spp. em Coffea arabica L.. Vinte e dois genótipos de café foram avaliados no delineamento em blocos ao acaso com três repetições e dez plantas por parcela. As avaliações foram realizadas no IAPAR, Londrina, em janeiro de 2004 para as variáveis necrose de frutos, índice de luminosidade dentro da copa da planta e frutos por nó produtivo. Existe variabilidade genética em cafeeiros arábicos para a resistência à necrose de frutos. Foi observado diferentes níveis de resistência parcial, variando de altamente suscetível (nota = 3,89 a moderadamente resistente (nota = 1,77. Os genótipos que apresentaram moderada resistência foram as cultivares IPR-100, IPR-103, IPR-105 e IPR-108 e a seleção de café IAPAR-00023.

  15. Vegetative and productive aspects of organically grown coffee cultivars under shaded and unshaded systems

    Directory of Open Access Journals (Sweden)

    Marta dos Santos Freire Ricci

    2011-08-01

    Full Text Available Although Coffea arabica species has its origin in the African understories, there is great resistance on the part of the Brazilian producers for growing this species under agroforestry systems as they fear that shading reduces production. This study aimed at evaluating some vegetative traits and the productivity of organically grown coffee (Coffea arabica L. cultivars under shaded and unshaded systems. Twelve treatments consisting of two cultivation systems (shaded and unshaded and six coffee cultivars were arranged in randomized blocks with four replicates, in a split-plot scheme. Shading was provided by banana (Musa sp. and coral bean plants (Erythrinaverna. Shading delayed fruit maturation. Late maturation cultivars, such as the Icatu and the Obatã, matured early in both cultivation systems, while medium and early maturation cultivars presented late maturation. Cultivation in the shaded system increased the leaf area and the number of lower branches, decreased the number of productive nodes per branch, and increased the distance between the nodes and the number of leaves present in the branches. Cultivation in the unshaded system presented greater number of plants with branch blight in relation to plants grown in the shade. The productivity of the cultivars was not different, at 30.0 processed bags per hectare in the shaded system, and 25.8 processed bags per hectare in the unshaded system. The most productive cultivars in the shaded system were the Tupi, the Obatã, and the Catuaí, while no differences between cultivars were obtained in the unshaded system.

  16. Influence of Storage of Wet Arabica Parchment Prior to Wet Hulling on Moulds Development, OchratoxinA Contamination, and Cup Quality of Mandheling Coffee

    Directory of Open Access Journals (Sweden)

    Cahya Ismayadi

    2005-08-01

    Full Text Available Mandheling coffee has been a well known specialty coffees for decades and the demand for this coffee is currently increasing. This coffee is characterised by low acidity, heavy-complex body, spicy-little earthy and fruity flavor. Mandheling coffee is produced by smallholder farmers in the highland surrounding Lake TobaNorth Sumatra in an unique way i.e. following de-pulping and 1–2 days sundrying, wet parchment is stored for varying periods up to a few weeks, the parchments are then de-hulled when still wet (40–45% moisture content then the beans sundried. The handling procedure presumably contributes to the unique cup character of Mandheling coffee. On the other hand the storage of wet pachments may cause mould growth and mycotoxin contamination. This trial was designed to study the influence of storage of wet parchments prior to wet hulling on mould development, OTA contamination and cup Mandheling characteristic of the coffee product. The normal wet process, drying of parchment thoroughly to 12% moisture content was used as the control. Parchment coffees (6 lots used for this trial were drawn from farmers and collectors in the region. The wet parchments (41.74–53.96% moisture content were stored for 1 (D1, 7 (D7 and 14 (D14 days in PE sacks in a warehouse in the region. During the storage period, when there was visible mould growth, the parchments were spread on a plastic sheet inside the warehouse, as per common practice to suppress the mould growth. Following storage, the wet parchment was de-hulled and then sun-dried to a moisture content of 12% (MC12% or dried to a moisture content of 17%, and held in storage for 3 weeks prior to final drying to 12% mc. The ‘normal wet process’ i.e. fresh-non stored parchments dried thoroughly to 12%, were used as the control. Parameters measured were visual evaluation, mould infestation, a w, moisture content (MC on the stored parchment; while for dried beans mould infestation, OTA content and

  17. Coffee and spent coffee extracts protect against cell mutagens and inhibit growth of food-borne pathogen microorganisms

    OpenAIRE

    Cid, C. (Concepción); Peña, M.P. (María Paz) de; Arbillaga, L. (Leire); Vitas, A.I. (Ana Isabel); Bravo, J. (Jimena); Monente, C. (Carmen)

    2015-01-01

    Coffee consumption decreases the risk of oxidative stress-related diseases. The by-product obtained after brewing process (spent coffee) also has antioxidant capacity. Spent coffee and coffee brews (filter and espresso) extracts were obtained from Arabica and Robusta coffees, respectively. Spent coffee showed slightly high amounts in chlorogenic acids, but caffeine content was similar to their respective coffee brew. All samples exhibited strong protection activity against indirect acting mut...

  18. Effect of fungal infection on phenolic compounds during the storage of coffee beans

    Directory of Open Access Journals (Sweden)

    Amal, A. A.

    2013-12-01

    Full Text Available Aims: This work was undertaken to study the effect of Aspergillus infection on phenolic compounds in beans from four cultivars of the coffee plant (Coffea arabica L.. The effects of storage conditions of the coffee beans were also examined. Methodology and results: Beans from four varieties of coffee were artificially infected with three species of Aspergillus: A. niger, A. melleus and A. alliacus, and stored at 0, 8 and 25 ± 2 °C. After 3, 6 and 9 months, the contents of phenolic compounds in the beans were determined using high performance liquid chromatography (HPLC. Conclusion, significance and impact study: The results of this study showed that phenolic compounds were qualitatively and quantitatively higher in the inoculated beans as compared with the uninfected control beans, reflecting a possible induced defense mechanism in the infected beans. Increased storage periods resulted in higher levels of phenols, but the average total, bound and free phenols did not differ between the cultivars tested. Effective control of Apergillus infection in coffee beans can prevent such changes in phenolics that may affect their commercial value.

  19. Contribuição do porta-enxerto, no teor de cafeína em grãos de café Contribution of the root-stock on the caffeine content of the coffee grains

    Directory of Open Access Journals (Sweden)

    M. Melo

    1976-01-01

    Full Text Available A contribuição do porta-enxerto no teor de cafeína dos grãos de café foi investigada nos cultivares mundo novo e laurina de Coffea arabica e no robusta de C. canephora, os quais diferem marcadamente no teor desse alcalóide. Os resultados obtidos indicam que o porta-enxerto, independentemente de seu genótipo, não exerce efeito mensurável sobre o teor de cafeína nos grãos. Variações individuais foram verificadas no teor de cafeína no robusta, devido à sua variabilidade genética, indicando a possibilidade de seleção para essa característica dentro desse material. Do ponto de vista agrícola o robusta poderia ser usado como porta-enxerto para as seleções de C. arabica, sem qualquer efeito no produto final.The contribution of the root-stock on the caffeine content of the coffee grains was studied in the Mundo Novo and Laurina cultivais of Coffea arabica and in the Rubosta cultivar of C. canephora which markedly differ in the alkaloid content. The results indicate that the root-stock, independently of its genotype, does not influence the caffeine content of the grains. Individual variation in caffeine content was found in the Robusta due to its genetic variability, pointing out to the possibility of selection for this characteristics in this material. It was also concluded that Robusta cultivars can be used as root-stock for Arabica selections without affecting the final product.

  20. Effects of roasting temperatures and gamma irradiation on the content of chlorogenic acid, caffeic acid and soluble carbohydrates of coffee

    International Nuclear Information System (INIS)

    Deshpande, S.N.; Aguilar, A.A.

    1975-01-01

    Two varieties of Puerto Rican coffee, Coffea canephora L. var. Robusta, and Coffea arabica L. var. Borbon, were subjected to four different doses of radiation and roasted at two different temperatures. Aqueous extracts of the ground coffee beans were analyzed for chlorogenic acid and caffeic acid at 324 nm and 360 nm wavelength settings, respectively. Samples subjected to the roasting treatments in conjuction with irradiation treatments were treated with basic lead acetate prior to the colorimetric analyses in order to eliminate interfering substances. The total carbohydrate content was also determined by colorimetric techniques with anthrone reagent. The total nitrogen content of the pulverized samples were determined by the micro-Kjeldahl method. While roasting treatments caused a reduction in the concentrations of the chlorogenic acid, caffeic acid, and the carbohydrates, the radiation treatments increased the concentrations of soluble carbohydrates without affecting the concentrations of chlorogenic acid or caffeic acid. It therefore appears that radiation treatments seem to cause degradation of the acid-polysaccharide complexes liberating soluble sugars. There were no noticable changes in the total content of nitrogen caused by roasting or the radiation treatments as indicated by the statistical analysis employing the split plot design. (author)

  1. Innovative Strategies for Control of Coffee Insect Pests in Tanzania ...

    African Journals Online (AJOL)

    Coffee insect pests are one of the major factors which affect coffee production and quality. globally, coffee insect pests are estimated to cause losses of about 13%. However in Africa, yield losses can be much higher, particularly where Arabica and Robusta coffee are grown for a long time. In Tanzania the major insect pests ...

  2. Drip irrigation in coffee crop under different planting densities: Growth and yield in southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Gleice A. de Assis

    2014-11-01

    Full Text Available Irrigation associated to reduction on planting spaces between rows and between coffee plants has been a featured practice in coffee cultivation. The objective of the present study was to assess, over a period of five consecutive years, influence of different irrigation management regimes and planting densities on growth and bean yield of Coffea arabica L.. The treatments consisted of four irrigation regimes: climatologic water balance, irrigation when the soil water tension reached values close to 20 and 60 kPa; and a control that was not irrigated. The treatments were distributed randomly in five planting densities: 2,500, 3,333, 5,000, 10,000 and 20,000 plants ha-1. A split-plot in randomized block design was used with four replications. Irrigation promoted better growth of coffee plants and increased yield that varied in function of the plant density per area. For densities from 10,000 to 20,000 plants ha-1, regardless of the used irrigation management, mean yield increases were over 49.6% compared to the non-irrigated crop.

  3. Sustained photosynthetic performance of Coffea spp. under long-term enhanced [CO2].

    Directory of Open Access Journals (Sweden)

    José C Ramalho

    Full Text Available Coffee is one of the world's most traded agricultural products. Modeling studies have predicted that climate change will have a strong impact on the suitability of current cultivation areas, but these studies have not anticipated possible mitigating effects of the elevated atmospheric [CO2] because no information exists for the coffee plant. Potted plants from two genotypes of Coffea arabica and one of C. canephora were grown under controlled conditions of irradiance (800 μmol m(-2 s(-1, RH (75% and 380 or 700 μL CO2 L(-1 for 1 year, without water, nutrient or root development restrictions. In all genotypes, the high [CO2] treatment promoted opposite trends for stomatal density and size, which decreased and increased, respectively. Regardless of the genotype or the growth [CO2], the net rate of CO2 assimilation increased (34-49% when measured at 700 than at 380 μL CO2 L(-1. This result, together with the almost unchanged stomatal conductance, led to an instantaneous water use efficiency increase. The results also showed a reinforcement of photosynthetic (and respiratory components, namely thylakoid electron transport and the activities of RuBisCo, ribulose 5-phosphate kinase, malate dehydrogenase and pyruvate kinase, what may have contributed to the enhancements in the maximum rates of electron transport, carboxylation and photosynthetic capacity under elevated [CO2], although these responses were genotype dependent. The photosystem II efficiency, energy driven to photochemical events, non-structural carbohydrates, photosynthetic pigment and membrane permeability did not respond to [CO2] supply. Some alterations in total fatty acid content and the unsaturation level of the chloroplast membranes were noted but, apparently, did not affect photosynthetic functioning. Despite some differences among the genotypes, no clear species-dependent responses to elevated [CO2] were observed. Overall, as no apparent sign of photosynthetic down

  4. Sustained Photosynthetic Performance of Coffea spp. under Long-Term Enhanced [CO2

    Science.gov (United States)

    Ramalho, José C.; Rodrigues, Ana P.; Semedo, José N.; Pais, Isabel P.; Martins, Lima D.; Simões-Costa, Maria C.; Leitão, António E.; Fortunato, Ana S.; Batista-Santos, Paula; Palos, Isabel M.; Tomaz, Marcelo A.; Scotti-Campos, Paula; Lidon, Fernando C.; DaMatta, Fábio M.

    2013-01-01

    Coffee is one of the world’s most traded agricultural products. Modeling studies have predicted that climate change will have a strong impact on the suitability of current cultivation areas, but these studies have not anticipated possible mitigating effects of the elevated atmospheric [CO2] because no information exists for the coffee plant. Potted plants from two genotypes of Coffea arabica and one of C. canephora were grown under controlled conditions of irradiance (800 μmol m-2 s-1), RH (75%) and 380 or 700 μL CO2 L-1 for 1 year, without water, nutrient or root development restrictions. In all genotypes, the high [CO2] treatment promoted opposite trends for stomatal density and size, which decreased and increased, respectively. Regardless of the genotype or the growth [CO2], the net rate of CO2 assimilation increased (34-49%) when measured at 700 than at 380 μL CO2 L-1. This result, together with the almost unchanged stomatal conductance, led to an instantaneous water use efficiency increase. The results also showed a reinforcement of photosynthetic (and respiratory) components, namely thylakoid electron transport and the activities of RuBisCo, ribulose 5-phosphate kinase, malate dehydrogenase and pyruvate kinase, what may have contributed to the enhancements in the maximum rates of electron transport, carboxylation and photosynthetic capacity under elevated [CO2], although these responses were genotype dependent. The photosystem II efficiency, energy driven to photochemical events, non-structural carbohydrates, photosynthetic pigment and membrane permeability did not respond to [CO2] supply. Some alterations in total fatty acid content and the unsaturation level of the chloroplast membranes were noted but, apparently, did not affect photosynthetic functioning. Despite some differences among the genotypes, no clear species-dependent responses to elevated [CO2] were observed. Overall, as no apparent sign of photosynthetic down-regulation was found, our data

  5. Influência da aplicação de dois resíduos industriais nas propriedades químicas de dois solos cultivados com café arábica Influence of the application of two types of industrial waste on the chemical properties of soil planted with coffea Arabica

    Directory of Open Access Journals (Sweden)

    Natiélia Oliveira Nogueira

    2012-03-01

    Full Text Available A grande quantidade de resíduos industriais que constituiu atualmente sérios problemas ambientais tem alertado para a utilização adequada desses materiais que, além de reduzir os impactos ambientais, tem demonstrado potencial para utilização na agricultura. O objetivo deste estudo foi avaliar os efeitos químicos da aplicação de diferentes doses de escória de siderurgia e óxido de magnésio em dois solos sob o cultivo de café arábica. O estudo foi desenvolvido em casa de vegetação do Centro de Ciências Agrárias da Universidade Federal do Espírito Santo, em vasos de 10 dm³. O delineamento experimental foi instalado em blocos casualizados, com distribuição fatorial de 2 x 3 x 6, com três repetições, sendo os fatores: dois solos (Latossolo Vermelho-Amarelo distrófico de textura argilosa e Latossolo Vermelho-Amarelo distrófico de textura média; três insumos (calcário como controle e escória de siderurgia e óxido de magnésio como resíduos; seis doses dos materiais corretivos (0; 25; 50; 75; 100 e 125% da necessidade de calagem. Após 180 dias de cultivo, procedeu-se as análises de pH, H+ + Al3+, Al3+, Ca2+ e Mg2+. Os resultados mostraram que as aplicações de doses crescentes de escória e óxido de magnésio até o limite estudado neste trabalho favorecem um decréscimo da acidez do solo. O óxido de magnésio demonstrou maior potencial como corretivo de acidez de solo e como fonte de magnésio para o solo.The large amount of industrial waste which currently causes serious environmental problems has demonstrated the need for the appropriate use of those materials which not only reduce environmental impact but have shown potential for agricultural use. The aim of this study was to evaluate the chemical effects of different amounts of slag and magnesium oxide on two soils planted with coffea arabica. The study was carried out using pots of 10 dm³ in a greenhouse at the Center for Agrarian Sciences of the Federal

  6. Influence of coffee/water ratio on the final quality of espresso coffee

    OpenAIRE

    Andueza, S. (Susana); Vila, M.A. (María A.); Peña, M.P. (María Paz) de; Cid, C. (Concepción)

    2007-01-01

    Espresso coffee is a polyphasic beverage in which the physico-chemical and sensory characteristics obviously depend on both the selection of ground roasted coffee and the technical conditions of the percolation process. The aim of this work was to evaluate the influence of the coffee/water ratio on the physico-chemical and sensory quality of espresso coffee. Furthermore, the influence of botanical varieties (Arabica and Robusta) and the type of roast (conventional and torrefacto) on the selec...

  7. Transferência de fatores genéticos de resistência a Hemileia vastatrix para o cultivar mundo novo Transference of the genes SH2 and SH3 for resistance to Hemileia vastatrix to the mundo novo cultivar of C. arabica

    Directory of Open Access Journals (Sweden)

    A. Carvalho

    1977-01-01

    Full Text Available Cafeeiros portadores dos fatores genéticos SH2 ou SH2 e SH3, simultaneamente, que conferem resistência a várias raças de Hemileia vastatrix, foram cruzados com plantas selecionadas do cultivar mundo novo de Coffea arabica a fim de se obter, em F2, recombinações com resistência a esse patógeno e elevada produtividade. Analisaram-se 14 populações F2 segregando apenas para o fator SH2, oito para os fatores SH2 e HS3, e três populações que dão, em sua descendência, plantas do grupo A, resistentes a todas as raças do patógeno até agora conhecidas. De 22.356 cafeeiros originalmente plantados em ensaio, a duas mudas por cova, em parcelas casualizadas, fez-se uma primeira seleção deixando apenas um cafeeiro por cova, reduzindo-se para 11.178 as plantas em estudo. Com base no aspecto vegetativo, na produtividade, na ausência de defeitos nos frutos e na reação de resistência ao agente causal da ferrugem, realizaram-se sucessivas seleções escolhendo-se finalmente, apenas 100 cafeeiros do tipo mundo novo e resistentes a H. vastatrix para derivação das populações F2 e prosseguimento da seleção.Coffee trees homozygous for the alleles SH2 or SH2 and SH3 which confer resistance to several physiological races of Hemileia vastatrix, were crossed to selected plants of Mundo Novo cultivar of Coffea arabica and the F2 generations were studied aiming to develop new high yielding and resistant coffee recombinations. A complete randomized field trial was stablished including 14 F2 populations segregating for SH2, eight populations segregating for SH2 and SH3 genes, and three populations segregating for plants of the A group of reaction to the H. vastatrix attack. A total of 22,356 F2 plants were analysed. Based on the plant vigor, yield capacity, percentage of normal developed seeds and resistance reaction to H. vastatrix, three successive series of selection were undertaken leaving only 100 coffee trees for development of F3 populations

  8. Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities.

    Science.gov (United States)

    Campos, Nádia A; Panis, Bart; Carpentier, Sebastien C

    2017-01-01

    One of the most important crops cultivated around the world is coffee. There are two main cultivated species, Coffea arabica and C. canephora. Both species are difficult to improve through conventional breeding, taking at least 20 years to produce a new cultivar. Biotechnological tools such as genetic transformation, micropropagation and somatic embryogenesis (SE) have been extensively studied in order to provide practical results for coffee improvement. While genetic transformation got many attention in the past and is booming with the CRISPR technology, micropropagation and SE are still the major bottle neck and urgently need more attention. The methodologies to induce SE and the further development of the embryos are genotype-dependent, what leads to an almost empirical development of specific protocols for each cultivar or clone. This is a serious limitation and excludes a general comprehensive understanding of the process as a whole. The aim of this review is to provide an overview of which achievements and molecular insights have been gained in (coffee) somatic embryogenesis and encourage researchers to invest further in the in vitro technology and combine it with the latest omics techniques (genomics, transcriptomics, proteomics, metabolomics, and phenomics). We conclude that the evolution of biotechnology and the integration of omics technologies offer great opportunities to (i) optimize the production process of SE and the subsequent conversion into rooted plantlets and (ii) to screen for possible somaclonal variation. However, currently the usage of the latest biotechnology did not pass the stage beyond proof of potential and needs to further improve.

  9. Agroforestry systems, nutrients in litter and microbial activity in soils cultivated with coffee at high altitude

    Directory of Open Access Journals (Sweden)

    Krystal de Alcantara Notaro

    2014-04-01

    Full Text Available Agroforestry systems are an alternative option for sustainable production management. These systems contain trees that absorb nutrients from deeper layers of the soil and leaf litter that help improve the soil quality of the rough terrain in high altitude areas, which are areas extremely susceptible to environmental degradation. The aim of this study was to characterize the stock and nutrients in litter, soil activity and the population of microorganisms in coffee (Coffea arabica L. plantations under high altitude agroforestry systems in the semi-arid region of the state of Pernambuco, Brazil. Samples were collected from the surface litter together with soil samples taken at two depths (0-10 and 10-20 cm from areas each subject to one of the following four treatments: agroforestry system (AS, native forest (NF, biodynamic system (BS and coffee control (CT.The coffee plantation had been abandoned for nearly 15 years and, although there had been no management or harvesting, still contained productive coffee plants. The accumulation of litter and mean nutrient content of the litter, the soil nutrient content, microbial biomass carbon, total carbon, total nitrogen, C/N ratio, basal respiration, microbial quotient, metabolic quotient and microbial populations (total bacteria, fluorescent bacteria group, total fungi and Trichoderma spp. were all analyzed. The systems thatwere exposed to human intervention (A and BS differed in their chemical attributes and contained higher levels of nutrients when compared to NF and CT. BS for coffee production at high altitude can be used as a sustainable alternative in the high altitude zones of the semi-arid region in Brazil, which is an area that is highly susceptible to environmental degradation.

