Sample records for clematis jackmanii

  1. A new triterpenoid saponin from Clematis ganpiniana

    Institute of Scientific and Technical Information of China (English)

    Feng Sun; Qing He; Pei Gen Xiao; Yi Yu Cheng


    A new triterpenoid saponin, named clematiganoside A (1), was isolated from the whole plant of Clematis ganpiniana. Its structure was elucidated on the basis of 1D, 2D NMR, TOF-MS and ESI-MS techniques, and physicochemical properties.

  2. Analysis of the Lightcurve of 1101 Clematis (United States)

    Warner, Brian D.; Stephens, Robert D.


    We report on our collaboration to obtain photometric data on the outer main-belt asteroid, 1101 Clematis. Data obtained in 2009 September yield a synodic rotation period of 34.3 ± 0.1 h and lightcurve amplitude of 0.16 ± 0.02 mag. The period spectrum shows a possible period at ~18.4 h but the phased lightcurve plot shows this solution is unlikely. The period of 34.3 h differs significantly from previously reported results.

  3. Identification of Xylem Occlusions Occurring in Cut Clematis (Clematis L., fam. Ranunculaceae Juss.) Stems during Their Vase Life (United States)

    Jedrzejuk, Agata; Rochala, Julia; Zakrzewski, Jacek; Rabiza-Świder, Julita


    During the vase life of cut stems obstruction of xylem vessels occurs due to microbial growth, formation of tyloses, deposition of materials in the lumen of xylem vessels and the presence of air emboli in the vascular system. Such obstructions may restrict water uptake and its transport towards upwards thus lowering their ornamental value and longevity of cut flowers. Clematis is a very attractive plant material which may be used as cut flower in floral compositions. Nothing is known about the histochemical or cytological nature of xylem blockages occurring in cut stems of this plant. This study shows that in clematis, tyloses are the main source of occlusions, although bacteria and some amorphic substances may also appear inside the vessels. A preservative composed of 200 mg dm−3 8-HQC (8-hydroxyquinolin citrate) and 2% sucrose arrested bacterial development and the growth of tyloses. This information can be helpful in the development of new treatments to improve keeping qualities of cut clematis stems. PMID:22919351

  4. Identification of Xylem Occlusions Occurring in Cut Clematis (Clematis L., fam. Ranunculaceae Juss. Stems during Their Vase Life

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    Agata Jedrzejuk


    Full Text Available During the vase life of cut stems obstruction of xylem vessels occurs due to microbial growth, formation of tyloses, deposition of materials in the lumen of xylem vessels and the presence of air emboli in the vascular system. Such obstructions may restrict water uptake and its transport towards upwards thus lowering their ornamental value and longevity of cut flowers. Clematis is a very attractive plant material which may be used as cut flower in floral compositions. Nothing is known about the histochemical or cytological nature of xylem blockages occurring in cut stems of this plant. This study shows that in clematis, tyloses are the main source of occlusions, although bacteria and some amorphic substances may also appear inside the vessels. A preservative composed of 200 mg dm−3 8-HQC (8-hydroxyquinolin citrate and 2% sucrose arrested bacterial development and the growth of tyloses. This information can be helpful in the development of new treatments to improve keeping qualities of cut clematis stems.

  5. A New Flavone C-Glycoside from Clematis rehderiana

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    Zhi-Zhi Du


    Full Text Available A new flavone C-glycoside, isovitexin 6″-O-E-p-coumarate (1 and two known flavonoid glycosides—quercetin 3-O-β-D-glucuronopyranoside (2 and isoorientin (3—were isolated from an ethanol extract of aerial parts of Clematis rehderiana. Their structures were determined by spectroscopic methods. The antioxidant effects of the two flavone C-glycosides were evaluated by both the MTT and DPPH assays. Compound 1 showed potent activities against H2O2-induced impairment in PC12 cells within the concentration range tested, whereas compound 3 scavenged DPPH radical strongly, with an IC50 value of 13.5 μM.


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    Valentin CODREANU


    Full Text Available Tipul morfologic anomocit al aparatelor (complexelor stomatice caracterizează epiderma abaxială şi adaxială a laminei frunzei la 5 specii de Clematis L. Densitatea stomatelor este specifică şi variază, în anul secetos 2007, de la 119,05 stomate/mm2 la specia Clematis vitalba până la 182,05 stomate/mm2 la specia C.tangutica.Grosimea medie mai mare a laminei frunzei şi prezenţa stomatelor în epiderma adaxială a laminei frunzei eviden­ţiază speciile mai rezistente la secetă. CANTITATIVE ANATOMY OF LEAF BLADE OF SOME CLEMATIS L. SPECIESAs a result of quantitative anatomic study of leaf blade of 5 species of genus Clematis L., is established that anomocytic morphological type of stomatal apparatus (complex is characteristic for the abaxial and adaxial epidermis. Stomatal density of leaf epidermis of clematises is specific, but varies in different years. The density of leaf stomata characterizes every species and varies, in drought 2007 year, from 119,05/mm2 at species Clematis vitalba to 182,05 stomata/mm2, at species C.tangutica. The greater average thickness of leaf blade and present of stomata in adaxial epidermis of leaf blade evidence more drought resistant species of clematises.

  7. Clematis austroanatolica (Ranunculaceae), an unusual new species from southern Anatolia, Turkey

    DEFF Research Database (Denmark)

    Zielinski, Jerzy; Tan, Kit


    Clematis austroanatolica (Ranunculaceae) is illustrated and described as a new species endemic to southern Anatolia, Turkey. It is related to C. vitalba and differs from the latter in having leaves with narrowly ovate, dentate-serrate leaflets, trifoliolate lowermost pinnae as well as small, pale...

  8. In vitro Antimicrobial Activity and Phytochemical Screening of Clematis Species Indigenous to Ethiopia. (United States)

    Hawaze, S; Deti, H; Suleman, S


    THE LEAVES EXTRACTS OF TWO INDIGENOUS PLANTS OF ETHIOPIA: Clematis longicauda steud ex A. Rich. and Clematis burgensis Engl. are used in Southwestern Ethiopia to treat otorrhoea and eczema. Antimicrobial activity and MIC of crude extracts were determined by disk diffusion and broth dilution. Phytochemical screening was performed on the extracts. The methanol and petroleum ether extracts of both plants showed antibacterial and antifungal activity. Sensitivity of reference strains was concentration dependent. Methanol and petroleum ether extracts of C. burgensis leaves exerted greater inhibitory effects than C. longicauda extracts whereas aqueous extracts of both plants were inactive. The MIC study revealed a concentration of 0.78 mg/ml on bacteria and 3.125 mg/ml on fungi for methanol extract and 1.56 mg/ml on both fungi and bacteria for petroleum ether extract. Phytochemical screening results indicated the presence of proteins, fixed oils, carbohydrates, tannins, saponins, flavonoids, and steroids. Preliminary chromatographic investigation showed fluorescing spots with R(f) values that ranged from 0.05 to 0.96 for phenolic compounds and saponins. As the study is one of the first reports on the two indigenous species of Clematis; isolation, purification and characterization of the different primary and secondary metabolites may further yield alternative options to the microbial chemotherapy.

  9. Triterpenoid Saponins from Clematis graveolens and Evaluation of their Insecticidal Activities. (United States)

    Rattan, Rajeev; Reddy, S G Eswara; Dolma, Shudh Kirti; Fozdar, Bharat Inder; Gautam, Veena; Sharma, Ritika; Sharma, Upendra


    A new hederagenin based triterpenoid saponin, clematograveolenoside A (1), along with three known saponins, tomentoside A (2), huzhangoside D (3) and clematoside S (4), were isolated from the roots and rhizomes of Clematis graveolens. The structure of new compound was elucidated on the basis of detailed analysis of chemical and spectroscopic data including 1D- and 2D NMR spectra. Compound 2 was found the most effective against aphid (Aphis craccivora) with an LC50 of 1.2 and 0.5 mg/mL after treatment for 72 and 96 h, respectively and was followed by compound 4 (LC50 = 2.3 and 1.9 mg/mL) and 1 (LC50 = 3.2 and 2.6 mg/mL). In case of termite (Coptotermis homii), compound 1 was found more toxic with an LC50 of 0.1 mg/L after 24 h of treatment followed by compound 2, 3 and 4 (LC50 = 0.1, 0.2 and 0.2 mg/mL, respectively).

  10. Chemical Constituents of Clematis chinensis Osbeck%威灵仙的化学成分研究

    Institute of Scientific and Technical Information of China (English)

    赵阳; 梁丽珍; 李厚金; 文诗雅; 蓝文健


    Clematis chinensis Osbeck is a popular medicinal plant. Seven known compounds, pinoresinol (1), epipinoresinol (2), matairesinol (3), salicifoliol (4), 3, 4, 5-trihydroxybenzoic acid (5), 4-hydroxy-3, 5-dimethoxybenzoic acid (6) , and 3-hydroxy- 4- methoxycinnamic acid (7) were isolated from the ethyl acetate extract of Clematis chinensis Osbeck by using the flash silica gel column chromatog-raphy and preparative high performance liquid chromatography. Their structures were elucidated mainly on the basis of NMR and MS data. These compounds could be fast and accurately detected from the extract by using HPLC and GCMS analytical methods. Lignans 1, 2, 3, 4 have various bioactivities, and the lignans from Clematis chinensis Osbeck should have the potential application in drug development.%威灵仙是一种重要的中药材.运用快速硅胶柱层析和制备型HPLC技术对威灵仙的乙酸乙酯提取物进行分离,纯化得到7个化合物,它们是松脂素( Pinoresinol,1),Epipinoresinol (2),罗汉松脂素(Matairesinol,3),Salicifoliol (4),3,4,5-三羟基苯甲酸(5),4-羟基-3,5-二甲氧基-苯甲酸(6),异阿魏酸(7),化合物的结构通过NMR和MS的分析得到确定.运用分析型HPLC和GC-MS色谱分析方法,可以在威灵仙药材提取物中快速、准确地鉴定该类化合物.化合物1,2,3,4是木脂素类化合物,具有多种重要的生理活性,潜在药用明显.

  11. Morphological and functional study of the marginal sphincter of the sea anemones Phymactis clematis and Aulactinia marplatensis from intertidal of Mar del Plata, Argentina Estudio morfológico y funcional del esfínter marginal de las anémonas de mar Phymactis clematis y Aulactinia marplatensis del intermareal de Mar del Plata, Argentina

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    Elvira A. González Oliveira


    Full Text Available It was made the characterization of marginal sphincter to the species Phymactis clematis (Drayton in Dana, 1849 and Aulactinia marplatensis (Zamponi, 1977, from intertidal ecosystem through their morphogical and functional study. The species P. clematis has a circumscript sphincter of palmate type. This muscle is constituted by a mesogloeal axis and several mesogloeal subaxes. Axis as well as subaxes give a support to the endoderm which border is smooth. Aulactinia marplatensis has a circunscript sphincter pinnate type. The axis has a truncated cone shape while in P. clematis the shape is cylindrical on its origin and it is bifurcated at the end. Both species experiments were carried out using the isolated muscles. They were stimulated at increasing KCl concentrations ranging from 20 to 200 mM. The results were analysed in the form of dose-response curves expressed in tension in grams force vs concentration. Contractil force increases in a sigmoid form to increasing KCl concentrations. The correlation between morphology and function and the differences shown in both species would be related to their intertidal distribution.Se realizó la caracterización de las anémonas de mar Phymactis clematis (Drayton in Dana, 1849 y Aulactinia marplatensis (Zamponi, 1977 del ecosistema intermareal mediante estudio morfológico y funcional. La especie P. clematis tiene un esfínter circunscripto de tipo palmado. Este músculo está constituido por un eje mesogloeal y varios subejes mesogloeales. Tanto el eje como los subejes dan soporte al endodermo cuyo borde es liso. La especie A. marplatensis tiene un esfínter circunscripto de tipo pinnado. El eje tiene forma de cono truncado mientras que en P. clematis es cilíndrico en su origen bifurcándose en su parte final. Los experimentos fueron llevados a cabo usando el músculo aislado de ambas especies. Estos fueron estimulados a concentraciones crecientes de KCl en un rango de 20 a 200 mM. Los resultados

  12. 小木通木脂素成分研究%Studies on Lignan Constituents of Clematis armandii Franch.

    Institute of Scientific and Technical Information of China (English)

    黄文武; 孔德云; 杨培明


    目的:研究小木通Clematis armandii Franch.的化学成分.方法:采用柱色谱进行分离纯化,通过理化方法和光谱分析鉴定化合物结构.结果:从小木通木质藤茎的乙醇提取物中分得9个木脂素成分,分别鉴定为armandiside(1),liriodendrin(2),(+)pinoresinol 4,4′-Ο-bis-β-D-glucopyranoside(3),(+)-pinoresinol 4′-Ο-β-D-glucopyranoside(4),(+)-syringa-resinol 4′-Ο-β-D-glucopyranoside(5),(+)-lariciresinol 4,4′-Ο-bis-β-D-glucopyranoside(6),(+)-lariciresinol 4-Ο-β-D-glucopyranoside(7),(+)-lariciresinol 4′-Ο-β-D-glucopyranoside(8),salvadoraside(9).结论:化合物1为新化合物,化合物2~9均为首次从该植物中分得.

