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Sample records for coffea lasalle hymenoptera

  1. Susceptibility of the Parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) to Beauveria bassiana under laboratory conditions; Susceptibilidad del parasitoide Phymastichus coffea LaSalle (Hymenoptera:Eulophidae) a Beauveria bassiana en condiciones de laboratorio

    Energy Technology Data Exchange (ETDEWEB)

    Castillo, Alfredo; Gomez, Jaime; Infante, Francisco [El Colegio de la Frontera Sur (ECOSUR), Chiapas (Mexico). Dept. de Entomologia Tropical], e-mail: acastill@ecosur.mx, e-mail: jgomez@ecosur.mx, e-mail: finfante@ecosur.mx; Vega, Fernando E. [United States Department of Agriculture (USDA), Beltsville, MD (United States). Agricultural Research Service. Sustainable Perennial Crops Lab.], e-mail: fernando.vega@ars.usda.gov

    2009-09-15

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is the most important coffee pest worldwide. Beauveria bassiana is a generalist entomopathogenic fungus widely used by coffee farmers to control this pest and Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) is an African endo parasitoid of H. hampei adults, recently imported to several Latin American and Caribbean countries to aid in the coffee berry borer control. The objective of this study was to determine if B. bassiana is detrimental to P. coffea. The susceptibility of the parasitoid was evaluated in terms of adult survivorship, mean lethal concentration (LC{sub 50}), mean lethal time (LT{sub 50}), reproduction and immature mortality. The main effect of the fungus resulted in reduction of adult longevity and mortality of 100% for immature stages of this parasitoid. The LC{sub 50} for adults was 0.11% equivalent to 9.53 x 10{sup 7} conidia/ml of B. bassiana and a LT{sub 50} of 29.4 h, equivalent to reduction of 22% of its normal longevity as an adult. P. coffea was capable of disseminating spores of B. bassiana to non-infected H. hampei adults, which could indirectly cause the death of its own progeny. These results could be valuable when considering the use of both organisms in the field, especially in an integrated pest management program. (author)

  2. Susceptibility of the Parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) to Beauveria bassiana under laboratory conditions

    International Nuclear Information System (INIS)

    Castillo, Alfredo; Gomez, Jaime; Infante, Francisco; Vega, Fernando E.

    2009-01-01

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is the most important coffee pest worldwide. Beauveria bassiana is a generalist entomopathogenic fungus widely used by coffee farmers to control this pest and Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) is an African endo parasitoid of H. hampei adults, recently imported to several Latin American and Caribbean countries to aid in the coffee berry borer control. The objective of this study was to determine if B. bassiana is detrimental to P. coffea. The susceptibility of the parasitoid was evaluated in terms of adult survivorship, mean lethal concentration (LC 50 ), mean lethal time (LT 50 ), reproduction and immature mortality. The main effect of the fungus resulted in reduction of adult longevity and mortality of 100% for immature stages of this parasitoid. The LC 50 for adults was 0.11% equivalent to 9.53 x 10 7 conidia/ml of B. bassiana and a LT 50 of 29.4 h, equivalent to reduction of 22% of its normal longevity as an adult. P. coffea was capable of disseminating spores of B. bassiana to non-infected H. hampei adults, which could indirectly cause the death of its own progeny. These results could be valuable when considering the use of both organisms in the field, especially in an integrated pest management program. (author)

  3. Field-cage evaluation of the parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) as a natural enemy of the coffee berry borer

    Science.gov (United States)

    Phymastichus coffea (Hymenoptera: Eulophidae) is an African parasitoid that has been imported to Mexico and other Latin American countries for the biological control of the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae). As a part of the evaluation of this ...

  4. Palmistichus elaeisis Delvare & LaSalle (Hymenoptera, Eulophidae: a new parasitoid of Dione juno juno (Cramer (Lepidoptera, Nymphalidae Palmistichus elaeisis Delvare & LaSalle (Hymenoptera, Eulophidae: um novo parasitóide de Dione juno juno (Cramer (Lepidoptera, Nymphalidae

    Directory of Open Access Journals (Sweden)

    Hélcio R. Gil-Santana

    2006-09-01

    Full Text Available Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera, Eulophidae is recorded as parasitoid of Dione juno juno (Cramer, 1779 (Lepidoptera, Nymphalidae in the State of Rio de Janeiro, Brazil.Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera, Eulophidae é registrado como parasitóide de Dione juno juno (Cramer, 1779 (Lepidoptera, Nymphalidae, no estado do Rio de Janeiro, Brasil.

  5. Reproductive performance of Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) with previously refrigerated pupae of Bombyx mori L. (Lepidoptera: Bombycidae)

    OpenAIRE

    Pereira, FF.; Zanuncio, JC.; Serrão, JE.; Pastori, PL.; Ramalho, F.S.

    2009-01-01

    The mass rearing of parasitoids represents a fundamental stage for programmes of biological control. The progeny of the parasitoid Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) were evaluated on previously refrigerated pupae of Bombyx mori L. (Lepidoptera: Bombycidae). Forty-eight to 72 hours-old pupae of B. mori were stored at 10 ºC for five, 10, 15 or 20 days and then exposed to parasitism by P. elaeisis females. This parasitoid showed shorter duration of the life cycl...

  6. Reproductive performance of Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae with previously refrigerated pupae of Bombyx mori L. (Lepidoptera: Bombycidae

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    FF. Pereira

    Full Text Available The mass rearing of parasitoids represents a fundamental stage for programmes of biological control. The progeny of the parasitoid Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae were evaluated on previously refrigerated pupae of Bombyx mori L. (Lepidoptera: Bombycidae. Forty-eight to 72 hours-old pupae of B. mori were stored at 10 ºC for five, 10, 15 or 20 days and then exposed to parasitism by P. elaeisis females. This parasitoid showed shorter duration of the life cycle when reared on pupae of B. mori which were previously stored at 10 ºC during 15 days. P. elaeisis parasitized 100% of the pupae of B. mori after storage at 10 ºC during all periods with emergence of this parasitoid from 78 to 100% of these pupae. P. elaeisis had a higher number of progeny per pupa of B. mori stored for 15 days at 10 ºC. Pupae of B. mori can be stored for 15 days at 10 ºC before being used to rear P. elaeisis.

  7. The density of females of Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) affects their reproductive performance on pupae of Bombyx mori L. (Lepidoptera: Bombycidae).

    Science.gov (United States)

    Pereira, Fabricio F; Zanuncio, José C; Serrão, José E; Zanuncio, Teresinha V; Pratissoli, Dirceu; Pastori, Patrik L

    2010-06-01

    Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) is a gregarious and polyphagous parasitoid mainly of Lepidoptera pupae. The objective of this paper as to study the developent of parasitoid on Bombyx mori L. (Lepidoptera: Bombycidae) pupae exposed to one, nine, 18, 27, 36, 45 or 54 female P. elaeisis, respectively. The females of the parasitoid remained in contact with pupae for 24 hours in glass tubes (14.0 x 2.2 cm), packed in a climatic chamber regulated at 25 +/- 2 degrees C, 70 +/- 10% relative humidity and photo phase of 12 hours. With the exception of density 1:1 (72.72%), in other densities parasitism was 100%. Adults of P. elaeisis did not emerge from pupae at densities of 1:1 and 9:1, but 100.0% of parasitoid emergence was observed at the density of 45:1 and 54.54% at 54:1. The duration of the life cycle of this parasitoid ranged from 20 to 28 days. P. elaeisis produced 49 to 589 descendants per pupa of B. mori. The sex ratio of P. elaeisis ranged from 0.93 +/- 0.01 to 0.97 +/- 0.01 without differences with 18, 27, 36, 45 and 54 females/host. This parasitoid should be reared with the density of 45 females per pupa of B. mori.

  8. Reproductive performance of Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) with previously refrigerated pupae of Bombyx mori L. (Lepidoptera: Bombycidae).

    Science.gov (United States)

    Pereira, F F; Zanuncio, J C; Serrão, J E; Pastori, P L; Ramalho, F S

    2009-08-01

    The mass rearing of parasitoids represents a fundamental stage for programmes of biological control. The progeny of the parasitoid Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) were evaluated on previously refrigerated pupae of Bombyx mori L. (Lepidoptera: Bombycidae). Forty-eight to 72 hours-old pupae of B. mori were stored at 10 degrees C for five, 10, 15 or 20 days and then exposed to parasitism by P. elaeisis females. This parasitoid showed shorter duration of the life cycle when reared on pupae of B. mori which were previously stored at 10 degrees C during 15 days. P. elaeisis parasitized 100% of the pupae of B. mori after storage at 10 degrees C during all periods with emergence of this parasitoid from 78 to 100% of these pupae. P. elaeisis had a higher number of progeny per pupa of B. mori stored for 15 days at 10 degrees C. Pupae of B. mori can be stored for 15 days at 10 degrees C before being used to rear P. elaeisis.

  9. Efecto de la dieta artificial MP sobre la emergencia y relacion de sexos de Phymastichus coffea (Hymenoptera:Eulophidae) mantenido sobre su hueped, Hypothenemus hampei (Coleoptera: Scloytidae)a traves de generaciones contin

    Science.gov (United States)

    Phymastichus coffea La Salle (Hymenoptera: Eulophidae) is an endoparasitoid that attacks the adult coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Scolytidae). The MP diet developed by Portilla and Streett is the only reported diet that allows cultures of P. coffea to develop and repr...

  10. The density of females of Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae affects their reproductive performance on pupae of Bombyx mori L. (Lepidoptera: Bombycidae

    Directory of Open Access Journals (Sweden)

    Fabricio F. Pereira

    2010-06-01

    Full Text Available Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae is a gregarious and polyphagous parasitoid mainly of Lepidoptera pupae. The objective of this paper as to study the developent of parasitoid on Bombyx mori L. (Lepidoptera: Bombycidae pupae exposed to one, nine, 18, 27, 36, 45 or 54 female P. elaeisis, respectively. The females of the parasitoid remained in contact with pupae for 24 hours in glass tubes (14.0 x 2.2 cm, packed in a climatic chamber regulated at 25 ± 2°C, 70 ± 10% relative humidity and photo phase of 12 hours. With the exception of density 1:1 (72.72%, in other densities parasitism was 100%. Adults of P. elaeisis did not emerge from pupae at densities of 1:1 and 9:1, but 100.0% of parasitoid emergence was observed at the density of 45:1 and 54.54% at 54:1. The duration of the life cycle of this parasitoid ranged from 20 to 28 days. P. elaeisis produced 49 to 589 descendants per pupa of B. mori. The sex ratio of P. elaeisis ranged from 0.93 ± 0.01 to 0.97 ± 0.01 without differences with 18, 27, 36, 45 and 54 females/host. This parasitoid should be reared with the density of 45 females per pupa of B. mori.Palmistichus elaeisis Delvare e LaSalle (Hymenoptera: Eulophidae é um parasitóide polífago, que inviabiliza, principalmente, pupas de lepidópteros. O objetivo desse trabalho foi estudar o desenvolvimento do parasitóide em pupas de Bobyx mori L. (Lepidoptera: Bombycidae expostas a uma, nove, 18, 27, 36, 45 ou 54 fêmeas de P. elaeisis, respectivamente. As fêmeas do parasitóide permaneceram em contato com as pupas por 24 horas em tubos de vidro (14,0 x 2,2 cm, acondicionadas em câmara climatizada regulada a 25 ± 2°C, 70 ± 10% de UR e fotofase de 12 horas. Com exceção da densidade 1:1 (72,72%, nas demais densidades o parasitismo foi 100%. Adultos de P. elaeisis não emergiram nas densidades de 1:1 e 9:1, mas observou-se 100% de emergência do parasitóide na densidade 45:1 e 54,54% em 54:1. A dura

  11. Progênie de Palmistichus elaeisis Delvare & LaSalle (Hymenoptera: Eulophidae) parasitando pupas de Bombyx mori L. (Lepidoptera: Bombycidae) de diferentes idades

    OpenAIRE

    Pereira, Fabricio F; Zanuncio, José C; Serrão, José E; Oliveira, Harley N; Fávero, Kellen; Grance, Elizangela L V

    2009-01-01

    Palmistichus elaeisis Delvare & LaSalle é um parasitóide pupal natural de pupas de lepidópteros desfolhadores de eucalipto e é considerado um promissor agente de controle biológico. No entanto, o desenvolvimento de técnicas de criação eficientes desse inimigo natural é inicialmente necessário para que ele possa ser utilizado em programas de controle biológico. Pupas de Bombyx mori L. são hospedeiras alternativas em potencial para criação massal de P. elaeisis. Por isto, nós avaliamos a idade ...

  12. Progeny of Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) parasitising pupae of Bombyx mori L. (Lepidoptera: Bombycidae) of different ages

    International Nuclear Information System (INIS)

    Pereira, Fabricio F.; Favero, Kellen; Grance, Elizangela L.V.

    2009-01-01

    Palmistichus elaeisis Delvare and LaSalle is a natural pupal parasitoid of eucalyptus defoliator lepidopterans and is considered a promising biocontrol agent. However, the development of efficient rearing techniques for this natural enemy are fi rst required before it can be used in biocontrol programs. Bombyx mori L. pupae are potential alternative hosts for this parasitoid mass rearing, and they are easy to rear. Therefore, we investigated the most suitable host age and the effects of parasitoid age on progeny production of P. elaeisis. B. mori pupae, 24 h-, 48 h-, 72 h- or 96 h-old were exposed to P. elaeisis females of similar age. The duration of the life cycle (egg-adult) of P. elaeisis was not affected by the age of the parasitizing female; however, the host age affected parasitoid development. The best parasitization was obtained for 72h- to 96h-old parasitoid females when offered to 48h- to 72h-old host pupae, allowing the synchronized rearing of a large number of P. elaeisis offspring. (author)

  13. [Progeny of Palmistichus elaeisis Delvare & LaSalle (Hymenoptera: Eulophidae) parasitising pupae of Bombyx mori L. (Lepidoptera: Bombycidae) of different ages].

    Science.gov (United States)

    Pereira, Fabricio F; Zanuncio, José C; Serrão, José E; Oliveira, Harley N; Fávero, Kellen; Grance, Elizangela L V

    2009-01-01

    Palmistichus elaeisis Delvare & LaSalle is a natural pupal parasitoid of eucalyptus defoliator lepidopterans and is considered a promising biocontrol agent. However, the development of efficient rearing techniques for this natural enemy are first required before it can be used in biocontrol programs. Bombyx mori L. pupae are potential alternative hosts for this parasitoid mass rearing, and they are easy to rear. Therefore, we investigated the most suitable host age and the effects of parasitoid age on progeny production of P. elaeisis. B. mori pupae, 24 h-, 48 h-, 72 h- or 96 h-old were exposed to P. elaeisis females of similar age. The duration of the life cycle (egg-adult) of P. elaeisis was not affected by the age of the parasitizing female; however, the host age affected parasitoid development. The best parasitization was obtained for 72 h- to 96 h-old parasitoid females when offered to 48 h- to 72 h-old host pupae, allowing the synchronized rearing of a large number of P. elaeisis offspring.

  14. Progeny of Palmistichus elaeisis Delvare and LaSalle (Hymenoptera: Eulophidae) parasitising pupae of Bombyx mori L. (Lepidoptera: Bombycidae) of different ages; Progenie de Palmistichus elaeisis Delvare e LaSalle (Hymenoptera:Eulophidae) parasitando pupas de Bombyx mori L. (Lepidoptera:Bombycidae) de diferentes idades

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Fabricio F.; Favero, Kellen; Grance, Elizangela L.V. [Universidade Federal da Grande Dourados, MS (Brazil). Fac. de Ciencias Biologicas e Ambientais], e-mail: fabriciofagundes@ufgd.edu.br, e-mail: kellenfavero@yahoo.com.br, e-mail: eli_vargasgrance@yahoo.com.br; Zanuncio, Jose C. [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Biologia Animal], e-mail: zanuncio@ufv.br; Serrao, Jose E. [Universidade Federal de Vicosa (UFV), MG (Brazil), Dept. de Biologia Geral], e-mail: jeserrao@ufv.br; Oliveira, Harley N. [Embrapa Agropecuaria Oeste, Dourados, MS (Brazil)], e-mail: harley@cpao.embrapa.br

    2009-09-15

    Palmistichus elaeisis Delvare and LaSalle is a natural pupal parasitoid of eucalyptus defoliator lepidopterans and is considered a promising biocontrol agent. However, the development of efficient rearing techniques for this natural enemy are fi rst required before it can be used in biocontrol programs. Bombyx mori L. pupae are potential alternative hosts for this parasitoid mass rearing, and they are easy to rear. Therefore, we investigated the most suitable host age and the effects of parasitoid age on progeny production of P. elaeisis. B. mori pupae, 24 h-, 48 h-, 72 h- or 96 h-old were exposed to P. elaeisis females of similar age. The duration of the life cycle (egg-adult) of P. elaeisis was not affected by the age of the parasitizing female; however, the host age affected parasitoid development. The best parasitization was obtained for 72h- to 96h-old parasitoid females when offered to 48h- to 72h-old host pupae, allowing the synchronized rearing of a large number of P. elaeisis offspring. (author)

  15. The LaSalle probabilistic safety analysis

    International Nuclear Information System (INIS)

    Frederick, L.G.; Massin, H.L.; Crane, G.R.

    1987-01-01

    A probabilistic safety analysis has been performed for LaSalle County Station, a twin-unit General Electric BWR5 Mark II nuclear power plant. A primary objective of this PSA is to provide engineers with a useful and useable tool for making design decisions, performing technical specification optimization, evaluating proposed regulatory changes to equipment and procedures, and as an aid in operator training. Other objectives are to identify the hypothetical accident sequences that would contribute to core damage frequency, and to provide assurance that the total expected frequency of core-damaging accidents is below 10 -4 per reactor-year in response to suggested goals. (orig./HSCH)

  16. Potencial de Formigas (Hymenoptera: Formicidae como Agentes Anti-herbívoros em Cultivo de Café (Coffea canephora Pierre e Feijão Guandu [Cajanus cajans (L. Millsp

    Directory of Open Access Journals (Sweden)

    Leandro Chevalier

    2013-07-01

    Abstract. This study analyzed the role of ants as anti-herbivore agents in Coffee (Coffea canephora Pierre and Pigeon Pea [Cajanus cajans (L. Millsp] plants in agroecosystem under organic management. In these plants we used artificial eggs and sugar solution in experiments that simulated the presence of herbivore insects. Coffee plants did not possess any natural attractive that could intensify foraging activity of ants, but pigeon pea plants were infested by treehoppers which attracted ants. Activity of ants was surveyed after sugar solution application and artificial eggs removal was measured 24 and 48 h after start of the experiments. Sugar solution sprinkled on plants foliage increased significantly the activity of ants on coffee and pigeon pea plants. The removal of artificial eggs was significantly greater in branch with ants than without ants for pigeon pea plants. The sugar solution addiction resulted in greater artificial eggs removal in pigeon pea plants, but not in coffee plants. The treatment with sugar solution and presence of treehoppers had similar effects on the activity of ants on the plants. This study showed that the application of sugar solution can be used to increase activity of ants on plants as well as the potential of ants as anti-herbivore agents can be evaluated in experiments that simulate the presence of insects on the plants.

  17. AMELIORATON GENETIQUE DE Coffea canephora Pierre PAR ...

    African Journals Online (AJOL)

    AISA

    Mots clés : Café, descendance, robusta, C. canephora, congusta, Côte d'Ivoire. ABSTRACT. GENETIC BREEDING OF coffea canephoraPierre BY INTERSPECIFIC HYBRIDIZATION : STUDY OF FIRST GENERATION. HYBRIDES OF coffea cannephoraPierre X coffea congensis Froenhner. Coffee represents one of the most ...

  18. Controlling Chaos in Neuron Based on Lasalle Invariance Principle

    International Nuclear Information System (INIS)

    Wei Duqu; Qin Yinghua

    2011-01-01

    A new control law is proposed to asymptotically stabilize the chaotic neuron system based on LaSalle invariant principle. The control technique does not require analytical knowledge of the system dynamics and operates without an explicit knowledge of the desired steady-state position. The well-known modified Hodgkin-Huxley (MHH) and Hindmarsh-Rose (HR) model neurons are taken as examples to verify the implementation of our method. Simulation results show the proposed control law is effective. The outcome of this study is significant since it is helpful to understand the learning process of a human brain towards the information processing, memory and abnormal discharge of the brain neurons. (general)

  19. Hymenoptera: Formicidae

    African Journals Online (AJOL)

    1990-05-15

    May 15, 1990 ... Supplemental Library User's Guide 1983 Edition. pp. 131-144. (Ed.) S. Ioyner. SAS Institute Incorporated, Cary,. North Carolina. CORBARA, B., LACHAUD, I. & FRESNEAU, D. 1989. Individual variability, social structure and division of labour in the ponerine ant, Ectatommo. ruidum Roger (Hymenoptera,.

  20. Channel Control-Blade Interference Management at LaSalle 1 and 2 during 2007 and 2008

    Energy Technology Data Exchange (ETDEWEB)

    Cantonwine, Paul; Crawford, Doug; Downs, Mike [Global Nuclear Fuels, PO Box 780, Wilmington, NC 28402 (United States); Joe, Bertrum [GE-Hitachi, 1989 Little Orchard St., San Jose, CA 95125-1030 (United States); Bahensky, Ted [GE-Hitachi, PO Box 780, Wilmington, NC 28402 (United States); Reimer, John [Exelon Nuclear, 2601 North 21st Road, Marseilles, Il 61341-9757 (United States); Hoz, Carlos del la; Petersen, Ken [Exelon Nuclear, 4300 Winfield Road, Warrenville, IL 60555 (United States); Reitmeyer, Mike [Exelon Nuclear, 200 Exelon Way, Kennett Square, PA 19348 (United States); Morris, Jeff; Zbib, Ali [AREVA NP, 2101 Horn Rapids Road, Richland, WA. 99354 (United States)

    2009-06-15

    This paper provides a summary of the operational experience at LaSalle 1 and LaSalle 2 regarding channel control-blade interference that occurred in 2007 and 2008. Channel distortion data from LaSalle 1 provides a characterization of distortion in all four bundles in cells that experienced channel interference and cells that did not. Also, this paper provides a new channel distortion management strategy implemented at LaSalle 2 that avoided a mid-cycle outage. LaSalle 1 and LaSalle 2 are GE designed Boiling Water Reactors (BWR/5 Type) that generate 1195 MW electric. During 2007 and 2008, each core had 1. and 3. Cycle AREVA ATTRIUM{sup TM} 10 fuel with 100 mil Zr-2 channels and 2. Cycle GNF GE14 fuel with 120/75 mil Zr-2 channels. As a result of the channel control-blade interference observed in 2007 and 2008, two peripheral cells in LaSalle 1 and two (1 peripheral and 1 interior) cells in LaSalle 2 were declared inoperable. The first observations of channel control-blade friction occurred in September 2007 in LaSalle 1 about 6 months prior to the end of a 2-year cycle. LaSalle 2 had started up approximately 6 months earlier and had 18 months left the cycle. The initial observations (eventually seven cells with no-settle conditions were observed in LaSalle) were limited to the peripheral cells where fluence gradient-induced bow was the dominant distortion mechanism. However, near the end of cycle in LaSalle 1 in January 2008, a number of interior cells were unexpectedly found to not settle. These were later determined to be a result of shadow corrosion-induced bow. Further testing to determine the extent of condition found a total of nine interior cells that failed the no-settle criterion. These unexpected observations instigated a significant response that resulted in an extensive expansion of the work scope for the upcoming outage that began on February 4, 2008. Specifically, a large channel measurement campaign and a large re-channeling campaign were added. The

  1. Volatile compounds profiles in unroasted Coffea arabica and Coffea canephora beans from different countries

    Directory of Open Access Journals (Sweden)

    Daniel KNYSAK

    Full Text Available Abstract Aroma is the most important factor in assessing the quality of coffee. The volatile compounds profile could be very important to confirm the authenticity of Coffea arabica. The study was carried out on two species of unroasted coffee beans: Coffea arabica from Colombia and Nepal and Coffea robusta from Uganda and Vietnam. Both Coffea arabica and Coffea canephora were imported to the country of analysis approximately 5 months prior to the research. Before the analysis, the coffee beans were kept in a sealed, dark container, at 21 °C. The tests were performed using an electronic nose. Its functioning is based on gas chromatography with two columns of different polarities in parallel and with 2 ultra sensitive Flame Ionization Detectors (FID. With multivariate statistics – Principal Components Analysis – it was possible to reduce the number of links and present them in two dimensions, which allowed for the unambiguous identification and assignment of samples to a particular species of coffee. By using an electronic nose, one can distinguish and group unroasted coffee beans’ flavours depending on the country of origin and species.

  2. Seletividade de inseticidas recomendados para a cultura do algodão ao parasitóide de pupas Palmistichus elaeisis (Hymenoptera: Eulophidae)

    OpenAIRE

    Barbosa, Wagner Faria

    2010-01-01

    Insetos pragas podem reduzir a produção do algodoeiro e o controle biológico pode reduzir o uso excessivo de inseticidas nessa cultura. O endoparasitóide gregário de pupas de lepidópteros e coleópteros Palmistichus elaeisis Delvare & LaSalle (Hymenoptera: Eulophidae), por ter hábito generalista e facilidade de criação em hospedeiro alternativo, pode ser utilizado no controle biológico de pragas do algodoeiro. O objetivo dessa pesquisa foi estudar o impacto dos inseticidas acefato, cartape, cl...

  3. Simulations of the recent LaSalle-2 incident with the BNL plant analyzer

    International Nuclear Information System (INIS)

    Cheng, H.S.; Mallen, A.N.; Wulff, W.

    1989-01-01

    This paper presents the results of simulations of the recent power oscillation incident at the LaSalle-2 nuclear power plant using the BNL plant analyzer. The causes of the oscillation were investigated and the sensitivity of the oscillation to key parameters was studied. It is concluded that the observed power oscillation was caused by boiling instability (i.e., density wave oscillation) reinforced by the reactivity feedback in neutron kinetics, and that the density wave oscillation resulted from flow reduction due to recirculation pump trip and feedwater temperature reduction due to partial loss of feedwater heating capability as well as power peaking

  4. Fire environment determination in the LaSalle NPP control room

    Energy Technology Data Exchange (ETDEWEB)

    Usher, J.L.; Boccio, J.L.; Singhal, A.K.; Tam, L.T.

    1986-01-01

    One objective of NRC's Fire Protection Research Program (FPRP) is to improve the modeling of environments caused by fires in typical nuclear power plant enclosures. A three-dimensional fluid dynamics computer code (PHOENICS) has been adapted as a field-model fire code (SAFFIRE) for this purpose. The model has been applied to simulate two distinct fires in the control room of the LaSalle County power plant. The environments determined illustrate hazardous potential for both personnel and equipment.

  5. Uma nova forma de Coffea

    Directory of Open Access Journals (Sweden)

    C. A. Krug

    1950-01-01

    Full Text Available Nos extensos trabalhos de melhoramento do cafeeiro, há 18 anos em realização na Subdivisão de Genética do Instituto Agronômico, tem-se dedicado especial atenção à espécie C. arabica L., pelo fato de todos os nossos cafèzais pertencerem a esta espécie que, sem dúvida, fornece o produto de melhor qualidade. Nas regiões de terras extremamente cansadas, um dos principais fatôres levados em consideração no melhoramento é a rusticidade, caráter êsse, entretanto, encontrado de preferência em outras espécies, tais como o C. canephora e C. Dewevrei, cujos cafés são de má qualidade. A hibridação interespecífica, que poderia reunir em uma só planta caraterísticos de rusticidade e boa qualidade de bebida, tem o inconveniente de dar origem a plantas triplóides, que são estéreis. Daí se deduz que a obtenção artificial de formas que combinassem êsses caraterísticos constitui problema, cuja solução é extremamente demorada. No presente trabalho, apresentam-se os caracteres de uma nova forma de Coffea, encontrada em cafèzal da Fazenda Itaporã, em Terra Roxa, município de Viradouro, que, com algumas ressalvas, oferece a desejada combinação de caracteres. Trata-se, provàvelmente, de um híbrido espontâneo entre C. arabica e C. Dewevrei, com 2n = 44 cromosômios, extremamente rústico e produtivo, cujas sementes fornecem uma bebida que pode ser classificada como boa. Apenas apresenta, como principal defeito, uma auto-esterilidade quase completa. Os seus caraterísticos botânicos são descritos em detalhe. Devido ao seu porte elevado, ramos abundantes e folhas grandes e coriáceas, esse cafeeiro se assemelha ao C. Dewevrei. Os frutos são oval-elípticos, de um vermelho bem escuro quando maduros, e as sementes oblongas, constatan-do-se elevada percentagem do tipo "moca" e "chocha". Quanto à constituição citológica, as pesquisas conduziram à hipótese de este cafeeiro possuir 22 cromosômios de C. arabica e 22 (n

  6. Integrated Level 3 risk assessment for the LaSalle Unit 2 nuclear power plant

    International Nuclear Information System (INIS)

    Payne, A.C. Jr.; Brown, T.D.; Miller, L.A.

    1991-01-01

    An integrated Level 3 probabilistic risk assessment (PRA) was performed on the LaSalle County Station nuclear power plant using state-of-the-art PRA analysis techniques. The objective of this study was to provide an estimate of the risk to the offsite population during full power operation of the plant and to include a characterization of the uncertainties in the calculated risk values. Uncertainties were included in the accident frequency analysis, accident progression analysis, and the source term analysis. Only weather uncertainties were included in the consequence analysis. In this paper selected results from the accident frequency, accident progression, source term, consequence, and integrated risk analyses are discussed and the methods used to perform a fully integrated Level 3 PRA are examined. LaSalle County Station is a two-unit nuclear power plant located 55 miles southwest of Chicago, Illinois. Each unit utilizes a Mark 2 containment to house a General Electric 3323 MWt BWR-5 reactor. This PRA, which was performed on Unit 2, included internal as well as external events. External events that were propagated through the risk analysis included earthquakes, fires, and floods. The internal event accident scenarios included transients, transient-induced LOCAs (inadvertently stuck open relief valves), anticipated transients without scram, and loss of coolant accidents

  7. Water chemistry and radiation buildup at the Commonwealth Edison Company LaSalle-1 BWR. Final report

    International Nuclear Information System (INIS)

    Earls, C.E.; Blok, J.

    1986-09-01

    This report presents the results of a comprehensive study of the water quality and radiation buildup at the LaSalle County Unit 2 boiling warer reactor (BWR). The purpose of the study was to determine the effect of corrosion product inputs from the forward pumped heater drains on overall water quality. Since the drains are pumped into the feedwater line without filtration or demineralization, corrosion products in these streams will directly add to the impurity levels of the final feedwater. At LaSalle, the forward pumped heater drains contributed less to the feedwater impurities, on average, than the effluent of the condensate demineralizer. The feedwater quality at LaSalle was generally in the ''acceptable'' range. Nevertheless, significant water chemistry improvements, especially in reducing the corrosion product spikes associated with power or flow transients, is highly desirable for this plant. Such improvements should begin with a more consistent quality of demineralizer operation. Quantitative gamma scans of the primary system piping at LaSalle 2 were carried out in the course of the water chemistry study. Although the cumulative operational exposure of the plant was relatively limited at the time this study was carried out, the radiation buildup rate did appear to be rapid (in fact, among the most rapid) compared to other similar BWRs

  8. Energy savings program in Ville LaSalle; Programme d'economies d'energie a Ville LaSalle

    Energy Technology Data Exchange (ETDEWEB)

    Savard, M. [Ville de Montreal, PQ (Canada). Public Works

    2002-09-30

    In 1998, City of LaSalle implemented an energy efficiency program for two municipal buildings. The first building is a sports complex which comprises a swimming pool, an arena, areas for boxing, squash, racket ball, as well as a reception area. The second building is City Hall, which comprises two buildings, one with two storeys and the other with five storeys. This project was accomplished jointly with CIMA+ and Honeywell and investments of 360,000 dollars and a guaranteed payback period of five years. After three years of follow-up, energy savings of 314,000 dollars were realized. The author declared that the project to date has been a success, and offered a few points to ponder in this document. The author argued that preparation is vital for the success of such an initiative. The first step involves a detailed energy audit in order to better determine priorities. Answers to several questions must also be obtained, such as the scope of the project, the allocated budget, amount of work required and time available in which to complete, etc. By contacting a specialized firm, it is important for the manager to get involved at all stages. If financial incentives are available from governments or other organizations, consider them as a bonus into your budget. Do not base the success of the entire project on these incentives. Finally, the author discussed the advantages to be derived from partnerships with the private sector in energy efficiency initiatives.

  9. Extended LaSalle's Invariance Principle for Full-Range Cellular Neural Networks

    Directory of Open Access Journals (Sweden)

    Mauro Di Marco

    2009-01-01

    Full Text Available In several relevant applications to the solution of signal processing tasks in real time, a cellular neural network (CNN is required to be convergent, that is, each solution should tend toward some equilibrium point. The paper develops a Lyapunov method, which is based on a generalized version of LaSalle's invariance principle, for studying convergence and stability of the differential inclusions modeling the dynamics of the full-range (FR model of CNNs. The applicability of the method is demonstrated by obtaining a rigorous proof of convergence for symmetric FR-CNNs. The proof, which is a direct consequence of the fact that a symmetric FR-CNN admits a strict Lyapunov function, is much more simple than the corresponding proof of convergence for symmetric standard CNNs.

  10. Efeitos fisiológicos da coffea arábica e coffea canephora

    OpenAIRE

    Rodrigues, Tânia Patrícia Barradas

    2014-01-01

    Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz O café é uma das bebidas mais consumidas no mundo, sendo ultrapassado apenas pela água. De entre as várias espécies de café identificadas, Coffea arabica L. (arabica) e Coffea canephora Pierre (robusta), representam a grande maioria do café consumido no mundo. Para além da espécie de café, o tipo de processamento a que os grãos são submetidos (via seca ou húmida), o grau de torra e o método...

  11. Identification of novel and conserved microRNAs in Coffea canephora and Coffea arabica

    Directory of Open Access Journals (Sweden)

    Guilherme Loss-Morais

    2014-12-01

    Full Text Available As microRNAs (miRNAs are important regulators of many biological processes, a series of small RNAomes from plants have been produced in the last decade. However, miRNA data from several groups of plants are still lacking, including some economically important crops. Here microRNAs from Coffea canephora leaves were profiled and 58 unique sequences belonging to 33 families were found, including two novel microRNAs that have never been described before in plants. Some of the microRNA sequences were also identified in Coffea arabica that, together with C. canephora, correspond to the two major sources of coffee production in the world. The targets of almost all miRNAs were also predicted on coffee expressed sequences. This is the first report of novel miRNAs in the genus Coffea, and also the first in the plant order Gentianales. The data obtained establishes the basis for the understanding of the complex miRNA-target network on those two important crops.

  12. SCALE-4 Analysis of LaSalle Unit 1 BWR Commercial Reactor Critical Configurations

    International Nuclear Information System (INIS)

    Gauld, I.C.

    2000-01-01

    Five commercial reactor criticals (CRCs) for the LaSalle Unit 1 boiling-water reactor have been analyzed using KENO V.a, the Monte Carlo criticality code of the SCALE 4 code system. The irradiated fuel assembly isotopics for the criticality analyses were provided by the Waste Package Design team at the Yucca Mountain Project in the US, who performed the depletion calculations using the SAS2H sequence of SCALE 4. The reactor critical measurements involved two beginning-of-cycle and three middle-of-cycle configurations. The CRCs involved relatively low-cycle burnups, and therefore contained a relatively high gadolinium poison content in the reactor assemblies. This report summarizes the data and methods used in analyzing the critical configurations and assesses the sensitivity of the results to some of the modeling approximations used to represent the gadolinium poison distribution within the assemblies. The KENO V.a calculations, performed using the SCALE 44GROUPNDF5 ENDF/B-V cross-section library, yield predicted k eff values within about 1% Δk/k relative to reactor measurements for the five CRCs using general 8-pin and 9-pin heterogeneous gadolinium poison pin assembly models

  13. Caffeine inheritance in interspecific hybrids of Coffea arabica x Coffea canephora (Gentianales, Rubiaceae

    Directory of Open Access Journals (Sweden)

    Regina H.G. Priolli

    2008-01-01

    Full Text Available Caffeine inheritance was investigated in F2 and BC1F1 generations between Coffea arabica var. Bourbon Vermelho (BV and Coffea canephora var. Robusta 4x (R4x. The caffeine content of seeds and leaves was determined during 2004 and 2005. Microsatellite loci-markers were used to deduce the meiotic pattern of chromosome pairing of tetraploid interspecific hybrids. Genetic analysis indicated that caffeine content in seeds was quantitatively inherited and controlled by genes with additive effects. The estimates of broad-sense heritability of caffeine content in seeds were high for both generations. In coffee leaves, the caffeine content (BSH from the same populations showed transgressive segregants with enhanced levels and high BSH. Segregation of loci-markers in BC1F1 populations showed that the ratios of the gametes genotype did not differ significantly from those expected assuming random associations and tetrasomic inheritance. The results confirm the existence of distinct mechanisms controlling the caffeine content in seeds and leaves, the gene exchange between the C. arabica BV and C. canephora R4x genomes and favorable conditions for improving caffeine content in this coffee population.

  14. The economic value of coffee (Coffea arabica) genetic resources

    NARCIS (Netherlands)

    Hein, L.G.; Gatzweiler, F.

    2006-01-01

    Whereas the economic value of genetic diversity is widely recognized there are, to date, relatively few experiences with the actual valuation of genetic resources. This paper presents an analysis of the economic value of Coffea arabica genetic resources contained in Ethiopian highland forests. The

  15. Analysis of the LaSalle Unit 2 Nuclear Power Plant: Risk Methods Integration and Evaluation Program (RMIEP)

    International Nuclear Information System (INIS)

    Payne, A.C. Jr.; Eide, S.A.; LaChance, J.C.; Whitehead, D.W.

    1992-10-01

    This volume presents the results of the initiating event and accident sequence delineation analyses of the LaSalle Unit II nuclear power plant performed as part of the Level III PRA being performed by Sandia National Laboratories for the Nuclear Regulatory Commission. The initiating event identification included a thorough review of extant data and a detailed plant specific search for special initiators. For the LaSalle analysis, the following initiating events were defined: eight general transients, ten special initiators, four LOCAs inside containment, one LOCA outside containment, and two interfacing LOCAs. Three accident sequence event trees were constructed: LOCA, transient, and ATWS. These trees were general in nature so that a tree represented all initiators of a particular type (i.e., the LOCA tree was constructed for evaluating small, medium, and large LOCAs simultaneously). The effects of the specific initiators on the systems and the different success criteria were handled by including the initiating events directly in the system fault trees. The accident sequence event trees were extended to include the evaluation of containment vulnerable sequences. These internal event accident sequence event trees were also used for the evaluation of the seismic, fire, and flood analyses

  16. Analysis of the LaSalle Unit 2 Nuclear Power Plant, Risk Methods Integration and Evaluation Program (RMIEP)

    International Nuclear Information System (INIS)

    Ferrell, W.L.; Payne, A.C. Jr.; Daniel, S.L.

    1992-10-01

    This report is a description of the internal flood analysis performed on the LaSalle County Nuclear Generating Station, Unit 2. A more detailed integration with the internal events analysis than in prior flood risk assessments was accomplished. The same system fault trees used for the internal events analysis were also used for the flood analysis, which included modeling of components down to the contact pair level. Subsidiary equations were created to map the effects of pipe failures. All component locations were traced and mapped into the fault trees. The effects of floods were then mapped directly onto the internal plant model and their relative importance was evaluated. A detailed screening analysis was performed which showed that most plant areas had a negligible contribution to the flood-induced core damage frequency. This was influenced strongly by the fact that the LaSalle plant was designed with a high level of concern about the effects of external events such as fire and flood and significant separation was maintained between systems in the original design. Detailed analysis of the remaining flood scenarios identified only two that contributed significantly to risk. The flood analysis resulted in a total (mean) core damage frequency of 3.23E-6 per year

  17. Oogenesis pattern and type of ovariole of the parasitoid Palmistichus elaeisis (Hymenoptera: Eulophidae

    Directory of Open Access Journals (Sweden)

    Gilberto S. Andrade

    2012-09-01

    Full Text Available The knowledge on ovigeny in parasitoids is important for basic studies on physiology and applied biological control. The ovigeny pattern and type of ovariole of the parasitoid Palmistichus elaeisis Delvare & LaSalle (Hymenoptera: Eulophidae were studied in newly-emerged females at seven, 14, 24 and 48 h intervals after their emergence from Tenebrio molitor L. pupae (Coleoptera: Tenebrionidae. Females of P. elaeisis presented ovaries composed by four ovarioles of the meroistic polytrophic type. The yolk accumulation and chorionogenesis in P. elaeisis were concluded 24 h after the female emergence. The 48 h-old females show a high quantity of egg ready for oviposition. These findings can help to improve the mass production of P. elaeisis and the augmentative biological control by using this natural enemy.O conhecimento da ovigenia em parasitóides é importante para estudos básicos em fisiologia e para o controle biológico aplicado. O padrão de ovigenia e OVIGENY OF Palmistichus elaeisis (HYMENOPTERA: EULOPHIDAE o tipo de ovaríolo do parasitóide Palmistichus elaeisis Delvare & LaSalle (Hymenopera: Eulophidae foram estudados em fêmeas recém-emergidas e em intervalos de sete, 14, 24 de 48 horas após a emergência em pupas de Tenebrio molitor L. (Coleoptera: Tenebrionidae. Fêmeas de P. elaesis apresentaram o ovário composto por quatro ovaríolos do tipo meroístico politrófico. A deposição de vitelo e corionogênese em P. elaeisis foram concluídas 24 horas após a emergência. Fêmeas com 48 horas de idade apresentam grande quantidade de ovos prontos para a oviposição. Esses resultados podem ajudar a melhoria da produção massal de P. elaeisis e o controle biológico aplicado com esse parasitóide.

  18. Analysis of the LaSalle Unit 2 nuclear power plant: Risk Methods Integration and Evaluation Program (RMIEP)

    International Nuclear Information System (INIS)

    Wells, J.E.; Lappa, D.A.; Bernreuter, D.L.; Chen, J.C.; Chuang, T.Y.; Johnson, J.J.; Campbell, R.D.; Hashimoto, P.S.; Maslenikov, O.R.; Tiong, L.W.; Ravindra, M.K.; Kincaid, R.H.; Sues, R.H.; Putcha, C.S.

    1993-11-01

    This report describes the methodology used and the results obtained from the application of a simplified seismic risk methodology to the LaSalle County Nuclear Generating Station Unit 2. This study is part of the Level I analysis being performed by the Risk Methods Integration and Evaluation Program (RMIEP). Using the RMIEP developed event and fault trees, the analysis resulted in a seismically induced core damage frequency point estimate of 6.OE-7/yr. This result, combined with the component importance analysis, indicated that system failures were dominated by random events. The dominant components included diesel generator failures (failure to swing, failure to start, failure to run after started), and condensate storage tank

  19. Antioxidant effect of Arabian coffee ( Coffea arabica L) blended with ...

    African Journals Online (AJOL)

    Purpose: To investigate the antioxidant activity of Coffea Arabica L in high-fat diet (HFD)-fed C57BL/6J mice. Methods: A decoction of Arabian coffee blended with or without cardamom or cloves was orally administered to HFD-fed C57BL/6J mice for a period of 60 days. At the end of the treatment, blood and tissue samples ...

  20. Palmistichus elaeisis (Hymenoptera: Eulophidae as an indicator of toxicity of herbicides registered for corn in Brazil

    Directory of Open Access Journals (Sweden)

    Claubert W.G de Menezes

    2014-09-01

    Full Text Available The diversity of plants in agricultural systems benefits natural enemies. Herbicides are used in weed management in corn (Zea mays L. to reduce competition and productivity losses, but they can impact natural enemies and contaminate the environment. The objective was to evaluate toxicity of herbicides on pupae parasitoid Palmistichus elaeisis Delvare and LaSalle, 1993 (Hymenoptera: Eulophidae. The treatments were represented by the host pupae Tenebrio molitor L., 1785 (Coleoptera: Tenebrionidae and herbicides atrazine, nicosulfuron, paraquat, and tembotrione in commercial doses compared to a control treatment with water. Pupae of T. molitor were immersed in the solution of herbicides and exposed to parasitism by six females of P. elaeisis each. The herbicides atrazine and paraquat were highly toxic and, therefore, not selective to P. elaeisis. Nicosulfuron reduced the sex ratio of P. elaeisis (0.20 ± 0.03, which may affect subsequent generations. Moreover, the herbicide tembotrione was selective to P. elaeisis, showing results comparable to the control. Floristic diversity of weeds can increase food source, habitat, shelter, breeding places and microclimates for insect parasitoids but herbicides formulations can be toxic and these products can affect P. elaeisis or its hosts by direct or indirect contact, showing the importance of selectivity studies for this natural enemy. However, the herbicide tembotrione was selective to P. elaeisis and it can be recommended for programs of sustainable management of weeds in corn crop with this parasitoid.

  1. Reproductive biology of Palmistichus elaeisis (Hymenoptera: Eulophidae with alternative and natural hosts

    Directory of Open Access Journals (Sweden)

    Fabricio F. Pereira

    2010-01-01

    Full Text Available Mass rearing of parasitoids depends on choosing appropriate alternative hosts. The objective of this study was to select alternative hosts to rear the parasitoid Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera: Eulophidae. Pupae of the lepidopterans Anticarsia gemmatalis Hübner, 1818 (Lepidoptera: Noctuidae, Bombyx mori Linnaeus, 1758 (Lepidoptera: Bombycidae and Thyrinteina arnobia (Stoll, 1782 (Lepidoptera: Geometridae were exposed to parasitism by females of P. elaeisis. The duration of the life cycle of P. elaeisis was 21.60 ± 0.16 and 24.15 ± 0.65 days on pupae of A. gemmatalis and B. mori, respectively, with 100.0% parasitism of the pupae and 71.4 and 100.0% emergence of parasitoids from the first and second hosts, respectively. The offspring number of P. elaeisis was 511.00 ± 49.70 and 110.20 ± 19.37 individuals per pupa of B. mori and A. gemmatalis, respectively. The reproduction of P. elaeisis from pupae of T. arnobia after six generations was similar to the other hosts.

  2. Quality of the surface of Coffea arabica wood

    Directory of Open Access Journals (Sweden)

    Pedro Paulo de Carvalho Braga

    2014-03-01

    Full Text Available The wood of Coffea arabica L. is considered a a residue of the coffee industry and is widely used as a source of energy. Few studies have shown other destinations such as the manufacture of small objects and furniture with rustic design. The objective of this work was to find the best fit in cutting speed during machining planer trowel the wood of Coffea arabica, taking into consideration the quality of the machined surface. The wood from the Coffea arabica came from an 15 years planting, spacing 3 x 2 m, of the municipality of Machado / MG. The tree was pruned, unfolded and flattened, in order for getting cut-proof of 30 mm thick, with variables length and width. The machining tests were performed at the Laboratory of Wood Machining (DCF / UFLA, varying the cutting speed in plane trowel. The qualification of the machined surface was performed by the feed per tooth (fz, visual analysis (ASTM D 1666-87 and roughness Ra and Rz. It was used a completely randomized design with 30 repetitions. We conducted the analysis of variance test and the average of Scott-Knott, at 5% significance level. It was calculated the percentage of marks obtained for the feed per tooth. The results showed that the quality of machined surface with cutting speeds of 19 and 21 m∙s-1 and forward speed of 6 m∙min-1 were satisfactory with small surveys of fiber and low values of feed per tooth ( fz and roughness Ra and Rz.

  3. PENGARUH PEMBERIAN LIMBAH KULIT KOPI (Coffea robusta L. TERHADAP PERTUMBUHAN CABAI KERITING (Capsicum annum L.

    Directory of Open Access Journals (Sweden)

    Zainal Berlian

    2015-08-01

    Full Text Available Curly chili (Capsicum annum L. is a kind of vegetable commodities which very demand among the Indonesian because of its spicy taste that can be used as a flavoring dishes and has a high economic value. The goal of this study is to determine the effect of bark compost coffee (Coffea robusta in growing media on the growth and development of plants curly chili (Capsicum annum L. and severe skin compost coffee (Coffea robusta which gives the maximum growth of the plant curly chili (Capsicum annum L.. This research is conducted in the Laboratory Science UIN Raden Fatah Palembang by using experimental methods and completely randomized design (CRD consisting of 4 treatments and 6 replications treatment namely: P0 = Without the addition of compost the coffee (Coffea robusta (control, P1= Adding compost the coffee (Coffea robusta 30 grams, P2= Adding compost the coffee (Coffea robusta 60 grams , P3 = Addition of compost skin (Coffea robusta coffee 90 grams. Data are analyzed by F test followed by a test BJND (Difference Distance Real Duncan. The parameters of this study are plant height, number of leaves (pieces, the amount of fruit, and fruit weight. The results show that the addition of compost the coffee (Coffea robusta 90 grams (treatment P3 gives a very real effect on the growth of plant height, number of leaves, number of fruits, and also fruit weight. The conclusion is compost the coffee (Coffea robusta gives effect to the addition of compost and bark coffee (Coffea robusta 90 grams provides maximum growth and development of the plant curly chili (Capsicum annum L..

  4. Study on the determination of coffea arabic radio sensibility curve

    International Nuclear Information System (INIS)

    Robleda, D.; Velazquez, R.

    1993-01-01

    The radiosensitivity curve of Coffea Arabic submitted to gamma irradiation from a 60C o source at the MRX-1500 with a dose power of 105 Gy/min was determined. For that study,. coffee seeds were irradiated at doses of 10,40,80 and 120 Gy, with a 40% of relative humidity; percentage of germination, height decrease, number and size of leaves, number and length of modes. As result of this analysis, GR50 and GR20 doses for searching genetic variability related to plant size reduction

  5. Quantification of Coffea arabica and Coffea canephora var. robusta concentration in blends by means of synchronous fluorescence and UV-Vis spectroscopies.

    Science.gov (United States)

    Dankowska, A; Domagała, A; Kowalewski, W

    2017-09-01

    The potential of fluorescence, UV-Vis spectroscopies as well as the low- and mid-level data fusion of both spectroscopies for the quantification of concentrations of roasted Coffea arabica and Coffea canephora var. robusta in coffee blends was investigated. Principal component analysis was used to reduce data multidimensionality. To calculate the level of undeclared addition, multiple linear regression (PCA-MLR) models were used with lowest root mean square error of calibration (RMSEC) of 3.6% and root mean square error of cross-validation (RMSECV) of 7.9%. LDA analysis was applied to fluorescence intensities and UV spectra of Coffea arabica, canephora samples, and their mixtures in order to examine classification ability. The best performance of PCA-LDA analysis was observed for data fusion of UV and fluorescence intensity measurements at wavelength interval of 60nm. LDA showed that data fusion can achieve over 96% of correct classifications (sensitivity) in the test set and 100% of correct classifications in the training set, with low-level data fusion. The corresponding results for individual spectroscopies ranged from 90% (UV-Vis spectroscopy) to 77% (synchronous fluorescence) in the test set, and from 93% to 97% in the training set. The results demonstrate that fluorescence, UV, and visible spectroscopies complement each other, giving a complementary effect for the quantification of roasted Coffea arabica and Coffea canephora var. robusta concentration in blends. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Diagnosis of Hymenoptera venom allergy

    NARCIS (Netherlands)

    Bilo, BM; Rueff, F; Mosbech, H; Bonifazi, F; Oude Elberink, JNG

    2005-01-01

    The purpose of diagnostic procedure is to classify a sting reaction by history, identify the underlying pathogenetic mechanism, and identify the offending insect. Diagnosis of Hymenoptera venom allergy thus forms the basis for the treatment. In the central and northern Europe vespid (mainly Vespula

  7. Sex determination in the Hymenoptera

    NARCIS (Netherlands)

    Heimpel, George E.; de Boer, Jetske G.

    2008-01-01

    The dominant and ancestral mode of sex determination in the Hymenoptera is arrhenotokous parthenogenesis, in which diploid females develop from fertilized eggs and haploid males develop from unfertilized eggs. We discuss recent progress in the understanding of the genetic and cytoplasmic mechanisms

  8. Enkele bijzondere bijenwaarnemingen (Hymenoptera: Apidae)

    NARCIS (Netherlands)

    Raemakers, I.P.

    2000-01-01

    Some interesting records on Dutch bees (Hymenoptera: Apidae) The publication of the preliminary atlas of Dutch bees (Peeters et al. 1999) has stimulated many specialists in their mapping activity. The author reports several interesting new distribution records on ten bee-species in 1999. The Dutch

  9. Influence of the genotype and density of inoculation on the differentiation of somatic embryos of Coffea arabica L. cv. Red Caturra and Coffea canephora cv. Robusta

    Directory of Open Access Journals (Sweden)

    Raúl Barbón

    2003-07-01

    Full Text Available The conditions were established for the differentiation of somatic embryos from cell suspensions in the genotype Caturra rojo (Coffea arabica and Robusta (Coffea canephora. Cell suspensions with high embryogenic potentials and stable coefficients of multiplication were used. While studying the density of inoculation, for the phase of differentiation for both varieties, differences appeared in the embryogenic capacity among them, being reached a whole of 556 500 ES.l-1 for the variety Caturra rojo and 298 670 SE.l-1 for the variety Robusta. The biggest number of embryos in torpedo state, were obtained with a density of inoculation of 0.5 gFW.l-1 for the variety Caturra rojo and 5.0 gMF.l-1 for the variety Robusta. Key Words: cell suspensions, embryogenic potential, somatic Embryogenesis, embryogenic cells

  10. O dimorfismo dos ramos em Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    A. Carvalho

    1950-06-01

    Full Text Available O dimorfismo dos ramos tem sido observado em vários gêneros de plantas, tais como Gossypium, Theobroma, Hedera, Musa, Araucária, Castilla, bem como em Coffea. Tal fenômeno se carateriza por uma diferenciação somática, que, na maioria dos casos, é permanente, podendo-se propagar as diferentes formas pela reprodução vegetativa. Como acontece nos representantes de Coffea, tal dimorfismo se carateriza por diferenças no hábito de crescimento, isto é, na direção dos ramos. Assim, a extremidade de um ramo ponteiro (ortotrópico reproduz, quando enxertada, uma planta normal, ao passo que a de um ramo lateral (plagiotrópico somente dará origem a ramos laterais. Em se tratando de um fenômeno tanto de interesse teórico como de importância prática (para a propagação vegetativa, resolveu-se fazer uma série de investigações em tôrno dêsse assunto, cujos primeiros resultados os autores relatam no presente trabalho. Apresentaram-se, em primeiro lugar, as observações feitas com referência à natureza das gemas existentes nas axilas das fôlhas de plantas novas, tanto na haste principal, como nos ramos laterais. Verificou-se que: a no eixo hipocotiledonar não há indícios da existência de gemas ; b na axila das fôlhas cotiledonares há um grupo de gemas dormentes, que são despertadas, dando origem a ramos ortotrópicos, quando o eixo epicotiledonar é cortado abaixo do primeiro par de fôlhas primárias; c o aparecimento de gemas, que dão ramos laterais (plagiotrópicos, só ocorre pela primeira vez, nas axilas do oitavo ao décimo primeiro par de folhas ; observou-se que certas estruturas genéticas impedem a formação de ramos plagiotrópicos mesmo até o trigésimo terceiro par de fôlhas ; d nas axilas da haste principal, possuidoras de gemas que dão origem a ramos plagiotrópicos, ocorrem também duas e, mais raramente, três outras, que produzem ramos ortotrópicos, e que se desenvolvem quando se suprime o eixo

  11. Pengaruh Bionematisida Berbahan Aktif Jamur Paecilomyces lilacinus Strain 251 terhadap Serangan Pratylenchus coffeae pada Kopi Robusta

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    Soekadar Wiryadiputra

    2002-07-01

    Full Text Available Experiment on the effect of P. lilacinus on the infestation of P. coffeae on robusta coffee was conducted in Sumber Asin Experimental Garden, Malang. The treatments were dosages of bionematicide i.e. : 0 (control, 0.25, 0.50, 1.00, 2.00, and 4.00 g; carbofuran (3 % active ingredient 50 g/plant and organic soil treatment (OST at 100 g/plant. Each treatment was replicated four times, and each replication consists of five coffee trees. The results in second year observation revealed that the population of P. coffeae in the roots on PL 251 treatments was not significantly different compared to the control, whereas in soil samples the population of both P. coffeae and Rotylenchulus reniformis inclined to be lower than the control, although they were not statistically significant. The lowest infestation was observed on PL 251 treatment at a dosage level of 4.00 g/tree. On nematode infestation, no significant difference on treatments of carbofuran and OST compared to the control. The yield of green coffee (market coffee was the highest on the treatment of PL 251 at a dosage of 4.00 g/tree and significantly higher than the control and carbofuran treatments, with increasing levels of 225.3 and 198.9%, respectively. Keywords: bionematicide Paecilomyces lilacinus strain 251 (PL 251, Pratylenchus coffeae, Rotylenchulus reniformis

  12. Genetic Diversity of the Invasive Gall Wasp Leptocybe invasa (Hymenoptera: Eulophidae and of its Rickettsia Endosymbiont, and Associated Sex-Ratio Differences.

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    Francesco Nugnes

    Full Text Available The blue-gum chalcid Leptocybe invasa Fisher & LaSalle (Hymenoptera: Eulophidae is a gall wasp pest of Eucalyptus species, likely native to Australia. Over the past 15 years it has invaded 39 countries on all continents where eucalypts are grown. The worldwide invasion of the blue gum chalcid was attributed to a single thelytokous morphospecies formally described in 2004. Subsequently, however, males have been recorded in several countries and the sex ratio of field populations has been found to be highly variable in different areas. In order to find an explanation for such sex ratio differences, populations of L. invasa from a broad geographical area were screened for the symbionts currently known as reproductive manipulators, and both wasps and symbionts were genetically characterized using multiple genes. Molecular analyses suggested that L. invasa is in fact a complex of two cryptic species involved in the rapid and efficient spread of the wasp, the first recovered from the Mediterranean region and South America, the latter from China. All screened specimens were infected by endosymbiotic bacteria belonging to the genus Rickettsia. Two closely related Rickettsia strains were found, each infecting one of the two putative cryptic species of L. invasa and associated with different average sex ratios. Rickettsia were found to be localized in the female reproductive tissues and transovarially transmitted, suggesting a possible role of Rickettsia as the causal agent of thelytokous parthenogenesis in L. invasa. Implications for the variation of sex ratio and for the management of L. invasa are discussed.

  13. Genetic Diversity of the Invasive Gall Wasp Leptocybe invasa (Hymenoptera: Eulophidae) and of its Rickettsia Endosymbiont, and Associated Sex-Ratio Differences

    Science.gov (United States)

    Nugnes, Francesco; Gebiola, Marco; Monti, Maurilia Maria; Gualtieri, Liberata; Giorgini, Massimo; Wang, Jianguo; Bernardo, Umberto

    2015-01-01

    The blue-gum chalcid Leptocybe invasa Fisher & LaSalle (Hymenoptera: Eulophidae) is a gall wasp pest of Eucalyptus species, likely native to Australia. Over the past 15 years it has invaded 39 countries on all continents where eucalypts are grown. The worldwide invasion of the blue gum chalcid was attributed to a single thelytokous morphospecies formally described in 2004. Subsequently, however, males have been recorded in several countries and the sex ratio of field populations has been found to be highly variable in different areas. In order to find an explanation for such sex ratio differences, populations of L. invasa from a broad geographical area were screened for the symbionts currently known as reproductive manipulators, and both wasps and symbionts were genetically characterized using multiple genes. Molecular analyses suggested that L. invasa is in fact a complex of two cryptic species involved in the rapid and efficient spread of the wasp, the first recovered from the Mediterranean region and South America, the latter from China. All screened specimens were infected by endosymbiotic bacteria belonging to the genus Rickettsia. Two closely related Rickettsia strains were found, each infecting one of the two putative cryptic species of L. invasa and associated with different average sex ratios. Rickettsia were found to be localized in the female reproductive tissues and transovarially transmitted, suggesting a possible role of Rickettsia as the causal agent of thelytokous parthenogenesis in L. invasa. Implications for the variation of sex ratio and for the management of L. invasa are discussed. PMID:25970681

  14. Genética de coffea VII: hereditariedade dos caracteres de coffea arabica L. var. maragogipe hort ex froehner

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    C. A. Krug

    1942-01-01

    Full Text Available A variedade maragogipe do Coffea arabica L. foi encontrada pela primeira vez por Crisógono José Fernandes, em 1870, no município baiano de Maragogipe onde, provavelmente, se originou por mutação. Desde 1933 esta variedade vem sendo estudada pela Secção de Genética do Instituto Agronômico do Estado de São Paulo, em Campinas, com o fim de se determinar a sua constituição genética. Muitas autofecundações, cruzamentos e back-crosses foram, então, realizados. Grande parte das plantas obtidas só puderam ser classificadas após a colheita do ano de 1940. Todas foram examinadas quanto à forma e dimensões das folhas e um grande número ainda quanto à forma e dimensões das flores, frutos e sementes. Verificou-se que o caráter maragogipe mostra dominância quase completa em F1, não sendo possivel uma separação das ciasses maragogipe puro e híbrido. Em F2, e nos back-crosses com as formas normais, obtiveram-se, respectivamente, relações de 3:1 e 1:1 entre plantas maragogipe e plantas normais, relações essas que demonstram que os caracteres do maragogipe são controlados por um único par de fatores genéticos dominantes, para os quais se propõe o símbolo Mg-Mg, derivado do próprio nome desta variedade.In the present article the results of the genetical analysis of the characters of the maragogipe variety of Coffea arabica L are presented. This variety which originated as a mutation from C. arabica L. var. typica Cramer, in 1870, in the State of Baía in North Brazil, represents a gigas form of that variety, having larger leaves, flowers and fruits, its plants being also taller; it is also known for its low productivity. Since 1933 a genetical analysis of this variety was undertaken, many of its plants being selfed and crossed with other maragogipe plants and also with individuals of the typica and bourbon varieties of C. arabica; two generations have been studied, including F2's and several back-crosses. It was concluded

  15. Parasitismo de Palmistichus elaeisis (Hymenoptera: Eulophidae em hospedeiro alternativo sobre plantas de eucalipto em semi-campo Parasitism of Palmistichus elaeisis (Hymenoptera: Eulophidae in alternative host on eucalypt in semi-field

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    Fabrício Fagundes Pereira

    2010-12-01

    Full Text Available O sucesso de programas de controle biológico com parasitóides depende de pesquisas aplicadas e, por isso, estimouse o número ideal de fêmeas de Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera: Eulophidae para liberação em plantios de eucalipto, visando o controle de lepidópteros desfolhadores. Em cada repetição foram utilizadas trinta e seis pupas do hospedeiro alternativo Anticarsia gemmatalis Hübner, 1818 (Lepidoptera: Noctuidae com 24 a 48 horas de idade que, foram individualizadas em armadilhas confeccionadas com tela de náilon e fixadas em ramos de plantas de Eucalyptus grandis nos terços superior, médio e inferior, na proporção de 33,33% (12 armadilhas. Após a fixação das armadilhas, as plantas foram cobertas individualmente, com uma gaiola de 7,0 x 7,0 x 2,5 m confeccionada com tecido organza e posteriormente liberadas, 36; 72; 144; 288; 576 ou 1.152 fêmeas de P. elaeisis, representando um, dois, quatro, oito, 16 ou 32 parasitóides por pupa e na testemunha, não houve liberação do parasitóide. Cada proporção foi considerada um tratamento, sendo instaladas seis repetições permitindo o parasitismo por 96 horas. O número de pupas de A. gemmatalis parasitadas foi crescente com o aumento do número de parasitóides liberados, independentemente do terço da planta considerado, ajustando-se a uma função quadrática com ponto de máximo próximo a 25 fêmeas por pupa. Na densidade de 32 fêmeas/pupa não houve aumento significativo no número de pupas parasitadas, sugerindo 25 fêmeas de P. elaeisis por pupa como a densidade mais próxima do ideal para liberação desse parasitóide em plantios de eucalipto.The success of programs of biological control with parasitoids depends on applied research and therefore it was estimated the optimal number of females Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera: Eulophidae for release in eucalyptus plantations, for the control of lepidopterans

  16. DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica

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    Meynarti Sari Dewi Ibrahim

    2013-10-01

    Full Text Available Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1. The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.

  17. Ploidy level and caffeine content in leaves of Coffea Ploidia e conteúdo de cafeína em folhas de Coffea

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    Maria Bernadete Silvarolla

    1999-07-01

    Full Text Available Several species of Coffea and varieties of C. arabica differing in chromosome number had their caffeine content determined in the leaves. For Coffea arabica var. "Angustifolia", var. "Caturra" and var. "Icatu", it was observed caffeine decrease from the haploid (2n = 22 to tetraploid leaves (2n = 44. Caffeine in the tetraploid "Angustifolia" was decreased by 50% when compared to the haploid. Caffeine reduction was also observed in leaves of C. canephora var. "Kouilou" as chromosome number was increased (2n = 22 ® 2n = 44. In this case, caffeine in the leaves of the diploid genotype was close to 4 times higher than in the tetraploid. On the other hand it was observed an increase of the alkaloid when the chromosome number was doubled in the C. canephora var. "Robusta".Variedades e espécies de café (Coffea que diferem quanto ao número de cromossomos tiveram o conteúdo de cafeína determinado em suas folhas. Para as variedades "Angustifolia", "Caturra" e "Icatu" de Coffea arabica foi observada redução no teor de cafeína de folhas haplóides (2n = 22 em relação a tetraplóides (2n = 44. Cafeína na forma tetraplóide da var. "Angustifolia" foi reduzida em 50% quando comparada com a forma haplóide. Redução de cafeína também foi observada em folhas de C. canephora var. "Kouilou" quando o número de cromossomos aumentou (2n = 22 ® 2n = 44. Neste caso, a cafeína nas folhas do genótipo diplóide foi cerca de quatro vezes maior que no tetraplóide. Por outro lado, foi observado aumento desse alcalóide quando o número de cromossomos foi duplicado em C. canephora var. "Robusta".

  18. Redistribution of the solar radiation and the rain inside of coffee plantations (Arabic Coffea L.)

    International Nuclear Information System (INIS)

    Jaramillo Robledo, Alvaro

    2005-01-01

    The following review presents a series of studies on microclimates of non-shaded and shaded conditions of coffee plantations (Coffea arabica L.) in Colombia. Likewise, The redistribution of solar radiation and the temperature, as well as the energy balance, of the coffee plant and the crop are described. The results on the components of water balance and transport of nutrients within the coffee plantations are reported

  19. Microbial inputs in coffee (Coffea arabica L.) production systems, southwestern Ethiopia

    OpenAIRE

    Muleta, Diriba

    2007-01-01

    Arabica coffee is the key cash crop and top mainstay of the Ethiopian economy and requires sustainable production methods. Southwestern natural forests, the site of this study, are believed to be the centre of origin and diversity for Coffea arabica and still harbour wild Arabica coffee that may serve as an important gene pool for future breeding. Cost reductions, sustainability and quality improvement are now the major priorities in coffee production systems and require organic growing of co...

  20. Hymenoptera stings in Brazil: a neglected health threat in Amazonas State

    OpenAIRE

    Costa, Allyson Guimarães; Chaves, Bárbara Aparecida; Murta, Felipe Leão Gomes; Sachett, Jacqueline Almeida Gonçalves; Sampaio, Vanderson Souza; Silva, Vanessa Costa; Monteiro, Wuelton Marcelo

    2018-01-01

    Abstract INTRODUCTION: Hymenoptera injuries are commonly caused by stinging insects. In Amazonas state, Brazil, there is no information regarding distribution, profile, and systemic manifestations associated with Hymenoptera injuries. METHODS: This study aimed to identify risk factors for systemic manifestation using the Brazilian Notifiable Diseases Surveillance System (2007 to 2015). RESULTS: Half of Hymenoptera injuries were caused by bee stings. Hymenoptera injuries were concentrate...

  1. Observações citológicas em Coffea: XV - Microsporogênese em Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    A. J. T. Mendes

    1950-03-01

    Full Text Available No presente trabalho são apresentadas as observações realizadas sôbre a microsporogênese nas variedades semperflorens e caturra, de Coffea arabica L. Notou-se que, no início da prófase, os cromossômios se colorem muito mal, não permitindo observações sôbre a sua morfologia; em paquitene, os cromossômios se apresentam com várias secções heteropicnóticas separadas por secções muito finas, que se colorem mal; o centrômero é bastante nítido e se acha ladeado de zonas bem heteropicnóticas; as extremidades dos braços dos cromossômios se colorem mal e se perdem no meio do citoplasma ; o nucléolo é bastante visível e a êle se acham ligados alguns cromossômios. É difícil determinar o número exato de cromossômios ligados ao nucléolo, tendo-se encontrado de 1 a 4. De paquitene a metáfase I, as fases se sucedem rapidamente. Em diplotene, os cromossômios são curtos, não mais se percebendo o centrômero. Em diaquinese os 22 pares de cromossômios se repelem pela sua parte mais colorida, onde se encontra o centrômero, e se unem pela parte clara, onde se notam os quiasmas ; o número de quiasmas, por célula, varia de 29 a 43 ; a média por bivalente é de 1,67, em semperjlorens, e 1,75, em caturra. Em metáfase I, o número médio de quiasmas, por bivalente,. é de 1,69, em semperjlorens, e 1,67, em caturra. Em anáfase I, os 22 pares de cromossômios se separam normalmente. Em telófase I, os cromossômios se colorem mal. Não há, praticamente, intercinese; os cromossômios contraem-se de novo e entram em anáfase II. A formação dos micrósporos é normal. Depois de soltos, ocorre a divisão nuclear, dando origem a dois núcleos com 22 cromossômios. Isto ocorre três a quatro dias antes da abertura das flores; o núcleo vegetativo é grande, esférico e homogêneo, colo-rindo-se mal; o núcleo reprodutivo é menor, reticulado, colore-se bem e se localiza na periferia da célula; ao seu redor se destaca uma por

  2. Hymenoptera Stings and the Acute Kidney Injury

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    Yashad Dongol

    2013-07-01

    Full Text Available Hymenoptera stings are a health concern. Apidae (bees, Vespidae (hornets, yellow jackets and wasps and Formicidae (ants are medically-important stinging insects under the order Hymenoptera. Clinical features from simple skin manifestations to severe and fatal organ injury are due to the hypersensitivity reactions and/ or the toxic effects of the venom inoculated. Here we discuss on Hymenoptera stings involving apids (honey bees and vespids (wasps, hornets and yellow jackets and their effect on renal function and associated morphological changes in the kidney. Despite the differences in venom composition and quantity released per sting in two insect groups, both lead to similar medical consequences, such as localised normal allergic reactions, mild to severe anaphylaxis and shock and multiple organ and tissue injury leading to multiple organ failure. Acute kidney injury (AKI is one of the unusual complications of Hymenoptera stings and has the basis of both immune-mediated and toxic effects. Evidence has proven that supportive therapy along with the standard medication is very efficient in completely restoring the kidney function without any recurrence.

  3. HYMENOPTERA ALLERGENS: FROM VENOM TO VENOME

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    Edzard eSpillner

    2014-02-01

    Full Text Available In Western Europe hymenoptera venom allergy primarily relates to venoms of the honeybee and the common yellow jacket. In contrast to other allergen sources, only a few major components of hymenoptera venoms had been characterized until recently. Improved expression systems and proteomic detection strategies have allowed the identification and characterization of a wide range of additional allergens. The field of hymenoptera venom allergy research has moved rapidly from focusing on venom extract and single major allergens to a molecular understanding of the entire venome as a system of unique and characteristic components. An increasing number of such components has been identified, characterized regarding function and assessed for allergenic potential. Moreover, advanced expression strategies for recombinant production of venom allergens allow selective modification of molecules and provide insight into different types of IgE reactivities and sensitization patterns. The obtained information contributes to an increased diagnostic precision in hymenoptera venom allergy and may serve for monitoring, reevaluation and improvement of current therapeutic strategies.

  4. De wespbijen (Nomada) van Nederland (Hymenoptera: Apidae)

    NARCIS (Netherlands)

    Smit, J.

    2004-01-01

    The wasp bees (Nomada) of the Netherlands (Hymenoptera: Apidae) In the Netherlands 44 species of Nomada are known to occur. The name wasp bee is derived from the often yellow and black colour, which give the insects a wasplike appearance. Nomada bees are cleptoparasites mostly with Andrena species.

  5. Basophil-activation tests in hymenoptera allergy

    NARCIS (Netherlands)

    Dubois, Anthony E. J.; van der Heide, Sicco

    The measurement of basophil-activation markers may be useful in detecting IgIE-mediated sensitization but the relevance for application of the basophil-activation test in prediction of clinical reactivity in Hymenoptera allergy is very limited. For this reason, this test currently has no established

  6. Genética de coffea VI: independência dos fatores xc xc (xanthocarpa e br br (bronze em coffea arabica L.

    Directory of Open Access Journals (Sweden)

    C. A. Krug

    1942-01-01

    Full Text Available Em artigos anteriores (1, 2 os autores demonstraram que a cor amarela dos frutos e a coloração bronzeada das folhas novas são, em Coffea arabica L, controladas, cada uma, por um único par de fatores genéticos (respectivamente xc xc e Br Br. Os híbridos F1 no primeiro caso com plantas de frutos vermelhos, e no segundo com plantas de folhas novas verdes, demonstraram tratar-se de casos em que há dominância incompleta nesta geração, os frutos híbridos possuindo uma coloração vermelho clara e as folhas novas se apresentando com uma tonalidade bronze clara. Como algumas das hibridações realizadas envolviam, ao mesmo tempo, os dois caracteres em questão, apresentou-se a oportunidade para constatar se havia ou não independência entre os dois pares de fatores que controlam estes caracteres. Neste artigo apresentam-se os resultados das observações realizadas, tanto em diversas populações de F2 como também em dois back-crosses. Os dados confirmam plenamente a hipótese estabelecida, isto é, da independência entre os dois pares de fatores em questão (xc xc e Br Br. Este fato era esperado à vista do número relativamente elevado de cromosômios nas variedades cruzadas (2n = 44.In two previous publications (1,2 the authors demonstrated that in Coffea arabica L. yellow fruit color and bronze color of young leaves are each controled by one pair of genes (respectively xc xc and Br Br. The F1 hybrids, in the first instance between plants with yellow and red fruits and in the second instance between plants with bronze and green colored young leaves, showed incomplete dominance of both characters, the F1 fruits being of a light red color and the F1 young leaves of a light bronze one. As some of the crosses involved both pairs of genes, it was possible to find out wether they are linked or independent. In the present article the authors are publishing the results obtained with several F2 populations and also with 2 types of back crosses

  7. Single locus complementary sex determination in Hymenoptera : an "unintelligent" design?

    NARCIS (Netherlands)

    Wilgenburg, Ellen van; Driessen, Gerard; Beukeboom, Leo W.

    2006-01-01

    The haplodiploid sex determining mechanism in Hymenoptera (males are haploid, females are diploid) has played an important role in the evolution of this insect order. In Hymenoptera sex is usually determined by a single locus, heterozygotes are female and hemizygotes are male. Under inbreeding,

  8. Single locus complementary sex determination in Hymenoptera: an "unintelligent" design?

    NARCIS (Netherlands)

    van Wilgenburg, E.; Driessen, G.J.J.; Beukeboom, L.W.

    2006-01-01

    The haplodiploid sex determining mechanism in Hymenoptera (males are haploid, females are diploid) has played an important role in the evolution of this insect order. In Hymenoptera sex is usually determined by a single locus, heterozygotes are female and hemizygotes are male. Under inbreeding,

  9. Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation

    OpenAIRE

    Ribas, Alessandra Ferreira; Kobayashi, Adilson Kenji; Pereira, Luiz Filipe Protasio; Vieira, Luiz Gonzaga Esteves

    2006-01-01

    Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supp...

