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Sample records for citocinina bap retrasa

  1. Multiplicação in vitro da ameixeira 'Santa Rosa': efeito da citocinina BAP

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    Rogalski Marcelo

    2003-01-01

    Full Text Available A ameixeira 'Santa Rosa' apresenta alta produtividade, ótimo sabor e aparência dos frutos para a comercialização. No entanto, por ser altamente suscetível à escaldadura das folhas (Xylella fastidiosa Wells, esta variedade apresenta problemas de cultivo no sul do Brasil. As técnicas de cultura in vitro permitem propagar e rapidamente espécies de interesse, além de permitir a limpeza de patógenos e a produção de matrizes com qualidade genética e sanitária comprovada. Porém, o uso prático da propagação in vitro requer a otimização das condições de cultura para cada espécie e/ou variedade. Dentre os fatores que mais influenciam a micropropagação, estão as citocininas, com destaque para o BAP. O objetivo principal deste trabalho foi avaliar o potencial de multiplicação in vitro da ameixeira 'Santa Rosa' sob diferentes concentrações de BAP. Após três subculturas em meio MA1, segmentos nodais com 0,5-1,0 cm foram submetidos a diferentes concentrações de BAP (0,5; 1,0; 2,0 e 3,0 mg.L-1. Os resultados mostraram que não ocorreram diferenças significativas no número de brotos para as diferentes concentrações de BAP testadas. No entanto, o maior número de brotos por explante (3,6 obteve-se na concentração de 2,0 mg.L-1 e a maior altura média dos brotos foi obtido na concentração de 0,5 mg.L-1 de BAP. Concentrações maiores que a 0,5 mg.L-1 de BAP inibiram o crescimento dos brotos. A micropropagação da ameixeira 'Santa Rosa' a partir de ápices caulinares e gemas laterais em meio de cultura MA1 mostrou-se eficaz.

  2. BAP1 and Cancer

    OpenAIRE

    Carbone, Michele; Yang, Haining; Pass, Harvey I.; Krausz, Thomas; Joseph R. Testa; Gaudino, Giovanni

    2013-01-01

    BAP1 is a deubiquitylase that is found associated with multi-protein complexes that regulate key cellular pathways, including the cell cycle, cellular differentiation, cell death, gluconeogenesis and the DNA damage response (DDR). Recent findings indicate that germline BAP1 mutations cause a novel cancer syndrome, characterized, at least in the affected families studied so far, by the onset at an early age of benign melanocytic skin tumours with mutated BAP1, and later in life by a high incid...

  3. Indução de brotações em explantes de segmentos de folhas de plântulas de urucueiro em diferentes citocininas Induction of shoot buds in leaf explants of annatto seedlings in differents cytokinins

    OpenAIRE

    Jacqueline Leite Almeida; Francisco Célio Guedes Almeida; Raimundo de Pontes Nunes; Francisco Aécio Guedes Almeida

    1996-01-01

    Segmentos de folhas de plântulas de urucum (Bixa orellana L), foram cultivados in vitro, em um meio básico idealizado por Murashige & Skoog (1962), suplementado com 0,5mg/l dos reguladores do crescimento, benzilaminopurina (BAP), cinetina (KN) e isopentenil-adenina (2iP), isolados ou combinados entre si, com o objetivo de avaliar qual a melhor citocinina ou combinação destas para indução de brotações de boa qualidade visando à micropropagação. Após 60 dias de cultivo, as gemas obtidas foram t...

  4. Indução de brotações em explantes de segmentos de folhas de plântulas de urucueiro em diferentes citocininas Induction of shoot buds in leaf explants of annatto seedlings in differents cytokinins

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    Jacqueline Leite Almeida

    1996-04-01

    Full Text Available Segmentos de folhas de plântulas de urucum (Bixa orellana L, foram cultivados in vitro, em um meio básico idealizado por Murashige & Skoog (1962, suplementado com 0,5mg/l dos reguladores do crescimento, benzilaminopurina (BAP, cinetina (KN e isopentenil-adenina (2iP, isolados ou combinados entre si, com o objetivo de avaliar qual a melhor citocinina ou combinação destas para indução de brotações de boa qualidade visando à micropropagação. Após 60 dias de cultivo, as gemas obtidas foram transferidas para um meio MS modificado, contendo a metade da formulação de sais minerais, sem a suplementação de reguladores do crescimento. Gemas de melhor qualidade foram obtidas nos tratamentos onde a KN e o BAP estavam em combinação. A KN sozinha não foi eficiente na indução de brotações. O maior número de gemas por explante foi obtido com KN, BAP e 2iP e no tratamento com apenas BAP.Excised leaf explants of annatto (Bixa orellana L. seedlings were cultivated on a Murashige & Skoog basal medium containing benzylaminopurine (BAP, kinetin (KN and isopentenyl-adenine (2iP individually or in combination, each at concentration of 0.5mg/l, with the purpose of evaluated the best cytokinins or cytokinins combination for shoot bud formation. After 60 days, regenerated shoots were transferred to half strenght MS medium without growth regulators. Best shoots were observed where KN and BAP were added in combination. Isolated KN was ineffective for shoot bud formation. The maximum average number of shoot buds per leaf explant was obtained on medium with BAP, KN and 2iP in combination and on medium supplied of isolated BAP.

  5. Tipos e concentrações de citocinina na multiplicação in vitro de pitangueira Types and concentrations of cytokinin on in vitro multiplication of 'pitangueira'

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    Joseane Almeida de Souza

    2008-10-01

    Full Text Available O trabalho teve como objetivo avaliar o efeito de diferentes tipos e concentrações de citocinina na multiplicação in vitro de pitangueira. Os tratamentos constituíram-se de diferentes concentrações de citocinina adicionada ao meio de cultura (zero; cinco; dez µM e três diferentes citocininas (BAP; 2iP; zeatina. O meio utilizado foi WPM suplementado com 100mg L-1 de mio-inositol, 30g L-1 de sacarose e solidificado com ágar na concentração de seisg L-1. Segmentos caulinares com três ou quatro gemas, sem o ápice, foram incubados em frascos contendo 30mL de meio. Após a inoculação, os explantes foram mantidos em sala de crescimento com 16 horas de fotoperíodo com radiação de 27µmol m-2 s-1 e temperatura de 25±2°C. Aos 45 dias de cultivo, foram avaliados o número médio de folhas, o número médio de brotações e o comprimento das brotações. Os resultados permitiram concluir que, para a multiplicação in vitro de pitangueira, deve-se utilizar a menor concentração de BAP, a qual apresentou resultado satisfatório e tem menor custo.This research aimed to assess the effect of different types (BAP, 2ip, zeatin and concentrations (zero; five; ten µM of cytokinin on in vitro multiplication of 'Pitangueira'. The different concentrations were added to the culture medium. The medium used was WPM enriched with 100mg L-1 of myo-inositol, 30g L-1 of sucrose and solidified with agar at sixg L-1 concentration. Stem segments with 3 or 4 buds, without apical meristem, were incubated into flasks containing 30mL of medium. After inoculation, the explants were kept in growth room under 16 hours photoperiod with 27µmol m-2 s-1 radiation and temperature of 25±2°C. At 45th days of cultivation, the average number of leaves and shoots and the shoot length were measured. The results showed that by using lesser concentration of BAP there was better performance and less costs for in vitro multiplication of 'Pitangueira'.

  6. Efeito do BAP no cultivo in vitro de Ocimum selloi Benth Effect of BAP on in vitro culture of Ocimum selloi Benth

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    L.E.F. Monfort

    2012-01-01

    Full Text Available Visando promover a proliferação de brotações em segmentos apicais e nodais de Ocimum selloi em diferentes concentrações de BAP, plantas jovens de 60 dias serviram de doadoras de segmentos apicais e nodais. Os segmentos foram inoculados em meio MS preparado com a metade da concentração dos sais, e acrescido de 1,5% de sacarose e diferentes concentrações de BAP. O experimento foi conduzido no delineamento fatorial, 3 x 4, sendo 3 as posições dos segmentos de O. selloi (segmento apical, primeiro e segundo segmentos nodais e 4 concentrações de BAP (0 - controle; 2; 4 e 6 mg L-1. Aos 30 dias, foram avaliados o número, comprimento e biomassa fresca e seca de brotos e raízes. Os primeiros e segundos segmentos apresentaram melhores resultados na indução de brotos de O. selloi, 7 e 8 brotos/explante, nas diferentes concentrações de BAP; porém, não houve formação de raízes na presença da citocinina. Nas condições testadas, recomenda-se o uso do primeiro e segundo segmento nodal suplementando o meio de cultivo com BAP para a proliferação in vitro de brotações de O. selloi.The present study was undertaken to develop the proliferation of sprouts in apical and nodal segments of Ocimum selloi with different BAP levels. Young plants aged 60 days were used as donors of nodal and apical segments. The segments were inoculated in MS medium at half the concentration of salts supplemented with 1.5% of sucrose and different BAP levels. The experiment was in 3 x 4 factorial arrangement, 3 positions of O. selloi segments (apical segment; first and second nodal segment and 3 BAP levels (0 - control; 2; 4 and 6 mg L-1. After 30 days, the number, the length, and the fresh and dry biomass of sprouts and roots were evaluated. The first and the second segments showed better results in inducing O. selloi sprouts, 7 and 8 sprouts/explant, at the different BAP levels, but there was not root formation in the presence of the cytokinin. Under the

  7. Citocininas na multiplicação in vitro de hortelã-pimenta (Mentha x Piperita L. Cytokinins on in vitro multiplication of peppermint (Mentha x Piperita L.

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    S. A. Asmar

    2011-01-01

    Full Text Available O hortelã-pimenta é um hibrido triplo, Mentha x piperita, utilizado de forma medicinal no tratamento de náuseas, cólicas gastrointestinais, flatulências, cálculos biliares, icterícia, ansiedade, expectoração e expulsão de vermes intestinais. Na micropropagação, o BAP (6-benzilaminopurina e KIN (cinetina têm sido as fontes de citocininas mais empregadas. O objetivo deste trabalho foi avaliar o efeito de reguladores vegetais na multiplicação in vitro de hortelã-pimenta. Explantes constituídos de segmentos nodais provenientes de plântulas já estabelecidas in vitro com aproximadamente 0,5 cm foram inoculados em meio MS, suplementado com diferentes associações de BAP e KIN, em adição de 30 g L-1 de sacarose. As concentrações de citocininas utilizadas foram 0,0; 1,0 e 2,0 mg L-1 de BAP e 0,0; 0,5 e 1,0 mg L-1 de KIN. O delineamento experimental foi o inteiramente casualizado, com nove tratamentos consistindo de cinco frascos cada um, sendo que cada frasco continha quatro explantes. A utilização de 2,0 mg L-1 de BAP promove a multiplicação in vitro de hortelã-pimenta, porém, diminui o índice de sobrevivência. O uso de citocininas aumenta a massa fresca e seca dos explantes e a ausência destes reguladores propicia o alongamento nesta espécie.Peppermint is used for medical treatments of nausea, gastrointestinal cramps, flatulence, gallstones, jaundice, anxiety, sputum and expulsion of intestinal worms. In micropropagation, BAP (6-benzylaminopurine and KIN (kinetin have been the most widely used sources of cytokinins. The aim of this study was to evaluate the effect of plant growth regulators in multiplication of peppermint. Explants consisting of nodal segments from seedlings already established in vitro with approximately 0,5 cm were inoculated on MS medium supplemented with different combinations of BAP and KIN and 30 g L-1 of sucrose. The concentrations of cytokinins used were 0,0; 1,0 and 2,0 mg L-1 of BAP and 0

  8. Benzilaminopurina (BAP e thidiazuron (TDZ na propagação in vitro de Pfaffia glomerata (Spreng. Pedersen Benzylaminopurine (BAP and thidiazuron (TDZ on in vitro propagation of Pfaffia glomerata (Spreng. Pedersen

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    R. Flores

    2009-01-01

    Full Text Available Pfaffia glomerata (Spreng. Pedersen, conhecida como ginseng brasileiro, é muito utilizada pela medicina popular devido suas propriedades fitoterápicas. Este trabalho teve como objetivo avaliar o efeito de concentrações de BAP e de TDZ na propagação in vitro de dois acessos (BRA e JB-UFSM desta espécie. Segmentos nodais, provenientes de plantas assépticas, foram cultivados em meio Murashige e Skoog (MS suplementado com 0, 1 e 5 μM de BAP ou TDZ. Após 30 dias, as plantas foram transferidas para meio MS não suplementado com citocinina. Observou-se que a organogênese in vitro de P. glomerata é genótipo-dependente. O acesso BRA mostrou um maior potencial para a propagação in vitro em relação ao JB-UFSM. O cultivo dos segmentos nodais do acesso BRA em meio com 1 μM de TDZ, seguido pelo subcultivo dos brotos para meio isento de citocininas mostrou ser um método viável para a propagação in vitro devido à alta taxa de multiplicação e o bom desenvolvimento das plantas. No acesso JB-UFSM, o cultivo dos segmentos nodais em meio não acrescido de citocinina mostrou ser o método mais adequado para a produção de um grande número de plantas com alta qualidade.The plant Pfaffia glomerata (Spreng. Pedersen, known as Brazilian ginseng, is extensively used in folk medicine due to its phytotherapic characteristics. This work aimed to evaluate the effect of BAP and TDZ concentrations on the in vitro propagation of two sources (BRA and JB-UFSM of this species. Nodal segments originated from aseptically grown plants were cultured on Murashige and Skoog (MS medium supplemented with 0, 1 and 5 μM BAP or TDZ. After 30 days, the plants were transferred to MS medium without cytokinin. The in vitro organogenesis of P. glomerata is genotype-dependent. The source BRA had greater potential for in vitro propagation than JB-UFSM. The culture of BRA nodal segments on medium with 1 μM TDZ, followed by subcultivation of sprouts on cytokinin

  9. Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L. Efeito de citocininas sobre o acúmulo de minerais e desenvolvimento de brotações de Annona glabra L. in vitro

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    Lenaldo Muniz de Oliveira

    2010-12-01

    Full Text Available Annona glabra is a tropical species that has significant agronomic potential in terms of furnishing fruits for in natura consumption and for the production of phyto-pharmaceuticals. In vitro cultivation has been considered the most promising form of propagation for this species, although large scale utilization of this technique is currently limited by high rates of leaf abscission, reduced rates of explant multiplication and slow bud growth. The present work evaluated the effects of different cytokinins on mineral accumulation in shoots of A. glabra cultivated in vitro, and their effects on growth and survival of these plants. Buds of A. glabra were cultivated in Wood Plant Medium (WPM in the presence of 6-benzilaminopurine (BAP, thidiazuron (TDZ, kinetin (KIN, and zeatin (ZEA. KIN and BAP use resulted in the greatest growth, largest accumulation of dry mass and leaf area development, as well as the greatest survival rate during in vitro cultivation of this species. All cytokinins tested stimulated large accumulations of nitrogen and boron in shoots, but diminished levels of calcium as compared to controls.Annona glabra é uma espécie frutífera tropical que apresenta elevado potencial agronômico pelo fornecimento de frutos para o consumo in natura e pela produção de fitofármacos. O cultivo in vitro tem sido preconizado como a forma mais adequada de propagação para essa espécie, embora sua utilização em larga escala ainda seja limitada pela elevada taxa de abscisão foliar, reduzida taxa de multiplicação dos explantes e crescimento lento das brotações. Objetivou-se, neste trabalho, avaliar o efeito de citocininas sobre o acúmulo de minerais nas brotações de A. glabra cultivadas in vitro e seus reflexos sobre o crescimento e sobrevivência das plantas nesse tipo de ambiente. Brotações de A. glabra foram cultivadas em meio Wood Plant Medium (WPM, na presença de 6-benzilaminopurina (BAP, thidiazuron (TDZ, cinetina (KIN e

  10. Influência de citocininas na micropropagação de Mentha x gracilis Sole Cytokinin influence on Mentha x gracilis Sole micropropagation

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    T.M.B Garlet

    2011-01-01

    Full Text Available Mentha x gracilis Sole é um híbrido que produz óleos essenciais ricos em monoterpenos. Tendo em vista a propagação clonal desta planta, segmentos nodais provenientes de plantas assépticas, foram cultivados em meio de Murashige e Skoog (MS suplementado com 0; 0,5; 1,0 e 2,0 µM de cinetina, benzilaminopurina (BAP ou thidiazuron (TDZ. Após 30 dias, as plantas foram transferidas para meio MS não suplementado com citocinina. Os melhores resultados foram obtidos em meio suplementado com 2 µM de TDZ, mostrando ser método viável para a produção rápida de grande número de mudas. Após a transferência das plantas para a casa de vegetação, as plantas propagadas com TDZ apresentam maior número de tricomas glandulares.Mentha x gracilis Sole is a hybrid that produces essential oils rich in monoterpenes. Aimed at the clonal propagation of this plant, nodal segments from aseptic plants were cultured in Murashige and Skoog (MSO medium supplemented with 0; 0.5; 1.0 and 2.0 µM kinetin, benzyl adenine (BAP or thidiazuron (TDZ. After 30 days, plants were transferred to MOS medium without cytokinin supplementation. The best results were obtained in medium supplemented with 2 µM TDZ, which proved to be a viable method for the rapid production of a large number of seedlings. After transference to the greenhouse, plants propagated with TDZ had a larger number of glandular trichomes.

  11. Efeitos de citocininas sobre a anatomia foliar e o crescimento de Annona glabra L. durante o cultivo in vitro e ex vitro Effects of cytokinins on the leaf anatomy and growth of Annona glabra L. during in vitro and ex vitro culture

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    Lenaldo Muniz de Oliveira

    2008-08-01

    Full Text Available O efeito de diferentes fontes de citocininas durante o cultivo in vitro de A. glabra sobre características anatômicas de folhas e crescimento das plantas foi avaliado neste trabalho. BAP (6-benzilaminopurina e KIN (cinetina induziram aumento na espessura do mesofilo, enquanto que ZEA (zeatina promoveu aumento na densidade e no índice estomático e no desenvolvimento do sistema vascular de folhas. A utilização de KIN e BAP proporcionou maior desenvolvimento e taxa de sobrevivência das plantas durante as fases de enraizamento e aclimatização.The effect of different sources of cytokinins during the in vitro cultivation of A. glabra on anatomical characteristics of leaves and plant growth was evaluated in this work. BAP (6-benzilaminopurine and KIN (kinetin induced an increase in leaf mesophyll thickness, while the ZEA (zeatin promoted an increase in density and stomatic index and development of leaves vascular system. The utilization of KIN and BAP improved higher plant development and survival rate during the acclimatization and rooting phases.

  12. Concentrações de BAP sobre a proliferação in vitro de brotos de Lippia alba [(Mill.N.E.Brown] BAP concentrations on in vitro proliferation of Lippia alba [(Mill. N.E.Brown] shoots

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    S.A. Asmar

    2012-01-01

    Full Text Available Lippia alba é uma planta de origem brasileira, pertencente à família Verbenaceae. É conhecida por combater a insônia e a asma, além de possuir ação anticonvulsivante e antifúngica. A micropropagação tem sido utilizada para multiplicação de várias espécies com propriedades medicinais, e o BAP é a citocinina sintética mais utilizada para a multiplicação in vitro. O objetivo deste trabalho foi estabelecer a concentração adequada de BAP na multiplicação in vitro de L. alba. Explantes constituídos de segmentos nodais provenientes de plântulas já estabelecidas in vitro com aproximadamente 0,5 cm foram inoculados em meio MS, suplementado com 0,0; 0,5; 1,0 e 1,5 mg L-1 de BAP em adição de 30 g L-1 de sacarose. A utilização de 1,5 mg L-1 de BAP promove a multiplicação in vitro de L. alba. A ausência deste regulador propicia maior número de folhas e o uso na concentração de 0,5 mg L-1 aumentou as massas fresca e seca de parte aérea nesta espécie.Lippia alba is a Brazilian plant, belonging to the Verbenaceae family. It is known to combat insomnia and asthma, also has antifungal and anticonvulsant actions. Micropropagation has been used for propagation of various species with medicinal properties, and BAP is the most widely used synthetic cytokinin for in vitro multiplication. The aim of this study was to determine the appropriate concentration of BAP on in vitro multiplication of L. alba. Explants consisting of nodal segments from seedlings already established in vitro with approximately 0.5 cm were inoculated on MS medium supplemented with 0; 0.5; 1.0 and 1.5 mg L-1 of BAP and 30 g L-1 of sucrose. The use of 1.5 mg L-1 of BAP promotes the in vitro multiplication of L. alba. The absence of this regulator provides a greater number of leaves and its use at the concentration of 0.5 mg L-1 increased shoots' fresh and dry weights in this specie.

  13. Comparison of several methods for BAP measurement.

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    Nakajima, J; Murata, Y; Sakamoto, M

    2006-01-01

    It has been more important for management of water quality to estimate the amount of bioavailable phosphorus (BAP) in suspended solids (SS) entering lakes and estuaries. AGP test or extraction by 0.1 mol l(-1) NaOH (C-BOD) is widely used. Recently, highly bioavailable phosphorus (HBAP) was introduced to indicate a more easily soluble and bioavailable fraction using successive extraction by 0.1 mol l(-1) HCl and 0.1 mol l(-1) NaOH. New biologically measured BAP (B-BAP) using bacterial respiration activity was introduced in this paper. B-BAP was estimated from oxygen uptake rate (OUR), which was measured by a respiratory meter for BOD measurement using a pressure sensor. B-BAP is useful for a rapid and direct measurement of phosphorus bioavailability. B-BAP, HBAP and C-BAP in river SS were measured and compared with each other. The percentages of HBAP and B-BAP to PP were large in the urban river, while the percentage of NaOH-P or C-BAP was large in the rivers flowing in agricultural areas. By comparison with phosphorus fractions in paddy soil and activated sludge it was suggested that SS in the rivers flowing in agricultural areas mainly consisted of clay, silt or sand, while the SS in the urban river consisted of a large percentage of organic particles as well. Phosphorus in SS was suggested to be more easily bioavailable in the urban river than the rivers in agricultural areas. The ratio of C-BAP/B-BAP was large in the rivers in agricultural areas and small in the urban river. As HBAP contents were almost similar to B-BAP contents in the river SS, HBAP can be a suitable index of phosphorus indicating easily and rapidly the bioavailable fraction in SS.

  14. Gibberellin and cytokinin effects on soybean growth Giberelina e citocinina no crescimento da soja

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    Vagner Maximino Leite

    2003-01-01

    Full Text Available Soybean is an important crop in Brazil. Nonetheless, there are no reports on the use of plant growth regulator potential in relation to this crop in the national literature. To better understand the role of these compounds, a pot experiment was carried out to study effects of GA3 and cytokinin on the vegetative growth of the soybean. GA3 (50 mg L-1 was applied as seed treatment, leaving plants with water application as control. GA3 (100 mg L-1 and cytokinin (30 mg L-1 were sprayed on leaves at the physiological stage V3/V4, and 15 days after, cytokinin (30 mg L-1, also as foliar spray. Seed treatment decreased plant emergence and initial soybean root growth, but as the season progressed, differences in root growth disappeared; plants were shorter, and presented a decrease in the number of nodes, in stem diameter, in leaf area and in dry matter yield. Conversely, foliar application of GA3 led to an increase in plant height, first node height and stem diameter. Leaf area and dry matter production also increased as a result of GA3 foliar application. There was no effect of exogenous gibberellin and cytokinin on the number of soybean leaves, number of stem branches and root dry matter. Joint application of gibberellin and cytokinin tended to inhibit gibberellin effects. Cytokinin applied to leaves during soybean vegetative growth was not effective in modifying any of the evaluated plant growth variables.A soja é uma das principais culturas no Brasil, porém não há relatos do potencial de utilização dos fitorreguladores nessa cultura na literatura nacional. Para saber mais sobre estes compostos, um experimento em vasos foi conduzido para estudar o efeito do GA3 e citocinina (CK sobre o crescimento vegetativo e floração da soja. GA3 (50 mg L-1 foi aplicado como tratamento de sementes. Plantas somente com aplicação de água foram aplicadas como controle. Foram feitas duas aplicações foliares, sendo na primeira aplicado 100 mg L-1 de GA3

  15. Influência do meio de cultura, tipo e concentração de citocininas na multiplicação in vitro de amoreira-preta e framboeseira The influence of culture medium, cytokinin type and concentrations on in vitro multiplication of blackberry and raspberry

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    Luciene Nolasco Leitzke

    2010-04-01

    Full Text Available A amoreira-preta e a framboeseira são espécies pouco cultivadas no Brasil, porém representam uma ótima opção de diversificação para pequenas propriedades, por serem rústicas e com elevada produtividade. A propagação dessas espécies dá-se, principalmente, por meio de estacas de raiz e mesmo de hastes novas, porém, já é crescente o interesse pelo uso da micropropagação como um método alternativo de propagação vegetativa de plantas frutíferas. No entanto, existe a necessidade de se ajustar, para cada espécie, e/ou cultivar, as melhores condições de cultivo, para que se obtenha sucesso no processo. Assim, neste trabalho objetivou-se determinar o melhor meio de cultura (MS e WPM, o efeito das citocininas (BAP, 2iP, Zeatina e suas concentrações (0; 7,5 ;15 e 22,5 μM na multiplicação in vitro de amoreira-preta 'Xavante' e de framboeseira 'Batum' e 'Heritage'. De acordo com os resultados obtidos, conclui-se que, o meio MS e a citocinina BAP na concentração de 15 μM foi mais eficiente para ambas as espécies e cultivares, induzindo maior número médio de folhas, brotações e gemas. Quanto ao comprimento das brotações, observou-se um comportamento diferenciado entre espécie, cultivar e regulador. Para amoreira-preta, o cultivo em meio MS na ausência de regulador promoveu o aumento do comprimento das brotações, enquanto que para framboeseira 'Batum' e 'Heritage', melhores resultados foram obtidos na presença de citocininas, respectivamente, Zeatina e 2iP, independentemente das concentrações utilizadas.The blackberry and raspberry are species little cultivated in Brazil. However, they represent a valuable diversification option for small farms, once they are rustic and very productive. Root cuttings and softwood stem are the most widely practiced type of propagation. Recently, there has been an increased interest in the use of micropropagation as an alternative technique for fruit plant propagation. Nevertheless

  16. MULTIPLICAÇÃO IN VITRO DE PORTA-ENXERTOS DO GÊNERO PRUNUS SOB DIFERENTES CONCENTRAÇÕES DE BAP EM DOIS MEIOS DE CULTURA IN VITRO MULTIPLICATION OF PRUNUS ROOTSTOCKS IN DIFFERENT BAP CONCENTRATIONS IN TWO CULTURE MEDIA

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    CARLOS AUGUSTO POSSER SILVEIRA

    2001-12-01

    Full Text Available Este trabalho teve como objetivo determinar a melhor concentração de BAP (6-benzilaminopurina e meio de cultura para a multiplicação in vitro de porta-enxertos de Prunus sp., recentemente introduzidos no Brasil. Os porta-enxertos G x N22, GF 677, Mr.S 2/5, Marianna e Mirabolano foram testados em dois meios de cultura, meio MS e meio MS ¾ (reduzido em 25% dos sais do meio inteiro, combinados com quatro concentrações da citocinina BAP: 0,1; 0,3; 0,5 e 0,7 mg.L-1. Observou-se que os genótipos apresentaram comportamentos diferentes entre si em relação às concentrações de BAP e aos meios. Concluiu-se que, para os porta-enxertos G x N22 e Mr.S 2/5, o melhor meio de multiplicação é o MS ¾ com BAP na concentração de 0,7 mg.L-1; para o porta-enxerto Marianna, o meio MS com BAP na concentração de 0,7 mg.L-1; para o porta-enxerto Mirabolano, o meio MS ¾ com BAP na concentração de 0,5 mg.L-1, sendo que, para este porta-enxerto, o BAP exerce efeito negativo sobre o tamanho das brotações, independentemente do tipo de meio. Já para o porta-enxerto G x N22, este efeito se fez notar apenas no meio MS. O porta-enxerto GF 677 não apresentou bons resultados na propagação in vitro.The purpose of this work was to determine the best BAP concentration and culture media for the in vitro multiplication of Prunus rootstocks starting from nodal segments. The rootstocks G x N22, GF 677, Mr.S 2/5, Marianna and Mirabolano were tested in two culture media (MS and MS ¾ combined with four BAP concentrations (0.1; 0.3; 0.5 and 0.7 mg.L-1. It was observed that the genotypes had different performance as the two media and BAP concentrations are concerned. BAP provided better results in the MS ¾ for the rootstocks G x N22 and Mr.S 2/5. For 'Marianna', the best results were found in the MS at 0.7 mg.L-1 BAP. 'Mirabolano', showed better results in the MS ¾ at 0.5 mg.L-1 BAP and in the two tested media, the shoot length decreased with the increase for

  17. Taxa de multiplicação e efeito residual de diferentes fontes de citocinina no cultivo in vitro de Hancornia speciosa Gomes Proliferation rate and residual effect of different sources of cytokinin on in vitro culture of Hancornia speciosa Gomes

    Directory of Open Access Journals (Sweden)

    Fernanda Pereira Soares

    2011-02-01

    Full Text Available A mangabeira destaca-se por possuir um grande potencial como planta frutífera. Suas sementes apresentam recalcitrância, dificultando sua propagação, o que torna evidente a necessidade da obtenção de mudas por via assexuada. Neste contexto, a cultura de tecidos apresenta-se como uma alternativa a ser utilizada. Entre os fatores que afetam a morfogênese in vitro, as citocininas merecem destaque, pois influenciam na diferenciação de gemas e no crescimento das brotações. Assim sendo, avaliou-se, neste trabalho, a influência de três diferentes fontes de citocinina (6-benzilaminopurina BAP, cinetina CIN e thidiazuron TDZ na indução de brotações in vitro de mangabeira, bem como o efeito residual dessa classe de reguladores no enraizamento e na multiplicação em subcultivos sucessivos. Os resultados mostraram que, entre as citocininas testadas, a 6-benzilaminopurina foi a que induziu maior número de brotações (1,98, gemas (19,22 e folhas (18,86 por explante, tendo promovido a formação de brotos de maior comprimento (4,55 cm. As brotações oriundas de meio WPM basal apresentaram maior facilidade de enraizamento. A 6-benzilaminopurina foi responsável pela maior taxa de multiplicação (9,61 de brotações de mangabeira. Os subcultivos sucessivos diminuíram a capacidade de multiplicação de explantes caulinares da espécie.The mangabeira stands out for having a strong potential for fruit production. Its seeds present recalcitrance, making its propagation difficult, which makes much clear the need to obtain seedlings through asexual methods. In this context, the tissue culture presents as an alternative to be used. Among the factors that affect in vitro morphogenesis, the cytokinins should be highlighted as it influences the differentiation of buds and shoot growth. In this context the influence of three different sources of cytokinins (6-benzylaminopurine BAP, kinetin CIN and thidiazuron TDZ was evaluated on the induction of in

  18. BAP1 has a survival role in cutaneous melanoma.

    Science.gov (United States)

    Kumar, Raj; Taylor, Michael; Miao, Benchun; Ji, Zhenyu; Njauw, Jenny C-N; Jönsson, Göran; Frederick, Dennie T; Tsao, Hensin

    2015-04-01

    Although the pattern of BAP1 inactivation in ocular melanoma specimens and in the BAP1 cutaneous melanoma (CM)/ocular melanoma predisposition syndrome suggests a tumor suppressor function, the specific role of this gene in the pathogenesis of CM is not fully understood. We thus set out to characterize BAP1 in CM and discovered an unexpected pro-survival effect of this protein. Tissue and cell lines analysis showed that BAP1 expression was maintained, rather than lost, in primary melanomas compared with nevi and normal skin. Genetic depletion of BAP1 in melanoma cells reduced proliferation and colony-forming capability, induced apoptosis, and inhibited melanoma tumor growth in vivo. On the molecular level, suppression of BAP1 led to a concomitant drop in the protein levels of survivin, a member of anti-apoptotic proteins and a known mediator of melanoma survival. Restoration of survivin in melanoma cells partially rescued the growth-retarding effects of BAP1 loss. In contrast to melanoma cells, stable overexpression of BAP1 into immortalized but non-transformed melanocytes did suppress proliferation and reduce survivin. Taken together, these studies demonstrate that BAP1 may have a growth-sustaining role in melanoma cells, but that its impact on ubiquitination underpins a complex physiology, which is context and cell dependent. PMID:25521456

  19. BAP e substratos na aclimatização de plântulas de gloxínia (Sinningia speciosa Lood. Hiern. provenientes de cultura de tecidos BAP and substrates on gloxinia (Sinningia speciosa Lood. Hiern. plantlets from tissue culture acclimatization

    Directory of Open Access Journals (Sweden)

    Adriano Bortolotti da Silva

    2003-04-01

    Full Text Available A gloxínia é uma planta ornamental cultivada pela exoticidade e variação de coloração de suas flores. Objetivou-se avaliar a influência residual da citocinina 6-Benzilaminopurina (BAP usada durante a cultura in vitro sobre o processo de aclimatização de gloxínia (Sinningia speciosa Lood. Hiern.. As concentrações de 0,0; 0,5; 1 e 2,0 mg L-1 de BAP, em cultivo in vitro, foram combinadas com os substratos: vermiculita, plantmax® e vermiculita + plantmax®, durante o processo de aclimatização. Após o processo de inoculação in vitro, o material foi transferido para sala de crescimento com temperatura de 26 ± 1ºC, intensidade luminosa de 35 m mol m² s-1 e fotoperíodo de 16 horas, permanecendo nessas condições por 60 dias. Após esse período, as plantas obtidas foram plantadas nos diferentes substratos. As avaliações foram efetuadas após 120 dias, em casa-de-vegetação, registrando-se o número de brotos, peso da matéria fresca do sistema radicular, peso da matéria seca da planta e número de flores. Os melhores resultados foram obtidos com o cultivo em substrato plantmax® ou plantmax�� + vermiculita advinda de meio de cultura in vitro isento de BAP.The present work aimed to evaluate the influence of concentrations of 6-benzylaminopurine (BAP used in acclimatization of gloxinia (Sinningia speciosa Lood. Hiern. Concentrations of BAP (0.0; 0.5; 1.0 and 2.0 mg L-1 in vitro and substrates (vermiculite, plantmax®, and vermiculite+plantmax® were tested for acclimatization in every possible combination. After in vitro inoculation, the material was transferred to a growth chamber with temperature of 26±1ºC and light intensity of 35 m mol m-2 s-1 for 16 hours, remaining under these conditions for 60 days. After that period, the plants obtained were transferred to pots containing substrates in a greenhouse. The evaluations were performed 120 days after cultivation. It were measured number of shoots, roots dry weight, above

  20. Loss of BAP1 Expression Occurs Frequently in Intrahepatic Cholangiocarcinoma

    Science.gov (United States)

    Andrici, Juliana; Goeppert, Benjamin; Sioson, Loretta; Clarkson, Adele; Renner, Marcus; Stenzinger, Albrecht; Tayao, Michael; Watson, Nicole; Farzin, Mahtab; Toon, Christopher W.; Smith, Ross C.; Mittal, Anubhav; Samra, Jaswinder S.; Hugh, Thomas J.; Chou, Angela; Lawlor, Rita T.; Weichert, Wilko; Schirmacher, Peter; Sperandio, Nicola; Ruzzenente, Andrea; Scarpa, Aldo; Gill, Anthony J.

    2016-01-01

    Abstract BRCA1-associated protein 1 (BAP1) is a deubiquitinating enzyme that functions as a tumor suppressor gene. Double hit BAP1 inactivation has been reported in a range of tumor types, including intrahepatic cholangiocarcinoma (ICC), sometimes in association with germline mutation. We performed immunohistochemistry for BAP1 on a well-characterized cohort of 211 ICC patients undergoing surgical resection with curative intent at 3 institutions based in 3 different countries. The median age at diagnosis was 65 years (range, 36.5–86) and 108 (51%) were men. Negative staining for BAP1 (defined as completely absent nuclear staining in the presence of positive internal controls in nonneoplastic cells) occurred in 55 ICCs (26%). BAP1 loss predicted a strong trend toward improved median survival of 40.80 months (95% CI, 28.14–53.46) versus 24.87 months (95% CI, 18.73–31.01), P = 0.059). In a multivariate model including age, sex, BAP1 status, tumor stage, tumor grade, lymphovascular invasion, and tumor size, female sex was associated with improved survival (hazard ratio [HR] 0.54; 95% CI, 0.34–0.85), while advanced tumor stage and lymphovascular invasion (HR 1.89; 95% CI, 1.09–3.28) correlated with decreased survival. In a multivariate analysis, high grade tumors were associated with BAP1 loss (odds ratio [OR] 3.32; 95% CI, 1.29–8.55), while lymphatic invasion was inversely associated with BAP1 loss (OR 0.36; 95% CI, 0.13–0.99). In conclusion, we observed a trend toward improved prognosis in ICC associated with absent expression of BAP1 and an association of BAP1 loss with higher histological grade and absent lymphatic invasion. Female sex was associated with improved survival while advanced tumor stage and lymphatic invasion were associated with decreased survival. PMID:26765459

  1. Calcium inhibits bap-dependent multicellular behavior in Staphylococcus aureus.

    Science.gov (United States)

    Arrizubieta, María Jesús; Toledo-Arana, Alejandro; Amorena, Beatriz; Penadés, José R; Lasa, Iñigo

    2004-11-01

    Bap (biofilm-associated protein) is a 254-kDa staphylococcal surface protein implicated in formation of biofilms by staphylococci isolated from chronic mastitis infections. The presence of potential EF-hand motifs in the amino acid sequence of Bap prompted us to investigate the effect of calcium on the multicellular behavior of Bap-expressing staphylococci. We found that addition of millimolar amounts of calcium to the growth media inhibited intercellular adhesion of and biofilm formation by Bap-positive strain V329. Addition of manganese, but not addition of magnesium, also inhibited biofilm formation, whereas bacterial aggregation in liquid media was greatly enhanced by metal-chelating agents. In contrast, calcium or chelating agents had virtually no effect on the aggregation of Bap-deficient strain M556. The biofilm elicited by insertion of bap into the chromosome of a biofilm-negative strain exhibited a similar dependence on the calcium concentration, indicating that the observed calcium inhibition was an inherent property of the Bap-mediated biofilms. Site-directed mutagenesis of two of the putative EF-hand domains resulted in a mutant strain that was capable of forming a biofilm but whose biofilm was not inhibited by calcium. Our results indicate that Bap binds Ca2+ with low affinity and that Ca2+ binding renders the protein noncompetent for biofilm formation and for intercellular adhesion. The fact that calcium inhibition of Bap-mediated multicellular behavior takes place in vitro at concentrations similar to those found in milk serum supports the possibility that this inhibition is relevant to the pathogenesis and/or epidemiology of the bacteria in the mastitis process.

  2. A recurrent germline BAP1 mutation and extension of the BAP1 tumor predisposition spectrum to include basal cell carcinoma

    DEFF Research Database (Denmark)

    Wadt, Karin Anna Wallentin; Aoude, L G; Johansson, P;

    2015-01-01

    We report four previously undescribed families with germline BRCA1-associated protein-1 gene (BAP1) mutations and expand the clinical phenotype of this tumor syndrome. The tumor spectrum in these families is predominantly uveal malignant melanoma (UMM), cutaneous malignant melanoma (CMM) and meso......We report four previously undescribed families with germline BRCA1-associated protein-1 gene (BAP1) mutations and expand the clinical phenotype of this tumor syndrome. The tumor spectrum in these families is predominantly uveal malignant melanoma (UMM), cutaneous malignant melanoma (CMM......) and mesothelioma, as previously reported for germline BAP1 mutations. However, mutation carriers from three new families, and one previously reported family, developed basal cell carcinoma (BCC), thus suggesting inclusion of BCC in the phenotypic spectrum of the BAP1 tumor syndrome. This notion is supported...... by the finding of loss of BAP1 protein expression by immunochemistry in two BCCs from individuals with germline BAP1 mutations and no loss of BAP1 staining in 53 of sporadic BCCs consistent with somatic mutations and loss of heterozygosity of the gene in the BCCs occurring in mutation carriers. Lastly, we...

  3. From the Eyes of the Front Line: BCBAs Evaluate BAP

    OpenAIRE

    Dixon, Mark R; Reed, Derek; Smith, Tristam

    2013-01-01

    Master's-level Board Certified Behavior Analysts® were emailed an anonymous webbased survey regarding the journal Behavior Analysis in Practice (BAP). Following a 96-hour response window, 284 completed surveys were obtained. Data revealed that many participants regard the journal as beneficial to their profession, yet considered it too expensive and in need of a sharper focus on practitioner issues. Most supported BAP's emphasis on empirical research, and many recommended additional content a...

  4. PORTA-ENXERTOS, CITOCININAS, RETARDANTES DE CRESCIMENTO E URACIL NA FERTILIDADE DE GEMAS DE VIDEIRAS APIRÊNICAS

    Directory of Open Access Journals (Sweden)

    PLÍNIO SALGADO FONSECA DE MELO

    2012-01-01

    Full Text Available The growth retardants Paclobutrazol (PBZ and Cycocel (CCC had been used, in spraying, associates or not with two cytokinins: Benziladenina (BAP and Thidiazuron (TDZ, and a nitrogenous base, the Uracil, with the purpose of studying its effect in the bud fertility of the 'Superior Seedless', grafted on the 'Harmony' and IAC-766 'Campinas', and of the 'Crimson Seedless' and 'Thompson Seedless', grafted on the 'Harmony', in the conditions of the São Francisco Valley, Brazil. It was observed that the separately use of the products had not promoted significant differences in the studied of the bud fertility, but, the association of the growth retardants with the TDZ it induce the 'Thompson Seedless' to a bigger productivity, and that the expression of the bud fertility of the 'Seedless Superior' benefits on to being grafted on the 'Harmony' rootstock.

  5. The bioartificial pancreas (BAP): Biological, chemical and engineering challenges.

    Science.gov (United States)

    Iacovacci, Veronica; Ricotti, Leonardo; Menciassi, Arianna; Dario, Paolo

    2016-01-15

    The bioartificial pancreas (BAP) represents a viable solution for the treatment of type 1 diabetes (T1D). By encapsulating pancreatic cells in a semipermeable membrane to allow nutrient, insulin and glucose exchange, the side effects produced by islets and whole organ transplantation-related immunosuppressive therapy can be circumvented. Several factors, mainly related to materials properties, capsule morphology and biological environment, play a key role in optimizing BAP systems. The BAP is an extremely complex delivery system for insulin. Despite considerable efforts, in some instances meeting with limited degree of success, a BAP capable of restoring physiological pancreas functions without the need for immunosuppressive drugs and of controlling blood glucose levels especially in large animal models and a few clinical trials, does not exist. The state of the art in terms of materials, fabrication techniques and cell sources, as well as the current status of commercial devices and clinical trials, are described in this overview from an interdisciplinary viewpoint. In addition, challenges to the creation of effective BAP systems are highlighted including future perspectives in terms of component integration from both a biological and an engineering viewpoint.

  6. Bap: a family of surface proteins involved in biofilm formation.

    Science.gov (United States)

    Lasa, Iñigo; Penadés, José R

    2006-03-01

    A group of surface proteins sharing several structural and functional features is emerging as an important element in the biofilm formation process of diverse bacterial species. The first member of this group of proteins was identified in a Staphylococcus aureus mastitis isolate and was named Bap (biofilm-associated protein). As common structural features, Bap-related proteins: (i) are present on the bacterial surface; (ii) show a high molecular weight; (iii) contain a core domain of tandem repeats; (iv) confer upon bacteria the capacity to form a biofilm; (v) play a relevant role in bacterial infectious processes; and (vi) can occasionally be contained in mobile elements. This review summarizes recent studies that have identified and assigned roles to Bap-related proteins in biofilm biology and virulence.

  7. From the Eyes of the Front Line: BCBAs Evaluate BAP.

    Science.gov (United States)

    Dixon, Mark R; Reed, Derek; Smith, Tristam

    2013-01-01

    Master's-level Board Certified Behavior Analysts(®) were emailed an anonymous webbased survey regarding the journal Behavior Analysis in Practice (BAP). Following a 96-hour response window, 284 completed surveys were obtained. Data revealed that many participants regard the journal as beneficial to their profession, yet considered it too expensive and in need of a sharper focus on practitioner issues. Most supported BAP's emphasis on empirical research, and many recommended additional content areas such as clinical case formulations, objective product information, and a layout that blends the features of an empirical journal and a popular magazine. In summary, this survey indicates that, as a practitioner-oriented journal, BAP has the potential to occupy a valuable niche for master's-level behavior analysts and that journal leaders might enhance its value by modifying its marketing, content, and structure.

  8. From the Eyes of the Front Line: BCBAs Evaluate BAP.

    Science.gov (United States)

    Dixon, Mark R; Reed, Derek; Smith, Tristam

    2013-01-01

    Master's-level Board Certified Behavior Analysts(®) were emailed an anonymous webbased survey regarding the journal Behavior Analysis in Practice (BAP). Following a 96-hour response window, 284 completed surveys were obtained. Data revealed that many participants regard the journal as beneficial to their profession, yet considered it too expensive and in need of a sharper focus on practitioner issues. Most supported BAP's emphasis on empirical research, and many recommended additional content areas such as clinical case formulations, objective product information, and a layout that blends the features of an empirical journal and a popular magazine. In summary, this survey indicates that, as a practitioner-oriented journal, BAP has the potential to occupy a valuable niche for master's-level behavior analysts and that journal leaders might enhance its value by modifying its marketing, content, and structure. PMID:25729504

  9. Quality in Anthurium andraeanum after postharvest BAP treatment

    DEFF Research Database (Denmark)

    Favero, B.T.; Lima, G.P.P.; Cavasini, R.;

    quality and sugar levels. Two experiments were done, one where inflorescences were sprayed 0, 100 and 200 mg L-1 of BAP and kept under 23±1°C and relative humidity of 65±5% for evaluations, and in the second experiment BAP concentrations were reduced to 0, 50 and 100 mg L-1 and inflorescences were cold....... In the first experiment FW loss was significantly lower from the 14th until 26th day after harvest. Anthurium sprayed with 100 and 200 mg L-1 showed significantly less fresh weight loss when compared to control stems and in the second experiment 50 and 100 mg L-1 did not reduce fresh weight loss. Means...... of scores from quality analysis, blueing and loss of gloss of spathes and spadix necrosis, were significantly lower in BAP sprayed anthurium (100 and 200 mg L-1) than in control flowers and by lowering BAP spray concentration and cold storing stems for two days this positive effect was only observed...

  10. CALOGÊNESE DE TECIDO FOLIAR DE PORTA-ENXERTO DE MACIEIRA M.7 (MALUS sp. INDUZIDA POR BAP E CPPU

    Directory of Open Access Journals (Sweden)

    MARTINS CARLOS ROBERTO

    2001-01-01

    Full Text Available O trabalho foi conduzido com o objetivo de estudar a organogênese de macieira (Malus sp, após a obtenção de calo por meio de explantes de folhas do porta-enxerto M.7 multiplicado in vitro. O experimento foi conduzido no Laboratório de Cultura de Tecidos da Embrapa Clima Temperado, utilizando folhas com a superfície abaxial e adaxial em contato com o meio, com ou sem escarificação; associados às citocininas Benzilaminopurina (BAP e Forchlorfenuron (CPPU na concentração de 5mM. Utilizou-se o meio básico MS acrescido de sacarose (30 g.L-1 , mio-inositol (100 mg.L-1 e ágar (6 g.L-1, além do regulador de crescimento Ácido Naftalenoácetico (ANA 0,5mM. Os tratamentos permaneceram por três semanas no escuro sob temperatura ambiente, o que propiciou 100% de formação de calos, sendo em seguida submetidos a fotoperíodo de 16 horas com intensidade luminosa de 20 mE.m-2.s-1 e temperatura de 25 ± 2ºC. Os explantes escarificados proporcionaram maior intensidade de calo do que a utilização de explantes intatos. Explantes escarificados com a superfície abaxial em contato com o meio proporcionaram maior intensidade de calo, independentemente de o meio conter BAP ou CPPU. O uso da escarificação, associado ao CPPU, promoveu uma maior intensidade de calo, independentemente da superfície do disco foliar. A superior regeneração de calos foi alcançada em condição de superfície abaxial do disco foliar associado ao CPPU. Portanto, o uso de explantes escarificados com a superfície abaxial em contato com o meio proporcionou aumento da intensidade de calo. O uso do explante escarificado em meio contendo CPPU proporcionou maior intensidade de calo, independentemente da superfície do disco foliar em contato com o meio.

  11. An Emerging Model for BAP1’s Role in Regulating Cell Cycle Progression

    OpenAIRE

    Eletr, Ziad M.; Wilkinson, Keith D.

    2011-01-01

    BRCA1-associated protein-1 (BAP1) is a 729 residue, nuclear-localized deubiquitinating enzyme (DUB) that displays tumor suppressor properties in the BAP1-null NCI-H226 lung carcinoma cell line. Studies that have altered BAP1 cellular levels or enzymatic activity have reported defects in cell cycle progression, notably at the G1/S transition. Recently BAP1 was shown to associate with the transcriptional regulator host cell factor 1 (HCF-1). The BAP1/HCF-1 interaction is mediated by the HCF-1 K...

  12. EFEITOS DE GIBERELINAS, CITOCININAS E DO NITRATO DE POTÁSSIO, NO PROCESSO GERMINATIVO DE SEMENTES DE LIMOEIRO `CRAVO' (Citrus limonia OSBECK

    Directory of Open Access Journals (Sweden)

    Leonel Sarita

    1999-01-01

    Full Text Available Foram estudados os efeitos de reguladores vegetais do grupo das giberelinas e citocininas, bem como do nitrato de potássio na germinação de sementes do limoeiro `Cravo' (Citrus limonia Osbeck. O experimento foi realizado, contendo papel de filtro como substrato para a germinação das sementes, regulado à temperatura de 25oC. As sementes foram retiradas de frutos maduros no final da safra do limoeiro `Cravo', lavadas, secas à sombra e armazenadas durante 11 dias em câmara fria. Em seguida, receberam tratamento com os fitorreguladores e KNO3 por 24 horas, de acordo com os tratamentos: KNO3 0,1% e 0,2%; GA3 50, 100 e 250 mg.L-1; GA4 + GA7 + fenilmetilaminopurina 100 mg.L-1; fenilmetilaminopurina 20 mg.L-1 e água destilada (testemunha. As avaliações foram iniciadas 15 dias após a semeadura, em intervalos de 5 dias. Conclui-se que os reguladores vegetais utilizados não afetaram o processo germinativo das sementes e que os tratamentos com nitrato de potássio 0,1% e 0,2% exerceram efeito inibitório sobre a germinação.

  13. Dispersal of Bap-mediated Staphylococcus aureus biofilm by proteinase K.

    Science.gov (United States)

    Kumar Shukla, Sudhir; Rao, Toleti Subba

    2013-02-01

    The dominant role of biofilm-associated protein (Bap) in Staphylococcus aureus biofilm development prompted us to investigate Bap as a potential target for proteinase-mediated biofilm dispersion. Biofilm assay in microtitre plates showed that proteinase K hampered the early adhesion of cells as well as biofilm development. Proteinase K treatment of 24- and 48-h-old biofilms showed enhanced dispersion of bap-positive S. aureus biofilm; however, proteinase K did not affect the bap-negative S. aureus biofilm. When antibiotics were used in combination with proteinase K, significant enhancement in antibiotic action was noticed against bap-positive S. aureus biofilm. This study establishes that antibiotics in combination with proteinase K can be used for controlling S. aureus biofilms in whose development Bap surface protein has a major role. We propose that Bap protein could be a potential target for therapeutic control of S. aureus infections (for example, bovine mastitis).

  14. Protection against Acinetobacter baumannii infection via its functional deprivation of biofilm associated protein (Bap).

    Science.gov (United States)

    Fattahian, Yaser; Rasooli, Iraj; Mousavi Gargari, Seyed Latif; Rahbar, Mohammad Reza; Darvish Alipour Astaneh, Shakiba; Amani, Jafar

    2011-12-01

    Acinetobacter baumannii, a major nosocomial pathogen, has remarkable capacity to acquire antimicrobial resistance attributable to its biofilm formation ability. Biofilm associated protein (Bap), a specific cell surface protein, is directly involved in biofilm formation by A. baumannii and plays a major role in bacterial infectious processes. In the present study we cloned, expressed and purified a 371 amino acid subunit of Bap. Mice were immunized using recombinant Bap subunit. They were then challenged with A. baumannii to evaluate the immunogenicity and protectivity of Bap subunit. Humoral immune response to Bap was determined by ELISA. Injection of Bap subunit resulted in high antibody titers. Decrease in bacterial cell counts of the immunized mice was evident 18 h after challenge. Reaction of antibodies against Bap with several strains suggests that not only immunodominant regions of Bap in A. baumannii strains are conserved but also have the same epitope presenting pattern in different strains. Immunodominant region of Bap possesses target sites for a protective humoral immune response to A. baumannii. This seems to be a conserved region erecting efficacy of Bap as an appropriate vaccine candidate.

  15. A novel BAP1 mutation is associated with melanocytic neoplasms and thyroid cancer.

    Science.gov (United States)

    McDonnell, Kevin J; Gallanis, Gregory T; Heller, Kathleen A; Melas, Marilena; Idos, Gregory E; Culver, Julie O; Martin, Sue-Ellen; Peng, David H; Gruber, Stephen B

    2016-03-01

    Germline mutations in the tumor suppressor gene, BRCA-1 associated protein (BAP1), underlie a tumor predisposition syndrome characterized by increased risk for numerous cancers including uveal melanoma, melanocytic tumors and mesothelioma, among others. In the present study we report the identification of a novel germline BAP1 mutation, c.1777C>T, which produces a truncated BAP1 protein product and segregates with cancer. Family members with this mutation demonstrated a primary clinical phenotype of autosomal dominant, early-onset melanocytic neoplasms with immunohistochemistry (IHC) of these tumors demonstrating lack of BAP1 protein expression. In addition, family members harboring the BAP1 c.1777C>T germline mutation developed other neoplastic disease including thyroid cancer. IHC analysis of the thyroid cancer, as well, demonstrated loss of BAP1 protein expression. Our investigation identifies a new BAP1 mutation, further highlights the relevance of BAP1 as a clinically important tumor suppressor gene, and broadens the range of cancers associated with BAP1 inactivation. Further study will be required to understand the full scope of BAP1-associated neoplastic disease.

  16. EFEITO DE CITOCININA E AUXINAS SOBRE O ESTABELECIMEMTO IN VITRO DE Gerbera jamesonii Bolus ex Hook cv. Appelbloesem A PARTIR DE CAPÍTULOS JOVENS

    Directory of Open Access Journals (Sweden)

    Márcio Henrique Pereira Barbosa

    1993-12-01

    Full Text Available Na obtenção de plântulas in vitro da cv. Appelbloesem, foram utilizados capítulos jovens como explantes iniciais sendo culturados em meio MS (MURASHIGE & SKOOG, 1962 solidificado com 0,7% de ágar e suplementado com adenina (80mg/l, tirosina (100mg/l e diferentes concentrações e combinações de BAP (6-benzilaminopurina com AIA (ácido indole-3-acético e AIA com 2,4-D (ácido 2,4-diclorofenoxiacético. A avaliação foi feita levando-se em consideração o número de brotos adventícios regenerados por explante, formação de calos e desenvolvimento dos capítulos estabelecidos in vitro. Conseguiu-se melhores resultados com os níveis de 3, 6 e 9mg/l de BAP, sendo que para o nível de 3mg/l de BAP regenerou-se em média, duas brotações por explante, para o nível de 6mg/l de BAP, três brotações e para o nível de 9mg/l, uma brotação. O uso do 2,4-D induz maior formação de calos para a fase de estabelecimento da cultura.

  17. A BAP1 Mutation in a Danish Family Predisposes to Uveal Melanoma and Other Cancers

    DEFF Research Database (Denmark)

    Aoude, Lauren G; Wadt, Karin; Bojesen, Anders;

    2013-01-01

    Truncating germline mutations in the tumor suppressor gene BRCA-1 associated protein-1 (BAP1) have been reported in families predisposed to developing a wide range of different cancer types including uveal melanoma and cutaneous melanoma. There has also been an association between amelanotic tumor...... development and germline BAP1 mutation suggesting a possible phenotypic characteristic of BAP1 mutation carriers. Though there have been many types of cancer associated with germline BAP1 mutation, the full spectrum of disease association is yet to be ascertained. Here we describe a Danish family...... with predominantly uveal melanoma but also a range of other tumor types including lung, neuroendocrine, stomach, and breast cancer; as well as pigmented skin lesions. Whole-exome sequencing identified a BAP1 splice mutation located at c.581-2A>G, which leads to a premature truncation of BAP1 in an individual...

  18. BAP1 facilitates diagnostic objectivity, classification, and prognostication in malignant pleural mesothelioma.

    Science.gov (United States)

    McGregor, Stephanie M; Dunning, Ryan; Hyjek, Elizabeth; Vigneswaran, Wickii; Husain, Aliya N; Krausz, Thomas

    2015-11-01

    BRCA-associated protein 1 (BAP1) has emerged as a promising biomarker for malignant pleural mesothelioma (MPM). Loss of BAP1 expression can occur by a variety of mechanisms, but reports on incidence are variable and the clinical significance is unclear. In order to investigate the diagnostic and prognostic significance of BAP1, we constructed a tissue microarray consisting of 111 MPM cases and performed BAP1 immunohistochemistry. BAP1 was lost in 77% of epithelioid cases (n=58) but was retained in all sarcomatoid cases (n=10); 49% of biphasic cases showed loss (n=43), and BAP1-negative cases demonstrated loss of staining in both the epithelioid and sarcomatoid components. All non-neoplastic mesothelial tissues (n=20) retained BAP1, resulting in a sensitivity, specificity, positive predictive value, and negative predictive value of 61%, 100%, 100%, and 32%, respectively. Moreover, BAP1 expression in spindled mesothelium enabled discrimination of reactive and malignant cells, thus providing a more objective means of distinguishing epithelioid from biphasic morphology compared to histology alone. Nonetheless, BAP1 staining was patchy in some benign mesothelial neoplasms, which raises concern for using BAP1 in small biopsies. Kaplan-Meier analysis demonstrated a significant improvement in overall survival with BAP1 loss, but this did not reach significance in multivariate analysis accounting for histologic subtype. When only epithelioid cases were analyzed there was a trend toward increased survival, but it did not reach significance. We conclude that BAP1 loss is frequent in epithelioid MPM, which is in turn associated with improved survival, and that it can have additional clinical significance by facilitating histologic classification.

  19. Analytical validation of serum bone alkaline phosphatase (BAP OSTASE) on Liaison

    OpenAIRE

    Cavalier, Etienne; Rozet, Eric; Carlisi, Agnès; Bekaert, Anne-Catherine; ROUSSELLE, Olivier; Hubert, Philippe; Chapelle, Jean-Paul; Delanaye, Pierre

    2009-01-01

    Background: The goal of this study was to validate the DiaSorin Liaison BAP OSTASE, a new method for measurement of bone alkaline phosphatase (BAP), and to compare this method with the Beckman-Coulter Access Ostase. We also wanted to establish the reference range for BAP in adults and children. Methods: We determined the precision, functional sensitivity, recovery, linearity and measurement uncertainty, accuracy profile and β-expectation limits. We defined an adult reference interval using i...

  20. Bap-dependent biofilm formation by pathogenic species of Staphylococcus: evidence of horizontal gene transfer?

    Science.gov (United States)

    Tormo, M Angeles; Knecht, Erwin; Götz, Friedrich; Lasa, Iñigo; Penadés, José R

    2005-07-01

    The biofilm-associated protein (Bap) is a surface protein implicated in biofilm formation by Staphylococcus aureus isolated from chronic mastitis infections. The bap gene is carried in a putative composite transposon inserted in SaPIbov2, a mobile staphylococcal pathogenicity island. In this study, bap orthologue genes from several staphylococcal species, including Staphylococcus epidermidis, Staphylococcus chromogenes, Staphylococcus xylosus, Staphylococcus simulans and Staphylococcus hyicus, were identified, cloned and sequenced. Sequence analysis comparison of the bap gene from these species revealed a very high sequence similarity, suggesting the horizontal gene transfer of SaPIbov2 amongst them. However, sequence analyses of the flanking region revealed that the bap gene of these species was not contained in the SaPIbov2 pathogenicity island. Although they did not contain the icaADBC operon, all the coagulase-negative staphylococcal isolates harbouring bap were strong biofilm producers. Disruption of the bap gene in S. epidermidis abolished its capacity to form a biofilm, whereas heterologous complementation of a biofilm-negative strain of S. aureus with the Bap protein from S. epidermidis bestowed the capacity to form a biofilm on a polystyrene surface. Altogether, these results demonstrate that Bap orthologues from coagulase-negative staphylococci induce an alternative mechanism of biofilm formation that is independent of the PIA/PNAG exopolysaccharide.

  1. SarA positively controls bap-dependent biofilm formation in Staphylococcus aureus.

    Science.gov (United States)

    Trotonda, María Pilar; Manna, Adhar C; Cheung, Ambrose L; Lasa, Iñigo; Penadés, José R

    2005-08-01

    The biofilm-associated protein Bap is a staphylococcal surface protein involved in biofilm formation. We investigated the influence of the global regulatory locus sarA on bap expression and Bap-dependent biofilm formation in three unrelated Staphylococcus aureus strains. The results showed that Bap-dependent biofilm formation was diminished in the sarA mutants by an agr-independent mechanism. Complementation studies using a sarA clone confirmed that the defect in biofilm formation was due to the sarA mutation. As expected, the diminished capacity to form biofilms in the sarA mutants correlated with the decreased presence of Bap in the bacterial surface. Using transcriptional fusion and Northern analysis data, we demonstrated that the sarA gene product acts as an activator of bap expression. Finally, the bap promoter was characterized and the transcriptional start point was mapped by the rapid amplification of cDNA ends technique. As expected, we showed that purified SarA protein binds specifically to the bap promoter, as determined by gel shift and DNase I footprinting assays. Based on the previous studies of others as well as our work demonstrating the role for SarA in icaADBC and bap expression, we propose that SarA is an essential regulator controlling biofilm formation in S. aureus.

  2. Phase-variable expression of the biofilm-associated protein (Bap) in Staphylococcus aureus.

    Science.gov (United States)

    Tormo, M Angeles; Ubeda, Carles; Martí, Miguel; Maiques, Elisa; Cucarella, Carme; Valle, Jaione; Foster, Timothy J; Lasa, Iñigo; Penadés, José R

    2007-06-01

    A process of phase variation is described that affects the expression of Bap (biofilm-associated protein) in Staphylococcus aureus. Upon subculture of the Bap-positive S. aureus strain V329 on Congo red agar, spontaneous smooth biofilm-negative colonies appeared at a low frequency (5 x 10(-4)). Northern blot analysis of these variants with a bap-specific gene probe showed that transcription of the bap gene did not occur. However, DNA typing, Southern blot hybridization and DNA sequencing did not show any differences between the parent V329 strain and the biofilm-negative variants. The biofilm-negative phenotype reverted to wild-type at a similar frequency upon subculture of Bap-negative variants in liquid media. Experimental infection of ovine mammary glands with Bap-negative variants showed that phase variation occurred in vivo, because Bap-expressing, biofilm-positive revertants were isolated from infected mammary glands. The absence of Bap correlated with increased adherence to fibrinogen and fibronectin. It is possible that S. aureus can detach from a biofilm by switching to a Bap-negative state.

  3. An emerging model for BAP1's role in regulating cell cycle progression.

    Science.gov (United States)

    Eletr, Ziad M; Wilkinson, Keith D

    2011-06-01

    BRCA1-associated protein-1 (BAP1) is a 729 residue, nuclear-localized deubiquitinating enzyme (DUB) that displays tumor suppressor properties in the BAP1-null NCI-H226 lung carcinoma cell line. Studies that have altered BAP1 cellular levels or enzymatic activity have reported defects in cell cycle progression, notably at the G1/S transition. Recently BAP1 was shown to associate with the transcriptional regulator host cell factor 1 (HCF-1). The BAP1/HCF-1 interaction is mediated by the HCF-1 Kelch domain and an HCF-1 binding motif (HBM) within BAP1. HCF-1 is modified with ubiquitin in vivo, and ectopic studies suggest BAP1 deubiquitinates HCF-1. HCF-1 is a chromatin-associated protein thought to both activate and repress transcription by linking appropriate histone-modifying enzymes to a subset of transcription factors. One known role of HCF-1 is to promote cell cycle progression at the G1/S boundary by recruiting H3K4 histone methyltransferases to the E2F1 transcription factor so that genes required for S-phase can be transcribed. Given the robust associations between BAP1/HCF-1 and HCF-1/E2Fs, it is reasonable to speculate that BAP1 influences cell proliferation at G1/S by co-regulating transcription from HCF-1/E2F-governed promoters. PMID:21484256

  4. Isolation of marine benzo[a]pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization

    Institute of Scientific and Technical Information of China (English)

    WU Yirui; HE Tengteng; ZHONG Mingqi; ZHANG Yueling; LI Enmin; HUANG Tongwang; HU Zhong

    2009-01-01

    A bacterial strain BAP5 with a relatively high degradation ability of benzo[a]pyrene (BaP) was isolated from marine sediments of Xiamen Western Sea, China and identified as Ochrobactrum sp. according to 16S rRNA gene sequence as well as biolog microbial identification system. Strain BAP5 could grow in mineral salt medium with 50 mg/L of BaP and degrade about 20% BaP after 30 d of incubation. Ochrobactrum sp. BAP5 was able to utilize other polycyclic aromatic hydrocarbons (PAHs) (such as phenanthrene, pyrene and fluoranthene) as the sole carbon source and energy source, suggesting its potential application in PAHs bioremediation. The profile of total soluble protein from Ochrobactrum sp. BAP5 was also investigated. Some over- and special-expressed proteins of strain BAP5 when incubated with the presence of BaP were detected by using two-dimensional polyacrylamide gel electrophoresis, and found to be related with PAHs metabolism, DNA translation, and energy production based on peptide fingerprint analysis through matrix-assisted laser desorption/ionization-time of flight mass spectrometry.

  5. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein

    Energy Technology Data Exchange (ETDEWEB)

    Li, Yan; Jain, Neeraj; Limpanawat, Suweeraya; To, Janet [School of Biological Sciences, Nanyang Technological University, 637551 (Singapore); Quistgaard, Esben M. [Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm (Sweden); Nordlund, Par [School of Biological Sciences, Nanyang Technological University, 637551 (Singapore); Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm (Sweden); Thanabalu, Thirumaran [School of Biological Sciences, Nanyang Technological University, 637551 (Singapore); Torres, Jaume, E-mail: jtorres@ntu.edu.sg [School of Biological Sciences, Nanyang Technological University, 637551 (Singapore)

    2015-08-15

    The small hydrophobic (SH) protein is a short channel-forming polypeptide encoded by the human respiratory syncytial virus (hRSV). Deletion of SH protein leads to the viral attenuation in mice and primates, and delayed apoptosis in infected cells. We have used a membrane-based yeast two-hybrid system (MbY2H) and a library from human lung cDNA to detect proteins that bind SH protein. This led to the identification of a membrane protein, B-cell associated protein 31 (BAP31). Transfected SH protein co-localizes with transfected BAP31 in cells, and pulls down endogenous BAP31. Titration of purified C-terminal endodomain of BAP31 against isotopically labeled SH protein in detergent micelles suggests direct interaction between the two proteins. Given the key role of BAP31 in protein trafficking and its critical involvement in pro- and anti-apoptotic pathways, this novel interaction may constitute a potential drug target. - Highlights: • A yeast two-hybrid system (MbY2H) detected BAP31 as a binder of RSV SH protein. • Transfected SH and BAP31 co-localize in lung epithelial cells. • Endogenous BAP31 is pulled down by RSV SH protein. • BAP31 endodomain interacts with the N-terminal α-helix of SH protein in micelles. • This interaction is proposed to be a potential drug target.

  6. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein

    International Nuclear Information System (INIS)

    The small hydrophobic (SH) protein is a short channel-forming polypeptide encoded by the human respiratory syncytial virus (hRSV). Deletion of SH protein leads to the viral attenuation in mice and primates, and delayed apoptosis in infected cells. We have used a membrane-based yeast two-hybrid system (MbY2H) and a library from human lung cDNA to detect proteins that bind SH protein. This led to the identification of a membrane protein, B-cell associated protein 31 (BAP31). Transfected SH protein co-localizes with transfected BAP31 in cells, and pulls down endogenous BAP31. Titration of purified C-terminal endodomain of BAP31 against isotopically labeled SH protein in detergent micelles suggests direct interaction between the two proteins. Given the key role of BAP31 in protein trafficking and its critical involvement in pro- and anti-apoptotic pathways, this novel interaction may constitute a potential drug target. - Highlights: • A yeast two-hybrid system (MbY2H) detected BAP31 as a binder of RSV SH protein. • Transfected SH and BAP31 co-localize in lung epithelial cells. • Endogenous BAP31 is pulled down by RSV SH protein. • BAP31 endodomain interacts with the N-terminal α-helix of SH protein in micelles. • This interaction is proposed to be a potential drug target

  7. Loss of BAP1 function leads to EZH2-dependent transformation

    Science.gov (United States)

    LaFave, Lindsay M.; Béguelin, Wendy; Koche, Richard; Teater, Matt; Spitzer, Barbara; Chramiec, Alan; Papalexi, Efthymia; Keller, Matthew D.; Hricik, Todd; Konstantinoff, Katerina; Micol, Jean-Baptiste; Durham, Benjamin; Knutson, Sarah K.; Campbell, John E.; Blum, Gil; Shi, Xinxu; Doud, Emma H.; Krivtsov, Andrei V.; Chung, Young Rock; Khodos, Inna; de Stanchina, Elisa; Ouerfelli, Ouathek; Adusumilli, Prasad S.; Thomas, Paul M.; Kelleher, Neil L.; Luo, Minkui; Keilhack, Heike; Abdel-Wahab, Omar; Melnick, Ari; Armstrong, Scott A.

    2015-01-01

    Introductory Paragraph BAP1 and ASXL1 interact to form a polycomb deubiquitinase complex that removes monoubiquitin from histone H2A lysine 119 (H2AK119Ub). However, BAP1 and ASXL1 are mutated in distinct cancer types, consistent with independent roles in regulating epigenetic state and malignant transformation. Here we demonstrate that Bap1 loss results in increased trimethylated histone H3 lysine 27 (H3K27me3), elevated Ezh2 expression, and enhanced repression of Polycomb Repressive Complex 2 (PRC2) targets. These findings contrast with the reduction in H3K27me3 seen with Asxl1 loss. Conditional deletion of Bap1 and Ezh2 in vivo abrogates the myeloid progenitor expansion induced by Bap1 loss alone. Loss of Bap1 results in a marked decrease in H4K20 monomethylation (H4K20me1). Consistent with a role for H4K20me1 in EZH2 transcriptional regulation, expression of SETD8, the H4K20me1 methyltransferase, reduces EZH2 expression and abrogates the proliferation of BAP1-mutant cells. Further, mesothelioma cells that lack BAP1 are sensitive to EZH2 pharmacologic inhibition, suggesting a novel therapeutic approach for BAP1-mutant malignancies. PMID:26437366

  8. BAP1 cancer syndrome: malignant mesothelioma, uveal and cutaneous melanoma, and MBAITs

    Directory of Open Access Journals (Sweden)

    Carbone Michele

    2012-08-01

    Full Text Available Abstract Background BRCA1–associated protein 1 (BAP1 is a tumor suppressor gene located on chromosome 3p21. Germline BAP1 mutations have been recently associated with an increased risk of malignant mesothelioma, atypical melanocytic tumors and other neoplasms. To answer the question if different germline BAP1 mutations may predispose to a single syndrome with a wide phenotypic range or to distinct syndromes, we investigated the presence of melanocytic tumors in two unrelated families (L and W with germline BAP1 mutations and increased risk of malignant mesothelioma. Methods Suspicious cutaneous lesions were clinically and pathologically characterized and compared to those present in other families carrying BAP1 mutations. We then conducted a meta-analysis of all the studies reporting BAP1-mutated families to survey cancer risk related to the germline BAP1 mutation (means were compared using t-test and proportions were compared with Pearson χ2 test or two-tailed Fisher’s exact test. Results Melanocytic tumors: of the five members of the L family studied, four (80% carried a germline BAP1 mutation (p.Gln684* and also presented one or more atypical melanocytic tumors; of the seven members of W family studied, all carried a germline BAP1 mutation (p.Pro147fs*48 and four of them (57% presented one or more atypical melanocytic tumors, that we propose to call “melanocytic BAP1-mutated atypical intradermal tumors” (MBAITs. Meta-analysis: 118 individuals from seven unrelated families were selected and divided into a BAP1-mutated cohort and a BAP1-non-mutated cohort. Malignant mesothelioma, uveal melanoma, cutaneous melanoma, and MBAITs prevalence was significantly higher in the BAP1-mutated cohort (p ≤ 0.001. Conclusions Germline BAP1 mutations are associated with a novel cancer syndrome characterized by malignant mesothelioma, uveal melanoma, cutaneous melanoma and MBAITs, and possibly by other cancers. MBAITs provide physicians with a

  9. Role of biofilm-associated protein bap in the pathogenesis of bovine Staphylococcus aureus.

    Science.gov (United States)

    Cucarella, Carme; Tormo, M Angeles; Ubeda, Carles; Trotonda, M Pilar; Monzón, Marta; Peris, Critòfol; Amorena, Beatriz; Lasa, Iñigo; Penadés, José R

    2004-04-01

    Staphylococcus aureus is a common cause of intramammary infections, which frequently become chronic, associated with the ability of the bacteria to produce biofilm. Here, we report a relationship between the ability to produce chronic bovine mastitis and biofilm formation. We have classified bovine mastitis S. aureus isolates into three groups based on the presence of particular genetic elements required for biofilm formation: group 1 (ica(+) bap(+)), group 2 (ica(+), bap negative), and group 3 (ica negative, bap negative). Overall, animals naturally infected with group 1 and 2 isolates had a lower milk somatic cell count than those infected with isolates of group 3. In addition, Bap-positive isolates were significantly more able to colonize and persist in the bovine mammary gland in vivo and were less susceptible to antibiotic treatments when forming biofilms in vitro. Analysis of the structural bap gene revealed the existence of alternate forms of expression of the Bap protein in S. aureus isolates obtained under field conditions throughout the animal's life. The presence of anti-Bap antibodies in serum samples taken from animals with confirmed S. aureus infections indicated the production of Bap during infection. Furthermore, disruption of the ica operon in a bap-positive strain had no effect on in vitro biofilm formation, a finding which strongly suggested that Bap could compensate for the deficiency of the PIA/PNAG product (a biofilm matrix polysaccharide). Altogether, these results demonstrate that, in the bovine intramammary gland, the presence of Bap may facilitate a biofilm formation connected with the persistence of S. aureus.

  10. Effect of Commercial Synthappret BAP Treatment on the Tailorabilitv of Light-Weight Worsted Wool Fabrics

    Institute of Scientific and Technical Information of China (English)

    Wang Gehui(王革辉); Zhang Weiyuan(张渭源); Postle, R.; Phillips, D.

    2001-01-01

    By means of measurement with the FAST instruments,the effect of commercial Synthappret BAP treatment on the tailorability of light-weight wool worsted fabrics has been investigated. It was found that the commercial Synthappret BAP treatment improved the tailorability of the light-weight wool fabrics mainly by increasing the bending stiffness of the light-weight wool fabrics.

  11. FilmoBap : Filmoteca de Bande à Part, Escola de Cinema

    OpenAIRE

    Sanz Revert, Susana

    2015-01-01

    FilmoBap és una web per a la gestió de préstec de pel·lícules d'una escola. FilmoBap es una web para la gestión de préstamo de películas de una escuela. Bachelor thesis for the Multimedia program.

  12. EFEITOS DE GIBERELINAS, CITOCININAS E DO NITRATO DE POTÁSSIO NO CRESCIMENTO E DESENVOLVIMENTO DO PORTA-ENXERTO DE LIMOEIRO `CRAVO'

    Directory of Open Access Journals (Sweden)

    S. LEONEL

    1996-05-01

    Full Text Available Os efeitos de reguladores vegetais do grupo das giberelinas e citocininas, bem como do nitrato de potássio, foram estudados no crescimento e desenvolvimento do porta-enxerto de limoeiro `Cravo' (Citrus limonia Osbeck. As sementes foram extraídas de frutos maduros, lavadas, secas à sombra e armazenadas em câmara fria (4 - 5oC durante sessenta dias, até o mês de maio/1993, quando foram semeadas nos canteiros de semeadura. As pulverizações com os fitorreguladores foram realizadas no viveiro e tiveram início com as plantas aos 8 meses de idade, contados a partir da semeadura, sendo efetuadas um total de 4 pulverizações, realizadas a intervalos quinzenais. Os tratamentos corresponderam a: 25, 50 e 75 ppm de GA3; 25, 50 e 75 ppm de GA4+GA7+fenilmetil-aminopurina; 20 ppm de fenilmetilpiranil-aminopurina; 0,2% de KNO3 e testemunha (sem pulverização. Os resultados obtidos permitiram concluir que as pulverizações com os reguladores vegetais e com o nitrato de potássio, realizadas no período de janeiro a março (verão, não tiveram efeito na diminuição do tempo de formação das plantas jovens de limoeiro `Cravo', tendo inclusive, o tratamento com KNO3 0,2%, exercido efeito depressivo no desenvolvimento das mesmas.The effects of gibberellins, cytokinins and potassium nitrate on the development of `Rangpur'lime (Citrus limonia Osbeck rootstocks were studied. Seeds from ripe fruits were washed, dried and stored at 4 - 5o C for 60 days. They were planted in nursery plots in May/1993 and when the seedlings had 10 - 20 cm height, they were transplanted to a second nursery stage. The seedlings were sprayed at 15-day intervals, with plant regulators and potassium nitrate, after reaching the age of 8 months. From January to March/1993, the seedlings were sprayed with: 25, 50 and 75 ppm of GA3; 25, 50 and 75 ppm of GA4+7 + phenylmethyl-aminepurine; 20 ppm of phenylmethylpyranil-aminepurine; 0.2% of KNO3. The seedlings planted in May/1993 did not

  13. Different prognostic roles of tumor suppressor gene BAP1 in cancer: A systematic review with meta-analysis.

    Science.gov (United States)

    Luchini, Claudio; Veronese, Nicola; Yachida, Shinichi; Cheng, Liang; Nottegar, Alessia; Stubbs, Brendon; Solmi, Marco; Capelli, Paola; Pea, Antonio; Barbareschi, Mattia; Fassan, Matteo; Wood, Laura D; Scarpa, Aldo

    2016-10-01

    Biallelic inactivation of the tumor suppressor gene BRCA1-associated protein 1 (BAP1) has been demonstrated in several cancers, but its prognostic role has not been completely explained. We aimed to investigate the risk associated with loss of BAP1 (BAP1-) for all-cause mortality, cancer-specific mortality and recurrence of disease in subjects with cancer. PubMed and SCOPUS were searched from database inception until 09/15/2015 without language restrictions. Prospective studies reporting data on prognostic parameters in subjects with cancer, comparing participants with presence of BAP1 (BAP1+) vs. BAP1- were included. Data were summarized using risk ratios (RR) for number of deaths/recurrences and hazard ratios (HR) for time-dependent risk related to BAP1- adjusted for potential confounders. From 261 hits, 12 studies (including 13 cohorts) with 3,447 participants (BAP1-: n = 697; BAP1+: n = 2,750), with a median follow-up over 60 months, were meta-analyzed. Compared to BAP1+, BAP1- significantly increased all-cause mortality, cancer-specific mortality and risk of recurrence in all the tumor types analyzed, except for mesothelioma, in which the presence of BAP1 mutations correlates with a better prognosis. Furthermore, we demonstrated that BAP1 mutated colorectal and renal carcinomas are associated with high-tumor grading (P < 0.0001), and that BAP1 mutated is more common in women than in men (P < 0.0001). In conclusion, on the basis of our meta-analysis, we have demonstrated a peculiar role of BAP1 in influencing the prognosis in cancer. Thus, BAP1 could be considered as an important potential target for personalized medicine. © 2016 Wiley Periodicals, Inc. PMID:27223342

  14. BAP1 suppresses lung cancer progression and is inhibited by miR-31

    Science.gov (United States)

    Wang, Xueliang; Liao, Zhicong; Zhou, Yong; Liu, Yanqing; Wang, Yanbo; Hong, Yeting; Zhou, Xinyan; Yan, Xin; Yu, Min; Ma, Miao; Zhang, Weijie; Guo, Baoliang; Zhang, Jianguo; Zen, Ke; Zhang, Chen-Yu; Wang, Tao; Zhang, Qipeng; Chen, Xi

    2016-01-01

    BRCA1-associated protein-1 (BAP1) is an important nuclear-localized deubiquitinating enzyme that serves as a tumor suppressor in lung cancer; however, its function and its regulation are largely unknown. In this study, we found that BAP1 protein levels were dramatically diminished in lung cancer tissues while its mRNA levels did not differ significantly, suggesting that a post-transcriptional mechanism was involved in BAP1 regulation. Because microRNAs (miRNAs) are powerful post-transcriptional regulators of gene expression, we used bioinformatic analyses to search for miRNAs that could potentially bind BAP1. We predicted and experimentally validated miR-31 as a direct regulator of BAP1. Moreover, we showed that miR-31 promoted proliferation and suppressed apoptosis in lung cancer cells and accelerated the development of tumor growth in xenograft mice by inhibiting BAP1. Taken together, this study highlights an important role for miR-31 in the suppression of BAP1 in lung cancer cells and may provide insights into the molecular mechanisms of lung carcinogenesis. PMID:26885612

  15. A BAP1 mutation in a Danish family predisposes to uveal melanoma and other cancers.

    Directory of Open Access Journals (Sweden)

    Lauren G Aoude

    Full Text Available Truncating germline mutations in the tumor suppressor gene BRCA-1 associated protein-1 (BAP1 have been reported in families predisposed to developing a wide range of different cancer types including uveal melanoma and cutaneous melanoma. There has also been an association between amelanotic tumor development and germline BAP1 mutation suggesting a possible phenotypic characteristic of BAP1 mutation carriers. Though there have been many types of cancer associated with germline BAP1 mutation, the full spectrum of disease association is yet to be ascertained. Here we describe a Danish family with predominantly uveal melanoma but also a range of other tumor types including lung, neuroendocrine, stomach, and breast cancer; as well as pigmented skin lesions. Whole-exome sequencing identified a BAP1 splice mutation located at c.581-2A>G, which leads to a premature truncation of BAP1 in an individual with uveal melanoma. This mutation was carried by several other family members with melanoma or various cancers. The finding expands on the growing profile of BAP1 as an important uveal and cutaneous melanoma tumor suppressor gene and implicates its involvement in the development of lung, and stomach cancer.

  16. Identification of an amyloidogenic peptide from the Bap protein of Staphylococcus epidermidis.

    Science.gov (United States)

    Lembré, Pierre; Vendrely, Charlotte; Martino, Patrick Di

    2014-01-01

    Biofilm associated proteins (Bap) are involved in the biofilm formation process of several bacterial species. The sequence STVTVT is present in Bap proteins expressed by many Staphylococcus species, Acinetobacter baumanii and Salmonella enterica. The peptide STVTVTF derived from the C-repeat of the Bap protein from Staphylococcus epidermidis was selected through the AGGRESCAN, PASTA, and TANGO software prediction of protein aggregation and formation of amyloid fibers. We characterized the self-assembly properties of the peptide STVTVTF by different methods: in the presence of the peptide, we observed an increase in the fluorescence intensity of Thioflavin T; many intermolecular β-sheets and fibers were spontaneously formed in peptide preparations as observed by infrared spectroscopy and atomic force microscopy analyses. In conclusion, a 7 amino acids peptide derived from the C-repeat of the Bap protein was sufficient for the spontaneous formation of amyloid fibers. The possible involvement of this amyloidogenic sequence in protein-protein interactions is discussed.

  17. Identification of an amyloidogenic peptide from the Bap protein of Staphylococcus epidermidis.

    Science.gov (United States)

    Lembré, Pierre; Vendrely, Charlotte; Martino, Patrick Di

    2014-01-01

    Biofilm associated proteins (Bap) are involved in the biofilm formation process of several bacterial species. The sequence STVTVT is present in Bap proteins expressed by many Staphylococcus species, Acinetobacter baumanii and Salmonella enterica. The peptide STVTVTF derived from the C-repeat of the Bap protein from Staphylococcus epidermidis was selected through the AGGRESCAN, PASTA, and TANGO software prediction of protein aggregation and formation of amyloid fibers. We characterized the self-assembly properties of the peptide STVTVTF by different methods: in the presence of the peptide, we observed an increase in the fluorescence intensity of Thioflavin T; many intermolecular β-sheets and fibers were spontaneously formed in peptide preparations as observed by infrared spectroscopy and atomic force microscopy analyses. In conclusion, a 7 amino acids peptide derived from the C-repeat of the Bap protein was sufficient for the spontaneous formation of amyloid fibers. The possible involvement of this amyloidogenic sequence in protein-protein interactions is discussed. PMID:24354773

  18. 26. V avatakse kunstiakadeemia galeriis näitus "BAP vs. CAPO"

    Index Scriptorium Estoniae

    2003-01-01

    Näitusel alapealkirjaga "Kujutav kunst versus kujuteldamatu" näeb maale, graafikat, fotosid, propagandaplakateid, taustaks rahustav muusika (BAP). Osaleb grafitikunstnike rühmitus CAPO, plakatimeister KORK, minimalist Samot Mint

  19. [Effects of BaP exposure on ultrastructures of hepatic cells of Boleophthalmus pectinirostris].