  10. Determinants of crop diversity and composition in Enset-coffee agroforestry homegardens of Southern Ethiopia

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    Tesfaye Abebe

    2013-08-01

    Full Text Available Households in much of the tropics depend for their livelihoods on the variety and continued production of food and other products that are provided by their own farms. In such systems, maintenance of agrobiodiversity and ensuring food security are important for the well being of the population. The enset-coffee agroforestry homegardens of Southern Ethiopia that are dominated by two native perennial crops, Coffee (Coffea arabica L. and Enset (Enset ventricosum Welw. Cheesman, are examples of such agricultural systems. This study was conducted in Sidama administrative zone of Southern Ethiopia to determine the factors that influence the diversity and composition of crops in the systems. Data were collected from 144 sample homegardens selected from four districts. Stepwise multiple regression analysis was used to relate indices of crop diversity and area share of major crops with the physical and socioeconomic factors. The study revealed that socioeconomic factors, mainly proximity to markets, affected negatively crop species richness. The production area of the main crops enset and coffee decreased with increasing proximity to market and road while that of maize and khat increased. At household level, farm size had a significant effect on area share of enset and coffee. As farm size increased the share of the cash crop, coffee increased but that of the staple, enset declined. Enset, which is the backbone of the system in terms of food security, is declining on small farms and the share of monoculture maize system is increasing. The trend towards declining agrobiodiversity, and reduction in the production area of the main perennial crops and their gradual replacement with monoculture fields could make the systems liable to instability and collapse. As these sites are high potential agricultural areas, intensification can be achieved by integrating high-value and more productive crops, such as fruits, spices and vegetables, while maintaining the

  11. Carboidratos na bebida do café preparado sob diferentes processos Coffee carbohydrates in different preparations of the beverage

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    Marina Câmara Mattos Martins

    2005-06-01

    Full Text Available O café é uma das bebidas mais consumidas no mundo e seus polissacarídeos têm sido investigados devido a sua grande importância comercial. Os principais polissacarídeos de café são os galactomananos e arabinogalactanos, considerados como fibras alimentares não digeridas no sistema digestivo humano. O presente trabalho teve por objetivo quantificar e determinar a composição de monossacarídeos e de polissacarídeos na bebida do café. As preparações foram realizadas em coador de papel tradicional, em cafeteira elétrica e em máquina expresso, utilizando café Arábica (Coffea arabica. Determinações quantitativas e qualitativas dos açúcares foram realizadas, respectivamente, por análises colorimétricas e cromatografia de troca aniônica (HPAEC. O rendimento de sólidos solúveis foi maior em café expresso, sendo os valores cerca de 3,6 vezes superiores àqueles de café de cafeteira elétrica com filtro de papel. Preparações de café expresso foram as que resultaram em maiores teores de açúcares totais não redutores por volume de bebida. Entretanto, os valores de açúcares totais foram significativamente maiores na preparação em cafeteira elétrica, quando expressos pelo total de sólidos solúveis extraídos, indicando que o tempo de extração é provavelmente mais eficiente do que a temperatura. A composição em monossacarídeos foi similar em todas as amostras e independente do modo de preparação do café. Todos apresentaram arabinose (14-15%, galactose (29-30%, glucose (6% e manose 48-49%. Não se sabe se os polissacarídeos de café exercem as mesmas funções que a goma guar como fibra, mas, se isto ocorrer, esta é possivelmente uma das mais importantes fibras alimentares que ingerimos, levando em consideração a popularidade do café.Coffee is highly consumed worldwide and therefore one of the best studied beverages regarding chemical composition. Most of the soluble solids in coffee are carbohydrates

  12. High coffee population density to improve fertility of an oxisol Densidade populacional de cafeeiros para melhorar a fertilidade de um latossolo

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    Marcos Antonio Pavan

    1999-03-01

    Full Text Available The objective of this work was to evaluate the effect of coffee (Coffea arabica L. population densities on the chemical and microbiological properties of an Oxisol. The work was carried out on soil samples of 0-20 cm depth originated from an experimental site which had been used for coffee tree spacing studies during 15 years, in Paraná State, Brazil. Eight coffee tree populations were evaluated: 7143, 3571, 2381, 1786, 1429, 1190, 1020, and 893 trees/ha. Increasing plant population increased soil pH, exchangeable Ca, Mg, K, extractable P, organic carbon, moisture content and coffee root colonization by vesicular arbuscular mycorrhizal fungi, and decreased exchangeable Al and microbial biomass. Such results were attributed to better erosion control, improved plant residue management and nutrient cycling, and decreased leaching losses. Increasing coffee tree population per unit of area has shown to be an important reclamation recuperation strategy for improving fertility of the acid soils in Paraná, Brazil.O objetivo deste trabalho foi verificar os efeitos de diferentes densidades de população de cafeeiros (Coffea arabica L. nas propriedades químicas, físicas e microbiológicas do solo. Amostras de um Latossolo foram coletadas na profundidade 0-20 cm em um experimento de campo onde estavam sendo avaliadas diferentes populações de cafeeiros. Os tratamentos foram 7.143, 3.571, 2.381, 1.786, 1.429, 1.190, 1.020 e 893 covas/ha. Os resultados demonstraram que o aumento da população de plantas por unidade de área aumentou o pH, o Ca, o Mg e o K trocáveis, o P disponível, o carbono orgânico, o teor de umidade e a colonização radicular por micorrizas, e diminuiu o Al trocável e a biomassa microbiana. Foram considerados como causas da melhoria da fertilidade do solo, sob cafeeiros adensados, o adequado controle da erosão, o correto manejo dos resíduos vegetais e a baixa lixiviação dos íons. O manejo da população de cafeeiros pode

  13. Zoneamento agroclimático da cultura do café para a Bacia do Rio Doce Agriculture-climatological zoning of coffee crop for the Rio Doce Basin

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    Edson Luís Nunes

    2007-12-01

    Full Text Available Objetivou-se neste trabalho, através da geoespacialização, a identificação das regiões aptas, restritas e inaptas ao cultivo do café (Coffea arabica L. na Bacia do Rio Doce. Utilizou-se para tal, dados de temperatura e déficit hídrico de 50 estações meteorológicas instaladas na bacia e em bacias limítrofes. Os dados de déficit hídrico foram determinados utilizando o balanço hídrico segundo Thornthwaite & Mather (1955. Foram identificadas regiões equivalentes a um terço da bacia, localizadas na parte central e no nordeste da mesma, como sendo inaptas ao cultivo do café, conforme os critérios de produtividade relacionados com as exigências térmicas e hídricas da cultura.It was aimed in this work, through the geospatialization, the identification of the apt, restricted and inapt regions for cultivation of coffee (Coffea arabica L. in Rio Doce watershed. It was used for such, temperature and water deficit data of 50 meteorological stations installed in the watershed and in bordering watershed. The data of water deficit were determined using the water balance according to Thornthwaite & Mather (1955. They were identified equivalent regions the one third of the watershed, located in the central part and in the northeast of the same, as being inapt to the cultivation of coffee, according to the productivity criterions related with the thermal and hydric demands of culture.

  14. coffea arabica l. cvs. caturra and catuaí

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    Andrés M. Gatica

    2007-01-01

    Full Text Available La presente investigación tuvo como objetivo establecer una metodología para la inducción de la embriogénesis somática directa en las variedades de café Caturra y Catuaí. Se observó un efecto del genotipo en la inducción de la embriogénesis somática directa; con la variedad Caturra se obtuvo un mayor número de embriones somáticos (2,71±0,45. Los explantes de Caturra provenientes de plantas de 3 meses mostraron una mejor respuesta a la inducción de la embriogénesis somática directa que los explantes provenientes de plantas de 12 meses. De los explantes provenientes de plantas de 12 meses de Catuaí rojo no se obtuvo embriones somáticos en ninguno de los medios de cultivo evaluados. No hubo diferencias significativas en el número de embriones somáticos producidos a partir de la primera y segunda hoja de vitroplantas y los explantes provenientes de la parte distal, media y basal de las hojas de plantas de café. El medio de cultivo Yasuda resultó el más indicado para inducir la embriogénesis somática y el desarrollo de embriones somáticos en plántulas en Caturra. Mientras que para Catuaí rojo, fue el de Hatanaka.

  15. In vitro propagation of the new disease resistant Coffea arabica ...

    African Journals Online (AJOL)

    SAM

    2014-06-11

    Jun 11, 2014 ... 3World Agroforestry Centre (ICRAF), Cote d' Ivoire Country Program Cocody Mermoz, 08 BP 2823, ... materials, tissue culture offers a feasible alternative to ... was followed by surface sterilization using 25% of a commercial.

  16. Soil quality under two different management schemes in coffee plantations of southern Colombia.

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    Oscar Eduardo Valbuena-Calderón

    2016-12-01

    Full Text Available The aim of this work was to develop an additive soil quality index (ASQI in agrofostery managements of coffee (Coffea arabica L.. The study took place under two intense and traditional management schemes, in nine farms (32 lots in the south of Colombia, during 2013. A separation of means analysis was held through the LSD Fisher test (P<0,05 to each of the physical and chemical variables of the soil. The variables that showed differences between the schemes were submitted to a main components analysis to select the minimum data set (MDS of the components that explained the most variability and the redundancy was veri ed within the indicators, based on the correlation. The ASQI was obtained from the total sum of soil quality index (SQI of all the indicators, taking into account that the higher the score of the ASQI, the higher the quality of the soil within the study system. The selected physical variables were the content of sand and clay; while the chemical variables were: organic carbon (OC, P, CA, Mg, total bases (TB and Ca/Mg. The best ASQI was obtained from traditional management, because the value of the selected variables matched in a bigger proportion with the quality objective identi ed for the ASQI quanti cation; in this case the crop yield, based on limit values for coffee plantations.

  17. Acúmulo de nutrientes em frutos de cafeeiro em duas altitudes de cultivo: micronutrientes Nutrient accumulation in coffee fruits at two at two plantation altitudes: micronutrients

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    Bruno Galvêas Laviola

    2007-12-01

    Full Text Available Dado à importância de se conhecer a exportação de micronutrientes pelos frutos, bem como, as épocas em que são mais demandados pelo cafeeiro, estudou-se o acúmulo de B, Cu, Fe, Mn e Zn em frutos de Coffea arabica L da antese à maturação, em lavouras estabelecidas em duas altitudes. Estudou-se também a variação no teor desses elementos. Estudou-se o acúmulo de B, Cu, Fe, Mn e Zn em frutos de cafeeiro arábico da antese à maturação em duas altitudes, bem como a variação na concentração dos elementos em folhas dos ramos produtivos. O experimento foi constituído da variedade de cafeeiro (Coffea arabica L. Catuaí IAC 44 cultivada a 720 e 950 m de altitude, no município de Martins Soares-MG. O delineamento experimental foi inteiramente ao acaso, com três repetições, usando um esquema de parcela subdividida no tempo. O aumento da altitude influenciou o ciclo reprodutivo do cafeeiro, demandando maior tempo para formação dos frutos. O consumo de nutrientes pelos frutos, assim como o enchimento de grãos, foi mais crítico em condições de menor altitude, já que a planta necessitou completar esses processos em menor espaço de tempo. No estádio de expansão rápida, a percentagem de acúmulo de micronutrientes foi maior na altitude de 720 m, comparada à de 950 m. De modo geral, a altitude influenciou a variação das concentrações foliares de nutrientes, apesar de não se ter observado resposta-padrão da concentração foliar ao aumento da altitude. Conclui-se que a altitude teve influência na extensão do ciclo, bem como no acúmulo de micronutrientes em frutos e na variação, das concentrações foliares destes elementos em folhas de cafeeiro.In view of the importance of knowing fruit micronutrients export from the soil, and the season in which its coffee-plant demand is higher, the accumulation of B, Cu, Fe, Mn and Zn in fruits as well as the variation in the leaf content of the elements in productive branches of

  18. AFLP analysis among Ethiopian arabica coffee genotypes

    African Journals Online (AJOL)

    STORAGESEVER

    2008-09-17

    Sep 17, 2008 ... AFLP analysis. AFLP analysis was performed following the protocol of Vos et al. ... 72oC. All polymerase chain reactions (PCR) were perform- ... ACT/MseI-CAG showed the highest value for MI ..... WL, Lee M, Porter K (2000).

  19. Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties.

    Science.gov (United States)

    Ogita, Shinjiro; Uefuji, Hirotaka; Morimoto, Masayuki; Sano, Hiroshi

    2004-04-01

    The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all corresponding cDNAs designated as CaXMT1 , CaMXMT1 , CaMXMT2 and CaDXMT1 , respectively, and showed that caffeine was indeed synthesized in vitro by the combination of their gene products. In order to regulate caffeine biosynthesis in planta , we suppressed expression of CaMXMT1 by the double stranded RNA interference (RNAi) method. For this purpose, we first established a protocol for efficient somatic embryogenesis of Coffea arabica and C. canephora , and then Agrobacterium -mediated transformation techniques. The RNAi transgenic lines of embryogenic tissues derived from C. arabica and transgenic plantlets of C. canephora demonstrated a clear reduction in transcripts for CaMXMT1 in comparison with the control plants. Transcripts for CaXMT1 and CaDXMT1 were also reduced in the most cases. Both embryonic tissues and plantlets exhibited a concomitant reduction of theobromine and caffeine contents to a range between 30% and 50% of that of the control. These results suggest that the CaMXMT1 -RNAi sequence affected expression of not only CaMXMT1 itself, but also CaXMT1 and CaDXMT1 , and that, since the reduction in theobromine content was proportional to that for caffeine, it is involved in the major synthetic pathway in coffee plants. The results also indicate that the method can be practically applied to produce decaffeinated coffee plants.

  20. Produtividade do cafeeiro Mundo Novo enxertado e submetido à adubação verde antes e após recepa da lavoura Productivity of grafted coffee during intercropping with five leguminous species in the western region of São Paulo State, Brazil

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    Edison Martins Paulo

    2006-01-01

    Full Text Available Estudaram-se a produção e o crescimento do cafeeiro Mundo Novo (Coffea arabica L. enxertado sobre o Apoatã IAC 2258 (Coffea canephora Pierre ex Froehner submetido à adubação verde com as seguintes espécies leguminosas: crotalária espectábilis (Crotalaria spectabilis Roth., crotalária júncea (Crotalaria juncea L., guandu [Cajanus cajan (L. Millsp.], mucuna anã (Stizolobium deeringeanum Bort. e soja IAC 9 [Glycine max (L. Merril] e um tratamento testemunha sem plantas leguminosas. As leguminosas foram semeadas a 50 cm da projeção da copa dos cafeeiros e incorporadas no florescimento. O experimento foi desenvolvido no Pólo Regional de Desenvolvimento Tecnológico dos Agronegócios da Alta Paulista, em Adamantina, no período de 1989 a 1995. Adotou-se o delineamento estatístico de blocos ao acaso com seis tratamentos e cinco repetições. Os adubos verdes crotalária espectábilis, crotalária júncea, mucuna anã e soja, durante o período experimental, e a crotalária espectábilis após a recepa, não diminuíram a produção do cafeeiro. O guandu, embora tenha aumentado o teor de matéria orgânica do solo, foi a única leguminosa que diminuiu a produção e o diâmetro do caule dos cafeeiros. O guandu e a crotalária júncea, respectivamente, produziram as maiores quantidades de fitomassa seca. A produção do café se correlacionou inversamente com a fitomassa seca das leguminosas e positivamente com altura e diâmetro do caule do cafeeiro.Yield of arabica coffee (Coffea arabica of grafted onto robusta coffee (Coffea canephora Pierre ex Froehner Apoatã IAC 2258 was evaluated during six years of intercropping with five leguminous species: sunn hemp (Crotalaria juncea L., Crotalaria spectabilis Roth., dwarf velvet bean (Stizolobium deeringeanum Bort., soybean Glycine max (L. Merryl] and pigeon pea [Cajanus cajan (L. Millsp.] in the Western region of São Paulo State, Brazil, from 1989 to 1995. Leguminous species were sown 50 cm

  1. Compositional changes of proteins and amino acids in germinating coffee seeds

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    Milton Massao Shimizu

    2000-01-01

    Full Text Available Endosperm is the main reserve tissue in coffee seeds. Coffee (Coffea arabica L. seeds were germinated for six weeks and qualitative and quantitative changes in amino acids and proteins were investigated. The total content of free amino acids were reduced during germination, however, protein content remained constant. SDS-PAGE profiles showed that legumin-like proteins became less stained in the last weeks. Asparagine, glutamic acid, aspartic acid, alanine and lysine were the major free amino acids, although serine and glutamine were also significant. Except for tyrosine, which increased with germination, all other amino acids were reduced. Analysis of the amino acid composition of the total soluble protein showed glutamic acid/glutamine and glycine as the main amino acids. However, other amino acids such as leucine, aspartic acid/asparagine, alanine, lysine, serine were also found in reasonable amounts.Endosperma é o principal tecido de reserva em sementes de café. Sementes de café (Coffea arabica L. foram germinadas por seis semanas e as alterações qualitativas e quantitativas de aminoácidos e proteínas foram investigadas. O conteúdo total de aminoácidos livres reduziu durante a germinação, no entanto, o conteúdo de proteínas permaneceu constante. Perfis eletroforéticos de proteínas em SDS-PAGE mostraram que proteínas do tipo legumina foram menos coradas nas últimas semanas. Asparagina, ácido glutâmico, ácido aspártico, alanina e lisina foram os principais aminoácidos, apesar de que serina e glutamina também estavam presentes em quantidades significativas. Exceto tirosina, a qual aumentou durante a germinação, todos os outros aminoácidos tiveram redução em sua concentração. A análise aminoacídica da fração de proteína solúvel total mostrou que ácido glutâmico/glutamina e glicina eram os principais aminoácidos presentes. No entanto, outros aminoácidos, tais como leucina, ácido asp

  2. Root biomass, turnover and net primary productivity of a coffee agroforestry system in Costa Rica: effects of soil depth, shade trees, distance to row and coffee age.

    Science.gov (United States)

    Defrenet, Elsa; Roupsard, Olivier; Van den Meersche, Karel; Charbonnier, Fabien; Pastor Pérez-Molina, Junior; Khac, Emmanuelle; Prieto, Iván; Stokes, Alexia; Roumet, Catherine; Rapidel, Bruno; de Melo Virginio Filho, Elias; Vargas, Victor J; Robelo, Diego; Barquero, Alejandra; Jourdan, Christophe

    2016-08-21

    In Costa Rica, coffee (Coffea arabica) plants are often grown in agroforests. However, it is not known if shade-inducing trees reduce coffee plant biomass through root competition, and hence alter overall net primary productivity (NPP). We estimated biomass and NPP at the stand level, taking into account deep roots and the position of plants with regard to trees. Stem growth and root biomass, turnover and decomposition were measured in mixed coffee/tree (Erythrina poeppigiana) plantations. Growth ring width and number at the stem base were estimated along with stem basal area on a range of plant sizes. Root biomass and fine root density were measured in trenches to a depth of 4 m. To take into account the below-ground heterogeneity of the agroforestry system, fine root turnover was measured by sequential soil coring (to a depth of 30 cm) over 1 year and at different locations (in full sun or under trees and in rows/inter-rows). Allometric relationships were used to calculate NPP of perennial components, which was then scaled up to the stand level. Annual ring width at the stem base increased up to 2·5 mm yr -1 with plant age (over a 44-year period). Nearly all (92 %) coffee root biomass was located in the top 1·5 m, and only 8 % from 1·5 m to a depth of 4 m. Perennial woody root biomass was 16 t ha -1 and NPP of perennial roots was 1·3 t ha -1 yr -1 Fine root biomass (0-30 cm) was two-fold higher in the row compared with between rows. Fine root biomass was 2·29 t ha -1 (12 % of total root biomass) and NPP of fine roots was 2·96 t ha -1 yr -1 (69 % of total root NPP). Fine root turnover was 1·3 yr -1 and lifespan was 0·8 years. Coffee root systems comprised 49 % of the total plant biomass; such a high ratio is possibly a consequence of shoot pruning. There was no significant effect of trees on coffee fine root biomass, suggesting that coffee root systems are very competitive in the topsoil. © The Author 2016. Published by Oxford University Press on

  3. Pengaruh Bionematisida Berbahan Aktif Jamur Paecilomyces lilacinus Strain 251 terhadap Serangan Pratylenchus coffeae pada Kopi Robusta

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    Soekadar Wiryadiputra

    2002-07-01

    Full Text Available Experiment on the effect of P. lilacinus on the infestation of P. coffeae on robusta coffee was conducted in Sumber Asin Experimental Garden, Malang. The treatments were dosages of bionematicide i.e. : 0 (control, 0.25, 0.50, 1.00, 2.00, and 4.00 g; carbofuran (3 % active ingredient 50 g/plant and organic soil treatment (OST at 100 g/plant. Each treatment was replicated four times, and each replication consists of five coffee trees. The results in second year observation revealed that the population of P. coffeae in the roots on PL 251 treatments was not significantly different compared to the control, whereas in soil samples the population of both P. coffeae and Rotylenchulus reniformis inclined to be lower than the control, although they were not statistically significant. The lowest infestation was observed on PL 251 treatment at a dosage level of 4.00 g/tree. On nematode infestation, no significant difference on treatments of carbofuran and OST compared to the control. The yield of green coffee (market coffee was the highest on the treatment of PL 251 at a dosage of 4.00 g/tree and significantly higher than the control and carbofuran treatments, with increasing levels of 225.3 and 198.9%, respectively. Keywords: bionematicide Paecilomyces lilacinus strain 251 (PL 251, Pratylenchus coffeae, Rotylenchulus reniformis

  4. Efecto de la dieta artificial MP sobre la emergencia y relacion de sexos de Phymastichus coffea (Hymenoptera:Eulophidae) mantenido sobre su hueped, Hypothenemus hampei (Coleoptera: Scloytidae)a traves de generaciones contin

    Science.gov (United States)

    Phymastichus coffea La Salle (Hymenoptera: Eulophidae) is an endoparasitoid that attacks the adult coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Scolytidae). The MP diet developed by Portilla and Streett is the only reported diet that allows cultures of P. coffea to develop and repr...

  5. Caracterização de lavouras cafeeiras cultivadas sob o sistema orgânico no sul de Minas Gerais Characterization of coffee crops cultivated on organic system in the south of Minas Gerais

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    Marcelo Ribeiro Malta

    2008-10-01

    Full Text Available As exigências do mercado por cafés de melhor qualidade estão sendo responsáveis pela difusão e adoção de novas tecnologias de produção e preparo de café. Entre os cafés especiais, o café orgânico é um dos que mais vem se destacando neste segmento. Desta forma, objetivou-se nesse trabalho, a avaliação das características agronômicas de lavouras cafeeiras (Coffea arabica L. sob o sistema orgânico de produção, localizadas no Município de Poço Fundo, sul de Minas Gerais. Foram caracterizadas em 21 lavouras cafeeiras orgânicas as cultivares utilizadas, a população cafeeira, o tipo de colheita e secagem adotadas, a produtividade e qualidade do café, a fertilidade do solo e o estado nutricional do cafeeiro. Através dos resultados obtidos, concluiu-se que os cafeeiros conduzidos sob o sistema orgânico de produção apresentam potencial para produzirem cafés de boa qualidade. Em relação à fertilidade do solo, os baixos valores de pH e o desequilíbrio das relações entre K, Ca e Mg observados na maioria dessas lavouras, sugerem que esses fatores podem afetar o crescimento, o desenvolvimento e a produção dos cafeeiros submetidos ao manejo orgânico do sul de Minas Gerais.The market demands for coffee of better quality are being responsible for diffusion and adoption of new production technologies and coffee preparation. Among the special coffee, the organic coffee is one of the most important in this group. So, this paper had the aim of evaluating the agronomical traits of coffee crops (Coffea arabica L. on organic system production, located in Poço Fundo district, South of Minas Gerais. One obtained information about the management of 21 organic coffee crops, like: cultivars, coffee tree population, harvest and drying adopted systems, yield and coffee quality, soil fertility and coffee nutritional status. Based on the obtained results, it is possible to conclude that coffee trees conducted under organic system

  6. Recent advances in coffee berry disease (CBD) control in Uganda

    African Journals Online (AJOL)

    Waller & Bridge) attacks arabic a coffee in most African arabica coffee growing countries. The disease was first recorded in Uganda in 1959 and surveys on the disease indicated that up to 50% crop losses were being incurred. Most of the ...

