WorldWideScience

Sample records for cissus quadrangularis cqr-300

  1. The effect of Cissus quadrangularis (CQR-300 and a Cissus formulation (CORE on obesity and obesity-induced oxidative stress

    Directory of Open Access Journals (Sweden)

    Agbor Gabriel A

    2007-02-01

    Full Text Available Abstract Aim Obesity is generally linked to complications in lipid metabolism and oxidative stress. The aim of this study was to compare the effect of a proprietary extract of Cissus quadrangularis (CQR-300 to that of a proprietary formulation containing CQR-300 (CORE on weight, blood lipids, and oxidative stress in overweight and obese people. Methods The first part of the study investigated the in vitro antioxidant properties of CQR-300 and CORE using 3 different methods, while the second part of the study was a double-blind placebo controlled design, involving initially 168 overweight and obese persons (38.7% males; 61.3% females; ages 19–54, of whom 153 completed the study. All participants received two daily doses of CQR-300, CORE, or placebo and were encouraged to maintain their normal levels of physical activity. Anthropometric measurements and blood sampling were done at the beginning and end of the study period. Results CQR-300 as well as CORE exhibited antioxidant properties in vitro. They also acted as in vivo antioxidants, bringing about significant (p Conclusion CQR-300 (300 mg daily and CORE (1028 mg daily brought about significant reductions in weight and blood glucose levels, while decreasing serum lipids thus improving cardiovascular risk factors. The increase in plasma 5-HT and creatinine for both groups hypothesizes a mechanism of controlling appetite and promoting the increase of lean muscle mass by Cissus quadrangularis, thereby supporting the clinical data for weight loss and improving cardiovascular health.

  2. MINERAL AND BIOCHEMICAL ANALYSIS OF VARIOUS PARTS OF CISSUS QUADRANGULARIS LINN

    OpenAIRE

    Udayakumar, R.; Sundaran, M.; Krishna, Raghuram

    2004-01-01

    Ash, minerals and biochemical contents were determined in various parts of root, stem and leaf of Cissus quadrangularis. The maximum ash content was observed in the root. The maximum concentration of carbohydrate and protein in the root and phosphorus, iron, calcium and lipids in the stem were observed.

  3. Ketosteroid Standardized Cissus quadrangularis L. Extract and its Anabolic Activity: Time to Look Beyond Ketosteroid?

    Science.gov (United States)

    Jadhav, Atul N; Rafiq, Mohammed; Devanathan, Rajendran; Azeemuddin, Mohammed; Anturlikar, Suryakanth D; Ahmed, Akhil; Sundaram, Ramchandran; Babu, U V; Paramesh, Rangesh

    2016-05-01

    Cissus quadrangularis (CQ) L. reported to contain 3-ketosteroids and have bone health benefits. This study aimed at establishing the relationship between the ketosteroid content and anabolic as well as bone health-promoting activities of various Cissus extracts in well-established orchidectomized (ORX) rat model. Supercritical carbon dioxide, ethyl acetate, and aqueous extracts (AE) of CQ L. were prepared and standardized for ketosteroid content by two methods used in commerce. Moreover, ketosteroid standardized extracts of this plant were evaluated for anabolic activity in rats in well-established ORX rat model. The increase in the absolute weight was appreciable in the CQ-AE treated group. Similarly, with respect to bone parameters, a similar trend was seen. The mean bone density, strength, and calcium content were found to be highest in the group treated with CQ-AE compared to groups treated with other extracts. This study reveals for the first time that 3-ketosteroids are not linked to the beneficial activities on bone and highlights the need for extensive characterization of biological active principles from CQ L. In light of the above estimation studies, we believe that current standardization of Cissus extraction "3-ketosteroids" is incorrect. We also did not find any report suggesting the presence of androgenic steroids in this plant and hence the characterization based on "3-ketosteroids" is scientifically incorrect. This study highlights the insufficient understanding of biological active principles from CQ L. and underlines the need for extensive bioactivity guided studies. Cissus quadrangularis (CQ) L. reported to contain 3.ketosteroids and have bone health benefitsWe did not find correlation between ketosteroid content obtained by conventional methods and its biological effectStudies indicate that claims of ketosteroid content need not necessarily correlate to biological effects and hence warrants extensive phytochemical characterization of biological

  4. The use of a Cissus quadrangularis formulation in the management of weight loss and metabolic syndrome

    Directory of Open Access Journals (Sweden)

    Agbor Gabriel

    2006-09-01

    Full Text Available Abstract Aim Once considered a problem of developed countries, obesity and obesity-related complications (such as metabolic syndrome are rapidly spreading around the globe. The purpose of the present study was to investigate the use of a Cissus quadrangularis formulation in the management of metabolic syndrome, particularly weight loss and central obesity. Methods The study was a randomized, double-blind, placebo-controlled design involving 123 overweight and obese persons (47.2% male; 52.8% female; ages 19–50. The 92 obese (BMI >30 participants were randomized into three groups; placebo, formulation/no diet, and formulation/diet (2100–2200 calories/day. The 31 overweight participants (BMI = 25–29 formed a fourth (no diet treatment group. All participants received two daily doses of the formulation or placebo and remained on a normal or calorie-controlled diet for 8 weeks. Results At the end of the trial period, statistically significant net reductions in weight and central obesity, as well as in fasting blood glucose, total cholesterol, LDL-cholesterol, triglycerides, and C-reactive protein were observed in participants who received the formulation, regardless of diet. Conclusion Cissus quadrangularis formulation appears to be useful in the management of weight loss and metabolic syndrome.

  5. Effect of Cissus quadrangularis on gastric mucosal defensive factors in experimentally induced gastric ulcer-a comparative study with sucralfate.

    Science.gov (United States)

    Jainu, Mallika; Devi, C S Shyamala

    2004-01-01

    Cissus quadrangularis is an indigenous plant commonly mentioned in Ayurveda for treatment of gastric ulcers. The ulcer-protective effect of a methanolic extract of C. quadrangularis (CQE) was comparable to that of the reference drug sucralfate. Further, gastric juice and mucosal studies showed that CQE at a dose of 500 mg/kg given for 10 days significantly increased the mucosal defensive factors like mucin secretion, mucosal cell proliferation, glycoproteins, and life span of cells. The present investigation suggests that CQE not only strengthens mucosal resistance against ulcerogens but also promotes healing by inducing cellular proliferation. Thus, CQE has potential usefulness for treatment of peptic ulcer disease.

  6. Poly(L-lactic acid) nanofibers containing Cissus quadrangularis induced osteogenic differentiation in vitro.

    Science.gov (United States)

    Parvathi, K; Krishnan, Amit G; Anitha, A; Jayakumar, R; Nair, Manitha B

    2018-04-15

    Cissus quadrangularis (CQ) is known as "bone setter" in Ayurvedic Medicine because of its ability to promote fracture healing. Polymers incorporated with CQ at lower concentration have shown to enhance osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro. However, for the healing of clinically relevant critical sized bone defects, large amount of CQ would be required. Based on this perception, a herbal fibrous sheet containing high weight percentage of CQ [20,40 and 60wt/wt% in poly (L-lactic acid) (PLLA)] was fabricated through electrospinning. The solution concentration, flow rate, voltage and tip-target distance was optimized to obtain nanofibers. The hydrophobicity of PLLA fibers was reduced through CQ incorporation. There was considerable increase in the adhesion, proliferation and osteogenic differentiation of MSCs on herbal fibers than normal fibers, mainly on P-Q20 and P-CQ40. MSCs were differentiated into osteoblasts without providing any osteogenic supplements in the medium, indicating its osteoinductive capability. The herbal sheet also could promote mineralization when immersed in simulated body fluid for 14days. These studies specify that PLLA nanofibers loaded with 20 and 40wt% of CQ could serve as a potential candidate for bone tissue engineering applications. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. A novel high-performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from Cissus quadrangularis

    Directory of Open Access Journals (Sweden)

    Vandana Jain

    2016-08-01

    Full Text Available Context: A high-performance thin layer chromatography (HPTLC is an analytical technique, which can be used for the determination of constituents or marker components in various parts of the plants. Earlier studies have estimated phytoconstituents from the stem and other aerial plant parts of Cissus quadrangularis Linn. Estimation of hydrocarbons can also be successfully done using HPTLC technique using suitable derivatization. Aims: To develop and validate a simple and rapid method for the estimation of long chain aliphatic hydrocarbons from the leaves of C. quadrangularis using HPTLC technique. Methods: Precoated silica gel 60 F254 plates were used as stationary phase. The mobile phase used was hexane (100 %. The detection of spots was carried out using berberine sulphate as detecting reagent. Results: The method was validated in terms of linearity, sensitivity, accuracy, and precision. Linearity range was found to be 2-10 µg/mL, limit of detection 0.127 µg/mL, and limit of quantification 0.384 µg/mL. Conclusions: A novel, simple, accurate, precise and sensitive HPTLC method has been developed and validated for the estimation of long chain aliphatic hydrocarbons obtained from the leaves of C. quadrangularis Linn.

  8. Cissus quadrangularis mediated ecofriendly synthesis of copper oxide nanoparticles and its antifungal studies against Aspergillus niger, Aspergillus flavus.

    Science.gov (United States)

    Devipriya, Duraipandi; Roopan, Selvaraj Mohana

    2017-11-01

    Recently, non-toxic source mediated synthesis of metal and a metal oxide nanoparticle attains more attention due to key applicational responsibilities. This present report stated that the eco-friendly synthesis of copper oxide nanoparticles (CuO NPs) using Cissus quadrangularis (C. quadrangularis) plant extract. Further the eco-friendly synthesized CuO NPs were characterized using a number of analytical techniques. The observed results stated that the synthesized CuO NPs were spherical in shape with 30±2nm. Then the eco-friendly synthesized CuO NPs were subjected for anti-fungal against two strains namely Aspergillus niger (A. niger) resulted in 83% at 500ppm, 86% of inhibition at 1000ppm and Aspergillus flavus (A. flavus) resulted in 81% at 500ppm, 85% of inhibition at 1000ppm respectively. Despite the fact that compared to standard Carbendazim, eco-friendly synthesized CuO NPs exhibits better results were discussed in this manuscript. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Cissus quadrangularis inhibits IL-1β induced inflammatory responses on chondrocytes and alleviates bone deterioration in osteotomized rats via p38 MAPK signaling

    Directory of Open Access Journals (Sweden)

    Kanwar JR

    2015-06-01

    Full Text Available Jagat R Kanwar,1 Rasika M Samarasinghe,1 Kuldeep Kumar,2 Ramesh Arya,2 Sanjeev Sharma,2 Shu-Feng Zhou,3 Sreenivasan Sasidharan,4 Rupinder K Kanwar11Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR, School of Medicine (SoM, Molecular and Medical Research (MMR Strategic Research Centre, Faculty of Health, Geelong Technology Precinct (GTP, Deakin University, Waurn Ponds, VIC, Australia; 2Ayurvedic College, Paprola, Kangra, Himachal Pradesh, India; 3Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, Tampa, FL, USA; 4Institute for Research in Molecular Medicine (INFORMM, Universiti Sains Malaysia, Penang, MalaysiaIntroduction: Inflammatory mediators are key players in the pathogenesis of osteoarthritis (OA and bone destruction. Conventional drugs suppress symptomatic activity and have no therapeutic influence on disease. Cissus quadrangularis and Withania somnifera are widely used for the treatment of bone fractures and wounds; however, the cellular and molecular mechanisms regulated by these herbals are still unclear.Methods: We established an in vitro OA culture model by exposing human chondrocytes to proinflammatory cytokine and interleukin (IL-1β for 36 hours prior to treatment with the herbals: C. quadrangularis, W. somnifera, and the combination of the two herbals. Cell viability, toxicity, and gene expression of OA modifying agents were examined. In addition, expression of survivin, which is crucial for cell growth, was analyzed. In vivo work on osteotomized rats studied the bone and cartilage regenerative effects of C. quadrangularis, W. somnifera, and the combination therapy.Results: Exposure of chondrocytes to IL-1β induced significant toxicity and cell death. However, herbal treatment alleviated IL-1β induced cell toxicity and upregulated cell growth and proliferation. C. quadrangularis inhibited gene expression of cytokines and matrix metalloproteinases, known to

  10. In Vitro Anthelmintic Activity of Crude Extracts of Aerial Parts of Cissus quadrangularis L. and Leaves of Schinus molle L. against Haemonchus contortus

    Directory of Open Access Journals (Sweden)

    Selamawit Zenebe

    2017-01-01

    Full Text Available Background. Haemonchus contortus, the causative agent of Haemonchosis, is the most economically important parasite in small ruminant production. Control with chemotherapy has not been successful due to rapid emergence of drug-resistant strains. There is a continuous search for alternative leads particularly from plants. The study aimed to evaluate the anthelmintic activity of crude methanolic extracts of leaves of Schinus molle and aerial parts of Cissus quadrangularis against H. contortus. Methods. Adult motility test and egg hatching inhibition assay were employed to investigate the in vitro adulticidal and egg hatching inhibitory effects of the extracts. Results. Higher concentrations of the extracts (10 and 5 mg/ml had a significantly superior adulticidal activity (p<0.05 compared to the negative control and lower concentration levels, which was comparable to albendazole. Similarly, the relative egg hatch inhibition efficacy of S. molle and C. quadrangularis extracts indicated a maximum of 96% and 88% egg hatch inhibition, respectively, within the 48 hrs of exposure at 1 mg/ml. Conclusion. The current study evidenced that the crude methanolic extracts of the plants have promising adulticidal and egg hatching inhibitory effects against H. contortus.

