WorldWideScience

Sample records for cinnosti sujb pri

  1. USVAJANJE JEZIKA PRI OTROCIH

    OpenAIRE

    Kurmanšek, Urška

    2010-01-01

    Namen diplomske naloge je predstaviti usvajanje jezika pri otrocih, osnovne pogoje in stopnje le-tega in razložiti Chomskyijev pogled na jezikovno prirojenost in njegovo prepričanje, da učenje jezika ni nekaj, kar bi se otrok naučil, ampak da se mu enostavno zgodi na poti v razvoju v za to primernem socialnem okolju. Temu sledi razlaga Chomskyijeve in Skinnerjeve teorije o usvajanju jezika z namenom, da se poudarijo njuna različna pogleda na ta proces. Diplomska naloga je tako osredotočena na...

  2. Remotely sensing the photochemical reflectance index, PRI

    Science.gov (United States)

    Vanderbilt, Vern; Daughtry, Craig; Dahlgren, Robert

    2015-09-01

    In remote sensing, the Photochemical Reflectance Index (PRI) provides insight into physiological processes occurring inside leaves in a plant stand. Developed by1,2, PRI evolved from laboratory reflectance measurements of individual leaves. Yet in a remotely sensed image, a pixel measurement may include light from both reflecting and transmitting leaves. We compared values of PRI based upon polarized reflectance and transmittance measurements of water and nutrient stressed leaves. Our results show the polarized leaf surface reflection should be removed when calculating PRI and that the leaf physiology information is in leaf interior reflectance, not leaf transmittance.

  3. Procesi pri transportu kohezivnih sedimentov

    OpenAIRE

    Pečovnik, Urška

    2015-01-01

    Pri procesih transporta, kot sta usedanje in privzdigovanje, obstajajo med kohezivnimi in nekohezivnimi sedimenti bistvene razlike. Obstoječi model PCFLOW3D ima v sedimentacijskem modulu vgrajene enačbe za nekohezivne sedimente, zato smo predpostavili, da na območjih kjer so sedimenti kohezivni, ne daje pravilnih rezultatov. Poiskali smo enačbe za račun usedanja, privzdigovanja in transporta kohezivnih sedimentov in jih primerjali z že vgrajenimi enačbami v modelu. Poiskali smo parametre, ki ...

  4. Linking Knowledge and Action: PRI's Community Consultant.

    Science.gov (United States)

    Spencer, Gregory P.

    Within the Partnership for Rural Improvement (PRI), community consultants operate within three complex sets of relationships: client groups, the organizational structure of PRI, and the local operational base. Community consultants are responsible for developing and facilitating rural development and for providing assistance in community and…

  5. DnaT is a PriC-binding protein.

    Science.gov (United States)

    Huang, Chien-Chih; Huang, Cheng-Yang

    2016-09-01

    DnaT and PriC are replication restart primosomal proteins required for re-initiating chromosomal DNA replication. DnaT is a component of the PriA-dependent primosome, while PriC belongs to the PriC-dependent primosome. Whether DnaT can interact with PriC is still unknown. In this study, we define a direct interaction between PriC, a key initiator protein in PriC-mediated DNA replication restart, and DnaT, a DnaB/C complex loader protein, from Klebsiella pneumoniae. In fluorescence titrations, PriC bound to single-stranded DNA with a binding-site size of approximately 9 nt. Gold nanoparticle assay showed that the solution of DnaT-PriC changed from red to purple, which indicated the protein-protein interactions due to gold nanoparticle aggregate. In addition, this DnaT-PriC complex could be co-purified by the heparin HP column. Surface plasmon resonance analysis showed that the Kd value of DnaT bound to PriC was 2.9 × 10(-8) M. These results constitute a pioneering study of the DnaT-PriC interaction and present a putative link between the two independent replication restart pathways, namely, PriA- and PriC-dependent primosome assemblies. Further research can directly focus on determining how DnaT binds to the PriC-SSB-DNA tricomplex and regulates the PriC-dependent replication restart.

  6. Presence of pRI1:

    DEFF Research Database (Denmark)

    Migura, Lourdes Garcia; Hasman, Henrik; Jensen, Lars Bogø

    2009-01-01

    This study focused on the molecular characterization of a small cryptic, mobilizable plasmid (6038 bp) sequenced from an E. faecium 9631160-1 of poultry origin. Sequence analysis of pRI1 revealed seven open reading frames. pRI1 contained an IS 100% identical to ISEfa4. This insertion element...... disrupted a putative mobilization gene (mobA) which presented 99% similarity to the one described in plasmid pJS42 (NC_010291). pRI1 harbored a cluster of four coding sequences which exhibited a homology to those described in contig 658 (from nucleotide 8940 to nt 10515) of E. faecium DO. In addition, a rep...... almost identical to the repA from the pEFNP1 and pKQ10 plasmids from E. faecium was also identified. Presence of the pRI1 replication initiation gene (rep) was analyzed in a panel of 159 E. faecium isolates of human and animal origin from different European countries, of which 60 tested positive...

  7. Remotely Sensing the Photochemical Reflectance Index (PRI)

    Science.gov (United States)

    Vanderbilt, Vern

    2015-01-01

    In remote sensing, the Photochemical Reflectance Index (PRI) provides insight into physiological processes occurring inside the leaves in a stand of plants. Developed by Gamon et al., (1990 and 1992), PRI evolved from laboratory measurements of the reflectance of individual leaves (Bilger et al.,1989). Yet in a remotely sensed image, a pixel measurement may include light from both reflecting and transmitting leaves. We conducted laboratory experiments comparing values of PRI based upon polarized reflectance and transmittance measurements of water and nutrient stressed leaves. We illuminated single detached leaves using a current controlled light source (Oriel model 66881) and measured the leaf weight using an analytical balance (Mettler model AE 260) and the light reflected and transmitted by the leaf during dry down using two Analytical Spectral Devices spectroradiometers. Polarizers on the incident and reflected light beams allowed us to divide the leaf reflectance into two parts: a polarized surface reflectance and a non-polarized 'leaf interior' reflectance. Our results underscore the importance when calculating PRI of removing the leaf surface reflection, which contains no information about physiological processes ongoing in the leaf interior. The results show that the leaf physiology information is in the leaf interior reflectance, not the leaf transmittance. Applied to a plant stand, these results suggest use of polarization measurements in sun-view directions that minimize the number of sunlit transmitting leaves in the sensor field of view.

  8. Improved method for pulse sorting based on PRI transform

    Science.gov (United States)

    Ren, Chunhui; Cao, Junqing; Fu, Yusheng; Barner, Kenneth E.

    2014-06-01

    To solve the problem of pulse sorting in complex electromagnetic environment, we propose an improved method for pulse sorting through in-depth analysis of the PRI transform algorithm principle and the advantages and disadvantages in this paper. The method is based on the traditional PRI transform algorithm, using spectral analysis of PRI transform spectrum to estimate the PRI centre value of jitter signal. Simulation results indicate that, the improved sorting method overcome the shortcomings of the traditional PRI jitter separation algorithm which cannot effectively sort jitter pulse sequence, in addition to the advantages of simple and accurate.

  9. Differential interference of vitamin D analogs PRI-1906, PRI-2191, and PRI-2205 with the renewal of human colon cancer cells refractory to treatment with 5-fluorouracil.

    Science.gov (United States)

    Kotlarz, Agnieszka; Przybyszewska, Małgorzata; Swoboda, Paweł; Miłoszewska, Joanna; Grygorowicz, Monika Anna; Kutner, Andrzej; Markowicz, Sergiusz

    2016-04-01

    This study was aimed to determine whether hypocalcemic analogs of active forms of vitamins D modulate expression of genes related to stem-like phenotype in colon cancer cell lines HT-29 and HCT-116 undergoing renewal after the treatment with 5-fluorouracil (5-FU). Both lines express vitamin D receptor, but differ in differentiation stage and vitamin D sensitivity. Cells that resisted the 5-FU exposure were treated with synthetic analog of 1,25-dihydroxyvitamin D2 (PRI-1906) and analogs of 1,25-dihydroxyvitamin D3 (PRI-2191 and PRI-2205). Proliferative activity was more profoundly affected by vitamin D analogs in HT-29/5-FU than in HCT-116/5-FU cells. In HT-29/5-FU cells, analogs PRI-1906 and PRI-2191 downregulated the expression of genes related to survival, re-growth, and invasiveness during renewal, while PRI-2205 increased expression of genes related to differentiation only. In HCT-116/5-FU cells, PRI-2191 decreased the expression of stemness- and angiogenesis-related genes, whereas PRI-1906 augmented their expression. The effects in HCT-116/5-FU cells were observed at higher concentrations of the analogs than those used for HT-29/5-FU cells. Out of the series of analogs studied, PRI-2191 might be used to counteract the renewal of both moderately and poorly differentiated cancer cells following conventional treatment.

  10. [Sensitivity study of a revised leaf photochemical reflectance index (PRI)].

    Science.gov (United States)

    Wu, Chao-yang; Niu, Zheng; Tang, Quan

    2008-09-01

    Photochemical reflectance index (PRI) defined as a normalized difference index using two narrow reflectance bands at 531 and 570 nm that are closely related to xanthophyll cycle pigment content has been successfully used to estimate leaf photosynthetic light use efficiency (LUE) across species which vary in water content and nitrogen concentration. Previous research demonstrated that a consistent relationship could be established between PRI and LUE calculated from gas exchange measurements at the leaf, small canopy, and full forest or crop canopy scales. However, a number of problems, such as the saturation of PRI when LUE exceeds 0.03 mol CO2 mol(-1) PPED (photosynthetic photon flux density) and disjunctive relationships of PRI and LUE in seasonal changes, still existed and need to be handled in order to evaluate LUE more accurately. A sensitivity study of a revised PRI with four leaf parameters was performed based on PROSPECT model in the present article to study the effects of different biochemical concentrations on leaf SR-PRI (simple ratio PRI). Sensitivity study proved that leaf SR-PRI is more sensitive to leaf mesophyll structure parameter (N) and chlorophyll a + b content (c(ab)) than parameters of dry matter content (c(m)) and equivalent water thickness (c(w)), indicating that leaf mesophyll structure parameter (N) and chlorophyll a + b content (c(ab)) should be especially considered when acquiring leaf SR-PRI. And changes in the two parameters would cause large variation in SR-PRI which would reduce the precision for estimating light use efficiency. Validation study of SR-PRI was carried out in the analysis and the results proved that SR-PRI can also be a feasible index of estimating LUE for four species of plants with correlation coefficients better than that of PRI and LUE. The advantage of SR-PRI compared to PRI is its much clearer physical meaning and its sensitivity to the changes in reflectance at 531 nm which serves as a core parameter to evaluate

  11. Synthesis and Biological Activity of Diastereomeric and Geometric Analogs of Calcipotriol, PRI-2202 and PRI-2205, Against Human HL-60 Leukemia and MCF-7 Breast Cancer Cells.

    Science.gov (United States)

    Milczarek, Magdalena; Chodyński, Michał; Filip-Psurska, Beata; Martowicz, Agnieszka; Krupa, Małgorzata; Krajewski, Krzysztof; Kutner, Andrzej; Wietrzyk, Joanna

    2013-10-31

    Diastereomeric and geometric analogs of calcipotriol, PRI-2202 and PRI-2205, were synthesized as advanced intermediates from vitamin D C-22 benzothiazoyl sulfones and side-chain aldehydes using our convergent strategy. Calcitriol, calcipotriol (PRI-2201) and tacalcitol (PRI-2191) were used as the reference compounds. Among a series of tested analogs the diastereomeric analog PRI-2202 showed the strongest antiproliferative activity on the human breast cancer cell line MCF-7, whereas the geometric analog PRI-2205 was the weakest. Both analogs were less potent in antiproliferative activity against HL-60 cells compared to the reference compounds. The ability to potentiate antiproliferative effect of cisplatin or doxorubicin against HL-60 cells or that of tamoxifen against the MCF-7 cell line was observed at higher doses of PRI-2202 or PRI-2205 than those of the reference compounds. The proapoptotic activity of tamoxifen, expressed as the diminished mitochondrial membrane potential, as well as the increased phosphatidylserine expression, was partially attenuated by calcitriol, PRI-2191, PRI-2201 and PRI-2205. The treatment of the MCF-7 cells with tamoxifen alone resulted in an increase in VDR expression. Moreover, a further increase in VDR expression was observed when the analogs PRI-2201 or PRI-2205, but not PRI-2191, were used in combination with tamoxifen. This observation could partially explain the potentiation of the antiproliferative effect of tamoxifen by vitamin D analogs.

  12. PRI: An Periodically Receiver-Initiated Task Scheduling Algorithm

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Task scheduling is a key problem for the distributed computation. This thesis analyzes receiver-initiated(RI) task scheduling algorithm, finds its weakness and presents an improved algorithm-PRI algorithm. This algorithm schedules the concurrent tasks onto network of workstation dynamically at runtime, and initiates task scheduling by the node of low load. The threshold on each node can be modified according to the system information which is periodically detected. Meanwhile, the detecting period can be adjusted in terms of the change of the system state. The result of the experiments shows that the PRI algorithm is superior to the RI algorithm.

  13. VPLIV IZVORA NADZOROVANJA NA NEKATERE DEJAVNIKE PRI DELU REVIZORJEV

    OpenAIRE

    Voje, Melita

    2012-01-01

    Izvor nadzorovanja (angl. Locus of Control) je psihološki pojem, ki ločuje posameznike na tiste s poudarjenim notranjim izvorom nadzorovanja – internaliste, in tiste s poudarjenim zunanjim izvorom nadzorovanja – eksternaliste. Razlika med njimi je v prepričanju, da kar se jim v življenju dogaja, je pri enih posledica njihovega lastnega napora, vloženega znanja, truda, pri drugih pa posledica sreče, usode. V drugem delu naloge smo opredelili pojem izvora nadzorovanja ter način ugotavljanja...

  14. Geology of Planina pri Jezeru and its environs (Slovenia

    Directory of Open Access Journals (Sweden)

    Mihael Brenčič

    2008-06-01

    Full Text Available The article describes the results of detailed geological mapping of the wider environs of Planina pri Jezeru north of Bohinj. In the literature the Upper Triassic massive and bedded limestone, Jurassic limestone, Cretaceous clastic rocks and Pleistocene sediments have been reported in this area up to now. The article supplements existing lithostratigraphic information by defining exact locations of the Pleistocene sediments at Planina pri Jezeru and Planina v Lazu, and of Jurassic rocks. In Poljane a Neptunian dyke and bioclastic limestone, while north of Mizčna glava a flat-bedded microsparitic limestone were described for the first time.Correlation to the Triglav Lakes Valley Jurassic beds indicates equivalent facieses. Allochthon Cretaceous biocalcarenite and jasper situated east of Planina pri Jezeru are also described due to their importance for further glaciological studies. The results show that the recent lake Jezero na Planini pri Jezeru is formed due to the accumulation of Pleistocene glacial-lacustrine fine-grained sediment in till,deposited on the Dachstein limestone.

  15. Canopy Level Chlorophyll Fluorescence and the PRI in a Cornfield

    Science.gov (United States)

    Middleton, Elizabeth M.; Cheng, Yen-Ben; Corp, Lawrence A.; Campbell, Petya K. E.; Huemmrich, K. Fred; Zhang, Qingyuan; Kustas, William P.

    2012-01-01

    Two bio-indicators, the Photochemical Reflectance Index (PRI) and solar-induced red and far-red Chlorophyll Fluorescence (SIF), were derived from directional hyperspectral observations and studied in a cornfield on two contrasting days in the growing season. Both red and far-red SIF exhibited higher values on the day when the canopy in the early senescent stage, but only the far-red SIF showed sensitivity to viewing geometry. Consequently, the red/far-red SIF ratio varied greatly among azimuth positions while the largest values were obtained for the "hotspot" at both growth stages. This ratio was lower (approx.0.88 +/- 0.4) in early July than in August when the ratio approached equivalence (near approx.1). In concert, the PRI exhibited stronger responses to both zenith and azimuth angles and different values on the two growth stages. The potential of using these indices to monitor photosynthetic activities needs further investigation

  16. The Primitive Material Explorer (PriME) Mission

    Science.gov (United States)

    Cochran, Anita L.; Weaver, H. A.; Science, PriME; Engineering Teams

    2010-10-01

    The Primitive Material Explorer (PriME) Mission is a proposed Discovery mission that will rendezvous with comet 46P/Wirtanen in 2021 in order to 1) clarify the roles played by comets in the formation and evolution of the Solar System and the origin of life; 2) ascertain the bulk physical properties, the surface geology, and the sources of activity in a fresh comet nucleus; and 3) investigate the compositional diversity of primitive material in the Solar System. PriME teams an experienced group of comet scientists (led by PI Anita Cochran and by DPI Harold Weaver) with university and industrial partners. The PriME payload accomplishes the mission objectives with only three instruments. MASPEX (MAss Spectrometer for Planetary EXploration) has higher mass resolution and is more sensitive than any mass spectrometer ever flown. MASPEX will measure D/H in H2O, noble gases, isotopes of many species, and complex molecular compounds to test solar nebula models and the role comets played in delivering water and other biologically important materials throughout the Solar System. The VIS (Visible Imaging System), consisting of a Narrow Angle Camera (NAC) and Wide-Angle Camera (WAC), will constrain the conditions under which the building blocks of the outer Solar System were assembled by measuring key physical properties of the nucleus of 46P/Wirtanen. Using the radio antenna and close flybys of the nucleus, PriME will determine the mass of the nucleus to an accuracy of 1% and the bulk density and average porosity of the nucleus to better than 5%. All spacecraft subsystems have significant planetary flight heritage. The spacecraft is a high-heritage derivative of the Kepler and Deep Impact spacecrafts, compatible with the three launch vehicle families specified in the Discovery Announcement of Opportunity.

  17. Prodifferentiation Activity of Novel Vitamin D₂ Analogs PRI-1916 and PRI-1917 and Their Combinations with a Plant Polyphenol in Acute Myeloid Leukemia Cells.

    Science.gov (United States)

    Nachliely, Matan; Sharony, Ehud; Bolla, Narasimha Rao; Kutner, Andrzej; Danilenko, Michael

    2016-07-05

    1α,25-dihydroxyvitamin D₃ (1,25D3) is a powerful differentiation inducer for acute myeloid leukemia (AML) cells. However, 1,25D3 doses required for differentiation of AML cells may cause lethal hypercalcemia in vivo. There is evidence that vitamin D₂ is less toxic than vitamin D₃ in animals. Here, we determined the differentiation effects of novel analogs of 1α,25-dihydroxyvitamin D₂ (1,25D2), PRI-1916 and PRI-1917, in which the extended side chains of their previously reported precursors (PRI-1906 and PRI-1907, respectively) underwent further 24Z (24-cis) modification. Using four human AML cell lines representing different stages of myeloid maturation (KG-1a, HL60, U937, and MOLM-13), we found that the potency of PRI-1916 was slightly higher or equal to that of PRI-1906 while PRI-1917 was significantly less potent than PRI-1907. We also demonstrated that 1,25D2 was a less effective differentiation agent than 1,25D3 in these cell lines. Irrespective of their differentiation potency, all the vitamin D₂ derivatives tested were less potent than 1,25D3 in transactivating the DR3-type vitamin D response elements. However, similar to 1,25D3, both 1,25D2 and its analogs could strongly cooperate with the plant polyphenol carnosic acid in inducing cell differentiation and inhibition of G1-S cell cycle transition. These results indicate that the 24Z modification has contrasting effects on the differentiation ability of PRI-1906 and PRI-1907 and that the addition of a plant polyphenol could result in a similar extent of cell differentiation induced by different vitamin D compounds. The enhanced antileukemic effects of the tested combinations may constitute the basis for the development of novel approaches for differentiation therapy of AML.

  18. Prodifferentiation Activity of Novel Vitamin D2 Analogs PRI-1916 and PRI-1917 and Their Combinations with a Plant Polyphenol in Acute Myeloid Leukemia Cells

    Directory of Open Access Journals (Sweden)

    Matan Nachliely

    2016-07-01

    Full Text Available 1α,25-dihydroxyvitamin D3 (1,25D3 is a powerful differentiation inducer for acute myeloid leukemia (AML cells. However, 1,25D3 doses required for differentiation of AML cells may cause lethal hypercalcemia in vivo. There is evidence that vitamin D2 is less toxic than vitamin D3 in animals. Here, we determined the differentiation effects of novel analogs of 1α,25-dihydroxyvitamin D2 (1,25D2, PRI-1916 and PRI-1917, in which the extended side chains of their previously reported precursors (PRI-1906 and PRI-1907, respectively underwent further 24Z (24-cis modification. Using four human AML cell lines representing different stages of myeloid maturation (KG-1a, HL60, U937, and MOLM-13, we found that the potency of PRI-1916 was slightly higher or equal to that of PRI-1906 while PRI-1917 was significantly less potent than PRI-1907. We also demonstrated that 1,25D2 was a less effective differentiation agent than 1,25D3 in these cell lines. Irrespective of their differentiation potency, all the vitamin D2 derivatives tested were less potent than 1,25D3 in transactivating the DR3-type vitamin D response elements. However, similar to 1,25D3, both 1,25D2 and its analogs could strongly cooperate with the plant polyphenol carnosic acid in inducing cell differentiation and inhibition of G1–S cell cycle transition. These results indicate that the 24Z modification has contrasting effects on the differentiation ability of PRI-1906 and PRI-1907 and that the addition of a plant polyphenol could result in a similar extent of cell differentiation induced by different vitamin D compounds. The enhanced antileukemic effects of the tested combinations may constitute the basis for the development of novel approaches for differentiation therapy of AML.

  19. Relationships between the photochemical reflectance index (PRI) and chlorophyll fluorescence parameters and plant pigment indices at different leaf growth stages.

    Science.gov (United States)

    Rahimzadeh-Bajgiran, Parinaz; Munehiro, Masashi; Omasa, Kenji

    2012-09-01

    This study aimed to evaluate the photochemical reflectance index (PRI) for assessing plant photosynthetic performance throughout the plant life cycle. The relationships between PRI, chlorophyll fluorescence parameters, and leaf pigment indices in Solanum melongena L. (aubergine; eggplant) were studied using photosynthetic induction curves both in short-term (diurnal) and long-term (seasonal) periods under different light intensities. We found good correlations between PRI/non-photochemical quenching (NPQ) and PRI/electron transport rate (ETR) in the short term at the same site of a single leaf but these relationships did not hold throughout the life of the plant. In general, changes in PRI owing to NPQ or ETR variations in the short term were PRI was highly correlated to plant pigments, especially chlorophyll indices measured by spectral reflectance. Moreover, relationships of steady-state PRI/ETR and steady-state PRI/photochemical yield of photosystem II (Φ(PSII)) measured at uniform light intensity at different life stages proved that overall photosynthesis capacity and steady-state PRI were better correlated through chlorophyll content than NPQ and xanthophylls. The calibrated PRI index accommodated these pigments effects and gave better correlation with NPQ and ETR than PRI. Further studies of PRI indices based on pigments other than xanthophylls, and studies on PRI mechanisms in different species are recommended.

  20. Analiza toplotnih izgub pri različnih okenskih sistemih

    OpenAIRE

    Movrin, Miran

    2014-01-01

    Ob vseobsegajočih razvojnih trendih tudi okna niso bila izvzeta, njihov razvoj je med drugim šel tudi v smeri zmanjševanja toplotnih izgub. Raziskovali smo, kolikšne so toplotne izgube pri okenskih sistemih z različno okvirno konstrukcijo, s steklom in z distančno letvijo. Ugotovili smo, da imajo lesena, aluminijasta in PVC-okna z novimi PVC-distančniki manjše toplotne izgube od dosedanjih izdelanih s klasičnimi, aluminijastimi distančniki. Ugotovitev pa je tudi razultat primerjave velikosti ...

  1. Pomen čustvene inteligentnosti pri vodenju v zdravstveni negi

    OpenAIRE

    Meško, Janja

    2011-01-01

    Diplomsko delo z naslovom pomen čustvene inteligentnosti pri vodenju v zdravstveni negi je teoretično. S pomočjo deskriptivne metode dela in študija različne literature smo predstavili pomen čustvene inteligentnosti, vodenja in predvsem povezavo obeh pojmov v zdravstveni negi. Dandanes je čustvena inteligentnost vedno bolj v ospredju ter vedno bolj središče zanimanja, saj so časi, v katerem so bila čustva označena kot nekaj, kar za posel ni pomembno, minili. Pregledali smo domačo in tujo lite...

  2. VEDENJE SLOVENSKIH POTROŠNIKOV PRI SPLETNEM NAKUPOVANJU

    OpenAIRE

    Kos, Jasmina

    2015-01-01

    Težko bi rekli, da bo spletno nakupovanje izpodrinilo klasično nakupovanje v fizičnih trgovinah in trgovskih centrih, vendar pa kljub temu lahko rečemo, da pričakujemo v naslednjih letih na spletu še več trgovin in povečano rast spletnih potrošnikov. Spletne trgovine imajo namreč mnogo prednosti, saj lahko udobno iz naslonjača kadarkoli in kjerkoli (dokler imamo internetno povezavo) kupimo izdelek, ki ga želimo, brez vsiljevanja prodajalčevega mnenja, čakanja na blagajni, iskanja parkirnega p...

  3. POMEN VAROVANJA INTELEKTUALNE LASTNINE PRI SNOVANJU POLITIKE PODJETJA

    OpenAIRE

    Budja, Matej

    2014-01-01

    V diplomskem seminarju sem ugotavljal medsebojno soodvisnost politike podjetja in pomena varovanja intelektualne lastnine. Osredotočil sem se na inovativno slovensko podjetje Eurel d.o.o., katerega vodi Štefan Kuzma, teoretični in praktični strokovnjak tako pri vodenju podjetja, kot tudi v zvezi z varovanjem IL. Ugotovil sem sledeče: • Eurel proizvaja izdelke skladno z zahtevanimi specifikacijami naročnikov in v dogovorjenih rokih • Zadovoljnost odjemalcev je njihova prioriteta • ...

  4. Three causes of variation in the photochemical reflectance index (PRI) in evergreen conifers.

    Science.gov (United States)

    Wong, Christopher Y S; Gamon, John A

    2015-04-01

    The photochemical reflectance index (PRI) reflects diurnal xanthophyll cycle activity and is also influenced by seasonally changing carotenoid : Chl pigment ratios. Both changing pigment pools and xanthophyll cycle activity contribute to photoprotection in evergreen conifers exposed to boreal winters, but they operate over different timescales, and their relative contribution to the PRI signal has often been unclear. To clarify these responses and their contribution to the PRI signal, leaf PRI, pigment composition, temperature and irradiance were monitored over 2 yr for two evergreen conifers (Pinus contorta and Pinus ponderosa) in a boreal climate. PRI was affected by three distinct processes operating over different timescales and exhibiting contrasting spectral responses. Over the 2 yr study period, the greatest change in PRI resulted from seasonally changing carotenoid : Chl pigment ratios, followed by a previously unreported shifting leaf albedo during periods of deep cold. Remarkably, the smallest change was attributable to the xanthophyll cycle. To properly distinguish these three effects, interpretation of PRI must consider temporal context, physiological responses to evolving environmental conditions, and spectral response. Consideration of the separate mechanisms affecting PRI over different timescales could greatly improve efforts to monitor changing photosynthetic activity using optical remote sensing.

  5. Draft genome sequence of the antagonistic rhizosphere bacterium Serratia plymuthica strain PRI-2C.

    Science.gov (United States)

    Garbeva, P; van Elsas, J D; de Boer, W

    2012-08-01

    Serratia plymuthica strain PRI-2C is a rhizosphere bacterial strain with antagonistic activity against different plant pathogens. Here we present the 5.39-Mb (G+C content, 55.67%) draft genome sequence of S. plymuthica strain PRI-2C with the aim of providing insight into the genomic basis of its antagonistic activity.

  6. PRI Play Important Role in West-East Project · Interview with PIR Director Xue Zhenkui

    Institute of Scientific and Technical Information of China (English)

    Xiao Lu

    2002-01-01

    @@ Pipeline Research Institute of CNPC (PRI) is playing a more and more important role in the oil and natural gas pipeline industrial development in the present-day China. The pipeline technology and equipment developed by PRI have proven costeffective and extremely useful in the country's WestEast gas transmission pipeline project

  7. Partnership for Rural Improvement: PRI in Transition. Annual Report 1979-80.

    Science.gov (United States)

    Hagood, Richard A.; And Others

    Covering 1979-80, which was the final year of the initial four year grant period for the Partnership for Rural Improvement (PRI), this report details the major assessments and program adjustments made during that year. Several program developments of critical importance to understanding the transition occurring in PRI are highlighted in the…

  8. Diffuse sky radiation influences the relationship between canopy PRI and shadow fraction

    Science.gov (United States)

    Mõttus, Matti; Takala, Tuure L. H.; Stenberg, Pauline; Knyazikhin, Yuri; Yang, Bin; Nilson, Tiit

    2015-07-01

    The Photochemical Reflectance Index (PRI) of green leaves is an indicator of photosynthetic downregulation: when the photosynthetic apparatus is close to the saturation limit, PRI becomes dependent on light conditions. Therefore, by measuring the PRI of leaves under different local irradiance conditions, it should be possible to determine the saturation level of the leaves and obtain information on the light use efficiency (LUE) of a vegetation canopy. The dependence of PRI on the ratio of sunlit to shaded foliage (quantified by the canopy shadow fraction) in the field of view of an instrument has been used to remotely measure canopy LUE on clear days. However, besides photosynthetic downregulation, the dependence of canopy PRI on shadow fraction is affected by the blue sky radiation caused by scattering in the atmosphere. To quantify this effect on remotely sensed PRI, we present the underlying definitions relating leaf and canopy PRI and perform the required calculations for typical midsummer conditions in Central Finland. We demonstrate that the effect of blue sky radiation on the variation of PRI with canopy shadow fraction is similar in shape and magnitude to that of LUE variations reported in literature.

  9. Evidence for a cytoplasmic microprocessor of pri-miRNAs.

    Science.gov (United States)

    Shapiro, Jillian S; Langlois, Ryan A; Pham, Alissa M; Tenoever, Benjamin R

    2012-07-01

    microRNAs (miRNAs) represent a class of noncoding RNAs that fine-tune gene expression through post-transcriptional silencing. While miRNA biogenesis occurs in a stepwise fashion, initiated by the nuclear microprocessor, rare noncanonical miRNAs have also been identified. Here we characterize the molecular components and unique attributes associated with the processing of virus-derived cytoplasmic primary miRNAs (c-pri-miRNAs). RNA in situ hybridization and inhibition of cellular division demonstrated a complete lack of nuclear involvement in c-pri-miRNA cleavage while genetic studies revealed that maturation still relied on the canonical nuclear RNase III enzyme, Drosha. The involvement of Drosha was mediated by a dramatic relocalization to the cytoplasm following virus infection. Deep sequencing analyses revealed that the cytoplasmic localization of Drosha does not impact the endogenous miRNA landscape during infection, despite allowing for robust synthesis of virus-derived miRNAs in the cytoplasm. Taken together, this research describes a unique function for Drosha in the processing of highly structured cytoplasmic RNAs in the context of virus infection.

  10. PriC-mediated DNA replication restart requires PriC complex formation with the single-stranded DNA-binding protein.

    Science.gov (United States)

    Wessel, Sarah R; Marceau, Aimee H; Massoni, Shawn C; Zhou, Ruobo; Ha, Taekjip; Sandler, Steven J; Keck, James L

    2013-06-14

    Frequent collisions between cellular DNA replication complexes (replisomes) and obstacles such as damaged DNA or frozen protein complexes make DNA replication fork progression surprisingly sporadic. These collisions can lead to the ejection of replisomes prior to completion of replication, which, if left unrepaired, results in bacterial cell death. As such, bacteria have evolved DNA replication restart mechanisms that function to reload replisomes onto abandoned DNA replication forks. Here, we define a direct interaction between PriC, a key Escherichia coli DNA replication restart protein, and the single-stranded DNA-binding protein (SSB), a protein that is ubiquitously associated with DNA replication forks. PriC/SSB complex formation requires evolutionarily conserved residues from both proteins, including a pair of Arg residues from PriC and the C terminus of SSB. In vitro, disruption of the PriC/SSB interface by sequence changes in either protein blocks the first step of DNA replication restart, reloading of the replicative DnaB helicase onto an abandoned replication fork. Consistent with the critical role of PriC/SSB complex formation in DNA replication restart, PriC variants that cannot bind SSB are non-functional in vivo. Single-molecule experiments demonstrate that PriC binding to SSB alters SSB/DNA complexes, exposing single-stranded DNA and creating a platform for other proteins to bind. These data lead to a model in which PriC interaction with SSB remodels SSB/DNA structures at abandoned DNA replication forks to create a DNA structure that is competent for DnaB loading.

  11. New insights into pri-miRNA processing and accumulation in plants.

    Science.gov (United States)

    Zhang, Shuxin; Liu, Yuhui; Yu, Bin

    2015-01-01

    MicroRNAs (miRNAs) regulate many biological processes such as development, metabolism, and others. They are processed from their primary transcripts called primary miRNA transcripts (pri-miRNAs) by the processor complex containing the RNAse III enzyme, DICER-LIKE1 (DCL1), in plants. Consequently, miRNA biogenesis is controlled through altering pri-miRNA accumulation and processing, which is crucial for plant development and adaptation to environmental changes. Plant pri-miRNAs are transcribed by DNA-dependent RNA polymerase II (Pol II) and their levels are determined through transcription and degradation, whereas pri-miRNA processing is affected by its structure, splicing, alternative splicing, loading to the processor and the processor activity, which involve in many accessory proteins. Here, we summarize recent progresses related to pri-miRNA transcription, stability, and processing in plants.

