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Sample records for clematis jackmanii

  1. Kaempferol 3,7,4'-glycosides from the flowers of Clematis cultivars.

    Science.gov (United States)

    Sakaguchi, Keisuke; Kitajima, Junichi; Iwashina, Tsukasa

    2013-08-01

    A new kaempferol glycoside, kaempferol 3-O-alpha-rhamnopyranosyl-(1 --> 6)-beta-glucopyranoside-7,4'-di-O-beta-glucopyranoside (1) was isolated from the flowers of Clematis cultivars "Jackmanii Superba" and "Fujimusume", together with the known compound kaempferol 3,7,4'-tri-O-beta-glucopyranoside (2). The chemical structures of the isolated kaemferol glycosides were established by UV, 1H and 13C NMR spectroscopy, LC-MS, and characterization of acid hydrolysates. PMID:24079175

  2. Kaempferol tri- and tetraglycosides from the flowers of Clematis cultivar.

    Science.gov (United States)

    Sakaguchi, Keisuke; Kitajima, Junichi; Iwashina, Tsukasa

    2012-02-01

    A new kaempferol glycoside, kaempferol 3-O-alpha-rhamnopyranosyl-(1 --> 2)-[alpha-rhamnopyranosyl-(1 --> 6)-beta-glucopyranoside]-7-O-beta-glucopyranoside (2) was isolated from the flowers of Clematis cultivar "Jackmanii Superba", together with a known kaempferol 3-O-alpha-rhamnopyranosyl-(1 --> 6)-beta-glucopyranoside-7-O-beta-glucopyranoside (1). The chemical structures of the isolated glycosides were established by UV, LC-MS, characterization of acid hydrolysates, and 1H and 13C NMR spectroscopy. PMID:22474946

  3. Acylated delphinidin glycosides from violet and violet-blue flowers of Clematis cultivars and their coloration.

    Science.gov (United States)

    Sakaguchi, Keisuke; Kitajima, Junichi; Iwashina, Tsukasa

    2013-11-01

    Three new acylated delphinidin glycosides, delphinidin 3-O-beta-[(2"-trans-caffeoylglucopyranosyl)-(1 --> 2)-(6"-succinylgalactopyranoside)]-7-O-beta-glucopyranoside (1), delphinidin 3-O-beta-[(2"-trans-caffeoylglucopyranosyl)-(1 --> 2)-(6"-trans-caffeoyl-tartaroyl-malonylgalactopyranoside)]-7-O-beta-glucopyranoside (2), and delphinidin 3-O-beta-[(2"-trans-caffeoylglucopyranosyl)-(1 --> 2)-(6"-trans-caffeoyl-tartaroyl-malonylgalactopyranoside)]-3'-O-beta-glucuronopyranoside (3), were isolated from the violet and violet-blue sepals of Clematis cultivars 'Jackmanii Superba' and 'Fujimusume'. The chemical structures of the isolated anthocyanins were determined by LC-MS, characterization of hydrolyzates, and UV, 1H and 13C NMR spectroscopy. The visible absorption spectra of these anthocyanins were compared with those of fresh sepals and crude extracts in pH 5.1 buffer solution. In addition, the co-pigment effect with some kaempferol glycosides and caffeoylglucose was examined. PMID:24427942

  4. Meclatis in Clematis: yellow flowering Clematis species : systematic studies in Clematis L. (Ranunculaceae), inclusive of cultonomic aspects

    OpenAIRE

    Brandenburg, W.A.

    2000-01-01

    The general classification of the genus Clematis (Ranunculaceae) was subject of study in chapter 1. Based on species character scores, the infrageneric classification was analyzed by applying Hennig86 as phylogenetical analysis package. As result of this analysis Clematis was subdivided into 18 sections, one of them subdivided in 3 subsections.The world distribution of Clematis was also studied with Hennig86. It was not possible to postulate the area of origin of the genus Clematis with the a...

  5. Meclatis in Clematis: yellow flowering Clematis species : systematic studies in Clematis L. (Ranunculaceae), inclusive of cultonomic aspects

    NARCIS (Netherlands)

    Brandenburg, W.A.

    2000-01-01

    The general classification of the genus Clematis (Ranunculaceae) was subject of study in chapter 1. Based on species character scores, the infrageneric classification was analyzed by applying Hennig86 as phylogenetical analysis package. As result of this analysis Clematis was subdivided into 18 sect

  6. Confirmation of Clematis hybrids using molecular markers

    Science.gov (United States)

    The hybrid origin of two progeny from reciprocal crosses of Clematis tubulosa and C. brevicaudata was investigated using molecular markers generated by randomly amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and single nucleotide polymorphisms (SNPs). Morphologi...

  7. A revision of Clematis sect. Atragene (Ranunculaceae)

    Institute of Scientific and Technical Information of China (English)

    Wen-Jing YANG; Liang-Qian LI; Lei XIE

    2009-01-01

    Clematis sect. Atragene is revised in this paper based on the examination of a large number of herbarium specimens, extensive field observations, and morphometric analyses. Brief taxonomic history and geographical distribution of the section are given, the relationships among the species are discussed, and the evolutionary trends of some characters in the section are evaluated. The staminodes of the plants in this section may have evolved from the outer stamens with petaloid filaments and gradually disappearing anthers. Subsequently, they may have evolved in two different ways. One possibility is that the staminodes elongate and become lanceolate, as long as sepals, and their apices turn into attenuate. The other is that the staminodes are spathulate, but not elongating, as long as stamens, and their apices turn into retuse from obtuse and rounded. The evolutionary trend of sepals may be from thin to thick in texture, and the veins from non-prominent to prominent. As a result, five new series are established and nine species, two subspecies and nine varieties (including three new ranks) are recognized in this section. An identification key is provided, and each taxon is described and illustrated. Clematis sibirica and Clematis ochotensis are treated as subspecies of Clematis alpina due to their subtle differences and lack of, or few, overlapping distributions. Clematis fusijamana and Clematisfauriei are recognized as varieties of C. alpina ssp. ochotensis for the continuous variation of the velutinous strips on the sepal margins. Clematis iliensis is treated as variety of C. alpina ssp. sibirica for the continuous variation of leaf division types. Extensive variations in sepal color and basal caruncle size support degrading Clematis chiisanensis as a variety of Clematis koreana. The North American ser. Occientales may be primitive, whereas ser. Macropetalae may be the most advanced taxon in this section. Ser. Alpinae and ser. Koreanae are closely related to each

  8. [Investigation of molecular-genetic heterogeneity of clematis plants (Clematis L.) obtained by organogenesis and somatic embryogenesis in vitro].

    Science.gov (United States)

    Mitrofanova, I V; Galaev, A V; Sivolap, Iu M

    2003-01-01

    Genome variability of in vitro micropropagated Clematis plants was established. The optimum concentrations of BAP and zeatin in the culture medium regulating in vitro morphogenetic processes in clematis explants cv. Serenada Kryma were determined. Molecular-genetic analysis of Clematis plants obtained via in vitro somatic embryogenesis and organogenesis was carried out. Using ISSR primers 105 amplicons have been revealed, six of them were polymorphic. The mean index of heterogeneity of clematis plants was 5.7%. PMID:15067940

  9. Karyotype features of 10 species of Clematis and cluster analysis of karyotypies resemblance-near coefficient%10种铁线莲的核型特征及核型似近系数聚类分析

    Institute of Scientific and Technical Information of China (English)

    彭绿春; 于恒隽; 余娜; 瞿素萍; 张艺萍; 解玮佳; 李世峰; 丁鲲

    2012-01-01

    Taking young root tip of in vitro plantlet of 10 species of Clematis as material, chromosome feature analysis and cluster analysis of karyotype resemblance-near coefficient were conducted by using conventional pressed slice method, to discuss the karyotype features and genetic relationship between species. The results suggested that the chromosome number of 'Jackmanii'was 14, the chromosome number of the other species were 16, and basic number of chromosome was 8. There existed differences of karyotype parameters in 10 species of Clematis, so karyotype datas could provide a base for the classification and genetic relationship identification of Clematis. To make cluster analysis of karyotypies resemblance-near coefficient, when karyotype resemblance-near coefficient(λ) was 0.818 1, 10 species of Clematis could be divided into 3 groups, when λ was 0.893 9, 10 species of Clematis could be divided into 4 groups.%为探明铁线莲核型特征与种间的亲缘关系,以10种(品种)铁线莲组培苗幼嫩根尖为材料,应用常规压片法分析染色体特征,并进行核型似近系数聚类分析.核型特征分析结果表明:除1个栽培种‘杰克曼’的染色体数为14外,其余9个种的染色体数2n=16,染色体基数为8;10种铁线莲核型特征参数种间存在的差异,可作为铁线莲属植物分类和亲缘关系判定的依据.以核型似近系数进行聚类分析,在核型似近系数为0.818 1时,10种铁线莲聚为3类;在核型似近系数为0.893 9时,10种铁线莲聚为4类.

  10. Chemical Constituents from Clematis delavayi var. spinescens

    Directory of Open Access Journals (Sweden)

    Xiao-Li Li

    2009-11-01

    Full Text Available A new coumarin, 7-hydroxy-4,6-dimethoxy-5-methylcoumarin (1, was isolated from the aerial parts of Clematis delavayi var. spinescens together with 17 known compounds. Their structures were identified by extensive spectral analysis, especially 2D NMR techniques. Antiangiogenic effects of all compounds were evaluated using a zebrafish model.

  11. Three new triterpene saponins from Clematis chinensis.

    Science.gov (United States)

    Fu, Qiang; Zan, Ke; Zhao, Ming-Bo; Zhou, Si-Xiang; Shi, She-Po; Jiang, Yong; Tu, Peng-Fei

    2013-01-01

    Three new triterpene saponins, clematochinenosides H-J (1-3), were isolated from the roots and rhizomes of Clematis chinensis. Their structures were elucidated on the basis of spectroscopic means including 1D and 2D NMR experiments and hydrolysis products. PMID:23659476

  12. A new triterpenoid saponin from Clematis ganpiniana

    Institute of Scientific and Technical Information of China (English)

    Feng Sun; Qing He; Pei Gen Xiao; Yi Yu Cheng

    2007-01-01

    A new triterpenoid saponin, named clematiganoside A (1), was isolated from the whole plant of Clematis ganpiniana. Its structure was elucidated on the basis of 1D, 2D NMR, TOF-MS and ESI-MS techniques, and physicochemical properties.

  13. Chemical Constituents from Clematis delavayi var. spinescens

    OpenAIRE

    Xiao-Li Li; Yong-Ping Yang; Xi-Min Wang; Ke-Chun Liu; Si-Feng Wang; Yan-Li Zhao; Yang Li

    2009-01-01

    A new coumarin, 7-hydroxy-4,6-dimethoxy-5-methylcoumarin (1), was isolated from the aerial parts of Clematis delavayi var. spinescens together with 17 known compounds. Their structures were identified by extensive spectral analysis, especially 2D NMR techniques. Antiangiogenic effects of all compounds were evaluated using a zebrafish model.

  14. Protoanemonin content variation between Clematis spp.: leaf, stem and root.

    Science.gov (United States)

    Jin, Fangming; Narkowicz, Christian; Jacobson, Glenn A

    2013-02-01

    The content of protoanemonin, a known biologically active constituent of Clematis spp., was determined by GC-MS in the leaf, stem and root extracts of one Chinese species and four Australian Clematis taxa. The results showed that protoanemonin concentrations varied between different plants and that leaves contained higher concentrations than stems and roots. To our knowledge this is the first study to determine protoanemonin content variation in leaf, stem and root of Clematis spp. PMID:23513731

  15. The reproductive biology of Clematis addisonii

    OpenAIRE

    Edwards, Rhonda L.

    1995-01-01

    Clematis addisonii Britton (Ranunculaceae) is a Virginia endemic restricted to calcareous soils in a four county region of the Ridge and Valley Province in Virginia. A two year study of the reproductive biology of this species reveals that it is self-compatible, showing no significant reduction in fecundity following self-pollinations. Morphological observations indicate that this species is protogynous. In vivo pollen tube growth supports this conclusion. Field observ...

  16. Lectotypification and description of Clematis viticella L. (Ranunculaceae)

    NARCIS (Netherlands)

    Brandenburg, W.A.; Vooren, van de J.G.; Jarvis, C.E.

    2001-01-01

    The typification of Clematis viticella L. is discussed, and a lectotype designated from the Clifford Herbarium (BM). A standard specimen for C. viticella‘Purpurea Plena Elegans’ has also been selected. Data from hybridization experiments and studies of natural distribution suggest that Clematis camp

  17. Clematis Chlorotic Mottle Virus, a novel virus occurring in Clematis in the USA

    Science.gov (United States)

    Clematis is a genus of temperate climbing vines that are popular as ornamentals. Samples from domestic and international sources showing symptoms of yellow mottling and veining, chlorotic ring spots, line pattern mosaics, and in some cases flower distortion and discoloration were received at several...

  18. Cytotoxic triterpene saponins from Clematis mandshurica.

    Science.gov (United States)

    He, Yu-Xin; Li, Ling; Zhang, Kang; Liu, Zhong-Rong

    2011-12-01

    Two new triterpene saponins, mandshunosides A and B (1 and 2), were isolated from the roots and rhizomes of Clematis mandshurica. Their structures were elucidated on the basis of spectroscopic evidence and hydrolysis products. Compounds 1 and 2 showed inhibitory activities against two colorectal human cancer cells HCT 116 (IC₅₀ 2.1 μM for 1 and 2.5 μM for 2) and HT-29 (IC₅₀ 3.7 μM for 1 and 3.3 μM for 2). PMID:22115034

  19. Analysis of the Lightcurve of 1101 Clematis

    Science.gov (United States)

    Warner, Brian D.; Stephens, Robert D.

    2010-04-01

    We report on our collaboration to obtain photometric data on the outer main-belt asteroid, 1101 Clematis. Data obtained in 2009 September yield a synodic rotation period of 34.3 ± 0.1 h and lightcurve amplitude of 0.16 ± 0.02 mag. The period spectrum shows a possible period at ~18.4 h but the phased lightcurve plot shows this solution is unlikely. The period of 34.3 h differs significantly from previously reported results.

  20. [In vitro propagation of Clematis filamentosa].

    Science.gov (United States)

    Shao, Ling; Yu, Ganxin

    2005-05-01

    The rapid propagation of Clematis filamentosa Dumn by tissue cluture showed that the best explant was young stem. It also showed that the callus can be easily induced in MS + NAA 0.1 mg/L + 6-BA 0.5 mg/L, the medium for the buds differentiation and proliferation was MS + NAA 0.05 mg/L + 6-BA 0.5 mg/L, and the medium for the root growth was MS + NAA 0.1 mg/L. The survival tube seedling can be successfully transplant. PMID:16131027

  1. Occurrence of blockage in cut stems of Clematis L.

    OpenAIRE

    Agata Jędrzejuk; Julia Rochala

    2013-01-01

    During vase life of cut flowers obstructions in stem xylem vessels develop. Such obstructions may restrict water uptake in stems and its transport towards flowers, thus lowering their ornamental value and longevity. Clematis is a very attractive plant which can be used as a cut flower in floral compositions. However, nothing is known about the histochemical or cytolo- gical nature of xylem blockages occurring in cut stems of this plant. Observations carried out on Clematis cv. 'Solidarność' p...

  2. Note: Aphis vitalbae Ferrari, 1872 (Hemiptera: Aphididae): new finding on ornamental Clematis spp. in Poland

    OpenAIRE

    Hałaj, Roman; Osiadacz, Barbara

    2015-01-01

    Aphis vitalbae Ferrari is reported from Poland for the first time on ornamental clematis (Clematis spp.). The distribution of this aphid in Europe and its possible expansion route are presented with a key to identifying the aphids infesting Clematis plants in Europe. Barbara Osiadacz

  3. The complete chloroplast genome sequence of Clematis terniflora DC. (Ranunculaceae).

    Science.gov (United States)

    Li, Mengzhu; Yang, Bingxian; Chen, Qinyi; Zhu, Wei; Ma, Ji; Tian, Jingkui

    2016-07-01

    Clematis terniflora DC. is an important medicinal plant used in the treatment of inflammatory symptoms related to respiratory and urinary systems. In this study, we found that the complete cp genome of C. terniflora DC. is 159,528 bp. The phylogenetic analysis of 32 taxa showed a strong sister relationship with Ranunculus macranthus, which also strongly supports the position of Ranunculales. The complete cp genome sequence of Clematis terniflora DC. reported here has the potential to advance population and phylogenetic studies of this medicinal plant. PMID:25865739

  4. Triterpenoid saponins from the roots of Clematis argentilucida.

    Science.gov (United States)

    Zhao, Mei; Ma, Ning; Qiu, Feng; Tian, Xiangrong; Zhang, Yan; Tang, Haifeng; Liu, Xinyou

    2014-09-01

    Reinvestigation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of a new ursane-type triterpenoid saponin 1 and a new taraxerane-type saponin 2, four known saponins 3-6 first isolated from the species, together with seven saponins 7-13 reported in the previous papers. The structures of saponins 1-6 were elucidated by extensive spectroscopic analysis and chemical evidences. The ursane-type and taraxerane-type triterpenoid saponins were obtained from genus Clematis for the first time, and the aglycone of saponin 1, 3β,28-dihydroxy-18αH-ursan-20-en was first encountered. The cytotoxicity of all the saponins was evaluated against human glioblastoma U251MG cell lines. The monodesmosidic saponins 1, 2 and 4-8 exhibited cytotoxic activity against the cells with IC50 values ranging from 6.95 to 38.51 μM. PMID:24979221

  5. ANTIMICROBIAL ACTIVITY OF CLEMATIS BRACHIATA THUNB LEAF EXTRACTS

    OpenAIRE

    M. Mostafa* and A.J. Afolayan

    2013-01-01

    The antimicrobial activity as well as phytochemical screening of the hexane, acetone, methanol and water extracts of Clematis brachiata Thunb (Ranunculaceae) leaves was investigated. The agar dilution assay method was used for the evaluation of the antimicrobial activity of extracts against 10 bacteria and four fungal species. The phytochemical screenings were performed by using the standard procedures. The acetone and methanol extracts were active against the 10 bacteria strains with MIC ran...

  6. Triterpene saponins from Clematis mandshurica and their antiproliferative activity.

    Science.gov (United States)

    Gong, Yi-Xia; Hua, Hui-Ming; Xu, Yong-Nan; Liu, Jian-Yu; Yu, Zong-Gui; Ma, Jing; Zhang, Hui; Jing, Yong-Kui

    2013-07-01

    Six new triterpene saponins, clematomandshurica saponins F-K (1-6), together with a known compound (7), were isolated from the roots and rhizomes of Clematis mandshurica. Their structures were elucidated on the basis of spectroscopic evidence and hydrolysis. Compounds 5-7 exhibited antiproliferative effects against PC-3 human prostate cancer cells with GI50 values of 1.29, 1.50, and 0.71 µM, respectively. PMID:23804038

  7. Two Novel Antifungal Saponins from Tibetan Herbal Medicine Clematis tangutica

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Antifungal assay-guided isolation of the ethanol extract of the aerial parts of Clematis tangutica yielded two novel triterpene saponins. Their structures were determined to be 3-O-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl ester (1) and 3-O-β-D- glucopyranosyl-(1→4)-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl ester (2) on the basis of spectral data and chemical reactions.

  8. Triterpenoid saponins from the roots of Clematis uncinata.

    Science.gov (United States)

    Li, Shuo-Guo; Huang, Xiao-Jun; Li, Man-Mei; Wang, Mei; Feng, Rui-Bing; Zhang, Wei; Li, Yao-Lan; Wang, Ying; Ye, Wen-Cai

    2014-01-01

    Eight new bisdesmosidic triterpenoid saponins, clematiunicinosides A-H (1-8), along with eleven known ones (9-19), were isolated from the roots of Clematis uncinata. Their structures were elucidated on the basis of spectroscopic analysis and chemical evidence. All the isolated saponins were tested for their cytotoxic activities on human caski cervical cancer (Caski) cells, and compounds 13, 17 and 19 exhibited inhibitory effect on Caski cells. PMID:24152568

  9. Clematis austroanatolica (Ranunculaceae), an unusual new species from southern Anatolia, Turkey

    DEFF Research Database (Denmark)

    Zielinski, Jerzy; Tan, Kit

    2011-01-01

    Clematis austroanatolica (Ranunculaceae) is illustrated and described as a new species endemic to southern Anatolia, Turkey. It is related to C. vitalba and differs from the latter in having leaves with narrowly ovate, dentate-serrate leaflets, trifoliolate lowermost pinnae as well as small, pale...... pink flowers with orange-brown filaments. A key to the Turkish taxa of Clematis is provided....

  10. Occurrence of blockage in cut stems of Clematis L.

    Directory of Open Access Journals (Sweden)

    Agata Jędrzejuk

    2013-04-01

    Full Text Available During vase life of cut flowers obstructions in stem xylem vessels develop. Such obstructions may restrict water uptake in stems and its transport towards flowers, thus lowering their ornamental value and longevity. Clematis is a very attractive plant which can be used as a cut flower in floral compositions. However, nothing is known about the histochemical or cytolo- gical nature of xylem blockages occurring in cut stems of this plant. Observations carried out on Clematis cv. 'Solidarność' proved that tyloses appeared as a principal source of xylem blockage in cut stems. The preservative composed of 200 mg × dm-3 8-HQC (8-hydroxyquinolin citrate and 2% sucrose arre-sted development of xylem blockage, while the vessels in stems kept in water were filled with tyloses or an amorphic substance. PAS reaction proved that polysaccharides were present in the xylem occlusions, whereas no homogalacturonans were immunolocalized in tyloses using JIM 5 and JIM 7 antibodies. The present study provides new information on the origin of xylem occlusions in clematis and their development in two different vase solutions. Such information can be useful to develop pro- per postharvest treatments aiming to improve keeping qualities of this new cut flower.

  11. Identification of Xylem Occlusions Occurring in Cut Clematis (Clematis L., fam. Ranunculaceae Juss.) Stems during Their Vase Life

    OpenAIRE

    Agata Jedrzejuk; Julia Rochala; Jacek Zakrzewski; Julita Rabiza-Świder

    2012-01-01

    During the vase life of cut stems obstruction of xylem vessels occurs due to microbial growth, formation of tyloses, deposition of materials in the lumen of xylem vessels and the presence of air emboli in the vascular system. Such obstructions may restrict water uptake and its transport towards upwards thus lowering their ornamental value and longevity of cut flowers. Clematis is a very attractive plant material which may be used as cut flower in floral compositions. Nothing is known about th...

  12. Identification of Xylem Occlusions Occurring in Cut Clematis (Clematis L., fam. Ranunculaceae Juss. Stems during Their Vase Life

    Directory of Open Access Journals (Sweden)

    Agata Jedrzejuk

    2012-01-01

    Full Text Available During the vase life of cut stems obstruction of xylem vessels occurs due to microbial growth, formation of tyloses, deposition of materials in the lumen of xylem vessels and the presence of air emboli in the vascular system. Such obstructions may restrict water uptake and its transport towards upwards thus lowering their ornamental value and longevity of cut flowers. Clematis is a very attractive plant material which may be used as cut flower in floral compositions. Nothing is known about the histochemical or cytological nature of xylem blockages occurring in cut stems of this plant. This study shows that in clematis, tyloses are the main source of occlusions, although bacteria and some amorphic substances may also appear inside the vessels. A preservative composed of 200 mg dm−3 8-HQC (8-hydroxyquinolin citrate and 2% sucrose arrested bacterial development and the growth of tyloses. This information can be helpful in the development of new treatments to improve keeping qualities of cut clematis stems.

  13. Identification of xylem occlusions occurring in cut clematis (Clematis L., fam. Ranunculaceae Juss.) stems during their vase life.

    Science.gov (United States)

    Jedrzejuk, Agata; Rochala, Julia; Zakrzewski, Jacek; Rabiza-Świder, Julita

    2012-01-01

    During the vase life of cut stems obstruction of xylem vessels occurs due to microbial growth, formation of tyloses, deposition of materials in the lumen of xylem vessels and the presence of air emboli in the vascular system. Such obstructions may restrict water uptake and its transport towards upwards thus lowering their ornamental value and longevity of cut flowers. Clematis is a very attractive plant material which may be used as cut flower in floral compositions. Nothing is known about the histochemical or cytological nature of xylem blockages occurring in cut stems of this plant. This study shows that in clematis, tyloses are the main source of occlusions, although bacteria and some amorphic substances may also appear inside the vessels. A preservative composed of 200 mg dm(-3) 8-HQC (8-hydroxyquinolin citrate) and 2% sucrose arrested bacterial development and the growth of tyloses. This information can be helpful in the development of new treatments to improve keeping qualities of cut clematis stems. PMID:22919351

  14. Identification of Xylem Occlusions Occurring in Cut Clematis (Clematis L., fam. Ranunculaceae Juss.) Stems during Their Vase Life

    Science.gov (United States)

    Jedrzejuk, Agata; Rochala, Julia; Zakrzewski, Jacek; Rabiza-Świder, Julita

    2012-01-01

    During the vase life of cut stems obstruction of xylem vessels occurs due to microbial growth, formation of tyloses, deposition of materials in the lumen of xylem vessels and the presence of air emboli in the vascular system. Such obstructions may restrict water uptake and its transport towards upwards thus lowering their ornamental value and longevity of cut flowers. Clematis is a very attractive plant material which may be used as cut flower in floral compositions. Nothing is known about the histochemical or cytological nature of xylem blockages occurring in cut stems of this plant. This study shows that in clematis, tyloses are the main source of occlusions, although bacteria and some amorphic substances may also appear inside the vessels. A preservative composed of 200 mg dm−3 8-HQC (8-hydroxyquinolin citrate) and 2% sucrose arrested bacterial development and the growth of tyloses. This information can be helpful in the development of new treatments to improve keeping qualities of cut clematis stems. PMID:22919351

  15. Triterpenoid saponins from Clematis tangutica and their cardioprotective activities.

    Science.gov (United States)

    Zhang, Wei; Wang, Xiaoyang; Tang, Haifeng; Wang, Minchang; Ji, Lanju; Wen, Aidong; Wang, Jianbo

    2013-01-01

    Phytochemical investigation of the whole plants of Clematis tangutica led to the isolation of three new triterpenoid saponins (1-3), together with four known saponins (4-7). Their structures were determined by extensive spectral analysis and chemical evidences. Compounds 1-7 were evaluated for their cardioprotective activities in cardiomyocytes anoxia/reoxygenation (A/R) model. The results showed that those saponins exhibited cardioprotective effects by decreasing the levels of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH). PMID:23266737

  16. A New Flavone C-Glycoside from Clematis rehderiana

    OpenAIRE

    Zhi-Zhi Du; Xian-Wen Yang; Hao Han; Xiang-Hai Cai; Xiao-Dong Luo

    2010-01-01

    A new flavone C-glycoside, isovitexin 6″-O-E-p-coumarate (1) and two known flavonoid glycosides—quercetin 3-O-β-D-glucuronopyranoside (2) and isoorientin (3)—were isolated from an ethanol extract of aerial parts of Clematis rehderiana. Their structures were determined by spectroscopic methods. The antioxidant effects of the two flavone C-glycosides were evaluated by both the MTT and DPPH assays. Compound 1 showed potent activities against H2O2-induced impairment in PC12 cells within the conce...

  17. The hypotensive action of Desmodium styracifolium and Clematis chinensis.

    Science.gov (United States)

    Ho, C S; Wong, Y H; Chiu, K W

    1989-01-01

    The cardiovascular pharmacology of aqueous extracts of Desmodium styracifolium (DSE) and Clematis chinensis (CCE) were studied in rats both in vivo and in vitro. DSE produced two successive hypotensive actions: the first one via cholinergic receptor stimulation, while the second one potentiated by blockades of autonomic ganglion and alpha-adrenoceptor. In contrast to DSE, CCE produced only one hypotensive response which was mediated through histaminergic activity. Furthermore, both extracts relaxed isolated methoxamine preconstricted helical tail artery strips. CCE also produced both negative chronotropic and inotropic effects on isolated atria, while DSE was positive chronotropic without apparent effect on the contractile force. PMID:2633620

  18. 铁线莲属威灵仙组修订(续)%A revision of Clematis sect. Clematis (Ranunculaceae) (Continued)

    Institute of Scientific and Technical Information of China (English)

    王文采

    2003-01-01

    @@ Subsection 4. Angustifoliae Tamura in Sci. Rep. Osaka Univ. 4: 55. 1955 et 16 (2): 33. 1967; M. C. Chang in Fl. Reip. Pop. Sin. 28: 156. 1980. --Sect. Flammula DC. Subsect. Angustifoliae (Tamura) Tamura in Acta Phytotax. Geobot. 16: 81. 1956 et 38: 43. 1987; M. Johnson, Klematis 603. 1997; Grey_Wils., Clematis 113. 2000. -- Sect. Angustifoliae (Tamura) Serov in Bot. Zhurn. 73 (12): 1740. 1988; Tamura in Heipko, Engler's Nat. Pflanzenfam., ed. 2, 17a (4): 383. 1995. Type: C. Hexapetala Pall.

  19. Two new phenolic glycosides from the stems of Clematis parviloba.

    Science.gov (United States)

    Yan, Li-hua; Xu, Li-zhen; Wang, Zhi-min; Zhang, Qi-wei; Yang, Shi-lin

    2010-12-01

    To study the chemical constituents of the stems of Clematis parviloba, six compounds were isolated from a 95% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel, ODS, Sephadex LH-20, and semi-preparative HPLC. Two new phenolic glycosides, 2-((E)-3-carboxybut-2-en-yl)-4-hydroxy-3-methyl-phenyl-O-beta-D-glucopyranoside (1) and 4'-hydroxy-phenol-beta-D-[6-O-(4"-hydroxy-3", 5"-dimethoxy-benzoate)] glucopyranoside (2) were isolated, together with a known phenolic glycoside, 4'-hydroxy-3'-methoxy-phenol-beta-D-[6-O-(4"-hydroxy-3", 5"-dimethoxy-benzoate)] glucopyranoside (3) as well as three known megastigmane glycosides, linarionoside A (4), linarionoside C (5), and staphylionoside K (6). Their structures were determined on the basis of spectroscopic analysis and chemical evidence. Among them, compounds 1 and 2 were named as clemaparvilosides A (1) and B (2), respectively, and compounds 3-6 were obtained from Clematis genus for the first time. PMID:21348422

  20. 四川铁线莲属二新种%Two new species of Clematis (Ranunculaceae)from Sichuan

    Institute of Scientific and Technical Information of China (English)

    王文采; 李良千

    2012-01-01

    Two species of the genus Clematis (Ranunculaceae) ,C. huangjingensis, belonging to sect. Clematis subsect. Clematis and C. gulinensis,belonging to sect. Viorna (Reichb. ) Prantl subsect. Connatae Koehne,are described as new from Sichuan,China.%描述了自四川东南部发现的毛茛科铁线莲属二新种:黄荆铁线莲和古蔺铁线莲.

  1. Cytology and palynology of the Clematis L. species (Ranunculaceae) in Iran

    OpenAIRE

    Sheidai, Masoud; Habibi, Meysam; Azizian, Dina; Khatamsaz, Mahboobeh

    2009-01-01

    Cytological and palynological studies were performed on Clematis L. species (Runanculaceae) of Iran indicating 2n = 2´ = 16 and 2n = 4´ = 32 in them. They formed only bivalents in metaphase of meiosis-I with some amount of chromosome stickiness and laggard formation in anaphase. The species possessed a symmetrical karyotype but differed in karyotypic formulae indicating the occurrence of structural changes in the chromosomes during species diversification. Clematis species usually possessed t...

  2. Wound healing activity of the methanol extracts of Clematis species indigenous to Ethiopia

    OpenAIRE

    Segewkal Hawaze; Habtewold Deti; Sultan Suleman

    2013-01-01

    Objective: To investigate the vulnerary activity of the methanol extracts of Clematis longicauda steud ex A. Rich, and Clematis burgensis Engl leaves. Materials and Methods: In vivo wound healing activity of methanol extracts of C. longicauda and C. burgensis were studied using excision wound model, re-sutured incision wound model, histopathological analysis and anti-inflammatory activity test. The variables studied were percentage of wound contraction and epithelialisation period in excision...

  3. A new taraxastane-type triterpenoid glycoside from the roots of Clematis uncinata.

    Science.gov (United States)

    Li, Shuo-Guo; Li, Man-Mei; Zhao, Bing-Xin; Wang, Ying; Ye, Wen-Cai

    2015-01-01

    A new taraxastane-type triterpenoid glycoside, clematiunicinoside I (1), together with four known ones (2-5), was isolated from the roots of Clematis uncinata. The structure of the new compound was elucidated on the basis of spectroscopic analyses and acid hydrolysis. The cytotoxic activities of all the compounds against caski cervical cancer (Caski) cells were evaluated. This is the first report of the presence of taraxastane-type triterpenoid glycoside in the genus Clematis. PMID:26651184

  4. In vitro Antimicrobial Activity and Phytochemical Screening of Clematis Species Indigenous to Ethiopia

    OpenAIRE

    S Hawaze; Deti, H.; Suleman, S.

    2012-01-01

    The leaves extracts of two indigenous plants of Ethiopia: Clematis longicauda steud ex A. Rich. and Clematis burgensis Engl. are used in Southwestern Ethiopia to treat otorrhoea and eczema. Antimicrobial activity and MIC of crude extracts were determined by disk diffusion and broth dilution. Phytochemical screening was performed on the extracts. The methanol and petroleum ether extracts of both plants showed antibacterial and antifungal activity. Sensitivity of reference strains was concentra...

  5. Patrones asociados a la conducta de desplazamiento local en Phymactis clematis Drayton (Anthozoa: Actiniidae) Patterns of local movement behavior in Phymactis clematis Drayton (Anthozoa: Actiniidae)

    OpenAIRE

    Rivadeneira, Marcelo M.; EDUARDO OLIVA

    2001-01-01

    Phymactis clematis Drayton es la especie de actinia más abundante y unas de las que alcanza mayor tamaño en la costa de Sudamérica. Al igual que en otras especies de actinias, los adultos de la especie poseerían una activa capacidad de realizar desplazamientos locales. Sin embargo estos no han sido adecuadamente descritos, desconociéndose los factores causales. En este trabajo pretendemos describir algunos patrones básicos asociados a la conducta de desplazamiento local en P. clematis, evalua...

  6. Clematomandshurica saponin E, a new triterpenoid saponin from Clematis mandshurica.

    Science.gov (United States)

    Dong, Fang-Yan; Cui, Guo-He; Zhang, Ying-Hua; Zhu, Ruo-Nan; Wu, Xiu-Juan; Sun, Ting-Ting; Wang, Wei

    2010-12-01

    A new triterpenoid saponin, clematomandshurica saponin E, together with four known saponins were isolated and characterized from the roots and rhizomes of Clematis mandshurica (Ranunculaceae), a commonly used traditional Chinese medicine with anti-inflammatory and antirheumatoid activities. On the basis of spectroscopic analysis, including HR-ESI-MS, IR, 1D, and 2D NMR spectral data and hydrolysis followed by chromatographic analysis, the structure of the new triterpenoid saponin was elucidated as 3-O-α-L-rhamnopyranosyl-(1 → 6)-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 4)-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl oleanolic acid 28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside. PMID:21128147

  7. A New Flavone C-Glycoside from Clematis rehderiana

    Directory of Open Access Journals (Sweden)

    Zhi-Zhi Du

    2010-01-01

    Full Text Available A new flavone C-glycoside, isovitexin 6″-O-E-p-coumarate (1 and two known flavonoid glycosides—quercetin 3-O-β-D-glucuronopyranoside (2 and isoorientin (3—were isolated from an ethanol extract of aerial parts of Clematis rehderiana. Their structures were determined by spectroscopic methods. The antioxidant effects of the two flavone C-glycosides were evaluated by both the MTT and DPPH assays. Compound 1 showed potent activities against H2O2-induced impairment in PC12 cells within the concentration range tested, whereas compound 3 scavenged DPPH radical strongly, with an IC50 value of 13.5 μM.

  8. ANATOMIA CANTITATIVĂ A LAMINEI FRUNZEI LA UNELE SPECII DE CLEMATIS L.

    Directory of Open Access Journals (Sweden)

    Valentin CODREANU

    2016-02-01

    Full Text Available Tipul morfologic anomocit al aparatelor (complexelor stomatice caracterizează epiderma abaxială şi adaxială a laminei frunzei la 5 specii de Clematis L. Densitatea stomatelor este specifică şi variază, în anul secetos 2007, de la 119,05 stomate/mm2 la specia Clematis vitalba până la 182,05 stomate/mm2 la specia C.tangutica.Grosimea medie mai mare a laminei frunzei şi prezenţa stomatelor în epiderma adaxială a laminei frunzei eviden­ţiază speciile mai rezistente la secetă. CANTITATIVE ANATOMY OF LEAF BLADE OF SOME CLEMATIS L. SPECIESAs a result of quantitative anatomic study of leaf blade of 5 species of genus Clematis L., is established that anomocytic morphological type of stomatal apparatus (complex is characteristic for the abaxial and adaxial epidermis. Stomatal density of leaf epidermis of clematises is specific, but varies in different years. The density of leaf stomata characterizes every species and varies, in drought 2007 year, from 119,05/mm2 at species Clematis vitalba to 182,05 stomata/mm2, at species C.tangutica. The greater average thickness of leaf blade and present of stomata in adaxial epidermis of leaf blade evidence more drought resistant species of clematises.

  9. Patrones asociados a la conducta de desplazamiento local en Phymactis clematis Drayton (Anthozoa: Actiniidae Patterns of local movement behavior in Phymactis clematis Drayton (Anthozoa: Actiniidae

    Directory of Open Access Journals (Sweden)

    MARCELO M. RIVADENEIRA

    2001-12-01

    Full Text Available Phymactis clematis Drayton es la especie de actinia más abundante y unas de las que alcanza mayor tamaño en la costa de Sudamérica. Al igual que en otras especies de actinias, los adultos de la especie poseerían una activa capacidad de realizar desplazamientos locales. Sin embargo estos no han sido adecuadamente descritos, desconociéndose los factores causales. En este trabajo pretendemos describir algunos patrones básicos asociados a la conducta de desplazamiento local en P. clematis, evaluados indirectamente a partir de la presencia de halos de roca desnuda alrededor de los individuos en dos sitios intermareales rocosos en la costa del norte de Chile (Cavancha y Colorado Chico. Los desplazamientos fueron más frecuentes y de mayor intensidad en Colorado Chico, donde más del 50 % de los individuos evidenciaron desplazamientos, aunque generalmente no superaron los 60 mm de extensión. En Colorado Chico, los desplazamientos ocurrieron principalmente en individuos con diámetro pedal superior a 40 mm, mientras que la intensidad de éstos decrecería con el tamaño. Discutimos que las causas del mayor desplazamiento de individuos en Colorado Chico podrían estar asociadas a la mayor abundancia y menor espaciamiento entre los individuos, los cuales derivarían en una interferencia física entre los individuos. De este modo sugerimos que los patrones asociados al tamaño de los individuos emergerían secundariamente, siendo supeditados al nivel de abundancia local. A pesar de que los desplazamientos de P. clematis en Colorado Chico ocurren en general a una escala de unos pocos centímetros, tendrían un efecto significativo en el espaciamiento interindividual, incrementando en promedio la distancia al vecino más próximo. Se plantea la posibilidad de que los desplazamientos locales serían un mecanismo efectivo de reducción de la competencia intraespecífica en P. clematisPhymactis clematis Drayton is the most abundant and one of the largest

  10. ANTIMICROBIAL ACTIVITY OF CLEMATIS BRACHIATA THUNB LEAF EXTRACTS

    Directory of Open Access Journals (Sweden)

    M. Mostafa* and A.J. Afolayan

    2013-01-01

    Full Text Available The antimicrobial activity as well as phytochemical screening of the hexane, acetone, methanol and water extracts of Clematis brachiata Thunb (Ranunculaceae leaves was investigated. The agar dilution assay method was used for the evaluation of the antimicrobial activity of extracts against 10 bacteria and four fungal species. The phytochemical screenings were performed by using the standard procedures. The acetone and methanol extracts were active against the 10 bacteria strains with MIC ranging between 1.0 and 3.0 mg/ml, whereas the water extract was only active against two Gram-negative bacterial strains at 10.0 mg/ml. There was no activity from the hexane extract. While there was complete growth inhibition by the acetone and methanol extracts against all the fungal species at 10 mg/ml, the hexane extract was active against all the fungal species except Candida albicans at 10 mg/ml. In contrast, the water extract did not show any activity against the fungal species. Phytochemical screening revealed the presence of phenols, tannin, saponin, flavonoids, terpenoids and glycosidic compounds and could be responsible for the above activities of the extracts. The results of this study support the traditional uses of this plant as antibiotics.