  10. Productivity of coffee crop (Coffea arabica L.) in conversion to the organic production system

    OpenAIRE

    Malta, Marcelo Ribeiro; Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG; Pereira, Rosemary Gualberto Fonseca Alvarenga; Universidade Federal de Lavras - UFLA; Chagas, Sílvio Júlio de Rezende; Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG; Guimarães, Rubens José; Universidade Federal de Lavras - UFLA

    2008-01-01

    This experiment was carried out in Lavras, MG, to verify the productivity of coffee crop (Coffea arabica L.) in conversion to the organic production system. The experiment was set in a six-year old coffee crop of the cultivar Catuaí Amarelo IAC 86, with spacing of 4,0 x 0,6 m, previously cultivated under the conventional system. In the organic treatments a 4 x 4 balanced lattice design with 5 replications in a 3 x 2 x 2 factorial scheme was used, besides 4 additional treatments. The f...

  11. The gall wasp Leptocybe invasa (Hymenoptera: Eulophidae) stimulates different chemical and phytohormone responses in two Eucalyptus varieties that vary in susceptibility to galling.

    Science.gov (United States)

    Li, X Q; Liu, Y Z; Guo, W F; Solanki, M K; Yang, Z D; Xiang, Y; Ma, Z C; Wen, Y G

    2017-09-01

    Gall-inducing insects produce various types of galls on plants, but little is known about the gall-induction mechanism of these galling insects. The gall wasp Leptocybe invasa Fisher & LaSalle (Hymenoptera: Eulophidae) forms galls of different sizes on several Eucalyptus species. To clarify the physiological responses of Eucalyptus to L. invasa infestation, we measured the dynamics of nitrogen (N), carbon (C), total phenolics, total tannins and four types of phytohormones (zeatin [Z] + zeatin riboside [ZR], gibberellins [GA], indole-3-acetic acid [IAA] and abscisic acid [ABA]) in galled and ungalled leaf tissues of two Eucalyptus horticultural varieties (DH201-2 [Eucalyptus grandis × Eucalyptus camaldulensis] and EA [Eucalyptus exserta]) with different susceptibility to galling throughout the larval developmental stages. Nitrogen, total phenolics, tannins and four kinds of phytohormones strongly accumulated in tissues galled by L. invasa (especially during early larval feeding stages). While N, Z + ZR and GA levels were higher, tannins and ABA levels were lower in the galled tissues on the highly susceptible variety. Nitrogen, total phenolics, GA, Z + ZR and IAA levels in the galled tissues gradually decreased during gall development, but ABA and tannins conversely increased in the galled tissues of the less susceptible variety. Our results suggest that the effects of gall-inducing insects on plants depend not only on the susceptibility of the plant infested but also on the developmental stage of galled tissues. Gall formation process is thus synergistically influenced by both gall-inducing insect and plant genotypes. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  12. Exogenous gibberellins inhibit coffee (Coffea arabica cv. Rubi) seed germination and cause cell death in the embryo

    NARCIS (Netherlands)

    Silva, Da E.A.A.; Toorop, P.E.; Nijsse, J.; Bewley, J.D.; Hilhorst, H.W.M.

    2005-01-01

    The mechanism of inhibition of coffee (Coffea arabica cv. Rubi) seed germination by exogenous gibberellins (GAs) and the requirement of germination for endogenous GA were studied. Exogenous GA4+7 inhibited coffee seed germination. The response to GA4+7 showed two sensitivity thresholds: a lower one

  13. Impacts of leaf age and heat stress duration on photosynthetic gas exchange and foliar nonstructural carbohydrates in Coffea arabica

    Science.gov (United States)

    Danielle E. Marias; Frederick C. Meinzer; Christopher Still

    2017-01-01

    Given future climate predictions of increased temperature, and frequency and intensity of heat waves in the tropics, suitable habitat to grow ecologically, economically, and socially valuable Coffea arabica is severely threatened. We investigated how leaf age and heat stress duration impact recovery from heat stress in C. arabica...

  14. Inhibitory effect of Coffea arabica bean in testosterone induced prostatic hyperplasia in Sprague-Dawley rats

    Directory of Open Access Journals (Sweden)

    Kristian Alfonso G. Cueto

    2016-05-01

    Full Text Available Benign prostatic hyperplasia (BPH has been described as the uncontrolled prostate gland growth which leads to difficulty in urination. One of the treatment of BPH is saw palmetto lipid extracts which has been shown to inhibit prostate 5 α-reductase and some of its components (lauric acid, myristic acid and oleic acid also inhibit the enzyme. Coffee was also rich in fatty acids namely linoleic acid, oleic acid and palmitic acid. The aim of this research is to investigate whether coffee is effective in preventing testosterone-induced prostatic hyperplasia in rats using testosterone propionate and estradiol valerate. After and before the induction, the rats were tested for prostate specific antigen (PSA . The condition of the prostate gland of the test animals were correlated with the results of the said test and in the histopathologic results. After 14 days of experimentation, animals in the test group significantly decreased their PSA levels as compared to the BPH group. The histomorphology showed that Coffea arabica bean oil inhibited testosterone propionate while estradiol valerate induced prostatic hyperplasia. These findings indicate that Coffee arabica bean oil effectively inhibited the development of BPH. With the proven safety of coffee oil, these findings strongly support the feasibility of using Coffea arabica bean oil therapeutically in treating BPH.

  15. Identification and chromosomal distribution of copia-like retrotransposon sequences in the coffee (Coffea L. genome

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    Juan-Carlos Herrera

    2013-12-01

    Full Text Available The presence of copia-like transposable elements in seven coffee (Coffea sp. species, including the cultivated Coffea arabica, was investigated. The highly conserved domains of the reverse transcriptase (RT present in the copia retrotransposons were amplified by PCR using degenerated primers. Fragments of roughly 300 bp were obtained and the nucleotide sequence was determined for 36 clones, 19 of which showed good quality. The deduced amino acid sequences were compared by multiple alignment analysis. The data suggested two distinct coffee RT groups, designated as CRTG1 and CRTG2. The sequence identities among the groups ranged from 52 to 60% for CRTG1 and 74 to 85% for CRTG2. The multiple alignment analysis revealed that some of the clones in CRTG1 were closely related to the representative elements present in other plant species such as Brassica napus, Populus ciliata and Picea abis. Furthermore, the chromosomal localization of the RT domains in C. arabica and their putative ancestors was investigated by fluorescence in situ hybridization (FISH analysis. FISH signals were observed throughout the chromosomes following a similar dispersed pattern with some localized regions exhibiting higher concentrations of those elements, providing new evidence of their relative conservation and stability in the coffee genome

  16. Evaluation of Somatic Embryogenesis Ability in Robusta Coffee (Coffea canephora Pierre

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    Priyono Priyono

    2010-08-01

    Full Text Available Embriogenesis somatik diharapkan sebagai metode perbanyakan tanaman yang sangat efektif pada kopi. Evaluasi dua jenis proses embriogenesis somatik, yaitu proses langsung dan tidak langsung akan bermanfaat untuk menggambarkan kemampuan proliferasi sel. Penelitian untuk mengevaluasi embriogenesis somatik kopi Robusta (Coffea canephora yang mempunyai tingkat keragaman genetik tinggi telah dilakukan di Nestlé R&D Centre Tours, Perancis. Bahan tanam menggunakan kopi Robusta koleksi Nestle Perancis dan tiga klon koleksi Pusat Penelitian Kopi dan Kakao Indonesia (Puslitkoka. Tiga aspek, yaitu proses embriogenesis, keragaman embriogenesis dan kemantapan embriogenesis dievaluasi dalam penelitian ini. Hasil penelitian menunjukkan bahwa baik embriogenesis somatik langsung maupun tidak langsung dapat diamati. Penelitian ini menunjukkan bahwa kedua proses embriogenesis somatik tersebut merupakan dua mekanisme yang berbeda. Dalam penelitian ini ditunjukkan bahwa kemampuan embriognesis somatik tergantung pada genotipe, baik antar maupun di dalam kelompok genetik kopi Robusta, yaitu Congolese,Guinean dan Conillon. Lebih lanjut diketahui bahwa kedua proses embriogenesis somatik tersebut stabil terhadap indukan sebagai sumber eksplan. Kemampuan embriogenesis somatik tidak langsung ketiga klon Puslitkoka (BP409, BP961 dan Q121 sangat beragam, sehingga memberikan harapan adanya pola segregasi yang baik berdasarkan kemampuan embriogenesis somatik tidak langsung pada populasi yang dibuat dari silangan klon tersebut.Key words: Coffea canephora, somatic embryogenesis, variability, stability, genotype.

  17. Anatomical and chemical properties and density of Coffea arabica L. wood

    Directory of Open Access Journals (Sweden)

    Marisa Aparecida Pereira

    2014-09-01

    Full Text Available The state of Minas Gerais is the largest producer of coffee in Brazil and the amount of residue in crops seems adequate to support production of solid wood products of Coffea arabica L., which is currently used for energy purposes or remains in the area. This activity adds insignificant value the coffee products and release CO2, which has harmful effects to the environment. This study was conducted with the aim of characterizing technologically Coffea arabica L. wood to enhance its use in furniture, to characterize its anatomical, chemical and wood basic density. The density showed an average of 0.608g.cm-3. The anatomical analysis showed distinct growth layers, semiporosos vessels with simple perforation plates. The axial parenchyma is apotracheal and diffuse in the aggregate with heterogeneous rays, not laminated and fiber libriformes not septate with bordered pits distinct. The chemical content of extract in hot and cold water were respectively 6.1% and 9.6%. The ash content was found to be 0.68%. Data were comparable to those of mahogany (Swietenia macrophylla and Piptadenia peregrina Benth, (angico-vermelho used for the production of furniture.

  18. Komunitas Nematoda pada Tanaman Kopi (Coffea Canephora Var. Robusta Muda di Kabupaten Tanggamus Lampung

    Directory of Open Access Journals (Sweden)

    I GEDE SWIBAWA

    2015-09-01

    Full Text Available The Community of Nematode in The Young Coffee ( Coffea Canephora Var. Robusta Crops in Tanggamus District, Lampung. Tanggamus district is one of coffee production center in Lampung province. Since year of 2013, farmers in Tanggamus have been replaced the unproductive old coffee by coffee seed introduced from East Java. Introducing coffe seed from outside area at risk of carrying plant parasitic nematodes. The purpose of this research was to study community of nematode associated with young coffee crops in Tanggamus. Survey was conducted in coffee robusta (Coffea canephora var. robusta fields belonging to farmer on September 2014. Soil samples were collected from three sites: Margo Mulyo, Sumber Rejo and Batu Bedil. Nematodes were extracted by sieving and centrifugation with sugar solution method. The results show that were 20 genera consisted of 9 genera of plant parasitic and 11 genera of free living nematodes associated with young coffee in Tanggamus. The nematode community was dominated by Pratylenchus and Radopholus. The population of Pratylenchus and Radopholus in Sumber Rejo site were 421 and 846 individual per 300 ml of soil respectively. It was needed to indentify up to species taxonomic level for Pratylenchus and Radopholus associated with young coffee in Tanggamus.

  19. Component Resolved Diagnosis in Hymenoptera Anaphylaxis.

    Science.gov (United States)

    Tomsitz, D; Brockow, K

    2017-06-01

    Hymenoptera anaphylaxis is one of the leading causes of severe allergic reactions and can be fatal. Venom-specific immunotherapy (VIT) can prevent a life-threatening reaction; however, confirmation of an allergy to a Hymenoptera venom is a prerequisite before starting such a treatment. Component resolved diagnostics (CRD) have helped to better identify the responsible allergen. Many new insect venom allergens have been identified within the last few years. Commercially available recombinant allergens offer new diagnostic tools for detecting sensitivity to insect venoms. Additional added sensitivity to nearly 95% was introduced by spiking yellow jacket venom (YJV) extract with Ves v 5. The further value of CRD for sensitivity in YJV and honey bee venom (HBV) allergy is more controversially discussed. Recombinant allergens devoid of cross-reactive carbohydrate determinants often help to identify the culprit venom in patients with double sensitivity to YJV and HBV. CRD identified a group of patients with predominant Api m 10 sensitization, which may be less well protected by VIT, as some treatment extracts are lacking this allergen. The diagnostic gap of previously undetected Hymenoptera allergy has been decreased via production of recombinant allergens. Knowledge of analogies in interspecies proteins and cross-reactive carbohydrate determinants is necessary to distinguish relevant from irrelevant sensitizations.

  20. Hymenoptera venom review focusing on Apis mellifera

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    P. R. de Lima

    2003-01-01

    Full Text Available Hymenoptera venoms are complex mixtures containing simple organic molecules, proteins, peptides, and other bioactive elements. Several of these components have been isolated and characterized, and their primary structures determined by biochemical techniques. These compounds are responsible for many toxic or allergic reactions in different organisms, such as local pain, inflammation, itching, irritation, and moderate or severe allergic reactions. The most extensively characterized Hymenoptera venoms are bee venoms, mainly from the Apis genus and also from social wasps and ant species. However, there is little information about other Hymenoptera groups. The Apis venom presents high molecular weight molecules - enzymes with a molecular weight higher than 10.0 kDa - and peptides. The best studied enzymes are phospholipase A2, responsible for cleaving the membrane phospholipids, hyaluronidase, which degrades the matrix component hyaluronic acid into non-viscous segments and acid phosphatase acting on organic phosphates. The main peptide compounds of bee venom are lytic peptide melittin, apamin (neurotoxic, and mastocyte degranulating peptide (MCD.

  1. Diversity of peptidic and proteinaceous toxins from social Hymenoptera venoms.

    Science.gov (United States)

    Dos Santos-Pinto, José Roberto Aparecido; Perez-Riverol, Amilcar; Lasa, Alexis Musacchio; Palma, Mario Sergio

    2018-06-15

    Among venomous animals, Hymenoptera have been suggested as a rich source of natural toxins. Due to their broad ecological diversity, venom from Hymenoptera insects (bees, wasps and ants) have evolved differentially thus widening the types and biological functions of their components. To date, insect toxinology analysis have scarcely uncovered the complex composition of bee, wasp and ant venoms which include low molecular weight compounds, highly abundant peptides and proteins, including several allergens. In Hymenoptera, these complex mixtures of toxins represent a potent arsenal of biological weapons that are used for self-defense, to repel intruders and to capture prey. Consequently, Hymenoptera venom components have a broad range of pharmacological targets and have been extensively studied, as promising sources of new drugs and biopesticides. In addition, the identification and molecular characterization of Hymenoptera venom allergens have allowed for the rational design of component-resolved diagnosis of allergy, finally improving the outcome of venom immunotherapy (VIT). Until recently, a limited number of Hymenoptera venoms had been unveiled due to the technical limitations of the approaches used to date. Nevertheless, the application of novel techniques with high dynamic range has significantly increased the number of identified peptidic and proteinaceous toxins. Considering this, the present review summarizes the current knowledge about the most representative Hymenoptera venom peptides and proteins which are under study for a better understanding of the insect-caused envenoming process and the development of new drugs and biopesticides. Copyright © 2018 Elsevier Ltd. All rights reserved.

  2. Mieren in Veluwebermen: soortenrijkdom en aanbevelingen voor beheer (Hymenoptera: Formicidae).

    NARCIS (Netherlands)

    Noordijk, J.; Boer, P.

    2007-01-01

    Ants in roadside verges on the Veluwe: species richness and recommendations for management (Hymenoptera: Formicidae) Highway verges in the Veluwe region contain some well developed nutrient poor plant communities, like grasslands, grey hair grass vegetation and heather vegetation. These places

  3. New faunistic data on the family Braconidae (Hymenoptera from Korea

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    Tae-Ho An

    2014-12-01

    Full Text Available Twenty-one species of the four genera in the family Braconidae (Hymenoptera are reported for the first time in Korea. These species belong to the subfamily Euphorinae (Braconidae. Faunistic and distribution data are included.

  4. Identification and Analysis of Jasmonate Pathway Genes in Coffea canephora (Robusta Coffee) by In Silico Approach.

    Science.gov (United States)

    Bharathi, Kosaraju; Sreenath, H L

    2017-07-01

    Coffea canephora is the commonly cultivated coffee species in the world along with Coffea arabica . Different pests and pathogens affect the production and quality of the coffee. Jasmonic acid (JA) is a plant hormone which plays an important role in plants growth, development, and defense mechanisms, particularly against insect pests. The key enzymes involved in the production of JA are lipoxygenase, allene oxide synthase, allene oxide cyclase, and 12-oxo-phytodienoic reductase. There is no report on the genes involved in JA pathway in coffee plants. We made an attempt to identify and analyze the genes coding for these enzymes in C. canephora . First, protein sequences of jasmonate pathway genes from model plant Arabidopsis thaliana were identified in the National Center for Biotechnology Information (NCBI) database. These protein sequences were used to search the web-based database Coffee Genome Hub to identify homologous protein sequences in C. canephora genome using Basic Local Alignment Search Tool (BLAST). Homologous protein sequences for key genes were identified in the C. canephora genome database. Protein sequences of the top matches were in turn used to search in NCBI database using BLAST tool to confirm the identity of the selected proteins and to identify closely related genes in species. The protein sequences from C. canephora database and the top matches in NCBI were aligned, and phylogenetic trees were constructed using MEGA6 software and identified the genetic distance of the respective genes. The study identified the four key genes of JA pathway in C. canephora , confirming the conserved nature of the pathway in coffee. The study expected to be useful to further explore the defense mechanisms of coffee plants. JA is a plant hormone that plays an important role in plant defense against insect pests. Genes coding for the 4 key enzymes involved in the production of JA viz., LOX, AOS, AOC, and OPR are identified in C. canephora (robusta coffee) by

  5. Sensorial analysis of irradiated coffee (Coffea arabica L.) by electron beam

    International Nuclear Information System (INIS)

    Rodrigues, Flavio T.; Fanaro, Gustavo B.; Koike, Amanda C.R.; Villavicencio, Anna Lucia C.H.; Silva, Maria E.M. Pinto e

    2013-01-01

    Coffee is an important commodity and it is one of the most widely consumed beverages in the world. The acceptance of coffee by consumers depends mainly on the sensory characteristics of the beverage, that is its flavor, body, color, acidity and aroma. Food irradiation is processing technology environmental friendly and safety which aimed at the improvement of food quality. Depending on the absorbed radiation dose various effects can be achieved resulting in increase the shelf life, disinfestation, microorganism load reduction, without causing sensory changes to the food. Sensory analysis is the examination of a food through the evaluation of the attributes sensorial of product. The objective this paper was to evaluate the sensory properties, acceptance and purchase intent by the consumer of coffee (Coffea arabica L.) after the irradiation process with doses 6.0, 12.0 and 18.0kGy by electron beam. (author)

  6. Estudo cariométrico dos poliplóides de Coffea

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    Giorgio Schreiber

    1946-01-01

    Full Text Available Foi estudado o volume nuclear de uma série de plantas de café com 22-44-66-88 cromossômios: C. congensis, C. arabica var. monosperma, C. arabica var. typica e C. arabica var. bullata e pelo método estatístico cariométrico. Das curvas de frequência (que não são "normais", foi calculado o valor normal (modo para cada número de cromossômios. Com êstes valores modais e os respectivos números de cromossômios foi calculada a equação de regressão, o êrro de estimação e o coeficiente de correlação. A correlação é perfeita, sendo, porém, a espécie C. congensis discordante dos demais elementos da série por ter um valor volumétrico correspondente a 33 cromossômios apesar dos 22 encontrados nas metáfases somáticas. Êste resultado é apontado para ulteriores investigações citogenéticas sôbre esta espécie. O resultado geral destas pesquisas mostra a perfeita correlação entre o número de genomas haplóides e o volume nuclear ; deve ser considerado, porém, válido somente no confronto entre núcleos que se encontram na mesma fase do ciclo de crescimento interfásico.Caryometric researches on Coffea polyploids. General discussion of the problem and preliminary results. A series of polyploid plant of Coffea with 22, 44, 66, and 88 chromosomes (C. congensis, C. arabica var. monosperma, C. arabica var. typica, C. arabica var. bullata have been studied with caryometric statistical method. The chromosome numbers have been plotted again,st the modal value of the frequency curves of nuclear volume and the regression equation, standard error of estimation and correlation coefficent have been calculated. The correlation results perfect between the chromosome number and the nuclear volume for all the elements of the polyploid series of Coffea arabica. Coffea congensis, althought with 22 chromosomes, has a nuclear volume exactly correspondent to that of C. arabica with 33 chromosomes. This fact is appointed for further

  7. Sensorial analysis of irradiated coffee (Coffea arabica L.) by electron beam

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Flavio T.; Fanaro, Gustavo B.; Koike, Amanda C.R.; Villavicencio, Anna Lucia C.H., E-mail: flaviot@ymail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Silva, Maria E.M. Pinto e [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Fac. de Saude Publica. Dept. de Nutricao

    2013-07-01

    Coffee is an important commodity and it is one of the most widely consumed beverages in the world. The acceptance of coffee by consumers depends mainly on the sensory characteristics of the beverage, that is its flavor, body, color, acidity and aroma. Food irradiation is processing technology environmental friendly and safety which aimed at the improvement of food quality. Depending on the absorbed radiation dose various effects can be achieved resulting in increase the shelf life, disinfestation, microorganism load reduction, without causing sensory changes to the food. Sensory analysis is the examination of a food through the evaluation of the attributes sensorial of product. The objective this paper was to evaluate the sensory properties, acceptance and purchase intent by the consumer of coffee (Coffea arabica L.) after the irradiation process with doses 6.0, 12.0 and 18.0kGy by electron beam. (author)

  8. A checklist of Ropalidiini wasps (Hymenoptera: Vespidae: Polistinae in Indochina

    Directory of Open Access Journals (Sweden)

    Pham Phong Huy

    2014-01-01

    Full Text Available As a basis for intensive study of the taxonomy and biogeography of Ropalidiini wasps in Indochina (Hymenoptera: Vespidae: Polistinae, a checklist of Ropalidiini wasps (Hymenoptera: Vespidae is presented. A total of 57 Ropalidiini species and subspecies belonging to three genera from Indochina are listed, together with information of the type material deposited in the Natural History Collection, Ibaraki University, Japan (IUNH and the Institute of Ecology and Biological Resources (IEBR. References of their distribution in Indochina are also provided.

  9. Effect of the inoculation density in Coffea arabica L. cv. `Caturra rojo' somatic embryos germination in RITA® Temporary Immersion System

    Directory of Open Access Journals (Sweden)

    Raúl Barbon

    2014-04-01

    Full Text Available The development of somatic embryogenesis of coffee (Coffea spp. in liquid culture medium is a viable alternative for the propagation of these species. The use of liquid culture medium and temporary immersion systems could increase the germination of somatic embryos and improve the quality of plants. The objective of this work was to determine the effect of inoculation density on germination of somatic embryos of Coffea arabica L. cv. `Caturra rojo' in temporary immersion systems RITA®. It were used as inoculum densities 40, 50, 60, 70 and 80 somatic embryos per RITA®. After 90 days of culture the number of somatic embryos germinated, hyperhydricity symptoms, number of true leaves, length and root development was quantified. With inoculum density of 70 somatic embryos per RITA®, it was obtained a highest germination percentage (60% with good leaf development and length of the plants. Key words: hyperhydricity, liquid culture medium, partial germination, total germination, somatic embryogenesis

  10. Natural history of interaction between Meteorus sp. Haliday, 1835 (Hymenoptera: Braconidae) and its hyperparasitoid Toxeumella albipes Girault, 1913 (Hymenoptera: Pteromalidae).

    Science.gov (United States)

    Sobczak, J F; Maia, D P; Moura, J C M S; Costa, V A; Vasconcellos-Neto, J

    2012-02-01

    Some parasitoids build a cocoon mass that hangs in the host body until the adults emergence, which is an advantage against attack by predators who troll the vegetation in search of prey. However, such behaviour is not effective against the hyperparasitoid attacks. This study reports the interaction between the caterpillar Manduca sexta Linnaeus, 1763 (Lepidoptera, Sphingidae) parasitised by Meteorus sp. (Hymenoptera, Braconidae) larvae and its hyperparasitoid Toxeumella albipes (Hymenoptera, Pteromalidae). This is the first description of the attack and oviposition of T. albipes.

  11. Revealing the Diversity of Introduced Coffea canephora Germplasm in Ecuador: Towards a National Strategy to Improve Robusta

    OpenAIRE

    Loor Solórzano, Rey Gastón; De Bellis, Fabien; Leroy, Thierry; Plaza, Luis; Guerrero, Hilton; Subia, Cristian; Calderón, Darío; Fernández, Fabián; Garzón, Iván; Lopez, Diana; Vera, Danilo

    2017-01-01

    Genetic resources of Coffea canephora have been introduced in several tropical countries with potential for crop development. In Ecuador, the species has been cultivated since the mid-20th century. However, little is known about the diversity and genetic structure of introduced germplasm. This paper provides an overview of the genetic and phenotypic diversity of C. canephora in Ecuador and some proposals for implementing a breeding program. Twelve SSR markers were used to analyze 1491 plants ...

  12. Identification by the DArTseq method of the genetic origin of the #Coffea canephora# cultivated in Vietnam and Mexico

    OpenAIRE

    Garavito, Andrea; Montagnon, Christophe; Guyot, Romain; Bertrand, Benoît

    2016-01-01

    Background The coffee species Coffea canephora is commercially identified as ?Conilon? when produced in Brazil, or ?Robusta? when produced elsewhere in the world. It represents approximately 40?% of coffee production worldwide. While the genetic diversity of wild C. canephora has been well studied in the past, only few studies have addressed the genetic diversity of currently cultivated varieties around the globe. Vietnam is the largest Robusta producer in the world, while Mexico is the only ...

  13. Effects of gamma radiation of Cobalt-60 on arabica and conillon seeds coffea: physic-chemistry evaluation

    International Nuclear Information System (INIS)

    Silva, Marcus Henriques da

    2012-01-01

    Brazil is the largest producer and exporter of coffee in the world. The coffee bean is one of the main products of the Brazilian trade balance. Two species of coffee are the most economically important: the Coffea arabica L. and Coffea canephora Pierre is the largest representative of the Coffea canephora Pierre is the coffea conillon. Food irradiation is an area of research that aims to increase the shelf life of foods and controlling pests. This study aimed to verify the physicochemical variables of Arabica coffee and conillon were affected when exposed to doses of gamma radiation from cobalt-60. The samples were provided by Polo in Coffee Quality Technology, Federal University of Lavras - UFLA. The coffee samples were subjected to irradiation doses: 0 (control), 5 kGy and 10 kGy, a multipurpose irradiator of IPEN - Research Institute of Nuclear Energy and the University of São Paulo, at a rate of 7.5 kGy / hour. For irradiation the samples were vacuum-packed in appropriate packaging aluminised. After the process of irradiation the samples were stored at a temperature of 15 ± 1 deg C and relative humidity of 17 ± 1%. The following analyzes were performed: levels of total sugars, glucose, sucrose, caffeine, humidity, pH, total acidity, electrical conductivity and fibers. Analyses were performed 1, 30, 60 and 90 days after irradiation, and the results were submitted to analysis of variance and means were compared by Tukey test at 5%. It was observed that the analysis results of the samples irradiated with 5 kGy and 10 kGy showed values similar to the control. It was concluded that irradiation did not induce deleterious effects on arabica coffee seeds and conillon irradiated with 5 kGy and 10 kGy to 90 days after irradiation. (author)

  14. Perdas causadas por Coccus viridis (Hemiptera: Coccidae em mudas de Coffea arabica L.

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    Flávio Lemes Fernandes

    2009-08-01

    Full Text Available Coccus viridis (Green danifica plantas jovens e adultas de Coffea arabica Linnaeu. No entanto, nada se sabe sobre a magnitude dos danos causados por esta praga. Assim, este trabalho teve por objetivo estudar as relações entre o ataque de C. viridis e as perdas causadas por este inseto a C. arabica. Este trabalho foi conduzido em casa de vegetação na Universidade Federal de Viçosa. Foram utilizadas sementes da linhagem IAC 15 da variedade “Catuaí vermelho” de café (C. arabica. Para a confecção dos tratamentos esta praga foi criada em casa de vegetação separada do experimento. Os tratamentos foram: plantas infestadas e não infestadas por adultos e ninfas da cochonilha verde. As plantas foram nutridas com solução nutritiva. Durante 110 dias foram avaliados: números de adultos e de ninfas de primeiro, segundo e terceiro ínstares, área foliar, diâmetro do caule, altura das plantas em todas repetições. No final do experimento avaliou-se o peso das raízes, caule, folhas e total. Os pesos das raízes, matéria seca total, área foliar e diâmetro do caule de plantas não atacadas por C. viridis superaram em 1,31; 1,41; 1,50 e 8,93 vezes, respectivamente o peso de plantas atacadas. As variáveis selecionadas foram: diâmetro do caule (cm, área foliar (cm², peso de raízes (g, ninfas, adultos e total das cochonilhas. Concluindo que a planta de C. arabica é afetada de forma diferente entre seus órgãos e que a ninfa de terceiro ínstar e adultos são as fases que mais causam danos a C. arabica.Losses Caused by Coccus viridis (Green (Hemiptera: Coccidae on Seedlings of Coffea arabica L.Abstract. Coccus viridis (Green cause losses on seedling and old plants of Coffea arabica (Green. However, nothing is known about of the damages caused by this pest. Thus, the objective of this work was to evaluate the relations between atack of C. viridis and the losses caused by this insect. on C. arabica. This work was conduced in greenhouse at

  15. Production of herbicide-resistant coffee plants (Coffea canephora P. via Agrobacterium tumefaciens-mediated transformation

    Directory of Open Access Journals (Sweden)

    Alessandra Ferreira Ribas

    2006-01-01

    Full Text Available Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supplemented with 5µM N6 - (2-isopentenyl-adenine (2-iP. Ten µM ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed with up to 1600 mg.L-1 of FinaleTM, a herbicide containing glufosinate as the active ingredient, retained their pigmentation and continued to grow normally during ex vitro acclimation.Plantas transgênicas de Coffea canephora P resistentes ao herbicida glufosinato de amônio foram regeneradas a partir de explantes foliares co-cultivados com Agrobacterium tumefaciens EHA105 contendo o plasmídio pCambia3301 que contém os genes bar e uidA ambos sob controle do promotor 35S. Embriogênese somática direta foi induzida no meio contendo ¼ da concentração de macro, metade da concentração de micronutrientes do meio MS, constituintes orgânicos do meio B5 e 30 g.L-1 de sacarose suplementado com 5µM N6 - (2-isopentenil-adenina (2-iP e 10 µM de glufosinato de amônio para seleção de embriões transgênicos putativos. A presença e a integração do gene bar foram confirmados pelas análises de PCR e Southern blot. As plantas transgênicas selecionadas de café, pulverizadas com 1600 mg.L-1 do herbicida FinaleTM que contém glufosinato como ingrediente ativo, mantiveram a coloração e continuaram crescendo normalmente na aclimatação ex vitro.

  16. Sustained photosynthetic performance of Coffea spp. under long-term enhanced [CO2].

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    José C Ramalho

    Full Text Available Coffee is one of the world's most traded agricultural products. Modeling studies have predicted that climate change will have a strong impact on the suitability of current cultivation areas, but these studies have not anticipated possible mitigating effects of the elevated atmospheric [CO2] because no information exists for the coffee plant. Potted plants from two genotypes of Coffea arabica and one of C. canephora were grown under controlled conditions of irradiance (800 μmol m(-2 s(-1, RH (75% and 380 or 700 μL CO2 L(-1 for 1 year, without water, nutrient or root development restrictions. In all genotypes, the high [CO2] treatment promoted opposite trends for stomatal density and size, which decreased and increased, respectively. Regardless of the genotype or the growth [CO2], the net rate of CO2 assimilation increased (34-49% when measured at 700 than at 380 μL CO2 L(-1. This result, together with the almost unchanged stomatal conductance, led to an instantaneous water use efficiency increase. The results also showed a reinforcement of photosynthetic (and respiratory components, namely thylakoid electron transport and the activities of RuBisCo, ribulose 5-phosphate kinase, malate dehydrogenase and pyruvate kinase, what may have contributed to the enhancements in the maximum rates of electron transport, carboxylation and photosynthetic capacity under elevated [CO2], although these responses were genotype dependent. The photosystem II efficiency, energy driven to photochemical events, non-structural carbohydrates, photosynthetic pigment and membrane permeability did not respond to [CO2] supply. Some alterations in total fatty acid content and the unsaturation level of the chloroplast membranes were noted but, apparently, did not affect photosynthetic functioning. Despite some differences among the genotypes, no clear species-dependent responses to elevated [CO2] were observed. Overall, as no apparent sign of photosynthetic down

  17. Sustained Photosynthetic Performance of Coffea spp. under Long-Term Enhanced [CO2

    Science.gov (United States)

    Ramalho, José C.; Rodrigues, Ana P.; Semedo, José N.; Pais, Isabel P.; Martins, Lima D.; Simões-Costa, Maria C.; Leitão, António E.; Fortunato, Ana S.; Batista-Santos, Paula; Palos, Isabel M.; Tomaz, Marcelo A.; Scotti-Campos, Paula; Lidon, Fernando C.; DaMatta, Fábio M.

    2013-01-01

    Coffee is one of the world’s most traded agricultural products. Modeling studies have predicted that climate change will have a strong impact on the suitability of current cultivation areas, but these studies have not anticipated possible mitigating effects of the elevated atmospheric [CO2] because no information exists for the coffee plant. Potted plants from two genotypes of Coffea arabica and one of C. canephora were grown under controlled conditions of irradiance (800 μmol m-2 s-1), RH (75%) and 380 or 700 μL CO2 L-1 for 1 year, without water, nutrient or root development restrictions. In all genotypes, the high [CO2] treatment promoted opposite trends for stomatal density and size, which decreased and increased, respectively. Regardless of the genotype or the growth [CO2], the net rate of CO2 assimilation increased (34-49%) when measured at 700 than at 380 μL CO2 L-1. This result, together with the almost unchanged stomatal conductance, led to an instantaneous water use efficiency increase. The results also showed a reinforcement of photosynthetic (and respiratory) components, namely thylakoid electron transport and the activities of RuBisCo, ribulose 5-phosphate kinase, malate dehydrogenase and pyruvate kinase, what may have contributed to the enhancements in the maximum rates of electron transport, carboxylation and photosynthetic capacity under elevated [CO2], although these responses were genotype dependent. The photosystem II efficiency, energy driven to photochemical events, non-structural carbohydrates, photosynthetic pigment and membrane permeability did not respond to [CO2] supply. Some alterations in total fatty acid content and the unsaturation level of the chloroplast membranes were noted but, apparently, did not affect photosynthetic functioning. Despite some differences among the genotypes, no clear species-dependent responses to elevated [CO2] were observed. Overall, as no apparent sign of photosynthetic down-regulation was found, our data

  18. Anatomia e desenvolvimento ontogenético de Coffea arabica L. var. typica Cramer Anatomy and ontogenetical development of Coffea arabica L. var. typica Cramer

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    D. M. Dedecca

    1957-01-01

    Full Text Available O presente estudo anatômico de Coffea arabica L. var. typica Cramer tem por finalidade fornecer informações básicas necessárias ao estudo da anatomia comparada das principais espécies e variedades de cafeeiros, cultivadas no Estado de São Paulo. Nesta primeira contribuição o autor realiza o estudo anatômico detalhado dos órgãos vegetativos e reprodutivos da variedade typica, não se limitando apenas à anatomia descritiva dos diversos órgãos, mas também, sempre que possível, discutindo o desenvolvimento ontogenético das diversas partes do cafeeiro. No estudo da raíz e do caule procurou-se estabelecer a duração do desenvolvimento primário, assinalando o local de aparecimento, primeiramente do câmbio vascular e, posteriormente, do felógeno ou câmbio suberoso. Na discussão da anatonia das folhas mereceu especial atenção o estudo das domácias, sua morfologia e possível função. As flores são estudadas detalhadamente nos seus diversos elementos. Nos capítulos referentes à anatomia do fruto e da semente, além do estudo puramente descritivo das suas estruturas são ainda discutidas as diversas modificações verificadas durante o desenvolvimento do ovário e dos óvulos, respectivamente em fruto e sementes.The knowledge of the anatomy of Coffea arabica L. var. typica Cramer should be considered as a basic contribution to the comparative study of the anatomy of coffee species and varieties cultivated in the State of São Paulo, Brazil. The detailed investigations carried out on the vegetative and reproductive organs of the coffee plant revealed the following. The roots at the end of the primary growth present a protostelic, poliarch (6, 7, 8, 9, 11 primary xylem bundles, and exarch structure, the following tissues or zone of tissues being visible: root cap, epidermis, exodermis, cortex, endodermis, pericycle, primary phloem and primary xylem. This primary growth has a very short duration and is very soon followed by the

  19. Resistência de plantas de coffea arabica, coffea canephora e híbridos interespecíficos à cercosporiose

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    Flávia Rodrigues Alves Patricio

    2010-12-01

    Full Text Available O presente estudo objetivou avaliar a resistência de plantas de Coffea arabica, C. canephora e de híbridos interespecíficos, à cercosporiose (Cercospora coffeicola, importante doença da cultura do cafeeiro. Foram realizados dois experimentos em 2003, em Campinas (SP, com mudas das cultivares de C. arabica, Mundo Novo IAC 388-17-1, Mundo Novo IAC 376-4, Bourbon Amarelo, Bourbon Vermelho, Obatã IAC 1669-20, Catuaí Vermelho IAC 144, Catuaí Amarelo IAC 62, Icatu Precoce IAC 3282, Icatu Vermelho IAC 4045, Ouro Verde IAC H5010-5 e Tupi IAC 1669-33; de C. canephora, Robusta IAC 1653-7 e Apoatã IAC 2258, além de dois híbridos interespecíficos Piatã IAC 387 e Híbrido de Timor IAC 1559-13. As mudas foram submetidas à inoculação com uma suspensão de conídios preparada com cinco isolados coletados em diferentes regiões do Estado de São Paulo. Os materiais mais suscetíveis foram Robusta IAC 1653-7, Apoatã IAC 2258, Híbrido de Timor IAC 1559-13, Bourbon Vermelho e Bourbon Amarelo com incidência entre 63,1% e 81,6% no primeiro experimento e entre 70% e 93,3% no segundo e severidade (notas 1-6 entre 2,92 e 3,42 no primeiro experimento e entre 3,64 e 2,79 no segundo. Os materiais mais resistentes foram Piatã IAC 387, Ouro Verde IAC H5010-5 e Tupi IAC 1669-33, com incidência entre 28,4% e 35,5% no primeiro experimento e 21,1% e 55,6% no segundo, e severidade entre 1,44 e 1,73 e entre 1,28 e 1,88, respectivamente, no primeiro e segundo experimentos.

  20. Influence of the fungi population on the physicochemical and chemical composition of coffee (Coffea arabica L.

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    Liliana Auxiliadora Avelar Pereira Pasin

    2011-09-01

    Full Text Available The influence of fungi associated with coffee fruits was verified regarding the chemical and physicochemical composition of Coffea arabica L. raw grains. The fruits were harvested at EPAMIG Experimental farm in Lavras, State of Minas Gerais - making up the different samples here analyzed. After processing and drying, the grains were incubated in wet chamber for fungal exteriorization through the blotter test method and submitted to the following analyses: polyphenoloxidase, total reducing and non-reducing sugars, clorogenic acid, titrable acidity, potassium leaching, electric conductivity and caffeine. The occurrence of the P. variable, P. rugulosum, P. funiculosum, F. equiseti, F. semitectum, A.alutaceus, A. niger and C. cladosporioides fungi in the different samples was detected. From the analysis of the results obtained, it was observed that the presence of the Aspergillus alutaceus fungus reduces the activity of the enzyme polyphenoloxidase and increases the values of potassium leaching, electric conductivity and chlorogenic acid. The incidence of the Cladosporium cladosporioides fungus influenced the average values of potassium leaching and electric conductivity.

  1. Vegetation classification of Coffea on Hawaii Island using WorldView-2 satellite imagery

    Science.gov (United States)

    Gaertner, Julie; Genovese, Vanessa Brooks; Potter, Christopher; Sewake, Kelvin; Manoukis, Nicholas C.

    2017-10-01

    Coffee is an important crop in tropical regions of the world; about 125 million people depend on coffee agriculture for their livelihoods. Understanding the spatial extent of coffee fields is useful for management and control of coffee pests such as Hypothenemus hampei and other pests that use coffee fruit as a host for immature stages such as the Mediterranean fruit fly, for economic planning, and for following changes in coffee agroecosystems over time. We present two methods for detecting Coffea arabica fields using remote sensing and geospatial technologies on WorldView-2 high-resolution spectral data of the Kona region of Hawaii Island. The first method, a pixel-based method using a maximum likelihood algorithm, attained 72% producer accuracy and 69% user accuracy (68% overall accuracy) based on analysis of 104 ground truth testing polygons. The second method, an object-based image analysis (OBIA) method, considered both spectral and textural information and improved accuracy, resulting in 76% producer accuracy and 94% user accuracy (81% overall accuracy) for the same testing areas. We conclude that the OBIA method is useful for detecting coffee fields grown in the open and use it to estimate the distribution of about 1050 hectares under coffee agriculture in the Kona region in 2012.

  2. Relationship between aluminum stress and caffeine biosynthesis in suspension cells of Coffea arabica L.

    Science.gov (United States)

    Pech-Kú, Roberto; Muñoz-Sánchez, J Armando; Monforte-González, Miriam; Vázquez-Flota, Felipe; Rodas-Junco, Beatriz A; González-Mendoza, Víctor M; Hernández-Sotomayor, S M Teresa

    2018-04-01

    Toxicity by aluminum is a growth-limiting factor in plants cultivated in acidic soils. This metal also promotes signal transduction pathways leading to the biosynthesis of defense compounds, including secondary metabolites. In this study, we observed that Coffea arabica L. cells that were kept in the dark did not produce detectable levels of caffeine. However, irradiation with light and supplementation of the culture medium with theobromine were the best conditions for cell maintenance to investigate the role of aluminum in caffeine biosynthesis. The addition of theobromine to the cells did not cause any changes to cell growth and was useful for the bioconversion of theobromine to caffeine. During a short-term AlCl 3 -treatment (500μM) of C. arabica cells kept under light irradiation, increases in the caffeine levels in samples that were recovered from both the cells and culture media were evident. This augmentation coincided with increases in the enzyme activity of caffeine synthase (CS) and the transcript level of the gene encoding this enzyme (CS). Together, these results suggest that actions by Al and theobromine on the same pathway lead to the induction of caffeine biosynthesis. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. Genetic diversity among 16 genotypes of Coffea arabica in the Brazilian cerrado.

    Science.gov (United States)

    Machado, C M S; Pimentel, N S; Golynsk, A; Ferreira, A; Vieira, H D; Partelli, F L

    2017-09-21

    For the selection of coffee plants that have favorable characteristics, it is necessary to evaluate variables related to production. Knowledge of the genetic divergence of arabica coffee is of extreme importance, as this knowledge can be associated with plant breeding programs in order to combine genetic divergence with good productive performance. The objective of this study was to evaluate the genetic divergence among 16 genotypes of Coffea arabica with the purpose of identifying the most dissimilar genotypes for the establishment of breeding programs and adaptation to the Brazilian cerrado. The genetic divergence was evaluated using multivariate procedures, the analysis of the average grouping unweighted pair group method with arithmetic mean (UPGMA) and main components in 2013 and 2014. Eight characters were evaluated in an experiment conducted in Morrinhos, Goiás. The presence of genetic divergence among the 16 C. arabica genotypes under cerrado conditions was recorded. The formation of UPGMA groups for the evaluated characteristics was pertinent due to the number of genotypes. The first three major components accounted for 81.77% of the total variance. The genotype H-419-3-4-4-13(C-241) of low size was the most divergent, followed by Catucaí 2 SL and Catiguá MG2, according to the main components.

  4. Morphological Characterization and Identification of Coffea liberica Callus of Somatic Embryogenesis Propagation.

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    Fitria Ardiyani

    2015-08-01

    Full Text Available Compared with other types of coffee, Liberica coffee is more difficult to be propagates using clonal methods. Meanwhile, demand for planting materials and consumption of this type of coffee is increasing lately. The objective of this paper is to present results of the work on morpological characterization of Liberica coffee (Coffea liberica callus produced by somatic embryogenesis propagation. This research used C. liberica Arruminensis clone. This clone was one of Liberica coffee clones which had superior taste. Main activitis carried out in this experiment were explant sterilization, explant induction and histological analysis on the callus produced. The result of this research showed that non embryogenic callus was higher (72% than embryogenic callus (28%. The callus description can be used to identify type and characteristic of the callus. Therefore, it can be a parameter to choose type of callus for propagation material. This is important because choosing the right callus is determine of the succesfully process of Liberica somatic embryogenesis. Keywords: somatic embryogenesis, Liberica, embryogenic, non-embryogenic

  5. Galactinol synthase transcriptional profile in two genotypes of Coffea canephora with contrasting tolerance to drought

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    Tiago Benedito Dos Santos

    2015-06-01

    Full Text Available Increased synthesis of galactinol and raffinose family oligosaccharides (RFOs has been reported in vegetative tissues in response to a range of abiotic stresses. In this work, we evaluated the transcriptional profile of a Coffea canephora galactinol synthase gene (CcGolS1 in two clones that differed in tolerance to water deficit in order to assess the contribution of this gene to drought tolerance. The expression of CcGolS1 in leaves was differentially regulated by water deficit, depending on the intensity of stress and the genotype. In clone 109A (drought-susceptible, the abundance of CcGolS1 transcripts decreased upon exposure to drought, reaching minimum values during recovery from severe water deficit and stress. In contrast, CcGolS1 gene expression in clone 14 (drought-tolerant was stimulated by water deficit. Changes in galactinol and RFO content did not correlate with variation in the steady-state transcript level. However, the magnitude of increase in RFO accumulation was higher in the tolerant cultivar, mainly under severe water deficit. The finding that the drought-tolerant coffee clone showed enhanced accumulation of CcGolS1 transcripts and RFOs under water deficit suggests the possibility of using this gene to improve drought tolerance in this important crop.

  6. Variation of Potential Yield of Hybrid Population of Robusta coffee (Coffea canepor

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    Novie Pranata Erdiansyah

    2014-08-01

    Full Text Available The low yield of Robusta coffee in Indonesia may be due to the use of planting materials derived from seeds. The research objective was to determine the variation of Robusta coffee yield wich local propagated by using seeds. The study was conducted in Kaliwining experimental Station of ICCRI (Indonesian Coffee andCocoa Research Institute. There were two populations observed. Number of progeny used in this study were 186 genotypes consisting of two groups from crossesBP 409 x Q 121 with 89 progenies and BP 961 x BP 409 with 81 progenies. The results showed that planting materials from seeds exhibit properties mixed results.Progeny that have the best results (yield more than 2 ton/ha not more than 5% of the total population. In both populations there is a big difference between the progenythat has high and low yield. Highest yield B population could reach 2,500 kg/ha and the C population reached 2,200 kg/ha. The lowest yield can only produce coffee270 kg/ha in populations B and 120 kg/ha in population C.Key words: Coffea canephora, hybrid, variation, yield

  7. Comportamiento productivo de clones de café robusta (Coffea Canephora p en Manglaralto, Ecuador.

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    Mercedes Arzube Mayorga

    2017-05-01

    Abstract The research was carried out in the experimental farm Manglaralto, owned by the Peninsula State University of Santa Elena, located at the coordinates UTM 528944m E and 9796468m S zone 17M datum WGS-84 at 12 msnm, with topography of less than 1%, research (Coffea canephora P., high productivity in the agroecological conditions of Manglaralto Ecuador. For the establishment of the trial, 23 clones of robust coffee, selected by COFENAC in the Amazon region of northern Ecuador, were used. The clones were arranged randomly, each clone is an experimental unit represented by 20 plants, planted at a distance of 3 x 3 meters. Preliminary results were submitted to the descriptive statistics analysis, determining measures of central tendency and mean arithmetic dispersion, standard deviation, coefficient of variation, between clones and within the clones. However, in the fourth year, clones 1, 4, 5, 6, 14, 15, 16 and 18 stand out as promising in production. The productive behavior is very encouraging considering that clone 1 obtained production of 61 quintals and the clone 15 reached 39.3 quintals of gold coffee per hectare, the other clones enunciated obtain average production of 42 quintals.

  8. Estimation of leaf area in coffee leaves (Coffea arabica L. of the Castillo® variety

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    Carlos Andrés Unigarro-Muñoz

    2015-01-01

    Full Text Available Allometric models based on measurements of single leaf dimensions or a combination there are useful tools for determining individual leaf area (LA because they are non-destructive, precise, simple and economical methods. The present study was carried out at the Central Station Naranjal of Cenicafé, located in the Department of Caldas (Colombia, four models were defined using the variables length (L and/or width (W to estimate LA in coffee leaves of the Castillo® variety (Coffea arabica L.. Estimation of regression coefficients was performed using information recorded from 6,441 leaves (group 1, and their validation was performed using records from another 992 leaves (group 2. Leaves were collected from all strata of the canopy and ranged from 0.76 to 140 cm2 in LA. In addition to exhibiting coefficients of variation differing from zero based on t-tests at 1%, the evaluated models possess coefficients of determination between 0.93 and 0.99. Four expressions have developed and adjusted to estimate leaf area in individual leaves, based on the measurement of simple variables and non-destructive.

  9. Ovarian egg morphology in chalcidoid wasps (Hymenoptera: Chalcidoidea parasitizing gall wasps (Hymenoptera: Cynipidae

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    Vårdal, H.

    2016-06-01

    Full Text Available We provide morphological egg data of 26 species of 5 chalcidoid families associated with cynipid galls (Hymenoptera: Cynipidae from western Palaearctic, including the first egg data for the family Ormyridae. Adult chalcidoid species were reared from galls, and eggs obtained from dissected female ovaries were examined using scanning electron microscopy (SEM. The shape of the eggs varies from oval to elongate and tapered at both ends. Eggs of Eurytomidae as well as some Eulophidae, Eupelmidae and Pteromalidae are equipped with a peduncle at the anterior end. We found a positive correlation between long eggs and long ovipositors and confirmed the expectation that eggs of endoparasitoids are generally shorter and narrower than eggs of ectoparasitoids. We were able to locate the sperm entrance or micropyle at the anterior pole of eggs of several species. It is situated at the anterior end of the egg and at the end of the peduncle when present. In addition, the eggshells of the endoparasitoid Sycophila biguttata (Swederus, 1795 (Hymenoptera: Eurytomidae and the ectoparasitoid Cecidostiba fungosa (Geoffroy, 1785 (Hymenoptera: Pteromalidae, are for the first time described.En el presente trabajo se aportan datos morfol.gicos del huevo de 26 especies del Paleártico occidental pertenecientes a 5 familias de Chalcidoidea asociadas con agallas de cinípidos (Hymenoptera: Cynipidae, incluyendo los primeros datos del huevo de especies de Ormyridae. Los ejemplares adultos de las especies estudiadas fueron obtenidos por emergencia de agallas en laboratorio, los ovarios de las hembras diseccionados para obtener los huevos, que fueron finalmente estudiados utilizando técnicas de microscopía electronica de barrido. La forma de los huevos estudiados varía de ovalada a alargada y ahusada en ambos extremos. Los huevos de Eurytomidae, así como algunos de Eulophidae, Eupelmidae y Pteromalidae están provistos de un pedúnculo en el extremo anterior. Se encontr

  10. Biochemical and molecular characterization of the antioxidative system of Coffea sp. under cold conditions in genotypes with contrasting tolerance.

    Science.gov (United States)

    Fortunato, Ana S; Lidon, Fernando C; Batista-Santos, Paula; Leitão, António Eduardo; Pais, Isabel P; Ribeiro, Ana I; Ramalho, José Cochicho

    2010-03-15

    Low positive temperature (chilling) is frequently linked to the promotion of oxidative stress conditions, and is of particular importance in the coffee plant due to its severe impact on growth, development, photosynthesis and production. Nevertheless, some acclimation ability has been reported within the Coffea genus, and is possibly related to oxidative stress control. Using an integrated biochemical and molecular approach, the characterization of the antioxidative system of genotypes with different cold acclimation abilities was performed. Experiments were carried out using 1.5-year-old coffee seedlings of Coffea canephora cv. Apoatã, C. arabica cv. Catuaí, C. dewevrei and 2 hybrids, Icatu (C. arabicaxC. canephora) and Piatã (C. dewevreixC. arabica) subjected to a gradual cold treatment and a recovery period. Icatu showed the greatest ability to control oxidative stress, as reflected by the enhancement of several antioxidative components (Cu,Zn-SOD and APX activities; ascorbate, alpha-tocopherol and chlorogenic acids (CGAs) contents) and lower reactive oxygen species contents (H(2)O(2) and OH). Gene expression studies show that GRed, DHAR and class III and IV chitinases might also be involved in the cold acclimation ability of Icatu. Catuaí showed intermediate acclimation ability through the reinforcement of some antioxidative molecules, usually to a lesser extent than that observed in Icatu. On the other hand, C. dewevrei showed the poorest response in terms of antioxidant accumulation, and also showed the greatest increase in OH values. The difference in the triggering of antioxidative traits supports the hypothesis of its importance to cold (and photoinhibition) tolerance in Coffea sp. and could provide a useful probe to identify tolerant genotypes. Copyright 2009 Elsevier GmbH. All rights reserved.

  11. The Greater Phenotypic Homeostasis of the Allopolyploid Coffea arabica Improved the Transcriptional Homeostasis Over that of Both Diploid Parents.

    Science.gov (United States)

    Bertrand, Benoît; Bardil, Amélie; Baraille, Hélène; Dussert, Stéphane; Doulbeau, Sylvie; Dubois, Emeric; Severac, Dany; Dereeper, Alexis; Etienne, Hervé

    2015-10-01

    Polyploidy impacts the diversity of plant species, giving rise to novel phenotypes and leading to ecological diversification. In order to observe adaptive and evolutionary capacities of polyploids, we compared the growth, primary metabolism and transcriptomic expression level in the leaves of the newly formed allotetraploid Coffea arabica species compared with its two diploid parental species (Coffea eugenioides and Coffea canephora), exposed to four thermal regimes (TRs; 18-14, 23-19, 28-24 and 33-29°C). The growth rate of the allopolyploid C. arabica was similar to that of C. canephora under the hottest TR and that of C. eugenioides under the coldest TR. For metabolite contents measured at the hottest TR, the allopolyploid showed similar behavior to C. canephora, the parent which tolerates higher growth temperatures in the natural environment. However, at the coldest TR, the allopolyploid displayed higher sucrose, raffinose and ABA contents than those of its two parents and similar linolenic acid leaf composition and Chl content to those of C. eugenioides. At the gene expression level, few differences between the allopolyploid and its parents were observed for studied genes linked to photosynthesis, respiration and the circadian clock, whereas genes linked to redox activity showed a greater capacity of the allopolyploid for homeostasis. Finally, we found that the overall transcriptional response to TRs of the allopolyploid was more homeostatic compared with its parents. This better transcriptional homeostasis of the allopolyploid C. arabica afforded a greater phenotypic homeostasis when faced with environments that are unsuited to the diploid parental species. © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  12. Head capsule characters in the Hymenoptera and their phylogenetic implications

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    Lars Vilhelmsen

    2011-09-01

    Full Text Available The head capsule of a taxon sample of three outgroup and 86 ingroup taxa is examined for characters of possible phylogenetic significance within Hymenoptera. 21 morphological characters are illustrated and scored, and their character evolution explored by mapping them onto a phylogeny recently produced from a large morphological data set. Many of the characters are informative and display unambiguous changes. Most of the character support demonstrated is supportive at the superfamily or family level. In contrast, only few characters corroborate deeper nodes in the phylogeny of Hymenoptera.

  13. Consequences of Stoichiometric Error on Nuclear DNA Content Evaluation in Coffea liberica var. dewevrei using DAPI and Propidium Iodide

    OpenAIRE

    NOIROT, MICHEL; BARRE, PHILIPPE; LOUARN, JACQUES; DUPERRAY, CHRISTOPHE; HAMON, SERGE

    2002-01-01

    The genome size of coffee trees (Coffea sp.) was assessed using flow cytometry. Nuclear DNA was stained with two dyes [4′,6‐diamino‐2‐phenylindole dihydrochloride hydrate (DAPI) and propidium iodide (PI)]. Fluorescence in coffee tree nuclei (C‐PI or C‐DAPI) was compared with that of the standard, petunia (P‐PI or P‐DAPI). If there is no stoichiometric error, then the ratio between fluorescence of the target nuclei and that of the standard nuclei (R‐PI or R‐DAPI) is expected to be proportional...

  14. Primera cita de la Argentina de Ophelimus maskelli (Ashmead (Hymenoptera: Eulophidae y su parasitoide, Closterocerus chamaeleon (Girault (Hymenoptera: Eulophidae

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    Daniel A AQUINO

    2014-01-01

    Full Text Available Se cita por primera vez de la Argentina la presencia de la avispa galí - cola exótica Ophelimus maskelli (Ashmead (Hymenoptera: Eulophidae y su para - sitoide natural, Closterocerus chamaeleon (Girault (Hymenoptera: Eulophidae en Eucalyptus camaldulensis de la provincia de Buenos Aires. Ophelimus maskelli es una especie invasora de origen Australiano que ha sido reportada como plaga de los eucaliptos en numerosos países de Asia, Europa y América. Closterocerus chamaleon es un parasitoide exótico cuyo potencial como biocontrolador de O. maskelli está siendo evaluado en algunos países.

  15. Natural history of interaction between Meteorus sp. Haliday, 1835 (Hymenoptera: Braconidae and its hyperparasitoid Toxeumella albipes Girault, 1913 (Hymenoptera: Pteromalidae

    Directory of Open Access Journals (Sweden)

    JF Sobczak

    Full Text Available Some parasitoids build a cocoon mass that hangs in the host body until the adults emergence, which is an advantage against attack by predators who troll the vegetation in search of prey. However, such behaviour is not effective against the hyperparasitoid attacks. This study reports the interaction between the caterpillar Manduca sexta Linnaeus, 1763 (Lepidoptera, Sphingidae parasitised by Meteorus sp. (Hymenoptera, Braconidae larvae and its hyperparasitoid Toxeumella albipes (Hymenoptera, Pteromalidae. This is the first description of the attack and oviposition of T. albipes.

  16. Hymenoptera stings in Brazil: a neglected health threat in Amazonas State

    Directory of Open Access Journals (Sweden)

    Allyson Guimarães Costa

    Full Text Available Abstract INTRODUCTION: Hymenoptera injuries are commonly caused by stinging insects. In Amazonas state, Brazil, there is no information regarding distribution, profile, and systemic manifestations associated with Hymenoptera injuries. METHODS: This study aimed to identify risk factors for systemic manifestation using the Brazilian Notifiable Diseases Surveillance System (2007 to 2015. RESULTS: Half of Hymenoptera injuries were caused by bee stings. Hymenoptera injuries were concentrated in Manaus, and 13.36% of cases displayed systemic signs. Delayed medical assistance (4 to 12 hours presented four times more risk for systemic manifestations. CONCLUSIONS: Simple clinical observations and history of injury are critical information for prognostic improvement.

  17. Use of coffee (Coffea arabica pulp for the production of briquettes and pellets for heat generation

    Directory of Open Access Journals (Sweden)

    Robert Cubero-Abarca

    2014-10-01

    Full Text Available Coffee bean (Coffea arabica processing generates high amount of residues that are sources of environmental pollution. Therefore, an appropriate solution is needed. The objective of this study was to determine the potential of coffee pulp to produce briquettes and pellets. The study included pulp drying (using air, solar and hot air methods; the production of briquettes and pellets; the evaluation of their energy, physical and mechanical properties; and the evaluation of pellet quality using X-ray densitometry. The results showed that the pulp presented an initial moisture content of 90%, resulting in drying times of 699, 308 and 55 hours for air, solar and hot air drying, respectively, and the calorific values of the pellets and briquettes were 12,501 kJ kg-1 and 11,591 kJ kg-1, respectively. The ash content was 8.68% for the briquettes and 6.74% for the pellets. The density of the briquettes was 1,110 kg m-3, compared with 1,300 kg m-3 for the pellets. The apparent densities were 1,000 kg m-3 and 600 kg m-3 for the briquettes and pellets, respectively, and the water absorptions by the briquettes were 7.90% and 8.10% by the pellets. The maximum horizontal compression effort was 26.86 kg cm-2, measured in the pellets, compared with 4.52 kg cm-2 in the briquettes. The maximum horizontal load was 93.24 kg, measured in the briquettes, compared with 33.50 kg in the pellets. The value of the pellet durability test was 75.54%. X-ray densitometry showed that the pellet was uniform and a few cracks were observed on the pellet surface.

  18. Biomass production by Coffea canephora Pierre ex Froehner in two productives cycles

    International Nuclear Information System (INIS)

    Bustamante González, Carlos; Rodríguez, Maritza I.; Pérez Díaz, Alberto; Viñals, Rolando; Martín Alonso, Gloria M.; Rivera, Ramón

    2015-01-01

    In areas of the Estación Central de Investigaciones de Café y Cacao located in La Mandarina, Tercer Frente municipality, Santiago de Cuba province, and La Alcarraza, municipality Sagua de Tánamo, Holguín province, the biomass production of Coffea canephora Pierre ex Froehner var. Robusta was assessed from planting until the fourth year in both locations and after pruning until the fourth year in Alcarraza. The coffee trees were planted at 3 x 1,5 m in Cambisol under Samanea saman Jerr shade in the first town and Leucaena leucocephala Lam de Wit in the second. The biomass was separated into: leaves, branches, stems, fruits and roots. From 24 months and one year after pruning, leaflitter was collected monthly. For the study of the root system soil blocks of 25 x 25 x 25 cm were extracted, in an area formed by 1,5 m (distance to the street) and 0,75 m (between plants), centered relative to the coffee plant and up to a meter deep. The extracted soil represented ¼ of the volume occupied by the plant. The dry mass of each organ was determined. Dry matter production reached values of 25 t dry mass ha-1 regardless of the stage of the plantation. Until the fourth year the root system dominated the biomass, followed by the leaves and then the stems. The participation of the fruits in the biomass increased in the crop stage and when concluding the experiment the coffees had dedicated for its formation among the 16-20 % of the total dry mass, independently of the development cycle. (author)

  19. Use of a draft genome of coffee (Coffea arabica) to identify SNPs associated with caffeine content.

    Science.gov (United States)

    Tran, Hue T M; Ramaraj, Thiruvarangan; Furtado, Agnelo; Lee, Leonard Slade; Henry, Robert J

    2018-03-07

    Arabica coffee (Coffea arabica) has a small gene pool limiting genetic improvement. Selection for caffeine content within this gene pool would be assisted by identification of the genes controlling this important trait. Sequencing of DNA bulks from 18 genotypes with extreme high- or low-caffeine content from a population of 232 genotypes was used to identify linked polymorphisms. To obtain a reference genome, a whole genome assembly of arabica coffee (variety K7) was achieved by sequencing using short read (Illumina) and long-read (PacBio) technology. Assembly was performed using a range of assembly tools resulting in 76 409 scaffolds with a scaffold N50 of 54 544 bp and a total scaffold length of 1448 Mb. Validation of the genome assembly using different tools showed high completeness of the genome. More than 99% of transcriptome sequences mapped to the C. arabica draft genome, and 89% of BUSCOs were present. The assembled genome annotated using AUGUSTUS yielded 99 829 gene models. Using the draft arabica genome as reference in mapping and variant calling allowed the detection of 1444 nonsynonymous single nucleotide polymorphisms (SNPs) associated with caffeine content. Based on Kyoto Encyclopaedia of Genes and Genomes pathway-based analysis, 65 caffeine-associated SNPs were discovered, among which 11 SNPs were associated with genes encoding enzymes involved in the conversion of substrates, which participate in the caffeine biosynthesis pathways. This analysis demonstrated the complex genetic control of this key trait in coffee. © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  20. Application of thermography for monitoring stomatal conductance of Coffea arabica under different shading systems.

    Science.gov (United States)

    Craparo, A C W; Steppe, K; Van Asten, P J A; Läderach, P; Jassogne, L T P; Grab, S W

    2017-12-31

    Stomatal regulation is a key process in the physiology of Coffea arabica (C. arabica). Intrinsically linked to photosynthesis and water relations, it provides insights into the plant's adaptive capacity, survival and growth. The ability to rapidly quantify this parameter for C. arabica under different agroecological systems would be an indispensable tool. Using a Flir E6 MIR Camera, an index that is equivalent to stomatal conductance (I g ) was compared with stomatal conductance measurements (g s ) in a mature coffee plantation. In order to account for varying meteorological conditions between days, the methods were also compared under stable meteorological conditions in a laboratory and I g was also converted to absolute stomatal conductance values (g 1 ). In contrast to typical plant-thermography methods which measure indices once per day over an extended time period, we used high resolution hourly measurements over daily time series with 9 sun and 9 shade replicates. Eight daily time series showed a strong correlation between methods, while the remaining 10 were not significant. Including several other meteorological parameters in the calculation of g 1 did not contribute to any stronger correlation between methods. Total pooled data (combined daily series) resulted in a correlation of ρ=0.66 (P≤2.2e-16), indicating that our approach is particularly useful for situations where absolute values of stomatal conductance are not required, such as for comparative purposes, screening or trend analysis. We use the findings to advance the protocol for a more accurate methodology which may assist in quantifying advantageous microenvironment designs for coffee, considering the current and future climates of coffee growing regions. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  1. Genetic molecular analysis of Coffea arabica (Rubiaceae hybrids using SRAP markers

    Directory of Open Access Journals (Sweden)

    Manoj Kumar Mishra

    2011-06-01

    Full Text Available In Coffea arabica (arabica coffee, the phenotypic as well as genetic variability has been found low because of the narrow genetic basis and self fertile nature of the species. Because of high similarity in phenotypic appearance among the majority of arabica collections, selection of parental lines for inter-varietals hybridization and identification of resultant hybrids at an early stage of plant growth is difficult. DNA markers are known to be reliable in identifying closely related cultivars and hybrids. Sequence Related Amplified Polymorphism (SRAP is a new molecular marker technology developed based on PCR. In this paper, sixty arabica-hybrid progenies belonging to six crosses were analyzed using 31 highly polymorphic SRAP markers. The analysis revealed seven types of SRAP marker profiles which are useful in discriminating the parents and hybrids. The number of bands amplified per primer pair ranges from 6.13 to 8.58 with average number of seven bands. Among six hybrid combinations, percentage of bands shared between hybrids and their parents ranged from 66.29% to 85.71% with polymorphic bands varied from 27.64% to 60.0%. Percentage of hybrid specific fragments obtained in various hybrid combinations ranged from 0.71% to 10.86% and ascribed to the consequence of meiotic recombination. Based on the similarity index calculation, it was observed that F1 hybrids share maximum number of bands with the female parent compared to male parent. The results obtained in the present study revealed the effectiveness of SRAP technique in cultivar identification and hybrid analysis in this coffee species. Rev. Biol. Trop. 59 (2: 607-617. Epub 2011 June 01.

  2. A preliminary checklist of the ants (Hymenoptera: Formicidae) of ...

    African Journals Online (AJOL)

    A preliminary species checklist of the ants (Hymenoptera: Formicidae) of. Kakamega Forest, Western Kenya, is presented. The species list is based on specimens sampled from 1999 until 2009, which are deposited in the ant collection of the Zoological Research Museum Koenig, Bonn, Germany, and the Natural History ...

  3. Six new genera of Braconidae (Hymenoptera) from China

    NARCIS (Netherlands)

    Achterberg, van C.; Chen, X.

    2004-01-01

    Six new genera of the family Braconidae (Hymenoptera) from China are described and illustrated: two genera of the subfamily Agathidinae: Facilagathis gen. nov. (type species: F. spinulata spec. nov.) and Cremnoptoides gen. nov. (type species: Cremnops pappi Sharkey, 1994); one genus of the subfamily

  4. On the Hymenoptera (exclusive of the Anthophila and Formicidae)

    NARCIS (Netherlands)

    Cameron, P.

    1913-01-01

    Our knowledge of the Hymenoptera of the Island of Waigeu hitherto has been based on the material collected by Dr. A. R. Wallace and described by Mr. Frederick Smith in the Journal of the Linnean Society of London, 1863, pp. 6—48. Omitting the bees (5 species) and the ants (27 species) Smith

  5. An anocellar polistine wasp (Hymenoptera, Vespidae, Polistinae from Texas

    Directory of Open Access Journals (Sweden)

    Volker Lohrmann

    2016-12-01

    Full Text Available A remarkable teratological female of Polistes (Fuscopolistes dorsalis neotropicus Bequaert, 1940 (Vespidae: Polistinae is described and illustrated. The specimen lacks all three external dorsal ocelli but is normally developed in almost every other aspect. Additionally, similar findings in other Hymenoptera are briefly discussed, as are the consequences and the reasons that might cause the random loss of ocelli.

  6. Provisional host catalogue of Fig wasps (Hymenoptera, Chalcidoidea)

    NARCIS (Netherlands)

    Wiebes, J.T.

    1966-01-01

    INTRODUCTION In this catalogue — entitled "provisional" because our knowledge of the subject is still so evidently incomplete — all species of Ficus mentioned as hosts of fig wasps, are listed with the Hymenoptera Chalcidoidea reared from their receptacles. The names used for the Agaonidae are in

  7. De urntjeswesp Eumenes coronatus zoekt het hogerop (Hymenoptera: Vespidae)

    NARCIS (Netherlands)

    Smit, J.

    2005-01-01

    The potter wasp Eumenes coronatus on the move (Hymenoptera: Vespidae) The first record of Eumenus coronatus in the Netherlands dates back from 1954. After that the species was not recorded until 1988. Since then many specimens were collected in the southern part of the country. In 2004 a female was

  8. Effects of Apis mellifera adansonii, L. 1758 (Apidae: Hymenoptera ...

    African Journals Online (AJOL)

    Effects of Apis mellifera adansonii, L. 1758 (Apidae: Hymenoptera) pollination on yields of Cucumeropsis mannii (Naudin) in Kisangani, Democratic Republic of Congo. Boniface Posho Ndola, Yves Brostaux, Guillaume Le Goff, Marie-Lucie Susini, Eric Haubruge, Frederic Francis, Bach Kim Nguyen ...

  9. Revision of the world species of Xeris Costa (Hymenoptera: Siricidae)

    Science.gov (United States)

    Henri Goulet; Caroline Boudreault; Nathan M. Schiff

    2015-01-01

    Xeris is one of ten extant genera of Siricidae known as as woodwasps or horntails. They are important wood-boring Hymenoptera from the Northern Hemisphere. Adults and larvae of Xeris are often intercepted at ports and are consequently of concern as potential alien invasive species. The genus consists of 16 species with eight in...

  10. In-vitro diagnostics of Hymenoptera venom allergy

    NARCIS (Netherlands)

    Rueff, F.; Vos, B.; Przybilla, B.

    In-vitro diagnostics of Hymenoptera venom allergy Patients with a history of anaphylactic sting reactions require an allergological work-up (history, in-vitro tests, and skin tests) to clarify indications on venom immunotherapy and on the type of venom to be used. To demonstrate a venom

  11. Genetic Diversity of Arabica Coffee (Coffea arabica L. in Nicaragua as Estimated by Simple Sequence Repeat Markers

    Directory of Open Access Journals (Sweden)

    Mulatu Geleta

    2012-01-01

    Full Text Available Coffea arabica L. (arabica coffee, the only tetraploid species in the genus Coffea, represents the majority of the world’s coffee production and has a significant contribution to Nicaragua’s economy. The present paper was conducted to determine the genetic diversity of arabica coffee in Nicaragua for its conservation and breeding values. Twenty-six populations that represent eight varieties in Nicaragua were investigated using simple sequence repeat (SSR markers. A total of 24 alleles were obtained from the 12 loci investigated across 260 individual plants. The total Nei’s gene diversity (HT and the within-population gene diversity (HS were 0.35 and 0.29, respectively, which is comparable with that previously reported from other countries and regions. Among the varieties, the highest diversity was recorded in the variety Catimor. Analysis of variance (AMOVA revealed that about 87% of the total genetic variation was found within populations and the remaining 13% differentiate the populations (FST=0.13; P<0.001. The variation among the varieties was also significant. The genetic variation in Nicaraguan coffee is significant enough to be used in the breeding programs, and most of this variation can be conserved through ex situ conservation of a low number of populations from each variety.

  12. Brachymeria pandora (Crawford (Hymenoptera, Chalcididae: a new parasitoid of Historis odius (Fabricius (Lepidoptera, Nymphalidae

    Directory of Open Access Journals (Sweden)

    Hélcio R. Gil-Santana

    2005-12-01

    Full Text Available The first record of parasitism of Brachymeria pandora (Crawford, 1914 (Hymenoptera, Chalcididae on Historis odius (Fabricius, 1775 (Lepidoptera, Nymphalidae in the State of Rio de Janeiro, Brazil is presented.Apresenta-se o primeiro registro de parasitismo de Brachymeria pandora (Crawford, 1914 (Hymenoptera, Chalcididae em Historis odius (Fabricius, 1775 (Lepidoptera, Nymphalidae, no estado do Rio de Janeiro, Brasil.

  13. KEANEKARAGAMAN HYMENOPTERA PARASITIKA PADA TIPE EKOSISTEM BERBEDA DI BANGKA TENGAH, KEPULAUAN BANGKA BELITUNG

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    Herry Marta Saputra

    2017-05-01

    Full Text Available Diversity of parasitic Hymenoptera in different ecosystem types in Central Bangka, Bangka-Belitung Islands. Hymenoptera richness is dominated by parasitic species. More than 80% of Hymenoptera play a role as parasitoid on arthropods that are mostly insects. Diversity of parasitic Hymenoptera is widely studied in various types of terrestrial ecosystems including agro-ecosystem and non-agro-ecosystem. This study aimed to invent and compare the diversity of parasitic Hymenoptera in three different ecosystems, i.e., forest, oil palm plantation, and ex-tin mining. The location was located in Central Bangka Regency, Bangka Island. The study was conducted in Juli 2014 until October 2015. Parasitic Hymenoptera was collected with insect sweep net and yellow pan trap on one transect line with 1000 m length. Parasitic Hymenoptera were found on forest as much as 732 morphospecies, 326 morphospecies on oil palm plantations, and 293 morphospecies on ex-tin mining. Diversity and abundance of parasitic Hymenoptera on forest was higher than oil palm plantation and ex-tin mining area. Braconidae family was found dominant on forest, however on oil palm plantation and extin mining area the dominant family was Scelionidae.

  14. Four newly recorded species of the genus Meteorus Haliday (Hymenoptera: Braconidae: Euphorinae) from Korea

    OpenAIRE

    Hye-Rin Lee; Tae-Ho An; Deok-Seo Ku; Bong-Kyu Byun

    2017-01-01

    Four species of the genus Meteorus (Hymenoptera: Braconidae: Euphorinae) are reported for the first time from Korea: Meteorus brevicauda Thomson, 1895, Meteorus colon Haliday, 1835, Meteorus kunashiricus Belokobylskij, 1995, and Meteorus vexator Haliday, 1835. Diagnosis, distribution, and host information are provided for each species. Keywords: Braconidae, Hymenoptera, Korea, Meteorus, New record

  15. Four newly recorded species of the genus Meteorus Haliday (Hymenoptera: Braconidae: Euphorinae from Korea

    Directory of Open Access Journals (Sweden)

    Hye-Rin Lee

    2017-12-01

    Full Text Available Four species of the genus Meteorus (Hymenoptera: Braconidae: Euphorinae are reported for the first time from Korea: Meteorus brevicauda Thomson, 1895, Meteorus colon Haliday, 1835, Meteorus kunashiricus Belokobylskij, 1995, and Meteorus vexator Haliday, 1835. Diagnosis, distribution, and host information are provided for each species. Keywords: Braconidae, Hymenoptera, Korea, Meteorus, New record

  16. A Study of Allelopathy of Some Shade Trees to Coffea arabicaL. Seedlings

    Directory of Open Access Journals (Sweden)

    Adi Prawoto

    2006-05-01

    recommended as shade trees or intercrops with Arabica coffee and for D. zibethinusits cropping pattern must be arranged so the mineral competition could be maintained minimum. Key words: Allelopathy, Coffea arabica, Macadamia integrifolia, Cinnamomum burmani, Cassia siamea, Cassia spectabilis, mineral.