  12. Correcting the relationship between PRI and shadow fraction for the blue sky effect

    Science.gov (United States)

    Mõttus, Matti

    2016-04-01

    The Photochemical Reflectance Index (PRI) is defined as the normalized difference ratio of leaf reflectance at two specific wavelengths in the green spectral region. Its value depends on the status of leaf carotenoid content, and especially that of the xanthophyll cycle pigments. Due to the dependence on the xanthophyll cycle, when the photosynthetic apparatus of green leaves is close to the saturation limit, their PRI becomes dependent on light conditions. Therefore, by measuring the PRI of leaves in the same canopy under different local irradiance conditions on a sunny day, it should be possible to determine the saturation level of the leaves. In turn, this gives information on the light use efficiency (LUE) of the vegetation canopy. The average light conditions of visible foliage elements are often quantified with the shadow fraction -- the fraction of visible foliage not lit by direct sunlight. The dependence of PRI on the shadow fraction has been used to remotely measure canopy LUE on clear days. Variations in shadow fraction have been achieved with multiangular measurement. However, besides photosynthetic downregulation, the dependence of canopy PRI on shadow fraction is affected by the blue sky radiation caused by scattering in the atmosphere. To quantify this effect on remotely sensed PRI, we present the underlying definitions relating leaf and canopy PRI and perform the required calculations for typical midsummer conditions in Central Finland. We demonstrate that the effect of blue sky radiation on the variation of PRI with canopy shadow fraction is similar in shape and magnitude to that of LUE variations reported in literature. Next, we propose a new method to assess these PRI variations in structured vegetation. We investiagate this blue sky effect on the PRI -- shadow fraction relationship with high spatial (60 cm) and spectral (9.8 nm) resolution airborne imaging spectroscopy data from Hyytiälä, Finland. We evaluate the spectral irradiance in

  13. Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing.

    Science.gov (United States)

    Auyeung, Vincent C; Ulitsky, Igor; McGeary, Sean E; Bartel, David P

    2013-02-14

    To use microRNAs to downregulate mRNA targets, cells must first process these ~22 nt RNAs from primary transcripts (pri-miRNAs). These transcripts form RNA hairpins important for processing, but additional determinants must distinguish pri-miRNAs from the many other hairpin-containing transcripts expressed in each cell. Illustrating the complexity of this recognition, we show that most Caenorhabditis elegans pri-miRNAs lack determinants required for processing in human cells. To find these determinants, we generated many variants of four human pri-miRNAs, sequenced millions that retained function, and compared them with the starting variants. Our results confirmed the importance of pairing in the stem and revealed three primary-sequence determinants, including an SRp20-binding motif (CNNC) found downstream of most pri-miRNA hairpins in bilaterian animals, but not in nematodes. Adding this and other determinants to C. elegans pri-miRNAs imparted efficient processing in human cells, thereby confirming the importance of primary-sequence determinants for distinguishing pri-miRNAs from other hairpin-containing transcripts.

  14. Monitoring seasonal and diurnal changes in photosynthetic pigments with automated PRI and NDVI sensors

    Science.gov (United States)

    Gamon, J. A.; Kovalchuck, O.; Wong, C. Y. S.; Harris, A.; Garrity, S. R.

    2015-07-01

    The vegetation indices normalized difference vegetation index (NDVI) and photochemical reflectance index (PRI) provide indicators of pigmentation and photosynthetic activity that can be used to model photosynthesis from remote sensing with the light-use-efficiency model. To help develop and validate this approach, reliable proximal NDVI and PRI sensors have been needed. We tested new NDVI and PRI sensors, "spectral reflectance sensors" (SRS sensors; recently developed by Decagon Devices, during spring activation of photosynthetic activity in evergreen and deciduous stands. We also evaluated two methods of sensor cross-calibration - one that considered sky conditions (cloud cover) at midday only, and another that also considered diurnal sun angle effects. Cross-calibration clearly affected sensor agreement with independent measurements, with the best method dependent upon the study aim and time frame (seasonal vs. diurnal). The seasonal patterns of NDVI and PRI differed for evergreen and deciduous species, demonstrating the complementary nature of these two indices. Over the spring season, PRI was most strongly influenced by changing chlorophyll : carotenoid pool sizes, while over the diurnal timescale, PRI was most affected by the xanthophyll cycle epoxidation state. This finding demonstrates that the SRS PRI sensors can resolve different processes affecting PRI over different timescales. The advent of small, inexpensive, automated PRI and NDVI sensors offers new ways to explore environmental and physiological constraints on photosynthesis, and may be particularly well suited for use at flux tower sites. Wider application of automated sensors could lead to improved integration of flux and remote sensing approaches for studying photosynthetic carbon uptake, and could help define the concept of contrasting vegetation optical types.

  15. RAČUNOVODSKE INFORMACIJE PRI UPRAVLJANJU ČLOVEŠKIH VIROV V PODJETJU X

    OpenAIRE

    2012-01-01

    V diplomski nalogi je predstavljeno upravljanje človeških virov in računovodske informacije o le teh. Opredelili smo informacije nasploh in računovodske informacije, ki jih potrebujemo pri odločitvah o človeških virih. Od vsakega podjetja je odvisno ali bo pri različnih nalogah upravljanja človeških virov uporabil računovodske informacije. Pri vseh nalogah upravljanja človeških virov so uporabne računovodske informacije, odvisno od velikosti in razširjenosti upravljanja človeških virov v...

  16. Prying Force Calculation and Design Method for T-shaped Tensile Connector with High Strength Bolt

    Institute of Scientific and Technical Information of China (English)

    Zhaoxin Hou; Guohong Huang; Chao Gong

    2015-01-01

    In order to establish the design method for T⁃shaped tensile connector with high strength bolt, the theoretical analysis is carried out. Firstly, it analyzes the performance of the connector and establishes prying force calculation model. Based on the model, prying force equation and function between bolt prying force and flange thickness is derived, and the min and max thickness requirement of flange plate under a certain tension load is then obtained. Finally, two simplified design methods of the connector are proposed, which are bolt pulling capacity method and flange plate bending capacity method.

  17. Odzivi učencev na didaktične igre pri obravnavi gibanja pri predmetu Spoznavanje okolja

    OpenAIRE

    Novak, Ines

    2016-01-01

    Igra pri pouku je priporočljiva dejavnost predvsem z vidika učencev, saj spodbuja zanimanje in vse vrste motivacije. Učenci skozi igro projicirajo svoje primanjkljaje in jih na ta način lažje premagujejo. Ni dovolj, da se učenci v šoli zgolj učijo, ampak naj bi se tam tudi dobro počutili, kar pripomore k boljšemu spremljanju pouka in trajnejšemu znanju. V teoretičnem delu magistrskega dela predstavimo kognitivni, čustveni in socialni razvoj otroka, razvoj otroške igre ter vlogo didaktične igr...

  18. Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis.

    Science.gov (United States)

    Köster, Tino; Meyer, Katja; Weinholdt, Claus; Smith, Lisa M; Lummer, Martina; Speth, Corinna; Grosse, Ivo; Weigel, Detlef; Staiger, Dorothee

    2014-09-01

    The hnRNP-like glycine-rich RNA-binding protein AtGRP7 regulates pre-mRNA splicing in Arabidopsis. Here we used small RNA-seq to show that AtGRP7 also affects the miRNA inventory. AtGRP7 overexpression caused a significant reduction in the level of 30 miRNAs and an increase for 14 miRNAs with a minimum log2 fold change of ± 0.5. Overaccumulation of several pri-miRNAs including pri-miR398b, pri-miR398c, pri-miR172b, pri-miR159a and pri-miR390 at the expense of the mature miRNAs suggested that AtGRP7 affects pri-miRNA processing. Indeed, RNA immunoprecipitation revealed that AtGRP7 interacts with these pri-miRNAs in vivo. Mutation of an arginine in the RNA recognition motif abrogated in vivo binding and the effect on miRNA and pri-miRNA levels, indicating that AtGRP7 inhibits processing of these pri-miRNAs by direct binding. In contrast, pri-miRNAs of selected miRNAs that were elevated or not changed in response to high AtGRP7 levels were not bound in vivo. Reduced accumulation of miR390, an initiator of trans-acting small interfering RNA (ta-siRNA) formation, also led to lower TAS3 ta-siRNA levels and increased mRNA expression of the target AUXIN RESPONSE FACTOR4. Furthermore, AtGRP7 affected splicing of pri-miR172b and pri-miR162a. Thus, AtGRP7 is an hnRNP-like protein with a role in processing of pri-miRNAs in addition to its role in pre-mRNA splicing.

  19. Microprocessor dynamics shows co- and post-transcriptional processing of pri-miRNAs.

    Science.gov (United States)

    Louloupi, Annita; Ntini, Evgenia; Liz, Julia; Ørom, Ulf Andersson

    2017-03-01

    miRNAs are small regulatory RNAs involved in the regulation of translation of target transcripts. miRNA biogenesis is a multi-step process starting with the cleavage of the primary miRNA transcript in the nucleus by the Microprocessor complex. Endogenous processing of pri-miRNAs is challenging to study and the in vivo kinetics of this process is not known. Here, we present a method for determining the processing kinetics of pri-miRNAs within intact cells over time using a pulse-chase approach to label transcribed RNA during 15 minutes and follow the processing within a 1-hour window after labeling with bromouridine. We show, that pri-miRNAs exhibit different processing kinetics ranging from fast over intermediate to slow processing and provide evidence that pri-miRNA processing can occur both co-transcriptionally and post-transcriptionally.

  20. Pri peptides are mediators of ecdysone for the temporal control of development.

    Science.gov (United States)

    Chanut-Delalande, Hélène; Hashimoto, Yoshiko; Pelissier-Monier, Anne; Spokony, Rebecca; Dib, Azza; Kondo, Takefumi; Bohère, Jérôme; Niimi, Kaori; Latapie, Yvan; Inagaki, Sachi; Dubois, Laurence; Valenti, Philippe; Polesello, Cédric; Kobayashi, Satoru; Moussian, Bernard; White, Kevin P; Plaza, Serge; Kageyama, Yuji; Payre, François

    2014-11-01

    Animal development fundamentally relies on the precise control, in space and time, of genome expression. Whereas we have a wealth of information about spatial patterning, the mechanisms underlying temporal control remain poorly understood. Here we show that Pri peptides, encoded by small open reading frames, are direct mediators of the steroid hormone ecdysone for the timing of developmental programs in Drosophila. We identify a previously uncharacterized enzyme of ecdysone biosynthesis, GstE14, and find that ecdysone triggers pri expression to define the onset of epidermal trichome development, through post-translational control of the Shavenbaby transcription factor. We show that manipulating pri expression is sufficient to either put on hold or induce premature differentiation of trichomes. Furthermore, we find that ecdysone-dependent regulation of pri is not restricted to epidermis and occurs over various tissues and times. Together, these findings provide a molecular framework to explain how systemic hormonal control coordinates specific programs of differentiation with developmental timing.

  1. Pri sORF peptides induce selective proteasome-mediated protein processing.

    Science.gov (United States)

    Zanet, J; Benrabah, E; Li, T; Pélissier-Monier, A; Chanut-Delalande, H; Ronsin, B; Bellen, H J; Payre, F; Plaza, S

    2015-09-18

    A wide variety of RNAs encode small open-reading-frame (smORF/sORF) peptides, but their functions are largely unknown. Here, we show that Drosophila polished-rice (pri) sORF peptides trigger proteasome-mediated protein processing, converting the Shavenbaby (Svb) transcription repressor into a shorter activator. A genome-wide RNA interference screen identifies an E2-E3 ubiquitin-conjugating complex, UbcD6-Ubr3, which targets Svb to the proteasome in a pri-dependent manner. Upon interaction with Ubr3, Pri peptides promote the binding of Ubr3 to Svb. Ubr3 can then ubiquitinate the Svb N terminus, which is degraded by the proteasome. The C-terminal domains protect Svb from complete degradation and ensure appropriate processing. Our data show that Pri peptides control selectivity of Ubr3 binding, which suggests that the family of sORF peptides may contain an extended repertoire of protein regulators.

  2. Effect of nitrogen stress on relationship of PRI and LUE during winter wheat growth period

    Science.gov (United States)

    Guo, Jianmao; Gao, Yanghua; Wang, Qian; Fei, Dunyue; Liu, Junwei

    2014-11-01

    Light use efficiency (LUE ) is an important parameter for GPP and and NPP estimation model, cause by the existing model method to estimate the actual LUE is always simple and rough, which may lead to serious bias by GPP and NPP. The photochemical reflectance index ( PRI ) has great potential for direct estimation the actual LUE. In this paper, wheat in different nitrogen treatments was designed in field trial during Wheat growing period, for obtain photosynthesis and reflective hyperspectral data, and then LUE and PRI was calculated in critical period of wheat growth. The results show that, at different growth stages under three different nitrogen conditions, LUE and PRI value were significantly increased with increasing nitrogen absorption; Last longer, more capable of absorbing nitrogen amount, the correlation between LUE and PRI was better - for example, the correlation coefficient is obviously larger in heading stage than elongation stage for same nitrogen treatment.

  3. Remote sensing of ecosystem light use efficiency with MODIS-based PRI

    Science.gov (United States)

    Goerner, A.; Reichstein, M.; Tomelleri, E.; Hanan, N.; Rambal, S.; Papale, D.; Dragoni, D.; Schmullius, C.

    2011-01-01

    Several studies sustained the possibility that a photochemical reflectance index (PRI) directly obtained from satellite data can be used as a proxy for ecosystem light use efficiency (LUE) in diagnostic models of gross primary productivity. This modelling approach would avoid the complications that are involved in using meteorological data as constraints for a fixed maximum LUE. However, no unifying model predicting LUE across climate zones and time based on MODIS PRI has been published to date. In this study, we evaluate the effectiveness with which MODIS-based PRI can be used to estimate ecosystem light use efficiency at study sites of different plant functional types and vegetation densities. Our objective is to examine if known limitations such as dependence on viewing and illumination geometry can be overcome and a single PRI-based model of LUE (i.e. based on the same reference band) can be applied under a wide range of conditions. Furthermore, we were interested in the effect of using different faPAR (fraction of absorbed photosynthetically active radiation) products on the in-situ LUE used as ground truth and thus on the whole evaluation exercise. We found that estimating LUE at site-level based on PRI reduces uncertainty compared to the approaches relying on a maximum LUE reduced by minimum temperature and vapour pressure deficit. Despite the advantages of using PRI to estimate LUE at site-level, we could not establish an universally applicable light use efficiency model based on MODIS PRI. Models that were optimised for a pool of data from several sites did not perform well.

  4. Structural and functional characterization of the CAP domain of pathogen-related yeast 1 (Pry1) protein

    Science.gov (United States)

    Darwiche, Rabih; Kelleher, Alan; Hudspeth, Elissa M.; Schneiter, Roger; Asojo, Oluwatoyin A.

    2016-06-01

    The production, crystal structure, and functional characterization of the C-terminal cysteine-rich secretory protein/antigen 5/pathogenesis related-1 (CAP) domain of pathogen-related yeast protein-1 (Pry1) from Saccharomyces cerevisiae is presented. The CAP domain of Pry1 (Pry1CAP) is functional in vivo as its expression restores cholesterol export to yeast mutants lacking endogenous Pry1 and Pry2. Recombinant Pry1CAP forms dimers in solution, is sufficient for in vitro cholesterol binding, and has comparable binding properties as full-length Pry1. Two crystal structures of Pry1CAP are reported, one with Mg2+ coordinated to the conserved CAP tetrad (His208, Glu215, Glu233 and His250) in spacegroup I41 and the other without divalent cations in spacegroup P6122. The latter structure contains four 1,4-dioxane molecules from the crystallization solution, one of which sits in the cholesterol binding site. Both structures reveal that the divalent cation and cholesterol binding sites are connected upon dimerization, providing a structural basis for the observed Mg2+-dependent sterol binding by Pry1.

  5. 新体制雷达信号 PRI 样本子图周期搜索提取方法%A New System Radar Signal Extraction Method by Searching PRI Pulse Sequence Subpattern Cycle

    Institute of Scientific and Technical Information of China (English)

    孟祥豪; 罗景青; 马贤同

    2015-01-01

    alming at the problem of radar signal extraction of interleaved pulse sequences intercepted by reconnalssance receiver,according to the description technique of pulse recurrence interval(PRI),a pulse extraction algorithm is proposed based on the searching of PRI pulse sequence subpattern(PSS)cycle.In this algorithm,the idea of searching adjacent pul-ses intervals in traditional PRI searching algorithm is replaced by that of searching the PRI-PSS cycle.A two-dimensional extraction function consisting of time of arrival and paracycle of PRI PSS is constructed to store the number of matching pul-ses which conform to a certaln assumed PRI-PSS cycle during the searching process.Then the PRI-PSS cycle is determined according to the maximum value of the function,thus the equivalent pulses can be extracted,realizing the signal extraction of complex type of PRI radar signals.The validity of this method has been verified by simulation experiments.%针对侦察接收机截获的交错脉冲序列中雷达信号的提取问题,在脉冲重复周期(PRI)样本子图描述技术的基础上,提出一种基于 PRI 样本子图周期搜索的脉冲提取算法。算法改传统 PRI 搜索法中的相邻脉冲间隔搜索思想为 PRI 样本子图周期搜索思想,通过构造由脉冲到达时间和准 PRI 样本子图周期构成的二维提取函数,将搜索过程中符合某一假定 PRI 样本子图周期的匹配脉冲数存储于二维提取函数中,然后根据二维提取函数的最大值确定 PRI 样本子图周期,提取对应的脉冲,实现复杂 PRI 类型的雷达信号提取。仿真结果验证了该方法的有效性。

  6. Diurnal and Directional Responses of Chlorophyll Fluorescence and the PRI in a Cornfield

    Science.gov (United States)

    Middleton, Elizabeth; Cheng, Y. B.; Corp, L.; Campbell, P.; Kustas, W.

    2010-01-01

    Determining the health and vigor of vegetation using high spectral resolution remote sensing is an important goal which has application to monitoring agriculture and ecosystem productivity and carbon exchange. Two spectral indices used to assess whether vegetation is performing near-optimally or exhibiting symptoms of environmental stress (e.g., drought or nutrient deficiency, non-optimal temperatures, etc.) are the Photochemical Reflectance Index (PRI) and solar-induced red and far-red Chlorophyll Fluorescence (Fs). Both the PRI and Fs capture the dynamics of photoprotection mechanisms within green foliage: the PRI is based on the association of the reflected radiation in the green spectrum with the xanthophyll cycle, whereas Fs measures the emitted radiation in the red and far-red spectrum. Fs was determined from retrievals in the atmospheric oxygen absorption features centered at 688 and 760 nm using a modified Fraunhofer Line Depth (FLD) method. We previously demonstrated diurnal and seasonal PRI differences for sunlit vs. shaded foliage in a conifer forest canopy, as expressed in the hotspot and darkspot of the Bidirectional Reflectance Function (BRF). In a USDA-ARS experimental field site located in Beltsville, MD, USA, measurements were acquired over a corn crop from a nadir view in 2008 with an ASD FieldSpec Pro (Analytical Spectral Devices, Inc., Boulder, CO, USA) to study the behavior of the PRI for sunlit and shaded foliage as captured in reflectance variations associated with the BRF, in a I m tall canopy in the vegetative growth stage. Those observations were compared to simulations obtained from two radiative transfer models. Measurements were then acquired to examine whether the PRI and Fs were influenced by view zenith and azimuth geometries at different times of day. Those measurements were made in 2010 with the Ocean Optics USB4000 Miniature Fiber Optic Spectrometer (Ocean Optics Inc., Dunedin, Florida, USA) at several times during the day on

  7. Gene structures and processing of Arabidopsis thaliana HYL1-dependent pri-miRNAs.

    Science.gov (United States)

    Szarzynska, Bogna; Sobkowiak, Lukasz; Pant, Bikram Datt; Balazadeh, Salma; Scheible, Wolf-Rüdiger; Mueller-Roeber, Bernd; Jarmolowski, Artur; Szweykowska-Kulinska, Zofia

    2009-05-01

    Arabidopsis thaliana HYL1 is a nuclear double-stranded RNA-binding protein involved in the maturation of pri-miRNAs. A quantitative real-time PCR platform for parallel quantification of 176 pri-miRNAs was used to reveal strong accumulation of 57 miRNA precursors in the hyl1 mutant that completely lacks HYL1 protein. This approach enabled us for the first time to pinpoint particular members of MIRNA family genes that require HYL1 activity for efficient maturation of their precursors. Moreover, the accumulation of miRNA precursors in the hyl1 mutant gave us the opportunity to carry out 3' and 5' RACE experiments which revealed that some of these precursors are of unexpected length. The alignment of HYL1-dependent miRNA precursors to A. thaliana genomic sequences indicated the presence of introns in 12 out of 20 genes studied. Some of the characterized intron-containing pri-miRNAs undergo alternative splicing such as exon skipping or usage of alternative 5' splice sites suggesting that this process plays a role in the regulation of miRNA biogenesis. In the hyl1 mutant intron-containing pri-miRNAs accumulate alongside spliced pri-miRNAs suggesting the recruitment of HYL1 into the miRNA precursor maturation pathway before their splicing occurs.

  8. PRI-Modeler: extracting RNA structural elements from PDB files of protein-RNA complexes.

    Science.gov (United States)

    Han, Kyungsook; Nepal, Chirag

    2007-05-01

    A complete understanding of protein and RNA structures and their interactions is important for determining the binding sites in protein-RNA complexes. Computational approaches exist for identifying secondary structural elements in proteins from atomic coordinates. However, similar methods have not been developed for RNA, due in part to the very limited structural data so far available. We have developed a set of algorithms for extracting and visualizing secondary and tertiary structures of RNA and for analyzing protein-RNA complexes. These algorithms have been implemented in a web-based program called PRI-Modeler (protein-RNA interaction modeler). Given one or more protein data bank files of protein-RNA complexes, PRI-Modeler analyzes the conformation of the RNA, calculates the hydrogen bond (H bond) and van der Waals interactions between amino acids and nucleotides, extracts secondary and tertiary RNA structure elements, and identifies the patterns of interactions between the proteins and RNAs. This paper presents PRI-Modeler and its application to the hydrogen bond and van der Waals interactions in the most representative set of protein-RNA complexes. The analysis reveals several interesting interaction patterns at various levels. The information provided by PRI-Modeler should prove useful for determining the binding sites in protein-RNA complexes. PRI-Modeler is accessible at http://wilab.inha.ac.kr/primodeler/, and supplementary materials are available in the analysis results section at http://wilab.inha.ac.kr/primodeler/.

  9. Subnuclear compartmentalization of transiently expressed polyadenylated pri-microRNAs: processing at transcription sites or accumulation in SC35 foci.

    Science.gov (United States)

    Pawlicki, Jan M; Steitz, Joan A

    2009-02-01

    MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate expression of their target messenger RNAs. We recently demonstrated that primary miRNA transcripts (pri-miRNAs) retained at transcription sites are processed with enhanced efficiency, suggesting that pri-miRNA processing is coupled to transcription in mammalian cells. We also observed that transiently expressed pri-miRNAs accumulate in nuclear foci with splicing factor SC35 and Microprocessor components, Drosha and DGCR8. Here, we show that pri-miRNAs containing a self-cleaving hepatitis delta ribozyme accumulate in the nucleoplasm after release from their transcription sites, but are not efficiently processed. Pri-miRNAs with ribozyme-generated 3' ends do not localize to SC35-containing foci, whereas cleaved and polyadenylated pri-miRNA transcripts with or without the pre-miRNA hairpin do. Pri-miRNA/SC35 foci contain a number of proteins normally associated with SC35 domains, including ASF/SF2, PABII, and the prolyl isomerase, Pin1. In contrast, RNA polymerase II and PM/Scl-100 do not strongly colocalize with pri-miRNAs in SC35-containing foci. These data argue that pri-miRNA/SC35-containing foci are not major sites of pri-miRNA processing and that pri-miRNA processing is coupled to transcription. We discuss the implications of our findings relative to recent insights into miRNA biogenesis, mRNA metabolism, and the nuclear organization of gene expression.

  10. Fluorescence, PRI and canopy temperature for water stress detection in cereal crops

    Science.gov (United States)

    Panigada, C.; Rossini, M.; Meroni, M.; Cilia, C.; Busetto, L.; Amaducci, S.; Boschetti, M.; Cogliati, S.; Picchi, V.; Pinto, F.; Marchesi, A.; Colombo, R.

    2014-08-01

    Narrow-band multispectral remote sensing techniques and thermal imagery were investigated for water stress detection in cereal crops. Visible and near infrared AISA Eagle (Specim, Finland) and thermal AHS-160 (Sensytech Inc., USA) imageries were acquired with an airborne survey on a farm-level experimental site where maize (Zea mays L.) and sorghum (Sorghum bicolor L.) were grown with three different irrigation treatments. Vegetation biophysical and eco-physiological measurements were collected concurrently with the airborne campaign. Leaf fluorescence yield (ΔF/Fm‧) resulted to be a good indirect measure of water stress. Therefore, ΔF/Fm‧ measurements were compared against remotely sensed indicators: (i) the Photochemical Reflectance Index (PRI), (ii) the sun-induced chlorophyll fluorescence at 760 nm (F760), retrieved by the Fraunhofer line depth method and (iii) the canopy temperature (TC) calculated decoupling soil and vegetation contributions. TC was related to ΔF/Fm‧ with the highest determination coefficient (R2 = 0.65), followed by PRI586 (reference band at 586 nm) (R2 = 0.51). The relationship with F760 was significant but weaker (R2 = 0.36). The coefficient of determination increased up to 0.54 when pigment concentration was considered by multiplying ΔF/Fm‧ and chlorophyll content, confirming the close relationship between passive fluorescence signal, pigment content and light photosystem efficiency. PRI586, F760 and TC maps were produced in maize and sorghum plots. The differences in the average values of PRI586, F760 and TC extracted from the plots with different water treatments showed that water treatments were well discriminated in maize plots by the three remotely sensed indicators. This was confirmed by the visual observation of the PRI586, F760 and TC maps, while in sorghum plots, F760 and TC appeared more sensitive to water stress compared to PRI586.

  11. 7 CFR 14.6 - Criteria for determining the pri- mary purpose of payments with respect to potential exclusion...

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Criteria for determining the pri- mary purpose of payments with respect to potential exclusion from gross income. 14.6 Section 14.6 Agriculture Office of the... Criteria for determining the pri- mary purpose of payments with respect to potential exclusion from...

  12. Bidirectional processing of pri-miRNAs with branched terminal loops by Arabidopsis Dicer-like1.

    Science.gov (United States)

    Zhu, Hongliang; Zhou, Yuyi; Castillo-González, Claudia; Lu, Amber; Ge, Chunxiao; Zhao, Ying-Tao; Duan, Liusheng; Li, Zhaohu; Axtell, Michael J; Wang, Xiu-Jie; Zhang, Xiuren

    2013-09-01

    MicroRNAs (miRNAs) originate from primary transcripts (pri-miRNAs) with characteristic stem-loop structures, and their accurate processing is required for the production of functional miRNAs. Here, using the pri-miR-166 family in Arabidopsis thaliana as a paradigm, we report the crucial role of pri-miRNA terminal loops in miRNA biogenesis. We found that multibranched terminal loops in pri-miR-166s substantially suppress miR-166 expression in vivo. Unlike canonical processing of pri-miRNAs, terminal loop-branched pri-miRNAs can be processed by Dicer-like 1 (DCL1) complexes bidirectionally from base to loop and from loop to base, resulting in productive and abortive processing of miRNAs, respectively. In both cases, DCL1 complexes canonically cut pri-miRNAs at a distance of 16-17 bp from a reference single-stranded loop region. DCL1 also adjusts processing sites toward an internal loop through its helicase domain. These results provide new insight into the poorly understood processing mechanism of pri-miRNAs with complex secondary structures.

  13. PriFi - Using a Multiple Alignment of Related Sequences to Find Primers for  Amplification of Homologs

    DEFF Research Database (Denmark)

    Fredslund, Jakob; Schauser, Leif; Madsen, Lene Heegaard;

    2005-01-01

    Using a comparative approach, the web program PriFi (http://cgi-www.daimi.au.dk/cgi-chili/PriFi/main) designs pairs of primers useful for PCR amplification of genomic DNA in species where prior sequence information is not available. The program works with an alignment of DNA sequences from...

  14. Functional interactions between polypyrimidine tract binding protein and PRI peptide ligand containing proteins.

    Science.gov (United States)

    Coelho, Miguel B; Ascher, David B; Gooding, Clare; Lang, Emma; Maude, Hannah; Turner, David; Llorian, Miriam; Pires, Douglas E V; Attig, Jan; Smith, Christopher W J

    2016-08-15

    Polypyrimidine tract binding protein (PTBP1) is a heterogeneous nuclear ribonucleoprotein (hnRNP) that plays roles in most stages of the life-cycle of pre-mRNA and mRNAs in the nucleus and cytoplasm. PTBP1 has four RNA binding domains of the RNA recognition motif (RRM) family, each of which can bind to pyrimidine motifs. In addition, RRM2 can interact via its dorsal surface with proteins containing short peptide ligands known as PTB RRM2 interacting (PRI) motifs, originally found in the protein Raver1. Here we review our recent progress in understanding the interactions of PTB with RNA and with various proteins containing PRI ligands.

  15. Drevesno preiskovanje Monte Carlo s Thompsonovim vzorčenjem pri igri Prebivalci otoka Catan

    OpenAIRE

    2016-01-01

    Drevesno preiskovanje Monte Carlo (MCTS) je ena izmed najbolj uporabljenih metod pri implementaciji močnega računalniškega igralca iger v umetni inteligenci, brez uporabe predhodnega znanja o domeni. Najmočnejši in najbolj popularni algoritmi, ki se pogosto uporabljajo za rešitev t.i. dileme raziskovanja (engl. exploration) proti izkoriščanju znanja (engl. exploitation) pri problemu več-rokih banditov, so raziskani in predstavljeni s pomočjo pregleda literature. Na podlagi empiričnih študij T...

  16. Tertiary structure mapping of the pri-miRNA miR-17~92.

    Science.gov (United States)

    Chaulk, Steven G; Fahlman, Richard P

    2014-01-01

    The understanding of RNA in regulating gene expression has exploded over the past 15 years. MicroRNAs (miRNAs) have vastly expanded the role of RNA in gene regulation beyond spliceosomal, ribosomal, and messenger RNAs. Approximately one half of miRNAs are polycistronic, where two or more miRNAs are encoded on a single pri-miRNA transcript, termed a miRNA cluster. The six miRNAs of the miR-17~92 cluster are contained within a ~800 nucleotide region within intron 3 of the cl13orf25 ~7 kb pri-miRNA transcript. We recently reported on the tertiary structured domain of miR-17~92 and its role in modulating miRNA biogenesis. The key finding was that the cluster structure explained the differential processing of the miRNA hairpins by Drosha. This work demonstrated the need to consider pri-miRNA tertiary structure in miRNA biogenesis. Since biochemical structure probing is typically performed on relatively short RNAs (≤200 nucleotides), we had to adapt these methodologies for application on large RNAs (~800 nucleotide miR-17~92 pri-miRNA). We present here our adaptation of a protection footprinting method using ribonucleases to probe the structure of the ~800 nucleotide miR-17~92 pri-miRNA. We outline the technical difficulties involved in probing large RNAs and data visualization using denaturing polyacrylamide gel electrophoresis and how we adapted the existing approaches to probe large RNAs. The methodology outlined here is generally applicable to large RNAs including long noncoding RNAs (lncRNA).

  17. Dimeric structure of the N-terminal domain of PriB protein from Thermoanaerobacter tengcongensis solved ab initio.

    Science.gov (United States)

    Liebschner, Dorothee; Brzezinski, Krzysztof; Dauter, Miroslawa; Dauter, Zbigniew; Nowak, Marta; Kur, Józef; Olszewski, Marcin

    2012-12-01

    PriB is one of the components of the bacterial primosome, which catalyzes the reactivation of stalled replication forks at sites of DNA damage. The N-terminal domain of the PriB protein from the thermophilic bacterium Thermoanaerobacter tengcongensis (TtePriB) was expressed and its crystal structure was solved at the atomic resolution of 1.09 Å by direct methods. The protein chain, which encompasses the first 104 residues of the full 220-residue protein, adopts the characteristic oligonucleotide/oligosaccharide-binding (OB) structure consisting of a five-stranded β-barrel filled with hydrophobic residues and equipped with four loops extending from the barrel. In the crystal two protomers dimerize, forming a six-stranded antiparallel β-sheet. The structure of the N-terminal OB domain of T. tengcongensis shows significant differences compared with mesophile PriBs. While in all other known structures of PriB a dimer is formed by two identical OB domains in separate chains, TtePriB contains two consecutive OB domains in one chain. However, sequence comparison of both the N-terminal and the C-terminal domains of TtePriB suggests that they have analogous structures and that the natural protein possesses a structure similar to a dimer of two N-terminal domains.