  11. 铁线莲属威灵仙组修订%A revision of Clematis sect.Clematis(Ranunculaceae)

    Institute of Scientific and Technical Information of China (English)

    王文采

    2003-01-01

    对铁线莲属铁线莲亚属Clematis subgen. Clematis中的欧洲铁线莲C. vitalba L.演化干的原始群威灵仙组sect. Clematis进行了全面修订,确定此组共含有73种和45变种.写出了威灵仙组的分类学简史及地理分布;对威灵仙组中各亚组的亲缘和主要区别特征以及铁线莲亚属欧洲铁线莲演化干中各群的亲缘关系进行了讨论.将威灵仙组分为5个亚组,写出了分亚组检索表和各亚组的分种检索表,以及各种植物的形态描述、地理分布、生长环境等,并附有多幅插图.在5亚组中,欧洲铁线莲亚组(钝萼铁线莲亚组) subsect. Clematis为此组的原始群,其主要特征:藤本;小叶通常草质或纸质,通常边缘具齿,有时全缘;萼片4,外面的毛长1 mm以下;花药长圆形、椭圆形或狭长圆形,稀条形,药隔顶端不突起,稀稍突起.此亚组的瘦果两侧扁压,但多数不扁平,不具边缘,只在短毛铁线莲C. puberula Hook. f. & Thoms.强烈扁压,扁平,周围具宽边缘.在铁线莲属中,除短毛铁线莲外,特产朝鲜的C. brachyura Maxim. (sect. Pterocarpa Tamura)也具有近似这种进化类型的瘦果.铁线莲组的其他4个亚组各含有较进化的特征,可能均自欧洲铁线莲亚组演化而出:单种的厚叶铁线莲亚组subsect. Crassifoliae (Tamura) Tamura为藤本;小叶革质,全缘,很像威灵仙亚组的小叶;雄蕊花丝皱缩,花药宽长圆形或长圆形.单种的长毛铁线莲亚组subsect. Baominianae (W. T. Wang) W. T. Wang为藤本;小叶纸质,具齿;花大,具6枚萼片;萼片外面的毛长1.6-3 mm; 花药长圆形.棉团铁线莲亚组subsect. Angustifoliae的主要特征:茎直立;花常具5-6枚萼片.威灵仙亚组subsect. Rectae Prantl的主要特征:藤本,稀茎直立;小叶常革质,全缘,稀具齿;萼片通常4枚,稀较多,外面的毛长在1 mm以下;花药条形,有时狭长圆形,药隔顶端常突起.此亚组的威灵仙系ser. Rectae Prantl具有进化类型的

  12. Triterpene saponins from Clematis chinensis and their potential anti-inflammatory activity.

    Science.gov (United States)

    Fu, Qiang; Zan, Ke; Zhao, Mingbo; Zhou, Sixiang; Shi, Shepo; Jiang, Yong; Tu, Pengfei

    2010-07-23

    Seven new triterpene saponins, clematochinenosides A-G (1-7), together with 17 known saponins (8-24), were isolated from the roots and rhizomes of Clematis chinensis. Their structures were elucidated on the basis of spectroscopic evidence and hydrolysis products. Compounds 1, 3-7, and 20-24 showed inhibitory activities against COX-1 and COX-2 enzymes. PMID:20540535

  13. Antioxidant and Anti-Inflammatory Phenolic Glycosides from Clematis tashiroi.

    Science.gov (United States)

    Zhang, Li-Jie; Huang, Hung-Tse; Huang, Shih-Yen; Lin, Zhi-Hu; Shen, Chien-Chang; Tsai, Wei-Jern; Kuo, Yao-Haur

    2015-07-24

    From the 95% EtOH extract of dried aerial parts of Clematis tashiroi, eight new and four known phenolic (caffeic acid, coumaric acid, ferrulic acid) glycosides were isolated and characterized. The structures of the new isolates (clematisides A-H) were elucidated by spectroscopic data interpretation as trans-4-O-(6-O-trans-caffeoyl-β-D- glucopyranosyl)-9-O-β-D-glucopyranosyl caffeic acid (1), trans-4-O-(6-O-trans-feruloyl-β-D-glucopyranosyll)-9-O-β-D-glucopyranosyl caffeic acid (2), trans-4-O-(6-O-trans-p-coumaroyl-β-D-glucopyranosyl)-9-O-β-D-glucopyranosyl caffeic acid (3), trans-4-O-(6-O-trans-caffeoyl-β-D-glucopyranosyl)-9-O-β-D-glucopyranosyl p-coumaric acid (4), trans-3-O-(6-O-trans-caffeoyl-β-D-glucopyranosyl)-9-O-β-D-glucopyranosyl caffeic acid (5), trans-3-O-(6-O-trans-p-coumaroyl-β-D-glucopyranosyl)-9-O-β-D-glucopyranosyl caffeic acid (6), 6-(3',4'-dihydroxystyryl)-2-pyrone-4-O-(6-O-trans-caffeoyl)-β-D-glucopyranoside (7), and 6-(3',4'-dihydroxystyryl)-2-pyrone-4-O-{6-O-[4-O-(6-O-trans-caffeoyl)-β-D-glucopyranosyl]-trans-caffeoyl}-β-D-glucopyranoside (8), respectively. In a DPPH radical-scavenging test, compounds 1, 7, and 8 showed more potent antioxidant activity than that of the positive control, vitamin E. In addition, compound 7 also showed inhibitory activity in an antinitric oxide release assay. PMID:26143931

  14. 中药材威灵仙的研究进展%Research progress of Clematis chinensis Osbeck

    Institute of Scientific and Technical Information of China (English)

    典灵辉

    2004-01-01

    威灵仙,又称粉灵仙、灵仙、黑须公、铁灵仙等,为毛茛科铁线莲属威灵仙(Clematis chinensis Osbeck)的干燥根茎,属多年生草本植物。据《中国药典》记载威灵仙(Clematis chinensis Osbeck)、棉团铁线莲(Clematis hexapetala Pall.)或东北铁线莲(Clematis manshurica Rupr.)为正品。威灵仙味辛成,性温,有微毒,具有祛风除湿,通络止痛之功效。临床上主要用于风湿痹痛,肢体麻木,筋脉挛急,屈伸不利等症状。

  15. Growth Habit and Mechanical Architecture of the Sand Dune‐adapted Climber Clematis flammula var. maritima L.

    OpenAIRE

    Isnard, Sandrine; Rowe, Nick; Speck, Thomas

    2003-01-01

    Clematis flammula var. maritima is a woody lianoid plant that grows on coastal sand dunes in the Mediterranean region. Older perennial stems are present as extensive underground axes. These generate surface growth of shorter‐lived stems producing monospecific trellises above the surface of the sand. Despite its sand dune habitat and shortage of host support plants, this variety of Clematis shows mechanical characteristics during growth that are closely comparable with those of scandent woody ...

  16. ANATOMIA CANTITATIVĂ A LAMINEI FRUNZEI LA UNELE SPECII DE CLEMATIS L.

    OpenAIRE

    Valentin CODREANU

    2016-01-01

    Tipul morfologic anomocit al aparatelor (complexelor) stomatice caracterizează epiderma abaxială şi adaxială a laminei frunzei la 5 specii de Clematis L. Densitatea stomatelor este specifică şi variază, în anul secetos 2007, de la 119,05 stomate/mm2 la specia Clematis vitalba până la 182,05 stomate/mm2 la specia C.tangutica.Grosimea medie mai mare a laminei frunzei şi prezenţa stomatelor în epiderma adaxială a laminei frunzei eviden­ţiază speciile mai rezistente la secetă. CANTITATIVE ANATOMY...

  17. Odlingsvärda vildarter inom släktet Clematis

    OpenAIRE

    Götmark, Hulda

    2012-01-01

    Clematis har länge varit älskade trädgårdsväxter och är även idag mycket populära. Kombinationen av ett vackert växtsätt och en överdådig, ofta långvarig blomning gör att de lämpar sig mycket bra som prydnadsväxer. Clematis-släktet omfattar cirka 300 arter och tillhör familjen Ranunculaceae. Arterna förekommer i alla världsdelar, men återfinns mestadels i tempererade områden. På trädgårdsmarknaden finns idag tusentals sorter och kulturhybrider, men vildarter är ovanligare som prydnadsväxter. ...

  18. Use of Plant Growth Regulators to Increase Branching of Clematis Spp.

    OpenAIRE

    Puglisi, Sadie Erica

    2002-01-01

    Clematis spp. L. is a twining vine covered in showy blooms. Typical growth of hybrids is from the main leader, producing a thin, unbranched plant with one cyme. Apical dominance is released by cutting back the vine during production. Cutting back, or pinching, of a plant is labor intensive and compromises bloom for vegetative growth at time of sales. The purpose of this project was to eliminate manual pinching by treating young plants with chemical plant growth regulators (PGRs) that enha...

  19. In vitro antimicrobial activity and phytochemical screening of clematis species indigenous to Ethiopia

    Directory of Open Access Journals (Sweden)

    S Hawaze

    2012-01-01

    Full Text Available The leaves extracts of two indigenous plants of Ethiopia: Clematis longicauda steud ex A. Rich. and Clematis burgensis Engl. are used in Southwestern Ethiopia to treat otorrhoea and eczema. Antimicrobial activity and MIC of crude extracts were determined by disk diffusion and broth dilution. Phytochemical screening was performed on the extracts. The methanol and petroleum ether extracts of both plants showed antibacterial and antifungal activity. Sensitivity of reference strains was concentration dependent. Methanol and petroleum ether extracts of C. burgensis leaves exerted greater inhibitory effects than C. longicauda extracts whereas aqueous extracts of both plants were inactive. The MIC study revealed a concentration of 0.78 mg/ml on bacteria and 3.125 mg/ml on fungi for methanol extract and 1.56 mg/ml on both fungi and bacteria for petroleum ether extract. Phytochemical screening results indicated the presence of proteins, fixed oils, carbohydrates, tannins, saponins, flavonoids, and steroids. Preliminary chromatographic investigation showed fluorescing spots with R f values that ranged from 0.05 to 0.96 for phenolic compounds and saponins. As the study is one of the first reports on the two indigenous species of Clematis; isolation, purification and characterization of the different primary and secondary metabolites may further yield alternative options to the microbial chemotherapy.

  20. In vitro Antimicrobial Activity and Phytochemical Screening of Clematis Species Indigenous to Ethiopia.

    Science.gov (United States)

    Hawaze, S; Deti, H; Suleman, S

    2012-01-01

    THE LEAVES EXTRACTS OF TWO INDIGENOUS PLANTS OF ETHIOPIA: Clematis longicauda steud ex A. Rich. and Clematis burgensis Engl. are used in Southwestern Ethiopia to treat otorrhoea and eczema. Antimicrobial activity and MIC of crude extracts were determined by disk diffusion and broth dilution. Phytochemical screening was performed on the extracts. The methanol and petroleum ether extracts of both plants showed antibacterial and antifungal activity. Sensitivity of reference strains was concentration dependent. Methanol and petroleum ether extracts of C. burgensis leaves exerted greater inhibitory effects than C. longicauda extracts whereas aqueous extracts of both plants were inactive. The MIC study revealed a concentration of 0.78 mg/ml on bacteria and 3.125 mg/ml on fungi for methanol extract and 1.56 mg/ml on both fungi and bacteria for petroleum ether extract. Phytochemical screening results indicated the presence of proteins, fixed oils, carbohydrates, tannins, saponins, flavonoids, and steroids. Preliminary chromatographic investigation showed fluorescing spots with R(f) values that ranged from 0.05 to 0.96 for phenolic compounds and saponins. As the study is one of the first reports on the two indigenous species of Clematis; isolation, purification and characterization of the different primary and secondary metabolites may further yield alternative options to the microbial chemotherapy. PMID:23204619

  1. Research Progress on Clematis Medicinal Plants%铁线莲属药用植物的研究进展

    Institute of Scientific and Technical Information of China (English)

    刘慧; 张钦德

    2012-01-01

    The paper reports the research progress on Clematis medicinal plants in every province, and summarizes the chemical constituents and pharmacological effects of Clematis medicinal plants, reports the research situation of Clematis plants in Shandong Province and gives suggestions for their rational development and utilization.%综述了铁线莲属药用植物在各省的研究进展,总结了铁线莲属植物的化学成分及药理作用,并对山东省铁线莲属植物的研究现状进行了研究,为该属资源合理利用提供了依据.

  2. 单叶铁线莲Clematis henryi愈伤组织诱导与植株再生%Callus induction and plant regeneration of Clematis henryi

    Institute of Scientific and Technical Information of China (English)

    黄余磊; 吕枷薪; 蒋明; 李温平; 张徐俞; 邹清成

    2011-01-01

    To develop a procedure for callus induction and plant regeneration of Clematis henryi, experiments were performed on Murashige and Skoog medium supplemented with different hormones using old vines, tender stems, leaves and leafstalks as explants. The results showed that tender stems were the best explants for differentiating callus, and gave the highest induction rate of 88. 6% on MS + 1. 2 mg·L-1 6-BA +0. 35 mg·L-1 NAA. MS +4.0 mg ·L -1 KT+0.4 mg·L-1 NAA, which was proved to be the best adventitious shoots formation medium with a induction rate of 90.0%. The best rooting medium was obtained while Clematis henryi was cultured on MS + 0. 04 mg· L -1 NAA, resulting in a rooting percentage of 95. 0%.%以单叶铁线莲老枝、嫩茎、叶片和叶柄为外植体,在附加各种激素的MS培养基上进行诱导培养,以探讨愈伤组织诱导和植株再生的条件.结果表明,嫩茎为最佳外植体,适用于愈伤组织的诱导,在MS+ 1.2 mg·L -1 6-BA+0.35 mg·L -1 NAA培养基中,嫩茎的出愈率为88.6%;MS +4.0 mg·L-1 KT +0.4 mg·L-1 NAA 为最佳不定芽诱导培养基,不定芽发生率为90.0%;MS +0.04 mg·L-1 NAA为最佳生根培养基,生根率达95.0%.

  3. Optimization of polysaccharides extraction from Clematis huchouensis Tamura and its antioxidant activity.

    Science.gov (United States)

    Zhang, Zhongshan; Wang, Xiaomei; Zhao, Mingxing; Qian, Kun

    2014-10-13

    In this study, response surface methodology (RSM), based on Box-Behnken design, was employed to optimize the extraction conditions of polysaccharide from Clematis huchouensis Tamura (CP). And then the antioxidant activities of the samples were investigated including scavenging effects of superoxide and hydroxyl radicals and their reducing power. The results of chemical composition and FT-IR spectrum analysis showed the polysaccharide was acidic proteoglycan. And moreover, CP showed excellent antioxidant activity in these three assays. The purification and structure of CP need to be further studied. PMID:25037413

  4. Triterpenoid saponins with anti-myocardial ischemia activity from the whole plants of Clematis tangutica.

    Science.gov (United States)

    Zhang, Wei; Yao, Min-Na; Tang, Hai-Feng; Tian, Xiang-Rong; Wang, Min-Chang; Ji, Lan-Ju; Xi, Miao-Miao

    2013-05-01

    Four new triterpenoid saponins named clematangosides A-D (1-4) along with six known saponins (5-10) were isolated from the whole plants of Clematis tangutica. Their structures were determined by extensive spectral analysis and chemical evidences. All saponins were evaluated for their protective effects in hypoxia-induced myocardial injury model. Compounds 2-4, 6, and 10 exhibited anti-myocardial ischemia activities with ED50 values in the range of 75.77-127.22 µM. PMID:23670628

  5. A new indole alkaloidal glucoside from the aerial parts of Clematis terniflora DC.

    Science.gov (United States)

    Li, Wen-Ting; Yang, Bing-Xian; Zhu, Wei; Gong, Ming-Hua; Xu, Xiang-Dong; Lu, Xiang-Hong; Sun, Lian-Li; Tian, Jing-Kui; Zhang, Lin

    2013-01-01

    A new indole alkaloidal glucoside together with three known compounds aurantiamide acetate (2), eleutheroside E (3) and 1-O-caffeoyl-β-D-glucopyranoside (4) has been isolated from ethanol extract of the aerial parts of Clematis terniflora DC. On the basis of their spectroscopic and chemical evidence, the new compound was elucidated as (6-O-β-D-glucopyranosyl-1H-indol-3-yl) carboxylic acid methyl ester (1). Compounds 1 and 3 showed significant cytotoxicity against human ECA-109. PMID:24050211

  6. Photometry of 683 Lanzia, 1101 Clematis, 1499 Pori, 1507 Vaasa, and 3893 DeLaeter

    Science.gov (United States)

    Stephens, Robert D.

    2004-03-01

    Results for the following asteroids (lightcurve period and amplitude) observed from Santana Observatory during the period July to September 2003 are reported: 683 Lanzia (8.63 ± 0.005 hours and 0.15 mag.), 1101 Clematis (12.68 ± 0.01 hours and 0.40 mag.), 1499 Pori (3.36 ± 0.01 hours and 0.28 mag.), 1507 Vaasa (34.07 ± 0.01 hours and 0.24 mag.), 3893 DeLaeter (13.83 ± 0.01 hours and 0.33 mag.).

  7. Plant Regeneration from Callus Culture of Clematis gouriana Roxb. – A Rare Medicinal Plant

    OpenAIRE

    NAIKA, Hanumanaika RAJA; KRISHNA, Venkatarangaiah

    2008-01-01

    An in vitro regeneration protocol through stem callus culture has been standardized for the medicinal climber Clematis gouriana. The explant induced callus on MS-medium supplemented with 0.5 to 1.5 mg l-1 BAP and 0.1 to 0.5 mg l-1 NAA. The optimized callus induction occurred at the concentration of 1.0 mg l-1 BAP and 0.3 mg l-1 NAA. After initiation of callus, it was immediately transferred to MS medium containing 4.0 mg l-1 FAP and 0.5 mg l-1 indole-3-butyric acid (IBA). Upon longer incubati...

  8. 铁线莲属研究随记(Ⅵ)%Notes on the genus Clematis (Ranunculaceae) (Ⅵ)

    Institute of Scientific and Technical Information of China (English)

    王文采

    2006-01-01

    In this paper, the status of Clematis eriopoda Maxim. and sect. Atragenopsis Boiss. is discussed, and the species status of the former and the sectional status of the latter are recognized; two species (C. wenxianensis W. T. Wang, C. malacoclada W. T. Wang) and one variety (C. hastata Finet & Gagnep. var. micrantha W. T. Wang) are described as new;one species, C. tubulosa Turcz., which has been erroneously relegated into the synonymy under C. heracleifolia DC. by Fang and Wang for a long time, is reinstated; C. heracleifolia var. ichangensis Rehd. & Wils. is transferred to C. tubulosa, and a new combination, C.tubulosa var. ichangensis (Rehd. & Wils.) W. T. Wang, is made; the fruit description of C.glabrifolia K. Sun & M. S. Yan is given for the first time.%讨论了Clematis eriopoda Maxim.和sect.Atragenopsis Boiss.的地位,认为这二分类群均应成立;描述了2新种,1新变种;过去长期被归并的卷萼铁线莲C.tubulosa得到恢复;Clematis heracleifolia var.ichangensis被转移改作卷萼铁线莲的变种;首次给出光叶铁线莲Clematis glabrifolia的果实的形态描述.

  9. Wound healing activity of the methanol extracts of Clematis species indigenous to Ethiopia

    Directory of Open Access Journals (Sweden)

    Segewkal Hawaze

    2013-01-01

    Full Text Available Objective: To investigate the vulnerary activity of the methanol extracts of Clematis longicauda steud ex A. Rich, and Clematis burgensis Engl leaves. Materials and Methods: In vivo wound healing activity of methanol extracts of C. longicauda and C. burgensis were studied using excision wound model, re-sutured incision wound model, histopathological analysis and anti-inflammatory activity test. The variables studied were percentage of wound contraction and epithelialisation period in excision wound model; tensile strength in incision wound model; and percentage of acetic-acid-induced capillary permeability inhibition in the anti-inflammatory activity test. The histopathological study qualitatively assessed presence of inflammatory cells, fibroblast proliferation, collagen formation and angiogenesis. Differences between experimental groups were compared by one-way analysis of variance, followed by Dunnett′s test. P < 0.0001 was considered statistically significant. Results: C. longicauda and C. burgensis extracts treated animals showed significant reduction in wound area and faster rate of epithelialisation, P < 0.0001. Extracts of these plants also demonstrated statistically significant wound breaking strength and inhibition of vascular permeability induced by acetic acid. Histological studies on granulation tissue sections showed formation of collagen bundles in the C. longicauda and C. burgensis extracts treated and standard drug-treated groups while inflammatory cells were present in control. Conclusion: Methanol extracts of C. longicauda and C. burgensis had comparable wound healing activity to Madecassol, containing 1% of Centalla asiatica extracts.

  10. New acylated anthocyanins and other flavonoids from the red flowers of Clematis cultivars.

    Science.gov (United States)

    Hashimoto, Masanori; Suzuki, Toshisada; Iwashina, Tsukasa

    2011-11-01

    Six new acylated cyanidin glycosides, cyanidin 3-O-beta-(2''-E-caffeoylglucopyranosyl)-(1 --> 2)-O-beta-galactopyranoside (1), cyanidin 3-O-beta-(2''-E-caffeoylglucopyranosyl)-(1 --> 2)-O-beta-(6''-malonylgalactopyranoside) (2), cyanidin 3-O-beta-(2''-E-caffeoylglucopyranosyl)-(1 --> 2)-O-beta-(6''-succinylgalactopyranoside) (3), cyanidin 3-O-beta-(2''-E-caffeoylglucopyranosyl)-(1 --> 2)-O-beta-galactopyranoside-3''- O-beta-glucuronopyranoside (4), cyanidin 3-O-beta-(2''-E-caffeoylglucopyranosyl)-(1 --> 2)-O-beta-(6''-malonylgalactopyranoside)-3'-O-beta-glucuronopyranoside (5), and cyanidin 3-O-beta-(2'-E-feruloylglucopyranosyl)-(1 --> 2)-O-beta-(6''-malonylgalactoside)-3' -O-beta-glucuronopyranoside (6), were isolated from the red flowers of two Clematis cultivars, 'Niobe'and 'Madame Julia Correvon'. The chemical structures of the isolated anthocyanins were determined by UV, LC-MS, HPLC, TLC, characterization of hydrolysates, and 1H and 13C NMR spectroscopy, including H-H COSY, C-H COSY, HMBC, HMQC and NOESY. The last three anthocyanins were widely distributed in 37 red flower Clematis cultivars. On the other hand, the first three compounds were found only in two cultivars. Five known flavonol glycosides, kaempferol 3-O-glucoside, kaempferol 3-O-rutinoside, quercetin 3-O-galactoside, quercetin 3-O-glucoside and quercetin 3-O-rutinoside, were isolated from the flowers of'Madame Julia Correvon'. PMID:22224277

  11. Two new cytotoxic triterpenoid saponins from the roots of Clematis argentilucida.

    Science.gov (United States)

    Hai, Wenli; Cheng, Hua; Zhao, Mei; Wang, Yi; Hong, Liangjian; Tang, Haifeng; Tian, Xiangrong

    2012-06-01

    Bioassay-guided fractionation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of two new triterpenoid saponins along with a known one, cussonside B (3). By extensive spectral analysis and chemical evidences, the structures of the two new saponins were determined to be 3β-O-[β-D-ribopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl] hederagenin-11,13-dien-28-oic acid (1) and 3β-O-{β-D-ribopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-xylopyranosyl} oleanolic acid (2), respectively. Saponin 1 is the first example of triterpenoid saponins with two double bonds located at C-11 and C-13 in the aglycone from the genus Clematis. The two new saponins exhibited significant cytotoxicity against human leukemia HL-60 cell lines, human hepatocellular carcinoma Hep-G2 cell lines and human glioblastoma U251MG cell lines with a range of IC(50) values from 2.74 to 25.40μM, while 3 showed inactivity against all of the three cancer cell lines. PMID:22433396

  12. 2-de protocol optimization and evaluation for proteome analysis of genus clematis taxa (ranunculaceae)

    International Nuclear Information System (INIS)

    An approach was conducted to optimize two-dimensional gel electrophoresis (2-DE) method for leaf proteome analysis of genus Clematis species, as a molecular approach to explore its taxonomy and differentially expressed genome patterns. During establishment and optimization of protocol we extracted proteins by three extraction protocols, viz., phenol-SDS (PS) method, TCA/acetone (TA) method and lysis buffer (LB) method, and PS was the best one with 2.35, 0.05 micro g protein yield. For protein solubilization two lysis buffers (LB-1 and LB-2) were prepared, used and comparatively LB1 depicted better resolution. Proteins were by quantified by the Bio-Rad protein assay (Hercules, CA, USA) with bovine serum albumin as standard and purified by 2-D clean-up Kit (Amersham Biosciences). 2-DE analysis was conducted on pH 3-10, non-linear gradient strips (24cm) as first step, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on 13% polyacrylamide gels as the second phase. For spot visualization gels were stained for with silver stain. The gels were scanned using Powerlook 2100XL scanner and gel images were analyzed by ImageMaster 2-D Platinum. Validation of experiment was performed by measuring analytical variance (AV) and biological variance (BV) for replicate spots. AV was calculated for 60 protein spots present in three replicate 2-DE gels of the same protein extract and BV was determined for the same protein spots from independent tissue extracts corresponding to leaves from different plants, or the same tree at different orientations or sampling times during a day. Values of 26% for the analytical variance and 58.6% for the biological variance among independent sampled species were obtained.This provided a threshold values for the evaluation of protein expression changes in comparative proteomic investigations with this species. Some spots were selected and subjected to liquid chromatography mass spectrometry (LC-MS) for identification purpose. Due

  13. Triterpenoid saponins from the roots of Clematis argentilucida and their cytotoxic activity.

    Science.gov (United States)

    Zhao, Mei; Ma, Ning; Qiu, Feng; Hai, Wen-Li; Tang, Hai-Feng; Zhang, Yan; Wen, Ai-Dong

    2014-07-01

    The reinvestigation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of four new oleanane-type triterpenoid saponins, 1-4, four known saponins, 5-8, first isolated from the species, together with ten saponins, 9-18, reported in the preceding papers. The structures of saponins 1-8 were elucidated by extensive spectroscopic analysis and chemical evidences. The cytotoxicity of all the saponins were evaluated against human tumor HL-60, HepG-2, and SGC-7901 cell lines. The monodesmosidic saponins 4, 7, 8, and 14-18 exhibited cytotoxic activity against the three cell lines with IC50 values in the range of 0.87-19.48 µM. PMID:25029176

  14. Studies on the Chemical Components of Clematis chinensis%威灵仙化学成分研究

    Institute of Scientific and Technical Information of China (English)

    何明; 张静华; 胡昌奇

    2001-01-01

    Two novel compounds, clemaphenol A and dihydro-4-hydroxy-5-hyroxymethyl-2(3H)-furanone were isolated together with eight known compounds, isoferulic acid, β-sitosterol, daucosterol, 5-hydroxymethyl-2-furancarboxaldehyde, 5-hydroxy-4-oxo-pentanoic acid, palmitic acid, linoleic acid and anemonin from the root of Clematis chinensis Osbeck.%从毛莨科植物威灵仙Clematis chinensis Osbeck的根中分得两个新化合物-clemaphenol A(I)和二氢-4-羟基-5-羟甲基-2(3H)-呋喃酮.同时,还分得八个已知化合物,异阿魏酸,β-谷甾醇,胡萝卜甙,5-羟甲基呋喃甲醛,5-羟基乙酰丙酸,棕榈酸,亚油酸和白头翁素.

  15. Pharmacognostic Identification of Clematis florida var. plena%畲药十二时辰的生药鉴定

    Institute of Scientific and Technical Information of China (English)

    黄泽豪; 张月玲; 沈贤娟

    2013-01-01

      十二时辰是具福建民间特色的一味畲药,来源于毛茛科植物重瓣铁线莲Clematis florida var. plena的根。本文对十二时辰的植物形态、生药性状、组织构造、显微粉末特征进行观察,为该药的正确鉴别以及进一步研究、开发应用提供参考。%  The roots of Clematis florida var. plena (Ranunculaceae) were usually used as an ethnic drug in Fujian province. In this paper, we described the morphology, pharmaceutical character and microscopic characteristics of this ethnic medicine to identify it and provide foundation for its further study and application.

  16. Anti-inflammatory, antinociceptive and antipyretic properties of the aqueous extract of Clematis brachiata leaf in male rats.

    Science.gov (United States)

    Mostafa, Mohammad; Appidi, Jaipal R; Yakubu, Musa T; Afolayan, Anthony J

    2010-06-01

    Clematis brachiata Thunb. (Ranunculaceae) is used as a folk remedy for the treatment of pain, fever and inflammatory ailments. Aqueous extract of Clematis brachiata leaf was screened for its phytochemical constituents. The anti-inflammatory investigations were carried out using carrageenan and histamine-induced edema models; acetic acid writhing, formalin-induced pain and tail immersion models were used to evaluate antinociceptive activity while a Brewer's yeast-induced hyperthermia model was employed for the antipyretic experiment. Phytochemical screening of the extract revealed the presence of tannins, saponins, flavonoids and cardiac glycosides. The extract at 100, 200 and 400 mg/kg body weight significantly (PClematis brachiata leaves can be employed in the management of inflammation, pain and fever. These activities may be due in part to the flavonoid content of the extract. PMID:20645742

  17. 绣球藤的化学成分研究%Studies on the Chemical Composition of Clematis Montana

    Institute of Scientific and Technical Information of China (English)

    石亚囡; 许丰丹; 杨泰然

    2008-01-01

    从毛茛科铁线莲属植物绣球藤(Clematis montana Buch-Ham.)的干燥藤茎中分离得到两个化合物.根据化合物的波谱数据鉴定这两个化合物分别为(+)lariciresinol-di-4-O-B-D-glu和α-D-葡萄糖.

  18. Morphological and functional study of the marginal sphincter of the sea anemones Phymactis clematis and Aulactinia marplatensis from intertidal of Mar del Plata, Argentina Estudio morfológico y funcional del esfínter marginal de las anémonas de mar Phymactis clematis y Aulactinia marplatensis del intermareal de Mar del Plata, Argentina

    OpenAIRE

    Elvira A. González Oliveira; Darlo Luis Patronelli; Mauricio O. Zamponi

    2009-01-01

    It was made the characterization of marginal sphincter to the species Phymactis clematis (Drayton in Dana, 1849) and Aulactinia marplatensis (Zamponi, 1977), from intertidal ecosystem through their morphogical and functional study. The species P. clematis has a circumscript sphincter of palmate type. This muscle is constituted by a mesogloeal axis and several mesogloeal subaxes. Axis as well as subaxes give a support to the endoderm which border is smooth. Aulactinia marplatensis has a circun...

  19. A revision of Clematis sect.Aspidanthera s.l.( Ranunculaceae)%铁线莲属单性铁线莲组修订

    Institute of Scientific and Technical Information of China (English)

    王文采

    2004-01-01

    In this paper Clematis sect. Aspidanthera s. 1. is revised. Seventy-two species and 15 varieties are recognized. They are keyed, described, and in most cases illustrated, and are classified into six subsections. Of them, one series, five species, and two varieties are described as new,and two new ranks are proposed. Brief taxonomic history and geographical distribution of the section are given, the evolutionary trends of several important morphological characters observed are enumerated, and the relationships of its subsections are discussed. As a result, subsect. Dioicae,which shows striking resemblance in both habit and floral structure to sect. Clematis subsect.Clematis, is regarded as the primitive group, while subsect. Insidiosae, bearing pistillate flower with erect sepals and no staminodes, is regarded as the advanced group within the section.

  20. Clematis austrogansuensis, a new species of Ranunculaceae from Gansu%甘南铁线莲, 甘肃毛茛科一新种

    Institute of Scientific and Technical Information of China (English)

    王文采; 李良千

    2011-01-01

    A new species of Ranunculaceae, Clematis austrogansuensis W. T. Wang & L. Q. Li, is described from southern Gansu Province. In the genus Clematis,it is related to C. shensiensis W. T. Wang,from which it differs in its hornotinous branchlets only spasely puberulous on nodes, leaflets only near base sparsely puberulous, I-flowered cymes, and anthers with obtuse apexes.%该文描述了自甘肃南部发现的毛茛科铁线莲属的一新种,甘南铁线莲.

  1. New cytotoxic triterpenoid saponins from the whole plant of Clematis lasiandra Maxim.

    Science.gov (United States)

    Tian, Xiangrong; Feng, Juntao; Tang, Haifeng; Zhao, Mei; Li, Yuan; Hai, Wenli; Zhang, Xing

    2013-10-01

    Four new oleanane type triterpenoid saponins (1-4) and three known saponins (5-7) were isolated from the whole plant of Clematis lasiandra Maxim. The structures of the four new compounds were elucidated as 3-O-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1 → 4)]-β-D-xylopyranosyl hederagenin (1), 3-O-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl oleanolic acid 28-O-β-D-glucopyranosyl ester (2), 3-O-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-β-D-xylopyranosyl hederagenin (3) and 3-O-β-D-ribopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1→4)]-α-L-arabinopyranosyl hederagenin (4) on the basis of extensive spectroscopic analysis and chemical evidence. Compounds 1-7 were evaluated for their cytotoxicity against human tumor cell lines HL-60, Hep-G2 and SGC-7901, and all of the evaluated saponins showed significant cytotoxicity to those three tumor cell lines with IC₅₀ in the range from 1.40 to 19.50 μmol/L except for compounds 2 and 6. PMID:23916579

  2. Triterpenoid Saponins from Clematis graveolens and Evaluation of their Insecticidal Activities.

    Science.gov (United States)

    Rattan, Rajeev; Reddy, S G Eswara; Dolma, Shudh Kirti; Fozdar, Bharat Inder; Gautam, Veena; Sharma, Ritika; Sharma, Upendra

    2015-09-01

    A new hederagenin based triterpenoid saponin, clematograveolenoside A (1), along with three known saponins, tomentoside A (2), huzhangoside D (3) and clematoside S (4), were isolated from the roots and rhizomes of Clematis graveolens. The structure of new compound was elucidated on the basis of detailed analysis of chemical and spectroscopic data including 1D- and 2D NMR spectra. Compound 2 was found the most effective against aphid (Aphis craccivora) with an LC50 of 1.2 and 0.5 mg/mL after treatment for 72 and 96 h, respectively and was followed by compound 4 (LC50 = 2.3 and 1.9 mg/mL) and 1 (LC50 = 3.2 and 2.6 mg/mL). In case of termite (Coptotermis homii), compound 1 was found more toxic with an LC50 of 0.1 mg/L after 24 h of treatment followed by compound 2, 3 and 4 (LC50 = 0.1, 0.2 and 0.2 mg/mL, respectively). PMID:26594749

  3. Screening of bioconstituents and in vitro cytotoxicity of Clematis gouriana leaves.

    Science.gov (United States)

    Rana, Shalika; Rawat, Kiran; Mahendru, Madhavi; Padwad, Yogendra; Pakade, Yogesh B; Lal, Brij; Bhushan, Shashi

    2015-01-01

    Clematis gouriana (Ranunculaceae), a perennial herb, is used by the local inhabitants of the western Himalayan region for its medicinal properties. Major bioconstituents of C. gouriana leaves using different solvent extracts were obtained and analysed. The results revealed promising contents of phenolics (from 18.19 ± 0.10 to 22.17 ± 0.10 mg g(-1)) as gallic acid and flavonoids (from 2.83 ± 0.01 to 6.52 ± 0.08 mg g(-1)) as quercetin equivalent in different extracts. Aqueous acetone extract showed higher antioxidant activity with IC50 value of 129.11 and 25.35 μg mL(-1) against DPPH and ABTS free radicals, respectively. Antioxidant yield ranged from 16.87 ± 0.27 to 24.48 ± 0.13 mg g(-1) of Trolox equivalent in different extracts as measured by the FRAP assay. Furthermore, ethylacetate extract exhibited strong in vitro cytotoxicity against Chinese hamster ovary and glioma cell lines. Proximate composition (proteins, fats, ash and minerals) of C. gouriana leaves was also assessed. Results demonstrated the potential of C. gouriana bioconstituents as nutraceuticals. PMID:25587822

  4. Nuevos datos sobre la asilvestrada Clematis orientalis L. (Ranunculaceae) en la Península Ibérica

    OpenAIRE

    Guara Requena, Miguel; Ferrer-Gallego, Pedro Pablo

    2006-01-01

    Se cita una nueva población del xenófito Clematis orientalis L. en las inmediaciones del área metropolitana de Teruel próxima a la ribera del río Turia. Este taxon junto con otras clemátides asiáticas como C. glauca Willdenow y C. tangutica (Maximowicz) Korshinsky, integran un complejo conjunto de táxones morfológicamente muy semejantes que quedan discriminados por sus tipos de inflorescencias, indumento del perianto y, la morfología e indumento de los foliolos. El proceso de expansión de est...

  5. Medicamentos herbarios en el centro-oeste argentino, V. Clematis montevidensis var. montevidensis, caracterización de la droga

    OpenAIRE

    Petenatti, Marta E.; Álvarez, María E; Petenatti, Elisa M.; Del Vitto, Luis A.; Saad, José R.; Mauricio R. Téves; Giordano, Oscar S.

    2005-01-01

    Clematis montevidensis Spreng. var. montevidensis (Ranunculaceae) es una liana denominada vulgarmente "barba de viejo", "cabello de ángel", "bejuco" o "loconte", empleada en medicina popular por sus propiedades rubefacientes hasta vesicantes en la región centro-oeste de la Argentina, donde está ampliamente distribuida. Ensayos recientes han demostrado que la droga ejerce fuerte acción irritante y moderada acción diurética. Para definir adecuadamente sus caracteres y contribuir al efectivo con...

  6. 东北铁线莲多糖提取及含量测定%Extraction Technique for the Polysaccharides from Clematis Manshurica Rupr

    Institute of Scientific and Technical Information of China (English)

    马琳; 李珊珊; 许世泉; 刘继永; 王志清; 杨振; 孙海; 张亚玉

    2014-01-01

    以多糖提取率、糖醛酸提取率为指标,比较水提取、酸提取和碱提取对多糖的提取效果,探索东北铁线莲多糖提取的最佳方法和工艺。分别以水、0.1mol/L盐酸、0.1mol/L氢氧化钠溶液、100℃水浴提取铁线莲多糖,苯酚-浓硫酸法测定东北铁线莲多糖含量,间羟基联苯法测定糖醛酸含量。结果,水提取、酸提取、碱提取东北铁线莲多糖含量分别为50.947%、29.064%、22.083%,糖醛酸含量分别为0.952%、38.903%、6.588%。水提取法的多糖提取率远远高于酸提取与碱提取,酸提取的糖醛酸提取率高于水提取与碱提取。%Extraction technique of polysaccharides from Clematis manshurica Rupr .was optimized .Compared with the extraction rate of polysac-charides and uronic acid by water ,acid and alkali .Methods :extraction of the polysaccharide from Clematis mandshurica Rupr .with 0.1mol/L HCL ,0.1mol/L NaOH and 100℃ water bath respectively .Determination of the contents of polysaccharide in Clematis mandshurica Rupr .phe-nol by sulfuric-acid method with the method of M -hydroxydiphenyl to Determination of the contents of polysaccharide in Clematis mandshuri-ca Rupr .As a result that the extracted contents of polysaccharides were 50.947% ,29.064% ,22.083% .and the extracted contents of uronic acid by acid solution were 0.952% 、38.903% 、6.588% in Clematis manshurica Rupr .The extraction rate of water is higher in polysaccharides from Clematis manshurica Rupr .and the extraction rate of uronic acid with acid solution is higher than water and alkali solution .The extraction technique operation is simple and good stability ,It is a well determination technique in the contents of polysaccharide in Clematis mandshuric Rupr .

  7. Chemical Constituents of Clematis montana%绣球藤的化学成分

    Institute of Scientific and Technical Information of China (English)

    宋成芝; 王跃虎; 华燕; 吴章康; 杜芝芝

    2008-01-01

    AIM: To study the chemical constituents of Clematis montana Buch. -Ham. ex DC.. METHODS: Aerial part of the plant wag extracted by ethanol firstly and then the ethyl acetate part of the ethanol extract Was isolated by different column chromatographic techniques including silica gel and Sephadex LH-20. Structures of these compounds were identified on the basis of spectroscopic analysis. RESULTS: Fourteen compounds were isolated and their structures were determined to be coniferaldehyde (1), caffeic acid (2), pluchoic acid (3), protocatechualdehyde (4), vanillin (5), hydroxytyrosol (6), 4-carbonyl-5-hydmxy methyl valerate (7), 4-hydroxydodec-2-enedioic acid (8), (+)-dihydrodehydrodiconiferyl alcohol (9), (-)-syringaresinol (10), (+)-guayarol (11), (-)-arctiin (12), (-)-lariciresinol (13), and hyperin (14). CONCLUSION: All the compounds were obtained from this plant for the first time.%目的:对绣球藤的化学成分进行研究.方法:利用硅胶柱色谱、Sephadex LH-20等方法进行分离纯化.根据理化性质和光谱分析进行结构鉴定.结果:从绣球藤乙醇提取物的乙酸乙酯部分中分离得到14个化合物,分别鉴定为:松柏醛(1),咖啡酸(2),pluchoic acid(3),原儿茶醛(4),香草醛(5),3,4-二羟基苯乙醇(6),4-羰基-5-羟基戊酸甲酯(7),4-hydroxydodec-2-enedioic acid (8),(+)-川木香醇F(9),(-)-丁香脂素(10),(+)-guayarol(11),(-)-牛蒡苷(12),(-)-落叶松树脂醇(13),金丝桃苷(14).结论:这些化合物均为首次从该植物中分离得到.

  8. 威灵仙化学成分的研究%STUDIES ON THE CHEMICAL COMPONENTS OF CLEMATIS CHINENSIS

    Institute of Scientific and Technical Information of China (English)

    何明; 张静华; 胡昌奇

    2001-01-01

    目的研究毛茛科植物威灵仙Clematis chinensis Osbeck根中的化学成分。方法利用硅胶柱色谱进行分离纯化,根据理化性质和光谱数据进行结构鉴定。结果得到两个化合物,分别鉴定为clemaphenol A (I)和二氢-4-羟基-5-羟甲基-2(3H)-呋喃酮(dihydro-4-hydroxy-5-hyroxymethyl-2(3H)-furanone,II)。结论 I,II均为新化合物。%AIM To study the chemical components of the root of Clematis chinensis. METHODS Various chromatographic techniques were used to separate and purify the components. Their structures were determined on the basis of spectral and chemical evidence. RESULTS Two compounds were isolated and elucidated as: clemaphenol A (I) and dihydro-4-hydroxy-5-hyroxymethyl-2(3H)-furanone (II). CONCLUSION Compound I and II are new compounds.

