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Sample records for clamped downhole seismic

  1. Development of a 400 Level 3C Clamped Downhole Seismic Receiver Array for 3D Borehole Seismic Imaging of Gas Reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Bjorn N. P. Paulsson

    2006-09-30

    Borehole seismology is the highest resolution geophysical imaging technique available today to the oil and gas industry for characterization and monitoring of oil and gas reservoirs. However, the industry's ability to perform high resolution 3D imaging of deep and complex gas reservoirs using borehole seismology has been hampered by the lack of acquisition technology necessary to record large volumes of high frequency, high signal-to-noise-ratio borehole seismic data. This project took aim at this shortcoming by developing a 400 level 3C clamped downhole seismic receiver array, and accompanying software, for borehole seismic 3D imaging. This large borehole seismic array has removed the technical acquisition barrier for recording the data volumes necessary to do high resolution 3D VSP and 3D cross-well seismic imaging. Massive 3D VSP{reg_sign} and long range Cross-Well Seismology (CWS) are two of the borehole seismic techniques that promise to take the gas industry to the next level in their quest for higher resolution images of deep and complex oil and gas reservoirs. Today only a fraction of the oil or gas in place is produced when reservoirs are considered depleted. This is primarily due to our lack of understanding of detailed compartmentalization of oil and gas reservoirs. In this project, we developed a 400 level 3C borehole seismic receiver array that allows for economic use of 3D borehole seismic imaging for reservoir characterization and monitoring. This new array has significantly increased the efficiency of recording large data volumes at sufficiently dense spatial sampling to resolve reservoir complexities. The receiver pods have been fabricated and tested to withstand high temperature (200 C/400 F) and high pressure (25,000 psi), so that they can operate in wells up to 7,620 meters (25,000 feet) deep. The receiver array is deployed on standard production or drill tubing. In combination with 3C surface seismic or 3C borehole seismic sources, the 400

  2. Development of a downhole seismic source with controlled waveform; Hakei seigyogata kochu shingen no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Kuroda, T.; Ikawa, T. [Japex Jeoscience Institute, Tokyo (Japan); Sato, T. [Meiho Engineering Co. Ltd., Tokyo (Japan); Kakuma, H. [Akashi Corp., Tokyo (Japan); Onuma, H. [Engineering Advancement Association of Japan, Tokyo (Japan)

    1997-05-27

    A downhole seismic source which can output continuous waves having arbitrary waveforms was developed. The development was targeted to make tomographic exploration purposed to evaluate geological properties of a ground bed before and after constructing a building in a ground several hundred meters deep from the ground surface. The source is considered to be used in an environment consisting of soft rocks or more robust rocks and having no casing. It can be used in a well hole having a diameter of 100 mm, is capable of measuring P and S waves in a distance between well holes of up to 100 m, can be used at a depth of up to 500 m, and can output waveforms having seismic source spectra of up to 1000 Hz. An oscillation actuator using laminated piezo-electric elements was used for the oscillation element. The seismic source consists of a hydraulic device to clamp the equipment onto hole walls, piezo-electric elements as the oscillation element, and an inertia weight for applying vibration from above and below. To make an oscillation, the main body is first clamped on the hole wall. For horizontal oscillation, the piezo-electric elements contained in a clamping device provide the horizontal oscillation. For vertical oscillation, the piezo-electric elements placed below the main body oscillates the inertia weight. The initially targeted specifications have been achieved. 3 refs., 4 figs., 1 tab.

  3. Seismic Observations in the Taipei Metropolitan Area Using the Downhole Network

    Directory of Open Access Journals (Sweden)

    Win-Gee Huang

    2010-01-01

    Full Text Available Underlain by soft soils, the Taipei Metropolitan Area (TMA experienced major damage due to ground-motion amplification during the Hualien earthquake of 1986, the Chi-Chi earthquake of 1999, the Hualien earthquake of 2002 and the Taitung earthquake of 2003. To study how a local site can substantially change the characteristics of seismic waves as they pass through soft deposits below the free surface, two complementary downhole seismic arrays have been operated in the TMA, since 1991 and 2008. The accelerometer downhole array is composed of eight boreholes at depths in excess of 300 meters. The downhole array velocity sensor collocated with accelerometer composed of four boreholes at depths up to 90 meters. The integrated seismic network monitors potential earthquakes originating from faults in and around the TMA and provides wide-dynamic range measurement of data ranging in amplitude from seismic background noise levels to damage levels as a result of shaking. The data sets can be used to address on the response of soft-soil deposits to ground motions. One of the major considerations is the nonlinear response of soft soil deposits at different levels of excitation. The collocated acceloerometer and velocity sensors at boreholes give the necessary data for studies of non-linearity to be acquired. Such measurements in anticipation of future large, damaging earthquakes will be of special importance for the mitigation of earthquake losses.

  4. Downhole seismic logging for high-resolution reflection surveying in unconsolidated overburden

    Energy Technology Data Exchange (ETDEWEB)

    Hunter, J.A.; Pullan, S.E.; Burns, R.A.; Good, R.L. [Geological Survey of Canada, Ottawa, Ontario (Canada); Harris, J.B. [Millsaps College, Jackson, MS (United States). Dept. of Geology; Pugin, A. [Univ. of Geneva (Switzerland). Dept. of Geology and Paleontology; Skvortsov, A. [Russian Academy of Sciences, Moscow (Russian Federation). Inst. of Earth Cryosphere; Goriainov, N.N. [Russian Committee of Geology, Noginsk (Russian Federation). Research Inst. for Hydrogeology and Engineering Geology

    1998-07-01

    Downhole seismic velocity logging techniques have been developed and applied in support of high-resolution reflection seismic surveys. Data obtained from downhole seismic logging can provide accurate velocity-depth functions and directly correlate seismic reflections to depth. The methodologies described in this paper are designed for slimhole applications in plastic-cased boreholes (minimum ID of 50 mm) and with source and detector arrays that yield similar frequency ranges and vertical depth resolutions as the surface reflection surveys. Compressional- (P-) wave logging uses a multichannel hydrophone array with 0.5-m detector spacings in a fluid-filled borehole and a high-frequency, in-hole shotgun source at the surface. Overlapping array positions downhole results in redundant first-arrival data which can be processed to provide accurate interval velocities. The data also can be displayed as a record suite, showing reflections and directly correlating reflection events with depths. Example applications include identification of gas zones, lithological boundaries within unconsolidated sediments, and the overburden-bedrock interface. Shear- (S-) wave logging uses a slimhole, well-locked, three-component (3-C) geophone pod and a horizontally polarized, hammer-and-loaded-plate source at ground surface. In unconsolidated sediments, shear-wave velocity contrasts can be associated with changes in material density or dynamic shear modulus, which in turn can be related to consolidation. Example applications include identification of a lithological boundary for earthquake hazard applications and mapping massive ice within permafrost materials.

  5. Near-surface attenuation using traffic-induced seismic noise at a downhole array

    Science.gov (United States)

    Dikmen, S. Umit; Pinar, Ali; Edincliler, Ayse

    2016-01-01

    A novel approach is developed for estimating the near-surface attenuation using seismic noise recordings at a downhole array. The amplitude spectrum of the traffic-induced seismic noise at the engineering bedrock level exhibits a high-frequency decay between 10 and 40 Hz. Subsequently, it yields a Kappa value of 14 ± 3 ms and a quality factor of 45 ± 10 for the profile between the highway and the sensor. Likewise, using the earthquake recordings made at the surface and the engineering bedrock levels, the Kappa values are calculated as 60 and 45 ms, respectively. The difference was attributed to near-surface attenuation where the upgoing earthquake waves and the downgoing traffic-induced seismic waves traverse similar soil profiles resulting in similar Kappa values. Hence, the near-site geology attenuation properties can be derived using the seismic noise data induced by a known source at a close distance recorded at engineering bedrock level.

  6. Comparison of lower-frequency (<1000 Hz) downhole seismic sources for use at environmental sites

    International Nuclear Information System (INIS)

    In conjunction with crosswell seismic surveying being done at the Hanford Site in south-central Washington, four different downhole seismic sources have been tested between the same set of boreholes. The four sources evaluated were the Bolt airgun, the OYO-Conoco orbital vibrator, and two Sandia-developed vertical vibrators, one pneumatically-driven, and the other based on a magnetostrictive actuator. The sources generate seismic energy in the lower frequency range of less than 1000 Hz and have different frequency characteristics, radiation patterns, energy levels, and operational considerations. Collection of identical data sets with all four sources allows the direct comparison of these characteristics and an evaluation of the suitability of each source for a given site and target

  7. DOWNHOLE SEISMIC REFRACTION SURVEY OF WEATHERED LAYER CHARACTERISTICS IN ESCRAVOS, NIGERIA

    Directory of Open Access Journals (Sweden)

    Ochuko Anomohanran

    2014-01-01

    Full Text Available The problem of datum correction in seismic surveys and the failures associated with civil engineering structures with specific reference to roads and buildings has been attributed to lack of understanding of the soil structure among others. This is why this survey on downhole seismic refraction was conducted from nine borehole locations in Escravos, Nigeria to determine the weathered layer characteristics of the area. In carrying out this study, the McSeis 160MX instrument was employed in recording data obtained from a set of hydrophones arranged in a definite manner in each of the boreholes. The data were analysed graphically by using the seismic refraction interpretation techniques. Results of interpretation indicate that the velocity of the weathered layer ranged between 454 and 533 m sec-1 with an average value of 495 m sec-1. The results also revealed that the depth of the weathered layer ranged between 2.91 and 4.41 m with an average value of 3.68 m. Analysis of the results revealed that the depth and velocity of the weathered layer increased downward towards the southern part of the study area. This is an indication that the weathered layer sloped downward to the south. It is recommended that shots for seismic survey should be located at a depth of 4.41 m to eliminate the effects associated with the low velocity layer. It is also recommended that construction of heavy buildings and roads especially bridges should have their foundations laid to a depth of 4.41 m to enable the infrastructure sit on the consolidated layer.

  8. Application of multi-stage, multi-disk type downhole seismic source; Tadanshiki taso enbangata koseinai shingen no tekiyosei

    Energy Technology Data Exchange (ETDEWEB)

    Shimada, N. [Japan National Oil Corp., Tokyo (Japan); Shoji, Y. [Oyo Corp., Tokyo (Japan)

    1997-05-27

    A multi-stage, multi-disk type seismic source was developed as a downhole seismic source. The seismic source is an improved version of the downhole seismic source of a system in which an elastic wave is generated by a weight accelerated by restitutive force of a spring striking the upper part of a laminated structure consisted of metal disks and elastic bodies installed in water in a well. Enhancing the vibration exciting efficiency requires impedance radiated from the disks to be increased. The multi-disk structure was adopted because of restrictions on the disk area under the limiting condition of being inside the well. Further limitation has still existed, which led to finally structuring the multi-disk type to a multi-stage construction to increase the radiated impedance. In order to increase average velocity on the radiation surface, mass relationship between the hammer and the anvil was sought so that the maximum velocity is achieved at the process of converting motion energies among the hammer, anvil and disks. The anvil mass may sufficiently be 50% to 100% of the hammer mass. The equipment was installed in an actual oil well for testing. This seismic source was verified to have sufficient applicability in the cross hole measurement. 5 refs., 7 figs., 1 tab.

  9. Observations Using the Taipei Basin Broadband Downhole Seismic Network: The 26 December 2006, Pingtung Earthquake Doublet, Taiwan

    Directory of Open Access Journals (Sweden)

    Win-Gee Huang

    2008-01-01

    Full Text Available To monitor the fault activity in the Taipei area, a new broadband downhole seismic network comprised of three stations was established in the Taipei Basin over a period of three years, 2005 - 2007. The network geometry is a triangle with a station spacing of about 12 km covering the entire Taipei Basin. Each station has two holes of different depths containing modern instruments, including a low-gain broadband seismometer. The largest depth is 150 m. We report our first experience on the installation and operation of the broadband downhole seismic network in the Taipei Basin. Some representative records from the Pingtung earthquake doublet in December 2006 are shown here. Ground displacement during the Pingtung earthquake doublet can be recovered from the velocity records without the baseline corrections that are required for the acceleration records. Our network offers excellent data for accurate and effective characterization of seismic motion in the study area. Seismic data from this network will be shared with other research institutions in Taiwan and abroad for further research.

  10. Observations Using the Taipei Basin Broadband Downhole Seismic Network: The 26 December 2006, Pingtung Earthquake Doublet, Taiwan

    OpenAIRE

    Win-Gee Huang; Bor-Shouh Huang; Kou-Cheng Chen; Chun-Chi Liu; Chin-Ren Lin; Shu-Hjong Tsao; Yu-Chung Hsieh; and Chi-Hsuan Chen

    2008-01-01

    To monitor the fault activity in the Taipei area, a new broadband downhole seismic network comprised of three stations was established in the Taipei Basin over a period of three years, 2005 - 2007. The network geometry is a triangle with a station spacing of about 12 km covering the entire Taipei Basin. Each station has two holes of different depths containing modern instruments, including a low-gain broadband seismometer. The largest depth is 150 m. We report our first experience on the inst...

  11. Applicability of fiber model in seismic response analysis for center-clamp type bushings on transformers

    International Nuclear Information System (INIS)

    A bushing is a device for insulation and support of a conductor. Especially it is called center-clamp type when it is connected with a metal holder through clamping force. As a consequence of damage of center-clamp type bushings in Tohoku Earthquake in 2011, refinement of the response analysis method and review of the seismic design procedure became necessary. In the present report, the fiber model, which can evaluate non-linear behavior in the section subjected to axial force and bending moment, was implemented to the CRIEPI's finite element analysis program 'Mastrd' considering following characteristics of center-clamp type bushings. a) The gasket section between a porcelain tube and a metal holder has a torus shape. b) Springs around the top of the bushing give clamping force, but they lock in excessive base rotation. c) The gasket does not resist against tension. d) Local resistance against compression due to bending increases in use of very thin gaskets. The developed program was verified through comparison with the shaking table test result for real bushings whose voltage classes were 154 kV and 275 kV. Deformation indices as rotation angle and base opening due to bending were influenced by damping conditions. Though there was not the condition which brought about remarkable underestimation, reduction of damping for a fiber model element was preferable for safety. On the other hand, bending moment was consistent with experimental results because it tended not to fluctuate in the non-linear region. (author)

  12. Down-hole seismic survey system with fiber-optic accelerometer sensor array for 3-dimensions vertical seismic profile (3D-VSP)

    Science.gov (United States)

    Zou, Qilin; Wang, Liwei; Pang, Meng; Tu, Dongsheng; Zhang, Min; Liao, Yanbiao

    2006-08-01

    We demonstrated a down-hole seismic survey system that can be applied in three dimensions vertical seismic profile (VSP) detection in petroleum exploration. The results of experiments show that the system has a dynamic measurement range of 80db (ratio of maximum to minimum value) and the total delay for signal collection, process and communication is less than 200ms @ 2k bit sample rates. An array consisting of six fiber-optic accelerometers (receivers) is applied in this system. Each receiver is comprised of three fiber-optic Michelson interferometers. In order to meet the requirements of high precision and real-time measurement, the high-speed DSP chips are employed to realize the algorithms of signal filters and Phase Generated Carrier (PGC) demodulation to obtain the seismic information. Multi-ARM CPUs are introduced into the system to design the fiber-optic accelerometer array controller and the receiver array local bus that are used for real-time data communication between the multi-level receivers and controller. The system interface for traditional ELIS Down-hole Instrument Bus (EDIB) is designed by the use of FPGA so that our system can attach to EDIB and cooperate with other instruments. The design and experiments of the system are given in this paper in detail.

  13. Downhole tool adapted for telemetry

    Science.gov (United States)

    Hall, David R.; Fox, Joe

    2010-12-14

    A cycleable downhole tool such as a Jar, a hydraulic hammer, and a shock absorber adapted for telemetry. This invention applies to other tools where the active components of the tool are displaced when the tool is rotationally or translationally cycled. The invention consists of inductive or contact transmission rings that are connected by an extensible conductor. The extensible conductor permits the transmission of the signal before, after, and during the cycling of the tool. The signal may be continuous or intermittent during cycling. The invention also applies to downhole tools that do not cycle, but in operation are under such stress that an extensible conductor is beneficial. The extensible conductor may also consist of an extensible portion and a fixed portion. The extensible conductor also features clamps that maintain the conductor under stresses greater than that seen by the tool, and seals that are capable of protecting against downhole pressure and contamination.

  14. Geological controls on the occurrence of gas hydrate from core, downhole log, and seismic data in the Shenhu area, South China Sea

    Science.gov (United States)

    Xiujuan Wang; Xiujuan Wang; Collett, Timothy S.; Lee, Myung W.; Yang, Shengxiong; Guo, Yiqun; Wu, Shiguo

    2014-01-01

    Multi-channel seismic reflection data, well logs, and recovered sediment cores have been used in this study to characterize the geologic controls on the occurrence of gas hydrate in the Shenhu area of the South China Sea. The concept of the "gas hydrate petroleum system" has allowed for the systematic analysis of the impact of gas source, geologic controls on gas migration, and the role of the host sediment in the formation and stability of gas hydrates as encountered during the 2007 Guangzhou Marine Geological Survey Gas Hydrate Expedition (GMGS-1) in the Shenhu area. Analysis of seismic and bathymetric data identified seventeen sub-linear, near-parallel submarine canyons in this area. These canyons, formed in the Miocene, migrated in a northeasterly direction, and resulted in the burial and abandonment of canyons partially filled by coarse-grained sediments. Downhole wireline log (DWL) data were acquired from eight drill sites and sediment coring was conducted at five of these sites, which revealed the presence of suitable reservoirs for the occurrence of concentrated gas hydrate accumulations. Gas hydrate-bearing sediment layers were identified from well log and core data at three sites mainly within silt and silt clay sediments. Gas hydrate was also discovered in a sand reservoir at one site as inferred from the analysis of the DWL data. Seismic anomalies attributed to the presence of gas below the base of gas hydrate stability zone, provided direct evidence for the migration of gas into the overlying gas hydrate-bearing sedimentary sections. Geochemical analyses of gas samples collected from cores confirmed that the occurrence of gas hydrate in the Shenhu area is controlled by the presence thermogenic methane gas that has migrated into the gas hydrate stability zone from a more deeply buried source.

  15. Insulated pipe clamp design

    International Nuclear Information System (INIS)

    Thin wall large diameter piping for breeder reactor plants can be subjected to significant thermal shocks during reactor scrams and other upset events. On the Fast Flux Test Facility, the addition of thick clamps directly on the piping was undesired because the differential metal temperatures between the pipe wall and the clamp could have significantly reduced the pipe thermal fatigue life cycle capabilities. Accordingly, an insulated pipe clamp design concept was developed. The design considerations and methods along with the development tests are presented. Special considerations to guard against adverse cracking of the insulation material, to maintain the clamp-pipe stiffness desired during a seismic event, to minimize clamp restraint on the pipe during normal pipe heatup, and to resist clamp rotation or spinning on the pipe are emphasized

  16. Multichannel analysis of surface-waves and integration of downhole acoustic televiewer imaging, ultrasonic Vs and Vp, and vertical seismic profiling in an NEHRP-standard classification, South of Concordia, Kansas, USA

    Science.gov (United States)

    Raef, Abdelmoneam; Gad, Sabreen; Tucker-Kulesza, Stacey

    2015-10-01

    Seismic site characteristics, as pertaining to earthquake hazard reduction, are a function of the subsurface elastic moduli and the geologic structures. This study explores how multiscale (surface, downhole, and laboratory) datasets can be utilized to improve "constrained" average Vs30 (shear-wave velocity to a 30-meter depth). We integrate borehole, surface and laboratory measurements for a seismic site classification based on the standards of the National Earthquake Hazard Reduction Program (NEHRP). The seismic shear-wave velocity (Vs30) was derived from a geophysical inversion workflow that utilized multichannel analysis of surface-waves (MASW) and downhole acoustic televiewer imaging (DATI). P-wave and S-wave velocities, based on laboratory measurements of arrival times of ultrasonic-frequency signals, supported the workflow by enabling us to calculate Poisson's ratio, which was incorporated in building an initial model for the geophysical inversion of MASW. Extraction of core samples from two boreholes provided lithology and thickness calibration of the amplitudes of the acoustic televiewer imaging for each layer. The MASW inversion, for calculating Vs sections, was constrained with both ultrasonic laboratory measurements (from first arrivals of Vs and Vp waveforms at simulated in situ overburden stress conditions) and the downhole acoustic televiewer (DATV) amplitude logs. The Vs30 calculations enabled categorizing the studied site as NEHRP-class "C" - very dense soil and soft rock. Unlike shallow fractured carbonates in the studied area, S-wave and P-wave velocities at ultrasonic frequency for the deeper intact shale core-samples from two boreholes were in better agreement with the corresponding velocities from both a zero-offset vertical seismic profiling (VSP) and inversion of Rayleigh-wave velocity dispersion curves.

  17. Gas hydrate identified in sand-rich inferred sedimentary section using downhole logging and seismic data in Shenhu area, South China Sea

    Science.gov (United States)

    Wang, Xiujuan; Lee, Myung W.; Collett, Timothy S.; Yang, Shengxiong; Guo, Yiqun; Wu, Shiguo

    2014-01-01

    Downhole wireline log (DWL) data was acquired from eight drill sites during China's first gas hydrate drilling expedition (GMGS-1) in 2007. Initial analyses of the acquired well log data suggested that there were no significant gas hydrate occurrences at Site SH4. However, the re-examination of the DWL data from Site SH4 indicated that there are two intervals of high resistivity, which could be indicative of gas hydrate. One interval of high resistivity at depth of 171–175 m below seafloor (mbsf) is associated with a high compressional- wave (P-wave) velocities and low gamma ray log values, which suggests the presence of gas hydrate in a potentially sand-rich (low clay content) sedimentary section. The second high resistivity interval at depth of 175–180 mbsf is associated with low P-wave velocities and low gamma values, which suggests the presence of free gas in a potentially sand-rich (low clay content) sedimentary section. Because the occurrence of free gas is much shallower than the expected from the regional depth of the bottom simulating reflector (BSR), the free gas could be from the dissociation of gas hydrate during drilling or there may be a local anomaly in the depth to the base of the gas hydrate stability zone. In order to determine whether the low P-wave velocity with high resistivity is caused by in-situ free gas or dissociated free gas from the gas hydrate, the surface seismic data were also used in this analysis. The log analysis incorporating the surface seismic data through the construction of synthetic seismograms using various models indicated the presence of free gas directly in contact with an overlying gas hydrate-bearing section. The occurrence of the anomalous base of gas hydrate stability at Site SH4 could be caused by a local heat flow conditions. This paper documents the first observation of gas hydrate in what is believed to be a sand-rich sediment in Shenhu area of the South China Sea.

  18. Three phase downhole separator process

    Science.gov (United States)

    Cognata, Louis John

    2008-06-24

    Three Phase Downhole Separator Process (TPDSP) is a process which results in the separation of all three phases, (1) oil, (2) gas, and (3) water, at the downhole location in the well bore, water disposal injection downhole, and oil and gas production uphole.

  19. Temperature compensating stiff pipe clamp

    International Nuclear Information System (INIS)

    A new type of non-integral pipe attachment for nuclear piping seismic restraint that allows the pipe free thermal diametric expansion without constraint when using dissimilar pipe and clamp material is described. The clamp has a high spring rate that can be controlled by variable stiffness parameters in the design. Described in detail are thermal constraint stress, load stress distribution, spring rates, load angles and design philosophy. Analytical methods of code design, fabrication techniques, cost benefits and lead time reduction techniques are presented. 5 refs

  20. Distributed downhole drilling network

    Science.gov (United States)

    Hall, David R.; Hall, Jr., H. Tracy; Fox, Joe; Pixton, David S.

    2006-11-21

    A high-speed downhole network providing real-time data from downhole components of a drilling strings includes a bottom-hole node interfacing to a bottom-hole assembly located proximate the bottom end of a drill string. A top-hole node is connected proximate the top end of the drill string. One or several intermediate nodes are located along the drill string between the bottom-hole node and the top-hole node. The intermediate nodes are configured to receive and transmit data packets transmitted between the bottom-hole node and the top-hole node. A communications link, integrated into the drill string, is used to operably connect the bottom-hole node, the intermediate nodes, and the top-hole node. In selected embodiments, a personal or other computer may be connected to the top-hole node, to analyze data received from the intermediate and bottom-hole nodes.

  1. Downhole burner for wells

    Energy Technology Data Exchange (ETDEWEB)

    Brandt, H.; Hazard, H.R.; Hummell, J.D.; Schulz, E.J.

    1966-03-22

    This is a downhole gas and air burner for use in wells to stimulate production. The combustible mixture is supplied to the combustion chamber of the downhole burner through a delivery tube. This tube includes a flow-back preventer and a check valve. The flashback preventers consist of a porous material which has restricted flow paths. The check valve controls the flow of combustible mixture to the combustion chamber and prevents undesirable pulsating flow through the combustion chamber and the delivery tube. The check valve also prevents flooding of the combustion chamber by well fluid. The burner is ignited electrically. The porous material can be flat strip or a conically shaped piece of thin porous metal.

  2. Downhole pressure attenuation apparatus

    International Nuclear Information System (INIS)

    This patent describes a process for preventing damage to tool strings and other downhole equipment in a well caused by pressures produced during detonation of one or more downhole explosive devices. It comprises adding to a tool string at least one pressure attenuating apparatus for attenuating the peak pressure wave and quasi-static pressure pulse produced by the explosive devices, the pressure attenuating apparatus including an initially closed relief vent including tubing means supporting a plurality of charge port assemblies each including an explosive filled shaped charge and a prestressed disc, the shaped charges interconnected by a detonating cord, the amount of explosive in each shaped charge being sufficient to rupture its associated disc without damaging surrounding tubular bodies in the well, and a vent chamber defined by the tubing means and providing a liquid free volume, and opening the relief vent substantially contemporaneously with downhole explosive device detonation by detonating the shaped charges to rupture the discs of the charge port assemblies

  3. Downhole Fluid Analyzer Development

    Energy Technology Data Exchange (ETDEWEB)

    Bill Turner

    2006-11-28

    A novel fiber optic downhole fluid analyzer has been developed for operation in production wells. This device will allow real-time determination of the oil, gas and water fractions of fluids from different zones in a multizone or multilateral completion environment. The device uses near infrared spectroscopy and induced fluorescence measurement to unambiguously determine the oil, water and gas concentrations at all but the highest water cuts. The only downhole components of the system are the fiber optic cable and windows. All of the active components--light sources, sensors, detection electronics and software--will be located at the surface, and will be able to operate multiple downhole probes. Laboratory testing has demonstrated that the sensor can accurately determine oil, water and gas fractions with a less than 5 percent standard error. Once installed in an intelligent completion, this sensor will give the operating company timely information about the fluids arising from various zones or multilaterals in a complex completion pattern, allowing informed decisions to be made on controlling production. The research and development tasks are discussed along with a market analysis.

  4. To clamp or not to clamp late?

    Directory of Open Access Journals (Sweden)

    Nicoletta Iacovidou

    2013-04-01

    Full Text Available When resuscitating a neonate, one encounters controversial issues and a great deal of confusion. These issues include the question as to when should the umbilical cord be clamped. Clamping and cutting the umbilical cord at birth is the oldest intervention in humans. Even though many aspects of the timing for cord clamping have been clarified, some questions still remain unanswered. If delayed cord clamping is to become a standard approach which timing is the optimal? And depending on the mode of delivery should timing be the same or different for normal vaginal delivery or for caesarean section? What about  the compromised neonate, or the very preterm one? We have more questions than answers. More randomized controlled studies are needed in normal and pathologic neonates, in order to address these questions, before we practice delayed cord clamping with safety for the babies and the mothers.

  5. Servodrive Clamping Unit

    OpenAIRE

    Štefánek, Tomáš

    2009-01-01

    Design servodrive clamping unit with pneumatic or mechanic multiplier. Unit has be instrumental to strength clamp parts in tension technical machines. Frame unit’s is complicate from massive and movable clamping board and is self-supporting. Unit has be solution like incorporated modulus. Target is proposal main driving period for movable clamping board. Work up proposal constructional solution, drawing listings, production drawing of the three engaged part of, BOM. Computational report must ...

  6. Photovoltaic panel clamp

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Malcolm P.; Mittan, Margaret Birmingham; Miros, Robert H. J.; Stancel, Robert

    2013-03-19

    A photovoltaic panel clamp includes an upper and lower section. The interface between the assembled clamp halves and the module edge is filled by a flexible gasket material, such as EPDM rubber. The gasket preferably has small, finger like protrusions that allow for easy insertion onto the module edge while being reversed makes it more difficult to remove them from the module once installed. The clamp includes mounting posts or an integral axle to engage a bracket. The clamp also may include a locking tongue to secure the clamp to a bracket.

  7. Battery switch for downhole tools

    Science.gov (United States)

    Boling, Brian E.

    2010-02-23

    An electrical circuit for a downhole tool may include a battery, a load electrically connected to the battery, and at least one switch electrically connected in series with the battery and to the load. The at least one switch may be configured to close when a tool temperature exceeds a selected temperature.

  8. Laser beam guard clamps

    Science.gov (United States)

    Dickson, Richard K.

    2010-09-07

    A quick insert and release laser beam guard panel clamping apparatus having a base plate mountable on an optical table, a first jaw affixed to the base plate, and a spring-loaded second jaw slidably carried by the base plate to exert a clamping force. The first and second jaws each having a face acutely angled relative to the other face to form a V-shaped, open channel mouth, which enables wedge-action jaw separation by and subsequent clamping of a laser beam guard panel inserted through the open channel mouth. Preferably, the clamping apparatus also includes a support structure having an open slot aperture which is positioned over and parallel with the open channel mouth.

  9. Corrosion reference for geothermal downhole materials selection

    Energy Technology Data Exchange (ETDEWEB)

    Ellis, P.F. II, Smith, C.C.; Keeney, R.C.; Kirk, D.K.; Conover, M.F.

    1983-03-01

    Geothermal downhole conditions that may affect the performance and reliability of selected materials and components used in the drilling, completion, logging, and production of geothermal wells are reviewed. The results of specific research and development efforts aimed at improvement of materials and components for downhole contact with the hostile physicochemical conditions of the geothermal reservoir are discussed. Materials and components covered are tubular goods, stainless steels and non-ferrous metals for high-temperature downhole service, cements for high-temperature geothermal wells, high-temperature elastomers, drilling and completion tools, logging tools, and downhole pumps. (MHR)

  10. Clamping characteristics study on different types of clamping unit

    International Nuclear Information System (INIS)

    Plastic products are becoming more and more widely used in aerospace, IT, digital electronics and many other fields. With the development of technology, the requirement of product precision is getting higher and higher. However, type and working performance of clamping unit play a decisive role in product precision. Clamping characteristics of different types of clamping unit are discussed in this article, which use finite element numerical analysis method through the software ABAQUS to study the clamping uniformity, and detect the clamping force repeatability precision. The result shows that compared with toggled three-platen clamping unit, clamping characteristics of internal circulation two-platen clamping unit are better, for instance, its mold cavity deformation and force that bars and mold parting surface suffered are more uniform, and its clamping uniformity and repeatability precision is also better

  11. Neuronal Response Clamp

    Directory of Open Access Journals (Sweden)

    Avner Wallach

    2011-04-01

    Full Text Available Responses of individual neurons to ongoing input are highly variable, reflecting complex threshold dynamics. Experimental access to this threshold dynamics is required in order to fully characterize neuronal input-output relationships. The challenge is practically intractable using present day experimental paradigms due to the cumulative, nonlinear interactions involved. Here we introduce the Neuronal Response Clamp, a closed-loop technique enabling control over the instantaneous response probability of the neuron. The potential of the technique is demonstrated by showing direct access to threshold dynamics of cortical neuron in-vitro using extracellular recording and stimulation, over timescales ranging from seconds to many hours. Moreover, the method allowed us to expose the sensitivity of threshold dynamics to spontaneous input from the network in which the neuron is embedded. The Response Clamp technique follows the rationale of the voltage-clamp and dynamic-clamp approaches, extending it to the neuron's spiking behavior. The general framework offered here is applicable in the study of other neural systems, beyond the single neuron level.

  12. Cantilever clamp fitting

    Science.gov (United States)

    Melton, Patrick B. (Inventor)

    1989-01-01

    A device is disclosed for sealing and clamping a cylindrical element which is to be attached to an object such as a wall, a pressurized vessel or another cylindrical element. The device includes a gland having an inner cylindrical wall, which is threaded at one end and is attached at a bendable end to a deformable portion, which in turn is attached to one end of a conical cantilever structure. The other end of the cantilever structure connects at a bendable area to one end of an outer cylindrical wall. The opposite end of cylindrical wall terminates in a thickened portion, the radially outer surface of which is adapted to accommodate a tool for rotating the gland. The terminal end of cylindrical wall also includes an abutment surface, which is adapted to engage a seal, which in turn engages a surface of a receiver. The receiver further includes a threaded portion for engagement with the threaded portion of gland whereby a tightening rotation of gland relative to receiver will cause relative movement between cylindrical walls and of gland. This movement causes a rotation of the conical structure and thus a bending action at bending area and at the bending end of the upper end of inner cylindrical wall. These rotational and bending actions result in a forcing of the deformable portion radially inwardly so as to contact and deform a pipe. This forcible contact creates a seal between gland and pipe, and simultaneously clamps the pipe in position.

  13. 275 C Downhole Microcomputer System

    Energy Technology Data Exchange (ETDEWEB)

    Chris Hutchens; Hooi Miin Soo

    2008-08-31

    An HC11 controller IC and along with serial SRAM and ROM support ICs chip set were developed to support a data acquisition and control for extreme temperature/harsh environment conditions greater than 275 C. The 68HC11 microprocessor is widely used in well logging tools for control, data acquisition, and signal processing applications and was the logical choice for a downhole controller. This extreme temperature version of the 68HC11 enables new high temperature designs and additionally allows 68HC11-based well logging tools and MWD tools to be upgraded for high temperature operation in deep gas reservoirs, The microcomputer chip consists of the microprocessor ALU, a small boot ROM, 4 kbyte data RAM, counter/timer unit, serial peripheral interface (SPI), asynchronous serial interface (SCI), and the A, B, C, and D parallel ports. The chip is code compatible with the single chip mode commercial 68HC11 except for the absence of the analog to digital converter system. To avoid mask programmed internal ROM, a boot program is used to load the microcomputer program from an external mask SPI ROM. A SPI RAM IC completes the chip set and allows data RAM to be added in 4 kbyte increments. The HC11 controller IC chip set is implemented in the Peregrine Semiconductor 0.5 micron Silicon-on-Sapphire (SOS) process using a custom high temperature cell library developed at Oklahoma State University. Yield data is presented for all, the HC11, SPI-RAM and ROM. The lessons learned in this project were extended to the successful development of two high temperature versions of the LEON3 and a companion 8 Kbyte SRAM, a 200 C version for the Navy and a 275 C version for the gas industry.

  14. 2750 C Downhole Microcomputer System

    International Nuclear Information System (INIS)

    An HC11 controller IC and along with serial SRAM and ROM support ICs chip set were developed to support a data acquisition and control for extreme temperature/harsh environment conditions greater than 275 C. The 68HC11 microprocessor is widely used in well logging tools for control, data acquisition, and signal processing applications and was the logical choice for a downhole controller. This extreme temperature version of the 68HC11 enables new high temperature designs and additionally allows 68HC11-based well logging tools and MWD tools to be upgraded for high temperature operation in deep gas reservoirs, The microcomputer chip consists of the microprocessor ALU, a small boot ROM, 4 kbyte data RAM, counter/timer unit, serial peripheral interface (SPI), asynchronous serial interface (SCI), and the A, B, C, and D parallel ports. The chip is code compatible with the single chip mode commercial 68HC11 except for the absence of the analog to digital converter system. To avoid mask programmed internal ROM, a boot program is used to load the microcomputer program from an external mask SPI ROM. A SPI RAM IC completes the chip set and allows data RAM to be added in 4 kbyte increments. The HC11 controller IC chip set is implemented in the Peregrine Semiconductor 0.5 micron Silicon-on-Sapphire (SOS) process using a custom high temperature cell library developed at Oklahoma State University. Yield data is presented for all, the HC11, SPI-RAM and ROM. The lessons learned in this project were extended to the successful development of two high temperature versions of the LEON3 and a companion 8 Kbyte SRAM, a 200 C version for the Navy and a 275 C version for the gas industry

  15. A microelectromechanical system digital 3C array seismic cone penetrometer

    OpenAIRE

    Ghose, R.

    2012-01-01

    A digital 3C array seismic cone penetrometer has been developed for multidisciplinary geophysical and geotechnical applications. Seven digital triaxial microelectromechanical system accelerometers are installed at 0.25-m intervals to make a 1.5-m-long downhole seismic array. The accelerometers have a flat response up to 2 kHz. The seismic array is attached to a class 1 digital seismic cone, which measures cone tip resistance, sleeve friction, pore-pressure, and inclination. The downhole 3C ar...

  16. Downhole Elemental Analysis with LIBS

    Science.gov (United States)

    Moreschini, Paolo; Zacny, Kris; Rickman, Doug

    2011-01-01

    vertical stage; a second actuator at the top of the downhole probe allows radial scanning of the borehole. Analysis of iron and titanium in lunar simulant with LIBS was performed in air using the method of standard addition. The results for lunar simulant NU-LHT-2M show a value for the concentration of iron ranging between 2.29% and 3.05% depending on the atomic line selected. The accepted value for the sample analyzed is 2.83%, showing the capability for the system in development to provide qualitative and semi-quantitative analysis in real-time.

  17. Downhole elemental analysis with LIBS

    Science.gov (United States)

    Moreschini, P.; Zacny, K.; Rickman, D.

    2011-12-01

    top of the downhole probe allows radial scanning of the borehole. Analysis of iron and titanium in lunar simulant with LIBS was performed in air using the method of standard addition. The results for lunar simulant NU-LHT-2M show a value for the concentration of iron ranging between 2.29% and 3.05% depending on the atomic line selected. The accepted value for the sample analyzed is 2.83%, showing the capability for the system in development to provide qualitative and semi-quantitative analysis in real-time.

  18. PKM Mechatronic Clamping Adaptive Device

    OpenAIRE

    Alberto Borboni; Francesco Aggogeri; Angelo Merlo; Nicola Pellegrini; Cinzia Amici

    2015-01-01

    This study proposes a novel adaptive fixturing device based on active clamping systems for smart micropositioning of thin-walled precision parts. The modular architecture and the structure flexibility make the system suitable for various industrial applications. The proposed device is realized as a Parallel Kinematic Machine (PKM), opportunely sensorized and controlled, able to perform automatic error-free workpiece clamping procedures, drastically reducing the overall fixturing set-up time. ...

  19. Seismic Velocity Study of the Rim Uplift of the Steen River Impact Crater

    Science.gov (United States)

    Niccoli, M.; Hildebrand, A. R.; Lawton, D. C.

    2005-03-01

    The structure of the rim uplift at the 25 km diameter Steen River has been explored by 2D and 3D seismic reflection and refraction techniques, constrained by downhole well surveys. A complicated deformation history is revealed.

  20. Downhole drilling network using burst modulation techniques

    Science.gov (United States)

    Hall; David R. , Fox; Joe

    2007-04-03

    A downhole drilling system is disclosed in one aspect of the present invention as including a drill string and a transmission line integrated into the drill string. Multiple network nodes are installed at selected intervals along the drill string and are adapted to communicate with one another through the transmission line. In order to efficiently allocate the available bandwidth, the network nodes are configured to use any of numerous burst modulation techniques to transmit data.

  1. Downhole dehydration - status report and implementation study; Downhole Dehydration - Statusbericht und Umsetzungsstudie

    Energy Technology Data Exchange (ETDEWEB)

    Jacobs, D.; Schmidt, D. [BEB Erdgas und Erdoel GmbH, Hannover (Germany)

    1998-12-31

    Downhole dehydration, i.e. in-situ separation of water and oil, is an interesting new technology. The contribution describes the technology and the results of a field experiment. (orig.) [Deutsch] Die Tail-End Foerderphase in der deutschen Erdoelproduktion, welche durch hohe Wasserhebekosten gekennzeichnet ist, erfordert zur Aufrechterhaltung der Wirtschaftlichkeit neue Gedankenansaetze. Ein aus wirtschaftlicher und technischer Sicht reizvoller Optimierungsgedanke ist die untertaegige Wasser/Oel Separation, auch Downhole Dehydration genannt. Unter Downhole Dehydration (DHD) versteht man also die untertaegige (teilweise) Separation des Lagerstaettenwassers vom Nassoel, kurz nachdem das Gemisch untertaegig in das Bohrloch eingetreten ist. Dabei wird das abgetrennte Lagerstaettenwasser untertage in einen geeigneten Horizont unmittelbar wieder injiziert und das Oel (wie bei der konventionellen Foerderung) zutage gepumpt, mit dem Ziel der Nutzung der daraus resultierenden Kosten- und Investitionsersparnis. Ziel dieses Vortrages ist es, einen kurzen Einblick in die erstmalig in Europa angewandte Technik zu geben und von den Erfahrungen des praktizierten Feldversuches zu berichten. (orig.)

  2. Clamp wins pipe repair prize

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2001-04-01

    This paper describes the permanent pipeline repair system, developed by Tekmar, which is powered by seawater hydraulics and is easily installed and tested by any workclass remotely operated vehicle (rov). Details are given of the two main components of the system, namely, the diverless high pressure split repair clamp and the rov-operated tool to install it.

  3. Analysis list: Clamp [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Clamp Cell line + dm3 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/target/Clamp.1....tsv http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/target/Clamp.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/target/Cla...mp.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/colo/Clamp.Cell_line.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/colo/Cell_line.gml ...

  4. Upgrades in thermal protection for downhole instruments

    Energy Technology Data Exchange (ETDEWEB)

    Bennett, G.A.

    1985-01-01

    Measurement of geophysical parameters in progressively deeper and hotter wells has prompted design changes that improve the performance of downhole instruments and their associated thermal protection systems. This report provides a brief description of the mechanical and thermal loads to which these instruments and systems are subjected. Each design change made to the passive thermal protection system is described along with its resulting improvement. An outline of work being done to scope an active thermal protection system and the preliminary qualitative results are also described. 3 refs., 4 figs.

  5. Dynamics of Open DNA Sliding Clamps.

    Directory of Open Access Journals (Sweden)

    Aaron J Oakley

    Full Text Available A range of enzymes in DNA replication and repair bind to DNA-clamps: torus-shaped proteins that encircle double-stranded DNA and act as mobile tethers. Clamps from viruses (such as gp45 from the T4 bacteriophage and eukaryotes (PCNAs are homotrimers, each protomer containing two repeats of the DNA-clamp motif, while bacterial clamps (pol III β are homodimers, each protomer containing three DNA-clamp motifs. Clamps need to be flexible enough to allow opening and loading onto primed DNA by clamp loader complexes. Equilibrium and steered molecular dynamics simulations have been used to study DNA-clamp conformation in open and closed forms. The E. coli and PCNA clamps appear to prefer closed, planar conformations. Remarkably, gp45 appears to prefer an open right-handed spiral conformation in solution, in agreement with previously reported biophysical data. The structural preferences of DNA clamps in solution have implications for understanding the duty cycle of clamp-loaders.

  6. Loading dynamics of a sliding DNA clamp.

    KAUST Repository

    Cho, Won-Ki

    2014-05-22

    Sliding DNA clamps are loaded at a ss/dsDNA junction by a clamp loader that depends on ATP binding for clamp opening. Sequential ATP hydrolysis results in closure of the clamp so that it completely encircles and diffuses on dsDNA. We followed events during loading of an E. coli β clamp in real time by using single-molecule FRET (smFRET). Three successive FRET states were retained for 0.3 s, 0.7 s, and 9 min: Hydrolysis of the first ATP molecule by the γ clamp loader resulted in closure of the clamp in 0.3 s, and after 0.7 s in the closed conformation, the clamp was released to diffuse on the dsDNA for at least 9 min. An additional single-molecule polarization study revealed that the interfacial domain of the clamp rotated in plane by approximately 8° during clamp closure. The single-molecule polarization and FRET studies thus revealed the real-time dynamics of the ATP-hydrolysis-dependent 3D conformational change of the β clamp during loading at a ss/dsDNA junction.

  7. Multipurpose Acoustic Sensor for Downhole Fluid Monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Pantea, Cristian [Los Alamos National Laboratory

    2012-05-04

    The projects objectives and purpose are to: (1) development a multipurpose acoustic sensor for downhole fluid monitoring in Enhanced Geothermal Systems (EGS) reservoirs over typical ranges of pressures and temperatures and demonstrate its capabilities and performance for different EGS systems; (2) determine in real-time and in a single sensor package several parameters - temperature, pressure, fluid flow and fluid properties; (3) needed in nearly every phase of an EGS project, including Testing of Injection and Production Wells, Reservoir Validation, Inter-well Connectivity, Reservoir Scale Up and Reservoir Sustainability. (4) Current sensors are limited to operating at lower temperatures, but the need is for logging at high temperatures. The present project deals with the development of a novel acoustic-based sensor that can work at temperatures up to 374 C, in inhospitable environments.

  8. How Does Patch Clamp Work?--An easy approach to understanding patch clamp mechanism

    Institute of Scientific and Technical Information of China (English)

    关兵才; 李之望

    2003-01-01

    In order to help patch clamp users,most of whom are not good enough at electrics and electronics,to understandthe complicated principles of patch clamp technique,we present a readily accessible explanation of patch clamp mechanism,in-cluding basic analysis of membrane currents under voltage clamp,achievement of voltage clamp and current recording,capaci-tance compensation,series resistance compensation,voltage offset correction,leak subtraction and current clamp,based on ourworking and teaching experience and with the aid of some useful formulae.

  9. DOWNHOLE POWER GENERATION AND WIRELESS COMMUNICATIONS FOR INTELLIGENT COMPLETIONS APPLICATIONS

    Energy Technology Data Exchange (ETDEWEB)

    Paul Tubel

    2004-02-01

    The development work during this quarter was focused in the assembly of the downhole power generator hardware and its electronics module. The quarter was also spent in the development of the surface system electronics and software to extract the acoustic data transmitted from downhole to the surface from the noise generated by hydrocarbon flow in wells and to amplify very small acoustic signals to increase the distance between the downhole tool and the surface receiver. The tasks accomplished during this report period were: (1) Assembly of the downhole power generator mandrel for generation of electrical power due to flow in the wellbore. (2) Test the piezoelectric wafers to assure that they are performing properly prior to integrating them to the mechanical power generator mandrel. (3) Coat the power generator wafers to prevent water from shorting the power generator wafers. (4) Test of the power generator using a water tower and an electric pump to create a water flow loop. (5) Test the power harvesting electronics module. (6) Upgrade the signal condition and amplification from downhole into the surface system. (7) Upgrade the surface processing system capability to process data faster. (8) Create a new filtering technique to extract the signal from noise after the data from downhole is received at the surface system.

  10. Downhole Measurements of Shear- and Compression-Wave Velocities in Boreholes C4993, C4996, C4997 and C4998 at the Waste Treatment Plant DOE Hanford Site.

    Energy Technology Data Exchange (ETDEWEB)

    Redpath, Bruce B.

    2007-04-27

    This report describes the procedures and the results of a series of downhole measurements of shear- and compression-wave velocities performed as part of the Seismic Boreholes Project at the site of the Waste Treatment Plant (WTP). The measurements were made in several stages from October 2006 through early February 2007. Although some fieldwork was carried out in conjunction with the University of Texas at Austin (UT), all data acquired by UT personnel are reported separately by that organization.

  11. Protein folding in a force clamp

    Science.gov (United States)

    Cieplak, Marek; Szymczak, P.

    2006-05-01

    Kinetics of folding of a protein held in a force clamp are compared to an unconstrained folding. The comparison is made within a simple topology-based dynamical model of ubiquitin. We demonstrate that the experimentally observed variations in the end-to-end distance reflect microscopic events during folding. However, the folding scenarios in and out of the force clamp are distinct.

  12. Sound speed in downhole flow measurement.

    Science.gov (United States)

    Ünalmis, Ö Haldun

    2016-07-01

    This paper describes the use of sound speed in flow measurement applications in the high-pressure/high-temperature downhole environment. The propagation speed of a sound wave is a powerful tool to extract useful information from a flowing fluid medium in pipe whether the medium consists of a single-phase or multiphase flow. Considering the complex nature of the flow patterns and changing phase fractions from reservoir to surface, utilizing the propagation speed of sound of a fluid mixture is not a trivial task, especially if the interest is real-time flow measurement. The demanding applications span a wide spectrum from noisy medium originating from fast-moving gas/liquid flows to quiet medium originating from slow-moving liquid/liquid flows. In the current work, multiple flow loop tests are conducted in different facilities to evaluate the direct use of sound speed in flow rate measurement and the results are associated with real-life field examples. A tool analysis map is developed that addresses the use of sound speed for flow measurement under different scenarios. Although most examples are based on strain-based local sensing of the flow, the use of sound speed is independent of the methodology and can be implemented by other methods such as acoustic-based distributed sensing. PMID:27475167

  13. Dynamic clamp with StdpC software

    Science.gov (United States)

    2011-01-01

    Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting this current into the cell. Here, we present a dynamic clamp protocol using free, fully integrated, open-source software (StdpC, Spike timing dependent plasticity Clamp). Use of this protocol does not require specialist hardware, costly commercial software, experience in real time operating systems or a strong programming background. The software enables the configuration and operation of a wide range of complex and fully automated dynamic clamp experiments via an intuitive and powerful interface with a minimal initial lead-time of a few hours. After initial configuration, experimental results can be generated within minutes of cell impalement. PMID:21372819

  14. Inequalities for the Vibrating Clamped Plate Problem

    OpenAIRE

    MCHALE, K.P.

    2001-01-01

    We study the eigenvalues of the vibrating clamped plate problem. We have made improvements on the bounds of the ratios of the eigenvalues of the biharmonic operator (clamped plate) using the methods of Payne, Polya, and Weinberger. The difference in our proof lies mainly with the trial functions and the orthogonality arguments. While Payne, Polya, and Weinberger and Hile and Yeh project away components along u1,u2,...,uk to meet the orthogonality conditions,we use a translation/rotation ...

  15. Protein folding in a force-clamp

    Science.gov (United States)

    Cieplak, Marek; Szymczak, Piotr

    2006-03-01

    Kinetics of folding of a protein held in a force-clamp are compared to an unconstrained folding. The comparison is made within a simple topology-based dynamical model of ubiquitin. We demonstrate that the experimentally observed rapid changes in the end-to-end distance mirror microscopic events during folding. However, the folding scenarios in and out of the force-clamp are distinct.

  16. Protein folding in a force-clamp

    OpenAIRE

    Cieplak, Marek; Szymczak, Piotr

    2006-01-01

    Kinetics of folding of a protein held in a force-clamp are compared to an unconstrained folding. The comparison is made within a simple topology-based dynamical model of ubiquitin. We demonstrate that the experimentally observed variations in the end-to-end distance reflect microscopic events during folding. However, the folding scenarios in and out of the force-clamp are distinct.

  17. Piezoresistive cantilever force-clamp system

    Energy Technology Data Exchange (ETDEWEB)

    Park, Sung-Jin; Petzold, Bryan C.; Pruitt, Beth L. [Department of Mechanical Engineering, Stanford University, Stanford, California 94305 (United States); Goodman, Miriam B. [Department of Molecular and Cellular Physiology, Stanford University, Stanford, California 94305 (United States)

    2011-04-15

    We present a microelectromechanical device-based tool, namely, a force-clamp system that sets or ''clamps'' the scaled force and can apply designed loading profiles (e.g., constant, sinusoidal) of a desired magnitude. The system implements a piezoresistive cantilever as a force sensor and the built-in capacitive sensor of a piezoelectric actuator as a displacement sensor, such that sample indentation depth can be directly calculated from the force and displacement signals. A programmable real-time controller operating at 100 kHz feedback calculates the driving voltage of the actuator. The system has two distinct modes: a force-clamp mode that controls the force applied to a sample and a displacement-clamp mode that controls the moving distance of the actuator. We demonstrate that the system has a large dynamic range (sub-nN up to tens of {mu}N force and nm up to tens of {mu}m displacement) in both air and water, and excellent dynamic response (fast response time, <2 ms and large bandwidth, 1 Hz up to 1 kHz). In addition, the system has been specifically designed to be integrated with other instruments such as a microscope with patch-clamp electronics. We demonstrate the capabilities of the system by using it to calibrate the stiffness and sensitivity of an electrostatic actuator and to measure the mechanics of a living, freely moving Caenorhabditis elegans nematode.

  18. An Ultrasonic Clamp for Bloodless Partial Nephrectomy

    Science.gov (United States)

    Lafon, Cyril; Bouchoux, Guillaume; Murat, François Joseph; Birer, Alain; Theillère, Yves; Chapelon, Jean Yves; Cathignol, Dominique

    2007-05-01

    Maximum conservation of the kidney is preferable through partial nephrectomy for patients at risk of disease recurrence of renal cancers. Haemostatic tools are needed in order to achieve bloodless surgery and reduce post surgery morbidity. Two piezo-ceramic transducers operating at a frequency of 4 MHz were mounted on each arm of a clamp. When used for coagulation purposes, two transducers situated on opposite arms of the clamp were driven simultaneously. Heat delivery was optimized as each transducers mirrored back to targeted tissues the wave generated by the opposite transducer. Real-time treatment monitoring with an echo-based technique was also envisaged with this clamp. Therapy was periodically interrupted so one transducer could generate a pulse. The echo returning from the opposite transducer was treated. Coagulation necroses were obtained in vitro on substantial thicknesses (23-38mm) of pig liver over exposure durations ranging from 30s to 130s, and with acoustic intensities of less than 15W/cm2 per transducer. Both kidneys of two pigs were treated in vivo with the clamp (14.5W/cm2 for 90s), and the partial nephrectomies performed proved to be bloodless. In vitro and in vivo, wide transfixing lesions corresponded to an echo energy decrease superior to -10dB and parabolic form of the time of flight versus treatment time. In conclusion, this ultrasound clamp has proven to be an excellent mean for achieving monitored haemostasis in kidney.

  19. IMPORTANT: Fluke is recalling Digital Clamp Meters

    CERN Multimedia

    2013-01-01

    Fluke is voluntarily recalling four models of Digital Clamp Meters: Fluke 373, 374, 375 and 376. If you own one of these clamp meters, please stop using it and send it back to Fluke for repair even if you have not experienced problems.   Description of the problem: "The printed circuit assembly may not be properly fastened to the test lead input jack. This may result in inaccurate voltage readings, including a low or no-voltage reading on a circuit energised with a hazardous voltage, presenting a shock, electrocution or thermal burn hazard." To determine if your clamp meter is affected by this recall notice, and for more information, click here.

  20. Design and application of multifunctional stomach clamp

    Institute of Scientific and Technical Information of China (English)

    Yun Fu Lu; Pi Wu Li; Xir Xin Zhang

    2000-01-01

    AIM Pylorus and pyloric vagus preserving gastrectomy (PPVPG) is an efficient operation mode for treatinggastric and duodenal ulcers. For its better application and popularization, we have designed andmanufactured a special multifunctional stomach clamp.METHODS The clamp has been designed carefully in view of the different sizes of the patients' stomachsand the different starting points of Latarjer nerves so as to facilitate the execution of the present operationmode, which requires the preservation of the vagus innervated pyloric region so as to make the remnantstomach maintain its normal physiological function and the vagus branches clinging antral seromuscular flapkept in proper size so that avoiding too much remaining antral mucosa is removed to cause greater difficultyin operation. With this in view, we observed and measured the distance between the Latarjer nerves distribution and the pyloric rings of 42 patients' stomachs in the course of operations. We bent an aluminumstring 3 mm thick for each stomach according to the size of antral seromuecular flap and chose an optimalcurvature from the 42 sizes for manufacturing the clamp. The clamp is made of 3 Cr13 stainless steel,consisting of three parts, the curved head, the fastening ring and the handle. The curved head is shapedalmost exactly like the remained antral seromuscular flap. The ring at its tip when in place fastens the twosides of the clamp so that the tissue of the stomach between will be clutched by an even force. On one sideare three cuts for moving the ring upwards, each cut representing 1 mm for fastening and adjustments can bemade in view of the thickness or thinness of the tissue. The handles bend backward for fighting shy of thecuts so as to facilitate the operation.RESULTS With the stomach disassociated in the light of PPVPG procedures, the clamp may easily beapplied from the upper part of the Latarjer nerves. The following merits can be noted. ① The curving line ofthe flap can be precisely

  1. Prolonged penile strangulation with metal clamps

    Institute of Scientific and Technical Information of China (English)

    Chirag Patel; Richard Kim; Michael Delterzo; Run Wang

    2006-01-01

    Various different objects have been reported to strangulate the penis. We reported on a patient who used metal radiator clamps for an extended period of time. Workup included history, physical examination and urinalysis. The patient was taken to the operating room for further evaluation with cystourethroscopy and orthopedic wire cutters were used to break the metal bands.

  2. Seismic testing

    International Nuclear Information System (INIS)

    This lecture deals with: qualification methods for seismic testing; objectives of seismic testing; seismic testing standards including examples; main content of standard; testing means; and some important elements of seismic testing

  3. Unlocking thin bed potential: gas identification through downhole fluid analysis

    Energy Technology Data Exchange (ETDEWEB)

    Loi, D.; Mazzoni, S.; Gigliotti, M.; Baio, C.; Borghi, M.; Baldini, D.; Italiano, F. [Schlumberger (United States)

    2011-07-01

    Thin beds have been recently proposed as an alternative to multilayer sand reservoirs for countering the decline in production from offshore gas fields. However, this approach is still not applied because of difficulties in identifying gas bearing zones. This paper presents a critical review of existing thin bed evaluation techniques and describes a successful case history in the Adriatic sea in which wireline formation testing (RFT) technology, using a dual packer string with downhole fluid analysis capabilities, including fluid density measurement, was used to distinguish gas zones from water zones. This approach gave a 100% success ratio for downhole fluid analysis stations in a tight environment and provided an effective way to acquire reservoir pressure without supercharging effects and allow the operator to confirm gas presence in several thin bed levels. Moreover, this approach also helps to improve optimization of completion techniques (FracPack and High Rate Water Pack) and saves the cost of multiple well testings, thus reducing total costs.

  4. Research on Overflow Monitoring Mechanism Based on Downhole Microflow Detection

    Directory of Open Access Journals (Sweden)

    Liang Ge

    2014-01-01

    Full Text Available The flow rate variation of the drilling fluid and micro-overflow loss is difficult to analyze. The purpose to prevent the occurrence of kick, lost circulation, and other complex conditions is not easy to be achieved. Therefore, the microflow-induced annulus multiphase flow rate and annulus pressure field model were studied, and a downhole microflow measurement system has been developed. A differential pressure type flow measurement was used in the system, and real-time downhole information was obtained to achieve deep, narrow windows and other safety-density complex formation security. This paper introduced a new bottom-hole flow meter which can measure the annular flux while drilling and monitor overflow and circulation loss. The accuracy and reliability of the MPD (managed pressure drilling system can be improved obviously by applying the device; as a result, the safety of drilling is enhanced and the cost is reduced.

  5. Active cooling for downhole instrumentation: Preliminary analysis and system selection

    Energy Technology Data Exchange (ETDEWEB)

    Bennett, G.A.

    1988-03-01

    A feasibility study and a series of preliminary designs and analyses were done to identify candidate processes or cycles for use in active cooling systems for downhole electronic instruments. A matrix of energy types and their possible combinations was developed and the energy conversion process for each pari was identified. The feasibility study revealed conventional as well as unconventional processes and possible refrigerants and identified parameters needing further clarifications. A conceptual design or series od oesigns for each system was formulated and a preliminary analysis of each design was completed. The resulting coefficient of performance for each system was compared with the Carnot COP and all systems were ranked by decreasing COP. The system showing the best combination of COP, exchangeability to other operating conditions, failure mode, and system serviceability is chosen for use as a downhole refrigerator. 85 refs., 48 figs., 33 tabs.

  6. A robust economic technique for crosswell seismic profiling. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Hardage, B.A.; Simmons, J.L. Jr.

    1998-01-01

    The objective of this research program was to investigate a novel way to acquire crosswell tomographic data, that being to use a standard surface-positioned seismic energy source stationed inline with two wells that have downhole receiver arrays. This field technique differs from the traditional way that crosswell tomography is done, which requires that a downhole receiver array be in one well and that a downhole seismic source be in a second well. The purpose of the research effort was to evaluate the relative merits of the potential advantages and pitfalls of surface-source crosswell tomography, which some also refer to as twin-receiver-well crosswell tomography. The principal findings were: (1) surface-source crosswell tomography is a viable technology and can be used in appropriate reservoir conditions, (2) raypath modeling should be done to determine if the targeted interwell space is properly illuminated by surface-generated wavefields before proceeding to collect surface-source tomographic data, (3) crosswell data generated by a surface-based source are subject to a greater range of traveltime errors than are data generated by a downhole source, primarily due to shot statics caused by variable weathered layers, and (4) the accuracy and reliability of the interwell tomogram increase as more independent velocity information (sonic logs, velocity checkshots, vertical seismic profiles, downhole-source crosswell data) is available to constrain the inversion. The surface-source approach to crosswell tomography was evaluated by recording twin-receiver well data at the Texaco Borehole Test Site in Humble, Texas.

  7. Mechanical Analysis of the Down-Hole String in a Deep Well

    Institute of Scientific and Technical Information of China (English)

    HE Yu-fa; LIU Qing-you; ZHU Xiao-lan

    2010-01-01

    In a deep well, under high pressure and temperature, the force, deformation, and movement of the downhole string are very complicated. In this paper, based on the analysis of the working environment and load properties of the down-hole string in deep wells, the factors of high temperature, high pressure, and the complex structure of wells have been taken into consideration. The loads of the down-hole string, stability, back buckle configuration, dynamic characteristics, mechanical strength, and deformation have been systematically studied. On this basis, mechanical models of a down-hole string under different conditions have been established and software for aiding analysis has been developed. By using this software, deep well strings under different construction parameters can be simulated and analyzed, providing a theoretical basis and method for down-hole string assembly, as well as string down-hole construction and operation.

  8. Fate of hydraulic fracturing chemicals under down-hole conditions

    Science.gov (United States)

    Blotevogel, J.; Kahrilas, G.; Corrin, E. R.; Borch, T.

    2013-12-01

    Hydraulic fracturing is a method to increase the yield of oil and natural gas extraction from unconventional rock formations. The process of hydrofracturing occurs via injecting water, sand, and chemicals into the production well and subjecting this mixture to high pressures to crack the rock shale, allowing increased amounts of gas and oil to seep out of the target formation. Typical constituents of the chemical mixtures are biocides, which are applied to inhibit growth of sulfate reducing bacteria in order to prevent pipe corrosion and production of hazardous gases. However, very little is known about the persistence, fate, and activity of biocides when subjected to the high temperatures and pressures of down-hole conditions. Thus, the objective of this talk is to present data from ongoing experiments focused on determining the fate of biocides commonly used for hydraulic fracturing under conditions simulating down-hole environments. Using stainless steel reactors, the high pressures and temperatures of down-hole conditions in the Marcellus shale are simulated, while concentration, speciation, and degradation of priority biocides are observed as a function of time, using primarily LC/MS techniques. The impact of water quality, shale, temperature, and pressure on the transformation kinetics and pathways of biocides will be discussed. Finally, field samples (both sediments and flowback brine) from the Marcellus shale are analyzed to verify that our lab simulations mirror real-life conditions and results.