  7. Morphometric and Productive Characterization of Nineteen Genotypes from the Colombian Coffea Collection / Caracterización Morfométrica y Productiva de Diecinueve Genotipos de la Colección Colombiana del Género Coffea

    Directory of Open Access Journals (Sweden)

    Jhon Wilson Mejía Montoya

    2013-12-01

    Full Text Available Nineteen genotypes of the Colombian Coffee (Coffeaarabica L. Collection were characterized through features related to productivity, crown architecture and light interception. The results revealed significant differences among genotypes. Branches and leaves were found to be dominantly plagiophyl. Leaf area (LA and Leaf area index (LAI made accession CU1812 (which correspondsto variety Castillo® stand out for its photosynthetically activeradiation (PAR interception and coffee bean production. Likewise, a PAR based cluster analysis allowed dividing the genotypes in three groups. Because of their higher yield, the most outstanding genotypes were Caturra, CU-1812 and Harrar R2. This factor showed correlation with PAR absorption. The current results are useful for future works in coffee breeding programs. / Se caracterizaron 19 genotipos de la Colección Colombiana de Café (Coffea arabica L.; para ello se estudiaron aspectos relacionados con la arquitectura del dosel, la interceptación de la radiación y la producción. Los resultados mostraron diferencias significativas entre los genotipos, predominando en las ramas la distribución plagiofila; una disposición similar se observóen las hojas. El área foliar (AF y el índice de área foliar (IAF,contribuyeron a que la introducción CU1812, componente de la variedad Castillo®, se destacara por presentar los mayores valores de interceptación de radiación fotosintéticamente activa (RFA y producción de café cereza. Así mismo, el análisis de agrupamiento con base en la RFA, permitió ordenar los genotipos en tres grupos,destacándose Caturra, CU-1812 y Harrar R2 por su mayorrendimiento, factor que mostró correlación con la RFA captadapor el dosel. Los resultados obtenidos en esta investigación son de utilidad en futuros trabajos de mejoramiento genético en café.

  8. Los Chronica Byzantia-Arabica

    Directory of Open Access Journals (Sweden)

    Jose Carlos MARTÍN

    2006-08-01

    Full Text Available Datation (743-744, contextualisation et traduction en espagnol de la Chronica byzantia-arabica.Fechación (743-744, contextualización y traducción al español de la Chronica byzantia-arabica.es

  9. Komunitas Nematoda pada Tanaman Kopi (Coffea Canephora Var. Robusta Muda di Kabupaten Tanggamus Lampung

    Directory of Open Access Journals (Sweden)

    I GEDE SWIBAWA

    2015-09-01

    Full Text Available The Community of Nematode in The Young Coffee ( Coffea Canephora Var. Robusta Crops in Tanggamus District, Lampung. Tanggamus district is one of coffee production center in Lampung province. Since year of 2013, farmers in Tanggamus have been replaced the unproductive old coffee by coffee seed introduced from East Java. Introducing coffe seed from outside area at risk of carrying plant parasitic nematodes. The purpose of this research was to study community of nematode associated with young coffee crops in Tanggamus. Survey was conducted in coffee robusta (Coffea canephora var. robusta fields belonging to farmer on September 2014. Soil samples were collected from three sites: Margo Mulyo, Sumber Rejo and Batu Bedil. Nematodes were extracted by sieving and centrifugation with sugar solution method. The results show that were 20 genera consisted of 9 genera of plant parasitic and 11 genera of free living nematodes associated with young coffee in Tanggamus. The nematode community was dominated by Pratylenchus and Radopholus. The population of Pratylenchus and Radopholus in Sumber Rejo site were 421 and 846 individual per 300 ml of soil respectively. It was needed to indentify up to species taxonomic level for Pratylenchus and Radopholus associated with young coffee in Tanggamus.

  10. Ecosystem Service of Shade Trees on Nutrient Cycling and Productivity of Coffee Agro-ecosystems

    Directory of Open Access Journals (Sweden)

    Rusdi Evizal

    2009-05-01

    Full Text Available Shade trees are significant in certification scheme of sustainable coffee production. They play an importance role on ecosystem functioning. This research is aimed to study ecosystem service of shade trees in some coffee agro-ecosystems particularly on nutrient cycling and land productivity. Four agro-ecosys tems of Robusta coffee (Coffea canephora, namely sun coffee (without shade trees, coffee shaded by Michelia champaca, coffee shaded by Gliricidia sepium, and coffee shaded by Erythrina indica are evaluated during 2007—2008. Smallholder coffee plantation in Sumberjaya Subdistrict, West Lampung, which managed under local standard were employed using Randomized Complete Block Design with 3 replications. The result showed that litter fall dynamic from shade trees and from coffee trees was influenced by rainfall. Shade trees decreased weed biomass while increased litter fall production. In dry season, shade trees decreased litter fall from coffee shaded by M. champaca. G. sepium and E. indica shaded coffee showed higher yield than sun coffee and M. champaca shaded coffee. Except for M. champaca shaded coffee, yield had positive correlation (r = 0.99 with litter fall production and had negative correlation (r = —0.82 with weed biomass production. Biomass production (litter fall + weed of sun coffee and shaded coffee was not significantly different. Litter fall of shade trees had significance on nutrient cycle mainly to balance the lost of nitrogen in coffee bean harvesting.Key Words: Coffea canephora, Michelia champaca, Gliricidia sepium, Erythrina indica, litter production, nutrient cycle, coffee yield.

  11. Bioprospecting endophytic bacteria for biological control of coffee leaf rust Bioprospecção de bactérias endofíticas como agentes de biocontrole da ferrugem do cafeeiro

    Directory of Open Access Journals (Sweden)

    Humberto Franco Shiomi

    2006-02-01

    Full Text Available Suppression of plant diseases due to the action of endophytic microorganisms has been demonstrated in several pathosystems. Experiments under controlled conditions involving endophytic bacteria isolated from leaves and branches of Coffea arabica L and Coffea robusta L were conducted with the objective of evaluating the inhibition of germination of Hemileia vastatrix Berk. & Br., race II, urediniospores and the control of coffee leaf rust development in tests with leaf discs, detached leaves, and on potted seedling of cv. Mundo Novo. The endophytic bacterial isolates tested proved to be effective in inhibiting urediniospore germination and/or rust development, with values above 50%, although the results obtained in urediniospore germination tests were inferior to the treatment with fungicide propiconazole. Endophytic isolates TG4-Ia, TF2-IIc, TF9-Ia, TG11-IIa, and TF7-IIa, demonstrated better coffee leaf rust control in leaf discs, detached leaves, and coffee plant tests. The endophytic isolates TG4-Ia and TF9-Ia were identified as Bacillus lentimorbus Dutky and Bacillus cereus Frank. & Frank., respectively. Some endophytic bacterial isolates were effective in controlling the coffee leaf rust, although some increased the severity of the disease. Even though a relatively small number of endophytic bacteria were tested, promising results were obtained regarding the efficiency of coffee leaf rust biocontrol. These selected agents appears to be an alternative for future replacement of chemical fungicide.Supressão de doenças de plantas por microrganismos endofíticos tem sido demonstrada em diversos patossistemas. Neste trabalho foram selecionados isolados de bactérias endofíticas de folhas e ramos de cafeeiro com potencial para o controle biológico da ferrugem do cafeeiro, pois é conhecido que esses microrganismos podem possuir essa característica. Bactérias endofíticas isoladas previamente de folhas e ramos de Coffea arabica L e Coffea

  12. Anatomical changes on coffee leaves infected by Pseudomonas syringae pv. garcae

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    Lucas Mateus Rivero Rodrigues

    2015-12-01

    Full Text Available ABSTRACTAlthough poorly studied, the bacterial halo blight is an important disease in the major coffee-producing states of Brazil. External damage and anatomical changes on leaves were measured in seedlings of Coffea arabica cv. Mundo Novo, susceptible to Pseudomonas syringae pv. garcae, by using histological sections obtained at 10 and 20 days after inoculation (DAI. The changes on the epidermis were smaller than the lesions measured in the mesophyll, irrespective of the evaluated colonization period, showing that the internal damage caused by the bacterium represent twice the damage observed externally. From the inoculation site, lysis occurred on the epidermal cells and on the palisade and spongy parenchyma cells, with strong staining of their cellular contents, as well as abnormal intercellular spaces in the palisade parenchyma, hypertrophy and hyperplasia of mesophyll cells and partial destruction of chloroplasts. Additionally, this study revealed the presence of inclusion bodies in epidermal and mesophyll cells. Bacterial masses were found in the apoplast between and within mesophyll cells. Bacteria were also observed in the bundle sheath and vascular bundles and were more pronounced at 20 DAI, not only near the inoculation site but also in distant areas, suggesting displacement through the vascular system. These results can be useful to understand this plant-pathogen interaction.

  13. Multivariate analysis and geostatistics of the fertility of a humic rhodic hapludox under coffee cultivation

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    Samuel de Assis Silva

    2012-04-01

    Full Text Available The spatial variability of soil and plant properties exerts great influence on the yeld of agricultural crops. This study analyzed the spatial variability of the fertility of a Humic Rhodic Hapludox with Arabic coffee, using principal component analysis, cluster analysis and geostatistics in combination. The experiment was carried out in an area under Coffea arabica L., variety Catucai 20/15 - 479. The soil was sampled at a depth 0.20 m, at 50 points of a sampling grid. The following chemical properties were determined: P, K+, Ca2+, Mg2+, Na+, S, Al3+, pH, H + Al, SB, t, T, V, m, OM, Na saturation index (SSI, remaining phosphorus (P-rem, and micronutrients (Zn, Fe, Mn, Cu and B. The data were analyzed with descriptive statistics, followed by principal component and cluster analyses. Geostatistics were used to check and quantify the degree of spatial dependence of properties, represented by principal components. The principal component analysis allowed a dimensional reduction of the problem, providing interpretable components, with little information loss. Despite the characteristic information loss of principal component analysis, the combination of this technique with geostatistical analysis was efficient for the quantification and determination of the structure of spatial dependence of soil fertility. In general, the availability of soil mineral nutrients was low and the levels of acidity and exchangeable Al were high.

  14. Overview of the functional virulent genome of the coffee leaf rust pathogen Hemileia vastatrix with an emphasis on early stages of infection

    Directory of Open Access Journals (Sweden)

    Pedro eTalhinhas

    2014-03-01

    Full Text Available Hemileia vastatrix is the causal agent of coffee leaf rust, the most important disease of coffee (Coffea arabica. In this work, a 454-pyrosequencing transcriptome analysis of H. vastatrix germinating urediniospores (gU and appressoria (Ap was performed and compared to previously published in planta haustoria-rich (H data. A total of 9234 transcripts were identified and annotated. Ca. 50% of these transcripts showed no significant homology to international databases. Only 784 sequences were shared by the three conditions, and 75% were exclusive of either gU (2146, Ap (1479 or H (3270. Relative transcript abundance and RT-qPCR analyses for a selection of genes indicated a particularly active metabolism, translational activity and production of new structures in the appressoria and intense signalling, transport, secretory activity and cellular multiplication in the germinating urediniospores, suggesting the onset of a plant-fungus dialogue as early as at the germ tube stage. Gene expression related to the production of carbohydrate-active enzymes and accumulation of glycerol in germinating urediniospores and appressoria suggests that combined lytic and physical mechanisms are involved in appressoria-mediated penetration. Besides contributing to the characterisation of molecular processes leading to appressoria-mediated infection by rust fungi, these results point towards the identification of new H. vastatrix candidate virulence factors, with 516 genes predicted to encode secreted proteins.

  15. Estudo cariométrico dos poliplóides de Coffea

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    Giorgio Schreiber

    1946-01-01

    Full Text Available Foi estudado o volume nuclear de uma série de plantas de café com 22-44-66-88 cromossômios: C. congensis, C. arabica var. monosperma, C. arabica var. typica e C. arabica var. bullata e pelo método estatístico cariométrico. Das curvas de frequência (que não são "normais", foi calculado o valor normal (modo para cada número de cromossômios. Com êstes valores modais e os respectivos números de cromossômios foi calculada a equação de regressão, o êrro de estimação e o coeficiente de correlação. A correlação é perfeita, sendo, porém, a espécie C. congensis discordante dos demais elementos da série por ter um valor volumétrico correspondente a 33 cromossômios apesar dos 22 encontrados nas metáfases somáticas. Êste resultado é apontado para ulteriores investigações citogenéticas sôbre esta espécie. O resultado geral destas pesquisas mostra a perfeita correlação entre o número de genomas haplóides e o volume nuclear ; deve ser considerado, porém, válido somente no confronto entre núcleos que se encontram na mesma fase do ciclo de crescimento interfásico.Caryometric researches on Coffea polyploids. General discussion of the problem and preliminary results. A series of polyploid plant of Coffea with 22, 44, 66, and 88 chromosomes (C. congensis, C. arabica var. monosperma, C. arabica var. typica, C. arabica var. bullata have been studied with caryometric statistical method. The chromosome numbers have been plotted again,st the modal value of the frequency curves of nuclear volume and the regression equation, standard error of estimation and correlation coefficent have been calculated. The correlation results perfect between the chromosome number and the nuclear volume for all the elements of the polyploid series of Coffea arabica. Coffea congensis, althought with 22 chromosomes, has a nuclear volume exactly correspondent to that of C. arabica with 33 chromosomes. This fact is appointed for further

  16. Efeitos de águas residuárias de café no crescimento vegetativo de cafeeiros em seu primeiro ano Effects of processing coffee wastewater on first year coffee plants growth

    Directory of Open Access Journals (Sweden)

    Moisés S. Ribeiro

    2009-01-01

    Full Text Available Ao utilizar o solo como forma de tratamento e de disposição final de águas residuárias de café (ARC e essa como fonte de água e de nutrientes para os cafeeiros, solucionam-se problemas ambientais e diminuem-se os custos de produção para o cafeicultor. Este trabalho teve por objetivo avaliar os efeitos de diferentes doses de potássio aplicadas via ARC no crescimento vegetativo de cafeeiros (Coffea arabica L., Catuaí' e Catucaí', em seu primeiro ano, bem como o incremento de potássio e a condutividade elétrica na solução do solo. O estudo foi desenvolvido em casa de vegetação do Departamento de Engenharia, na Universidade Federal de Lavras, no município de Lavras - MG. Os tratamentos foram definidos por diferentes doses de potássio (K+: 30 mg L-1; 65 mg L-1; 100 mg L-1; 135 mg L-1, aplicadas via ARC, e pela testemunha (água de irrigação e adubação convencional. ARC com concentrações até 135 mg L-1 de potássio contribui para o crescimento vegetativo do cafeeiro, especificamente, altura de planta e diâmetro do ramo ortotrópico, mostrando-se igualmente, ou melhor, que a água de irrigação e potássio por meio da adubação convencional. Além disso, o aumento na concentração de potássio do solo em todos os tratamentos não foi suficiente para aumentar a condutividade elétrica em níveis que possam afetar o crescimento da planta, no período avaliado.Using the soil as a form of treatment and final disposal for coffee wastewater(CW, considering its water and nutrient content to the coffee, solves environmental problems and reduces production costs for coffee growers. This research had the objective to evaluate the influence of different potassium levels from WC on the growth of (Coffea arabica L., Catuaí' and Catucaí' coffee varieties, in their fist year, as well the potassium increase and electrical conductivity in the soil solution. The experiment was carried out in a greenhouse of the Engineering Department, of

  17. Study on the determination of coffea arabic radio sensibility curve

    International Nuclear Information System (INIS)

    Robleda, D.; Velazquez, R.

    1993-01-01

    The radiosensitivity curve of Coffea Arabic submitted to gamma irradiation from a 60C o source at the MRX-1500 with a dose power of 105 Gy/min was determined. For that study,. coffee seeds were irradiated at doses of 10,40,80 and 120 Gy, with a 40% of relative humidity; percentage of germination, height decrease, number and size of leaves, number and length of modes. As result of this analysis, GR50 and GR20 doses for searching genetic variability related to plant size reduction

  18. An Evaluation of the Genetic Diversity of Xylella fastidiosa Isolated from Diseased Citrus and Coffee in São Paulo, Brazil.

    Science.gov (United States)

    Qin, X; Miranda, V S; Machado, M A; Lemos, E G; Hartung, J S

    2001-06-01

    ABSTRACT Strains of Xylella fastidiosa, isolated from sweet orange trees (Citrus sinensis) and coffee trees (Coffea arabica) with symptoms of citrus variegated chlorosis and Requeima do Café, respectively, were indistinguishable based on repetitive extragenic palindromic polymerase chain reaction (PCR) and enterobacterial repetitive intergenic consensus PCR assays. These strains were also indistinguishable with a previously described PCR assay that distinguished the citrus strains from all other strains of Xylella fastidiosa. Because we were not able to document any genomic diversity in our collection of Xylella fastidiosa strains isolated from diseased citrus, the observed gradient of increasing disease severity from southern to northern regions of São Paulo State is unlikely due to the presence of significantly different strains of the pathogen in the different regions. When comparisons were made to reference strains of Xylella fastidiosa isolated from other hosts using these methods, four groups were consistently identified consistent with the hosts and regions from which the strains originated: citrus and coffee, grapevine and almond, mulberry, and elm, plum, and oak. Independent results from random amplified polymorphic DNA (RAPD) PCR assays were also consistent with these results; however, two of the primers tested in RAPD-PCR were able to distinguish the coffee and citrus strains. Sequence comparisons of a PCR product amplified from all strains of Xylella fastidiosa confirmed the presence of a CfoI polymorphism that can be used to distinguish the citrus strains from all others. The ability to distinguish Xylella fastidiosa strains from citrus and coffee with a PCR-based assay will be useful in epidemiological and etiological studies of this pathogen.

  19. Ploidy level and caffeine content in leaves of Coffea Ploidia e conteúdo de cafeína em folhas de Coffea

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    Maria Bernadete Silvarolla

    1999-07-01

    Full Text Available Several species of Coffea and varieties of C. arabica differing in chromosome number had their caffeine content determined in the leaves. For Coffea arabica var. "Angustifolia", var. "Caturra" and var. "Icatu", it was observed caffeine decrease from the haploid (2n = 22 to tetraploid leaves (2n = 44. Caffeine in the tetraploid "Angustifolia" was decreased by 50% when compared to the haploid. Caffeine reduction was also observed in leaves of C. canephora var. "Kouilou" as chromosome number was increased (2n = 22 ® 2n = 44. In this case, caffeine in the leaves of the diploid genotype was close to 4 times higher than in the tetraploid. On the other hand it was observed an increase of the alkaloid when the chromosome number was doubled in the C. canephora var. "Robusta".Variedades e espécies de café (Coffea que diferem quanto ao número de cromossomos tiveram o conteúdo de cafeína determinado em suas folhas. Para as variedades "Angustifolia", "Caturra" e "Icatu" de Coffea arabica foi observada redução no teor de cafeína de folhas haplóides (2n = 22 em relação a tetraplóides (2n = 44. Cafeína na forma tetraplóide da var. "Angustifolia" foi reduzida em 50% quando comparada com a forma haplóide. Redução de cafeína também foi observada em folhas de C. canephora var. "Kouilou" quando o número de cromossomos aumentou (2n = 22 ® 2n = 44. Neste caso, a cafeína nas folhas do genótipo diplóide foi cerca de quatro vezes maior que no tetraplóide. Por outro lado, foi observado aumento desse alcalóide quando o número de cromossomos foi duplicado em C. canephora var. "Robusta".

  20. Efeito da bebida de café descascado sobre a atividade antioxidante, os parâmetros hematológicos e bioquímicos em ratos Peeled coffee brew effect in the antioxidant activity hematological and biochemical parameters in rats

    Directory of Open Access Journals (Sweden)

    Stella Maris da Silveira Duarte

    2009-12-01

    Full Text Available O objetivo deste estudo foi verificar o efeito da bebida filtrada, preparada com café-arábica descascado, sobre a peroxidação de lipídios, os parâmetros hematológicos e bioquímicos in vivo. Para isso foram utilizados ratos que ingeriram 280 mg/kg/dia da bebida de café por 7 dias (tratamento agudo e 30 dias (tratamento crônico. A fim de determinar se a bebida de café é capaz de reduzir o estresse oxidativo, foi analisada a peroxidação de lipídios isolados de cérebro de rato analisando-se as substâncias reativas do ácido tiobarbitúrico. A ingestão da bebida por 7 e 30 dias inibiu significativamente a peroxidação lipídica (p The aim of this study was to verify the effect of filtered coffee brew prepared with peeled Coffea arabica on the lipid peroxidation and on hematological and biochemical parameters in vivo. Rats were ingested 280 mg/kg/day of coffee brew for 7 days (acute treatment and 30 days (chronic treatment. In order to determine whether coffee can reduce the oxidative stress, the rats brain isolated lipid peroxidation was accessed evaluating the thiobarbituric acid-reactive substances (TBARs.The ingestion of coffee brew for 7 and 30 days inhibited, significantly, the malondialdehyde concentration (p 0.05 between the percentage of peroxidation inhibition when the acute (48.6% and chronic (53.4% treatments were compared. The chronic ingestion of moderate doses of filtered coffee brew did not modify the plasma level of the hematological and biochemical parameters analyzed. The results indicate the beneficial health effect of moderated filtered coffee brew ingestion since it inhibited lipid peroxidation, but did not change the rats cholesterol and triacylglycerol plasma levels.

  1. Identification by the DArTseq method of the genetic origin of the #Coffea canephora# cultivated in Vietnam and Mexico

    OpenAIRE

    Garavito, Andrea; Montagnon, Christophe; Guyot, Romain; Bertrand, Benoît

    2016-01-01

    Background The coffee species Coffea canephora is commercially identified as ?Conilon? when produced in Brazil, or ?Robusta? when produced elsewhere in the world. It represents approximately 40?% of coffee production worldwide. While the genetic diversity of wild C. canephora has been well studied in the past, only few studies have addressed the genetic diversity of currently cultivated varieties around the globe. Vietnam is the largest Robusta producer in the world, while Mexico is the only ...