  9. Leaf proteome analysis of clematis chinensis: a traditional chinese medicine (tcm) by two-dimensional electrophoresis technique

    International Nuclear Information System (INIS)

    Leaf proteome of Clematis chinensis, a traditional Chinese medicine (TCM) was analyzed by two-dimensional electrophoresis (2-DE) technique. The samples were extracted by phenol-SDS method (PSM) with high protein quantity i.e. 2.35, 0.345 mg/g (yield/dw). Proteins were visualized by staining of gels by silver stain and CBB. The gel images of each species were compared by Image Master 2D Platinum software for analytical purpose. The 2-DE profile depicted distribution of 1085 spots and out of these only 255 protein spots (23.5%) were common to all analyzed taxa. The visualized protein spots showed pI range from 3.0 to 10.0 (pH) and Mr of 7 kDa to 70 kDa. Twelve proteins were exclusively specific to C. chinensis when compared with its allies, C. finetiana and C. armandii, which may be used as biomarkers. Thirteen proteins were up-regulated in C. finetiana (0.75-0.95 fold) and twelve proteins in C. armandii (1.05-1.66 fold) whilst seven proteins down-regulated (0.66-0.94 fold) in former and three proteins (1.07-1.20 fold) in later one in comparison with C. chinensis. Twenty five differential and similar protein spots were picked and analyzed by LC-MS/MS technique. Identified proteins are related to energy metabolism (ATP synthesis), photosynthesis. environmental stimuli, regulating RNA metabolism, growth hormone regulators, evolutionary trends and gene expression. The efficiency and applicability of proteomic approach as biomarker for identification of C. chinensis is discussed in its quality control (QC) perspectives. Leaf proteins of Clematis plants are explored for the first time by 2-DE technique and debated for their metabolic role. (author)

  10. Determination of taxonomic status of chinese species of the genus clematis by using high performance liquid chromatography-mass spectrometry (HPLC-MS) technique

    International Nuclear Information System (INIS)

    A comparative taxonomic study using chemometric and numerical taxonomic approaches on 15 populations of 12 species of major taxa of the genus Clematis, belonging to sections, Rectae, Clematis, Meclatis, Tubulosae and Viorna were analyzed by HPLC coupled with diode array detector and ESI-MS. The chemo diversity profile of saponins proved to be useful taxonomic markers for the genus and results are presented in pheno grams. The compound 'Huzhangoside D' was the most abundant in analyzed species of the genus. Numerical taxonomic study was also conducted based on phylogenetically informative characters which corroborated with chemical fingerprinting findings. The significance of chemical markers in taxonomic study as well as their correlation between morphology and chemical compound profile is also debated with its significant role in botanic drugs identification. (author)

  11. Changes in foliar nutrient content and resorption in Fraxinus excelsior L., Ulmus minor Mill. and Clematis vitalba L. after prevention of floods

    OpenAIRE

    Trémolières, Michèle; Schnitzler, Annik; Sanchez-Pérez, José-Miguel; Schmitt, Diane

    1999-01-01

    This paper focuses on the impact of flood on tree mineral nutrition through measurement of resorption (i.e. transfer of nutrients from leaves to perennial organs). Nutrient (N, P, K, Mg, Ca) concentrations in leaves of three representative species, Fraxinus excelsior L., Ulmus minor Mill. and Clematis vitalba L. were measured before and after abscission on flooded and unflooded hardwood forests of the upper Rhine plain. The nutrient concentrations in the soils, which were measured in the top ...

  12. Resource Investigation of Medicinal Species of Clematis in Yunnan Province%云南省铁线莲属药用资源调查

    Institute of Scientific and Technical Information of China (English)

    陈文允; 普春霞

    2006-01-01

    根据王文采院士的铁线莲属(Clematis)分类学研究结果,确定了云南铁线莲属的药用种类共计32种,7变种,并用野外调查结合昆明植物所馆藏标本确定其中15种具有一定的开发价值.

  13. Saponin-rich fraction from Clematis chinensis Osbeck roots protects rabbit chondrocytes against nitric oxide-induced apoptosis via preventing mitochondria impairment and caspase-3 activation

    OpenAIRE

    Wu, Wenjun; Gao, Xinghua; Xu, Xianxiang; Luo, Yubin; Liu, Mei; Xia, Yufeng; Dai, Yue

    2012-01-01

    Our previous study reported that the saponin-rich fraction from Clematis chinensis Osbeck roots (SFC) could effectively alleviate experimental osteoarthritis induced by monosodium iodoacetate in rats through protecting articular cartilage and inhibiting local inflammation. The present study was performed to investigate the preventive effects of SFC on articular chondrocyte, and explore the underlying mechanisms. Primary rabbit chondrocytes were cultured and exposed to sodium nitroprusside (SN...

  14. Researches into the Chemical Constituents of Clematis Huchouensis Tamura%湖州铁线莲化学成分研究

    Institute of Scientific and Technical Information of China (English)

    项佳佳; 谢琦婷; 张佳红; 葛海霞; 钱坤

    2014-01-01

    利用硅胶、ODS、Sephadex LH 20等色谱方法从湖州铁线莲Clematis huchouensis Tamura中分离得到9个化合物,根据理化性质和光谱数据,分别鉴定为正三十一烷醇(Ⅰ)、正二十五烷醇(Ⅱ)、正二十四烷酸(Ⅲ)、β谷甾醇葡萄糖苷(Ⅳ)、β豆甾醇葡萄糖苷(Ⅴ)、木犀草素(Ⅵ)、芹菜素(Ⅶ)、liriodendrin(Ⅷ)和芦丁(Ⅸ)。首次报道了湖州铁线莲植物中化学成分的研究,其中化合物Ⅰ和Ⅱ首次从铁线莲属植物中分离得到。%We have made researches into the chemical constituents of Clematis Huchouensis Tamura,and the findings show that the compounds are isolated by column chromatographic on silica gel,ODS and Sephadex LH 20.Their structures are identified on the basis of their physicochemical constants and spectral analysis.Nine compounds are isolated and elucidated as n hentriacontanol (Ⅰ), pentacosanol(Ⅱ),n tetracosanoic Acid(Ⅲ),β sitosterol β D glucoside(Ⅳ),stigmasterol 3 Oβ D glucopyranoside (Ⅴ),luteolin (Ⅵ),apigenin (Ⅶ),liriodendrin(Ⅷ)and rutin(Ⅸ).It is repor-ted the chemical constituents of Clematis Huchouensis Tamura for the first time,and compound ⅠandⅡ were isolated from Clematis genus for the first time.

  15. 川木通中4个化合物的研究%Studies on Four Compounds from Clematis Montana

    Institute of Scientific and Technical Information of China (English)

    杨泰然; 许枬; 石亚囡

    2008-01-01

    从毛茛科铁线莲属植物绣球藤(Clematis montana Buch.-Ham.)的乙醇提取物中分离得到4个化合物,并用理化常数和波谱解析的方法鉴定结构,分别确定为β-谷甾醇、阿魏酸、咖啡酸、Tachioside.

  16. Introduction Cultivation and Sexual Reproduction of Clematis heracleifolia%大叶铁线莲引种栽培与有性繁殖研究

    Institute of Scientific and Technical Information of China (English)

    潘杰; 王佳巍; 李滨胜

    2015-01-01

    Clematis heracleifolia,a kind of ground-covered plant,is introduced from Liaoning Province to Forest Bo-tanical Garden of Heilongjiang Province.Observation of botanical character,phenology,adaptability and sexual re-production for Clematis heracleifolia were conducted.Result shows that:(1 )Clematis heracleifolia can adapt to the soil and climate characteristics of Harbin area.The effect of landscape is optimal and should be promoted largly. (2)The optimum temperature of the seed germination is 18-21 ℃.Germination rate is the highest at 21 ℃,being 70%,and the germination potential are 58%.The germination rate of the seed are the highest in the very year.The vitality is the strongest.The germination rate and the vitality increase with the decrease of the storage years.%大叶铁线莲(Clematis heracleifolia )是黑龙江省森林植物园从辽宁省引种的地被植物。通过对其植物学特征、物候、适应性观测试验及有性繁殖试验。结果表明:(1)大叶铁线莲能适应哈尔滨的土壤和气候特点,景观效果良好,宜大量繁殖推广。(2)其种子最适宜发芽温度为18~21℃。在21℃时发芽率最高,为70%,发芽势为58%;当年的种子发芽率最高,生命力最强,种子的发芽率、生命力随储存年限增加而下降。

  17. 棉团铁线莲中新葡萄糖基萘类成分%A New Glucopyranosyl Naphthalene from Clematis hexapetala Pall

    Institute of Scientific and Technical Information of China (English)

    董彩霞; 史社坡; 赵明波; 屠鹏飞

    2008-01-01

    目的:研究棉团铁线莲(Clematis hexapetala Pall.)的干燥根及根茎的化学成分.方法:利用溶剂提取、硅胶柱色谱和高效液相色谱等技术进行分离、纯化,根据理化性质和波谱数据鉴定化合物的结构.结果:从中分离得到1个葡萄糖基萘类化合物,鉴定为5,8-二氢-6-甲基萘-1,4-二-O-β-D-葡萄糖苷 (1).结论:该化合物为一新化合物,丰富了棉团铁线莲的化学成分.%AIM: To study the chemical constituents of the roots and rhizomes of Clematis hexapetala Pall.. METHODS: The solvent extraction, silica gel column chromatography and pre-HPLC were used to isolate and purify the constituent, and various spectroscopic analyses were used to elucidate the structure. RESULTS: A glucopyranosyl naphthalene (1) derivative, 5,8-dihydro-6-methyl-1,4- β- D -diglucopyranosyl naphthalene has (1) been isolated from this plant. CONCLUSION: This compound is a new one, and this study enriched the chemical constituents of Clematis hexapetala.

  18. 铁线莲属黄花铁线莲组修订%A revision of Clematis sect. Meclatis (Ranunculaceae)

    Institute of Scientific and Technical Information of China (English)

    王文采

    2006-01-01

    Clematis sect. Meclatis is revised in this paper. Brief taxonomic history and geographical distribution of the section are given, its systematic position and the relationships among the species are discussed, and the evolutionary trends of some characters in the section are evaluated. Clematis akebioides (Maxim.) Veitch and C. tangutica (Maxim.) Korsh. are considered the primitive species in the section, whereas C. caudigera W. T. Wang and C. corniculata W. T. Wang are considered the advanced ones. The western edge of the Qinghai-Xizang (Tibet) plateau with the Pamirs and the adjacent mountains, the highest land mass in the world, where 10 species of the section are concentrated, is regarded as the distribution center, and the eastern edge of the Qinghai-Xizang (Tibet) plateau, where the two primitive species, C. akebioides and C. tangutica, sympatrically occur, may be the center of origin of the section. The inclusion of C. ispahanica Boiss. and C. graveolens Lindl. in sect. Meclatis by some authors is not accepted, with the former being a member of sect. Clematis, and the latter a member of sect. Brachiatae Snoeijer. A new variety, C. intricata Bunge var. intrapuberula W. T. Wang, is described, and two new combinations, C. tangutica var. mongolica (Grey-Wilson) W. T. Wang and C. tibetana Kuntze var. pamiralaica (Grey-Wilson) W. T. Wang, is proposed. As a result, 13 species and 13 varieties are recognized in sect. Meclatis. They are keyed, described, and illustrated.%对毛茛科铁线莲属Clematis的黄花铁线莲组sect. Meclatis进行了全面修订, 确定此属含13种和13变种(包括1新变种和2新变种等级);写出了此组的分类学简史和地理分布, 并对其在铁线莲属中的系统位置和组内诸种的亲缘关系进行了讨论;还写出了此组的分种、分变种检索表, 以及各种植物的形态描述、地理分布、生长环境等, 并附有各种的插图.此组的花构造与对枝铁线莲组sect. Brachiatae

  19. 河南铁线莲属植物分类学研究%Study on Species Classification of Clematis L. from Henan Province of China

    Institute of Scientific and Technical Information of China (English)

    闫双喜; 张志翔

    2010-01-01

    铁线莲属(Clematis L.)是一个在分类上较困难的类群,种类多,形态变化大.全球铁线莲属植物有350余种,中国有150余种.2006年7~8月我们对河南四大山区的铁线莲属植物种类进行了调查、采集和鉴定,标本存放于河南农业大学标本馆(HEAC),此外我们还认真观察了中国科学院植物研究所标本馆(PE)等国内各大标本馆的铁线莲属植物标本.结果表明河南铁线莲属植物24种和11变种,按照王文采等的铁线莲属新分类系统分别隶属于4亚属、7组、11亚组、15系,其中首次报道了半钟铁线莲(C. sibirica var.ochotensis)和长冬草(C. hexapetala var.tchefouensis)为河南铁线莲属植物新记录,首次报道了Clematis honanensis S.Y.Wang & C.L.Chang'作为Clematis pseudootophora M.Y.Fang in W.T.Wang的新异名.增补 (1981)中未记录的有5种5变种:女萎(C.apiifolia DC.)、毛萼铁线莲(C. hancockiana Maxim.)、大花威灵仙(C. courtoisii Hand.-Mazz.)、芹叶铁线莲(C. aethusifolia Turcz.)、巴山铁线莲(Clematis pashanensis(M.C.Chang)W.T.Wang)、扬子铁线莲(C.pubenda Hook.f.et Thorns.var.ganpiniana(Levl.et Vant.)W.T.Wang)、狭裂太行铁线莲(C. kirilowii Maxim.var.chanetii(Levl.)Hand.-Mazz.)、大花绣球藤(C.montana Buch.-Ham.ex DC.var.grandiflora Hook.)、毛叶威灵仙(C. chinensis Osbeck.var.vestita(Rhed.&Wils.)W.T.Wang)、狭卷萼铁线莲(Clematis tubulosa Turcz.vgr.ichangensis(Rehd.& Wils.)W.T.Wang).订正了(1981)中6个分类群的学名:将C. uncinata Champ.var.biternata W.T.Wang作为C. uncinata Champ.的异名,C. brevicaudata DC.var.filipes Rhed.作为C.puberula Hook.f.et Thoms vsr.tenuisepala(Maxim.)W.T.Wang的异名,C. brevicaudata DC.var.tenuisepala Maxim.作为C.puberula Hook.f.et Thoms.var.tenuisepala(Maxim.)W.T.Wang的异名,C. argentilucida(Levl.et Vant.)W.T.Wang作为C.grandidentata(Rehd.et Wils.)W.T.Wang的异名,C. obtusidentata(Rhed.et Wils.)H.Eichler作为C. apiifolia DC

  20. Morphological and functional study of the marginal sphincter of the sea anemones Phymactis clematis and Aulactinia marplatensis from intertidal of Mar del Plata, Argentina Estudio morfológico y funcional del esfínter marginal de las anémonas de mar Phymactis clematis y Aulactinia marplatensis del intermareal de Mar del Plata, Argentina

    Directory of Open Access Journals (Sweden)

    Elvira A. González Oliveira

    2009-09-01

    Full Text Available It was made the characterization of marginal sphincter to the species Phymactis clematis (Drayton in Dana, 1849 and Aulactinia marplatensis (Zamponi, 1977, from intertidal ecosystem through their morphogical and functional study. The species P. clematis has a circumscript sphincter of palmate type. This muscle is constituted by a mesogloeal axis and several mesogloeal subaxes. Axis as well as subaxes give a support to the endoderm which border is smooth. Aulactinia marplatensis has a circunscript sphincter pinnate type. The axis has a truncated cone shape while in P. clematis the shape is cylindrical on its origin and it is bifurcated at the end. Both species experiments were carried out using the isolated muscles. They were stimulated at increasing KCl concentrations ranging from 20 to 200 mM. The results were analysed in the form of dose-response curves expressed in tension in grams force vs concentration. Contractil force increases in a sigmoid form to increasing KCl concentrations. The correlation between morphology and function and the differences shown in both species would be related to their intertidal distribution.Se realizó la caracterización de las anémonas de mar Phymactis clematis (Drayton in Dana, 1849 y Aulactinia marplatensis (Zamponi, 1977 del ecosistema intermareal mediante estudio morfológico y funcional. La especie P. clematis tiene un esfínter circunscripto de tipo palmado. Este músculo está constituido por un eje mesogloeal y varios subejes mesogloeales. Tanto el eje como los subejes dan soporte al endodermo cuyo borde es liso. La especie A. marplatensis tiene un esfínter circunscripto de tipo pinnado. El eje tiene forma de cono truncado mientras que en P. clematis es cilíndrico en su origen bifurcándose en su parte final. Los experimentos fueron llevados a cabo usando el músculo aislado de ambas especies. Estos fueron estimulados a concentraciones crecientes de KCl en un rango de 20 a 200 mM. Los resultados

  1. 粗齿铁线莲扦插繁殖条件初探%Cutting Propagation of Clematis Grandidentata

    Institute of Scientific and Technical Information of China (English)

    杨璧嘉; 吴玉兰; 保智娟; 刘维君; 李宗艳; 唐岱; 王锦

    2014-01-01

    To provide theoretical basis for cuttage rooting of coarse teeth clematis(Clematis grandidentata﹚, L9(34﹚ orthogonal design test affecting the cutting was carried out. Results showed that rooting rate of all treatments was ranged from 36.67% to 83.30%. Rooting time needed 22 to 32 days. The root number was 12 to 23. The root length 39.4 to 87.7 cm. The indexes of treatment A 2B1C2 cutting slips were optimal when cutting matrix for peat soil:pastoral soil:perlite=3:1:1, naphthalene acetic acid (NAA﹚ concentration 400 mg/L, processing time 10 min, with 83.30% of rooting rate, 22.88 of average root number, 87.68 cm of average root length, and only 21.20 days of rooting time, 107.60μm/d of root growth rate, 91.30 μm/d of the leaf bud growth rate. The results of principal component analysis (PCA﹚ and subordinate function method showed that treatment A 2B1C2 was the best.%为给粗齿铁线莲(Clematis argentilucida)扦插繁殖提供理论依据,进行了影响扦插3个因子的L9(34)正交试验,3个因子分别为基质、生根剂浓度和生根剂处理时间。试验结果表明,粗齿铁线莲扦插生根率可达36.67%~83.30%;生根时间需22~32 d,根数12~23条,根长39.4~87.7 cm。A 2B1C2处理扦插基质为腐殖土:田园土:珍珠岩=3:1:1,外源激素萘乙酸(NAA)浓度为400 mg/L,处理时间10 min的插穗生根情况最优,插穗生根率达83.30%、平均根数22.88条、平均根长87.68 cm、生根所需时间21.20 d,根生长速率107.60μm/d,叶芽生长速率91.30μm/d。同时,主成分分析(PCA)以及隶属函数法分析结果也表明, A 2B1C2处理组合的插条生长情况最好。

  2. Conformational study reveals amino acid residues essential for hemagglutinating and anti-proliferative activities of Clematis montana lectin.

    Science.gov (United States)

    Lu, Bangmin; Zhang, Bin; Qi, Wei; Zhu, Yanan; Zhao, Yan; Zhou, Nan; Sun, Rong; Bao, Jinku; Wu, Chuanfang

    2014-11-01

    Clematis montana lectin (CML), a novel mannose-binding lectin purified from C. montana Buch.-Ham stem (Ranunculaceae), has been proved to have hemagglutinating activity in rabbit erythrocytes and apoptosis-inducing activity in tumor cells. However, the biochemical properties of CML have not revealed and its structural information still needs to be elucidated. In this study, it was found that CML possessed quite good thermostability and alkaline resistance, and its hemagglutinating activity was bivalent metal cation dependent. In addition, hemagglutination test and fluorescence spectroscopy proved that GuHCl, urea, and sodium dodecyl sulfate could change the conformation of CML and further caused the loss of hemagglutination activity. Moreover, the changes of fluorescence spectrum indicated that the tryptophan (Trp) microenvironment conversion might be related to the conformation and bioactivities of CML. In addition, it was also found that Trp residues, arginine (Arg) residues, and sulfhydryl were important for the hemagglutinating activity of CML, but only Trp was proved to be crucial for the CML conformation. Furthermore, the Trp, Arg, and sulfhydryl-modified CML exhibited 97.17%, 76.99%, and 49.64% loss of its anti-proliferative activity, respectively, which was consistent with the alterations of its hemagglutinating activity. Given these findings, Trp residues on the surface of CML are essential for the active center to form substrate-accessible conformation and suitable environment for carbohydrate binding. PMID:25239139

  3. 角蒿和铁线莲的矮化试验%Studies on stunting of Incarvillea and Clematis

    Institute of Scientific and Technical Information of China (English)

    李志坚; 管开云; 李景秀

    2003-01-01

    为了提高株型较高的毛子草(Incarvillea arguta)和半藤本性状的毛茛铁线莲(Clematis ranunculoides)的观赏价值,我们用多效唑、矮壮素和丁酰肼对它们进行了多种矮化处理.试验结果表明每盆用0.4 g 50%多效唑可湿性粉剂施于土壤,每两周全株喷0.25%的矮壮素并结合打顶可明显抑制第三年生毛子草的株高、花穗和羽状复叶的生长,增加了花穗数和花蕾总数,开花期比对照延长约10 d.每两周全株喷0.76%丁酰肼并结合打顶可抑制毛茛铁线莲的枝条生长,促进分枝,增加花蕾数.对两种植物的矮化均表明矮化处理过的植株对白粉病有较强的抗性.

  4. Chemical constituents of Clematis manshurica%东北铁线莲化学成分研究

    Institute of Scientific and Technical Information of China (English)

    史社坡; 蒋丹; 董彩霞; 屠鹏飞

    2007-01-01

    目的 研究东北铁线莲Clematis manshurica的化学成分.方法 利用硅胶、Sephadex LH-20、PHPLC等色谱方法进行化合物的分离纯化,根据化合物的理化性质、光谱数据进行结构鉴定.结果 分离得到11个化合物,分别鉴定为角鲨烯(Ⅰ)、木栓酮(Ⅱ)、正二十六烷酸(Ⅲ)、β-谷甾醇(Ⅳ)、豆甾醇(Ⅴ)、齐墩果酸(Ⅵ)、β-胡萝卜苷(Ⅶ)、5-羟甲基糠醛(Ⅷ)、丹参素甲酯(Ⅸ)、5R-5-羟甲基-2(3H)-呋喃酮(Ⅹ)、5R-5-羟甲基-2(5H)-呋喃酮(Ⅺ).结论 11个化合物除β-谷甾醇外均为首次从东北铁线莲中分离得到.

  5. Identifying a contact zone between two phylogeographic lineages of Clematis sibirica (Ranunculeae) in the Tianshan and Altai Mountains

    Institute of Scientific and Technical Information of China (English)

    Hong-Xiang ZHANG; Ming-Li ZHANG

    2012-01-01

    Clematis sibirica,a woody vine occurring primarily under conifer forests,is widespread in northern Eurasia.In this study,we intend to illustrate how the taxon has responded in the area of the Tianshan and Altai Mountains of Central Asia to the Pleistocene climatic fluctuations.The chloroplast intergenic spacer psbA-trnH was sequenced for 125 individuals from 28 populations,and a total of eight chlorotypes were identified.The presence of definite phylogeographic structure was detected for the species (NST > GST,P < 0.001),and phylogenetic analysis indicated that the eight chlorotypes were clustered into two divergent lineages.They split at approximately 550-690 ka BP,according to coalescence analysis,coincident with the Pleistocene maximum glacial stage in these mountains,which suggests the restriction of these lineages to separate refugia at that time.Spatial analysis of molecular variance likewise divided the sampled populations into two associations,an Altai and eastern Tianshan group (populations 1-17),and a western Tianshan group (populations 18-28).Low levels of genetic diversity and unimodal mismatch distributions were obtained for both of these groups,suggesting postglacial range expansions.During the course of these expansions,mountain ranges surrounding the Dzungarian Basin probably served as migration corridors.In addition,a contact zone was identified in the central Tianshan and eastern Altai Mountains between the two phylogeographic lineages.

  6. 黄花铁线莲新鲜全草化学成分研究%Chemical Constituents from Fresh Clematis intricata Bunge

    Institute of Scientific and Technical Information of China (English)

    杨晓军; 涂院海

    2011-01-01

    从黄花铁线莲(Clematis intricata Bunge)新鲜全草中分离得到7个化合物,经理化和光谱分析鉴定为正二十八烷醇(n-octacosanol) (1)、β-谷甾醇(2)、东莨菪素(3)、阿魏酸(4)、2-甲氨基苯甲酸(5)、水杨酸(6)和甘露醇(7).其中,化合物5,6和7首次从该属植物中分离得到.

  7. A new record subsection of the genus Clematis L. from Henan of China--Subsect. Acerifoliae W.T.Wang%河南铁线莲属一新记录亚组--槭叶铁线莲亚组

    Institute of Scientific and Technical Information of China (English)

    闫双喜; 何松林; 苏金乐; 曾效葵; 张凌

    2005-01-01

    报道了河南铁线莲属一新记录亚组--槭叶铁线莲亚组Subsect. Acerifoliae W.T.Wang,增补了(1981)未记录的1变种--无裂槭叶铁线莲Clematis acerifolia Maxim. Var. Elobata S. X. Yan.

  8. Determination of Oleanolic Acid in Roots and Stem-Leaf of Clematis hexapetala by HPLC%HPLC法测定棉团铁线莲中齐墩果酸的含量

    Institute of Scientific and Technical Information of China (English)

    关颖丽; 杨荣艳; 张海峰

    2011-01-01

    [目的]采用HPLC法测定棉团铁线莲(Clematis hexapetala)根及茎叶中齐墩果酸含量,为进一步开发棉团铁线莲药用资源提供理论依据.[方法]色谱柱为YW-C18柱(4.6 mm×150.0 mm,5 μm); 流动相为甲醇-水(V∶V=90∶10);检测波长为220 nm;进样量为10 μl;流速为1.0 ml/min;柱温为25 ℃.[结果]棉团铁线莲根中齐墩果酸的含量为0.175 2%,茎叶中齐墩果酸的含量为0.096 3%.[结论] 该方法简单、快速、准确,可用于棉团铁线莲中齐墩果酸的含量测定;同时该研究为评价棉团铁线莲茎叶的药用价值提供了参考,为进一步开发棉团铁线莲药用资源和质量控制提供了科学依据.%[ Objective ] To determine the content of oleanolic acid in roots and stem - leaf of Clematis hexapetala, so as to provide theoretical evidence for the further development of Clematis hexapetala resources. [ Method] HPLC conditions were as follows: YW-C18 column (4.6 mm × 150 mm ,5 μm) , mobile phase of methanol-water ( V∶ V = 90∶10 ), detection wavelength of 220 nm, sampling amount of 10 μl, flow rate of 1.0 ml/min and column temperature of 25 ℃. [ Result ] The content of oleanolic acid in roots and stem-leaf of Clematis hexapetala were 0.175 2% and was 0.096 3% respectively. [ Conclusion ] Simple and rapid in operations and accurate in determination, the method established in this study is favorable for the determination of oleanolic acid in Clematis hexapetala, This case provides reference for the assessment of the medicinal value of stems and leaves from Clematis hexapetala, and offers scientific basis for the further development and quality control of Clematis hexapetala resources.

  9. 铁线莲属铁线莲组修订%A revision of Clematis sect. Viticella (Ranunculaceae)

    Institute of Scientific and Technical Information of China (English)

    王文采

    2007-01-01

    (1)对毛莨科铁线莲属Clematis的铁线莲组sect.Viticella进行了分类学修订,确定此组包含13种,1亚种和2变种(包括2新种和1新变种等级),写出此组的分类学简史和地理分布;将此组划分为3亚组,4系,写出区分组下各级分类群的检索表,以及各种植物的形态描述,地理分布,生长环境等,并附有多幅插图.(2)特产我国东部的单型毛萼铁线莲亚组subsect.Hancockianae(花具4枚平展,不展宽的萼片,雄蕊无毛)被认为此组的原始群.铁线莲亚组subsect.Floridae(花具5-8枚平展,强烈展宽的萼片,雄蕊无毛,花粉具散孔)和湖州铁线莲亚组subsect.Viticellae(花具4枚渐升,多少展宽的萼片,雄蕊花丝常被缘毛,花粉具3沟)可能均由毛萼铁线莲亚组衍生而出.(3)在我国东部集中分布此组的3亚组,3系的8种,l亚种和1变种,这里是此组的分布中心,也可能是此组的起源中心.%(1) Clematis sect. Viticella is revised in this paper.Thirteen species,one subspecies,and two varieties,inclucling two new species, Clematis inciso-denticulata W. T. Wang and C.xiangguiensis W. T. Wang, and one new rank, C. cadmia lustrated, and classified into three subsections and four series. Brief taxonomic history and geographical distribution of the section are given. (2) The relationships among the infrasectional groups are briefly discussed. Subsect. Hancocokianae with 4-sepalled flowers, undilated spreading sepals, and glabrous stamens is considered the extant primitive group of sect. Viticella. Subsect. Floridae, characterized by having 5-8-sepalled flowers, spreading and strongly dilated sepals, glabrous stamens, and pantoporate pollen, and subsect. Viticellae, characterized by having 4-sepalled flowers, usually ascendingf more or less dilated sepals, usually ciliate stamen filaments, and tricolpate pollen, might all be derived from subsect. Hancockianae.(3) East China, where are concentrated eight species, one subspecies, and one variety

  10. A revision of Clematis sect. Tubulosae (Ranunculaceae)%铁线莲属大叶铁线莲组修订

    Institute of Scientific and Technical Information of China (English)

    王文采; 谢磊

    2007-01-01

    Clematis sect. Tubulosae is revised in this paper. Nine species, two varieties, and three forms are recognized and classified into two subsections. An identification key is provided, and each species is described and illustrated. Brief taxonomic history is given, along with a summary of pollen morphology and geographical distribution. The relationships among the infrasectional groups are also discussed: Subsect. Pinnatae, characterized by its scandent habit, bisexual flowers, white or pinkish, at length spreading, obovate-oblong sepals, and tricolpate pollen, is regarded as the more primitive group, whereas subsect. Tubulosae, characterized by its erect habit, usually polygamous flowers, blue or purple, erect, usually narrowly oblong sepals, and usually pantoporate pollen, is regarded as the more advanced group of the section. Subsect. Pinnatae is believed to have originated from sect. Clematis in Central or East China, and subsect. Tubulosae might be derived from subsect. Pinnatae.%对毛茛科Ranunculaceae铁线莲属Clematis的大叶铁线莲组sect.Tubulosae进行了修订,确定此组含9种2变种和3变型,对此组的分类简史和地理分布做了介绍,研究了此属大多数种的花粉形态,写出此组组下分类群的形态特征、地理分布等,并附有全部种的插图.此组9种被划分为2亚组,其中原始群羽叶铁线莲亚组subsect.Pinnatae(有2种,1种分布于中国河北和东北,另1种产日本)在木质藤本习性、花构造、花粉形态(具3沟)等方面与威灵仙组sect.Clematis颇为近似,区别主要在于萼片在开放初期近直立,以后平展,雄蕊花丝被毛,此亚组可能源于威灵仙组.进化群大叶铁线莲亚组subsect.Tubulosae(有7种,分布于我国东部、北部和台湾,朝鲜和日本)为直立多年生草本、小亚灌木或小灌木,花通常杂性,萼片直立(花萼呈筒状,稀呈坛状),顶端或上部反曲,雄蕊常有毛,花粉通常具散孔,只在1种(

  11. Adaptability of Eight Small-Flower Cultivated Varieties of Clematis L.%铁线莲8个小花品种的适生性比较

    Institute of Scientific and Technical Information of China (English)

    孟雪; 郝笑微; 王锦

    2011-01-01

    [目的]为筛选出适合昆明地区栽培的铁线莲(Clematis L.)品种,对从欧洲引进的8个小花品种的适生性进行研究.[方法]对8个小花品种(‘Hakuree’、‘Bluish violet’、‘Freda’、‘Markham's Pink’、‘Edis6900’、‘Eighteen Anna’、‘Japonica,、'Victoria,)主要进行萌芽期、现蕾期和初花期的观测及观赏价值的评价、抗病虫害能力等级的评定和适生性等级综合评定等研究.[结果]' Freda,、'Victoria,和‘Eighteen Anna’为适生型,能够正常开花,植株高度较高,攀爬效果较好,是较好的攀援植物,适宜在昆明推广应用;'Edis6900,可以定为较适生型,植株能够正常开花,花的直径较大,花朵的观赏效果较好,但攀援能力较差;‘Hakuree,、'Bluish violet,、'Markham's Pink,和‘Japonica’为不适生型,植株不能正常开花,表现出在昆明的不适生状态.[结论]该研究可为铁线莲属植物在昆明地区的推广应用提供参考,为铁线莲商品化生产提供栽培、管理提供理论依据.%[Objective] In order to select suitable cultivated varieties of Clematis L. In Kunming Area, adaptability of eight small-flower cultivated varieties of Clematis L. Were studied. [ Method] The germination period, budding stage, early flowering stage of eight small-flower cultivated varieties ( ' Hakuree' ,' Bluish violet' ,' Freda' ,' Markham ' s Pink',' Edis6900,' Eighteen Anna',' Japonica' ,' Victoria' ) were ovserved, and their ornamental value, ability of resistance to disease and pest and adaptability assessment were carried out. [ Result ]' Freda' ,' Victoria' ,' Eighteen Anna' can bloom normally,are better climbing plants that have higher height and better climbing effect in Kunming,so they are suitable types and are good for popularization and application in Kunming. ' Edis6900' can bloom normally, meanwhile its flowers have larger diameter and better viewing effect, but its climbing ability is bad in Kunming, so it

  12. 6种浙产铁线莲属植物的薄层色谱和紫外光谱组法鉴别%Identification of Six Plants of Clematis L.in Zhejiang Province by TLC and Ultraviolet Spectral Line Method

    Institute of Scientific and Technical Information of China (English)

    骆红飞; 高晓洁; 金艳

    2013-01-01

    目的:为威灵仙类药材的开发和利用提供鉴别依据.方法:采用薄层色谱法和紫外光谱组法对浙产6种铁线莲属植物进行分析鉴别.结果:扬子铁线莲、毛蕊铁线莲、柱果铁线莲的薄层色谱有其特征斑点;6种铁线莲属植物的紫外谱线组图谱存在差异.结论:方法简便、可靠,可用于鉴别该6种铁线莲属植物.%Objective: To provide a basis for the identification for developing and utilizing Medicinal Materias of Weil-ingxian. Method:The chemical constituents of six plants of Clematis L. in Zhejiang province were analysed by TLC and Ultraviolet line method. Result: Clematis ganpiniana, Clematis lasiandra Maxim. and Clematis uncinata had their unique spots in the TLC; the UV spectrum of six plants of Clematis L. were different. Conclusion: The method was smiple and stable , and can be used to identify these six plants of Clematis L.

  13. 黄花铁线莲根部的化学成分%Chemical constituents from the root of Clematis intricata Bunge

    Institute of Scientific and Technical Information of China (English)

    杨晓军; 涂院海

    2011-01-01

    从黄花铁线莲Clematis intricata Bunge根部分离得到6个化合物,分别鉴定为:7β-乙氧基-6α-羟基-1,4-二氧杂环辛烷(1),4,6,7-三甲氧基-5-甲基香豆素(2),东莨菪素(3),3,5,7,4'-四羟基黄酮(4),对羟基苯甲酸(5),3,4-二羟基苯甲酸(6).其中化合物1为新化合物,化合物2、5、6首次从该属植物中分离得到.

  14. 铁线莲属翅果铁线莲组修订%A revision of Clematis sect.Pterocarpa (Ranunculaceae)

    Institute of Scientific and Technical Information of China (English)

    王文采

    2006-01-01

    对毛茛科铁线莲属Clematis中单型的翅果铁线莲组sect.Pterocarpa进行了分类学修订,写出了此组及其惟一种,翅果铁线莲C.brachyura的分类学简史和形态描述,给出此种的插图.根据其体态及花构造近似亚洲东部的辣蓼铁线莲C.mandshurica Rupr.和圆锥铁线莲C.terniflora DC.(二种均为威灵仙组sect.Clematis的成员),推测翅果铁线莲可能源自威灵仙组.%Clematis sect.Pterocarpa is revised in this paper. Brief taxonomic history,description,and illustration of the monotypic section with its only species,C.brachyura,are given,and the origin of it is briefly discussed.

  15. 黄花铁线莲乙酸乙酯组分的抗炎作用研究%Study on Anti-inflammatory Effect of Ethylacetate Extract from Clematis intricata Bunge

    Institute of Scientific and Technical Information of China (English)

    杨晓军; 高芬; 涂院海; 宋延平

    2012-01-01

    [目的]研究黄花铁线莲乙酸乙酯组分的抗炎作用.[方法]将试验动物随机分为4组,每组10只,分别为模型对照组、复方七叶皂苷钠凝胶组和黄花铁线莲高、低剂量组(0.08和0.04 g/kg),连续外涂给药7d,采用二甲苯诱发小鼠耳肿胀法和交叉菜胶诱发大鼠足跖肿胀观察抗炎作用.[结果]黄花铁线莲乙酸乙酯组分可以明显抑制二甲苯诱发小鼠耳肿胀和交叉菜胶诱发大鼠足跖肿胀.[结论]黄花铁线莲乙酸乙酯组分具有良好的抗炎活性.%[Objective] To study the anti-inflammatory effect of ethylacetate extract from Clematis intricata Bunge. [Method] The animals were randomly divided into four groups, ten each group, including the control group, compound sodium aescinate gel group, low and high dose of Clematis intricata Bunge group(at doses of 0.08 and 0.04 g/kg accordingly) for 7 days. Model of ear swelling in mice and model of paw edema in rats were adopted to observe the anti-inflammatory effect. [Result] The ethylacetate extract from Clematis intricata Bunge could significantly decrease the raw edema and inhibit the ear edema. [Conclusion] Ethylacetate extract from Clematis intricata Bunge has obvious anti-inflamma-tion.

  16. 河南槭叶铁线莲(毛茛科)一新变种--无裂槭叶铁线莲%Clematis acerifolia var. elobata, a new variety of the Ranunculaceae from Henan, China

    Institute of Scientific and Technical Information of China (English)

    闫双喜; 王鹏飞; 朱长山; 杨秋生; 孔德政

    2005-01-01

    报道了槭叶铁线莲一新变种--无裂槭叶铁线莲Clematis acerifolia Maxim. var. elobata S. X. Yan.该变种与原变种不同在于植株较矮,高不超过20 cm,叶卵形至宽卵形,不分裂,基部宽楔形至近截形,边缘具不规则锯齿.

  17. Experimental Study of Anti-inflammatory Effect of Extraction of Clematis heracleifolia%大叶铁线莲提取物抗炎作用实验研究

    Institute of Scientific and Technical Information of China (English)

    邢天天; 李春磊; 王义坤; 郭秋霜

    2014-01-01

    目的:探讨大叶铁线莲粗提物的抗炎作用。方法:分别采用蛋清诱导大鼠足跖肿胀法、二甲苯致小鼠耳肿胀法研究大叶铁线莲提取物的抗炎作用。结果:大叶铁线莲提取物对大鼠足跖肿胀具有明显的抑制作用(醇提物P<0.01,水提物P<0.05),显著减轻二甲苯致小鼠耳肿胀程度(P<0.05)。结论:大叶铁线莲水提物、醇提物具有明显的抗炎作用。%Objective:To explore the anti-inflammatory effect of extraction of Clematis heracleifolia. Methods:To study the effect of anti-inflammatory of water exteraction and alcoholic extraction of Clematis heracleifolia through inflammatory rat models with swollen foot induced by egg white and mouse models with swollen ears induced by xylol respectively. Results:Clematis heracleifolia crude extraction has an obvious inhibitory effect on rat paw swelling(alcoholic extraction P<0.01,water extraction and P<0.05) and can retrain ear tumefaction of mice (P<0.05). Conclusion:The water extraction and alcoholic extraction of Clematis hera-cleifolia have significant anti-inflammatory effects.

  18. 中药威灵仙的化学成分及药理作用研究进展%Research Progress On Chemical Constituents and harmacological Effects of Traditional Chinese Medicine Clematis Chinensis

    Institute of Scientific and Technical Information of China (English)

    李佳; 刘继永

    2011-01-01

    对已报道的关于中药威复仙化学成分及药理作用的文献进行综述,为进一步开展深入研究及在临床上应用提供资料参考.%Studies about the chemical constituents and pharmacological effects of traditional Chinese medicine Clematis chinensis (wei ling xian ) were summarized in this paper, to provide references for further research and clinical practice.

  19. 分光光度法测定威灵仙中总皂苷的含量%Content of Saponin in Chinese Clematis Root Detected by Absorption Spectrometry

    Institute of Scientific and Technical Information of China (English)

    万德光; 裴瑾; 唐远; 范刚

    2007-01-01

    目的:测定威灵仙药材中总皂苷含量,建立威灵仙药材质量控制方法.方法:以三萜皂苷元齐墩果酸为对照,采用分光光度法.结果:齐墩果酸在0.0024,0.0480 mg/mL内线形关系良好(r=0.9998),平均回收率为95.51%,RSD=1.01%,测得威灵仙Clematis chinensis 中总皂苷含量为12.71%,东北铁线莲Clematis Terniflora var.mandshurica(Rupr)Ohwi.中总皂苷含量为9.21%,棉团铁线莲Clematis hexapetala Pall.中总皂苷含量为17.06%,其伪品菊科植物显脉旋复花Inula nervosa Wall.中总皂苷含量为4.95%,同属混淆品植物锥花铁线莲Clematis Panjculata Thunb.中总皂苷含量为2.24%.结论:本方法简便、准确、重现性好,可以将总皂苷含量做为威灵仙药材的质量控制指标,且表明正品威灵仙药材中总皂苷含量均比其伪品和混淆品的高.