    Science.gov (United States)

    Feng, Tao; Zheng, Weiyun; Ouyang, Gaoliang; Hong, Wanshu

    2003-10-01

    The changes of ultrastructures of hepatic cells of Boleophthalmus pectinirostris were investigated after the fish were exposed under benzo(a) pyrene in different concentrations under experimental condition. The results showed that the organelles in hepatic cells of B. pectinirostris were damaged to different extents after the fish was exposed under lower concentration of BaP (0.5 mg.L-1) for up to 7 d, in which, mitochondria and endoplasmic reticulum were the chief organelles affected by BaP exposure. While the fish was exposed under higher concentration of BaP (5 mg.L-1) for 2 h, almost all of the organelles including mitochondria and endoplasmic reticulum in hepatic cells of B. pectinirostr were affected by BaP exposure. The structures of liver cells were seriously damaged. It was demonstrated that BaP could produce multiorganalle lesions in hepatic cells of B. pectinirostris, and the severity extent of such lesions was dependent on the concentration level of BaP.

  20. Staphylococcal Bap Proteins Build Amyloid Scaffold Biofilm Matrices in Response to Environmental Signals.

    Science.gov (United States)

    Taglialegna, Agustina; Navarro, Susanna; Ventura, Salvador; Garnett, James A; Matthews, Steve; Penades, José R; Lasa, Iñigo; Valle, Jaione

    2016-06-01

    Biofilms are communities of bacteria that grow encased in an extracellular matrix that often contains proteins. The spatial organization and the molecular interactions between matrix scaffold proteins remain in most cases largely unknown. Here, we report that Bap protein of Staphylococcus aureus self-assembles into functional amyloid aggregates to build the biofilm matrix in response to environmental conditions. Specifically, Bap is processed and fragments containing at least the N-terminus of the protein become aggregation-prone and self-assemble into amyloid-like structures under acidic pHs and low concentrations of calcium. The molten globule-like state of Bap fragments is stabilized upon binding of the cation, hindering its self-assembly into amyloid fibers. These findings define a dual function for Bap, first as a sensor and then as a scaffold protein to promote biofilm development under specific environmental conditions. Since the pH-driven multicellular behavior mediated by Bap occurs in coagulase-negative staphylococci and many other bacteria exploit Bap-like proteins to build a biofilm matrix, the mechanism of amyloid-like aggregation described here may be widespread among pathogenic bacteria.

  1. Staphylococcal Bap Proteins Build Amyloid Scaffold Biofilm Matrices in Response to Environmental Signals

    Science.gov (United States)

    Taglialegna, Agustina; Navarro, Susanna; Ventura, Salvador; Garnett, James A.; Matthews, Steve; Penades, José R.; Lasa, Iñigo; Valle, Jaione

    2016-01-01

    Biofilms are communities of bacteria that grow encased in an extracellular matrix that often contains proteins. The spatial organization and the molecular interactions between matrix scaffold proteins remain in most cases largely unknown. Here, we report that Bap protein of Staphylococcus aureus self-assembles into functional amyloid aggregates to build the biofilm matrix in response to environmental conditions. Specifically, Bap is processed and fragments containing at least the N-terminus of the protein become aggregation-prone and self-assemble into amyloid-like structures under acidic pHs and low concentrations of calcium. The molten globule-like state of Bap fragments is stabilized upon binding of the cation, hindering its self-assembly into amyloid fibers. These findings define a dual function for Bap, first as a sensor and then as a scaffold protein to promote biofilm development under specific environmental conditions. Since the pH-driven multicellular behavior mediated by Bap occurs in coagulase-negative staphylococci and many other bacteria exploit Bap-like proteins to build a biofilm matrix, the mechanism of amyloid-like aggregation described here may be widespread among pathogenic bacteria. PMID:27327765

  2. BAP, 2,4-D e ácido acetilsalicílico na indução e diferenciação de calos em anteras de Coffea arabica L BAP, 2,4-D and acetyl-salicylic acid on the callus induction and differentiation in Coffea arabica L. anthers

    Directory of Open Access Journals (Sweden)

    Adelaide Siqueira Silva

    2009-10-01

    Full Text Available O melhoramento genético do cafeeiro por meio de métodos convencionais é um processo demorado para se obter uma nova cultivar. A redução desse tempo é possível através da produção de linhagens homozigóticas, oriundas de dihaplóides obtidas através da cultura de anteras. Objetivou-se aplicar a técnica da cultura de anteras em diferentes cvs. de Coffea arabica L. para induzir a formação de calos e regenerar plântulas di-haplóides, com uso de reguladores vegetais. Os experimentos foram conduzidos no laboratório de Biotecnologia Vegetal da Universidade Federal de Uberlândia (UFU. Anteras das cultivares Mundo Novo LCP-379-19 e Catuaí Vermelho H2077-2-5-44 foram inoculadas em meio MS suplementado com 2,0 mg L-1 de 2,4-D e AAS, nas concentrações de 0; 8; 16; 32 e 64 mg L-1. Calos de 'Catuaí Vermelho 44' foram subcultivados em meio MS acrescido de diferentes concentrações de BAP (0; 2; 4 e 8 mg L-1 e 2,4-D (0; 1; 2 e 4 mg L-1. Tanto para as cvs. Mundo Novo quanto para Catuaí Vermelho 44 o aumento das concentrações de AAS diminuiu a formação de próembrióides nos calos e somente o 2,4-D foi capaz de promover a formação de calos friáveis, porém o equilíbrio da auxina e da citocinina utilizadas no trabalho, favoreceram a produção de calos friáveis.Coffee plant breeding through conventional methods demands a long time to obtain new cultivars. The reduction of this period is possible through the production of homozygous lines, from dihaploids obtained via anther culture. The aim of this study was to apply the anther culture technique on different C. arabica L. cultivars to induce calli formation and to regenerate dihaploid seedlings with the use of plant growth regulators. The experiments were accomplished in the Plant Biotechnology laboratory at Uberlândia Federal University (UFU. Anthers of the cultivars Mundo Novo LCP-379-19 and Catuaí Vermelho H2077-2-5-44 were inoculated on MS medium supplemented with 2.0 mg L-1

  3. Jumping the gun: Smoking constituent BaP causes premature primordial follicle activation and impairs oocyte fusibility through oxidative stress

    Energy Technology Data Exchange (ETDEWEB)

    Sobinoff, A.P.; Pye, V. [Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW2308 (Australia); Nixon, B.; Roman, S.D. [Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW2308 (Australia); ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Callaghan, NSW2308 (Australia); McLaughlin, E.A., E-mail: eileen.mclaughlin@newcastle.edu.au [Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW2308 (Australia); ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Callaghan, NSW2308 (Australia)

    2012-04-01

    Benzo(a)pyrene (BaP) is an ovotoxic constituent of cigarette smoke associated with pre-mature ovarian failure and decreased rates of conception in IVF patients. Although the overall effect of BaP on female fertility has been documented, the exact molecular mechanisms behind its ovotoxicity remain elusive. In this study we examined the effects of BaP exposure on the ovarian transcriptome, and observed the effects of in vivo exposure on oocyte dysfunction. Microarray analysis of BaP cultured neonatal ovaries revealed a complex mechanism of ovotoxicity involving a small cohort of genes associated with follicular growth, cell cycle progression, and cell death. Histomorphological and immunohistochemical analysis supported these results, with BaP exposure causing increased primordial follicle activation and developing follicle atresia in vitro and in vivo. Functional analysis of oocytes obtained from adult Swiss mice treated neonatally revealed significantly increased levels of mitochondrial ROS/lipid peroxidation, and severely reduced sperm-egg binding and fusion in both low (1.5 mg/kg/daily) and high (3 mg/kg/daily) dose treatments. Our results reveal a complex mechanism of BaP induced ovotoxicity involving developing follicle atresia and accelerated primordial follicle activation, and suggest short term neonatal BaP exposure causes mitochondrial leakage resulting in reduced oolemma fluidity and impaired fertilisation in adulthood. This study highlights BaP as a key compound which may be partially responsible for the documented effects of cigarette smoke on follicular development and sub-fertility. -- Highlights: ► BaP exposure up-regulates canonical pathways linked with follicular growth/atresia. ► BaP causes primordial follicle activation and developing follicle atresia. ► BaP causes oocyte mitochondrial ROS and lipid peroxidation, impairing fertilisation. ► Short term neonatal BaP exposure compromises adult oocyte quality.

  4. Structural and functional studies of BapC protein of Bordetella pertussis.

    Science.gov (United States)

    Riaz, Muhammad Rizwan; Siddiqi, Abdul Rauf; Bokhari, Habib

    2015-05-01

    Bordetella pertussis, the causative agent of whooping cough, attaches to mucosal surface in upper respiratory tract, where it produces a variety of surface associated and secreted autotransporter molecules among others. In this study we have cloned newly identified member of autotransporter family BapC (B. pertussis autotransporter protein C); expressed it in Escherichia coli and characterized it for its different properties. We have also raised antisera to BapC protein; the antisera were used in immunofluorescence assay to determine the surface association of the protein. Results suggest that BapC in B. pertussis Taberman parent is surface exposed when compared with the respective BapC mutant. The neutralizing effect of anti-BapC serum was also evaluated in the presence of active complement proteins and results suggest that antiserum can potentiate the killing of B. pertussis cells in the presence of added source of complement. Structure of the protein was also studied, both α and β domains of the protein were modeled, β domain exhibits typical transmembrane β-barrel porin topology whereas α domain behaves as a characteristic bacterial autotransporter passenger domain.

  5. Cardiovascular adjustments and perceived exertion during exercise on the BAPS board.

    Science.gov (United States)

    Balogun, J A; Pletcher, E A; Miertschin, D E; Martinez, E D; Hoeberlein, T

    1995-01-01

    The Biomechanical Ankle Platform System (BAPS) is widely used in medical rehabilitation; however, the cardiovascular demand and subjective acceptability of the BAPS rehabilitation exercise are presently not documented. The purpose of this study was to evaluate the pattern and magnitude of the cardiovascular adjustments and rating of perceived exertion (RPE) during an acute bout of exercise on the wobble board. Twenty healthy (mean age 24.2 +/- 2.9 years old) subjects exercised for 10 min each on the BAPS board on two different occasions. One test was performed using the small hemisphere board while the other test was performed with the large hemisphere board. The subjects' heart rate (HR), blood pressure (BP), cutaneous blood flow (BF), skin temperature (ST) at the ankle joint, local and overall RPE responses were monitored at specific time periods. Micro-circulation, skin temperature and heart rate were monitored with the LaserFlo Blood Perfusion Monitor, the Physitherm thermocouple and the Cardiotach recorder, respectively. The subjects' rating of perceived effort during exercise was obtained with a 15-point category scale. The result showed significant perturbation of the cardiovascular system during the BAPS exercise. Similarly, both local and overall RPE increased as the duration of exercise increased. The BF and RPE during the large hemisphere exercise session were significantly higher than the small hemisphere session. The subjects' subjective report following the exercise suggests the need for caution in the use of the BAPS board exercise for frail patients and also patients with lower extremity joint pathologies. PMID:8573705

  6. Current management of bipolar affective disorder: is it reflective of the BAP guidelines?

    Science.gov (United States)

    Farrelly, N; Dibben, C; Hunt, N

    2006-01-01

    In October 2003 the British Association of Psychopharmacology (BAP) published evidence-based guidelines on the management of bipolar disorder. The aim of this study was to assess whether the guidelines could provide the basis for examining clinical decisions and the extent to which practice accords with these guidelines. Case notes of out patients with bipolar disorder were reviewed. Demographic details, and treatment recommendations were determined. The management of affective episodes was evaluated and compared with BAP guidelines. In 84 subjects, 224 affective episodes were identified. Treatment was consistent with BAP guidelines in 72% of episodes. Mania was more likely to be managed in accordance with guidelines than depression or mixed episodes. The use of antidepressant medication was the most likely intervention to deviate from recommendations. Reasons for treatments at odds with the guidelines were identified. Our study demonstrates that clinical practice among a range of psychiatrists broadly reflects the guidelines that have been issued by the British Association of Psychopharmacology (BAP). The BAP guidelines offer a practical and auditable basis for the short- and long-term treatment of bipolar affective disorder.

  7. [Research on the degradation of BaP with potassium ferrate characterized by fluorescence].

    Science.gov (United States)

    Chen, Yu-Zhe; He, Qiang; Yu, Dan-Ni; Li, Si; Tan, Xue-Mei

    2012-07-01

    The degradation of Benzo(a)pyrene (BaP) by potassium ferrate was researched by means of multiple fluorescence spectroscopic methods such as synchronous, time-scan, excitation emission matrix (EEM) and photometry, under the optimal condition. Within the degradation process, the characteristics of the BaP's concentration at different time-intervals, and the kinetics of the degradation of BaP by potassium ferrate were discussed. From the experimental data, both synchronous and EEM spectra's results showed that the concentration of BaP was reduced 90% by potassium ferrate within 20 s after degradation, and the reaction process was very slow after 60 s. The degradation kinetic equation, ln(F0/Ft) = 0.563 2t + 0.171 2, (R2 = 0.994 2), was obtained through a convenient and fast way combining the time-scan fluorescence data and photometry data, and the photometry included the synchronous photometry and emission photometry. According to the kinetic equation, the degradation of BaP by potassium ferrate was in accord with the order of the first order reaction. So this article could provide a very useful conference for the research on the pollutant degradation by potassium ferrate, especially for the degradation process and the degradation mechanisms.

  8. Biophotonics determination of 6-benzylaminopurine (6-BAP) plant growth regulator using OFRR biosensor

    Science.gov (United States)

    Yang, Gilmo; Kang, Sukwon; Lee, Kangjin; Kim, Giyoung; Son, Jaeryong; Mo, Changyeun

    2010-04-01

    The identification of pesticide and 6-benzylaminopurine (6-BAP) plant growth regulator was carried out using a label-free opto-fluidic ring resonator (OFRR) biosensor. The OFRR sensing platform is a recent advancement in opto-fluidic technology that integrates photonic sensing technology with microfluidics. It features quick detection time, small sample volume, accurate quantitative and kinetic results. The most predominant advantage of the OFRR integrated with microfluidics is that we can potentially realize the multi-channel and portable biosensor that detects numerous analytes simultaneously. Antisera for immunoassay were raised in rabbits against the 6-BAP-BSA conjugate. Using the immunization protocol and unknown cytokinin reacting with same antibody, comparable sensitivity and specificity were obtained. 6-BAP antibody was routinely used for cytokinin analysis. A sensitive and simple OFRR method with a good linear relationship was developed for the determination of 6-BAP. The detection limit was also examined. The biosensor demonstrated excellent reproducibility when periodically exposed to 6-BAP.

  9. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein.

    Science.gov (United States)

    Li, Yan; Jain, Neeraj; Limpanawat, Suweeraya; To, Janet; Quistgaard, Esben M; Nordlund, Par; Thanabalu, Thirumaran; Torres, Jaume

    2015-08-01

    The small hydrophobic (SH) protein is a short channel-forming polypeptide encoded by the human respiratory syncytial virus (hRSV). Deletion of SH protein leads to the viral attenuation in mice and primates, and delayed apoptosis in infected cells. We have used a membrane-based yeast two-hybrid system (MbY2H) and a library from human lung cDNA to detect proteins that bind SH protein. This led to the identification of a membrane protein, B-cell associated protein 31 (BAP31). Transfected SH protein co-localizes with transfected BAP31 in cells, and pulls down endogenous BAP31. Titration of purified C-terminal endodomain of BAP31 against isotopically labeled SH protein in detergent micelles suggests direct interaction between the two proteins. Given the key role of BAP31 in protein trafficking and its critical involvement in pro- and anti-apoptotic pathways, this novel interaction may constitute a potential drug target.

  10. Bap, a biofilm matrix protein of Staphylococcus aureus prevents cellular internalization through binding to GP96 host receptor.

    Science.gov (United States)

    Valle, Jaione; Latasa, Cristina; Gil, Carmen; Toledo-Arana, Alejandro; Solano, Cristina; Penadés, José R; Lasa, Iñigo

    2012-01-01

    The biofilm matrix, composed of exopolysaccharides, proteins, nucleic acids and lipids, plays a well-known role as a defence structure, protecting bacteria from the host immune system and antimicrobial therapy. However, little is known about its responsibility in the interaction of biofilm cells with host tissues. Staphylococcus aureus, a leading cause of biofilm-associated chronic infections, is able to develop a biofilm built on a proteinaceous Bap-mediated matrix. Here, we used the Bap protein as a model to investigate the role that components of the biofilm matrix play in the interaction of S. aureus with host cells. The results show that Bap promotes the adhesion but prevents the entry of S. aureus into epithelial cells. A broad analysis of potential interaction partners for Bap using ligand overlayer immunoblotting, immunoprecipitation with purified Bap and pull down with intact bacteria, identified a direct binding between Bap and Gp96/GRP94/Hsp90 protein. The interaction of Bap with Gp96 provokes a significant reduction in the capacity of S. aureus to invade epithelial cells by interfering with the fibronectin binding protein invasion pathway. Consistent with these results, Bap deficient bacteria displayed an enhanced capacity to invade mammary gland epithelial cells in a lactating mice mastitis model. Our observations begin to elucidate the mechanisms by which components of the biofilm matrix can facilitate the colonization of host tissues and the establishment of persistent infections.

  11. Bap, a biofilm matrix protein of Staphylococcus aureus prevents cellular internalization through binding to GP96 host receptor.

    Directory of Open Access Journals (Sweden)

    Jaione Valle

    Full Text Available The biofilm matrix, composed of exopolysaccharides, proteins, nucleic acids and lipids, plays a well-known role as a defence structure, protecting bacteria from the host immune system and antimicrobial therapy. However, little is known about its responsibility in the interaction of biofilm cells with host tissues. Staphylococcus aureus, a leading cause of biofilm-associated chronic infections, is able to develop a biofilm built on a proteinaceous Bap-mediated matrix. Here, we used the Bap protein as a model to investigate the role that components of the biofilm matrix play in the interaction of S. aureus with host cells. The results show that Bap promotes the adhesion but prevents the entry of S. aureus into epithelial cells. A broad analysis of potential interaction partners for Bap using ligand overlayer immunoblotting, immunoprecipitation with purified Bap and pull down with intact bacteria, identified a direct binding between Bap and Gp96/GRP94/Hsp90 protein. The interaction of Bap with Gp96 provokes a significant reduction in the capacity of S. aureus to invade epithelial cells by interfering with the fibronectin binding protein invasion pathway. Consistent with these results, Bap deficient bacteria displayed an enhanced capacity to invade mammary gland epithelial cells in a lactating mice mastitis model. Our observations begin to elucidate the mechanisms by which components of the biofilm matrix can facilitate the colonization of host tissues and the establishment of persistent infections.

  12. Evaluation of the presence of the bap gene in Staphylococcus aureus isolates recovered from human and animals species.

    Science.gov (United States)

    Vautor, E; Abadie, G; Pont, A; Thiery, R

    2008-03-18

    The implication of biofilm in chronic bacterial infection in many species has triggered an increasing interest in the characterization of genes involved in biofilm formation. The bap gene is a newly identified gene that encodes the biofilm-associated protein, BAP, which is involved in biofilm formation in Staphylococcus aureus. So far the bap gene has only been found in a small proportion of S. aureus strains from bovine mastitis in Spain. In order to study the presence of the bap gene in S. aureus isolates obtained from other species and various locations, a collection of 262 isolates was tested by PCR, using published primers and dot-blot. The results indicated that none of the isolates carried the bap gene suggesting that the prevalence of this gene among S. aureus isolates should be very low.

  13. [Study on continued degradation of BaP and its metabolites].

    Science.gov (United States)

    Zang, Shu-yan; Li, Pei-Jun; Zhou, Qi-xing; Wang, Xin; Lin, Gui-feng; Wang, Juan

    2006-12-01

    Two metabolites, cis-BP4, 5-dihydrodiol and cis-BP7, 8-dihydrodiol, were identified by high-performance liquid chromatography (HPLC) during the degradation of BaP by Bacillus-07 (BA-07). The two metabolites were hardly further metabolized for their toxicity to microorganism. To promote degradation of BaP and decrease accumulation of cis-BP4, 5-dihydrodiol and cis-BP7, 8-dihydrodiol, two methods (degradation only by BA-07, degradation by coupling the BA-07 and KMnO4) were compared. In addition, parameters of continued degradation of BaP and the two metabolites were optimized under the experiment conditions. The results showed that (1)the method of coupling the chemical oxidation and biodegradation (BA-07 and KMnO4) was better than only biodegradation (BA-07); (2) residue rate of cis-BP4, 5-dihydrodiol was higher than that of cis-BP7, 8-dihydrodiol when the samples were determined in the same time; (3)the effect of continued degradation was the best when the initial concentration of BaP was 40 microg/mL, pH value of the culture was 7.0, co-metabolic substrates was sodium succinate. Meanwhile, it was put forward that the method of coupling the chemical oxidation and biodegradation was effective on continued degradation of persistent organic contaminants in the environment.

  14. The gene bap, involved in biofilm production, is present in Staphylococcus spp. strains from nosocomial infections.

    Science.gov (United States)

    Potter, Amina; Ceotto, Hilana; Giambiagi-Demarval, Marcia; dos Santos, Kátia Regina Netto; Nes, Ingolf F; Bastos, Maria do Carmo de Freire

    2009-06-01

    This study analyzed ten strains of coagulase-negative staphylococci (CNS) involved in nosocomial infections in three Brazilian hospitals. Their antibiotic susceptibility profile showed that most strains exhibited multiple antibiotic resistance and possessed the mecA gene. The ability of these strains to adhere to polystyrene microtiter plates was also tested and nine of them proved to be biofilm producers at least in one of the three conditions tested: growth in TSB, in TSB supplemented with NaCl, or in TSB supplemented with glucose. The presence of the bap gene, which codes for the biofilm-associated protein (Bap), was investigated in all ten strains by PCR. AU strains were bop-positive and DNA sequencing experiments confirmed that the fragments amplified were indeed part of a bap gene. The presence of the icaA gene, one of the genes involved in polysaccharide intercellular adhesin (PIA) formation, was also detected by PCR in eight of the ten strains tested. The two icaA-negative strains were either weak biofilm producer or no biofilm producer, although they were bop-positive. To our knowledge, this is the first report demonstrating the presence of the bap gene in nosocomial isolates of CNS, being also the first report on the presence of this gene in Staphylococcus haemolyticus and S. cohnii.

  15. A cryptic BAP1 splice mutation in a family with uveal and cutaneous melanoma, and paraganglioma

    DEFF Research Database (Denmark)

    Wadt, K.; Choi, J.; Chung, J.Y.;

    2012-01-01

    as paraganglioma, breast cancer, and suspected mesothelioma cases in the family. Bioinformatic analysis and splicing assays demonstrated that this mutation creates a strong cryptic splice donor, resulting in aberrant splicing and a truncating frameshift of the BAP1 transcript. Somatic loss of the wild-type allele...

  16. CDKN2A and BAP1 germline mutations predispose to melanoma and mesothelioma.

    Science.gov (United States)

    Betti, M; Aspesi, A; Biasi, A; Casalone, E; Ferrante, D; Ogliara, P; Gironi, L C; Giorgione, R; Farinelli, P; Grosso, F; Libener, R; Rosato, S; Turchetti, D; Maffè, A; Casadio, C; Ascoli, V; Dianzani, C; Colombo, E; Piccolini, E; Pavesi, M; Miccoli, S; Mirabelli, D; Bracco, C; Righi, L; Boldorini, R; Papotti, M; Matullo, G; Magnani, C; Pasini, B; Dianzani, I

    2016-08-10

    BAP1 germline mutations predispose to a cancer predisposition syndrome that includes mesothelioma, cutaneous melanoma, uveal melanoma and other cancers. This co-occurrence suggests that these tumors share a common carcinogenic pathway. To evaluate this hypothesis, we studied 40 Italian families with mesothelioma and/or melanoma. The probands were sequenced for BAP1 and for the most common melanoma predisposition genes (i.e. CDKN2A, CDK4, TERT, MITF and POT1) to investigate if these genes may also confer susceptibility to mesothelioma. In two out of six families with both mesothelioma and melanoma we identified either a germline nonsense mutation (c.1153C > T, p.Arg385*) in BAP1 or a recurrent pathogenic germline mutation (c.301G > T, p.Gly101Trp) in CDKN2A. Our study suggests that CDKN2A, in addition to BAP1, could be involved in the melanoma and mesothelioma susceptibility, leading to the rare familial cancer syndromes. It also suggests that these tumors share key steps that drive carcinogenesis and that other genes may be involved in inherited predisposition to malignant mesothelioma and melanoma. PMID:27181379

  17. Removals of non-analogous OTC and BaP in AMCBR with and without primary substrate.

    Science.gov (United States)

    Çelebi, Hakan; Gök, Oğuzhan; Sponza, Delia Teresa

    2016-01-01

    Anaerobic biodegradation of mixed non-analogous two substrates was studied in a binary system with and without the primary substrate using an anaerobic multichamber bed (AMCBR). In the binary mixture, the biodegradation of less-degradable oxytetracycline (OTC) was restarted in the presence of more degradable benzo[a]pyrene (BaP) in the initial runs of the AMCBR, but enhanced biodegradation of the more recalcitrant OTC occurs in the later runs of the AMCBR due to enhanced biomass growth on dual substrates without the primary carbon source. The biodegradation yields of the OTC, BaP were discussed with sole-substrate systems and with the dual substrate system in the presence of the primary substrate. The maximum OTC and BaP yields were 93% in Run 3 with the primary substrate, while the maximum BaP and OTC yields were 95%, 98% in Run 3 without the primary substrate. A dual form of the Monod was found to adequately predict the substrate interactions in the binary mixture of OTC and BaP using only the parameters derived from batch experiments. At low BaP (4 mg L(-1)) and OTC (40 mg L(-1)) concentrations, a non-competitive inhibition does not affect the binding of the substrate and so the K(s) were was not affected while the µ(max) was lowered. At high BaP (10 mg L(-1)) and OTC (100 mg L(-1)) concentrations, the BaP and OTC were biodegraded according to competitive inhibition with increased K(s) while µ(max) was not affected. BaP and OTC were biodegraded according to Haldane at high concentrations (>10 mg L(-1) for BaP, 100 mg L(-1) OTC) where they were used as the sole substrate.

  18. Molecular characteristics of bap-positive Staphylococcus aureus strains from dairy cow mastitis.

    Science.gov (United States)

    Snel, Gustavo G M; Monecke, Stefan; Ehricht, Ralf; Piccinini, Renata

    2015-08-01

    The biofilm-associated protein (Bap) of Staphylococcus aureus is a high molecular weight cell-wall-anchored protein involved in biofilm formation, first described in bovine mastitis strains from Spain. So far, studies regarding Bap were mainly based on the Spanish strain V329 and its mutants, but no information on the genetic variability of bap-positive Staph. aureus strains is yet available in the literature. The present study investigated the molecular characteristics of 8 bap-positive Staph. aureus strains from subclinical bovine mastitis, isolated in 5 herds; somatic cell counts (SCC) of milk samples were also registered. Strains were characterised using MLST, SPA typing and microarray and the results were compared with V329. All isolates from this study and V329 were assigned to ST126, t605, but some molecular differences were observed. Only herd A and B strains harboured the genes for β-lactams resistance; the leukocidin D/E gene, a type I site-specific deoxyribonuclease subunit, 3rd locus gene and serin-protease A and B were carried by all strains, but not by V329, while serin-protease E was absent in V329 and in another isolate. Four isolates and V329 harboured the fibronectin-binding protein B gene. SCC showed the highest value in the milk sample affected by the only strain carrying all the virulence factors considered. Potential large variability of virulence was evidenced among V329 and all bap-positive Staph. aureus strains considered: the carriage of fnb could enhance the accumulation of biofilm, but the lack of lukD/E and splA, B or E might decrease the invasiveness of strain.

  19. BAP1 Immunohistochemistry and p16 FISH in the Diagnosis of Sarcomatous and Desmoplastic Mesotheliomas.

    Science.gov (United States)

    Hwang, Harry C; Pyott, Shawna; Rodriguez, Stephanie; Cindric, Ashlie; Carr, April; Michelsen, Carmen; Thompson, Kim; Tse, Christopher H; Gown, Allen M; Churg, Andrew

    2016-05-01

    The separation of sarcomatous and desmoplastic mesotheliomas from benign organizing pleuritis can be morphologically very difficult. Deletion of p16 (CDKN2A) by fluorescence in situ hybridization (FISH) testing appears to be a reliable marker of malignancy in mesothelial proliferations, and more recently it has been reported that, in this setting, loss of BAP1 by immunohistochemistry is only seen in malignant mesotheliomas. To determine how useful these tests are with sarcomatous and desmoplastic mesotheliomas, we examined 20 such tumors. Loss of BAP1 was seen in 3/20 (15%) and deletion of p16 by FISH was seen in 16/20 (80%) cases. Loss of one or the other marker was observed in 17/20 (85%). We also examined 13 sarcomatoid carcinomas, an important differential diagnosis of sarcomatoid mesotheliomas, and found that BAP1 was never lost, but p16 was deleted in 3/11 (27%). We conclude that: (1) BAP1 immunohistochemistry is relatively insensitive in the context of sarcomatous and desmoplastic mesotheliomas, but as a matter of time and cost efficiency may nonetheless be a useful first approach to the problem; (2) deletion of p16 by FISH is considerably more sensitive, but there remain a proportion of cases in which p16 is not deleted; (3) a small improvement in sensitivity can be achieved by using both markers; (4) in the context of a spindle cell malignant tumor in the pleura or peritoneum, which morphologically might be a metastatic sarcomatoid carcinoma or a mesothelioma, the finding of BAP1 loss favors mesothelioma, but p16 FISH cannot be used to separate sarcomatous mesotheliomas from sarcomatoid carcinomas. PMID:26900815

  20. Molecular characteristics of bap-positive Staphylococcus aureus strains from dairy cow mastitis.

    Science.gov (United States)

    Snel, Gustavo G M; Monecke, Stefan; Ehricht, Ralf; Piccinini, Renata

    2015-08-01

    The biofilm-associated protein (Bap) of Staphylococcus aureus is a high molecular weight cell-wall-anchored protein involved in biofilm formation, first described in bovine mastitis strains from Spain. So far, studies regarding Bap were mainly based on the Spanish strain V329 and its mutants, but no information on the genetic variability of bap-positive Staph. aureus strains is yet available in the literature. The present study investigated the molecular characteristics of 8 bap-positive Staph. aureus strains from subclinical bovine mastitis, isolated in 5 herds; somatic cell counts (SCC) of milk samples were also registered. Strains were characterised using MLST, SPA typing and microarray and the results were compared with V329. All isolates from this study and V329 were assigned to ST126, t605, but some molecular differences were observed. Only herd A and B strains harboured the genes for β-lactams resistance; the leukocidin D/E gene, a type I site-specific deoxyribonuclease subunit, 3rd locus gene and serin-protease A and B were carried by all strains, but not by V329, while serin-protease E was absent in V329 and in another isolate. Four isolates and V329 harboured the fibronectin-binding protein B gene. SCC showed the highest value in the milk sample affected by the only strain carrying all the virulence factors considered. Potential large variability of virulence was evidenced among V329 and all bap-positive Staph. aureus strains considered: the carriage of fnb could enhance the accumulation of biofilm, but the lack of lukD/E and splA, B or E might decrease the invasiveness of strain. PMID:25850658

  1. CDIP1-BAP31 Complex Transduces Apoptotic Signals from Endoplasmic Reticulum to Mitochondria under Endoplasmic Reticulum Stress

    Directory of Open Access Journals (Sweden)

    Takushi Namba

    2013-10-01

    Full Text Available Resolved endoplasmic reticulum (ER stress response is essential for intracellular homeostatic balance, but unsettled ER stress can lead to apoptosis. Here, we show that a proapoptotic p53 target, CDIP1, acts as a key signal transducer of ER-stress-mediated apoptosis. We identify B-cell-receptor-associated protein 31 (BAP31 as an interacting partner of CDIP1. Upon ER stress, CDIP1 is induced and enhances an association with BAP31 at the ER membrane. We also show that CDIP1 binding to BAP31 is required for BAP31 cleavage upon ER stress and for BAP31-Bcl-2 association. The recruitment of Bcl-2 to the BAP31-CDIP1 complex, as well as CDIP1-dependent truncated Bid (tBid and caspase-8 activation, contributes to BAX oligomerization. Genetic knockout of CDIP1 in mice leads to impaired response to ER-stress-mediated apoptosis. Altogether, our data demonstrate that the CDIP1/BAP31-mediated regulation of mitochondrial apoptosis pathway represents a mechanism for establishing an ER-mitochondrial crosstalk for ER-stress-mediated apoptosis signaling.

  2. Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop.

    Science.gov (United States)

    Baghel, S; Bansal, Y K

    2014-04-01

    Apical and axillary buds of Guizotia abyssinica Cass., isolated from seedlings raised in vitro, were cultured. High frequency of shoot regeneration was achieved on MS medium with BAP (1 mgl(-1)). Effect of BAP, Kn and GA3 applied successively in culture on shoot regeneration and flower bud formation has been studied. The shoots differentiated in cultures elongated on this medium. These rooted subsequently on half strength MS medium. The shoots flowered in vitro on MS medium with a combination of BAP (0.1mgl(-1)) + GA3 (0.1 mgl(-1)). The plantlets thus formed were successfully hardened with 90 % survival.

  3. BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping.

    Science.gov (United States)

    Vu, Giang T H; Dear, Paul H; Caligari, Peter D S; Wilkinson, Mike J

    2010-02-08

    Physical and linkage mapping underpin efforts to sequence and characterize the genomes of eukaryotic organisms by providing a skeleton framework for whole genome assembly. Hitherto, linkage and physical "contig" maps were generated independently prior to merging. Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline. Using Arabidopsis thaliana as an exemplar, a set of 40 Sequence Tagged Site (STS) markers spanning approximately 10% of chromosome 4 were simultaneously assembled onto a BAP map compiled using both a series of BAC pools each comprising 0.7x genome coverage and dilute (0.7x genome) samples of sheared genomic DNA. The resultant BAP map overcomes the need for polymorphic loci to separate genetic loci by recombination and allows physical mapping in segments of suppressed recombination that are difficult to analyze using traditional mapping techniques. Even virtual "BAC-HAPPY-mapping" to convert BAC landing data into BAC linkage contigs is possible.

  4. Immunofluorescence detection and localization of B[a]P and TCDD in earthworm tissues.

    Science.gov (United States)

    Sforzini, Susanna; Moore, Michael N; Boeri, Marta; Benfenati, Emilio; Colombo, Andrea; Viarengo, Aldo

    2014-07-01

    An immunohistochemical method using antibodies against polycyclic aromatic hydrocarbons (PAHs) and dioxins was developed on frozen tissue sections of the earthworm Eisenia andrei exposed to environmentally relevant concentrations of benzo[a]pyrene (B[a]P) (0.1, 10, 50 ppm) and 2,3,7,8-tetrachloro-dibenzo-para-dioxin (TCDD) (0.01, 0.1, 2 ppb) in spiked standard soils. The concentrations of B[a]P and TCDD in E. andrei exposed to the same conditions were also measured using analytical chemical procedures. The results demonstrated that tissues of worms exposed to even minimal amount of B[a]P and TCDD reacted positively and specifically to anti-PAHs and -dioxins antibody. Immunofluorescence revealed a much more intense staining for the gut compared to the body wall; moreover, positively immunoreactive amoeboid coelomocytes were also observed, i.e. cells in which we have previously demonstrated the occurrence of genotoxic damage. The double immunolabelling with antibodies against B[a]P/TCDD and the lysosomal enzyme cathepsin D demonstrated the lysosomal accumulation of the organic xenobiotic compounds, in particular in the cells of the chloragogenous tissue as well as in coelomocytes, involved into detoxification and protection of animals against toxic chemicals. The method described is timesaving, not expensive and easily applicable. PMID:24412505

  5. Palaeoenvironmental history of Bap-Malar and Kanod playas of western Rajasthan, Thar desert

    Indian Academy of Sciences (India)

    B C Deotare; M D Kajale; S N Rajaguru; S Kusumgar; A J T Jull; J D Donahue

    2004-09-01

    Two playas in the arid core of the western margin of the Thar desert viz., Bap-Malar and Kanod, have been investigated using palynology, geomorphology, archaeology, AMS-radiocarbon dating, stable isotopes, evaporite mineralogy and geoarchaeology. The principal objective was to obtain a reliable lithostratigraphy of the playa sediments. These are about 7m thick in the Bap-Malar and < 2.5 to 3m thick in the Kanod. AMS 14C dates of < 15 ka BP on pollen from sediment layers indicates that the Bap-Malar playa possibly existed even during the LGM. These playas were full of water during the early Holocene (8 ka BP — 5.5 ka BP) and were ephemeral during the Pleistocene- Holocene transition and early to mid to late Holocene. The playas dried almost 1000 years earlier than those occurring on the eastern margin. Pollen of graminaceae, chenopodiaceae / amaranthaceae, cyperaceae etc. and evaporite minerals like gypsum, halite in the profiles indicate that the playas were surrounded by vegetation dominated by grass and that, they remained brackish to saline even during the mid Holocene, lake full stage. Stable dune surfaces, pediments with regoliths, and gravelly channels of ephemeral streams provided a favorable geomorphic niche for nomadic human activity since ∼7 ka BP. Though local ecological factors have played an important role in the evolution of the playas, the winter rains, connected with northwesterly depressions, most likely played a vital role in maintaining these playas.

  6. Concentrações de citocinina e carvão ativado na micropropagação de pimenta-do-reino Cytokynin concentrations and activated charcoal on black pepper micropropagation

    Directory of Open Access Journals (Sweden)

    Elisa Ferreira Moura

    2008-02-01

    Full Text Available A pimenta-do-reino (Piper nigrum tem grande importância para a economia do Norte do Brasil. A pimenta-do-reino é propagada principalmente por estaquia que, além de ser pouco eficiente, pode possibilitar a propagação da doença fusariose. Dessa forma, testes para verificar a eficiência da micropropagação são pertinentes. O objetivo do trabalho foi testar concentrações de BAP - 6-benzilaminopurina (0,5, 1,5, 3,0 e 4,5mg L-1 e a adição de carvão ativado a 0,2% ao meio de cultura na micropropagação de pimenta-do-reino. O meio de cultura foi composto de sais e vitaminas de Murashige & Skoog, 3% de sacarose e 8g L-1 de ágar. Foram utilizados como explantes ápices caulinares de plântulas da cultivar "Bragantina" germinadas in vitro. Os tratamentos foram avaliados em esquema fatorial 4x2, em delineamento inteiramente casualizado. Após 45 dias no estabelecimento, foi avaliado o número de brotos, mas não foi observada diferença significativa entre os tratamentos, considerando-se os dois fatores e a interação entre eles. Após 45 dias no primeiro subcultivo, foram avaliados o número de brotos e de explantes (segmentos nodais. A interação entre BAP e carvão ativado teve efeito significativo para número de brotos e de explantes, sendo que o carvão influenciou o efeito das concentrações de BAP. A concentração de 0,5mg L-1 de BAP na ausência de carvão ativado gerou maior número de brotos e de explantes. O carvão ativado não é necessário na fase de proliferação de gemas de pimenta-do-reino da cultivar "Bragantina".Black pepper (Piper nigrum has great importance for the Northern Brazil’s economy. Black pepper is mainly propagated by mass propagation, which is not very efficient and may spread fusariosis. Thus, micropropagation efficiency tests are pertinent. The objective of this research paper was to test BAP - 6-benzilaminopurine concentrations (0.5, 1.5, 3.0 and 4.5mg L-1 and activated charcoal at 0.2% addition to

  7. EROD activity and genotoxicity in the seabob shrimp Xiphopenaeus kroyeri exposed to benzo[a]pyrene (BaP) concentrations.

    Science.gov (United States)

    da Silva Rocha, Arthur José; Gomes, Vicente; Rocha Passos, Maria José de Arruda Campos; Hasue, Fabio Matsu; Alves Santos, Thaís Cruz; Bícego, Márcia Caruso; Taniguchi, Satie; Van Ngan, Phan

    2012-11-01

    Seabob shrimp Xiphopenaeus kroyeri is a marine species that lives in shallow waters of coastal environments, often impacted by polycyclic aromatic hydrocarbons (PAH) pollution. In the present study, seabob shrimp were exposed for 96 h to benzo[a]pyrene (BaP) at the nominal concentrations of 100, 200, 400 and 800 microg-L(-1). Animals of the control groups were exposed either to clean water or to the BaP-carrier (DMSO). At the end of the exposures, muscle tissues were sampled for BaP uptake assessment and hepatopancreas and hemolymph for EROD enzyme activity and hemocytes DNA damage, respectively. EROD activity and DNA damage increased significantly as a function of BaP exposure concentrations. Significant correlations between BaP uptake and both EROD activity and DNA damage suggest that they can be used as suitable tools for integrated levels of study on the biomarkers of PAH exposure.

  8. Preparation of BAP Composite Particles and Their Effects on Rheological Properties of HTPB/B/AP Slurries

    Science.gov (United States)

    Yun-Fei, Liu; Yu, Chen; Liang, Shi; Wei-Shang, Yao

    2014-04-01

    Composite particles (BAP) of boron (B) coated with ammonium perchlorate (AP) were prepared by recrystallization and their structures were characterized using Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and laser particle size analyses. The results indicated that AP was well coated on the boron surface. The effects of different contents of BAP coated with different amounts of AP on the rheological properties of HTPB/B/AP composite slurries were also studied. The results show that addition of BAP distinctly decreased the slurries' viscosities and improved their processing properties, which were more obvious when 9.73% AP was coated on the surface of boron and the amount of BAP was 20%.

  9. BAP-65 and CURB-65 scores in prediction need for mechanical ventilation in patients with AECOPD%BAP-65和CURB-65评分预测AECOPD患者行机械通气的研究

    Institute of Scientific and Technical Information of China (English)

    蒋云; 崔妙玲; 杨祺; 刘小英

    2015-01-01

    Objective To study the scores of BAP-65 and CURB-65 in predicting need for mechanical venti-lation (MV)in patients with AECOPD.Methods A total of 1 71 patients with AECOPD were selected,and the scores of BAP-65 and CURB-65 were compared between MV patients and non-MV patients.The prediction value of the scores on mechanical ventilation was evaluated through ROC curve.Results The scores of BAP-65 and CURB-65 were higher in the MV group than in the non-MV group (P<0.01 ).The area under ROC curve of BAP-65 and CURB-65 was both higher than 0.7,but BAP-65 was superior to CURB-65 in the prediction of MV need.Conclu-sion The scores of BAP-65 and CURB-65 show strong capability of predicting MV need,and BAP-65 is more accu-rate and suitable than CURB-65 is.%目的:探讨BAP-65及CURB-65评分在预测慢性阻塞性肺疾病急性加重期(AECOPD)患者行机械通气的应用。方法以呼吸内科、内科ICU病区的171例AECOPD患者为研究对象,比较机械通气患者(MV组)和非机械通气患者(非MV组)的BAP-65和CURB-65评分;构建ROC曲线分析BAP-65和CURB-65评分对AECOPD患者行机械通气的预测价值。结果 MV组患者的BAP-65评分的风险等级和CURB-65评分的分值显著高于非MV组(P<0.01);BAP-65和CURB-65评分的ROC曲线下面积(AUROC)均大于0.7,能较好预测 AECOPD 患者行机械通气,但 BAP-65评分(AUROC =0.843)优于 CURB-65评分(AUROC =0.728)。结论 BAP-65和CURB-65评分均具有较好的分辨度,BAP-65评分准确性高于CURB-65评分,更适用于AECOPD患者行机械通气的预测。

  10. Jumping the gun: smoking constituent BaP causes premature primordial follicle activation and impairs oocyte fusibility through oxidative stress.

    Science.gov (United States)

    Sobinoff, A P; Pye, V; Nixon, B; Roman, S D; McLaughlin, E A

    2012-04-01

    Benzo(a)pyrene (BaP) is an ovotoxic constituent of cigarette smoke associated with pre-mature ovarian failure and decreased rates of conception in IVF patients. Although the overall effect of BaP on female fertility has been documented, the exact molecular mechanisms behind its ovotoxicity remain elusive. In this study we examined the effects of BaP exposure on the ovarian transcriptome, and observed the effects of in vivo exposure on oocyte dysfunction. Microarray analysis of BaP cultured neonatal ovaries revealed a complex mechanism of ovotoxicity involving a small cohort of genes associated with follicular growth, cell cycle progression, and cell death. Histomorphological and immunohistochemical analysis supported these results, with BaP exposure causing increased primordial follicle activation and developing follicle atresia in vitro and in vivo. Functional analysis of oocytes obtained from adult Swiss mice treated neonatally revealed significantly increased levels of mitochondrial ROS/lipid peroxidation, and severely reduced sperm-egg binding and fusion in both low (1.5mg/kg/daily) and high (3mg/kg/daily) dose treatments. Our results reveal a complex mechanism of BaP induced ovotoxicity involving developing follicle atresia and accelerated primordial follicle activation, and suggest short term neonatal BaP exposure causes mitochondrial leakage resulting in reduced oolemma fluidity and impaired fertilisation in adulthood. This study highlights BaP as a key compound which may be partially responsible for the documented effects of cigarette smoke on follicular development and sub-fertility.

  11. Identification of bap and icaA genes involved in biofilm formation in coagulase negative staphylococci isolated from feline conjunctiva.

    Science.gov (United States)

    Płoneczka-Janeczko, Katarzyna; Lis, Paweł; Bierowiec, Karolina; Rypuła, Krzysztof; Chorbiński, Paweł

    2014-12-01

    Bap and icaA genes are commonly known to be involved in the biofilm formation. The prevalence of bap and icaA genes and biofilm formation was determined in conjunctival isolates of coagulase negative staphylococci (CNS) collected from cats. The study was conducted on 90 archival CNS isolates collected from feline conjunctiva obtained from clinically healthy cats and cats with ocular problems. Biofilm formation was examined using the microtiter plate (MTP) method. The prevalence of icaA and bap genes was determined using polymerase chain reaction (PCR). Genetic profiles of the bap-positive isolates were examined using the modified random amplified polymorphic DNA (RAPD) method. Of the 90 CNS isolates investigated, 58.9% (53/90) were confirmed to form biofilms on a polystyrene plate after 24 h, and the intensity of the biofilm production varied strongly between positive strains. Among the biofilm-producing isolates, 24.5% (13/53) carried the icaA gene and 3.8% (2/53) carried the bap gene. Among the isolates that did not produce biofilms, the icaA gene and bap gene were detected in 8.1% (3/37) and 2.7% (1/37) of isolates, respectively. This is the first report demonstrating that CNS isolated from feline conjunctiva can potentially be a bap gene reservoir. Preliminary comparison of the genetic profiles of three bap-positive isolates collected from cats showed that each of the isolates has a different genetic background with a high similarity with the human strain of S. epidermidis.

  12. Enhanced Bayesian modelling in BAPS software for learning genetic structures of populations

    Directory of Open Access Journals (Sweden)

    Sirén Jukka

    2008-12-01

    Full Text Available Abstract Background During the most recent decade many Bayesian statistical models and software for answering questions related to the genetic structure underlying population samples have appeared in the scientific literature. Most of these methods utilize molecular markers for the inferences, while some are also capable of handling DNA sequence data. In a number of earlier works, we have introduced an array of statistical methods for population genetic inference that are implemented in the software BAPS. However, the complexity of biological problems related to genetic structure analysis keeps increasing such that in many cases the current methods may provide either inappropriate or insufficient solutions. Results We discuss the necessity of enhancing the statistical approaches to face the challenges posed by the ever-increasing amounts of molecular data generated by scientists over a wide range of research areas and introduce an array of new statistical tools implemented in the most recent version of BAPS. With these methods it is possible, e.g., to fit genetic mixture models using user-specified numbers of clusters and to estimate levels of admixture under a genetic linkage model. Also, alleles representing a different ancestry compared to the average observed genomic positions can be tracked for the sampled individuals, and a priori specified hypotheses about genetic population structure can be directly compared using Bayes' theorem. In general, we have improved further the computational characteristics of the algorithms behind the methods implemented in BAPS facilitating the analyses of large and complex datasets. In particular, analysis of a single dataset can now be spread over multiple computers using a script interface to the software. Conclusion The Bayesian modelling methods introduced in this article represent an array of enhanced tools for learning the genetic structure of populations. Their implementations in the BAPS software are

  13. Mesothelioma patient derived tumor xenografts with defined BAP1 mutations that mimic the molecular characteristics of human malignant mesothelioma

    International Nuclear Information System (INIS)

    The development and evaluation of new therapeutic approaches for malignant mesothelioma has been sparse due, in part, to lack of suitable tumor models. We established primary mesothelioma cultures from pleural and ascitic fluids of five patients with advanced mesothelioma. Electron microscopy and immunohistochemistry (IHC) confirmed their mesothelial origin. Patient derived xenografts were generated by injecting the cells in nude or SCID mice, and malignant potential of the cells was analyzed by soft agar colony assay. Molecular profiles of the primary patient tumors, early passage cell cultures, and patient derived xenografts were assessed using mutational analysis, fluorescence in situ hybridization (FISH) analysis and IHC. Primary cultures from all five tumors exhibited morphologic and IHC features consistent to those of mesothelioma cells. Mutations of BAP1 and CDKN2A were each detected in four tumors. BAP1 mutation was associated with the lack of expression of BAP1 protein. Three cell cultures, all of which were derived from BAP1 mutant primary tumors, exhibited anchorage independent growth and also formed tumors in mice, suggesting that BAP1 loss may enhance tumor growth in vivo. Both early passage cell cultures and mouse xenograft tumors harbored BAP1 mutations and CDKN2A deletions identical to those found in the corresponding primary patient tumors. The mesothelioma patient derived tumor xenografts with mutational alterations that mimic those observed in patient tumors which we established can be used for preclinical development of novel drug regimens and for studying the functional aspects of BAP1 biology in mesothelioma. The online version of this article (doi:10.1186/s12885-015-1362-2) contains supplementary material, which is available to authorized users

  14. Efeito de citocininas na senescência e abscisão foliar durante o cultivo in vitro de Annona glabra L. Effect of cytokinins on senescence and foliar abscision during in vitro Annona glabra L. cultivation

    Directory of Open Access Journals (Sweden)

    Lenaldo Muniz de Oliveira

    2007-04-01

    Full Text Available A micropropagação de anonáceas tem sido limitada pela abscisão foliar precoce nas brotações, o que dificulta a manutenção e o desenvolvimento das plantas in vitro. Esse fato se deve, principalmente, ao acúmulo de etileno nos tubos fechados e à relação etileno/citocinina nas folhas. Assim sendo, avaliaram-se o efeito de fontes de citocinina sobre o retardo da senescência foliar em brotações de Annona glabra L. e suas implicações sobre o seu desenvolvimento. Segmentos nodais foram cultivados em tubo de ensaio contendo 15 mL do meio WPM, suplementado com 30g L-1 de sacarose, 500mg L-1 de benomyl e 1g L-1 de carvão ativado. A esse meio adicionaram-se 6-benzilaminopurina, thidiazuron, cinetina e zeatina, todos na concentração de 1mg L-1. Decorridos 45 dias após a inoculação, plantas foram submetidas à senescência em ambiente escuro, por um período de 9 dias, coletando-se folhas a cada três dias para quantificação de clorofila "a", clorofila "b", carotenóides, proteínas e açúcares solúveis totais. No final das fases de multiplicação e enraizamento, quantificaram-se a matéria seca, a área foliar e o número de folhas que sofreram abscisão nas plantas que não foram submetidas à senescência. Adotou-se o delineamento em blocos casualizados, sendo que cada período de senescência constituiu um bloco, com quatro repetições por tratamento. Os resultados mostraram que cinetina e zeatina, seguidas de thidiazuron e 6-benzilaminopurina, preservam maior teor de clorofilas "a", "b" e de carotenóides durante todo o período de senescência induzida. 6-benzilaminopurina e cinetina promoveram maior retenção da área foliar durante as fases de multiplicação e enraizamento de Annona glabra L.The micropropagation of Annonaceae has been limited by early foliar abscission in shoots, which makes the maintenance and development of plants in vitro environment difficult. This is mainly due to ethylene accumulation in closed

  15. BaP (PAH) air quality modelling exercise over Zaragoza (Spain) using an adapted version of WRF-CMAQ model.

    Science.gov (United States)

    San José, Roberto; Pérez, Juan Luis; Callén, María Soledad; López, José Manuel; Mastral, Ana

    2013-12-01

    Benzo(a)pyrene (BaP) is one of the most dangerous PAH due to its high carcinogenic and mutagenic character. Because of this reason, the Directive 2004/107/CE of the European Union establishes a target value of 1 ng/m(3) of BaP in the atmosphere. In this paper, the main aim is to estimate the BaP concentrations in the atmosphere by using last generation of air quality dispersion models with the inclusion of the transport, scavenging and deposition processes for the BaP. The degradation of the particulated BaP by the ozone has been considered. The aerosol-gas partitioning phenomenon in the atmosphere is modelled taking into a count that the concentrations in the gas and the aerosol phases. If the pre-existing organic aerosol concentrations are zero gas/particle equilibrium is established. The model has been validated at local scale with data from a sampling campaign carried out in the area of Zaragoza (Spain) during 12 weeks.

  16. Investigations on critical parameters, growth, structural and spectral studies of beta-alaninium picrate (BAP) single crystals

    Science.gov (United States)

    Shanthi, D.; Selvarajan, P.; Perumal, S.

    2014-12-01

    Beta-alaninium picrate (BAP) salt has been synthesized and the solubility of the synthesized sample in double distilled water was determined at different temperatures. Solution stability was studied by observing the metastable zone width by employing the polythermal method. Induction period values for different supersaturation ratios at room temperature were determined based on the isothermal method. The nucleation parameters such as critical radius, critical free energy change, interfacial tension, and nucleation rate have been estimated for BAP salt on the basis of the classical nucleation theory. The lattice parameters of the grown BAP crystal were determined using the x-ray diffraction (XRD) technique. The reflection planes of the sample were confirmed by the powder XRD study and diffraction peaks were indexed. Fourier transform infrared spectroscopy and Fourier transform-Raman studies were used to confirm the presence of various functional groups in the BAP crystal. The nonlinear optical property of the grown crystal was studied using the Kurtz-Perry powder technique. UV-visible spectral studies were carried out to understand optical transparency and the type of band gap of the grown BAP crystal.

  17. Investigations on critical parameters, growth, structural and spectral studies of beta-alaninium picrate (BAP) single crystals

    International Nuclear Information System (INIS)

    Beta-alaninium picrate (BAP) salt has been synthesized and the solubility of the synthesized sample in double distilled water was determined at different temperatures. Solution stability was studied by observing the metastable zone width by employing the polythermal method. Induction period values for different supersaturation ratios at room temperature were determined based on the isothermal method. The nucleation parameters such as critical radius, critical free energy change, interfacial tension, and nucleation rate have been estimated for BAP salt on the basis of the classical nucleation theory. The lattice parameters of the grown BAP crystal were determined using the x-ray diffraction (XRD) technique. The reflection planes of the sample were confirmed by the powder XRD study and diffraction peaks were indexed. Fourier transform infrared spectroscopy and Fourier transform–Raman studies were used to confirm the presence of various functional groups in the BAP crystal. The nonlinear optical property of the grown crystal was studied using the Kurtz–Perry powder technique. UV–visible spectral studies were carried out to understand optical transparency and the type of band gap of the grown BAP crystal. (paper)

  18. Diodos emissores de luz e concentrações de BAP na multiplicação in vitro de morangueiro Light-emitting diodes and BAP concentrations in the in vitro strawberry multiplication

    Directory of Open Access Journals (Sweden)

    Paulo Sérgio Gomes da Rocha

    2010-09-01

    Full Text Available Realizou-se um estudo para avaliar o efeito de fontes de luz na micropropagação de morangueiro, com níveis crescentes de BAP no meio de cultivo. Para tanto, inocularam-se gemas de brotações da cultivar 'Sabrosa' em meio MS com 30g L-1 de sacarose, 100mg L-1 de mio-inositol, 7g L-1 de ágar e BAP (0; 0,3; 0,6; 0,9; e 1,5mg L-1, em pH 5,8. Os explantes foram cultivados a 25+2°C, com 16 horas de fotoperíodo e luminosidade de 20µmol m-2 s-1, esta última fornecida por diferentes fontes de luz (LED azul-EDEB 3LA1, LED verde-EDET 3LA1, LED vermelho-EDER 3LA3, lâmpada fluorescente Growlux e lâmpada fluorescente branca. Os tratamentos foram dispostos em delineamento inteiramente ao acaso em um fatorial 5x5 (concentrações de BAP x fontes de luz, com seis repetições. O experimento foi repetido em três subcultivos sucessivos de 35 dias cada. Nestes avaliaram-se o número de brotações por explante e a altura das brotações. Ao final do terceiro subcultivo, determinaram-se, ainda, as concentrações de carotenoides e de clorofilas a e b, independentemente do nível de BAP. Maior número de brotações por explante foi obtido sob LEDs vermelhos e verdes. Concentrações de BAP no meio de cultura entre 0,82 e 1,22mg L-1, dependendo da fonte de luz, proporcionaram maior multiplicação in vitro de brotações. Sob todas as fontes de luz foram obtidas brotações de maior comprimento em meio isento de BAP. Brotações cultivadas sob LEDs vermelhos apresentaram maior quantidade de pigmentos fotossintéticos, enquanto aquelas sob LEDs verdes e lâmpadas Growlux apresentaram a menor.The objective of this research was to evaluate the effect of different sources of light in strawberry micropropagation, under increasing levels of BAP in culture medium. 'Sabrosa' shoots were inoculated in MS medium supplemented with 30g L-1 sucrose, 100mg L-1 myo-inositol, 7 agar g L-1 and BAP (0; 0.3; 0.6; 0.9; e 1.5mg L-1, pH 5.8. The explants were cultivated at 25

  19. Trans-membrane transport of fluoranthene by Rhodococcus sp. BAP-1 and optimization of uptake process.

    Science.gov (United States)

    Li, Yi; Wang, Hongqi; Hua, Fei; Su, Mengyuan; Zhao, Yicun

    2014-03-01

    The mechanism of transport of (14)C-fluoranthene by Rhodococcus sp. BAP-1, a Gram-positive bacterium isolated from crude oil-polluted soil, was examined. Our finding demonstrated that the mechanism for fluoranthene travel across the cell membrane in Rhodococcus sp. BAP-1 requires energy. Meanwhile, the transport of fluoranthene involves concurrent catabolism of (14)C, that leading to the generation of significant amount of (14)CO2. Combined with trans-membrane transport dynamic and response surface methodology, a significant influence of temperature, pH and salinity on cellular uptake rate was screened by Plackett-Burman design. Then, Box-Behnken design was employed to optimize and enhanced the trans-membrane transport process. The results predicted by Box-Behnken design indicated that the maximum cellular uptake rate of fluoranthene could be achieve to 0.308μmolmin(-1)mg(-1)·protein (observed) and 0.304μmolmin(-1)mg(-1)·protein (predicted) when the initial temperature, pH and salinity were set at 20°C, 9% and 1%, respectively.

  20. Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31

    Energy Technology Data Exchange (ETDEWEB)

    Mukasa, Takashi; Santelli, Eugenio [Program on Infectious Diseases, Center for Inflammation and Infectious Diseases, The Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037 (United States); Reed, John C. [Program on Apoptosis, Cancer Center, The Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037 (United States); Pascual, Jaime, E-mail: pascual@burnham.org [Program on Infectious Diseases, Center for Inflammation and Infectious Diseases, The Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037 (United States)

    2007-04-01

    A leucine-zipper with properties as apoptotic regulator in the ER has been crystallized. X-ray data to 2.5 Å resolution were collected, molecular replacement solutions were identified and refinement has been started. B-cell receptor-associated protein 31 (Bap31) is an integral membrane protein located in the endoplasmic reticulum (ER) that participates in the transport and quality control of membrane proteins and plays a role in determining cell sensitivity to ER stress and apoptosis. Its cytoplasmic region contains two target sites for caspase cleavage in certain apoptotic pathways. Here, the subcloning, expression, purification and crystallization of the Homo sapiens Bap31 leucine-zipper C-terminal fragment, which spans residues Gly160–Glu246, are reported. An N-terminally His-tagged protein was overexpressed in Escherichia coli and purified by chromatographic methods. X-ray diffraction data were collected in-house to 2.5 Å resolution. Crystals belong to space group P6{sub 1}22/P6{sub 5}22, with unit-cell parameters a = b = 70.7, c = 80.6 Å. Data analysis indicates the presence of one molecule per asymmetric unit.

  1. Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31

    International Nuclear Information System (INIS)

    A leucine-zipper with properties as apoptotic regulator in the ER has been crystallized. X-ray data to 2.5 Å resolution were collected, molecular replacement solutions were identified and refinement has been started. B-cell receptor-associated protein 31 (Bap31) is an integral membrane protein located in the endoplasmic reticulum (ER) that participates in the transport and quality control of membrane proteins and plays a role in determining cell sensitivity to ER stress and apoptosis. Its cytoplasmic region contains two target sites for caspase cleavage in certain apoptotic pathways. Here, the subcloning, expression, purification and crystallization of the Homo sapiens Bap31 leucine-zipper C-terminal fragment, which spans residues Gly160–Glu246, are reported. An N-terminally His-tagged protein was overexpressed in Escherichia coli and purified by chromatographic methods. X-ray diffraction data were collected in-house to 2.5 Å resolution. Crystals belong to space group P6122/P6522, with unit-cell parameters a = b = 70.7, c = 80.6 Å. Data analysis indicates the presence of one molecule per asymmetric unit

  2. The Study of Inhibition Effects Satureja khuzestaniea Essence against Gene Expression bap Acinetobacter baumannii with Real time PCR Technique

    Directory of Open Access Journals (Sweden)

    Abbas Bahador

    2015-04-01

    Full Text Available Background and Aim: Acinetobacter baumannii a major hospital pathogens and causes outbreaks of infections and associated to nosocomial infections, including bacteremia, pneumonia, meningitis, urinary tract infection, and wound infections. Satureja khuzestaniea province the group Nnayyan and Carvacrol and thymol are antimicrobial activity of this plant. Biofilm-related genes (bap-specific proteins on the cell surface generate a direct role in biofilm formation and infection of the bacteria is eliminated. The aim of this study was to investigate the effect of alcoholic extract Satureja khuzestaniea in gene expression (bap was A. baumanii if it can be as effective as a complementary treatment or therapy in infections caused by these bacteria and inhibit virulence genes used. Materials and Methods: In this study the effect of Satureja khuzestanica essence, an endemic plant of Iran, on the expression level of bap gene in A. baumannii   were investigated. For this purpose, MIC was determined for A. baumanii   . Then, bacteria were treated with S. khuzistanica essence. The bap genes expression in treated and non-treated bacteria, before and after treatment was evaluated using Real time PCR technique. Results: Surprisingly, the expression level of bap gene was decreased in the presence of S. khuzestanica. However, the expression of DNA gyrA gene that was used as an internal control was not altered before and after treatment with this herb. Conclusions: Based on the results, S. khuzestanica could play a, major role in lowering the A. baumannii   resistance to drugs, by reducing bap gene expression. According to results of current research we hope in future be used it to the clinic with a wider range as a complementary therapy and also for surgery operation.

  3. Influência da concentração de BAP e AG3 no desenvolvimento in vitro de mandioquinha-salsa Influence of BAP and GA3 concentration on the in vitro development of arracacha

    Directory of Open Access Journals (Sweden)

    Nuno R. Madeira

    2005-12-01

    Full Text Available Foram realizados dois experimentos com o objetivo de avaliar o efeito das concentrações de BAP e de AG3 no desenvolvimento in vitro de mandioquinha-salsa. Foram avaliadas as concentrações de 0,0; 0,2 e 0,4 mg L-1 de BAP e de 0,0; 0,125 e 0,250 mg L-1 de AG3 no desenvolvimento de ápices caulinares com cerca de 2 mm das cultivares Amarela de Senador Amaral e Amarela Comum. A interação cultivares x concentração de BAP não foi significativa para nenhuma das características avaliadas (P>0,0617. O aumento na concentração de BAP reduziu a formação de calos; entretanto, promoveu proporcionalmente redução no desenvolvimento da parte aérea. A concentração de BAP que apresentou os melhores resultados foi em torno de 0,3 mg L-1 de BAP, conciliando diâmetro de calo relativamente reduzido e bom desenvolvimento da parte aérea, cerca de 4,5 brotos com altura média de 43 mm. A interação cultivares x concentração de AG3 não foi significativa para nenhuma das características avaliadas (P>0,4310. O aumento na concentração de AG3 promoveu elevação significativa na altura média e máxima das brotações e aumento no tamanho de calos. Nos dois experimentos, observou-se maior número de brotações na cultivar Amarela Comum.Two experiments were carried out to evaluate the effect of BAP and GA3 concentrations on the in vitro arracacha development. The concentrations tested were 0.0; 0.2 and 0.4 mg L-1 of BAP and 0.0; 0.125 and 0.250 mg L-1 of GA3 in the shoot tips with about 2 mm of arracacha, cultivars Amarela de Senador Amaral and Amarela Comum. The interaction of cultivars x BAP concentration was not significant for any one of the evaluated variables (P>0,0617. The increase in the BAP concentration reduced the callus formation; however, promoted larger reduction in the shoot development. The best BAP concentration was around 0.3 mg L-1, with relatively reduced callus diameter and good development of shoot, with a mean of 4.5 shoots

  4. Callus cell proliferation from broccoli leaf slice using IBA and BAP in vitro culture: Its biochemical and antioxidant properties.

    Science.gov (United States)

    Sharif Hossain, A B M; Haq, Imdadul; Ibrahim, Nasir A; Aleissa, Mohammed Saad

    2016-03-01

    Plant tissue or cell culture keeps a significant role in micro-propagation in the plant production industry. Combination of 6-Benzylaminopurine (BAP) and other plant growth regulators like 1-Naphthaleneacetic acid (NAA) or Indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) was used in the most of the research in tissue culture. The study was carried out to investigate the optimization of the concentration of IBA and BAP combination (0, 0.25, 0.50, 1.0, 1.50, 2.0, 2.5, 3.0 and 3.5 mg/l) for the root, callus and leaf proliferation from the leaf cutting slice. The highest number (6.75) of root proliferation was observed in the concentration of 2.0 mg/l IBA+0.25 mg/l BAP combination. The callus initiation was found in the concentration of IBA 1.0-3.5 mg/l+BAP 1.0-2.0 mg/l. However, the highest callus weight was observed at the concentration of IBA 1.5 mg/l+BAP 1.0 mg/l combination than other combination of concentrations. Positively leaf initiation and formation was better in the concentration of IBA 1-3.5 mg/l+BAP 1.0-2.0 mg/l combination. In addition, the 2,2-diphenyl-2-picrylhydarzyl (DPPH) free radical scavenging potential was higher (70.1%) in leaves extract than in callus extracts (46.3%) at the concentration of 10 mg/ml though both extracts had lower DPPH free radical scavenging activity compared to the positive control, vitamin C and BHT. Theresults conclude that the optimum concentration was IBA 1.5 mg/l+BAP 1.0 mg/l combination to produce callus cell proliferation and concentration of 2.0 mg/l IBA+0.25 mg/l BAP combination was the optimum for root proliferation of broccoli in vitro.

  5. Callus cell proliferation from broccoli leaf slice using IBA and BAP in vitro culture: Its biochemical and antioxidant properties

    Directory of Open Access Journals (Sweden)

    A.B.M. Sharif Hossain

    2016-03-01

    Full Text Available Plant tissue or cell culture keeps a significant role in micro-propagation in the plant production industry. Combination of 6-Benzylaminopurine (BAP and other plant growth regulators like 1-Naphthaleneacetic acid (NAA or Indole-3-acetic acid (IAA or indole-3-butyric acid (IBA was used in the most of the research in tissue culture. The study was carried out to investigate the optimization of the concentration of IBA and BAP combination (0, 0.25, 0.50, 1.0, 1.50, 2.0, 2.5, 3.0 and 3.5 mg/l for the root, callus and leaf proliferation from the leaf cutting slice. The highest number (6.75 of root proliferation was observed in the concentration of 2.0 mg/l IBA+0.25 mg/l BAP combination. The callus initiation was found in the concentration of IBA 1.0–3.5 mg/l+BAP 1.0–2.0 mg/l. However, the highest callus weight was observed at the concentration of IBA 1.5 mg/l+BAP 1.0 mg/l combination than other combination of concentrations. Positively leaf initiation and formation was better in the concentration of IBA 1–3.5 mg/l+BAP 1.0–2.0 mg/l combination. In addition, the 2,2-diphenyl-2-picrylhydarzyl (DPPH free radical scavenging potential was higher (70.1% in leaves extract than in callus extracts (46.3% at the concentration of 10 mg/ml though both extracts had lower DPPH free radical scavenging activity compared to the positive control, vitamin C and BHT. Theresults conclude that the optimum concentration was IBA 1.5 mg/l+BAP 1.0 mg/l combination to produce callus cell proliferation and concentration of 2.0 mg/l IBA+0.25 mg/l BAP combination was the optimum for root proliferation of broccoli in vitro.

  6. Pertumbuhan Eksplan Tunas Apikal Kelapa Sawit (ElaeisguineensisJacq.) Pada Media Ms Dengan Kombinasi BAP dan 2,4-D

    OpenAIRE

    Astuti, Novi

    2015-01-01

    The research about oil palm (Elaeis guineensis Jacq.) apical bud in the MS medium by using combination 2,4-D and BAP has been performed in Tissue Culture Laboratory, Departemen of Biology, University of Sumatera Utara.The aim of this research was to determine the effect of concentration of Benzyl Amino Purin (BAP) and 2,4- dichlorophenoxy acetic acid on the growth of the apical bud oil palm in vitro culture. Completely Randomized Design Method (RAL) with 2 factors namely 2,4-D with fourlevels...

  7. Immunological evaluation of OMV(PagL)+Bap(1-487aa) and AbOmpA(8-346aa)+Bap(1-487aa) as vaccine candidates against Acinetobacter baumannii sepsis infection.

    Science.gov (United States)

    Badmasti, Farzad; Ajdary, Soheila; Bouzari, Saeid; Fooladi, Abbas Ali Imani; Shahcheraghi, Fereshteh; Siadat, Seyed Davar

    2015-10-01

    Acinetobacter baumannii is an important nosocomial pathogen that causes a high morbidity and mortality rate in infected patients with sepsis form. The surface exposed virulence proteins and serum resistance factors helping to dissemination of this bacterium to bloodstream are the most promising vaccine candidates against this microorganism. In this project we immunologically evaluated OMV(PagL)+Bap(1-487aa) and AbOmpA (8-346aa)+Bap(1-487aa) as combination forms as well as Bap(1-487aa), AbOmpA(8-346aa) and OMV(PagL) singly, with addition of alum adjuvant as vaccine candidates. The titers of total IgG, IgG1 and IgG2c as well as concentration of IL-4 and IFN-γ and survival rates were measured in a C57BL/6 murine model with disseminated sepsis. The ratio of IgG1/IgG2c and profile of IL-4/IFN-γ in OMV (PagL)+Bap (1-487aa) formulation shows the humoral and cellular immune responses have been induced robustly and have created a full protection against A. baumannii ATCC 19606 and MDR AB-44 strains. We found that the two combination vaccine candidates were protective and induced both Th1 and Th2 responses.

  8. Ozone pollution affects flower numbers and timing in a simulated BAP priority calcareous grassland community.

    Science.gov (United States)

    Hayes, Felicity; Williamson, Jennifer; Mills, Gina

    2012-04-01

    Mesocosms representing the BAP Priority habitat 'Calcareous Grassland' were exposed to eight ozone profiles for twelve-weeks in two consecutive years. Half of the mesocosms received a reduced watering regime during the exposure periods. Numbers and timing of flowering in the second exposure period were related to ozone concentration and phytotoxic ozone dose (accumulated stomatal flux). For Lotus corniculatus, ozone accelerated the timing of the maximum number of flowers. An increase in mean ozone concentration from 30ppb to 70ppb corresponded with an advance in the timing of maximum flowering by six days. A significant reduction in flower numbers with increasing ozone was found for Campanula rotundifolia and Scabiosa columbaria and the relationship with ozone was stronger for those that were well-watered than for those with reduced watering. These changes in flowering timing and numbers could have large ecological impacts, affecting plant pollination and the food supply of nectar feeding insects.

  9. Genetic evolution of nevus of Ota reveals clonal heterogeneity acquiring BAP1 and TP53 mutations.

    Science.gov (United States)

    Vivancos, Ana; Caratú, Ginevra; Matito, Judit; Muñoz, Eva; Ferrer, Berta; Hernández-Losa, Javier; Bodet, Domingo; Pérez-Alea, Mileidys; Cortés, Javier; Garcia-Patos, Vicente; Recio, Juan A

    2016-03-01

    Melanoma presents molecular alterations based on its anatomical location and exposure to environmental factors. Due to its intrinsic genetic heterogeneity, a simple snapshot of a tumor's genetic alterations does not reflect the tumor clonal complexity or specific gene-gene cooperation. Here, we studied the genetic alterations and clonal evolution of a unique patient with a Nevus of Ota that developed into a recurring uveal-like dermal melanoma. The Nevus of Ota and ulterior lesions contained GNAQ mutations were c-KIT positive, and tumors showed an increased RAS pathway activity during progression. Whole-exome sequencing of these lesions revealed the acquisition of BAP1 and TP53 mutations during tumor evolution, thereby unmasking clonal heterogeneity and allowing the identification of cooperating genes within the same tumor. Our results highlight the importance of studying tumor genetic evolution to identify cooperating mechanisms and delineate effective therapies.

  10. Promoting the use of BaP as a marker for PAH exposure in UK soils.

    Science.gov (United States)

    Bull, Sarah; Collins, Chris

    2013-02-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants that frequently accumulate in soils. There is therefore a requirement to determine their levels in contaminated environments for the purposes of determining impacts on human health. PAHs are a suite of individual chemicals, and there is an ongoing debate as to the most appropriate method for assessing the risk to humans from them. Two methods predominate: the surrogate marker approach and the toxic equivalency factor. The former assumes that all chemicals in a mixture have an equivalent toxicity. The toxic equivalency approach estimates the potency of individual chemicals relative to the usually most toxic Benzo(a)pyrene. The surrogate marker approach is believed to overestimate risk and the toxic equivalency factor to underestimate risk. When analysing the risks from soils, the surrogate marker approach is preferred due to its simplicity, but there are concerns because of the potential diversity of the PAH profile across the range of impacted soils. Using two independent data sets containing soils from 274 sites across a diverse range of locations, statistical analysis was undertaken to determine the differences in the composition of carcinogenic PAH between site locations, for example, rural versus industrial. Following principal components analysis, distinct population differences were not seen between site locations in spite of large differences in the total PAH burden between individual sites. Using all data, highly significant correlations were seen between BaP and other carcinogenic PAH with the majority of r (2) values > 0.8. Correlations with the European Food Standards Agency (EFSA) summed groups, that is, EFSA2, EFSA4 and EFSA8 had even higher correlations (r (2) > 0.95). We therefore conclude that BaP is a suitable surrogate marker to represent mixtures of PAH in soil during risk assessments.

  11. Computer program design specifications for the Balloon-borne Ultraviolet Stellar Spectrometer (BUSS) science data decommutation program (BAPS48)

    Science.gov (United States)

    Rodriguez, R. M.

    1975-01-01

    The Balloon-Borne Ultraviolet Stellar Spectrometer (BUSS) Science Data Docummutation Program (BAPS48) is a pulse code modulation docummutation program that will format the BUSS science data contained on a one inch PCM tracking tape into a seven track serial bit stream formatted digital tape.

  12. Persistence of Multidrug-Resistant Acinetobacter baumannii Isolates Harboring blaOXA-23 and bap for 5 Years.

    Science.gov (United States)

    Sung, Ji Youn; Koo, Sun Hoe; Kim, Semi; Kwon, Gye Cheol

    2016-08-28

    The emergence and dissemination of carbapenemase-producing Acinetobacter baumannii isolates have been reported worldwide, and A. baumannii isolates harboring blaOXA-23 are often resistant to various antimicrobial agents. Antimicrobial resistance can be particularly strong for biofilm-forming A. baumannii isolates. We investigated the genetic basis for carbapenem resistance and biofilm-forming ability of multidrug-resistant (MDR) clinical isolates. Ninety-two MDR A. baumannii isolates were collected from one university hospital located in the Chungcheong area of Korea over a 5-year period. Multiplex PCR and DNA sequencing were performed to characterize carbapenemase and bap genes. Clonal characteristics were analyzed using REP-PCR. In addition, imaging and quantification of biofilms were performed using a crystal violet assay. All 92 MDR A. baumannii isolates involved in our study contained the blaOXA-23 and bap genes. The average absorbance of biomass in Bap-producing strains was much greater than that in non-Bap-producing strains. In our study, only three REP-PCR types were found, and the isolates showing type A or type B were found more than 60 times among unique patients during the 5 years of surveillance. These results suggest that the isolates have persisted and colonized for 5 years, and biofilm formation ability has been responsible for their persistence and colonization.

  13. Farmacokinetiek en biologische beschikbaarheid van benzo(a)pyreen (BaP) in de RIV:tox rat

    NARCIS (Netherlands)

    Lusthof KJ; Olling M; Kroese ED; Beenen J; Poelen MJ; Vaessen HAMG; Kamp CG van de

    1993-01-01

    Eight groups of six male RIV:tox rats received oral and intravenous doses of benzo(a)pyrene (BaP) in a parallel study plan. Intravenously and orally four dose levels were administered. BaF was dissolved in soybean oil for oral administration, and in glycofurol for intravenous administration. The dos

  14. Biological apatite (BAp) crystallographic orientation and texture as a new index for assessing the microstructure and function of bone regenerated by tissue engineering.

    Science.gov (United States)

    Nakano, Takayoshi; Kaibara, Kazuhiro; Ishimoto, Takuya; Tabata, Yasuhiko; Umakoshi, Yukichi

    2012-10-01

    Recently, there have been remarkable advances in medical techniques for regenerating bone defects. To determine the degree of bone regeneration, it is essential to develop a new method that can analyze microstructure and related mechanical function. Here, quantitative analysis of the orientation distribution of biological apatite (BAp) crystallites by a microbeam X-ray diffractometer system is proposed as a new index of bone quality for the evaluation of regenerated bone microstructure. Preferential alignment of the BAp c-axis in the rabbit ulna and skull bone, regenerated by controlled release of basic fibroblast growth factor (bFGF) was investigated. The BAp c-axis orientation was evaluated by the relative intensity between the (002) and (310) diffraction peaks, or the three-dimensional texture for the (002) peak. It was found that new bone in the defects was initially produced without preferential alignment of the BAp c-axis, and subsequently reproduced to recover towards the original alignment. In other words, the BAp density recovered prior to the BAp orientation. Perfect recovery of BAp alignment was not achieved in the ulna and skull defects after 4 weeks and 12 weeks, respectively. Apparent recovery of the macroscopic shape and bio-mineralization of BAp was almost complete in the ulna defect after 4 weeks. However, an additional 2 weeks was required for complete repair of BAp orientation. It is finally concluded that orientation distribution of BAp crystallites offers an effective means of evaluating the degree of microstructural regeneration, and also the related mechanical function, in regenerated hard tissues.

  15. Evaluation of anti-cancer and anti-oxidative potential of Syzygium Cumini against benzo[a]pyrene (BaP) induced gastric carcinogenesis in mice.

    Science.gov (United States)

    Goyal, P K; Verma, Preeti; Sharma, Priyanka; Parmar, Jyoti; Agarwal, Annapurna

    2010-01-01

    Syzygium cummini extract (SCE) was used in the present study to explore anti-tumor promoting activity in a stomach carcinogenesis model in mice. For this purpose, Swiss albino mice were administered with 1 mg of benzo-a-pyrene (BaP) in 100?l sesame oil by oral gavage twice a week for 4 consecutive weeks. The animals were sacrificed 14 weeks after the last administration of BaP. Oral administration of the extract to pre-treated (i.e. SCE as 25mg/kg b. wt./ day before BaP application for 2 weeks), post-treated (i.e. SCE after BaP application for 8 weeks) and pre-post treated (i.e. SCE for 2 weeks before treatment of BaP followed by the concomitant treatment with SCE and BaP for 4 weeks during and 2 weeks after the last dose of BaP) groups provided a significant reduction in tumor incidence, tumor burden and cumulative number of gastric carcinomas along with a significant elevation of phase II detoxifying enzymes, and inhibition of lipid per oxidation in the stomach. Thus, the present data suggest that the Syzygium cummini extract has anti-tumor and anti-oxidative potential against chemical induced stomach carcinogenesis.

  16. Toxic effects of benzo[a]pyrene (Bap) and Aroclor1254 on embryogenesis, larval growth, survival and metamorphosis of the bivalve Meretrix meretrix.

    Science.gov (United States)

    Wang, Qing; Yang, Hongsheng; Liu, Baozhong; Wang, Xiaoyu

    2012-08-01

    To assess the potential toxicity of polycyclic aromatic hydrocarbons and polychlorinated biphenyls on the early development of Meretrix meretrix, the effects of benzo[a]pyrene (Bap) and Aroclor1254 on embryogenesis and larval development were investigated using static laboratory toxicity tests at nominal concentrations of 6.25-1,600 μg/L. Even at 1,600 μg/L, Bap and Aroclor1254 only caused minor reductions in embryo development rates. The 96 h LC(50) values for D-shaped larvae were 156 μg/L for Bap and 132 μg/L for Aroclor1254, respectively. The most sensitive toxicity endpoint in this study was metamorphosis, with an EC(50) value of 20 μg/L for Bap and 35 μg/L for Aroclor1254. Aroclor1254 was more toxic than Bap to embryos and larvae. Our results indicate that Bap and Aroclor1254 do not show extreme toxicity to M. meretrix embryos and larvae. These data provide information for evaluating the toxicity of Bap and Aroclor1254 on bivalve embryos, especially over the entire larval stages.

  17. BAP, a mammalian BiP-associated protein, is a nucleotide exchange factor that regulates the ATPase activity of BiP.

    Science.gov (United States)

    Chung, Kyung Tae; Shen, Ying; Hendershot, Linda M

    2002-12-01

    We identified a mammalian BiP-associated protein, BAP, using a yeast two-hybrid screen that shared low homology with yeast Sls1p/Sil1p and mammalian HspBP1, both of which regulate the ATPase activity of their Hsp70 partner. BAP encoded an approximately 54-kDa protein with an N-terminal endoplasmic reticulum (ER) targeting sequence, two sites of N-linked glycosylation, and a C-terminal ER retention sequence. Immunofluorescence staining demonstrated that BAP co-localized with GRP94 in the endoplasmic reticulum. BAP was ubiquitously expressed but showed the highest levels of expression in secretory organ tissues, a pattern similar to that observed with BiP. BAP binding was affected by the conformation of the ATPase domain of BiP based on in vivo binding studies with BiP mutants. BAP stimulated the ATPase activity of BiP when added alone or together with the ER DnaJ protein, ERdj4, by promoting the release of ADP from BiP. Together, these data demonstrate that BAP serves as a nucleotide exchange factor for BiP and provide insights into the mechanisms that control protein folding in the mammalian ER.

  18. Evaluation of anti-cancer and anti-oxidative potential of Syzygium Cumini against benzo[a]pyrene (BaP) induced gastric carcinogenesis in mice.

    Science.gov (United States)

    Goyal, P K; Verma, Preeti; Sharma, Priyanka; Parmar, Jyoti; Agarwal, Annapurna

    2010-01-01

    Syzygium cummini extract (SCE) was used in the present study to explore anti-tumor promoting activity in a stomach carcinogenesis model in mice. For this purpose, Swiss albino mice were administered with 1 mg of benzo-a-pyrene (BaP) in 100?l sesame oil by oral gavage twice a week for 4 consecutive weeks. The animals were sacrificed 14 weeks after the last administration of BaP. Oral administration of the extract to pre-treated (i.e. SCE as 25mg/kg b. wt./ day before BaP application for 2 weeks), post-treated (i.e. SCE after BaP application for 8 weeks) and pre-post treated (i.e. SCE for 2 weeks before treatment of BaP followed by the concomitant treatment with SCE and BaP for 4 weeks during and 2 weeks after the last dose of BaP) groups provided a significant reduction in tumor incidence, tumor burden and cumulative number of gastric carcinomas along with a significant elevation of phase II detoxifying enzymes, and inhibition of lipid per oxidation in the stomach. Thus, the present data suggest that the Syzygium cummini extract has anti-tumor and anti-oxidative potential against chemical induced stomach carcinogenesis. PMID:21039048

  19. Inflammatory effects of BaP1 a metalloproteinase isolated from Bothrops asper snake venom: leukocyte recruitment and release of cytokines.