  11. In Vitro Anthelmintic Activity of Crude Extracts of Aerial Parts of Cissus quadrangularis L. and Leaves of Schinus molle L. against Haemonchus contortus.

    Science.gov (United States)

    Zenebe, Selamawit; Feyera, Teka; Assefa, Solomon

    2017-01-01

    Haemonchus contortus, the causative agent of Haemonchosis, is the most economically important parasite in small ruminant production. Control with chemotherapy has not been successful due to rapid emergence of drug-resistant strains. There is a continuous search for alternative leads particularly from plants. The study aimed to evaluate the anthelmintic activity of crude methanolic extracts of leaves of Schinus molle and aerial parts of Cissus quadrangularis against H. contortus. Methods . Adult motility test and egg hatching inhibition assay were employed to investigate the in vitro adulticidal and egg hatching inhibitory effects of the extracts. Higher concentrations of the extracts (10 and 5 mg/ml) had a significantly superior adulticidal activity ( p molle and C. quadrangularis extracts indicated a maximum of 96% and 88% egg hatch inhibition, respectively, within the 48 hrs of exposure at 1 mg/ml. The current study evidenced that the crude methanolic extracts of the plants have promising adulticidal and egg hatching inhibitory effects against H. contortus .

  12. Effects of ethanol extract of Cissus quadrangularis on induced ...

    African Journals Online (AJOL)

    aghomotsegin

    2013-10-23

    Oct 23, 2013 ... The use of plants and its extracts have aided mankind in the treatment of different ... about three-quarters of the world population rely on plants for the ... Chemical balance (Gallenkamp England), plastic container. (Gallenkamp ...

  13. ANTHRAQUINONES OF CISSUS POPULNEA GUILL & PERR ...

    African Journals Online (AJOL)

    Cissus populnea has been used locally to treat many ailments such as venereal, stomach and skin infections; and also used as laxative or purgative. Economically it has been used as binder in food and in lining dye pits. This work aims at determining the type of anthraquinones from the stem bark of C. populnea which ...

  14. Effects of Cissus Populnea (Ogbolo) On Mainstream Smoked ...

    African Journals Online (AJOL)

    Effects of Cissus Populnea (Ogbolo) On Mainstream Smoked Weaned Rabbit. H A Smith, O Ogunfeibo. Abstract. The effect of Cissus populnae, a medicinal plant commonly used by Nigerian Traditional Medicine practitioners for the treatment of fertility abnormalities especially in males has been investigated in smoking ...

  15. Effects Of Cissus Populnea (Ogbolo) On Mainstream Smoked ...

    African Journals Online (AJOL)

    Effects Of Cissus Populnea (Ogbolo) On Mainstream Smoked Weaned Rabbit. H A Smith, O Ogunfeibo. Abstract. The effect of Cissus populnea, a medicinal plant commonly used by the Nigerian Traditional medicine practitioners for the treatment of tertiary abnormalities especially in male has been investigated in smoking ...

  16. Flavonoid Glycosides from the Leaves of Cissus Ibuensis Hook (Vitaceae)

    OpenAIRE

    Ahmadu, AA; Onanuga, A; Aquino, R

    2010-01-01

    The bioactive N-butanol fraction of the ethanol extract of the leaves of Cissus ibuensis was fractionated over silica Gel column to give Quercetin 3-O-rutinoside (I) and mixtures of Flavonoids (A2). A2 was fractionated using reverse phase HPLC to give Kaempferol 3-O-α-rhamnopyranosyl (1→6)-β;-D-galactopyranoside (II), Kaempferol 3-O-rutinoside(III)and Kaempferol3-O-α-rhamnopyranosyl (1→6)-α-rhamnopyranosyl(1→2)-β;-D-galactopyranoside (IV). The structures were elucidated by NMR spectroscopy an...

  17. Flavonoid glycosides from the leaves of Cissus ibuensis hook (vitaceae).

    Science.gov (United States)

    Ahmadu, A A; Onanuga, A; Aquino, R

    2010-04-03

    The bioactive N-butanol fraction of the ethanol extract of the leaves of Cissus ibuensis was fractionated over silica Gel column to give Quercetin 3-O-rutinoside (I) and mixtures of Flavonoids (A2). A2 was fractionated using reverse phase HPLC to give Kaempferol 3-O-α-rhamnopyranosyl (1→6)-β-D-galactopyranoside (II), Kaempferol 3-O-rutinoside(III) and Kaempferol3-O-α-rhamnopyranosyl (1→6)-α-rhamnopyranosyl(1→2)-β-D-galactopyranoside (IV). The structures were elucidated by NMR spectroscopy and compared with literature.

  18. Estudio de la propagación sexual del arboloco Montanoa quadrangularis Schultz Bipontianus Asteraceae

    Directory of Open Access Journals (Sweden)

    William Escobar-Torres

    2010-04-01

    Full Text Available Sexual propagation of arboloco Montanoa quadrangularis Schultz Bipontianus (Asteraceae. Objective. To develop sexual propagationmethodologies for Montanoa quadrangularis Schultz Bipontianus (arboloco. Materials and methods. Seeds were harvested from treesgrown at the Chilaca environmental station (Geoambiente Ltda. located in Pacho, Cundinamarca, Colombia. The study was carried out intwo phases: firstly, substrate effect on germination responses was evaluated in seedbed conditions. Substrates evaluated were: local soil,local soil mixed with worm humus, and peat. Additionally, two spatial arrangements were assessed: seeds sown at random and at 0.5 cmin depth. Secondly, in nursery conditions the effect of the substrates local soil, local soil mixed with worm humus and peat, as well as theeffect of developmental stages of the plants (with 2, 4, and 6 leaves on their further growth and development were evaluated. Results. Inseedbed conditions, the germination capacity was highly influenced by peat substrate (83.67% whereas in local soil the germinationcapacity was 40.83%. In nursery conditions, 6-leaf plants grown in local soil mixed with worm humus showed the best responses onparameters such as plant total length (9.51 cm, leaf area (36.69 cm2, and total dry weight (0.10 g. Conclusions. The type of substrateused in seedbed conditions influenced the germination capacity of M. quadrangularis seeds. The substrate and the developmental stage ofthe plant had a significant influence on the growth and development of arboloco plants.

  19. Design of cissus-alginate microbeads revealing mucoprotection properties in anti-inflammatory therapy.

    Science.gov (United States)

    Okunlola, Adenike; Odeku, Oluwatoyin A; Lamprecht, Alf; Oyagbemi, Ademola A; Oridupa, Olayinka A; Aina, Oluwasanmi O

    2015-08-01

    Cissus gum has been employed as polymer with sodium alginate in the formulation of diclofenac microbeads and the in vivo mucoprotective properties of the polymer in anti-inflammatory therapy assessed in rats with carrageenan-induced paw edema in comparison to diclofenac powder and commercial diclofenac tablet. A full 2(3) factorial experimental design has been used to investigate the influence of concentration of cissus gum (X1); concentration of calcium acetate (X2) and stirring speed (X3) on properties of the microbeads. Optimized small discrete microbeads with size of 1.22±0.10 mm, entrapment efficiency of 84.6% and t80 of 15.2±3.5 h were obtained at ratio of cissus gum:alginate (1:1), low concentration of calcium acetate (5% w/v) and high stirring speed (400 rpm). In vivo studies showed that the ranking of percent inhibition of inflammation after 3h was diclofenac powder>commercial tablet=cissus>alginate. Histological damage score and parietal cell density were lower while crypt depth and mucosal width were significantly higher (pdiclofenac microbeads than those administered with diclofenac powder and commercial tablet, suggesting the mucoprotective property of the gum. Thus, cissus gum could be suitable as polymer in the formulation of non-steroidal anti-inflammatory drugs ensuring sustained release while reducing gastric side effects. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. Evaluation of the Antidiabetic and Antibacterial Activity of Cissus sicyoides

    Directory of Open Access Journals (Sweden)

    Flavio Luis Beltrame

    2002-03-01

    Full Text Available In this work we investigated the antidiabetic and antibacterial effect of Cissus sicyoides (CS from Brazil. Diabetic rats that received water (A group or extracts from the aerial parts of the plant (Cs group during four weeks were employed. After this period, serum levels of glucose, cholesterol and triglycerides were measured. Glycemia was not affected by treatment with CS. However, there was an increased cholesterol and triglyceride level in Cs group. In addition, bioassay-guided fractionation of methanolic extract from aerial parts of CS was performed for isolation of antibacterial compounds.beta-Sitosterol and sitosterol-beta-D-glucopyranoside isolated showed antibacterial activity against Bacillus subtilis with minimal inhibitory concentrations (MICs of 50 mug/ml and 100 mug/ml, respectively. In spite of popular belief, CS did not show antidiabetic activity. However, two compounds isolated from aerial parts of the plant (beta-sitosterol and sitosterol-beta-D-glucopyranoside showed antibacterial activity.No presente trabalho foram investigados os efeitos antibacteriano e antidiabético da planta Cissus sicyoides (CS coletada no Brasil. Ratos diabéticos receberam água (grupo A ou extratos da parte aérea da planta (grupo CS durante 4 semanas. Após este período, os níveis séricos de glicose, colesterol e triglicerídeos dos ratos foram determinados. A glicemia não foi afetada pelo tratamento com CS. Entretanto, houve aumento nos níveis de colesterol e triglicerídeos nos ratos do grupo CS. Em adição, fracionamento bio-monitorado foi realizado para o isolamento de compostos com atividade antibacteriana. beta-Sitosterol e sitosterol-beta-D-glucopiranosídeo isolados mostram atividade antibacteriana contra Bacillus subtilis com concentrações mínimas inibitórias (MICs de 50 mig/ml e 100 mig/ml, respectivamente. Apesar da crença popular, CS não mostrou atividade antidiabética. Entretanto, dois compostos isolados da parte aérea da

  1. Long term effects of aqueous stem bark extract of Cissus populnea ...

    African Journals Online (AJOL)

    AJB SERVER

    2007-02-05

    Feb 5, 2007 ... Full Length Research Paper. Long term effects of aqueous stem bark extract of. Cissus populnea (Guill. and Per.) on some biochemical ... study period revealed that continuous exposure of the plant extract had no damaging effects on the organs of xenobiotic metabolism (liver and kidney). results of levels ...

  2. Involvement of GABAergic pathway in the sedative activity of apigenin, the main flavonoid from Passiflora quadrangularis pericarp

    Directory of Open Access Journals (Sweden)

    Andressa C. Gazola

    Full Text Available Abstract In the current study we showed that oral administration of an aqueous extract of Passiflora quadrangularis L., Passifloraceae, pericarp results in a significant prolongation of the sleep duration in mice evaluated in the ethyl ether-induced hypnosis test which indicates sedative effects. Apigenin, the main flavonoid of the extract, induced a similar sedative response when applied alone, at a dose equivalent to that found in the extract, suggesting that apigenin is mediating the sedative effects of P. quadrangularis extract. In addition, the sedative effect of apigenin was blocked by pretreatment with the benzodiazepine antagonist flumazenil (1 mg/kg, suggesting an interaction of apigenin with gamma-aminobutyric acid type A (GABAA receptors. However, apigenin at concentrations 0.1–50 µM failed to enhance GABA-induced currents through GABAA receptors (α1β2γ2S expressed in Xenopus oocytes. Nevertheless, based on our results, we suggest that the in vivo sedative effect of the P. quadrangularis extract and its main flavonoid apigenin maybe be due to an enhancement of the GABAergic system.

  3. Contribución al estudio de Cissus sicyoides L. (bejuco-ubí

    Directory of Open Access Journals (Sweden)

    Ramón Scull Lizama

    2000-08-01

    Full Text Available Se presenta la descripción micromorfológica de Cissus sicyoides L. (bejuco-ubí y se resume la composición fitoquímica preliminar, donde se detecta la existencia de aminoácidos, compuestos grasos y flavonoides en toda la parte aérea de la planta, los cuales no habían sido informados con anterioridad en la bibliografía. Se demuestra que el tipo de secado no altera la composición de la droga y se marcan pautas sobre la estabilidad de ésta. Se comprueba que la planta no posee efectos tóxicos ni antivirales contra el virus de la influenza A en cepas victoria H3N2 y WSN (H1N1, para las condiciones en que se desarrolló el estudio.The micromorphological description of Cissus sicoydes L. (ipecacuanha is presented and the preliminary phytochemical composition is summarized. Aminoacids, fatty compounds and flavonoids, which have not been previously reported in bibliography, are detected in the whole aerial part of the plant. It is proved that the type of drying does not alter the compositon of the drug and guidelines are set on its stability. It is also demonstrated that the plant does not have toxic or antiviral effects against the virus of influenza A in victoria H3N2 and WSN (H1N1 strains for the conditons under which the study was developed.