  20. 威灵仙多糖对舌鳞癌细胞生长抑制作用的研究%Polysaccharide from Clematis chinensis Osbeck Inhibited the Growth of Tongue Squamous Cell Carcinoma

    Institute of Scientific and Technical Information of China (English)

    李俊妍; 钟辉; 李德超

    2011-01-01

    Objective: To study the inhibitory effect of polysaccharides from Clematis chinensis on the human tongue squamous cell carcinoma Tca-8113 cells.Methods: Clematis chinensis polysaccharides were extracted, isolated and purified.The human tongue squamous cell cancer Tca-8113 cells were cultured in vitro with six extracts at different concentrations and incubation times.The growth of cancer cells was analyzed by MTT method.Results:Clematis chinensis polysaccharides significantly inhibited the growth of Tca-8113 cells in a dose dependent mannmer.Conclusion: The polysaccharides from Clematis chinensis showed growth inhibition effect on human tongue squamous cell carcinoma Tca-8113 cells, indicating its potential role in human tongue cancer therapy.%目的:探讨威灵仙多糖对人舌鳞癌细胞Tca-8113的体外生长抑制作用.方法:提取分离纯化威灵仙多糖,用噻唑蓝(MTT)法测定在不同浓度威灵仙多糖作用下人舌鳞癌细胞Tca-8113的活力,观察药物对癌细胞的生长抑制作用.结果:威灵仙多糖对Tca-8113的生长具有明显的抑制作用,随着威灵仙多糖浓度的增大或作用时间延长,抑制作用逐渐增强,呈一定的剂量和时间依赖关系.结论:在体外实验中,威灵仙多糖对人舌鳞癌细胞Tca-8113具有明显的杀伤和抑制作用,可进一步研究其作为抗肿瘤药物应用于临床的潜力.

  1. Comparison on Four Extraction Methods of Genomic DNA from Clematis fasciculiflora Franch%4种滑叶铁线莲基因组DNA提取方法比较

    Institute of Scientific and Technical Information of China (English)

    胡祎晨; 孙正海; 王锦; 李世峰; 辛培尧; 范萱

    2011-01-01

    [Objective ] This study aimed at comparing the four extraction methods of genomic UNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch. [ Method] Leavies of Clematis fas-cicidiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ , improved CTAB method Ⅱ , improved CTAB method Ⅲ and improved SDS method. [Result] The four extraction methods could all successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch. The purity of genomic DNA was the highest using improved CTAB method I , with the longest extracting time; while the concentration of genomic DNA was the maximum using the improved SDS method, with the shortest extracting time and relatively low purity; the extracting time of improved CTAB method Ⅲ was the shortest. [ Conclusion ] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.%[目的]对4种滑叶铁线莲基因组DNA提取方法进行比较研究,建立滑叶铁线莲最适DNA提取方法.[方法]以滑叶铁线莲叶片为材料,比较改良CTAB法Ⅰ、改良CTAB法Ⅱ、改良CTAB法Ⅲ、改良SDS法这4种基因组DNA提取法在提取DNA纯度、浓度和提取时间等方面的不同.[结果]4种方法都可提取滑叶铁线莲基因组DNA.改良CTAB法Ⅰ提取DNA纯度最高,但浓度最低且提取时间最长;改良SDS法提取DNA浓度最高,所需时间较短,但纯度较低;改良CTAB法Ⅲ提取所需时间最短.[结论]建立了铁线莲最适DNA提取方法,为运用分子生物学手段对其研究提供支持.

  2. Study on extraction technology of total polyphenols in Clematis Manshurica Rupr%东北铁线莲总多酚的超声提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    王晓林; 钟方丽; 孙威; 王慧竹

    2012-01-01

    探索超声波辅助提取东北铁线莲总多酚的最佳工艺条件.以东北铁线莲总多酚的提取率作为评价指标,选择乙醇体积分数、提取时间、料液比、提取次数为考察因素,采用正交试验法确定了东北铁线莲中总多酚的最佳工艺,采用分光光度法对提取液中总多酚的含量进行了测定,检测波长为766 nm.结果表明:最佳提取工艺条件中乙醇浓度为55%(体积分数),提取时间为35 min,料液比(质量比)为1∶30,提取次数为3次.没食子酸在质量浓度为1.116~11.160 μg/mL时呈现良好的线性关系.该提取方法操作简单,结果比较可靠,适用于东北铁线莲总多酚的提取.%This paper is to establish the optimum extraction technology of total polyphenols (TP) in Clematis manshurica Rupr. By ultrasonic extraction method. By using the extraction efficiency of TP in Clematis manshurica Rupr. As evaluating indicator, some important factors, such as the concentration of ethanol, the extracting time, the ratio between raw material and solvent and the extracting times, are optimized by orthogonal experimental design. The content of TP in Clematis manshurica Rupr. Is determined by spectrum photometric method at detection wavelength of 766 nm. The optimum extraction condition of TP in Clematis manshurica Rupr. With ultrasonic method are obtained as follows: the concentration of ethanol, the extracting time is 35 min, the ratio of raw material to solvent is 1 ! 30, and the extraction times is 3 times is 55%. The concentration of gallic acid and its absorption value shows a good linear relationship at the range of 1. 116 ~11. 160μg/mL. It is concluded that the ultrasonic extraction method obtained in the paper is easy to operate and reliable, so it could be used for extracting TP in Clematis manshurica Rupr.

  3. Comparison on Four Extraction Methods of Genomic DNA from Clematis fasciculiflora Franch%4种滑叶铁线莲基因组DNA提取方法比较

    Institute of Scientific and Technical Information of China (English)

    胡祎晨; 孙正海; 王锦; 李世峰; 辛培尧; 范萱

    2011-01-01

    [目的]对4种滑叶铁线莲基因组DNA提取方法进行比较研究,建立滑叶铁线莲最适的DNA提取方法。[方法]以滑叶铁线莲叶片为材料,比较改良CTAB法Ⅰ、改良CTAB法Ⅱ、改良CTAB法Ⅲ、改良SDS法这4种基因组DNA提取法在提取的DNA纯度、浓度和提取时间等方面的不同。[结果]4种方法都可提取滑叶铁线莲基因组DNA。改良CTAB法Ⅰ提取DNA纯度最高,但浓度最低且提取时间最长;改良SDS法提取DNA浓度最高,所需时间较短,但纯度较低;改良CTAB法Ⅲ提取所需时间最短。[结论]建立了铁线莲最适DNA提取方法,为运用分子生物学手段对其研究提供支持。%[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch.[Method] Leavies of Clematis fasciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ,improved CTAB method Ⅱ,improved CTAB method Ⅲ and improved SDS method.[Result] The four extraction methods could all be successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch.The purity of genomic DNA was the highest using improved CTAB method Ⅰ,with the longest extracting time;while the concentration of genomic DNA was the maximum using the improved SDS method,with the shortest extracting time and relatively low purity;the extracting time of improved CTAB method Ⅲ was the shortest.[Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.

  4. 棉团铁线莲总皂甘抗肿瘤作用研究%Antitumor Activities of Total Saponins from Clematis Hexapetala Pall

    Institute of Scientific and Technical Information of China (English)

    杨林; 姚广玉; 薛鸿燕; 杨明俊

    2011-01-01

    Objective:To investigate the antitumor effect of total saponins of Clematis hexapetala Pall.Methods :The total saponins from Clematis hexapetala Pall.were prepared by ethanol extraction and macroporous resin.Its anti - tumor activity in vitro and in vivo was evaluated by MTT colorimetric assay and H22 cells - bearing mice models.Results:The results showed that the total saponins of Clematis hexapetala Pall.significantly inhibited proliferation of the human hepatoma cell lines HepG2, SMMC -7721 and the human cervical carcinoma cell Hela at the concentration of 0.1 -1mg·mL-1.The IC50 value on these three tumor cells were 0.5298mg·mL-1 ,0.2047mg·mL-1 and 0.2877mg·mL-1 respectively.In vivo, high ( 1.5 g· kg - 1 ), middle ( 0.75 g· kg - 1 ) and low (0.375 g· kg - 1 ) dose total saponins of Clematis hexapetala Pall.also significantly suppressed the tumor growth with tumor inhibition rate of 41.55% ,34.96% and 32.37%respectively.The slice through the pathological observation of tumor tissue also indicated that it has good antitumor activity.However, it can also reduce the spleen index and displays certain inhibitory action to the immune organ of tumor -bearing mice.Conclusion:The total saponins from Clematis hexapetala Pall.has a significant antitumor effect, but it also can suppress immune function of experimentals animals in vivo.%目的:研究棉团铁线莲总皂苷的体内外抗肿瘤作用.方法:采用乙醇提取、大孔树脂除糖的方法制备棉团铁线莲总皂苷,以MTT法和移植性H22实体荷瘤小鼠为模型,检测不同剂量棉团铁线莲总皂苷的体内外杭肿瘤作用.结果:当质量浓度为0.1-lmg·mL-1时,其对体外培养的3株人肿瘤细胞HepG2、SMMC-7721和Hela的增值表现出显著的抑制作用,IC50依次为0.5298mg·mL-1、0.2047mg·mL-1、0.2877mg·mL-1.体内实验表明,棉团铁线莲总皂苷高(1.5g·kg-1)、中(0.75g·kg-1)、低(0.375g·kg-1) 3剂量组也较好的抑制了荷瘤小鼠瘤块

  5. 畲药铁线莲提取物的生物活性初步研究%Studies on BioIogicaI Activities of Crude PIant Extracts from Clematis Hyb-ridas

    Institute of Scientific and Technical Information of China (English)

    叶丽颖; 腾春福; 吴楠; 朱扶蓉

    2015-01-01

    目的:研究铁线莲的生物活性,寻找药理活性成分。方法通过测定铁线莲石油醚提取物、乙醇提取物和水提取物的总还原力、对超氧阴离子( O2-)的清除率、对脂质体过氧化的抑制率来探讨铁线莲提取物的抗氧化作用;通过测定提取物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的抑制作用探讨铁线莲提取物的抑菌作用。结果铁线莲提取物对超氧阴离子有一定的清除能力,对脂质体过氧化有较强的抑制能力;乙醇提取物对金黄色葡萄球菌、大肠埃希氏菌和枯草芽孢杆菌有较好的抑菌效果,最低抑菌浓度分别为1、0.25、1mg・ mL-1;水提取物对大肠埃希氏菌和枯草芽孢杆菌也有较好的抑菌效果,最低抑菌浓度分别为1、1 mg・ mL-1。结论铁线莲提取物具有一定的抗氧化能力和抑菌能力。%OBJECTIVE To study the biological activities and the activite constituents of clematis hybrid-as.METHODS The total antioxidant capacity of clematis hybridas petroleum ether extract of,ethanol extract and water extract was determined by Optimized Ferricyanide/Prussian Blue Method,the superoxide anion radical scaven-ging by ways of pyrogallol oxidation systems,lipid peroxidation by means of 2-thiobarbituric acid( TBA);The bacteri-ostatic circle diameter size of clematis hybridas extract was determined by filter method,the minimal inhibitory con-centrations ( MIC) of clematis hybridas extract was determined by the growth rate method.RESULTS The results showed that the clematis hybridas extract have an effective antioxidant capacity as well as the power of reducing the activity of free radicals O2-and the capable of inhibiting lipid peroxidation,and have a good dose-effect relationship between the antioxidant activity and its concentration;The clematis hybridas alcohol extract can inhibit staphylococcus aureus,bacillus subtilis,e.coli,.the minimal inhibitory concentration

  6. Study on the Chemical constituents of a Traditional Mogolian Medicine HERBA CLEMATIS%蒙药材细叶铁线莲化学成分研究*

    Institute of Scientific and Technical Information of China (English)

    包保全; 乌雅罕; 陈建平; 布仁; 包娜; 张屏

    2015-01-01

    This study was aimed to isolate and characterize of chemical constituents in a traditional Mongolian medicine HERBA CLEMATIS. Normal and reverse phase coloumn chromatography, gel filtration chromatography sephadex LH-20, and preparative HPLC were used for isolation and purification compounds from the water extraction of the arial part ofC. aethusaefoliaTurcz. The planar structures and spatial configurations of isolated compounds were identified by high resolution MS, 1D and 2D NMR, and other spectrographic methods. The chemical research on the Mongolian medicine results 6 compounds, dihydrodehydrodiconiferyl alcohol (1), syringaresinol (2), pinoresinol (3), epi-pinoresinol (4), lirioresinol B dimethyl ether (5) and loliolide (6). All the compounds were isolated from this plant for the first time.%目的:研究蒙药材细叶铁线莲(HERBA CLEMATIS)中主要化学成分。方法:利用正、反相柱色谱,凝胶色谱LH20和制备型HPLC等方法,对芹叶铁线莲(Clematis aethusaefoliaTurcz.)干燥地上部分的水提取物进行分离纯化;得到的单体化合物经过高分辨质谱(HRMS)、核磁共振(1D、2D-NMR)等光谱方法,鉴定其平面结构和立体构型。结果:从细叶铁线莲水提取物中分离得到6个化合物,分别鉴定为二氢去氢二聚松柏醇(1),丁香脂素(2),松脂素(3),表松脂素(4),里立脂素B二甲醚(5)和黑麦草内酯(6)。结论:所有化合物均为首次从该植物中分离得到。

  7. Hederagenin, a major component of Clematis mandshurica Ruprecht root, attenuates inflammatory responses in RAW 264.7 cells and in mice.

    Science.gov (United States)

    Lee, Chul Won; Park, Sang Mi; Zhao, Rongjie; Lee, Chu; Chun, Wonjoo; Son, Yonghae; Kim, Sung Hun; Jung, Ji Yun; Jegal, Kyung Hwan; Cho, Il Je; Ku, Sae Kwang; Kim, Young Woo; Ju, Seong A; Kim, Sang Chan; An, Won G

    2015-12-01

    Clematis mandshurica Ruprecht root has been used in Asia as a traditional anti-inflammatory, analgesic, and antitumor agent. Its main active component is hederagenin, a naturally occurring triterpene, and in this study, we examined the anti-inflammatory effects of hederagenin in lipopolysaccharide-stimulated RAW 264.7 cells using an enzyme-linked immunosorbent assay, Western blot, and RT-PCR. In addition, its effects on acute inflammation in vivo were observed using a carrageenan-induced mouse hind paw edema assay. Furthermore, the changes on the histopathology and histomorphometry of hind paw skins were examined using carrageenan-treated mice. Treatment with hederagenin (10, 30 and 100μM) resulted in inhibited levels of protein expression of lipopolysaccharide-stimulated iNOS, COX-2, and NF-κB as well as production of NO, PGE2, TNF-α, IL-1β, and IL-6 induced by lipopolysaccharide. Consistent with these results, hederagenin also dose-dependently reduced the lipopolysaccharide-induced mRNA levels of iNOS and COX-2, and of the above-mentioned cytokines. Interestingly, results of the carrageenan-induced mouse hind paw edema assay showed an anti-edema effect of hederagenin. Furthermore, hederagenin (30mg/kg) inhibited the carrageenan-induced increases in skin thicknesses, infiltrated inflammatory cells, and mast cell degranulation. These results suggest that hederagenin may possess anti-inflammatory activities. PMID:26481049

  8. Saponin-rich fraction from Clematis chinensis Osbeck roots protects rabbit chondrocytes against nitric oxide-induced apoptosis via preventing mitochondria impairment and caspase-3 activation.

    Science.gov (United States)

    Wu, Wenjun; Gao, Xinghua; Xu, Xianxiang; Luo, Yubin; Liu, Mei; Xia, Yufeng; Dai, Yue

    2013-03-01

    Our previous study reported that the saponin-rich fraction from Clematis chinensis Osbeck roots (SFC) could effectively alleviate experimental osteoarthritis induced by monosodium iodoacetate in rats through protecting articular cartilage and inhibiting local inflammation. The present study was performed to investigate the preventive effects of SFC on articular chondrocyte, and explore the underlying mechanisms. Primary rabbit chondrocytes were cultured and exposed to sodium nitroprusside (SNP), a NO donor. After treatment with different concentrations of SFC (30, 100, 300, 1,000 μg/ml) for 24 h, nucleic morphology, apoptotic rate, mitochondrial function and caspase-3 activity of chondrocytes were examined. The results showed that SNP induced remarkable apoptosis of rabbit chondrocytes evidenced by Hoechst 33258 staining and flow cytometry analysis, and SFC prevented the apoptosis in a concentration-dependent manner. Further studies indicated that SFC could prevent the depolarization of mitochondrial membrane potential (∆ψm) in SNP-treated chondrocytes and suppress the activation of caspase-3. It can be concluded that the protection of SFC on articular chondrocytes is associated with the anti-apoptosis effects via inhibiting the mitochondrion impairment and caspase-3 activation. PMID:22821055

  9. Proteomic and Metabolomic Analyses of Leaf from Clematis terniflora DC. Exposed to High-Level Ultraviolet-B Irradiation with Dark Treatment.

    Science.gov (United States)

    Yang, Bingxian; Wang, Xin; Gao, Cuixia; Chen, Meng; Guan, Qijie; Tian, Jingkui; Komatsu, Setsuko

    2016-08-01

    Clematis terniflora DC. has potential pharmaceutical value; on the contrary, high-level UV-B irradiation with dark treatment led to the accumulation of secondary metabolites. Metabolomic and proteomic analyses of leaf of C. terniflora were performed to investigate the systematic response mechanisms to high-level UV-B irradiation with dark treatment. Metabolites related to carbohydrates, fatty acids, and amino acids and/or proteins related to stress, cell wall, and amino acid metabolism were gradually increased in response to high-level UV-B irradiation with dark treatment. On the basis of cluster analysis and mapping of proteins related to amino acid metabolism, the abundances of S-adenosylmethionine synthetase and cysteine synthase as well as 1,1-diphenyl-2-picrylhydrazyl scavenging activity were gradually increased in response to high-level UV-B irradiation with dark treatment. Furthermore, the abundance of dihydrolipoyl dehydrogenase/glutamate dehydrogenase and the content of γ-aminobutyric acid were also increased following high-level UV-B irradiation with dark treatment. Taken together, these results suggest that high-level UV-B irradiation with dark treatment induces the activation of reactive oxygen species scavenging system and γ-aminobutyric acid shunt pathway in leaf of C. terniflora. PMID:27323210

  10. Clematis hagiangensis N. T. Do, a new species of Ranunculaceae from Vietnam%越南铁线莲属一新种

    Institute of Scientific and Technical Information of China (English)

    Nguyen Thi DO

    2006-01-01

    本文描述的产于越南的毛茛科Ranunculaceae铁线莲属Clematis一新种C. hagiangensis N. T. Do是欧亚大陆第一个具单性花的种, 在花构造方面与单性铁线莲组单性铁线莲亚组sect. Aspidanthera Spach subsect. Dioicae (Prantl) W. T. Wang的种类近缘, 但叶均为单叶, 萼片呈卵形或宽卵形而不同.在单性铁线莲亚组的种, 叶通常为复叶, 只在C. dimorphophylla W. T. Wang和C. variifolia W. T. Wang同时为单叶和复叶, 此外萼片呈长圆形、倒披针形或狭卵形.%Abstract Clematis hagiangensis N. T. Do, a new species of the Ranunculaceae, is described from Vietnam. In floral structure this new species is related to the American species of sect. Aspidanthera subsect. Dioicae, but differs in its simple leaves and ovate or broadly ovate sepals. This section is newly recorded in the Eurasian continent.

  11. Studies on chemical constituents from root of Clematis hexapetala%棉团铁线莲化学成分研究Ⅰ

    Institute of Scientific and Technical Information of China (English)

    董彩霞; 史社坡; 武可泗; 屠鹏飞

    2006-01-01

    目的:研究棉团铁线莲Clematis hexapetala的干燥根及根茎的化学成分.方法:利用溶剂提取,硅胶柱色谱和高效液相色谱等手段进行分离、纯化,根据理化性质和波谱数据进行结构鉴定.结果:分离鉴定了9个化合物,分别为:木栓酮(1),白头翁素(2),β-谷甾醇(3),棕榈酸(4),香草酸(5),异落叶松脂素(6),5-羟甲基-2-呋喃酮(7),正壬烷(8),胡萝卜苷(9).结论:均为首次从该植物中分离得到.

  12. Therapeutic effect of the saponin fraction from Clematis chinensis Osbeck roots on osteoarthritis induced by monosodium iodoacetate through protecting articular cartilage.

    Science.gov (United States)

    Wu, Wenjun; Xu, Xianxiang; Dai, Yue; Xia, Lunzhu

    2010-04-01

    The objective of the present study was to investigate the effect of the saponin fraction from Clematis chinensis Osbeck roots (SFC) on an osteoarthritis model in rats and to explore its underlying mechanisms. Osteoarthritis was induced by intraarticular injection of monosodium iodoacetate (MIA) into knee joints of rats, and SFC and diclofenac were orally administered once a day for 28 consecutive days. Joint swelling, macroscopic observation, histological assessment and proteoglycan (PG) degradation were examined. In vitro, cultured rabbit chondrocytes were stimulated with MIA and sodium nitroprusside (SNP), respectively. The effects of SFC on MIA- and SNP-induced chondrocyte injury were examined by MTT assay. It was shown that SFC (50, 100, 200 mg/kg) dose-dependently reduced cartilage injury and PG degradation induced by MIA. Diclofenac (4 mg/kg) only slightly alleviated cartilage injury and PG degradation. SFC also prevented SNP- or MIA-induced rabbit chondrocyte impairment. These results indicate that SFC is effective in ameliorating joint destruction and cartilage erosion in MIA-induced osteoarthritic in rats, and the mechanisms of action for protecting articular cartilage are through preventing extracellular matrix degradation and chondrocyte injury. PMID:19655297

  13. Effects of Different Water Temperature on Seeds Germination of Clematis lasiandra Maxim%不同水温浸种对毛蕊铁线莲种子发芽指标的影响

    Institute of Scientific and Technical Information of China (English)

    黎彩霞; 廖明安; 魏志强; 沈文; 任雅君

    2011-01-01

    [Objective]The aim was to discuss on the effects of different water temperature on the seed germination indexes of Clematis lasiandra Maxim,in order to supply theoretical and practical basis for its reproduction. [Method]seeds were soaked in constant-temperature incubator under 10,15,20,25,35,and 45 ℃. Germination rate,germination index,germination energy,vigor index,of Clematis lasiandra Maxim were determined.[ Result] The results showed that the appropriate temperature for seeds of Clematis lasiandra Maxim was 20 ℃. The germination rate was 80.7% ,germination index was 6.184,germination energy was 54.0% ,and the vigor index was 9.442. [ Conclusion] After soaking in water under 20 ℃,the indices of Clematis lasiandra Maxim germination were the highest.%[目的]探讨不同水温浸种对毛蕊铁线莲种子发芽指标的影响,为毛蕊铁线莲种子繁殖提供理论和实践依据.[方法]利用恒温培养箱,经10和15℃(室温条件下的自来水温度,以此作为对照,CK),20、25、35和45℃的恒温水浸种48h后,测定毛蕊铁线莲种子发芽率、发芽指数、发芽势及活力指数5个指标.[结果]毛蕊铁线莲种子水温浸种的适宜温度为20C,其发芽率为80.7%,发芽指数为6.184,发芽势为54.0%,活力指数为9.442.[结论]20℃水温浸种后毛蕊铁线莲种子的各项发芽指标最高.

  14. Clematis属植物とその関連生薬の研究(第8報): Clematis uncinataおよび近縁種の地上部に由来する「威霊仙」

    OpenAIRE

    御影, 雅幸; 中島, 由仁; 難波, 恒雄

    1990-01-01

    The Chinese crude drug "Wei ling xian" (威霊仙) is derived from the underground portions of Clematis species. However, the aerial parts of the plants are also sold under the same name in Guangxi (広西壮族自治区) and Sichuan (四川省) Provinces. Through a comparative anatomical study of the leaflets, the botanical origins of the aerial part "Wei ling xian" drugs sold in Guangxi Province and Sichuan Province were proved to be C. uncinata CHAMP, and C. leiocarpa OLIV. (= C. uncinata var. coriacea PAMP.), resp...

  15. 大孔吸附树脂纯化东北铁线莲总黄酮的工艺研究%Purification technology of total flavonoids in Clematis florida Thunb.with macroporous resin method

    Institute of Scientific and Technical Information of China (English)

    王晓林; 钟方丽; 刘延超; 薛健飞

    2012-01-01

    Objective To establish the optimum purification conditions of total flavonoida in Clematis flanda Thunb. With macroporous resin method. Methods The technological conditions of purification were examined by the static adsorption and desorption experiments as well as dynamic adsorption and desorption tests, and took desorption rate as the main indicator. Results Total flavonoids in Clematis florida Thunb. Could be well purified with D-101 macroporous resin. On the condition that the concentration of total flavonoids was equal to the 0.2 g/ml raw material, the adsorption rate was 2 BV/h, concentration of ethanol was 95 % , desorption rate was 2 BV/h, and the pH value was 4, the content of eluant was 8 times of the raw material. After purified with macroporous resin method, the purity of Clematis florida Thunb. Was improved from 8.39% to 24.66%. Conclusion The results showed that total flavonoids in Clematis florida Thunb- can be well purified with D-101 macroporous resin.%目的 筛选适合纯化东北铁线莲总黄酮的大孔吸附树脂工艺.方法 采用静态吸附/解吸与动态吸附/解吸相结合的方法,以解吸率为主要指标考察各因素对东北铁线莲总黄酮大孔吸附树脂纯化工艺的影响.结果 6种树脂中D-101型大孔吸附树脂最适合纯化东北铁线莲总黄酮,其最佳工艺为:上样药液东北铁线莲总黄酬浓度相当于原生药0.2g/ml,吸附速率为2 BV/h,解吸液乙醇浓度为95%,解吸速率为2 BV/h,最佳上柱药液pH值为4,洗脱剂用量为8倍柱体积.经大孔吸附树脂分离纯化后,东北铁线莲总黄酮的纯度由8.39%提高到24.66%.结论 D-101型大孔吸附树脂对东北铁线莲总黄酮具有较好的纯化效果.

  16. 铁线莲属一新分类系统%A new system of classification of the genus Clematis(Ranunculaceae)

    Institute of Scientific and Technical Information of China (English)

    王文采; 李良千

    2005-01-01

    提出毛茛科Ranunculaceae铁线莲属Clematis一新分类系统.首先,简要回顾了此属的分类学研究历史,继对此属的营养器官和生殖器官的重要形态特征和花粉特征进行了分析,揭示出一系列演化趋势.这些趋势,尤其是萼片和雄蕊的演化趋势说明在现存的铁线莲属植物中,绣球藤Clematis montana与其少数近缘种的花构造最接近比铁线莲属原始的银莲花属Anemone,因此,包括绣球藤等植物的绣球藤组sect.Cheirpsis被认为是铁线莲属的原始群.主要根据花构造对铁线莲属现存的15组的亲缘关系进行了分析,发现它们是在4条演化干(绣球藤干、欧洲铁线莲干、尾叶铁线莲干、长瓣铁线莲干)的演化过程中先后形成的.本文将此4条演化干处理为亚属.最后做出属下各级分类群的系统排列,并给出简要的形态特征.

  17. New compound, 5-O-isoferuloyl-2-deoxy-D-ribono-γ-lacton from Clematis mandshurica: Anti-inflammatory effects in lipopolysaccharide-stimulated BV2 microglial cells.

    Science.gov (United States)

    Dilshara, Matharage Gayani; Lee, Kyoung-Tae; Lee, Chang-Min; Choi, Yung Hyun; Lee, Hak-Ju; Choi, Il-Whan; Kim, Gi-Young

    2015-01-01

    Microglia are main immune cells to exacerbate neural disorders in persistent overactivating. Therefore, it is a good strategy to regulate microglia for the treatment of neural disorders. In the present study, we isolated and characterized a novel compound, 5-O-isoferuloyl-2-deoxy-D-ribono-γ-lacton (5-DRL) from Clematis mandshurica, and evaluated its anti-inflammatory effect in lipopolysaccharide (LPS)-treated BV2 microglial cells. 5-DRL inhibited the expression of LPS-stimulated proinflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2), as well as their regulatory genes inducible NO syntheses (iNOS) and cyclooxygenase-2 (COX-2). 5-DRL also downregulated the LPS-induced DNA-binding activity of nuclear factor-κB (NF-κB) through suppression of the nuclear translocation of the NF-κB subunits, p65 and p50. Consistent with the inhibition of iNOS and COX-2 via NF-κB activity with 5-DRL, an inhibitor of NF-κB, pyrrolidine dithiocarbamate (PDTC), also led to the suppression of LPS-induced iNOS and COX-2 expression. Additionally, 5-DRL corresponding with antioxidants, N-acetylcysteine (NAC) and glutathione (GSH), remarkably inhibited reactive oxygen species (ROS) generation. Both NAC and GSH, thus attenuated the expression of iNOS and COX-2 by suppressing NF-κB activation, indicating that 5-DRL suppresses LPS-induced iNOS and COX-2 expression through downregulation of the ROS-dependent NF-κB signaling pathway. The present study also indicated that 5-DRL suppresses NO and PGE2 production by inducing heme oxygenase-1 (HO-1) via nuclear factor erythroid 2-related factor 2 (Nrf2). Taken together, the present data indicate that 5-DRL attenuates the production of proinflammatory mediators such as NO and PGE2 as well as their regulatory genes in LPS-stimulated BV2 microglial cells by inhibiting ROS-dependent NF-κB activation and stimulating the Nrf2/HO-1 signal pathway. These data may be implicated in the application of 5-DRL in LPS

  18. The Flower Germplasm Resources Habitats and Ornamental Types of Clematis in Yunnan%云南铁线莲花卉种质资源生境及观赏类型

    Institute of Scientific and Technical Information of China (English)

    唐岱; 李宗艳; 王锦

    2002-01-01

    云南铁线莲(Clematis)有56种,据其生境特点可分为亚热带森林型、暖温带森林型、温带森林型和林缘广布型;据花的形态特征和生长习性分可为常绿型、薄萼型和厚萼型;据花期可分为春花型、春末夏初型、夏花型、秋花型、冬花型.对种质资源的进一步开发利用作了讨论并提出建议.

  19. 国产毛茛科银莲花族十七种植物的细胞学研究%Cytology of ten species in Anemone,one in Anemoclema and six in Clematis (Trib. Anemoneae, Ranunculaceae) from China

    Institute of Scientific and Technical Information of China (English)

    杨亲二

    2002-01-01

    研究了国产毛茛科银莲花族Trib. Anemoneae 17种植物的染色体数目和核型.10种银莲花属Anemone L.植物中,1种(西南银莲花A. davidii)为x=8的四倍体(2n=4x=32),5种 (匍枝银莲花A. stolonifera、草玉梅A. rivularis、卵叶银莲花A. begoniifolia、水棉花 A. hupehensis f. alba、大火草A. tomentosa)为x=8的二倍体 (2n=2x=16), 4种 (鹅掌草A. flaccida、湿地银莲花A. rupestris、蓝匙叶银莲花A. trullifolia var. colestina、拟条叶银莲花A. trullifolia var. holophylla、展毛银莲花A. demissa) 为x=7 的二倍体 (2n=2x=14).罂粟莲花 Anemoclema glaucifolium为x=8 的二倍体.6种铁线莲属Clematis L. 植物 (滇川铁线莲C. kockiana、长花铁线莲 C. rehderiana、毛茛铁线莲 C. ranunculoides、扬子铁线莲C. puberula var. ganpiniana、短尾铁线莲 C. brevicaudata、金毛铁线莲 A. chrysocoma)均为 x=8的二倍体.银莲花属中x=7的种类的核型彼此十分相似,均由6对大型具中部着丝点的染色体和1对具端部着丝点的染色体组成;x=8的二倍体种类的核型与罂粟莲花属和铁线莲属植物的核型十分相似,均由5对大型具中部着丝点和3对具端部或近端部着丝点的染色体组成.%The somatic chromosome number and detailed chromosome morphology have been studied in ten species of Anemone, one species of Anemoclema, and six species of Clematis, all from China, namely Anemone davidii Franch. (2n=4x=32), A. stolonifera Maxim. (2n=2x=16), A. flaccida Fr. Schmidt (2n=2x=14), A. rivularis Buch.-Ham. (2n=2x=16), A. begoniifolia Lévl. et Vant. (2n=2x=16), A. hupehensis Lem. f. alba W. T. Wang (2n=2x =16), A. tomentosa (Maxim.) Péi (2n=2x=16), A. rupestris Hook. f. et Thoms. (2n=2x=14), A. trullifolia var. colestina (Franch.) Finet et Gagnep. (2n=2x=14), A. trullifolia var. holophylla Diels (2n=2x=14), A. demissa Hook. f. et Thoms. (2n=2x=14), Anemoclema glaucifolium (Franch.) W. T. Wang (2n=2x=16), Clematis kockiana Schneid. (2n=2x=16

  20. 铁线透骨草中槲皮素的提取条件优化及含量测定%Optimization of Extraction Conditions and Determination of Quercetin from Clematis Intricata Bunge

    Institute of Scientific and Technical Information of China (English)

    赵恒成; 耿凯凯; 李少坡; 汤彦丰; 李娜; 梁惠花; 甄攀

    2015-01-01

    Objective To study the optimal extraction conditions of quercetin from Clematis intricata Bunge by orthogonal test and determine contents of quercetin in different batches of Clematis intricata Bunge .Methods Setting the L9 (34 )orthogonal test table,arranging four factors:extraction tempera-ture,amount of solvent (methanol), extraction time and amount of hydrochloric acid.Detecting by HPLC;column:Hypersil C18 (5μm,4.6 mm × 250 mm),mobile phase:CH 3 OH-0.1% CH 3 COOH (50 ∶ 50),flow rate: 1.0 mL/min, column temperature: 25 ℃; determining wavelength: 375 nm. Results The optimal condition was that the sample with about 2.0 g conical flask with plug was poured 35 mL CH3 OH and 10 mL concentrated hydrochloric acid,then was soaked about 0.5 h,and extraced 40 min at the temperature of 40 ℃ with ultrasound concussion.Conclusion The extraction method could have high efficiency,short time and low temperature,and be better to extract quercetin from Clematis Intricata Bunge .The contents of quercetin could differ significantly in different batches of Clematis Intri-cata Bunge .%目的:利用正交试验研究铁线透骨草中槲皮素的最佳提取条件,测定不同批次铁线透骨草中槲皮素的含量。方法设置 L9(34)正交试验,安排提取温度、溶媒(甲醇)量、提取时间、加盐酸量4个因素, HPLC 法检测;色谱柱:Hypersil C18色谱柱(4.6 mm×250 mm,5μm);流动相:甲醇-0.1%乙酸(50∶50);流速:1.0 mL·min-1;柱温:25℃;检测波长:375 nm。结果最佳提取条件为:样品量约2.0 g,加入35 mL甲醇、10 mL 浓盐酸,浸泡0.5 h,于40℃超声震荡提取40 min。在此条件下测定的8个批次铁线透骨草中槲皮素的含量为0.1099~0.8803 mg·g-1。结论提取方法效率高、时间短、温度低,是提取铁线透骨草中槲皮素较好的方法。不同批次铁线透骨草中槲皮素的含量相差较大。

  1. Effect of Different Concentration PEG Simulating Drought Stress on the Clematis heracleifolia%不同浓度PEG模拟干旱胁迫对大叶铁线莲的影响

    Institute of Scientific and Technical Information of China (English)

    张磊; 孙慧颖; 高荣; 刘仁静; 岳桦

    2012-01-01

    大叶铁线莲具有耐旱植物特征,根系长达60cm以上。为研究其是否能够在大庆草原沙土干旱生境应用于园林绿化,选择大叶铁线莲一年生实生苗为试材,以30%、50%、70%浓度的PEG溶液人工模拟干旱渗透胁迫,测定其在不同干旱胁迫下,叶面积、叶片含水量、根系活力的变化。结果表明:大叶铁线莲的叶面积和叶片的绝对含水量与PEG的浓度及PEG的处理时间呈负相关:其中以30%PEG处理12h叶片开始出现皱缩,叶面积减少16.67%;50%PEG处理8h减少21.69%;70%PEG处理4h减少34.00%。根系活力与PEG的浓度及PEG的处理时间呈负相关,胁迫28h,30%、50%、70%PEG处理的根系活力分别下降53.36%、72.25%和81.62%。大叶铁线莲在不同干旱胁迫下具有一定的抗旱能力,30%PEG胁迫20h以内,70%植株能恢复,PEG对恢复后的植株仍有伤害,表现为形态不正常,但能长出新叶;50%PEG胁迫16h以内,65%植株能恢复;70%PEG胁迫12h植株就失去生命活力。复水28h后,30%、50%PEG根系活力各恢复44%和14%;70%PEG处理的根系活力没有恢复。%Clematis heracleifolia has drought-tolerant plants characteristics with the root over 60 cm.For the purpose of studying whether it could be used for landscaping in area of drought sand habitat like Daqing grassland,one-year Clematis heracleifolia was chosen as test materials and 30%,50%,70% PEG simulation drought osmotic stress were used to determine the leaf area,leaf water content,root vigor changes under different drought stress.The results showed that:the leaf area and leaf water content of Clematis heracleifolia had negative correlation with the concentration of the PEG and PEG processing time:30% PEG processing 12 h,leaves began to appear shrink,leaf area reducing 16.67%;50% PEG processing 8 h reduce 21.69%,and 70% PEG processing 4 h reduced by 34.00%.Root vigor has negative correlation with the concentration of the PEG and PEG processing time.Stressing 28 h

  2. Molecular identification of radix et rhizoma clematis and its adulterants based on ITS2 DNA barcode%基于ITS2条形码的中药材威灵仙与其易混伪品的鉴定

    Institute of Scientific and Technical Information of China (English)

    曾旭; 李莉; 业宁; 姚辉; 刘志华; 秦民坚

    2011-01-01

    Objective To discriminate radix et thizoma clematis and its adulterants. ancl also assure the quality and clinicalsafety of this traditional Chinese medicinal material. Methods In the study,ITS2 sequence of the clematis and its adulterants were evaluated according to sequence length, GC content,intra - specific variation. as well as the inter - specific divergence. NJ tree of radix et thizoma clematis its adulterants had heen constructed using MEGA4 software. The IT52 secondary structure was predicted using the ITS2 database and website constructed by Schultz et al. Results The length of ITS2 sequence of the origin plants of radix et thizoma clematis were 220 bp and 230 bp, respectively. ; In the phylogenetic tree ,the three origin plants could be clustered together, and the favorability rating was 85 % . From the branches of the plants, the distrihution center of the clematis genera in southwesten China conformed with the theory of center spread periphery. The difference between radix et rhizoma clematis and other species could be found in the number, size, position of loop and the angle of helix exsertion based on comparing the ITS2secondary structure of the origin plants of radix et rhizoma clematis and its adulterants. Conclusion ITS2c:an be used to correctly identify radix et thizoma clematis and its adulterants, and it plays an important role in the identification of traditional Chinese medicine.%目的 对中药材威灵仙及其易混伪品进行分子鉴定,为控制药材质量稳定和指导临床合理用药提供科学依据.方法 以核基因ITS2片段作为DNA条形码,对威灵仙基源植物及其易混品间的种内,种间序列差异进行分析比较,并构建NJ(邻接)树.通过Schultz等建立的ITS2数据库图及其网站预测ITS2二级结构.结果 威灵仙基源植物ITS2序列长度分别为220 bp和230 bp,由所构建的NJ树图可以看出,威灵仙、棉团铁线莲、东北铁线莲聚在一起,支持率达83%;由三种植物

  3. Leaf Epidermal Feature in Clematis (Ranunculaceae) with Reference to Its Systematic Significance%毛茛科铁线莲属的叶表皮特征及其系统学意义

    Institute of Scientific and Technical Information of China (English)

    史京华; 李良千

    2003-01-01

    用光学显微镜和扫描电镜,较全面地观察了铁线莲属(Clematis)植物和邻近类群的叶表皮特征,并对其系统学意义进行了讨论.在光学显微镜下,观察了铁线莲属12组63种5变种及其同族邻近类群3属9种;在扫描电镜下,观察了铁线莲属7组27种2变种及其邻近类群2属6种植物的成熟叶表皮细胞及气孔器的特征.在光学显微镜下可见:叶表皮细胞形状(表面观)为多边形、不规则形,垂周壁式样为较平直、浅波状和波状,气孔器普遍存在于下表皮,少数种的上表皮也有分布,其类型均为无规则型.在扫描电镜下可见:叶片通常具有条纹或有颗粒状或鳞片状附属物,气孔器外拱盖内缘通常为浅波状或近平滑.在黄花铁线莲组(sect. Meclatis (Spach) Tamura)和灌木铁线莲组(sect. Fruticella Tamura)中,叶表皮具有双面气孔器的特征对于组下亚组的划分有一定意义.并且叶表皮特征对于一些种的分类学处理也有一定的价值,同时也为属下组间的系统进化关系提供了一些线索.%For finding valuable systematic characters, leaf epidermis of 77 taxa, representing 12 sections of the genus Clematis and three related genera in the Ranunculaceae, were examined mainly by means of light microscopy (LM), and partially by scanning electron microscopy (SEM). It was shown that the leaf epidermal cells were usually irregular or polygonal in shape. The patterns of anticlinal walls were straight, arched or undulate. The stomatal apparatus is anomocytic and exists in abaxial epidermis of all species, and in the adaxial epidermis of some species. Under SEM observation, the leaf epidermis was often striated, seldom nearly smooth, and often with flakes attached. Evidence from leaf epidermis serves as a criterion for distinguishing the subsections in sect. Meclatis (Spach) Tamura and in sect. Fruticella Tamura. The results also support that there are several separate evolutionary processes in

  4. Cladistic Analysis of Clematis sect. Campanella Tamura (Ranunculaceae) from Morphological Characteristics%铁线莲属尾叶铁线莲组(毛茛科)基于形态学证据的分支系统学

    Institute of Scientific and Technical Information of China (English)

    孙诚; 谢磊; 李良千

    2007-01-01

    选取铁线莲属(Clematis)尾叶铁线莲组(sect.Campanella)中37个种以及西南铁线莲组(sect.Bebaeanthera)中的2个种为内类群,以Clematis alternata作为外类群,通过对全世界10个标本馆的近2 000份腊叶标本的形态学特征统计,选取了35个性状进行编码,利用PAUP 4.0 beta 1 0软件进行系统发育重建.通过最简约法(maximum parsimony)分析共得到1 82棵最简约分支树,树长为1 82步,一致性指数(CI)=0.385,保持性指数(RI)=0.685.结果表明:(1)尾叶铁线莲组并非是一个单系类群;(2)以花序发生位置这一性状建立的sect.Bebaeanthera不能成立,应并入尾叶铁线莲组;(3)本研究结果不支持在尾叶铁线莲组中建立subsect.Henryianae或ser.Henryianae;(4)C.ranunculoides等萼片外面具纵翅的一群植物与本组中萼片红色的种类C.lasiandra和C.dasyandra有较近的亲缘关系;(5)C.otophora、C.pogonandra、C.repens和C.barbellata等几个种聚为一支,且支持率很高,它们具有一系列的共衍征,即萼片质地较厚,花丝扁平,宽条形,被短柔毛,花药被黄色短毛,药隔先端凸起,因此不支持建立Ser.Pogonandrae;(6)本组中非洲分布的2个种无论从形态上还是从地理分布和生境上都十分特殊,是本组植物的特化类群.