  17. Physiological Dose-Response of Coffee (Coffea arabica L. Plants to Glyphosate Depends on Growth Stage Respuesta Fisiológica de Plantas de Café (Coffea arabica L. a Glifosato Depende de la Etapa de Crecimiento

    Directory of Open Access Journals (Sweden)

    Leonardo Bianco de Carvalho

    2012-06-01

    Full Text Available Glyphosate is the main herbicide used in coffee (Coffea arabica L. plantations in Brazil. Problems with herbicide drift commonly occur in orchard fields due to non-adequate spraying conditions. A series of experiments was carried out aiming to evaluate physiological dose-response of C. arabica plants submitted to exposure to simulated glyphosate drift in two distinct plant growth stages. Glyphosate was applied at 0, 180, 360, and 720 g acid equivalent (AE ha-1 directly on coffee plants with 10 and 45 d after transplanting (DAT. Glyphosate doses in a range of 180-360 g AE ha-1 increased photosynthesis, transpiration and stomatal conductance in 10 DAT-plants up to 14 d after application (DAA while, in 45 DAT-plants, an increase was observed only up to 2 DAA, but this pattern was not persistent afterwards so that no difference in gas exchange was observed at 60 DAA in both plants. Macronutrient content was not affected by glyphosate application in both plants. Plant DM accumulation was not affected by glyphosate application at 10 DAT-plants, but an increase in plant growth was observed when glyphosate was applied in a range of 360-720 g AE ha-1 in 45 DAT-plants. Coffea arabica cv. Catuaí Vermelho IAC-144 responded differentially to glyphosate drift depending on plant growth stage, regarding on photosynthesis, transpiration, stomatal conductance, and plant growth, in spite of macronutrient nutrition was not affected.Glifosato es el principal herbicida utilizado en las plantaciones de café (Coffea arabica L. en Brasil. Problemas con la deriva de herbicidas comúnmente ocurren en los campos de cultivo debido a condiciones no adecuadas de pulverización. Una serie de experimentos se llevó a cabo con el objetivo de evaluar la relación dosis-respuesta fisiológica de plantas de C. arabica expuestas a situaciones simuladas de exposición a deriva de glifosato en dos etapas distintas de crecimiento de las plantas. El glifosato se aplicó en dosis de 0

  18. Taxonomia de Coffea arabica L. VI: caracteres morfológicos dos haplóides

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    A. Carvalho

    1952-06-01

    Full Text Available No decorrer dos trabalhos de melhoramento do café em execução no Instituto Agronômico de Campinas, plantas haplóides foram encontradas nas variedades typica, bourbon, maragogipe, semperflorens, laurina, erecta, caturra e San Ramon, da espécie Coffea arabica. Todos os haplóides apresentam porte menor e fôlhas mais estreitas e mais finas do que as variedades que lhes deram origem. Apesar de as flores serem completas, nota-se esterilidade muito acentuada. Raramente se formam alguns frutos, e êstes são providos de uma única semente, motivo pelo qual as plantas haplóides são denominadas "monosperma". Os fatôres genéticos dominantes maragogipe (Mg, erecta (Er, caturra (Ct e San Ramon, bem como os gens recessivos semperflorens (sf, e laurina (lr, das variedades estudadas, manifestam-se nas plantas haplóides, de modo semelhante ao que ocorre nas plantas diplóides correspondentes. O fator para coloração bronze dos brotos novos tem dominância incompleta e, na condição heterozigota (Brbr, mostra intensidade intermediária de côr. Nas plantas haplóides contendo um só alelo Br, a côr dos brotos novos é bronze-clara. Uma única dose do fator Na, que também apresenta dominância incompleta, dando, na forma heterozigota (Nana e na presença de tt, o fenótipo conhecido por murta, manifesta-se, na forma haplóide, dando plantas semelhantes às homozigotas ttNaNa, apenas com fôlhas mais estreitas e mais finas. Chamou-se atenção para as linhas puras de café obtidas pela duplicação do número de cromosômios dos haplóides e sua aplicação nos ensaios de linhagens e na determinação das taxas de mutação. As observações realizadas na meiose da espécie C. arabica, bem como os dados das análises genéticas e as observações feitas nesses haplóides parecem indicar que, se essa espécie fôr alotetraplóide, tal origem deve ser bem antiga, comportando-se hoje a espécie C. arábica, como um diplóide normal. As plantas

  19. Rust and Thinning Management Effect on Cup Quality and Plant Performance for Two Cultivars of Coffea arabica L.

    Science.gov (United States)

    Echeverria-Beirute, Fabian; Murray, Seth C; Klein, Patricia; Kerth, Chris; Miller, Rhonda; Bertrand, Benoit

    2018-05-30

    Beverage quality is a complex attribute of coffee ( Coffea arabica L.). Genotype (G), environment (E), management (M), postharvest processing, and roasting are all involved. However, little is known about how G × M interactions influence beverage quality. We investigated how yield and coffee leaf rust (CLR) disease (caused by Hemileia vastatrix Berk. et Br.) management affect cup quality and plant performance, in two coffee cultivars. Sensory and chemical analyses revealed that 10 of 70 attributes and 18 of 154 chemical volatile compounds were significantly affected by G and M. Remarkably, acetaminophen was found for the first time in roasted coffee and in higher concentrations under more stressful conditions. A principal component analysis described 87% of the variation in quality and plant overall performance. This study is a first step in understanding the complexity of the physiological, metabolic, and molecular changes in coffee production, which will be useful for the improvement of coffee cultivars.

  20. Allergen-specific immunotherapy of Hymenoptera venom allergy

    DEFF Research Database (Denmark)

    Schiener, Maximilian; Graessel, Anke; Ollert, Markus

    2017-01-01

    Stings of hymenoptera can induce IgE-mediated hypersensitivity reactions in venom-allergic patients, ranging from local up to severe systemic reactions and even fatal anaphylaxis. Allergic patients' quality of life can be mainly improved by altering their immune response to tolerate the venoms...... by injecting increasing venom doses over years. This venom-specific immunotherapy is highly effective and well tolerated. However, component-resolved information about the venoms has increased in the last years. This knowledge is not only able to improve diagnostics as basis for an accurate therapy......, but was additionally used to create tools which enable the analysis of therapeutic venom extracts on a molecular level. Therefore, during the last decade the detailed knowledge of the allergen composition of hymenoptera venoms has substantially improved diagnosis and therapy of venom allergy. This review focuses...

  1. História natural da interação entre Meteorus sp. Haliday, 1835 (Hymenoptera: Braconidae) e seu hiperparasitoide Toxeumella albipes Girault, 1913 (Hymenoptera: Pteromalidae)

    OpenAIRE

    Sobczak, JF; Maia, DP; Moura, JCMS; Costa, VA; Vasconcellos-Neto, J

    2012-01-01

    Some parasitoids build a cocoon mass that hangs in the host body until the adults emergence, which is an advantage against attack by predators who troll the vegetation in search of prey. However, such behaviour is not effective against the hyperparasitoid attacks. This study reports the interaction between the caterpillar Manduca sexta Linnaeus, 1763 (Lepidoptera, Sphingidae) parasitised by Meteorus sp. (Hymenoptera, Braconidae) larvae and its hyperparasitoid Toxeumella albipes (Hymenoptera, ...

  2. On the identity of Melipona torrida Friese (Hymenoptera, Apidae)

    OpenAIRE

    Melo, Gabriel A. R.

    2013-01-01

    On the identity of Melipona torrida Friese (Hymenoptera, Apidae). Melipona marginata var. torrida Friese, 1916, described from three workers putatively collected in Costa Rica, never had its identity properly recognized. Since its original description, no additional specimens have ever been collected in Costa Rica. It is argued here that Melipona torrida was based on mislabeled specimens and corresponds to Melipona marginata obscurior Moure, 1971, a form known only from southern Brazil, Argen...

  3. Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)

    Czech Academy of Sciences Publication Activity Database

    Carabajal Paladino, Leonela Z.; Lo Nostro, F.; Papeschi, A.; Cladera, J.; Bressa, M. J.

    2017-01-01

    Roč. 98, č. 1 (2017), s. 38-43 ISSN 0001-7272 R&D Projects: GA MŠk(CZ) EE2.3.30.0032 Institutional support: RVO:60077344 Keywords : Hymenoptera * modified meiosis * abortive division Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Genetics and heredity (medical genetics to be 3) Impact factor: 1.211, year: 2016 http://onlinelibrary.wiley.com/doi/10.1111/azo.12148/pdf

  4. Cross-reacting carbohydrate determinants and hymenoptera venom allergy.

    Science.gov (United States)

    Brehler, Randolf; Grundmann, Sonja; Stöcker, Benedikt

    2013-08-01

    Insect venom allergy is an important cause of anaphylaxis. Venom immunotherapy assume the clear identification of the culprit insect, but this is impeded by Immunoglobulin E (IgE) antibodies to cross reactive carbohydrate determinant (CCD) epitopes of common glycoproteins. Here we give an overview about inducers, importance, and relevance of anti-N-Glycan CCD IgE antibodies. Pollen exposure and insect stings induce anti-CCD IgE antibodies interfering with in-vitro tests for allergy diagnosis due to extensive IgE cross-reactivity. Instead of being biologically active these antibodies are irrelevant for allergic reactions due to hymenoptera stings. The general response of the immune system to the ubiquitous exposure to N-glycan containing glycoproteins is still a matter of debate. CCD specific IgG antibodies in sera of bee keepers suggest tolerance induction due to high-dose exposure. Tolerance induction by pollen and food glycoproteins has not been proved. Hymenoptera stings and pollen exposure induce anti-CCD IgE. In regard to anaphylaxis due to Hymenoptera stings these antibodies are not clinically relevant, but they are important for the specificity of in-vitro tests proving insect venom allergy. The introduction of component based diagnostic IgE testing improves the specificity of in-vitro tests if proteins devoid of CCD epitopes are used.

  5. Arbuscular mycorrhizal fungi associated with shade trees and Coffea arabica L. in a coffee-based agroforestry system in Bonga, Southwestern Ethiopia

    OpenAIRE

    Sewnet ,Tadesse Chanie; Tuju, Fassil Assefa

    2013-01-01

    In a first step to understand the interactions between Coffea arabica L. trees and mycorrhizae in Ethio¬pia, an investigation of the current mycorrhizal colonization status of roots was undertaken. We sampled 14 shade tree species occurring in coffee populations in Bonga forest, Ethiopia. Milletia fer¬ruginea, Schefflera abyssinica, Croton macrostachyus, Ficus vasta, F. sur, Albizia gummifera, Olea capensis, Cordia africana, Ehretia abyssinica, Pouteria adolfi-friederici, Pavetta oliveriana, ...

  6. Cloning, expression, crystallization and preliminary X-ray analysis of the XMT and DXMT N-methyltransferases from Coffea canephora (robusta)

    Energy Technology Data Exchange (ETDEWEB)

    McCarthy, Andrew A., E-mail: andrewmc@embl.fr [European Molecular Biology Laboratory, 6 Rue Jules Horowitz, BP 181, 38042 Grenoble (France); Biget, Laurent [Nestlé Research and Development, 101 Avenue Gustave Eiffel, Notre-Dame D’Oe, 37097 Tours (France); Lin, Chenwei [Department of Plant Breeding and Genetics, Department of Plant Biology, Cornell University, Ithaca, NY 14853 (United States); Petiard, Vincent [Nestlé Research and Development, 101 Avenue Gustave Eiffel, Notre-Dame D’Oe, 37097 Tours (France); Tanksley, Steve D. [Department of Plant Breeding and Genetics, Department of Plant Biology, Cornell University, Ithaca, NY 14853 (United States); McCarthy, James G. [Nestlé Research and Development, 101 Avenue Gustave Eiffel, Notre-Dame D’Oe, 37097 Tours (France); European Molecular Biology Laboratory, 6 Rue Jules Horowitz, BP 181, 38042 Grenoble (France)

    2007-04-01

    The genes encoding XMT and DXMT, the enzymes from Coffea canephora (robusta) that catalyse the three independent N-methyl transfer reactions in the caffeine-biosynthesis pathway, have been cloned and the proteins have been expressed in Escherichia coli. Both proteins have been crystallized in the presence of the demethylated cofactor S-adenosyl-l-cysteine (SAH) and substrate (xanthosine for XMT and theobromine for DXMT). Caffeine is a secondary metabolite produced by a variety of plants including Coffea canephora (robusta) and there is growing evidence that caffeine is part of a chemical defence strategy protecting young leaves and seeds from potential predators. The genes encoding XMT and DXMT, the enzymes from Coffea canephora (robusta) that catalyse the three independent N-methyl transfer reactions in the caffeine-biosynthesis pathway, have been cloned and the proteins have been expressed in Escherichia coli. Both proteins have been crystallized in the presence of the demethylated cofactor S-adenosyl-l-cysteine (SAH) and substrate (xanthosine for XMT and theobromine for DXMT). The crystals are orthorhombic, with space group P2{sub 1}2{sub 1}2{sub 1} for XMT and C222{sub 1} for DXMT. X-ray diffraction to 2.8 Å for XMT and to 2.5 Å for DXMT have been collected on beamline ID23-1 at the ESRF.

  7. Cloning, expression, crystallization and preliminary X-ray analysis of the XMT and DXMT N-methyltransferases from Coffea canephora (robusta)

    International Nuclear Information System (INIS)

    McCarthy, Andrew A.; Biget, Laurent; Lin, Chenwei; Petiard, Vincent; Tanksley, Steve D.; McCarthy, James G.

    2007-01-01

    The genes encoding XMT and DXMT, the enzymes from Coffea canephora (robusta) that catalyse the three independent N-methyl transfer reactions in the caffeine-biosynthesis pathway, have been cloned and the proteins have been expressed in Escherichia coli. Both proteins have been crystallized in the presence of the demethylated cofactor S-adenosyl-l-cysteine (SAH) and substrate (xanthosine for XMT and theobromine for DXMT). Caffeine is a secondary metabolite produced by a variety of plants including Coffea canephora (robusta) and there is growing evidence that caffeine is part of a chemical defence strategy protecting young leaves and seeds from potential predators. The genes encoding XMT and DXMT, the enzymes from Coffea canephora (robusta) that catalyse the three independent N-methyl transfer reactions in the caffeine-biosynthesis pathway, have been cloned and the proteins have been expressed in Escherichia coli. Both proteins have been crystallized in the presence of the demethylated cofactor S-adenosyl-l-cysteine (SAH) and substrate (xanthosine for XMT and theobromine for DXMT). The crystals are orthorhombic, with space group P2 1 2 1 2 1 for XMT and C222 1 for DXMT. X-ray diffraction to 2.8 Å for XMT and to 2.5 Å for DXMT have been collected on beamline ID23-1 at the ESRF

  8. Parasitismo entre especies (Diptera, Hymenoptera en los nidos de Stictia signata (Hymenoptera: Sphecidae

    Directory of Open Access Journals (Sweden)

    Julio A. Genaro

    1999-09-01

    Full Text Available S. signata es una de las avispas de la arena más frecuentemente observada en los cayos y las costas de Cuba. Las hembras construyen los nidos en la arena y los abastecen con moscas, para alimentar a la descendencia. Se describe la conducta de dos especies: Liohippelates n. sp. circa collusor (Diptera: Chloropidae y Hexacola sp. (Hymenoptera: Eucoilidae para penetrar al interior de los nidos de S. signata. Las observaciones se efectuaron durante 1989 hasta 1991, en playa Caimito, sur de la provincia de La Habana, Cuba. Liohippelates cleptoparasitó el 100% de los nidos. Sus larvas necrófagas se alimentaron de los restos de las presas dejadas por la larva de S. signata, sin afectarla. Sólo en un caso la larva mostró signos de mortalidad, porque además del número alto de cleptoparásitos inmaduros, habían 53 moscas adultas alimentándose de los fluidos corporales de las presas. Hexacola sp. fue un parasitoide de las larvas de Liohippelates, en el interior de las celdillas. A pesar del elevado cleptoparasitismo, la población del esfécido se mantuvo elevada durante los años de observación.Stictia signata is one of the most frequently observed sand wasps in the Cuban keys and coasts. Females build their nests in the sand and supply them with flies to feed offspring. Here, I describe the behavior of two species, Liohippelates n. sp. near collusor (Diptera: Chloropidae and Hexacola sp. (Hymenoptera: Eucoilidae, which enter the nests of S. signata. The observations were carried out from 1989 through 1991 in Caimito beach, Southern Havana province, Cuba. Liohippelates inhabited 100% of the nests. Its necrofagous larvae fed on the remnants of prey left by the larva of S. signata, without affecting the larva. Only in one case did the larva show signs of mortality because, apart from the high number of immature cleptoparasites, there were 53 adult flies feeding on prey body fluids. Hexacola sp. parasitized the larvae of Liohippelates within the

  9. Abridged life tables for Cephalonomia stephanoderis and Prorops nasuta (Hymenoptera: Bethylidae) Parasitoids of Hypothenemus hampei (Coleoptera: Scolytidae) reared on artificial diet

    Science.gov (United States)

    Biological aspects and demographic parameters of Cephalonomia stephanoderis Betrem (Hymenoptera: Bethylidae) and Prorops nasuta Waterston (Hymenoptera: Bethylidae) parasitoids of the coffee berry borer (CBB), Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) were investigated usi...

  10. Meta-analysis for heritability estimates of vegetative and reproductive traits of Coffea arabica L.Meta-análise para estimativas de herdabilidade de caracteres vegetativos e reprodutivos de Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    Danielle Pereira Baliza

    2012-08-01

    Full Text Available The compilation of informations resulting from independent studies has been difficulted in almost all scientific fields, mainly due to the great number of scientific papers published in recent years. As a result, summarizing information became a need. In this context, a meta-analysis was conducted with the objective of summarizing the estimates for the heritability for vegetative and reproductive traits of coffee (Coffea arabica L.. Heritability estimates were appraised regarding the following traits: average height of plant, average diameter of the canopy, vegetative vigor, production of processed coffee, yield and rust. The data regarding the heritability estimates are from scientific articles published in national and international journals, congress annals, PhD thesis and Master dissertations. The technique of meta-analysis summarized the estimates heritability from different studies and made possible to conclude that all of the appraised traits are highly inherited, reflecting the great genetic variety of coffee plants, and that is possible to reach satisfactory genetic gains in improvement programs in which those traits are evaluated. A compilação de informações advindas de estudos independentes tem sido dificultada em quase todos os campos da ciência, devido principalmente, ao grande número de trabalhos científicos publicados nos últimos anos. Assim, sumarizar informações tornou-se uma necessidade. Neste contexto, uma meta-análise foi conduzida com o objetivo de sistematizar as estimativas para a herdabilidade de caracteres vegetativos e reprodutivos de cafeeiros (Coffea arabica L.. Foram avaliadas as estimativas de herdabilidade referentes aos seguintes caracteres: altura média da planta, diâmetro médio da copa, vigor vegetativo, produção de café beneficiado, rendimento e resistência a ferrugem. Os dados referentes às estimativas de herdabilidade são provenientes de artigos científicos publicados em revistas

  11. Perfil sensorial da bebida café (Coffea arabica L. determinado por análise tempo-intensidade Sensorial profile of beverage coffee (Coffea arabica L. determined by analysis time-intensity

    Directory of Open Access Journals (Sweden)

    Marlene A. M. Monteiro

    2005-12-01

    Full Text Available O presente trabalho teve como objetivo avaliar o gosto amargo, sabor fermentado e sabor queimado de nove amostras (mole/clara, dura/clara, rio/clara, mole/expresso, dura/expresso, rio/expresso, mole/escura, dura/escura e rio/escura da bebida café (Coffea arabica L. por meio da análise Tempo-Intensidade (TI. Foram avaliados seis parâmetros da curva: tempo para atingir a intensidade máxima (TImáx, intensidade máxima do estímulo (Imáx, tempo correspondente ao ponto onde a intensidade máxima começa a declinar (Td, tempo de duração da intensidade máxima (Platô, área sob a curva (Área e tempo total de duração do estímulo (Ttot. A análise demonstrou que as amostras de torra escura tiveram maior intensidade máxima (Imáx e tempo de duração do estímulo (Ttot para gosto amargo e sabor queimado, sendo que as amostras de torra clara apresentaram menor intensidade destes estímulos. Em relação ao sabor fermentado, a amostra rio/expresso foi a que apresentou maior intensidade.The present work had as objective to evaluate the bitter taste, fermented flavor and burned flavor of nine samples (soft/light, hard/light, rio/light, soft/express, hard/express, rio/express, soft/dark, hard/dark and rio/dark of the drink coffee (Coffea arabica L. through the analysis Time-Intensity (TI. Six parameters of the curve were evaluated: time to reach the maximum intensity (TImáx, maximum intensity of the incentive (Imáx, time corresponding to the point where the maximum intensity begins to refuse (Td, time of duration of the maximum intensity (Plateau, area under the curve (Área and total time of duration of the incentive (Ttot. The analysis demonstrated that the samples of dark toast had larger maximum intensity (Imáx and time of duration of the incentive (Ttot for bitter taste and burned flavor, while the samples of light toast presented smaller intensity of these stimulus. For the fermented flavor, the sample rio/express was the one that

  12. A NEW SPECIES OF INVASIVE GALL WASP (HYMENOPTERA: EULOPHIDAE: TETRASTICHINAE) ON BLUE GUM (EUCALYPTUS GLOBULUS) IN CALIFORNIA

    Science.gov (United States)

    The blue gum gall wasp, Selitrichodes globulus La Salle & Gates (Hymenoptera: Eulophidae: Tetrastichinae), is described as an invasive gall inducer on blue gum, Eucalyptus globulus (Myrtaceae), in California....

  13. A new species of Chelonus (Areselonus) (Hymenoptera, Braconidae) from India reared from Acrocercops lysibathra (Meyrick) on Cordia latifolia Roxb.

    OpenAIRE

    Ahmad, Zubair; Ghramh, Hamed A.

    2018-01-01

    Chelonus (Areselonus) spinigaster sp. n., (Hymenoptera: Braconidae: Cheloninae) is described from India. The new species was reared from the moth species Acrocercops lysibathra (Meyr.) on Cordia latifolia Roxb.

  14. A new species of Chelonus (Areselonus) (Hymenoptera, Braconidae) from India reared from Acrocercops lysibathra (Meyrick) on Cordia latifolia Roxb.

    Science.gov (United States)

    Ahmad, Zubair; Ghramh, Hamed A

    2018-01-01

    Chelonus (Areselonus) spinigaster sp. n. , (Hymenoptera: Braconidae: Cheloninae) is described from India. The new species was reared from the moth species Acrocercops lysibathra (Meyr.) on Cordia latifolia Roxb.

  15. Trophobiosis in the arboricolous ant .i.Liometopum microcephalum./i. (Hymenoptera: Formicidae: Dolichoderinae)

    Czech Academy of Sciences Publication Activity Database

    Schlaghamerský, J.; Kašpar, J.; Petráková, L.; Šustr, Vladimír

    2013-01-01

    Roč. 110, č. 2 (2013), s. 231-239 ISSN 1210-5759 Institutional support: RVO:60077344 Keywords : Hymenoptera * arboricolous * ants * trophobiosis Subject RIV: EG - Zoology Impact factor: 1.076, year: 2013

  16. Two new species of the genus Peristenus Foerster (Hymenoptera: Braconidae: Euphorinae) from the Canary Islands

    NARCIS (Netherlands)

    Achterberg, van C.; Guerrero, E.R.

    2003-01-01

    Two new species of the genus Peristenus Foerster (Hymenoptera: Braconidae: Euphorinae) from the Canary Islands are described and illustrated: Peristenus angifemoralis spec. nov. from Tenerife, and P. gloriae spec. nov. from Gran Canaria and Tenerife.

  17. Natural history of Hymenoptera venom allergy in Eastern Spain.

    Science.gov (United States)

    Fernandez, J; Soriano, V; Mayorga, L; Mayor, M

    2005-02-01

    The natural history of stings, the clinical reaction of the patient and in vivo and in vitro tests are necessary parameters to assess before initiating Hymenoptera venom immunotherapy. In the decision to initiate immunotherapy with Hymenoptera venom, it is not usual to evaluate the natural history of the disease, which seems to be self-limiting and therefore of variable clinical significance. Our aim was to determine the natural history of Hymenoptera hypersensitivity over 4 consecutive years in a rural Mediterranean population. An epidemiological study of Hymenoptera sting reactions and possible sensitivity was carried out in 145 randomly selected subjects out of a rural Mediterranean population of 600. Seventy-two subjects, including those with a history of anaphylaxis, completed the 4-year study. The nature of their clinical reactions, age, sex, history of atopy, profession, family history of reactions to Hymenoptera insects, time elapsed since the last sting, number of stings and specific IgE and IgG were determined (the latter, to the three most important insects in the area: Apis mellifera, Polistes dominulus, and Vespula germanica). Of the 72 subjects, four subjects had systemic reactions (SR), 23 had large local reaction (LLR) and all the others (117) was minor local reactions. None who had experienced an SR had a repeat SR when re-stung over the 4-year study. Of those with LLR, 12 subjects had the same type of reaction and 11 experienced more mild local reactions when re-stung. In the SR and local reaction groups, IgE to honey bee (Hb) increased significantly during the study period, whereas in those with only LLR, specific IgE to wasp (Polistes) decreased. Specific IgG to Polistes and Vespula (wasps) decreased significantly, whereas there was no change in the specific IgG to Hb in any of the groups. The number of stings per year decreased at the end of the study in all groups, but positive-specific IgG was higher in subjects with the greatest number of

  18. A flórula invasora da cultura do café (Coffea arabica L. no Estado de Minas Gerais, Brasil Weeds in coffee (Coffea arabica L. plantations in the state of Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Manuel Losada Gavilanes

    1988-01-01

    Full Text Available Nas áreas de cultura de café (Coffea arábica L., no Estado de Minas Gerais, foram coletadas e identificadas 388 espécies de plantas invasoras (= plantas daninhas, pertencentes a 51 famílias botânicas, representando 182 gêneros, sendo que as famílias Compositae, Gramineae, Leguminosae, Malvaceae, Solanaceae, Euphorbiaceae, Rubiaceae, Amaranthaceae, Convolvulaceae e Verbenaceae, são as mais importantes em relação à cultura. As plantas coletadas, devidamente etiquetadas e identificadas, foram anexadas, parte delas no PAMG (Herbário da EPAMIG, Belo Horizonte, MG e, a outra parte, no Herbarium ESAL (Herbário do Departamento de Biologia da Escola Superior de Agricultura de Lavras - ESAL, Lavras - MG.A survey in the cultivation area of coffee in the State of Minas Gerais, Brazil, has resulted in the determination of 388 weed species, of 182 genera belonging to 51 families; the families presenting a greater number of espécies are: Compositae, Leguminosae, Gramineae, Malvaceae, Solanaceae, Rubiaceae, Convolvulaceae, Euphorbiaceae, Amaranthaceae and Verbenaceae with 65, 48, 42, 30, 19, 17, 16, 14, 12, 10 species, respectively.

  19. Spatial Analysis of Agricultural Landscape and Hymenoptera Biodiversity at Cianjur Watershed

    Directory of Open Access Journals (Sweden)

    YAHERWANDI

    2006-12-01

    Full Text Available Hymenoptera is one of the four largest insect order (the other three are Coleoptera, Diptera, and Lepidoptera. There are curerently over 115 000 described Hymenoptera species. It is clear that Hymenoptera is one of the major components of insect biodiversity. However, Hymenoptera biodiversity is affected by ecology, environment, and ecosystem management. In an agricultural areas, the spatial structure, habitat diversity, and habitat composition may vary from cleared landscapes to structurally rich landscape. Thus, it is very likely that such large-scale spatial patterns (landscape effects may influence local biodiversity and ecological functions. Therefore, the objective of this research were to study diversity and configuration elements of agricultural landscapes at Cianjur Watershed with geographical information sytems (GIS and its influence on Hymenoptera biodiversity. The structural differences between agricultural landscapes of Nyalindung, Gasol, and Selajambe were characterized by patch analyst with ArcView 3.2 of digital land use data. Results indicated that class of land uses of Cianjur Watershed landscape were housing, mixed gardens, talun and rice, vegetable, and corn fields. Landscape structure influenced the biodiversity of Hymenoptera. Species richness and the species diversity were higher in Nyalindung landscape compare to Gasol and Selajambe landscape.

  20. Pengaruh Habitat Sekitar Lahan Persawahan dan Umur Tanaman Padi terhadap Keanekaragaman Hymenoptera Parasitika

    Directory of Open Access Journals (Sweden)

    Nina Herlina

    2015-09-01

    Full Text Available As the largest group of biological control agents, Parasitic Hymenoptera play important role in controlling pest outbreak in agricultural habitat. Unfortunately, there is lack of information about how these parasitoids occur in agricultural habitat related to condition of surrounding habitat and phenology of crop plant. The objective of this research was to study the effect of rice field surrounding habitat and age of rice plant on the diversity of Parasitic Hymenoptera. Research area was located in Carang Pulang Village, Dramaga, Bogor. We selected four blocks which represented different of habitat condition and age of rice plant. Each block was set six yellow pan traps (with minimum distance 20 meter and one malaise trap. Sampling of insects were conducted weekly from 6 to 12 week after planting. From this research, we collected 1,833 individual of Hymenoptera (without ants belong to 9 superfamilies, 23 families, and 216 species. Parasitic Hymenoptera was more abundant (96% and species rich (84% than Aculeata. Rice field surrounding habitat (block and age of rice field significantly affect the diversity of Parasitic Hymenoptera. We found positively correlation between age of rice plant and species richness of Parasitic Hymenoptera.

  1. Taxonomic and functional responses to fire and post-fire management of a Mediterranean hymenoptera community.

    Science.gov (United States)

    Mateos, Eduardo; Santos, Xavier; Pujade-Villar, Juli

    2011-11-01

    Fire is one of the commonest disturbances worldwide, transforming habitat structure and affecting ecosystem functioning. Understanding how species respond to such environmental disturbances is a major conservation goal that should be monitored using functionally and taxonomically diverse groups such as Hymenoptera. In this respect, we have analyzed the taxonomic and functional response to fire and post-fire management of a Hymenoptera community from a Mediterranean protected area. Thus, Hymenoptera were sampled at fifteen sites located in three burnt areas submitted to different post-fire practices, as well as at five sites located in peripheral unburnt pine forest. A total of 4882 specimens belonging to 33 families, which were classified into six feeding groups according to their dietary preferences, were collected. ANOVA and Redundancy Analyses showed a taxonomic and functional response to fire as all burnt areas had more Hymenoptera families, different community composition and higher numbers of parasitoids than the unburnt area. Taxonomic differences were also found between burnt areas in terms of the response of Hymenoptera to post-fire management. In general the number of parasitoids was positively correlated to the number of potential host arthropods. Parasitoids are recognized to be sensitive to habitat changes, thus highlighting their value for monitoring the functional responses of organisms to habitat disturbance. The taxonomic and functional responses of Hymenoptera suggest that some pine-forest fires can enhance habitat heterogeneity and arthropod diversity, hence increasing interspecific interactions such as those established by parasitoids and their hosts.

  2. Taxonomic and Functional Responses to Fire and Post-Fire Management of a Mediterranean Hymenoptera Community

    Science.gov (United States)

    Mateos, Eduardo; Santos, Xavier; Pujade-Villar, Juli

    2011-11-01

    Fire is one of the commonest disturbances worldwide, transforming habitat structure and affecting ecosystem functioning. Understanding how species respond to such environmental disturbances is a major conservation goal that should be monitored using functionally and taxonomically diverse groups such as Hymenoptera. In this respect, we have analyzed the taxonomic and functional response to fire and post-fire management of a Hymenoptera community from a Mediterranean protected area. Thus, Hymenoptera were sampled at fifteen sites located in three burnt areas submitted to different post-fire practices, as well as at five sites located in peripheral unburnt pine forest. A total of 4882 specimens belonging to 33 families, which were classified into six feeding groups according to their dietary preferences, were collected. ANOVA and Redundancy Analyses showed a taxonomic and functional response to fire as all burnt areas had more Hymenoptera families, different community composition and higher numbers of parasitoids than the unburnt area. Taxonomic differences were also found between burnt areas in terms of the response of Hymenoptera to post-fire management. In general the number of parasitoids was positively correlated to the number of potential host arthropods. Parasitoids are recognized to be sensitive to habitat changes, thus highlighting their value for monitoring the functional responses of organisms to habitat disturbance. The taxonomic and functional responses of Hymenoptera suggest that some pine-forest fires can enhance habitat heterogeneity and arthropod diversity, hence increasing interspecific interactions such as those established by parasitoids and their hosts.

  3. Estimating biophysical properties of coffee (Coffea canephora) plants with above-canopy field measurements, using CropSpec®

    Science.gov (United States)

    Putra, Bayu T. Widjaja; Soni, Peeyush; Morimoto, Eiji; Pujiyanto, Pujiyanto

    2018-04-01

    Remote sensing technologies have been applied to many crops, but tree crops like Robusta coffee (Coffea canephora) under shade conditions require additional attention while making above-canopy measurements. The objective of this study was to determine how well chlorophyll and nitrogen status of Robusta coffee plants can be estimated with the laser-based (CropSpec®) active sensor. This study also identified appropriate vegetation indices for estimating Nitrogen content by above-canopy measurement, using near-infra red and red-edge bands. Varying light intensity and different background of the plants were considered in developing the indices. Field experiments were conducted involving different non-destructive tools (CropSpec® and SPAD-502 chlorophyll meter). Subsequently, Kjeldahl laboratory analyses were performed to determine the actual Nitrogen content of the plants with different ages and field conditions used in the non-destructive previous stage. Measurements were undertaken for assessing the biophysical properties of tree plant. The usefulness of near-infrared and red-edge bands from these sensors in measuring critical nitrogen levels of coffee plants by above-canopy measurement are investigated in this study.

  4. Developing core collections to optimize the management and the exploitation of diversity of the coffee Coffea canephora.

    Science.gov (United States)

    Leroy, Thierry; De Bellis, Fabien; Legnate, Hyacinthe; Musoli, Pascal; Kalonji, Adrien; Loor Solórzano, Rey Gastón; Cubry, Philippe

    2014-06-01

    The management of diversity for conservation and breeding is of great importance for all plant species and is particularly true in perennial species, such as the coffee Coffea canephora. This species exhibits a large genetic and phenotypic diversity with six different diversity groups. Large field collections are available in the Ivory Coast, Uganda and other Asian, American and African countries but are very expensive and time consuming to establish and maintain in large areas. We propose to improve coffee germplasm management through the construction of genetic core collections derived from a set of 565 accessions that are characterized with 13 microsatellite markers. Core collections of 12, 24 and 48 accessions were defined using two methods aimed to maximize the allelic diversity (Maximization strategy) or genetic distance (Maximum-Length Sub-Tree method). A composite core collection of 77 accessions is proposed for both objectives of an optimal management of diversity and breeding. This core collection presents a gene diversity value of 0.8 and exhibits the totality of the major alleles (i.e., 184) that are present in the initial set. The seven proposed core collections constitute a valuable tool for diversity management and a foundation for breeding programs. The use of these collections for collection management in research centers and breeding perspectives for coffee improvement are discussed.

  5. Ochratoxigenic fungi associated with green coffee beans (Coffea arabica L.) in conventional and organic cultivation in Brazil.

    Science.gov (United States)

    de Fátima Rezende, Elisângela; Borges, Josiane Gonçalves; Cirillo, Marcelo Ângelo; Prado, Guilherme; Paiva, Leandro Carlos; Batista, Luís Roberto

    2013-01-01

    The genera Aspergillus comprises species that produce mycotoxins such as aflatoxins, ochratoxins and patulin. These are cosmopolitan species, natural contaminants of agricultural products. In coffee grains, the most important Aspergillus species in terms of the risk of presenting mycotoxins belong to the genera Aspergillus Section Circumdati and Section Nigri. The purpose of this study was to assess the occurrence of isolated ochratoxigenic fungi of coffee grains from organic and conventional cultivation from the South of Minas Gerais, Brazil, as well as to evaluate which farming system presents higher contamination risk by ochratoxin A (OTA) produced by fungi. Thirty samples of coffee grains (Coffea arabica L.) were analysed, being 20 of them of conventional coffee grains and 10 of them organic. The microbiological analysis was done with the Direct Plating Technique in a Dichloran Rose Bengal Chloramphenicol Agar (DRBC) media. The identification was done based on the macro and micro morphological characteristics and on the toxigenic potential with the Plug Agar technique. From the 30 samples analysed, 480 filamentous fungi of the genera Aspergillus of the Circumdati and Nigri Sections were isolated. The ochratoxigenic species identified were: Aspergillus auricoumus, A. ochraceus, A. ostianus, A. niger and A. niger Aggregate. The most frequent species which produces ochratoxin A among the isolated ones was A. ochraceus, corresponding to 89.55%. There was no significant difference regarding the presence of ochratoxigenic A. ochreceus between the conventional and organic cultivation systems, which suggests that the contamination risk is similar for both cultivation systems.

  6. Inter-genomic DNA Exchanges and Homeologous Gene Silencing Shaped the Nascent Allopolyploid Coffee Genome (Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    Philippe Lashermes

    2016-09-01

    Full Text Available Allopolyploidization is a biological process that has played a major role in plant speciation and evolution. Genomic changes are common consequences of polyploidization, but their dynamics over time are still poorly understood. Coffea arabica, a recently formed allotetraploid, was chosen to study genetic changes that accompany allopolyploid formation. Both RNA-seq and DNA-seq data were generated from two genetically distant C. arabica accessions. Genomic structural variation was investigated using C. canephora, one of its diploid progenitors, as reference genome. The fate of 9047 duplicate homeologous genes was inferred and compared between the accessions. The pattern of SNP density along the reference genome was consistent with the allopolyploid structure. Large genomic duplications or deletions were not detected. Two homeologous copies were retained and expressed in 96% of the genes analyzed. Nevertheless, duplicated genes were found to be affected by various genomic changes leading to homeolog loss or silencing. Genetic and epigenetic changes were evidenced that could have played a major role in the stabilization of the unique ancestral allotetraploid and its subsequent diversification. While the early evolution of C. arabica mainly involved homeologous crossover exchanges, the later stage appears to have relied on more gradual evolution involving gene conversion and homeolog silencing.

  7. Revealing the Diversity of Introduced Coffea canephora Germplasm in Ecuador: Towards a National Strategy to Improve Robusta

    Directory of Open Access Journals (Sweden)

    Rey Gastón Loor Solórzano

    2017-01-01

    Full Text Available Genetic resources of Coffea canephora have been introduced in several tropical countries with potential for crop development. In Ecuador, the species has been cultivated since the mid-20th century. However, little is known about the diversity and genetic structure of introduced germplasm. This paper provides an overview of the genetic and phenotypic diversity of C. canephora in Ecuador and some proposals for implementing a breeding program. Twelve SSR markers were used to analyze 1491 plants of C. canephora grown in different living collections in Ecuador, compared to 29 genotypes representing the main genetic and geographic diversity groups identified within the species. Results indicated that most of the genotypes introduced are of Congolese origin, with accessions from both main subgroups, SG1 and SG2. Some genotypes were classed as hybrids between both subgroups. Substantial phenotypic diversity was also found, and correlations were observed with genetic diversity. Ecuadorian Robusta coffee displays wide genetic diversity and we propose some ways of improving Robusta in Ecuador. A breeding program could be based on three operations: the choice of elite clones, the introduction of new material from other countries (Ivory Coast, Uganda, and the creation of new hybrid material using genotypes from the different diversity groups.

  8. CARACTHERIZATION OF BIOMASS ENERGY AND CARBONIZATION OF COFFEE GRAINS (Coffea arabica, L AND (Cedrelinga catenaeformis, DUKE WOOD RESIDUES

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    Ailton Teixeira do Vale

    2007-12-01

    Full Text Available Brazil produces annually two million tons of coffee s husks from farms or industrial processing units. This wastematerial can be used for energy production; currently it is mainly used in agricultural practices as field straw cover up. This paperdeals with coffee s (Coffea arabica, L husks biomass energy characteristics, including wood carbonization. As a reference, the samestudy was performed with a wood species regularly used for building construction named Cedrorana (Cedrelinga catenaeformis,Duke. Coffee s husks was obtained from a farm 150 km far from Brasilia city and cedrorana sawdust from a local saw mill. Thispaper presents results from energy and biomass variables like moisture content, bulk density, lower and superior heating power, ashcontent, fixed carbon, volatile matter and volumetric energy. It has also studied carbonization, charcoal, pyroligneous licqor and noncondensablegases. A comparison between Coffee s husk with 0% moisture content and Cedrorana sawdust portrays the followingresults: bulk density 144.41 kg/m3, fixed carbon 10.31%, superior heating power 4.57 kWh (or 16.46 MJ or 3.933 Mcal/kg, charcoalcontent 40,64% and heating value per cubic meter 2,179 MJ/m3

  9. EFEITO DE Ilex paraguariensis St. Hil. e Coffea arabica L. SOBRE O CRESCIMENTO DE Fonsecaea pedrosoi ATCC 46428

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    Maria Lucia Scroferneker

    2007-12-01

    Full Text Available Este trabalho avaliou o efeito de extratos aquosos de Ilex paraguariensis (erva-mate e Coffea arabica (café em ágar Sabouraud dextrose no crescimento de Fonsecaea pedrosoi ATCC 46428. F. pedrosoi foi cultivada em placas de Petri contendo ágar Sabouraud dextrose suplementado com extratos aquosos derivados de 0,5; 1; 2; 3; 4 e 5g de pó de erva-mate ou de café fervidos em 100ml de água destilada por 30 min. Os diâmetros das colônias do fungo foram determinados após 7 dias. A incorporação dos extratos de erva-mate ou café no meio de crescimento não causou diferenças significativas no crescimento radial de F. pedrosoi ATCC 46428 comparado ao controle. Entretanto, estudos sobre o requerimento nutricional são importantes na sistematização do perfil bioquímico, o que pode contribuir na elucidação da bioquímica funcional do microrganismo.

  10. Observações anatômicas em plantas de Coffea arabica L. obtidas por enraizamento de estacas

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    Adriana Madeira Santos Jesus

    2010-04-01

    Full Text Available Uma forma para se obter diminuição significativa de tempo e recursos despendidos nos programas de melhoramento de Coffea arabica L. é a clonagem de híbridos F1 por meio de estacas caulinares. Alguns estudos, em diferentes instituições, foram realizados buscando-se definir um método eficiente para esse tipo de clonagem. Com o objetivo de verificar-se a presença de barreiras anatômicas ao enraizamento de estacas caulinares do cafeeiro e a origem das raízes adventícias, bem como compara-las às raízes provenientes de plantas obtidas por semeadura, foram realizadas análises anatômicas no Departamento de Biologia da Universidade Federal de Lavras (UFLA, Lavras, MG. Utilizaram-se estacas caulinares de cafeeiro dos cultivares Acaiá e Rubi e mudas obtidas por semeadura direta e por estaquia. Os cortes realizados nas estacas caulinares mostraram não existirem barreiras anatômicas ao enraizamento adventício. Nas estacas enraizadas, a origem do primórdio radicular foi próxima aos tecidos vasculares. Cortes histológicos nas raízes formadas nas estacas e nas raízes de mudas obtidas por semeadura confirmaram que elas apresentam as mesmas estruturas primárias.

  11. Allelopathic effects of two cover crops Commelina diffusa Burm. F. and Tradescantia zebrina Shunltz on Coffea arabica L.

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    Georgina Berroa Navarro

    2016-03-01

    Full Text Available Allelopathic effect of the cover crops Tradescantia zebrina Shunltz (cucaracha and Commelina diffusa Burm. F. (canutillo were evaluated on Coffea arabica Lin. seeds Caturra Rojo variety. Germination tests were carried out “in vitro” and it was evaluated the root longitude, percentage of total germination and period of germination, as well as the height of the plant and the emergency percentage for the incorporation tests to the soil. It was also carried out, to both over crops, the preliminary chemical qualitative characterization. The results showed that the extracts of T. zebrina and of C. diffusa stimulated the “in vitro” germination and growth of C. arabica at different concentration levels. The incorporation to the soil of the extracts of C. diffusa stimulated the development of the plants of C. arabica, in a significant way, that supposes a considerable advantage in that concerns to the employment of these cover crops, when not implying noxious effects beside all the benefits implied when using cover crops. These last ones go from the protection and improvement of the properties of the soil, to the control of the spontaneous flora in the coffee agroecosystems.

  12. EVALUACIÓN DE CUATRO ESPECIES FORESTALES ASOCIADAS CON CAFÉ (Coffea Arabica L. Y EN MONOCULTIVO EN EL LITORAL ECUATORIANO

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    Pedro Suatunce Cunuhay

    2009-12-01

    Full Text Available Se implantó un ensayo de sistema agroforestal con café (Coffea arabica L. en el área del cantón Quevedo, en la Finca Experimental “La Represa”, en el año de 1997. Se plantaron cuatro especies forestales en asociación con café (C. arabica L., y también se establecieron parcelas de árboles y café en monocultivo, en parcelas contiguas. Las especies forestales utilizadas fueron el guayacán blanco (Cybistax donnell-smithii Rose, laurel prieto (Cordia megalantha Chadat, fernansánchez (Triplaris cumingiana Wedd y teca (Tectona grandis L. F.. El objetivo fue comparar la producción de madera y café bajo los sistemas agroforestales y en los sistemas de plantación en monocultivo tanto de los árboles como del café. La producción por hectárea de café fue mayor en monocultivo. La incidencia (% del minador del café (Perileucoptera coffeella Green fue baja en los dos sistemas, además no hubo diferencias significativas entre los cafetales asociados y en monocultivo. El volumen de las especies forestales fue mayor en los sistemas agroforestales. Según la evaluación del uso eficiente de la tierra, cualquiera de los sistemas agroforestales evaluados es mejor que los cultivos puros de estas especies.

  13. Sensory Description of Cultivars (Coffea Arabica L. Resistant to Rust and Its Correlation with Caffeine, Trigonelline, and Chlorogenic Acid Compounds

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    Larissa de Oliveira Fassio

    2016-01-01

    Full Text Available Considering the importance of the chemical compounds in Arabica coffee beans in the definition of the drink sensory quality and authentication of coffee regions, the aim of this study was to evaluate, from principal component analysis—PCA—if there is a relation between the caffeine, trigonelline, and chlorogenic acid (5-CQA content and the sensory attributes of the drink, and in this context, enabling the differentiation of cultivars in two coffee-producing regions of Brazil. We evaluated seven rust-resistant Coffea arabica cultivars, and two rust-susceptible cultivars in two cultivation environments: Lavras, in the southern region of Minas Gerais state, and Patrocinio in the Cerrado region of Minas Gerais. The flavor and acidity were determinant for differentiation of the cultivars and their interaction with the evaluated environments. Cultivars Araponga MG1, Catigua MG2, and Catigua MG1 are the most suitable for the production of specialty coffee in the state of Minas Gerais. A poor correlation was found between caffeine, trigonelline, 5-CQA contents, and fragrance, flavor, acidity, body, and final score attributes. However, these compounds enabled the differentiation of the environments. The PCA indicated superiority in the sensory quality of cultivars resistant to rust, compared to the control, Bourbon Amarelo, and Topázio MG1190.

  14. Selection of Coffea canephora parents from the botanical varieties Conilon and Robusta for the production of intervarietal hybrids

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    Leilane Nicolino Lamarão de Oliveira

    2018-04-01

    Full Text Available ABSTRACT: The objective of this research was to quantify the genetic divergence of potential Coffea canephora parents, with the goal of developing progenies that associate the best traits of the Conilon and Robusta botanical varieties for hybrid vigor expression. Thus, 10 morphological and productive characteristics of 130 clones of Conilon and Robusta botanical varieties and their intervarietal hybrids were evaluated over 2 years. The experiment was conducted in a randomized block design with four replicates of four plants per plot. For selection of parents, the main component analysis was used to associate values with reference points obtained from the average of each botanical variety. The first two principal components allowed for the separation of the botanical varieties representing the variability contained in the original data with 76% for the first year and 69% for the second year. Although, the genotype × years interaction had significant effects, there were minor differences in the grouping from one year to the next, which is associated with the higher repeatability estimates observed in this study. It was observed that crosses with the 16-1-81I, 9-1-82L, and 13-1-61I parents of the botanical variety Robusta and the 167I, 890E, and 130I parents of the Conilon botanical variety presented greater potential for obtaining selection gains.

  15. Effect of CO2 on somatic embryos development Coffea arabica L. cv. ‘Caturra rojo’ and Clematis tangutica K.

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    Raúl Barbon

    2016-07-01

    Full Text Available Studies to optimize somatic embryogenesis have traditionally focused on the components of the culture medium but little other in vitro environment factors have been analyzed such as the composition of the gaseous atmosphere. The objective of this work was to determine the influence of CO2 on the development of the somatic embryo during the transition from the globular to the torpedo stage. The research was carried out on two model species for somatic embryogenesis that they are developed in different climatic zones: Coffea arabica L. cv. ‘Caturra rojo’ and Clematis tangutica K. Three CO2 concentrations (2.5, 5.0 and 10.0% combined with 21% O2 and two controls (passive exchange and forced ventilation were used. The effect of CO2 on the differentiation of somatic embryos from globular to torpedo stage in coffee and clematis was demonstrated, since in the treatments with passive exchange, where there was accumulation of CO2, the differentiation of somatic embryos was superior to treatments with forced ventilation. With 5.0% CO2 the process of differentiation of the embryos in the globular stage was stimulated, because in the treatment with this concentration of CO2 for coffee and clematis the highest proportion of embryos in torpedo stages and low levels of malformation were obtained.   Keywords: carbon dioxide, differentiation, in vitro environment, somatic embryogenesis

  16. Composition of the root mycorrhizal community associated with Coffea arabica in Fifa Mountains (Jazan region, Saudi Arabia).

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    Mahdhi, Mosbah; Tounekti, Taieb; Al-Turki, Turki Ali; Khemira, Habib

    2017-08-01

    Arbuscular mycorrhizal fungi (AMF) constitute a key functional group of soil biota that can greatly contribute to crop productivity and ecosystem sustainability. They improve nutrient uptake and enhance the ability of plants to cope with abiotic stresses. The presence of AMF in coffee (Coffea arabica L.) plant roots have been reported in several locations but not in Saudi Arabia despite the fact that coffee has been in cultivation here since ancient times. The objective of the present study was to investigate the diversity of AMF communities colonizing the roots of coffee trees growing in two sites of Fifa Mountains (south-west Saudi Arabia): site 1 at 700 m altitude and site 2 at 1400 m. The AMF large subunit rDNA regions (LSU) were subjected to nested PCR, cloning, sequencing, and phylogenetic analysis. Microscopic observations indicated higher mycorrhizal intensity (24.3%) and spore density (256 spores/100 g of soil) in site 2 (higher altitude). Phylogenetic analysis revealed 10 phylotypes, six belonging to the family Glomeraceae, two to Claroideoglomercea, one to Acaulosporaceae and one to Gigasporaceae family. Glomus was the dominant genus at both sites and the genus Gigaspora was detected only at site 2. This is the first study reporting the presence of AMF in coffee roots and the composition of this particular mycorrhizal community in Saudi Arabia. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Estudio preliminar de la comunidad de hongos endófitos en hojas de café (Coffea arabica

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    Mónica Milena Morales Valencia

    2006-01-01

    Full Text Available Los endófitos son organismos que viven asintomáticamente dentro de tejidos vegetales vivos. Este grupo no está restringido a los hongos sino que también se pueden encontrar bacterias e incluso invertebrados. Hasta el momento han sido poco estudiados, sobre todo en zonas tropicales. En el presente trabajo se hizo un estudio preliminar de la diversidad de la comunidad de hongos endófitos presentes en hojas de café (Coffea arabica en tres sitios del departamento de Cundinamarca, Colombia. Se muestrearon fragmentos foliares que representaron un área total de 2.700 mm2, procedentes de 15 árboles, en los cuales se encontraron 279 individuos de 38 morfotipos diferentes. El taxón más abundante fue Colletotrichum, el cual estuvo representado por el 47% de todos los individuos encontrados. Las comunidades de hongos endófitos estudiadas se ajustaron al modelo de distribución logarítmica normal, es decir, muchas especies raras y pocas abundantes.

  18. Aplicación de una técnica de Cromatografía de Exclusión molecular para la purificación de ADN en plantas de Coffea sp. APPLICATION OF A TECHNIQUE OF MOLECULAR EXCLUSION CHROMATOGRAPHY FOR THE PURIFICATION OF DNA FROM Coffea sp. PLANTS

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    Ana María García Cepero

    2006-12-01

    Full Text Available Uno de los mayores inconvenientes en la extracción y purificación de biomoléculas a partir de plantas del género Coffea, es un alto contenido de polifenoles y compuestos tánicos. En el presente artículo se describe una metodología que permite obtener ADN de alta pureza. La extracción del ADN del homogeneizado de tejido foliar en siete genotipos de Coffea sp., se realizó mediante la técnica citada por Chaparro (1993 y su purificación se logró mediante cromatografía de exclusión molecular sobre una fase estacionaria de Sephacryl S-1000. Los resultados muestran que la alta eficiencia de separación de ARN degradado, proteínas, pigmentos y compuestos que absorben entre 220 y 300 nm, permiten obtener un ADN de alta pureza a juzgar por los datos espectrofotométricos y electroforéticos.One of the greatest difficulties in extracting and purifying biomolecules from plants in the genus Coffea is the high polyphenol and tannin contents. In this study a methodology is described that allows obtaining high purity DNA from leaf tissues of seven genotypes of Coffea sp. by means of the technique desribed by Chaparro (1993 and its further purification was achieved by molecular exclusion chromatography on Sephacryl S-1000 (Pharmacia. The results showed that the high separation efficiency of degraded RNA, proteins, pigments, and other compounds that absorb between 220 and 300 nm allowed obtaining high purity DNA as judged by the spectophometric and electroforetic data.

  19. Single locus complementary sex determination in Hymenoptera: an "unintelligent" design?

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    Driessen Gerard

    2006-01-01

    Full Text Available Abstract The haplodiploid sex determining mechanism in Hymenoptera (males are haploid, females are diploid has played an important role in the evolution of this insect order. In Hymenoptera sex is usually determined by a single locus, heterozygotes are female and hemizygotes are male. Under inbreeding, homozygous diploid and sterile males occur which form a genetic burden for a population. We review life history and genetical traits that may overcome the disadvantages of single locus complementary sex determination (sl-CSD. Behavioural adaptations to avoid matings between relatives include active dispersal from natal patches and mating preferences for non-relatives. In non-social species, temporal and spatial segregation of male and female offspring reduces the burden of sl-CSD. In social species, diploid males are produced at the expense of workers and female reproductives. In some social species, diploid males and diploid male producing queens are killed by workers. Diploid male production may have played a role in the evolution or maintenance of polygyny (multiple queens and polyandry (multiple mating. Some forms of thelytoky (parthenogenetic female production increase homozygosity and are therefore incompatible with sl-CSD. We discuss a number of hypothetical adaptations to sl-CSD which should be considered in future studies of this insect order.

  20. A New Species of Vespula, and First Record of Vespa crabro L. (Hymenoptera:Vespidae) from Guatemala, Central America

    Science.gov (United States)

    Vespula akrei Landolt sp. nov. (Hymenoptera:Vespidae; Vespinae) is described from Guatemala. The first record of Vespa crabro L. (Hymenoptera:Vespidae:Vespinae) in Guatemala is given, and Vespula Inexspectata Eck (1994) from Mexico is re-described. We place Vespula akrei sp. nov. in the Vespula vulg...

  1. Caracterização e uso da variabilidade genética de banco ativo de germoplasma de Coffea canephora Pierre ex Froehner

    OpenAIRE

    Rocha, Rodrigo Barros; Embrapa Rondônia; Santos, Diogo Vieira; ULBRA; Ramalho, André Rostand; Embrapa Rondônia; Teixeira, Alexsandro Lara; Embrapa Rondônia

    2014-01-01

    Estratégias eficientes para manipular a variabilidade genética são determinantes para o sucesso dos programas de melhoramento de Coffea canephora. Combinações entre genitores divergentes expressam maior efeito heterótico, devendo a seleção de matrizes considerar, simultaneamente, a divergência genética e o desempenho agronômico superior.Objetivou-se, neste trabalho, quantificar a diversidade genética de acessos de C. canephora do Banco Ativo de Germoplasma da Embrapa Rondônia, visando à ident...

  2. Cinética da secagem de clones de café (Coffea canephora Pierre em terreiro de chão batido Kinetics of coffee berry clones drying (Coffea canephora Pierre in ground pavement

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    Osvaldo Resende

    2010-01-01

    Full Text Available Objetivou-se com o presente trabalho estudar a cinética da secagem de quatro clones de café da espécie Coffea canephora submetidos à secagem em terreiro de chão batido, bem como ajustar diferentes modelos matemáticos aos valores experimentais selecionando aquele que melhor representa o fenômeno em estudo. Foram utilizados frutos de café dos clones: Cpafro 194, Cpafro 193, Cpafro 167 e Cpafro180, colhidos com os teores de água iniciais de 1,20; 1,32; 1,51 e 1,46 (decimal base seca (b.s., respectivamente. A secagem prosseguiu em terreiro de chão batido até que o produto atingisse o teor de água de 0,137; 0,133; 0,142 e 0,140 (decimal b.s. respectivamente para os clones Cpafro 194, Cpafro 193, Cpafro 167 e Cpafro 180. Aos dados experimentais foram ajustados dez modelos matemáticos citados na literatura específica e utilizados para representação do processo de secagem de produtos agrícolas. Baseando-se em parâmetros estatísticos, conclui-se que os modelos Verma, Dois Termos e Aproximação da Difusão foram adequados para representação da secagem dos quatro clones de café analisados, e além destes, para o clone Cpafro 167, os modelos Thompson, Page, Newton, Logarítmico, Henderson e Pabis e Exponencial de Dois Termos também se mostraram satisfatórios na descrição do fenômeno; já o tempo necessário para a secagem em terreiro de chão batido dos clones de café Cpafro 194, Cpafro 193, Cpafro 167 e Cpafro 180 foi de 189,5 h.The objective of this work was to study the drying kinetics of four coffee berry clones Coffea canephora species dried in ground pavement and to fit different mathematical models to different experimental data selecting the one that best represents the phenomenon. Have been used coffee berry of clones: Cpafro 194, Cpafro 193, Cpafro 167 and Cpafro 180, harvested at moisture content of 1.20; 1.32; 1.51 e 1.46 (decimal d.b., respectively. The drying continued in ground pavement until achieved the moisture

  3. Avaliação da fertilidade dos solos de sistemas agroflorestais com cafeeiro (Coffea arabica L. em Lavras-MG Evaluation of soil fertility in agroforest systems with coffee trees (Coffea arabica L. in Lavras-MG

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    Bruno Grandi Salgado

    2006-06-01

    Full Text Available Com o objetivo de avaliar a fertilidade dos solos em um sistema agroflorestal composto por cafeeiros (Coffea arábica L. - Mundo Novo, ingazeiros (Inga vera Willd e grevíleas (Grevilea robusta A. Cunn, situado em Lavras, Minas Gerais, foi instalado o presente experimento. Usou-se um delineamento experimental inteiramente casualizado com três tratamentos e sete repetições. Os tratamentos foram: a cafeeiros a pleno sol, b cafeeiros consorciados com ingazeiros e c cafeeiros consociados com grevílea. Os espaçamentos dos cafeeiros nos três sistemas foi 4 x 1 m, para o ingazeiro 8 m x 15 m e para a grevílea 12 m x 10 m. Aos 15 anos de idade do cafeeiro e do ingazeiro e aos 9 anos da grevílea foram avaliadas as seguintes características dos solos, pH, acidez potencial (H+Al, alumínio trocável (Al+3, bases trocáveis (Ca+2 e Mg+2, potássio disponível (K+, fósforo disponível(P, enxofre (S, CTC efetiva (T, soma de bases (SB, saturação de bases (V e matéria orgânica (MO. Os resultados foram submetidos à analise de variância e as médias, comparadas pelo teste de Scott-Knott. Após a análise dos dados, concluiu-se que, embora tenha havido diferenças entre alguns elementos estudados, as características químicas dos solos nos três sistemas não foram severamente afetadas.The objective of this wark was to evaluat e the soil fertility in an agroforest system using coffee trees (Coffea arabica L - Mundo Novo, inga trees (Inga vera Wild and grevillea trees (Grevillea robusta A Cunn situated in Lavras, Minas Geris. A completely randomized experimental design with tree treatments and seven replicates was utilized. The treatments were : a coffee trees in full sunshine; b coffee trees mixed with inga trees and c coffee trees mixed with grevillea trees. Tree spacings in the three systems were 4 x 1m for coffee, 8 m x 15 m for inga and 12 x 10 m for grevillea. With coffee and inga trees at 15 years of age and grevillea trees at 9 years of age

  4. Genética de Coffea. XVIII - Variegação no cafeeiro Leaf variegation in coffee plants

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    A. Carvalho

    1954-01-01

    Full Text Available A variegação das fôlhas do cafeeiro tanto em plantas novas como em plantas adultas tem sido freqüentemente observada. Plantas variegadas podem ocorrer espontaneamente nas progénies das diversas variedades de Coffea arabica L., motivo porque não devem constituir variedade distinta dessa espécie, como foi proposto por Cramer. As plantas variegadas em estudo puderam ser agrupadas em duas categorias, isto é, aquelas em que a variegação não se acha associada com anomalias na forma e textura das folhas e aquelas nas quais a variegação é acompanhada dessas anomalias. No primeiro grupo o padrão de variegação não é uniforme para tôdas as fôlhas, enquanto no segundo grupo o padrão é semelhante para tôda a planta. Usualmente apenas alguns ramos das plantas do primeiro grupo são variegados. Observou-se algumas vezes que as duas fôlhas do mesmo verticilo têm padrões semelhantes de variegação. Encontraram-se também fôlhas variegadas nas quais a metade da lâmina é variegada ou mesmo albina, enquanto a outra metade apresenta a côr verde normal. Os dados genéticos obtidos, embora preliminares para a maioria das plantas variegadas em investigação, permitiram concluir que o padrão de variegação da planta 180, pertencente ao primeiro grupo, é herdado pelo citoplasma e não é transmitido pelo pólen. O padrão de variegação da planta 253-21, pertencente ao segundo grupo, no entanto, parece ser transmitido pelo polen.Leaf variegation in coffee plants is a relatively common abnormality, and variegated seedlings have been found to occur spontaneously in progenies of most varieties of Coffea arabica L. For this reason it is thought that variegated types should not be described as distinct varieties, as it was done before by Cramer. Variegated coffee plants can be grouped in two categories : first, those in which variegated leaves are normal in shape and texture, but do not show a uniform variegation pattern; and second, those

  5. Agrobacterium-mediated genetic transformation of Coffea arabica (L. is greatly enhanced by using established embryogenic callus cultures

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    Lashermes Philippe

    2011-05-01

    Full Text Available Abstract Background Following genome sequencing of crop plants, one of the main challenges today is determining the function of all the predicted genes. When gene validation approaches are used for woody species, the main obstacle is the low recovery rate of transgenic plants from elite or commercial cultivars. Embryogenic calli have frequently been the target tissue for transformation, but the difficulty in producing or maintaining embryogenic tissues is one of the main problems encountered in genetic transformation of many woody plants, including Coffea arabica. Results We identified the conditions required for successful long-term proliferation of embryogenic cultures in C. arabica and designed a highly efficient and reliable Agrobacterium tumefaciens-mediated transformation method based on these conditions. The transformation protocol with LBA1119 harboring pBin 35S GFP was established by evaluating the effect of different parameters on transformation efficiency by GFP detection. Using embryogenic callus cultures, co-cultivation with LBA1119 OD600 = 0.6 for five days at 20 °C enabled reproducible transformation. The maintenance conditions for the embryogenic callus cultures, particularly a high auxin to cytokinin ratio, the age of the culture (optimum for 7-10 months of proliferation and the use of a yellow callus phenotype, were the most important factors for achieving highly efficient transformation (> 90%. At the histological level, successful transformation was related to the number of proembryogenic masses present. All the selected plants were proved to be transformed by PCR and Southern blot hybridization. Conclusion Most progress in increasing transformation efficiency in coffee has been achieved by optimizing the production conditions of embryogenic cultures used as target tissues for transformation. This is the first time that a strong positive effect of the age of the culture on transformation efficiency was demonstrated. Our

  6. Impacts of leaf age and heat stress duration on photosynthetic gas exchange and foliar nonstructural carbohydrates in Coffea arabica.

    Science.gov (United States)

    Marias, Danielle E; Meinzer, Frederick C; Still, Christopher

    2017-02-01

    Given future climate predictions of increased temperature, and frequency and intensity of heat waves in the tropics, suitable habitat to grow ecologically, economically, and socially valuable Coffea arabica is severely threatened. We investigated how leaf age and heat stress duration impact recovery from heat stress in C. arabica . Treated plants were heated in a growth chamber at 49°C for 45 or 90 min. Physiological recovery was monitored in situ using gas exchange, chlorophyll fluorescence (the ratio of variable to maximum fluorescence, F V / F M ), and leaf nonstructural carbohydrate (NSC) on mature and expanding leaves before and 2, 15, 25, and 50 days after treatment. Regardless of leaf age, the 90-min treatment resulted in greater F V / F M reduction 2 days after treatment and slower recovery than the 45-min treatment. In both treatments, photosynthesis of expanding leaves recovered more slowly than in mature leaves. Stomatal conductance ( g s ) decreased in expanding leaves but did not change in mature leaves. These responses led to reduced intrinsic water-use efficiency with increasing heat stress duration in both age classes. Based on a leaf energy balance model, aftereffects of heat stress would be exacerbated by increases in leaf temperature at low g s under full sunlight where C. arabica is often grown, but also under partial sunlight. Starch and total NSC content of the 45-min group significantly decreased 2 days after treatment and then accumulated 15 and 25 days after treatment coinciding with recovery of photosynthesis and F V / F M . In contrast, sucrose of the 90-min group accumulated at day 2 suggesting that phloem transport was inhibited. Both treatment group responses contrasted with control plant total NSC and starch, which declined with time associated with subsequent flower and fruit production. No treated plants produced flowers or fruits, suggesting that short duration heat stress can lead to crop failure.

  7. Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures

    Science.gov (United States)

    2011-01-01

    Background Following genome sequencing of crop plants, one of the main challenges today is determining the function of all the predicted genes. When gene validation approaches are used for woody species, the main obstacle is the low recovery rate of transgenic plants from elite or commercial cultivars. Embryogenic calli have frequently been the target tissue for transformation, but the difficulty in producing or maintaining embryogenic tissues is one of the main problems encountered in genetic transformation of many woody plants, including Coffea arabica. Results We identified the conditions required for successful long-term proliferation of embryogenic cultures in C. arabica and designed a highly efficient and reliable Agrobacterium tumefaciens-mediated transformation method based on these conditions. The transformation protocol with LBA1119 harboring pBin 35S GFP was established by evaluating the effect of different parameters on transformation efficiency by GFP detection. Using embryogenic callus cultures, co-cultivation with LBA1119 OD600 = 0.6 for five days at 20 °C enabled reproducible transformation. The maintenance conditions for the embryogenic callus cultures, particularly a high auxin to cytokinin ratio, the age of the culture (optimum for 7-10 months of proliferation) and the use of a yellow callus phenotype, were the most important factors for achieving highly efficient transformation (> 90%). At the histological level, successful transformation was related to the number of proembryogenic masses present. All the selected plants were proved to be transformed by PCR and Southern blot hybridization. Conclusion Most progress in increasing transformation efficiency in coffee has been achieved by optimizing the production conditions of embryogenic cultures used as target tissues for transformation. This is the first time that a strong positive effect of the age of the culture on transformation efficiency was demonstrated. Our results make Agrobacterium

  8. Diversity and communities of foliar endophytic fungi from different agroecosystems of Coffea arabica L. in two regions of Veracruz, Mexico.

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    Saucedo-García, Aurora; Anaya, Ana Luisa; Espinosa-García, Francisco J; González, María C

    2014-01-01

    Over the past 20 years, the biodiversity associated with shaded coffee plantations and the role of diverse agroforestry types in biodiversity conservation and environmental services have been topics of debate. Endophytic fungi, which are microorganisms that inhabit plant tissues in an asymptomatic manner, form a part of the biodiversity associated with coffee plants. Studies on the endophytic fungi communities of cultivable host plants have shown variability among farming regions; however, the variability in fungal endophytic communities of coffee plants among different coffee agroforestry systems is still poorly understood. As such, we analyzed the diversity and communities of foliar endophytic fungi inhabiting Coffea arabica plants growing in the rustic plantations and simple polycultures of two regions in the center of Veracruz, Mexico. The endophytic fungi isolates were identified by their morphological traits, and the majority of identified species correspond to species of fungi previously reported as endophytes of coffee leaves. We analyzed and compared the colonization rates, diversity, and communities of endophytes found in the different agroforestry systems and in the different regions. Although the endophytic diversity was not fully recovered, we found differences in the abundance and diversity of endophytes among the coffee regions and differences in richness between the two different agroforestry systems of each region. No consistent pattern of community similarity was found between the coffee agroforestry systems, but we found that rustic plantations shared the highest number of morphospecies. The results suggest that endophyte abundance, richness, diversity, and communities may be influenced predominantly by coffee region, and to a lesser extent, by the agroforestry system. Our results contribute to the knowledge of the relationships between agroforestry systems and biodiversity conservation and provide information regarding some endophytic fungi and

  9. Identification by the DArTseq method of the genetic origin of the Coffea canephora cultivated in Vietnam and Mexico.

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    Garavito, Andrea; Montagnon, Christophe; Guyot, Romain; Bertrand, Benoît

    2016-11-04

    The coffee species Coffea canephora is commercially identified as "Conilon" when produced in Brazil, or "Robusta" when produced elsewhere in the world. It represents approximately 40 % of coffee production worldwide. While the genetic diversity of wild C. canephora has been well studied in the past, only few studies have addressed the genetic diversity of currently cultivated varieties around the globe. Vietnam is the largest Robusta producer in the world, while Mexico is the only Latin American country, besides Brazil, that has a significant Robusta production. Knowledge of the genetic origin of Robusta cultivated varieties in countries as important as Vietnam and Mexico is therefore of high interest. Through the use of Sequencing-based diversity array technology-DArTseq method-on a collection of C. canephora composed of known accessions and accessions cultivated in Vietnam and Mexico, 4,021 polymorphic SNPs were identified. We used a multivariate analysis using SNP data from reference accessions in order to confirm and further fine-tune the genetic diversity of C. canephora. Also, by interpolating the data obtained for the varieties from Vietnam and Mexico, we determined that they are closely related to each other, and identified that their genetic origin is the Robusta Congo - Uganda group. The genetic characterization based on SNP markers of the varieties grown throughout the world, increased our knowledge on the genetic diversity of C. canephora, and contributed to the understanding of the genetic background of varieties from very important coffee producers. Given the common genetic origin of the Robusta varieties cultivated in Vietnam, Mexico and Uganda, and the similar characteristics of climatic areas and relatively high altitude where they are grown, we can state that the Vietnamese and the Mexican Robusta have the same genetic potential to produce good cup quality.

  10. Proteomic analysis of apoplastic fluid of Coffea arabica leaves highlights novel biomarkers for resistance against Hemileia vastatrix

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    Leonor eGuerra-Guimarães

    2015-06-01

    Full Text Available A proteomic analysis of the apoplastic fluid (APF of coffee leaves was conducted to investigate the cellular processes associated with incompatible (resistant and compatible (susceptible Coffea arabica-Hemileia vastatrix interactions, during the 24-96 hai period. The APF proteins were extracted by leaf vacuum infiltration and protein profiles were obtained by 2-DE. The comparative analysis of the gels revealed 210 polypeptide spots whose volume changed in abundance between samples (control, resistant and susceptible during the 24-96 hai period. The proteins identified were involved mainly in protein degradation, cell wall metabolism and stress/defense responses, most of them being hydrolases (around 70%, particularly sugar hydrolases and peptidases/proteases. The changes in the APF proteome along the infection process revealed two distinct phases of defense responses, an initial/basal one (24-48 hai and a late/specific one (72-96 hai. Compared to susceptibility, resistance was associated with a higher number of proteins, which was more evident in the late/specific phase. Proteins involved in the resistance response were mainly, glycohydrolases of the cell wall, serine proteases and pathogen related-like proteins (PR-proteins, suggesting that some of these proteins could be putative candidates for resistant markers of coffee to H. vastatrix. Antibodies were produced against chitinase, pectin methylesterase, serine carboxypeptidase, reticuline oxidase and subtilase and by an immunodetection assay it was observed an increase of these proteins in the resistant sample. With this methodology we have identified proteins that are candidate markers of resistance and that will be useful in coffee breeding programs to assist in the selection of cultivars with resistance to H. vastatrix.

  11. Respuesta fotosintética de Coffea arabica L. a diferentes niveles de luz y disponibilidad hídrica

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    Yesid Alejandro Mariño

    2014-04-01

    Full Text Available El sombrío permite una mejor aclimatación del café (Coffea arabica L. en zonas donde el invierno se caracteriza por bajas temperaturas nocturnas seguido de días soleados y el verano, por altas temperaturas y lluvias ocasionales. En este estudio, realizado en Viçosa-MG, Brasil, se analizaron los efectos de dos condiciones de luminosidad (pleno sol y 15% de la radiación solar y disponibilidades de agua en el suelo sobre las relaciones hídricas y el desempeño fotosintético en C. arabica en dos épocas contrastantes. Independientemente de la época de evaluación, las plantas cultivadas en condiciones de baja disponibilidad hídrica presentaron reducciones en la fotosíntesis (A, conductancia estomática (g s y en la razón Ci/Ca en los dos tratamientos lumínicos. Estos cambios fueron acompañados con la reducción en la conductancia hidráulica y la transpiración. No se presentaron diferencias significativas en la producción cuántica efectiva, en el coeficiente de extinción no-fotoquímica y en la productividad cuántica basal de los procesos no fotoquímicos. Sobre la disponibilidad hídrica adecuada, las plantas cultivadas al sol presentaron reducciones en A, g s y Ci/Ca en la época de invierno. Adicionalmente, se encontraron limitaciones fotoquímicas con la reducción en la razón de las fluorescencias variable e inicial. Los resultados indican que cualquier recomendación de uso de sombrío como práctica de manejo debe ser tomada con precaución.

  12. Mortalidade de Coccus viridis (Hemiptera: Coccidae por Lecanicillium spp. em diferentes órgãos de Coffea arabica

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    Flávio Lemes Fernandes

    2009-04-01

    Full Text Available Objetivou-se neste trabalho avaliar a mortalidade de Coccus viridis por Lecanicillium spp. em folhas e ramos de Coffea arabica. O trabalho foi realizado na casa de vegetação na Universidade Federal de Viçosa, entre fevereiro de 2005 a junho de 2006. Foram utilizadas sementes da variedade Catuaí vermelho de café (C. arabica. A solução nutritiva utilizada foi composta por 3,0 mmol/L de N, 9,0 mmol/L de K, 1,0 mmol/L de P, 4,0 mmol/L de Ca, 2,1 mmol/L de S, 46 µmol/L de B, 0,3 µmol/L de Cu, 60,0 µmol/L de Fe, 2,0 µmol/L de Mg, 0,5 µmol/L de Mo e 36,0 µmol/L de Mn. As cochonilhas e o fungo foram coletados de folhas de café em lavouras comerciais de Viçosa, MG e foram mantidos em gaiolas em Casa de Vegetação. As plantas foram infestadas com oito meses de idade. A infestação foi realizada por contato de C. viridis infectadas com o fungo sobre as cochonilhas sadias. As densidades de C. viridis viva e morta nas folhas e nos ramos foram avaliadas aos 0, 15, 30, 35, 45, 60 e 75 dias após a infestação. Usou-se análise de variância, teste de média de Skott-Knott e análise de regressão linear simples a p<0,05. O fungo Lecanicillium spp. causou mortalidade em todos os estádios de C. viridis. De forma geral, a maior mortalidade ocorreu no terceiro ínstar. Além disso, o fungo causou maior mortalidade nesse inseto praga na face abaxial da folha de café. Mortality of Coccus viridis (Hemiptera: Coccidae by Lecanicillium spp. in differents Coffea arabica organs in greenhouseAbstract. The objective of this work was to evaluate the mortality of Coccus viridis by Lecanicillium spp. on faces of the leaves, and branches of Coffea arabica. This work was conducted in greenhouse at UFV. The experiment was carried out from February 2005 to June 2006. Seeds of the lineage IAC 15 from the variety “Catuaí Vermelho” coffee (C. arabica were used. The nutritive solution utilized was composed for 3. 0 mmol/L of N, 9. 0 mmol/L of K, 1. 0 mmol

  13. An endoplasmic reticulum-localized Coffea arabica BURP domain-containing protein affects the response of transgenic Arabidopsis plants to diverse abiotic stresses.