    Science.gov (United States)

    Fernandes, Cristina Maria; Zamuner, Stella Regina; Zuliani, Juliana Pavan; Rucavado, Alexandra; Gutiérrez, José Maria; Teixeira, Catarina de Fátima Pereira

    2006-04-01

    The inflammatory events induced by BaP1, a 22.7 kDa metalloproteinase isolated from Bothrops asper snake venom, were studied. BaP1 i.p. injection in mice induced a marked inflammatory cell infiltrate into peritoneal cavity of animals with predominance of neutrophils in the early phase followed by mononuclear cells in the late period. Inhibition of enzymatic activity of BaP1 by chelation with EDTA resulted in a drastic reduction of this effect. In addition, BaP1 induced a significant increase of blood neutrophil numbers before its accumulation in peritoneal cavity, thus suggesting a stimulatory action of BaP1 on mechanisms of cell mobilization from bone marrow reserve compartments. A reduction in the number of neutrophils was observed in the exudate when antibodies against LECAM-1, CD18 and LFA-1 were used, suggesting the involvement of these adhesion molecules in the effects of BaP1. In contrast, there was no effect with antibodies against ICAM-1 and PECAM-1. Moreover, a conspicuous increment in the levels of IL-1 and TNF-alpha, but not of LTB4, was observed in peritoneal washes collected from mice injected with BaP1. It is concluded that BaP1 induces in vivo a marked leukocyte influx, which parallels an increased number of these cells in the blood, and is associated to the expression of specific leukocyte adhesion molecules and release of chemotactic inflammatory cytokines. Since BaP1 is a P-I class metalloproteinase, these results indicate that the proteolytic domain of metalloproteinases per se can trigger specific inflammatory events. PMID:16529786

  20. BAP Position Statement: Off-label prescribing of psychotropic medication to children and adolescents.

    Science.gov (United States)

    Sharma, Aditya N; Arango, Celso; Coghill, David; Gringras, Paul; Nutt, David J; Pratt, Peter; Young, Allan H; Hollis, Chris

    2016-05-01

    The off-label use of medicines for children and adolescents remains a common and important issue for prescribing practice across child and adolescent psychiatry, paediatrics and primary care. This editorial focusses on psychotropic drug treatment, which plays an essential part in the comprehensive management of a range of child and adolescent psychiatric disorders. Despite a growing evidence base for drug treatment in child and adolescent psychiatric disorders, much psychotropic medication continues to be prescribed off-label (i.e. outside the limits of the marketing authorisation or product license). The reasons for and implications of off-label prescribing, including the potential clinical benefits/risks and medico-legal implications, are often poorly understood by both patients and prescribers. An important unintended consequence of the uncertainties and confusion surrounding the status of off-label prescribing for children and adolescents may be that effective drug treatments are being withheld or underused. This BAP Position Statement aims to clarify these issues, challenge some of the myths surrounding off-label prescribing for children and adolescents and offer practical guidance for prescribers. PMID:27098018

  1. BAP Position Statement: Off-label prescribing of psychotropic medication to children and adolescents.

    Science.gov (United States)

    Sharma, Aditya N; Arango, Celso; Coghill, David; Gringras, Paul; Nutt, David J; Pratt, Peter; Young, Allan H; Hollis, Chris

    2016-05-01

    The off-label use of medicines for children and adolescents remains a common and important issue for prescribing practice across child and adolescent psychiatry, paediatrics and primary care. This editorial focusses on psychotropic drug treatment, which plays an essential part in the comprehensive management of a range of child and adolescent psychiatric disorders. Despite a growing evidence base for drug treatment in child and adolescent psychiatric disorders, much psychotropic medication continues to be prescribed off-label (i.e. outside the limits of the marketing authorisation or product license). The reasons for and implications of off-label prescribing, including the potential clinical benefits/risks and medico-legal implications, are often poorly understood by both patients and prescribers. An important unintended consequence of the uncertainties and confusion surrounding the status of off-label prescribing for children and adolescents may be that effective drug treatments are being withheld or underused. This BAP Position Statement aims to clarify these issues, challenge some of the myths surrounding off-label prescribing for children and adolescents and offer practical guidance for prescribers.

  2. Determination of respiration, gross nitrification and denitrification in soil profile using BaPS system

    Institute of Scientific and Technical Information of China (English)

    CHEN Shu-tao; HUANG Yao

    2006-01-01

    A facility of BaPS (Barometric Process Separation) was used to determine soil respiration, gross nitrification and denitrification in a winter wheat field with depths of 0-7, 7-14 and 14-21 cm. N2O production was determined by a gas chromatograph. Crop root mass and relevant soil parameters were measured. Results showed that soil respiration and gross nitrification decreased with the increase of soil depth, while denitrification did not change significantly. In comparison with no-plowing plot, soil respiration increased significantly in plowing plot, especially in the surface soil of 0-7 cm, while gross nitrification and denitrification rates were not affected by plowing. Cropping practice in previous season was found to affect soil gross nitrification in the following wheat-growing season. Higher gross nitrification rate occurred in the filed plot with preceding crop of rice compared with that of maize for all the three depths of 0-7, 7-14 and 14-21 cm. A further investigation indicated that the nitrification for all the cases accounted for about 76% of the total nitrogen transformation processes of nitrification and denitrification and the N2O production correlated with nitrification significantly, suggesting that nitrification is a key process of soil N2O production in the wheat field. In addition, the variations of soil respiration and gross nitrification were exponentially dependent on root mass (P<0.001).

  3. The stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests on stored horse blood.

    Science.gov (United States)

    Celi, P; Sullivan, M; Evans, D

    2010-02-01

    Increasing interest in the role of oxidative stress (OS) in equine medicine has highlighted the need to develop reliable methods to quantify it. In this study we describe the effect of refrigeration (at 4 degrees C) on the stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests carried out on 15 healthy horses. Blood samples, collected from the jugular vein, were immediately placed on ice and analysed using both the d-ROMs and BAP tests. Samples were also refrigerated at 4 degrees C and tested after 3, 7 and 24 h. The average results were similar for up to 24 h and minimal variations were found for each horse. The findings suggest that refrigeration is suitable for preserving equine blood samples for these assays and this approach will provide veterinarians with a technically simple, reliable test to measure OS under field conditions.

  4. Somatic embryo-like structures of strawberry regenerated in vitro on media supplemented with 2,4-D and BAP.

    Science.gov (United States)

    Omar, Genesia F; Mohamed, Fouad H; Haensch, Klaus-Thomas; Sarg, Sawsan H; Morsey, Mohamed M

    2013-09-01

    Somatic embryo-like structures (SELS) were produced in vitro from leaf disk and petiole explants of two cultivars of strawberry (Fragaria x ananassa Duch) on Murashige and Skoog medium with different concentrations and combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and sucrose to check the embryonic nature of these structures histologically. A large number of SELS could be regenerated in both cultivars on media with 2-4 mg L(-1) 2,4-D in combination with 0.5 -1 mg L(-1) BAP and 50 g x L(-1) sucrose. Histological examination of SELS revealed the absence of a root pole. Therefore these structures cannot be strictly classified as somatic embryos. The SELS formed under the tested culture conditions represent malformed shoot-like and leaf-like structures. The importance of these results for the propagation of strawberries via somatic embryogenesis is discussed.

  5. Somatic embryo-like structures of strawberry regenerated in vitro on media supplemented with 2,4-D and BAP.

    Science.gov (United States)

    Omar, Genesia F; Mohamed, Fouad H; Haensch, Klaus-Thomas; Sarg, Sawsan H; Morsey, Mohamed M

    2013-09-01

    Somatic embryo-like structures (SELS) were produced in vitro from leaf disk and petiole explants of two cultivars of strawberry (Fragaria x ananassa Duch) on Murashige and Skoog medium with different concentrations and combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and sucrose to check the embryonic nature of these structures histologically. A large number of SELS could be regenerated in both cultivars on media with 2-4 mg L(-1) 2,4-D in combination with 0.5 -1 mg L(-1) BAP and 50 g x L(-1) sucrose. Histological examination of SELS revealed the absence of a root pole. Therefore these structures cannot be strictly classified as somatic embryos. The SELS formed under the tested culture conditions represent malformed shoot-like and leaf-like structures. The importance of these results for the propagation of strawberries via somatic embryogenesis is discussed. PMID:24377134

  6. The no-observed-adverse-effect level (NOAEL) of baby aloe powder (BAP) for nutraceutical application based upon toxicological evaluation.

    Science.gov (United States)

    Kwack, Seung Jun; Do, Seon-Gil; Kim, Young Woo; Kim, Yeon-Joo; Gwak, Hyo-Min; Park, Hyun Jong; Roh, Taehyun; Shin, Min Kyung; Lim, Seong Kwang; Kim, Hyung Sik; Lee, Byung-Mu

    2014-01-01

    Aloe has been used in versatile herbal medications and nutraceuticals throughout history. Aloe is widely considered to be generally safe for humans and used globally. The effectiveness and pharmacological properties of aloe are dependent upon when the plant is collected. However, little is known about the toxicology of whole-body aloe collected within less than 1 yr. Based upon widespread exposure to aloe, it is important to determine a daily intake level of this chemical to ensure its safety for humans. To determine the no-observed-adverse-effect level (NOAEL) of baby aloe powder (BAP) for clinical application, Sprague-Dawley (SD) rats were treated orally for 4 wk with 4 different concentrations: 0, 0.125, 0.5, and 2 g/kg body weight (bw). In this study, no significant or dose-dependent toxicological effects of BAP were observed in biochemical or hematological parameters, urinalysis, clinical signs, body weight, and food and water consumption. There were changes in some biomarkers in certain treated groups compared to controls; however, all values were within their reference ranges and not dose-dependent. Based on these results, the NOAEL of BAP was estimated to be greater than 2 g/kg bw in male and 2 g/kg bw in female SD rats. Collectively, these data suggest that BAP used in this study did not produce any marked subacute toxic effects up to a maximum concentration of 2 g/kg bw, and thus use in nutraceuticals and in pharmaceutical and cosmetic applications at a concentration of >2 g/kg is warranted.

  7. 无线局域网安全协议BAP研究%Research of Wireless LAN Security Protocol BAP

    Institute of Scientific and Technical Information of China (English)

    姚会娟; 周祥

    2016-01-01

    随着无线局域网的普及,它的安全性备受人们的关注,文章就此问题提出了一种新的安全协议BA P。%With the popularity of wireless LAN, its security has attracted people's attention. In this paper, we propose a new security prot ocol BAP.

  8. The Study of Inhibition Effects Satureja khuzestaniea Essence against Gene Expression bap Acinetobacter baumannii with Real time PCR Technique

    OpenAIRE

    Abbas Bahador; Hossein Saghii; Ramezal Ataee; Davoud Esmaeili

    2015-01-01

    Background and Aim: Acinetobacter baumannii a major hospital pathogens and causes outbreaks of infections and associated to nosocomial infections, including bacteremia, pneumonia, meningitis, urinary tract infection, and wound infections. Satureja khuzestaniea province the group Nnayyan and Carvacrol and thymol are antimicrobial activity of this plant. Biofilm-related genes (bap)-specific proteins on the cell surface generate a direct role in biofilm formation and infection of the bacteria is...

  9. MACROPROPAGATION OF PLANTAIN (MUSA SPP. CULTIVARS PITA 3, FHIA 21, ORISHELE AND CORNE 1: EFFECT OF BENZYLAMINOPURINE (BAP CONCENTRATION

    Directory of Open Access Journals (Sweden)

    Deless Edmond Fulgence THIEMELE

    2015-12-01

    Full Text Available In Africa, plantain is one of the most important starchy food and cash crops. Nonetheless, one of the major constraints for its production was the unavailability of healthy planting materials at planting time. This constraint could be lifted using the cloning of planting materials via the in vitro micropropagation or in vivo macropropagation techniques. Shelled corms from four cultivars, known as PITA 3, FHIA 21, ORISHELE and CORNE 1, were used. Three treatments differing in three hormonal concentrations, especially 20.0, 30.0 and 40.0 mg L-1 were tested. The control one was hormone free. Tested treatments were laid out in a split plot design. The decorticated banana corms were sprayed twofold at 2 weeks interval with BAP solution when placed in sterilized soil in high humidity plastic tunnel. It emerged from results, regarding BAP concentration effect, that BAP treatment with 40 mg L-1 significantly reduced the emergence time of shoots at 20 days as against 25.1, 28.3 and 28.5 for the 2 tested other treatments as well as control, respectively. Likewise, the concentrations 40.0 mg L-1 both recorded the largest number of sprouted buds per corm and number of shoots per corm. With respect to banana cultivar effect, PITA 3 showed the largest number of shoots per corm. Basing on such findings, it is concluded that MSD technique combined with BAP at 40.0 mg L-1 is a suitable technique for improving of the in vivo macropropagation of plantain. This concentration increased at least 50 % of sucker production compared to control.

  10. Pertumbuhan Eksplan Bunga Betina Kelapa Sawit (Elaeis guineensis Jacq.) Pada Media MS Dengan Kombinasi 2,4D Dan BAP

    OpenAIRE

    Nasution, Siti Shofiya

    2015-01-01

    Penelitian tentang “Pertumbuhan Eksplan Bunga Betina Kelapa Sawit (Elaeis guineensis Jacq.) pada Media MS dengan Kombinasi 2,4-D dan BAP” telah dilakukan di Laboratorium Kultur Jaringan Tumbuhan Departemen Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara dari bulan Januari 2013 sampai dengan bulan Desember 2013. Penelitian yang bertujuan untuk mengetahui kombinasi 2,4-D dan BAP terbaik pada induksi kalus dan pertumbuhan kultur eksplan bunga betina kelapa sawit...

  11. Hepcidin gene expression induced in the developmental stages of fish upon exposure to Benzo[a]pyrene (BaP).

    Science.gov (United States)

    Wang, Ke-Jian; Bo, Jun; Yang, Ming; Hong, Hua-Sheng; Wang, Xin-Hong; Chen, Fang-Yi; Yuan, Jian-Jun

    2009-04-01

    Hepcidin is known to be expressed in fish with bacterial challenge and iron overload. Here we first report the hepcidin expression induced in the developmental stages from embryo to fry of red sea bream (Pagarus major) and in juvenile black porgy (Acanthopagrus schlegelii B.) upon continuous waterborne exposure to BaP. The gene expression of CYP1A1 and IgL (immunoglobulin light chain) were both measured. Expression of the Pagarus major hepcidin gene (PM-hepc) was increased in post hatch fry at 24 h and 120 h exposure to BaP at concentrations of 0.1, 0.5 and 1.0 microg/l, respectively. The gene expression pattern was comparable to that of CYP1A1 but different from that of IgL. In addition, a high number of AS-hepc2 transcripts (Acanthopagrus schlegelii B. hepcidin gene) were detected in the liver upon exposure to 1.0 microg/l BaP. This study demonstrates that hepcidin gene expression is significantly induced in BaP-exposed red sea bream and black porgy.

  12. Serum BAP as the clinically useful marker for predicting BMD reduction in diabetic hemodialysis patients with low PTH.

    Science.gov (United States)

    Ueda, Misako; Inaba, Masaaki; Okuno, Senji; Maeno, Yoshifumi; Ishimura, Eiji; Yamakawa, Tomoyuki; Nishizawa, Yoshiki

    2005-07-22

    With decrease of serum PTH in hemodialysis (HD) patients, other factors besides parathyroid hormone (PTH) become important in regulating bone metabolism. We investigated which serum bone metabolic marker is the best to predict the bone mineral density (BMD) reduction in HD patients with serum PTHBAP), intact osteocalcin (OC), and N-terminal propeptide of type I collagen (PINP), and the bone resorption markers, deoxypyridinoline (DPD), pyridinoline (PYD), and beta-crossLaps (beta-CTx) were measured in serum from 137 HD patients. BMD of all patients was measured twice, approximately 1.5 years before and 1.5 years after measurement of their markers of bone metabolism. In all 137 HD patients, serum BAP was the only marker significantly higher in those with BMD reduction than in those without. In 42 diabetes mellitus (DM) HD patients with serum PTHBAP was again the only marker to discriminate those with BMD reduction from those without. At serum PTHBAP retained tendency toward higher value. These findings suggest that serum BAP might be the most sensitive to identify small changes of bone metabolism in low bone turnover state. Retrospective study confirmed the usefulness of serum BAP in clinical practice by significantly higher values in those with bone loss at PTHBAP is a clinically useful bone formation marker to predict the BMD reduction in DM HD patients with low level of PTH.

  13. Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(aP and an heterocyclic amine (PhIP in relation to colorectal carcinogenesis.

    Directory of Open Access Journals (Sweden)

    Emilien L Jamin

    Full Text Available Colorectal neoplasia is the third most common cancer worldwide. Environmental factors such as diet are known to be involved in the etiology of this cancer. Several epidemiological studies have suggested that specific neo-formed mutagenic compounds related to meat consumption are an underlying factor involved in the association between diet and colorectal cancer. Heterocyclic amines (HCAs and polycyclic aromatic hydrocarbons (PAHs are known mutagens and possible human carcinogens formed at the same time in meat during cooking processes. We studied the genotoxicity of the model PAH benzo(apyrene (B(aP and HCA 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP, alone or in mixture, using the mouse intestinal cell line Apc(Min/+, mimicking the early step of colorectal carcinogenesis, and control Apc(+/+ cells. The genotoxicity of B(aP and PhIP was investigated using both cell lines, through the quantification of B(aP and PhIP derived DNA adducts, as well as the use of a genotoxic assay based on histone H2AX phosphorylation quantification. Our results demonstrate that heterozygous Apc mutated cells are more effective to metabolize B(aP. We also established in different experiments that PhIP and B(aP were more genotoxic on Apc (Min/+ cells compared to Apc (+/+ . Moreover when tested in mixture, we observed a combined genotoxicity of B(aP and PhIP on the two cell lines, with an increase of PhIP derived DNA adducts in the presence of B(aP. Because of their genotoxic effects observed on heterozygous Apc mutated cells and their possible combined genotoxic effects, both B(aP and PhIP, taken together, could be implicated in the observed association between meat consumption and colorectal cancer.

  14. Strategies to develop market access that contribute to resilience in the Bolivian highlands: Case study: PMCA and BAP for chuño and tunta

    OpenAIRE

    Figueroa, M; Valdivia, Corinne

    2008-01-01

    The study evaluates how the Bolivian Andean Platform (BAP), under the philosophy of the Participatory Market Chain Approach (PMCA) reduces transaction costs for native products, Chuno and Tunta, elaborated by small-scale farmers in three communities in Umala-Bolivia. At a first stage, the study identifies how local native potato varieties' programs (NPVP) developed by the International Potato Center (CIP)-ALTAGRO development project empower farmers to be able to participate in the BAP. It als...

  15. Identification of bapA in Strains of Salmonella enterica subsp. enterica Isolated from Wild Animals Kept in Captivity in Sinaloa, Mexico.

    Science.gov (United States)

    Silva-Hidalgo, Gabriela; López-Valenzuela, Martin; Cárcamo-Aréchiga, Nora; Cota-Guajardo, Silvia; López-Salazar, Mayra; Montiel-Vázquez, Edith

    2016-01-01

    bapA, previously named stm2689, encodes the BapA protein, which, along with cellulose and fimbriae, constitutes biofilms. Biofilms are communities of microorganisms that grow in a matrix of exopolysaccharides and may adhere to living tissues or inert surfaces. Biofilm formation is associated with the ability to persist in different environments, which contributes to the pathogenicity of several species. We analyzed the presence of bapA in 83 strains belonging to 17 serovars of Salmonella enterica subsp. enterica from wildlife in captivity at Culiacan's Zoo and Mazatlán's Aquarium. Each isolate amplified a product of 667 bp, which corresponds to the expected size of the bapA initiator, with no observed variation between different serovars analyzed. bapA gene was found to be highly conserved in Salmonella and can be targeted for the genus-specific detection of this organism from different sources. Since bapA expression improves bacterial proliferation outside of the host and facilitates resistance to disinfectants and desiccation, the survival of Salmonella in natural habitats may be favored. Thus, the risk of bacterial contamination from these animals is increased. PMID:27379195

  16. Occurrence of genes coding for MSCRAMM and biofilm-associated protein Bap in Staphylococcus spp. isolated from bovine subclinical mastitis and relationship with somatic cell counts.

    Science.gov (United States)

    Zuniga, Eveline; Melville, Priscilla A; Saidenberg, André B S; Laes, Marco A; Gonsales, Fernanda F; Salaberry, Sandra R S; Gregori, Fabio; Brandão, Paulo E; dos Santos, Franklin G B; Lincopan, Nilton E; Benites, Nilson R

    2015-12-01

    This study aimed to elucidate aspects of the epidemiology of bovine subclinical mastitis through the assessment of genes encoding MSCRAMM (microbial surface components recognizing adhesive matrix molecules - a group of adhesins) and protein Bap (implicated in biofilm formation), in coagulase-positive (CPS) and coagulase-negative (CNS) Staphylococcus isolated from subclinical mastitis. Milk samples were collected for microbiological exams, somatic cell count (SCC) and a survey of the genes coding for MSCRAMM (cna, eno, ebpS, fnbA, fnbB and fib) and biofilm-associated protein Bap (bap) in 106 Staphylococcus spp. isolates using PCR. The frequencies of occurrence of eno (82.1%), fnbA (72.6%), fib (71.7%) and bap (56.6%) were higher (P bap gene in CNS compared with CPS suggests that in these species biofilm formation is an important mechanism for the persistence of the infection. The medians of the SCCs in the samples where eno, fnbA, fib and bap genes were detected were higher compared with Staphylococcus without the assessed genes (P < 0.05) and negative samples (P < 0.01), which indicated that the presence of these MSCRAMM may be related to a higher intensity of the inflammatory process.

  17. Identification of bapA in Strains of Salmonella enterica subsp. enterica Isolated from Wild Animals Kept in Captivity in Sinaloa, Mexico

    Science.gov (United States)

    López-Valenzuela, Martin; Cárcamo-Aréchiga, Nora; Cota-Guajardo, Silvia; López-Salazar, Mayra; Montiel-Vázquez, Edith

    2016-01-01

    bapA, previously named stm2689, encodes the BapA protein, which, along with cellulose and fimbriae, constitutes biofilms. Biofilms are communities of microorganisms that grow in a matrix of exopolysaccharides and may adhere to living tissues or inert surfaces. Biofilm formation is associated with the ability to persist in different environments, which contributes to the pathogenicity of several species. We analyzed the presence of bapA in 83 strains belonging to 17 serovars of Salmonella enterica subsp. enterica from wildlife in captivity at Culiacan's Zoo and Mazatlán's Aquarium. Each isolate amplified a product of 667 bp, which corresponds to the expected size of the bapA initiator, with no observed variation between different serovars analyzed. bapA gene was found to be highly conserved in Salmonella and can be targeted for the genus-specific detection of this organism from different sources. Since bapA expression improves bacterial proliferation outside of the host and facilitates resistance to disinfectants and desiccation, the survival of Salmonella in natural habitats may be favored. Thus, the risk of bacterial contamination from these animals is increased. PMID:27379195

  18. Occurrence of genes coding for MSCRAMM and biofilm-associated protein Bap in Staphylococcus spp. isolated from bovine subclinical mastitis and relationship with somatic cell counts.

    Science.gov (United States)

    Zuniga, Eveline; Melville, Priscilla A; Saidenberg, André B S; Laes, Marco A; Gonsales, Fernanda F; Salaberry, Sandra R S; Gregori, Fabio; Brandão, Paulo E; dos Santos, Franklin G B; Lincopan, Nilton E; Benites, Nilson R

    2015-12-01

    This study aimed to elucidate aspects of the epidemiology of bovine subclinical mastitis through the assessment of genes encoding MSCRAMM (microbial surface components recognizing adhesive matrix molecules - a group of adhesins) and protein Bap (implicated in biofilm formation), in coagulase-positive (CPS) and coagulase-negative (CNS) Staphylococcus isolated from subclinical mastitis. Milk samples were collected for microbiological exams, somatic cell count (SCC) and a survey of the genes coding for MSCRAMM (cna, eno, ebpS, fnbA, fnbB and fib) and biofilm-associated protein Bap (bap) in 106 Staphylococcus spp. isolates using PCR. The frequencies of occurrence of eno (82.1%), fnbA (72.6%), fib (71.7%) and bap (56.6%) were higher (P < 0.0001) compared with the other assessed genes (cna, ebpS and fnbB). The higher frequency of occurrence (P < 0.005) of the bap gene in CNS compared with CPS suggests that in these species biofilm formation is an important mechanism for the persistence of the infection. The medians of the SCCs in the samples where eno, fnbA, fib and bap genes were detected were higher compared with Staphylococcus without the assessed genes (P < 0.05) and negative samples (P < 0.01), which indicated that the presence of these MSCRAMM may be related to a higher intensity of the inflammatory process. PMID:26318876

  19. The combined effects of BDE47 and BaP on oxidatively generated DNA damage in L02 cells and the possible molecular mechanism.

    Science.gov (United States)

    An, Jing; Yin, Lingling; Shang, Yu; Zhong, Yufang; Zhang, Xinyu; Wu, Minghong; Yu, Zhiqiang; Sheng, Guoying; Fu, Jiamo; Huang, Yuecheng

    2011-04-01

    Polybrominated diphenyl ethers (PBDEs) and polycyclic aromatic hydrocarbons (PAHs) coexist widely in the environment and have generated adverse effects on the environment and human health. The purpose of this study was to investigate the combined toxic effects of these chemicals and the related mechanism. L02 cells were exposed to BDE47 (5, 10μmol/L) or/and BaP (50μmol/L) in different administration order. The cell growth and survival, DNA strand breaks, oxidative stress index (ROS, SOD, GSH, and MDA), LDH release and the expression level of CYP1 family members were measured. The result showed that BDE47 or/and BaP had no effect on the cell growth and survival under the present conditions. However, compared with the groups treated with BDE47 or BaP alone, the combined-treated groups induced significantly elevated DNA strand breaks, ROS production, and MDA level. Especially, pretreatment with BDE47 followed by BaP led to the strongest effects. Addition of the antioxidant N-acetyl-l-cysteine (NAC) markedly reduced the ROS level and partly suppressed the DNA strand breaks induced by BDE47 or/and BaP. Meanwhile, the combined treatment groups also markedly increased the SOD activity, GSH content, and LDH release level compared with the control group. The real-time PCR results showed that BaP could significantly induce the expression of CYP1A1 and CYP1B1, however, the pre-treatment with BDE47 appeared to attenuate the BaP-induced CYP1 expression. All of above findings indicated that BDE47 and BaP had a synergistic effect on oxidatively generated DNA damage in L02 cells via regulation on the oxidative stress response and the expression of CYP1 metabolism enzymes.

  20. The Japanese medaka (Oryzias latipes) model: applicability for investigating the immunosuppressive effects of the aquatic pollutant benzo[a]pyrene (BaP).

    Science.gov (United States)

    Carlson, E A; Li, Y; Zelikoff, J T

    2002-01-01

    Despite the fact that BaP is a carcinogen, mammalian immunosuppressant, and ubiquitous aquatic pollutant, knowledge regarding the effects of BaP on the immune system of fish is still lacking. To begin to fill this gap, studies were conducted in medaka to examine the effects and mechanisms by which BaP exposure might alter host immunocompetence. Fish, exposed by IP injection of BaP (2-600 microg/g BW), were examined after 48 h for effects upon immune function and CYP1A expression/activity. Benzo[a]pyrene, at a concentration below that which increased levels of CYPIA expression/activity (2 microg BaP/g BW) suppressed lymphocyte proliferation. Concentrations of BaP at 20 and 200 microg/g BW. suppressed antibody-forming cell (AFC) numbers, superoxide production, and host resistance against bacteria. In contrast, exposure to the low affinity aryl hydrocarbon receptor (AhR) agonist, benzo[e]pyrene (BeP), neither induced CYP1A expression nor altered immune function. Given the lack of immunosuppressive effects produced by BeP, and the fact that exposure to the AhR antagonist (and CYP1A inhibitor) alpha-naphthoflavone (ANF) ameliorated the suppressive effects of BaP upon AFC numbers, the AhR pathway (including CYP1A-mediated production of reactive BaP metabolites) appears important in mediating BaP-induced immunotoxicity in fish, as in mammals. In the past, the medaka has proven a successful model for assessing carcinogenic agents. These studies have demonstrated its utility for also determining the immunosuppressive effects of an important aquatic contaminant.

  1. Toxic effects of male Perna viridis gonad exposed to BaP, DDT and their mixture: A metabolomic and proteomic study of the underlying mechanism.

    Science.gov (United States)

    Song, Qinqin; Zheng, Pengfei; Qiu, Liguo; Jiang, Xiu; Zhao, Hongwei; Zhou, Hailong; Han, Qian; Diao, Xiaoping

    2016-01-01

    Benzo(a)pyrene and dichlorodiphenyltrichloroethane are typical persistent organic pollutants, and also the widespread environmental estrogens with known toxicity towards green mussels Perna viridis. In this study, the toxicological effects of BaP and DDT and their mixture were assessed in green mussel gonads using proteomic and metabolomic approaches. Metabolomics by NMR spectroscopy revealed that BaP did not show obvious metabolite changes in the gonad of male green mussel. DDT mainly caused some disturbance of osmotic regulation and energy metabolism by changing BCAAs, alanine, threonine, arginine, etc., unknown metabolite (3.53 ppm), glycine, homarine and ATP at different levels. However, the mixture of BaP and DDT mainly caused some disturbance in osmotic regulation and energy metabolism by differentially altering branched chain amino acids, glutamate, alanine, arginine, unknown metabolite (3.53 ppm), glycine, 4-aminobutyrate, dimethylglycine, homarine and ATP. The results suggest that DDT alone may cause most of metabolites changes in the mixture exposed male mussel gonad, and the results also show that the male P. viridis gonad was more sensitive to DDT than BaP exposures. Proteomic study showed that BaP, DDT and their mixture may have different modes of action. Proteomic responses revealed that BaP induced signal transduction, oxidative stress, spermatogenesis, etc. in the male green mussel gonad; whereas DDT exposure altered proteins that were associated with signal transduction, oxidative stress, cytoskeleton and cell structure, cellular organization, energy metabolism, etc. However, the mixture of BaP and DDT affected proteins related to cytoskeleton and cell structure, oxidative stress, cellular organization, etc. This research demonstrated that metabolomic and proteomic approaches could better elucidate the underlying mechanism of environmental pollutants gonad toxicity.

  2. PCB126 enhanced the genotoxicity of BaP in HepG2 cells by modulating metabolic enzyme and DNA repair activities.

    Science.gov (United States)

    Wei, Wei; Zhang, Chi; Liu, Ai-Lin; Xie, Shao-Hua; Chen, Xue-Min; Lu, Wen-Qing

    2009-09-10

    Both of benzo(a)pyrene (BaP) and 3,3',4,4',5-pentachlorobiphenyl (PCB126) are ubiquitous and persistent environmental pollutants. These two chemicals coexist in various environmental media and human samples and thus may have combined effects on human health. However, the toxic effects and related mechanism of co-exposure to BaP and PCB126 remain unknown. In a series of experiments using the HepG2 cells exposed to BaP (50microM) or/and PCB126 (0.01, 0.1, 1 and 10nM), we measured the rate of micronucleus (MN) formation, CYP1A1 activity and expression of nucleotide excision repair (NER) proteins (XPA and XPC). We found that the exposure to BaP or PCB126 alone could effectively increase the CYP1A1 activity and the XPA expression. BaP alone had a profound enhancement of MN formation. Compared with BaP alone, co-exposure to both BaP and PCB126 significantly enhanced the CYP1A1 activity and the formation of MN but reduced the expression of both XPA and XPC. The synergistic effect of PCB126 on BaP-induced MN formation was inhibited by alpha-naphthoflavone (ANF), an inhibitor of CYP1A1. Our findings suggest that PCB126 may enhance BaP-induced DNA damage and genotoxicity by increasing cytochrome P450 1A activity and decreasing the NER capacity.

  3. Source apportionment of elevated BaP concentrations in PM10 aerosols in an alpine valley in Austria

    Science.gov (United States)

    Bauer, Heidi; Puxbaum, Hans; Jankowski, Nicole; Sampaio Cordeiro Wagner, Lylian

    2010-05-01

    INTRODUCTION: In a village situated at 1215 m a.s.l. in a natural preserve in an Austrian alpine valley elevated BaP concentrations have been measured in the last years. A highly frequented highway leading from Italy to Germany passes near the village. Monthly means of particulate BaP concentrations show a clear seasonal trend with values below 1 ng/m³ during the warmer months and with concentrations up to 9 ng/m³ in the cold season. Annual averages in the years 2000 - 2005 ranged between 1.4 and 2.8 ng/m³ - much higher than the EU target value of 1 ng/m³. We used a macrotracer model developed at the Vienna University of Technology to determine the contributions of the sources for BaP emissions, which were mainly space heating with wood and traffic from the highway. EXPERIMENTAL: The macrotracer concept is a nine component model to derive source contribution and explains 80-100% of PM10 aerosols in Austria. The amount of traffic exhaust is derived by using EC as tracer, whereas EC produced by wood burning is subtracted, the amount of wood smoke is derived by the anhydro-sugar levoglucosan and the ratio between the anhydro-sugars levoglucosan and mannosan. For the source apportionment of BaP the applied factors reflect on the one hand the composition of the automotive fleet in Austria and on the other hand the composition of the fire wood in the region. Filter samples collected with a high volume sampler in winter were analyzed for PM10 aerosol mass, total, organic, elemental and carbonate carbon, HULIS, anhydro-sugars, polyols and ions (major ions and organic acids) and PAHs. In the same way emission samples taken at a motor test stand and at a test stand for wood combustion were analyzed (Schmidl et al. 2008). The saccharides were determined using high pH anion exchange and pulsed amperometry (HPAE-PAD). Details of the analytical method are given in Iinuma et al., 2009. Elemental and organic carbon were determined with a thermal-optical instrument (Sunset lab

  4. Exome sequencing identifies frequent inactivating mutations in BAP1, ARID1A and PBRM1 in intrahepatic cholangiocarcinomas

    Science.gov (United States)

    Selaru, Florin M; Streppel, Mirte M; Lucas, Donald J; Niknafs, Noushin; Guthrie, Violeta Beleva; Maitra, Anirban; Argani, Pedram; Offerhaus, G Johan A; Roa, Juan Carlos; Roberts, Lewis R; Gores, Gregory J; Popescu, Irinel; Alexandrescu, Sorin T; Dima, Simona; Fassan, Matteo; Simbolo, Michele; Mafficini, Andrea; Capelli, Paola; Lawlor, Rita T; Ruzzenente, Andrea; Guglielmi, Alfredo; Tortora, Giampaolo; de Braud, Filippo; Scarpa, Aldo; Jarnagin, William; Klimstra, David; Karchin, Rachel; Velculescu, Victor E; Hruban, Ralph H; Vogelstein, Bert; Kinzler, Kenneth W; Papadopoulos, Nickolas; Wood, Laura D

    2014-01-01

    Through exomic sequencing of 32 intrahepatic cholangiocarcinomas, we discovered frequent inactivating mutations in multiple chromatin-remodeling genes (including BAP1, ARID1A and PBRM1), and mutation in one of these genes occurred in almost half of the carcinomas sequenced. We also identified frequent mutations at previously reported hotspots in the IDH1 and IDH2 genes encoding metabolic enzymes in intrahepatic cholangiocarcinomas. In contrast, TP53 was the most frequently altered gene in a series of nine gallbladder carcinomas. These discoveries highlight the key role of dysregulated chromatin remodeling in intrahepatic cholangiocarcinomas. PMID:24185509

  5. Modulation of adult rat benzo(a)pyrene (BaP) metabolism and DNA adduct formation by neonatal diethylstilbestrol (DES) exposure.

    Science.gov (United States)

    Ramesh, Aramandla; Inyang, Frank; Knuckles, Maurice E

    2004-12-01

    This study seeks to elucidate the role of diethylstilbestrol (DES), a synthetic estrogen on benzo(a)pyrene (BaP) metabolism in the male rat reproductive tissues. Offspring of timed-pregnant Sprague-Dawley rats were neonatally treated on days 2, 4, and 6 post-partum with 1.45 micromol/kg of DES. Ten weeks after birth, the adult rats were challenged with radiolabeled benzo(a)pyrene (3H BaP) (10 micromol/kg) and the rats were sacrificed 2 h after BaP exposure. Prostrate, testis, lung, liver, urine and feces samples were collected and extracted using a mixture of H2O, MeOH and CHCl3. The extracts were analyzed by reverse phase HPLC. The concentrations of BaP organic metabolites in DES rats were lower compared to controls (vehicle-treated rats). On the other hand, concentrations of aqueous metabolites were significantly increased in DES treated animals. The toxication to detoxication ratios were significantly decreased in DES rats compared to controls. This trend is also reflected in the decreased concentrations of BaP-DNA adducts in DES rats. Collectively these results suggest that DES is capable of modulating the metabolic pathway of BaP towards detoxification thereby preventing the manifestation of toxicity.

  6. P-glycoprotein and CYP1A protein expression patterns in Nile tilapia (Oreochromis niloticus) tissues after waterborne exposure to benzo(a)pyrene (BaP).

    Science.gov (United States)

    Costa, Joana; Reis-Henriques, Maria Armanda; Wilson, Jonathan M; Ferreira, Marta

    2013-09-01

    The protein levels and tissue distribution patterns of P-glycoprotein (Pgp) and cytochrome P450 (CYP1A) were investigated in Nile tilapia (Oreochromis niloticus) after waterborne exposure to different benzo(a)pyrene (BaP) concentrations, using immunochemical approaches. The Pgp mammalian monoclonal antibody (mAb) C219 cross reacted with a ∼170kDa protein, almost exclusively localized to the bile canaliculi, while probing with the Pgp mammalian mAb C494, resulted in a positive reaction in liver, gills and intestine of Nile tilapia and covered a wider set of cell types. Levels of Pgp expression were not altered after in vivo exposure to BaP. CYP1A, detected with the mAb C10-7, reacted positively in liver, gills and intestine and followed a BaP dose-dependent fold induction. Taken together, these results indicate that CYP1A is involved in BaP metabolism in liver, gills and intestine, however, further studies are needed to elucidate the possible interaction of the efflux protein Pgp with BaP and/or its metabolites.

  7. A fluorescence enhancement-based label-free homogeneous immunoassay of benzo[a]pyrene (BaP) in aqueous solutions.

    Science.gov (United States)

    Li, Taihua; Choi, Yo Han; Shin, Yong-Beom; Kim, Hwa-Jung; Kim, Min-Gon

    2016-05-01

    A fluorescence enhancement-based immunoassay has been developed for the detection of the polycyclic aromatic hydrocarbons (PAH), benzo[a]pyrene (BaP), in aqueous solutions. The results of this study show that BaP, which inefficiently fluoresces in aqueous solution, displays enhanced fluorescence when bound to the anti-BaP antibody (anti-BaP), as part of a label-free immunoassay system. Binding to anti-BaP results in a 3.12-fold increase in the fluorescence intensity of BaP, which emits at 435 nm when excited at 280 nm, due to the hydrophobic interaction and fluorescence resonance energy transfer (FRET) between antibody and antigen. As result of this phenomenon, the antibody-based fluorescence immunoassay system can be used to detect BaP specifically with a limit of detection (LOD) of 0.06 ng mL(-1). Finally, extraction recoveries of BaP from spiked wheat and barley samples were found to be in the range of 80.5-87.0% and 92.9-92.1%, respectively.

  8. Multiplicação in vitro de Eucalyptus grandis sob estímulo com benzilaminopurina In vitro multiplication of Eucalyptus grandis under BAP pulse

    Directory of Open Access Journals (Sweden)

    Wirifran Fernandes de Andrade

    2006-12-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos do estímulo com benzilaminopurina (BAP na multiplicação in vitro de Eucalyptus grandis. Foram avaliadas as interações entre concentrações de BAP (0, 200, 400 e 600 mg L-1, tempo de exposição (1, 2 e 3 horas, pH (3 e 5,8 da solução e alterações morfológicas dos explantes. Semanalmente, foi determinada a massa da matéria fresca dos explantes. Aos 21 dias de cultura, foram avaliados: o número de brotações por tratamento, o número de brotações obtidas por explante (taxa de multiplicação, e foi realizado o resgate das plântulas para avaliação histológica. O pH não apresentou interação com os demais fatores estudados. Os tratamentos com BAP a 200 mg L-1, durante 1 e 2 horas, apresentaram-se como os mais indicados na multiplicação do E. grandis. Houve intensificação da divisão celular, no parênquima cortical e no procâmbio, representada pelo surgimento de meristemóides, em resposta aos tratamentos com 200 mg L-1 de BAP, durante 1 e 2 horas. O estímulo com BAP na multiplicação in vitro de E. grandis é viável.The objective of this work was to evaluate the effect of pulse on in vitro multiplication of Eucalyptus grandis. Interactions were evaluated among BAP concentrations (0, 200, 400 and 600 mg L-1, exposure time (1, 2 and 3 hours, pH values (3 and 5.8, and explant morphological changes. Fresh weight of the explants was determined weekly. At 21 days of culture, valuations were made of the number of shootings per treatment, number of shootings obtained per explant (multiplication rate, and seedlings rescue was accomplished for histological analysis. The pH values did not present any interaction with the other factors. The most significant treatments on E. grandis shoot multiplications were 200 mg L-1 of 6-BAP during 1 and 2 hours. There was an intensification of cell division in the cortical parenchyma and procambium, represented by the arising of meristems in

  9. Concentração de bap e a eficiência de micropropagação de bananeira tetraplóide (grupo AAAB Bap concentration and tetraploid banana micropropagation efficiency (AAAB group

    Directory of Open Access Journals (Sweden)

    Roberto Pedroso de Oliveira

    2001-03-01

    Full Text Available Com o objetivo de desenvolver um procedimento eficiente para a produção de mudas de bananeiras tetraplóides (Musa sp. cv. FHIA-01, grupo AAAB estudou-se o efeito do clone no desenvolvimento in vitro dos explantes e as taxas de contaminação, multiplicação e nível de oxidação em meios de cultura contendo cinco concentrações de BAP. Na fase de introdução in vitro dos explantes, foi observada elevada taxa de contaminação (22,32%, causada, principalmente, por bactérias. No entanto, os níveis de contaminação decresceram na seqüência dos subcultivos, tendo sido de 1,07% no oitavo subcultivo. As maiores taxas de multiplicação foram obtidas no meio de cultura MS com 4,0 mg L-1 de BAP, em média de 2,65 plântulas por subcultivo, possibilitando a produção estimada de 584 plântulas de FHIA-01 por explante inicial no sexto e 3451 no oitavo subcultivo. Em função do clone, houve a produção estimada de 305 a 4497 plântulas por explante inicial, após oito subculturas. A eficiência de aclimatização das plântulas foi de 94%, não sendo encontrados variantes somaclonais em condições de casa-de-vegetação.The objective of the present work was to establish an efficient procedure for the in vitro production of tetraploid banana plantlets (Musa sp. cv. FHIA-01, AAAB group. The cloning effect on the in vitro development of the explants, the rates of contamination and multiplication, and the level of oxidation in culture media with five benzylaminopurine (BAP concentrations were studied. In the phase of in vitro introduction of explants, a high level of contamination (22.32%, caused mainly by bacteria, was observed. However, the degree of contamination decreased along the subcultures, reaching 1.07% in the eighth subculture. Higher multiplication rates were obtained, averaging 2.65 per subculture, on the MS media supplemented with 4.0 mg L-1 BAP, leading to an estimated production of 584 and 3451 plantlets/initial explant after

  10. Titanium dioxide nanoparticles provide protection against polycyclic aromatic hydrocarbon BaP and chrysene-induced perturbation of DNA repair machinery: A computational biology approach.

    Science.gov (United States)

    Dhasmana, Anupam; Jamal, Qazi Mohd Sajid; Gupta, Richa; Siddiqui, Mohd Haris; Kesari, Kavindra Kumar; Wadhwa, Gulshan; Khan, Saif; Haque, Shafiul; Lohani, Mohtashim

    2016-07-01

    We examined the interaction of polycyclic hydrocarbons (PAHs) like benzo-α-pyrene (BaP), chrysene, and their metabolites 7,8-dihydro-7,8-dihydroxybenzo(a)pyrene,9,10-oxide (BPDE) and chrysene 1,2-diol-3,4-epoxide-2 (CDE), with the enzymes involved in DNA repair. We investigated interaction of 120 enzymes with PAHs and screened out 40 probable targets among DNA repair enzymes, on the basis of higher binding energy than positive control. Out of which, 20 enzymes lose their function in the presence of BaP, chrysene, and their metabolites, which may fetter DNA repair pathways resulting in damage accumulation and finally leading to cancer formation. We propose the use of nanoparticles as a guardian against the PAH's induced toxicity. PAHs enter the cell via aryl hydrocarbon receptor (AHR). TiO2 NP showed a much higher docking score with AHR (12,074) as compared with BaP and chrysene with AHR (4,600 and 4,186, respectively), indicating a preferential binding of TiO2 NP with the AHR. Further, docking of BaP and chrysene with the TiO2 NP bound AHR complex revealed their strong adsorption on TiO2 NP itself, and not on their original binding site (at AHR). TiO2 NPs thereby prevent the entry of PAHs into the cell via AHR and hence protect cells against the deleterious effects induced by PAHs.

  11. Significance of measuring oxidative stress in lifestyle-related diseases from the viewpoint of correlation between d-ROMs and BAP in Japanese subjects.

    Science.gov (United States)

    Fukui, Toshiki; Yamauchi, Kazuhiro; Maruyama, Mie; Yasuda, Tadashi; Kohno, Masakazu; Abe, Youichi

    2011-09-01

    In recent years, oxidative stress has been postulated to be an important factor in the pathogenesis and development of lifestyle-related diseases. In this study, we investigated the association between the derivatives of reactive oxygen metabolites (d-ROMs), as an index of products of reactive oxygen species (ROS), and biological antioxidant potential (BAP), as an index of antioxidant potential. We also investigated the associations between d-ROMs or BAP and the risk factors for lifestyle-related diseases or metabolic syndrome-associated factors to evaluate their usefulness in preventive medicine. There were 442 subjects who underwent health checkup examination in our facilities. In addition to standard medical checkup items, we analyzed d-ROMs, BAP, brachial-ankle pulse wave velocity, high-sensitivity C-reactive protein level and visceral fat area (VFA) visualized on a computed tomography scan. The mean d-ROM value in females was significantly higher than that in males. There was a positive correlation between the d-ROM and VFA levels. On correlation analysis, there was a negative correlation between the d-ROM and creatinine levels. As factors that influence d-ROMs, the level of VFA was selected, suggesting the significance of oxidative stress measurement with d-ROMs. In addition, there was a positive correlation between d-ROMs and BAP values. Further research is required to resolve whether increased production of ROS or the antioxidant potential that can compensate for such an increase of ROS is more important in vivo.

  12. Burial effects of organic coatings on the heterogeneous reactivity of particle-borne benzo[a]pyrene (BaP) toward ozone.

    Science.gov (United States)

    Zhou, S; Lee, A K Y; McWhinney, R D; Abbatt, J P D

    2012-07-01

    With an aerosol flow tube coupled to an Aerodyne aerosol mass spectrometer (AMS), room temperature (296 ± 3 K) kinetics studies have been performed on the reaction of gas-phase ozone with benzo[a]pyrene (BaP) adsorbed in submonolayer amounts to dry ammonium sulfate (AS) particles. Three organic substances, i.e., bis(2-ethylhexyl)sebacate (BES, liquid), phenylsiloxane oil (PSO, liquid), and eicosane (EC, solid), were used to coat BaP-AS particles to investigate the effects of such organic coatings on the heterogeneous reactivity of PAHs toward ozone. All the reactions of particle-borne BaP with excess ozone exhibit pseudo-first-order kinetics in terms of BaP loss, and reactions with a liquid organic coating proceed by the Langmuir-Hinshelwood (L-H) mechanism. Liquid organic coatings did not significantly affect the kinetics, consistent with the ability of reactants to rapidly diffuse through the organic coating. In contrast, the heterogeneous reactivity of BaP was reduced substantially by a thin (4-8 nm), solid EC coating and entirely suppressed by thick (10-80 nm) coatings, presumably because of slow diffusion through the organic layer. Although the heterogeneous reactivity of surface-bound PAHs is extremely rapid in the atmosphere, this work is the first to experimentally demonstrate a mechanism by which the lifetime of PAHs may be significantly prolonged, permitting them to undergo long-range transport to remote locations.

  13. Influence of dietary fat type on benzo(a)pyrene [B(a)P] biotransformation in a B(a)P-induced mouse model of colon cancer.

    Science.gov (United States)

    Diggs, Deacqunita L; Myers, Jeremy N; Banks, Leah D; Niaz, Mohammad S; Hood, Darryl B; Roberts, L Jackson; Ramesh, Aramandla

    2013-12-01

    In the US alone, around 60,000 lives/year are lost due to colon cancer. Diet and environment have been implicated in the development of sporadic colon tumors. The objective of this study was to determine how dietary fat potentiates the development of colon tumors through altered B(a)P biotransformation, using the Adenomatous polyposis coli with Multiple intestinal neoplasia mouse model. Benzo(a)pyrene was administered to mice through tricaprylin, and unsaturated (USF; peanut oil) and saturated (SF; coconut oil) fats at doses of 50 and 100 μg/kg via oral gavage over a 60-day period. Blood, colon, and liver were collected at the end of exposure period. The expression of B(a)P biotransformation enzymes [cytochrome P450 (CYP)1A1, CYP1B1 and glutathione-S-transferase] in liver and colon were assayed at the level of protein, mRNA and activities. Plasma and tissue samples were analyzed by reverse phase high-performance liquid chromatography for B(a)P metabolites. Additionally, DNA isolated from colon and liver tissues was analyzed for B(a)P-induced DNA adducts by the (32)P-postlabeling method using a thin-layer chromatography system. Benzo(a)pyrene exposure through dietary fat altered its metabolic fate in a dose-dependent manner, with 100 μg/kg dose group registering an elevated expression of B(a)P biotransformation enzymes, and greater concentration of B(a)P metabolites, compared to the 50 μg/kg dose group (Pcolon and liver, whose concentrations also registered a dose-dependent increase. These metabolites were found to bind with DNA and form B(a)P-DNA adducts, which may have contributed to colon tumors in a subchronic exposure regimen.

  14. Noncovalent interactions of a benzo[a]pyrene diol epoxide with DNA base pairs: insight into the formation of adducts of (+)-BaP DE-2 with DNA.

    Science.gov (United States)

    Hargis, Jacqueline C; Schaefer, Henry F; Houk, K N; Wheeler, Steven E

    2010-02-01

    Noncovalent complexes of a tumorigenic benzo[a]pyrene diol epoxide with the guanine-cytosine (GC) and adenine-thymine (AT) base pairs have been examined computationally. (+)-BaP DE-2 forms covalent adducts with DNA via nucleophilic attack on the (+)-BaP DE-2 epoxide. Computational results predict five thermodynamically accessible complexes of AT with (+)-BaP DE-2 that are compatible with intact DNA. Among these, two are expected to lead to adenine adducts. In the lowest energy AT...(+)-BaP DE-2 complex, which has a gas-phase interaction energy of -20.9 kcal mol(-1), the exocyclic NH(2) of adenine is positioned for backside epoxide attack and formation of a trans adduct. The most energetically favorable complex leading to formation of a cis ring-opened adduct lies only 0.6 kcal mol(-1) higher in energy. For GC...(+)-BaP DE-2, there are only two thermodynamically accessible complexes. The higher-lying complex, bound in the gas phase by 24.4 kcal mol(-1) relative to separated GC and (+)-BaP DE-2, would lead to a trans ring-opened N(2)-guanine adduct. In the global minimum energy GC...(+)-BaP DE-2 complex, bound by 27.3 kcal mol(-1), the exocyclic NH(2) group of cytosine is positioned for cis epoxide addition. However, adducts of (+)-BaP DE-2 with cytosine are rarely observed experimentally. The paucity of cytosine adducts, despite the predicted thermodynamic stability of this GC...(+)-BaP DE-2 complex, is attributed to the electrostatic destabilization of the benzylic cation intermediate thought to precede cis addition.

  15. Changes in the Metabolome of Picea balfouriana Embryogenic Tissues That Were Linked to Different Levels of 6-BAP by Gas Chromatography-Mass Spectrometry Approach.

    Science.gov (United States)

    Li, Q F; Wang, J H; Pulkkinen, P; Kong, L S

    2015-01-01

    Embryogenic cultures of Picea balfouriana, which is an important commercial species for reforestation in Southern China, easily lose their embryogenic ability during long-term culture. Embryogenic tissue that proliferated at lower concentrations (3.6 μM and 2.5 μM) of 6-benzylaminopurine (6-BAP) were more productive, and generated 113 ± 6 and 89 ± 3 mature embryos per 100 mg embryogenic tissue, respectively. A metabolomic approach was used to study the changes in metabolites linked to embryogenic competence related to three different 6-BAP concentrations (2.5 μM, 3.6 μM, and 5 μM). A total of 309 compounds were obtained, among which 123 metabolites mapped to Kyoto Encyclopedia of Genes and genomes (KEGG) pathways. The levels of 35 metabolites were significantly differentially regulated among the three 6-BAP treatments, and 32 metabolites differed between the 2.5 μM and 5 μM treatments. A total of 17 metabolites appeared only once among the three comparisons. The combination of a score plot and a loading plot showed that in the samples with higher embryogenic ability (3.6 μM and 2.5 μM), up-regulated metabolites were mostly amino acids and down-regulated metabolites were mostly primary carbohydrates (especially sugars). These results suggested that 6-BAP may influence embryogenic competence by nitrogen metabolism, which could cause an increase in amino acid levels and higher amounts of aspartate, isoleucine, and leucine in tissues with higher embryogenic ability. Furthermore, we speculated that 6-BAP may affect the amount of tryptophan in tissues, which would change the indole-3-acetic acid levels and influence the embryogenic ability.

  16. Seasonal variation of benzo(a)pyrene in the Spanish airborne PM10. Multivariate linear regression model applied to estimate BaP concentrations.

    Science.gov (United States)

    Callén, M S; López, J M; Mastral, A M

    2010-08-15

    The estimation of benzo(a)pyrene (BaP) concentrations in ambient air is very important from an environmental point of view especially with the introduction of the Directive 2004/107/EC and due to the carcinogenic character of this pollutant. A sampling campaign of particulate matter less or equal than 10 microns (PM10) carried out during 2008-2009 in four locations of Spain was collected to determine experimentally BaP concentrations by gas chromatography mass-spectrometry mass-spectrometry (GC-MS-MS). Multivariate linear regression models (MLRM) were used to predict BaP air concentrations in two sampling places, taking PM10 and meteorological variables as possible predictors. The model obtained with data from two sampling sites (all sites model) (R(2)=0.817, PRESS/SSY=0.183) included the significant variables like PM10, temperature, solar radiation and wind speed and was internally and externally validated. The first validation was performed by cross validation and the last one by BaP concentrations from previous campaigns carried out in Zaragoza from 2001-2004. The proposed model constitutes a first approximation to estimate BaP concentrations in urban atmospheres with very good internal prediction (Q(CV)(2)=0.813, PRESS/SSY=0.187) and with the maximal external prediction for the 2001-2002 campaign (Q(ext)(2)=0.679 and PRESS/SSY=0.321) versus the 2001-2004 campaign (Q(ext)(2)=0.551, PRESS/SSY=0.449).

  17. Changes in the Metabolome of Picea balfouriana Embryogenic Tissues That Were Linked to Different Levels of 6-BAP by Gas Chromatography-Mass Spectrometry Approach.

    Directory of Open Access Journals (Sweden)

    Q F Li

    Full Text Available Embryogenic cultures of Picea balfouriana, which is an important commercial species for reforestation in Southern China, easily lose their embryogenic ability during long-term culture. Embryogenic tissue that proliferated at lower concentrations (3.6 μM and 2.5 μM of 6-benzylaminopurine (6-BAP were more productive, and generated 113 ± 6 and 89 ± 3 mature embryos per 100 mg embryogenic tissue, respectively. A metabolomic approach was used to study the changes in metabolites linked to embryogenic competence related to three different 6-BAP concentrations (2.5 μM, 3.6 μM, and 5 μM. A total of 309 compounds were obtained, among which 123 metabolites mapped to Kyoto Encyclopedia of Genes and genomes (KEGG pathways. The levels of 35 metabolites were significantly differentially regulated among the three 6-BAP treatments, and 32 metabolites differed between the 2.5 μM and 5 μM treatments. A total of 17 metabolites appeared only once among the three comparisons. The combination of a score plot and a loading plot showed that in the samples with higher embryogenic ability (3.6 μM and 2.5 μM, up-regulated metabolites were mostly amino acids and down-regulated metabolites were mostly primary carbohydrates (especially sugars. These results suggested that 6-BAP may influence embryogenic competence by nitrogen metabolism, which could cause an increase in amino acid levels and higher amounts of aspartate, isoleucine, and leucine in tissues with higher embryogenic ability. Furthermore, we speculated that 6-BAP may affect the amount of tryptophan in tissues, which would change the indole-3-acetic acid levels and influence the embryogenic ability.

  18. Seasonal variation of benzo(a)pyrene in the Spanish airborne PM10. Multivariate linear regression model applied to estimate BaP concentrations

    International Nuclear Information System (INIS)

    The estimation of benzo(a)pyrene (BaP) concentrations in ambient air is very important from an environmental point of view especially with the introduction of the Directive 2004/107/EC and due to the carcinogenic character of this pollutant. A sampling campaign of particulate matter less or equal than 10 microns (PM10) carried out during 2008-2009 in four locations of Spain was collected to determine experimentally BaP concentrations by gas chromatography mass-spectrometry mass-spectrometry (GC-MS-MS). Multivariate linear regression models (MLRM) were used to predict BaP air concentrations in two sampling places, taking PM10 and meteorological variables as possible predictors. The model obtained with data from two sampling sites (all sites model) (R2 = 0.817, PRESS/SSY = 0.183) included the significant variables like PM10, temperature, solar radiation and wind speed and was internally and externally validated. The first validation was performed by cross validation and the last one by BaP concentrations from previous campaigns carried out in Zaragoza from 2001-2004. The proposed model constitutes a first approximation to estimate BaP concentrations in urban atmospheres with very good internal prediction (QCV2=0.813, PRESS/SSY = 0.187) and with the maximal external prediction for the 2001-2002 campaign (Qext2=0.679 and PRESS/SSY = 0.321) versus the 2001-2004 campaign (Qext2=0.551, PRESS/SSY = 0.449).

  19. Ação do BAP na regeneração in vitro de Blc Owen Holmes Ponkan x Brassavola digbiana nº 2 BAP (6-benzylaminopurine in the regeneration in vitro of Blc Owen Holmes Ponkan x Brassavola digbiana nº 2

    Directory of Open Access Journals (Sweden)

    Luciana Giatti

    2007-10-01

    Full Text Available A Cattleya é considerada a mais bela e procurada de todas as orquídeas. Esta demanda cria a necessidade do desenvolvimento de técnicas mais eficazes de propagação que ofereçam maior número de indivíduos em período de tempo e espaço físico reduzido, além de manterem a uniformidade e a identidade genotípica para atender às exigências do mercado. Objetivou-se neste trabalho avaliar a regeneração de meristemas provenientes de gemas cultivadas in vitro do híbrido Blc Owen Holmes Ponkan x Brassavola digbiana nº 2, em diferentes concentrações de BAP (6-benzilaminopurina. Gemas com 37 dias de cultivo foram inoculadas em meio de cultura líquido com reguladores vegetais, nos respectivos tratamentos: T1 - MS líquido + água de coco; T2 - MS + água de coco + 0,1 mg L-1 ANA (ácido naftaleno acético + 0,2 mg L-1 BAP; T3 - MS + água de coco + 0,1 mg L-1 ANA + 0,5 mg L-1 BAP; T4 - MS + água de coco + 0,1 mg L-1 ANA + 1,0 mg L-1 BAP. Os subcultivos se realizaram aos 18, 32, 62, 93, 123, 153 e 184 dias e as avaliações foram feitas observando-se a porcentagem de sobrevivência, contaminação, oxidação e regeneração dos explantes. Observou-se que 86,7% dos explantes sobreviveram e aos 93 dias ocorreu o primeiro sinal de regeneração. BAP (1,0 mg L-1 promoveu resultados satisfatórios com a formação de 132 perfilhos aos 184 dias de cultivo, potencializando a técnica para produção em escala comercial dessa espécie.Cattleya is considered the most beautiful and searched among all orchids. This fact creates the need of developing more effective propagation techniques to become available a greater number of individuals in reduced periods of time and physical space, besides maintenance of uniformity and genotipic identity to meet the needs of the market. The objective of this work was to evaluate the regeneration of the cultivated shoot meristematic in vitro of the hybrid Blc Owen Holmes Ponkan x Brassavola digbiana nº 2 in the

  20. A Tumor-Specific Neo-Antigen Caused by a Frameshift Mutation in BAP1 Is a Potential Personalized Biomarker in Malignant Peritoneal Mesothelioma

    Science.gov (United States)

    Lai, Jun; Zhou, Zhan; Tang, Xiao-Jing; Gao, Zhi-Bin; Zhou, Jie; Chen, Shu-Qing

    2016-01-01

    Malignant peritoneal mesothelioma (MPM) is an aggressive rare malignancy associated with asbestos exposure. A better understanding of the molecular pathogenesis of MPM will help develop a targeted therapy strategy. Oncogene targeted depth sequencing was performed on a tumor sample and paired peripheral blood DNA from a patient with malignant mesothelioma of the peritoneum. Four somatic base-substitutions in NOTCH2, NSD1, PDE4DIP, and ATP10B and 1 insert frameshift mutation in BAP1 were validated by the Sanger method at the transcriptional level. A 13-amino acids neo-peptide of the truncated Bap1 protein, which was produced as a result of this novel frameshift mutation, was predicted to be presented by this patient’s HLA-B protein. The polyclonal antibody of the synthesized 13-mer neo-peptide was produced in rabbits. Western blotting results showed a good antibody-neoantigen specificity, and Immunohistochemistry (IHC) staining with the antibody of the neo-peptide clearly differentiated neoplastic cells from normal cells. A search of the Catalogue of Somatic Mutations in Cancer (COSMIC) database also revealed that 53.2% of mutations in BAP1 were frameshift indels with neo-peptide formation. An identified tumor-specific neo-antigen could be the potential molecular biomarker for personalized diagnosis to precisely subtype rare malignancies such as MPM. PMID:27187383

  1. Systemic control of cell division and endoreduplication by NAA and BAP by modulating CDKs in root tip cells of Allium cepa.

    Science.gov (United States)

    Tank, Jigna G; Thaker, Vrinda S

    2014-01-01

    Molecular mechanism regulated by auxin and cytokinin during endoreduplication, cell division, and elongation process is studied by using Allium cepa roots as a model system. The activity of CDK genes modulated by auxin and cytokinin during cell division, elongation, and endoreduplication process is explained in this research work. To study the significance of auxin and cytokinin in the management of cell division and endoreduplication process in plant meristematic cells at molecular level endoreduplication was developed in root tips of Allium cepa by giving colchicine treatment. There were inhibition of vegetative growth, formation of c-tumor at root tip, and development of endoreduplicated cells after colchicine treatment. This c-tumor was further treated with NAA and BAP to reinitiate vegetative growth in roots. BAP gave positive response in reinitiation of vegetative growth of roots from center of c-tumor. However, NAA gave negative response in reinitiation of vegetative growth of roots from c-tumor. Further, CDKs gene expression analysis from normal, endoreduplicated, and phytohormone (NAA or BAP) treated root tip was done and remarkable changes in transcription level of CDK genes in normal, endoreduplicated, and phytohormones treated cells were observed.

  2. A Tumor-Specific Neo-Antigen Caused by a Frameshift Mutation in BAP1 Is a Potential Personalized Biomarker in Malignant Peritoneal Mesothelioma.

    Science.gov (United States)

    Lai, Jun; Zhou, Zhan; Tang, Xiao-Jing; Gao, Zhi-Bin; Zhou, Jie; Chen, Shu-Qing

    2016-05-14

    Malignant peritoneal mesothelioma (MPM) is an aggressive rare malignancy associated with asbestos exposure. A better understanding of the molecular pathogenesis of MPM will help develop a targeted therapy strategy. Oncogene targeted depth sequencing was performed on a tumor sample and paired peripheral blood DNA from a patient with malignant mesothelioma of the peritoneum. Four somatic base-substitutions in NOTCH2, NSD1, PDE4DIP, and ATP10B and 1 insert frameshift mutation in BAP1 were validated by the Sanger method at the transcriptional level. A 13-amino acids neo-peptide of the truncated Bap1 protein, which was produced as a result of this novel frameshift mutation, was predicted to be presented by this patient's HLA-B protein. The polyclonal antibody of the synthesized 13-mer neo-peptide was produced in rabbits. Western blotting results showed a good antibody-neoantigen specificity, and Immunohistochemistry (IHC) staining with the antibody of the neo-peptide clearly differentiated neoplastic cells from normal cells. A search of the Catalogue of Somatic Mutations in Cancer (COSMIC) database also revealed that 53.2% of mutations in BAP1 were frameshift indels with neo-peptide formation. An identified tumor-specific neo-antigen could be the potential molecular biomarker for personalized diagnosis to precisely subtype rare malignancies such as MPM.

  3. A Tumor-Specific Neo-Antigen Caused by a Frameshift Mutation in BAP1 Is a Potential Personalized Biomarker in Malignant Peritoneal Mesothelioma.

    Science.gov (United States)

    Lai, Jun; Zhou, Zhan; Tang, Xiao-Jing; Gao, Zhi-Bin; Zhou, Jie; Chen, Shu-Qing

    2016-01-01

    Malignant peritoneal mesothelioma (MPM) is an aggressive rare malignancy associated with asbestos exposure. A better understanding of the molecular pathogenesis of MPM will help develop a targeted therapy strategy. Oncogene targeted depth sequencing was performed on a tumor sample and paired peripheral blood DNA from a patient with malignant mesothelioma of the peritoneum. Four somatic base-substitutions in NOTCH2, NSD1, PDE4DIP, and ATP10B and 1 insert frameshift mutation in BAP1 were validated by the Sanger method at the transcriptional level. A 13-amino acids neo-peptide of the truncated Bap1 protein, which was produced as a result of this novel frameshift mutation, was predicted to be presented by this patient's HLA-B protein. The polyclonal antibody of the synthesized 13-mer neo-peptide was produced in rabbits. Western blotting results showed a good antibody-neoantigen specificity, and Immunohistochemistry (IHC) staining with the antibody of the neo-peptide clearly differentiated neoplastic cells from normal cells. A search of the Catalogue of Somatic Mutations in Cancer (COSMIC) database also revealed that 53.2% of mutations in BAP1 were frameshift indels with neo-peptide formation. An identified tumor-specific neo-antigen could be the potential molecular biomarker for personalized diagnosis to precisely subtype rare malignancies such as MPM. PMID:27187383

  4. Systemic Control of Cell Division and Endoreduplication by NAA and BAP by Modulating CDKs in Root Tip Cells of Allium cepa

    Directory of Open Access Journals (Sweden)

    Jigna G. Tank

    2014-01-01

    Full Text Available Molecular mechanism regulated by auxin and cytokinin during endoreduplication, cell division, and elongation process is studied by using Allium cepa roots as a model system. The activity of CDK genes modulated by auxin and cytokinin during cell division, elongation, and endoreduplication process is explained in this research work. To study the significance of auxin and cytokinin in the management of cell division and endoreduplication process in plant meristematic cells at molecular level endoreduplication was developed in root tips of Allium cepa by giving colchicine treatment. There were inhibition of vegetative growth, formation of c-tumor at root tip, and development of endoreduplicated cells after colchicine treatment. This c-tumor was further treated with NAA and BAP to reinitiate vegetative growth in roots. BAP gave positive response in reinitiation of vegetative growth of roots from center of c-tumor. However, NAA gave negative response in reinitiation of vegetative growth of roots from c-tumor. Further, CDKs gene expression analysis from normal, endoreduplicated, and phytohormone (NAA or BAP treated root tip was done and remarkable changes in transcription level of CDK genes in normal, endoreduplicated, and phytohormones treated cells were observed.

  5. Concentrações de ANA e BAP na micropropagação de abacaxizeiro L. Merrill (Ananas comosus e no cultivo hidropônico das plântulas obtidas in vitro The effect of ANA and BAP concentrations on the micropropagation and hydroponic cultures of pineapple

    Directory of Open Access Journals (Sweden)

    Cristiane Elizabeth Costa de Macêdo

    2003-12-01

    Full Text Available O efeito de diferentes concentrações de ANA e BAP na micropropagação do abacaxizeiro, bem como no cultivo em hidroponia das plântulas obtidas in vitro, foram estudados em brotos de abacaxizeiro da variedade Pérola, inoculados em meio de cultura básico MS, suplementado com os fitorreguladores BAP e ANA em diferentes concentrações. Caracteres morfológicos quantitativos relacionados ao crescimento dos brotos e das plântulas de abacaxizeiro foram avaliados respectivamente durante os cultivos in vitro e em hidroponia e mostraram que, o tratamento T1 (BAP = 1,0 mg L-1e ANA = 0,5 mg L-1 proporcionou a maior taxa média de regeneração de brotos e conseqüentemente uma maior produção de matéria fresca. Entretanto, a altura dos brotos e a formação de suas raízes foram maiores nos tratamentos T2 (BAP = 0,5 mg L-1 e ANA = 0,25 mg L-1 e T3 (BAP = 0,25 mg L-1 e ANA = 0,12 mg L-1 . Após sessenta dias de cultivo em hidroponia, todas as plântulas oriundas do tratamento T1 apresentaram um bom desenvolvimento, expresso pela maioria dos caracteres morfológicos avaliados. O sistema de micropropagação utilizado neste trabalho possibilitou a obtenção de brotos de abacaxizeiro Pérola, em quantidade suficiente e ao mesmo tempo de fácil individualização, seguida da regeneração de plântulas que foram cultivadas em hidroponia.The effect of different ANA and BAP concentrations on in vitro and hydroponic cultures were studied. Pineapple shoots derived from Pérola explants variety were inoculated in MS media containing BAP and ANA in different concentrations. Growth parameters of shoots and plantlets were measured for in vitro and hydroponic cultures. Showed a highest multiplication rates of shoots and consequently highest fresh matter production were obtained with BAP and ANA at the concentrations of 1,0 and 0,5 mg L-1 respectively. However, the shoot length as well as the root number formed were higher in the T2 (0,5 de BAP + 0,25 de ANA

  6. C-terminal mutations destabilize SIL1/BAP and can cause Marinesco-Sjögren syndrome.

    Science.gov (United States)

    Howes, Jennifer; Shimizu, Yuichiro; Feige, Matthias J; Hendershot, Linda M

    2012-03-01

    Marinesco-Sjögren syndrome (MSS) is an autosomal recessive, neurodegenerative, multisystem disorder characterized by severe phenotypes developing in infancy. Recently, mutations in the endoplasmic reticulum (ER)-associated co-chaperone SIL1/BAP were identified to be the major cause of MSS. SIL1 acts as a nucleotide exchange factor for BiP, the ER Hsp70 orthologue, which plays an essential role in the folding and assembly of nascent polypeptide chains in the ER. SIL1 facilitates the release of BiP from unfolded protein substrates, enabling the subsequent folding and transport of the protein. Although most mutations leading to MSS result in deletion of the majority of the protein, three separate mutations have been identified that disrupt only the last five or six amino acids of the protein, which were assumed to encode a divergent ER retention motif. This study presents an in depth analysis of two of these mutants and reveals that the phenotype in the affected individuals is not likely to be due to depletion of SIL1 from the ER via secretion. Instead, our analyses show that the mutant proteins are particularly unstable and either form large aggregates in the ER or are rapidly degraded via the proteasome. In agreement with our findings, homology modeling suggests that the very C-terminal residues of SIL1 play a role in its structural integrity rather than its localization. These new insights might be a first step toward a possible pharmacological treatment of certain types of MSS by specifically stabilizing the mutant SIL1 protein.

  7. 肝豆状核变性致骨关节损害的临床观察及骨碱性磷酸酶(BAP)水平的研究%The Clinical Observe in Osteoarticular Damage on HLD and Relative Variation in BAP

    Institute of Scientific and Technical Information of China (English)

    李满棠; 罗成宏; 崔玉真

    2011-01-01

    Objective:To observe clinic manifastation,X-ray change,bone alkaline phosphatase(BAP) of osteoarticular damage on hepatolenticular degeneration, and analysis the variation of BAP after decopper therapy accompanied by calsium supplement and activated vitamin D.Methods: All 48 patients in experimental group,analysis the clinic manifastation,all 48 patients are examined by X-ray.20 volunteers in matched group.BAP in two groups are examined by ELISA.BAP are examined again after decopper therapy accompanied by calsium supplement and activated vitamin D for two month.The stasitcal data of two groups and before and after treatment is contastive analysised,.Results:The activity testing of BAP is more specific,sensitive,accurate to diagnose the osteoarticular damage on hepatolenticular degeneration.Conclusion: Decopper therapy accompanied by calsium supplement and activated vitamin D should be starts as early as possible according to the cause of osteoarticular damage on hepatolenticular degeneration,which can effectivly prevent the evolvement of osteoarticular danmage and relief the symptoms, improve the living quality of patients.%目的:对肝豆状核变性致骨关节损害的各种症状、体征、X线检查、骨碱性磷酸酶(BAP)水平进行观察,并对驱铜治疗及配合钙剂和活性维生素D制剂治疗后BAP水平进行统计研究.方法:实验组48例患者统计骨关节损害临床症状情况,全部行相应部位X片检查.另设20名健康志愿者为健康对照组.两组均用ELISA法检验BAP.于常规驱铜治疗加钙剂和活性维生素D制剂治疗,2个月后再次检验BAP,并进行治疗前后及与健康对照组的数值统计对比.结果:BAP 活性测定对HLD致骨关节损害诊断具有更好的特异性、灵敏性、准确性.结论:针对HLD骨关节病变的发病原因尽量早期采取有效驱铜治疗及配合钙剂和活性维生素D制剂治疗,可以有效预防骨关节损害进展和缓解症状,以改善HLD患者的生活质量.

  8. 降解苯并[a]芘的Bacillus pumilus strain Bap9菌株的分离、鉴定与降解特性%Isolation, Identification, and Biodegradation Characteristics of a Benzo [ a ] pyrene-degrading Bacterium Bacillus pumilus strain Bap9

    Institute of Scientific and Technical Information of China (English)

    朱婷婷; 倪晋仁

    2012-01-01

    A bacterial strain Bap9, which is able to utilize benzo [ a ] pyrene as sole carbon and energy source,was isolated from the polluted soil collected from the open-air barbecue market. The strain was identified as Bacillus pumilus according to its 16S rRNA sequence and physiological characteristic analysis. The experimental results showed that the strain could degrade 27. 3% of benzo[ a ] pyrene(40 mg/L) after a 20d incubation. The influences of pH,inoculum quantity and aeration on the cell growth and BaP degradation were examined, through the analysis of OD600 and the BaP concentrations. Strain Bap9 could grow and utilize benzo [ a ] pyrene at pH 6. 0- 9.0, and its optimal condition for the benzo [a] pyrene degradation was pH 8. 0, inoculum quantity 5% ,aeration 10ml/50ml.%从长期受烧烤影响的土壤中,分离筛选出一株能利用苯并[a]芘作为唯一碳源和能源的高效降解菌Bap9,通过16S rRNA基因序列分析和部分生理生化特征分析,鉴定为Bacillus pumilus.摇瓶试验结果表明,菌株Bap9能在20d内将40mg/L的苯并[a]芘降解27.3%.通过对OD600和苯并[a]芘残留浓度的检测分析,考察了pH值、接种量和装液量对菌株生长和降解性能的影响.结果表明,菌株在pH值为6.0-9.0的条件下均能生长并能降解苯并[a]芘,降解的最适pH值为8.0,最佳接种量为5%,最佳装液量为10ml/50ml.

  9. Glycolic acid inhibits enzymatic, hemorrhagic and edema-inducing activities of BaP1, a P-I metalloproteinase from Bothrops asper snake venom: insights from docking and molecular modeling.

    Science.gov (United States)

    Pereañez, Jaime Andrés; Patiño, Arley Camilo; Rey-Suarez, Paola; Núñez, Vitelbina; Henao Castañeda, Isabel Cristina; Rucavado, Alexandra

    2013-09-01

    Glycolic acid (GA) (2-Hydroxyethanoic acid) is widely used as chemical peeling agent in Dermatology and, more recently, as a therapeutic and cosmetic compound in the field of skin care and disease treatment. In this work we tested the inhibitory ability of glycolic acid on the enzymatic, hemorrhagic and edema-inducing activities of BaP1, a P-I metalloproteinase from Bothrops asper venom, which induces a variety of toxic actions. Glycolic acid inhibited the proteolytic activity of BaP1 on azocasein, with an IC₅₀ of 1.67 mM. The compound was also effective at inhibiting the hemorrhagic activity of BaP1 in skin and muscle in experiments involving preincubation of enzyme and inhibitor prior to injection. When BaP1 was injected i.m. and then, at the same site, different concentrations of glycolic acid were administered at either 0 or 5 min, 7 mM solutions of the inhibitor partially abrogated hemorrhagic activity when administered at 0 min. Moreover, glycolic acid inhibited, in a concentration-dependent manner, edema-forming activity of BaP1 in the footpad. In order to have insights on the mode of action of glycolic acid, UV-vis and intrinsic fluorescence studies were performed. Results of these assays suggest that glycolic acid interacts directly with BaP1 and chelates the Zn²⁺ ion at the active site. These findings were supported by molecular docking results, which suggested that glycolic acid forms hydrogen bonds with residues Glu143, Arg110 and Ala111 of the enzyme. Additionally, molecular modeling results suggest that the inhibitor chelates Zn²⁺, with a distance of 3.58 Å, and may occupy part of substrate binding cleft of BaP1. Our results suggest that glycolic acid is a candidate for the development of inhibitors to be used in snakebite envenomation.

  10. The random amplified polymorphic DNA (RAPD) assay to determine DNA alterations, repair and transgenerational effects in B(a)P exposed Daphnia magna.

    Science.gov (United States)

    Atienzar, Franck A; Jha, Awadhesh N

    2004-08-18

    The random amplified polymorphic DNA (RAPD) is a useful assay for the detection of genotoxin-induced DNA damage and mutations. In this study, we have further evaluated the potential of this assay to measure benzo(a)pyrene [B(a)P]-induced DNA changes, and repair (in kinetic experiments) as well as transgenerational effects in the water fleas, Daphnia magna. The organisms, which reproduce parthenogenetically, were exposed to 50 microg L(-1) B(a)P for 3 or 6 days and were allowed to recover in clean medium for 12 or 9 days, respectively. Qualitative and quantitative changes were observed in RAPD profiles generated not only from the B(a)P exposed Daphnia but also from previously treated organisms during the recovery experiments. The fact that some of the RAPD changes disappeared at the end of both recovery experiments suggested that the DNA effects were fully repaired or reversed. In addition, some of the B(a)P-induced RAPD alterations detected in parental D. magna were also observed in the offspring patterns. This suggested that DNA alterations that occurred in germ cells were probably transmitted to the next cohorts. The present study shows that the RAPD method can be useful to qualitatively assess the kinetics of DNA changes, repair and transgenerational effects and such effects could potentially be linked to survival and reproductive success at higher levels of biological organisation. In addition, the water fleas have efficient capabilities to repair or reverse B(a)P-induced DNA effects. Finally, unrepaired or misrepaired genetic damage induced by genotoxins such as B(a)P could be transmitted to next generations in these parthenogenetically reproducing organisms. PMID:15288546

  11. Development of a reliable assay protocol for identification of diseases (RAPID)-bioactive amplification with probing (BAP) for detection of Newcastle disease virus.

    Science.gov (United States)

    Wang, Chi-Young; Hsu, Chia-Jen; Chen, Heng-Ju; Chulu, Julius L C; Liu, Hung-Jen

    2008-07-27

    Due to appearance of new genotypes of Newcastle disease virus (NDV) with no cross-protection and with vaccine strains, some outbreaks have been reported in Taiwan that caused significant damage to the poultry industry. A reliable assay protocol, (RAPID)-bioactive amplification with probing (BAP), for detection of NDV that uses a nested PCR and magnetic bead-based probe to increase sensitivity and specificity, was developed. Primers and probes were designed based on the conserved region of the F protein-encoding gene sequences of all NDV Taiwan isolates. The optimal annealing temperature for nested reverse transcription-polymerase chain reaction (RT-PCR) to amplify the gene was 61 degrees C and optimal hybridization occurred when buffer 1x SSC and 0.5% SDS were used at 50 degrees C. The sensitivity of RAPID-BAP was 1 copy/microl for standard plasmids and 10 copy/mul for transcribed F protein-encoding gene of NDV with comparable linearity (R(2)=0.984 versus R(2)=0.99). This sensitivity was superior to that of other techniques currently used. The assay was also highly specific because the negative controls, including classical swine fever virus, avian influenza virus, avian reovirus, and infectious bursa disease virus could not be detected. Thirty-four field samples were tested using conventional RT-PCR, nested RT-PCR, real-time quantitative RT-PCR, and RAPID-BAP assay and the positive rates were 24%, 30%, 41%, and 53%, respectively. The developed assay allows for rapid, correct, and sensitive detection of NDV and fulfils all of the key requirements for clinical applicability. It could reliably rule out false negative results from antibody-based assays and also facilitate a rapid diagnosis in the early phase of the disease for emergency quarantine that may help prevent large-scale outbreaks.

  12. Correlation of the Levels of the Bone Turnover Markers BAP and β-CTX with Bone Metastasis Progress in Lung Cancer Patients

    OpenAIRE

    Tang, Qiong; Zhao, Hui; JIA, RUI; Liu, Linlin

    2013-01-01

    Background and objective Bone metastasis is common in lung cancer patients. The β isomer of the C-terminal telopeptide of type I collagen (β-CTX) and bone-specific alkaline phosphates (BAP) are regarded as important bone turnover markers in bone resorption and formation. Thus, the aims of this study are to determine the correlation of these bone turnover markers with the extent of bone metastasis of lung cancer. Methods A total of 92 patients with bone metastasis of lung cancer from Tianjin U...

  13. 不同碳源对苯并[a]芘降解菌生长和降解性能影响的研究%Effect of Different Kinds of Extra Carbon Sources on the Biomass Production and BaP Removal by Bacillus pumilus Strain Bap9

    Institute of Scientific and Technical Information of China (English)

    朱婷婷; 倪晋仁

    2012-01-01

    采用从受户外烧烤影响的土壤中分离出的苯并[a]芘降解菌Bacillus pumilus strain Bap9,利用摇床实验研究了不同外加碳源对其生长和苯并[a]芘降解性能的影响。结果表明,苯并[a]芘初始浓度为40mg/L时,蔗糖、葡萄糖、麦芽糖的存在会抑制菌株对苯并[a]芘的降解,可溶性淀粉的影响不明显。以醋酸钠为外加碳源可明显促进菌株的降解作用,添加40mg/L的醋酸钠,20天后可将菌株Bap9的降解率提高了8.8%,但过量的碳源会抑制菌株对苯并[a]芘的降解。添加适量的低分子量菲(PAXH)作为共代谢底物,菲的促进作用大于同浓度的醋酸钠,添加40mg/L的菲,20天后菌株对苯并[a]芘的降解率提高了26.4%。%Batch experiments were conducted in order to investigate the effects of extra carbon sources on benzo[a]pyrene (BaP) biodegradation by Bacillus pumilus strain Bap9, which was isolated from polluted soil collected from open-air barbecue market. In the culture system under initial BaP concentration of 40 mg/L, the addition of sucrose, glucose, maltose reduced the degradation rates, the presence of soluble starch had no obvious effect on the biodegradation, while the supplement of sodium acetate increased by 8.8% of the degradation rates most effectively. However, if the concentrations were too high, sodium acetate would show the inhibition of biodegradation. The supplementation of phenanthrene (Phe), was more effective on increasing the degradation rate than sodium acetate of the same concentration. The biodegradation rate of BaP increased 26.4% after a 20 days incubation period, while Phe of 40 mg/L was used as the co-metabolism substrate.

  14. BAP1 missense mutation c.2054 A>T (p.E685V completely disrupts normal splicing through creation of a novel 5' splice site in a human mesothelioma cell line.

    Directory of Open Access Journals (Sweden)

    Arianne Morrison

    Full Text Available BAP1 is a tumor suppressor gene that is lost or deleted in diverse cancers, including uveal mela¬noma, malignant pleural mesothelioma (MPM, clear cell renal carcinoma, and cholangiocarcinoma. Recently, BAP1 germline mutations have been reported in families with combinations of these same cancers. A particular challenge for mutation screening is the classification of non-truncating BAP1 sequence variants because it is not known whether these subtle changes can affect the protein function sufficiently to predispose to cancer development. Here we report mRNA splicing analysis on a homozygous substitution mutation, BAP1 c. 2054 A&T (p.Glu685Val, identified in an MPM cell line derived from a mesothelioma patient. The mutation occurred at the 3rd nucleotide from the 3' end of exon 16. RT-PCR, cloning and subsequent sequencing revealed several aberrant splicing products not observed in the controls: 1 a 4 bp deletion at the end of exon 16 in all clones derived from the major splicing product. The BAP1 c. 2054 A&T mutation introduced a new 5' splice site (GU, which resulted in the deletion of 4 base pairs and presumably protein truncation; 2 a variety of alternative splicing products that led to retention of different introns: introns 14-16; introns 15-16; intron 14 and intron 16; 3 partial intron 14 and 15 retentions caused by activation of alternative 3' splice acceptor sites (AG in the introns. Taken together, we were unable to detect any correctly spliced mRNA transcripts in this cell line. These results suggest that aberrant splicing caused by this mutation is quite efficient as it completely abolishes normal splicing through creation of a novel 5' splice site and activation of cryptic splice sites. These data support the conclusion that BAP1 c.2054 A&T (p.E685V variant is a pathogenic mutation and contributes to MPM through disruption of normal splicing.