  5. Comparative Study Between Effects of Smilax sieboldii Miq., Clematis chinensis Osbeck.and C.hexapetala Pall.on Non-specific Immunity%粘鱼须威灵仙棉团铁线莲对非特异性免疫的影响比较

    Institute of Scientific and Technical Information of China (English)

    石强

    2011-01-01

    Objective To compare the effects of Smilax sieboldii Miq. , Clematis chinensis Osbeck. and C. hexapetala Pall, on non-specific immunity, and further determine the feasibility of Smilax sieboldii Miq. to be used as drug.Methods The carbon granules clearance test in mice and chicken red blood cell phagocytosis test were carried out to compare the effects. Results All of Smilax sieboldii Miq. , Clematis chinensis Osbeck. and C.hexapetala Pall,inhibited the phagocytic function of monocyte-macrophage and abdominal cavity macrophage in mice, and reduced non-specific immune function.Conclusion Smilax sieboldii Miq. can be used equivalently as Clematis chinensis Osbeck. for the treatment of rheumatism.%目的 比较粘鱼须、威灵仙、棉团铁线莲对非特异性免疫的影响,为粘鱼须能否作为威灵仙入药提供科学依据.方法 选择小鼠碳粒廓清法和腹腔巨噬细胞吞噬鸡红细胞法进行比较3种药物.结果 粘鱼须、威灵仙、棉团铁线莲均能抑制小鼠单核巨噬细胞和腹腔巨噬细胞的吞噬作用,降低机体非特异性免疫反应能力.结论 粘鱼须可以作为祛风湿药威灵仙使用.

  6. 粘鱼须威灵仙和棉团铁线莲对细胞免疫与体液免疫的影响比较%Comparing Study of the Effects of Smilax sieboldii Miq., Clematis chinensis Osbeck. and C. hexapetela Pall on Cellular Immunity and Humoral Immunity

    Institute of Scientific and Technical Information of China (English)

    石强

    2011-01-01

    Objective To compare the effects of Smilax sieboldii Miq. , Clematis chinensis Osbeck. and C. hexapetela Pall on cellular and humoral immunity, and further determine the feasibility of Smilax sieboldii Miq. to be used as a drug. Methods Three cellular immune indexes including the formation rate of erythrocyte rosette, amount of T cell subsets and effects on delayed type hypersensitivity; two humoral immnue ones including the number of plaque forming cell ( PFC) and amount of serum hemolysin were compared. Results All of Smilax sieboldii Miq. , Clematis chinensis Osbeck. and C. hexapetela Pall decoction were able to inhibit the activity of T-lymphocyte and reduce the humoral immune function. Conclusion Smilax sieboldii Miq. can be used equivalently as Clematis chinensis Osbeck. for the treatment of rheumatism.%目的 比较粘鱼须、威灵仙、棉团铁线莲对细胞免疫和体液免疫的影响,为粘鱼须作为威灵仙入药提供科学依据.方法 选择影响玫瑰花环形成、T细胞亚群数量、迟发型变态反应等3个细胞免疫的指标和影响抗体形成细胞数量、血清溶血素数量等2个体液免疫的指标进行比较.结果 粘鱼须、威灵仙、棉团铁线莲均能抑制T细胞功能,降低机体细胞免疫反应能力.结论 粘鱼须可以作为祛风湿药威灵仙使用.

  7. Effects of Plant Growth Regulators on Cuttings of Rooting and Physiology of Clematis ‘Andromeda’%不同植物生长调节剂对铁线莲‘Andromeda’扦插生根及生理的影响

    Institute of Scientific and Technical Information of China (English)

    王磊; 高小慧; 曹丽娜; 陈坤

    2014-01-01

    以铁线莲品种 Clematis (Andromeda)半木质化枝条为材料,分别用不同浓度的萘乙酸(NAA)和吲哚丁酸(IBA)进行处理,清水作对照,研究不同植物生长调节剂对铁线莲的扦插生根能力和生根过程中 POD、SOD、PPO 氧化酶活性的影响。结果表明:清水处理的插穗生根率最高为32.27%,明显高于植物生长调节剂处理的插穗。铁线莲扦插生根过程中 POD、SOD、PPO 酶活性也随扦插生根不同表现出升高或降低的动态变化,其中对照组插穗的酶活性普遍较高。%As experimental materials,semi-lignified branches of Clematis ‘Andromeda’were treated with acetic acid (NAA)and indole butyric acid (IBA)by using different pH indicator.Compared with treatment by only u-sing fresh water,the research was focused on the effects of plant growth regulators on the cuttings of rooting of Clematis (Andromeda)and enzyme activity of POD,SOD and PPO during the cuttings of rooting process.The result showed that the highest rate of the cuttings of rooting of Clematis (Andromeda)by using fresh water was 32.27 %,which was much higher than the rate of using the plant growth regulators.During the cuttings of roo-ting process,the enzyme activity of POD,SOD and PPO showed dynamic changes,in terms of rising or reduc-ing.Generally in the control group the enzyme activity of POD,SOD and PPO during the cuttings of rooting process was high.

  8. Germplasm Resource of Wild Clematis Plants in Zhejiang Province and Application in Three-dimensional Greening%浙江省野生铁线莲属植物种质资源及其在立体绿化中的应用

    Institute of Scientific and Technical Information of China (English)

    吴冬

    2012-01-01

    介绍了浙江省野生铁线莲属植物的种质资源种类和分布,分析了它们的观赏特性及在立体绿化中的应用形式,并提出了其开发利用建议.%The germplasm resources and distribution of wild Clematis plants in Zhejiang Province were introduced, their ornamental features and application forms in three-dimensional greening were analyzed,and several advices for its development and utilization were put forward.

  9. Anti-inflammatory effects of Clematis chinensis Osbeck extract(AR-6) may be associated with NF-κB, TNF-α, and COX-2 in collagen-induced arthritis in rat.

    Science.gov (United States)

    Peng, Cheng; Perera, Pathirage Kamal; Li, Yun-Man; Fang, Wei-Rong; Liu, Li-Fang; Li, Feng-Wen

    2012-10-01

    The root of Clematis chinensis Osbeck has been used widely in rheumatoid arthritis in Chinese traditional medicine, and AR-6 is a triterpene saponin isolated from it. In this present study, we investigated the in vivo effects of oral AR-6 in chronic rat with collagen-induced arthritis (CIA) and possible molecular mechanism. CIA was induced by immunizing 56 female Sprague-Dawley (SD) rats with chicken typeIIcollagen (CII). Following eighteen days, the immunization rats with CIA were treated with AR-6 (32, 16, 8 mg/kg), cyclophosphamide (7 mg/kg), and TGP (Total Glucosides of Paeonia) (180 mg/kg) for 7 days, and rats without CIA were given the same volume of purified water. TNF-α and IL-1β levels in peripheral blood will be measured by ELISA, and Western blot analysis will be used to detect the expression of NF-κB p65 subunits, TNF-α and COX-2, in synovial membrane. We found that therapeutic treatment with AR-6 markedly improves the paw swelling and histopathological changes. Moreover, the serum levels of pro-inflammatory cytokines TNF-α and IL-1β were markedly lowered, and the expression of NF-κB p65 subunits, TNF-α and COX-2, in the synovial membrane of CIA rats was significantly inhibited in the AR-6-treated groups. These results enable to prove that AR-6 has a potential anti-inflammatory effect in CIA rats, and its mechanism may relate to the inhibition of the expression of NF-κB p65 subunits, TNF-α and COX-2. PMID:21932136

  10. Effect of Total Saponin from Clematis Chinensis Osbeck on the Differentiation Effect of Acute Promyelocytic Leukemia NB4 Cell Line in Vitro%威灵仙总皂苷诱导急性早幼粒细胞白血病细胞株NB4分化作用的研究

    Institute of Scientific and Technical Information of China (English)

    黄莉; 黄纯兰

    2012-01-01

    Objective:To study the effect of total saponin from clematis chinensis osbeck on differentiation of a-cute promyelocytic leukemia NB4 cell line cultured in vitro. Methods:The NB4 cells were independently treated with ATRA and total saponin combined with differemt concentrations in 5 days.The proliferative inhibition rates of NB4 cell induced by total saponin and ATRA were calculated in every day. Cell differentiations were demonstrated by Giemsa stain, transmission electron microscope (TEM) and NBT reduction assay at different time. The expressions of CD1lb and CD33 induced by these drugs were measured by flow cytometry (FCM) at different times. Results:As compared with blank control group,that the NB4 cells were inhibited significantly by the clematis total saponins showed a dose-effect dependence. There were no statistical significances in the differentiation phe-nomenons. Conclusion:The total saponin can obviously inhibit the proliferation of NB4 cells. However, there were no obvious differentiation effect in the NB4 cells treated by total saponin from clematis chinensis osbeck .%目的 研究威灵仙总皂苷对NB4细胞分化的影响.方法 取对数生长期的NB4细胞,设置多个浓度梯度的威灵仙总皂苷药物组,观察用药后不同时间点NB4活细胞数,计算生长抑制率;Wright's-Giemsa染色计数法和NBT还原试验测定细胞分化率,透射电镜观察细胞形态学变化;流式细胞术检测CD11b/CD33表达情况.结果 与空白对照组比较,威灵仙总皂苷对NB4细胞有明显的生长抑制作用,且具有剂量-效应依赖关系;但具有统计学意义的诱导分化现象不明显.结论 威灵仙总皂苷可有效抑制NB4细胞增殖,但对NB4细胞无明显的诱导分化作用.

  11. Род Clematis L. в Ботаническом саду г. Уфы

    OpenAIRE

    Насурдинова, Р.; Жигунов, О.

    2013-01-01

    В работе представлены результаты изучения сезонного ритма развития и зимостойкости 23 видов и 78 сортов рода Clematis L. в условиях интродукции в Ботаническом саду-институте г. Уфы. Боль-шинство видов и сортов клематиса проходят все стадии сезонного раз-вития. Проведена оценка декоративности сортов, выделены две груп-пы клематисов по декоративности – высокодекоративные (12 сортов) и декоративные (66 сортов). Изученные клематисы являются перспек-тивными для озеленения регионов Южного Урала....

  12. 威灵仙总皂苷体外抑制NB4细胞增殖及对CD11b、CD33表达的影响%Effect of total saponin from clematis chinensis osbeck on proliferation and CD116/CD33 expression of NB4 cells in vitro

    Institute of Scientific and Technical Information of China (English)

    黄莉; 李晓明; 黄纯兰

    2012-01-01

      目的:制备纯化的威灵仙总皂苷,体外作用于急性早幼粒细胞白血病NB4细胞,研究威灵仙总皂苷对NB4细胞分化的影响。方法:取对数生长期的NB4细胞,设置多个浓度梯度的威灵仙总皂苷药物组,观察用药后不同时间点NB4活细胞数,计算生长抑制率;Wright's-Giemsa染色计数法和NBT还原试验测定细胞分化率,透射电镜观察细胞形态学变化;流式细胞术检测CD11b/CD33表达情况。结果:与空白对照组比较,威灵仙总皂苷对NB4细胞有明显的生长抑制作用,且具有剂量-效应依赖关系;但具有统计学意义的诱导分化现象不明显。结论:威灵仙总皂苷可有效抑制NB4细胞增殖,但对NB4细胞无明显的诱导分化作用。%  Objective: To study the effect of total saponin from clematis chinensis osbeck on proliferation and differentiation of acute promyelocytic leukemia NB4 cell line in vitro.Methods:NB4 cells were treated with different concentration of total saponin for five days.The proliferation inhibition rates of NB4 cells induced by total saponin were calculated every day. Cell differentiation was demonstrated by Giemsa stain,transmission electron microscope(TEM) and NBT reduction assay at different time. The expressions of CD11b and CD33 induced by these drugs were measured by flow cytometry(FCM) at different time. Results: Compared with control group,clematis total saponin significantly inhibited the growth of NB4 cells dose dependently; but there was no statistical significance in cell differentiation. Conclusion: The total saponin can obviously inhibit the proliferation of NB4 cells, but has no significant role in cell differentiation.

  13. ПРОРАСТАНИЕ СЕМЯН И НАЧАЛЬНЫЕ ЭТАПЫ ОНТОГЕНЕЗА CLEMATIS BREVICAUDATA DC. И C. SERRATIFOLIA RENDER

    OpenAIRE

    Тонкова, Н.

    2010-01-01

    В статье приведены описания семян и начальных этапов онтогенеза двух видов рода клематис (Clematis brevicaudata и C. serratifolia). Выявлен ряд систематических признаков, облегчающих идентификацию видов на ранних этапах онтогенеза.

  14. CLEMATIS INTEGRIFOLIA И C. RECTA (RANUNCULACEAE) – РЕДКИЕ И НАХОДЯЩИЕСЯ ПОД УГРОЗОЙ ИСЧЕЗНОВЕНИЯ ВИДЫ ЛЕКАРСТВЕННЫХ РАСТЕНИЙ ТАМБОВСКОЙ ОБЛАСТИ

    OpenAIRE

    Яндовка, Людмила; Ярыгина, Марина

    2013-01-01

    Приводится ботаническое описание и применение двух редких видов – представителей рода Clematis, занесенных в Красную книгу Тамбовской области. Показаны лимитирующие факторы и необходимость сохранения Clematis integrifolia и C. recta во флоре Тамбовской области.

  15. МОРФОЛОГО-БИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА МУЖСКОЙ ГЕНЕРАТИВНОЙ СФЕРЫ CLEMATIS VITALBA L

    OpenAIRE

    ШЕВЧЕНКО С.В.; КУЗЬМИНА Т.Н.

    2015-01-01

    Проведено изучение процессов формирования мужских генеративных структур Clematis vi-talba L. Установлено, что особенности развития и строения элементов мужской генеративной сферы C. vitalba сходны с таковыми у других видов рода Clematis и некоторых представителей семейства Ranunculaceae. Основными эмбриологическими чертами C. vitalba является центростремительный тип формирования стенки микроспорангия, 2-3-рядный средний слой, секреторный тапетум, 2-клеточные пыльцевые зерна, образование больш...

  16. 低强度脉冲超声介导威灵仙干预兔膝关节软骨细胞增殖及Ⅱ型胶原和转化生长因子β1的表达%Effect of low-intensity pulsed ultrasound mediated Clematis chinensis Osbeck on the proliferation and expression of type II collagen and transforming growth factor-beta1 of rabbit knee articular chondrocytes

    Institute of Scientific and Technical Information of China (English)

    马勇; 郭杨; 屠娟; 成吉华; 郭各朴; 黄桂成

    2014-01-01

    BACKGROUND:Studies have shown that Clematis chinensis Osbeck can promote articular chondrocyte proliferation, maintain and promote the synthesis of cartilage proteoglycan and type II col agen in chondrocytes. Low-intensity pulsed ultrasound can effectively improve the carticular chondrocyte proliferation, increase the cellmembrane permeability, and promote chemicals or genes delivery, so as to improve the biological effects of chemicals. OBJECTIVE:To observe the effect of low-intensity pulsed ultrasound mediated Clematis chinensis Osbeck on the proliferation of cartilage cells and the expression of type II col agen and transforming growth factor (TGF)-β1 of rabbit knee articular chondrocytes cultured in vitro, and explore the effect and mechanism of Clematis chinensis Osbeck mediated by low-intensity pulsed ultrasound on cartilage damage. METHODThe rabbit knee articular chondrocytes were isolated and cultured in vitro. cells of the second generation in logarithmic growth phase were randomly divided into four groupcontrol group, low-intensity pulsed ultrasound (LIPUS) group, Clematis chinensis Osbeck (CCO) group, and CCO+LIPUS group. After cells were cultured under different conditions for 3 days, cellproliferation was measured with cellCount Kit-8 assay. The secretion of type II col agen was detected by cytochemical staining method, and the expression of TGF-β1 was assayed by western blot analysis. RESULTS AND CONCLUSION:The number of cells in the other three groups were significantly higher than that in control group at 3 days after culture (P  目的:观察低强度脉冲超声介导威灵仙对体外培养的兔膝关节软骨细胞增殖、Ⅱ型胶原及转化生长因子β1表达的影响,探讨低强度脉冲超声介导威灵仙在软骨损伤修复中的作用及机制。  方法:体外分离培养兔膝关节软骨细胞,取处于对数生长期的2代细胞,随机分为4组:对照组,低强度脉冲超声组,威灵仙组,威

  17. 湖州铁线莲乙醇提取物体外抗K562与MGC-803活性研究%Study on the Anti-tumor Activity of Ethanol Extracts of Clematis huchouensis Tamura on K562 and MGC-803 in vitro

    Institute of Scientific and Technical Information of China (English)

    卢莉娜; 赵伟春; 李丹; 卢蕾蕾; 余小波

    2012-01-01

    [目的]研究湖州铁线莲乙醇提取物体外抗肿瘤作用.[方法]干燥湖州铁线莲经石油醚脱脂、乙醇回流得到初提物.采用MTT法观察经AB-8树脂静态吸附70%乙醇解析处理后湖州铁线莲乙醇提取物体外对人白血病K562细胞及人胃癌MGC-803细胞的增殖抑制作用.[结果]在倒置显微镜下,湖州铁线莲处理组K562细胞形态不规则,MGC-803细胞体积缩小,部分细胞收缩变圆.两种细胞均生长缓慢,随着药物作用时间的延长,细胞的贴壁能力逐渐减弱,呈现出典型的凋亡形态学改变.MTT实验表明湖州铁线莲乙醇提取物对K562细胞及MGC-803细胞有增殖抑制作用,其作用68h的IC50值分别为133.66mg·L-1、317.87mg·L-1.[结论]体外实验中,湖州铁线莲乙醇提取物具有抗肿瘤作用,且呈现良好的浓度-效应依赖关系.%[Purpose] To observe the anti-tumor activity of the ethanol extracts of Clematis huchouensis Tamura in vitro. [Method] To obtain preliminary extracts, dry C. huchouensis were degreased with petroleum ether and refluxed with ethanol. In vitro, the inhibitory effects of ethanol extracts of C. huchouensis by static adsorption treatment on proliferation of human leukemia K562 cells and gastric carcinoma MGC-803 cells were measured by MTT eolorimetrie assay. [Result] The MTT test demonstrated that ethanol extracts of C. huchouensis inhibited proliferation of K562 cells and MGC-803 cells, and the IC50, values on them were 133. 66mg/L and 317.87mg/L, respectively. After treatment with ethanol extracts from C. huchouensis, the K562 cells grew slowly, turned irregular and the adherent ability decreased. While MGC-803 cells also grew slowly, the volume of cells became small, some cells condensed round masses, the adherent ability decreased, presenting a typical apoptosis morphological change. [Conclusion] In tumor inhibitory test, it was shown that the ethanol extracts of C. huchouensis may possess significantly inhibitory

  18. [The antitumour activity of total saponin of Clematis chinensis].

    Science.gov (United States)

    Qiu, G; Zhang, M; Yang, Y

    1999-07-01

    This paper has shown the cytocidal effects of total saponin of Clermatis chisensis Osbeck (CCS) on experimental tumours EAC cells, S180A cells and HepA cells in vitro, using microculture method 72 hours. The IC50 were 242 micrograms/ml, 193 micrograms/ml and 130 micrograms/ml respectively. In vivo the growth of transplanted mouse tumour(S180) was inhibited by the CCS with inhibitory rates 40.3% (0.25 g/kg), 55.1% (0.5 g/kg), 53.0% (1 g/kg), but CCS(0.2 g/kg, 0.4g/kg, 0.5 g/kg) hasn't effect on survival time of tumour mice(S180A). PMID:12571931

  19. СЕМЕННАЯ ПРОДУКТИВНОСТЬ ЛОМОНОСА ВОСТОЧНОГО (CLEMATIS ORIENTALIS L., RANUNCULACEAE) НА ЗАПАДНОМ ПРЕДЕЛЕ ЕГО РАСПРОСТРАНЕНИЯ

    OpenAIRE

    Смолянский, М.

    2012-01-01

    Излагаются результаты исследования Ломоноса восточного (Clematis orientalis L.) в пределах изолированной ценопопуляции, расположенной на территории Волгоградской области. Приводятся сведения о размерах, плотности и возрастной структуре ценопопуляции данного вида, также дается оценка динамики численности вида....

  20. In Vitro Propagation of Clematis filamentosa%甘木通的离体快繁

    Institute of Scientific and Technical Information of China (English)

    邵玲; 余淦新

    2005-01-01

    本研究初步建立快速培养甘木通组培苗的体系.以幼茎段为外植体,最适的诱导愈伤组织的培养基为MS+NAA 0.1 mg/L+6-BA 0.5 mg/L(单位下同);不定芽诱导和增殖的培养基为MS+NAA 0.05+6-BA 0.5,壮苗生根培养基为MS+NAA 0.1.经炼苗后,组培苗移栽成活率达78%左右.

  1. ОСОБЕННОСТИ НАЧАЛЬНЫХ ЭТАПОВ ОНТОГЕНЕЗА CLEMATIS INTEGRIFOLIA L. ПРИ ИНТРОДУКЦИИ В БОТАНИЧЕСКОМ САДУ - ИНСТИТУТЕ УФИМСКОГО НАУЧНОГО ЦЕНТРА РАН

    OpenAIRE

    БИЛАЛОВА РОЗА АЛЬТАФОВНА; НИКИТИНА ЛИЛИЯ САЛИХОВНА

    2015-01-01

    В статье дана характеристика биологических особенностей Clematis integrifolia L. при интродукции в Ботаническом саду в г. Уфе. По многолетним наблюдениям отмечено, что данный вид проходит все стадии сезонного развития: начало вегетации, цветение, плодоношение, окончание вегетации. Представлены результаты изучения начальных этапов онтогенеза C. integrifolia. Дана морфологическая характеристика семян. Описаны латентный период, состояние проростка, ювенильная, имматурная и виргинильная стадии. С...

  2. 威灵仙与柱果铁线莲体内外的高效液相色谱对比分析%Comparative Study on Clematis Chinesis and Clematis uncinata by HPLC in vitro and in vivo

    Institute of Scientific and Technical Information of China (English)

    王祥培; 钱海兵; 黄婕; 靳凤云; 孙宜春; 吴红梅

    2009-01-01

    目的 对常用中药材威灵仙及其地方品种柱果铁线莲体内外的高效液相色谱(HPLC)进行比较.方法 采用HPLC法分别测定威灵仙、柱果铁线莲、威灵仙含药血浆、柱果铁线莲含药血浆和空白血浆的指纹图谱,并分别进行威灵仙与柱果铁线莲、威灵仙含药血浆与柱果铁线莲含药血浆指纹图谱共有峰的分析.结果 威灵仙与柱果铁线莲药材HPLC指纹图谱有10个共有峰,威灵仙含药血浆与柱果铁线莲含药血浆HPLC指纹图谱有5个共有峰.结论 对威灵仙和柱果铁线莲体的体内外共有峰进行深入的比较分析,将有助于阐明具有相同功效的同属植物可能具有相同的药效物质基础.

  3. 铁线莲品种Clematis ‘Bill MacKenzie’愈伤组织的诱导%Callus Induction From Clematis ‘ Bill MacKenzie’

    Institute of Scientific and Technical Information of China (English)

    马育珠; 李林芳; 马玲玲; 王淑安; 汪庆; 李亚; 王鹏; 杨如同

    2015-01-01

    以铁线莲Clematis‘Bill MacKenzie’优良单株的嫩茎为外植体,研究了不同灭菌时间、基本培养基类型对C ‘Bill MacKenzie’愈伤组织诱导的影响,并在初代愈伤诱导的基础上,研究了不同外源激素组合对C ‘Bill MacKenzie’愈伤组织诱导及状态的影响.结果表明:最佳的灭菌时间为12 min;基本培养基类型为MS,诱导率为68.4%;不同的外源激素组合均能诱导出愈伤,但是愈伤状态不同,其中MS+1.0 mg/L6-BA+0.4 mg/L IBA时愈伤状态最好.

  4. Clematis属植物とその関連生薬の研究(第4報): 中国東北部産「威霊仙」の基源植物について

    OpenAIRE

    御影, 雅幸; 難波, 恒雄

    1983-01-01

    The origin of Chinese crude drug "Weilingxian" (威霊仙), available in market, is mainly derived from the underground portions of Clematis plants. To clarify the botanical origin of "Weilingxian" from northeast China, comparative anatomical study was made on the crude drug and the roots of five Clematis species growing wild in northeast China. Our results show that two Clematis species were recognized as the botanical origins of "Weilingxian" from this district, one was C. anguslifolia JACQ., and...

  5. 77 FR 34340 - Order Denying Export Privileges

    Science.gov (United States)

    2012-06-11

    ... most recent being that of August 12, 2011 (76 FR 50661, August 16, 2011), has continued the Regulations..., Inc., 102 Clematis Avenue, Suite 7, Waltham, MA 02453, Respondent, Chitron Electronics Company Limited..., Chitron Electronics, Inc. with a last known address at: 102 Clematis Avenue, Suite 7, Waltham, MA...

  6. О НОВЫХ И НАИБОЛЕЕ РЕДКИХ ВИДАХ ТАМБОВСКОЙ ФЛОРЫ. СООБЩЕНИЕ ВТОРОЕ

    OpenAIRE

    Соколов, А.; Соколова, Л.

    2006-01-01

    New and the most rare species of the flora of the Tambov Province. Report two. The article informs about the new (Glaucium corniculatum) and the most rare (Stipa borysthenica, Stipa zalesskii, Agropyron desertorum, Carex supine, Lychnis chalcedonica, Gypsophila altissima, Paeonia tenuifolia, Clematis integrifolia, Clematis recta, Dentaria quinquefolia, Anthyllis macrocephala, Euphorbia rossica, Euphorbia sareptana, Viola ambigua, Trinia multicaulis, Сephalaria litvinovii, Cirsium pannonicum, ...

  7. 長崎県植物誌ノ-ト(18)

    OpenAIRE

    中西, 弘樹

    1997-01-01

    Five species and two varieties including on alien plant which are newly found in Nagasaki Prefecture, and six rare species are described in this paper.Clematis williamsii is restricted on the calcareous sandstone area in the prefecture.

  8. 77 FR 27742 - Notice of Public Meetings for the Draft Environmental Impact Statement/Overseas Environmental...

    Science.gov (United States)

    2012-05-11

    ..., 2010, (75 FR 41163). The DoN's Proposed Action is to conduct training and testing activities--which may..., Pensacola, FL 32507. 28. West Palm Beach Public Library, 411 Clematis Street, West Palm Beach, FL...

  9. Clematis属植物とその関連生薬の研究(第5報): 台湾中部産および福建省産「威霊仙」の基源植物について

    OpenAIRE

    御影, 雅幸; 難波, 恒雄

    1983-01-01

    In central Taiwan, Chinese crude drug "Weilingxian" (威霊仙) with big roots, i.e., more than 5 mm in diameter, is available. "Weilingxian" derived from the underground portion of Clematis chinensis OSBECK of Ranunculaceae, which has many fibrous roots, is used commonly in this country. The small roots and lateral roots in the crude drug have some similarities fundamentally to the root of Clematis species having fibrous roots. Therefore, to clarify the botanical origin of this crude drug, the com...

  10. Clematis属植物とその関連生薬の研究(第7報): 「威霊仙」の本草学的考察(2)

    OpenAIRE

    御影, 雅幸; 難波, 恒雄

    1983-01-01

    As described in the previous paper, the first botanical origin of the Chinese crude drug "Weilingxian" was the Clematis plant of Ranunculaceae, especially the species in Sect. Tessen. But the botanical origin had changed with the times. In this paper, the change of the botanical origin of " Weilingxian" was clarified herbologically. As the result, the botanical origin of "Weilingxian" had changed from the species in Sect. Tessen to Clematis terniflora DC. in the Southern Song (南宋) dynasty of ...

  11. Study on the Isolation, Purification and Anti-tumor Activity in vitro of the Polysaccharides Extracted from Clematis Huchouensis Tamura%湖州铁线莲多糖的分离纯化及体外抗肿瘤活性研究

    Institute of Scientific and Technical Information of China (English)

    赵伟春; 李莉; 沈贵芳; 郭伟

    2011-01-01

    [Objective]To isolate and purify the polysaccharide extracted from C.huchouensis Tamura and study their vitro antitumor activities.[Methods]Polysaccharide from C.huchouensis, named CHTP, was separated and purified by DEAE-Cellulose 52 ion-exchange chromatograph and Sephadex G-200 gel chromatograph.The anti-tumor activities of CHTP and its purified products were studied by MTT colorimetry.Three tumor cells such as HepG2, MGC8-03 and K562 were selected to detect their inhibitory effect.[Results]A polysaccharide fraction, named CHTPⅡ-1, was harvested after purified.When the concentration of CHTP was 250~1000 mg· L- 1 or the concentration of CHTPⅡ-1 was 125~500 mg· L 1, the inhibition rate of CHTP or CHTPⅡ-1 on HepG2, MGC8-03 and K562 cells increased with the increase of the polysaccharide concentration.There were significant differences of the OD value between the polysaccharide treatments and negative control except for MGC8-03 cells treated with 62.5 mg·L-1 CHTPⅡ-1 or K562 cells treated with 125mg· L-1 CHTP tested by MTT.[Conclusion]Both CHTP and CHTPⅡ-1 can inhibit the proliferation of the three tumor cells.Moreover, the inhibition rate is positively correlated to the amount of polysaccharides in test concentration.%[目的]分离纯化湖州铁线莲粗多糖(CHTP)并测定其体外杭肿瘤活性.[方法]采用DEAE-cellulose 52离子交换层析和Sephadex G-200凝胶过滤层析分离纯化CHTP;采用MTT法测定CHTP及经纯化后多糖(CHTPII-1)对体外培养的人肝癌细胞HepG2、人胃癌细胞MGC8-03和人白血病细胞K562等三种肿瘤细胞的抑制作用.[结果]经分离纯化获得多糖组分CHTPII-1;在CHTP浓度为250~1000mg·L(-1)或CHTPII-1浓度为125~500mg·L(-1)的检测范围内,CHTP和CHTPII-1对HepG2、MGC8-03和K562三种细胞的抑制率均随着多糖浓度的增大而增大.除了MGC8-03细胞62.5mg·L(-1)CHTPII(-1)处理组和K562细胞125mg·L(-1)CHTP处理组的MTT检测OD值与阴性对照组间无显著差异外,其余各组的MTT检测OD值均显著或极显著低于阴性对照组.[结论]CHTP和CHTPII(-1)对HepG2、MGC8-03和K562等三种肿瘤细胞均有较强的抑制作用,且其抑制率与多糖浓度呈良好的量效关系.

  12. 威灵仙的原植物考查

    Institute of Scientific and Technical Information of China (English)

    黄颖雅

    2005-01-01

    威灵仙为毛茛科植物威灵仙Clematis chinensis Osbeck.、棉团铁线莲Clematis hexapetala Pall.、东北铁线莲Clematis manshurica Rupr.的干燥根茎。在中医临床上为常用中药,具祛风湿、通筋络、止痛之功效,在风湿痹痛、关节麻木、筋脉拘挛、骨哽咽喉方面疗效显著,应用较广。而在医药临床应用中,药材性状差异较大,笔者结合对有关文献的考证发现:全国有9个科23种商品的根以“威灵仙”之名人药。

  13. 威灵仙的成分、药理活性和临床应用的研究进展

    Institute of Scientific and Technical Information of China (English)

    赵燕强; 杨立新; 张宪民; 邱明华

    2008-01-01

    本文对中药威灵仙的有关研究进行了综述.主要为威灵仙(Clematis chinensis Osbeck)、棉团铁线莲(Clematis hexapetala Pall.)和东北铁线莲(Clematis manshurica Rupr.)成分中发现的化合物.包括皂甙、黄酮、挥发油等,并对新发现的药理作用进行阐述,也说明了治疗一些疾病的机制和起作用的化合物,还对威灵仙的临床应用进行了介绍.

  14. 9种铁线莲属药用植物的比较鉴别

    Institute of Scientific and Technical Information of China (English)

    胡玉涛; 周政昆

    2002-01-01

    @@ 毛茛科铁线莲属植物约有300种,我国有108种[1].其中作为正品威灵仙入药的为威灵仙Clematis chinesis Osbeck、棉团铁线莲Clematis hexapetala Pall.和东北铁线莲Clematis manshurica Rupr.的干燥根及根茎[2].目前市场上作为威灵仙流通的药材种类较多,而且性状相似,难以区别.现对9种铁线莲属药用植物的形态进行比较、鉴别,为今后的研究提供依据.

  15. 威灵仙种植技术

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    中药材威灵仙为毛茛科植物威灵仙Clematis chinensis Osbeck、棉团铁线莲(山蓼)Clematis hexapetala Pall.或东北铁线莲(黑薇)Clematis manshurica Rupr.的干燥根及根茎,别名铁脚威灵仙、百条根、老虎须、铁扫帚等,这3种植物为药典所规定的正品药材。性温,味辛、咸,归膀胱经;具有祛风除湿,通络止痛的功效;可用于风寒痹痛、四肢麻木、筋脉拘挛屈伸不利哽咽喉。

  16. Drug: D09111 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ca root [DR:D06768], Clematis root [DR:D06694], Sophora root [DR:D06710] Traditional Chinese Medicine in Japan [BR:br08304] Formulas... Formulas for external use Formulas for external use D09111 Jashoshito PubChem: 96025791 ...

  17. [Quality survey of different species of clematidis radix et rhizoma].

    Science.gov (United States)

    Li, Qian-Qian; Ma, Chang-Hua; Liu, Chun-Sheng; Xiao, Yao; Chen, Mei-Lan; Tian, Zhi-Hao; Wang, Yuan; Kong, Fan-Yao; Xu, Wen-Ying

    2013-04-01

    Quality survey of different species of Clematidis Radix et Rhizoma was made by determining the content of hederagenin and oleanolic acid from Clematidis Radix et Rhizoma. The result showed that only a few samples of Clematis chinensis met the quality standard for Clematidis Radix et Rhizoma in Chinese Pharmacopoeia 2010 Edition. PMID:23944036

  18. Five Newly Recorded Plant Species in Guizhou%贵州5个植物新纪录种

    Institute of Scientific and Technical Information of China (English)

    石磊; 胡小亮; 杨传东; 陈靖; 张泓; 杨妮

    2015-01-01

    首次报道贵州4个新纪录种和1个新纪录变种,蝶形花科(Papilionaceae)山豆根属(Euchres-ta)山豆根(Euchresta japonica Hook.f.ex Regel)、毛茛科(Ranunculaceae)毛果扬子铁线莲(Clematis ganpiniana var.tenuisepala (Maxim)C.T.Ting)和厚叶铁线莲(Clematis crassifolia Benth.)、芸香科(Rutaceae)茵芋属(Skimmia)的黑果茵芋(Skimmia melanocarpa Rehd.et Wils.)、爵床科(Acanthaceae)马蓝属(Pteracanthus Bremek)弯花马蓝(Pteracanthus cyphanthus (Diels)C.Y.Wu et C.C.Hu)。其中,三豆根为国家二级重点保护植物,首次发现在贵州有分布。%Four newly recorded species and one newly recorded varietas were first reported in Guizhou, including Euchresta japonica (Papilionaceae, Euchresta ), Clematis ganpiniana var. tenuisepala (Ranunculaceae),Clematis crassifolia (Ranunculaceae),Skimmia melanocarpa (Rutaceae,Skimmia), Pteracanthus cyphanthus (Acanthaceae,Pteracanthus).E.japonica is first reported in Guizhou,which is the national second-level key protected plants.

  19. 《中国药典》2010年版威灵仙不同品种常春藤皂苷元和齐墩果酸的含量探讨%The Chemical Markers for Quality Control in Different Species of Clematidis Radix et Rhizoma in Chinese Pharmacopoeia 2010 Edition

    Institute of Scientific and Technical Information of China (English)

    毕丹; 高明菊; 徐畅; 段然; 董婷霞; 詹华强

    2012-01-01

    目的:探讨《中国药典》2010年版中威灵仙的含量测定指标常春藤皂苷元和齐墩果酸的实用性.方法:优化《中国药典》2010年版威灵仙含量测定项下方法,并测定威灵仙中常春藤皂苷元和齐墩果酸的含量.结果:威灵仙Clematis chinensis Osbeck的药材符合《中国药典》2010年版的要求,常春藤皂苷元和齐墩果酸均大于0.30%;东北铁线莲Clematis manshurica Rup.r的药材中齐墩果酸大于0.30%,但常春藤皂苷元含量极微.结论:《中国药典》2010年版威灵仙的含量测定指标不能同时适用于威灵仙Clematis chinensis Osbeck和东北铁线莲Clematis manshurica Rupr.的质量控制,建议将两个品种含量测定指标分开制定.

  20. Study on Clematisnzans Hurica Rupr's Identification and Statographic Fingerprint Atlas%东北铁线莲的鉴别与色谱指纹图谱的研究

    Institute of Scientific and Technical Information of China (English)

    曹婷婷; 杨帆; 邢晶晶

    2011-01-01

    @@ 毛茛科铁线莲属植物约有300种.我国有108种.其中作为正品威灵仙人药的为威灵仙 Clematis chinesis Osbeck,棉团铁线莲 Clematishexapetala Pall 和东北铁线莲 Clematisnzans hurica Rupr的干燥根及根茎.

  1. 木通的品种、剂量与不良反应关系

    Institute of Scientific and Technical Information of China (English)

    宋雪英

    2001-01-01

    @@ 木通为兜铃科植物东北马兜铃(Aris to lochia manshuriensis kon)的干燥藤茎,商品名关木通,有清心火、利小便、通经下乳之功效;毛茛科植物小木通(Clematis armadiiFranch)或绣球藤(Clematis montana Buch - Ham)的干燥藤茎,商品名为川木通,具清热利尿、通经下乳的功效.以前者应用最广,后者次之.木通的来源较为复杂,除关木通、川木通外,尚有木通科植物互叶木通、三叶木通、白木通等.

  2. 蒙医用特木日-敖日秧古的本草考证与商品调查%Herbological Study of Temuri-aoriyanggu and Investigation of Goods

    Institute of Scientific and Technical Information of China (English)

    布日额

    2002-01-01

    据调查考证,蒙医用特木日-敖日秧古主要来源于2科2属9种植物。但《内蒙古蒙药材标准》确定的正品只有其中的细叶铁线莲Clematis aethusifolia Turcz.一种。

  3. 山木通挥发油化学成分分析

    Institute of Scientific and Technical Information of China (English)

    邱晓春; 靳凤云; 黄婕; 王祥培

    2009-01-01

    @@ 山木通Clematis finetiana Levl.et Vant.为毛茛科铁线莲属植物,主要分布于云南、贵州、四川、湖北、浙江等省,具有清热解毒、活血止痛、祛风除湿等功效,临床用于治疗感冒、膀胱炎、尿道炎等症~[1].

  4. Antisporulant Activity of Watery Extracts of Plants against Sclerospora graminicola Causing Downy Mildew Disease of Pearl Millet

    OpenAIRE

    S. A. Deepak; G. Oros; S. G. Sathyanarayana; H. S. Shetty; S. Sashikanth

    2007-01-01

    Watery extracts of forty plant species commonly growing in across India have been screened for antisporulant activity against Sclerospora graminicola (Sacc.) Schroet., the causative agent of pearl millet downy mildew. The collection represented 38 genera of 30 families. The extracts of thirteen species did not show any effect, whereas the activity of extracts of Allium sativum, Clematis gouriana, Evolvulus alsinoides, Mimusops elengi, Parthenium hysterophorus, Piper nigrum and Tagetes erecta ...

  5. Grapevine Yellows in the Republic of Macedonia: Molecular Identification of Stolbur Phytoplasma Strains in Grapevine and Weeds

    OpenAIRE

    Mitrev, Sasa; Karov, Ilija; Kostadinovska, Emilija

    2011-01-01

    During the period from 2006 to 2010, a survey for presence of Bois noir (BN) phytoplasmas of Vitis vinifera L., and wild spontaneous vegetation (Clematis vitalba L., Solanum nigrum L., Amaranthus retroflexus L., and Convolvulus arvensis L., was conducted. The aims of this study were: i)to check the presence of BN phytoplasmas on grapevines and wild vegetation in investigated vineyards in Eastern part of Macedonia, and II) to molecularly characterize and compare the isolates from grapevine wit...