  13. Identifying a contact zone between two phylogeographic lineages of Clematis sibirica (Ranunculeae) in the Tianshan and Altai Mountains

    Institute of Scientific and Technical Information of China (English)

    Hong-Xiang ZHANG; Ming-Li ZHANG


    Clematis sibirica,a woody vine occurring primarily under conifer forests,is widespread in northern Eurasia.In this study,we intend to illustrate how the taxon has responded in the area of the Tianshan and Altai Mountains of Central Asia to the Pleistocene climatic fluctuations.The chloroplast intergenic spacer psbA-trnH was sequenced for 125 individuals from 28 populations,and a total of eight chlorotypes were identified.The presence of definite phylogeographic structure was detected for the species (NST > GST,P < 0.001),and phylogenetic analysis indicated that the eight chlorotypes were clustered into two divergent lineages.They split at approximately 550-690 ka BP,according to coalescence analysis,coincident with the Pleistocene maximum glacial stage in these mountains,which suggests the restriction of these lineages to separate refugia at that time.Spatial analysis of molecular variance likewise divided the sampled populations into two associations,an Altai and eastern Tianshan group (populations 1-17),and a western Tianshan group (populations 18-28).Low levels of genetic diversity and unimodal mismatch distributions were obtained for both of these groups,suggesting postglacial range expansions.During the course of these expansions,mountain ranges surrounding the Dzungarian Basin probably served as migration corridors.In addition,a contact zone was identified in the central Tianshan and eastern Altai Mountains between the two phylogeographic lineages.

  14. Research on the anti-inflammation and analgesic effect of different processed products of Clematis%威灵仙不同炮制品抗炎镇痛作用的研究

    Institute of Scientific and Technical Information of China (English)

    刘仕琦; 李菲; 李俊莲; 罗秀夏


    Objective:To evaluate the anti-inflammation and analgesic effect of different processed products of Clematis. Methods:The analgesic and the anti-inflammation effects of different processed products of Clematis were studied by writhing response,exploring in the xylene-induced ear swelling model in mice,and egg white-induced paw edema in rats. Results:Clematis by stir-heating with rice vinegar can significantly prolonge the latency and reduce the writhe number at the turning of trunk model in Kun-Ming mice(P<0.01). Clematis,Clematis by stir-heating with rice vinegar and wine have good anti-inflammatory effect on inhibiting xylene-induced ear swelling (P<0.01). Clematis by stir-heating with rice vinegar can also reduce rats foot-plate swelling in 2 hour (P<0.05). Conclusion:The results demonstrate that different processed products of Clematis can release the pain,suppress the inflammation. The effect of Clematis by stir-heating with rice vinegar is the best.%目的:研究威灵仙生品及不同炮制品的抗炎镇痛作用。方法:选用醋酸扭体实验、二甲苯诱导小鼠耳肿胀实验和蛋清致大鼠足肿胀实验,观察威灵仙生品及不同炮制品的抗炎作用。结果:醋炙威灵仙能明显减少冰醋酸所致的小鼠扭体次数(P<0.01);生品威灵仙、醋炙威灵仙、酒炙威灵仙均能明显抑制二甲苯引起的小鼠耳廓肿胀(P<0.01);醋炙威灵仙在造模后2 h能明显抑制蛋清所致大鼠足肿胀(P<0.05)。结论:威灵仙生品及酒炙、醋炙品均具有一定的抗炎、镇痛作用,其中醋炙品作用效果最好。

  15. Clematis L.Resources Research and Applied Analysis%铁线莲属植物资源及其研究与应用价值分析

    Institute of Scientific and Technical Information of China (English)

    亚里坤·努尔; 买买提江·吐尔逊; 吐尔逊古丽·托乎提


    Discusses the Chinese Clematis L.resources,system classification,cultivation of reproduction,breeding,garden and other aspects of application of the status quo.Clematis is a kind of ornamental value,with a variety of resistance of the vine,of about 147.If through the introduction and domestication of these species or hybrid breeding,increased reproduction,and then applied to the urban landscape to,will greatly enrich our climbing plant species resources.Many species spend a large colorful,rich color,pattern changing,long flowering,ornamental value is high,with "Queen of Climbing Plants"reputation,Vertical garden in the garden can increase the diversity of green.From strengthening management,active protection,the establishment of the introduction and domestication of wild clematis base development and utilization of proposals put forward and elaborated in the future in the physiological ecology,introduction and domestication,breeding,garden and other aspects of applied research focus and direction.%论述了中国铁线莲属植物种质资源、系统分类、栽培繁殖、新品种选育、园林应用等方面的研究现状。从加强管理、积极保护、建立野生铁线莲的引种驯化基地等方面提出了开发利用建议,并阐述了今后在生理生态、引种驯化、新品种选育、园林应用研究等方面的研究重点和方向。

  16. Adaptability of Eight Small-Flower Cultivated Varieties of Clematis L.%铁线莲8个小花品种的适生性比较

    Institute of Scientific and Technical Information of China (English)

    孟雪; 郝笑微; 王锦


    [目的]为筛选出适合昆明地区栽培的铁线莲(Clematis L.)品种,对从欧洲引进的8个小花品种的适生性进行研究.[方法]对8个小花品种(‘Hakuree’、‘Bluish violet’、‘Freda’、‘Markham's Pink’、‘Edis6900’、‘Eighteen Anna’、‘Japonica,、'Victoria,)主要进行萌芽期、现蕾期和初花期的观测及观赏价值的评价、抗病虫害能力等级的评定和适生性等级综合评定等研究.[结果]' Freda,、'Victoria,和‘Eighteen Anna’为适生型,能够正常开花,植株高度较高,攀爬效果较好,是较好的攀援植物,适宜在昆明推广应用;'Edis6900,可以定为较适生型,植株能够正常开花,花的直径较大,花朵的观赏效果较好,但攀援能力较差;‘Hakuree,、'Bluish violet,、'Markham's Pink,和‘Japonica’为不适生型,植株不能正常开花,表现出在昆明的不适生状态.[结论]该研究可为铁线莲属植物在昆明地区的推广应用提供参考,为铁线莲商品化生产提供栽培、管理提供理论依据.%[Objective] In order to select suitable cultivated varieties of Clematis L. In Kunming Area, adaptability of eight small-flower cultivated varieties of Clematis L. Were studied. [ Method] The germination period, budding stage, early flowering stage of eight small-flower cultivated varieties ( ' Hakuree' ,' Bluish violet' ,' Freda' ,' Markham ' s Pink',' Edis6900,' Eighteen Anna',' Japonica' ,' Victoria' ) were ovserved, and their ornamental value, ability of resistance to disease and pest and adaptability assessment were carried out. [ Result ]' Freda' ,' Victoria' ,' Eighteen Anna' can bloom normally,are better climbing plants that have higher height and better climbing effect in Kunming,so they are suitable types and are good for popularization and application in Kunming. ' Edis6900' can bloom normally, meanwhile its flowers have larger diameter and better viewing effect, but its climbing ability is bad in Kunming, so it

  17. Comparison on Four Extraction Methods of Genomic DNA from Clematis fasciculiflora Franch%4种滑叶铁线莲基因组DNA提取方法比较

    Institute of Scientific and Technical Information of China (English)

    胡祎晨; 孙正海; 王锦; 李世峰; 辛培尧; 范萱


    [Objective ] This study aimed at comparing the four extraction methods of genomic UNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch. [ Method] Leavies of Clematis fas-cicidiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ , improved CTAB method Ⅱ , improved CTAB method Ⅲ and improved SDS method. [Result] The four extraction methods could all successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch. The purity of genomic DNA was the highest using improved CTAB method I , with the longest extracting time; while the concentration of genomic DNA was the maximum using the improved SDS method, with the shortest extracting time and relatively low purity; the extracting time of improved CTAB method Ⅲ was the shortest. [ Conclusion ] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.%[目的]对4种滑叶铁线莲基因组DNA提取方法进行比较研究,建立滑叶铁线莲最适DNA提取方法.[方法]以滑叶铁线莲叶片为材料,比较改良CTAB法Ⅰ、改良CTAB法Ⅱ、改良CTAB法Ⅲ、改良SDS法这4种基因组DNA提取法在提取DNA纯度、浓度和提取时间等方面的不同.[结果]4种方法都可提取滑叶铁线莲基因组DNA.改良CTAB法Ⅰ提取DNA纯度最高,但浓度最低且提取时间最长;改良SDS法提取DNA浓度最高,所需时间较短,但纯度较低;改良CTAB法Ⅲ提取所需时间最短.[结论]建立了铁线莲最适DNA提取方法,为运用分子生物学手段对其研究提供支持.

  18. Comparison on Four Extraction Methods of Genomic DNA from Clematis fasciculiflora Franch%4种滑叶铁线莲基因组DNA提取方法比较

    Institute of Scientific and Technical Information of China (English)

    胡祎晨; 孙正海; 王锦; 李世峰; 辛培尧; 范萱


    [目的]对4种滑叶铁线莲基因组DNA提取方法进行比较研究,建立滑叶铁线莲最适的DNA提取方法。[方法]以滑叶铁线莲叶片为材料,比较改良CTAB法Ⅰ、改良CTAB法Ⅱ、改良CTAB法Ⅲ、改良SDS法这4种基因组DNA提取法在提取的DNA纯度、浓度和提取时间等方面的不同。[结果]4种方法都可提取滑叶铁线莲基因组DNA。改良CTAB法Ⅰ提取DNA纯度最高,但浓度最低且提取时间最长;改良SDS法提取DNA浓度最高,所需时间较短,但纯度较低;改良CTAB法Ⅲ提取所需时间最短。[结论]建立了铁线莲最适DNA提取方法,为运用分子生物学手段对其研究提供支持。%[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch.[Method] Leavies of Clematis fasciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ,improved CTAB method Ⅱ,improved CTAB method Ⅲ and improved SDS method.[Result] The four extraction methods could all be successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch.The purity of genomic DNA was the highest using improved CTAB method Ⅰ,with the longest extracting time;while the concentration of genomic DNA was the maximum using the improved SDS method,with the shortest extracting time and relatively low purity;the extracting time of improved CTAB method Ⅲ was the shortest.[Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.

  19. Study on the Chemical constituents of a Traditional Mogolian Medicine HERBA CLEMATIS%蒙药材细叶铁线莲化学成分研究*

    Institute of Scientific and Technical Information of China (English)

    包保全; 乌雅罕; 陈建平; 布仁; 包娜; 张屏


    This study was aimed to isolate and characterize of chemical constituents in a traditional Mongolian medicine HERBA CLEMATIS. Normal and reverse phase coloumn chromatography, gel filtration chromatography sephadex LH-20, and preparative HPLC were used for isolation and purification compounds from the water extraction of the arial part ofC. aethusaefoliaTurcz. The planar structures and spatial configurations of isolated compounds were identified by high resolution MS, 1D and 2D NMR, and other spectrographic methods. The chemical research on the Mongolian medicine results 6 compounds, dihydrodehydrodiconiferyl alcohol (1), syringaresinol (2), pinoresinol (3), epi-pinoresinol (4), lirioresinol B dimethyl ether (5) and loliolide (6). All the compounds were isolated from this plant for the first time.%目的:研究蒙药材细叶铁线莲(HERBA CLEMATIS)中主要化学成分。方法:利用正、反相柱色谱,凝胶色谱LH20和制备型HPLC等方法,对芹叶铁线莲(Clematis aethusaefoliaTurcz.)干燥地上部分的水提取物进行分离纯化;得到的单体化合物经过高分辨质谱(HRMS)、核磁共振(1D、2D-NMR)等光谱方法,鉴定其平面结构和立体构型。结果:从细叶铁线莲水提取物中分离得到6个化合物,分别鉴定为二氢去氢二聚松柏醇(1),丁香脂素(2),松脂素(3),表松脂素(4),里立脂素B二甲醚(5)和黑麦草内酯(6)。结论:所有化合物均为首次从该植物中分离得到。

  20. Proteomic and Metabolomic Analyses of Leaf from Clematis terniflora DC. Exposed to High-Level Ultraviolet-B Irradiation with Dark Treatment. (United States)

    Yang, Bingxian; Wang, Xin; Gao, Cuixia; Chen, Meng; Guan, Qijie; Tian, Jingkui; Komatsu, Setsuko


    Clematis terniflora DC. has potential pharmaceutical value; on the contrary, high-level UV-B irradiation with dark treatment led to the accumulation of secondary metabolites. Metabolomic and proteomic analyses of leaf of C. terniflora were performed to investigate the systematic response mechanisms to high-level UV-B irradiation with dark treatment. Metabolites related to carbohydrates, fatty acids, and amino acids and/or proteins related to stress, cell wall, and amino acid metabolism were gradually increased in response to high-level UV-B irradiation with dark treatment. On the basis of cluster analysis and mapping of proteins related to amino acid metabolism, the abundances of S-adenosylmethionine synthetase and cysteine synthase as well as 1,1-diphenyl-2-picrylhydrazyl scavenging activity were gradually increased in response to high-level UV-B irradiation with dark treatment. Furthermore, the abundance of dihydrolipoyl dehydrogenase/glutamate dehydrogenase and the content of γ-aminobutyric acid were also increased following high-level UV-B irradiation with dark treatment. Taken together, these results suggest that high-level UV-B irradiation with dark treatment induces the activation of reactive oxygen species scavenging system and γ-aminobutyric acid shunt pathway in leaf of C. terniflora.