  15. Influência da benzilaminopurina (BAP NA multiplicação in vitro de kiwi (Actinidia deliciosa The influence of benzylaminopurine in the in vitro multiplication of kiwi (Actinidia deliciosa

    Directory of Open Access Journals (Sweden)

    Jair Costa Nachtigal

    1995-01-01

    Full Text Available Este experimento foi conduzido no Laboratório de Cultura de Tecidos da EMBRAPA/CPACT, Pelotas, RS, com o objetivo de estudar a influência de diferentes concentrações de 6-benzilaminopurina (BAP na multiplicação in vitro de kiwi (Actinidia deliciosa, cv. Tomun. Utilizou-se o meio de cultura MS, acrescido de sacarose, mio-inositol, ágar e das seguintes concentrações de BAP: 0,0; 0,5; 1,0; 1,5; 2,0 e 2,5 mg/l. Os explantes foram constituídos de microestacas provenientes da coleção in vitro do Laboratório, com aproximadamente 10mm de comprimento. Verificou-se que, para o número e comprimento de brotações, número de gemas e folhas a concentração em torno de 1,5mg/l de BAP proporcionou os memores resultados. Nas condições em que o trabalho foi realizado, pode-se concluir que o BAP foi eficiente na multiplicação in vitro de kiwi, cv. Tomuri.This trial was carried out in the Tissue Culture Laboratory at EMBRAPA/CPACT, Pelotas, RS, Brazil, aiming to study the influence of different 6-benzylaminopurine (BAP concentrations in the in vitro multiplication of kiwi, cv. Tomuri. A MS medium was used adding sucrose, myo-inositol, agar and concentrations of BAP as follows: 0.0; 0.5; 1.0; 1.5; 2.0 and 2.5mg/l. The explants consisting of microshoots of 10mm length were obtained from the in vitro collection at the laboratory. The best results of bud and shoot numbers, shoot length and number of leaves were obtained using about 1.5mg/l BAP. Results of this experiment demonstrated that BAP is efficient in the in vitro multiplication of kiwi, cv. Tomuri.

  16. Indução de calos em sempre-viva (Syngonanthus mucugensis Giulietti, utilizando diferentes tipos de explantes e concentrações de BAP = Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti using different types of explants and BAP concentrations

    Directory of Open Access Journals (Sweden)

    Janilza da Paixão Santos

    2008-04-01

    Full Text Available A sempre-viva-de-mucugê [Syngonanthus mucugensis – Eriocaulaceae] é uma planta com grande valor ornamental e caracteriza-se pela durabilidade de suas inflorescências que se mantém mesmo depois de coletadas e secas. A propagação sexuada dessa espécie resulta em plantas desuniformes por ser geneticamente segregante e, aliada aeste fato, existe a ameaça de extinção da espécie, sendo por isso restrita a coleta na sua região de origem. Desta forma, a cultura de tecidos torna-se uma alternativa viável para a formação de novas mudas e, por isso, objetivou-se, neste trabalho, avaliar a indução de calos in vitro de Syngonanthus mucugensis, utilizando diferentes concentrações de BAP (6-benzilaminapurina. O delineamento experimental foi inteiramente casualizado (DIC, sendo que cadatratamento continha quatro repetições e cada repetição era formada por quatro explantes. Os explantes utilizados foram plantas cultivadas in vitro e segmentos nodais delas. O meio de cultivo utilizado foi o MS (metade dos sais, suplementado com (0,0; 0,89; 1,78; 3,55; 7,10; 14,21 e 28,42 μM de BAP. Aos 60 dias, avaliaram-se a sobrevivência dos explantes e a porcentagem de formação de calos. Verificou-se que plantas inteiras e segmentos nodais deSyngonanthus mucugensis são explantes responsíveis à formação de calos friáveis, sendo que a produção significativa é obtida utilizando-se as concentrações de 1,78 e 3,55 μM de BAP.Sempre-viva-de-mucugê [Syngonanthus mucugensis – Eriocaulaceae] is a plant with great ornamental value characterized by the durability of its inflorescences, which remains even after collected and dried. Thesexual propagation of this species results in disuniform plants, as it is genetically segregating; in addition, this species is currently endangered, with collection therefore restricted to its region of origin. Thus, tissue culture becomes a viable alternative for theformation of new plants. This study aimed to

  17. Biofilm production and presence of ica and bap genes in Staphylococcus aureus strains isolated from cows with mastitis in the eastern Poland.

    Science.gov (United States)

    Szweda, Piotr; Schielmann, Marta; Milewski, Sławomir; Frankowska, Aneta; Jakubczak, Antoni

    2012-01-01

    The aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested using two phenotypic methods. The Congo Red plate assay was found to be irreproducible and very subjective. More objective results were obtained using the spectrophotometric, microtiter plate assay. Most of the isolates, namely 76/132 (57.6 %) were classified as biofilm producers depending on the value of absorbance in the microtiter plate test. All of the isolates tested were found to possess both icaA and icaD genes, while the bap gene was absent in all strains.

  18. Enhancement of hypoxia-induced gene expression in fish liver by the aryl hydrocarbon receptor (AhR) ligand, benzo[a]pyrene (BaP).

    Science.gov (United States)

    Yu, Richard Man Kit; Ng, Patrick Kwok Shing; Tan, Tianfeng; Chu, Daniel Ling Ho; Wu, Rudolf Shiu Sun; Kong, Richard Yuen Chong

    2008-11-21

    Fish in polluted coastal habitats commonly suffer simultaneous exposure to both hypoxia and xenobiotics. Although the adaptive molecular responses to each stress have been described, little is known about the interaction between the signaling pathways mediating these responses. Previous studies in mammalian hepatoma cell lines have shown that hypoxia-inducible factor (HIF)- and/or aryl hydrocarbon receptor (AhR)-activated gene expression is suppressed following co-exposure to hypoxia and the hallmark AhR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). However, whether similar crosstalk exists in the non-tumor liver tissues of fish and whether other non-TCDD ligands also play the same inhibitory role in this crosstalk remain unknown. Here, the in vivo hepatic mRNA expression profiles of multiple hypoxia- and AhR-responsive genes (later gene expression=mRNA expression of the gene) were examined in the orange-spotted grouper (Epinephelus coioides) upon single and combined exposures to hypoxia and benzo[a]pyrene (BaP). Combined exposure enhanced hypoxia-induced gene expression but did not significantly alter BaP-induced gene expression. Protein carbonyl content was markedly elevated in fish subjected to combined exposure, indicating accumulation of reactive oxygen species (ROS). Application of diethyldithiocarbamate (DDC) to hypoxia-treated grouper liver explants similarly exaggerated hypoxia-induced gene expression as in the combined stress tissues in vivo. These observations suggest that ROS derived from the combined hypoxia and BaP stress have a role in enhancing hypoxia-induced gene expression.

  19. Performance assessment of a nested-PCR assay (the RAPID BAP-MTB) and the BD ProbeTec ET system for detection of Mycobacterium tuberculosis in clinical specimens.

    Science.gov (United States)

    Wang, Jann-Yuan; Lee, Li-Na; Chou, Chin-Sheng; Huang, Chung-Yi; Wang, Shu-Kuan; Lai, Hsin-Chih; Hsueh, Po-Ren; Luh, Kwen-Tay

    2004-10-01

    The performance of a nested PCR-based assay (the RAPID BAP-MTB; AsiaGen, Taichung, Taiwan) and the BD ProbeTec ET (DTB) system (Becton Dickinson, Sparks, Md.) for detection of Mycobacterium tuberculosis was evaluated with 600 consecutive clinical samples. These samples, including 552 respiratory specimens and 48 nonrespiratory specimens, were collected from 333 patients treated at National Taiwan University Hospital from September to October 2003. The results of both assays were compared to the gold standard of combined culture results and clinical diagnosis. The overall sensitivity and specificity of the RAPID BAP-MTB assay for respiratory specimens were 66.7% and 97.2%, respectively, and for the DTB assay they were 56.7% and 95.3%, respectively. The positive and negative predictive values for the RAPID BAP-MTB were 74.1% and 96.0%, respectively, and for the DTB assay they were 59.6% and 94.7%, respectively. For smear-negative samples, the sensitivity of the RAPID BAP-MTB and DTB assays was 57.1% and 40.5%, respectively. The RAPID BAP-MTB assay produced 14 false-positive results in 14 samples, including one of the six samples yielding Mycobacterium abscessus, one of the six samples yielding Mycobacterium avium intracellulare, one sample from a patient with a history of pulmonary tuberculosis with complete treatment, and three samples from three patients with a previous diagnosis of tuberculosis who were under treatment at the time of specimen collection. Among the 48 nonrespiratory specimens, the RAPID BAP-MTB assay was positive in one biopsy sample from a patient with lumbar tuberculous spondylitis and one pus sample from a patient with tuberculous cervical lymphadenopathy. Our results showed that the RAPID BAP-MTB assay is better than the DTB assay for both respiratory specimens and nonrespiratory specimens. The overall time for processing this assay is only 5 h. In addition, its diagnostic accuracy in smear-negative samples is as high as in smear

  20. 最佳水产养殖规范(BAP)认证及其在中国的发展%Best Aquaculture Practice certificate and its possible impact on international trade of Chinese seafood

    Institute of Scientific and Technical Information of China (English)

    刘利平; 赵广学; 张宗峰

    2011-01-01

    最佳水产养殖规范(BAP)秉承可持续发展的理念,被国际认证组织作为一种新的认证标准走进水产业并逐渐受到人们的重视.该认证主要针对一些水产养殖企业或基地的重要水产养殖品种的育苗、养殖和食品加工以及流通等过程,按照特定的标准对企业进行认证.目前已经形成了对虾、罗非鱼、斑点叉尾鮰、越南鲶鱼和鲑鱼的标准.本文主要以凡纳滨对虾和罗非鱼产业为例,介绍BAP和BAP认证的内容、认证程序、在中国和世界的发展趋势,以及对我国水产养殖业可能产生的影响.%With the prosperous development of world aquaculture together with the significance of certificates and standards on aquaculture industry, "Best Aquaculture Practice, BAP" was raised and well known to aquaculturists with its core idea of sustaining development. As a NGO, Global Aquaculture Alliance (GAA)has been framing and advocating the "best aquaculture practice" certificate, which aims at the hatcheries,farms, processors, feed mills and repacking plants worldwide for several commercially important species. Up to now, BAP standards for shrimp, tilapia, catfish, pangasius and salmon have been published online.Hundreds of plants in the world and dozens of plants in China have been issued with the Best Aquaculture Practice Certificate. However, BAP to be advocated as international standards of seafood may become a new trade barrier especially for small enterprises. In this paper, the origin of BAP, BAP certificate as well as its possible impacts on aquaculture in China has been presented taking shrimp and tilapia industry as examples.

  1. Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus, an Aquatic Edible Plant

    Directory of Open Access Journals (Sweden)

    Noraini Mahmad

    2014-01-01

    Full Text Available In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux. The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions.

  2. Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of Nelumbo nucifera Gaertn. (lotus), an aquatic edible plant.

    Science.gov (United States)

    Mahmad, Noraini; Taha, Rosna Mat; Othman, Rashidi; Saleh, Azani; Hasbullah, Nor Azlina; Elias, Hashimah

    2014-01-01

    In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions.

  3. Efeito do ácido naftaleno acético (ANA e benzilaminopurina (BAP no aumento do ângulo de inserção dos ramos em macieira The effect of naftalen acetic acid (NAA and benzilaminopurin (BAP in the increase of the angle of insertion of the branches in apple trees

    Directory of Open Access Journals (Sweden)

    Aike Anneliese Kretzschmar

    2004-08-01

    Full Text Available Objetivou-se avaliar a influência dos fitorreguladores ANA (ácido naftaleno acético e BAP (benzilaminopurina, no aumento do ângulo de inserção de ramos jovens em macieira. O experimento foi conduzido em um pomar comercial da região de Lages-SC, utilizando-se das cultivares Imperial Gala e Fuji, sobre porta-enxerto Marubakaido, testando diferentes doses de fitorreguladores e o arqueamento convencional. Os fitorreguladores foram aplicados com auxílio de algodão, na inserção entre os ramos e o caule, na parte superior dos ramos em crescimento. Os resultados evidenciaram que ANA teve um efeito positivo no arqueamento de ramos, nas doses de 200 a 500 ppm, mas a prática convencional para aumentar o ângulo de inserção dos ramos, através do uso de palitos, continua sendo o melhor método de arqueamento.The objective of this experiment was to evaluate the influence of the growth regulators NAA (naftalen acetic acid and BAP (benzilaminopurin, in the increase of the angle of insertion of the branches in apple trees. The experiment was driven in a commercial orchard area of Lages/SC, where has been cultivated Imperial Gala and Fuji cultivars on rootstock Marubakaido, testing different doses of the regulators compared with the conventional bending. The regulators were applied with cotton aid, in the superior part of the branches in growth, in the insertion between the branch and the stem. The results evidenced that, even NAA tends to have a good effect in the bending of branches, in the doses of 200 to 500 ppm, the conventional practice to increase the angle of insertion of the branches, through the use of toothpicks, continues being the best bending method.

  4. Increase of carcinogenic risk via enhancement of cyclooxygenase-2 expression and hydroxyestradiol accumulation in human lung cells as a result of interaction between BaP and 17-beta estradiol.

    Science.gov (United States)

    Chang, Louis W; Chang, Yun-Ching; Ho, Chia-Chi; Tsai, Ming-Hsien; Lin, Pinpin

    2007-07-01

    Animal studies demonstrated that females are more susceptible than males to benzo[a]pyrene (BaP)-induced toxicities, including lung carcinogenesis. Elevation of cyclooxygenase-2 (COX-2) expression has been shown to increase the risk of cancer development. BaP induces COX-2 expression, and an interaction between BaP and estrogen in relation to COX-2 expression is suspected. In the present study, 10 muM BaP alone only slightly increased COX-2 mRNA expression and 10 nM 17-beta estradiol (E(2)) alone slightly increased prostaglandin E2 (PGE2) secretion in human bronchial epithelial cells. However, co-treatment with BaP and E(2) potentiated COX-2 mRNA expression and significantly elevated PGE2 secretion. Utilizing specific inhibitors and reporter assays, we further investigated the potentiation mechanisms of E(2) on BaP-induced COX-2 expression. First, E(2) activated estrogen receptor to increase PGE2 secretion, which directly increased COX-2 expression. Second, E(2) potentiated BaP-induced nuclear factor-kappaB (NF-kappaB) activation, which regulates COX-2 expression. Third, although the aryl hydrocarbon receptor (AhR) did not play a role in BaP-induced COX-2 expression, the potentiation effect of E(2) itself was AhR dependent. We further demonstrated that BaP induced the production of genotoxic E(2) metabolites (2- and 4-hydroxyestradiols) via AhR-up-regulated cytochromes P450 1A1 and 1B1. These metabolites could directly activate NF-kappaB to further promote COX-2 mRNA expression in human lung epithelial cells. These findings were further supported by increased PGE2 secretion in rat lung slice cultures. Our findings that the BaP-E(2) interaction enhanced COX-2 expression and hydroxyestradiol accumulation in the media of cultivated lung cells and tissues provide the needed scientific basis for higher risk of BaP-associated lung cancer in females.

  5. 大连湾沉积物-水界面BAP交换通量的影响因素%The influencing factors of BAP exchange flux at the sediment-water interface in Dalian Bay

    Institute of Scientific and Technical Information of China (English)

    宗虎民; 张志锋; 王燕; 张哲

    2012-01-01

    The influencing factors of BAP (bio-available phosphorus) exchange flux at the sediment-water interface in Dalian Bay were studied by laboratory incubation experiment, and the BAP fluxes between sediment and water in the dredged area and in the area near the sediment dredging location were analyzed with the methods of laboratory incubation experiment and nutrient concentration gradients in the pore water. The results showed that the fluxes of BAP from sediment to water increased with the increase of pH and temperature in overlying water, and it also increased by the condition of applying sterilized sediment and aerating in overlying water during the incubation experiment. The fluxes from sediment to water decreased when the concentrations of phosphorus increased in the overlying water, and the flux direction reversed with the continuous increase of the concentrations. Both results of the two methods showed that the annual average exchange flux of BAP between sediment and water in the area near the sediment dredging location was higher than that in dredged area affected by dredging works. The exchange fluxes of BAP between sediment and water in Dalian Bay were different in four seasons, which was highest in summer and lowest in winter. However, it was at a high level in the area near the sediment dredging location in winter due to the impact of dredging.%利用实验室培养法研究了不同因素对大连湾沉积物-水界面间生物可利用磷(BAP)交换通量的影响,并利用实验室培养法和间隙水浓度梯度法估算了大连湾已疏浚区和疏浚点邻近海域沉积物-水界面BAP的交换通量.结果表明,随着上覆水pH和温度的增加,BAP由沉积物向水体释放量逐渐增加;灭菌和贫氧条件下,也会导致沉积物BAP的释放量增加;上覆水中磷酸盐浓度的升高会导致BAP由沉积物向水体释放通量降低,且随着上覆水磷酸盐浓度的不断升高,BAP逐渐转化为由水体向沉积物中扩散.

  6. Correlation of the Levels of the Bone Turnover Markers BAP and β-CTX with Bone Metastasis Progress in Lung Cancer Patients%BAP和β-CTX与肺癌骨转移进展程度的相关性

    Institute of Scientific and Technical Information of China (English)

    唐琼; 赵辉; 贾锐; 刘林林

    2013-01-01

    背景与目的 肺癌易发生骨转移,Ⅰ型胶原羧基端肽β特殊序列(β isomer of C-terminal telopeptide of type Ⅰ collagen,β-CTX)和骨源性碱性磷酸酶(bone-specific alkaline phosphates,BAP)在骨质代谢中是重要的生化标记物,本研究旨在探讨骨代谢生化指标和肺癌骨转移进展程度的相关性,有助于早期判断是否有肺癌骨转移的发生.方法 天津市人民医院2009年7月-2012年7月共收治92例肺癌并发生骨转移患者,全部病例其原发灶均经细胞学检查证实,骨转移灶的数目及类型经ECT结合X线片、CT证实,采用ELISA方法检测血β-CTX、BAP的浓度水平.结果 收集的92例肺癌伴骨转移的患者中,转移灶<3处为58例,≥3处为34例;溶骨型68例,成骨型9例,混合型15例.血β-CTX、BAP水平在不同的骨转移数目组间差异有统计学意义(P<0.05),β-CTX在肺癌溶骨型骨转移患者中具有较高的敏感性.结论 骨代谢生化指标β-CTX、BAP对肺癌骨转移具有辅助诊断意义,是判断转移进展程度的良好指标.%Background and objective Bone metastasis is common in lung cancer patients. The β isomer of the C-terminal telopeptide of type Ⅰ collagen (β-CTX) and bone-specific alkaline phosphates (BAP) are regarded as important bone turnover markers in bone resorption and formation. Thus, the aims of this study are to determine the correlation of these bone turnover markers with the extent of bone metastasis of lung cancer. Methods A total of 92 patients with bone metastasis of lung cancer from Tianjin Union Medicine Center were enrolled in this study between July 2009 and July 2012. The bone metastasis lesion was diagnosed by ECT combined with X-ray and CT imaging. Trie bone turnover marker levels of β-CTX and BAP were measured by ELISA. Results Among the 92 patients, 58 cases had 3; 68 cases had osteolytic bone metastasis, 9 had osteoblastic bone metastasis, and 15 had mixed bone metastases. The serum levels

  7. The Bath Adolescent Pain--Parental Impact Questionnaire (BAP-PIQ): development and preliminary psychometric evaluation of an instrument to assess the impact of parenting an adolescent with chronic pain.

    Science.gov (United States)

    Jordan, Abbie; Eccleston, Christopher; McCracken, Lance M; Connell, Hannah; Clinch, Jacqui

    2008-07-31

    When an adolescent has chronic pain many aspects of a parent's life can be affected, including their emotional and social functioning. The assessment of this multidimensional parental impact is an essential, yet often neglected, clinical task. This study reports on the development and psychometric evaluation of the Bath Adolescent Pain--Parental Impact Questionnaire (BAP-PIQ), an assessment tool comprising multiple scales thought to be relevant for better understanding changes in functioning and behavior associated with parenting an adolescent with chronic pain. A sample of 194 parents of adolescents with chronic pain, recruited from three UK clinics, completed the 94 item draft inventory. Frequency and item correlation analyses resulted in a final inventory of 62 items. Internal consistency of all eight scales was established based on Cronbach's alpha. Convergent validity was undertaken by comparison of individual scales with existing validated measures of parental stress, mood, parenting behavior, marital adjustment, and general functioning. The temporal reliability of each scale was established using a sub-sample of 46 participants over a 14-day period. Psychometric evaluation suggests that the inventory yields a reliable and valid assessment of the multiple impacts of parenting an adolescent with chronic pain. The BAP-PIQ may offer a comprehensive assessment of these impacts in both a research and a clinical setting. Further study of the validity of BAP-PIQ scales and their ability to detect clinically meaningful change would be of use. Additional data from samples comprising fathers of adolescents with chronic pain and parents of adolescents with non-musculoskeletal pain would be of benefit.

  8. Genotoxicity assessment in Eisenia andrei coelomocytes: a study of the induction of DNA damage and micronuclei in earthworms exposed to B[a]P- and TCDD-spiked soils.

    Science.gov (United States)

    Sforzini, Susanna; Boeri, Marta; Dagnino, Alessandro; Oliveri, Laura; Bolognesi, Claudia; Viarengo, Aldo

    2012-07-01

    Earthworms are useful indicators of soil quality and are widely used as model organisms in terrestrial ecotoxicology. The assessment of genotoxic effects caused by environmental pollutants is of great concern because of their relevance in carcinogenesis. In this work, the earthworm Eisenia andrei was exposed for 10 and 28 days to artificial standard soil contaminated with environmentally relevant concentrations of benzo[a]pyrene (B[a]P) (0.1, 10, 50ppm) and 2,3,7,8-tetrachloro-dibenzo-para-dioxin (TCDD) (1×10(-5), 1×10(-4), 2×10(-3)ppm). Micronucleus (MNi) induction was evaluated in earthworm coelomocytes after DNA staining with the fluorescent dye DAPI. In the same cells, the DNA damage was assessed by means of the alkaline comet assay. Induction of MNi in coelomocytes, identified according to standard criteria, was demonstrated. B[a]P exposure for 10 and 28 days induced a significant increase in MNi frequency. In TCDD-treated earthworms, a significant effect on chromosomal damage was observed at all the concentrations used; surprisingly, greater effects were induced in animals exposed to the lowest concentration (1×10(-5)ppm). The data of the comet assay revealed a significant increase in the level of DNA damage in coelomocytes of earthworms exposed for 10 and 28 days to the different concentrations of B[a]P and TCDD. The results show that the comet and MN assays were able to reveal genotoxic effects in earthworms exposed even to the lowest concentrations of both chemicals tested here. The combined application in E. andrei of the comet assay and the micronucleus test, which reflect different biological mechanisms, may be suggested to identify genotoxic effects induced in these invertebrates by environmental contaminants in terrestrial ecosystems. PMID:22459015

  9. Effect of insulin combined alendronate sodium on bone mineral density and levels of serum BAP, TRAP-5b and BGP in aged patients with type 2 diabetes mellitus with osteoporosis

    Institute of Scientific and Technical Information of China (English)

    Fang Wang; Han-Qiang Ren; Xiao-Bo Shen

    2016-01-01

    Objective:To explore the effect of insulin combined alendronate sodium on bone mineral density and levels of serum BAP, TRAP-5b and BGP in aged patients with type 2 diabetes mellitus with osteoporosis.Methods: A total of 136 patients with type 2 diabetes mellitus with osteoporosis in January 2014 to January 2016 in our hospital for the treatment were selected, and randomly divided into 4 groups, each of 40 cases. Caltrate D was given as a basic treatment to all the patients, and the control group was given the treatment of insulin, and the metformin group was given the treatment of metformin, and the combination group was given the treatment of metformin combined alendronate, and the experiment group was given the treatment of insulin combined alendronate. BMD of the femoral neck and the serum levels of BAP, TRAP-5b and BGP were detected and recorded before the treatment and after one year’s treatment.Results:On index of bone mineral density, the control group and the metformin group showed no significant differences; the combination group was slightly improved, but showed no statistical significance; After the treatment, the bone mineral density of the experiment was significantly improved. On index of bone turnover, the levels of serum BAP and BGP all had been improved and the level of TRAP-5b all was reduced then before the treatment in the control group, the combination group and the experiment group, but only the experiment group showed significant differences; On index of bone turnover, the experiment group were better than other groups, the differences were statistical significant.Conclusions:It has greater clinical curative effect that insulin combined alendronate sodium in the treatment of aged patients with type 2 diabetes mellitus with osteoporosis, it can effectively balance the metabolism of bone, safe and reliable, and it is worthy of application.

  10. Comparação dos efeitos da mistura de poluentes orgânicos (BAP, DDT e TBT) no metabolismo hepático e na toxicidade em teleósteos de água doce

    OpenAIRE

    Oliveira, Heloisa Helena Paro de

    2013-01-01

    Resumo: O presente estudo teve enfoque nos efeitos da mistura de três poluentes orgânicos persistentes (POPs) em peixes de água doce: benzo(a)pireno (BaP), um produto da combustão incompleta de materiais orgânicos e conhecido como carcinógeno humano; diclorodifeniltricloroetano (DDT), um pesticida organoclorado persistente que causa danos à vida selvagem e também pode ter efeitos negativos na saúde humana; e tributilestanho (TBT), usado em tintas anti-incrustantes e um dos agentes antropogêni...

  11. Analysis of Pollution Current Situation about Bap in the Surrounding Air in Tongling City%铜陵市环境空气苯并(a)芘污染现状分析

    Institute of Scientific and Technical Information of China (English)

    梅建鸣

    2005-01-01

    本文对铜陵市空气中苯并(a)芘(Bap)的污染水平及污染源进行了简要分析,指出焦化作业是造成空气BaP污染的重要污染源之一,对原焦化厂进行污染综合治理,实施移址建设,对降低城区BaP空气污染意义重大.

  12. 肺癌患者血清NTx、ICTP和BAP水平与骨转移的相关性研究%Relationships between the levels of sermn NTx, ICTP, BAP and bone metastasis in patients with lung cancer

    Institute of Scientific and Technical Information of China (English)

    张世强; 陈冬波; 王保庆; 张兰胜

    2011-01-01

    Objective To study the clinical significance of serum N-telopeptide of type I collagen( NTx), carboxy-terminal collagen telopeptide( ICTP), and bone-specific alkaline phosphatase(BAP) in diagnosing bone metastasis of lung cancer. Methods A total of 106 patients with lung cancer were diagnosed by pathology in this study. Patients were divided into two groups: 61 patients with bone metastasis and 45 patients without bone metastasis. The levels of serum NTx, ICTP and BAP were measured by ELISA. Results The levels of serum NTx, ICTP and BAP in bone metastasis group were (25.36 ± 11.07) nmol/L, (29. 18 ± 10. 74) μg/L and (78. 31 ± 16. 53 ) μg/L higher than ( 12. 16 ± 7.62) nmol/L, ( 11.02 ± 5.32)μg/L and (24. 01 ± 7.98 ) μg/L in non-bone metastasis group( P < 0. 01 ). The sensitivity and specificity of serum NTx in the diagnosis of bone metastasis were 90. 16% and 84. 44%, respectively. The sensitivity and specificity of serum BAP were 80. 32% and 77.78%, respectively. The sensitivity and specificity of serum ICTP were 70.49% and 91.11%, respectively. While The levels of serum NTx, ICTP and BAP in patients with more bone metastasis were significantly higher than those in single bone metastasis (P < 0. 05 ). The levels of serum NTx, ICTP and BAP were related to the degree of pain with bone metastasis. But we could not find that there were difference of serum NTx, ICTP and BAP among sex, pathology and different bone metastasis position. Conclusion The sensitivity and specificity of the serum NTx, ICTP and BAP are higher in diagnosis of lung cancer with bone metastasis. The three markers can be regarded as important factors for bone metastasis of lung cancer.%目的 探讨联合检测骨代谢生化指标血清Ⅰ型胶原氨基末端肽(NTx)、Ⅰ型胶原羧基端肽(ICTP)及骨特异性碱性磷酸酶(BAP)对诊断肺癌骨转移的临床意义.方法 选择经细胞学和病理学确诊的肺癌患者106例,分为两组,其中骨转移组61

  13. Effect of gibberellic acid (GA), indole acetic acid (IAA) and benzylaminopurine (BAP) on the synthesis of essential oils and the isomerization of methyl chavicol and trans-anethole in Ocimum gratissimum L.

    Science.gov (United States)

    Hazzoumi, Zakaria; Moustakime, Youssef; Amrani Joutei, Khalid

    2014-01-01

    Basil (O. gratissimum L) is a aromatic and medicinal plant widely used in traditional medicine in Morocco. The aim of this work was to study the effect of three plant growth regulators gibberellic acid (GA), indole 3-acetic acid (IAA) and benzylaminopurine (BAP) on the content and composition of essential oils of this plant, especially on the main compound (methyl chavicol) and its isomer (the trans-anethole). The results showed a wide variation on yield, content and range of the molecule constituent of oil, with a balance of appearances and/or disappearances of a few molecules. GA caused a slight decrease in the oil yield (0.2%), but it increased the diversity of compounds (17 molecules) with the appearance of four new compounds (naphthalene, camphor, germacrene-D, and ledene) and disappearance of (β cedrene, azulene). This variation also caused a very important decrease in the main compound (methyl chavicol) and increases its isomer (trans-anethole). IAA and BAP caused an increase in the yield of essential oil (0.30% and 0.32% respectively) without much influence on the main compounds, but with some change in the composition such as the appearance of (germacrene-D) and the disappearance of (aristolene).

  14. Efecto del carbón activado, ácido indolacético (AIA y bencil amino purina (BAP en el desarrollo de protocormos de Epidendrum elongatum Jacq bajo condiciones in vitro

    Directory of Open Access Journals (Sweden)

    Jaime Alonso Pedroza-Manrique

    2009-06-01

    Full Text Available The effect of activated charcoal, indol acetic acid (IAA and benzylaminopurine (BAP on Epidendrum elongatum Jacq protocorm-like body (PLB development in vitro conditions Resumen:  El desarrollo vegetativo de los protocormos de Epidendrum elongatum Jacq., una orquídea endémica y en vías de extinción, se logró eficazmente mediante el cultivo in vitro en el medio Murashige y Skoog (1962, donde se evaluó el efecto de la interacción entre los siguientes tres factores: carbón activado (0,0; 0,5; 1,0% (w/v; ácido indol acético (0,0; 0,5; 1,0 mg.L-1; bencil amino purina (0,0; 0,5; 1,0 mg.L-1. El medio de cultivo empleado fue enriquecido con sacarosa al 3%, y el Myo inositol al 0,1 g.L-1. En este estudio se evaluó que el efecto sobre la tasa de crecimiento de la interacción del carbón activado en concentraciones de 0,5 y 1,0% con 0,5 mg.L-1 de AIA es positivo para el desarrollo de los protocormos de E. elongatum bajo condiciones in vitro, mientras que la interacción del BAP, tanto en concentraciones de 0,5 mg.L-1 y 1,0 mg.L-1, muestra resultados no tan favorables en cuanto al grado de desarrollo de los protocormos estudiados. Este estudio describe un protocolo que permite alcanzar más de 270.000 plántulas en excelente desarrollo vegetativo dentro de 30 semanas, a partir de una cápsula de esta importante orquídea. Este protocolo es un modelo de conservación para especies que se encuentran en vías de extinción, y además permite la propagación a gran escala de Epidendrum elongatum. Palabras clave: orquídea, Epidendrum elongatum, germinación in vitro, carbón activado, AIA, BAP. Abstract: The vegetative development of protocorm-like bodies (PLB from Epidendrum elongatum Jacq. (an endemic and endangered orchid was effectively achieved through in vitro culture in Murashige and Skoog medium (1962; the effect of the interaction between the following three factors was studied: activated charcoal (0.0, 0.5, 1.0% (w/v, indol acetic acid

  15. Mutagenicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and benzo[a]pyrene (BaP) in the gill and hepatopancreas of rpsL transgenic zebrafish.

    Science.gov (United States)

    Amanuma, Kimiko; Tone, Suguru; Nagaya, Masato; Matsumoto, Michi; Watanabe, Tetsushi; Totsuka, Yukari; Wakabayashi, Keiji; Aoki, Yasunobu

    2008-10-30

    We examined the in vivo mutagenicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and benzo[a]pyrene (BaP) by using transgenic (Tg) zebrafish carrying the mutational target gene rpsL. PBTA-6 is one of the PBTA-type compounds that were recently identified in highly mutagenic river water in Japan. BaP is a well-known contaminant that is frequently found in polluted water. Both compounds are potent mutagens, as determined by using the Ames test employing S9 mix and Salmonella. Adult rpsL Tg zebrafish were exposed to 0, 7, or 10 mg/L PBTA-6 or 0, 1.5, or 3 mg/L BaP for 96 h in a water bath and the mutations in their gills and hepatopancreata were measured 2-4 weeks later. At 3 weeks after exposure, 3 mg/L BaP significantly increased the rpsL mutant frequency (MF) in the gill and hepatopancreas by 5- and 2.3-fold, respectively, as compared to control fish. Sequence analysis showed that BaP mainly induced G:C to T:A and G:C to C:G transversions, which is consistent with the known mutagenic effects of BaP. In contrast, despite its extremely high mutagenic potency in Salmonella strains, PBTA-6 did not significantly increase the MF in the zebrafish gill or hepatopancreas. Although PBTA-6 is 300 times more mutagenic than BaP in the Ames test [T. Watanabe, H. Nukaya, Y. Terao, Y. Takahashi, A. Tada, T. Takamura, H. Sawanishi, T. Ohe, T. Hirayama, T. Sugimura, K. Wakabayashi, Synthesis of 2-phenylbenzotriazole-type mutagens, PBTA-5 and PBTA-6, and their detection in river water from Japan, Mutat. Res. 498 (2001) 107-115], calculation of the mutagenicity per mole of compound indicated that PBTA-6 was 33- and BaP.

  16. Characterization of coagulase-negative staphylococcus species from cows' milk and environment based on bap, icaA, and mecA genes and phenotypic susceptibility to antimicrobials and teat dips.

    Science.gov (United States)

    Piessens, V; De Vliegher, S; Verbist, B; Braem, G; Van Nuffel, A; De Vuyst, L; Heyndrickx, M; Van Coillie, E

    2012-12-01

    The aim of this study was to investigate whether the main coagulase-negative staphylococci (CNS) species involved in bovine intramammary infections (IMI) possess specific characteristics that promote colonization of the udder. Virulence markers associated with biofilm formation, antimicrobial resistance, and biocide tolerance were compared between typically contagious CNS species (Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus simulans) and those rarely causing IMI (Staphylococcus sciuri, Staphylococcus equorum, and others) to find possible associations with pathogenicity. Coagulase-negative staphylococci isolates (n=366) belonging to 22 different species were analyzed by PCR for the presence of the biofilm-associated genes bap and icaA, and the methicillin resistance gene mecA. A selection of 82 isolates was additionally tested for their susceptibility to 5 antibiotics and 2 commercial teat dip products. Minimum inhibitory concentrations of antimicrobials were determined by Etest (AB bioMérieux, Marcy l'Etoile, France), and a microdilution method was optimized to determine minimum biocidal concentrations of teat dips. The bap, icaA, and mecA genes were detected significantly more in isolates from CNS species typically living in the cows' environment than in isolates from IMI-causing species. Antimicrobial resistance was mainly against erythromycin (23%) or oxacillin (16%), and was detected more often in the environmental species. The isolates least susceptible to the teat dips belonged to the IMI-causing species Staph. chromogenes and Staph. simulans. We concluded that carriage of biofilm genes and antimicrobial resistance were not associated with the ability to colonize the mammary gland because free-living CNS species constituted a more significant reservoir of biofilm and resistance determinants than did IMI-causing species. In contrast, increased tolerance to biocides may favor the establishment of

  17. Using an international workshop to develop functional requirements and a Biohazard Assessment Protocol (BAP) with which to develop a preferred concept for a Mars Sample Return (MSR) Sample Receiving Facility (SRF).

    Science.gov (United States)

    Vrublevskis, John; Guest, Michael; Bennett, Allan; Walker, James; Smith, Caroline; Baker, Robert; Grant, Colin

    COSPAR Planetary Protection Policy places very stringent requirements on Sample Return missions and `breaking the chain of contact' between Earth and the celestial body from which the sample is returned. This must be continued after return to Earth and -at the same time-any contamination or damage to the sample must be avoided until such time as it can be confirmed as not presenting a biohazard and available for subsequent curation and scientific investigation. Such a facility has never before been built and an investigation has been made to determine the requirements for a Mars Sample Return (MSR) Sample Receiving Facility (SRF) from first principles based on a Biohazard Assessment Protocol (BAP) rather than the modification of an existing BSL-4 facility. This approach fully takes in to account cleanliness requirements to avoid sample contamination. This paper will present the results from the first part of an ESA funded study, which derived the top level functional requirements by reviewing published scientific literature from all expert domains i.e.; biocontainment, science curation, space technology, infrastructure and equipment. These functional requirements were then examined by invited attendees from all expert domains in a 2 day international workshop. From the results of the international workshop an existing Biohazard Assessment Protocol (BAP) was modified and a trade off was conducted to derive a preferred concept which allowed early indications of future technological direction of study. The results of the MSR SRF study by SEA (as well as the results of another parallel study) will be taken into account by ESA to define the requirements for a follow-on procurement action in the future. There will be a review of the results from both studies and an agreement on the requirements based on these parallel studies. Therefore, the views expressed herein should not be taken to reflect the official opinion of the European Space Agency.

  18. Benzo [a]pyrene (BaP) promotes abdominal aortic aneurysm formation in mice and its mechanism%苯并[a]芘诱导小鼠腹主动脉瘤形成机制研究

    Institute of Scientific and Technical Information of China (English)

    蒋凤春; 季亢挺; 隋向前; 唐疾飞

    2014-01-01

    Objective To investigate the effect of benzo [a]pyrene (BaP) on the formation of abdominal aortic aneurysm (AAA) in mice and its mechanism. Methods C57BL/6N mice aged 7~9 months were divided into four groups, control group, angiotensinⅡ(AngⅡ) group, BaP group and AngⅡ/BaP group. After 5 weeks of treatment of various agents the abdominal aor-tas of mice were taken. Aortic tissues were subject to HE, immunochemistry staining for evaluation of vascular wal structure, the expression of matrix metal oproteinase- 3, nuclear factor- κB and the apoptosis of vascular smooth muscle cel s. Results AngⅡinfusion with BaP injection induced AAA in 41.67%of mice (versus 25%in AngⅡgroup, P<0.05). Histological analysis of the aortic wal showed severe damage to the medial layer and breakage of elastic lamel a. Immunohistochemistry staining showed that the expression of MMP- 3 and NF- κB were increased in AngⅡ/BaP group compared with AngⅡand BaP group (0.48± 0.13%vs 0.24±0.16%and 0.34±0.10%, P<0.05; 0.42±0.12%vs 0.23±0.06%and 0.32±0.09%, P<0.05). The apoptosis of aortic vascular smooth muscle cel s was highest in AngⅡ/BaP group compared with AngⅡand BaP group (30±12%vs 19±5%and 23±4%, P<0.05). Conclusion BaP promotes AngⅡ- induced AAA formation in aging C57BL/6N mice, which may be as-sociated with activating NF- κB, up- regulation of MMP- 3 expression, resulting in disarray of elastic lamella, apoptosis of vascu-lar smooth muscle cel s, vascular structural destroy and ultimately aneurysm formation.%目的:探讨香烟烟雾重要成分苯并[a]芘(BaP)诱导小鼠腹主动脉瘤(AAA)形成的相关机制。方法48只7~9月龄C57BL/6N小鼠分为对照组、血管紧张素Ⅱ(AngⅡ)组、BaP组、AngⅡ/BaP复合物组。5周后取各组小鼠腹主动脉观察大体标本形态,切片行HE染色观察血管结构;免疫组化法检测各组靶血管基质金属蛋白酶-3(MMP-3)、核转录因子-κappaB(NF-κB)的表达, POD

  19. Stable ion study of benzo[a]pyrene (BaP) derivatives: 7,8-dihydro-BaP, 9,10-dihydro-BaP and its 6-halo derivatives, 1- and 3-methoxy-9,10-dihydro-BaP-7(8H)-one, as well as the proximate carcinogen BaP 7,8-dihydrodiol and its dibenzoate, combined with a comparative DNA binding study of regioisomeric (1-, 4-, 2-) pyrenylcarbinols.

    Science.gov (United States)

    Okazaki, Takao; Laali, Kenneth K; Zajc, Barbara; Lakshman, Mahesh K; Kumar, Subodh; Baird, William M; Dashwood, Wan-Mohaiza

    2003-05-01

    A stable ion study of a series of BaP derivatives is reported. 7,8-Dihydro-BaP 1 gives a persistent bay-region benzyliclike carbocation which shows extensive charge delocalization into the pyrene moiety. In contrast, a "benzylic" carbocation can not be generated from 9,10-dihydro-BaP 2. Introduction of bulky substituents at peri C-6 of 9,10-dihydro-BaP (as in 4 and 5) prevents side reactions (dimerization) to the extent that the initially formed carbocation undergoes rearrangement to generate the corresponding bay-region "benzylic" carbocation as a persistent species. Introduction of methoxy substituents into the 1- or 3-positions of 9,10-dihydro-BaP-7(8H)-one (6,7) increases its electrophilic reactivity to the extent that stable carboxonium-arenium dications are produced in FSO3H-SO2ClF. A detailed NMR study (at 500 MHz) of the resulting mono- and dications is reported, and charge delocalization mode (as well as conformational aspects) are addressed. Other oxidized derivatives of BaP such as the 7,8-dihydrodiol 9 and the 7,8-dihydrodibenzoate 8 are not suitable models for stable ion study because of competing O-protonation (and elimination). Energies for various possible arenium ions and regioisomeric "benzylic" cations were computed by the DFT method at the B3LYP/6-31G(d)//B3LYP/6-31G(d) level or by AM1 for comparison with the experimental results. These findings provide further evidence in support of the stability sequence: 1-pyrenyl > 4-pyrenyl > 2-pyrenyl in alpha-pyrene-substituted carbocations as models for the intermediates arising from BaP-epoxide ring opening. In an effort to provide a parallel, a series of alpha-pyrenylcarbinols were subjected to a DNA binding study using human MCF-7 cells. The results/trends are discussed and compared with the stable ion data.

  20. Increase the foliage area of Asparagus Officinalis L. Cv. UC 157 F1 “asparagus” by the spraying of Gibberellin (AG3 and 6 – Benzilaminopurine (6 – BAP

    Directory of Open Access Journals (Sweden)

    José Paraguay Mercado

    2010-12-01

    Full Text Available The present work had as objective to study the increment of the leaf area of Asparagus Officinalis L., for they were used it asparagus crown with 5 yolks, to those that were applied by aspersion, different concentrations of the hormones of gibberellic acid and 6-bencilaminopurina which were applied at the 25, 60, 110 and 160 days respectively. It was found that in the different rehearsed treatments one doesn't observe differential significant in the increment of the number of plants, plant height, number of yolks and dry weight of foliage. However it was observed that the combined application of the phytohormonas in the range of 0.110 at 0.230 m of gibberllic acid and 0.037 to 0.075 m of 6-Bencilaminopurin a bigger number of yolk is achieved (13%, sprout (7%, plant height (11.3% and dry weight (7.3% in the treated plants. Concluded to continue making studies of sinergism of these two phytohormonas, gibberellic (AG3 and 6-bencilaminopurina (6-BAP, seeking to achieve adequate levels of leaf area increase of the leaf area in the asparagus.

  1. Expression of bacterial alkaline phosphatase-steroid receptor coactivator-1 fusion protein and its application in regulation of drug metabolism enzymes%BAP-SRC-1融合蛋白的表达及其在药物代谢酶调控研究中的应用

    Institute of Scientific and Technical Information of China (English)

    陈亚坤; 陈枢青; 曾苏

    2005-01-01

    目的获得活性表达的细菌碱性磷酸酶(bacterial alkaline phosphatase,BAP)基因和人甾体受体辅活化因子-1(stero1d receptor coactivator-1,SRC-1)的融合蛋白,应用于辅活化因子与核受体的结合研究.方法从Escherichia coli JM83基因组和人肝总RNA中分别扩增获得BAP基因和SRC-1的186个氨基酸对应的基因序列(简称SRC186).用重组技术,构建BAP-SRC186-pET28a融合基因表达载体,在Escherichia coli Rosetta(DE3)中,以IPTG低温诱导表达.以对硝基苯磷酸盐(p-nitrophenyl-phos-phate,PNPP)为底物进行活性测定.活性表达的BAP-SRC186融合蛋白被应用于辅活化因子与核受体的结合研究.结果获得可溶性融合蛋白BAP-SRC186.该融合蛋白的BAP比活为(0.176±0.013 4)μmol·min-1·mg(pro).在利福平存在的情况下,BAP-SRC186能与孕烷X受体配体结合域(pregnane X receptor ligand binding domain,PXRLBD)发生利福平剂量依赖性的相互作用,作用强度通过BAP的显色反应可方便地检测.结论 BAP融合蛋白与核受体的结合研究为药物代谢酶调控的体外研究开辟了新的思路.

  2. Distribution of Bioavailable Phosphorus (BAP) in lake sediments of the middle and lower reaches of the Yangtze River%长江中下游湖泊沉积物生物可利用磷分布特征

    Institute of Scientific and Technical Information of China (English)

    张路; 范成新; 朱广伟; 王建军

    2006-01-01

    利用化学提取方法对太湖6个样点,巢湖4个样点和龙感湖3个样点的表层沉积物和沉积物柱样进行了生物可利用磷(BAP)测定.北太湖表层沉积物的平均含量为259.5mg/kg,而西部湖区平均含量为114.6mg/kg,湖心区平均含量为40.6 mg/kg,而东太湖平均含量为50.7 mg/kg,呈显著北高南低的特点.巢湖西部湖区表层沉积物的BAP平均含量为254.2mg/kg,而东部湖区BAP含量降低为101.9mg/kg.龙感湖表层沉积物BAP平均含量为67.8mg/kg.显著表明污染程度较高的湖区沉积物的BAP相应较高.BAP在沉积物中随深度呈指数降低,显示生物可利用磷在沉积作用下向稳定的非活性磷转化.夏季沉积物中的BAP由于生物活性的增强向溶解态活性磷转化过程增强,显示为较低的BAP含量.BAP含量与水体溶解态活性磷呈正相关关系,且该相关性在BAP含量较低的样点好于高BAP的样点.

  3. 五味子乙素对BaP致HTR8-SVneo细胞损伤的保护作用及机制%The Protection Effect and Mechanism of Schisandrin B on Extravillous Trophoblast Cell Line Damage Caused by BaP

    Institute of Scientific and Technical Information of China (English)

    吴念; 陈亚琼; 陈晓; 侯海燕; 王华

    2016-01-01

    目的:研究五味子乙素(Schisandrin B,Sch B)预防苯并芘(BaP)致人绒毛膜外滋养层细胞(HTR8-SVneo)损伤的作用机制.方法:通过新型四唑化合物(MTS)法观察和测定,最终选取0.5,1,2μmol/L Sch B分别作用HTR8-SVneo细胞24 h后再给予20μmol/L Bap染毒处理24 h,MTS检测各组细胞生长情况;JC-1荧光染色法观察各组线粒体膜电位荧光强度变化;酶联免疫吸附试验(ELISA)检测各组细胞内促凋亡蛋白(Bax)、细胞色素C (cytochromec,cyt-c)蛋白浓度;蛋白质印迹法(Western blotting)检测各组的凋亡蛋白(Caspase-9).结果:①BaP染毒组OD值为1.187±0.015,0.5,1,2μmol/L Sch B预保护24 h后的OD值分别为1.483±0.022、1.489±0.048和1.531±0.240,差异有统计学意义(P<0.05).②BaP染毒组线粒体荧光强度弱,0.5,1,2μmol/L Sch B预保护24 h后的荧光强度较强,差异有统计学意义(P<0.05).③BaP染毒组Bax、cyt-c蛋白浓度升高,0.5,1,2μmol/L Sch B预保护24 h后细胞内Bax、cyt-c蛋白浓度降低,差异有统计学意义(P<0.05).④BaP染毒组细胞内caspase-9蛋白表达升高,0.5,1,2μmol/L Sch B预保护24 h后细胞内caspase-9蛋白表达降低,差异有统计学意义(P<0.05).结论:Sch B可以预防BaP致HTR8-SVneo细胞损伤,其作用机制可能与抗线粒体途径有关.%Objective:To explore the protection mechanism of Schisandrin B on extravillous trophoblast cell line damage caused by BaP. Methods:After 0.5,1,2μmol/L Sch B deal with human HTR8-SVneo cells 24 h,added 20μmol/L Bap 24 h, using MTS measured cells growth. Used JC-1 fluorescent staining to measure the change of mitochondrial membrane potential in each group. Respectively used ELISA to measure the concentration of Bax and cyt-c protein in each group. Respectively used Western blotting to analyse the expression of caspase-9 in each group. Results: ①MTS results showed that BaP alone cell OD was 1.187±0.015,0.5,1,2μmol/L Sch B the role of OD was 1.483±0.022,1.489±0.048 and 1

  4. Bap: A New Type of Functional Amyloid.

    Science.gov (United States)

    Di Martino, Patrick

    2016-09-01

    Bacteria can build a biofilm matrix scaffold from exopolysaccharides or proteins, and DNA. In a recent report, Taglialegna and colleagues show that pathogenic Staphylococcus aureus produces a protein scaffold based on amyloid assembly of fragments from the biofilm-associated protein. Amyloidogenesis occurs in response to environmental signals.

  5. Bap: A New Type of Functional Amyloid.

    Science.gov (United States)

    Di Martino, Patrick

    2016-09-01

    Bacteria can build a biofilm matrix scaffold from exopolysaccharides or proteins, and DNA. In a recent report, Taglialegna and colleagues show that pathogenic Staphylococcus aureus produces a protein scaffold based on amyloid assembly of fragments from the biofilm-associated protein. Amyloidogenesis occurs in response to environmental signals. PMID:27451288

  6. Study on serum BAP and OC of tibetan adolescents%藏族青少年骨碱性磷酸酶与骨钙素水平分析

    Institute of Scientific and Technical Information of China (English)

    王平; 王红梅; 温友峰; 许德顺; 席焕久

    2007-01-01

    目的 探讨西藏拉萨与那曲地区藏族青少年血清骨碱性磷酸酶(BAP)和骨钙素(OC)的特点及其差异.方法 选取拉萨和那曲地区12~18岁健康藏族青少年共1 458人,测定身高及血清BAP的活性及OC的含量.结果 拉萨男性血清BAP、OC在13~14岁均出现高峰;女性12岁时值最高.而那曲地区男女性血清BAP、OC水平在大部分年龄组明显低于拉萨男女性(P<0.05).男性BAP、OC出现高峰的年龄提前1年.2组随年龄增大,血清BAP、OC值均明显下降(P<0.05).但拉萨女性在16岁以后,那曲男性16岁以后、女性15岁以后,各年龄组血清BAP、OC水平差异无统计学意义(P>0.05).血清BAP、OC水平均与身高密切相关(r=0.42,0.41,P<0.01).结论 拉萨与那曲藏族青少年男女血清BAP和OC水平均与年龄、性别呈显著相关,但那曲地区青少年BAP和OC水平均低于拉萨地区青少年,且高峰期提前,高水平持续时间短,可能是该地区身高较矮的原因之一.

  7. Significance of BAP and PSA in diagnosis of prostate cancer bone metastases%血清BAP和PSA测定在前列腺癌骨转移诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    季鸿; 于建芳; 袁志斌; 朱瑞森

    2005-01-01

    我们分析、比较了骨源性碱性磷酸酶(BAP)和前列腺特异抗原(PSA)对前列腺癌骨转移的诊断意义,并探讨了二者在前列腺癌骨转移分级中的应用价值.对68例前列腺癌病人进行骨显像,根据骨显像结果及临床表现分为骨转移组与非骨转移组.骨显像依Soloway分级标准分为五级(M0-M4).对所有病人进行血清BAP与PSA检测,各组之间BAP值及PSA值比较采用t检验.对转移级别的升高与BAP值之间进行相关分析.结果显示骨转移的级别升高与BAP呈正相关.(r=1.1083,P<0.01). PSA仅在M0与M1-M3之间有显著差异.BAP对前列腺癌骨转移最佳检测范围为25-35 u/L,阳性预测率为96.6%,阴性预测率为75%.BAP与PSA值分别为35 u/L和20ng/mL时,骨转移阳性预测率为97.5%.BAP与PSA值分别为25 u/L和20ng/mL时,阴性预测率达73.9%.由此我们得出:1.BAP预测前列腺癌骨转移比PSA有效,并且BAP对前列腺癌骨转移的分级有诊断价值;2.BAP与PSA联合应用可提高前列腺癌病人骨转移的阳性预测率.

  8. 唑来膦酸、降钙素对骨生化标志物BAP、N-MID、β-CTX的影响%Effect of zoledronic acid and calcitonin on bone metabolic biochemical markers, BAP, N-MID, and β-CTX

    Institute of Scientific and Technical Information of China (English)

    孔西建; 吴丹; 叶进; 廉杰

    2013-01-01

    目的 通过监测绝经后骨质疏松症患者使用唑来膦酸、降钙素治疗前后骨代谢生化标志物的变化来分析二者对骨代谢的影响.方法 回顾性分析绝经后骨质疏松症病例115例,55例使用唑来膦酸(5mg静滴一次),60例使用鲑鱼降钙素(50IU隔日一次肌注,治疗90日),检测每个病例治疗前和开始治疗后4周、8周、12周的BAP、N-MID、β-CTX,并进行统计学分析.结果 降钙素组治疗4周后BAP、β-CTX与治疗前相比降低(P<0.05),骨转换率降低;治疗8周、12周后β-CTX仍明显降低(P<0.05),但BAP与治疗前相比变化无统计学意义;唑来膦酸组治疗4周、8周后BAP、N-MID、β-CTX与治疗前相比均明显降低(P<0.05),骨转换率明显降低;治疗后12周后BAP、β-CTX仍明显降低(P<0.05);与降钙素组相比,唑来膦酸组治疗后4周、8周BAP、N-MID、β-CTX降低更明显(P<0.05),骨转换率降低更明显;治疗后12周BAP、β-CTX仍明显降低(P<0.05).两种药物均具有较好的安全性.结论 降钙素组、唑来膦酸组均可有效抑制骨吸收,降低骨转换率;与降钙素相比,唑来膦酸抑制骨吸收的作用更迅速、更稳定,病人低骨转换持续时间更久.%Objective To analyze the effect of zoledronic acid or calcitonin on bone metabolism by monitoring the changes of bone metabolic markers before and after the treatment of zoledronic acid or calcitonin in patients with postmenopausal osteoporosis. Methods The data of 115 patients with postmenopausal osteoporosis was analyzed retrospectively. Fifty-five patients were treated with zoledronic acid ( 5mg intravenous drip once ). Sixty patients were treated with salmon calcitonin ( 50U intramuscular injection every other day, lasting for 90 days ). BAP, N-MID , and β-CTX was tested before and 4-, 8-, and 12-week after the treatment. All the results were analyzed statistically. Result BAP and β-CTX in patients in calcitonin treatment group after 4-week treatment were

  9. Effect of Kaempferol on the BaP Induced Apoptosis in Human Chorionic Trophoblast Cells HTR8/SV neo%山奈酚对BaP引起的人绒毛膜外滋养层细胞HTR8/SV neo凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    连立芬; 陈亚琼; 侯海燕; 曹波; 吴念

    2014-01-01

    目的:研究山奈酚对苯并芘(BaP)引起的人绒毛膜外滋养层细胞HTR8/SV neo凋亡的影响。方法:通过四甲基偶氮唑盐(MTT)法观察及测定,最终选取10μmol/L BaP和0.01,0.1,1μmol/L山奈酚共同处理人HTR8/SV neo细胞48 h,光镜下观察不同浓度BaP作用下细胞生长情况,吉姆萨(Gimesa)染色及Hochest33342荧光染色观察细胞凋亡情况,利用流式细胞术测定细胞凋亡率变化。结果:①流式细胞术测定结果表明BaP单独作用细胞存活率为(72.58±0.29)%,与0.01,0.1,1μmol/L山奈酚同时作用时细胞存活率分别为(84.96±1.34)%、(89.54±1.64)%和(91.01±1.26)%,两者同时作用组细胞存活率均高于BaP单独作用组,差异均有统计学意义(P<0.01)。②单独BaP作用可引起细胞染色质浓缩,出现凋亡小体等典型凋亡形态、细胞核致密浓染、碎裂状。结论:山奈酚对BaP引起的HTR8/SV neo细胞凋亡有明显的抑制作用。%Objective:To study the effect of kaempferol on the BaP induced apoptosis in human chorionic trophoblast cells HTR8/SV neo. Methods:10μmol/L BaP were added 0.01,0.1,1μmol/L kaempferol jointly deal with human HTR8/SV neo cells 48 h,observed cells growth under the inverted microscope,Gimesa staining and Hochest33342 fluorescent staining observed morphologic changes,determination of apoptosis rate changes by flow cytometry. Results: ①Flow cytometry results showed that BaP alone cell viability was (72.58 ±0.29)%, with 0.01,0.1,1 μmol/L kaempferol the role of cell viability was (84.96 ±1.34)%, (89.54 ±1.64)% and (91.01 ±1.26)%, respectively, compared with BaP alone group, the differences were statistically significant (P<0.01). ②BaP alone can cause cell chromatin condensation,apoptotic bodies and other typical apoptotic morphology,dense hyperchromatic nuclei,fragmentation shape. Conclusions:Kaempferol showed a protective effect on BaP induced apoptosis in

  10. Multiplicación de Selenicereus megalanthus (pitahaya amarilla e Hylocereus polyrhizus (pitahaya roja vía organogénesis somática

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    Rocío Stella Suárez Román

    2014-09-01

    Full Text Available Pitahaya amarilla (Selenicereus megalanthus y roja (Hylocereus polyrhizus se multiplicaron vía organogénesis somática, a partir de hojas cotiledonares y fragmentos de cladodio provenientes de plántulas con 20 días de desarrollo, en medio Murashige & Skoog (MS suplementado con auxinas (ANA y citocininas (Kinetina y BAP. En el medio de cultivo M3 (BAP + Kinetina 2 mg/l se obtuvieron entre cuatro y nueve brotes enraizados de 4 cm de longitud, en un periodo de 30 días. En pitahaya roja se desarrolló mayor número de brotes. En todos los medios estudiados se observó formación de callo, siendo más alto el número en el medio con altas concentraciones de ANA. Las vitroplantas en fase de climatización alcanzaron 100% de sobrevivencia y presentaron respuestas diferenciales al sustrato empleado.

  11. TCDD和B[a]P诱导大鼠肺癌变过程CYPIA1表达变化的研究%Expression of CYPIA1 in the process of rat lung carcinogenesis induced by TCDD and B[a]P

    Institute of Scientific and Technical Information of China (English)

    肖和龙; 吕嘉春; 黎银燕; 宾晓农; 谭敏; 刘小琦

    2013-01-01

    目的:利用四氧二苯二氧杂环己二烯(TCDD,二噁英)和苯并(a)芘(B[a]P)致大鼠肺癌的动物模型,研究肺癌变过程中CYPIA1基因mRNA和蛋白水平的表达.方法:应用RT-PCR方法及荧光免疫法检测大鼠正常肺组织、癌前病变组织和肺癌组织中CYPIA1基因mRNA的表达及CYPIA1基因在肺组织蛋白水平的表达,评价CYPIA1基因在肺癌变过程的作用.结果:CYPIA1 mRNA/β-actin比值在TCDD、TCDD+ B[a]P、B[a]P致癌组及对照组分别为0.956 4±0.328 2、1.153 3±0.432 6、0.252 4±0.103 2和0.093 6±0.032 7;在大鼠正常肺组织、正常增生、癌前病变和肺癌组织中分别为0.105±0.098、0.235±0.108、1.127±0.295和1.564±0.421.结果表明,CYPIA1 mRNA在TCDD+B[a]P、TCDD致癌组有较强表达,在肺癌前病变和肺癌组织中有较强表达;CYP1A1蛋白表达灰度值在TCDD、TCDD+ B[a]P、B[a]P致癌组及对照组分别为41.76±4.82、46.28±4.32、31.02±3.16和18.75±2.27,在大鼠肺正常组织、正常增生、癌前病变和肺癌组织中分别为19.77±2.74、26.71±3.32、43.95±3.98和47.38±4.96.结果表明,CYP1A1蛋白在TCDD+ B[a]P和TCDD致癌组有较强表达,在肺癌前病变和肺癌组织中有较强表达.结论:TCDD和B[a]P对CYPIA1有较好的诱导作用,CYPIA1高表达与肺癌的发生有着密切的关系,其在肺癌前病变和肺癌组织中的高表达,可能是肺癌前病变、肺癌发生的一个标志.%OBJECTIVE:To investigate the role of gene CYPIA1 in the process of lung carcinogenesis. METHODS: In the animal model of rats lung cancer induced by dioxin and B[a]P,the level of CYPIA1 mRNA and CYPIA1 protein expression in groups of TCDD, TCDD + B[a]P, B[a]P and control group were tested. RESULTS: The ratio of CYPIAlmRNA/p-actin in cancerogen groups of TCDD,TCDD+B[a]P, B[a]P and control group were 0. 956 4±0. 328 2, 1. 153 3 + 0. 432 6,0. 252 4 + 0. 103 2,0. 093 6 + 0. 032 7;in normal epithelium,epithelial hyperplasia,atypical hyperplasia

  12. Organogênese in vitro a partir de diferentes regiões do epicótilo de Citrus sp Citrus sp. organogenesis in vitro from different epicotyl's regions

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    Evandro Henrique Schinor

    2006-12-01

    Full Text Available O estabelecimento de protocolos para regeneração de plantas in vitro é essencial para o uso de técnicas de transformação genética no melhoramento de citros. Visando à obtenção de um protocolo eficiente de regeneração in vitro para laranja-azeda (Citrus aurantium, laranjas 'Natal' e 'Pêra' (C. sinensis, limão 'Volkameriano' (C. volkameriana e citrange 'Carrizo' (Poncirus trifoliata x C. sinensis, avaliou-se a resposta morfogênica de diferentes regiões do epicótilo (basal, mediana e apical em relação a distância do nó cotiledonar, na presença (1,0 mg/L-1 ou ausência de 6-BAP, em meio de cultura MT. Após 60 dias, avaliaram-se a porcentagem de explantes responsivos e o número de gemas adventícias por explante. A resposta morfogênica em função da região do epicótilo e da presença ou ausência da citocinina (6-BAP foi influenciada pelo genótipo. A presença de 6-BAP no meio de cultura promoveu aumento na porcentagem de explantes responsivos para citrange 'Carrizo'. A suplementação do meio de cultura com a citocinina 6-BAP proporcionou aumento no número de brotos por explante para citrange 'Carrizo', laranja 'Natal' e limão 'Volkameriano'.The establishment of an in vitro plant regeneration protocol is essential for the use of genetic transformation techniques in Citrus breeding programs. Aiming to obtain an efficient protocol of in vitro regeneration for sour orange (Citrus aurantium, sweet oranges 'Natal' and 'Pêra' (C. sinensis, 'Volkamer' lemon (C. volkameriana and 'Carrizo' citrange (Poncirus trifoliata x C. sinensis, the morphogenetic response was evaluated for different epicotyl's regions (basal, medium and apical regarding the distance from the cotyledonary node, in presence (1,0 mg/L-1 or absence of 6-BAP in MT medium. After 60 days, the percentage of responsive explants and number of shoots per explants were evaluated. The morphogenetic response related to the epicotyl's region and the presence or absence

  13. Multiplicação in vitro de segmentos apicais caulinares de segurelha (Satureja hortensis L.

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    M.C.I Navroski

    2014-03-01

    Full Text Available O presente estudo teve por objetivo avaliar o efeito de BAP, na presença e ausência de ANA, sobre a multiplicação in vitro de segmentos apicais caulinares de Satureja hortensis. Os explantes foram isolados de plântulas germinadas in vitro e cultivados em meio nutritivo MS. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 2x5, correspondendo às concentrações de ANA (0 e 1 µM e de BAP (0; 5; 10; 15 e 20 µM, com seis repetições, cada uma composta por três explantes. Para a porcentagem de explantes com brotações houve efeito significativo para o fator BAP, aumentando à medida que cresce a concentração da citocinina. A variável "número de brotos por explante" apresentou interação entre os fatores, havendo a maior formação de brotos na presença de ANA na faixa de 10 - 15 µM de BAP. Na presença da auxina, o maior valor ocorreu com 15 µM de BAP tendendo a diminuir independente da presença de ANA. O enraizamento dos segmentos apicais foi maior na presença de ANA e ausência de BAP, diminuindo com o aumento da citocinina. O número de folhas foi influenciado pela maior concentração de BAP sendo que a 15 µM foi observado o maior número, tendendo à queda com aumento da concentração. A concentração de 15 µM de BAP, independente de ANA, proporcionou os melhores resultados na multiplicação da espécie produzindo aumento de brotações e número de folhas, à exceção do enraizamento que foi influenciado pela auxina.

  14. MICROPROPAGAÇÃO DO KIWI CV. HAYWARD

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    SCALIZE FÁBIO EDUARDO

    2001-01-01

    Full Text Available O presente trabalho teve como objetivo desenvolver um protocolo para a obtenção de mudas de kiwi (Actinidia chinensis Planch, cv. Hayward, por meio do cultivo in vitro de cotilédones. Utilizou-se o meio de MURASHIGE & SKOOG (1962 -- MS, suplementado com dois tipos de auxina (AIA e AIB e uma citocinina (BAP. Foram verificados os efeitos de três doses de auxinas (0,125; 0,250 e 0,375 mg.L-1, combinadas com três doses de citocinina (0,5; 1,0 e 1,5 mg.L-1 na capacidade morfogênica dos explantes. Procedeu-se o estudo histológico dos órgãos das plântulas obtidas in vitro, e verificou-se, também, a capacidade de aclimatação das mudas ex vitro. A menor dose de AIB (0,125 mg.L-1, independentemente das doses de BAP, foi a mais eficaz na morfogênese dos explantes. Não foram verificadas alterações histológicas e anatômicas das plântulas obtidas in vitro. Aos três meses após o cultivo ex vitro dos explantes, verificou-se a sobrevivência de 88% das plantas transplantadas em condições de campo.

  15. ESTABELECIMENTO IN VITRO DE PORTA-ENXERTOS DE VIDEIRA ATRAVÉS DE ÁPICES MERISTEMÁTICOS E SEGMENTOS NODAIS

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    BIASI L.A.

    1998-01-01

    Full Text Available Visando aumentar a eficiência de estabelecimento in vitro dos porta-enxertos de videira `Jales' e `Campinas', foram conduzidos alguns experimentos com ápices meristemáticos e segmentos nodais, retirados de brotações de estacas lenhosas. Foram testadas diversas concentrações de 6-benzilaminopurina (BAP em meio de cultura MS com a metade da concentração de sais. O efeito desta citocinina foi marcante sobre o crescimento dos explantes. O aumento da concentração de BAP estimulou o crescimento e expansão foliar dos ápices meristemáticos e das gemas axilares até a concentração de 10µM. O uso de 20µM de BAP reduziu o crescimento dos segmentos nodais. Conclui-se que o estabelecimento dos porta-enxertos pode ser realizado em meio MS, com a metade da concentração de sais, suplementado com 10µM de BAP.

  16. Multiplicação in vitro e alongamento das brotações micropropagadas do porta-enxerto 'Tsukuba 1'(Prunus persica L. In vitro multiplication and elongation of the micropropagation shoots of the roostock of 'Tsukuba 1' (Prunus persica L.

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    Elizete Beatriz Radmann

    2009-09-01

    Full Text Available Objetivou-se avaliar o efeito da fonte e concentração de citocinina e da composição dos sais presentes no meio de cultivo, na multiplicação in vitro do porta-enxerto 'Tsukuba 1' (Prunus persica L.. Explantes cultivados em meio QL/MS com 2,0 mg L-1 de BAP apresentaram maior formação de brotações e crescimento destas. Porém, é necessária a transferência dos explantes para o meio de cultura com redução na concentração de BAP (0,5 mg L-1 para as brotações adquirirem tamanho adequado para a fase de enraizamento.The objective was to assess the font and concentration's effect of cytokinin and salt composition, present in the medium of cultivation, on in vitro multiplication of the rootstock 'Tsukuba 1' (Prunus persica L.. Explants cultivated in QL/MS medium enriched with 2.0 mg/L of BAP showed more shoot formation and growth. However, it is necessary to transfer the explants to culture medium with reduced BAP concentration (0.5 mg/L, so that shoots may obtain the properly size for the rooting phase.

  17. Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia Multiplication in vitro of grapevine rootstock 'VR043-43' (Vitis vinifera x Vitis rotundifolia

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    Marília Pereira Machado

    2006-08-01

    Full Text Available A micropropagação é uma técnica que possibilita a propagação massal de plantas de interesse econômico. Objetivou-se com este trabalho avaliar o efeito de 6-benzilaminopurina (BAP e cinetina (CIN na multiplicação do porta-enxerto de videira 'VR043-43'. O isolamento foi realizado em meio de cultura QL, suplementado com BAP ou CIN, nas concentrações de 0; 2,5; 5,0 e 10,0µM. Os subcultivos foram realizados a cada 45 dias. O número de brotações por explante aumentou ao longo dos subcultivos com BAP. Esse efeito não foi observado com a CIN, que obteve resultado semelhante ao da testemunha (1,0 brotação/explante. As concentrações de 5,0 e 10,0 µM de BAP promoveram o maior número de brotações, porém com redução da altura. A concentração mais elevada de CIN (10,0 µM, também teve efeito negativo na altura das brotações, assim como no número de folhas por brotação. A formação de calo (100% foi observada com BAP e CIN em todas as concentrações, e na ausência de citocinina não houve a formação de calo. A citocinina BAP reduziu a formação de raízes, que foi de 100% com a CIN e a testemunha.The micropropagation is one technique that makes possible the massal propagation of plants of economic interest. The objective of this work was to evaluate the effect of 6-benzilaminopurina (BAP and kinetin (KIN in the multiplication in vitro of the grapevine rootstock 'VR043-43'. The isolation was carried through in culture medium QL, supplemented with BAP or KIN, in the concentrations of 0; 2,5; 5,0 and 10,0µM. The subcultures had been carried out each 45 days. The number of shoots per explant increased along with the subcultures with BAP. However this effect was not observed with the KIN that it got resulted similar to the one of the cytokin-free (1,0 shoot/explant. The concentrations of 5,0 nd 10,0 µM of BAP had promoted the biggest number of shoots with reduction of the height. The highest concentration of KIN (10,0 µ

  18. Propagação in vitro de Ocimum basilicum L. (Lamiaceae - DOI: 10.4025/actascibiolsci.v25i2.2034 In vitro propagation of Ocimum basilicum L. (Lamiaceae

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    Fabiana Kommling Seixas

    2003-04-01

    Full Text Available Ocimum basilicum L. (manjericão pertencente à família das Lamiaceae é rico em óleos essenciais aromáticos e valorizado por suas propriedades farmacêuticas, aromáticas e culinárias. Neste trabalho descrevemos o procedimento para micropropagação de O. basilicum utilizando cotilédones de sementes germinadas in vitro. Estas foram utilizadas como explante inicial, colocadas em meio MS (Murashige e /skoog, 1962, com 0,2mg.L-1 de ANA em combinação com BAP nas variações de 0–5mg.L-1 e mantidas à 28 ± 1°C, fotoperíodo de 16h de luz e densidade de fluxo luminoso de 48µmol.m-2.s-1 por 45 dias. Maior eficiência na formação de brotos foi obtida utilizando 5mg.L-1 BAP e 0,2mg.L-1 ANA. A presença de ANA inibiu a formação de raízes quando combinada com diferentes concentrações de citocinina (BAP, 1 a 5mg.L-1. Altas concentrações de BAP induziram aumento no número de explantes com brotos e no número de brotos/cotilédoneOcimum basilicum L. (sweet basil, of the family Lamiaceae, is rich in aromatic essential oils and valuable for its pharmaceutical, aromatic and culinary properties. The present study describes the procedure for micropropagation of O. basilicum using cotiledonary leafs from in vitro geminated plants. Cotyledons from in vitro germinated seeds were used as initial explants, put in MS (Murashige and Skoog, 1962 medium with 0.2mg.L-1 NAA (1-Naphthalene acetic acid in combination with 0–5mg.L-1 BAP(6-Benzyl aminopurine and kept at 28 ± 1ºC, 16-h light photoperiod and 48µmol.m-2.s-1 luminous density flow, for 45 days. The highest efficiency of shoot formation after 45 days occurred in the medium containing 5mg.L-1 BAP and 0,2mg.L-1 NAA. The presence of NAA inhibited root formation, when combined with different concentrations of cytokinin (BAP, 1 to 5mg.L-1. Higher BAP concentrations induced an increase in the number of explants with shoots and a higher number of shoots/cotyledon

  19. Multiplicação in vitro de gemas axilares de acácia-negra (Acacia mearnsii De Wild. In vitro multiplication of black watlle (Acacia mearnsii De Wild. axillary buds

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    Norton Borges Júnior

    2004-08-01

    Full Text Available A acácia-negra (Acacia mearnsii De Wild. é uma espécie de difícil micropropagação devido à pequena capacidade de multiplicação e desenvolvimento de gemas. O presente estudo visou determinar a influência de diferentes citocininas na proliferação de gemas axilares em segmentos nodais de A. mearnsii. Plântulas germinadas in vitro forneceram explantes que foram inoculadas em meio básico B5 (GAMBORG et al., 1968. Testaram-se as citocininas: BAP (6-benzilaminopurina, BA (6-benziladenosina, 2iP (gama,gama-isopenteniladenina e cinetina (6-furfuralamino-purina. Diferentes concentrações desses reguladores de crescimento foram empregadas: 1 mgL-1, 2 mgL-1 e 3 mgL-1. Utilizou-se o delineamento de blocos casualizados, em arranjo fatorial, com seis repetições e cinco plantas por parcela. As avaliações foram feitas aos 30 dias, através da contagem de gemas alongadas e da presença de calos. A utilização de BAP a 2 mgL-1 promoveu a maior taxa de multiplicação de gemas (3,5 brotos/explante.Black wattle (Acacia mearnsii de Wild. is difficult to micropropagate due to the low ability of multiplication and development of shoots. Thus, the present study aimed at determining the influence of various cytokinins on axillary bud proliferation in nodal segments of A. mearnsii. Explants from in vitro germinated seedlings were inoculated on B5 (Gamborg et al., 1968 basal medium. BAP (6-benzylaminopurine, BA (6-benzyladenine, 2iP (gamma,gamma-dimethylallylamino-purine and Kinetin (6-furfurylaminopurine were tested at the concentrations 1 mgL-1, 2 mgL-1, and 3 mgL-1. A randomized block design, in factorial arrangement with 6 replications, and 5 plants per plot was used. The assessments were made after 30 days, by counting the elongated shoots and the presence of callus. The use of BAP at 2 mgL-1 promoted the highest rate of bud multiplication (3,5 shoots/explant.

  20. Propagação in vitro de Sacha inchi In vitro propagation of Sacha inchi

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    Stevan Ricardo Bordignon

    2012-07-01

    Full Text Available O objetivo do presente trabalho foi avaliar in vitro a relação auxina:citocinina a fim de obter propágulos nos segmentos distintos do epicótilo e hipocótilo de sementes germinadas in vitro de Sacha inchi (Plukenetia volubilis Linneo. Os segmentos apical (A, mediano (B e o basal (C foram introduzidos em meio de cultivo MS, semi sólido (2,0g L-1 Phytagel, suplementado com vitaminas de MS, sacarose (30,0g L-1 e submetidos a três doses da auxina ácido indolbutírico - IBA (0; 0,1; 0,5mg L-1, associadas a quatro doses da citocinina benzilaminopurina - BAP (0; 0,1; 0,5; 1,0mg L-1, totalizando 36 tratamentos. Após nove semanas de cultivo in vitro, o segmento apical (A apresentou formação de brotações por organogênese direta nas concentrações de 0,5 e 1,0 de BAP, associado a 0,0 e 0,1 de IBA. O emprego do cultivo in vitro é viável na produção de mudas, utilizando como explante a região apical de sementes germinadas in vitro.The aim of this study was to perform an in vitro evaluation of the auxin:cytokinine ratio in different segments of the epicotyl and hypocotyl of Sacha inchi (Plukenetia volubilis Linneo seeds germinated in vitro. The segments apical (A, median (B and basal (C were introduced into semi-solid MS culture medium (2.0g L-1 Phytagel, supplemented with MS vitamins, sucrose (30.0g L-1 and submitted to three doses of auxin indolebutyric acid - IBA (0; 0.1; 0.5mg L-1, associated with four doses of the cytokinine benzylaminopurine - BAP (0; 0.1; 0.5; 1.0mg L-1, totaling 36 treatments. After nine weeks of in vitro cultivation, the apical segment (A presented shoot formation by direct organogenesis at the concentrations of 0.5 and 1.0 of BAP associated with 0.0 and 0.1 of IBA. It is feasible to use in vitro cultivation with the apical region of seeds germinated in vitro used as explants.

  1. Callus formation in vitro and internodal stem apices in savory = Calogênese in vitro de segmentos apicais caulinares e internodais em segurelha (Satureja hortensis L.

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    Marcio Carlos Navroski

    2012-12-01

    Full Text Available We sought to evaluate with this work different growth regulators on callus formation in shoot apical and internodal stem segments of Satureja hortensis. The explants were isolated from in vitro seedlings and cultured on MS nutrient medium supplemented with NAA (0 and 1 μM and BAP (0, 5, 10, 15 and 20 μM. The presence of auxin NAA gave higher marks to the calluses at 30 days of evaluation, these notes also increased with the addition of BAP. There were significant interactions between factors in evaluating NAA and BAP for 60 days, both in apical stem segments as in internodal stem segments. To stemapices percentage of friable callus tends to decrease with increasing concentration of BAP. As for compact calluses increased BAP leads to an increase in the percentage of this type of callus. The presence of NAA increased callus formation in both friable and compact calluses on. This trend was also observed in internodal stem segments. The callus formation was highly rhizogenic observed in the presence of NAA, hardly occurs in the absence of auxin. The use of BAP is recommended in case of regeneration of plants through micropropagation, if the goal is the production of metabolites, the use of BAP can be harmful by reducing the production of friable callus.= Buscou-se com este trabalho avaliar diferentes reguladores de crescimento sobre a calogênese em segmentos caulinares apicais e internodais de Satureja hortensis L.. Os explantes foram isolados de plântulas germinadas in vitro e cultivados em meio nutritivo MS acrescido de ANA (0 e 1 μM e de BAP (0; 5; 10; 15 e 20 μM. A presença da auxina ANA proporcionou notas mais altas para os calos aos 30 dias de avaliação, estas notas também aumentaram com o acréscimo da citocinina BAP. Houve interações significativas entre os fatores ANA e BAP na avaliação aos 60 dias, nos dois tipos de segmentos caulinares(apicais e internodais. Nos segmentos apicais caulinares a porcentagem de calos fri

  2. Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia Embriogênese somática e regeneração de plantas in vitro a partir de primórdios foliares de pupunheiras adultas

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    Marcílio de Almeida

    2006-09-01

    Full Text Available The aim of this work was to evaluate a protocol for plant regeneration by means of somatic embryos obtained from isolated adult pejibaye leaf primordia, and to describe histological origin of embryos and morphogenetic response. Explants were cultivated in modified MS medium. Mesophyll parenchymatous cells originated meristemoids (preembryonic complex formation induced with 7.1 µM BAP in the first two subculture periods. After polarized structures with 12.9 µM NAA and 3.55 µM BAP were formed in the third subculture, somatic embryos developed and regenerated normal plants. The mesophyll parenchymatous cells display high capacity of direct response to the auxin and cytokinin.O objetivo deste trabalho foi avaliar um protocolo de regeneração de plantas por meio de embriões somáticos, obtidos a partir de primórdios foliares de pupunheiras adultas e identificar a origem histológica dos embriões e descrever as etapas morfogenéticas. Os explantes foram cultivados em meio MS modificado. Células parenquimáticas do mesofilo originaram meristemóides com BAP (7,1 µM nos dois primeiros períodos de subcultura. A polarização das estruturas ocorreu com ANA (12,9 µM e BAP (3,55 µM no terceiro período de subcultura. Meristemóides se desenvolveram em embriões somáticos, regenerando plantas normais. As células parenquimáticas do mesofilo apresentam elevada capacidade de resposta direta à auxina e à citocinina.

  3. BAP updated guidelines: evidence-based guidelines for the pharmacological management of substance abuse, harmful use, addiction and comorbidity: recommendations from BAP.

    Science.gov (United States)

    Lingford-Hughes, A R; Welch, S; Peters, L; Nutt, D J

    2012-07-01

    The British Association for Psychopharmacology guidelines for the treatment of substance abuse, harmful use, addiction and comorbidity with psychiatric disorders primarily focus on their pharmacological management. They are based explicitly on the available evidence and presented as recommendations to aid clinical decision making for practitioners alongside a detailed review of the evidence. A consensus meeting, involving experts in the treatment of these disorders, reviewed key areas and considered the strength of the evidence and clinical implications. The guidelines were drawn up after feedback from participants. The guidelines primarily cover the pharmacological management of withdrawal, short- and long-term substitution, maintenance of abstinence and prevention of complications, where appropriate, for substance abuse or harmful use or addiction as well management in pregnancy, comorbidity with psychiatric disorders and in younger and older people.

  4. Regeneração in vitro de Parapiptadenia rigida In vitro regeneration of Parapiptadenia rigida

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    Paula Kielse

    2009-07-01

    Full Text Available O objetivo deste trabalho é estabelecer um protocolo de micropropagação para a Parapiptadenia rigida utilizando explantes de plântulas assépticas obtidas da germinação de sementes in vitro. Para a indução de brotos, segmentos cotiledonares e nodais foram inoculados em meio de cultura ½ WPM acrescido de 0; 0,25; 0,50; 1,0mg L-1 de BAP ou KIN. O enraizamento dos explantes com broto foi realizado em meio ¼ WPM suplementado com 0; 0,25; 1,0; 1,75mg L-1 de AIB. Não foi necessária a adição de citocinina ao meio para indução de brotos em segmento cotiledonar e nodal. Contudo, a dose de 0,50mg L-1, independente do tipo de citocinina, promoveu maior tamanho dos brotos e maior número de folhas por broto. O maior potencial de enraizamento ocorreu em segmentos cotiledonares inoculados em meio contendo 1,0mg L-1 de AIB. A utilização desse protocolo de regeneração permite a obtenção de mudas de Parapiptadenia rigida.The aim of this research is to develop a micropropagation protocol for Parapiptadenia rigida using aseptic seedling explants from in vitro germinated seeds. To the sprout induction, cotyledonary and nodal segments were inoculated in a ½ WPM culture medium containing 0; 0.25; 0.50; 1.0mg L-1 of BAP or KIN. The rooting of sprouted explants was rooted in a ¼ WPM culture medium containing 0; 0.25; 1.0; 1.75mg L-1 of IBA. It was not necessary the addition of cytokinin in medium to induce sprouting in cotyledonary and nodal segments. However, the doses of 0.50mg L-1, independently of cytokinin type, promoted greater size of sprouts and the largest number of leaves per sprout. The highest rooting potential occurred in cotyledonary segments inoculated in medium with 1.0mg L-1 of IBA. The use of this regeneration protocol allows obtaining Parapiptadenia rigida seedlings.

  5. Biotechnology action programme BAP 1985-1989. Progress report 1987. Vol. 1. An overview

    Energy Technology Data Exchange (ETDEWEB)

    Magnien, E. (ed.)

    1987-01-01

    The multiannual research and training programme of the European Economic Community in the field of biotechnology is presented. It deals with two essential tasks, namely the establishment of a supportive infrastructure for biotechnology research and development in Europe as well as the exploitation by industry and agriculture of the materials and methods originating from modern biology. (EF)

  6. Biotechnology action programme BAP 1985-1989. Progress report. Vol. 2. Scientific reports from participating laboratories

    Energy Technology Data Exchange (ETDEWEB)

    Magnien, E. (ed.)

    1987-01-01

    The present progress report has been prepared after the programme has been running for one and a half years. It outlines, in a condensed form, the preliminary results obtained during 1986 and the first semester of 1987 from transnational cost-shared research contracts between the Commission and associated public or private institutions from different Member States. With 105 figs., 26 tabs..

  7. Preparation and Physical Properties of One-Dimensional Structures: Bap(Fe2S4)q.

    Science.gov (United States)

    Swinnea, J. S.; Steinfink, H.

    1980-01-01

    Reviews the structure, preparation, and physical properties of a series of compounds characterized by structures formed from sublattices which have incommensurate repeat distances in one or two directions. (Author/CS)

  8. CSL推出电池辅助 远距离无源BAP ID卡

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Convergence Systems Limited(CSL),一个被动RFID标签,阅读器,天线,和有源设备的全球供应商,推出了他们的电池辅助无源(BAP)ID卡。BAPID是一种远距离识别卡,有长达半径25米(80英尺)的活跃距离,以满足要求苛刻的应用,如笔记本电脑资产跟踪,自动时间和出勤记录,学生证,危险的远程工作人员监测,免提访问控制,或动物跟踪等等。

  9. Meios de cultura, reguladores de crescimento e fontes de nitrogênio na regulação da calogênese do pau-brasil (Caesalpinia echinata Lam. Culture media, growth regulators and nitrogen sources in callus formation regulation of Brazilwood (Caesalpinia echinata Lam.