    Science.gov (United States)

    Dinh, Sy Nguyen; Kang, Hunseung

    2017-11-01

    The Coffea arabica BURP domain-containing gene plays an important role in the response of transgenic Arabidopsis plants to abiotic stresses via regulating the level of diverse proteins. Although the functions of plant-specific BURP domain-containing proteins (BDP) have been determined for a few plants, their roles in the growth, development, and stress responses of most plant species, including coffee plant (Coffea arabica), are largely unknown. In this study, the function of a C. arabica BDP, designated CaBDP1, was investigated in transgenic Arabidopsis plants. The expression of CaBDP1 was highly modulated in coffee plants subjected to drought, cold, salt, or ABA. Confocal analysis of CaBDP1-GFP fusion proteins revealed that CaBDP1 is localized in the endoplasmic reticulum. The ectopic expression of CaBDP1 in Arabidopsis resulted in delayed germination of the transgenic plants under abiotic stress and in the presence of ABA. Cotyledon greening and seedling growth of the transgenic plants were inhibited in the presence of ABA due to the upregulation of ABA signaling-related genes like ABI3, ABI4, and ABI5. Proteome analysis revealed that the levels of several proteins are modulated in CaBDP1-expressing transgenic plants. The results of this study underscore the importance of BURP domain proteins in plant responses to diverse abiotic stresses.

  14. KEANEKARAGAMAN HYMENOPTERA PARASITOID PADA PERKEBUNAN KELAPA SAWIT PTPN VIII CINDALI, BOGOR

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    Ichsan Luqmana Indra Putra

    2016-09-01

    Full Text Available Diversity of parasitic Hymenoptera in PTPN VIII oil palm plantation Cindali, Bogor. One group of important natural enemies in oil palm plantation is parasitic Hymenoptera. The purpose of this research is to know the diversity and fluctuation of parasitic Hymenoptera PTPN VIII Cindali oil palm plantation. This research was conducted in 6 blocks of oil palm in September 2014 – June 2015. There were 5 plots in every observation blocks 39.2 x 39.2 m in size, and used direct and indirect method. Direct method done by 5 plants in every plots was taken randomized to observed and taken the herbivore insects to reared until the parasitic Hymenoptera came out. Observation of cover crops conducted by 3 subplots determined diagonally in every plots 9.8 x 9.8 m in size and herbivore insects was observed and collected. Indirect methods used sweep net and yellow pan trap. The result of this research, 26 parasitic Hymenoptera families was found, with the Braconidae was the most morphospecies found and the most individual amount was Scelionidae. The abundance of parasitoid in every month fluctuated.

  15. The Community of Hymenoptera Parasitizing Necrophagous Diptera in an Urban Biotope

    Science.gov (United States)

    Frederickx, Christine; Dekeirsschieter, Jessica; Verheggen, François J.; Haubruge, Eric

    2013-01-01

    Most reports published in the field of forensic entomology are focused on Diptera and neglect the Hymenoptera community. However, Hymenoptera are part of the entomofaunal colonization of a dead body. The use of Hymenoptera parasitoids in forensic entomology can be relevant to evaluate the time of death. Hymenoptera parasitoids of the larvae and pupae of flies may play an important role in the estimation of the post-mortem period because their time of attack is often restricted to a small, well-defined window of time in the development of the host insect. However, these parasitoids can interfere with the developmental times of colonizing Diptera, and therefore a better understanding of their ecology is needed. The work reported here monitored the presence of adult Hymenoptera parasitoids on decaying pig carcasses in an urban biotope during the summer season (from May to September). Six families and six species of parasitoids were recorded in the field: Aspilota fuscicornis Haliday (Braconidae), Alysia manducator Panzer, Nasonia vitripennis Walker (Pteromalidae), Tachinaephagus zealandicus Ashmead (Encyrtidae), Trichopria sp. (Diapriidae), and Figites sp. (Figitidae). In the laboratory, five species emerged from pupae collected in the field: Trichopria sp., Figites sp., A. manducator, N. vitripennis, and T. zealandicus. These five species colonize a broad spectrum of Diptera hosts, including those species associated with decomposing carcasses, namely those from the families Calliphoridae, Muscidae, Fanniidae, and Sarcophagidae. PMID:23895458

  16. On the identity of Melipona torrida Friese (Hymenoptera, Apidae

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    Gabriel A. R. Melo

    2013-09-01

    Full Text Available On the identity of Melipona torrida Friese (Hymenoptera, Apidae. Melipona marginata var. torrida Friese, 1916, described from three workers putatively collected in Costa Rica, never had its identity properly recognized. Since its original description, no additional specimens have ever been collected in Costa Rica. It is argued here that Melipona torrida was based on mislabeled specimens and corresponds to Melipona marginata obscurior Moure, 1971, a form known only from southern Brazil, Argentina and Paraguay. A lectotype is designated for Melipona torrida and notes on the type material of Melipona marginata obscurior are provided. Other known examples of species described from mislabeled specimens in Friese's Zur Bienenfauna von Costa Rica are discussed. It is pointed out that additional names proposed in this work, based on material from Costa Rica, might turn out to correspond to South American taxa. Also, the date of publication of this Friese's paper is discussed.

  17. Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae by fluorochromes

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    Jefferson de Brito Marthe

    2010-01-01

    Full Text Available Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae were cytogenetically analyzed using conventional staining and the fluorochromes CMA3 e DAPI. The females have 2n = 34 chromosomes (2K=32+2. Some females, however, presented an additional large B acrocentric chromosome, to a total of 2n = 35. Chromosome B and the chromosomal pairs 2, 9 and 10 showed CMA3+ bands, indicating an excess of CG base-pairs. A clear association was verified between the P. helleri B chromosome SCAR marker and the presence of a B chromosome in P. cupira. The data obtained suggests that B chromosomes in P. helleri and P. cupira share a common origin.

  18. ABO blood groups, Rhesus factor, and anaphylactic reactions due to Hymenoptera stings.

    Science.gov (United States)

    Pałgan, Krzysztof; Bartuzi, Zbigniew; Chrzaniecka, Elżbieta

    2017-09-21

    Numerous publications indicate that the prevalence of some infectious, neoplastic and immunological diseases are associated with ABO blood groups. The aim of this study was to verify whether ABO and Rh blood groups are associated with severe anaphylactic reactions after Hymenoptera stings. A study was undertaken of 71,441 Caucasian subjects living in the same geographic area. The study group included 353 patients with diagnosed systemic anaphylaxis to Hymenoptera venom. Control group included 71,088 healthy blood donors. Frequencies of ABO and Rhesus groups in the study and control groups were compared using univariate and multivariate analyses. No statistically significant interactions were observed between the ABO blood group and anaphylactic reactions to Hymenoptera.

  19. Apomictic parthenogenesis in a parasitoid wasp Meteorus pulchricornis, uncommon in the haplodiploid order Hymenoptera.

    Science.gov (United States)

    Tsutsui, Y; Maeto, K; Hamaguchi, K; Isaki, Y; Takami, Y; Naito, T; Miura, K

    2014-06-01

    Although apomixis is the most common form of parthenogenesis in diplodiploid arthropods, it is uncommon in the haplodiploid insect order Hymenoptera. We found a new type of spontaneous apomixis in the Hymenoptera, completely lacking meiosis and the expulsion of polar bodies in egg maturation division, on the thelytokous strain of a parasitoid wasp Meteorus pulchricornis (Wesmael) (Braconidae, Euphorinae) on pest lepidopteran larvae Spodoptera litura (Fabricius) (Noctuidae). The absence of the meiotic process was consistent with a non-segregation pattern in the offspring of heterozygous females, and no positive evidence was obtained for the induction of thelytoky by any bacterial symbionts. We discuss the conditions that enable the occurrence of such rare cases of apomictic thelytoky in the Hymenoptera, suggesting the significance of fixed heterosis caused by hybridization or polyploidization, symbiosis with bacterial agents, and occasional sex. Our finding will encourage further genetic studies on parasitoid wasps to use asexual lines more wisely for biological control.

  20. A new scenario of bioprospecting of Hymenoptera venoms through proteomic approach

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    LD Santos

    2011-01-01

    Full Text Available Venoms represent a huge and essentially unexplored reservoir of bioactive components that may cure diseases that do not respond to currently available therapies. This review select advances reported in the literature from 2000 to the present about the new scenario of Hymenoptera venom composition. On account of new technologies in the proteomic approach, which presents high resolution and sensitivity, the combination of developments in new instruments, fragmentation methods, strategic analysis, and mass spectrometry have become indispensable tools for interrogation of protein expression, molecule interaction, and post- translational modifications. Thus, the biochemical characterization of Hymenoptera venom has become a major subject of research in the area of allergy and immunology, in which proteomics has been an excellent alternative to assist the development of more specific extracts for diagnosis and treatment of hypersensitive patients to Hymenoptera venoms.

  1. Morphometric and Productive Characterization of Nineteen Genotypes from the Colombian Coffea Collection / Caracterización Morfométrica y Productiva de Diecinueve Genotipos de la Colección Colombiana del Género Coffea

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    Jhon Wilson Mejía Montoya

    2013-12-01

    Full Text Available Nineteen genotypes of the Colombian Coffee (Coffeaarabica L. Collection were characterized through features related to productivity, crown architecture and light interception. The results revealed significant differences among genotypes. Branches and leaves were found to be dominantly plagiophyl. Leaf area (LA and Leaf area index (LAI made accession CU1812 (which correspondsto variety Castillo® stand out for its photosynthetically activeradiation (PAR interception and coffee bean production. Likewise, a PAR based cluster analysis allowed dividing the genotypes in three groups. Because of their higher yield, the most outstanding genotypes were Caturra, CU-1812 and Harrar R2. This factor showed correlation with PAR absorption. The current results are useful for future works in coffee breeding programs. / Se caracterizaron 19 genotipos de la Colección Colombiana de Café (Coffea arabica L.; para ello se estudiaron aspectos relacionados con la arquitectura del dosel, la interceptación de la radiación y la producción. Los resultados mostraron diferencias significativas entre los genotipos, predominando en las ramas la distribución plagiofila; una disposición similar se observóen las hojas. El área foliar (AF y el índice de área foliar (IAF,contribuyeron a que la introducción CU1812, componente de la variedad Castillo®, se destacara por presentar los mayores valores de interceptación de radiación fotosintéticamente activa (RFA y producción de café cereza. Así mismo, el análisis de agrupamiento con base en la RFA, permitió ordenar los genotipos en tres grupos,destacándose Caturra, CU-1812 y Harrar R2 por su mayorrendimiento, factor que mostró correlación con la RFA captadapor el dosel. Los resultados obtenidos en esta investigación son de utilidad en futuros trabajos de mejoramiento genético en café.

  2. Espécies novas de Trigonisca Moure (Hymenoptera, Apidae, Apinae New species of Trigonisca Moure (Hymenoptera, Apidae, Apinae

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    Patrícia Maia Correia de Albuquerque

    2007-01-01

    Full Text Available Algumas espécies novas de Meliponini do gênero Trigonisca Moure, 1950 (Hymenoptera, Apidae, Apinae do Brasil e Panamá são descritas: T. roubiki sp. nov. (Panamá, T. variegatifrons sp. nov. (Brasil: RO, PA, MT, T. vitrifrons sp. nov. (Brasil: AM, PA, T. unidentata sp. nov. (Brasil: AM, T. meridionalis sp. nov. (Brasil: PA, MA, MT, MG, SP, T. bidentata sp. nov. (Brasil: RO, T. extrema sp. nov. (Brasil: AM e T. hirticornis sp. nov. (Brasil: RO, PA; e são apresentados novos registros geográficos de T. flavicans (Moure, 1950, T. intermedia Moure, 1989, T. dobzhanskyi (Moure, 1950, T. ceophloei (Schwarz, 1938, T. nataliae (Moure, 1950 e T. pediculana (Fabricius, 1804 e uma chave para identificação das espécies.Some new Meliponini species of the genus Trigonisca Moure, 1950 (Hymenoptera, Apidae, Apinae from Brazil and Panamá are described: T. roubiki sp. nov. (Panamá, T. variegatifrons sp. nov. (Brazil: RO, PA, MT, T. vitrifrons sp. nov. (Brazil: AM, PA, T. unidentata sp. nov. (Brazil: AM, T. meridionalis sp. nov. (Brazil: PA, MA, MT, MG, SP, T. bidentata sp. nov. (Brazil: RO, T. extrema sp. nov. (Brazil: AM and T. hirticornis sp. nov. (Brazil: RO, PA; additional geographic records from T. flavicans (Moure, 1950, T. intermedia Moure, 1989, T. dobzhanskyi (Moure, 1950, T. ceophloei (Schwarz, 1938, T. nataliae (Moure, 1950 and T. pediculana (Fabricius, 1804 and an identification key for species are presented.

  3. Epidemiology, diagnosis, and treatment of Hymenoptera venom allergy in mastocytosis patients.

    Science.gov (United States)

    Niedoszytko, Marek; Bonadonna, Patrizia; Oude Elberink, Joanne N G; Golden, David B K

    2014-05-01

    Hymenoptera venom allergy is a typical IgE-mediated reaction caused by sensitization to 1 or more allergens of the venom, and accounts for 1.5% to 34% of all cases of anaphylaxis. Patients suffering from mastocytosis are more susceptible to the anaphylactic reactions to an insect sting. This article aims to answer the most important clinical questions raised by the diagnosis and treatment of insect venom allergy in mastocytosis patients. Total avoidance of Hymenoptera is not feasible, and there is no preventive pharmacologic treatment available, although venom immunotherapy reduces the risk of subsequent systemic reactions. Copyright © 2014 Elsevier Inc. All rights reserved.

  4. Caracterización de genotipos élite de Coffea canephora por su tolerancia a sequía.

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    Ana Odetth Quintana-Escobar

    2016-12-01

    Full Text Available El objetivo del presente trabajo fue identi car el grado de tolerancia a sequía en diferentes genotipos élite de Coffea canephora P. var. Robusta. El estudio se realizó del 2013 al 2015 en Tuxtla Chico, Chiapas, México. De cada genotipo (INIFAP P9, INIFAP P4, INIFAP P5, INIFAP P7, INIFAP P8 se utilizaron veinte plantas, excepto para el INIFAP P9 con doce; un grupo con la mitad de plantas de cada genotipo se mantuvo en condiciones de capacidad de campo (CC y la otra mitad en punto de marchitez permanente (PMP. Al nalizar la etapa de estrés y después del riego de recuperación, se evaluaron variables morfológicas y siológicas, tales como parámetros de crecimiento, contenido relativo de agua, acumulación de prolina, índice y densidad estomática e índice de tolerancia a sequía. Los genotipos de C. canephora presentaron entre ellos diferente grado de tolerancia al dé cit hídrico, pero compartieron entre sí la detención de sus funciones y activación de diferentes rutas para tolerar la sequía, como disminución en el contenido relativo de agua y aumento de prolina durante el periodo de estrés. Al determinar el índice de tolerancia a sequía, el genotipo más tolerante fue el INIFAP P5 de acuerdo con las variables número de hojas retenidas y totales, tasa de crecimiento relativo, número de pares de ramas plagiotrópicas y nudos totales; mientras que el INIFAP P4 se identi có como uno de los más susceptibles de acuerdo con la tasa de crecimiento relativo y el número de nudos totales, en las cuales presentó los valores más bajos.

  5. Evolutionary Expansion of WRKY Gene Family in Banana and Its Expression Profile during the Infection of Root Lesion Nematode, Pratylenchus coffeae

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    Suthanthiram, Backiyarani; Subbaraya, Uma; Marimuthu Somasundram, Saraswathi; Muthu, Mayilvaganan

    2016-01-01

    The WRKY family of transcription factors orchestrate the reprogrammed expression of the complex network of defense genes at various biotic and abiotic stresses. Within the last 96 million years, three rounds of Musa polyploidization events had occurred from selective pressure causing duplication of MusaWRKYs with new activities. Here, we identified a total of 153 WRKY transcription factors available from the DH Pahang genome. Based on their phylogenetic relationship, the MusaWRKYs available with complete gene sequence were classified into the seven common WRKY sub-groups. Synteny analyses data revealed paralogous relationships, with 17 MusaWRKY gene pairs originating from the duplication events that had occurred within the Musa lineage. We also found 15 other MusaWRKY gene pairs originating from much older duplication events that had occurred along Arecales and Poales lineage of commelinids. Based on the synonymous and nonsynonymous substitution rates, the fate of duplicated MusaWRKY genes was predicted to have undergone sub-functionalization in which the duplicated gene copies retain a subset of the ancestral gene function. Also, to understand the regulatory roles of MusaWRKY during a biotic stress, Illumina sequencing was performed on resistant and susceptible cultivars during the infection of root lesion nematode, Pratylenchus coffeae. The differential WRKY gene expression analysis in nematode resistant and susceptible cultivars during challenged and unchallenged conditions had distinguished: 1) MusaWRKYs participating in general banana defense mechanism against P.coffeae common to both susceptible and resistant cultivars, 2) MusaWRKYs that may aid in the pathogen survival as suppressors of plant triggered immunity, 3) MusaWRKYs that may aid in the host defense as activators of plant triggered immunity and 4) cultivar specific MusaWRKY regulation. Mainly, MusaWRKY52, -69 and -92 are found to be P.coffeae specific and can act as activators or repressors in a

  6. Evolutionary Expansion of WRKY Gene Family in Banana and Its Expression Profile during the Infection of Root Lesion Nematode, Pratylenchus coffeae.

    Directory of Open Access Journals (Sweden)

    Raja Kaliyappan

    Full Text Available The WRKY family of transcription factors orchestrate the reprogrammed expression of the complex network of defense genes at various biotic and abiotic stresses. Within the last 96 million years, three rounds of Musa polyploidization events had occurred from selective pressure causing duplication of MusaWRKYs with new activities. Here, we identified a total of 153 WRKY transcription factors available from the DH Pahang genome. Based on their phylogenetic relationship, the MusaWRKYs available with complete gene sequence were classified into the seven common WRKY sub-groups. Synteny analyses data revealed paralogous relationships, with 17 MusaWRKY gene pairs originating from the duplication events that had occurred within the Musa lineage. We also found 15 other MusaWRKY gene pairs originating from much older duplication events that had occurred along Arecales and Poales lineage of commelinids. Based on the synonymous and nonsynonymous substitution rates, the fate of duplicated MusaWRKY genes was predicted to have undergone sub-functionalization in which the duplicated gene copies retain a subset of the ancestral gene function. Also, to understand the regulatory roles of MusaWRKY during a biotic stress, Illumina sequencing was performed on resistant and susceptible cultivars during the infection of root lesion nematode, Pratylenchus coffeae. The differential WRKY gene expression analysis in nematode resistant and susceptible cultivars during challenged and unchallenged conditions had distinguished: 1 MusaWRKYs participating in general banana defense mechanism against P.coffeae common to both susceptible and resistant cultivars, 2 MusaWRKYs that may aid in the pathogen survival as suppressors of plant triggered immunity, 3 MusaWRKYs that may aid in the host defense as activators of plant triggered immunity and 4 cultivar specific MusaWRKY regulation. Mainly, MusaWRKY52, -69 and -92 are found to be P.coffeae specific and can act as activators or

  7. Bacillus species (BT42) isolated from Coffea arabica L. rhizosphere antagonizes Colletotrichum gloeosporioides and Fusarium oxysporum and also exhibits multiple plant growth promoting activity.

    Science.gov (United States)

    Kejela, Tekalign; Thakkar, Vasudev R; Thakor, Parth

    2016-11-18

    Colletotrichum and Fusarium species are among pathogenic fungi widely affecting Coffea arabica L., resulting in major yield loss. In the present study, we aimed to isolate bacteria from root rhizosphere of the same plant that is capable of antagonizing Colletotrichum gloeosporioides and Fusarium oxysporum as well as promotes plant growth. A total of 42 Bacillus species were isolated, one of the isolates named BT42 showed maximum radial mycelial growth inhibition against Colletotrichum gloeosporioides (78%) and Fusarium oxysporum (86%). BT42 increased germination of Coffee arabica L. seeds by 38.89%, decreased disease incidence due to infection of Colletotrichum gloeosporioides to 2.77% and due to infection of Fusarium oxysporum to 0 (p Fusarium oxysporum. The mechanism of action of inhibition of the pathogenic fungi found to be synergistic effects of secondary metabolites, lytic enzymes, and siderophores. The major inhibitory secondary metabolite identified as harmine (β-carboline alkaloids).

  8. Contribution to the study of 14C-acetate as the precursor of aminoacids in detached leaves of coffee (Coffea arabica cv. Mundo Novo)

    International Nuclear Information System (INIS)

    Brasil, O.G.

    1975-01-01

    Labelled acetates with 14 C were used as the forerunner of aminoacids in leaves of coffee (Coffea arabica cv Mundo Novo). Leaves with the labelled acetates were incubated and released CO 2 was retained in paper discs with hiamine for further radioactivity detection. Separated proteins furnished 13 amino-acids through acid hidrolysis, all of them were identified by bidimensional filter paper chromatography. Through the obtained results it is possible to conclude that acetates are metabolized by the leafs and are related to the processes of leaf synthesis. It was possible to show that an utilization of acetate for energetical production via Krebs cycle was donne. The obtained conclusions show too that methylic carbon was more incorporated than carboxylic carbon [pt

  9. Inoculación de Consorcios Micorrícicos Arbusculares en Coffea arabica, Variedad Caturra en la Región San Martín

    OpenAIRE

    Del Aguila, Karen M.; Vallejos-Torres, Geomar; Arévalo, Luis A.; Becerra, Alejandra G.

    2018-01-01

    Resumen El objetivo del presente estudio fue determinar el efecto de nueve consorcios micorrícicos arbusculares y la inoculación a plántulas de café Coffea arabica, variedad Caturra, comparados con un control (sin inoculación), durante siete meses en vivero. El cultivo de café en la Región San Martín es una de las actividades agrícolas económicas más importantes. Una alternativa para incrementar la producción consiste en la aplicación de hongos micorrizas. Se empleó un Diseño Completamente al...

  10. Effect of Maruca vitrata (Lepidoptera: Crambidae) host plants on life-history parameters of the parasitoid Apanteles taragamae (Hymenoptera: Braconidae)

    NARCIS (Netherlands)

    Dannon, A.E.; Tamo, M.; Agboton, C.; Huis, van A.; Dicke, M.

    2012-01-01

    The effect of four host plant species of the herbivore Maruca vitrata Fabricius (Lepidoptera: Crambidae) on development time, longevity, fecundity and sex ratio of the parasitoid Apanteles taragamae Viereck (Hymenoptera: Braconidae) was investigated under laboratory conditions. The larvae were

  11. Hyperparasitoid wasps (Hymenoptera: Trigonalidae) reared from dry forest and rain forest caterpillars of Area de Conservacion, Guanacaste, Costa Rica

    Science.gov (United States)

    Five species of Trigonalidae, hyperparasites of Ichneumonidae (Hymenoptera) and Tachinidae (Diptera) that parasitize caterpillars (Lepidoptera), have been reared during the ongoing caterpillar inventory of Area de Conservación Guanacaste (ACG), Guanacaste Province, northwestern Costa Rica: Lycogaste...

  12. Role of emerald ash borer (Coleoptera: Buprestidae) larval vibrations in host-quality assessments by Tetrastichus planipennisi (Hymenoptera: Eulophidae)

    Science.gov (United States)

    Michael D. Ulyshen; Richard W. Mankin; Yigen Chen; Jian J. Duan; Therese M. Poland; Leah S. Bauer

    2011-01-01

    The biological control agent Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) is a gregarious larval endoparasitoid of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive cambium-feeding species responsible for recent, widespread mortality of ash (Fraxinus spp.) in...

  13. Reproductive and developmental biology of the emerald ash borer parasitoid Spathius galinae (Hymenoptera: Braconidae) as affected by temperature

    Science.gov (United States)

    Emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is an invasive pest of serious concern in North America. To complement ongoing biological control efforts, Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a recently-described specialist parasitoid of ...

  14. α-Amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits α-amylases from the coffee berry borer pest

    Directory of Open Access Journals (Sweden)

    Oliveira-Neto Osmundo B

    2010-06-01

    Full Text Available Abstract Background Coffee is an important crop and is crucial to the economy of many developing countries, generating around US$70 billion per year. There are 115 species in the Coffea genus, but only two, C. arabica and C. canephora, are commercially cultivated. Coffee plants are attacked by many pathogens and insect-pests, which affect not only the production of coffee but also its grain quality, reducing the commercial value of the product. The main insect-pest, the coffee berry borer (Hypotheneumus hampei, is responsible for worldwide annual losses of around US$500 million. The coffee berry borer exclusively damages the coffee berries, and it is mainly controlled by organochlorine insecticides that are both toxic and carcinogenic. Unfortunately, natural resistance in the genus Coffea to H. hampei has not been documented. To overcome these problems, biotechnological strategies can be used to introduce an α-amylase inhibitor gene (α-AI1, which confers resistance against the coffee berry borer insect-pest, into C. arabica plants. Results We transformed C. arabica with the α-amylase inhibitor-1 gene (α-AI1 from the common bean, Phaseolus vulgaris, under control of the seed-specific phytohemagglutinin promoter (PHA-L. The presence of the α-AI1 gene in six regenerated transgenic T1 coffee plants was identified by PCR and Southern blotting. Immunoblotting and ELISA experiments using antibodies against α-AI1 inhibitor showed a maximum α-AI1 concentration of 0.29% in crude seed extracts. Inhibitory in vitro assays of the α-AI1 protein against H. hampei α-amylases in transgenic seed extracts showed up to 88% inhibition of enzyme activity. Conclusions This is the first report showing the production of transgenic coffee plants with the biotechnological potential to control the coffee berry borer, the most important insect-pest of crop coffee.

  15. Alpha-amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits alpha-amylases from the coffee berry borer pest.

    Science.gov (United States)

    Barbosa, Aulus E A D; Albuquerque, Erika V S; Silva, Maria C M; Souza, Djair S L; Oliveira-Neto, Osmundo B; Valencia, Arnubio; Rocha, Thales L; Grossi-de-Sa, Maria F

    2010-06-17

    Coffee is an important crop and is crucial to the economy of many developing countries, generating around US$70 billion per year. There are 115 species in the Coffea genus, but only two, C. arabica and C. canephora, are commercially cultivated. Coffee plants are attacked by many pathogens and insect-pests, which affect not only the production of coffee but also its grain quality, reducing the commercial value of the product. The main insect-pest, the coffee berry borer (Hypotheneumus hampei), is responsible for worldwide annual losses of around US$500 million. The coffee berry borer exclusively damages the coffee berries, and it is mainly controlled by organochlorine insecticides that are both toxic and carcinogenic. Unfortunately, natural resistance in the genus Coffea to H. hampei has not been documented. To overcome these problems, biotechnological strategies can be used to introduce an alpha-amylase inhibitor gene (alpha-AI1), which confers resistance against the coffee berry borer insect-pest, into C. arabica plants. We transformed C. arabica with the alpha-amylase inhibitor-1 gene (alpha-AI1) from the common bean, Phaseolus vulgaris, under control of the seed-specific phytohemagglutinin promoter (PHA-L). The presence of the alpha-AI1 gene in six regenerated transgenic T1 coffee plants was identified by PCR and Southern blotting. Immunoblotting and ELISA experiments using antibodies against alpha-AI1 inhibitor showed a maximum alpha-AI1 concentration of 0.29% in crude seed extracts. Inhibitory in vitro assays of the alpha-AI1 protein against H. hampei alpha-amylases in transgenic seed extracts showed up to 88% inhibition of enzyme activity. This is the first report showing the production of transgenic coffee plants with the biotechnological potential to control the coffee berry borer, the most important insect-pest of crop coffee.

  16. The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1 and Bacillus subtilis (UB_Bs1 on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae

    Directory of Open Access Journals (Sweden)

    Presti Mardiyani Purwaningtyas

    2016-11-01

    Full Text Available Parasitic Root-Lession nematode of Pratylenchus coffeae can reduce the Indonesian coffee plants productivity. Several studies reported that Pseudomonas fluorescens and Bacillus subtilis endophytic bacteria were antagonistic bacteria to nematode. The objective of this research was to reveal the effectiveness of bacterial colonies density of P. fluorescens (UB_Pf1, B.subtilis (UB BS1, and a combination of both bacteria on nematode mortality using median lethal concentration (LC50 and median lethal time 50 (LT50. The densities of bacteria used in this study were 107, 109, 1011 and 1013 cfu/ml. 35 testing nematodes were used and the mortality was counted at 6, 12, 24, 36, and 48 hours after treatments. The results showed that LC50 values of P. fluorescens was (UB_Pf1 was 4,3x108 cfu/ml, LC50 B. subtilis (UB_Bs1 was 1,9x109cfu/ ml, and LC50 combination of both bacteria was, 8x107 cfu/ml. It implies that the application of the combination of both bacteria are more pathogenic than single bacterial treatment. The results also showed that the highest LT50 value was 13.21  hours combination of bacterial colonies with a density of 1013 cfu/ml and the lowest LT50 value was 52.00 hours on P. fluorescens (UB_Pf1 treatment with colonies density of 107 cfu/ml.How to CitePurwaningtyas, P. M., Rahardjo, B. T., & Tarno, H. (2016. The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1 and Bacillus subtilis (UB_Bs1 on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae. Biosaintifika: Journal of Biology & Biology Education, 8(3, 286-293. 

  17. First record of Aleiodes laphygmae (Hymenoptera: Braconidae) for Argentina and its association with larvae of Spodoptera eridania (Lepidoptera: Noctuidae)

    OpenAIRE

    Valverde, Liliana; Berta, D. Carolina; Gomez, Marcelo Geronimo

    2012-01-01

    Se reporta por primera vez para Argentina Aleiodes laphygmae (Viereck) (Hymenoptera: Braconidae) y como parasitoide de larvas de Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae), en plantaciones de soja en la provincia de Tucumán (Argentina). Se provee información biológica como hábitos, hospedadores y distribución.Aleiodes laphygmae (Viereck) (Hymenoptera: Braconidae), is reported for the first time for Argentina. It is also reported parasitizing larvae of Spodoptera eridania (Stoll) (Le...

  18. Bumble bee (Hymenoptera: Apidae) community structure on two sagebrush steppe sites in southern Idaho

    Science.gov (United States)

    Stephen P. Cook; Sara M. Birch; Frank W. Merickel; Carrie Caselton Lowe; Deborah Page-Dumroese

    2011-01-01

    Although sagebrush, Artemisia spp., does not require an insect pollinator, there are several native species of bumble bees, Bombus spp. (Hymenoptera: Apidae), that are present in sagebrush steppe ecosystems where they act as pollinators for various forbs and shrubs. These native pollinators contribute to plant productivity and reproduction. We captured 12 species of...

  19. The species of the genus Hypodynerus de Saussure (Hymenoptera, Vespidae, Eumeninae occurring in Brazil

    Directory of Open Access Journals (Sweden)

    Bolívar Garcete-Barrett

    2013-04-01

    Full Text Available An identification table and descriptions are given to recognize the two species of Hypodynerus (Hymenoptera: Vespidae: Eumeninae recorded from Brazil: H. arechavaletae (Brèthes and H. duckei (Bertoni comb. n. The lectotype is designated and the male is described for Hypodynerus duckei, its presence being recorded from Brazil for the first time.

  20. The species of the genus Hypodynerus de Saussure (Hymenoptera, Vespidae, Eumeninae) occurring in Brazil.

    Science.gov (United States)

    Garcete-Barrett, Bolívar R; Hermes, Marcel Gustavo

    2013-01-01

    An identification table and descriptions are given to recognize the two species of Hypodynerus (Hymenoptera: Vespidae: Eumeninae) recorded from Brazil: Hypodynerus arechavaletae (Brèthes) and Hypodynerus duckei (Bertoni) comb. n. The lectotype is designated and the male is described for Hypodynerus duckei, its presence being recorded from Brazil for the first time.

  1. [Prevalence of hymenoptera sting allergy in veterinary medicine students from Monterey, Nuevo Leon, Mexico].

    Science.gov (United States)

    Arias Cruz, Alfredo; Monsiváis Toscano, Gina; Gallardo Martínez, Gabriela; González Díaz, Sandra Nora; Galindo Rodríguez, Gabriela

    2007-01-01

    The reported prevalence of allergic systemic reactions to hymenoptera venom occur in up to 3.3% and large local reactions occur in 17% in the general population. To investigate the prevalence of hymenoptera sting allergy in a group of veterinary medicine students from Monterrey, Nuevo Leon, Mexico. A transverse and observational study was done with 64 students of veterinary medicine. We conducted a questionnaire about the students' history of insect allergy and atopy. Skin test with allergenic extracts of bee and ant were practiced to all subjects. We performed aeroallergen skin prick test to the subjets with suspected atopy. Students age ranged from 17 to 25 years (mean 20.2) and 37 were males. Twenty students (31.3%) had clinical history of atopy and positive skin tests to aeroallergens. On the other hand, 5 students (7.8%), including 2 atopic, had suffered large local reactions, but none of them had suffered systemic reactions. Bee and ant skin tests were positive in 15.6% and 31.3% of the students respectively. There was no difference in the prevalence of hymenoptera allergy between atopic and non atopic subjects (p < 0.05). Further, the frequency of atopy in subjects with positive skin tests for bee and ant was 50%. The prevalence of large local reactions and hymenoptera sensitization found in this group was similar to that found in other epidemiologic studies.

  2. First report of Eurytoma plotnikovi Nik. (Hymenoptera, Eurytomidae, a seed parasite of pistachio, in Sicily (Italy

    Directory of Open Access Journals (Sweden)

    Santi Longo

    2011-09-01

    Full Text Available The pistachio seed wasp, Eurytoma plotnikovi Nik.(Hymenoptera, E urytomidae, is a new pest recently arrived in pistachio orchards in central-western Sicily (Italy. Information on the damaging effects of this seed wasp in the affected areas is provided.

  3. Invasive ant Tapinoma melanocephalum (Hymenoptera: Formicidae): A rare quest or increasingly common indoor pest in Europe?

    Czech Academy of Sciences Publication Activity Database

    Klimeš, Petr; Okrouhlík, Jan

    2015-01-01

    Roč. 112, č. 4 (2015), s. 705-712 ISSN 1210-5759 R&D Projects: GA ČR GB14-36098G Institutional support: RVO:60077344 Keywords : Hymenoptera * Formicidae * Tapinoma Subject RIV: EH - Ecology, Behaviour Impact factor: 0.975, year: 2014 http://www.eje.cz/pdfs/eje/2015/04/16.pdf

  4. The species of the Neotropical genus Fractipons Townes, 1970 (Hymenoptera, Ichneumonidae, Cryptinae).

    Science.gov (United States)

    Bordera, Santiago; González-Moreno, Alejandra

    2011-01-19

    In this paper, two new species of the Neotropical genus Fractipons Townes, 1970 (Hymenoptera, Ichneumonidae) are described. A new diagnosis for the genus, a re-description of Fractipons cincticornis Townes, 1970 and a key to known species are provided. New distribution records for the genus now include Argentina, Costa Rica, Panama and Peru.

  5. DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini)

    OpenAIRE

    Rocha, MP; Pompolo, SD; Dergam, JA; Fernandes, A; Campos, LAD

    2002-01-01

    We analyzed patterns of heterochromatic bands in the Neotropical stingless bee genus Melipona (Hymenoptera, Meliponini). Group I species (Melipona bicolor bicolor, Melipona quadrifasciata, Melipona asilvae, Melipona marginata, Melipona subnitida) were characterized by low heterochromatic content. Group 11 species (Melipona capixaba, Melipona compressipes, Melipona crinita, Melipona seminigra fuscopilosa e Melipona scutellaris) had high heterochromatic content. All species had 2n = 18 and n = ...

  6. The Hymenopterous Poison Apparatus. X. Morphological and Behavioral Changes in Atta texana (Hymenoptera: Formicidae)

    Science.gov (United States)

    Henry R. Hermann; John C. Moser; Allen N. Hunt

    1970-01-01

    Atta texana (Buckley) and other members of this genus no longer utilize the 8th and 9th gonapophyses as part of their defensive system. Although the sclerites that comprise the stinging apparatus in most aculeate Hymenoptera are present in the species, they seem to function only in the deposition of trail pheromones. A mechanical and chemical defense...

  7. Epidemiology, Diagnosis, and Treatment of Hymenoptera Venom Allergy in Mastocytosis Patients

    NARCIS (Netherlands)

    Niedoszytko, Marek; Bonadonna, Patrizia; Oude Elberink, Joanne N. G.; Golden, David B. K.

    Hymenoptera venom allergy is a typical IgE-mediated reaction caused by sensitization to 1 or more allergens of the venom, and accounts for 1.5% to 34% of all cases of anaphylaxis. Patients suffering from mastocytosis are more susceptible to the anaphylactic reactions to an insect sting. This article

  8. Application of recombinant antigen 5 allergens from seven allergy-relevant Hymenoptera species in diagnostics

    DEFF Research Database (Denmark)

    Schiener, Maximilian; Eberlein, Bernadette; Moreno Aguilar, Carmen

    2017-01-01

    BACKGROUND: Hymenoptera stings can cause severe anaphylaxis in untreated venom-allergic patients. A correct diagnosis regarding the relevant species for immunotherapy is often hampered by clinically irrelevant cross-reactivity. In vespid venom allergy, cross-reactivity between venoms of different...

  9. The spatial distribution of Hymenoptera parasitoids in a forest reserve in Central Amazonia, Manaus, AM, Brazil

    Directory of Open Access Journals (Sweden)

    RB. Querino

    Full Text Available Parasitoids are of great importance to forest ecosystems due to their ecological role in the regulation of the population of other insects. The species richness and abundance of parasitoids in the forest canopy and understory, both on the borders and in the interior of a tropical forest reserve in Central Amazonia were investigated. For a 12-month period, specimen collections were made every 15 days from suspended traps placed in the forest canopy and in the understory strata, both on the border and in the interior of forest areas. A total of 12,835 Hymenoptera parasitoids from 23 families were acquired. Braconidae, Diapriidae, Mymaridae, Eulophidae, and Scelionidae were the most represented in the area and strata samples. The results indicate that there were no significant differences in the species richness or abundance of Hymenoptera between the forest borders and the inner forest. The data does show that the presence of Hymenoptera is significantly greater in the understory in both the border and interior areas than in the canopy (vertical stratification. Aphelinidae and Ceraphronidae were significantly associated with the inner forest, while the other seven families with the border of the reserve. The abundance of Hymenoptera parasitoids presented seasonal variations during the year related to the rainy and dry seasons.

  10. Revision of the subfamily Euphorinae (excluding the tribe Meteorini Cresson) (Hymenoptera: Braconidae) from China

    NARCIS (Netherlands)

    Chen, X.; Achterberg, van C.

    1997-01-01

    The subfamily Euphorinae (excluding the tribe Meteorini Cresson) (Hymenoptera: Braconidae) from China is revised. In total 150 species, belonging to 24 genera, are treated and keyed. One genus (Heia gen. nov.; type species: Heia robustipes spec. nov.) and 69 species are described as new to science.

  11. Stiff upper lip: Labrum deformity and functionality in bees (Hymenoptera: Apoidea)

    Science.gov (United States)

    In hyper-diverse groups such as Hymenoptera, a variety of structures with different, complementary functions are used for feeding. Although the function of the parts such as the mandibles is obvious, the use of others, like the labrum, is more difficult to discern. Here, we discuss the labrum’s func...

  12. De kortsnuitbloedbij Sphecodes majalis nieuw voor de Nederlandse fauna (Hymenoptera: Apidae)

    NARCIS (Netherlands)

    Raemakers, I.

    2004-01-01

    Sphecodes majalis, a new bee species for the Netherlands (Hymenoptera: Apidae) A population of Sphecodes majalis was found on a limestone grassland near Maastricht (Limburg). On several occasions more than 10 female and several male specimen were observed. Sphecodes majalis is a parasite of

  13. De roodrandzandbij Andrena rosae in de Zuid-Hollandse Biesbosch (Hymenoptera: Apoidea: Andrenidae).

    NARCIS (Netherlands)

    Meer, van der F.; Reemer, M.; Peeters, T.M.J.

    2006-01-01

    Andrena rosae in the Zuid-Hollandse Biesbosch (Hymenoptera: Apoidea: Andrenidae) Andrena rosae is a fairly large, black and red mining bee. It has declined dramatically in the 20th century in northwestern Europe. In the Netherlands the species only survived in and around the Biesbosch, at the

  14. Revision of charipine aphid hyperparasitoids (Hymenoptera, Cynipoidea: Figitidae) from central Europe

    Czech Academy of Sciences Publication Activity Database

    Ferrer-Suay, M.; Starý, Petr; Selfa, J.; Pujade-Villar, J.

    2017-01-01

    Roč. 28, č. 3 (2017), s. 113-147 ISSN 0785-8760 Institutional support: RVO:60077344 Keywords : Hymenoptera * central Europe * aphid Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.300, year: 2016 http://www.entomologicafennica.org/Volume28/EF_28_3/1Ferrer-Suay.pdf

  15. Absorção, translocação e utilização de zinco, cobre e manganês por mudas enxertadas de Coffea arabica Absorption, translocation and utilization of zinc, copper and manganese in grafted seedlings of Coffea arabica

    Directory of Open Access Journals (Sweden)

    Marcelo Antonio Tomaz

    2006-04-01

    Full Text Available Comparativamente ao pé-franco, a combinação enxerto/porta-enxerto altera os complexos mecanismos de "feedback" entre parte aérea e raízes, afetando de maneira positiva ou negativa a eficiência nutricional da planta. Este trabalho teve como objetivo avaliar, em cultivo hidropônico, a eficiência da absorção, translocação e utilização de Zn, Cu e Mn por mudas de Coffea arabica L., de acordo com o porta-enxerto utilizado. O experimento foi realizado em casa de vegetação, por um período de 170 dias, em vasos que continham areia como substrato, recebendo solução nutritiva circulante. Utilizaram-se, como enxerto, quatro genótipos de C. arabica: os cultivares Catuaí Vermelho IAC 15 e Oeiras MG 6851 e os híbridos 'H 419-10-3-1-5' e 'H 514-5-5-3' , e, como porta-enxerto, quatro genótipos, sendo três de Coffea canephora Pierre ex Froenher: Apoatã LC 2258, Conilon Muriaé-1 e RC EMCAPA 8141 (recombinação entre clones da variedade Robustão Capixaba - EMCAPA 8141 e uma linhagem de Coffea arabica L.: Mundo Novo IAC 376-4, além de quatro pés-francos. O delineamento experimental utilizado foi em blocos casualizados com 20 tratamentos, quatro repetições e uma planta por parcela. A eficiência nutricional das mudas quanto ao Zn, Cu e Mn variou de acordo com a combinação enxerto/porta-enxerto. A progênie 'H 514-5-5-3' foi mais eficiente quanto à utilização de Zn, Cu e Mn e produção de matéria seca, quando combinada com os porta-enxertos Apoatã LC 2258 e Mundo Novo IAC 376-4. O Catuaí Vermelho IAC 15 foi mais eficiente na utilização de Cu e Mn quando combinado com Apoatã LC 2258.There are complexes feedback mechanisms involving the relationship rootstock/scion, whick can positively or negatively affect the plant nutrient efficiency use. The goal of the present work was to evaluate the efficiency of uptake, translocation and utilization of Zn, Cu and Mn in coffee seedlings. The experiment was carried out in a greenhouse

  16. Etude de l’hybridation inter-spécifique entre espèces du genre Coffea en Nouvelle-Calédonie : distribution des niches favorables et structuration de la diversité

    OpenAIRE

    Gomez , Céline

    2009-01-01

    Coffee trees are originated from inter-tropical forests of Africa and Madagascar and their diversification origin areas are highly threatened. Among the 103 species of the Coffea genus, only three are cultivated: C. arabica, C. canephora and C. liberica. C. canephora has the widest natural distribution in tropical African forests, and its genetic diversity is structured in five distinct groups. The study of the genetic diversity structuring has revealed a particular importance of the Dahomey ...

  17. Effects of gamma radiation of Cobalt-60 on arabica and conillon seeds coffea: physic-chemistry evaluation; Efeitos da radiacao gama do Cobalto-60 em sementes de cafe arabica e conillon: avaliacao fisico-quimica

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Marcus Henriques da

    2012-07-01

    Brazil is the largest producer and exporter of coffee in the world. The coffee bean is one of the main products of the Brazilian trade balance. Two species of coffee are the most economically important: the Coffea arabica L. and Coffea canephora Pierre is the largest representative of the Coffea canephora Pierre is the coffea conillon. Food irradiation is an area of research that aims to increase the shelf life of foods and controlling pests. This study aimed to verify the physicochemical variables of Arabica coffee and conillon were affected when exposed to doses of gamma radiation from cobalt-60. The samples were provided by Polo in Coffee Quality Technology, Federal University of Lavras - UFLA. The coffee samples were subjected to irradiation doses: 0 (control), 5 kGy and 10 kGy, a multipurpose irradiator of IPEN - Research Institute of Nuclear Energy and the University of São Paulo, at a rate of 7.5 kGy / hour. For irradiation the samples were vacuum-packed in appropriate packaging aluminised. After the process of irradiation the samples were stored at a temperature of 15 ± 1 deg C and relative humidity of 17 ± 1%. The following analyzes were performed: levels of total sugars, glucose, sucrose, caffeine, humidity, pH, total acidity, electrical conductivity and fibers. Analyses were performed 1, 30, 60 and 90 days after irradiation, and the results were submitted to analysis of variance and means were compared by Tukey test at 5%. It was observed that the analysis results of the samples irradiated with 5 kGy and 10 kGy showed values similar to the control. It was concluded that irradiation did not induce deleterious effects on arabica coffee seeds and conillon irradiated with 5 kGy and 10 kGy to 90 days after irradiation. (author)

  18. Sting microsculpture in the digger wasp Bembix rostrata (Hymenoptera, Crabronidae

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    Natalia Matushkina

    2011-03-01

    Full Text Available The sting microsculpture of the digger wasp Bembix rostrata (Fabricius, 1781 (Hymenoptera, Crabronidae is studied with the scanning electron microscope (SEM for the first time. As in many other hymenopterans, the second valvifer of B. rostrata possesses two fields of styloconic sensilla (hair plates of proprioceptive function. The presence of two paired fields of campaniform sensilla on the second valvula and second valvifer is first shown in an apoid wasp. The first and the second valvulae bear scattered sensilla-like structures on the external surface, more numerous apically. The first valvula has two subapical barbs externally and a pair of valvilli on its inner surface, whereas the outer surface of the second valvula is smooth. The third valvula is sclerotized externally, consisting of proximal and distal parts, and bearing four sensilla morphotypes of mechanoreceptive and probably chemoreceptive functions. The inner surface of the valvulae and the membranous cuticle that is touching the sting have microstructures of different shapes directed distally. Functional aspects of characters studied are discussed.

  19. Function and evolution of microRNAs in eusocial Hymenoptera

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    Eirik eSovik

    2015-05-01

    Full Text Available The emergence of eusociality (true sociality in several insect lineages represents one of the most successful evolutionary adaptations in the animal kingdom in terms of species richness and global biomass. In contrast to solitary insects, eusocial insects evolved a set of unique behavioral and physiological traits such as reproductive division of labor and cooperative brood care, which likely played a major role in their ecological success. The molecular mechanisms that support the social regulation of behavior in eusocial insects, and their evolution, are mostly unknown. The recent whole-genome sequencing of several eusocial insect species set the stage for deciphering the molecular and genetic bases of eusociality, and the possible evolutionary modifications that led to it. Studies of mRNA expression patterns in the brains of diverse eusocial insect species have indicated that specific social behavioral states of individual workers and queens are often associated with particular tissue-specific transcriptional profiles. Here we discuss recent findings that highlight the role of non-coding microRNAs (miRNAs in modulating traits associated with reproductive and behavioral divisions of labor in eusocial insects. We provide bioinformatic and phylogenetic data, which suggest that some Hymenoptera-specific miRNA may have contributed to the evolution of traits important for the evolution of eusociality in this group.

  20. Foraging strategies of the ant Ectatomma vizottoi (Hymenoptera, Formicidae

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    Luan D. Lima

    2013-12-01

    Full Text Available Foraging strategies of the ant Ectatomma vizottoi (Hymenoptera, Formicidae. Foraging activity may be limited by temperature, humidity, radiation, wind, and other abiotic factors, all of which can affect energy costs during foraging. Ectatomma vizottoi's biology has only recently been studied, and no detailed information is available on its foraging patterns or diet in the field. For this reason, and because foraging activity is an important part of the ecological success of social insects, the present study aimed to investigate E. vizottoi's foraging strategies and dietary habits. First, we determined how abiotic factors constrained E. vizottoi's foraging patterns in the field by monitoring the foraging activity of 16 colonies on eight different days across two seasons. Second, we characterized E. vizottoi's diet by monitoring another set of 26 colonies during peak foraging activity. Our results show that E. vizottoi has foraging strategies that are similar to those of congeneric species. In spite of having a low efficiency index, colonies adopted strategies that allowed them to successfully obtain food resources while avoiding adverse conditions. These strategies included preying on other ant species, a foraging tactic that could arise if a wide variety of food items are not available in the environment or if E. vizottoi simply prefers, regardless of resource availability, to prey on other invertebrates and especially on other ant species.

  1. Preservation of Domesticated Honey Bee (Hymenoptera: Apidae) Drone Semen.

    Science.gov (United States)

    Paillard, M; Rousseau, A; Giovenazzo, P; Bailey, J L

    2017-08-01

    Preservation of honey bee (Apis mellifera L., Hymenoptera: Apidae) sperm, coupled with instrumental insemination, is an effective strategy to protect the species and their genetic diversity. Our overall objective is to develop a method of drone semen preservation; therefore, two experiments were conducted. Hypothesis 1 was that cryopreservation (-196 °C) of drone semen is more effective for long-term storage than at 16 °C. Our results show that after 1 yr of storage, frozen sperm viability was higher than at 16 °C, showing that cryopreservation is necessary to conserve semen. However, the cryoprotectant used for drone sperm freezing, dimethyl sulfoxide (DMSO), can harm the queen and reduce fertility after instrumental insemination. Hypothesis 2 was that centrifugation of cryopreserved semen to reduce DMSO prior to insemination optimize sperm quality. Our results indicate that centrifuging cryopreserved sperm to remove cryoprotectant does not affect queen survival, spermathecal sperm count, or sperm viability. Although these data do not indicate that centrifugation of frozen-thawed sperm improves queen health and fertility after instrumental insemination, we demonstrate that cryopreservation is achievable, and it is better for long-term sperm storage than above-freezing temperatures for duration of close to a year. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  2. Wolbachia in two populations of Melittobia digitata Dahms (Hymenoptera: Eulophidae)

    International Nuclear Information System (INIS)

    Copeland, Claudia S.; Sivinski, John

    2008-01-01

    We investigated two populations of Melittobia digitata Dahms, a gregarious parasitoid (primarily upon a wide range of solitary bees, wasps, and flies), in search of Wolbachia infection. The first population, from Xalapa, Mexico, was originally collected from and reared on Mexican fruit fly pupae, Anastrepha ludens Loew (Diptera: Tephritidae); the other, from Athens, Georgia, was collected from and reared on prepupae of mud dauber wasps, Trypoxylon politum Say (Hymenoptera: Crabronidae). PCR studies of the ITS2 region corroborated that both parasitoid populations were the same species; this potentially provides a useful molecular taxonomic profile since females of Melittobia species are superficially similar. Amplification of the Wolbachia surface protein gene (wsp) confirmed the presence of this endosymbiont in both populations. Sequencing revealed that the Wolbachia harbored in both populations exhibited a wsp belonging to a unique subgroup (denoted here as Dig) within the B-supergroup of known wsp genes. This new subgroup of wsp may either belong to a different strain of Wolbachia from those previously found to infect Melittobia or may be the result of a recombination event. In either case, known hosts of Wolbachia with a wsp of this subgroup are only distantly related taxonomically. Reasons are advanced as to why Melittobia - an easily reared and managed parasitoid - holds promise as an instructive model organism of Wolbachia infection amenable to the investigation of Wolbachia strains among its diverse hosts. (author)

  3. Wolbachia in two populations of Melittobia digitata Dahms (Hymenoptera: Eulophidae)

    Energy Technology Data Exchange (ETDEWEB)

    Copeland, Claudia S.; Sivinski, John [United States Dept. of Agriculture, Gainesville, FL (United States). Center for Medical, Agriculture and Veterinary Entomology]. E-mails: cclaudia@bioinf.uni-leipzig.de; john.sivinski@ars.usda.gov; Matthews, Robert W. [University of Georgia, Athens, GA (United States). Dept. of Entomology]. E-mail: rmatthew@uga.edu; Gonzalez, Jorge M. [Texas A and M Univ., College Station, TX (United States). Dept. of Entomology]. E-mail: jmgonzalez@neo.tamu.edu; Aluja, Martin [Instituto de Ecologia A.C., Veracruz (Mexico)]. E-mail: martin.aluja@inecol.edu.mx

    2008-11-15

    We investigated two populations of Melittobia digitata Dahms, a gregarious parasitoid (primarily upon a wide range of solitary bees, wasps, and flies), in search of Wolbachia infection. The first population, from Xalapa, Mexico, was originally collected from and reared on Mexican fruit fly pupae, Anastrepha ludens Loew (Diptera: Tephritidae); the other, from Athens, Georgia, was collected from and reared on prepupae of mud dauber wasps, Trypoxylon politum Say (Hymenoptera: Crabronidae). PCR studies of the ITS2 region corroborated that both parasitoid populations were the same species; this potentially provides a useful molecular taxonomic profile since females of Melittobia species are superficially similar. Amplification of the Wolbachia surface protein gene (wsp) confirmed the presence of this endosymbiont in both populations. Sequencing revealed that the Wolbachia harbored in both populations exhibited a wsp belonging to a unique subgroup (denoted here as Dig) within the B-supergroup of known wsp genes. This new subgroup of wsp may either belong to a different strain of Wolbachia from those previously found to infect Melittobia or may be the result of a recombination event. In either case, known hosts of Wolbachia with a wsp of this subgroup are only distantly related taxonomically. Reasons are advanced as to why Melittobia - an easily reared and managed parasitoid - holds promise as an instructive model organism of Wolbachia infection amenable to the investigation of Wolbachia strains among its diverse hosts. (author)

  4. Atrativo para as abelhas Apis mellifera e polinização em café (Coffea arabica L.

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    Darclet Teresinha Malerbo-Souza

    2003-01-01

    Full Text Available O presente experimento foi conduzido em Jaboticabal, SP, e teve como objetivos estudar uma cultura de café (Coffea arabica L., var. Mundo Novo, quanto à biologia floral, a freqüência e comportamento dos insetos na flor, testar o produto Bee-HereR (Hoescht Shering Agrevo do Brasil Ltda quanto a sua atratividade para as abelhas Apis mellifera e verificar a produção de frutos com e sem a visita dos insetos. Para isso, foram verificados o tempo do desenvolvimento e quantidade de açúcar solúvel do néctar das flores; freqüência das visitações dos insetos, no decorrer do dia, por meio de contagem do número de insetos visitando as flores, a cada 60 minutos, das 8 às 17 horas, 10 minutos em cada horário; tempo (em segundos e tipo de coleta (néctar e/ou pólen dos insetos mais freqüentes; perda de botões florais; porcentagem de flores que se transformaram em frutos; tempo de formação e contagem dos grãos de café, observando-se a porcentagem de frutificação em flores visitadas ou não pelos insetos. Também foram realizados testes por pulverização utilizando-se o produto Bee-HereR , diluído em xarope e em água, em diferentes horários. A flor durou, em média, cerca de 3 dias desde sua abertura até o murchamento. A quantidade de açúcares do néctar apresentou diferença significativa entre os horários, sendo maior às 8 horas (em média, 102,18 ± 8,75 mg de carboidratos totais por flor. A abelha A. mellifera foi o inseto mais freqüente nas flores de café, coletando, principalmente, néctar no decorrer do dia. A perda de botões florais causada pelas chuvas foi, em média, 26,50 ± 11,70%. O tempo para a formação do fruto foi 6 meses e o número de frutos decorrentes do tratamento descoberto foi maior (38,79% e 168,38%, em 1993 e 1994, respectivamente que do tratamento coberto. Apesar da eficiência do produto Bee-HereR ser afetada pelas condições climáticas, ele pode ser usado para atrair as abelhas A. mellifera na

  5. Eventos do processo de infecção de Colletotrichum gloeosporioides inoculados em folhas de Coffea arabica L

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    Josimar Batista Ferreira

    2009-12-01

    Full Text Available O presente trabalho teve por objetivo obter informações sobre os eventos de pré-penetração, penetração e colonização de isolados de C. gloeosporioides, obtidos de mangueira e cafeeiro, quando inoculados em folhas de cafeeiros (Coffea arabica L. da cultivar Catucaí Vermelho. As folhas foram selecionadas, padronizadas e lavadas, demarcando-se áreas circulares de 0,5 cm de diâmetro na face abaxial, inoculando-se uma alíquota de 20 µL da suspensão de conídios. Utilizou-se um isolado obtido de mangueira e dois isolados obtidos de cafeeiro com mancha manteigosa. Realizaram-se avaliações com 3, 6, 8, 12, 24, 36, 48, 72, 96, 144 e 240 horas após a inoculação (hai. Todos os materiais foram processados e observados em microscópico eletrônico de varredura. Os conídios de todos os isolados aderiram freqüentemente nas depressões das células da epiderme e células-guarda dos estômatos, formando septo antes da germinação. A penetração, na maior parte, se deu por via direta e algumas vezes por estômatos. Isolados de cafeeiro germinaram em folhas de 6 a 8 hai, produzindo apressórios 12 hai e acérvulos de 96 a 144 hai. O isolado de mangueira germinou de 6 a 8 hai com formação de apressório de 8 a 12 hai e produziu novos conídios diretamente em hifas conidiogênicas. Não foi observada a formação acérvulos para este isolado.The present study was carried out with the objective to provide information on the pre-penetration, penetration and colonization events of isolates of Colletotrichum gloeosporioides from mango and coffee, when inoculated in coffee leaves. The cultivar Catucaí Vermelho was used in all experiments. Coffee leaves were selected, standardized and washed in sterile water. After that, circular areas of 0.5 cm in diameter were marked on the undersurface of the leaves. In the center of each circular area, 20 µL of spore suspension was deposited. One isolate from mango and two from coffee presenting blister

  6. Coffea canephora Pierre

    African Journals Online (AJOL)

    Uganda has been hampered by logistics. These clonal ... confirmed that it is highly profitable to apply nitrogen to robusta coffee. The differences ..... cost of in yield over due to response profit"'. /ha/year {kg}"1. Nitrogen zero level {kg) to ...

  7. Epidemiological study of the prevalence of allergic reactions to Hymenoptera in a rural population in the Mediterranean area.

    Science.gov (United States)

    Fernandez, J; Blanca, M; Soriano, V; Sanchez, J; Juarez, C

    1999-08-01

    Systemic allergic reactions to Hymenoptera venom occur in a percentage that varies from 0.4 to 3.3%. Epidemiological studies indicate that from 15 to 25% of the general population can be sensitized to different Hymenoptera venom as well as the fact that the degree of exposure may be related to the prevalence found in those studies. The objective of this study was to evaluate the prevalence of insect sting allergy and the venom sensitization in a rural population to three Hymenoptera previously found in the area: Polistes dominulus (Pd), Vespula germanica (Vg) and honey bee (Hb). A rural community located in the south-east of Spain, close to the Mediterranean Sea, was selected since the stinging Hymenoptera having been previously identified. A random sample of 310 subjects from the village census was studied. A questionnaire and a serum sample were obtained from every patient. The evaluation was conducted by a family doctor, who focused on the reactions to Hymenoptera sting, age, sex, occupation, atopia, previous Hymenoptera sting, stinging insect, interval to last sting and average stings per year. RAST to Hymenoptera venoms were also determined. The prevalence of systemic reactions was 2.3% (57.6% of them had a positive RAST). Large local reactions were found in 26.4% (only 28.5% of them had a positive RAST). Asymptomatic sensitization (positive RAST) was observed in 16.4% of subjects without reaction. Only a weak correlation between subjects with less than 3 years' interval to last sting exposure and positive RAST results was noted, whether they presented with a clinical reaction or not (P < 0.05). The prevalence of systemic sting reactions in our rural community is higher than other general populations in the same Mediterranean area, and similar to other rural populations studied. The degree of exposure influences not only the prevalence found but also the detection of specific serum IgE.

  8. Effect of 6-BA on nodal explant bud sproutings of Coffea arabica cv. Mundo Novo Efeito de 6-BA na brotação de gemas de explantes nodais de Coffea arabica cv. Mundo Novo

    Directory of Open Access Journals (Sweden)

    Luis Carlos da Silva Ramos

    2005-01-01

    Full Text Available Coffee plants can be micropropagated by nodal bud sprouting using the 6-benzylaminopurine (6-BA hormone. However, literature reports the use of a wide range of 6-BA, from 0.5 to 88.8 µM L-1. So, this study was performed to narrow that range. Nodal explants of Coffea arabica cv Mundo Novo obtained from in vitro plantlets were inoculated on gelled-MS medium supplemented with different concentrations of 6-BA. Two assays were carried out: in the first one, 6-BA was used at concentrations of 0, 5, 25, 50, and 100 µM L-1, being evaluated at 43 and 123 days. In the second experiment, dosis of 10, 20 and 30 µM L-1, have evaluated at 65 and 100 days. Treatments with 6-BA induced multiple sprouting from the nodal explants, which were best characterized around 100 days after inoculation. The nodal explants grew taller and showed multiple shoots, whereas the effect of 6-BA at 5 to 25 µM L-1 was similar to that with higher concentrations (50 and 100 µM L-1. Nodal explants yielded from 2.9 to 6.0 buds per node, achieving height of 1.3 to 1.5 cm at 5 to 25 µM L-1 of 6-BA, whereas they yielded from 4.3 to 4.9 buds per node but the sprouting grew about 0.8 cm at 50 and 100 µM L-1 of 6-BA. This study indicated that multiple sprouting of lateral buds can be induced by lower concentrations of 6-BA, for example, from 10 to 30 µM L-1, diminishing possible risks of somaclonal variation due to high levels of hormone concentration.O cafeeiro pode ser micropropagado via brotação de gemas laterais, aplicando o regulador de crescimento 6-benzilaminopurina (6-BA. Entretanto, a literatura apresenta ampla variação da dose empregada, desde 0.5 a 88.8 µM L-1. Assim, este estudo visou otimizar doses para explantes nodais do cafeeiro C. arabica cv Mundo Novo. Explantes nodais, obtidos de plântulas cultivadas in vitro, foram inoculados em meio MS geleificado, com adição de diferentes concentrações de 6-BA. Foram feitos dois experimentos: no primeiro, 6-BA foi

  9. Nesting biology of Centris (Hemisiella tarsata Smith (Hymenoptera, Apidae, Centridini

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    Cândida M. L. Aguiar

    2004-09-01

    Full Text Available Nests of Centris tarsata Smith, 1874 were obtained from trap-nests in areas of dry semi-deciduous forest (Baixa Grande and caatinga (Ipirá, in the State of Bahia. Nesting occurred in bamboo canes and in tubes of black cardboard with 5.8 cm (= small tube and 10.5 cm (= large tube in length and 0.6 and 0.8 cm in diameter, respectively. In both areas C. tarsata nested during the wet season producing four generations in Baixa Grande and three generations in Ipirá. The immatures of one generation underwent diapause at both sites. The bees constructed their nests with a mixture of sand and oil. In general, the cells were elongated and arranged in linear series with its opening pointing towards the nest entrance. Completed nests had two to three cells in small tubes, one to seven cells in large tubes, and two to 13 cells in bamboo canes. The nest plug resembled an uncompleted cell and was externally covered with oil. The cells were provisioned with pollen, oil, and nectar. Nests were parasitized by Mesocheira bicolor (Fabricius, 1804 (Hymenoptera: Apidae and other not identify bee species.Ninhos de Centris tarsata Smith, 1874 foram obtidos através da utilização de ninhos-armadilha, em áreas de floresta estacional semi-decídua (Baixa Grande e de caatinga (Ipirá, no Estado da Bahia. A nidificação ocorreu em gomos de bambus e em tubos de cartolina preta, estes com comprimentos de 5,8 cm (= tubos pequenos e 10,5 cm (= tubos grandes, e diâmetro de 0,6 e 0,8 cm, respectivamente. Em ambas as áreas C. tarsata nidificou durante a estação úmida, produzindo quatro gerações anuais em Baixa Grande e três em Ipirá. Os imaturos de uma das gerações passaram por diapausa em ambos os locais. As abelhas construíram seus ninhos com uma mistura de areia e óleo. Em geral, as células foram alongadas e arranjadas em série linear, com sua abertura dirigida para a entrada do ninho. Os ninhos completados tinham de duas a três células nos tubos pequenos

  10. Fertility signals in the bumblebee Bombus terrestris (Hymenoptera: Apidae)

    Science.gov (United States)

    Sramkova, A.; Schulz, C.; Twele, R.; Francke, W.; Ayasse, M.

    2008-06-01

    In eusocial Hymenoptera, queen control over workers is probably inseparable from the mechanism of queen recognition. In primitively eusocial bumblebees ( Bombus), worker reproduction is controlled not only by the presence or absence of a dominant queen but also by other dominant workers. Furthermore, it was shown that the queen dominance is maintained by pheromonal cues. We investigated whether there is a similar odor signal released by egg-laying queens and workers that may have a function as a fertility signal. We collected cuticular surface extracts from nest-searching and breeding Bombus terrestris queens and workers that were characterized by their ovarian stages. In chemical analyses, we identified 61 compounds consisting of aldehydes, alkanes, alkenes, and fatty acid esters. Nest-searching queens and all groups of breeding females differed significantly in their odor bouquets. Furthermore, workers before the competition point (time point of colony development where workers start to develop ovaries and lay eggs) differed largely from queens and all other groups of workers. Breeding queens showed a unique bouquet of chemical compounds and certain queen-specific compounds, and the differences toward workers decrease with an increasing development of the workers’ ovaries, hinting the presence of a reliable fertility signal. Among the worker groups, the smallest differences were found after the competition point. Egg-laying females contained higher total amounts of chemical compounds and of relative proportions of wax-type esters and aldehydes than nest-searching queens and workers before the competition point. Therefore, these compounds may have a function as a fertility signal present in queens and workers.

  11. Simplification of intradermal skin testing in Hymenoptera venom allergic children.

    Science.gov (United States)

    Cichocka-Jarosz, Ewa; Stobiecki, Marcin; Brzyski, Piotr; Rogatko, Iwona; Nittner-Marszalska, Marita; Sztefko, Krystyna; Czarnobilska, Ewa; Lis, Grzegorz; Nowak-Węgrzyn, Anna

    2017-03-01

    The direct comparison between children and adults with Hymenoptera venom anaphylaxis (HVA) has never been extensively reported. Severe HVA with IgE-documented mechanism is the recommendation for venom immunotherapy, regardless of age. To determine the differences in the basic diagnostic profile between children and adults with severe HVA and its practical implications. We reviewed the medical records of 91 children and 121 adults. Bee venom allergy was exposure dependent, regardless of age (P bee venom allergic group, specific IgE levels were significantly higher in children (29.5 kU A /L; interquartile range, 11.30-66.30 kU A /L) compared with adults (5.10 kU A /L; interquartile range, 2.03-8.30 kU A /L) (P venom were higher in bee venom allergic children compared with the wasp venom allergic children (P venom. At concentrations lower than 0.1 μg/mL, 16% of wasp venom allergic children and 39% of bee venom allergic children had positive intradermal test results. The median tryptase level was significantly higher in adults than in children for the entire study group (P = .002), as well as in bee (P = .002) and wasp venom allergic groups (P = .049). The basic diagnostic profile in severe HVA reactors is age dependent. Lower skin test reactivity to culprit venom in children may have practical application in starting the intradermal test procedure with higher venom concentrations. Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  12. Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae

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    Vladimir Gokhman

    2011-08-01

    Full Text Available Drosophila melanogaster Meigen, 1830 has served as a model insect for over a century. Sequencing of the 11 additional Drosophila Fallen, 1823 species marks substantial progress in comparative genomics of this genus. By comparison, practically nothing is known about the genome size or genome sequences of parasitic wasps of Drosophila. Here, we present the first comparative analysis of genome size and karyotype structures of Drosophila parasitoids of the Leptopilina Förster, 1869 and Ganaspis Förster, 1869 species. The gametic genome size of Ganaspis xanthopoda (Ashmead, 1896 is larger than those of the three Leptopilina species studied. The genome sizes of all parasitic wasps studied here are also larger than those known for all Drosophila species. Surprisingly, genome sizes of these Drosophila parasitoids exceed the average value known for all previously studied Hymenoptera. The haploid chromosome number of both Leptopilina heterotoma (Thomson, 1862 and L. victoriae Nordlander, 1980 is ten. A chromosomal fusion appears to have produced a distinct karyotype for L. boulardi (Barbotin, Carton et Keiner-Pillault, 1979 (n = 9, whose genome size is smaller than that of wasps of the L. heterotoma clade. Like L. boulardi, the haploid chromosome number for G. xanthopoda is also nine. Our studies reveal a positive, but non linear, correlation between the genome size and total chromosome length in Drosophila parasitoids. These Drosophila parasitoids differ widely in their host range, and utilize different infection strategies to overcome host defense. Their comparative genomics, in relation to their exceptionally well-characterized hosts, will prove to be valuable for understanding the molecular basis of the host-parasite arms race and how such mechanisms shape the genetic structures of insect communities.

  13. Thermoperiodism Synchronizes Emergence in the Alfalfa Leafcutting Bee (Hymenoptera: Megachilidae).

    Science.gov (United States)

    Yocum, George D; Rinehart, Joseph P; Yocum, Ian S; Kemp, William P; Greenlee, Kendra J

    2016-02-01

    Alfalfa seed production in the northwestern United States and western Canada is heavily dependent upon the pollinating services of Megachile rotundata (F.) (Hymenoptera: Megachilidae). M. rotundata females nest in cavities either naturally occurring or in artificial nesting blocks. Because of the physical nature of the nest, M. rotundata brood may have limited to no exposure to photoperiodic cues in order to regulate important circadian functions. Therefore, various thermoperiod regimes were used to characterize the possible role of thermoperiodism in synchronizing M. rotundata adult emergence. Adult emergence was monitored using a microprocessor-controlled event logger. Incubating bees under constant 29°C and darkness resulted in an arhythmic adult emergence pattern. Exposing developing M. rotundata to a thermoperiod synchronized emergence to the beginning of the thermophase and decreased the total number of days required for all adults to emerge. The amplitude of the thermoperiod regulated the timing of peak emergence in relationship to the increase in temperature. A thermoperiod amplitude of only 2°C was sufficient to synchronize peak adult emergence to take place during the rise in temperature. Increasing the amplitude of the thermoperiod to 4 or 8°C caused a positively correlated shift in peak emergence to later in the thermophase. Brood stored under constant 29°C and darkness for different durations (May or June early in the growing season or July or August late in the growing season) or under a fluctuating thermal regime (base temperature of 6°C and daily 1-h pulse of 20°C until September or November) maintained their capacity for entraining emergence timing by thermoperiodism. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  14. Transferência do fator caturra para o cultivar Mundo Novo de Coffea arabica Transfer of the CT gene to Mundo Novo cultivar

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    A. Carvalho

    1972-01-01

    Full Text Available No presente trabalho são relatados os estudos realizados visando à introdução do gene Ct (caturra que contribui para reduzir a altura da planta, no cultivar Mundo" Novo de Coffea arabica.Estudaram-se, em ensaios de produtividade, as populações Fv F.,, F3 e F4. Nessas populações e principalmente entre os descendentes dos "caféeiros H 2077-2-5 e H 2077-2-12, foram selecionadas plantas homozigotas para os alelos Ct e também para os alelos responsáveis pela cor do fruto xc ou Xc. Essas combinações foram denominadas 'Catuaí Amarelo' e 'Catuaí Vermelho', respectivamente, e suas características são apresentadas. Os novos cultivares vêm-se mostrando de interesse econômico para as regiões cafeeiras não somente pelo porte pequeno, mas também pela produtividade, pelo vigor vegetativo e pela precocidade.The successful transfer of the Ct gene for short internode to the tall cultivar of Coffea arábica'Mundo Novo' is reported. Individual selections were carried out in the F1, F2, F3 and F4 generations. It was found that early selection in the F2 generation was quite effective. A remarkably good correlation was found between productitivity of F2 plants and the yield of the F3 and F4 generations. Plants of the F4 generation have shown reasonable uniformity and high yield in several trials. The new selections showed to be early producers. Two new cultivars were released namely 'Catuaí Amarelo' and 'Catuaí Vermelho'. The former has yellow fruits whereas the latter has red fruits. The plants are much shorter that the ones of Mundo Novo. The new cultivars have a very strong secondary and tertiary branching. Because of these characteristics Catuaí Amarelo and Catuaí Vermelho are being planted in large scale replacing the tall cultivars.

  15. Los Chalcidoidea (Hymenoptera asociados con agallas de Cinípidos (Hymenoptera, Cynipidae en la Comunidad de Madrid

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    Gómez, J. F.

    2006-05-01

    Full Text Available Information about the chalcid wasp parasitoid community (Hymenoptera, Chalcidoidea associated with galls of Cynipidae in Madrid (Spain is compiled and updated. Studied material includes more than 1000 published and unpublished records from samplings in 80 sites in the Madrid region carried out over twenty three years by the research team. A check-list of 121 species, 19 of them provisionally identified, from 6 families of Chalcidoidea is provided as follows: 26 Eurytomidae, 27 Torymidae, 9 Ormyridae, 33 Pteromalidae, 9 Eupelmidae and 17 Eulophidae. Ormyrus rufimanus Mayr, 1904 and Idiomacromerus semiaeneus (Szelenyi, 1957 are recorded for the first time in the Iberian Peninsula. For each family and genus of Chalcidoidea data are given on biology, diversity and distribution in Comunidad de Madrid. The composition of the chalcid wasp parasitoid community associated with gall wasps in Comunidad de Madrid is discussed and compared to the same community data from the Iberian Peninsula and the Western Palaearctic. Species from the Iberian community of chalcid parasitoids that are missing from Madrid region, exclusive species and potentially present species are also commented upon. Finally two appendices are presented as follows: a list of the Chalcidoidea species reared from 73 different galls made by 71 cynipid species from Madrid with associated parasitoid records, and a final appendix with full record data of all studied material, including information on the sampling localities with X, Y georeferenced coordinates, host galls and host plants.

    Se compila y actualiza la información existente sobre la comunidad parasitoide de Chalcidoidea, asociada a agallas de cinípidos (Hymenoptera, Cynipidae en la Comunidad de Madrid. Los datos corresponden a más de 1000 registros, tanto de datos publicados, como inéditos, correspondientes a colectas en 80 localidades de Madrid efectuadas por el equipo investigador a lo largo de 23 a

  16. Alterações morfofisiológicas em folhas de Coffea arabica L. cv. "Oeiras" sob influência do sombreamento por Acacia mangium Willd Morphophysiological alterations in leaves of Coffea arabica L. cv. 'Oeiras' shaded by Acacia mangium Willd

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    Inês Angélica Cordeiro Gomes

    2008-02-01

    Full Text Available Diferenças na disponibilidade de radiação podem causar modificações na estrutura e função das folhas do cafeeiro, que podem responder de maneira diferencial à radiação por alterações morfológicas, anatômicas, de crescimento e na taxa fotossintética. O objetivo deste trabalho foi avaliar características morfofisiológicas de cafeeiros (Coffea arabica L. cv. "Oeiras" sombreados por acácia (Acacia mangium Willd. na época seca e chuvosa no sul de Minas Gerais. As maiores taxas fotossintéticas e maiores espessuras da epiderme adaxial foram observadas na estação chuvosa nas linhas de cafeeiros a pleno sol. O sombreamento influenciou em menor espessura das folhas e em espaços intercelulares maiores no tecido esponjoso. Foi também verificada mudança na forma dos cloroplastos, os quais apresentaram-se mais alongados em folhas de cafeeiros a pleno sol quando relacionados aos arborizados.Light availability is one of the most important environmental factors affecting leaf structure and functions in coffee plants that can respond differently to radiation by changes in leaf anatomy, morphology, growth and photosynthetic rate. The objective of this research was evaluate some morphophysiological aspects in leaves of coffee (Coffea arabica L. cv. 'Oeiras' cropped under shelter trees in the south of Minas Gerais during the rainy and dry season. The shade caused lower leaves thickness and higher intercellular spaces in spongious tissue. There was also verified a change in chloroplast shape, which showed more elongated in coffee tree kept at full sunlight in relation to that ones maintained on shading.