  4. Micropropagación de Cissus tiliacea, planta del sur del estado de México

    OpenAIRE

    José Humberto Jiménez-Martínez

    2011-01-01

    Se micropropagó Cissus tiliace, recurso fitogenético con potencial agronómico y farmacológico, en los medios de cultivo Murashige-Skoog (MS) y Lloyd y McCown (WPM). En ambos medios se generaron resultados similares para número de brotes, nudos, hojas y raíces adventicias, sólo existió diferencia significativa (p 0,05) en la formación de callo. Para la multiplicación in vitro se utilizó WPM adicionado con 0; 0,5; 1,0; 1,5 ó 2,0 mg L-1de benciladenina (BA) y se emplearon tres tipos de segment...

  5. Analgesic and Anti-Inflammatory Activities of Methanol Extract of Cissus repens in Mice

    Directory of Open Access Journals (Sweden)

    Ching-Wen Chang

    2012-01-01

    Full Text Available The aim of this study was to investigate possible analgesic and anti-inflammatory mechanisms of the CRMeOH. Analgesic effect was evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The anti-inflammatory effect was evaluated by λ-carrageenan-induced mouse paw edema and histopathologic analyses. The results showed that CRMeOH (500 mg/kg decreased writhing response in the acetic acid assay and licking time in the formalin test. CRMeOH (100 and 500 mg/kg significantly decreased edema paw volume at 4th to 5th hours after λ-carrageenan had been injected. Histopathologically, CRMeOH abated the level of tissue destruction and swelling of the edema paws. These results were indicated that anti-inflammatory mechanism of CRMeOH may be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx, and GRd in the liver. Additionally, CRMeOH also decreased IL-1β, IL-6, NFκB, TNF-α, COX-2, and iNOS levels. The contents of two active ingredients, ursolic acid and lupeol, were quantitatively determined. This paper demonstrated possible mechanisms for the analgesic and anti-inflammatory effects of CRMeOH and provided evidence for the classical treatment of Cissus repens in inflammatory diseases.

  6. Estudo fitoquímico e avaliação do potencial antidiabético do Cissus sicyoides L. (Vitaceae Phytochemical study and evaluation of the antidiabetic potential of Cissus sicyoides L. (Vitaceae

    Directory of Open Access Journals (Sweden)

    Flávio Luís Beltrame

    2001-12-01

    Full Text Available The effect of hydroalcoholic extracts (HE obtained from leaves of Cissus sicyoides (CS on glucose tolerance (GT was investigated in rats treated with dexametasone (DEX. Our results showed that HE intensified the decreased GT promoted by (DEX. Additionally, the flavonoids kaempferol 3-O-rhamnoside and quercetin 3-O-rhamnoside, obtained from aerial parts of CS, were used to study the incorporation of glucose to glycogen in soleo muscle. The results showed that both flavonoids did not show effect on glycogen synthesis. Thus, our data, in contrast to popular believe, did not reveal antidiabetic activity to SC.

  7. Cytotoxic, antitumor and leukocyte migration activities of resveratrol and sitosterol present in the hidroalcoholic extract of Cissus sicyoides L., Vitaceae, leaves

    Directory of Open Access Journals (Sweden)

    Flávia R. S. Lucena

    2010-05-01

    Full Text Available Cissus sicyoides L. pertains to the Vitaceae family. It is popularly known as "insulina, cipo-pucá, bejuco caro, puci, anil trepador". A vasoconstrictor effect and an antibacterial activity have also been allocated to it. In Brazil, C. sicyoides was evaluated for its anticonvulsant and anti-diabetc properties. Phytochemistry studies identified and isolated sitosterol and resveratrol compounds from its aerial parts which are pointed out as having antitumor activities. The goal of this study was to investigate the cytotoxic and antitumor activities of Cissus sicyoides hydroalcoholic extract as well as its ability to repair leukocytes cells to injured tissue. Cissus sicyoides did not demonstrate cytotoxic activity but showed an inhibition of tumor growth in face of the tumors tested. The extract had a strong chemotactic effect on the twenty four hours period after treatment. The hidroalchoolic extract of Cissus sicyoides presented antitumor activity which was prompted by T lymphocytes recruitment to the local lesion and suggests a new pathway to antitumor activity by activation of lymphoid lineage.

  8. Hypoglycemic and anti-lipemic effects of the aqueous extract from Cissus sicyoides

    Science.gov (United States)

    Viana, Glauce SB; Medeiros, Ana Carolina C; Lacerda, Ana Michelle R; Leal, L Kalyne AM; Vale, Tiago G; Matos, F José de Abreu

    2004-01-01

    Background Cissus sicyoides (Vitaceae) is a medicinal plant popularly known in Brazil as "cipó-pucá, anil-trepador, cortina, and insulina". The plant is used in several diseases, including rheumatism, epilepsy, stroke and also in the treatment of diabetes. In the present work, we studied the hypoglycemic and anti-lipemic effects of the aqueous extract prepared from fresh leaves of the plant (AECS), in the model of alloxan-induced diabetes in rats. In addition, hepatic enzyme levels were also determined. Results Results showed that the daily treatment of diabetic rats with AECS for 7 days (100 and 200 mg/kg, p.o.) significantly decreased blood glucose levels in 25 and 22% respectively, as compared to the same groups before AECS treatment. No significant changes were seen in control diabetic rats before (48 h after alloxan administration) and after distilled water treatment. While no changes were seen in total cholesterol levels, a significant decrease was observed in plasma triglyceride levels, in the alloxan-induced diabetic rats after AECS treatment with both doses, as compared to the same groups before treatment. Significant decreases in blood glucose (25%) and triglyceride levels (48%) were also observed in the alloxan-induced diabetic rats after 4 days treatment with AECS (200 mg/kg, p.o.). Aspartate (AST) and alanine (ALT) aminotransferases levels, in diabetic controls and AECS-treated rats, were in the range of reference values presented by normal rats. Conclusions The results justify the popular use of C. sicyoides, pointing out to the potential benefit of the plant aqueous extract (AECS) in alternative medicine, in the treatment of type 2 diabetes mellitus. PMID:15182373

  9. Calogênese em Cissus sicyoides L. a partir de segmentos foliares visando à produção de metabólitos in vitro Calluses from Cissus sicyoides L. leaves

    Directory of Open Access Journals (Sweden)

    F.R. Rodrigues

    2010-09-01

    Full Text Available Os metabólitos secundários são essencialmente produzidos e extraídos a partir de plantas cultivadas no campo sobre a influência de variações sazonais. A utilização de técnicas biotecnológicas apresenta-se como um recurso alternativo para a produção de fármacos. Dentre essas técnicas, destaca-se a cultura de tecidos através da calogênese, uma vez que o crescimento de calos é desejável para induzir variação somaclonal e estudos fisiológicos, principalmente quando se deseja relacionar a presença de metabólitos secundários com o crescimento celular. Assim, o objetivo deste trabalho foi estabelecer um protocolo de calogênese de Cissus sicyoides L., a partir de segmentos foliares visando à produção de metabólitos in vitro. Para o estabelecimento in vitro, foram utilizados como explantes, segmentos foliares retirados de planta adulta cultivada em campo. Após desinfestação, o material foi inoculado em meio MT + 1,0 mg L-1 ANA e mantido em câmara de crescimento tipo BOD, com temperatura e luminosidade controladas. Após 30 dias foram avaliados a porcentagem de explantes sobreviventes e de contaminação. Para o cultivo utilizou-se o meio MT + 1,0 mg L-1 ANA, variando-se as concentrações de BAP em 2,0; 4,0; 6,0 e 12,0 mg L-1. No cultivo avaliou-se o número de calos compactos e friáveis. Para o primeiro e segundo subcultivo o material foi introduzido em meio MT + 1,0 mg L-1 ANA variando-se as mesmas concentrações de BAP, sendo avaliados o número de calos friáveis formados e o tamanho da massa de calos. Foi obtido ainda o número de repetições formadas no decorrer dos subcultivos, peso da matéria fresca (g e seca (g. Em seguida, foram realizados os testes fitoquímicos para identificação de alguns constituintes. Concluiu-se que o tempo e a concentração de hipoclorito de sódio utilizado, mostraram-se pouco eficientes para a desinfestação. Para a calogênese de Cissus sicyoides L. a partir de segmento foliar

  10. Browse Title Index

    African Journals Online (AJOL)

    Items 151 - 200 of 316 ... Vol 11 (2007), Evaluation Of Antimitotic And Anticancer Activity Of ... CYTOPROTECTIVE EFFECTS OF CISSUS QUADRANGULARIS L. VARIAN II IN RATS. ... Vol 11 (2007), Hepatoprotective Action Of Cordia dichotoma ...

  11. Fenologia do florescimento e características do perfume das flores de Passiflora quadrangularis l. (maracujá-melão

    Directory of Open Access Journals (Sweden)

    Daniel Antonio Villamil Montero

    2013-08-01

    Full Text Available Passiflora quadrangularis L. é uma espécie originária do neotrópico, conhecida no Brasil como maracujá-melão devido ao tamanho e formato dos seus frutos. Tem sido amplamente disseminada nas regiões tropicais do mundo onde é produzida em pequena escala para comercialização dos frutos, o que ocorre bem próximo às regiões de cultivo, em função da dificuldade de conservação pós-colheita. As plantas são trepadeiras bastante vigorosas, com flores abundantes, grandes e perfumadas, o que amplia o seu valor e a torna particularmente interessante para caramanchões e cercas-vivas. O uso efetivo de passifloras no mercado de plantas ornamentais depende de estudos da fenologia floral. Neste trabalho foram estudadas algumas características da fenologia floral e o perfume das flores de P. quadrangularis, sob cultivo protegido. São apresentados os dados relativos ao período de floração, picos de florescimento e intensidade relativa das flores, bem como algumas características do perfume floral. Conclui-se que a espécie representa uma nova alternativa de cultivo para fins ornamentais, por apresentar múltiplas flores em antese simultânea, abundantes, grandes, fragrantes e coloridas, com longo período de floração no ano, além de frutos comestíveis, folhas exuberantes e medicinais que oferecem amplo sombreamento para áreas externas de lazer e/ou outras plantas cultivadas.

  12. A INFLUÊNCIA DO Cissus chiclete na MECÂNICAS E LIBERTAÇÃO DE PROPRIEDADES PARACETAMOL COMPRIMIDOS - uma análise fatorial The influence of cissus gum on the mechanical and release properties of paracetamol tablets – a factorial analysis

    Directory of Open Access Journals (Sweden)

    ADETAYO O ADELEYE

    2010-06-01

    Full Text Available Os efeitos quantitativos de uma goma nova como a pasta nas propriedades mecânicas e da liberação de formulações da tabuleta do paracetamol foram avaliados usando um projeto 23 experimental factorial cheio. Goma de Cissus obtida do populnea Guill de Cissus. & Perr. (Vitaceae foi comparado com o gelatin oficial. Os efeitos do indivíduo e da interação do tipo de pasta, da concentração de fração da pasta e de embalagem na friabilidade, da força elástica, do índice da fratura frágil, do tempo da desintegração e da liberação da droga das tabuletas eram determinados. Mudar a pasta do gelatin à goma de Cissus conduziu a um aumento na friabilidade e a uma diminuição na força elástica, no BFI e nos parâmetros da liberação da droga. Concentração crescente da pasta de 2.0w/w a 4.0%w/w, e densidade relativa crescente de 0.80 a 0.90, conduzido para aumentar na tendência da laminação e na taxa de liberação das formulações. As tabuletas que contêm o gelatin tiveram uma força elástica mais elevada com mais baixa friabilidade, e uns tempos mais elevados da desintegração e da dissolução com mais tendência laminar quando comparadas com a goma de Cissus. Daqui, o cuidado deve ser recolhido escolhendo uma pasta apropriada para formulações da tabuleta no que diz respeito às características mecânicas e da liberação. O estudo sugere que a goma de Cissus seja preferida ao gelatin nas formulações da tabuleta que exibem a tendência tampar ou laminar ou nas formulações significadas para a liberação rápida da droga.The quantitative effects of a new gum, used as a binder, on the mechanical and release properties of paracetamol tablet formulations were analyzed in a 23 full factorial experiment. Cissus gum extracted from Cissus populnea Guill. & Perr. (Vitaceae was compared with official gelatin. The individual and interaction effects of type of binder, concentration of binder and packing fraction on the friability

  13. Efeitos antidiabÃtico, antioxidante, analgÃsico e antiinflamatÃrio da fraÃÃo solÃvel em metanol e tiramina isolados de Cissus Verticillata

    OpenAIRE

    Cleide de Sousa Lino

    2008-01-01

    IntroduÃÃo: A Cissus verticillata à uma planta, utilizada popularmente como antidiabÃtica e antiinflamatÃria, Objetivos: Investigar as atividades antidiabÃtica, analgÃsica, antiinflamatÃria e antioxidante da FraÃÃo SolÃvel em Metanol (FSM), FraÃÃo Rica em Tiramina (FRT) e tiramina (TIR) isolados de Cissus verticillata. Material e MÃtodos: Ratos Wistar, machos, foram tratados por via oral com a FSM (50 e 100 mg/kg), glibenclamida(GLI, 5 mg/kg), Ãgua destilada ou glicose), e o sangue coleta...