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    Elias Terra Werner

    2010-12-01

    Full Text Available A regulação da calogênese é o primeiro passo para a embriogênese somática indireta in vitro. Nesse sentido, foram investigados os efeitos de diferentes meios de cultura, reguladores de crescimento e fontes nitrogenadas no desenvolvimento de calos em tecidos foliares de Caesalpinia echinata (pau-brasil. Explantes foliares foram cultivados em meios de cultura MS, B5, White e WPM. Testou-se, também, o efeito dos compostos nitrogenados (NH4NO3, KNO3 e glutamina e a interação entre auxinas (2,4-D, AIA e AIB e citocininas (BAP e KIN na desdiferenciação celular indicado pela formação de calos. Os meios MS, B5 e White não foram significantemente diferentes e proporcionaram melhores resultados. Em meio MS contendo as fontes nitrogenadas 2,4 g L-1 NH4NO3 e 1,35 g L-1 glutamina + 4,11 g L-1 de KNO3 na presença de 1,0 mg L-1 2,4-D e 5,0 mg L-1 BAP ocorreu maior proliferação celular indicada pela massa fresca dos calos. As combinações dos reguladores 0,5 mg L-1 2,4-D + 5 mg L-1 BAP e 0,5 mg L-1 AIB + 5,0 mg L-1 BAP suplementado com 2,4 g L-1 NH4NO3 em meio MS estimularam o crescimento dos calos. De maneira geral, os calos apresentaram aspectos friáveis, não embriogênicos, com acúmulo de fenóis e presença de áreas meristemáticas (meristemóides.The regulation of callus formation is the first step to indirect somatic embryogenesis in vitro. Therefore, we investigated hormonal and nutrient factors on development of callus in leaf tissues of Caesalpinia echinata (Brazilwood. Explants were cultivated in culture media MS, B5, WPM, and White. The effect of nitrogen sources (NH4NO3, KNO3 and glutamine and the interaction between auxins (2,4-D, IAA and IBA and cytokinins (BAP and KIN were also evaluated. Media MS, B5 and White were not significantly different and provided better results. MS medium containing 2.4 g L-1 NH4NO3, 1.35 g L-1 glutamine, and 4.11 g L-1 of KNO3 in the presence of 1 mg.L-1 2,4-D and 5 mg.L-1 BAP increased cellular

  10. Organogênese a partir de segmentos foliares e internodais de videira cv. Merlot Organogenesis from leaf segments and internodes of grapevine cv. Merlot

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    Dayse Cristina de Carvalho

    2011-02-01

    Full Text Available Objetivou-se, neste trabalho, estudar a indução da organogênese em segmentos foliares e internódios de videira cv. Merlot, utilizando diferentes tipos e concentrações de citocininas. O meio de cultura usado foi o MS com redução da metade dos sais e vitaminas, suplementado com 1 µM de ácido naftalenoacético (ANA. Os tratamentos consistiram das concentrações de 2,5; 5,0 ou 10 µM de 6-benzilaminopurina (BAP ou 1-fenil-3-(1,2,3,-tiadiazol-5-il uréia (TDZ. Foram avaliados o porcentual de formação de gemas, calos, oxidação e raízes, além do número de raízes e gemas. Aos 60 dias de cultivo in vitro foi observado que concentrações de 2,5-10 µM de BAP e TDZ permitem que segmentos foliares oxidem e não induzam à oxidação nos internódios. O nível de 5 µM de TDZ favore o maior porcentual de formação de gemas em internódios e segmentos foliares não apresentam capacidade para formar gemas nos níveis de 2,5-10 µM de BAP ou TDZ. O uso de BAP nas concentrações de 2,5 e 5 µM favorece altos porcentuais de enraizamento em internódios. O uso de TDZ no nível de 2,5 µM promove elevada taxa de formação de calos em internódios. Os internódios apresentaram maior capacidade organogênica do que os segmentos foliares.The objective of this work was study the induction of organogenesis on leaf segments and internodes of grapevine cv. Merlot using different types and concentrations of cytokinins. MS half strength culture medium supplemented with 1 µM naftalenoacetic acid (NAA was used. The treatments consisted of the concentrations of 2.5; 5.0 or 10 µM 6-benzilaminopurine (BAP or thydiazuron (TDZ. The percentage of buds, callus, oxidation and roots as well as the number of roots and buds were evaluated. After 60 days of in vitro culture it was observed that the concentrations of 2.5-10 µM BAP or TDZ oxidize leaf segments and do not induce oxidation in the internodes. The level of 5 µM TDZ promote the largest formation of

  11. Multiplicação in vitro dos porta-enxertos de Prunus sp. 'Barrier' e 'Cadaman' In vitro multiplication of 'Barrier' and 'Cadaman' Prunus sp. rootstocks

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    Marcelo Couto

    2004-04-01

    Full Text Available Este trabalho foi realizado com o objetivo de estabelecer a melhor concentração de sais do meio MS e da citocinina BAP para a multiplicação dos porta-enxertos de Prunus sp. 'Barrier' e 'Cadaman'. Segmentos nodais foram introduzidos em tubos de ensaio contendo 10 mL de meio de cultura com variações na concentração de sais (MS; ½MS; e 2/3MS combinadas com cinco concentrações de BAP (0; 1,5; 2,5; 3,5 e 4,5 miM. Utilizou-se um fatorial 2x3x5, distribuído em blocos casualizados, compostos por quatro repetições contendo cinco tubos de ensaio cada uma, sendo inoculado um segmento nodal por tubo. As avaliações foram realizadas após cinco semanas de cultivo em ambiente com intensidade luminosa de 20 miE m-2 s-1, fotoperíodo de 16 horas e temperatura de 24 ± 4ºC. Verificou-se maior número médio de gemas e de brotações para a cultivar Barrier. À medida que se reduziu a concentração de sais do meio de cultura, obteve-se maior número de brotações, porém com menor tamanho. As regressões polinomiais das variáveis número de gemas, brotações por explante e comprimento das brotações apresentaram um ajustamento quadrático para níveis de BAP, atingindo os pontos de máximo 31,2 gemas/explante; 4,6 brotações por explante, e 8,1 mm de comprimento nas concentrações 3,3; 3,1, e 3,1 miM de BAP, respectivamente.The objective of this work was to establish the best salt and BAP concentration in MS medium for multiplication of 'Barrier' and 'Cadaman' Prunus sp. rootstocks. Nodal segments were introduced in test tubes with 10 mL of MS medium supplemented with different salt concentrations (MS; ½MS; and 2/3MS arranged with five BAP concentrations (0; 1.5; 2.5; 3.5; and 4.5 muM. It was applied a factorial 2x3x5, in a complete randomized block design with four replications composed by five test tubes each one. It was inoculated one nodal segment for each test tub. The evaluations was carried out after five weeks in room culture with

  12. Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis Utilização de técnicas de cultura de tecidos na proliferação de brotos e enraizamento de Calendula officinalis

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    Cristiane Pimentel Victório

    2012-09-01

    N reduzido à metade das concentrações de NH4NO3 e KNO3, para verificar o efeito de diferentes tipos de explantes (basal, intermediário e apical, meio contendo areia de praia como suporte em substituição do agar, e o efeito de citocininas e auxinas (TDZ, tidiazuron; BAP, 6-benzilaminopurina; AIA, ácido indol-3-acético; AIB, ácido indol-3-butírico; ANA, ácido naftaleno acético no desenvolvimento vegetal in vitro. Os resultados mostraram pronunciado enraizamento a partir dos explantes apicais, e também maior alongamento dos brotos e número de folhas. O meio sólido foi mais apropriado para as culturas de C. officinalis. A proliferação de brotos mostrou-se dependente de citocininas, com melhores resultados sob efeito de TDZ ou BAP em relação aos outros tratamentos. As plantas regeneradas a partir do meio contendo TDZ apresentaram aspecto vitrificado e deformações na morfogênese. A maior taxa de enraizamento (80% foi obtida utilizando AIA 0.1 mg L-1. Através da propagação in vitro foram obtidas plantas de C. officinalis saudáveis e com raízes, capazes de serem aclimatizadas, e permitindo contínuo suplemento de matéria-prima.

  13. In vitro organogenesis in some citrus species Organogênese in vitro em algumas espécies de cítrus

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    Evandro Henrique Schinor

    2011-06-01

    de Cítrus foi estudada para os genótipos Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana e C. aurantium, utilizando-se de segmentos de epicótilo como explante, cultivados em meio de cultura MT suplementado com diferentes concentrações de 6-benzilaminopurina (BAP - 0,0; 0,5; 1,0; 1,5 ou 2,0 mg L-1. Para os genótipos recalcitrantes C. limonia e C. aurantium, a organogênese in vitro também foi estudada, utilizando-se de segmento internodal como explante, cultivado em meio de cultura MT suplementado com 0; 0,5; 1,0; 2,0 ou 4,0 mg L-1 de BAP. A eficiência da suplementação do meio de cultura com a combinação de BAP (0,0; 1,0 ou 2,0 mg L-1 e NAA (0,0; 0,3 ou 0,5 mg L-1 no desenvolvimento de gemas adventícias foi avaliada para C. aurantium. Pode-se verificar que, nos quatro genótipos estudados, a suplementação do meio de cultura com BAP não é essencial para o desenvolvimento de gemas adventícias, quando segmentos de epicótilo são utilizados como explantes. De maneira geral, a suplementação do meio de cultura com BAP diminuiu a porcentagem de explantes responsivos, exceto para C. sinensis cv. 'Natal' e C. limonia, quando as concentrações de 1,5 e 2,0 mg L-1 foram utilizadas. Para C. limonia e C. aurantium, quando segmento internodal foi utilizado como explante, a presença de citocinina em concentrações até 2,0 mg L-1 estimulou a organogênese in vitro. No caso específico de C. aurantium, não ocorreu o desenvolvimento de gemas adventícias em explantes (segmento internodal cultivados em meio de cultura basal, sem a presença de BAP. Embora não tenham sido detectadas diferenças estatísticas significativas, a suplementação do meio de cultura com a combinação BAP e NAA favoreceu o desenvolvimento de gemas adventícias em C. aurantium. A melhor concentração de NAA variou conforme a concentração de BAP utilizada. Pela análise dos resultados obtidos, pode-se verificar que a eficiência da organogênese in vitro de Cítrus depende

  14. Indução de calos em explantes foliares de Murici-pequeno (Byrsonima intermedia A. Juss. Calli induction from leaf explants of murici-pequeno (Byrsonima intermedia A. Juss.

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    Raírys Cravo Nogueira

    2007-04-01

    Full Text Available O murici-pequeno (Byrsonima intermedia A. Juss. é um arbusto do cerrado cujo chá da casca do caule apresenta atividade adstringente nas diarréias e disenterias. O gênero Byrsonima apresenta taxa de germinação baixa e emergência lenta da plântula, dificultando a propagação sexuada. Objetivou-se com este trabalho obter calos friáveis em explantes foliares de murici-pequeno para estudos futuros em suspensão celular e metabolismo secundário, assim como embriogênese somática. Avaliou-se o efeito de diferentes concentrações da auxina 2,4-D e a sua interação com as citocininas TDZ e BAP na calogênese. Os resultados demonstraram que na ausência de 2,4-D não ocorre formação de calo em explantes foliares. A adição de BAP ou TDZ não influencia o processo de calogênese. Para a indução e proliferação de calo em explantes foliares de murici-pequeno, os resultados recomendam o uso de meio MS, acrescido de 1,0 mg L-1 de 2,4-D, mantendo os explantes em condição de escuro por 30 dias.Byrsonima intermedia A. Juss is a shrub of cerrado whose husk presents medicinal activities in diarrheas and dysenteries. The Byrsonima genus present low germination rate and slow plantlet emergency which makes difficult sexual propagation. The objective of this work was to obtain friable callus in leaf explants for future studies with suspension cells and secondary metabolites as well as somatic embryogenesis. The effect of different concentrations of 2,4-D and its interaction with TDZ and BAP in callus formation was evaluated. The results demonstrated that there is no formation of callus in leaf explants maintained in absence of 2,4-D. The addition of TDZ or BAP had no influence in the calogenesis process. For callus induction and proliferation, the results suggest the use of MS medium supplemented with 1.0 mg L-1 2,4-D maintaining the explants in the dark.

  15. Solasodine accumulation in regenerated plants of Solanum torvum Sw Acúmulo de solasodina em plantas micropropagadas de Solanum torvum Sw

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    C.B Moreira

    2010-03-01

    Full Text Available A nodal segment culture was developed in order to assess Solanum torvum Sw. regeneration and solasodine levels. The influence of auxins (indoleacetic acid, 1-Naphthaleneacetic acid and benzyl adenine on S. torvum growth in micropropagation was investigated. A nodal segment culture was initiated with seeds germinated in MS basal medium added of GA3 and grown in different concentrations of IAA, IAA + BAP and NAA + BAP. Sixty-day-old plants from the in vitro culture were collected, frozen and lyophilized; then, the methyl orange method was used to quantify solasodine for the spectrophotometric assay. The best results regarding plant regeneration and solasodine accumulation were obtained by using the MS basal medium without addition of plant growth regulators; however, there was great production of calluses presenting friable bases. Based on these results, cell cultures can be initiated from such calluses with application of other auxins and cytokinins to enhance solasodine production, besides different elicitors, light intensities and sucrose concentrations.A regeneração de Solanum torvum e a avaliação do conteúdo de solasodina foram os objetivos de cultura de segmentos nodais. A influência de auxinas (ácido 3-indolacético, ácido naftalenoacético e de 6-benzilaminopurina no crescimento de S. torvum na micropropagação foi investigado. Cultura de segmentos nodais foi iniciada por sementes germinadas em meio básico MS acrescido de GA3 e cultivadas em diferentes concentrações de AIA, AIA + BAP e ANA + BAP. Plantas da cultura in vitro com 60 dias foram coletadas, congeladas e liofilizadas e o método de alaranjado de metila foi utilizado para quantificação de solasodina para o ensaio espectrofotométrico. Os melhores resultados para regeneração vegetal e acúmulo de solasodina foram alcançados no meio MS sem adição de reguladores de crescimento havendo, porém grande produção de calos de base friáveis. Esses resultados mostram

  16. Efeito de 6-benzilaminopurina sobre a propagação in vitro de Mimosa caesalpiniifolia Benth. (Fabaceae

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    Rafaela Maria de França Bezerra

    2014-10-01

    Full Text Available A Mimosa caesalpiniifolia, popularmente conhecida como sabiá, é uma espécie nativa da Caatinga que, em razão de suas inúmeras potencialidades, tem enfrentado um processo de exaustiva exploração, tornando iminente a necessidade de se utilizar alternativas sustentáveis que permitam a sua reposição em ambiente natural e a conservação de seu genótipo. A micropropagação tem sido considerada técnica promissora nesse sentido, pois viabiliza a produção de mudas em larga escala e com elevada sanidade. Citocininas como a 6-benzilaminopurina (BAP são importantes nesse processo, pois influenciam consideravelmente o crescimento e a morfogênese in vitro e permitem a formação de bancos de germoplasma in vitro. O objetivo deste trabalho foi verificar a influência de concentrações de BAP sobre a indução de brotações in vitro em M. caesalpiniifolia. Segmentos cotiledonares obtidos de plântulas germinadas in vitro foram inoculados em meio de cultura WPM suplementados com seis concentrações de BAP: 0,0; 4,44; 8,88; 17,76; 26,64; e 35,52 µmol/L. O delineamento experimental utilizado foi o inteiramente casualizado com seis tratamentos, três repetições e cada repetição composta por 10 unidades experimentais. Aos 30 dias, foram avaliados o número de explantes responsivos, o número de brotos por explante e a presença de calos, oxidação e contaminação. Os dados foram avaliados estatisticamente através da Análise de Correspondência e mediante o ajuste de Equações de Regressão. Verificou-se que a concentração de 17,76 µmol/L apresentou-se mais responsiva em relação à taxa de multiplicação e ao número de brotações, sendo, portanto, a concentração mais indicada para a propagação in vitro de M. caesalpiniifolia.

  17. Seasonal variation for the ratio of BaP to BeP at different sites in Great Xiamen Bay.

    Science.gov (United States)

    Wu, Shui-Ping; Qian, Ran-Ran; Lee, Tsung-Chang; Wang, Xing-Hong; Hong, Hua-Sheng; Yuan, Chung-Shin

    2012-04-01

    From March 2008 to February 2009, PM(10) samples were collected and analyzed for polycyclic aromatic hydrocarbons (PAHs) at eight sampling sites in Great Xiamen Bay, China. Analyses of the seasonal and spatial variations of these compounds revealed the following results. Significantly high levels of PAHs were found in the winter compared to the summer, sometimes exceeding 100 ng m(-3), and the spatial variations were influenced most by the sampling site surroundings. Composition profiles of PAHs of an urban and a rural site were shown to be very similar with a positive correlation coefficient larger than 0.9 at the 0.01 level of significance for the same season. Diagnostic ratios, together with principal component and multiple linear regression analysis, showed that more PAHs were from grass/wood/coal combustion in winter than in other seasons. The ratios of benzo[a]pyrene to benzo[e]pyrene (BaP-BeP) in winter and fall were 0.6-1.7 times higher than those in spring and summer, suggesting the importance of local emissions of PAHs. The BaP-BeP ratios in Kinmen were generally lower than those in Xiamen, indicating that the aging degree of PAHs was higher in Kinmen than in Xiamen. The external input of PAHs from upwind urban and industrial areas was one of the key factors causing high levels of PAHs in PM(10) in Great Xiamen Bay in winter.

  18. RF-components embedded with photonic-band-bap (PBG) and fishnet-metamaterial structures for high frequency accelerator application

    CERN Document Server

    Robak, Sara; Shin, Young-Min

    2015-01-01

    In the development of high efficiency and high gradient RF-accelerators, RF waveguides and cavities have been designed with Photonic Band Gap (PBG) and fishnet- metamaterial structures. The designed structures are comprised of a periodically corrugated channel sandwiched between two photonic crystal slabs with alternating high to low dielectric constants and a multi-cell cavity-resonator designed with fishnet-metamaterial apertures. The structural designs of our interest are intended to only allow an operating-mode or -band within a narrow frequency range to propagate. The simulation analysis shows that trapped non-PBG modes are effectively suppressed down to ~ -14.3 dB/cm, while PBG modes propagated with ~2 dB of insertion loss, corresponding to ~1.14 dB/cm attenuation. The pre- liminary modeling analysis on the fishnet-embedded cavity shows noticeable improvement of Q-factor and field gradient of the operating mode (TM010) compared to those of typical pillbox- or PBG-cavities. Fabrication of the Ka-band PBG...

  19. BAP guidelines on the management of weight gain, metabolic disturbances and cardiovascular risk associated with psychosis and antipsychotic drug treatment.

    Science.gov (United States)

    Cooper, Stephen J; Reynolds, Gavin P; Barnes, Tre; England, E; Haddad, P M; Heald, A; Holt, Rig; Lingford-Hughes, A; Osborn, D; McGowan, O; Patel, M X; Paton, C; Reid, P; Shiers, D; Smith, J

    2016-08-01

    Excess deaths from cardiovascular disease are a major contributor to the significant reduction in life expectancy experienced by people with schizophrenia. Important risk factors in this are smoking, alcohol misuse, excessive weight gain and diabetes. Weight gain also reinforces service users' negative views of themselves and is a factor in poor adherence with treatment. Monitoring of relevant physical health risk factors is frequently inadequate, as is provision of interventions to modify these. These guidelines review issues surrounding monitoring of physical health risk factors and make recommendations about an appropriate approach. Overweight and obesity, partly driven by antipsychotic drug treatment, are important factors contributing to the development of diabetes and cardiovascular disease in people with schizophrenia. There have been clinical trials of many interventions for people experiencing weight gain when taking antipsychotic medications but there is a lack of clear consensus regarding which may be appropriate in usual clinical practice. These guidelines review these trials and make recommendations regarding appropriate interventions. Interventions for smoking and alcohol misuse are reviewed, but more briefly as these are similar to those recommended for the general population. The management of impaired fasting glycaemia and impaired glucose tolerance ('pre-diabetes'), diabetes and other cardiovascular risks, such as dyslipidaemia, are also reviewed with respect to other currently available guidelines.These guidelines were compiled following a consensus meeting of experts involved in various aspects of these problems. They reviewed key areas of evidence and their clinical implications. Wider issues relating to primary care/secondary care interfaces are discussed but cannot be resolved within guidelines such as these.

  20. Induksi Tunas Tanaman Gaharu (Aquilaria malaccencis Lamk) dengan Menggunakan Kombinasi Zat Pengatur Tumbuh BAP dan IAA secara In Vitro

    OpenAIRE

    Anggriani, Fani

    2011-01-01

    Agarwood (Aquilaria malaccensis Lamk.) is one of the important tropical forest trees, which produces a high economically valuable fragrant resinous wood. The increase of agarwood demand from year to year leads to uncontrolled illegal harvest of this plant from its natural habitat. To encounter this problem, there is an urgent need to develop agarwood plantation. The pusrpose of this research is to determine of combination concentrate the best growth regulators for shoot induction of agarwood...

  1. BAP guidelines on the management of weight gain, metabolic disturbances and cardiovascular risk associated with psychosis and antipsychotic drug treatment.

    Science.gov (United States)

    Cooper, Stephen J; Reynolds, Gavin P; Barnes, Tre; England, E; Haddad, P M; Heald, A; Holt, Rig; Lingford-Hughes, A; Osborn, D; McGowan, O; Patel, M X; Paton, C; Reid, P; Shiers, D; Smith, J

    2016-08-01

    Excess deaths from cardiovascular disease are a major contributor to the significant reduction in life expectancy experienced by people with schizophrenia. Important risk factors in this are smoking, alcohol misuse, excessive weight gain and diabetes. Weight gain also reinforces service users' negative views of themselves and is a factor in poor adherence with treatment. Monitoring of relevant physical health risk factors is frequently inadequate, as is provision of interventions to modify these. These guidelines review issues surrounding monitoring of physical health risk factors and make recommendations about an appropriate approach. Overweight and obesity, partly driven by antipsychotic drug treatment, are important factors contributing to the development of diabetes and cardiovascular disease in people with schizophrenia. There have been clinical trials of many interventions for people experiencing weight gain when taking antipsychotic medications but there is a lack of clear consensus regarding which may be appropriate in usual clinical practice. These guidelines review these trials and make recommendations regarding appropriate interventions. Interventions for smoking and alcohol misuse are reviewed, but more briefly as these are similar to those recommended for the general population. The management of impaired fasting glycaemia and impaired glucose tolerance ('pre-diabetes'), diabetes and other cardiovascular risks, such as dyslipidaemia, are also reviewed with respect to other currently available guidelines.These guidelines were compiled following a consensus meeting of experts involved in various aspects of these problems. They reviewed key areas of evidence and their clinical implications. Wider issues relating to primary care/secondary care interfaces are discussed but cannot be resolved within guidelines such as these. PMID:27147592

  2. Multiplicação in vitro de abacaxi ornamental por estiolamento e regeneração de brotações In vitro multiplication of ornamental pineapple by shoot etiolation and regeneration

    Directory of Open Access Journals (Sweden)

    Ana Cristina PP de Carvalho

    2009-03-01

    Full Text Available Foi multiplicado in vitro, o abacaxi ornamental Ananas comosus var. erectifolius, induzindo o estiolamento de segmentos nodais com posterior regeneração de brotações. Os eixos caulinares foram inoculados nos meios de cultura: MS sem regulador de crescimento; MS + 10 µM de ácido indolbutírico (AIB; MS + 10 µM de ácido naftalenoacético (ANA; MS + 10 µM de ácido indolacético (AIA, e mantidos no escuro, a 25±2ºC. Aos 60 dias após a inoculação, avaliaram-se número de brotos/explante, número de nós/broto, comprimento de brotos, distância entre os nós e número total de nós/explante. O meio MS + 10 µM de ANA promoveu os maiores valores para número de brotos e número total de nós/explante. Para a regeneração de brotos, foram utilizados segmentos nodais, oriundos de eixo caulinar (explante estiolados in vitro, contendo dois nós, nos meios: MS sem regulador de crescimento; MS + 4,44 µM de 6-benzilaminopurina (BAP; MS + 8,88 µM de BAP e MS + 13,32 µM de BAP. As culturas foram incubadas sob fotoperíodo de 16 horas, a 25±2ºC. Aos 30 dias, não houve diferença no número de brotos regenerados por nó entre os meios testados, enquanto aos 45 e 60 dias de cultivo, a adição de BAP teve efeito positivo sobre o número de brotos regenerados por nó, quando comparado com o meio sem a adição desta citocinina.The in vitro multiplication of etiolated nodal segments was evaluated for Ananas comosus var. erectifolius shoots production. Stems were inoculated in the following media: MS without growth regulator; MS + 10 µM of indolbutiric acid (IBA; MS + 10 µM of naphtalenacetic acid (NAA; MS + 10 µM of indolacetic acid (IAA; and stored in darkness at 25±2ºC. 60 days after inoculation, the number of etiolated shoots/stem; number of nodes/etiolated shoot; etiolated shoot length, internode length and total number of nodes/stem were evaluated. The medium MS + 10 µM of NAA showed the highest values of number of etiolated shoots

  3. Protocolo de micropropagação da goiabeira serrana (Acca sellowiana (Berg Burret Feijoa (Acca sellowiana (Berg Burret micropropagation protocol

    Directory of Open Access Journals (Sweden)

    Ana Carla Oltramari

    2000-03-01

    Full Text Available Visando ao desenvolvimento de um novo protocolo para a micropropagação da goiabeira serrana (Acca sellowiana (Berg Burret foram estabelecidos experimentos com segmentos nodais e microestacas. As citocininas 6-Benzilaminopurina (BAP, Cinetina (Kin e 2-Isopenteniladenina (2-iP foram adicionadas ao meio de cultura Woody Plant Midium-WPM, visando à proliferação de brotações múltiplas dos genótipos 53B-7, 101, 529 e 152-12 x 458. As microestacas obtidas in vitro foram submetidas a diferentes períodos de indução, em 20 miM de ácido indolbutírico (AIB e, posteriormente, transferidas para meio de cultura isento desse fitorregulador para a indução de raízes ou, alternativamente, submetidas a diferentes concentrações e tempos de exposição em AIB e transferidas para substrato. As citocininas empregadas não promoveram aumento na taxa de proliferação de brotos em relação à testemunha. O meio de cultura basal WPM, adicionado de Kin (5 miM, proporcionou maior altura média dos brotos. Segmentos nodais do acesso 101 cultivados em meio de cultura WPM, isento de fitorreguladores, apresentaram maiores taxas médias de proliferação. Pulsos de seis dias com AIB (20 miM induziram uma maior taxa de enraizamento (68,9%, um maior número médio de raízes (1,3 raízes e raízes com maior comprimento médio (5,6miM. Microestacas enraizadas ex vitro, pela imersão em AIB (100 miM por 60 minutos, resultaram em maior altura das plantas (45,3mm, número de raízes secundárias (11,3 raízes, massa fresca (1069mg e seca das raízes (282mg.The objective of this study was to establish a micropropagation protocol for Acca sellowiana (Berg Burret. Nodal segments of in vitro cultivated plantlets of the genotypes 53B-7, 101, 529 and 152-12 x 458 were inoculated in test tubes containing 15ml of Woody Plant Medium-WPM solid medium supplemented with the cytokinins BAP, Kin, and 2-iP. For rooting purpose the microcuttings were submitted to different

  4. Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha Micropropagação das bromélias ornamentais vulneráveis Aechmea blanchetiana e Aechmea distichantha

    Directory of Open Access Journals (Sweden)

    Sandra Santa-Rosa

    2013-03-01

    élias nativas do Brasil, de grande valor ornamental. Não sendo produzidas comercialmente, ambas sofrem exploração predatória. Este trabalho teve como objetivos (1 avaliar a germinação de sementes in vitro de A. blanchetiana e A. distichantha e (2 estabelecer protocolos de micropropagação destas bromélias, visando a produção de mudas para atender ao mercado e à conservação in vitro. Os experimentos de germinação foram conduzidos em delineamento inteiramente casualizado, com dois tratamentos (presença e ausência de luz e sete e oito repetições respectivamente para A. blanchetiana e A. distichantha. Plantas in vitro, obtidas em presença de luz, foram utilizadas como explantes no experimento de multiplicação (meio MS. O delineamento utilizado foi inteiramente casualizado, em fatorial 2 (concentrações de ANA: 0,05 e 0,5 μM x 2 (citocininas: BAP e CIN x 2 (concentrações de citocininas: 2,2 e 4,4 μM + 1 (controle com meio MS básico, sem reguladores de crescimento, com 10 repetições por tratamento. Foram avaliadas as contaminações fúngica e bacteriana, germinação total e cumulativa e número de brotos por tratamento em cada subcultivo. Observou-se apenas contaminação fúngica e somente em A. blanchetiana (14,0%. A presença de luz favoreceu a germinação de sementes in vitro de ambas as espécies (99 e 62% para respectivamente A. blanchetiana e A. distichantha. Meio MS + 0,5 μM ANA + 2,2 μM BAP promoveu a maior taxa de multiplicação para ambas as espécies, com contrastes significativos entre este tratamento e o controle em ambas as espécies (105,62 brotos a mais em A. blanchetiana; 223,80 em A. distichantha. BAP promoveu a formação de brotos sem raízes, enquanto cinetina favoreceu a formação de raízes em detrimento de brotos. Na aclimatização das plântulas, A. blanchetiana e A. distichantha apresentaram 90 e 97% de sobrevivência respectivamente.

  5. Multiplicação in vitro de porta-enxertos do gênero Prunus sob baixas concentrações e diferentes tipos de auxinas

    Directory of Open Access Journals (Sweden)

    Silveira Carlos Augusto Posser

    2002-01-01

    Full Text Available Experimentos testando o tipo de auxina (AIB, AIA e ANA e concentração (0; 0,01; 0,1 e 1,0 mM foram realizados para cada um dos quatro porta-enxertos utilizados (G x N22, Mr.S 2/5, Marianna e Mirabolano. Cada porta-enxerto constituiu-se num experimento, uma vez que, apesar de ser o mesmo meio básico, MS fraction three-quarters, com exceção de Marianna, onde o meio foi o MS, as concentrações da citocinina BAP foram diferentes. A cada meio, foram adicionados os tipos e as concentrações das auxinas a serem testadas. Os porta-enxertos apresentaram comportamentos distintos quanto ao tipo e concentração das auxinas. O AIB e o AIA foram superiores ao ANA nas variáveis analisadas. Na fase de multiplicação, o 'Mirabolano' não respondeu a concentrações de auxinas de até 1,0 mM. O 'G x N22' apresentou as melhores respostas na concentração de 0,01 miM de AIB ou AIA. O porta-enxerto Marianna respondeu positivamente a concentrações de auxinas entre 0,01 e 1,0 miM. O ANA não proporciona bons resultados na multiplicação in vitro de porta-enxertos do gênero Prunus, sendo muitas vezes superado pela testemunha.

  6. Regeneração in vitro de urucum (Bixa orellana L. a partir de diferentes tipos de explantes In vitro regeneration of annatto (Bixa orellana L. from various explants

    Directory of Open Access Journals (Sweden)

    Jane Fiuza Rodrigues Portela de Carvalho

    2005-12-01

    Full Text Available Este trabalho teve como objetivo avaliar a regeneração in vitro de plantas de urucum (Bixa orellana L. a partir de diferentes tipos de explantes. Para definir o meio de cultura adequado para indução de brotações, diferentes concentrações e, ou, combinações da auxina AIA e das citocininas BAP e ZEA foram testadas. As melhores respostas de regeneração para segmentos de hipocótilo, nós cotiledonares e hipocótilos invertidos foram observadas em meios suplementados de ZEA (2,28 µM e AIA (0,30 µM, ZEA (4,56 µM e ZEA (4,56 µM, respectivamente. O meio de enraizamento mais eficaz foi o MS, com a metade de sua concentração salina e 5 µM de AIB. Análises citológicas, realizadas antes da aclimatação, confirmaram a estabilidade cromossômica das plantas cultivadas in vitro, não sendo detectado variação com relação ao número de cromossomos metafásicos (2n = 14.The present work aimed the establishment of a regeneration protocol for annatto (Bixa orellana L. from different juvenile explants. In order to promote shoot induction, different concentrations and/or combinations of IAA and the cytokinins BAP and ZEA were assessed. Better regeneration responses were achieved when segmented hypocotyl, cotiledonary nodes and inverted hypocotyl were cultured onto MS-based medium supplemented with ZEA (2.28 µM and IAA (0.30 µM, ZEA (4.56 µM or ZEA (4.56 µM, respectively. Rooting of elongated shoots displayed higher frequencies when half-strength MS medium with IBA (5 µM was used. No genetic variation was detected among regenerants as revealed by cytological analysis based on metaphasic chromosome countings (2n = 14.

  7. Micropropagação de Croton antisyphiliticus Mart. Micropropagation of Croton antisyphiliticus Mart.

    Directory of Open Access Journals (Sweden)

    Taíce Gonçalves de Oliveira

    2011-10-01

    Full Text Available Croton antisyphiliticus Mart. ex M. Arg., conhecido popularmente como pé-de-perdiz, é uma planta medicinal nativa do Cerrado, cuja raiz é utilizada na forma de decoctos para combater infecções do aparelho reprodutor masculino e feminino. A coleta da planta é realizada de forma extrativista e não há trabalhos a respeito da sua conservação. O objeto do trabalho foi estabelecer um protocolo de micropropagação de Croton antisyphiliticus. Foram avaliados o efeito de diferentes concentrações das citocininas, o tamanho do recipiente para o cultivo in vitro, o co-cultivo e a posição da gema em relação ao desenvolvimento dos explantes, bem como a influência do AIB no enraizamento in vitro e a aclimatização dos explantes. O melhor desenvolvimento in vitro foi obtido com explantes do tipo gema apical, co-cultivadas em meio MS suplementado com BAP (1µM, em frasco tipo pote. O enraizamento foi obtido em meio MS sem regulador vegetal e a aclimatização pode ser realizada em solo do cerrado com brotações sem a presença de raízes desenvolvidas in vitro.Croton antisyphiliticus Mart. ex M. Arg., popularly known as "pé-de-perdiz", is a native medicinal plant of Cerrado, whose root is used in the form of decoctions against infections of the male and female reproductive. The collection of the plant is carried through of extractives form and there are no studies regarding its conservation. The aim of this study was to establish a micropropagation protocol of C. antisyphiliticus. The effect of different concentrations of cytokinins, the size of the container for the in vitro culture, co-cultivation and bud position in relation to the development of the explants, as well as the influence of AIB on in vitro rooting and acclimatization of explants were evaluated. The best development in vitro was obtained with apical explants co-cultivated on MS medium supplemented with BAP (1µM in bottle type pot. Rooting was achieved on MS medium without

  8. In vitro organogenesis in watermelon cotyledons Organogênese in vitro em cotilédones de melancia

    Directory of Open Access Journals (Sweden)

    Maria Graziela Zagatto Krug

    2005-09-01

    Full Text Available The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L-1 and coconut water (10%. The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants. Adventitious shoots were characterized by the development of shoot apical meristem and leaf primordia. The formation of protuberances, that do not develop into adventitious buds, was also observed.O objetivo do trabalho foi estudar a organogênese in vitro de C. lanatus, pela indução de gemas adventícias, em segmentos de cotilédones, cultivados em meio de cultura suplementado com citocinina. Os explantes consistiram de segmentos das regiões distal e proximal de cotilédones, coletados de plantas germinadas in vitro com um, três e cinco dias de idade. Os dados obtidos mostram que a organogênese de melancia, in vitro, ocorre com maior eficiência em segmentos da região proximal dos cotilédones, coletados de plântulas com três dias de idade e cultivados em meio de cultura MS, suplementado com a combinação BAP (1 mg L-1 e água de coco (10%. Pelo estudo histológico, verificou-se que a organogênese ocorre diretamente, sem a formação de calo, na epiderme e subepiderme do explante. As gemas adventícias foram caracterizadas pela presença de meristema apical e primórdios foliares. Observou-se, também, o desenvolvimento de protuberâncias que não se desenvolvem em gemas adventícias.

  9. Propagação in vitro e caracterização química do óleo essencial de Lavandula angustifolia cultivada no Sul do Brasil In vitro propagation and chemical characterization of the essential oil of Lavandula angustifolia cultivated in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Marília Pereira Machado

    2013-02-01

    Full Text Available Lavandula angustifolia Mill. é uma importante planta produtora de óleos essenciais, que apresenta dificuldade de propagação pelo enraizamento de estacas, sendo a produção de mudas realizada nesse trabalho pela técnica de micropropagação, em que foram avaliadas algumas condições do cultivo in vitro. As mudas produzidas pela micropropagação foram cultivadas em São Joaquim (SC. O óleo essencial das inflorescências foi extraído por hidrodestilação, e analisado quanto a sua composição por GC/MS, em dois anos de colheita. O meio de cultura LS apresentou melhor resposta no desenvolvimento das brotações, não sendo observadas brotações com necrose apical. Meios de cultura mais diluídos como o ½ MS e o WPM, apresentaram altas taxas de hiperidricidade e necrose apical das brotações. A citocinina BAP influenciou a multiplicação das brotações, sendo a qualidade mantida com 1,0µM ao longo de quatro subcultivos. A sobrevivência das plantas na aclimatização foi de 80%. Os teores de óleo essencial foram de 4,0% e 5,25%, na primeira e segunda colheita, respectivamente. Os compostos majoritários foram o linalol (46,88% e 37,25% e acetato de linalila (10,09% e 12,24%.Lavandula angustifolia Mill. is an important producer of essential oil, which presents the difficulty of propagation stem cuttings. In this work the propagation of plant was carried out by micropropagation technique, in which certain conditions were evaluated in vitro. The micropropagated plants were grown in São Joaquim (SC. The essential oil of the inflorescences was extracted by hydrodistillation and analyzed for their composition by GC/MS in two years of harvest. The culture medium LS showed a better response in the development of shoots. Culture media more diluted as the 1/2 MS and WPM showed high rates of hyperhrydricity and apical necrosis. The BAP influenced the shoot multiplication and the quality being maintained with 1.0 mM over four subcultures. The

  10. Ação conjunta de citocinina, giberelina e auxina em pimentão enxertado e não enxertado sob cultivo protegido Joint action of cytokinin, gibberellin and auxin on grafted and non-grafted sweet pepper under protected cultivation

    Directory of Open Access Journals (Sweden)

    Felipe C Palangana

    2012-12-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da utilização do bioestimulante Stimulate® em plantas de pimentão enxertadas e não enxertadas, em ambiente protegido, no aumento de produção. Utilizou-se o delineamento experimental de blocos casualizados, no esquema fatorial 2 x 6, referente a plantas enxertadas e pé-franco e seis doses do Stimulate® (0; 75; 100; 125; 150 e 175 mL p.c. 100 L H2O-1, com quatro repetições. Realizaram-se sete aplicações foliares, quinzenais, do bioestimulante a partir do início do florescimento das plantas e avaliou-se o número total de frutos comerciais produzidos, comprimento, calibre, espessura de parede, massa média e produtividade final, totalizando treze colheitas. As plantas enxertadas foram mais produtivas em relação às plantas pé-franco, produzindo frutos de características agronômicas superiores. No geral, 100 e 125-150 mL p.c. 100 L H2O-1 de Stimulate® promovem incremento na produção de pimentão enxertado e pé-franco, respectivamente.The objective of this study was to evaluate the effect of using the Stimulate® biostimulant in grafted and non grafted plants of sweet pepper in a protected environment, to increase production. The experimental design was randomized blocks in a factorial scheme 2 x 6, referring to the grafted and non grafted plants and six doses of Stimulate® (0; 75; 100; 125; 150 and 175 mL p.c. 100 L H2O-1, with four replications. Seven foliar applications of the biostimulant were carried out fortnightly, from the beginning of the plants flowering and the total number of marketable fruits, length, diameter, wall thickness, average weight and final yield were evaluated, totalling thirteen harvests. The grafted plants were more productive than the non-grafted ones, producing fruits with better agronomic characteristics. In general, 100 and 125-150 mL p.c. 100 L H2O-1 of Stimulate® promoted an increase in the production of grafted and non-grafted sweet pepper plants, respectively.

  11. Multiplicação in vitro do porta-enxerto de Prunus spp. 'Carelli' In vitro multiplication of Prunus spp. rootstocks 'Carelli'

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    Paulo de Tarso Teixeira

    2004-08-01

    Full Text Available No Brasil, a falta de porta-enxertos para as Prunáceas, principalmente de origem clonal, tem incentivado a seleção de novas variedades e o uso de técnicas de cultura in vitro para a propagação. Neste sentido, o objetivo deste trabalho foi avaliar o potencial de multiplicação in vitro do porta-enxerto 'Carelli' sob efeito de diferentes concentrações da citocinina 6-benzilaminopurina (BAP. Segmentos nodais com 0,5 cm de comprimento foram inoculados em meio de cultura de Lepoivre, suplementado com 0,0; 0,5; 1,0; 2,0 e 4,0 mg.L-1 BAP. Estes segmentos nodais são oriundos de plântulas preestabelecidas in vitro, após duas subculturas em meio de cultura de Lepoivre, suplementado com 0,5 mg.L-1 de BAP. As avaliações para número de brotos por explante e altura média das brotações foram realizadas após 21 dias de cultura in vitro. Os resultados mostraram que os tratamentos com BAP não apresentaram diferenças significativas entre si. A taxa média de multiplicação foi de 3,3 a 3,4 brotos por explante. O tratamento sem adição de BAP não apresentou a formação de brotações axilares, mas resultou em brotos com maior altura média (16,2 mm. O uso de BAP afetou significativamente a altura das brotações, e o acréscimo nas suas concentrações reduziu o comprimento das mesmas. Concentrações de BAP superiores a 1,0 mg.L-1de BAP reduziram o comprimento das brotações e promoveram hiperidricidade. O uso de 0,5 mg.L-1 de BAP promoveu a formação de 3,3 brotos por explante com 11,0 mm de altura média, em condições adequadas para o enraizamento.In Brazil the lack of well adapted rootstocks in the Peach industry, mainly of clonal origin, forces the selection of new varieties and the use of tissue culture techniques for the mass clonal propagation. In the present work it was evaluated the in vitro multiplication potential of "Carelli" rootstock in response to different levels of BA. Nodal segments (0.5 cm length were inoculated in

  12. Stochastic multi-scale prediction on the apparent elastic moduli of trabecular bone considering uncertainties of biological apatite (BAp) crystallite orientation and image-based modelling.

    Science.gov (United States)

    Basaruddin, Khairul Salleh; Takano, Naoki; Nakano, Takayoshi

    2015-01-01

    An assessment of the mechanical properties of trabecular bone is important in determining the fracture risk of human bones. Many uncertainty factors contribute to the dispersion of the estimated mechanical properties of trabecular bone. This study was undertaken in order to propose a computational scheme that will be able to predict the effective apparent elastic moduli of trabecular bone considering the uncertainties that are primarily caused by image-based modelling and trabecular stiffness orientation. The effect of image-based modelling which focused on the connectivity was also investigated. A stochastic multi-scale method using a first-order perturbation-based and asymptotic homogenisation theory was applied to formulate the stochastically apparent elastic properties of trabecular bone. The effective apparent elastic modulus was predicted with the introduction of a coefficient factor to represent the variation of bone characteristics due to inter-individual differences. The mean value of the predicted effective apparent Young's modulus in principal axis was found at approximately 460 MPa for respective 15.24% of bone volume fraction, and this is in good agreement with other experimental results. The proposed method may provide a reference for the reliable evaluation of the prediction of the apparent elastic properties of trabecular bone.

  13. 环境中苯并(a)芘监测技术及建议%Monitoring Technique and Suggestion on Monitoring BaP in Environment

    Institute of Scientific and Technical Information of China (English)

    陶大钧; 奚稼轩; 周圣东

    2001-01-01

    环境中BaP是引起人们各类癌症的主要诱发物.叙述了环境中BaP的来源及其浓度状况,列举了国家制定的各项标准中的BaP含量的浓度限值,论述了环境水中和空气中BaP的样品采集、保存、前处理、分析测定及定量监测中应注意的几个问题,提出了监测BaP的4点建议.

  14. The NASA/Baltimore Applications Project (BAP). Computer aided dispatch and communications system for the Baltimore Fire Department: A case study of urban technology application

    Science.gov (United States)

    Levine, A. L.

    1981-01-01

    An engineer and a computer expert from Goddard Space Flight Center were assigned to provide technical assistance in the design and installation of a computer assisted system for dispatching and communicating with fire department personnel and equipment in Baltimore City. Primary contributions were in decision making and management processes. The project is analyzed from four perspectives: (1) fire service; (2) technology transfer; (3) public administration; and (5) innovation. The city benefitted substantially from the approach and competence of the NASA personnel. Given the proper conditions, there are distinct advantages in having a nearby Federal laboratory provide assistance to a city on a continuing basis, as is done in the Baltimore Applications Project.

  15. EFEITO DO ARMAZENAMENTO E DE FITOHORMÔNIOS NA QUALIDADE FISIOLÓGICA DE SEMENTES DE ARATICUM (Annona crassiflora Mart. EFFECT OF STORAGE AND PLANT HORMONES IN GERMINATION OF ARATICUM SEEDS (Annona crassiflora Mart.

    Directory of Open Access Journals (Sweden)

    Marcos Antônio Machado Mesquita

    2007-10-01

    Full Text Available

    As sementes de araticum (Annona crassiflora Mart. apresentam germinação lenta e desuniforme, o que afeta negativamente a sua propagação. O experimento foi conduzido na Escola de Agronomia e Engenharia de Alimentos da Universidade Federal de Goiás, Goiânia-GO. Foram utilizadas sementes de araticum provenientes de árvores vegetando em estado natural, no nordeste de Goiás. Aleatoriamente, coletou-se um fruto maduro por árvore, de um total de cinqüenta plantas dispersas na região. Uma coleta foi realizada em março de 1999, cujas sementes foram armazenadas por 365 dias, sendo avaliadas após esta estocagem. Em março de 2000, foi realizada nova coleta em outras cinqüenta plantas, avaliando-se imediatamente as sementes recém-colhidas. Os tratamentos de superação de dormência foram: testemunha, água destilada, ácido giberélico à 1.000 ppm, citocinina à 1.000 ppm, GA3 + BAP à 500 ppm. A semeadura foi realizada em sacos de polietileno preto, contendo areia grossa lavada, terriço de mata e terra de subsolo (3:3:4. O armazenamento das sementes por um ano não proporcionou taxa adequada de germinação em nenhum dos tratamentos. O ácido giberélico à 1.000 ppm é eficiente na superação da dormência em sementes recém-colhidas. As sementes dessa espécie devem ser semeadas logo após a sua extração dos frutos.

    PALAVRAS-CHAVE: Frutífera do cerrado; germinação; ácido.

    Araticum seeds (Annona crassiflora Mart. have a slow and uneven pattern of germination, negatively affecting its propagation. The experiment was conducted from March/1999 through November/2001, at the Goiás State Federal University, Agronomy and Food Engineering School, using seeds coming from trees growing in natural environment in the Northeast region of Goiás State. At random, one ripe

  16. Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes Direct organogenesis in nodal explants of mangabeira (Hancornia speciosa Gomes

    Directory of Open Access Journals (Sweden)

    Fernanda Pereira Soares

    2007-08-01

    Full Text Available A mangabeira, espécie nativa do Cerrado, destaca-se por possuir um grande potencial como planta frutífera e produtora de borracha. No entanto, suas sementes apresentam recalcitrância, dificultando sua propagação, o que torna evidente a necessidade da obtenção de mudas por via assexuada. Neste contexto, o cultivo in vitro apresenta-se como uma alternativa a ser utilizada. Com o presente trabalho, visou-se estabelecer uma metodologia de micropropagação da mangabeira via organogênese direta. Para a obtenção de brotações, segmentos caulinares com até duas gemas laterais foram inoculados em meio de cultura WPM, suplementado com diferentes concentrações de BAP (0,0; 1,0; 2,0; 3,0; 4,0 e 5,0 mg L-1. Para o enraizamento, brotações foram inoculadas em meio WPM, suplementado com 0,1% de carvão ativado e diferentes concentrações de ANA (0,0; 1,0; 2,0 e 3,0 mg L-1 ou AIB (0,0; 1,0; 2,0; 3,0 e 4,0 mg L-1. As brotações inoculadas permaneceram em sala de crescimento por 15 dias, quando foram transferidas para meio WPM sem reguladores de crescimento. As concentrações de 5,0 mg L-1 e 3,0 mg L-1 de BAP foram as mais eficientes na indução, respectivamente, de brotações e gemas. O maior comprimento das brotações foi verificado em meio suplementado com 1,0 mg L-1 ou 2,0 mg L-1 de BAP. A maior formação de calos ocorreu em meio suplementado com 4,0 mg L-1 dessa citocinina. A auxina ANA não se mostrou eficiente, nas concentrações testadas, no enraizamento in vitro de brotações de mangabeira. Na presença de 3,0 mg L-1 de AIB, 20% das brotações enraizaram.The mangabeira presents potential for fruit and rubber production. Since the seeds are recalcitrant which makes difficult its propagation, new approaches are needed in order to obtain plants through asexual methods. In this context, the process of in vitro culture presents as an alternative for the mangabeira propagation. The objective of the present work was to establish a

  17. Efeito de diferentes meios nutritivos e fitorreguladores visando à otimização da calogênese de Annona mucosa (Jacq.

    Directory of Open Access Journals (Sweden)

    T.J.S. Barboza

    2014-12-01

    Full Text Available Annona mucosa é uma árvore frutífera da família Annonaceae, produtora de importantes metabólitos secundários de interesse medicinal, como lignanas, acetogeninas e alcaloides. A cultura in vitro de calos representa um importante recurso para a produção contínua de metabólitos, viabilizando a conservação da biodiversidade química e a obtenção controlada de material para estudos biológicos e fitoquímicos. O objetivo deste trabalho foi otimizar a produção de calos friáveis de A. mucosa, avaliando o efeito de diferentes meios nutritivos e fitorreguladores. Segmentos de folha e de hipocótilo de plântulas germinadas in vivo foram utilizados como explantes e inoculados nos meios de cultura MS, WPM e B5 suplementados com picloram (2 - 20µM isolado ou combinado com as citocininas BAP, KIN ou TDZ (0,2 - 1µM. As culturas foram mantidas a 26±2ºC, no escuro, com subcultivos mensais. A produção de calos foi avaliada por aferição do peso dos calos, após 90 dias. Em todos os tratamentos na presença da auxina picloram, o cultivo de hipocótilos resultou em maior porcentagem de formação de calos, sobretudo no meio de cultura WPM. A associação com TDZ produziu massa calogênica friável altamente proliferativa e ausente de oxidação, alcançando valores superiores àqueles obtidos em trabalhos prévios com a espécie. Os resultados viabilizam o uso do material em suspensões celulares e posterior caracterização fitoquímica para a exploração da produção in vitro de metabólitos da espécie.

  18. Propagação in vitro de Limonium latifolium Kuntze (Plumbaginaceae In vitro propagation of Limonium latifolium Kuntze (Plümbaginaceae

    Directory of Open Access Journals (Sweden)

    Claudimar Sidnei Fior

    2000-08-01

    Full Text Available Limonium latifoKum Kuntze é uma flor de corte cuja produção comercial de mudas é viabilizada através do cultivo de tecidos. Com o objetivo de aperfeiçoar o protocolo para a propagação clonal in vitro, fez-se uma sequência de estudos em que foram avaliados: viabilidade do uso de segmentos nadais do eixo da inflorescência imatura como explantes; tipos e concentrações de citocininas (cinetina-KIN e benzilaminopurina-BAP na regeneração; tipos e concentrações de auxinas (ácido naftalenoacético-ANA e ácido indolbutírico-AIB na fase de enraizamento in vitro; e técnicas de aclimatizaçao. Explantes oriundos de segmentos nadais da parte apical do eixo da inflorescência imatura são viáveis para a micropropagaçao de L. latifolium. Na fase de regeneração, os melhores resultados foram obtidos com o uso de meio de cultivo MS com 0,7mg de BAP/l por 35 dias. A fase de enraizamento m vitro foi feita com a manutenção dos explantes por 30 dias em meio MS acrescido de 1mg de AIB/l , garantindo ótimo índice de sobrevivência após a transferência para in vivo. A aclimatiwção foi feita sob cobertura plástica, em bancada de casa de vegetação, à temperatura ambiente e com irrigação por nebulização. Utilizaram-se, como recipientes, bandejas multicelulares de isopor, com 242 células e aproximadamente 10cm³ cada, preenchidas com casca de arroz carbonizada e esterilizada. As mudas receberam, quinzenalmente, adubação líquida com 0,5g/l de um adubo comercial (15:5:l5+micronutrientes. O processo in vitro dura 100 a 120 dias e um explante origina 15 a 30 mudas.Limonium latifolium Kuntze is a cut flower commercialy propagated in vitro. To develop and improve the micropropagation protocol, a sequence of assays was developed to evaluate performance of node explants; effect of cytokinins concentration (kinetin-KlN and 6-benzyl-aminopurin-BA on the regeneration rate; the presence of BA during the multiplication phase; concentration of

  19. Response of root explants to in vitro cultivation of marketable garlic cultivars Resposta dos explantes radiculares ao cultivo in vitro de cultivares comerciais de alho

    Directory of Open Access Journals (Sweden)

    Danielle C Scotton

    2013-03-01

    como garantir a conservação genética das variedades. Nosso objetivo foi analisar a regeneração in vitro de oito cultivares comerciais de alho usando segmentos de raiz como explante. Para cada genótipo, explantes derivados de bulbilho foram isolados e introduzidos em meio MS suplementado com 2,4-D e 2-iP. Calos foram transferidos para meio MS suplementado com 8,8 mM BAP e 0,1 mM NAA (meio de regeneração A, ou com 4,6 mM cinetina somente (meio de regeneração B. Os calos foram avaliados quanto à frequência de regeneração após sessenta dias de cultivo in vitro. A cultivar nobre 'Jonas' apresentou as maiores taxas de regeneração entre as cultivares testadas. O meio de cultura A, o qual continha auxina e citocinina, induziu as maiores taxas de regeneração em todas as cultivares. O processo aqui descrito é simples, reprodutível e pode ser usado potencialmente como uma ferramenta em estratégias de melhoramento para outras cultivares comerciais e genótipos de alho.

  20. Morpho-anatomical characterization of embryogenic calluses from immature zygotic embryo of peach palm during somatic embryogenesis =

    Directory of Open Access Journals (Sweden)

    Simone de Alencar Maciel

    2010-04-01

    Full Text Available The objective of this study was to morpho-anatomically characterizenodular embryogenic calluses from zygotic embryos of peach palm during the induction of somatic embryogenesis. Immature zygotic embryos were pre-treated in MS medium added to Picloram and 2,4-D (25 μM and BAP (0, 5, 10 μM. After three months, primary calluses were transferred to MS induction medium added to Picloram and 2,4-D (450 μM. After six months, the embryogenic calluses were then histologically analyzed and cultivated in the maturation medium. The competent tissues of the zygotic embryos differentiated embryogenic calluses under action of both Picloram and 2,4-D auxins (450 μM, where the presence of multi-granular structures were observed. Histological observations showed that in the nodular embryogenic calluses, the outlying parenchymal cells exhibit cellular characteristics of high mitotic activity. Differentiation of tracheal elements exists in embryogenic calluses connecting the callus to the explant. The evaluated cytokinin/auxin interaction influences the development of embryogenic calluses and globular structures.O objetivo deste trabalho foi caracterizar morfoanatomicamente calos nodulares embriogênicos originados de embriões zigóticos de pupunheira durante a indução da embriogênese somática. Embriões zigóticos imaturos de pupunha foram inicialmente pré-tratados em meio de cultura MS, solidificado com 2,5 g L-1 de phytagel® e suplementado com Picloram e 2,4-D na concentração de 25 μM e BAP (0, 5, 10 μM. Após três meses, os calos primários foram transferidos para meio de indução, com Picloram e 2,4-D (450 μM. Após seis meses, os calosnodulares embriogênicos formados foram então analisados histologicamente e repicados para o meio de maturação para a progressão das estruturas multigranulares embriogênicas. Verificou-seque os tecidos competentes dos embriões zigóticos imaturos diferenciaram nódulos embriogênicos pela ação de ambas

  1. IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea

    Directory of Open Access Journals (Sweden)

    Alexandre Bosco de Oliveira

    2010-05-01

    ="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="en-US" align="JUSTIFY">KEY-WORDS: Disinfection; growth regulator; callus; rooting.

    O trabalho foi realizado visando a desenvolver um protocolo para micropropagação de Mussaenda erytrophylla cv. Rosea. Na assepsia de segmentos de brácteas, utilizou-se hipoclorito de cálcio ou hipoclorito de sódio (2,5%, com ou sem imersão prévia em álcool 70%. Para indução de calos, os segmentos de brácteas foram inoculados em meio MS com diferentes concentrações de BAP e ANA. Para indução de gemas, os calos foram inoculados em meio MS com diferentes concentrações das citocininas BAP, 2iP e CIN (0,0 mg L-1; 0,5 mg L-1; 1,0 mg L-1; e 2,0 mg L-1. Na multiplicação, segmentos nodais de plantas in vitro foram inoculados em meio MS com BAP, CIN e 2iP, nas concentrações de 0,0 mg L-1; 0,5 mg L-1; 1,0 mg L-1; e 2,0 mg L-1. Para o enraizamento, segmentos nodais de plantas in vitro foram inoculados no meio MS e MS/2 da formulação dos sais macronutrientes e diferentes concentrações de AIB (0,00 mg L-1; 0,25 mg L-1; 0,50 mg L-1; e 1,00 mg L-1. O melhor tratamento para a desinfestação foi aquele em que utilizou-se hipoclorito de cálcio associado à imersão em álcool. Para a indução de calos, o meio MS com 0,4 mg L-1 de ANA + 4,0 mg L-1 de BAP mostrou-se o mais efetivo. A

  2. Propagação vegetativa in vitro de cultivares de macieira In vitro propagation of apple cultivars in Brazil

    Directory of Open Access Journals (Sweden)

    Wilson Barbosa

    1986-01-01

    Full Text Available O presente trabalho é resultado de uma série de experimentos desenvolvidos na Seção de Fruticultura de Clima Temperado do IAC, no sentido de adaptar e aperfeiçoar a técnica da micropropagação da macieira. Foram pesquisadas, para os cultivares Rainha e Gala e para as seleções IAC 1381-22, IAC 3881-8 e IAC 4881-11, as principais exigências hormonais, nas várias fases do crescimento e da proliferação dos meristemas, para a indução de raiz, efetivo enraizamento e desenvolvimento apical. Na primeira fase, os meristemas cresceram em meio de cultura constituído da solução salina de Murashige & Skoog com adição de tiamina 1,0 mg/litro, ácido nicotínico, 0,25 mg/litro, inositol 100 mg/litro, ágar 6,5 g/litro, sacarose 30 g/litro é 6-benzilaminopurina (BAP a 0, 2,5, 5,0, 7,5 e 15,0 µM. Na segunda fase, os propágulos foram repicados e colocados em um meio indutor de raiz, com a mesma constituição básica inicial, por cinco dias em ambiente escuro, substituindo-se, porém, a citocinina pela auxina ácido indolbutírico (AIB a 0, 1,0, 2,0, 4,0 e 8,0 µM. Na terceira e última fase, os propágulos foram transferidos em meio para o crescimento das raízes, mantendo-se ainda a mesma constituição básica, reduzindo-se, porém, a sacarose a 10 g/litro e excluindo-se totalmente os fitormônios. Os dados mostraram claramente, para os materiais utilizados, que os melhores índices de crescimento, proliferação e de enraizamento dos explantes, foram obtidos na faixa de 7,5 µM de BAP e 2,0 µM de AIS respectivamente. Os propágulos enraizados, após retirados das condições in vitro, foram plantados em vermiculita expandida, e aclimatizados em miniestufa por quinze dias, sendo finalmente transplantados para vasos com terra e mantidos em casa de vegetação.Experiments on apple micropropagation were carried out in the laboratories of the Instituto Agronômico, Campinas, Brazil. The main hormonal requirements of shoots in the different

  3. BiBAP呼吸机治疗急性左心衰竭的效果评价%The Effect of BiBAP Ventilation in Treatment of Acute Left Heart Failure

    Institute of Scientific and Technical Information of China (English)

    闫小林

    2015-01-01

    Objective To observe BiPAP NIPPV’s clinical therapeutic effect on rescuing left heart failure. Method Randomly divide 82 cases into 2 groups, BiPAP group 83 cases treated with BiPAP respiratory machine for non trauma ventilation therapy on the base of routine anti heart failure medicine, the control one 25 cases were treated with anti heart failure drugs;record the symptoms, signs, hemodynamics, then analyse all parameters. Result In BiPAP group,the clinical symptoms,signs and blood gas were much improved, the marked effective rate 63.8%, the total effective rate 96.3%;79.2%and 85.5%for the control one respectively;two groups had obvious difference. Conclusion Face cover NIPPV treating acute left heart failure can speedily relieve patients’ symptoms and low oxygen blood syndrome, being a quick, safe and effective rescue.%目的:观察BiPAP无创正压通气(NIPPV)在急性左心衰竭抢救中的疗效。方法165例住院急性左心衰竭患者随机分为2组,治疗组(BiPAP组)82例在常规抗心力衰竭药物治疗的基础上时联用BiPAP呼吸机进行无创通气治疗;对照组83例只常规抗心衰药物治疗,记录治疗前后症状、体征、血流动力学。结果 BiPAP组患者临床症状、体征、明显改善,显效率63.8%,总有效率96.3%;对照组显效率79.2%,总有效率85.5%,2组间显效率差异有显著性。结论 BiPAP无创正压通气治疗急性左心衰竭时能迅速改善患者症状和低氧血症,是一种快速安全有效的抢救措施。

  4. THE PRESENT STUDY ON THE TOXIC EFFECT OF BENZO (a) PYRENE (BAP) AND ITS MECHANISM%苯并[a]芘(BaP)的毒性作用与致毒机理研究现状

    Institute of Scientific and Technical Information of China (English)

    刘宇红; 于晓英; 韩宁

    2008-01-01

    本文主要论述了苯并[a]芘(BaP)的毒性作用与致毒机理.并结合目前国内外关于BaP毒性的研究现状,论述了BaP毒性作用的实现及与环境中其他物质或因素的协同作用.

  5. KINETIC STUDY ON TRANSPORT OF BaP IN SOIL-RICE SYSTEM%苯并[a]芘在土壤-水-水稻系统中传输动力学研究

    Institute of Scientific and Technical Information of China (English)

    李书鼎; 张少兰; 马吉春

    1995-01-01

    建立了BaP在土壤水稻系统中传输模型,并对其在系统各组分中的含量变化做了定量分析.实验求得了BaP在系统各相间传输速度常数和分配系数.揭示了水中BaP向水稻体传输路径和它们的速度大小.水稻从水中吸收BaP的速度比其从土壤中吸收的速度高4倍,水中BaP向土壤沉积速度比水稻从水中吸收BaP的速度高7倍.水中BaP在迁移到达水稻体之前,绝大部分被土壤牢牢吸附而很难被水稻吸收.

  6. 焦化废水中致癌污染物BaP的分布及减排控制%Distributing and controlling of carcinogen Bap in the coking waste water

    Institute of Scientific and Technical Information of China (English)

    李莉

    2008-01-01

    从焦化废水中致癌污染物BaP的危害入手,分析其在焦化生产工艺废水中的分布特点,结合目前焦化企业BaP的处理工艺效果和存在问题,提出对现有和新建企业不同时段、不同废水利用途径应分别采取的减排控制方案.

  7. DETERMINATION OF NITRITE AND BAP IN BAKED OR FRIED FOODS FROM A UNIVERSITY AND AROUND%某大学及周边烤炸食品中亚硝酸盐和苯并芘监测

    Institute of Scientific and Technical Information of China (English)

    张巧英; 张龙杰; 唐晓渝; 刘明芳; 刘敏华

    2010-01-01

    [目的]了解某大学及周边摊点烧烤和油炸食品中亚硝酸盐和苯并芘(Benzo(a)pyrene,BaP)的含量.[方法]采集西南大学食堂及周边8个点共26个烧烤和油炸食品,由西南大学食品科学学院测定其亚硝酸盐和BaP的含量. [结果]摊点4的烤羊肉串的亚硝酸盐35.585 mg/kg,超过国家规定的30 ms/ks;食堂3的烤豆干样本中BaP的含量为5.08 μg/kg,食堂4的炸土豆中BaP的含量为5.98 μs/kg,摊点4的全部样本中BaP的含量超过国家规定的5μg/kg水平. [结论]烧烤和油炸食品中存在亚硝酸盐和BaP超标情况,摊点超标情况高于食堂,BaP高于亚硝酸盐,建议尽量避免食用烤炸食品.

  8. Plankton, temperature and other measurements found in dataset OSD taken from the BONDY, BAP EXPLORADOR and other platforms in the Coastal S Pacific, South Pacific and other locations from 1961 to 1965 (NODC Accession 0001140)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical, nutrients, and plankton data were collected using plankton net and bottle casts in the South Pacific Ocean from 01 August 1961 to 09 September 1965. Data...

  9. Anaerobic digestion of solid waste in RAS: Effect of reactor type on the biochemical acidogenic potential (BAP) and assessment of the biochemical methane potential (BMP) by a batch assay

    DEFF Research Database (Denmark)

    Suhr, Karin Isabel; Letelier-Gordo, Carlos Octavio; Lund, Ivar

    2015-01-01

    Anaerobic digestion is a way to utilize the potential energy contained in solid waste produced in recirculating aquaculture systems (RASs), either by providing acidogenic products for driving heterotrophic denitrification on site or by directly producing combustive methane. In this study the bioc...

  10. Cuidado para el bienestar de mujeres gestantes orientado a la detección Precoz de la hemorragia Post parto

    OpenAIRE

    Ladino Santos, Maritza; Martínez Castro, Sandra Milena

    2014-01-01

    La Hemorragia Post Parto (HPP) continua siendo según las cifras una de las causas de muerte materna más significativas a nivel mundial. Aunque se han tomado medidas para su reducción, su prevalencia es alta, lo que motiva a la comunidad científica a trabajar en la búsqueda de estrategias encaminadas a su detección oportuna. La HPP afecta seriamente el proyecto de vida de la paciente, retrasa el vínculo madre e hijo, afecta la estabilidad familiar, su salud física y emo...

  11. Aprendizaje espacial en laberintos en una tarea de discriminación. Los fenómenos de la inhibición latente y del aprendizaje perceptivo.

    OpenAIRE

    Sansa i Aguilar, Joan

    1995-01-01

    [spa] La exposición previa a un estimulo que se usará como estímulo condicionado (EC) en un paradigma de condicionamiento clásico típicamente retrasa el condicionamiento posterior. Este es el fenómeno de la inhibición latente (IL) (Lubow, 1989). Sin embargo, en ocasiones esta exposición tiene el efecto opuesto. Así, en el aprendizaje latente, la exposición a un laberinto de múltiples unidades facilitará la elección del camino correcto cuando posteriormente se introduce e! reforzador (Tolman y...

  12. Crecimiento con igualdad

    OpenAIRE

    Diana Alarcón González

    2000-01-01

    El presente trabajo trata primeramente los temas de estabilidad y crecimiento y la hipótesis de Kuznets. A continuación, trata los temas de la desigualdad y crecimiento en América Latina así como la hipótesis que explicarían una relación positiva entre crecimiento e igualdad. Seguidamente, se argumenta como la desigualdad retrasa el crecimiento, para por último, formular algunas conclusiones sobre estos temas y el desarrollo latinoamericano.

  13. Efectos de los antioxidantes y la fagocitosis bacteriana sobre la apoptosis de los neutrófilos

    OpenAIRE

    Bonilla del Río, Zuriñe

    2013-01-01

    La fagocitosis bacteriana por los neutrófilos retrasa la apoptosis de los mismos cuando la relación bacteria/neutrófilo es baja, y la acelera cuando dicha relación es alta. Las bacterias también inducen en los neutrófilos la producción de sustancias reactivas de oxígeno que incrementarían su apoptosis, y de citoquinas que podrían retrasarla. El antioxidante DPI (difenileniodonio), inhibidor específico de la NADPH oxidasa de los neutrófilos, podría modificar la apoptosis de...

  14. De la evaluación a la intervención: atención conjunta, directividad y desarrollo del lenguaje en niños con síndrome de Down

    OpenAIRE

    Galeote, Miguel; Checa, Elena; Serrano, Antonio; Rey, Rocío

    2004-01-01

    Se acepta ampliamente como algo demostrado que el exceso de directividad (acción influyente y dirigida) retrasa el desarrollo del lenguaje. En consecuencia, muchos programas de intervención incluyen como recomendación que los padres no muestren conductas directivas con sus hijos. Un caso particular lo constituyen los padres de niños con síndrome de Down, quienes suelen mostrar una alta directividad. En un análisis más detenido, no obstante, es preciso matizar dicha recomendación. Considerar,...

  15. 75 FR 23319 - Notice of Funding Availability for the Small Business Transportation Resource Center Program

    Science.gov (United States)

    2010-05-03

    ... BAP and how the Reimbursable Fee Program works. A minimum of 10 DBE firms per workshop should... Program (DBE ARRA BAP) applicants to increase the probability of reimbursement approval and generate a minimum of 5 approved DBE ARRA BAP applications until September 8, 2010 or until notice of cessation...

  16. The Broader Autism Phenotype and Friendships in Non-Clinical Dyads

    Science.gov (United States)

    Wainer, Allison L.; Block, Nicole; Donnellan, M. Brent; Ingersoll, Brooke

    2013-01-01

    The broader autism phenotype (BAP) is a set of subclinical traits qualitatively similar to those observed in autism spectrum disorders. The current study sought to elucidate the association between self- and informant-reports of the BAP and friendships, in a non-clinical sample of college student dyads. Self-informant agreement of the BAP and…

  17. 75 FR 42181 - Notice of Funding Availability for the Small Business Transportation Resource Center Program

    Science.gov (United States)

    2010-07-20

    ... DBEs on the DOT ARRA BAP and how the Reimbursable Fee Program works. A minimum of 10 DBE firms per... Assistance Reimbursable Fee Program (DBE ARRA BAP) applicants to increase the probability of reimbursement approval and generate a minimum of 5 approved DBE ARRA BAP applications until September 8, 2010 or...

  18. Functional differentiation of SWI/SNF remodelers in transcription and cell cycle control

    NARCIS (Netherlands)

    Y.M. Moshkin (Yuri); L. Mohrmann (Lisette); W.F.J. van IJcken (Wilfred); C.P. Verrijzer (Peter)

    2007-01-01

    textabstractDrosophila BAP and PBAP represent two evolutionarily conserved subclasses of SWI/SNF chromatin remodelers. The two complexes share the same core subunits, including the BRM ATPase, but differ in a few signature subunits: OSA defines BAP, whereas Polybromo (PB) and BAP170 specify PBAP. He

  19. CHARACTERIZATION OF STABLE BENZOLALPYRENE-7,8-QUINONE-DNA ADDUCTS IN CALF THYMUS DNA AND POLYDEOXYNUCLEOTIDES

    Science.gov (United States)

    Bcnzo[a]pyrene-7,8-dione (BPQ) is a reactive aldo-keto reductase-mediated product of B[a]P-7,8-diol, a major P450/epoxide hydrolase metabolite of the multi-species carcinogen, B[a]P. The role of BPQ in B[a]P's genotoxicity and carcinogenesis is evolving. Toxicity pathways involvi...

  20. CHARACTERIZATION OF STABLE BENZO(A)PYRENE-7,8-QUINONE-DNA ADDUCTS IN CALF THYMUS DNA

    Science.gov (United States)

    Benzo[alpyrene-7,8-dione (BPQ) is a reactive aldo-keto reductase-mediated product of B[a]P-7,8-diol, a major P450/epoxide hydrolase metabolite of the multi-species carcinogen, B[a]P. The role of BPQ in B[a]P's genotoxicity and carcinogenesis is evolving. Toxicity pathways involvi...

  1. Micropropagação e conservação de Macrosyphonia velame (St. Hil. Muell. Arg. em banco de germoplasma in vitro Micropropagation and conservation of Macrosyphonia velame (St. Hil. Muell. Arg. in vitro germoplasm bank

    Directory of Open Access Journals (Sweden)

    Letícia Maria Martins

    2011-03-01

    Full Text Available A Macrosyphonia velame é uma planta medicinal do Cerrado, pertencente à família Apocynaceae, e conhecida popularmente como velame branco. Extratos de raízes de velame são utilizados pela população como depurativo e anti-sifilítico. Este trabalho teve como objetivo desenvolver o protocolo de micropropagação de M. velame, com vistas à conservação da espécie em Banco do Germoplasma in vitro. Sementes foram coletadas nos municípios de Sacramento, Tapira e Araxá, MG, e utilizadas como fonte de explantes. Segmentos nodais de plântulas axênicas foram transferidos para meio MS/2 suplementado com BAP, Cinetina, 2ip e TDZ em diferentes concentrações (0,25; 5,0 e 1,0mg L-1. Para o enraizamento, brotações com 2cm de altura foram transferidas para meios MS/2 suplementado com 1,0; 5,0; e 10,0mg L-1 de IBA ou ANA e cultivadas por 5, 10 e 30 dias, sendo em seguida subcultivadas em MS/2 por mais trinta dias e só então avaliadas quanto à formação de raízes. Na aclimatização e enraizamento ex vitro, foram utilizados substratos Plantmax®, areia e solo individualmente ou em combinações (1:1 de areia/Plantmax®; areia/solo; Plantmax®/solo. Para o estabelecimento do Banco de Germoplasma, brotações com 3,5cm foram transferidas para meio MS/2 suplementado com 2% de sacarose + 4% de manitol ou sorbitol; 2% de sacarose + 4% de manitol ou sorbitol + 2mg L-1 de pantotenato de cálcio; 2% de sacarose + 4% de manitol ou sorbitol + 2mg L-1 de espermidina. A porcentagem de germinação in vitro foi baixa, 33%, 4% e 2% das sementes coletadas em Araxá, Tapira e Sacramento, respectivamente. O meio MS/2 sem adição de citocinina promoveu a proliferação de brotos (4,0 por gema, elongação (5,2cm, número de gemas (8,6 e reduziu vitrificação (4%. Em relação aos substratos testados, as plântulas se desenvolveram melhor no Plantmax®, sendo que 40% das plântulas sobreviveram e a maioria apresentou formação de raízes. Pl

  2. Predicting lung dosimetry of inhaled particleborne benzo[a]pyrene using physiologically based pharmacokinetic modeling

    Science.gov (United States)

    Campbell, Jerry; Franzen, Allison; Van Landingham, Cynthia; Lumpkin, Michael; Crowell, Susan; Meredith, Clive; Loccisano, Anne; Gentry, Robinan; Clewell, Harvey

    2016-01-01

    Abstract Benzo[a]pyrene (BaP) is a by-product of incomplete combustion of fossil fuels and plant/wood products, including tobacco. A physiologically based pharmacokinetic (PBPK) model for BaP for the rat was extended to simulate inhalation exposures to BaP in rats and humans including particle deposition and dissolution of absorbed BaP and renal elimination of 3-hydroxy benzo[a]pyrene (3-OH BaP) in humans. The clearance of particle-associated BaP from lung based on existing data in rats and dogs suggest that the process is bi-phasic. An initial rapid clearance was represented by BaP released from particles followed by a slower first-order clearance that follows particle kinetics. Parameter values for BaP-particle dissociation were estimated using inhalation data from isolated/ventilated/perfused rat lungs and optimized in the extended inhalation model using available rat data. Simulations of acute inhalation exposures in rats identified specific data needs including systemic elimination of BaP metabolites, diffusion-limited transfer rates of BaP from lung tissue to blood and the quantitative role of macrophage-mediated and ciliated clearance mechanisms. The updated BaP model provides very good prediction of the urinary 3-OH BaP concentrations and the relative difference between measured 3-OH BaP in nonsmokers versus smokers. This PBPK model for inhaled BaP is a preliminary tool for quantifying lung BaP dosimetry in rat and humans and was used to prioritize data needs that would provide significant model refinement and robust internal dosimetry capabilities. PMID:27569524

  3. Mitochondrial dysfunction and transactivation of p53-dependent apoptotic genes in BaP-treated human fetal lung fibroblasts.

    Science.gov (United States)

    Yang, Guangtao; Jiang, Ying; Rao, Kaimin; Chen, Xi; Wang, Qian; Liu, Ailin; Xiong, Wei; Yuan, Jing

    2011-12-01

    Benzo(a)pyrene (BaP) has been shown to be an inducer of apoptosis. However, mechanisms involved in BaP-induced mitochondrial dysfunction are not well-known. In this study, human fetal lung fibroblasts cells were treated with BaP (8, 16, 32, 64 and 128 μM) for 4 and 12 h. Cell viability, intracellular level of reactive oxygen species (ROS), total antioxidant capacity (T-AOC), mitochondrial membrane potential (ΔΨ(m)) and cytochrome c release were determined. Changes in transcriptional levels of p53-dependent apoptotic genes (p53, APAF1, CASPASE3, CASPASE9, NOXA and PUMA) were measured. At time point of 4 h, BaP induced the intracellular ROS generation in 64 (p BaP groups (p BaP groups (p BaP groups (p BaP group (p BaP groups (p BaP group a relatively little expression of p53 mRNA was observed (p BaP promoted the generation of excessive ROS and subsequently the mitochondrial depolarization, whereas transactivations of the p53-dependent apoptotic genes were significantly induced at the later period.

  4. Functional analysis of the two Brassica AP3 genes involved in apetalous and stamen carpelloid phenotypes.

    Directory of Open Access Journals (Sweden)

    Yanfeng Zhang

    Full Text Available The Arabidopsis homeotic genes APETALA3 (AP3 and PISTILLATA (PI are B genes which encode MADS-box transcription factors and specify petal and stamen identities. In the current study, the stamen carpelloid (SC mutants, HGMS and AMS, of B. rapa and B. napus were investigated and two types of AP3 genes, B.AP3.a and B.AP3.b, were functional characterized. B.AP3.a and B.AP3.b share high similarity in amino acid sequences except for 8 residues difference located at the C-terminus. Loss of this 8 residues in B.AP3.b led to the change of PI-derived motifs. Meanwhile, B.AP3.a specified petal and stamen development, whereas B.AP3.b only specified stamen development. In B. rapa, the mutations of both genes generated the SC mutant HGMS. In B. napus that contained two B.AP3.a and two B.AP3.b, loss of the two B.AP3.a functions was the key reason for the apetalous mutation, however, the loss-of-function in all four AP3 was related to the SC mutant AMS. We inferred that the 8 residues or the PI-derived motif in AP3 gene probably relates to petal formation.

  5. 苯并[a]芘和镉暴露对食蚊鱼求偶行为的影响%Effects on the courtship behavior of mosquitofish(Gambusia affinis) exposed to 3,4-benzopyrene (BaP) and cadmium (Cd2+)

    Institute of Scientific and Technical Information of China (English)

    苏方; 谢勇平; 方展强

    2013-01-01

    研究苯并[a]芘(BaP)和镉(Cd2+)暴露对雄性食蚊鱼(Gambusia affinis)求偶行为的影响.设对照组和实验组,BaP暴露浓度为0、0.1、100 μg/L;Cd2+暴露浓度为0、5、500 nmol/L;分别暴露6和8周.暴露实验结束后,观察雄鱼与雌鱼配对(1∶1)的求偶行为,并作录像记录分析.结果显示,雄鱼在0.1和100 μg/L的BaP分别暴露6周后其对雌鱼的求偶行为开始明显降低,并随着暴露浓度的升高和时间的延长而呈现下降趋势;BaP高浓度组(100 μg/L)暴露8周后对雄鱼有严重的致死效应.暴露在5和500 nmol/L的Cd2+至8周后雄鱼求偶行为明显降低;雌鱼暴露在低浓度BaP和Cd2+中其对雄鱼的求偶行为无明显影响(P>0.05);但分别在高浓度100 μg/L BaP和500 nmol/L Cd2+中暴露后,雄鱼对雌鱼的求偶行为显著减少.结果表明,BaP和Cd2+暴露均可降低雄性食蚊鱼的求偶行为.

  6. 纳米TiO2催化下表层土壤中BaP的紫外光降解%Photodegradation of BaP on Soil Surfaces in the Presence of Nano-TiO2 Under UV-light Irradiation

    Institute of Scientific and Technical Information of China (English)

    张利红; 李培军; 李雪梅; 陈忠林

    2009-01-01

    采用光解试验,研究了紫外照射与纳米TiO2联合作用下,土壤表层中苯并[a]芘(BaP)的降解动力学;同时考察了催化剂的浓度、土壤pH、腐植酸和光质对BaP的光催化降解的影响.结果表明,土壤中BaP的光催化降解表现为准一级动力学.催化剂TiO2可以明显地促进土壤中BaP的光降解,较少量的催化剂(0.5%)使光解的半衰期从363.22 h减少到103.26 h.H+和OH-离子对BaP的催化光解起促进作用,在酸性和碱性土壤中BaP光催化降解高于中性土壤,酸性土壤中的降解速率最快.腐殖质吸收紫外光照射时,产生的活性氧中间产物能够攻击BaP,添加腐植酸能增加土壤表层中BaP的光催化降解.BaP的光解半衰期从无外加腐植酸的89.34 h,减少到添加浓度分别为5、10、20和40 mg·kg-1的29.37、32.69、35.73和38.51 h.BaP的催化降解随波长的增加而降低,在波长254、310和365 nm下,BaP降解的一级动力学常数分别为0.007 8 h-1、0.006 1 h-1和0.005 h-1.

  7. 骨碱性磷酸酶与碱性磷酸酶在佝偻病诊断中的比较%Bone alkaline phosphatase versus serum alkaline phosphatase in the diagnosis of rickets

    Institute of Scientific and Technical Information of China (English)

    林岚; 陈力; 张春玲; 杨延萍; 肖玉联

    2011-01-01

    Objective To explore the significance of quantitative and qualitative measurements of bone alkaline phosphatase ( BAP-E and BAP-CIT ) and detection of serum AP in the diagnosis of rickets. Methods Serum AP level was measured, quantitative BAP was detected by ELISA, and qualitative BAP was determined by CIT in 55 children with rickets and 26 control subjects. Results AP level and BAP-E were ( 164.38 ± 23.87 ) U/L and ( 57.99 ± 7.67 ) U/L in controls, and were ( 209.50 ± 44.28 ) U/L, ( 80.38 ± 15.75 ) U/L, ( 206.06 ± 30.80 ) U/L, ( 78.05± 11.69) U/L, ( 1000.47 ± 464.65) U/L, and (283.22 ± 96.42) U/L in mild or severe rickets ,respectively. AP and BAP-E were elevated significantly in children with rickets ( P< 0.05 ), and there were significant differences between the severe groups and the mild groups ( P < 0.05 ). AP positively related with BAP-E ( r= 0.921, P< 0.01 ) and BAP-CIT ( r= 0.49, P< 0.01 ). In the control group,BAP-CIT within the normal range was only in one patient and that beyond the normal range was in the remaining patients. Conclusions AP and BAP-E are positively correlated with the severity of rickets.AP may be used in severe patients. BAP-CIT should be used carefully in the diagnosis of rickets.%目的 了解定量骨碱性磷酸酶( BAP-E)、定性骨碱性磷酸酶(BAP-CIT)、血清碱性磷酸酶(AP)三个指标在佝偻病诊断中的意义。方法 检测55例佝偻病患儿、26例对照婴幼儿血清AP、BAP-E、BAP-CIT,BAP-E采用ELISA法,BAP-CIT用全血干化学和免疫浓缩技术。结果 佝偻病患儿的AP和BAP-E较对照组(164.38 U/L±23.87 U/L,57.99 U/L±7.67 U/L)明显增高(P<0.05),中、重度组佝偻病患儿的AP和BAP-E( 1000.47 U/L±464.65U/L,283.22 U/L±96.42 U/L)较轻度组(209.50 U/L±44.28 U/L,80.38 U/L±15.75 U/L;206.06 U/L±30.80 U/L,78.05 U/L±11.69 U/L)明显增高(P<0.05),对照组BAP-CIT仅1例在试剂盒的正常范围≤200 U/L,余皆在异常范围(200-300) U/L,AP

  8. 6-Benzylaminopurine inhibits growth of Monilinia fructicola and induces defense-related mechanism in peach fruit.

    Science.gov (United States)

    Zhang, Yangyang; Zeng, Lizhen; Yang, Jiali; Zheng, Xiaodong; Yu, Ting

    2015-11-15

    This study demonstrated the inhibitory effect of 6-benzylaminopurine (BAP), the first generation synthetic cytokinin, on the invasion of Monilinia fructicola in peach fruit and the possible mechanism involved for the first time. Our results suggested that BAP treatment had a 63% lower disease incidence and approximately 10 times lower lesion diameter compared to the control throughout the incubation period. In vitro BAP showed a direct inhibitory effect on M. fructicola spore germination. BAP could prevent fruit texture deterioration and protect the cell membrane from oxidative stress, while no adverse effects were observed on fruit quality maintenance. Analysis of defense-related enzymes activities indicated that the use of BAP induced higher specific polyphenol oxidase and peroxidase activities which triggered stronger host defensive responses. Thus, our results verified the proposed mechanism of BAP in controlling M. fructicola by direct inhibitory effect, delay peach senescence and activation of defensive enzymes.

  9. Titanium dioxide nanoparticles as guardian against environmental carcinogen benzo[alpha]pyrene.

    Directory of Open Access Journals (Sweden)

    Anupam Dhasmana

    Full Text Available Polycyclic aromatic hydrocarbons (PAH, like Benzo[alpha]Pyrene (BaP are known to cause a number of toxic manifestations including lung cancer. As Titanium dioxide Nanoparticles (TiO2 NPs have recently been shown to adsorb a number of PAHs from soil and water, we investigated whether TiO2 NPs could provide protection against the BaP induced toxicity in biological system. A549 cells when co-exposed with BaP (25 µM, 50 µM and 75 µM along with 0.1 µg/ml,0.5 µg/ml and 1 µg/ml of TiO2 NPs, showed significant reduction in the toxic effects of BaP, as measured by Micronucleus Assay, MTT Assay and ROS Assay. In order to explore the mechanism of protection by TiO2 NP against BaP, we performed in silico studies. BaP and other PAHs are known to enter the cell via aromatic hydrocarbon receptor (AHR. TiO2 NP showed a much higher docking score with AHR (12074 as compared to the docking score of BaP with AHR (4600. This indicates a preferential binding of TiO2 NP with the AHR, in case if both the TiO2 NP and BaP are present. Further, we have done the docking of BaP with the TiO2 NP bound AHR-complex (score 4710, and observed that BaP showed strong adsorption on TiO2 NP itself, and not at its original binding site (at AHR. TiO2 NPs thereby prevent the entry of BaP in to the cell via AHR and hence protect cells against the deleterious effects induced by BaP.