  2. Coffea canephora Pierre

    African Journals Online (AJOL)

    Uganda has been hampered by logistics. These clonal ... confirmed that it is highly profitable to apply nitrogen to robusta coffee. The differences ..... cost of in yield over due to response profit"'. /ha/year {kg}"1. Nitrogen zero level {kg) to ...

  3. Effect on light intensity and mineral nutrition on carbohydrate and organic acid content in leaves of young coffee plants

    International Nuclear Information System (INIS)

    Georgiev, G.; Vento, Kh.

    1975-01-01

    Young coffee plants (Coffea arabica, L., var. Caturra) were grown under different conditions of mineral nutrition (1/8 N-P-K, N-P-K, 3 N-P-K, N 1/2-P-K and N-2P-K) and illumination (directly in the sunlight or shaded) with the aim of studying the effect of light and mineral nutrition on carbohydrate and organic acid content of the leaves. For determining these compounds 14 CO 2 was used. Sugars were separated after the method of paper chromatography. The results obtained showed that the incorporation of 14 C in sugars and organic acids was more intensive in plants grown directly in the sunlight, while in starch 14 C was incorporated more intensively in the shaded plants. Carbohydrate content rose parallel to the increase of nitrogen in the nutrient solution. Changingthe rate of phosphorus from 1/2P to two doses exerted highest effect on 14 C incorporation in starch and in hemicellulose. (author)

  4. Farmer awareness, coping mechanisms and economic implications ...

    African Journals Online (AJOL)

    Coffee leaf rust (CLR) still remains a serious threat to the economics of coffee farming in Uganda. The disease is more severe on Arabica coffee (Coffea arabica) at mid and low altitude (1500 m and below) where crop losses is up to 50%. The objective of this study was to document farmers' knowledge about the disease, ...

  5. Irrigação e certificação da cafeicultura na Região Centro-Oeste de São Paulo Irrigation and certification on coffee crop in mid-west of São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    Marcos José Perdoná

    2012-01-01

    Full Text Available Rentabilidade insatisfatória tem provocado diminuição paulatina do parque cafeeiro no Estado de São Paulo. Entre as alternativas para a solução deste problema, duas se destacam: a irrigação das lavouras e a certificação das propriedades. Mesmo em regiões consideradas aptas à cultura do café arábica (Coffea arabica L., o uso da irrigação pode promover, na média dos anos, aumento de produtividade de grãos. Além disso, a certificação do produto favorece sua diferenciação no mercado e pode melhorar os resultados econômicos da atividade. Este trabalho teve por objetivo avaliar o desenvolvimento e a produtividade de cafeeiros submetidos a sistemas irrigados ou não, comparando os dados econômicos na comercialização do café certificado ou não, nas condições da Região Centro-Oeste do Estado de São Paulo. O delineamento utilizado no campo foi o de blocos ao acaso, com dois tratamentos (com e sem irrigação por gotejamento e dez repetições. Foram calculados os custos de produção, bem como a lucratividade com a venda do produto final. A irrigação aumentou o desenvolvimento e a produtividade dos cafeeiros. Houve o retorno do investimento a partir da terceira safra. A venda de café certificado possibilitou resultados econômicos em média de 224,5% superiores ao não certificado. A irrigação aliada à venda de café certificado proporcionou saldo financeiro 1.192% superior ao café não irrigado e não certificado.An unsatisfactory profitability has led to the gradual decline of the coffee plantations in São Paulo. Among the alternatives that arise for solving this problem, two stand out: the irrigation of crops and certification of properties. Even in areas considered suitable for cultivation of arabica coffee (Coffea arabica L., the use of irrigation can promote an increase in grain yield. In addition, the product certification promotes their differentiation in the market and can improve the economic results of

  6. Teor de óleo e de cafeína em variedades de café Oil and caffeine content in the coffee bean

    Directory of Open Access Journals (Sweden)

    J. S. Tango

    1963-01-01

    Full Text Available Determinou-se a porcentagem de óleo e de cafeína em sementes de diversas variedades de Coffee arabica. As sementes provieram de frutos despolpados, secos em terreiro sem despolpar, ou secos na própria planta. O tratamento des frutos influiu sôbre o teor de óleo, sendo também significativa a diferença entre as variedades. Os dados confirmam resultados anteriores de que a variedade mucronata é rica e a, variedade laurina, pobre, em óleo. No que concerne ao teor de cafeína, o tratamento dos frutos não mostrou influência, mas pronunciada diferença se constatou entre as variedades. Sete das variedades se mostraram mais pobres do que o café 'Sumatra', tomado comg padrão. A variedade laurina destacou-se das demais pelo baixo teor dêsse alcalóide com, aproximadamente, a metade do nível encontrado nas demais variedades analisadas, o que a torna valiosa para um piano de melhoramento visando a redução do nível de cafeína nas sementes.Oil and caffeine contents were determined for seed samples obtained from cherries of sixteen varieties of Coffea arabica which were submitted to three types of treatments pulped, non pulped and left to dry with pericarp in the sun, or naturally dried on the tree. Cherry processing seems not to have influence on the oil content in the seeds (table I, but significant differences were noted among the varieties with respect to this characteristic. Mucronata revealed to have high oil content while the laurina is a low oil producing variety. Coffee cherries left to dry on the trees gave lower caffeine content. Seven of the sixteen varieties presented a lower caffeine content than the standard 'Sumatra'. The laurinis variety had an exceptional lower caffeine content of about half the amount found in the control. Laurina characteristics are controlled by a recessive allele lr with strong pleiotropic effects. It is not yet known how the laurina allele affects the chemical reactions which lead to a so low caffeine

  7. Avaliação da suscetibilidade à Xylella fastidiosa em diferentes espécies de cafeeiro Susceptibity valuation to Xylella fastidiosa in different coffee species

    Directory of Open Access Journals (Sweden)

    Rachel Benetti Queiroz-Voltan

    2005-01-01

    Full Text Available A bactéria Xylella fastidiosa Wells et al. foi detectada pela primeira vez em cafeeiro no Brasil, em 1995, entretanto acredita-se que a cultura foi infectada por essa bactéria há muitos anos, embora os sintomas fossem atribuídos a um estresse nutricional. Até o momento têm sido realizados estudos principalmente com espécies de C. arabica e C. canephora, porém, em outras espécies do gênero, somente foi detectada sua presença. Neste trabalho, objetivou-se avaliar a proporção de elementos de vaso do xilema obstruídos, total e parcialmente, pela X. fastidiosa, naturalmente infectadas, em diferentes espécies de cafeeiro do Banco de Germoplasma do IAC, visando identificar material resistente a essa bactéria para ser utilizado no programa de melhoramento genético. Os acessos estudados foram: C. canephora (progenitora da 'Guarini', C. liberica var. liberica, os quatro acessos de C. liberica var. dewevrei (Ugandae, Dibowskii, Abeokutae, Excelsa e o híbrido interespecífico Piatã (C. arabica X C. liberica var. dewevrei. Todos eles mostraram-se menos suscetíveis à X. fastidiosa. A porcentagem de obstrução dos elementos de vasos na folha não foi maior que 0,6% na maioria dos acessos, com exceção de Excelsa e do híbrido Piatã com até 2% de obstrução, sendo bem menos suscetíveis a essa bactéria do que as cultivares de C. arabica. Trata-se, portanto, de materiais genéticos importantes para serem utilizados no programa de melhoramento do cafeeiro visando à resistência ao agente dessa doença.Xylella fastidiosa Wells et al. bacteria was firstly detected in coffee plants in Brazil in 1995. However it is believed to be attacking this crop this time. Disease symptoms have been attributed mostly to nutritional unbalances. Up to date studies have comprised only the species C. arabica and C. canephora. However X. fastidiosa was also detected in other Coffea species, but without disease symptoms. Aiming to identify in the IAC

  8. Estudos histoplásticos da interação Colletotrichum gloeosporioides: cafeeiro Histopathological studies of the interaction Colletotrichum gloeosporioides: coffee tree

    Directory of Open Access Journals (Sweden)

    Igor Souza Pereira

    2009-01-01

    Full Text Available Nos estudos envolvendo a interação Colletotrichum gloeosporioides-cafeeiro (Coffea arabica L., poucas são as informações a respeito do modo de penetração e colonização deste patógeno. Estudou-se por meio da microscopia eletrônica de varredura (MEV, os eventos de pré-penetração e penetração de C. gloeosporioides em hipocótilos de cafeeiros e a colonização natural de ramos e frutos provenientes de plantas com mancha manteigosa. Realizaram-se três ensaios. No primeiro, fez-se a observação dos eventos de pré-penetração de C. gloeosporioides inoculados em hipocótilos de plântulas da cv. Acaiá Cerrado com e sem ferimentos; no segundo, observou-se a colonização de ramos enfermos em que havia murcha drástica e necrose local e, no terceiro, a colonização de frutos enfermos. A germinação dos conídios nos hipocótilos feridos ocorreu 6 horas após a inoculação, com a formação de um ou dois tubos germinativos terminais e a adesão dos conídios nas depressões dos hipocótilos. Apressórios globosos a subglobosos e de contorno regular surgiram 12 horas após a inoculação. Até 72 horas, não foi possível observar a formação de acérvulos sobre os tecidos submetidos à inoculação. Nos ramos observava-se colonização nos vasos floema e parênquima cortical. Nos frutos em diferentes estádios de maturação foi observada a colonização do tecido próximo à epiderme e colapso dos estômatos na área lesionada.In studies involving the interaction Colletotrichum gloeosporioides - coffee trees (Coffea arabica L. there is little information regarding the pre-penetration, penetration and colonization pathways of this pathogen. The objectives of this work were: 1. to study through scanning electron microscopy (SEM the pre-penetration and penetration events of C. gloeosporioides in hypocotyls of coffee plantlets; 2. to observe the colonization of Colletotrichum spp. in branches and fruits of coffee trees with blister

  9. Economic Optimization of Nutrient Application to Coffee in Northern Tanzania Using SAFERNAC

    NARCIS (Netherlands)

    Maro, G.P.; Janssen, B.H.; Msanya, B.M.; Mrema, J.P.

    2014-01-01

    The aim of this work, as an extension to SAFERNAC model, was to establish economically optimum combinations of N, P and K application to Arabica coffee in the Northern coffee zone of Tanzania. The study was conducted in Hai and Lushoto districts between 2010 and 2012. Prices of nutrient inputs and

  10. Modeling of the shrinkage kinetics of coffee berries during drying = Modelagem da cinética de contração de frutos de café durante a secagem

    Directory of Open Access Journals (Sweden)

    Gabriel Henrique Horta Oliveira

    2011-07-01

    Full Text Available The effects of drying conditions on the shrinkage of Coffea arabica L., cultivar Catuai Vermelho, were studied. An exponential model derived from the kinetic model was used to describe the shrinkage kinetics. In addition, an equation was proposed to describe the relationship between the superficial areas and weights of the coffee berries. Coffee berries were dried at temperatures (T of 35, 45, 55 and 65oC and at relative humidities (ƒÓ of 25, 35, 45 and 55%, forming 16 different sets of conditions, with an initial moisturecontent for all conditions of 2.27 dry basis (d.b., dried until 0.11 d.b. Changes in the product dimensions were measured continuously during drying. Surface area-to-volume ratio values increased with moisture content decrease. The model was suitable for representing the shrinkage kinetics, as well the empirical equation for predicting surface area. Temperature had a greater influence over the constant rate of change of the characteristic dimension (k values than ƒÓ. Activation energies were also determined to have magnitudes of 39.31, 37.32, 36.28 and 36.22 kJ mol-1 for relative humidities of 25, 35, 45and 55%, respectively. Neste trabalho foi estudado o efeito das condicoes de secagem sobre a contracao de frutos de cafe (Coffea arabica L., cultivar Catuai Vermelho. Um modelo exponencial derivado do modelo cinetico foi utilizado para descrever a cinetica da contracao. Ademais, uma equacao foi proposta para descrever a relacao entre a area superficial e a massa dos frutos de cafe. Frutos de cafe foram secos as temperaturas de (T de 35, 45, 55 e 65oC, juntamente com umidades relativas (ƒÓ de 25, 35, 45 e 55%, totalizando 16 condicoesdiferentes, com um teor de agua inicial de 2,27 base seca (b.s., secos ate 0,11 b.s. As alteracoes nas dimensoes do produto foram mensuradas continuamente durante a secagem. Os valores de relacao superficie-volume aumentaram com a diminuicao do teor de agua. Omodelo foi adequado para

  11. Flutuação populacional do bicho-mineiro em cultivares de café arábica resistentes à ferrugem Fluctuation of leaf miner population in resistant arabica coffee cultivars to leaf rust

    Directory of Open Access Journals (Sweden)

    Celso Henrique Costa Conceição

    2005-01-01

    Full Text Available A intensidade de infestação pelo bicho-mineiro, Leucoptera coffeella (Guérin-Méneville (Lepidoptera: Lyonetiidae foi investigada nas cultivares Obatã IAC 1669-20 e Tupi IAC 1669-33, com resistência à ferrugem das folhas do cafeeiro, Hemileia vastatrix Berk. et Br., e Ouro Verde Amarelo IAC 4397, suscetível à doença, em ensaios de campo, localizados em Campinas (SP, Brasil. A incidência de ferrugem e a ocorrência de inimigos naturais da praga, assim como o enfolhamento das plantas, foram também observados nas três cultivares. As curvas de flutuação populacional obtidas para Obatã IAC 1669-20 e Tupi IAC 1669-33 revelaram maior incidência do bicho-mineiro entre abril e novembro. Já na cultivar Ouro Verde Amarelo IAC 4397, observaram-se dois picos de infestação, sendo o primeiro em abril-maio e o segundo em agosto-setembro. No entanto, a elevada percentagem de folhas minadas nas cultivares Tupi IAC 1669-33 e Obatã IAC 1669-20 em relação à Ouro Verde Amarelo IAC 4397 não é evidência de maior suscetibilidade à praga, mas sim devido à maior retenção foliar dessas cultivares, em conseqüência da resistência à ferrugem das folhas observada em ambas. De maneira oposta, na cultivar Ouro Verde Amarelo IAC 4397, os sintomas de ataque do bicho-mineiro ocorreram em menor nível especialmente devido a maior queda de folhas. Com base nas diferenças observadas entre as cultivares, sugere-se a adoção de estratégias distintas de manejo da praga.The intensity of infestation of leaf-miner, Leucoptera coffeella (Guérin-Méneville was investigated in coffee cultivars Obatã IAC 1669-20 and Tupi IAC 1669-33, both resistant to the leaf rust agent, Hemileia vastatrix Berk. et Br., and Ouro Verde Amarelo IAC 4397, susceptible to this coffee disease, at field assays in Campinas, SP, Brazil. The incidence of coffee rust and presence of natural enemies, as well as the plant leafiness, were also observed. In Obatã IAC 1669-20 and Tupi

  12. Detecção de fungos micorrízicos arbusculares em raízes de cafeeiro e de crotalária cultivada na entrelinha Detection of arbuscular mycorrhizal fungi in roots of coffee plants and crotalaria cultivated between rows

    Directory of Open Access Journals (Sweden)

    ARNALDO COLOZZI FILHO

    2000-10-01

    Full Text Available Avaliou-se a ocorrência de fungos micorrízicos arbusculares (FMAs no solo rizosférico e nas raízes de cafeeiro (Coffea arabica L. e de Crotalaria breviflora DC., cultivada na entrelinha como adubo verde. Amostras de solo rizosférico e raízes foram coletadas em julho de 1997, em parte de um experimento de longa duração conduzido no campo pelo Instituto Agronômico do Paraná, no município de Mirasselva, PR. Determinou-se a diversidade de FMAs, por meio da identificação morfológica dos esporos, a freqüência de ocorrência de populações de FMAs por meio da contagem direta de esporos no solo, e a colonização radicular. Extraiu-se DNA de raízes de cafeeiro colonizadas e não-colonizadas e de esporos de Acaulospora longula e Scutellospora gilmorei, coletados na rizosfera, realizando-se a PCR ("Polimerase chain reaction" com primers ITS ("Internal transcribed spacer" e comparando os perfis de bandas obtidos. O cultivo de crotalária na entrelinha do cafeeiro aumentou a concentração de esporos de FMAs na rizosfera do cafeeiro. A crotalária e o cafeeiro estimularam populações diferentes de FMAs. O gênero Acaulospora predominou na rizosfera do cafeeiro, e Scutellospora e Gigaspora na rizosfera da crotalária. Usando técnicas moleculares, foi possível caracterizar FMAs na rizosfera e nas raízes colonizadas do cafeeiro. O fungo micorrízico Scutellospora gilmorei, de ocorrência comum em cafeeiro e crotalária, não foi encontrado colonizando as raízes do cafeeiro. O uso de técnicas moleculares pode auxiliar no estudo da dinâmica populacional de FMAs no campo.The sporulation and occurrence of arbuscular mycorrhizal fungi (AMF was evaluated in the coffee trees (Coffea arabica L. and Crotalaria breviflora DC. rhizosphere and roots. C. breviflora was intercropped for green manure of the coffee plants. Samples of rhizosphere soil and roots were collected in July of 1997 in a long-time experiment localized at the Instituto Agron

  13. Drinking Coffee

    DEFF Research Database (Denmark)

    Strøbæk, Pernille Solveig

    2015-01-01

    The chapter explores how coffee is an integral part of our daily life. Focusing on coffee drinking at home, at work, and on the go I show that coffee consumption is a social practice. The chapter illustrates through everyday examples that coffee is more than a caffeine drug. Coffee, with or without...... caffeine, is a social lubricant. We talk to each other and share emotions with one another as we share a cup of coffee. Coffee makes conversation and we embrace coffee, to stay or to go, in the daily rhythm of our busy and global social existence. The practice and sociality of coffee consumption provide...... the coffee industry with the opportunity to make money on our coffee preferences – indeed, also for those of us who actually dislike the taste of coffee. Would you prefer coffee mixed and stirred with non-coffee products such as salt, caramel and licorice? Then you are one of us in the modern age of coffee...

  14. Caracterização morfológica de espécies de Hemerobius Linnaeus, 1758 (Neuroptera, Hemerobiidae associadas a cultivos de café (Coffea arabica L., milho (Zea mays L. e erva-mate (Ilex paraguariensis St. Hill.

    Directory of Open Access Journals (Sweden)

    Lara Rogéria Inês Rosa

    2003-01-01

    Full Text Available The predators were collected in mate crop in Cascavel and São Mateus do Sul, Paraná, Brazil and some other additional specimens in coffee and maize crops in Ribeirão Preto, São Paulo, Brazil. Illustrations obtained by SEM are given by the first time to the principal structures. Three species of Hemerobius were identified: H. bolivari Banks, 1910; H. domingensis Banks, 1941 and H. gaitoi Monserrat, 1996. H. domingensis is recorded for the first time to Brazil.

  15. Study of the energy efficiency of coffee drying with different revolving combinations time; Estudo da eficiencia energetica de secagem de cafe com diferentes combinacoes de tempo de revolvimento

    Energy Technology Data Exchange (ETDEWEB)

    Greco, Marcelo; Campos, Alessandro Torres [Universidade Estadual do Oeste do Parana (UNIOESTE), Marechal Candido Rondon, PR (Brazil). Grupo de Pesquisas em Ambiencia do Oeste do Parana (GPEA)], e-mail: greco.b@bol.com.br; Molin, Roberto Natal dal; Oliveira, Marcos C. Oliveira; Camara, Roberto J; Klein, Jeferson [Universidade Estadual do Oeste do Parana (UNIOESTE), Marechal Candido Rondon, PR (Brazil); Siqueira Filho, Roberto [Universidade Estadual do Oeste do Parana (UNIOESTE), Marechal Candido Rondon, PR (Brazil); Ministerio da Agricultura, Pecuaria e Abastecimento (MAPA), Brasilia, DF (Brazil)

    2004-07-01

    This work objected to evaluate the energy efficiency in the coffee (Coffea arabica L.) drying with different combinations of revolving time in a fixed bed dryer. The experiment was developed the months of June and July of 2003, in a farm located in the municipal district of Jesuitas-Parana State, Brazil. The product quality evaluations were accomplished in the Consolata Agricultural Cooperative Ltda. (COPACOL). The grains parameters were evaluated at the Seeds Technology Laboratory, at the UNIOESTE Campus Marechal Candido Rondon. A fixed bed model dryer was used, with a metallic drying chamber, of cylindrical format, with an adapted grain revolving system, keeping the air temperature at 75 deg C. It was observed that the energy consumed for burning the fuel (wood) overcame the electric energy consumed by the motors. The air relative humidity and temperature exercised influence in the drying time. The combination that provided larger energy efficiency was the continuous ventilation drying, with revolving times of 40 minutes each 2 hours. (author)

  16. Susceptibility of the Parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) to Beauveria bassiana under laboratory conditions; Susceptibilidad del parasitoide Phymastichus coffea LaSalle (Hymenoptera:Eulophidae) a Beauveria bassiana en condiciones de laboratorio

    Energy Technology Data Exchange (ETDEWEB)

    Castillo, Alfredo; Gomez, Jaime; Infante, Francisco [El Colegio de la Frontera Sur (ECOSUR), Chiapas (Mexico). Dept. de Entomologia Tropical], e-mail: acastill@ecosur.mx, e-mail: jgomez@ecosur.mx, e-mail: finfante@ecosur.mx; Vega, Fernando E. [United States Department of Agriculture (USDA), Beltsville, MD (United States). Agricultural Research Service. Sustainable Perennial Crops Lab.], e-mail: fernando.vega@ars.usda.gov

    2009-09-15

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is the most important coffee pest worldwide. Beauveria bassiana is a generalist entomopathogenic fungus widely used by coffee farmers to control this pest and Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) is an African endo parasitoid of H. hampei adults, recently imported to several Latin American and Caribbean countries to aid in the coffee berry borer control. The objective of this study was to determine if B. bassiana is detrimental to P. coffea. The susceptibility of the parasitoid was evaluated in terms of adult survivorship, mean lethal concentration (LC{sub 50}), mean lethal time (LT{sub 50}), reproduction and immature mortality. The main effect of the fungus resulted in reduction of adult longevity and mortality of 100% for immature stages of this parasitoid. The LC{sub 50} for adults was 0.11% equivalent to 9.53 x 10{sup 7} conidia/ml of B. bassiana and a LT{sub 50} of 29.4 h, equivalent to reduction of 22% of its normal longevity as an adult. P. coffea was capable of disseminating spores of B. bassiana to non-infected H. hampei adults, which could indirectly cause the death of its own progeny. These results could be valuable when considering the use of both organisms in the field, especially in an integrated pest management program. (author)

  17. Difference Spectroscopy in the Analysis of the Effects of Coffee Cherry Processing Variables on the Flavor of Brewed Coffee

    International Nuclear Information System (INIS)

    Lyman, D.J.; Benck, R.M.; Merle, S.F.