  14. Study of Leaf Metabolome Modifications Induced by UV-C Radiations in Representative Vitis, Cissus and Cannabis Species by LC-MS Based Metabolomics and Antioxidant Assays

    Directory of Open Access Journals (Sweden)

    Guillaume Marti

    2014-09-01

    Full Text Available UV-C radiation is known to induce metabolic modifications in plants, particularly to secondary metabolite biosynthesis. To assess these modifications from a global and untargeted perspective, the effects of the UV-C radiation of the leaves of three different model plant species, Cissus antarctica Vent. (Vitaceae, Vitis vinifera L. (Vitaceae and Cannabis sativa L. (Cannabaceae, were evaluated by an LC-HRMS-based metabolomic approach. The approach enabled the detection of significant metabolite modifications in the three species studied. For all species, clear modifications of phenylpropanoid metabolism were detected that led to an increased level of stilbene derivatives. Interestingly, resveratrol and piceid levels were strongly induced by the UV-C treatment of C. antarctica leaves. In contrast, both flavonoids and stilbene polymers were upregulated in UV-C-treated Vitis leaves. In Cannabis, important changes in cinnamic acid amides and stilbene-related compounds were also detected. Overall, our results highlighted phytoalexin induction upon UV-C radiation. To evaluate whether UV-C stress radiation could enhance the biosynthesis of bioactive compounds, the antioxidant activity of extracts from control and UV-C-treated leaves was measured. The results showed increased antioxidant activity in UV-C-treated V. vinifera extracts.

  15. Anatomical, growth and development studies in Badea fruits (Passiflora cuadrangularis Estudio anatómico y de los procesos de crecimiento y desarrollo del fruto de la badea (Passiflora quadrangularis

    Directory of Open Access Journals (Sweden)

    Montenegro de Carrillo Nohora

    1988-12-01

    Full Text Available This work had been done as a contribution to the study the processes of growth and development of Badea fruit
    (Passiflora quadrangularis L.. Fruit samples of different sizes were taken and treated by histological techniques and analyzed under microscope. Eight stage of development of
    diameter fruits were evaluated: 6; 10; 18; 22; 30; 40; 60 y 80 mm. Continues divisions in the external epidermis cells and parenchima on the three first stages were observed.
    Aditionally, the fruit originated from a triangular ovary, progresively pets a circular from. From the fourth stage, fruit ripenning initiates and the placenta starts to fill the locules and sarround the seed. At the eight stage the aril, which is originated from thickening of funiculus apical zone is observed. all the proeesses, which take place during
    the fruíts growth produce their final form and establish the organoleptic caracteristics.
    El presente trabajo se realizó para contribuir al estudio de los procesos de crecimiento y desarrollo del fruto de la Badea
    (Passif/ora quadrangularis L.. Para su realización se tomaron muestras de frutos de diferentes tamaños, los cuales fueron tratados mediante técnicas histológicas y analizados al microscopio. Se evaluaron ocho estadios de crecimiento correspondientes a frutos de los diámetros: 6; 10; 18; 22; 30; 40; 60 y 80 mm. En los tres primeros estadios de desarrollo del fruto, se observaron continuas divisiones
    en las células de la epidermis externa y del parénquima. Adicionalmente, el fruto que se ha originado de un ovario triangular, va tomando forma circular. A partir del cuarto
    estadio, se inicia la maduración del fruto y la placenta ernpiezaa llenar los lóculos y a rodear las semillas. En el estadio ocho, se observa el arilo cuyo origen proviene de un engrosamiento de la zona apical del funículo. Todos los procesos, que ocurren a lo largo del crecimiento del fruto, originan su forma final y

  16. Vegetation Composition, Biomass Production, Carrying Capacity ...

    African Journals Online (AJOL)

    Acacia tortilis, Acacia nilotica, Acacia mellifera and Acacia seyal were the most dominant shrubs with scattered Caddaba rotundifolia, Caddaba furmisa, Seddera bagshawei, Tamarix nilotica, Dobera glabra and abundant Parthenium hysterophorus, Cissus rotundifolia and C. quadrangularis. The grass biomass estimated in ...

  17. Author Details

    African Journals Online (AJOL)

    Thippeswamy, A.H.M. Vol 9, No 2 (2006) - Articles Some neuropharmacological effects of the methanolic root extract of Cissus quadrangularis in mice. Abstract PDF. ISSN: 1119-5096. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's ...

  18. African Journal of Biomedical Research - Vol 9, No 2 (2006)

    African Journals Online (AJOL)

    Some neuropharmacological effects of the methanolic root extract of Cissus quadrangularis in mice. EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. A.H.M Viswanatha Swamy, A.H.M Thippeswamy, D.V Manjula, C.B Mahendra Kumar.

  19. Averrhoa carambola L., Syzygium cumini (L. Skeels and Cissus sicyoides L.: medicinal herbal tea effects on vegetal and animal test systems Averrhoa carambola L., Syzygium cumini (L. Skeels e Cissus sicyoides L.: efeitos dos chás de plantas medicinais sobre os sistemas-teste vegetal e animal

    Directory of Open Access Journals (Sweden)

    Mario Sergio Mantovani

    2001-05-01

    Full Text Available Since folk medicine has been greatly appreciated for centuries, many researchers decided to study more deeply the curative qualities of plants. In the present study, meristematic cells of Allium cepa L. were used as vegetal test system and bone marrow cells of Wistar rats as animal test system. Both were treated in vivo to evaluate whether the plants Averrhoa carambola L., Syzygium cumini (L. Skeels and Cissus sicyoides L. presented cytotoxic and mutagenic effects and whether they resulted in cell alterations in their morphology, chromosomes or cell cycle division. Herbal teas were prepared as normally done by the population, albeit in two different concentrations, the usual concentration and a concentration ten times higher. Rats were treated with only one concentration of teas. Results showed that teas did not alter the cell cycle of Allium cepa L., with the exception of the 24 hours analysis after suspension of treatment (recovery of treatments, with a lower concentration of Averrhoa carambola. The latter had a low mitotic index when compared to control and to the post-treatment analysis, showing an inhibition of cell division. The three herbal teas neither induced an increase in the number of chromosomal damage in bone marrow cells of Wistar rats nor altered the cell division cycle. Results are important in so far as these plants are used as therapeutic agentsAs plantas medicinais têm sido muito estudadas devido aos seus efeitos curativos. Neste estudo foram utilizados o sistema teste vegetal em células meristemáticas de Allium cepa L. e o sistema teste animal em células da medula óssea de ratos Wistar tratados in vivo para avaliação dos efeitos citotóxicos e mutagênicos das plantas Averrhoa carambola L., Syzygium cumini (L. Skeels e Cissus sicyoides L., analisando-se o ciclo de divisão celular, morfologia e cromossomos. Os chás das plantas foram preparados da maneira usada pela população e em duas diferentes concentrações, a

  20. Determinação de massa fresca, massa seca, água e cinzas totais de folhas de Cissus verticillata (L. Nicolson & C. E. Jarvis subsp. verticillata e avaliação do processo de secagem em estufa com ventilação forçada

    Directory of Open Access Journals (Sweden)

    T. V. BRAGA

    2009-01-01

    Full Text Available

    Cissus verticillata (L. Nicolson & C. E. Jarvis subsp. verticillata (Vitaceae é conhecida popularmente como insulina vegetal, cortina japonesa, uva-brava, anil trepador e cipó-pucá e utilizada na medicina popular na forma de chá das folhas no tratamento da diabetes, como antiinflamatório, antiepilético, antihipertensivo, antitérmico, antireumático, antigripal e contra infecções respiratórias. O objetivo deste estudo foi avaliar os teores de massa fresca, massa seca, percentual de água e percentual de cinzas totais, visando melhoria das condições de secagem, armazenamento, dispensação e uso pela população. As folhas da espécie foram coletadas no bairro Antônio Dias, Ouro Preto, Minas Gerais, Brasil. Foram selecionadas, lavadas e secas em estufa com ventilação forçada à 45ºC e determinou-se as cinzas totais e perda por dessecação (através do método gravimétrico, ambos de acordo com a Farmacopéia Brasileira. Os resultados mostraram que as folhas de C. verticillata subsp. verticillata possuem alto teor de água. O processo de secagem em estufa de ventilação forçada, a temperatura de 45ºC, foi eficaz, proporcionando folhas com 11,47% de umidade e 17,99% de cinzas totais. Palavras-chave: Cissus verticillata; insulina vegetal; qualidade; gravimetria.

  1. TENSILE RESPONSES OF TREATED CISSUS POPULNEA FIBERS

    African Journals Online (AJOL)

    HOD

    2 DEPARTMENT OF CHEMICAL ENGINEERING, NNAMDI AZIKIWE ... (RSM) with central composite design. ... synthetic fibers for industrial applications partly ..... Cellulosic Fibers Using Eco-Friendly Compounds .... 1078 – 1097, 2017.

  2. Phytochemical Characterization And Biochemical Studies Of Cissus ...

    African Journals Online (AJOL)

    ... saponins, steroids, cardiac glycosides, lipids and vitamin E; whereas balsam, anthraquinone, tannins alkaloids, cardenolides and phlobactannin were completely absent. The aqueous extract was administered to the experimental rats at doses of 100, 200, 800, 1600, 3200, and 6400 mg/kg bodyweight for three weeks.

  3. Wound Healing Properties of Selected Plants Used in Ethnoveterinary Medicine

    Directory of Open Access Journals (Sweden)

    Amos Marume

    2017-09-01

    Full Text Available Plants have arrays of phytoconstituents that have wide ranging biological effects like antioxidant, anti-inflammatory and antimicrobial properties key in wound management. In vivo wound healing properties of ointments made of crude methanolic extracts (10% extract w/w in white soft paraffin of three plant species, Cissus quadrangularis L. (whole aerial plant parts, Adenium multiflorum Klotzsch (whole aerial plant parts and Erythrina abyssinica Lam. Ex DC. (leaves and bark used in ethnoveterinary medicine were evaluated on BALB/c female mice based on wound area changes, regular observations, healing skin's percentage crude protein content and histological examinations. White soft paraffin and 3% oxytetracycline ointment were used as negative and positive controls, respectively. Wound area changes over a 15 day period for mice treated with C. quadrangularis and A. multiflorum extract ointments were comparable to those of the positive control (oxytetracycline ointment. Wounds managed with the same extract ointments exhibited high crude protein contents, similar to what was observed on animals treated with the positive control. Histological evaluations revealed that C. quadrangularis had superior wound healing properties with the wound area completely returning to normal skin structure by day 15 of the experiment. E. abyssinica leaf and bark extract ointments exhibited lower wound healing properties though the leaf extract exhibited some modest healing properties.

  4. Tensile responses of treated Cissus populnea fibers | Azeez ...

    African Journals Online (AJOL)

    acetic acid (EDTA), respectively, were used for fiber treatment and optimi zed with variable parameters (concentration and time) using response surface methodology (RSM) with central composite design. Scanning electron microscopy (SEM) with ...

  5. Effects of diets containing Cissus rotundifolia flour on lipid profile of ...

    African Journals Online (AJOL)

    hp

    2016-04-06

    Apr 6, 2016 ... high fibre diet and polysaccharide gums in the dietary management of ... However, Guarigutata et al., (2014) reported ... powder. Previous work (Onyechi et al., 2007) on the nutrient content of CR flour per g/100 g dry weight.

  6. Ethno-veterinary control of bovine dermatophilosis and ticks in Zhombe, Njelele and Shamrock resettlement in Zimbabwe.