  6. Lianen in mitteleuropäischen Pflanzengesellschaften und ihre Einnischung

    OpenAIRE

    Wilmanns, Otti

    2015-01-01

    Von den 88 in Mitteleuropa wild oder verwildert vorkommenden Lianen-Arten können Vitis vinifera und Humulus lupulus in Kulturen und die drei Wildpflanzen Clematis vitalba, Hedera helix und Rubus fruticosus agg. das Landschaftsbild prägen. Dies wird veranschaulicht. Die Verteilung der Kulturpflanzen auf Gesellschaftsklassen bzw. - Ordnungen gibt Tabelle 1 in Übersicht wieder. Als Lianen-reich erweisen sich: 1. die Mantel- und Heckengesellschaften der Rhamno-Prunetea, 2. die Saumgesellschaft...

  7. Flora survey on extensive meadows of selected area near Kobilje

    OpenAIRE

    Lainšček, Tadej

    2016-01-01

    Between the years 2009 and 2013, we mapped 273 different taxons on 70 polygones in extensively used grasslands near Kobilje village in Prekmurje. Amongs those taxons, there are 43 nature conservation important species: Achillea ptarmica, Alnus glutinosa, Alopecurus aequalis, Alopecurus pratensis, Carex acutiformis, C. appropinquata, C. canescens, C. tomentosa, C. umbrosa, C. vesicaria, Cerastium glutinosum, Clematis recta, Convallaria majalis, Dianthus carthusianorum, D. deltoides, D. superbu...

  8. Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype

    OpenAIRE

    Woudenberg, J.H.C.; Aveskamp, M.M.; Gruyter, de, J.; Spiers, A.G.; Crous, P.W.

    2009-01-01

    The fungal pathogen Phoma clematidina is used as a biological agent to control the invasive plant species Clematis vitalba in New Zealand. Research conducted on P. clematidina as a potential biocontrol agent against C. vitalba, led to the discovery of two perithecial-forming strains. To assess the diversity of P. clematidina and to clarify the teleomorph-anamorph relationship, phylogenetic analyses of 18 P. clematidina strains, reference strains representing the Phoma sections in the Didymell...

  9. Aurantiamide acetate suppresses the growth of malignant gliomas in vitro and in vivo by inhibiting autophagic flux

    OpenAIRE

    Yi YANG; Zhang, Li-hui; Yang, Bing-xian; Tian, Jin-kui; Zhang, Lin

    2015-01-01

    We aim to investigate the effect of aurantiamide acetate isolated from the aerial parts of Clematis terniflora DC against gliomas. Human malignant glioma U87 and U251 cells were incubated with different concentrations (0–100 μM) of aurantiamide acetate. Aurantiamide acetate greatly decreased the cell viability in a dose- and time-dependent manner. It induced moderate mitochondrial fragmentation and the loss of mitochondrial membrane potential. No significant difference was found in the altern...

  10. Clematichinenoside (AR) Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway

    OpenAIRE

    Haiyan Ding; Rong Han; Xueshan Chen; Weirong Fang; Meng Liu; Xuemei Wang; Qin Wei; Nandani Darshika Kodithuwakku; Yunman Li

    2016-01-01

    Mitochondria-mediated cardiomyocyte apoptosis is involved in myocardial ischemia/reperfusion (MI/R) injury. Clematichinenoside (AR) is a triterpenoid saponin isolated from the roots of Clematis chinensis with antioxidant and anti-inflammatory cardioprotection effects against MI/R injury, yet the anti-apoptotic effect and underlying mechanisms of AR in MI/R injury remain unclear. We hypothesize that AR may improve mitochondrial function to inhibit MI/R-induced cardiomyocyte apoptosis. In this ...

  11. 威灵仙化学成分和药理作用研究进展

    Institute of Scientific and Technical Information of China (English)

    李颜; 郭澄

    2008-01-01

    威灵仙为常用中药,具有祛风除湿、通络止痛的功效。2005年版《中国药典》(一部)记载其原植物来源为毛茛科铁线莲属植物威灵仙Clematis chinensis Osbeck、棉团铁线莲Clematis hexapetala Pall.和东北铁线莲Clematis mand-shurica Rupr的根及根茎。其单方和复方制剂在临床上广泛应用,主要治疗骨质增生、风湿性骨关节炎、肢体麻木和筋骨疼痛等病症。对威灵仙的研究已成为近期研究的热点。本文主要对威灵仙近10年来有关化学成分和药理作用的研究进行总结概述。

  12. Biological characteristics of 9 wild flower species seeds%9种野生花卉种子生物学特性研究

    Institute of Scientific and Technical Information of China (English)

    王荷; 郝爽; 刘燕

    2009-01-01

    该文研究了9种野生花卉的千粒重、含水量、种子形态及种子萌发特性.试验结果表明,在设定的温度范围15~25 ℃内,种子萌发难易度有明显不同.大花剪秋萝(Lychnis fulgens)和狭苞橐吾(Ligularia intermedia)较易萌发,发芽历程短,萌发的最适温度分别为25 ℃和20 ℃,发芽率分别达到97.3%和100%;芹叶铁线莲(Clematis aethusifolia)、短尾铁线莲(Clematis brevicaudata)和棉团铁线莲(Clematis hexapetala)发芽历程长,种子始发芽晚,在20 ℃条件下发芽率均达到最高,分别为88%,84%和57.3%.野罂粟(Papaver nudicaule)、牛扁(Aconitum barbatum var puberlum)、草本威灵仙(Veronicastrum sibiricum)和金莲花(Trollius chinensis)发芽率低,在试验条件下种子最高萌发率分别为29%,20%,17%和4.0%.

  13. ПРИМЕНЕНИЕ МЕТОДОВ БИОТЕХНОЛОГИИ ДЛЯ СОЗДАНИЯ СОРТОВЫХ КОЛЛЕКЦИЙ И ПОЛУЧЕНИЯ ПОСАДОЧНОГО МАТЕРИАЛА РОДА CLEMATIS L

    OpenAIRE

    Мякишева, Елена

    2013-01-01

    Рассматривается необходимость создания коллекций in vitro сортов клематисов для их комплексного исследования, сохранения генофонда и получения посадочного материала.

  14. Fungal Planet description sheets: 371-399.

    Science.gov (United States)

    Crous, P W; Wingfield, M J; Le Roux, J J; Richardson, D M; Strasberg, D; Shivas, R G; Alvarado, P; Edwards, J; Moreno, G; Sharma, R; Sonawane, M S; Tan, Y P; Altés, A; Barasubiye, T; Barnes, C W; Blanchette, R A; Boertmann, D; Bogo, A; Carlavilla, J R; Cheewangkoon, R; Daniel, R; de Beer, Z W; de Jesús Yáñez-Morales, M; Duong, T A; Fernández-Vicente, J; Geering, A D W; Guest, D I; Held, B W; Heykoop, M; Hubka, V; Ismail, A M; Kajale, S C; Khemmuk, W; Kolařík, M; Kurli, R; Lebeuf, R; Lévesque, C A; Lombard, L; Magista, D; Manjón, J L; Marincowitz, S; Mohedano, J M; Nováková, A; Oberlies, N H; Otto, E C; Paguigan, N D; Pascoe, I G; Pérez-Butrón, J L; Perrone, G; Rahi, P; Raja, H A; Rintoul, T; Sanhueza, R M V; Scarlett, K; Shouche, Y S; Shuttleworth, L A; Taylor, P W J; Thorn, R G; Vawdrey, L L; Solano-Vidal, R; Voitk, A; Wong, P T W; Wood, A R; Zamora, J C; Groenewald, J Z

    2015-12-01

    Novel species of fungi described in the present study include the following from Australia: Neoseptorioides eucalypti gen. & sp. nov. from Eucalyptus radiata leaves, Phytophthora gondwanensis from soil, Diaporthe tulliensis from rotted stem ends of Theobroma cacao fruit, Diaporthe vawdreyi from fruit rot of Psidium guajava, Magnaporthiopsis agrostidis from rotted roots of Agrostis stolonifera and Semifissispora natalis from Eucalyptus leaf litter. Furthermore, Neopestalotiopsis egyptiaca is described from Mangifera indica leaves (Egypt), Roussoella mexicana from Coffea arabica leaves (Mexico), Calonectria monticola from soil (Thailand), Hygrocybe jackmanii from littoral sand dunes (Canada), Lindgomyces madisonensis from submerged decorticated wood (USA), Neofabraea brasiliensis from Malus domestica (Brazil), Geastrum diosiae from litter (Argentina), Ganoderma wiiroense on angiosperms (Ghana), Arthrinium gutiae from the gut of a grasshopper (India), Pyrenochaeta telephoni from the screen of a mobile phone (India) and Xenoleptographium phialoconidium gen. & sp. nov. on exposed xylem tissues of Gmelina arborea (Indonesia). Several novelties are introduced from Spain, namely Psathyrella complutensis on loamy soil, Chlorophyllum lusitanicum on nitrified grasslands (incl. Chlorophyllum arizonicum comb. nov.), Aspergillus citocrescens from cave sediment and Lotinia verna gen. & sp. nov. from muddy soil. Novel foliicolous taxa from South Africa include Phyllosticta carissicola from Carissa macrocarpa, Pseudopyricularia hagahagae from Cyperaceae and Zeloasperisporium searsiae from Searsia chirindensis. Furthermore, Neophaeococcomyces is introduced as a novel genus, with two new combinations, N. aloes and N. catenatus. Several foliicolous novelties are recorded from La Réunion, France, namely Ochroconis pandanicola from Pandanus utilis, Neosulcatispora agaves gen. & sp. nov. from Agave vera-cruz, Pilidium eucalyptorum from Eucalyptus robusta, Strelitziana syzygii from

  15. Structural characterization and discrimination of Chinese medicinal materials with multiple botanical origins based on metabolite profiling and chemometrics analysis: Clematidis Radix et Rhizoma as a case study.

    Science.gov (United States)

    Guo, Lin-Xiu; Li, Rui; Liu, Ke; Yang, Jie; Li, Hui-Jun; Li, Song-Lin; Liu, Jian-Qun; Liu, Li-Fang; Xin, Gui-Zhong

    2015-12-18

    Traditional Chinese medicines (TCMs)-based products are becoming more and more popular over the world. To ensure the safety and efficacy, authentication of Chinese medicinal materials has been an important issue, especially for that with multiple botanical origins (one-to-multiple). Taking Clematidis Radix et Rhizoma (CRR) as a case study, we herein developed an integrated platform based on metabolite profiling and chemometrics analysis to characterize, classify, and predict the "one-to-multiple" herbs. Firstly, the predominant constituents, triterpenoid saponins, in three Clematis CRR were rapid characterized by a novel UPLC-QTOF/MS-based strategy, and a total of 49 triterpenoid saponins were identified. Secondly, metabolite profiling was performed by UPLC-QTOF/MS, and 4623 variables were extracted and aligned as dataset. Thirdly, by using pattern recognition analysis, a clear separation of the three Clematis CRR was achieved as well as a total number of 28 variables were screened as the valuable variables for discrimination. By matching with identified saponins, these 28 variables were corresponding to 10 saponins which were identified as marker compounds. Fourthly, based on the relative intensity of the marker compounds-related variables, genetic algorithm optimized support vector machines (GA-SVM) was employed to predict the species of CRR samples. The obtained model showed excellent prediction performance with a prediction accuracy of 100%. Finally, a heatmap visualization was employed for clarifying the distribution of identified saponins, which could be useful for phytochemotaxonomy study of Clematis herbs. These results indicated that our proposed platform was a powerful tool for chemical profiling and discrimination of herbs with multiple botanical origins, providing promising perspectives in tracking the formulation processes of TCMs products. PMID:26610614

  16. 威灵仙临床运用研究进展

    Institute of Scientific and Technical Information of China (English)

    刘自力; 赵荣

    2008-01-01

    威灵仙Clematis Chinensis Osbeck为毛莨植物威灵仙的根有效药理成分主要是原白头翁素和白头翁素,性辛咸、温、有毒、入膀胱经,功效主治为:祛风湿、通经络、消痰涎、散癖积。治痛风、顽痹、腰膝冷痛、脚气、疟疾、瘤瘕积聚、破伤风、扁挑体炎,治诸骨鲠咽。

  17. 女萎本草考证

    Institute of Scientific and Technical Information of China (English)

    吴晓燕

    2001-01-01

    女萎,萎蕤应是两个不同的品种。通过考证,认为《神农本草经》女萎、《名医别录》等女萎——萎蕤,是百合科植物玉竹Polygonatumodoratum (Mill.) Druce的根茎;《新修本草》等女萎,为毛茛科植物女萎Clematis apiifolia DC.的茎。

  18. 威灵仙临床新用

    Institute of Scientific and Technical Information of China (English)

    胡烈

    2001-01-01

    @@ 威灵仙为毛茛科植物威灵仙Clematis Chinensis Osbeck.棉团铁线莲C. herapetala pall.东北铁线莲C.等的根及根茎.又名:铁脚威灵仙、灵仙、黑脚威灵仙.喜生于山坡、沟边、路旁草丛中.主产于江苏、安徽、浙江等地.秋季采挖,去净茎叶,冼净泥土,晒干,或切成段晒于.生用或酒炒后用.

  19. Caryological notes in some portuguese Ranunculaceae

    OpenAIRE

    Queirós, Margarida

    1990-01-01

    Chromosome numbers of fourteen portuguese laxa of Ranunculaceae are reported: Helleborus foetidus 20 = 32; Nigella damascena 2n = 12; N. gallica 2n = 12; Delphinium Pentagynum 2n = 16; D. Halteratum subsp. verdunense 2n = 16; Anemone palmeta 2n = 32; Clematis campaniflora 2n = 16; Ranunculus muricatus 2n =...

  20. О НОВЫХ И НАИБОЛЕЕ РЕДКИХ ВИДАХ ТАМБОВСКОЙФЛОРЫ. СООБЩЕНИЕ 4

    OpenAIRE

    Соколов, Александр; Соколова, Людмила

    2014-01-01

    В работе сообщаются сведения о новых (Astragalus pubiflorus (Pall.)) DC., Veronica persica Poir., Dipsacus strigosus Willd. ex Roem. et Schult.) и наиболее редких (Ephedra distachya L., Stipa lessingiana Trin. et Rupr., S. pulcherrima C. Koch, Rhynchospora alba (L.) Vahl, Carex humilis Leysser, Veratrum nigrum L., Anthericum ramosum L., Lilium martagon L., Ceratocarpus arenarius L., Arenaria procera Spreng., Silene sibirica (L.) Pers., Actaea spicata L., Aconitum anthora L., Clematis integrif...

  1. 关木通川木通木通的鉴别比较

    Institute of Scientific and Technical Information of China (English)

    顾晓华

    2001-01-01

    @@ 历代本草所记载的木通为木通科的木通,目前很少见用;而临床目前使用最为广泛的是关木通,其次为川木通[1].下面将关木通、川木通、木通的鉴别特点作一比较. 1 来源 1.1 关木通为马兜铃科植物木通马兜铃Aristolochia manshuriensis Kom.的木质茎. 1.2 川木通为毛茛科植物小木通Clematis armandii Franch.或绣球藤Clematis montana Buch.-Ham的木质茎. 1.3 木通为木通科植物白木通Akebia trifoliata (Thunb.) Koidz.var.custralis (Diels) Rehd.或三叶木通 Akebia trifoliata (Thunb.) Koidz.或木通 Akebia quinata (Thunb.) Decne 的木质茎.

  2. Genetic diversity of European phytoplasmas of the 16SrV taxonomic group and proposal of 'Candidatus Phytoplasma rubi'.

    Science.gov (United States)

    Malembic-Maher, Sylvie; Salar, Pascal; Filippin, Luisa; Carle, Patricia; Angelini, Elisa; Foissac, Xavier

    2011-09-01

    In addition to the grapevine flavescence dorée phytoplasmas, other members of taxonomic group 16SrV phytoplasmas infect grapevines, alders and species of the genera Clematis and Rubus in Europe. In order to investigate which phytoplasmas constitute discrete, species-level taxa, several strains were analysed by comparing their 16S rRNA gene sequences and a set of five housekeeping genes. Whereas 16S rRNA gene sequence similarity values were >97.5 %, the proposed threshold to distinguish two 'Candidatus Phytoplasma' taxa, phylogenetic analysis of the combined sequences of the tuf, rplV-rpsC, rplF-rplR, map and uvrB-degV genetic loci showed that two discrete phylogenetic clusters could be clearly distinguished. The first cluster grouped flavescence dorée (FD) phytoplasmas, alder yellows (AldY) phytoplasmas, Clematis (CL) phytoplasmas and the Palatinate grapevine yellows (PGY) phytoplasmas. The second cluster comprised Rubus stunt (RS) phytoplasmas. In addition to the specificity of the insect vector, the Rubus stunt phytoplasma contained specific sequences in the 16S rRNA gene. Hence, the Rubus stunt phytoplasma 16S rRNA gene was sufficiently differentiated to represent a novel putative taxon: 'Candidatus Phytoplasma rubi'. PMID:20889771

  3. 西藏植物的新分布属和新分布种

    Institute of Scientific and Technical Information of China (English)

    于顺利; 李晖; 土艳丽

    2008-01-01

    根据西藏高原生物研究所标本馆馆藏标本,发现有西藏新分布属2个,即马甲子属(Paliurus)和菠萝蜜属(Arctocarpus);西藏新分布种23个,即马甲子(Paliurus ramossissimus)、扁担杆(Grewia biloba)、儿茶(Acacia catechu)、华南皂角(Gleditsia fera)、白背桤叶树(Clethra petelotii)、思茅铁线莲(Clematis ranunculoides var.pterantha)、疏果山蚂蝗(Desmodium griffithianum)、滇南山蚂蝗(Desmodium megaphyllum var.megaphyllum)、芹叶铁线莲(Clematis aethusifolia)、棱喙毛茛(Ranunculus trigonus)、中华水龙骨(Polypodiodes chinensis)、贡山猕猴桃(Actinidia pilosula)、长齿木蓝(Indigofera dolichochaeta)、线叶柳(Salix wihelmsiana var.wihelmsiana)、透茎冷水花(Pilea pumila var.pumila)等.发现栽培种及逸生种3个,即棒叶槭Acer negundo)、木槿(Hibiscus syriacus)和欧洲千里光(Senecio vulgaris).

  4. THE CICADA FAUNA AS PHYTOPLASMA VECTORS IN ISTRIAN VINEYARDS

    Directory of Open Access Journals (Sweden)

    Đanfranko Pribetić

    2009-12-01

    Full Text Available The cicada fauna represents a considerable group of insects in vine-growing. Phytoplasma vine vectors insects are significant. They are fed from the phloem tissues of plants like cicadas from the families Cicadelidae, Coccidae, Fulgoridae and Psyilloidaea. Their phytoplasma is transmitted in a persistent way. Researches on cicada fauna, on the floristic structure of weeds and host plants of vine phytoplasma were done in 2005 and 2006 in Istrian vineyards. The research was being done in 10 vineyards on 11 localities. Cicade collecting was done with an entomological net by means of yellow sticky plates using an exhauster and a method of clonting. The collected cicadas were identified by means of binoculars and keys to identify species while the presence of phytoplasmas BN and Fd was defined by means of molecular analysis (PCR, RFLP. Listing and identifying the floristic structure of weeds were being cloned in the explored vineyards by means of keys to identify species. Samples of plant materials were taken for the analysis using PCR method by checking visually the typical symptoms caused by phytoplasma. Cicadas identifying and molecular analyses were being done at the Viticulture Institute for Research in Conegliano – Italy. During the researches, 243 insect samples were collected. Of the above mentioned number cicadas of 40 genus were identified in 207 samples. On the list of the floristic structure of Weeds 105 species of 36 families were identified. Corylus avellana L and Clematis vitalba L species were included in this list. These species showed sigus of phytoplasma disease and they were found near the explored vineyards. These two species were analysed on the presence for FD and BN phytoplasmas. The PCR method used in the molecular research on the presence of Fd and BN phytoplasmas was done on 34 insect samples and 22 plant samples. None of the mentioned sample was positive for FD and BN. The phytoplasma BN was found in the vine leaves of

  5. Antigonorrhoeal activity of plants used in Guatemala for the treatment of sexually transmitted diseases.

    Science.gov (United States)

    Cáceres, A; Menéndez, H; Méndez, E; Cohobón, E; Samayoa, B E; Jauregui, E; Peralta, E; Carrillo, G

    1995-10-01

    Plants popularly used in Guatemala for the treatment of gonorrhoea were macerated in 50% alcohol and the tincture tested for in vitro activity against Neisseria gonorrhoeae using strains isolated from symptomatic patients and confirmed by standard bacteriological procedures. From 46 plants investigated, 13 (28.3%) showed evident inhibition zones (> 9 mm), seven (15.2%) showed small activity (6.1-8.9 mm) and 26 (56.5%) showed no activity; nine of these plants inhibited five strains of N. gonorrhoea freshly isolated. The most active plants of American origin were: bark of Bixa orellana fruits of Parmentiera edulis, leaf of Diphysa robinioides, Eupatorium odoratum, Gliricidia sepium, Physalis angulata, Piper aduncum and Prosopis juliflora, root of Casimiroa edulis, and whole Clematis dioica. PMID:8583798

  6. 甘木通人工栽培

    Institute of Scientific and Technical Information of China (English)

    苏战仪; 尹显宏

    2004-01-01

    甘木通Clematis filamentosa Dunn又名丝铁线莲、眼蛇药,为毛茛科常绿攀缘藤本,主要分布在广东、广西、福建、台湾、云南、海南等地,以广东、广西居多。广东的信宜、韶关、云浮、肇庆、河源等地有产,主产区在韶关。甘木通喜生于海拔400~1200m的山地沟谷,常攀缘于其他植物之上。

  7. 畲药十二时辰的本草考证

    Institute of Scientific and Technical Information of China (English)

    黄泽豪; 张月玲

    2014-01-01

    十二时辰是福建民间的一味畲药,文中对其名称,原植物及性味功效等进行了文献整理与考证.结果表明,十二时辰来源于毛茛科植物,为重瓣铁线莲Clematis florida Thunb.var.plena D.Don的干燥根,最早收载于明代兰茂编著的,福安民间畲医对十二时辰功用的认识及用药部位方面皆具有地方民族特色.

  8. 蒙药才棉团铁线莲化学成分和药理研究进展

    Institute of Scientific and Technical Information of China (English)

    巴达日呼; 房玉良

    2009-01-01

    蒙药才棉团铁线莲clematis hexapetala pall.具有破痞,调温, "协日乌紊",止腐,消肿,止泻之功效.临床上主治寒痞,消化不良,寒性"协日乌素"病,"吾雅曼"病,黄水疮,疮疡,水肿,寒泻,祛除各种瘤的功效.所含的化学成分黄酮类、糖类、皂苷类、白头翁索、生物碱、挥发油和药理作用抗肿瘤、降血压、降糖、抑菌,抗氧化等作用.

  9. THE VASCULAR FLORA OF HALE SCOUT RESERVATION: LEFLORE COUNTY, OKLAHOMA

    Directory of Open Access Journals (Sweden)

    Bruce W. Hoagland

    2010-12-01

    Full Text Available The Hale Scout Reservation is located in the Ouachita Mountains of southeastern Oklahoma, a region of high plant diversity in the state. A vascular plant inventory yielded 463 species of vascular plants in 288 genera and 99 families. The largest families were the Asteraceae (with 65 species and Poaceae (56. The flora consisted of 120 annuals, 1 biennial, and 342 perennials. Forty-two non-native species were collected, representing 8.8% of the flora. Sixteen species tracked by the Oklahoma Natural Heritage Inventory were present: Amorpha ouachitensis (S1, Aristolochia serpentaria (S1, Baptisia nuttalliana (S2, Brachyelytrum erectum (S1, Brasenia schreberi (S1, Carex ouachitana (S1, Chionanthus virginicus (S2, Clematis crispa (S1, Didiplis diandra (S1S2, Galium arkansanum (S2, Houstonia ouachitana (S1, Juncus repens (S1, Modiola caroliniana (S2, Monotropa hypopithys (S1, Muhlenbergia bushii (S1, and Ribes cynosbati (S2 (Oklahoma Natural Heritage Inventory, 2010.

  10. 铁线莲属植物的栽培与引种

    Institute of Scientific and Technical Information of China (English)

    李瑞国

    2004-01-01

    @@ 铁线莲属(Clematis L.)植物属毛莨科,广泛分布于世界各地,是野生美丽花卉之一,可供观赏;大部分铁线莲也是药用植物,其根、茎及全株皆可入药.日本、英国等西方国家对铁线莲的栽培育种进行了多年的研究,已培育出几百种不同花色、不同花型、不同开花季节的园艺品种.

  11. Inhibitory effects of Turkish folk remedies on inflammatory cytokines: interleukin-1alpha, interleukin-1beta and tumor necrosis factor alpha.

    Science.gov (United States)

    Yeşilada, E; Ustün, O; Sezik, E; Takaishi, Y; Ono, Y; Honda, G

    1997-09-01

    In this study, in vitro inhibitory effects of 55 extracts or fractions obtained from 10 plant species on interleukin-1 (IL-1alpha, IL-1beta) and tumor necrosis factor (TNF-alpha) biosynthesis were studied. The following plant materials from Turkish folk medicine for the treatment of various diseases which are thought to be inflammatory in nature e.g. rheumatism, fever, infections, edemas or related inflammatory diseases were selected as the subject of this study: Cistus laurifolius leaves, Clematis flammna flowering herbs, Crataegus orientalis roots, Daphne oleoides ssp. oleoides whole plant, Ecbalium elaterium roots, Rosa canina roots, Rubus discolor roots, Rubus hirtus roots, Sambucus ebulus flowers and leaves, Sambucus nigra flowers and leaves. All plants showed inhibitory activity against at least one of these models in various percentages depending upon the concentration, thus supporting the folkloric utilization. Daphne oleoides was found to be the most active plant against the test models. PMID:9324006

  12. 蒙药材芹叶铁线莲对海虾幼虫的致死活性

    Institute of Scientific and Technical Information of China (English)

    付明海; 阿丽沙; 包保全; 张屏; 赵德力格尔呼

    2011-01-01

    海虾幼虫(brine shrimp)致死性生物活性测定法是一种简单、快速、准确的生物测定法.对蒙药材芹叶铁线莲(Clematis aethusifolia)75%乙醇提取物的5个不同萃取部位和19个不同极性组分进行活性追踪研究.活性数据结果显示蒙药材芹叶铁线莲乙酸乙酯层低压反相制备柱85%加甲醇洗脱组分活性最强,LC50为6.7μg/mL.

  13. The phylogenetic significance of fruit structures in ranunculaceae of china

    International Nuclear Information System (INIS)

    The external and internal structures of fruits from 95 taxa representing 27 Ranunculaceae genera of China were studied. The results show that Ranunculaceae could be divided into 4 groups based on the fruit types, epidermal surface, vascular bundle, mesocarp cell, and endocarp cell structures: Group 1: follicle or achene, branching or branching and anastomosing vascular bundles, mesocarp parenchyma, and endocarp with one layer of lignified cells (including Aconitum and other genera); Group 2: achene, vascular bundle branching, mesocarp lignified, endocarp with one layer of irregular and partly lignified cells (Thalictrum only); Group 3: achene, endocarp with multilayered thick-walled cells (including Adonis, Batrachium and Ranunculus); Group 4: achene, two non-branching vascular bundles, and endocarp with one layer of fibers (including Anemone, Clematis and Pulsatilla). This study show that the fruit structures of Ranunculaceae could provide morphological and anatomical evidences for molecular phylogeny. (author)

  14. Analysis of the mating-type loci of co-occurring and phylogenetically related species of Ascochyta and Phoma.

    Science.gov (United States)

    Woudenberg, Joyce H C; De Gruyter, Johannes; Crous, Pedro W; Zwiers, Lute-Harm

    2012-05-01

    Ascochyta and Phoma are fungal genera containing several important plant pathogenic species. These genera are morphologically similar, and recent molecular studies performed to unravel their phylogeny have resulted in the establishment of several new genera within the newly erected Didymellaceae family. An analysis of the structure of fungal mating-type genes can contribute to a better understanding of the taxonomic relationships of these plant pathogens, and may shed some light on their evolution and on differences in sexual strategy and pathogenicity. We analysed the mating-type loci of phylogenetically closely related Ascochyta and Phoma species (Phoma clematidina, Didymella vitalbina, Didymella clematidis, Peyronellaea pinodes and Peyronellaea pinodella) that co-occur on the same hosts, either on Clematis or Pisum. The results confirm that the mating-type genes provide the information to distinguish between the homothallic Pey. pinodes (formerly Ascochyta pinodes) and the heterothallic Pey. pinodella (formerly Phoma pinodella), and indicate the close phylogenetic relationship between these two species that are part of the disease complex responsible for Ascochyta blight on pea. Furthermore, our analysis of the mating-type genes of the fungal species responsible for causing wilt of Clematis sp. revealed that the heterothallic D. vitalbina (Phoma anamorph) is more closely related to the homothallic D. clematidis (Ascochyta anamorph) than to the heterothallic P. clematidina. Finally, our results indicate that homothallism in D. clematidis resulted from a single crossover between MAT1-1 and MAT1-2 sequences of heterothallic ancestors, whereas a single crossover event followed by an inversion of a fused MAT1/2 locus resulted in homothallism in Pey. pinodes. PMID:22014305

  15. SPRING FLORA OF CEMETERIES OF ODESSA

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    SPRING FLORA OF CEMETERIES OF ODESSA

    2014-04-01

    Full Text Available The spring flora was analyzed on six cemeteries in Odessa, such cemeteries as Vtoroe xristianskoe, Troickoe, Tairovskoe, Trete evrejskoe, Oficerskoe (Dmitrievskое and Severnoe. There were found and identified 235 species of plants, which belong to 186 genera and 67 families. There was taken a taxonomic analysis of flora of the cemeteries also of spreding of plants by ekobiomorphs, the analysis of hronotyp and origin. The proportion of the flora on the cemeteries is 1:2,8:3,5. The dominant family of the flora of Odessa’s cemeteries is Asteraceae. There are other leading families such as Rosaceae, Fabaceae, Poaceae, Brassicaceae, Lamiaceae. Herbs and trees are dominant among the life forms. Mesophytes and kseromezophytes are in the majority among hihromorphs. Heliophytes are on the first place by adapting to the light. Our results showed that the adventitious plants occupy up to 44% of all amount of plants at the cemeteries in Odessa. Kenophytes is a dominant group among them. Floragenetics analysis revealed the dominance of the plants from Asia. There have been allocated plants that were met at all six cemeteries independently of the location religious and age characteristics of the cemetery. "Core" of the flora of Odessa’s cemetery have made weed Acer negundo L., Ballota nigra L. and decorative Hedera helix L., Centaurea dealbata Willd., Buxus sempervirens L., Convallaria majalis L., Sedum kamtschaticum Fisch., Thuja occidentalis L., Hemerocalis fulva (L. L. There were found 4 species of plants that belong to the rare and endangered plants of Odessa’s region: Convallaria majalis L., Hyacinthella leucophaea (K. Koch Schur, Clematis integrifolia L., Paeonia tenuifolia L. Moreover Convallaria majalis L. grows on all six investigated cemeteries. Also two species: Hyacinthella leucophaea (K. Koch Schur and Clematis

  16. ОСОБЕННОСТИ ВОДНОГО РЕЖИМА И ЖАРОСТОЙКОСТЬ КЛЕМАТИСОВ

    OpenAIRE

    Насурдинова, Роза; Жигунов, Олег

    2013-01-01

    В статье представлены результаты опытов по определению водоудерживающей способности листьев 12 видов рода Clematis L. и жаростойкости 11 видов и 1 сорта рода Clematis L. в условиях культуры в Башкирском Предуралье. Выявлены общие тенденции динамики водных показателей в течение вегетационного сезона, характеризующие адаптационный потенциал видов....

  17. Inhibitory Effects of Some Extracts in Tongfengning on Xanthine Oxidase%痛风宁部分提取物对黄嘌呤氧化酶的抑制作用

    Institute of Scientific and Technical Information of China (English)

    王晓梅; 赵岩; 赵卫星

    2012-01-01

    Objective To observe the inhibitory effects of some extracts in Tongfengning, a traditional Chinese medicine prescription for treating gout, on xanthine oxidase (XOD) and their antioxidant activity. Methods Uric acid was produced from xanthine in the presence of XOD. The reducing power was evaluated by the potassium ferricyanide reduction method, and the impacts of the extracts on the absorbance of the reaction system were determined. Results The ethyl acetate extract and ethanol extract of ash bark and the ethyl acetate extract of Chinese Clematis root showed the strongest inhibitory effects on XOD. The ethyl acetate extract of Chinese Clematis root showed the strongest reducing power. Conclusion Some extracts in Tongfengning have strong inhibitory effects on XOD and strong antioxidant activity in vitro.%目的 观察痛风宁部分提取物对黄嘌呤氧化酶(xanthine oxidase,XOD)的影响及抗氧化作用.方法 通过XOD催化黄嘌呤产生尿酸,采用铁氰化钾还原法测定还原力,分别测定各提取物对反应体系吸光度的影响.结果 秦皮乙酸乙酯提取物和秦皮乙醇提取物及威灵仙乙酸乙酯提取物对XOD的抑制作用最强,威灵仙乙酸乙酯提取物的还原能力最强.结论 痛风宁部分提取物具有较强的抑制XOD活性作用并具有较强的体外抗氧化活性.

  18. Extraction and Antibacterial Properties of Flavonoids from Wild Herbs%几种野菜中黄酮的提取及抑菌作用研究

    Institute of Scientific and Technical Information of China (English)

    丁存宝; 李桂秋; 刘海燕; 贾长虹; 张学祥; 常立新

    2012-01-01

    Flavonoid compound extensively existed to a second-class metabolism outcome of the polyphenol in plant had a lot of potential medicinal value. Antibacterial activity of flavonoid was the hot spots of study. The antibacterial activity of flavonoid from several wild herbs was tested by using the bacteria Bacillus subtilis, Escherichia coli, Salmonella typhi, fungi Penicillium, Saccharomyces cerevisiae as the tested strains. All the samples collected to isolate flavonoids from Lespedeza and Clematis Aethusifolia. The flavonoid extracts were diluted with double dilution method. Antibacterial activity of flavonoid was measured by taking inhibition zone method. The results showed that the antibacterial activity of flavonoid from several wild herbs: Escherichia coli〉Bacillus subtilis〉Salmonella typhi 〉Accharomyces〉Penicillium glaucum. The antibacterial activity was correspondent with the extract flavonoid's concentration. The MIC of Lespedeza and Clematis were 332.6μL/mL and 441.5 μL/mL, respectively. The antibacterial activity of flavonoids from Clematis was superior to Lespedeza.%黄酮类化合物是广泛存在于自然界中的多酚类植物次级代谢产物,具有许多潜在的药用价值和生物活性,其中抑菌活性是研究热点之一。以枯草芽孢杆菌(Bacillus subtilis)、大肠杆菌(Escherichia coli)、伤寒杆菌(Salmonella typhi)和青霉菌(Penicillium glaucum)、酿酒酵母(Accharomyces)为供试菌研究了几种野菜中黄酮提取物的抑菌作用。首先从芹叶铁线莲和胡枝子中分离提取黄酮提取液,用二倍稀释法制成不同浓度的黄酮溶液。再采取圆滤纸片法进行抑菌试验,测量出不同菌种和不同黄酮浓度下的抑菌圈直径。从试验结果得出野菜黄酮对供试菌的抑菌效果依次为:大肠杆菌〉枯草杆菌〉伤寒杆菌〉酿酒酵母〉青霉菌。结果表明抑菌活性与对应提取物中总黄酮有关,其含量直接影

  19. Preliminary results of studies on the distribution of invasive alien vascular plant species occurring in semi-natural and natural habitats in NW Poland

    Directory of Open Access Journals (Sweden)

    Popiela Agnieszka

    2015-03-01

    Full Text Available In Western Pomerania, as in other areas of Europe, alien species play an increasingly important role. In particular, invasive plants tend to spread rapidly and in large numbers which may reduce diversity of native species, leading to the phenomenon of “trivialisation of flora”, and transform ecosystems. The list of invasive species (32 taxa includes alien species occurring throughout Western Pomerania, and penetrating natural or semi-natural habitats. The second group consists of potentially invasive species (23 taxa, i.e. those distributed across the area under study and tending to increase the number of their localities in semi-natural and natural habitats, taxa invasive only locally, as well as species with missing data, which does not currently allow including them into the first group. Invasive weeds, as well as some epecophytes and archaeophytes occurring only on anthropogenic sites and tending to spread, were not taken into account. Among hemiagriophytes, the most common and troublesome ones are: Conyza canadensis, Erigeron annuus, Lolium multiflorum, Lupinus polyphyllus, Solidago canadensis, S. gigantea. Among holoagriophytes, i.e. the taxa which received the highest naturalisation status, very expansive species, successful in land colonisation, like Acer negundo, Bidens frondosa, B. connata, Clematis vitalba, Elodea canadensis, Epilobium ciliatum, Heracleum sosnowskyi, Impatiens glandulifera, I. parviflora, Padus serotina, Quercus rubra and Robinia pseudoacacia, should be given particular attention. Among the invasive and potentially invasive species, most taxa penetrate plant communities of the Artemisietea and Molinio-Arrhenatheretea class, followed by Querco-Fagetea, Vaccinio-Piceetea, Stellarietea mediae, Salicetea purpurae and Koelerio-Corynophoretea. The number of invasive species is twice as high when compared to the situation of these species in Poland; on the contrary, the number of species inhabiting anthropogenic, semi

  20. Streamflow and Soil Moisture of Agroforestry and Grass Watersheds in Hilly Area

    Institute of Scientific and Technical Information of China (English)

    LIU Gang-Cai; TIAN Guang-Long; SHU Dong-Cai; LIN San-Yi; LIU Shu-Zhen

    2004-01-01

    A study was conducted in a hilly area of Sichuan Province,Southwestern China, to compare the streamflow and soil moisture in two upland watersheds with different land use patterns. One was an agroforestry watershed, which consisted mainly of trees with alder (Alnus cremastogyne Burkill) and cypress (Cupressus funebris Endl.) planted in belts or strips with a coverage of about 46%, and the other was a grassland primarily composed of lalang grass (Imperata cylindrica var. major (Nees) C. E. Hubb.), filamentary clematis (Clematis filamentosa Dunn) and common eulaliopsis (Eulaliopsis binata (Retz.) C. E. Hubb) with a coverage of about 44%. Streamflow measurement with a hydrograph established at the watershed outlet showed that the average annual streamflow per 100 mm rainfall from 1983 to 1992 was 0.36 and 1.08 L s-1 km-2 for the agroforestry watershed and the grass watershed, respectively. This showed that the streamflow of the agroforestry watershed was reduced by 67% when compared to that of the grass watershed. The peak average monthly streamflow in the agroforestry watershed was over 5 times lower than that of the grass watershed and lagged by one month. In addition, the peak streamflow during a typical rainfall event of 38.3 mm in August 1986 was 37% lower in the agroforestry watershed than in the grass watershed. Results of the moisture contents of the soil samples from 3 slope locations (upper, middle and lower slopes) indicated that the agroforestry watershed maintained generally higher soil moisture contents than the grass watershed within 0-20 and 20-80 cm soil depths for the upper slope, especially for the period from May through July. For the other (middle and lower) slopes, soil moisture contents within 20-80 cm depth in the agroforestry watershed was generally lower than those in the grass watershed, particularly in September, revealing that water consumption by trees took place mainly below the plow layer. Therefore, agroforestry land use types might

  1. Do the Golden-winged Warbler and Blue-winged Warbler Exhibit Species-specific Differences in their Breeding Habitat Use?

    Directory of Open Access Journals (Sweden)

    David S. Maehr

    2010-12-01

    Full Text Available We compared habitat features of Golden-winged Warbler (Vermivora chrysoptera territories in the presence and absence of the Blue-winged Warbler (V. cyanoptera on reclaimed coal mines in southeastern Kentucky, USA. Our objective was to determine whether there are species specific differences in habitat that can be manipulated to encourage population persistence of the Golden-winged Warbler. When compared with Blue-winged Warblers, Golden-winged Warblers established territories at higher elevations and with greater percentages of grass and canopy cover. Mean territory size (minimum convex polygon was 1.3 ha (se = 0.1 for Golden-winged Warbler in absence of Blue-winged Warbler, 1.7 ha (se = 0.3 for Golden-winged Warbler coexisting with Blue-winged Warbler, and 2.1 ha (se = 0.3 for Blue-winged Warbler. Territory overlap occurred within and between species (18 of n = 73 territories, 24.7%. All Golden-winged and Blue-winged Warblers established territories that included an edge between reclaimed mine land and mature forest, as opposed to establishing territories in open grassland/shrubland habitat. The mean distance territories extended from a forest edge was 28.0 m (se = 3.8 for Golden-winged Warbler in absence of Blue-winged Warbler, 44.7 m (se = 5.7 for Golden-winged Warbler coexisting with Blue-winged Warbler, and 33.1 m (se = 6.1 for Blue-winged Warbler. Neither territory size nor distances to forest edges differed significantly between Golden-winged Warbler in presence or absence of Blue-winged Warbler. According to Monte Carlo analyses, orchardgrass (Dactylis glomerata, green ash (Fraxinus pennsylvanica seedlings and saplings, and black locust (Robinia pseudoacacia saplings were indicative of sites with only Golden-winged Warblers. Sericea lespedeza, goldenrod (Solidago spp., clematis vine (Clematis spp., and blackberry (Rubus spp. were indicative of sites where both species occurred. Our findings complement recent genetic studies and add

  2. Caryological notes in some portuguese Ranunculaceae

    Directory of Open Access Journals (Sweden)

    Queirós, Margarida

    1990-05-01

    Full Text Available Chromosome numbers of fourteen portuguese laxa of Ranunculaceae are reported: Helleborus foetidus 20 = 32; Nigella damascena 2n = 12; N. gallica 2n = 12; Delphinium Pentagynum 2n = 16; D. Halteratum subsp. verdunense 2n = 16; Anemone palmeta 2n = 32; Clematis campaniflora 2n = 16; Ranunculus muricatus 2n = 48; R. repens 2n = 32; R. bulbosus subsp. aleae var. adscendens 2n =16; R. sceleratus 2n = 32; R. paludosus 2n = 32; R. nigrescens 2n = 16; Aquilegia vulgaris subsp. dichroa 2n = 14. The chromosome numbers are in accordance with previous results.