  18. Association of a peptoid ligand with the apical loop of pri-miR-21 inhibits cleavage by Drosha.

    Science.gov (United States)

    Diaz, Jason P; Chirayil, Rachel; Chirayil, Sara; Tom, Martin; Head, Katie J; Luebke, Kevin J

    2014-04-01

    We have found a small molecule that specifically inhibits cleavage of a precursor to the oncogenic miRNA, miR-21, by the microprocessor complex of Drosha and DGCR8. We identified novel ligands for the apical loop of this precursor from a screen of 14,024 N-substituted oligoglycines (peptoids) in a microarray format. Eight distinct compounds with specific affinity were obtained, three having affinities for the targeted loop in the low micromolar range and greater than 15-fold discrimination against a closely related hairpin. One of these compounds completely inhibits microprocessor cleavage of a miR-21 primary transcript at concentrations at which cleavage of another miRNA primary transcript, pri-miR-16, is little affected. The apical loop of pri-miR-21, placed in the context of pri-miR-16, is sufficient for inhibition of microprocessor cleavage by the peptoid. This compound also inhibits cleavage of pri-miR-21 containing the pri-miR-16 apical loop, suggesting an additional site of association within pri-miR-21. The reported peptoid is the first example of a small molecule that inhibits microprocessor cleavage by binding to the apical loop of a pri-miRNA.

  19. Dimeric structure of the N-terminal domain of PriB protein from Thermoanaerobacter tengcongensis solved ab initio

    Energy Technology Data Exchange (ETDEWEB)

    Liebschner, Dorothee [National Cancer Institute, Argonne National Laboratory, Argonne, IL 60439 (United States); Brzezinski, Krzysztof [National Cancer Institute, Argonne National Laboratory, Argonne, IL 60439 (United States); University of Bialystok, 15-399 Bialystok (Poland); Dauter, Miroslawa [Argonne National Laboratory, Argonne, IL 60439 (United States); Dauter, Zbigniew, E-mail: dauter@anl.gov [National Cancer Institute, Argonne National Laboratory, Argonne, IL 60439 (United States); Nowak, Marta; Kur, Józef; Olszewski, Marcin, E-mail: dauter@anl.gov [Gdansk University of Technology, 80-952 Gdansk (Poland); National Cancer Institute, Argonne National Laboratory, Argonne, IL 60439 (United States)

    2012-12-01

    The N-terminal domain of the PriB protein from the thermophilic bacterium T. tengcongensis (TtePriB) was expressed and its crystal structure has been solved at the atomic resolution of 1.09 Å by direct methods. PriB is one of the components of the bacterial primosome, which catalyzes the reactivation of stalled replication forks at sites of DNA damage. The N-terminal domain of the PriB protein from the thermophilic bacterium Thermoanaerobacter tengcongensis (TtePriB) was expressed and its crystal structure was solved at the atomic resolution of 1.09 Å by direct methods. The protein chain, which encompasses the first 104 residues of the full 220-residue protein, adopts the characteristic oligonucleotide/oligosaccharide-binding (OB) structure consisting of a five-stranded β-barrel filled with hydrophobic residues and equipped with four loops extending from the barrel. In the crystal two protomers dimerize, forming a six-stranded antiparallel β-sheet. The structure of the N-terminal OB domain of T. tengcongensis shows significant differences compared with mesophile PriBs. While in all other known structures of PriB a dimer is formed by two identical OB domains in separate chains, TtePriB contains two consecutive OB domains in one chain. However, sequence comparison of both the N-terminal and the C-terminal domains of TtePriB suggests that they have analogous structures and that the natural protein possesses a structure similar to a dimer of two N-terminal domains.

  20. Regulation of pri-miRNA processing by a long noncoding RNA transcribed from an ultraconserved region.

    Science.gov (United States)

    Liz, Julia; Portela, Anna; Soler, Marta; Gómez, Antonio; Ling, Hui; Michlewski, Gracjan; Calin, George A; Guil, Sònia; Esteller, Manel

    2014-07-03

    Noncoding RNAs (ncRNAs) control cellular programs by affecting protein-coding genes, but evidence increasingly points to their involvement in a network of ncRNA-ncRNA interactions. Here, we show that a long ncRNA, Uc.283+A, controls pri-miRNA processing. Regulation requires complementarity between the lower stem region of the pri-miR-195 transcript and an ultraconserved sequence in Uc.283+A, which prevents pri-miRNA cleavage by Drosha. Mutation of the site in either RNA molecule uncouples regulation in vivo and in vitro. We propose a model in which lower-stem strand invasion by Uc.283+A impairs microprocessor recognition and efficient pri-miRNA cropping. In addition to identifying a case of RNA-directed regulation of miRNA biogenesis, our study reveals regulatory networks involving different ncRNA classes of importance in cancer.

  1. Construction of Pri-miR-20a Lentiviral Expression Vector%携带pri-mir-20a的慢病毒表达载体的构建

    Institute of Scientific and Technical Information of China (English)

    马清华; 徐艳敏; 武佳; 李伟; 钱程; 刘立

    2010-01-01

    采用PCR扩增获得的pri-mir-20a,过渡质粒pcDNA3.0-H1经BamH Ⅰ与Hin dⅢ双酶切后将两者连接产生pcDNA3.0-H1-pri-mir-20a重组质粒,再将PCR获得的H1-pri-mir-20a克隆到慢病毒载体pdc-SEW上获得重组慢病毒载体pdcH1-pri-mir-20a-SEW.将pCMV 8.91、pMD2.VSV.G、pdcH1-pri-mir-20a-SEW三质粒共转染293FT细胞,结果显示48 h后绿色荧光蛋白的表达达到80%,表明已成功构建出携带pri-mir-20a的慢病毒表达载体.该载体的成功构建为深入开展对miR-20a的生物学功能和靶标验证提供了有效的工具.

  2. Remote detection of water stress conditions via a diurnal photochemical reflectance index (PRI) improves yield prediction in rainfed wheat

    Science.gov (United States)

    Magney, T. S.; Vierling, L. A.; Eitel, J.

    2014-12-01

    Employing remotely sensed techniques to quantify the existence and magnitude of midday photosynthetic downregulation using the photochemical reflectance index (PRI) may reveal new information about plant responses to abiotic stressors in space and time. However, the interpretation and application of the PRI can be confounded because of its sensitivity to several variables changing at the diurnal (e.g., irradiation, shadow fraction) and seasonal (e.g., leaf area, chlorophyll and carotene pigment concentrations, irradiation) time scales. We explored different techniques to correct the PRI for variations in canopy structure and relative chlorophyll content (ChlR) using highly temporally resolved (frequency = five minutes) in-situ radiometric measurements of PRI and the Normalized Difference Vegetation Index (NDVI) over eight soft white spring wheat (Triticum aestivum L.)field plots under varying nitrogen and soil water conditions over two seasons. Our results suggest that the influence of seasonal variation in canopy ChlR and LAI on the diurnally measured PRI (PRIdiurnal) can be minimized using simple correction techniques, therefore improving the strength of PRI as a tool to quantify abiotic stressors such as daily changes in soil volumetric water content (SVWC), and vapor pressure deficit (VPD). PRIdiurnal responded strongly to available nitrogen, and linearly tracked seasonal changes in SVWC, VPD, and stomatal conductance (gc). Utilizing the PRI as an indicator of stress, yield predictions significantly over greenness indices such as the NDVI. This study provides insight towards the future interpretation and scaling of PRI to quantify rapid changes in photosynthesis, and as an indicator of plant stress.

  3. A central role for the primary microRNA stem in guiding the position and efficiency of Drosha processing of a viral pri-miRNA.

    Science.gov (United States)

    Burke, James M; Kelenis, Demetra P; Kincaid, Rodney P; Sullivan, Christopher S

    2014-07-01

    Processing of primary microRNA (pri-miRNA) stem-loops by the Drosha-DGCR8 complex is the initial step in miRNA maturation and crucial for miRNA function. Nonetheless, the underlying mechanism that determines the Drosha cleavage site of pri-miRNAs has remained unclear. Two prevalent but seemingly conflicting models propose that Drosha-DGCR8 anchors to and directs cleavage a fixed distance from either the basal single-stranded (ssRNA) or the terminal loop. However, recent studies suggest that the basal ssRNA and/or the terminal loop may influence the Drosha cleavage site dependent upon the sequence/structure of individual pri-miRNAs. Here, using a panel of closely related pri-miRNA variants, we further examine the role of pri-miRNA structures on Drosha cleavage site selection in cells. Our data reveal that both the basal ssRNA and terminal loop influence the Drosha cleavage site within three pri-miRNAs, the Simian Virus 40 (SV40) pri-miRNA, pri-miR-30a, and pri-miR-16. In addition to the flanking ssRNA regions, we show that an internal loop within the SV40 pri-miRNA stem strongly influences Drosha cleavage position and efficiency. We further demonstrate that the positions of the internal loop, basal ssRNA, and the terminal loop of the SV40 pri-miRNA cooperatively coordinate Drosha cleavage position and efficiency. Based on these observations, we propose that the pri-miRNA stem, defined by internal and flanking structural elements, guides the binding position of Drosha-DGCR8, which consequently determines the cleavage site. This study provides mechanistic insight into pri-miRNA processing in cells that has numerous biological implications and will assist in refining Drosha-dependent shRNA design.

  4. VARNOSTNA TVEGANJA PRI RAZVOJU INFORMACIJSKIH REŠITEV S POMOČJO SISTEMOV ZA UPRAVLJANJE VSEBIN

    OpenAIRE

    Leskovar, Uroš

    2015-01-01

    V diplomskem delu je obravnavana problematika varnostnih tveganj pri razvoju in uporabi informacijskih rešitev kadar uporabljamo sisteme za upravljanje vsebin. Varnost v informacijskih sistemih je pogosto ogrožena, kljub vedno novejšim tehnologijam. Diplomsko delo zajema splošen opis treh najpogostejših sistemov za upravljanje vsebin, primerjavo preteklih testiranj sistemov, varnostno testiranje s pomočjo navodil drugih organizacij in pregled najpogostejših nevarnosti. Ugotovljeno je bilo, da...

  5. Pri-miR-124 rs531564 polymorphism and colorectal cancer risk.

    Science.gov (United States)

    Gao, Xue-Ren; Wang, Hui-Ping; Zhang, Shu-Long; Wang, Ming-Xi; Zhu, Zhan-Sheng

    2015-10-01

    MiR-124 functions as a tumor suppressor and plays an important role in tumorigenesis. A common polymorphism (rs531564, C>G) in the pri-miR-124 has been recently studied in connection with cancer risk. The aim of the present study was to investigate the association between pri-miR-124 rs531564 polymorphism and the risk and clinicopathological characteristics of colorectal cancer (CRC). Two case-control studies involving 900 CRC patients and 1110 cancer-free controls showed that pri-miR-124 rs531564 polymorphism was significantly associated with the decreased risk of CRC in Xuzhou population [GG vs. CC: OR = 0.25, 95%CI = 0.09-0.67, P = 0.003; (CG+GG) vs. CC: OR = 0.73, 95%CI = 0.56-0.94, P = 0.01; GG vs. (CC+CG): OR = 0.27, 95%CI = 0.10-0.70, P = 0.004; G vs. C: OR = 0.70, 95%CI = 0.56-0.89, P = 0.003], Bengbu population [GG vs. CC: OR = 0.20, 95%CI = 0.04-0.90, P = 0.02; GG vs. (CC+CG): OR = 0.21, 95%CI = 0.05-0.95, P = 0.03; G vs. C: OR = 0.72, 95%CI = 0.54-0.98, P = 0.03] and pooled population [GG vs. CC: OR = 0.26, 95%CI = 0.11-0.59, Ppri-miR-124 rs531564 polymorphism was significantly associated with the decreased risk of poor differentiation and lymph node metastasis of CRC. Our results suggest that pri-miR-124 rs531564 polymorphism may be a genetic modifier for developing CRC. However, further studies are needed to validate our findings.

  6. Stimulation of pri-miR-18a Processing by hnRNP A1.

    Science.gov (United States)

    Michlewski, Gracjan; Guil, Sonia; Cáceres, Javier F

    2011-01-01

    Recent evidence suggests that the canonical miRNA processing pathway can be regulated by a number of positive and negative trans-acting factors. This chapter provides an overview of hnRNP Al-mediated regulation of miR-18a biogenesis. Our laboratory has recently established that the multifunctional RNA-binding protein hnRNP Al is required for the processing of miR-18a at the nuclear step of Drosha-mediated processing. By combining structural and functional analysis of RNA, we showed that hnRNP Al regulates the processing of pri-miR-18a by binding to its terminal loop and reshaping its stem-loop structure, thus allowing for a more effective Drosha cleavage. Furthermore, we linked the event of hnRNP A1-binding to the pri-miR-18a with an unusual phylogenetic sequence conservation of its terminal loop. Bioinformatic and mutational analysis revealed that a number of pri-miRNAs have highly conserved terminal loops, which are predicted to act as landing pads for trans-acting factors influencing miRNA processing. These results underscore a previously uncharacterized role for general RNA-binding proteins as factors that facilitate the processing of specific miRNAs, revealing an additional level of complexity for the regulation of miRNA production and function.

  7. Remote sensing of ecosystem light use efficiency with MODIS-based PRI - the DOs and DON'Ts

    Science.gov (United States)

    Goerner, A.; Reichstein, M.; Tomelleri, E.; Hanan, N.; Rambal, S.; Papale, D.; Dragoni, D.; Schmullius, C.

    2010-09-01

    Several studies sustained the possibility that a photochemical reflectance index (PRI) directly obtained from satellite data can be used as a proxy for ecosystem light use efficiency (LUE) in diagnostic models of gross primary productivity. This modelling approach would avoid the complications that are involved in using meteorological data as constraints for a fixed maximum LUE. However, no unifying model predicting LUE across climate zones and time based on MODIS PRI has been published to date. In this study, we evaluate the efficiency with which MODIS-based PRI can be used to estimate ecosystem light use efficiency at study sites of different plant functional types and vegetation densities. Our objective is to examine if known limitations such as dependance on viewing and illumination geometry can be overcome and a single PRI-based model of LUE (i.e. based on the same reference band) can be applied under a wide range of conditions. Furthermore, we were interested in the effect of using different faPAR (fraction of absorbed photosynthetically active radiation) products on the in-situ LUE used as ground truth and thus on the whole evaluation exercise. We found that estimating LUE at site-level based on PRI reduces uncertainty compared to the approaches relying on a maximum LUE reduced by minimum temperature and vapour pressure deficit. Despite the advantages of using PRI to estimate LUE at site-level, we could not establish an universally applicable light use efficiency model based on MODIS PRI. Models that were optimised for a pool of data from several sites did not perform well.

  8. mirTarPri: improved prioritization of microRNA targets through incorporation of functional genomics data.

    Directory of Open Access Journals (Sweden)

    Peng Wang

    Full Text Available MicroRNAs (miRNAs are a class of small (19-25 nt non-coding RNAs. This important class of gene regulator downregulates gene expression through sequence-specific binding to the 3'untranslated regions (3'UTRs of target mRNAs. Several computational target prediction approaches have been developed for predicting miRNA targets. However, the predicted target lists often have high false positive rates. To construct a workable target list for subsequent experimental studies, we need novel approaches to properly rank the candidate targets from traditional methods. We performed a systematic analysis of experimentally validated miRNA targets using functional genomics data, and found significant functional associations between genes that were targeted by the same miRNA. Based on this finding, we developed a miRNA target prioritization method named mirTarPri to rank the predicted target lists from commonly used target prediction methods. Leave-one-out cross validation has proved to be successful in identifying known targets, achieving an AUC score up to 0. 84. Validation in high-throughput data proved that mirTarPri was an unbiased method. Applying mirTarPri to prioritize results of six commonly used target prediction methods allowed us to find more positive targets at the top of the prioritized candidate list. In comparison with other methods, mirTarPri had an outstanding performance in gold standard and CLIP data. mirTarPri was a valuable method to improve the efficacy of current miRNA target prediction methods. We have also developed a web-based server for implementing mirTarPri method, which is freely accessible at http://bioinfo.hrbmu.edu.cn/mirTarPri.

  9. Remote sensing of ecosystem light use efficiency with MODIS-based PRI – the DOs and DON'Ts

    Directory of Open Access Journals (Sweden)

    A. Goerner

    2010-09-01

    Full Text Available Several studies sustained the possibility that a photochemical reflectance index (PRI directly obtained from satellite data can be used as a proxy for ecosystem light use efficiency (LUE in diagnostic models of gross primary productivity. This modelling approach would avoid the complications that are involved in using meteorological data as constraints for a fixed maximum LUE. However, no unifying model predicting LUE across climate zones and time based on MODIS PRI has been published to date. In this study, we evaluate the efficiency with which MODIS-based PRI can be used to estimate ecosystem light use efficiency at study sites of different plant functional types and vegetation densities. Our objective is to examine if known limitations such as dependance on viewing and illumination geometry can be overcome and a single PRI-based model of LUE (i.e. based on the same reference band can be applied under a wide range of conditions. Furthermore, we were interested in the effect of using different faPAR (fraction of absorbed photosynthetically active radiation products on the in-situ LUE used as ground truth and thus on the whole evaluation exercise. We found that estimating LUE at site-level based on PRI reduces uncertainty compared to the approaches relying on a maximum LUE reduced by minimum temperature and vapour pressure deficit. Despite the advantages of using PRI to estimate LUE at site-level, we could not establish an universally applicable light use efficiency model based on MODIS PRI. Models that were optimised for a pool of data from several sites did not perform well.

  10. Prion-like nanofibrils of small molecules (PriSM): A new frontier at the intersection of supramolecular chemistry and cell biology.

    Science.gov (United States)

    Zhou, Jie; Du, Xuewen; Xu, Bing

    2015-01-01

    Formed by non-covalent interactions and not defined at genetic level, the assemblies of small molecules in biology are complicated and less explored. A common morphology of the supramolecular assemblies of small molecules is nanofibrils, which coincidentally resembles the nanofibrils formed by proteins such as prions. So these supramolecular assemblies are termed as prion-like nanofibrils of small molecules (PriSM). Emerging evidence from several unrelated fields over the past decade implies the significance of PriSM in biology and medicine. This perspective aims to highlight some recent advances of the research on PriSM. This paper starts with description of the intriguing similarities between PriSM and prions, discusses the paradoxical features of PriSM, introduces the methods for elucidating the biological functions of PriSM, illustrates several examples of beneficial aspects of PriSM, and finishes with the promises and current challenges in the research of PriSM. We anticipate that the research of PriSM will contribute to the fundamental understanding at the intersection of supramolecular chemistry and cell biology and ultimately lead to a new paradigm of molecular (or supramolecular) therapeutics for biomedicine.

  11. PRI-CAT: a web-tool for the analysis, storage and visualization of plant ChIP-seq experiments.

    Science.gov (United States)

    Muiño, Jose M; Hoogstraat, Marlous; van Ham, Roeland C H J; van Dijk, Aalt D J

    2011-07-01

    Although several tools for the analysis of ChIP-seq data have been published recently, there is a growing demand, in particular in the plant research community, for computational resources with which such data can be processed, analyzed, stored, visualized and integrated within a single, user-friendly environment. To accommodate this demand, we have developed PRI-CAT (Plant Research International ChIP-seq analysis tool), a web-based workflow tool for the management and analysis of ChIP-seq experiments. PRI-CAT is currently focused on Arabidopsis, but will be extended with other plant species in the near future. Users can directly submit their sequencing data to PRI-CAT for automated analysis. A QuickLoad server compatible with genome browsers is implemented for the storage and visualization of DNA-binding maps. Submitted datasets and results can be made publicly available through PRI-CAT, a feature that will enable community-based integrative analysis and visualization of ChIP-seq experiments. Secondary analysis of data can be performed with the aid of GALAXY, an external framework for tool and data integration. PRI-CAT is freely available at http://www.ab.wur.nl/pricat. No login is required.

  12. [Effects of N, K fertilization on the relationship between photosynthetic light use efficiency and photochemical reflectance index (PRI)].

    Science.gov (United States)

    Wu, Chao-Yang; Niu, Zheng; Tang, Quan; Huang, Wen-Jiang

    2009-02-01

    PRI (Photochemical reflectance index) has provided a fast and reliable method for estimating photosynthetic light use efficiency across species. Increasing efforts have been paid to explore the effects of such disturbances as water content and CO2 concentration on the relationship between PRI and LUE. In the present paper, five types of wheat with different nitrogen and kalium fertilization were selected to study the influence of varied fertilization levels on the relationship between PRI and LUE. The results proved that leaf chlorophyll contents as well as canopy PRI increased with the increase in nitrogen and kalium fertilization. For all the nitrogen and kalium fertilization of wheat, the regression coefficients R2 are 0.710 4 and 0.853 4 respectively. When considering different levels of fertilization, the regression coefficients R2 are 0.602 0, 0.640 4 and 0.801 4 for three types of nitrogen fertilization, and 0.379 1, 0.640 4 and 0.676 9 for kalium fertilization. Therefore, PRI not only can be a reliable indicator of LUE but also can reflect the fertilization situation of wheat with different precisions of LUE assessment which can provide important reference for management and precision agriculture.

  13. POZNAVANJE PLANETOV PRI UČENCIH NA RAZREDNI STOPNJI IZOBRAŽEVANJA

    OpenAIRE

    Makovec, Julijana

    2009-01-01

    Namen raziskave je ugotoviti poznavanje planetov med učenci nižjih razredov osnovne šole. Zanimalo nas je, koliko učenci poznajo planete po imenu in kako jih prepoznajo na podlagi slikovnega gradiva. V nadaljevanju smo želeli raziskati predstave učencev o planetih in njihovih lastnostih. Pri tem nas je zanimalo, ali učenci poznajo velikosti planetov in razmere na njih in ali ločijo planete od zvezd in naravnih satelitov. Na koncu pa smo preverili še, kaj učenci vedo o opazovanju planetov. Ugo...

  14. Vías de señalización en enfermedades priónicas

    OpenAIRE

    2007-01-01

    Las enfermedades priónicas, también conocidas como EETs (Encefalopatías Espongiformes Transmisibles), son un grupo de enfermedades neurodegenerativas fatales que, debido a su transmisibilidad potencial han tenido un gran impacto social, político y económico en las últimas décadas. Las EETs afectan a los humanos y a algunos grupos de animales, presentan largos períodos de incubación y producen la muerte pocos meses después de la aparición de la sintomatología clínica. Su origen se atribuye a ...

  15. Enfermedades priónicas: riesgos y medidas preventivas en Odontología

    OpenAIRE

    2015-01-01

    El objetivo del presente trabajo es hacer una revisión de los últimos trabajos de investigación sobre las enfermedades priónicas para poder actualizar y transmitir información sobre las medidas de prevención y bioseguridad de estos agentes infecciosos no convencionales. Los priones son glucoproteínas situadas en las superficies celulares. Las versiones modificadas de los priones normales ocasionan enfermedades de progresión lenta, a menudo mortales del sistema nervioso central, una de las más...

  16. ZDRAVSTVENA NEGA IN PREHRANA BOLNIKA PRI KIRURŠKEM ZDRAVLJENJU TREBUŠNE SLINAVKE

    OpenAIRE

    Lulik, Mateja

    2014-01-01

    Diplomsko delo je sestavljeno iz dveh delov. V medicinskem delu je opisana anatomija, fiziologija ter bolezni trebušne slinavke. Drugi del diplomskega dela predstavlja zdravstveno nego bolnika pri kirurškem zdravljenju trebušne slinavke ter najpogostejše negovalne diagnoze po operaciji. Opisana je tudi prehrana bolnika po operaciji, najpogostejša priporočila za prehrano bolnika na domu po odpustu iz bolnišnice ter po operaciji. Diplomsko delo s pomočjo domače, tuje literature in razpoložlj...

  17. Mobing kot motnja v komunikaciji pri delu in dejavnik duševnega zdravja dostojanstva posameznika

    OpenAIRE

    Tičar, Luka

    2012-01-01

    Avtor opozarja na vsebinsko kompleksnost pojma mobing, pri čemer pa se v njegovo vsebino ne spušča, četudi je tesno in pomembno povezan s temeljno človekovo pravico do dostojanstva. Izhaja iz predpostavke, da mobing pomeni motnjo v komunikaciji med ljudmi in da je kot tak prej predmet socioloških in psiholoških znanstvenih razprav kot poglobljene pravne analize. Zato izpostavi zgolj eno opredelitev mobinga, z namenom, da bi seznanile bralca z njegovo osnovno vsebino. Pravno razpravo pa omeji ...

  18. Explaining the variability of Photochemical Reflectance Index (PRI): deconvolution of variability related to Light Use Efficiency and Canopy attributes.

    Science.gov (United States)

    Merlier, Elodie; Hmimina, Gabriel; Dufrêne, Eric; Soudani, Kamel

    2014-05-01

    The Photochemical Reflectance Index (PRI) was designed as a proxy of the state of xanthophyll cycle which is used as a response of plants to excess of light (Gamon et al., 1990; 1992). Strong relationships between PRI and LUE were shown at leaf and canopy scales and over a wide range of species (Garbulsky et al., 2011). However, its use at canopy scale was shown to be significantly hampered by effects of confounding factors such as the PRI sensitivity to leaf pigment content (Gamon et al. 2001; Nakaji et al. 2006) and to canopy structure (Hilker et al. 2008). Several approaches aimed at correcting such effects and recent works focused on the deconvolution of LUE related and LUE unrelated PRI variability (Rahimzadeh-Bajgiran et al. 2012).In this study, the PRI variability at canopy scale is investigated over two years on three species (Fagus sylvatica, Quercus robur and Pinus sylvestris) growing under two water regimes. At daily scale, PRI variability is mainly explained by radiation conditions. As already reported at leaf scale in Hmimina et al. (2014), analysis of PRI responses to incoming photosynthetically active radiation over seasonal scale allowed to separate two sources of variability : a constitutive variability mainly related to canopy structure and leaf chlorophyll content and a facultative variability mainly related to LUE and soil moisture content. These results highlight the composite nature of PRI signal measured at canopy scale and the importance of disentangling its sources of variability in order to accurately assess ecosystem light use efficiency. Gamon JA, Field CB, Bilger W, Björkman O, Fredeen AL, Peñuelas J. 1990. Remote sensing of the xanthophyll cycle and chlorophyll fluorescence in sunflower leaves and canopies. Oecologia 85, 1-7. Gamon JA, Field CB, Fredeen A AL, Thayer S. 2001. Assessing photosynthetic downregulation in sunflower stands with an optically-based model. Photosynthesis Research 67, 113-125. Gamon JA, Peñuelas J, Field CB

  19. Attachment to Caregivers and Type 1 Diabetes in Children / Navezanost Na Starše in Sladkorna Bolezen Tipa 1 Pri Otrocih

    Directory of Open Access Journals (Sweden)

    Drobnič Radobuljac Maja

    2015-03-01

    Full Text Available Navezanost je vedenjski in fiziološki sistem, ki posamezniku omogoča dinamično prilagajanje na okolje. Navezanost se razvija pri sovplivu med dojenčkom in materjo, igra pomembno vlogo pri razvoju otrokovih možgan in vpliva na kvaliteto posameznikovih socialnih odnosov vse življenje.

  20. La democracia fallida: la batalla de Carlos A. Madrazo por cambiar al PRI

    Directory of Open Access Journals (Sweden)

    Ricardo Pozas-Horcasitas

    2008-03-01

    Full Text Available El artículo analiza la llegada de Carlos Alberto Madrazo al Comité Ejecutivo Nacional del Partido Revolucionario Institucional (PRI, y su intento por reformar al partido, introduciendo prácticas de selección a través de la elección directa de los representantes en el partido, así como en las candidaturas a los cargos de elección popular, reforma que confrontó la tradición de la autoridad política vertical y la designación de candidatos a cargos de elección en México.The article analyzes Carlos Alberto Madrazo's arrival at the National Executive Committee of the Institutional Revolutionary Party (PRI, the political environment of his arrival, his attempt to reform the party by introducing selection practices through the direct election of representatives in the party and candidacies for popularly-elected posts, a reform that challenged the tradition of vertical political authority and the designation of candidates toelected positions in Mexico.

  1. A pri-miR-218 variant and risk of cervical carcinoma in Chinese women

    Directory of Open Access Journals (Sweden)

    Shi Ting-Yan

    2013-01-01

    Full Text Available Abstract Background MicroRNA (miRNA-related single nucleotide polymorphisms (SNPs may compromise miRNA binding affinity and modify mRNA expression levels of the target genes, thus leading to cancer susceptibility. However, few studies have investigated roles of miRNA-related SNPs in the etiology of cervical carcinoma. Methods In this case–control study of 1,584 cervical cancer cases and 1,394 cancer-free female controls, we investigated associations between two miR-218-related SNPs involved in the LAMB3-miR-218 pathway and the risk of cervical carcinoma in Eastern Chinese women. Results We found that the pri-miR-218 rs11134527 variant GG genotype was significantly associated with a decreased risk of cervical carcinoma compared with AA/AG genotypes (adjusted OR=0.77, 95% CI=0.63-0.95, P=0.015. However, this association was not observed for the miR-218 binding site SNP (rs2566 on LAMB3. Using the multifactor dimensionality reduction analysis, we observed some evidence of interactions of these two SNPs with other risk factors, especially age at primiparity and menopausal status, in the risk of cervical carcinoma. Conclusions The pri-miR-218 rs11134527 SNP was significantly associated with the risk of cervical carcinoma in Eastern Chinese women. Larger, independent studies are warranted to validate our findings.

  2. Assessing canopy PRI from airborne imagery to map water stress in maize

    Science.gov (United States)

    Rossini, M.; Fava, F.; Cogliati, S.; Meroni, M.; Marchesi, A.; Panigada, C.; Giardino, C.; Busetto, L.; Migliavacca, M.; Amaducci, S.; Colombo, R.

    2013-12-01

    This paper presents a method for mapping water stress in a maize field using hyperspectral remote sensing imagery. An airborne survey using AISA (Specim, Finland) was performed in July 2008 over an experimental farm in Italy. Hyperspectral data were acquired over a maize field with three different irrigation regimes. An intensive field campaign was also conducted concurrently with imagery acquisition to measure relative leaf water content (RWC), active chlorophyll fluorescence (ΔF/Fm‧), leaf temperature (Tl) and Leaf Area Index (LAI). The analysis of the field data showed that at the time of the airborne overpass the maize plots with irrigation deficits were experiencing a moderate water stress, affecting the plant physiological status (ΔF/Fm‧, difference between Tl and air temperature (Tair), and RWC) but not the canopy structure (LAI). Among the different Vegetation Indices (VIs) computed from the airborne imagery the Photochemical Reflectance Index computed using the reflectance at 570 nm as the reference band (PRI570) showed the strongest relationships with ΔF/Fm‧ (r2 = 0.76), Tl - Tair (r2 = 0.82) and RWC (r2 = 0.64) and the red-edge Chlorophyll Index (CIred-edge) with LAI (r2 = 0.64). Thus PRI has been proven to be related to water stress at early stages, before structural changes occurred.

  3. Involvement of PI3-K in neuroprotective effects of the 1,25-dihydroxyvitamin D3 analogue - PRI-2191.

    Science.gov (United States)

    Regulska, Magdalena; Leśkiewicz, Monika; Budziszewska, Bogusława; Kutner, Andrzej; Basta-Kaim, Agnieszka; Kubera, Marta; Jaworska-Feil, Lucylla; Lasoń, Władysław

    2006-01-01

    The active form of 1,25-dihydroxyvitamin D(3) prevents neuronal damage in vitro and in vivo , however, it induces also hypercalcemia and hyperphosphatemia. Side-chain-modified analogues of 1,25-dihydroxyvitamin D(3), which show low calcemic activity, may be potentially useful in the treatment of some neurodegenerative diseases. Previously, we have found that PRI-2191 more potently than 1,25-dihydroxyvitamin D(3) protects human neuroblastoma (SH-SY5Y) cells against hydrogen-peroxide-induced toxicity. In the present study, we evaluated effects of two other 1,25-dihydroxyvitamin D(3) analogues - PRI-1890 and PRI-1901 on the neuronal degeneration in the same cell model. In line with the previous study, 24-h incubation with hydrogen peroxide (0.5 mM) was toxic to cells, as evidenced by an enhanced efflux of lactate dehydrogenase into the culture medium, and these effects were prevented by PRI-1890 and PRI-1901 at concentration of 5, 50 and 500 nM. Comparing the neuroprotective effects of secosteroids, we found that all three analogues were efficient at lower concentration than 1,25-dihydroxyvitamin D(3) and among them the PRI-2191 showed the most evident concentration-dependent effect. In the second part of this study, an involvement of mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3-K), kinases which play a crucial role in neurodegenerative processes, in neuroprotective action of 1,25 dihydroxyvitamin D(3) and its the most potent analogue PRI-2191 has been investigated. The inhibitor of c-Jun N-terminal kinase (JNK)-MAPK (SP600125, 1 microM), inhibitor of p38-MAPK (SB-203580, 1 and 10 microM) and inhibitor of extracellular signal-regulated kinase (ERK)-MAPK (PD-98059, 15 and 30 microM) attenuated the hydrogen peroxide-induced toxicity. Moreover, PD-98059 (30 microM) enhanced neuroprotective effects of 1,25 dihydroxyvitamin D(3) but not that of PRI-2191. In contrast, the inhibitor of PI3-K (wortmannin, 10, 100 nM) did not affect hydrogen

  4. Explaining the variability of the photochemical reflectance index (PRI) at the canopy-scale: Disentangling the effects of phenological and physiological changes.