    Science.gov (United States)

    Ndhlovu, Daud Nyosi; Masika, Patrick J

    2013-02-01

    A structured questionnaire survey was conducted to determine the ethno-veterinary practices and other control methods used by smallholder farmers for the management of bovine dermatophilosis and ticks. A total of 153 farmers were interviewed from Njelele, Zhombe communal and Shamrock resettlement areas. Crop production contributed most to livelihoods (83.2 %) while livestock contributed 9.0 %. Over 90 % of the respondents had attended school up to primary level, with 11.4 % undergoing animal health and husbandry training. Treatment of livestock diseases was practised by 96 % of the farmers, and 49.7 % of these farmers used ethno-veterinary medicines. Across the study sites, dermatophilosis was controlled using the following plants: Cissus quadrangularis (59.7 %), Catunaregam spinosa (10.5 %), Pterocarpus angolensis (10.5 %), Kalanchoe lanceolata (5.3 %), Aloe chabaudii (3.5 %), Cassia abbreviata (1.8 %), Dichrostachys cinerea (1.8 %), Urginea sanguinea (1.8 %), Ximenia caffra (1.8 %) and a plant locally called umfanawembila (1.8 %). Carica papaya and two plants, locally called mugimbura and umdungudungu, were used for tick control, and these were reported once from Njelele communal. Other control methods, besides plants or conventional drugs, were used by 28 % of the farmers for the treatment of dermatophilosis and ticks. Some farmers (14.4 %) claimed that ethno-veterinary medicines performed better than conventional drugs. The study revealed that farmers used ethno-veterinary medical practices for the treatment of dermatophilosis but rarely for tick control.

  7. Berenty Reserve—A Gallery Forest in Decline in Dry Southern Madagascar—Towards Forest Restoration

    Directory of Open Access Journals (Sweden)

    Vanessa Winchester

    2018-01-01

    Full Text Available Berenty Reserve, a fully protected gallery forest beside the Mandrare River is renowned for its lemurs, but the continuous canopy of the main forest is shrinking, fragmenting and degrading. The aim of this study, before any restoration can be considered, is to investigate why canopy-cover is declining and define the forest’s vegetation status and composition. Our study includes analysis of tamarind age (the dominant species and regeneration, forest extent, climate and soil. Measurement of trunk circumference and annual rings indicated a median age of 190 years, near the accepted maximum for tamarinds. There is no regeneration of tamarind seedlings under the canopy and an invasive vine, Cissus quadrangularis suffocates any regeneration on the forest margins. A vegetation survey, based on fifteen transects, broadly characterized three forest areas: continuous canopy near the river, transitional canopy with fewer tall trees, and degraded dryland; the survey also provided a list of the 18 most common tree species. Ring counts of flood-damaged roots combined with measurement to the riverbank show that erosion rates, up to 19.5 cm/year, are not an immediate threat to forest extent. The highly variable climate shows no trend and analysis of forest soil indicates compatibility with plant growth.

  8. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Lans Cheryl A

    2006-10-01

    Full Text Available Abstract Background This paper is based on ethnobotanical interviews conducted from 1996–2000 in Trinidad and Tobago with thirty male and female respondents. Methods A non-experimental validation was conducted on the plants used for urinary problems and diabetes mellitus: This is a preliminary step to establish that the plants used are safe or effective, to help direct clinical trials, and to inform Caribbean physicians of the plants' known properties to avoid counter-prescribing. Results The following plants are used to treat diabetes: Antigonon leptopus, Bidens alba, Bidens pilosa, Bixa orellana, Bontia daphnoides, Carica papaya, Catharanthus roseus, Cocos nucifera, Gomphrena globosa, Laportea aestuans, Momordica charantia, Morus alba, Phyllanthus urinaria and Spiranthes acaulis. Apium graviolens is used as a heart tonic and for low blood pressure. Bixa orellana, Bontia daphnoides, Cuscuta americana and Gomphrena globosa are used for jaundice. The following plants are used for hypertension: Aloe vera, Annona muricata, Artocarpus altilis, Bixa orellana, Bidens alba, Bidens pilosa, Bonta daphnoides, Carica papaya, Cecropia peltata, Citrus paradisi, Cola nitida, Crescentia cujete, Gomphrena globosa, Hibiscus sabdariffa, Kalanchoe pinnata, Morus alba, Nopalea cochinellifera, Ocimum campechianum, Passiflora quadrangularis, Persea americana and Tamarindus indicus. The plants used for kidney problems are Theobroma cacao, Chamaesyce hirta, Flemingia strobilifera, Peperomia rotundifolia, Petiveria alliacea, Nopalea cochinellifera, Apium graveolens, Cynodon dactylon, Eleusine indica, Gomphrena globosa, Pityrogramma calomelanos and Vetiveria zizanioides. Plants are also used for gall stones and for cooling. Conclusion Chamaesyce hirta, Cissus verticillata, Kalanchoe pinnata, Peperomia spp., Portulaca oleraceae, Scoparia dulcis, and Zea mays have sufficient evidence to support their traditional use for urinary problems, "cooling" and high cholesterol

  9. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus

    Science.gov (United States)

    Lans, Cheryl A

    2006-01-01

    Background This paper is based on ethnobotanical interviews conducted from 1996–2000 in Trinidad and Tobago with thirty male and female respondents. Methods A non-experimental validation was conducted on the plants used for urinary problems and diabetes mellitus: This is a preliminary step to establish that the plants used are safe or effective, to help direct clinical trials, and to inform Caribbean physicians of the plants' known properties to avoid counter-prescribing. Results The following plants are used to treat diabetes: Antigonon leptopus, Bidens alba, Bidens pilosa, Bixa orellana, Bontia daphnoides, Carica papaya, Catharanthus roseus, Cocos nucifera, Gomphrena globosa, Laportea aestuans, Momordica charantia, Morus alba, Phyllanthus urinaria and Spiranthes acaulis. Apium graviolens is used as a heart tonic and for low blood pressure. Bixa orellana, Bontia daphnoides, Cuscuta americana and Gomphrena globosa are used for jaundice. The following plants are used for hypertension: Aloe vera, Annona muricata, Artocarpus altilis, Bixa orellana, Bidens alba, Bidens pilosa, Bonta daphnoides, Carica papaya, Cecropia peltata, Citrus paradisi, Cola nitida, Crescentia cujete, Gomphrena globosa, Hibiscus sabdariffa, Kalanchoe pinnata, Morus alba, Nopalea cochinellifera, Ocimum campechianum, Passiflora quadrangularis, Persea americana and Tamarindus indicus. The plants used for kidney problems are Theobroma cacao, Chamaesyce hirta, Flemingia strobilifera, Peperomia rotundifolia, Petiveria alliacea, Nopalea cochinellifera, Apium graveolens, Cynodon dactylon, Eleusine indica, Gomphrena globosa, Pityrogramma calomelanos and Vetiveria zizanioides. Plants are also used for gall stones and for cooling. Conclusion Chamaesyce hirta, Cissus verticillata, Kalanchoe pinnata, Peperomia spp., Portulaca oleraceae, Scoparia dulcis, and Zea mays have sufficient evidence to support their traditional use for urinary problems, "cooling" and high cholesterol. Eggplant extract as a

  10. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus.

    Science.gov (United States)

    Lans, Cheryl A

    2006-10-13

    This paper is based on ethnobotanical interviews conducted from 1996-2000 in Trinidad and Tobago with thirty male and female respondents. A non-experimental validation was conducted on the plants used for urinary problems and diabetes mellitus: This is a preliminary step to establish that the plants used are safe or effective, to help direct clinical trials, and to inform Caribbean physicians of the plants' known properties to avoid counter-prescribing. The following plants are used to treat diabetes: Antigonon leptopus, Bidens alba, Bidens pilosa, Bixa orellana, Bontia daphnoides, Carica papaya, Catharanthus roseus, Cocos nucifera, Gomphrena globosa, Laportea aestuans, Momordica charantia, Morus alba, Phyllanthus urinaria and Spiranthes acaulis. Apium graviolens is used as a heart tonic and for low blood pressure. Bixa orellana, Bontia daphnoides, Cuscuta americana and Gomphrena globosa are used for jaundice. The following plants are used for hypertension: Aloe vera, Annona muricata, Artocarpus altilis, Bixa orellana, Bidens alba, Bidens pilosa, Bonta daphnoides, Carica papaya, Cecropia peltata, Citrus paradisi, Cola nitida, Crescentia cujete, Gomphrena globosa, Hibiscus sabdariffa, Kalanchoe pinnata, Morus alba, Nopalea cochinellifera, Ocimum campechianum, Passiflora quadrangularis, Persea americana and Tamarindus indicus. The plants used for kidney problems are Theobroma cacao, Chamaesyce hirta, Flemingia strobilifera, Peperomia rotundifolia, Petiveria alliacea, Nopalea cochinellifera, Apium graveolens, Cynodon dactylon, Eleusine indica, Gomphrena globosa, Pityrogramma calomelanos and Vetiveria zizanioides. Plants are also used for gall stones and for cooling. Chamaesyce hirta, Cissus verticillata, Kalanchoe pinnata, Peperomia spp., Portulaca oleraceae, Scoparia dulcis, and Zea mays have sufficient evidence to support their traditional use for urinary problems, "cooling" and high cholesterol. Eggplant extract as a hypocholesterolemic agent has some support but

  11. Physical, sensory and chemical properties of bread prepared from ...

    African Journals Online (AJOL)

    Physical, sensory and chemical properties of bread prepared from wheat and ... Different levels (0, 1, 2 and 3% w/w) of cissus gum powder was added to ... flours for bread making where 100% wheat bread without cissus gum served as control. ... serve as a gluten substitute in preparing acceptable wheat bread substituted ...

  12. Phytochemical Screening and Preliminary Evaluation of Analgesic ...

    African Journals Online (AJOL)

    In this study, the methanolic root extract of Cissus polyantha was subjected to preliminary phytochemical screening, analgesic and anti-inflammatory studies. Phytochemical studies was carried out using standard phytochemical protocol while the analgesic studies was carried out using acetic acid-induced writhing tests in ...

  13. Browse Title Index

    African Journals Online (AJOL)

    Items 701 - 745 of 745 ... Vol 8, No 4 (1998), Topical fluorides and caries prevention in Nigeria, Abstract. E O Sote. Vol 10, No 3 (2000), Toxicity test of water homogenate of cissus ... Vol 13, No 1 (2003), Trends in dental treatment of children at ...

  14. African Journal of Biotechnology - Vol 15, No 14 (2016)

    African Journals Online (AJOL)

    Ibrahim M. Farag, Ahmed M. Darwish, Hassan R. Darwish, K. B AbdelAziz, W. A Ramadan, M.I. Mohamed, Othman E. Othman, 549-556 ... Effects of diets containing Cissus rotundifolia flour on lipid profile of rats and postprandial glucose levels of normoglycemic human adults · EMAIL FREE FULL TEXT EMAIL FREE FULL ...

  15. Author Details

    African Journals Online (AJOL)

    Famojuro, TI. Vol 19 (2015) - Articles Survey of Medicinal Plants Used in the Management of Sickle Cell Disease by Traditional Medical Practitioners of Gbonyin Local Government Area of Ekiti State, Nigeria Abstract · Vol 19 (2015) - Articles Antisickling Activity of the Fresh and Dried Roots of Cissus populnea Guill. Et Perr ...

  16. Local perception and proximate analysis of some edible forest plants

    African Journals Online (AJOL)

    User

    The Focus Group. Discussion (FGD) technique was employed to obtain information from local residents in four ... 0 and 0.13 ± 0.07), fibre and ash content were highest in Cissus populnea (29.37 ± 0.41 and ..... Agricultural Systems. Pp 1-14.

  17. Antiproliferative effect of alcoholic extracts of some Gabonese ...

    African Journals Online (AJOL)

    Extracts from Piptadeniastrum africanum Brenan (Mimosaceae), Petersianthus macrocarpus (Breauv) L. (Lecydaceae), Cissus debilis Planch (Vitaceae) and Dieffenbachia seguine Jacq. (Araceae) were tested in vitro for their antiproliferative activity on human colon cancer cell line (CaCo-2). The highest antiproliferative ...

  18. Megafauna of vulnerable marine ecosystems in French mediterranean submarine canyons: Spatial distribution and anthropogenic impacts

    OpenAIRE

    Fabri, Marie-claire; Pedel, Laura; Beuck, L.; Galgani, Francois; Hebbeln, D.; Freiwald, A.

    2014-01-01

    Vulnerable Marine Ecosystems (VME) in the deep Mediterranean Sea have been identified by the General Fisheries Commission for the Mediterranean as consisting of communities of Scleractinia (Lophelia pertusa and Madrepora oculata), Pennatulacea (Funiculina quadrangularis) and Alcyonacea (Isidella elongata). This paper deals with video data recorded in the heads of French Mediterranean canyons. Quantitative observations were extracted from 101 video films recorded during the MEDSEACAN cruise in...