    Se estudia el número cromosómico de algunos táxones de Ranunculaceae portugueses: Helleborus foetidus 2n = 32; Nigella damascena 2n = 12; N. gallica 2n = 12; Delphinium Pentagynum 2n = 16; D. Halteratum subsp. verdunense 2n = 16; Anemone palmeta 2n = 32; Clematis campaniflora 2n = 16; Ranunculus muricatus 2n = 48; R. repens 2n = 32; R. bulbosus subsp. aleae var. adscendens 2n =16; R. sceleratus 2n = 32; R. paludosus 2n = 32; R. nigrescens 2n = 16; Aquilegia vulgaris subsp. dichroa 2n = 14. Estos recuentos coinciden con los obtenidos anteriormente por otros autores.

  3. Avaliação dos efeitos centrais dos florais de Bach em camundongos através de modelos farmacológicos específicos Evaluation of central effects of Bach Flowers Remedies in mice using specific pharmacological models

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    Márcia M. De-Souza

    2006-09-01

    Full Text Available Os Remédios Florais de Bach (RFB, constituem um método alternativo de tratamento usado largamente na terapêutica de várias patologias em muitos países do mundo. Os RFB são reconhecidos como tratamento natural pela OMS desde 1956. Embora o mecanismo de ação dos RFB ainda não tenha sido elucidado, eles vêm sendo indicados para o tratamento de várias doenças neuropsiquiátricas. O objetivo do presente trabalho foi detectar possíveis efeitos centrais dos RFB em modelos farmacológicos utilizados na pesquisa de substâncias com efeitos ansiolíticos, hipnóticos, antidepressivos e neurolépticos. Para tanto, camundongos receberam um tratamento agudo via oral (0,45 mL 1 hora antes dos testes. Os resultados mostraram que os florais Gorse e, em conjunto, White chestnut, Agrymony e Vervain exibiram perfis antidepressivo e hipnótico, respectivamente. No modelo de ansiedade foi detectado efeito ansiolítico do floral Agrymony. Entretanto, não foram observados efeitos neurolépticos do floral Clematis. Os resultados nos levam a sugerir que os efeitos centrais dos florais avaliados podem ser parcialmente detectados através de modelos farmacológicos utilizados na pesquisa de agentes psicotrópicos.The Bach Flowers Remedies (BFR's are worldwide used as an alternative therapeutical approach for several pathologies, being considered by WHO as natural therapy since 1956. Despite the unknown mechanism of action, the BFR's have been widely used on treatment of several neuropsychiatry diseases. Based on pharmacological models used to detect ansiolitic, antidepressant, hypnotic and neuroleptyc effects of different substances, the aim of this work was to evaluate possible central effects of the BFR's. For this purpose, albino mice received BFR's treatment (0.45 mL by oral route 1 hour prior to each test. The results revealed that the Gorse flower alone and a mix of White chestnut, Agrymony and Vervain showed antidepressant and hypnotic effects

  4. In situ biomonitoring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life plants: Demonstration of the efficacy of emission controls in an eastern European city

    International Nuclear Information System (INIS)

    Aim of the study was to monitor changes of genotoxic activity of urban air caused by an incinerator and a petrochemical plant in Tradescantia micronucleus (Trad-MCN) and pollen fertility assays with wild plants (Chelidonium majus, Clematis vitalba, Cichorium intybus, Linaria vulgaris, Robinia pseudoacacia). While in the first sampling period (1997-2000) significantly (on average 80%) more MN were found at the polluted site in comparison to controls from a rural area, no significant effects were observed during a later period (between 2003 and 2005). A similar pattern was observed in the pollen abortion assays in which the most pronounced effects were found in chicory and false acacia. The differences of the results obtained in the two periods can be explained by a substantial reduction of air pollution by use of new technologies. In particular the decrease of SO2 emissions may account for the effects seen in the present study. - Air pollution caused by industrial emissions induced micronuclei in Tradescantia and increased pollen abortion in wild plant species

  5. In situ biomonitoring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life plants: Demonstration of the efficacy of emission controls in an eastern European city

    Energy Technology Data Exchange (ETDEWEB)

    Misik, Miroslav [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Micieta, Karol [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Solenska, Martina [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Misikova, Katarina [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Pisarcikova, Helena [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Knasmueller, Siegfried [Institute of Cancer Research, Department of Inner Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna (Austria)]. E-mail: siegfried.knasmueller@meduniwien.ac.at

    2007-01-15

    Aim of the study was to monitor changes of genotoxic activity of urban air caused by an incinerator and a petrochemical plant in Tradescantia micronucleus (Trad-MCN) and pollen fertility assays with wild plants (Chelidonium majus, Clematis vitalba, Cichorium intybus, Linaria vulgaris, Robinia pseudoacacia). While in the first sampling period (1997-2000) significantly (on average 80%) more MN were found at the polluted site in comparison to controls from a rural area, no significant effects were observed during a later period (between 2003 and 2005). A similar pattern was observed in the pollen abortion assays in which the most pronounced effects were found in chicory and false acacia. The differences of the results obtained in the two periods can be explained by a substantial reduction of air pollution by use of new technologies. In particular the decrease of SO{sub 2} emissions may account for the effects seen in the present study. - Air pollution caused by industrial emissions induced micronuclei in Tradescantia and increased pollen abortion in wild plant species.

  6. Synthesis and bioactivity evaluation of novel arylimines containing a 3-aminoethyl-2-[(p-trifluoromethoxy)anilino]-4(3H)-quinazolinone moiety.

    Science.gov (United States)

    Wang, Xiang; Li, Pei; Li, Zhining; Yin, Juan; He, Ming; Xue, Wei; Chen, Zhiwei; Song, Baoan

    2013-10-01

    Twenty-seven novel (E)-3-[2-arylideneaminoethyl]-2-[4-(trifluoromethoxy)anilino]-4(3H)-quinazolinone derivatives were synthesized by reacting various aromatic aldehydes with intermediate 6. The target compounds were characterized by (1)H NMR, (3)C NMR, IR, and elemental analysis. Bioassay results revealed that some of the compounds have strong antifungal activities against six fungi ( Gibberella zeae , Fusarium oxysporum , Clematis mandshurica , Paralepetopsis sasakii , Phytophthora infestans , and Sclerotinia sclerotiorum ) and three bacteria ( Xanthomonas oryzae , tomato bacterial wilt, and tobacco bacterial wilt). Notably, these compounds exhibited the highest activity against tomato bacterial wilt and X. oryzae, with 50% effective concentration (EC50) values ranging from 45.96 to 93.31 μg/mL and from 20.09 to 21.33 μg/mL, respectively, which are superior to those of the commercial antibacterial agents thiodiazole-copper (99.80 μg/mL) and bismerthiazol (92.61 μg/mL). These results indicate that novel arylimine derivatives containing the 4(3H)-quinazolinone moiety can effectively control tobacco bacterial wilt, tomato bacterial wilt, and X. oryzae. Evaluation of their bactericidal properties in field studies as well as the mechanisms underlying their enhanced antibacterial activity should be interesting topics for future investigations. PMID:24028303

  7. 几种乡土地被植物的耐荫性评价及应用研完

    Institute of Scientific and Technical Information of China (English)

    刘东焕; 赵世伟; 张佐双

    2008-01-01

    选取10种乡土地被植物为材料.并进行引种实验,对其耐荫性进行评价。结果表明。可把10种地被植物分为三组:(1)耐荫性较强的四种:红早莲(Hypericum ascyron)、大叶铁线莲(Clematis heracleifofia).蔓茎葵(Callirhoe involucrata)和杭子哨(Campylotropis macrocarpa)(2)耐半荫的四种:‘香铃'玉簪(Hosta’Honey Bells’)。狭叶玉簪(H.lancifolla)和‘像风信子’玉簪(H.Hyacinthina’)和扁叶刺芹(Eryngium planum)(3)耐荫性较弱的两种.秃玛丽’萱草(Hemerocallisx‘Mary Todd')和‘节日愉快’萱草(H.X‘Holiday Delight’)。并对其配置应用进行了探讨。

  8. Analysis of flavonoids by capillary zone electrophoresis with electrokinetic supercharging.

    Science.gov (United States)

    Zhong, Hao; Yao, Qingqiang; Breadmore, Michael C; Li, Yumei; Lu, Yuanqi

    2011-11-01

    On-line concentration via Electrokinetic Supercharging (EKS) was used to enhance the sensitivity of the capillary electrophoretic separation of the four flavonoids naringenin, hesperetin, naringin and hesperidin. Separation conditions, including the background electrolyte pH and concentration, the length and choice of terminator and the electrokinetic injection time were optimized. The optimum conditions were: a background electrolyte of 30 mM sodium tetraborate (pH 9.5) containing 5% (v/v) of methanol, electrokinetic injection of the sample (130 s, -10 kV) followed by hydrodynamic injecting of 100 mM 2-(cyclohexylamino)ethanesulfonic acid (CHES) (17 s, 0.5 psi) as terminator, and separation with -20 kV. Under these conditions the four flavonoids could be separated with a sample-to-sample time of 15 min and detection limits from 2.0 to 6.8 ng mL(-1). When compared to a conventional hydrodynamic injection the sensitivity was enhanced between 824 and 1515 times which is 7.6-16 times higher than other CE methods for the on-line concentration of flavonoids. The applicability of the developed method was demonstrated by the detection of the four flavonoids in an aqueous extract of Clematis hexapetala pall. PMID:21949941

  9. Screening of radical scavenging activity and polyphenol content of Bulgarian plant species

    Directory of Open Access Journals (Sweden)

    Milena Nikolova

    2011-01-01

    Full Text Available Background: Discovery of new plant species with antioxidant properties is a priority of many research teams. Most of the species included in this study are unstudied for antioxidant properties, but they are taxonomically related to reference plants with well-documented antioxidant activity. Materials and Methods: Free radical scavenging activity of plant extracts was evaluated using a 1,1-diphenyl-2-picrylhydrazyl (DPPH assay. An aluminum chloride colorimetric method was used for flavonoid determination. The amount of phenolic compounds in the extracts was estimated by using the Folin-Ciocalteu reagent. Results: As a result of screening, it was found that the significant antioxidant properties possess several unstudied until now plant species (Veronica bellidioides L., V. kellereri Deg. et Urm, V. vindobonensis (M. Fisher M. Fisher, V. beccabunga L., V. rhodopaea L., V. austriaca (Velen. Degen., Clinopodium vulgare L., Stachys recta L., Clematis vitalba L., and Xeranthemum annum L.. The antioxidant potential of the new species is comparable to that of reference medicinal plants. Conclusions: The existing data presented here provide new information for antioxidant potential of plant species that have not been traditionally used as medicinal plants.

  10. Ethnoveterinary medicine in the Arribes del Duero, western Spain.

    Science.gov (United States)

    González, José A; García-Barriuso, Mónica; Amich, Francisco

    2011-06-01

    Currently, traditional ethnoveterinary practices are rare in Europe and the plants used previously have been replaced by the modern drugs used by national veterinary services. However, in some rural areas of the Mediterranean basin these traditional practices persist. Here we analyze the plant resources still used, or that have been used up until recently, for the treatment of the health and comfort of animals in a region in the central-western part of the Iberian Peninsula, the Arribes del Duero. We document the use of 84 species, belonging to 39 families (with a total of 2243 use-reports), and 62 herbal remedies based on the use of a single plant species (43) and cited by at least three independent informants. The veterinary use of the Ranunculaceae Clematis campaniflora is reported for the first time. We also identify several plants used as bedding or fodder for livestock and discuss the relevance of some toxic plants and some "magical-curative" aspects reported in the territory. PMID:21461643

  11. Development and validation of a chromatographic method for determining Clematichinenoside AR and related impurities

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    Zhou Yang

    2012-12-01

    Full Text Available Abstract Background Clematichinenoside AR is a promising lead compound for the treatment of rheumatoid arthritis. A systematic research for the related impurities in AR bulk samples is still lacking. For the safe use of this natural product in future clinical practice, the structure and content of each constituent, including the main ingredient as well as the impurities in AR bulk sample must be characterized in detail. Results A simple and stability indicating RP-HPLC method was developed and validated for determining the purity of clematichinenoside AR (AR, a natural product from the roots of Clematis manshurica Rupr. (Ranunculaceae with the potential of treating rheumatoid arthritis. Five impurities were characterized, and impurity 2 (Clematomandshurica saponin F is a new triterpenoid saponin isolated from this product. Optimum separation for clematichinenoside AR and five related impurities was carried out on an Agilent octadecylsilane bonded silica gel column (TC-C18, 4.6 mm ×150 mm, 5 μm using a gradient HPLC method. The validation results showed good sensitivity, specificity, linearity(r2>0.9992 precision(RSD Conclusion Five related impurities of clematichinenoside AR were characterized, including a new triterpenoid saponins firstly found in clematichinenoside AR bulk samples. In the simple chromatographic method for determining clematichinenoside AR and its related impurities in bulk samples, the correction factor was better for the quality control in the relative stable concentrations.

  12. [Flower species identification and coverage estimation based on hyperspectral remote sensing data in Hulunbeier grassland].

    Science.gov (United States)

    Gai, Ying-Ying; Fan, Wen-Jie; Xu, Xi-Ru; Yan, Bin-Yan; Wang, Huan-Jiong; Liu, Yuan

    2011-10-01

    Monitoring grassland species and area real-timely and accurately is of great significance in species diversity research, as well as in sustainable development of ecosystem. Flowers have their own unique spectral characteristics. Compared with the nutrient stage, species are more easily identified by florescence. So, florescence is a critical period for identification. In the present paper, spectral differences among such flowers as Galium verum Linn., Hemerocallis citrina Baroni, Serratula centauroides Linn., Clematis hexapetala Pall., Lilium concolor var. pulchellum, Lilium pumilum and Artemisia frigida Willd. Sp. Pl. were found, along with identification methods, by analyzing canopies spectra and parametrizing characteristics. Verification results showed that when the coverage of flowers was greater than 10%, the accuracy of identification methods would be higher than 90%. On this basis, linear unmixing model was adopted to calculate the area of flowers in quadrates. Results showed that linear unmixing model was an effective method for estimating the coverage of flowers in grassland because the accuracy was about 4%. PMID:22250555

  13. The anticancer effect and mechanism of α-hederin on breast cancer cells.

    Science.gov (United States)

    Cheng, Lin; Xia, Tian-Song; Wang, Yi-Fen; Zhou, Wenbin; Liang, Xiu-Qing; Xue, Jin-Qiu; Shi, Liang; Wang, Ying; Ding, Qiang; Wang, Minhai

    2014-08-01

    Natural plant products occupy a very important position in the area of cancer chemotherapy. Many triterpenoid saponins have been proved as potential agents for chemoprevention and therapy of breast cancer. α-hederin, a monodesmosidic triterpenoid saponin distributed in Hedera or Nigella species, displays many biological activities. It is increasingly investigated for its promising anticancer potential since it has been shown to have cytotoxicity against several types of cancer cells. However, studies of α-hederin on breast cancer are limited, most of which focus on biological activity, while the mechanisms have not been widely reported yet. Previously, we purified and identified α-hederin from Clematis ganpiniana, a herb used in traditional Chinese medicine with antitumor action. In the present study, α-hederin showed strong inhibitory activity on the growth of breast cancer cells and induced apoptosis in these cells. α-hederin induced depolarization of mitochondrial membrane potential which released Apaf-1 and cytochrome c from the intermembrane space into the cytosol, where they promoted caspase-3 and caspase-9 activation. This is the first report on the growth inhibition and pro-apoptotic effects of α-hederin on breast cancer cells and the relative apoptosis pathways. It implied that triterpenoid saponin α-hederin could be a promising candidate for chemotherapy of breast cancer. PMID:24842044

  14. Ozone air pollution and foliar injury development on native plants of Switzerland

    Energy Technology Data Exchange (ETDEWEB)

    Novak, Kristopher; Skelly, John M.; Schaub, Marcus; Kraeuchi, Norbert; Hug, Christian; Landolt, Werner; Bleuler, Peter

    2003-09-01

    Visible ozone-induced foliar injury on native forest species of Switzerland was identified and confirmed under ambient OTC-conditions and related to the current European AOT40 standard. - The objectives of this study were to examine the foliar sensitivity to ozone exposure of 12 tree, shrub, and herbaceous species native to southern Switzerland and determine the seasonal cumulative ozone exposures required to induce visible foliar injury. The study was conducted from the beginning of May through the end of August during 2000 and 2001 using an open-top chamber research facility located within the Lattecaldo Cantonal Forest Nursery in Canton Ticino, southern Switzerland (600 m asl). Plants were examined daily and dates of initial foliar injury were recorded in order to determine the cumulative AOT40 ppb h ozone exposure required to cause visible foliar injury. Plant responses to ozone varied significantly among species; 11 species exhibited visible symptoms typical of exposures to ambient ozone. The symptomatic species (from most to least sensitive) were Populus nigra, Viburnum lantana, Salix alba, Crataegus monogyna, Viburnum opulus, Tilia platyphyllos, Cornus alba, Prunus avium, Fraxinus excelsior, Ribes alpinum, and Tilia cordata; Clematis spp. did not show foliar symptoms. Of the 11 symptomatic species, five showed initial injury below the critical level AOT40 10 ppmh O{sub 3} in the 2001 season.

  15. (7R,8S)-Dehydrodiconiferyl Alcohol Suppresses Lipopolysaccharide-Induced Inflammatory Responses in BV2 Microglia by Inhibiting MAPK Signaling.

    Science.gov (United States)

    Liu, Si-Yu; Xu, Peng; Luo, Xiao-Ling; Hu, Jin-Feng; Liu, Xin-Hua

    2016-07-01

    (7R,8S)-Dehydrodiconiferyl alcohol (DDA), a lignan isolated from the dried stems of Clematis armandii, has been found to exert potential anti-inflammatory activity in vitro. In the present study, we investigated the effects and possible mechanisms of DDA on lipopolysaccharide (LPS)-mediated inflammatory response in murine BV2 microglia. Our results revealed that non-toxic concentrations (6.25-25 μM) of DDA markedly suppressed LPS-induced production of nitric oxide, expression of inducible nitric oxide synthase and cyclooxygenase-2, and release of inflammatory factors, including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in a concentration dependent manner. In addition, DDA time- and concentration-dependently attenuated LPS-induced phosphorylation of c-Jun N-terminal kinase 1/2 (JNK), but not protein kinase B, p38, or extracellular signal-regulated kinase 1/2. Moreover, DDA significantly suppress LPS-mediated nuclear factor-κB (NF-κB) activation by inhibiting phosphorylation and nuclear translocation of NF-κB p65. Collectively, our results demonstrated that DDA inhibited LPS-stimulated inflammatory response in BV2 cell, at least in part, through inhibition of NF-κB activation and modulation of JNK signaling. PMID:26961887

  16. A cross-cultural study: anti-inflammatory activity of Australian and Chinese plants.

    Science.gov (United States)

    Li, Rachel W; Myers, Stephen P; Leach, David N; Lin, G David; Leach, Greg

    2003-03-01

    In this study, in vitro inhibitory effects of 33 ethanol extracts obtained from 24 plant species (representing 11 different families) on cyclooxygenase-1 (COX-1) were evaluated. The plant materials selected for this study have been used in aboriginal medicine in Australia and traditional medicine in China for the treatment of various diseases that are considered as inflammation in nature, e.g. asthma, arthritis, rheumatism, fever, edema, infections, snakebite and related inflammatory diseases. All of the selected plants, with one exception, showed inhibitory activity against COX-1, which supports their traditional uses. The most potent COX-1 inhibition were observed from the extracts of Acacia ancistrocarpa leaves (IC(50)=23 microg/ml). Ficus racemosa bark, Clematis pickeringii stem, Acacia adsurgens leaves, Tinospora smilacina stem and Morinda citrifolia fruit powder exhibited inhibition of COX-1 with the IC(50) of 100, 141, 144, 158 and 163 microg/ml, respectively. Aspirin and indomethacin used as the reference COX-1 inhibitors in this study inhibited COX-1 with IC(50) of 241 and 1.2 microg/ml, respectively. The findings of this study may explain at least in part why these plants have been traditionally used for the treatment of inflammatory conditions in Australian aboriginal medicine and traditional Chinese medicine. PMID:12576199

  17. Inhibition of key enzymes linked to obesity by preparations from Mediterranean dietary plants: effects on α-amylase and pancreatic lipase activities.

    Science.gov (United States)

    2013-12-01

    One of the most important strategy in the treatment of obesity includes the development of nutrient digestion and absorption inhibitors. Inhibition of digestive enzymes is one of the most widely studied mechanisms used to determine the potential efficacy of natural products as hypolipidemic and hypoglycaemic agents. In vitro studies here reported were performed to evaluate the inhibitory activity of five species(as hydroalcoholic extracts) of edible plants from Calabria region (Italy) on amylase and lipase by monitoring the hydrolysis of p-NPC and the hydrolysis of glycoside bonds indigestible carbohydrate foods. The formulation obtained from Clematis vitalba L. exhibited the strongest inhibitory effect on pancreatic lipase (IC50=0.99 mg/ml) and on α-amylase(IC50=31.52 μg/ml). In order to explore metabolome production HPTLC analysis of the extracts was performed, revealing the predominance of (±)-catechin, caffeic acid and chlorogenic acid in C. vital ba formulation at concentration of 23.18±3.14,13.63±0.65 and 18.88±0.76 mg/g, respectively. GC/MS analysis was used to identify fatty acids and terpene composition. PMID:24122547

  18. Effect and mechanism of AR-6 in experimental rheumatoid arthritis.

    Science.gov (United States)

    Sun, Shi-xue; Li, Yun-man; Fang, Wei-rong; Cheng, Peng; Liu, Lifang; Li, Fengwen

    2010-06-01

    The root of Clematis chinensis Osbeck has been used widely in rheumatoid arthritis in Chinese traditional medicine and AR-6 is a triterpene saponin isolated from it. In this present study, we investigated in vivo effects of oral AR-6 in chronic rat adjuvant-induced arthritis (AA) and in vitro effect in macrophage and synoviocytes cells. Arthritic scores and serum inflammatory mediators were evaluated 19 days after AA induction by endermic injection of Freund's complete adjuvant in Sprague-Dawley(S-D) rats. Oral administration of AR-6 to arthritic rats resulted in a clear decrease of clinical signs compared to untreated controls. The synoviocyte and macrophage response ex vivo were then analyzed. Anti-arthritic effects of AR-6 correlated with significant decrease of NO and TNF-alpha produced by peritoneal macrophages, ex vivo and in vitro. AR-6 also significant decreased the proliferation of synoviocyte. These data indicate that AR-6 is a potential anti-inflammatory therapeutic and preventive agent. PMID:19842015

  19. Ozone air pollution and foliar injury development on native plants of Switzerland

    International Nuclear Information System (INIS)

    Visible ozone-induced foliar injury on native forest species of Switzerland was identified and confirmed under ambient OTC-conditions and related to the current European AOT40 standard. - The objectives of this study were to examine the foliar sensitivity to ozone exposure of 12 tree, shrub, and herbaceous species native to southern Switzerland and determine the seasonal cumulative ozone exposures required to induce visible foliar injury. The study was conducted from the beginning of May through the end of August during 2000 and 2001 using an open-top chamber research facility located within the Lattecaldo Cantonal Forest Nursery in Canton Ticino, southern Switzerland (600 m asl). Plants were examined daily and dates of initial foliar injury were recorded in order to determine the cumulative AOT40 ppb h ozone exposure required to cause visible foliar injury. Plant responses to ozone varied significantly among species; 11 species exhibited visible symptoms typical of exposures to ambient ozone. The symptomatic species (from most to least sensitive) were Populus nigra, Viburnum lantana, Salix alba, Crataegus monogyna, Viburnum opulus, Tilia platyphyllos, Cornus alba, Prunus avium, Fraxinus excelsior, Ribes alpinum, and Tilia cordata; Clematis spp. did not show foliar symptoms. Of the 11 symptomatic species, five showed initial injury below the critical level AOT40 10 ppmh O3 in the 2001 season

  20. Ozone air pollution and foliar injury development on native plants of Switzerland.

    Science.gov (United States)

    Novak, Kristopher; Skelly, John M; Schaub, Marcus; Kräuchi, Norbert; Hug, Christian; Landolt, Werner; Bleuler, Peter

    2003-01-01

    The objectives of this study were to examine the foliar sensitivity to ozone exposure of 12 tree, shrub, and herbaceous species native to southern Switzerland and determine the seasonal cumulative ozone exposures required to induce visible foliar injury. The study was conducted from the beginning of May through the end of August during 2000 and 2001 using an open-top chamber research facility located within the Lattecaldo Cantonal Forest Nursery in Canton Ticino, southern Switzerland (600 m asl). Plants were examined daily and dates of initial foliar injury were recorded in order to determine the cumulative AOT40 ppb h ozone exposure required to cause visible foliar injury. Plant responses to ozone varied significantly among species; 11 species exhibited visible symptoms typical of exposures to ambient ozone. The symptomatic species (from most to least sensitive) were Populus nigra, Viburnum lantana, Salix alba, Crataegus monogyna, Viburnum opulus, Tilia platyphyllos, Cornus alba, Prunus avium, Fraxinus excelsior, Ribes alpinum, and Tilia cordata; Clematis spp. did not show foliar symptoms. Of the 11 symptomatic species, five showed initial injury below the critical level AOT40 10 ppmh O3 in the 2001 season. PMID:12804826

  1. Antisporulant Activity of Watery Extracts of Plants against Sclerospora graminicola Causing Downy Mildew Disease of Pearl Millet

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    S. A. Deepak

    2007-01-01

    Full Text Available Watery extracts of forty plant species commonly growing in across India have been screened for antisporulant activity against Sclerospora graminicola (Sacc. Schroet., the causative agent of pearl millet downy mildew. The collection represented 38 genera of 30 families. The extracts of thirteen species did not show any effect, whereas the activity of extracts of Allium sativum, Clematis gouriana, Evolvulus alsinoides, Mimusops elengi, Parthenium hysterophorus, Piper nigrum and Tagetes erecta were commensurable to that of marketed botanical fungicides and Mikal 70 wp. The crude extracts of 12 species (Agave americana, Aloe vera, Artemisia parviflora, Citrus limon, Citrus sinensis, Eucalyptus globosus, Euphorbia hirta, Leucas aspera, Murraya koenigi, Ocimum sanctum, Santalum album and Zingiber offinale completely inhibited the zoosprorangium formation while in the case of remaining 8 plants the crude extracts reduced only partially the sporulation. The antisporulant activity of commercialised Azadirachta preparation (Nutri-Neem was more pronounced than that of Reynutria based one (Milsana and Sabadilla (veratrin, however, these botanical preparations held off synthetic fungicides and the most active watery extracts.

  2. In vitro evaluering van die effektiwiteit van vyf plante wat tradisioneel teen seksueel oordraagbare siektes gebruik word

    Directory of Open Access Journals (Sweden)

    H. Swart

    2002-09-01

    Full Text Available Tot soveel as 60% van die Suid-Afrikaanse bevolking raadpleeg tradisionele genesers vir siektetoestande, insluitende seksueel oordraagbare siektes (SOS. Medisinale plante speel ’n belangrike rol in die behandeling van SOS deur tradisionele genesers, maar sonder enige wetenskaplike bewyse van effektiwiteit. Vir hierdie studie is 5 plante vanuit die literatuur gekies op grond van hul gebruik deur tradisionele genesers vir die behandeling van SOS. Siftingstoetse vir die bepaling van aktiwiteit teen bakterieë en teen SOS is op die plante uitgevoer. Die plante was Clematis brachiata, Elephantorrhiza elephantina, Lepidium bonariense, Ranunculus multifidus en Typha capensis. Siftingstoetse is uitgevoer met die organismes Candida albicans, Neisseria gonorrhoeae en Haemophilus ducreyi wat met SOS geassosieer word, terwyl Bacillus subtilus, Micrococcus luteus, Staphylococcus aureus, Enterobacter sp., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa en Aspergillus niger ook in die siftingstoetse ingesluit is. Slegs Lepidium bonariense het aktiwiteit teen die gebruikte organismes getoon. Dit is dus duidelik dat daar ’n behoefte bestaan om die effektiwiteit van medisinale plante wetenskaplik te bepaal en te kommunikeer.

  3. 间歇期及慢性期痛风中医方药的文献研究%Literature Research of Traditional Chinese Medical Prescriptions of Gout in Intermission and Chronic Phase

    Institute of Scientific and Technical Information of China (English)

    李海昌; 温成平; 谢志军; 韩春雯; 汪梅姣

    2013-01-01

    目的:探求痛风间歇期及慢性期的方药特点,为中医临床治疗奠定基础.方法:采用计算机检索和人工检索相结合的方法,对1997年1月-2011年6月国内期刊中相关文献进行回顾性总结.结果:检索出符合要求的文献85[1-82]篇,共涉及痛风间歇期、慢性期及慢性关节炎期患者累计4791例,主要中药(前15味)使用频率结果分别是:牛膝(36.11%)、桑寄生(31.48%)、薏苡仁(30.56%)、土茯苓(26.85%)、苍术(25.93%)、白术(25.93%)、白芥子(25.00%)、当归(24.07%)、黄柏(23.15%)、黄芪(22.22%)、防己(22.22%)、草薢(20.37%)、威灵仙(20.37%)、赤芍(18.52%)、秦艽(16.67%).结论:牛膝、桑寄生、苍白术、薏苡仁及黄芪健脾益肾,泄浊祛湿治病之本;当归、牛膝、苍白术、赤芍、黄柏、白芥子及威灵仙活血化瘀通络,燥湿化痰通痹治病之标.%Objective: To provide a basis for clinical treatment of traditional Chinese medicine ( TCM) by seeking the TCM prescriptions of gout in intermission and chronic phase. Methods: Domestic relevant literatures were summarized retrospectively based on the interaction by computer search and artificial retrieval from January 1997 to June 2011. Results-. Totally 85 literatures were retrieved to meet the requirements, including 4791 cases of intermission, chronic phase and chronic gouty arthritis. The distributions of major TCM prescriptions (the top 15)were achyranthes root(36. 11% ) ,mis-tletoe( 31. 48% ) , coix seed ( 30. 56% ), smilax Glabra ( 26. 85% ), atractylodes ( 25.93% ) , rhizoma atractylodis (25.93%) ,white mustard seed(25.00% ) ,angelica sinensis(24. 07% ) ,cortex phellodendri(23. 15%) ,radix astragali (22.22% ) ,tetrandra root(22.22%) ,yam rhizome(20. 37% ),clematis root(20. 37% ) ,red peony root(18.52%) ,gen-tiana macrophylla ( 16. 67% ). Conclusions :It is the fundamental treatment of gout by invigorating spleen and nourishing kidney therapy for reducing

  4. Evaluación in vivo de la actividad antimalárica de 25 plantas provenientes de una Reserva de Conservación Biológica de Costa Rica In vivo evaluation of the antimalarial activity of 25 plants from a Biological Conservation Reserve of Costa Rica

    Directory of Open Access Journals (Sweden)

    MISAEL CHINCHILLA-CARMONA

    2011-03-01

    Full Text Available Se realizó una evaluación in vivo de la actividad antimalárica de las hojas, flores, frutos, corteza y raíz de 25 plantas de la Reserva Biológica Alberto Manuel Brenes (REBAMB, situada en San Ramón, Alajuela, Costa Rica. Las plantas estudiadas fueron Aphelandra aurantiaca (Scheidw. Lindl., Aphelandra tridentata Hemsl. (Acanthaceae, Xanthosoma undipes (K. Koch & C.D. Bouché K. Koch. (Araceae, Iriartea deltoidea Ruiz & Pav. (Arecaceae, Neurolaena lobata (L. Cass. (Asteraceae, Lonchocarpus pentaphyllus (Poir. Kunth ex DC., Pterocarpus hayesii Hemsl., Senna papillosa (Britton & Rose H.S. Irwin & Barneby., Cinnamomum chavarrianum (Hammel Kosterm. (Fabaceae, Nectandra membranacea (Sw. Griseb., Persea povedae W.C. Burger. (Lauraceae, Hampea appendiculata (Donn. Sm. Standl. (Malvaceae, Guarea glabra Vahl., Ruagea glabra Triana & Planch. (Meliaceae, Psidium guajava L. (Myrtaceae, Bocconia frutescens L. (Papaveraceae, Piper friedrichsthalii C. DC. (Piperaceae, Clematis dioica L. (Ranunculaceae, Prunus annularis Koehne. (Rosaceae, Siparuna thecaphora (Poepp. & Endl. A. DC. (Siparunaceae, Solanum arboreum Dunal., Witheringia solanacea L'Hér. (Solanaceae, Ticodendron incognitum Gómez-Laur. & L.D. Gómez. (Ticodendraceae, Heliocarpus appendiculatus Turcz. (Tiliaceae y Myriocarpa longipes Liebm. (Urticaceae. Los extractos alcohólicos frescos y secos, fueron evaluados por su actividad inhibitoria de la parasitemia causada por Plasmodium berghei en ratones Swiss. Al realizar las prueba de CI50 las plantas en que esa actividad fue muy relevante fueron (en mg kg-1 de peso: 12 para la corteza de B. frutescens, 18 para la raíz de H. appendiculata, 14 para la raíz de I. deltoidea, 4 para el fruto inmaduro de M. longipes, 21 para la raíz de N. membranacea, 19 para las hojas tiernas de P. povedae y 16 para el fruto inmaduro de S. tecaphora. Los extractos frescos presentaron una mayor actividad antimalárica que los sometidos a desecación. Este estudio es

  5. Downregulation of NO and PGE2 in LPS-stimulated BV2 microglial cells by trans-isoferulic acid via suppression of PI3K/Akt-dependent NF-κB and activation of Nrf2-mediated HO-1.

    Science.gov (United States)

    Dilshara, Matharage Gayani; Lee, Kyoung-Tae; Jayasooriya, Rajapaksha Gedara Prasad Tharanga; Kang, Chang-Hee; Park, Sang Rul; Choi, Yung Hyun; Choi, Il-Whan; Hyun, Jin-Won; Chang, Weon-Young; Kim, Yeon-Su; Lee, Hak-Ju; Kim, Gi-Young

    2014-01-01

    Little is known about whether trans-isoferulic acid (TIA) regulates the production of lipopolysaccharide (LPS)-induced proinflammatory mediators. Therefore, we examined the effect of TIA isolated from Clematis mandshurica on LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in BV2 microglial cells. We found that TIA inhibited the production of LPS-induced NO and PGE2 without accompanying cytotoxicity in BV2 microglial cells. TIA also downregulated the expression levels of specific regulatory genes such as inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) by suppressing LPS-induced NF-κB activity via dephosphorylation of PI3K/Akt. In addition, we demonstrated that a specific NF-κB inhibitor PDTC and a selective PI3K/Akt inhibitor, LY294002 effectively attenuated the expression of LPS-stimulated iNOS and COX-2 mRNA, while LY294002 suppressed LPS-induced NF-κB activity, suggesting that TIA attenuates the expression of these proinflammatory genes by suppressing PI3K/Akt-mediated NF-κB activity. Our results showed that TIA suppressed NO and PGE2 production through the induction of nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent heme oxygenase-1 (HO-1). Taken together, our data indicate that TIA suppresses the production of proinflammatory mediators such as NO and PGE2, as well as their regulatory genes, in LPS-stimulated BV2 microglial cells, by inhibiting PI3K/Akt-dependent NF-κB activity and enhancing Nrf2-mediated HO-1 expression. PMID:24291391

  6. Disruption of the petal identity gene APETALA3-3 is highly correlated with loss of petals within the buttercup family (Ranunculaceae).

    Science.gov (United States)

    Zhang, Rui; Guo, Chunce; Zhang, Wengen; Wang, Peipei; Li, Lin; Duan, Xiaoshan; Du, Qinggao; Zhao, Liang; Shan, Hongyan; Hodges, Scott A; Kramer, Elena M; Ren, Yi; Kong, Hongzhi

    2013-03-26

    Absence of petals, or being apetalous, is usually one of the most important features that characterizes a group of flowering plants at high taxonomic ranks (i.e., family and above). The apetalous condition, however, appears to be the result of parallel or convergent evolution with unknown genetic causes. Here we show that within the buttercup family (Ranunculaceae), apetalous genera in at least seven different lineages were all derived from petalous ancestors, indicative of parallel petal losses. We also show that independent petal losses within this family were strongly associated with decreased or eliminated expression of a single floral organ identity gene, APETALA3-3 (AP3-3), apparently owing to species-specific molecular lesions. In an apetalous mutant of Nigella, insertion of a transposable element into the second intron has led to silencing of the gene and transformation of petals into sepals. In several naturally occurring apetalous genera, such as Thalictrum, Beesia, and Enemion, the gene has either been lost altogether or disrupted by deletions in coding or regulatory regions. In Clematis, a large genus in which petalous species evolved secondarily from apetalous ones, the gene exhibits hallmarks of a pseudogene. These results suggest that, as a petal identity gene, AP3-3 has been silenced or down-regulated by different mechanisms in different evolutionary lineages. This also suggests that petal identity did not evolve many times independently across the Ranunculaceae but was lost in numerous instances. The genetic mechanisms underlying the independent petal losses, however, may be complex, with disruption of AP3-3 being either cause or effect. PMID:23479615

  7. Retreating or standing: responses of forest species and steppe species to climate change in arid Eastern Central Asia.

    Directory of Open Access Journals (Sweden)

    Hong-Xiang Zhang

    Full Text Available BACKGROUND: The temperature in arid Eastern Central Asia is projected to increase in the future, accompanied by increased variability of precipitation. To investigate the impacts of climate change on plant species in this area, we selected two widespread species as candidates, Clematis sibirica and C. songorica, from montane coniferous forest and arid steppe habitats respectively. METHODOLOGY/PRINCIPAL FINDINGS: We employed a combined approach of molecular phylogeography and species distribution modelling (SDM to predict the future responses of these two species to climate change, utilizing evidence of responses from the past. Genetic data for C. sibirica shows a significant phylogeographical signal (N ST > F ST, P<0.05 and demographic contraction during the glacial-interglacial cycles in the Pleistocene. This forest species would likely experience range reduction, though without genetic loss, in the face of future climate change. In contrast, SDMs predict that C. songorica, a steppe species, should maintain a consistently stable potential distribution under the Last Glacial Maximum (LGM and the future climatic conditions referring to its existing potential distribution. Molecular results indicate that the presence of significant phylogeographical signal in this steppe species is rejected and this species contains a high level of genetic differentiation among populations in cpDNA, likely benefiting from stable habitats over a lengthy time period. CONCLUSIONS/SIGNIFICANCE: Evidence from the molecular phylogeography of these two species, the forest species is more sensitive to past climate changes than the steppe species. SDMs predict that the forest species will face the challenge of potential range contraction in the future more than the steppe species. This provides a perspective on ecological management in arid Eastern Central Asia, indicating that increased attention should be paid to montane forest species, due to their high sensitivity to

  8. Drug metabolism and pharmacokinetic diversity of ranunculaceae medicinal compounds.

    Science.gov (United States)

    Hao, Da-Cheng; Ge, Guang-Bo; Xiao, Pei-Gen; Wang, Ping; Yang, Ling

    2015-01-01

    The wide-reaching distributed angiosperm family Ranunculaceae has approximately 2200 species in around 60 genera. Chemical components of this family include several representative groups: benzylisoquinoline alkaloid (BIA), ranunculin, triterpenoid saponin and diterpene alkaloid, etc. Their extensive clinical utility has been validated by traditional uses of thousands of years and current evidence-based medicine studies. Drug metabolism and pharmacokinetic (DMPK) studies of plant-based natural products are an indispensable part of comprehensive medicinal plant exploration, which could facilitate conservation and sustainable utilization of Ranunculaceae pharmaceutical resources, as well as new chemical entity development with improved DMPK parameters. However, DMPK characteristics of Ranunculaceaederived medicinal compounds have not been summarized. Black cohosh (Cimicifuga) and goldenseal (Hydrastis) raise concerns of herbdrug interaction. DMPK studies of other Ranunculaceae genera, e.g., Nigella, Delphinium, Aconitum, Trollius, and Coptis, are also rapidly increasing and becoming more and more clinically relevant. In this contribution, we highlight the up-to-date awareness, as well as the challenges around the DMPK-related issues in optimization of drug development and clinical practice of Ranunculaceae compounds. Herb-herb interaction of Ranunculaceae herb-containing traditional Chinese medicine (TCM) formula could significantly influence the in vivo pharmacokinetic behavior of compounds thereof, which may partially explain the complicated therapeutic mechanism of TCM formula. Although progress has been made on revealing the absorption, distribution, metabolism, excretion and toxicity (ADME/T) of Ranunculaceae compounds, there is a lack of DMPK studies of traditional medicinal genera Aquilegia, Thalictrum and Clematis. Fluorescent probe compounds could be promising substrate, inhibitor and/or inducer in future DMPK studies of Ranunculaceae compounds. A better

  9. Anti-inflammatory mechanism of α-viniferin regulates lipopolysaccharide-induced release of proinflammatory mediators in BV2 microglial cells.