  9. Multilevel-clamped multilevel converters (MLC2)

    DEFF Research Database (Denmark)

    Rodriguez, Pedro; Bellar, Maria Dias; Muñoz-Aguliar, Raúl Santiago; Busquets-Monge, Sergio; Blaabjerg, Frede

    2012-01-01

    This letter introduces a new series of multilevel (ML) converters based on the ML clamping concept. By applying this technique, a ML clamping unit (MCU) conveys additional levels for synthesizing the output waveforms of a diode-clampedML dc– ac power converter. The basic building block of the ML ...

  10. Γ-source Neutral Point Clamped Inverter

    DEFF Research Database (Denmark)

    Mo, Wei; Loh, Poh Chiang; Blaabjerg, Frede

    Transformer based Z-source inverters are recently proposed to achieve promising buck-boost capability. They have improved higher buck-boost capability, smaller size and less components count over Z-source inverters. On the other hand, neutral point clamped inverters have less switching stress and...... better output performance comparing with traditional two-level inverters. Integrating these two types of configurations can help neutral point inverters achieve enhanced votlage buck-boost capability....

  11. π-Clamp-mediated cysteine conjugation

    Science.gov (United States)

    Zhang, Chi; Welborn, Matthew; Zhu, Tianyu; Yang, Nicole J.; Santos, Michael S.; van Voorhis, Troy; Pentelute, Bradley L.

    2016-02-01

    Site-selective functionalization of complex molecules is one of the most significant challenges in chemistry. Typically, protecting groups or catalysts must be used to enable the selective modification of one site among many that are similarly reactive, and general strategies that selectively tune the local chemical environment around a target site are rare. Here, we show a four-amino-acid sequence (Phe-Cys-Pro-Phe), which we call the ‘π-clamp’, that tunes the reactivity of its cysteine thiol for site-selective conjugation with perfluoroaromatic reagents. We use the π-clamp to selectively modify one cysteine site in proteins containing multiple endogenous cysteine residues. These examples include antibodies and cysteine-based enzymes that would be difficult to modify selectively using standard cysteine-based methods. Antibodies modified using the π-clamp retained binding affinity to their targets, enabling the synthesis of site-specific antibody-drug conjugates for selective killing of HER2-positive breast cancer cells. The π-clamp is an unexpected approach to mediate site-selective chemistry and provides new avenues to modify biomolecules for research and therapeutics.

  12. Hyperinsulinemic Euglycemic clamp for cardiac PET

    International Nuclear Information System (INIS)

    The aim of this study was to assess the adequacy of Hyperinsulinemic Euglycaemic clamp to yield technically adequate and clinically meaningful cardiac scans. The F- 18 Fluorodeoxyglucose scans obtained in diabetic patients are of sub-optimal quality as high level of glucose competes with radioactive Fluorodeoxyglucose for cardiac uptake. Thus to allow quick stabilization of metabolic environment and to get superior quality images, insulin clamp is an accepted technique. Thirty patients (20 males and 5 females) with ages ranging from 43 to 69 years, who were referred for post-myocardial infarction estimation of myocardial viability, were evaluated in this study. All patients reported for the test after 6 hours of fasting. At first myocardial perfusion studies were carried out in all patients with Tc-99m Tetrofosmin on a GE dual head gamma camera. Blood glucose levels were checked. The blood sugar levels in the patients were in the range of 130 - 190 mg%. Insulin clamp was applied depending upon the glucose level obtained, according to ASNC guidelines for PET Myocardial Glucose Metabolism and Perfusion Imaging. On stabilization of blood glucose levels, 10 mCi of F-18 FDG was injected and imaging was performed at 1 hour on a dedicated 16 slice STE GE PET -- CT scanner. Three patients had incidence of hypoglycemia as a side effect during the procedure, their blood sugar falling to 40 mg%. They were treated with 25ml of 25% dextrose intravenously following which there was stabilization of blood sugar. Entire procedure including Tetrofosmin imaging took approximately 4-6 hours. Good quality images were obtained after the use of insulin clamp. Infusion of insulin and glucose gives stable plasma glucose levels during imaging. The insulin clamp technique makes it possible to adjust and maintain a metabolic steady state during the PET study. It does not alter F-18 FDG uptake patterns in different myocardial areas and gives superior quality images. The technique is safe and

  13. Condition of chromic acid anodized aluminum clamps flown

    Science.gov (United States)

    Plagemann, W. L.

    1991-01-01

    A survey of the condition of the chromic acid anodized (CAA) coating on selected LDEF tray clamps was carried out. Measurements of solar absorptance and thermal emittance were carried out at multiple locations on both the space exposed and spacecraft facing sides of the clamps. Multiple clamps from each available angle relative to the ram direction were examined. The diffuse component of the reflectance spectrum was measured for a selected subset of the clamps. The thickness of the CAA was determined for a small set of clamps. Examples of variation in integrity of the coatings from leading to trailing edge will be shown.

  14. Downhole cuttings injection allows use of oil-base muds

    International Nuclear Information System (INIS)

    This paper reports that of the potential methods for handling oily drill cuttings, the most attractive is their injection downhole. This approach, which has been used by BP on its Gyda platform in the North Sea where stringent new environmental regulations are expected, will enable operators to enjoy the economic advantages of using oil-based muds. The discharge of oil-based-mud-contaminated cuttings form offshore drilling operations has a significant, though localized, environmental impact. This is despite the change from diesel-based fluids to less toxic, low aromatic, base oils which occurred in the late 1970s

  15. Biological cell controllable patch-clamp microchip

    Science.gov (United States)

    Penmetsa, Siva; Nagrajan, Krithika; Gong, Zhongcheng; Mills, David; Que, Long

    2010-12-01

    A patch-clamp (PC) microchip with cell sorting and positioning functions is reported, which can avoid drawbacks of random cell selection or positioning for a PC microchip. The cell sorting and positioning are enabled by air bubble (AB) actuators. AB actuators are pneumatic actuators, in which air pressure is generated by microheaters within sealed microchambers. The sorting, positioning, and capturing of 3T3 cells by this type of microchip have been demonstrated. Using human breast cancer cells MDA-MB-231 as the model, experiments have been demonstrated by this microchip as a label-free technical platform for real-time monitoring of the cell viability.

  16. Design of a hot clamp for small diameter LMFBR piping

    International Nuclear Information System (INIS)

    In order to evaluate the feasibility of using a strap-type clamp on breeder reactor small diameter piping, a series of analyses were conducted to determine the thermal and mechanical stresses induced in the pipe by the clamp. The effect of using a thin layer of insulation of varying thickness between the pipe and clamp was also investigated. A thermal analysis of the pipe and clamp was conducted for each thermal transient rate. Various insulation values ranging from metal-to-metal condition to perfect insulation between the pipe and clamp were analyzed to determine the effect of insulating the pipe from the clamp. An axisymmetric finite element model was used to determine the axial thermal and stress patterns

  17. Results from Downhole Logging in the ICDP Project Scopsco at Lake Ohrid (Macedonia, Albania)

    Science.gov (United States)

    Baumgarten, H.; Wonik, T.; Wagner, B.

    2014-12-01

    Lake Ohrid is located at the border between Macedonia and Albania (40°70' N, 20°42 E) and is assumed as the oldest lake in Europe. The lake with a surface area of 360 km² has trapped sediments and volcanic ashes and hence, contains essential information of major climatic and environmental change of the central northern Mediterranean region. In the frame of the ICDP project SCOPSCO (Scientific Collaboration on Past Speciation Conditions in Lake Ohrid), several scientific questions are adressed: age and origin of the lake, paleoclimatic change during the Quaternary, tephrostratigraphy, and driving forces for the outstanding biodiversity. Sediments of the lake have been targeted for a deep drilling campaign in spring 2013. Four sites (DEEP, CERAVA, GRADISTE, PESTANI) have been cored to a depth of 569 m below lake floor (blf). High-quality continuous downhole logging data have been achieved at all drill sites by the use of the following tools: spectral gamma ray, magnetic susceptibility, resistivity, dipmeter, borehole televiewer and sonic. Additionally, vertical seismic profiling was conducted at the DEEP site. Seismic investigations indicate a sediment fill of the lake basin up to a thickness of 700 m. First results from cores revealed, that the bottom part, below 430 m blf is characterized by coarser grained deposits while the upper part yields fine grained pelagic sediments. The borehole logging data at the DEEP site shows strong contrasts in all physical properties, in particular in spectral gamma ray (GR), magnetic susceptibility, resistivity and seismic velocity (vp). Furthermore, the pelagic facies show a cyclic alternation in these parameters as well as in the structural features from borehole televiewer. By use of the current age estimate of the record of around 1.2 Ma, the GR data was tested for matching with the benthic δ18O record and shows high correlation (R² = 0.72), whereas high GR indicate glacial and low GR interglacial periods. Spectral

  18. PROGRESS REPORT. ADVANCED HIGH RESOLUTION SEISMIC IMAGING, MATERIAL PROPERTIES ESTIMATION AND FULL WAVEFIELD INVERSION FOR THE SHALLOW SUBSURFACE

    Science.gov (United States)

    In July and August 2000, we conducted 3-D reflection, tomography, and downhole seismic studies at Operable Unit 2 (OU2) at Hill Air Force Base (HAFB) in Ogden, Utah. OU2 has been the subject of ongoing remediation efforts to remove dense nonaqueous phase liquids (DNAPLs) that con...

  19. Monitoring hydraulic fracturing with seismic emission volume

    Science.gov (United States)

    Niu, F.; Tang, Y.; Chen, H.; TAO, K.; Levander, A.

    2014-12-01

    Recent developments in horizontal drilling and hydraulic fracturing have made it possible to access the reservoirs that are not available for massive production in the past. Hydraulic fracturing is designed to enhance rock permeability and reservoir drainage through the creation of fracture networks. Microseismic monitoring has been proven to be an effective and valuable technology to image hydraulic fracture geometry. Based on data acquisition, seismic monitoring techniques have been divided into two categories: downhole and surface monitoring. Surface monitoring is challenging because of the extremely low signal-to-noise ratio of the raw data. We applied the techniques used in earthquake seismology and developed an integrated monitoring system for mapping hydraulic fractures. The system consists of 20 to 30 state-of-the-art broadband seismographs, which are generally about hundreds times more sensible than regular geophones. We have conducted two experiments in two basins with very different geology and formation mechanism in China. In each case, we observed clear microseismic events, which may correspond to the induced seismicity directly associated with fracturing and the triggered ones at pre-existing faults. However, the magnitude of these events is generally larger than magnitude -1, approximately one to two magnitudes larger than those detected by downhole instruments. Spectrum-frequency analysis of the continuous surface recordings indicated high seismic energy associated with injection stages. The seismic energy can be back-projected to a volume that surrounds each injection stage. Imaging seismic emission volume (SEV) appears to be an effective way to map the stimulated reservior volume, as well as natural fractures.

  20. 275 C Downhole Switched-Mode Power Supply

    Energy Technology Data Exchange (ETDEWEB)

    Chris Hutchens; Vijay Madhuravasal

    2008-08-31

    A vee-square (V2) control based controller IC is developed for a switch mode power supply capable of operating at extreme temperature/harsh environment conditions. A buck type regulator with silicon carbide power junction field effect transistors (JFET) as power devices is used to analyze the performance of controller. Special emphases are made on the analog sub-blocks--voltage reference, operational transconductance amplifier and comparator as individual building blocks. Transformer coupled gate drives and high temperature operable magnetic cores and capacitors are identified and tested for use in the design. Conventional ceramic chip packaging of ICs combined with lead carrier type mounting of passive filter components is introduced for hybrid packaging of the complete product. The developed SMPS is anticipated to support the operation of down-hole microcontrollers and other electronics devices that require low/medium power filtered dc inputs over an operating temperature of 275 C.

  1. DOWNHOLE POWER GENERATION AND WIRELESS COMMUNICATIONS FOR INTELLIGENT COMPLETIONS APPLICATIONS

    Energy Technology Data Exchange (ETDEWEB)

    Paul Tubel

    2003-10-14

    The fourth quarter of the project was dedicated to the manufacturing of the mechanical system for wireless communications and the power generation module and inspection pre assembly of the mechanical components. Another emphasis for the quarter was the development of filter control and signal detection software. The tasks accomplished during this report period were: (1) Dimensional issues were resolved and revised drawings for manufacturing of the wireless communications gauge and power generator were completed and sent to a machine shop for manufacturing. (2) Finalized the requirements and fittings and connections for testing the tool in the Halliburton flow loop. (3) The new acoustic generator was manufactured successfully and it was delivered during this quarter. The assembly will be outsourced for plastic coating in preparation for hostile environment use. (4) The acoustic two-way communications development continued to progress. The real time firmware for the surface system was developed and the processor was able to detect and process the data frame transmitted from downhole. The analog section of the tool was also developed and it is being tested for filtering capabilities and signal detection and amplification. (5) The new transformer to drive the acoustic generator assembly was manufactured and was successfully tested. Spring mandrel design showed increased acoustic output on the pipe and was implemented. (6) PCBA board carrier with board set was tested for function and fit and is 100% complete. (7) Filter control software is complete and software to allow modification of communication parameters dynamically is 50% complete. (8) All mechanical parts to assemble the wireless gauge and power generator have been received and verified to be within specification. (9) Acoustic generator has been assembled in the tool mandrel and tested successfully. (10) The circuit required to harvest the power generated downhole has been designed and the power generator

  2. The role of duplex stainless steels for downhole tubulars

    International Nuclear Information System (INIS)

    In sour conditions there is an increasing trend to turn to corrosion resistant alloys for downhole tubulars. The most commonly used CRA tubular is 13Cr, and there are thousands of feet in service. However, there are limits to the use of 13Cr, ie., the risk of sulphide stress corrosion cracking at high H2S levels, and the possibility of pitting or high corrosion rates in waters with high chloride contents. Where the service conditions are felt to be too severe for 13Cr alloys it has been traditional to switch to nickel base alloys such as alloys 825 and C-276 (UNS N08825 and N10276). The alloys are much more expensive than 13Cr, and in recent years the duplex stainless steels have been selected as alloys with superior corrosion and SSCC resistance compared with 13Cr, and having lower cost than nickel alloys. Originally the 22Cr duplex alloy (UNS 31803) was used, but more recently the 25Cr super duplex alloys (UNS S32760 and S32750) have become more available. The present paper reviews the data available for 13Cr and the limits of applicability. Data is also presented for laboratory tests for both the 22Cr and 25Cr super duplex alloys. There is extensive service experience with both 22Cr and 25Cr super duplex in the North Sea, covering both downhole tubulars, manifold and post wellhead equipment. Data is presented showing some of the sour condition being experienced in the North Sea by super duplex alloys. These results show that there is a substantial gap between the limits of use for 13Cr and the 25Cr super duplex stainless steel alloys. This means that in many sour environments super duplex stainless steel provides a cost effective alternative to nickel-base alloys

  3. Combination Space Station Handrail Clamp and Pointing Device

    Science.gov (United States)

    Hughes, Stephen J. (Inventor)

    1999-01-01

    A device for attaching an experiment carrier to a space station handrail is provided. The device has two major components, a clamping mechanism for attachment to a space station handrail, and a pointing carrier on which an experiment package can be mounted and oriented. The handrail clamp uses an overcenter mechanism and the carrier mechanism uses an adjustable preload ball and socket for carrier positioning. The handrail clamp uses a stack of disk springs to provide a spring loaded button. This configuration provides consistent clamping force over a range of possible handrail thicknesses. Three load points are incorporated in the clamping mechanism thereby spreading the clamping load onto three separate points on the handrail. A four bar linkage is used to provide for a single actuation lever for all three load points. For additional safety, a secondary lock consisting of a capture plate and push lock keeps the clamp attached to the handrail in the event of main clamp failure. For the carrier positioning mechanism, a ball in a spring loaded socket uses friction to provide locking torque; however. the ball and socket are torque limited so that the ball ran slip under kick loads (125 pounds or greater). A lead screw attached to disk spring stacks is used to provide an adjustable spring force on the socket. A locking knob is attached to the lead screw to allow for hand manipulation of the lead screw.

  4. New active load voltage clamp for HF-link converters

    DEFF Research Database (Denmark)

    Ljusev, Petar; Andersen, Michael Andreas E.

    2005-01-01

    This paper proposes a new active clamp for HF-link converters, which features very high efficiency by returning the clamped energy back to the primary side through a small auxiliary converter. It also increases the reliability of HF-link converters by providing an alternative load current path...

  5. Analysis of Contact Characteristic of Overhead Line and Suspension Clamp

    Directory of Open Access Journals (Sweden)

    Zhao Xinze

    2013-01-01

    Full Text Available In this paper, a LGJ150/25 type ACSR transmission line and a CGU-3 type suspension clamp are taken as research objects. A contact model of the conductor and the clamp was established by using finite element method. The effects of sag angle of the conductor, holding force and tension force in section are analyzed. The results showed that the contact area in the middle of the clamp is of belt-like type. The extreme values of tress were observed on the edge of the contact area and near the edge of keeper. In clamp section, suspension angle had the greatest influence on contact stress, and then the clamp force. The tension force in section played a most important role in these affecting factors. In the exit section of clamp, the biggest impact factor was tension force in this section, then the suspension angle, the third was clamp force. The results provide theoretical basis on reducing corona loss, optimization the clamp. Doubtlessly, the conclusion has important theoretical significance and application value.

  6. 21 CFR 876.5160 - Urological clamp for males.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Urological clamp for males. 876.5160 Section 876.5160 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES GASTROENTEROLOGY-UROLOGY DEVICES Therapeutic Devices § 876.5160 Urological clamp for...

  7. Applications of alcohol clamping in early drug development

    NARCIS (Netherlands)

    Zoethout, Remco Wiebe Martijn

    2012-01-01

    This thesis describes the development of a novel alcohol clamp, a new method to obtain stable plasma levels of alcohol and its application in CNS-research. The method might have several advantages that were explored in subsequent studies described in this thesis. The stability of the alcohol clamp w

  8. Program to develop improved downhole drilling motors. Semi-annual report

    Energy Technology Data Exchange (ETDEWEB)

    Maurer, W.C.

    1976-11-01

    Research progress is reported in two main sections: downhole drilling motor design and design of downhole motor seal, bearing, and test lubrication systems. Information on downhole drilling motor design is presented under the following subsection headings: Turbodrill development; positive displacement motor development; theory of Turbodrills; theory of positive displacement motors; basic motor components; forces on thrust bearings; thrust bearing design; radial bearing design; rotary seal design; sealed lubrication system; lubricants; and project status. The appendix contains information on high temperature lubricants. Information on the design of downhole motor seal, bearing, and test lubrication systems is presented under the following subsection headings: seal, bearing, and lubrication test design criteria; basic test conditions; independent and dependent variable parameters; alternative concepts for seal test apparatus; design of the seal test system; and bearing test system description. A schedule for remaining tasks is included. (JGB)

  9. A new method for downhole testing of oil- and gas wells entails less environmentally damaging emissions

    International Nuclear Information System (INIS)

    This very brief article deals with a new downhole test method for oil- and gas wells that will require less flaring and cause less air pollution. The method was developed jointly by Statoil, Shell, Petrotech and Halliburton

  10. Investigation of Cogging Torque of a Downhole Drilling Machine and Optimization of Electrical Machine

    OpenAIRE

    Ullah, Aasim

    2012-01-01

    This paper is a report on master thesis project conducted in cooperation with SMARTMOTOR AS and NTNU. The research for electrical downhole drilling machine has developed for decades. Permanent Magnet is a new addition in this arena. SMARTMOTOR AS built a downhole drilling machine which is needed to compare with machines from other competitors of the market. This machine has 10% cogging torque over its rated torque. The reason of this cogging torque and possible solutions has been investigated...

  11. Delay Pressure Detection Method to Eliminate Pump Pressure Interference on the Downhole Mud Pressure Signals

    OpenAIRE

    Yue Shen; Ling-Tan Zhang; Shi-Li Cui; Li-Min Sheng; Lin Li; Yi-Nao Su

    2013-01-01

    The feasibility of applying delay pressure detection method to eliminate mud pump pressure interference on the downhole mud pressure signals is studied. Two pressure sensors mounted on the mud pipe in some distance apart are provided to detect the downhole mud continuous pressure wave signals on the surface according to the delayed time produced by mud pressure wave transmitting between the two sensors. A mathematical model of delay pressure detection is built by analysis of transmission path...

  12. Surface-downhole electrical resistivity tomography applied to monitoring of the CO2 storage Ketzin (Germany)

    OpenAIRE

    Peter Bergmann; Cornelia Schmidt-Hattenberger; Kiessling, D.; Rücker, C.; Tim Labitzke; Jan Henninges; Gunther Baumann; Schütt, H.;  

    2012-01-01

    Surface-downhole electrical resistivity tomography (SDERT) surveys were repeatedly carried out to image CO2 injected at the pilot storage Ketzin, Germany. The experimental setup combines surface with downhole measurements by using a permanent electrode array that has been deployed in three wells. We performed one baseline and three repeat experiments; the first survey was performed during the site startup and the subsequent surveys during the first year of CO2 injection. By the time of the th...

  13. Deghosting, Demultiple, and Deblurring in Controlled-Source Seismic Interferometry

    Directory of Open Access Journals (Sweden)

    Joost van der Neut

    2011-01-01

    Full Text Available With controlled-source seismic interferometry we aim to redatum sources to downhole receiver locations without requiring a velocity model. Interferometry is generally based on a source integral over cross-correlation (CC pairs of full, perturbed (time-gated, or decomposed wavefields. We provide an overview of ghosts, multiples, and spatial blurring effects that can occur for different types of interferometry. We show that replacing cross-correlation by multidimensional deconvolution (MDD can deghost, demultiple, and deblur retrieved data. We derive and analyze MDD for perturbed and decomposed wavefields. An interferometric point spread function (PSF is introduced that can be obtained directly from downhole data. Ghosts, multiples, and blurring effects that may populate the retrieved gathers can be locally diagnosed with the PSF. MDD of perturbed fields can remove ghosts and deblur retrieved data, but it leaves particular multiples in place. To remove all overburden-related effects, MDD of decomposed fields should be applied.

  14. Dynamic clamp: a powerful tool in cardiac electrophysiology.

    Science.gov (United States)

    Wilders, Ronald

    2006-10-15

    Dynamic clamp is a collection of closely related techniques that have been employed in cardiac electrophysiology to provide direct answers to numerous research questions regarding basic cellular mechanisms of action potential formation, action potential transfer and action potential synchronization in health and disease. Building on traditional current clamp, dynamic clamp was initially used to create virtual gap junctions between isolated myocytes. More recent applications include the embedding of a real pacemaking myocyte in a simulated network of atrial or ventricular cells and the insertion of virtual ion channels, either simulated in real time or simultaneously recorded from an expression system, into the membrane of an isolated myocyte. These applications have proven that dynamic clamp, which is characterized by the real-time evaluation and injection of simulated membrane current, is a powerful tool in cardiac electrophysiology. Here, each of the three different experimental configurations used in cardiac electrophysiology is reviewed. Also, directions are given for the implementation of dynamic clamp in the cardiac electrophysiology laboratory. With the growing interest in the application of dynamic clamp in cardiac electrophysiology, it is anticipated that dynamic clamp will also prove to be a powerful tool in basic research on biological pacemakers and in identification of specific ion channels as targets for drug development. PMID:16873403

  15. POLYCYTHEMIA RISK IN NEONATES WITH DELAYED CORD CLAMPING

    OpenAIRE

    I Gusti Agung Ayu Sri Wulandari Pramana; Ketut Putera Kemara; I Wayan Megadhana

    2013-01-01

    Delayed Cord Clamping is still a controversial issue in the world of medicine. Until recently, the perfect times to do a Delayed Cord Clamping in various parts of the Earth in the world are still very diverse. There are a few things that became a major concern associated with Delayed Cord Clamping, one of which is the increase in blood volume due to an increase in the number of erythrocytes which led to occurrence of neonatal polycythemia which later can lead to various complications. Polycy...

  16. Advanced Seismic While Drilling System

    Energy Technology Data Exchange (ETDEWEB)

    Robert Radtke; John Fontenot; David Glowka; Robert Stokes; Jeffery Sutherland; Ron Evans; Jim Musser

    2008-06-30

    . An APS Turbine Alternator powered the SeismicPULSER{trademark} to produce two Hz frequency peak signals repeated every 20 seconds. Since the ION Geophysical, Inc. (ION) seismic survey surface recording system was designed to detect a minimum downhole signal of three Hz, successful performance was confirmed with a 5.3 Hz recording with the pumps running. The two Hz signal generated by the sparker was modulated with the 3.3 Hz signal produced by the mud pumps to create an intense 5.3 Hz peak frequency signal. The low frequency sparker source is ultimately capable of generating selectable peak frequencies of 1 to 40 Hz with high-frequency spectra content to 10 kHz. The lower frequencies and, perhaps, low-frequency sweeps, are needed to achieve sufficient range and resolution for realtime imaging in deep (15,000 ft+), high-temperature (150 C) wells for (a) geosteering, (b) accurate seismic hole depth, (c) accurate pore pressure determinations ahead of the bit, (d) near wellbore diagnostics with a downhole receiver and wired drill pipe, and (e) reservoir model verification. Furthermore, the pressure of the sparker bubble will disintegrate rock resulting in an increased overall rates of penetration. Other applications for the SeismicPULSER{trademark} technology are to deploy a low-frequency source for greater range on a wireline for Reverse Vertical Seismic Profiling (RVSP) and Cross-Well Tomography. Commercialization of the technology is being undertaken by first contacting stakeholders to define the value proposition for rig site services utilizing SeismicPULSER{trademark} technologies. Stakeholders include national oil companies, independent oil companies, independents, service companies, and commercial investors. Service companies will introduce a new Drill Bit SWD service for deep HTHP wells. Collaboration will be encouraged between stakeholders in the form of joint industry projects to develop prototype tools and initial field trials. No barriers have been identified

  17. Downhole temperature tool accurately measures well bore profile

    International Nuclear Information System (INIS)

    This paper reports that an inexpensive temperature tool provides accurate temperatures measurements during drilling operations for better design of cement jobs, workovers, well stimulation, and well bore hydraulics. Valid temperature data during specific wellbore operations can improve initial job design, fluid testing, and slurry placement, ultimately enhancing well bore performance. This improvement applies to cement slurries, breaker activation for slurries, breaker activation for stimulation and profile control, and fluid rheological properties for all downhole operations. The temperature tool has been run standalone mounted inside drill pipe, on slick wire line and braided cable, and as a free-falltool. It has also been run piggyback on both directional surveys (slick line and free-fall) and standard logging runs. This temperature measuring system has been used extensively in field well bores to depths of 20,000 ft. The temperature tool is completely reusable in the field, ever similar to the standard directional survey tools used on may drilling rigs. The system includes a small, rugged, programmable temperature sensor, a standard body housing, various adapters for specific applications, and a personal computer (PC) interface

  18. Planar patch-clamp force microscopy on living cells

    Energy Technology Data Exchange (ETDEWEB)

    Pamir, Evren [Center for Nano Science, Ludwig-Maximilians University, Amalienstr 54, 80799 Munich (Germany); George, Michael; Fertig, Niels [Nanion Technologies GmbH, Erzgiessereistr. 4, 80335 Munich (Germany); Benoit, Martin [Center for Nano Science, Ludwig-Maximilians University, Amalienstr 54, 80799 Munich (Germany)], E-mail: martin.benoit@physik.uni-muenchen.de

    2008-05-15

    Here we report a new combination of the patch-clamp technique with the atomic force microscope (AFM). A planar patch-clamp chip microstructured from borosilicate glass was used as a support for mechanical probing of living cells. The setup not only allows for immobilizing even a non-adherent cell for measurements of its mechanical properties, but also for simultaneously measuring the electrophysiological properties of a single cell. As a proof of principle experiment we measured the voltage-induced membrane movement of HEK293 and Jurkat cells in the whole-cell voltage clamp configuration. The results of these measurements are in good agreement with previous studies. By using the planar patch-clamp chip for immobilization, the AFM not only can image non-adhering cells, but also gets easily access to an electrophysiologically controlled cellular probe at low vibrational noise.

  19. Insulin Tolerance Test and Hyperinsulinemic-Euglycemic Clamp

    Science.gov (United States)

    Paschos, Georgios K.; FitzGerald, Garret A.

    2016-01-01

    The two tests are used to evaluate in vivo sensitivity to insulin in mouse. The hypoerinsulinemic-euglycemic clamp provides information about the sensitivity to insulin in liver and other metabolically relevant tissues.

  20. Usefulness of Kelly clamp crushing technique during hepatic resection

    OpenAIRE

    Kim, K. H.; Lee, S G

    2008-01-01

    Introduction. Recently, there are various kinds of parenchymal transection methods. The aim of this study is to evaluate the usefulness of the Kelly clamp crushing technique compared to ultrasonic dissector during hepatic resection. Materials and methods. Comparisons between 10 ultrasonic dissector group and 10 Kelly clamp crushing technique group were performed by using nine items (transaction time, right lobe volume, perioperative transfusion, total bilirubin (TB), aspartate aminotransferas...

  1. Horizontal Acoustic Barriers for Protection from Seismic Waves

    Directory of Open Access Journals (Sweden)

    Sergey V. Kuznetsov

    2011-01-01

    Full Text Available The basic idea of a seismic barrier is to protect an area occupied by a building or a group of buildings from seismic waves. Depending on nature of seismic waves that are most probable in a specific region, different kinds of seismic barriers can be suggested. Herein, we consider a kind of a seismic barrier that represents a relatively thin surface layer that prevents surface seismic waves from propagating. The ideas for these barriers are based on one Chadwick's result concerning nonpropagation condition for Rayleigh waves in a clamped half-space, and Love's theorem that describes condition of nonexistence for Love waves. The numerical simulations reveal that to be effective the length of the horizontal barriers should be comparable to the typical wavelength.

  2. Downhole geophysical observatories: best installation practices and a case history from Turkey

    Science.gov (United States)

    Prevedel, Bernhard; Bulut, Fatih; Bohnhoff, Marco; Raub, Christina; Kartal, Recai F.; Alver, Fatih; Malin, Peter E.

    2015-09-01

    Downhole sensors of different types and in various environments provide substantial benefit to signal quality. They also add the depth dimension to measurements performed at the Earths' surface. Sensor types that particularly benefit from downhole installation due to the absence of near-surface noise include piezometers, seismometers, strainmeters, thermometers, and tiltmeters. Likewise, geochemical and environmental measurements in a borehole help eliminate near-surface weathering and cultural effects. Installations from a few hundred meter deep to a few kilometer deep dramatically reduce surface noise levels—the latter noticeably also reduces the hypocentral distance for shallow microearthquakes. The laying out of a borehole network is always a compromise of local boundary conditions and the involved drilling costs. The installation depth and procedure for a long-term downhole observatory can range from time limited installations, with a retrieval option, to permanently cemented sensors. Permanently cemented sensors have proven to be long-term stable with non-deteriorating coupling and borehole integrity. However, each type needs to be carefully selected and planned according to the research aims. A convenient case study is provided by a new installation of downhole seismometers along the shoreline of the eastern Marmara Sea in Turkey. These stations are being integrated into the regional net for monitoring the North Anatolian Fault Zone. Here we discuss its design, installation, and first results. We conclude that, despite the logistical challenges and installation costs, the superior quality of downhole data puts this technique at the forefront of applied and fundamental research.

  3. Ventilation onset prior to umbilical cord clamping (physiological-based cord clamping improves systemic and cerebral oxygenation in preterm lambs.

    Directory of Open Access Journals (Sweden)

    Graeme R Polglase

    Full Text Available As measurement of arterial oxygen saturation (SpO2 is common in the delivery room, target SpO2 ranges allow clinicians to titrate oxygen therapy for preterm infants in order to achieve saturation levels similar to those seen in normal term infants in the first minutes of life. However, the influence of the onset of ventilation and the timing of cord clamping on systemic and cerebral oxygenation is not known.We investigated whether the initiation of ventilation, prior to, or after umbilical cord clamping, altered systemic and cerebral oxygenation in preterm lambs.Systemic and cerebral blood-flows, pressures and peripheral SpO2 and regional cerebral tissue oxygenation (SctO2 were measured continuously in apnoeic preterm lambs (126±1 day gestation. Positive pressure ventilation was initiated either 1 prior to umbilical cord clamping, or 2 after umbilical cord clamping. Lambs were monitored intensively prior to intervention, and for 10 minutes following umbilical cord clamping.Clamping the umbilical cord prior to ventilation resulted in a rapid decrease in SpO2 and SctO2, and an increase in arterial pressure, cerebral blood flow and cerebral oxygen extraction. Ventilation restored oxygenation and haemodynamics by 5-6 minutes. No such disturbances in peripheral or cerebral oxygenation and haemodynamics were observed when ventilation was initiated prior to cord clamping.The establishment of ventilation prior to umbilical cord clamping facilitated a smooth transition to systemic and cerebral oxygenation following birth. SpO2 nomograms may need to be re-evaluated to reflect physiological management of preterm infants in the delivery room.

  4. Seismic study of soil dynamics at Garner Valley, California

    International Nuclear Information System (INIS)

    The Garner Valley downhole array (GVDA) of force-balanced accelerometers was designed to determine the effect that near-surface soil layers have on surface ground motion by measuring in situ seismic waves at various depths. Although there are many laboratory, theoretical and numerical studies that are used to predict the effects that local site geology might have on seismic waves, there are very few direct measurements that can be used to confirm the predictions made by these methods. The effects of local site geology on seismic ground motions are critical for estimating the base motion of structures. The variations in site amplifications at particular periods can range over a factor of 20 or more in comparing amplitude spectra from rock and soil sites, e.g., Mexico City (1985) or San Francisco (1989). The basic phenomenon of nonlinear soil response, and by inference severe attenuation of seismic waves, has rarely been measured although it is commonly observed in laboratory experiments. The basic question is whether or not the local site geology amplifies are attenuates the seismic ground motion. Because the answer depends on the interaction between the local site geology and the amplitude as well as the frequency content of the incoming seismic waves, the in situ measurements must sample the depth variations of the local structure as well as record seismic waves over as wide a range as possible in amplitude and frequency

  5. An improved finite-difference calculation of downhole dynamometer cards for sucker-rod pumps

    Energy Technology Data Exchange (ETDEWEB)

    Everitt, T.A. (Chevron Oil Field Research Co. (US))

    1992-02-01

    Sucker-rod pumping is the most widely used means of artificial lift. About 85% to 90% of all producing wells in the U.S. are rod-pumped. Thus, a reliable method of analyzing these pumping system is a necessity. For many years, the surface dynamometer has been used to analyze sucker-rod systems. Interpretation of actual pump conditions from surface dynamometer cards is often difficult, if not impossible. Results obtained from surface cards are strictly qualitative and are dependent on the analyzer's expertise. The ideal analysis procedure would be to measure the actual pump conditions with a downhole dynamometer. However, this situation is not economically feasible. Therefore, an accurate method of calculating downhole pump cards form measured surface cards is needed. This paper presents a method for calculating these downhole cards that uses a finite-difference representation of the wave equation. First, a brief description of previous calculation techniques is given.

  6. Development of a High-Pressure/High-Temperature Downhole Turbine Generator

    Energy Technology Data Exchange (ETDEWEB)

    Timothy F. Price

    2007-02-01

    The objective of this project as originally outlined has been to achieve a viable downhole direct current (DC) power source for extreme high pressure, high temperature (HPHT) environments of >25,000 psi and >250 C. The Phase I investigation posed and answered specific questions about the power requirements, mode of delivery and form factor the industry would like to see for downhole turbine generator tool for the HPHT environment, and noted specific components, materials and design features of that commercial system that will require upgrading to meet the HPHT project goals. During the course of Phase I investigation the scope of the project was HPHT downhole DC power. Phase I also investigated the viability of modifying a commercial expanded, without additional cost expected to the project, to include the addition of HT batteries to the power supply platform.

  7. Numerical simulation of surface and downhole deformation induced by hydraulic fracturing

    Institute of Scientific and Technical Information of China (English)

    He Yi-Yuan; Zhang Bao-Ping; Duan Yu-Ting; Xue Cheng-Jin; Yan Xin; He Chuan; Hu Tian-Yue

    2014-01-01

    Tiltmeter mapping technology infers hydraulic fracture geometry by measuring fracture-induced rock deformation, which recorded by highly sensitive tiltmeters placed at the surface and in nearby observation wells. By referencing Okada’s linear elastic theory and Green’s function method, we simulate and analyze the surface and downhole deformation caused by hydraulic fracturing using the homogeneous elastic half-space model and layered elastic model. Simulation results suggest that there is not much difference in the surface deformation patterns between the two models, but there is a significant difference in the downhole deformation patterns when hydraulic fracturing penetrates a stratum. In such cases, it is not suitable to assume uniform elastic half-space when calculating the downhole deformation. This work may improve the accuracy and reliability of the inversion results of tiltmeter monitoring data.

  8. Development of a High Pressure/High Temperature Down-hole Turbine Generator

    Energy Technology Data Exchange (ETDEWEB)

    Ben Plamp

    2008-06-30

    As oil & natural gas deposits become more difficult to obtain by conventional means, wells must extend to deeper more heat-intensive environments. The technology of the drilling equipment required to reach these depths has exceeded the availability of electrical power sources needed to operate these tools. Historically, logging while drilling (LWD) and measure while drilling (MWD) devices utilized a wireline to supply power and communication from the operator to the tool. Lithium ion batteries were used in scenarios where a wireline was not an option, as it complicated operations. In current downhole applications, lithium ion battery (LIB) packs are the primary source for electrical power. LIB technology has been proven to supply reliable downhole power at temperatures up to 175 °C. Many of the deeper well s reach ambient temperatures above 200 °C, creating an environment too harsh for current LIB technology. Other downfalls of LIB technology are cost, limitations on charge cycles, disposal issues and possible safety hazards including explosions and fires. Downhole power generation can also be achieved by utilizing drilling fluid flow and converting it to rotational motion. This rotational motion can be harnessed to spin magnets around a series of windings to produce power proportional to the rpm experienced by the driven assembly. These generators are, in most instances, driven by turbine blades or moyno-based drilling fluid pumps. To date, no commercially available downhole power generators are capable of operating at ambient temperatures of 250 °C. A downhole power g enerator capable of operation in a 250 °C and 20,000 psi ambient environment will be an absolute necessity in the future. Dexter Magnetic Technologies’ High-Pressure High-Temperature (HPHT) Downhole Turbine Generator is capable of operating at 250 °C and 20, 000 psi, but has not been tested in an actual drilling application. The technology exists, but to date no company has been willing to

  9. Fibre optic pressure sensor for downhole monitoring in the oil industry

    OpenAIRE

    Clowes, John Redvers

    2000-01-01

    Cost-effective oil and gas production is becoming more important than ever. The availability of downhole information is seen to be the key to increasing oil-recovery efficiency, currently estimated to be 35% on average for North Sea oil-wells. Fibre-optic sensors have the potential to measure many downhole parameters. However, high-temperature, high-pressure (HTHP) fluids are shown to have adverse effects on fibre pressure-sensors and cables. All unprotected, silica-fibre, pressure sensors...

  10. A NOVEL SINGLE IMPEDANCE NETWORK BASED NEUTRAL POINT CLAMPED SEVEN LEVEL THREE PHASE INVETER WITH REDUCED CLAMPING DIODES FOR REGENERATIVE APPLICATIONS

    OpenAIRE

    T. A. Raghavendiran; C.L.Kuppuswamy

    2012-01-01

    A Multilevel Inverter (MLI) can eliminate the need for the step-up transformer and reduce the harmonics produced by the inverter. This paper presents Neutral Point Clamped (NPC) seven level inverter with less number of clamping diodes which is suitable for regenerative loads such as three phase induction motor with regenerative braking. To reduce the stress across the main switches, diodes are clamped anti-parallel to the main switches. In Earlier configurations, the numbers of clamping diode...

  11. Borehole Summary Report for Waste Treatment Plant Seismic Borehole C4996

    Energy Technology Data Exchange (ETDEWEB)

    Adams , S. C.; Ahlquist, Stephen T.; Fetters, Jeffree R.; Garcia, Ben; Rust, Colleen F.

    2007-01-28

    This report presents the field-generated borehole log, lithologic summary, and the record of samples collected during the recent drilling and sampling of the basalt interval of borehole C4996 at the Waste Treatment Plant (WTP) on the Hanford Site. Borehole C4996 was one of four exploratory borings, one core hole and three boreholes, drilled to investigate and acquire detailed stratigraphic and down-hole seismic data. This data will be used to define potential seismic impacts and refine design specifications for the Hanford Site WTP.

  12. [Pedicular clamping in major hepatectomies: clamping "of principle" or "of necessity"? A comparative study].

    Science.gov (United States)

    Le Treut, Y P; Christophe, M; Banti, J C; Berthet, B; Bricot, R

    1995-02-01

    Fifty-two consecutive patients undergoing major hepatic resection for liver tumor were divided into two groups according to the operative procedure. Group A consisted of 34 patients in whom vascular inflow occlusion was performed "de principle" during parenchymal division and intrahepatic approach of the portal structures; the mean duration of the portal triad clamping was 43 mn (ranged 17 to 70 mn). Group B patients (18 cases) had hilar division of the structures of that portion of the liver due to be removed, prior to parenchymal division was performed without vascular arrest, except in five "de necessitate" cases during 5 to 22 mn. Groups A and B were comparable in terms of patient age or status, of king of liver tumors and extent of resection. Mean operating duration (215 vs 263 mn), volume of intraoperative blood transfusion (557 vs 1019 ml), intensive care (2.5 vs 4.2 days) and total hospital stays (19.6 vs 30.5 days) were significantly reduced in group A. A higher but transient increase of amino-transferase level was the only biochemical consequence of liver ischemia in group A, whereas postoperative disturbance in serum bilirubin, prothrombin time, fibrinogen, and total protein were significantly greater in group B, probably because of the greater volume of blood transfusion in this group. Thus, routine vascular inflow occlusion with transhepatic approach of the portal structures may be an effective and innocuous procedure for major liver resection. PMID:7751341

  13. Improved DNA clamps by stacking to adjacent nucleobases

    DEFF Research Database (Denmark)

    Fatthalla, M.I.; Pedersen, Erik Bjerregaard

    2012-01-01

    Three or four aromatic rings interconnected by acetylene bridges form a stiff conjugated system with sufficient conformational freedom to make it useful to link together the two strands of a DNA clamp. Upon targeting a ssDNA, the conformational flexibility allows better stacking of the linker to...... the underlying non-planar base triplet in the formed triplex. This type of triplexes has a substantially higher thermal melting temperature which can be further improved by inserting locked nucleic acids (LNAs) in the Hoogsteen part of the clamp. An extremely high sensitivity to mismatches is observed...

  14. Plasma temperature clamping in filamentation laser induced breakdown spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Harilal, Sivanandan S.; Yeak, J.; Phillips, Mark C.

    2015-10-19

    Ultrafast laser filament induced breakdown spectroscopy is a very promising method for remote material detection. We present characteristics of plasmas generated in a metal target by laser filaments in air. Our measurements show that the temperature of the ablation plasma is clamped along the filamentation channel due to intensity clamping in a filament. Nevertheless, significant changes in radiation intensity are noticeable, and this is essentially due to variation in the number density of emitting atoms. The present results also partly explains the reason for the occurrence of atomic plume during fs LIBS in air compared to long-pulse ns LIBS.

  15. New active load voltage clamp for HF-link converters

    Energy Technology Data Exchange (ETDEWEB)

    Ljusev, P.; Andersen, M.A.E.

    2005-07-01

    This paper proposes a new active clamp for HF-link converters, which features very high efficiency by returning the clamped energy back to the primary side through a small auxiliary converter. It also increases the reliability of HF-link converters by providing an alternative load current path during malfunctions of the secondary bidirectional bridge. The feasibility of the approach is shown on audio power amplifier prototype. New integrated magnetics design is presented that incorporates both the main power and auxiliary transformer on the same magnetic core. (au)

  16. The problem of isotropic rectangular plate with four clamped edges

    Indian Academy of Sciences (India)

    C Erdem İmrak; Ismail Gerdemeli

    2007-06-01

    The examination of the exact solution of the governing equation of the rectangular plate is important for many reasons. This report discusses in exact solution of the governing equation of an isotropic rectangular plate with four clamped edges. A numerical method for clamped isotropic rectangular plate under distributed loads and an exact solution of the governing equation in terms of trigonometric and hyperbolic function are given. Finally, an illustrative example is given and the results are compared with those reported earlier. This method is found to be easier and effective. The results show reasonable agreement with other available results, but with a simpler and practical approach.

  17. Retrieving Drill Bit Seismic Signals Using Surface Seismometers

    Institute of Scientific and Technical Information of China (English)

    Linfei Wang; Huaishan Liu; Siyou Tong; Yanxin Yin; Lei Xing; Zhihui Zou; Xiugang Xu

    2015-01-01

    Seismic while drilling (SWD) is an emerging borehole seismic imaging technique that uses the downhole drill-bit vibrations as seismic source. Without interrupting drilling, SWD technique can make near-real-time images of the rock formations ahead of the bit and optimize drilling operation, with reduction of costs and the risk of drilling. However, the signal to noise ratio (SNR) of surface SWD-data is severely low for the surface acquisition of SWD data. Here, we propose a new method to retrieve the drill-bit signal from the surface data recorded by an array of broadband seismometers. Taking advantages of wavefield analysis, different types of noises are identified and removed from the surface SWD-data, resulting in the significant improvement of SNR. We also optimally synthesize seis-mic response of the bit source, using a statistical cross-coherence analysis to further improve the SNR and retrieve both the drill-bit direct arrivals and reflections which are then used to establish a reverse vertical seismic profile (RVSP) data set for the continuous drilling depth. The subsurface images de-rived from these data compare well with the corresponding images of the three-dimension surface seis-mic survey cross the well.

  18. Seismic Creep

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Seismic creep is the constant or periodic movement on a fault as contrasted with the sudden erupture associated with an earthquake. It is a usually slow deformation...

  19. Topology Optimization of an Actively Cooled Electronics Section for Downhole Tools

    DEFF Research Database (Denmark)

    Soprani, Stefano; Klaas Haertel, Jan Hendrik; Lazarov, Boyan Stefanov;

    2015-01-01

    downhole electronics unit proved to be challenging, because of the space constraints and the proximity of the cooling zone (electronics) to the heat sink (well fluid). The topology optimization approach was therefore chosen to optimize the thermal design of the actively cooled electronics section and the...

  20. Improved laser ablation U-Pb zircon geochronology through robust downhole fractionation correction

    DEFF Research Database (Denmark)

    Paton, Chad

    2010-01-01

    Elemental fractionation effects during analysis are the most significant impediment to obtaining precise and accurate U-Pb ages by laser ablation ICPMS. Several methods have been proposed to minimize the degree of downhole fractionation, typically by rastering or limiting acquisition to relativel...

  1. Early Detection and Localization of Downhole Incidents in Managed Pressure Drilling

    DEFF Research Database (Denmark)

    Willersrud, Anders; Imsland, Lars; Blanke, Mogens;

    2015-01-01

    successfully been tested on experimental data from a medium-scale horizontal flow loop in Sta- vanger, Norway. The flow loop represents a 700 m borehole with emulation of the following downhole contingencies: drillstring washout, drill bit nozzle plugging, gas influx and fluid loss. In the tests...

  2. Fault diagnosis of downhole drilling incidents using adaptive observers and statistical change detection

    DEFF Research Database (Denmark)

    Willersrud, Anders; Blanke, Mogens; Imsland, Lars;

    2015-01-01

    Downhole abnormal incidents during oil and gas drilling causes costly delays, any may also potentially lead to dangerous scenarios. Dierent incidents willcause changes to dierent parts of the physics of the process. Estimating thechanges in physical parameters, and correlating these with changes ...

  3. COMPARISON OF VIBRATION PERFORMANCE OF A SANDWICH CLAMPED-CLAMPED BEAM WITH MAGNETS VERSUS CONVENTIONAL CONSTRAINED DAMPING TREATMENTS

    Institute of Scientific and Technical Information of China (English)

    ZhengHuiming; HeZeng; LiMing; ZhaoGaoyu

    2004-01-01

    This paper investigates how the magnetic constrained layer damping (MCLD) treatment suppresses the displacement and acceleration resonant peak of a clamped-clamped beam. Because MCLI) treatment reduces input work and gives more dissipation energy, the vibration from external excitation can be effectively suppressed.The vibration reduction effects of MCLD and other conventional constrained damping treatments are also evaluated. In many cases, using MCLD treatments can yield smaller displacement and acceleration resonant peak especially in mode 1 compared to the other treatments without greatly changing the natural frequencies of the base beam.

  4. Seismic reflections associated with submarine gas hydrates

    Energy Technology Data Exchange (ETDEWEB)

    Andreassen, K.

    1995-12-31

    Gas hydrates are often suggested as a future energy resource. This doctoral thesis improves the understanding of the concentration and distribution of natural submarine gas hydrates. The presence of these hydrates are commonly inferred from strong bottom simulating reflection (BSR). To investigate the nature of BSR, this work uses seismic studies of hydrate-related BSRs at two different locations, one where gas hydrates are accepted to exist and interpreted to be very extensive (in the Beaufort Sea), the other with good velocity data and downhole logs available (offshore Oregon). To ascertain the presence of free gas under the BSR, prestack offset data must supplement near-vertical incidence seismic data. A tentative model for physical properties of sediments partially saturated with gas hydrate and free gas is presented. This model, together with drilling information and seismic data containing the BSR beneath the Oregon margin and the Beaufort Sea, made it possible to better understand when to apply the amplitude-versus-offset (AVO) method to constrain BSR gas hydrate and gas models. Distribution of natural gas hydrates offshore Norway and Svalbard is discussed and interpreted as reflections from the base of gas hydrate-bearing sediments, overlying sediments containing free gas. Gas hydrates inferred to exist at the Norwegian-Svalbard continental margin correlate well with Cenozoic depocenters, and the associated gas is assumed to be mainly biogenic. Parts of that margin have a high potential for natural gas hydrates of both biogenic and thermogenic origin. 235 refs., 86 figs., 4 tabs.

  5. Mechanical stability of multidomain proteins and novel mechanical clamps.

    Science.gov (United States)

    Sikora, Mateusz; Cieplak, Marek

    2011-06-01

    We estimate the size of mechanostability for 318 multidomain proteins which are single-chain and contain up to 1021 amino acids. We predict existence of novel types of mechanical clamps in which interdomain contacts play an essential role. Mechanical clamps are structural regions which are the primary source of a protein's resistance to pulling. Among these clamps there is one that opposes tensile stress due to two domains swinging apart. This movement strains and then ruptures the contacts that hold the two domains together. Another clamp also involves tensile stress but it originates from an immobilization of a structural region by a surrounding knot-loop (without involving any disulfide bonds). Still another mechanism involves shear between helical regions belonging to two domains. We also consider the amyloid-prone cystatin C which provides an example of a two-chain 3D domain-swapped protein. We predict that this protein should withstand remarkably large stress, perhaps of order 800 pN, when inducing a shearing strain. The survey is generated through molecular dynamics simulations performed within a structure-based coarse grained model. PMID:21465555

  6. Hyperinsulinemic Euglycemic Clamp for cardiac PET: An Initial Experience

    International Nuclear Information System (INIS)

    Full text: Aim: To study the adequacy of hyperinsulinemic euglycemic clamp to yield technically adequate and clinically meaningful cardiac scans in diabetic patients. 18 F- FDG scans are of suboptimal quality as high level of glucose competes with flurodeoxyglucose for cardiac uptake. Thus to allow quick stabilization of metabolic environment and to get superior quality images, insulin clamp is an accepted technique. Methods: 8 patients (7 males and 1 female) of ages ranging from 43 to 69 years were evaluated in this ongoing study, referred for estimation of myocardial viability post myocardial infarction. All patients were required 6 hours fasting. Initially they were injected with 20 milicurie of Technetium Tetrofosmin and myocardial perfusion imaging done 1 hour later on GE dual head gamma camera. Blood glucose levels were checked. Their blood sugars were in the range of 130mg% - 170mg%. Insulin clamp was applied depending upon the glucose level obtained, according to ASNC guidelines for PET Myocardial Glucose Metabolism and Perfusion Imaging. On stabilization of blood glucose levels, 10 milicurie of 18 F- FDG was injected. Imaging was done 1 hour later on Dedicated 16 slice STE PET CT scanner. There was no incidence of hypoglycemia or any other side effects during the procedure. Entire procedure including tetrofosmin imaging took approximately 4 hours. Results: Good quality images were obtained after the use of insulin clamp. Conclusion: Infusion of insulin and glucose gives stable plasma glucose and serum insulin levels during imaging. The insulin clamp technique makes it possible to adjust and maintain a metabolic steady state during PET study. It does not alter 18F-FDG uptake patterns in different myocardial areas. It permits the use of smaller FDG doses for the patient and gives superior quality images. The technique is safe and should improve both the clinical use and cost effectiveness of FDG-PET imaging for identification of injured but viable myocardium

  7. Seismic VSP Investigations at Olkiluoto, 2005

    International Nuclear Information System (INIS)

    Posiva Oy carries out R and D related tasks for spent nuclear fuel disposal in Finland. The site characterization has been conducted since 1987 in Olkiluoto in western Finland. The ONKALO underground characterization facility has been under construction since 2004. Vibrometric Oy has been contracted to carry out seismic VSP survey in four drillholes in the immediate vicinity of ONKALO, for the characterization of the seismically responsive structures. Four drillholes, KR8, KR27, KR29 and KR38 were included to the project. Seven seismic source locations on ground surface were used for each drillhole. The source locations were optimized with respect to the drillhole and ONKALO and were configured as linear arrays to produce optimum imaging focused on the ONKALO volume. A mechanical Vibsist source, using a hydraulic rock breaker mounted on a 22 t excavator, was used as source of seismic signal. The signal was recorded with downhole 3-component geophones. The recording array was 8-level long, with 5 m spacing between levels. Acquisition was run throughout the drillholes. Processing of the VSP profiles consisted of time decoding of the impact sequences, filtering and image point (IP) transform. The interpretation was carried out interactively, seeking for best match of orientation of each reflection according to different borehole profiles where the features were seen. The interpretations were built as an add-on to a previous seismic model of the site. The most distinct reflectors were interpreted, compiled to as a part of a terrain model composed of 3D surfaces, and transferred digitally together with other results (3D elements of reflector locations) into Posiva's 3D modeling system. Some of the reflectors have already received direct confirmation from ONKALO observations. (orig.)

  8. Strong seismic ground motion propagation

    International Nuclear Information System (INIS)

    At the McGee Creek, California, site, 3-component strong-motion accelerometers are located at depths of 166 m, 35 m and 0 m. The surface material is glacial moraine, to a depth of 30.5 m, overlying homfels. Accelerations were recorded from two California earthquakes: Round Valley, ML 5.8, November 23, 1984, 18:08 UTC and Chalfant Valley, ML 6.4, July 21, 1986, 14:42 UTC. By separating out the SH components of acceleration, we were able to determine the orientations of the downhole instruments. By separating out the SV component of acceleration, we were able to determine the approximate angle of incidence of the signal at 166 m. A constant phase velocity Haskell-Thomson model was applied to generate synthetic SH seismograms at the surface using the accelerations recorded at 166 m. In the frequency band 0.0 - 10.0 Hz, we compared the filtered synthetic records to the filtered surface data. The onset of the SH pulse is clearly seen, as are the reflections from the interface at 30.5 m. The synthetic record closely matches the data in amplitude and phase. The fit between the synthetic accelerogram and the data shows that the seismic amplification at the surface is a result of the contrast of the impedances (shear stiffnesses) of the near surface materials

  9. Vibration control of a flexible clamped-clamped plate based on an improved FULMS algorithm and laser displacement measurement

    Science.gov (United States)

    Xie, Lingbo; Qiu, Zhi-cheng; Zhang, Xian-min

    2016-06-01

    This paper presents a novel active resonant vibration control experiment of a flexible clamped-clamped plate using an improved filtered-U least mean square (FULMS) algorithm and laser displacement measurement. Different from the widely used PZT sensors or acceleration transducers, the vibration of the flexible clamped-clamped plate is measured by a non-contact laser displacement measurement sensor with higher measurement accuracy and without additional load to the plate. The conventional FULMS algorithm often uses fixed step size and needs reference signal related to the external disturbance signal. However, the fixed step size method cannot obtain a fast convergence speed and it will result in a low residual error. Thus, a variable step size method is investigated. In addition, it is difficult to extract reference signal related to the vibration source directly in the practical application. Therefore, it is practically useful that a reference signal is constructed by both the controller parameters and the vibration residual signal. The experimental results demonstrate that the improved FULMS algorithm has better vibration control effect than the proportional derivative (PD) feedback control algorithm and the fixed step-size control algorithm.

  10. New Developments in Long-Term Downhole Monitoring Arrays

    Directory of Open Access Journals (Sweden)

    Jochem Kück

    2006-03-01

    Full Text Available The long-term observation of active geological processes is a major research goal in an increasing number of scientific drilling projects. An extended monitoring phase within a potentially hostile environment (e.g., temperature, pressure, salinity requires new long-lasting and robust instrumentation currently unavailable from either industry or academia. Extended exposure of instrument packages to extreme conditions will typically cause seals to weaken and fail,electronic parts to break under permanent load, and sensors to degrade or develop strong drift. In the framework of scientific exploration, there are currently several major research projects targeting fault zone drilling and in situ measurements to monitor physical and chemical conditions before, during, and after seismic events. Planning has now begun for tool development, testing, and continuous long-term monitoring for the San Andreas Fault Zone Observatory at Depth, SAFOD (Parkfi eld, Calif., U.S.A.; See article on page 32..

  11. Seismic instrumentation

    International Nuclear Information System (INIS)

    For better understanding of the specification for seismic instrumentation of a nuclear power plant, the lecture gives some fundamental remarks to the seismic risk in the Federal Republic of Germany and to the data characterizing an earthquake event. Coming from the geophysical properties of an earthquake, the quantities are explained which are used in the design process of nuclear power plants. This process is shortly described in order to find the requirements for the specification of the seismic instrumentation. In addition the demands of licensing authorities are given. As an example the seismic instrumentation of KKP-1, BWR, is shown. The paper deals with kind and number of instruments, their location in the plant and their sensitivity and calibration. Final considerations deal with the evaluation of measured data and with plant operation after an earthquake. Some experience concerning the earthquake behaviour of equipment not designed to withstand earthquake loads is mentioned. This experience has initiated studies directed to quantification of the degree of conservatism of the assumptions in the seismic design of nuclear power plants. A final garget of these studies are more realistic design rules. (RW)

  12. Four- year thyroidectomy procedures: Ligasure Vs. clamp & tie

    Directory of Open Access Journals (Sweden)

    Pazoki AR

    2010-01-01

    Full Text Available "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: The electrothermal bipolar vessel sealing system is an adjunct to the hemostatic technique, which has been successfully used in many abdominal operation, recently made available to thyroid surgery. The purpose of this was compare the outcomes of new hemostatic technique with traditional method of clamp and tie."n"nMethods: In this retrospective cross-sectional study has been done in surgery's wards of Milad & Rasul- Akram hospitals in Tehran, Iran. During three- year period between 2005-2008 all patients with different kind of pathology that need operation were assessed. Time duration of surgery and hospital stay and post surgery complication in two group, ligasure (n=45 and clamp & tie (n=145 were compared with each others."n"nResults: No difference was seen between two groups in time duration of surgery. Significant difference was seen in hospital stay and complications of surgery. Time of operation in Ligasure group was 132/22 minute and in clamp and tie group was 130/10 minute. There was found significant difference in duration of hospital stay and postoperative complication. Hypocalcemia were seen in two and 24 cases in Ligasure and Clamp & Tie group, respectively (p<0.05. Hospital stay was 2

  13. Seismic Studies

    Energy Technology Data Exchange (ETDEWEB)

    R. Quittmeyer

    2006-09-25

    This technical work plan (TWP) describes the efforts to develop and confirm seismic ground motion inputs used for preclosure design and probabilistic safety 'analyses and to assess the postclosure performance of a repository at Yucca Mountain, Nevada. As part of the effort to develop seismic inputs, the TWP covers testing and analyses that provide the technical basis for inputs to the seismic ground-motion site-response model. The TWP also addresses preparation of a seismic methodology report for submission to the U.S. Nuclear Regulatory Commission (NRC). The activities discussed in this TWP are planned for fiscal years (FY) 2006 through 2008. Some of the work enhances the technical basis for previously developed seismic inputs and reduces uncertainties and conservatism used in previous analyses and modeling. These activities support the defense of a license application. Other activities provide new results that will support development of the preclosure, safety case; these results directly support and will be included in the license application. Table 1 indicates which activities support the license application and which support licensing defense. The activities are listed in Section 1.2; the methods and approaches used to implement them are discussed in more detail in Section 2.2. Technical and performance objectives of this work scope are: (1) For annual ground motion exceedance probabilities appropriate for preclosure design analyses, provide site-specific seismic design acceleration response spectra for a range of damping values; strain-compatible soil properties; peak motions, strains, and curvatures as a function of depth; and time histories (acceleration, velocity, and displacement). Provide seismic design inputs for the waste emplacement level and for surface sites. Results should be consistent with the probabilistic seismic hazard analysis (PSHA) for Yucca Mountain and reflect, as appropriate, available knowledge on the limits to extreme ground

  14. Seismic Studies

    International Nuclear Information System (INIS)

    This technical work plan (TWP) describes the efforts to develop and confirm seismic ground motion inputs used for preclosure design and probabilistic safety 'analyses and to assess the postclosure performance of a repository at Yucca Mountain, Nevada. As part of the effort to develop seismic inputs, the TWP covers testing and analyses that provide the technical basis for inputs to the seismic ground-motion site-response model. The TWP also addresses preparation of a seismic methodology report for submission to the U.S. Nuclear Regulatory Commission (NRC). The activities discussed in this TWP are planned for fiscal years (FY) 2006 through 2008. Some of the work enhances the technical basis for previously developed seismic inputs and reduces uncertainties and conservatism used in previous analyses and modeling. These activities support the defense of a license application. Other activities provide new results that will support development of the preclosure, safety case; these results directly support and will be included in the license application. Table 1 indicates which activities support the license application and which support licensing defense. The activities are listed in Section 1.2; the methods and approaches used to implement them are discussed in more detail in Section 2.2. Technical and performance objectives of this work scope are: (1) For annual ground motion exceedance probabilities appropriate for preclosure design analyses, provide site-specific seismic design acceleration response spectra for a range of damping values; strain-compatible soil properties; peak motions, strains, and curvatures as a function of depth; and time histories (acceleration, velocity, and displacement). Provide seismic design inputs for the waste emplacement level and for surface sites. Results should be consistent with the probabilistic seismic hazard analysis (PSHA) for Yucca Mountain and reflect, as appropriate, available knowledge on the limits to extreme ground motion at

  15. High resolution seismic reflection profiling at Aberdeen Proving Grounds, Maryland

    International Nuclear Information System (INIS)

    The effectiveness of shallow high resolution seismic reflection (i.e., resolution potential) to image geologic interfaces between about 70 and 750 ft at the Aberdeen Proving Grounds, Maryland (APG), appears to vary locally with the geometric complexity of the unconsolidated sediments that overlay crystalline bedrock. The bedrock surface (which represents the primary geologic target of this study) was imaged at each of three test areas on walkaway noise tests and CDP (common depth point) stacked data. Proven high resolution techniques were used to design and acquire data on this survey. Feasibility of the technique and minimum acquisition requirements were determined through evaluation and correlation of walkaway noise tests, CDP survey lines, and a downhole velocity check shot survey. Data processing and analysis revealed several critical attributes of shallow seismic data from APG that need careful consideration and compensation on reflection data sets. This survey determined: (1) the feasibility of the technique, (2) the resolution potential (both horizontal and vertical) of the technique, (3) the optimum source for this site, (4) the optimum acquisition geometries, (5) general processing flow, and (6) a basic idea of the acoustic variability across this site. Source testing involved an accelerated weight drop, land air gun, downhole black powder charge, sledge hammer/plate, and high frequency vibrator. Shallow seismic reflection profiles provided for a more detailed picture of the geometric complexity and variability of the distinct clay sequences (aquatards), previously inferred from drilling to be present, based on sparse drill holes and basewide conceptual models. The seismic data also reveal a clear explanation for the difficulties previously noted in correlating individual, borehole-identified sand or clay units over even short distances

  16. Review: The lord of the rings: Structure and mechanism of the sliding clamp loader.

    Science.gov (United States)

    Kelch, Brian A

    2016-08-01

    Sliding clamps are ring-shaped polymerase processivity factors that act as master regulators of cellular replication by coordinating multiple functions on DNA to ensure faithful transmission of genetic and epigenetic information. Dedicated AAA+ ATPase machines called clamp loaders actively place clamps on DNA, thereby governing clamp function by controlling when and where clamps are used. Clamp loaders are also important model systems for understanding the basic principles of AAA+ mechanism and function. After nearly 30 years of study, the ATP-dependent mechanism of opening and loading of clamps is now becoming clear. Here I review the structural and mechanistic aspects of the clamp loading process, as well as comment on questions that will be addressed by future studies. © 2016 Wiley Periodicals, Inc. Biopolymers 105: 532-546, 2016. PMID:26918303

  17. Seismic monitoring results from the first 6 months of CO2 injection at the Aquistore geological storage site, Saskatchewan, Canada

    Science.gov (United States)

    Daley, T. M.; White, D. J.; Stork, A.; Schmitt, D. R.; Worth, K.; Harris, K.; Roberts, B.; Samson, C.; Kendal, M. J.