  10. The broad autism phenotype predicts child functioning in autism spectrum disorders

    OpenAIRE

    Maxwell, Christina R.; Parish-Morris, Julia; Hsin, Olivia; Bush, Jennifer C; Schultz, Robert T.

    2013-01-01

    Background Broad autism phenotype (BAP) is a milder expression of the social and communication impairments seen in autism spectrum disorders (ASD). While prior studies characterized the BAP in unaffected family members of probands with ASD, the relationship between parental BAP traits and proband symptomatology remains poorly understood. This study utilizes the Broad Autism Phenotype Questionnaire (BAPQ) in parents and the Social Responsiveness Scale (SRS) in children to examine this connecti...

  11. Effect of plant growth regulators on fatty acids composition in Jatropha curcas L. callus culture.

    Science.gov (United States)

    Hernandez, Ludwi Rodríguez; Mendiola, Martha A Rodríguez; Castro, Carlos Arias; Gutiérrez-Miceli, Federico A

    2015-01-01

    The influence of Naphtaleneacetic acid (NAA) and 6-Benzylaminopurine (BAP) on callus formation, its morphology and fatty acids profile were examined from Jatropha curcas L. Embryo from seeds of J. curcas L. were sown in Murashige and skoog (MS) medium with NAA and BAP. All treatments induced callus formation, however callus morphology was different in most of the treatments. Higher callus biomass was presented with 1.0 NAA + 0.5 BAP mg/L. Plant growth regulators modifies the fatty acids profile in callus of J. curcas L. BAP was induced linoleic and linolenic acids. PMID:25757437

  12. The application of the comet assay to assess the genotoxicity of environmental pollutants in the nematode Caenorhabditis elegans

    OpenAIRE

    Imanikia, Soudabeh; Galea, Francesca; Nagy, Eszter; Phillips, David H.; Stürzenbaum, Stephen R; Arlt, Volker M.

    2016-01-01

    This study aimed to establish a protocol for cell dissociation from the nematode Caenorhabditis elegans (C. elegans) to assess the genotoxicity of the environmental pollutant benzo[a]pyrene (BaP) using the alkaline version of the single cell electrophoresis assay (comet assay). BaP genotoxicity was assessed in C. elegans (wild-type [WT]; N2, Bristol) after 48 h exposure (0 to 40 μM). Induction of comets by BaP was concentration-dependent up to 20 μM; comet % tail DNA was ∼30% at 20 μM BaP and...

  13. Effect of plant growth regulators on fatty acids composition in Jatropha curcas L. callus culture.

    Science.gov (United States)

    Hernandez, Ludwi Rodríguez; Mendiola, Martha A Rodríguez; Castro, Carlos Arias; Gutiérrez-Miceli, Federico A

    2015-01-01

    The influence of Naphtaleneacetic acid (NAA) and 6-Benzylaminopurine (BAP) on callus formation, its morphology and fatty acids profile were examined from Jatropha curcas L. Embryo from seeds of J. curcas L. were sown in Murashige and skoog (MS) medium with NAA and BAP. All treatments induced callus formation, however callus morphology was different in most of the treatments. Higher callus biomass was presented with 1.0 NAA + 0.5 BAP mg/L. Plant growth regulators modifies the fatty acids profile in callus of J. curcas L. BAP was induced linoleic and linolenic acids.

  14. Risk of Air Pollution in Relation to Cancer in the Nordic Countries

    DEFF Research Database (Denmark)

    Fauser, Patrik; Ketzel, Matthias; Becker, Thomas;

    no exceedances of the EU air quality values. The only exceedance of US-EPA 1:100,000 cancer risk concentrations occurs for the PAH BaP in Denmark. However, the EU target value threshold for BaP is not exceeded. No emission data for BaP are available for the other countries and important uncertainties are still...... related to the Danish emissions. Long-range transport is significant except for BaP that originates mostly from residential wood combustion. It is recommended to monitor the influence from residential wood combustion more extensively, and to analyze longer time trends for long-term human exposure....

  15. Functional Analysis of the Two Brassica AP3 Genes Involved in Apetalous and Stamen Carpelloid Phenotypes

    OpenAIRE

    Zhang, Yanfeng; Wang, Xuefang; Zhang, Wenxue; Yu, Fei; Tian, Jianhua; Li, Dianrong; Guo, Aiguang

    2011-01-01

    The Arabidopsis homeotic genes APETALA3 (AP3) and PISTILLATA (PI) are B genes which encode MADS-box transcription factors and specify petal and stamen identities. In the current study, the stamen carpelloid (SC) mutants, HGMS and AMS, of B. rapa and B. napus were investigated and two types of AP3 genes, B.AP3.a and B.AP3.b, were functional characterized. B.AP3.a and B.AP3.b share high similarity in amino acid sequences except for 8 residues difference located at the C-terminus. Loss of this 8...

  16. Estudo comparativo da produção de metabólitos secundários em cultura de células e na planta in natura de Gomphrena globosa (Amaranthaceae

    Directory of Open Access Journals (Sweden)

    A.C.G.M André

    2003-01-01

    Full Text Available A cultura de calos de Gomphrena globosa a partir de segmentos foliares foi estabelecida em meio contendo combinações dos fitoreguladores auxina/citocinina. Metabólitos secundários identificados através da fitoquímica da planta in natura, foram utilizados como padrões em análises por cromatografia líquida de alta eficiência e cromatografia de gás de extratos da cultura. O estudo comparativo revelou que os calos foram capazes de produzir alguns metabólitos sintetizados pela planta in natura.

  17. Criptococosis cutánea primaria en paciente inmunocompetente.

    Science.gov (United States)

    Osorio, Igor Vázquez; García-Rodiño, Sara; Rodríguez-Rodríguez, Marta; Labandeira, Javier; Suárez-Peñaranda, José Manuel; Sánchez-Aguilar, MDolores; Vázquez-Veiga, Hugo

    2016-01-01

    La criptococosis cutánea es una micosis propia de pacientes inmunodeprimidos, sobre todo aquellos con infección por el virusde la inmunodeficiencia humana (VIH). Sin embargo, existen casos infrecuentes de criptococosis cutánea en pacientes inmunocompetentes, que suelen simular otras dermatosis, lo que retrasa su diagnóstico y tratamiento. Presentamos el caso de un varón pluripatológico de 79 años, con úlceras dolorosas en dorso de mano derecha que no respondían a tratamientos tópicos. A través del estudio histopatológico y micológico se alcanzó el diagnóstico de criptococosis cutánea primaria, lográndose la remisión de las lesiones tras 6 meses de tratamiento con fluconazol. PMID:27617525

  18. Estimación de incidencia de daños provocados por larvas de Diatraea saccharalis (Lepidoptera: Crambidae) en tallos de maíz mediante evaluación de signos externos de infestación Estimating the incidence of corn stem damage produced by Diatraea saccharalis (Lepidoptera: Crambidae) larva through assessment of external infestation signs

    OpenAIRE

    Serra, G.; E. Trumper

    2006-01-01

    Diatraea saccharalis es la plaga del cultivo de maíz de mayor impacto económico en la región pampeana de la Argentina. Las larvas ocasionan pérdidas por daños de tipo fisiológico y mecánico. Las pérdidas por daño mecánico aumentan a medida que se retrasa la fecha de cosecha, y una de las medidas culturales para prevenirlas es la cosecha anticipada. El objetivo de este trabajo fue estudiar la relación entre el número de entrenudos con galerías producidas por larvas grandes y el número de orifi...

  19. Criptococosis cutánea primaria en paciente inmunocompetente.

    Science.gov (United States)

    Vázquez-Osorio, Igor; García-Rodiño, Sara; Rodríguez-Rodríguez, Marta; Labandeira, Javier; Suárez-Peñaranda, José Manuel; Sánchez-Aguilar, MDolores; Vázquez-Veiga, Hugo

    2016-05-15

    La criptococosis cutánea es una micosis propia de pacientes inmunodeprimidos, sobre todo aquellos con infección por el virusde la inmunodeficiencia humana (VIH). Sin embargo, existen casos infrecuentes de criptococosis cutánea en pacientes inmunocompetentes, que suelen simular otras dermatosis, lo que retrasa su diagnóstico y tratamiento. Presentamos el caso de un varón pluripatológico de 79 años, con úlceras dolorosas en dorso de mano derecha que no respondían a tratamientos tópicos. A través del estudio histopatológico y micológico se alcanzó el diagnóstico de criptococosis cutánea primaria, lográndose la remisión de las lesiones tras 6 meses de tratamiento con fluconazol.

  20. Variación en el contenido de L-dopa y compuestos fenólicos durante el desarrollo de semilla de Vicia faba L.

    OpenAIRE

    García Sánchez, Isabel

    2012-01-01

    Estudios recientes han demostrado que el haba además de su alto contenido de proteína, posee otras sustancias benéficas para la salud, como son los compuestos fenólicos que actúan como antioxidantes y la L-dopa, aminoácido que retrasa los síntomas severos del síndrome de Parkinson. Por lo que el presente trabajo tuvo como objetivo caracterizar la variación de componentes químicos como proteína soluble, compuestos fenólicos, L-dopa y L-tirosina de colectas de Vicia faba L. provenientes de la...

  1. Effects of light on the cytotoxicity and genotoxicity of benzo(a)pyrene and an oil refinery effluent in the newt

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez, M.; l`Haridon, J. [Universite Paul-Sabatier, Toulouse (France)

    1994-12-31

    The genotoxicity and/or toxicity of benzo(a)pyrene (BaP) were evaluated under different lighting conditions in larvae and embryos of the newt Pleurodeles waltl. Visible light alone, UVA alone, or BaP alone had no toxic effects on the larvae. Conversely, toxic effects were observed in animals exposed to BaP + daylight, or BaP + UVA. The genotoxicity of BaP (50 ppb) was halved by its previous exposure to UVA, and was abolished at the lowest concentration (12.5 ppb). In other experiments, the larvae were exposed alternatively to BaP or Irr BaP (18 hours in dark) and UVA (6 hr in water), every day for 8 days. All animals that had accumulated non-irradiated BaP (50 ppb) showed signs of severe toxicity, and 90% died before the end of the test. On the other hand, irradiated BaP (50 ppb) was a 4-fold less toxic and half as genotoxic as non-irradiated BaP. In addition, exposure of the animals to UVA alone for 4 days prior to treatment with BaP did not affect the genotoxicity or toxicity of this hydrocarbon. In the dark, the embryotoxicity of BaP was markedly attenuated by the presence of the jelly coats. Although UVA alone did not affect growth of the embryos, the toxicity of BaP was enhanced by the combined action of the two agents together or in succession (BaP + UVA or BaP then UVA). Larvae were treated with an oil refinery effluent (EF). At 125 ml/l, EF was not found to be genotoxic in the dark. However, in animals exposed to both EF and UVA, there was a progressive increase in level of micronucleated erythrocytes with increasing duration of daily exposure to UVA. Moreover, the genotoxic potential of irradiated EF + UVA was systematically below that of non-irradiated EF + UVA for all durations of exposure to ultraviolet light. Irradiation of this type of effluent might help reduce its harmful effects on aquatic species. Our results also suggest that metabolic activation is not necessary for hydrocarbons to induce toxic effects. 51 refs., 5 tabs., 3 figs.

  2. Modulation of benzo[a]pyrene induced neurotoxicity in female mice actively immunized with a B[a]P–diphtheria toxoid conjugate

    Energy Technology Data Exchange (ETDEWEB)

    Schellenberger, Mario T.; Grova, Nathalie; Farinelle, Sophie; Willième, Stéphanie [Institute of Immunology, Centre de Recherche Public de la Santé/Laboratoire National de Santé, 20A rue Auguste Lumière, L-1950 Luxembourg, Grand-Duchy of Luxembourg (Luxembourg); Schroeder, Henri [University of Nancy, URAFPA, INRA UC340, F-54500 Vandoeuvre-lès-Nancy (France); Muller, Claude P., E-mail: claude.muller@crp-sante.lu [Institute of Immunology, Centre de Recherche Public de la Santé/Laboratoire National de Santé, 20A rue Auguste Lumière, L-1950 Luxembourg, Grand-Duchy of Luxembourg (Luxembourg)

    2013-09-01

    Benzo[a]pyrene (B[a]P) is a small molecular weight carcinogen and the prototype of polycyclic aromatic hydrocarbons (PAHs). While these compounds are primarily known for their carcinogenicity, B[a]P and its metabolites are also neurotoxic for mammalian species. To develop a prophylactic immune strategy against detrimental effects of B[a]P, female Balb/c mice immunized with a B[a]P–diphtheria toxoid (B[a]P–DT) conjugate vaccine were sub-acutely exposed to 2 mg/kg B[a]P and behavioral performances were monitored in tests related to learning and memory, anxiety and motor coordination. mRNA expression of the NMDA receptor (NR1, 2A and 2B subunits) involved in the above behavioral functions was measured in 5 brain regions. B[a]P induced NMDA1 expression in three (hippocampus, amygdala and cerebellum) of five brain regions investigated, and modulated NMDA2 in two of the five brain regions (frontal cortex and cerebellum). Each one of these B[a]P-effects was reversed in mice that were immunized against this PAH, with measurable consequences on behavior such as anxiety, short term learning and memory. Thus active immunization against B[a]P with a B[a]P–DT conjugate vaccine had a protective effect and attenuated the pharmacological and neurotoxic effects even of high concentrations of B[a]P. - Highlights: • B[a]P-antibodies attenuated B[a]P induced NMDA expression in several brain regions. • B[a]P had measurable consequences on anxiety, short term learning and memory. • B[a]P immunization attenuated the pharmacological and neurotoxic effects of B[a]P. • Vaccination may also provide some protection against chemical carcinogenesis.

  3. Aerosol light absorption, black carbon, and elemental carbon at the Fresno Supersite, California

    Science.gov (United States)

    Chow, Judith C.; Watson, John G.; Doraiswamy, Prakash; Chen, Lung-Wen Antony; Sodeman, David A.; Lowenthal, Douglas H.; Park, Kihong; Arnott, W. Patrick; Motallebi, Nehzat

    2009-08-01

    Particle light absorption ( bap), black carbon (BC), and elemental carbon (EC) measurements at the Fresno Supersite during the summer of 2005 were compared to examine the equivalency of current techniques, evaluate filter-based bap correction methods, and determine the EC mass absorption efficiency (σ ap) and the spectral dependence of bap. The photoacoustic analyzer (PA) was used as a benchmark for in-situ bap. Most bap measurement techniques were well correlated ( r ≥ 0.95). Unadjusted Aethalometer (AE) and Particle Soot Absorption Photometer (PSAP) bap were up to seven times higher than PA bap at similar wavelengths because of absorption enhancement by backscattering and multiple scattering. Applying published algorithms to correct for these effects reduced the differences to 24 and 17% for the AE and PSAP, respectively, at 532 nm. The Multi-Angle Absorption Photometer (MAAP), which accounts for backscattering effects, overestimated bap relative to the PA by 51%. BC concentrations determined by the AE, MAAP, and Sunset Laboratory semi-continuous carbon analyzer were also highly correlated ( r ≥ 0.93) but differed by up to 57%. EC measured with the IMPROVE/STN thermal/optical protocols, and the French two-step thermal protocol agreed to within 29%. Absorption efficiencies determined from PA bap and EC measured with different analytical protocols averaged 7.9 ± 1.5, 5.4 ± 1.1, and 2.8 ± 0.6 m 2/g at 532, 670, and 1047 nm, respectively. The Angström exponent (α) determined from adjusted AE and PA bap ranged from 1.19 to 1.46. The largest values of α occurred during the afternoon hours when the organic fraction of total carbon was highest. Significant biases associated with filter-based measurements of bap, BC, and EC are method-specific. Correcting for these biases must take into account differences in aerosol concentration, composition, and sources.

  4. Automatic and controlled processing and the Broad Autism Phenotype.

    Science.gov (United States)

    Camodeca, Amy; Voelker, Sylvia

    2016-01-30

    Research related to verbal fluency in the Broad Autism Phenotype (BAP) is limited and dated, but generally suggests intact abilities in the context of weaknesses in other areas of executive function (Hughes et al., 1999; Wong et al., 2006; Delorme et al., 2007). Controlled processing, the generation of search strategies after initial, automated responses are exhausted (Spat, 2013), has yet to be investigated in the BAP, and may be evidenced in verbal fluency tasks. One hundred twenty-nine participants completed the Delis-Kaplan Executive Function System Verbal Fluency test (D-KEFS; Delis et al., 2001) and the Broad Autism Phenotype Questionnaire (BAPQ; Hurley et al., 2007). The BAP group (n=53) produced significantly fewer total words during the 2nd 15" interval compared to the Non-BAP (n=76) group. Partial correlations indicated similar relations between verbal fluency variables for each group. Regression analyses predicting 2nd 15" interval scores suggested differentiation between controlled and automatic processing skills in both groups. Results suggest adequate automatic processing, but slowed development of controlled processing strategies in the BAP, and provide evidence for similar underlying cognitive constructs for both groups. Controlled processing was predictive of Block Design score for Non-BAP participants, and was predictive of Pragmatic Language score on the BAPQ for BAP participants. These results are similar to past research related to strengths and weaknesses in the BAP, respectively, and suggest that controlled processing strategy use may be required in instances of weak lower-level skills. PMID:26652842

  5. Effects on specific promoter DNA methylation in zebrafish embryos and larvae following benzo[a]pyrene exposure

    Science.gov (United States)

    Benzo[a]pyrene (BaP) is an established reproductive and developmental toxicant. BaP exposure in humans and animals has been linked to infertility and multigenerational health consequences. DNA methylation is the most studied epigenetic mechanism that regulates gene expression, and mapping of methyla...

  6. Grr1p is required for transcriptional induction of amino acid permease genes and proper transcriptional regulation of genes in carbon metabolism of Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Eckert-Boulet, Nadine; Regenberg, Birgitte; Nielsen, Jens

    2005-01-01

    and a grr1 Delta strain and adding citrulline in the exponential phase. Whole-genome transcription analyses were performed on samples from each cultivation, both immediately before and 30 min after citrulline addition. Transcriptional induction of the AAP genes AGP1, BAP2, BAP3, DIP5, GNP1 and TAT1 is fully...

  7. Broader Autism Phenotype and Nonverbal Sensitivity: Evidence for an Association in the General Population

    Science.gov (United States)

    Ingersoll, Brooke

    2010-01-01

    This study examined the relationship between characteristics of the Broader Autism Phenotype (BAP) and nonverbal sensitivity, the ability to interpret nonverbal aspects of communication, in a non-clinical sample of college students. One hundred and two participants completed a self-report measure of the BAP, the Autism Spectrum Quotient (AQ), and…

  8. The Eyes Have It: Visual Help for Auditory Acuity.

    Science.gov (United States)

    Prince, Albert

    A non-aversive modification of Bekesy Audiometric Procedures (BAP) used in assessing hearing ability has been found to produce interpretable audiograms from children whose responses to the unmodified BAP were erratic and uninterpretable. In an experiment with six 8-year-old children, three subjects were assigned to a condition in which a light cue…

  9. Protective effects of green tea polyphenols against benzo[a]pyrene-induced reproductive and trans-generational toxic effects in Japanese Medaka (Oryzias latipes)

    NARCIS (Netherlands)

    Song, Chuankui; Wang, Yanli; Xiao, Zhengcao; Xiao, Bin

    2015-01-01

    In order to investigate the protective effect of green tea polyphenols (GTP) on benzo[a]pyrene (BaP)-induced reproductive and trans-generational toxicity, Japanese Medaka was injected intraperitoneally with BaP alone and co-injected with both BaP and GTP of different concentrations, respectively.

  10. Alexithymia in Parents of Children with Autism Spectrum Disorder

    Science.gov (United States)

    Szatmari, Peter; Georgiades, Stelios; Duku, Eric; Zwaigenbaum, Lonnie; Goldberg, Jeremy; Bennett, Terry

    2008-01-01

    Given the recent findings regarding the association between alexithymia and Autism Spectrum Disorder (ASD) and the accumulating evidence for the presence of the Broader Autism Phenotype (BAP) in relatives of individuals with ASD, we further explored the construct of alexithymia in parents of children with ASD as a potential part of the BAP. We…

  11. Transcriptomic changes in zebrafish embryos and larvae following benzo[a]pyrene exposure

    Science.gov (United States)

    Benzo[a]pyrene (BaP) is an environmentally relevant carcinogenic and endocrine disrupting compound that causes immediate, long-term, and multigenerational health deficits in mammals and fish. Previously, we found that BaP alters DNA methylation patterns in developing zebrafish, which may affect gene...

  12. Model Invariance across Genders of the Broad Autism Phenotype Questionnaire

    Science.gov (United States)

    Broderick, Neill; Wade, Jordan L.; Meyer, J. Patrick; Hull, Michael; Reeve, Ronald E.

    2015-01-01

    ASD is one of the most heritable neuropsychiatric disorders, though comprehensive genetic liability remains elusive. To facilitate genetic research, researchers employ the concept of the broad autism phenotype (BAP), a milder presentation of traits in undiagnosed relatives. Research suggests that the BAP Questionnaire (BAPQ) demonstrates…

  13. Changes in Bone Alkaline Phosphatase and Procollagen Type-1 C-Peptide after Static and Dynamic Exercises

    Science.gov (United States)

    Kubo, Keitaro; Yuki, Kazuhito; Ikebukuro, Toshihiro

    2012-01-01

    We investigated the effects of two types of nonweight-bearing exercise on changes in bone-specific alkaline phosphatase (BAP) and pro-collagen type 1 C-peptide (P1P). BAP is a specific marker of bone synthesis, whereas P1P reflects synthesis of type 1 collagen in other organs as well as bone. Eight participants performed static and dynamic…

  14. Prognostic value of PINP, bone alkaline phosphatase, CTX-I, and YKL-40 in patients with metastatic prostate carcinoma

    DEFF Research Database (Denmark)

    Brasso, Klaus; Christensen, Ib Jarle; Johansen, Julia S;

    2006-01-01

    To examine the prognostic value of markers of bone metabolism (serum PINP, BAP, and CTX-I) and serum YKL-40 in metastatic prostate carcinoma (PC).......To examine the prognostic value of markers of bone metabolism (serum PINP, BAP, and CTX-I) and serum YKL-40 in metastatic prostate carcinoma (PC)....

  15. Association between SNPs within candidate genes and compounds related to boar taint and reproduction

    DEFF Research Database (Denmark)

    Moe, Maren; Lien, Sigbjørn; Aasmundstad, Torunn;

    2009-01-01

    receptor NGFIB, catenin CTNND1, BRCA1 associated protein BAP1 and hyaluronoglucosaminidase HYAL2. Haplotype analysis provided additional evidence for an effect of CYP2E1 on levels of skatole and indole, and for BAP1, HYAL2 and SRD5A2 on levels of androstenone. CONCLUSION: The findings in this study...

  16. New Interview and Observation Measures of the Broader Autism Phenotype: Group Differentiation

    Science.gov (United States)

    de Jonge, Maretha; Parr, Jeremy; Rutter, Michael; Wallace, Simon; Kemner, Chantal; Bailey, Anthony; van Engeland, Herman; Pickles, Andrew

    2015-01-01

    To identify the broader autism phenotype (BAP), the Family History Interview subject and informant versions and an observational tool (Impression of Interviewee), were developed. This study investigated whether the instruments differentiated between parents of children with autism, and parents of children with Down syndrome (DS). The BAP scores of…

  17. 75 FR 3273 - Request for OMB Clearance of an Information Collection Renewal

    Science.gov (United States)

    2010-01-20

    ... 60-day comment period, (74 FR 60012) Docket DOT-OST-2009-0294. The collection is in regards to the... Program (DBE ARRA BAP) application form. The notice was to inform the public of OSDBU's intention to... project. Under the DBE ARRA BAP program, DBEs performing on a transportation and infrastructure...

  18. Brown-like adipose progenitors derived from human induced pluripotent stem cells: Identification of critical pathways governing their adipogenic capacity

    Science.gov (United States)

    Hafner, Anne-Laure; Contet, Julian; Ravaud, Christophe; Yao, Xi; Villageois, Phi; Suknuntha, Kran; Annab, Karima; Peraldi, Pascal; Binetruy, Bernard; Slukvin, Igor I.; Ladoux, Annie; Dani, Christian

    2016-01-01

    Human induced pluripotent stem cells (hiPSCs) show great promise for obesity treatment as they represent an unlimited source of brown/brite adipose progenitors (BAPs). However, hiPSC-BAPs display a low adipogenic capacity compared to adult-BAPs when maintained in a traditional adipogenic cocktail. The reasons of this feature are unknown and hamper their use both in cell-based therapy and basic research. Here we show that treatment with TGFβ pathway inhibitor SB431542 together with ascorbic acid and EGF were required to promote hiPSCs-BAP differentiation at a level similar to adult-BAP differentiation. hiPSC-BAPs expressed the molecular identity of adult-UCP1 expressing cells (PAX3, CIDEA, DIO2) with both brown (ZIC1) and brite (CD137) adipocyte markers. Altogether, these data highlighted the critical role of TGFβ pathway in switching off hiPSC-brown adipogenesis and revealed novel factors to unlock their differentiation. As hiPSC-BAPs display similarities with adult-BAPs, it opens new opportunities to develop alternative strategies to counteract obesity. PMID:27577850

  19. Brown-like adipose progenitors derived from human induced pluripotent stem cells: Identification of critical pathways governing their adipogenic capacity.

    Science.gov (United States)

    Hafner, Anne-Laure; Contet, Julian; Ravaud, Christophe; Yao, Xi; Villageois, Phi; Suknuntha, Kran; Annab, Karima; Peraldi, Pascal; Binetruy, Bernard; Slukvin, Igor I; Ladoux, Annie; Dani, Christian

    2016-01-01

    Human induced pluripotent stem cells (hiPSCs) show great promise for obesity treatment as they represent an unlimited source of brown/brite adipose progenitors (BAPs). However, hiPSC-BAPs display a low adipogenic capacity compared to adult-BAPs when maintained in a traditional adipogenic cocktail. The reasons of this feature are unknown and hamper their use both in cell-based therapy and basic research. Here we show that treatment with TGFβ pathway inhibitor SB431542 together with ascorbic acid and EGF were required to promote hiPSCs-BAP differentiation at a level similar to adult-BAP differentiation. hiPSC-BAPs expressed the molecular identity of adult-UCP1 expressing cells (PAX3, CIDEA, DIO2) with both brown (ZIC1) and brite (CD137) adipocyte markers. Altogether, these data highlighted the critical role of TGFβ pathway in switching off hiPSC-brown adipogenesis and revealed novel factors to unlock their differentiation. As hiPSC-BAPs display similarities with adult-BAPs, it opens new opportunities to develop alternative strategies to counteract obesity. PMID:27577850

  20. The Autism-Spectrum Quotient--Italian Version: A Cross-Cultural Confirmation of the Broader Autism Phenotype

    Science.gov (United States)

    Ruta, Liliana; Mazzone, Domenico; Mazzone, Luigi; Wheelwright, Sally; Baron-Cohen, Simon

    2012-01-01

    The Autism Spectrum Quotient (AQ) has been used to define the "broader" (BAP), "medium" (MAP) and "narrow" autism phenotypes (NAP). We used a new Italian version of the AQ to test if difference on AQ scores and the distribution of BAP, MAP and NAP in autism parents (n = 245) versus control parents (n = 300) were replicated in a Sicilian sample.…

  1. New Interview and Observation Measures of the Broader Autism Phenotype : Description of Strategy and Reliability Findings for the Interview Measures

    NARCIS (Netherlands)

    Parr, Jeremy R.; De Jonge, Maretha V.; Wallace, Simon; Pickles, Andrew; Rutter, Michael L.; Le Couteur, Ann S.; van Engeland, Herman; Wittemeyer, Kerstin; Mcconachie, Helen; Roge, Bernadette; Mantoulan, Carine; Pedersen, Lennart; Isager, Torben; Poustka, Fritz; Bolte, Sven; Bolton, Patrick; Weisblatt, Emma; Green, Jonathan; Papanikolaou, Katerina; Baird, Gillian; Bailey, Anthony J.

    2015-01-01

    Clinical genetic studies confirm the broader autism phenotype (BAP) in some relatives of individuals with autism, but there are few standardized assessment measures. We developed three BAP measures (informant interview, self-report interview, and impression of interviewee observational scale) and de

  2. Evaluation of the health risk resulting from exposure to polycyclic aromatic hydrocarbons in coal-tar shampoo

    NARCIS (Netherlands)

    Mennes WC; van Veen MP; Kroese ED; Speijers GJA; ACT; LEO; LBO

    1996-01-01

    Omdat verscheidene PAK, waaronder benzo(a)pyrene (BaP) worden beschouwd als carcinogene stoffen, zijn er vraagtekens gesteld bij de veiligheid van deze producten. Een schatting van de externe dermale blootstelling aan BaP is afgeleid door toepassing van een mathematisch model. Deze schatting was h

  3. Evaluation of the health risk resulting from exposure to polycyclic aromatic hydrocarbons in coal-tar shampoo

    NARCIS (Netherlands)

    Mennes WC; Veen MP van; Kroese ED; Speijers GJA; ACT; LEO; LBO

    1996-01-01

    Because several PAHs, among which benzo(a)pyrene (BaP), are considered to be carcinogenic substances, the safety of these products has been questioned. An estimate of external dermal exposure to BaP has been generated by application of a mathematical model. This estimate was the starting point in an

  4. Benzo(a)pyrene induces oxidative stress, pro-inflammatory cytokines, expression of nuclear factor-kappa B and deregulation of wnt/beta-catenin signaling in colons of BALB/c mice.

    Science.gov (United States)

    Ajayi, Babajide O; Adedara, Isaac A; Farombi, Ebenezer O

    2016-09-01

    The incidence of colonic toxicity has been epidemiologically linked to the consumption of foods contaminated with benzo(a)pyrene (B[a]P). The present study investigated the effects of B[a]P on biomarkers of oxidative stress, inflammation and wnt-signaling in colon of BALB/c mice following exposure to 62.5, 125 and 250 mg/kg of B[a]P for 7 days by oral gavage. Exposure to B[a]P significantly decreased the colonic antioxidant enzymes activities and glutathione level with concomitant significant increase in myeloperoxidase activity, nitric oxide and lipid peroxidation levels. Colon histopathology results showed treatment-related lesions characterized by atrophy, mucosal ulceration and gland erosion in the B[a]P-treated mice. Immunohistochemistry analysis showed that B[a]P treatment increased the protein expression of nuclear factor kappa B, pro-inflammatory cytokines namely tumor necrosis factor alpha and interleukin-1β, as well as cyclooxygenase-2 and inducible nitric oxide synthase in the mice colon. Altered canonical wnt-signaling was confirmed by strong diaminobenzidine staining for p38 mitogen activated protein kinase, β-catenin expression and absence of adenomatous polyposis coli following B[a]P administration. The present data highlight that exposure to B[a]P induces colon injury via induction of oxidative and nitrosative stress, inflammatory biomarkers and dsyregulation wnt/β-catenin signaling, thus confirming the role of B[a]P in the pathogenesis of colonic toxicity. PMID:27338711

  5. Using gridded multimedia model to simulate spatial fate of Benzo[α]pyrene on regional scale.

    Science.gov (United States)

    Liu, Shijie; Lu, Yonglong; Wang, Tieyu; Xie, Shuangwei; Jones, Kevin C; Sweetman, Andrew J

    2014-02-01

    Predicting the environmental multimedia fate is an essential step in the process of assessing the human exposure and health impacts of chemicals released into the environment. Multimedia fate models have been widely applied to calculate the fate and distribution of chemicals in the environment, which can serve as input to a human exposure model. In this study, a grid based multimedia fugacity model at regional scale was developed together with a case study modeling the fate and transfer of Benzo[α]pyrene (BaP) in Bohai coastal region, China. Based on the estimated emission and in-site survey in 2008, the BaP concentrations in air, vegetation, soil, fresh water, fresh water sediment and coastal water as well as the transfer fluxes were derived under the steady-state assumption. The model results were validated through comparison between the measured and modeled concentrations of BaP. The model results indicated that the predicted concentrations of BaP in air, fresh water, soil and sediment generally agreed with field observations. Model predictions suggest that soil was the dominant sink of BaP in terrestrial systems. Flow from air to soil, vegetation and costal water were three major pathways of BaP inter-media transport processes. Most of the BaP entering the sea was transferred by air flow, which was also the crucial driving force in the spatial distribution processes of BaP. The Yellow River, Liaohe River and Daliao River played an important role in the spatial transformation processes of BaP. Compared with advection outflow, degradation was more important in removal processes of BaP. Sensitivities of the model estimates to input parameters were tested. The result showed that emission rates, compartment dimensions, transport velocity and degradation rates of BaP were the most influential parameters for the model output. Monte Carlo simulation was carried out to determine parameter uncertainty, from which the coefficients of variation for the estimated BaP

  6. Overexpression of Catalase Enhances Benzo(a)pyrene Detoxification in Endothelial Microsomes

    Science.gov (United States)

    Yang, Fang; Yang, Hong; Ramesh, Aramandla; Goodwin, J. Shawn; Okoro, Emmanuel U.; Guo, ZhongMao

    2016-01-01

    We previously reported that overexpression of catalase upregulated xenobiotic- metabolizing enzyme (XME) expression and diminished benzo(a)pyrene (BaP) intermediate accumulation in mouse aortic endothelial cells (MAECs). Endoplasmic reticulum (ER) is the most active organelle involved in BaP metabolism. To examine the involvement of ER in catalase-induced BaP detoxification, we compared the level and distribution of XMEs, and the profile of BaP intermediates in the microsomes of wild-type and catalase transgenic endothelial cells. Our data showed that endothelial microsomes were enriched in cytochrome P450 (CYP) 1A1, CYP1B1 and epoxide hydrolase 1 (EH1), and contained considerable levels of NAD(P)H: quinone oxidoreductase-1 (NQO1) and glutathione S-transferase-pi (GSTP). Treatment of wild-type MAECs with 1μM BaP for 2 h increased the expression of microsomal CYP1A1, 1B1 and NQO1 by ~300, 64 and 116%, respectively. However, the same treatment did not significantly alter the expression of EH1 and GSTP. Overexpression of catalase did not significantly increase EH1, but upregulated BaP-induced expression of microsomal CYP1A1, 1B1, NQO1 and GSTP in the following order: 1A1>NQO1>GSTP>1B1. Overexpression of catalase did not alter the distribution of each of these enzymes in the microsomes. In contrast to our previous report showing lower level of BaP phenols versus BaP diols/diones in the whole-cell, this report demonstrated that the sum of microsomal BaP phenolic metabolites were ~60% greater than that of the BaP diols/diones after exposure of microsomes to BaP. Overexpression of catalase reduced the concentrations of microsomal BaP phenols and diols/diones by ~45 and 95%, respectively. This process enhanced the ratio of BaP phenol versus diol/dione metabolites in a potent manner. Taken together, upregulation of phase II XMEs and CYP1 proteins, but not EH1 in the ER might be the mechanism by which overexpression of catalase reduces the levels of all the BaP metabolites

  7. Período de formação do cacho e biorreguladores na produção e qualidade da banana 'Grande Naine'

    Directory of Open Access Journals (Sweden)

    Juliana Domingues Lima

    2015-08-01

    Full Text Available Objetivou-se determinar o efeito do período de formação do cacho e dos biorreguladores na produção e qualidade da banana 'Grande Naine'. O experimento foi realizado em delineamento inteiramente casualizado em parcelas subdivididas, com seis repetições. Consideram-se, nas parcelas, os períodos de formação do cacho, verão e outono, e nas subparcelas, os biorreguladores: duas aplicações com água, giberelina (AG3, 200mg L-1, auxina (2,4 D; 10mg L-1, citocinina (TDZ, 150mg L-1 e a mistura de giberelina, auxina e citocinina (AG3, 56,3mg L-1; AIB, 56,3mg L-1 e ZEA, 101,3mg L-1. Os períodos de formação do cacho influenciam o número de pencas, o tamanho, a vida pós-colheita, o pH, a acidez titulável, a razão sólidos solúveis e acidez titulável e a firmeza do fruto. A mistura de biorreguladores eleva o peso do cacho e da 4ª penca em cachos formados no verão.

  8. The research of cytokinin and hydrogen peroxide influence on the leaf mesophyll structure and morphometric parameters of winter wheat

    Directory of Open Access Journals (Sweden)

    Vladislav V. Zhuk

    2013-04-01

    Full Text Available The last leaf mesophyll structure of Triticum aestivumcv. ‘Tronka’which was grown in the field experiment conditions was investigated by the method of confocal laser scanning microscopy. The experiment plants were treated by BAP and Н 2О2 in booting phase. It is established that plant treatment didn’t effect on structure of leaf tissues but enhanced the functional ability of chloroplast pigment complexes. It has shown that influence of exogenous BAP enhanced chlorophyll autofluorescence, especially in palisade mesophyll cells, delayed aging of pigment complex. The action of exogenous mainly increased chlorophyll fluorescence of spongy mesophyll near stomata, but its influence was less significant than BAP. In winter wheat plants treated by BAP and Н 2О2 plant height and last leaf length enlarged. The plant treatment by BAP increased productivity of T. aestivumcv. ‘Tronka’ mainly by the cost of increasing of corns’ quantity in ear and corns’weight.

  9. Significant Association Between Bone-Specific Alkaline Phosphatase and Vascular Calcification of the Hand Arteries in Male Hemodialysis Patients

    Directory of Open Access Journals (Sweden)

    Eiji Ishimura

    2014-09-01

    Full Text Available Background/Aims: Bone-specific alkaline phosphatase (BAP hydrolyzes pyrophosphate, which inhibits vascular calcification. We examined association between serum BAP and vascular calcification of male hemodialysis patients. Methods: Hand roentgenography of 167 male maintenance hemodialysis patients was conducted, and visible vascular calcification of the hand arteries was evaluated. Serum levels of 3 bone formation markers (BAP, osteocalcin, and N-terminal propeptide of type I collagen and 2 bone resorption markers (C-terminal telopeptide of type I collagen, and cross-linked N-telopeptide of type I collagen were measured, along with serum intact parathyroid hormone (PTH. Results: Of 167 patients, visible vascular calcification was seen in 37 patients. Among the bone formation and resorption markers, serum BAP was significantly higher in patients with vascular calcification than in those without (pConclusions: Higher serum BAP, but not other bone markers, is significantly associated with the presence of vascular calcification in male hemodialysis patients.

  10. Cytochrome P4501A induction, benzo[a]pyrene metabolism, and nucleotide adduct formation in fish hepatoma cells: Effect of preexposure to 3,3',4,4',5-pentachlorobiphenyl

    Science.gov (United States)

    Smeets, J.M.W.; Voormolen, A.; Tillitt, D.E.; Everaarts, J.M.; Seinen, W.; Vanden Berg, M.D.

    1999-01-01

    In PLHC-1 hepatoma cells, benzo[a]pyrene (B[a]P) caused a maximum induction of cytochrome P4501A (CYP1A) activity, measured as ethoxyresorufin O-deethylation (EROD), after 4 to 8 h of exposure, depending on the B[a]P concentration. The decline of EROD activity at longer exposure times was probably caused by the rapid metabolism of B[a]P in this system (57% metabolism within 4 h incubation). In subsequent experiments, PLHC-1 cells were preinduced with PCB 126 for 24 h and then received a dose of 10, 100, or 1,000 nM 3H-B[a]P. A 1-nM concentration of PCB 126 caused an 80-fold induction of CYP1A activity, resulting in an increase in B[a]P metabolism of less than 10%, except at the highest concentration of B[a]P (1,000 nM), where a 50% increase was observed. In another experiment, an 80-fold induction of CYP1A activity caused a 20% increase in the metabolism of B[a]P (100 nM), and RNA adduct formation was increased approximately twofold. These results indicate that, at exposure concentrations up to 100 nM B[a]P, CYP1A activity is not rate limiting for B[a]P metabolism. Furthermore, CYP1A seems to also he specifically involved in B[a]P activation in PLHC-1 cells. However, CYP1A induction causes only a relatively small increase in activation, probably because of the action of other enzymes involved in B[a]P activation and deactivation.

  11. Effects of polycyclic aromatic hydrocarbons (PAHs) on an aquatic ecosystem: acute toxicity and community-level toxic impact tests of benzo[a]pyrene using lake zooplankton community.

    Science.gov (United States)

    Ikenaka, Yoshinori; Sakamoto, Masaki; Nagata, Takamaru; Takahashi, Hirokazu; Miyabara, Yuichi; Hanazato, Takayuki; Ishizuka, Mayumi; Isobe, Tomohiko; Kim, Jun-Woo; Chang, Kwang-Hyeon

    2013-02-01

    We estimated acute toxicity of benzo[a]pyrene (B[a]P) using two cladoceran species, Ceriodaphnia reticulata and Daphnia magna, and also analyzed its impact on zooplankton community throughout an exposure experiment using small-scale mesocosms. LC(50) of B[a]P for C. reticulata and D. magna was 4.3 and 4.7 µg/l, respectively. However, individuals fed with Chlorella showed higher LC(50), 6.1 µg/l for C. reticulata and 8.0 µg/l for D. magna. In the exposure experiment, we examined the impact of B[a]P on zooplankton community using conceivable concentrations in the environment (5 and 10 µg/l) using typical zooplankton community in eutrophicated systems. Despite the residence time of B[a]P in the water column was short as recovery pattern was different among cladocerans and rotifers. Consequently, B[a]P showed insecticide-like impacts, suppressing cladoceran populations and inducing the dominance of rotifers particularly under high concentration (10 µg/l). Results have suggested that, even such short duration of B[a]P in the water body can have impact on zooplankton abundance and community structure. Since B[a]P easily precipitate to the bottom and rapidly disappears from the water body, careful monitoring and further assessment of the potential toxicity of polycyclic aromatic hydrocarbons are necessary. PMID:23358147

  12. Relation of hepatic EROD activity and CYP1A level in Sebastiscus marmoratus exposed to benzo[a]pyrene

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    This study was designed to investigate the in vivo effects of benzo[a]pyrene (BaP) on hepatic ethoxyresorufin-O-deethylase (EROD) activity and its correlation with cytochrome P450 1A (CYP1A) protein levels in Sebastiscus marmoratus, which were exposed through a water column to BaP (10, 100, 1000 ng/L, respectively) or were treated with intraperitoneal injections of BaP (0.5, 1, 5, 10 mg/kg, respectively) every 7 d. The results showed that after 25 d of waterborne exposure to 1000 ng/L BaP, fish hepatic CYP1A levels and EROD activity were significantly induced. In contrast, EROD activity was not altered 7 d after second ip injections, whereas, CYP1A protein levels were increased. Dose-dependent increase of biliary BaP metabolites demonstrated that the catalytic activity of CYP1A was induced by treatment with BaP. The lowest observable effect concentration with regard to biliary BaP metabolites (100 ng/L) was much lower than that with reference to EROD activity (1000 ng/L). The results suggest that biliary polycyclic aromatic hydrocarbon (PAH) metabolites were shown to better reflect the contamination gradients of PAHs than EROD activity. It appeared to be necessary to measure CYP1A protein levels to complement the EROD activity in relevant toxicological assessments.

  13. Degradation of 14C labelled Benzo[a]pyrene by a PAH-adapted mixed bacterial culture in the presence of an alkylpolyglycoside-surfactant

    International Nuclear Information System (INIS)

    The biodegradation of the five ring PAH benzo[a]pyrene (BaP) is assumed to be limited by the low water solubility of this compound. A mixed culture of microorganisms - isolated from a PAH-contaminated soil - was able to degrade 14C labelled BaP in mineral medium by co-metabolism with phenanthrene, fluoranthene, anthracene and pyrene as sources of carbon and energy. The mineralisation of these compounds to low levels resulted in an inhibition of the degradation of BaP. After the new addition of the four PAH compounds to the culture medium the mineralisation of BaP started again. A non-ionic surfactant of the alkylpolyglycoside type (Plantacare 2000 UP) increased the concentration of BaP in the culture medium because of solubilization. At high Plantacare concentrations, the degradation of BaP was completely inhibited above the critical micelle concentration (cms). The degradation of the three and four ring PAHs was also inhibited. If the surfactant was metabolised to concentrations below the cmc, an increase of mineralisation of BaP could occur up to 24% in 384 days. (orig.)

  14. Estimation of a lifetime unit lung cancer risk for benzo(a)pyrene based on tumour rates in rats exposed to coal tar/pitch condensation aerosol.

    Science.gov (United States)

    Heinrich, U; Roller, M; Pott, F

    1994-06-01

    Female Wistar rats were exposed to coal tar/pitch condensation (CTP) aerosol containing either 20 or 46 micrograms/m3 benzo(a)pyrene (BaP) among other polycyclic aromatic hydrocarbons (PAH) 17 h/day and 5 days/week for 10 or 20 months followed by a clean air period of up to 20 or 10 months, respectively. Based on the inhaled BaP, given as BaP exposure concentration multiplied by the total exposure time, the cumulative dose of inhaled BaP of the 4 exposure groups was 71, 142, 158 and 321 mg BaP/m3 x h and the corresponding lung tumour rates were 4.2, 33.3, 38.9 and 97.2%. There was no lung tumour in the control group. Using the US Environmental Protection Agency (EPA) linearized multistage model, the lifetime lung tumour risk for rats exposed to 1 microgram/m3 BaP as a constituent of a complex PAH mixture may be 2% or correspondingly 2 per 100,000 with a BaP concentration of 1 ng/m3. The estimation of the unit lung cancer risk for BaP based on epidemiological data from coking plants was 7-9%.

  15. Biodegradation of Benzo[a]pyrene by Arthrobacter oxydans B4

    Institute of Scientific and Technical Information of China (English)

    PENG Hui; YIN Hua; DENG Jun; YE Jin-Shao; CHEN Shuo-Na; HE Bao-Yan; ZHANG Na

    2012-01-01

    A bacterial strain,Arthrobacter oxydans (B4),capable of degrading benzo[a]pyrene (BaP) in water body,was isolated from a polycyclic aromatic hydrocarbons-contaminated site.Effects of different factors,such as reaction time,pH value,temperature and organic nutrients,on BaP biodegradation by the strain B4 were studied.After 5 d treatment,the concentration of BaP in mineral salts medium was reduced to 0.318 mg L-1,compared to the initial concentration of 1.000 mg L-1.There was a process of acid formation during the degradation with pH failing from initial 7.01 to 4.61 at 5 d,so keeping the water body under slightly alkaline condition was propitious to BaP degradation.Strain B4 efficiently degraded BaP at 20 to 37 ℃ with addition of organic nutrients.The biodegradation and transformation of BaP mainly occurred on cell surfaces,and extracellular secretions played an important role in these processes.Fourier transform infrared spectroscopy and gas chromatograph-mass spectrometer analyses of metabolites showed that ring cleavage occurred in the BaP degradation process and the resulting metabolically utilizable substrates were generated as sole carbon sources for B4 growth.Furthermore,mineralization extent of metabolites was verified by determining the total organic carbon and inorganic carbon in the degradation system.

  16. Mouth-Level Intake of Benzo[a]pyrene from Reduced Nicotine Cigarettes

    Directory of Open Access Journals (Sweden)

    Yan S. Ding

    2014-11-01

    Full Text Available Cigarette smoke is a known source of exposure to carcinogenic polycyclic aromatic hydrocarbons (PAHs, especially benzo[a]pyrene (BaP. Exposure to BaP in cigarette smoke is influenced by how a person smokes and factors, such as tobacco blend. To determine whether sustained use of reduced-nicotine cigarettes is associated with changes in exposure to nicotine and BaP, levels of BaP in spent cigarette filter butts were correlated with levels of BaP in cigarette smoke to estimate mouth-level intake (MLI of BaP for 72 daily smokers given three progressively reduced nicotine content cigarettes. Urinary cotinine, a marker of nicotine exposure, and urinary 1-hydroxypyrene (1-HOP, a marker of PAH exposure, were measured throughout the study. Median daily BaP MLI and urine cotinine decreased in a similar manner as smokers switched to progressively lower nicotine cigarettes, despite relatively constant daily cigarette consumption. 1-HOP levels were less responsive to the use of reduced nicotine content cigarettes. We demonstrate that spent cigarette filter butt analysis is a promising tool to estimate MLI of harmful chemicals on a per cigarette or per-day basis, which partially addresses the concerns of the temporal influence of smoking behavior or differences in cigarette design on exposure.

  17. A potential biomarker for fatigue: Oxidative stress and anti-oxidative activity.

    Science.gov (United States)

    Fukuda, Sanae; Nojima, Junzo; Motoki, Yukari; Yamaguti, Kouzi; Nakatomi, Yasuhito; Okawa, Naoko; Fujiwara, Kazumi; Watanabe, Yasuyoshi; Kuratsune, Hirohiko

    2016-07-01

    We sought to determine whether oxidative stress and anti-oxidative activity could act as biomarkers that discriminate patients with chronic fatigue syndrome (CFS) from healthy volunteers at acute and sub-acute fatigue and resting conditions. We calculated the oxidative stress index (OSI) from reactive oxygen metabolites-derived compounds (d-ROMs) and the biological antioxidant potential (BAP). We determined changes in d-ROMs, BAP, and OSI in acute and sub-acute fatigue in two healthy groups, and compared their values at rest between patients with CFS (diagnosed by Fukuda 1994 criteria) and another group of healthy controls. Following acute fatigue in healthy controls, d-ROMs and OSI increased, and BAP decreased. Although d-ROMs and OSI were significantly higher after sub-acute fatigue, BAP did not decrease. Resting condition yielded higher d-ROMs, higher OSI, and lower BAP in patients with CFS than in healthy volunteers, but lower d-ROMs and OSI when compared with sub-acute controls. BAP values did not significantly differ between patients with CFS and controls in the sub-acute condition. However, values were significantly higher than in the resting condition for controls. Thus, measured of oxidative stress (d-ROMS) and anti-oxidative activity (BAP) might be useful for discriminating acute, sub-acute, and resting fatigue in healthy people from patients with CFS, or for evaluating fatigue levels in healthy people.

  18. Photochemical behavior of benzo[a]pyrene on soil surfaces under UV light irradiation

    Institute of Scientific and Technical Information of China (English)

    ZHANG Li-hong; LI Pei-jun; GONG Zong-qiang; Oni Adeola A.

    2006-01-01

    The rates of photodegradation and photocatalysis of benzo [a]pyrene (BaP) on soil surfaces under UV light have been studied. Different parameters such as temperature, soil particle sizes, and soil depth responsible for photodegradation, catalyst loads and wavelength of UV irradiation blamed for photocatalysis have been monitored. The results obtained indicated that BaP photodegradation follows pseudo-first-order kinetics. BaP photodegradation was the fastest at 30℃. The rates of BaP photodegradation at different soil particle size followed the order: less than 1 mm>less than 0.45 mm>less than 0.25 mm. When the soil depth increased from 1 mm to 4 mm, the half-life increased from 13.23 d to 17.73 d. The additions of TiO2 or Fe2O3 accelerated the photodegradation of BaP, and the photocatalysis of BaP follows pseudo-first-order kinetics. Changes in catalyst loads of TiO2 (0.5%,1%, 2%, and 3% (wt)) or Fe2O3 (2%, 5%, 7%, and 10% (wt)) did not significantly affect the degradation rates. Both BaP photocatalysis in the presence of TiO2 and Fe2O3 were the fastest at 254 nm UV irradiation.

  19. Biophysicochemical characterization of an alkaline protease from Beauveria sp. MTCC 5184 with multiple applications.

    Science.gov (United States)

    Shankar, Shiv; Laxman, Ryali Seeta

    2015-01-01

    This study illustrates the biophysicochemical properties of an alkaline protease, BAP (Beauveria sp. alkaline protease) from Beauveria sp. MTCC 5184. This protease exhibited maximum activity at 50 °C, pH 9.0, and stability in a broad pH range, in the presence of organic solvents, denaturants, as well as detergents. Wash performance studies revealed that BAP was able to remove blood clots/stains from blood-soaked cloth. Peptide mass fingerprinting results demonstrated partial homology of BAP with subtilisin-like proteinase. BAP showed catalytic activity against natural as well as synthetic substrates. Active site characterization of BAP confirmed the involvement of serine, tryptophan, and aspartic acid in catalytic activity. Detailed kinetic and thermodynamic studies of BAP demonstrated that the activation energy (Ea) for casein hydrolysis was 82.55 kJ/M, the specificity constant (Kcat/K m), and the values of ∆G (change in Gibbs free energy) decreased with increase in temperature, whereas ∆H (change in enthalapy) and ∆S (change in entropy) were constant. The results of the present study indicate that BAP has potential for applications as detergent additive, in peptide synthesis, and in basic research.

  20. BRCA-associated protein 1 mutant cholangiocarcinoma: an aggressive disease subtype

    Science.gov (United States)

    Al-Shamsi, Humaid O.; Anand, Deepa; Shroff, Rachna T.; Jain, Apurva; Zuo, Mingxin; Conrad, Claudius; Vauthey, Jean-Nicolas

    2016-01-01

    Background BRCA-associated protein 1, an enzyme encoded by the BAP1 gene, is commonly mutated in uveal melanoma, mesothelioma, and renal cancers. Tumors with BAP1 mutation follow an aggressive course. BAP1 mutations have also been observed in cholangiocarcinoma (CCA). The clinical phenotype of BAP1 mutant CCA may yield useful prognostic and therapeutic information but has not been defined. Methods The records of CCA patients who underwent next-generation sequencing (NGS) were reviewed, and data on clinical, histopathological, genetic, and radiological features; response to therapy; time to progression; and survival were analyzed. Results Twenty-two cases of BAP1-mutation associated CCA were diagnosed from January 1, 2009, to February 1, 2015, at our center. Twenty patients had intrahepatic CCA and two had extrahepatic CCA. Tumor sizes (largest dimension) ranged from 2 to 16 cm (mean, 8.5 cm). Twelve patients had tumors that were poorly differentiated. Majority of the patients had advanced disease at presentation and 13 had bone metastases. Thirteen patients (59%) experienced rapidly progressive disease following primary therapy (chemotherapy or surgical resection). The mean time to tumor progression was 3.8 months after the first line chemotherapy. Conclusions BAP1 mutation in CCA may be associated with aggressive disease and poor response to standard therapies. Therefore, BAP1-targeted therapies need to be investigated. PMID:27563445

  1. Change of cholinergic transmission and memory deficiency induced by injection of b-amyloid protein into NBM of rats

    Institute of Scientific and Technical Information of China (English)

    马晓峰; 叶惟泠; 梅镇彤

    2001-01-01

    The change of cholinergic transmission of b-amyloid protein (b-AP) treated rats was studied by intracerebral microdialysis sampling combined with HPLC analysis. b-AP1-40 was injected into nucleus basalis magnocellularis (NBM). Passive avoidance response test (step-down test) and delayed alternation task were used for memory testing. The impairment of memory after injection of b-AP1-40 into NBM exhibited mainly the deficiency of short-term working memory. One week after injection of b-AP1-40 the release of acetylcholine (ACh) from frontal cortex of freely-moving rats decreased significantly, and the response of cholinergic nerve ending to the action of high [K+] solution was rather weak. In control animals the percentage of increase of ACh- release during behavioral performance was 57%, while in b-AP1-40 - treated rats it was 34%. The temporary in-crease of the ACh-release of the rat put into a new place was also significantly diminished in b-AP1-40 -treated rats. The results show that the injection of b-AP1-40 into NBM impairs the cholinergic transmission in frontal cortex, and the impairment of cholinergic transmission may be the main cause of the deficit of working memory.

  2. Neonatal Benzo[a]pyrene Exposure Induces Oxidative Stress and DNA Damage Causing Neurobehavioural Changes during the Early Adolescence Period in Rats.

    Science.gov (United States)

    Patel, Bhupesh; Das, Saroj Kumar; Patri, Manorama

    2016-01-01

    Humans are exposed to polycyclic aromatic hydrocarbons (PAHs) by ingestion of contaminated food and water. Prenatal exposure to benzo[a]pyrene (B[a]P) like PAHs through the placental barrier and neonatal exposure by breast milk and the environment may affect early brain development. In the present study, single intracisternal administration of B[a]P (0.2 and 2.0 µg/kg body weight) to male Wistar rat pups at postnatal day 5 (PND5) was carried out to study its specific effect on neonatal brain development and its consequences at PND30. B[a]P administration showed a significant increase in exploratory and anxiolytic-like behaviour with elevated hippocampal lipid peroxidation and protein oxidation at PND30. Further, DNA damage was estimated in vitro (Neuro2a and C6 cell lines) by the comet assay, and oxidative DNA damage of hippocampal sections was measured in vivo following exposure to B[a]P. DNA strand breaks (single and double) significantly increased due to B[a]P at PND30 in hippocampal neurons and increased the nuclear tail moment in Neuro2a cells. Hippocampal 8-oxo-2'-deoxyguanosine production was significantly elevated showing expression of more TUNEL-positive cells in both doses of B[a]P. Histological studies also revealed a significant reduction in mean area and perimeter of hippocampal neurons in rats treated with B[a]P 2.0 μg/kg, when compared to naïve and control rats. B[a]P significantly increased anxiolytic-like behaviour and oxidative DNA damage in the hippocampus causing apoptosis that may lead to neurodegeneration in adolescence. The findings of the present study address the potential role of B[a]P in inducing oxidative stress-mediated neurodegeneration in the hippocampus through oxidative DNA damage in the early adolescence period of rats. PMID:27271523

  3. New insights into BaP-induced toxicity: role of major metabolites in transcriptomics and contribution to hepatocarcinogenesis.

    Science.gov (United States)

    Souza, Terezinha; Jennen, Danyel; van Delft, Joost; van Herwijnen, Marcel; Kyrtoupolos, Soterios; Kleinjans, Jos

    2016-06-01

    Benzo(a)pyrene (BaP) is a ubiquitous carcinogen resulting from incomplete combustion of organic compounds and also present at high levels in cigarette smoke. A wide range of biological effects has been attributed to BaP and its genotoxic metabolite BPDE, but the contribution to BaP toxicity of intermediary metabolites generated along the detoxification path remains unknown. Here, we report for the first time how 3-OH-BaP, 9,10-diol and BPDE, three major BaP metabolites, temporally relate to BaP-induced transcriptomic alterations in HepG2 cells. Since BaP is also known to induce AhR activation, we additionally evaluated TCDD to source the expression of non-genotoxic AhR-mediated patterns. 9,10-Diol was shown to activate several transcription factor networks related to BaP metabolism (AhR), oxidative stress (Nrf2) and cell proliferation (HIF-1α, AP-1) in particular at early time points, while BPDE influenced expression of genes involved in cell energetics, DNA repair and apoptotic pathways. Also, in order to grasp the role of BaP and its metabolites in chemical hepatocarcinogenesis, we compared expression patterns from BaP(-metabolites) and TCDD to a signature set of approximately nine thousand gene expressions derived from hepatocellular carcinoma (HCC) patients. While transcriptome modulation by TCDD appeared not significantly related to HCC, BaP and BPDE were shown to deregulate metastatic markers via non-genotoxic and genotoxic mechanisms and activate inflammatory pathways (NF-κβ signaling, cytokine-cytokine receptor interaction). BaP also showed strong repression of genes involved in cholesterol and fatty acid biosynthesis. Altogether, this study provides new insights into BaP-induced toxicity and sheds new light onto its mechanism of action as a hepatocarcinogen.

  4. Pregnane X receptor protects HepG2 cells from BaP-induced DNA damage.

    Science.gov (United States)

    Naspinski, Christine; Gu, Xinsheng; Zhou, Guo-Dong; Mertens-Talcott, Susanne U; Donnelly, Kirby C; Tian, Yanan

    2008-07-01

    Pregnane X receptor (PXR) is a nuclear receptor that coordinately regulates transcriptional expression of both phase I and phase II metabolizing enzymes. PXR plays an important role in the pharmacokinetics of a broad spectrum of endogenous and xenobiotic compounds and appears to have evolved in part to protect organisms from toxic xenobiotics. Metabolism of benzo[a]pyrene (BaP), a well-established carcinogen and ubiquitous environmental contaminant, can result in either detoxification or bioactivation to its genotoxic forms. Therefore, PXR could modulate the genotoxicity of BaP by changing the balance of the metabolic pathways in favor of BaP detoxification. To examine the role of PXR in BaP genotoxicity, BaP-DNA adduct formation was measured by 32P-postlabeling in BaP-treated parental HepG2 cells and human PXR-transfected HepG2 cells. The presence of transfected PXR significantly reduced the level of adducts relative to parental cells by 50-65% (p BaP. To analyze potential PXR-regulated detoxification pathways in liver cells, a panel of genes involved in phase I and phase II metabolism and excretion was surveyed with real-time quantitative reverse transcription PCR. The messenger RNA levels of CYP1A2, GSTA1, GSTA2, GSTM1, UGT1A6, and BCRP (ABCG2) were significantly higher in cells overexpressing PXR, independent of exposure to BaP. In addition, the total GST enzymatic activity, which favors the metabolic detoxification of BaP, was significantly increased by the presence of PXR (p BaP exposure. Taken together, these results suggest that PXR plays an important role in protection against DNA damage by polycyclic aromatic hydrocarbons (PAHs) such as BaP, and that these protective effects may be through a coordinated regulation of genes involved in xenobiotic metabolism.

  5. Effect of PCB153 on BaP-induced genotoxicity in HepG2 cells via modulation of metabolic enzymes.

    Science.gov (United States)

    Wei, Wei; Zhang, Chi; Liu, Ai-Lin; Xie, Shao-Hua; Chen, Xue-Min; Lu, Wen-Qing

    2009-04-30

    Benzo(a)pyrene (BaP) is a representative environmental carcinogen and is metabolically activated by several cytochrome P450 (CYP) enzymes to become the ultimate carcinogen. Numerous studies have indicated that 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153) could effectively alter the activity of xenobiotic metabolizing enzymes (XMEs). Therefore, we propose that PCB153 may affect BaP-induced genotoxicity mediated by XMEs. In the present study, we treated HepG2 cells with BaP (50 microM) or PCB153 (0.1, 1, 10 and 100 microM), or pretreated the cells with PCB153 for 48 h followed by treatment with a combination of both BaP and PCB153. CYP1A1 activity was dramatically increased in cells treated with either BaP or PCB153. Glutathione-S-transferase (GST) activity was increased in BaP-treated cells, but decreased in PCB153-treated cells. In parallel to studies on enzyme activity, the micronuclei (MN) assay was used to assess the genotoxic damage caused by BaP and PCB153. BaP and PCB153 at 100 microM enhanced MN formation. In contrast to BaP treatment alone, treatment with both BaP and PCB153 significantly enhanced the activity of CYP1A1 and the formation of MN, but reduced the activity of GST. alpha-Naphthoflavone (ANF), an inhibitor of CYP1A1, inhibited MN formation in the presence of both BaP and PCB153. In addition, there was a positive correlation between CYP1A activity and MN formation (r(2)=0.794, PBaP and PCB153 may increase BaP-induced genotoxicity, possibly through the induction of CYP1A1 and inhibition of GST.

  6. Neonatal exposure to benzo[a]pyrene induces oxidative stress causing altered hippocampal cytomorphometry and behavior during early adolescence period of male Wistar rats.

    Science.gov (United States)

    Patel, Bhupesh; Das, Saroj Kumar; Das, Swagatika; Das, Lipsa; Patri, Manorama

    2016-05-01

    Environmental neurotoxicants like benzo[a]pyrene (B[a]P) have been well documented regarding their potential to induce oxidative stress. However, neonatal exposure to B[a]P and its subsequent effect on anti-oxidant defence system and hippocampal cytomorphometry leading to behavioral changes have not been fully elucidated. We investigated the effect of acute exposure of B[a]P on five days old male Wistar pups administered with single dose of B[a]P (0.2 μg/kg BW) through intracisternal mode. Control group was administered with vehicle i.e., DMSO and a separate group of rats without any treatment was taken as naive group. Behavioral analysis showed anxiolytic-like behavior with significant increase in time spent in open arm in elevated plus maze. Further, significant reduction in fall off time during rotarod test showing B[a]P induced locomotor hyperactivity and impaired motor co-ordination in adolescent rats. B[a]P induced behavioral changes were further associated with altered anti-oxidant defence system involving significant reduction in the total ATPase, Na(+) K(+) ATPase, Mg(2+) ATPase, GR and GPx activity with a significant elevation in the activity of catalase and GST as compared to naive and control groups. Cytomorphometry of hippocampus showed that the number of neurons and glia in B[a]P treated group were significantly reduced as compared to naive and control. Subsequent observation showed that the area and perimeter of hippocampus, hippocampal neurons and neuronal nucleus were significantly reduced in B[a]P treated group as compared to naive and control. The findings of the present study suggest that the alteration in hippocampal cytomorphometry and neuronal population associated with impaired antioxidant signaling and mood in B[a]P treated group could be an outcome of neuromorphological alteration leading to pyknotic cell death or impaired differential migration of neurons during early postnatal brain development. PMID:26946409

  7. Effects of benzo[a]pyrene on mitochondrial and nuclear DNA damage in Atlantic killifish (Fundulus heteroclitus) from a creosote-contaminated and reference site

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Dawoon; Cho, Youngeun [Nicholas School of the Environment, Duke University, Durham, NC 27708 (United States); Collins, Leonard B.; Swenberg, James A. [Center for Environmental Health and Susceptibility, Gillings School of Global Public Health, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599 (United States); Di Giulio, Richard T., E-mail: richd@duke.edu [Nicholas School of the Environment, Duke University, Durham, NC 27708 (United States)

    2009-10-19

    Benzo[a]pyrene (BaP) is a known genotoxicant that affects both mitochondrial and nuclear DNA (mtDNA, nDNA). Here, we examined mtDNA and nDNA damage in the Atlantic killifish (Fundulus heteroclitus) from a highly contaminated Superfund site (Elizabeth River, VA, USA) and from a reference site (King's Creek, VA, USA) that were dosed with 10 mg/kg BaP. Using the long amplicon quantitative PCR technique, we observed similar increases in mitochondrial and nuclear DNA damage in King's Creek fish treated with BaP. Killifish from the Elizabeth River showed high levels of basal nDNA and mtDNA damage compared to fish from the reference site, but the level of damage induced due to BaP treatment was much lower in Elizabeth River killifish compared to King's Creek fish. Laboratory-reared offspring from both populations showed increased BaP-induced damage in mtDNA, relative to nDNA. Similar to the adult experiment, the Elizabeth River larvae had higher levels of basal DNA damage than those from the reference site, but were less impacted by BaP exposure. Measurements of oxidative DNA damage (8-oxo-deoxyguanine by LC-MS/MS) showed no differences among treatment groups, suggesting that the majority of DNA damage is from covalent binding of BaP metabolites to DNA. This study shows for the first time that mitochondria can be an important target of BaP toxicity in fish, indicating that BaP exposures could have important energetic consequences. Results also suggest that multi-generational exposures in the wild may lead to adaptations that dampen DNA damage arising from BaP exposure.

  8. 豆瓣兰的无菌播种与快速繁殖%Asepsis Sowing and in vitro Propagation of Cymbidum serratumr(Schltr)Y.S.Wu et S.C.Chen

    Institute of Scientific and Technical Information of China (English)

    卜朝阳; 何荆洲; 闭志强; 董伟清; 严华兵

    2010-01-01

    @@ 1 植物名称豆瓣兰[Cymbidum serratum(Schltr)Y.S.Wu et S.C.Chen]. 2 材料类别种子. 3 培养条件种子萌发培养基:(1)MS+1 mg·L~(-1)BAP+10%椰子水;(2)改良Kyoto+1 mg·(-1)BAP+10%椰子水;(3)1/3MS+1 mg·L~(-1)BAP+10%椰子水.

  9. Efeito de combinações diferentes de reguladores de crescimento a partir da indução de botões de plântulas de Cattleya walkeriana Gardner (Orchidaceae - DOI: 10.4025/actascibiolsci.v25i1.2106 Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae - DOI: 10.4025/actascibiolsci.v25i1.2106

    Directory of Open Access Journals (Sweden)

    Priscila Vanessa Krapiec

    2003-04-01

    Full Text Available No presente estudo, foram investigadas diferentes combinações e concentrações de auxinas e citocininas para ativar o desenvolvimento de brotos e multiplicar plântulas de Cattleya walkeriana (Orchidaceae, sem estágio intermediário de calos. As plântulas de C. walkeriana foram cultivadas em meio B5, contendo 2% de sacarose, 0,65% de agar e combinações fatoriais das auxinas ácido naftalenoacético (NAA e ácido 3-indolbutírico (IBA, e das citocininas 6-benzilaminopurina (BA e cinetina (KIN, nas concentrações de 0,0; 0,5; 1,0 e 1,5mg/L. O maior número de brotos com folhas desenvolvidas foi formado nas plântulas cultivadas em meio contendo IBA e BA, enquanto o menor número de brotos foi observado na combinação NAA e KIN. Houve maior indução de brotos em meio contendo somente IBA (na ausência de citocininas nas três concentrações. A maior freqüência de indução de brotos usando as combinações de BA e IBA indicaram que essa combinação foi mais efetiva para a multiplicação in vitro de C. walkeriana, usando plântulas como explantesThe present study reports a research with different concentrations and combinations of auxins and cytokinins to activate bud development and to obtain multiplication of Cattleya walkeriana (Orchidaceae without an intermediate callus stage. Seedlings of C. walkeriana were cultured on B5 medium containing 2% sucrose and solidified with 0.65% agar, with factorial combinations of the auxins 1-naphthaleneacetic acid (NAA, 3-indolbutyric acid (IBA, and cytokinins 6-benzylaminopurine (BA and N-(2-furanyl-methyl-1-purine-6 amine (Kinetin at concentrations of 0.0, 0.5, 1.0, and 1.5 mg/L. The highest number of buds with elongated leaves occurred by cultivating seedlings on medium containing different concentrations of IBA and BA, while the lowest number was observed in NAA and KIN combination. IBA alone yielded better results at the three tested concentrations, when compared to the total absence of

  10. HISTORICAL MONITORING OF BIOMARKERS OF EXPOSURE OF BROWN BULLHEAD

    Science.gov (United States)

    Biomarkers of exposure to chemical contamination, benzo(a)pyrene (BAP) and naphthalene (NAPH) type metabolites were measured in brown bullhead from a heavily polycyclic aromatic hydrocarbon (PAH) contaminated section of the Black River, Ohio during and immediately after remedial ...

  11. Some Remarks on the Convex Feasibility Problem and Best Approximation Problem

    Institute of Scientific and Technical Information of China (English)

    Qingzhi Yang; Jinling Zhao

    2008-01-01

    In this paper we investigate several solution algorithms for the convex fea-sibility problem (CFP) and the best approximation problem (BAP) respectively. The algorithms analyzed are already known before, but by adequately reformulating the CFP or the BAP we naturally deduce the general projection method for the CFP from well-known steepest decent method for unconstrained optimization and we also give a natural strategy of updating weight parameters. In the linear case we show the connec-tion of the two projection algorithms for the CFP and the BAP respectively. In addition, we establish the convergence of a method for the BAP under milder assumptions in the linear case. We also show by examples a Bauschke's conjecture is only partially correct.

  12. Multiple shoot-bud formation and plantlet regeneration on Castanea sativa Mill. seeds in culture.

    Science.gov (United States)

    Rodríguez, R

    1982-06-01

    Primordial initiation and development of shoot-buds has been accomplished by using shoots derived from chestnut (Castanea sativa Mill) seedlings cultured with added 6-benzylaminopurine (BAP). Germination of chestnut seeds in the presence of BAP (4 - 40 μM) stimulated varying numbers of shoot-buds in those areas of the main axis that were favorably altered. When excised single shoots from these treated seeds were subcultured on a fresh medium containing BAP (4 - 40 μM) continual shoot production was observed. Bud growth and shoot elongation were stimulated by transferring cultures to a reduced concentration of BAP (2 μM) plus indole-3-butyric acid (IBA 0.4 μM). Plant regeneration occurred in the presence of IBA (0.8 μM) after a preconditioning treatment in which naphthaleneacetic acid (NAA 50 μM) and kinetin (k 2 μM) were applied to the tissue culture shoots for 7 days in light.

  13. Influence of co-existed benzo[a]pyrene and copper on the cellular characteristics of Stenotrophomonas maltophilia during biodegradation and transformation.

    Science.gov (United States)

    Chen, Shuona; Yin, Hua; Ye, Jinshao; Peng, Hui; Liu, Zehua; Dang, Zhi; Chang, Jingjing

    2014-04-01

    Microbial remediation has been proposed as a promising technique to remove pollutions, however, its application has been hindered by the lack of understanding the mechanisms involved in contaminants conversion and the influence of pollutants on cellular characteristics. To address this problem, biodegradation and transformation of BaP-Cu(II) by Stenotrophomonas maltophilia, along with interactions of these pollutants with microbial cells through FCM assay were investigated. The results indicated that BaP and Cu(II) were rapidly removed by S. maltophilia on the 1st d, but only less than 10% BaP was broken down due to temporary store in cells, instead of being decomposed immediately. The key ATP enzymes in cells were then activated by BaP to promote bacteria to further decompose BaP. Stimulation of co-existed contaminants strengthened cell membrane permeability and altered cell structure, but a higher esterase activity and DNA in cells of S. maltophilia were still retained.

  14. Siim Nestor soovitab : Noor, värske ja psühhedeelne hip-hop / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    Keskkooli-noorte hip-hop-kollektiiv BAP presenteerib oma vastilmunud albumit "Who Am I?" 28. veebr. Tallinna Kunstigümnaasiumis, kaastegev äsja albumi "Tabamata Ime" ilmutanud ansambel Luarvik-Luarvik

  15. Degradation of PAHs in soil by Lasiodiplodia theobromae and enhanced benzo[a]pyrene degradation by the addition of Tween-80.

    Science.gov (United States)

    Wang, Cuiping; Liu, Haibin; Li, Jing; Sun, Hongwen

    2014-09-01

    Benzo[a]pyrene (BaP), a five-ring polycyclic aromatic hydrocarbon (PAH), which has carcinogenic potency, is highly recalcitrant and resistant to microbial degradation. A novel fungus, Lasiodiplodia theobromae (L. theobromae), which can degrade BaP as a sole carbon source in liquid, was isolated in our laboratory. To prompt the further application of L. theobromae in remediation of sites polluted by BaP and other PAHs, the present study was targeted toward the removal of BaP and PAHs from soil by L. theobromae. The degradation of BaP by L. theobromae was studied using a soil spiked with 50 mg/kg BaP. L. theobromae could remove 32.1 % of the BaP after 35 days of cultivation. Phenanthrene (PHE) inhibited BaP degradation as a competitive substrate. The tested surfactants enhanced BaP degradation in soil by different extents, and a removal rate of 92.1 % was achieved at a Tween-80 (TW-80) concentration of 5 g/kg. It was revealed that TW-80 could not only enhance BaP bioavailability by increasing its aqueous solubility and decreasing the size of its colloid particles but also increase enzyme secretion from L. theobromae and the population of L. theobromae. Moreover, ergosterol content together with the biomass C indicated the increase in L. theobromae biomass during the BaP biodegradation process in soils. Finally, a soil from a historically PAH-contaminated field at Beijing Coking Plant in China was tested to assess the feasibility of applying L. theobromae in the remediation of polluted sites. The total removal rate of PAHs by L. theobromae was 53.3 %, which is 13.1 % higher than that by Phanerochaete chrysosporium (P. chrysosporium), an effective PAH degrader. The addition of TW-80 to the field soil further enhanced PAH degradation to 73.2 %. Hence, L. theobromae is a promising novel strain to be implemented in the remediation of soil polluted by PAHs. PMID:24878554

  16. The expressions of protooncogenes and CYP1A in lungs of rats exposed to sulfur dioxide and benzo(a)pyrene.

    Science.gov (United States)

    Qin, Guohua; Meng, Ziqiang

    2006-06-01

    Sulfur dioxide (SO2) is a ubiquitous air pollutant, present in low concentrations in the urban air, and in higher concentrations in the working environment. Benzo(a)pyrene (B(a)P), a polycyclic aromatic hydrocarbon, is a ubiquitous environmental contaminant with diverse toxicological effects. To investigate the interactions between SO2 and B(a)P, male Wistar rats were exposed to intratracheally instilled with benzo(a)pyrene (B(a)P; 3 mg) or SO2 (20 ppm) inhalation alone or together. The mRNA of CYP1A1 and 1A2, c-fos, and c-jun and protein levels of c-fos and c-jun were analyzed in lungs using a real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) assay and Western blot analysis, respectively. And 7-ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase (MROD) activities were detected. In lungs of rats exposed to SO2 alone, the gene transcription of CYP1A1 and 1A2, the EROD and MROD activities were decreased. Meanwhile, the mRNA and protein levels of c-jun and c-fos were increased significantly. Exposure to B(a)P alone induced CYP1A1, CYP1A2 mRNA levels, the protein levels of c-jun, and the EROD and MROD activities in lungs. However, exposure to B(a)P plus inhaled SO2 neither increased nor decreased CYP1A1/2 mRNA expressions, EROD, and MROD activities in lungs, versus exposure to B(a)P alone. Nevertheless, exposure to B(a)P plus inhaled SO2 increased the mRNA and protein levels of c-jun and c-fos in lungs compared with lungs exposed to SO2 alone. Accordingly, the SO2-induced decreases of CYP1A1/2 might not influence the metabolic activation of B(a)P. However, when B(a)P and SO2 were given in the combinations, one might postulate that a synergistic effect on the expressions of c-fos and c-jun between SO2 and B(a)P, which might be one of the possible mechanisms of combination effects between B(a)P and the air pollutants.

  17. Propagação in vitro de Sacha inchi In vitro propagation of Sacha inchi

    OpenAIRE

    Stevan Ricardo Bordignon; Glaucia Maria Bovi Ambrosano; Paulo Hercilio Viegas Rodrigues

    2012-01-01

    O objetivo do presente trabalho foi avaliar in vitro a relação auxina:citocinina a fim de obter propágulos nos segmentos distintos do epicótilo e hipocótilo de sementes germinadas in vitro de Sacha inchi (Plukenetia volubilis Linneo). Os segmentos apical (A), mediano (B) e o basal (C) foram introduzidos em meio de cultivo MS, semi sólido (2,0g L-1 Phytagel), suplementado com vitaminas de MS, sacarose (30,0g L-1) e submetidos a três doses da auxina ácido indolbutírico - IBA (0; 0,1; 0,5mg L-1)...

  18. Xeroderma pigmentosum, complementation group D expression in H1299 lung cancer cells following benzo[a]pyrene exposure as well as in head and neck cancer patients.

    Science.gov (United States)

    Lin, Chang-Shen; Chiou, Wen-Yen; Lee, Ka-Wo; Chen, Tzu-Fen; Lin, Yuan-Jen; Huang, Jau-Ling

    2016-01-01

    DNA repair genes play critical roles in response to carcinogen-induced and anticancer therapy-induced DNA damage. Benzo[a]pyrene (BaP), the most carcinogenic polycyclic aromatic hydrocarbon (PAH), is classified as a group 1 carcinogen by International Agency for Research on Cancer. The aims of this study were to (1) evaluate the effects of BaP on DNA repair activity and expression of DNA repair genes in vitro and (2) examine the role of xeroderma pigmentosum, complementation group D (XPD) mRNA expression in human head and neck cancers. Host cell reactivation assay showed that BaP inhibited nucleotide excision repair in H1299 lung cancer cells. DNA repair through the non-homologous end-joining pathway was not affected by BaP. Real-time quantitative reverse-transcription polymerase chain reaction (RT-PCR) and Western blot demonstrated that XPD was downregulated by BaP treatment. BaP exposure did not apparently affect expression of another 11 DNA repair genes. BaP treatment increased the DNA damage marker γ-H2AX and ultraviolet (UV) sensitivity, supporting an impairment of DNA repair in BaP-treated cells. XPD expression was also examined by quantitative RT-PCR in 68 head and neck cancers, and a lower XPD mRNA level was found in smokers' cancer specimens. Importantly, reduced XPD expression was correlated with patient 5-year overall survival rate (35 vs. 56%) and was an independent prognostic factor (hazard ratio: 2.27). Data demonstrated that XPD downregulation was correlated with BaP exposure and human head and neck cancer survival. PMID:26731659

  19. Solasodine accumulation in regenerated plants of Solanum torvum Sw Acúmulo de solasodina em plantas micropropagadas de Solanum torvum Sw

    OpenAIRE

    C. B. Moreira; Lima, S.S. de; M.A Esquibel; A. Sato

    2010-01-01

    A nodal segment culture was developed in order to assess Solanum torvum Sw. regeneration and solasodine levels. The influence of auxins (indoleacetic acid, 1-Naphthaleneacetic acid) and benzyl adenine on S. torvum growth in micropropagation was investigated. A nodal segment culture was initiated with seeds germinated in MS basal medium added of GA3 and grown in different concentrations of IAA, IAA + BAP and NAA + BAP. Sixty-day-old plants from the in vitro culture were collected, frozen and l...

  20. Analysis of fish bile with HPLC — fluorescence to determine environmental exposure to benzo(a)pyrene

    Science.gov (United States)

    Johnston, Eric P.; Baumann, Paul C.

    1989-01-01

    Brown bullhead from the Black River, Ohio, have a high incidence of liver neoplasia which is associated with elevated concentrations of polynuclear aromatic hydrocarbons (PAHs) in the sediment. We evaluated the use of biliary concentrations of benzo(a)pyrene [B(a)P] equivalents as a means for determining PAH exposure. Bile was collected from 16 brown bullheads and 8 common carp taken from each of two Lake Erie tributaries in Ohio, the industrialized Black River and the non-industrialized Old Woman Creek. Hatchery bullhead (n = 8) were used to determine base levels of PAHs. A high performance liquid chromatography (HPLC) — fluorescence technique was used to determine the concentration of B(a)P equivalents in the bile samples. The area of all peaks fluorescing at 380/430 nm was summed to give a single value for B(a)P equivalents in each sample. Concentrations of B(a)P equivalents generally reflected concentrations of PAH in sediment where fish were collected. Bile taken from Black River carp contained the highest concentration of B(a)P equivalents and was significantly different from all other groups. The value obtained for Black River bullhead was also high and was found to be significantly different from hatchery bullhead. B(a)P equivalents varied between carp and bullhead from the same habitat possibly because of differing food habits or metabolic pathways. However, our results indicate that relative levels of B(a)P equivalents in the bile of fish correspond well to B(a)P levels in sediment and may offer a means of determining environmental exposure of fish to the parent compound.

  1. Effect of 2-chloroethylphosphonic acid and vanillin on chlorophyll, protein and RNA synthesis in detached cucumber cotyledons, and chlorophyll degradation in senescing leaf discs of kale

    OpenAIRE

    J. S. Knypl

    2015-01-01

    N6-benzylaminopurine (BAP) and KC1 stimulated growth in detached greening cucumber cotyledons; BAP enhanced RNA synthesis whereas KCl strikingly accelerated leucine-14C incorporation into proteins. Vanillin (0.01 M) inhibited greening and protein synthesis, and nullified the stimulatory effects of KC1. a-chloroethylphosphonic acid (CEPA, 0.01 M) inhibited greening without any effect on protein synthesis when it was applied alone; CEPA decreased RNA synthesis, completely nullified the...

  2. Continuous Variable Solution for Byzantine Agreement

    CERN Document Server

    Neigovzen, R; Neigovzen, Rodion; Sanpera, Anna

    2005-01-01

    We demonstrate that the Byzantine Agreement Problem (BAP) in its weaker version with detectable broadcast, can be solved using continuous variables Gaussian states with Gaussian operations. The protocol uses genuine tripartite symmetric entanglement, but differs from protocols proposed for qutrits or qubits. Contrary to the quantum key distribution (QKD) which is possible with all Gaussian states, for the BAP entanglement is needed, but not all tripartite entangled symmetric states can be used to solve the problem.

  3. Distribution and origin of biologically available phosphorus in the water of the Meiliang Bay in summer

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The investigation and continuous monitoring with an innovative iron oxide embedded cellulose acetate membrane (FeO/CAM) on the concentrations of biologically available phosphorus (BAP) were conducted in the Meiliang Bay of the Taihu Lake during summer in 2004. The results showed that the concentrations of dissolved (FeO-DP), particulate (FeO-PP) and total bioavailable phosphorus (FeO-P) had similar horizontal distribution. The BAP concentrations were the highest in those estuaries in the northern bay. With the decrease of the distance to the estuary or long shore,there was little difference between BAP concentrations in an open lake area. During the observation period, algal blooms occurred in most waters of the northern bay, which was reflected from the high concentrations of chlorophyll a (Chl-a). While they were not highest in the estuarine waters of those major rivers, this is the case for the BAP concentrations. The concentrations of Chl-a had a significantly positive correlation with those of bioavailable phosphorus in the open area of the Meiliang Bay.With the sediment resuspension induced by wind and wave, BAP concentrations increased in a short-term, indicating that the riverine P inputs mainly contribute to the concentrations of BAP in the estuarine water while internal P release was the major source of BAP in the open lake area. In the eutrophic shallow lake, the blooms of alga may cause pH increase and further result in internal P release. The above results showed that the new membrane of FeO/CAM can be used to monitor the concentrations of BAP and provide the scientific justifications for the control strategy of the lake eutrophication.

  4. Shoot Differentiation in Callus Cultures of Datura Innoxia

    DEFF Research Database (Denmark)

    Engvild, Kjeld Christensen

    1973-01-01

    H 5.5, no glycine) in light at 30°C. Callus grew well on any single one of the growth substances NAA (10−5M), 2,4-D (10−6M) or BAP (3 × 10−6M). Growth was less and more erratic on GA or IAA. The callus cultures did not grow significantly better when BAP was combined with one of the auxins or with GA....