  1. 国产毛茛科银莲花族十七种植物的细胞学研究%Cytology of ten species in Anemone,one in Anemoclema and six in Clematis (Trib. Anemoneae, Ranunculaceae) from China

    Institute of Scientific and Technical Information of China (English)



    研究了国产毛茛科银莲花族Trib. Anemoneae 17种植物的染色体数目和核型.10种银莲花属Anemone L.植物中,1种(西南银莲花A. davidii)为x=8的四倍体(2n=4x=32),5种 (匍枝银莲花A. stolonifera、草玉梅A. rivularis、卵叶银莲花A. begoniifolia、水棉花 A. hupehensis f. alba、大火草A. tomentosa)为x=8的二倍体 (2n=2x=16), 4种 (鹅掌草A. flaccida、湿地银莲花A. rupestris、蓝匙叶银莲花A. trullifolia var. colestina、拟条叶银莲花A. trullifolia var. holophylla、展毛银莲花A. demissa) 为x=7 的二倍体 (2n=2x=14).罂粟莲花 Anemoclema glaucifolium为x=8 的二倍体.6种铁线莲属Clematis L. 植物 (滇川铁线莲C. kockiana、长花铁线莲 C. rehderiana、毛茛铁线莲 C. ranunculoides、扬子铁线莲C. puberula var. ganpiniana、短尾铁线莲 C. brevicaudata、金毛铁线莲 A. chrysocoma)均为 x=8的二倍体.银莲花属中x=7的种类的核型彼此十分相似,均由6对大型具中部着丝点的染色体和1对具端部着丝点的染色体组成;x=8的二倍体种类的核型与罂粟莲花属和铁线莲属植物的核型十分相似,均由5对大型具中部着丝点和3对具端部或近端部着丝点的染色体组成.%The somatic chromosome number and detailed chromosome morphology have been studied in ten species of Anemone, one species of Anemoclema, and six species of Clematis, all from China, namely Anemone davidii Franch. (2n=4x=32), A. stolonifera Maxim. (2n=2x=16), A. flaccida Fr. Schmidt (2n=2x=14), A. rivularis Buch.-Ham. (2n=2x=16), A. begoniifolia Lévl. et Vant. (2n=2x=16), A. hupehensis Lem. f. alba W. T. Wang (2n=2x =16), A. tomentosa (Maxim.) Péi (2n=2x=16), A. rupestris Hook. f. et Thoms. (2n=2x=14), A. trullifolia var. colestina (Franch.) Finet et Gagnep. (2n=2x=14), A. trullifolia var. holophylla Diels (2n=2x=14), A. demissa Hook. f. et Thoms. (2n=2x=14), Anemoclema glaucifolium (Franch.) W. T. Wang (2n=2x=16), Clematis kockiana Schneid. (2n=2x=16

  2. 光照和温度对毛茛铁线莲种子萌发的影响%Effect of Light and Temperature on Seed Germination of Clematis ranunculoides

    Institute of Scientific and Technical Information of China (English)

    牛红彬; 关文灵; 李世峰; 宋杰


    In order to study the effect of light and temperature on seed germination of Clematis ranunculoides and its basic biological characteristics, its wild plant seeds were collected, moreover, the seed’s appearance was observed, and the thousand-seed weight was detected. The laboratory test method was used to determine the germination rate and potentiality under different conditions of light and temperature. The results showed that the thousand-seed weight of C. ranunculoides was 1.6624± 0.0006 g. The optimal temperature for the seed germination was 20~25℃, and at this temperature, the seed begin to germinate in 8 days after seeding, and the germination procedure finished in 3~4 weeks, with the germination rate of 90.33%. Both higher and lower temperature could cause the decrease of germination rate, while light and dark did not influence significantly on the germination rate of C. ranunculoides seed.%  采集毛茛铁线莲(Clematis ranunculoides)野生植株种子,观察其外观形态,检测种子千粒重,研究光照和温度对种子萌发的影响.通过室内发芽试验,测定不同光照和温度条件下毛茛铁线莲种子发芽率和发芽势.结果表明,毛茛铁线莲种子的千粒重为(1.6624±0.0006) g;种子萌发最适温度为20~25℃,在此温度下,8 d开始萌发,3~4周萌发完全,萌发率达90.33%,温度升高或降低都会导致毛茛铁线莲种子萌发率降低;光照和黑暗对毛茛铁线莲种子萌发无显著影响.

  3. Studies on microsporogenesis and male gametophyte formation of Clematis hexapetala%棉团铁线莲小孢子发生和雄配子体的形成

    Institute of Scientific and Technical Information of China (English)

    全雪丽; 刘继生; 吴松权


    采用石蜡切片技术,观察棉团铁线莲小孢子发生和雄配子体的形成过程。结果表明:棉团铁线莲每个花药横切面呈蝶形,具4个花粉囊;小孢子在四分体中的排列以四面体型为主;成熟花粉粒近球形,属2-细胞型,具有3条萌发沟;花粉囊壁由4层细胞构成,即表皮(1层)、药室内壁(1层)、中层(数层)、绒毡层(1层);绒毡层发育属分泌型。%The procedures of microsporogenesis and male gametophyte formation of Clematis hexapetala were observed by traditional paraffin section technique.The results indicated that the cross-section of anther was butterfly-shaped,each anther included four pollen sacs;microspore were tetrahedron-shaped;mature pollens were subglobose,two-cell type,with three germinal furrows;wall of pollen sac was consisted by four layers,i.e.epidermis,endothecium,middle layer and tapetum;tapeta were secretor type.

  4. Study on Suitable Distribution Areas of Kawa Kimichi Produced in Sichuan Province Based on Remote Sensing and GIS—A Case Study of Clematis Armandii%基于遥感与GIS技术的四川道地药材川木通分布研究—以小木通为例

    Institute of Scientific and Technical Information of China (English)

    董丽君; 尚雪; 文路军; 彭文甫; 徐新良; 方清茂


    本文根据四川道地药材小木通的生长适宜性要求,通过遥感与GIS空间分析、空间数据建库等技术,提取土地利用信息,结合小木通生长的环境指标进行量化和综合分析,获得四川道地药材小木通的适宜分布区域.研究结果表明,四川省古蔺县、叙永县、宝兴县和天全县等是小木通的适宜分布区域,其中古蔺、叙永两县是主要分布区域,分别占适宜区总面积的23.2%和25.4%.通过与中药资源数据库对比可知,本次研究结果与第三次全国中药普查数据基本吻合.这表明利用 GIS与遥感技术对小木通适宜区进行研究具有科学性和可行性,可提高小木通的合理利用,为四川道地药材小木通的合理种植提供信息支持,为其它野生中药材道地区域监测提供参考.%The suitable distribution regions of medicinal clematis armandii in Sichuan province were extracted based on the suitable requirements of clematis armandii,comprehensive analysis and quantification on environmental indicators suitable forclematis armandii,following remote sensing (RS) and GIS spatial analysis,spatial database building and other technologies,extracted land use information.The results showed that regions suitable forclematis armandii were Gulin county,Xuyong county,Baoxing county and Tianquan county and other areas in Sichuan province.Among them,Gulin county and Xuyong county were the main distributed regions,which accounted for 23.2% and 25.4% of the total area,respectively.Through the comparison with the Chinese Materia Medica (CMM) Resource Database,this research result was basically coincided with census data from the Third National CMM.It also showed that RS and GIS technology were suitable for the investigation of suitable area of clematis armandii scientifically and feasibly.It improved the rational use of clematis armandii.It provided supporting information for rational planting of clematis armandii.It also of fered references

  5. 威灵仙注射液关节腔离子导入对骨关节炎软骨和滑膜组织形态以及软骨MMP-1的影响%Effects of clematis chinensis injection on OA cartilage and synovial membrane tissue morphology and MMP-1 cell with articular cavity iontophoresis

    Institute of Scientific and Technical Information of China (English)

    孙必强; 张鸣; 李美珍; 李果丽


    目的:研究威灵仙注射液关节腔离子导入对OA的软骨和滑膜组织形态和软骨MMP-1细胞凋亡的影响。方法:新西兰兔40只,除保留10只作为正常组外,余均采用木瓜蛋白酶关节腔注射复制OA模型。造模4周后,根据模型动物关节肿胀度随机分为模型组、Wlx组、Qand组。实验结束时,取模型动物关节软骨和滑膜进行病理形态学检查,同时观察软骨细胞凋亡情况。结果:威灵仙关节腔离子导入能明显降低模型动物关节软骨和滑膜的病理总积分,同时亦能明显降低软骨 MMP-1细胞的阳性表达率。结论:威灵仙关节腔离子导入可能具有抑制骨关节炎的软骨退行性改变的作用,能够延缓OA的炎症进程。%Objective:To study effects of Clematis chinensis injection on OA cartilage and synovial membrane tissue morphology and apoptosis of MMP-1 cell with articular cavity iontophoresis. Methods:40 New Zealand rabbits, except 10 rabbits as normal group, the others were used to replicate OA model by papain intra-articular injection. After 4 weeks modeling, according to the degree of joint swelling, model animals were randomly divided into model group, Wlx group, Qand group. At the end of the experiment, examinated pathological total points of articular cartilage and synovium membrane, the cartilage cell apoptosis was observed. Results:Clematis articular iontophoresis can significantly reduce articular cartilage and synovial pathological score of model animals, but also can significantly reduce the positive expression rate of MMP-1 cell of cartilage. Conclusion: Clematis articular iontophoresis may inhibit osteoarthritis cartilage degeneration, can slow down the inflammatory process of OA.

  6. Experimental study on external application of Clematis chinensis extract after acupuncture treatment in Japanese big ear rabbits with experimental knee osteoarthritis%电针后外敷威灵仙浸膏治疗日本大耳白兔实验性膝骨关节炎的研究

    Institute of Scientific and Technical Information of China (English)

    李敏; 丁武华; 李莉; 方伟; 郭俐宏


    目的:研究电针配穴联合自制威灵仙浸膏外敷治疗实验性日本大耳白兔膝骨关节炎合并关节腔积液的效果及作用机制。方法将日本大耳白兔36只随机分为模型组、电针组、威灵仙组及联合组,4组均于第1,3,7天各注射1次弗氏完全佐剂(0.1 mL)于左膝关节腔内造模;造模成功后电针组取足三里、血海、阳陵泉为主穴电针刺激30 min,威灵仙组于膝骨关节处每日涂抹威灵仙浸膏,联合组在电针足三里、血海、阳陵泉30 min后膝骨关节处每日涂抹威灵仙浸膏,均治疗2周。分别于造模前及治疗后2周测定关节积液中炎症递质前列腺素E2( PGE2)、透明质酸( HA)、白细胞介素-1β( IL-1β)水平;计算足趾关节肿胀率及消肿时间,并于治疗结束后取动物关节软骨测定软骨MMP-1细胞阳性表达率。结果治疗2周后,联合组PGE2、IL-1β水平,MMP-1细胞阳性表达率及足趾关节肿胀率均明显低于治疗前及电针组、威灵仙组(P均<0.05),消肿时间明显短于电针组、威灵仙组(P均<0.05),HA水平高于电针组、威灵仙组(P均<0.05)。结论电针配穴联合自制威灵仙浸膏外敷治疗日本大耳白兔实验性膝骨关节炎合并关节腔积液效果好。其作用机制可能是电针刺激可激发经气,降低痛阈,威灵仙浸膏经皮吸收后可促进局部血液循环及局部病变组织新陈代谢,增加HA分泌,抑制IL-1β的合成,二者协同促使病变组织恢复,减轻关节疼痛。%Objective It is observe the effect of electro acupuncture combined with self-made Clematis extract by external application on the Japanese big ear rabbits of experimental knee osteoarthritis (KOA)with joint effusion, and to explore its anti inflammation mechanism .Methods 36 white rabbits were randomly divided into model group , electro-acupuncture group , Clematis group and

  7. Mutis o la trampa de la Mutisia Clematis

    Directory of Open Access Journals (Sweden)

    Ángela Pérez Mejía


    Full Text Available Existen muchas pinturas y esculturas que recuerdan a José Celestino Mutis. No en vano pasó veintidós años rodeado de una tropa de pintores, dibujando la flora de la Nueva Granada. También él quedó eternizado en varias pinturas y ahora preside salones de museos y universidades, de tal manera que se puede suponer que la imagen es inmediatamente reconocible para los colombianos. Hay una pintura particularmente común que adornaba mi libro de ciencias naturales de tercero elemental. Era la pintura de un hombre viejo suspendido en un pedestal de mármol, rodeado de instrumentos de medición y libros de anotaciones botánicas. Una planta enredadera, que parece salida de uno de los libros que rodean al personaje, subía por la base del busto y delicadamente se posaba sobre su cuerpo. Debajo se leía: "Mutis: sabio y precursor de la independencia"

  8. Clematis Hill Landscape of the Sculpture Garden Museum of VANGI%梵吉雕塑园美术馆

    Institute of Scientific and Technical Information of China (English)


    @@ 本设计是为专门陈列意大利现代最著名具象派雕塑大师朱利亚诺·梵吉(Giuliano Vangi)作品的美术馆而做的园林设计.用地付于富士山麓广袤的丘陵地带,可俯瞰箱根的外轮山并远眺伊豆半岛的群山,自然风景明媚.这里原为一座拥有30多年历史的庄园中的体育俱乐部,为纪念梵吉而进行再开发,定位为可享受艺术、花木和美食的大众休闲场所.在这座名为"铁线莲"的山丘上,还建有巴菲特美术馆、井上靖文学馆和木村圭吾美术馆等展馆设施,而其核心就是"梵吉雕塑园美术馆".

  9. 东北铁线莲种胚后熟过程中内源激素动态变化%Morphological Development of Seed Embryo in Its Post-ripening and Dynamic Changes of Endogenesis Hormone in Clematis Mandshurica

    Institute of Scientific and Technical Information of China (English)

    许世泉; 王振兴; 邵财; 王志清; 逄世峰; 张瑞


    用植物激素的酶联免疫吸附测定法(ELISA)测定不同时期东北铁线莲种子内源激素IAA、ABA、GA、ZR的动态变化.结果表明:低温层积初期东北铁线莲种子ABA的含量呈较明显的下降趋势;进人生理后熟期以后,GA、ZR的含量持续升高,抑制萌发物质减少及促萌物质的增加与积累,最终导致东北铁线莲种胚休眠的解除.%To investigate dynamic changes of endogenesis hormone IAA, ABA, GA, ZR in its post-ripening by ELISA in the seed embryo post-ripening. At early stage of low temperature lamination, the ABA content of the seeds was on a declining curve and quite evident. The GA, ZR content persistently increased after physiology after-ripening. The reduction of inhibitory substances, and increment and accumulation of promoting germination substances led to dormancy-release of seed embryo.