    2015-12-01

    The Aquistore Project, located in SE Saskatchewan, Canada, is a demonstration project for CO2 storage in a deep saline aquifer. CO2 captured from a nearby coal-fired power plant is being injected into a brine-filled sandstone formation at 3100-3300 m depth. CO2 injection commenced in April, 2015, at initial rates of up to 250 tonnes per day. Seismic monitoring methods have been employed to track the subsurface CO2 plume and to record any injection-induced microseismicity. Active seismic methods utilized include 4D surface seismics using a sparse permanent array, 4D vertical seismic profiles (VSP) with both downhole geophones and a fiber optic distributed acoustic sensor (DAS) system. Pre-injection baseline seismic surveys have established very good repeatability with NRMS values as low as 0.07. 3D finite-difference seismic modelling of fluid flow simulations is used with the repeatability estimates to determine the appropriate timing for the first CO2 monitor surveys. Time-lapse logging is being conducted on a regular basis to provide in situ measurement of the change in seismic velocity associated with changes in CO2 saturation. Continuous passive seismic recording has been ongoing since the summer of 2012 to establish background local seismicity prior to the start of CO2 injection. Passive monitoring is being conducted using two, 2.5 km long, orthogonal linear arrays of surface geophones.with 3-component short-period geophones, 3 broadband surface seismometers, and an array of 3-component short-period geophones in an observation well. No significant injection-related seismicity (Mw > -1) has been detected at the surface during the first 4 months of CO2 injection. On-going analysis of the downhole passive data will provide further information as to the occurrence of lower magnitude microseismicity (Mw of -1 to -3).

  18. Downhole well log and core montages from the Mount Elbert Gas Hydrate Stratigraphic Test Well, Alaska North Slope

    Science.gov (United States)

    Collett, T.S.; Lewis, R.E.; Winters, W.J.; Lee, M.W.; Rose, K.K.; Boswell, R.M.

    2011-01-01

    The BPXA-DOE-USGS Mount Elbert Gas Hydrate Stratigraphic Test Well was an integral part of an ongoing project to determine the future energy resource potential of gas hydrates on the Alaska North Slope. As part of this effort, the Mount Elbert well included an advanced downhole geophysical logging program. Because gas hydrate is unstable at ground surface pressure and temperature conditions, a major emphasis was placed on the downhole-logging program to determine the occurrence of gas hydrates and the in-situ physical properties of the sediments. In support of this effort, well-log and core data montages have been compiled which include downhole log and core-data obtained from the gas-hydrate-bearing sedimentary section in the Mount Elbert well. Also shown are numerous reservoir parameters, including gas-hydrate saturation and sediment porosity log traces calculated from available downhole well log and core data. ?? 2010.

  19. Design of Voltage-clamp-controlled Current-clamp%电压钳控制电流钳的设计

    Institute of Scientific and Technical Information of China (English)

    熊俊; 樊枫; 瞿安连

    2008-01-01

    研究证明,传统膜片钳放大器在电流钳模式下记录到的快速电压信号会存在失真,且造成失真的根本原因是由于膜片钳的探头电路设计.为了克服这些缺陷重新设计了一种探头,新探头电路不仅能像传统的电压跟随器一样测量瞬态电压,而且适用于传统的电压钳工作模式.此外,一种命名为电压钳控制的电流钳技术被应用来改进传统的膜片钳放大器.用可变的低通滤波器来调整电压钳模块的响应速度,从而在实现膜电位钳位的同时准确记录快速电压信号.桥平衡电路用来消除命令电流流过串联电阻时带来的电压误差.而传统膜片钳中的快电容补偿环节则被改进用来补偿电极分布电容和探头放大器输入电容并提高电流钳模式下系统的响应速度.细胞模型实验结果表明,改进后的膜片钳放大器能够满足电生理研究中快速电位变化测量的需要.%Previous research rcvealed that distortion is detected in transient voltage signal recorded with traditional patch clamp amplifier under current clamp mode, which is essentially resulted by electronic design of the headstage of the patch clamp. A new kind of headstage is designed to modify the defect, the circuit of which not only measures the transient potentials as the classical voltage follower does but also is quite suitable for the standard voltage-clamp mode. Furthermore, the technique of voltage-clamp-controlled current-clamp is applied for modifying the conventional patch-clamp amplifier, the variable low-pass filter is added into the circuit to reduce the response speed of voltage-clamp module, thus the transient potentials changes can be measured while membrane potential is kept at a constant value. Bridge balance circuitry is designed to eliminate the voltage drop while the variable current injected into the electrode. And fast capacitance compensation stage of conventional PCA is modified to nentralize the

  20. Gigaseal formation in patch clamping with applications of nanotechnology

    CERN Document Server

    Malboubi, Majid

    2014-01-01

    This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of g...

  1. A new configuration for multilevel converters with diode clamped topology

    DEFF Research Database (Denmark)

    Nami, A.; Zare, F.; Ledwich, G.; Ghosh, A.; Blaabjerg, Frede

    points in this type of converter is dc-voltage control. In this paper, a novel multi output dc-dc converter connected to a diode clamped topology is presented. This converter, for a given duty cycles, is able to regulate the capacitor voltage to provide an appropriate input voltage for NPC regardless of......Due to the increased use of renewable energy and power elctronic applications, more multilevel converters (MLC) are developed. A Neutral Point Clamped (NPC) inverter is one of the most used multilevel topologies for wind turbine (WT) and photovoltaic (PV) applications. One of the most crucial...... load changes which can avoid neutral point balancing problem in such converters. In addition, the presented topology is suitable for renewable energy systems to boost the low rectified output-voltage. In order to verify the proposed topology, steady state analysis, modelling and simulations are carried...

  2. The early effects of delayed cord clamping in term infants born to Libyan mothers

    NARCIS (Netherlands)

    Emhamed, Musbah Omar; van Rheenen, Patrick; Brabin, Bernard J

    2004-01-01

    This study was conducted to evaluate the haematological effects of the timing of umbilical cord clamping in term infants 24 h after birth in Libya. Mother-infant pairs were randomly assigned to early cord clamping (within 10s after delivery) or delayed clamping (after the cord stopped pulsating). Ma

  3. Gamma radiation methods for clamp-on multiphase flow metering

    OpenAIRE

    Blaney, S

    2008-01-01

    The development of a cost-effective multiphase flow meter to determine the individual phase flow rates of oil, water and gas was investigated through the exploitation of a single clamp-on gamma densitometer and signal processing techniques. A fast-sampling (250 Hz) gamma densitometer was installed at the top of the 10.5 m high, 108.2 mm internal diameter, stainless steel catenary riser in the Cranfield University multiphase flow test facility. Gamma radiation attenuation data w...

  4. Analysis of Control Strategies for Diode Clamped Multilevel Inverter

    OpenAIRE

    C. R. Balamurugan; S. P. NATARAJAN; R. Revathy

    2013-01-01

    This paper presents the comparison of various Pulse Width Modulation (PWM) strategies for the three phase Diode Clamped Multi Level Inverter (DCMLI). The main contribution of this paper is the proposal of new modulation schemes with Variable Amplitude (VA) and various new schemes adopting the constant switching frequency and also variable switching frequency multicarrier control freedom degree combination concepts are developed and simulated for the chosen three phase DCMLI. The three phase D...

  5. Stretching of proteins in a force-clamp

    OpenAIRE

    Szymczak, Piotr; Cieplak, Marek

    2005-01-01

    Mechanically induced protein unfolding in the force-clamp apparatus is shown, in a coarse-grained model of ubiquitin, to have lognormal statistics above a treshold force and exponential below it. Correspondingly, the mean unfolding time is slowly varying and exponentially decreases as a function of the force. The time dependencies of the end-to-end distances are also distinct. The time sequence of unfolding events weakly depends on force and much of it resembles that for stretching at constan...

  6. The human dynamic clamp as a paradigm for social interaction

    OpenAIRE

    Dumas, Guillaume; de Guzman, Gonzalo C.; Tognoli, Emmanuelle; Kelso, J. A. Scott

    2014-01-01

    The human dynamic clamp (HDC) is proposed as a general paradigm for studies of elementary forms of social behavior in complex biological systems. HDC enables parametric control of real-time bidirectional interaction between humans and empirically grounded theoretical models of coordination dynamics. It thus provides necessary experimental access for laboratory investigations, while preserving the reciprocity and open boundary conditions inherent in daily life social interactions. As proof of ...

  7. POLYCYTHEMIA RISK IN NEONATES WITH DELAYED CORD CLAMPING

    Directory of Open Access Journals (Sweden)

    I Gusti Agung Ayu Sri Wulandari Pramana

    2013-07-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE Delayed Cord Clamping is still a controversial issue in the world of medicine. Until recently, the perfect times to do a Delayed Cord Clamping in various parts of the Earth in the world are still very diverse. There are a few things that became a major concern associated with Delayed Cord Clamping, one of which is the increase in blood volume due to an increase in the number of erythrocytes which led to occurrence of neonatal polycythemia which later can lead to various complications. Polycythemia in neonates is a situation where the value of venous blood hematokrit over 65% (0.65 or the amount of hemoglobin more than 22gr/Dl. However, to date the data from a variety of the latest research and analysis of the studies mention that the polycythemia that occurs in neonates with umbilical cord pinching the flush delay was mild polycythemia that can improve after 48 to 72 hours after the baby is born.

  8. Seismic Tomography.

    Science.gov (United States)

    Anderson, Don L.; Dziewonski, Adam M.

    1984-01-01

    Describes how seismic tomography is used to analyze the waves produced by earthquakes. The information obtained from the procedure can then be used to map the earth's mantle in three dimensions. The resulting maps are then studied to determine such information as the convective flow that propels the crustal plates. (JN)

  9. Seismic Symphonies

    Science.gov (United States)

    Strinna, Elisa; Ferrari, Graziano

    2015-04-01

    The project started in 2008 as a sound installation, a collaboration between an artist, a barrel organ builder and a seismologist. The work differs from other attempts of sound transposition of seismic records. In this case seismic frequencies are not converted automatically into the "sound of the earthquake." However, it has been studied a musical translation system that, based on the organ tonal scale, generates a totally unexpected sequence of sounds which is intended to evoke the emotions aroused by the earthquake. The symphonies proposed in the project have somewhat peculiar origins: they in fact come to life from the translation of graphic tracks into a sound track. The graphic tracks in question are made up by copies of seismograms recorded during some earthquakes that have taken place around the world. Seismograms are translated into music by a sculpture-instrument, half a seismograph and half a barrel organ. The organ plays through holes practiced on paper. Adapting the documents to the instrument score, holes have been drilled on the waves' peaks. The organ covers about three tonal scales, starting from heavy and deep sounds it reaches up to high and jarring notes. The translation of the seismic records is based on a criterion that does match the highest sounds to larger amplitudes with lower ones to minors. Translating the seismogram in the organ score, the larger the amplitude of recorded waves, the more the seismogram covers the full tonal scale played by the barrel organ and the notes arouse an intense emotional response in the listener. Elisa Strinna's Seismic Symphonies installation becomes an unprecedented tool for emotional involvement, through which can be revived the memory of the greatest disasters of over a century of seismic history of the Earth. A bridge between art and science. Seismic Symphonies is also a symbolic inversion: the instrument of the organ is most commonly used in churches, and its sounds are derived from the heavens and

  10. Biased insert for installing data transmission components in downhole drilling pipe

    Science.gov (United States)

    Hall, David R.; Briscoe, Michael A.; Garner, Kory K.; Wilde, Tyson J.

    2007-04-10

    An apparatus for installing data transmission hardware in downhole tools includes an insert insertable into the box end or pin end of drill tool, such as a section of drill pipe. The insert typically includes a mount portion and a slide portion. A data transmission element is mounted in the slide portion of the insert. A biasing element is installed between the mount portion and the slide portion and is configured to create a bias between the slide portion and the mount portion. This biasing element is configured to compensate for varying tolerances encountered in different types of downhole tools. In selected embodiments, the biasing element is an elastomeric material, a spring, compressed gas, or a combination thereof.

  11. An improved fiber optic pressure and temperature sensor for downhole application

    International Nuclear Information System (INIS)

    We report on the fabrication of a high pressure extrinsic Fabry–Perot interferometric (EFPI) fiber optic sensor for downhole applications by using a mechanical transducer. The mechanical transducer has been used for increasing the pressure sensitivity and the possibility of installation of the sensor downhole. The pressure–temperature cross-sensitivity (PTCS) problem has been solved by replacing the reflecting fiber with a metal microwire in the EFPI sensor. In this way the PTCS coefficient of the sensor was decreased from 47.25 psi °C−1 to 7 psi °C−1. By using a new EFPI design, a temperature sensor was fabricated. Further improvement in the pressure and temperature sensor has been done by developing fabrication technique and signal processing

  12. High-Temperature Motor Windings for Downhole Pumps Used in Geothermal Energy Production

    Energy Technology Data Exchange (ETDEWEB)

    Hooker, Matthew; Hazelton, Craig; Kano, Kimi

    2010-12-31

    The development of highly reliable downhole equipment is an essential element in enabling the widespread utilization of Enhanced Geothermal Systems (EGS). The downhole equipment used in these systems will be required to operate at high voltages and temperatures on the order of 200 to 250°C (and eventually to 300˚C). These conditions exceed the practical operating ranges of currently available thermoplastic wire insulations, and thus limit the operating lifetime of tools such as Electric Submersible Pumps (ESPs). In this work, high-temperature insulations based on composite materials were developed and demonstrated. The products of this work were found to exhibit electrical resistivities and dielectric breakdown strengths that PEEK at temperatures above 250C. In addition, sub-scale motor windings were fabricated and tested to validate the performance of this technology

  13. Simulation analysis of the oil/water structure in the downhole with presence of hydrocyclone separator

    Science.gov (United States)

    Jamil, I. E.; Al-Kayiem, H. H.

    2015-12-01

    The maturing oil fields with increasing water production can pose a challenge in terms of produced water handling and disposal issues. This paper presents the modelling and simulation procedure of the two-phase flow of water/oil in a downhole using ANSYS- FLUENT 14 software. The developed procedure successfully simulated the production zone and the interaction of the two fluids in a natural environment where the reservoir pressure is the sole driving force. The results show significant difference of volume distribution in the flows with different oil content. The model can become an essential tool to assist in prediction of the behavior of oil/water mixture flow in the wellbores, and to serve in designing downhole oil/water separators.

  14. Numerical Study of Water Control with Downhole Oil-Water Separation Technology

    Directory of Open Access Journals (Sweden)

    Yin Khor Yin

    2014-07-01

    Full Text Available The maturing oil fields with increasing water production can pose a challenging produced water handling and disposal issues. This paper presents a numerical study of a motorless hydrocyclone to enhance understanding of the downhole oil-water separation. The turbulence of fluid flow is obtained using K-ε Realizable Turbulence model for complex swirl dominated flow, while the interface between hydrocarbon and water is described using the Discrete Phase model. In this approach, factors which contribute to the hydrocyclone separation instability were discussed. Discussion is then extended to the relationship of residence time with pressure difference between overflow and underflow. These pressure differences are able to relate to pressure condition for high water cut well which require downhole separation.

  15. Seismic Readings from the Deepest Borehole in the New Madrid Seismic Zone

    Energy Technology Data Exchange (ETDEWEB)

    Woolery, Edward W [KY Geological Survey, Univ of KY; Wang, Zhenming [KY Geological Survey, Univ of KY; Sturchio, Neil C [Dept of earth and Env. Sciences, Univ of Ill at Chicago

    2006-03-01

    Since the 1980s, the research associated with the UK network has been primarily strong-motion seismology of engineering interest. Currently the University of Kentucky operates a strong-motion network of nine stations in the New Madrid Seismic Zone. A unique feature of the network is the inclusions of vertical strong-motion arrays, each with one or two downhole accelerometers. The deepest borehole array is 260 m below the surfaces at station VASA in Fulton County, Kentucky. A preliminary surface seismic refraction survey was conducted at the site before drilling the hole at VSAS (Woolery and Wang, 2002). The depth to the Paleozoic bedrock at the site was estimated to be approximately 595 m, and the depth to the first very stiff layer (i.e. Porters Creek Clay) was found to be about 260 m. These depths and stratigraphic interpretation correlated well with a proprietary seismic reflection line and the Ken-Ten Oil Exploration No. 1 Sanger hole (Schwalb, 1969), as well as our experience in the area (Street et al., 1995; Woolery et al., 1999).

  16. Investigation of the Dynamics of a Clamped-Clamped Microbeam Near the Third Mode Using a Partial Electrode

    KAUST Repository

    Masri, Karim M.

    2014-08-17

    We present an investigation of the dynamics of a clamped-clamped microbeam excited electrostatically near its third mode. To maximize the response at the third mode, a partial electrode configuration is utilized. A multi-mode Galerkin method is used to develop a reduced order model (ROM) of the beam. A shooting method to find the periodic motion is utilized to generate frequency response curves. The curves show hardenining behavior and dynamic pull-in. We show that the dynamic amplitude of the partial configuration is higher than that of a full electrode configuration. These results are promising for the use of higher-order modes for mass detection and for ultra sensitive resonant sensors.

  17. Feasibility of using electrical downhole heaters in Faja heavy oil reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez, R.; Bashbush, J.L.; Rincon, A. [Society of Petroleum Engineers, Richardson, TX (United States)]|[Schlumberger, Sugar Land, TX (United States)

    2008-10-15

    Numerical models were used to examine the effect of downhole heaters in enhanced oil recovery (EOR) processes in Venezuela's Orinoco reservoir. The downhole heaters were equipped with mineral-insulated cables that allowed alternating currents to flow between 2 conductors packed in a resistive core composed of polymers and graphite. The heaters were used in conjunction with steam assisted gravity drainage (SAGD) processes and also used in horizontal wells for limited amounts of time in order to accelerate production and pressure declines. The models incorporated the petrophysical and fluid characteristics of the Ayacucho area in the Faja del Orinoco. A compositional-thermal simulator was used to describe heat and fluid flow within the reservoir. A total of 8 scenarios were used to examine the electrical heaters with horizontal and vertical wells with heaters of various capacities. Results of the study were then used in an economic analysis of capitalized and operating costs. Results of the study showed that downhole heaters are an economically feasible EOR option for both vertical and horizontal wells. Use of the heaters prior to SAGD processes accelerated production and achieved higher operational efficiencies. 5 refs., 9 tabs., 15 figs.

  18. Raytheon downhole information system. Electromagnetic borehole measurements while drilling system. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Kolker, M.; Greene, A.H.; Kasevich, R.S.; Robertson, J.C.; Grossi, M.D.

    1978-03-01

    A description is given of the Raytheon Downhole Information System (RDIS), a real time electromagnetic borehole measurements while drilling system, applicable to oil, gas, and geothermal drilling. It communicates in both directions through the earth in a single hop at a downlink data rate of 3 bps and uplink rates dependent on depth--typically 6 bits/second at 10,000 ft and 2 bits/second at 15,000 ft; electromagnetic signal transmission time of approximately .1 second. Downhole hardware for communications, sensors, and power are packaged in three 30 ft subs. Downhole hardware can be developed to permit operation in a 275/sup 0/C geothermal environment. A cost analysis is included that predicts RDIS service could be economically priced at approximately $1000/day. Commercial availability depends primarily on proof of capability by demonstration in a working drilling well. The most significant portions of needed hardware are available. A description of a geothermal drilling telemetry system is included in Appendix A.

  19. Raytheon Downhole Information System: Electromagnetic lithospheric information system for geothermal drilling. Interim report

    Energy Technology Data Exchange (ETDEWEB)

    Kolker, M.; Greene, A.H.; Robertson, J.C.; Grossi, M.D.

    1977-03-31

    The Raytheon Downhole Information System (RDIS), which is designed to reduce the drilling cost and increase the energy yield of deep geothermal wells, is described. The RDIS consists of a downhole sensor package located just above the drill bit, a telemetry link in the drill string, and a well head data management and control system. The RDIS assists the driller to speed drilling, select the best depth locations for geothermal energy exploitation, and protect against catastrophes. Cost savings for drilling optimization are estimated at 24% for a 15,000 foot well under routine conditions. Even greater savings may accrue for catastrophe prevention. The telemetry link operates at an extremely low electromagnetic carrier frequency because propagation occurs directly through the earth. The resulting bit rates, 1 to 10 Hz, are adequate, however, because drilling is characterized by very long time constants, and sensor package data is efficiently preprocessed downhole. Sensors and telemetry units must withstand temperatures in excess of 200/sup 0/C. Studies and tests suggest that a goal of 275/sup 0/C is achievable with modest improvement in available solid state circuitry and power supplies. A three phase development plan is presented to provide a first commercial system in 26 months after start of Phase I field tests.

  20. Neuro-evolutionary event detection technique for downhole microseismic surveys

    Science.gov (United States)

    Maity, Debotyam; Salehi, Iraj

    2016-01-01

    Recent years have seen a significant increase in borehole microseismic data acquisition programs associated with unconventional reservoir developments such as hydraulic fracturing programs for shale oil and gas. The data so acquired is used for hydraulic fracture monitoring and diagnostics and therefore, the quality of the data in terms of resolution and accuracy has a significant impact on its value to the industry. Borehole microseismic data acquired in such environments typically suffer from propagation effects due to the presence of thin interbedded shale layers as well as noise and interference effects. Moreover, acquisition geometry has significant impact on detectability across portions of the sensor array. Our work focuses on developing robust first arrival detection and pick selection workflow for both P and S waves specifically designed for such environments. We introduce a novel workflow for refinement of picks with immunity towards significant noise artifacts and applicability over data with very low signal-to-noise ratio provided some accurate picks have already been made. This workflow utilizes multi-step hybrid detection and classification routine which makes use of a neural network based autopicker for initial picking and an evolutionary algorithm for pick refinement. We highlight the results from an actual field case study including multiple examples demonstrating immunity towards noise and compare the effectiveness of the workflow with two contemporary autopicking routines without the application of the shared detection/refinement procedure. Finally, we use a windowed waveform cross-correlation based uncertainty estimation method for potential quality control purposes. While the workflow was developed to work with the neural network based autopicker, it can be used with any other traditional autopicker and provides significant improvements in pick detection across seismic gathers.

  1. M. tuberculosis Sliding β-Clamp Does Not Interact Directly with the NAD+ -Dependent DNA Ligase

    OpenAIRE

    Vandna Kukshal; Taran Khanam; Deepti Chopra; Nidhi Singh; Sabyasachi Sanyal; Ravishankar Ramachandran

    2012-01-01

    The sliding β-clamp, an important component of the DNA replication and repair machinery, is drawing increasing attention as a therapeutic target. We report the crystal structure of the M. tuberculosis β-clamp (Mtbβ-clamp) to 3.0 Å resolution. The protein crystallized in the space group C222(1) with cell-dimensions a = 72.7, b = 234.9 & c = 125.1 Å respectively. Mtbβ-clamp is a dimer, and exhibits head-to-tail association similar to other bacterial clamps. Each monomer folds into three domains...

  2. Analysis of dynamic properties of boring bars concerning different clamping conditions

    Science.gov (United States)

    Åkesson, Henrik; Smirnova, Tatiana; Håkansson, Lars

    2009-11-01

    Boring bars are frequently used in the manufacturing industry to turn deep cavities in workpieces and are usually associated with vibration problems. This paper focuses on the clamping properties' influence on the dynamic properties of clamped boring bars. A standard clamping housing of the variety commonly used in industry today has been used. Both a standard boring bar and a modified boring bar have been considered. Two methods have been used: Euler-Bernoulli beam modeling and experimental modal analysis. It is demonstrated that the number of clamping screws, the clamping screw diameter sizes, the screw tightening torques, the order the screws are tightened has a significant influence on a clamped boring bars eigenfrequencies and its mode shapes orientation in the cutting speed—cutting depth plane. Also, the damping of the modes is influenced. The results indicate that multi-span Euler-Bernoulli beam models with pinned boundary condition or elastic boundary condition modeling the clamping are preferable as compared to a fixed-free Euler-Bernoulli beam for modeling dynamic properties of a clamped boring bar. It is also demonstrated that a standard clamping housing clamping a boring bar with clamping screws imposes non-linear dynamic boring bar behavior.

  3. Development of a hydraulic borehole seismic source

    Energy Technology Data Exchange (ETDEWEB)

    Cutler, R.P.

    1998-04-01

    This report describes a 5 year, $10 million Sandia/Industry project to develop an advanced borehole seismic source for use in oil and gas exploration and production. The development Team included Sandia, Chevron, Amoco, Conoco, Exxon, Raytheon, Pelton, and GRI. The seismic source that was developed is a vertically oriented, axial point force, swept frequency, clamped, reaction-mass vibrator design. It was based on an early Chevron prototype, but the new tool incorporates a number of improvements which make it far superior to the original prototype. The system consists of surface control electronics, a special heavy duty fiber optic wireline and draw works, a cablehead, hydraulic motor/pump module, electronics module, clamp, and axial vibrator module. The tool has a peak output of 7,000 lbs force and a useful frequency range of 5 to 800 Hz. It can operate in fluid filled wells with 5.5-inch or larger casing to depths of 20,000 ft and operating temperatures of 170 C. The tool includes fiber optic telemetry, force and phase control, provisions to add seismic receiver arrays below the source for single well imaging, and provisions for adding other vibrator modules to the tool in the future. The project yielded four important deliverables: a complete advanced borehole seismic source system with all associated field equipment; field demonstration surveys funded by industry showing the utility of the system; industrial sources for all of the hardware; and a new service company set up by their industrial partner to provide commercial surveys.

  4. Borehole Summary Report for Waste Treatment Plant Seismic Borehole C4993

    Energy Technology Data Exchange (ETDEWEB)

    Rust, Colleen F.; Barnett, D. BRENT; Bowles, Nathan A.; Horner, Jake A.

    2007-02-28

    A core hole (C4998) and three boreholes (C4993, C4996, and C4997) were drilled to acquire stratigraphic and downhole seismic data to model potential seismic impacts and to refine design specifications and seismic criteria for the Waste Treatment Plant (WTP) under construction on the Hanford Site. Borehole C4993 was completed through the Saddle Mountains Basalt, the upper portion of the Wanapum Basalt, and associated sedimentary interbeds, to provide a continuous record of the rock penetrated by all four holes and to provide access to the subsurface for geophysical measure¬ment. Presented and compiled in this report are field-generated records for the deep mud rotary borehole C4993 at the WTP site. Material for C4993 includes borehole logs, lithologic summary, and record of rock chip samples collected during drilling through the months of August through early October. The borehole summary report also includes documentation of the mud rotary drilling, borehole logging, and sample collection.

  5. Nonlinear vibrations of fluid-filled clamped circular cylindrical shells

    Science.gov (United States)

    Karagiozis, K. N.; Amabili, M.; Païdoussis, M. P.; Misra, A. K.

    2005-12-01

    In this study, the nonlinear vibrations are investigated of circular cylindrical shells, empty or fluid-filled, clamped at both ends and subjected to a radial harmonic force excitation. Two different theoretical models are developed. In the first model, the standard form of the Donnell's nonlinear shallow-shell equations is used; in the second, the equations of motion are derived by a variational approach which permits the inclusion of constraining springs at the shell extremities and taking in-plane inertial terms into account. In both cases, the solution includes both driven and companion modes, thus allowing for a travelling wave in the circumferential direction; they also include axisymmetric modes to capture the nonlinear inward shell contraction and the correct type (softening) nonlinear behaviour observed in experiments. In the first model, the clamped beam eigenfunctions are used to describe the axial variations of the shell deformation, automatically satisfying the boundary conditions, leading to a 7 degree-of-freedom (dof) expansion for the solution. In the second model, rotational springs are used at the ends of the shell, which when large enough reproduce a clamped end; the solution involves a sine series for axial variations of the shell deformation, leading to a 54 dof expansion for the solution. In both cases the modal expansions satisfy the boundary conditions and the circumferential continuity condition exactly. The Galerkin method is used to discretize the equations of motion, and AUTO to integrate the discretized equations numerically. When the shells are fluid-filled, the fluid is assumed to be incompressible and inviscid, and the fluid structure interaction is described by linear potential flow theory. The results from the two theoretical models are compared with existing experimental data, and in all cases good qualitative and quantitative agreement is observed.

  6. Stretching of proteins in a force-clamp

    Energy Technology Data Exchange (ETDEWEB)

    Szymczak, P [Institute of Theoretical Physics, Warsaw University, ulica Hoza 69, 00-681 Warsaw (Poland); Cieplak, Marek [Institute of Physics, Polish Academy of Sciences, Aleja Lotnikow 32/46, 02-668 Warsaw (Poland)

    2006-01-11

    Mechanically induced protein unfolding in the force-clamp apparatus is shown, in a coarse-grained model of ubiquitin, to have lognormal statistics above a threshold force and exponential statistics below it. Correspondingly, the mean unfolding time is slowly varying and exponentially decreases as a function of the force. The time dependences of the end-to-end distances are also distinct. The time sequence of unfolding events weakly depends on force and much of it resembles that for stretching at constant speed. A more complicated time dependence arises for integrin. (letter to the editor)

  7. Five-level Z-source diode-clamped inverter

    DEFF Research Database (Denmark)

    Gao, F.; Loh, Poh Chiang; Blaabjerg, Frede;

    2010-01-01

    This study proposes a five-level Z-source diode-clamped inverter designed with two intermediate Z-source networks connected between the dc input sources and rear-end inverter circuitry. By partially shorting the Z-source networks, new operating states not previously reported for two-level Z...... scheme, which always inserts two partial shoot-through states per half carrier cycle for smooth balanced operation. Theoretical findings and practical issues identified are eventually verified by constructing a scaled down laboratory prototype for testing....

  8. Characterizing Micro- and Macro-Scale Seismicity from Bayou Corne, Louisiana

    Science.gov (United States)

    Baig, A. M.; Urbancic, T.; Karimi, S.

    2013-12-01

    The initiation of felt seismicity in Bayou Corne, Louisiana, coupled with other phenomena detected by residents on the nearby housing development, prompted a call to install a broadband seismic network to monitor subsurface deformation. The initial deployment was in place to characterize the deformation contemporaneous with the formation of a sinkhole located in close proximity to a salt dome. Seismic events generated during this period followed a swarm-like behaviour with moment magnitudes culminating around Mw2.5. However, the seismic data recorded during this sequence suffer from poor signal to noise, onsets that are very difficult to pick, and the presence of a significant amount of energy arriving later in the waveforms. Efforts to understand the complexity in these waveforms are ongoing, and involve invoking the complexities inherent in recording in a highly attenuating swamp overlying a complex three-dimensional structure with the strong material property contrast of the salt dome. In order to understand the event character, as well as to locally lower the completeness threshold of the sequence, a downhole array of 15 Hz sensors was deployed in a newly drilled well around the salt dome. Although the deployment lasted a little over a month in duration, over 1000 events were detected down to moment magnitude -Mw3. Waveform quality tended to be excellent, with very distinct P and S wave arrivals observable across the array for most events. The highest magnitude events were seen as well on the surface network and allowed for the opportunity to observe the complexities introduced by the site effects, while overcoming the saturation effects on the higher-frequency downhole geophones. This hybrid downhole and surface array illustrates how a full picture of subsurface deformation is only made possible by combining the high-frequency downhole instrumentation to see the microseismicity complemented with a broadband array to accurately characterize the source

  9. A NOVEL SINGLE IMPEDANCE NETWORK BASED NEUTRAL POINT CLAMPED SEVEN LEVEL THREE PHASE INVETER WITH REDUCED CLAMPING DIODES FOR REGENERATIVE APPLICATIONS

    Directory of Open Access Journals (Sweden)

    T. A. Raghavendiran

    2012-09-01

    Full Text Available A Multilevel Inverter (MLI can eliminate the need for the step-up transformer and reduce the harmonics produced by the inverter. This paper presents Neutral Point Clamped (NPC seven level inverter with less number of clamping diodes which is suitable for regenerative loads such as three phase induction motor with regenerative braking. To reduce the stress across the main switches, diodes are clamped anti-parallel to the main switches. In Earlier configurations, the numbers of clamping diodes were increased proportionately with thenumber of levels and the number of impedance network required increases with increase number of levels. Therefore the cost and converter size increases as the number of levels goes high. In this paper a single impedance network based seven level neutral point clamped inverter with reduced number of clamping diodes without deterioration in performance is proposed, the simulation of proposed circuit is carried out using MATLAB/Simulink and the results for the same are presented. The MOSFET internal capacitance and body diodes are used for active clamping which eliminates the need for snubber circuit.

  10. Performance Evaluation of Multicarrier SPWM Strategies for Three Phase Z - source Seven Level Diode Clamped Inverter

    OpenAIRE

    V.Arun#1 , B.Shanthi

    2013-01-01

    This paper presents multicarrier PWM strategies for three phase diode clamped seven level Z-source inverter. Multilevel inverters posses the advantage of reduced harmonics,high power capability and high voltage level. Impedance network in the diode clamped multilevel inverter circuit will perform boost operation. This paper focuses on multicarriersinusoidal pulse width modulation (MCSPWM) strategy for the three phase seven level Z-source diode clamped inverter. Performance parameters of three...

  11. Microchip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording.

    Science.gov (United States)

    Harrison, Reid R; Kolb, Ilya; Kodandaramaiah, Suhasa B; Chubykin, Alexander A; Yang, Aimei; Bear, Mark F; Boyden, Edward S; Forest, Craig R

    2015-02-15

    Patch clamping is a gold-standard electrophysiology technique that has the temporal resolution and signal-to-noise ratio capable of reporting single ion channel currents, as well as electrical activity of excitable single cells. Despite its usefulness and decades of development, the amplifiers required for patch clamping are expensive and bulky. This has limited the scalability and throughput of patch clamping for single-ion channel and single-cell analyses. In this work, we have developed a custom patch-clamp amplifier microchip that can be fabricated using standard commercial silicon processes capable of performing both voltage- and current-clamp measurements. A key innovation is the use of nonlinear feedback elements in the voltage-clamp amplifier circuit to convert measured currents into logarithmically encoded voltages, thereby eliminating the need for large high-valued resistors, a factor that has limited previous attempts at integration. Benchtop characterization of the chip shows low levels of current noise [1.1 pA root mean square (rms) over 5 kHz] during voltage-clamp measurements and low levels of voltage noise (8.2 μV rms over 10 kHz) during current-clamp measurements. We demonstrate the ability of the chip to perform both current- and voltage-clamp measurement in vitro in HEK293FT cells and cultured neurons. We also demonstrate its ability to perform in vivo recordings as part of a robotic patch-clamping system. The performance of the patch-clamp amplifier microchip compares favorably with much larger commercial instrumentation, enabling benchtop commoditization, miniaturization, and scalable patch-clamp instrumentation. PMID:25429119

  12. Modeling of SVM Diode Clamping Three-Level Inverter Connected to Grid

    DEFF Research Database (Denmark)

    Guo, Yougui; Zeng, Ping; Zhu, Jieqiong;

    2011-01-01

    PLECS is used to model the diode clamping three-level inverter connected to grid and good results are obtained. First the output voltage SVM is described for diode clamping three-level inverter with loads connected to Y. Then the output voltage SVM of diode clamping three-level inverter is simply...... very powerful tool to real power circuits and it is very easy to simulate them. They have also verified that SVM control strategy is feasible to control the diode clamping three-level inverter....

  13. Cryogenic Clamp-on Ultrasonic Flowmeters using Single Crystal Piezoelectric Transducers Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Clamp-on ultrasound cryogenic flowmeters using single crystal piezoelectric transducers are proposed to enable reliable, accurate cryogenic instrumentation needs in...

  14. Effects of Environmental Medium and Surface Structure on the Friction Performance of CT Clamping Block%环境介质及表面结构对夹持块摩擦性能的影响

    Institute of Scientific and Technical Information of China (English)

    任武; 袁文才; 谭文锋; 徐云喜; 刘鑫; 李世龙

    2012-01-01

    连续管注入头夹持块在使用过程中表面会粘附钻井液、原油和石蜡等井下介质,从而对夹持块摩擦性能造成影响,另外夹持块表面结构形式的不同也会对摩擦性能造成影响。为此,用自行设计的测试设备对夹持块与连续管在不同条件下的当量摩擦因数进行测试及分析。分析结果表明,对于光面夹持块,在无介质条件下,其当量摩擦因数为0.443;原油对当量摩擦因数影响较小,石蜡对夹持块摩擦性能影响最大,其当量摩擦因数下降至0.074;对于无介质环境下的表面刻槽夹持块,齿宽和槽宽越窄,夹持性能越好;在石蜡环境下,23型夹持块有效减少了接触面上的石蜡,摩擦性能大幅度提高。%There are adhesion downhole media on the CT clamping block, such as slurry, crude oil and paraffin in its application process. As a result, the friction performance of the block will be affected. Moreover, the difference in the surface structure of the block will also have effect on its friction performance. Therefore, the self-designed testing apparatus was used to test and analyze the equivalent friction coefficient of the block and CT in different conditions. The findings show that as for smooth surface clamping block, the equivalent coefficient is 0. 443 when there is no medium. Crude oil has slight effect on the coefficient. Paraffin has the maximum effect on the friction performance of the block and the coefficient decreases to 0. 074. As for the medium - free clamping block with surface notch groove, the narrower the tooth width and groove width, the higher the clamping performance. In the environment of paraffin Model 23 clamping block effectively reduces the paraffin on the contact surface and the friction performance increases remarkably.

  15. Self-clamping arc light reflector for welding torch

    Science.gov (United States)

    Gordon, Stephen S.

    1987-07-01

    This invention is directed to a coaxial extending metal mirror reflector attached to the electrode housing or gas cup on a welding torch. An electric welding torch with an internal viewing system for robotic welding is provded with an annular arc light reflector to reflect light from the arc back onto the workpiece. The reflector has a vertical split or gap in its surrounding wall to permit the adjacent wall ends forming the split to be sprung open slightly to permit the reflector to be removed or slipped onto the torch housing or gas cup. The upper opening of the reflector is slightly smaller than the torch housing or gas cup and therefore, when placed on the torch housing or gas cup has that springiness to cause it to clamp tightly on the housing or gas cup. The split or gap also serves to permit the feed of weld wire through to the weld area,

  16. Trans-Z-source Neutral Point Clamped inverter

    DEFF Research Database (Denmark)

    Mo, W.; Loh, P. C.; Li, D.;

    2012-01-01

    Transformer based Z-source (trans-Z-source) inverters are recently proposed by extending the traditional Z-source inverter with higher buck-boost capability as well as reducing the passive components at the same time. Multi-Level Z-source inverters are single-stage topological solutions used for...... buck-boost energy conversion with all the favourable advantages of multi-level switching retained. This paper presents three-level trans-Z-source Neutral Point Clamped (NPC) inverter topology, which achieves both the advantages of trans-Z-source and three-level NPC inverter configuration. With proper...... modulation scheme, the three-level trans-Z-source inverter can function with minimum of six device commutations per half carrier cycle (same as the traditional buck NPC inverter), while maintaining to produce the designed volt-sec average and inductive voltage boosting at ac output terminals. The designed...

  17. Whole-cell Patch-clamp Recordings in Brain Slices.

    Science.gov (United States)

    Segev, Amir; Garcia-Oscos, Francisco; Kourrich, Saïd

    2016-01-01

    Whole-cell patch-clamp recording is an electrophysiological technique that allows the study of the electrical properties of a substantial part of the neuron. In this configuration, the micropipette is in tight contact with the cell membrane, which prevents current leakage and thereby provides more accurate ionic current measurements than the previously used intracellular sharp electrode recording method. Classically, whole-cell recording can be performed on neurons in various types of preparations, including cell culture models, dissociated neurons, neurons in brain slices, and in intact anesthetized or awake animals. In summary, this technique has immensely contributed to the understanding of passive and active biophysical properties of excitable cells. A major advantage of this technique is that it provides information on how specific manipulations (e.g., pharmacological, experimenter-induced plasticity) may alter specific neuronal functions or channels in real-time. Additionally, significant opening of the plasma membrane allows the internal pipette solution to freely diffuse into the cytoplasm, providing means for introducing drugs, e.g., agonists or antagonists of specific intracellular proteins, and manipulating these targets without altering their functions in neighboring cells. This article will focus on whole-cell recording performed on neurons in brain slices, a preparation that has the advantage of recording neurons in relatively well preserved brain circuits, i.e., in a physiologically relevant context. In particular, when combined with appropriate pharmacology, this technique is a powerful tool allowing identification of specific neuroadaptations that occurred following any type of experiences, such as learning, exposure to drugs of abuse, and stress. In summary, whole-cell patch-clamp recordings in brain slices provide means to measure in ex vivo preparation long-lasting changes in neuronal functions that have developed in intact awake animals

  18. HIRE seismic reflection survey in the Olkiluoto area

    International Nuclear Information System (INIS)

    A seismic reflection survey comprising three vibroseismic lines (total length of 31.1 km) was carried out in the Olkiluoto area, western Finland, in July, 2008. The survey is a part of the project HIRE (High Resolution Reflection Seismics for Ore Exploration 2007-2010) of the Geological Survey of Finland (GTK). The Olkiluoto survey was done in co-operation with Posiva Oy. The HIRE seismic reflection survey in the Olkiluoto area revealed numerous previously unknown structures in the upper crust of the area. The most prominent structures observed are the subhorizontal strong reflectors which very probably represent Postjotnian diabase sills intruding both the Svecofennian gneisses as well as the rapakivi granites. These reflectors can be associated with the similar seismic structures recorded in marine seismic transects in the Bothnian Sea, and thus they represent a large-scale structure. The Mesoproterozoic rapakivi granites can be distinguished as homogeneous, seismically transparent domains which extend to a depth of at least 4 km. The interpreted rapakivi structures are in a good agreement with gravity modellings. On the Olkiluoto Island, reflectors could be correlated with drillhole based data on lithology and brittle fault zones. The main brittle fault zones detected in drillholes are represented as reflectors in the seismic sections, and several new structures have been interpreted. A synthetic seismogram constructed for a 1 km deep hole in the Olkiluoto Island using down-hole logs of density and P-wave velocity, suggests that the main brittle fault zones generate strong reflectors. Pegmatitic granite sometimes has a weak reflection contrast with the surrounding gneisses, and sometimes has no contrast. There seems to be a simultaneous correlation of reflectivity with fracture zones at the location of the pegmatitic granite occurrences. The HIRE 2D seismic reflection data agrees well with the earlier 3D reflection surveys in the Olkiluoto Island, and most of

  19. Source Mechanism, Stress Triggering, and Hazard Analysis of Induced Seismicity in Oil/Gas Fields in Oman and Kuwait

    Science.gov (United States)

    Gu, C.; Toksoz, M. N.; Ding, M.; Al-Enezi, A.; Al-Jeri, F.; Meng, C.

    2015-12-01

    Induced seismicity has drawn new attentions in both academia and industry in recent years as the increasing seismic activity in the regions of oil/gas fields due to fluid injection/extraction and hydraulic fracturing. Source mechanism and triggering stress of these induced earthquakes are of great importance for understanding their causes and the physics of the seismic processes in reservoirs. Previous research on the analysis of induced seismic events in conventional oil/gas fields assumed a double couple (DC) source mechanism. The induced seismic data in this study are from both Oman and Kuwait. For the Oman data, the induced seismicity is monitored by both surface network (0oil/gas field. The data used in the study consist of 800 events located by the surface network and 2000 events from the downhole network. For the Kuwait data a surface network is used to collect the local seismic data (0oil/gas fields from year 1999 to 2007 for Oman, and from year 2006 to 2015 for Kuwait. In addition, the local hazard corresponding to the induced seismicity in these oil/gas fields is assessed and compared to ground motion prediction due to large (M>5.0) regional tectonic earthquakes.

  20. Advanced Data Communications for Downhole Data Logging and Control Applications in the Oil Industry

    International Nuclear Information System (INIS)

    We present details of 'Mercury', a high-speed downhole communications system that utilizes the (metallic) wall of a gas or oil pipeline or a drill 'string' as the communications 'channel' to control or monitor equipment or sensors used in the oil industry. Conventional downhole communication systems typically use 'mud pulse' telemetry for 'Measurement While Drilling' (MWD) operations. Current mud pulse telemetry technology offers bandwidths of up to 40 bit/s. However the data rate drops with increasing length of the wellbore and is typically as low as 1.5 bit/s – 3.0 bit/s at a depth of 35,000 ft. – 40,000 ft. The system described, by contrast, offers data rates of several megabits per second over distances of many kilometres and uses Orthogonal Frequency Division Multiplexing (OFDM) coupled with Wideband Frequency Division Multiple Access (W-CDMA). This paper presents details of our system; results of several trials undertaken on actual gas pipelines in the UK will be presented at the Conference

  1. Flow measurement and characterization in shallow geothermal systems used for downhole heat exchanger applications

    Energy Technology Data Exchange (ETDEWEB)

    Churchill, D.; Culver, G.G.; Reistad, G.M.

    1977-01-01

    In the largest non-electrical application of geothermal energy presently occurring in the United States, over 400 relatively shallow wells are being used for extraction of energy with downhole heat exchangers. Despite this large amount of application, the exact nature of the flows in the wells has not before been characterized. Knowledge to date on the nature of flows in the systems is summarized, and an ongoing experimental program for making appropriate downhole measurements to determine flows is described in detail. Flow characterization was a principal object of this study. Horizontal cross-flows of geothermal fluid may occur at upper and/or lower levels in the well where perforations in the well casing are situated. In addition, natural convection may induce vertical flows within the well casing which would be influenced by the presence or absence of a heat exchanger. Three main aspects of the experimental program are reported on: (i) a review of potentially applicable methods for measuring vertical and horizontal flows in wells, (ii) the limitations and preliminary results of using a vane anemometer for measuring vertical flows, and (iii) the description of the selected hot-film probe, its associated pressurized calibration facility, and means of making well measurements.

  2. Optical instruments for a combined seismic and geodetic borehole observatory

    Science.gov (United States)

    Zumberge, Mark; Agnew, Duncan; Berger, Jonathan; Hatfield, William; Wyatt, Frank

    2016-04-01

    Optical interferometry offers displacement sensing with the unusual combination of high sensitivity, linearity, and wide dynamic range, and it can be adapted to high temperature environments. We have applied interferometric technology to inertial seismic instruments and to optical fibers for strain measurements. When combining these methods into a single borehole package the result is a system that provides three components of observatory quality seismic recordings, two components of tilt, gravity, and vertical strain. The borehole package is entirely passive with the need for only optical fibers to connect the sensor sonde with surface electronics. One of the sensors in the system is an optical fiber strainmeter, which consists of an optical fiber cable elastically stretched between two borehole anchor points separated by 100 m or more. The fiber's length is recorded optically, enabling sub-nanostrain detection of crustal deformations. A second sensor system uses laser interferometry to record the displacements of inertial mechanical suspensions - spring-mass for the vertical component and pendulums for the horizontal components - housed in a borehole sonde. The combined system is able to measure vertical and horizontal ground velocities, gravity, and tilt with sensitivities that compare favorably with any existing borehole system over time scales from 10 Hz to many days; because the downhole components are entirely passive, the instrument will have a long lifetime and could be made usable at high downhole temperatures. The simplicity and longevity of the metal and glass borehole sonde make it suitable for permanent cementation into a borehole to achieve good coupling and stability. Several versions of the borehole inertial system have been deployed on land with excellent results, and a number of our optical fiber strainmeters have been deployed - both onshore and offshore. The combined system is currently under development.

  3. Geology of the Waste Treatment Plant Seismic Boreholes

    Energy Technology Data Exchange (ETDEWEB)

    Barnett, D. BRENT; Bjornstad, Bruce N.; Fecht, Karl R.; Lanigan, David C.; Reidel, Steve; Rust, Colleen F.

    2007-02-28

    In 2006, DOE-ORP initiated the Seismic Boreholes Project (SBP) to emplace boreholes at the Waste Treatment Plant (WTP) site in order to obtain direct Vs measurements and other physical property measurements in Columbia River basalt and interbedded sediments of the Ellensburg Formation. The goal was to reduce the uncertainty in the response spectra and seismic design basis, and potentially recover design margin for the WTP. The characterization effort within the deep boreholes included 1) downhole measurements of the velocity properties of the suprabasalt, basalt, and sedimentary interbed sequences, 2) downhole measurements of the density of the subsurface basalt and sediments, and 3) confirmation of the geometry of the contact between the various basalt and interbedded sediments through examination of retrieved core from the corehole and data collected through geophysical logging of each borehole. This report describes the results of the geologic studies from three mud-rotary boreholes and one cored borehole at the WTP. All four boreholes penetrated the entire Saddle Mountains Basalt and the upper part of the Wanapum Basalt where thick sedimentary interbeds occur between the lava flows. The basalt flows penetrated in Saddle Mountains Basalt included the Umatilla Member, Esquatzel Member, Pomona Member and the Elephant Mountain Member. The underlying Priest Rapids Member of the Wanapum Basalt was also penetrated. The Ellensburg Formation sediments consist of the Mabton Interbed, the Cold Creek Interbed, the Selah Interbed and the Rattlesnake Ridge Interbed; the Byron Interbed occurs between two flows of the Priest Rapids Member. The Mabton Interbed marks the contact between the Wanapum and Saddle Mountains Basalts. The thicknesses of the basalts and interbedded sediments were within expected limits. However, a small reverse fault was found in the Pomona Member flow top. This fault has three periods of movement and less than 15 feet of repeated section. Most of the

  4. 3D seismic data for shallow aquifers characterisation

    Science.gov (United States)

    Giustiniani, Michela; Accaino, Flavio; Picotti, Stefano; Tinivella, Umberta

    2009-07-01

    We present the results obtained from conventional and non-conventional analysis of 3D high-resolution seismic data acquired nearby the water spring line, which separates the upper from the lower Friuli-Venezia Giulia plain (Italy), in order to characterise an important multilayered confined aquifer. The main targets of this study were two shallow aquifers located at about 30 m and 180 m depth, respectively. The aquifer structures were reconstructed by adopting a technique consisting an iterative updating procedure, for refining and improving an initial model in depth. The method includes pre-stack depth migration, residual move-out analysis and seismic reflection tomography. In the final 3D migrated cubes, two high velocity layers were identified at about 270 m and 480 m respectively, which correspond to unknown deep aquifers, as confirmed by recent well data (stratigraphies and down-hole velocity measurements). Travel-time tomography and Amplitude Versus Offset analysis evidence that seasonal variation in the seismic response of the aquifers are not detectable. However, in this case, aquifers are well detectable by lithological changes.

  5. Evaluation of selected martensitic stainless steels for use in downhole tubular expansion - Results of a laboratory study

    Energy Technology Data Exchange (ETDEWEB)

    Mack, Robert [Shell International E and P, b.v. Kessler Park 1, Postbus 60, 2280 AB Rijswijk (Netherlands)

    2004-07-01

    A laboratory program was performed to evaluate the potential of selected martensitic stainless steels for downhole cladding applications. The evaluation of the effects of tubular expansion on mechanical properties, defects, and resistance to environmentally assisted cracking demonstrated that some steels were acceptable for the intended application. The results were used to qualify and select the stainless steel for the intended sweet cladding applications. (authors)

  6. The deformation of an elastic rod with a clamp sliding along a smooth and curved profile

    CERN Document Server

    Misseroni, D; Zaccaria, D; Bigoni, D

    2015-01-01

    The design of compliant mechanisms is crucial in several technologies and relies on the availability of solutions for nonlinear structural problems. One of these solutions is given and experimentally validated in the present article for a compliant mechanism moving along a smooth curved profile. In particular, a deformable elastic rod is held by two clamps, one at each end. The first clamp is constrained to slide without friction along a curved profile, while the second clamp moves in a straight line transmitting its motion through the elastic rod to the first clamp. For this system it is shown that the clamp sliding on the profile imposes nontrivial boundary conditions (derived via a variational and an asymptotic approach), which strongly influence buckling and nonlinear structural behaviour. Investigation of this behaviour shows that a compliant mechanism can be designed, which gives an almost neutral response in compression. This behavior could easily be exploited to make a force limiting device. Finally a...

  7. A reduction clamp for an aiming component in associated acetabular fractures

    Directory of Open Access Journals (Sweden)

    Zhang-Fu Wang

    2015-01-01

    Full Text Available Background: The treatment of acetabular fractures is complex and requires specialized equipment. However, all currently available instruments have some disadvantages. A new reduction clamp that can firmly enable reduction and not hinder subsequent fixation procedures for some special fracture types is needed. Materials and Methods: In this study, we introduce a new acetabular clamp and its preliminary clinical application in three T-shaped acetabular fractures. Results: This new clamp can successfully pull the posterior column back to the anterior column and firmly maintain the reduction. This clamp′s aiming plate can facilitate the insertion of long lag screws. The clamp is also easy to assemble and use. Conclusion: This reduction clamp is a useful instrument that can facilitate open reduction and internal fixation of acetabular fractures.

  8. Clamp ultrastructure of the basal monogenean Chimaericola leptogaster (Leuckart, 1830) (Polyopisthocotylea: Chimaericolidae).

    Science.gov (United States)

    Poddubnaya, Larisa G; Hemmingsen, Willy; Gibson, David I

    2014-11-01

    The ultrastructure of the haptoral clamps of the chimaericolid monogenean Chimaericola leptogaster, a basal polyopisthocotylean from the gills of a holocephalan fish, is described. These clamps are characterized by the presence of two muscle blocks interrupted mid-anteriorly and mid-posteriorly and different kinds of hard structures: a single median and paired lateral sclerites embedded in the clamp wall; six spine-like structures directed towards the clamp lumen; and electron dense surface structures along the internal surface of the anterior clamp lips and along the luminal surface of the tegument of the clamp lumen. The lateral sclerites are situated deep within muscular tissue and are closely bounded by radial myofibrils, possessing a uniform electron dense matrix within which are hollow areas of different sizes. The median sclerite occupies an area between the clamp wall myofibrils and the luminal epithelium, is surrounded by a basement lamina and is composed of a heterogeneous matrix comprising two different morphological layers related to variations in the type and concentration of fibrils. Four of the spine-like structures are extensions of the margins of the two spindle-like muscle blocks in the clamps, i.e. the two anterior and two posterior structures, and the two others are situated at the lateral constrictions of the left and right muscle blocks. The electron dense surface structures are derivations of the clamp tegument or, to be more precise, its outer, densely fibrous region. These results are discussed in relation to the evidence that the haptoral clamps of C. leptogaster are apparently ancient origin. PMID:25112214

  9. Studies on seismic waves

    Institute of Scientific and Technical Information of China (English)

    张海明; 陈晓非

    2003-01-01

    The development of seismic wave study in China in the past four years is reviewed. The discussion is divided into several aspects, including seismic wave propagation in laterally homogeneous media, laterally heterogeneous media, anisotropic and porous media, surface wave and seismic wave inversion, and seismic wave study in prospecting and logging problems. Important projects in the current studies on seismic wave is suggested as the development of high efficient numerical methods, and applying them to the studies of excitation and propagation of seismic waves in complex media and strong ground motion, which will form a foundation for refined earthquake hazard analysis and prediction.

  10. Preliminary analysis of downhole logging data from ICDP Lake Junin drilling Project, Peru

    Science.gov (United States)

    Pierdominici, Simona; Kück, Jochem; Rodbell, Donald T.; Abbott, Mark B.

    2016-04-01

    The International Continental Drilling Programm (ICDP) has supported a scientific drilling campaign in Peru during the summer season 2015. The Lake Junin Drilling Project mainly aims at obtaining high-resolution paleoclimate records from lacustrine sediments to reconstruct the history of the continental records covering the glacial-interglacial cycles. Lake Junín is located at 4000 m a.s.l. in the tropical Andes of Peru, and is characterized by a thick (> 125 m) sediment package deposited at a high rate (0.2 to 1.0 mm yr-1). Lake Junín is one of the few lakes in the tropical Andes that predates the maximum extent of glaciation and is in a geomorphic position to record the waxing and waning of glaciers in nearby cordillera, hence making the lake a key site for the investigation of the Quaternary climate evolution in the inner-tropics of the Southern Hemisphere. Continous coring was performed at three sites in overall 11 boreholes on the lake with at least two overlapping boreholes per site to avoid core gaps. The depth of the boreholes varied between approx. 30 m and 110 m depending on the drill site. The core bit had a bit size of 122.6 mm and yielded a core diameter of 85 mm. Upon completion of coring operations downhole geophysical logging was performed in five of the 11 boreholes (1A, 1C, 1D, 2A and 3B) by the Operational Support Group of ICDP. The main objective was to record in-situ the physical properties of the lacustrine sediments of Lake Junin. Downhole logs provide a powerful tool to fill in information at intervals with core gaps and as depth reference for depth matching of the discontinous cores. Furthermore it will be used for the lithological reconstruction and interpretation. The OSG downhole logging comprised total and spectrum gamma ray, magnetic susceptibility, borehole geometry, temperature, and sonic P-wave velocity. Unstable and collapsing borehole walls made it neccessary to carry out logging in several sections instead of in one run. The

  11. Impacts of shaking Bengkulus seismicity to subsurface Wellbore in the XX area

    Science.gov (United States)

    Rusli, Saifatur; Pratama, M. Ardian; Mardiyan, Hilman; Mirza, Finisha, Brian

    2016-05-01

    Bengkulu earthquake was happened on Wednesday, May 5th 2010 at 23:29:03 WIB (UTC-7) with magnitude 6.5 Richter scale. The Epicentrum was very close to Mentawai Fault System located on latitude 4.063°S and longitude 101.085°E with depth subsurface 27 kms. It was occurred the result of the Indian Ocean Plate-Australia's activity with low angled-subducted beneath the island of Sumatra. Shaking Bengkulus seismicity impacted subsurface in the XX Area which situated on South Palembang Sub-Basin part of South Sumatra Basin (SSB) about 200 kms far away from the epicentrum. Due to XX Area has some producing wells so that the seismicity activities as hypothetically impacts to Subsurface Wellbore which has caused some wells casing problems in the same depth. The wells casing problem shown after conducted routine sand bailer by Sand Line Unit, it was a downhole device used to remove debris sands or similar small particles around the fishingnecks of downhole tools or equipment in the wellbore, and then Sand Bailer tools got scratch at one side body. Similarly, Sand Bailer tools couldnt lowered until Total Depth and got samples sands slightly. At the end, it has impacted to well performance to produce oil in the XX Area.

  12. Voltage clamping single cells in intact malpighian tubules of mosquitoes.

    Science.gov (United States)

    Masia, R; Aneshansley, D; Nagel, W; Nachman, R J; Beyenbach, K W

    2000-10-01

    Principal cells of the Malpighian tubule of the yellow fever mosquito were studied with the methods of two-electrode voltage clamp (TEVC). Intracellular voltage (V(pc)) was -86.7 mV, and input resistance (R(pc)) was 388.5 kOmega (n = 49 cells). In six cells, Ba(2+) (15 mM) had negligible effects on V(pc), but it increased R(pc) from 325.3 to 684.5 kOmega (P usefulness of TEVC methods in single principal cells of the intact Malpighian tubule. Dinitrophenol (0.1 mM) reversibly depolarized V(pc) from -94.3 to -10.7 mV (P < 0.001) and reversibly increased R(pc) from 412 to 2,879 kOmega (P < 0.001), effects that were duplicated by cyanide (0.3 mM). Significant effects of metabolic inhibition on voltage and resistance suggest a role of ATP in electrogenesis and the maintenance of conductive transport pathways. PMID:10997925

  13. Analysis of Control Strategies for Diode Clamped Multilevel Inverter

    Directory of Open Access Journals (Sweden)

    C. R. Balamurugan

    2013-05-01

    Full Text Available This paper presents the comparison of various Pulse Width Modulation (PWM strategies for the three phase Diode Clamped Multi Level Inverter (DCMLI. The main contribution of this paper is the proposal of new modulation schemes with Variable Amplitude (VA and various new schemes adopting the constant switching frequency and also variable switching frequency multicarrier control freedom degree combination concepts are developed and simulated for the chosen three phase DCMLI. The three phase DCMLI, is controlled in this paper with Sinusoidal PWM (SPWM reference along with triangular carriers and analysis is made among both without carrier overlapping and with Carrier Overlapping (CO techniques to choose the better strategy by performing simulation using MATLAB-SIMULINK. The variation of Total Harmonic Distortion (THD and fundamental RMS output voltage is observed for various modulation indices. It is observed that among the various equal amplitude PWM strategies, COPWM-C provides less THD and higher RMS voltage. It is recognized that among the various variable amplitude PWM strategies, VACOPWM-C provides less THD and VACOPWM-B provides higher RMS voltage. By comparing the equal amplitude PWM strategies with the variable amplitude PWM strategies it is inferred that VACOPWM-C provides less THD and VACOPWM-B provides higher RMS voltage. It is also inferred that carrier overlapping techniques provides better results compared to the without carrier overlapping techniques.