  19. Drug Development and Conservation of Biodiversity in West and Central Africa: Performance of Neurochemical and Radio Receptor Assays of Plant Extracts Drug Discovery for the Central Nervous System

    Science.gov (United States)

    2004-09-01

    Erythrophleum guineensis 1909 MeOH --/38 --/52 P. nitida SU1905 MeOH --/50 4 Scoparia dulcis (whole plant) SU1912 MeOH 10 --/64 182... Scoparia dulcis SU 1913 CH2Cl2 12 11 --/74 Triumfetta tomentosa SU Aneilema umbrosum (whole plant) SU2302 CH2Cl2 10/45 --/77... Scoparia dulcis and Cissus quandrangularis. Studies of P. zenkeri were initiated because this plant is used by cattle herders in parts of Cameroon as

  20. phytochemical and antimicrobial properties of commiphora

    African Journals Online (AJOL)

    DR. AMINU

    2014-06-01

    Jun 1, 2014 ... CIPIH Study Nine on TM, Draft Report. 1-172. Sani, Y.M., Musa, A.M., Yaro, A.H., Sani, M.B.,. Amoley, A. and Magaji, M.G. (2013). Phytochemical screening and evaluation of analgesic and anti inflammatory activities of the Methanol Leaf Extract of Cissus polyantha. J. Med. Sci., 1682-4474. Sibanda T. and ...

  1. Comparative Neuropharmacological Activities Methanolic Extracts of ...

    African Journals Online (AJOL)

    Comparative neuropharmacological efficacy of the leaf and root 70 % methanol extract of Cissus cornifolia was studied in mice. The extractive values of the leaf and root methanol extract was found to be 31.5 g with yield of 12.6 %(w/w) and 37.8 g with the yield of 15.12 %(w/w) respectively. The acute toxicity (LD50) values ...

  2. Phylogenetic analysis of the grape family (Vitaceae) based on three chloroplast markers.

    Science.gov (United States)

    Soejima, Akiko; Wen, Jun

    2006-02-01

    Seventy-nine species representing 12 genera of Vitaceae were sequenced for the trnL-F spacer, 37 of which were subsequently sequenced for the atpB-rbcL spacer and the rps16 intron. Phylogenetic analysis of the combined data provided a fairly robust phylogeny for Vitaceae. Cayratia, Tetrastigma, and Cyphostemma form a clade. Cyphostemma and Tetrastigma are each monophyletic, and Cayratia may be paraphyletic. Ampelopsis is paraphyletic with the African Rhoicissus and the South American Cissus striata nested within it. The pinnately leaved Ampelopsis form a subclade, and the simple and palmately leaved Ameplopsis constitutes another with both subclades containing Asian and American species. Species of Cissus from Asia and Central America are monophyletic, but the South American C. striata does not group with other Cissus species. The Asian endemic Nothocissus and Pterisanthes form a clade with Asian Ampelocissus, and A. javalensis from Central America is sister to this clade. Vitis is monophyletic and forms a larger clade with Ampelocissus, Pterisanthes, and Nothocissus. The eastern Asian and North American disjunct Parthenocissus forms a clade with Yua austro-orientalis, a species of a small newly recognized genus from China to eastern Himalaya. Vitaceae show complex multiple intercontinental relationships within the northern hemisphere and between northern and southern hemispheres.

  3. Características del fruto de cuatro pasifloras de la zona andina venezolana

    OpenAIRE

    Aular, Jesús; Rodríguez, Yesenia; Roa, Sara; Iade, Patricia; Antolínez, María

    2004-01-01

    En la zona andina de Venezuela existen especies del género Passiflora con elevado potencial agronómico. Con el objetivo de contribuir a su conocimiento, se estudiaron las características del fruto de P. ligularis Juss, P. mollisima HBK Bailey, P. mixta L. y P. quadrangularis L. colectadas en los estados Táchira, las tres primeras especies, y Mérida, la cuarta. Se empleó un diseño estadístico completamente al azar y se determinaron características físicas y químicas de la parte comestible del ...

  4. Techos verdes y el confort térmico en Angostillo, Veracruz, México.

    OpenAIRE

    Beltrán Melgarejo, Abraham

    2013-01-01

    Se diseñó un prototipo de techo verde con enredaderas tropicales y se evaluó el confort térmico brindado por éste en viviendas de una zona rural tropical. Con base en entrevistas a viveristas de la región centro de Veracruz y un recorrido de campo, se preseleccionaron cinco especies Mandevilla sanderi, Solandra maxima, Passiflora edulis, Thunbergia alata y Cissus verticillata. Se evaluó la adaptabilidad de éstas al ambiente sobre techos modelo en condiciones de campo, midiendo sus tasas de cr...

  5. Species distribution models of two critically endangered deep-sea octocorals reveal fishing impacts on vulnerable marine ecosystems in central Mediterranean Sea.

    Science.gov (United States)

    Lauria, V; Garofalo, G; Fiorentino, F; Massi, D; Milisenda, G; Piraino, S; Russo, T; Gristina, M

    2017-08-14

    Deep-sea coral assemblages are key components of marine ecosystems that generate habitats for fish and invertebrate communities and act as marine biodiversity hot spots. Because of their life history traits, deep-sea corals are highly vulnerable to human impacts such as fishing. They are an indicator of vulnerable marine ecosystems (VMEs), therefore their conservation is essential to preserve marine biodiversity. In the Mediterranean Sea deep-sea coral habitats are associated with commercially important crustaceans, consequently their abundance has dramatically declined due to the effects of trawling. Marine spatial planning is required to ensure that the conservation of these habitats is achieved. Species distribution models were used to investigate the distribution of two critically endangered octocorals (Funiculina quadrangularis and Isidella elongata) in the central Mediterranean as a function of environmental and fisheries variables. Results show that both species exhibit species-specific habitat preferences and spatial patterns in response to environmental variables, but the impact of trawling on their distribution differed. In particular F. quadrangularis can overlap with fishing activities, whereas I. elongata occurs exclusively where fishing is low or absent. This study represents the first attempt to identify key areas for the protection of soft and compact mud VMEs in the central Mediterranean Sea.

  6. O gênero Piptocarpha R.Br. (Asteraceae: Vernonieae no estado do Paraná, Brasil The genus Piptocarpha R.Br. (Asteraceae: Vernonieae in the Paraná state, Brazil

    Directory of Open Access Journals (Sweden)

    Larissa Grokoviski

    2009-06-01

    Full Text Available O presente estudo abrange o gênero Piptocarpha R.Br. (Vernonieae: Asteraceae no Estado do Paraná, Brasil. A metodologia consistiu no levantamento bibliográfico, coleta de material e observação de campo, estudos morfológicos e taxonômicos do material vivo e herborizado. Foram confirmadas para o Estado do Paraná nove espécies, sendo quatro escandentes: Piptocarpha leprosa (Less. Baker, P. oblonga (Gardner Baker, P. quadrangularis (Vell. Baker e P. sellowii (Sch.Bip. Baker; e cinco arbóreas: Piptocarpha angustifolia Dusén ex Malme, P. axillaris (Less. Baker, P. densifolia Dusén ex G.L. Smith, P. macropoda (DC. Baker e P. regnellii (Sch.Bip. Cabrera. São apresentadas chave de identificação, descrições morfológicas, época de floração e frutificação, nomes populares, distribuição geográfica, comentários e ilustrações para cada táxon estudado.The present study analysed the genus Piptocarpha R.Br. (Vernonieae: Asteraceae in the State of Paraná, Brazil. The methodology consisted of examining literature, botanical collections and field observations, morphological and taxonomic study of herborized specimens. Nine species were confirmed, four of these are scandent species: Piptocarpha leprosa (Less. Baker, P. oblonga (Gardner Baker, P. quadrangularis (Vell. Baker and P. sellowii (Sch.Bip. Baker; and five are trees species: Piptocarpha angustifolia Dusén ex Malme, P. axillaris (Less. Baker, P. densifolia Dusén ex G.L. Smith, P. macropoda (DC. Baker and P. regnellii (Sch.Bip. Cabrera. Identification key, descriptions, flowering and fruiting time, popular names, geographical distribution, comments and illustrations are provided for each studied taxa.

  7. INVASIVE WEEDS IN BOGOR BOTANIC GARDENS, INDONESIA AND ITS IMPLICATION ON SURROUNDING LANDSCAPES

    Directory of Open Access Journals (Sweden)

    Edi Santosa

    2014-07-01

    Full Text Available Conservation areas with the objective for collection and exchange plant materials have been speculated as weed bank for surrounding areas. Objective of this study was to identify and characterize ruderal invasive weeds in the Bogor Botanic Gardens (BBG. Observations were conducted in all vak (collection blocks in the BBG in order to identify the weeds species, determine their invasiveness, dominance and distribution. Weeds associations with host plants were observed. Current weed control program and data of dead trees collection were analyzed in relevant to weed. Distribution of weeds outside BBG was observed by transects method following river and road directions. Results showed that there were seven invasive weeds, i.e., Cecropia adenopus (Cecropiaceae, Cissus nodosa Blume (Vitaceae, Cissus sicyoides Blume (Vitaceae, Dioscorea bulbifera L. (Dioscoreaceae, Ficus elastica Roxb. (Moraceae, Mikania micrantha H.B.K. (Asteraceae and Paraserianthes falcataria (L. Nielsen (Fabaceae. These seven weeds species invaded 41 out of 215 plant families in BBG. Six species of weeds, i.e., C. adenopus, C. nodosa Blume., C. sicyoides Blume., D. bulbifera L., M. micrantha H.B.K. and P. falcataria (L. Nielsen, were introduced as BBG collections for the first time while the F. elastica Roxb was considered as native. It is most likely that the weeds dispersal agents are the wind, birds, bats, visitors, and waters. All of these weeds existed in surrounding areas outside BBG. Given the detrimental impact of invasive weeds on the plant collection in BBG, it is necessary to develop long–term comprehensive control measures both inside and neighboring areas by involving other government authorities beyond BBG.

  8. Megafauna of vulnerable marine ecosystems in French mediterranean submarine canyons: Spatial distribution and anthropogenic impacts

    Science.gov (United States)

    Fabri, M.-C.; Pedel, L.; Beuck, L.; Galgani, F.; Hebbeln, D.; Freiwald, A.

    2014-06-01

    Vulnerable Marine Ecosystems (VME) in the deep Mediterranean Sea have been identified by the General Fisheries Commission for the Mediterranean as consisting of communities of Scleractinia (Lophelia pertusa and Madrepora oculata), Pennatulacea (Funiculina quadrangularis) and Alcyonacea (Isidella elongata). This paper deals with video data recorded in the heads of French Mediterranean canyons. Quantitative observations were extracted from 101 video films recorded during the MEDSEACAN cruise in 2009 (Aamp/Comex). Qualitative information was extracted from four other cruises (two Marum/Comex cruises in 2009 and 2011 and two Ifremer cruises in 1995 and 2010) to support the previous observations in the Cassidaigne and Lacaze-Duthiers canyons. All the species, fishing impacts and litter recognized in the video films recorded from 180 to 700 m depth were mapped using GIS. The abundances and distributions of benthic fishing resources (marketable fishes, Aristeidae, Octopodidae), Vulnerable Marine Species, trawling scars and litter of 17 canyons were calculated and compared, as was the open slope between the Stoechades and Toulon canyons. Funiculina quadrangularis was rarely observed, being confined for the most part to the Marti canyon and, I. elongata was abundant in three canyons (Bourcart, Marti, Petit-Rhône). These two cnidarians were encountered in relatively low abundances, and it may be that they have been swept away by repeated trawling. The Lacaze-Duthiers and Cassidaigne canyons comprised the highest densities and largest colony sizes of scleractinian cold-water corals, whose distribution was mapped in detail. These colonies were often seen to be entangled in fishing lines. The alcyonacean Callogorgia verticillata was observed to be highly abundant in the Bourcart canyon and less abundant in several other canyons. This alcyonacean was also severely affected by bottom fishing gears and is proposed as a Vulnerable Marine Species. Our studies on anthropogenic

  9. Aspectos vegetativos de combinações copa/porta-enxerto em maracujazeiro

    Directory of Open Access Journals (Sweden)

    Givanildo Roncatto

    2011-09-01

    Full Text Available O maracujazeiro tem-se destacado entre as principais frutíferas do País. Porém, a vida útil vem sendo reduzida principalmente devido aos danos causados por doenças do sistema radicular, sendo que a enxertia com espécies nativas e resistentes a doenças pode solucionar o problema. Com isso, objetivou-se avaliar o desempenho vegetativo das mudas enxertadas de combinações de variedades-copa e espécies de porta-enxertos de maracujazeiro. O trabalho foi desenvolvido em viveiro telado (50% de sombreamento, na Embrapa/Acre, em Rio Branco-AC, entre setembro e dezembro de 2007. As sete variedades estudadas foram o maracujazeiro-amarelo 'FB 100' e 'FB 200' do viveiro Flora Brasil (Araguari-MG, UFAC 07; 25; 38; 64 e 70 (Universidade Federal do Acre, Rio Branco-AC, e os porta-enxertos foram Passiflora edulis f. flavicarpa (acesso Cuiabá-MT, P. alata, P. edulis e P. quadrangularis (acesso Guiratinga-MT, P. serrato-digitata (acesso IAC-Campinas-SP. O delineamento experimental foi o de blocos ao caso, com 12 repetições. Os tratamentos foram 35 combinações copa/porta-enxerto constituídas por 5 portas-enxertos combinados com 7 copas. O método de enxertia utilizado foi o de fenda cheia no topo hipocotiledonar, tendo os porta-enxertos as seguintes características: três folhas verdadeiras e altura de plântula variável (6 a 8cm, 30 a 90 dias após a semeadura. Foram avaliadas a altura de plantas, o diâmetro do caule, o número de folhas e de entrenós como valores de desenvolvimento das plantas. As combinações de melhor desenvolvimento vegetativo para o diâmetro e o número de entrenós foram FB 100 e FB 200 sobre P. edulis e P. alata, UFAC 07 sobre P. serrato-digitata e P. quadrangularis, UFAC 38 sobre P. edulis. Já para a altura de plantas e o número de folhas, as combinações de melhor desenvolvimento vegetativo foram FB 100 e FB 200 sobre P. edulis e P. alata, bem como FB 100 e FB 200 sobre P. serrato-digitata, UFAC 38 sobre P. alata