  10. Study the Condition for Extraction of Total Flavoniods from Clematis Filamentosa Dunn by Orthogonal Test%正交试验法研究甘木通总黄酮的提取条件

    Institute of Scientific and Technical Information of China (English)

    胡宗礼; 黄晓萍; 陈珺霞



  11. Severe contact dermatitis and systemic reaction caused by fresh Clematis chinensis osbeck:A case report%鲜威灵仙致重症接触性皮炎和全身性不良反应 1例

    Institute of Scientific and Technical Information of China (English)

    王乃忠; 薛筑云; 毕志刚; 雷铁池; 何怡


    患者女 , 30岁 , 大面积外敷鲜威灵仙全草 10小时后 , 接触部发生红斑、大疱和灼痛伴心悸、胸闷等全身性不良反应 , 持续 6天 , 经治疗痊愈 . 斑贴试验证实鲜威灵仙全草系原发性刺激物 .

  12. 威灵仙总皂苷对佐剂性关节炎大鼠踝关节病理的影响%Effect of total saponins of Clematis on the pathological changes of ankles in adjuvant arthritis rats

    Institute of Scientific and Technical Information of China (English)

    徐先祥; 夏伦祝; 张睿


    目的:观察威灵仙总皂苷对佐剂性关节炎(AA)大鼠踝关节病理改变的影响.方法:大鼠足趾皮内注射弗氏完全佐剂致佐剂性关节炎,威灵仙总皂苷按25、50、100 mg/kg剂量灌胃给药15天,观察大鼠踝关节滑膜、关节腔、软骨、周围软组织等组织病理情况.结果:威灵仙总皂苷(50、100 mg/kg)明显减轻AA大鼠踝关节病理损伤.结论:威灵仙总皂苷可改善AA大鼠的踝关节组织病理改变.

  13. 响应面法优化黄花铁线莲总皂苷超声提取工艺%Optimization of ultrasound extraction technology of total saponins from Clematis intricata Bunge by response surface method

    Institute of Scientific and Technical Information of China (English)

    赵科; 解红霞; 张娜



  14. Drug: D06694 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available gs D06694 Clematis root (JP16) Traditional Chinese Medicine in Japan [BR:br08304] Crude Drugs Drugs for damp...ness Antirheumatic drugs D06694 *Clematis root; Irei-sen Drugs for external use Drugs for external use D0669

  15. Final Environmental Assessment: Proposed Laser-Firing Tank Range and Artillery Maneuver Area Arnold Air Force Base Tullahoma Tennessee (United States)


    hispidus* hairy jointgrass Significant Bromus japonicus Japanese bromegrass Significant Bromus secalinus Rye brome Significant Carduus nutans Musk ... thistle Significant Clematis ternifolia Leatherleaf clematis Significant Coronilla varia Crown vetch Significant Daucus carota Queen Anne’s-lace...eliminated. Procedures would include identification and use of areas for maneuvers that would be least likely to be disturbed by vehicular traffic, as well

  16. Final Environmental Impact Statement Second Main Operating Base KC-46A Beddown at Alternative Air National Guard Installations (United States)


    Carey’s sedge Carex careyana SE P Cattail sedge Carex typhina SE P Vasevine leather-flower Clematis viorna SE P Small yellow lady’s-slipper Cypripedium...Spreading rockcress Arabis patens SE P Northern water plantain Alisma triviale SE P Scarlet ammannia Ammannia coccinea SE P Carey’s sedge Carex careyana...SE P Cattail sedge Carex typhina SE P Vasevine leather-flower Clematis viorna SE P Small yellow lady’s-slipper Cypripedium parviflorum var

  17. GenBank blastn search result: AK059477 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059477 001-028-F01 AB117573.1 Clematis williamsii chloroplast trnQ, trnH, psbA ge...nes for tRNA-Gln, tRNA-His, PSII 32 KDa protein, partial and complete sequence.|PLN PLN 2e-35 Plus Plus ...

  18. Drug: D09111 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D09111 Formula, Drug Jashoshito Cnidium monnieri fruit [DR:D06740], Japanese root [DR:D06768], Clematis root [DR:D06694], Sophora root [DR:D06710] Traditional Chinese Medicine in Japan [BR:br08304] Formula...s Formulas for external use Formulas for external use D09111 Jashoshito PubChem: 96025791 ...

  19. Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype

    NARCIS (Netherlands)

    Woudenberg, J.H.C.; Aveskamp, M.M.; de Gruyter, J.; Spiers, A.; Crous, P.W.


    The fungal pathogen Phoma clematidina is used as a biological agent to control the invasive plant species Clematis vitalba in New Zealand. Research conducted on P. clematidina as a potential biocontrol agent against C. vitalba, led to the discovery of two perithecial-forming strains. To assess the d

  20. Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype

    NARCIS (Netherlands)

    Woudenberg, J.H.C.; Aveskamp, M.M.; de Gruyter, J.; Spiers, A.G.; P.W.Crous,


    The fungal pathogen Phoma clematidina is used as a biological agent to control the invasive plant species Clematis vitalba in New Zealand. Research conducted on P. clematidina as a potential biocontrol agent against,C. vitalba, led to the discovery of two perithecial-forming strains. To assess the d

  1. 威灵仙的化学成分及生物活性的研究进展%Research progress on chemical components and bioactivity of radix climatidis

    Institute of Scientific and Technical Information of China (English)

    阎山林; 陈丽佳; 李正翔; 张瀛化; 张骏


    Radix climatidis refers to the root and rhizoma of the one of Clematis Chinensis Osbeck,Clematis hexapstala Pall and Clematis manshurica RuPr. The chemical comPonents of radix climatidis inolve triterPenoids saPonins,flavones,lignans,couma-rins,alkaloids,sterides,organic acids,macrocyclic comPounds and Phenols. As a Chinese traditional medicine,radix climatidis is traditionaly used as analgesic,diuretic,anti - tumor and anti - inflamatic medicines,and was found recently to be active in the treatment of myocardiac infarction and atherosclerosis,Preventing the develoPment of diabetic nePhroPathy. In this PaPer,we re-viewed the advances of studies on chemical constituents and bioactivity of radix climatidis in the nearly ten years.%威灵仙为毛茛科植物威灵仙、棉团铁线莲或东北铁线莲的根及根茎,化学成分可分为挥发性成分和非挥发性两大类,主要有三萜皂苷、黄酮、木脂素、香豆素、生物碱、挥发油、甾体、有机酸、大环化合物及酚类等。威灵仙不仅具有镇痛、利尿、抗肿瘤和抗炎作用,近年来的研究还发现其可以抗心肌梗死和动脉粥样硬化,抑制糖尿病肾病的发生和发展。本文综述了近十年来威灵仙化学成分和生物活性与作用机制的研究进展。

  2. Genetic diversity of European phytoplasmas of the 16SrV taxonomic group and proposal of 'Candidatus Phytoplasma rubi'. (United States)

    Malembic-Maher, Sylvie; Salar, Pascal; Filippin, Luisa; Carle, Patricia; Angelini, Elisa; Foissac, Xavier


    In addition to the grapevine flavescence dorée phytoplasmas, other members of taxonomic group 16SrV phytoplasmas infect grapevines, alders and species of the genera Clematis and Rubus in Europe. In order to investigate which phytoplasmas constitute discrete, species-level taxa, several strains were analysed by comparing their 16S rRNA gene sequences and a set of five housekeeping genes. Whereas 16S rRNA gene sequence similarity values were >97.5 %, the proposed threshold to distinguish two 'Candidatus Phytoplasma' taxa, phylogenetic analysis of the combined sequences of the tuf, rplV-rpsC, rplF-rplR, map and uvrB-degV genetic loci showed that two discrete phylogenetic clusters could be clearly distinguished. The first cluster grouped flavescence dorée (FD) phytoplasmas, alder yellows (AldY) phytoplasmas, Clematis (CL) phytoplasmas and the Palatinate grapevine yellows (PGY) phytoplasmas. The second cluster comprised Rubus stunt (RS) phytoplasmas. In addition to the specificity of the insect vector, the Rubus stunt phytoplasma contained specific sequences in the 16S rRNA gene. Hence, the Rubus stunt phytoplasma 16S rRNA gene was sufficiently differentiated to represent a novel putative taxon: 'Candidatus Phytoplasma rubi'.

  3. Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype. (United States)

    Woudenberg, J H C; Aveskamp, M M; de Gruyter, J; Spiers, A G; Crous, P W


    The fungal pathogen Phoma clematidina is used as a biological agent to control the invasive plant species Clematis vitalba in New Zealand. Research conducted on P. clematidina as a potential biocontrol agent against C. vitalba, led to the discovery of two perithecial-forming strains. To assess the diversity of P. clematidina and to clarify the teleomorph-anamorph relationship, phylogenetic analyses of 18 P. clematidina strains, reference strains representing the Phoma sections in the Didymellaceae and strains of related species associated with Clematis were conducted. Partial sequences of the ITS1, ITS2 and 5.8S rRNA gene, the ss-tubulin gene and 28S rRNA gene were used to clarify intra- and inter-species relationships. These analyses revealed that P. clematidina resolves into three well-supported clades which appear to be linked to differences in host specificity. Based on these findings, Didymella clematidis is newly described and the descriptions of P. clematidina and D. vitalbina are amended.

  4. In situ biomonitoring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life plants: demonstration of the efficacy of emission controls in an eastern European city. (United States)

    Misík, Miroslav; Micieta, Karol; Solenská, Martina; Misíková, Katarína; Pisarcíková, Helena; Knasmüller, Siegfried


    Aim of the study was to monitor changes of genotoxic activity of urban air caused by an incinerator and a petrochemical plant in Tradescantia micronucleus (Trad-MCN) and pollen fertility assays with wild plants (Chelidonium majus, Clematis vitalba, Cichorium intybus, Linaria vulgaris, Robinia pseudoacacia). While in the first sampling period (1997-2000) significantly (on average 80%) more MN were found at the polluted site in comparison to controls from a rural area, no significant effects were observed during a later period (between 2003 and 2005). A similar pattern was observed in the pollen abortion assays in which the most pronounced effects were found in chicory and false acacia. The differences of the results obtained in the two periods can be explained by a substantial reduction of air pollution by use of new technologies. In particular the decrease of SO(2) emissions may account for the effects seen in the present study.


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    N. V. Gerasimyuk


    Full Text Available The spring flora was analyzed on six cemeteries in Odessa, such cemeteries as Vtoroe xristianskoe, Troickoe, Tairovskoe, Trete evrejskoe, Oficerskoe (Dmitrievskое and Severnoe. There were found and identified 235 species of plants, which belong to 186 genera and 67 families. There was taken a taxonomic analysis of flora of the cemeteries also of spreding of plants by ekobiomorphs, the analysis of hronotyp and origin. The proportion of the flora on the cemeteries is 1:2,8:3,5. The dominant family of the flora of Odessa’s cemeteries is Asteraceae. There are other leading families such as Rosaceae, Fabaceae, Poaceae, Brassicaceae, Lamiaceae. Herbs and trees are dominant among the life forms. Mesophytes and kseromezophytes are in the majority among hihromorphs. Heliophytes are on the first place by adapting to the light. Our results showed that the adventitious plants occupy up to 44% of all amount of plants at the cemeteries in Odessa. Kenophytes is a dominant group among them. Floragenetics analysis revealed the dominance of the plants from Asia. There have been allocated plants that were met at all six cemeteries independently of the location religious and age characteristics of the cemetery. "Core" of the flora of Odessa’s cemetery have made weed Acer negundo L., Ballota nigra L. and decorative Hedera helix L., Centaurea dealbata Willd., Buxus sempervirens L., Convallaria majalis L., Sedum kamtschaticum Fisch., Thuja occidentalis L., Hemerocalis fulva (L. L. There were found 4 species of plants that belong to the rare and endangered plants of Odessa’s region: Convallaria majalis L., Hyacinthella leucophaea (K. Koch Schur, Clematis integrifolia L., Paeonia tenuifolia L. Moreover Convallaria majalis L. grows on all six investigated cemeteries. Also two species: Hyacinthella leucophaea (K. Koch Schur and Clematis Keywords: cemeteries, Odessa, flora, plants, ekobiomorphs.