    Science.gov (United States)

    Merlier, Elodie; Hmimina, Gabriel; Dufrêne, Eric; Soudani, Kamel

    2015-10-01

    Assessing photosynthesis rates at the ecosystem scale and over large regions is important for tracking the global carbon cycle and remote sensing has provided new and useful approaches for performing this assessment. The photochemical reflectance index (PRI) is a good estimator of short-term light-use efficiency (LUE) at the leaf scale; however, confounding factors appear at larger temporal and spatial scales. In this study, canopy-scale PRI variability was investigated for three species (Fagus sylvatica L., Quercus robur L. and Pinus sylvestris L.) growing under contrasting soil moisture conditions. Throughout the growing season, no significant differences in chlorophyll content and in violaxanthin, antheraxanthin and zeaxanthin were found between species or treatments. The daily PRI vs PAR (photosynthetically active radiation) relationships were determined using continuous measurements obtained at high frequency throughout the entire growing season, from early spring budburst to later autumn senescence, and were used to deconvolute the physiological PRI variability related to LUE variations due to phenological variability and related to temporal changes in the biochemical and structural canopy attributes. The PRI vs PAR relationship is used to show that the canopy-scale PRI measured at low radiation depends on the chlorophyll content of the canopy. The range of PRI variations at an intra-daily scale and the dynamics of the xanthophyll pool do not vary between days, which suggests that the PRI responds to a xanthophyll ratio. The PAR values at PRI saturation are mainly related to the canopy chlorophyll content during budburst and senescence and to the soil moisture content when the chlorophyll content is no longer a limiting factor. This parameter is significantly lower in the oak species that experience less stress from variations in soil moisture and is species dependant. These results provide new insights regarding the analysis and the meaning of PRI

  5. Demonstrating FLAVOUR: Friendly Location-aware conference Assistant with priVacy Observant architectURe (Demo Paper)

    NARCIS (Netherlands)

    Muthukrishnan, Kavitha; Meratnia, Nirvana; Koprinkov, Georgi; Lijding, Maria; Havinga, Paul

    2005-01-01

    In this paper, we describe an implementation of FLAVOUR (Friendly Location-aware conference Assistant with priVacy Observant architectURe), in which people/infrastructure resources act as individual service providers offering their location as a service. By subscribing to this service, in the one ha

  6. Association between pri-miR-218 polymorphism and risk of hepatocellular carcinoma in a Han Chinese population.

    Science.gov (United States)

    Zhang, Lu-Shun; Liang, Wei-Bo; Gao, Lin-Bo; Li, Hong-Yu; Li, Li-Juan; Chen, Peng-Yu; Liu, Yun; Chen, Tian-Yi; Han, Jun-Ge; Wei, Yong-Gang; Zhang, Lin

    2012-05-01

    MicroRNAs are noncoding RNA molecules of 18-25 nucleotides that regulate gene expression at the post-transcriptional level. The aim of this study was to investigate whether pri-miR-218 rs11134527 A/G polymorphism influences the risk of hepatocellular carcinoma (HCC) or not. pri-miR-218 rs11134527 A/G was genotyped in 302 HCC patients and 513 control subjects using the polymerase chain reaction-restriction fragment length polymorphism assay. The AG genotype of pri-miR-218 rs11134527 A/G was associated with family history (p=0.018, odds ratio [OR]=2.96, 95% confidence interval [CI]: 1.16-7.56) and elevated serum α-fetoprotein (serum alpha-fetoprotein [AFP]) levels (≥20 ng/mL; p=0.009, OR=1.92, 95% CI: 1.17-3.14) in HCC patients. These findings suggested that the AG genotype of pri-miR-218 rs11134527 might relate to genetic predisposition and be involved in regulating the expression of AFP in Chinese HCC patients.

  7. Remote sensing of plant emissions of volatile isoprenoids with PRI. Prospects for upscaling (Invited)

    Science.gov (United States)

    Penuelas, J.

    2013-12-01

    Josep Peñuelas*1,2, Giovanni Marino1,2,3,4, Joan LLusia1,2, Catherine Morfopoulos1,2,5, Gerard Farre-Armengol1,2, Shawn Kefauver, Alex Guenther6 , Francesca Rapparini7 , Roger Seco1,2,6, Marc Estiarte1,2, Mónica Mejia-Chang1,2, Romà Ogaya1,2, Jordi Sardans1,2 , Andrew Turnipseed6, Peter Harley6, Osvaldo Facini7, Rita Baraldi7, Jim Greenberg6 , Iolanda Filella1,2 1 CSIC, Global Ecology Unit CREAF-CEAB-UAB, Cerdanyola del Vallés 08193, Catalonia, Spain 2 CREAF, Cerdanyola del Vallés 08193, Catalonia, Spain 3 Dipartimento di Bioscienze e Territorio, Università degli Studi del Molise, Contrada Fonte Lappone, 86090 Pesche (IS), Italy 4 Institute for Plant Protection, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy 5 Division of Ecology and Evolution, Imperial College, Silwood Park, Ascot, SL5 7PY, UK 6 Atmospheric Chemistry Division, National Center for Atmospheric Research, P.O. Box 3000, Boulder, CO 80307-3000, USA 7 Biometeorology Institute, IBIMET-CNR, Via P. Gobetti 101, Bologna, Italy Abstract Terrestrial plants re-emit around 1-2% of the carbon they fix as isoprene and monoterpenes. These emissions play major roles in the ecological relationships among living organisms and in atmospheric chemistry and climate, and yet their actual quantification at the ecosystem level in different regions is far from being resolved. Phenomenological models are used to estimate the emission rates, but the limited understanding of the function and regulation of these emissions leads to large uncertainties in such estimations. Many measurements have been made at the foliar but few at the ecosystem level, and those that do exist are limited in space and time. We here provide evidence that a simple remote sensing index, the photochemical reflectance index (PRI), which is indicative of light use efficiency (LUE), is a good indirect estimator of foliar isoprenoid emissions and therefore can be used to sense them remotely. These results open

  8. The promoter of the pri-miR-375 gene directs expression selectively to the endocrine pancreas.

    Science.gov (United States)

    Avnit-Sagi, Tali; Kantorovich, Lia; Kredo-Russo, Sharon; Hornstein, Eran; Walker, Michael D

    2009-01-01

    microRNAs (miRNAs) are known to play an essential role in controlling a broad range of biological processes including animal development. Accordingly, many miRNAs are expressed preferentially in one or a small number of cell types. Yet the mechanisms responsible for this selectivity are not well understood. The aim of this study was to elucidate the molecular basis of cell-specific expression of the pri-miR-375 gene, which is selectively expressed in pancreatic islets, and has been implicated both in the development of islets, and the function of mature pancreatic beta cells. An evolutionarily conserved 768 bp region of DNA upstream of the pri-miR-375 gene was linked to GFP and luciferase reporter genes, and expression monitored in transgenic mice and transfected cultured cells. Deletion and targeted mutagenesis analysis was used to evaluate the functional significance of sequence blocks within the upstream fragment. 5'-RACE analysis was used for mapping the pri-miR-375 gene transcription start site. The conserved 768 bp region was able to direct preferential expression of a GFP reporter gene to pancreatic islets in transgenic mice. Deletion analysis using a luciferase reporter gene in transfected cultured cell lines confirmed the cell specificity of the putative promoter region, and identified several key cis-elements essential for optimal activity, including E-boxes and a TATA sequence. Consistent with this, 5'-RACE analysis identified a transcription start site within this DNA region, 24 bp downstream of the TATA sequence. These studies define the promoter of the pri-miR-375 gene, and show that islet-specific expression of the pri-miR-375 gene is controlled at the transcriptional level. Detailed analysis of the transcriptional mechanisms controlling expression of miRNA genes will be essential to permit a comprehensive understanding of the complex role of miRNAs such as miR-375 in developmental processes.

  9. Razvoj odklanjala za rastrsko vodenje žarka pri laserskem označevanju

    OpenAIRE

    Diaci, Janez; Babnik, Aleš; Bizjak, Aleš

    2015-01-01

    Prispevek predstavlja razvoj optomehatronskega sistema, ki omogoča izdelavo rastrskih vzorcev pri laserskem označevanju. Sistem deluje tako, da v eni osi odklanjamo obdelovalni laserski žarek s pomočjo poligonskega zrcala v obliki šesterokotne prizme, ki se vrti s konstantno kotno hitrostjo. Vsak laserski blisk povzroči na površini obdelovanca označbo v obliki točke. Z odklanjanjem žarka dosežemo, da so točkaste označbe razvrščene enakomerno v ravni črti - vrstici. Pomična miza, ki je pod odk...

  10. DIDAKTIČNE IGRE PRI POUKU SPOZNAVANJA OKOLJA ZUNAJ UČILNICE

    OpenAIRE

    Majcen, Doroteja

    2014-01-01

    Didaktična igra se uporablja kot učna metoda, imeti mora vse lastnosti igre in dosegati določene (učne) cilje. Didaktična igra zunaj učilnice nudi učencu izkušnje in ob njej razvija pozitiven odnos do okolja in narave, kar je pri pouku spoznavanja okolja še posebej poudarjeno. V teoretičnem delu smo opredelili predmet spoznavanja okolja ter navedli didaktična priporočila za uresničevanje ciljev predmeta in vpeljevanje pouka spoznavanja okolja v naravo. Našteli in opisali smo didaktične strate...

  11. Patología del sueño en las enfermedades priónicas Sleep disorders in prion diseases

    OpenAIRE

    2007-01-01

    Las enfermedades priónicas son un grupo de encefalopatías con cambios neurodegenerativos causados por una proteína alterada denominada prión cuyo dato característico es la transmisibilidad. Ocurren la mayoría de las veces de forma esporádica aunque un grupo de ellas son familiares asociadas a mutaciones en el gen de la proteína priónica. El polimorfismo genético parece determinar las diferentes variantes familiares. Una de las más enigmáticas e inhabituales es el Insomnio Letal Familiar (ILF)...

  12. PRI 3.1: Electrolyte membrane fuel cells (Co-PACEM), final report (july 2002 to june 2004); PRI 3.1: Coeurs de piles a combustible a electrolyte membrane (Co-PACEM), rapport final (juillet 2002 a juin 2004)

    Energy Technology Data Exchange (ETDEWEB)

    Lamy, C.

    2004-07-01

    The researches realized in the PRI Co-PACEM aim to improve the operating of the core of the electrolyte membrane fuel cells, at low temperature in order to minimize the high voltage of the electro-chemical reactions, to decrease the cost of the membrane, to improve the properties (conductivity, mechanical and thermal stability...) and to optimize the transport of heat and reactive. The document presents the research programs. (A.L.B.)

  13. 一种 PRI 变化的雷达信号无源定位方法%A Passive Location Method of The Radar Signal with Variational PRI

    Institute of Scientific and Technical Information of China (English)

    陈晓威; 李彦志; 张国毅

    2016-01-01

    目前脉冲重复间隔(PRI)恒定的雷达信号已经越来越少,取而代之的是PRI变化的雷达信号。传统的相位差变化率定位法均假设PRI恒定,没有考虑因PRI变化引起的相位差变化率测量误差和定位误差。提出了一种利用插值法重新构造相位差数据的方法,提取了相位差变化率并进行了定位解算。仿真结果表明采用该相位差数据重构方法能够实现快速高精度的无源定位。%Currently ,radar signal with constant pulse repetition interval (PRI) is less and less ,in-stead of this is radar signal with variational PRI .Traditional location method of phase difference change rate almost supposes that PRI is constant ,hasn't considered the measuring error of phase difference rate and position error due to the change of PRI .This paper puts forward a kind of meth-od to reconstruct phase difference data by means of interpolation method ,abstracts the phase difference rate and performs location calculation .T he simulation results show that the method to reconstruct phase difference data can quickly achieve passive location with high accuracy .

  14. Responses of the reflectance indices PRI and NDVI to experimental warming and drought in European shrublands along a north–south climatic gradient

    DEFF Research Database (Denmark)

    Mänd, Pille; Hallik, Lea; Peñuelas, Josep;

    2010-01-01

    canopy reflectance, effective green leaf area index (green LAIe) and chlorophyll fluorescence of dominant species. The treatment effects on green LAIe varied among sites. We calculated three reflectance indices: photochemical reflectance index PRI [531 nm; 570 nm], normalized difference vegetation index...... NDVI680 [780 nm; 680 nm] using red spectral region, and NDVI570 [780 nm; 570 nm] using the same green spectral region as PRI. All three reflectance indices were significantly related to green LAIe and were able to detect changes in shrubland vegetation among treatments. In general warming treatment...... in vegetation areas with low green LAIe. As both soil reflectance and LAI varied between northern and southern sites it is problematic to draw universal conclusions of climate-derived changes in all vegetation types based merely on PRI measurements. We propose that canopy level PRI measurements can be more...

  15. Neki problemi pri prevođenju moderne poezije promatrani kroz teoriju konceptualne integracije

    Directory of Open Access Journals (Sweden)

    Sanja Cimer

    2011-12-01

    Full Text Available Straußeneimondaber ich finde nichts mehrnichts mehr an Buchstaben im Wortmache so ähnlichwie Luftgang ohne Seiles fällt nichts mehraber nichts ist eben auchZaungrenzeund LebenMittelstelzengangder Strauß legt seine Eier in den Himmelfast Massenproduktionkugelrunde Stundenmit Haut auf wirren ÜberzugHände greifen leerwenn nicht duaber das kann nicht –Angsttauchen mit Höhengangund die Hand als Schalewer fängt, – verliert!Mjesec od nojevog jajetaali nema ničeg višeničeg više u slovima riječičinim sličnokao hod po zraku bez konopcaništa više ne padaali i ništa jestgranica od ogradei životsredinom na štulamanoj liježe svoja jaja u nebogotovo masovna proizvodnjasati okrugli poput loptes kožom na neurednoj presvlaciruke posežu u praznoako nisi tiali to ne može –uron u strah s letom u visinei ruka poput ljusketko ulovi, – izgubi!Prevođenje poezije oduvijek se smatralo osobitom temom unutar ionako složenog područja književnog prevođenja. Budući da poezija predstavlja književni oblik u kojem su sadržaj i forma nerazdvojno povezani, jasno je zašto ona predstavlja najzahtjevniji oblik prevoditeljske aktivnosti. Problematičnu točku pri prevođenju poezije predstavlja činjenica da ona daje izrazito “zgusnutu” i kompaktnu formu te da jezik poezije uglavnom počiva na konotacijama, a ne na denotacijama. Noviji pristupi prevođenju prepoznaju da su nekadašnja protivljenja prevođenju poezije, to jest isticanja njezine neprevodivosti, zapravo bila usmjerena na nemogućnost potpunog prenošenja svih aspekata izvornog poetskog djela u ciljni jezik i njegovu kulturu i tradiciju (neprevodivost poezije isticali su, primjerice, T. S. Eliott: “[poezija je] stalni podsjetnik na sve ono što se može reći samo na jednom jeziku”, Roman Jakobson: “poezija je po definiciji neprevodiva” ili Nabokov: “pjesma ne može biti prevedena, reproducirana ni iznova oblikovana na ciljnom jeziku” (svi navedeni citati te citati

  16. ¿Por qué perdió el PRI en Yucatán? Las transiciones de este año en bastiones priístas, caída de una imagen distorsionada

    Directory of Open Access Journals (Sweden)

    Lic. Fernando Pacheco Bailón

    2001-01-01

    Full Text Available El pasado domingo 27 de mayo, a eso de las 5:30 de la tarde, los mexicanos nos enteramos a través de los medios de comunicación de televisión abierta y pagada de la ventaja que tenía el candidato a la gubernatura de Yucatán, a través de la coalición PAN, PRD, PVEM, PT, Patricio Patrón Laviada, con respecto al candidato del PRI, Orlando Paredes Lara. Más tarde, de aquella llamada telefónica que hiciera el presidente Vicente Fox para felicitar al candidato que aventajaba al del tricolor, al hacer referencia a las encuestas de salida llevadas a cabo por empresas como Consulta Mitovsky, Garo y otras más. Hay muchas metodologías para poder analizar procesos electorales, así como distintas formas de abordar el problema del por qué perdió el PRI en Yucatán. Para efectos de estudio se planteará las condiciones preelectorales que se dieron, el propio momento electoral del pasado 27 de mayo, así como la visión futura de PRI como organización política en tiempos transitorios.

  17. Pri I J K(介绍国际青年世界语者大会)

    Institute of Scientific and Technical Information of China (English)

    王瑞祥

    1994-01-01

    ■irkaǔ160 esperantistoj el 33 landoj kun granda entuziasmo partoprenis en la 49-a Interna-cia Junulara Kongreso(IJK) de la 7-a ■is la 14-a de aǔgusto 1993 en la urbo Vraca, Bulgario.La inaǔguro okazis en la Urba Artgalerio. Aliaj kunvenoj estis aran■itaj en apudurba univer-sitata kvartalo, kie la ■eestantoj ankaǔ lo■is. Tage,LKK organizis interesajn programerojn laǔ lagustoj de la junuloj. Estis diskuto pri la modernaj tendencoj en Esperanto-Komunumo, prelegoj dela"kongresa universitato", renkonti■oj de redaktoroj de junularaj gazetoj kaj de partopernantoj deIJK en la jaro 1963, kiu okazis ankaǔ en Vraca, diversaj kursoj, forumo pri Bulgario, internaci-aj ekzamenoj de UEA/ILEI kaj pluraj Esperant-lingvaj videofilm-prezentoj. Interalie, la revuo

  18. PRI变换法用于信号分选的性能研究%Research on performance of PRI transform in signal sorting

    Institute of Scientific and Technical Information of China (English)

    张什永; 张海黎; 徐龙; 胡泽宾

    2012-01-01

    Since it can suppress PRI harmonics efficiently,PRI transform,a DTOA-based algorithm,is widely researched in radar signal sorting.Phase factor is firstly discussed,and its effect in PRI spectrum accumulating is revealed;then both the limitations of PRI transform and its adapted PRI types are analyzed theoretically.Finally,the performance of PRI transform to real signal environment is given by computer simulations.Both theoretical analysis and simulation results can be used as engineering reference.%PRI变换是一种基于脉冲到达时间的信号分选方法,由于该算法能够有效抑制真实PRI的谐波,近年来受到越来越多的关注。首先讨论了相位因子的作用,揭示了相位因子是进行变换谱峰值累积的关键;接着通过理论分析指出了PRI变换法的局限和适用的PRI类型;最后仿真分析了该方法对分选过程所面临的信号环境的适应性。理论及仿真分析结果可作为工程应用的参考依据,具有一定的实用价值。

  19. Promotion of cortico-cerebral precursors expansion by artificial pri-miRNAs targeted against the Emx2 locus.

    Science.gov (United States)

    Diodato, Assunta; Pinzan, Moira; Granzotto, Marilena; Mallamaci, Antonello

    2013-04-01

    Emx2 encodes for a transcription factor controlling several aspects of cerebral cortex development. Its overexpression promotes self-renewal of young cortico-cerebral precursors, it promotes neuronal rather than gliogenic fates and it protects neuronal progenitors from cell death. These are all key activities for purposes of gene-promoted brain repair. Artificial pri-miRNAs targeting non-coding cis-active modules and/or conserved sequences of the Emx2 locus were delivered to embryonic cortico-cerebral precursors, by lentiviral vectors. A subset of these pri-miRNAs upregulated Emx2, possibly stimulating its transcription. That led to enhanced self-renewal, delayed differentiation and reduced death of neuronally committed precursors, resulting in an appreciable expansion of the neuronogenic precursors pool. This method makes Emx2 overexpression for purposes of brain repair a more feasible goal, avoiding the drawbacks of exogenous gene copies introduction. Interestingly, the two genomic enhancers targeted by these pri-miRNAs were discovered to be naturally transcribed. Their expression profile suggests their possible involvement in regulation of Emx2 transcription.

  20. Blocking the maturation of OncomiRNAs using pri-miRNA-17∼92 aptamer in retinoblastoma.

    Science.gov (United States)

    Subramanian, Nithya; Kanwar, Jagat R; Kanwar, Rupinder K; Krishnakumar, Subramanian

    2015-02-01

    The miR-17∼92. or oncomiR-1, cluster encodes oncogenic microRNAs (miRNAs), and it also promotes retinoblastoma (RB) tumor formation. Antagomir and miRNA mimics based approaches are widely tried against oncogenic and tumor suppressive miRNAs. Other methods for targeting cancer related miRNAs are still under development. In the current study, we focused on the pri-miRNA-17∼92 aptamer (pri-apt), which can potentially replace the mix of five antagomirs by one aptamer that function to abrogate the maturation of miR-17, miR-18a, and miR-19b (Ppri-apt led to S-phase arrest in WERI-Rb1 cells and onset of apoptosis in both Y79 and WERI-Rb1 cell lines. There was increased cytotoxicity as measured by lactate dehydrogenase activity in pri-apt treated Y79 cells (Ppri-apt in RB cell lines, which can be readily modified by developing appropriate vectors for the delivery of the aptamer specifically to cancer cells.

  1. Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesis.

    Science.gov (United States)

    Chaulk, Steven G; Thede, Gina L; Kent, Oliver A; Xu, Zhizhong; Gesner, Emily M; Veldhoen, Richard A; Khanna, Suneil K; Goping, Ing Swie; MacMillan, Andrew M; Mendell, Joshua T; Young, Howard S; Fahlman, Richard P; Glover, J N Mark

    2011-01-01

    MicroRNAs (miRNAs) regulate gene expression in a variety of biological pathways such as development and tumourigenesis. miRNAs are initially expressed as long primary transcripts (pri-miRNAs) that undergo sequential processing by Drosha and then Dicer to yield mature miRNAs. miR-17~92 is a miRNA cluster that encodes 6 miRNAs and while it is essential for development it also has reported oncogenic activity. To date, the role of RNA structure in miRNA biogenesis has only been considered in terms of the secondary structural elements required for processing of pri-miRNAs by Drosha. Here we report that the miR-17~92 cluster has a compact globular tertiary structure where miRNAs internalized within the core of the folded structure are processed less efficiently than miRNAs on the surface of the structure. Increased miR-92 expression resulting from disruption of the compact miR-17~92 structure results in increased repression of integrin α5 mRNA, a known target of miR-92a. In summary, we describe the first example of pri-miRNA structure modulating differential expression of constituent miRNAs.

  2. Pri-miR-17-92a transcript folds into a tertiary structure and autoregulates its processing.

    Science.gov (United States)

    Chakraborty, Saikat; Mehtab, Shabana; Patwardhan, Anand; Krishnan, Yamuna

    2012-05-01

    MicroRNAs control gene expression either by RNA transcript degradation or translational repression. Expressions of miRNAs are highly regulated in tissues, disruption of which leads to disease. How this regulation is achieved and maintained is still largely unknown. MiRNAs that reside on clustered or polycistronic transcripts represent a more complex case where individual miRNAs from a cluster are processed with different efficiencies despite being cotranscribed. To shed light on the regulatory mechanisms that might be operating in these cases, we considered the long polycistronic primary miRNA transcript pri-miR-17-92a that contains six miRNAs with diverse functions. The six miRNA domains on this cluster are differentially processed to produce varying amounts of resultant mature miRNAs in different tissues. How this is achieved is not known. We show, using various biochemical and biophysical methods coupled with mutational studies, that pri-miR-17-92a adopts a specific three-dimensional architecture that poses a kinetic barrier to its own processing. This tertiary structure could create suboptimal protein recognition sites on the pri-miRNA cluster due to higher-order structure formation.

  3. siRNA release from pri-miRNA scaffolds is controlled by the sequence and structure of RNA.

    Science.gov (United States)

    Galka-Marciniak, Paulina; Olejniczak, Marta; Starega-Roslan, Julia; Szczesniak, Michal W; Makalowska, Izabela; Krzyzosiak, Wlodzimierz J

    2016-04-01

    shmiRs are pri-miRNA-based RNA interference triggers from which exogenous siRNAs are expressed in cells to silence target genes. These reagents are very promising tools in RNAi in vivo applications due to their good activity profile and lower toxicity than observed for other vector-based reagents such as shRNAs. In this study, using high-resolution northern blotting and small RNA sequencing, we investigated the precision with which RNases Drosha and Dicer process shmiRs. The fidelity of siRNA release from the commonly used pri-miRNA shuttles was found to depend on both the siRNA insert and the pri-miR scaffold. Then, we searched for specific factors that may affect the precision of siRNA release and found that both the structural features of shmiR hairpins and the nucleotide sequence at Drosha and Dicer processing sites contribute to cleavage site selection and cleavage precision. An analysis of multiple shRNA intermediates generated from several reagents revealed the complexity of shmiR processing by Drosha and demonstrated that Dicer selects substrates for further processing. Aside from providing new basic knowledge regarding the specificity of nucleases involved in miRNA biogenesis, our results facilitate the rational design of more efficient genetic reagents for RNAi technology.

  4. A competitive regulatory mechanism discriminates between juxtaposed splice sites and pri-miRNA structures.

    Science.gov (United States)

    Mattioli, Chiara; Pianigiani, Giulia; Pagani, Franco

    2013-10-01

    We have explored the functional relationships between spliceosome and Microprocessor complex activities in a novel class of microRNAs (miRNAs), named Splice site Overlapping (SO) miRNAs, whose pri-miRNA hairpins overlap splice sites. We focused on the evolutionarily conserved SO miR-34b, and we identified two indispensable elements for recognition of its 3' splice site: a branch point located in the hairpin and a downstream purine-rich exonic splicing enhancer. In minigene systems, splicing inhibition owing to exonic splicing enhancer deletion or AG 3'ss mutation increases miR-34b levels. Moreover, small interfering-mediated silencing of Drosha and/or DGCR8 improves splicing efficiency and abolishes miR-34b production. Thus, the processing of this 3' SO miRNA is regulated in an antagonistic manner by the Microprocessor and the spliceosome owing to competition between these two machineries for the nascent transcript. We propose that this novel mechanism is commonly used to regulate the relative amount of SO miRNA and messenger RNA produced from primary transcripts.

  5. mirEX: a platform for comparative exploration of plant pri-miRNA expression data.

    Science.gov (United States)

    Bielewicz, Dawid; Dolata, Jakub; Zielezinski, Andrzej; Alaba, Sylwia; Szarzynska, Bogna; Szczesniak, Michal W; Jarmolowski, Artur; Szweykowska-Kulinska, Zofia; Karlowski, Wojciech M

    2012-01-01

    mirEX is a comprehensive platform for comparative analysis of primary microRNA expression data. RT-qPCR-based gene expression profiles are stored in a universal and expandable database scheme and wrapped by an intuitive user-friendly interface. A new way of accessing gene expression data in mirEX includes a simple mouse operated querying system and dynamic graphs for data mining analyses. In contrast to other publicly available databases, the mirEX interface allows a simultaneous comparison of expression levels between various microRNA genes in diverse organs and developmental stages. Currently, mirEX integrates information about the expression profile of 190 Arabidopsis thaliana pri-miRNAs in seven different developmental stages: seeds, seedlings and various organs of mature plants. Additionally, by providing RNA structural models, publicly available deep sequencing results, experimental procedure details and careful selection of auxiliary data in the form of web links, mirEX can function as a one-stop solution for Arabidopsis microRNA information. A web-based mirEX interface can be accessed at http://bioinfo.amu.edu.pl/mirex.

  6. 耐辐射球菌中priA类似基因突变对DNA修复和DNA转化的影响%EFFECT OF priA like GENE MUTATION ON DNA REPAIR AND DNA TRANSFORMATION OF Deinococcus radiodurans

    Institute of Scientific and Technical Information of China (English)

    王媛; 华跃进

    2012-01-01

    Replication fork progression can be blocked easily by DNA damage. Damaged DNA repair and the subsequent restart of the stalled or collapsed replication forks are critical for cell survival. The PriA-dependent pathway is the major replication restart mechanism in bacteria. To understand the roles of a priA-like gene (Dr2606) in Deinococcus radiodurans and DNA repair, a Dr2606 null mutant was constructed by replacing Dr2606 open reading frame with a kanamycin-resistanee gene and treated the Dr2606 mutant with UV and mitomycin C (MMC), and the DNA transformation efficiency of the Dr2606 mutant was also tested. Successively, the Dr2606 mutant showed a delayed growth and a dramatic decrease of cell viability. Unexpectedly, the DNA repair capability of D. radiodurans was not impaired by the inactivation of Dr2606. However, the DNA transformation efficiency was largely compromised in the mutant. These results indicate that the priA-like gene (Dr2606) is dispensable for stalled DNA replication forks restart in D. radiodurans and origin-independent replication restart in D. radiodurans may be different from other bacteria.%DNA损伤很易阻断复制叉的前进。损伤DNA的修复以及接下来停止复制叉的重启动过程对细胞生存极为重要。依赖于PriA的复制重启动机制是细菌复制重启动的主要途径。为了解priA类似基因Dr2606在耐辐射球菌中的作用,并检测Dr2606在DNA修复中的作用,本研究用卡那霉素抗性基因代替Dr2606阅读框,构建了Dr2606缺失突变株,并对突变株进行UV和丝裂霉素处理,测定了Dr2606突变株的转化效率。Dr2606的突变导致菌体生长缓慢,细胞生存率急剧下降。意外的是,耐辐射球菌的DNA修复能力没有削弱。但突变株的转化效率大大削弱。这说明在耐辐射球菌中priA类似基因Dr2606对停止复制叉的重启动过程并不是必需的;耐辐射球菌不依赖于原点的复制重启动过程可能与其他细菌不同。

  7. 基于PRI变换法的脉冲信号分选算法%Pulse Signals De-interleaving Algorithm Based on PRI Transform

    Institute of Scientific and Technical Information of China (English)

    乔宏乐; 王超; 王鹏

    2012-01-01

    脉冲重复间隔是电子对抗中信号分选的关键参数,本文研究的基于PRI变换法的信号分选算法克服了传统直方图统计法的子谐波问题,对脉冲重复间隔具有很高的估计精度,且具有很好的抗抖动性能。仿真分析表明,PRI变换法对固定PRI,抖动PRI具有很好的估计效果。%The pulse repetition interval (PRI) is a key parameter of radar signal de-interleaving in the field of elec-tronic countermeasures. The signal de-interleaving algorithm based on PRI transform overcomes the sub-harmonic problem produced in the histogram statistical method and can estimate the PRI with high precision, and possesses perfect anti - dithering capability. The simulated results indicate that PRI transform algorithm can estimate effect on fixed PRI and dithering PRI.

  8. The in-vitro antiproliferative effect of PRI-2191 and imatinib applied in combined treatment with cisplatin, idarubicin, or docetaxel on human leukemia cells.

    Science.gov (United States)

    Switalska, Marta; Nasulewicz-Goldeman, Anna; Opolska, Aleksandra; Maciejewska, Magdalena; Kutner, Andrzej; Wietrzyk, Joanna

    2012-01-01

    Imatinib mesylate (Gleevec, STI571) is a specific inhibitor of the Bcr/Abl fusion tyrosine kinase that exhibits potent antileukemic effects in chronic myelogenous leukemia. Bcr/Abl-positive K562 and Bcr/Abl-negative HL-60 human leukemia cells were used to investigate the effect of PRI-2191, a calcitriol analog, on the biological effects of imatinib combined with other anticancer drugs. The results show that PRI-2191 enhances the antiproliferative effect of imatinib on HL-60 cells. When these two agents together are applied with either docetaxel or cisplatin, but not with idarubicin, the antiproliferative effect could still be enhanced. Moreover, when the interaction between the chemotherapy agents was antagonistic or additive, PRI-2191 could even shift it to synergism. This effect correlated with an accumulation of HL-60 cells in the G0/G1 phase of the cell cycle and a decrease in the percentage of cells in the G2/M and S stage in the ternary combinations used. PRI-2191 did not influence apoptosis induced by imatinib alone or in ternary combinations with all the chemotherapy agents used. These results may suggest that the stronger antiproliferative effect of the combined treatment with PRI-2191 on HL-60 cells is related to cell cycle arrest rather than to the induction of apoptosis.

  9. No association of pri-miR-143 rs41291957 polymorphism with the risk of congenital heart disease in a Chinese population.

    Science.gov (United States)

    Yang, Liping; Gao, Xiaobo; Luo, Haiyan; Huang, Qiuyu; Wei, Ying; Zhang, Guican; Huang, Guoying; Su, Dongmei; Chen, Liangwan; Lu, Cailing; Yang, Juhua; Ma, Xu

    2014-08-01

    MiR-143 plays an important role in the heart development of zebra fish. The rs41291957 variant located in the pri-miR-143 sequence is associated with colorectal carcinogenesis. Therefore, the authors hypothesized that rs41291957 in pri-miR-143 might be involved in the risk of sporadic congenital heart disease (CHD). The authors conducted a case-control study of CHD in a Chinese population to test their hypothesis by genotyping pri-miR-143 rs41291957 in 1,109 CHD cases and 915 non-CHD control subjects. Logistic regression analyses showed no significant association of genotype or allele frequencies of pri-miR-143 rs41291957 A/G polymorphism with the CHD cases in overall or various subtypes compared with the control group. To the authors' knowledge, this is the first study to investigate the relationship between miR-143 and CHD cases. The results demonstrated that rs41291957 in pri-miR-143 has no major role in genetic susceptibility to sporadic CHD, at least in the current study population.