    2011-01-01

    Infrared difference spectroscopy was used to study how changes in the processing of Arabica coffee cherries into green beans affected the flavor of coffee brewed from roasted green beans. Paired samples of green beans, in which the drying step or fermentation/washing step in their processing was altered, were roasted and brewed in a standard manner and their ATR-FT-IR spectra obtained. Difference spectra of the 1800 to 1680 cm-1 carbonyl region of water-subtracted spectra of paired samples of these brewed coffees provided data which indicated differences in brewed coffee flavor due to changes in fermentation/washing steps and drying steps involved in the processing of coffee cherries. The role of acid, ketone, aldehyde, ester, lactone, and vinyl ester carbonyl components on the flavor of brewed coffee is proposed that is consistent with the flavors as perceived by the coffee tasters.

  18. Difference Spectroscopy in the Analysis of the Effects of Coffee Cherry Processing Variables on the Flavor of Brewed Coffee

    Directory of Open Access Journals (Sweden)

    Donald J. Lyman

    2011-01-01

    Full Text Available Infrared difference spectroscopy was used to study how changes in the processing of Arabica coffee cherries into green beans affected the flavor of coffee brewed from roasted green beans. Paired samples of green beans, in which the drying step or fermentation/washing step in their processing was altered, were roasted and brewed in a standard manner and their ATR-FT-IR spectra obtained. Difference spectra of the 1800 to 1680 cm−1 carbonyl region of water-subtracted spectra of paired samples of these brewed coffees provided data which indicated differences in brewed coffee flavor due to changes in fermentation/washing steps and drying steps involved in the processing of coffee cherries. The role of acid, ketone, aldehyde, ester, lactone, and vinyl ester carbonyl components on the flavor of brewed coffee is proposed that is consistent with the flavors as perceived by the coffee tasters.

  19. Susceptibility of the Parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) to Beauveria bassiana under laboratory conditions

    International Nuclear Information System (INIS)

    Castillo, Alfredo; Gomez, Jaime; Infante, Francisco; Vega, Fernando E.

    2009-01-01

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is the most important coffee pest worldwide. Beauveria bassiana is a generalist entomopathogenic fungus widely used by coffee farmers to control this pest and Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) is an African endo parasitoid of H. hampei adults, recently imported to several Latin American and Caribbean countries to aid in the coffee berry borer control. The objective of this study was to determine if B. bassiana is detrimental to P. coffea. The susceptibility of the parasitoid was evaluated in terms of adult survivorship, mean lethal concentration (LC 50 ), mean lethal time (LT 50 ), reproduction and immature mortality. The main effect of the fungus resulted in reduction of adult longevity and mortality of 100% for immature stages of this parasitoid. The LC 50 for adults was 0.11% equivalent to 9.53 x 10 7 conidia/ml of B. bassiana and a LT 50 of 29.4 h, equivalent to reduction of 22% of its normal longevity as an adult. P. coffea was capable of disseminating spores of B. bassiana to non-infected H. hampei adults, which could indirectly cause the death of its own progeny. These results could be valuable when considering the use of both organisms in the field, especially in an integrated pest management program. (author)

  20. Yield performance and leaf nutrient levels of coffee cultivars under different plant densities Produtividade e níveis foliares de nutrientes em cultivares de café sob diferentes populações de plantas

    Directory of Open Access Journals (Sweden)

    Edison Martins Paulo

    2010-12-01

    Full Text Available Coffee (Coffea Arabica L. plantations using adapted cultivars to regional environmental conditions with optimal plant population density and adequate nutrition are expected to show high yield responses. The triennial production and leaf macronutrient concentrations of four coffee cultivars were studied under different plant population densities. Catuaí Amarelo (IAC 47, Obatã (IAC 1669-20, Acaiá (IAC 474-19 and Icatu Amarelo (IAC 2944 were planted in densities of 2,500; 5,000; 7,519; and 10,000 plants ha-1 with one plant per hole and two plants per hole in the 2,500 plant ha-1. Plants were homogeneously fertilized without liming. As the population density increased the triennial coffee productivity increased, the yield per plant decreased, and leaf concentrations of phosphorus (P, potassium (K and sulfur (S increased. Coffee plants under dense systems presented equal or higher leaf macronutrient concentrations compared to the plants under conventional population. Taller cultivars presented the highest nutrient concentration values, and Obatã, a dwarf cultivar, the lowest values. Higher coffee yields and lower leaf P, Ca and S concentrations were observed in plots with one plant compared to the plots with two plants. In general, the coffee cultivars had leaf N and S concentrations above the reference limits reported in the literature, but leaf concentrations of other macronutrients were within adequate ranges.Cultivares de cafeeiro (Coffea Arabica L. adaptadas às regiões de cultivo, com população de plantas otimizada e adequado estado nutricional são premissas para a obtenção de produções elevadas de café. Estudou-se a produção trienal de café e o teor foliar de macronutrientes de cultivares de cafeeiro em função das densidades de plantio. Foram utilizados os cultivares Catuaí Amarelo (IAC 47, Obatã (IAC 1669-20, Acaiá (IAC 474-19 e Icatu Amarelo (IAC 2944 nas populações de 2.500 plantas ha-1 com duas plantas por cova; e, 5

  1. Farmers' experiences in the management of pests and diseases of ...

    African Journals Online (AJOL)

    Mo

    Evaluation of farmers' experiences on pests and diseases is important for the development and introduction of management ... involved in on-farm testing and promotion of calliandra in ... bananas (Musa species), coffee (Coffea arabica L.) and.

  2. Efeito da Xylella fastidiosa em cafeeiros em diferentes regiões edafoclimáticas Effect of Xylella fastidiosa in coffee plants at different edaphoclimatic regions

    Directory of Open Access Journals (Sweden)

    Rachel Benetti Queiroz-Voltan

    2005-01-01

    Full Text Available A bactéria Xylella fastidiosa vem causando problemas para a cafeicultura, uma vez que sua presença, associada a diversos fatores de estresse, provoca um decréscimo na produção devido à diminuição no número e tamanho dos frutos e à morte de alguns ramos. Este trabalho objetiva avaliar o efeito da X. fastidiosa sobre cultivares de Coffea arabica (enxertados ou não através da quantificação da proporção de vasos do xilema obstruídos pela bactéria, nas diferentes partes da planta e entre ramos com e sem sintoma da doença, em experimentos desenvolvidos em diferentes regiões edafoclimáticas. Avaliou-se também a distribuição das classes de infecção nas diferentes partes da planta nos materiais genéticos estudados. Os experimentos foram instalados em 1986 em Mococa e Garça (SP e as amostras para o estudo anatômico, retiradas em abril de 1998 e 2000 (período de estresse hídrico, respectivamente, das plantas de cafeeiros dessas áreas. Na região de Mococa, observou-se que a nervura principal e o pecíolo foram os tecidos com proporção maior de vasos do xilema obstruídos pela X. fastidiosa; na região de Garça, foram o pecíolo e o caule. Não houve diferenças significativas na obstrução de elementos de vaso do xilema do cafeeiro ocasionado pela bactéria entre as duas regiões estudadas. Não houve tolerância à bactéria nos materiais genéticos, havendo no entanto variação dentro de cada um deles. Na região de Garça, nas plantas de café, observou-se alta proporção de vasos obstruídos nas raízes (3%, entretanto, não houve dano maior na parte aérea.The Xylella fastidiosa bacterium causes problems to coffee cultivation because of a relationship with various stress factors, leading to reduction on coffee production by decreasing fruit number and size, as well as senescence of branches. This research aimed to evaluate the effect of Xylella fastidiosa to Coffea arabica cultivars (grafted or not through the

  3. Caracterização microclimática em cultivo consorciado café/banana Microclimatic characterization in coffee and banana intercrop

    Directory of Open Access Journals (Sweden)

    José R. M. Pezzopane

    2007-06-01

    Full Text Available Medições de radiação solar global, velocidade do vento, temperatura e umidade relativa do ar, foram realizadas em cafeeiros (Coffea arabica L. cv. Icatu Vermelho IAC 4045, cultivados a pleno sol e consorciados com bananeira (MusaspAAB 'Prata Anã', em Mococa, SP (21º 28' S, 47º 01' W, altitude 665 m, entre outubro de 2001 e setembro de 2002. Os elementos foram monitorados em um ponto do cultivo a pleno sol e em dois pontos do cultivo consorciado, sendo um próximo à planta de banana e outro situado em uma condição central, entre as plantas de banana. Os resultados obtidos mostraram que o cultivo consorciado de café promoveu atenuação dos valores médios da radiação solar global, tendo sido mais evidente no ponto amostral situado próximo às bananeiras. Verificou-se, ainda, uma redução média de 48% na velocidade do vento no cultivo consorciado, influenciada pela época do ano, além do desbaste das bananeiras. Com relação à temperatura e umidade do ar, foram encontradas diferenças apenas na temperatura máxima no ponto central da parcela do cultivo consorciado que, por sua vez, apresentou médias superiores em relação ao cultivo a pleno sol no verão e outono e em relação ao ponto situado próximo às bananeiras na primavera, verão e outono; essas diferenças foram mais significativas em dias com condição ensolarada e de pouca ocorrência de vento.Microclimatic measurements (solar radiation, wind speed, air temperature and relative humidity were taken on a daily and hourly basis, from October, 2001 to September, 2002 in a coffee crop (Coffea arabica L. cv. Icatu Verrmelho IAC 4045, grown in two different conditions: shaded by banana (Musa sp AAB 'Prata Anã' and unshaded. The experiment was carried out at Mococa, São Paulo State, Brazil (21º 28' S, 47º 01' W, altitude 665 m. The microclimatic measurements were taken in one position of the unshaded coffee crop and in two different positions of the shaded coffee crop

  4. Incidence of fruit flies on coffee and citrus and quarantine treatment of citrus fruits by gamma radiation

    International Nuclear Information System (INIS)

    Raga, Adalton

    1996-01-01

    The objective of this study was to evaluate the fruit fly infestation on coffee and citrus, and also to determine gamma radiation doses for immature stages of Ceratitis capitata and Anastrepha fraterculus, in order to satisfy quarantine regulations. Coffee arabica varieties Icatu Vermelho, Catuai Amarelo, Mundo Novo and Sarchimor showed the highest infestation indices (pupa/berry): 0.53; 0,41; 0.33 and 0.36. respectively Icatu Vermelho and Catuai Vermelho showed the highest values of pupa/berry weight (0.49 and 0.39, respectively), and Robusta (Coffea canephora) presented the lowest index (0.01). The following fruit flies were found in coffee berries: C. capitata (76.6%) Anastrepha spp. (7.4%) and Lonchaeidae (17.0%). In area near coffee plantation, fruit fly infestation indices in sweet oranges were of 4.77 larvae/kg and 0.55 larva/fruit. The infestation indices for sweet orange, collected from five regions of the State of Sao Paulo ranged from 0.73 to 7.60 pupa/kg and 0.12 to 1.27 pupa/fruit. The same species of fruit flies were found in oranges. In the case of C. capitata eggs with 24-48 hours old, 20 Gy prevented completely adult emergence (artificial diet and orange). No emergence of adult occurred when C. capitata larvae of third instar were irradiated at 20 Gy in their rearing medium. But at 25 Gy, the number of adults was reduced by 54% and 97% from larval infestation in oranges and grapefruit, respectively. A dose of 30 Gy was required to prevent medfly emergence from third instar larvae in grapefruit. A dose of 15 Gy was required for third instar, to prevent adult emergence of A. fraterculus. No adult emerged from C third instar, to prevent adult emergence of A. fraterculus. No adult emerged from C capitata pre-pupa irradiated at 30 Gy. One medfly adult emerged from pupa (3-4 days after pupating) irradiated at 120 Gy. At the same dose, sixteen A. fraterculus adults emergency from irradiated pupa with 5-6 days old. (author)

  5. Influence of coffee pruning on the severity of frost damage

    OpenAIRE

    Androcioli Filho,Armando; Caramori,Paulo Henrique

    2000-01-01

    Frost damages in a field experiment of pruning types and systems for the cultivars of Coffea arabica Catuaí and Mundo Novo, were evaluated at Londrina (23º22’S, 52º10´W), State of Parana, southern Brazil, during the winter of 1990 and 1994. Pruning types evaluated were ‘esqueletamento’ (cutting off all plagiotropic branches at 20-30 cm from the orthotropic branch), ‘decote’ (cutting off the orthotropic branch at 1.5 m and 2.0 m above ground) and ‘recepa’ (cutting off the orthotropic branch at...

  6. Coffee Production in Kavre and Lalitpur Districts, Nepal

    Directory of Open Access Journals (Sweden)

    Yogendra Kumar Karki

    2018-05-01

    Full Text Available Coffee (Coffea spp is an important and emerging cash crop having potential to provide farmers employment and income generation opportunities. This crop is well adapted to the climatic conditions of mid-hills of Nepal. Thus, majority of the farmers are attracted towards cultivation of coffee because of demands in national and international market. Coffee is now becoming integral part of farming system in rural areas. However, information on performance of coffee and farmers response has not been well documented. Therefore, we undertook the present work to analyze demography, ethnicity, household occupation, literacy status, average land holding, coffee cultivation area, livelihood and sources of income of coffee growers, production and productivity, pricing, cropping pattern of the coffee and problesm faced by them in mid hill district of Kavrepalanchowk (hereafter ‘Kavre’ and Lalitpur Districts. All the samples were taken randomly and selected from coffee producing cooperative of Kavre and Lalitpur. Our analysis showed that the male farmer dominant over female on adopting coffee cultivation in both districts with higher value in Kavre. Brahmin and Chetri ethnic communities were in majority over others in adopting the coffee cultivation. Literate farmers were more dominant over illiterates on adopting the coffee cultivation, The mean land holding was less, ranging from 0.15 to 2.30 ha for coffee cultivation, the history of coffee cultivation in Kavre showed that highest number of farmers were engaged in coffee farming from last 16 years. The mean yield of fresh cherry was 1027.20 kg/ha in Kavre, while it was 1849.36 kg/ha in Lalitpur. The study revealed that majority of the coffee plantations were between 6-10 years old. The major problems facing by coffee farmers were diseases spread, lack of irrigation facility and drying of plants. Despite of that the coffee farming was one of the rapidly emerging occupations among the farmers in both

  7. Microclimatic characterization and productivity of coffee plants grown under shade of pigeon pea in Southern Brazil Caracterização microclimática e produtividade de cafeeiros sombreados com guandu no Sul do Brasil

    Directory of Open Access Journals (Sweden)

    Heverly Morais

    2006-05-01

    Full Text Available Recent studies on coffee (Coffea arabica L. cultivation in agroforestry systems in Southern Brazil have shown the potential of partial shading to improve management of this crop. The objective of this work was to evaluate microclimatic conditions and their effects on coffee production of plants shaded with pigeon pea (Cajanus cajan in comparison to unshaded ones, from May 2001 to August 2002 in Londrina, State of Paraná, Brazil. The appraised microclimatic characteristics were: global radiation, photosynthetic and radiation balance; air, leaf and soil temperatures; and soil humidity. Shading caused significant reduction in incident global solar radiation, photosynthetically active radiation and net radiation, and attenuated maximum leaf, air and soil temperatures, during the day. Shade also reduced the rate of cooling of night air and leaf temperatures, especially during nights with radiative frost. Soil moisture at 0-10 cm depth was higher under shade. The shaded coffee plants produced larger cherries due to slower maturation, resulting in larger bean size. Nevertheless, plants under shade emitted less plagiotropic branches, with smaller number of nodes per branch, and fewer nodes with fruits, resulting in a large reduction in coffee production. These results show the need to find an optimal tree density and management that do not compromise coffee production and protect against extreme temperatures.Recentes estudos sobre cafeeiros (Coffea arabica L. cultivados em sistemas agroflorestais no Sul do Brasil têm mostrado o potencial do sombreamento parcial no manejo desta cultura. O objectivo deste trabalho foi avaliar as condições do microclima e seus efeitos na produção de café sombreado com guandu (Cajanus cajan, em comparação ao cultivado a pleno sol, no período de maio de 2001 a agosto de 2002 em Londrina, PR. As características microclimáticas avaliadas foram: radiação global, fotossintética e saldo de radiação; temperaturas

  8. Consequences of Stoichiometric Error on Nuclear DNA Content Evaluation in Coffea liberica var. dewevrei using DAPI and Propidium Iodide

    OpenAIRE

    NOIROT, MICHEL; BARRE, PHILIPPE; LOUARN, JACQUES; DUPERRAY, CHRISTOPHE; HAMON, SERGE

    2002-01-01

    The genome size of coffee trees (Coffea sp.) was assessed using flow cytometry. Nuclear DNA was stained with two dyes [4′,6‐diamino‐2‐phenylindole dihydrochloride hydrate (DAPI) and propidium iodide (PI)]. Fluorescence in coffee tree nuclei (C‐PI or C‐DAPI) was compared with that of the standard, petunia (P‐PI or P‐DAPI). If there is no stoichiometric error, then the ratio between fluorescence of the target nuclei and that of the standard nuclei (R‐PI or R‐DAPI) is expected to be proportional...

  9. Determination of the influence of the variation of reducing and non-reducing sugars on coffee quality with use of artificial neural network Determinação da influência da variação de açúcar redutor e não redutor na qualidade da bebida do café com utilização de rede neural artificial

    Directory of Open Access Journals (Sweden)

    José A. T. Messias

    2012-04-01

    Full Text Available The present study aimed at evaluating the use of Artificial Neural Network to correlate the values resulting from chemical analyses of samples of coffee with the values of their sensory analyses. The coffee samples used were from the Coffea arabica L., cultivars Acaiá do Cerrado, Topázio, Acaiá 474-19 and Bourbon, collected in the southern region of the state of Minas Gerais. The chemical analyses were carried out for reducing and non-reducing sugars. The quality of the beverage was evaluated by sensory analysis. The Artificial Neural Network method used values from chemical analyses as input variables and values from sensory analysis as output values. The multiple linear regression of sensory analysis values, according to the values from chemical analyses, presented a determination coefficient of 0.3106, while the Artificial Neural Network achieved a level of 80.00% of success in the classification of values from the sensory analysis.Este trabalho teve como objetivo avaliar a utilização de Rede Neural Artificial para correlacionar os valores resultantes de análises químicas de amostras de café com os valores de sua análise sensorial. As amostras de café utilizadas foram referentes ao café Coffea arabica L., cultivar Acaiá do Cerrado, Topázio, Acaiá 474-19 e Bourbon, coletados na região sul de Minas Gerais. As análises químicas foram de açúcar não redutor e açúcar redutor, sendo a qualidade da bebida avaliada pela análise sensorial. O método de Rede Neural Artificial utilizou os valores das análises químicas como variáveis de entrada e os valores da análise sensorial como valores de saída. A regressão linear múltipla dos valores de análise sensorial, em função dos valores das análises químicas, teve coeficiente de determinação de 0,3106, enquanto o método de Rede Neural Artificial empregado obteve um nível de acerto na classificação dos valores da análise sensorial de 80,00%.

  10. Frações oxidáveis do carbono orgânico de latossolo cultivado com cafeeiro em diferentes espaçamentos de plantio Oxidizible organic carbon fractions in a latosol cultivated with coffee at different planting spacings

    Directory of Open Access Journals (Sweden)

    Otacílio José Passos Rangel

    2008-04-01

    Full Text Available Objetivou-se com este estudo avaliar a suscetibilidade à oxidação pelo permanganato de potássio do carbono presente em Latossolo Vermelho distroférrico típico, cultivado com cafeeiro (Coffea arabica L., em diferentes espaçamentos de plantio, em experimento localizado na Fazenda Experimental da EPAMIG, em Machado (MG. Os tratamentos avaliados, instalados a campo em 1992, consistiram na combinação de quatro espaçamentos entre linhas (2,0, 2,5, 3,0 e 3,5 m, com três espaçamentos entre plantas (0,5, 0,75 e 1,0 m. Uma área de mata próxima ao experimento foi amostrada e usada como referência. Para a avaliação do grau de oxidação do carbono orgânico (CO, as amostras de solo foram coletadas na entrelinha (EL e na projeção da copa (PC do cafeeiro, nas camadas de solo de 0-0,05 e 0-0,1 m. Na camada superficial do solo (0-0,05 m, os teores de C lábil na entrelinha são, em geral, maiores do que os observados nas amostras coletadas na projeção da copa do cafeeiro. A amplitude de variação dos teores de C nas frações orgânicas obedece à seguinte ordem decrescente: C lábil> C não lábil > C orgânico, o que indica o maior potencial do C lábil em avaliar o impacto de diferentes sistemas de adensamento e manejo do cafeeiro, sobre os compartimentos de matéria orgânica. Os teores de C lábil e C não lábil não são influenciados pelo número de plantas por área, mas são dependentes da largura de rua do cafeeiro, da profundidade e do local de coleta das amostras de solo.The present work was carried out to evaluate soil carbon susceptibility to oxidation by potassium permanganate in adystroferric Red Latosol (Oxisol cultivated with coffee (Coffea arabica L. at different planting spacings. The experiment was installed in 1992 at the Experimental Farm of EPAMIG, in Machado (Minas Gerais State-Brazil. Treatments consisted of a combination of four row spacings (2.0, 2.5, 3.0, and 3.5 m with three spacings within the row (0.5, 0

  11. Spent coffee grounds as a versatile source of green energy.

    Science.gov (United States)

    Kondamudi, Narasimharao; Mohapatra, Susanta K; Misra, Mano

    2008-12-24

    The production of energy from renewable and waste materials is an attractive alternative to the conventional agricultural feed stocks such as corn and soybean. This paper describes an approach to extract oil from spent coffee grounds and to further transesterify the processed oil to convert it into biodiesel. This process yields 10-15% oil depending on the coffee species (Arabica or Robusta). The biodiesel derived from the coffee grounds (100% conversion of oil to biodiesel) was found to be stable for more than 1 month under ambient conditions. It is projected that 340 million gallons of biodiesel can be produced from the waste coffee grounds around the world. The coffee grounds after oil extraction are ideal materials for garden fertilizer, feedstock for ethanol, and as fuel pellets.

  12. Can Elevated Air [CO2] Conditions Mitigate the Predicted Warming Impact on the Quality of Coffee Bean?

    Science.gov (United States)

    Ramalho, José C.; Pais, Isabel P.; Leitão, António E.; Guerra, Mauro; Reboredo, Fernando H.; Máguas, Cristina M.; Carvalho, Maria L.; Scotti-Campos, Paula; Ribeiro-Barros, Ana I.; Lidon, Fernando J. C.; DaMatta, Fábio M.