  14. Position clamping in a holographic counterpropagating optical trap.

    Science.gov (United States)

    Bowman, Richard; Jesacher, Alexander; Thalhammer, Gregor; Gibson, Graham; Ritsch-Marte, Monika; Padgett, Miles

    2011-05-01

    Optical traps consisting of two counterpropagating, divergent beams of light allow relatively high forces to be exerted along the optical axis by turning off one beam, however the axial stiffness of the trap is generally low due to the lower numerical apertures typically used. Using a high speed spatial light modulator and CMOS camera, we demonstrate 3D servocontrol of a trapped particle, increasing the stiffness from 0.004 to 1.5 μN m(-1). This is achieved in the "macro-tweezers" geometry [Thalhammer, J. Opt. 13, 044024 (2011); Pitzek, Opt. Express 17, 19414 (2009)], which has a much larger field of view and working distance than single-beam tweezers due to its lower numerical aperture requirements. Using a 10×, 0.2 NA objective, active feedback produces a trap with similar effective stiffness to a conventional single-beam gradient trap, of order 1 μN m(-1) in 3D. Our control loop has a round-trip latency of 10 ms, leading to a resonance at 20 Hz. This is sufficient bandwidth to reduce the position fluctuations of a 10 μm bead due to Brownian motion by two orders of magnitude. This approach can be trivially extended to multiple particles, and we show three simultaneously position-clamped beads. PMID:21643247

  15. Downhole instrumentation for the evaluation of non-linear soil response on ground surface motion

    International Nuclear Information System (INIS)

    A downhole experiment at McGee Creek in eastern central California has shown that, for hard soil deposits, the one-dimensional shear-wave model for the soil constitutive relationship is adequate for use in the computation of ground surface motions. In order to investigate the influence of soil non-linearities on these latter, an alluvial site has been instrumented in southern California, at Garner Valley. This paper presents the characterization of the site and the soil parameters derived from in-site and laboratory-based tests. Preliminary results for the recorded ground motion responses corresponding to small-magnitude earthquakes are compared with computed motions from a one-dimensional shear-wave model. (author)

  16. Benefits and technical challenges of downhole steam generation for enhanced oil recovery

    Energy Technology Data Exchange (ETDEWEB)

    Castrogiovanni, A.; Fitzpatrick, N.; Ware, C. H. [Society of Petroleum Engineers (Canada)

    2011-07-01

    The use of steam is a popular method in heavy oil recovery. Steam is typically generated at the surface by once-through steam generators and is pumped via pipes into the ground. While it is relatively easy to insulate the pipes at the surface, the opposite holds true when the pipes enter the ground. There is a high level of heat loss even in tubing that is surrounded by a vacuum. This limits maximum operational depth of the well to 2500ft. This paper suggests that the use of downhole steam generators (DHSG) would allow operators to reach oil deposits much deeper in the earth. The cost of the DHSG technology is economically viable at 2009 oil prices. There are certain technical challenges that need to be overcome with this method. Since the DHSG method does not heat up the earth between the surface and the deposit it has the potential to be applied in environmentally sensitive areas.

  17. ENHANCED OIL RECOVERY WITH DOWNHOLE VIBRATION STIMULATION IN OSAGE COUNTY OKLAHOMA

    Energy Technology Data Exchange (ETDEWEB)

    Robert Westermark; J. Ford Brett

    2003-11-01

    This Final Report covers the entire project from July 13, 2000 to June 30, 2003. The report summarizes the details of the work done on the project entitled ''Enhanced Oil Recovery with Downhole Vibration Stimulation in Osage County Oklahoma'' under DOE Contract Number DE-FG26-00BC15191. The project was divided into nine separate tasks. This report is written in an effort to document the lessons learned during the completion of each task. Therefore each task will be discussed as the work evolved for that task throughout the duration of the project. Most of the tasks are being worked on simultaneously, but certain tasks were dependent on earlier tasks being completed. During the three years of project activities, twelve quarterly technical reports were submitted for the project. Many individual topic and task specific reports were included as appendices in the quarterly reports. Ten of these reports have been included as appendices to this final report. Two technical papers, which were written and accepted by the Society of Petroleum Engineers, have also been included as appendices. The three primary goals of the project were to build a downhole vibration tool (DHVT) to be installed in seven inch casing, conduct a field test of vibration stimulation in a mature waterflooded field and evaluate the effects of the vibration on both the produced fluid characteristics and injection well performance. The field test results are as follows: In Phase I of the field test the DHVT performed exceeding well, generating strong clean signals on command and as designed. During this phase Lawrence Berkeley National Laboratory had installed downhole geophones and hydrophones to monitor the signal generated by the downhole vibrator. The signals recorded were strong and clear. Phase II was planned to be ninety-day reservoir stimulation field test. This portion of the field tests was abruptly ended after one week of operations, when the DHVT became stuck in the well

  18. Theoretical background and the flow fields in downhole liquid-liquid hydrocyclone (LLHC

    Directory of Open Access Journals (Sweden)

    Osei Harrison

    2014-07-01

    Full Text Available Hydrocyclone system for downhole oil-water separation provides an effective technique of enhancing the economic viability of higher water-cut wells while at the same time reducing the risk of environmental pollution. This paper describes the hydrodynamics of the liquid-liquid hydrocyclones and the flow fields within it are paramount for achieving successful separation process. Some of the important hydrodynamic flow phenomenon within the liquid-liquid hydrocyclone and how they influence the separation efficiency of water/oil was analyzed through analytical solution. The properties of the liquids were based on Bayan offshore field measured properties. The results indicated that there are two swirling zones separated by stagnant flow field. The inner is the light liquid zone, while the outer is the heavy liquid zone.

  19. Downhole fluid injection systems, CO2 sequestration methods, and hydrocarbon material recovery methods

    Science.gov (United States)

    Schaef, Herbert T.; McGrail, B. Peter

    2015-07-28

    Downhole fluid injection systems are provided that can include a first well extending into a geological formation, and a fluid injector assembly located within the well. The fluid injector assembly can be configured to inject a liquid CO2/H2O-emulsion into the surrounding geological formation. CO2 sequestration methods are provided that can include exposing a geological formation to a liquid CO2/H2O-emulsion to sequester at least a portion of the CO2 from the emulsion within the formation. Hydrocarbon material recovery methods are provided that can include exposing a liquid CO2/H2O-emulsion to a geological formation having the hydrocarbon material therein. The methods can include recovering at least a portion of the hydrocarbon material from the formation.

  20. Downhole fluid injection systems, CO2 sequestration methods, and hydrocarbon material recovery methods

    Energy Technology Data Exchange (ETDEWEB)

    Schaef, Herbert T.; McGrail, B. Peter

    2015-07-28

    Downhole fluid injection systems are provided that can include a first well extending into a geological formation, and a fluid injector assembly located within the well. The fluid injector assembly can be configured to inject a liquid CO2/H2O-emulsion into the surrounding geological formation. CO2 sequestration methods are provided that can include exposing a geological formation to a liquid CO2/H2O-emulsion to sequester at least a portion of the CO2 from the emulsion within the formation. Hydrocarbon material recovery methods are provided that can include exposing a liquid CO2/H2O-emulsion to a geological formation having the hydrocarbon material therein. The methods can include recovering at least a portion of the hydrocarbon material from the formation.

  1. Downhole fiber optic sensing: the oilfield service provider's perspective: from the cradle to the grave

    Science.gov (United States)

    Skinner, Neal G.; Maida, John L.

    2014-06-01

    For almost three decades, interest has continued to increase with respect to the application of fiber-optic sensing techniques for the upstream oil and gas industry. This paper reviews optical sensing technologies that have been and are being adopted downhole, as well as their drivers. A brief description of the life of a well, from the cradle to the grave, and the roles fiber-optic sensing can play in optimizing production, safety, and protection of the environment are also presented. The performance expectations (accuracy, resolution, stability, and operational lifetime) that oil companies and oil service companies have for fiber-optic sensing systems is described. Additionally, the environmental conditions (high hydrostatic pressures, high temperatures, shock, vibration, crush, and chemical exposure) that these systems must tolerate to provide reliable and economically attractive oilfield monitoring solutions are described.

  2. Harsh-Environment Solid-State Gamma Detector for Down-hole Gas and Oil Exploration

    Energy Technology Data Exchange (ETDEWEB)

    Peter Sandvik; Stanislav Soloviev; Emad Andarawis; Ho-Young Cha; Jim Rose; Kevin Durocher; Robert Lyons; Bob Pieciuk; Jim Williams; David O' Connor

    2007-08-10

    The goal of this program was to develop a revolutionary solid-state gamma-ray detector suitable for use in down-hole gas and oil exploration. This advanced detector would employ wide-bandgap semiconductor technology to extend the gamma sensor's temperature capability up to 200 C as well as extended reliability, which significantly exceeds current designs based on photomultiplier tubes. In Phase II, project tasks were focused on optimization of the final APD design, growing and characterizing the full scintillator crystals of the selected composition, arranging the APD device packaging, developing the needed optical coupling between scintillator and APD, and characterizing the combined elements as a full detector system preparing for commercialization. What follows is a summary report from the second 18-month phase of this program.

  3. Holy grail: Pioneering acoustic telemetry technology set to revolutionize downhole communication

    Energy Technology Data Exchange (ETDEWEB)

    Greenaway, R.

    2003-12-01

    Acoustic telemetry, a faster and more efficient downhole-to-surface-communication technology, is the latest development in downhole communication systems. The system has been developed by Extreme Engineering Limited of Calgary, led by Derek Logan, founder and one-time senior vice-president of Ryan Energy Technologies that developed the original measurement -while-drilling (MWD) and logging-while-drilling )LWD) tools. The company predicts that acoustic telemetry will cause a massive transformation of the drilling industry in Western Canada once the technology is commercialized. Conventional MWD techniques, based on mud-pulse technology, have been industry standard since the 1970s, but mud-pulse technology is now considered extremely slow. In the 1980s industry came up electromagnetic telemetry, as an alternative to mud-pulse. Today, the need to transmit ever more data, the need for a faster communications system and greater wellbore control, has become even more pressing. Logan believes that acoustic technology is the answer. It is not only capable of transmitting data 20 to 30 times faster than mud-pulse telemetries, it can also communicate massive amounts of data. It can be used in drilling, completion production, drillstem testing, frac monitoring and any other wellbore process requiring wireless real-time telemetry. Acoustic telemetry is also the only wireless system that can perform MWD and LWD in offshore underbalanced drilling. Notwithstanding its great promise, Extreme Engineering Limited had considerable difficulty raising funds for developing and commercializing XAcT (the trade name for acoustic telemetry). Prospects are reported to have been substantially improved by recent infusion of funds by the federal Industrial Research Assistance Program (IRAP) , and XAcT's recognition by R and D Magazine with one of the R and D 100 awards for 2003. 3 figs.

  4. Behavior of a hollow core photonic crystal fiber under high radial pressure for downhole application

    Energy Technology Data Exchange (ETDEWEB)

    Sadeghi, J., E-mail: j-sadeghi@sbu.ac.ir; Chenari, Z.; Ziaee, F. [Laser and Plasma Research Institute, Shahid Beheshti University, 1983963113 Tehran (Iran, Islamic Republic of); Latifi, H., E-mail: latifi@sbu.ac.ir [Laser and Plasma Research Institute, Shahid Beheshti University, 1983963113 Tehran (Iran, Islamic Republic of); Department of Physics, Shahid Beheshti University, Evin, 1983963113 Tehran (Iran, Islamic Republic of); Santos, J. L., E-mail: josantos@fc.up.pt [INESC Porto—Instituto de Engenharia de Sistemas e Computadores do Porto, Rua do Campo Alegre, 687, 4169-007 Porto (Portugal); Departamento de Física, da Faculdade de Ciências, da Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto (Portugal)

    2014-02-17

    Pressure fiber sensors play an important role in downhole high pressure measurements to withstand long term operation. The purpose of this paper is to present an application of hollow core photonic crystal fiber (HC-PCF) as a high pressure sensor head for downhole application based on dispersion variation. We used a high pressure stainless steel unit to exert pressure on the sensor. The experimental results show that different wavelengths based on sagnac loop interferometer have additive sensitivities from 5 × 10{sup −5} nm/psi at 1480 nm to 1.3 × 10{sup −3} nm/psi at 1680 nm. We developed a simulation to understand the reason for difference in sensitivity of wavelengths and also the relationship between deformation of HC-PCF and dispersion variation under pressure. For this purpose, by using the finite element method, we investigated the effect of structural variation of HC-PCF on spectral transformation of two linear polarizations under 1000 psi pressure. The simulation and experimental results show exponential decay behavior of dispersion variation from −3.4 × 10{sup −6} 1/psi to −1.3 × 10{sup −6} 1/psi and from −5 × 10{sup −6} 1/psi to −1.8 × 10{sup −6} 1/psi, respectively, which were in a good accordance with each other.

  5. Concentrations of plastic strains in the clamping region of cylindrical shells under excessive loading

    International Nuclear Information System (INIS)

    Different clamping conditions and variations of the material behaviour are considered. Especially the plastic strain concentrations at the shell clamping are determined. The analyses have been carried out with a finite-element code. Standard stress analysis procedures like the ASME-code are critically assessed. The results show that only minor plastic strain concentrations have to be expected for a rigidly clamped shell loaded by internal pressure. The work hardening characteristic of the material has little influence on these strain concentrations. On the other hand considerable plastic strain concentrations have to be expected at a flange type clamping loaded by axial stresses in the shell. These concentrations depend on the work hardening characteristic of the material. Comparison of these analyses with standard stress analysis procedures shows that these procedures are conservative, provided the classification of the different types of stresses has been done in a correct way which, however, may be difficult for some problems. For instance, for the rigidly clamped shell bending stresses can be classified as secondary stresses, but for a flange type clamping bending stresses must be treated as primary stresses with lower limits. With these results the structural integrity of the core barrel clamping of a PWR under blowdown loading has been assessed. Even in case of a sudden and complete pipe break the structure is able to withstand blowdown loading. (orig./HP)

  6. Seismic testing and analysis of a prototypic nonlinear piping system

    International Nuclear Information System (INIS)

    A series of seismic tests and analyses of a nonlinear Fast Flux Test Facility (FFTF) prototypic piping system are described, and measured responses are compared with analytical predictions. The test loop was representative of a typical LMFBR insulated small bore piping system and it was supported from a rigid test frame by prototypic dead weight supports, mechanical snubbers and pipe clamps. Various piping support configurations were tested and analyzed to evaluate the effects of free play and other nonlinear stiffness characteristics on the piping system response

  7. Modelling the clamping force distribution among chips in press-pack IGBTs using the finite element method

    DEFF Research Database (Denmark)

    Hasmasan, Adrian Augustin; Busca, Christian; Teodorescu, Remus;

    2012-01-01

    In this paper, a FEM (finite element method) based mechanical model for PP (press-pack) IGBTs (insulated gate bipolar transistors) is presented, which can be used to calculate the clamping force distribution among chips under various clamping conditions. The clamping force is an important parameter...

  8. Quantitative Seismic Amplitude Analysis

    NARCIS (Netherlands)

    Dey, A.K.

    2011-01-01

    The Seismic Value Chain quantifies the cyclic interaction between seismic acquisition, imaging and reservoir characterization. Modern seismic innovation to address the global imbalance in hydrocarbon supply and demand requires such cyclic interaction of both feed-forward and feed-back processes. Cur

  9. Robotization in Seismic Acquisition

    NARCIS (Netherlands)

    Blacquière, G.; Berkhout, A.J.

    2013-01-01

    The amount of sources and detectors in the seismic method follows "Moore’s Law of seismic data acquisition", i.e., it increases approximately by a factor of 10 every 10 years. Therefore automation is unavoidable, leading to robotization of seismic data acquisition. Recently, we introduced a new sour

  10. Voltage clamp of the cardiac sodium current at 37 degrees C in physiologic solutions.

    OpenAIRE

    Murray, K T; Anno, T.; Bennett, P B; Hondeghem, L M

    1990-01-01

    The cardiac sodium current was studied in guinea pig ventricular myocytes using the cell-attached patch voltage clamp at 37 degrees C in the presence of 145 mM external sodium concentration. When using large patch pipettes (access resistance, 1-2 M omega), the capacity current transient duration was typically 70 microseconds for voltage clamp steps up to 150 mV. At 37 degrees C the maximum inward sodium current peaked in approximately 200 microseconds after the onset of a clamp step and at th...

  11. Nuclear EMP: voltage clamping levels for several TransZorb transient suppression devices

    International Nuclear Information System (INIS)

    Voltage clamping levels for the 1N5629A, 1N5545A, 1N5654A, and 1N5664A TransZorb/sup T.M./ silicon transient-suppression devices in a DO-13 package are presented as data in photographs of test waveforms. A graph is compiled which illustrates the clamping voltage at a time point of 80 nsec for each of the devices. The duration of the test pulse was 250 nsec with a slope of 4 kV per nsec for the rise portion. Clamping levels are nearly constant for test current amplitudes of up to 120 A

  12. Analysis of the role of PCNA-DNA contacts during clamp loading

    Directory of Open Access Journals (Sweden)

    Goedken Eric R

    2010-01-01

    Full Text Available Abstract Background Sliding clamps, such as Proliferating Cell Nuclear Antigen (PCNA in eukaryotes, are ring-shaped protein complexes that encircle DNA and enable highly processive DNA replication by serving as docking sites for DNA polymerases. In an ATP-dependent reaction, clamp loader complexes, such as the Replication Factor-C (RFC complex in eukaryotes, open the clamp and load it around primer-template DNA. Results We built a model of RFC bound to PCNA and DNA based on existing crystal structures of clamp loaders. This model suggests that DNA would enter the clamp at an angle during clamp loading, thereby interacting with positively charged residues in the center of PCNA. We show that simultaneous mutation of Lys 20, Lys 77, Arg 80, and Arg 149, which interact with DNA in the RFC-PCNA-DNA model, compromises the ability of yeast PCNA to stimulate the DNA-dependent ATPase activity of RFC when the DNA is long enough to extend through the clamp. Fluorescence anisotropy binding experiments show that the inability of the mutant clamp proteins to stimulate RFC ATPase activity is likely caused by reduction in the affinity of the RFC-PCNA complex for DNA. We obtained several crystal forms of yeast PCNA-DNA complexes, measuring X-ray diffraction data to 3.0 Å resolution for one such complex. The resulting electron density maps show that DNA is bound in a tilted orientation relative to PCNA, but makes different contacts than those implicated in clamp loading. Because of apparent partial disorder in the DNA, we restricted refinement of the DNA to a rigid body model. This result contrasts with previous analysis of a bacterial clamp bound to DNA, where the DNA was well resolved. Conclusion Mutational analysis of PCNA suggests that positively charged residues in the center of the clamp create a binding surface that makes contact with DNA. Disruption of this positive surface, which had not previously been implicated in clamp loading function, reduces RFC

  13. Climate implications and lithological characteristics of sediments from Lake Van (Turkey) and Lake Ohrid (Macedonia / Albania) obtained from downhole logging data

    OpenAIRE

    Baumgarten, Henrike

    2015-01-01

    In this thesis, downhole logging data from Lake Van (Turkey) and Lake Ohrid (Macedonia/Albania) was analyzed to improve paleoenvironmental studies. The sediments of Lake Van (clayey silts and tephra) were differentiated by downhole logging and XRF scanning data. Spectral gamma ray (SGR), zirconium intensities and magnetic susceptibility are most useful to separate the lake facies from tephra. The U data from SGR was used for cyclostratigraphic studies to estimate the duration of deposition. T...

  14. Finite element analysis on factors influencing the clamping force in an electrostatic chuck

    International Nuclear Information System (INIS)

    As one of the core components of IC manufacturing equipment, the electrostatic chuck (ESC) has been widely applied in semiconductor processing such as etching, PVD and CVD. The clamping force of the ESC is one of the most important technical indicators. A multi-physics simulation software COMSOL is used to analyze the factors influencing the clamping force. The curves between the clamping force and the main parameters such as DC voltage, electrode thickness, electrode radius, dielectric thickness and helium gap are obtained. Moreover, the effects of these factors on the clamping force are investigated by means of orthogonal experiments. The results show that the factors can be ranked in order of voltage, electrode radius, helium gap and dielectric thickness according to their importance, which may offer certain reference for the design of ESCs. (semiconductor devices)

  15. BER improvement using optical gain clamped amplifier for burst transmission and critical cases studies

    OpenAIRE

    Zannin, Marcelo; Ennser, Karin; Taccheo, Stefano; Careglio, Davide; Solé Pareta, Josep; Aracil Rico, Javier

    2009-01-01

    This paper demonstrates significant BER improvement in burst traffic transmission using optical-gain clamped amplifiers. Critical chaotic cases are identified for peculiar burst sequences and their impact in transmission is assessed.

  16. Effective amplification of real WDM burst traffic using optical gain clamping

    OpenAIRE

    Zannin, Marcelo; Taccheo, Stefano; Ennser, Karin; Careglio, Davide; Solé Pareta, Josep

    2008-01-01

    Experimental studies of real optical burst traffic in WDM systems are performed with optical gain clamping for stabilizing the EDFA amplification. Impairments of power variation due to burst are shown to be negligible.

  17. Suppression of Spiral Waves by Voltage Clamp Techniques in a Conductance-Based Cardiac Tissue Model

    Institute of Scientific and Technical Information of China (English)

    YU Lian-Chun; MA Jun; ZHANG Guo-Yong; CHEN Yong

    2008-01-01

    A new control method is proposed to control the spatio-temporal dynamics in excitable media, which is described by the Morris-Lecar cells model. It is confirmed that successful suppression of spiral waves can be obtained by spatially clamping the membrane voltage of the excitable cells. The low voltage clamping induces breakup of spiral waves and the fragments are soon absorbed by low voltage obstacles, whereas the high voltage clamping generates travel waves that annihilate spiral waves through collision with them. However, each method has its shortcomings. Furthermore, a two-step method that combines both low and high voltage clamp techniques is then presented as a possible way of out this predicament.

  18. A novel voltage clamp technique for mapping ionic currents from cultured skeletal myotubes.

    OpenAIRE

    Anson, B D; Roberts, W M

    1998-01-01

    The biophysical properties and cellular distribution of ion channels largely determine the input/output relationships of electrically excitable cells. A variety of patch pipette voltage clamp techniques are available to characterize ionic currents. However, when used by themselves, such techniques are not well suited to the task of mapping low-density channel distributions. We describe here a new voltage clamp method (the whole cell loose patch (WCLP) method) that combines whole-cell recordin...

  19. Delayed umbilical cord clamping after childbirth: potential benefits to baby's health

    Directory of Open Access Journals (Sweden)

    Uwins C

    2014-11-01

    Full Text Available Christina Uwins,1 David JR Hutchon2 1Department of Obstetrics and Gynaecology, Croydon University Hospital, Croydon, 2Department of Obstetrics, Darlington Memorial Hospital, Darlington, UKAbstract: Early cord clamping was initially introduced as part of the package of care known as “active management of the third stage”, which was implemented to reduce postpartum hemorrhage. It has now been shown to provide no benefit to the mother and to result in harm to the neonate. The clinical trial evidence relating to delayed cord clamping compared to immediate cord clamping is presented and the physiological rationale for delayed cord clamping is discussed in this paper. Most organizations (eg World Health Organization (WHO, Royal College of Obstetricians and Gynaecologists (RCOG, Resuscitation Council (UK,The International Federation of Gynecology and Obstetrics (FIGO, International Confederation of Midwives, International Liaison Committee on Resuscitation (ILCOR and the European Resuscitation Council now advise a delay of 1–3 minutes before clamping the cord in term and preterm infants, and clinicians need to be aware of this change. Healthy neonates benefit from a more physiological and gentle transition from placental to pulmonary respiration, and we explain why this benefit should be provided to all neonates until there is any evidence to the contrary. The harm of early cord clamping is not limited to anemia and iron deficiency, and evidence for a wide range of possible harms of early cord clamping is presented. The need for resuscitation is one of the most common concerns, and ways of overcoming these concerns are described.Keywords: transition, cord clamping, hypovolemia, intraventricular hemorrhage

  20. Development of a Reusable, Low-Shock Clamp Band Separation System for Small Spacecraft Release Applications

    OpenAIRE

    Dowen, David; Christiansen, Scott; Peffer, Andrew

    2001-01-01

    In small spacecraft, the proximity of sensitive components to release systems has led to the need for lowshock spacecraft release systems. Marmon band systems are often desirable for their flight history, structural capability, and reliability. Until recently, only pyrotechnically released clamp bands were readily available. The clamp band system described in ths paper reduces shock in two ways: it eliminates shock typically associated with pyrotechnic release devices as well as utilizing a r...

  1. Patch Clamp: A Powerful Technique for Studying the Mechanism of Acupuncture

    Directory of Open Access Journals (Sweden)

    D. Zhang

    2012-01-01

    Full Text Available Cellular and molecular events can be investigated using electrophysiological techniques. In particular, the patch-clamp method provides detailed information. In addition, the patch-clamp technique has become a powerful method for investigating the mechanisms underlying the effects of acupuncture. In this paper, recent researches on how acupuncture might modulate electrophysiological responses in the central nervous system (CNS and affect peripheral structures are reviewed.

  2. Three Level Diode Clamped Inverter Fed Induction Motor with Dtc-Svm

    OpenAIRE

    V. Ranjith Kumar

    2013-01-01

    This project presents a three-level diode-clamped inverter feeding an asynchronous motor drive with direct torque control (DTC). The control method is based on DTC operating principles. The stator voltage vector reference is computed from the stator flux and torque errors imposed by the flux and torque controllers. This voltage reference is then generated using a diode-clamped inverter, where each phase of the inverter can be implemented using a dc source, which would be available from fuel c...

  3. Active Clamped ZVS Forward Converter With Soft-Switched Synchronous Rectifier

    OpenAIRE

    AÇIK, Adnan; ÇADIRCI, Işık

    2002-01-01

    This paper presents the analysis, design and implementation of an active-clamped, ZVS forward converter equipped with a soft-switched synchronous rectifier (ACFC-SR) proposed for high-efficiency, low output voltage dc-dc converter applications. The converter efficiency is maximized due to the soft-switching of the main, active clamp, synchronous rectifier and freewheeling MOSFET switches. The operating principles of the ACFC-SR are analyzed in detail and the converter performance is ...

  4. Deformation and perforation of clamped work-hardening plates struck transversely by blunt missiles

    International Nuclear Information System (INIS)

    A simple theory is given in this paper for the behaviour of clamped circular work-hardening plates struck transversely by flat-ended projectiles which travel at low velocities. By closely following the previous studies, force-displacement relationships are obtained and used to predict the deformation and perforation of the plates. It is shown that the theoretical predictions agree well with the experimental results on fully clamped steel plates when strain rate effects are taken into account. (orig.)

  5. Single-stage active clamp power factor correction AC/DC converter

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A simple single-stage AC/DC converter circuit with active clamp is presented. The operation theory and state are analyzed. The experimental results show that the voltage across main switch can be clamped to a certain value, and zero voltage switching (ZVS) can be achieved. The voltage stress and switching loss are both decreased. In range of the whole load, power factors can be always more than 97%, and the highest efficiency can reach 88%.

  6. 蒸汽驱井下作业技术研究%Steam flooding down-hole operation

    Institute of Scientific and Technical Information of China (English)

    陈东

    2016-01-01

    锦45块稠油蒸汽驱井主要存在汽窜井喷、H2S中毒、高温伤害等风险,给井下作业带来极大的难度,严重影响和制约了安全生产工作。相关人员经过探索实践,研究出了一系列井下作业技术,为蒸汽驱作业提供了经验。%Accidents, such as well blow-out caused by steam channeling, hydrogen sulfide intoxication and high temperature hazard, may occur in heavy oil steam flooding well in Jin 45 block, which bring great difficulties to down-hole operation and delay the safe operation. This article describes a series of down-hole operation technologies which are developed for steam flooding.

  7. Modeling CICR in rat ventricular myocytes: voltage clamp studies

    Directory of Open Access Journals (Sweden)

    Palade Philip T

    2010-11-01

    Full Text Available Abstract Background The past thirty-five years have seen an intense search for the molecular mechanisms underlying calcium-induced calcium-release (CICR in cardiac myocytes, with voltage clamp (VC studies being the leading tool employed. Several VC protocols including lowering of extracellular calcium to affect Ca2+ loading of the sarcoplasmic reticulum (SR, and administration of blockers caffeine and thapsigargin have been utilized to probe the phenomena surrounding SR Ca2+ release. Here, we develop a deterministic mathematical model of a rat ventricular myocyte under VC conditions, to better understand mechanisms underlying the response of an isolated cell to calcium perturbation. Motivation for the study was to pinpoint key control variables influencing CICR and examine the role of CICR in the context of a physiological control system regulating cytosolic Ca2+ concentration ([Ca2+]myo. Methods The cell model consists of an electrical-equivalent model for the cell membrane and a fluid-compartment model describing the flux of ionic species between the extracellular and several intracellular compartments (cell cytosol, SR and the dyadic coupling unit (DCU, in which resides the mechanistic basis of CICR. The DCU is described as a controller-actuator mechanism, internally stabilized by negative feedback control of the unit's two diametrically-opposed Ca2+ channels (trigger-channel and release-channel. It releases Ca2+ flux into the cyto-plasm and is in turn enclosed within a negative feedback loop involving the SERCA pump, regulating[Ca2+]myo. Results Our model reproduces measured VC data published by several laboratories, and generates graded Ca2+ release at high Ca2+ gain in a homeostatically-controlled environment where [Ca2+]myo is precisely regulated. We elucidate the importance of the DCU elements in this process, particularly the role of the ryanodine receptor in controlling SR Ca2+ release, its activation by trigger Ca2+, and its

  8. MEMS-based clamp with a passive hold function for precision position retaining of micro manipulators

    International Nuclear Information System (INIS)

    In this paper the design, modeling and fabrication of a precision MEMS-based clamp with a relatively large clamping force are presented. The purpose of the clamp is to mechanically fix a six-degree-of-freedom (DOF) MEMS-based sample manipulator (Brouwer et al J. Int. Soc. Precis. Eng. Nanotechnol. submitted) once the sample has been positioned in all DOFs. The clamping force is generated by a rotational electrostatic comb-drive actuator and can be latched passively by a parallel plate type electrostatically driven locking device. The clamp design is based on the principles of exact constraint design, resulting in a high actuation compliance (flexibility) combined with a high suspension stiffness. Therefore, a relatively large blocking force of 1.4 mN in relation to the used area of 1.8 mm2 is obtained. The fabrication is based on silicon bulk micromachining technology and combines a high-aspect-ratio deep reactive ion etching (DRIE), conformal deposition of low-pressure chemical vapor deposition (LPCVD) silicon nitride and an anisotropic potassium hydroxide (KOH) backside etching technology. Special attention is given to void reduction of SixNy trench isolation and reduction of heating phenomena during front-side release etching. Guidelines are given for the applied process. Measurements showed that the clamp was able to fix, hold and release a test actuator. The dynamic behavior was in good agreement with the modal analysis

  9. Early identification of hERG liability in drug discovery programs by automated patch clamp

    Directory of Open Access Journals (Sweden)

    Timm eDanker

    2014-09-01

    Full Text Available Blockade of the cardiac ion channel coded by hERG can lead to cardiac arrhythmia, which has become a major concern in drug discovery and development. Automated electrophysiological patch clamp allows assessment of hERG channel effects early in drug development to aid medicinal chemistry programs and has become routine in pharmaceutical companies. However, a number of potential sources of errors in setting up hERG channel assays by automated patch clamp can lead to misinterpretation of data or false effects being reported. This article describes protocols for automated electrophysiology screening of compound effects on the hERG channel current. Protocol details and the translation of criteria known from manual patch clamp experiments to automated patch clamp experiments to achieve good quality data are emphasized. Typical pitfalls and artifacts that may lead to misinterpretation of data are discussed. While this article focuses on hERG channel recordings using the QPatch (Sophion A/S, Copenhagen, Denmark technology, many of the assay and protocol details given in this article can be transferred for setting up different ion channel assays by automated patch clamp and are similar on other planar patch clamp platforms.

  10. Effect of non-ideal clamping shape on the resonance frequencies of silicon nanocantilevers

    Energy Technology Data Exchange (ETDEWEB)

    Guillon, Samuel; Saya, Daisuke; Mazenq, Laurent; Nicu, Liviu [CNRS, LAAS, 7 Avenue du Colonel Roche, F-31077 Toulouse Cedex 4 (France); Perisanu, Sorin; Vincent, Pascal [LPMCN, Universite Claude Bernard Lyon 1 et CNRS, 43 boulevard du 11 novembre 1918, 69622 Villeurbanne Cedex (France); Lazarus, Arnaud; Thomas, Olivier, E-mail: sguillon@laas.fr [Structural Mechanics and Coupled Systems Laboratory, Conservatoire National des Arts et Metiers, 2 rue Conte, 75003 Paris (France)

    2011-06-17

    In this paper, we investigate the effects of non-ideal clamping shapes on the dynamic behavior of silicon nanocantilevers. We fabricated silicon nanocantilevers using silicon on insulator (SOI) wafers by employing stepper ultraviolet (UV) lithography, which permits a resolution of under 100 nm. The nanocantilevers were driven by electrostatic force inside a scanning electron microscope (SEM). Both lateral and out-of-plane resonance frequencies were visually detected with the SEM. Next, we discuss overhanging of the cantilever support and curvature at the clamping point in the silicon nanocantilevers, which generally arises in the fabrication process. We found that the fundamental out-of-plane frequency of a realistically clamped cantilever is always lower than that for a perfectly clamped cantilever, and depends on the cantilever width and the geometry of the clamping point structure. Using simulation with the finite-elements method, we demonstrate that this discrepancy is attributed to the particular geometry of the clamping point (non-zero joining curvatures and a flexible overhanging) that is obtained in the fabrication process. The influence of the material orthotropy is also investigated and is shown to be negligible.

  11. Peptide- and proton-driven allosteric clamps catalyze anthrax toxin translocation across membranes.

    Science.gov (United States)

    Das, Debasis; Krantz, Bryan A

    2016-08-23

    Anthrax toxin is an intracellularly acting toxin in which sufficient information is available regarding the structure of its transmembrane channel, allowing for detailed investigation of models of translocation. Anthrax toxin, comprising three proteins-protective antigen (PA), lethal factor (LF), and edema factor-translocates large proteins across membranes. Here we show that the PA translocase channel has a transport function in which its catalytic active sites operate allosterically. We find that the phenylalanine clamp (ϕ-clamp), the known conductance bottleneck in the PA translocase, gates as either a more closed state or a more dilated state. Thermodynamically, the two channel states have >300-fold different binding affinities for an LF-derived peptide. The change in clamp thermodynamics requires distant α-clamp and ϕ-clamp sites. Clamp allostery and translocation are more optimal for LF peptides with uniform stereochemistry, where the least allosteric and least efficiently translocated peptide had a mixed stereochemistry. Overall, the kinetic results are in less agreement with an extended-chain Brownian ratchet model but, instead, are more consistent with an allosteric helix-compression model that is dependent also on substrate peptide coil-to-helix/helix-to-coil cooperativity. PMID:27506790

  12. A High Step-Down Interleaved Buck Converter with Active-Clamp Circuits for Wind Turbines

    Directory of Open Access Journals (Sweden)

    Chih-Lung Shen

    2012-12-01

    Full Text Available In this paper, a high step-down interleaved buck coupled-inductor converter (IBCC with active-clamp circuits for wind energy conversion has been studied. In high step-down voltage applications, an IBCC can extend duty ratio and reduce voltage stresses on active switches. In order to reduce switching losses of active switches to improve conversion efficiency, a IBCC with soft-switching techniques is usually required. Compared with passive-clamp circuits, the IBCC with active-clamp circuits have lower switching losses and minimum ringing voltage of the active switches. Thus, the proposed IBCC with active-clamp circuits for wind energy conversion can significantly increase conversion efficiency. Finally, a 240 W prototype of the proposed IBCC with active-clamp circuits was built and implemented. Experimental results have shown that efficiency can reach as high as 91%. The proposed IBCC with active-clamp circuits is presented in high step-down voltage applications to verify the performance and the feasibility for energy conversion of wind turbines.

  13. M. tuberculosis sliding β-clamp does not interact directly with the NAD+-dependent DNA ligase.

    Directory of Open Access Journals (Sweden)

    Vandna Kukshal

    Full Text Available The sliding β-clamp, an important component of the DNA replication and repair machinery, is drawing increasing attention as a therapeutic target. We report the crystal structure of the M. tuberculosis β-clamp (Mtbβ-clamp to 3.0 Å resolution. The protein crystallized in the space group C222(1 with cell-dimensions a = 72.7, b = 234.9 & c = 125.1 Å respectively. Mtbβ-clamp is a dimer, and exhibits head-to-tail association similar to other bacterial clamps. Each monomer folds into three domains with similar structures respectively and associates with its dimeric partner through 6 salt-bridges and about 21 polar interactions. Affinity experiments involving a blunt DNA duplex, primed-DNA and nicked DNA respectively show that Mtbβ-clamp binds specifically to primed DNA about 1.8 times stronger compared to the other two substrates and with an apparent K(d of 300 nM. In bacteria like E. coli, the β-clamp is known to interact with subunits of the clamp loader, NAD(+-dependent DNA ligase (LigA and other partners. We tested the interactions of the Mtbβ-clamp with MtbLigA and the γ-clamp loader subunit through radioactive gel shift assays, size exclusion chromatography, yeast-two hybrid experiments and also functionally. Intriguingly while Mtbβ-clamp interacts in vitro with the γ-clamp loader, it does not interact with MtbLigA unlike in bacteria like E. coli where it does. Modeling studies involving earlier peptide complexes reveal that the peptide-binding site is largely conserved despite lower sequence identity between bacterial clamps. Overall the results suggest that other as-yet-unidentified factors may mediate interactions between the clamp, LigA and DNA in mycobacteria.

  14. Angola Seismicity MAP

    Science.gov (United States)

    Neto, F. A. P.; Franca, G.

    2014-12-01

    The purpose of this job was to study and document the Angola natural seismicity, establishment of the first database seismic data to facilitate consultation and search for information on seismic activity in the country. The study was conducted based on query reports produced by National Institute of Meteorology and Geophysics (INAMET) 1968 to 2014 with emphasis to the work presented by Moreira (1968), that defined six seismogenic zones from macro seismic data, with highlighting is Zone of Sá da Bandeira (Lubango)-Chibemba-Oncócua-Iona. This is the most important of Angola seismic zone, covering the epicentral Quihita and Iona regions, geologically characterized by transcontinental structure tectono-magmatic activation of the Mesozoic with the installation of a wide variety of intrusive rocks of ultrabasic-alkaline composition, basic and alkaline, kimberlites and carbonatites, strongly marked by intense tectonism, presenting with several faults and fractures (locally called corredor de Lucapa). The earthquake of May 9, 1948 reached intensity VI on the Mercalli-Sieberg scale (MCS) in the locality of Quihita, and seismic active of Iona January 15, 1964, the main shock hit the grade VI-VII. Although not having significant seismicity rate can not be neglected, the other five zone are: Cassongue-Ganda-Massano de Amorim; Lola-Quilengues-Caluquembe; Gago Coutinho-zone; Cuima-Cachingues-Cambândua; The Upper Zambezi zone. We also analyzed technical reports on the seismicity of the middle Kwanza produced by Hidroproekt (GAMEK) region as well as international seismic bulletins of the International Seismological Centre (ISC), United States Geological Survey (USGS), and these data served for instrumental location of the epicenters. All compiled information made possible the creation of the First datbase of seismic data for Angola, preparing the map of seismicity with the reconfirmation of the main seismic zones defined by Moreira (1968) and the identification of a new seismic

  15. Recent CSMIP/CALTRANS Downhole Array Data and their Application in Site Specific Analysis

    Science.gov (United States)

    Haddadi, H. R.; Graizer, V.; Shakal, T.; Hipely, P.

    2002-12-01

    The California Strong Motion Instrumentation Program (CSMIP) operates 13 downhole geotechnical arrays throughout the state of California, 8 of them instrumented with the support and cooperation of California Department of Transportation (Caltrans). More than 60 low amplitude recordings from earthquakes with 2.4downhole profile of US Geological Survey and Caltrans. At the La Cienega site the shear wave velocity increases gradually from 163 m/s at the ground surface to 653 m/s at depth of 250 m. Due to vertical propagation of waves, the one dimensional wave propagation program SHAKE91 can be used to model ground motion in the layers. The earthquake ground motion at depth of 252 m is considered as the input, and the ground motion is computed at the surface, and at depths of 18 m and 100 m using SHAKE91 program. The recorded motion at the depth of 252 m consists of the two main phases: incident wave and reflected wave from the ground surface. The incident wave is used as an input ground motion in order to prevent coupling effect. Output motions are in good agreement with the actual recordings at the ground surface, 18 m and 100 m depths. The earthquake ground motion recorded at geotechnical arrays show that the incident and reflected phases of P- waves overlap in some cases (because of higher P-wave velocity the arrival time of reflected phase cannot be visually identified) and additional analysis is necessary to distinguish the arrival

  16. Gravity and Seismic Investigations of the Santo Domingo Basin, Rio Grande Rift, New Mexico

    Science.gov (United States)

    Braile, L. W.; Ferguson, J. F.; Boucher, C.; Novitsky, C. G.; O'Shea, P. M.; Daves, J.; Marzen, R.; Mendoza, K.; Rasmussen, T.; Wei, W.; Baldridge, W. S.; Biehler, S.; Claytor, J. M.; Bischoff, S. H.; Ranasinghe, N. R.; Corredor, A.

    2014-12-01

    The SAGE (Summer of Applied Geophysical Experience) program collected new gravity, seismic, electromagnetic and down-hole temperature data in 2014 in the Santo Domingo Basin and adjacent areas of the Rio Grande Rift (RGR) area of northern New Mexico. The SAGE 2014 data collection was part of an integrated geophysical study of the area initiated in 2010 and adds data and interpretations to a W to E transect of the RGR. The transect includes previous SAGE seismic refraction and CMP reflection profiles recorded in 2010 and 2011, some industry seismic reflection data, and detailed gravity observations. Seismic data consisted of a 4.8 km NW to SE profile (120 three-component stations in four overlapping deployments, 20 m station spacing, using a Vibroseis source - 20 m spacing for reflection VPs; 800 m spacing for refraction VPs) along the Borrego Canyon road with both refraction and CMP reflection coverage. About 50,000 seismograms were recorded. The surface conditions (dry unconsolidated sediments) increased surface wave energy and limited the signal-to-noise level of reflection arrivals although some wide-angle reflections with two-way times as great as 1.8 s were visible. The refraction data were modeled with first arrival travel time methods and mainly helped identify the velocity and minimum thickness of the Tertiary Santa Fe group sedimentary rocks in the Santo Domingo Basin. Interpretation of the seismic and gravity data along the transect was aided by refraction velocities, the existence of a nearby regional seismic reflection profile from industry, and lithologies and well-logs from a deep well. Gravity modeling, with significant control on depths of interfaces and densities from the seismic and drill hole data, indicates that the Santo Domingo sedimentary basin has a total depth of about 6 km.

  17. Seismic Waveguide of Metamaterials

    CERN Document Server

    Kim, Sang-Hoon

    2012-01-01

    We have developed a new method of an earthquake-resistant design to support conventional aseismic designs using acoustic metamaterials. We suggest a simple and practical method to reduce the amplitude of a seismic wave exponentially. Our device is an attenuator of a seismic wave. Constructing a cylindrical shell-type waveguide that creates a stop-band for the seismic wave, we convert the wave into an evanescent wave for some frequency range without touching the building we want to protect.

  18. Yielding of the clamped-wire system in the Ilizarov external fixator.

    Science.gov (United States)

    Watson, M A; Matthias, K J; Maffulli, N; Hukins, D W L

    2003-01-01

    This study demonstrates that the clamped-wire system used to suspend bones within an Ilizarov external fixator yields when the perpendicular load exceeds 50 N per wire. Cyclic loading was applied to tensioned wires clamped within an Ilizarov ring component, with steadily increasing load amplitude. Wires were tested at four initial tension settings. The amount of energy lost within the clamped-wire system per load cycle was calculated for every test. The results showed that there was a consistent trend to increasing non-recoverable energy loss per load cycle when peak loads exceed 50 N for all initial tension settings. A finite element (FE) model replicating the experimental conditions was performed to investigate the levels of stress within the loaded wires. The FE model analyses showed that high stresses were generated in the wires close to the clamping sites, and that the stress levels could reasonably be expected to exceed the material yield stress when loaded to about 55 N, for all initial tension settings. The results show that material yield, accompanied by some wire slippage through the clamps, is responsible for system yield, in agreement with previous studies. Although the initial wire tension has an appreciable effect on the wire stiffness, it did not affect the elastic load range of the clamped-wire system. To prevent yield of the clamped-wire system in practice, the fixator should be assembled with sufficient wires to ensure that the load transmitted to each wire by the patient does not exceed 50 N. PMID:14558649

  19. Intermittent selective clamping improves rat liver regeneration by attenuating oxidative and endoplasmic reticulum stress.

    Science.gov (United States)

    Ben Mosbah, I; Duval, H; Mbatchi, S-F; Ribault, C; Grandadam, S; Pajaud, J; Morel, F; Boudjema, K; Compagnon, P; Corlu, A

    2014-01-01

    Intermittent clamping of the portal trial is an effective method to avoid excessive blood loss during hepatic resection, but this procedure may cause ischemic damage to liver. Intermittent selective clamping of the lobes to be resected may represent a good alternative as it exposes the remnant liver only to the reperfusion stress. We compared the effect of intermittent total or selective clamping on hepatocellular injury and liver regeneration. Entire hepatic lobes or only lobes to be resected were subjected twice to 10 min of ischemia followed by 5 min of reperfusion before hepatectomy. We provided evidence that the effect of intermittent clamping can be damaging or beneficial depending to its mode of application. Although transaminase levels were similar in all groups, intermittent total clamping impaired liver regeneration and increased apoptosis. In contrast, intermittent selective clamping improved liver protein secretion and hepatocyte proliferation when compared with standard hepatectomy. This beneficial effect was linked to better adenosine-5'-triphosphate (ATP) recovery, nitric oxide production, antioxidant activities and endoplasmic reticulum adaptation leading to limit mitochondrial damage and apoptosis. Interestingly, transient and early chaperone inductions resulted in a controlled activation of the unfolded protein response concomitantly to endothelial nitric oxide synthase, extracellular signal-regulated kinase-1/2 (ERK1/2) and p38 MAPK activation that favors liver regeneration. Endoplasmic reticulum stress is a central target through which intermittent selective clamping exerts its cytoprotective effect and improves liver regeneration. This procedure could be applied as a powerful protective modality in the field of living donor liver transplantation and liver surgery. PMID:24603335

  20. Evaluation of downhole electrical heating in heavy oil of the Orinoco belt, Venezuela

    Energy Technology Data Exchange (ETDEWEB)

    Marfissi, S.; Campos, F.; Osuna, C.; Brown, J. [PDVSA Petroleos de Venezuela SA, Caracas (Venezuela, Bolivarian Republic of). Exploration and Production

    2009-07-01

    The study analyzed the benefits of using downhole electrical heating technologies at extra-heavy and heavy oil fields in Venezuela. A method of simulating artificial lift methods using a nodal analysis technique for rod pumping and progressive cavity pumps was presented. Wells were configured in relation to pump type, lengths and tubing and casing diameters. Vertical and horizontal multiphase flow correlations were used for heavy and extra-heavy oil production. The fluid characteristics of fluids, water cut, oil rates and well productivity indices from wells located in the oil fields were used in the artificial lift simulations. Current and predictive oil production rates were analyzed. Computational fluid dynamics (CFD) were conducted in order to determine the influence of the electrical heating system on the pay zone and reservoir formation. The study showed that heating generated a significant reduction in oil viscosity and reduced frictional pressure drops, which in turn allowed the pumps to handle a greater amount of fluids. Higher flow rates reduced heat losses. Oil temperatures at the pump inlet did not influence elastomer properties in progressive cavity pumps. It was concluded that productivity increases when wells are shut down for a period of 7 days during the heating process. 6 refs., 1 tab., 14 figs.

  1. Subsurface sediment contamination during borehole drilling with an air-actuated down-hole hammer

    Science.gov (United States)

    Malard, Florian; Datry, Thibault; Gibert, Janine

    2005-10-01

    Drilling methods can severely alter physical, chemical, and biological properties of aquifers, thereby influencing the reliability of water samples collected from groundwater monitoring wells. Because of their fast drilling rate, air-actuated hammers are increasingly used for the installation of groundwater monitoring wells in unconsolidated sediments. However, oil entrained in the air stream to lubricate the hammer-actuating device can contaminate subsurface sediments. Concentrations of total hydrocarbons, heavy metals (Cu, Ni, Cr, Zn, Pb, and Cd), and nutrients (particulate organic carbon, nitrogen, and phosphorus) were measured in continuous sediment cores recovered during the completion of a 26-m deep borehole drilled with a down-hole hammer in glaciofluvial deposits. Total hydrocarbons, Cu, Ni, Cr and particulate organic carbon (POC) were all measured at concentrations far exceeding background levels in most sediment cores. Hydrocarbon concentration averaged 124 ± 118 mg kg - 1 dry sediment ( n = 78 samples) with peaks at depths of 8, 14, and 20 m below the soil surface (maximum concentration: 606 mg kg - 1 ). The concentrations of hydrocarbons, Cu, Ni, Cr, and POC were positively correlated and exhibited a highly irregular vertical pattern, that probably reflected variations in air loss within glaciofluvial deposits during drilling. Because the penetration of contaminated air into the formation is unpreventable, the representativeness of groundwater samples collected may be questioned. It is concluded that air percussion drilling has strong limitations for well installation in groundwater quality monitoring surveys.

  2. Applications of TMD pulsed neutron logs in unusual downhole logging environments

    International Nuclear Information System (INIS)

    The Welex Thermal Multigate Decay logging system utilizes a pulsed 14 MeV neutron generator and two gamma ray detectors to obtain measurements of the capture cross sections of downhole formations. The composite decay curve from both formation and borehole capture ganna rays is detected, and is separated into the two individual components. The paper begins with a brief review of the TMD logging system with emphasis on the physical significance of all the quality control and new support curves. The bulk of the paper is then placed on log examples in unusual borehole conditions: logs run across intervals where borehole conditions change; logs run in air filled boreholes; logs run in badly washed out boreholes; logs run through stuck drill pipe/collars; logs run in flowing wells. The supplemental TMD curves in these situations identify the presence of gravel packs and packers, changes in borehole fluid salinity, possible communication between zones, and water and oil producing perforations. Gas in the borehole can also be differentiated from gas in the formation

  3. Clampe de Ganz no tratamento de urgência em lesões do anel pélvico Ganz clamps to treat emergencies in pelvic ring lesions

    Directory of Open Access Journals (Sweden)

    Gilberto José Cação Pereira

    2008-07-01

    Full Text Available OBJETIVO: Avaliar a eficiência do clampe de Ganz na estabilização e redução dos deslocamentos da pelve, quando utilizado no tratamento de urgência, além de aquilatar eventuais dificuldades e facilidades do método. MÉTODOS: O clampe de Ganz foi utilizado no tratamento de urgência em 31 pacientes com graves lesões do anel pélvico (Tile C associadas a importante instabilidade hemodinâmica. RESULTADO: Entre os pacientes, 27 (87,1% apresentaram evolução favorável, com estabilização, redução dos deslocamentos e compressão da região posterior do anel, além de estabilização hemodinâmica, e quatro (12,9% evoluíram para óbito. CONCLUSÃO: O clampe de Ganz mostrou-se eficiente ferramenta no tratamento de urgência das lesões do anel pélvico, por ser de concepção simples, de rápida colocação, não impedir ou dificultar procedimentos no abdome e, principalmente, por permitir a estabilização da pelve, redução dos deslocamentos e compressão na região posterior do anel, local onde ocorrem os maiores sangramentos, reduzindo-os ou eliminando-os.OBJECTIVE: To evaluate the effectiveness of Ganz clamp to stabilize and reduce pelvic dislocations in emergency situations, and to assess possible difficult and easy aspects of the method. METHOD: Ganz clamp was used in the emergency treatment of 31 patients with severe lesions of the pelvic ring (Tile C associated to major hemodynamic instability. RESULTS: Among the patients, 27 (87.1% presented a favorable evolution, with stabilization, reduction of dislocations, and compression of the posterior portion of the ring, and also hemodynamic stabilization, and 4 (12.9% died. CONCLUSION: The Ganz clamp was found to be an effective tool in the emergency treatment of pelvic ring lesions because it has an uncomplicated conception, allowing for a fast placement, because it does not prevent nor make it difficult for abdominal procedures, and most of all because it allows for the

  4. Burar seismic station: evaluation of seismic performance

    International Nuclear Information System (INIS)

    A new seismic monitoring system, the Bucovina Seismic Array (BURAR), has been established since July 2002, in the Northern part of Romania, in a joint effort of the Air Force Technical Applications Center, USA, and the National Institute for Earth Physics (NIEP), Romania. The small-aperture array consists of 10 seismic sensors (9 vertical short-period and one three-component broad band) located in boreholes and distributed in a 5 x 5 km2 area. At present, the seismic data are continuously recorded by the BURAR and transmitted in real-time to the Romanian National Data Center in Bucharest and National Data Center of the USA, in Florida. Based on the BURAR seismic information gathered at the National Data Center, NIEP (ROMNDC), in the August 2002 - December 2004 time interval, analysis and statistical assessments were performed. Following the preliminary processing of the data, several observations on the global performance of the BURAR system were emphasized. Data investigation showed an excellent efficiency of the BURAR system particularly in detecting teleseismic and regional events. Also, a statistical analysis for the BURAR detection capability of the local Vrancea events was performed in terms of depth and magnitude for the year 2004. The high signal detection capability of the BURAR resulted, generally, in improving the location solutions for the Vrancea seismic events. The location solution accuracy is enhanced when adding BURAR recordings, especially in the case of low magnitude events (recorded by few stations). The location accuracy is increased, both in terms of constraining hypocenter depth and epicentral coordinates. Our analysis certifies the importance of the BURAR system in NIEP efforts to elaborate seismic bulletins. Furthermore, the specific procedures for array data processing (beam forming, f-k analysis) increase significantly the signal-to-noise ratio by summing up the coherent signals from the array components, and ensure a better accuracy of

  5. A bacterial toxin inhibits DNA replication elongation through a direct interaction with the β sliding clamp.

    Science.gov (United States)

    Aakre, Christopher D; Phung, Tuyen N; Huang, David; Laub, Michael T

    2013-12-12

    Toxin-antitoxin (TA) systems are ubiquitous on bacterial chromosomes, yet the mechanisms regulating their activity and the molecular targets of toxins remain incompletely defined. Here, we identify SocAB, an atypical TA system in Caulobacter crescentus. Unlike canonical TA systems, the toxin SocB is unstable and constitutively degraded by the protease ClpXP; this degradation requires the antitoxin, SocA, as a proteolytic adaptor. We find that the toxin, SocB, blocks replication elongation through an interaction with the sliding clamp, driving replication fork collapse. Mutations that suppress SocB toxicity map to either the hydrophobic cleft on the clamp that binds DNA polymerase III or a clamp-binding motif in SocB. Our findings suggest that SocB disrupts replication by outcompeting other clamp-binding proteins. Collectively, our results expand the diversity of mechanisms employed by TA systems to regulate toxin activity and inhibit bacterial growth, and they suggest that inhibiting clamp function may be a generalizable antibacterial strategy. PMID:24239291

  6. Dynamics of beta and proliferating cell nuclear antigen sliding clamps in traversing DNA secondary structure.

    Science.gov (United States)

    Yao, N; Hurwitz, J; O'Donnell, M

    2000-01-14

    Chromosomal replicases of cellular organisms utilize a ring shaped protein that encircles DNA as a mobile tether for high processivity in DNA synthesis. These "sliding clamps" have sufficiently large linear diameters to encircle duplex DNA and are perhaps even large enough to slide over certain DNA secondary structural elements. This report examines the Escherichia coli beta and human proliferating cell nuclear antigen clamps for their ability to slide over various DNA secondary structures. The results show that these clamps are capable of traversing a 13-nucleotide ssDNA loop, a 4-base pair stem-loop, a 4-nucleotide 5' tail, and a 15-mer bubble within the duplex. However, upon increasing the size of these structures (20-nucleotide loop, 12-base pair stem-loop, 28-nucleotide 5' tail, and 20-nucleotide bubble) the sliding motion of the beta and proliferating cell nuclear antigen over these elements is halted. Studies of the E. coli replicase, DNA polymerase III holoenzyme, in chain elongation with the beta clamp demonstrate that upon encounter with an oligonucleotide annealed in its path, it traverses the duplex and resumes synthesis on the 3' terminus of the oligonucleotide. This sliding and resumption of synthesis occurs even when the oligonucleotide contains a secondary structure element, provided the beta clamp can traverse the structure. However, upon encounter with a downstream oligonucleotide containing a large internal secondary structure, the holoenzyme clears the obstacle by strand displacing the oligonucleotide from the template. Implications of these protein dynamics to DNA transactions are discussed. PMID:10625694

  7. Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience

    Directory of Open Access Journals (Sweden)

    Yi Zhou

    2015-05-01

    Full Text Available Neuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal activity, such as spiking responses or membrane potential dynamics, but also quantify synaptic inputs from excitatory and inhibitory circuits in living animals. This approach enables researchers to directly unravel different synaptic components and to understand their underlying roles in particular brain functions. Combining in vivo patch-clamp recording with other techniques, such as two-photon imaging or optogenetics, can provide even clearer functional dissection of the synaptic contributions of different neurons or nuclei. Here, we summarized current applications and recent research progress using the in vivo patch-clamp recording method and focused on its role in the functional dissection of different synaptic inputs. The key factors of a successful in vivo patch-clamp experiment and possible solutions based on references and our experiences were also discussed.

  8. Gain-clamping techniques in two-stage double-pass L-band EDFA

    Indian Academy of Sciences (India)

    S W harun; N Md Samsuri; H Ahmad

    2006-03-01

    Two designs of long-wavelength band erbium-doped fiber amplifier (L-band EDFA) for gain clamping in double-pass systems are demonstrated and compared. The first design is based on ring laser technique where a backward amplified spontaneous emission (ASE) from the second stage is routed into the feedback loop to create an oscillating laser for gain clamping. The gain is clamped at 18.6 dB from -40 to -8 dBm with a gain variation of less than ± 0.1 dB and a noise figure of less than 6 dB. Another scheme is based on partial reflection of ASE into the EDFA, which is demonstrated using a narrowband fiber Bragg grating. This scheme achieves a good gain clamping characteristic up to -12 dBm of input signal power with a gain variation of less than ± 0.3 dB from a clamped gain of 22 dB. The noise figure of a 1580 nm signal is maintained below 5 dB in this amplifier since this scheme is not based on lasing mechanism. The latter scheme is also expected to be free from the relaxation oscillation problem.

  9. Nonlinear Bending Stiffness of Plates Clamped by Bolted Joints under Bending Moment

    Science.gov (United States)

    Naruse, Tomohiro; Shibutani, Yoji

    Equivalent stiffness of plates clamped by bolted joints for designing should be evaluated according to not only the strength of bolted joints but also the deformation and vibration characteristics of the structures. When the applied external axial load or the bending moment is sufficiently small, the contact surfaces of the bolted joint are stuck together, and thus both the bolt and the clamped plates deform linearly. Although the sophisticated VDI 2230 code gives the appropriate stiffness of clamped plates for the infinitesimal deformation, the stiffness may vary nonlinearly with increasing the loading because of changing the contact state. Therefore, the present paper focuses on the nonlinear behaviour of the bending stiffness of clamped plates by using Finite Element (FE) analyses, taking the contact condition on bearing surfaces and between the plates into account. The FE models of the plates with thicknesses of 3.2, 4.5, 6.0 and 9.0 mm tightened with M8, 10, 12 and 16 bolts were constructed. The relation between bending moment and bending compliance of clamped plates is found to be categorized into three regions, namely, (i) constant compliance with fully stuck contact surfaces, (ii) transition showing the nonlinear compliance, and (iii) constant compliance with one-side contact surfaces. The mechanical models for these three regions are proposed and compared with FEM solutions. The prediction on the bounds of three regions is in a fairly good agreement except the case with smaller bolts and thicker plates.

  10. Geophysical Monitoring at the CO2SINK Site: Combining Seismic and Geoelectric Data

    Science.gov (United States)

    Giese, R.; Lüth, S.; Cosma, C.; Juhlin, C.; Kiessling, D.; Schütt, H.; Schöbel, B.; Schmidt-Hattenberger, C.; Schilling, F.; Co2SINK Group

    2009-04-01

    The CO2SINK project at the German town of Ketzin (near Berlin), is aimed at a pilot storage of CO2, and at developing and testing efficient integrated monitoring procedures (physical, chemical, and biological observations) for assessing the processes triggered within the reservoir by a long term injection operation. In particular, geophysical methods as seismic and geoelectric measurements have delivered the structural framework, and they enable to observe the reaction of the reservoir and the caprock to CO2 propagation at locations which are not accessible for direct observations. We report on the seismic monitoring program of the CO2SINK project which comprises baseline and repeat observations at different scales in time and space, combined with comprehensive geoelectrical monitoring performed in the Ketzin wells and on the surface. The main objectives of the 3D seismic survey (carried out in spring 2005) were to provide the structural model around the location of the Ketzin wells, to verify earlier geologic interpretations of structure based on vintage 2D seismic and borehole data, as well as providing a baseline for future seismic surveys. The uppermost 1000 m are well imaged and show an anticlinal structure with an east-west striking central graben on its top. The 3D baseline survey was extended by VSP (vertical seismic profiling), MSP (moving source profiling) on 7 profiles, and crosshole tomographic measurements. 2D "star" measurements were carried out on the 7 MSP profiles in order to tie-in the down-hole surveys with the 3D baseline survey. These measurements provide enhanced resolution in time (faster and more cost effective than a full 3D survey) and space (higher source and receiver frequencies). Three crosshole measurements were performed, one baseline survey in May 2008, and two repeats in July and August 2008, respectively. A third crosshole repeat is planned for a later stage in the project when a steady state situation has been reached in the

  11. Combined Triplex/Duplex Invasion of Double-Stranded DNA by "Tail-Clamp" Peptide Nucleic Acid

    DEFF Research Database (Denmark)

    Bentin, Thomas; Larsen, H. J.; Nielsen, Peter E.

    2003-01-01

    "Tail-clamp" PNAs composed of a short (hexamer) homopyrimidine triplex forming domain and a (decamer) mixed sequence duplex forming extension have been designed. Tail-clamp PNAs display significantly increased binding to single-stranded DNA compared with PNAs lacking a duplex-forming extension as...... determined by T-m measurements. Binding to double-stranded (ds) DNA occurred by combined triplex and duplex invasion as analyzed by permanganate probing. Furthermore, C-50 measurements revealed that tail-clamp PNAs consistently bound the dsDNA target more efficiently, and kinetics experiments revealed that...... this was due to a dramatically reduced dissociation rate of such complexes. Increasing the PNA net charge also increased binding efficiency, but unexpectedly, this increase was much more pronounced for tailless-clamp PNAs than for tail-clamp PNAs. Finally, shortening the tail-clamp PNA triplex invasion...

  12. Integrated analysis of well logs and seismic data to estimate gas hydrate concentrations at Keathley Canyon, Gulf of Mexico

    Science.gov (United States)

    Lee, M.W.; Collett, T.S.

    2008-01-01

    Accurately detecting and quantifying gas hydrate or free gas in sediments from seismic data require downhole well-log data to calibrate the physical properties of the gas hydrate-/free gas-bearing sediments. As part of the Gulf of Mexico Joint Industry Program, a series of wells were either cored or drilled in the Gulf of Mexico to characterize the physical properties of gas hydrate-bearing sediments, to calibrate geophysical estimates, and to evaluate source and transport mechanisms for gas within the gas hydrates. Downhole acoustic logs were used sparingly in this study because of degraded log quality due to adverse wellbore conditions. However, reliable logging while drilling (LWD) electrical resistivity and porosity logs were obtained. To tie the well-log information to the available 3-D seismic data in this area, a velocity log was calculated from the available resistivity log at the Keathley Canyon 151-2 well, because the acoustic log or vertical seismic data acquired at the nearby Keathley Canyon 151-3 well were either of poor quality or had limited depth coverage. Based on the gas hydrate saturations estimated from the LWD resistivity log, the modified Biot-Gassmann theory was used to generate synthetic acoustic log and a synthetic seismogram was generated with a fairly good agreement with a seismic profile crossing the well site. Based on the well-log information, a faintly defined bottom-simulating reflection (BSR) in this area is interpreted as a reflection representing gas hydrate-bearing sediments with about 15% saturation overlying partially gas-saturated sediments with 3% saturation. Gas hydrate saturations over 30-40% are estimated from the resistivity log in two distinct intervals at 220-230 and 264-300 m below the sea floor, but gas hydrate was not physically recovered in cores. It is speculated that the poor recovery of cores and gas hydrate morphology are responsible for the lack of physical gas hydrate recovery.