  10. Transcriptional, post-transcriptional and chromatin-associated regulation of pri-miRNAs, pre-miRNAs and moRNAs

    DEFF Research Database (Denmark)

    Nepal, Chirag; Coolen, Marion; Hadzhiev, Yavor;

    2015-01-01

    MicroRNAs (miRNAs) play a major role in the post-transcriptional regulation of target genes, especially in development and differentiation. Our understanding about the transcriptional regulation of miRNA genes is limited by inadequate annotation of primary miRNA (pri-miRNA) transcripts. Here, we...... used CAGE-seq and RNA-seq to provide genome-wide identification of the pri-miRNA core promoter repertoire and its dynamic usage during zebrafish embryogenesis. We assigned pri-miRNA promoters to 152 precursor-miRNAs (pre-miRNAs), the majority of which were supported by promoter associated post....... Developmental profiling revealed a subset of pri-miRNAs that are maternally inherited. Moreover, we show that promoter-associated H3K4me3, H2A.Z and RNAPII marks are not only present at pri-miRNA promoters but are also specifically enriched at pre-miRNAs, suggesting chromatin level regulation of pre...

  11. PRI-CAT: a web-tool for the analysis, storage and visualization of plant ChIP-seq experiments.

    OpenAIRE

    2011-01-01

    Although several tools for the analysis of ChIP-seq data have been published recently, there is a growing demand, in particular in the plant research community, for computational resources with which such data can be processed, analyzed, stored, visualized and integrated within a single, user-friendly environment. To accommodate this demand, we have developed PRI-CAT (Plant Research International ChIP-seq analysis tool), a web-based workflow tool for the management and analysis of ChIP-seq ex...

  12. Approach to Radar Signal Clustering Based on PRI Entropy%基于PRI熵的雷达信号聚类方法研究

    Institute of Scientific and Technical Information of China (English)

    孙盼杰; 刘刚; 王杰

    2008-01-01

    针对脉冲分选聚类中存在的问题,引入熵的概念,定义了 PRI(脉冲重复间隔)熵,给出了雷达脉冲序列之间合并的 PRI 熵判据.并在此基础上提出了新的雷达信号聚类方法.采用先分选后合并的思路,通过计算合并前后 PRI 熵是否减小来判断是否对序列合并.通过仿真验证该方法解决增批问题的有效性.

  13. Transcriptional, post-transcriptional and chromatin-associated regulation of pri-miRNAs, pre-miRNAs and moRNAs.

    Science.gov (United States)

    Nepal, Chirag; Coolen, Marion; Hadzhiev, Yavor; Cussigh, Delphine; Mydel, Piotr; Steen, Vidar M; Carninci, Piero; Andersen, Jesper B; Bally-Cuif, Laure; Müller, Ferenc; Lenhard, Boris

    2016-04-20

    MicroRNAs (miRNAs) play a major role in the post-transcriptional regulation of target genes, especially in development and differentiation. Our understanding about the transcriptional regulation of miRNA genes is limited by inadequate annotation of primary miRNA (pri-miRNA) transcripts. Here, we used CAGE-seq and RNA-seq to provide genome-wide identification of the pri-miRNA core promoter repertoire and its dynamic usage during zebrafish embryogenesis. We assigned pri-miRNA promoters to 152 precursor-miRNAs (pre-miRNAs), the majority of which were supported by promoter associated post-translational histone modifications (H3K4me3, H2A.Z) and RNA polymerase II (RNAPII) occupancy. We validated seven miR-9 pri-miRNAs by in situ hybridization and showed similar expression patterns as mature miR-9. In addition, processing of an alternative intronic promoter of miR-9-5 was validated by 5' RACE PCR. Developmental profiling revealed a subset of pri-miRNAs that are maternally inherited. Moreover, we show that promoter-associated H3K4me3, H2A.Z and RNAPII marks are not only present at pri-miRNA promoters but are also specifically enriched at pre-miRNAs, suggesting chromatin level regulation of pre-miRNAs. Furthermore, we demonstrated that CAGE-seq also detects 3'-end processing of pre-miRNAs on Drosha cleavage site that correlates with miRNA-offset RNAs (moRNAs) production and provides a new tool for detecting Drosha processing events and predicting pre-miRNA processing by a genome-wide assay.

  14. Pri-let-7a-1 rs10739971 polymorphism is associated with gastric cancer prognosis and might affect mature let-7a expression.

    Science.gov (United States)

    Li, Ying; Xu, Qian; Liu, Jingwei; He, Caiyun; Yuan, Quan; Xing, Chengzhong; Yuan, Yuan

    2016-01-01

    The relationship between the pri-let-7a-1 rs10739971 polymorphism and gastric cancer (GC) risk has been reported. However, the role of this polymorphism in the prognosis of GC remains largely elusive. Sequenom MassARRAY platform method and the polymerase chain reaction (PCR)-restriction fragment length polymorphism were used to investigate pri-let-7a-1 rs10739971 G→A in 334 GC patients. Real-time PCR detected expression of mature let-7a in serum and tissue. Patients with AA or GA+AA genotypes of the pri-let-7a-1 rs10739971 polymorphism demonstrated significantly longer survival time than those with the wild GG genotype. Stratified analysis indicated that survival time was significantly longer in women with AA or GA+AA genotypes and in Borrmann type I/II patients with GA heterozygote or GA+AA genotypes. AA genotype was more frequent in the lymphatic-metastasis-negative subgroup. Serum mature let-7a expression in healthy people with the GA heterozygote and the GA+AA genotype was higher than in those with the GG genotype, and the difference remained significant in the female healthy subgroup. Pri-let-7a-1 rs10739971 polymorphism might be a biomarker for GC prognosis, especially for female and Borrmann type I/II patients. The pri-let-7a-1 rs10739971 polymorphism might affect serum mature let-7a expression, and partly explain the mechanism of the relationship between the pri-let-7a-1 rs10739971 polymorphism and GC survival.

  15. A novel method for the modulation type recognition of radar PRI%雷达PRI调制样式识别新方法

    Institute of Scientific and Technical Information of China (English)

    陈晟; 姜秋喜; 潘继飞

    2012-01-01

    Aiming at the shortcoming of the modulation type recognition of radar PRI,five classsified parameters which can effectively describe the modulation characteristics of radar PRI are given based on the analysis of radar PRI types,and the management methods for the missing and spurious pulsesv are analyzed.On these grounds,the recognition arithmetic and realized process for the modulation types of radar PRI are put forword and be used to effectively recognize five representative PRI modulation types.Theoretic analysis and computer simulation indicate that this method is robust in the presence of a low PRI measurement precision and a high rate of missing and spuriou pulses.%针对雷达PRI调制样式识别难题,在分析雷达PRI类型的基础上,提出了能够有效描述雷达PRI调制特征的五种分类特征参数,分析了对存在丢失和虚假脉冲的处理方法,并据此提出了PRI调制样式的识别算法与实现流程,实现了五种典型PRI调制样式的有效识别。理论分析与计算机仿真结果表明,该方法在PRI测量精度较低和丢失虚假脉冲比例较高的情况下具有稳健的识别性能。

  16. Prediction of moment-rotation characteristic of top- and seat-angle bolted connection incorporating prying action

    Science.gov (United States)

    Ahmed, Ali

    2017-02-01

    Finite element (FE) analyses were performed to explore the prying influence on moment-rotation behaviour and to locate yielding zones of top- and seat-angle connections in author's past research studies. The results of those FE analyses with experimental failure strategies of the connections were used to develop failure mechanisms of top- and seat-angle connections in the present study. Then a formulation was developed based on three simple failure mechanisms considering bending and shear deformations, effects of prying action on the top angle and stiffness of the tension bolts to estimate rationally the ultimate moment M u of the connection, which is a vital parameter of the proposed four-parameter power model. Applicability of the proposed formulation is assessed by comparing moment-rotation (M-θ r ) curves and ultimate moment capacities with those measured by experiments and estimated by FE analyses and three-parameter power model. This study shows that proposed formulation and Kishi-Chen's method both achieved close approximation driving M-θ r curves of all given connections except a few cases of Kishi-Chen model, and M u estimated by the proposed formulation is more rational than that predicted by Kishi-Chen's method.

  17. Improved RT-PCR Assay to Quantitate the Pri-, Pre-, and Mature microRNAs with Higher Efficiency and Accuracy.

    Science.gov (United States)

    Tong, Li; Xue, Huihui; Xiong, Li; Xiao, Junhua; Zhou, Yuxun

    2015-10-01

    Understanding of the functional significance of microRNAs (miRNAs) requires efficient and accurate detection method. In this study, we developed an improved miRNAs quantification system based on quantitative real-time polymerase chain reaction (qRT-PCR). This method showed higher efficiency and accuracy to survey the expression of primary miRNAs (pri-miRNAs), precursor miRNAs (pre-miRNAs), and mature miRNAs. Instead of relative quantification method, we quantified the pri-miRNAs and pre-miRNAs with absolute qRT-PCR based on SYBR Green I fluorescence. This improvement corrected for the inaccuracy caused by the differences in amplicon length and PCR efficiency. We also used SYBR Green method to quantify mature miRNAs based on the stem-loop qRT-PCR method. We extended the pairing part of the stem-loop reverse transcript (RT) primer from 6 to 11 bp, which greatly increased the efficiency of reverse transcription PCR (RT-PCR). The performance of the improved RT primer was tested using synthetic mature miRNAs and tissue RNA samples. Results showed that the improved RT primer demonstrated dynamic range of seven orders of magnitude and sensitivity of detection of hundreds of copies of miRNA molecules.

  18. 叶片光化学植被指数(PRI)的修正及其敏感性分析%Sensitivity Study of a Revised Leaf Photochemical Reflectance Index(PRI)

    Institute of Scientific and Technical Information of China (English)

    吴朝阳; 牛铮; 汤泉

    2008-01-01

    光化学植被指数(PRI)被成功的应用于估算不同叶片生化组分的光能利用率(LUE),这些生化组分包括叶片含水量、氮元素浓度等.以往的研究中已经成功建立了叶片、冠层和景观尺度PRI和LUE的相关关系.但是,为了利用PRI更加准确地估算叶片LUE,很多问题仍然亟需解决,比如当LUE高于0.03 molCO2 mol-1PPFD(photosynthetic photon flux density),PRI会出现一个明显的饱和区域以及PRI和LUE关系会随季节发生较大变化.文章对PRI进行了修正,并基于PROSPECT模型对这一修正形式进行了4个参数的敏感性分析.分析结果表明,叶片SR-PRI(Simple ratio PRI)对叶肉结构参数(N)、叶绿素浓度(Cab)敏感性要高于叶片的等效水厚(Cw)和干物质浓度(Cm).因此叶肉结构参数(N)和叶绿素浓度(Cab)的微小变化都会造成SR-PRI的较大变化,进而影响到LUE的估算精度.对SR-PRI的实证实验表明,这一指数也能够很好地用来估算叶片的光能利用率并且结果要优于PRI和LUE之间的相关关系.SR-PRI最重要的一方面在于其更清晰的物理意义和对531 nm处反射率变化的敏感性,因为这一波段位置的反射率变化是评价光能利用率的核心参数.

  19. The Effects of Utilization of PRI/RS on Reading Achievement in the Oklahoma City Public Schools. Evaluation of 1981-82.

    Science.gov (United States)

    Kimball, George H.

    1982-01-01

    A study was conducted to determine (1) the extent to which the degree of use of PRI/RS (the Prescriptive Reading Inventory Reading Systems, a criterion referenced management system developed by CTB/McGraw Hill) affected comparable groups of students on standardized achievement measures; (2) whether the degree of use differentially influenced…

  20. Inhibition of hepatitis B virus replication by helper dependent adenoviral vectors expressing artificial anti-HBV pri-miRs from a liver-specific promoter.

    Science.gov (United States)

    Mowa, Mohube Betty; Crowther, Carol; Ely, Abdullah; Arbuthnot, Patrick

    2014-01-01

    Research on applying RNA interference (RNAi) to counter HBV replication has led to identification of potential therapeutic sequences. However, before clinical application liver-specific expression and efficient delivery of these sequences remain an important objective. We recently reported short-term inhibition of HBV replication in vivo by using helper dependent adenoviral vectors (HD Ads) expressing anti-HBV sequences from a constitutively active cytomegalovirus (CMV) promoter. To develop the use of liver-specific transcription regulatory elements we investigated the utility of the murine transthyretin (MTTR) promoter for expression of anti-HBV primary microRNAs (pri-miRs). HD Ads containing MTTR promoter effected superior expression of anti-HBV pri-miRs in mice compared to HD Ads containing the CMV promoter. MTTR-containing HD Ads resulted in HBV replication knockdown of up to 94% in mice. HD Ads expressing trimeric anti-HBV pri-miRs silenced HBV replication for 5 weeks. We previously showed that the product of the codelivered lacZ gene induces an immune response, and the duration of HBV silencing in vivo is likely to be attenuated by this effect. Nevertheless, expression of anti-HBV pri-miRs from MTTR promoter is well suited to countering HBV replication and development of HD Ads through attenuation of their immunostimulatory effects should advance their clinical utility.

  1. 基于PRI谱的双门限雷达信号分选算法及其硬件平台设计%Double-Threshold PRI Sorting Algorithm Based on PRI Spectrum and Its Sorting Platform Design

    Institute of Scientific and Technical Information of China (English)

    柴娟芳; 司锡才; 马晓东

    2009-01-01

    针对修正的PRI变换法无法分选重频参差雷达信号的缺点,提出了一种基于PRI谱的双门限雷达信号分选算法.该算法先对PRI谱设定双门限检测各个准PRI,再结合组合查看及序列搜索提取各个PRI.同时,构建了一种预分选、主分选结合的新型分选平台.预分选利用带有PowerPC的FPGA构架片上系统,完成对脉冲雷达信号的实时测量、AOA分类及数据存储;主分选采用DSP专职完成其他维参数的分选算法.谊分选新算法在保留抑制PRI子谐波优点的同时,对重频固定、抖动和参差的雷达脉冲信号都有较好的检测结果.视频信号源的大量测试表明,谊分选平台对普通雷达、PRI抖动和参差雷达、频率捷变雷达及一些特殊雷达均具有较好的分选能力.

  2. The interaction effects of pri-let-7a-1 rs10739971 with PGC and ERCC6 gene polymorphisms in gastric cancer and atrophic gastritis.

    Directory of Open Access Journals (Sweden)

    Qian Xu

    Full Text Available BACKGROUND: The aim of this study was to investigate the interaction effects of pri-let-7a-1 rs10739971 with pepsinogen C (PGC and excision repair cross complementing group 6 (ERCC6 gene polymorphisms and its association with the risks of gastric cancer and atrophic gastritis. We hoped to identify miRNA polymorphism or a combination of several polymorphisms that could serve as biomarkers for predicting the risk of gastric cancer and its precancerous diseases. METHODS: Sequenom MassARRAY platform method was used to detect polymorphisms of pri-let-7a-1 rs10739971 G → A, PGC rs4711690 C → G, PGC rs6458238 G → A, PGC rs9471643 G → C, and ERCC6 rs1917799 in 471 gastric cancer patients, 645 atrophic gastritis patients and 717 controls. RESULTS: An interaction effect of pri-let-7a-1 rs10739971 polymorphism with ERCC6 rs1917799 polymorphism was observed for the risk of gastric cancer (P interaction = 0.026; and interaction effects of pri-let-7a-1 rs10739971 polymorphism with PGC rs6458238 polymorphism (P interaction = 0.012 and PGC rs9471643 polymorphism (P interaction = 0.039 were observed for the risk of atrophic gastritis. CONCLUSION: The combination of pri-let-7a-1 rs10739971 polymorphism and ERCC6 and PGC polymorphisms could provide a greater prediction potential than a single polymorphism on its own. Large-scale studies and molecular mechanism research are needed to confirm our findings.

  3. Functional study of one nucleotide mutation in pri-miR-125a coding region which related to recurrent pregnancy loss.

    Science.gov (United States)

    Hu, Yi; Huo, Zheng-Hao; Liu, Chun-Mei; Liu, Shi-Guo; Zhang, Ning; Yin, Kun-Lun; Qi, Lu; Ma, Xu; Xia, Hong-Fei

    2014-01-01

    MicroRNAs (miRNAs) are short non-coding RNAs which modulate gene expression by binding to complementary segments present in the 3'UTR of the mRNAs of protein coding genes. MiRNAs play very important roles in maintaining normal human body physiology conditions, meanwhile, abnormal miRNA expressions have been found related to many human diseases spanning from psychiatric disorders to malignant cancers. Recently, emerging reports have indicated that disturbed miRNAs expression contributed to the pathogenesis of recurrent pregnancy loss (RPL). In this study, we identified a new mutation site (+29A>G, position relative to pre-miR-125a) by scanning pri-miR-125a coding region in 389 Chinese Han RPL patients. This site was co-existed with two polymorphisms (rs12976445 and rs41275794) in patients heterogeneously and changed the predicted secondary structures of pri-miR-125a. Subsequent in vitro analysis indicated that the A>G mutation reduced mature miR-125a expression, and further led to less efficient inhibition of verified target genes. Functional analysis showed that mutant pri-mir-125a can enhance endometrial stromal cells (ESCs) invasive capacity and increase the sensitivity of ESCs cells to mifepristone. Moreover, we further analyzed the possible molecular mechanism by RIP-chip assay and found that mutant pri-mir-125a disturbed the expression of miR-125a targetome, the functions of which includes embryonic development, cell proliferation, migration and invasion. These data suggest that A>G mutation in pri-miR-125a coding region contributes to the genetic predisposition to RPL by disordering the production of miR-125a, which consequently meddled in gene regulatory network between mir-125a and mRNA.

  4. Method of deinterleaving radar signal based on PRI transform algorithm%一种基于PRI变换的雷达信号分选方法

    Institute of Scientific and Technical Information of China (English)

    王海滨; 马琦

    2013-01-01

    With the signal environment of information warfare increasingly complicated, the radar signal sorting technology, as one of development directions of modern radar, is of great importance to radar reconnaissance. Several radar signal deinterleav-ing methods are proposed based on PRI parameter. The traditional PRI transform can overcome the subharmonic problem pro-duced in the histogram statistic methods, but has a poor performance on anti-jitter. The paper begins with a discussion of the im-proved PRI transform which overcomes the disadvantages of traditional PRI method effectively, followed by the description of the algorithm simulation. Finally, a method for sorting pulse repetition intervals of staggered PRI is discussed.%信息作战环境日益复杂,而雷达信号分选技术是作为现代信息对抗领域的重要发展方向之一,对于雷达侦察非常重要.对于雷达信号分选,基于PRI参数提出了很多分选方法.传统的PRI变换能克服直方图统计法中的子谐波问题,但抗抖动性差.讨论了修正的PRI变换分选算法,有效地克服了传统PRI变换的缺点,并对算法进行了计算机仿真.最后还讨论了重频参差抖动脉冲序列的分选方法.

  5. NON-GENICULATE CORALLINE ALGAE (CORALLINALES, RHODOPHYTA FROM THE LOWER OLIGOCENE OF POLJŠICA PRI PODNARTU (NORTHERN SLOVENIA

    Directory of Open Access Journals (Sweden)

    LUKA GALE

    2009-03-01

    Full Text Available Despite their increasing importance in sedimentology and palaeoecology, non-geniculate coralline algae remain virtually overlooked in Slovenia. Though these plants are present or even abundant in the Cretaceous and Cainozoic strata, they have never been studied in detail with notable exception of corallines from the Lower Oligocene beds in the area of Gornji Grad. Poljšica pri Podnartu is another locality where Lower Oligocene beds are exposed, considered as equivalent to the former. The studied profile consists of pebbly limestone, mudstone, sandstone and limestone. Limestones contain abundant non-geniculate coralline algae. Nine species from six genera of these corallines have been identified: Lithoporella melobesioides (Foslie Foslie, 1909, Neogoniolithon contii (Mastrorilli Quaranta et al., 2007, Spongites sp., Lithothamnion sp. 1, Lithothamnion sp. 2, Mesophyllum sp. 1, Mesophyllum sp. 2, Mesophyllum sp. 3 and Sporolithon sp. Some of these species are described from Slovenia for the first time. 

  6. Protein kinase C α regulates nuclear pri-microRNA 15a release as part of endothelin signaling.

    Science.gov (United States)

    von Brandenstein, Melanie; Depping, Reinhard; Schäfer, Ekaterine; Dienes, Hans-Peter; Fries, Jochen W U

    2011-10-01

    Endothelin-1 induced signaling is characterized by an early induction of a nuclear factor-kappa B p65/mitogen-activated phosphokinase p38 transcription complex via its A-receptor versus a late induction via diacylglycerol, and protein kinase C. A possible interaction between these two pathways and a potential function for protein kinase C in this context has not previously been elucidated. Here we report that in Caki-1 tumor cells, protein kinase C α is a part of the transcription complex. With importin α4 and α5 as chaperones, the transcription complex transmigrates into the nucleus. Protein kinase C α blocks the nuclear release of pri-microRNA 15a by direct binding shown by electrophoretic mobility shift assay and Duolink immune histology. The expression levels of miRNA 15a can be further manipulated by transfection of si-protein kinase C α, or an expression vector containing protein kinase C α or miRNA 15. The miRNA 15a regulation by protein kinase C α is detectable in different malignant human tumor cell lines (renal cell carcinoma, breast carcinoma, and melanoma). Furthermore, all three cell lines harbor both endothelin receptors (ETAR/ETBR). Specific blockage of each receptor leads to major reduction of miRNA 15a expression due to increased nuclear protein kinase C α translocation. We conclude that the nuclear binding of pri-microRNA 15a is a novel function of protein kinase C α, which plays an important role in endothelin-1 mediated signaling. Since several endothelin-sensitive, malignant tumor cell lines harbor this regulation, it could indicate a more general role in tumor biology.

  7. Genetic polymorphisms of IL17A and pri-microRNA-938, targeting IL17A 3'-UTR, influence susceptibility to gastric cancer.

    Science.gov (United States)

    Arisawa, Tomiyasu; Tahara, Tomomitsu; Shiroeda, Hisakazu; Matsue, Yasuhito; Minato, Takahiro; Nomura, Tomoe; Yamada, Hideto; Hayashi, Ranji; Saito, Takashi; Matsunaga, Kazuhiro; Fukuyama, Tomoki; Hayashi, Nobuhiko; Otsuka, Toshimi; Fukumura, Atsushi; Nakamura, Masakatsu; Shibata, Tomoyuki

    2012-07-01

    We report an association between gastric cancer (GC) and polymorphisms in IL17A, rs2275913 (-197 G > A), rs3748067 (*1249 C > T), and pri-miR-938, rs2505901 (T > C). We employed the multiplex PCR-SSCP method to detect gene polymorphisms in 337 GC cases and 587 controls. The minor allele frequency of rs2275913 was significantly higher, and those of rs3748067 and rs2505901 significantly lower, in GC cases than controls. The rs2275913 AA homozygote was associated with an increased risk (OR, 2.38; 95%CI, 1.63-3.46; p pri-miR-938 contribute to cancer risk susceptibility and therefore can affect the development of gastric cancer.

  8. Identification and analysis of genetic variations in pri-miRNAs expressed specifically or at a high level in sheep skeletal muscle.

    Directory of Open Access Journals (Sweden)

    Wei Zhang

    Full Text Available MicroRNAs (miRNAs are key regulators in miRNA-mediated gene regulatory networks and play important roles in many biological processes, such as growth and development of mammals. In this study, we used microarrays to detect 261 miRNAs that are expressed in sheep skeletal muscle. We found 22 miRNAs that showed high levels of expression and equated to 89% of the total miRNA. Genetic variations in these 22 pri-miRNAs were further investigated using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP and sequencing. A total of 49 genetic variations, which included 41 single nucleotide polymorphisms (SNPs and 8 deletions/insertions, were identified in four sheep breeds. Three variations were further researched in a larger sample set, including five sheep breeds with different meat production performances. We found that the genotype and allele frequencies of the CCC deletion/insertion in pri-miR-133a were significantly related to the sheep meat production trait. Finally, cell assays and quantitative reverse transcription PCR (qRT-PCR were employed to investigate the effect of pri-miRNA genetic variation on the miRNA biogenesis process. The results confirmed that genetic variations can influence miRNA biogenesis and increase or decrease the levels of mature miRNAs, in accordance with the energy and stability change of hair-pin secondary structures. Our findings will help to further the understanding of the functions of genetic variations in sheep pri-miRNAs in skeletal muscle growth and development.

  9. Prion-like nanofibrils of small molecules (PriSM): A new frontier at the intersection of supramolecular chemistry and cell biology

    OpenAIRE

    Zhou, Jie; Du, Xuewen; Xu, Bing

    2015-01-01

    Formed by non-covalent interactions and not defined at genetic level, the assemblies of small molecules in biology are complicated and less explored. A common morphology of the supramolecular assemblies of small molecules is nanofibrils, which coincidentally resembles the nanofibrils formed by proteins such as prions. So these supramolecular assemblies are termed as prion-like nanofibrils of small molecules (PriSM). Emerging evidence from several unrelated fields over the past decade implies ...

  10. Endocrine and Metabolic Effects of Adipose Tissue in Children and Adolescents / Endokrina in Presnovna Funkcija Maščobnega Tkiva Pri Otrocih in Mladostnikih

    Directory of Open Access Journals (Sweden)

    Kotnik Primož

    2015-03-01

    Full Text Available Maščobno tkivo ima vlogo pri številnih endokrinih in presnovnih procesih. Lepin je bil med prvimi odkritimi dejavniki iz maščobnega tkiva, ki delujejo avto-, para- in endokrino. Od opredelitve leptina so odkrili še številne druge dejavnike, od katerih se nekateri izločajo iz maščobnih celic, nekateri pa iz drugih celic maščobnega tkiva.

  11. 基于PRI的脉冲序列去交错方法%PRI-Based Algorithms for Deinterleaving of Radar Pulses

    Institute of Scientific and Technical Information of China (English)

    周斌

    2002-01-01

    论述使用脉冲重复间隔(PRI)对雷达脉冲去交错的算法,分析PRI变换算法并对算法进行计算机仿真.对PRI变换算法进行改进,并对改进算法进行了计算机仿真和分析.

  12. Pri-miR-34b/c rs4938723 TC heterozygote is associated with increased cancer risks: evidence from published data.

    Science.gov (United States)

    Yi, De-Hui; Wang, Ben-Gang; Zhong, Xin-Ping; Liu, Hao; Liu, Yong-Feng

    2014-12-01

    The promoter region of the microRNA pri-miR-34b/c has a potentially functional polymorphism, rs4938723, located in a typical CpG island. Studies of the association between pri-miR-34b/c rs4938723 polymorphism and risks of various cancers have had inconsistent results. We therefore conducted a meta-analysis of nine studies that included 6,036 cancer patients and 7,490 controls to address this association. Overall, this meta-analysis showed the pri-miR-34b/c rs4938723 TC heterozygote to be significantly associated with increased risk of overall cancers compared with the wild-type TT genotype (P = 0.010, odds ratio (OR) = 1.10, 95 % confidence interval (CI) 1.02-1.18). In stratified analysis, the TC heterozygote was significantly associated with increased cancers risks in digestive tract cancers, in hepatocellular cancer, in Asian population and in the large-sample subgroup. The CC genotypes of rs4938723 were also associated with increased hepatocellular cancer risk but associated with decreased colorectal cancer risk in the stratification analysis by a single cancer type. Thus our meta-analysis suggests that the pri-miR-34b/c rs4938723 TC heterozygote contributes to increased overall cancer risks, as well as shown in digestive tract cancers, in hepatocellular cancer, in Asian population and in the large-sample subgroup. This rs4938723 SNP showed an opposite tendency orientation between the hepatocellular cancer and colorectal cancer risks. Large-sample studies are needed to verify our findings.

  13. Identification and analysis of genetic variations in pri-miRNAs expressed specifically or at a high level in sheep skeletal muscle.

    Science.gov (United States)

    Zhang, Wei; Wang, Limin; Zhou, Ping; Song, Guangchao; Shen, Min; Gan, Shangquan; Shi, Guoqing

    2015-01-01

    MicroRNAs (miRNAs) are key regulators in miRNA-mediated gene regulatory networks and play important roles in many biological processes, such as growth and development of mammals. In this study, we used microarrays to detect 261 miRNAs that are expressed in sheep skeletal muscle. We found 22 miRNAs that showed high levels of expression and equated to 89% of the total miRNA. Genetic variations in these 22 pri-miRNAs were further investigated using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing. A total of 49 genetic variations, which included 41 single nucleotide polymorphisms (SNPs) and 8 deletions/insertions, were identified in four sheep breeds. Three variations were further researched in a larger sample set, including five sheep breeds with different meat production performances. We found that the genotype and allele frequencies of the CCC deletion/insertion in pri-miR-133a were significantly related to the sheep meat production trait. Finally, cell assays and quantitative reverse transcription PCR (qRT-PCR) were employed to investigate the effect of pri-miRNA genetic variation on the miRNA biogenesis process. The results confirmed that genetic variations can influence miRNA biogenesis and increase or decrease the levels of mature miRNAs, in accordance with the energy and stability change of hair-pin secondary structures. Our findings will help to further the understanding of the functions of genetic variations in sheep pri-miRNAs in skeletal muscle growth and development.

  14. Single-nucleotide polymorphism of the pri-miR-34b/c gene is not associated with susceptibility to congenital heart disease in the Han Chinese population.

    Science.gov (United States)

    Liu, Y-M; Wang, Y; Peng, W; Wu, Z; Wang, X-H; Wang, M-L; Wang, W; Sun, J; Zhang, Z-D; Mo, X-M

    2013-08-12

    Recent evidence has shown that the microRNA polymorphism may play an important role in the susceptibility to congenital heart disease (CHD). A potentially functional SNP rs4938723 (T>C) in the promoter region of pri-miR-34b/c might affect transcription factor GATA binding and therefore pri-miR-34b/c expression. We genotyped the pri-miR-34b/c polymorphism in a case-control study of 590 patients and 672 controls in a Han Chinese population and assessed the effects of the pri-miR-34b/c polymorphism on CHD susceptibility by TaqMan SNP genotyping assay. There was no association between the pri-miR-34b/c polymorphism and the risk of CHD in both genotype and allelic frequency. In a subsequent analysis of the association between this polymorphism and CHD classification, there was still no significant difference in both genotype and allelic frequency. Our results suggest that the pri-miR-34b/c polymorphism rs4938723 is not associated with susceptibility to sporadic CHD in the Han Chinese population.

  15. Zeaxanthin-independent energy quenching and alternative electron sinks cause a decoupling of the relationship between the photochemical reflectance index (PRI) and photosynthesis in an evergreen conifer during spring.

    Science.gov (United States)

    Fréchette, Emmanuelle; Wong, Christopher Y S; Junker, Laura Verena; Chang, Christine Yao-Yun; Ensminger, Ingo

    2015-12-01

    In evergreen conifers, the winter down-regulation of photosynthesis and its recovery during spring are the result of a reorganization of the chloroplast and adjustments of energy-quenching mechanisms. These phenological changes may remain undetected by remote sensing, as conifers retain green foliage during periods of photosynthetic down-regulation. The aim was to assess if the timing of the spring recovery of photosynthesis and energy-quenching characteristics are accurately monitored by the photochemical reflectance index (PRI) in the evergreen conifer Pinus strobus. The recovery of photosynthesis was studied using chlorophyll fluorescence, leaf gas exchange, leaf spectral reflectance, and photosynthetic pigment measurements. To assess if climate change might affect the recovery of photosynthesis, seedlings were exposed to cold spring conditions or warm spring conditions with elevated temperature. An early spring decoupling of the relationship between photosynthesis and PRI in both treatments was observed. This was caused by differences between the timing of the recovery of photosynthesis and the timing of carotenoid and chlorophyll pool size adjustments which are the main factors controlling PRI during spring. It was also demonstrated that zeaxanthin-independent NPQ mechanisms undetected by PRI further contributed to the early spring decoupling of the PRI-LUE relationship. An important mechanism undetected by PRI seems to involve increased electron transport around photosystem I, which was a significant energy sink during the entire spring transition, particularly in needles exposed to a combination of high light and cold temperatures.

  16. Application of carbon nanosorbent for PRiME pass-through cleanup of 10 selected local anesthetic drugs in human plasma samples.

    Science.gov (United States)

    Ma, Jian-Bo; Zhao, Li-Ming; Rui, Qiu-Hong; Liao, Yu-Feng; Chen, Yan-Min; Xu, Jin; Zhu, Yan; Zhao, Yong-Gang

    2017-04-01

    A novel PRiME (process, robustness, improvements, matrix effects, ease of use) pass-through cleanup procedure has been developed to improve the existing commercially available designs. Carbon nanosorbents, i.e., magnetic modified carboxyl-graphene (Mag-CG) and magnetic modified carboxyl-carbon nanotubes (Mag-CCNTs), have been synthesised and evaluated in PRiME pass-through cleanup procedure for human plasma prior to analysis of 10 selected local anesthetic drugs by liquid chromatography-tandem quadrupole mass spectrometry (LC-MS/MS). The matrix effect, an interesting phenomenon of ion suppression for local anesthetic drugs containing ester group and ion enhancement for other drugs containing acylamino group, has been minimized using carbon nanosorbents PRiME pass-through cleanup procedure. Under the optimal conditions, the obtained results show higher cleanup efficiency of the carbon nanosorbents with recoveries between 70.2% and 126%. Furthermore, the carbon nanosorbents are also evaluated for reuse up to 80-100 times. The limits of quantification (LOQs) for local anesthetic drugs are in the range of 0.024-0.15 μg/L. Validation results on linearity, specificity, accuracy, and precision, as well as the application to the analysis of lidocaine in five patients recruited from the lung cancer demonstrate the applicability to clinical studies.