  6. Extraction and Antibacterial Properties of Flavonoids from Wild Herbs%几种野菜中黄酮的提取及抑菌作用研究

    Institute of Scientific and Technical Information of China (English)

    丁存宝; 李桂秋; 刘海燕; 贾长虹; 张学祥; 常立新


    Flavonoid compound extensively existed to a second-class metabolism outcome of the polyphenol in plant had a lot of potential medicinal value. Antibacterial activity of flavonoid was the hot spots of study. The antibacterial activity of flavonoid from several wild herbs was tested by using the bacteria Bacillus subtilis, Escherichia coli, Salmonella typhi, fungi Penicillium, Saccharomyces cerevisiae as the tested strains. All the samples collected to isolate flavonoids from Lespedeza and Clematis Aethusifolia. The flavonoid extracts were diluted with double dilution method. Antibacterial activity of flavonoid was measured by taking inhibition zone method. The results showed that the antibacterial activity of flavonoid from several wild herbs: Escherichia coli〉Bacillus subtilis〉Salmonella typhi 〉Accharomyces〉Penicillium glaucum. The antibacterial activity was correspondent with the extract flavonoid's concentration. The MIC of Lespedeza and Clematis were 332.6μL/mL and 441.5 μL/mL, respectively. The antibacterial activity of flavonoids from Clematis was superior to Lespedeza.%黄酮类化合物是广泛存在于自然界中的多酚类植物次级代谢产物,具有许多潜在的药用价值和生物活性,其中抑菌活性是研究热点之一。以枯草芽孢杆菌(Bacillus subtilis)、大肠杆菌(Escherichia coli)、伤寒杆菌(Salmonella typhi)和青霉菌(Penicillium glaucum)、酿酒酵母(Accharomyces)为供试菌研究了几种野菜中黄酮提取物的抑菌作用。首先从芹叶铁线莲和胡枝子中分离提取黄酮提取液,用二倍稀释法制成不同浓度的黄酮溶液。再采取圆滤纸片法进行抑菌试验,测量出不同菌种和不同黄酮浓度下的抑菌圈直径。从试验结果得出野菜黄酮对供试菌的抑菌效果依次为:大肠杆菌〉枯草杆菌〉伤寒杆菌〉酿酒酵母〉青霉菌。结果表明抑菌活性与对应提取物中总黄酮有关,其含量直接影

  7. Preliminary results of studies on the distribution of invasive alien vascular plant species occurring in semi-natural and natural habitats in NW Poland

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    Popiela Agnieszka


    Full Text Available In Western Pomerania, as in other areas of Europe, alien species play an increasingly important role. In particular, invasive plants tend to spread rapidly and in large numbers which may reduce diversity of native species, leading to the phenomenon of “trivialisation of flora”, and transform ecosystems. The list of invasive species (32 taxa includes alien species occurring throughout Western Pomerania, and penetrating natural or semi-natural habitats. The second group consists of potentially invasive species (23 taxa, i.e. those distributed across the area under study and tending to increase the number of their localities in semi-natural and natural habitats, taxa invasive only locally, as well as species with missing data, which does not currently allow including them into the first group. Invasive weeds, as well as some epecophytes and archaeophytes occurring only on anthropogenic sites and tending to spread, were not taken into account. Among hemiagriophytes, the most common and troublesome ones are: Conyza canadensis, Erigeron annuus, Lolium multiflorum, Lupinus polyphyllus, Solidago canadensis, S. gigantea. Among holoagriophytes, i.e. the taxa which received the highest naturalisation status, very expansive species, successful in land colonisation, like Acer negundo, Bidens frondosa, B. connata, Clematis vitalba, Elodea canadensis, Epilobium ciliatum, Heracleum sosnowskyi, Impatiens glandulifera, I. parviflora, Padus serotina, Quercus rubra and Robinia pseudoacacia, should be given particular attention. Among the invasive and potentially invasive species, most taxa penetrate plant communities of the Artemisietea and Molinio-Arrhenatheretea class, followed by Querco-Fagetea, Vaccinio-Piceetea, Stellarietea mediae, Salicetea purpurae and Koelerio-Corynophoretea. The number of invasive species is twice as high when compared to the situation of these species in Poland; on the contrary, the number of species inhabiting anthropogenic, semi

  8. Streamflow and Soil Moisture of Agroforestry and Grass Watersheds in Hilly Area

    Institute of Scientific and Technical Information of China (English)

    LIU Gang-Cai; TIAN Guang-Long; SHU Dong-Cai; LIN San-Yi; LIU Shu-Zhen


    A study was conducted in a hilly area of Sichuan Province,Southwestern China, to compare the streamflow and soil moisture in two upland watersheds with different land use patterns. One was an agroforestry watershed, which consisted mainly of trees with alder (Alnus cremastogyne Burkill) and cypress (Cupressus funebris Endl.) planted in belts or strips with a coverage of about 46%, and the other was a grassland primarily composed of lalang grass (Imperata cylindrica var. major (Nees) C. E. Hubb.), filamentary clematis (Clematis filamentosa Dunn) and common eulaliopsis (Eulaliopsis binata (Retz.) C. E. Hubb) with a coverage of about 44%. Streamflow measurement with a hydrograph established at the watershed outlet showed that the average annual streamflow per 100 mm rainfall from 1983 to 1992 was 0.36 and 1.08 L s-1 km-2 for the agroforestry watershed and the grass watershed, respectively. This showed that the streamflow of the agroforestry watershed was reduced by 67% when compared to that of the grass watershed. The peak average monthly streamflow in the agroforestry watershed was over 5 times lower than that of the grass watershed and lagged by one month. In addition, the peak streamflow during a typical rainfall event of 38.3 mm in August 1986 was 37% lower in the agroforestry watershed than in the grass watershed. Results of the moisture contents of the soil samples from 3 slope locations (upper, middle and lower slopes) indicated that the agroforestry watershed maintained generally higher soil moisture contents than the grass watershed within 0-20 and 20-80 cm soil depths for the upper slope, especially for the period from May through July. For the other (middle and lower) slopes, soil moisture contents within 20-80 cm depth in the agroforestry watershed was generally lower than those in the grass watershed, particularly in September, revealing that water consumption by trees took place mainly below the plow layer. Therefore, agroforestry land use types might

  9. Avaliação dos efeitos centrais dos florais de Bach em camundongos através de modelos farmacológicos específicos Evaluation of central effects of Bach Flowers Remedies in mice using specific pharmacological models

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    Márcia M. De-Souza


    Full Text Available Os Remédios Florais de Bach (RFB, constituem um método alternativo de tratamento usado largamente na terapêutica de várias patologias em muitos países do mundo. Os RFB são reconhecidos como tratamento natural pela OMS desde 1956. Embora o mecanismo de ação dos RFB ainda não tenha sido elucidado, eles vêm sendo indicados para o tratamento de várias doenças neuropsiquiátricas. O objetivo do presente trabalho foi detectar possíveis efeitos centrais dos RFB em modelos farmacológicos utilizados na pesquisa de substâncias com efeitos ansiolíticos, hipnóticos, antidepressivos e neurolépticos. Para tanto, camundongos receberam um tratamento agudo via oral (0,45 mL 1 hora antes dos testes. Os resultados mostraram que os florais Gorse e, em conjunto, White chestnut, Agrymony e Vervain exibiram perfis antidepressivo e hipnótico, respectivamente. No modelo de ansiedade foi detectado efeito ansiolítico do floral Agrymony. Entretanto, não foram observados efeitos neurolépticos do floral Clematis. Os resultados nos levam a sugerir que os efeitos centrais dos florais avaliados podem ser parcialmente detectados através de modelos farmacológicos utilizados na pesquisa de agentes psicotrópicos.The Bach Flowers Remedies (BFR's are worldwide used as an alternative therapeutical approach for several pathologies, being considered by WHO as natural therapy since 1956. Despite the unknown mechanism of action, the BFR's have been widely used on treatment of several neuropsychiatry diseases. Based on pharmacological models used to detect ansiolitic, antidepressant, hypnotic and neuroleptyc effects of different substances, the aim of this work was to evaluate possible central effects of the BFR's. For this purpose, albino mice received BFR's treatment (0.45 mL by oral route 1 hour prior to each test. The results revealed that the Gorse flower alone and a mix of White chestnut, Agrymony and Vervain showed antidepressant and hypnotic effects

  10. Do the Golden-winged Warbler and Blue-winged Warbler Exhibit Species-specific Differences in their Breeding Habitat Use?

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    David S. Maehr


    Full Text Available We compared habitat features of Golden-winged Warbler (Vermivora chrysoptera territories in the presence and absence of the Blue-winged Warbler (V. cyanoptera on reclaimed coal mines in southeastern Kentucky, USA. Our objective was to determine whether there are species specific differences in habitat that can be manipulated to encourage population persistence of the Golden-winged Warbler. When compared with Blue-winged Warblers, Golden-winged Warblers established territories at higher elevations and with greater percentages of grass and canopy cover. Mean territory size (minimum convex polygon was 1.3 ha (se = 0.1 for Golden-winged Warbler in absence of Blue-winged Warbler, 1.7 ha (se = 0.3 for Golden-winged Warbler coexisting with Blue-winged Warbler, and 2.1 ha (se = 0.3 for Blue-winged Warbler. Territory overlap occurred within and between species (18 of n = 73 territories, 24.7%. All Golden-winged and Blue-winged Warblers established territories that included an edge between reclaimed mine land and mature forest, as opposed to establishing territories in open grassland/shrubland habitat. The mean distance territories extended from a forest edge was 28.0 m (se = 3.8 for Golden-winged Warbler in absence of Blue-winged Warbler, 44.7 m (se = 5.7 for Golden-winged Warbler coexisting with Blue-winged Warbler, and 33.1 m (se = 6.1 for Blue-winged Warbler. Neither territory size nor distances to forest edges differed significantly between Golden-winged Warbler in presence or absence of Blue-winged Warbler. According to Monte Carlo analyses, orchardgrass (Dactylis glomerata, green ash (Fraxinus pennsylvanica seedlings and saplings, and black locust (Robinia pseudoacacia saplings were indicative of sites with only Golden-winged Warblers. Sericea lespedeza, goldenrod (Solidago spp., clematis vine (Clematis spp., and blackberry (Rubus spp. were indicative of sites where both species occurred. Our findings complement recent genetic studies and add

  11. Development and validation of a chromatographic method for determining Clematichinenoside AR and related impurities

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    Zhou Yang


    Full Text Available Abstract Background Clematichinenoside AR is a promising lead compound for the treatment of rheumatoid arthritis. A systematic research for the related impurities in AR bulk samples is still lacking. For the safe use of this natural product in future clinical practice, the structure and content of each constituent, including the main ingredient as well as the impurities in AR bulk sample must be characterized in detail. Results A simple and stability indicating RP-HPLC method was developed and validated for determining the purity of clematichinenoside AR (AR, a natural product from the roots of Clematis manshurica Rupr. (Ranunculaceae with the potential of treating rheumatoid arthritis. Five impurities were characterized, and impurity 2 (Clematomandshurica saponin F is a new triterpenoid saponin isolated from this product. Optimum separation for clematichinenoside AR and five related impurities was carried out on an Agilent octadecylsilane bonded silica gel column (TC-C18, 4.6 mm ×150 mm, 5 μm using a gradient HPLC method. The validation results showed good sensitivity, specificity, linearity(r2>0.9992 precision(RSD Conclusion Five related impurities of clematichinenoside AR were characterized, including a new triterpenoid saponins firstly found in clematichinenoside AR bulk samples. In the simple chromatographic method for determining clematichinenoside AR and its related impurities in bulk samples, the correction factor was better for the quality control in the relative stable concentrations.

  12. Antisporulant Activity of Watery Extracts of Plants against Sclerospora graminicola Causing Downy Mildew Disease of Pearl Millet

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    S. A. Deepak


    Full Text Available Watery extracts of forty plant species commonly growing in across India have been screened for antisporulant activity against Sclerospora graminicola (Sacc. Schroet., the causative agent of pearl millet downy mildew. The collection represented 38 genera of 30 families. The extracts of thirteen species did not show any effect, whereas the activity of extracts of Allium sativum, Clematis gouriana, Evolvulus alsinoides, Mimusops elengi, Parthenium hysterophorus, Piper nigrum and Tagetes erecta were commensurable to that of marketed botanical fungicides and Mikal 70 wp. The crude extracts of 12 species (Agave americana, Aloe vera, Artemisia parviflora, Citrus limon, Citrus sinensis, Eucalyptus globosus, Euphorbia hirta, Leucas aspera, Murraya koenigi, Ocimum sanctum, Santalum album and Zingiber offinale completely inhibited the zoosprorangium formation while in the case of remaining 8 plants the crude extracts reduced only partially the sporulation. The antisporulant activity of commercialised Azadirachta preparation (Nutri-Neem was more pronounced than that of Reynutria based one (Milsana and Sabadilla (veratrin, however, these botanical preparations held off synthetic fungicides and the most active watery extracts.

  13. Ozone air pollution and foliar injury development on native plants of Switzerland

    Energy Technology Data Exchange (ETDEWEB)

    Novak, Kristopher; Skelly, John M.; Schaub, Marcus; Kraeuchi, Norbert; Hug, Christian; Landolt, Werner; Bleuler, Peter


    Visible ozone-induced foliar injury on native forest species of Switzerland was identified and confirmed under ambient OTC-conditions and related to the current European AOT40 standard. - The objectives of this study were to examine the foliar sensitivity to ozone exposure of 12 tree, shrub, and herbaceous species native to southern Switzerland and determine the seasonal cumulative ozone exposures required to induce visible foliar injury. The study was conducted from the beginning of May through the end of August during 2000 and 2001 using an open-top chamber research facility located within the Lattecaldo Cantonal Forest Nursery in Canton Ticino, southern Switzerland (600 m asl). Plants were examined daily and dates of initial foliar injury were recorded in order to determine the cumulative AOT40 ppb h ozone exposure required to cause visible foliar injury. Plant responses to ozone varied significantly among species; 11 species exhibited visible symptoms typical of exposures to ambient ozone. The symptomatic species (from most to least sensitive) were Populus nigra, Viburnum lantana, Salix alba, Crataegus monogyna, Viburnum opulus, Tilia platyphyllos, Cornus alba, Prunus avium, Fraxinus excelsior, Ribes alpinum, and Tilia cordata; Clematis spp. did not show foliar symptoms. Of the 11 symptomatic species, five showed initial injury below the critical level AOT40 10 ppmh O{sub 3} in the 2001 season.