  13. Surface-downhole and crosshole geoelectrics for monitoring of brine injection at the Ketzin CO2 storage site

    Science.gov (United States)

    Rippe, Dennis; Bergmann, Peter; Labitzke, Tim; Wagner, Florian; Schmidt-Hattenberger, Cornelia

    2016-04-01

    The Ketzin pilot site in Germany is the longest operating on-shore CO2 storage site in Europe. From June 2008 till August 2013, a total of ˜67,000 tonnes of CO2 were safely stored in a saline aquifer at depths of 630 m to 650 m. The storage site has now entered the abandonment phase, and continuation of the multi-disciplinary monitoring as part of the national project "CO2 post-injection monitoring and post-closure phase at the Ketzin pilot site" (COMPLETE) provides the unique chance to participate in the conclusion of the complete life cycle of a CO2 storage site. As part of the continuous evaluation of the functionality and integrity of the CO2 storage in Ketzin, from October 12, 2015 till January 6, 2015 a total of ˜2,900 tonnes of brine were successfully injected into the CO2 reservoir, hereby simulating in time-lapse the natural backflow of brine and the associated displacement of CO2. The main objectives of this brine injection experiment include investigation of how much of the CO2 in the pore space can be displaced by brine and if this displacement of CO2 during the brine injection differs from the displacement of formation fluid during the initial CO2 injection. Geophysical monitoring of the brine injection included continuous geoelectric measurements accompanied by monitoring of pressure and temperature conditions in the injection well and two adjacent observation wells. During the previous CO2 injection, the geoelectrical monitoring concept at the Ketzin pilot site consisted of permanent crosshole measurements and non-permanent large-scale surveys (Kiessling et al., 2010). Time-lapse geoelectrical tomographies derived from the weekly crosshole data at near-wellbore scale complemented by six surface-downhole surveys at a scale of 1.5 km showed a noticeable resistivity signature within the target storage zone, which was attributed to the CO2 plume (Schmidt-Hattenberger et al., 2011) and interpreted in terms of relative CO2 and brine saturations (Bergmann

  14. Surface-downhole and crosshole geoelectrics for monitoring of brine injection at the Ketzin CO2 storage site

    Science.gov (United States)

    Rippe, Dennis; Bergmann, Peter; Labitzke, Tim; Wagner, Florian; Schmidt-Hattenberger, Cornelia

    2016-04-01

    The Ketzin pilot site in Germany is the longest operating on-shore CO2 storage site in Europe. From June 2008 till August 2013, a total of ˜67,000 tonnes of CO2 were safely stored in a saline aquifer at depths of 630 m to 650 m. The storage site has now entered the abandonment phase, and continuation of the multi-disciplinary monitoring as part of the national project "CO2 post-injection monitoring and post-closure phase at the Ketzin pilot site" (COMPLETE) provides the unique chance to participate in the conclusion of the complete life cycle of a CO2 storage site. As part of the continuous evaluation of the functionality and integrity of the CO2 storage in Ketzin, from October 12, 2015 till January 6, 2015 a total of ˜2,900 tonnes of brine were successfully injected into the CO2 reservoir, hereby simulating in time-lapse the natural backflow of brine and the associated displacement of CO2. The main objectives of this brine injection experiment include investigation of how much of the CO2 in the pore space can be displaced by brine and if this displacement of CO2 during the brine injection differs from the displacement of formation fluid during the initial CO2 injection. Geophysical monitoring of the brine injection included continuous geoelectric measurements accompanied by monitoring of pressure and temperature conditions in the injection well and two adjacent observation wells. During the previous CO2 injection, the geoelectrical monitoring concept at the Ketzin pilot site consisted of permanent crosshole measurements and non-permanent large-scale surveys (Kiessling et al., 2010). Time-lapse geoelectrical tomographies derived from the weekly crosshole data at near-wellbore scale complemented by six surface-downhole surveys at a scale of 1.5 km showed a noticeable resistivity signature within the target storage zone, which was attributed to the CO2 plume (Schmidt-Hattenberger et al., 2011) and interpreted in terms of relative CO2 and brine saturations (Bergmann

  15. Feasibility of jointly locating microseismic events with data from surface and downhole receivers

    Czech Academy of Sciences Publication Activity Database

    Jánský, J.; Plicka, V.; Eisner, Leo

    2013-01-01

    Roč. 31, č. 7 (2013), s. 65-71. ISSN 0263-5046 R&D Projects: GA MŠk LM2010008 Institutional support: RVO:67985891 Keywords : induced seismicity * migration * location Subject RIV: DC - Siesmology, Volcanology, Earth Structure http://earthdoc.eage.org/publication/publicationdetails/?publication=70358

  16. Delayed cord clamping in red blood cell alloimmunization: safe, effective, and free?

    Science.gov (United States)

    McAdams, Ryan M

    2016-04-01

    Hemolytic disease of the newborn (HDN), an alloimmune disorder due to maternal and fetal blood type incompatibility, is associated with fetal and neonatal complications related to red blood cell (RBC) hemolysis. After delivery, without placental clearance, neonatal hyperbilirubinemia may develop from ongoing maternal antibody-mediated RBC hemolysis. In cases refractory to intensive phototherapy treatment, exchange transfusions (ET) may be performed to prevent central nervous system damage by reducing circulating bilirubin levels and to replace antibody-coated red blood cells with antigen-negative RBCs. The risks and costs of treating HDN are significant, but appear to be decreased by delayed umbilical cord clamping at birth, a strategy that promotes placental transfusion to the newborn. Compared to immediate cord clamping (ICC), safe and beneficial short-term outcomes have been demonstrated in preterm and term neonates receiving delayed cord clamping (DCC), a practice that may potentially be effective in cases RBC alloimmunization. PMID:27186530

  17. Significance of Shrinkage Induced Clamping Pressure in Fiber-Matrix Bonding in Cementitious Composite Materials

    DEFF Research Database (Denmark)

    Stang, Henrik

    1996-01-01

    The present paper accesses the significance of shrinkage inducedclamping pressure in fiber/matrix bonding mechanisms incementitious composite materials. The paper contains a description of an experimental setup whichallows mbox{measurement} of the clamping pressure which develops on anelastic...... inhomogeneity embedded in a matrix consisting of acementitious material undergoing shrinkage during hydration(autogenous shrinkage). Furthermore, the paperpresents the analysis necessary to perform an interpretation of the experimental results and which allows for thedetermination of the clamping pressure...... used in high performance cementitious composite materials.Assuming a Coulomb type of friction on the fiber/matrix interface andusing typical values for the frictional coefficient it is shownthat the shrinkage induced clamping pressure could be one of the mostimportant factors determining the frictional...

  18. Enhancing endogenous stem cells in the newborn via delayed umbilical cord clamping

    Directory of Open Access Journals (Sweden)

    Christopher Lawton

    2015-01-01

    Full Text Available There is currently no consensus among clinicians and scientists over the appropriate or optimal timing for umbilical cord clamping. However, many clinical studies have suggested that delayed cord clamping is associated with various neonatal benefits including increased blood volume, reduced need for blood transfusion, increased cerebral oxygenation in pre-term infants, and decreased frequency of iron deficiency anemia in term infants. Human umbilical cord blood contains significant amounts of stem and progenitor cells and is currently used in the treatment of several life-threatening diseases. We propose that delayed cord clamping be encouraged as it enhances blood flow from the placenta to the neonate, which is accompanied by an increase supply of valuable stem and progenitor cells, as well as may improve blood oxygenation and increase blood volume, altogether reducing the infant′s susceptibility to both neonatal and age-related diseases.

  19. Enhancing endogenous stem cells in the newborn via delayed umbilical cord clamping.

    Science.gov (United States)

    Lawton, Christopher; Acosta, Sandra; Watson, Nate; Gonzales-Portillo, Chiara; Diamandis, Theo; Tajiri, Naoki; Kaneko, Yuji; Sanberg, Paul R; Borlongan, Cesar V

    2015-09-01

    There is currently no consensus among clinicians and scientists over the appropriate or optimal timing for umbilical cord clamping. However, many clinical studies have suggested that delayed cord clamping is associated with various neonatal benefits including increased blood volume, reduced need for blood transfusion, increased cerebral oxygenation in pre-term infants, and decreased frequency of iron deficiency anemia in term infants. Human umbilical cord blood contains significant amounts of stem and progenitor cells and is currently used in the treatment of several life-threatening diseases. We propose that delayed cord clamping be encouraged as it enhances blood flow from the placenta to the neonate, which is accompanied by an increase supply of valuable stem and progenitor cells, as well as may improve blood oxygenation and increase blood volume, altogether reducing the infant's susceptibility to both neonatal and age-related diseases. PMID:26604879

  20. Enhancing endogenous stem cells in the newbornvia delayed umbilical cord clamping

    Institute of Scientific and Technical Information of China (English)

    Christopher Lawton; Sandra Acosta; Nate Watson; Chiara Gonzales-Portillo; hTeo Diamandis; Naoki Tajiri; Yuji Kaneko; Paul R. Sanberg; Cesar V. Borlongan

    2015-01-01

    There is currently no consensus among clinicians and scientists over the appropriate or optimal timing for umbilical cord clamping. However, many clinical studies have suggested that delayed cord clamping is associated with various neonatal beneifts including increased blood volume, reduced need for blood transfusion, increased cerebral oxygenation in pre-term infants, and decreased frequency of iron deifciency anemia in term infants. Human umbilical cord blood con-tains signiifcant amounts of stem and progenitor cells and is currently used in the treatment of several life-threatening diseases. We propose that delayed cord clamping be encouraged as it en-hances blood lfow from the placenta to the neonate, which is accompanied by an increase supply of valuable stem and progenitor cells, as well as may improve blood oxygenation and increase blood volume, altogether reducing the infant’s susceptibility to both neonatal and age-related diseases.

  1. Development of planar patch clamp technology and its application in the analysis of cellular electrophysiology

    Institute of Scientific and Technical Information of China (English)

    Peihua Chen; Wei Zhang; Jun Zhou; Ping Wang; Lidan Xiao; Mo Yang

    2009-01-01

    A patch clamp chip, as a novel cell-based chip for electrophysiological recordings, has many prominent advantages such as high res-olution, accuracy, high throughput and automation. It can be used to perform multivariate and real-time measurements of cell networks in situ. Therefore, this technology will dramatically promote the research on ionic channels, neuronal networks and the application of this technology in drug screening. This paper reviews the development of planar patch clamp technology and its applications in detail. The latest progress in the research of taste cells electrophysiology and taste transduction is also presented. Finally, this paper analyzes the methodology of neural chips. Based on the current research of our laboratory, the prospective applications of a patch clamp chip in the research of taste sensation and transduction mechanisms at molecular and cellular levels are discussed.

  2. Seismic texture classification. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Vinther, R.

    1997-12-31

    The seismic texture classification method, is a seismic attribute that can both recognize the general reflectivity styles and locate variations from these. The seismic texture classification performs a statistic analysis for the seismic section (or volume) aiming at describing the reflectivity. Based on a set of reference reflectivities the seismic textures are classified. The result of the seismic texture classification is a display of seismic texture categories showing both the styles of reflectivity from the reference set and interpolations and extrapolations from these. The display is interpreted as statistical variations in the seismic data. The seismic texture classification is applied to seismic sections and volumes from the Danish North Sea representing both horizontal stratifications and salt diapers. The attribute succeeded in recognizing both general structure of successions and variations from these. Also, the seismic texture classification is not only able to display variations in prospective areas (1-7 sec. TWT) but can also be applied to deep seismic sections. The seismic texture classification is tested on a deep reflection seismic section (13-18 sec. TWT) from the Baltic Sea. Applied to this section the seismic texture classification succeeded in locating the Moho, which could not be located using conventional interpretation tools. The seismic texture classification is a seismic attribute which can display general reflectivity styles and deviations from these and enhance variations not found by conventional interpretation tools. (LN)

  3. Development and Test of a 1,000 Level 3C Fiber Optic Borehole Seismic Receiver Array Applied to Carbon Sequestration

    Energy Technology Data Exchange (ETDEWEB)

    Paulsson, Bjorn N.P. [Paulsson, Inc., Van Nuys, CA (United States)

    2015-02-28

    To address the critical site characterization and monitoring needs for CCS programs, US Department of Energy (DOE) awarded Paulsson, Inc. in 2010 a contract to design, build and test a fiber optic based ultra-large bandwidth clamped borehole seismic vector array capable of deploying up to one thousand 3C sensor pods suitable for deployment into high temperature and high pressure boreholes. Paulsson, Inc. has completed a design or a unique borehole seismic system consisting of a novel drill pipe based deployment system that includes a hydraulic clamping mechanism for the sensor pods, a new sensor pod design and most important – a unique fiber optic seismic vector sensor with technical specifications and capabilities that far exceed the state of the art seismic sensor technologies. These novel technologies were all applied to the new borehole seismic system. In combination these technologies will allow for the deployment of up to 1,000 3C sensor pods in vertical, deviated or horizontal wells. Laboratory tests of the fiber optic seismic vector sensors developed during this project have shown that the new borehole seismic sensor technology is capable of generating outstanding high vector fidelity data with extremely large bandwidth: 0.01 – 6,000 Hz. Field tests have shown that the system can record events at magnitudes much smaller than M-2.3 at frequencies up to 2,000 Hz. The sensors have also proved to be about 100 times more sensitive than the regular coil geophones that are used in borehole seismic systems today. The fiber optic seismic sensors have furthermore been qualified to operate at temperatures over 300°C (572°F). The fibers used for the seismic sensors in the system are used to record Distributed Temperature Sensor (DTS) data allowing additional value added data to be recorded simultaneously with the seismic vector sensor data.

  4. Delayed cord clamping for prevention of iron deficiency anemia in term infants

    Directory of Open Access Journals (Sweden)

    Olga Rasiyanti Siregar

    2012-07-01

    Full Text Available Background Iron deficiency anemia (IDA during infancy and childhood is a concern due to its potentially detrimental effects on development, some of which may be irreversible even after iron treatment. Delayed cord clamping may prevent IDA by increasing an infant’s iron reserve at birth. Objective We aimed to evaluate the effect of delayed umbilical cord clamping at birth on the iron status in newborns at age 24 hours of life. Methods This randomized, single-blind study was conducted from March to May 2009, at two general hospitals in Medan, North Sumatera Province. Eligible newborn infants were randomly assigned to one of two groups: early cord clamping (ECC performed 15 seconds after delivery or delayed cord clamping (DCC performed 2 minutes after delivery. Infants were placed on their mothers’ abdomens before the umbilical cords were clamped. Hematologic status was determined from umbilical cord blood. Results Sixty-three subjects were included in our study, consisting of 31 infants in the ECC group and 32 infants in the DCC group. We found that mean neonatal hemoglobin level was higher in the DCC group than in the ECC group (18.4 g% and 16.2 g%, respectively, P=0.0001. Also, mean ferritin level was higher in the DCC group than in the ECC group (556 mg/dL and 329 mg/dL, respectively, P=0.015. Other hematological status indicators, including mean hematocrit and mean corpuscular volume (MCV level, were also higher in the DCC group. However, mean red blood cell levels were not significantly different between the two groups. Nor was there a significant difference in mean bilirubin level between the DCC and ECC groups. Conclusion Delayed cord clamping may improve iron status and prevent IDA in term infants.

  5. Role of the α Clamp in the Protein Translocation Mechanism of Anthrax Toxin.

    Science.gov (United States)

    Brown, Michael J; Thoren, Katie L; Krantz, Bryan A

    2015-10-01

    Membrane-embedded molecular machines are utilized to move water-soluble proteins across these barriers. Anthrax toxin forms one such machine through the self-assembly of its three component proteins--protective antigen (PA), lethal factor, and edema factor. Upon endocytosis into host cells, acidification of the endosome induces PA to form a membrane-inserted channel, which unfolds lethal factor and edema factor and translocates them into the host cytosol. Translocation is driven by the proton motive force, composed of the chemical potential, the proton gradient (ΔpH), and the membrane potential (Δψ). A crystal structure of the lethal toxin core complex revealed an "α clamp" structure that binds to substrate helices nonspecifically. Here, we test the hypothesis that, through the recognition of unfolding helical structure, the α clamp can accelerate the rate of translocation. We produced a synthetic PA mutant in which an α helix was crosslinked into the α clamp to block its function. This synthetic construct impairs translocation by raising a yet uncharacterized translocation barrier shown to be much less force dependent than the known unfolding barrier. We also report that the α clamp more stably binds substrates that can form helices than those, such as polyproline, that cannot. Hence, the α clamp recognizes substrates by a general shape-complementarity mechanism. Substrates that are incapable of forming compact secondary structure (due to the introduction of a polyproline track) are severely deficient for translocation. Therefore, the α clamp and its recognition of helical structure in the translocating substrate play key roles in the molecular mechanism of protein translocation. PMID:26344833

  6. Analysis and testing of the DIII-D ohmic heating coil lead repair clamp

    International Nuclear Information System (INIS)

    DIII-D has been operating for the last year with limited volt-second capabilities due to structural failure of a conductor lead to one of the ohmic heating (OH) solenoids. The conductor failure was due to poor epoxy impregnation of the overwrap of the lead pack, resulting in copper fatigue and a water leak. A number of structural analyses were performed to assist in determining the failure scenario and to evaluate various repair options. A fatigue stress analysis of the leads with a failed epoxy overwrap indicated crack initiation after 1,000 cycles at the maximum operating conditions. The failure occurred in a very inaccessible area which restricted design repair options to concepts which could be implemented remotely. Several design options were considered for repairing the lead so that it can sustain the loads for 7.5 Vs conditions at full toroidal field. A clamp, along with preloaded banding straps and shim bags, provides a system that guarantees that the stress at the crack location is always compressive and prevents further crack growth in the conductor. Due to the limited space available for the repair, it was necessary to design the clamp system to operate at the material yield stress. The primary components of the clamp system were verified by load tests prior to installation. The main body of the clamp contains a load cell and potentiometer for monitoring the load-deflection characteristics of the clamp and conductors during plasma operation. Strain gages provides redundant instrumentation. If required, the preload on the conductors can be increased remotely by a special wrench attached to the clamp assembly

  7. IMPROVED DOWN-HOLE CPT TOOLS FOR THE DETECTION OF CHLORINATED SOLVENTS; FINAL

    International Nuclear Information System (INIS)

    Conventional soil and groundwater sampling procedures present many opportunities for loss of volatile organic compounds (VOC) by exposing sample media to the atmosphere during transfers between sampling devices and containers, ultimately affecting the quality of the analytical results. Inaccurate characterization data often leads to improperly designed remedial systems that slow the clean-up process and increase the cost. For these reasons, in situ methods for sample extraction and real time analysis provide attractive alternatives to conventional sampling and analysis. Under funding from the U.S. Department of Energy's Federal Energy Technology Center (FETC), the investigators continued development of a system that combines High Speed Gas Chromatography techniques with Cone Penetration Testing to achieve near-real time analysis of soils and groundwater for chlorinated and aromatic hydrocarbon contamination (PCE, TCE, BTEX) during site characterization. The system combines three new CPT tools with an up-hole analyzer. The Dynamic Thermal Desorption (DTD) probe provides the up-hole analyzer with continuously sampled soil gas, enhancing detection limits by heating the soil matrix during penetration to thermally desorb volatile organic contaminants. The CPT In Situ Purge Probe (CISP) purges a water sample in situ, transferring the purge gas up-hole for analysis. Alternatively, soil gas or purge gas from the DTD probe or the CISP can be diverted to a Downhole Trap Module (DTM), which traps contaminants on conventional trap media for later analysis in a fixed laboratory. While the effectiveness of the CISP was hampered by the tendency of fine grained materials to clog its sample inlet filter, the DTD probe and the DTM were demonstrated to perform their intended functions well

  8. Supervised classification of down-hole physical properties measurements using neural network to predict the lithology

    Science.gov (United States)

    Mahmoodi, Omid; Smith, Richard S.; Tinkham, Douglas K.

    2016-01-01

    The reliability of rock-type prediction using down-hole density, gamma ray response, and magnetic susceptibility measurements was evaluated at the Victoria property, Sudbury, ON. A supervised neural network, trained using lithological information from drill hole FNX1168, yields a predictive accuracy of 83% for the training data. Applying the trained network on drill hole FNX1182 resulted in 64% of the rock types being correctly classified when compared with the classification produced by geologists during logging of the core. The homogenous rock types, like quartz diorite, had a high accuracy of classification; while the heterogeneous rock types such as diabase were poorly classified. Overlap between physical properties of rock types caused by heterogeneity or inherent similarity in physical properties of rock types, which were verified by observing the cores, accounts for most of the misclassification. To reduce the misclassification, the network was trained from physical log units in FNX1168 derived from clustering of physical properties measurements. Four physical log units mainly represented four groups of rocks: i) quartz diorite; ii) metabasalt and metagabbro; iii) metasediment and quartzite; and iv) sulfide and diabase. The predictive accuracy in the training process rose to 95%. The trained network then was applied to predicting the physical log units in FNX1182. Given the relationships between physical log units and rock types from FNX1168, the results of physical-log-unit classification in FNX1182 were interpreted from a geological point of view. Although in ideal cases we would like to be able to extract the same classification that a geologist provides, the extraction of physical log units is a more realistic goal. The interpretation of the lithological units from the physical log units can be compared with the geologist's classification and discrepancies or anomalies analyzed in greater detail.

  9. Cracking associated with micrometeoroid impact craters in anodized aluminum alloy clamps on LDEF

    Science.gov (United States)

    Murr, Lawrence E.; Niou, Chorng S.; Quinones, Stella; Murr, Kyle S.

    1992-01-01

    The Long Duration Exposure Facility (LDEF) is a reusable hollow-cylindrical satellite sustaining a total of 57 different experiments. The 130 sq m of spacecraft surface area included anodized 6061-T6 Al alloy bay frames and clamps for holding experiment trays in the bay areas. Attention is presently given to the micrometeoroid impact crater features observed on two tray clamps recovered from the LDEF leading-edge locations. It is found that even very subtle surface modifications in structural alloy anodizing can influence micrometeoroid impact crater cracking, notable radial cracking due to the ejecta-rim of the impact craters.

  10. High pressure clamp for electrical measurements up to 8 GPa and temperature down to 77 K

    Science.gov (United States)

    Bandyopadhyay, A. K.; Nalini, A. V.; Gopal, E. S. R.; Subramanyam, S. V.

    1980-01-01

    A compact clamp-type high pressure cell for carrying out electrical conductivity measurements on small solid samples of size 1 mm or less at pressures upto 8 GPa (i.e., 80 kbar) and for use down to 77 K has been designed and fabricated. The pressure generated in the sample region has been calibrated at room temperature against the polymorphic phase transitions of Bismuth and Ytterbium. The pressure relaxation of the clamp at low temperatures has been estimated by monitoring the electrical conductivity behavior of lead.

  11. In vivo patch clamp recording technique in the study of neurophysiology

    Institute of Scientific and Technical Information of China (English)

    Lan-Wei CUI; Yu-Rong LI

    2006-01-01

    The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaesthesia ( or awake) animals. This technique can be used to study the synaptic function and plasticity in central nervous system in vivoin order to understand the physiological properties of the ion channels from an integrated point of view. The advantage of this technique have already presented itself in the study of the synaptic transmission and nervous network. Nowadays, in vivo patch whole-cell recording technique in combination with other techniques is becoming a common method in the research fields.

  12. Geology of the Waste Treatment Plant Seismic Boreholes

    Energy Technology Data Exchange (ETDEWEB)

    Barnett, D. Brent; Fecht, Karl R.; Reidel, Stephen P.; Bjornstad, Bruce N.; Lanigan, David C.; Rust, Colleen F.

    2007-05-11

    In 2006, the U.S. Department of Energy initiated the Seismic Boreholes Project (SBP) to emplace boreholes at the Waste Treatment Plant (WTP) site in order to obtain direct shear wave velocity (Vs) measurements and other physical property measurements in Columbia River basalt and interbedded sediments of the Ellensburg Formation. The goal was to reduce the uncertainty in the response spectra and seismic design basis, and potentially recover design margin for the WTP. The characterization effort within the deep boreholes included 1) downhole measurements of the velocity properties of the suprabasalt, basalt, and sedimentary interbed sequences, 2) downhole measurements of the density of the subsurface basalt and sediments, and 3) geologic studies to confirm the geometry of the contact between the various basalt and interbedded sediments through examination of retrieved core from the core hole and data collected through geophysical logging of each borehole. This report describes the results of the geologic studies from three mud-rotary boreholes and one cored borehole at the WTP. All four boreholes penetrated the entire Saddle Mountains Basalt and the upper part of the Wanapum Basalt where thick sedimentary interbeds occur between the lava flows. The basalt flows penetrated in Saddle Mountains Basalt included the Umatilla Member, Esquatzel Member, Pomona Member, and the Elephant Mountain Member. The underlying Priest Rapids Member of the Wanapum Basalt also was penetrated. The Ellensburg Formation sediments consist of the Mabton Interbed, the Cold Creek Interbed, the Selah Interbed, and the Rattlesnake Ridge Interbed; the Byron Interbed occurs between two flows of the Priest Rapids Member. The Mabton Interbed marks the contact between the Wanapum and Saddle Mountains Basalts. The thicknesses of the basalts and interbedded sediments were within expected limits. However, a small reverse fault was found in the Pomona Member flow top. This fault has three periods of

  13. Time-lapse downhole electrical resistivity monitoring of subsurface CO2 storage at the Maguelone shallow experimental site (Languedoc, France)

    Science.gov (United States)

    Denchik, Nataliya; Pezard, Philippe; Lofi, Johanna; Perroud, Hervé; Neyens, Denis; Luquot, Linda

    2015-04-01

    A shallow field experimental site for CO2 injection was established at Maguelone (Languedoc,France), in order to test in an integrated manner a suite of surface and downhole hydrogeophysical monitoring methods. The objective is to improve our understanding of gas transport in the shallow subsurface and to determine the sensitivity of CO2 monitoring systems for leakage detection. The site offers a natural laboratory to study the processes associated with CO2 injection in a clastic and clay-rich context saturated with saline fluids. Prior to CO2injection, three nitrogen (N2) injections were undertaken in 2012 to measure the site response to neutral gas injection. In 2013, a volume of 111 m3 of CO2 was injected during 3.5 hours at 15 meter depth. During each experiment, the gas plumes were successfully detected from pressure monitoring, time-lapse induction logging and downhole resistivity monitoring with downhole dipole-dipole arrays. Increases in resistivity are attributed to free gas propagation (either N2 or CO2) whereas decreases in resistivity correlate with CO2 dissolution in the pore fluid. Chemical analyses confirm this hypothesis with a decrease in pH and an increase in the concentration of dissolved species in the later case. The next stage of the project will be performing the CO2 injection experiments with improved monitoring schema using results of the present study. In perspective, besides of improving our understanding of gas transport in the shallow subsurface, the additional issues could not just show a capability of used geophysical and geochemical techniques to monitor the CO2 plume and to detect near-surface CO2 migration pathways, but to help quantifying potential CO2 migration.

  14. Cassba seismic test

    International Nuclear Information System (INIS)

    This paper presents the seismic test program applied to the mock-up CASSBA, a reduced scale mock-up of a 8 floor building, and presents the first results. After several seismic tests, realized at a level upper or equal to the calculated level, the mock-up, which presents a good resistance to strong seismic motions, has some closed and tilted cracks located in the 2 lower walls and some horizontal cracks located at the 5 lower floors. The extension of the frame is lower 0.3% and the mockup rises 12 mm during tests

  15. The seismicity of Sweden

    International Nuclear Information System (INIS)

    A new seismicity map of Sweden for the period 1951-1976 is presented. The map and the corresponding catalogue are based essentially on seismographic records made in the Nordic countries. The use of such records, in combination with improved methods of analysis, has made it possible not only to produce a more complete and more accurate seismicity map, but also to revise several earlier conclusions, based on macroseismic information only. In view of the great current interest in the seismicity of Sweden, from both the engineering and the tectonophysical viewpoints, a summary is given of the pertinent papers produced at the Seismological Institute, Uppsala. (author)

  16. Influence of axial deformation of pipe string on down-hole operation%管柱轴向变形对井下作业的影响

    Institute of Scientific and Technical Information of China (English)

    李旭阳

    2016-01-01

    This paper reviews the impact of axial deformation of string on different down-hole operating conditions along with the importance of the calculation on axial deformation for the establishment of calculation model of down-hole string axial deformation theory.%综合分析了管柱轴向变形对井下不同作业工况的影响,阐述了计算井下作业管柱轴向变形的重要性,为建立井下作业管柱轴向变形理论计算模型做了很好的铺垫。

  17. Research on seismic stress triggering

    Institute of Scientific and Technical Information of China (English)

    万永革; 吴忠良; 周公威; 黄静; 秦立新

    2002-01-01

    This paper briefly reviews basic theory of seismic stress triggering. Recent development on seismic stress triggering has been reviewed in the views of seismic static and dynamic stress triggering, application of viscoelastic model in seismic stress triggering, the relation between earthquake triggering and volcanic eruption or explosion, other explanation of earthquake triggering, etc. And some suggestions for further study on seismic stress triggering in near future are given.

  18. Two-Dimensional Fiber Positioning and Clamping Device for Product-Internal Microassembly

    NARCIS (Netherlands)

    Henneken, V.A.; Sassen, W.P.; Van der Vlist, W.; Wien, W.H.A.; Tichem, M.; Sarro, P.M.

    2008-01-01

    In this paper, we present a microelectromechanical systems-based two-degrees-of-freedom positioning device combined with a clamping structure for positioning and constraining an optical fiber. The fiber position can be controlled in the two directions perpendicular to the fiber axis using two specif

  19. ELECTROCARDIOGRAPHIC RESPONSES OF RAT FETUSES WITH CLAMPED OR INTACT UMBILICAL CORDS TO ACUTE MATERNAL UTERINE ISCHEMIA

    Science.gov (United States)

    Uterine ischemia results in severe cardiac disturbances in the fetus. It has been postulated that these effects are due to interaction with the ischemic uterus or placenta and not due to hypoxia or build up of metabolites in the fetus. The fetal cardiac responses to uterine clamp...

  20. The Response Clamp: Functional characterization of neural systems using closed-loop control

    Directory of Open Access Journals (Sweden)

    Avner Wallach

    2013-01-01

    Full Text Available The voltage clamp method, pioneered by Hodgkin, Huxley and Katz, laid the foundations to neurophysiological research. Its core rationale is the use of closed-loop control as a tool for system characterization. A recently introduced method, the response clamp, extends the voltage clamp rationale to the functional, phenomenological level. The method consists of on-line estimation of a response variable of interest (e.g. the probability of response or its latency and a simple feedback control mechanism designed to tightly converge this variable towards a desired trajectory. In the present contribution I offer a perspective on this novel method and its applications in the broader context of system identification and characterization. First, I demonstrate how internal state variables are exposed using the method, and how the use of several controllers may allow for a detailed, multi-variable characterization of the system. Second, I discuss three different categories of applications of the method: (i exploration of intrinsically generated dynamics, (ii exploration of extrinsically generated dynamics and (iii generation of input-output trajectories. The relation of these categories to similar uses in the voltage clamp and other techniques is also discussed. Finally, I discuss the method’s limitations, as well as its possible synthesis with existing complementary approaches.

  1. Early vs delayed clamping of the umbilical cord in full term, preterm and very preterm infants

    DEFF Research Database (Denmark)

    Moller, N.K.; Weber, T.

    2008-01-01

    Randomized studies from 2006 and two meta-analyses published in 2007 agree that clamping of the umbilical cord can be delayed. For the preterm and very preterm infant benefits include less need for blood transfusion and less morbidity, especially for the very preterm male infant. For the term...

  2. Experimental and numerical analysis of clamped joints in front motorbike suspensions

    Directory of Open Access Journals (Sweden)

    Vincenzi N.

    2010-06-01

    Full Text Available Clamped joints are shaft-hub connections used, as an instance, in front motorbike suspensions to lock the steering plates with the legs and the legs with the wheel pin, by means of one or two bolts. The preloading force, produced during the tightening process, should be evaluated accurately, since it must lock safely the shaft, without overcoming the yielding point of the hub. Firstly, friction coefficients have been evaluated on “ad-hoc designed” specimens, by applying the Design of Experiment approach: the applied tightening torque has been precisely related to the imposed preloading force. Then, the tensile state of clamps have been evaluated both via FEM and by leveraging some design formulae proposed by the Authors as function of the preloading force and of the clamp geometry. Finally, the results have been compared to those given by some strain gauges applied on the tested clamps: the discrepancies between numerical analyses, the design formulae and the experimental results remains under a threshold of 10%.

  3. Two simple finite element methods for Reissner--Mindlin plates with clamped boundary condition

    OpenAIRE

    Bishnu P. Lamichhane

    2013-01-01

    We present two simple finite element methods for the discretization of Reissner--Mindlin plate equations with {\\em clamped} boundary condition. These finite element methods are based on discrete Lagrange multiplier spaces from mortar finite element techniques. We prove optimal a priori error estimates for both methods.

  4. Trans-Z-source and Γ-Z-source neutral-point-clamped inverters

    DEFF Research Database (Denmark)

    Wei, Mo; Loh, Poh Chiang; Blaabjerg, Frede;

    2015-01-01

    Z-source neutral-point-clamped (NPC) inverters are earlier proposed for obtaining voltage buck-boost and three-level switching simultaneously. Their performances are, however, constrained by a trade-off between their input-to-output gain and modulation ratio. This trade-off can lead to high...

  5. An Efficient Single Impedance Network Three Level Z-Source Neutral Point Clamped Inverter

    Directory of Open Access Journals (Sweden)

    C.L.Kuppuswamy

    2012-07-01

    Full Text Available A multilevel inverter can eliminate the need for the step-up transformer and reduce the harmonics produced by the inverter. Although the multilevel inverter structure was initially introduced as a means of reducing the output waveform harmonic content, it was found that the dc bus voltage could be increased beyond the voltage rating of an individual power device by the use of a voltage clamping network consisting of diodes. By using voltage clamping techniques, the system KV rating can be extended beyond limits of an individual device. This paper presents Impedance (Z Source Neutral Point Clamped (NPC three level inverter with reduced number of impedance source networks and clamping diodes for non-linear loads. Earlier for a three level NPC inverter two separate impedance networks were used. But to reduce the circuit complexity without compromising with output requirement; only one impedance network is used and to substantiate this, the simulation of proposed circuit is carried out using MATLAB/Simulink for a 48 V input supply. A prototype is developed for an input voltage of 26V and the experimental results for the same are presented in this paper.

  6. A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode

    NARCIS (Netherlands)

    Jergic, Slobodan; Horan, Nicholas P.; Elshenawy, Mohamed M.; Mason, Claire E.; Urathamakul, Thitima; Ozawa, Kiyoshi; Robinson, Andrew; Goudsmits, Joris M. H.; Wang, Yao; Pan, Xuefeng; Beck, Jennifer L.; van Oijen, Antoine M.; Huber, Thomas; Hamdan, Samir M.; Dixon, Nicholas E.

    2013-01-01

    Processive DNA synthesis by the alpha epsilon theta core of the Escherichia coli Pol III replicase requires it to be bound to the beta(2) clamp via a site in the a polymerase subunit. How the epsilon proofreading exonuclease subunit influences DNA synthesis by alpha was not previously understood. In

  7. Common voltage eliminating of SVM diode clamping three-level inverter connected to grid

    DEFF Research Database (Denmark)

    Guo, Yougui; Zeng, Ping; Zhu, Jieqiong;

    2011-01-01

    A novel method of common voltage eliminating is put forward for SVM diode clamping three-level inverter connected to grid by calculation of common voltage of its various switching states. PLECS is used to model this three-level inverter connected to grid and good results are obtained. First...

  8. Seismic data acquisition systems

    International Nuclear Information System (INIS)

    Details of seismic data acquisition systems developed at the Bhabha Atomic Research Centre, Bombay are reported. The seismic signals acquired belong to different signal bandwidths in the band from 0.02 Hz to 250 Hz. All these acquisition systems are built around a unique technique of recording multichannel data on to a single track of an audio tape and in digital form. Techniques of how these signals in different bands of frequencies were acquired and recorded are described. Method of detecting seismic signals and its performance is also discussed. Seismic signals acquired in different set-ups are illustrated. Time indexing systems for different set-ups and multichannel waveform display systems which form essential part of the data acquisition systems are also discussed. (author). 13 refs., 6 figs., 1 tab

  9. Seismic Fault Preserving Diffusion

    CERN Document Server

    Lavialle, Olivier; Germain, Christian; Donias, Marc; Guillon, Sebastien; Keskes, Naamen; Berthoumieu, Yannick

    2007-01-01

    This paper focuses on the denoising and enhancing of 3-D reflection seismic data. We propose a pre-processing step based on a non linear diffusion filtering leading to a better detection of seismic faults. The non linear diffusion approaches are based on the definition of a partial differential equation that allows us to simplify the images without blurring relevant details or discontinuities. Computing the structure tensor which provides information on the local orientation of the geological layers, we propose to drive the diffusion along these layers using a new approach called SFPD (Seismic Fault Preserving Diffusion). In SFPD, the eigenvalues of the tensor are fixed according to a confidence measure that takes into account the regularity of the local seismic structure. Results on both synthesized and real 3-D blocks show the efficiency of the proposed approach.

  10. BUILDING 341 Seismic Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Halle, J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-06-15

    The Seismic Evaluation of Building 341 located at Lawrence Livermore National Laboratory in Livermore, California has been completed. The subject building consists of a main building, Increment 1, and two smaller additions; Increments 2 and 3.

  11. Reproducibility in Seismic Imaging

    Directory of Open Access Journals (Sweden)

    González-Verdejo O.

    2012-04-01

    Full Text Available Within the field of exploration seismology, there is interest at national level of integrating reproducibility in applied, educational and research activities related to seismic processing and imaging. This reproducibility implies the description and organization of the elements involved in numerical experiments. Thus, a researcher, teacher or student can study, verify, repeat, and modify them independently. In this work, we document and adapt reproducibility in seismic processing and imaging to spread this concept and its benefits, and to encourage the use of open source software in this area within our academic and professional environment. We present an enhanced seismic imaging example, of interest in both academic and professional environments, using Mexican seismic data. As a result of this research, we prove that it is possible to assimilate, adapt and transfer technology at low cost, using open source software and following a reproducible research scheme.

  12. Seismic Fault Preserving Diffusion

    OpenAIRE

    Lavialle, Olivier; Pop, Sorin; Germain, Christian; Donias, Marc; Guillon, Sebastien; Keskes, Naamen; Berthoumieu, Yannick

    2007-01-01

    This paper focuses on the denoising and enhancing of 3-D reflection seismic data. We propose a pre-processing step based on a non linear diffusion filtering leading to a better detection of seismic faults. The non linear diffusion approaches are based on the definition of a partial differential equation that allows us to simplify the images without blurring relevant details or discontinuities. Computing the structure tensor which provides information on the local orientation of the geological...

  13. Lithological controls on gas hydrate saturation: Insights from signal classification of NMR downhole data

    Science.gov (United States)

    Bauer, Klaus; Kulenkampff, Johannes; Henninges, Jan; Spangenberg, Erik

    2016-04-01

    Nuclear magnetic resonance (NMR) downhole data are analyzed with a new strategy to study gas hydrate-bearing sediments in the Mackenzie Delta (NW Canada). NMR logging is a powerful tool to study geological reservoir formations. The measurements are based on interactions between the magnetic moments of protons in geological formation water and an external magnetic field. Inversion of the measured raw data provides so-called transverse relaxation time (T2) distribution curves or spectra. Different parts of the T2 curve are related with distinct pore radii and corresponding fluid components. A common practice in the analysis of T2 distribution curves is to extract single-valued parameters such as apparent total porosity. Moreover, the derived total NMR apparent porosity and the gamma-gamma density log apparent porosity can be combined to estimate gas hydrate saturation in hydrate-bearing sediments. To avoid potential loss of information, in our new approach we analyze the entire T2 distribution curves as quasi-continuous signals to characterize the rock formation. The approach is applied to NMR data measured in gas hydrate research well Mallik 5L-38. We use self-organizing maps, a neural network clustering technique, to subdivide the data set of NMR T2 distribution curves into classes with a similar and distinctive signal shape. The method includes (1) preparation of data vectors, (2) unsupervised learning, (3) cluster definition, and (4) classification and depth mapping of all NMR signals. Each signal class thus represents a specific pore size distribution which can be interpreted in terms of distinct lithologies and reservoir types. A key step in the interpretation strategy is to reconcile the NMR classes with other log data not considered in the clustering analysis, such as gamma ray, photo-electric factor, hydrate saturation, and other logs. Our results defined six main lithologies within the target zone. Gas hydrate layers were recognized by their low signal

  14. Seismic Consequence Abstraction

    Energy Technology Data Exchange (ETDEWEB)

    M. Gross

    2004-10-25

    The primary purpose of this model report is to develop abstractions for the response of engineered barrier system (EBS) components to seismic hazards at a geologic repository at Yucca Mountain, Nevada, and to define the methodology for using these abstractions in a seismic scenario class for the Total System Performance Assessment - License Application (TSPA-LA). A secondary purpose of this model report is to provide information for criticality studies related to seismic hazards. The seismic hazards addressed herein are vibratory ground motion, fault displacement, and rockfall due to ground motion. The EBS components are the drip shield, the waste package, and the fuel cladding. The requirements for development of the abstractions and the associated algorithms for the seismic scenario class are defined in ''Technical Work Plan For: Regulatory Integration Modeling of Drift Degradation, Waste Package and Drip Shield Vibratory Motion and Seismic Consequences'' (BSC 2004 [DIRS 171520]). The development of these abstractions will provide a more complete representation of flow into and transport from the EBS under disruptive events. The results from this development will also address portions of integrated subissue ENG2, Mechanical Disruption of Engineered Barriers, including the acceptance criteria for this subissue defined in Section 2.2.1.3.2.3 of the ''Yucca Mountain Review Plan, Final Report'' (NRC 2003 [DIRS 163274]).

  15. Seismic Consequence Abstraction

    International Nuclear Information System (INIS)

    The primary purpose of this model report is to develop abstractions for the response of engineered barrier system (EBS) components to seismic hazards at a geologic repository at Yucca Mountain, Nevada, and to define the methodology for using these abstractions in a seismic scenario class for the Total System Performance Assessment - License Application (TSPA-LA). A secondary purpose of this model report is to provide information for criticality studies related to seismic hazards. The seismic hazards addressed herein are vibratory ground motion, fault displacement, and rockfall due to ground motion. The EBS components are the drip shield, the waste package, and the fuel cladding. The requirements for development of the abstractions and the associated algorithms for the seismic scenario class are defined in ''Technical Work Plan For: Regulatory Integration Modeling of Drift Degradation, Waste Package and Drip Shield Vibratory Motion and Seismic Consequences'' (BSC 2004 [DIRS 171520]). The development of these abstractions will provide a more complete representation of flow into and transport from the EBS under disruptive events. The results from this development will also address portions of integrated subissue ENG2, Mechanical Disruption of Engineered Barriers, including the acceptance criteria for this subissue defined in Section 2.2.1.3.2.3 of the ''Yucca Mountain Review Plan, Final Report'' (NRC 2003 [DIRS 163274])

  16. Earth modeling and estimation of the local seismic ground motion due to site geology in complex volcanoclastic areas

    Directory of Open Access Journals (Sweden)

    V. Di Fiore

    2002-06-01

    Full Text Available Volcanic areas often show complex behaviour as far as seismic waves propagation and seismic motion at surface are concerned. In fact, the finite lateral extent of surface layers such as lava flows, blocks, differential welding and/or zeolitization within pyroclastic deposits, introduces in the propagation of seismic waves effects such as the generation of surface waves at the edge, resonance in lateral direction, diffractions and scattering of energy, which tend to modify the amplitude as well as the duration of the ground motion. The irregular topographic surface, typical of volcanic areas, also strongly influences the seismic site response. Despite this heterogeneity, it is unfortunately a common geophysical and engineering practice to evaluate even in volcanic environments the subsurface velocity field with monodimensional investigation method (i.e. geognostic soundings, refraction survey, down-hole, etc. prior to the seismic site response computation which in a such cases is obviously also made with 1D algorithms. This approach often leads to highly inaccurate results. In this paper we use a different approach, i.e. a fully 2D P-wave Çturning rayÈ tomographic survey followed by 2D seismic site response modeling. We report here the results of this approach in three sites located at short distance from Mt. Vesuvius and Campi Flegrei and characterized by overburdens constituted by volcanoclastic deposits with large lateral and vertical variations of their elastic properties. Comparison between 1D and 2D Dynamic Amplification Factor shows in all reported cases entirely different results, both in terms of peak period and spectral contents, as expected from the clear bidimensionality of the geological section. Therefore, these studies suggest evaluating carefully the subsoil geological structures in areas characterized by possible large lateral and vertical variations of the elastic properties in order to reach correct seismic site response

  17. Seismic response of flexible clyindrical tanks

    International Nuclear Information System (INIS)

    An experimental study of the seismic behaviour of thin shell circular cylindrical liquid storage tanks is described. The investigation was planned to evaluate the adequacy of present methods of tank design, and was conducted using the Earthquake Simulator Facility of the University of California, Berkeley. The model tank considered in this paper was 6 ft high by 12 ft in diameter, and was welded from thin sheet aluminium to simulate a steel tank 36 feet in diameter. Results of the experiments demonstrate that fluid pressures included both impulsive and convective components, and that the wave sloshing followed basic theory quite closely.But it also was apparent that the tank flexibility influenced the hydrodynamic pressures, as indicated by pressure amplification in the clamped tank, and by a total change of pressure history in the unclamped case. Significant out of round distortions of the tank were developed, of a three lobe form for the free base case and with four lobes in the fixed base case. Uplift of the tank base was closely related to the out-of-round deformation of the unanchored tank, whereas initial eccentricities apparently caused the section distortions in the anchored system. Stresses in the tank wall do not follow the expected pattern of response to overturning moment; instead they seem to be mainly associated with the section distortions. At present there is no analytical procedure for predicting these distortions. (Auth.)

  18. Regulatory perspective of seismic issues

    International Nuclear Information System (INIS)

    Some of the current seismic issues facing the nuclear power industry, such as seismic design criteria (USI A-40), seismic qualification of equipment in operating nuclear power plants (USI A-46), eastern United States seismicity, operating basis earthquake (OBE) exceedance criteria, seismic instrumentation, post OBE inspection of nuclear power plants, anchor bolts too close to a free edge, seismic margins of plants, and the potential for external events to cause severe accidents, are presented and the NRC's perspective on the resolution of these issues are discussed. (orig.)

  19. Heavy oil recovery process: Conceptual engineering of a downhole methanator and preliminary estimate of facilities cost for application to North Slope Alaska

    Energy Technology Data Exchange (ETDEWEB)

    Gondouin, M.

    1991-10-31

    The West Sak (Upper Cretaceous) sands, overlaying the Kuparuk field, would rank among the largest known oil fields in the US, but technical difficulties have so far prevented its commercial exploitation. Steam injection is the most successful and the most commonly-used method of heavy oil recovery, but its application to the West Sak presents major problems. Such difficulties may be overcome by using a novel approach, in which steam is generated downhole in a catalytic Methanator, from Syngas made at the surface from endothermic reactions (Table 1). The Methanator effluent, containing steam and soluble gases resulting from exothermic reactions (Table 1), is cyclically injected into the reservoir by means of a horizontal drainhole while hot produced fluids flow form a second drainhole into a central production tubing. The downhole reactor feed and BFW flow downward to two concentric tubings. The large-diameter casing required to house the downhole reactor assembly is filled above it with Arctic Pack mud, or crude oil, to further reduce heat leaks. A quantitative analysis of this production scheme for the West Sak required a preliminary engineering of the downhole and surface facilities and a tentative forecast of well production rates. The results, based on published information on the West Sak, have been used to estimate the cost of these facilities, per daily barrel of oil produced. A preliminary economic analysis and conclusions are presented together with an outline of future work. Economic and regulatory conditions which would make this approach viable are discussed. 28 figs.

  20. Seismicity and seismic monitoring in the Asse salt mine

    International Nuclear Information System (INIS)

    Seismicity analyses are made in order to assess the safety of candidate sites for ultimate disposal of hazardous wastes. The report in hand reviews the seismicity history of the Asse salt mine and presents recent results of a measuring campaign made in the area. The monitoring network installed at the site supplies data and information on the regional seismicity, on seismic amplitudes under ground and above ground, and on microseismic activities. (DG)

  1. Seismic forecast using geostatistics

    International Nuclear Information System (INIS)

    The main idea of this research direction consists in the special way of constructing a new type of mathematical function as being a correlation between a computed statistical quantity and another physical quantity. This type of function called 'position function' was taken over by the authors of this study in the field of seismology with the hope of solving - at least partially - the difficult problem of seismic forecast. The geostatistic method of analysis focuses on the process of energy accumulation in a given seismic area, completing this analysis by a so-called loading function. This function - in fact a temporal function - describes the process of energy accumulation during a seismic cycle from a given seismic area. It was possible to discover a law of evolution of the seismic cycles that was materialized in a so-called characteristic function. This special function will help us to forecast the magnitude and the occurrence moment of the largest earthquake in the analysed area. Since 2000, the authors have been evolving to a new stage of testing: real - time analysis, in order to verify the quality of the method. There were five large earthquakes forecasts. (authors)

  2. Pickering seismic safety margin

    International Nuclear Information System (INIS)

    A study was conducted to recommend a methodology for the seismic safety margin review of existing Canadian CANDU nuclear generating stations such as Pickering A. The purpose of the seismic safety margin review is to determine whether the nuclear plant has sufficient seismic safety margin over its design basis to assure plant safety. In this review process, it is possible to identify the weak links which might limit the seismic performance of critical structures, systems and components. The proposed methodology is a modification the EPRI (Electric Power Research Institute) approach. The methodology includes: the characterization of the site margin earthquake, the definition of the performance criteria for the elements of a success path, and the determination of the seismic withstand capacity. It is proposed that the margin earthquake be established on the basis of using historical records and the regional seismo-tectonic and site specific evaluations. The ability of the components and systems to withstand the margin earthquake is determined by database comparisons, inspection, analysis or testing. An implementation plan for the application of the methodology to the Pickering A NGS is prepared

  3. Free Vibration Analysis of an Alround-Clamped Rectangular Thin Orthotropic Plate Using Taylor-Mclaurin Shape Function

    Directory of Open Access Journals (Sweden)

    D.O Onwuka

    2016-06-01

    Full Text Available A comprehensive free vibration analysis of an alround-clamped rectangular thin or thotropic plate, was carried out using Taylor- Mclaurin shape function, and Ritz method. The Taylor-Mclaurin shape function truncated at the fourth term satisfied all the boundary conditions of the alround-clamped thin orthotropic plate. The shape function was substituted into the total energy functional, which was subsequently minimized. From the minimized equation, the natural frequency equation for the clamped plate, was derived. The resulting equation was used to calculate fundamental natural frequencies of the clamped plate for various aspect ratios, p and different combinations of flexural rigidity ratios, φ. The fundamental frequencies for a clamped plate vibrating in the first mode are given in Tables1-5, for different flexural rigidities, φ and aspect ratios varying from 0.1 to 2 at increments of 0.1. The average percentage difference in the values of natural frequency for the flexural rigidity ratios, φ1, φ2, and φ3, are 1.532%,1.367% and 1.425% for different values of the aspect ratio, = ; and 1.149%, 1.506% and……..for different values of the aspect ratio, = . These average percentage differences indicate that the formulated deflection function for the clamped plate, is a very good approximation to the exact deflection function of the free vibration of a clamped rectangular thin orthotropic plate

  4. VSP in crystalline rocks - from downhole velocity profiling to 3-D fracture mapping

    International Nuclear Information System (INIS)

    VSP surveys have been carried out at several potential nuclear waste disposal sites in Finland since the mid 80s. To date, more than 200 three-component profiles have been measured. The main purpose of the surveys was to detect fracture zones in the crystalline bedrock and to determine their position. Most seismic events could be linked to zones of increased fracturing observed in the borehole logs. The more pronounced seismic reflectors could be correlated with hydrogeologically significant zones, which have been the main targets in the investigations. Processing and interpretation methods have been developed specifically for VSP surveys in crystalline rocks: Weak reflections from thin fracture zones are enhanced by multi-channel filtering techniques based on the Radon transform. The position and orientation of the fracture zones are determined by polarisation analysis and by combining data from several shot points. The compilation of the results from several boreholes gives a comprehensive image of the fracture zones at the scale of the whole site. The discussion of the methodology is based on examples from the Olkiluoto site, in SW Finland

  5. Application of Genetic Algorithms in Seismic Tomography

    Science.gov (United States)

    Soupios, Pantelis; Akca, Irfan; Mpogiatzis, Petros; Basokur, Ahmet; Papazachos, Constantinos

    2010-05-01

    application of hybrid genetic algorithms in seismic tomography is examined and the efficiency of least squares and genetic methods as representative of the local and global optimization, respectively, is presented and evaluated. The robustness of both optimization methods has been tested and compared for the same source-receiver geometry and characteristics of the model structure (anomalies, etc.). A set of seismic refraction synthetic (noise free) data was used for modeling. Specifically, cross-well, down-hole and typical refraction studies using 24 geophones and 5 shoots were used to confirm the applicability of the genetic algorithms in seismic tomography. To solve the forward modeling and estimate the traveltimes, the revisited ray bending method was used supplemented by an approximate computation of the first Fresnel volume. The root mean square (rms) error as the misfit function was used and calculated for the entire random velocity model for each generation. After the end of each generation and based on the misfit of the individuals (velocity models), the selection, crossover and mutation (typical process steps of genetic algorithms) were selected continuing the evolution theory and coding the new generation. To optimize the computation time, since the whole procedure is quite time consuming, the Matlab Distributed Computing Environment (MDCE) was used in a multicore engine. During the tests, we noticed that the fast convergence that the algorithm initially exhibits (first 5 generations) is followed by progressively slower improvements of the reconstructed velocity models. Thus, to improve the final tomographic models, a hybrid genetic algorithm (GA) approach was adopted by combining the GAs with a local optimization method after several generations, on the basis of the convergence of the resulting models. This approach is shown to be efficient, as it directs the solution search towards a model region close to the global minimum solution.

  6. Attenuation law of seismic waves in technical seismicity

    OpenAIRE

    B. Pandula; J. Kondela; K. Pachocka

    2012-01-01

    Blasting operations have positive and negative effects as well. Vibration intensity and seismic safety associated to the blasting operations are highly actual and challenging problem. The presented article describes the results of the analysis and the methodology of evaluation of objects` seismic safety during blasting operations by application of attenuation law of the seismic waves.

  7. Comparison between symmetrical and asymmetrical single phase multilevel inverter with diode-clamped topology

    DEFF Research Database (Denmark)

    Blaabjerg, Frede; Nami, A.; Zare, F.; Ledwich, G.; Ghosh, A.

    In this paper, a different configuration based on different DC bus voltage for a diode-clamped multilevel inverter has been presented. Two different symmetrical and asymmetrical arrangements of a four-level diode clamped inverters have been compared, in order to find an optimum arrangement with...... lower switching losses and optimised output voltage quality. The optimised asymmetrical arrangement has been compared with a conventional four- level inverter. The comparison results show that an asymmetrical configuration can obtain more voltage levels in output voltage with same number of component...... compared with the conventional four-level inverter and this will lead to the reduction of harmonic content of output voltage. A predictive current control technique has been carried out to verify the viability of new configuration. The advantages of this control method are simplicity and applicability for...

  8. High Efficiency Interleaved Active Clamped Dc-Dc Converter with Fuel Cell for High Voltage Applications

    Directory of Open Access Journals (Sweden)

    Sona P

    2014-02-01

    Full Text Available A high efficiency interleaved ZVS active clamped current fed dc-dc converter is proposed in this paper specially used for fuel cell applications. As the fuel cell output is very low we are in need of a step up dc-dc converter. Here a current fed dc-dc converter is used. Two current fed dc-dc converters are interleaved by connecting their inputs in parallel and outputs in series. With this proposed methodology input current ripples in the fuel cell stacks can be reduced and a regulated output voltage ripples can be obtained. The active clamping circuit used in this model absorbs the turn off voltage spikes hence low voltage devices with low on state resistance can be used.Voltage doubler circuits will give double the output voltage than normal with smaller transformer turns ratio and flexibility. The proposed method is simulated in MATLAB for verifying the accuracy of the proposed design.

  9. Short Hypoxia Does not Affect Plasma Leptin in Healthy Men under Euglycemic Clamp Conditions

    Directory of Open Access Journals (Sweden)

    Andre Schmoller

    2009-01-01

    Full Text Available Leptin is involved in the endocrine control of energy expenditure and body weight regulation. Previous studies emphasize a relationship between hypoxic states and leptin concentrations. The aim of this study was to investigate the effects of acute hypoxia on leptin concentrations in healthy subjects. We examined 14 healthy men. Hypoxic conditions were induced by decreasing oxygen saturation to 75% for 30 minutes. Plasma leptin concentrations were determined at baseline, after 3 hours of euglycemic clamping, during hypoxia, and repeatedly the following 2.5 hours thereafter. Our results show an increase of plasma leptin concentrations in the course of 6 hours of hyperinsulinemic-euglycemic clamping which may reflect diurnal rhythmicity. Notwithstanding, there was no difference between levels of leptin in the hypoxic and the normoxic condition (=.2. Since we did not find any significant changes in leptin responses upon hypoxia, plasma leptin levels do not seem to be affected by short hypoxic episodes of moderate degree.

  10. Membrane tether formation from voltage-clamped outer hair cells using optical tweezers

    Science.gov (United States)

    Qian, Feng; Ermilov, Sergey A.; Murdock, David R.; Brownell, William E.; Anvari, Bahman

    2004-06-01

    Outer hair cells contribute an active mechanical feedback to the vibrations of the cochlear structures resulting in the high sensitivity and frequency selectivity of normal hearing. We have designed and implemented a novel experimental setup that combines optical tweezers with patch-clamp apparatus to investigate the electromechanical properties of cellular plasma membranes. A micron-size bead trapped by the optical tweezers is brought in contact with the membrane of a voltage-clamped cell, and subsequently moved away to form a plasma membrane tether. Bead displacement during tether elongation is monitored by a quadrant photodetector to obtain time-resolved measurements of the tethering force. Salient information associated with the mechanical properties of the membrane tether can thus be obtained. Tethers can be pulled from the cell membrane at different holding potentials, and the tether force response can be measured while changing transmembrane potential. Experimental results from outer hair cells and human embryonic kidney cells are presented.

  11. Detailed Theoretical Model for Adjustable Gain-Clamped Semiconductor Optical Amplifier

    Directory of Open Access Journals (Sweden)

    Lin Liu

    2012-01-01

    Full Text Available The adjustable gain-clamped semiconductor optical amplifier (AGC-SOA uses two SOAs in a ring-cavity topology: one to amplify the signal and the other to control the gain. The device was designed to maximize the output saturated power while adjusting gain to regulate power differences between packets without loss of linearity. This type of subsystem can be used for power equalisation and linear amplification in packet-based dynamic systems such as passive optical networks (PONs. A detailed theoretical model is presented in this paper to simulate the operation of the AGC-SOA, which gives a better understanding of the underlying gain clamping mechanics. Simulations and comparisons with steady-state and dynamic gain modulation experimental performance are given which validate the model.

  12. Simplified Estimating Method for Shock Response Spectrum Envelope of V-Band Clamp Separation Shock

    Science.gov (United States)

    Iwasa, Takashi; Shi, Qinzhong

    A simplified estimating method for the Shock Response Spectrum (SRS) envelope at the spacecraft interface near the V-band clamp separation device has been established. This simplified method is based on the pyroshock analysis method with a single degree of freedom (D.O.F) model proposed in our previous paper. The parameters required in the estimating method are only geometrical information of the interface and a tension of the V-band clamp. According to the use of these parameters, a simplified calculation of the SRS magnitude at the knee frequency is newly proposed. By comparing the estimation results with actual pyroshock test results, it was verified that the SRS envelope estimated with the simplified method appropriately covered the pyroshock test data of the actual space satellite systems except some specific high frequency responses.

  13. Study of transmembrane La3+ movement in rat ventricular myocytes by the patch-clamp technique

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    We have studied transmembrane La3+ movement in rat ventricular myocytes for the first time by using the whole-cell patch-clamp recording mode. La3+ (0.01-5.0 mmol/L) could not bring out inward currents through the L-type calcium channel in rat ventricular myocytes, while it could enter the cells by the same way carried by 1μmol/L ionomycin. When the outward Na+ concentration gradient is formed, La3+ can enter the cells via Na-Ca exchange, and the exchange currentsincrease with the increase of external La3+ concentrations. But compared with Na-Ca exchange currents in the same concentration, the former is only 14%-38% of the latter. The patch-clamp experiment indicates that La3+ normally can not enter ventricular myocytes through L-type calcium channel, but it can enter the cells via Na-Ca exchange.

  14. Experimental validation of new mathematical solutions for orthotropic plates with clamped edges

    Science.gov (United States)

    Sprinťu, Iuliana; Roateşi, Simona

    2013-10-01

    This paper deals with analytical solutions for the bending deformation of rectangular orthotropic elastic composite plates with various boundary conditions. The models are based on the classical laminated plate theory (CLPT). The Ritz method, in conjunction with the weighted residue method for the coefficients calculation is used to analytically determine the bending solutions of orthotropic laminated plates subjected to uniform pressure on the bottom laminate, having clamped edges or possessing two opposite edges simply supported and the remaining two edges clamped, respectively. Numerical examples of laminated plates considering similar boundary value problems as treated analytically are presented. It is presented the experimental device and the experimental test results, as well. Thorough comparison between analytical solutions, numerical results and experimental data is performed and a good agreement is obtained.

  15. Anthropomorphizing the Mouse Cardiac Action Potential via a Novel Dynamic Clamp Method

    Science.gov (United States)

    Ahrens-Nicklas, Rebecca C.; Christini, David J.

    2009-01-01

    Abstract Interspecies differences can limit the translational value of excitable cells isolated from model organisms. It can be difficult to extrapolate from a drug- or mutation-induced phenotype in mice to human pathophysiology because mouse and human cardiac electrodynamics differ greatly. We present a hybrid computational-experimental technique, the cell-type transforming clamp, which is designed to overcome such differences by using a calculated compensatory current to convert the macroscopic electrical behavior of an isolated cell into that of a different cell type. We demonstrate the technique's utility by evaluating drug arrhythmogenicity in murine cardiomyocytes that are transformed to behave like human myocytes. Whereas we use the cell-type transforming clamp in this work to convert between mouse and human electrodynamics, the technique could be adapted to convert between the action potential morphologies of any two cell types of interest. PMID:19917221

  16. Duration of Cord Clamping and Neonatal Outcomes in Very Preterm Infants.

    Directory of Open Access Journals (Sweden)

    Dongli Song

    Full Text Available Delayed cord clamping (DCC, ≥30 s increases blood volume in newborns and is associated with fewer blood transfusions and short-term neonatal complications. The optimal timing of cord clamping for very preterm infants should maximize placental transfusion without interfering with stabilization and resuscitation.We compared the effect of different durations of DCC, 30-45 s vs. 60-75 s, on delivery room (DR and neonatal outcomes in preterm infants 48 hours of antenatal steroid exposure. There was no difference between the two groups in neonatal death, intraventricular hemorrhage, chronic lung disease, late onset sepsis, necrotizing enterocolitis and severe retinopathy of prematurity.In this study cohort increasing DCC duration from 30-45 s to 60-75 s is associated with decreased hypothermia on admission, neonatal respiratory interventions and red blood cell transfusions without increase in neonatal mortality and morbidities.