  17. Interações entre auxinas e ácido bórico, no enraizamento de estacas caulinares de Coffea arabica L. cv. Mundo Novo Interactions between auxins and boric acid in the rooting of stem cuttings Coffea arabica L. cv. "Mundo Novo"

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    E.O. Ono

    1992-01-01

    Full Text Available O presente trabalho teve como finalidade, estudar o efeito de auxinas e do boro no enraizamento de estacas caulinares de Coffea arabica L. cv. "Mundo Novo". As estacas foram retiradas de ramos ortotrópicos semi-lenhosos de cafeeiro, as quais foram tratadas durante 24 horas com soluções de IBA ou NAA e boro, e a mistura das três substâncias, resultando um total de 14 tratamentos. Para a avaliação do objetivo em questão, foram realizadas as seguintes observações, mediante coleta após 90 dias de plantio: número de estacas enraizadas e número de estacas com calos. Através dos resultados obtidos, pode-se concluir que, para obter um maior número de estacas enraizadas, é conveniente o tratamento com NAA à 100 ou 200 ppm mais boro.The present research had as purpose to study auxin and boron effects on rooting of Coffea arábica L. cv. "Mundo Novo" stem cuttings. The cuttings were taken from orthotropous semi-hardwood branches of coffee-tree, which were treated during 24 hours with IBA or NAA and boron solutions, and the mixture of the three substances, resulting a total of 14 treatments. The following observations were realized, taking the cuttings 90 days after planting: number of rooted cuttings and "callus" formation per cutting. It can be concluded that to obtain a higher number of rooted cuttings, the treatment with NAA at 100 or 200 ppm plus boron is the most suitable.

  18. Teste de condutividade elétrica individual na avaliação da qualidade fisiológica de sementes de café (Coffea arabica L. Individual electrical conductivity test for evaluation of the physiological quality of coffee seeds (Coffea arabica L.

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    Paula de Souza Cabral Costa

    2006-02-01

    Full Text Available O teste de condutividade elétrica é usado para medir os exsudatos das sementes, que certamente refletem a integridade do sistema de membranas. O teste é rápido e de operação simples, mas a metodologia necessita ser testada para cada espécie individualmente para obter uma melhor precisão e exatidão dos resultados. Este trabalho foi conduzido visando determinar o ponto de partição na realização do teste de condutividade elétrica em sementes de café. Dez lotes de sementes de Coffea arabica sem pergaminho, cultivar Acaiá foram usados nesse estudo. Para a avaliação do ponto de partição as sementes foram embebidas por 96 horas e submetidas ao teste de germinação, correlacionando os valores de condutividade observados com os valores obtidos no teste de germinação. O ponto de partição é de 120,5µS.cm-1, considerando 70% de germinação.The conductivity test is used to measure the leaches from the seeds, which certaintily reflect the membrane system integrity. The test is rapid and it is of simple operation, but the methodology needs to be tested for each individual species in order to reach a better precision and accuracy. This work was conducted to determinate the partition point for the realization of electrical conductivity test in coffee seeds. Ten seed lots of Coffea arabica, cultivar Acaiá, without parchment, were used in this study. For the evaluation of the partition point, seeds were imbibed for 96 hours and submitted to the germination test, with the correlation between the conductivity values and those obtained in the germination test being evaluated. The partition point is 120,5µS.cm-1, considering 70% of germination.

  19. Partial cytochrome b sequences for six Hymenoptera of the eastern United States.

    Science.gov (United States)

    Collins, A M; Gardner, L M

    2001-01-01

    Mitochondrial DNA (mtDNA) haplotypes have been commonly used to determine honeybee subspecies relationships. To see if these markers would also be useful for comparisons of other Hymenoptera, we collected workers of six local species: Vespa crabro, the European hornet; Bombus impatiens, a bumblebee; Vespula germanica, the German yellow jacket; Polistes fuscatus, a paper wasp; Halictus ligatus, an alkali bee; and an unspecified Megachile, a leafcutting bee. MtDNA was isolated and digested with six endonucleases (AvaI, BglII, EcoRI, HindIII, HinfI, XbaI). The digested DNA was electrophoresed and visualized on agarose gels with comparison to a standard fragment marker and similarly treated honeybee mtDNA. The fragments obtained were also purified and sequenced. Phylogenetic relationships between six wasp and bee species, Apis mellifera, and several other similar aculeate Hymenoptera were determined. Newly defined DNA sequences were posted to GenBank (AF281169-AF281174).

  20. Ichneumonidae (Hymenoptera) parasitóides de larvas de Lepidoptera associadas a Croton floribundus Spreng (Euphorbiaceae)

    OpenAIRE

    Fernandes, Luciana Bueno dos Reis; Dias Filho, Manoel Martins; Fernandes, Marcelo Adorna; Penteado-Dias, Angelica Maria

    2010-01-01

    Parasitoids of the family Ichneumonidae (Hymenoptera) were obtained during an inventory of Lepidoptera larvae caught feeding in the wild on Croton floribundus (Euphorbiaceae). The Lepidoptera larvae were collected from host plants along trails inside three preserved forest areas in the Brazilian state of São Paulo. Fifteen different species of Ichneumonidae belonging to five subfamilies (Banchinae, Campopleginae, Cremastinae, Mesochorinae and Metopiinae) were obtained. Seven species of Ichneu...

  1. A Gynandromorph and Teratological Case in Spilomicrus sp. (Hymenoptera, Diaprioidea, Diapriidae

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    Emerson Comério

    2015-12-01

    Resumo. Este estudo relata a ocorrência de antenas com características femininas e masculinas em um exemplar fêmea de Spilomicrus sp. (Hymenoptera, Diaprioidea, Diapriidae coletado no Parque Estadual Intervales, Ribeirão Grande, São Paulo, Brasil, assim como malformações em uma das antenas, que são aqui descritas e ilustradas.

  2. Morphology and function of the ovipositor mechanism in Ceraphronoidea (Hymenoptera, Apocrita

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    Andrew Ernst

    2013-08-01

    Full Text Available The ovipositor of apocritan Hymenoptera is an invaluable source of phylogenetically relevant characters, and our understanding of its functional morphology stands to enlighten us about parasitoid life history strategies. Although Ceraphronoidea is one of the most commonly collected Hymenoptera taxa with considerable economic importance, our knowledge about their natural history and phylogenetic relationships, both to other apocritan lineages and within the superfamily itself, is limited. As a first step towards revealing ceraphronoid natural diversity we describe the skeletomuscular system of the ceraphronoid ovipositor for the first time. Dissections and Confocal Laser Scanning Microscopy 3D media files were used to visualize the ovipositor complex and to develop character concepts. Morphological structures were described in natural language and then translated into a character-character state format, whose terminology was linked to phenotype-relevant ontologies. Four unique anatomical phenotypes were revealed: 1. The first valvifer (gonangulum of the genus Trassedia is composed of two articulating sclerites, a condition present only in a few basal insect taxa. The bipartition of the first valvifer in Trassedia is most likely secondary and might allow more rapid oviposition. 2. Ceraphronoids, unlike other Hymenoptera, lack the retractor muscle of the terebra; instead the egg laying device is retracted by the seventh sternite. 3. Also unlike other Hymenoptera, the cordate apodeme and the anterior flange of the second valvifer are fused and compose one ridge that serves as the site of attachment for the dorsal and ventral T9-second valvifer muscles. Overall, the ceraphronoid ovipositor system is highly variable and can be described by discrete, distinguishable character states. However, these differences, despite their discrete nature, do not reflect the present classification of the superfamily and might represent parallelisms driven by host

  3. Identification of microsatellite markers for a worldwide distibuted, highly invasive ant species Tapinoma melanocephalum (Hymenoptera: Formicidiae)

    Czech Academy of Sciences Publication Activity Database

    Zima, Jan; Lebrasseur, O.; Borovanská, Michaela; Janda, Milan

    2016-01-01

    Roč. 113, JUNE 03 (2016), s. 409-414 E-ISSN 1802-8829 R&D Projects: GA ČR GAP505/12/2467 Grant - others:Marie Curie Fellowship(CZ) PIOFGA2009-25448 Institutional support: RVO:60077344 Keywords : Hymenoptera * Formicidae * Tapinoma melanocephalum Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.167, year: 2016 http://www.eje.cz/pdfs/eje/2016/01/53.pdf

  4. Accelerated evolution of mitochondrial but not nuclear genomes of Hymenoptera: new evidence from crabronid wasps.

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    Martin Kaltenpoth

    Full Text Available Mitochondrial genes in animals are especially useful as molecular markers for the reconstruction of phylogenies among closely related taxa, due to the generally high substitution rates. Several insect orders, notably Hymenoptera and Phthiraptera, show exceptionally high rates of mitochondrial molecular evolution, which has been attributed to the parasitic lifestyle of current or ancestral members of these taxa. Parasitism has been hypothesized to entail frequent population bottlenecks that increase rates of molecular evolution by reducing the efficiency of purifying selection. This effect should result in elevated substitution rates of both nuclear and mitochondrial genes, but to date no extensive comparative study has tested this hypothesis in insects. Here we report the mitochondrial genome of a crabronid wasp, the European beewolf (Philanthus triangulum, Hymenoptera, Crabronidae, and we use it to compare evolutionary rates among the four largest holometabolous insect orders (Coleoptera, Diptera, Hymenoptera, Lepidoptera based on phylogenies reconstructed with whole mitochondrial genomes as well as four single-copy nuclear genes (18S rRNA, arginine kinase, wingless, phosphoenolpyruvate carboxykinase. The mt-genome of P. triangulum is 16,029 bp in size with a mean A+T content of 83.6%, and it encodes the 37 genes typically found in arthropod mt genomes (13 protein-coding, 22 tRNA, and two rRNA genes. Five translocations of tRNA genes were discovered relative to the putative ancestral genome arrangement in insects, and the unusual start codon TTG was predicted for cox2. Phylogenetic analyses revealed significantly longer branches leading to the apocritan Hymenoptera as well as the Orussoidea, to a lesser extent the Cephoidea, and, possibly, the Tenthredinoidea than any of the other holometabolous insect orders for all mitochondrial but none of the four nuclear genes tested. Thus, our results suggest that the ancestral parasitic lifestyle of

  5. Phylogeny and population genetic structure of ant genus Acropyga (Hymenoptera: Formicidae) in Papua New Guinea

    Czech Academy of Sciences Publication Activity Database

    Janda, Milan; Matos Maravi, Pavel F.; Borovanská, Michaela; Zima, Jan; Youngerman, E.; Pierce, N. E.

    2016-01-01

    Roč. 30, č. 1 (2016), s. 28-40 ISSN 1445-5226 R&D Projects: GA ČR GAP505/12/2467 Grant - others:Marie Curie Fellowship(CZ) PIOFGA2009-25448; Operational Program Research and Development for Innovations(CZ) CZ.1.05/3.2.00/08.0144 Institutional support: RVO:60077344 Keywords : Acropyga * Hymenoptera * Papua New Guinea Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.172, year: 2016

  6. Meteorus arizonensis Muesebeck, 1923 (Hymenoptera: Braconidae): nuevo registro para México

    OpenAIRE

    Gutiérrez-Ramírez, A.; Robles-Bermúdez, A.; Cambero-Campos, J.; Coronado-Blanco, J. M.

    2015-01-01

    Braconidae is one of the more diverse families of Hymenoptera with almost 20,000 species worldwide. Meteorus has 316 species and is almost cosmopolitan. Previously, seven species of this genus have been recorded from Mexico. In this note, Meteorus arizo-nensis Muesebeck is recorded for the first time for the country, obtained from the fall armyworm, Spodoptera frugiperda (J.E. Smith), with material from Santa Maria del Oro, locality in the State of Nayarit.

  7. Parasitoidism of Chalcidid wasps (Hymenoptera, Chalcididae on Philornis sp. (Diptera, Muscidae

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    M. S. Couri

    Full Text Available Philornis Meinert larvae are known as parasites of birds, with coprophagous, semi-hematophagous or hematophagous habits. Biological data of the larvae of the fifty described species are still scarcely known. Here we describe some aspects of the parasitism of a species of Philornis on Thalurania glaucopis Gmelin (Trochilidae and record two species of Chalcididae (Hymenoptera parasitoids, Conura annulifera (Walker, 1864 and Brachymeria podagrica (Fabricius, 1787, reared from Philornis puparia.

  8. Can the Understory Affect the Hymenoptera Parasitoids in a Eucalyptus Plantation?

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    Onice Teresinha Dall'Oglio

    Full Text Available The understory in forest plantations can increase richness and diversity of natural enemies due to greater plant species richness. The objective of this study was to test the hypothesis that the presence of the understory and climatic season in the region (wet or dry can increase the richness and abundance of Hymenoptera parasitoids in Eucalyptus plantations, in the municipality of Belo Oriente, Minas Gerais State, Brazil. In each eucalyptus cultivation (five areas of cultivation ten Malaise traps were installed, five with the understory and five without it. A total of 9,639 individuals from 30 families of the Hymenoptera parasitoids were collected, with Mymaridae, Scelionidae, Encyrtidae and Braconidae being the most collected ones with 4,934, 1,212, 619 and 612 individuals, respectively. The eucalyptus stands with and without the understory showed percentage of individuals 45.65% and 54.35% collected, respectively. The understory did not represent a positive effect on the overall abundance of the individuals Hymenoptera in the E. grandis stands, but rather exerted a positive effect on the specific families of the parasitoids of this order.

  9. Can the Understory Affect the Hymenoptera Parasitoids in a Eucalyptus Plantation?

    Science.gov (United States)

    Dall’Oglio, Onice Teresinha; Ribeiro, Rafael Coelho; Ramalho, Francisco de Souza; Fernandes, Flávio Lemes; Wilcken, Carlos Frederico; de Assis Júnior, Sebastião Lourenço; Rueda, Rosa Angélica Plata; Serrão, José Eduardo; Zanuncio, José Cola

    2016-01-01

    The understory in forest plantations can increase richness and diversity of natural enemies due to greater plant species richness. The objective of this study was to test the hypothesis that the presence of the understory and climatic season in the region (wet or dry) can increase the richness and abundance of Hymenoptera parasitoids in Eucalyptus plantations, in the municipality of Belo Oriente, Minas Gerais State, Brazil. In each eucalyptus cultivation (five areas of cultivation) ten Malaise traps were installed, five with the understory and five without it. A total of 9,639 individuals from 30 families of the Hymenoptera parasitoids were collected, with Mymaridae, Scelionidae, Encyrtidae and Braconidae being the most collected ones with 4,934, 1,212, 619 and 612 individuals, respectively. The eucalyptus stands with and without the understory showed percentage of individuals 45.65% and 54.35% collected, respectively. The understory did not represent a positive effect on the overall abundance of the individuals Hymenoptera in the E. grandis stands, but rather exerted a positive effect on the specific families of the parasitoids of this order. PMID:26954578

  10. [Establisment of a genetic transformation method of coffee (Coffea arabica cv. Catimor) and incorporation of bar gene for ammonium glufosinate resistance].

    Science.gov (United States)

    Fernández Da Silva, Rafael

    2003-01-01

    In order to establish a successful method of genetic transformation in Coffea arabica cv. Catimor, different conditions of generation and electroporation were evaluated on different plant tissues. Cell suspension system was improved using one hormone only (BA), obtaining high yields of primary and secondary somatic embryo production. For selection of viable and potentially transformed cells, MTT (1%) method and ammonium glufosinate concentration (1 mg/L in leaf, callus and embryos; and 5 mg/L in cells) were established. Different conditions were evaluated to electroporate different explants (embryogenic callus, vitroplants leaves, globular and torpedo embryos). The highest gus gene expression percentage by explant were found on enzymatic treated tissues at 375 V/cm in callus, and at 625 V/cm in leaves and embryos. Torpedo embryos cultured on liquid medium were the only type of tissue that could regenerate into plants, where secondary somatic embryos were obtained. Those embryos were positive to the gus gene histochemical test and to the gus and bar genes amplification on a PCR reaction.

  11. Purine alkaloid formation and CO2 gas exchange in dependence of development and of environmental factors in leaves of Coffea arabica L.

    Science.gov (United States)

    Frischknecht, P M; Eller, B M; Baumann, T W

    1982-12-01

    In the leaves of Coffea arabica L., purine alkaloid formation was estimated by analyzing the theobromine and caffeine content and by measuring the methylation rate of [2-(14)C]theobromine to [2-(14)C]caffeine in short-term experiments (6-24 h). At the same time, growth (in terms of dry weight and area), net photosynthesis (NPS), and dark respiration were determined. During leaf development, which was considered to be terminated when NPS was at a maximum (60-80 μmol g(-1) s(-1)) and dark respiration at a minimum (5-7.5 μmol g(-1) s(-1)), the content of theobromine and the velocity of caffeine formation were both found to decrease by a factor of more than 100. The close correlation between the theobromine content and the methylation rate is suspended when purine alkaloid formation is influenced by factors other than leaf development. Among these factors, temperature is the most effective: the velocity of caffeine biosynthesis is increased by raising the temperature and vice versa. Although the plants were well irrigated, a drastic decrease of NPS in the afternoon was observed under all environmental conditions tested. Light saturation was reached between 170-360 μmol m(-2) s(-1). The temperature optimum of NPS was shown to be very broad (24-33°C)m provided the adaptation time was sufficiently long.

  12. High-throughput metabolic profiling of diverse green Coffea arabica beans identified tryptophan as a universal discrimination factor for immature beans.

    Science.gov (United States)

    Setoyama, Daiki; Iwasa, Keiko; Seta, Harumichi; Shimizu, Hiroaki; Fujimura, Yoshinori; Miura, Daisuke; Wariishi, Hiroyuki; Nagai, Chifumi; Nakahara, Koichi

    2013-01-01

    The maturity of green coffee beans is the most influential determinant of the quality and flavor of the resultant coffee beverage. However, the chemical compounds that can be used to discriminate the maturity of the beans remain uncharacterized. We herein analyzed four distinct stages of maturity (immature, semi-mature, mature and overripe) of nine different varieties of green Coffea arabica beans hand-harvested from a single experimental field in Hawaii. After developing a high-throughput experimental system for sample preparation and liquid chromatography-mass spectrometry (LC-MS) measurement, we applied metabolic profiling, integrated with chemometric techniques, to explore the relationship between the metabolome and maturity of the sample in a non-biased way. For the multivariate statistical analyses, a partial least square (PLS) regression model was successfully created, which allowed us to accurately predict the maturity of the beans based on the metabolomic information. As a result, tryptophan was identified to be the best contributor to the regression model; the relative MS intensity of tryptophan was higher in immature beans than in those after the semi-mature stages in all arabica varieties investigated, demonstrating a universal discrimination factor for diverse arabica beans. Therefore, typtophan, either alone or together with other metabolites, may be utilized for traders as an assessment standard when purchasing qualified trading green arabica bean products. Furthermore, our results suggest that the tryptophan metabolism may be tightly linked to the development of coffee cherries and/or beans.

  13. Cuantificación de minerales K, Ca, Mg y P en pulpa y pergamino de café (Coffea arabica L. var. Typica

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    Johanna Mendoza Abarca

    2010-01-01

    Full Text Available Se trabajó con muestras de café Coffea arabica L., variedad Typica de tres lugares en Ecuador: Palanda, Vilcabamba y El Pangui, seleccionados por su disponibilidad y condiciones geográficas. Se analizó potasio, calcio y magnesio por espectrofotometría de absorción atómica; y fósforo por espectrofotometría visible, utilizando en todos los casos la digestión ácida para la preparación de las muestras. Con el objetivo de mantener la trazabilidad de los residuos se realizó el beneficio húmedo del café. Los resultados obtenidos para la pulpa de café expresados en base seca fueron: K 3,1 ± 0,43 %; Ca 0,46 ± 0,06 %; Mg 0,14 ± 0,01 % y P 0,13 ± 0,01 %. Para pergamino de café: K 0,16 ± 0,02 %; Ca 0,14 ± 0,05 %; Mg 0,06 ± 0,01 % y P 0,02 ± 0,01 %. En adición, se determinaron los minerales en el grano de café. Con estos resultados podemos mencionar que la pulpa de café es una buena fuente de potasio (especialmente, magnesio y fósforo. El pergamino pudiera ser considerado como fuente de fósforo.

  14. A liquid chromatography/electrospray ionisation tandem mass spectrometry method for the simultaneous quantification of salicylic, jasmonic and abscisic acids in Coffea arabica leaves.

    Science.gov (United States)

    de Sá, Marta; Ferreira, João P; Queiroz, Vagner T; Vilas-Boas, Luís; Silva, Maria C; Almeida, Maria H; Guerra-Guimarães, Leonor; Bronze, Maria R

    2014-02-01

    Plants have developed an efficient system of recognition that induces a complex network of signalling molecules such as salicylic acid (SA), jasmonic acid (JA) and abscisic acid (ABA) in case of a pathogenic infection. The use of specific and sensitive methods is mandatory for the analysis of compounds in these complex samples. In this study a liquid chromatography/electrospray ionisation tandem mass spectrometry method was developed and validated for the simultaneous quantification of SA, JA and ABA in Coffea arabica (L.) leaves in order to understand the role of these phytohormones in the signalling network involved in the coffee defence response against Hemileia vastatrix. The results showed that the method was specific, linear (r ≥ 0.99) in the range 0.125-1.00 µg mL⁻¹ for JA and ABA and 0.125-5.00 µg mL⁻¹ for SA, and precise (relative standard deviation ≤11%), and the limit of detection (0.010 µg g⁻¹ fresh weight) was adequate for quantifying these phytohormones in this type of matrix. In comparison with healthy leaves, those infected with H. vastatrix (resistance reaction) displayed an increase in SA level 24 h after inoculation, suggesting the involvement of an SA-dependent pathway in coffee resistance. © 2013 Society of Chemical Industry.

  15. Evaluation of biocontrol ability of native strains of Trichoderma spp on Rhizoctonia and Fusarium sp in coffee (Coffea arabica in experimental conditions

    Directory of Open Access Journals (Sweden)

    Nina Rudy

    2016-06-01

    Full Text Available Due to the indiscriminate use agrochemicals in conventional agriculture, it is causing pollution problems in the environment (soil, air and water, hence the search for alternatives that contribute to agricultural production by agro-chemical free sustainable production. This paper studies the biological control of damping off in coffee (Coffea arabica by applying antagonistic fungus Trichoderma sp. Under experimental conditions at laboratory facilities of the Academic Unit Carmen Pampa Campesina, a community of Carmen Pampa, Township Coroico. The aim of this study was to biologically control the "damping off", they found two genera that cause damping off in seedbed of coffee: Rhizoctonia sp. and Fusarium sp.To determine the percentage of growth and control in the culture medium, we used the method of counting quarters, where they gave the mycelial growth of antagonistic fungus Trichoderma sp., And the fungal pathogens Rhizoctonia sp. and Fusarium sp. Statistically there was a highly significant difference in the variable growth rate of Trichoderma sp. on pathogenic fungi Rhizoctonia sp. and Fusarium sp. at 3, 6 and 9 days that announces the time factor and treatments are interdependent. The control variable showed a highly significant difference in the time factor and treatment, but the interaction shows no significant difference this makes known factors that are independent, so the fungus Trichoderma sp. not depend on time in treatment, thus showing its inhibitory power to Rhizoctonia sp. and Fusarium sp .. This test gives references that there is antagonistic fungus control on the fungal pathogens Rhizoctonia sp. and Fusarium sp.

  16. CONSTRUCCIÓN DE UNA BIBLIOTECA GENÓMICA DE Coffea Arabica Var. COLOMBIA Y EVALUACIÓN CON UNA SECUENCIA HOMÓLOGA A UBIQUITINA

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    Elsa Leonor Álvarez Méndez 1, 2,

    2004-06-01

    Full Text Available Con el fin de buscar secuencias de interés en el genoma de Coffea arabica var. Colombia, se construyó una biblioteca genómica en el vector Lambda FIX II (Stratagene con un tamaño promedio de inserto de 15Kb y con un título de 1,33x106 ufp/ml que representa aproximadamente 3,7 veces el genoma haploide.La biblioteca fue evaluada utilizando como sonda un producto de PCR amplificado con la combinación de iniciadores InhF - R631 y que presenta alta homología con secuencias tipo ubiquitinas de Arabidopsis thaliana y Oriza sativa. Se identificaron dos clones recombinantes (cof-ubi1 y cof-ubi2 que hibridizaron con la sonda tipo ubiquitina. Los resultados obtenidos indican que la biblioteca genómica es adecuada para la identificación de secuencias de interés, mapeo genético y estudios sobre regulación de la expresión de genes.

  17. Cuantificación de minerales K, Ca, Mg y P en pulpa y pergamino de café (Coffea arabica L. var. Typica

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    Jorge Geovanny Figueroa Hurtado

    2010-12-01

    Full Text Available Se trabajó con muestras de café Coffea arabica L., variedad Typica de tres lugares en Ecuador: Palanda, Vilcabamba y El Pangui, seleccionados por su disponibilidad y condiciones geográficas. Se analizó potasio, calcio y magnesio por espectrofotometría de absorción atómica; y fósforo por espectrofotometría visible, utilizando en todos los casos la digestión ácida para la preparación de las muestras. Con el objetivo de mantener la trazabilidad de los residuos se realizó el beneficio húmedo del café. Los resultados obtenidos para la pulpa de café expresados en base seca fueron: K 3,1 ± 0,43 %; Ca 0,46 ± 0,06 %; Mg 0,14 ± 0,01 % y P 0,13 ± 0,01 %. Para pergamino de café: K 0,16 ± 0,02 %; Ca 0,14 ± 0,05 %; Mg 0,06 ± 0,01 % y P 0,02 ± 0,01 %. En adición, se determinaron los minerales en el grano de café. Con estos resultados podemos mencionar que la pulpa de café es una buena fuente de potasio (especialmente, magnesio y fósforo. El pergamino pudiera ser considerado como fuente de fósforo.

  18. Characterization of Fatty Acid, Amino Acid and Volatile Compound Compositions and Bioactive Components of Seven Coffee (Coffea robusta Cultivars Grown in Hainan Province, China

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    Wenjiang Dong

    2015-09-01

    Full Text Available Compositions of fatty acid, amino acids, and volatile compound were investigated in green coffee beans of seven cultivars of Coffea robusta grown in Hainan Province, China. The chlorogenic acids, trigonelline, caffeine, total lipid, and total protein contents as well as color parameters were measured. Chemometric techniques, principal component analysis (PCA, hierarchical cluster analysis (HCA, and analysis of one-way variance (ANOVA were performed on the complete data set to reveal chemical differences among all cultivars and identify markers characteristic of a particular botanical origin of the coffee. The major fatty acids of coffee were linoleic acid, palmitic acid, oleic acid, and arachic acid. Leucine (0.84 g/100 g DW, lysine (0.63 g/100 g DW, and arginine (0.61 g/100 g DW were the predominant essential amino acids (EAAs in the coffee samples. Seventy-nine volatile compounds were identified and semi-quantified by HS-SPME/GC-MS. PCA of the complete data matrix demonstrated that there were significant differences among all cultivars, HCA supported the results of PCA and achieved a satisfactory classification performance.

  19. Phytochemical Profile and in vitro Assessment of the Cytotoxicity of Green and Roasted Coffee Oils (Coffea arabica L. and their Polar Fractions

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    Ana Paula Lorenzen Voytena

    2018-03-01

    Full Text Available Green Coffea arabica L. seed oil (GCO has been used as an active cosmetic ingredient in many skin care products, due to its composition and balance of fatty acids. On the other hand, while roasted coffee oil (RCO is mainly used for imparting aroma in the food industry, there is no data available to suggest its safety in cell-based model systems. In this context, the present study aims to evaluate the chemical composition of GCO, RCO, and their correspondent polar fractions (PFs; and assess their cytotoxicity and antioxidant potential in vitro. RCO and RCO PF exhibited significantly higher amounts of phenolic compounds, when compared to both GCO and GCO PF. In the DPPH assay, after 5 min of incubation, RCO inhibited about 80% of radicals, while GCO only achieved half of this activity. Similar results were also obtained for their PFs. Upon exposure to GCO, no cytotoxic effects were observed, in fact, there were slight increments in cell proliferation. Nevertheless, cell exposure to RCO led to significant decreases in cell viability. Increases in the concentration of coffee oil PFs were associated with correspondent relevant increased cytotoxicity. Upon hydrogen peroxide-induced oxidative stress, neither GCO nor RCO treatment were effective in protecting cells.

  20. Influence of the in vitro environment on the germination of somatic embryos of Coffea arabica L. cv. 'Caturra rojo' and Clematis tangutica K.

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    Raúl Barbon

    2017-07-01

    Full Text Available The in vitro environment is a factor that in recent years has begun to investigate, because gases such as oxygen, carbon dioxide and ethylene play an important role in the morphogenesis of somatic embryos and their development in plants. The objective of this work was to determine the effect of the CO2 on the germination of coffee somatic embryos (Coffea arabica L. cv. 'Caturra rojo' and clematis (Clematis tangutica K.. Three gas mixtures composed of CO2 concentrations (2.5, 5.0 and 10.0% combined with 21% O2 and two controls (passive exchange and forced ventilation were used. A positive effect of CO2 on the germination of somatic embryos in the torpedo stage in coffee and clematis was obtained, because in the treatments with passive exchange, where there was CO2 accumulation, germination of the somatic embryos was superior to the treatments with Forced ventilation. With 2.5% and 5.0% CO2, the germination process is stimulated while with 10.0% CO2 there is an inhibition of germination with the appearance of malformations and hyperhydricity.   Keywords: gaseous atmosphere, carbon dioxide, somatic embryogenesis, secondary embryogenesis, hyperhydricity

  1. Effect of entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae

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    Michele Potrich

    2018-01-01

    Full Text Available This study aimed to evaluate the effect of commercially used entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae. Four bioassays were performed: 1 pulverized entomopathogens on A. mellifera; 2 entomopathogens sprayed on a smooth surface; 3 entomopathogens sprayed on soy leaves; and 4 entomopathogens mixed with candy paste (sugar syrup. Five treatments were prepared: sterile distilled water (control, distilled water sterilized with Tween® 80 (0.01%, and the commercial entomopathogens Metarhizium anisopliae E9 (1.0 × 109 conidia mL−1, Beauveria bassiana PL63 (1.0 × 108 conidia mL−1 and Bacillus thuringiensis var. kurstaki HD-1 (3.0 × 108 spores mL−1. Each treatment consisted of five repetitions, with 20 workers per repetition, which were stored in a plastic box and, later, in a biological oxygen demand (B.O.D. incubator (27 ± 2 °C, RH of 60% ± 10%, 12-h photophase. The mortality of the workers was evaluated from 1 h to 240 h, and the data were analyzed using Bayesian inference. The workers killed by the ingestion of candy paste contaminated with the pathogens (products were randomly separated and selected for the removal of the midgut. Each midgut was fixed in Bouin's solution and prepared for histology. B. bassiana was verified to reduce the survival of A. mellifera workers in all bioassays. Moreover, M. anisopliae reduced the survival of A. mellifera workers directly sprayed, on a smooth surface and mixed with candy. B. thuringiensis reduced A. mellifera survival on a smooth surface and mixed with candy paste. However, its effects were lower than that observed by B. bassiana. The treatments with the biological products did not induce morphometric alterations in the midgut of A. mellifera. Keywords: Bayesian statistics, Entomopathogenic fungi, Entomopathogenic bacteria, Honeybee, Selectivity

  2. A new species of Crinibracon Quicke (Hymenoptera: Braconidae) parasitic on pupae of Hasora chromus (Cramer) (Lepidoptera: Hesperiidae) from India.

    Science.gov (United States)

    Gupta, Ankita; Achterberg, Cornelis Van; Chitrala, Malathi

    2016-08-29

    A new species, Crinibracon chromusae Gupta & van Achterberg sp. n., parasitic on pupae of Hasora chromus (Cramer) (Hesperiidae) on Millettia (= Pongamia) pinnata (L.) Panigrahi (Fabaceae), is described from India and compared with C. sinicus (Yang, Chen & Liu, 2008) from China, the only other species known with a similar general appearance. For the first time biological information for the genus Crinibracon Quicke, 1988, is given. Three species of hyperparasitoids, Philolema braconidis (Ferrière) (Hymenoptera: Eurytomidae), Nesolynx javanica Ferrière (Hymenoptera: Eulophidae), and an Eupelmus sp. (Hymenoptera: Eupelmidae) emerged along with C. chromusae sp. n. from pupae of H. chromus. The generic placement of this new species along with interesting parasitoid biology is discussed.

  3. Primer registro de Tremex fuscicornis (Hymenoptera: Siricidae para la Argentina en una plantación de álamos en Buenos Aires First record of Tremex fuscicornis (Hymenoptera: Siricidae in Argentina infesting a poplar plantation in Buenos Aires

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    Lucas Landi

    2011-12-01

    Full Text Available La presencia de la "avispa taladradora de las latifoliadas", Tremex fuscicornis Fabricius (Hymenoptera: Siricidae, fue detectada en una plantación de álamos de la provincia de Buenos Aires, lo que constituye el primer registro de la especie para la Argentina. Se describen aspectos morfológicos y biológicos de la misma.The presence of the woodwasp Tremex fuscicornis Fabricius (Hymenoptera: Siricidae was detected in a poplar plantation in Buenos Aires. This is the first record of the species in Argentina. Morphological and biological aspects of the woodwasp are described.

  4. Diversidade de abelhas (Hymenoptera, Apidae) ao longo de um gradiente latitudinal na Mata Atlântica

    OpenAIRE

    Gonçalves,Rodrigo Barbosa; Brandão,Carlos Roberto Ferreira

    2008-01-01

    A Mata Atlântica é um dos ambientes mais ricos e ameaçados do mundo, o que deveria ter estimulado em muito o estudo e a conservação do Bioma, mas a fauna de Hymenoptera permanece ainda relativamente pouco conhecida. Em especial, a fauna de abelhas da floresta ombrófila densa é pouco estudada em comparação à fauna das áreas abertas brasileiras. O projeto temático "Biodiversidade de Hymenoptera e Isoptera: riqueza e diversidade ao longo de um gradiente latitudinal na Mata Atlântica - a floresta...

  5. Optimization of coffee (Coffea arabica transformation parameters using uidA and hpt genes: effect of osmotic pre-treatment, helium pressure and target distance

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    Andrés M Gatica

    2009-11-01

    Full Text Available The aim of this work was to optimize the biolistic delivery parameters that affect the DNA delivery and stable expression of marker genes into coffee tissues (Coffea arabica. L. cvs. Caturra and Catuaí. The effect of osmotic preculture length, osmotic concentration of medium, Helium pressure and target distance on transient expression of the uidA gene in coffee leaves and somatic embryos were tested. The highest transient uidA expression was obtained when Caturra (18.3±2.8 and Catuaí (6.8±2.0 leaves and Catuaí embryos (80.0±7.4 were cultured for 5h on Yasuda medium complemented with 0.5M Mannitol +0.5M Sorbitol. The combination of 1100psi and a target distance of 9cm resulted in the highest number of blue spots per Caturra leaf segment (23.6±3.9, whereas for the Catuaí variety the combination of 1100psi and a target distance of six (10.2±1.9 and nine (8.2±1.9 cm gave the highest number of blue spots per leaf segment. The optimized protocol was tested with pCAMBIA 1 301 (uidA gene and the hpt gene, pCAMBIA 1 305.2 (uidA version GUSPlus ™ and the hpt gene and pCAMBIA 1 301-BAR (uidA gene and the bar gene. The highest number of blue spots was obtained when Caturra (54.6±5.7 and Catuaí (28.9±4.3 leaves were bombarded with pCAMBIA 1 305.2. Selection of bombarded coffee tissues with 100mg/l hygromicyn caused the oxidation of tissues. Rev. Biol. Trop. 57 (Suppl. 1: 151-160. Epub 2009 November 30.La presente investigación tuvo como objetivo optimizar los parámetros que afectan la incorporación y expresión de genes marcadores mediante biobalística en segmentos de hoja y embriones somáticos de café (Coffea arabica. L. cvs. Caturra y Catuaí. La mayor expresión transitoria del gen uidA en segmentos de hoja de Caturra (18.3±2.8 y Catuaí (6.8±2.0 y embriones somáticos de Catuaí (80.0±7.4 se obtuvo al cultivar los explantes por cinco horas previo al bombardeo en el medio Yasuda complementado con 0.5M mannitol+0.5M sorbitol

  6. Direct somatic embryogenesis in coffea arabica L. CVS. caturra and catuaí: effect of triacontanol, light condition, and medium consistency

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    Andrés M. Gatica

    2008-01-01

    Full Text Available Se investigó la influencia de la concentración de triacontanol (TRIA y su interacción con el ácido indolacético (AIA en la inducción de la embriogénesis somática directa en Coffea arabica L. cvs. Caturra y Catuaí. Adicionalmente, se evaluó el efecto de la condición de la luz y de la consistencia del medio de cultivo (semisólido vs. líquido. Se determinó que la mayor cantidad de embriones somáticos por explante fue de 3,9±0,5 en Caturra y 3,6±0,5 en Catuaí, en el medio de cultivo Murashige y Skoog (1962 al 50%, complementado con BAP (1,1 µM, IAA (2,85 µM y TRIA (4,55 µM. Para la consistencia del medio, la mayor cantidad de embriones somáticos en Caturra (3,2±0,2 y Catuaí (6,0±0,4 se obtuvo con el medio semisólido de Yasuda et al. (1985. En Catuaí, la mayor cantidad de embrioides se obtuvo con 16 h luz (7,6±1,0 y oscuridad (6,2±0,6 y para Caturra, con oscuridad (4,2±0,4 y 16 h luz (3,8±0,5. No se observó la formación de embriones somáticos en los explantes de Caturra y Catuaí después de 12 semanas de cultivo, en el medio líquido descrito por Yasuda et al. (1985 ni bajo ninguna de las condiciones de luz.

  7. Method Development and Validation for the Determination of Caffeine: An Alkaloid from Coffea arabica by High-performance Liquid Chromatography Method.

    Science.gov (United States)

    Naveen, P; Lingaraju, H B; Deepak, M; Medhini, B; Prasad, K Shyam

    2018-01-01

    The present study was investigated to develop and validate a reversed phase high performance liquid chromatography method for the determination of caffeine from bean material of Coffee arabica. The separation was achieved on a reversed-phase C18 column using a mobile phase composed of water: methanol (50:50) at a flow rate of 1.0 mlmin-1. The detection was carried out on a UV detector at 272 nm. The developed method was validated according to the requirements for International Conference on Harmonisation (ICH) guidelines, which includes specificity, linearity, precision, accuracy, limit of detection and limit of quantitation. The developed method validates good linearity with excellent correlation coefficient (R2 > 0.999). In repeatability and intermediate precision, the percentage relative standard deviation (% RSD) of peak area was less than 1% shows high precision of the method. The recovery rate for caffeine was within 98.78% - 101.28% indicates high accuracy of the method. The low limit of detection and limit of quantitation of caffeine enable the detection and quantitation of caffeine from C. arabica at low concentrations. The developed HPLC method is a simple, rapid, precisely, accurately and widely accepted and it is recommended for efficient assays in routine work. A simple, accurate, and sensitive high-performance liquid chromatography (HPLC) method for caffeine from Coffea arabica has been developed and validated. The developed HPLC method was validated for linearity, specificity, precision, recovery, limits of detection, and limits of quantification by the International Conference on Harmonization guidelines. The results revealed that the proposed method is highly reliable. This method could be successfully applied for routine quality work analysis. Abbreviation Used: C. arabica : Coffee arabica, ICH: International Conference on Harmonisation, % RSD: Percentage Relative Standard Deviation, R2: Correlation Coefficient, ppm: Parts per million, LOD: Limits

  8. Storage of soil organic carbon in coffee (Coffea arabica L. production systems in the municipality of Líbano, Tolima, Colombia

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    Hernán Jair Andrade Castañeda

    2013-07-01

    Full Text Available The increase in greenhouse gas emissions from anthropogenic sources has resulted in climate change, which affects all living beings. Coffee (Coffea arabica L. plantations, in monoculture or together with timber species such as salmwood (Cordia alliodora, mitigate climate change due to fixation of atmospheric CO2 that is deposited in biomass and soils. This study was carried out in the municipality of Líbano, Tolima, Colombia with the objective of defining which of the following coffee production systems store more soil organic carbon (SOC: 1 monoculture, 2 agroforestry systems (AFS with salmwood, and 3 AFS with plantain. Farms with those systems that are the most dominant in the study zone were selected. From each system, five repetitions were identified to be analyzed with a completely randomized design. In each plot or repetition, five samples for bulk density (BD using the cylinder method and a composite sample for concentration of SOC were taken and analyzed using the Walkley and Black approach. The systems of production did not significantly (p > 0.05 affect either the BD or the concentration of SOC. However, AFS with plantain tended to have less BD than monoculture and AFS with salmwood (0.83 ± 0.03 vs 0.88 ± 0.03 vs 0.92 ± 0.04 g·cm-3, respectively. These systems of production stored between 50 and 54 t·ha-1 of SOC in the top 30 cm, which indicates their capacity for climate change mitigation.

  9. Embryogenic potential and the expression of arabinogalactanproteins (AGPs in embryogenic cell aggregates of Coffea arabica cv. Caturra rojo under influence of CO2

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    Raúl Barbón

    2002-07-01

    Full Text Available The establishment and optimization of regeneration systems via somatic embryogenesis has centered traditionally on the study of the culture medium components, with little attention to other factors of the in vitro environment like the composition of the gaseous atmosphere. This work was realized with the aim to determine the embryogenic potential and the expression of arabinogalactanproteins (AGPs in embryogenic cell aggregates of Coffea arabica cv. Caturra Rojo under influence of carbon dioxide (CO2. Concentrations of 2.5% and 5.0% of CO2 stimulated a greater formation of somatic embryos (307 ES/50mgMF and 277 ES/50mgMF respectively in semisolid culture medium with better results to the controls with passive interchange and forced ventilation (195 ES/50mgMF and 107 ES/50mgMF whereas a concentration of 10.0% of CO2 inhibited the process of somatic embryogenesis (95 ES/50mgMF. In cell suspensions the formation of somatic embryos was greater with a concentration of 2.5% of CO2 (130x103 ES.l-1 in comparison with 5.0% and 10.0% of CO2 (116x103 ESl-1 and 15x103 ES.l-1 and the controls with passive interchange and forced ventilation. With a concentration of 2.5% CO2 there was a greater synthesis of Arabinogalactanproteins (AGPs at the cellular level (0.068 mg.gMF-1 and the excretion in the culture medium (2.10 mg.l-1, which coincided with the highest values of production of somatic embryos. Key Words: embryogenic cell suspensions, in vitro environment, molecular markers, somatic embryo, somatic embryogenesis

  10. Producción de café (Coffea Arabica L. en respuesta al manejo específico por sitio de la fertilidad del suelo

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    Luz Adriana Lince Salazar

    2016-12-01

    Full Text Available El estudio de la variabilidad espacial es el principal insumo para el manejo específico por sitio MES, con el fin de optimizar los recursos, al reducir los costos de producción de los cultivos y el impacto negativo sobre el ambiente. Con el objetivo de determinar el efecto del MES en la producción del café, durante los años 2011 y 2012 se realizó un experimento del manejo de la fertilidad del suelo en café (Coffea arabica L., Variedad Castillo® en lotes con áreas diferentes (1,0, 2,0 y 3,5 ha, ubicados en tres fincas del departamento del Quindío, Colombia. Se evaluó el efecto de tres tratamientos en la producción de café cereza: i testigo sin fertilización; ii manejo de acuerdo a los resultados de los análisis de suelo, logrados mediante un muestreo convencional en zigzag y, iii manejo por sitio específico conforme lo indicaron las áreas homogéneas, obtenidas mediante mapas de variabilidad espacial. En ninguno de los lotes hubo efecto de los tratamientos, respuesta que se relacionó con uno o más de los siguientes factores: tiempo relativamente corto de evaluación, fertilidad del suelo, reservas de los nutrientes en las plantas, y el número reducido de repeticiones (tres por tratamiento.

  11. Producción de café (Coffea arabica L. en respuesta al manejo específico de la fertilidad del suelo

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    Luz Adriana Lince Salazar

    2015-12-01

    Full Text Available El estudio de la variabilidad espacial es el principal insumo para el manejo específico por sitio-MES, con fines de optimizar los recursos, al reducir los costos de producción de los cultivos y el impacto negativo sobre el ambiente. Con el objetivo de determinar el efecto del MES en la producción del café, durante los años 2011 y 2012 se realizó un experimento del manejo de la fertilidad del suelo en café (Coffea arabica L., Variedad Castillo® en lotes con áreas diferentes (1,0, 2,0 y 3,5 ha, ubicados en tres fincas del departamento del Quindío, Colombia. Se evaluó el efecto de tres tratamientos en la producción de café cereza: i testigo sin fertilización; ii manejo de acuerdo a los resultados de los análisis de suelo, logrados mediante un muestreo convencional en zigzag y, iii manejo por sitio específico conforme lo indicaron las áreas homogéneas, obtenidas mediante mapas de variabilidad espacial. En ninguno de los lotes hubo efecto de los tratamientos, respuesta que se relacionó con uno o más de los siguientes factores: tiempo relativamente corto de evaluación, fertilidad del suelo, reservas de los nutrientes en las plantas, y el número reducido de repeticiones (tres por tratamiento.

  12. Arbuscular mycorrhiza alters metal uptake and the physiological response of Coffea arabica seedlings to increasing Zn and Cu concentrations in soil.

    Science.gov (United States)

    Andrade, S A L; Silveira, A P D; Mazzafera, P

    2010-10-15

    Studies on mycorrhizal symbiosis effects on metal accumulation and plant tolerance are not common in perennial crops under metal stress. The objective of this study was to evaluate the influence of mycorrhization on coffee seedlings under Cu and Zn stress. Copper (Cu) and zinc (Zn) uptake and some biochemical and physiological traits were studied in thirty-week old Coffea arabica seedlings, in response to the inoculation with arbuscular mycorrhizal fungi (AMF) and to increasing concentrations of Cu or Zn in soil. The experiments were conducted under greenhouse conditions in a 2×4 factorial design (inoculation or not with AMF and 0, 50, 150 and 450mgkg(-1) Cu or 0, 100, 300 and 900mgkg(-1) Zn). Non-mycorrhizal plants maintained a hampered and slow growth even in a soil with appropriate phosphorus (P) levels for this crop. As metal levels increased in soil, a greater proportion of the total absorbed metals were retained by roots. Foliar Cu concentrations increased only in non-mycorrhizal plants, reaching a maximum concentration of 30mgkg(-1) at the highest Cu in soil. Mycorrhization prevented the accumulation of Cu in leaves, and mycorrhizal plants showed higher Cu contents in stems, which indicated a differential Cu distribution in AMF-associated or non-associated plants. Zn distribution and concentrations in different plant organs followed a similar pattern independently of mycorrhization. In mycorrhizal plants, only the highest metal concentrations caused a reduction in biomass, leading to significant changes in some biochemical indicators, such as malondialdehyde, proline and amino acid contents in leaves and also in foliar free amino acid composition. Marked differences in these physiological traits were also found due to mycorrhization. In conclusion, AMF protected coffee seedlings against metal toxicity. Copyright © 2010 Elsevier B.V. All rights reserved.

  13. Crecimiento de café (Coffea arabica L. durante la etapa de almácigo en respuesta a la salinidad generada por fertilizantes

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    Siavosh Sadeghian Khalajabadi

    2014-12-01

    Full Text Available La salinidad del suelo puede afectar negativamente el crecimiento de las plantas, e incluso causar su muerte. Esta investigación tuvo como propósito, determinar el efecto de la salinidad generada por fertilizantes en el crecimiento de café Coffea arabica L. durante la etapa de almácigo. Se recolectaron muestras de suelo en dos Estaciones Experimentales (EE de Cenicafé (Naranjal en Caldas y Timbío en Cauca y una finca cafetera (FC en Jamundí (Valle del Cauca. Bajo el diseño completamente aleatorio se evaluó para cada suelo la respuesta de café variedad Caturra a 27 tratamientos (factorial 33, resultantes de tres dosis de calcio-Ca, de magnesio-Mg y de potasio-K (0, 1 y 3 g dm-3 de suelo, empleando como fuentes sulfatos. La conductividad eléctrica (C.E., como indicador de la salinidad, se incrementó en conformidad a las dosis, con valores más altos para el Mg, seguido por K y Ca, resultado que se relacionó principalmente con el índice de salinidad de los fertilizantes y su solubilidad. Las diferencias de la C.E. entre las localidades (EE Naranjal>EE Timbío>FC Jamundí se atribuyeron a la capacidad de éstos para retener la humedad. Conforme al aumento de la salinidad disminuyó el peso de las plantas, hasta causar síntomas de toxicidad y posterior muerte. El nivel crítico de la C.E., calculado para el 90% del crecimiento relativo, correspondió en promedio a 1,1 dS m-1.

  14. The first record of the genus Tanaostigma (Hymenoptera: Tanaostigmatidae) in the Old World, with the description of a new species from India.

    Science.gov (United States)

    Gupta, Ankita; Joshi, Sunil

    2016-11-15

    Tanaostigma Howard (Hymenoptera: Tanaostigmatidae) is recorded for the first time in the fauna of the Old World, with T. indica Gupta sp. n.  described and illustrated from southern India, reared from Millettia (=Pongamia) pinnata (Fabaceae).

  15. Effects of feeding frequency and sugar concentration on behavior and longevity of the adult aphid parasitoid: Aphidius ervi (Haliday) (Hymenoptera: Braconidae)

    NARCIS (Netherlands)

    Azzouz, H.; Giordanengo, P.; Wäckers, F.L.; Kaiser, L.

    2004-01-01

    Aphidius ervi (Haliday) (Hymenoptera: Braconidae) is a solitary aphid endoparasitoid. Adults feed on honeydew and possibly on other sugar sources such as nectar. Sugar sources can vary qualitatively and quantitatively according to biotic factors and environmental conditions. Experiments were

  16. Oviposition behavior and survival of Tamarixia radiata (Hymenoptera: Eulophidae), an ectoparasitoid of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), on hosts exposed to an entomopathogenic fungus

    Science.gov (United States)

    Antagonistic interactions between the nymphal parasitoid, Tamarixia radiata Waterston (Hymenoptera: Eulophidae), and the ARSEF 3581 isolate of the entomopathogenic fungus, Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae) could disrupt biological control of the Asian citrus psyllid, Diaphorina ...

  17. Notes on Apidae and Vespidae (Hymenoptera) Species Collected by Bait Traps in OrganicVineyard and Orchards of Kemalpaşa (İzmir), Western Turkey

    OpenAIRE

    ÜZÜM, Ahu; TANYERİ, Rukiye; GÜLPERÇİN, Nilay; TEZCAN, Serdar; YILDIRIM, Erol

    2010-01-01

    Hymenoptera species collected by bait traps during the months of June-October in organic vineyard and orchards in Kemalpaşa district, (İzmir) of Western Turkey were evaluated in this study. As a result, six species belonging 2007 to two families of Hymenoptera were determined. Those were Apis mellifera Linnaeus, 1758, Vespula germanica (Fabricius, 1793), Vespa crabro Linnaeus, 1758, Vespa orientalis Linnaeus, 1771, Polistes dominulus (Christ, 1791) and Polistes gallicus (Linnaeus, 1767). Amon...

  18. Improved sensitivity to venom specific-immunoglobulin E by spiking with the allergen component in Japanese patients suspected of Hymenoptera venom allergy

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    Naruo Yoshida

    2015-07-01

    Conclusions: The measurement of sIgE following spiking of rVes v 5 and rPol d 5 by conventional testing in Japanese subjects with sIgE against hornet and paper wasp venom, respectively, improved the sensitivity for detecting Hymenoptera venom allergy. Improvement testing for measuring sIgE levels against hornet and paper wasp venom has potential for serologically elucidating Hymenoptera allergy in Japan.

  19. A new species of genus Chorebus Haliday (Hymenoptera, Alysiinae parasitising Hexomyza caraganae Gu (Diptera, Agromyzidae from NW China

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    Tao Li

    2017-03-01

    Full Text Available Chorebus (Stiphrocera hexomyzae sp. n. (Hymenoptera, Braconidae, Alysiinae, Dacnusini is described and illustrated. It was reared from twig galls of Hexomyza caraganae Gu (Diptera, Agromyzidae on Caragana korshinskii Kom. f. (Fabaceae in Ningxia and Inner Mongolia (NW China. A partial key to related or similar Chorebus species is provided.

  20. Preliminary taxonomic study of the genus Praon (Hymenoptera:Braconidae:Aphidiinae) and its host associations in Iran

    Czech Academy of Sciences Publication Activity Database

    Rakhshani, E.; Talebi, A. A.; Manzari, S.; Tomanovic, Ž.; Starý, Petr; Rezwani, A.

    2007-01-01

    Roč. 26, č. 2 (2007), s. 19-34 ISSN 0259-9996 R&D Projects: GA AV ČR IBS5007102 Institutional research plan: CEZ:AV0Z50070508 Keywords : genus Praon * Hymenoptera * Iran Subject RIV: EH - Ecology, Behaviour

  1. Temperature dependent functional response of Diaeretiella rapae (Hymenoptera: Aphidiidae) to the cabbage aphid, Brevicoryne brassicae (Hemiptera: Aphididae)

    DEFF Research Database (Denmark)

    Moayeri, Hamid R. S.; Madadi, Hossein; Pouraskari, Hossein

    2013-01-01

    Diaeretiella rapae MacIntosh (Hymenoptera: Aphidiidae) is one of the most common and successful parasitoids of the cabbage aphid. The functional response of D. rapae towards cabbage aphids was examined in laboratory studies at three constant temperatures, 17°C, 25°C and 30°C. D. rapae exhibited a...

  2. Biological parameters and thermal requirements of the parasitoid Praon volucre (Hymenoptera: Braconidae) with Macrosiphum euphorbiae (Hemiptera: Aphididae) as host

    NARCIS (Netherlands)

    Conti, De B.F.; Bueno, V.H.P.; Sampaio, M.V.; Lenteren, van J.C.

    2011-01-01

    The effect of temperature on the biology of Praon volucre (Haliday, 1833) (Hymenoptera: Braconidae) in Macrosiphum euphorbiae (Thomas, 1878) (Hemiptera: Aphididae) hosts was studied and the thermal requirements of the parasitoid were determined. Experiments were carried out at 16, 19, 22, 25, and 28

  3. A new species of Tamarixia Mercet (Hymenoptera, Eulophidae, parasitoid of Trioza aguacate Hollis & Martin (Hemiptera, Triozidae in Mexico

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    Zoya Yefremova

    2014-01-01

    Full Text Available Tamarixia aguacatensis Yefremova, sp. n. (Hymenoptera: Eulophidae: Tetrastichinae is described from Mexico as a parasitoid of the avocado psyllid, Trioza aguacate Hollis & Martin (Hemiptera: Triozidae. Trioza aguacate is a serious pest of avocado, Persea americana Miller. A key to the species of Tamarixia Mercet in Mexico is given.

  4. Sex determination in the haplodiploid wasp Nasonia vitripennis (Hymenoptera Chalcidoidea) : A critical consideration of models and evidence

    NARCIS (Netherlands)

    Beukeboom, Leo W.; Kamping, Albert; van de Zande, Louis

    Sex determining mechanisms are highly diverse. Like all Hymenoptera, the parasitic wasp Nasonia vitripennis reproduces by haplodiploidy: males are haploid and females are diploid. Sex in Nasonia is not determined by complementary alleles at sex loci. Evidence for several alternative models is

  5. Record of the genus Arrhenophagoidea Girault (Hymenoptera: Chalcidoidea: Encyrtidae from India, description of a new species from the Andaman Islands

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    M. Hayat

    2014-05-01

    Full Text Available The genus Arrhenophagoidea Girault (Hymenoptera: Encyrtidae is recorded for the first time from India and the Oriental region, and a new species, A. andamanica sp. Nov. is described from material collected in the Andaman and Nicobar Islands, India. As the genus is newly recorded from the Oriental region, a brief diagnosis is also given.

  6. A new species of the Camponotus aureopilus VIEHMEYER, 1914 species-group (Hymenoptera: Formicidae) from Papua New Guinea

    Czech Academy of Sciences Publication Activity Database

    Shattuck, S.; Janda, Milan

    2009-01-01

    Roč. 12, - (2009), s. 251-253 ISSN 1994-4136 R&D Projects: GA MŠk LC06073; GA AV ČR KJB612230701 Grant - others:U.S. National Science Foundation(US) DEB-02-11591 Institutional research plan: CEZ:AV0Z50070508 Keywords : taxonomic description * Hymenoptera * Formicidae Subject RIV: EG - Zoology

  7. A new species of Megischus Brullé (Hymenoptera, Stephanidae from China, with a key to the Chinese species

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    Hong Chun-dan

    2010-11-01

    Full Text Available A new species of Megischus Brullé from China, M. aplicatus sp. n., is described and illustrated. A key to the Chinese species of Megischus is added. The holotype is deposited in the Parasitic Hymenoptera Collection of Zhejiang University, Hangzhou.

  8. Two new species of Oobius (Hymenoptera: Encyrtidae) and their phylogenetic relationship with other congeners from northeastern Asia

    Science.gov (United States)

    Yan-Xia Yao; Jian J. Duan; Jason L. Mottern; Xiao-Yi Wang; Zhong-Qi Yang; Leah S. Bauer; Michael W. Gates

    2018-01-01

    Two new species of egg parasitoids, Oobius saimaensis Yao and Mottern new species and Oobius fleischeri Yao and Duan new species (Hymenoptera: Encyrtidae), are described from eggs of Agrilus fleischeri Obenberger, 1925 (Coleoptera: Buprestidae). Agrilus fleischeri is a phloemfeeding woodborer of poplar (...

  9. The parasites of cereal stem borers (Lepidoptera: Cossidae, Crambidae, Noctuidae, Pyralidae) in Africa, belonging to the family Braconidae (Hymenoptera: Ichneumonoidea)

    NARCIS (Netherlands)

    Achterberg, van C.; Polaszek, A.

    1996-01-01

    A review is given of the parasites (parasitoids) of the African cereal stem borers (including introduced species) belonging to the family Braconidae (Hymenoptera); 38 species belonging to 19 genera are keyed and treated. Three new species are described: Macrocentrus sesamivorus spec. nov. from

  10. New species of Megastylus (Hymenoptera: Ichneumonidae: Orthocentrinae) reared from larvae of Keroplatidae fungus gnats (Diptera) in a Dutch orchid greenhouse

    NARCIS (Netherlands)

    Humala, Andrei E.; Kruidhof, Marjolein; Woelke, Joop

    2017-01-01

    A new parasitoid wasp species belonging to the genus Megastylus (Hymenoptera: Ichneumonidae: Orthocentrinae) found in an orchid nursery in The Netherlands is described and illustrated: Megastylus woelkei sp. nov. It was reared from parasitized larvae of fungus gnats (Diptera: Keroplatidae). The

  11. An epidemiological survey of hymenoptera venom allergy in the Spanish paediatric population.

    Science.gov (United States)

    Martínez-Cañavate, A; Tabar, A I; Eseverri, J L; Martín, F; Pedemonte-Marco, C

    2010-01-01

    Hypersensitivity reactions to hymenoptera venom are infrequent in paediatric patients. A study was made to determine the incidence of this pathology in children, based on an epidemiological survey targeted to all members of the SEICAP (Sociedad Española de Inmunología Clínica y Alergia Pediátrica/Spanish Society of Paediatric Clinical Immunology and Allergy), and designed to collect the data on patients under 17 years of age diagnosed with hymenoptera venom allergy. The data corresponding to 175 patients (135 males) were collected. The mean age was 9.9 ± 3.6 years. Seventeen percent (32 patients) were the offspring of beekeepers, and 68.9% had experienced previous stings. The causal insect was Apis melifera, implicated in 55 cases, followed by Polistes dominulus (33 cases). In 151 patients (83.9%) the condition consisted of a local reaction. The most frequent systemic response was urticaria and angio-oedema. Fourteen patients suffered anaphylactic shock. The diagnosis was based on skin test (intradermal and prick) and/or specific IgE testing. Three treatment categories were established: (a) prevention and educational measures; (b) symptomatic treatment with oral antihistamines as well as self-injectable adrenalin; and (c) immunotherapy. In this context, 135 patients underwent immunotherapy with a mean duration of 3.5 ± 1.7 years (range 2-5 years) - with excellent tolerance. The starting regimen was predominantly conventional (92 patients). The results of this survey show hypersensitivity reactions to hymenoptera venom to be infrequent in paediatrics, though with a strong impact upon patient quality of life. Copyright © 2009 SEICAP. Published by Elsevier Espana. All rights reserved.

  12. Hymenoptera of Afghanistan and the central command area of operations: assessing the threat to deployed U.S. service members with insect venom hypersensitivity.

    Science.gov (United States)

    Turbyville, Joseph C; Dunford, James C; Nelson, Michael R

    2013-01-01

    Insect venom hypersensitivity can pose a threat to personnel deployed to a combat zone but the exposure risk in Afghanistan is currently unknown. This study was designed to assess the threat of Hymenoptera stings and associated allergic reactions in Afghanistan. Hymenoptera species were collected during a deployment to southern Afghanistan from June 2010 through January 2011. The literature was also reviewed to determine species of medically important Hymenoptera recorded in the region. The U.S. Army theater electronic medical data system was mined for ICD-9 codes associated with insect stings to determine the number of theater medical clinic encounters addressing insect sting reactions. Three species of flying hymenoptera were commonly encountered during the study period: Vespa orientalis L., Polistes wattii Cameron, and Vespula germanica (F.). A literature review also confirms the presence of honeybees (Apidae), numerous velvet ant (Mutillidae) species, and various ant (Formicidae) species all capable of stinging. No evidence was identified to suggest that fire ants (Solenopsis ssp.) are a threat in the region. Based on electronic medical records from the U.S. Central Command area of operations over a 2-year period, roughly 1 in 500 clinic visits involved a patient with a diagnosis of insect bite or sting. Cross-reactive members of all five flying Hymenoptera species commonly assessed for in Hymenoptera allergy evaluations are present in Afghanistan. The review of in-theater medical records confirms that insect stings pose an environmental threat to deployed service members.

  13. PERBANDINGAN KEANEKARAGAMAN HYMENOPTERA PARASITOID PADA AGROEKOSISTEM KEDELAI DENGAN APLIKASI DAN TANPA APLIKASI INSEKTISIDA

    Directory of Open Access Journals (Sweden)

    Hendrival Hendrival

    2017-04-01

    Full Text Available Abstrak Sistem pengelolaan tanaman kedelai dengan penggunaan insektisida sintetik yang intensif akan menurunkan keanekaragaman jenis Hymenoptera parasitoid. Penelitian bertujuan untuk memban-dingkan keanekaragaman Hymenoptera parasitoid pada agroekosistem kedelai dengan dan tanpa aplikasi insektisida sintetik. Pengumpulan data serangga menggunakan perangkap dari jaring serangga dan nampan kuning. Hasil penelitian menunjukkan bahwa indeks keanekaragaman jenis pada fase pertumbuhan vegetatif dan generatif kedelai dengan aplikasi insektisida lebih rendah dibandingkan dengan yang tanpa aplikasi insektisida, yang keduanya tergolong sedang. Indeks kemerataan jenis pada fase pertumbuhan vegetatif dan generatif dari kedua agroekosistem kedelai tergolong tinggi. Indeks kekayaan jenis pada fase vegetatif dari agroekosistem kedelai dengan aplikasi insektisida tergolong rendah (0<2,3955≤2,5, sedangkan pada fase generatif tergolong sedang (0<3,6118≤4. Indeks kekayaan jenis pada fase vegetatif (0<2,6229≤4 dan generatif (0<3,8287≤4 dari agroekosistem kedelai tanpa aplikasi insektisida tergolong sedang. Komunitas Hymenoptera parasitoid pada agroekosistem kedelai tanpa aplikasi insektisida memiliki kemiripan lebih rendah daripada yang dengan aplikasi insektisida. Aplikasi insektisida mempengaruhi indeks kekayaan jenis pada fase generatif dan kemiripan komunitasnya, yaitu nilainya lebih rendah daripada yang tanpa insektisida. Abstract The management system of soybean agroecosystem with an intensive use of synthetic insecticides will reduce the diversity of parasitoid Hymenoptera species. The study aimed to compare the diversity of the parasitoids in soybean agroecosystem with and without insecticide application. The collection of the parasitoid used insect net and yellow tray. The results showed that the diversity index of the parasitoids during vegetative and generative growth of the soybean with the insecticide application was lower than the one without

  14. Evolution of Cuticular Hydrocarbons in the Hymenoptera: a Meta-Analysis.

    Science.gov (United States)

    Kather, Ricarda; Martin, Stephen J

    2015-10-01

    Chemical communication is the oldest form of communication, spreading across all forms of life. In insects, cuticular hydrocarbons (CHC) function as chemical cues for the recognition of mates, species, and nest-mates in social insects. Although much is known about the function of individual hydrocarbons and their biosynthesis, a phylogenetic overview is lacking. Here, we review the CHC profiles of 241 species of Hymenoptera, one of the largest and most important insect orders, which includes the Symphyta (sawflies), the polyphyletic Parasitica (parasitoid wasps), and the Aculeata (wasps, bees, and ants). We investigated whether these taxonomic groups differed in the presence and absence of CHC classes and whether the sociality of a species (solitarily vs. social) had an effect on CHC profile complexity. We found that the main CHC classes (i.e., n-alkanes, alkenes, and methylalkanes) were all present early in the evolutionary history of the Hymenoptera, as evidenced by their presence in ancient Symphyta and primitive Parasitica wasps. Throughout all groups within the Hymenoptera, the more complex a CHC the fewer species that produce it, which may reflect the Occam's razor principle that insects' only biosynthesize the most simple compound that fulfil its needs. Surprisingly, there was no difference in the complexity of CHC profiles between social and solitary species, with some of the most complex CHC profiles belonging to the Parasitica. This profile complexity has been maintained in the ants, but some specialization in biosynthetic pathways has led to a simplification of profiles in the aculeate wasps and bees. The absence of CHC classes in some taxa or species may be due to gene silencing or down-regulation rather than gene loss, as demonstrated by sister species having highly divergent CHC profiles, and cannot be predicted by their phylogenetic history. The presence of highly complex CHC profiles prior to the vast radiation of the social Hymenoptera indicates a

  15. Taxonomic and faunistic study of Aulacidae (Hymenoptera, Evanioidea from Iran, with illustrated key to species

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    Mostafa Ghafouri Moghaddam

    2018-02-01

    Full Text Available Aulacidae are parasitoids of wood-boring larvae of Hymenoptera and Coleoptera, known in all zoogeographic regions of the World, except Antarctic. Two aulacids, Pristaulacus compressus (Spinola, 1808 and the rare Pristaulacus mourguesi Maneval, 1935, have been recently collected from Iran, the latter being a new record. Based on available data, the Iranian aulacid fauna includes five species within a single genus, Pristaulacus Kieffer 1900. A brief taxonomic treatment, as well as morphometric data and an illustrated key to species, are provided.

  16. Contribution to Taxonomy and Distribution of the Genus Elaphropoda (Hymenoptera: Apidae: Apinae in Vietnam

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    Nguyen, Minh Phuong

    2016-04-01

    Full Text Available Taxonomic notes on the genus Elaphropoda Lieftinck, 1966 (Hymenoptera: Apidae from Vietnam are presented. Two species of the genus are reported: Elaphropoda percarinata (Cockerell, 1930 was first recorded from Vietnam based on specimens collected from Ha Tinh province in 1998, and is reconfirmed with a specimens collected from Bac Kan province in the Northeastern part of the country in this study, and Elaphropoda khasiana (Schulz, 1906 is recorded from Vietnam for the first time. Redescriptions of the male of E. percarinata and the female of E. khasiana are given with illustrations.

  17. Primer registro del género Mellinus (Hymenoptera: Sphecidae en Colombia

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    Fernández C. Fernando

    1999-06-01

    Full Text Available Uno de los taxones menos conocidos de Sphecidae (Hymenoptera: Apoidea es Mellinus Fabricius, género que en el pasado llegó a tener categoría de subfamilia (Bohart & Menke 1976, pero que ahora comprende una tribu, Mellinini, dentro de la subfamilia Nyssoninae (Menke & Fernández 1996. Al parecer,  las hembras de este género capturan moscas del estiércol cerca a excrementos de mamíferos en el campo, y hacen sus nidos en el suelo (Evans 1989.

  18. Utility of laboratory testing for the diagnosis of Hymenoptera venom allergy.

    Science.gov (United States)

    Vachová, Martina; Panzner, Petr; Malkusová, Ivana; Hanzlíková, Jana; Vlas, Tomáš

    2016-05-01

    A diagnosis of Hymenoptera venom allergy is based on clinical history and the results of skin tests and/or laboratory methods. To analyze the utility of available laboratory tests in diagnosing Hymenoptera venom allergy. Ninety-five patients with Hymenoptera venom allergy with a history of bee (35) or wasp (60) anaphylactic sting reaction and positive skin test with bee or wasp venom were included in this analysis. Specific immunoglobulin E (to bee venom extract, wasp venom extract, available recombinant molecules, and a basophil activation test with venom extracts were assessed in all the patients. Test sensitivity and specificity were calculated by using standard threshold values; then, receiver operating characteristic curve analysis was performed to compute optimal threshold values. Also, statistical analysis of the utility of different combinations of laboratory tests was performed. The optimal threshold values were revealed to be the following: 1.0 kIU/L for bee venom extract (sensitivity, 97.14%; specificity, 100%), 0.35 kIU/L for rApi m 1 (sensitivity, 68.57%; specificity, 100%), 1.22 kIU/L for wasp venom extract (sensitivity, 88.33%; specificity, 95.45%), 0.7 kIU/L for rVes v 5 (sensitivity, 86.67%; specificity, 95.45%), 1.0 kIU/L for rVes v 1 (sensitivity, 56.67%; specificity, 95.45%), 6.5% for basophil activation test with bee venom extract (sensitivity, 80%; specificity, 95.45%), and 4.5% for basophil activation test with wasp venom extract (sensitivity, 91.53%; specificity, 95.45%). The best test combinations were found to be the following: bee venom extract plus rApi m 1 (sensitivity, 97.14%; specificity, 95.45%) in bee and either wasp venom extract plus rVes v 5, or rVes v 5 plus rVes v 1 (both sensitivity, 98.33%; specificity, 95.45%) in patients with wasp venom allergy. Our analysis confirmed that currently used laboratory tests represent effective tools in diagnosing Hymenoptera venom allergy. Moreover, our probabilistic approach offered another

  19. Pollinator diversity (Hymenoptera and Diptera in semi-natural habitats in Serbia during summer

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    Mudri-Stojnić Sonja

    2012-01-01

    Full Text Available The aim of this study was to assess species diversity and population abundance of the two main orders of pollinating insects, Hymenoptera and Diptera. The survey was conducted in 16 grassland fragments within agro-ecosystems in Vojvodina, as well as in surrounding fields with mass-flowering crops. Pollinators were identified and the Shannon-Wiener Diversity Index was used to measure their diversity. Five families, 7 subfamilies, 26 genera and 63 species of insects were recorded. All four big pollinator groups investigated were recorded; hoverflies were the most abundant with 32% of the total number of individuals, followed by wild bees - 29%, honeybees - 23% and bumblebees with 16%.

  20. Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae

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    Danúncia Urban

    2011-06-01

    Full Text Available Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae. Melostelis gen. nov. é proposto para um novo Anthidiini cleptoparasita. São descritas e ilustradas duas espécies novas: Melostelis amazonensis sp. nov. de Manaus, Amazonas e Larocanthidium chacoense sp. nov. de Porto Murtinho, Mato Grosso do Sul. São dados a conhecer os machos de Epanthidium bolivianum Urban, 1995 e Epanthidium araranguense Urban, 2006 e, registrados pela primeira vez no Brasil, na sub-região do chaco, Ketianthidium zanolae Urban, 2000 e Epanthidium bolivianum.

  1. A new species of Eufriesea Cockerell (Hymenoptera, Apidae, Euglossina from northeastern Brazil

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    Luiz R. R. Faria

    2011-03-01

    Full Text Available A new species of Eufriesea Cockerell (Hymenoptera, Apidae from northeastern Brazil. Eufriesea pyrrhopyga sp. nov. a short-tongued Eufriesea is described as a new species. It can be easily recognized for its predominantly violet lower frons and thorax, violet tergum 1 contrasting with the strong reddish coloration on the lateral portions of terga 2 to 4 and on entire terga 5 and 6, and head pubescence with contrasting colors, white on the lower two-thirds of the face and black on upper frons and vertex. This new species, collected in Recife (Pernambuco, Brazil, apparently is restricted to the Pernambuco endemic center, and seems to be highly endangered.

  2. Five new species of Meteorus Haliday (Hymenoptera: Braconidae: Euphorinae) from Brazil.

    Science.gov (United States)

    De Almeida, Luis Felipe Ventura; Dias, Angélica Maria Penteado

    2015-12-10

    Meteorus Haliday, 1835 (Hymenoptera: Braconidae) is a cosmopolitan genus with around 340 species described, all koinobiont endoparasitoids of Coleoptera or Lepidoptera larvae, and several of its hosts are pest insects. Previously to this work only two species were described from Brazil, M. eaclidis Muesebeck and M. townsendi Muesebeck. Five new species of Meteorus are here described: M. atlanticus n. sp., M. ferruginosus n. sp., M. itatiaiensis n. sp., M. monoceros n. sp., and M. strigatus n. sp. Three species are recorded for the first time from Brazil: M. jerodi Aguirre & Shaw, M. laphygmae Viereck and M. megalops Zitani.

  3. Ciclo de desarrollo de Trigona (Tetragonisca angustula, Latreille 1811 (Hymenoptera, Trigonini

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    María Giomar Nates Parra

    1989-01-01

    Full Text Available Se estudió el ciclo de desarrollo de una abeja sin aguijón: Trigona (Tetragonisca angustula. Latreille 1811 (Hymenoptera. Trigonini. Los resultados fueron los siguientes: El periodo comprendido entre la postura del huevo y la emergencia del imago, es, en las obreras, de 36.5 días en promedio. La duración del ciclo se hace mayor a medida que las celdas se alejan del centro del panal. Se determinaron 3 instares larvales y 5 fases pupales para obreras.

  4. Dopluise (Hemiptera: Coccoidea geassosieer met die wipstertmier, Crematogaster peringueyi Emery (Hymenoptera: Formicidae

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    Johannes H. Giliomee

    2015-02-01

    Full Text Available Neste van die wipstertmier, Crematogaster peringueyi (Hymenoptera: Formicidae, is op verskeie plekke langs die kus van die Wes-Kaap versamel. Die doel was om vas te stel watter dopluisagtiges (Hemiptera: Coccoidea in die neste in assosiasie met hierdie miere leef. Dopluise van drie families, naamlik die Pseudococcidae (witluise, Coccidae (sagtedopluise en Kerriidae (lakdopluise is in die neste gevind, almal bekend daarvoor dat hulle heuningdou afskei. Hierdie mutualistiese verhouding tussen die miere en dopluise, bekend as mirmekofilie, is fakultatief van aard. Die wipstertmier blyk ook nie spesifiek te wees wat betref die plant waarop hulle nes maak nie.

  5. Larvae and Nests of Aculeate Hymenoptera (Hymenoptera: Aculeata) Nesting in Reed Galls Induced by Lipara spp. (Diptera: Chloropidae) with a Review of Species Recorded. Part II.