  14. A cross-cultural study: anti-inflammatory activity of Australian and Chinese plants. (United States)

    Li, Rachel W; Myers, Stephen P; Leach, David N; Lin, G David; Leach, Greg


    In this study, in vitro inhibitory effects of 33 ethanol extracts obtained from 24 plant species (representing 11 different families) on cyclooxygenase-1 (COX-1) were evaluated. The plant materials selected for this study have been used in aboriginal medicine in Australia and traditional medicine in China for the treatment of various diseases that are considered as inflammation in nature, e.g. asthma, arthritis, rheumatism, fever, edema, infections, snakebite and related inflammatory diseases. All of the selected plants, with one exception, showed inhibitory activity against COX-1, which supports their traditional uses. The most potent COX-1 inhibition were observed from the extracts of Acacia ancistrocarpa leaves (IC(50)=23 microg/ml). Ficus racemosa bark, Clematis pickeringii stem, Acacia adsurgens leaves, Tinospora smilacina stem and Morinda citrifolia fruit powder exhibited inhibition of COX-1 with the IC(50) of 100, 141, 144, 158 and 163 microg/ml, respectively. Aspirin and indomethacin used as the reference COX-1 inhibitors in this study inhibited COX-1 with IC(50) of 241 and 1.2 microg/ml, respectively. The findings of this study may explain at least in part why these plants have been traditionally used for the treatment of inflammatory conditions in Australian aboriginal medicine and traditional Chinese medicine.

  15. An ethnoveterinary study of medicinal plants in treatment of diseases and syndromes of herd dog in southern regions of Ilam province, Iran. (United States)

    Bahmani, Mahmoud; Eftekhari, Zohre


    This paper describes a selection of the ethnoveterinary medicines used for herd dogs in the southern regions of Ilam province, Iran. Traditional botanical medicine is the primary mode of healthcare for most of the rural population in Ilam province. In this study, a questionnaire was distributed among 45 residential areas in 22 rural zones of the southern areas of Ilam province. The objective of this study was the recognition of natural medicinal methods using medicinal plants, and the classification of ethnoveterinary applications and collection of domestic science. Twenty-two medicinal plants from 16 families were identified. The main application of these plants was for the detection and treatment of digestive disorders using Citrullus colocynthis, Aristolochia clematis, Scrophularia deserti, Quercus brantii, Ceracus microcarpa, Echium strigosa, Pistacia atlantica, and Pistacia khinjuk which have been applied using Euphurbia graminifolius, Peganum harmala, Salsola rigida, Artemisia herba-alba, Amygdalus arabica, jolbak of salt water, Peganum harmala L., and Nicotina tabacum for external and internal parasite disorders. S. deserti for ophthalmic disorders, and P. atlantica, P. khinjuk, and Q. brantii for respiratory disorders were applied. The present study confirmed the traditional medical effects of some plants and revealed the unique medical effects of other plants, which if recognized could be useful in the creation of new ideas and increasing knowledge for the modern pharmaceutical industry. Since very few clinical trials have been conducted on plants native to Ilam province, it is necessary that more research be conducted to ensure that labeled and standardized products are introduced for human consumption.

  16. Screening of radical scavenging activity and polyphenol content of Bulgarian plant species

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    Milena Nikolova


    Full Text Available Background: Discovery of new plant species with antioxidant properties is a priority of many research teams. Most of the species included in this study are unstudied for antioxidant properties, but they are taxonomically related to reference plants with well-documented antioxidant activity. Materials and Methods: Free radical scavenging activity of plant extracts was evaluated using a 1,1-diphenyl-2-picrylhydrazyl (DPPH assay. An aluminum chloride colorimetric method was used for flavonoid determination. The amount of phenolic compounds in the extracts was estimated by using the Folin-Ciocalteu reagent. Results: As a result of screening, it was found that the significant antioxidant properties possess several unstudied until now plant species (Veronica bellidioides L., V. kellereri Deg. et Urm, V. vindobonensis (M. Fisher M. Fisher, V. beccabunga L., V. rhodopaea L., V. austriaca (Velen. Degen., Clinopodium vulgare L., Stachys recta L., Clematis vitalba L., and Xeranthemum annum L.. The antioxidant potential of the new species is comparable to that of reference medicinal plants. Conclusions: The existing data presented here provide new information for antioxidant potential of plant species that have not been traditionally used as medicinal plants.

  17. In vitro evaluering van die effektiwiteit van vyf plante wat tradisioneel teen seksueel oordraagbare siektes gebruik word

    Directory of Open Access Journals (Sweden)

    H. Swart


    Full Text Available Tot soveel as 60% van die Suid-Afrikaanse bevolking raadpleeg tradisionele genesers vir siektetoestande, insluitende seksueel oordraagbare siektes (SOS. Medisinale plante speel ’n belangrike rol in die behandeling van SOS deur tradisionele genesers, maar sonder enige wetenskaplike bewyse van effektiwiteit. Vir hierdie studie is 5 plante vanuit die literatuur gekies op grond van hul gebruik deur tradisionele genesers vir die behandeling van SOS. Siftingstoetse vir die bepaling van aktiwiteit teen bakterieë en teen SOS is op die plante uitgevoer. Die plante was Clematis brachiata, Elephantorrhiza elephantina, Lepidium bonariense, Ranunculus multifidus en Typha capensis. Siftingstoetse is uitgevoer met die organismes Candida albicans, Neisseria gonorrhoeae en Haemophilus ducreyi wat met SOS geassosieer word, terwyl Bacillus subtilus, Micrococcus luteus, Staphylococcus aureus, Enterobacter sp., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa en Aspergillus niger ook in die siftingstoetse ingesluit is. Slegs Lepidium bonariense het aktiwiteit teen die gebruikte organismes getoon. Dit is dus duidelik dat daar ’n behoefte bestaan om die effektiwiteit van medisinale plante wetenskaplik te bepaal en te kommunikeer.

  18. Antifungal activity of plant extracts against dermatophytes. (United States)

    Ali-Shtayeh, M S; Abu Ghdeib, S I


    The aqueous extracts (15 micrograms ml-1 medium) of 22 plants used in folkloric medicine in Palestine were investigated for their antifungal activity and minimum inhibitory concentrations (MICs) against nine isolates of Microsporum canis, Trichophyton mentagrophytes and Trichophyton violaceum. The extract of the different plant species reduced colony growth of the three dermatophytes by 36 to 100% compared with the control treatment. Antimycotic activity of the extract against the three dermatophytes varied significantly (P Inula viscosa, J. regia and P. lentiscus against T. mentagrophytes; and Asphodelus luteus, A. arvensis, C. spinosa, Clematis cirrhosa, I. viscosa, J. regia, P. lentiscus, Plumbago europea, Ruscus aculeatus, Retema raetam and Salvia fruticosa against T. violaceum. The MICs of these most active plants ranged from 0.6 to 40 micrograms ml-1. The three dermatophytes differed significantly with regard to their susceptibility to plant extracts. Trichophyton violaceum was the most susceptible being completely inhibited by 50% of the extracts followed by M. canis and T. mentagrophytes which were completely inhibited by only 23 and 14% of the extracts, respectively.

  19. In situ biomonitoring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life plants: Demonstration of the efficacy of emission controls in an eastern European city

    Energy Technology Data Exchange (ETDEWEB)

    Misik, Miroslav [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Micieta, Karol [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Solenska, Martina [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Misikova, Katarina [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Pisarcikova, Helena [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Knasmueller, Siegfried [Institute of Cancer Research, Department of Inner Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna (Austria)]. E-mail:


    Aim of the study was to monitor changes of genotoxic activity of urban air caused by an incinerator and a petrochemical plant in Tradescantia micronucleus (Trad-MCN) and pollen fertility assays with wild plants (Chelidonium majus, Clematis vitalba, Cichorium intybus, Linaria vulgaris, Robinia pseudoacacia). While in the first sampling period (1997-2000) significantly (on average 80%) more MN were found at the polluted site in comparison to controls from a rural area, no significant effects were observed during a later period (between 2003 and 2005). A similar pattern was observed in the pollen abortion assays in which the most pronounced effects were found in chicory and false acacia. The differences of the results obtained in the two periods can be explained by a substantial reduction of air pollution by use of new technologies. In particular the decrease of SO{sub 2} emissions may account for the effects seen in the present study. - Air pollution caused by industrial emissions induced micronuclei in Tradescantia and increased pollen abortion in wild plant species.

  20. 间歇期及慢性期痛风中医方药的文献研究%Literature Research of Traditional Chinese Medical Prescriptions of Gout in Intermission and Chronic Phase

    Institute of Scientific and Technical Information of China (English)

    李海昌; 温成平; 谢志军; 韩春雯; 汪梅姣


    目的:探求痛风间歇期及慢性期的方药特点,为中医临床治疗奠定基础.方法:采用计算机检索和人工检索相结合的方法,对1997年1月-2011年6月国内期刊中相关文献进行回顾性总结.结果:检索出符合要求的文献85[1-82]篇,共涉及痛风间歇期、慢性期及慢性关节炎期患者累计4791例,主要中药(前15味)使用频率结果分别是:牛膝(36.11%)、桑寄生(31.48%)、薏苡仁(30.56%)、土茯苓(26.85%)、苍术(25.93%)、白术(25.93%)、白芥子(25.00%)、当归(24.07%)、黄柏(23.15%)、黄芪(22.22%)、防己(22.22%)、草薢(20.37%)、威灵仙(20.37%)、赤芍(18.52%)、秦艽(16.67%).结论:牛膝、桑寄生、苍白术、薏苡仁及黄芪健脾益肾,泄浊祛湿治病之本;当归、牛膝、苍白术、赤芍、黄柏、白芥子及威灵仙活血化瘀通络,燥湿化痰通痹治病之标.%Objective: To provide a basis for clinical treatment of traditional Chinese medicine ( TCM) by seeking the TCM prescriptions of gout in intermission and chronic phase. Methods: Domestic relevant literatures were summarized retrospectively based on the interaction by computer search and artificial retrieval from January 1997 to June 2011. Results-. Totally 85 literatures were retrieved to meet the requirements, including 4791 cases of intermission, chronic phase and chronic gouty arthritis. The distributions of major TCM prescriptions (the top 15)were achyranthes root(36. 11% ) ,mis-tletoe( 31. 48% ) , coix seed ( 30. 56% ), smilax Glabra ( 26. 85% ), atractylodes ( 25.93% ) , rhizoma atractylodis (25.93%) ,white mustard seed(25.00% ) ,angelica sinensis(24. 07% ) ,cortex phellodendri(23. 15%) ,radix astragali (22.22% ) ,tetrandra root(22.22%) ,yam rhizome(20. 37% ),clematis root(20. 37% ) ,red peony root(18.52%) ,gen-tiana macrophylla ( 16. 67% ). Conclusions :It is the fundamental treatment of gout by invigorating spleen and nourishing kidney therapy for reducing

  1. Diversity, Distribution and Prioritization of Fodder Species for Conservation in Kullu District, Northwestern Himalaya, India

    Institute of Scientific and Technical Information of China (English)


    In the Indian Himalayan Region predominantly rural in character, livestock is one of the main sources of livelihood and integral part of the economy. Livestock mostly rely on fodder from wild.The diversity, distribution, utilization pattern, nativity,endemism, rarity, seasonality of availability, nutritive values, perceived economic values and pressure use index of livestock have not been studied. The present study attempts to enumerate 150 species of fodder representing trees (51 spp.), shrubs (54 spp.) and herbs (45 spp.). Poaceae (19 spp.) and Fabaceae (13 spp.) amongst families and Salix (6 spp.), Ficus,Clematis, and Desmodium (5 spp., each) amongst genera are rich in species. Maximum species were found in the 1801 ~ 2600 m zone, and the remaining two zones showed relatively low diversity. Out of the 150 species, 109 are used in summer, 5 winter and 36 throughout year. During rainy season, mostly grasses are used as fodder. Only 83 species are native to the Himalayan region, one species, Strobilanthus atropuroureus is endemic and 35 species are near endemic. The nutritive values of the fodder species were reviewed, and economic values and status of the species were also assessed. The pressure use index of the species was calculated on the basis of cumulative values of the utilization pattern,altitudinal distribution, availability, status, nativity and endemism. Amongst the species, Grewia oppositifoilia, Morus serrata, Indigofera heterantha,Quercus leucotrichphora, Ulmus villosa, U.wallichiana and Aesculus indica showed highest PUI indicating high preference and pressure. Season wise prioritization of the species for different altitudinal ones has been done. Appropriate strategy and action plan have been suggested for the conservation and management of fodder species.