    2018-01-01

    Climate changes, mostly related to high temperature, are predicted to have major negative impacts on coffee crop yield and bean quality. Recent studies revealed that elevated air [CO2] mitigates the impact of heat on leaf physiology. However, the extent of the interaction between elevated air [CO2] and heat on coffee bean quality was never addressed. In this study, the single and combined impacts of enhanced [CO2] and temperature in beans of Coffea arabica cv. Icatu were evaluated. Plants were grown at 380 or 700 μL CO2 L-1 air, and then submitted to a gradual temperature rise from 25°C up to 40°C during ca. 4 months. Fruits were harvested at 25°C, and in the ranges of 30–35 or 36–40°C, and bean physical and chemical attributes with potential implications on quality were then examined. These included: color, phenolic content, soluble solids, chlorogenic, caffeic and p-coumaric acids, caffeine, trigonelline, lipids, and minerals. Most of these parameters were mainly affected by temperature (although without a strong negative impact on bean quality), and only marginally, if at all, by elevated [CO2]. However, the [CO2] vs. temperature interaction strongly attenuated some of the negative impacts promoted by heat (e.g., total chlorogenic acids), thus maintaining the bean characteristics closer to those obtained under adequate temperature conditions (e.g., soluble solids, caffeic and p-coumaric acids, trigonelline, chroma, Hue angle, and color index), and increasing desirable features (acidity). Fatty acid and mineral pools remained quite stable, with only few modifications due to elevated air [CO2] (e.g., phosphorous) and/or heat. In conclusion, exposure to high temperature in the last stages of fruit maturation did not strongly depreciate bean quality, under the conditions of unrestricted water supply and moderate irradiance. Furthermore, the superimposition of elevated air [CO2] contributed to preserve bean quality by modifying and mitigating the heat impact

  13. Can Elevated Air [CO2] Conditions Mitigate the Predicted Warming Impact on the Quality of Coffee Bean?

    Directory of Open Access Journals (Sweden)

    José C. Ramalho

    2018-03-01

    Full Text Available Climate changes, mostly related to high temperature, are predicted to have major negative impacts on coffee crop yield and bean quality. Recent studies revealed that elevated air [CO2] mitigates the impact of heat on leaf physiology. However, the extent of the interaction between elevated air [CO2] and heat on coffee bean quality was never addressed. In this study, the single and combined impacts of enhanced [CO2] and temperature in beans of Coffea arabica cv. Icatu were evaluated. Plants were grown at 380 or 700 μL CO2 L-1 air, and then submitted to a gradual temperature rise from 25°C up to 40°C during ca. 4 months. Fruits were harvested at 25°C, and in the ranges of 30–35 or 36–40°C, and bean physical and chemical attributes with potential implications on quality were then examined. These included: color, phenolic content, soluble solids, chlorogenic, caffeic and p-coumaric acids, caffeine, trigonelline, lipids, and minerals. Most of these parameters were mainly affected by temperature (although without a strong negative impact on bean quality, and only marginally, if at all, by elevated [CO2]. However, the [CO2] vs. temperature interaction strongly attenuated some of the negative impacts promoted by heat (e.g., total chlorogenic acids, thus maintaining the bean characteristics closer to those obtained under adequate temperature conditions (e.g., soluble solids, caffeic and p-coumaric acids, trigonelline, chroma, Hue angle, and color index, and increasing desirable features (acidity. Fatty acid and mineral pools remained quite stable, with only few modifications due to elevated air [CO2] (e.g., phosphorous and/or heat. In conclusion, exposure to high temperature in the last stages of fruit maturation did not strongly depreciate bean quality, under the conditions of unrestricted water supply and moderate irradiance. Furthermore, the superimposition of elevated air [CO2] contributed to preserve bean quality by modifying and mitigating

  14. Can Elevated Air [CO2] Conditions Mitigate the Predicted Warming Impact on the Quality of Coffee Bean?

    Science.gov (United States)

    Ramalho, José C; Pais, Isabel P; Leitão, António E; Guerra, Mauro; Reboredo, Fernando H; Máguas, Cristina M; Carvalho, Maria L; Scotti-Campos, Paula; Ribeiro-Barros, Ana I; Lidon, Fernando J C; DaMatta, Fábio M

    2018-01-01

    Climate changes, mostly related to high temperature, are predicted to have major negative impacts on coffee crop yield and bean quality. Recent studies revealed that elevated air [CO 2 ] mitigates the impact of heat on leaf physiology. However, the extent of the interaction between elevated air [CO 2 ] and heat on coffee bean quality was never addressed. In this study, the single and combined impacts of enhanced [CO 2 ] and temperature in beans of Coffea arabica cv. Icatu were evaluated. Plants were grown at 380 or 700 μL CO 2 L -1 air, and then submitted to a gradual temperature rise from 25°C up to 40°C during ca. 4 months. Fruits were harvested at 25°C, and in the ranges of 30-35 or 36-40°C, and bean physical and chemical attributes with potential implications on quality were then examined. These included: color, phenolic content, soluble solids, chlorogenic, caffeic and p -coumaric acids, caffeine, trigonelline, lipids, and minerals. Most of these parameters were mainly affected by temperature (although without a strong negative impact on bean quality), and only marginally, if at all, by elevated [CO 2 ]. However, the [CO 2 ] vs. temperature interaction strongly attenuated some of the negative impacts promoted by heat (e.g., total chlorogenic acids), thus maintaining the bean characteristics closer to those obtained under adequate temperature conditions (e.g., soluble solids, caffeic and p -coumaric acids, trigonelline, chroma, Hue angle, and color index), and increasing desirable features (acidity). Fatty acid and mineral pools remained quite stable, with only few modifications due to elevated air [CO 2 ] (e.g., phosphorous) and/or heat. In conclusion, exposure to high temperature in the last stages of fruit maturation did not strongly depreciate bean quality, under the conditions of unrestricted water supply and moderate irradiance. Furthermore, the superimposition of elevated air [CO 2 ] contributed to preserve bean quality by modifying and mitigating

  15. Coffee vs. Cacao: A Case Study from the Vietnamese Central Highlands

    Science.gov (United States)

    Ha, Dang Thanh; Shively, Gerald

    2005-01-01

    Mr. Nam, the vice chair of a village in Dak Lak province of Vietnam, was keen to protect farmers in his village from the sharp decline in prices of coffee ("Coffea canephora" Pierre ex Froehner). He did this by encouraging farmers in his village to plant cacao ("Theobroma cacao" L. subsp. "cacao"). Cacao was suitable…

  16. Green Coffee

    Science.gov (United States)

    ... coffee product Coffee Slender (Med-Eq Ltd., Tonsberg, Norway), lose an average of 2.5 to 3. ... might increase the risk of experiencing serious or life-threatening side effects such as high blood pressure, ...

  17. Morphological Characterization and Identification of Coffea liberica Callus of Somatic Embryogenesis Propagation.

    Directory of Open Access Journals (Sweden)

    Fitria Ardiyani

    2015-08-01

    Full Text Available Compared with other types of coffee, Liberica coffee is more difficult to be propagates using clonal methods. Meanwhile, demand for planting materials and consumption of this type of coffee is increasing lately. The objective of this paper is to present results of the work on morpological characterization of Liberica coffee (Coffea liberica callus produced by somatic embryogenesis propagation. This research used C. liberica Arruminensis clone. This clone was one of Liberica coffee clones which had superior taste. Main activitis carried out in this experiment were explant sterilization, explant induction and histological analysis on the callus produced. The result of this research showed that non embryogenic callus was higher (72% than embryogenic callus (28%. The callus description can be used to identify type and characteristic of the callus. Therefore, it can be a parameter to choose type of callus for propagation material. This is important because choosing the right callus is determine of the succesfully process of Liberica somatic embryogenesis. Keywords: somatic embryogenesis, Liberica, embryogenic, non-embryogenic

  18. Prediction of specialty coffee cup quality based on near infrared spectra of green coffee beans.

    Science.gov (United States)

    Tolessa, Kassaye; Rademaker, Michael; De Baets, Bernard; Boeckx, Pascal

    2016-04-01

    The growing global demand for specialty coffee increases the need for improved coffee quality assessment methods. Green bean coffee quality analysis is usually carried out by physical (e.g. black beans, immature beans) and cup quality (e.g. acidity, flavour) evaluation. However, these evaluation methods are subjective, costly, time consuming, require sample preparation and may end up in poor grading systems. This calls for the development of a rapid, low-cost, reliable and reproducible analytical method to evaluate coffee quality attributes and eventually chemical compounds of interest (e.g. chlorogenic acid) in coffee beans. The aim of this study was to develop a model able to predict coffee cup quality based on NIR spectra of green coffee beans. NIR spectra of 86 samples of green Arabica beans of varying quality were analysed. Partial least squares (PLS) regression method was used to develop a model correlating spectral data to cupping score data (cup quality). The selected PLS model had a good predictive power for total specialty cup quality and its individual quality attributes (overall cup preference, acidity, body and aftertaste) showing a high correlation coefficient with r-values of 90, 90,78, 72 and 72, respectively, between measured and predicted cupping scores for 20 out of 86 samples. The corresponding root mean square error of prediction (RMSEP) was 1.04, 0.22, 0.27, 0.24 and 0.27 for total specialty cup quality, overall cup preference, acidity, body and aftertaste, respectively. The results obtained suggest that NIR spectra of green coffee beans are a promising tool for fast and accurate prediction of coffee quality and for classifying green coffee beans into different specialty grades. However, the model should be further tested for coffee samples from different regions in Ethiopia and test if one generic or region-specific model should be developed. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. Characterisation of AC1: a naturally decaffeinated coffee

    Directory of Open Access Journals (Sweden)

    Luciana Benjamim Benatti

    2012-01-01

    Full Text Available We compared the biochemical characteristics of the beans of a naturally decaffeinated Arabica coffee (AC1 discovered in 2004 with those of the widely grown Brazilian Arabica cultivar "Mundo Novo" (MN. Although we observed differences during fruit development, the contents of amino acids, organic acids, chlorogenic acids, soluble sugars and trigonelline were similar in the ripe fruits of AC1 and MN. AC1 beans accumulated theobromine, and caffeine was almost entirely absent. Tests on the supply of [2-14C] adenine and enzymatic analysis of theobromine synthase and caffeine synthase in the endosperm of AC1 confirmed that, as in the leaves, caffeine synthesis is blocked during the methylation of theobromine to caffeine. The quality of the final coffee beverage obtained from AC1 was similar to that of MN.

  20. Redução de nitrato em plantas jovens de café cultivadas em diferentes níveis de luz e de nitrogênio Nitrate reduction in young coffee trees grown under different levels of light and nitrogen

    Directory of Open Access Journals (Sweden)

    Maria Luiza Carvalho Carelli

    1990-01-01

    Full Text Available Foi estudado o efeito de níveis de luz e de nitrogênio na atividade da enzima redutase de nitrato e nos teores de nitrato e de açúcares nas folhas de plantas jovens de café (Coffea arabica L, assim como as possíveis relações entre a disponibilidade desses compostos e a atividade enzimática. Foram utilizadas plantas de dez meses de idade cultivadas em vasos contendo uma mistura de terra mais composto, e mantidas em condições ambientais em pleno sol e em 50% da luz solar. Metade das plantas de cada tratamento de luz foi suplementada semanalmente com nitrogênio. Os resultados mostraram que a atividade da redutase de nitrato, nos dois tratamentos de luz, foi maior nas plantas suplementadas com nitrogênio. Para um mesmo nível de nitrogênio, as plantas cultivadas em pleno sol apresentaram menor atividade da redutase de nitrato, maiores teores de nitrato e de açúcares e maiores taxas de transpiração, do que as cultivadas na sombra. Tais resultados indicam que a menor atividade da redutase de nitrato nas plantas cultivadas em pleno sol aparentemente não foi devida a limitações na disponibilidade de nitrato e de açúcares para fornecer a energia necessária para a redução de nitrato.The effect of levels of light and nitrogen on the activity of the enzyme nitrate reductase and its relationship with the availability of sugars and nitrate was studied in leaves of coffee plants (Coffea arabica L. cv. Catual. Ten month old plants were grown on pots containing a mixture of soil and compost, and were kept at full or 50% sunlight. Half of the plants of each light treatment received nitrogen supply. The results showed that the activity of nitrate reductase was higher on plants supplied with nitrogen at both light treatments. For the same nitrogen level, plants grown under full sunlight presented lower nitrate reductase activity, higher nitrate and sugars concentrations, and higher transpiration rates than plants kept at 50% sunlight

  1. Plant regeneration through somatic embryogenesis in three ethiopian Coffea arabica Lin. hybrids

    Directory of Open Access Journals (Sweden)

    RA Ramos

    2009-01-01

    Abbreviations: BA (6-benzyladenine; 2,4-D (2,4-dichlorophenoxyacetic acid; IBA (indole-3-butyric acid; MS (Murashige and Skoog; NAA (naphthaleneacetic acid, GA3 (Gibberellic acid, CV (Coefficient of variation, S.E (Standard error.

  2. Melhoramento do cafeeiro: XIX - Pesquisas sôbre o café semperflorens Coffee breeding: XIX - Researches on the semperflorens coffee

    Directory of Open Access Journals (Sweden)

    Célio S. Novaes Antunes

    1960-01-01

    -se, também, a tendência de crescimento dos valores da peneira média, a partir de janeiro, cujo máximo ocorreu em junho, declinando até dezembro. Tem-se que tal variabilidade mensal está ligada a épocas diferentes de desenvolvimento dos frutos. O pêso médio de 100 sementes não se mostrou muito variável, é da ordem de 10,52 gramas, exceto em 1941, em que se revelou menor e correspondeu à menor produção anual e aos valores mais reduzidos de peneira méida. Nos meses, os maiores valores se verificaram a partir de abri], com máximo em julho. Não se mostraram correlacionados os dados médios mensais do pêso de sementes e as produções médias mensais; obteve-se, entretanto, correlação positiva entre o pêso de sementes e os valores mensais de peneira média. As quantidades de sementes do tipo moca revelaram-se bem variáveis. Assinalaram-se, em seis anos de determinações, limites extremos de 3,5% e 23,0%. Nos meses, as porcentagens mais elevadas desse tipo de semente ocorreram em junho e julho, quando a produção é reduzida; os valores mínimos se situaram no período de outubro a dezembro. Dada a produtividade não elevada do café semperflorens, o cultivo em larga escala dêsae cafeeiro deixou de apresentar interesse econômico. Contudo, êsse mutante, pelo contínuo florescimento que exibe, poderá desempenhar um valor todo especial para as análises genéticas do café e, também, para as pesquisas fisiológicas.The main feature of the semperflorens mutant of Coffea arabica is its ability to produce flowers during the whole year, whereas other commercial types of the same species have a definitive flowering period. In the S. Paulo plateau this occurs only at the beginning of the rainy season, August-October. The yield of several progenies of the semperflorens coffee, the period of fruit development, and the size and types of seeds produced were recorded for a few years in plantings made in three localities of the state of S. Paulo: Campinas (1940

  3. Sensitive response and resistance to bery disease ( Colletotrichum ...

    African Journals Online (AJOL)

    Seedling hypocotyls and attached green coffee berries of 11 Coffea arabica varieties and a Robusta coffee cultivar, with different levels of resistance to coffee berry disease (Colletotrichum kahawae), were examined under a microscope for differences in the development of infections caused by single-conidium isolates of ...

  4. Seed bank from soil of coffee plantations associated with grevillea trees Banco de sementes do solo proveniente de cafezais arborizados com grevílea

    Directory of Open Access Journals (Sweden)

    M.A.F. Santos

    2008-01-01

    Full Text Available This study aimed to study the composition and dynamics of seed bank from soil of coffee plantations associated with grevilea trees in the experimental fields of the Southwest Bahia State University, on Vitória da Conquista campus. The experiments were carried out from September 2006 to May 2007. The coffee trees (Coffea arabica were sown at three x one m spacing, associated with grevillea trees (Grevillea robusta and maintained at densities of 277, 139, 123, 69, 62 and 31 plants ha-1, under direct sunlight. One hundred grams of soil were taken from each treatment with four repetitions and later identified and counted with a 10x magnifying glass. To determine seedling emergence, four soil samples of 1000 g were collected from each experimental field and transported to the greenhouse. Seedling emergence was observed by counts after 15, 30 and 45 days. The experimental design was randomized blocks of seven treatments (soil from different tree densities and four replicates; the experimental unit consisted of a plastic tray (0, 30 x 0.22 x 0.06 m containing 1.000 g of soil. The variables utilized to characterize the bank and its dynamics were: relative frequency, relative density, relative abundance, importance index and species diversity (Shannon-Weaver index.Increased number of monocotyledon seeds and sprouts were verified in the treatments maintained under full sunlight.Com o objetivo de estudar a composição e a dinâmica do banco de sementes provenientes de solos de cafezais arborizados com grevíleas, desenvolveu-se um ensaio no campo experimental da Universidade Estadual do Sudoeste da Bahia, no campus de Vitória da Conquista. Os cafeeiros (Coffea arabica foram dispostos em espaçamento de 3 x 1 m, associados a grevíleas (Grevillea robusta mantidas em densidades de 277, 139, 123, 69, 62 e 31 plantas ha-1, e a pleno sol, no período de setembro de 2006 a maio de 2007. Para avaliação do número de sementes, foram retirados 100 g de solo de

  5. Invertase and sucrose synthase activities in coffee plants sprayed with sucrose solution Atividade de invertases e sacarose sintase em plantas de cafeeiro pulverizadas com solução de sacarose

    Directory of Open Access Journals (Sweden)

    José Carlos da Silva

    2003-01-01

    Full Text Available One management practice of which the efficiency has not yet been scientifically tested is spraying coffee plants with diluted sucrose solutions as a source of carbon for the plant. This paper evaluates the effect of foliar spraying with sugar on the endogenous level of carbohydrates and on the activities of invertase and sucrose synthase in coffee (Coffea arabica L. seedlings with reduced (low and high (normal levels of carbon reserve. The concentrations used were 0.5 and 1.0% sucrose, and water as a control. The use of sucrose at 1.0% caused an increase in the concentration of total soluble sugars in depauperate plants, as well as increased the activity of the following enzymes: cell wall and vacuole acid invertase, neutral cytosol invertase and sucrose synthase. In plants with high level of carbon reserve, no increments in total soluble sugar levels or in enzymatic activity were observed. Regardless of treatments or plants physiological state, no differences in transpiration or stomatal conductance were observed, demonstrating the stomatal control of transpiration. Photosynthesis was stimulated with the use of 0.5 and 1.0 % sucrose only in depauperate plants. Coffee seedling spraying with sucrose is only efficient for depauperate plants, at the concentration of 1.0%.Uma prática cuja eficiência não foi ainda comprovada cientificamente, é a pulverização dos cafeeiros com solução diluída de sacarose, como fonte de carbono para as plantas. Este trabalho visou estudar o efeito da pulverização de açúcar via folha nos teores endógenos de carboidratos e na atividade das enzimas invertases e sacarose sintase em mudas de cafeeiros (Coffea arabica L. com baixo (baixo e alto (normal nível de reservas de carbono. As pulverizações ocorreram nas concentrações de 0,5 e 1% de sacarose utilizando-se água como testemunha. A aplicação de sacarose a 1% aumentou a concentração de açúcares solúveis totais (AST em plantas depauperadas, como

  6. Qualidade de grãos de café beneficiados em resposta à adubação potássica Potassium fertilization and the quality of processed coffee beans

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    Enilson de Barros Silva

    2002-03-01

    Full Text Available O clima e o solo tem elevada influência na qualidade dos grãos de café (Coffea arabica L. beneficiado. Foram instalados dois experimentos sobre latossolo (Latossolo Vermelho distroférrico e Latossolo Vermelho-Amarelo distrófico com o objetivo de verificar a qualidade dos grãos de café beneficiados submetidos à adubação potássica em duas condições edafoclimáticas. Em ambos os locais, os experimentos foram delineados em blocos casualizados, em esquema de parcelas subdivididas, utilizando-se três fontes de K: cloreto de potássio (KCl, sulfato de potássio (K2SO4 e nitrato de potássio (KNO3 nas parcelas e quatro doses de K (0, 100, 200 e 400 kg ha-1 aplicadas nas subparcelas com quatro repetições. Usou-se nos experimentos o cultivar Catuaí Vermelho no espaçamento 3,5 x 0,7 m, com uma planta por cova. As avaliações foram: atividade enzimática da polifenoloxidase, índice de coloração e açúcares totais. Os valores das características qualitativas dos grãos mostraram que a fonte KCl teve uma resposta inferior em termos de qualidade dos grãos em relação às fontes K2SO4 e KNO3. Estas últimas fontes tiveram melhor resposta quando aplicadas nas condições de São Sebastião do Paraíso do que nas de Patrocínio. Em termos de doses aplicadas, os melhores resultados para qualidade dos grãos foram obtidos com as doses de 200 kg de K ha-1 na forma de KCl e K2SO4 e 100 kg de K ha-1 na forma de KNO3.Climate and soil strongly influence the quality of processed coffee (Coffea arabica L. beans. This work studied the influence of potassium fertilization on the quality of processed coffee beans grown on two Oxisols (Rhodic Acrudox and Xanthic Acrustox. Trials were set up in a completely randomized split plot block design, to test the influence of three sources and four potassium rates - potassium chloride (KCl, potassium sulphate (K2SO4 and potassium nitrate (KNO3 at 0; 100; 200 and 400 kg ha-1, applied to plants of cv. Catua

  7. Resistência parcial à necrose dos frutos em genótipos de café arábica Partial resistance to fruit necrosis in arabic coffee genotypes

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    Gustavo Hiroshi Sera

    2009-01-01

    Full Text Available Neste trabalho, objetivou-se identificar em genótipos de café arábica (Coffea arabica L. fontes de resistência à necrose de frutos em condições de campo. As variáveis resistência à necrose de frutos ("NF", quantidade média de frutos por nó produtivo ("FN" e índice de luminosidade dentro da copa da planta ("LUM" foram avaliadas em ensaio de campo no IAPAR (Londrina, em dezembro de 2004, em cafeeiros com três anos de idade. Foi avaliada a variável "NF", atribuindo notas de 1 a 5 sendo 1 para plantas com 0 % a 5 % de frutos necrosados. "FN" foi avaliada seguindo uma escala de notas de 1 a 5, onde 1 representa plantas com 0 a 3 frutos/ nó e nota 5 com mais do que 15 frutos/ nó. A avaliação da variável "LUM" foi visual e seguiu as notas de 1 a 5, onde 1 representa plantas com pouca luminosidade dentro da copa da planta. Dos 19 genótipos avaliados, 2 apresentaram moderada resistência, 7 moderada suscetibilidade, 5 suscetíveis e 5 altamente suscetíveis. As cultivares Obatã IAC 1669-20 e Catucaí Vermelho 4-79 apresentaram o melhor nível de resistência parcial de campo, ou seja, com moderada resistência à necrose de frutos associada ao Colletotrichum spp.The aim of this research was to identify in Coffea arabica L. coffee genotypes resistance sources to fruit necrosis in field conditions. The variables fruit necrosis ("NF", fruits per productive nodes average ("FN" and luminosity index inside of the plant canopy ("LUM" were evaluated in field assay at IAPAR (Londrina, in December 2004 on three years old coffee trees. The "NF" variable were evaluated attributing scores from 1 to 5, where score 1 represented plants with 0 to 5 % of necrosed fruits. "FN" were evaluated following a score scale from 1 to 5, where score 1 represented plants with 0 to 3 fruits/ node and score 5 with more than 15 fruits/ node. The evaluation of the variable "LUM" was visual and followed the scores from 1 to 5, where score 1 represented plants with

  8. Efeito da exclusão dos insetos polinizadores na produção do café Bourbon The effect of the exclusion of pollinating insects on the yield of the bourbon coffee

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    Paulo Nogueira - Neto

    1959-01-01

    oriundos de Coffea Dewevrei, que são maiores do que as do café Bourbon e têm perfume mais intenso. Apesar da ocorrência dos insetos colhendo néctar e pólen, os dados de produção de café cereja não mostraram diferenças significativas entre as plantas protegidas e sem proteção. Notou-se apenas uma tendência, em cinco dos seis anos analisados, de serem maiores as produções das plantas sem proteção e, portanto, visitadas por insetos. Os dados de frutificação obtidos indicaram, também, melhor pegamento dos frutos nas plantas sem proteção. Nestes cafeeiros as porcentagens de sementes moca mostraram-se significativamente maiores, enquanto as porcentagens de sementes concha, embora também maiores, não se mostraram significativas. A quantidade de frutos com lojas sem sementes e o tamanho das sementes não diferiram nos dois tratamentos. A influência das abelhas na polinização do cafeeiro deve, pois, ser pràticamente limitada às espécies autoestéreis de Coffea. Os dados aqui obtidos indicam que o papel desempenhado pelos insetos em promover maior polinização e aumento de produção dos cafeeiros da var. bourbon, é de importância secundária.The diploid species of Coffea so far investigated, are self sterile, while tetraploid C. arabica, besides being self-fertile, is an almost completely autogamous species. Natural crossing in this species is promoted either by wind or by insects while gravity probably only plays a limited role. The trial here discussed was established to study the effect of certain native and also of the European honey bee in the promotion of the pollination in C. arabica var. bourbon, and also to test their influence on the yield of coffee. As it was difficult to specifically determine the effects of only this group of insects, the data represent the total effect on coffee yield and seed characteristics, of the exclusion of all pollinating insects. A group of 135 coffee "hills", each with four seedlings, and planted in 15 rows

  9. Coffee intake.

    Science.gov (United States)

    Cornelis, Marilyn C

    2012-01-01

    Coffee is one of the most widely consumed beverages in the world. Its widespread popularity and availability has fostered public health concerns of the potential health consequences of regular coffee consumption. Epidemiological studies of coffee intake and certain health outcomes have been inconsistent. The precise component of coffee potentially contributing to development of these conditions also remains unclear. One step toward addressing the challenges in studying the impact coffee has on health is a better understanding of the factors contributing to its consumption and physiological effects. This chapter focuses on those factors that are genetically determined and briefly summarizes progress in applying this knowledge to epidemiological studies of coffee and disease. Copyright © 2012 Elsevier Inc. All rights reserved.