  5. Adsorption and partition of benzo(a)pyrene on sediments with different particle sizes from the Yellow River

    Institute of Scientific and Technical Information of China (English)

    XIA Xinghui; MENG Lihong; HU Lijuan

    2007-01-01

    Experiments have been carried out to study the sorption of Benzo(a)pyrene(Bap)on sediment particles from the Yellow River using a batch equilibration technique,Effects of particle size on the adsorption and partition of Bap were investigated with the particle content Of 3 g/L.Several significant results were obtained from the stttdy.(1)Isotherms of Bap could be fired with the dual adsorption-partition model under different particle sizes,and the measured value of the adsorption and partition was in agreement with the theoretical value of the dual adsorption-partition model.(2)When the particle diameter was d≥0.025 mm,the adsorption was predominant in the sorption of Bap,which accounted for 68.7%-82.4% of the sorption.For the particles with the size Of 0.007 mm≤d<0.025 mm,the adsorption was predominant when the equilibrium concentration of Bap was 0-8.87μg/L in the water phase;and the partition was predominant when the equilibrium concentratioh of Bap was higher than 8.87μg/L in the water phase.When the particle diameter was d<0.007 mm,the partition was predominant.(3)On the point of particle size,the contribution of adsorption to sorption followed the order:"d≥0.025 mm">"0.007 mm ≤d<0.025 mm">"d<0.007 mm".(4)The partition coefficients of Bap in solids with different particle sizes were Iinearly correlated with the organic content,and the Koc of Bap was about 1.26×105(L/kg).

  6. DNA Cleavage Promoted by Cu2+ Complex of N,N'-Bis(2-aminoethyl)-2,6-pyridinedicarboxamide

    Institute of Scientific and Technical Information of China (English)

    LI, Ying; SHENG, Xin; SHAO, Ying; LU, Guo-Yuan

    2007-01-01

    The interaction of Cu2+ complex of N,N'-bis(2-aminoethyl)-2,6-pyridinedicarboxamide (BAP) with DNA was studied by agarose gel electrophoresis analysis. The results indicate that the BAP-Cu2+ complex can promote the cleavage of phosphodiester bond of supercoiled DNA at physiological condition, which is 3.2×106 times higher than DNA natural degradation. A hydrolytic cleaving mechanism through the cooperation of copper ions and functional amino groups was proposed.

  7. Biodegradation of benzo[a]pyrene in soil by Mucor sp. SF06 and Bacillus sp. SB02 co-immobilized on vermiculite

    Institute of Scientific and Technical Information of China (English)

    SU Dan; LI Pei-jun; FRANK Stagnitti; XIONG Xian-zhe

    2006-01-01

    Two indigenous microorganisms, Bacillus sp. SB02 and Mucor sp. SF06, capable of degrading polycyclic aromatic hydrocarbons (PAHs) were co-immobilized on vermiculite by physical adsorption and used to degrade benzo[a] pyrane (BaP). The characteristics of BaP degradation by both free and co-immobilized microorganism were then investigated and compared. The removal rate using the immobilized bacterial-ftmgal mixed consortium was higher than that of the freely mobile mixed consortium. 95.3% of BaP was degraded using the co-immobilized system within 42 d, which was remarkably higher than the removal rate of that by the free strains. The optimal amount of inoculated co-immobilized system for BaP degradation was 2%. The immobilized bacterial-ftmgal mixed consortium also showed better water stability than the free strains. Kinetics of BaP biodegradation by co-immobilized SF06 and SB02 were also studied. The results demonstrated that BaP degradation could be well described by a zero-order reaction rate equation when the initial BaP concentration was in the range of 10-200 mg/kg. The scanning electronic microscope (SEM) analysis showed that the co-immobilized microstructure was suitable for the growth of SF06 and SB02. The mass transmission process of co-immobilized system in soil is discussed. The results demonstrate the potential for employing the bacterial-fungal mixed consortium,co-immobilized on vermiculite, for in situ bioremediation of BaP.

  8. Phonological processing in first-degree relatives of individuals with autism: An fMRI study

    OpenAIRE

    Wilson, Lisa B; Tregellas, Jason R.; Slason, Erin; Pasko, Bryce E.; Hepburn, Susan; Rojas, Donald C.

    2012-01-01

    Autism spectrum disorders (ASD) are complex neurodevelopmental disorders. Twin studies have provided heritability estimates as high as 90% for idiopathic ASD. Further evidence for the spectrum’s heritability is provided by the presence of the broad autism phenotype (BAP) in unaffected first-degree relatives. Language ability, specifically phonological processing, is proposed to be a core BAP trait. To date, however, no functional neuroimaging investigations of phonological processing in relat...

  9. Validation of an Analytical Method for Determination of Benzo[a]pyrene Bread using QuEChERS Method by GC-MS.

    Science.gov (United States)

    Eslamizad, Samira; Yazdanpanah, Hassan; Javidnia, Katayon; Sadeghi, Ramezan; Bayat, Mitra; Shahabipour, Sara; Khalighian, Najmeh; Kobarfard, Farzad

    2016-01-01

    A fast and simple modified QuEChERS (quick, easy, cheap, rugged and safe) extraction method based on spiked calibration curves and direct sample introduction was developed for determination of Benzo [a] pyrene (BaP) in bread by gas chromatography-mass spectrometry single quadrupole selected ion monitoring (GC/MS-SQ-SIM). Sample preparation includes: extraction of BaP into acetone followed by cleanup with dispersive solid phase extraction. The use of spiked samples for constructing the calibration curve substantially reduced adverse matrix-related effects. The average recovery of BaP at 6 concentration levels was in range of 95-120%. The method was proved to be reproducible with relative standard deviation less than 14.5% for all of the concentration levels. The limit of detection and limit of quantification were 0.3 ng/g and 0.5 ng/g, respectively. Correlation coefficient of 0.997 was obtained for spiked calibration standards over the concentration range of 0.5-20 ng/g. To the best of our knowledge, this is the first time that a QuEChERS method is used for the analysis of BaP in breads. The developed method was used for determination of BaP in 29 traditional (Sangak) and industrial (Senan) bread samples collected from Tehran in 2014. These results showed that two Sangak samples were contaminated with BaP. Therefore, a comprehensive survey for monitoring of BaP in Sangak bread samples seems to be needed. This is the first report concerning contamination of bread samples with BaP in Iran. PMID:27642317

  10. Variable day/night bias in 24-h non-invasive finger pressure against intrabrachial artery pressure is removed by waveform filtering and level correction.

    Science.gov (United States)

    Westerhof, Berend E; Guelen, Ilja; Parati, Gianfranco; Groppelli, Antonella; van Montfrans, Gert A; Wieling, Wouter; Wesseling, Karel H; Bos, Willem Jan W

    2002-10-01

    BACKGROUND Twenty-four-hour finger arterial pressure (FAP) recordings show a negative bias against intrabrachial artery pressure (BAP) and the bias is greater during the night, thereby overestimating the nocturnal blood pressure dip. We have available a methodology with which to reconstruct BAP from FAP by waveform filtering (transfer function) and generalized level (bias) correction that reduces the bias for short-term blood pressure records. OBJECTIVE To investigate if this methodology also decreases the extra bias during the night, thereby yielding a better estimate of the nocturnal dip. METHODS Twenty-four-hour FAP and BAP blood pressure recordings were simultaneously obtained in eight healthy normotensive volunteers and 14 patients with hypertension (ages 19-60 years), during standardized scheduled activities. The data were analysed off-line, applying the brachial reconstruction technique (reBAP) consisting of a waveform filter and level correction. Simultaneous beats yielded systolic, diastolic and mean pressures that were averaged per 30 min, per day, per night, per activity, over the 24-h period, and for volunteers and patients separately. RESULTS Over the full 24 h, FAP systolic, diastolic and mean values for the total group differed from BAP by +1 +/- 10, -8 +/- 7 and -10 +/- 8 mmHg (mean +/- SD), respectively. Similarly, reBAPs differed by +1 +/- 11, -2 +/- 7 and -2 +/- 7 mmHg. BAPs dipped by 20 +/- 8, 13 +/- 6 and 15 +/- 6 mmHg, respectively, during the night. These dips were overestimated by +8, +4 and +4 mmHg by FAP, but not by reBAP: -1, +1 and +1 mmHg. The volunteer and the patient groups showed slight differences in results, but these were not statistically significant. CONCLUSIONS The generalized reconstruction technique to obtain near-brachial pressure from non-invasive FAP almost completely removed bias over the full 24-h day-night period and improved tracking of diurnal changes for all three blood pressure values. PMID:12359976

  11. Chronic exposure to low benzo[a]pyrene level causes neurodegenerative disease-like syndromes in zebrafish (Danio rerio).

    Science.gov (United States)

    Gao, Dongxu; Wu, Meifang; Wang, Chonggang; Wang, Yuanchuan; Zuo, Zhenghong

    2015-10-01

    Previous epidemiological and animal studies report that exposure to environmental pollutant exposure links to neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Benzo[a]pyrene (BaP), a neurotoxic polycyclic aromatic hydrocarbon, has been increasingly released into the environment during recent decades. So far, the role of BaP on the development of neurodegenerative diseases remaind unclear. This study aimed to determine whether chronic exposure to low dose BaP would cause neurodegenerative disease-like syndromes in zebrafish (Danio rerio). We exposed zebrafish, from early embryogenesis to adults, to environmentally relevant concentrations of BaP for 230 days. Our results indicated that BaP decreased the brain weight to body weight ratio, locomotor activity and cognitive ability; induced the loss of dopaminergic neurons; and resulted in neurodegeneration. In addition, obvious cell apoptosis in the brain was found. Furthermore, the neurotransmitter levels of dopamine and 3,4-dihydroxyphenylacetic acid, the mRNA levels of the genes encoding dopamine transporter, Parkinson protein 7, phosphatase and tensin-induced putative kinase 1, ubiquitin carboxy-terminal hydrolase L1, leucine-rich repeat serine/threonine kinase 2, amyloid precursor protein b, presenilin 1 and presenilin 2 were significantly down-regulated by BaP exposure. These findings suggest that chronic exposure to low dose BaP could cause the behavioral, neuropathological, neurochemical, and genetic features of neurodegenerative diseases. This study provides clues that BaP may constitute an important environmental risk factor for neurodegenerative diseases in humans. PMID:26349946

  12. System for managing nomenclatures Sistema para la gestión de nomencladores

    OpenAIRE

    Renán Vázquez Moreno; José Mojena Alpizar

    2012-01-01

    From the need to manage common information and relatively constant over time creates the Nomenclature System Evaluation and Planning System of Medical Supplies (wings BAP), in order to centralize the management of nomenclatures. This module was deficient in its operation: there were no Web services that allow access to information from other systems and nomenclatures presented total dependence wings BAP system, so the task was to develop a system that would eliminate the existing shortcomings...

  13. Prosodic knowledge affects the recognition of newly acquired words

    OpenAIRE

    Shatzman, K.; McQueen, J

    2006-01-01

    An eye-tracking study examined the involvement of prosodic knowledge—specifically, the knowledge that monosyllabic words tend to have longer durations than the first syllables of polysyllabic words—in the recognition of newly learned words. Participants learned new spoken words (by associating them to novel shapes): bisyllables and onset-embedded monosyllabic competitors (e.g., baptoe and bap). In the learning phase, the duration of the ambiguous sequence (e.g., bap) was held constant. In the...

  14. Models and Heuristics for the Tactical Berth Allocation Problem with Quay-Crane Assignment and Transshipment Costs

    OpenAIRE

    Giallombardo, Giovanni; Moccia, Luigi; Salani, Matteo; Vacca, Ilaria

    2009-01-01

    In the context of international sea-freight container transport, we study an integrated decision problem arising in container terminal management. We consider the integration of the Berth Allocation Problem (BAP), which consists of assigning and scheduling incoming ships to berthing positions, and the Quay Crane Assignment Problem (QCAP), which assigns to incoming ships a certain QC profile (i.e. number of quay cranes per working shift). BAP and QCAP are strictly correlated, since the QC prof...

  15. Effect of ageing on benzo[a]pyrene extractability in contrasting soils

    Energy Technology Data Exchange (ETDEWEB)

    Duan, Luchun [CERAR-Centre for Environmental Risk Assessment and Remediation and Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC CARE), Building X, University of South Australia, Mawson Lakes, SA 5095 (Australia); Naidu, Ravi, E-mail: Ravi.Naidu@newcastle.edu.au [CERAR-Centre for Environmental Risk Assessment and Remediation and Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC CARE), Building X, University of South Australia, Mawson Lakes, SA 5095 (Australia); Liu, Yanju; Palanisami, Thavamani; Dong, Zhaomin; Mallavarapu, Megharaj [CERAR-Centre for Environmental Risk Assessment and Remediation and Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC CARE), Building X, University of South Australia, Mawson Lakes, SA 5095 (Australia); Semple, Kirk T. [Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ (United Kingdom)

    2015-10-15

    Highlights: • In vitro assessment of B[a]P in contaminated soils using 4 different methods. • An exponential kinetic model fits well with the extractability data. • Fitting parameter and {sup 14}C residue correlates with key soil properties. • Fractionation of B[a]P was obtained based on extractability by extractants. - Abstract: Changes in benzo[a]pyrene (B[a]P) extractability over 160 days ageing in four contrasting soils varying in organic matter content and clay mineralogy were investigated using dichloromethane: acetone 1:1 (DCM/Ace), 60 mM hydroxypropyl-β-cyclodextrin (HPCD) solution, 1-butanol (BuOH) and Milli-Q water. The B[a]P extractability by the four methods decreased with ageing and a first-order exponential model could be used to describe the kinetics of release. Correlation of the kinetic rate constant with major soil properties showed a significant effect of clay and sand contents and pore volume fraction (<6 nm) on sequestration of the desorbable fraction (by HPCD) and the water-extractable fraction. Analysis of {sup 14}C-B[a]P in soils after ageing showed a limited loss of B[a]P via degradation. Fractionation of B[a]P pools associated with the soil matrix was analysed according to extractability of B[a]P by the different extraction methods. A summary of the different fractions is proposed for the illustration of the effect of ageing on different B[a]P-bound fractions in soils. This study provides a better understanding of the B[a]P ageing process associated with different fractions and also emphasises the extraction capacity of the different methods employed.

  16. ANATOMICAL STUDIES OF IN VITRO ORGANOGENESIS INDUCED IN LEAF-DERIVED EXPLANTS OF PASSIONFRUIT ESTUDOS ANATÔMICOS DA ORGANOGÊNESE IN VITRO INDUZIDA EM EXPLANTES DE FOLHA DE MARACUJÁ

    OpenAIRE

    BEATRIZ APPEZZATO DA GLORIA; MARIA LUCIA CARNEIRO VIEIRA; MARCELO CARNIER DORNELAS

    1999-01-01

    With the aim of studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropro...

  17. Shoot Regeneration from Leaf Explants of Withania somnifera (L. Dunal

    Directory of Open Access Journals (Sweden)

    Aruna Girish JOSHI

    2010-03-01

    Full Text Available Regeneration from leaf explants of Withania somnifera (L. for mass propagation was studied on Murashige and Skoog�s medium supplemented with Kinetin (Kn and 6-benzylaminopurine (BAP alone or in combination. Shoot buds were induced from the midrib on the abaxial side in presence of Kn and BAP (4 �M. These shoot buds developed into shoots on the same medium. Rooting of these shoots was achieved in 0.5 �M of IBA.

  18. Alterations in benzo(A)pyrene metabolism and in vivo binding to hepatic DNA in rats red diets containing menhaden oil

    Energy Technology Data Exchange (ETDEWEB)

    Wade, A.E.; Dharwadkar, S.

    1987-01-01

    Polyunsaturated fatty acids of the omega-6 type have been shown to support the mixed function oxidases (MFO) responsible for carcinogen activation and to promote tumorigenesis in laboratory animals. The omega-3 fatty acids contained in menhaden oil (MO) have been shown to enhance MFO activity and increase the binding of Benzo(a)pyrene (B(a)P) metabolites to calf thymus DNA in an in vitro microsomal system. Rats fed two levels of MO (0.5% and 20%) for 11 days received a single i.p. dose of (/sup 3/H)B(a)P (5 m Ci/kg) dissolved in DMSO. At selected time intervals thereafter rats were killed, blood withdrawn, livers removed and DNA extracted. Hepatic microsomes were recovered from control rats on each diet at the time of B(a)P administration to assess MFO activities. Binding of B(a)P to DNA was higher in rats fed the 20% MO diet suggesting an increased rate of B(a)P activation. Blood levels of B(a)P were elevated at 16 and 24 hours post B(a)P, however no differences in urine concentrations were observed. Elevations in concentration of cytochrome P-450, ethoxycoumarin dealkylase, and glutathione S-transferase suggest that omega-3 fatty acids of menhaden fish oil support MFO related reactions not unlike the omega-6 fatty acids.

  19. Modulatory effects of catechin hydrate against genotoxicity, oxidative stress, inflammation and apoptosis induced by benzo(a)pyrene in mice.

    Science.gov (United States)

    Shahid, Ayaz; Ali, Rashid; Ali, Nemat; Hasan, Syed Kazim; Bernwal, Preeti; Afzal, Shekh Mohammad; Vafa, Abul; Sultana, Sarwat

    2016-06-01

    Benzo(a)pyrene [B(a)P], a polycyclic aromatic hydrocarbon (PAH) is a strong mutagen and potent carcinogen. The aim of the present study was to investigate the efficacy of catechin hydrate against B(a)P induced genotoxicity, oxidative stress, inflammation, apoptosis and to explore its underlying molecular mechanisms in the lungs of Swiss albino mice. Administration of B(a)P (125 mg/kg b. wt., p. o.) increased the activities of toxicity markers such as LPO, LDH and B(a)P metabolizing enzymes [NADPH-cytochrome P450 reductase (CYPOR) and microsomal epoxide hydrolase (mEH)] with subsequent decrease in the activities of tissue anti-oxidant armory (SOD, CAT, GPx, GR, GST, QR and GSH). It also caused DNA damage and activation of apoptotic and inflammatory pathway by upregulation of TNF-α, IL-6, NF-kB, COX-2, p53, bax, caspase-3 and down regulating Bcl-2. However, pre-treatment with catechin at a dose of 20 and 40 mg/kg significantly decreased LDH, LPO, B(a)P metabolizing enzymes and increased anti-oxidant armory as well as regulated apoptosis and inflammation in lungs. Histological results also supported the protective effects of catechin. The findings of the present studies suggested that catechin as an effective natural product attenuates B(a)P induced lung toxicity. PMID:27020533

  20. The transcriptional coactivator SAYP is a trithorax group signature subunit of the PBAP chromatin remodeling complex.

    Science.gov (United States)

    Chalkley, Gillian E; Moshkin, Yuri M; Langenberg, Karin; Bezstarosti, Karel; Blastyak, Andras; Gyurkovics, Henrik; Demmers, Jeroen A A; Verrijzer, C Peter

    2008-05-01

    SWI/SNF ATP-dependent chromatin remodeling complexes (remodelers) perform critical functions in eukaryotic gene expression control. BAP and PBAP are the fly representatives of the two evolutionarily conserved major subclasses of SWI/SNF remodelers. Both complexes share seven core subunits, including the Brahma ATPase, but differ in a few signature subunits; POLYBROMO and BAP170 specify PBAP, whereas OSA defines BAP. Here, we show that the transcriptional coactivator and PHD finger protein SAYP is a novel PBAP subunit. Biochemical analysis established that SAYP is tightly associated with PBAP but absent from BAP. SAYP, POLYBROMO, and BAP170 display an intimately overlapping distribution on larval salivary gland polytene chromosomes. Genome-wide expression analysis revealed that SAYP is critical for PBAP-dependent transcription. SAYP is required for normal development and interacts genetically with core- and PBAP-selective subunits. Genetic analysis suggested that, like BAP, PBAP also counteracts Polycomb silencing. SAYP appears to be a key architectural component required for the integrity and association of the PBAP-specific module. We conclude that SAYP is a signature subunit that plays a major role in the functional specificity of the PBAP holoenzyme.

  1. Detection of benzo[a]pyrene-induced immunotoxicity in orange spotted grouper (Epinephelus coioides).

    Science.gov (United States)

    Khaniyan, Maryam; Salamat, Negin; Safahieh, Alireza; Movahedinia, Abdolali

    2016-03-01

    This study aimed to investigate the effects of benzo[a]pyrene (BaP) on immune status of orange spotted grouper (Epinephelus coioides). Fish were injected with 2, 20 and 35 mg/kg-bw of BaP and were kept under laboratory conditions for 14 days. Blood samples were taken at days 1, 4, 7, and 14 and changes in total WBC and RBC, phagocytosis, lysozyme activity, lysosomal membrane stability, immunoglobulin M (IgM) level and antibacterial activity were evaluated. Also BaP bioaccumulation in fish muscle was measured. BaP concentration in the muscle of treated fish reached a maximum level after 4 days (P < 0.05). Exposure of fish to BaP resulted in a significant decrease of total RBC and WBC, lysozyme activity, lysosomal membrane stability, IgM level and antibacterial activity after 4 days and phagocytosis after 7 days of the experiment (P < 0.05). Totally, the results revealed BaP ability to suppress the fish immune function.

  2. Effects of quantity, quality, and contact time of dissolved organic matter on bioconcentration of benzo[a]pyrene in the nematode Caenorhabditis elegans

    Energy Technology Data Exchange (ETDEWEB)

    Haitzer, M.; Hoess, S. [Ludwig Maximilians Univ. Muenchen (Germany). Zoologisches Inst.]|[Inst. fuer Gewaesseroekologie und Binnenfischerei, Berlin (Germany); Burnison, B.K. [Environment Canada, Burlington, Ontario (Canada). National Water Research Inst.; Traunspurger, W. [Ludwig Maximilians Univ. Muenchen (Germany). Zoologisches Inst.; Steinberg, C.E.W. [Inst. fuer Gewaesseroekologie und Binnenfischerei, Berlin (Germany)

    1999-03-01

    Quantity and quality of dissolved organic matter (DOM) and the time allowed for DOM to interact with organic contaminants can influence their bioavailability. The authors studied the effect of natural aquatic DOM that had been in contact with benzo[a]pyrene (B[a]P) for 1 to 12 d on the bioconcentration of B[a]P in the nematode Caenorhabditis elegans. Dissolved organic matter quality and quantity was varied by using DOM from three different sources, each in three different concentrations. A model, based on the assumption that only freely dissolved B[a]P is bioavailable, was employed to estimate biologically determined partition coefficients [K{sub p}(biol.)]. Expressing the data for each combination of DOM source and contact time in a single K{sub p} (biol.) value allowed a direct comparison of the effects of different DOM qualities and contact times. The results show that the effect of DOM from a specific source was dependent on DOM quantity, but they also observed a distinct effect of DOM quality (represented by different sampling locations) on the bioconcentration of B[a]P. Contact time had no significant influence for the effects of two DOM sources on the bioconcentration of B[a]P. However, the third DOM source was significantly more effective with increased contact time, leading to lower B[a]P bioconcentration in the nematodes.

  3. Is the smokers exposure to environmental tobacco smoke negligible?

    Directory of Open Access Journals (Sweden)

    Valerio Federico

    2010-01-01

    Full Text Available Abstract Background Very few studies have evaluated the adverse effect of passive smoking exposure among active smokers, probably due to the unproven assumption that the dose of toxic compounds that a smoker inhales by passive smoke is negligible compared to the dose inhaled by active smoke. Methods In a controlled situation of indoor active smoking, we compared daily benzo(apyrene (BaP dose, estimated to be inhaled by smokers due to the mainstream (MS of cigarettes they have smoked, to the measured environmental tobacco smoke (ETS they inhaled in an indoor environment. For this aim, we re-examined our previous study on daily personal exposure to BaP of thirty newsagents, according to their smoking habits. Results Daily BaP dose due to indoor environmental contamination measured inside newsstands (traffic emission and ETS produced by smoker newsagents was linearly correlated (p = 0.001 R2 = 0.62 with estimated BaP dose from MS of daily smoked cigarettes. In smoker subjects, the percentage of BaP daily dose due to ETS, in comparison to mainstream dose due to smoked cigarettes, was estimated with 95% confidence interval, between 14.6% and 23% for full flavour cigarettes and between 21% and 34% for full flavour light cigarettes. Conclusions During indoor smoking, ETS contribution to total BaP dose of the same smoker, may be not negligible. Therefore both active and passive smoking exposures should be considered in studies about health of active smokers.

  4. PAH in Barbecued Meat from Restaurants and by Home-Grilling in Denmark

    DEFF Research Database (Denmark)

    Duedahl-Olesen, Lene; Meinert, L.; Aaslyng, M.;

    2011-01-01

    [a]pyrene (BaP), benzo[a]anthracene, benzo[b]fluoranthene and chrysene). PAHs were extracted by Pressurized Liquid Extraction followed by clean-up by GPC (Bio Beads, S-X3) and SPE (500mg, Silica) and finally analyzed by GC-MS. Home-grilled beef were found to contain highest concentrations of BaP (up to 24 µg....../kg) whereas commercially barbecued hamburgers were found to contain BaP up to 13.7 µg/kg. Chicken was found to contain the lowest BaP concentrations (0-0.1µg/kg). The highest concentration of BaP for chicken was 3.1 µg/kg detected in a commercially barbecued marinated chicken with skin. The sum of PAH 4...... ranged from below the detection limit to the highest concentration for a home-grilled beef (64 µg/kg). Worst case estimates revealed a margin of exposure (MOE) of approximately 18,000 for both BaP and the sum of PAH 4 for grilled meat only. The European Food Safety Authority (EFSA) has previously...

  5. Investigation of nutrient sensing in the yeast Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Eckert-Boulet, Nadine

    2006-01-01

    -citrulline kan ikke optages af disse stammer, da de mangler GAP1 (General Amino acid Permease). Det blev bekræftet, at AAP generne TAT1, BAP2, BAP3 og PTR2 er under kontrol af aminosyre induktionsvejen, mens AGP2 blev identificeret som et nyt gen under kontrol af denne mekanisme. Den globale...... for at aminosyre induktion kan finde sted. Hel-genom transkriptionsanalyser af en vildtype stamme og en grr1 stamme med og uden L-citrullin blev udført. Dataanalyse viste at aminosyre induktion af AAP generne AGP1, BAP2, BAP3, DIP5, TAT1, og GNP1 er helt afhængig af tilstedeværelsen af GRR1. Sammenligningen af de....... RTS1 blev slået ud, og dette medførte konstitutiv aktivering af AGP1 og BAP2 promotorer. Dette var afhængigt af SSY1, PTR3, SSY5, GRR1 eller STP1, STP2 og deres homolog STP3. Den forøgede transkription fra AGP1 og BAP2 promotorer i rts1 celler ser ud til at skyldes forøget processering af Stp1p. Med...

  6. Effects of polycyclic aromatic hydrocarbons (PAHs) on an aquatic ecosystem: acute toxicity and community-level toxic impact tests of benzo[a]pyrene using lake zooplankton community.

    Science.gov (United States)

    Ikenaka, Yoshinori; Sakamoto, Masaki; Nagata, Takamaru; Takahashi, Hirokazu; Miyabara, Yuichi; Hanazato, Takayuki; Ishizuka, Mayumi; Isobe, Tomohiko; Kim, Jun-Woo; Chang, Kwang-Hyeon

    2013-02-01

    We estimated acute toxicity of benzo[a]pyrene (B[a]P) using two cladoceran species, Ceriodaphnia reticulata and Daphnia magna, and also analyzed its impact on zooplankton community throughout an exposure experiment using small-scale mesocosms. LC(50) of B[a]P for C. reticulata and D. magna was 4.3 and 4.7 µg/l, respectively. However, individuals fed with Chlorella showed higher LC(50), 6.1 µg/l for C. reticulata and 8.0 µg/l for D. magna. In the exposure experiment, we examined the impact of B[a]P on zooplankton community using conceivable concentrations in the environment (5 and 10 µg/l) using typical zooplankton community in eutrophicated systems. Despite the residence time of B[a]P in the water column was short as impacts, suppressing cladoceran populations and inducing the dominance of rotifers particularly under high concentration (10 µg/l). Results have suggested that, even such short duration of B[a]P in the water body can have impact on zooplankton abundance and community structure. Since B[a]P easily precipitate to the bottom and rapidly disappears from the water body, careful monitoring and further assessment of the potential toxicity of polycyclic aromatic hydrocarbons are necessary.

  7. Theory of Mind Indexes the Broader Autism Phenotype in Siblings of Children with Autism at School Age.

    Science.gov (United States)

    Tsang, Tawny; Gillespie-Lynch, Kristen; Hutman, Ted

    2016-01-01

    Subclinical variants of the social-communicative challenges and rigidity that define autism spectrum disorder (ASD) are known as the broader autism phenotype (BAP). The BAP has been conceptualized categorically (as specific to a subset of relatives of individuals with ASD) and dimensionally (as continuously distributed within the general population). The current study examined the compatibility of these two approaches by assessing associations among autism symptoms and social-communicative skills in young school-age children with ASD, children who have a sibling with ASD, and children without a sibling with ASD. Autism symptoms were associated with reduced Theory of Mind (ToM), adaptive skills, cognitive empathy, and language skills across the full sample. Reduced ToM was a core aspect of the BAP in the current sample regardless of whether the BAP was defined categorically (in terms of siblings of children with ASD who exhibited atypical developmental) or dimensionally (in terms of associations with autism symptoms across the entire sample). Early language skills predicted school-age ToM. Findings support the compatibility of categorical and dimensional approaches to the BAP, highlight reduced ToM as a core aspect of the school-age BAP, and suggest that narrative-based approaches to promoting ToM may be beneficial for siblings of children with ASD. PMID:26881074

  8. Theory of Mind Indexes the Broader Autism Phenotype in Siblings of Children with Autism at School Age

    Science.gov (United States)

    Tsang, Tawny; Gillespie-Lynch, Kristen; Hutman, Ted

    2016-01-01

    Subclinical variants of the social-communicative challenges and rigidity that define autism spectrum disorder (ASD) are known as the broader autism phenotype (BAP). The BAP has been conceptualized categorically (as specific to a subset of relatives of individuals with ASD) and dimensionally (as continuously distributed within the general population). The current study examined the compatibility of these two approaches by assessing associations among autism symptoms and social-communicative skills in young school-age children with ASD, children who have a sibling with ASD, and children without a sibling with ASD. Autism symptoms were associated with reduced Theory of Mind (ToM), adaptive skills, cognitive empathy, and language skills across the full sample. Reduced ToM was a core aspect of the BAP in the current sample regardless of whether the BAP was defined categorically (in terms of siblings of children with ASD who exhibited atypical developmental) or dimensionally (in terms of associations with autism symptoms across the entire sample). Early language skills predicted school-age ToM. Findings support the compatibility of categorical and dimensional approaches to the BAP, highlight reduced ToM as a core aspect of the school-age BAP, and suggest that narrative-based approaches to promoting ToM may be beneficial for siblings of children with ASD. PMID:26881074

  9. Preferential orientation of biological apatite in normal and osteoporotic human vertebral trabeculae

    Science.gov (United States)

    Miyabe, S.; Ishimoto, T.; Nakano, T.

    2009-05-01

    The preferential orientation of biological apatite (BAp) is a possible bone quality parameter for the comparison of the bone mechanical property. The preferential BAp orientation undergoes sensitive changes according to the change in the in vivo stress distribution, bone turnover rate etc., resulting in a variation of bone function. Osteoporosis is a metabolic bone disease characterized by reduced bone mass and deterioration of bone microstructure. The effect of osteoporosis on the preferential BAp orientation is however unknown. In this study, a microbeam-X-ray diffraction (μXRD) study was carried out on a trabecula extracted from osteoporotic and normal human vertebral bones and the degree of orientation for the BAp c-axis along its craniocaudal axis was analysed based on our previous report. A micro-computed tomography (μCT) measurement was also performed to analyze trabecular density and structure. In osteoporotic human vertebra, the trabecular number is markedly lower than that in normal vertebra. To sustain increased stress because of bone loss, the primary trabeculae, which are aligned parallel to the craniocaudal axis, tend to selectively remain while the secondary trabeculae, which are perpendicular to the craniocaudal axis, mostly disappear. Moreover, the primary trabecula from osteoporotic vertebra showed a significantly higher degree of BAp preferential orientation than the normal bone. This suggests that the remaining primary trabecula in osteoporotic vertebra is further reinforced by an increase in applied stress in vivo by enhancing the preferred BAp c-axis orientation along the trabecular direction.

  10. Measurement of human CYP1A2 induction by inhalation exposure to benzo(a)pyrene based on in vivo isotope breath method.

    Science.gov (United States)

    Duan, Xiaoli; Shen, Guofeng; Yang, Hongbiao; Lambert, George; Wei, Fusheng; Zhang, Junfeng Jim

    2016-01-01

    Cytochrome P450 1A2 (CYP1A2) is an enzyme involved in the metabolic activation of certain carcinogens, and inducible by toxic substrates. To date, few studies have investigated in vivo CYP1A2 induction in humans and its relationship to polycylic aromatic hydrocarbons (PAHs) like benzo(a)pyrene (BaP). Non-smoking healthy male coke-oven workers (n = 30) were recruited as 'exposure' group, and non-smoking healthy office workers in the same city (n = 10) were selected as 'control' group, to test whether high inhalation exposure to PAHs can induce CYP1A2 activity in human livers. Significantly higher inhalation exposure of PAHs were found among the exposure group compared to the control. Inhalation BaP exposure concentration in the exposure group was more than 30 times higher than the control group (p < 0.001). However, the exposure group did not exhale significant higher levels of (13)CO2/(12)CO2 in breath samples (p = 0.81), and no significant relationship was found between the inhaled BaP concentration and the (13)CO2/(12)CO2 ratio (p = 0.91). A significant association was found between the (13)CO2/(12)CO2 exhalation and dietary BaP intake level. Hepatic CYP1A2 activity/induction level was not effected by inhaled BaP but was altered by ingestion of BaP. PMID:26552516

  11. Benzo[a]pyrene treatment leads to changes in nuclear protein expression and alternative splicing

    Energy Technology Data Exchange (ETDEWEB)

    Yan Chunlan; Wu Wei [Department of Toxicology, Zhejiang University School of Public Health, 388 Yu-Hang-Tang Road, Hangzhou, Zhejiang 310058 (China); Li Haiyan [Department of Toxicology, Zhejiang University School of Public Health, 388 Yu-Hang-Tang Road, Hangzhou, Zhejiang 310058 (China); Huzhou Maternity and Child Care Hospital, Huzhou, Zhejiang 313000 (China); Zhang Guanglin [Department of Toxicology, Zhejiang University School of Public Health, 388 Yu-Hang-Tang Road, Hangzhou, Zhejiang 310058 (China); Duerksen-Hughes, Penelope J. [Department of Basic Sciences, Loma Linda University School of Medicine, Loma Linda, CA 92354 (United States); Zhu Xinqiang, E-mail: zhuxq@zju.edu.cn [Department of Toxicology, Zhejiang University School of Public Health, 388 Yu-Hang-Tang Road, Hangzhou, Zhejiang 310058 (China); Yang Jun, E-mail: gastate@zju.edu.cn [Department of Toxicology, Zhejiang University School of Public Health, 388 Yu-Hang-Tang Road, Hangzhou, Zhejiang 310058 (China); Zhejiang-California International Nanosystems Institute, Hangzhou, Zhejiang 310029 (China)

    2010-04-01

    Benzo[a]pyrene (BaP) is a potent pro-carcinogen generated from the combustion of fossil fuel and cigarette smoke. Previously, using a proteomic approach, we have shown that BaP can induce changes in the expression of many cellular proteins, including transcription regulators. In the present study, using a similar approach, we examined the nuclear protein response to BaP in HeLa cells and found that BaP treatment caused expression changes in many nuclear proteins. Twenty-four of these proteins were successfully identified, several of which are involved in the alternative splicing of mRNA, DNA replication, recombination, and repair. The changed expression levels were further confirmed by immunoblot analysis using specific antibodies for two proteins, Lamin A and mitotic checkpoint protein Bub3. The nuclear localization of these two proteins was also confirmed by confocal microscopy. To determine whether alternative splicing was activated following BaP treatment, we examined Fas and CD44, two genes previously shown to be targets of alternative splicing in respond to DNA damage. While no significant activation of alternative splicing was observed for Fas, CD44 splicing variants were found after BaP treatment. Together, these data show that DNA damage induces dramatic changes in nuclear protein expression, and that alternative splicing might be involved in the cellular response to DNA damage.

  12. Theory of Mind Indexes the Broader Autism Phenotype in Siblings of Children with Autism at School Age

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    Tawny Tsang

    2016-01-01

    Full Text Available Subclinical variants of the social-communicative challenges and rigidity that define autism spectrum disorder (ASD are known as the broader autism phenotype (BAP. The BAP has been conceptualized categorically (as specific to a subset of relatives of individuals with ASD and dimensionally (as continuously distributed within the general population. The current study examined the compatibility of these two approaches by assessing associations among autism symptoms and social-communicative skills in young school-age children with ASD, children who have a sibling with ASD, and children without a sibling with ASD. Autism symptoms were associated with reduced Theory of Mind (ToM, adaptive skills, cognitive empathy, and language skills across the full sample. Reduced ToM was a core aspect of the BAP in the current sample regardless of whether the BAP was defined categorically (in terms of siblings of children with ASD who exhibited atypical developmental or dimensionally (in terms of associations with autism symptoms across the entire sample. Early language skills predicted school-age ToM. Findings support the compatibility of categorical and dimensional approaches to the BAP, highlight reduced ToM as a core aspect of the school-age BAP, and suggest that narrative-based approaches to promoting ToM may be beneficial for siblings of children with ASD.

  13. Substancias con actividad citoquinínica estimulan la brotación de yemas en tubérculos de papa Substâncias com atividade citocinínica estimulam a brotação de gemas em tubérculos de batata Cytokinin-like substences activity promotes shoot sprouting in potato tubers

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    Manuel García-Flórez

    2009-09-01

    ção coordenada de vários fitorreguladores. A presença de citocininas em S. phureja Juz. et Buk. var. Criolla Colombia e em S. tuberosum L. variedades ICA-Única e Tuquerreña foi determinada em diferentes estádios do desenvolvimento do tubérculo: 00 - tubérculo em estádio de dormência; 03 - tubérculo no final da dormência e brotos com 2 a 3 mm de comprimento; e 40 - início da tuberização, espessamento das extremidades dos estolões ao dobro do diâmetro inicial. As citocininas foram extraídas por cromatografia de camada delgada e sua detecção e quantificação observadas por meio do bioensaio da massa cotiledonar de rabanete (Raphanus sativus. Na espécie precoce S. phureja Juz. et Buk. var. Criolla Colombia a presença de citocininas foi detectada em quantidades significativas nos estádios 00 e 03. Por outro lado, nas variedades de S. tuberosum L. as citocininas foram significativamente maiores no estádio 03 para 'ICA-Única' (variedade precoce, e a sua presença não foi detectada na var. Tuquerreña (variedade tardia. Demonstrou-se a relação direta entre a concentração de citocininas e a precocidade das variedades analisadas durante a tuberização: as citocininas estiveram presentes nas variedades precoces e ausentes na tardia.Before sprouting, potato tubers pass through a period of dormancy which has an specific time for each variety. When the conditions are suitable for tuberset, stolon elongation stops and swelling starts between two last nodes of each stolon. Many factors are involved in this process, but an important and coordinated action between hormones is proposed. Cytokinins levels in both S. tuberosum L. varieties ICA-Única and Tuquerreña and S. phureja Juz. et Buk. var. Criolla Colombia were quantified during different stages of tuber development: 00 - potato tuber in stage of dormancy; 03 - tubers in break dormancy with buds between 2 to 3 mm of length; and 40 - initiation of tuber formation; swelling and duplicating initial diameter

  14. Callus induction in leaf segments of Croton urucurana Baill Indução de calos em segmentos foliares de sangra d'água (Croton urucurana Baill

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    Ednabel Caracas Lima

    2008-02-01

    Full Text Available Croton urucurana Baill., a species belonging to the family Euphorbiaceae, can be useful in the recovery of degraded riparian areas. In the traditional medicine, it is widely used as cicatrizant and in the treatment of rheumatism. However, its seeds present dormancy and low viability, making the propagation of this species a challenge. With the objective of establishing an alternative route for the propagation, the effect of different concentrations of 2,4-D (2,4- dichlorophenoxyacetic acid with BAP (6-benzylaminopurine or TDZ (thidiazuron and of NAA (1-naphthaleneacetic acid with BAP and their possible combinations were evaluated for callus induction in leaf segments. Callus fresh mass was evaluated forty-five days after inoculation. The isolated use of BAP and the combination of NAA with BAP did not promote calogenesis in leaf segments. Even though the combination of 2,4-D with BAP or TDZ had induced the formation of callus, it was the isolated use of 2,4-D that provided the highest callus fresh mass.A sangra d'água, espécie pertencente à família Euphorbiaceae, apresenta potencial na recuperação de matas ciliares degradadas e é extensamente utilizada na medicina tradicional, como cicatrizante e no tratamento de reumatismos. Entretanto, suas sementes apresentam dormência e baixa viabilidade, dificultando a propagação desta espécie. Avaliou-se o efeito de diferentes concentrações de 2,4-D e BAP ou TDZ e de ANA e BAP, com suas possíveis combinações, na indução de calos em segmentos foliares de sangra d'água. Quarenta e cinco dias após a inoculação, foi avaliado o peso fresco dos calos. O uso de BAP isoladamente e a combinação entre ANA e BAP não promoveram calogênese em segmentos foliares de sangra d'água. Embora a combinação de 2,4-D e BAP ou TDZ tenha induzido a formação de calos, o uso isolado de 2,4-D proporcionou o maior peso fresco destes.

  15. Influence of cell cycle on responses of MCF-7 cells to benzo[a]pyrene

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    Giddings Ian

    2011-06-01

    Full Text Available Abstract Background Benzo[a]pyrene (BaP is a widespread environmental genotoxic carcinogen that damages DNA by forming adducts. This damage along with activation of the aryl hydrocarbon receptor (AHR induces complex transcriptional responses in cells. To investigate whether human cells are more susceptible to BaP in a particular phase of the cell cycle, synchronised breast carcinoma MCF-7 cells were exposed to BaP. Cell cycle progression was analysed by flow cytometry, DNA adduct formation was assessed by 32P-postlabeling analysis, microarrays of 44K human genome-wide oligos and RT-PCR were used to detect gene expression (mRNA changes and Western blotting was performed to determine the expression of some proteins, including cytochrome P450 (CYP 1A1 and CYP1B1, which are involved in BaP metabolism. Results Following BaP exposure, cells evaded G1 arrest and accumulated in S-phase. Higher levels of DNA damage occurred in S- and G2/M- compared with G0/G1-enriched cultures. Genes that were found to have altered expression included those involved in xenobiotic metabolism, apoptosis, cell cycle regulation and DNA repair. Gene ontology and pathway analysis showed the involvement of various signalling pathways in response to BaP exposure, such as the Catenin/Wnt pathway in G1, the ERK pathway in G1 and S, the Nrf2 pathway in S and G2/M and the Akt pathway in G2/M. An important finding was that higher levels of DNA damage in S- and G2/M-enriched cultures correlated with higher levels of CYP1A1 and CYP1B1 mRNA and proteins. Moreover, exposure of synchronised MCF-7 cells to BaP-7,8-diol-9,10-epoxide (BPDE, the ultimate carcinogenic metabolite of BaP, did not result in significant changes in DNA adduct levels at different phases of the cell cycle. Conclusions This study characterised the complex gene response to BaP in MCF-7 cells and revealed a strong correlation between the varying efficiency of BaP metabolism and DNA damage in different phases of the cell

  16. A common carcinogen benzo[a]pyrene causes neuronal death in mouse via microglial activation.

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    Kallol Dutta

    Full Text Available BACKGROUND: Benzo[a]pyrene (B[a]P belongs to a class of polycyclic aromatic hydrocarbons that serve as micropollutants in the environment. B[a]P has been reported as a probable carcinogen in humans. Exposure to B[a]P can take place by ingestion of contaminated (especially grilled, roasted or smoked food or water, or inhalation of polluted air. There are reports available that also suggests neurotoxicity as a result of B[a]P exposure, but the exact mechanism of action is unknown. METHODOLOGY/PRINCIPAL FINDINGS: Using neuroblastoma cell line and primary cortical neuron culture, we demonstrated that B[a]P has no direct neurotoxic effect. We utilized both in vivo and in vitro systems to demonstrate that B[a]P causes microglial activation. Using microglial cell line and primary microglial culture, we showed for the first time that B[a]P administration results in elevation of reactive oxygen species within the microglia thereby causing depression of antioxidant protein levels; enhanced expression of inducible nitric oxide synthase, that results in increased production of NO from the cells. Synthesis and secretion of proinflammatory cytokines were also elevated within the microglia, possibly via the p38MAP kinase pathway. All these factors contributed to bystander death of neurons, in vitro. When administered to animals, B[a]P was found to cause microglial activation and astrogliosis in the brain with subsequent increase in proinflammatory cytokine levels. CONCLUSIONS/SIGNIFICANCE: Contrary to earlier published reports we found that B[a]P has no direct neurotoxic activity. However, it kills neurons in a bystander mechanism by activating the immune cells of the brain viz the microglia. For the first time, we have provided conclusive evidence regarding the mechanism by which the micropollutant B[a]P may actually cause damage to the central nervous system. In today's perspective, where rising pollution levels globally are a matter of grave concern, our

  17. Benzo(a)pyrene Induced p53 Mediated Male Germ Cell Apoptosis: Synergistic Protective Effects of Curcumin and Resveratrol.

    Science.gov (United States)

    Banerjee, Bhaswati; Chakraborty, Supriya; Ghosh, Debidas; Raha, Sanghamitra; Sen, Parimal C; Jana, Kuladip

    2016-01-01

    Benzo(a)pyrene (B(a)P) is an environmental toxicant that induces male germ cell apoptosis. Curcumin and resveratrol are phytochemicals with cytoprotective and anti-oxidative properties. At the same time resveratrol is also a natural Aryl hydrocarbon Receptor (AhR) antagonist. Our present study in isolated testicular germ cell population from adult male Wistar rats, highlighted the synergistic protective effect of curcumin and resveratrol against B(a)P induced p53 mediated germ cell apoptosis. Curcumin-resveratrol significantly prevented B(a)P induced decrease in sperm cell count and motility, as well as increased serum testosterone level. Curcumin-resveratrol co-treatment actively protected B(a)P induced testicular germ cell apoptosis. Curcumin-resveratrol co-treatment decreased the expression of pro-apoptotic proteins like cleaved caspase 3, 8 and 9, cleaved PARP, Apaf1, FasL, tBid. Curcumin-resveratrol co-treatment decreased Bax/Bcl2 ratio, mitochondria to cytosolic translocation of cytochrome c and activated the survival protein Akt. Curcumin-resveratrol decreased the expression of p53 dependent apoptotic genes like Fas, FasL, Bax, Bcl2, and Apaf1. B(a)P induced testicular reactive oxygen species (ROS) generation and oxidative stress were significantly ameliorated with curcumin and resveratrol. Curcumin-resveratrol co-treatment prevented B(a)P induced nuclear translocation of AhR and CYP1A1 (Cytochrome P4501A1) expression. The combinatorial treatment significantly inhibited B(a)P induced ERK 1/2, p38 MAPK and JNK 1/2 activation. B(a)P treatment increased the expression of p53 and its phosphorylation (p53 ser 15). Curcumin-resveratrol co-treatment significantly decreased p53 level and its phosphorylation (p53 ser 15). The study concludes that curcumin-resveratrol synergistically modulated MAPKs and p53, prevented oxidative stress, regulated the expression of pro and anti-apoptotic proteins as well as the proteins involved in B(a)P metabolism thus protected germ

  18. An investigation of endocrine disrupting effects and toxic mechanisms modulated by benzo[a]pyrene in female scallop Chlamys farreri

    Energy Technology Data Exchange (ETDEWEB)

    Tian, Shuangmei; Pan, Luqing, E-mail: panlq@ouc.edu.cn; Sun, Xiaohua

    2013-11-15

    Highlights: •B[a]P disturbed progesterone, 17β-estradiol and testosterone production in scallop. •B[a]P inhibited 3β-HSD, CYP17 and 17β-HSD expression after a 10-day exposure. •B[a]P of lower dose elevated AHR-CYP1A expression but high dose B[a]P inhibited them. •ER and vitellogenin transcription was consistent with AHR after B[a]P exposure. •B[a]P exposure induced relatively developmental delay and impairment of ovary. -- Abstract: The purpose of this study was to investigate the endocrine disrupting effects induced by benzo[a]pyrene (B[a]P) and explore the underlying mechanisms in mollusks. In this study, sexually mature female Chlamys farreri were exposed to benzo[a]pyrene for 10 days at four different concentrations as 0, 0.025, 0.5 and 10 μg/L. Sex steroids were identified and quantified by electrochemiluminescence immunoassay (ECLIA) method and results showed that exposure to B[a]P exerts great suppression on 17β-estradiol, testosterone production and disrupts progesterone levels in ovary. Transcription of genes were detected and measured by real-time RT-PCR. It showed that at day 10 B[a]P inhibited 3β-HSD, CYP17 and 17β-HSD mRNA expression in a dose-dependent manner, which suggests that they could be potential targets of B[a]P that disrupt steroidogenic machinery. Moreover, 0.025 μg/L B[a]P activated transcription of aryl hydrocarbon receptor (AHR), AHR nuclear translocator (ARNT), CYP1A1 and estrogen receptor (ER), while 10 μg/L B[a]P suppressed all of them. The consistency of their responses to B[a]P exposure implies that AHR action may be involved in invertebrate CYP regulation and ER transcription despite of unknown mechanisms. Additionally, B[a]P exposure could induce ovarian impairment and developmental delay in C. farreri. Overall, sensitivity of C. farreri to endocrine disruption and toxicity suggests that C. farreri is a suitable species for study of endocrine-disrupting effects in marine invertebrates. This study will form a

  19. Does chewing gum improve recovery after an abdominal surgery? –First update

    Directory of Open Access Journals (Sweden)

    Gabriel Rada

    2015-06-01

    Full Text Available Este resumen Epistemonikos (Living FRISBEE: Living FRIendly Summary of the Body of Evidence using Epistemonikos es una actualización del resumen publicado en Noviembre de 2014, basado en 4 nuevas revisiones sistemáticas aparecidas con posterioridad. El íleo postoperatorio es una condición común que retrasa la recuperación luego de una cirugía abdominal. El uso precoz de goma de mascar, como método de alimentación fingida, estimularía la peristalsis permitiendo una alimentación más precoz. Utilizando la base de datos Epistemonikos, la cual es mantenida mediante búsquedas en 30 bases de datos, identificamos 18 revisiones sistemáticas que en conjunto incluyen 81 estudios aleatorizados. Realizamos un metanálisis y tablas de resumen de los resultados utilizando el método GRADE. Concluimos que la goma de mascar probablemente disminuye el tiempo de hospitalización luego de una cirugía abdominal.

  20. USO DE ESCHERICHIA COLI PARA BIORREMEDIACIÓN DE EFLUENTES CONTAMINADOS POR CROMO (VI

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    María C. Panigatti

    2012-01-01

    Full Text Available El objetivo del trabajo fue estudiar el uso de la Escherichia coli en la detoxificación de aguas residuales con Cr (VI perteneciente a una planta metalmecánica. Se evaluó el crecimiento y desarrollo de E. coli con distintas concentraciones de Cr (VI, en diferentes tiempos y condiciones de trabajo. Se estudió la biorreducción de cromo utilizando la cepa en estudio, en diferentes soportes y se evaluó la influencia de la presencia de metales tales como plomo, níquel y zinc. La bacteria fue capaz de reducir Cr(VI trabajando con concentraciones de hasta 200 mg . L-1. Se comprobó la adaptabilidad de la cepa para adoptar como mecanismo de detoxificación, la biorreducción del metal pesado. La presencia de otros metales como plomo, níquel y zinc retrasa el proceso, pero no lo inhibe totalmente. Los resultados obtenidos demuestran la posibilidad de utilizar E. coli en la biorremediación de un efluente industrial conteniendo Cr(VI.

  1. Del tabaco al uso de otras drogas: ¿el uso temprano de tabaco aumenta la probabilidad de usar otras drogas?

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    Medina-Mora Ma. Elena

    2002-01-01

    Full Text Available Objetivo. Analizar la probabilidad de abuso de sustancias en relación con la edad de inicio del consumo de tabaco. Material y métodos. Los datos provienen de la Encuesta Nacional de Adicciones (1998 realizada en población urbana. Resultados. La edad de mayor riesgo para experimentar con sustancias es entre los 15 y los 19 años. Solamente en 5.6% de los usuarios de drogas y 13% de los que han consumido alcohol, el uso del tabaco no ocurrió primero. La probabilidad de beber en forma consuetudinaria y de presentar dependencia es mayor cuando la edad de inicio es temprana y disminuye en la medida en que se retrasa la edad de inicio. La experimentación con drogas, el continuar usándolas y el poliuso son más frecuentes entre quienes se iniciaron antes de los 15 años. Conclusiones. El inicio temprano en el consumo de tabaco incrementa la probabilidad de uso y abuso de sustancias.

  2. Metabolic enzyme activities, metabolism-related genes expression and bioaccumulation in juvenile white shrimp Litopenaeus vannamei exposed to benzo[a]pyrene.

    Science.gov (United States)

    Ren, Xianyun; Pan, Luqing; Wang, Lin

    2014-06-01

    The purpose of this study was to investigate the impact of benzo[a]pyrene (BaP) on metabolic detoxification system and bioaccumulation of white shrimp Litopenaeus vannamei. In this study, juvenile white shrimp L. vannamei were exposed for 21 days at four different concentrations of 0, 0.03, 0.3 and 3μg/L. Detoxification enzyme activities of phase I (aryl hydrocarbon hydroxylase (AHH), 7-ethoxyresorufin O-deethylase (EROD), epoxide hydrolase (EH)) and phase II (glutathione-S-transferase (GST), sulfotransferase (SULT), uridine diphosphate glucuronyl transferase (UGT)) were determined, and results showed that all the detoxification enzyme activities increased in a dose-dependent manner except for the low BaP exposure. Transcription of genes was detected and measured by real-time RT-PCR. It showed that at day six BaP increased cytochrome P450 (CYP) 1A1, GST, SULT visa aryl hydrocarbon receptor (AhR) mRNA expression in a dose-dependent manner, which suggests that they could be potential targets of BaP that disrupt the detoxification system. The consistency of their responses to BaP exposure implies that AhR action may be involved in invertebrate CYP regulation. Additionally, BaP bioaccumulation increased rapidly first and showed an incoming plateau. Besides, the enzyme activities and bioaccumulation in the hepatopancreas were higher than those in the gills. These results will not only provide information on BaP metabolic mechanism for this species, but also scientific data for pollution monitoring. PMID:24636950

  3. In vitro Study on Role of Hsp70 Expression in DNA Damage of Human Embryonic Lung Cells Exposed to Benzo[a]pyrene

    Institute of Scientific and Technical Information of China (English)

    YA-JUAN GAO; CHENG-FENG XIAO; SHENG CHEN; RUI-BO WANG; HAN-ZHEN HE; ROBERT M TANGUAY; TANG-CHUN WU

    2004-01-01

    Objective Benzo[a]pyrene (B[a]P), a ubiquitous environmental pollutant, is a potent procarcinogen and mutagen that can elicit tumors, leading to malignancy. Heat shock proteins (Hsp) have been shown to protect cells against damages caused by various stresses including exposure to numerous chemicals. Whether Hsps, or more specifically Hsp70, are involved in repair of B[a]P-induced DNA damage is currently unknown. Methods We assessed the potential role of the inducible form of Hsp70 in B[a]P-induced DNA damage of human embryonic lung (HEL) cells using immunoblot and the comet assay (i.e., the single cell gel electrophoresis assay). Results Exposure to B[a]P induced a dose-dependent decrease in the level of Hsp70, but a dose-dependent increase in DNA damage both in untreated (control) HEL cells and in cells preconditioned by a heat treatment. Heat preconditioning prior to B[a]P exposure potentiated the effect of B[a]P at a low dose (10 (mol/L), but appeared to be protective at higher doses. There was a negative correlation between Hsp70 level and DNA damage in the non-preheated as well as in the preconditioned cells. Conclusion These data suggest that exposure of HEL cells to B[a]P may induce a dose-dependent reduction in the levels of the inducible Hsp70. The detailed mechanisms for the reduction of Hsp70 levels by B[a]P and the role of Hsp70 in DNA damage under different concentrations of B[a]P remains to be determined.

  4. When father doesn't know best: selective disagreement between self-report and informant report of the broad autism phenotype in parents of a child with autism.

    Science.gov (United States)

    Sasson, Noah J; Faso, Daniel J; Parlier, Morgan; Daniels, Julie L; Piven, Joseph

    2014-12-01

    The Broad Autism Phenotype Questionnaire (BAPQ) is a reliable tool for identifying three autism-related traits-social aloofness, pragmatic language abnormalities and rigid personality--within families of a person with autism and the general population. Although little is known concerning agreement between self-report and informant report versions of the BAPQ, identifying individual characteristics affecting agreement between the two can highlight important considerations for maximizing its yield, particularly when only one version is administered. Here, analysis of self-report and informant report of the BAPQ completed by 444 parents of a child with autism revealed moderate to strong agreement between the two versions for all three broad autism phenotype (BAP) traits when the self-reporting parent did not possess the trait being assessed. In contrast, disagreement selectively occurred when the assessed parent was positive for the BAP trait being rated. This pattern was driven primarily by fathers who were positive for a BAP trait endorsing lower levels of that trait relative to informant report. This discrepancy did not occur for mothers, nor did it occur for fathers lacking BAP traits. Because this pattern was specific to fathers positive for BAP traits, it likely reflects selective "blind spots" in their self-reporting and not poorer self-reporting by fathers more broadly, nor a general tendency of overreporting by informant mothers. The presence of BAP traits in informing parents, however, largely did not reduce agreement between self-report and informant report. In sum, self-report may underestimate the presence of BAP traits in fathers but is generally consistent with informant report for mothers.

  5. Rapid determination of benzo[a]pyrene by membrane enrichment coupled with solid-phase constant-wavelength synchronous fluorescence spectrometry.

    Science.gov (United States)

    Wang, Lei; Li, Wei; Zhang, Feiyu; Li, Hui; Cai, Guimin; Li, Bing; Qian, Guangbei; Du, Yiping

    2016-09-01

    Normal methods for benzo[a]pyrene (BaP) determination, including gas chromatography-mass spectrometry and liquid chromatography with fluorescence detection, involve expensive instruments and complex separation and purification processes. Based on membrane enrichment, coupled with solid-phase constant-wavelength synchronous fluorescence spectrometry, a simple, fast, sensitive method was proposed for the determination of BaP in water samples. A Nylon membrane was used as the solid-phase extraction material for enrichment. After enrichment, a constant-wavelength synchronous fluorescence spectrum was scanned directly on the membrane-concentrated BaP without elution. Spectral measurement and enrichment conditions were optimized. Under optimum conditions, when using 150 mL sample solutions, the relationship between fluorescence intensity and BaP concentrations in the 0.05-10.00 μg L(-1) range could be fitted by binomial function with an R(2) value of 0.9973. Limit of detection (LOD) was calculated to be 0.0137 μg L(-1) . The volume of the sample solution was increased to 1000 mL to test if the method could be applied to determine lower BaP concentrations. A linear relationship still existed in the range 2.0-20.0 ng L(-1) BaP with an R(2) value of 0.9895, and a LOD of 2.4 ng L(-1) . The method was also used to measure the BaP concentration in several natural water samples, and recoveries were in the 90-110% range with relative standard deviations (RSDs) in the 0.58-7.93% range. Copyright © 2016 John Wiley & Sons, Ltd. PMID:26781918

  6. Drug Redeployment to Kill Leukemia and Lymphoma Cells by Disrupting SCD1-Mediated Synthesis of Monounsaturated Fatty Acids.

    Science.gov (United States)

    Southam, Andrew D; Khanim, Farhat L; Hayden, Rachel E; Constantinou, Julia K; Koczula, Katarzyna M; Michell, Robert H; Viant, Mark R; Drayson, Mark T; Bunce, Chris M

    2015-06-15

    The redeployed drug combination of bezafibrate and medroxyprogesterone acetate (designated BaP) has potent in vivo anticancer activity in acute myelogenous leukemia (AML) and endemic Burkitt lymphoma (eBL) patients; however, its mechanism-of-action is unclear. Given that elevated fatty acid biosynthesis is a hallmark of many cancers and that these drugs can affect lipid metabolism, we hypothesized that BaP exerts anticancer effects by disrupting lipogenesis. We applied mass spectrometry-based lipidomics and gene and protein expression measurements of key lipogenic enzymes [acetyl CoA carboxylase 1 (ACC1), fatty acid synthase (FASN), and stearoyl CoA desaturase 1 (SCD1)] to AML and eBL cell lines treated with BaP. BaP treatment decreased fatty acid and phospholipid biosynthesis from (13)C D-glucose. The proportion of phospholipid species with saturated and monounsaturated acyl chains was also decreased after treatment, whereas those with polyunsaturated chains increased. BaP decreased SCD1 protein levels in each cell line (0.46- to 0.62-fold; P < 0.023) and decreased FASN protein levels across all cell lines (0.87-fold decrease; P = 1.7 × 10(-4)). Changes to ACC1 protein levels were mostly insignificant. Supplementation with the SCD1 enzymatic product, oleate, rescued AML and e-BL cells from BaP cell killing and decreased levels of BaP-induced reactive oxygen species, whereas supplementation with the SCD1 substrate (and FASN product), palmitate, did not rescue cells. In conclusion, these data suggest that the critical anticancer actions of BaP are decreases in SCD1 levels and monounsaturated fatty acid synthesis. To our knowledge, this is the first time that clinically available antileukemic and antilymphoma drugs targeting SCD1 have been reported. PMID:25943877

  7. Application of new balloon catheters in the treatment of congenital heart defects

    Science.gov (United States)

    Fiszer, Roland; Szkutnik, Małgorzata; Smerdziński, Sebastian; Chodór, Beata; Białkowski, Jacek

    2016-01-01

    Introduction Balloon angioplasty (BAP) and aortic or pulmonary balloon valvuloplasty (BAV, BPV) are well-established treatment options in congenital heart defects. Recently, significant technological progress has been made and new catheters have been implemented in clinical practice. Aim To analyze the results of BAP, BAV and BPV with the new balloon catheter Valver and its second generation Valver II, which the company Balton (Poland) launched and developed. These catheters have not been clinically evaluated yet. Material and methods We performed 64 interventions with Valver I and Valver II. With Valver I the following procedures were performed: 17 BPV (including 9 in tetralogy of Fallot – TOF), 10 BAV and 27 BAP in coarctations of the aorta (CoA) – including 9 native and 18 after surgery. With Valver II ten interventions were done – 3 BPV, 2 pulmonary supravalvular BAP (after switch operations), 2 BAP of recoarctations and 3 other BAP. Age of the patients ranged from a few days to 40 years. Results All procedures were completed successfully, without rupture of any balloon catheters. The pressure gradient drop was statistically significant in all groups: BPV in isolated pulmonary valvular stenosis 28.1 mm Hg (mean), BPV in TOF 18.7 mm Hg, BAV 32.8 mm Hg, BAP in native CoA 15.4 mm Hg and in recoarctations 18.6 mm Hg. In 3 cases during rapid deflation of Valver I, wrinkles of the balloons made it impossible to insert the whole balloon into the vascular sheath (all were removed surgically from the groin). No such complication occured with Valver II. Conclusions Valver balloon catheters are an effective treatment modality in different valvular and vascular stenoses. PMID:27625686

  8. 食用植物油中苯并(a)芘来源及形成机理的研究进展%Advance in source and formation mechanism of benzo(a) pyrene in edible vegetable oil

    Institute of Scientific and Technical Information of China (English)

    刘国艳; 张振芳; 金青哲; 王兴国; 徐鑫

    2013-01-01

    近年来食用植物油中强致癌物苯并(a)芘(BaP)超标事件的相继报道,引起政府和公众的强烈关注.根据已有文献报道,总结食用植物油加工过程中BaP的主要来源及迁移变化情况,并结合非食品领域BaP形成机理的报道,分析食用植物油中BaP的形成机理,为食用植物油中BaP来源和形成机理的进一步研究提供参考,以利于有效控制食用植物油中BaP含量方法的建立,达到降低和控制食用植物油中BaP的污染,保证人体健康的目的.%In recent years, the excessive content of strong carcinogenicity benzo(a) pyrene (BaP) in edible vegetable oil has been reported, which causes strong concern of the government and the public. On the basis of other literatures, the main sources, migration and variation of BaP in edible vegetable oil during oil processing were summarized. Combining with the reported BaP formation mechanism in non - food field, the BaP formation mechanism in edible vegetable oil was analyzed, which provided references for further research of BaP in edible vegetable oil on the source and formation mechanism, so it was conducive to establish effective method for controlling BaP content in edible vegetable oil, and then the purpose of reducing and controlling BaP pollution in edible vegetable oil could be achieved.

  9. Effects of N(6)-benzylaminopurine and Indole Acetic Acid on In Vitro Shoot Multiplication, Nodule-like Meristem Proliferation and Plant Regeneration of Malaysian Bananas (Musa spp.).

    Science.gov (United States)

    Sipen, Philip; Davey, Michael R

    2012-12-01

    Different concentrations of N(6)-benzylaminopurine (BAP) and indole acetic acid (IAA) in Murashige and Skoog based medium were assessed for their effects on shoot multiplication, nodule-like meristem proliferation and plant regeneration of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak. BAP at 1-14 mg L(-1) with or without 0.2 mg L(-1) IAA, or BAP at 7-14 mg L(-1) with the same concentration of IAA, was evaluated for shoot multiplication from shoot tips and the proliferation of nodule-like meristems from scalps, respectively. Plant regeneration from scalps was assessed using 1 mg L(-1) BAP and 0.2 mg L(-1) IAA separately, or a combination of these two growth regulators. Data on shoot multiplication, the proliferation of nodule-like meristems with associated plant regeneration were recorded after 30 days of culture. A maximum of 5 shoots per original shoot tip was achieved on medium supplemented with BAP at 5 mg L(-1) (Pisang Nangka), 6 mg L(-1) (Pisang Mas and Pisang Berangan), or 7 mg L(-1) (Pisang Awak), with 0.2 mg L(-1) IAA. BAP at 11 mg L(-1) with 0.2 mg L(-1) IAA induced the most highly proliferating nodule-like meristems in the four banana cultivars. Plant regeneration from scalps was optimum in all cases on medium containing 1 mg L(-1) BAP and 0.2 mg L(-1) IAA. This is the first report on the successful induction of highly proliferating nodule-like meristems and plant regeneration from scalps of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak.

  10. Electrodermal screening of biologically active points for upper gastrointestinal bleeding.

    Science.gov (United States)

    Tseng, Ying-Jung; Hu, Wen-Long; Hung, I-Ling; Hsieh, Chia-Jung; Hung, Yu-Chiang

    2014-01-01

    The purpose of this case-control study was to investigate the relationship between the electrical resistance of the skin at biologically active points (BAPs) on the main meridians and upper gastrointestinal bleeding (UGIB). Electrical resistance to direct current at 20 BAPs on the fingers and toes of 100 patients with (38 men, 12 women; mean age [range], 58.20 ± 19.62 [18-83] years) and without (27 men, 23 women; 49.54 ± 12.12 [22-74] years) UGIB was measured through electrodermal screening (EDS), based on the theory of electroacupuncture according to Voll (EAV). Data were compared through analysis of variance (ANOVA), receiver operating characteristic (ROC) curve analysis, and logistic regression. The initial readings were lower in the UGIB group, indicating blood and energy deficiency due to UGIB. Significant differences in indicator drop values were observed at nine BAPs (p < 0.05) on the bilateral small intestine, bilateral stomach, bilateral circulation, bilateral fibroid degeneration, and right lymph meridians. The area under the ROC curve values of the BAPs on the bilateral small intestine and stomach meridians were larger than 0.5, suggesting the diagnostic accuracy of EDS for UGIB on the basis of the indicator drop of these BAPs. Logistic regression revealed that when the indicator drop of the BAP on the left stomach meridian increased by one score, the risk of UGIB increased by about 1.545-3.523 times. In conclusion, the change in the electrical resistance of the skin measured by EDS at the BAPs on the bilateral small intestine and stomach meridians provides specific information on UGIB.

  11. Absence of formation of benzo[a]pyrene/DNA adducts in the cuttlefish (Sepia officinalis, Mollusca: Cephalopoda)

    Energy Technology Data Exchange (ETDEWEB)

    Lee, P.G.; Lu, L.J.W.; Salazar, J.J.; Holoubek, V. (Univ. of Texas Medical Branch, Galveston, TX (United States))

    1994-01-01

    Benzo[a]pyrene (B[a]P) injected intramuscularly into the base of the arms of cuttlefish was released continuously from the injection site and removed from the organism. Only a portion of the compound accumulated in the body. Twenty-four hr after its injection, 75% of B[a]P applied in olive oil was removed from the cuttlefish, and 1.2% was found in the body outside the head, in site of injection. If the carcinogen was dissolved in dimethylformamide, the removal of B[a]P was slower, so that only 18% of the injected B[a]P was removed from the organism and 0.36% accumulated in the body outside the head 24 hr after injection. The high level of B[a]P in gills and hemolymph 4 hr after injection and the kinetics of the decrease of its concentration with time indicate that these two organs could be involved in the excretion of B[a]P from the body. The B[a]P/DNA adducts characteristic for vertebrates could not be demonstrated in gills, skin, brain, hepatopancreas, and lymphocytes of the cuttlefish 24 hr after injection. The dose of the carcinogene injected into the cuttlefish was 2-4 times higher than the dose resulting in the formation of a high level of B[a]P/DNA adducts in vertebrates. A different metabolism of B[a]P in the tissue of cephalopods, compared to vertebrates, could be less favorable to the process leading to malignant transformation and could explain the absence from the literature of reports of tumors in cephalopods. 15 refs., 1 fig., 2 tabs.

  12. Benzo[a]pyrene affects Jurkat T cells in the activated state via the antioxidant response element dependent Nrf2 pathway leading to decreased IL-2 secretion and redirecting glutamine metabolism

    Energy Technology Data Exchange (ETDEWEB)

    Murugaiyan, Jayaseelan; Rockstroh, Maxie; Wagner, Juliane [Department of Proteomics, Helmholtz-Centre for Environmental Research — UFZ, Permoserstr. 15, 04318 Leipzig (Germany); Baumann, Sven [Department of Metabolomics, Helmholtz-Centre for Environmental Research — UFZ, Permoserstr. 15, 04318 Leipzig (Germany); Schorsch, Katrin [Department of Proteomics, Helmholtz-Centre for Environmental Research — UFZ, Permoserstr. 15, 04318 Leipzig (Germany); Trump, Saskia; Lehmann, Irina [Department of Environmental Immunology, Helmholtz-Centre for Environmental Research — UFZ, Permoserstr. 15, 04318 Leipzig (Germany); Bergen, Martin von [Department of Proteomics, Helmholtz-Centre for Environmental Research — UFZ, Permoserstr. 15, 04318 Leipzig (Germany); Department of Environmental Immunology, Helmholtz-Centre for Environmental Research — UFZ, Permoserstr. 15, 04318 Leipzig (Germany); Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg (Denmark); Tomm, Janina M., E-mail: Janina.tomm@ufz.de [Department of Proteomics, Helmholtz-Centre for Environmental Research — UFZ, Permoserstr. 15, 04318 Leipzig (Germany)

    2013-06-15

    There is a clear evidence that environmental pollutants, such as benzo[a]pyrene (B[a]P), can have detrimental effects on the immune system, whereas the underlying mechanisms still remain elusive. Jurkat T cells share many properties with native T lymphocytes and therefore are an appropriate model to analyze the effects of environmental pollutants on T cells and their activation. Since environmental compounds frequently occur at low, not acute toxic concentrations, we analyzed the effects of two subtoxic concentrations, 50 nM and 5 μM, on non- and activated cells. B[a]P interferes directly with the stimulation process as proven by an altered IL-2 secretion. Furthermore, B[a]P exposure results in significant proteomic changes as shown by DIGE analysis. Pathway analysis revealed an involvement of the AhR independent Nrf2 pathway in the altered processes observed in unstimulated and stimulated cells. A participation of the Nrf2 pathway in the change of IL-2 secretion was confirmed by exposing cells to the Nrf2 activator tBHQ. tBHQ and 5 μM B[a]P caused similar alterations of IL-2 secretion and glutamine/glutamate metabolism. Moreover, the proteome changes in unstimulated cells point towards a modified regulation of the cytoskeleton and cellular stress response, which was proven by western blotting. Additionally, there is a strong evidence for alterations in metabolic pathways caused by B[a]P exposure in stimulated cells. Especially the glutamine/glutamate metabolism was indicated by proteome pathway analysis and validated by metabolite measurements. The detrimental effects were slightly enhanced in stimulated cells, suggesting that stimulated cells are more vulnerable to the environmental pollutant model compound B[a]P. - Highlights: • B[a]P affects the proteome of Jurkat T cells also at low concentrations. • Exposure to B[a]P (50 nM, 5 μM) did not change Jurkat T cell viability. • Both B[a]P concentrations altered the IL-2 secretion of stimulated cells.

  13. Modulatory effects of Azadirachta indica on benzo(a)pyrene-induced forestomach tumorigenesis in mice

    Institute of Scientific and Technical Information of China (English)

    Subhash Chander Gangar; Rajat Sandhir; Durg Vijay Rai; Ashwani Koul

    2006-01-01

    AIM: To evaluate the chemopreventive effects of aqueous Azadirachta indica (A indica) leaf extract (AAILE)against benzo(a)pyrene [B(a)P]-induced forestomach tumorigenesis in Balb/c mice.METHODS: Female Balb/c mice were divided into four groups of 10-12 animals each. For induction of forestomach tumors, starting from d 14 of the experiment, mice of B(a)P and B(a)P+A indica groups were given intra-gastric instillations of B(a)P (40 mg/kg), twice a week for four weeks. Mice of A indica and B(a)P+A indica groups were orally administered with AAILE (100mg/kg), two weeks prior to B(a)P instillations till the end of the experiment. After 22 wk of the first B(a)P instillation, mice were sacrificed and the forestomachs were analyzed for development of tumors, scanning electron microscopy (SEM) and histopathology.RESULTS: Tumor incidence was observed to be 100%in mice that received only B(a)P. However, treatment with AAILE reduced the tumor incidence by 58.4%as observed in mice of B(a)P+A indica group when compared to that of B(a)P group. Similarly, the tumor burden and multiplicity were seen to decrease by 87.3% and 69.6% respectively in mice of B(a)P+A indica group when compared to those of B(a)P group.Scanning electron microscopy analysis showed that AAILE treatment itself did not cause any abnormalities on the surface architecture of forestomach epithelium.In tumorous forestomach, surface disruption was observed. Over the forestomach tumors of B(a)P group of mice certain rounded structures were seen in addition to closely placed tongue-shaped squamous cells. Interestingly, these rounded structures were not observed in B(a)P + A indica group of mice.Histopathalogically, the tumors were identical and diagnosed to be papillomas. Mice from control and A indica groups of mice did not develop any forestomach tumors and showed normal histo-architecture.CONCLUSION: The present data suggest that A indica exerts chemopreventive effects against B(a)P-induced forestomach tumors in

  14. Virus Infection

    Directory of Open Access Journals (Sweden)

    Hiroshi Abe

    2013-01-01

    Full Text Available Of 168 patients with chronic hepatitis B virus (HBV infection-related liver disease, 20 patients who had received 100 mg of lamivudine plus 10 mg/day of adefovir dipivoxil (ADV (ADV group and 124 patients who had received 0.5 mg/day of entecavir or 100 mg/day of lamivudine (non-ADV group for >1 year were enrolled. For comparative analyses, 19 well-matched pairs were obtained from the groups by propensity scores. At the time of enrollment, serum creatinine and phosphate concentrations were similar between the ADV and non-ADV groups; however, urinary phosphate ( and serum bone-specific alkaline phosphatase (BAP ( concentrations were significantly higher in the ADV group than in the non-ADV group. Serum BAP was significantly higher at the time of enrollment than before ADV administration in the ADV group (, although there was no significant change in serum BAP concentration in the non-ADV group. There was a significant positive correlation between the period of ADV therapy and ΔBAP (, . Serum BAP concentration increased before increase in serum creatinine concentration and was useful for early detection of adverse events and for developing adequate measures for continuing ADV for chronic HBV infection-related liver disease.

  15. The effect of plant growth regulators on callus induction somatic embryogenesis of hybird tomato

    International Nuclear Information System (INIS)

    Efficient tissue culture system is important for transformation of important genes in hybrid tomato cultivars. The present study was undertaken to develop an efficient tissue culture system for hybrid tomato cultivar Peto-86. The young primary leaves and stems were inoculated into five different MS media having different concentrations of plant growth regulators in different combinations for callus induction, somatic embryogenesis and for both direct and indirect regeneration. Maximum callus induction frequency 90 percentage was achieved with MS media containing 2,4-D 4 mg L-1 and BAP 0.5 mg L-1. The direct somatic embryogenesis was found highest on MS media supplemented with 2,4-D 4 mg L-1 and BAP 0.5 mg L-1. Maximum indirect regeneration frequency 87 percentage was achieved from primary leaves explants with MS media containing IAA 0.5 mg L-1 and BAP 3 mg L-1 and highest direct regeneration frequency 77% was obtained from primary leaves explants with MS media containing NAA 1 mg L-1 and BAP 3 mg L-1. The high concentration of 2,4-D increased callus induction and somatic embryogenesis frequencies while the high concentration of BAP increased regeneration frequency. An improved tissue culture system of hybrid tomato cultivar Peto-86 was established and it may be recommended for further transformation experiments. (author)

  16. Endocrine disruptive actions of inhaled benzo(a)pyrene on ovarian function and fetal survival in fisher F-344 adult rats.

    Science.gov (United States)

    Archibong, Anthony E; Ramesh, Aramandla; Inyang, Frank; Niaz, Mohammad S; Hood, Darryl B; Kopsombut, Prapaporn

    2012-12-01

    This study evaluated the effect of inhaled BaP on female reproductive function. Rats were exposed to 50, or 75 or 100 μg BaP/m(3), 4 h a day for 14 days via inhalation. Plasma E(2), P(4), LH and FSH concentrations were determined. Ovarian BaP metabolism and aryl hydrocarbon hydrolase (AHH) activity at proestrus were determined and fertility evaluations were conducted. Ovulation rate and number of pups/litter were reduced in rats exposed to 100 μg BaP/m(3) compared with other treatment and control groups. Plasma concentrations of E(2), and LH were significantly reduced at proestrus in BaP-exposed versus those of controls whereas those of P(4) were significantly reduced at diestrus I. The activity of AHH in ovarian and liver tissues and concentrations of BaP 7,8-diol and BaP 3,6-dione metabolites increased in an exposure concentration-dependent manner. These data suggest that exposure of rats to BaP prior to mating contributes to reduced ovarian function and fetal survival. PMID:23059060

  17. Characterization of soluble microbial products in a drinking water biological aerated filter.

    Science.gov (United States)

    Kang, Jia; Ma, Teng-Fei; Zhang, Peng; Gao, Xu; Chen, You-Peng

    2016-05-01

    Utilization-associated products (UAPs) and biomass-associated products (BAPs) were quantified separately in this study to characterize soluble microbial products (SMPs) in a drinking water lab-scale biological aerated filter (BAF), and their basic characteristics were explored using gel filtration chromatography and three-dimensional excitation-emission matrix (3D-EEM) spectrophotometry with fluorescence regional integration analysis and parallel factor model. UAPs were observed increased with the increase of filter media depth and accumulated after BAF treatment, whereas BAPs were basically constant. 3D-EEM spectroscopy analysis result showed that tryptophan and protein-like compounds were the main components of UAPs and BAPs, and fulvic-acid-like substance was a major component of BAPs, rather than UAPs. In terms of molecular weight (MW) distribution, UAP MW presented a bimodal distribution in the range of 1-5 and >10 kDa, while BAP MW exhibited unimodal distribution with MW >20 kDa fraction accounting for more than 90 %. The macromolecules of UAPs accumulated after BAF treatment. This study provides theoretical support for in-depth study of SMP characteristics. PMID:26801929

  18. Effects of benzo(a)pyrene exposure on the antioxidant enzyme activity of scallop Chlamys farreri

    Science.gov (United States)

    Pan, Luqing; Ren, Jiayun; Zheng, Debin

    2009-02-01

    Scallop Chlamys farreri was exposed to different concentrations of benzo(a)pyrene (BaP) (0.5 μg/L, 1.0 μg/L, 10.0 μg/L and 50.0 μg/L) for 30 days in seawater. The 7-ethoxyresorufin O-deethylase (EROD) activity was significantly induced, and increased with the increasing BaP concentration. The glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), Glutathione peroxidase (GPx) activities increased in short time at low concentration of BaP, and was significantly depressed at high concentrations. Scallop gill was more sensitive to BaP than the digestive gland, and the digestive gland was the main tissue to deal with oxyradicals. The contents of malondialdehyde (MDA) increased with the exposure time and there was a positive correlation (concentration-effect) between the MDA content and the concentration of BaP. The biomarkers determined in this experiment had important roles in detoxification, and showed great potential as biomarkers for oxidative stress. Controlled laboratory experiments designed to simulate field exposure scenarios are particularly useful in ascertaining biomarkers suitable for use with complex contaminant mixtures in the marine environment.

  19. The application of the comet assay to assess the genotoxicity of environmental pollutants in the nematode Caenorhabditis elegans.

    Science.gov (United States)

    Imanikia, Soudabeh; Galea, Francesca; Nagy, Eszter; Phillips, David H; Stürzenbaum, Stephen R; Arlt, Volker M

    2016-07-01

    This study aimed to establish a protocol for cell dissociation from the nematode Caenorhabditis elegans (C. elegans) to assess the genotoxicity of the environmental pollutant benzo[a]pyrene (BaP) using the alkaline version of the single cell electrophoresis assay (comet assay). BaP genotoxicity was assessed in C. elegans (wild-type [WT]; N2, Bristol) after 48h exposure (0-40μM). Induction of comets by BaP was concentration-dependent up to 20μM; comet% tail DNA was ∼30% at 20μM BaP and ∼10% in controls. Similarly, BaP-induced DNA damage was evaluated in C. elegans mutant strains deficient in DNA repair. In xpa-1 and apn-1 mutants BaP-induced comet formation was diminished to WT background levels suggesting that the damage formed by BaP that is detected in the comet assay is not recognised in cells deficient in nucleotide and base excision repair, respectively. In summary, our study provides a protocol to evaluate DNA damage of environmental pollutants in whole nematodes using the comet assay. PMID:27389785

  20. In vitro study on regeneration of Gladiolus grandiflorus corm calli as affected by plant growth regulators.

    Science.gov (United States)

    Torabi-Giglou, Mousa; Hajieghrari, Behzad

    2008-04-15

    In this study, in vitro organogenesis of Gladiolus grandiflorus cultivar pink corm segments were evaluated by culturing corm calli in modified MS medium supplemented with 3% sucrose and 0.7% agar with different concentration of BAP (0, 1, 2 and 4 mg L(-1) medium) and NAA (0, 0.5, 1 and 2 mg L(-1) medium) in factorial experiment of Completely Randomized Design (CRD). In order to obtain Gladiolus calli, corm segments (Aprox. 5 x 5 x 1 mm in size) were kept in modified MS medium (Murashige and Skoog, 1962) that was supplemented with 1 mg L(-1) 2, 4-D, 3% sucrose and 0.7% agar. The results showed that increasing the concentration of BAP from 0 to 2 mg L(-1) medium simulated plantlet regeneration but no significantly effect was obtained on shoot and cormel organogenesis between 2 and 4 mg L(-1) BAP concentration in medium. Increasing of NAA content in media without BAP developed rootlet significantly. Interaction results showed that increasing BAP content against decreasing of NAA concentration stimulates the shoot and cormel proliferation.

  1. Rapid and sensitive determination of benzo[a]pyrene in black ginseng using fluorescence detector and high performance liquid chromatography-tandem mass spectrometry

    Science.gov (United States)

    Cho, Hyun-jeong; Kim, Hye-jin; Son, Byeong-cheol; Jo, Dong-keun; Cho, Byung-lim

    2013-05-01

    Black ginseng is produced by steaming a ginseng root followed by drying repeatedly 9 times during the process and it is changed to be black color, so it is known that a black ginseng has more contents of saponins than red ginseng. However a fake black ginseng which is produced to be black color at high temperature in a short period of time generate carcinogenic benzo[a]pyrene(BaP) through the process. In this year, maximum residue level(MRL) for BaP was established to 2 ug/kg in black ginseng and more sensitive method was developed to quantitatively analyze the BaP by high performance liquid chromatography (HPLC) coupling with florescence detector and tandem mass spectrometry (atmospheric pressure chemical ionization-MS/MS). Chromatographic separation was performed on a Supelcosil™ LC-PAH column (3 μm, 3 mm x 50 mm). Mobile phase A was water and mobile phase B was acetonitrile. BaP was exactly separated from other 15 polycyclic aromatic hydrocarbons (PAHs) which have been selected as priority pollutants by the US Environmental Protection Agency (EPA). Linearity of detection was in the range of 0.2~20 μg/kg and limit of detection (LOD) for BaP was lower than 0.1 μg/kg, limit of quantification (LOQ) was 0.2 μg/kg. The recovery of Bap was 92.54%+/-6.3% in black ginseng.