  2. Nature Reserve wild plants ornamental value evaluation in Tumuji%图牧吉自然保护区野生植物观赏价值评价

    Institute of Scientific and Technical Information of China (English)

    潘国富; 杜广明; 朱琳; 宋敏超


    The ornamental value of wild plant resources in Tumuji Nature Reserve was evaluated by analytic hierarchy process (AHP) to establish the foundation for exploitation and utilization of wild plant resources in the premise of protection. The results showed that, the 9 kinds of wild plant had higher comprehensive evaluation value such as PotentiUa anserina L., Thymus quinquecostatus vat. asiat/cus Kitag., Lilium pumilum DC, Portulaca grandiflora L., Thalictrum petaloideum L., Clematis hexapetala Pall., Medicago falcata L., Leontoodium leonptopodioides (Willd.) Beauv. and Bupleurum scorzonerifolium Willd.which could be considered as the introduction and application of wild native plant species in peripheral region and could be protectively developed and utilized.%采用层次分析法(AHP)评价了图牧吉自然保护区野生植物资源的观赏价值,为在保护的前提下充分开发利用该保护区野生植物资源奠定了基础。结果表明,鹅绒萎陵菜、百里香、细叶百合、太阳花、瓣蕊唐松草、棉团铁线莲、野苜蓿、火绒草、狭叶柴胡等9种野生植物综合评价值较高,是周边地区可以考虑引种应用的乡土野生植物种类,可进行保护性开发和利用。

  3. Ethnopharmacological survey of wild medicinal plants in Showbak, Jordan. (United States)

    Al-Qura'n, S


    Two main research questions are framing this investigation: (1) the main taxa of the medicinal importance value altered the Showbak forest stand and species composition? (2) The most safe species and what are the toxic ones (unsafe). These two research questions are the vital ones to draw a clear image about the wild medicinal plants of this investigated area of Showbak region in Jordan. 79 wild medicinal plant species were investigated in this study which are used in traditional medication for the treatment of various diseases. Most of the locals interviewed dealt with well-known safe medicinal plants such as Aaronsohnia factorovskyi Warb. et Eig., Achillea santolina L., Adiantum capillus-veneris L., Artemisia herba-alba L., Ceratonia siliqua L., Clematis recta L., Herniaria hirsuta L., Malva neglecta Wallr., Rosmarinus officinalis L., Ruta chalepensis L., Salvia triloba L., Sarcopoterium spinosa (L.) Spach., Thymbra capitata (L.) Hof, and Urginea maritima Barker. Many of the wild medicinal plants investigated were toxic and needed to be practiced by practitioners and herbalists rather than the local healers. These plants include Calotropis procera Willd R.Br., Citrullus colocynthis (L.) Sch., Datura stramonium L., Digitalis purpurea L., Ecballium elaterium (L.) A.Rich., Euphorbia helioscopia L., Euphorbia tinctoria Boiss., Glaucium corniculatum (L.) Curt., Hyoscyamus aureus L., Mandragora officinarum L., Nerium oleander L., Ricinus communis L., Solanum nigrum L., Withania somnifera (L.) Dunel. The conservation of medicinal plants and natural resources is becoming increasingly important, so this research is trying to collect information from local population concerning the use of medicinal plants in Showbak; identify the most important specie; determine the relative importance value of the species and calculate the informant consensus factor (ICF) for the medicinal plants. Obtaining results is relied on the interviewee's personal information and the medicinal use

  4. Clematichinenoside inhibits VCAM-1 and ICAM-1 expression in TNF-α-treated endothelial cells via NADPH oxidase-dependent IκB kinase/NF-κB pathway. (United States)

    Yan, Simin; Zhang, Xu; Zheng, Haili; Hu, Danhong; Zhang, Yongtian; Guan, Qinghua; Liu, Lifang; Ding, Qilong; Li, Yunman


    Proinflammatory cytokine TNF-α-induced adhesion of leukocytes to endothelial cells plays a critical role in the early stage of atherosclerosis. Oxidative stress and redox-sensitive transcription factors are implicated in the process. Thus, compounds that mediate intracellular redox status and regulate transcription factors are of great therapeutic interest. Clematichinenoside (AR), a triterpene saponin isolated from the root of Clematis chinensis Osbeck, was previously demonstrated to have anti-inflammatory and antioxidative properties. However, little is known about the exact mechanism underlying these actions. Thus we performed a detailed study on its effect on leukocytes-endothelial cells adhesion with TNF-α-stimulated human umbilical vein endothelial cells (HUVECs) and cell-free systems. First, we found that AR reduced TNF-α-induced VCAM-1 and ICAM-1 expression and their promoter activity, inhibited translocation of p65 and phosphorylation of IκBα, suppressed IκB kinase-β (IKK-β) activity, lowered O2(∙-) and H2O2 levels, tackled p47(phox) translocation, and decreased NOX4 NADPH oxidase expression. Second, we showed that AR exhibited no direct free radical scavenging ability in cell-free systems at concentrations that were used in intact cells. Besides, AR had no direct effect on the activity of IKK-β that was extracted from TNF-α-stimulated HUVECs. We also found that p47 translocation, NOX4 expression, and reactive oxygen species (ROS) levels were up-regulated before IκB phosphorylation in TNF-α-induced HUVECs. Moreover, TNF-α-enhanced IKK-β activity was also inhibited by (polyethylene glycol) PEG-catalase, N-acetylcysteine (NAC), and vitamin E. In conclusion, these results suggest that AR reduces VCAM-1 and ICAM-1 expression through NADPH oxidase-dependent IKK/NF-κB pathways in TNF-α-induced HUVECs, which finally suppress monocyte-HUVECs adhesion. This compound is potentially beneficial for early-stage atherosclerosis.

  5. Pharmacologic evalution and application of some anti-cancer plants from Changbai Mountain%长白山若干抗癌植物的药理评价及应用

    Institute of Scientific and Technical Information of China (English)

    金洙哲; 李相来; 李钟一


    从1956年至2000年在长白山采集了抗癌植物,有61科,129属,162属.在本文提出其中10种,讨论其药理评价及临床应用,种类如F:(1)核桃楸Juglans nandshurica Maxim.胡桃科;(2)北鸟头Aconitum kusnezoffi Reichb.毛莨科;(3)黄耆Astragalus membranaceus(Fisch)Bunge.豆科;(4)棉团铁线莲Clematis hexapetala Pall.毛莨科;(5)龙葵Solanum nigrum Linnaeus.茄科;(6)龙牙草Agrimonia pilosa Ledeb.蔷薇科.(7)东北天南星Arisaema amurense Maxin.天南星科;(8)毛莨Ranunculus japonicus Thunberg.毛莨科.(9)白屈菜Chelidonium majus Linnaeus.罂粟科.蝙蝠葛Menispermum dauricum Dc.防已科.%Various kinds of plants were collected from the Changbai Mountain from 1956 to 2000. They were identified and classified into anti-cancer plants. According to the results, they were composed of 61 families, 129 genera and 162 species. Among them, 10 species of anti-cancer plants were discussed on their pharmacologic evalution and application, the plants were (1)J uglans mandshurica Maxim; (2) Aconitum kusnezoffi Reichb; ( 3 ) Astragalus membranaceus (Fisch) Bunge; (4) Clemat is hexapetala Pall; ( 5 ) Solanum nigrum Linnaeus; ( 6 ) Agrimonia pilosa Ledeb. (7) Arisaema amurense Maxim. (8) Ranunculus japonicus Thunberg.; ( 9 ) Chelidonium majus Linnaeus. Menispermumdauricum Dc.

  6. Drug metabolism and pharmacokinetic diversity of ranunculaceae medicinal compounds. (United States)

    Hao, Da-Cheng; Ge, Guang-Bo; Xiao, Pei-Gen; Wang, Ping; Yang, Ling


    The wide-reaching distributed angiosperm family Ranunculaceae has approximately 2200 species in around 60 genera. Chemical components of this family include several representative groups: benzylisoquinoline alkaloid (BIA), ranunculin, triterpenoid saponin and diterpene alkaloid, etc. Their extensive clinical utility has been validated by traditional uses of thousands of years and current evidence-based medicine studies. Drug metabolism and pharmacokinetic (DMPK) studies of plant-based natural products are an indispensable part of comprehensive medicinal plant exploration, which could facilitate conservation and sustainable utilization of Ranunculaceae pharmaceutical resources, as well as new chemical entity development with improved DMPK parameters. However, DMPK characteristics of Ranunculaceaederived medicinal compounds have not been summarized. Black cohosh (Cimicifuga) and goldenseal (Hydrastis) raise concerns of herbdrug interaction. DMPK studies of other Ranunculaceae genera, e.g., Nigella, Delphinium, Aconitum, Trollius, and Coptis, are also rapidly increasing and becoming more and more clinically relevant. In this contribution, we highlight the up-to-date awareness, as well as the challenges around the DMPK-related issues in optimization of drug development and clinical practice of Ranunculaceae compounds. Herb-herb interaction of Ranunculaceae herb-containing traditional Chinese medicine (TCM) formula could significantly influence the in vivo pharmacokinetic behavior of compounds thereof, which may partially explain the complicated therapeutic mechanism of TCM formula. Although progress has been made on revealing the absorption, distribution, metabolism, excretion and toxicity (ADME/T) of Ranunculaceae compounds, there is a lack of DMPK studies of traditional medicinal genera Aquilegia, Thalictrum and Clematis. Fluorescent probe compounds could be promising substrate, inhibitor and/or inducer in future DMPK studies of Ranunculaceae compounds. A better

  7. 25 Years of Natural Product R&D with New South Wales Agriculture

    Directory of Open Access Journals (Sweden)

    I. Southwell


    Full Text Available Following recent NSW Government restructuring, the Department of Agriculture now exists in a composite form along with Forestry, Fisheries and Minerals in the new NSW Department of Primary Industries. This paper outlines some of the highlights of secondary metabolite R&D accomplished in the 25 years since the essential oil research unit was transferred from the Museum of Applied Arts & Sciences, Sydney to NSW Agriculture’s Wollongbar Agricultural Institute on the NSW north coast. The essential oil survey was continued, typing the Australian flora as a suitable source of isolates such as myrtenal (Astartea, myrtenol (Agonis, methyl chavicol (Ochrosperma, α-phellandren-8-ol (Prostanthera, methyl myrtenate (Darwinia, methyl geranate (Darwinia, kessane (Acacia, cis-dihydroagarofuran (Prosthanthera, protoanemonin (Clematis, isoamyl isovalerate (Micromyrtus, methyl cinnamate (Eucalyptus and bornyl acetate (Boronia. Many of these components are used, or have potential use in the fragrance, flavour, medicinal plant or insect attraction fields. Two weeds toxic to livestock in the Central West of the State are also harvested commercially as medicinal plants. Measurement of hypericin concentrations in the various plant parts of St John’s Wort (Hypericum perforatum over two seasons has shown that the weed can be effectively managed by grazing sheep during the winter months when toxin levels are low. Syntheses of β-carbolines tribulusterine and perlolyrine have shown that the former alkaloid was misidentified in the literature and hence not the toxic principle responsible for Tribulus staggers in sheep. Poor quality (high 1,8-cineole – low terpinen-4-ol oil bearing tea tree (Melaleuca alternifolia plantations have been established to the detriment of many a tea tree farmer. Analytical methods developed to check leaf quality at an early age indicated precursor sabinene constituents that convert to the


    Institute of Scientific and Technical Information of China (English)

    李高; 杜玉玲; 赵鸿彬


    为了探讨地被植物在不同遮荫条件下的适应性,本研究以10种地被植物为材料,对其在不同光照条件下的生长状态、日平均净光合速率、表观量子效率及光补偿点进行分析.结果表明:遮荫水平对10种植物的光合有很大影响,10种植物能忍受的遮荫程度不同.对旋覆花、甘野菊、大叶铁线莲、青杞、委陵菜来说,全光比较合适;对蛇莓、匍枝毛茛、匍枝委陵菜来说,它们在遮阴条件下的光合速率要高于在全光下的光合速率,1/3是最适遮荫度.而连钱草适宜的光强是1/3全光~全光,玉竹比较适宜1/6~1/3全光.根据试验材料对光的适应性,可将它们直接应用在不同的园林绿地中.%The study was focused on 10 ground covering plants in order to investigate the adaptabilities of the ground covering plants under different shade conditions. Their growth state, the average daily net photosynthetic rate, apparent quantum efficiency and light compensation point were analyzed under different illumination conditions. The results showed that shade levels have a great impact on photosynlhetic of the 10 plants. The tolerations of these plants were different under different levels of shade. Complete illumination were more suitable to Inula japonica, D. Lavandulifolium, Clematis heracleifolia, Solanum septemlobum Bge. And Potentilla chinensis Ser. However, the photosynthetic rate of Duchesnea indica, Potentilla eptans L. And Potentilla flagcllaris was higher than that in the full illumination, and 1/3 the illumination was the optimum shade degree to them. The appropriate light intensity to Glechoma hederacea L was 1/3 to full of the illumination, while Polygonatum doratum prefer 1/6 to 1/3 of the illumination. Those 10 ground covering plants can be applied directly to different gardens green space, according to the light adaptability of test materials.