  10. Compostos não voláteis em cafés da região sul de minas submetidos a diferentes pontos de torração Non-volatile compounds in coffee from the south of Minas Gerais state region submitted to different roasting degrees

    Directory of Open Access Journals (Sweden)

    Mirian Pereira Rodarte

    2009-10-01

    Full Text Available O processo de torração do café induz alterações físicas, químicas e sensoriais na matéria-prima, cuja intensidade e tipo dependem, principalmente, da composição química dos grãos e do tempo e temperatura utilizados na execução do mesmo. Este processo promove a degradação, formação e volatilização de vários compostos. Comercialmente, existem cafés torrados e moídos em diferentes pontos de torração, os quais originam bebidas com diferentes propriedades sensoriais e nutricionais. Os compostos trigonelina, ácidos clorogênicos e cafeína interferem no sabor e aroma do café. A trigonelina e os ácidos clorogênicos vêm sendo estudados também quanto ao aspecto nutricional, uma vez que esses compostos possuem ação benéfica à saúde. Esses compostos são solúveis em água quente, portanto, estarão presentes na bebida em função da sua estabilidade no processo de torração. Conduziu-se este trabalho, com o objetivo de avaliar as concentrações dos compostos trigonelina, ácidos clorogênicos e cafeína em cafés da espécie Coffea arabica L. classificados como bebida mole, dura e rio submetidos a três pontos de torração: claro, médio e escuro. As torrações mais acentuadas promoveram uma maior degradação de trigonelina e ácido 5-cafeoilquínico, enquanto que a torração clara promoveu degradação apenas para o ácido clorogênico, não interferindo nas concentrações de trigonelina. A degradação da cafeína não ocorreu em nenhum ponto de torração.The roasting process of coffee induces sensorial, chemical and physical alterations in the raw material. The type and intensity of the process depend mainly on the chemical composition of the grains and on the time and temperature used. This process promotes the degradation, formation and volatilisation of several compounds. There are roast and ground coffees submitted to different roasting conditions available in the market, which give origin to beverages

  11. Climate change impacts on coffee rust disease

    Science.gov (United States)

    Alfonsi, W. M. V.; Koga-Vicente, A.; Pinto, H. S.; Alfonsi, E. L., Sr.; Coltri, P. P.; Zullo, J., Jr.; Patricio, F. R.; Avila, A. M. H. D.; Gonçalves, R. R. D. V.

    2016-12-01

    Changes in climate conditions and in extreme weather events may affect the food security due to impacts in agricultural production. Despite several researches have been assessed the impacts of extremes in yield crops in climate change scenarios, there is the need to consider the effects in pests and diseases which increase losses in the sector. Coffee Arabica is an important commodity in world and plays a key role in Brazilian agricultural exports. Although the coffee crop has a world highlight, its yield is affected by several factors abiotic or biotic. The weather as well pests and diseases directly influence the development and coffee crop yield. These problems may cause serious damage with significant economic impacts. The coffee rust, caused by the fungus Hemileia vastarix,is among the diseases of greatest impact for the crop. The disease emerged in Brazil in the 70s and is widely spread in all producing regions of coffee in Brazil, and in the world. Regions with favorable weather conditions for the pathogen may exhibit losses ranging from 30% to 50% of the total grain production. The evaluation of extreme weather events of coffee rust disease in futures scenarios was carried out using the climatic data from CMIP5 models, data field of coffee rust disease incidence and, incubation period simulation data for Brazilian municipalities. Two Regional Climate Models were selected, Eta-HadGEM2-ES and Eta-MIROC5, and the Representative Concentration Pathways 8.5 w/m2 was adopted. The outcomes pointed out that in these scenarios the period of incubation tends to decrease affecting the coffee rust disease incidence, which tends to increase. Nevertheless, the changing in average trends tends to benefit the reproduction of the pathogen. Once the temperature threshold for the disease reaches the adverse conditions it may be unfavorable for the incidence.

  12. Incidence of fruit flies on coffee and citrus and quarantine treatment of citrus fruits by gamma radiation; Incidencia de moscas-das-frutas em cafe e citros e tratamento quarentenario de frutos citricos com radiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Raga, Adalton

    1997-12-31

    The objective of this study was to evaluate the fruit fly infestation on coffee and citrus, and also to determine gamma radiation doses for immature stages of Ceratitis capitata and Anastrepha fraterculus, in order to satisfy quarantine regulations. Coffee arabica varieties Icatu Vermelho, Catuai Amarelo, Mundo Novo and Sarchimor showed the highest infestation indices (pupa/berry): 0.53; 0,41; 0.33 and 0.36. respectively Icatu Vermelho and Catuai Vermelho showed the highest values of pupa/berry weight (0.49 and 0.39, respectively), and Robusta (Coffea canephora) presented the lowest index (0.01). The following fruit flies were found in coffee berries: C. capitata (76.6%) Anastrepha spp. (7.4%) and Lonchaeidae (17.0%). In area near coffee plantation, fruit fly infestation indices in sweet oranges were of 4.77 larvae/kg and 0.55 larva/fruit. The infestation indices for sweet orange, collected from five regions of the State of Sao Paulo ranged from 0.73 to 7.60 pupa/kg and 0.12 to 1.27 pupa/fruit. The same species of fruit flies were found in oranges. In the case of C. capitata eggs with 24-48 hours old, 20 Gy prevented completely adult emergence (artificial diet and orange). No emergence of adult occurred when C. capitata larvae of third instar were irradiated at 20 Gy in their rearing medium. But at 25 Gy, the number of adults was reduced by 54% and 97% from larval infestation in oranges and grapefruit, respectively. A dose of 30 Gy was required to prevent medfly emergence from third instar larvae in grapefruit. A dose of 15 Gy was required for third instar, to prevent adult emergence of A. fraterculus. No adult emerged from C third instar, to prevent adult emergence of A. fraterculus. No adult emerged from C capitata pre-pupa irradiated at 30 Gy. One medfly adult emerged from pupa (3-4 days after pupating) irradiated at 120 Gy. At the same dose, sixteen A. fraterculus adults emergency from irradiated pupa with 5-6 days old. (author) 85 refs., 2 figs., 13 tabs.

  13. Incidence of fruit flies on coffee and citrus and quarantine treatment of citrus fruits by gamma radiation; Incidencia de moscas-das-frutas em cafe e citros e tratamento quarentenario de frutos citricos com radiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Raga, Adalton

    1996-12-31

    The objective of this study was to evaluate the fruit fly infestation on coffee and citrus, and also to determine gamma radiation doses for immature stages of Ceratitis capitata and Anastrepha fraterculus, in order to satisfy quarantine regulations. Coffee arabica varieties Icatu Vermelho, Catuai Amarelo, Mundo Novo and Sarchimor showed the highest infestation indices (pupa/berry): 0.53; 0,41; 0.33 and 0.36. respectively Icatu Vermelho and Catuai Vermelho showed the highest values of pupa/berry weight (0.49 and 0.39, respectively), and Robusta (Coffea canephora) presented the lowest index (0.01). The following fruit flies were found in coffee berries: C. capitata (76.6%) Anastrepha spp. (7.4%) and Lonchaeidae (17.0%). In area near coffee plantation, fruit fly infestation indices in sweet oranges were of 4.77 larvae/kg and 0.55 larva/fruit. The infestation indices for sweet orange, collected from five regions of the State of Sao Paulo ranged from 0.73 to 7.60 pupa/kg and 0.12 to 1.27 pupa/fruit. The same species of fruit flies were found in oranges. In the case of C. capitata eggs with 24-48 hours old, 20 Gy prevented completely adult emergence (artificial diet and orange). No emergence of adult occurred when C. capitata larvae of third instar were irradiated at 20 Gy in their rearing medium. But at 25 Gy, the number of adults was reduced by 54% and 97% from larval infestation in oranges and grapefruit, respectively. A dose of 30 Gy was required to prevent medfly emergence from third instar larvae in grapefruit. A dose of 15 Gy was required for third instar, to prevent adult emergence of A. fraterculus. No adult emerged from C third instar, to prevent adult emergence of A. fraterculus. No adult emerged from C capitata pre-pupa irradiated at 30 Gy. One medfly adult emerged from pupa (3-4 days after pupating) irradiated at 120 Gy. At the same dose, sixteen A. fraterculus adults emergency from irradiated pupa with 5-6 days old. (author) 85 refs., 2 figs., 13 tabs.

  14. Populações de minhocas em sistemas agroflorestais com café convencional e orgânico Earthworms populations in agroforestry systems with conventional and organic coffee

    Directory of Open Access Journals (Sweden)

    Adriana Maria de Aquino

    2008-08-01

    Full Text Available Objetivou-se, neste estudo, determinar se as populações das minhocas são alteradas em função do manejo do café (Coffea arabica em Turrialba, CostaRica. Os seguintes sistemas de cultivo do café foram estudados: a pleno sol (PS e sombreado com eritrina, Erythrina poeppigiana (E; terminalia, Terminalia amazonia (T e cashá, Chloroleucon eurycyclum (Ab. A hipótese foi de que o manejo orgânico do café e o fornecimento da serapilheira de melhor qualidade favoreceria a diversidade, a densidade e a biomassa das minhocas. As populações das minhocas foram alteradas, em função do manejo com insumos sintéticos ou orgânicos , sendo a densidade menor no café a pleno sol. Entre as espécies utilizadas no sombreamento, a eritrina parece limitar a abundância das minhocas. Contudo, favorece a diversidade das mesmas, tendo sido registradas duas espécies de minhocas com papéis ecológicos diferenciados, Pontoscolex corethrurus, endogeica e Metaphire californica, anécica; ao contrário dos demais tratamentos, onde somente foi encontrada a primeira espécie, considerada cosmopolita com distribuição pantropical.The aim of this study was to determine whether the populations of the earthworms are altered by coffee systems in Turrialba, Costa Rica. The following coffee management systems were studied: the sun and shading with Erythrina poeppigiana; terminalia, Terminalia amazonia or cashá, Chloroleucon eurycyclum. The hypothesis was that the organic management of the coffee and the litter input of better quality would favor the diversity, the density and the biomass of the earthworms. The populations of earthworms were differentiated with the synthetic or organic input. However, the density was lower in the coffee under the sun anyone the used species in the agroforestry, the eritrina seems to limit the abundance of the earthworms, but it favors the diversity of the same ones, being registered two species of earthworms with differentiated

  15. Fotossíntese, relações hídricas e crescimento de cafeeiros jovens em relação à disponibilidade de fósforo Photosynthesis, water relations and growth of young coffee plants according to phosphorus availability

    Directory of Open Access Journals (Sweden)

    Leandro da Silva

    2010-09-01

    Full Text Available O objetivo deste trabalho foi avaliar de que maneira a alta disponibilidade de fósforo no solo afeta a fotossíntese e o crescimento de mudas de cafeeiro arábica (Coffea arabica. Mudas da cultivar Ouro Verde com aproximadamente quatro meses de idade, cultivadas com boa disponibilidade hídrica, foram submetidas a três tratamentos quanto à disponibilidade de fósforo: quantidade recomendada de P, na literatura (PA; duas vezes a dosagem utilizada em PA (P+; e sem adição de P ao solo (P-. Após 70 dias da aplicação dos tratamentos, foram avaliados: as trocas gasosas, a atividade fotoquímica, o potencial de água da folha, a condutância hidráulica da planta (K L, a partição de matéria seca na planta, os teores de pigmentos e carboidratos, e a composição química das folhas. O tratamento P- influenciou negativamente a fotossíntese, e levou à restrição do crescimento das plantas. As plantas do tratamento P+ apresentaram maior teor foliar de P (~1,9 g kg-1, com incrementos na assimilação de CO2, na eficiência instantânea de carboxilação e na atividade fotoquímica - maior eficiência do fotossistema II e maior transporte aparente de elétrons - em relação às plantas do tratamento PA. Houve aumento em K L, maior teor de carboidratos foliares e maior teor de clorofila nas plantas que receberam o dobro da dose recomendada de P, as quais apresentaram maior produção de matéria seca em relação às de PA e P-.The objective of this work was to evaluate how high soil phosphorus (P availability affects the photosynthesis and growth of young coffee arabica plants (Coffea arabica. Four months old coffee seedlings of the Ouro Verde cultivar, cultivated in good hydric conditions, were exposed to three P availability treatments: P dosage recommended in the literature (RP; two times the recommended dosage (P+; and without P (P-. Seventy days after treatment application, evaluations of leaf gas exchange, photochemical activity, leaf

  16. Brazilian Coffee Production as Function of Global Warming.

    Science.gov (United States)

    Avila, A. M. H. D.; Pinto, H. S.; Alfonsi, E. L., Sr.; Alfonsi, W. M. V.; Pereira, V. R.

    2016-12-01

    According to the Brazilian Government the actual area of coffee production in the country is close to 2.25 million hectares. The sector involves 290.000 of farmers with a production of 44 million of 60 Kg bags in 2015. The Arabica Coffee specie is cultivated in the country where the climate condition are characterized by a year mean temperatures between 18°C and 22°C. Temperatures higher than 33°C can cause abortion of flowers during the spring season and reduce the production while lower than 18°C can be affected by frost during winter when the minimum temperature can be lower than 2°C in the shelter. For a better quality of the final product the winter, between July and August, must be dry with rainfall lower than 50 mm/month. The Ministry of Agriculture defines those conditions for the Official Coffee Climatic Risk Zoning. In 2002, a partnership with the British Embassy and 2 Brazilian institutions, i. e. the State University of Campinas - UNICAMP and the Brazilian Agricultural Research Corporation - Embrapa, published the study "Global Warming and the New Geography of Agricultural Production in Brazil" (Pinto and Assad, 2002). This study was based on the PRECIS/Hadley Centre Regional Climate Model future projections. The crop simulations indicated a decrease in the grain production due to temperature rise. Later in 2012, a new study was developed in cooperation with the World Bank to evaluate the future of nine main commodities in Brazil under climate change, including the Arabica coffee. The worst scenario considering any mitigation and adaptation action indicated that the two most affected crops would be the soybean and coffee, with a reduction of 22% and 6.7 % in the yield respectively. Field surveys to evaluate the historical spatial dynamic and migration of Arabica coffee cultivated areas confirmed the results of the previous studies and indicated a recent increase in the search for cooler altitude areas to plant coffee. Also the field observations

  17. Activity of some isoenzymatic systems in stored coffee grains

    Directory of Open Access Journals (Sweden)

    Reni Saath

    2014-02-01

    Full Text Available Considering the worldwide consumption of coffee, it is natural that throughout the history many people have dedicated the research to markers that contribute somehow on gauging its quality. This research aimed to evaluate the biochemical performance of arabica coffee during storage. Coffee in beans (natural and in parchment (pulped dried in concrete terrace and in dryer with heated air were packed in jute bags and stored in not controlled environmental conditions. Enzymatic activities of superoxide dismutase, catalase, peroxidase, polyphenoloxidase, esterase and lipoxygenase in coffee grains were evaluated at zero, three, six, nine and twelve months by means of electrophoresis. Independently of the drying method, the activity of isoenzymatic complexes highlighted deteriorative processes in stored grains of coffee. The treatments 60/40º C and 60º C used to reduce the water content imposed a greater stress condition, accelerated metabolism of natural coffee in the storage with decreased activity of defense mechanisms due to latent damage in these grains. Natural coffees are more sensible to high drying temperatures and its quality reduces faster than pulped coffee in the storage.

  18. Climate and Pest-Driven Geographic Shifts in Global Coffee Production: Implications for Forest Cover, Biodiversity and Carbon Storage

    Science.gov (United States)

    Magrach, Ainhoa; Ghazoul, Jaboury

    2015-01-01

    Coffee is highly sensitive to temperature and rainfall, making its cultivation vulnerable to geographic shifts in response to a changing climate. This could lead to the establishment of coffee plantations in new areas and potential conflicts with other land covers including natural forest, with consequent implications for biodiversity and ecosystem services. We project areas suitable for future coffee cultivation based on several climate scenarios and expected responses of the coffee berry borer, a principle pest of coffee crops. We show that the global climatically-suitable area will suffer marked shifts from some current major centres of cultivation. Most areas will be suited to Robusta coffee, demand for which could be met without incurring forest encroachment. The cultivation of Arabica, which represents 70% of consumed coffee, can also be accommodated in the future, but only by incurring some natural forest loss. This has corresponding implications for carbon storage, and is likely to affect areas currently designated as priority areas for biodiversity. Where Arabica coffee does encroach on natural forests, we project average local losses of 35% of threatened vertebrate species. The interaction of climate and coffee berry borer greatly influences projected outcomes. PMID:26177201

  19. Application of EPR spectroscopy to the examination of pro-oxidant activity of coffee.

    Science.gov (United States)

    Krakowian, Daniel; Skiba, Dominik; Kudelski, Adam; Pilawa, Barbara; Ramos, Paweł; Adamczyk, Jakub; Pawłowska-Góral, Katarzyna

    2014-05-15

    Free radicals present in coffee may be responsible for exerting toxic effects on an organism. The objectives of this work were to compare free radicals properties and concentrations in different commercially available coffees, in solid and liquid states, and to determine the effect of roasting on the formation of free radicals in coffee beans of various origins. The free radicals content of 15 commercially available coffees (solid and liquid) was compared and the impact of processing examined using electron paramagnetic resonance (EPR) spectroscopy at X-band (9.3 GHz). First derivative EPR spectra were measured at microwave power in the range of 0.7-70 mW. The following parameters were calculated for EPR spectra: amplitude (A), integral intensity (I), and line-width (ΔBpp); g-Factor was obtained from resonance condition. Our study showed that free radicals exist in green coffee beans (10(16) spin/g), roasted coffee beans (10(18) spin/g), and in commercially available coffee (10(17)-10(18) spin/g). Free radical concentrations were higher in solid ground coffee than in instant or lyophilised coffee. Continuous microwave saturation indicated homogeneous broadening of EPR lines from solid and liquid commercial coffee samples as well as green and roasted coffee beans. Slow spin-lattice relaxation processes were found to be present in all coffee samples tested, solid and liquid commercial coffees as well as green and roasted coffee beans. Higher free radicals concentrations were obtained for both the green and roasted at 240 °C coffee beans from Peru compared with those originating from Ethiopia, Brazil, India, or Colombia. Moreover, more free radicals occurred in Arabica coffee beans roasted at 240 °C than Robusta. EPR spectroscopy is a useful method of examining free radicals in different types of coffee. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. Weed control in young coffee plantations through post emergence herbicide application onto total area Controle de plantas daninhas em cafezais recém-implantados, com herbicidas aplicados em pós-emergência em área total

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    C.P. Ronchi

    2004-12-01

    Full Text Available This study was carried out to investigate the efficiency of several herbicides under field conditions, by post-emergence application onto the entire area, their effect on the control of weeds in young coffee plantations and commercial coffee and bean intercropping system, as well as on both crops. Seedlings of Coffea arabica cv. Red Catuaí with four to six leaf pairs were transplanted to the field and treated according to conventional agronomic practices. A bean and coffee intercropping system was established by sowing three lines of beans in the coffee inter-rows. At the time the herbicides were sprayed, the coffee plants had six to ten leaf pairs; the bean plants, three leaflets; and the weeds were at an early development stage. Fluazifop-p-butyl and clethodim were selective for coffee plants and controlled only Brachiaria plantaginea and Digitaria horizontalis efficiently. Broad-leaved weeds (Amaranthus retroflexus, Bidens pilosa, Coronopus didymus, Emilia sonchifolia, Galinsoga parviflora, Ipomoea grandifolia, Lepidium virginicum, and Raphanus raphanistrum were controlled with high efficiency by sole applications of fomesafen, flazasulfuron, and oxyfluorfen, except B. pilosa, C. didymus, and R. raphanistrum for oxyfluorfen. Sequential applications in seven-day intervals of fomesafen + fluazifop-p-butyl, or clethodim, and two commercial mixtures of fomesafen + fluazifop-p-butyl simultaneously controlled both types of weed. Cyperus rotundus was only controlled by flazasulfuron. Except for fluazifop-p-butyl and clethodim, all herbicide treatments caused only slight injuries on younger coffee leaves. However, further plant growth was not impaired and coffee plant height and stem diameter were therefore similar in the treatments, as evaluated four months later. Fomesafen, fluazifop-p-butyl, and clethodim, at sole or sequential application, and the commercial mixtures of fomesafen + fluazifop-p-butyl were also highly selective for bean crop; thus

  1. Espresso coffees, caffeine and chlorogenic acid intake: potential health implications.

    Science.gov (United States)

    Crozier, Thomas W M; Stalmach, Angelique; Lean, Michael E J; Crozier, Alan

    2012-01-01

    HPLC analysis of 20 commercial espresso coffees revealed 6-fold differences in caffeine levels, a 17-fold range of caffeoylquinic acid contents, and 4-fold differences in the caffeoylquinic acid : caffeine ratio. These variations reflect differences in batch-to-batch bean composition, possible blending of arabica with robusta beans, as well as roasting and grinding procedures, but the predominant factor is likely to be the amount of beans used in the coffee-making/barista processes. The most caffeine in a single espresso was 322 mg and a further three contained >200 mg, exceeding the 200 mg day(-1) upper limit recommended during pregnancy by the UK Food Standards Agency. This snap-shot of high-street expresso coffees suggests the published assumption that a cup of strong coffee contains 50 mg caffeine may be misleading. Consumers at risk of toxicity, including pregnant women, children and those with liver disease, may unknowingly ingest excessive caffeine from a single cup of espresso coffee. As many coffee houses prepare larger volume coffees, such as Latte and Cappuccino, by dilution of a single or double shot of expresso, further study on these products is warranted. New data are needed to provide informative labelling, with attention to bean variety, preparation, and barista methods.