  10. Enxertia de progênies de maracujazeiro-roxo australiano em espécies nativas Grafting of australian purple passion fruit progenies in different native species

    Directory of Open Access Journals (Sweden)

    Márcio de Carvalho Pires

    2009-09-01

    Full Text Available O maracujá-roxo (Passiflora edulis, apesar de ser desconhecido no Brasil, pode tornar-se numa fonte de renda alternativa para o agricultor, devido à boa remuneração e aceitação da fruta in natura no mercado europeu. Com o objetivo de avaliar a produção de mudas clonais de maracujá-roxo provenientes da Austrália, foi realizado um experimento no Setor de Fruticultura da Universidade de Brasília entre os meses de maio de 2005 e fevereiro de 2006. O delineamento experimental utilizado foi o de blocos casualizados, com 3 repetições, em arranjo fatorial 7 x 2 x 3, sendo sete porta-enxertos, duas variedades-copa e três épocas de avaliação. A parcela foi formada por 3 plantas úteis. Utilizaram-se como porta-enxertos (PE as espécies P. serrato digitata, P. nitida, P. coccinea, P. quadrangularis, P. edulis e P. edulis f. flavicarpa híbrido 'EC-2-0' e o híbrido P. coccinea X P. setacea, e como copa (CP, as variedades '96A' e '25' de maracujazeiro-roxo. As estacas enraizadas foram transferidas para sacolas plásticas e mantidas sob nebulização intermitente. A enxertia foi efetuada 30 dias após. As avaliações do índice de pegamento foram efetuadas aos 31 e 61 dias após a enxertia (DAE. As combinações CP/PE (entre "25" e "96 A" X P. nitida, seguidas das combinações ("25" e "96 A" X P. edulis f. flavicarpa híbrido 'EC-2-0', com 100%, 90%, 90% e 80%, respectivamente, obtiveram alto índice de pegamento aos 61 DAE. A produção de mudas de maracujazeiro-roxo enxertadas nas espécies de maracujazeiro silvestres e comerciais P. nitida, P. quadrangularis, P. edulis f. flavicarpa híbrido 'EC-2-0' e P. edulis é viável do ponto de vista técnico, pois não ocorreram problemas de incompatibilidade, e os enxertos apresentaram alta taxa de pegamento.Purple passion fruit (Passiflora edulis, even though is unknown in Brazil, can be an alternative income for the farmers due to good price and acceptance by the European market. Aiming to

  11. Chemical composition of hydroethanolic extracts from five species of the Passiflora genus

    Directory of Open Access Journals (Sweden)

    Marna E Sakalem

    2012-09-01

    Full Text Available The diversified genus Passiflora is well distributed all over Brazil, and many species have been long used as medicinal plants, mainly against anxiety disturbances. This effect has been attributed to its rich flavonoid composition. Flavonoids’ main class, flavonoid glycosides, has presented central action, particularly as sedative-hypnotic, anxiolytic and analgesic. The objective of the present study was to make a phytochemical screening of five little studied Passiflora species, in order to evaluate their phenolic composition. For this aim, HPLC-DAD-ESI-MS/MS was used. After the preparation of the hydroalcoholic extracts, each species was evaluated by direct injection electrospray ionization (ESI and tandem mass spectrometry. Although belonging to the same genus, the composition of each species presented particularities; this justifies the importance of studies aiming for the phenolic composition of different Passiflora species. Flavones C-glycosides were detected in all extracts, and are found as the main constituents in P. vitifolia, P. coccinea, P. bahiensis and P. sidifolia. In this last one, flavone-6,8-di-C-glycoside, apigenin-6-C-rhamnosyl-8-C-arabinoside are present in high content. Cyclopassiflosides were found in high content together with cyanogenic glycosides in P. quadrangularis, while in P. coccinea, besides flavones-C-glycosides were also found procyanidins.

  12. Chemical composition of hydroethanolic extracts from five species of the Passiflora genus

    Directory of Open Access Journals (Sweden)

    Marna E Sakalem

    2012-12-01

    Full Text Available The diversified genus Passiflora is well distributed all over Brazil, and many species have been long used as medicinal plants, mainly against anxiety disturbances. This effect has been attributed to its rich flavonoid composition. Flavonoids’ main class, flavonoid glycosides, has presented central action, particularly as sedative-hypnotic, anxiolytic and analgesic. The objective of the present study was to make a phytochemical screening of five little studied Passiflora species, in order to evaluate their phenolic composition. For this aim, HPLC-DAD-ESI-MS/MS was used. After the preparation of the hydroalcoholic extracts, each species was evaluated by direct injection electrospray ionization (ESI and tandem mass spectrometry. Although belonging to the same genus, the composition of each species presented particularities; this justifies the importance of studies aiming for the phenolic composition of different Passiflora species. Flavones C-glycosides were detected in all extracts, and are found as the main constituents in P. vitifolia, P. coccinea, P. bahiensis and P. sidifolia. In this last one, flavone-6,8-di-C-glycoside, apigenin-6-C-rhamnosyl-8-C-arabinoside are present in high content. Cyclopassiflosides were found in high content together with cyanogenic glycosides in P. quadrangularis, while in P. coccinea, besides flavones-C-glycosides were also found procyanidins.

  13. Are stem nectaries common in Gentianaceae Juss.?

    Directory of Open Access Journals (Sweden)

    Valdnéa Casagrande Dalvi

    Full Text Available ABSTRACT Extrafloral nectaries (EFNs are specialized structures that produce and release nectar and are located on leaves, cotyledons and, more seldomly, stems. Peculiar leaf nectaries have been described for thirty-three Neotropical species of Gentianaceae, while stem nectaries have been reported for only nine. The aim of this study was to verify the occurrence of stem EFNs within Gentianaceae and investigate the existence of a correlation between their occurrence and the geographical distribution of species. Samples of internodal regions from field and herbarium specimens were submitted to standard light microscopy techniques. Data regarding the geographical distribution of species were acquired from herbarium specimens and the literature. A total of 37 species were investigated, representing 25 genera distributed among five tribes. Nectaries, composed of modified epidermal cells, were observed in 16 species restricted to the Neotropical tribes Helieae, Saccifolieae, Potalieae and Coutoubeinae; exceptions were Cicendia quadrangularis and Zygostigma australe, which both occur in the Neotropics but do not possess EFNs. These results demonstrate that stem EFNs are common among Neotropical taxa of Gentianaceae, and are typically absent from taxa in temperate regions.

  14. Preliminary screening of some traditional Zulu medicinal plants for antineoplastic activities versus the HepG2 cell line.

    Science.gov (United States)

    Opoku, A R; Geheeb-Keller, M; Lin, J; Terblanche, S E; Hutchings, A; Chuturgoon, A; Pillay, D

    2000-11-01

    Aqueous and methanol extracts of nine traditional Zulu medicinal plants, Cissus quandrangularis L., Cyphostemma flaviflorum (Sprague) Descoings, Cyphostemma lanigerum (Harv.) Descoings ex Wild & Drum, Cyphostemma natalitium (Szyszyl.) J. v. d. Merwe, Cyphostemma sp., Rhoicissus digitata (L. F.) Gilg & Brandt, Rhoicissus rhomboidea (E. Mey. Ex harv.) Planch, Rhoicissus tomentosa (Lam.) Wild & Drum, R. tridentata (L. F.) Wild & Drum and Rhoicissus tridentata (L. F.) Wild & Drum subsp. cuneifolia (Eckl. & Zeyh.) N. R. Urton, all belonging to the Vitaceae family, were evaluated to determine their therapeutic potentials as antineoplastic agents. The antiproliferative activity in vitro against HepG2 cells was determined. Twenty-two of the twenty-seven crude plant extracts showed activities ranging from 25% to 97% inhibition of proliferation when compared with the control which showed no inhibitory activity. Higher degrees of growth inhibition were found in aqueous root extracts in comparison with the methanol extracts of the same plant parts. The results show potential antineoplastic activity, indicating some scientific validation for traditional usage. Copyright 2000 John Wiley & Sons, Ltd.

  15. NUTRITIONAL AND ANTINUTRITIONAL EVALUATION OF SOME UNCONVENTIONAL WILD EDIBLE PLANTS

    Directory of Open Access Journals (Sweden)

    Veerabahu Ramasamy Mohan

    2010-03-01

    Full Text Available The wild edible tubers, rhizome, corm, roots and stems were consumed by the tribal Valaiyans of Madurai district, Western Ghats, Tamil Nadu were analysed for proximate and mineral composition, starch, vitamins, in vitro protein (IVPD, in vitro starch (IVSD digestibility and certain antinutritional factors. The tubers of Kedrostis foetidissima and stem of Caralluma pauciflora contain higher contents of crude protein. The tubers of Decalepis hamiltonii and stems of Caralluma adscendens var attenuata and C. pauciflora contain higher contents of crude lipids. All the presently investigated wild edible plants appeared to have a higher level of iron content compared to Recommended Dietary Allowances (RDA of NRC/NAS (1980 for infants, children and adults. The tubers of Cissus vitiginea, Dioscorea pentaphylla var. pentaphylla, D. oppositifolia var. oppositifolia, D. spicata, D. tomentosa, Kedrostis foetidissima, Parthenocissus neilgherriensis, in the corm of Colocasia esculenta, in the rhizome of Canna indica and in the root of Ipomoea staphylina were formed to contain more starch. The tubers of Cycas circinalis, Cyphostemma setosum, D. oppositifolia var. oppositifolia, Dioscorea pentaphylla var. pentaphylla, Kedrostis foetidissima, Parthenocissus neilgherriensis, and in the stem of Caralluma pauciflora were found to be higher niacin content. All the investigated samples in vitro protein digestibility (IVPD was found to be low. Antinutritional substances like total free phenolics, tannins, hydrogen cyanide, total oxalate, amylase and trypsin inhibitor activity were also investigated.

  16. Variabilidade genética de acessos obtidos de populações cultivadas e silvestres de maracujazeiro-doce com base em marcadores rapd Genetic diversity obtained from cultivated population and native accesses of seewt passion fruit based on rapd markers

    Directory of Open Access Journals (Sweden)

    Graciele Bellon

    2009-03-01

    Full Text Available O maracujazeiro-doce (Passiflora alata Curtis, devido a preços diferenciados, vem ganhando importância dentro do mercado de frutas in natura. O melhoramento genético é fundamental para elevar a qualidade e a produtividade da cultura. Os marcadores moleculares do DNA têm sido muito úteis por permitirem a obtenção de um número praticamente ilimitado de polimorfismo genético sem influência do ambiente. Objetivou-se, neste trabalho, estudar a variabilidade genética de 17 acessos de maracujá-doce, com base em marcadores moleculares RAPD. Um acesso de P. quadrangularis e um de P. edulis foram utilizados como outgroups. Amostras de DNA genômico de cada acesso foram extraídas e 11 iniciadores decâmeros (OPD 04; 07; 08 e16; OPE 18 e 20; OPF 01 e 14; OPG 08; OPH 12 e 16 foram utilizados para a obtenção dos marcadores. Os marcadores obtidos foram convertidos em uma matriz de dados binários, a partir da qual foram estimadas as distâncias genéticas entre os acessos e realizadas análises de agrupamento e de dispersão gráfica. Do total de marcadores, considerando-se apenas os acessos de P. alata, observaram-se 87 (62,12% bandas polimórficas, evidenciando a grande variabilidade intraespecífica. A análise de agrupamento realizada com base nas distâncias genéticas permitiu subdividir os 17 acessos de P. alata em, pelo menos, cinco grupos de similaridade genética. Os acessos silvestres foram os que mais contribuíram para a ampliação da base genética dos materiais estudados, abrindo perspectivas para o uso desses materiais em programas de melhoramento.Sweet passion fruit (Passiflora alata Curtis is gaining importance in the in natura fruit market due to differential value. Genetic breeding is crucial to improve crop quality and productivity. Molecular markers of DNA have been very useful by allowing obtaining a virtually unlimited number of genetic polymorphism without environment influence. This work's objective was to study the