  17. The impact of pri-miR-218 rs11134527 on the risk and prognosis of patients with esophageal squamous cell carcinoma.

    Science.gov (United States)

    Jiang, Lin; Wang, Chaofu; Sun, Canlin; Xu, Yumin; Ding, Zhongqi; Zhang, Xueling; Huang, Junxing; Yu, Hong

    2014-01-01

    MicroRNA-218 (miR-218) acts as a tumor suppressor in numerous types of cancer by regulation of the expression of target genes. The aim of this study was to investigate whether polymorphisms in miR-218 LAMB3 pathway were associated with the risk and prognosis of esophageal squamous cell carcinoma (ESCC). Pri-mir-218 rs11134527 and LAMB3 rs2566 were genotyped in ESCC patients and 745 controls to assess their associations with cancer risk and overall survival. Pri-mir-218 rs11134527 was significantly associated with a decreased risk of ESCC under codominant, recessive and additive models. Although there was a significant association between rs11134527 and better survival of ESCC patients under codominant, recessive and additive models, the association disappeared after adjustment for TNM and LNM. However, further stratified analysis revealed that the association remained significant in patients with TNM stages I and II or non-LNM. Our data suggest that pri-miR-218 rs11134527 may contribute to the genetic susceptibility and prognosis for ESCC in Chinese Han population.

  18. Magnetic and electric behavior of praseodymium substituted CuPryFe2-yO4 ferrites

    Science.gov (United States)

    Farid, Muhammad Tahir; Ahmad, Ishtiaq; Kanwal, Muddassara; Murtaza, Ghulam; Ali, Irshad; Ashiq, Muhammad Naeem; Khan, Sajjad Ahmad

    2017-01-01

    Spinel ferrites with chemical formula CuPryFe2-yO4(y=0.0,0.02,0.04,0.06,0.08,0.10) were synthesized using sol-gel technique. The sintered powder characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) and vibrating sample magnetometer (VSM). X-ray Diffraction analysis showed that the samples with concentration (y=0.0-0.06) have single spinel phase and the samples with concentration at y=0.08-0.10 have secondary phase (ortho ferrite phase) along spinel phase. The Lattice constant a increased with the substitution of Praseodymium (Pr) ions. The crystallite size calculated from Scherre's formula is found in the range of 53-37 nm. It is observed that DC resistivity and activation energy enhances with the substitution of Pr. The dc resistivity decreased with the increase in temperature. The conduction below Curie temperature (Tc) is due to hopping of electrons and holes, whereas, above Curie temperature it is due to polaron hopping. The decrease in Tc with increasing Pr constant may be due to the fact that Pr-Fe interactions on the B sites are weaker than Fe-Fe interaction. The saturation magnetization (Ms) and remanence (Mr) decreased where as coercivity (Hc) has been increased with the increase of Pr content. The measured parameters suggest that these materials are potential candidates for high frequency applications.

  19. Pri-miR-34b/c rs4938723 polymorphism contributes to acute lymphoblastic leukemia susceptibility in Chinese children.

    Science.gov (United States)

    Tong, Na; Chu, Haiyan; Wang, Meilin; Xue, Yao; Du, Mulong; Lu, Lingling; Zhang, Heng; Wang, Feng; Fang, Yongjun; Li, Jie; Wu, Dongmei; Zhang, Zhengdong; Sheng, Xiaojing

    2016-01-01

    A polymorphism rs4938723 (T > C) within the promoter region of pri-miR-34b/c has been found to not only affect the expression of mature miR-34b/c but also contribute to the susceptibility to several cancer types. We designed a case-control study to evaluate the role of rs4938723 in childhood acute lymphoblastic leukemia (ALL). The rs4938723 CC genotype was significantly associated with reduced ALL risk (p = 0.003, ORadjusted = 0.51, 95% CI = 0.33-0.80 for CC vs. TT). Stratification analyses showed the differences were pronounced in older (> 6 years), male subjects, as well as in patients in low risk and T-ALL subtypes. The in vitro luciferase assays in Jurkat and K-562 cell lines showed that the transcription activity of miR-34b/c was increased when T allele transited to C allele (p < 0.05). In conclusion, rs4938723 genetic variant contributed to the susceptibility to Chinese childhood ALL by influencing the transcription activity of miR-34b/c promoter.

  20. A New Algorithm of PRI Transform%一种新的PRI变换分选算法

    Institute of Scientific and Technical Information of China (English)

    李英达; 肖立志; 于洋; 康一丁; 王宇

    2014-01-01

    脉冲重复间隔(pulse repetition interval,PRI)变换及其改进算法是雷达信号分选领域的经典算法之一.在早期的信号环境中,各部雷达信号的PRI值相对接近,传统的改进PRI变换算法能够有效地分选PRI抖动雷达信号,因而得到了广泛的应用.随着信号环境的日益复杂,不同雷达发射的信号其PRI值分布在一个较大的范围内,此时传统算法的分选能力大大减弱.针对上述问题,对传统算法中可变时间起点的改变、PRI箱的选择以及门限的确定三个方面进行改进,提出一种新的PRI变换分选算法.新算法中采用了可变的移动因子、固定的箱分辨率以及合理的门限,在保留原算法优点的同时,能够适应PRI值分布在较大范围内的抖动雷达信号分选问题,并通过模拟仿真验证了算法的有效性.

  1. Characterization of Staphylococcus aureus Primosomal DnaD Protein: Highly Conserved C-Terminal Region Is Crucial for ssDNA and PriA Helicase Binding but Not for DnaA Protein-Binding and Self-Tetramerization.

    Directory of Open Access Journals (Sweden)

    Yen-Hua Huang

    Full Text Available The role of DnaD in the recruitment of replicative helicase has been identified. However, knowledge of the DNA, PriA, and DnaA binding mechanism of this protein for the DnaA- and PriA-directed replication primosome assemblies is limited. We characterized the DNA-binding properties of DnaD from Staphylococcus aureus (SaDnaD and analyzed its interactions with SaPriA and SaDnaA. The gel filtration chromatography analysis of purified SaDnaD and its deletion mutant proteins (SaDnaD1-195, SaDnaD1-200 and SaDnaD1-204 showed a stable tetramer in solution. This finding indicates that the C-terminal region aa 196-228 is not crucial for SaDnaD oligomerization. SaDnaD forms distinct complexes with ssDNA of different lengths. In fluorescence titrations, SaDnaD bound to ssDNA with a binding-site size of approximately 32 nt. A stable complex of SaDnaD1-195, SaDnaD1-200, and SaDnaD1-204 with ssDNA dT40 was undetectable, indicating that the C-terminal region of SaDnaD (particularly aa 205-228 is crucial for ssDNA binding. The SPR results revealed that SaDnaD1-195 can interact with SaDnaA but not with SaPriA, which may indicate that DnaD has different binding sites for PriA and DnaA. Both SaDnaD and SaDnaDY176A mutant proteins, but not SaDnaD1-195, can significantly stimulate the ATPase activity of SaPriA. Hence, the stimulation effect mainly resulted from direct contact within the protein-protein interaction, not via the DNA-protein interaction. Kinetic studies revealed that the SaDnaD-SaPriA interaction increases the Vmax of the SaPriA ATPase fivefold without significantly affecting the Km. These results indicate that the conserved C-terminal region is crucial for ssDNA and PriA helicase binding, but not for DnaA protein-binding and self-tetramerization.

  2. pri-miR-21重组腺病毒载体构建及其对TLR4基因调控的研究%Construction of a recombined adenovirus vector carrying pri-miR-21 gene and research on it's target gene TLR4

    Institute of Scientific and Technical Information of China (English)

    赵婧; 徐广贤; 贾伟; 董辉; 张一琳; 赵志军; 魏军

    2012-01-01

    目的:构建能高效表达成熟miR-21小分子的腺病毒载体,探讨其对TLR4基因的靶向调控关系.方法:以正常小鼠基因组DNA为模板,PCR扩增pri-miR-21基因,克隆至穿梭载体pAdTrack-CMV中经PCR、酶切及基因测序分析正确无误后,与pAdEasy-1腺病毒骨架质粒进行同源重组,并利用293A细胞,包装成pri-miR-21重组腺病毒感染HeLa细胞,通过Westem blot检测TLR4的蛋白表达水平,验证miR-21与TLR4靶向调控的关系.结果:经PCR、酶切、测序及GFP表达证实,成功构建携带pri-miR-21基因的重组腺病毒载体并制备出高滴度重组病毒.Western blot证实,miR-21可抑制TLR4蛋白的表达.结论:所制备的小鼠pri- miR-21重组腺病毒,可以高效表达成熟miR-21小分子,能够抑制靶基因TLR4的表达.%AIM: To construct the recombined adenovi-rus vector carrying pri-miR-21 gene, which can express mature miR-21 efficiently, and to study the interaction of miR-21 with its target gene TLR4. METHODS: Using healthy mouse's gDNA as template, the primary miR-21 coding sequence was amplified by PCR and cloned into a shuttle vector pAdTrack-CMV. Constructed plasmid was sequenced and linearized for homologous recombination with pAdEasy-1 vector in BJ5183 bacteria. The recombined adenovirus vector carrying pri-miR-21 gene was used to challenge HeLa cell. The candidate target gene of miR-21 was determined by miRNA analysis databases. The expression level of TLR4 protein was detected by western blotting. RESULTS; Through the PCR, restriction enzyme digestion, DNA sequencing and expression of GFP, recombinant adenoviral vector pri-miR-21 gene was constructed successfully. Bioin-formatic analysis suggested a few possible binding sites between miR-21 and TLR4. Results showed that miR-21 down-regulated TLR4 at protein levels. CONCLUSION-. The recombinant adenoviral vector containing pri- miR-21 was successfully constructed. miR-21 gene interfered with the expression of TLR4 target gene.

  3. Improvement of the design and generation of highly specific plant knockdown lines using primary synthetic microRNAs (pri-smiRNAs

    Directory of Open Access Journals (Sweden)

    Alves Leonardo

    2010-03-01

    Full Text Available Abstract Background microRNAs (miRNAs are endogenous small non-coding RNAs that post-transcriptionally regulate gene expression. In plants, they typically show high complementarity to a single sequence motif within their target mRNAs and act by catalyzing specific mRNA cleavage and degradation. miRNAs are processed from much longer primary transcripts via precursor miRNAs containing fold-back structures. Leaving these secondary structures intact, miRNAs can be re-designed experimentally to target mRNAs of choice. Results We designed primary synthetic miRNAs (pri-smiRNAs on the basis of the primary transcript of the Arabidopsis MIR159A gene by replacing the original miR159a and the corresponding miR159a* with novel sequences, keeping the overall secondary structure as predicted by the program RNAfold. We used the program RNAhybrid to optimize smiRNA design and to screen the complete Arabidopsis transcriptome for potential off-targets. To improve the molecular cloning of the pri-smiRNA we inserted restriction sites in the original MIR159A primary transcript to easily accommodate the smiRNA/smiRNA* DNA fragment. As a proof-of-concept, we targeted the single gene encoding chalcone synthase (CHS in Arabidopsis. We demonstrate smiRNA(CHS expression and CHS mRNA cleavage in different transgenic lines. Phenotypic changes in these lines were observed for seed color and flavonol derivatives, and quantified with respect to anthocyanin content. We also tested the effect of mismatches and excess G:U base pairs on knockdown efficiency. Conclusions RNAhybrid-assisted design of smiRNAs and generation of pri-smiRNAs using a novel vector containing restriction sites greatly improves specificity and speed of the generation of stable knockdown lines for functional analyses in plants.

  4. Thermochemical cycles for the heat and cold long-range transport. Final report of the PRI 9.2 Cold transport. Annual report of the PR 2-8; Cycles thermochimiques pour le transport de chaleur et de froid a longue distance. Rapport final du PRI 9.2. Transport de froid. Rapport annuel du PR 2-8

    Energy Technology Data Exchange (ETDEWEB)

    Luo, L.; Tondeur, D. [Laboratoire des Sciences du Genie Chimique (LSGC), 54 - Nancy (France); Mazet, N.; Neveu, P.; Stitou, D.; Spinner, B. [Institut de Science et de Genie des Materiaux et Procedes (IMP), 66 - Perpignan (France)

    2004-07-01

    This PRI deals with the use of thermochemical processes, based on solid-gas reversible transformation, to transfer heat of cold at long-range distance (> 10 km), in order to enhance the energy efficiency. Four main aspects have been studied to confirm the process feasibility: the process identification and the operating conditions, the selection of compatible reagents, the design of an auto-thermal reactor and the gas transport impact on the global performances. (A.L.B.)

  5. Multilevel hierarchy of phase separation processes in La5/8-yPryCa3/8MnO3

    Science.gov (United States)

    García-Muñoz, J. L.; Collado, A.; Aranda, M. A. G.; Ritter, C.

    2011-07-01

    We investigate two successive hierarchized phase segregations in La5/8-yPryCa3/8MnO3, which generate ferromagnetic metallic volumes (V1FM and V2FM) having different physical origins, stabilities, and parent electronic phases. The singular percolative transition around TC2 ≈ 100 K is due to a second electronic phase separation (PS), which is specific to the charge-order insulating regions segregated at the first PS process. These findings shed new light on the metastability, atypical dynamics, the nonvolatile magnetoresistance, or the supercooling glass transition in this complex magnetic system.

  6. UČINAK KALIJEVOG I NATRIJEVOG LAKTATA NA ODRŽIVOST JUNEĆEG MLJEVENOG MESA PRI RAZLIČITIM UVJETIMA SKLADIŠTENJA

    OpenAIRE

    Šmidt, Gordana; Kovačić, Mirela; Grgurić, Ivana; Bertinovec, Ines

    2009-01-01

    Svježe mljeveno juneće meso tretirano kalijevim laktatom (KL) i natrijevim laktatom (NaL) u količini od 4% dodanima pojedinačno, odnosno svaki po 2% kada su kombinirani, upakirano je u modificiranu atmosferu sačinjenu od O2 i CO2 u omjeru 80%:20%, i skladišteno pri 2ºC i 8ºC kroz 7 dana. Uzorci su analizirani prvi, četvrti, šesti i sedmi dan. Rezultati ispitivanja pokazali su da je utjecaj KL i NaL na broj aerobnih mezofilnih bakterija podjednak, te su tretirani uzorci...

  7. Combined analysis of pri-miR-34b/c rs4938723 and TP53 Arg72Pro with cervical cancer risk.

    Science.gov (United States)

    Yuan, Fang; Sun, Ruifen; Chen, Peng; Liang, Yundan; Ni, Shanshan; Quan, Yi; Huang, Juan; Zhang, Lin; Gao, Linbo

    2016-05-01

    miR-34 family members can form a p53-miR-34 positive feedback loop and induce apoptosis, DNA repair, angiogenesis, and cell cycle arrest. We conducted a case-control study to examine whether two polymorphisms (i.e., rs4938723 in the promoter of pri-miR-34b/c and TP53 Arg72Pro) were linked to the carcinogenesis of cervical cancer among Chinese Han women. Genotypes of the two polymorphisms in 328 cervical cancer patients and 568 control subjects were determined by using a polymerase chain reaction-restriction fragment length polymorphism assay. We found a significantly increased cervical cancer risk in the pri-miR-34b/c rs4938723 under dominant and overdominant model (CT/CC vs. TT: adjusted OR = 1.34, 95 % CI = 1.01-1.77; CT vs. TT/CC: adjusted OR = 1.37, 95 % CI = 1.05-1.80, respectively). Increased cervical cancer risks were also found in the TP53 Arg72Pro under a heterozygous comparison and overdominant model (CG vs. GG: adjusted OR = 1.44, 95 % CI = 1.06-1.95; CG vs. GG/CC: adjusted OR = 1.47, 95 % CI = 1.12-1.94, respectively). Stratification analysis showed that patients carrying the pri-miR-34b/c rs4938723 CT genotype had a significantly increased risk for developing poorly differential status and clinical stage I. Moreover, increased cancer risks were observed for the TP53 Arg72Pro polymorphism in patients with poorly differential status, clinical stage II, and without lymph node metastasis. Combined analysis revealed that the genotypes of rs4938723 CT/CC and TP53 Arg72Pro CG/CC had an increased cervical cancer risk (OR = 2.21, 95 % CI = 1.38-3.53). These findings suggest that the pri-miR-34b/c rs4938723 and TP53 Arg72Pro polymorphisms may contribute to the genesis of cervical cancer.

  8. Quantitative analysis of histidine decarboxylase gene (hdcA) transcription and histamine production by Streptococcus thermophilus PRI60 under conditions relevant to cheese making.

    Science.gov (United States)

    Rossi, Franca; Gardini, Fausto; Rizzotti, Lucia; La Gioia, Federica; Tabanelli, Giulia; Torriani, Sandra

    2011-04-01

    This study evaluated the influence of parameters relevant for cheese making on histamine formation by Streptococcus thermophilus. Strains possessing a histidine decarboxylase (hdcA) gene represented 6% of the dairy isolates screened. The most histaminogenic, S. thermophilus PRI60, exhibited in skim milk a high basal level of expression of hdcA, upregulation in the presence of free histidine and salt, and repression after thermization. HdcA activity persisted in cell extracts, indicating that histamine might accumulate after cell lysis in cheese.

  9. Quantitative Analysis of Histidine Decarboxylase Gene (hdcA) Transcription and Histamine Production by Streptococcus thermophilus PRI60 under Conditions Relevant to Cheese Making▿†

    Science.gov (United States)

    Rossi, Franca; Gardini, Fausto; Rizzotti, Lucia; La Gioia, Federica; Tabanelli, Giulia; Torriani, Sandra

    2011-01-01

    This study evaluated the influence of parameters relevant for cheese making on histamine formation by Streptococcus thermophilus. Strains possessing a histidine decarboxylase (hdcA) gene represented 6% of the dairy isolates screened. The most histaminogenic, S. thermophilus PRI60, exhibited in skim milk a high basal level of expression of hdcA, upregulation in the presence of free histidine and salt, and repression after thermization. HdcA activity persisted in cell extracts, indicating that histamine might accumulate after cell lysis in cheese. PMID:21378060

  10. The genetic association between pri-miR-34b/c polymorphism (rs4938723 T > C) and susceptibility to cancers: evidence from published studies.

    Science.gov (United States)

    Li, Xiaowei; Wang, Liguang; Yu, Jianyu; Xu, Jun; Du, Jiajun

    2014-12-01

    Recently, several molecular epidemiological studies have focused on the association between pri-miR-34b/c rs4938723 SNP and the susceptibility to different cancers. Due to the controversial rather than conclusive results, we performed this meta-analysis to assess more precise and comprehensive conclusion about the association. Data published until July 2014 were collected from PubMed, Embase, Web of Science, Chinese National Knowledge Infrastructure, Wanfang Data, Chinese BioMedical Literature Database, and VIP database of Chinese Journal. Ultimately, 13 articles with a total of 7,753 cases and 8,014 controls were considered eligible for inclusion. The odds ratio (OR) and its 95 % confidence interval (95%CI) were used to assess the strength of association. In the overall analysis, a significant association between pri-miR-34b/c rs4938723 polymorphism and increased cancer susceptibility was found in heterozygous model (TC vs. TT: OR = 1.148, 95%CI 1.034-1.275, P = 0.010) and dominant model (CC + TC vs. TT: OR =1.166, 95%CI 1.028-1.322, P = 0.017). In subgroup analysis of ethnicity, pri-miR-34b/c rs4938723 polymorphism was significantly associated with an increased cancer susceptibility for Asian population in heterozygous model (TC vs. TT: OR = 1.169, 95%CI 1.031-1.326, P = 0.015) and dominant model (CC + TC vs. TT: OR = 1.185, 95%CI 1.017-1.382, P = 0.030), whereas no significant association for Caucasian population was observed in any genetic models. Intriguingly, stratified analysis revealed opposite results that pri-miR-34b/c polymorphism contributed to susceptibility to hepatocellular carcinoma while reduced susceptibility to colorectal cancer and esophageal squamous cell cancer in Asians. Considering some limitation of our meta-analysis, future well-designed case-control studies with larger sample sizes are required to confirm our findings.

  11. Deinterleaving of Radar Signals Based on Modified PRI Transform Algorithm%一种改进型PRI变换雷达信号分选技术

    Institute of Scientific and Technical Information of China (English)

    赵永胜

    2007-01-01

    在对传统的脉冲重复间隔(PRI)变换法进行介绍的基础上,提出了一种改进型的PRI变换法来估计脉冲重复间隔,并给出了相应的门限设定方法,另外,文中还提出了一种适用的快速脉冲抽取算法.仿真表明,该方法对固定PRI、抖动PRI和参差PRI脉冲序列都有较好的估计效果.

  12. Optimizacija svojstava samoorganiziranja graft kopolimera metoksi poli(etilen glikola) i masnih kiselina pri pripravi nanonosača s uklopljenim etopozidom

    OpenAIRE

    Varshosaz, Jaleh; HASANZADEH, FARSHID; ESLAMDOOST, MOJTABA

    2012-01-01

    Cilj je rada bio ispitati svojstva samoorganiziranja micela pripravljenih od graft kopolimera metoksipolietilenglikola (mPEG) i masnih kiselina različite duljine lanca za uklapanje etopozida. Sintetizirana su tri amfifilna kopolimera povezivanjem miristinske, stearinske i behenične kiseline esterskom vezom s mPEG. Veličina i zeta-potencijal micela određeni su metodom dinamičkog raspršivanja svjetlosti. Micele s uklopljenim etopozidom pripravljene su film-metodom pri različitim omjerima lijeka...

  13. Conductivity of single-crystal Y1-yPryBa2Cu3O7-δ over a wide range of temperatures and Pr concentrations

    Science.gov (United States)

    Khadzhai, G. Ya.; Vovk, N. R.; Vovk, R. V.

    2014-06-01

    The in-plane electrical resistance of Y1-yPryBa2Cu3O7-δ single crystals with a high degree of perfection is studied for temperatures in the range TC-300 K, where TC ranges from 92 to 52 K, with varying praseodymium content. The experimental data are approximated by an expression that takes electron scattering on phonons and defects into account, as well as fluctuation conductivity in a 3D Aslamazov-Larkin model. According to the approximation data the Debye temperature ranges from 350-370 K, depending on the praseodymium content. The transverse coherence length is ˜1 Å.

  14. La democracia interna en los partidos políticos: el caso del PRI de México entre 1965-1984

    OpenAIRE

    Contreras Romero, Valente Alberto

    2015-01-01

    El presente trabajo de investigación pretende analizar los procesos de selección interna de candidatos a puestos de elección popular dentro de los partidos políticos especialmente aquellos donde se apliquen métodos de selección incluyentes y democraticos. El trabajo se enfocara principalmente en los partidos políticos hegemónicos en regímenes poco democraticos como fue el caso de México, para este efecto se realizará un estudio de caso aplicado al Partido Revolucionario Institucional (PRI) ...

  15. Congés… pour « prières » Prayers and days off

    Directory of Open Access Journals (Sweden)

    Claudine Viard

    2009-04-01

    Full Text Available Les premiers combats de la classe ouvrière se sont portés sur la demande d’une journée de repos, qui par tradition chrétienne a été fixée le dimanche en 1906. Quatre journées de congé à caractère chrétien ont en outre été retenues par le législateur. L’exercice du culte est donc concrètement garanti pour les chrétiens. Avec l’arrivée sur le territoire de la République  de populations de confessions variées, cette organisation du travail apparaît inégalitaire, voire de nature à porter atteinte à la liberté religieuse. Il a été proposé de reconnaître les jours de prières d’autres confessions. Pour l’instant, le législateur n’a pas adopté ces propositions. Mais la question reste posée. Or, la jurisprudence des tribunaux judiciaires et administratifs admet le droit de prier des fidèles des cultes minoritaires, ils peuvent prétendre à un congé lors des fêtes de leur religion dans la mesure où cela n’occasionne pas de perturbation. La consécration de cette jurisprudence souple par le législateur serait de nature à calmer le débat.At the turn of the century, the working classes fought for a day off in the week, which was granted on Sundays by the legislator in 1906. Four additional christian holidays are also legal. So the right to pray is garanteed for christians. As different populations with various faiths settled in France, this organisation has seemed to violate the principle of equality, and religious freedom. There has been a debate on the question whether some of the days of minority religions should also be observed by all the population. But so far, the legislator has not voted any of these proposals. It remains an issue. The French judge has consistently decided that the people of non christian faiths had the right to be granted a day off to pray on these special days, provided it would not cause prejudice to the organisation they work for. It might help to calm the debate if this

  16. Pri-miR-34b/c rs4938723 polymorphism is associated with a decreased risk of gastric cancer.

    Science.gov (United States)

    Pan, Xin-Min; Sun, Rui-Fen; Li, Zhao-Hui; Guo, Xiao-Min; Qin, Hao-Jie; Gao, Lin-Bo

    2015-04-01

    Previous studies have demonstrated that miR-34 family members are abnormally expressed in gastric cancer. Overexpression of the miR-34 family suppresses gastric carcinogenesis, whereas downregulation of the miR-34 family promotes tumorigenesis. p53 can bind to the promoter region of miR-34b/c, leading to an increase of miR-34b/c expression. Recently, a variant in the promoter region of pri-miR-34b/c (rs4938723) has been discovered, with the function of altering the binding efficiency of transcription factor GATA. The purpose of this study was to examine the role of the miR-34b/c rs4938723 and TP53 Arg72Pro polymorphisms in the susceptibility of gastric cancer. We analyzed the distribution of the two polymorphisms in 197 patients with gastric cancer and 289 age-, gender-, ethnicity-, and living area-matched controls using polymerase chain reaction-restriction fragment length polymorphism and DNA direct sequencing. We found that the CT and CT/CC genotypes of the miR-34b/c rs4938723 were associated with a significantly decreased risk of gastric cancer compared with the TT genotype (CT vs. TT: odds ratio [OR]=0.66; 95% confidence interval [95% CI], 0.45-0.97; and CT/CC vs. TT: OR=0.67; 95% CI, 0.47-0.97, respectively). Combined analysis showed that subjects carrying the miR-34b/c rs4938723 CT/CC and TP53 CG/CC genotypes had a 0.62-fold decreased risk to develop gastric cancer compared with subjects carrying the miR-34b/c rs4938723 TT and TP53 CG/CC genotypes (OR=0.62; 95% CI, 0.40-0.96). These findings suggest that the miR-34b/c rs4938723 may individually and jointly have a protective effect on the risk of gastric risk.

  17. PAZ, PRI, AND PROGRESS: OCTAVIO PAZ’S POLITICAL, ECONOMIC AND LITERARY STRUGGLE TO INSPIRE REFORM IN TWENTIETH-CENTURY MEXICO

    Directory of Open Access Journals (Sweden)

    D. Gene Pace

    2003-01-01

    Full Text Available Throughout most of the twentieth century; Mexico’s political and economic policies were heavily influenced by the Partido Revolucionario Institutional (PRI. The landmark 1998 victory by Vicente Fox, the first presidential candidate in seven decades to win without the official sanction of the PRI,marked an important milestone in Mexican history; Octavio Paz, an eloquent proponent of political, economic, and social reform, sought for decades to inspire change. This paper seeks to illuminate Paz’s economic philosophy, and to demonstrate how the acclaimed writer, through courageous symbolic action coupled with an inimitable and potent pen, challenged the PRI’s hegemony in Mexico and contributed to the historic election he almost lived to celebrate (an elderly Paz died shortly before the historic 1998 election.“The Aztec ritual of 2 October [1968] in the Plaza de Tlatelolco. . . convinced me to abandon the Mexican Foreign Service.”“October 2, 1968 ended the student movement. It also ended an era in thehistory of Mexico.”

  18. PRI变换在脉冲列去交错中的适用性分析%Applicability Analysis of PRI Transform in Pulse Train Destagger

    Institute of Scientific and Technical Information of China (English)

    安振; 李运祯

    2007-01-01

    传统雷达脉冲列去交错方法中的脉冲重复间隔(PRI)估计算法,是以接收到的脉冲串的自相关函数为基础,很容易出现信号的PRI及其整数倍(即子谐波)同时存在的现象,给雷达脉冲串的去交错造成了困难.为克服上述缺点,提出了PRI变换的估计方法,几乎完全抑制了出现在自相关函数中的子谐波.研究了这种算法对于各种不同PRI信号的适用性.计算机仿真结果表明,这种算法适用于检测不同信号的PRI.

  19. Nova libro pri interlingvistiko

    Index Scriptorium Estoniae

    2006-01-01

    Rets rmt.: Blanke, Detlev. Interlinguistische Beiträge : zum Wesen und zur Plansprachen / herausgegeben von Sabine Fiedler. Frankfurt/Main, Berlin ; Bern ; Bruxelles ; New York ; Oxford ; Wien : Europäischer Verlag der Wissenschaften Peter Lang

  20. Patología del sueño en las enfermedades priónicas Sleep disorders in prion diseases

    Directory of Open Access Journals (Sweden)

    T. Ayuso

    2007-01-01

    Full Text Available Las enfermedades priónicas son un grupo de encefalopatías con cambios neurodegenerativos causados por una proteína alterada denominada prión cuyo dato característico es la transmisibilidad. Ocurren la mayoría de las veces de forma esporádica aunque un grupo de ellas son familiares asociadas a mutaciones en el gen de la proteína priónica. El polimorfismo genético parece determinar las diferentes variantes familiares. Una de las más enigmáticas e inhabituales es el Insomnio Letal Familiar (ILF, trastorno hereditario caracterizado por pérdida del sueño fisiológico con estupor onírico, hiperactividad autonómica y motora, y anomalías motoras. La polisomnografía de esta entidad refleja la incapacidad para producir un patrón fisiológico del sueño NREM y REM, así como de las fluctuaciones circadianas hormonales y vegetativas; la transición de vigilia a sueño está marcadamente alterada con desaparición precoz de los husos de sueño. La hipótesis del origen de estos trastornos es la pérdida neuronal talámica, especialmente en los núcleos anterior y dorsomedial, descrita en la neuropatología de estos pacientes; además la PET revela hipofunción de núcleos talámicos, centros responsables del control vigilia-sueño. En la enfermedad de Creutzfeldt-Jakob las alteraciones de sueño-vigilia no se han considerado características, no obstante, se han encontrado frecuentes alteraciones en los registros electroencefalográficos de sueño. Además de la neurodegeneración talámica puede haber mecanismos etiopatogénicos comunes en las enfermedades priónicas en relación con la función biológica de la proteína priónica.Prion diseases are a group of encephalopathies with neurodegenerative changes caused by an altered protein named prion whose characteristic datum is transmissibility. In most cases they occur in a sporadic form although a group of them are familial associated with mutations in the gene of the prion protein

  1. 一种基于PRI谱特征的复杂雷达信号分选方法%A method of complex radar signal sorting based on PRI spectrum signatures

    Institute of Scientific and Technical Information of China (English)

    王亚莉; 胡进

    2011-01-01

    通过分析雷达截获机所处的复杂雷达信号环境,提出了一种基于PR I谱特征的复杂雷达信号分选方法。该方法利用PR I变换获取雷达信号PR I谱,并根据各种复杂雷达信号PR I谱特征实现复杂雷达信号的分选工作。仿真结果表明,该方法能够较好地实现复杂信号环境下雷达信号的分选工作。%In view of the complex radar signal environment for radar interceptors,a method of the complex radar signal sorting based on the PRI spectrum signatures is presented.The PRI spectrum is obtained through the PRI transform,and the complex radar signal sorting is realized based on the PRI spectrum signatures of various complex radar signals.The simulation results show that the method can better realize radar signal sorting in the complex signal environment.

  2. Base-pair opening dynamics of the microRNA precursor pri-miR156a affect temperature-responsive flowering in Arabidopsis.

    Science.gov (United States)

    Kim, Hee-Eun; Kim, Wanhui; Lee, Ae-Ree; Jin, Suhyun; Jun, A Rim; Kim, Nak-Kyoon; Lee, Joon-Hwa; Ahn, Ji Hoon

    2017-03-18

    Internal and environmental cues, including ambient temperature changes, regulate the timing of flowering in plants. Arabidopsis miR156 represses flowering and plays an important role in the regulation of temperature-responsive flowering. However, the molecular basis of miR156 processing at lower temperatures remains largely unknown. Here, we performed nuclear magnetic resonance studies to investigate the base-pair opening dynamics of model RNAs at 16 °C and investigated the in vivo effects of the mutant RNAs on temperature-responsive flowering. The A9C and A10CG mutations in the B5 bulge of the lower stem of pri-miR156a stabilized the C15∙G98 and U16∙A97 base-pairs at the cleavage site of pri-miR156a at 16 °C. Consistent with this, production of mature miR156 was severely affected in plants overexpressing the A9C and A10CG constructs and these plants exhibited almost no delay in flowering at 16 °C. The A10G and A9AC mutations did not strongly affect C15∙G98 and U16∙A97 base-pairs at 16 °C, and plants overexpressing A10G and A9AC mutants of miR156 produced more mature miR156 than plants overexpressing the A9C and A10CG mutants and showed a strong delay in flowering at 16 °C. Interestingly, the A9AC mutation had distinct effects on the opening dynamics of the C15∙G98 and U16∙A97 base-pairs between 16 °C and 23 °C, and plants expressing the A9AC mutant miR156 showed only a moderate delay in flowering at 16 °C. Based on these results, we propose that fine-tuning of the base-pair stability at the cleavage site is essential for efficient processing of pri-miR156a at a low temperature and for reduced flowering sensitivity to ambient temperature changes.

  3. Sun-induced Chlorophyll fluorescence and PRI improve remote sensing GPP estimates under varying nutrient availability in a typical Mediterranean savanna ecosystem

    Science.gov (United States)

    Perez-Priego, O.; Guan, J.; Rossini, M.; Fava, F.; Wutzler, T.; Moreno, G.; Carvalhais, N.; Carrara, A.; Kolle, O.; Julitta, T.; Schrumpf, M.; Reichstein, M.; Migliavacca, M.