  9. [Essentials of pharmacophylogeny: knowledge pedigree, epistemology and paradigm shift]. (United States)

    Hao, Da-cheng; Xiao, Pei-gen; Liu, Li-wei; Peng, Yong; He, Chun-nian


    Chinese materia medica resource (CMM resource) is the foundation of the development of traditional Chinese medicine. In the study of sustainable utilization of CMM resource, adopting innovative theory and method to find new CMM resource is one of hotspots and always highlighted. Pharmacophylogeny interrogates the phylogenetic relationship of medicinal organisms (especially medicinal plants), as well as the intrinsic correlation of morphological taxonomy, molecular phylogeny, chemical constituents, and therapeutic efficacy (ethnopharmacology and pharmacological activity). This new discipline may have the power to change the way we utilize medicinal plant resources and develop plant-based drugs. Phylogenomics is the crossing of evolutionary biology and genomics, in which genome data are utilized for evolutionary reconstructions. Phylogenomics can be integrated into the flow chart of drug discovery and development, and extends the field of pharmacophylogeny at the omic level, thus the concept of pharmacophylogenomics could be redefined in the context of plant pharmaceutical resources. This contribution gives a brief discourse of knowledge pedigree of pharmacophylogeny, epistemology and paradigm shift, highlighting the theoretical and practical values of pharmacophylogenomics. Many medicinally important tribes and genera, such as Clematis, Pulsatilla, Anemone, Cimicifugeae, Nigella, Delphinieae, Adonideae, Aquilegia, Thalictrum, and Coptis, belong to Ranunculaceae family. Compared to other plant families, Ranunculaceae has the most species that are recorded in China Pharmacopoeia (CP) 2010. However, many Ranunculaceae species, e. g., those that are closely related to CP species, as well as those endemic to China, have not been investigated in depth, and their phylogenetic relationship and potential in medicinal use remain elusive. As such, it is proposed to select Ranunculaceae to exemplify the utility of pharmacophylogenomics and to elaborate the new concept

  10. Preliminary studies on the evaluation system of endangered wild officinal plants in Mount Emei%峨眉山区野生濒危药用植物资源评价体系的初步研究

    Institute of Scientific and Technical Information of China (English)

    鲁松; 谢孔平; 李策宏


    The Emei Mountain area belongs to subtropical monsoon zone and is rich in officinal plants. Based on three years field investigation and literatures compilation, an evaluation system for endangered wild officinal plants in Mount Emei was established for the first time. The system contained seven indexes like storage and protection urgency. The results showed that 152 species,102 genera and 56 families were endangered species in the 1 655 wild officinal plants(including varieties and forms). 41 species,29 genera and 19 families which needed urgent protection accounted for 26. 97%, 28. 43% and 33. 93% respectively, and their representatives included Anoetochilus emeiensis, Coplis omeiensis ,Parakmeria omeiensis ,Bergenia emeinsis and so on. 35 species, 29 genera and 22 families which needed protection accounted for 23. 02% ,28. 43% ,39. 29% respectively,and their representatives included Manglie-tia szechuanica, Viburnum omeiensis , Lonicera similes and IIemsleya omeiensis. 76 species, 59 genera and 41 families which needed unclassified protection accounted for 50%, 57. 84% ,73. 21% respectively. And their representatives were Coplis chinensis ,Cinnamomum wilsonii ,Dysosma versipellis and Clematis chinensis.%峨眉山区属于亚热带季风气候区,野生药用植物资源十分丰富.经过三年的野外调查和文献资料整理,首次建立了峨眉山以蕴藏系数、保护缓急程度等7项指标在内的定量评价濒危药用植物指标体系.结果表明:峨眉山目前1 655种野生药用植物(含变种、变型)中,受到生存威胁的药用植物种类共56科102属152种.其中急需保护的有19科29属41种,分别占其科属种数的33.93%、28.43%和26.97%,代表种类主要有峨眉金线兰、峨眉黄连、峨眉拟单性木兰、峨眉岩白菜等;需要保护的有22科,29属,35种,分别占其科属种的39.29%、28.43%和23.02%,代表种类主要有四川木莲、峨眉荚蒾、峨眉忍冬、峨眉雪胆等;一般保护的有41科59

  11. Flower Species Identification and Coverage Estimation Based on Hyperspectral Remote Sensing Data in Hulunbeier Grassland%基于高光谱数据的呼伦贝尔草原花期物种识别和覆盖度估算

    Institute of Scientific and Technical Information of China (English)

    盖颖颖; 范闻捷; 徐希孺; 闫彬彦; 王焕炯; 刘媛


    实时准确地监测草地植物种类和覆盖面积对草原物种多样性研究和生态环境的可持续发展具有重要意义.草地植物在花期具有独特的光谱特征,相比营养生长期,通过花更容易识别物种.花期是遥感识别物种的关键时期.本文利用2008年8月和2010年7月呼伦贝尔草原上麻花头、棉闭铁线莲、冷蒿、莲子菜、黄花菜、有斑百合和细叶百合七种花以及裸土的实测高光谱数据,通过其冠层光谱的特征分析和参量化,找出了各物种之间的光谱差异,且得到了参量化特征识别方法,经验证,当花在样方中的覆盖度大于1o%时,识别方法的精度在90%以上.在此基础上,采用线性解混模型,计算样方中各种花的覆盖度,与实际数据相比较,误差在4%左右,证明了线性解混模型用于估算草原上花覆盖度的可行性.%Monitoring grassland species and area real-timely and accurately is of great significance in species diversity research, as well as in sustainable development of ecosystem. Flowers have their own unique spectral characteristics. Compared with the nutrient stage, species are more easily identified by florescence. So, florescence is a critical period for identification. In the present paper, spectral differences among such flowers as Galium verum Linn. , Hemerocallis citrina Baroni, Serratula centau-roides Linn. , Clematis hexapetala Pall. , Lilium concolor var. Pulchellum, Lilium pumilum and Artemisia frigida Willd. Sp. PL were found, along with identification methods, by analyzing canopies spectra and parametrizing characteristics. Verification results showed that when the coverage of flowers was greater than 10%, the accuracy of identification methods would be higher than 90%. On this basis, linear unmixing model was adopted to calculate the area of flowers in quadrates. Results showed that linear unmixing model was an effective method for estimating the coverage of flowers in grassland

  12. The terebinth population (Pistacia mutica Fisсh. & C.A. Mey. in the Besh-Tash valley (South-East Crimea

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    Viktoria Ju. Letukhova


    Full Text Available A comprehensive work on the study of rare and protected wood plants on the territory of the Besh-Tash valley (South-East Crimea was carried out on the instructions of Karadag Nature Reserve administration in 2013. The Besh-Tash valley (approximate area of 15 hectares is wedged in the territory of the Karadag reserve from the south-west side, but it is not included in its structure. This article describes the material on distribution, abundance, population structure of terebinth (Pistacia mutica in this area. P. mutica is a Mediterranean relict species of the Tertiary period, included in the Red Books of Russia, Crimea and Ukraine. Terebinth creates rare relict plant communities (formation P. mutica listed in the Green Book of Ukraine. In the study area we counted all P. mutica specimens. We determined their taxation parameters, as well as characterized their locality. Based on the literature and our own research age-state classes of P. mutica were characterized for the studied population. As a result, we registered a total number of 3086 specimens of P. mutica: immature (im – 1259 (40.8%, virginal (v – 1054 (34.2%, young generative (g1 – 341 (11.0%, middle-age generative (g2 – 372 (12.0%, mature generative (g3 – 60 (2.0% plants. Senile plants have not been found out. So the age spectrum of the population is normal, not complete (without senile individuals, left-sided with predominance of immature plants. The population density is 206 specimens/ha. In the Besh-Tash valley P. mutica forms its own dense thickets or it is the component of hemixerophytical oak forests with Quercus pubescens, Juniperus deltoides, rarer Cornus mas, Sorbus torminalis. It also grows in shrub communities with Pyrus elaeagrifolia, Paliurus spina-christi, Prunus spinosa, Rosa corymbifera, Cotoneaster tauricus, Crataegus species, rarer Cotinus coggygria, Clematis vitalba. The distribution of the terebinth tree by elevation above sea level is analyzed. The great

  13. Clinical Research on Huoxue Huayu Decoction and Manipulative Reduction in the Treatment of Thoracolumbar Compression Fracture%活血化瘀汤加手法复位治疗胸腰段压缩性骨折临床研究

    Institute of Scientific and Technical Information of China (English)



    Objective;To investigate the clinical efficacy of Huoxue Huayu Decoction and manipulative reduction in the treatment of tho-racolumbar compression fracture. Methods: 102 cases of thoraeolumbar compression fractures were randomly divided into and the treat-ment group of 52 cases and the control group of 50 cases. Both groups were advised to lie on the hardboard bed. The treatment group was given oral administration of Huoxue Huayu Decoction for the first 1-2 weeks (Recipe; walnuts, safflower, parsley, red peony, hematoxy-lin,12 g for each,frankincense,myrrh,aggregata,Dibiechong,citrus aurantium,plantain 6 g for each) ,Jiegu Xujin Decoction during 3 -5 weeks( Recipe-. Angelica, Salvia.Dipsaeus, white peony root,bidentata,habitat,natural copper,Cyperus each 12 g,peach kernel,saf-flower, psoralen,Drynaria each 10 g) ,and Jianshen Zhuangyao Decoction after 6 weeks(Recipe:Loranthaceae,heracleum hemsleyanum michaux,Codonopsis,Rehmannia,Eucommia,Woodwardia,medlar,Dipsacus each 12 g,Poria, Atractylodes,Clematis,Drynaria 10 g). One formula daily and 5 days as a course of treatment. Manipulative reduction was given within 1 week. The control group was given Sanqishang Tablet and manipulative reduction was given within 1 week. Results;X-ray was taken to recheck the replacement situation and the healed fracture after a course of treatment. A follow-up of the treatment group after 1 a was given and it showed that 35 cases re-covered(67. 3% ) ,17 cases were effectively treated(32.7% ) ,and the effective rate was 100.0% ;for the control group,20 cases recov-ered(40.0% ) ,25 cases were effectively treated(50. 0% ) , and five cases were not improved ( 10. 0% ) and the effective rate was 90. 0% . Conclusion;The clinical effic acy of Huoxue Huayu Decoction and manipulative reduction is significant in the treatment of tho-raeolumbar compression fracture.%目的:探讨活血化瘀汤加手法复位治疗胸腰段压缩性骨折的临床疗效.方法:将102例胸腰段压缩性骨

  14. 康复护理对肘尺管综合征临床综合治疗的价值分析

    Institute of Scientific and Technical Information of China (English)



      目的探讨康复护理在尺神经松解前移术的基础上应用通络疏经汤及理疗治疗肘尺管综各征的临床疗效.方法选取27例CuTS患者接奇偶数分成两组.治疗组14例,神经卡压平均病程为12年(6月~10年).对照组13例,神经卡压平均病程为10年(5月~9年).治疗组与对照组采取单纯尺神经松解前移手术治疗.治疗组在手术治疗的基础上加用通络疏经汤(主要药物:豨莶草、地龙、威灵仙、当归、牛膝).用药均为每日l剂,并用中频脉冲治疗仪进行理疗.结果治疗组取得显效率64.29%,总有效率92.86%.对照组显效率46.16%,总有效率76.92%;两组比较有显著性差异(P<0.05);两组在治疗后神经传导速度存在明显差异,治疗组在治疗前、后神经传导速度有统计学差异(△P<0.05).根据显示的结果,可以明确看出治疗组的治疗效果明显好于对照组.结论采用医护结合的方法治疗肘尺管综合征,可以有效的改善临床症状和提高肌电图(EMG)神经传导速度,缩短治疗时间,且有利于肢体功能的早日康复.%  objective to explore the rehabilitation nursing in the ulnar nerve release reach technique based on the application of removing drain the soup and physiotherapy treatment knee scale tube harness every sign of clinical curative effect. Methods select 27 cases of patients with CuTS after odd even into two groups. The treatment group in 14 cases, nerve viega profi-press average course for 12 years (June ~ 10 years). Control group (n = 13), nerve viega profi-press average course for 10 years (5 months ~ 9 years). The treatment group and control group take simple ulnar nerve release reach surgical treatment. The treatment group in surgical treatment on the basis of add a removing drain the soup (main drug:glandularstalk st.paulswortherb/common st.paulswort herb, earthworm, clematis root, angelica, achyranthes). Drugs are daily l agent, and

  15. 下肢静脉曲张中医证型和方药临床研究的数据挖掘%Data Mining of Syndromes and Formulae of Lower extremity Varicose Vein Based on Traditional Chinese Medicine Clinical Studies

    Institute of Scientific and Technical Information of China (English)

    司富春; 宋雪杰


    -nalysis. Excess pathogenesis occupied the majority in all syndrome pathogenesis,mainly being blood stasis, dampness evil,heat evil,qi stagnation,cold evil;deficiency pathogenesis were mainly qi deficiency and blood deficiency. Eighty-nine oral-taking prescriptions of lower extremity varicose vein were counted,48 were set prescriptions,the main types concluded blood stasis-removing prescription,heat-clearing and toxin-remo-ving prescription,qi-blood tonifying prescription,summer-heat clearing prescription,heat -clearing and damp-expelling prescription,channel-warming and cold-expelling prescription,the other 41 prescriptions were experimental prescription;27 external-using prescriptions of lower extremity varicose vein were coun-ted,3 were set prescriptions and 24 were experimental prescriptions. Twenty-seven oral-taking prescrip-tions of lower extremity varicose ulcer were counted,7 were set prescriptions and 20 were experimental pre-scriptions;34 external-using prescriptions of lower extremity varicose ulcer were counted,and they were all experimental prescriptions. Both oral-taking and external-using prescriptions of lower extremity varicose vein mainly were herbs of removing blood stasis,tonifying,clearing heat,expelling damp,relieving exterior e-vil,expelling wind-damp,such as Niuxi( Radix Achyranthes Bidentata),Honghua( Carthamus tinctorius), Chuanxiong( Ligusticum chuanxiong Hort ),Danggui( Angelica sinensis ),Huangqi( Astragalus membrana-ceus),Gancao( Radix liquiritiae ),Jinyinhua( Lonicera japonica ),Pugongying( Taraxacum mongolicum Hand),Chishao( Paeonia lactiflora Pall ),Fuling( Wolfiporiacocos ),Guizhi( Cinnamomum cassia Presl ), Shengma( Cimicifuga foetida ),Weilingxian( Clematis chinensis Osbeck ),Fangji( Aristolochia kaempferi Willd),and so on;Both oral-taking and external-using prescriptions of lower extremity varicose ulcer were mainly herbs of clearing heat,removing blood stasis,tonifying,such as Niuxi,Jinyinhua,Pugongying,Fuling, Gancao

  16. Редкие виды сосудистых растений на территории национального парка «Земля леопарда» (Приморский край, Россия

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    E. A. Pimenova


    етственно делению территории НП. В результате было собрано более 1500 образцов сосудистых растений, хранящихся в гербарной коллекции БСИ ДВО РАН (VBGI. В статье приводится аннотированный перечень 28 редких видов, выявленных на территории НП «Земля леопарда» и подтвержденных гербарными образцами. К редким отнесены 3 вида, включенные в “The IUCN Red List of Threatened Species”(2014, 14 видов из Красной книги РФ (2008, 20 видов из Красной книги Приморского края (2008 и 5 видов, редко встречающихся на исследуемой территории в силу различных природных или антропогенных факторов. Из наиболее редких видов в статье приведены: Panax ginseng C. A. Mey., Codonopsis pilosula Nannf., Meehania urticifolia Makino, Clematis koreana Kom.