    Science.gov (United States)

    Astapenková, Alena; Heneberg, Petr; Bogusch, Petr

    2017-01-01

    The ability of aculeate Hymenoptera to utilize wetlands is poorly understood, and descriptions of their nests and developmental stages are largely absent. Here we present results based on our survey of hymenopterans using galls induced by Lipara spp. flies on common reed Phragmites australis in the years 2015-2016. We studied 20,704 galls, of which 9,446 were longitudinally cut and the brood from them reared in the laboratory, while the remaining 11,258 galls reared in rearing bags also in laboratory conditions. We recorded eight species that were previously not known to nest in reed galls: cuckoo wasps Chrysis rutilans and Trichrysis pumilionis, solitary wasps Stenodynerus chevrieranus and Stenodynerus clypeopictus, and bees Pseudoanthidium tenellum, Stelis punctulatissima, Hylaeus communis and Hylaeus confusus. Forty five species of Hymenoptera: Aculeata are known to be associated with reed galls, of which 36 make their nests there, and the other are six parasitoids of the family Chrysididae and three cuckoo bees of the genus Stelis. Of these species, Pemphredon fabricii and in southern Europe also Heriades rubicola are very common in reed galls, followed by Hylaeus pectoralis and two species of the genus Trypoxylon. We also found new host-parasite associations: Chrysis angustula in nests of Pemphredon fabricii, Chrysis rutilans in nests of Stenodynerus clypeopictus, Trichrysis pumilionis in nests of Trypoxylon deceptorium, and Stelis breviuscula in nests of Heriades rubicola. We provide new descriptions of the nests of seven species nesting in reed galls and morphology of mature larvae of eight species nesting in reed galls and two parasitoids and one nest cleptoparasite. The larvae are usually very similar to those of related species but possess characteristics that make them easy to distinguish from related species. Our results show that common reeds are not only expansive and harmful, but very important for many insect species associated with habitats

  6. Hornets Have It: A Conserved Olfactory Subsystem for Social Recognition in Hymenoptera?

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    Antoine Couto

    2017-06-01

    Full Text Available Eusocial Hymenoptera colonies are characterized by the presence of altruistic individuals, which rear their siblings instead of their own offspring. In the course of evolution, such sterile castes are thought to have emerged through the process of kin selection, altruistic traits being transmitted to following generation if they benefit relatives. By allowing kinship recognition, the detection of cuticular hydrocarbons (CHCs might be instrumental for kin selection. In carpenter ants, a female-specific olfactory subsystem processes CHC information through antennal detection by basiconic sensilla. It is still unclear if other families of eusocial Hymenoptera use the same subsystem for sensing CHCs. Here, we examined the existence of such a subsystem in Vespidae (using the hornet Vespa velutina, a family in which eusociality emerged independently of ants. The antennae of both males and female hornets contain large basiconic sensilla. Sensory neurons from the large basiconic sensilla exclusively project to a conspicuous cluster of small glomeruli in the antennal lobe, with anatomical and immunoreactive features that are strikingly similar to those of the ant CHC-sensitive subsystem. Extracellular electrophysiological recordings further show that sensory neurons within hornet basiconic sensilla preferentially respond to CHCs. Although this subsystem is not female-specific in hornets, the observed similarities with the olfactory system of ants are striking. They suggest that the basiconic sensilla subsystem could be an ancestral trait, which may have played a key role in the advent of eusociality in these hymenopteran families by allowing kin recognition and the production of altruistic behaviors toward relatives.

  7. Genes underlying reproductive division of labor in termites, with comparisons to social Hymenoptera

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    Judith eKorb

    2016-04-01

    Full Text Available All social insects are characterized by a reproductive division of labor. Within a colony only a few individuals reproduce (queens and in termites, also a king while the large majority (workers and soldiers forgo reproduction, at least temporarily. The evolution of such reproductive altruism can ultimately be explained by inclusive fitness theory. Here, I will review the proximate genetic mechanisms underlying this altruism in termites. As social cockroaches they evolved eusociality independently from the social Hymenoptera, which makes them interesting test cases to look for common underlying mechanisms of eusociality and lineage specific idiosyncrasies. First, I will provide a summary of the genes and their function that have been identified to underlie reproductive division of labor - so called 'queen genes,' - in the drywood termite Cryptotermes secundus, an emerging model to study termite social evolution. Second, I outline how widespread these queen genes are across the termite phylogeny, using also evidence from recent genome analyses. I will provide hypotheses about the evolutionary origin of these queen genes, aiming to link proximate mechanisms with ultimate functions. Finally, I will draw comparisons to social Hymenoptera to indicate potential common underpinnings that warrant further testing.

  8. A hymenopterists’ guide to the Hymenoptera Anatomy Ontology: utility, clarification, and future directions

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    Katja Seltmann

    2012-05-01

    Full Text Available Hymenoptera exhibit an incredible diversity of phenotypes, the result of ~240 million years of evolution and the primary subject of more than 250 years of research. Here we describe the history, development, and utility of the Hymenoptera Anatomy Ontology (HAO and its associated applications. These resources are designed to facilitate accessible and extensible research on hymenopteran phenotypes. Outreach with the hymenopterist community is of utmost importance to the HAO project, and this paper is a direct response to questions that arised from project workshops. In a concerted attempt to surmount barriers of understanding, especially regarding the format, utility, and development of the HAO, we discuss the roles of homology, “preferred terms”, and “structural equivalency”. We also outline the use of Universal Resource Identifiers (URIs and posit that they are a key element necessary for increasing the objectivity and repeatability of science that references hymenopteran anatomy. Pragmatically, we detail a mechanism (the “URI table” by which authors can use URIs to link their published text to the HAO, and we describe an associated tool (the “Analyzer” to derive these tables. These tools, and others, are available through the HAO Portal website (http://portal.hymao.org. We conclude by discussing the future of the HAO with respect to digital publication, cross-taxon ontology alignment, the advent of semantic phenotypes, and community-based curation.

  9. The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae, a parasitoid wasp of Drosophila

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    Daniel S. Oliveira

    Full Text Available Abstract The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced and a partial A+T-rich region. All protein-coding genes start with ATN codons. Eleven protein-coding genes presented TAA stop codons, whereas ND6 and COII that presented TA, and T nucleotides, respectively. The gene pattern revealed extensive rearrangements compared to the typical pattern generally observed in insects. These rearrangements involve two protein-coding and two ribosomal genes, along with the 16 tRNA genes. This gene order is different from the pattern described for Ibalia leucospoides (Ibaliidae, Cynipoidea, suggesting that this particular gene order can be variable among Cynipoidea superfamily members. A maximum likelihood phylogenetic analysis of the main groups of Apocrita was performed using amino acid sequence of 13 protein-coding genes, showing monophyly for the Cynipoidea superfamily within the Hymenoptera phylogeny.

  10. Integrating age in the detection and mapping of incongruous patches in coffee (Coffea arabica) plantations using multi-temporal Landsat 8 NDVI anomalies

    Science.gov (United States)

    Chemura, Abel; Mutanga, Onisimo; Dube, Timothy

    2017-05-01

    The development of cost-effective, reliable and easy to implement crop condition monitoring methods is urgently required for perennial tree crops such as coffee (Coffea arabica), as they are grown over large areas and represent long term and higher levels of investment. These monitoring methods are useful in identifying farm areas that experience poor crop growth, pest infestation, diseases outbreaks and/or to monitor response to management interventions. This study compares field level coffee mean NDVI and LSWI anomalies and age-adjusted coffee mean NDVI and LSWI anomalies in identifying and mapping incongruous patches across perennial coffee plantations. To achieve this objective, we first derived deviation of coffee pixels from the global coffee mean NDVI and LSWI values of nine sequential Landsat 8 OLI image scenes. We then evaluated the influence of coffee age class (young, mature and old) on Landsat-scale NDVI and LSWI values using a one-way ANOVA and since results showed significant differences, we adjusted NDVI and LSWI anomalies for age-class. We then used the cumulative inverse distribution function (α ≤ 0.05) to identify fields and within field areas with excessive deviation of NDVI and LSWI from the global and the age-expected mean for each of the Landsat 8 OLI scene dates spanning three seasons. Results from accuracy assessment indicated that it was possible to separate incongruous and healthy patches using these anomalies and that using NDVI performed better than using LSWI for both global and age-adjusted mean anomalies. Using the age-adjusted anomalies performed better in separating incongruous and healthy patches than using the global mean for both NDVI (Overall accuracy = 80.9% and 68.1% respectively) and for LSWI (Overall accuracy = 68.1% and 48.9% respectively). When applied to other Landsat 8 OLI scenes, the results showed that the proportions of coffee fields that were modelled incongruent decreased with time for the young age category and

  11. Conversion of nicotinic acid to trigonelline is catalyzed by N-methyltransferase belonged to motif B′ methyltransferase family in Coffea arabica

    International Nuclear Information System (INIS)

    Mizuno, Kouichi; Matsuzaki, Masahiro; Kanazawa, Shiho; Tokiwano, Tetsuo; Yoshizawa, Yuko; Kato, Misako

    2014-01-01

    Graphical abstract: Trigonelline synthase catalyzes the conversion of nicotinic acid to trigonelline. We isolated and characterized trigonelline synthase gene(s) from Coffea arabica. - Highlights: • Trigonelline is a major compound in coffee been same as caffeine is. • We isolated and characterized trigonelline synthase gene. • Coffee trigonelline synthases are highly homologous with coffee caffeine synthases. • This study contributes the fully understanding of pyridine alkaloid metabolism. - Abstract: Trigonelline (N-methylnicotinate), a member of the pyridine alkaloids, accumulates in coffee beans along with caffeine. The biosynthetic pathway of trigonelline is not fully elucidated. While it is quite likely that the production of trigonelline from nicotinate is catalyzed by N-methyltransferase, as is caffeine synthase (CS), the enzyme(s) and gene(s) involved in N-methylation have not yet been characterized. It should be noted that, similar to caffeine, trigonelline accumulation is initiated during the development of coffee fruits. Interestingly, the expression profiles for two genes homologous to caffeine synthases were similar to the accumulation profile of trigonelline. We presumed that these two CS-homologous genes encoded trigonelline synthases. These genes were then expressed in Escherichiacoli, and the resulting recombinant enzymes that were obtained were characterized. Consequently, using the N-methyltransferase assay with S-adenosyl[methyl- 14 C]methionine, it was confirmed that these recombinant enzymes catalyzed the conversion of nicotinate to trigonelline, coffee trigonelline synthases (termed CTgS1 and CTgS2) were highly identical (over 95% identity) to each other. The sequence homology between the CTgSs and coffee CCS1 was 82%. The pH-dependent activity curve of CTgS1 and CTgS2 revealed optimum activity at pH 7.5. Nicotinate was the specific methyl acceptor for CTgSs, and no activity was detected with any other nicotinate derivatives, or with

  12. In vitro AND in vivo ANTHELMINTIC EFFECT OF Coffea arabica RESIDUES AGAINTS AN Haemonchus contortus ISOLATE WITH LOW SUSCEPTIBILITY TO TANNINS

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    G.I. Ortiz-Ocampo

    2016-05-01

    Full Text Available The present study evaluated in vitro and in vivo anthelmintic (AH effect of the percolated by-product of Coffea arabica against a Haemonchus contortus isolate with low susceptibility to tannins. Firstly, the susceptibility to tannins of Paraíso H. contortus isolate was evaluated with the in vitro larval exsheathment inhibition assay (LEIA using an acetone:water (70:30 extract of Acacia pennatula. Afterwards, the in vitro AH effect of acetone:water extracts of percolated C. arabica by-products (Clean and Smooth (CS and Shade Grown (SG Starbucks® were evaluated. Then, the in vivo AH effect was determined in growing sheep (4 months old and 15.2 kg bodyweight, infected with H. contortus (Paraiso isolate and fed with a diet containing the percolated by-product of C. arabica. Three experimental groups were formed (n = 6 lambs: GC Group (control diet without C. arabica, GCA Group (feed containing 10% C. arabica and, GCA+PEG (polyethilenglycol Group (feed with 10% C. arabica + PEG as a tannin inhibitor. Fecal samples were obtained daily from the rectum of lambs (days 1 to 20 of the study. The H. contortus isolate tolerated the A. pennatula extract at concentrations of 150 and 300 μg extract/ml PBS (P > 0.05, which confirmed its low susceptibility to tannins. The C. arabica CS extract reduced the exsheatment from 150 μg of extract/ml PBS and the SG variety from 1200 μg of extract/ml PBS (P0.05. In conclusion, the C. arabica percolated by-product extracts showed in vitro AH effect against exsheathment. However, the consumption of feed containing 10% of the percolated by-product did not show an in vivo AH effect in sheep infected with the Paraiso H. contortus isolate. The latter could be due to the low susceptibility towards the secondary compounds of tannin rich plants of the H. contortus isolate used in this study.

  13. Conversion of nicotinic acid to trigonelline is catalyzed by N-methyltransferase belonged to motif B′ methyltransferase family in Coffea arabica

    Energy Technology Data Exchange (ETDEWEB)

    Mizuno, Kouichi, E-mail: koumno@akita-pu.ac.jp [Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, Akita 010-0195 (Japan); Matsuzaki, Masahiro [Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, Akita 010-0195 (Japan); Kanazawa, Shiho [Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, Bunkyo-ku, Tokyo 112-8610 (Japan); Tokiwano, Tetsuo; Yoshizawa, Yuko [Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, Akita 010-0195 (Japan); Kato, Misako [Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, Bunkyo-ku, Tokyo 112-8610 (Japan)

    2014-10-03

    Graphical abstract: Trigonelline synthase catalyzes the conversion of nicotinic acid to trigonelline. We isolated and characterized trigonelline synthase gene(s) from Coffea arabica. - Highlights: • Trigonelline is a major compound in coffee been same as caffeine is. • We isolated and characterized trigonelline synthase gene. • Coffee trigonelline synthases are highly homologous with coffee caffeine synthases. • This study contributes the fully understanding of pyridine alkaloid metabolism. - Abstract: Trigonelline (N-methylnicotinate), a member of the pyridine alkaloids, accumulates in coffee beans along with caffeine. The biosynthetic pathway of trigonelline is not fully elucidated. While it is quite likely that the production of trigonelline from nicotinate is catalyzed by N-methyltransferase, as is caffeine synthase (CS), the enzyme(s) and gene(s) involved in N-methylation have not yet been characterized. It should be noted that, similar to caffeine, trigonelline accumulation is initiated during the development of coffee fruits. Interestingly, the expression profiles for two genes homologous to caffeine synthases were similar to the accumulation profile of trigonelline. We presumed that these two CS-homologous genes encoded trigonelline synthases. These genes were then expressed in Escherichiacoli, and the resulting recombinant enzymes that were obtained were characterized. Consequently, using the N-methyltransferase assay with S-adenosyl[methyl-{sup 14}C]methionine, it was confirmed that these recombinant enzymes catalyzed the conversion of nicotinate to trigonelline, coffee trigonelline synthases (termed CTgS1 and CTgS2) were highly identical (over 95% identity) to each other. The sequence homology between the CTgSs and coffee CCS1 was 82%. The pH-dependent activity curve of CTgS1 and CTgS2 revealed optimum activity at pH 7.5. Nicotinate was the specific methyl acceptor for CTgSs, and no activity was detected with any other nicotinate derivatives, or

  14. Melhoramento do cafeeiro: XXXVIII. Observações sobre progênies do cultivar Mundo-Novo de Coffea arabica na estação experimental de Mococa Coffee breeding: XXXVIII-observation on progenies of the Mundo-Novo cultivars of Coffea arabica in the Mococa experimental station

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    Túlio R. Rocha

    1980-01-01

    Full Text Available Os dados analisados no experimento localizado em Mococa sobre a produtividade de 112 progênies dos cultivares Mundo-Novo S1 e S2, Bourbon-Amarelo, BourbonVermelho e Caturra-Vermelho de Coffea arabica no período de 1955 a 1971, indicaram que as de Mundo-Novo S1, de prefixos MP 474, MP 502, MP 469, MP 492 e MP 475, revelaram-se como as mais produtivas, assemelhando-se a algumas progênies 'Mundo--Novo' S2. Dentre estas, destacou-se a de prefixo MP 388-6, que atingiu o nível mais elevado de produção do experimento. As progênies de 'Mundo-Novo', em conjunto, produziram 44% a mais do que as de Bourbon-Amarelo e, estas, 60% a mais do que as de Bourbon-Vermelho e Caturra-Vermelho. A altura e o diâmetro da copa atingiram valores médios mais elevados para as progênies de 'Mundo-Novo'. Verificaram-se correlações positivas e altamente significativas entre altura média da planta e diâmetro médio da copa com a produção das progênies. As progênies mais produtivas revelaram rendimento (relação entre peso de café maduro e beneficiado de aproximadamente 6,0 e porcentagem de sementes normais, do tipo chato, acima de 80. Quanto ao tamanho das sementes do tipo chato, duas progênies 'Mundo-Novo' S1, MP 474 e MP 452, apresentaram peneira média maior, permi-tindo seleção de plantas com essa característica e com elevada produção.Coffee progenies of the Mundo-Novo cultivars of Coffea arabica were studied in an experiment located at the Mococa Experimental Station of the Instituto Agronômico in comparison with Bourbon-Amarelo, Bourbon-Vermelho and Caturra-Vermelho cultivars of the same species. During a period of 17 consecutive cropping years (1955-1971, Mundo-Novo yielded approximately 44% more than Bourbon-Amarelo and this cultivars yielded 60% more than Bourbon-Vermelho and Caturra-Vermelho. Among the 89 S1 'Mundo-Novo' progenies, MP 474, MP 502, MP 469, MP 492 and MP 475 yielded as much as the two best 'Mundo-Novo' S2 progenies. Greater

  15. Proteolytic activities of bacteria, yeasts and filamentous fungi isolated from coffee fruit (Coffea arabica L. = Atividade proteolítica de bactérias, leveduras e fungos filamentosos presentes em grãos de café (Coffea arabica L.

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    Mirian Pereira Rodarte

    2011-07-01

    Full Text Available One hundred forty-four microorganisms previously isolated from coffee fruit (Coffea arabica were grown on casein agar to evaluate their proteolytic activities. Fifty percent of filamentous fungi, 52.5% of bacteria and 2.6% of yeasts were able to secrete proteases. Positiveisolates were further examined in liquid culture for their protease activities by hydrolysis of casein at different pH values (5.0, 7.0 and 9.0 at 30 oC. Bacillus megaterium, B. subtilis, Enterobacteragglomerans, Kurthia sp, Pseudomonas paucimobilis and Tatumella ptyseos demonstrated the highest proteolytic activities at pH 9.0. One yeast isolate, Citeromyces matritensis, had a proteolytic activityof 2.40 U at pH 5.0. Aspergillus dimorphicus, A. ochraceus, Fusarium moniliforme, F. solani, Penicillium fellutanum and P. waksmanii showed the highest activities. Of the bacterial isolates, the highestenzyme activities were observed in B. subtilis 333 (27.1 U, Tatumella ptyseos (27.0 U and B. megaterium 817 (26.2 U. Of the filamentous fungi, Aspergillus ochraceus (48.7 U, Fusarium moniliforme 221 (37.5 U and F. solani 359 (37.4 U had the highest activities at pH 9.0. Este trabalho teve por objetivos avaliar a capacidade de secreção de proteases extracelulares por 144 microrganismos, previamente isoladosde grãos de café (Coffea arabica durante fermentação por via seca, e determinar a atividade das enzimas produzidas. Os microrganismos foram cultivados em ágar-caseína para avaliação da produção de enzimas proteolíticas. Dos 40 isolados de bactéria presentes na amostra, 52,5% apresentaram resultado positivo para o teste. Considerando os 66 isolados de fungos filamentosos, 50% foram capazes de secretar proteases, enquanto que dos 38 isolados de leveduras, apenas 2,6% conseguiram promover a hidrólise da caseína do meio. Os isolados que apresentaram capacidade de secreção de proteases foram, posteriormente, cultivados em meio líquido para a determinação da atividade

  16. Crescimento vegetativo e produção de cafeeiros (Coffea arabica L. recepados em duas épocas, conduzidos em espaçamentos crescentes Vegetative growth and yield of coffee plants (Coffea arabica L. in two different pruning times, conducted at different spacings

    Directory of Open Access Journals (Sweden)

    Sérgio Parreiras Pereira

    2007-06-01

    Full Text Available O experimento foi instalado na Fazenda Experimental da Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG em Machado, Sul de Minas Gerais, em 1992, com o objetivo de avaliar as conseqüências da redução dos espaçamentos entre as linhas e entre as plantas na linha de plantio sobre a produção e a fenologia do cafeeiro(Coffea arabica L.. O delineamento experimental foi o blocos casualizados - DBC, em um arranjo fatorial 4 x 3 com parcela subdividida, sendo quatro distâncias entre as linhas (2,0; 2,5; 3,0 e 3,5 m e três distâncias entre as plantas na linha de plantio (0,5; 0,75; 1,0 m, e duas épocas de poda (uma precoce feita logo após a colheita em de julho 2002 e a outra tardia em de janeiro de 2003, totalizando 24 tratamentos com três repetições. Em julho de 2002 e em janeiro de 2003 foi realizada a poda tipo "recepa", na qual foram conduzidas duas brotações por planta. Em agosto de 2004, avaliou-se o crescimento dos componentes vegetativos e a produção das parcelas. Os espaçamentos adotados não influenciaram o crescimento de nenhum dos componentes vegetativos das brotações no período avaliado. Todas as características vegetativas foram influenciadas positivamente pela adoção da poda precoce, assim como a produtividade da primeira colheita realizada após a poda, que foi também influenciada positivamente pela adoção de espaçamentos mais adensados. Os cafeeiros que foram submetidos à poda tardia não produziram, em julho de 2004, como aqueles podados precocemente.The experiment was established in the Empresa de Pesquisa Agropecuária de Minas Gerais - Epamig Experiment Station, located in the city of Machado, south of the Minas Gerais state, Brazil, in the year of 1992, with the objective of evaluating the consequences of the reduction on planting spaces among rows and among plants, upon beans yield and plant phenology (Coffea arabica L.. The experimental design used was a 4x 3 factorial with split plot at

  17. Registro de nido de Camponotus rufipes (Formicidae: Hymenoptera en un armario metálico dentro de una estructura urbana | Nesting report of Camponotus rufipes (Formicidae: Hymenoptera in a metallic cabinet insight an urban structure

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    Cristina Sainz-Borgo

    2017-11-01

    Full Text Available Urban ants are common and have a great importance for humans, for the effects in the houses or for being vectors of pathogens. The present paper reports the presence of a nest of Camponotus atriceps (Formicidae: Hymenoptera inside a metal cabinet in a research laboratory at Simón Bolívar University (Caracas, Venezuela. This report constitutes one of the few records for this species in metallic structures, since they usually occupy wooden structures.

  18. The origins of species richness in the Hymenoptera: insights from a family-level supertree

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    Davis Robert B

    2010-04-01

    Full Text Available Abstract Background The order Hymenoptera (bees, ants, wasps, sawflies contains about eight percent of all described species, but no analytical studies have addressed the origins of this richness at family-level or above. To investigate which major subtaxa experienced significant shifts in diversification, we assembled a family-level phylogeny of the Hymenoptera using supertree methods. We used sister-group species-richness comparisons to infer the phylogenetic position of shifts in diversification. Results The supertrees most supported by the underlying input trees are produced using matrix representation with compatibility (MRC (from an all-in and a compartmentalised analysis. Whilst relationships at the tips of the tree tend to be well supported, those along the backbone of the tree (e.g. between Parasitica superfamilies are generally not. Ten significant shifts in diversification (six positive and four negative are found common to both MRC supertrees. The Apocrita (wasps, ants, bees experienced a positive shift at their origin accounting for approximately 4,000 species. Within Apocrita other positive shifts include the Vespoidea (vespoid wasps/ants containing 24,000 spp., Anthophila + Sphecidae (bees/thread-waisted wasps; 22,000 spp., Bethylidae + Chrysididae (bethylid/cuckoo wasps; 5,200 spp., Dryinidae (dryinid wasps; 1,100 spp., and Proctotrupidae (proctotrupid wasps; 310 spp.. Four relatively species-poor families (Stenotritidae, Anaxyelidae, Blasticotomidae, Xyelidae have undergone negative shifts. There are some two-way shifts in diversification where sister taxa have undergone shifts in opposite directions. Conclusions Our results suggest that numerous phylogenetically distinctive radiations contribute to the richness of large clades. They also suggest that evolutionary events restricting the subsequent richness of large clades are common. Problematic phylogenetic issues in the Hymenoptera are identified, relating especially to

  19. Prey identification in nests of the potter wasp Hypodynerus andeus (Packard (Hymenoptera, Vespidae, Eumeninae using DNA barcodes

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    Héctor A. Vargas

    2014-06-01

    Full Text Available Prey identification in nests of the potter wasp Hypodynerus andeus (Packard (Hymenoptera, Vespidae, Eumeninae using DNA barcodes. Geometrid larvae are the only prey known for larvae of the Neotropical potter wasp Hypodynerus andeus (Packard, 1869 (Hymenoptera, Vespidae, Eumeninae in the coastal valleys of the northern Chilean Atacama Desert. A fragment of the mitochondrial gene cytochrome oxidase c subunit 1 was amplified from geometrid larvae collected from cells of H. andeus in the Azapa Valley, Arica Province, and used to provide taxonomic identifications. Two species, Iridopsis hausmanni Vargas, 2007 and Macaria mirthae Vargas, Parra & Hausmann, 2005 were identified, while three others could be identified only at higher taxonomic levels, because the barcode reference library of geometrid moths is still incomplete for northern Chile.

  20. Seasonality of Pelecinus polyturator (Drury (Hymenoptera, Pelecinidae in the Atlantic Rainforest of São Paulo State, Brazil

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    Rogéria I. R. Lara

    2014-03-01

    Full Text Available Seasonality of Pelecinus polyturator (Drury (Hymenoptera, Pelecinidae in the Atlantic Rainforest of São Paulo State, Brazil. A survey of the parasitoid wasp Pelecinus polyturator (Drury, 1773 (Hymenoptera, Pelecinidae was carried out with five Malaise traps/area in five areas in the Atlantic Rainforest of São Paulo State, Brazil, between November 2009 and October 2010. The sampling effort in each locality amounted to 1,825 trap-days. Data were obtained from a total of 317 exemplars of P. polyturator, corresponding to 108 females and 209 males. The average sex ratio of the studied population was 0.52. The highest occurrence of P. polyturator was observed between November and March with frequency peak in January; about 95% of the specimens studied were captured at altitudes close to 1,000 m above sea level.

  1. A new species of solitary Meteorus (Hymenoptera: Braconidae) reared from caterpillars of toxic butterflies (Lepidoptera: Nymphalidae) in Ecuador.

    Science.gov (United States)

    Shaw, Scott R; Jones, Guinevere Z

    2009-01-01

    A new species of parasitoid wasp, Meteorus rugonasus Shaw and Jones (Hymenoptera: Braconidae), is described from the Yanayacu Biological Station, Napo Province, Ecuador. The new species is diagnosed and compared to other species in the genus. It was reared from larvae of Pteronymia zerlina (Hewitson, 1855) (Lepidoptera: Nymphalidae, Ithomiinae) found feeding on leaves of Solanum (Solanaceae). The parasitoid is solitary. This is the first record of a Meteorus species attacking ithomiine Nymphalidae. A new species of parasitoid wasp, Meteorus rugonasus Shaw and Jones (Hymenoptera: Braconidae), is described from the Yanayacu Biological Station, Napo Province, Ecuador. The new species is diagnosed and compared to other species in the genus. It was reared from larvae of Pteronymia zerlina (Hewitson, 1855) (Lepidoptera: Nymphalidae, Ithomiinae) found feeding on leaves of Solanum (Solanaceae). The parasitoid is solitary. This is the first record of a Meteorus species attacking ithomiine Nymphalidae.

  2. Predation of Apiomerus pilipes (Fabricius (Hemiptera, Reduviidae, Harpactorinae, Apiomerini over Meliponinae bees (Hymenoptera, Apidae, in the State of Amazonas, Brazil

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    Alexandre Coletto da Silva

    2004-12-01

    Full Text Available The present work shows the occurrence of an intense predatory activity on adults working Meliponinae bees (Hymenoptera, Apidae, by Apiomerus pilipes (Fabricius, 1787 (Hemiptera, Reduviidae, Harpactorinae, Apiomerini at a meliponary in the Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas State, Brazil.O presente trabalho registra a ocorrência de intensa atividade predatória de Apiomerus pilipes (Fabricius, 1787 (Hemiptera, Reduviidae, Harpactorini, Apiomerini sobre operárias adultas de meliponíneos (Hymenoptera, Apidae, no meliponário experimental do Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Estado do Amazonas, Brasil. O meliponário se encontra num fragmento de vegetação secundária no próprio INPA.

  3. Higher-level bee classifications (Hymenoptera, Apoidea, Apidae sensu lato Classificação dos grandes grupos de abelhas (Hymenoptera, Apoidea, Apidae sensu lato

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    Gabriel A. R. Melo

    2005-03-01

    Full Text Available A higher-level classification of bees, in which the entire group is treated as a single family - the Apidae - is advocated here. A total of seven subfamilies, 51 tribes and 27 subtribes are recognized. These subfamilies correspond to the families adopted in the traditional classification. Although the proposed changes do not involve any major rearrangement, basically only changing the rank given to the main groups, the new system makes the classification of bees more consistent with that adopted for other major groups of aculeate Hymenoptera. It also departs from the 19th century practice, perpetuated in the traditional classification, of giving family-status to the main groups of bees. A correspondence table associating the taxon names used in the current traditional classification with those of the revised classification is presented. Scrapterini new tribe (type-genus Scrapter Lepeletier & Serville is proposed to accommodate the southern African genus Scrapter.Apresenta-se uma classificação para as abelhas em que o todo o grupo é tratado como uma única família - Apidae. São reconhecidas sete subfamílias, 51 tribos e 27 subtribos. As subfamílias correspondem às famílias da classificação tradicional. Apesar das mudanças propostas afetarem apenas o status dos grupos, o novo sistema torna a classificação das abelhas mais consistente com aquela adotada para os grandes grupos de Hymenoptera aculeados. Além disso, distancia-se da tradição de dar status de família aos grupos principais de abelhas, uma prática do século 19 perpetuada na classificação tradicional. É apresentada uma tabela de correspondência associando os nomes dos táxons usados na classificação tradicional corrente com aquelas da classificação sendo proposta aqui. Scrapterini tribo nova (gênero-tipo Scrapter Lepeletier & Serville é proposta para acomodar Scrapter, um gênero restrito à porção sul do continente africano.

  4. The discovery of the genus Spasskia Belokobylskij, 1989 (Hymenoptera: Braconidae) in China, with description of a new species.

    Science.gov (United States)

    Yan, Cheng-jin; He, Jun-hua; Chen, Xue-xin

    2014-01-01

    The genus Spasskia Belokobylskij, 1989 (Hymenoptera: Braconidae: Helconinae) is reported for the first time from China. Two species, namely Spasskia brevicarinata Yan et Chen sp. n.and Spasskia indica Singh, Belokobylskij et Chauhan, 2005 are described and illustrated. A key to the species of this genus is updated to include the new species. This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.

  5. Temporal Activity Patterns of the Spider Wasp Pepsis montezuma Smith (Hymenoptera: Pompilidae) in a Disturbed Lower Montane Rainforest (Manizales, Colombia)

    OpenAIRE

    Restrepo-Giraldo, Carlos; Rodriguez, Juanita; Pitts, James P.

    2012-01-01

    We studied the temporal activity pattern of the spider wasp Pepsis montezuma Smith (Hymenoptera: Pompilidae) in a disturbed lower montane rainforest, which is located in the city of Manizales, Colombia, at an altitude of 2,150 m. Females of this species are diurnal with two peaks of activity: one in the morning and the other in the afternoon. During the morning, nectar foraging occurred at Baccharis latifolia. During the afternoon, females hunted for tarantulas of the genus Pamphobeteus (Aran...

  6. Risk of anaphylaxis in patients with large local reactions to hymenoptera stings: a retrospective and prospective study.

    Science.gov (United States)

    Pucci, Stefano; D'Alò, Simona; De Pasquale, Tiziana; Illuminati, Ilenia; Makri, Elena; Incorvaia, Cristoforo

    2015-01-01

    In the few studies available, the risk of developing systemic reactions (SR) to hymenoptera stings in patients with previous large local reactions (LLRs) to stings ranges from 0 to 7 %. We evaluated both retrospectively and prospectively the risk of SRs in patients with LLRs to stings. An overall number of 477 patients, 396 with an SR as the first manifestation of allergy and 81 with a history of only LLRs after hymenoptera stings, were included in the study. All patients had clinical history and allergy testing (skin tests and/or specific IgE) indicative of allergy to venom of only one kind of Hymenoptera. Of the 81 patient with LLRs, 53 were followed-up for 3 years by annual control visits, while the 396 patients with SR were evaluated retrospectively. Among the 396 patients with an SR, only 17 (4.2 %) had had a previous LLR as debut of allergy, after an history of normal local reactions to Hymenoptera stings. All the 81 patients with a history of only LLRs had previously had at least two LLRs, with an overall number of 238 stings and no SR. Among the 53 patients who were prospectively evaluated we found that 31 of them (58.3 %) were restung by the same type of insect, with an overall number of 59 stings, presenting only LLRs and no SR. Our findings confirm that patients with repeated LLRs to stings had no risk of SR, while a single LLR does not exclude such risk. This has to be considered in the management of patients with LLRs.

  7. Impact of Hymenoptera venom allergy and the effects of specific venom immunotherapy on mast cell metabolites in sensitized children

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    Ewa Cichocka-Jarosz

    2014-06-01

    Full Text Available introduction and objective. Mast cells (MC are effector cells during severe systemic reactions (SR to Hymenoptera stings. Venom specific immunotherapy (VIT is the treatment of choice for prevention of SR to stings. Tryptase and prostaglandin D[sub]2[/sub] metabolites (PGD[sub]2[/sub] are the markers of MC activation. The study design was to 1. compare baseline values of serum tryptase concentration (BST and PGD[sub]2[/sub] metabolites in children with/without venom sensitization, 2. to evaluate an influence of rush VIT on MC markers in treated children. materials and methods. Sensitized group: 25 children with SR to Hymenoptera sting. Control group: 19 healthy children. Active treatment: 5-day-rush-VIT. BST was evaluated by ImmunoCAP, PGD[sub]2[/sub] metabolites in blood and urine by GC-NICI-MS. results. The baseline blood levels of MC markers were significantly higher, while urinary concentration of 9α,11β-PGF2 was significantly lower in the whole group of venom-sensitized children compared to controls. Severity of SR showed negative correlation with urinary PGD[sub]2[/sub] metabolites, while positive with plasma 9α,11β-PGF2 and BST concentration The highest sensitivity was obtained for plasma 9α,11β-PGF2 whereas the highest specificity for urinary PGD-M. conclusions. In children with IgE-mediated SR to Hymenoptera stings, elevation of baseline values of PGD2 metabolites in blood is accompanied by decreased excretion of its urinary metabolites. Assessment of stable PGD[sub]2 [/sub] metabolites might serve as an independent MC marker to identify allergic children. There is an association between urinary PGD[sub]2[/sub] metabolites and severity of the SR to Hymenoptera stings.

  8. Catalogue of Danish Alysiinae (Hymenoptera: Braconidae, with the description of two new species of Aspilota Foerster, 1863

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    Francisco Javier Peris-Felipo

    2016-12-01

    Full Text Available In the present study, a total of 153 species of Alysiinae (Hymenoptera: Braconidae from Denmark are catalogued. Two species are described as new for science: Aspilota leptoarticulata Munk & Peris-Felipo sp. nov. and A. grandis Munk & Peris-Felipo sp. nov. Additionally, 38 alysiine species are recorded for the first time for the Danish fauna. A faunistic list with distribution data and host records is provided.

  9. A New Species of Solitary Meteorus (Hymenoptera: Braconidae) Reared from Caterpillars of Toxic Butterflies (Lepidoptera: Nymphalidae) in Ecuador

    OpenAIRE

    Shaw, Scott R.; Jones, Guinevere Z.

    2009-01-01

    A new species of parasitoid wasp, Meteorus rugonasus Shaw and Jones (Hymenoptera: Braconidae), is described from the Yanayacu Biological Station, Napo Province, Ecuador. The new species is diagnosed and compared to other species in the genus. It was reared from larvae of Pteronymia zerlina (Hewitson, 1855) (Lepidoptera: Nymphalidae, Ithomiinae) found feeding on leaves of Solanum (Solanaceae). The parasitoid is solitary. This is the first record of a Meteorus species attacking ithomiine Nympha...

  10. Evaluation of the quality of life in subjects with a history of severe anaphylactic reaction to the Hymenoptera venom.

    Science.gov (United States)

    Nowak, Natalia; Bazan-Socha, Stanisława; Pulka, Grażyna; Pełka, Karolina; Latra, Paulina

    2015-01-01

    Sensitization to the Hymenoptera venom is one of the main causes of anaphylaxis in Poland. Venom immunotherapy is the only effective treatment in such cases. Comprehensive patient care includes also education. The aim of our study was to assess the state of knowledge and to evaluate the quality of life and the anxiety level in patients allergic to the Hymenoptera venom after anaphylactic reaction. The survey was carried out in the period of the insects flight in 61 adult subjects (35 wasp and 26 bee allergic), using a validated Vespid Allergy Quality of Life Questionnaire (VQLQ), Hospital Anxiety and Depression Scale, and subjective assessment of anxiety level. The majority of respondents received venom immunotherapy. Sensitized to the wasp venom had significantly impaired quality of life (VQLQ score) as compared to the bee venom allergic (p = 0.014). The intensity of anxiety decreased with the duration of immunotherapy (p = 0.01). The majority of subjects knew how to recognize and treat anaphylaxis, but only 8% employed an identification card and about 50% implemented rules of the pre-exposition prophylaxis. History of a severe anaphylaxis to the Hymenoptera venom affected the quality of life. Venom immunotherapy reduced anxiety. We hope that presented surveys and their results might be useful in qualifying for immunotherapy in clinically uncertain cases.

  11. Ten unique and charismatic new species of Microgastrinae wasps (Hymenoptera, Braconidae from North America

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    Jose Fernandez-Triana

    2018-01-01

    Full Text Available Ten new species within four genera of Microgastrinae parasitoid wasps (Hymenoptera: Braconidae are described from Canada and United States: Diolcogaster ichiroi, Diolcogaster miamensis, Glyptapanteles pseudotsugae, Microgaster archboldensis, Microgaster syntopic, Microplitis altissimus, Microplitis jorgeluisi, Microplitis juanmanueli, Microplitis julioalbertoi, and Microplitis mariamargaritae. The new taxa are significant because they represent the first North American records of a tropical group (species of the basimacula group in Diolcogaster, exemplify interesting ecological cases (niche-based host selection in Glyptapanteles, syntopic species in Microgaster, and showcase unique morphological features and/or altitudinal records (Microplitis. Most of the new species were collected in protected areas or areas with strong research programs (Archbold Biological Station and hammock forests near Miami, Florida; Great Sand Dunes National Park and Preserve, and Mount Evans Wilderness Area, Colorado; Sapelo Island, Georgia; Tonto National Forest, Arizona, and thus are also of value and interest for conservation and research efforts.

  12. Description of five species of Xanthopimpla Saussure 1892 (Hymenoptera: Ichneumonidae: Pimplinae) from Malaysia

    Science.gov (United States)

    Dass, Angeline David; Ghani, Idris Abd.

    2013-11-01

    Description of five species of Xanthopimpla Saussure, 1829 (Hymenoptera: Ichneumonidae: Pimplinae) from Malaysia was done using specimens deposited in Centre for Insects Systematics, Universiti Kebangsaan Malaysia (CIS, UKM). Type and non-type specimens were loaned from several repositories namely Zoological Museum of Amsterdam Netherlands (ZMAN), Swedish Museum of Natural History (NRM), British Natural History Museum London (BMNH) and Department of Agricultural Malaysia (DOA) for identification and comparison. The specimens were identified to the species level which gives rise to five species namely Xanthopimpla conica Cushman, 1925, Xanthopimpla despinosa leipephelis Townes & Chiu, 1970, Xanthopimpla flavolineata Cameron, 1907, Xanthopimpla punctata (Fabricius, 1781) and Xanthopimpla tricapus impressa Townes & Chiu, 1970. A dichotomous key and descriptions for five Xanthopimpla spesies were provided. Photos and illustrations of carina on propodeum were also included in this paper.

  13. Antibacterial Compounds from Propolis of Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae) from Thailand

    Science.gov (United States)

    Sanpa, Sirikarn; Popova, Milena; Bankova, Vassya; Tunkasiri, Tawee; Eitssayeam, Sukum; Chantawannakul, Panuwan

    2015-01-01

    This study investigated the chemical composition and antimicrobial activity of propolis collected from two stingless bee species Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae). Six xanthones, one triterpene and one lignane were isolated from Tetragonula laeviceps propolis. Triterpenes were the main constituents in T. melanoleuca propolis. The ethanol extract and isolated compounds from T. laeviceps propolis showed a higher antibacterial activity than those of T. melanoleuca propolis as the constituent α-mangostin exhibited the strongest activity. Xanthones were found in propolis for the first time; Garcinia mangostana (Mangosteen) was the most probable plant source. In addition, this is the first report on the chemical composition and bioactivity of propolis from T. melanoleuca. PMID:25992582

  14. Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis.

    Science.gov (United States)

    Cruz, L C; Araújo, V A; Dolder, H; Araújo, A P A; Serrão, J E; Neves, C A

    2011-01-01

    In Hymenoptera, midgut changes begin in the last instar. At this stage, the larval epithelial digestive cells degenerate, leaving only the basal membrane and the regenerative cells which will develop into a new epithelium during the pupal stage and in the adult. Epithelium renewal is followed by changes in volume and shape of the midgut. Morphometric analysis of digestive cells and total midgut volume of Melipona quadrifasciata anthidioides (Lepeletier) were conducted to verify whether cell volume increase are sufficient to account for the total midgut volume increase that occurs during metamorphosis. An increase in midgut volume was verified in spite of the scarcity of cell proliferation found during metamorphosis. At the end of metamorphosis, the increase in cell volume was not sufficient to explain the increase in volume of the midgut, indicating that an increase in the number of digestive cells is apparently necessary. Nevertheless, the mechanism by which regenerative cells reconstitute the epithelium during metamorphosis remains unknown.

  15. Nesting sites characteristics of stingless bees (Hymenoptera: Apidae in Central Sulawesi, Indonesia

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    Nelky Suriawanto

    2017-06-01

    Full Text Available Stingless bees (Hymenoptera: Apidae is eusocial insects that live together in a colony. This research was aimed to study the nesting site characteristics of stingless bees in the settlement areas at Central Sulawesi, Indonesia. The nesting sites were observed by purposive sampling method from July 2015 to January 2016. Four species belong to genus Tetragonula were found, namely T. fuscobalteata, T. biroi, T. sapiens, and T. laeviceps. Two spesies, T. biroi and T. sapiens are the new record in Sulawesi island. The highest abundance of stingless bees colony was T. fuscobalteata (92.26%, followed by T. biroi (4.17%, T. sapiens (2.98%, and T. laeviceps (0.59%. Nesting sites of T. fuscobalteata were found in the stone, brick wall, wooden wall, bamboo, and iron cavities, T. biroi in the wooden wall, stone, and brick wall cavities, T. sapiens in stone cavities, while T. laeviceps in wooden walls.

  16. A new mesoserphid wasp from the Middle Jurassic of northeastern China (Hymenoptera, Proctotrupoidea

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    Yan Zheng

    2017-12-01

    Full Text Available A new genus and species of Mesoserphidae (Hymenoptera, Juraserphus modicus gen. et sp. nov., is described based on a well-preserved fossil specimen from the Middle Jurassic Jiulongshan Formation of northeastern China. It is characterized by the following forewing features: the forking of Rs+M located approximately one-third of the distance between 1m-cu and 2r-rs, both 1cu-a and 2cu-a antefurcal; 1-M more than twice as long as 1m-cu and hind wing with cells r and rm closed. In addition, it has a short ovipositor, only extending slightly beyond the metasomal apex. Its new morphological characters broaden the diversity of Mesoserphidae in the Mesozoic and provide new insights into the evolution and relationships of Mesoserphidae.

  17. Additions to the Knowledge of the Genus Phimenes (Hymenoptera: Vespidae: Eumeninae from Vietnam

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    Lien Thi Phuong

    2016-01-01

    Full Text Available The solitary wasp genus Phimenes Giordani Soika, 1992 (Hymenoptera: Vespidae: Eumeninae was reported to occur in Vietnam by van der Vecht (1959, represented by one taxon, Phimenes flavopictus continentalis (Zimmermann, which was synonymized under nominotypical Phimenes flavopictus (Blanchard, 1849 by Kumar (2013. A note on gender of this genus is made in the text. One more species, Phimenes indosinensis (van der Vecht, 1959 is recorded in this study from Dak Lak in the southern and Son La in the northwestern parts of Vietnam for the first time. Detailed descriptions of the female and male of the latter are provided with figures. A key to the two species from Vietnam is also provided.

  18. Insecticide toxicity to Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) females and effect on descendant generation.

    Science.gov (United States)

    Vianna, Ulysses R; Pratissoli, Dirceu; Zanuncio, José C; Lima, Eraldo R; Brunner, Jay; Pereira, Fabrício F; Serrão, José E

    2009-02-01

    The effect of nine insecticides used in tomato production was evaluated on adults of two Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) populations from Rive and Afonso Cláudio, Espírito Santo State, Brazil. The experiment was developed in an acclimatized chamber at 25 +/- 1 degrees C, 70 +/- 10% relative humidity and 14 h photophase. Eggs of Anagasta kuehniella (Lepidoptera: Pyralidae), previously immersed in insecticides solutions were offered to females of both T. pretiosum populations. Bacillus thuringiensis, lufenuron and triflumuron had lowest negative effects on parasitism and viability of individuals of these populations; however, abamectin and pyrethroids (betacyflurin 50 and 125 g/l and esfenvalerate) insecticides reduced parasitism rates. T. pretiosum emerged from A. kuehniella eggs treated with esfenvalerate but were not able to parasitize non treated eggs of this host. B. thuringiensis, lufenuron and triflumuron may be used in integrated pest management programs to control tomato pests, because they have moderated negative effect on parasitoid wasps.

  19. Revision of the Palaearctic Gasteruption assectator aggregate, with special reference to Sweden (Hymenoptera, Gasteruptiidae

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    Niklas Johansson

    2016-09-01

    Full Text Available The Palaearctic species of the Gasteruption assectator aggregate (Hymenoptera, Gasteruptiidae are revised and three species are recognised. Two species are re-instated: Gasteruption boreale (Thomson, 1883, stat. n. and G. nigritarse (Thomson, 1883, stat. n., and both are excluded from the synonymy with G. assectator (Linnaeus, 1758. The general distribution of both species is given for Europe and in detail for Sweden. A key to the valid Palaearctic species of the Gasteruption assectator aggregate is given; key characters and primary types are illustrated. Four new synonyms are listed: Foenus fumipennis Thomson, 1883, Trichofoenus breviterebrae Watanabe, 1934, and Gasteruption margotae Madl, 1987, are synonymized with Gasteruption boreale (Thomson, 1883 and Gasteruption brevicauda Kieffer, 1904, with G. undulatum (Abeille de Perrin, 1879.

  20. Antibacterial Compounds from Propolis of Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae from Thailand.

    Directory of Open Access Journals (Sweden)

    Sirikarn Sanpa

    Full Text Available This study investigated the chemical composition and antimicrobial activity of propolis collected from two stingless bee species Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae. Six xanthones, one triterpene and one lignane were isolated from Tetragonula laeviceps propolis. Triterpenes were the main constituents in T. melanoleuca propolis. The ethanol extract and isolated compounds from T. laeviceps propolis showed a higher antibacterial activity than those of T. melanoleuca propolis as the constituent α-mangostin exhibited the strongest activity. Xanthones were found in propolis for the first time; Garcinia mangostana (Mangosteen was the most probable plant source. In addition, this is the first report on the chemical composition and bioactivity of propolis from T. melanoleuca.

  1. Development of antennal sensilla of Tetragonisca angustula Latreille, 1811 (Hymenoptera: Meliponini during pupation

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    V. T. Dohanik

    Full Text Available Abstract The antennal sensilla are sensory organs formed by a group of neurons and accessory cells, which allow perception of environmental cues, which play a role as mechanoreceptors and chemoreceptors. This study describes the post-embryonic development of the antennal sensilla of the stingless Tetragonisca angustula (Hymenoptera: Meliponini workers. The development of the antennal sensilla begins in the transition stage of the pre-pupae to white-eyed pupae. The sensilla are completely developed at the black-eyed pupae stage, but they are covered by the old cuticle. The sensilla are exposed to the environment only in newly emerged workers of T. angustula, but it is possible that environmental stimuli can be recognized due to the pores in the old cuticle.

  2. Lymphocyte-mediated regulation of platelet activation during desensitization in patients with hymenoptera venom hypersensitivity.

    Science.gov (United States)

    Ledru, E; Pestel, J; Tsicopoulos, A; Joseph, M; Wallaert, B; Tonnel, A B; Capron, A

    1988-01-01

    T cells from peripheral blood of hymenoptera sensitive patients were studied before and after venom desensitization. Before treatment, T cells showed a variable but higher proliferative response to allergen than T cells of treated patients or controls. While before desensitization, T cell products, specifically released after in vitro allergen stimulation, were able to amplify the IgE-dependent platelet activity, we showed that after treatment of the same patients, T cell products strongly reduced platelet activation. Considering the modifications in platelet activation previously observed in patients treated by specific immunotherapy, the present results suggest that, through a modification of T cell reactivity to allergen, T cell functions are modulated by desensitization, and emphasize the involvement of T cell products in the desensitization mechanisms. PMID:3263227

  3. Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae)

    International Nuclear Information System (INIS)

    Pratissoli, Dirceu; Oliveira, Harley N. de; Oliveira, Regiane C. de; Zago, Hugo B.; Vieira, Stella M.J.

    2004-01-01

    Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae). Biological characteristics of Trichogramma galloi Zucchi, 1988 were evaluated in laboratory where these parasitoids were reared on eggs of Diatraea saccharalis (Lepidoptera, Pyralidae) with or without honey, and exposed to eggs of the host after 0, 6, 12, 24, 36, 48, 60, 72 and 84 hours of emergence. The parasitism rate and viability showed higher for individuals that received food. The sex ratio was not influenced by food. The number of individuals per egg only showed difference for those adults that did not receive food and stayed six hours without the host eggs. Checking the effect of the availability of eggs, only the sex ratio, with or without honey, did not show differences. The results show that T. galloi needs a carbohydrate supply and the time can influence the reproductive capacity. (author)

  4. First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae

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    Thiago S. Montagna

    2012-06-01

    Full Text Available First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae. Parasitism of colonies of the social wasp Mischocyttarus cerberus Ducke, 1918 by females of Mischocyttarus consimilis Zikán, 1949 was observed in a rural area of Dourados, state of Mato Grosso do Sul, Brazil. In all monitored cases, the invasion occurred in the pre-emergence colony stage, generally by a single female of M. consimilis. The period of establishment of the foreign female in the host colony was marked by antagonistic behaviors between the host female and the invasive. In general, the architecture of the parasitized nest was modified from the typical architecture of the host species nest.

  5. Ophioninae (Hymenoptera: Ichneumonidae wasp community in the cloudy forest Monteseco, Cajamarca, Peru

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    Evelyn Sánchez

    2014-12-01

    Full Text Available We describe the species composition of the subfamily Ophioninae (Hymenoptera: Ichneumonidae along an altitudinal gradient in the cloudy forest Monteseco, Cajamarca, Peru collected in 2009 and 2010. Eighteen species were recorded in three genera of Ophioninae: Alophophion, Enicospilus y Ophion. Five species are recorded for the first time in Peru: Ophion polyhymniae Gauld, 1988; Enicospilus cubensis (Norton, 1863; E. guatemalensis (Cameron, 1886; E. cressoni Hooker, 1912 y E. mexicanus (Cresson, 1874. Subfamily composition varies with the elevation. The highest species richness (S=11 was found at 2150 m and the lowest (S=3 at 3116 m. Enicospilus is more diverse from low to mid elevation, Ophion from mid to high elevation and Alophophion occurs predominantly at high elevation.

  6. Notes on the systematics of the orchid-bee genus Eulaema (Hymenoptera, Apidae

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    Gabriel A. R. Melo

    2014-09-01

    Full Text Available Notes on the systematics of the orchid-bee genus Eulaema (Hymenoptera, Apidae. The classification of the genus Eulaema is modified in order to make it congruent with recent phylogenetic hypotheses based on molecular data. The speciosa group, containing E. peruviana, E. speciosa and related species, is removed from E. (Eulaema and transferred to E. (Apeulaema. New morphological characters are presented to support the revised scope of the subgenera and their diagnostic features are revised. Six species groups are recognized herein: two in E. (Apeulaema and four in E. (Eulaema. A list of valid species in each species group and an identification key to males of each of the subgenera and species groups are provided. Finally, an older overlooked designation of a type species for Eulaema is presented in the Appendix.

  7. Dirhinus giffardii (Hymenoptera: Chalcididae, parasitoid affecting Black Soldier Fly production systems in West Africa

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    Emilie Devic

    2015-12-01

    Full Text Available Interest for insect farming is currently growing globally. Conditions in West Africa appear suitable for developing such farming systems that can benefit communities by improving livelihoods, food and feed security or sanitation. In Ghana and Mali, the Black Soldier Fly (Hermetia illucens Linnaeus, 1758 is being produced for waste recycling and animal feed. In a two stages process (egg and larvae production, egg production was hampered by a pupal parasitoid, Dirhinus giffardii Silvestri, 1913 (Hymenoptera: Chalcididae, which reduced future broodstock by almost 72%. This is the first time D. giffardii is reported as a parasitoid of H. illucens pupae and one of the first reports of parasitism in this commercially important fly species. The introduction of precautionary measures is highly recommended for the success of H. illucens production systems in West Africa.

  8. [Coupling effects of periodic rewatering after drought stress and nitrogen fertilizer on growth and water and nitrogen productivity of Coffea arabica].

    Science.gov (United States)

    Hao, Kun; Liu, Xiao Gang; Zhang, Yan; Han, Zhi Hui; Yu, Ning; Yang, Qi Liang; Liu, Yan Wei

    2017-12-01

    The effects of periodic rewatering after drought stress and nitrogen fertilizer on growth, yield, photosynthetic characteristics of leaves and water and nitrogen productivity of Coffea arabica (Katim P7963) were studied under different nitrogen application levels in 2.5 consecutive years. Irrigation (periodic rewatering after drought stress) and nitrogen were designed as two factors, with four modes of irrigation, namely, full irrigation (I F-F : 100%ET 0 +100%ET 0 , ET 0 was reference crop evapotranspiration), rewatering after light drought stress (I L-F : 80%ET 0 +100%ET 0 ), rewatering after moderate drought stress (I M-F : 60%ET 0 +100%ET 0 ) and rewatering after severe drought stress (I S-F : 40%ET 0 +100%ET 0 ), and three levels of nitrogen, namely, high nitrogen (N H : 750 kg N·hm -2 each time), middle nitrogen (N M : 500 kg N·hm -2 each time), low nitrogen (N L : 250 kg N·hm -2 each time), and nitrogen was equally applied for 4 times. The results showed that irrigation and nitrogen had significant effect on plant height, stem diameter, yield and water and nitrogen productivity of C. arabica, and plant height and stem diameter showed S-curve with the day ordinal number, and leaf photosynthesis decreased significantly under drought stress but most photosynthesis index recovered somewhat after rewatering. Compared with I F-F , I L-F increased dry bean yield by 6.9%, while I M-F and I S-F decreased dry bean yield by 15.2% and 38.5%, respectively; I L-F and I M-F increased water use efficiency by 18.8% and 6.0%, respectively, while I S-F decreased water use efficiency by 12.1%; I L-F increased nitrogen partial productivity by 6.1%, while I M-F and I S-F decreased nitrogen partial productivity by 14.0% and 36.0%, respectively. Compared with N H , N M increased dry bean yield and water use efficiency by 20.9% and 19.3%, while N L decreased dry bean yield and water use efficiency by 42.4% and 41.9%, respectively; N M and N L increased nitrogen partial

  9. Epidemiology of allergic reactions to hymenoptera stings in Irish school children.

    LENUS (Irish Health Repository)

    Jennings, Aisling

    2010-12-01

    The aim of this was to study generate the first epidemiological data regarding the prevalence of hymenoptera allergy among school children in Ireland. Questionnaires, including six sting-specific questions (1), were distributed to the parents of primary school children aged 6-8 and 11-13, divided equally between rural and urban backgrounds. From 110 schools, 4112 questionnaires were returned. A total of 1544 (37.5%) children had been stung in their lifetime. Among the total, 5.8% of children stung experienced a large local reaction, 3.4% had a mild (cutaneous) systemic reaction (MSR) and 0.8% experienced a moderate\\/severe systemic reaction (SSR); these figures respectively represent 2.2%, 1.3% and 0.2% of the total study group. On logistic regression analysis, older children and rural children were at a higher risk of being stung (OR 1.7; 95% CI 1.4-2.; OR 1.6; 95% CI 1.4-1.8 respectively). Rural dwellers and asthma sufferers were more likely to experience an SSR (OR 4.3; 95% CI 1.4-13.5 and OR 2.8; 95% CI 1.8-4.3, respectively). Hymenoptera stings are more common in rural than urban dwelling Irish children. Asthma imparted a greater risk of SSR in this study population. Severe reactions are unusual overall, occurring in <1% of those stung, a lower prevalence than in Israeli teenagers but in keeping with other European reports relating to young children.

  10. Anaphylactic Reactions After Discontinuation of Hymenoptera Venom Immunotherapy: A Clonal Mast Cell Disorder Should Be Suspected.

    Science.gov (United States)

    Bonadonna, Patrizia; Zanotti, Roberta; Pagani, Mauro; Bonifacio, Massimiliano; Scaffidi, Luigi; Olivieri, Elisa; Franchini, Maurizio; Reccardini, Federico; Costantino, Maria Teresa; Roncallo, Chiara; Mauro, Marina; Boni, Elisa; Rizzini, Fabio Lodi; Bilò, Maria Beatrice; Marcarelli, Anna Rosaria; Passalacqua, Giovanni

    2017-12-16

    Up to 75% of patients with severe anaphylactic reactions after Hymenoptera sting are at risk of further severe reactions if re-stung. Venom immunotherapy (VIT) is highly effective in protecting individuals with ascertained Hymenoptera venom allergy (HVA) and previous severe reactions. After a 3- to 5-year VIT course, most patients remain protected after VIT discontinuation. Otherwise, a lifelong treatment should be considered in high-risk patients (eg, in mastocytosis). Several case reports evidenced that patients with mastocytosis and HVA, although protected during VIT, can re-experience severe and sometimes fatal reactions after VIT discontinuation. To evaluate whether patients who lost protection after VIT discontinuation may suffer from clonal mast cell disorders. The survey describes the characteristics of patients who received a full course of VIT for previous severe reactions and who experienced another severe reaction at re-sting after VIT discontinuation. Those with a Red Española de Mastocitosis score of 2 or more or a serum basal tryptase level of more than 25 ng/mL underwent a hematological workup (bone marrow biopsy, KIT mutation, expression of aberrant CD25) and/or skin biopsy. Nineteen patients (mean age, 56.3 years; 89.5% males) were evaluated. All of them had received at least 4 years of VIT and were protected. After VIT discontinuation they were re-stung and developed, in all but 1 case, severe anaphylactic reactions (12 with loss of consciousness, in the absence of urticaria/angioedema). Eighteen patients (94.7%) had a clonal mast cell disorder, 8 of them with normal tryptase. Looking at this selected population, we suggest that mastocytosis should be considered in patients developing severe reactions at re-sting after VIT discontinuation and, as a speculation, patients with mastocytosis and HVA should be VIT-treated lifelong. Copyright © 2017. Published by Elsevier Inc.

  11. Added sensitivity of component-resolved diagnosis in hymenoptera venom-allergic patients with elevated serum tryptase and/or mastocytosis

    DEFF Research Database (Denmark)

    Michel, J B; Brockow, K; Darsow, U

    2016-01-01

    BACKGROUND: Anaphylaxis caused by hymenoptera venom allergy is associated with elevation of baseline serum tryptase (sBT) and/or mastocytosis in about 5% of patients. Up to now, no information has become available on single venom allergen sIgE reactivity and the usefulness of component......-resolved approaches to diagnose this high-risk patient group. To address the component-resolved sIgE sensitization pattern and diagnostic sensitivity in hymenoptera venom-allergic patients with elevated sBT levels and/or mastocytosis, a panel of yellow jacket and honeybee venom allergens was applied on a widely used...... IgE immunoassay platform. METHODS: Fifty-three patients with mastocytosis and/or elevated sBT tryptase level and systemic reactions to hymenoptera venoms were analyzed for their IgE reactivity to recombinant yellow jacket and honeybee venom allergens by Immulite3 g. RESULTS: sIgE reactivity to Ves v...

  12. Comparative mitogenomics of Braconidae (Insecta: Hymenoptera) and the phylogenetic utility of mitochondrial genomes with special reference to Holometabolous insects

    Science.gov (United States)

    2010-01-01

    Background Animal mitochondrial genomes are potential models for molecular evolution and markers for phylogenetic and population studies. Previous research has shown interesting features in hymenopteran mitochondrial genomes. Here, we conducted a comparative study of mitochondrial genomes of the family Braconidae, one of the largest families of Hymenoptera, and assessed the utility of mitochondrial genomic data for phylogenetic inference at three different hierarchical levels, i.e., Braconidae, Hymenoptera, and Holometabola. Results Seven mitochondrial genomes from seven subfamilies of Braconidae were sequenced. Three of the four sequenced A+T-rich regions are shown to be inverted. Furthermore, all species showed reversal of strand asymmetry, suggesting that inversion of the A+T-rich region might be a synapomorphy of the Braconidae. Gene rearrangement events occurred in all braconid species, but gene rearrangement rates were not taxonomically correlated. Most rearranged genes were tRNAs, except those of Cotesia vestalis, in which 13 protein-coding genes and 14 tRNA genes changed positions or/and directions through three kinds of gene rearrangement events. Remote inversion is posited to be the result of two independent recombination events. Evolutionary rates were lower in species of the cyclostome group than those of noncyclostomes. Phylogenetic analyses based on complete mitochondrial genomes and secondary structure of rrnS supported a sister-group relationship between Aphidiinae and cyclostomes. Many well accepted relationships within Hymenoptera, such as paraphyly of Symphyta and Evaniomorpha, a sister-group relationship between Orussoidea and Apocrita, and monophyly of Proctotrupomorpha, Ichneumonoidea and Aculeata were robustly confirmed. New hypotheses, such as a sister-group relationship between Evanioidea and Aculeata, were generated. Among holometabolous insects, Hymenoptera was shown to be the sister to all other orders. Mecoptera was recovered as the

  13. Comparative mitogenomics of Braconidae (Insecta: Hymenoptera and the phylogenetic utility of mitochondrial genomes with special reference to Holometabolous insects

    Directory of Open Access Journals (Sweden)

    Shi Min

    2010-06-01

    Full Text Available Abstract Background Animal mitochondrial genomes are potential models for molecular evolution and markers for phylogenetic and population studies. Previous research has shown interesting features in hymenopteran mitochondrial genomes. Here, we conducted a comparative study of mitochondrial genomes of the family Braconidae, one of the largest families of Hymenoptera, and assessed the utility of mitochondrial genomic data for phylogenetic inference at three different hierarchical levels, i.e., Braconidae, Hymenoptera, and Holometabola. Results Seven mitochondrial genomes from seven subfamilies of Braconidae were sequenced. Three of the four sequenced A+T-rich regions are shown to be inverted. Furthermore, all species showed reversal of strand asymmetry, suggesting that inversion of the A+T-rich region might be a synapomorphy of the Braconidae. Gene rearrangement events occurred in all braconid species, but gene rearrangement rates were not taxonomically correlated. Most rearranged genes were tRNAs, except those of Cotesia vestalis, in which 13 protein-coding genes and 14 tRNA genes changed positions or/and directions through three kinds of gene rearrangement events. Remote inversion is posited to be the result of two independent recombination events. Evolutionary rates were lower in species of the cyclostome group than those of noncyclostomes. Phylogenetic analyses based on complete mitochondrial genomes and secondary structure of rrnS supported a sister-group relationship between Aphidiinae and cyclostomes. Many well accepted relationships within Hymenoptera, such as paraphyly of Symphyta and Evaniomorpha, a sister-group relationship between Orussoidea and Apocrita, and monophyly of Proctotrupomorpha, Ichneumonoidea and Aculeata were robustly confirmed. New hypotheses, such as a sister-group relationship between Evanioidea and Aculeata, were generated. Among holometabolous insects, Hymenoptera was shown to be the sister to all other orders

  14. Recuperação do cafeeiro (Coffea arabica L. após recepa, submetido a diferentes lâminas de água e parcelamentos da adubação = Recovery of coffee tree (Coffea arabica L. after pruning under different irrigation depths

    Directory of Open Access Journals (Sweden)

    Kelte Resende Arantes

    2009-04-01

    Full Text Available Visando reduzir o período de recuperação das lavouras após a recepa, neste trabalho objetivou-se avaliar a influência de diferentes lâminas de irrigação e do parcelamento da adubação sobre o crescimento do cafeeiro. Foram utilizadas quatro diferentes lâminas de irrigação (0, 40, 80 e 120% da ECA-Precipitação e três parcelamentos da adubação com N e K (quatro, oito e 12 vezes. O experimento foi conduzido no Sul de Minas Gerais, com o cafeeiro (Coffea arabica cv. Topázio MG-1190, plantado no espaçamento de 1,8 x 0,7 m e recepado 65 meses após o plantio. O delineamentoexperimental utilizado foi em blocos casualizados com parcelas subdivididas. Os parâmetros avaliados foram altura da planta, diâmetro do ramo ortotrópico, número de internódios e diâmetro da copa. O crescimento do cafeeiro foi influenciado pela irrigação, mas não foi influenciado pelo parcelamento da adubação. A lâmina de 120% do saldo do balanço ECA - P proporcionou maior crescimento.In order to reduce the recovery period of the orchard coffeeafter pruning, this work was carried out with the objective of evaluating the influence of different irrigation depths and splitting of N and K with fertirrigation on the growth of coffee plants. The experiment was carried out in southern Minas Gerais State with coffeaarabica cv. Topázio MG-1190 implanted with 1.8 x 0.7 m spacing. Irrigations corresponded to 0, 40, 80 and 120% of the evaporation from a Class A pan – precipitation (ECA-P and N and K split in 4, 8 and 12 applications. A randomized block design with four replicationswas used. Plant height, diameter of orthotropic branches, number of internodes and crown diameter were evaluated. The fertilizer splitting was found not to provide any increase in growth of the coffee plant. The depth of 120%*ECA-P provided the greatest growth ofcoffee plants when compared to others.

  15. Ichneumonidae (Hymenoptera parasitoids of Lepidoptera caterpillars feeding on Croton floribundus Spreng (Euphorbiaceae Ichneumonidae (Hymenoptera parasitóides de larvas de Lepidoptera associadas a Croton floribundus Spreng (Euphorbiaceae

    Directory of Open Access Journals (Sweden)

    Luciana Bueno dos Reis Fernandes

    2010-06-01

    Full Text Available Parasitoids of the family Ichneumonidae (Hymenoptera were obtained during an inventory of Lepidoptera larvae caught feeding in the wild on Croton floribundus (Euphorbiaceae. The Lepidoptera larvae were collected from host plants along trails inside three preserved forest areas in the Brazilian state of São Paulo. Fifteen different species of Ichneumonidae belonging to five subfamilies (Banchinae, Campopleginae, Cremastinae, Mesochorinae and Metopiinae were obtained. Seven species of Ichneumonidae were reared from leaf rollers: Meniscomorpha sp. (Banchinae and Leurus caeruliventris (Cresson (Metopiinae from Dichomeris sp. (Gelechiidae; Mesochorus sp.1 (Mesochorinae [as a parasitoid of Hypomicrogaster sp. (Braconidae, Microgastrinae], Campoplex sp. (Campopleginae and Leurus sp. from Olethreutinae sp. (Tortricidae; Sphelodon annulicornis Morley (Banchinae and Eutanygaster brevipennis Cameron (Cremastinae were also reared from two unidentified species of Gelechiidae. The other eight species were reared from the larvae of exposed feeders: Diradops sp. (Banchinae from Miselia albipuncta Hampson (Noctuidae, Casinaria sp. (Campopleginae from Hymenomima conia Prout (Geometridae, Charops sp. (Campopleginae from Bagisara paulensis Schaus (Noctuidae and Oxydia vesulia (Cramer (Geometridae, two species of Hyposoter Förster (Campopleginae from Semaeopus sp. (Geometridae and H. conia, two species of Microcharops Roman (Campopleginae from B. paulensis and an unidentified species of Limacodidae and Mesochorus sp. 2 [reared from what was probably Aleiodes sp. (Braconidae, Rogadinae] from an unidentified species of Noctuidae.Parasitóides da família Ichneumonidae (Hymenoptera foram obtidos durante um inventário de larvas de Lepidoptera sobre Croton floribundus (Euphorbiaceae. As larvas de Lepidoptera foram coletadas sobre as plantas que ocorrem nas bordas de caminhos em três áreas preservadas de mata do Estado de São Paulo, Brasil. Quinze esp

  16. Melhoramento do cafeeiro: XLII. Produtividade de progênies derivadas de hibridação dos cultivares Laurina e Mundo Novo Coffee breeding: XLII. Yield of progenies from crosses of Laurina and Mundo Novo cultivars of Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    Alcides Carvalho

    1988-01-01

    Full Text Available O cultivar Laurina de Coffea arabica L. caracteriza-se pelo pequeno porte, folhas de dimensões reduzidas, frutos afilados na base, sementes pequenas e afiladas, pequeno rendimento e reduzida produção. Apresenta, no entanto, bebida de boa qualidade e baixo teor de cafeína nas sementes. Suas principais características são controladas pela ação de um par de alelos recessivos lrlr, de acentuado efeito pleiotrópico. Devido ao atual interesse do comércio por produto de baixo teor de cafeína, iniciaram-se pesquisas tendo em vista principalmente aumentar a produtividade do 'Laurina'. Para esse fim, realizaram-se numerosas hibridações de cafeeiros do 'Laurina' com os do 'Mundo Novo' (Coffea arabica e, posteriormente, retrocruzamentos com o 'Mundo Novo'. Estudaram-se as progênies F2 e retrocruzamentos com o 'Mundo Novo' (RC em Campinas, em um experimento, anotando-se as produções por oito anos consecutivos. Separaram-se algumas progênies F2 em dois grupos, antes do plantio: normais (LrLr,Lrlr e laurina (Irlr. Como testemunhas, usaram-se progênies do 'Mundo Novo' e 'Catuaí Amarelo' de C. arabica. O conjunto de plantas F2 do grupo laurina e os retrocruzamentos tiveram produção média maior do que as plantas F2 normais, porém menor do que as testemunhas. Alguns retrocruzamentos e progênies F2 apresentaram plantas com razoável produtividade, indicando que, através de retrocruzamentos com o 'Mundo Novo', podem-se obter novos tipos comerciais com as características morfológicas do 'Laurina'. Fizeram-se considerações sobre a melhor capacidade de combinação do 'Laurina' com algumas seleções do 'Mundo Novo'.The Laurina cultivars of Coffea arabica L. has a reduced plant size, small leaves, small and pointed seeds and low yield capacity. However the seeds have a good cup quality and the desirable characteristic of low caffeine content The Laurina phenotype is supposed to be controlled by a pair of recessive alleles lrlr, with

  17. TUMBUHAN YANG DIKUNJUNGI LEBAH PEKERJA Apis (Hymenoptera: Apidae DI SUMATERA BARAT

    Directory of Open Access Journals (Sweden)

    Jasmi Jasmi

    2016-09-01

    Full Text Available Research about plant visited by Apis forager was conducted at altitude < 500 m and 600-1400 m of West Sumatra from December 2009-July 2010. The plant visited by forager was collected at radius 500 m from nests in fruits cropping, coffea and cinnamom plantation and forest edge. Honeybee from genus Apisvisit on plant consisted of four species, those are A. andreniformis, A. cerana, A. dorsata and A. koschevnikovi. Plant visited by forager Apis consisted of 61 species belong to 26 families. The number of 36 plant species found at lowland and highland, 20 species only at lowland and five species at highland. Most of plant species visited by Apis forager are Asteraceae and Leguminoceae. About 29 plant species visited by Apis were agriculture crop and 32 species of non agriculture. Apis andreniformis visited 29 plant species, A. cerana 56 species, A. dorsata 57 species and A. koschevnikovi one plant species. Cucumis sativus was visited by four bee species, Galiansoga farviflora was visited only by A. cerana. Five plant species (Rorippa indica, Cinnamomum burmanii, Nasturrium indicum, Rorippa indica and Eryngium foetidum were visited by only A. dorsata. Other three plants species (Mangifera indica, Cytrus aurantifoliaand Oryza sativa were visited by A. cerana and A. andreniformis, 31 plant species were visited by A. cerana and A. dorsata, 21 species were visited by A. andreniformis, A. cerana and A. dorsata. Keywords: lebah madu, Apis forager, food source plant, visit

  18. Crescimento, fotossíntese e composição mineral em genótipos de Coffea com potencial para utilização como porta-enxerto Growth, photosynthesis and mineral composition of Coffea germplasm with potential as rootstock

    Directory of Open Access Journals (Sweden)

    Eduardo Lauriano Alfonsi

    2005-01-01

    Full Text Available Foi estudado o desenvolvimento da parte aérea e das raízes, as trocas gasosas fotossintéticas e a composição mineral nos genótipos de Coffea, Apoatã IAC 2258 (C. canephora; Bangelan IAC col. 5 (C. congensis X C. canephora; Catuaí IAC 144 (C. arabica; Excelsa (C. liberica, var. dewevrei e Piatã (IAC 387 (C. arabica X C. liberica, var. dewevrei, visando conhecer seus potenciais para utilização como porta-enxerto em C. arabica. Utilizaram-se plantas jovens cultivadas em terra, contida em saco plástico e em tubos de PVC sob telado, e no campo em plantas de quatro anos de idade. Em 'Bangelan' observaram-se maior comprimento da raiz pivotante e mais quantidade de raízes secundárias do que em 'Catuaí' e 'Piatã'. Os teores foliares de potássio do 'Piatã', 'Apoatã' e 'Bangelan' foram maiores aos observados para 'Catuaí' e 'Excelsa'. No campo, com baixa disponibilidade de água e nutrientes, os teores foliares de fósforo em 'Apoatã' e 'Piatã' foram maiores que em 'Catuaí' e 'Excelsa'. Em 'Catuaí,' notou-se maior eficiência na absorção de todos os micronutrientes (B, Cu, Mn e Zn, exceto ferro. A taxa fotossintética, condutância estomática e transpiração decresceram à tarde em todos os genótipos. Sob défice hídrico no solo, houve quedas significativas nas taxas fotossintéticas, condutância estomática e transpiração, sendo mais pronunciadas em 'Apoatã' e 'Excelsa', e menos em 'Catuaí' e 'Piatã', em relação aos valores observados sem restrição hídrica. A taxa fotossintética do 'Apoatã' foi menos influenciada pelo teor de água no solo em relação aos demais genótipos, que mostraram quedas acentuadas no período da tarde. Verificaram-se em 'Bangelan' e 'Apoatã' características favoráveis para a utilização como porta-enxerto em C. arabica.The development of shoot and roots, photosynthetic gas exchange and mineral composition of Coffea plants were studied in 'Apoatã' IAC 2258 (C. canephora, 'Bangelan

  19. Effect of Parasitoid: Host Ratio and Parasitoid and Host Group Size on Fitness of Spathius galinae (Hymenoptera: Braconidae), a Parasitoid of Emerald Ash Borer (Coleoptera: Buprestidae): Implications for Mass-Rearing

    Science.gov (United States)

    Producing insect natural enemies in laboratories or insectaries for biological pest control is often expensive, and developing cost-effective rearing techniques is a goal of many biological control programs. Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a recently described...

  20. Two genera Foersteria Szépligeti, 1896 and Polydegmon Foerster, 1862 (Hymenoptera, Braconidae, Brachistinae) from China, with description of a new species.