    Science.gov (United States)

    Dilshara, Matharage Gayani; Lee, Kyoung-Tae; Kim, Hee Ju; Lee, Hak-Ju; Choi, Yung Hyun; Lee, Chang-Min; Kim, Lark Kyun; Kim, Gi-Young

    2014-07-01

    α-Viniferin is an oligostilbene of trimeric resveratrol and has anticancer activity; however, the molecular mechanism underlying the anti-inflammatory effects of α-viniferin has not been completely elucidated thus far. Therefore, we determined the mechanism by which α-viniferin regulates lipopolysaccharide (LPS)-induced expression of proinflammatory mediators in BV2 microglial cells. Treatment with α-viniferin isolated from Clematis mandshurica decreased LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2). α-Viniferin also downregulated the LPS-induced expression of proinflammatory genes such as iNOS and COX-2 by suppressing the activity of nuclear factor kappa B (NF-κB) via dephosphorylation of Akt/PI3K. Treatment with a specific NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC), indirectly showed that NF-κB is a crucial transcription factor for expression of these genes in the early stage of inflammation. Additionally, our results indicated that α-viniferin suppresses NO and PGE2 production in the late stage of inflammation through induction of heme oxygenase-1 (HO-1) regulated by nuclear factor erythroid 2-related factor (Nrf2). Taken together, our data indicate that α-viniferin suppresses the expression of proinflammatory genes iNOS and COX-2 in the early stage of inflammation by inhibiting the Akt/PI3K-dependent NF-κB activation and inhibits the production of proinflammatory mediators NO and PGE2 in the late stage by stimulating Nrf2-mediated HO-1 signaling pathway in LPS-stimulated BV2 microglial cells. These results suggest that α-viniferin may be a potential candidate to regulate LPS-induced inflammation. PMID:24859013

  10. The apoptotic effect of D Rhamnose β-hederin, a novel oleanane-type triterpenoid saponin on breast cancer cells.

    Science.gov (United States)

    Cheng, Lin; Xia, Tian-Song; Wang, Yi-Fen; Zhou, Wenbin; Liang, Xiu-Qing; Xue, Jin-Qiu; Shi, Liang; Wang, Ying; Ding, Qiang

    2014-01-01

    There is growing interest in development of natural products as anti-cancer and chemopreventive agents. Many triterpenoids have been proved as potential agents for chemoprevention and therapy of breast cancer. Ginsenosides from ginseng, which mostly belong to dammarane-type triterpenoids, have gained great attention for their anti-breast cancer activity with diverse mechanisms. However, studies of other kinds of triterpenoid saponins on breast cancer are limited. Previously, we purified and identified a novel oleanane-type triterpene saponin named D Rhamnose β-hederin (DRβ-H) from Clematis ganpiniana, a Chinese traditional anti-tumor herb. In the present study, DRβ-H showed strong inhibitory activity on the growth of various breast cancer cells and induced apoptosis in these cells. DRβ-H inhibited PI3K/AKT and activated ERK signaling pathway. PI3K inhibitor LY294002 synergistically enhanced DRβ-H-induced apoptosis whereas MEK inhibitor U0126 reduced the apoptosis rate. Moreover, DRβ-H regulated the ratio of pro-apoptotic and anti-apoptotic Bcl-2 family proteins. Furthermore, DRβ-H induced depolarization of mitochondrial membrane potential which released Apaf-1 and Cytochrome C from the inter membrane space into the cytosol, where they promoted caspase-9 and caspase-3 activation. This is the first report on the pro-apoptotic effects of DRβ-H, a novel oleanane-type triterpenoid saponin, on breast cancer cells and its comprehensive apoptosis pathways. It implied that oleanane-type triterpenoid saponin DRβ-H could be a promising candidate for chemotherapy of breast cancer. PMID:24603880

  11. Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations.

    Science.gov (United States)

    Krstić, O; Cvrković, T; Mitrović, M; Toševski, I; Jović, J

    2016-06-01

    The European lantern fly Dictyophara europaea (Linnaeus, 1767), is a polyphagous dictyopharid planthopper of Auchenorrhyncha commonly found throughout the Palaearctic. Despite abundant data on its distribution range and reports on its role in the epidemiology of plant-pathogenic phytoplasmas (Flavescence dorée, FD-C), literature regarding the biology and host plants of this species is scarce. Therefore, the aims of our study were to investigate the seasonal occurrence, host plant associations, oviposition behaviour and immature stages of this widespread planthopper of economic importance. We performed a 3-year field study to observe the spatio-temporal distribution and feeding sources of D. europaea. The insects's reproductive strategy, nymphal molting and behaviour were observed under semi-field cage conditions. Measurement of the nymphal vertex length was used to determine the number of instars, and the combination of these data with body length, number of pronotal rows of sensory pits and body colour pattern enabled the discrimination of each instar. We provide data showing that D. europaea has five instars with one generation per year and that it overwinters in the egg stage. Furthermore, our study confirmed highly polyphagous feeding nature of D. europaea, for all instars and adults, as well as adult horizontal movement during the vegetation growing season to the temporarily preferred feeding plants where they aggregate during dry season. We found D. europaea adult aggregation in late summer on Clematis vitalba L. (Ranunculaceae), a reservoir plant of FD-C phytoplasma strain; however, this appears to be a consequence of forced migration due to drying of herbaceous vegetation rather than to a high preference of C. vitalba as a feeding plant. Detailed oviposition behaviour and a summary of the key discriminatory characteristics of the five instars are provided. Emphasis is placed on the economic importance of D. europaea because of its involvement in

  12. Potential chemoprevention of LPS-stimulated nitric oxide and prostaglandin E₂ production by α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranosyl-3-indolecarbonate in BV2 microglial cells through suppression of the ROS/PI3K/Akt/NF-κB pathway.

    Science.gov (United States)

    Dilshara, Matharage Gayani; Lee, Kyoung-Tae; Choi, Yung Hyun; Moon, Dong-Oh; Lee, Hak-Ju; Yun, Sung Gyu; Kim, Gi-Young

    2014-02-01

    α-l-Rhamnopyranosyl-(1→6)-β-d-glucopyranosyl-3-indolecarbonate (RG3I) is a chemical constituent isolated from the commonly used Asian traditional medicinal plant, Clematis mandshurica; however, no studies have been reported on its anti-inflammatory properties. In the present study, we found that RG3I attenuates the lipopolysaccharide (LPS)-induced DNA-binding activity of nuclear factor-κB (NF-κB) via the dephosphorylation of PI3K/Akt in BV2 microglial cells, leading to a suppression of nitric oxide (NO) and prostaglandin E2 (PGE2) production, along with that of their regulatory genes, inducible NO synthase (iNOS) and cyclooxygenase-2 (Cox-2). Further, the PI3K/Akt inhibitor, LY294002 diminished the expression of LPS-stimulated iNOS and COX-2 genes by suppressing NF-κB activity. Moreover, RG3I significantly inhibited LPS-induced reactive oxygen species (ROS) generation similar to the ROS inhibitors, N-acetylcysteine (NAC) and glutathione (GSH). Notably, NAC and GSH abolished the LPS-induced expression of iNOS and Cox-2 in BV2 microglial cells by inhibiting NF-κB activity. Taken together, our data indicate that RG3I suppresses the production of proinflammatory mediators such as NO and PGE2 as well as their regulatory genes in LPS-stimulated BV2 microglial cells by inhibiting the PI3K/Akt- and ROS-dependent NF-κB signaling pathway, suggesting that RG3I may be a good candidate to regulate LPS-induced inflammatory response. PMID:24486459

  13. An ethnoveterinary study of medicinal plants in treatment of diseases and syndromes of herd dog in southern regions of Ilam province, Iran.

    Science.gov (United States)

    Bahmani, Mahmoud; Eftekhari, Zohre

    2013-05-01

    This paper describes a selection of the ethnoveterinary medicines used for herd dogs in the southern regions of Ilam province, Iran. Traditional botanical medicine is the primary mode of healthcare for most of the rural population in Ilam province. In this study, a questionnaire was distributed among 45 residential areas in 22 rural zones of the southern areas of Ilam province. The objective of this study was the recognition of natural medicinal methods using medicinal plants, and the classification of ethnoveterinary applications and collection of domestic science. Twenty-two medicinal plants from 16 families were identified. The main application of these plants was for the detection and treatment of digestive disorders using Citrullus colocynthis, Aristolochia clematis, Scrophularia deserti, Quercus brantii, Ceracus microcarpa, Echium strigosa, Pistacia atlantica, and Pistacia khinjuk which have been applied using Euphurbia graminifolius, Peganum harmala, Salsola rigida, Artemisia herba-alba, Amygdalus arabica, jolbak of salt water, Peganum harmala L., and Nicotina tabacum for external and internal parasite disorders. S. deserti for ophthalmic disorders, and P. atlantica, P. khinjuk, and Q. brantii for respiratory disorders were applied. The present study confirmed the traditional medical effects of some plants and revealed the unique medical effects of other plants, which if recognized could be useful in the creation of new ideas and increasing knowledge for the modern pharmaceutical industry. Since very few clinical trials have been conducted on plants native to Ilam province, it is necessary that more research be conducted to ensure that labeled and standardized products are introduced for human consumption. PMID:23667351

  14. Species Structure of Plants in the Báb Forest Clearcuts

    Directory of Open Access Journals (Sweden)

    Pilková Ivana

    2014-12-01

    Full Text Available In the paper we have summarized the results of a research which was realized in the Báb forest (Veľký Báb, Nitra upland. The target of the research is the evaluation of species composition in the clearcuts in 2012. In the Báb forest, during spring records there were 80 and during summer records 102 taxa of taxons recorded. The woody plants of spring and summer reports were mainly represented by typical forest species. Moreover, these are woody plants of forest open parts and there are also two invasive woody Ailanthus altissima, Robinia pseudoacacia plants documented. During the summer reports, three new woody plants Clematis vitalba, Lonicera caprifolium, Ulmus minor appeared in the herb layer. Herbs are represented during the spring reports by typical spring ephemeroids, geophytes and forest herbs presenting the spring synusia. During the summer reports, ephemeroids are absent and there were new species, mainly Alliaria petiolata, Convallaria majalis, Lithospermum purpurocaeruleum, Melica nutans of forest herbs reported. In the clearcut areas also clearcut, synanthropic, mainly Cirsium vulgare, Lamium purpureum, Sambucus ebulus, Serratula tinctoria, Torilis japonica and invasive species Aster lanceolatus, A. novi-belgii agg., Erigeron annuus ssp. annuus, Impatiens parviflora occurred. Generally, we can state that the diversity of clearcut plant taxa is high. Taxa are represented by forest woody plants, woody plants of clearcuts, forest open parts and forest edges. Within clearcut herbs, there are typical forest species of oak-hornbeam forests represented, species of clearcuts and human-influenced posts and there are also invasive taxa found

  15. Clematichinenoside inhibits VCAM-1 and ICAM-1 expression in TNF-α-treated endothelial cells via NADPH oxidase-dependent IκB kinase/NF-κB pathway.

    Science.gov (United States)

    Yan, Simin; Zhang, Xu; Zheng, Haili; Hu, Danhong; Zhang, Yongtian; Guan, Qinghua; Liu, Lifang; Ding, Qilong; Li, Yunman

    2015-01-01

    Proinflammatory cytokine TNF-α-induced adhesion of leukocytes to endothelial cells plays a critical role in the early stage of atherosclerosis. Oxidative stress and redox-sensitive transcription factors are implicated in the process. Thus, compounds that mediate intracellular redox status and regulate transcription factors are of great therapeutic interest. Clematichinenoside (AR), a triterpene saponin isolated from the root of Clematis chinensis Osbeck, was previously demonstrated to have anti-inflammatory and antioxidative properties. However, little is known about the exact mechanism underlying these actions. Thus we performed a detailed study on its effect on leukocytes-endothelial cells adhesion with TNF-α-stimulated human umbilical vein endothelial cells (HUVECs) and cell-free systems. First, we found that AR reduced TNF-α-induced VCAM-1 and ICAM-1 expression and their promoter activity, inhibited translocation of p65 and phosphorylation of IκBα, suppressed IκB kinase-β (IKK-β) activity, lowered O2(∙-) and H2O2 levels, tackled p47(phox) translocation, and decreased NOX4 NADPH oxidase expression. Second, we showed that AR exhibited no direct free radical scavenging ability in cell-free systems at concentrations that were used in intact cells. Besides, AR had no direct effect on the activity of IKK-β that was extracted from TNF-α-stimulated HUVECs. We also found that p47 translocation, NOX4 expression, and reactive oxygen species (ROS) levels were up-regulated before IκB phosphorylation in TNF-α-induced HUVECs. Moreover, TNF-α-enhanced IKK-β activity was also inhibited by (polyethylene glycol) PEG-catalase, N-acetylcysteine (NAC), and vitamin E. In conclusion, these results suggest that AR reduces VCAM-1 and ICAM-1 expression through NADPH oxidase-dependent IKK/NF-κB pathways in TNF-α-induced HUVECs, which finally suppress monocyte-HUVECs adhesion. This compound is potentially beneficial for early-stage atherosclerosis. PMID:25463279

  16. The apoptotic effect of D Rhamnose β-hederin, a novel oleanane-type triterpenoid saponin on breast cancer cells.

    Directory of Open Access Journals (Sweden)

    Lin Cheng

    Full Text Available There is growing interest in development of natural products as anti-cancer and chemopreventive agents. Many triterpenoids have been proved as potential agents for chemoprevention and therapy of breast cancer. Ginsenosides from ginseng, which mostly belong to dammarane-type triterpenoids, have gained great attention for their anti-breast cancer activity with diverse mechanisms. However, studies of other kinds of triterpenoid saponins on breast cancer are limited. Previously, we purified and identified a novel oleanane-type triterpene saponin named D Rhamnose β-hederin (DRβ-H from Clematis ganpiniana, a Chinese traditional anti-tumor herb. In the present study, DRβ-H showed strong inhibitory activity on the growth of various breast cancer cells and induced apoptosis in these cells. DRβ-H inhibited PI3K/AKT and activated ERK signaling pathway. PI3K inhibitor LY294002 synergistically enhanced DRβ-H-induced apoptosis whereas MEK inhibitor U0126 reduced the apoptosis rate. Moreover, DRβ-H regulated the ratio of pro-apoptotic and anti-apoptotic Bcl-2 family proteins. Furthermore, DRβ-H induced depolarization of mitochondrial membrane potential which released Apaf-1 and Cytochrome C from the inter membrane space into the cytosol, where they promoted caspase-9 and caspase-3 activation. This is the first report on the pro-apoptotic effects of DRβ-H, a novel oleanane-type triterpenoid saponin, on breast cancer cells and its comprehensive apoptosis pathways. It implied that oleanane-type triterpenoid saponin DRβ-H could be a promising candidate for chemotherapy of breast cancer.

  17. Clematichinenoside (AR Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway

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    Haiyan Ding

    2016-05-01

    Full Text Available Mitochondria-mediated cardiomyocyte apoptosis is involved in myocardial ischemia/reperfusion (MI/R injury. Clematichinenoside (AR is a triterpenoid saponin isolated from the roots of Clematis chinensis with antioxidant and anti-inflammatory cardioprotection effects against MI/R injury, yet the anti-apoptotic effect and underlying mechanisms of AR in MI/R injury remain unclear. We hypothesize that AR may improve mitochondrial function to inhibit MI/R-induced cardiomyocyte apoptosis. In this study, we replicated an in vitro H9c2 cardiomyocyte MI/R model by hypoxia/reoxygenation (H/R treatment. The viability of H9c2 cardiomyocytes was determined by MTT assay; apoptosis was evaluated by flow cytometry and TUNEL experiments; mitochondrial permeability transition pore (mPTP opening was analyzed by a calcein-cobalt quenching method; and mitochondrial membrane potential (ΔΨm was detected by JC-1. Moreover, we used western blots to determine the mitochondrial cytochrome c translocation to cytosolic and the expression of caspase-3, Bcl-2, and Bax proteins. These results showed that the application of AR decreased the ratio of apoptosis and the extent of mPTP opening, but increased ΔΨm. AR also inhibited H/R-induced release of mitochondrial cytochrome c and decreased the expression of the caspase-3, Bax proteins. Conversely, it remarkably increased the expression of Bcl-2 protein. Taken together, these results revealed that AR protects H9c2 cardiomyocytes against H/R-induced apoptosis through mitochondrial-mediated apoptotic signaling pathway.

  18. Results of a screening programme to identify plants or plant extracts that inhibit ruminal protein degradation.

    Science.gov (United States)

    Selje, N; Hoffmann, E M; Muetzel, S; Ningrat, R; Wallace, R J; Becker, K

    2007-07-01

    One aim of the EC Framework V project, 'Rumen-up' (QLK5-CT-2001-00 992), was to find plants or plant extracts that would inhibit the nutritionally wasteful degradation of protein in the rumen. A total of 500 samples were screened in vitro using 14C-labelled casein in a 30-min incubation with ruminal digesta. Eight were selected for further investigation using a batch fermentation system and soya protein and bovine serum albumin as proteolysis substrates; proteolysis was monitored over 12 h by the disappearance of soluble protein and the production of branched SCFA and NH3. Freeze-dried, ground foliage of Peltiphyllum peltatum, Helianthemum canum, Arbutus unedo, Arctostaphylos uva-ursi and Knautia arvensis inhibited proteolysis (P Clematis vitalba and Erica arborea had little effect. Inhibition by the first four samples appeared to be caused by the formation of insoluble tannin-protein complexes. The samples were rich in phenolics and inhibition was reversed by polyethyleneglycol. In contrast, K. arvensis contained low concentrations of phenolics and no tannins, had no effect in the 30-min assay, yet inhibited the degradation rate of soluble protein (by 14 %, P < 0.0001) and the production of branched SCFA (by 17 %, P < 0.05) without precipitating protein in the 12-h batch fermentation. The effects showed some resemblance to those obtained in parallel incubations containing 3 mum-monensin, suggesting that K. arvensis may be a plant-derived feed additive that can suppress growth and activity of key proteolytic ruminal micro-organisms in a manner similar to that already well known for monensin. PMID:17445338

  19. Aurantiamide acetate suppresses the growth of malignant gliomas in vitro and in vivo by inhibiting autophagic flux.

    Science.gov (United States)

    Yang, Yi; Zhang, Li-hui; Yang, Bing-xian; Tian, Jin-kui; Zhang, Lin

    2015-05-01

    We aim to investigate the effect of aurantiamide acetate isolated from the aerial parts of Clematis terniflora DC against gliomas. Human malignant glioma U87 and U251 cells were incubated with different concentrations (0-100 μM) of aurantiamide acetate. Aurantiamide acetate greatly decreased the cell viability in a dose- and time-dependent manner. It induced moderate mitochondrial fragmentation and the loss of mitochondrial membrane potential. No significant difference was found in the alternation of other intracellular organelles, although F-actin structure was slightly disturbed. Apparent ultrastructure alternation with increased autophagosome and autolysosome accumulation was observed in aurantiamide acetate-treated cells. The expression of LC3-II was greatly up-regulated in cells exposed to aurantiamide acetate (P < 0.05 compared with control). The cytoplasmic accumulation of autophagosomes and autolysosomes induced by aurantiamide acetate treatment was confirmed by fluorescent reporter protein labelling. Administration of chloroquine (CQ), which inhibits the fusion step of autophagosomes, further increased the accumulation of autophagosomes in the cytoplasm of U87 cells. Autophagy inhibition by 3-methyladenine, Bafilomycin A1 or CQ had no influence on aurantiamide acetate-induced cytotoxicity, whereas autophagy stimulator rapamycin significantly suppressed aurantiamide acetate-induced cell death. The anti-tumour effects of aurantiamide acetate were further evaluated in tumour-bearing nude mice. Intratumoural injection of aurantiamide acetate obviously suppressed tumour growth, and increased number of autophagic vacuoles was observed in tumour tissues of animals receiving aurantiamide acetate. Our findings suggest that aurantiamide acetate may suppress the growth of malignant gliomas by blocking autophagic flux. PMID:25704599

  20. Clematichinenoside (AR) Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway.

    Science.gov (United States)

    Ding, Haiyan; Han, Rong; Chen, Xueshan; Fang, Weirong; Liu, Meng; Wang, Xuemei; Wei, Qin; Kodithuwakku, Nandani Darshika; Li, Yunman

    2016-01-01

    Mitochondria-mediated cardiomyocyte apoptosis is involved in myocardial ischemia/reperfusion (MI/R) injury. Clematichinenoside (AR) is a triterpenoid saponin isolated from the roots of Clematis chinensis with antioxidant and anti-inflammatory cardioprotection effects against MI/R injury, yet the anti-apoptotic effect and underlying mechanisms of AR in MI/R injury remain unclear. We hypothesize that AR may improve mitochondrial function to inhibit MI/R-induced cardiomyocyte apoptosis. In this study, we replicated an in vitro H9c2 cardiomyocyte MI/R model by hypoxia/reoxygenation (H/R) treatment. The viability of H9c2 cardiomyocytes was determined by MTT assay; apoptosis was evaluated by flow cytometry and TUNEL experiments; mitochondrial permeability transition pore (mPTP) opening was analyzed by a calcein-cobalt quenching method; and mitochondrial membrane potential (ΔΨm) was detected by JC-1. Moreover, we used western blots to determine the mitochondrial cytochrome c translocation to cytosolic and the expression of caspase-3, Bcl-2, and Bax proteins. These results showed that the application of AR decreased the ratio of apoptosis and the extent of mPTP opening, but increased ΔΨm. AR also inhibited H/R-induced release of mitochondrial cytochrome c and decreased the expression of the caspase-3, Bax proteins. Conversely, it remarkably increased the expression of Bcl-2 protein. Taken together, these results revealed that AR protects H9c2 cardiomyocytes against H/R-induced apoptosis through mitochondrial-mediated apoptotic signaling pathway. PMID:27248986

  1. Clematichinenoside attenuates myocardial infarction in ischemia/reperfusion injury both in vivo and in vitro.

    Science.gov (United States)

    Zhang, Rui; Fang, Weirong; Han, Dan; Sha, Lan; Wei, Jie; Liu, Lifang; Li, Yunman

    2013-09-01

    Clematichinenoside is a triterpenoid saponin isolated from the roots of Clematis chinensis. Oxidative stress and excessive nitric oxide production are thought to play considerable roles in ischemia/reperfusion injury that impairs cardiac function. The present study investigated the protective effect of clematichinenoside on regional and global ischemia/reperfusion injury and ventricular myocytes. In vivo, regional myocardial ischemia/reperfusion injury of rats was induced by the occlusion of the left anterior descending coronary artery, and isolated guinea pigs heart using Langendorff apparatus served as a global ischemia/reperfusion injury model ex vivo. Primary cultured neonatal ventricular myocytes were further applied to explore the anti-ischemia/reperfusion injury property in vitro. Infarct size was measured with TTC stain; enzyme activities such as lactate dehydrogenase, creatine kinase, superoxide dismutase, malondialdehyde, and nitric oxide were analyzed with assay kits; inducible nitric oxide synthase and endothelial nitric oxide synthase expressions were determined by Western blot. Clematichinenoside attenuated infarct size, decreased lactate dehydrogenase, creatine kinase, and malondialdehyde levels and enhanced superoxide dismutase activity. Clematichinenoside improved hemodynamics indexes, such as left ventricular developed pressure, maximum left ventricular developed pressure, and increase/decrease rate (± dp/dtmax) in the isolated guinea pig heart after reperfusion. Clematichinenoside also inhibited excessive production of nitric oxide through downregulating inducible nitric oxide synthase as well as upregulating endothelial nitric oxide synthase during ischemia/reperfusion injury. Clematichinenoside attenuates ischemia/reperfusion injury in vivo, ex vivo, and in vitro via an antioxidant effect and by restoring the balance between inducible nitric oxide synthase and endothelial nitric oxide synthase. PMID:23929248

  2. Ethnopharmacological survey of wild medicinal plants in Showbak, Jordan.

    Science.gov (United States)

    Al-Qura'n, S

    2009-05-01

    Two main research questions are framing this investigation: (1) the main taxa of the medicinal importance value altered the Showbak forest stand and species composition? (2) The most safe species and what are the toxic ones (unsafe). These two research questions are the vital ones to draw a clear image about the wild medicinal plants of this investigated area of Showbak region in Jordan. 79 wild medicinal plant species were investigated in this study which are used in traditional medication for the treatment of various diseases. Most of the locals interviewed dealt with well-known safe medicinal plants such as Aaronsohnia factorovskyi Warb. et Eig., Achillea santolina L., Adiantum capillus-veneris L., Artemisia herba-alba L., Ceratonia siliqua L., Clematis recta L., Herniaria hirsuta L., Malva neglecta Wallr., Rosmarinus officinalis L., Ruta chalepensis L., Salvia triloba L., Sarcopoterium spinosa (L.) Spach., Thymbra capitata (L.) Hof, and Urginea maritima Barker. Many of the wild medicinal plants investigated were toxic and needed to be practiced by practitioners and herbalists rather than the local healers. These plants include Calotropis procera Willd R.Br., Citrullus colocynthis (L.) Sch., Datura stramonium L., Digitalis purpurea L., Ecballium elaterium (L.) A.Rich., Euphorbia helioscopia L., Euphorbia tinctoria Boiss., Glaucium corniculatum (L.) Curt., Hyoscyamus aureus L., Mandragora officinarum L., Nerium oleander L., Ricinus communis L., Solanum nigrum L., Withania somnifera (L.) Dunel. The conservation of medicinal plants and natural resources is becoming increasingly important, so this research is trying to collect information from local population concerning the use of medicinal plants in Showbak; identify the most important specie; determine the relative importance value of the species and calculate the informant consensus factor (ICF) for the medicinal plants. Obtaining results is relied on the interviewee's personal information and the medicinal use

  3. In vitro antimalarial activity of extracts of some plants from a biological reserve in Costa Rica.

    Science.gov (United States)

    Chinchilla, Misael; Valerio, Idalia; Sánchez, Ronald; Mora, Víctor; Bagnarello, Vanessa; Martínez, Laura; Gonzalez, Antonieta; Vanegas, Juan Carlos; Apestegui, Alvaro

    2012-06-01

    Treatment with the usual antimalarial drugs, have induced parasite resistance, reinforcing the need to finding natural antimalarial components that would be found on plants from the forest. Therefore, we decided to look for these components in Costa Rican plants from a protected forest area. Fresh and dry extracts of roots, bark, leaves, flowers and fruits of 25 plants from a biological reserve in Costa Rica, Reserva Biol6gica Alberto Manuel Brenes (REBAMB), were studied in vitro for the presence of substances with antimalarial activity. By studying the inhibition of P berghei schizogony, we assessed the antimalarial activity of several plant extracts: Aphelandra aurantiaca, A. tridentata (Acanthaceae); Xanthosoma undipes (Araceae); Iriartea deltoidea (Arecaceae); Neurolaena lobata (Asteraceae); Senna papillosa, Pterocarpus hayessi, Lonchocarpus pentaphyllus (Fabaceae); Nectandra membranacea, Persea povedae, Cinamomum chavarrianum (Lauraceae); Hampea appendiculata (Malvaceae); Ruagea glabra, Guarea glabra (Meliaceae); Psidium guajava (Myrtaceae); Bocconia frutescens (Papaveraceae); Piper friedrichsthalii (Piperaceae); Clematis dioica (Ranunculaceae); Prunus annularis (Rosaceae); Siparuna thecaphora (Siparunaceae); Solanum arboreum, Witheringia solanacea (Solanaceae); Ticodendrum incognitum (Ticodendraceae); Heliocarpus appendiculatus (Tiliaceae) and Myriocarpa longipes (Urticaceae). We used different parts of the plants as well as fresh and dried extracts for testing IC50. The solid content of the extracts ranged from 1-71.9 microg/mL. The fresh extracts showed stronger activity than the dry ones. Since the plants showing the strongest antimalarial activity are very common in Central America, and some similar genera of these plants have shown positives results in South America, we considered important to present these findings for discussion. On the other hand, this is the first systematic study of this kind ever realized in a circumscribed and protected area of

  4. Diversity, Distribution and Prioritization of Fodder Species for Conservation in Kullu District, Northwestern Himalaya, India

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In the Indian Himalayan Region predominantly rural in character, livestock is one of the main sources of livelihood and integral part of the economy. Livestock mostly rely on fodder from wild.The diversity, distribution, utilization pattern, nativity,endemism, rarity, seasonality of availability, nutritive values, perceived economic values and pressure use index of livestock have not been studied. The present study attempts to enumerate 150 species of fodder representing trees (51 spp.), shrubs (54 spp.) and herbs (45 spp.). Poaceae (19 spp.) and Fabaceae (13 spp.) amongst families and Salix (6 spp.), Ficus,Clematis, and Desmodium (5 spp., each) amongst genera are rich in species. Maximum species were found in the 1801 ~ 2600 m zone, and the remaining two zones showed relatively low diversity. Out of the 150 species, 109 are used in summer, 5 winter and 36 throughout year. During rainy season, mostly grasses are used as fodder. Only 83 species are native to the Himalayan region, one species, Strobilanthus atropuroureus is endemic and 35 species are near endemic. The nutritive values of the fodder species were reviewed, and economic values and status of the species were also assessed. The pressure use index of the species was calculated on the basis of cumulative values of the utilization pattern,altitudinal distribution, availability, status, nativity and endemism. Amongst the species, Grewia oppositifoilia, Morus serrata, Indigofera heterantha,Quercus leucotrichphora, Ulmus villosa, U.wallichiana and Aesculus indica showed highest PUI indicating high preference and pressure. Season wise prioritization of the species for different altitudinal ones has been done. Appropriate strategy and action plan have been suggested for the conservation and management of fodder species.

  5. A comparison among root soil-conservation effects for nine herbs at the cold region highway in north-eastern China

    Science.gov (United States)

    Xu, W.; Wang, X.; Zhang, Y.; Liu, Y.

    2014-12-01

    High soil-conservation herbs are very important for slope vegetation restoration of a highway in serious sandstorm regions. In this study, nine common herbs in northeast China were selected and compared to study soil-conservation effects by using an undisturbed-soil trough scouring method for soil anti-scourability enhancement and hydrostatic collapse method for soil anti-erodibility. Further, principal components analysis was used to identify significant root features that affected soil erosion resistance. Results indicated that different herbs had distinct enhancement effects on soil erosion resistance. Soil anti-scourability enhancement index decreased with increases of soil depth, slope gradient and rainfall amount. Relationship between soil anti-erodibility enhancement index ( S) and immersion time ( t) is a cubic spline in each different herb type ( R 2 ≥ 0.88). Herb root features such as micro-aggregates, organic matter, net leaf weight, thick root length, fine root length and biomass contributed a leading role in soil erosion resistance enhancement effect, and all their common factor variances were more than 0.81. Descending order of soil erosion resistance enhancement effect in soil anti-scourability for nine herbs is Poa pratensis, Medicago sativa, Viola philippica, Rudbeckia hirta, Clematis heracleifolia, Kalimeris indica, Cosmos bipinnata, Hemerocallis fulva and Sedum elatinoides, while the sequence of soil anti-erodibility is M. sativa, S. elatinoides, P. pratensis, R. hirta, H. fulva, V. philippica, C. heracleifolia, C. bipinnata and K. indica. Therefore, we concluded that P. pratensis and M. sativa were the most suitable herbs for resisting soil erosion and recommended to be widely planted for road vegetation recovery in this region.

  6. 25 Years of Natural Product R&D with New South Wales Agriculture

    Directory of Open Access Journals (Sweden)

    I. Southwell

    2005-10-01

    Full Text Available Following recent NSW Government restructuring, the Department of Agriculture now exists in a composite form along with Forestry, Fisheries and Minerals in the new NSW Department of Primary Industries. This paper outlines some of the highlights of secondary metabolite R&D accomplished in the 25 years since the essential oil research unit was transferred from the Museum of Applied Arts & Sciences, Sydney to NSW Agriculture’s Wollongbar Agricultural Institute on the NSW north coast. The essential oil survey was continued, typing the Australian flora as a suitable source of isolates such as myrtenal (Astartea, myrtenol (Agonis, methyl chavicol (Ochrosperma, α-phellandren-8-ol (Prostanthera, methyl myrtenate (Darwinia, methyl geranate (Darwinia, kessane (Acacia, cis-dihydroagarofuran (Prosthanthera, protoanemonin (Clematis, isoamyl isovalerate (Micromyrtus, methyl cinnamate (Eucalyptus and bornyl acetate (Boronia. Many of these components are used, or have potential use in the fragrance, flavour, medicinal plant or insect attraction fields. Two weeds toxic to livestock in the Central West of the State are also harvested commercially as medicinal plants. Measurement of hypericin concentrations in the various plant parts of St John’s Wort (Hypericum perforatum over two seasons has shown that the weed can be effectively managed by grazing sheep during the winter months when toxin levels are low. Syntheses of β-carbolines tribulusterine and perlolyrine have shown that the former alkaloid was misidentified in the literature and hence not the toxic principle responsible for Tribulus staggers in sheep. Poor quality (high 1,8-cineole – low terpinen-4-ol oil bearing tea tree (Melaleuca alternifolia plantations have been established to the detriment of many a tea tree farmer. Analytical methods developed to check leaf quality at an early age indicated precursor sabinene constituents that convert to the

  7. [Essentials of pharmacophylogeny: knowledge pedigree, epistemology and paradigm shift].

    Science.gov (United States)

    Hao, Da-cheng; Xiao, Pei-gen; Liu, Li-wei; Peng, Yong; He, Chun-nian

    2015-09-01

    Chinese materia medica resource (CMM resource) is the foundation of the development of traditional Chinese medicine. In the study of sustainable utilization of CMM resource, adopting innovative theory and method to find new CMM resource is one of hotspots and always highlighted. Pharmacophylogeny interrogates the phylogenetic relationship of medicinal organisms (especially medicinal plants), as well as the intrinsic correlation of morphological taxonomy, molecular phylogeny, chemical constituents, and therapeutic efficacy (ethnopharmacology and pharmacological activity). This new discipline may have the power to change the way we utilize medicinal plant resources and develop plant-based drugs. Phylogenomics is the crossing of evolutionary biology and genomics, in which genome data are utilized for evolutionary reconstructions. Phylogenomics can be integrated into the flow chart of drug discovery and development, and extends the field of pharmacophylogeny at the omic level, thus the concept of pharmacophylogenomics could be redefined in the context of plant pharmaceutical resources. This contribution gives a brief discourse of knowledge pedigree of pharmacophylogeny, epistemology and paradigm shift, highlighting the theoretical and practical values of pharmacophylogenomics. Many medicinally important tribes and genera, such as Clematis, Pulsatilla, Anemone, Cimicifugeae, Nigella, Delphinieae, Adonideae, Aquilegia, Thalictrum, and Coptis, belong to Ranunculaceae family. Compared to other plant families, Ranunculaceae has the most species that are recorded in China Pharmacopoeia (CP) 2010. However, many Ranunculaceae species, e. g., those that are closely related to CP species, as well as those endemic to China, have not been investigated in depth, and their phylogenetic relationship and potential in medicinal use remain elusive. As such, it is proposed to select Ranunculaceae to exemplify the utility of pharmacophylogenomics and to elaborate the new concept

  8. НЕКОТОРЫЕ ОСОБЕННОСТИ РЕПРОДУКТИВНОЙ БИОЛОГИИ ВИДОВ РОДА С LEMATIS L. В УСЛОВИЯХ КУЛЬТУРЫ В БАШКИРСКОМ ПРЕДУРАЛЬЕ

    OpenAIRE

    ЖИГУНОВ ОЛЕГ ЮРЬЕВИЧ; НАСУРДИНОВА РОЗА АЛЬТАФОВНА

    2015-01-01

    В работе представлены результаты изучения особенностей биологии семян и семенная продуктивность некоторых видов рода Clematis в условиях культуры в Ботаническом саду-институте УНЦ РАН. Род Клематис - один из наиболее распространенных родов семейства Лютиковые ( Ranunculaceae Yuss.). В климатических условиях Ботанического сада-института г. Уфы из 20 таксонов, составляющих видовой коллекционный фонд, только у 10 видов успевают завязаться и вызреть семена: A. alpina, A. speciosa, C. hexapetala, ...

  9. СЕЗОННЫЙ РИТМ РАЗВИТИЯ НЕКОТОРЫХ СОРТОВ РОДА CLEMATISL. В УФИМСКОМ БОТАНИЧЕСКОМ САДУ

    OpenAIRE

    Жигунов, О.; Насурдинова, Р.; Никитина, Л.

    2011-01-01

    В работе представлены результаты изучения сезонного ритма развития 36 сортов рода Clematis L. в Ботаническом саду-институте УНЦ РАН. Сортовые крупноцветковые клематисы в условиях г. Уфы стабильно проходят все стадии сезонного развития, включая хорошую зимостойкость. По срокам цветения клематисы разделены на 3 группы: раннецветущие, среднецветущие, позднецветущие....

  10. СТРОЕНИЕ ПЛОДИКОВ НЕКОТОРЫХ ВИДОВ CIEMATISL., ИНТРОДУЦИРОВАННЫХ В ВОЛГОГРАДЕ

    OpenAIRE

    Нирода, А.; Сорокопудова, О.; Коротков, О.; Жолобова, О.

    2013-01-01

    Приведены результаты изучения морфологических особенностей плодиков 17 видов рода Clematis L. различного генетического происхождения. Изучены длина и ширина плодиков, их масса, длина стилодиев, форма, опушенность. Установлены отличия по величине плодиков, связанные с географическим местом интродукции ломоносов или их генетическим происхождением. Разработана 5-тикодовая шкала для оценки величины плодиков видов ломоносов с выделением очень мелких, мелких, средних, крупных и очень крупных. Выявл...

  11. The radioecology study of the biosphere territory of Issik-Kul

    International Nuclear Information System (INIS)

    covered ground (0,20 sm) the contents of uranium about from 1,1 up to 2,6x10-6 g/g, and with depth it is increased a few - to 3,0x10-6 g/g. there is more concentration of uranium in the average zone of Uranium deposits, where has the contents of uranium in the upper horizon of a soil equally 4,2x10-6 g/g, and in the bottom of horizon, on depth of 40-60 sm consist - 35,0x10-6 g/g or in 8,3 times above. According to some authors quantity of uranium in waters of the rivers Tone, Ae-Suu in hollow of Issyk-Kul makes 5,6x10-6 g/l, and in river Jergalan about (depend on seasonally and places of selection) from 2,8x10-6 to 1x10-5 g/l i.e. the main waters of wells and the rivers a hollow of Issyk-Kul contain in 10, and in some cases it is in 100 times more uranium, than water from black earth and not from black earth zones. On the surface of uranium tail deposits founded 10 sorts of plants of desert type: Ulmus pinnato-ramosa, Elaeagnus songorica, Caragana Turfanica, Peganum harmala, Akantholimon alatavicum, Natraria sibirica, Clematis songorica... The contents of uranium in the listed plants was small and changed about 0.02-0.31x10-6 g/g at coefficient of enrich is - from 1,0 to 15,5 and coefficient of discrimination is - from 17,8 to 240,0. Growing of plants in the environment with the high concentration of uranium not only is accompanied by change of theirs bioproductivity but also causes of morphological variability. The infringement of pigmentation of a flower at various sorts of plants is most often shown in area of Issyk Kul. The Astragalus borodinii is has morphological variability of splitting of a sheet plate. The Peganum garmala and Potentilla argentea - instead of usual 5 petals have 6-7 and their bifurcation. But other plants are intensive growing and developing (Caragana, Scutellaria, Peganum, and Peganum harmala). By the results of the research work the common level of external radiation in researched territory and level of uranium are about limits of the norm

  12. 威灵仙提取物干预膝骨关节炎软骨细胞的生长活力★%Effect of radix clematidis extract on viability of knee osteoarthritis chondrocytes

    Institute of Scientific and Technical Information of China (English)

    徐扬; 桂鉴超; 高峰; 徐燕; 王黎明; 陆一鸣; 尹昭伟

    2013-01-01

      背景:近年来研究显示关节软骨细胞过度凋亡是骨性关节炎开始和进展的关键因素,利用药物抑制软骨细胞凋亡来控制骨性关节炎的进展是现在的研究热点。而威灵仙在国内临床治疗骨关节炎时取得了较确切的疗效,但是其具体的作用机制是否通过抑制软骨细胞凋亡来实现尚未见相关报道。目的:观察中药威灵仙提取物对膝骨关节炎软骨细胞生长活力的影响。方法:取骨关节炎晚期行膝关节置换的关节软骨,剪碎后,采用酶消化法消化细胞,将处于对数生长期第3代细胞随机分组,实验组分别加入0.01,0.05,0.1,0.5,1.0 g/L威灵仙提取物培养基,对照组仅加入普通培养基进行培养。Live/Dead染色法测定人软骨细胞活力指数,TUNEL染色法测定人软骨细胞凋亡指数。结果与结论:0.05,0.1 g/L威灵仙提取物能明显提高软骨细胞活力,而0.5,1.0 g/L威灵仙提取物反而降低了软骨细胞活力,其活力指数与对照组比较差异均有显著性意义(P <0.05);0.01,0.05,0.1 g/L威灵仙提取物能有效抑制软骨细胞凋亡,1.0 g/L威灵仙提取物反而促进了软骨细胞凋亡,其凋亡指数与对照组比较差异均有显著性意义(P <0.05)。结果可见威灵仙提取物在合适的质量浓度时能有效提高人软骨细胞活力,抑制人软骨细胞凋亡,尤以0.1 g/L时效果最佳。%BACKGROUND:In recent years, studies have shown that excessive apoptosis of articular chondrocytes is the key factor for the start and progress of osteoarthritis. To study the progress in the use of drugs that inhibit apoptosis of chondrocytes to control osteoarthritis has become a hotspot. Clematis has obtained exact effect in the domestic clinical treatment of osteoarthritis, but the specific mechanism underlying inhibition of chondrocytes apoptosis has not been reported. OBJECTIVE:To observe the effect of radix