  17. Magnitude correlations in global seismicity

    International Nuclear Information System (INIS)

    By employing natural time analysis, we analyze the worldwide seismicity and study the existence of correlations between earthquake magnitudes. We find that global seismicity exhibits nontrivial magnitude correlations for earthquake magnitudes greater than Mw6.5.

  18. Controllable seismic source

    Energy Technology Data Exchange (ETDEWEB)

    Gomez, Antonio; DeRego, Paul Jeffrey; Ferrell, Patrick Andrew; Thom, Robert Anthony; Trujillo, Joshua J.; Herridge, Brian

    2015-09-29

    An apparatus for generating seismic waves includes a housing, a strike surface within the housing, and a hammer movably disposed within the housing. An actuator induces a striking motion in the hammer such that the hammer impacts the strike surface as part of the striking motion. The actuator is selectively adjustable to change characteristics of the striking motion and characteristics of seismic waves generated by the impact. The hammer may be modified to change the physical characteristics of the hammer, thereby changing characteristics of seismic waves generated by the hammer. The hammer may be disposed within a removable shock cavity, and the apparatus may include two hammers and two shock cavities positioned symmetrically about a center of the apparatus.

  19. Controllable seismic source

    Energy Technology Data Exchange (ETDEWEB)

    Gomez, Antonio; DeRego, Paul Jeffrey; Ferrel, Patrick Andrew; Thom, Robert Anthony; Trujillo, Joshua J.; Herridge, Brian

    2014-08-19

    An apparatus for generating seismic waves includes a housing, a strike surface within the housing, and a hammer movably disposed within the housing. An actuator induces a striking motion in the hammer such that the hammer impacts the strike surface as part of the striking motion. The actuator is selectively adjustable to change characteristics of the striking motion and characteristics of seismic waves generated by the impact. The hammer may be modified to change the physical characteristics of the hammer, thereby changing characteristics of seismic waves generated by the hammer. The hammer may be disposed within a removable shock cavity, and the apparatus may include two hammers and two shock cavities positioned symmetrically about a center of the apparatus.

  20. Microearthquakes and seismic risk

    International Nuclear Information System (INIS)

    Paper is presented in the nature of a review of the potential of microearthquake surveys in seismotectonic stc studies, particularly seismic-risk analyses. Miscroearthquakes are generally considered as shocks with magnitude less than 3. They radiate, in general, higher frequencies than larger earthquakes and therefore microearthquake seismographs must be sensitive to frequencies typically up to 30 or 40Hz. Mircroearthquakes are useful in seismotectonic studies because: (1) a greater number of small than large shocks occur in the same time, (2) extension to lower magnitudes of frequency-of-occurrence curves can yield more reliable seismic-risk statistics, (3) due to the reduced scale, both in space and time, very precise focal location is possible, often enabling mapping of fracture surfaces, (4) focal-mechanism solutions can further determine the fault surface and, in addition, the direction of slip, and (5) continuous monitoring of seismic activity is possible through the means of a permanent network of microearthquake systems. (author)

  1. Induced seismicity. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Segall, P.

    1997-09-18

    The objective of this project has been to develop a fundamental understanding of seismicity associated with energy production. Earthquakes are known to be associated with oil, gas, and geothermal energy production. The intent is to develop physical models that predict when seismicity is likely to occur, and to determine to what extent these earthquakes can be used to infer conditions within energy reservoirs. Early work focused on earthquakes induced by oil and gas extraction. Just completed research has addressed earthquakes within geothermal fields, such as The Geysers in northern California, as well as the interactions of dilatancy, friction, and shear heating, on the generation of earthquakes. The former has involved modeling thermo- and poro-elastic effects of geothermal production and water injection. Global Positioning System (GPS) receivers are used to measure deformation associated with geothermal activity, and these measurements along with seismic data are used to test and constrain thermo-mechanical models.

  2. The effect of adrenergic β2 receptor agonist on paraplegia following clamping of abdominal aorta

    OpenAIRE

    Lee, Bok Y.; Al-Waili, Noori; Butler, Glenn

    2011-01-01

    Introduction Surgical repair of an aortic aneurysm might be complicated by spinal cord injury and paraplegia. Since β-adrenoreceptor agonists showed neuroprotective effects, the study was designed to investigate the effect of clenbuterol on post-aortic clamping paraplegia and to identify if there is hyperemia associated with paraplegia. Material and methods Material and methods: Thirty rabbits were divided into two groups: 15 control and 15 experimental (given clenbuterol 9 mg in drinking wat...

  3. Ionic diffusion in voltage-clamped isolated cardiac myocytes. Implications for Na,K-pump studies.

    OpenAIRE

    Mogul, D J; Singer, D H; ten Eick, R E

    1989-01-01

    The whole-cell voltage-clamp technique employing electrolyte-filled micro-pipette suction electrodes is widely used to investigate questions requiring an electrophysiological approach. With this technique, the ionic composition of the cytosol is assumed to be strongly influenced (as result of diffusion) by the ionic composition of the solution contained in the electrode. If this assumption is valid for isolated cardiac myocytes, the technique would be particularly powerful for studying the de...

  4. Evidence for cooperativity between nicotinic acetylcholine receptors in patch clamp records.

    OpenAIRE

    Keleshian, A M; Edeson, R. O.; G.J. Liu; Madsen, B W

    2000-01-01

    It is often assumed that ion channels in cell membrane patches gate independently. However, in the present study nicotinic receptor patch clamp data obtained in cell-attached mode from embryonic chick myotubes suggest that the distribution of steady-state probabilities for conductance multiples arising from concurrent channel openings may not be binomial. In patches where up to four active channels were observed, the probabilities of two or more concurrent openings were greater than expected,...

  5. Direct Tensile Strength of Steel Fiber Reinforced Concrete by Using Clamping Grips

    OpenAIRE

    ISHIGURO, Satoru; NAKAYA, Mitsuo

    1985-01-01

    This report presents the tensile strength behavior of steel fiber reinforced concrete (SFRC). In this experimental work, direct tensile test by using clamping grips is carried out for the prismatic mortar and concrete specimens reinforced with steel fibers. The volume content of steel fibers varies from 1.0 percent to 2.0 percent of the concrete volume. Test results for different fiber content, fiber length, and specimen size are presented. The results are compared with the results of indirec...

  6. A clinical comparative analysis of crush/clamp, stapler, and dissecting sealer hepatic transection methods1

    OpenAIRE

    Castaldo, Eric T.; Earl, T. Mark; Chari, Ravi S.; Gorden, D. Lee; Merchant, Nipun B; Wright, J. Kelly; Feurer, Irene D; Pinson, C Wright

    2008-01-01

    Introduction. Several methods for hepatic parenchymal division exist. The primary aim was to assess differences in postoperative bile leaks, operative blood loss, and margin status between three transection methods: crush/clamp (CC), stapler (SP), or dissecting sealer (DS). Methods. A single institution, retrospective cohort study was performed on data collected over a three-year period in patients undergoing elective liver resection using the CC, SP, or DS. Patients were excluded if multiple...

  7. Visual patch clamp recording of neurons in thick portions of the adult spinal cord

    DEFF Research Database (Denmark)

    Munch, Anders Sonne; Smith, Morten; Moldovan, Mihai; Perrier, Jean-Francois Marie

    2010-01-01

    enlargement of the spinal cord. With a conventional upright microscope in which the light condenser was carefully adjusted, we could visualize neurons present at the surface of the slice and record them with the whole-cell patch clamp technique. We show that neurons present in the middle of the preparation...... currents (IPSCs) remains constant. These preliminary data suggest that inhibitory and excitatory synaptic connections are balanced locally while excitation dominates long-range connections in the spinal cord....

  8. Study of hepatocellular function in the murine model following hepatic artery selective clamping

    OpenAIRE

    Tralhão, JG; Abrantes, AM; Gonçalves, AC; Hoti, E.; Laranjo, M; Martins, R.; Oliveiros, B.; Cardoso, D; Sarmento-Ribeiro, AB; Botelho, MF; Castro e Sousa, F

    2013-01-01

    PURPOSE: To investigate the impact of selective hepatic artery clamping (SHAC) in hepatocellular function. METHODS: Three groups of Wistar male rats were subjected to SHAC ischemia period of 60min: Group A continuous SHAC were subjected to SHAC ischemia period of 60min, Group B intermittent SHAC of 30min with 5min of reperfusion and Group C intermittent SHAC of 15min with 5min of reperfusion. Animals without SHAC were included-Group D. To evaluate hepatocellular function blood marker...

  9. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.

    OpenAIRE

    Vollrath, D; Davis, R.W.

    1987-01-01

    Excellent resolution of chromosomal DNA molecules from Saccharomyces cerevisiae, Candida albicans and Schizosaccharomyces pombe has been obtained using alternating contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The largest of these molecules is greater than 5 Mb in size and is resolved after 130 hours in a 0.6% agarose gel at a field strength of 1.3 V/cm and a switching interval of 1 hour. Separation of concatamers of phage lambda DNA reveals four regions of resolution...

  10. High Speed On-Off Valve Self-adapting Clamping System

    OpenAIRE

    Bing Wang

    2014-01-01

    The high-speed switch electromagnetic valve was a new type of element in the electro-hydraulic control system. The study aimed at the functional requirements and technical specifications of the turbine governor system for high speed on-off valve, designed the new Pulse Width Modulation high speed on-off valve. The high speed on-off valve could control fixture clamping force. Through the analysis of the working characteristics of the hydraulic actuator and h...

  11. Development of a computer interface for a clamp-on ultrasonic flow meter

    OpenAIRE

    Sundin, Peter

    2007-01-01

    The section for volume, flow and temperature at SP Technical Research Institute of Sweden performs measurements of volume, flow and temperature in liquids. Flow meters are best calibrated in its installation to take sources of error like installation effects and the medium into account. If this can be done without having to place measurement equipment inside the pipe it will mean several practical benefits. Since many years, clamp-on ultrasonic flow meters have been available on the market. B...

  12. Higher Accurate Estimation of Axial and Bending Stiffnesses of Plates Clamped by Bolts

    Science.gov (United States)

    Naruse, Tomohiro; Shibutani, Yoji

    Equivalent stiffness of clamped plates should be prescribed not only to evaluate the strength of bolted joints by the scheme of “joint diagram” but also to make structural analyses for practical structures with many bolted joints. We estimated the axial stiffness and bending stiffness of clamped plates by using Finite Element (FE) analyses while taking the contact condition on bearing surfaces and between the plates into account. The FE models were constructed for bolted joints tightened with M8, 10, 12 and 16 bolts and plate thicknesses of 3.2, 4.5, 6.0 and 9.0 mm, and the axial and bending compliances were precisely evaluated. These compliances of clamped plates were compared with those from VDI 2230 (2003) code, in which the equivalent conical compressive stress field in the plate has been assumed. The code gives larger axial stiffness for 11% and larger bending stiffness for 22%, and it cannot apply to the clamped plates with different thickness. Thus the code shall give lower bolt stress (unsafe estimation). We modified the vertical angle tangent, tanφ, of the equivalent conical by adding a term of the logarithm of thickness ratio t1/t2 and by fitting to the analysis results. The modified tanφ can estimate the axial compliance with the error from -1.5% to 6.8% and the bending compliance with the error from -6.5% to 10%. Furthermore, the modified tanφ can take the thickness difference into consideration.

  13. A helping clamp for thoracoscopic plication of eventration of the diaphragm

    OpenAIRE

    Reju Joseph Thomas; Ravi Kishore; Sundeep Kisku

    2011-01-01

    Background and Aim: It is difficult to suture an extremely thin and billowed up congenital eventration of the diaphragm thoracoscopically, without insufflation. Materials and Methods: The authors describe their technique using an intestinal clamp to control the redundant tissue and a feeding tube as a flexible knot pusher, to perform the thoracoscopic plication without risking hypercapnia. Results: A satisfactory result was obtained in all the four children. Conclusion: This is a useful adjun...

  14. A helping clamp for thoracoscopic plication of eventration of the diaphragm

    Directory of Open Access Journals (Sweden)

    Reju Joseph Thomas

    2011-01-01

    Full Text Available Background and Aim: It is difficult to suture an extremely thin and billowed up congenital eventration of the diaphragm thoracoscopically, without insufflation. Materials and Methods: The authors describe their technique using an intestinal clamp to control the redundant tissue and a feeding tube as a flexible knot pusher, to perform the thoracoscopic plication without risking hypercapnia. Results: A satisfactory result was obtained in all the four children. Conclusion: This is a useful adjunctive for thoracoscopic plication of diaphragmatic eventration.

  15. Prediction of numerical distortion after welding with various welding sequences and clampings

    OpenAIRE

    S. Kastelic; J. Medved; Mrvar, P.

    2010-01-01

    Welding simulation of a test cover for hydropower plant was made due to very large dimensions of the cover. The main aim was to predict distortion after welding in order to avoid machining the cover. Welding process was simulated with the Sysweld program to keep distortion in desired limits. Various welding sequences and clamping conditions were calculated to reduce the distortion. Calculation of microstructure constituents in virtual complex geometry of joints was also analyzed.

  16. Efficient Power Conversion in Common Active Clamp for Interleaved Dc-Dc Boost

    Directory of Open Access Journals (Sweden)

    V. Rathinavel Subramaniam

    2014-10-01

    Full Text Available This project presents a high-efficiency and high-step-up non isolated interleaved dc to dc converter with a common active-clamp circuit. In the presented converter, the coupled-inductor boost converters are interleaved. A boost converter is used to clamp the voltage stresses of all the switches in the interleaved converters, caused by the leakage inductances present in the practical coupled inductors, to a low voltage level. The leakage energies of the inter-leaved converters are collected in a clamp capacitor and recycled to the output by the clamp boost converter. The proposed converter achieves high efficiency because of the recycling of the leakage energies, reduction of the switch voltage stress, mitigation of the output diode is reverse recovery problem, and interleaving of the converters. In many applications, high-efficiency, high-voltage step-up dc–dc converters are required as an interface between the available low voltage sources and the output loads, which are operated at much higher voltages. Examples of such applications are as follows. Different distributed energy storage components such as batteries, fuel cells, and ultra capacitors are used in the power trains of hybrid electric vehicles (HEV, electric vehicles (EV, and fuel cell vehicles (FCV. In the present power train architectures of these vehicles, the voltage levels of the energy storage elements are usually low; whereas the motors of the vehicles are driven at much higher voltages. The telecom and the computer industry utilize the standard batteries, with low voltage levels, as a back-up power source. The dc–dc converter, used in this case, is required to boost the low-input voltage of the batteries to the high voltage of the dc bus. Another example is the automotive headlamps, using the high-intensity discharge lamp ballasts..

  17. A Color LED Driver Implemented by the Active Clamp Forward Converter

    OpenAIRE

    C. H. Chang; Cheng, H L; C. A. Cheng; E. C. Chang

    2013-01-01

    Because light emitting diodes (LEDs) have the advantages of dc working voltage, high luminescent efficiency, shortignition time, high reliability and pollution free, they have substituted for incandescent bulbs and fluorescent lampsgradually. In order to simplify circuit complexity, an active clamp forward converter with the sequential color display(SCD) control is proposed to drive red, green and blue (RGB) LED arrays. The proposed converter has zero-voltageswitching (ZVS) operations of both...

  18. Photoluminescence clamping with few excitons in a single-walled carbon nanotube

    OpenAIRE

    Xiao, Y. -F.; Nhan, T. Q.; Wilson, M. W. B.; Fraser, J. M.

    2009-01-01

    Single air-suspended carbon nanotubes (length 2 - 5 microns) exhibit high optical quantum efficiency (7 - 20%) for resonant pumping at low intensities. Under ultrafast excitation, the photoluminescence dramatically saturates for very low injected exciton numbers (2 to 6 excitons per pulse per SWCNT). This PL clamping is attributed to highly efficient exciton-exciton annihilation over micron length scales. Stochastic modeling of exciton dynamics and femtosecond excitation correlation spectrosc...

  19. What we talk about when we talk about capacitance measured with the voltage-clamp step method

    OpenAIRE

    Taylor, Adam L.

    2011-01-01

    Capacitance is a fundamental neuronal property. One common way to measure capacitance is to deliver a small voltage-clamp step that is long enough for the clamp current to come to steady state, and then to divide the integrated transient charge by the voltage-clamp step size. In an isopotential neuron, this method is known to measure the total cell capacitance. However, in a cell that is not isopotential, this measures only a fraction of the total capacitance. This has generally been thought ...

  20. Validation studies of seismic PRAs

    International Nuclear Information System (INIS)

    The validity and limitations of seismic probabilistic risk assessment methods are examined by determining how uncertainties due to inherent variability, modeling assumptions, and incomplete data effect risk estimates and inferences. The analysis was performed using the plant logic for the Limerick Generating Station for illustrative purposes. The role of uncertainties in seismic hazard analysis and component fragility modeling was assessed. Although the seismic hazard dominates core damage probability calculations, the component fragility modeling may be significant in seismic margin studies. (orig.)

  1. Seismic Disaster Reduction in China

    Institute of Scientific and Technical Information of China (English)

    Ministry of Construction

    2001-01-01

    @@ Great accomplishments have been made in seismic disaster reduction in China's engineering construction and city construction projects during the past decade (1990~2000). A new national map on the division of seismic intensity has been promulgated, and a series of anti-seismic standards and norms have been drafted or revised, which has further improved the country's technical code system on anti-seismic engineering measures.

  2. Seismic engineering: 1993--Volume 1

    International Nuclear Information System (INIS)

    Separate abstracts were prepared for the technical papers presented at the American Society of Mechanical Engineers 1993 Pressure Vessels and Piping Conference on July 25--29 in Denver, Colorado. Knowledge gained from seismic effect and method development for seismic analysis have seen continuing advancement in the past year. This volume contains fifteen papers dealing with seismic methods, analysis, and applications, nine papers dealing with piping seismic design and evaluation, and eight papers dealing with piping supports and components

  3. A capacitor-clamped inverter based torsional oscillation damping method for electromechanical drivetrains

    Directory of Open Access Journals (Sweden)

    Shagar Viknash

    2016-01-01

    Full Text Available A typical electromechanical drivetrain consists of an electric motor, connecting shafts and gears. Premature failures of these shafts and gears have been reported which are mainly due to fatigue caused by extreme loads and torsional oscillations. Overdesign and passive damping are the common approaches taken to increase the fatigue life. Nevertheless, they increase the system cost, weight and volume. Alternatively, active damping through advanced inverter control of the motor drive has been identified as a promising solution that does not require overdesign or alterations to the existing system. Even with the active damping control, oscillations propagate into the dc side of the power converter and subsequently to the upstream power bus. Generally, a large capacitor or an additional energy storage system is placed to suppress these oscillations. This paper proposes to use the clamping capacitors of the capacitor-clamped inverter as energy storage elements and thereby eliminate the need for a large dc side capacitor or an additional energy storage system. The efficacy of the proposed method has been verified with computer simulations. Simulation results show that the clamping capacitors are capable of containing torsional oscillations within the inverter without passing them to the upstream power bus.

  4. An ANN Based Capicitor Voltage Balancing Method For Neutral Point Clamped Multi-Level Inverter

    Directory of Open Access Journals (Sweden)

    P.J.S. Praveen,

    2015-11-01

    Full Text Available Multi-level inverters are became popular for usage in medium voltage, low voltage power applications due to flexibility in control and better performance characteristics in terms of harmonic regulation. Neutral point clamped are popular as they require less number of sources as their input when compared with their counter parts i.e. cascaded multi-level inverters and found to be reliable when compared with flying capacitor based multi-level inverters. But when neutral clamped technologies are used for generation of three-phase voltages, the capacitors that are connected at input side experiences imbalance in their voltages, this makes neutral point clamped multi-level inverters less reliable. In the proposed work an attempt is made to study circuits that balances the capacitor voltages and a scheme is investigated for balancing the capacitor voltages. Method proposed in [1] uses PID controller for balancing the capacitor voltages. In this project PI based control scheme and artificial neural network (ANN based control scheme for the front end circuit shown in [1] are designed for achieving balance among the capacitor voltages. The proposed control scheme is simulated with the help of Simpowersystems block set and neural network toolbox of MATLAB software for different load conditions. Results obtained from ANN based controller and PI controller are presented.

  5. Desktop Software for Patch-Clamp Raw Binary Data Conversion and Preprocessing

    Directory of Open Access Journals (Sweden)

    Ning Zhang

    2011-01-01

    Full Text Available Since raw data recorded by patch-clamp systems are always stored in binary format, electrophysiologists may experience difficulties with patch clamp data preprocessing especially when they want to analyze by custom-designed algorithms. In this study, we present desktop software, called PCDReader, which could be an effective and convenient solution for patch clamp data preprocessing for daily laboratory use. We designed a novel class module, called clsPulseData, to directly read the raw data along with the parameters recorded from HEKA instruments without any other program support. By a graphical user interface, raw binary data files can be converted into several kinds of ASCII text files for further analysis, with several preprocessing options. And the parameters can also be viewed, modified and exported into ASCII files by a user-friendly Explorer style window. The real-time data loading technique and optimized memory management programming makes PCDReader a fast and efficient tool. The compiled software along with the source code of the clsPulseData class module is freely available to academic and nonprofit users.

  6. A phage-encoded inhibitor of Escherichia coli DNA replication targets the DNA polymerase clamp loader.

    Science.gov (United States)

    Yano, Sho T; Rothman-Denes, Lucia B

    2011-03-01

    Coliphage N4 infection leads to shut-off of host DNA replication without inhibition of host transcription or translation. We report the identification and characterization of gp8, the N4 gene product responsible for this phenotype. N4 gp8 is an Escherichia coli bacteriostatic inhibitor that colocalizes with the E. coli replisome in a replication-dependent manner. Gp8 was purified and observed to cross-link to complexes containing the replicative DNA polymerase, DNAP III, in vivo. Purified gp8 inhibits DNA polymerization by DNA polymerase III holoenzyme in vitro by interfering with polymerase processivity. Gp8 specifically inhibits the clamp-loading activity of DNAP III by targeting the delta subunit of the DNAP III clamp loader; E. coli mutations conferring gp8 resistance were identified in the holA gene, encoding delta. Delta and gp8 interact in vitro; no interaction was detected between gp8 inactive mutants and wild-type delta or between delta gp8-resistant mutants and wild-type gp8. Therefore, this work identifies the DNAP III clamp loader as a new target for inhibition of bacterial growth. Finally, we show that gp8 is not essential in N4 development under laboratory conditions, but its activity contributes to phage yield. PMID:21205014

  7. Molecular jamming--the cystine slipknot mechanical clamp in all-atom simulations.

    Science.gov (United States)

    Pepłowski, Lukasz; Sikora, Mateusz; Nowak, Wiesław; Cieplak, Marek

    2011-02-28

    A recent survey of 17 134 proteins has identified a new class of proteins which are expected to yield stretching induced force peaks in the range of 1 nN. Such high force peaks should be due to forcing of a slip-loop through a cystine ring, i.e., by generating a cystine slipknot. The survey has been performed in a simple coarse grained model. Here, we perform all-atom steered molecular dynamics simulations on 15 cystine knot proteins and determine their resistance to stretching. In agreement with previous studies within a coarse grained structure based model, the level of resistance is found to be substantially higher than in proteins in which the mechanical clamp operates through shear. The large stretching forces arise through formation of the cystine slipknot mechanical clamp and the resulting steric jamming. We elucidate the workings of such a clamp in an atomic detail. We also study the behavior of five top strength proteins with the shear-based mechanostability in which no jamming is involved. We show that in the atomic model, the jamming state is relieved by moving one amino acid at a time and there is a choice in the selection of the amino acid that advances the first. In contrast, the coarse grained model also allows for a simultaneous passage of two amino acids. PMID:21361557

  8. Molecular jamming—The cystine slipknot mechanical clamp in all-atom simulations

    Science.gov (United States)

    Pepłowski, Łukasz; Sikora, Mateusz; Nowak, Wiesław; Cieplak, Marek

    2011-02-01

    A recent survey of 17 134 proteins has identified a new class of proteins which are expected to yield stretching induced force peaks in the range of 1 nN. Such high force peaks should be due to forcing of a slip-loop through a cystine ring, i.e., by generating a cystine slipknot. The survey has been performed in a simple coarse grained model. Here, we perform all-atom steered molecular dynamics simulations on 15 cystine knot proteins and determine their resistance to stretching. In agreement with previous studies within a coarse grained structure based model, the level of resistance is found to be substantially higher than in proteins in which the mechanical clamp operates through shear. The large stretching forces arise through formation of the cystine slipknot mechanical clamp and the resulting steric jamming. We elucidate the workings of such a clamp in an atomic detail. We also study the behavior of five top strength proteins with the shear-based mechanostability in which no jamming is involved. We show that in the atomic model, the jamming state is relieved by moving one amino acid at a time and there is a choice in the selection of the amino acid that advances the first. In contrast, the coarse grained model also allows for a simultaneous passage of two amino acids.

  9. Is Near-Infrared Spectroscopy a Reliable Method to Evaluate Clamping Ischemia during Carotid Surgery?

    Directory of Open Access Journals (Sweden)

    Luciano Pedrini

    2012-01-01

    Full Text Available Guidelines do not include cerebral oximetry among monitoring for carotid endarterectomy (CEA. The purpose of this study was to evaluate the reliability of near-infrared spectroscopy (NIRS in the detection of clamping ischemia and in the prevention of clamping-related neurologic deficits using, as a cutoff for shunting, a 20% regional cerebral oxygen saturation (rSO2 decrease if persistent more than 4 minutes, otherwise a 25% rSO2 decrease. Bilateral rSO2 was monitored continuously in patients undergoing CEA under general anesthesia (GA. Data was recorded after clamping, declamping, during shunting and lowest values achieved. Preoperative neurologic, CT-scan, and vascular lesions were recorded. We reviewed 473 cases: 305 males (64.5% mean age 73.3±7.3. Three patients presented transient ischemic deficits at awakening, no perioperative stroke or death; 41 (8.7% required shunting: 30 based on the initial rSO2 value and 11 due to a decrease during surgery. Using the ROC curve analysis we found, for a >25% reduction from baseline value, a sensitivity of 100% and a specificity of 90.6%. Reliability, PPV, and NPV were 95.38%, 9%, and 100%, respectively. In conclusion, this study indicates the potential reliability of NIRS monitoring during CEA under GA, using a cutoff of 25% or a cutoff of 20% for prolonged hypoperfusion.

  10. Three Level Diode Clamped Inverter Fed Induction Motor with Dtc-Svm

    Directory of Open Access Journals (Sweden)

    V. Ranjith Kumar

    2013-09-01

    Full Text Available This project presents a three-level diode-clamped inverter feeding an asynchronous motor drive with direct torque control (DTC. The control method is based on DTC operating principles. The stator voltage vector reference is computed from the stator flux and torque errors imposed by the flux and torque controllers. This voltage reference is then generated using a diode-clamped inverter, where each phase of the inverter can be implemented using a dc source, which would be available from fuel cells, batteries, or ultra capacitors. This inverter provides nearly sinusoidal voltages with very low distortion, even without filtering, using fewer switching devices. In addition, the multilevel inverter can generate a high and fixed switching frequency output voltage with fewer switching losses, since only the small power cells of the inverter operate at a high switching rate. Therefore, a high performance and also efficient torque and flux controllers are obtained, enabling a DTC solution for multilevel-inverter-powered motor drives. The Pulse Width modulation technique for an inverter permits to obtain three phase system voltages, which can be applied to the controlled output. Space Vector Modulation (SVPWM principle differs from other PWM processes in the fact that all three drive signals for the inverter will be created simultaneously. The implementation of SVPWM process in digital systems necessitates less operation time and also less program memory. This project uses SVPWM technique for generation of pulses for three-level diode clamped inverter. Simulation is carried out on MATLAB-Simulink software.

  11. Migration of seismic data

    International Nuclear Information System (INIS)

    Prospecting for oil and gas resources poses the problem of determining the geological structure of the earth's crust from indirect measurements. Seismic migration is an acoustic image reconstruction technique based on the inversion of the scalar wave equation. Extensive computation is necessary before reliable information can be extracted form large sets of recorded data. In this paper a collection of open-quotes industrialclose quotes migration techniques, each giving rise to a data parallel algorithm, is outlined. Computer simulations on synthetic seismic data illustrate the problem and the approach

  12. Induced Seismicity Monitoring System

    Science.gov (United States)

    Taylor, S. R.; Jarpe, S.; Harben, P.

    2014-12-01

    There are many seismological aspects associated with monitoring of permanent storage of carbon dioxide (CO2) in geologic formations. Many of these include monitoring underground gas migration through detailed tomographic studies of rock properties, integrity of the cap rock and micro seismicity with time. These types of studies require expensive deployments of surface and borehole sensors in the vicinity of the CO2 injection wells. Another problem that may exist in CO2 sequestration fields is the potential for damaging induced seismicity associated with fluid injection into the geologic reservoir. Seismic hazard monitoring in CO2 sequestration fields requires a seismic network over a spatially larger region possibly having stations in remote settings. Expensive observatory-grade seismic systems are not necessary for seismic hazard deployments or small-scale tomographic studies. Hazard monitoring requires accurate location of induced seismicity to magnitude levels only slightly less than that which can be felt at the surface (e.g. magnitude 1), and the frequencies of interest for tomographic analysis are ~1 Hz and greater. We have developed a seismo/acoustic smart sensor system that can achieve the goals necessary for induced seismicity monitoring in CO2 sequestration fields. The unit is inexpensive, lightweight, easy to deploy, can operate remotely under harsh conditions and features 9 channels of recording (currently 3C 4.5 Hz geophone, MEMS accelerometer and microphone). An on-board processor allows for satellite transmission of parameter data to a processing center. Continuous or event-detected data is kept on two removable flash SD cards of up to 64+ Gbytes each. If available, data can be transmitted via cell phone modem or picked up via site visits. Low-power consumption allows for autonomous operation using only a 10 watt solar panel and a gel-cell battery. The system has been successfully tested for long-term (> 6 months) remote operations over a wide range

  13. Facies characterization based on physical properties from downhole logging for the sediment record of Lake Van, Turkey

    Science.gov (United States)

    Baumgarten, H.; Wonik, T.; Kwiecien, O.

    2014-11-01

    Lake Van (Turkey) is the 4th largest terminal lake in the world and is located at a key position for climatic reconstruction. The ICDP project 'PALEOVAN' is a deep-drilling campaign initiated in the summer of 2010 to enhance the understanding of paleoclimatic and paleoenvironmental conditions in the Middle East for a period of 550,000 years. Multiple coring of two sites (Northern Basin and Ahlat Ridge) at a water depth of up to 360 m has been performed. The sedimentary record is mainly composed of clayey silts and tephra deposits that were supplied by four volcanic sources: 1) the Süphan volcano, located on the northern shore, 2) the Nemrut volcano, 15 km west of the westernshore, 3) the Incekaya volcano, on the southwestern shore and 4) intralake eruptive centers. The dominant chemical composition of the volcanic sources is known from studies of land deposits. High-quality downhole logs have been acquired from both sites. To construct a continuous lithological profile, 180 m of downhole logging data from the Ahlat Ridge have been analyzed by cluster analysis. To improve the differentiation of the sediments, two elemental intensity profiles from x-ray fluorescence core-scanning (calcium and zirconium) performed on the composite profile core material were added to the cluster analysis. Five cluster units were derived and transformed into three tephra and two clayey silt units. To compare the two clayey silt units with the composite profile from the visual core description (VCD) that showed 15 lithological units, the composite profile was classified into two major groups of lacustrine sediments: banded clayey silts (interpreted as glacial deposits) and laminated clayey silts (interpreted as interglacial deposits). Despite this simplification, no correlation between the two clayey silt units derived from cluster analysis and the banded and laminated clayey silts could be found. The following reasons are proposed: (a) the comparability of the datasets was limited by

  14. Opportunities of downhole dielectric heating in Venezuela : three case studies involving medium, heavy, and extra-heavy crude oil reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Ovalles, C.; Fonseca, A.; Lara, A.; Alvarado, V.; Urrechaga, K.; Ranson, A.; Mendoza, H. [Petroleos de Venezuela SA - Intevep, Caracas (Venezuela)

    2002-07-01

    This paper presented some potential applications for electromagnetic radiation, radiofrequency (RF) and microwaves (MW) for enhanced oil recovery (EOR). A study was conducted to examine how RF and MW can be used for downhole dielectric heating. This was accomplished by modelling reservoir dielectric heating through the use of the Lambert's equation coupled to a commercial simulator. The model was validated using crude oil and sand cores from 3 different reservoirs of medium, heavy and extra-heavy crude oils from the Lake Maracaibo reservoir in the Orinoco Basin in Venezuela. A numerical simulation demonstrated a significant acceleration in the oil production due to RF and MW heating. This was attributed to reduced viscosity of the crude oil plus the favourable water-to-oil permeabilities. It was concluded that, according to numerical simulations, there is a high potential for dielectric heating technology for medium, heavy and extra-heavy crude oils. 33 refs., 3 tabs., 13 figs.

  15. Leak finder : new twist on fibre optics promises well-operators an ear for downhole gas leaks

    Energy Technology Data Exchange (ETDEWEB)

    Mahony, J.

    2010-04-15

    Alberta's Energy Resources Conservation Board (ERCB) requires that well operators report downhole gas leaks. Leaks fall into two groups, notably gas migration, where gas works its way to surface, usually outside the wellbore, through holes in the concrete around the casing; and surface casing vent flow, where gas moves up the wellbore, usually between the production casing and surface casing. The ERCB gauges the seriousness of the leak through a formula that considers well depth, gas pressure and flow rate. If deemed serious, a leak must be repaired within 90 days. If deemed non-serious, the operator need only monitor the casing vent flow for changes in pressure, volume or both. The greatest challenge is finding the leak. Until recently, the tools for the job were a microphone on a wireline. However, a new method that provides a faster, more accurate way of finding downhole gas leaks has been developed by Alberta-based HiFi Engineering. The method uses fibre optics as a sensor and as a transmission medium. The fibre is treated to effectively transform it into an acoustic sensor. After treatment, the fibre itself becomes the microphone and is highly sensitive to acoustics. The fibre can move data at the speed of light and can also sense very faint sounds in the wellbore, making it useful for tracing casing vent flows or gas migration through the noise produced. HiFi's soon to be commercialized fibre optic technology has been extensively tested in the laboratory. The distributed fibre optic acoustic sensors have also been tested on 4 Cenovus natural gas wells in Alberta. The signal-to-noise ratio is key to the usefulness of any sound-detection device. The sensitivity of the tool increases as the signal-to-noise ratio increases. 1 refs., 3 figs.

  16. Experimental determination of optimal clamping torque for AB-PEM Fuel cell

    Directory of Open Access Journals (Sweden)

    Noor Ul Hassan

    2016-04-01

    Full Text Available Polymer electrolyte Membrane (PEM fuel cell is an electrochemical device producing electricity by the reaction of hydrogen and oxygen without combustion. PEM fuel cell stack is provided with an appropriate clamping torque to prevent leakage of reactant gases and to minimize the contact resistance between gas diffusion media (GDL and bipolar plates. GDL porous structure and gas permeability is directly affected by the compaction pressure which, consequently, drastically change the fuel cell performance. Various efforts were made to determine the optimal compaction pressure and pressure distributions through simulations and experimentation. Lower compaction pressure results in increase of contact resistance and also chances of leakage. On the other hand, higher compaction pressure decreases the contact resistance but also narrows down the diffusion path for mass transfer from gas channels to the catalyst layers, consequently, lowering cell performance. The optimal cell performance is related to the gasket thickness and compression pressure on GDL. Every stack has a unique assembly pressure due to differences in fuel cell components material and stack design. Therefore, there is still need to determine the optimal torque value for getting the optimal cell performance. This study has been carried out in continuation of deve­lopment of Air breathing PEM fuel cell for small Unmanned Aerial Vehicle (UAV application. Compaction pressure at minimum contact resistance was determined and clamping torque value was calcu­la­ted accordingly. Single cell performance tests were performed at five different clamping torque values i.e 0.5, 1.0, 1.5, 2.0 and 2.5 N m, for achieving optimal cell per­formance. Clamping pressure distribution tests were also performed at these torque values to verify uniform pressure distribution at optimal torque value. Experimental and theoretical results were compared for making inferences about optimal cell perfor­man­ce. A

  17. In vivo measurements of whole body (WB) and skeletal muscle glucose metabolism under basal and euglycemic insulin clamp (Clamp) by combined PET and stable isotope (SI) tracer studies

    International Nuclear Information System (INIS)

    Aim/Background: Primed-constant infusion of SI labeled tracers is a classic technique for studying metabolism at the WB level, however, this procedure provides no information about the metabolism of specific tissues. In contrast PET provides primarily tissue specific data. In this study, we combined PET with SI techniques to measure glucose metabolism in WB and lower limb skeletal muscle (LLM) of humans under Basal and Clamp conditions. Methods and Materials: Four healthy volunteers (73.0 ± 6.0 kg, mean ± sem) were studied. After fasting overnight, each subject was injected with 10 mCi of 18FDG and serial 1.0 min. PET images of the mid-thigh region were acquired over 90 min. Arterial blood samples were collected in parallel. Glucose metabolic rate (GM) was calculated with a 3-compartment / 4 rate constant model; LC assumed to be 1.0. A primed constant infusion of [6,6, 2H2]glucose was performed in parallel with the PET measurements. On another day, the PET and SI measurements were repeated under clamp conditions. All results are expressed as mean ± sem. Results: The glucose kinetics in whole body and in low limb skeletal muscles are shown. Under in vivo conditions, Clamp caused: 1) a 10.2 ± 2.3 fold increase in GM by LLM but only a 4.7 ± 0.4 fold increase in GM by MB. 2) Increased contribution of LLM to WB GM, indicating that LLM GM is more sensitive to insulin compared with anterior LLM (extensors). Discussion: The study demonstrated the unique advantages of using PET to study substrate metabolism in specific tissues in human subjects: i) It is less invasive than the conventional A-V difference and muscle biopsy method. ii) It provides a more detailed picture of substrate metabolism in different parts of the muscle in the same limb, as compared to one spot muscle biopsy. Data in demonstrated that GM in posterior LLM is more sensitive to insulin than that in anterior LLM. iii) It can detect substrate metabolism in deep muscles which cannot be reached by biopsy

  18. Multichannel Analysis of Surface Waves and Down-Hole Tests in the Archeological "Palatine Hill" Area (Rome, Italy): Evaluation and Influence of 2D Effects on the Shear Wave Velocity

    Science.gov (United States)

    Di Fiore, V.; Cavuoto, G.; Tarallo, D.; Punzo, M.; Evangelista, L.

    2016-05-01

    A joint analysis of down-hole (DH) and multichannel analysis of surface waves (MASW) measurements offers a complete evaluation of shear wave velocity profiles, especially for sites where a strong lateral variability is expected, such as archeological sites. In this complex stratigraphic setting, the high "subsoil anisotropy" (i.e., sharp lithological changes due to the presence of anthropogenic backfill deposits and/or buried man-made structures) implies a different role for DH and MASW tests. This paper discusses some results of a broad experimental program conducted on the Palatine Hill, one of the most ancient areas of the city of Rome (Italy). The experiments were part of a project on seismic microzoning and consisted of 20 MASW and 11 DH tests. The main objective of this study was to examine the difficulties related to the interpretation of the DH and MASW tests and the reliability limits inherent in the application of the noninvasive method in complex stratigraphic settings. As is well known, DH tests provide good determinations of shear wave velocities (Vs) for different lithologies and man-made materials, whereas MASW tests provide average values for the subsoil volume investigated. The data obtained from each method with blind tests were compared and were correlated to site-specific subsurface conditions, including lateral variability. Differences between punctual (DH) and global (MASW) Vs measurements are discussed, quantifying the errors by synthetic comparison and by site response analyses. This study demonstrates that, for archeological sites, VS profiles obtained from the DH and MASW methods differ by more than 15 %. However, the local site effect showed comparable results in terms of natural frequencies, whereas the resolution of the inverted shear wave velocity was influenced by the fundamental mode of propagation.

  19. Simulation Based Performance analysis of Active Clamp DHB ZVZCS Bidirectional DC-DC converter for Low Power Applications

    Directory of Open Access Journals (Sweden)

    V.V.Subrahmanya Kumar Bhajana

    2012-06-01

    Full Text Available A novel active clamp dual half bridge DC/DC converter with bidirectional power flow is proposed in this paper and comparative analysis between active clamp DHB ZVZCS and ZVS-ZCS bidirectional DC-DC converter topologies is also presented. By adding active clamping circuits to both bridges, zero voltage and zero current switching are achieved to improve the performance of the bidirectional DC/DC converter. The principle of operation is analyzed and simulated. With the proposed active clamp ZVZCS concept, the MATLAB simulation results of the applications of the fuel cell and battery have been obtained and compared with those of ZVS-ZCS bidirectional converter. The simulation results of proposed converter is compared with the ZVS-ZCS bidirectional DC-DC converter, efficiency, switching losses are the key parameters compared.

  20. Robotic clamp and index tool and method for their use in repairing certain elements with in a nuclear reactor

    International Nuclear Information System (INIS)

    This patent describes a clamp-and-index tool for use in the replacement of old split-pin assemblies of a guide tube of a nuclear reactor by new split-pin assemblies, the old split-pin assemblies and the guide tube being radioactive and the clamp-and-index tool to operate under a pool of water. The tool includes means, to be connected to the guide tube, for suspending the guide tube in a first position in which the tube is to be processed to effect the replacement. The tool also includes a means, connected to the suspending means, for rotating the guide tube to a second position in which the tube is to be processed to effect the replacement and clamping means, connected to the suspending means and to be connected to the guide tube, for selectively clamping the guide tube in the first or second position

  1. Structure-function analysis of the C-clamp of TCF/Pangolin in Wnt/ß-catenin signaling.

    Directory of Open Access Journals (Sweden)

    Aditi J Ravindranath

    Full Text Available The evolutionarily conserved Wnt/ß-catenin (Wnt/ß-cat pathway plays an important role in animal development in metazoans. Many Wnt targets are regulated by members of the TCF/LEF1 (TCF family of transcription factors. All TCFs contain a High Mobility Group (HMG domain that bind specific DNA sequences. Invertebrate TCFs and some vertebrate TCF isoforms also contain another domain, called the C-clamp, which allows TCFs to recognize an additional DNA motif known as the Helper site. While the C-clamp has been shown to be important for regulating several Wnt reporter genes in cell culture, its physiological role in regulating Wnt targets is less clear. In addition, little is known about this domain, except that two of the four conserved cysteines are functionally important. Here, we carried out a systematic mutagenesis and functional analysis of the C-clamp from the Drosophila TCF/Pangolin (TCF/Pan protein. We found that the C-clamp is a zinc-binding domain that is sufficient for binding to the Helper site. In addition to this DNA-binding activity, the C-clamp also inhibits the HMG domain from binding its cognate DNA site. Point mutations were identified that specifically affected DNA-binding or reduced the inhibitory effect. These mutants were characterized in TCF/Pan rescue assays. The specific DNA-binding activity of the C-clamp was essential for TCF/Pan function in cell culture and in patterning the embryonic epidermis of Drosophila, demonstrating the importance of this C-clamp activity in regulating Wnt target gene expression. In contrast, the inhibitory mutation had a subtle effect in cell culture and no effect on TCF/Pan activity in embryos. These results provide important information about the functional domains of the C-clamp, and highlight its importance for Wnt/ß-cat signaling in Drosophila.

  2. High Voltage Seismic Generator

    Science.gov (United States)

    Bogacz, Adrian; Pala, Damian; Knafel, Marcin

    2015-04-01

    This contribution describes the preliminary result of annual cooperation of three student research groups from AGH UST in Krakow, Poland. The aim of this cooperation was to develop and construct a high voltage seismic wave generator. Constructed device uses a high-energy electrical discharge to generate seismic wave in ground. This type of device can be applied in several different methods of seismic measurement, but because of its limited power it is mainly dedicated for engineering geophysics. The source operates on a basic physical principles. The energy is stored in capacitor bank, which is charged by two stage low to high voltage converter. Stored energy is then released in very short time through high voltage thyristor in spark gap. The whole appliance is powered from li-ion battery and controlled by ATmega microcontroller. It is possible to construct larger and more powerful device. In this contribution the structure of device with technical specifications is resented. As a part of the investigation the prototype was built and series of experiments conducted. System parameter was measured, on this basis specification of elements for the final device were chosen. First stage of the project was successful. It was possible to efficiently generate seismic waves with constructed device. Then the field test was conducted. Spark gap wasplaced in shallowborehole(0.5 m) filled with salt water. Geophones were placed on the ground in straight line. The comparison of signal registered with hammer source and sparker source was made. The results of the test measurements are presented and discussed. Analysis of the collected data shows that characteristic of generated seismic signal is very promising, thus confirms possibility of practical application of the new high voltage generator. The biggest advantage of presented device after signal characteristics is its size which is 0.5 x 0.25 x 0.2 m and weight approximately 7 kg. This features with small li-ion battery makes

  3. Romanian seismic network

    International Nuclear Information System (INIS)

    The research in the field of seismology in Romania is mainly carried out by the National Institute for Earth Physics (NIEP). The NIEP activities are mainly concerned with the fundamental research financed by research contracts from public sources and the maintenance and operation of the Romanian seismic network. A three stage seismic network is now operating under NIEP, designed mainly to monitor the Vrancea seismic region in a magnitude range from microearthquakes to strong events: - network of 18 short-period seismometers (S13); - Teledyne Geotech Instruments (Texas); - network of 7 stations with local digital recording (PCM-5000) on magnetic tape, made up of, S13 geophone (T=2 s) on vertical component and SH1 geophone (T=5 s) on horizontal components; - network of 28 SMA-1 accelerometers and 30 digital accelerometers (Kinemetrics - K2) installed in the free field conditions in the framework of the joint German-Romanian cooperation program (CRC); the K2 instruments cover a magnitude range from 1.4 to 8.0. Since 1994, MLR (Muntele Rosu) station has become part of the GEOFON network and was provided with high performance broad band instruments. At Bucharest and Timisoara data centers, an automated and networked seismological system performs the on-line digital acquisition and processing of the telemetered data. Automatic processing includes discrimination between local and distant seismic events, earthquake location and magnitude computation, and source parameter determination for local earthquakes. The results are rapidly distributed via Internet, to several seismological services in Europe and USA, to be used in the association/confirmation procedures. Plans for new developments of the network include the upgrade from analog to digital telemetry and new stations for monitoring local seismicity. (authors)

  4. Seismic operations and farmers' rights

    International Nuclear Information System (INIS)

    The specific rights of farmers in Alberta regarding seismic operations on their land are described. A seismic operator can enter onto a property only after obtaining approval from the owner and occupant of the land. It is entirely up to the farmer to negotiate the terms of the contract. This document summarizes the chronology of a seismic survey and describes the steps required to conduct seismic tests on titled land, leased land and on leased road allowance. Also discussed are the implications of seismic operations on water wells and property damage. The required distances of various seismic operations, and the requirements for hole abandonment, cleanup and compensation are laid out. Special considerations for 3-D seismic surveys are also addressed

  5. Intermediate depth seismicity - a reflection seismic approach

    Science.gov (United States)

    Haberland, C.; Rietbrock, A.

    2004-12-01

    During subduction the descending oceanic lithosphere is subject to metamorphic reactions, some of them associated with the release of fluids. It is now widely accepted, that these reactions and associated dehydration processes are directly related with the generation of intermediate depth earthquakes (dehydration embrittlement). However, the structure of the layered oceanic plate at depth and the location of the earthquakes relative to structural units of the subducting plate (sources within the oceanic crust and/or in the upper oceanic mantle lithosphere?) are still not resolved yet. This is in mainly due to the fact that the observational resolution needed to address these topics (in the range of only a few kilometers) is hardly achieved in field experiments and related studies. Here we study the wavefields of intermediate depth earthquakes typically observed by temporary networks in order to assess their high-resolution potential in resolving structure of the down going slab and locus of seismicity. In particular we study whether the subducted oceanic Moho can be detected by the analysis of secondary phases of local earthquakes (near vertical reflection). Due to the irregular geometry of sources and receivers we apply an imaging technique similar to diffraction stack migration. The method is tested using synthetic data both based on 2-D finite difference simulations and 3-D kinematic ray tracing. The accuracy of the hypocenter location and onset times crucial for the successful application of stacking techniques (coherency) was achieved by the use of relatively relocated intermediate depth seismicity. Additionally, we simulate the propagation of the wavefields at larger distance (wide angle) indicating the development of guided waves traveling in the low-velocity waveguide associated with the modeled oceanic crust. We also present application on local earthquake data from the South American subduction zone.

  6. Seismic and Gravity Investigations of the Western Espanola Basin, Rio Grande Rift, New Mexico

    Science.gov (United States)

    Braile, L. W.; Coldren, B. G.; Baca, A.; Fontana, J.; Olheiser, M.; Ziff, M.; Keske, A.; Rhode, A.; Martin-Short, R.; Allen, W.; Denton, K. M.; Harper, C.; Baldridge, W.; Biehler, S.; Ferguson, J. F.; McPhee, D.; Snelson, C. M.

    2013-12-01

    The SAGE (Summer of Applied Geophysical Experience) program collected new seismic, gravity, electromagnetic and down-hole temperature data in 2013 in the western Espanola basin of the Rio Grande rift area of northern New Mexico. The location, about 25 km NW of Santa Fe, has been identified as a potential geothermal resources area based on relatively high temperature gradients in drill holes. The SAGE 2013 data collection was part of an integrated geophysical study of the area initiated in 2011. Seismic data consisted of a 4.8 km W to E profile (120 three-component stations in four overlapping deployments, 20 m station spacing, using a Vibroseis source - 20 m spacing for reflection VPs; 800 m spacing for refraction VPs) with both refraction and CMP reflection coverage. About 55,000 seismograms were recorded. The surface conditions (dry unconsolidated sediments) increased surface wave energy and limited the signal-to-noise level of the refraction and reflection arrivals. Utilizing longer source-receiver offsets improved the shot-gather record sections by emphasizing wider angle reflections which are very strong and coherent. The refraction data were modeled with first arrival travel time methods. The reflection data were processed to produce a CMP stacked record section. Strong reflectors from basin-filling sedimentary rocks (mostly Tertiary in age) are visible above reflections from a thin section of Paleozoic rocks and the basement. The lower reflections have an apparent dip to the west of about 12 degrees. Eighty-one new gravity measurements (detailed data at 200 m spacing along the seismic profile, and regional stations) were collected and combined with existing regional data for modeling. Interpretation of the seismic and gravity data was aided by refraction velocities, the existence of a nearby regional seismic reflection profile from industry, and lithologies and well-logs from a deep well. The sedimentary basin interpreted from the seismic and gravity data

  7. MEASUREMENT OF COMPRESSIONAL-WAVE SEISMIC VELOCITIES IN 29 WELLS AT THE HANFORD SITE

    Energy Technology Data Exchange (ETDEWEB)

    PETERSON SW

    2010-10-08

    Check shot seismic velocity surveys were collected in 100 B/C, 200 East, 200-PO-1 Operational Unit (OU), and the Gable Gap areas in order to provide time-depth correlation information to aid the interpretation of existing seismic reflection data acquired at the Hanford Site (Figure 1). This report details results from 5 wells surveyed in fiscal year (FY) 2008, 7 wells in FY 2009, and 17 wells in FY 2010 and provides summary compressional-wave seismic velocity information to help guide future seismic survey design as well as improve current interpretations of the seismic data (SSC 1979/1980; SGW-39675; SGW-43746). Augmenting the check shot database are four surveys acquired in 2007 in support of the Bechtel National, Inc. Waste Treatment Plant construction design (PNNL-16559, PNNL-16652), and check shot surveys in three wells to support seismic testing in the 200 West Area (Waddell et al., 1999). Additional sonic logging was conducted during the late 1970s and early 1980s as part of the Basalt Waste Isolation Program (BWIP) (SSC 1979/1980) and check shot/sonic surveys as part of the safety report for the Skagit/Hanford Nuclear project (RDH/10-AMCP-0164). Check shot surveys are used to obtain an in situ measure of compressional-wave seismic velocity for sediment and rock in the vicinity of the well point, and provide the seismic-wave travel time to geologic horizons of interest. The check shot method deploys a downhole seismic receiver (geophone) to record the arrival of seismic waves generated by a source at the ground surface. The travel time of the first arriving seismic-wave is determined and used to create a time-depth function to correlate encountered geologic intervals with the seismic data. This critical tie with the underlying geology improves the interpretation of seismic reflection profile information. Fieldwork for this investigation was conducted by in house staff during the weeks of September 22, 2008 for 5 wells in the 200 East Area (Figure 2); June 1

  8. The Analysis of Clamping Mechanism Theory and the Application Design of Tobacco Leaves'Clamping Tool%夹紧机构的理论分析及烘烤烟叶夹的应用设计

    Institute of Scientific and Technical Information of China (English)

    徐远龙; 孙有祥

    2011-01-01

    In view of the complicated manual skill of the tobacco leaf weaving, the study and design of a kind tool clamping tobacco leaf is proposed by our actual investigation; According to the predetermined function demand and the available clamping mechanism, the principle of the clamping tool is studied out, the structural design scheme of the clamping tool is worked out and the clamping tool is made by the theory analysis of mechanism design and the study of clamping mechanism. The clamping tool tested by the surveyor is good in use, which is welcome to use by the peasants and the economy benefit of the market can be achieved highly.%通过对烟叶烘烤生产工艺的实际调研,针对手工繁杂的编烟技术,提出了研究设计一种烘烤烟叶夹持工具;针对烘烤烟叶夹的预定功能要求,参考现有的实用夹紧机构,通过对机械产品机构设计的理论分析和夹紧机构的力学研究,确定了夹紧烟叶的力学原理,提出了烘烤烟叶夹的结构设计方案,设计制造出烘烤烟叶夹;该烘烤烟叶夹通过了产品的质量检测,使用效果良好,烟农用户普遍接受使用,预计有较高的市场经济效益.

  9. Clamping end-tidal carbon dioxide during graded exercise with control of inspired oxygen.

    Science.gov (United States)

    Farra, Saro D; Kessler, Cathie; Duffin, James; Wells, Greg D; Jacobs, Ira

    2016-09-01

    Exercise- and hypoxia-induced hyperventilation decreases the partial pressure of end-tidal carbon dioxide (PETCO2), which in turn exerts many physiological effects. Several breathing circuits that control PETCO2 have been previously described, but their designs are not satisfactory for exercise studies where changes in inspired oxygen (FIO2) may be desired. This study is the first report of a breathing system that can maintain PETCO2 constant within a single session of graded submaximal exercise and graded hypoxia. Thirteen fit and healthy subjects completed two bouts of exercise consisting of three 3min stages on a cycle ergometer with increasing exercise intensity in normoxia (Part A; 142±14, 167±14, 192±14W) or with decreasing FIO2 at a constant exercise intensity (Part B; 21, 18, and 14%). One bout was a control (CON) where PETCO2 was not manipulated, while during the other bout the investigator clamped PETCO2 within 2mmHg (CO2Clamp) using sequential gas delivery (SGD). During the final 30s of each exercise stage during CO2Clamp, PETCO2 was successfully maintained in Part A (43±4, 44±4, 44±3mmHg; P=0.44) and Part B (45±3, 46±3, 45±3mmHg; P=0.68) despite the increases in ventilation due to exercise. These findings demonstrate that this SGD circuit can be used to maintain isocapania in exercising humans during progressively increasing exercise intensities and changing FIO2. PMID:27236039

  10. Comparison of seismic sources for shallow seismic: sledgehammer and pyrotechnics

    Science.gov (United States)

    Brom, Aleksander; Stan-Kłeczek, Iwona

    2015-10-01

    The pyrotechnic materials are one of the types of the explosives materials which produce thermal, luminous or sound effects, gas, smoke and their combination as a result of a self-sustaining chemical reaction. Therefore, pyrotechnics can be used as a seismic source that is designed to release accumulated energy in a form of seismic wave recorded by tremor sensors (geophones) after its passage through the rock mass. The aim of this paper was to determine the utility of pyrotechnics for shallow seismic engineering. The work presented comparing the conventional method of seismic wave excitation for seismic refraction method like plate and hammer and activating of firecrackers on the surface. The energy released by various sources and frequency spectra was compared for the two types of sources. The obtained results did not determine which sources gave the better results but showed very interesting aspects of using pyrotechnics in seismic measurements for example the use of pyrotechnic materials in MASW.

  11. Comparison of seismic sources for shallow seismic: sledgehammer and pyrotechnics

    Directory of Open Access Journals (Sweden)

    Brom Aleksander

    2015-10-01

    Full Text Available The pyrotechnic materials are one of the types of the explosives materials which produce thermal, luminous or sound effects, gas, smoke and their combination as a result of a self-sustaining chemical reaction. Therefore, pyrotechnics can be used as a seismic source that is designed to release accumulated energy in a form of seismic wave recorded by tremor sensors (geophones after its passage through the rock mass. The aim of this paper was to determine the utility of pyrotechnics for shallow seismic engineering. The work presented comparing the conventional method of seismic wave excitation for seismic refraction method like plate and hammer and activating of firecrackers on the surface. The energy released by various sources and frequency spectra was compared for the two types of sources. The obtained results did not determine which sources gave the better results but showed very interesting aspects of using pyrotechnics in seismic measurements for example the use of pyrotechnic materials in MASW.

  12. Plasmodium falciparum: analysis of chromosomes separated by contour-clamped homogenous electric fields.

    Science.gov (United States)

    Gu, H; Inselburg, J W; Bzik, D J; Li, W B

    1990-08-01

    We have established improved conditions for separating the chromosomes of Plasmodium falciparum by pulsed field gradient gel electrophoresis (PFG) using a contour-clamped homogenous electric field (CHEF) apparatus. Thirteen clearly separable chromosomal bands were reproducibly isolated from the strain FCR3 and their sizes have been determined. Evidence that indicates one band may contain two chromosomes is presented. The relationship between the PFG separable DNA and the number of unique chromosomes in P. falciparum is considered. We have established a relationship between the maximum resolvable sizes of the chromosomes and the pulse times. The chromosomal location of twenty-seven P. falciparum DNA probes is also reported. PMID:2197113

  13. Enhanced Buck-Boost Three-Level Neutral-Point-Clamped Inverters

    DEFF Research Database (Denmark)

    Tan, K. K.; Gao, F.; Chiang Loh, Poh; Blaabjerg, Frede

    In traditional three-level neutral-point-clamped (NPC) inverters, a major issue is capacitor voltage imbalance, which results in low order harmonics. The compensation of the capacitor voltages often require additional control complexity, which cannot be conveniently implemented. The "alternative...... method, with the ability to easily adjust for capacitor voltage imbalances. To further improve the boost capability of the three-level NPC inverters, another new topology introduces 2 additional diodes,achieving higher boost performance while totally eliminating thepossibility of capacitor voltage...

  14. Trans-Z-source and Γ-Z-source neutral-point-clamped inverters

    DEFF Research Database (Denmark)

    Wei, Mo; Loh, Poh Chiang; Blaabjerg, Frede; Wang, Peng

    2015-01-01

    Z-source neutral-point-clamped (NPC) inverters are earlier proposed for obtaining voltage buck-boost and three-level switching simultaneously. Their performances are, however, constrained by a trade-off between their input-to-output gain and modulation ratio. This trade-off can lead to high-voltage...... stress across the inverter bridge and poor output voltage spectrum. As alternatives with improved performances, two transformer-based Z-source NPC inverters are proposed, whose gain can be adjusted by varying the transformer turns ratio, and is hence less dependent on the inverter modulation ratio...

  15. HERG channel (dys)function revealed by dynamic action potential clamp technique

    OpenAIRE

    Berecki, G; Zegers, J.G.; Verkerk, A.O.; Bhuiyan, Z.A.; Jonge, de, M.J.I.; Veldkamp, M.W.; Wilders, R; Ginneken, van, CJJM Kees

    2005-01-01

    The human ether-a-go-go-related gene (HERG) encodes the rapid component of the cardiac delayed rectifier potassium current (IKr). Per-Arnt-Sim domain mutations of the HERG channel are linked to type 2 long-QT syndrome. We studied wild-type and/or type 2 long-QT syndrome-associated mutant (R56Q) HERG current (IHERG) in HEK-293 cells, at both 23 and 36°C. Conventional voltage-clamp analysis revealed mutation-induced changes in channel kinetics. To assess functional implication(s) of the mutatio...

  16. Strong localization induced by one clamped point in thin plate vibrations.

    Science.gov (United States)

    Filoche, Marcel; Mayboroda, Svitlana

    2009-12-18

    We discover a strong localization of flexural (bi-Laplacian) waves in rigid thin plates. We show that clamping just one point inside such a plate not only perturbs its spectral properties, but essentially divides the plate into two independently vibrating regions. This effect progressively appears when increasing the plate eccentricity. Such a localization is qualitatively and quantitatively different from the results known for the Laplacian waves in domains of irregular boundary. It would allow us to control the confinement of mechanical vibrations in rigid plates and of eddies in the slow Stokes flow. PMID:20366256

  17. Sodium influxes in internally perfused squid giant axon during voltage clamp.

    Science.gov (United States)

    Atwater, I; Bezanilla, F; Rojas, E

    1969-05-01

    1. An experimental method for measuring ionic influxes during voltage clamp in the giant axon of Dosidicus is described; the technique combines intracellular perfusion with a method for controlling membrane potential.2. Sodium influx determinations were carried out while applying rectangular pulses of membrane depolarization. The ratio ;measured sodium influx/computed ionic flux during the early current' is 0.92 +/- 0.12.3. Plots of measured sodium influx and computed ionic flux during the early current against membrane potential are very similar. There was evidence that the membrane potential at which the sodium influx vanishes is the potential at which the early current reverses. PMID:5767887

  18. Thermal and Efficiency Analysis of Five-Level Multilevel-Clamped Multilevel Converter Considering Grid Codes

    DEFF Research Database (Denmark)

    Ma, Ke; Muñoz-Aguilar, R. S.; Rodriguez, Pedro; Blaabjerg, Frede

    2014-01-01

    In this paper, a five-level multilevel-clamped multilevel converter (5L- MLC2) topology is analyzed based on a typical grid integration application for renewable energy system. The loss and thermal distributions of the power devices in different switching legs are investigated and illustrated under...... rated condition. Afterward, a loss and efficiency evaluation method is proposed and applied which takes into account various injected reactive power ranges regulated by the grid codes. It is concluded that the loss and thermal distributions of the 5L- MLC2 multilevel topology are unequal between the...

  19. Surface analysis of anodized aluminum clamps from NASA-LDEF satellite

    Science.gov (United States)

    Grammer, H. L.; Wightman, J. P.; Young, Philip R.

    1992-01-01

    Surface analysis results of selected anodized aluminum clamps containing black (Z306) and white (A276) paints which received nearly six years of Low Earth Orbit (LEO) exposure on the Long Duration Exposure Facility are reported. Surface analytical techniques, including x-ray photoelectron spectroscopy (XPS), Auger electron spectroscopy (AES), and scanning electron microscopy/energy dispersive analysis by x-ray (SEM/EDAX), showed significant differences in the surface composition of these materials depending upon the position on the LDEF. Differences in the surface composition are attributed to varying amounts of atomic oxygen and vacuum ultraviolet radiation (VUV). Silicon containing compounds were the primary contaminant detected on the materials.

  20. Modeling of Busbars in High Power Neutral Point Clamped Three-Level Inverters

    Institute of Scientific and Technical Information of China (English)

    YI Rung; ZHAO Zhengming; ZHONG Yulin

    2008-01-01

    The busbars in high power neutral point clamped three-level inverters are modeled using the Maxwell Q3D Extractor software, which is based on the partial element equivalent circuits method. The equivalent circuits of the busbars and devices model are simulated in the electric simulator PSIM to analyze the effects of the parasitic inductance on the switching characteristics of the integrated gate commutatedthyristor (IGCT) in different topology positions. The simulation results agree well with the measured imped-ance analyzer results and the IGCT test results, which proves the effectiveness of the modeling method for the large, complex busbars.