  2. Efeito de recipientes e substratos utilizados na produção de mudas de cafeeiro no desenvolvimento inicial em casa de vegetação, sob estresse hídrico Effect of different recipients and substrata used in the production of coffee tree seedlings in the initial development in greenhouse under water stress

    Directory of Open Access Journals (Sweden)

    Haroldo Silva Vallone

    2010-04-01

    Full Text Available Com o objetivo de avaliar os efeitos de recipientes e substratos no desenvolvimento inicial de cafeeiros (Coffea arabica L. plantados em vasos dentro de casa de vegetação, sob diferentes níveis de estresse hídrico, foi conduzido um experimento no Setor de Cafeicultura da Universidade Federal de Lavras - UFLA, no período de janeiro a maio de 2004. O delineamento experimental utilizado foi em blocos casualizados (DBC em esquema fatorial (3 x 3 x 4, sendo o primeiro fator constituído de três recipientes utilizados na produção das mudas, tubetes de polietileno rígido de 50 mL; tubetes de 120 mL; e saquinhos de polietileno, nas dimensões de 20 cm de altura por 10 cm de largura e capacidade volumétrica, aproximada, de 700 mL; o segundo fator constituído de três substratos utilizados na produção das mudas, substrato alternativo, composto por 65% de casca de arroz carbonizada + 35% de substrato comercial; substrato comercial Plantmax hortaliças HT; e substrato padrão, composto por 70% de terra + 30% de esterco bovino peneirados e o terceiro fator foi constituído por quatro intervalos entre irrigações, 2, 6, 10 e 14 dias. Observa-se que os recipientes de capacidades volumétricas diferentes e os substratos utilizados influenciam, significativamente, o desenvolvimento dos cafeeiros, sendo que, após 120 dias do transplante das mudas para vasos em casa de vegetação com diferentes níveis de estresse hídrico, as plantas provenientes de mudas produzidas em saquinhos de polietileno e tubetes de 120 mL contendo substratos alternativo e comercial apresentaram melhor desenvolvimento.With the aim of evaluating the effects of different recipients and substrata in the production of coffee tree seedlings (Coffea arabica L., planted in pots inside the greenhouse, under different water stress levels, an experiment was carried out in the Section of Coffee of Department of Agriculture of Universidade Federal de Lavras - UFLA, in the period from

  3. Composição química, produção e qualidade do café fertilizado com diferentes fontes e doses de nitrogênio Chemical composition, yield and quality of the fertilized coffee with different sources and doses of nitrogen

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    Marcelo Ribeiro Malta

    2003-12-01

    Full Text Available Conduziu-se este experimento com a finalidade de se analisar o efeito da adubação nitrogenada sobre a produção e qualidade do café (Coffea arabica L. O delineamento experimental foi em blocos casualizados, no esquema de parcelas subdivididas, utilizando-se seis fontes de nitrogênio (N: calcionamida, nitrato de potássio, nitrocálcio, sulfato de amônio, uréia e nitrato de amônio, nas parcelas, e quatro doses de N (0, 80, 160 e 320 kg ha-1 aplicadas nas subparcelas, com três blocos. Avaliaram-se a produção e as variáveis: atividade da polifenoloxidase, acidez titulável total, açúcares totais, compostos fenólicos totais, ácidos clorogênicos totais, cafeína e prova de xícara. Não houve resposta significativa de produção às fontes e doses de N. Observou-se redução na qualidade do café ao utilizar-se nitrocálcio e nitrato de amônio como fontes de N. Quanto às doses utilizadas, as fontes de N apresentaram efeitos diferenciados em relação à composição química e qualidade do café; entretanto, observou-se, pelas análises da atividade enzimática da polifenoloxidase, acidez titulável total, ácidos clorogênicos totais e compostos fenólicos totais, que doses mais elevadas de sulfato de amônio têm efeito negativo sobre a qualidade dos grãos.This experiment was conducted to analyze the effect of nitrogen fertilization on coffee yield and quality (Coffea arabica L. The experimental design was a randomized complete-block with the split-plot scheme, in which six sources of nitrogen (N were used: calcionamida, potassium nitrate, calcium nitrate, ammonium sulphate, urea and ammonium nitrate in the plots combined with four doses of N (0, 80, 160 and 320 kg ha-1 applied in the subplots, with three replications. Yield and qualitative variables such as polyphenoloxidase activity, total titrable acidity, total sugars, total phenolic compounds, chlorogenic acids, caffeine and sensorial analysis were evaluated. There was no

  4. Optimal conditions for taking spectra of coffee beans plasma spectroscopy induced by laser (LIBS)

    International Nuclear Information System (INIS)

    Diaz Guerrero, A. M.; Flores Reyes; Ponce Cabrera, L. V.

    2016-01-01

    Coffee beans, arabica and robusta, from Mexico (Chiapas and Veracruz), Colombia, Kenya and Sumatra were analyzed by Laser-induced breakdown spectroscopy (LIBS). The time delay and pulse energy were varied in order to find the optimal conditions for taking spectra in coffee beans; finding that the increased visibility of the peaks and the lowest electronic background is observed with 1 s and 450 mJ. Spectra were taken in different regions of grain area to confirm its homogeneous composition. It was found that the intensity of the signal Ca is much higher than that of K in African coffee, lower in the coffee of America, and much lower in the coffee from Asia. (Author)

  5. Spatial estimation of foliar phosphorus in different species of the genus Coffea based on soil properties

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    Samuel de Assis Silva

    2014-10-01

    Full Text Available Information underlying analyses of coffee fertilization systems should consider both the soil and the nutritional status of plants. This study investigated the spatial relationship between phosphorus (P levels in coffee plant tissues and soil chemical and physical properties. The study was performed using two arabica and one canephora coffee variety. Sampling grids were established in the areas, and the points georeferenced. The assessed properties of the soil were levels of available phosphorus (P-Mehlich, remaining phosphorus (P-rem and particle size, and of the plant tissue, phosphorus levels (foliar P. The data were subjected to descriptive statistical analysis, correlation analysis, cluster analysis, and probability tests. Geostatistical and trend analyses were only performed for pairs of variables with significant linear correlation. The spatial variability for foliar P content was high for the variety Catuai and medium for the other evaluated plants. Unlike P-Mehlich, the variability in P-rem of the soil indicated the nutritional status of this nutrient in the plant.

  6. Estudos sôbre a conservação de sementes. IV - Café Influence of moisture content on the viability of coffee seeds

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    Oswaldo Bacchi

    1958-01-01

    Full Text Available Sementes de café, recém-colhidas e imediatamente sêcas à sombra e ao sol, foram conservadas com diferentes teores de umidade em dois tipos de recipientes, abertos e hermèticamente fechados, à temperatura não controlada do laboratório. Pelos resultados obtidos através de determinações periódicas das porcentagens de germinação e umidade das sementes armazenadas nessas condições, foram tiradas as seguintes conclusões: a a vitalidade das sementes foi inteiramente independente do processo de seca; b a longevidade das sementes conservadas em recipientes hermèticamente fechados foi inversamente proporcional ao seu teor de umidade; sementes com aproximadamente 20%, 13% e 10% de umidade premaneceram viáveis durante 4, 8 e 21 meses, respectivamente. c o teor inicial de umidade não teve qualquer influência sôbre a longevidade das sementes armazenadas em recipientes abertos; isto se verificou, provavelmente, em virtude da quantidade relativamente pequena de sementes, o que permitiu uma rápida desidratação daquelas que se encontravam com teores elevados de umidade; a vitalidade das sementes colocadas nestes recipientes permaneceu inalterada por oito a dez meses. Estas conclusões estão inteiramente em desacôrdo com as de vários autores, segundo as quais a semente de café perde rapidamente sua vitalidade pela simples desidratação ao ar.Freshly harvested seeds of coffee (Coffea arabica L. var. typica Cramer were air dried and sun-dried at different levels of moisture content and stored in open and sealed containers at room temperature. Moisture determination and germination tests were made on the "fresh" seeds and at different intervals during the storage period. Based on the results obtained, the following conclusions were drawn: 1 The process of drying did not interfere with the seed viability. 2 The longevity of the seeds kept in sealed containers was inversely proportional to the seed moisture content. Seeds containing about

  7. Dinâmica dos micronutrientes em cafeeiros enxertados Dynamics of micronutrient in grafted coffee

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    André Dominghetti Ferreira

    2013-04-01

    Full Text Available As respostas à disponibilidade dos nutrientes variam entre espécies distintas dentro de um mesmo gênero, por causa, principalmente, das exigências nutricionais variáveis, capacidade de absorção, translocação e utilização dos nutrientes. O objetivo deste trabalho foi avaliar a eficiência de absorção, translocação e uso dos micronutrientes por diferentes cultivares de Coffea arabica L., enxertados em Apoatã IAC 2258 (Coffea canephora. O experimento foi instalado em casa de vegetação, utilizando-se o método de cultivo em solução nutritiva. Foi utilizado um fatorial 7 x 3 + 2, sendo sete cultivares de Coffea arabica L. (Palma II, Catucaí 2 SL, Oeiras MG 6851, Obatã IAC 1669-20, Acauã, Topázio MG 1190 e Paraíso MG H 419-1, três tipos de mudas (pé franco, autoenxertada e enxertada sobre o cultivar Apoatã IAC 2258 e duas testemunhas (Apoatã autoenxertado e Apoatã pé franco. O porta-enxerto utilizado influenciou negativamente na absorção de boro, ferro e manganês. A translocação dos micronutrientes boro e cobre obteve maiores índices nas mudas enxertadas. O cultivar Palma II, quando enxertado, apresentou o maior índice de utilização dos nutrientes, mostrando-se passível de ser enxertado. O porta-enxerto utilizado mostrou-se apto para a enxertia, por não sofrer influência negativa, tanto pela enxertia, quanto pelos cultivares utilizados.Responses to availability of nutrients vary between different species within the same genus, mainly because the different nutritional requirements, absorption capacity, translocation and use of nutrients, The purpose of this study was to evaluate the efficiency of absorption, translocation and use of micronutrients by different cultivars of Coffea arabica L. grafted into Apoatã IAC 2258 (Coffea canephora. The experiment was conducted in a greenhouse using cultivation in nutrient solution in a 7 x 3 + 2 factorial design: seven cultivars of Coffea arabica L. (Palma II, Catuca

  8. Microclimate and development of 'Conilon' coffee intercropped with rubber trees

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    Fábio Luiz Partelli

    2014-11-01

    Full Text Available The objective of this work was to evaluate the influence of intercropping 'Conilon' coffee (Coffea canephora with rubber trees on coffee tree microclimate, nutrition, growth, and yield. Rubber trees were planted in two double rows 33 m apart, with 4x2.3 m spacing between plants. Treatments consisted of the distances from the coffee plants to the rubber trees: 3, 6, 9, 12, and 15 m. Measurements of atmospheric variables (temperature, irradiance, and relative humidity, leaf nutrient concentration, internode length of plagiotropic and orthotropic branches, individual leaf area, chlorophyll content, and yield were performed. Intercropping promotes changes in the microclimatic conditions of coffee plants close to rubber trees, with reduction of temperature and irradiance level and increase in air relative humidity. The proximity of the coffee tree to the rubber trees promotes the elongation of the plagiotropic and orthotropic branches and increases the individual leaf area; however, it does not affect leaf concentrations of N, K, Mg, Fe, Zn, and B in 'Conilon' coffee and does not have a negative impact on yield.

  9. Efeito do tipo de substrato e da presença de adubação suplementar sobre o crescimento vegetativo, nutrição mineral, custo de produção e intensidade de cercosporiose em mudas de cafeeiro formadas em tubetes Effects of substrate type and supplementary fertilization on vegetative growth, mineral nutrition, production costs, and brown eye spot of coffee seedlings in stiff plastic containers

    Directory of Open Access Journals (Sweden)

    Adélia Aziz Alexandre Pozza

    2007-06-01

    Full Text Available A utilização de tubetes para produzir mudas de cafeeiro (Coffea arabica L. tornou-se uma realidade. Objetivando avaliar o uso de substratos adubados ou não com fertilizante de liberação gradual e seus efeitos na nutrição, na formação, na cercosporiose e nos custos de produção de mudas de cafeeiro em tubetes, conduziu-se um experimento em viveiro, em esquema fatorial (4x2 com quatro substratos combinados com duas adubações, tendo quatro repetições. As parcelas foram constituídas por 16 tubetes de 120 mL, considerando-se como plantas úteis as seis centrais. Os tratamentos consistiram dos substratos S1= Substrato comercial I, S2 = Substrato comercial II, S3 = Substrato de eucalipto e S4 = 80% esterco de curral + 20% terra de subsolo, todos com e sem adubação com fertilizante de liberação gradual. No geral, os melhores substratos para formação de mudas de cafeeiro em tubetes foram os substratos não comerciais ou orgânicos, independentemente de receberem ou não adubação suplementar. Observou-se redução da cercosporiose com a nutrição mineral, principalmente com o cálcio. Com a utilização de todos os substratos houve ganho econômico em relação ao custo por milheiro de mudas. A maior economia foi obtida com o substrato S4, seguida do substrato S3, com melhor relação custo/benefício.The utilization of stiff plastic containers for producing coffee seedlings (Coffea arabica L. is a reality. Aiming to evaluate the effects of substrate type enriched or not with slow-release fertilizer on vegetative growth, mineral nutrition, production cost and, brown eye spot of coffee seedlings, an experiment was conducted in the plant nursery. The experimental design was a factorial scheme with four substrates in the presence and absence of supplementary fertilization, with four replicates. The plots were made of 16 containers of 120 ml, using six central plants for evaluation. The treatments consisted of S1 = Commercial Substrate

  10. Effects of maltose and lysine treatment on coffee aroma by flash gas chromatography electronic nose and gas chromatography-mass spectrometry.

    Science.gov (United States)

    He, Yuqin; Zhang, Haide; Wen, Nana; Hu, Rongsuo; Wu, Guiping; Zeng, Ying; Li, Xiong; Miao, Xiaodan

    2018-01-01

    Arabica coffee is a sub-tropical agricultural product in China. Coffee undergoes a series of thermal reactions to form abundant volatile profiles after roasting, so it loses a lot of reducing sugars and amino acids. Adding carbonyl compounds with amino acids before roasting could ensure the nutrition and flavour of coffee. The technology is versatile for the development of coffee roasting process. This investigation evaluates the effects of combining maltose and lysine (Lys) to modify coffee aroma and the possibly related mechanisms. Arabica coffee was pretreated with a series of solvent ratios of maltose and Lys with an identical concentration (0.25 mol L -1 ) before microwave heating. It was found that the combination of maltose and Lys significantly (P ≤ 0.05) influenced quality indices of coffee (pH and browning degree). Ninety-six aromatic volatiles have been isolated and identified. Twelve volatile profiles revealed the relationship between fragrance difference and compound content in coffee. Moreover, coffee aroma was modified by a large number of volatiles with different chemical classes and character. Thus, our results suggest that the combination of reagents changed overall aroma quality through a series of complex thermal reactions, especially the ratio of Lys/maltose over 2:1. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  11. Flavononol Glycosides of Reseda arabica (Resedaceae

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    Djemaa Berrehal

    2012-07-01

    Full Text Available Five flavonol glycosides, kaempferol 3,7-di-O- α -L-rhamnopyranoside (1 , isorhamnetin 3,7-di-O- α -L-rhamnopyranoside (2 , kaempferol 3-O- β -D-glucopyranoside-7-O- α -L-rhamnopyranoside (3 , isorhamnetin 3-O- β -D-glucopyranoside-7-O- α -L-rhamnopyranoside (4, Kaempferol 3-O- β -xylopyranosyl-(1'''→2''-O- α -L-rhamnopyranoside-7-O- α -L-rhamnopyranoside (5, have been isolated from the aerial parts of Reseda arabica. Their structures were established on the basis of physical and spectroscopic analysis, and by comparison with the literature data.

  12. BUSINESS DEVELOPMENT STRATEGY FOR SPECIALTY COFFEE

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    Vharessa Aknesia

    2015-01-01

    Full Text Available Specialty coffee is a coffee of premium quality that has been made through various stages of post-harvest processing and strictly controlled to produce distinctive taste of origins. PT Sinar Mayang Lestari is one of the companies that currently produce and develop specialty coffee type, Arabica Java Preanger. The objectives of the study are to examine competitive advantages and develop an alternative strategy that need to be done by PT Sinar Mayang Lestari for their business development. The research methods used are value chain analysis and VRIO framework to explore competitive advantage owned by the company. The result shows the company currently has a temporary competitive advantage of the technological resources and reputation. By using SWOT-AHP technique, the alternative strategies that can be done by company are as follows: 1 increasing the production of natural and honey coffee  type; 2 building coffee center in plantation site for sharing knowledge and innovation media to the farmers; 3 improving the competency of human resource in plantation, post harvest, and promoting area; 4 building management system gradually 5 forwarding integration by building roast and ground coffee business; and 6 maximizing the ability of the land and human resources through research and development.Keywords: competitive advantage, specialty coffee, SWOT-AHP, value chain, VRIOABSTRAKKopi special merupakan kopi dengan kualitas premium yang sudah melalui berbagai tahapan pengolahan pascapanen yang diawasi dengan ketat sehingga menghasilkan cita rasa yang khas sesuai dengan daerah asalnya. PT Sinar Mayang Lestari adalah salah satu perusahaan yang saatini memproduksi dan mengembangkan kopi spesial jenis Arabika Java Preanger. Tujuan dari penelitian ini adalah menganalisis keunggulan bersaing yang dimiliki dan mengembangkan alternative strategi yang perlu dilakukanoleh PT Sinar Mayang Lestari untuk pengembangan usahanya. Penelitian ini menggunakan analisis rantai

  13. Melhoramento do cafeeiro: XIII - Café bourbon amarelo Coffee breeding: XIII - yellow bourbon coffee

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    A. Carvalho

    1957-01-01

    ocorrência desse tipo de plantas é de 1,7% no Bourbon Amarelo, inferior à que se encontra no café Mundo Novo. Os dados referentes às porcentagens de sementes dos tipos moca e concha indicaram que não há excesso de sementes anormais. As sementes moca mostraram-se mais freqüentes em Ribeirão Prêto, e menos em Pindorama e Campinas. As sementes concha são mais freqüentes em Campinas e Pindorama, e menos em Ribeirão Prêto. Quanto à distribuição das porcentagens de sementes concha, notou-se maior variação em Campinas e Pindorama. Com relação à ocorrência da anomalia constituída por frutos com lojas desenvolvidas e sem sementes, verificou-se que é pouco freqüente no café Bourbon Amarelo e da mesma intensidade que a verificada em outras variedades comerciais, como Bourbon Vermelho e Caturra. A ocorrência de plantas com frutos alaranjados, heterozigotas para os alelos Xcxc, indicou que devem ser freqüentes os cruzamentos naturais no cafezal onde as plantas matrizes foram selecionadas, pois embora o número de plantas de frutos vermelhos (XcXc no local fôsse de freqüência reduzida em relação às plantas de frutos amarelos (xcxc, a ocorrência média de plantas heterozigotas entre os descendentes foi de 2,46%. Devido às características apresentadas por êsse grupo de progênies analisadas, tais como vigor vegetativo, alta produção e bom rendimento, confirma-se ser o Bourbon Amarelo uma variedade de café de bastante importância econômica, devendo suas seleções ser cultivadas em larga escala.Little is known about the origin of Yellow Bourbon coffee (Coffea arabica cv. Bourbon Amarelo, one of the most promising coffee varieties. It probably originated in the neighbourhood of Pederneiras, S. P. (Brazil, either as a mutation of Bourbon coffee (red fruits, or as a recombinant from an eventual natural hybridization between this variety and Amarelo de Botucatu (yellow fruits when ripe, which is a natural mutation of typica (C. arabica L. var

  14. Comportamiento productivo de clones de café robusta (Coffea Canephora p en Manglaralto, Ecuador.

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    Mercedes Arzube Mayorga

    2017-05-01

    Abstract The research was carried out in the experimental farm Manglaralto, owned by the Peninsula State University of Santa Elena, located at the coordinates UTM 528944m E and 9796468m S zone 17M datum WGS-84 at 12 msnm, with topography of less than 1%, research (Coffea canephora P., high productivity in the agroecological conditions of Manglaralto Ecuador. For the establishment of the trial, 23 clones of robust coffee, selected by COFENAC in the Amazon region of northern Ecuador, were used. The clones were arranged randomly, each clone is an experimental unit represented by 20 plants, planted at a distance of 3 x 3 meters. Prelimi