    2015-07-01

    This study investigates the performances of different optical indices to estimate gross primary production (GPP) of herbaceous stratum in a Mediterranean savanna with different Nitrogen (N) and Phosphorous (P) availability. Sun-induced chlorophyll Fluorescence yield computed at 760 nm (Fy760), scaled-photochemical reflectance index (sPRI), MERIS terrestrial-chlorophyll index (MTCI) and Normalized difference vegetation index (NDVI) were computed from near-surface field spectroscopy measurements collected using high spectral resolution spectrometers covering the visible near-infrared regions. GPP was measured using canopy-chambers on the same locations sampled by the spectrometers. We hypothesized that light-use efficiency (LUE) models driven by remote sensing quantities (RSM) can better track changes in GPP caused by nutrient supplies compared to those driven exclusively by meteorological data (MM). Particularly, we compared the performances of different RSM formulations - relying on the use of Fy760 or sPRI as proxy for LUE and NDVI or MTCI as fraction of absorbed photosynthetically active radiation (fAPAR) - with those of classical MM. Results showed significantly higher GPP in the N fertilized experimental plots during the growing period. These differences in GPP disappeared in the drying period when senescence effects masked out potential differences due to plant N content. Consequently, although MTCI was tightly related to plant N content (r2 = 0.86, p < 0.01), it was poorly related to GPP (r2 = 0.45, p < 0.05). On the contrary sPRI and Fy760 correlated well with GPP during the whole measurement period. Results revealed that the relationship between GPP and Fy760 is not unique across treatments but it is affected by N availability. Results from a cross validation analysis showed that MM (AICcv = 127, MEcv = 0.879) outperformed RSM (AICcv = 140, MEcv = 0.8737) when soil moisture was used to constrain the seasonal dynamic of LUE. However, residual analyses

  4. A Recognition Algorithm for Radar PRI Modulation Mode Based on Extremum Sequence Features Set%基于极值序列特征集的雷达PRI调制模式识别算法

    Institute of Scientific and Technical Information of China (English)

    周一鹏; 王星; 田元荣; 周东青; 程嗣怡

    2016-01-01

    识别雷达信号的脉冲重复间隔( PRI)调制模式是分析雷达工作状态和工作任务的重要手段。针对复杂体制雷达的PRI调制模式可实时切换并改变调制参数因而难于识别的问题,提出一种基于极值序列特征集的雷达PRI调制模式识别算法。该算法首先提取PRI序列的极值特征,构建极值序列特征集;然后,基于PRI序列及其特征集建立恒参、类正弦、正弦和抖动判定准则,实现雷达PRI调制模式的分层识别。仿真分析表明:该算法对复杂体制雷达PRI调制模式的识别正确率达95.3%,同时具有较高的实时性,在电子对抗应用领域具有良好的前景。%The recognition of pulse repetition interval ( PRI) modulation mode is meaningful for analyzing the condition and task of radar.In order to recognize advanced radar which could change its PRI modulation mode and parameters quickly , a recognition al-gorithm based on extremum sequence features set is proposed .Firstly, by extracting the extremum sequence features from PRI se-quence , the PRI extremum features set is constructed .Then the judge criterions of five PRI modulation modes based on PRI se-quence and its extremum features set is proposed .Finally, a multi-layer recognition algorithm is presented .The result shows that the recognition algorithm has preferable recognition correct rate (95.3%), and could classify the PRI modulation modes quickly , so the resae rch has a good application prospect in electronic support measures.

  5. 一种抗 SDIF 分选的脉冲重复间隔参差设计方法%A Design Method of PRI Stagger Countering the SDIF Sorting Algorithm

    Institute of Scientific and Technical Information of China (English)

    张保群

    2016-01-01

    To improve the anti-sorting and anti-recognition ability of radars against electronic reconnaissance systems,a design method of PRI stagger for radar signals was proposed based on the idea of destroying the performance of sorting with the pulse repetition interval (PRI).The working process of a typical PRI sorting algorithm,the sequential difference histogram (SDIF)was analyzed,and some critical steps were discussed.It is revealed that,when the staggered PRI values are chosen properly under some contracts,the correct sorting processing can be destroyed,as a result,more than one targets appear in the sorting results.Simulation results show that,the presented design is effective during countering the signal sorting based on the SDIF,therefore it provides a preliminary theoretical guidance for the radio frequency stealth design of radar signals.%为提高雷达对电子侦察系统抗分选识别能力,从破坏脉冲重复间隔(PRI)分选性能的角度出发,提出了一种雷达信号 PRI 参差设计方法。根据典型的序列差值直方图(SDIF)算法的工作流程,分析了 SDIF 实现 PRI 参差信号分选的关键步骤。合理设计参差 PRI 取值,使其满足一定约束条件,可以破坏正确分选流程,达到分选结果中目标增批的目的。仿真结果表明,该设计方法能有效对抗基于 SDIF 的信号分选策略,可以为雷达信号射频隐身设计提供初步的理论指导。

  6. PRI 2.1: Basic researches on the photovoltaic: new materials (july 2002 - july 2004). Final report of the integrated research project; PRI 2.1: Recherches de base en photovoltaique: nouveaux materiaux (Juillet 2002 - Juin 2004). Rapport final du projet de recherche integre

    Energy Technology Data Exchange (ETDEWEB)

    Marfaing, Y.

    2004-07-01

    The main material of the photovoltaic cells is the massive crystalline silicon. For a large scale development of the photovoltaic industry, it is necessary to use thin layers. Many materials are possible to reach this objective: different types of silicon (amorphous, mono-crystal, poly-crystal), poly-crystal of CIS type (CuInSe{sub 2}) and organic molecules and polymers. The last two types are innovative and need some researches. These research axis are presented in this PRI. (A.L.B.)

  7. PRI Detection of Segment Pulse Trains Based on Transform Spectrum Coherent Accumulation%基于变换谱相干积累的片段信号PRI检测

    Institute of Scientific and Technical Information of China (English)

    张什永; 胡泽宾; 王俊文

    2012-01-01

    Due to the effects of search modes and targets' beam scheduling, the reconnaissance systems can only intercept segment pulse trains from emitters, which is a big challenge for signal sorting. For this problem, a new method based on PRI transform spectrum coherent accumulation is proposed. PRI transform algorithm is introduced. The relationship between PRI spectrum peak value and pulse number included is given and its coherent accumulation feature is then revealed. Simulation results show that the new method can efficiently enhance PRI spectrum and is favourable to improve the detection propability.%受自身搜索方式和目标辐射源波束调度的影响,侦察系统往往只能截获辐射源的部分信号,这为后续分选处理带来了很大挑战.针对该问题,提出了通过时PRI变换谱进行相干积累以提高真实PRI检测概率的方法.首先介绍了PRI变换法,对算法原理进行了说明;接着从理论上推导了PRI变换谱峰值与脉冲个数的关系,指出了PRI变换谱的相干积累特性,并分析了其对虚假脉冲的适应能力;最后通过仿真试验对理论分析进行了验证.仿真结果表明,文中提出的方法能够有效提高片段信号的PRI 谱峰,从而有利于提高检测概率.

  8. An accurate estimation algorithm for PRI based on remainder of the cycle%一种基于余数周期的 PRI精确估计算法

    Institute of Scientific and Technical Information of China (English)

    苏焕程; 张君; 程良平; 程亦涵; 冷魁

    2016-01-01

    针对传统脉冲重复间隔(PRI)分选算法在估计PRI方面存在的不足,提出了一种对PRI周期信号的周期进行精确估计的算法。该算法首先从待分选脉冲序列中提取出属于一部雷达的脉冲样本,然后利用同余方程的余数周期性质对该雷达脉冲序列的PRI进行精确的估计。相对于传统的PRI估计算法,该算法有效地消除了TOA量化误差对PRI估计造成的影响,可以精确地估计出雷达脉冲序列的准确 PRI数值,从而能够更好地满足信号分选算法的处理需求。理论推导及仿真实验均表明了该算法的有效性。%For the defects in estimating pulse repetition interval (PRI) of traditional PRI de‐interleaving al‐gorithms ,a new algorithm of estimating the period of PRI periodic signals is put forward .The algorithm firstly extracts the pulse sample sequence of certain radar from the raw pulse sequence ,and then executes precise period estimation process based on the remainder of the cycle .Compared with the traditional PRI estimating algorithms ,this algorithm accurately estimates the PRI with TOA quantization error removing , which satisfies the requirement of the de‐interleaving process . Both theoretical derivation and simulation results verify the validity of the proposed algorithm .

  9. Izobraževalna debata kot metoda makro-didaktične komponente preverjanje z ocenjevanjem pri pouku KEMIJE in NARAVOSLOVJA v osnovni šoli

    OpenAIRE

    Horvat, Davorin

    2014-01-01

    Hiter napredek znanosti in informacijske tehnologije ter dostopnosti do informacij preko medmrežja silijo k drugačnemu, alternativnemu načinu poučevanja (Pisar, 2006). Učence je potrebno uvajati v interdisciplinarno povezovanje aktualnih predmetnih področij. Aktivno učenje s pomočjo različnih metod dela ima pri vzgojno–izobraževalnem procesu čedalje večji pomen. Z metodo izobraževalne debate pridobijo učenci nekatere veščine, potrebne za uspešno soočanje z izzivi današnjega časa. Ta metod...

  10. Extracción de una Proteasa Anti-Priónica de Líquenes (Orden: LECANORALES) en Fluido Gástrico e Intestinal Artificial -resumen-

    OpenAIRE

    Ricardo Millán-González; J Ducett; Johnson, C.

    2014-01-01

    La proteína priónica anormal (PrPTSE) es el agente etiológico de las encefalopatías espongiformes trasmisibles. Esta proteína posee una alta resistencia a ser inactivada por agentes químicos y ambientales‚ como la radiación y el calor‚ lo cual le da la capacidad de persistir en la tierra durante un periodo mayor a tres años después de haber sido enterrado‚ generando así una reserva de infectividad. Los líquenes son organismos simbióticos conformados por un hongo y un agente fotosintético que ...

  11. La proteína priónica celular: Análisis de su función neuroprotectora y reguladora del ciclo Celular

    OpenAIRE

    Carulla Martí, Patricia

    2013-01-01

    [spa] Las encefalopatías espongiformes transmisibles (EETs) son un conjunto de enfermedades neurodegenerativas letales que afectan tanto animales como humanos. Están causadas por unas partículas proteicas denominadas “priones”, resultantes de la transformación de la proteína priónica celular (PrP(C)) a una forma patogénica denominada PrP(SC), que se acumula en el cerebro en forma de agregados e induce la transformación de más moléculas a expensas de la reducción de los niveles de PrP(C)...

  12. Lower Oligocene non-geniculate coralline red algal (Corallinales, Rhodophyta assemblage from Poljšica pri Podnartu (Upper Carniola, Slovenia

    Directory of Open Access Journals (Sweden)

    Luka Gale

    2008-12-01

    Full Text Available The Lower Oligocene Gornji Grad beds from Polj{ica pri Podnartu consist of marly limestone, mudstone, several layers of limestones and two layers of sandstones, and were deposited on a mixed carbonate-siliciclastic ramp.Especially the limestones contain rich fossil fauna and non-geniculate coralline red algae. These were systematicallycollected from four horizons and researched in thin sections under an optical microscope. Genera Lithoporella,Neogoniolithon, Spongites, Lithothamnion, Mesophyllum and Spongites were recognized. Surface area for each genus was calculated and the differences in the coralline assemblages in the four horizons were analysed. Thecorallines originate from two source areas: sandy-muddy bottom of a shallow marine environment, and small coral bioherms with its encrusters.

  13. Fazna ravnoteža u sustavu NaNO3/KNO3 + glicerol + voda pri 288.15 K i 298.15 K

    OpenAIRE

    Zhang, H.-Y.; Zhu, W.-W.; Qin, G.-L.; Wei, Z.

    2014-01-01

    U ternarnom sustav NaNO3/KNO3 + glicerol + voda određeni su topljivost, gustoća i indeks loma pri 288,15 K i 298,15 K. Topljivost je izračunata na temelju gustoće i indeksa loma. U svim se slučajevima topljivost smanjuje s povećanjem udjela glicerola. Za indeks loma uočen je suprotan trend. Dodatkom glicerola smanjuje se gustoća otopine NaNO3, a povećava gustoća otopine KNO3. Eksperimentalni podatci za zasićene i podzasićene otopine korelirani su empirijskom jednadžbom s četiri i sedam parame...

  14. 基于PRI的雷达脉冲序列分选方法%Algorithms for Sorting of Radar Pulses Based on PRI

    Institute of Scientific and Technical Information of China (English)

    杨文华; 高梅国

    2005-01-01

    高密度复杂信号环境下的信号分选处理技术是新一代雷达截获接收机信号处理的关键技术,也是雷达截获领域一个亟待解决的问题.文中讨论基于脉冲重复间隔(PRI)的脉冲分选算法,深入分析了基于直方图统计算法和PRI变换算法的优缺点,并对算法进行计算机仿真.最后针对这两种算法的优缺点提出一种综合的分选算法.

  15. 基于PRI变换和小波变换的雷达信号分选%Sorting of Radar Signal Based on PRI transfer and wavelet transfer

    Institute of Scientific and Technical Information of China (English)

    韩俊; 何明浩; 翟卫俊; 陈昌孝

    2007-01-01

    分析了脉冲重复间隔(PRI)变换算法和小波变换算法的基本原理,针对两种算法在雷达信号分选中的优缺点,提出了一种基于PRI变换和小波变换相结合的雷达信号综合分选方法.该方法首先利用PRI变换对雷达信号粗分选,然后应用小波变换进行细分选.仿真结果表明,在信噪比不低于10dB的条件下,该方法准确可行.

  16. Odpadna embalaža in problem pri odpadni embalaži s pretisnimi omoti v farmacevtskem podjetju Krka d.d.

    OpenAIRE

    Krajnc, Urška

    2015-01-01

    Diplomska naloga obsega problem, ki nastane zaradi čedalje večjega in obsežnejšega nastajanja odpadne embalaže, in konkreten problem, s katerim se srečujejo v farmacevtskem podjetju Krka, d. d., iz Novega mesta. Kot nam je znano, je z odpadki vse več težav. S prostorskega vidika je to kopičenje odpadkov, z okoljskega vidika pa onesnaževanje okolja. Embalaža, ki predstavlja odpadek, ki ga ne potrebujemo več, nam predstavlja predmet, ki ga odvržemo. Pri tem je smiselno premisliti, če je embalaž...

  17. A SNP site in pri-miR-124 changes mature miR-124 expression but no contribution to Alzheimer's disease in a Mongolian population.

    Science.gov (United States)

    Qi, Lu; Hu, Yi; Zhan, Ying; Wang, Jing; Wang, Bing-Bing; Xia, Hong-Fei; Ma, Xu

    2012-04-25

    Increasing evidence shows that single nucleotide polymorphisms (SNPs) or mutations in microRNAs (miRNAs) sequence may affect the processing and function of miRNAs and participate in the occurrence of diseases. Although many SNPs of miRNAs were found, their functions in the pathological process of nerve cells were only just emerging. In the present study, the effect of the SNP of one neuronal miRNA, miR-124, on miRNA biogenesis and human genetic disease was investigated using in vitro cell line model and Alzheimer's disease (AD) in the Mongolian population. Bioinformatics prediction showed that a common G/C polymorphism designated rs531564 was found in the pri-miR-124 and the G allele changed the formation of a ring-shaped structure in the predicted secondary structure of the pri-miRNA for miR-124-1. Northern blot and real-time PCR analysis showed that the amount of mature miR-124 from the C/G heterozygosity of rs531564 was increased compared with the CC or GG homozygosity of rs531564. The expression of mature miR-124 from GG homozygosity was also higher than that from CC homozygosity. But in an association study of AD patients and controls, neither genotype nor allele distribution difference was found in AD patients compared with controls. Collectively, the present study is the first to evaluate the relationship between miR-124 and AD in the Mongolian population. SNP rs531564 of miR-124 may not represent a risk factor in the development of AD among Mongolian population.

  18. A novel marsupial pri-miRNA transcript has a putative role in gamete maintenance and defines a vertebrate miRNA cluster paralogous to the miR-15a/miR-16-1 cluster.

    Science.gov (United States)

    Au, Phil Chi Khang; Frankenberg, Stephen; Selwood, Lynne; Familari, Mary

    2011-10-01

    Successful maintenance, survival and maturation of gametes rely on bidirectional communication between the gamete and its supporting cells. Before puberty, factors from the gamete and its supporting cells are necessary for spermatogonial stem cell and primordial follicle oocyte maintenance. Following gametogenesis, gametes rely on factors and nutrients secreted by cells of the reproductive tracts, the epididymis and/or oviduct, to complete maturation. Despite extensive studies on female and male reproduction, many of the molecular mechanisms of germ cell maintenance remain relatively unknown, particularly in marsupial species. We present the first study and characterisation of a novel primary miRNA transcript, pri-miR-16c, in the marsupial, the stripe-faced dunnart. Bioinformatic analysis showed that its predicted processed miRNA - miR-16c - is present in a wide range of vertebrates, but not eutherians. In situ hybridisation revealed dunnart pri-miR-16c expression in day 4 (primordial germ cells) and day 7 (oogonia) pouch young, in primary oocytes and follicle cells of primordial follicles but then only in follicle cells of primary, secondary and antral follicles in adult ovaries. In the adult testis, pri-miR-16c transcripts were present in the cytoplasm of spermatogonial cells. The oviduct and the epididymis both showed expression, but not any other somatic tissues examined or conceptuses during early embryonic development. This pattern of expression suggests that pri-miR-16c function may be associated with gamete maintenance, possibly through mechanisms involving RNA transfer, until the zygote enters the uterus at the pronuclear stage.

  19. 利用光化学反射植被指数估算光能利用率研究的进展%The Review of Estimating Light Use Efficiency through Photochemical Reflectance Index(PRI)

    Institute of Scientific and Technical Information of China (English)

    陈晋; 唐艳鸿; 陈学泓; 杨伟

    2008-01-01

    植被光能利用率(LUE)是估计植被初级生产力(GPP)和净初级生产力(NPP)模型的一个重要输入,准确地估计LUE对于生态学研究有重要的意义.由于LUE随环境的变化关系十分复杂,现有的LUE估算模型过于粗糙简单,而通过遥感直接估计LUE将会更加可靠.研究表明,光化学反射植被指数(PRI)与LUE有很好的相关性,故PRI在利用遥感估计LUE方面具有极大的潜力.但是很多研究也发现了PRI-LUE的关系不够稳健,受许多因素的干扰,比如物种构成、冠层结构及大气等.因此,PRI的广泛应用还需要更多的研究.本文首先介绍了PRI的定义及PRI随LUE变化的生理机制,再综述了一些利用遥感手段建立PRI-LUE关系的例子,然后分析了影响PRI-LUE关系的各种干扰因素,最后对PRI研究取得的成果、存在的问题以及发展前景作了总结.

  20. A study on signal sorting algorithm based on PRI spectrum signatures for staggered radars%基于PRI谱内特征的参差雷达信号分选算法研究

    Institute of Scientific and Technical Information of China (English)

    韩卫国; 胡进; 倪静静

    2011-01-01

    In view of the basic signatures of radar signals and some deficiencies of the PRI transform algorithm in the signal sorting,a signal sorting algorithm based on the PRI spectrum signatures for staggered radars is presented.The time-domain PRI spectrum of t%通过分析参差雷达信号的基本特征,针对PRI变换算法在参差雷达信号分选中存在的不足,提出了一种基于PRI谱内特征的参差雷达信号分选算法。该算法利用PRI变换获取信号的时域PRI谱,进而根据PRI谱内脉冲对数特征完成参差信号帧周期的分选工作。仿真结果表明,该方法能够较好地实现参差雷达信号的分选工作。

  1. Comparison of the architectural and urban design workshop: in the town of Križevci pri Ljutomeru and the Jelovica architectural workshop

    Directory of Open Access Journals (Sweden)

    Tomaž Krušec

    2013-07-01

    Full Text Available The aim of the paper is to draw attention to the key advantages and potential disadvantages of the different types of architectural workshops and architectural/urban design workshops, i.e. based on an analysis and comparison of two workshops: architectural and urban design workshop in the town of Križevci pri Ljutomeru and the architectural workshop organised by the Jelovica Hiše d.o.o. company. The first workshop tried to find practical solutions to certain spatial problems of the town that the workshop addressed, while the client of the second workshop expected from the students to develop innovative solutions in using a specific building system for designing contemporary residential typologies. The differences in expectations meant that the workshops were carried out differently. In the first workshop, it was essential that the students stayed in the town that was the subject of the workshop, while in the second one, it was important that the students grasped the characteristics of the technological process and the special features of the building. To achieve this, the students made several visits to the company’s production plants and, as an end product of the workshop, the students had to produce a part of their designed structure. This was a most direct way of familiarising themselves with the way of building

  2. A SNP in pri-miR-10a is associated with recurrent spontaneous abortion in a Han-Chinese population.

    Science.gov (United States)

    Li, Ying; Wang, Xue-Qin; Zhang, Lu; Lv, Xiao-Dan; Su, Xing; Tian, Shi; Liu, Chun-Mei; Ma, Xu; Xia, Hong-Fei

    2016-02-16

    MicroRNA-10a (miR-10a) has a wide range of functions in nearly all mammalian tissues and is involved in the occurrence of many diseases. However, it remains unknown whether miR-10a is associated with human recurrent spontaneous abortion (RSA). In this study, we found that rs3809783 A > T in miR-10a coding region was significantly associated with the increase of the risk of human unexplained RSA (URSA) acquisition in a Han-Chinese population. The T allele of rs3809783 hindered the production of mature miR-10a. A to T substitution in miR-10a rs3809783 repressed cell proliferation and migratory capacity. Further investigation discovered that Bcl-2-interacting mediator (Bim) was the functional target of miR-10a and inversely regulated Bim expression. Dual-luciferase assay indicated that A allele in miR-10a rs3809783 could more effectively suppress Bim expression than T allele. In addition, A to T substitution in miR-10a rs3809783 attenuated the sensibility of cells to progesterone and its antagonist mifepristone. Collectively, our data suggest that rs3809783 A > T in pri-miR-10a may be conductive to the genetic predisposition to RSA by disrupting the production of mature miR-10a and reinforcing the expression of Bim.

  3. microTSS: accurate microRNA transcription start site identification reveals a significant number of divergent pri-miRNAs.

    Science.gov (United States)

    Georgakilas, Georgios; Vlachos, Ioannis S; Paraskevopoulou, Maria D; Yang, Peter; Zhang, Yuhong; Economides, Aris N; Hatzigeorgiou, Artemis G

    2014-12-10

    A large fraction of microRNAs (miRNAs) are derived from intergenic non-coding loci and the identification of their promoters remains 'elusive'. Here, we present microTSS, a machine-learning algorithm that provides highly accurate, single-nucleotide resolution predictions for intergenic miRNA transcription start sites (TSSs). MicroTSS integrates high-resolution RNA-sequencing data with active transcription marks derived from chromatin immunoprecipitation and DNase-sequencing to enable the characterization of tissue-specific promoters. MicroTSS is validated with a specifically designed Drosha-null/conditional-null mouse model, generated using the conditional by inversion (COIN) methodology. Analyses of global run-on sequencing data revealed numerous pri-miRNAs in human and mouse either originating from divergent transcription at promoters of active genes or partially overlapping with annotated long non-coding RNAs. MicroTSS is readily applicable to any cell or tissue samples and constitutes the missing part towards integrating the regulation of miRNA transcription into the modelling of tissue-specific regulatory networks.

  4. 基于PRI变换的雷达信号分选%De-interleaving of Radar Signals Based on PRI Transform Algorithm

    Institute of Scientific and Technical Information of China (English)

    邹顺; 张群飞; 靳学明

    2006-01-01

    雷达信号分选是电子对抗的关键技术,而脉冲重复间隔是雷达信号分选的关键参数.目前基于PRI参数提出了很多分选方法.传统的PRI变换法对脉冲重复间隔具有很高的估计精度,它克服了直方图统计法中的子谐波问题,但是它的抗抖动能力很差.该文引入的修正的PRI变换法针对传统PRI变换法的缺点,利用可变的时间起点避免了相位因子的相位差,利用重叠的PRI箱增加PRI箱宽度,有效地克服了传统PRI变换中的缺点.此外,文中还给出了相应的门限设定方法和快速的脉冲抽取算法.仿真表明,该修正的PRI变换算法对固定PRI、抖动PRI和参差PRI脉冲序列都有较好的估计效果,它是精度较高的雷达信号分选方法.

  5. The exploitation of micromorphological parameters for identification in the section / Mentha Využitie mikromorfologických parametrov pri identifikácii v sekcii Mentha

    Directory of Open Access Journals (Sweden)

    Fialová S.

    2015-06-01

    Full Text Available Identifikacia druhov v rode Mentha L. je obzvlašť naročna. Je to sposobene ľahkym križenim podporenym gynodioeciou, ktora je ďalej skomplikovana polymorfizmom, pestovanim, polyploiditou a vegetativnym rozmnožovanim, čo vysvetľuje genetickopopulačne a mikroevolučne pozadie premenlivosti v rode Mentha. V tejto praci sme študovali využitie mikromorfologickych a fytochemickych parametrov pri identifikacii a charakterizacii vybranych druhov rodu Mentha. Na spodnej pokožke listov 20 populacii Mentha sme stanovili veľkosť a počet „žliazok typu Lamiaceae“. Silicu sme analyzovali pomocou GC MS. Identifikovali a charakterizovali sme populacie druhov M. × piperita, M. spicata, M. spicata subsp. condensata, M. spicata var. crispa, M. spicata var. citrata, M. × gentilis, M. aquatic, M. arvensis a M. longifolia. Sledovanie veľkosti a počtu „žliazok typu Lamiaceae prispievaju k charakterizacii niektorych druhov Mentha (najma M. longifolia a M. × piperita.

  6. Li tter Fall i n A Pri mary an d Two Logged -over Lowland Tropical Rainfore sts in Pasirmayang, Jam bi.

    Directory of Open Access Journals (Sweden)

    UPIK ROSALI NA WAS R IN

    1999-01-01

    Full Text Available Litte r accu mulatio n in a prim ary and a log g ed-ove r lo wland dipterocarp fore st at Pa si rmayang, Ja mbi wa s measu red u sin g the litte r trap met hod . In the p rimary fore st , tra p s were placed in fou r d istinc t areas, reflectin g th e su ccess ion stages of the fo rest fr om building t o mat u ration. In the logged-over fo re st , litter p roduc tion w a s measured at two differe nt si tes, one cut in 197 9/1980 a nd t h e second i n 1 983/1984. In the prim ary fore st , ave rage litter production during the ob serv ation period wa s 925 g m"2yr '. In the lo gged -over f o r e st , ave rage l itte r production wa s 721 g m '2 yr1 for t h e sit e cut in 1979 /198 0 and 70 6 g m '2 yr1 f o r the site cut in 19 83/1 984 . Leave s com p ri sed the major con tri butor of litter wit h 67% of tota l litter produced in t h e pri m ary forest, 67% of total litter i n the 1979/ 1980 cut log g ed-o ver fore st, and 65 % of total li tter in the 1983/1984 cut logged-over fore st . The pu rp ose of the stu dy wa s to use litte r fa ll a s a measure of forest pro ductivi ty to a ssess the recovery of log g ed -over forests and , t o pro v ide a basi s for co mpariso n o f forest-de rived land practices for app ropriate fo re st manage ment stra tegies .

  7. Polymorphisms of the pri-miR-34b/c promoter and TP53 codon 72 are associated with risk of colorectal cancer.

    Science.gov (United States)

    Oh, Jisu; Kim, Jong Woo; Lee, Bo Eun; Jang, Moon Ju; Chong, So Young; Park, Pil Won; Hwang, Seong Gyu; Oh, Doyeun; Kim, Nam Keun

    2014-02-01

    The microRNA (miR)-34 family is a direct transcriptional target of tumor-suppressor TP53 and loss of miR-34 function may impair TP53-mediated cell cycle arrest and apoptosis. In the present study, we investigated whether the single nucleotide polymorphisms (SNPs) rs4938723 (T>C) in the promoter region of miR-34b/c and Arg72Pro (G>C) in codon 72 of TP53 are independently or complementarily associated with the risks and clinical outcomes of colorectal cancer (CRC) and whether the combined effect of these SNPs and metabolic risk factors are related to CRC. We evaluated the SNPs in 545 CRC patients and 428 healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequence analysis. We found that the GC and GC/CC genotypes of TP53 Arg72Pro were associated with decreased risk of CRC (adjusted OR = 0.727 for GC; OR = 0.735 for GC/CC). The combined genotypes of TT-GC and CC-GG were significantly associated with reduced CRC risk (adjusted OR = 0.628 for TT-GC; OR = 0.381 for CC-GG, respectively). The SNP rs4938723 and diabetes mellitus (DM) together were associated with an increased CRC risk, but the SNP TP53 Arg72Pro CC with DM showed a protective effect against CRC. These findings indicate that rs4938723 in the promoter region of pri-miR-34b/c and the SNP in TP53 codon 72 were related to decreased risk of CRC in the population studied and those metabolic diseases and genetic variants influence each other with regard to CRC susceptibility.

  8. A Radar Emitter Recognition Method Based on the PRI Switching Rules%一种基于PRI变化规律的雷达辐射源识别方法

    Institute of Scientific and Technical Information of China (English)

    关一夫; 张国毅; 王晓峰

    2015-01-01

    针对相控阵雷达中某些具有复杂PRI调制样式的工作模式难以准确识别的问题,提出一种通过提取PRI序列变化规律来进行辐射源识别的方法。该方法借鉴了脉冲样本图的模型以及生物信息学中基因序列比对的动态规划思想,较好地解决了某些复杂PRI调制样式不好描述,以及相控阵雷达中一个完整工作模式包含脉冲序列长度太短,以致很难通过统计的方法对其进行准确识别的问题。仿真结果表明该算法的有效性,而且在存在脉冲丢失和干扰脉冲的情况下具有稳健性。%Focusing on the problem that phased-array radar has some modes with complex PRI modulation patterns are difficult to accurately identify,this paper proposes a recognition method by extracting the switching rules of the PRI sequence. This method draws on the pulse template model and the dynamic programming theory of bioinformatics in gene sequence alignment,having solved the difficulty in describing some complex PRI modulation pattern and those for statistical methods in recognizing phased-array radars with mode includes a complete sequence of pulses with very short length. Simulation results show the effectiveness of the algorithm and its robustness even in the presence of interference pulses and missing pulses.

  9. Support Group for Parents Coping with Children with Type 1 Diabetes / Skupina Za Starše Kot Podpora Družinam Pri Soočanju Z Otrokovo Sladkorno Boleznijo Tipa 1

    Directory of Open Access Journals (Sweden)

    Pate Tanja

    2015-03-01

    Full Text Available Izhodišče. Sladkorna bolezen tipa 1 je ena izmed pogostejših kroničnih bolezni v otroštvu. Optimalno vodenje in presnovna urejenost otrokove sladkorne bolezni sta povezana z aktivno vključenostjo staršev/ skrbnikov, podporo otroku pri nadzoru nad boleznijo in funkcionalnostjo družine. Raziskava predstavlja oceno zadovoljstva staršev s programom skupine za starše in njihovo doživljanje vpliva omenjenega programa na življenje otroka in spoprijemanje z njegovo SBT1.

  10. Pri-Mir-34b/C and Tp-53 Polymorphisms are Associated With The Susceptibility of Papillary Thyroid Carcinoma: A Case-Control Study.

    Science.gov (United States)

    Chen, Peng; Sun, Ruifen; Pu, Yan; Bai, Peng; Yuan, Fang; Liang, Yundan; Zhou, Bin; Wang, Yanyun; Sun, Yinghe; Zhu, Jingqiang; Zhang, Lin; Gao, Linbo

    2015-09-01

    Tumor suppressor p53 directly regulated the abundance of the miR-34b/c. The interaction might contribute to certain cancer. We hypothesized that rs4938723 in the promoter region of pri-miR-34b/c and TP-53 Arg72Pro may be related to the risk of papillary thyroid carcinoma (PTC). A total of 784 patients with PTC and 1006 healthy controls were recruited to participate in this study. The variants were discriminated using a polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). Additionally, the relative expression levels of miR-34b/c and TP-53 in 44 paired samples were revealed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). A significantly increased risk of PTC was observed in the miR-34b/c rs4938723 CT, CC, and CT/CC genotypes compared with the TT genotype (CT vs TT: adjusted odds ratio [OR] = 1.51, 95%confidence interval [CI] = 1.23-1.85; CC vs TT: adjusted OR = 1.89, 95%CI = 1.39-2.63; CT/CC vs TT: adjusted OR = 1.59, 95%CI = 1.30-1.92, respectively). Significantly increased PTC susceptibility was also associated with the TP-53 Arg72Pro CC and CG/CC genotypes compared with the GG genotype (CC vs GG: adjusted OR = 2.04, 95%CI = 1.54-2.70; CG/CC vs GG: adjusted OR = 1.35, 95%CI = 1.11-1.67, respectively). Stratification analysis revealed that patients carrying the TP-53 Arg72Pro C allele and CC genotype had a significantly increased risk for developing N1 (C vs. G: OR = 1.27, 95%CI = 1.03-1.56; CC vs. GG: OR = 1.62, 95%CI = 1.07-2.46, respectively). Combined analysis showed that the genotypes of rs4938723 CT/CC + TP-53CG/CC increased the risk of PTC compared with rs4938723TT + TP-53GG (OR = 2.25, 95%CI = 1.67-3.03). Additionally, level of miR-34b was significantly upregulated in PTC patients.These findings indicate that the miR-34b/c rs4938723 and TP-53 Arg72Pro polymorphisms may contribute to the susceptibility of PTC.