  1. Direct-conversion switching-mode audio power amplifier with active capacitive voltage clamp

    Energy Technology Data Exchange (ETDEWEB)

    Ljusev, P.; Andersen, Michael A.E.

    2005-07-01

    This paper discusses the advantages and problems when implementing direct energy conversion switching-mode audio power amplifiers. It is shown that the total integration of the power supply and Class D audio power amplifier into one compact direct converter can simplify design, increase efficiency and integration level, reduce product volume and lower its cost. As an example, the principle of operation and the measurements made on a direct-conversion switching-mode audio power amplifier with active capacitive voltage clamp are presented. (au)

  2. A broadly tunable fiber ring laser employing a gain-clamped semiconductor optical amplifier

    International Nuclear Information System (INIS)

    A widely tunable fiber ring laser is demonstrated experimentally using a specially designed gain-clamped semiconductor optical amplifier (GC-SOA). The 3 dB bandwidth of the generated amplified spontaneous emission is increased by 10 nm using the GC-SOA. The lasing wavelength is continuously tunable in a range from 1522 nm to almost 1600 nm using a thin-film Fabry–Pérot tunable filter. A side-mode-suppression ratio of greater than 55 dB is achieved over the entire tuning range. (paper)

  3. Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish

    OpenAIRE

    Wen, Hua; Brehm, Paul

    2010-01-01

    Larval zebrafish represent the first vertebrate model system to allow simultaneous patch clamp recording from a spinal motor-neuron and target muscle. This is a direct consequence of the accessibility to both cell types and ability to visually distinguish the single segmental CaP motor-neuron on the basis of morphology and location. This video demonstrates the microscopic methods used to identify a CaP motor-neuron and target muscle cells as well as the methodologies for recording from each c...

  4. Effect of La3+ on myocardiac potassium channels revealed by patch-clamp technique

    Institute of Scientific and Technical Information of China (English)

    XUE Shaowu; YANG Pin

    2005-01-01

    The effect of La3+ on potassium channels in rat ventricular myocytes was investigated using the whole-cell patch-clamp recording mode. The Ca2+-independent voltage- activated outward K+ current was activated by the depolarizing pulse in enzymatically isolated rat ventricular myocytes. After addition of different concentrations La3+ to the bath solution, the outward K+ current was depressed gradually. The inhibition effect was in a concentration-dependent manner. The phenomena of the outward K+ current, being the main repolarizing current suppressed by La3+, suggest that the effect of lanthanides on myocardial function should be exploited further.

  5. Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

    OpenAIRE

    Einarsson, Rachel; Haden, Marshall; DiCiolli, Gabrielle; Lim, Andrea; Mah-Ginn, Kolina; Aguilar, Kathleen; Yazejian, Lucy; Yazejian, Bruce

    2012-01-01

    Patch clamp analyses of the voltage-gated channels in sensory hair cells isolated from a variety of species have been described previously1-4 but this video represents the first application of those techniques to hair cells from zebrafish. Here we demonstrate a method to isolate healthy, intact hair cells from all of the inner ear end-organs: saccule, lagena, utricle and semicircular canals. Further, we demonstrate the diversity in hair cell size and morphology and give an example of the kind...

  6. Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials

    OpenAIRE

    Shao, Xuesi M; Feldman, Jack L.

    2006-01-01

    Maintaining a stable electrode potential is critical for patch-clamp measurements. The electrode potential of conventional patch electrode-holder assembly, where an Ag/AgCl wire is in direct contact with the patch pipette filling solution, is subject to drift if the pipette solution contains a low concentration of chloride ions (Cl−). We developed an agar bridge of 3 M KCl filled in a polyimide microtubing which forms an electrical connection between an Ag/AgCl wire and the pipette solution. ...

  7. Voltage-clamp frequency domain analysis of NMDA-activated neurons.

    Science.gov (United States)

    Moore, L E; Hill, R H; Grillner, S

    1993-02-01

    1. Voltage and current-clamp steps were added to a sum of sine waves to measure the tetrodotoxin-insensitive membrane properties of neurons in the intact lamprey spinal cord. A systems analysis in the frequency domain was carried out on two types of cells that have very different morphologies in order to investigate the structural dependence of their electrophysiological properties. The method explicitly takes into account the geometrical shapes of (i) nearly spherical dorsal cells with one or two processes and (ii) motoneurons and interneurons that have branched dendritic structures. Impedance functions were analysed to obtain the cable properties of these in situ neurons. These measurements show that branched neurons are not isopotential and, therefore, a conventional voltage-clamp analysis is not valid. 2. The electrophysiological data from branched neurons were curve-fitted with a lumped soma-equivalent cylinder model consisting of eight equal compartments coupled to an isopotential cell body to obtain membrane parameters for both passive and active properties. The analysis provides a quantitative description of both the passive electrical properties imposed by the geometrical structure of neurons and the voltage-dependent ionic conductances determined by ion channel kinetics. The model fitting of dorsal cells was dominated by a one-compartment resistance and capacitance in parallel (RC) corresponding to the spherical, non-branched shape of these cells. Branched neurons required a model that contained both an RC compartment and a cable that reflected the structure of the cells. At rest, the electrotonic length of the cable was about two. Uniformly distributed voltage-dependent ionic conductance sites were adequate to describe the data at different membrane potentials. 3. The frequency domain admittance method in conjunction with a step voltage clamp was used to control and measure the oscillatory behavior induced by N-methyl-D-aspartate (NMDA) on lamprey spinal

  8. LETTER TO THE EDITOR: Stretching of proteins in a force-clamp

    Science.gov (United States)

    Szymczak, P.; Cieplak, Marek

    2006-01-01

    Mechanically induced protein unfolding in the force-clamp apparatus is shown, in a coarse-grained model of ubiquitin, to have lognormal statistics above a threshold force and exponential statistics below it. Correspondingly, the mean unfolding time is slowly varying and exponentially decreases as a function of the force. The time dependences of the end-to-end distances are also distinct. The time sequence of unfolding events weakly depends on force and much of it resembles that for stretching at constant speed. A more complicated time dependence arises for integrin.

  9. Acute hypotension induced by aortic clamp vs. PTH provokes distinct proximal tubule Na+ transporter redistribution patterns

    DEFF Research Database (Denmark)

    Leong, Patrick K K; Yang, Li E; Lin, Harrison W;

    2004-01-01

    renal cortical membranes fractionated on sorbitol density gradients. Aortic clamp-induced acute hypotension (from 100 +/- 3 to 78 +/- 2 mmHg) provoked a 62% decrease in urine output and a significant decrease in volume flow from the proximal tubule detected as a 66% decrease in endogenous lithium...... clearance. There was, however, no significant change in glomerular filtration rate (GFR) or subcellular distribution of NHE3 and NaPi2. In contrast, high-dose PTH rapidly (<2 min) decreased arterial blood pressure to 51 +/- 3 mmHg, decreased urine output, and shifted NHE3 and NaPi2 out of the low...

  10. Characterization of the insulin sensitivity of ghrelin receptor KO mice using glycemic clamps

    Directory of Open Access Journals (Sweden)

    Morgan Kristen

    2011-01-01

    Full Text Available Abstract Background We and others have demonstrated previously that ghrelin receptor (GhrR knock out (KO mice fed a high fat diet (HFD have increased insulin sensitivity and metabolic flexibility relative to WT littermates. A striking feature of the HFD-fed GhrR KO mouse is the dramatic decrease in hepatic steatosis. To characterize further the underlying mechanisms of glucose homeostasis in GhrR KO mice, we conducted both hyperglycemic (HG and hyperinsulinemic-euglycemic (HI-E clamps. Additionally, we investigated tissue glucose uptake and specifically examined liver insulin sensitivity. Results Consistent with glucose tolerance-test data, in HG clamp experiments, GhrR KO mice showed a reduction in glucose-stimulated insulin release relative to WT littermates. Nevertheless, a robust 1st phase insulin secretion was still achieved, indicating that a healthy β-cell response is maintained. Additionally, GhrR KO mice demonstrated both a significantly increased glucose infusion rate and significantly reduced insulin requirement for maintenance of the HG clamp, consistent with their relative insulin sensitivity. In HI-E clamps, both LFD-fed and HFD-fed GhrR KO mice showed higher peripheral insulin sensitivity relative to WT littermates as indicated by a significant increase in insulin-stimulated glucose disposal (Rd, and decreased hepatic glucose production (HGP. HFD-fed GhrR KO mice showed a marked increase in peripheral tissue glucose uptake in a variety of tissues, including skeletal muscle, brown adipose tissue and white adipose tissue. GhrR KO mice fed a HFD also showed a modest, but significant decrease in conversion of pyruvate to glucose, as would be anticipated if these mice displayed increased liver insulin sensitivity. Additionally, the levels of UCP2 and UCP1 were reduced in the liver and BAT, respectively, in GhrR KO mice relative to WT mice. Conclusions These results indicate that improved glucose homeostasis of GhrR KO mice is

  11. Seismic analysis - what goal

    International Nuclear Information System (INIS)

    The seismic analysis of nuclear components is characterized today by extensive engineering computer calculations in order to satisfy both the component standard codes such as ASME III as well as federal regulations and guides. The current nuclear siesmic design procedure has envolved in a fragmented fashion and continues to change its elements as improved technology leads to changing standards and guides. The dominant trend is a monotonic increase in the overall conservation with time causing a similar trend in costs of nuclear power plants. Ironically the improvements in the state of art are feeding a process which is eroding the very incentives that attracted us to nuclear power in the first place. This paper examines the cause of this process and suggests that what is needed is a realistic goal which appropriately addresses the overall uncertainty of the seismic design process. (Auth.)

  12. Canadian seismic agreement

    International Nuclear Information System (INIS)

    During the period of this report, the contract resources were spent on operation and maintenance of the Eastern Canada Telemetred Network (ECTN), development of special purpose local network systems, servicing and maintenance of the strong-motion seismograph network in eastern Canada, operation of the Ottawa data lab and earthquake monitoring and reporting. Of special note in this period was the final completion of the Sudbury (SLTN) and Charlevoix (CLTN) local networks and the integration of their data processing and analysis requirements in the regular analysis stream for ECTN data. These networks now acquire high quality digital data for detailed analysis of seismic activity and source properties from these two areas, thus effectively doubling the amount of seismic data being received by the Ottawa data lab. 37 refs., 17 figs., 2 tabs

  13. Seismic detection of tornadoes

    Science.gov (United States)

    Tatom, F. B.

    1993-01-01

    Tornadoes represent the most violent of all forms of atmospheric storms, each year resulting in hundreds of millions of dollars in property damage and approximately one hundred fatalities. In recent years, considerable success has been achieved in detecting tornadic storms by means of Doppler radar. However, radar systems cannot determine when a tornado is actually in contact with the ground, expect possibly at extremely close range. At the present time, human observation is the only truly reliable way of knowing that a tornado is actually on the ground. However, considerable evidence exists indicating that a tornado in contact with the ground produces a significant seismic signal. If such signals are generated, the seismic detection and warning of an imminent tornado can become a distinct possibility. 

  14. Seismic Safety Guide

    International Nuclear Information System (INIS)

    The Seismic Safety Guide provides facilities managers with practical guidelines for administering a comprehensive earthquake safety program. Most facilities managers, unfamiliar with earthquake engineering, tend to look for answers in techniques more sophisticated than required to solve the actual problems in earthquake safety. Often the approach to solutions to these problems is so academic, legalistic, and financially overwhelming that mitigation of actual seismic hazards simply does not get done in a timely, cost-effective way. The objective of the Guide is to provide practical advice about earthquake safety so that managers and engineers can get the job done without falling into common pitfalls, prolonged diagnosis, and unnecessary costs. It is comprehensive with respect to earthquakes in that it covers the most important aspects of natural hazards, site planning, rehabilitation of existing buildings, design of new facilities, operational safety, emergency planning, non-structural elements, life lines, and risk management. 5 references

  15. Principle and Program of Evaluating Diffuse Seismicity

    Institute of Scientific and Technical Information of China (English)

    Chang Xiangdong

    2001-01-01

    Concept and origin of the term "the diffuse seismicity" are illustrated. Some different viewpoints regarding the diffuse seismicity and the influence characteristics on determining seismic design basis of engineering from the seismicity are analyzed. Principle and program for evaluating diffuse seismicity are studied and discussed base on over contents.

  16. Oklahoma seismic network

    International Nuclear Information System (INIS)

    The US Nuclear Regulatory Commission has established rigorous guidelines that must be adhered to before a permit to construct a nuclear-power plant is granted to an applicant. Local as well as regional seismicity and structural relationships play an integral role in the final design criteria for nuclear power plants. The existing historical record of seismicity is inadequate in a number of areas of the Midcontinent region because of the lack of instrumentation and (or) the sensitivity of the instruments deployed to monitor earthquake events. The Nemaha Uplift/Midcontinent Geophysical Anomaly is one of five principal areas east of the Rocky Mountain front that has a moderately high seismic-risk classification. The Nemaha uplift, which is common to the states of Oklahoma, Kansas, and Nebraska, is approximately 415 miles long and 12-14 miles wide. The Midcontinent Geophysical Anomaly extends southward from Minnesota across Iowa and the southeastern corner of Nebraska and probably terminates in central Kansas. A number of moderate-sized earthquakes--magnitude 5 or greater--have occurred along or west of the Nemaha uplift. The Oklahoma Geological Survey, in cooperation with the geological surveys of Kansas, Nebraska, and Iowa, conducted a 5-year investigation of the seismicity and tectonic relationships of the Nemaha uplift and associated geologic features in the Midcontinent. This investigation was intended to provide data to be used to design nuclear-power plants. However, the information is also being used to design better large-scale structures, such as dams and high-use buildings, and to provide the necessary data to evaluate earthquake-insurance rates in the Midcontinent

  17. Seismic protocol urges sensitivity

    International Nuclear Information System (INIS)

    This paper is a preliminary summary of a protocol manual for conducting seismic surveys in various topographic and geographic situations to minimize environmental impacts. The manual aims at addressing unique issues for six different types of ecological environments in such a way as to enhance contractors' overall awareness of the types of environmental issues they may encounter, along with some common sense methods of dealing with them. Those six environments are marine, coastal, rain forest, arctic, semi-level terrains, and mountainous

  18. Age depth-model for the past 630 ka in Lake Ohrid (Macedonia/Albania) based on cyclostratigraphic analysis of downhole gamma ray data

    OpenAIRE

    H. Baumgarten; T. Wonik; Tanner, D. C.; A. Francke; Wagner, B.; G. Zanchetta; Sulpizio, R.; Giaccio, B.; Nomade, S.

    2015-01-01

    We report the gamma ray fluctuations from downhole logging data obtained in the sediments of Lake Ohrid from 0 to 240 m below lake floor. These variations in gamma ray and potassium values strongly correlate with fluctuations in global δ18O values and can be thus considered a reliable proxy to depict glacial–interglacial cycles, with high clastic input during cold and/or drier periods and high carbonate precipitation during the warm and/or humid periods in L...

  19. Establishing seismic design criteria to achieve an acceptable seismic margin

    International Nuclear Information System (INIS)

    In order to develop a risk based seismic design criteria the following four issues must be addressed: (1) What target annual probability of seismic induced unacceptable performance is acceptable? (2). What minimum seismic margin is acceptable? (3) Given the decisions made under Issues 1 and 2, at what annual frequency of exceedance should the Safe Shutdown Earthquake ground motion be defined? (4) What seismic design criteria should be established to reasonably achieve the seismic margin defined under Issue 2? The first issue is purely a policy decision and is not addressed in this paper. Each of the other three issues are addressed. Issues 2 and 3 are integrally tied together so that a very large number of possible combinations of responses to these two issues can be used to achieve the target goal defined under Issue 1. Section 2 lays out a combined approach to these two issues and presents three potentially attractive combined resolutions of these two issues which reasonably achieves the target goal. The remainder of the paper discusses an approach which can be used to develop seismic design criteria aimed at achieving the desired seismic margin defined in resolution of Issue 2. Suggestions for revising existing seismic design criteria to more consistently achieve the desired seismic margin are presented

  20. Establishing seismic design criteria to achieve an acceptable seismic margin

    Energy Technology Data Exchange (ETDEWEB)

    Kennedy, R.P. [RPK Structural Mechanics Consulting, Inc., Yorba Linda, CA (United States)

    1997-01-01

    In order to develop a risk based seismic design criteria the following four issues must be addressed: (1) What target annual probability of seismic induced unacceptable performance is acceptable? (2). What minimum seismic margin is acceptable? (3) Given the decisions made under Issues 1 and 2, at what annual frequency of exceedance should the Safe Shutdown Earthquake ground motion be defined? (4) What seismic design criteria should be established to reasonably achieve the seismic margin defined under Issue 2? The first issue is purely a policy decision and is not addressed in this paper. Each of the other three issues are addressed. Issues 2 and 3 are integrally tied together so that a very large number of possible combinations of responses to these two issues can be used to achieve the target goal defined under Issue 1. Section 2 lays out a combined approach to these two issues and presents three potentially attractive combined resolutions of these two issues which reasonably achieves the target goal. The remainder of the paper discusses an approach which can be used to develop seismic design criteria aimed at achieving the desired seismic margin defined in resolution of Issue 2. Suggestions for revising existing seismic design criteria to more consistently achieve the desired seismic margin are presented.

  1. Seismic contracts and agreements

    International Nuclear Information System (INIS)

    Some points to consider regarding management of seismic projects within the Canadian petroleum industry were reviewed. Seismic projects involve the integration of many services. This paper focused on user-provider relationships, the project planning process, competitive bid considerations, the types of agreement used for seismic and their implications, and the impact that certain points of control may have on a company: (1) initial estimate versus actual cost, (2) liability, (3) safety and operational performance, and (4) quality of deliverables. The objective is to drive home the point that in today's environment where companies are forming, merging, or collapsing on a weekly basis , chain of command and accountability are issues that can no longer be dealt with casually. Companies must form business relationships with service providers with a full knowledge of benefits and liabilities of the style of relationship they choose. Diligent and proactive management tends to optimize cost, safety and liability issues, all of which have a bearing on the points of control available to the company

  2. Seismic basement in Poland

    Science.gov (United States)

    Grad, Marek; Polkowski, Marcin

    2016-06-01

    The area of contact between Precambrian and Phanerozoic Europe in Poland has complicated structure of sedimentary cover and basement. The thinnest sedimentary cover in the Mazury-Belarus anteclize is only 0.3-1 km thick, increases to 7-8 km along the East European Craton margin, and 9-12 km in the Trans-European Suture Zone (TESZ). The Variscan domain is characterized by a 1- to 2-km-thick sedimentary cover, while the Carpathians are characterized by very thick sediments, up to c. 20 km. The map of the basement depth is created by combining data from geological boreholes with a set of regional seismic refraction profiles. These maps do not provide data about the basement depth in the central part of the TESZ and in the Carpathians. Therefore, the data set is supplemented by 32 models from deep seismic sounding profiles and a map of a high-resistivity (low-conductivity) layer from magnetotelluric soundings, identified as a basement. All of these data provide knowledge about the basement depth and of P-wave seismic velocities of the crystalline and consolidated type of basement for the whole area of Poland. Finally, the differentiation of the basement depth and velocity is discussed with respect to geophysical fields and the tectonic division of the area.

  3. Impact of early versus delayed umbilical cord clamping on post partum blood loss: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Yogiraj Vaijanathrao Chidre,Vijayalakshmi Chirumamilla

    2015-08-01

    Methods: A randomized, controlled trial was performed on 183 consenting women fulfilling eligibility criteria in labour ward of a teaching hospital in India. They were randomly assigned into early cord clamping or delayed cord clamping group; other components of active management of third stage of labour being same for all. The duration of third stage of labour was noted. Quantitative assessment of post partum blood loss was done using BRASSS-V drapes and weighing blood soaked pads. Cord blood haemoglobin and hematocrit at birth and infant haemoglobin and hematocrit at 90 +/-7 days were estimated. Statistical analysis done using students unpaired and lsquo;t' test. Results: No difference was noted in the mean blood loss (203.52 +/- 122.74 ml versus 200.74+/- 104.07 ml in early and delayed cord clamping respectively and the duration of third stage of labour (Mean: 296.59+/- 98.97 seconds versus 281.79+/-104.59 seconds for early and delayed clamping respectively. Infant haemoglobin at 90 +/-7 days was 11.07 +/- 1.27 gm/dl versus 12.70+/- 1.41 gm/dl (p=0.0000 and infant hematocrit at 90 +/-7 days was 34.13+/-3.93 % versus 39.33 +/-4.88 % in early and delayed clamping respectively (p=0.0000. Conclusion: The timing of cord clamping has no impact on the mean blood loss and duration of third stage of labour. Delayed clamping significantly increases mean venous haemoglobin and hematocrit at 90 +/-7 days without increasing NICU admissions. [Int J Reprod Contracept Obstet Gynecol 2015; 4(4.000: 1103-1108

  4. Age-depth model of the past 630 kyr for Lake Ohrid (FYROM/Albania) based on cyclostratigraphic analysis of downhole gamma ray data

    Science.gov (United States)

    Baumgarten, H.; Wonik, T.; Tanner, D. C.; Francke, A.; Wagner, B.; Zanchetta, G.; Sulpizio, R.; Giaccio, B.; Nomade, S.

    2015-12-01

    Gamma ray (GR) fluctuations and potassium (K) values from downhole logging data obtained in the sediments of Lake Ohrid from 0 to 240 m below lake floor (b.l.f). correlate with fluctuations in δ18O values from the global benthic isotope stack LR04 (Lisiecki and Raymo, 2005). GR and K values are considered a reliable proxy to depict glacial-interglacial cycles, with high clastic input during cold and/or drier periods and high carbonate precipitation during warm and/or humid periods at Lake Ohrid. Spectral analysis was applied to investigate the climate signal and evolution over the length of the borehole. Linking downhole logging data with orbital cycles was used to estimate sedimentation rates and the effect of compaction was compensated for. Sedimentation rates increase on average by 14 % after decompaction of the sediment layers and the mean sedimentation rates shift from 45 cm kyr-1 between 0 and 110 m to 30 cm kyr-1 from 110 to 240 m b.l.f. Tuning of minima and maxima of gamma ray and potassium values versus LR04 extrema, in combination with eight independent tephrostratigraphical tie points, allows establishing of a robust age model for the downhole logging data over the past 630 kyr.

  5. Numerical simulation of downhole temperature distribution in producing oil wells%生产油井井下温度场数值模拟分析

    Institute of Scientific and Technical Information of China (English)

    石颖; 宋延杰; 刘洪

    2008-01-01

    An improved numerical simulation method is presented to calculate the downhole temperature distribution for multiple pay zones in producing oil wells. Based on hydrodynamics and heat transfer theory, a 2-D temperature field model in cylindrical coordinates is developed. In the model, we considered general heat conduction as well as the heat convection due to fluid flow from porous formation to the borehole. We also take into account the fluid velocity variation in the wellbore due to multiple pay zones. We present coupled boundary conditions at the interfaces between the wellbore and adjacent formation, the wellbore and pay zone, and the pay zone and adjacent formation. Finally, an alternating direction implicit difference method (ADI) is used to solve the temperature model for the downhole temperature distribution. The comparison of modeled temperature curve with actual temperature log indicates that simulation result is in general quite similar to the actual temperature log. We found that the total production rate, production time, porosity, thickness of pay zones, and geothermal gradient, all have effects on the downhole temperature distribution.

  6. Age depth-model for the past 630 ka in Lake Ohrid (Macedonia/Albania based on cyclostratigraphic analysis of downhole gamma ray data

    Directory of Open Access Journals (Sweden)

    H. Baumgarten

    2015-05-01

    Full Text Available We report the gamma ray fluctuations from downhole logging data obtained in the sediments of Lake Ohrid from 0 to 240 m below lake floor. These variations in gamma ray and potassium values strongly correlate with fluctuations in global δ18O values and can be thus considered a reliable proxy to depict glacial–interglacial cycles, with high clastic input during cold and/or drier periods and high carbonate precipitation during the warm and/or humid periods in Lake Ohrid. Spectral analysis (sliding window was applied to investigate the climate signal and their evolution over the length of the borehole. Linking the downhole logging data with orbital cycles was used to estimate sedimentation rates, which shift from 45 cm ka−1 between 0 to 110 m to 30 cm ka−1 from 110 to 240 m below lake floor. The effect of compaction was compensated for. Sedimentation rates increase on average by 14% after decompaction of the sediment layers. Tuning of minima and maxima in gamma ray and potassium values vs. LR04 minima and maxima, in combination with eight independent tephrostratigraphical tie points, allows the establishment of a robust age model for the downhole logging data over the past 630 ka in Lake Ohrid.

  7. Detection of gas hydrate with downhole logs and assessment of gas hydrate concentrations (saturations) and gas volumes on the Blake Ridge with electrical resistivity log data

    Science.gov (United States)

    Collett, T.S.; Ladd, J.

    2000-01-01

    Let 164 of the Ocean Drilling Program was designed to investigate the occurrence of gas hydrate in the sedimentary section beneath the Blake Ridge on the southeastern continental margin of North America. Site 994, and 997 were drilled on the Blake Ridge to refine our understanding of the in situ characteristics of natural gas hydrate. Because gas hydrate is unstable at surface pressure and temperature conditions, a major emphasis was placed on the downhole logging program to determine the in situ physical properties of the gas hydrate-bearing sediments. Downhole logging tool strings deployed on Leg 164 included the Schlumberger quad-combination tool (NGT, LSS/SDT, DIT, CNT-G, HLDT), the Formation MicroScanner (FMS), and the Geochemical Combination Tool (GST). Electrical resistivity (DIT) and acoustic transit-time (LSS/SDT) downhole logs from Sites 994, 995, and 997 indicate the presence of gas hydrate in the depth interval between 185 and 450 mbsf on the Blake Ridge. Electrical resistivity log calculations suggest that the gas hydrate-bearing sedimentary section on the Blake Ridge may contain between 2 and 11 percent bulk volume (vol%) gas hydrate. We have determined that the log-inferred gas hydrates and underlying free-gas accumulations on the Blake Ridge may contain as much as 57 trillion m3 of gas.

  8. Seismic hazard assessment of Iran

    Directory of Open Access Journals (Sweden)

    M. Ghafory-Ashtiany

    1999-06-01

    Full Text Available The development of the new seismic hazard map of Iran is based on probabilistic seismic hazard computation using the historical earthquakes data, geology, tectonics, fault activity and seismic source models in Iran. These maps have been prepared to indicate the earthquake hazard of Iran in the form of iso-acceleration contour lines, and seismic hazard zoning, by using current probabilistic procedures. They display the probabilistic estimates of Peak Ground Acceleration (PGA for the return periods of 75 and 475 years. The maps have been divided into intervals of 0.25 degrees in both latitudinal and longitudinal directions to calculate the peak ground acceleration values at each grid point and draw the seismic hazard curves. The results presented in this study will provide the basis for the preparation of seismic risk maps, the estimation of earthquake insurance premiums, and the preliminary site evaluation of critical facilities.

  9. Seismic response of flexible cylindrical tanks

    International Nuclear Information System (INIS)

    An experimental study of the seismic behavior of thin shell circular cylindrical liquid storage tanks is described. The investigation was planned to evaluate the adequacy of present methods of tank design, and was conducted using the Earthquake Simulator Facility of the University of California, Berkeley. The model tank considered in this paper was 6 ft high by 12 ft in diameter, and was welded from thin sheet aluminum to simulate a steel tank 36 feet in diameter. During testing the tank had an open top, held 60 inches of water, and was subjected to a time scaled El Centro (1940) earthquake, amplified to a peak acceleration of 0.5 g. Both base free and base fixed conditions were studied. Results of the experiments demonstrate that fluid pressures included both impulsive and convective components, and that the wave sloshing followed basic theory quite closely. But it also was apparent that the tank flexibility influenced the hydrodynamic pressures, as indicated by pressure amplification in the clamped tank, and by a total change of pressure history in the unclamped case. Significant out of round distortions of the tank were developed, of a three lobe form or the free base case and with four lobes in the fixed base case. Uplift of the tank base was closely related to the out-of-round deformation of the unanchored tank, whereas initial eccentricities apparently caused the section distortions in the anchored system. Stresses in the tank wall do not follow the expected pattern of response to overturning moment; instead they seem to be mainly associated with the section distortions. At present there is no analytical procedure for predicting these distortions

  10. Seismic capacity of a reinforced concrete frame structure without seismic detailing and limited ductility seismic design in moderate seismicity

    International Nuclear Information System (INIS)

    A four-story reinforced concrete frame building model is designed for the gravity loads only. Static nonlinear pushover analyses are performed in two orthogonal horizontal directions. The overall capacity curves are converted into ADRS spectra and compared with demand spectra. At several points the deformed shape, moment and shear distribution are calculated. Based on these results limited ductility seismic design concept is proposed as an alternative seismic design approach in moderate seismicity resign

  11. Direct Torque Control for Three-Level Neutral Point Clamped Inverter-Fed Induction Motor Drive

    Directory of Open Access Journals (Sweden)

    M. K. Sahu

    2012-04-01

    Full Text Available Direct torque control (DTC is a control technique in AC drive systems to obtain high performance torque control. The classical DTC drive contains a pair of hysteresis comparators and suffers from variable switching frequency and high torque ripple. These problems can be solved by using space vector depending on the reference torque and flux. In this paper the space vector modulation technique is applied to the three-level Neutral Point Clamped (NPC inverter control in the proposed DTC-based induction motor drive system, resulting to a significant reduce of torque ripple. Three-level neutral point clamped inverters have been widely used in medium voltage applications. This type of inverters have several advantages over standard two-level VSI, such as greater number of levels in the output voltage waveforms, less harmonic distortion in voltage and current waveforms and lower switching frequencies. This paper emphasizes the derivation of switching states using the Space Vector Pulse Width Modulation (SVPWM technique. The control scheme is implemented using Matlab/Simulink. Experimental results using dSPACE validate the steady-state and the dynamic performance of the proposed control strategy.

  12. Testing of Diode-Clamping in an Inductive Pulsed Plasma Thruster Circuit

    Science.gov (United States)

    Toftul, Alexandra; Polzin, Kurt A.; Martin, Adam K.; Hudgins, Jerry L.

    2014-01-01

    Testing of a 5.5 kV silicon (Si) diode and 5.8 kV prototype silicon carbide (SiC) diode in an inductive pulsed plasma thruster (IPPT) circuit was performed to obtain a comparison of the resulting circuit recapture efficiency,eta(sub r), defined as the percentage of the initial charge energy remaining on the capacitor bank after the diode interrupts the current. The diode was placed in a pulsed circuit in series with a silicon controlled rectifier (SCR) switch, and the voltages across different components and current waveforms were collected over a range of capacitor charge voltages. Reverse recovery parameters, including turn-off time and peak reverse recovery current, were measured and capacitor voltage waveforms were used to determine the recapture efficiency for each case. The Si fast recovery diode in the circuit was shown to yield a recapture efficiency of up to 20% for the conditions tested, while the SiC diode further increased recapture efficiency to nearly 30%. The data presented show that fast recovery diodes operate on a timescale that permits them to clamp the discharge quickly after the first half cycle, supporting the idea that diode-clamping in IPPT circuit reduces energy dissipation that occurs after the first half cycle

  13. A Clamped Be Window for the Dump of the HiRadMat Experiment at CERN

    CERN Document Server

    Delonca, M; Grenier, D; Maglioni, C; Sarrio Martinez, A

    2012-01-01

    At CERN, the High Radiation to Materials facility (HiRadMat) is designed to test accelerator components under the impact of high-intensity pulsed beams and will start operation in 2012. In this frame an LHC TED-type dump was installed at the end of the line, working in nitrogen overpressure, and a 254μm-thick beryllium window was placed as barrier between the inside of the dump and the external atmosphere. Because of the special loading conditions, a clamped window design was especially developed, optimized and implemented, the more standard welded window not being suitable for such loads. Considering then the clamping force and the applied differential pressures, the stresses on the window components were carefully evaluated thanks to empirical as well as numerical models, to guarantee the structural integrity of the beryllium foil. This paper reports on choices and optimizations that led to the final design, presenting also comparative results from different solutions and the detailed results for the adopt...

  14. Mechanically clamped PZT ceramics investigated by First-order reversal curves diagram

    Directory of Open Access Journals (Sweden)

    Laurentiu Stoleriu

    2010-09-01

    Full Text Available The First Order Reversal Curves (FORC diagrams method was developed for characterizing the switching properties of ferroelectrics. In the present paper, the FORC method was applied for hard Pb(Zr,TiO3 ceramics with symmetric and asymmetric clamping. An ideal high-oriented single-crystalline ferroelectric with rectangular P(E loop would be characterised by a delta-function FORC distribution, while real ferroelectrics and mostly the polycrystalline ceramics show dispersed FORC distributions. All the investigated ceramics show FORC distributions with non-Gaussian shape, slightly elongated along the coercitive axis, meaning a high dispersion of the energy barriers separating the two bi-stable polarizations ±P. The degree of dispersion is enhanced by clamping. The maximum FORC coercivity is located at ~ (1.9-2 MV/m for all the hard ceramics. The FORC cycling experiment causes the reversal of the initial poling and result in a positive/negative bias on the FORC diagrams. According to the observed features, it results that FORC coercivity is more related to the nature of the material, while the bias field is more sensitive to the electrical and mechanical boundary conditions in which the ferroelectric ceramics evolves while switching.

  15. MODELING, VALIDATION AND OPTIMAL DESIGN OF THE CLAMPING FORCE CONTROL VALVE USED IN CONTINUOUSLY VARIABLE TRANSMISSION

    Institute of Scientific and Technical Information of China (English)

    ZHOU Yunshan; LIU Jin'gang; CAI Yuanchun; ZOU Naiwei

    2008-01-01

    Associated dynamic performance of the clamping force control valve used in continuously variable transmission (CVT) is optimized. Firstly, the structure and working principle of the valve are analyzed, and then a dynamic model is set up by means of mechanism analysis. For the purpose of checking the validity of the modeling method, a prototype workpiece of the valve is manufactured for comparison test, and its simulation result follows the experimental result quite well. An associated performance index is founded considering the response time, overshoot and saving energy, and five structural parameters are selected to adjust for deriving the optimal associated performance index. The optimization problem is solved by the genetic algorithm (GA) with necessary constraints. Finally, the properties of the optimized valve are compared with those of the prototype workpiece, and the results prove that the dynamic performance indexes of the optimized valve are much better than those of the prototype workpiece.Key words: Dynamic modeling Optimal design Genetic algorithm Clamping force control valve Continuously variable transmission (CVT)

  16. Influence of non-steady state during isoglycemic hyperinsulinemic clamp in hypertension. A LIFE substudy

    DEFF Research Database (Denmark)

    Olsen, M H; Andersen, U B; Wachtell, K;

    1999-01-01

    We wanted to investigate whether time to steady state was reached within 2 h of insulin infusion during isoglycemic hyperinsulinemic clamp, comparing the glucose uptake index (M/IG) with Bergman's insulin sensitivity index (Sip). We performed a 2-h oral glucose tolerance test and a 3-h isoglycemic...... hyperinsulinemic clamp in 26 young, healthy subjects and 43 elderly patients with unmedicated essential hypertension and left ventricular hypertrophy. The 3-h Sip correlated strongly with the 2-h M/IG in the patients (r = 0.88, p <0.001) as well as in the healthy subjects (r = 0.96, p <0.001) with relatively...... narrow limits of agreement in the patients. However, during the third hour of insulin infusion, M/IG (10.0 vs 12.21(2) x kg(-1) x min(-1) x mmol(-1), p <0.001) as well as Sip (7.1 vs 9.41(2) x kg(-1) x min(-1) x mmol(-1), p <0.001) increased significantly in the patients, but not in the healthy subjects...

  17. High Performance ZVT with Bus Clamping Modulation Technique for Single Phase Full Bridge Inverters

    Energy Technology Data Exchange (ETDEWEB)

    Xia, Yinglai; Ayyanar, Raja

    2016-03-20

    This paper proposes a topology based on bus clamping modulation and zero-voltage-transition (ZVT) technique to realize zero-voltage-switching (ZVS) for all the main switches of the full bridge inverters, and inherent ZVS and/or ZCS for the auxiliary switches. The advantages of the strategy include significant reduction in the turn-on loss of the ZVT auxiliary switches which typically account for a major part of the total loss in other ZVT circuits, and reduction in the voltage ratings of auxiliary switches. The modulation scheme and the commutation stages are analyzed in detail. Finally, a 1kW, 500 kHz switching frequency inverter of the proposed topology using SiC MOSFETs has been built to validate the theoretical analysis. The ZVT with bus clamping modulation technique of fixed timing and adaptive timing schemes are implemented in DSP TMS320F28335 resulting in full ZVS for the main switches in the full bridge inverter. The proposed scheme can save up to 33 % of the switching loss compared with no ZVT case.

  18. Indirect Field Oriented Control for Five Phase Three Level Neutral Point Clamped Inverter Fed PMSM Drive

    Directory of Open Access Journals (Sweden)

    S. Sengottaian

    2015-08-01

    Full Text Available This study deals with a five phase three level Neutral Point Clamped (NPC inverter fed PMSM drive application. The motor performances depend upon mathematical model so the parameters vary are: noise, common mode voltage, flux variation and harmonic levels of the inverter or motor. Voltage saturation is one of the major problems of a motor which occurs due to speed oscillations, more current fluctuations. This problem can be solved by using PWM technique depends on the reference motor torque and flux. In this study Indirect Field Oriented Control (IFOC NPC inverter is suggested to reduce the voltage saturation. The three level neutral point clamped inverter is widely used for medium and high level applications. Compared with standard two level inverter, this type of NPC inverters have more merits. It generates greater number of levels output waveform in lower harmonic content at the same switching frequency and less voltage stress across the semiconductor switches; finally motor performance and control schemes are verified by using MATLAB/SIMULINK.

  19. Estimating residual stress, curvature and boundary compliance of doubly clamped MEMS from their vibration response

    International Nuclear Information System (INIS)

    Structural parameters of doubly clamped microfabricated beams such as initial curvature, boundary compliance, thickness and mean residual stress are often critical to the performance of microelectromechanical systems (MEMS) and need to be estimated as a part of quality control of the microfabrication process. However, these parameters couple and influence many metrics of device response and thus are very difficult to disentangle and estimate using conventional methods such as the M-test, static mechanical tests, pull-in measurements or dynamic mechanical tests. Here we present a simple, non-destructive experimental method to extract these parameters based on the non-contact measurement of the natural frequencies of the lowest few eigenmodes of the microfabricated beam, and knowledge of Young's modulus and plan dimensions of the beam alone. The method exploits the fact that certain eigenmodes are insensitive to some of these structural parameters which enable a convenient decoupling and estimation of the parameters. As a result, the method does not require complicated finite element analysis, is insensitive to the gap height and introduces no contact wear or dielectric charging effects. Experiments are performed using laser Doppler vibrometry to measure the natural frequencies of doubly clamped, nickel, RF-MEMS capacitive switches and the method is applied to extract the residual stress, beam thickness, boundary compliance and post-release curvature. (paper)

  20. Temporary clamping of bilateral external carotid arteries for surgical excision of a complex dural arteriovenous fistula

    Directory of Open Access Journals (Sweden)

    Shih-Chieh Shen

    2014-01-01

    Full Text Available Background: Some complex dural arteriovenous fistulas (DAVFs are lesions that typically have numerous arterial feeders. Surgery, including resection of fistulas or skeletonization of the diseased sinus, is still one of the important treatments for these lesions. However, major blood loss is usually encountered during craniotomy because of abundant arterial feeders from the scalp and transosseous vessels. We present a novel approach for obliteration of the fistulas with less blood loss. Methods: Our first case was a 52-year-old male who suffered from syncope and seizure. Cerebral digital subtraction angiography (DSA revealed complex DAVFs with numerous arterial feeders from bilateral external carotid arteries (ECAs and drainage into the superior sagittal sinus with cerebral venous reflux. The second case was a 48-year-old male presenting with chronic headache. His DSA also showed complex DAVFs along the superior sagittal sinus with cerebral venous reflux. In both cases, we performed the surgical procedure to obliterate the pathological fistulas after temporary clamping of bilateral ECAs and noted less blood loss than in the conventional surgery. Results: The follow-up DSA showed successful obliteration of the complex DAVFs on the first case and partial improvement on the second case followed by transarterial embolization (TAE. The symptoms of the both patients were relieved after surgery with good recovery. Conclusion: Temporary clamping of bilateral ECAs can improve the safety and ease the surgical excision for complex DAVFs. By using this technique, neurosurgeons can deal with aggressive DAVFs more confidently and calmly.

  1. A Clamped Be Window for the Dump of the HiRadMat Experiment at CERN

    CERN Document Server

    Delonca, M; Grenier, D; Maglioni, C; Sarrio Martinez, A

    2012-01-01

    At CERN, the High Radiation to Materials facility (HiRadMat) is designed to test accelerator components under the impact of high-intensity pulsed beams and will start operation in 2012. In this frame an LHC TED-type dump was installed at the end of the line, working in nitrogen over-pressure, and a 254μm-thick beryllium window was placed as barrier between the inside of the dump and the external atmosphere. Because of the special loading conditions, a clamped window design was especially developed, optimized and implemented, the more standard welded window not being suitable for such loads. Considering then the clamping force and the applied differential pressures, the stresses on the window components were carefully evaluated thanks to empirical as well as numerical models, to guarantee the structural integrity of the beryllium foil. This paper reports on choices and optimizations that led to the final design, presenting also comparative results from different solutions and the detailed results for the adop...

  2. Interleukin-2 activity can be fine tuned with engineered receptor signaling clamps.

    Science.gov (United States)

    Mitra, Suman; Ring, Aaron M; Amarnath, Shoba; Spangler, Jamie B; Li, Peng; Ju, Wei; Fischer, Suzanne; Oh, Jangsuk; Spolski, Rosanne; Weiskopf, Kipp; Kohrt, Holbrook; Foley, Jason E; Rajagopalan, Sumati; Long, Eric O; Fowler, Daniel H; Waldmann, Thomas A; Garcia, K Christopher; Leonard, Warren J

    2015-05-19

    Interleukin-2 (IL-2) regulates lymphocyte function by signaling through heterodimerization of the IL-2Rβ and γc receptor subunits. IL-2 is of considerable therapeutic interest, but harnessing its actions in a controllable manner remains a challenge. Previously, we have engineered an IL-2 "superkine" with enhanced affinity for IL-2Rβ. Here, we describe next-generation IL-2 variants that function as "receptor signaling clamps." They retained high affinity for IL-2Rβ, inhibiting binding of endogenous IL-2, but their interaction with γc was weakened, attenuating IL-2Rβ-γc heterodimerization. These IL-2 analogs acted as partial agonists and differentially affected lymphocytes poised at distinct activation thresholds. Moreover, one variant, H9-RETR, antagonized IL-2 and IL-15 better than blocking antibodies against IL-2Rα or IL-2Rβ. Furthermore, this mutein prolonged survival in a model of graft-versus-host disease and blocked spontaneous proliferation of smoldering adult T cell leukemia (ATL) T cells. This receptor-clamping approach might be a general mechanism-based strategy for engineering cytokine partial agonists for therapeutic immunomodulation. PMID:25992859

  3. A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode

    KAUST Repository

    Jergic, Slobodan

    2013-02-22

    Processive DNA synthesis by the αÉ"θ core of the Escherichia coli Pol III replicase requires it to be bound to the β 2 clamp via a site in the α polymerase subunit. How the É" proofreading exonuclease subunit influences DNA synthesis by α was not previously understood. In this work, bulk assays of DNA replication were used to uncover a non-proofreading activity of É". Combination of mutagenesis with biophysical studies and single-molecule leading-strand replication assays traced this activity to a novel β-binding site in É" that, in conjunction with the site in α, maintains a closed state of the αÉ"θ-β 2 replicase in the polymerization mode of DNA synthesis. The É"-β interaction, selected during evolution to be weak and thus suited for transient disruption to enable access of alternate polymerases and other clamp binding proteins, therefore makes an important contribution to the network of protein-protein interactions that finely tune stability of the replicase on the DNA template in its various conformational states. © 2013 European Molecular Biology Organization.

  4. Influence of non-steady state during isoglycemic hyperinsulinemic clamp in hypertension. A LIFE substudy

    DEFF Research Database (Denmark)

    Olsen, M H; Andersen, U B; Wachtell, K;

    1999-01-01

    We wanted to investigate whether time to steady state was reached within 2 h of insulin infusion during isoglycemic hyperinsulinemic clamp, comparing the glucose uptake index (M/IG) with Bergman's insulin sensitivity index (Sip). We performed a 2-h oral glucose tolerance test and a 3-h isoglycemic...... narrow limits of agreement in the patients. However, during the third hour of insulin infusion, M/IG (10.0 vs 12.21(2) x kg(-1) x min(-1) x mmol(-1), p <0.001) as well as Sip (7.1 vs 9.41(2) x kg(-1) x min(-1) x mmol(-1), p <0.001) increased significantly in the patients, but not in the healthy subjects....... Because the 2-h M/IG correlated strongly with the 3-h Sip with relatively narrow limits of agreement, it is a good measure of insulin sensitivity. However, a 2-h clamp results in lower insulin sensitivity values in elderly, hypertensive patients due to the fact that steady state is not reached...

  5. Automatic Calibration of Frequency Compensation System in Computer-Controlled Patch-Clamp Amplifier

    Directory of Open Access Journals (Sweden)

    Jun Xion

    2007-01-01

    Full Text Available Computer-controlled patch-clamp amplifier is a digitally controlled analog device used to record the cellular ion channel currents in electrophysiology research. The inherent bandwidth and performance of the headstage is limited by the stray capacitance and distributed capacitance across the feedback resistors. In order to effectively improve the performance of the headstage, the paper advanced a simplified automatic calibration method of frequency compensation system in resistor-feedback patch-clamp amplifier. The dynamic model of headstage was approximate as a two poles and one zero system in the transfer function by experience and test results, so the dynamic characteristics of the headstage were obtained employing least squares parameter estimation algorithm. Further more, the compensation parameter of high frequency booster can be estimated by the time constant of main pole of headstage. And automatic adjustment of the parameters in transient response correction stage was performed as a least squares fitting problem. The software routine running on the host computer conducted all operations of frequency compensation. Experimental results demonstrate that the simplified automatic calibration method can substantially extend the bandwidth and minimize step response error of headstage.

  6. A patch-clamp study on human sperm Cl-channel reassembled into giant liposome

    Institute of Scientific and Technical Information of China (English)

    Jun-Ping BAI; Yu-Liang SHI

    2001-01-01

    Aim: To record the single-channel currents and characterize the electrophysiological properties of the Cl-channels in human sperm membrane. Methods: The membrane proteins extracted from the human sperm were reassembled into liposome bilayer, and the liposomes were fused into giant liposomes with a diameter more than 10 μm by dehydrationrehydration procedure. The giant liposomes were used to study the Cl-channel activities by patch-clamp technique.Results: By patch clamping the giant liposome in an asymmetric NMDG (N-methyl-D-glucamine)-Cl (bath 100//pipette 200 mmol/L) solution system, three kinds of single-channel events with unit conductances of (74.1 ± 8.3) pS,( 117.0 ± 5.7) pS and ( 144.7 ±+ 4.5 ) pS, respectively, were detected. Their activities were voltage-dependent and all manner. By constructing the open and close dwell time distribution histograms and then fitting them with exponential function, two time constants were obtained in both the open and the close states. The burst activity and conductance substate of the channels were observed. Conclusion: There exist three kinds of Cl-channels with different conductance in human sperm membrane at least.

  7. Effect of organometallic clamp properties on the apparent diversity of tensile response of nanowires

    International Nuclear Information System (INIS)

    The influence of the experimental boundary conditions used for tensile testing of individual nanowires on the measured apparent mechanical response is reported. Using a microelectromechanical platform designed for in situ tensile testing, in combination with digital image correlation of sequences of scanning electron microscope images, the mechanical behavior of single crystalline Si, Pd, and Ge2Sb2Te5 nanowires was measured during load–unload cycles. In situ testing enables direct determination of the nanowire strain. Comparison of the direct strain with common metrics for apparent strain that include any compliance or slipping of the clamping materials (electron-beam induced Pt-containing deposits) highlights several different artifacts that may be manifested. Calculation of the contact stiffness is thus enabled, providing guidelines for both proper strain measurement and selection of clamping materials and geometries that facilitate elucidation of intrinsic material response. Our results suggest that the limited ability to tailor the stiffness of electron-beam induced deposits results from the predominance of the organic matrix in controlling its mechanical properties owing to relatively low Pt content and sparse morphology. (paper)

  8. Quantification of material slippage in the iliotibial tract when applying the partial plastination clamping technique.

    Science.gov (United States)

    Sichting, Freddy; Steinke, Hanno; Wagner, Martin F-X; Fritsch, Sebastian; Hädrich, Carsten; Hammer, Niels

    2015-09-01

    The objective of this study was to evaluate the potential of the partial plastination technique in minimizing material slippage and to discuss the effects on the tensile properties of thin dense connective tissue. The ends of twelve iliotibial tract samples were primed with polyurethane resin and covered by plastic plates to provide sufficient grip between the clamps. The central part of the samples remained in an anatomically unfixed condition. Strain data of twelve partially plastinated samples and ten samples in a completely anatomically unfixed state were obtained using uniaxial crosshead displacement and an optical image tracking technique. Testing of agreement between the strain data revealed ongoing but markedly reduced material slippage in partially plastinated samples compared to the unfixed samples. The mean measurement error introduced by material slippage was up to 18.0% in partially plastinated samples. These findings might complement existing data on measurement errors during material testing and highlight the importance of individual quantitative evaluation of errors that come along with self-made clamping techniques. PMID:26005842

  9. Laser microsurgery of higher plant cell walls permits patch-clamp access

    Science.gov (United States)

    Henriksen, G. H.; Taylor, A. R.; Brownlee, C.; Assmann, S. M.; Evans, M. L. (Principal Investigator)

    1996-01-01

    Plasma membranes of guard cells in epidermal peels of Vicia faba and Commelina communis can be made accessible to a patch-clamp pipet by removing a small portion (1-3 micrometers in diameter) of the guard cell wall using a microbeam of ultraviolet light generated by a nitrogen laser. Using this laser microsurgical technique, we have measured channel activity across plasma membranes of V. faba guard cells in both cell-attached and isolated patch configurations. Measurements made in the inside-out patch configuration revealed two distinct K(+)-selective channels. Major advantages of the laser microsurgical technique include the avoidance of enzymatic protoplast isolation, the ability to study cell types that have been difficult to isolate as protoplasts or for which enzymatic isolation protocols result in protoplasts not amenable to patch-clamp studies, the maintenance of positional information in single-channel measurements, reduced disruption of cell-wall-mediated signaling pathways, and the ability to investigate intercellular signaling through studies of cells remaining situated within tissue.

  10. Enhanced brain release of erythropoietin, cytokines and NO during carotid clamping.

    Science.gov (United States)

    Carelli, Stephana; Ghilardi, Giorgio; Bianciardi, Paola; Latorre, Elisa; Rubino, Federico; Bissi, Marina; Di Giulio, Anna Maria; Samaja, Michele; Gorio, Alfredo

    2016-02-01

    Although effective and safe, carotid endarterectomy (CEA) implies a reduced blood flow to the brain and likely an ischemia/reperfusion event. The high rate of uneventful outcomes associated with CEA suggests the activation of brain endogenous protection mechanisms aimed at limiting the possible ischemia/reperfusion damage. This study aims at assessing whether CEA triggers protective mechanisms such as brain release of erythropoietin and nitric oxide. CEA was performed in 12 patients; blood samples were withdrawn simultaneously from the surgically exposed ipsilateral jugular and leg veins before, during (2 and 40 min) and after clamp removal (2 min). Plasma antioxidant capacity, carbonylated proteins, erythropoietin, nitrates and nitrites (NOx) were determined. No changes in intraoperative EEG, peripheral and transcranial blood oxygen saturation were detectable, and no patients showed any neurologic sign after the intervention. Antioxidant capacity and protein carbonylation in plasma were unaffected. Differently, erythropoietin, VEGF, TNF-α and NOx increased during clamping in the jugular blood (2 and 40 min), while no changes were observed in the peripheral circulation. These results show that blood erythropoietin, VEGF, TNF-α, and NOx increased in the brain during uncomplicated CEA. This may represent an endogenous self-activated neuroprotective mechanism aimed at the prevention of ischemia/reperfusion damage. PMID:26494654

  11. Flat lens for seismic waves

    CERN Document Server

    Brule, Stephane; Guenneau, Sebastien

    2016-01-01

    A prerequisite for achieving seismic invisibility is to demonstrate the ability of civil engineers to control seismic waves with artificially structured soils. We carry out large-scale field tests with a structured soil made of a grid consisting of cylindrical and vertical holes in the ground and a low frequency artificial source (< 10 Hz). This allows the identification of a distribution of energy inside the grid, which can be interpreted as the consequence of an effective negative refraction index. Such a flat lens reminiscent of what Veselago and Pendry envisioned for light opens avenues in seismic metamaterials to counteract the most devastating components of seismic signals.

  12. Core seismic methods verification report

    International Nuclear Information System (INIS)

    Information on HTGR reactor core seismic requirements is presented concerning element properties and code parameters; correlation and verification of the codes; sensitivity studies; and application to design

  13. Seismic safety research program plan

    International Nuclear Information System (INIS)

    This document presents a plan for seismic research to be performed by the Structural and Seismic Engineering Branch in the Office of Nuclear Regulatory Research. The plan describes the regulatory needs and related research necessary to address the following issues: uncertainties in seismic hazard, earthquakes larger than the design basis, seismic vulnerabilities, shifts in building frequency, piping design, and the adequacy of current criteria and methods. In addition to presenting current and proposed research within the NRC, the plan discusses research sponsored by other domestic and foreign sources

  14. Attachment ability of a clamp-bearing fish parasite, Diplozoon paradoxum (Monogenea), on gills of the common bream, Abramis brama.

    Science.gov (United States)

    Wong, Wey-Lim; Gorb, Stanislav N

    2013-08-15

    Monogeneans, which are mainly fish ectoparasites, use various types of haptoral (posterior) attachment apparatus to secure their attachment onto their hosts. However, it remains unclear how strongly a monogenean can attach onto its host. In the present study, we aimed for the first time to (1) measure pull-off forces required to detach a pair of clamp-bearing monogeneans, Diplozoon paradoxum, from gills of Abramis brama and (2) determine the contribution of muscles to the clamp movements. A mean force of 6.1±2.7 mN (~246 times the animals' weight) was required to dislodge a paired D. paradoxum vertically from the gills. There were significant differences (Pfiltered lake water (20.16±8.63 μm). This suggests that the closing of the clamps is probably not due to the continuous contraction of extrinsic muscles but is caused by the elasticity of the clamp material and that muscle activity is required for clamp opening. PMID:23580722

  15. An accurate online calibration system based on combined clamp-shape coil for high voltage electronic current transformers

    International Nuclear Information System (INIS)

    Electronic transformers are widely used in power systems because of their wide bandwidth and good transient performance. However, as an emerging technology, the failure rate of electronic transformers is higher than that of traditional transformers. As a result, the calibration period needs to be shortened. Traditional calibration methods require the power of transmission line be cut off, which results in complicated operation and power off loss. This paper proposes an online calibration system which can calibrate electronic current transformers without power off. In this work, the high accuracy standard current transformer and online operation method are the key techniques. Based on the clamp-shape iron-core coil and clamp-shape air-core coil, a combined clamp-shape coil is designed as the standard current transformer. By analyzing the output characteristics of the two coils, the combined clamp-shape coil can achieve verification of the accuracy. So the accuracy of the online calibration system can be guaranteed. Moreover, by employing the earth potential working method and using two insulating rods to connect the combined clamp-shape coil to the high voltage bus, the operation becomes simple and safe. Tests in China National Center for High Voltage Measurement and field experiments show that the proposed system has a high accuracy of up to 0.05 class

  16. del Nido versus St. Thomas Cardioplegia Solutions: A Single-Center Retrospective Analysis of Post Cross-Clamp Defibrillation Rates.

    Science.gov (United States)

    Buel, Shane T; Striker, Carrie Whittaker; O'Brien, James E

    2016-06-01

    There are many cardioplegia solutions currently in use for pediatric cardiopulmonary bypass (CPB). The most common being del Nido solution. Another common cardioplegia solution used for pediatric CPB is St. Thomas. In October 2014, Children's Mercy Kansas City changed from the use of modified St. Thomas to del Nido. This study compared rates of post cross-clamp fibrillation requiring defibrillation between del Nido solution and modified St. Thomas solution stratified by weight at Children's Mercy Kansas City. This retrospective study consisted of 394 patients who underwent cardiac surgery requiring cardioplegia between January 1, 2014 and July 31, 2015. The outcome measured was defibrillation upon cross-clamp removal. Statistical significance was determined using Fishers exact test with a two-sided significance level of .05. Incidence of defibrillation post cross-clamp removal was 4.4% in the del Nido group and 26.8% in the St. Thomas group (p 60-kg category had an incidence of defibrillation of 16.7% in the del Nido group and 63% in the St. Thomas group (p < .0623). This study demonstrates a 6-fold decrease in the overall rate of defibrillation post cross-clamp removal between St. Thomas and del Nido cardioplegia solutions. Analyses of weight stratifications demonstrate a decrease in the rate of defibrillation post cross-clamp removal in all categories within the del Nido group. PMID:27578896

  17. Influence of frozen ground on seismic data

    OpenAIRE

    Eide, Cathrine Tangerås

    2012-01-01

    The interest for subsurface exploration in the arctic is increasing. Thus knowledge about the effects of glaciers and permafrost on seismic data is needed. This thesis focuses on five questions that are relevant in this respect: How do the seismic velocities of the glacier influence seismic data? How does the glacier thickness affect the seismic data? How does the permafrost affect the seismic data? How do thickness variations of the permafrost layer affect the seismic data? How do saturation...

  18. Downhole geophysical data from recent deep drilling in the center of the Thuringian Basin, Germany

    Science.gov (United States)

    Methe, Pascal; Goepel, Andreas; Kukowski, Nina

    2014-05-01

    In the framework of the INFLUINS (Integrated Fluid Dynamics in Sedimentary Basins) project, a 1.179 meter deep scientific borehole was drilled in summer 2013. The drill site is situated in the north of Erfurt, in the center of the Thuringian Basin on the crossing point of two seismic reflection profiles, which were acquired in 2011. An almost complete sequence from Keuper to the base of the Buntsandstein was drilled. Drilling, geophysical measurements and well construction were conducted for three depth intervals. First, drilling was undertaken to a depth of 313 m down to the top of the Middle Muschelkalk. Then, the Middle and Upper Muschelkalk were drilled to a depth of 515 m and the third part of the drilling campaign was finished at a depth of 1.179 m at the base of the Lower Buntsandstein. Coring was done in the depth intervals of 285 m to 420 m and 520 m to 914 m. With the help of the borehole geophysical measurements, which were done along the entire depth, stratigraphic information obtained through core samples can be extrapolated from cored sections into those depth sections, where no coring was done. Immediately after finishing drilling through a certain depth interval, borehole geophysical measurements were conducted in the open hole. Using the caliper and inclination instruments, the geometry of the well was determined. In addition, milieu, gamma-ray, spectral gamma-ray, acoustic borehole television, sonic, susceptibility, dipmeter, gamma-gamma, neutron-neutron and the dual latero-log were measured to get information about rock properties. Within rock-salt bearing depth intervals, embedded cm-thin layers of clay can be geophysically resolved. This will e.g. enable to determine and contrast the physical properties of these alternating sequences with high accuracy. Besides the in-situ well measurements rock-physical parameters of the core samples were acquired with a Multi-Sensor Core Logger (MSCL). Here, we present the new data set and discuss some

  19. Seismic monitoring of geomorphic processes

    Science.gov (United States)

    Burtin, A.; Hovius, N.; Turowski, J. M.

    2014-12-01

    In seismology, the signal is usually analysed for earthquake data, but these represent less than 1% of continuous recording. The remaining data are considered as seismic noise and were for a long time ignored. Over the past decades, the analysis of seismic noise has constantly increased in popularity, and this has led to develop new approaches and applications in geophysics. The study of continuous seismic records is now open to other disciplines, like geomorphology. The motion of mass at the Earth's surface generates seismic waves that are recorded by nearby seismometers and can be used to monitor its transfer through the landscape. Surface processes vary in nature, mechanism, magnitude and space and time, and this variability can be observed in the seismic signals. This contribution aims to give an overview of the development and current opportunities for the seismic monitoring of geomorphic processes. We first describe the common principles of seismic signal monitoring and introduce time-frequency analysis for the purpose of identification and differentiation of surface processes. Second, we present techniques to detect, locate and quantify geomorphic events. Third, we review the diverse layout of seismic arrays and highlight their advantages and limitations for specific processes, like slope or channel activity. Finally, we illustrate all these characteristics with the analysis of seismic data acquired in a small debris-flow catchment where geomorphic events show interactions and feedbacks. Further developments must aim to fully understand the richness of the continuous seismic signals, to better quantify the geomorphic activity and improve the performance of warning systems. Seismic monitoring may ultimately allow the continuous survey of erosion and transfer of sediments in the landscape on the scales of external forcing.

  20. Seismic failure modes and seismic safety of Hardfill dam

    Institute of Scientific and Technical Information of China (English)

    Kun XIONG; Yong-hong WENG; Yun-long HE

    2013-01-01

    Based on microscopic damage theory and the finite element method, and using the Weibull distribution to characterize the random distribution of the mechanical properties of materials, the seismic response of a typical Hardfill dam was analyzed through numerical simulation during the earthquakes with intensities of 8 degrees and even greater. The seismic failure modes and failure mechanism of the dam were explored as well. Numerical results show that the Hardfill dam remains at a low stress level and undamaged or slightly damaged during an earthquake with an intensity of 8 degrees. During overload earthquakes, tensile cracks occur at the dam surfaces and extend to inside the dam body, and the upstream dam body experiences more serious damage than the downstream dam body. Therefore, under the seismic conditions, the failure pattern of the Hardfill dam is the tensile fracture of the upstream regions and the dam toe. Compared with traditional gravity dams, Hardfill dams have better seismic performance and greater seismic safety.