  2. 苯并(a)芘对大弹涂鱼肝脏过氧化氢酶活性的影响*%Effects of Catalase Activities in the Liver of Boleophthalmus Pectinirostris with Benzo(a) Pyrene Exposure

    Institute of Scientific and Technical Information of China (English)

    冯涛; 郑微云; 郭祥群; 陈荣

    2001-01-01

    Changes of catalase(CAT) activities in the liver of Boleophthalmus pectinirostris with benzo(a)pyrene(BaP) exposure are detected in experimental condition. The results show that there is no significant changes for CAT activities in control group and 0.05mg*L-1 BaP group with prolonged exposure (P>0.05), whereas they are depressed significantly at concentration of 0.5mg*L-1(P≤0.05). In 3 days exposure, CAT activities decrease significantly with the increase of BaP concentration (P≤0.05). This might indicate that exposure to higher BaP concentrations might have toxic effect to fishes. The activities of CAT increase significantly to control level after BaP was removed. It turned out that the physiological modulatary mechanism still existed in the liver of Boleophthalmus pectinirostris. All the results show that CAT activity might be suitable to be the bioindicator of BaP exposure.

  3. 143例急性胆源性胰腺炎治疗的临床分析%Clinical Analysis on Therapy of 143 Biliary Acute Pancretitis Cases

    Institute of Scientific and Technical Information of China (English)

    于嘉伟; 马利林

    2009-01-01

    目的:探讨急性胆源性胰腺炎(BAP)的临床诊断和手术时机. 方法:2005年6月-2008年12月之间收治的143例BAP患者,根据其是否有胆道梗阻及胰腺是否水肿或坏死分为非梗阻水肿型、非梗阻坏死型、梗阻水肿型以及梗阻坏死型4型,对其治疗方法和临床疗效进行总结分析.结果:非梗阻水肿型BAP 93例和梗阻水肿型BAP 28例均治愈.非梗阻坏死型BAP 11例,治愈10例,死亡1例;梗阻坏死型BAP 11例,治愈7例,死亡4例.结论:手术时机对胆源性急性胰腺炎患者的预后有重大影响,早期宜行积极的保守治疗,根据其具体分型制定个体化治疗方案是减少并发症、提高治愈率的关键.

  4. Seasonal change of non-point source pollution-induced bioavailable phosphorus loss: A case study of Southwestern China

    Science.gov (United States)

    Gao, Yang; Zhu, Bo; Wang, Tao; Wang, Yafeng

    2012-02-01

    SummaryBioavailable phosphorus (P) losses due to agriculture activity in a purple soil watershed in the Sichuan Basin of Southwestern China were monitored to define the hydrological controls of P transport. Our results indicate that the proportion of P that was transported in particulate form increased in the rainy season, and that the mass of total bioavailable P (BAP) loads exhibited seasonal fluctuations, wherein the majority (over 90%) was observed to have been exported between June and September. The proportion of bioavailable dissolved P (BDP) in the BAP discharge budget in the watershed varied between 11% and 15% during the monitoring period. The bioavailable particulate P (BPP) and BDP concentrations of stream water under rainstorm events increased by over 40% in comparison to their annual mean concentrations, and the annual BAP load was primarily dominated by the loads that occurred during rainstorm events in the study year. BAP concentration in groundwater significantly fluctuated with the seasons, and the ratio of total BAP in groundwater to that in surface water gradually increased during the rainy season. Thus, the impact of agriculture on the water quality of this watershed becomes clearly evident.

  5. Electron-accepting potential of solvents determines photolysis rates of polycyclic aromatic hydrocarbons: Experimental and density functional theory study

    Energy Technology Data Exchange (ETDEWEB)

    Shao Jianping [Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024 (China); Chen Jingwen, E-mail: jwchen@dlut.edu.cn [Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024 (China); Xie Qing; Wang Ying; Li Xuehua [Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024 (China); Hao Ce [Carbon Research Laboratory, Center for Nano Materials and Science, School of Chemical Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, Zhongshan Road 158, Dalian 116012 (China)

    2010-07-15

    Photochemical behaviour of polycyclic aromatic hydrocarbons (PAHs) is strongly dependent on the physical and chemical nature of the media in/on which they exist. To understand the media effects, the photolysis of phenanthrene (PHE) and benzo[a]pyrene (BaP) in several solvents was investigated. Distinct photolysis rate constants for PHE and BaP in the different solvents were observed. Some theoretical parameters reflecting the solvent properties were computed and employed to explain the solvent effects. Acetone competitively absorbed light with PHE and BaP, and the excited acetone molecules played different roles for the photodegradation of PHE and BaP. The photolysis rate constants of PHE and BaP in hexane, isopropanol, ethanol, methanol, acetonitrile and dichloromethane were observed to correlate with the electron-accepting potential of the solvent molecules. Absolute electronegativity of the solvents linearly correlated with the photolytic activity (log k) of the PAHs significantly. The results are important for better understanding the photodegradation mechanism of PAHs in different media.

  6. Electron-accepting potential of solvents determines photolysis rates of polycyclic aromatic hydrocarbons: experimental and density functional theory study.

    Science.gov (United States)

    Shao, Jianping; Chen, Jingwen; Xie, Qing; Wang, Ying; Li, Xuehua; Hao, Ce

    2010-07-15

    Photochemical behaviour of polycyclic aromatic hydrocarbons (PAHs) is strongly dependent on the physical and chemical nature of the media in/on which they exist. To understand the media effects, the photolysis of phenanthrene (PHE) and benzo[a]pyrene (BaP) in several solvents was investigated. Distinct photolysis rate constants for PHE and BaP in the different solvents were observed. Some theoretical parameters reflecting the solvent properties were computed and employed to explain the solvent effects. Acetone competitively absorbed light with PHE and BaP, and the excited acetone molecules played different roles for the photodegradation of PHE and BaP. The photolysis rate constants of PHE and BaP in hexane, isopropanol, ethanol, methanol, acetonitrile and dichloromethane were observed to correlate with the electron-accepting potential of the solvent molecules. Absolute electronegativity of the solvents linearly correlated with the photolytic activity (log k) of the PAHs significantly. The results are important for better understanding the photodegradation mechanism of PAHs in different media. PMID:20303660

  7. Effects of benzo(a)pyrene exposure on the antioxidant enzyme activity of scallop Chlamys farreri

    Institute of Scientific and Technical Information of China (English)

    PAN Luqing; REN Jiayun; ZHENG Debin

    2009-01-01

    Scallop Chlamys farreri was exposed to different concentrations of benzo(a)pyrene (BaP) (0.5 μg/L, 1.0 μg/L, 10.0 μg/L and 50.0 μg/L) for 30 days in seawater. The 7-ethoxyresorufin O-deethylase (EROD) activity was significantly induced, and increased with the increasing BaP concentration. The glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), Glutathione peroxidase (GPx) activities increased in short time at low concentration of BaP, and was significantly depressed at high concentrations. Scallop gill was more sensitive to BaP than the digestive gland, and the digestive gland was the main tissue to deal with oxyradicals. The contents of malondialdehyde (MDA) increased with the exposure time and there was a positive correlation (concentration-effect) between the MDA content and the concentration of BaP. The biomarkers determined in this experiment had important roles in detoxification, and showed great potential as biomarkers for oxidative stress. Controlled laboratory experiments designed to simulate field exposure scenarios are particularly useful in ascertaining biomarkers suitable for use with complex contaminant mixtures in the marine environment.

  8. Metabolic biotransformation of copper-benzo[a]pyrene combined pollutant on the cellular interface of Stenotrophomonas maltophilia.

    Science.gov (United States)

    Chen, Shuona; Yin, Hua; Tang, Shaoyu; Peng, Hui; Liu, Zehua; Dang, Zhi

    2016-03-01

    Previous studies have confirmed that Stenotrophomonas maltophilia can bind an appreciable amount of Cu(II) and degrade BaP. However, the removal mechanisms of Cu(II) coexisted with BaP by S. maltophilia are still unclear. In this study, the micro-interaction of contaminants on the cellular surface was investigated. The results indicated that carboxyl groups played an important role in the binding of copper to the thallus and that the cell walls were the main adsorption sites. Nevertheless, these reactive groups had no obvious effect on the uptake of BaP. Instead, the disruption and modification of cell walls accelerated transportation of BaP across the membrane into cells. The observation of SEM-EDS confirmed that Cu(II) would be adsorbed and precipitated onto the cell surface but would also be removed by extracellular precipitation when BaP coexisted. And the XPS analysis reflected that part of Cu(II) bound onto biosorbents changed into Cu(I) and Cu.

  9. Regeneration of Blackgram (Vigna mungo L. on Changes of Hormonal Condition

    Directory of Open Access Journals (Sweden)

    Shariful Alam MONY

    2010-12-01

    Full Text Available The study comprised of experiments for shoot regeneration and plantlet formation from cotyledonary node of Vigna mungo by culturing them on low concentration of BAP followed by transfer to hormone free MS medium. Cotyledonary node explants were cultured on different concentrations of BAP (0, 1, 2.5 and 50 mg l-1. Shoot regeneration occurred from cotyledonary nodes irrespective of the presence or absence of BAP in the medium. However, culture of cotyledonary node explants for 10 days on medium containing 1.0 mg l-1 BAP followed by transfer to hormone free medium gave higher number of shoots (9.33/explant compared to culture of the explants on hormone free medium for 15 days followed by transfer to medium containing 1.0 mg l-1 BAP (8.33/explants. The regenerated shoots were transferred to rooting medium supplemented with different concentrations of IBA and NAA. The high frequency (100.0% of rooting was observed with MS medium supplemented with 0.5 mg l-1 IBA. The rooted plants were transferred to pots for hardening.

  10. Alterations to proteome and tissue recovery responses in fish liver caused by a short-term combination treatment with cadmium and benzo[a]pyrene

    Energy Technology Data Exchange (ETDEWEB)

    Costa, P.M., E-mail: pmcosta@fct.unl.p [IMAR-Instituto do Mar, Departamento de Ciencias e Engenharia do Ambiente, Faculdade de Ciencias e Tecnologia da Universidade Nova de Lisboa, 2829-516 Monte de Caparica (Portugal); Chicano-Galvez, E.; Lopez Barea, J. [Departamento de Bioquimica y Biologia Molecular, Universidad de Cordoba, Campus de Rabanales, Edificio Severo Ochoa, 14071 Cordoba (Spain); DelValls, T.A. [UNESCO/UNITWIN/WiCop Chair-Departamento de Quimica Fisica, Facultad de Ciencias del Mar y Ambientales, Universidad de Cadiz, Poligono rio San Pedro s/n, 11510 Puerto Real, Cadiz (Spain); Costa, M.H. [IMAR-Instituto do Mar, Departamento de Ciencias e Engenharia do Ambiente, Faculdade de Ciencias e Tecnologia da Universidade Nova de Lisboa, 2829-516 Monte de Caparica (Portugal)

    2010-10-15

    The livers of soles (Solea senegalensis) injected with subacute doses of cadmium (Cd), benzo[a]pyrene (B[a]P), or their combination, were screened for alterations to cytosolic protein expression patterns, complemented by cytological and histological analyses. Cadmium and B[a]P, but not combined, induced hepatocyte apoptosis and Kupfer cell hyperplasia. Proteomics, however, suggested that apoptosis was triggered through distinct pathways. Cadmium and B[a]P caused upregulation of different anti-oxidative enzymes (peroxiredoxin and glutathione peroxidase, respectively) although co-exposure impaired induction. Similarly, apoptosis was inhibited by co-exposure, to which may have contributed a synergistic upregulation of tissue metalloproteinase inhibitor, {beta}-actin and a lipid transport protein. The regulation factors of nine out of eleven identified proteins of different types revealed antagonistic or synergistic effects between Cd and B[a]P at the prospected doses after 24 h of exposure. The results indicate that co-exposure to Cd and B[a]P may enhance toxicity by impairing specific responses and not through cumulative damage. - The interaction between cadmium and benzo[a]pyrene impairs specific responses to toxicity and tissue repair mechanisms.

  11. Benzo-α-pyrene induced oxidative stress in Caenorhabditis elegans and the potential involvements of microRNA.

    Science.gov (United States)

    Wu, Hongmei; Huang, Chenping; Taki, Faten A; Zhang, Yanqiong; Dobbins, Dorothy L; Li, Lin; Yan, Hongtao; Pan, Xiaoping

    2015-11-01

    In the present study oxidative stress induced by Benzo-α-pyrene (BaP) exposure and the potential involvements of microRNA were investigated. The Caenorhabditis elegans (C. elegans) was applied as model organism. The C. elegans at L1-stage were randomly divided into 4 groups and exposed to 0, 0.2, 2.0, and 20μM BaP for 30h. Expressions of SKiNhead-1 (SKN-1), gamma-glutamine cysteine synthase heavy chain (GCS-1), and their potential regulatory factors in insulin/IGF-1/FOXO signaling pathway and the p38 MAPK pathway were analyzed. The expressions of potentially involved microRNAs were investigated as well. Results demonstrated that expressions of SKN-1 and GCS-1 were altered significantly following BaP exposure (Pelegans. The expressional response of GCS-1 to BaP exposure might be independent of the regulation of SKN-1 in C. elegans. The microRNAs might be involved in the regulations of SKN-1 and GCS-1 expression following BaP exposure in C. elegans.

  12. Electron-accepting potential of solvents determines photolysis rates of polycyclic aromatic hydrocarbons: Experimental and density functional theory study

    International Nuclear Information System (INIS)

    Photochemical behaviour of polycyclic aromatic hydrocarbons (PAHs) is strongly dependent on the physical and chemical nature of the media in/on which they exist. To understand the media effects, the photolysis of phenanthrene (PHE) and benzo[a]pyrene (BaP) in several solvents was investigated. Distinct photolysis rate constants for PHE and BaP in the different solvents were observed. Some theoretical parameters reflecting the solvent properties were computed and employed to explain the solvent effects. Acetone competitively absorbed light with PHE and BaP, and the excited acetone molecules played different roles for the photodegradation of PHE and BaP. The photolysis rate constants of PHE and BaP in hexane, isopropanol, ethanol, methanol, acetonitrile and dichloromethane were observed to correlate with the electron-accepting potential of the solvent molecules. Absolute electronegativity of the solvents linearly correlated with the photolytic activity (log k) of the PAHs significantly. The results are important for better understanding the photodegradation mechanism of PAHs in different media.

  13. The combined toxicity of dibutyl phthalate and benzo(a)pyrene on the reproductive system of male Sprague Dawley rats in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Chen Xuemei [Laboratory of Reproductive Biology, Chongqing Medical University, Chongqing 400016 (China); An Hui; Ao Lin; Sun Lei; Liu Wenbin; Zhou Ziyuan [Department of Hygenic Toxicology, College of Military Preventive Medicine, Key Lab of Medical Protection for Electromagnetic Radiation, Ministry of Education of China, Third Military Medical University, Chongqing 400038 (China); Wang Yingxiong, E-mail: wyx61221@yahoo.com.cn [Laboratory of Reproductive Biology, Chongqing Medical University, Chongqing 400016 (China); Cao Jia, E-mail: caojia1962@126.com [Department of Hygenic Toxicology, College of Military Preventive Medicine, Key Lab of Medical Protection for Electromagnetic Radiation, Ministry of Education of China, Third Military Medical University, Chongqing 400038 (China)

    2011-02-15

    Our previous studies revealed more than 100 pollutants, most of which were endocrine disruptors (EDs) in two Chinese rivers, the Jialing and the Yangtze near Chongqing. Most EDs, such as dibutyl phthalate (DBP) and benzo(a)pyrene (BaP), are known to act individually as reproductive toxicants. However, little is known about the combined toxicity of DBP and BaP. In the current study, male Sprague Dawley rats were subchronically exposed to single doses of DBP (250 mg/kg), single doses of BaP (5 mg/kg) and combined doses of DBP and BaP. Significant adverse effects were observed on the reproductive system, including decreased sperm count, increased production of abnormal sperm, changes in serum testosterone levels and irregular arrangements of the seminiferous epithelium. Biochemical analyses showed that the activities of superoxide dismutase and glutathione peroxidase decreased after exposure to these EDs. Therefore, our data suggest that exposure to DBP and BaP, in either separate or combined doses, can affect the reproductive system of male rats adversely via oxidative stress-related mechanisms. No significant additive effect was observed after combined exposure. These results indicate that exposure to mixtures of EDs have unexpected and elusive effects. Our findings provide preliminary but important data for assessing water safety in China.

  14. Selecting reference concentrations for fish biliary PAH metabolites for stream ecological exposure assessment

    International Nuclear Information System (INIS)

    Reference concentrations for two types of biliary PAH metabolites were empirically determined for two species of fish. Bile from white sucker and common carp were collected from streams in Ohio having poor to excellent condition based on a modified index of biotic integrity (MIBI). PAH were measured using fixed wavelength fluorescence with excitation/emission pairs at 290/335 nm for naphthalene-type (NAPH) and 380/430 nm for benzo[a]pyrene-type (B[a]P) metabolites. Exposures to PAH were estimated for petroleum sources with NAPH and from combustion sources with B[a]P. Three criteria were used to select reference concentrations. Site criteria were: an MIBI score of at least 30, relatively low median values of biliary PAH concentration, and low statistical variation. The median reference values selected were 0.5 microg B[a]P/mg protein and 50 pg NAPH/mg protein for white sucker and 0.5 microg B[a]P/mg protein and 90 microg NAPH/mg protein for common carp. Of the 28 sites sampled with white sucker in 1993, 68% exceeded the reference value for NAPH and 32% for B[a]P. Of the 22 sites sampled with carp, 68% exceeded the value for NAPH and 41% for B[a]P. Likewise, more sites with MIBI scores between 30 and 60 exceeded the NAPH reference than the B[a]P reference concentration

  15. Selecting reference concentrations for fish biliary PAH metabolites for stream ecological exposure assessment

    International Nuclear Information System (INIS)

    Reference concentrations for two types of biliary polycyclic aromatic hydrocarbon (PAH) metabolites were empirically determined for two species of fish. Bile from white sucker and common carp were collected from streams in Ohio having poor to excellent conditions based on a modified index of biotic integrity (mIBI). PAH were measured using fixed wavelength fluorescence with excitation/emission pairs at 290/335 nm for naphthalene-type (NAPH) and 380/430 nm for benzo[a]pyrene-type (B[a]P) metabolites. Exposures to PAH's were estimated for petroleum sources with NAPH and from combustion sources with B[a]P. Three criteria were used to select reference concentrations. Site criteria were: an mIBI score of at least 30; relatively low median values of biliary PAH concentration; and low statistical variation. The median reference values selected were 0.5 microg B[a]P/mg protein and 50 microg NAPH/mg protein for white suckers and 0.5 microg B[a]P/mg protein and 90 microg NAPH/mg protein for common carp. Of the 28 sites sampled with white sucker in 1993, 68% exceeded the reference value for NAPH and 32% for B[a]P. Of the 22 sites sampled with carp, 68% exceeded the value for NAPH and 41% for B[a]P. Likewise, more sites with mIBI scores between 30 and 60 exceeded the NAPH reference than the B[a]P reference concentration

  16. IN VITRO PRODUCTION OF MICRORHIZOMES IN GINGER (ZINGIBER OFFICINALE ROSCO

    Directory of Open Access Journals (Sweden)

    Mohamed Abbas

    2014-10-01

    Full Text Available The present investigation was carried out to highlight an effective protocol for in vitro production of ginger microrhizomes. Microrhizomes were induced at the base of the in vitro derived shoots upon transfer to MS medium containing various concentrations of (30, 60 and 90 g/L, BAP: 6-benzylaminopurine (3, 6 and 9 mg/L and grown under varying photoperiodism in addition to the MS medium supplemented with 9 mg/L BAP and 60-90 g/L sucrose under 16-h photoperiod within 10 weeks of cultivation were the best conditions for microrhizomes induction. Ginger microrhizomes formation in vitro was found to be controlled by many factors, including the concentrations of BAP and sucrose as well as photoperiodism during culturing period.

  17. Transport of benzo[alpha]pyrene in the dually perfused human placenta perfusion model: effect of albumin in the perfusion medium

    DEFF Research Database (Denmark)

    Mathiesen, Line; Rytting, Erik; Mose, Tina;

    2009-01-01

    Transport of benzo[alpha]pyrene (BaP) across the placenta was examined because it is a ubiquitous and highly carcinogenic substance found in tobacco smoke, polluted air and certain foods. Foetal exposure to this substance is highly relevant but is difficult to estimate. The human placenta is unique...... compared to other species; since it is available without major ethical obstacles, we have used the human placenta perfusion model to study transport from mother to foetus. Placentas were donated after births at Rigshospitalet in Copenhagen from pregnant mothers who signed an informed consent. Ba......P is lipophilic and studies using cell culture medium in 6-hr placenta perfusions showed minimal transport through the placenta. To increase the solubility of BaP in perfusion medium and to increase physiological relevance, perfusions were also performed with albumin added to the perfusion medium [2 and 30 mg...

  18. TLR2 Controls Intestinal Carcinogen Detoxication by CYP1A1

    DEFF Research Database (Denmark)

    Do, Khoa; Fink, Lisbeth Nielsen; Jensen, Thomas Elbenhardt;

    2012-01-01

    cells and enterocytes. Here we report that intestinal CYP1A1 is silenced in TLR2-deficient mice, even when under exposure to the carcinogenic PAH benzo[a]pyrene (BaP). In contrast, hepatic CYP1A1 was moderately induced in TLR2-deficient mice without restoring their ability to clear BaP from systemic...... circulation, as present in wild-type animals. After feeding of BaP for 21 days, only TLR2(-/-) mice, but not their wild type littermates developed polyps in the colon. Gene expressions and protein concentrations of AHR and ARNT in the intestine did not differ between the genotypes. In conclusion, the presence...

  19. EROD activities of liver in Mugil so-iuy exposed to benzo(a) pyrene, pyrene and their mixture

    Institute of Scientific and Technical Information of China (English)

    Wang Chonggang; Chen Jixin; Yu Ang; Zheng Weiyun

    2003-01-01

    The effects on hepatic EROD (7-ethoxyresorufin O-deethylase) in Mugil so-iuy exposed to benzo(a)pyrene (BaP), pyrene and their mixtures of equal concentration were investigated, at concentrations of 0.1, 1.0, 5.0, 10.0, 50.0 μg/dm3, in experimental condition. Time-effects and doseresponse of the biochemical indexs were observed. The results showed that the hepatic EROD activities were induced by the exposure of BaP, pyrene and their mixtures at high concentration. Dose-response connections were that the hepatic EROD activities were elevated with increasing concentration of the pollutants. The combined effect of BaP and pyrene at 1:1 concentration ratio on hepatic EROD activity was antagonism.

  20. Observations of the effect of atmospheric processes on the genotoxic potency of airborne particulate matter

    DEFF Research Database (Denmark)

    Feilberg, Anders; Nielsen, Torben; Binderup, Mona-Lise;

    2002-01-01

    In this study, the relationship between genotoxic potency and the occurrence of polycyclic aromatic hydrocarbons (PAH), including benzo(a)pyrene (BaP), and nitro-PAH in urban and semi-rural air masses has been investigated. The Salmonella/microsome assay has been used as a measure of genotoxic...... potency. We find that the ratios of BaP/ mutagenicity and PAH/mutagenicity are highly variable. The processes responsible for the variation are formation and degradation of mutagens and transport of polluted air masses from heavily industrialized regions, Air masses from Central Europe are shown...... to be highly enriched in mutagens as well as in PAH and nitro-PAH. However, the mutagenic activity is much more elevated than the PAH levels when these air masses are mixed with local urban air. Part of the variation in the PAH/mutagenicity ratio can be explained by photochemical transformation. Since BaP has...

  1. Associations of total, dairy, and meat protein with markers for bone turnover in healthy, prepubertal boys

    DEFF Research Database (Denmark)

    Budek, Alicja Zofia; Hoppe, Camilla; Michaelsen, Kim Fleischer;

    2007-01-01

    , dairy, and meat protein intake with markers for bone turnover and sIGF-I in prepubertal, healthy boys (n ¼ 81). We measured bone turnover (enzyme-linked immunoassay) in serum osteocalcin (sOC), bone-specific alkaline phosphatase (sBAP), and C-terminal telopeptide of collagen type-I (sCTX); dietary...... with sBAP and sCTX. Further analyses showed that dairy protein decreased (P ¼ 0.05) sOC at a high meat protein intake (.0.8 g/kg), whereas meat protein increased (P ¼ 0.03) sOC at a low dairy protein intake (,0.4 g/kg). Total and meat protein intake was positively associated with sBAP (P # 0...

  2. Molecular Characterization of Melanoma Cases in Denmark Suspected of Genetic Predisposition

    DEFF Research Database (Denmark)

    Wadt, Karin A. W.; Aoude, Lauren G.; Krogh, Lotte;

    2015-01-01

    Both environmental and host factors influence risk of cutaneousmelanoma (CM), and worldwide, the incidence varies depending on constitutional determinants of skin type and pigmentation, latitude, and patterns of sun exposure. We performed genetic analysis of CDKN2A, CDK4, BAP1, MC1R, and MITFp.E318......K in Danish high-risk melanoma cases and found CDKN2A germline mutations in 11.3% of CM families with three or more affected individuals, including four previously undescribed mutations. Rare mutations were also seen in CDK4 and BAP1, while MC1R variants were common, occurring at more than twice...... cases of CM. In addition, we recommend that testing of BAP1 should not be conducted routinely in CM families but should be reserved for families with CM and uveal melanoma, or mesothelioma....

  3. Micropropagation of an Eucalyptus hybrid (Eucalyptus benthamii x Eucalyptus dunnii=Micropropagação de um híbrido de Eucalyptus (Eucalyptus benthamii x Eucalyptus dunnii.

    Directory of Open Access Journals (Sweden)

    Fabricio Augusto Hansel

    2011-10-01

    Full Text Available This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP and naphthaleneacetic acid (NAA for bud proliferation, and the ratio between BAP and gibberellic acid (GA3 in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1 of chlorine. Explants were grown on ½MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L-1 and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L-1 for bud production. They were elongated on MS and ½MS media supplemented with BAP (0, 0.05 and 0.10 mg L-1 and GA3 (0, 0.1, 0.2 and0.3 mg L-1. The 0.50 mg L-1 BAP and 0.050 mg L-1 NAA combination was optimal for bud proliferation for H12 and H20. GA3 concentrations of 0.10 and 0.20 mg L-1 combined with 0.10 mg L-1 BAP on ½MS resulted in the longest shoots, for H12 and H20, respectively. Regardless of clone, the rooting rate was low, with an average of 12.0% and 14.4% of plants having roots for in vitro and ex vitro conditions, respectively.Objetivou-se micropropagar E. benthamii x E. dunnii, testando concentrações de cloro para a assepsia de explantes, a concentração ótima de benzilaminopurina (BAP e ácido naftalenoacético (ANA para a proliferação de gemas e a relação entre BAP e ácido giberélico (GA3 em dois meios de cultura para o alongamento de brotações. Segmentos nodais dos genótipos H12, H19 e H20 foram desinfetados com 0,5; 1,0; 1,5 e 2,0% (v v-1 de cloro. Os explantes foram multiplicados em meio ½MS suplementado com BAP (0; 0,25; 0,50; 0,75 e 1,00 mg L-1 e ANA (0; 0,025; 0,050; 0,075 e 0,100 mg L-1 para produção de gemas, e alongados nos meios MS e ½MS suplementados com BAP (0; 0,05 e 0,10 mg L-1 e GA3 (0; 0,1; 0,2 e 0,3 mg L-1. A combinação de 0,50 mg L-1 de BAP e 0,050 mg L-1 de ANA proporcionou melhor proliferação de gemas para os gen

  4. Comparative assessment of national bioenergy strategies and biomass action plans in 12 EU countries. European Best Practice Report. Extended version

    International Nuclear Information System (INIS)

    This report is a key output of the EU project 'BAP Driver', an initiative of energy agencies from 8 European key bioenergy nations and the European Biomass Association (AEBIOM). The BAP Driver project aims at identifying ways for improvement of current national policy frameworks for bioenergy in Europe, and at leveraging the process of developing country-specific Biomass Action Plans (BAP). From a strategic perspective, the general approach of this report focuses on four stages, required for setting up national biomass strategies and action plans: Assessment of national biomass resources; Formulation of national bioenergy strategies and biomass action plans; Implementation of national bioenergy policies; Monitoring of national bioenergy markets and policies. Overall the analysis is split into three chapters corresponding to the following logical steps: Chapter B: Country analysis (12 individual country profiles); Chapter C: Benchmark analysis (comparative assessment of 12 countries); Chapter D: Best practice analysis (transnational conclusions across national boundaries)

  5. Comparative assessment of national bioenergy strategies and biomass action plans in 12 EU countries. European Best Practice Report. Executive Summary

    International Nuclear Information System (INIS)

    This report is a key output of the EU project 'BAP Driver', an initiative of energy agencies from 8 European key bioenergy nations and the European Biomass Association (AEBIOM). The BAP Driver project aims at identifying ways for improvement of current national policy frameworks for bioenergy in Europe, and at leveraging the process of developing country-specific Biomass Action Plans (BAP). From a strategic perspective, the general approach of this report focuses on four stages, required for setting up national biomass strategies and action plans: Assessment of national biomass resources; Formulation of national bioenergy strategies and biomass action plans; Implementation of national bioenergy policies; Monitoring of national bioenergy markets and policies. Overall the analysis is split into three chapters corresponding to the following logical steps: Chapter B: Country analysis (12 individual country profiles); Chapter C: Benchmark analysis (comparative assessment of 12 countries); Chapter D: Best practice analysis (transnational conclusions across national boundaries)

  6. Benzo(apireno em óleos de milho produzidos e comercializados no Brasil

    Directory of Open Access Journals (Sweden)

    TOLEDO M. Cecilia de F.

    1998-01-01

    Full Text Available Óleos de milho de diferentes marcas, produzidos e comercializados no Brasil, foram analisados quanto à presença de benzo(apireno (B(aP. A metodologia utilizada envolveu extração com ciclohexano, purificação em coluna de silica gel e determinação por cromatografia líquida de alta eficiência com detector de fluorescência. Todas amostras analisadas mostraram-se contaminadas por B(aP e os níveis determinados variaram entre as diferentes marcas analisadas e entre diferentes lotes de mesma marca, dentro da faixa de 0,85 a 25,17 µg/kg. Entre 49 amostras analisadas no período de dois anos, apenas uma apresentou nível de contaminação por B(aP abaixo do limite referencial de 1 µg/kg.

  7. Benzo(apireno, carbamato de etila e metanol em cachaças

    Directory of Open Access Journals (Sweden)

    Miriam Solange Fernandes Caruso

    2010-01-01

    Full Text Available The objective of this work was to evaluate the presence of benzo(apyrene (BaP, ethyl carbamate (EC and methanol in 61 samples of cachaça. The quantification of BaP was carried out using HPLC with fluorescence detection, EC concentrations was determined by GC/MS and that of methanol, by GC/FID. In all samples, the concentration of methanol remained below 5 mg 100 mL-1 absolute alcohol. The results of BaP varied from <0.03 to 0.86 μg L-1; the values of EC exceeded the limit established by Brazilian legislation (150 μg L-1 in 53% of the samples.

  8. ESTABLECIMIENTO DEL SISTEMA DE REGENERACION POR EMBRIOGÉNESIS SOMÁTICA DE Azadirachta indica A. Juss.

    Directory of Open Access Journals (Sweden)

    Maria D. Artigas R

    2014-09-01

    Full Text Available Azadirachta indica, es una planta con múltiples aplicaciones tanto forestal como farmacológica. Por ende, el establecimiento del sistema de cultivo in vitro por embriogénesis somática ofrece diversas y variadas ventajas, tales como obtener plantas altamente productivas en metabolitos. En este estudio, se utilizaron secciones foliares y cotiledonares, inducidas en medios MS (1962 suplementados con: BAP sólo y combinado con ANA/2,4-D, TDZ sólo y con ABA. La regeneración fue con MS sólo o con K + AIA y BAP + AIA. Como resultado se estableció un sistema eficiente con secciones de cotiledones, observándose organogénesis a bajas concentraciones de BAP, mientras a altos niveles de BAP (2,5 mg.L-1, así como  con TDZ + ABA (0,02 + 1mg.L-1 respectivamente favorecieron la embriogénesis somática primaria y secundaria en un 96 % y 71 % respectivamente. La regeneración fue 71 % con MS, mientras que el enraizamiento fue de 86,67 % con MS½, obteniéndose plantas completas a corto plazo. ABSTRACTAzadirachta indica, is a plant with multiple forest and pharmacological application. Therefore, the establishment of in vitro culture system for somatic embryogenesis offers several distinct advantages such as obtaining highly productive plant metabolites. In this study, were used sections cotyledon and leaf, induced on MS medium (1962 supplemented with: BAP alone and combined with NAA / 2,4-D, TDZ alone and ABA. Regeneration was with MS alone or with K + BAP + IAA and IAA. As a result was established an efficient system with cotyledon sections, being observed organogenesis at low concentrations of BAP, while high levels of BAP (2.5 mg.L-1 with 96 % and TDZ + ABA (0.02 + 1mg.L-1 with 71 %, favoring the primary and secondary somatic embryogenesis. Regeneration was 71 % with MS; rooting was 86.67 % with MS½, presenting whole plants obtained short term.RESUMOAzadirachta indica, é uma planta com várias aplicaçãos florestas e farmacológicas. Por

  9. Myeloperoxidase - 463A variant reduces benzo(a)pyrene diol epoxide DNA adducts in skin of coal tar treated patients

    Energy Technology Data Exchange (ETDEWEB)

    Rojas, M.; Godschalk, R.; Alexandrov, K.; Cascorbi, I.; Kriek, E.; Ostertag, J.; Van Schooten, F.J.; Bartsch, H. [German Cancer Research Center, Heidelberg (Germany). Div. of Toxicology & Cancer Risk Factors

    2001-07-01

    The skin of atopic dermatitis patients provides an excellent model to study the role of inflammation in benzo(a)pyrene (BaP) activation, since these individuals are often topically treated with ointments containing high concentrations of BaP. The authors determined, by HPLC with fluorescence detection, the BaP diol epoxide (BPDE)-DNA adduct levels in human skin after topical treatment with coal tar and their modulation by the -453G into A myeloperoxidase (MPO) polymorphism, which reduces MPO mRNA expression. The data show for the first time: (i) the in vivo formation of BPDE-DNA adducts in human skin treated with coal tar; (ii) that the MPO-463AA/AG genotype reduced BPDE-DNA adduct levels in human skin.

  10. Development of an efficient regeneration protocol for three genotypes of brassica juncea

    International Nuclear Information System (INIS)

    Two phytohormones, auxins (Naphthalene acetic acid and Indole acetic acid) and cytokinins (Benzyl aminopurine and Kinetin) with concentrations were used to develop an efficient regeneration protocol for 3 genotypes of Brassica juncea (UCD-635, RL-18 and NIFA RAYE). The explants were cultured on MS-medium supplemented with BAP 1.0 mgL/sup -1//NAA 0.1 mgL-1, BAP 2.0 mg L/sup -1//NAA 0.2 mg L/sup -1/, BAP 3.0 mgL/sup -1/ NAA 0.3 mg L-1 and Kinetin 1.0 mg L/sup -1/ IAA 0.1 mg L/sup -1/, Kinetin 2.0 mg L/sup -1//IAA 0.2 mg L/sup -1/, Kinetin 3.0mg L-1/IAA 0.3 mg L/sup -1/. Maximum callus production (65.55) was observed on MS medium containing with BAP 2.0 mgL-1/NAA 0.2 mg L/sup -1/. Maximum shooting (22.31) was observed BAP 3.0 mg L/sup -1//NAA 0.3 mg L/sup -1/ and KIN 3.0 mg L-1/IAA 0.3 mg L/sup -1/. Regeneration efficiency was found maximum (7.13) with BAP 3.0 mg L/sup -1//NAA 0.3 mg L/sup -1/. The three genotypes were found significantly different at p greater or equal to 0.05 in shoots production and regeneration efficiency. (author)

  11. Micropropagation of Costus speciosus (Koen. Sm. Using Nodal Segment Culture

    Directory of Open Access Journals (Sweden)

    Kshetrimayum PUNYARANI

    2010-03-01

    Full Text Available Nodal segments of Costus speciosus (Koen. Sm. containing single axillary buds were cultured on Murashige and Skoog medium (MS medium supplemented with plant growth regulators for inducing plantlets. For breaking of axillary bud dormancy, nodal segments were cultured on 40-70gl-1 sucrose or 1-13 �M adenine sulphate (AdS supplemented MS basal medium containing 5 �M 6-benzylaminopurine (BAP and 1�M ?-naphthalene acetic acid (NAA. The nodal segments cultured on 1-13 �M AdS, 5 �M BAP, 1 �M NAA and 50gl-1 sucrose showed simultaneous production of shoots and roots while those cultured on 5 �M BAP, 1 �M NAA and 40-70gl-1 sucrose produced shoots only. The most effective media for breaking axillary bud dormancy was 5 �M BAP, 1 �M NAA, 50 gl-1 sucrose and 10 �M AdS supplemented medium. The propagules from 40-70gl-1 sucrose produced roots in shoot multiplication medium, i.e.,10 �M AdS, 1 �M NAA, 50gl-1 sucrose and 3-11 �M BAP supplemented medium. The best response for shoot multiplication was on 10 �M AdS, 1 �M NAA, 50gl-1 sucrose and 7 �M BAP. The well-rooted shoots were hardened and transferred to the soil where they showed 95% survival rate. Results show that axillary bud can be used for micropropagation of Costus speciosus.

  12. Comparative Analysis of Serum (Antioxidative Status Parаmeters in Healthy Persons

    Directory of Open Access Journals (Sweden)

    Tatjana Ruskovska

    2013-03-01

    Full Text Available Five antioxidant and two oxidative stress assays were applied to serum samples of 43 healthy males. The antioxidant tests showed different inter-assay correlations. A very good correlation of 0.807 was observed between the ferric reducing ability of plasma (FRAP and total antioxidant status (TAS assay and also a fair correlation of 0.501 between the biological antioxidant potential (BAP and TAS assay. There was no statistically significant correlation between the BAP and FRAP assay. The anti-oxidant assays have a high correlation with uric acid, especially the TAS (0.922 and FRAP assay (0.869. The BAP assay has a much lower and no statistically significant correlation with uric acid (0.302, which makes BAP more suitable for the antioxidant status. The total thiol assay showed no statistically significant correlation with uric acid (0.114. The total thiol assay, which is based on a completely different principle, showed a good and statistically significant correlation with the BAP assay (0.510 and also to the TAS assay, but to a lower and not significant extent (0.279 and not with the FRAP assay (−0.008. The oxy-adsorbent test (OXY assay has no correlation with any of the other assays tested. The oxidative stress assays, reactive oxygen metabolites (ROM and total oxidant status (TOS, based on a different principle, do not show a statistically significant correlation with the serum samples in this study. Both assays showed a negative, but not significant, correlation with the antioxidant assays. In conclusion, the ROM, TOS, BAP and TTP assays are based on different principles and will have an additional value when a combination of these assays will be applied in large-scale population studies.

  13. Combined effect of tributyltin and benzo[a] pyrene on the levels of sex hormone and vitellogenin in female Sebastiscus marmoratus

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Tributyltin(TBT), an organometal used as an antifouling biocide, has been reported to induce masculinization of fish. Benzo[a]pyrene (BaP), a widespread carcinogenic polycyclic aromatic hydrocarbon, has been reported that its microsomal metabolites can produce an estrogenic response when tested in vitro. This study was therefore designed to examine the potential in vivo influence of TBT, BaP and their mixture on sex hormone levels in serum of Sebastiscus marmoratus, which were given 2 separate intraperitoneally(ip) injections(a single injection every 7 d) of TBT(0.5, 1, 5 and 10 mg/kg), BaP(0.5, 1, 5 and 10 mg/kg), or both in combination(0.5,1, 5 and 10 mg/kg); control fish received olive oil vehicle only. Six days after the 2nd injection, serum samples were collected and analyzed for sex hormone levels and alkali labile protein phosphorus (ALPP), which is related to the yolk precursor protein vitellogenin. The pollutants at all doses significantly reduced serum testosterone, estradiol and ALPP content after 2 injections compared with the corresponding controls. The reduction of the estradiol levels should be response for the decrease of the vitellogenin levels. The results in the present study suggested that aromatase seems not the major target acted by TBT and BaP in fish. This study demonstrated that TBT or BaP exposure both inhibit the reproductive potential in female Sebastiscus marmoratus. Combined effect of TBT and BaP on the serum testosterone, estradiol and ALPP was not antagonism from the anticipation.

  14. Detection and analysis on bone alkaline phosphatase activity of 2540 infants%2540例婴幼儿骨源性碱性磷酸酶活性检测分析

    Institute of Scientific and Technical Information of China (English)

    盛巍

    2012-01-01

    目的:调查长兴地区0~5岁婴幼儿骨健康状况.方法:使用骨源性碱性磷酸酶(BAP)试剂盒对2009~2010年来长兴妇幼保健院门诊就诊的2 540例0~5岁婴幼儿进行检测.结果:在2 540例0~5岁婴幼儿中,BAP活性正常的1 215例,占总人数的47.83%;BAP活性处于预防水平的1 196例,占总人数的47.09%;BAP活性处于治疗水平的129例,占总人数的5.08%.结论:长兴地区约50.00%的婴幼儿骨骼处于亚健康状态,易患佝偻病,需及早预防.%Objective: To investigate the bone health status of 0 - 5 - year - old infants in Changxing county. Methods: Bone alkaline phosphatase ( BAP) activity of 2 540 infants aged 0-5 years who went to outpatient department of the hospital from 2009 to 2010 was detected by BAP kit. Results; Among2 540 infante aged 0 -5 years old, I 215 infants were found with normal BAP activity, accounting for 47. 83% ; BAP activity of 1 196 infants was at prevention level, accounting for 47.09% ; BAP activity of 129 infants was at treatment level, accounting for 5.08%. Conclusion: The bones of about 50.00% of the infants in Changxing county were subhealthy, the infants are susceptible to rickets, and prevention should be earned out early.

  15. Dose-related carcinogenic effects of water-borne benzo(a)pyrene on livers of two small fish species

    Energy Technology Data Exchange (ETDEWEB)

    Hawkins, W.E.; Walker, W.W.; Overstreet, R.M.; Lytle, T.F.; Lytle, J.S.

    1988-12-01

    Benzo(a)pyrene (BaP) administered by water-borne exposures caused dose-related carcinogenic effects in livers of two small fish species, the Japanese medaka (Oryzias latipes) and the guppy (Poecilia reticulata). Medaka and guppies each were given two 6-h exposures. The first exposure was conducted on 6- to 10-day-old specimens. The second exposure was given 7 days later. The tests incorporated five treatment groups: (1) control, (2) carrier (dimethylformamide) control, (3) low BaP dose (not detectable--4 ppb), (4) intermediate BaP dose (about 8-47 ppb BaP), and (5) high BaP dose (200-270 ppb). Following the high-dose exposure, hepatocellular lesions classified as foci of cellular alteration (altered foci), adenomas, and hepatocellular carcinomas occurred in both species. In medaka, the lesions appeared to develop sequentially with the appearance of altered foci followed by adenomas and then hepatocellular carcinomas. Most lesions in guppies, however, were classified as altered foci although a few adenomas occurred in the early (24-week) sample and hepatocellular carcinomas occurred in the late (52-week) sample. When total lesions were combined, medaka had an 11% incidence at 24 weeks after the initial exposure and 36% incidence at 36 weeks. In guppies, 8% had liver lesions at 24 weeks, 19% at 36 weeks, and 20% at 52 weeks. A single extrahepatic neoplasm, a capillary hemangioma in a gill filament, occurred in a medaka from the 36-week high-dose sample. The results suggest that the medaka and guppy are capable of metabolizing water-borne BaP to carcinogenic metabolites which initiate hepatic tumor development.

  16. Research on the relationship between serum 25-hydroxyvitamin D and bone metabolism markers in children

    Institute of Scientific and Technical Information of China (English)

    Qing-Jun Meng; Qing Hua; Ting-Ting Dai

    2015-01-01

    Objective:To explore the relevance between serum 25-hydroxyvitamin D and bone metabolism markers in children.Methods:A total of 167 children who visited the Growth and Development Clinic due to growth retardation, dysphoria, night terrors, and hyperhidrosis from September, 2012 to September, 2013 were enrolled in the study. Serum 25-hydroxyvitamin D [25 (OH)VitD],BAP and OC levels were measured by enzyme-linked immune method (ELSIA), while IGF-Ⅰ was measured by chemiluminescence. The relevance of 25 (OH) VitD with BAP, OC and IGF-Ⅰ was analyzed.Results: Serum 25 (OH) VitD level was decreased gradually with increasing age. There were significant differences between infancy group and adolescence group. With increasing age, serum OC level increased gradually.With decreasing 25 (OH)VitD level, serum OC level increased gradually,serum IGF-Ⅰ level decreased gradually, whereas BAP had no significant change. 25 (OH)VitD level showed a negative positive linear correlation with OC, a positive linear correlation with IGF-Ⅰ, and no significant linear correlation with BAP in children.Conclusions:The level of 25 (OH)VitD, BAP, OC and IGF-Ⅰ vary in children with different ages. Adolescence and school-age children have severer vitamin D deficiency than infants. Vitamin D level may be correlated with BMD within a certain range. 25 (OH)VitD level showed a negative positive linear correlation with OC, a positive linear correlation with IGF-Ⅰ, and no significant linear correlation with BAP in children. Diagnosis of the body's VitD nutritional status by bone metabolism markers needs further study.

  17. Alterations of rat liver mitochondrial oxidative phosphorylation and calcium uptake by benzo[a]pyrene

    International Nuclear Information System (INIS)

    We report that oxidative phosphorylation and Ca2+ uptake processes are enhanced in liver mitochondria isolated from benzo[a]pyrene (B[a]P)-treated rats. The carcinogen did not affect either the respiratory control index or the Ca2+ control ratio. B[a]P treatment increased the oxidation rate of several substrates that donate electrons at the level of all three coupling sites, either the ADP- or Ca2+-stimulated rates or those observed after ADP or Ca2+ exhaustion. However, the efficiency of energy coupling was maintained because both ADP/O and Ca2+/site ratios remained unchanged. The electron flow through NADH-oxidase, NADH-duroquinone reductase, NADH-juglone reductase, NADH-cytochrome c reductase, succinate-cytochrome c reductase, and cytochrome c oxidase was enhanced by B[a]P; however, succinate dehydrogenase activity was not affected. All these effects depended on the time post B[a]P administration, with a greater increase close to 48 h after administration of the carcinogen. The contents of cytochromes b, c1, and a + a3 from liver mitochondria, especially those isolated 48 h after B[a]P, were also significantly increased, although cytochrome c levels was just lightly increased 24 h after B[a]P treatment. These results suggest that B[a]P treatment stimulates mitochondrial respiration by increasing the level of several components of the mitochondrial respiratory chain. This may reflect mitochondrial adaptation to the cellular energy requirements of cell division in the neoplastic transformation process

  18. The aryl hydrocarbon receptor nuclear translocator (Arnt) is required for tumor initiation by benzo[a]pyrene

    OpenAIRE

    Shi, Shengli; Yoon, Diana Y.; Hodge-Bell, Kimberly C.; Bebenek, Ilona G.; Whitekus, Michael J.; Zhang, Ruixue; Cochran, Alistair J.; Huerta-Yepez, Sara; Yim, Sun-Hee; Gonzalez, Frank J; Jaiswal, Anil K.; Hankinson, Oliver

    2009-01-01

    Benzo[a]pyrene (B[a]P) is a ligand for the aryl hydrocarbon receptor (Ahr). After binding ligand, Ahr dimerizes with the aryl hydrocarbon receptor nuclear translocator (Arnt) protein, and the dimer upregulates the transcription of Cyp1a1, Cyp1b1 and other enzymes involved in the metabolic activation of B[a]P. Arnt null mice die in utero. Mice in which Arnt deletion occurs constitutively in the epidermis die perinatally. In the current study, mice were developed in which the Arnt gene could be...

  19. Variation in genotoxic stress tolerance among frog populations exposed to UV and pollutant gradients

    International Nuclear Information System (INIS)

    Populations of widely distributed species can be subjected to unequal selection pressures, producing differences in rates of local adaptation. We report a laboratory experiment testing tolerance variation to UV-B and polycyclic aromatic hydrocarbons (PAHs) among common frog (Rana temporaria) populations according to their natural exposure level in the field. Studied populations were naturally distributed along two gradients, i.e. UV-B radiation with altitude and level of contamination by PAHs with the distance to emitting sources (road traffic). Tadpoles from eight populations were subjected to (1) no or high level of artificial UV-B; (2) four concentrations of benzo[a]pyrene (BaP) (0, 50, 250, 500 μg L-1); (3) simultaneously to UV-B and BaP. Since both stressors are genotoxic, the number of micronucleated erythrocytes (MNE) in circulating red blood cells was used as a bioindicator of tadpole sensitivity. High-altitude populations appear to be locally adapted to better resist UV-B genotoxicity, as they showed the lowest MNE numbers. Conversely, no correlation was observed between levels of PAH contamination in the field and tadpole tolerance to BaP in the laboratory, indicating the absence of local adaptation for BaP tolerance in these populations. Nevertheless, the decrease of MNE formation due to BaP exposure with altitude suggests that high-altitude populations were intrinsically more resistant to BaP genotoxicity. We propose the hypothesis of a co-tolerance between UV-B and BaP in high-altitude common frog populations: local adaptation to prevent and/or repair DNA damage induced by UV-B could also protect these highland populations against DNA damage induced by BaP. The results of this study highlight the role of local adaptation along pollutant gradients leading to tolerance variation, which implies that is it necessary to take into account the history of exposure of each population and the existence of co-tolerance that can hide toxic effects of a new

  20. ENDOCRINE DISRUPTIVE ACTIONS OF INHALED BENZO(a)PYRENE ON OVARIAN FUNCTION AND FETAL SURVIVAL IN FISHER F-344 ADULT RATS

    OpenAIRE

    Archibong, Anthony E.; Ramesh, Aramandla; Inyang, Frank; Niaz, Mohammad S.; Hood, Darryl B.; Kopsombut, Prapaporn

    2012-01-01

    This study evaluated the effect of inhaled BaP on female reproductive function. Rats were exposed to 50, or 75 or 100 μg BaP/m3, four hours a day for 14 days via inhalation. Plasma E2, P4, LH and FSH concentrations were determined. Ovarian BaP metabolism and aryl hydrocarbon hydrolase (AHH) activity at proestrus were determined and fertility evaluations were conducted. Ovulation rate and number of pups/litter were reduced in rats exposed to 100 μg BaP/m3 compared with other treatment and cont...

  1. Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene

    Energy Technology Data Exchange (ETDEWEB)

    Yuen, Bonny B.H. [Centre for Coastal Pollution and Conservation, Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong (China); Nicholas School of the Environment, Duke University, Durham, NC (United States); Wong, Chris K.C. [Department of Biology, Baptist University of Hong Kong, Waterloo Road, Kowloon, Hong Kong (China); Woo, N.Y.S. [Department of Biology, Chinese University of Hong Kong, New Territory, Hong Kong (China); Au, Doris W.T. [Centre for Coastal Pollution and Conservation, Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong (China)]. E-mail: bhdwtau@cityu.edu.hk

    2007-05-15

    The sublethal toxicity of dietary benzo[a]pyrene, B[a]P, on fish growth and intestinal morphofunctional changes [as measured by epithelial turnover, cell proliferation, hyperplasia, de novo crypt formation and protein absorption efficiency (i.e. expression of proton/peptide co-transporter, PepT-1, on the mucosal brush border)] were studied for the carnivorous orange-spotted grouper (Epinephelus coioides). Juvenile fish were force-fed daily with pellets containing environmentally realistic concentrations of B[a]P (dissolved in corn oil) at 0.25 {mu}g/g body weight (low-dose) and 12.5 {mu}g/g body weight (high-dose) for 4 weeks, followed by a control diet for a further 4 weeks to assess recovery. Although growth inhibition was observed in fish treated with high-dose B[a]P during the exposure period, no mortality was observed throughout the 8-week experiment. Significant hyperplasia of basal enterocytes of mucosal folds was detected shortly after 3-day exposure to the high-dose B[a]P. Moreover, a faster epithelial turnover was measured in the high-dose B[a]P exposed fish at exposure week 1, which was followed by an increase of basal cell proliferation and a reduction of PepT-1 expression at exposure week 2. The formation of de novo crypts, resemblance to the cancer predisposition syndrome 'juvenile polyposis', was significantly higher in the intestine of high-dose treated fish as compared to the control at exposure week 2 and onwards. Abnormal cytoplasmic extrusions were frequently observed in mucosal folds of high-dose fish at exposure week 4. In the low-dose treatment group, only the expression of PepT-1 was significantly reduced at exposure week 2 and an early adaptive response was observed at exposure week 4. Despite all these intestinal disturbances were reversible in fish upon the abatement to dietary B[a]P (within 1-4 weeks), environmental realistic levels of foodborne B[a]P could induce sublethal toxicity to E. coioides, and probably impose potential

  2. In vitro organogenesis in some citrus species

    Directory of Open Access Journals (Sweden)

    Evandro Henrique Schinor

    2011-06-01

    Full Text Available In vitro organogenesis of Citrus was studied for the genotypes Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP - 0.0; 0.5; 1.0; 1.5 or 2.0 mg L-1. For the recalcitrant genotypes C. limonia and C. aurantium the in vitro organogenesis was also studied with internodal segments-derived explants, cultured in MT salts and vitamins medium supplemented with 0; 0.5; 1.0; 2.0, or 4.0 mg L-1 of BAP. The efficiency of culture medium supplementation with the combination of BAP (0.0; 1.0, or 2.0 mg L-1 and NAA (1-naphthaleneacetic acid - 0.0; 0.3, or 0.5 mg L-1 in the development of adventitious shoots was evaluated for C. aurantium. Culture medium supplementation with BAP is not essential for the adventitious shoots development in the four genotypes studied when epicotyl segments-derived explants are used. In general, culture media supplementation with BAP decreased the percentage of responsive explants excepted for C. sinensis cv. 'Natal' and C. limonia when the concentrations of 1.5 and 2.0 mg/L were used. The presence of cytokinin, in concentrations up to 2 mg/L, stimulated the in vitro organogenesis when internodal segments-derived explants were used for C. limonia and C. aurantium. For C. aurantium no adventitious shoots developed in explants (internodal segments cultured in basal culture medium, without BAP supplementation. Although no statistic differences could be detected, culture media supplementation with the combination of BAP and NAA favored the development of adventitious shoots in C. aurantium. The best concentration of NAA varied according to BAP concentration. The results presented herein, show that Citrus in vitro organogenesis depends on the interaction of culture medium composition, explant differentiation level, and genotype.

  3. Regeneração in vitro de melão, cv. 'Gaúcho' In vitro regeneration of melon, cv. 'Gaúcho'

    Directory of Open Access Journals (Sweden)

    Daiane Schmidt de Pinho

    2010-05-01

    Full Text Available O objetivo do presente trabalho foi otimizar um protocolo de regeneração de explantes cotiledonares de melão, cultivar 'Gaúcho', avaliando a composição de meios de germinação e o tempo de permanência dos explantes nesses meios. Para isso, as sementes foram germinadas em meio MS semi-sólido contendo BAP ou ANA e sem esses reguladores de crescimento. As sementes permaneceram nesses meios por um, dois, três e quatro dias, sendo então seus cotilédones inoculados em meio MS contendo diferentes concentrações de BAP (0,5; 0,9; 1,5 e 2,0mg L-1. As maiores taxas de regeneração ocorreram nos cotilédones oriundos de sementes mantidas durante um e dois dias nos meios contendo 0,5 e 0,9mg L-1 de BAP. O aumento da permanência dos explantes nos meios de germinação e as elevadas concentrações de BAP nos meios de regeneração diminuíram a capacidade organogênica dos explantes e incrementaram a formação de calos.The aim of the present study was to optimize a cv. Gaucho melon cotyledon explant regeneration protocol, evaluating the germination media composition and the explant exposition period in these media. For this purpose, seeds were germinated in semi-solid MS medium containing either BAP or ANA, and without growth regulators. The seeds were kept in these media for one, two, three or four days; afterwards, their cotyledons were inoculated in MS medium containing different BAP concentrations (0.5; 0.9; 1.5 and 2.0mg L-1. The highest regeneration rates occurred with cotyledons from seeds which had been kept for one or two days in media containing 0.5 and 0.9mg L-1 BAP. Both the increase in the explant exposition time in germination media and high BAP concentrations in regeneration media decreased explant organogenic capacity, and increased callus formation.

  4. Deep sequencing of uveal melanoma identifies a recurrent mutation in PLCB4

    DEFF Research Database (Denmark)

    Johansson, Peter; Aoude, Lauren G; Wadt, Karin;

    2016-01-01

    Next generation sequencing of uveal melanoma (UM) samples has identified a number of recurrent oncogenic or loss-of-function mutations in key driver genes including: GNAQ, GNA11, EIF1AX, SF3B1 and BAP1. To search for additional driver mutations in this tumor type we carried out whole-genome or wh......Next generation sequencing of uveal melanoma (UM) samples has identified a number of recurrent oncogenic or loss-of-function mutations in key driver genes including: GNAQ, GNA11, EIF1AX, SF3B1 and BAP1. To search for additional driver mutations in this tumor type we carried out whole...

  5. Multiplicação in vitro de amoreira-preta cultivar Brazos In vitro multiplication of blackberry cv. Brazos

    Directory of Open Access Journals (Sweden)

    Fabiola Villa

    2006-04-01

    Full Text Available A micropropagação da amoreira-preta pode gerar plantas livres de vírus e em curto espaço de tempo. Com o objetivo de aprimorar técnicas de micropropagação de amoreira-preta cultivar Brazos (Rubus idaeus L., segmentos nodais, oriundos de plântulas preestabelecidas in vitro foram excisados e inoculados em meio WPM (0, 50, 100, 150 e 200%, suplementado com diferentes concentrações de BAP (0; 0,5; 1,0; 2,0 e 4,0 mg L-1. Após a inoculação, os explantes foram transferidos para sala de crescimento a 27±1ºC, irradiância de 35 mmol m² s¹ e fotoperíodo de 16 horas, onde permaneceram por 60 dias. O delineamento experimental utilizado foi inteiramente casualizado, utilizando-se de quatro repetições com quatro explantes cada. Maior número de brotos foi proporcionado com 1,0 mg L-1 de BAP associado a 100% de meio WPM e maior comprimento médio dos brotos após 60 dias foi verificado em 1,0 mg L-1 de BAP associado a 200% de meio WPM. Maior peso de matéria seca da parte aérea foi obtido em meio WPM 200% acrescido de 0,5 mg L-1 de BAP.With the objective of multiplying blackberry cv. Brazos, nodal segments, coming from in vitro plants previously selected, were excised and inoculated in WPM culture medium (0, 50, 100, 150 and 200%, supplemented with different concentrations of BAP (0; 0,5; 1,0; 2,0 and 4,0 mg L-1. After inoculation, the explants were transferred to culture room, at 27±1ºC temperature, 35 mmol m² s¹ ofirradiance and photoperiod of 16 hours, for 60 days. The experimental was a design randomized complete block, with four replications and four explants each. Greater number of sprouts was provided with 1,0 mg L-1 of BAP associated with 100% WPM culture medium and larger sprouts length average after 60 days were verified in 1,0 mg L-1 of BAP associated with 200% WPM culture medium. Higher dry matter weight of the aerial part was obtained in 200% WPM culture medium added with 0,5 mg L-1 of BAP.

  6. Antioxidant responses to benzo[a]pyrene,tributyltin and their mixture in the spleen of Sebasticus marmoratus

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    It has been repotted that there is an interaction between Benzo[a]pyrene (BaP),a widespread carcinogenic polycyclic aromatic hydrocarbon,and tributyltin (TBT),an organometal used as an antifouling biocide.This study was therefore designed to examine the potential in vivo influence of BaP,TBT and their mixture on splenic antioxidant defense systems of Sebastiscus marmoratus.The fish were exposed to water containing euvironmentally relevant concentrations of BaP,TBT and their mixture.Spleens were collected for biochemical analysis after exposure for 7,25,50 d and after recovery for 7,20 d.Cotreatment with BaP and TBT for 7 d potentiated the induction of glutathione pemxidase (GPx) activity by BaP or TBT alone.The cotreatment for 25 and 50 d resulted in inhibition of GPx activity,which was similar to the effect of TBT.Splenic glutathione S-transferase (GST) activities were significantly elevated in S.marmoratus exposed to BaP starting from 7d and remained high up to 25 d.However,no further activity change was found with prolonged exposure.Cotreatment of BaP and TBT primarily inhibited the GST activity,which was similar to the effect of TBT.Cotreatment with BaP and TBT for 25 or 50 d potentiated the depletion of GSH (glutathione) by BaP or TBT alone.MDA (malondialdehyde) contents in spleen of S.marmoratus were not significantly altered compared with the control during the test period.Spleen,as an immune organ,is sensitive to exposure of BaP or TBT.It should have an effective mechanism to counteract oxidative damage.Antioxidative defense systems in spleen of S.marmoratus should be considered as potential biomarkers.Short-term exposure of BaP or TBT could result in induction of antioxidant defense system.A significant decrease of these indices.such as GSH,GST,GPx might indicate more severe contamination.

  7. Adjuvant role of Pseudomonas flagellin for Acinetobacter baumannii biofilm associated protein

    Science.gov (United States)

    Sefidi, Mozhgan Derakhshan; Rasooli, Iraj; Owlia, Parviz; Talei, Daryush; Astaneh, Shakiba Darvish Alipour; Nazarian, Shahram

    2016-01-01

    AIM To study immunogenicity of Pseudomonas N terminal flagellin as an adjuvant for Acinetobacter baumannii (A. baumannii) biofilm associated protein (Bap). METHODS The N terminal flagellin gene was amplified. The pET28a (+) and polymerase chain reaction products were digested with HindIII and EcoR I. The ligation of N terminal flagellin into pET28a (‏+) was performed using T4 DNA ligase and was then transformed into Escherichia coli BL21 (DE3) as a suitable expression host. pET28a (‏+) vector harboring a conserved region of Bap from our previous work was used. The recombinant proteins were expressed, analyzed by SDS-PAGE method and was purified by affinity chromatography with His-Tag residues followed by confirmation with western blotting. Mice were immunized with recombinant N terminal flagellin and Bap subunits. The immunized animals were intranasally (i.n) challenged with A. baumannii and Pseudomonas aeruginosa (P. aeruginosa). RESULTS The flagellin enhanced the immunogenicity of Bap causing an increase in specific IgG titers in serum (P Bap-Flagellin immunized group challenged with A. baumannii showed significantly lower bacterial load compared to the control group. The bacterial loads were studied in internal organs. A. baumannii infected immunized animals with Bap-Flagellin exhibited internal organs with minor bacterial load while P. aeruginosa PAO1 infected group showed heavy bacterial load of (4.3 ± 0.12) × 106, (1.1 ± 0.01) × 106 and (2.2 ± 0.22) × 106 per gram of lungs, liver and spleen respectively. Bacterial loads were detected per gram of lungs, liver and spleen of the mice group immunized with Bap were (1.2 ± 0.06) × 107, (11.1 ± 0.041) × 105 and (3.6 ± 0.42) × 106 respectively. In vivo neutralization assay indicated that all experimental mice groups, except for Flagellin administered group was significantly (P < 0.05) protected against A. baumannii. CONCLUSION These results demonstrate that P. aeruginosa Flagellin as an adjuvant for

  8. Micropropagation of Plantago asiatica L. through culture of shoot-tips

    Directory of Open Access Journals (Sweden)

    Joanna Makowczyńska

    2011-04-01

    Full Text Available Shoot-tip multiplication of the medicinal species - Plantago asiatica was carried on MS medium with IAA and BAP or kinetin. Best results in micropropagation were achieved by adding 0.1 mg/dm3 IAA and 1 mg/dm3 BAP. After 6 weeks shoots were transferred to MS medium for rooting. The resulting plantlets were transferred after 8 weeks into pots and after a period of adaptation into the ground (field culture. The species Plantago asiatica was propagated in vitro by shoot-tip multiplication for the first time.

  9. Black tattoos protect against UVR-induced skin cancer in mice

    DEFF Research Database (Denmark)

    Lerche, Catharina M; Sepehri, Mitra; Serup, Jørgen;

    2015-01-01

    BACKGROUND: Black tattoos may involve risk of cancer owing to polycyclic aromatic hydrocarbons including benzo(a)pyrene (BaP) in inks. Ultraviolet radiation (UVR) induces skin cancer. The combination of UVR and black tattoo may therefore potentially be very problematic, but has not been previously...... studied. METHODS: Immunocompetent C3.Cg/TifBomTac mice (n = 99) were tattooed on the back with Starbrite Tribal Black(™) . This ink has a high content of the carcinogen BaP. Half of the mice were irradiated with three standard erythema doses UVR thrice weekly. Time to induction of first, second and third...

  10. Investigation on the Protective Effects of Cranberry Against the DNA Damage Induced by Benzo[a]pyrene

    Directory of Open Access Journals (Sweden)

    José A. Morales-González

    2012-04-01

    Full Text Available There are few reports that demonstrate the antigenotoxic potential of cranberries. Although the types of berry fruits consumed worldwide are many, this paper focuses on cranberries that are commonly consumed in Mexico (Vaccinium macrocarpon species. The purpose of the present study is to determine whether cranberry ethanolic extract (CEE can prevent the DNA damage produced by benzo[a]pyrene (B[a]P using an in vivo mouse peripheral blood micronucleus assay. The experimental groups were organized as follows: a negative control group (without treatment, a positive group treated with B[a]P (200 mg/kg, a group administered with 800 mg/kg of CEE, and three groups treated with B[a]P and CEE (200, 400, and 800 mg/kg respectively. The CEE and benzo[a]pyrene were administered orally for a week, on a daily basis. During this period the body weight, the feed intake, and the determination of antigenotoxic potential were quantified. At the end of this period, we continued with the same determinations for one week more (recovery period but anymore administration of the substances. The animals treated with B[a]P showed a weight increase after the first week of administration. The same phenomenon was observed in the lots combined with B[a]P and CEE (low and medium doses. The dose of 800 mg/kg of CEE showed similar values to the control group at the end of the treatment period. In the second part of the assay, when the substances were not administered, these experimental groups regained their normal weight. The dose of CEE (800 mg/kg was not genotoxic nor cytotoxic. On the contrary, the B[a]P increases the frequency of micronucleated normochromatic erythrocytes (MNNE and reduces the rate of polychromatic erythrocytes (PE at the end of the treatment period. With respect to the combined lots, a significant decrease in the MN rate was observed from the sixth to the eighth day of treatment with the two high doses applied; the highest protection (60% was obtained with

  11. Patient pools and the use of "patient means" are valuable tools in quality control illustrated by a bone-specific alkaline phosphatase assay

    DEFF Research Database (Denmark)

    Hinge, Maja; Lund, Erik D.; Brandslund, Ivan;

    2016-01-01

    BACKGROUND: Quality control (QC) is an essential part of clinical biochemistry to ensure that laboratory test results are reliable and correct. Those tests without a defined reference method constitute a special challenge, as is the case with bone-specific alkaline phosphatase (BAP). METHODS...... AND RESULTS: The present study reports an example where a shift in a BAP assay was detected by use of a patient pool and supported by a retrospective calculation of "patient mean", while the external QC and specific assay control material were unaffected by the shift. CONCLUSIONS: Patient pools and the use...

  12. Determination of the level of benzo[a]pyrene in fatty foods and food supplements

    OpenAIRE

    Van Der Wielen-Hustinx, Jacqueline Claire Agnes; Jansen, John; Martena, Martijn J.; De Groot, Henk; In T Veld, Paul

    2006-01-01

    Abstract A routine method was developed for the quantification of benzo[a]pyrene (BaP) in edible oils and food supplements. BaP is often taken as an indicator of the presence of polycyclic aromatic hydrocarbons. The method consists of on-line LC-clean up followed by injection to an HPLC-system connected with fluorescence detection. The method has good performance characteristics and gave good results in proficiency tests. From 2002 to 2004 about 1350 samples, oils and food supp...

  13. Regeneration of three sweet potato (Ipomea batatas (L.)) accessions via meristem, Nodal and callus induction

    International Nuclear Information System (INIS)

    In vitro regeneration of three sweet potato accessions UE007, UK-BNARI and SA-BNARI using meristem, nodal cuttings or callus induction was studied. Meristematic explants cultured on Murashige and Skoog (1962) basal medium supplemented with low concentration of benzylaminopurine (BAP) or kinetin resulted in callus with or without shoot development which delayed shoot emergence. The degree of callus development increased as the concentration of the cytokinin in the culture medium increased. Although, callus development was comparatively lower on kinetin amended medium than BAP amended medium, Murashige and Skoog medium supplemented with 0.25mg/1BAP had the highest shoot induction (80%). For further differentiation of callus or shoots into distinct stem and leaves, the culture were transferred into fresh MS medium supplemented with 0.25mg/1 BAP, 0.1 mg/1 NAA and 0.1 mg/1 Gibberellic acid (GA3. To overcome the delay in shoot initiation using meristem culture, nodal cuttings of sweet potato were used as explants and cultured on MS medium amended with 0.3 - 0.9mg/1 BAP. All explants cultured on 0.3 or 0.6mg/1 BAP developed shoots. Furthermore, liquid MS medium amended with 0.25mg/1 BAP, 0.1mg/I NAA, and 0.1mg/1 GA3 also enhanced early shoot development from nodal cutting explants compared to solid culture. Post flask acclimatisation of meristem or nodal cutting-derived plantlets showed that meristem derived plantlets were better acclimatised than nodal cutting plants due to vigorous root development leading to higher percentage survival in pots and subsequent tuber production. Callusogenesis was achieved when leaf lobe explants were cultured on CLC/ Ipomoea medium supplemented with 1.0 - 4.0mg/1 2,4-D with 4.0mg/1 2,4-D being the optimal concentration. However, the calli were non-embryogenic and therefore could not produce embryos when transferred to 0.1mg/1 BAP amended medium but rather produced either single or multiple shoots. The highest percentage shoot (83.3%) was

  14. The Effects of Benzo(A) Pyrene Doxorubicin and Paclitaxel on P170 Glycoprotein

    OpenAIRE

    COŞAN, Didem

    2001-01-01

    B(a)P is a mutagenic, carcinogenic and teratogenic substance. Paclitaxel and doxorubicin are antineoplastic drugs widely used in cancer treatment. The purpose of this study is to observe the effects of doxorubicin and paclitaxel on p170 glycoprotein in rat liver and kidney tissue after administration of B(a)P. As is well known, p170 glycoprotein is an indicator of drug resistance. We hypothesized that a combination of these antineoplastic drugs would cause lower p170 levels and thus would ha...

  15. Multilaboratory Validation of Rapid Spot Tests for Identification of Escherichia coli

    OpenAIRE

    York, Mary K.; Baron, Ellen Jo; Clarridge, Jill E.; Thomson, Richard B.; Weinstein, Melvin P.

    2000-01-01

    To validate the accuracy of rapid tests for identification of Escherichia coli, five laboratories sequentially collected 1,064 fresh, clinically significant strains with core criteria of indole-positive, oxidase-negative, nonspreading organisms on sheep blood agar plates (BAP), having typical gram-negative rod plate morphology, defined as good growth on gram-negative rod-selective media. An algorithm using beta-hemolysis on BAP, lactose reaction on eosin-methylene blue or MacConkey agar, l-py...

  16. Mikropropagasi Tunas Anggrek Hitam (Coelogyne pandurata Lindl) Dengan Pemberian Benzil Amino Purin dan Naftalen Asam Asetat

    OpenAIRE

    Lubis, Nanda Nurlela

    2010-01-01

    The aim of the research is to know the influence of Benzylamino purine (BAP) and Naphtalen-3-acetic acid (NAA) concentration on micropropagation black orchid buds. The research was carried out in the Tissue Culture laboratory, Department of Agronomy, Faculty at Agriculture North Sumatera University, Medan from January to March 2010. This research used Randomized Block Design with two factor. First factor was BAP concentration consist of four levels: 0 mg/l ; 1 mg/l; 2 mg/l dan 3 mg/l. The se...

  17. In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISH-B1 through enhanced axillary branching

    OpenAIRE

    Pati, Rajesh; Chandra, Ramesh; Chauhan, Ugam Kumari; Mishra, Maneesh; Srivastava, Navin

    2008-01-01

    Rapid clonal micropropagation protocol of Aegle marmelos (L.) Corr. cv. CISH-B1 was achieved by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10–15th nodal region of shoots during September gave quick in vitro bud burst (5.33 days) when cultured on MS medium supplemented with BAP, 8.84 μM + IAA 5.7 μM. The maximum number of proliferated shoots (9.0/explant) were obtained on same medium supplemented with BAP 8.84 μM + IAA 5.7 μM. T...

  18. Brotações adventícias de abacaxizeiro ornamental sob o efeito de benzilaminopurina, ácido naftalenoacético e períodos de subcultivo Adventitious shoot of ornamental pineapple under benzylaminopurine, naphthalene acetic acid and subculture period effect

    Directory of Open Access Journals (Sweden)

    Maria do Desterro Mendes Santos

    2008-09-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos da adição de benzilaminopurina (BAP, em combinação com o ácido naftalenoacético (ANA, e dos períodos de subcultivo, na formação de brotações in vitro de abacaxizeiro ornamental. O meio básico líquido consistiu de sais minerais MS e vitaminas. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 6x2x4, com seis concentrações de BAP (0, 0,125, 0,25, 0,5, 1 e 2 mg L-1 e duas de ANA (0 e 0,1 mg L-1, e quatro períodos de subcultivo (30, 60, 90 e 120 dias. Os explantes consistiram de brotações individuais de 1 cm de comprimento, oriundas da cultura in vitro. A formação de novas brotações foi observada em meio suplementado com BAP, em todos os períodos de subcultivo. O maior número médio de brotos por explante foi obtido em meio com a concentração de 1,2 mg L-1 de BAP e 120 dias de subcultivo.The objective of this work was to evaluate the effect of benzylaminopurine (BAP additions, in combination with naphthaleneacetic acid (NAA, and of subcultures periods on in vitro shoot formation of ornamental pineapple. The basal liquid medium consisted of MS salts and vitamins. The experiment was a completely randomized design, in a factorial arrangement 6x2x4, with six concentrations of BAP (0, 0.125, 0.25, 0.5, 1, e 2 mg L-1 and two of NAA (0 and 0.1 mg L-1, and four subculturing periods (30, 60, 90 e 120 days. The explants consisted of individualized shoots with 1 cm length from in vitro cultures. Shoot formation was observed in medium supplemented with BAP in all subculturing periods. The greatest average number of shoots per explant was obtained with BAP concentration of 1.2 mg L-1 and 120 days of subcultive.

  19. Sistema para la gestión de nomencladores System for managing nomenclatures

    OpenAIRE

    José Mojena Alpizar; Renán Vázquez Moreno

    2012-01-01

    A partir de la necesidad de gestionar información común y poco variable en el tiempo se crea el Sistema Nomenclador del sistema Balance y Planificación de Insumos Médicos (alas BAP), con el objetivo de centralizar la gestión de los nomencladores. Dicho módulo presentaba deficiencias en su funcionamiento: no existían servicios Web que permitieran acceso a la información de los nomencladores por otros sistemas y presentaba una dependencia total del sistema alas BAP, por lo que se dio la tarea d...

  20. Detection of benzo[a]pyrene diol epoxide-DNA adducts in peripheral blood lymphocytes and antibodies to the adducts in serum from coke oven workers.

    OpenAIRE

    Harris, C. C.; Vahakangas, K.; Newman, M J; Trivers, G E; Shamsuddin, A; Sinopoli, N; Mann, D L; Wright, W. E.

    1985-01-01

    Coke oven workers are exposed to high levels of carcinogenic polycyclic aromatic hydrocarbons, including benzo[a]pyrene (B[a]P), and are at increased risk of lung cancer. Since B[a]P is enzymatically activated to 7 beta,8 alpha-dihydroxy(9 alpha, 10 alpha)epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (B[a]PDE) that forms adducts with DNA, the presence of these adducts was measured in DNA from peripheral blood lymphocytes by synchronous fluorescence spectrophotometry and enzyme radioimmunoassay. App...

  1. In vitro propagation of a forest tree Paulownia tomentosa ( Thunb.) Steud. - A valuable medicinal tree species

    OpenAIRE

    Nada Ben Bahri; Taoufik Bettaieb

    2013-01-01

    The micropropagation of Paulownia tomentosa (Thunb.) Steud. was achieved by culturing nodal explants in MS medium through adding growth regulators: 6-benzylamino purine (BAP), indole-3-butyric acid (IBA) alone or combined in order to initiate shoot bud. Shoot proliferation was induced by the mean of MS medium containing different concentrations of BAP (1or 2 mg L-1) alone or in combination with IBA (0.25 or 0.5 mg L-1). Shoot buds were also placed on MS medium and added with IBA (0.25, 0.5 an...

  2. Plant Regeneration from Callus Culture of Clematis gouriana Roxb. – A Rare Medicinal Plant

    OpenAIRE

    NAIKA, Hanumanaika RAJA; KRISHNA, Venkatarangaiah

    2008-01-01

    An in vitro regeneration protocol through stem callus culture has been standardized for the medicinal climber Clematis gouriana. The explant induced callus on MS-medium supplemented with 0.5 to 1.5 mg l-1 BAP and 0.1 to 0.5 mg l-1 NAA. The optimized callus induction occurred at the concentration of 1.0 mg l-1 BAP and 0.3 mg l-1 NAA. After initiation of callus, it was immediately transferred to MS medium containing 4.0 mg l-1 FAP and 0.5 mg l-1 indole-3-butyric acid (IBA). Upon longer incubati...

  3. Research to establish effects of explant sources and plant growth regulators on camelina (Camelina sativa L. crantz ) tiller and plant induction

    OpenAIRE

    Göre, Merve; KURT, Orhan

    2015-01-01

    This research was conducted in the Biotechnology and Research Application Laboratory Department of Field Crops, Faculty of Agriculture, Ondokuz Mayis University, Samsun-Turkey. In this study Vniimk-17 and Ames-26686 camelina varieties used as a plant material. The root, first internode, 2nd internode, first leaf and 2nd leaf were used as source of explants. Three different media, for plantlets (MS), for callus and tiller induction (ı. MS+0.5 mg/l BAP, ıı. MS+1 g/l BAP, ııı. MS+0.5 g/l NAA, ı...

  4. Effet des différentes balances hormonales sur la microprogation du Cv. Hybride FHIA-01 (Musa sp.)

    OpenAIRE

    Mazinga, KM.; Van Koninckxloo, M.; Godoy Jara, M.; Baboy Longanza, L.

    2013-01-01

    Effects of Different Hormonal Balances on the Microprogation of Cv. Hybrid FHIA-01 (Musa sp.). Different hormonal balances were evaluated to develop a growth medium to effectively multiply the FHIA-01 hybrid in vitro The most striking results of this study are: the almost complete inhibition of the shoot proliferation by BAP; the total root inhibition by the concentration 10 μM BAP+10 μM MemTR and the improvement of the mean shoot proliferation (5,4 shoots/explants) by adding the same concent...

  5. Benzo pyrene-induced DNA adducts and gene expression profiles in target and non-target organs for carcinogenesis in mice

    OpenAIRE

    Zuo, Jie; Brewer, Daniel S; Arlt, Volker M.; Colin S. Cooper; Phillips, David H.

    2014-01-01

    Background Gene expression changes induced by carcinogens may identify differences in molecular function between target and non-target organs. Target organs for benzo[a]pyrene (BaP) carcinogenicity in mice (lung, spleen and forestomach) and three non-target organs (liver, colon and glandular stomach) were investigated for DNA adducts by 32P-postlabelling, for gene expression changes by cDNA microarray and for miRNA expression changes by miRNA microarray after exposure of animals to BaP. Resul...

  6. [Isolation of highly-effective benzo[a]pyrene degrading strain and its degradation capacity].

    Science.gov (United States)

    Liu, Hai-Bin; Wang, Cui-Ping; Zhang, Zhi-Yuan; Xu, Wei; Hao, Zhi-Neng; Sun, Hong-Wen

    2011-09-01

    One new mycete, which could degrade high concentration (up to 100 mg/L) of benzo[a]pyrene (BaP) in liquid, was isolated from contaminated soil of Beijing Coking Plant by gradually increasing the concentration of BaP in mineral salt medium (MSM) in order to get new microorganism species for remediation of BaP contamination. The strain was identified as Lasiodiplodia theobromae, and its biodegradation ability in liquid was further investigated. The results showed that L. theobromae could utilize BaP as sole carbon and energy sources. The experiment was conducted for 10 days, and the biodegradation rate of BaP was 52.5% +/- 1.5%. Compared to Czapek's mineral medium, MSM was more suitable for L. theobromae, and biodegradation rate was 2.8 percent greater than that by using Czapek's mineral media after 10 days' cultivation. Potato-dextrose nutrient medium could accelrate the biodegradation in early stage, and biodegradation rate of BaP increased by 19.2 percent in the second day. However, the accelration was not significant in the latter period, biodegradation rate was only increased by 5.4 percent after 10 days' cultivation. L. theobromae could tolerate a wide pH range, with the optimum pH of 5. Addition of salicylic and sodium succinate enhanced the biodegradation rates by 6.2 percent and 4.2 percent, respectively, after 10 days' cultivation. Besides BaP, L. theobromae could also degrade high concentration (200 mg/L) of phenanthrene and pyrene, and the biodegradation rates were 70.0% +/- 1.0%, 59.2% +/- 3.2%, and 52.5% +/- 1.5% when they were single substrate and were 21.6% +/- 2.1%, 14.5% +/- 5.5%, and 11.9% +/- 2.2% when they existed in mixture, respectively. The biodegradation rate followed an order of phenanthrene > pyrene > BaP. The co-existence of the three substrates led a reduction in biodegradation. This study provides a new microorganism species for remediation of polycyclic aromatic hydrocarbons (PAHs) contamination in the environment. PMID:22165241

  7. Molecular cloning of the wild-type phoM operon in Escherichia coli K-12.

    OpenAIRE

    Wanner, B L; Wilmes, M R; Hunter, E

    1988-01-01

    A metastable bacterial alkaline phosphatase (Bap) phenotype is seen in phoR mutants, which alternately express a Bap-constitutive or -negative phenotype. The alteration is affected by mutations in the phoM region near 0 min. By molecular cloning of the wild-type phoM operon onto a multicopy plasmid and recombining onto the plasmid the pho-510 mutation that abolishes variation, the phoM operon, rather than some nearby gene, was shown to control variation. Complementation tests indicated that t...

  8. Biodegradation of [(sup14)C]Benzo[a]pyrene Added in Crude Oil to Uncontaminated Soil

    OpenAIRE

    Kanaly, R.; Bartha, R.; Fogel, S; Findlay, M

    1997-01-01

    To investigate the possible cometabolic biodegradation of benzo[a]pyrene (BaP), crude oil spiked with [7-(sup14)C]BaP and unlabeled BaP was added to soil with no known pollution history, to give 34 g of oil and 67 mg of BaP/kg of dry soil. The oil-soil mixture was amended with mineral nutrients and incubated in an airtight container with continuous forced aeration. Total CO(inf2) and (sup14)CO(inf2) in the off-gas were trapped and quantified. Soil samples were Soxhlet extracted with dichlorom...

  9. Micropropagação como técnica de rejuvenescimento em mirtilo (Vaccinium ashei Reade cultivar climax Micropropagation as a rejuvenation technique in rabbiteye blueberry (Vaccinium ashei Reade cultivar climax

    Directory of Open Access Journals (Sweden)

    Márcia Wulff Schuch

    2008-06-01

    Full Text Available Objetivou-se com este experimento avaliar o rejuvenescimento do material vegetal através da técnica de micropropagação na produção de mudas de mirtilo, cv. Climax. Os tratamentos aplicados constituíram-se de dois tipos de citocininas (zeatina e 2iP, em quatro concentrações (0; 2,5; 5,0 e 7,5 mg.L-1 e duas fontes de explantes (plantas micropropagadas e plantas obtidas através da germinação de sementes in vitro. O delineamento experimental utilizado foi inteiramente casualizado, em esquema fatorial 2x4x2. Aos 60 dias após a instalação do experimento avaliou-se o número médio de brotos e de gemas por explante, o comprimento médio dos brotos e a taxa de multiplicação. Concluiu-se que plantas de mirtilo, micropropagadas na presença de citocinina e submetidas a sucessivas repicagens, demonstram elevada habilidade de rejuvenescimento in vitro do material adulto, podendo ser comparadas às plantas obtidas de semente, tanto na capacidade de emitir novas brotações, quanto no número de gemas e taxa de multiplicação.This experiment was installed aiming to evaluate the plant rejuvenation capacity of rabbiteye blueberry seedlings cultivar Climax by using micropropagation technique. The treatments consisted of two cytokinin types (zeatin and 2iP, in four concentrations (0; 2.5; 5.0 and 7.5 mg.L-1 and two sources of explants (micropropagated plants and plants from in vitro seeds germination. The experiment was carried out in a 2x4x2 factorial arranged in a randomized complete design. After 60 days from experiment installation it was assessed the shoots average number and shoots average length, buds average number and the multiplication rate. In conclusion, the present results suggest that the micropropagated rabbiteye blueberry plants in the presence of cytokinin and submitted at successive cuttings showed a high in vitro rejuvenation ability of the adult material, which might be compared to the plants germinated in vitro, in the

  10. Action of vegetal regulators, environmental control and storage over parameters of sweet pepper conservation in postharvest Ação de reguladores vegetais, controle ambiental e armazenamento sobre parâmetros de conservação do pimentão em pós-colheita

    Directory of Open Access Journals (Sweden)

    Elisabeth Orica Ono