    Science.gov (United States)

    Yan, Cheng-Jin; He, Jun-Hua; Chen, Xue-Xin

    2013-01-01

    The genera Foersteria Szépligeti, 1896 and Polydegmon Foerster, 1862 (Hymenoptera, Braconidae, Brachistinae) are recorded for the first time from China. A new species, Foersteria xinjiangensis Yan & Chen, sp. nov., is described and illustrated. A key to the Palaearctic species of Foersteria is given. In addition, Polydegmon sinuatus Foerster, 1862 is illustrated in detail for the first time.

  1. A new species of Klabonosa Bouček (Hymenoptera: Pteromalidae) reared from eggs of Endochus sp. (Hemiptera: Reduviidae) from India.

    Science.gov (United States)

    Gupta, Ankita; Yeshwanth, H M; Sureshan, P M

    2018-04-23

    The genus Klabonosa Bouček (Hymenoptera: Pteromalidae) is recorded for the first time from the Oriental region, with both sexes of K. indica Gupta, Sureshan Yeshwanth sp. n. reared from eggs of the assassin bug Endochus sp. (Hemiptera: Reduviidae) on Artocarpus heterophyllus Lam. (Moraceae). The male is formally described and illustrated for the first time for the genus.

  2. Primera cita de Aleiodes laphygmae (Hymenoptera: Braconidae para Argentina y de su asociación con larvas de Spodoptera eridania (Lepidoptera: Noctuidae

    Directory of Open Access Journals (Sweden)

    Liliana VALVERDE

    2012-01-01

    Full Text Available Se reporta por primera vez para Argentina Aleiodes laphygmae (Viereck (Hymenoptera: Braconidae y como parasitoide de larvas de Spodoptera eridania (Stoll (Lepidoptera: Noctuidae, en plantaciones de soja en la provincia de Tucumán (Argentina. Se provee información biológica como hábitos, hospedadores y distribución.

  3. Structural Examination of the Dufour's Gland of the Cavity-nesting Bees Osmia lignaria Say and Megachile rotundata (Fabricius) (Hymenoptera: Megachilidae)

    Science.gov (United States)

    The Dufour’s gland of two solitary cavity-nesting bees, Osmia lignaria and Megachile rotundata (Hymenoptera: Megachilidae), were examined with microscopy to determine the structure and arrangement of the gland as part of the sting apparatus. The Dufour’s glands of these two bee species are similar ...

  4. Test of nonhost angiosperm volatiles and verbenone to protect trap trees for Sirex noctilio (Hymenoptera: Siricidae) from attacks by bark beetles (Coleoptera: Scolytidae) in the Northeastern United States

    Science.gov (United States)

    Kevin Dodds; Daniel Miller

    2010-01-01

    Sirex noctilio F. (Hymenoptera: Siricidae) is an invasive woodwasp, currently established in northeastern North America. In other regions of the world, stressed trap trees are used to monitor populations of S. noctilio and to provide inoculation points for the biological control nematode Deladenus siricidicola Bedding. However, the operational use of trap trees for S....

  5. Primera cita de Aleiodes laphygmae (Hymenoptera: Braconidae) para Argentina y de su asociación con larvas de Spodoptera eridania (Lepidoptera: Noctuidae)

    OpenAIRE

    Liliana VALVERDE; D. Carolina BERTA; Marcelo GERONIMO GOMEZ

    2012-01-01

    Se reporta por primera vez para Argentina Aleiodes laphygmae (Viereck) (Hymenoptera: Braconidae) y como parasitoide de larvas de Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae), en plantaciones de soja en la provincia de Tucumán (Argentina). Se provee información biológica como hábitos, hospedadores y distribución.

  6. First record of Platygaster luteipes Buhl (Hymenoptera Platygastridae) from leaf galls on black pepper along with first report of the species from India

    DEFF Research Database (Denmark)

    Anjana, M.; Rajmohana, K.; Buhl, Peter Neerup

    2016-01-01

    Platygaster luteipes Buhl (Hymenoptera: Platygastridae) is reported for the first time from India. Several specimens of both sexes of the species were reared from the globular galls on mature leaves of black pepper. The hitherto undescribed male of the species is characterized and illustrated....

  7. First discovery of the family Tanaostigmatidae (Hymenoptera: Chalcidoidea) from China with a description of a new gall-making species utilizing kudzu leaves

    Science.gov (United States)

    Yang Zhong-qi; Sun Jiang-hua; James P. Pitts

    2004-01-01

    A new species of Tanaostigmodes (Hymenoptera: Chalcidoidea, Tanaostigmatidae) is described from China- Tanaostigmodes puerariae sp. nov. This is the first record of this family in China. This new species has potential as a biological control agent for control of kudzu, Pueraria lobate, in the U.S., because its...

  8. Comparison of the olfactory preferences of four species of filth fly pupal parasitoid species (Hymenoptera: Pteromalidae) for hosts in equine and bovine manure

    Science.gov (United States)

    House flies (Musca domestica L.) and stable flies (Stomoxys calcitrans (L.)) (Diptera: Muscidae) are common pests in equine and cattle facilities. Pupal parasitoids primarily in the genera Spalangia and Muscidifurax (Hymenoptera: Pteromalidae) can be purchased for biological control of these flies. ...

  9. Evidence for divergence in cuticular hydrocarbon sex pheromone between California and Mississippi (United States of America) populations of bark beetle parasitoid Roptrocerus xylophagorum (Hymenoptera: Pteromalidae).

    Science.gov (United States)

    Brian Sullivan; Nadir Erbilgin

    2014-01-01

    Roptrocerus xylophagorum (Ratzeburg) (Hymenoptera: Pteromalidae) is a common Holarctic parasitoid of the larvae and pupae of bark beetles (Coleoptera: Curculionidae: Scotytinae). In no-choice laboratory bioassays, we found that male wasps derived either from northern California or southwestern Mississippi, United States of America more frequently displayed sexual...

  10. A New Species of Megastigmus Dalman (Hymenoptera: Torymidae) Reared from Seeds of Atlantic White Cedar (Cupressaceae), with Notes on Infestation Rates

    Science.gov (United States)

    J.J. Turgeon; K. Kamijo; G. DeBarr

    1997-01-01

    A new species, Megastigmus thyoides Kamijo (Hymenoptera: Torymidae), which emerged from seeds of Atlantic white cedar, Chamaecyparis thyoides (L.) B.S.P., collected in eastern United States is described and illustrated. This is the first record of this genus exploiting seeds of Cupressaceae in the Nearctic region. An average of 7% of the seeds collected from five sites...

  11. New record and re-description of a gall-forming aphid (Hemiptera: Aphididae, commonly confused in the north of South America, associated with an ant (Hymenoptera: Formicidae

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    Ronald Simbaqueba-Cortés

    2015-04-01

    Full Text Available The gall-forming aphid Tetraneura fusiformis is recorded for the first time for Northern South America. Its identity is clarified, and descriptions of this species and that of T. nigriabdominalis, with which it is commonly confused, are offered. The association of this sap sucking insect with the ant Linepithema angulatum (Hymenoptera: Formicidae is recorded for the first time as well

  12. The role of selected soil fauna as predators of Apethymus abdominalis Lep. (Hymenoptera: Tenthredinidae) in oak forests in the District Caiuti, Romania

    Science.gov (United States)

    C. Ciornei; N. Popa; L. Ciuca; C. Rang

    2003-01-01

    The present study was initiated in 2001 in the oak forests from Trotus valley (Forest District Caiucti - Bacau, Romania) which were heavily infested by oak sawflies Apethymus abdominalis Lep. (Hymenoptera: Tenthredinidae), in order to understand better the role of soil-inhabitating predators in population regulation of this pest.

  13. Review of the genus Craspedolcus Enderlein sensu lato in China, with the description of a new genus and four new species (Hymenoptera, Braconidae, Braconinae)

    NARCIS (Netherlands)

    Li, Y.; Achterberg, van C.; Chen, X.-x.

    2017-01-01

    A new genus is split off the genus Craspedolcus Enderlein, 1920 (Hymenoptera, Braconidae, Braconinae): Maculibracon gen. n. with type species Maculibracon abruptus sp. n. The genus Craspedolcus Enderlein sensu stricto is redefined, a key to both genera and to their species in China, Thailand and

  14. Additions to the fauna of Braconidae from Madeira and Selvagens Islands, with the description of five new species (Hymenoptera: Braconidae: Homolobinae, Alysiinae, Opiinae)

    NARCIS (Netherlands)

    Achterberg, van C.; Aguiar, Franquinho A.M.

    2009-01-01

    Twenty-one species of the family Braconidae (Hymenoptera) are added to the checklist of Braconidae from Madeira, resulting in 113 species, of which 17 species are endemic to Madeira Islands and 4 species are only known from Madeira and Canary Islands. Five species are reported new for the Selvagens

  15. The Behavioural Abilities of Meteorus versicolor Wesm. and Rogas rossicus Kok. (Hymenoptera, Branconidae) Parasitoids of Tephrina arenacearia Den. Et Schiff. (Lepidoptera, Geometridae)

    OpenAIRE

    KERIMOVA, Ilhama Gudrat Gizi

    1998-01-01

    This paper concerns with the behavioural abilities of Meteorus versicolor Wesm. and Rogas rossicus Kok. (Hymenoptera, Braconidae)-internal parasitoids of Thephrina arenacearia Den. et Schiff. (Lepidoptera, Geometridae) as well. Periods of development phases and searching, ovipositional and mating behaviour of both parasitoids have been investigated.

  16. Effects of venom immunotherapy on serum level of CCL5/RANTES in patients with Hymenoptera venom allergy.

    Science.gov (United States)

    Gawlik, Radoslaw; Glück, Joanna; Jawor, Barbara; Rogala, Barbara

    2015-01-01

    Hymenoptera venoms are known to cause life-threatening IgE-mediated anaphylactic reactions in allergic individuals. Venom immunotherapy is a recommended treatment of insect allergy with still the mechanism not being completely understood. We decided to assess the serum CCL5/RANTES level in patients who experienced severe anaphylactic reaction to Hymenoptera venom and to find out changes in the course of immunotherapy. Twenty patients (9 men, 11 women, mean age: 31.91 ± 7.63 years) with history of anaphylactic reaction after insect sting were included into the study. Diagnosis was made according to sIgE and skin tests. All of them were enrolled into rush venom immunotherapy with bee or wasp venom extracts (Pharmalgen, ALK-Abello, Horsholm, Denmark). Serum levels of CCL5/RANTES were measured using a commercially available ELISA kit (R&D Systems, Minneapolis, MN). CCL5/RANTES serum concentration are higher in insect venom allergic patients than in healthy controls (887.5 ± 322.77 versus 387.27 ± 85.11 pg/ml). Serum concentration of CCL5/RANTES in insect venom allergic patient was significantly reduced in the course of allergen immunotherapy already after 6 days of vaccination (887.5 ± 322.77 versus 567.32 ± 92.16 pg/ml). CCL5/RANTES serum doesn't correlate with specific IgE. Chemokine CCL5/RANTES participates in allergic inflammation induced by Hymenoptera venom allergens. Specific immunotherapy reduces chemokine CCL5/RANTES serum level already after initial days of venom immunotherapy.

  17. A Total-Evidence Approach to Dating with Fossils, Applied to the Early Radiation of the Hymenoptera

    Science.gov (United States)

    Ronquist, Fredrik; Klopfstein, Seraina; Vilhelmsen, Lars; Schulmeister, Susanne; Murray, Debra L.; Rasnitsyn, Alexandr P.

    2012-01-01

    Abstract Phylogenies are usually dated by calibrating interior nodes against the fossil record. This relies on indirect methods that, in the worst case, misrepresent the fossil information. Here, we contrast such node dating with an approach that includes fossils along with the extant taxa in a Bayesian total-evidence analysis. As a test case, we focus on the early radiation of the Hymenoptera, mostly documented by poorly preserved impression fossils that are difficult to place phylogenetically. Specifically, we compare node dating using nine calibration points derived from the fossil record with total-evidence dating based on 343 morphological characters scored for 45 fossil (4--20 complete) and 68 extant taxa. In both cases we use molecular data from seven markers (∼5 kb) for the extant taxa. Because it is difficult to model speciation, extinction, sampling, and fossil preservation realistically, we develop a simple uniform prior for clock trees with fossils, and we use relaxed clock models to accommodate rate variation across the tree. Despite considerable uncertainty in the placement of most fossils, we find that they contribute significantly to the estimation of divergence times in the total-evidence analysis. In particular, the posterior distributions on divergence times are less sensitive to prior assumptions and tend to be more precise than in node dating. The total-evidence analysis also shows that four of the seven Hymenoptera calibration points used in node dating are likely to be based on erroneous or doubtful assumptions about the fossil placement. With respect to the early radiation of Hymenoptera, our results suggest that the crown group dates back to the Carboniferous, ∼309 Ma (95% interval: 291--347 Ma), and diversified into major extant lineages much earlier than previously thought, well before the Triassic. [Bayesian inference; fossil dating; morphological evolution; relaxed clock; statistical phylogenetics.] PMID:22723471

  18. Temporal Activity Patterns of the Spider Wasp Pepsis montezuma Smith (Hymenoptera: Pompilidae in a Disturbed Lower Montane Rainforest (Manizales, Colombia

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    Carlos Restrepo-Giraldo

    2012-01-01

    Full Text Available We studied the temporal activity pattern of the spider wasp Pepsis montezuma Smith (Hymenoptera: Pompilidae in a disturbed lower montane rainforest, which is located in the city of Manizales, Colombia, at an altitude of 2,150 m. Females of this species are diurnal with two peaks of activity: one in the morning and the other in the afternoon. During the morning, nectar foraging occurred at Baccharis latifolia. During the afternoon, females hunted for tarantulas of the genus Pamphobeteus (Araneae: Theraphosidae, which were dragged backwards to the nest by the wasp. The nest was excavated before hunting. This is the first description of the behavior of Pepsis montezuma.

  19. Three new species of Horismenus Walker (Hymenoptera: Eulophidae) associated with seed pods of Pithecellobium dulce (Fabaceae).

    Science.gov (United States)

    Pikart, Tiago G; Costa, Valmir A; Hansson, Christer; Zanuncio, José C; Serrão, José E

    2015-08-04

    Horismenus abnormicaulis sp. nov., H. patensis sp. nov. and H. zuleidae sp. nov. (Hymenoptera: Eulophidae), all authored by Pikart, Costa & Hansson, are described from material obtained from seed pods of Pithecellobium dulce (Roxb.) Benth. (Fabaceae) collected in Northeastern Brazil. The seed pods were infested with larvae of Coleoptera (Chrysomelidae (Bruchinae) and Curculionidae). The associations of the Horismenus species and the beetle larvae have not been established. Morphological similarities between these new species and previously described species with host known suggest that H. patensis and H. zuleidae are primary parasitoids of Bruchinae, whereas H. abnormicaulis may act as a hyperparasitoid on other Horismenus species. The three species are compared with similar species of Horismenus.

  20. Notes on Neotropical Eumeninae, with the description of a new species of Pachodynerus de Saussure (Hymenoptera, Vespidae

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    Marcel Gustavo Hermes

    2013-06-01

    Full Text Available Notes on Neotropical Eumeninae, with the description of a new species of Pachodynerus de Saussure (Hymenoptera, Vespidae. Taxonomic information on Neotropical Eumeninae is provided. A new species, Pachodynerus fessatus sp. nov. is described from southeastern São Paulo, Brazil. Additional material of Pachodynerus sericeus (Fox was examined, representing the first further specimens after the original description and including the previously unknown male. The examination of new material of the genus Stenonartonia adds some new distribution records and shows some previously unrecorded individual variation for some species. The males of Stenonartonia guaraya Garcete-Barrett and Stenonartonia rejectoides Garcete-Barrett are described for the first time.

  1. Fauna de Campopleginae (Hymenoptera, Ichneumonidae) em hortas orgânicas em Araraquara e São Carlos, SP, Brasil

    OpenAIRE

    Sandonato, Diogo Loibel; Onody, Helena Carolina; Penteado-Dias, Angélica Maria

    2010-01-01

    Fauna de Campopleginae (Hymenoptera, Ichneumonidae) em hortas orgânicas em Araraquara e São Carlos, SP, Brasil. De março de 2006 a fevereiro 2007 foram realizadas doze amostragens utilizando armadilhas Malaise em cada uma das três áreas de cultivos orgânicos estudadas. Um total de 1773 espécimes foram identificados em dez gêneros de Campopleginae: Campoctonus Viereck, 1912, Campoletis Föster, 1869, Casinaria Holmgren, 1859, Charops Holmgren, 1859, Cryptophion Viereck, 1913, Diadegma Föster, 1...

  2. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) in Brazil.

    Science.gov (United States)

    Marsaro Júnior, A L; Peronti, A L B G; Costa, V A; Morais, E G F; Pereira, P R V S

    2016-02-01

    Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam.) de Wit (Fabaceae), Morus nigra L. (Moraceae), Citrus reticulata Blanco (Rutaceae), Tectona grandis L. f. (Verbenaceae), Anacardium occidentale (Anacardiaceae), Annona squamosa L. and Xylopia aromatica (Lam.) Mart. (Annonaceae), in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  3. Capalictus, a new subgenus of Lasioglossum Curtis 1833 from South Africa, with description of three new species (Hymenoptera, Apoidea, Halictidae

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    Jason Gibbs

    2012-11-01

    Full Text Available Capalictus, a new subgenus of Lasioglossum Curtis, 1833 (Hymenoptera, Apoidea, Halic-tidae, endemic to the South African Cape Province, is described. The type species is Halictus mosselinus Cockerell, 1945. Evylaeus (Sellalictus fynbosensis (Pauly et al., 2008 is a new junior synonym of L. (C. mosselinum. Three new species are described: Lasioglossum (Capalictus hantamense sp. nov., L. (C. tigrinum sp. nov. and L. (C. timmermanni sp. nov. DNA sequence data from three nuclear genes support morphologically-determined species limits. Capalictus is a basal clade of the Hemihalictus series of Lasioglossum.

  4. Type specimens of Hymenoptera deposited in the Museu de Zoologia da Universidade de São Paulo, Brazil (excluding Aculeata

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    Helena C. Onody

    2014-01-01

    Full Text Available The present paper lists the type specimens of Hymenoptera, excluding Aculeata, deposited in the Museu de Zoologia da Universidade de São Paulo, Brazil. We record all labels contents and also additional information from MZSP registers, published material, and other available sources. High resolution photographs of holotypes, lectotypes and syntypes are available through links to Specimage - the image database of The Ohio State University, where they are archived. The collection comprises a total of 332 type-specimens (32 holotypes, 266 paratypes, 12 syntypes, 20 paralectotypes and two lectotypes of eight superfamilies, 18 families, 31 subfamilies, 43 genera and 83 species.

  5. On the nesting biology of Pirhosigma Giordani Soika (Hymenoptera, Vespidae, Eumeninae, with special reference to the use of vegetable matter

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    Marcel G. Hermes

    2013-12-01

    Full Text Available On the nesting biology of Pirhosigma Giordani Soika (Hymenoptera, Vespidae, Eumeninae, with special reference to the use of vegetable matter. The use of vegetable matter in nest building is not widespread among the Eumeninae, and is reported for the first time for the two species of potter wasps Pirhosigma superficiale and P. limpidum. These wasps make mostly spherical mud nests over which they attach small pieces of unmasticated plant matter. Use of plant fragments in this group of wasps is interpreted as camouflage behavior.

  6. A new Anagyrus (Hymenoptera: Encyrtidae) from Argentina, parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis (Cactaceae).

    Science.gov (United States)

    Triapitsyn, Serguei V; Aguirre, María B; Logarzo, Guillermo A

    2016-05-26

    A new species of Anagyrus Howard (Hymenoptera: Encyrtidae), A. lapachosus sp. n., is described from Salta Province of Argentina as a parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis cactus (Cactaceae). It is a candidate "new association" biological control agent for quarantine evaluation and possible following introduction to Puerto Rico (USA) against another Hypogeococcus sp., commonly called the Harrisia cactus mealybug and often misidentified as H. pungens Granara de Willink (according to our unpublished data the latter attacks only Amaranthaceae), which devastates or threatens the native cacti there and also in some other Caribbean islands (Triapitsyn, Aguirre et al. 2014; Carrera-Martínez et al. 2015).

  7. Primera cita de Aleiodes laphygmae (Hymenoptera: Braconidae para Argentina y de su asociación con larvas de Spodoptera eridania (Lepidoptera: Noctuidae First record of Aleiodes laphygmae (Hymenoptera: Braconidae for Argentina and its association with larvae of Spodoptera eridania (Lepidoptera: Noctuidae

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    Liliana Valverde

    2012-06-01

    Full Text Available Se reporta por primera vez para Argentina Aleiodes laphygmae (Viereck (Hymenoptera: Braconidae y como parasitoide de larvas de Spodoptera eridania (Stoll (Lepidoptera: Noctuidae, en plantaciones de soja en la provincia de Tucumán (Argentina. Se provee información biológica como hábitos, hospedadores y distribución.Aleiodes laphygmae (Viereck (Hymenoptera: Braconidae, is reported for the first time for Argentina. It is also reported parasitizing larvae of Spodoptera eridania (Stoll (Lepidoptera: Noctuidae in soybeans crops in Tucumán province (Argentina. Biological information on habits, hosts and distribution is provided.

  8. Preference by Vespula germanica (Hymenoptera: Vespidae) for processed meats: implications for toxic baiting.

    Science.gov (United States)

    Wood, G M; Hopkins, D C; Schellhorn, N A

    2006-04-01

    The German yellowjacket, Vespula germanica (F.) (Hymenoptera: Vespidae), was introduced into Australia in 1959 and has established throughout southern Australia. In urban environments, V. germanica is frequently a nuisance pest at public gatherings and to homeowners. In native environments, it has the potential to pose a threat to native invertebrates. The current practice for controlling the wasps is nest destruction with pesticide. However, locating the nest(s) is not always practical or possible. Meat baits impregnated with an insecticide that foraging wasps cut and carry back to the nest offer a means of suppressing wasps where the nest sites are unknown. The success of meat baits depends on the attractiveness and acceptance of the meat to the wasp and the mode of action of the insecticide. Our objective was to determine wasp preference and acceptance of five processed meats: canned chicken or fish and freeze-dried chicken, fish, or kangaroo. We found that more wasps visited and took freeze-dried kangaroo and canned chicken than the other baits. Canned and freeze-dried fish were similarly preferred, and freeze-dried chicken was the least attractive and accepted by foraging wasps. Our findings demonstrate that wasps prefer some processed meats and hence take more loads back to the nest. By combining a suitable insecticide with a meat bait preferred by wasps, the likelihood of effective suppression of nuisance wasp populations should be increased.

  9. Single-locus complementary sex determination in the inbreeding wasp Euodynerus foraminatus Saussure (Hymenoptera: Vespidae).

    Science.gov (United States)

    Stahlhut, J K; Cowan, D P

    2004-03-01

    The Hymenoptera have arrhenotokous haplodiploidy in which males normally develop from unfertilized eggs and are haploid, while females develop from fertilized eggs and are diploid. Multiple sex determination systems are known to underlie haplodiploidy, and the best understood is single-locus complementary sex determination (sl-CSD) in which sex is determined at a single polymorphic locus. Individuals heterozygous at the sex locus develop as females; individuals that are hemizygous (haploid) or homozygous (diploid) at the sex locus develop as males. sl-CSD can be detected with inbreeding experiments that produce diploid males in predictable proportions as well as sex ratio shifts due to diploid male production. This sex determination system is considered incompatible with inbreeding because the ensuing increase in homozygosity increases the production of diploid males that are inviable or infertile, imposing a high cost on matings between close relatives. However, in the solitary hunting wasp Euodynerus foraminatus, a species suspected of having sl-CSD, inbreeding may be common due to a high incidence of sibling matings at natal nests. In laboratory crosses with E. foraminatus, we find that sex ratios and diploid male production (detected as microsatellite heterozygosity) are consistent with sl-CSD, but not with other sex determination systems. This is the first documented example of sl-CSD in a hymenopteran with an apparent natural history of inbreeding, and thus presents a paradox for our understanding of hymenopteran genetics.

  10. Lack of behavioural evidence for kin avoidance in mate choice in a hymenopteran parasitoid (Hymenoptera: Braconidae).

    Science.gov (United States)

    Bourdais, D; Hance, T

    2009-05-01

    Mechanisms for inbreeding avoidance should be prevalent in insects that reproduce by arrhenotokous haplodiploidy because of the higher potential production of unviable diploid males in inbred matings. Few studies have focused on mating strategies in insect parasitoids and even less on kinship relationships during mate choice. In this study we tested avoidance of kin as mate in the parasitic wasp Aphidius matricariae (Hymenoptera: Braconidae) using an ethological approach. Key mating parameters, such as male wing fanning, latent period before genitalia contact and duration of copulation were measured. No evidence for kin avoidance in mate choice in both A. matricariae males and females was observed in our behaviour (no choice or choice tests) tests. This lack of ethological sib mating avoidance could be due to different factors such as sex determination rule different than the single locus complementary sex determination, making lower the proportion of diploid males in case of sib matings and thus its negative consequence. The existence of other inbreeding avoidance strategies and mechanisms that reduce the probability of 2 receptive relatives meeting in nature may be common, for example, inbred mating may be rare through differential dispersal, delayed maturation, or protandry.

  11. The type specimens of sawflies (Hymenoptera: Symphyta) of the Museo Nacional de Ciencias Naturales, Madrid.

    Science.gov (United States)

    Taeger, Andreas; París, Mercedes; Nieves-Aldrey, Jose Luis

    2014-04-16

    The type specimens of sawflies (Hymenoptera: Symphyta) housed in the Museo Nacional de Ciencias Naturales, Madrid, were examined. Lectotypes are designated and illustrated for the following 32 nominal taxa (preserved in the MNCN collection if not stated otherwise): Tenthredo acutiscutis Konow, 1908; Tenthredo aericeps Konow, 1907; Allantus albipectus Konow, 1907; Athalia bolivari Dusmet, 1896; Tristactus punctatus var. candidatus Konow, 1899; Tenthredo capistrata Konow, 1907; Megalodontes capitalatus Konow 1904 (coll. SDEI); Tenthredo casta Konow, 1908; Clydostomus cestatus Konow, 1908; Miocephala chalybea Konow, 1907 (coll. SDEI); Peus cupreiceps Konow, 1907; Metallopeus cupreolus Malaise, 1945 (coll. NHRS); Allantus dusmeti Konow, 1894 (coll. SDEI); Megalodontes dusmeti Enslin, 1914 (coll. ZSM); Megalodontes escalerai Konow, 1899; Tenthredo flavitarsis Konow, 1908; Sciopteryx galerita Konow, 1907; Tenthredo habenata Konow, 1907; Allantus inguinalis Konow, 1908; Clydostomus merceti Konow, 1908; Megalodontes merceti Konow 1904 (coll. SDEI); Tenthredo mordax Konow, 1908; Megalodontes mundus Konow, 1904; Tenthredo nimbata Konow, 1906; Tenthredo oculissima Konow, 1907; Peus pannulosus Konow, 1907; Tenthredo podagrica Konow, 1907; Arge segmentaria var. rufiventris Konow, 1899; Tenthredo rugiceps Konow, 1908; Tenthredo segrega Konow, 1908; Peus splendidus Konow 1907; Tenthredo suta Konow, 1906. Peus cupreiceps Konow, 1907, is considered to be a valid species. New synonymy is proposed for Tenthredo (Metallopeus) cupreiceps (Konow, 1907), comb. nov., spec. rev. (=Metallopeus cupreolus Malaise, 1945, syn. nov.; =Metallopeus inermis Malaise, 1945, syn. nov.). 

  12. Diurnal flight behavior of Ichneumonoidea (Insecta: Hymenoptera) related to environmental factors in a tropical dry forest.

    Science.gov (United States)

    González-Moreno, A; Bordera, S; Leirana-Alcocer, J; Delfín-González, H

    2012-06-01

    The biology and behavior of insects are strongly influenced by environmental conditions such as temperature and precipitation. Because some of these factors present a within day variation, they may be causing variations on insect diurnal flight activity, but scant information exists on the issue. The aim of this work was to describe the patterns on diurnal variation of the abundance of Ichneumonoidea and their relation with relative humidity, temperature, light intensity, and wind speed. The study site was a tropical dry forest at Ría Lagartos Biosphere Reserve, Mexico; where correlations between environmental factors (relative humidity, temperature, light, and wind speed) and abundance of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonoidea) were estimated. The best regression model for explaining abundance variation was selected using the second order Akaike Information Criterion. The optimum values of temperature, humidity, and light for flight activity of both families were also estimated. Ichneumonid and braconid abundances were significantly correlated to relative humidity, temperature, and light intensity; ichneumonid also showed significant correlations to wind speed. The second order Akaike Information Criterion suggests that in tropical dry conditions, relative humidity is more important that temperature for Ichneumonoidea diurnal activity. Ichneumonid wasps selected toward intermediate values of relative humidity, temperature and the lowest wind speeds; while Braconidae selected for low values of relative humidity. For light intensity, braconids presented a positive selection for moderately high values.

  13. Diversity of Hymenoptera (Insecta) on different ages of oil palm in Lekir Plantation, Perak

    Science.gov (United States)

    Azhari, Muhammad Luqman Hakim; Hazmi, Izfa Riza

    2018-04-01

    This study was conducted to determine the diversity of Hymenoptera on the different ages of oil palm namely plot 12, 9 and 5 years old. Sampling was carried out from November 2015 to February 2016 at Ladang Lekir, Perak using Malaise traps and Window trap. A total of 3052 individuals Hymenopteran consisting of 58 morphospecies and 35 subfamilies of 17 families were successfully collected. The most abundant species recorded was the Pimplinae.sp4 with 447 individuals (relative abundance, RA=14.51%). According to the plot, plot 9 years old have the highest reading for all three Peilou Equality Index (E'=0.983), Shannon Diversity Index (H'=3.939) and Simpson Diversity Index (D'=0.9795) with 55 species have been recorded. The t-tests showed that there were no significant difference in term of the diversity index (H') between palm plots 9 years old and 12 years old, while there were significant differences between the two plot (9 years old and 12 years old) with 5 old palm plot. The species accumulation curve showed that only 5 old palm plot nearly asymptotic. This study is expected to help the management to provide basic information for future research and as well, to develop and implement tools, methods, strategies in farm management practices of the oil palm plantations in Malaysia.

  14. SEARCHING AND PARASITISM OF Diatraea saccharalis (LEPIDOPTERA: CRAMBIDAE BY Trichospilus diatraeae (HYMENOPTERA: EULOPHIDAE

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    Elizangela Leite Vargas

    2013-05-01

    La capacidad de Trichospilus diatraeae Cherian y Margabandhu, 1942 (Hymenoptera: Eulophidae para buscar y parasitar las pupas de Diatraea saccharalis (Fabricius, 1794 (Lepidoptera: Crambidae en los tallos de la caña de azúcar fue estudiada. Para analizar la habilidad de búsqueda y parasitismo fueron utilizados tallos de la caña de azúcar (20 cm donde se introdujo una pupa de D. saccharalis (T1; pupas y orugas (T2 o pupa y residuos fecales (T3. Cada tallo fue colocado en una botella plástica transparente con 21 hembras de T. diatraeae. Esas pupas fueron individualizadas, luego de 72 h, en tubos de vidrio a 25 ± 1°C, 70 ± 10 % UR y 14 h de foto período. El experimento se desarrolló en un diseño completamente al azar, con tres tratamientos y 12 repeticiones. Los porcentajes de pupas de D. saccharalis parasitados por T. diatraeae fueron del 50,00 %, 83,33 % y 16,66 % en el T1, T2 y T3, respectivamente (c2 = 3.896, p = 0,04. La presencia de D. saccharalis en los tallos de caña de azúcar favorecieron la búsqueda y parasitismo de su hospedero.

  15. Activity of bifenthrin, chlorfenapyr, fipronil, and thiamethoxam against Argentine ants (Hymenoptera: Formicidae).

    Science.gov (United States)

    Wiltz, B A; Suiter, D R; Gardner, W A

    2009-12-01

    Bifenthrin, chlorfenapyr, fipronil, and thiamethoxam were evaluated for activity against the Argentine ant, Linepithema humile (Mayr) (Hymenoptera: Formicidae). Mobility impairment and lethal times were determined after topical treatments. Ants were immobilized most quickly by bifenthrin, followed by chlorfenapyr and thiamethoxam. After 2 h, the number of fipronil-treated ants unable to walk out of test arenas did not differ from control ants. Median lethal time (LT50) after topical treatment was lowest in the bifenthrin treatment, followed by thiamethoxam, chlorfenapyr, and then fipronil. Mortality due to horizontal exposure was evaluated at 10, 20, or 30 degrees C, with topically treated ant corpses serving as donors. There was low to moderate horizontal activity in bifenthrin and chlorfenapyr treatments, with no temperature effect in bifenthrin treatments and a positive temperature effect in chlorfenapyr treatments. Mortality in the fipronil treatments was highest and was positively correlated with temperature. Thiamethoxam treatments did not differ from controls at 10 degrees C, but mortality increased with temperature. To evaluate contact activity, either all of 20% of the ants in a cohort were exposed to insecticide-treated pine needles. In both tests, mortality was highest in fipronil and bifenthrin treatments, followed by thiamethoxam, with lowest mortality in chlorfenapyr treatments. Effectiveness as a barrier was evaluated by providing a choice between bridges treated with insecticide or water. Although bifenthrin did not provide an impenetrable barrier, it was the only treatment having fewer ants than its paired control. Mortality data suggest that lack of recruitment rather than repellency account for this result.

  16. Activity of bifenthrin, chlorfenapyr, fipronil, and thiamethoxam against red imported fire ants (Hymenoptera: Formicidae).

    Science.gov (United States)

    Wiltz, B A; Suiter, D R; Gardner, W A

    2010-06-01

    Bifenthrin, chlorfenapyr, fipronil, and thiamethoxam were evaluated for activity against the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae). Mobility impairment and lethal times were determined after topical treatments. Both immobilization and mortality occurred most quickly with bifenthrin, followed by thiamethoxam, chlorfenapyr, and fipronil. Mortality due to horizontal exposure was evaluated at 10, 20, or 30 degrees C, with three ratios of topically treated donor ant corpses to live recipients (5, 10, or 20% donors). Bifenthrin had the greatest horizontal activity of the chemicals tested. For chlorfenapyr, the only treatments having higher mortality than controls were the highest percentage donors at either 10 or 30 degrees C. Horizontal activity of fipronil was temperature dependent only with the highest proportion of donors and was lower than that ofbifenthrin but higher than that of chlorfenapyr or thiamethoxam. Mean mortality due to thiamethoxam was similar to that with chlorfenapyr. Significant mortality occurred in all of the 20 and 30 degrees C thiamethoxam treatments, but none of the 10 degrees C treatments. Effectiveness as a barrier was evaluated by providing a choice between bridges treated with insecticide or water. Although bifenthrin did not provide an impenetrable barrier, it was the only treatment having fewer ants than its paired control bridge. Mortality data suggest that a reduction in recruitment rather than repellency account for this result.

  17. The evolutionary dynamics of major regulators for sexual development among Hymenoptera species

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    Matthias eBiewer

    2015-04-01

    Full Text Available All hymenopteran species, such as bees, wasps and ants, are characterized by the common principle of haplodiploid sex determination in which haploid males arise from unfertilized eggs and females from fertilized eggs. The underlying molecular mechanism has been studied in detail in the western honey bee Apis mellifera, in which the gene complementary sex determiner (csd acts as primary signal of the sex determining pathway, initiating female development by csd-heterozygotes. Csd arose from gene duplication of the feminizer (fem gene, a transformer (tra ortholog, and mediates in conjunction with transformer2 (tra2 sex-specific splicing of fem. Comparative molecular analyses identified fem/tra and its downstream target doublesex (dsx as conserved unit within the sex determining pathway of holometabolous insects. In this study, we aim to examine evolutionary differences among these key regulators. Our main hypothesis is that sex determining key regulators in Hymenoptera species show signs of coevolution within single phylogenetic lineages. We take advantage of several newly sequenced genomes of bee species to test this hypothesis using bioinformatic approaches. We found evidences that duplications of fem are restricted to certain bee lineages and notable amino acid differences of tra2 between Apis and non-Apis species propose structural changes in Tra2 protein affecting co-regulatory function on target genes. These findings may help to gain deeper insights into the ancestral mode of hymenopteran sex determination and support the common view of the remarkable evolutionary flexibility in this regulatory pathway.

  18. Skeletal Morphology of Opius dissitus and Biosteres carbonarius (Hymenoptera: Braconidae), with a Discussion of Terminology

    Science.gov (United States)

    Karlsson, Dave; Ronquist, Fredrik

    2012-01-01

    The Braconidae, a family of parasitic wasps, constitute a major taxonomic challenge with an estimated diversity of 40,000 to 120,000 species worldwide, only 18,000 of which have been described to date. The skeletal morphology of braconids is still not adequately understood and the terminology is partly idiosyncratic, despite the fact that anatomical features form the basis for most taxonomic work on the group. To help address this problem, we describe the external skeletal morphology of Opius dissitus Muesebeck 1963 and Biosteres carbonarius Nees 1834, two diverse representatives of one of the least known and most diverse braconid subfamilies, the Opiinae. We review the terminology used to describe skeletal features in the Ichneumonoidea in general and the Opiinae in particular, and identify a list of recommend terms, which are linked to the online Hymenoptera Anatomy Ontology. The morphology of the studied species is illustrated with SEM-micrographs, photos and line drawings. Based on the examined species, we discuss intraspecific and interspecific morphological variation in the Opiinae and point out character complexes that merit further study. PMID:22558068

  19. Traumatic ventriculitis following consumption of introduced insect prey (Hymenoptera) in nestling hihi (Notiomystis cincta).

    Science.gov (United States)

    Rippon, Rosemary J; Alley, Maurice R; Castro, Isabel

    2013-01-01

    Nestling mortality in the endangered and endemic Hihi, also called Stitchbird (Notiomystis cincta), was studied over the 2008-09 breeding season at Zealandia-Karori Sanctuary, Wellington, New Zealand. Histopathology showed traumatic ventriculitis in seven of 25 (28%) dead nestlings. Single or multiple granulomas centered on chitinous insect remnants were found lodged within the gizzard mucosa, muscle layers, and ventricular or intestinal serosa. The insect remnants were confirmed as bee or wasp stings (Hymenoptera) using light and electron microscopy. Bacteria or yeasts were also found in some granulomas, and death was due to bacterial septicemia in four cases. Endemic New Zealand birds are likely to lack evolutionary adaptations required to safely consume introduced honey bees (Apis mellifera) and vespulid wasps (Vespula germanica [German wasp], and Vespula vulgaris [common wasp]). However, these insects are attracted to feeding stations used to support translocated Hihi populations. As contact between bees, wasps, and the endemic fauna of New Zealand seems inevitable, it may be necessary to minimize the numbers of these introduced insects in areas set aside for ecologic restoration.

  20. Costs of female odour in males of the parasitic wasp Lariophagus distinguendus (Hymenoptera: Pteromalidae)

    Science.gov (United States)

    Ruther, Joachim; Steiner, Sven

    2008-06-01

    The display of female traits by males is widespread in the animal kingdom. In several species, this phenomenon has been shown to function adaptively as a male mating strategy to deceive sexual rivals (female mimicry). Freshly emerged males of the parasitic wasp Lariophagus distinguendus (Hymenoptera: Pteromalidae) are perceived by other males as if they were females because of a very similar composition of cuticular hydrocarbons which function as a sex pheromone in this species inducing courtship behaviour in males. Within 32 h, however, males deactivate the pheromone and are no longer courted by other males. In this paper, behavioural experiments were performed to test hypotheses on potential costs and benefits associated with the female odour in young males. We did not find any benefits, but demonstrated that young males were significantly more often outrivaled in male-male contests when competing with two older males for a female. Also, young males were significantly more often mounted in homosexual courtship events during these contests. Thus, display of female traits by males is not necessarily beneficial, and in fact, can be disadvantageous. We suggest that these costs have favoured the evolution of the pheromone deactivation mechanism in L. distinguendus males. The function of cuticular hydrocarbons as a female courtship pheromone in L. distinguendus might have evolved secondarily from a primary function relevant for both genders, and the deactivation of the signal in males might have caused a shift of specificity of the chemical signal from the species level to the sex level.

  1. Lethal and sublethal effects of imidacloprid on Osmia lignaria and clothianidin on Megachile rotundata (Hymenoptera: Megachilidae).

    Science.gov (United States)

    Abbott, V A; Nadeau, J L; Higo, H A; Winston, M L

    2008-06-01

    We examined lethal and sublethal effects of imidacloprid on Osmia lignaria (Cresson) and clothianidin on Megachile rotundata (F.) (Hymenoptera: Megachilidae). We also made progress toward developing reliable methodology for testing pesticides on wild bees for use in pesticide registration by using field and laboratory experiments. Bee larvae were exposed to control, low (3 or 6 ppb), intermediate (30 ppb), or high (300 ppb) doses of either imidacloprid or clothianidin in pollen. Field experiments on both bee species involved injecting the pollen provisions with the corresponding pesticide. Only O. lignaria was used for the laboratory experiments, which entailed both injecting the bee's own pollen provisions and replacing the pollen provision with a preblended pollen mixture containing imidacloprid. Larval development, emergence, weight, and mortality were monitored and analyzed. There were no lethal effects found for either imidacloprid or clothianidin on O. lignaria and M. rotundata. Minor sublethal effects were detected on larval development for O. lignaria, with greater developmental time at the intermediate (30 ppb) and high doses (300 ppb) of imidacloprid. No similar sublethal effects were found with clothianidin on M. rotundata. We were successful in creating methodology for pesticide testing on O. lignaria and M. rotundata; however, these methods can be improved upon to create a more robust test. We also identified several parameters and developmental stages for observing sublethal effects. The detection of sublethal effects demonstrates the importance of testing new pesticides on wild pollinators before registration.

  2. Side-effects of pesticides used in irrigated rice areas on Telenomus podisi Ashmead (Hymenoptera: Platygastridae).

    Science.gov (United States)

    Pazini, Juliano de Bastos; Pasini, Rafael Antonio; Seidel, Enio Júnior; Rakes, Matheus; Martins, José Francisco da Silva; Grützmacher, Anderson Dionei

    2017-08-01

    Telenomus podisi Ashmead (Hymenoptera: Platygastridae) is an important agent for the biological control of stink bug eggs in irrigated rice areas and the best strategy for its preservation is the use of selective pesticides. The aim of this study was to know the side-effects of pesticides used in Brazilian irrigated rice areas on egg parasitoid T. podisi. We evaluated, under laboratory conditions, 13 insecticides, 11 fungicides, 11 herbicides, and a control (distilled water) in choice and no-choice tests. In the no-choice tests, the pesticides were sprayed at pre and post-parasitism stages (egg and larval stages of T. podisi). In the choice tests, sprays were conducted only at pre-parasitism stages. For all tests, we prepared cards with 25 eggs of the alternative host Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) non-parasitized (pre-parasitism) and parasitized (post-parasitism), which were subjected to pesticide sprays. The parasitism and emergence rates of T. podisi were determined classifying the pesticides in terms of the reduction of parasitism or emergence rates compared to the control. The neurotoxic insecticide cypermethrin, lambda-cyhalothrin, zeta-cypermethrin, etofenprox, thiamethoxam, thiamethoxam + lambda-cyhalothrin, acetamiprid + alpha-cypermethrin, and bifenthrin + alpha-cypermethrin + carbosulfan were more harmful to T. podisi and, therefore, are less suitable for the integrated management of insect pests in irrigated rice areas.

  3. Potential of ozone as a fumigant to control pests in honey bee (Hymenoptera: Apidae) hives.

    Science.gov (United States)

    James, R R

    2011-04-01

    Ozone is a powerful oxidant capable of killing insects and microorganisms, and eliminating odors, taste, and color. Thus, it could be useful as a fumigant to decontaminate honey comb between uses. The experiments here are intended to determine the exposure levels required to kill an insect pest and spore forming bee pathogens. Ozone was effective against greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae), even on naturally infested comb. Neonates and adults were the easiest life stages to kill, requiring only a few hours of exposure, whereas eggs required a 48-h exposure (at 460-920 mg O3/m3). Two honey bee, Apis mellifera L. (Hymenoptera: Apidae), pathogens, Ascosphaera apis (a fungus that causes chalkbrood) and Paenibacillus larvae (a bacterium that causes American foulbrood), also were killed with ozone. These pathogens required much higher concentrations (3200 and 8560 mg O3/m3, respectively) and longer exposure periods (3 d) than needed to control the insects. P. larvae was effectively sterilized only when these conditions were combined with high temperature (50 degrees C) and humidity (> or =75% RH). Thus, ozone shows potential as a fumigant for bee nesting materials, but further research is needed to evaluate its acceptability and efficacy in the field. The need for a reliable method to decontaminate honey bee nesting materials as part of an overall bee health management system is discussed.

  4. Function of the Dufour’s gland in solitary and social Hymenoptera

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    Aniruddha Mitra

    2013-10-01

    Full Text Available The poison gland and Dufour’s gland are the two glands associated with the sting apparatus in female Apocrita (Hymenoptera. While the poison gland usually functions as an integral part of the venom delivery system, the Dufour’s gland has been found to differ in its function in various hymenopteran groups. Like all exocrine glands, the function of the Dufour’s gland is to secrete chemicals, but the nature and function of the secretions varies in different taxa. Functions of the Dufour’s gland secretions range from serving as a component of material used in nest building, larval food, and pheromones involved in communicative functions that are important for both solitary and social species. This review summarizes the different functions reported for the Dufour’s gland in hymenopterans, illustrating how the Dufour’s gland secretions can be adapted to give rise to various functions in response to different challenges posed by the ways of life followed by different taxa. Aspects of development, structure, chemistry and the evolution of different functions are also touched upon briefly.

  5. Absence of complementary sex determination in the parasitoid wasp genus Asobara (Hymenoptera: Braconidae.

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    Wen-Juan Ma

    Full Text Available An attractive way to improve our understanding of sex determination evolution is to study the underlying mechanisms in closely related species and in a phylogenetic perspective. Hymenopterans are well suited owing to the diverse sex determination mechanisms, including different types of Complementary Sex Determination (CSD and maternal control sex determination. We investigated different types of CSD in four species within the braconid wasp genus Asobara that exhibit diverse life-history traits. Nine to thirteen generations of inbreeding were monitored for diploid male production, brood size, offspring sex ratio, and pupal mortality as indicators for CSD. In addition, simulation models were developed to compare these observations to predicted patterns for multilocus CSD with up to ten loci. The inbreeding regime did not result in diploid male production, decreased brood sizes, substantially increased offspring sex ratios nor in increased pupal mortality. The simulations further allowed us to reject CSD with up to ten loci, which is a strong refutation of the multilocus CSD model. We discuss how the absence of CSD can be reconciled with the variation in life-history traits among Asobara species, and the ramifications for the phylogenetic distribution of sex determination mechanisms in the Hymenoptera.

  6. Colony size evolution and the origin of eusociality in corbiculate bees (Hymenoptera: Apinae.

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    Enrique Rodriguez-Serrano

    Full Text Available Recently, it has been proposed that the one of the main determinants of complex societies in Hymenoptera is colony size, since the existence of large colonies reduces the direct reproductive success of an average individual, given a decreased chance of being part of the reproductive caste. In this study, we evaluate colony size evolution in corbiculate bees and their relationship with the sociality level shown by these bees. Specifically i the correlation between colony size and level of sociality considering the phylogenetic relationship to evaluate a general evolutionary tendency, and ii the hypothetical ancestral forms of several clades within a phylogeny of corbiculate bees, to address idiosyncratic process occurring at important nodes. We found that the level of social complexity in corbiculate bees is phylogenetically correlated with colony size. Additionally, another process is invoked to propose why colony size evolved concurrently with the level of social complexity. The study of this trait improves the understanding of the evolutionary transition from simple to complex societies, and highlights the importance of explicit probabilistic models to test the evolution of other important characters involved in the origin of eusociality.

  7. The foraging behavior of Diaeretiella rapae (Hymenoptera: Braconidae on Diuraphis noxia (Hemiptera: Aphididae

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    Tazerouni Zahra

    2011-01-01

    Full Text Available Host stage preference, functional response and mutual interference of Diaeretiella rapae (McIntosh (Hymenoptera: Braconidae: Aphidiinae on Diuraphis noxia (Mordvilko (Hemiptera: Aphididae were investigated under defined laboratory conditions (20±1°C; 60±5% relative humidity; 16 h light/8 h dark photoperiod. Nicholson’s model and linear regression were used to determine per capita search-efficiency and the interference coefficient, respectively. There was a significant difference between the rates of parasitism on different stages of D. noxia. The highest parasitism percentage was observed on the third instar nymphs of D. noxia in both choice and no-choice preference tests. Results of logistic regression revealed a type II functional response. The estimated values of search-efficiency (a and handling time (Th were 0.072 h-1 and 0.723 h, respectively. The maximum attack rate was calculated to be 33.22. The per capita search-efficiency decreased from 0.011 to 0.004 (h-1 as parasitoid densities increased from 1 to 8. Therefore, different host-parasitoid ratios can affect the efficacy of D. rapae.

  8. Feeding preferences of the willow sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae for commercial Salix clones

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    Verónica LOETTI

    2012-01-01

    Full Text Available Nematus oligospilus Förster (Hymenoptera: Tenthredinidae, o la avispa sierra de los sauces, es nativa del Hemisferio Norte y se ha convertido en un serio defoliador en plantaciones de sauces (Salix spp. del Hemisferio Sur, después de su introducción a principios de 1980. Los estudios sobre las preferencias de hospedador aportan información útil para el desarrollo de estrategias, donde la avispa sierra puede producir daño a los árboles y pérdidas económicas. Se evaluó la preferencia alimentaria de las larvas de N. oligospilus, mediante ensayos de laboratorio; se ofrecieron en forma simultánea hojas de cuatro clones de sauce, usados comúnmente en plantaciones comerciales en Argentina (Salix babylonica var sacramenta Hortus, Salix nigra Marsch., S. babylonica L. x Salix alba L. 131-27 and Salix matsudana Koidz. x S. alba L. 13-44. Las larvas de N. oligospilus se alimentaron de las hojas de los cuatro clones. Sin embargo, consumieron una proporción significativamente mayor de las hojas de S. babylonica var sacramenta. Estos resultados indican que todos los clones utilizados en los ensayos fueron palatables para el insecto y que S. babylonica var sacramenta es el hospedador preferido para la herbivoría de las larvas.

  9. The genus Macroteleia Westwood (Hymenoptera, Platygastridae s. l., Scelioninae from China

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    Chen Huayan

    2013-05-01

    Full Text Available The genus Macroteleia Westwood (Hymenoptera: Platygastridae s. l., Scelioninae from China is revised. Seventeen species are recognized based on 502 specimens, all of which are new records for China. Seven new species are described: M. carinigena sp. n. (China, M. flava sp. n. (China, M. gracilis sp. n. (China, M. salebrosa sp. n. (China, M. semicircula sp. n. (China, M. spinitibia sp. n. (China and M. striatipleuron sp. n. (China. Ten species are redescribed: M. boriviliensis Saraswat (China, India, Thailand, M. crawfordi Kiefer, stat. n. (China, Philippines, Thailand, Vietnam, M. dolichopa Sharma (China, India, Vietnam, M. emarginata Dodd (China, Malaysia, M. indica Saraswat & Sharma (China, India, Vietnam, M. lamba Saraswat & Sharma (China, India, Thailand, Vietnam, M. livingstoni Saraswat (China, India, M. peliades Kozlov & Lê (China, Vietnam, M. rufa Szelényi (China, Egypt, Georgia, Russia, Thailand, Ukraine and M. striativentris Crawford (China, Philippines, Thailand, Vietnam. The following five new synonyms are proposed: M. crates Kozlov & Lê syn. n. and M. demades Kozlov & Lê syn. n. of M. crawfordi Kieffer; M. cebes Kozlov & Lê syn. n. and M. dones Kozlov & Lê syn. n. of M. indica Saraswat & Sharma; M. dores Kozlov & Lê syn. n. of M. lamba Saraswat & Sharma. A key to the Chinese species of the genus is provided.

  10. Guidelines for Clinical Practice: Hymenoptera sting allergy in children: 2017 update

    Science.gov (United States)

    2017-10-01

    Las picaduras por himenópteros son frecuentes en la infancia. La mayoría producen reacciones locales, consecuencia de un mecanismo inflamatorio, no alérgico, no tienen progresión y se resuelven con simples medidas terapéuticas. Las reacciones más extensas, generalmente, están asociadas a mecanismos alérgicos, mediados por inmunoglobulina E. Su máxima expresión, la anafilaxia, presenta síntomas cutáneos, respiratorios, cardiovasculares, digestivos y/o neurológicos, con riesgo de muerte. La prevalencia de anafilaxia en pacientes con mastocitosis sistémica es más alta. La familia Hymenoptera, que incluye hormigas coloradas, abejas y avispas, es la causante de las picaduras de mayor riesgo, potencialmente fatales. Los pilares del diagnóstico son la historia clínica, la identificación del insecto, y las pruebas diagnósticas cutáneas y/o in vitro interpretadas por el especialista en Alergia e Inmunología. La inmunoterapia con veneno es el tratamiento de elección para prevenir reacciones anafilácticas por picaduras de himenópteros.

  11. Mosaicism may explain the evolution of social characters in haplodiploid Hymenoptera with female workers.

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    Morpurgo, Giorgio; Babudri, Nora; Fioretti, Bernard; Catacuzzeno, Luigi

    2010-12-01

    The role of haplodiploidy in the evolution of eusocial insects and why in Hymenoptera males do not perform any work is presently unknown. We show here that within-colony conflict caused by the coexistence of individuals of the same caste expressing the same character in different ways can be fundamental in the evolution of social characters in species that have already reached the eusocial condition. Mosaic colonies, composed by individuals expressing either the wild-type or a mutant phenotype, inevitably occurs during the evolution of advantageous social traits in insects. We simulated the evolution of an advantageous social trait increasing colony fitness in haplodiploid and diplodiploid species considering all possible conditions, i.e. dominance/recessivity of the allele determining the new social character, sex of the castes, and influence of mosaicism on the colony fitness. When mosaicism lowered colony fitness below that of the colony homogeneous for the wild type allele, the fixation of an advantageous social character was possible only in haplodiploids with female castes. When mosaicism caused smaller reductions in colony fitness, reaching frequencies of 90% was much faster in haplodiploids with female castes and dominant mutations. Our results suggest that the evolution of social characters is easier in haplodiploid than in diplodiploid species, provided that workers are females.

  12. Studi berbagai jenis sarang permanen untuk mengembangbiakkan semut hitam, Dolichoderus thoracicus (Smith (Hymenoptera: Formicidae

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    Ahmad Saleh

    2015-09-01

    Full Text Available Black ants, Dolichoderus thoracicus (Smith (Hymenoptera: Formicidae is one of the biological agents that can be used to control the cocoa pod borer Conopomorpha cramerella and Helopeltis sp. To effectively prevent C. cramerella infestation, high population of black ants are required. Population growth of black ants in three types of permanent nests and non permanent nest were observed during 3 months. Every month, five nests of each type were taken at random and then all stadia were calculated. The permanent nests will support ant population for some years and the black ant population per nest is higher when compared to nest made of dried cocoa leaves only. Permanent nests are made of 75 pieces of dried cocoa leaves which are placed in plastic bags with ventilation holes. In the longer term permanent black ant nests are more cost effective then ant nests which have been made from only dried leaves because frequent replacement is not required. Permanent ant nests will also ensure a stable black ant population is maintained.

  13. Toxicity of Pesticide Tank Mixtures from Rice Crops Against Telenomus podisi Ashmead (Hymenoptera: Platygastridae).

    Science.gov (United States)

    de B Pazini, J; Pasini, R A; Rakes, M; de Armas, F S; Seidel, E J; da S Martins, J F; Grützmacher, A D

    2017-08-01

    The use of insecticides, herbicides, and fungicides commonly occurs in mixtures in tanks in order to control phytosanitary problems in crops. However, there is no information regarding the effects of these mixtures on non-target organisms associated to the rice agroecosystem. The aim of this study was to know the toxicity of pesticide tank mixtures from rice crops against Telenomus podisi Ashmead (Hymenoptera: Platygastridae). Based on the methods adapted from the International Organisation for Biological and Integrated Control of Noxious Animals and Plants (IOBC), adults of T. podisi were exposed to residues of insecticides, herbicides, and fungicides, individually or in mixture commonly used by growers, in laboratory and on rice plants in a greenhouse. The mixture between fungicides tebuconazole, triclyclazole, and azoxystrobin and the mixture between herbicides cyhalofop-butyl, imazethapyr, imazapyr/imazapic, and penoxsulam are harmless to T. podisi and can be used in irrigated rice crops without harming the natural biological control. The insecticides cypermethin, thiamethoxam, and bifenthrin/carbosulfan increase the toxicity of the mixtures in tank with herbicides and fungicides, being more toxic to T. podisi and less preferred for use in phytosanitary treatments in the rice crop protection.

  14. Development of virtual bait stations to control Argentine ants (Hymenoptera: Formicidae) in environmentally sensitive habitats.

    Science.gov (United States)

    Choe, Dong-Hwan; Vetter, Richard S; Rust, Michael K

    2010-10-01

    A novel bait station referred to as a virtual bait station was developed and tested against field populations of the invasive Argentine ant, Linepithema humile (Mayr) (Hymenoptera: Formicidae), at White Beach, Camp Pendleton, in Oceanside, CA. White Beach is a nesting habitat for an endangered seabird, the California least tern (Sterna antillarum browni Mearns). The beach is heavily infested with Argentine ants, one of the threats for the California least tern chicks. Conventional pest control strategies are prohibited because of the existence of the protected bird species and the site's proximity to the ocean. The bait station consisted of a polyvinyl chloride pipe that was treated on the inside with fipronil insecticide at low concentrations to obtain delayed toxicity against ants. The pipe was provisioned with an inverted bottle of 25% sucrose solution, then capped, and buried in the sand. Foraging ants crossed the treated surface to consume the sucrose solution. The delayed toxicity of fipronil deposits allowed the ants to continue foraging on the sucrose solution and to interact with their nestmates, killing them within 3-5 d after exposure. Further modification of the bait station design minimized the accumulation of dead ants in the sucrose solution, significantly improving the longevity and efficacy of the bait station. The virtual bait station exploits the foraging behavior of the ants and provides a low impact approach to control ants in environmentally sensitive habitats. It excluded all insects except ants, required only milligram quantities of toxicant, and eliminated the problem of formulating toxicants into aqueous sugar baits.

  15. A Landscape Analysis to Understand Orientation of Honey Bee (Hymenoptera: Apidae) Drones in Puerto Rico.

    Science.gov (United States)

    Galindo-Cardona, A; Monmany, A C; Diaz, G; Giray, T

    2015-08-01

    Honey bees [Apis mellifera L. (Apidae, Hymenoptera)] show spatial learning behavior or orientation, in which animals make use of structured home ranges for their daily activities. Worker (female) orientation has been studied more extensively than drone (male) orientation. Given the extensive and large flight range of drones as part of their reproductive biology, the study of drone orientation may provide new insight on landscape features important for orientation. We report the return rate and orientation of drones released at three distances (1, 2, and 4 km) and at the four cardinal points from an apiary located in Gurabo, Puerto Rico. We used high-resolution aerial photographs to describe landscape characteristics at the releasing sites and at the apiary. Analyses of variance were used to test significance among returning times from different distances and directions. A principal components analysis was used to describe the landscape at the releasing sites and generalized linear models were used to identify landscape characteristics that influenced the returning times of drones. Our results showed for the first time that drones are able to return from as far as 4 km from the colony. Distance to drone congregation area, orientation, and tree lines were the most important landscape characteristics influencing drone return rate. We discuss the role of landscape in drone orientation. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  16. Survival of honey bee (Hymenoptera: Apidae) spermatozoa incubated at room temperature from drones exposed to miticides.

    Science.gov (United States)

    Burley, Lisa M; Fell, Richard D; Saacke, Richard G

    2008-08-01

    We conducted research to examine the potential impacts ofcoumaphos, fluvalinate, and Apilife VAR (Thymol) on drone honey bee, Apis mellifera L. (Hymenoptera: Apidae), sperm viability over time. Drones were reared in colonies that had been treated with each miticide by using the dose recommended on the label. Drones from each miticide treatment were collected, and semen samples were pooled. The pooled samples from each treatment were subdivided and analyzed for periods of up to 6 wk. Random samples were taken from each treatment (n = 6 pools) over the 6-wk period. Sperm viability was measured using dual-fluorescent staining techniques. The exposure of drones to coumaphos during development and sexual maturation significantly reduced sperm viability for all 6 wk. Sperm viability significantly decreased from the initial sample to week 1 in control colonies, and a significant decrease in sperm viability was observed from week 5 to week 6 in all treatments and control. The potential impacts of these results on queen performance and failure are discussed.

  17. Diversity of Braconidae (Insecta, Hymenoptera of the Parque Natural Municipal de Porto Velho, Rondonia, Brazil

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    Sian de Souza Gadelha

    2012-12-01

    Full Text Available Braconidae is a highly diversified family of Hymenoptera and usually known by their role in biological control both in agricultural and natural ecosystems. Despite of that, little is known about its diversity in the Amazon region. The present work inventoried the braconid fauna of an Open Ombrophylous Forest with Palm Trees of the Parque Natural Municipal de Porto Velho, RO. Insects were collect from June/2008 to May/2009 using six Malaise traps in different parts of the reserve. A total of 377 wasps were captured, 17 subfamilies and 56 genera identified. Braconinae, Microgastrinae, Doryctinae and Rogadinae subfamilies were very abundant, and also the genera Aleiodes, Bracon, Capitonius, Compsobracon, Heterospilus, Hymenochaonia, Opius, Pedinotus, Rogas and Stantonia. The calculated Shannon diversity index was 2.15 and 3.3 for subfamily and genera, respectively, which were, generally, higher than the values found for other regions in Brazil. Generally, parasitoids were more abundant during the rainy season. The present work contributes with new genera records and faunistic data of Braconidae in Rondonia State, western Amazon.

  18. Go High or Go Low? Adaptive Evolution of High and Low Relatedness Societies in Social Hymenoptera

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    Peter Nonacs

    2017-08-01

    Full Text Available Cooperative groups can increase fitness either by helping kin or interacting with unlike individuals to produce social heterosis. They cannot, however, simultaneously maximize both benefits. This tradeoff between nepotism and diversity is modeled using Hamilton's rule (rb–c > 0, by allowing benefit and cost to be dynamic functions of relatedness (i.e., social heterosis predicts b and c depend on r. Simulations show that evolutionary outcomes tend to maximize either nepotism (with high genetic relatedness, or social heterosis (with low relatedness rather than produce an intermediate outcome. Although genetic diversity can arise through multiple mating, a second possible mechanism—the exchanging of individuals across groups—is similarly effective. Such worker “drifting” is common in many species of social Hymenoptera and may be a form of indirect reciprocity. Drifting individuals increase an unrelated group's productivity by enhancing its genetic diversity, with this effect being reciprocated by other unrelated drifters entering their natal group. The benefits from social heterosis and indirect reciprocity are robust against cheating and show that it is possible to evolve stable cooperation between individuals that are genetically distant or unrelated. As drifting becomes more prevalent colony boundaries may become weakly discriminated, which may predispose toward the evolution of unicoloniality in some species.

  19. Fortuitous establishment of Ageniaspis citricola (Hymenoptera: Encyrtidae) in Jamaica on the citrus leafminer (Lepidoptera: Gracillariidae)

    International Nuclear Information System (INIS)

    Hoy, M.A.; Jeyaprakash, A.; Clarke-Harris, D.

    2007-01-01

    These data indicate that the population of A. citricola in Jamaica probably originated from the Australian (Thailand), rather than from the Taiwan, population. This is consistent with what is currently known about the origin of the established Ageniaspis population in Florida (Alvarez 2000). It is not known when, or how, A. citricola arrived in Jamaica, although the CLM was detected there in 1994. The fortuitous establishment of A. citricolaon the CLM in Jamaica is not the only such establishment of a natural enemy discovered during this 2004 survey of citrus. The parasitoid Lipolexis oregmae Gahan (Hymenoptera: Aphidiidae) was found attacking the brown citrus aphid, Toxoptera citricida Kirkaldy (Hemiptera: Aphididae) (Hoy et al., unpublished data), and the eulophid parasitoid Tamarixia radiata Waterston was found attacking the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae). The fact that 3 natural enemies of 3 invasive citrus pests were found in Jamaica, none of which were purposefully imported and released, suggests that pest-infested citrus trees were imported into Jamaica without going through appropriate quarantine procedures. Because each pest arrived at different times, the parasitoids probably arrived at different times, as well. This indicates that an analysis is needed to identify the critical control points within those services in Jamaica that support border protection, and that procedures may require strengthening. (author)

  20. Compatibility of endoparasitoid Hyposoter didymator (Hymenoptera: Ichneumonidae) protected stages with five selected insecticides.

    Science.gov (United States)

    Medina, P; Morales, J J; Budia, F; Adan, A; Del Estal, P; Viñuela, E

    2007-12-01

    Hyposoter didymator (Thunberg) (Hymenoptera: Ichneumonidae) is a koinobiont endoparasitoid that emerges from the parasitization of economically important noctuid pests. H. didymator also is considered one of the most important native biocontrol agents of noctuids in Spain. Side effects of five insecticides with very different modes of action (fipronil, imidacloprid, natural pyrethrins + piperonyl butoxide, pymetrozine, and triflumuron) at the maximum field recommended rate in Spain were evaluated on H. didymator parasitizing Spodoptera littoralis (Boisduval) larvae and pupae of the endoparasitoid. Parasitized larvae were topically treated or ingested treated artificial diet. Parasitoid cocoons were topically treated. Host mortality when parasitized larvae were treated, as well as further development of the parasitoid surviving (e.g., percentage of cocoons spun, adult emergence, hosts attacked, and numbered progeny) were determined. Toxicity after treatment of parasitized larvae differed depending on the mode of exposure and insecticide. Fipronil was always highly toxic; imidacloprid killed all host insects by ingestion, but it was less toxic to both host and parasitoids, when administered topically; natural pyrethrins + piperonyl butoxide and triflumuron showed differing degrees of toxicity, and pymetrozine was harmless. Parasitoid cocoons provided effective protection against all the insecticides, except fipronil.

  1. Sublethal Effects of the Neonicotinoid Insecticide Thiamethoxam on the Transcriptome of the Honey Bees (Hymenoptera: Apidae).

    Science.gov (United States)

    Shi, Teng-Fei; Wang, Yu-Fei; Liu, Fang; Qi, Lei; Yu, Lin-Sheng

    2017-12-05

    Neonicotinoid insecticides are now the most widely used insecticides in the world. Previous studies have indicated that sublethal doses of neonicotinoids impair learning, memory capacity, foraging, and immunocompetence in honey bees (Apis mellifera, Linnaeus) (Hymenoptera: Apidae). Despite these, few studies have been carried out on the molecular effects of neonicotinoids. In this study, we focus on the second-generation neonicotinoid thiamethoxam, which is currently widely used in agriculture to protect crops. Using high-throughput RNA-Seq, we investigated the transcriptome profile of honey bees after subchronic exposure to 10 ppb thiamethoxam over 10 d. In total, 609 differentially expressed genes (DEGs) were identified, of which 225 were upregulated and 384 were downregulated. Several genes, including vitellogenin, CSP3, defensin-1, Mrjp1, and Cyp6as5 were selected and further validated using real-time quantitative polymerase chain reaction assays. The functions of some DEGs were identified, and Gene Ontology-enrichment analysis showed that the enriched DEGs were mainly linked to metabolism, biosynthesis, and translation. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that thiamethoxam affected biological processes including ribosomes, the oxidative phosphorylation pathway, tyrosine metabolism pathway, pentose and glucuronate interconversions, and drug metabolism. Overall, our results provide a basis for understanding the molecular mechanisms of the complex interactions between neonicotinoid insecticides and honey bees. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  2. Leucophora Satellite Flies (Diptera: Anthomyiidae) as Nest Parasites of Sweat Bees (Hymenoptera: Halictidae) in the Neotropics.

    Science.gov (United States)

    Polidori, C; Michelsen, V; Nieves-Aldrey, J L

    2015-08-01

    The biology of the 10 species of Leucophora (Diptera: Anthomyiidae) recorded in the Neotropics remains unknown. The large majority of the studied species so far are kleptoparasites of bees and wasps. Here, we report the first observations of Leucophora andicola (Bigot) and Leucophora peullae (Malloch) visiting the nests of ground-nesting sweat bees Corynura (Hymenoptera: Halictidae) in Chilean Patagonia. Females of both species perch on small stones or sticks within a dense nest aggregation of the bees and then track pollen-loaded bees in flight with great precision, eventually following them into their nests. The overall behavior closely resembles that observed for many other species of the genus. Excavations of some bee nests returned only two dipteran puparia, possibly of Leucophora, suggesting a low parasitism rate. One male of L. peullae was also collected at the bee aggregation. This is the first report of host association for any Leucophora from the Neotropics and the first report of any anthomyiid fly associated with augochlorine bees.

  3. Health status of alfalfa leafcutting bee larvae (Hymenoptera: Megachilidae) in United States alfalfa seed fields.

    Science.gov (United States)

    James, R R; Pitts-Singer, T L

    2013-12-01

    We conducted a broad geographic survey in the northwestern United States to quantify production losses in the alfalfa leafcutting bee (Megachile rotundata (F.), Hymenoptera: Megachilidae), a solitary pollinator used extensively in alfalfa seed production. Viable larvae were found in only 47.1% of the nest cells collected at the end of the season. Most of the rest of the cells contained pollen balls (typified by a provision but no larva; 16.7%), unknown causes of mortality (15.5%), or larvae killed by chalkbrood (8.0%). Prevalence of pollen balls was correlated positively with bee release rates and negatively with alfalfa stand age. The unknown mortality was correlated with the U.S. Department of Agriculture-Plant Hardiness Zone, and thus, some of the mortality may be caused by high temperature extremes, although the nesting season degree-days were not correlated with this mortality. Chalkbrood prevalence was correlated with possible nesting-resource or crowding-related factors, such as the number of bees released per hectare and the number of shelters used, but not with nesting board disinfection practices. Vapona is used to control parasitoids when the parent bees are incubated before release, and use of this fumigant was associated with an increase in both chalkbrood and diapausing offspring, although any reason for these correlations are unknown. This survey quantifies the variation in the quality of alfalfa leafcutting bee cocoons produced across much of the U.S. alfalfa seed production area.

  4. BAP, 2,4-D e ácido acetilsalicílico na indução e diferenciação de calos em anteras de Coffea arabica L BAP, 2,4-D and acetyl-salicylic acid on the callus induction and differentiation in Coffea arabica L. anthers

    Directory of Open Access Journals (Sweden)

    Adelaide Siqueira Silva

    2009-10-01

    Full Text Available O melhoramento genético do cafeeiro por meio de métodos convencionais é um processo demorado para se obter uma nova cultivar. A redução desse tempo é possível através da produção de linhagens homozigóticas, oriundas de dihaplóides obtidas através da cultura de anteras. Objetivou-se aplicar a técnica da cultura de anteras em diferentes cvs. de Coffea arabica L. para induzir a formação de calos e regenerar plântulas di-haplóides, com uso de reguladores vegetais. Os experimentos foram conduzidos no laboratório de Biotecnologia Vegetal da Universidade Federal de Uberlândia (UFU. Anteras das cultivares Mundo Novo LCP-379-19 e Catuaí Vermelho H2077-2-5-44 foram inoculadas em meio MS suplementado com 2,0 mg L-1 de 2,4-D e AAS, nas concentrações de 0; 8; 16; 32 e 64 mg L-1. Calos de 'Catuaí Vermelho 44' foram subcultivados em meio MS acrescido de diferentes concentrações de BAP (0; 2; 4 e 8 mg L-1 e 2,4-D (0; 1; 2 e 4 mg L-1. Tanto para as cvs. Mundo Novo quanto para Catuaí Vermelho 44 o aumento das concentrações de AAS diminuiu a formação de próembrióides nos calos e somente o 2,4-D foi capaz de promover a formação de calos friáveis, porém o equilíbrio da auxina e da citocinina utilizadas no trabalho, favoreceram a produção de calos friáveis.Coffee plant breeding through conventional methods demands a long time to obtain new cultivars. The reduction of this period is possible through the production of homozygous lines, from dihaploids obtained via anther culture. The aim of this study was to apply the anther culture technique on different C. arabica L. cultivars to induce calli formation and to regenerate dihaploid seedlings with the use of plant growth regulators. The experiments were accomplished in the Plant Biotechnology laboratory at Uberlândia Federal University (UFU. Anthers of the cultivars Mundo Novo LCP-379-19 and Catuaí Vermelho H2077-2-5-44 were inoculated on MS medium supplemented with 2.0 mg L-1

  5. The genus Odontocynips Kieffer, 1910 (Hymenoptera, Cynipidae, Cynipini in Panama, with redescription of Cynips championi Cameron, 1883

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    Pujade-Villar, J.

    2011-06-01

    Full Text Available The genus Odontocynips Kieffer, 1910 (Hymenoptera, Cynipidae, Cynipini is recorded for the first time in Panama, including two species, O. championi (Cameron and O. hansoni Pujade-Villar, that induce galls on Quercus bumelioides Liebm. and Q. lancifolia Schledl & Cham. (Fagaceae, sect. Quercus, White Oaks, respectively. Odontocynips championi (Cameron, 1833, originally described as Cynips championi Cameron based solely on galls, is redescribed including the first description of the adults, a neotype is designated and a new combination is established. The known distribution and host range of O. hansoni, recorded earlier from Costa Rica, are also expanded upon.Se cita por primera vez para Panamá el género Odontocynips Kieffer, 1910 (Hymenoptera, Cynipidae, Cynipini, incluyendo dos especies: Odontocynips championi (Cameron y O. hansoni Pujade-Villar, que inducen agallas en Quercus bumelioides Liebm. y Q. lancifolia Schledl & Cham. (Fagaceae, sect. Quercus, robles blancos, respectivamente. Se redescribe Odontocynips championi (Cameron, 1833, descrita solo a partir de sus agallas como Cynips championi Cameron, se describen por primera vez los adultos, se designa un neotipo y se establece una nueva combinación taxonómica al transferirla al género Odontocynips. Por otra parte, se amplía la distribución geográfica y rango de hospedador de O. hansoni, previamente citada sólo de Costa Rica.

  6. First successful case of in vitro fertilization-embryo transfer with venom immunotherapy for hymenoptera sting allergy

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    Tucker Michael J

    2004-10-01

    Full Text Available Abstract Background To describe immune and endocrine responses in severe hymenoptera hypersensitivity requiring venom immunotherapy (VIT during in vitro fertilization (IVF. Case presentation A 39-year old patient was referred for history of multiple miscarriage and a history of insect sting allergy. Four years earlier, she began subcutaneous injection of 100 mcg mixed vespid hymenoptera venom/venom protein every 5–6 weeks. The patient had one livebirth and three first trimester miscarriages. Allergy treatment was maintained for all pregnancies ending in miscarriage, although allergy therapy was discontinued for the pregnancy that resulted in delivery. At our institution ovulation induction incorporated venom immunotherapy (VIT during IVF, with a reduced VIT dose when pregnancy was first identified. Serum IgE was monitored with estradiol during ovulation induction and early pregnancy. Response to controlled ovarian hyperstimulation was favorable while VIT was continued, with retrieval of 12 oocytes. Serum RAST (yellow jacket IgE levels fluctuated in a nonlinear fashion (range 36–54% during gonadotropin therapy and declined after hCG administration. A healthy female infant was delivered at 35 weeks gestation. The patient experienced no untoward effects from any medications during therapy. Conclusion Our case confirms the safety of VIT in pregnancy, and demonstrates RAST IgE can remain

  7. Morphological and Chemical Characterization of the Invasive Ants in Hives of Apis mellifera scutellata Lepeletier (Hymenoptera: Apidae).