  17. Chemical Constituents and Structural Characterization of Polysaccharides from Four Typical Bamboo Species Leaves

    Directory of Open Access Journals (Sweden)

    Cheng-Zhang Wang

    2015-03-01

    Full Text Available In order to find bamboo leaves with high contents of bioactive polysaccharides, 32 samples were chosen to analyze their polysaccharide content by GC and sulfuric acid-anthrone colorimetric assays. Purified polysaccharides (BLPS were separated from the four varieties P. nigra (Lodd. Munro (PN, P. vivax McClure (PV, Chimonobambusa quadrangularis (Fenzi Makino (CQ, and P. bambussoides cv. Tanakae (PB by ultrasound extraction, solution precipitation, ion exchange resin, DEAE-52 and Sephadex G-100 chromatography. BLPS structural characterization was accomplished by HPLC-GPC, Fourier transform infra-red spectroscopy (FTIR and NaIO4-HIO4 oxidation reactions. The results showed that the total polysaccharides of the bamboo leaves in samples 1–32 ranged between 1.4% and 5.4%, Samples No. 29–No. 32 (PN, PV, CQ, and PB contained 2–3 fold more polysaccharides than No. 1~No. 28 among the 32 different species, particularly the content of galactose was in a range of 21.5%–34.1% for these four typical bamboo species leaves, which was also more than 2–3 fold higher than in No. 1–No. 28. Sugar analysis indicated that PN-PBLPS-1, PV-PBLPS-1, CQ-PBLPS-1 and PB-PBLPS-1 from the four varieties were homogeneous polysaccharides with molecular weights of 2.04 × 104, 1.15 × 104, 8.75 × 104 and 1.48 × 104 Da, respectively. PB-PBLPS-1 was a mixture of α-galactopyranose and β-d-glucopyranose linkages with α-(1→6 or β-(1→6glycosidic bonds, while PN-PBLPS-1, PV-PBLPS-1, and CQ-PBLPS-1 had α galactopyranose linkages with α-(1→6 glycosidic bonds.

  18. Grapefruit as a host for the West Indian fruit fly (Diptera: Tephritidae).

    Science.gov (United States)

    Mangan, Robert L; Thomas, Donald B; Moreno, Aleena Tarshis; Robacker, David

    2011-02-01

    The most common hosts for the West Indian fruit fly, Anastrepha obliqua (Macquart) (Diptera: Tephritidae) are fruit in the family Anacardiaceae (mango [Mangifera L.] and mombin [Spondias L.] species). However, similar to many of the tropical fruit flies of major economic importance, this species attacks several other families of crop fruit, including Annonaceae (cherimoya, Annona cherimola Mill.), Myrtaceae (guava, Psidium L.), Oxalidaceae (carambola, Averrhoa carambola L.), Passifloraceae (granadilla, Passiflora quadrangularis Mill.), and Sapotaceae [mamey sapote, Pouteria sapota (Jacq.) H. E. Moore & Steam]. In the family Rutaceae the economically important genus Citrus has been reported and until recently considered a host for this fruit fly. In this study, we reviewed the taxonomy of A. obliqua, tested specific chemicals that may inhibit oviposition, compared egg-to-adult survival of A. obliqua on preferred hosts and on grapefruit (Citrus X paradisi Macfad.), and measured fruit tissue-specific developmental rates of A. obliqua and the known citrus breeding Mexican fruit fly, Anastrepha ludens (Loew) (Diptera: Tephritidae), from egg to pupae. Our literature review shows much confusion concerning the taxonomy of this and related Anastrepha species, including synonymies and confusion with other species. The deterrent effect of the highest concentration of flavonoids for oviposition, although significant, was not absolute. Experiments carried out under laboratory conditions showed 15-40 times greater survival of A. ludens (whose preferred hosts include Rutaceae) on grapefruit compared with A. obliqua for both tree attached and harvested fruit. Experiments of survival of developing stages over time showed that the two species oviposit into different tissues in the fruit, and mortality is much higher for the West Indian fruit fly in the flavedo and albedo of the fruit compared with the Mexican fruit fly.

  19. Snakebites and ethnobotany in the northwest region of Colombia. Part III: neutralization of the haemorrhagic effect of Bothrops atrox venom.

    Science.gov (United States)

    Otero, R; Núñez, V; Barona, J; Fonnegra, R; Jiménez, S L; Osorio, R G; Saldarriaga, M; Díaz, A

    2000-11-01

    Thirty-one of 75 extracts of plants used by traditional healers for snakebites, had moderate or high neutralizing ability against the haemorrhagic effect of Bothrops atrox venom from Antioquia and Chocó, north-western Colombia. After preincubation of several doses of every extract (7.8-4000 microg/mouse) with six minimum haemorrhagic doses (10 microg) of venom, 12 of them demonstrated 100% neutralizing capacity when the mixture was i.d. injected into mice (18-20 g). These were the stem barks of Brownea rosademonte (Caesalpiniaceae) and Tabebuia rosea (Bignoniaceae); the whole plants of Pleopeltis percussa (Polypodiaceae), Trichomanes elegans (Hymenophyllaceae) and Senna dariensis (Caesalpiniaceae); rhizomes of Heliconia curtispatha (Heliconiaceae); leaves and branches of Bixa orellana (Bixaceae), Philodendron tripartitum (Araceae), Struthanthus orbicularis (Loranthaceae) and Gonzalagunia panamensis (Rubiaceae); the ripe fruits of Citrus limon (Rutaceae); leaves, branches and stem of Ficus nymphaeifolia (Moraceae). Extracts of another 19 species showed moderate neutralization (21-72%) at doses up to 4 mg/mouse, e.g. the whole plants of Aristolochia grandiflora (Aristolochiaceae), Columnea kalbreyeriana (Gesneriaceae), Sida acuta (Malvaceae), Selaginella articulata (Selaginellaceae) and Pseudoelephantopus spicatus (Asteraceae); rhizomes of Renealmia alpinia (Zingiberaceae); the stem of Strychnos xinguensis (Loganiaceae); leaves, branches and stems of Hyptis capitata (Lamiaceae), Ipomoea cairica (Convolvulaceae), Neurolaena lobata (Asteraceae), Ocimum micranthum (Lamiaceae), Piper pulchrum (Piperaceae), Siparuna thecaphora (Monimiaceae), Castilla elastica (Moraceae) and Allamanda cathartica (Apocynaceae); the macerated ripe fruits of Capsicum frutescens (Solanaceae); the unripe fruits of Crescentia cujete (Bignoniaceae); leaves and branches of Piper arboreum (Piperaceae) and Passiflora quadrangularis (Passifloraceae). When the extracts were independently administered

  20. Larvicidal potential of some plants from West Africa against Culex quinquefasciatus (Say) and Anopheles gambiae Giles (Diptera: Culicidae).

    Science.gov (United States)

    Azokou, Alain; Koné, Mamidou W; Koudou, Benjamin G; Tra Bi, Honora F

    2013-01-01

    Mosquitoes increased resistance to insecticides, and environmental concerns about the use of insecticides, pose a major challenge in the search for new molecules to deplete and incapacitate mosquito populations. Plants are the valuable source as practices consisting in exploiting plant materials as repellents, and are still in wide use throughout developing countries. The aim of the present study was to screen plants from Cτte d'Ivoire for larvicidal activity against mosquitoes. Resistant and sensitive larvae (III and IV instar) of Anopheles gambiae and Culex quinquefasciatus were exposed to crude ethanol extracts (90%) of 45 plants and viability observed after 30 min, 6, 12 and 24 h postincubation. After partition of active extracts, each fraction (hexane and chloroform washed with NaCl 1%, tannins and aqueous) was tested using the same protocol at various concentrations (1000- 31.2 ppm). Of 49 extracts tested, 7 exhibited high potential (LC50 = 80 to 370 ppm) against resistant and sensitive III and IV instar larvae of An. gambiae and Cx. quinquefasciatus. These extracts were from Cissus populnea, Cochlospermum planchonii, Heliotropium indicum, Phyllanthus amarus, Vitex grandifolia and Alchornea cordifolia. However, three most active plant species (LC50 = 80- 180 ppm) were Cs. populnea, Cm. planchonii and P. amarus Their hexane and chloroform fractions showed high larvicidal activity. This study demonstrated that plants from Cτte d'Ivoire have a real potential for malaria, yellow fever, filarial and dengue vector control. Those could be used as sources or provide lead compounds for the development of safe plant-based biocides.

  1. Microsatellite marker development by partial sequencing of the sour passion fruit genome (Passiflora edulis Sims).

    Science.gov (United States)

    Araya, Susan; Martins, Alexandre M; Junqueira, Nilton T V; Costa, Ana Maria; Faleiro, Fábio G; Ferreira, Márcio E

    2017-07-21

    The Passiflora genus comprises hundreds of wild and cultivated species of passion fruit used for food, industrial, ornamental and medicinal purposes. Efforts to develop genomic tools for genetic analysis of P. edulis, the most important commercial Passiflora species, are still incipient. In spite of many recognized applications of microsatellite markers in genetics and breeding, their availability for passion fruit research remains restricted. Microsatellite markers in P. edulis are usually limited in number, show reduced polymorphism, and are mostly based on compound or imperfect repeats. Furthermore, they are confined to only a few Passiflora species. We describe the use of NGS technology to partially assemble the P. edulis genome in order to develop hundreds of new microsatellite markers. A total of 14.11 Gbp of Illumina paired-end sequence reads were analyzed to detect simple sequence repeat sites in the sour passion fruit genome. A sample of 1300 contigs containing perfect repeat microsatellite sequences was selected for PCR primer development. Panels of di- and tri-nucleotide repeat markers were then tested in P. edulis germplasm accessions for validation. DNA polymorphism was detected in 74% of the markers (PIC = 0.16 to 0.77; number of alleles/locus = 2 to 7). A core panel of highly polymorphic markers (PIC = 0.46 to 0.77) was used to cross-amplify PCR products in 79 species of Passiflora (including P. edulis), belonging to four subgenera (Astrophea, Decaloba, Distephana and Passiflora). Approximately 71% of the marker/species combinations resulted in positive amplicons in all species tested. DNA polymorphism was detected in germplasm accessions of six closely related Passiflora species (P. edulis, P. alata, P. maliformis, P. nitida, P. quadrangularis and P. setacea) and the data used for accession discrimination and species assignment. A database of P. edulis DNA sequences obtained by NGS technology was examined to identify microsatellite repeats in

  2. Pegamento da enxertia em diferentes combinações de variedades e espécies utilizadas como copa e como porta-enxertos de maracujazeiro

    Directory of Open Access Journals (Sweden)

    Givanildo Roncatto

    2011-09-01

    Full Text Available O Brasil é o maior produtor de maracujá. Mas, apesar da posição de destaque, a vida útil do maracujazeiro vem sendo reduzida, principalmente, devido aos danos causados por doenças do sistema radicular. A enxertia com espécies nativas e resistentes a doenças apresenta-se como alternativa de produção. Com isso, objetivou-se avaliar o pegamento da enxertia nas combinações de variedades-copa e espécies de porta-enxertos de maracujazeiro. O trabalho foi desenvolvido em viveiro telado (50% de sombreamento, na Embrapa Acre, em Rio Branco-AC, entre setembro e dezembro de 2007. A variedade-copa utilizada para todos os tratamentos foi o maracujazeiro-amarelo 'FB 100' e 'FB 200' do viveiro Flora Brasil (Araguari-MG e outras 5 variedades regionais (UFAC-Universidade Federal do Acre, Rio Branco-AC, e os porta-enxertos foram Passiflora edulis Sims (maracujazeiro-amarelo (acesso Cuiabá-MT, P. alata, P. edulis (maracujazeiro-roxo e P. quadrangularis (acesso Guiratinga-MT, P. serrato-digitata (acesso IAC-Campinas-SP. As sementes foram previamente embebidas em água destilada por cerca de 24 h e posteriormente semeadas em tubetes plásticos (25x5cm com substrato Plantmax@. O método de enxertia utilizado foi o de fenda cheia no topo hipocotiledonar, tendo os porta-enxertos as seguintes características: três folhas verdadeiras e altura de plântula variável (6 a 8cm, 30 a 90 dias após a semeadura. Decapitaram-se as plântulas na altura dos cotilédones com lâmina de aço, as quais foram mergulhadas em água sanitária a 70%, a cada enxertia realizada. Os enxertos foram obtidos de plântula inteira, com cerca de 10 cm de comprimento, fazendo-se a limpeza das folhas. As combinações de melhor desempenho em relação ao pegamento da enxertia foram UFAC 07 sobre P. edulis (maracujazeiro-roxo e P. alata, UFAC 38 sobre P. edulis (maracujazeiro-amarelo, P. edulis (maracujazeiro-roxo e P. alata, UFAC 64 sobre P. serrato-digitata, com 100% de pegamento