  1. Bayesian seismic AVO inversion

    Energy Technology Data Exchange (ETDEWEB)

    Buland, Arild

    2002-07-01

    A new linearized AVO inversion technique is developed in a Bayesian framework. The objective is to obtain posterior distributions for P-wave velocity, S-wave velocity and density. Distributions for other elastic parameters can also be assessed, for example acoustic impedance, shear impedance and P-wave to S-wave velocity ratio. The inversion algorithm is based on the convolutional model and a linearized weak contrast approximation of the Zoeppritz equation. The solution is represented by a Gaussian posterior distribution with explicit expressions for the posterior expectation and covariance, hence exact prediction intervals for the inverted parameters can be computed under the specified model. The explicit analytical form of the posterior distribution provides a computationally fast inversion method. Tests on synthetic data show that all inverted parameters were almost perfectly retrieved when the noise approached zero. With realistic noise levels, acoustic impedance was the best determined parameter, while the inversion provided practically no information about the density. The inversion algorithm has also been tested on a real 3-D dataset from the Sleipner Field. The results show good agreement with well logs but the uncertainty is high. The stochastic model includes uncertainties of both the elastic parameters, the wavelet and the seismic and well log data. The posterior distribution is explored by Markov chain Monte Carlo simulation using the Gibbs sampler algorithm. The inversion algorithm has been tested on a seismic line from the Heidrun Field with two wells located on the line. The uncertainty of the estimated wavelet is low. In the Heidrun examples the effect of including uncertainty of the wavelet and the noise level was marginal with respect to the AVO inversion results. We have developed a 3-D linearized AVO inversion method with spatially coupled model parameters where the objective is to obtain posterior distributions for P-wave velocity, S

  2. Borehole seismic modeling

    Science.gov (United States)

    Zhen, Tao

    In many borehole seismic experiments, the velocity of the tube wave is higher than that of the surrounding rock shear wave. This fast tube wave creates a strong conical shear wave in the surrounding rock, similar to the Mach wave in supersonic aviation and the Cherenkov radiation in electrodynamics. Many geophysicists have tried to utilize the conical signal in VSP (vertical seismic profiling) and cross borehole data interpretation, using quasi static approximations to model the borehole effect. Two popular quasi static approximations are: the effective source array method for source borehole modeling and the squeeze strain method for receiver borehole modeling. These quasi static approximations are sensible as they qualitatively conform to Hueygen's principle and the typical wavelength of a VSP or a cross borehole seismic experiment is much larger than the borehole radius. However, they have not been quantitatively benchmarked against other non approximation method such as the frequency wave number method. The frequency wave number method is a rigorous, non approximation method for modeling straight boreholes without lengthwise variation. The boreholes may consist of many coaxial, homogeneous and axially symmetric shells. In this thesis, the results of the quasi static approximations are compared to the results obtained from the frequency wave number method. The comparison demonstrates that both the effective source array method and squeeze strain method gives the correct arrival time. The effective source array method gives incorrect amplitude and waveform for direct arrivals and tube waves due to its arbitrary assumption of the elementary source radiation pattern. The squeeze strain method gives fairly accurate amplitude and waveform for P and S direct arrivals but it fails to match the tube wave results obtained from the frequency wave number method. The omission of tube wave dispersion and amplitude loss by the quasi static approximation methods also

  3. Seismic Network Deployment Preparations

    OpenAIRE

    Allen Husker; Igor Stubailo; Monica Kohler; Paul Davis

    2003-01-01

    Technological and scientific preparations are occurring for the development of a multi-hop radio-linked seismic array (MHRLSA) of 50 broadband stations (GURALP 3ts) and its first few deployments. A ruggedized data relay device (DRD) is being fabricated using Intel’¡Çs new low power, small form factor stargate motherboard. A DRD will be placed at each node of the array and configured as a local area network (LAN) with station spacing up to 10 km. The objective is to use protocols that have bee...

  4. Seismic risk perception test

    Science.gov (United States)

    Crescimbene, Massimo; La Longa, Federica; Camassi, Romano; Pino, Nicola Alessandro

    2013-04-01

    The perception of risks involves the process of collecting, selecting and interpreting signals about uncertain impacts of events, activities or technologies. In the natural sciences the term risk seems to be clearly defined, it means the probability distribution of adverse effects, but the everyday use of risk has different connotations (Renn, 2008). The two terms, hazards and risks, are often used interchangeably by the public. Knowledge, experience, values, attitudes and feelings all influence the thinking and judgement of people about the seriousness and acceptability of risks. Within the social sciences however the terminology of 'risk perception' has become the conventional standard (Slovic, 1987). The mental models and other psychological mechanisms which people use to judge risks (such as cognitive heuristics and risk images) are internalized through social and cultural learning and constantly moderated (reinforced, modified, amplified or attenuated) by media reports, peer influences and other communication processes (Morgan et al., 2001). Yet, a theory of risk perception that offers an integrative, as well as empirically valid, approach to understanding and explaining risk perception is still missing". To understand the perception of risk is necessary to consider several areas: social, psychological, cultural, and their interactions. Among the various research in an international context on the perception of natural hazards, it seemed promising the approach with the method of semantic differential (Osgood, C.E., Suci, G., & Tannenbaum, P. 1957, The measurement of meaning. Urbana, IL: University of Illinois Press). The test on seismic risk perception has been constructed by the method of the semantic differential. To compare opposite adjectives or terms has been used a Likert's scale to seven point. The test consists of an informative part and six sections respectively dedicated to: hazard; vulnerability (home and workplace); exposed value (with reference to

  5. Iberian seismicity, 1961 - 1965

    Directory of Open Access Journals (Sweden)

    MUNUERA J. M.

    1967-06-01

    Full Text Available This paper compares tlie seismic results corresponding
    to 1961-65 with those presented in several a u t h o r ' s prior studies for the
    I b e r i a n Peninsula region, which were obtained reducing to a 50 y e a r period
    t h e available seismic material up to 1960. The present paper lists 237
    e a r t h q u a k e s , a selection between those occurred and recorded; their epic
    e n t r a l coordinates, origin time, depth and magnitude f r om body waves;
    and a discussion about t h e seismically active quadricles on t h e area, regional
    density, epicenter display, annual frequency in steps of % m> t h e parabolic
    a d j u s t m e n t for log N, m, tectonic flux and i n t e r m e d i a t e foci location rel
    a t e d with the main tectonic features.
    1961-65 is a t e n t h of t h e foresaid reduced 50 Y.P. and the present
    r e s u l t s agree with t h e preliminary ones, as it was expected; mainly for the
    following features: a migration of maximal density which is Easterly;
    b a s a t i s f a c t o r y parabolic regression for log JVT, m; c t h e cyclic appearance
    r e g a r d i n g annual frequency v a r i a t i o n ; d continuation of the accumulative
    behaviour for the strain release curve during the present period, which
    began in 1954; e seismicity is significatively of an i n t r a l p i n e character,
    and / i n t e r m e d i a t e foci (preliminary determinations laying out by line
    with both: Guadalquivir Fault, SW extension, and Alboran Sea's bottoms

  6. Seismic wave generator

    International Nuclear Information System (INIS)

    This invention concerns a device for simulating earth tremors. This device includes a seismic wave generator formed of a cylinder, one end of which is closed by one of the walls of a cell containing a soil, the other end being closed by a wall on which are fixed pyrotechnic devices generating shock waves inside the cylinder. These waves are transmitted from the cylinder to the cell through openings made in the cell wall. This device also includes a mechanical device acting as low-pass filter, located inside the cylinder and close to the cell wall

  7. Intercluster Correlation in Seismicity

    CERN Document Server

    Helmstetter, A; Helmstetter, Agnes; Sornette, Didier

    2003-01-01

    Mega et al.(cond-mat/0212529) proposed to use the "diffusion entropy" (DE) method to demonstrate that the distribution of time intervals between a large earthquake (the mainshock of a given seismic sequence) and the next one does not obey Poisson statistics. We have performed synthetic tests which show that the DE is unable to detect correlations between clusters, thus negating the claimed possibility of detecting an intercluster correlation. We also show that the LR model, proposed by Mega et al. to reproduce inter-cluster correlation, is insufficient to account for the correlation observed in the data.

  8. Seismic surveying and accelerators

    International Nuclear Information System (INIS)

    The paper deals with an investigation into the impact of earth vibrations on charged particle beams in modern colliders. It is ascertained that the displacement of accelerator magnetic elements from the perfect position results in the excitation of betatron oscillations and distortion of particle orbit position. The results of experimental investigations into seismic noises are presented for ASR, SSC, DESY and KEK. The rms orbit displacement in accelerators is estimated relying on the law of earth diffusion motion, according to which the variance of relative displacements is proportional to the distance between these points and time of observation. 6 refs., 3 figs., 2 tabs

  9. A Hybrid Cascade Converter Topology With Series-Connected Symmetrical and Asymmetrical Diode-Clamped H-Bridge Cells

    DEFF Research Database (Denmark)

    Nami, Alireza; Zare, Firuz; Ghosh, Arindam; Blaabjerg, Frede

    2011-01-01

    A novel H-bridge multilevel pulsewidth modulation converter topology based on a series connection of a high-voltage diode-clamped inverter and a low-voltage conventional inverter is proposed in this paper. A dc link voltage arrangement for the new hybrid and asymmetric solution is presented to have...... configuration to approach a very low total harmonic distortion of voltage and current, which leads to the possible elimination of the output filter. Regarding the proposed configuration, a new cascade inverter is verified by cascading an asymmetrical diode-clamped inverter, in which 19 levels can be synthesized...... in output voltage with the same number of components. To balance the dc link capacitor voltages for the maximum output voltage resolution as well as synthesize asymmetrical dc link combination, a new multi-output boost converter is utilized at the dc link voltage of a seven-level H-bridge diode-clamped...

  10. Comparison: Simulation and Experimental Characterisation of an all-Optical Gain-Clamped Erbium-Doped Fibre Amplifier

    Directory of Open Access Journals (Sweden)

    T. Subramaniam M. A. Mahdi P. Poopalan, and H. Ahmad

    2012-10-01

    Full Text Available This paper presents the GC-EDFA characteristics comparison between the simulated results (using EDFA_Design software and experimental results. The comparisons reveal the usefulness of the software in simulating the behaviour of an all-optical GC-EDFA system. Comparisons are made for values obtained from the system operated at high laser power, in order to highlight the differences between the experimental and simulated values. The main objective for this comparison is to prove the capability of the software in simulating the gain-clamped system. Therefore, the software can be used to test new configurations, aimed at improvising current gain-clamped EDFA performances.Keywords optical, gain-clamping, erbium, fibre amplifier, simulation, experiment

  11. Negligible substrate clamping effect on piezoelectric response in (111)-epitaxial tetragonal Pb(Zr, Ti)O{sub 3} films

    Energy Technology Data Exchange (ETDEWEB)

    Yamada, Tomoaki, E-mail: t-yamada@nucl.nagoya-u.ac.jp [Department of Materials, Physics and Energy Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan); PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012 (Japan); Yasumoto, Jun; Ito, Daisuke; Yoshino, Masahito; Nagasaki, Takanori [Department of Materials, Physics and Energy Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan); Sakata, Osami [Synchrotron X-ray Station at SPring-8 and Synchrotron X-ray Group, National Institute for Materials Science, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan); Department of Innovative and Engineered Material, Tokyo Institute of Technology, Yokohama 226-8503 (Japan); Imai, Yasuhiko [Japan Synchrotron Radiation Research Institute, SPring-8, Sayo-cho, Sayo-gun, Hyogo 679-5198 (Japan); Kiguchi, Takanori [Institute for Materials Research, Tohoku University, Sendai, Miyagi 980-8577 (Japan); Shiraishi, Takahisa; Shimizu, Takao; Funakubo, Hiroshi [Department of Innovative and Engineered Material, Tokyo Institute of Technology, Yokohama 226-8503 (Japan)

    2015-08-21

    The converse piezoelectric responses of (111)- and (001)-epitaxial tetragonal Pb(Zr{sub 0.35}Ti{sub 0.65})O{sub 3} [PZT] films were compared to investigate the orientation dependence of the substrate clamping effect. Synchrotron X-ray diffraction (XRD) and piezoelectric force microscopy revealed that the as-grown (111)-PZT film has a polydomain structure with normal twin boundaries that are changed by the poling process to inclined boundaries, as predicted by Romanov et al. [Phys. Status Solidi A 172, 225 (1999)]. Time-resolved synchrotron XRD under bias voltage showed the negligible impact of substrate clamping on the piezoelectric response in the (111)-PZT film, unlike the case for (001)-PZT film. The origin of the negligible clamping effect in the (111)-PZT film is discussed from the viewpoint of the elastic properties and the compensation of lattice distortion between neighboring domains.

  12. Stress-stimulated current of dry rocks with constant clamping stress

    Science.gov (United States)

    Dahlgren, R. P.; Vanderbilt, V. C.; Johnston, M. J. S.

    2014-12-01

    A set of nominally dry rocks (gabbro, granite, limestone, marble, and sandstone) were subjected to asymmetric loading with a large hydraulic press. A pair of precision platens made from 1018 low carbon steel were used to apply uniaxial compressive stress (σ) to the sample, via a thin electrical insulator made from ultra-high molecular weight (UHMW) polyethylene. Self-adhesive copper electrodes were applied and burnished on the end faces and the stress-stimulated current (SSC) was monitored using a Keithley 617 instrument. A preload stress level of 5.5 MPa was applied to firmly clamp the assembly throughout the experiment. From this baseline, σ was increased to 22.25 MPa and held for 100 seconds before returning to the clamping stress level. This loading profile was repeated for four or more cycles, with a stress rate on the order of 5MPa/sec. After the first load cycle, the SSC transients (and SSV offsets) are reversible when σ returned to its baseline level. All samples showed alternating unipolar SSC transients at the beginning and end of each load cycle. SSC from limestone, Westerly granite and marble were at, or below, the measurement limit (±1 pA). All other samples except sandstone showed a negative SSC with increasing stress. For stress-stimulated voltage (SSV) there was a richer variety of transients observed such as unipolar, bipolar and more complex transient dynamics. Limestone was the only sample tested with no SSV transients although this particular rock had a major calcite inclusion in the sample. White granite tended to have the least stable SSC and SSV values. Of the six different rock samples tested under identical conditions, the SSC and SSV observed were not greater than -15 pA, presumably due to improved experimental procedures. The response for rocks with semiconductor properties (gabbro, granite) is the same as those without semiconductor properties (limestone, marble), although the values for marble were below the noise. For repetitive

  13. Seismic Data Gathering and Validation

    Energy Technology Data Exchange (ETDEWEB)

    Coleman, Justin [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-02-01

    Three recent earthquakes in the last seven years have exceeded their design basis earthquake values (so it is implied that damage to SSC’s should have occurred). These seismic events were recorded at North Anna (August 2011, detailed information provided in [Virginia Electric and Power Company Memo]), Fukushima Daichii and Daini (March 2011 [TEPCO 1]), and Kaswazaki-Kariwa (2007, [TEPCO 2]). However, seismic walk downs at some of these plants indicate that very little damage occurred to safety class systems and components due to the seismic motion. This report presents seismic data gathered for two of the three events mentioned above and recommends a path for using that data for two purposes. One purpose is to determine what margins exist in current industry standard seismic soil-structure interaction (SSI) tools. The second purpose is the use the data to validated seismic site response tools and SSI tools. The gathered data represents free field soil and in-structure acceleration time histories data. Gathered data also includes elastic and dynamic soil properties and structural drawings. Gathering data and comparing with existing models has potential to identify areas of uncertainty that should be removed from current seismic analysis and SPRA approaches. Removing uncertainty (to the extent possible) from SPRA’s will allow NPP owners to make decisions on where to reduce risk. Once a realistic understanding of seismic response is established for a nuclear power plant (NPP) then decisions on needed protective measures, such as SI, can be made.

  14. Active and passive seismic methods for characterization and monitoring of unstable rock masses: field surveys, laboratory tests and modeling.

    Science.gov (United States)

    Colombero, Chiara; Baillet, Laurent; Comina, Cesare; Jongmans, Denis; Vinciguerra, Sergio

    2016-04-01

    Appropriate characterization and monitoring of potentially unstable rock masses may provide a better knowledge of the active processes and help to forecast the evolution to failure. Among the available geophysical methods, active seismic surveys are often suitable to infer the internal structure and the fracturing conditions of the unstable body. For monitoring purposes, although remote-sensing techniques and in-situ geotechnical measurements are successfully tested on landslides, they may not be suitable to early forecast sudden rapid rockslides. Passive seismic monitoring can help for this purpose. Detection, classification and localization of microseismic events within the prone-to-fall rock mass can provide information about the incipient failure of internal rock bridges. Acceleration to failure can be detected from an increasing microseismic event rate. The latter can be compared with meteorological data to understand the external factors controlling stability. On the other hand, seismic noise recorded on prone-to-fall rock slopes shows that the temporal variations in spectral content and correlation of ambient vibrations can be related to both reversible and irreversible changes within the rock mass. We present the results of the active and passive seismic data acquired at the potentially unstable granitic cliff of Madonna del Sasso (NW Italy). Down-hole tests, surface refraction and cross-hole tomography were carried out for the characterization of the fracturing state of the site. Field surveys were implemented with laboratory determination of physico-mechanical properties on rock samples and measurements of the ultrasonic pulse velocity. This multi-scale approach led to a lithological interpretation of the seismic velocity field obtained at the site and to a systematic correlation of the measured velocities with physical properties (density and porosity) and macroscopic features of the granitic cliff (fracturing, weathering and anisotropy). Continuous

  15. An Experimental Method for Measuring the Clamping Force in Double Lap Simple Bolted and Hybrid (Bolted-Bonded) Joints

    OpenAIRE

    Goldarag, Firooz Esmaeili; Barzegar, Sajjad; Babaei, Alireza

    2015-01-01

    In this research, an experimental method for measuring the clamping force as a result of tightening torque in double lap simple bolted and hybrid (bolted-bonded) joints is proposed. Two types of joints, i.e. double lap simple and hybrid (bolted-bonded) joints were prepared for testing. In order to measure the clamping force or pretension resulting from the tightening torque at different applied torques, for both types of joints, a special experimental method was designed using a steel bush th...

  16. A novel latch-up free SCR-LDMOS with high holding voltage for a power-rail ESD clamp

    International Nuclear Information System (INIS)

    The low snapback holding voltage of the SCR-LDMOS device makes it susceptible to latch-up failure, when used in power-rail ESD (electro-static discharge) clamp circuits. In order to eliminate latch-up risk, this work presents a novel SCR-LDMOS structure with an N-type implantation layer to achieve a 17 V holding voltage and a 5.2 A second breakdown current. The device has been validated using TLP measurement analysis and is applied to a power-rail ESD clamp in half-bridge driver ICs. (semiconductor devices)

  17. A novel latch-up free SCR-LDMOS with high holding voltage for a power-rail ESD clamp

    Institute of Scientific and Technical Information of China (English)

    Pan Hongwei; Liu Siyang; Sun Weifeng

    2013-01-01

    The low snapback holding voltage of the SCR-LDMOS device makes it susceptible to latch-up failure,when used in power-rail ESD (electro-static discharge) clamp circuits.In order to eliminate latch-up risk,this work presents a novel SCR-LDMOS structure with an N-type implantation layer to achieve a 17 V holding voltage and a 5.2 A second breakdown current.The device has been validated using TLP measurement analysis and is applied to a power-rail ESD clamp in half-bridge driver ICs.

  18. Seismicity of Texas Panhandle

    International Nuclear Information System (INIS)

    Seismicity of the Texas Panhandle has been examined to evaluate the earthquake hazard at a potential site for a high-level nuclear waste repository in the Palo Duro Basin. The Texas Panhandle is an area of low seismicity. The first earthquake report for the area dates back to 1907. Several earthquakes have been felt since then, although none of them have caused any damage. Most of the earthquakes occurred in the northern part of the Texas Panhandle and have been assigned locations of felt reports. A sixteen-station seismographic network was installed in the Texas Panhandle so earthquakes occurring in the area could be accurately located. The network became fully operational in October 1984 and has recorded a few small earthquakes occurring in the northern part of the Panhandle. These earthquakes range in magnitude up to about 3.1, but were not felt. The earthquakes appear to be associated with either the Amarillo Uplift or the Oldham Nose. The limited data do not permit fault plane solutions or associate the earthquakes with specific faults in these two structures. The Palo Duro Basin appears to be nearly aseismic. Although historical reports indicate association of earthquakes with Amarillo Uplift, the occurrence of microearthquakes in the Oldham Nose area was unknown until the microearthquake monitoring network was installed

  19. Civil Works Seismic Designs

    International Nuclear Information System (INIS)

    RFS or Regles Fondamentales de Surete (Basic Safety Rules) applicable to certain types of nuclear facilities lay down requirements with which compliance, for the type of facilities and within the scope of application covered by the RFS, is considered to be equivalent to compliance with technical French regulatory practice. The object of the RFS is to take advantage of standardization in the field of safety, while allowing for technical progress in that field. They are designed to enable the operating utility and contractors to know the rules pertaining to various subjects which are considered to be acceptable by the Service Central de Surete des Installations Nucleaires, or the SCSIN (Central Department for the Safety of Nuclear Facilities). These RFS should make safety analysis easier and lead to better understanding between experts and individuals concerned with the problems of nuclear safety. The SCSIN reserves the right to modify, when considered necessary, any RFS and specify, if need be, the terms under which a modification is deemed retroactive. This rule defines: - the parameters characterizing the design seismic motions - the calculation methods - the mathematical schematization principles on which calculations are based - the use of the seismic response for the structure checking - the content of the documents to be presented

  20. Nonlinear dynamic behaviors of clamped laminated shallow shells with one-to-one internal resonance

    Science.gov (United States)

    Abe, Akira; Kobayashi, Yukinori; Yamada, Gen

    2007-07-01

    This paper investigates one-to-one internal resonance of laminated shallow shells with rigidly clamped edges. It is assumed that the natural frequencies ω2 and ω3 of two asymmetric (second and third) vibration modes have the relationship ω2≈ ω3. The displacements are expressed by using eigenvectors for linear vibration modes calculated by the Ritz method. Applying Galerkin's procedure to the equation of motion, nonlinear differential equations are derived. By considering the first vibration mode in addition to the two asymmetric vibration modes, quadratic nonlinear terms expressing the interaction between the asymmetric and the first modes appear in the differential equations. Shooting method is used to obtain the steady-state response when the driving frequency Ω is near ω2. The dynamic characteristics of the shells with the internal resonance are discussed.

  1. Fabrication and characterization of GaN nanowire doubly clamped resonators

    Science.gov (United States)

    Maliakkal, Carina B.; Mathew, John P.; Hatui, Nirupam; Rahman, A. Azizur; Deshmukh, Mandar M.; Bhattacharya, Arnab

    2015-09-01

    Gallium nitride (GaN) nanowires (NWs) have been intensely researched as building blocks for nanoscale electronic and photonic device applications; however, the mechanical properties of GaN nanostructures have not been explored in detail. The rigidity, thermal stability, and piezoelectric properties of GaN make it an interesting candidate for nano-electromechanical systems. We have fabricated doubly clamped GaN NW electromechanical resonators on sapphire using electron beam lithography and estimated the Young's modulus of GaN from resonance frequency measurements. For wires of triangular cross section with side ˜90 nm, we obtained values for the Young's modulus to be about 218 and 691 GPa, which are of the same order of magnitude as the values reported for bulk GaN. We also discuss the role of residual strain in the nanowire on the resonant frequency and the orientation dependence of the Young's modulus in wurtzite crystals.

  2. Fabrication of Si-based planar type patch clamp biosensor using silicon on insulator substrate

    International Nuclear Information System (INIS)

    The aim of this paper is to fabricate the planar type patch clamp ion-channel biosensor, which is suitable for the high throughput screening, using silicon-on-insulator (SOI) substrate. The micropore with 1.2 μm diameter is formed through the top Si layer and the SiO2 box layer of the SOI substrate by focused ion beam (FIB). Then the substrate is assembled into the microfluidic circuit. The human embryonic kidney 293 (HEK-293) cell transfected with transient receptor potential vanilloid type 1 (TRPV1) is positioned on the micropore and the whole-cell configuration is formed by the suction. Capsaicin is added to the extracellular solution as a ligand molecule, and the channel current showing the desensitization unique to TRPV1 is measured successfully

  3. Fabrication of Si-based planar type patch clamp biosensor using silicon on insulator substrate

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Z.L.; Asano, T. [Graduate University for Advanced Studies, Myodaiji, Okazaki, 444-8585 (Japan); Uno, H. [Institute for Molecular Science, Myodaiji, Okazaki, 444-8585 (Japan); Tero, R. [Graduate University for Advanced Studies, Myodaiji, Okazaki, 444-8585 (Japan); Institute for Molecular Science, Myodaiji, Okazaki, 444-8585 (Japan); Suzui, M.; Nakao, S. [Institute for Molecular Science, Myodaiji, Okazaki, 444-8585 (Japan); Kaito, T. [SII NanoTechnology Inc., 36-1, Takenoshita, Oyama-cho, Sunto-gun, Shizuoka, 410-1393 (Japan); Shibasaki, K.; Tominaga, M. [Okazaki Institute for Integrative Bioscience, 5-1, Higashiyama, Myodaiji, Okazaki, 444-8787 (Japan); Utsumi, Y. [Laboratory of Advanced Science and Technology for Industry, University of Hyogo, 3-1-2, Koto, Kamigori, Ako-gun, Hyogo, 678-1205 (Japan); Gao, Y.L. [Department of Physics and Astronomy, Rochester University, Rochester, New York 14627 (United States); Urisu, T. [Graduate University for Advanced Studies, Myodaiji, Okazaki, 444-8585 (Japan); Institute for Molecular Science, Myodaiji, Okazaki, 444-8585 (Japan)], E-mail: urisu@ims.ac.jp

    2008-03-03

    The aim of this paper is to fabricate the planar type patch clamp ion-channel biosensor, which is suitable for the high throughput screening, using silicon-on-insulator (SOI) substrate. The micropore with 1.2 {mu}m diameter is formed through the top Si layer and the SiO{sub 2} box layer of the SOI substrate by focused ion beam (FIB). Then the substrate is assembled into the microfluidic circuit. The human embryonic kidney 293 (HEK-293) cell transfected with transient receptor potential vanilloid type 1 (TRPV1) is positioned on the micropore and the whole-cell configuration is formed by the suction. Capsaicin is added to the extracellular solution as a ligand molecule, and the channel current showing the desensitization unique to TRPV1 is measured successfully.

  4. Harmonic Mitigated Front End Three Level Diode Clamped High Frequency Link Inverter by Using MCI Technique

    Directory of Open Access Journals (Sweden)

    Sreedhar Madichetty

    2014-02-01

    Full Text Available In this paper it proposes a high efficient soft-switching scheme based on zero-voltage-switching (ZVS and zero-current-switching(ZCS principle operated with a simple auxiliary circuit extended range for the front-end isolated DC-AC-DC-AC high power converter with an three phase three level diode clamped multi level inverter by using Minority Charge Carrier inspired optimization technique (MCI with Total Harmonic Distortion(THD,Switching losses, Selective harmonic elimination maintaining with its fundamental as an objective function. Input to the inverter is obtained by the photo voltaic cells and with battery bank. The switching scheme is optimized by MCI technique, analyzed and executed in Matlab and implemented with a digital signal processor (DSP .Experimental results with different loads have observed and shows its effectives, robustness of the applied technique.

  5. Note: Decoupling design for high frequency piezoelectric ultrasonic transducers with their clamping connections

    Energy Technology Data Exchange (ETDEWEB)

    Wang, F. J., E-mail: wangfujun@tju.edu.cn; Liang, C. M.; Tian, Y. L.; Zhao, X. Y.; Zhang, D. W. [Tianjin Key Laboratory of Equipment Design and Manufacturing Technology, School of Mechanical Engineering, Tianjin University, Tianjin 300072 (China); Zhang, H. J. [Tianjin Key Laboratory of Modern Mechatronics Equipment Technology, School of Mechanical Engineering, Tianjin Polytechnic University, Tianjin 300387 (China)

    2015-12-15

    This work presents the flexure-mechanism based decoupling design between high frequency piezoelectric ultrasonic transducers and their clamping connections to improve ultrasonic energy transmission efficiency. The ring, prismatic beam, and circular notched hinge based flanges were presented, and the crucial geometric dimensions of the transducers with the flexure decoupling flanges were determined. Finite element analysis (FEA) was carried out to investigate the dynamic characteristics of the transducers. Finally, experiments were conducted to examine and verify the effects of the proposed decoupling flanges. FEA and experimental results show that smaller frequency deviations and larger tip displacement amplitudes have been achieved by using the transducers with the flexure flanges compared with the transducer with a rigid ring-type flange, and thus the ultrasonic transmission efficiency can be improved through the flexure flanges.

  6. Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels.

    Science.gov (United States)

    Novak, Pavel; Gorelik, Julia; Vivekananda, Umesh; Shevchuk, Andrew I; Ermolyuk, Yaroslav S; Bailey, Russell J; Bushby, Andrew J; Moss, Guy W J; Rusakov, Dmitri A; Klenerman, David; Kullmann, Dimitri M; Volynski, Kirill E; Korchev, Yuri E

    2013-09-18

    Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton. The electrophysiology and ion-channel complement of far more abundant small synaptic terminals (≤ 1 μm) remain poorly understood. Here we report a method based on superresolution scanning ion conductance imaging of small synapses in culture at approximately 100-150 nm 3D resolution, which allows presynaptic patch-clamp recordings in all four configurations (cell-attached, inside-out, outside-out, and whole-cell). Using this technique, we report presynaptic recordings of K(+), Na(+), Cl(-), and Ca(2+) channels. This semiautomated approach allows direct investigation of the distribution and properties of presynaptic ion channels at small central synapses. PMID:24050398

  7. Multi-mode excitation of a clamped–clamped microbeam resonator

    KAUST Repository

    Younis, Mohammad I.

    2015-02-18

    We present modeling and simulation of the nonlinear dynamics of a microresonator subjected to two-source electrostatic excitation. The resonator is composed of a clamped–clamped beam excited by a DC voltage load superimposed to two AC voltage loads of different frequencies. One frequency is tuned close to the first natural frequency of the beam and the other is close to the third (second symmetric) natural frequency. A multi-mode Galerkin procedure is applied to extract a reduced-order model, which forms the basis of the numerical simulations. Time history response, Poincare’ sections, Fast Fourier Transforms FFT, and bifurcation diagrams are used to reveal the dynamics of the system. The results indicate complex nonlinear phenomena, which include quasiperiodic motion, torus bifurcations, and modulated chaotic attractors.

  8. A New Preparation of Pancreatic Ducts for Patch-Clamp Studies

    DEFF Research Database (Denmark)

    Novak, Ivana; Hug, M.J.

    1995-01-01

    -containing solutions. Duct cells were found to have a resting membrane voltage, Vm, of-55 ± 2 mV (n = 63). An increase in the bath K+ concentration (to 19 mmol/l) or an introduction of Ba2+ (3-5 mmol/l) into the bathing solution depolarized Vm by 16 and 22 mV (n = 21; 7), respectively. Secretin (10-9 mol/l) and...... parameters, monitored with the whole-cell patch-clamp method, and those in isolated perfused ducts, monitored with the conventional microelectrodes [Novak I, Greger R: Pflügers Arch 1988;411:58-68, 546-553], are comparable. Similar conclusion can be made about the calcium measurements [Hug M, et al: Pflügers...

  9. A DTC Algorithm for Induction Motor Drives with 3-level Diode-Clamped Inverters

    Directory of Open Access Journals (Sweden)

    M. Cirrincione

    2005-12-01

    Full Text Available This paper presents a novel Direct Torque Control (DTC algorithm for induction motor electrical drives supplied by 3-level diode clamped inverters. It is the natural extension of the classic Switching Table (ST based DTC, modified for a 3-level inverter supply. It presents also a very simple algorithm for the minimization of the capacitor voltage ripple. Results obtained with numerical simulations show that the employment of a 3-level inverter in such a control scheme permits to obtain the same dynamical performances as those obtained with a 2-level inverter with resulting lower torque and flux ripples as well as a lower harmonic content in the stator voltages and currents. Some considerations about the consequent benefits as for EMC of this drive are also presented.

  10. Single stage buck-boost DC-AC neutral point clamped inverter

    DEFF Research Database (Denmark)

    Mo, Wei; Loh, Poh Chiang; Andrew, A.;

    2012-01-01

    windings thus preventing the core from saturation; lowers the voltage stresses and power losses of inverter switches and reduces the sizes of filtering devices and as well as obtains better output performance compared to the original two-level Z-source inverters. A phase disposition pulse width modulation......This paper proposes a new single stage buck-boost DC-AC neutral point clamped inverter topology which integrates the cascaded configurations of recently introduced inductor-capacitor-capacitor-transformer impedance source network (by Adamowicz) and classic NPC configuration. As a consequence, it...... has enhanced buck-boost functionality and low output voltage distortions compared to the traditional Z-source inverter; it has continuous input current which reduces the source stress and inverter noise; it also contains two built-in capacitors which can block the DC current in the transformer...

  11. Pulse-Width-Modulation of Neutral-Point-Clamped Sparse Matrix Converter

    DEFF Research Database (Denmark)

    Loh, P.C.; Blaabjerg, Frede; Gao, F.;

    2007-01-01

    Sparse matrix converter is an alternative "allsemiconductor" energy processor proposed recently for converting an ac source with fixed magnitude and frequency to a variable voltage and frequency supply that can meet the requirements of a particular industry application. In principle, sparse matrix...... input current and output voltage can be achieved with minimized rectification switching loss, rendering the sparse matrix converter as a competitive choice for interfacing the utility grid to (e.g.) defense facilities that require a different frequency supply. As an improvement, sparse matrix converter...... literature. Addressing the afore-described issues, this paper focuses on the operational mode analysis of a three-level sparse matrix converter implemented using a neutral-point-clamped inversion stage, and  the design of a number of PWM and modulation ratio compensation schemes for controlling the converter...

  12. Comparative Evaluation of Pulsewidth Modulation Strategies for Z-Source Neutral-Point-Clamped Inverter

    DEFF Research Database (Denmark)

    Loh, P.C.; Blaabjerg, Frede; Wong, C.P.

    2007-01-01

    modulation (PWM) strategies for controlling the Z-source NPC inverter. While developing the PWM techniques, attention has been devoted to carefully derive them from a common generic basis for improved portability, easier implementation, and most importantly, assisting readers in understanding all concepts......Z-source neutral-point-clamped (NPC) inverter has recently been proposed as an alternative three-level buck-boost power conversion solution with an improved output waveform quality. In principle, the designed Z-source inverter functions by selectively "shooting through" its power sources, coupled...... to the inverter using two unique Z-source impedance networks, to boost the inverter three-level output waveform. Proper modulation of the new inverter would therefore require careful integration of the selective shoot-through process to the basic switching concepts to achieve maximal voltage...

  13. Ultra-coherent nanomechanical resonators via soft clamping and dissipation dilution

    CERN Document Server

    Tsaturyan, Yeghishe; Polzik, Eugene S; Schliesser, Albert

    2016-01-01

    The small mass and high coherence of nanomechanical resonators render them the ultimate force probe, with applications ranging from biosensing and magnetic resonance force microscopy, to quantum optomechanics. A notorious challenge in these experiments is thermomechanical noise related to dissipation through internal or external loss channels. Here, we introduce a novel approach to defining nanomechanical modes, which simultaneously provides strong spatial confinement, full isolation from the substrate, and dilution of the resonator material's intrinsic dissipation by five orders of magnitude. It is based on a phononic bandgap structure that localises the mode, without imposing the boundary conditions of a rigid clamp. The reduced curvature in the highly tensioned silicon nitride resonator enables mechanical $Q>10^{8}$ at $ 1 \\,\\mathrm{MHz}$, yielding the highest mechanical $Qf$-products ($>10^{14}\\,\\mathrm{Hz}$) yet reported at room temperature. The corresponding coherence times approach those of optically t...

  14. Simple Switching Strategy for High-Torque Control Performance utilizing Neutral Point Clamped Multilevel Inverter

    Directory of Open Access Journals (Sweden)

    Nor Faezah Alias

    2013-12-01

    Full Text Available Three-level Neutral Point Clamped (NPC inverter allows the configuration of switching devices to operate at high voltage and produce lower current/voltage harmonics. It is known that, DTC of induction machine which employs hysteresis controller has major drawbacks namely larger torque ripple and variable switching frequency. This paper aims to propose a suitable voltage vector selection to provide better torque regulation and lower switching frequency by employing DTC with 3-level NPC multilevel inverter. A simple switching strategy was formulated using 7-level torque hysteresis and 2-level flux hysteresis controllers to give more options in selecting an appropriate voltage vector, inherently, according the motor operation conditions. The improvements offered were verified through simulations.

  15. Influence of pipe wall effects on clamp-on ultrasonic flow measurement

    Energy Technology Data Exchange (ETDEWEB)

    Funck, B.; Mitzkus, A. [Flexim GmbH, Berlin (Germany); Panicke, M.; Specht, O.; Fiedler, O. [Flexim GmbH, Berlin (Germany)]|[Rostock Univ. (Germany). FB Elektrotechnik

    2001-07-01

    Clamp-on ultrasonic flowmeters enable the noninvasive measurement of flow in pipes. The transducers are coupled to the pipe wall and cover a wide range of rated inside diameters when suitably dimensioned. The transit time technique is applicable at sufficiently pure fluids. The time difference of contrapropagating signals is related to the velocity of flow on the path defined by the transducers. The Doppler method utilizes the signals scattered by particles or bubbles. The calibration factor depends on the size and position of the volume determined by the intersection of the transducer beams. The paper reports on effects of the pipe wall on both the transit time technique and the Doppler method of flow measurement. The sound propagation between the transducer is described by acoustic transfer functions. (orig.)

  16. Multifrequency excitation of a clamped–clamped microbeam: Analytical and experimental investigation

    KAUST Repository

    Jaber, Nizar

    2016-03-14

    Using partial electrodes and a multifrequency electrical source, we present a large-bandwidth, large-amplitude clamped–clamped microbeam resonator excited near the higher order modes of vibration. We analytically and experimentally investigate the nonlinear dynamics of the microbeam under a two-source harmonic excitation. The first-frequency source is swept around the first three modes of vibration, whereas the second source frequency remains fixed. New additive and subtractive resonances are demonstrated. We illustrated that by properly tuning the frequency and amplitude of the excitation force, the frequency bandwidth of the resonator is controlled. The microbeam is fabricated using polyimide as a structural layer coated with nickel from the top and chromium and gold layers from the bottom. Using the Galerkin method, a reduced order model is derived to simulate the static and dynamic response of the device. A good agreement between the theoretical and experimental data are reported.

  17. Calculation Method for Flight Limit Load of V-band Clamp Separation Shock

    Science.gov (United States)

    Iwasa, Takashi; Shi, Qinzhong

    A simplified calculation method for estimating a flight limit load of the V-band clamp separation shock was established. With this method, the flight limit load is estimated through addition of an appropriate envelope margin to the results acquired with the simplified analysis method proposed in our previous paper. The envelope margin used in the method was calculated based on the reviews on the differences observed between the results of a pyroshock test and the analysis. Using the derived envelope margin, a calculating formula of the flight limit load, which envelopes the actual pyroshock responses with a certain probability, was developed. Based on the formula, flight limit loads for several actual satellites were estimated and compared to the test results. The comparative results showed that the estimated flight limit loads appropriately envelope the test results, which confirmed the effectiveness of the proposed method.

  18. Model Predictive Control of Z-source Neutral Point Clamped Inverter

    DEFF Research Database (Denmark)

    Mo, Wei; Loh, Poh Chiang; Blaabjerg, Frede

    2011-01-01

    inductor current, capacitor voltage as well as switching frequency, transient response are all regulated as subjecting to constraints of this control method. The quality of output waveform, stability of impedance-network, level constraint of variable switching frequency as well as robustness of transient......This paper presents Model Predictive Control (MPC) of Z-source Neutral Point Clamped (NPC) inverter. For illustration, current control of Z-source NPC grid-connected inverter is analyzed and simulated. With MPC’s advantage of easily including system constraints, load current, impedance network...... response are obtained at the same time with a formulated Z-source NPC inverter network model. Operation steady state and transient state simulation results of MPC are going to be presented, which shows good reference tracking ability of this method. It provides new control method for Z-source NPC inverter...

  19. Direct-conversion switching-mode audio power amplifier with active capacitive voltage clamp

    DEFF Research Database (Denmark)

    Ljusev, Petar; Andersen, Michael Andreas E.

    2005-01-01

    This paper discusses the advantages and problems when implementing direct energy conversion switching-mode audio power amplifiers. It is shown that the total integration of the power supply and Class D audio power amplifier into one compact direct converter can simplify the design, increase effic...... efficiency, reduce the product volume and lower its cost. As an example, the principle of operation and the measurements made on a direct-conversion switching-mode audio power amplifier with active capacitive voltage clamp are presented.......This paper discusses the advantages and problems when implementing direct energy conversion switching-mode audio power amplifiers. It is shown that the total integration of the power supply and Class D audio power amplifier into one compact direct converter can simplify the design, increase...

  20. Comparative Analysis and Simulation of Diode Clamped & Cascaded H-Bridge Multilevel Inverter using SPWM Technique

    Directory of Open Access Journals (Sweden)

    Mohit Jain

    2015-01-01

    Full Text Available Multilevel inverters have become more popular over the years in high power medium voltage applications without the use of a transformer and with promise of less disturbance & reduced harmonic distortion. In this paper, two types of multilevel converter in three phase configuration, cascaded H-Bridge multilevel inverter (CMLI and diode clamped multilevel inverter (DCMLI of 5 and 7-level are modelled and compared in the case of feeding of a three phase squirrel cage induction motor. Here, carrier based sinusoidal pulse width modulation (SPWM technique is used as the modulation strategy. These modulation strategy include phase disposition technique (PD, phase opposition disposition technique (POD, and an alternative phase opposition disposition technique (APOD. A detailed study of the modulation technique has been carried out through MATLAB/SIMULINK for both multilevel converters and a comparative evaluation between DCMLI and CMLI using SPWM technique in terms of THD%.

  1. The strain dependence of the transport current characteristics in Bi-2223 silver clamped thick film

    International Nuclear Information System (INIS)

    The dependences of stress on strain, strain on the critical current density, and the relation of Jc(H) at several strains were measured in Bi-2223 silver clamped thick film. The results indicate that the stress-strain curve has two strain transitions which are respectively correspond to the beginning and ending of breaking in Bi1.8Pb0.4Sr2Ca2Cu3Oy thick film. At ΔX/X≤2.5%, the critical current density Jc decreases slowly with ΔX/X increasing. At ΔX/X≥2.5%, Jc decreases rapidly with ΔX/X increasing. As strain increases, the characteristics of Jc(H) decrease rapidly and the weak link junctions emerges obviously. The microstructures show that the decrease of Jc and the variance of characteristics of Jc(H) relate to the breaking of intergrain and grains in the thick film

  2. The fluorescent detection of Paradiplozoon sp. (Monogenea: Diplozoidae attachment clamps' sclerites and integumental proteins : research communication

    Directory of Open Access Journals (Sweden)

    S.J. Milne

    2006-09-01

    Full Text Available In the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 µl WGA-TRX, 5 µl SYTO 9 and 5 µl of Cal co fluor White M2R. Rhodamine, Fluorescein and DAPI bandpass filters on the microscope enabled selective light wavelength illumination of the three flourochromes by a mercury light source. This method provided an easy and rapid methodology to show the internal sclerites of attachment clamps. It is suitable for alcohol preserved specimens and may have additional applications in other helminth organisms.

  3. Palaeoclimate Interpretation at the Locality Velka Causa (Early Miocene, Western Carpathians) based on CLAMP Method

    International Nuclear Information System (INIS)

    Palaeontological locality Velka Causa is situated in the Handlova-Novaky brown-coal basin (Upper Nitra depression) about 3 km north of Velka Causa in an outcrop exposed by a stream flowing through the village of Mala Causa. Studied palaeobotanical material, stored in the Hornonitrianske museum in Prievidza, is fossilized in light-gray clays of Eggenburgian age proved by occurrence of molluscs, foraminifers and ostracods. Sediments were laid down in a sea gulf formed during an Eggenburgian transgression. Plant imprints belong to plants which grew near the sea shore and on the slopes of adjacent mountains. Only dicots were chosen from all fossil leaves occurred in the studied collection. They were sorted into morpho-species and were examined for the presence or absence of each of the 31 morphological characters used after CLAMP categorization. A total of 246 fossil samples were studied. Vegetation used in the dataset for this study indicates subtropical climate.

  4. A Color LED Driver Implemented by the Active Clamp Forward Converter

    Directory of Open Access Journals (Sweden)

    C. H. Chang

    2013-03-01

    Full Text Available Because light emitting diodes (LEDs have the advantages of dc working voltage, high luminescent efficiency, shortignition time, high reliability and pollution free, they have substituted for incandescent bulbs and fluorescent lampsgradually. In order to simplify circuit complexity, an active clamp forward converter with the sequential color display(SCD control is proposed to drive red, green and blue (RGB LED arrays. The proposed converter has zero-voltageswitching (ZVS operations of both the main switch and the auxiliary switch, resulting in high system efficiency. DrivingRGB LED arrays sequentially by one converter can save components and reduce cost significantly. Additionally, thepulse-width modulation (PWM control is applied to achieve a large chromaticity variation. The circuit operations areanalyzed in detail and the circuit parameters are designed based on the practical considerations. Finally, an illustrativeexample is implemented to demonstrate the feasibility and validity of the proposed LED driver.

  5. Room temperature, very sensitive thermometer using a doubly clamped microelectromechanical beam resonator for bolometer applications

    Science.gov (United States)

    Zhang, Y.; Watanabe, Y.; Hosono, S.; Nagai, N.; Hirakawa, K.

    2016-04-01

    We propose a room temperature, all electrical driving and detecting, very sensitive thermometer structure using a microelectromechanical (MEMS) resonator for bolometer applications. We have fabricated a GaAs doubly clamped MEMS beam resonator whose oscillation can be excited and detected by the piezoelectric effect. When a heating power is applied to a NiCr film deposited on the MEMS beam surface, internal thermal stress is generated in the beam, leading to a reduction in the resonance frequency. The present device detects the shift in the resonance frequency caused by heating and works as a very sensitive thermometer. When the resonator was driven by a voltage slightly below the threshold for the nonlinear, hysteretic oscillation, the thermometer showed a voltage responsivity of about 3300 V/W, while keeping a low noise spectral density of about 60 nV/Hz1/2, demonstrating a noise equivalent power of bolometers for room-temperature operation.

  6. Characterization of a patch-clamp microchannel array towards neuronal networks analysis

    DEFF Research Database (Denmark)

    Alberti, Massimo; Snakenborg, Detlef; Lopacinska, Joanna M.;

    2010-01-01

    simultaneous patch clamping of cultured cells or neurons in the same network. A disposable silicon/silicon dioxide (Si/SiO2) chip with a microhole array was integrated in a microfluidic system for cell handling, perfusion and electrical recording. Fluidic characterization showed that our PC mu CA can work as a...... theory, but the capacitance is still too high for high resolution recording. The system was tested on HeLa cells: successful cell trapping with a sealing of 40 M Omega was recorded. Modification of the Si/SiO2 chip is needed in order to achieve a better sealing and long-term cell culturing in the PC mu...

  7. Five-Level Z-Source Neutral Point-Clamped Inverter

    DEFF Research Database (Denmark)

    Gao, F.; Loh, P.C.; Blaabjerg, Frede;

    2007-01-01

    This paper proposes a five-level Z-source neutralpoint- clamped (NPC) inverter with two Z-source networks functioning as intermediate energy storages coupled between dc sources and NPC inverter circuitry. Analyzing the operational principles of Z-source network with partial dclink shoot......-through scheme reveals the hidden theories in the five-level Z-source NPC inverter unlike the operational principle appeared in the general two-level Z-source inverter, so that the five-level Z-source NPC inverter can be designed with the modulation of carrier-based phase disposition (PD) or alternative phase...... opposite disposition (APOD) technique. To verify the theoretical findings and practical issues, a scaled down laboratory prototype was constructed and tested with a 1.3 kw induction motor load....

  8. Gel chromatography of immunoextracted plasma calcitonin in response to the calcium clamp in healthy males

    International Nuclear Information System (INIS)

    A radioimmunoassay (RIA) was developed for immunoreactive calcitonin (iCT) in human plasma. Antibodies against synthetic human calcitonin (hCT) coupled to bovine serum albumin (BSA) were raised in rabbits and were directed against the carboxy terminal part of CT. The detection limit of the assay was 8 pg/ml. In 7 males the iCT response to a calcium-clamp was studied. Blood was collected at 0,30 and 60 min after the start of the calcium infusion. iCT was measured directly in plasma and in extracts obtained after purification of plasma iCT by means of immobilized CT antiboides. There was a good correlation between iCT in plasma samples and extracts, r = 0.993, n = 14 (P< 0.001). Dilution curves of extracts and plasma were parallel with the hCT standard curves. Gel chromatography of the extracts on Sephadex G-50 and G-75 disclosed heterogeneity of iCT in normal plasma during basal conditions as well as during calcium stimulation. Thirty min after the start of the calcium clamp all molecular forms, most likely constituting monomeric and dimeric CT and larger forms, were increased, while after 60 min iCT seemed to constitute predominantly forms larger than monomeric TCY. Basal levels of unextracted iCT in healthy males (n = 44, 37 +/- 10 years) were 15 +/- 9 pg-equivalents/ml (mean +/- SD), which was higher than in females (n = 40, 32 +/- 9 years) 11 +/- 4 pg-equivalents/ml (P < 0.05). (author)

  9. Management of open olecranon fractures using clamp-cum-compressor device

    Directory of Open Access Journals (Sweden)

    Kundu Zile

    2009-01-01

    Full Text Available Background: Open fractures of olecranon are not a rare occurrence in patients with road traffic accidents particularly motor bike riders who don′t use elbow guards. Definitive treatment has to be delayed in many till the wound heals. The present study was conducted to evaluate the results of open fractures of olecranon using clamp-cum-compressor device. Materials and Methods: Seventeen patients between the ages of 20 and 45 years of open olecranon fractures reported 5-20 days after injury were treated using an indigenous clamp-cum-compressor. All fractures were Mayo type II-A, i.e., displaced, stable and noncomminuted. Four patients had Gustilo-Anderson grade I and 13 had Gustilo-Anderson grade II open fractures. The patients with transverse or short oblique fractures were included in the study. The apparatus was applied under regional anesthesia after thorough washing and debridement of wounds with few loose sutures applied wherever needed. The wounds healed within 2-4 weeks and fractures united within 8-10 weeks. The elbow was mobilized with apparatus still in place. The results were evaluated by MayoElbow performance score. Results: We achieved excellent results in twelve patients, good in four and poor in one patient, who reported late, hooks of the apparatus were cut through the proximal fragment, leading to union of fracture in elongation and restricted elbow movements. Conclusion: The apparatus was found to be quite useful in transverse and short oblique fractures with contamination or infection, where internal fixation has to be delayed or avoided.

  10. Dynamic response of a clamped/free hollow circular cylinder under travelling torsional impact loads

    International Nuclear Information System (INIS)

    Impact-induced vibrations in the casing of a gas centriguge due to a sudden failure of the spinning rotor (crash) can cause structural disintegrity of the casing. In order to study the influence of the rotor failure bahaviour and the impact load histories on the dynamic response of the casing, a simple crash model is proposed in this paper to analyse the transient torsional response due to tangential components of the impact loads. The casing is modeled as a linear-elastic hollow circular cylinder, clamped at the lower end and free at the upper end. The rotor is thought to breakup in identical sections in a sequence determined by its fracture behaviour. Each section is assumed to cause an axi-symmetric load distribution at the inner surface of the casing. Therefore the problem is essentially reduced to the analysis of a clamped/free cylinder under travelling torsional impact loads. The problem is solved by representing the impact loads as local pulses acting over the length of the sections. A perturbation method is used to show that the general two-dimensional theory of axi-symmetric torsional wave propagation in circular cylinders, for the problem under consideration, may be approximated by the elementary one-dimensional theory. Solutions are obtained according to the usual modal expansion approach. Measurements of transient torsional responses are shown to be in good agreement with the calculated responses by choosing a suitable shape of the pulses. The effects of travelling velocity and pulse shape are investigated. Finally the transfer of kinetic energy in the rotor to vibrational energy of torsion in the casing is studied. (orig.)

  11. MATLAB-based automated patch-clamp system for awake behaving mice.

    Science.gov (United States)

    Desai, Niraj S; Siegel, Jennifer J; Taylor, William; Chitwood, Raymond A; Johnston, Daniel

    2015-08-01

    Automation has been an important part of biomedical research for decades, and the use of automated and robotic systems is now standard for such tasks as DNA sequencing, microfluidics, and high-throughput screening. Recently, Kodandaramaiah and colleagues (Nat Methods 9: 585-587, 2012) demonstrated, using anesthetized animals, the feasibility of automating blind patch-clamp recordings in vivo. Blind patch is a good target for automation because it is a complex yet highly stereotyped process that revolves around analysis of a single signal (electrode impedance) and movement along a single axis. Here, we introduce an automated system for blind patch-clamp recordings from awake, head-fixed mice running on a wheel. In its design, we were guided by 3 requirements: easy-to-use and easy-to-modify software; seamless integration of behavioral equipment; and efficient use of time. The resulting system employs equipment that is standard for patch recording rigs, moderately priced, or simple to make. It is written entirely in MATLAB, a programming environment that has an enormous user base in the neuroscience community and many available resources for analysis and instrument control. Using this system, we obtained 19 whole cell patch recordings from neurons in the prefrontal cortex of awake mice, aged 8-9 wk. Successful recordings had series resistances that averaged 52 ± 4 MΩ and required 5.7 ± 0.6 attempts to obtain. These numbers are comparable with those of experienced electrophysiologists working manually, and this system, written in a simple and familiar language, will be useful to many cellular electrophysiologists who wish to study awake behaving mice. PMID:26084901

  12. seismic-py: Reading seismic data with Python

    Directory of Open Access Journals (Sweden)

    2008-08-01

    Full Text Available The field of seismic exploration of the Earth has changed
    dramatically over the last half a century. The Society of Exploration
    Geophysicists (SEG has worked to create standards to store the vast
    amounts of seismic data in a way that will be portable across computer
    architectures. However, it has been impossible to predict the needs of the
    immense range of seismic data acquisition systems. As a result, vendors have
    had to bend the rules to accommodate the needs of new instruments and
    experiment types. For low level access to seismic data, there is need for a
    standard open source library to allow access to a wide range of vendor data
    files that can handle all of the variations. A new seismic software package,
    seismic-py, provides an infrastructure for creating and managing drivers for
    each particular format. Drivers can be derived from one of the known formats
    and altered to handle any slight variations. Alternatively drivers can be
    developed from scratch for formats that are very different from any previously
    defined format. Python has been the key to making driver development easy
    and efficient to implement. The goal of seismic-py is to be the base system
    that will power a wide range of experimentation with seismic data and at the
    same time provide clear documentation for the historical record of seismic
    data formats.

  13. Enhancing the seismic capability of the on-power refueling system of the CANDU reactor

    International Nuclear Information System (INIS)

    The CANDU reactor assembly includes several hundred horizontal fuel channels, each containing twelve fuel bundles, arranged in a square lattice, and supported by the reactor structures. CANDU operates on natural uranium or other low fissile content fuel, and is refueled on-power, with either four or eight fuel bundles in a channel being replaced during each refueling operation. The fueling machines clamp onto the opposite ends of the fuel channel to be refueled. The seismic capacity of this refueling system is evaluated in terms of its dynamic response during an earthquake. This paper describes the approach adopted to enhance the seismic capability of the fueling machine and calandria assembly for earthquakes of O.3g ground acceleration covering a broad range of soil conditions ranging from soft to hard. A detailed, 3-D finite element seismic model of the fueling machine and calandria assembly system is developed to calculate the seismic responses of the structure. Some relatively simple hardware design changes have been considered to increase the seismic capacity of the CANDU 6 reactor. These changes in the fueling machine and calandria assembly of the CANDU 6 reactor are briefly described. They have been incorporated into the finite element seismic model of the system. Most of these design changes have already been considered and implemented in other CANDU reactor projects. The current CANDU 6 reactor design fully meets the requirements of seismic qualification for sites with potential for O.2g ground acceleration where the seismic loads need to be combined with the other design loads for the support and pressure boundary components to demonstrate compliance with the applicable Code requirements. In the present study it is demonstrated that, with relatively simple hardware changes, the fueling machine and calandria assembly of the CANDU 6 reactor can withstand earthquakes of O.3g ground acceleration. Based on the current study and some preliminary analysis of the

  14. New systems of seismic foundation isolation for spatial seismic excitation

    International Nuclear Information System (INIS)

    Mathematical calculations are presented of the behavior of seismic isolators of the founding, using shear deformation of rubber layers and sliding motion in the contact planes with a low shear friction coefficient. Also studied is the vertical seismic motion which also contributes to the stress in structural elements and to the behavior of vibration isolation elements. The results are presented of laboratory tests of isolating elements as well as graphs of the harmonic seismic response of prestressed concrete column models. (M.D.) 4 figs., 2 tabs., 5 refs

  15. Updated Colombian Seismic Hazard Map

    Science.gov (United States)

    Eraso, J.; Arcila, M.; Romero, J.; Dimate, C.; Bermúdez, M. L.; Alvarado, C.

    2013-05-01

    The Colombian seismic hazard map used by the National Building Code (NSR-98) in effect until 2009 was developed in 1996. Since then, the National Seismological Network of Colombia has improved in both coverage and technology providing fifteen years of additional seismic records. These improvements have allowed a better understanding of the regional geology and tectonics which in addition to the seismic activity in Colombia with destructive effects has motivated the interest and the need to develop a new seismic hazard assessment in this country. Taking advantage of new instrumental information sources such as new broad band stations of the National Seismological Network, new historical seismicity data, standardized global databases availability, and in general, of advances in models and techniques, a new Colombian seismic hazard map was developed. A PSHA model was applied. The use of the PSHA model is because it incorporates the effects of all seismic sources that may affect a particular site solving the uncertainties caused by the parameters and assumptions defined in this kind of studies. First, the seismic sources geometry and a complete and homogeneous seismic catalog were defined; the parameters of seismic rate of each one of the seismic sources occurrence were calculated establishing a national seismotectonic model. Several of attenuation-distance relationships were selected depending on the type of seismicity considered. The seismic hazard was estimated using the CRISIS2007 software created by the Engineering Institute of the Universidad Nacional Autónoma de México -UNAM (National Autonomous University of Mexico). A uniformly spaced grid each 0.1° was used to calculate the peak ground acceleration (PGA) and response spectral values at 0.1, 0.2, 0.3, 0.5, 0.75, 1, 1.5, 2, 2.5 and 3.0 seconds with return periods of 75, 225, 475, 975 and 2475 years. For each site, a uniform hazard spectrum and exceedance rate curves were calculated. With the results, it is

  16. Seismicity and tectonics of Bangladesh

    International Nuclear Information System (INIS)

    Northern and eastern Bangladesh and surrounding areas belong to a seismically active zone and are associated with the subduction of the Indian plate. The seismicity and tectonics have been studied in detail and the observations have been correlated to understand the earthquake phenomenon in the region. The morphotectonic behaviour of northern Bangladesh shows that it is deeply related to the movement of the Dauki fault system and relative upliftment of the Shillong plateau. Contemporary seismicity in the Dauki fault system is relatively quiet comparing to that in the Naga-Disang-Haflong thrust belt giving rise to the probability of sudden release of energy being accumulated in the vicinity of the Dauki fault system. This observation corresponds with the predicted average return period of a large earthquake (1897 type) and the possibility of M > 8 earthquake in the vicinity of the Dauki fault within this century should not be ruled out. The seismicity in the folded belt in the east follows the general trend of Arakan-Yoma anticlinorium and represents shallow and low-angled thrust movements in conformity with the field observation. Seismotectonic behaviour in the deep basin part of Bangladesh demonstrates that an intraplate movement in the basement rock has been taking place along the deep-seated faults causing relative upliftment and subsidence in the basin. Bangladesh has been divided into three seismic zones on the basis of morphotectonic and seismic behaviour. Zone-I has been identified as the zone of high seismic risk. (author). 43 refs, 5 figs, 3 tabs

  17. Seismic data formats, archival and exchange

    Czech Academy of Sciences Publication Activity Database

    Dost, B.; Zedník, Jan; Havskov, J.; Willemann, R.; Bormann, P.

    Vol. 1. Potsdam : GeoForschungsZentrum, 2002 - (Bormann, P.), s. 10.1-10.20 ISBN 3-9808780-0-7 Institutional research plan: CEZ:AV0Z3012916 Keywords : seismic data * exchange of seismic data * seismic network * seismic observatories Subject RIV: DC - Siesmology, Volcanology, Earth Structure

  18. A High-Resolution Record of Early Miocene Antarctic Glacial History From Downhole Logs, Site 1165, ODP Leg 188

    Science.gov (United States)

    Williams, T.; Barr, S.; Handwerger, D.

    2002-12-01

    ODP Site 1165, located 400 km NW of Prydz Bay, contains the most detailed record yet obtained of Early Miocene Antarctic glacial history. The 520-m-thick Early Miocene consists of dark grey claystone with silt laminae (contourites) alternating with decimeter-scale layers of greenish-grey bioturbated claystone that contain common ice rafted debris (IRD). These sediments are interpreted to record pulses of IRD from the continent, and alternations in the position or strength of the currents at the site. Downhole logs of electrical resistivity, natural gamma radiation, and density also record the alternations between the two facies. Here we assess the lithological significance of the logs by examining the opal, IRD, and susceptibility of two short intervals at high resolution, with samples taken every 10 cm, or about 1250 yr. The IRD-bearing greenish-grey claystone corresponds to higher resistivity and density because of increased cementation by silica, and corresponds to lower natural gamma radiation because of decreased clay content. Thus, using the logs, lithology can be inferred for intervals where no core was recovered. Based mainly on magnetostratigraphy, the age of the base of the hole is 22 Ma, and the sedimentation rate for the Early Miocene about 9 cm/kyr. The regular spacing of the facies alternations in the logs suggests they are astronomically paced. Between 18-20 Ma, the main periodicity is in the precessional range, and between 20-22 Ma, the logs have a saw-toothed form, with a major peak in IRD at the beginning of each, repeating about every 100 kyr. We think that the IRD pulses represent deglaciations. Hence our data suggest that the Antarctic continent, at least around the Prydz Bay region, was glaciated in the Early Miocene, and that the size of the ice sheet oscillated on Milankovitch time scales.

  19. Integrated system for seismic evaluations

    International Nuclear Information System (INIS)

    This paper describes the various features of the Seismic Module of the CARES system (Computer Analysis for Rapid Evaluation of Structures). This system was developed by Brookhaven National Laboratory (BNL) for the US Nuclear Regulatory Commission to perform rapid evaluations of structural behavior and capability of nuclear power plant facilities. The CARES is structured in a modular format. Each module performs a specific type of analysis i.e., static or dynamic, linear or nonlinear, etc. This paper describes the features of the Seismic Module in particular. The development of the Seismic Module of the CARES system is based on an approach which incorporates all major aspects of seismic analysis currently employed by the industry into an integrated system that allows for carrying out interactively computations of structural response to seismic motions. The code operates on a PC computer system and has multi-graphics capabilities. It has been designed with user friendly features and it allows for interactive manipulation of various analysis phases during the seismic design process. The capabilities of the seismic module include (a) generation of artificial time histories compatible with given design ground response spectra, (b) development of Power Spectral Density (PSD) functions associated with the seismic input, (c) deconvolution analysis using vertically propagating shear waves through a given soil profile, and (d) development of in-structure response spectra or corresponding PSD's. It should be pointed out that these types of analyses can also be performed individually by using available computer codes such as FLUSH, SAP, etc. The uniqueness of the CARES, however, lies on its ability to perform all required phases of the seismic analysis in an integrated manner. 5 refs., 6 figs

  20. Seismic Solvability Problems

    CERN Document Server

    Lau, August

    2012-01-01

    Classical approach of solvability problem has shed much light on what we can solve and what we cannot solve mathematically. Starting with quadratic equation, we know that we can solve it by the quadratic formula which uses square root. Polynomial is a generalization of quadratic equation. If we define solvability by using only square roots, cube roots etc, then polynomials are not solvable by radicals (square root, cube root etc). We can classify polynomials into simple (solvable by radicals) and complex (not solvable by radicals). We will use the same metaphor to separate what is solvable (simple part) and what is not solvable (complex part). This paper is a result of our presentation at a University of Houston seminar. In this paper, we will study seismic complexity through the eyes of solvability. We will investigate model complexity, data complexity and operator complexity. Model complexity is demonstrated by multiple scattering in a complex model like Cantor layers. Data complexity is studied through Bet...