    Science.gov (United States)

    Simoes, M R; Giannotti, E; Tofolo, V C; Pizano, M A; Firmino, E L B; Antonialli-Junior, W F; Andrade, L H C; Lima, S M

    2016-02-01

    Apiculture in Brazil is quite profitable and has great potential for expansion because of the favorable climate and abundancy of plant diversity. However, the occurrence of pests, diseases, and parasites hinders the growth and profitability of beekeeping. In the interior of the state of São Paulo, apiaries are attacked by ants, especially the species Camponotus atriceps (Smith) (Hymenoptera: Formicidae), which use the substances produced by Apis mellifera scutellata (Lepeletier) (Hymenoptera: Apidae), like honey, wax, pollen, and offspring as a source of nourishment for the adult and immature ants, and kill or expel the adult bees during the invasion. This study aimed to understand the invasion of C. atriceps in hives of A. m. scutellata. The individuals were classified into castes and subcastes according to morphometric analyses, and their cuticular chemical compounds were identified using Photoacoustic Fourier transform infrared spectroscopy (FTIR-PAS). The morphometric analyses were able to classify the individuals into reproductive castes (queen and gynes), workers (minor and small ants), and the soldier subcaste (medium and major ants). Identification of cuticular hydrocarbons of these individuals revealed that the eight beehives were invaded by only three colonies of C. atriceps; one of the colonies invaded only one beehive, and the other two colonies underwent a process called sociotomy and were responsible for the invasion of the other seven beehives. The lack of preventive measures and the nocturnal behavior of the ants favored the invasion and attack on the bees.

  8. Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae).

    Science.gov (United States)

    Chen, Long; Chen, Peng-Yan; Xue, Xiao-Feng; Hua, Hai-Qing; Li, Yuan-Xi; Zhang, Fan; Wei, Shu-Jun

    2018-05-04

    Animal mitochondrial genomes usually exhibit conserved gene arrangement across major lineages, while those in the Hymenoptera are known to possess frequent rearrangements, as are those of several other orders of insects. Here, we sequenced two complete mitochondrial genomes of Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae). In total, 37 mitochondrial genes were identified in both species. The same gene arrangement pattern was found in the two species, with extensive gene rearrangement compared with the ancestral insect mitochondrial genome. Most tRNA genes and all protein-coding genes were encoded on the minority strand. In total, 15 tRNA genes and seven protein-coding genes were rearranged. The rearrangements of cox1 and nad2 as well as most tRNA genes were novel. Phylogenetic analysis based on nucleotide sequences of protein-coding genes and on gene arrangement patterns produced identical topologies that support the relationship of (Agaonidae + Pteromalidae) + Trichogrammatidae in Chalcidoidea. CREx analysis revealed eight rearrangement operations occurred from presumed ancestral gene order of Chalcidoidea to form the derived gene order of Trichogramma. Our study shows that gene rearrangement information in Chalcidoidea can potentially contribute to the phylogeny of Chalcidoidea when more mitochondrial genome sequences are available.

  9. Progeny Density and Nest Availability Affect Parasitism Risk and Reproduction in a Solitary Bee (Osmia lignaria) (Hymenoptera: Megachilidae).

    Science.gov (United States)

    Farzan, Shahla

    2018-02-08

    Gregarious nesting behavior occurs in a broad diversity of solitary bees and wasps. Despite the prevalence of aggregative nesting, the underlying drivers and fitness consequences of this behavior remain unclear. I investigated the effect of two key characteristics of nesting aggregations (cavity availability and progeny density) on reproduction and brood parasitism rates in the blue orchard bee (Osmia lignaria Say) (Hymenoptera: Megachilidae), a solitary species that nests gregariously and appears to be attracted to nesting conspecifics. To do so, I experimentally manipulated nest cavity availability in a region of northern Utah with naturally occurring populations of O. lignaria. Nest cavity availability had a negative effect on cuckoo bee (Stelis montana Cresson) (Hymenoptera: Megachilidae) parasitism rates, with lower parasitism rates occurring in nest blocks with more available cavities. For both S. montana and the cleptoparasitic blister beetle Tricrania stansburyi Haldeman (Coleoptera: Meloidae), brood parasitism rate was negatively correlated with log-transformed O. lignaria progeny density. Finally, cavity availability had a positive effect on male O. lignaria body weight, with the heaviest male progeny produced in nest blocks with the most cavities. These results suggest that cavity availability and progeny density can have substantial effects on brood parasitism risk and reproduction in this solitary bee species. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  10. The wasp larva's last supper: 100 million years of evolutionary stasis in the larval development of rhopalosomatid wasps (Hymenoptera: Rhopalosomatidae

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    V. Lohrmann

    2017-08-01

    Full Text Available Rhopalosomatidae are an unusual family of wasps (Hymenoptera: Aculeata comprising less than 100 species found in the tropics and subtropics of all continents except Europe and Antarctica. Whereas some species resemble nocturnal Ichneumonidae, others might be mistaken for spider wasps or different groups of brachypterous Hymenoptera. Despite their varied morphology, all members of the family supposedly develop as larval ectoparasitoids of crickets (Orthoptera: Grylloidea. Here, we report on the first record of a fossil rhopalosomatid larva which was discovered in mid-Cretaceous amber from northern Myanmar (Burma. The larva is attached to the lateral side of a cricket between the metafemur and the abdomen, impacting the natural position of the hind leg, exactly as documented for modern species. Additionally, the larval gestalt is strikingly similar to those of extant forms. These observations imply that this behavioral specialization, e.g., host association and positioning on host, likely evolved in the stem of the family at least 100 million years ago.

  11. Evaluación de la incidencia de la aplicación foliar de ácido giberélico en la floración de árboles de coffea arabica l. y su impacto frente al cambio climático

    OpenAIRE

    Zapata Restrepo, Leidy Natalia

    2013-01-01

    Tesis (Maestría en Desarrollo Sostenible y Medio Ambiente). Universidad de Manizales. Facultad de Ciencias Contables, Económicas y Administrativas, 2013 La giberelina es conocida como una fitohormona que participa activamente en el control de la floración de varias especies. En el presente estudio se evaluó la aplicación exógena de ácido giberélico (0.1% y 0.5%) a 2959 plantas de Coffea arabica L., variedad Castillo cultivar Rosario con el Objetivo de establecer la relación existente entr...

  12. Famílias de Hymenoptera (Insecta como ferramenta avaliadora da conservação de restingas no extremo sul do Brasil

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    Cristina Maria Loyola Zardo

    2009-12-01

    Full Text Available Com o objetivo de estimar e comparar a diversidade e flutuação populacional das famílias de Hymenoptera em área de restinga foi realizado um levantamento faunístico em duas áreas de restinga com diferentes níveis de conservação. Foram coletados 5.518 himenópteros distribuídos em 30 famílias. Os picos populacionais na flutuação das famílias ocorreram no verão confirmando a alta correlação da temperatura com a distribuição das famílias. Constatou-se na restinga em sucessão maior riqueza, porém, com alta dominância, abrigando representantes dos três grupos ecológicos (antófilos, generalistas e parasitóides em alta abundância. A restinga preservada, com 17 famílias, verificou-se mais diversa e homogênea, onde verificou-se maior abundância dos parasitóides, devido à maior estabilidade do sistema. A riqueza de famílias de Hymenoptera em áreas de restinga pode ser utilizada como parâmetro indicativo de qualidade ambiental, para este tipo de bioma.Hymenoptera Families (Insecta as Evaluation Tool of the Conservation of Sandbanks in Southern BrazilAbstract. With aim to estimate and compare the diversity and population of the Hymenoptera families in a sandbank area was carried out a wildlife survey in two areas of sandbank with different levels of conservation. We collected 5 518 Hymenoptera distributed in 30 families. The peaks in the families fluctuation occurred in the summer confirmed the high correlation of temperature with the distribution of families. The sandbank in succession had the highest richness, however with high dominance, hosting representatives of the three ecological groups (anthophilous, generalists and parasitoids in high abundance. The sandbank preserved, with 17 families, was more diverse and homogeneous, where the parasitoids showed greater abundance due to greater system stability. The richness of Hymenoptera families in sandbanks can be used as a parameter indicative of environmental quality.

  13. Nota sobre o comportamento de agregação dos machos de Oxaea austera Gerstaecker (Hymenoptera, Apoidea, Oxaeinae na caatinga do Estado da Bahia, Brasil Notes on a male sleeping aggregation behavior of Oxaea austera Gerstaecker (Hymenoptera, Apoidea, Oxaeinae in the caatinga of Bahia State, Brazil

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    Favízia Freitas de Oliveira

    2002-03-01

    Full Text Available This note reports for the first time a "male sleeping aggregation" of the solitary bee Oxaea austera Gerstaecker, 1867 (Hymenoptera, Apoidea, Oxaeinae found near the town of Iaçú, Bahia, in Northeastern Brazil. This is also the first record of a species of Oxaea for the caatinga ecosystem.

  14. Optimizing Drone Fertility With Spring Nutritional Supplements to Honey Bee (Hymenoptera: Apidae) Colonies.

    Science.gov (United States)

    Rousseau, Andrée; Giovenazzo, Pierre

    2016-03-27

    Supplemental feeding of honey bee (Apis melliferaL., Hymenoptera: Apidae) colonies in spring is essential for colony buildup in northern apicultural regions. The impact of pollen and syrup feeding on drone production and sperm quality is not well-documented, but may improve fecundation of early-bred queens. We measured the impact of feeding sucrose syrup, and protein supplements to colonies in early spring in eastern Canada. Drones were reared under different nutritional regimes, and mature individuals were then assessed in regard to size, weight, and semen quality (semen volume, sperm count, and viability). Results showed significant increases in drone weight and abdomen size when colonies were fed sucrose and a protein supplement. Colonies receiving no additional nourishment had significantly less semen volume per drone and lower sperm viability. Our study demonstrates that feeding honey bee colonies in spring with sucrose syrup and a protein supplement is important to enhance drone reproductive quality. RÉSUMÉ: L'administration de suppléments alimentaires aux colonies de l'abeille domestique (Apis melliferaL., Hymenoptera: Apidae) au printemps est essentielle pour le bon développement des colonies dans les régions apicoles nordiques. L'impact de la supplémentation des colonies en pollen et en sirop sur la production des faux-bourdons et la qualité du sperme demeure peu documenté mais pourrait résulter en une meilleure fécondation des reines produites tôt en saison. Nous avons mesuré l'impact de la supplémentation en sirop et/ou en supplément de pollen sur les colonies d'abeilles tôt au printemps dans l'est du Canada. Les faux-bourdons ont été élevé sous différents régimes alimentaires et les individus matures ont ensuite été évalués pour leur taille, leur poids ainsi que la qualité de leur sperme (volume de sperme, nombre et viabilité des spermatozoïdes. Les résultats montrent une augmentation significative du poids et de la taille

  15. Inbreeding and building up small populations of stingless bees (Hymenoptera, Apidae

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    Paulo Nogueira-Neto

    2002-12-01

    Full Text Available A study of the viability of small populations of Hymenoptera is a matter of importance to gain a better zoological, ethological, genetical and ecological knowledge of these insects, and for conservation purposes, mainly because of the consequences to the survival of colonies of many species of bees, wasps, and ants. Based on the Whiting (1943 principle, Kerr & Vencovski (1982 presented a hypothesis that states that viable populations of stingless bees (Meliponini should have at least 40 colonies to survive. This number was later extended to 44 colonies by Kerr (1985. This would be necessary to avoid any substantial amount of homozygosis in the pair of chromosomic sexual loci, by keeping at least six different sexual gene alleles in a reproductive population. In most cases this would prevent the production of useless diploid males. However, several facts weigh against considering this as a general rule. From 1990 to 2001, 287 colony divisions were made, starting with 28 foundation colonies, in the inbreeding and population experiments with the Meliponini reported here. These experiments constitute the most extensive and longest scientific research ever made with Meliponini bees. In ten different experiments presented here, seven species (one with two subspecies of Meliponini bees were inbred in five localities inside their wide-reaching native habitats, and in two localities far away from these habitats. This was done for several years. On the whole, the number of colonies increased and the loss of colonies over the years was small. In two of these experiments, although these populations were far (1,000 km and 1,200 km from their native habitat, their foundation colonies were multiplied successfuly. It was possible to build up seven strong and three expanding medium populations, starting with one, two, three or even five colonies. However, in six other cases examined here, the Whiting (1943 principle and the hypothesis of Kerr & Vencovski (1982

  16. The mitochondrial genome of the German wasp Vespula germanica (Fabricius, 1793) (Hymenoptera: Vespoidea: Vespidae).

    Science.gov (United States)

    Zhou, Yuan; Hu, Yu-Lin; Xu, Zai-Fu; Wei, Shu-Jun

    2016-07-01

    The mitochondrial genome of the German wasp Vespula germanica (Fabricius, 1793) (Hymenoptera: Vespidae) (GenBank accession no. KR703583) was sequenced in the study. It represents the first mitochondrial genome from the genus Vespula. There are totally 163 42 bp in the currently sequenced portion of the genome, containing 13 protein-coding, two rRNA, and 18 tRNA genes and a partial A + T-rich region. Four tRNA genes of trnI, trnQ, trnM and trnY located at the downstream of the A + T-rich region were failed to sequence. At least two rearrangement events occurred in the sequenced region compared with the pupative ancestral arrangement of insects, corresponding to the translocation or remote inversion of tnnY from trnW-trnC-trnY cluster to the region of trnI-trnQ-trnM cluster and translocation of trnL1 from the downstream to the upstream of nad1 gene. All protein-coding genes start with ATN codons. Twelve and one protein-coding genes stop with termination codon TAA and T, respectively. Phylogenetic analysis using the Bayesian method based on all codon positions of the 13 protein-coding genes supports the monophyly of Vespidae and Formicidae. Within the Formicidae, the Myrmicinae and Formicinae form a sister group and then sister to the Dolichoderinae, while within the Vespidae, the Eumeninae sister to the lineage of Vespinae + Polistinae.

  17. Phylogeography of the Wheat Stem Sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae): Implications for Pest Management.

    Science.gov (United States)

    Lesieur, Vincent; Martin, Jean-François; Weaver, David K; Hoelmer, Kim A; Smith, David R; Morrill, Wendell L; Kadiri, Nassera; Peairs, Frank B; Cockrell, Darren M; Randolph, Terri L; Waters, Debra K; Bon, Marie-Claude

    2016-01-01

    The wheat stem sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae), is a key pest of wheat in the northern Great Plains of North America, and damage resulting from this species has recently expanded southward. Current pest management practices are inadequate and uncertainty regarding geographic origin, as well as limited data on population structure and dynamics across North America impede progress towards more informed management. We examined the genetic divergence between samples collected in North America and northeastern Asia, the assumed native range of C. cinctus using two mitochondrial regions (COI and 16S). Subsequently, we characterized the structure of genetic diversity in the main wheat producing areas in North America using a combination of mtDNA marker and microsatellites in samples collected both in wheat fields and in grasses in wildlands. The strong genetic divergence observed between North American samples and Asian congeners, in particular the synonimized C. hyalinatus, did not support the hypothesis of a recent American colonization by C. cinctus. Furthermore, the relatively high genetic diversity both with mtDNA and microsatellite markers offered additional evidence in favor of the native American origin of this pest. The genetic diversity of North American populations is structured into three genetic clusters and these are highly correlated with geography. Regarding the recent southern outbreaks in North America, the results tend to exclude the hypothesis of recent movement of damaging wheat stem sawfly populations from the northern area. The shift in host plant use by local populations appears to be the most likely scenario. Finally, the significance of these findings is discussed in the context of pest management.

  18. DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini).

    Science.gov (United States)

    Rocha, Marla Piumbini; Pompolo, Silvia Das Graças; Dergam, Jorge Abdala; Fernandes, Anderson; Campos, Lucio Antonio De Oliveira

    2002-01-01

    We analyzed patterns of heterochromatic bands in the Neotropical stingless bee genus Melipona (Hymenoptera, Meliponini). Group I species (Melipona bicolor bicolor, Melipona quadrifasciata, Melipona asilvae, Melipona marginata, Melipona subnitida) were characterized by low heterochromatic content. Group II species (Melipona capixaba, Melipona compressipes, Melipona crinita, Melipona seminigra fuscopilosa e Melipona scutellaris) had high heterochromatic content. All species had 2n = 18 and n = 9. In species of Group I heterochromatin was pericentromeric and located on the short arm of acrocentric chromosomes, while in Group II species heterochromatin was distributed along most of the chromosome length. The most effective sequential staining was quinacrine mustard (QM)/distamycin (DA)/chromomycin A3(CMA3)/4-6-diamidino-2-phenylindole (DAPI). Heterochromatic and euchromatic bands varied extensively within Group I. In Group II species euchromatin was restricted to the chromosome tips and it was uniformly GC+. Patterns of restriction enzymes (EcoRI, DraI, HindIII) showed that heterochromatin was heterogeneous. In all species the first pair of homologues was of unequal size and showed heteromorphism of a GC+ pericentromeric heterochromatin. In M. asilvae (Group I) this pair bore NOR and in M. compressipes (Group II) it hybridized with a rDNA FISH probe. As for Group I species the second pair was AT+ in M. subnitida and neutral for AT and GC in the remaining species of this group. Outgroup comparison indicates that high levels of heterochromatin represent a derived condition within Melipona. The pattern of karyotypic evolution sets Melipona in an isolated position within the Meliponini.

  19. Larval development of Physocephala (Diptera, Conopidae in the bumble bee Bombus morio (Hymenoptera, Apidae

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    Fábio C Abdalla

    2014-12-01

    Full Text Available Larval development of Physocephala (Diptera, Conopidae in the bumble bee Bombus morio (Hymenoptera, Apidae. In the summer of 2012, a high incidence of conopid larvae was observed in a sample of female B. morio collected in remaining fragments of semidecidual forest and Cerrado, in the municipality of Sorocaba, state of São Paulo, Brazil. The larval development of conopid flies was studied, beginning at the larval instars (LO to L3 and PUP, until the emergence of the imago under laboratory conditions and inside the host. At the first instar, or LO, the microtype larvae measured less than 1 mm in length. During the transition from L1 to L3, the larvae grew in length. At L3, the larvae doubled their length (4 mm and then started to develop both in length and width, reaching the PUP stage with 10 mm in length and 7 mm in width. The main characteristic that differentiates L3 from the early instars is the larger body size and the beginning of posterior spiracle development. The development from PUP to puparium took less than 24h. The bees died ten days after the fly oviposition, or just before full PUP development. The early development stages (egg-LO to L1 were critical for larva survival. The pupa was visible between the intersegmental sternites and, 32 days after pupation, a female imago of Physocephala sp. emerged from one bee. The puparium and the fly measured approximately 10 mm in length. In a single day of collection, up to 45% of the bumble bees collected were parasitized by conopid flies.

  20. Evolution of Metapostnotum in Flat Wasps (Hymenoptera, Bethylidae: Implications for Homology Assessments in Chrysidoidea.

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    Ricardo Kawada

    Full Text Available Some authors in the past based their conclusions about the limits of the metapostnotum of Chrysidoidea based on the position of the mesophragmo-metaphragmal muscle, rather than aspects of the skeleton and musculature associated with the metapectal-propodeal complex. The latter character system suggests another interpretation of the metapostnotum delimitation. Given this scenario, the main goal of this work is to present a new perspective on the metapostnotum in Chrysidoidea, especially Bethylidae, helping to resolve questions related to the evolution of the metapostnotum. This is based on homologies established by associating of insertion points of ph2-ph3 and ph3-T2 muscles with the delimitation of the respective sclerite the muscles insert into. Our results indicate that, according the position of the metaphragmal muscles, the metapostnotum in Bethylidae is medially expanded in the propodeal disc and has different forms of configuration. Internally, the limits of the metapostnotum can be tracked by the shape of the mesopostnotum, and vice versa. Thus, the anteromedian area of the propodeal disc sensu Evans was reinterpreted in the current study as the metapostnotum. In conjunction with associated structures, we provide evidence to clarify the relationships between the families within Chrysidoidea, although certain families like Embolemidae, Dryinidae and Chrysididae exhibit extreme modifications of the condition found in Aculeata, as observed in Bethylidae. We review the terminology used to describe anatomical features on the metapectal-propodeal complex in Bethylidae in general, and provide a list of recommended terms in accordance with the online Hymenoptera Anatomy Ontology. The morphology of the studied subfamilies are illustrated. Studies that focus on a single structure, across a larger number of taxa, are more insightful and present specific questions that can contribute to broader issues, thus providing a better understanding of the

  1. Application of recombinant antigen 5 allergens from seven allergy-relevant Hymenoptera species in diagnostics.

    Science.gov (United States)

    Schiener, M; Eberlein, B; Moreno-Aguilar, C; Pietsch, G; Serrano, P; McIntyre, M; Schwarze, L; Russkamp, D; Biedermann, T; Spillner, E; Darsow, U; Ollert, M; Schmidt-Weber, C B; Blank, S

    2017-01-01

    Hymenoptera stings can cause severe anaphylaxis in untreated venom-allergic patients. A correct diagnosis regarding the relevant species for immunotherapy is often hampered by clinically irrelevant cross-reactivity. In vespid venom allergy, cross-reactivity between venoms of different species can be a diagnostic challenge. To address immunological IgE cross-reactivity on molecular level, seven recombinant antigens 5 of the most important Vespoidea groups were assessed by different diagnostic setups. The antigens 5 of yellow jackets, hornets, European and American paper wasps, fire ants, white-faced hornets, and Polybia wasps were recombinantly produced in insect cells, immunologically and structurally characterized, and their sIgE reactivity assessed by ImmunoCAP, ELISA, cross-inhibition, and basophil activation test (BAT) in patients with yellow jacket or Polistes venom allergy of two European geographical areas. All recombinant allergens were correctly folded and structural models and patient reactivity profiles suggested the presence of conserved and unique B-cell epitopes. All antigens 5 showed extensive cross-reactivity in sIgE analyses, inhibition assays, and BAT. This cross-reactivity was more pronounced in ImmunoCAP measurements with venom extracts than in sIgE analyses with recombinant antigens 5. Dose-response curves with the allergens in BAT allowed a differentiated individual dissection of relevant sensitization. Due to extensive cross-reactivity in various diagnostic settings, antigens 5 are inappropriate markers for differential sIgE diagnostics in vespid venom allergy. However, the newly available antigens 5 from further vespid species and the combination of recombinant allergen-based sIgE measurements with BAT represents a practicable way to diagnose clinically relevant sensitization in vespid venom allergy. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  2. Natural history of Hymenoptera venom allergy in children not treated with immunotherapy.

    Science.gov (United States)

    Lange, Joanna; Cichocka-Jarosz, Ewa; Marczak, Honorata; Krauze, Agnieszka; Tarczoń, Izabela; Świebocka, Ewa; Lis, Grzegorz; Brzyski, Piotr; Nowak-Węgrzyn, Anna

    2016-03-01

    Differences in treatment approach still exist for children after systemic sting reactions. In addition, there are still some doubts about when systemic reactors should be treated with venom immunotherapy (VIT). To determine the rate of sting recurrence and natural history of Hymenoptera venom allergy (HVA) in children not treated with VIT. A total of 219 children diagnosed as having HVA who were not treated with VIT were identified in 3 pediatric allergology centers. Survey by telephone or mail with the use of a standardized questionnaire was conducted. The number of field re-stings, subsequent symptoms, and provided treatment were analyzed. A total of 130 of the 219 patients responded to the survey, for a response rate of 59.4%. During the median follow-up period of 72 months (interquartile range, 52-85 months), 44 children (77% boys) were stung 62 times. Normal reactions were most common, occurring in 27 patients (62%). Severe systemic reactions (SSRs) occurred in 8 (18%) of those who were re-stung. The subsequent reaction was significantly milder (P insect (P insect (P = .03). In children with SSRs, median time from diagnosis to re-sting was 2 times longer than that in those with large local reactions and normal reactions (P = .007). Most children with HVA not treated with VIT reported milder reactions after a re-sting. Probability of SSR to re-sting increases along with the severity of initial reaction. Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  3. Selectivity of organic compounds to the egg parasitoid Telenomus remus Nixon (Hymenoptera: Plastygastridae

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    Débora Mello da Silva

    2016-02-01

    Full Text Available The selectivity of insecticides, bio-protective mixtures, and biofertilizers used in organic soybean production was evaluated for adults and pupae of the egg parasitoid Telenomus remus Nixon (Hymenoptera: Platygastridae under laboratory conditions in accordance with protocols proposed by the International Organization for Biological Control (IOBC. The products sprayed (dosage/180L of water were: 1 Baculovírus anticarsia 140x109 cpi; 2 Bacillus thuringiensis 16.8g; 3 Azadirachtin-A, azadirachtin-B, nimbina and salamina 9.6 ppm; 4 Rotenoids 4 liters; 5 Nitrogen 1.3%, phosphorus 3.0% and total organic carbon 8.0% 3 liters; 6 Sodium silicate 2% 4 liters; 7 Copper 7% + calcium 3.3% 1.8 liters; 8 Sulfur 20% + quicklime 10% 1.8 liters; 9 Chlorpyrifos 384g (positive control; 10 Distilled H2O (negative control. The results of experiments using pupae indicate that the organic compounds were classified as harmless (Class 1, except for the copper 7% + calcium 3.3% and sulfur 20% + quicklime 10%, which were classified as slightly harmful (Class 2. The contact bioassay with adults showed that all products were classified as harmless (Class 1. Only chlorpyrifos (384g was classified as harmful (Class 4 for both stages of the parasitoid. However, the use of this product (chlorpyrifos is not permitted in organic farming, and even in conventional farming is recommended, where feasible, replacement of the product with one compatible with the preservation of T. remus in nature. Thus, the products tested and used in organic soybean production were considered compatible with the parasitoid eggs of T. remus.

  4. Thermal hygrometric requirements for the rearing and release of Tamarixia radiata (Waterston (Hymenoptera, Eulophidae

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    Mariuxi Lorena Gómez-Torres

    2014-09-01

    Full Text Available Thermal hygrometric requirements for the rearing and release of Tamarixia radiata (Waterston (Hymenoptera, Eulophidae. Tamarixia radiata is the main agent for the biological control of Diaphorina citri in Brazil with a parasitism rate ranging from 20 to 80%. This study investigated the influence of temperature on the development, fecundity and longevity of adults of T. radiata and the effect of relative humidity (RH on their parasitism capacity and survival rate in the pre-imaginal period. The effect of temperature was assessed in the range between 15 and 35 ± 1ºC, 70 ± 10% RH, and a 14-h photophase. The RH effect was evaluated in the range from 30 to 90 ± 10%, temperature at 25 ± 1ºC, and photophase of 14-h. At 25ºC, circa 166.7 nymphs were parasitized, the highest parasitism capacity observed compared to other treatments. The longest longevity of females was observed at 25ºC, although the rate did not differ in the 20-30ºC temperature range. The threshold temperature (TT was 7.2ºC, and 188.7 degrees-day were required for the development (egg-to-adult period. The parasitism rate and longevity were higher at 50 and 70% of RH. This shows that temperature and RH may affect the parasitism capacity of T. radiata on nymphs of D. citri, which can explain the great parasitism variation for D. citri observed in citrus groves in São Paulo State, Brazil.

  5. An evaluation of phylogenetic informativeness profiles and the molecular phylogeny of diplazontinae (Hymenoptera, Ichneumonidae).

    Science.gov (United States)

    Klopfstein, Seraina; Kropf, Christian; Quicke, Donald L J

    2010-03-01

    How to quantify the phylogenetic information content of a data set is a longstanding question in phylogenetics, influencing both the assessment of data quality in completed studies and the planning of future phylogenetic projects. Recently, a method has been developed that profiles the phylogenetic informativeness (PI) of a data set through time by linking its site-specific rates of change to its power to resolve relationships at different timescales. Here, we evaluate the performance of this method in the case of 2 standard genetic markers for phylogenetic reconstruction, 28S ribosomal RNA and cytochrome oxidase subunit 1 (CO1) mitochondrial DNA, with maximum parsimony, maximum likelihood, and Bayesian analyses of relationships within a group of parasitoid wasps (Hymenoptera: Ichneumonidae, Diplazontinae). Retrieving PI profiles of the 2 genes from our own and from 3 additional data sets, we find that the method repeatedly overestimates the performance of the more quickly evolving CO1 compared with 28S. We explore possible reasons for this bias, including phylogenetic uncertainty, violation of the molecular clock assumption, model misspecification, and nonstationary nucleotide composition. As none of these provides a sufficient explanation of the observed discrepancy, we use simulated data sets, based on an idealized setting, to show that the optimum evolutionary rate decreases with increasing number of taxa. We suggest that this relationship could explain why the formula derived from the 4-taxon case overrates the performance of higher versus lower rates of evolution in our case and that caution should be taken when the method is applied to data sets including more than 4 taxa.

  6. Monitoring hymenoptera and diptera pollinators in a sub-tropical forest of southern punjab, pakistan

    International Nuclear Information System (INIS)

    Bashir, M.; Sajjad, A.

    2013-01-01

    Bees (Hymenoptera) and flies (Diptera) play an essential role in natural and agricultural ecosystems as pollinators of flowering plants while pollinators are declining around the world. Colored pan traps and Malaise traps have widely been used for monitoring pollinators. However, their efficiencies may vary with landscapes and type of fauna in a particular habitat. A yearlong study was carried out during 2009 to investigate the relative efficacy of colored pan traps and Malaise traps towards sampling flies and bees for the first time in a sub-tropical wildlife sanctuary Pirowal of Southern Punjab, Pakistan. Fifteen pan traps (5 each of 3 colors i.e. white, red and blue) were deployed against one Malaise trap for 7 hours (9:00-16:00 hrs) on fortnightly basis. For the comparison and confirmation of an insect as a floral visitor, collection with the hand net was also performed. It was concluded that hand net collection is essential to have a comprehensive list of floral visitors of an area as the maximum number (63) of species and their abundance (5428 individuals) were recorded with it. Malaise trap collected only 671 individuals of 48 species. Although blue, yellow and white pan traps caught 46, 51 and 35 species but the numbers of individuals (1383) were fairly higher than that of Malaise traps. Keeping in view the cost effectiveness and better performance of colored pan traps, we recommend species specific pan trap colors when targeting certain groups or species, nevertheless variety of pan colors should be used when sampling overall biodiversity. We generalize these findings for both bees and flies due to similar collection pattern i.e. the maximum abundance and diversity in hand net method followed by pan traps and Malaise traps. (author)

  7. Toxicity of some insecticides used in maize crop on Trichogramma pretiosum (Hymenoptera, Trichogrammatidae immature stages

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    Jander R Souza

    2014-04-01

    Full Text Available Fall armyworm Spodoptera frugiperda (J.E. Smith, 1797 (Lepidoptera: Noctuidae is an important pest of maize (Zea mays L. crops in Brazil. The effects of beta-cypermethrin, chlorfenapyr, chlorpyrifos, spinosad, etofenprox, triflumuron, alpha-cypermethrin/teflubenzuron, and lambda-cyhalothrin/thiamethoxam on Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae immature stages were evaluated. Eggs of Anagasta kuehniella (Zeller, 1879 (Lepidoptera: Pyralidae, containing immature stages of the parasitoid were dipped in water solution pesticides, to evaluate their effects on emergence and sex ratio of F1 parasitoids. For F2 parasitoids, emergence, parasitism capacity, and sex ratio were evaluated. Beta-cypermethrin, chlorfenapyr, chlorpyrifos, and spinosad affected the emergence success of F1 T. pretiosum. Insects exposed to etofenprox and alpha-cypermethrin/teflubenzuron during the egg-larval period and to lambda-cyhalothrin/thiamethoxam during the pupal stage also suffered reduction in the emergence. Beta-cypermethrin affected the sex ratio of F1 T. pretiosum from host eggs treated during the egg-larval period; spinosad affected it during the egg-larval period and the pupal stage, whereas chlorpyrifos did the same when applied during the pupal stage. Chlorpyrifos also affected the sex ratio of F2 parasitoids, but only when applied during the egg-larval period, whereas chlorfenapyr reduced this trait when applied during the pre-pupal phase. Chlorpyrifos and alpha-cypermethrin/teflubenzuron affected the parasitism capacity of F1 females from eggs treated during the egg-larval period. Considering the overall effects, only etofenprox and triflumuron were selective on T. pretiosum when applied on parasitized A. kuehniella eggs. Further studies need to be carried out to verify the toxicity of the other pesticides under semi-field and field conditions.

  8. Side-Effects of Glyphosate to the Parasitoid Telenomus remus Nixon (Hymenoptera: Platygastridae).

    Science.gov (United States)

    Stecca, C S; Bueno, A F; Pasini, A; Silva, D M; Andrade, K; Filho, D M Z

    2016-04-01

    The aim of this study was to compare the side-effects of glyphosate to the parasitoid Telenomus remus Nixon (Hymenoptera: Platygastridae) when parasitoids were exposed to this chemical at the pupal (inside host eggs) and adult stages. Bioassays were conducted under laboratory conditions according to the International Organization for Biological Control (IOBC) standard methods for testing side-effects of pesticides to egg parasitoids. Different glyphosate-based pesticides (Roundup Original®, Roundup Ready®, Roundup Transorb®, Roundup WG®, and Zapp Qi®) were tested at the same acid equivalent concentration. Treatments were classified following the IOBC toxicity categories as (1) harmless, (2) slightly harmful, (3) moderately harmful, and (4) harmful. When tested against T. remus adults, Roundup Original®, Roundup Ready®, Roundup Transorb®, and Roundup WG® reduced parasitism 2 days after parasitoid emergence, being classified as slightly harmful. Differently, when tested against T. remus pupae, all tested glyphosate-based products did not differ in their lethal effect and therefore did not reduce T. remus adult emergence or parasitism capacity, being classified as harmless. However, differences on sublethal toxicity were found. Parasitism of individuals emerging from parasitized eggs sprayed at the pupal stage of T. remus with Zapp Qi® was lower compared to control, but parasitism was still higher than 66%, and therefore, Zapp Qi® was still classified as harmless. In conclusion, all tested glyphosate-based products can be used in agriculture without negative impact to T. remus as none was classified as harmful or moderately harmful to this parasitoid when exposure occurred at the pupal or adult stages.

  9. Biology of Blepyrus clavicornis (Compere (Hymenoptera: Encyrtidae, a parasitoid of Pseudococcus viburni (Signoret (Hemiptera: Pseudococcidae

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    Vitor Pacheco da Silva

    Full Text Available Abstract Encyrtids (Hymenoptera: Encyrtidae are the most important and diverse group of natural enemies of mealybugs (Hemiptera: Pseudococcidae. Blepyrus clavicornis (Compere is the most common parasitoid associated with Pseudococcus viburni (Signoret in the Serra Gaúcha region, Brazil. We conducted laboratory studies to assess the development time, sex ratio, adult longevity, host stage selection for parasitism, and effect of food on the longevity of adult females of B. clavicornis. The experiments were conducted in a climate chamber at 25 ± 1 °C, 70 ± 10% RH and 12:12 L:D photoperiod. The solitary parasitoid B. clavicornis parasitized third-instar and adult female stages of P. viburni. The development time was more than 30 days (31.75 ± 0.38 for females and 30.02 ± 0.34 for males when B. clavicornis laid eggs in adult mealybug females, and 35 days (36.50 ± 0.50 for females and 34.24 ± 0.43 for males on third-instar mealybug nymphs. The wasps did not survive longer than four days when they were fed only water, while females survived for about 30 days when fed with honey. The lifespan of females is about 20 days longer than the lifespan of males. Although B. clavicornis can provide significant natural control, reducing the number of individuals in the next generation by parasitizing advanced mealybug instars, we consider it unpromising for use in applied biological-control programs. Furthermore, the predominance of males in the progeny observed here suggests that P. viburni may not be the most suitable or preferred host for B. clavicornis.

  10. Poles Apart: Comparing Trends of Alien Hymenoptera in New Zealand with Europe (DAISIE.

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    Darren Ward

    Full Text Available Developing generalisations of invasive species is an important part of invasion biology. However, trends and generalisations from one part of the world may not necessarily hold elsewhere. We present the first inventory and analysis of all Hymenoptera alien to New Zealand, and compare patterns from New Zealand with those previously published from Europe (DAISIE. Between the two regions there was broad correlation between families with the highest number of alien species (Braconidae, Encyrtidae, Pteromalidae, Eulophidae, Formicidae, Aphelinidae. However, major differences also existed. The number of species alien to New Zealand is higher than for Europe (334 vs 286, and major differences include: i the much lower proportion of intentionally released species in New Zealand (21% vs 63% in Europe; and ii the greater proportion of unintentionally introduced parasitoids in New Zealand (71.2% vs 22.6%. The disharmonic 'island' nature of New Zealand is shown, as a high proportion of families (36% have no native representatives, and alien species also represent >10% of the native fauna for many other families. A much larger proportion of alien species are found in urban areas in New Zealand (60% compared to Europe (~30%, and higher numbers of alien species were present earlier in New Zealand (especially <1950. Differences in the origins of alien species were also apparent. Unlike Europe, the New Zealand data reveals a change in the origins of alien species over time, with an increasing dominance of alien species from Australasia (a regional neighbour during the past 25 years. We recommend that further effort be made towards the formation, and analysis, of regional inventories of alien species. This will allow a wider range of taxa and regions to be examined for generalisations, and help assess and prioritise the risk posed by certain taxa towards the economy or environment.

  11. Characterization of Resistance to Cephus cinctus (Hymenoptera: Cephidae) in Barley Germplasm.

    Science.gov (United States)

    Varella, Andrea C; Talbert, Luther E; Achhami, Buddhi B; Blake, Nancy K; Hofland, Megan L; Sherman, Jamie D; Lamb, Peggy F; Reddy, Gadi V P; Weaver, David K

    2018-04-02

    Most barley cultivars have some degree of resistance to the wheat stem sawfly (WSS), Cephus cinctus Norton (Hymenoptera: Cephidae). Damage caused by WSS is currently observed in fields of barley grown in the Northern Great Plains, but the impact of WSS damage among cultivars due to genetic differences within the barley germplasm is not known. Specifically, little is known about the mechanisms underlying WSS resistance in barley. We characterized WSS resistance in a subset of the spring barley CAP (Coordinated Agricultural Project) germplasm panel containing 193 current and historically important breeding lines from six North American breeding programs. Panel lines were grown in WSS infested fields for two consecutive years. Lines were characterized for stem solidness, stem cutting, WSS infestation (antixenosis), larval mortality (antibiosis), and parasitism (indirect plant defense). Variation in resistance to WSS in barley was compared to observations made for solid-stemmed resistant and hollow-stemmed susceptible wheat lines. Results indicate that both antibiosis and antixenosis are involved in the resistance of barley to the WSS, but antibiosis seems to be more prevalent. Almost all of the barley lines had greater larval mortality than the hollow-stemmed wheat lines, and only a few barley lines had mortality as low as that observed in the solid-stemmed wheat line. Since barley lines lack solid stems, it is apparent that barley has a different form of antibiosis. Our results provide information for use of barley in rotation to control the WSS and may provide a basis for identification of new approaches for improving WSS resistance in wheat.

  12. Effects of neonicotinoid imidacloprid exposure on bumble bee (Hymenoptera: Apidae) queen survival and nest initiation.

    Science.gov (United States)

    Wu-Smart, Judy; Spivak, Marla

    2018-02-08

    Neonicotinoids are highly toxic to insects and may systemically translocate to nectar and pollen of plants where foraging bees may become exposed. Exposure to neonicotinoids can induce detrimental sublethal effects on individual and colonies of bees and may have long-term impacts, such as impaired foraging, reduced longevity, and reduced brood care or production. Less well-studied are the potential effects on queen bumble bees that may become exposed while foraging in the spring during colony initiation. This study assessed queen survival and nest founding in caged bumble bees [Bombus impatiens (Cresson) (Hymenoptera: Apidae)] after chronic (18-d) dietary exposure of imidacloprid in syrup (1, 5, 10, and 25 ppb) and pollen (0.3, 1.7, 3.3, and 8.3 ppb), paired respectively. Here we show some mortality in queens exposed at all doses even as low as 1 ppb, and, compared with untreated queens, significantly reduced survival of treated queens at the two highest doses. Queens that survived initial imidacloprid exposure commenced nest initiation; however, they exhibited dose-dependent delay in egg-laying and emergence of worker brood. Furthermore, imidacloprid treatment affected other parameters such as nest and queen weight. This study is the first to show direct impacts of imidacloprid at field-relevant levels on individual B. impatiens queen survival and nest founding, indicating that bumble bee queens are particularly sensitive to neonicotinoids when directly exposed. This study also helps focus pesticide risk mitigation efforts and highlights the importance of reducing exposure rates in the early spring when bumble bee queens, and other wild bees are foraging and initiating nests. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  13. Phenotypic Variation in Fitness Traits of a Managed Solitary Bee, Osmia ribifloris (Hymenoptera: Megachilidae).

    Science.gov (United States)

    Sampson, B J; Rinehart, T A; Kirker, G T; Stringer, S J; Werle, C T

    2015-12-01

    We investigated fitness in natural populations of a managed solitary bee Osmia ribifloris Cockerell (Hymenoptera: Megachilidae) from sites separated from 400 to 2,700 km. Parental wild bees originated in central Texas (TX), central-northern Utah (UT), and central California (CA). They were then intercrossed and raised inside a mesh enclosure in southern Mississippi (MS). Females from all possible mated pairs of O. ribifloris produced F1 broods with 30-40% female cocoons and outcrossed progeny were 30% heavier. Mitochondrial (COI) genomes of the four populations revealed three distinct clades, a TX-CA clade, a UT clade, and an MS clade, the latter (MS) representing captive progeny of CA and UT bees. Although classified as separate subspecies, TX and CA populations from 30° N to 38° N latitude shared 98% similarity in COI genomes and the greatest brood biomass per nest straw (600- to 700-mg brood). Thus, TX and CA bees show greater adaptation for southern U.S. sites. In contrast, UT-sourced bees were more distantly related to TX and CA bees and also produced ∼50% fewer brood. These results, taken together, confirm that adult O. ribifloris from all trap-nest sites are genetically compatible, but some phenotypic variation exists that could affect this species performance as a commercial blueberry pollinator. Males, their sperm, or perhaps a substance in their sperm helped stabilize our captive bee population by promoting legitimate nesting over nest usurpation. Otherwise, without insemination, 50% fewer females nested (they nested 14 d late) and 20% usurped nests, killing 33-67% of brood in affected nests. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  14. Acute toxicities and sublethal effects of some conventional insecticides on Trichogramma chilonis (Hymenoptera: Trichogrammatidae).

    Science.gov (United States)

    Wang, De-Sen; He, Yu-Rong; Guo, Xiang-Ling; Luo, Yong-Li

    2012-08-01

    The acute toxicity of 10 conventional insecticides to adult of Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) was bioassayed by membrane method, and then their sublethal effects on the parasitoid were evaluated in the laboratory. Based on sublethal concentration (LC30) values at 8 h after treatment, we determined that adult T. chilonis were the most susceptible to chlorfenapyr, followed by fipronil, spinosad, avermectins, beta-cypermethrin, and cartap, with lethal concentration (LC)30 values of 0.3133, 0.3269, 1.5408, 3.2961, 6.1469, and 9.021 mg/liter, respectively. The field-recommended concentrations of chlorfluazuron, indoxacarb, Bacillus thuringiensis, and tebufenozide caused Cartap and spinosad also reduced longevity (8 and 7.9 d) and fecundity (110.77 and 117.2) of treated adults, but cartap enhanced the female percentage of F1 offspring (61.6%), resulting a statistical higher R0, r(m), and lambda of treated T. chilonis. In contrast, chlorfluazuron and tebufenozide increased longevity (16.4 and 15.4 d) and fecundity (248 and 256.9) of treated adults but slightly decreased the female percentage of F1 offspring (31.4 and 38.1%). Although chlorfenapyr showed no adverse influence on longevity and fecundity, it remarkably reduced the female percentage of F1 offspring (13.5%), leading to a lower R0, r(m), and lambda of treated T. chilonis. Indoxacarb, B. thuringiensis, and beta-cypermethrin had no obvious sublethal effects on the longevity and fecundity of treated adults. Based on these results, we consider B. thuringienesis, chlorfluazuron, indoxacarb, beta-cypermethrin, and tebufenozide safe to T. chilonis, suggesting that these insecticides are compatible with this parasitoid when being used in the field. However, fipronil, chlorfenapyr, spinosad, and avermectins were very harmful to T. chilonis. Timing of application of these insecticides was critical.

  15. Seletividade de herbicidas registrados para a cultura do milho a adultos de Trichogramma pretiosum (Hymenoptera: Trichogrammatidae Selectivity of herbicides registered on corn to Trichogramma pretiosum (Hymenoptera: Trichogrammatidae

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    G.J. Stefanello Júnior

    2008-06-01

    Full Text Available A seletividade de 24 herbicidas registrados para a cultura do milho foi avaliada a Trichogramma pretiosum em condições de laboratório (temperatura de 25±1 ºC, umidade relativa de 70±10%, fotofase de 14 horas e luminosidade de 500 lux. Adultos de T. pretiosum foram colocados em contato com uma película seca dos herbicidas pulverizados sobre placas de vidro e avaliou-se a capacidade de parasitismo das fêmeas. A redução na capacidade de parasitismo dos tratamentos foi comparada com a da testemunha (água destilada e utilizada para classificar os herbicidas em 1, inócuo (99%. Os herbicidas Callisto, Equip Plus, Extrazin SC, Primóleo, Provence 750 WG e Siptran 500 SC são inócuos (classe 1; Agrisato 480 SL, Gesaprim GrDA, Glifos, Glyphosate Nortox, Gliz 480 SL, Polaris, Primatop SC, Sanson 40 SC, Trop e Zapp Qi, levemente nocivos (classe 2; Finale, Herbadox, Poast, Roundup Original, Roundup Transorb e Roundup WG, moderadamente nocivos (classe 3; e Gramoxone 200 e Primestra Gold, nocivos (classe 4 aos adultos de T. pretiosum, nas dosagens utilizadas. Os herbicidas nocivos (classes 2, 3 e 4 deverão passar para as etapas seguintes, que envolverão testes sobre as fases imaturas do parasitóide em condições de laboratório e adultos a campo.The selectivity of 24 herbicides registered on corn to Trichogramma pretiosum (Hymenoptera: Trichogrammatidae was assessed under laboratory conditions (25 ± 1 ºC temperature, 70 ± 10% relative humidity, 14 4 photophase and brightness 500 lux. The adult parasitoids were submitted to a dry film of the herbicides applied on glass plates and the parasitism capacity of the females was evaluated. Reduced parasitism capacity in the treatments was compared with the negative control (distilled water and used to classify the herbicides into four categories: 1, harmless ( 99%. The herbicides Callisto, Equip Plus, Extrazin SC, Primóleo, Provence 750 WG and Siptran 500 SC were found to be harmless (class 1

  16. Genotyping-by-sequencing provides the first well-resolved phylogeny for coffee (Coffea) and insights into the evolution of caffeine content in its species: GBS coffee phylogeny and the evolution of caffeine content.

    Science.gov (United States)

    Hamon, Perla; Grover, Corrinne E; Davis, Aaron P; Rakotomalala, Jean-Jacques; Raharimalala, Nathalie E; Albert, Victor A; Sreenath, Hosahalli L; Stoffelen, Piet; Mitchell, Sharon E; Couturon, Emmanuel; Hamon, Serge; de Kochko, Alexandre; Crouzillat, Dominique; Rigoreau, Michel; Sumirat, Ucu; Akaffou, Sélastique; Guyot, Romain

    2017-04-01

    A comprehensive and meaningful phylogenetic hypothesis for the commercially important coffee genus (Coffea) has long been a key objective for coffee researchers. For molecular studies, progress has been limited by low levels of sequence divergence, leading to insufficient topological resolution and statistical support in phylogenetic trees, particularly for the major lineages and for the numerous species occurring in Madagascar. We report here the first almost fully resolved, broadly sampled phylogenetic hypothesis for coffee, the result of combining genotyping-by-sequencing (GBS) technology with a newly developed, lab-based workflow to integrate short read next-generation sequencing for low numbers of additional samples. Biogeographic patterns indicate either Africa or Asia (or possibly the Arabian Peninsula) as the most likely ancestral locality for the origin of the coffee genus, with independent radiations across Africa, Asia, and the Western Indian Ocean Islands (including Madagascar and Mauritius). The evolution of caffeine, an important trait for commerce and society, was evaluated in light of our phylogeny. High and consistent caffeine content is found only in species from the equatorial, fully humid environments of West and Central Africa, possibly as an adaptive response to increased levels of pest predation. Moderate caffeine production, however, evolved at least one additional time recently (between 2 and 4Mya) in a Madagascan lineage, which suggests that either the biosynthetic pathway was already in place during the early evolutionary history of coffee, or that caffeine synthesis within the genus is subject to convergent evolution, as is also the case for caffeine synthesis in coffee versus tea and chocolate. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Leaf-litter amount as a factor in the structure of a ponerine ants community (Hymenoptera, Formicidae, Ponerinae in an eastern Amazonian rainforest, Brazil

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    Alexandro Herbert dos Santos Bastos

    2011-12-01

    Full Text Available Leaf-litter amount as a factor in the structure of a ponerine ants community (Hymenoptera, Formicidae, Ponerinae in an eastern Amazonian rainforest, Brazil. Leaf-litter may be an important factor in structuring ponerine ant communities (Hymenoptera, Formicidae, Ponerinae in tropical rainforests. We specifically examined how leaf-litter affects the structure of a ponerine ant community in primary Amazonian rainforest sites at the Ferreira Penna Scientific Station, Pará, Brazil. A total of 53 species belonging to eight genera of three ponerine tribes were collected with mini-Winkler extractors. The amount of leaf-litter positively affected the abundance and richness of the ponerine ant community, and also influenced species composition. Nearby samples often had low species similarity, especially when adjacent samples differed in the amount of leaf-litter. Leaf-litter availability in Amazonian primary forests is a key factor for distribution of ground-dwelling ponerine species, even at small scales.

  18. Egg parasitoids (Hymenoptera: Mymaridae and Trichogrammatidae) of the gall-making leafhopper Scenergates viridis (Hemiptera: Cicadellidae) from Uzbekistan, with taxonomic notes on the Palaearctic species of Aphelinoidea.

    Science.gov (United States)

    Rakitov, Roman; Triapitsyn, Serguei V

    2013-01-01

    A new species of the Aphelinoidea Girault (Hymenoptera: Trichogrammatidae), A. (Aphelinoidea) sariq Triapitsyn & Rakitov sp. n., is described from Uzbekistan. Both sexes were reared from eggs of the only known truly gall-making leafhopper, Scenergates viridis (Vilbaste), laid inside its galls on camelthorn, Alhagi maurorum Medikus; additional females were found dead inside the galls. Aphelinoidea sariq is the only known species of the nominate subgenus of Aphelinoidea whose body color is predominantly yellow. Taxonomic notes on other Palaearctic species of Aphelinoidea are provided; A. scythica Fursov, syn. n. is synonymized underA. (Aphelinoidea) turanica S. Trjapitzin. Another trichogrammatid, Par-acentrobia (Paracentrobia) sp., was reared from eggs of S. viridis in much smaller numbers. Also described from the same locality and host is Gonatocerus (Lymaenon) mitjaevi Triapitsyn & Rakitov sp. n. (Hymenoptera: Mymaridae).

  19. Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae); Efeito da disponibilidade de hospedeiro e de alimento nas caracteristicas biologicas de Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae)

    Energy Technology Data Exchange (ETDEWEB)

    Pratissoli, Dirceu; Oliveira, Harley N. de; Oliveira, Regiane C. de; Zago, Hugo B. [Universidade Federal do Espirito Santo (UFES), Alegre, ES (Brazil). Centro de Ciencias Agrarias], e-mail: dirceu@npd.ufes.br, e-mail: hnoliveira@insecta.ufv.br; Vieira, Stella M.J. [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Engenharia Agricola

    2004-03-15

    Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae). Biological characteristics of Trichogramma galloi Zucchi, 1988 were evaluated in laboratory where these parasitoids were reared on eggs of Diatraea saccharalis (Lepidoptera, Pyralidae) with or without honey, and exposed to eggs of the host after 0, 6, 12, 24, 36, 48, 60, 72 and 84 hours of emergence. The parasitism rate and viability showed higher for individuals that received food. The sex ratio was not influenced by food. The number of individuals per egg only showed difference for those adults that did not receive food and stayed six hours without the host eggs. Checking the effect of the availability of eggs, only the sex ratio, with or without honey, did not show differences. The results show that T. galloi needs a carbohydrate supply and the time can influence the reproductive capacity. (author)

  20. Molecular phylogeny of Indo-Pacific carpenter ants (Hymenoptera: Formicidae, Camponotus) reveals waves of dispersal and colonization from diverse source areas

    Czech Academy of Sciences Publication Activity Database

    Clouse, R. M.; Janda, Milan; Blanchard, B.; Sharma, P.; Hoffmann, B. D.; Andersen, A. N.; Czekanski-Moir, J. E.; Krushelnycky, P.; Rabeling, C.; Wilson, E. O.; Economo, E. P.; Sarnat, E. M.; General, D. M.; Alpert, G. D.; Wheeler, W. C.

    2015-01-01

    Roč. 31, č. 4 (2015), s. 424-437 ISSN 0748-3007 R&D Projects: GA ČR GAP505/12/2467 Grant - others:Marie Curie Felloswhip(CZ) PIOFGA2009-25448 Institutional support: RVO:60077344 Keywords : Hymenoptera * Camponotus * molecular phylogeny Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.952, year: 2015 http://onlinelibrary.wiley.com/doi/10.1111/cla.12099/epdf

  1. Morphological and molecular characterization of common European species Adialytus (Hymenoptera: Braconidae: Aphidiinae) based on mtCOI barcoding gene and geometric morphometrics of the forewings

    Czech Academy of Sciences Publication Activity Database

    Stanković, S. S.; Petrović, A.; Milošević, M.I.; Starý, Petr; Kavallieratos, N. G.; Žikić, V.; Tomanović, Ž.

    2015-01-01

    Roč. 112, č. 1 (2015), s. 165-174 ISSN 1210-5759 Grant - others:Ministry of Education, Science and Technology Development of the Republic of Serbia(RS) III43001 Institutional support: RVO:60077344 Keywords : Hymenoptera * Braconidae * Adialytus Subject RIV: EG - Zoology Impact factor: 0.975, year: 2014 http://www.eje.cz/pdfs/eje/2015/01/21.pdf

  2. Revisión taxonómica del subgénero Micrandrena (Hymenoptera: Apoidea: Andrenidae: Andrena) de la Península Ibérica

    OpenAIRE

    Dardón Peralta, María José

    2011-01-01

    [ES]Las abejas (Hymenoptera: Apoidea) han sido clasificadas por diferentes autores siguiendo distintos criterios, aunque actualmente se emplea la propuesta de Michener (2007), quien establece siete familias: Andrenidae, Colletidae, Halictidae, Melittidae, Stenotritidae, Megachilidae y Apidae. La familia Andrenidae se divide en cuatro subfamilias: Oxaeinae, Andreninae, Alocandreninae y Panurginae. La subfamilia Andreninae está conformada por los géneros Ancyladrena Cockerell, 1930, Andrena...

  3. A new genus of oak gallwasp, Cyclocynips Melika, Tang & Sinclair (Hymenoptera: Cynipidae: Cynipini), with descriptions of two new species from Taiwan.

    Science.gov (United States)

    Melika, George; Tang, Chang-ti; Sinclair, Frazer; Yang, Man-miao; Lohse, Konrad; Hearn, Jack; Nicholls, James A; Stone, Graham N

    2013-01-01

    A new genus of cynipid oak gallwasp-Cyclocynips Melika, Tang, & Sinclair (Hymenoptera: Cynipidae: Cynipini), with two new species--C. uberis and C. tumorvirgae--reared from galls on oaks of the Quercus subgenus Cyclobalanopsis is described from Taiwan. Descriptions of asexual generation adults and their diagnostic characters are presented. The likelihood of yet undiscovered sexual generations and the evolution of host-plant associations in these species are discussed.

  4. A new genus and species of Eucoilinae (Hymenoptera, Cynipoidea, Figitidae parasitoid of Euxesta eluta Loew (Diptera, Otitidae attacked Bt sweet corn in Argentina

    Directory of Open Access Journals (Sweden)

    Fabiana E. Gallardo

    2017-02-01

    Full Text Available Euxestophaga Gallardo, a new genus of Eucoilinae (Hymenoptera, Cynipoidea, Figitidae and Euxestophaga argentinensis Gallardo, sp. n. from Argentina, are described and illustrated. This new genus belongs to the Ganaspini and morphologically resembles Epicoela Borgmeier and Striatovertex Schick, Forshage and Nordlander. A key to differentiate these genera is given. Specimens were reared from pupae of Euxesta eluta Loew (Diptera: Otitidae, attacked Bt sweet corn in Santa Fe province and other in Tucumán province (Argentina.

  5. Eucharitidae (Hymenoptera, Chalcidoidea, a family new to the fauna of Saudi Arabia, with the description of the previously unknown male of Eucharis (Psilogastrellus affinis Bouček

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    Neveen S. Gadallah

    2014-12-01

    Full Text Available The family Eucharitidae (Hymenoptera: Chalcidoidea is recorded for the first time for the fauna of Saudi Arabia based on Hydrorhoa caffra (Westwood and Eucharis (Psilogastrellus affinis Bouček. The record of H. caffra suggests that Al-Baha and Asir provinces should be considered as part of the Afrotropical rather than the Palaearctic region. The previously unknown male of E. affinis Bouček is described and figured. Macrophotographs of the species are provided.

  6. CEPF Western Ghats Special Series : Parasitoids (Hymenoptera of xylophagous beetles (Coleoptera attacking dead wood in southern Western Ghats, Kerala, India, with descriptions of two new species

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    P.M. Sureshan

    2013-05-01

    Full Text Available An account is given of four species of Hymenoptera parasitoids probably of the wood boring beetle Clytocera chinospila Gahan (Coleoptera: Cerambycidae from Chinnar Wildlife Sanctuary, southern Western Ghats, Kerala. Two new hymenopteran species, Eurytoma chinnarensis (Eurytomidae and Foenatopus idukkiensis (Stephanidae are described. Solenura ania Walker (Pteromalidae is reported for the first time from Kerala and Western Ghats with a new host record, and Doryctus sp. (Braconidae is reported here.

  7. Eficiência de produtos termonebulígenos no controle de Atta laevigata (Hymenoptera: Formicidae em plantio de eucalipto Efficiency of products for thermonebulization on the control of Atta laevigata (Hymenoptera: Formicidae in eucalypus plantations

    Directory of Open Access Journals (Sweden)

    Ronald Zanetti

    2008-08-01

    Full Text Available Avaliou-se a eficiência de produtos termonebulígenos, a base de clorpirifós ou de extratos vegetais, comparativamente ao uso de isca formicida, a base de sulfluramida, no controle de Atta laevigata (F. Smith, 1858 (Hymenoptera: Formicidae. Foi avaliada a percentagem de paralisação das atividades de corte de folhas e de movimentação de formigas de A. laevigata aos três, 12, 36, 63 e 86 dias após a aplicação dos tratamentos. Na última avaliação, os formigueiros foram abertos para a verificação da eficiência de controle. Todos os produtos testados apresentaram alta percentagem de paralisação das atividades de corte e de movimentação das formigas aos três e 12 dias após a aplicação, respectivamente. Os produtos a base de clorpirifós e um a base de extratos vegetais apresentaram alta eficiência no controle de A. laevigata, sendo mais efetivos que a isca formicida testada.The efficiency of products formulated with chlorpyrifos or plants extracts in thermonebuzation was evaluated and compared to the use of the granulated bait formulated with sulfluramid in the control of Atta laevigata (F. Smith, 1858 (Hymenoptera: Formicidae. The cutting activity and the movement of ants on the colonies were evaluated at three, 12, 36, 63 and 86 days after the application of the treatments. The colonies of these ants were excavated in the last evaluation to obtain the efficiency of each product. All products stopped the cutting activity and movements of the individuals of A. laevigata three and 12 days after their application, respectively. Products formulated with chlorpyrifos and one with plant extracts were more efficient than granulated bait.

  8. First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae Primeiro registro de parasitismo social facultativo interespecífico em vespas do gênero Mischocyttarus Saussure (Hymenoptera, Vespidae

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    Thiago S. Montagna

    2012-06-01

    Full Text Available First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae. Parasitism of colonies of the social wasp Mischocyttarus cerberus Ducke, 1918 by females of Mischocyttarus consimilis Zikán, 1949 was observed in a rural area of Dourados, state of Mato Grosso do Sul, Brazil. In all monitored cases, the invasion occurred in the pre-emergence colony stage, generally by a single female of M. consimilis. The period of establishment of the foreign female in the host colony was marked by antagonistic behaviors between the host female and the invasive. In general, the architecture of the parasitized nest was modified from the typical architecture of the host species nest.Primeiro registro de parasitismo social facultativo interespecífico em vespas do gênero Mischocyttarus Saussure (Hymenoptera, Vespidae. Parasitismo de colônias da vespa social Mischocyttarus cerberus Ducke, 1918 por fêmeas de Mischocyttarus consimilis Zikán, 1949 foram registrados em uma área rural no município de Dourados estado de Mato Grosso do Sul no Brasil. Em todos os casos monitorados a invasão ocorreu na fase colonial de pré-emergência, e em geral foi executado por uma única fêmea de M. consimilis. O período de estabelecimento da fêmea estrangeira na colônia hospedeira foi marcado por comportamentos antagônicos entre as fêmeas interespecíficas. Em geral, a arquitetura do ninho parasitado foi modificada em relação à arquitetura típica do ninho da espécie hospedeira.

  9. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae in Brazil

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    A. L. Marsaro Júnior

    Full Text Available Abstract Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam. de Wit (Fabaceae, Morus nigra L. (Moraceae, Citrus reticulata Blanco (Rutaceae, Tectona grandis L. f. (Verbenaceae, Anacardium occidentale (Anacardiaceae, Annona squamosa L. and Xylopia aromatica (Lam. Mart. (Annonaceae, in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  10. Mitochondrial sequencing reveals five separate origins of 'black' Apis mellifera (Hymenoptera: Apidae) in eastern Australian commercial colonies.

    Science.gov (United States)

    Oxley, P R; Oldroyd, B P

    2009-04-01

    Establishment of a closed population honey bee, Apis mellifera L. (Hymenoptera: Apidae), breeding program based on 'black' strains has been proposed for eastern Australia. Long-term success of such a program requires a high level of genetic variance. To determine the likely extent of genetic variation available, 50 colonies from 11 different commercial apiaries were sequenced in the mitochondrial cytochrome oxidase I and II intergenic region. Five distinct and novel mitotypes were identified. No colonies were found with the A. mellifera mellifera mitotype, which is often associated with undesirable feral strains. One group of mitotypes was consistent with a caucasica origin, two with carnica, and two with ligustica. The results suggest that there is sufficient genetic diversity to support a breeding program provided all these five sources were pooled.

  11. Prediction of social structure and genetic relatedness in colonies of the facultative polygynous stingless bee Melipona bicolor (Hymenoptera, Apidae).

    Science.gov (United States)

    Dos Reis, Evelyze Pinheiro; de Oliveira Campos, Lucio Antonio; Tavares, Mara Garcia

    2011-04-01

    Stingless bee colonies typically consist of one single-mated mother queen and her worker offspring. The stingless bee Melipona bicolor (Hymenoptera: Apidae) shows facultative polygyny, which makes this species particularly suitable for testing theoretical expectations concerning social behavior. In this study, we investigated the social structure and genetic relatedness among workers from eight natural and six manipulated colonies of M. bicolor over a period of one year. The populations of M. bicolor contained monogynous and polygynous colonies. The estimated genetic relatedness among workers from monogynous and polygynous colonies was 0.75 ± 0.12 and 0.53 ± 0.16 (mean ± SEM), respectively. Although the parental genotypes had significant effects on genetic relatedness in monogynous and polygynous colonies, polygyny markedly decreased the relatedness among nestmate workers. Our findings also demonstrate that polygyny in M. bicolor may arise from the adoption of related or unrelated queens.

  12. Prediction of social structure and genetic relatedness in colonies of the facultative polygynous stingless bee Melipona bicolor (Hymenoptera, Apidae

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    Evelyze Pinheiro dos Reis

    2011-01-01

    Full Text Available Stingless bee colonies typically consist of one single-mated mother queen and her worker offspring. The stingless bee Melipona bicolor (Hymenoptera: Apidae shows facultative polygyny, which makes this species particularly suitable for testing theoretical expectations concerning social behavior. In this study, we investigated the social structure and genetic relatedness among workers from eight natural and six manipulated colonies of M. bicolor over a period of one year. The populations of M. bicolor contained monogynous and polygynous colonies. The estimated genetic relatedness among workers from monogynous and polygynous colonies was 0.75 ± 0.12 and 0.53 ± 0.16 (mean ± SEM, respectively. Although the parental genotypes had significant effects on genetic relatedness in monogynous and polygynous colonies, polygyny markedly decreased the relatedness among nestmate workers. Our findings also demonstrate that polygyny in M. bicolor may arise from the adoption of related or unrelated queens.

  13. A new species of Symbra (Hymenoptera, Eurytomidae, Heimbrinae from dry forest in Brazil and new occurrence records for other Heimbrinae

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    Daniell Rodrigo Rodrigues Fernandes

    2012-12-01

    Full Text Available The geographic ranges of Heimbra opaca (Ashmead, 1894, H. bicolor Subba Rao, 1978 and H. parallela Stage & Snelling, 1986 are extended based on study of material deposited in the entomological collections of the Laboratório de Sistemática e Bioecologia de Parasitoides e Predadores da APTA (Ribeirão Preto, SP, Brazil of the Instituto Brasileiro de Geografia e Estatística (Brasília, DF, Brazil. Symbra potiguara Perioto & Fernandes sp. nov. (Hymenoptera, Eurytomidae is described, illustrated and compared with S. cordobensis Stage & Snelling, 1986, the single species previously known for this genus. A key to the genera of Heimbrinae and to the species of Symbra is provided.

  14. A cuckoo in wolves' clothing? Chemical mimicry in a specialized cuckoo wasp of the European beewolf (Hymenoptera, Chrysididae and Crabronidae

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    Herzner Gudrun

    2008-01-01

    Full Text Available Abstract Background Host-parasite interactions are among the most important biotic relationships. Host species should evolve mechanisms to detect their enemies and employ appropriate counterstrategies. Parasites, in turn, should evolve mechanisms to evade detection and thus maximize their success. Females of the European beewolf (Philanthus triangulum, Hymenoptera, Crabronidae hunt exclusively honeybee workers as food for their progeny. The brood cells containing the paralyzed bees are severely threatened by a highly specialized cuckoo wasp (Hedychrum rutilans, Hymenoptera, Chrysididae. Female cuckoo wasps enter beewolf nests to oviposit on paralyzed bees that are temporarily couched in the nest burrow. The cuckoo wasp larva kills the beewolf larva and feeds on it and the bees. Here, we investigated whether H. rutilans evades detection by its host. Since chemical senses are most important in the dark nest, we hypothesized that the cuckoo wasp might employ chemical camouflage. Results Field observations suggest that cuckoo wasps are attacked by beewolves in front of their nest, most probably after being recognized visually. In contrast, beewolves seem not to detect signs of the presence of these parasitoids neither when these had visited the nest nor when directly encountered in the dark nest burrow. In a recognition bioassay in observation cages, beewolf females responded significantly less frequently to filter paper discs treated with a cuticular extract from H. rutilans females, than to filter paper discs treated with an extract from another cuckoo wasp species (Chrysis viridula. The behavior to paper discs treated with a cuticular extract from H. rutilans females did not differ significantly from the behavior towards filter paper discs treated with the solvent only. We hypothesized that cuckoo wasps either mimic the chemistry of their beewolf host or their host's prey. We tested this hypothesis using GC-MS analyses of the cuticles of male and

  15. First remarks on the nesting biology of Hypodynerus andeus (Packard (Hymenoptera, Vespidae, Eumeninae in the Azapa valley, northern Chile

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    Felipe Méndez-Abarca

    2012-06-01

    Full Text Available First remarks on the nesting biology of Hypodynerus andeus (Packard (Hymenoptera, Vespidae, Eumeninae in the Azapa valley, northern Chile. Some aspects about the nesting biology of the potter wasp Hypodynerus andeus (Packard, 1869 are reported for the first time. Observations were carried out at the Azapa valley, coastal desert of northern Chile. A total of sixty nests were collected and examined, each composed by 1-14 cells, most of them found attached to concrete lamp posts. The only preys recorded in the cells were Geometridae (Lepidoptera caterpillars and the presence of the parasitoid Anthrax sp. (Diptera, Bombyliidae was also recorded. A number of arthropods belonging to different groups, mainly spiders, were found occupying empty nests.

  16. Storage of Euschistus heros Eggs (Fabricius) (Hemiptera: Pentatomidae) in Liquid Nitrogen for Parasitization by Telenomus podisi Ashmead (Hymenoptera: Platygastridae).

    Science.gov (United States)

    Favetti, B M; Butnariu, A R; Doetzer, A K

    2014-06-01

    Records in the literature with regard to the influence of freezing of pentatomid eggs on parasitism by microhymenopterans are scarce. In this research, we compared the storage of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) eggs in liquid nitrogen for different periods with the objective of optimizing the multiplication of Telenomus podisi Ashmead (Hymenoptera: Platygastridae) in the laboratory. Fresh eggs of E. heros were exposed (S3, S6) or not (NS3, NS6) to UV light for 30 min and stored in 1.5-mL plastic vials in liquid nitrogen either for 3 (S3, NS3) or 6 months (S6, NS6), and egg suitability to parasitoid development was compared to control eggs exposed (SC) or not (NSC) to UV treatment. Global data analysis showed that E. heros eggs stored in liquid nitrogen with or without UV treatment, for 3 or 6 months, were suitable for T. podisi parasitization.

  17. Primary and secondary parasitoids (Hymenoptera) of aphids (Hemiptera: Aphididae) on blueberry and other Vaccinium in the Pacific Northwest.

    Science.gov (United States)

    Raworth, D A; Pike, K S; Tanigoshi, L K; Mathur, S; Graf, G

    2008-04-01

    Blueberry scorch virus, a commercially important Carlavirus in highbush blueberry, Vaccinium corymbosum L., is vectored by aphids (Hemiptera: Aphididae). We surveyed the aphids, primary parasitoids (Hymenoptera: Aphelinidae, Braconidae), and associated secondary parasitoids (Hymenoptera: Charipidae, Megaspilidae, Pteromalidae) on highbush blueberry and other Vaccinium in the Pacific Northwest from 1995 to 2006, with samples concentrated in 2005 and 2006, to lay the groundwork for augmentative biological control. Ericaphis fimbriata (Richards) was the principal aphid. The dominant parasitoid species were Praon unicum Smith, Aphidius n. sp., A. sp., and Aphidius ervi Haliday. Their frequency in relation to the other primary parasitoids varied significantly with geographical area; P. unicum dominated the frequency distribution in southwestern British Columbia, A. n. sp., west of the Cascades, and A. sp. and A. ervi east of the Cascades. Among the secondary parasitoids, pteromalids dominated, and their frequency in relation to the other secondary parasitoids was lowest in southwestern British Columbia. The parasitization rate for P. unicum and A. n. sp. in southwestern British Columbia increased from May or June to a maximum of 0.080 +/- 0.024 and 0.090 +/- 0.084 (SD), respectively, in late July or early August. P. unicum emerged in the spring 4 wk before A. n. sp. The parasitization rate for P. unicum was lower in conventional than organic fields. Whereas aphid density increased monotonically, P. unicum had two spring peaks. A simulation model showed that these peaks could reflect discrete generations. Releases of insectary-reared P. unicum at 150 or 450 DD above 5.6 degrees C, summing from 1 January, may effectively augment the natural spring populations by creating overlapping generations.

  18. Morphological, chemical and developmental aspects of the Dufour gland in some eusocial bees (Hymenoptera, Apidae: a review

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    Fábio Camargo Abdalla

    2006-06-01

    Full Text Available Morphological, chemical and developmental aspects of the Dufour gland in some eusocial bees (Hymenoptera, Apidae: a review. The present revision focused on the more recent data about the Dufour gland in some eusocial bees, taking in account general aspects of its morphology, secretion chemical nature, bio-synthetic pathway and development. Many functions have been attributed to this gland in eusocial bees, but none are convincing. With the new data about this gland, not only the secretion chemical pathway of the Dufour gland may be reasonable understood, as its function in some eusocial bees, especially Apis mellifera Linné, 1758, which has been extensively studied in the last years.Aspectos morfológicos, químicos e do desenvolvimento da glândula de Dufour em algumas abelhas eussociais (Hymenoptera, Apidae: revisão. Esta revisão aborda os mais recentes dados sobre a glândula de Dufour em algumas abelhas eussociais, considerando aspectos gerais da sua morfologia, do seu desenvolvimento, da natureza química da sua secreção, assim como sua via bio-sintética. Muitas funções têm-se atribuído à glândula de Dufour nas abelhas eussociais, mas nenhuma suficientemente convincente. Os novos dados a respeito dessa glândula permitem não só conhecer razoavelmente bem a via bio-sintética como a função da secreção da glândula de Dufour em algumas abelhas eussociais, especialmente em Apis mellifera Linné, 1758, a qual tem sido extensivamente estudada nos últimos anos.

  19. Meliponini neotropicais: o gênero Partamona Schwarz, 1939 (Hymenoptera, Apidae

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    Pedro Silvia R. M.

    2003-01-01

    Full Text Available Neotropical Meliponini: the genus Partamona Schwarz, 1939 (Hymenoptera, Apidae. The systematics and biogeography of Partamona Schwarz, a Neotropical genus of stingless bees (Meliponini, Apinae, Apidae, are revised. Seventeen new species are described: P. epiphytophila sp. nov., P. subtilis sp. nov., P. nhambiquara sp. nov., P. batesi sp. nov., P. yungarum sp. nov., P. vitae sp. nov., P. ferreirai sp. nov., P. gregaria sp. nov., P. auripennis sp. nov., P. nigrilabris sp. nov., P. combinata sp. nov., P. chapadicola sp. nov., P. seridoensis sp. nov., P. littoralis sp. nov., P. criptica sp. nov., P. rustica sp. nov. and P. sooretamae sp. nov. Partamona pseudomusarum Camargo, 1980, is considered as junior synonym of P. vicina Camargo, 1980. Types of P. grandipennis (Schwarz, 1951, P. xanthogastra Pedro & Camargo, 1996-1997, P. pearsoni (Schwarz, 1938, P. ailyae Camargo, 1980, P. pseudomusarum, P. vicina, P. mulata Moure in Camargo, 1980, P. aequatoriana Camargo, 1980, P. mourei Camargo, 1980, P. peckolti, (Friese, 1901, P. testacea (Klug, 1807, P. helleri (Friese, 1900 and P. musarum (Cockerell, 1917 were examined. Lectotypes of P. orizabaensis (Strand, 1919, and P. cupira (Smith, 1863 are designated. An identification key for the species and drawings of morphological characters are presented. A phylogenetic hypothesis, based mainly on morphological characters is proposed. Four groups are defined, considering the shape of mandible of workers and sternum VII of males: bilineata / epiphytophila group (western Amazon to México, including P. bilineata (Say, P. grandipennis, P. xanthogastra P. orizabaensis P. peckolti P. epiphytophila sp. nov., P. subtilis sp. nov., P. nhambiquara sp. nov., P. batesi sp. nov., P. yungarum sp. nov. and P. vitae sp. nov.; musarum group (Central Brazil, north of South America to Central America, including P. musarum, P. aequatoriana, P. vicina, P. mourei, P. pearsoni, P. ferreirai sp. nov., P. gregaria sp. nov. and P

  20. Hymenoptera parasitoides associados às larvas de Lepidoptera em reflorestamento e sistemas agroflorestais da fazenda Canchim (Embrapa Pecuária Sudeste), São Carlos, São Paulo, Brasil

    OpenAIRE

    Aline Garcia Pereira

    2013-01-01

    Estudos da flora e fauna silvestres são importantes por contribuirem para a compreensão dos processos ecológicos que ocorrem em resposta às estratégias de manejo utilizadas. Os Hymenoptera são um grupo-chave para o estabelecimento de prioridades em conservação do ambiente, pois representam alta proporção da diversidade de insetos, sendo facilmente amostrados. O objetivo deste trabalho foi caracterizar a fauna de Hymenoptera parasitoides associada às larvas de Lepidoptera em área de refloresta...