  11. A Simple and High-Throughput Analysis of Amatoxins and Phallotoxins in Human Plasma, Serum and Urine Using UPLC-MS/MS Combined with PRiME HLB μElution Platform.

    Science.gov (United States)

    Zhang, Shuo; Zhao, Yunfeng; Li, Haijiao; Zhou, Shuang; Chen, Dawei; Zhang, Yizhe; Yao, Qunmei; Sun, Chengye

    2016-05-04

    Amatoxins and phallotoxins are toxic cyclopeptides found in the genus Amanita and are among the predominant causes of fatal food poisoning in China. In the treatment of Amanita mushroom poisoning, an early and definite diagnosis is necessary for a successful outcome, which has prompted the development of protocols for the fast and confirmatory determination of amatoxins and phallotoxins in human biological fluids. For this purpose, a simple, rapid and sensitive multiresidue UPLC-MS/MS method for the simultaneous determination of α-amanitin, β-amanitin, γ-amanitin, phalloidin (PHD) and phallacidin (PCD) in human plasma, serum and urine was developed and validated. The diluted plasma, serum and urine samples were directly purified with a novel PRiME technique on a 96-well μElution plate platform, which allowed high-throughput sample processing and low reagent consumption. After purification, a UPLC-MS/MS analysis was performed using positive electrospray ionization (ESI+) in multiple reaction monitoring (MRM) mode. This method fulfilled the requirements of a validation test, with good results for the limit of detection (LOD), lower limit of quantification (LLOQ), accuracy, intra- and inter-assay precision, recovery and matrix effects. All of the analytes were confirmed and quantified in authentic plasma, serum and urine samples obtained from cases of poisoning using this method. Using the PRiME μElution technique for quantification reduces labor and time costs and represents a suitable method for routine toxicological and clinical emergency analysis.

  12. A Simple and High-Throughput Analysis of Amatoxins and Phallotoxins in Human Plasma, Serum and Urine Using UPLC-MS/MS Combined with PRiME HLB μElution Platform

    Directory of Open Access Journals (Sweden)

    Shuo Zhang

    2016-05-01

    Full Text Available Amatoxins and phallotoxins are toxic cyclopeptides found in the genus Amanita and are among the predominant causes of fatal food poisoning in China. In the treatment of Amanita mushroom poisoning, an early and definite diagnosis is necessary for a successful outcome, which has prompted the development of protocols for the fast and confirmatory determination of amatoxins and phallotoxins in human biological fluids. For this purpose, a simple, rapid and sensitive multiresidue UPLC-MS/MS method for the simultaneous determination of α-amanitin, β-amanitin, γ-amanitin, phalloidin (PHD and phallacidin (PCD in human plasma, serum and urine was developed and validated. The diluted plasma, serum and urine samples were directly purified with a novel PRiME technique on a 96-well μElution plate platform, which allowed high-throughput sample processing and low reagent consumption. After purification, a UPLC-MS/MS analysis was performed using positive electrospray ionization (ESI+ in multiple reaction monitoring (MRM mode. This method fulfilled the requirements of a validation test, with good results for the limit of detection (LOD, lower limit of quantification (LLOQ, accuracy, intra- and inter-assay precision, recovery and matrix effects. All of the analytes were confirmed and quantified in authentic plasma, serum and urine samples obtained from cases of poisoning using this method. Using the PRiME μElution technique for quantification reduces labor and time costs and represents a suitable method for routine toxicological and clinical emergency analysis.

  13. Ostaliss pri svoihh / Aleksandr Shegedin

    Index Scriptorium Estoniae

    Shegedin, Aleksandr

    2005-01-01

    Ülevaade Riigikogus toimunud pressikonverentsist, kus Riigikogu välisasjade komisjoni esimees Marko Mihkelson ja Venemaa Riigiduuma väliskomitee esimees Konstantin Kossatshov vastasid Eesti-Vene suhteid ning Riigiduuma delegatsiooni visiidi tulemusi puudutavatele küsimustele

  14. Animal Representations on Ceremonial Objects Found in the Tumulus at Črnolica pri Šentjurju at the Foot of Rifnik

    Directory of Open Access Journals (Sweden)

    Iztok Vrenčur

    2013-07-01

    Full Text Available The years 1985 and 1986 saw a rescue excavation by archaeologists from the Institute for the Protection of Cultural Heritage of Slovenia, Celje Regional Office, performed on the severely damaged Iron Age tumulus in the village of Črnolica pri Šentjurju. The village lies in the close vicinity of the Rifnik hill, the site of one of the largest known hilltop settlements in Slovenia dating from the Late Bronze and Iron Ages. The excavation in Črnolica yielded a stone grave chamber of monumental dimensions, together with fragmented and decontextualised grave goods. These included a large set of quality ceramic vessels, a considerable number of bronze vessels, glass cups, fibulae, and a horse harness. Of special importance are three bronze items fabricated in Etruria, which carry the protomas of an aquatic bird, horses, and a ram. The burial dates to the Hallstatt C2 period or the second half of the 7th century BC. Aquatic birds are positioned on all four vertexes of the presentatoio, a special type of vessel presumably used for libation rituals. Comparable items have come to light in the richest graves of the Iron Age aristocracy of Etruria, Latium Vetus and Bologna, and date to the end of the 8th and the beginning of the 7th centuries BC. The aquatic bird sculptures on the presentatoio are stylised, displaying features of the Late Italic Geometric style. This motif marks various Late Bronze and Iron Age items associated with ritual activities in the greater part of Europe, connecting them with prehistoric religious beliefs. While the assumption that they refer to the sun divinisation cult is not proven, they must have signified some prehistoric aspect of the holy or numinous. The two anthropomorphic legs with an attached horse protoma found in the Črnolica tumulus were reconstructed as a part of a tripod of small dimensions, which can be classified as a member of the so-called horse tripod family, fine products of the Italic Geometric style

  15. Anxiety, Self-Esteem and Coping With Stress in Secondary School Students in Relation to Involvement in Organized Sports / Anksioznost, Samospoštovanje In Spoprijemanje S Stresom Pri Srednješolcih V Povezavi Z Vključenostjo V Organizirano Športno Aktivnost

    Directory of Open Access Journals (Sweden)

    Dolenc Petra

    2015-09-01

    Full Text Available Cilj. Namen raziskave je bil preučiti raven samospoštovanja in anksioznosti ter uporabo različnih strategij spoprijemanja s stresom pri srednješolskih mladostnikih v povezavi z vključenostjo v redno in organizirano športno vadbo.

  16. Fazna ravnoteža alkalijskih nitrata (NaNO3, KNO3, RbNO3, CsNO3) u sustavu glicerol + H2O pri različitim temperaturama

    OpenAIRE

    Zhang, H.-Y.; Li, S.-N.; Zhai, Q.-G.; Ou, Y.-M.; Jiang, Y.-C.; Hu, M.-C.

    2014-01-01

    Uređajem vlastite izrade mjerena je topljivost alkalijskih nitrata (NaNO3/KNO3/RbNO3/CsNO3) u smjesi otapala glicerol [HOCH2CH(OH)CH2OH] + H2O u ovisnosti o masenom udjelu glicerola (0,0 – 1,0) pri 35 i 45 °C. Određene su gustoće i indeksi loma zasićenih otopina NaNO3/KNO3/ RbNO3/CsNO3 + smjesa otapala glicerol + H2O. Eksperimentalni rezultati su pokazali da se s povećanjem masenog udjela glicerola u svim sustavima smanjuju topljivost soli i gustoća otopine, dok se indeks loma postupno poveća...

  17. 一种基于PRI变换的雷达信号分选方法研究%Research into A Radar Signal Sorting Method Based on PRI Transform

    Institute of Scientific and Technical Information of China (English)

    徐梁昊; 姜秋喜; 潘继飞

    2014-01-01

    阐述了雷达信号分选的参数和方法,论述了直方图算法、脉冲重复周期(PRI)变换法的原理,研究了PRI变换法及其改进算法的性能,利用Matlab软件进行仿真实验,验证了改进后的PRI变换法能克服PRI抖动带来的信号分选困难,为下一步建立雷达对抗分选模型和采用主分选算法提供了参考依据.

  18. PRI变化雷达信号相位差变化率定位法研究%Research into The Location Methods of Phase Difference Rate to The Radar Signal with The Variational PRI

    Institute of Scientific and Technical Information of China (English)

    张国毅; 宋德亮; 陈枫

    2010-01-01

    目前相位差变化率定位法的研究大都假设目标信号脉冲重复间隔(PRI)恒定,没有考虑到PRI变化引起的相位差变化率误差,而实际雷达信号PRI变化最为常见.研究了PRI变化信号的相位差变化率提取方法,比较了不同方法的相位差变化率估计精度.仿真结果表明采用最小二乘线性拟合法可以在各种PRI变化信号条件下达到较高的相位差变化率估计精度和定位精度.

  19. 基于TOA差值矩阵的雷达脉冲序列PRI识别方法%PRI Recognition Methods of Radar Pulse Sequences Based on TOA Difference Matrix

    Institute of Scientific and Technical Information of China (English)

    张西托; 饶伟; 李长文

    2009-01-01

    针对雷达脉冲序列的主要特点,利用TOA(脉冲到达时间)差值矩阵探索PRI(脉冲重复间隔)的识别问题.设计了一种新方法,首先由TOA序列求出TOA差值矩阵,再求出TOA差值矩阵的逆矩阵,分析TOA差值矩阵逆矩阵的特殊结构,得到PRI的识别结果.通过研究重频固定、脉冲丢失和重频参差三种情况下的PRI识别问题,说明了该方法用于雷达脉冲序列PRI识别的可行性.

  20. Preliminary evidence for association of genetic variants in pri-miR-34b/c and abnormal miR-34c expression with attention deficit and hyperactivity disorder.

    Science.gov (United States)

    Garcia-Martínez, I; Sánchez-Mora, C; Pagerols, M; Richarte, V; Corrales, M; Fadeuilhe, C; Cormand, B; Casas, M; Ramos-Quiroga, J A; Ribasés, M

    2016-08-30

    Attention deficit and hyperactivity disorder (ADHD) is a prevalent neurodevelopmental disorder characterized by impairment to sustain attention and inability to control impulses and activity level. The etiology of ADHD is complex, with an estimated heritability of 70-80%. Under the hypothesis that alterations in the processing or target binding of microRNAs (miRNAs) may result in functional alterations predisposing to ADHD, we explored whether common polymorphisms potentially affecting miRNA-mediated regulation are involved in this psychiatric disorder. We performed a comprehensive association study focused on 134 miRNAs in 754 ADHD subjects and 766 controls and found association between the miR-34b/c locus and ADHD. Subsequently, we provided preliminary evidence for overexpression of the miR-34c-3p mature form in peripheral blood mononuclear cells of ADHD subjects. Next, we tested the effect on gene expression of single-nucleotide polymorphisms within the ADHD-associated region and found that rs4938923 in the promoter of the pri-miR-34b/c tags cis expression quantitative trait loci for both miR-34b and miR-34c and has an impact on the expression levels of 681 transcripts in trans, including genes previously associated with ADHD. This gene set was enriched for miR-34b/c binding sites, functional categories related to the central nervous system, such as axon guidance or neuron differentiation, and serotonin biosynthesis and signaling canonical pathways. Our results provide preliminary evidence for the contribution to ADHD of a functional variant in the pri-miR-34b/c promoter, possibly through dysregulation of the expression of mature forms of miR-34b and miR-34c and some target genes. These data highlight the importance of abnormal miRNA function as a potential epigenetic mechanism contributing to ADHD.

  1. An autoregulatory network between menin and pri-miR-24-1 is required for the processing of its specific modulator miR-24-1 in BON1 cells.

    Science.gov (United States)

    Luzi, Ettore; Marini, Francesca; Ciuffi, Simone; Galli, Gianna; Brandi, Maria Luisa

    2016-05-24

    Multiple endocrine neoplasia type 1 (MEN1) is a rare hereditary cancer complex syndrome manifesting a variety of endocrine and non-endocrine neoplasms and lesions. MEN1 is characterized by tumours of the parathyroids, of the neuroendocrine cells of the gastroenteropancreatic tract, and of the anterior pituitary. The MEN1 gene, a tumour suppressor gene, encodes the menin protein. Loss of heterozygosity (LOH) at 11q13 is typical of MEN1 tumours in agreement with Knudson's two-hit hypothesis. We previously showed that the MEN1 parathyroid tumorigenesis is under the control of an "incoherent feedback loop" between miR-24-1 and the menin protein that generates a "Gene Regulatory Network" (GRN) that mimics the second hit of Knudson's hypothesis and that could buffer the effect of the stochastic factors that contribute to the onset and progression of this disease. Here we show, in the BON1 cell line derived from lymphnode metastasis of a human carcinoid tumour of the pancreas, that menin binds specifically to the primary RNA sequence pri-miR-24-1 by promoting the miR-24-1 biogenesis. Network simulation showed a new feed-forward loop between menin, microRNA-24-1 and Musashi-1 proteins. This result shows a novel mechanism whereby menin, a RNA-binding protein, facilitates the processing of its specific miRNA by regulating the dynamics of the menin-miR-24 Gene Regulatory Network at the level of pri-miRNA processing.

  2. Zakotvená teória ako východisko pri hľadaní učiteľových stratégií k udržaniu svojej roly v triede

    Directory of Open Access Journals (Sweden)

    Csaba Igaz

    2013-10-01

    Full Text Available Článok sa zaoberá možnosťami využitia zakotvenej teórie pri kvalitatívnej analýze vyučovacích hodín s cieľom identifikovať rolu učiteľa a stratégie, ktoré umožňujú udržanie tejto roly. Takáto analýza sa môže využiť pri zavádzaní nových metód a prostriedkov do vyučovacieho procesu, kedy charakteristika rozdielov v správaní sa učiteľa počas bežnej vyučovacej hodiny a hodiny, v ktorej sa uplatňujú nové metódy nám umožní odhaliť ich slabé stránky. V príspevku sa podrobne venujeme postupu analýzy vyučovacích hodín. Na konkrétnych príkladoch vysvetlíme nielen aplikáciu otvoreného, axiálneho a selektívneho kódovania ale aj tzv. prechodnej úrovne kódovania, ktorá predstavuje nový prvok v zakotvenej teórií.

  3. Two common SNPs in pri-miR-125a alter the mature miRNA expression and associate with recurrent pregnancy loss in a Han-Chinese population.

    Science.gov (United States)

    Hu, Yi; Liu, Chun-Mei; Qi, Lu; He, Tian-Zhu; Shi-Guo, Liu; Hao, Chan-Juan; Cui, Yi; Zhang, Ning; Xia, Hong-Fei; Ma, Xu

    2011-01-01

    Although there are plenty of evidence that single-nucleotide polymorphisms (SNPs) that fall within coding sequences of genes are involved in recurrent pregnancy loss (RPL), it is still unknown whether the polymorphisms in microRNAs (miRNAs) are related with RPL. In this study, we established this kind of association by confirming significant differences in genotype distribution of rs41275794 (P= 0.0005) and rs12976445 (P= 0.001) within the pri-miR-125a in 217 Han Chinese patients of RPL compared with 431 controls. Based on this observation, two-locus haplotypes were constructed and the A-T haplotype was found to be associated with an increased risk of RPL (OR=2.84, 95%C.I. 1.98-4.07, P=0.0000000057). Further analysis showed that the levels of pre- and mature- miR-125a were down-regulated in the cells transfected with the A-T haplotype, which was consistent with in vivo detection that the level of mature miR-125a was lower in 30 pregnant women with A-T haplotype than that with G-C haplotype. During in vitro RNA processing assays, we found a similar decrease in the amount of pre-miR-125a and decline in binding capacity of nuclear factors to pri-miR-125a with A-T haplotype. More importantly, the reduction in miR-125a, as a consequence of A-T haplotype, further led to less efficient inhibition of target genes, LIFR and ERBB2, which play important roles in the embryo implantation and decidualization. Thus, our data collectively suggest that two common polymorphisms in pre-miR-125a might contribute to the genetic predisposition to RPL by disrupting the production of miR-125a, which consequently interfered in the expression and function of target genes of miR-125a.

  4. Estimation of Chlorophyll Contents of Canopy with Advanced PRI Method%基于改进的PRI方法对植被冠层叶绿素含量的反演

    Institute of Scientific and Technical Information of China (English)

    宁艳玲; 张学文; 韩启金; 杨宝祝

    2014-01-01

    叶绿素是植被光合作用的重要色素,对植被的健康状况有直接的指示作用。利用遥感技术准确、快速地获取植被叶绿素含量对精准农业具有重要意义。文章在对各种光化学植被指数(Photochemical Reflectance Index,PRI)与叶绿素含量进行敏感性分析的基础上,提取出对叶绿素变化较为敏感的组合形式,并考虑土壤背景的影响,提出了一种改进的PRI模型,同时利用在小汤山试验田获取的地面采样数据和实用模块化成像光谱仪(Operative Modular Imaging Spectrometer,OMIS)数据进行验证。结果表明,基于OMIS高光谱数据,模型能够很好地对植被冠层的叶绿素含量进行反演,反演的叶绿素含量与实测值具有较好的相关性。%Chlorophyll is an important photosynthesis pigment that directly indicates vegetation’s health status. Using remote sensing technology to estimate chlorophyll content accurately and quickly is important for precision agriculture. On the basis of sensitivity analysis between various photochemical reflectance index (PRI) and chlorophyll content, by extracting more sensitive combination form to chlorophyll and considering the impact of soil background, this paper proposes an improved PRI model. The study tries to use the ground sample data acquisition and synchronization Xiaotangshan airborne hyperspectral sensors OMIS (Operative Modular Imaging Spectrometer) data for validation. The results show that based on the OMIS hyperspectral data, the model can be well used to estimate the chlorophyll contents of vegetation canopy, which have good correlation with the measured values.

  5. Doenças priônicas: avaliação dos riscos envolvidos na utilização de produtos de origem bovina Prionic disease: evaluation of the risks involved in using products of bovine origin

    Directory of Open Access Journals (Sweden)

    Omar Lupi

    2003-02-01

    Full Text Available Os príons são proteínas que se mostram capazes de auto-replicação apesar de, para isso, alterar o metabolismo celular. São responsáveis por inúmeras doenças em animais e no ser humano (doenças priônicas, todas elas fatais. Essas moléstias apresentam enorme variabilidade quanto ao período de incubação, de alguns meses a 40 anos. Os príons acumulam-se e destroem os neurônios, provocando quadros conhecidos como encefalopatias espongiosiformes. Discute-se a apresentação clínica, epidemiológica e histórica das doenças priônicas. O foco maior de discussão recai, no entanto, na possibilidade teórica da transmissão iatrogênica dos príons por meio das formulações tópicas que utilizam ceramidas (cerebrosídeos ou placenta de origem bovina, assim como pelo risco representado por alguns procedimentos dermatológicos, como transplantes da pele e implantes de colágeno.A prion is a protein that is capable of self replication, thereby altering a cell's metabolism. It is responsible for a number of human and animal diseases (prionic diseases, all of which are always lethal. These diseases have enormous variability in their incubation periods, ranging from a few months to forty years. Prions accumulate and destroy nerve cells, causing spongiform encephalopathy. We discuss the clinical picture, epidemiology, and historical background of prionic diseases. The major focus of the discussion lies, however, on the theoretical possibility of iatrogenic transmission of prion infection due to topical formulations using ceramides (cerebrosides or placenta of bovine origin, as well as the risk represented by some dermatological procedures such as skin grafts and collagen implants.

  6. Cold distribution networks. Cold distribution by two-phase refrigerant fluid. Case of ice slurries; Reseaux de distribution de froid. Distribution de froid par fluide frigoporteur diphasique. Cas du coulis de glace stabilisee (2002 - 2004). Rapport final (1er juillet 2004). Programme energie. PRI 9.1

    Energy Technology Data Exchange (ETDEWEB)

    Lallemand, A. [Institut National des Sciences Appliquees (INSA), Centre de Thermique de Lyon (CETHIL), UMR 5008, 69 - Villeurbanne (France); Mercier, P. [CEA Grenoble, Groupement pour la Recherche sur les Echangeurs Thermiques (GRETH), 38 (France); Royon, L. [Paris-1 Univ., Lab. de Biorheologie et d' Hydrodynamique Physico-Chimique, 75 (France); Dumas, J.P. [L' Ecole Nationale Superieure en Genie des Technologies Industrielles, Lab. de Thermique Energetique et Procedes, 64 - Pau (France); Guilpart, J. [URGPAN/CEMAGRE, 33 - Bordeaux (France)

    2004-07-01

    This PRI aims to participate to the development of alternate solutions for refrigerant fluids, for the cold transport and more specially a two phase refrigerant fluid: the stabilized ice slurry. The research program presented three main axis: design, realization and characterization of stabilized ice slurry, experimental studies of transport and transfer properties, study of online measurement process of the solid content. A simulation has been realized to evaluate the energy efficiency of this refrigerant use. (A.L.B.)

  7. Association of Average Telomere Length with Body-Mass Index and Vitamin D Status in Juvenile Population with Type 1 Diabetes / Povezava Povprečnih Dolžin Telomerov Z Indeksom Telesne Teže in Vitaminom D Pri Mladostnikih S Sladkorno Boleznijo Tipa 1

    Directory of Open Access Journals (Sweden)

    Tesovnik Tine

    2015-03-01

    Full Text Available Izhodišče. Sladkorna bolezen tipa 1 (SBT1 je kronična avtoimunska bolezen, pri kateri hiperglikemija ter zvišana raven oksidativnega stresa in končnih produktov glikacije skupaj z genetskimi in okoljskimi dejavniki privedeta do nastanka diabetičnih zapletov. Krajše dolžine telomerov so povezane s hiperglikemičnimi epizodami in nižjimi serumskimi vrednostmi vitamina D.

  8. vsiRNAs derived from the miRNA-generating sites of pri-tae-miR159a based on the BSMV system play positive roles in the wheat response to Puccinia striiformis f. sp. tritici through the regulation of taMyb3 expression.

    Science.gov (United States)

    Feng, Hao; Zhang, Qiong; Li, Huayi; Wang, Xiaojie; Wang, Xiaodong; Duan, Xiaoyuan; Wang, Bing; Kang, Zhensheng

    2013-07-01

    Plants live in a complex environment, exposed to stresses, such as unsuitable climates, pests and pathogenic microorganisms. Pathogens are one of the most serious factors that threaten plant growth. Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases worldwide. Virus-induced gene silencing (VIGS) is a popular tool for the functional analysis of wheat genes, generating abundant small RNAs (sRNAs). sRNAs are key components in gene regulatory networks, silencing corresponding genes at the post-transcriptional level. In this study, we transduced pri-tae-miR159a into plant tissues using the barley stripe mosaic virus (BSMV) system, and demonstrated that vsiRNAs were generated from the same miRNAs generating sites of pri-tae-miR159a, with the function of Dicer RNase III-like classes of endonucleases (DCL4). In addition, the accumulation of vsiRNAs in wheat leaves challenged with Pst Chinese yellow rust 23 (CYR23), resulted in a resistant phenotype, and in the compatible interaction, the sporation of Pst was limited. Whereas, infection with a control construct had no effect on the resistance or susceptibility. The results of the histological observation also supported these phenotype changes. Interestingly, vsiRNAs were also involved in the interactions between wheat and Pst through the tae-miR159-mediated regulation of taMyb3 expression. Moreover, these results also supported the speculation that vsiRNAs were generated from the same sites of pri-tae-miR159a. These studies indicated that vsiRNAs from miRNAs generating sites of pri-tae-miR159a based on the BSMV system play positive roles in the wheat response to Pst through the regulation of taMyb3 expression.

  9. Effect of phase separation induced supercooling on magnetotransport properties of epitaxial La5/8−yPryCa3/8MnO3 (y≈0.4 thin film

    Directory of Open Access Journals (Sweden)

    Sandeep Singh

    2015-02-01

    Full Text Available Thin films of La5/8−yPryCa3/8MnO3 (y≈0.4 have been grown on single crystal SrTiO3 (001 by RF sputtering. The structural and surface characterizations confirm the epitaxial nature of these film. However, the difference between the ω-scan of the (002 and (110 peaks and the presence of pits/holes in the step-terrace type surface morphology suggests high density of defect in these films. Pronounced hysteresis between the field cooled cooling (FCC and field cooled warming (FCW magnetization measurements suggest towards the non-ergodic magnetic state. The origin of this nonergodicity could be traced to the magnetic liquid like state arising from the delicacy of the coexisting magnetic phases, viz., ferromagnetic and antiferromagnetic-charge ordered (FM/AFM-CO. The large difference between the insulator metal transitions during cooling and warming cycles (TIMC ∼ 64 K and TIMW ∼ 123 K could be regarded as a manifestation of the nonergodicity leading to supercooling of the magnetic liquid while cooling. The nonergodicity and supercooling are weakened by the AFM-FM phase transition induced by an external magnetic field. TIM and small polaron activation energy corresponding the magnetic liquid state (cooling cycle vary nonlinearly with the applied magnetic field but become linear in the crystalline solid state (warming cycle. The analysis of the low temperature resistivity data shows that electron-phonon interaction is drastically reduced by the applied magnetic field. The resistivity minimum in the lower temperature region of the self-field warming curve has been explained in terms of the Kondo like scattering in the magnetically inhomogeneous regime.

  10. REVIEW OF RETRIEVAL LIGHT USE EFFICIENCY USING PHOTOCHEMICAL REFLECTANCE INDEX (PRI)%光化学植被指数估算植物光能利用率的研究进展

    Institute of Scientific and Technical Information of China (English)

    吴朝阳; 牛铮

    2008-01-01

    应用遥感技术可以建立光化学植被指数(Photochemical reflectance index, PRI)和光能利用效率(Light use efficiency, LUE)的关系,LUE可用来估算净初级生产力(Net primary productivity, NPP).因而,用PRI估算植物的LUE,为估算NPP提供了新的方法,弥补了以往以经验模式通过温度和水分对最大LUE的胁迫来获取实际比LUE的不足,进而可提高NPP的估算精度.研究表明:PRI和LUE在叶片、冠层和景观尺度上都有着很好的相关性,但是随着尺度的变化,很多因素会对这一关系产生干扰,如水分、氮元素含量、叶面积指数和太阳高度角等,从而削弱了PRI和LUE的关系.该文对建立PRI和LUE的关系过程中的影响因素进行了分析,并指出今后这一研究领域中可能改进的方面,主要包括526nm和545nm处的反射率对531nm处的反射率的作用机制、PRI随LUE的饱和现象、PRI和LUE关系的时间效应以及利用PRI估算LUE的尺度效应.

  11. Vernost reprodukcije boja pri digitalizaciji slikovnih podataka

    OpenAIRE

    Živković, Predrag; Vujaklija, Vanja

    2006-01-01

    This paper emphasis the importance of standardization of digitalization process, in the particular case digitalization the colorized object, when information of object color is of great impotence. Some prepositions for standardization of digitalization process of colorized objects are also explained.

  12. PRI EDZINO-NOMOJ EN HUNGARIO

    Institute of Scientific and Technical Information of China (English)

    A.T.Trad.

    1997-01-01

    La edzini o signifas nomo an an eblon porla virinoj en Hungario.Sed tio estas ne deviga.ian elekton povas motivi pluraj vidpunktoj.Enla konservado de ia nomo povas roli ekzemptetio,ke i atus daǔrigi sian artan karieron kun

  13. Pri delezhke Tsheburashki mehh letit po storonam

    Index Scriptorium Estoniae

    2007-01-01

    Vene ärimees ostab ameerika kompaniilt Films by Jove kollektsiooni nõukogude multifilmide (547 filmi) levitamisõigused, et anda need üle loodavale laste telekanalile Venemaal. Lähemalt laste multifilmide lemmikkangelasest Tsheburashkast, mille loojaks kirjanik Eduard Uspenski

  14. Pri la 81-a UK en Prago

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    Antaǔtagmeze de la 21-a de aǔg.okazis enla mil-tura urbo Prago 1a 81-a Universala kon-greso de Esperanto kon prezidanto de la (?)e(?)aRespubliko s-ro Vaclav Havel kiel alta protek-tanto.UEA-prezidanto Lee Chong-Yeong

  15. Prevajanje termina ratio pri Varonu in Kvintilijanu

    Directory of Open Access Journals (Sweden)

    Kozma Ahačič

    2000-12-01

    Full Text Available Termin (Lewandowski 1994: 1152-1153 je izraz, katerega pomen je enoznačno definiran. Vedno je v natančno opisanem razmerju z drugimi termini določene terminologije. Enopomenskost termina je, v nasprotju z običajnimi besedami, neodvisna od konteksta; lahko ga rabimo tudi izolirano. Ko postane beseda termin, zoži in specializira svoj (dotedanji pomen - tako rekoč izstopi iz svojih dotedanjih semantičnih povezav. Dobimo - če uporabimo izrazoslmje Umberta Eca - pomensko kastrirano besedo.

  16. Hiding the Higgs Boson from Prying Eyes

    CERN Document Server

    Ma, Ernest

    2011-01-01

    There are two ways that the Higgs boson H of the Standard Model (SM) may be more difficult to observe than expected at the Large Hadron Collider (LHC) or the Tevatron. One is well-known, i.e. H decays invisibly, into dark-matter scalar particles for example. The other is that H mixes with a heavy singlet scalar S which couples to new colored fermions and scalars. Of the two mass eigenstates, the lighter one could (accidentally) have a suppressed effective coupling to two gluons, and the heavy one could be kinematically beyond the reach of the LHC.

  17. Tak prinjato pri demokratii / Julia Rodina

    Index Scriptorium Estoniae

    Rodina, Julia

    2006-01-01

    Avatud Eesti Fondi poolt läbi viidud uuring demokraatia toimimisest Eestis: mitmed parlamendiliikmed peavad ajakirjandust segavaks faktoriks, mis seab ohtu demokraatia, politoloogide ja sotsioloogide hinnanud poliitikute ja meedia suhetest

  18. Report of the PRI biofuel-ethanol; Rapport du PRI biocarburant-ethanol

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    This evaluation report presents three research programs in the framework of the physiological behavior of the yeast ''Saccharomyces cerevisiae'', with high ethanol content. These studies should allowed to select an efficient yeast for the ethanol production. The first study concerns the development of an enzymatic process for the hydrolysis and the fermentation. The second study deals with the molecular and dynamical bases for the yeast metabolic engineering for the ethanol fuel production. The third research concerns the optimization of performance of microbial production processes of ethanol. (A.L.B.)

  19. Treatment of linguiform calcaneus fracture by close nail-pry reduction and internal fixation with hollow screws%闭合撬拨复位经皮空心螺钉内固定治疗跟骨舌形骨折

    Institute of Scientific and Technical Information of China (English)

    涂淑强; 黄科棣; 帅永明; 徐南云; 袁秋文; 郭剑

    2012-01-01

    Objective: To study the curative effects of close nail-pry reduction and internal fixation with hollow screws for treatment of linguiform calcaneus fracture. Methods: From May 2006 to October 2009,32 patients (35 feel) with linguiform calcaneus fracture were treated by close nail-pry reduction and internal fixation with hollow screws, including 23 males and 9 females ranging in age from 25 to 46 years, with a mean of 37.6 years. According to Paley classification, 3 cases were Paley II a, and 29 cases were Paley II b. All cases were close fractures. The time from injury to operation was 3 to 10 days after most swelling subsided. BBhler angle and Gissane angle were measured by X-ray before and after operation. The therapeutic effect was assessed according to ZHANG Tie-liang's foot score. Results:All the patients were followed-up for 6 to 18 months,with a mean of 12 months. All fractures gained bone healing. The time of fracture healing averaged 12 months. The fractures healed completely and no infection occurred. According to ZHANG Tie-liang's foot scale,the postoperative function was excellent in 18 feet,good in 10 feet, moderate in 5 feet and poor in 2 feet. The Bohler angle and Gissane angle were significant improved after treatment (P<0.01 ). Conclusion: The surgical method of close nail-pry reduction and internal fixation with hollow screws for treatment of linguiform calcaneus fracture can regain the foot function, with minimal injury, fewer complications, earlier recovery and lower costs.%目的:探讨闭合撬拨复位经皮空心螺钉内固定治疗跟骨舌形骨折的手术疗效.方法:自2006年5月至2009年10月共收治32例(35足)跟骨舌形骨折,男23例,女9例;年龄25~46岁,平均37.6岁.根据Paley分类法:PaleyⅡa型3例,PaleyⅡb型29例,均为闭合性骨折.手术时间为伤后3~10d,均等局部肿胀消退至中、轻度后手术.通过X线片测量手术前后B(o)hler角及Gissane角,并按张铁良足部综

  20. Metodologija postavljanja diferencijalnih jednačina pri istraživanju dinamičkih parametara konstrukcije lansirne rampe na vozilu točkašu / Methodology make of differential equations at investigation of dynamic parameters of constructions of launcher on vehicle

    Directory of Open Access Journals (Sweden)

    Vlado P. Đurković

    2007-07-01

    Full Text Available U radu se određuju optimalni parametri konstrukcije lansirne rampe: položaj tačke vešanja hidrocilindra na rampi, dužina i materijal rampe, koeficijent viskoznog trenja ulja u hidrocilindru, koeficijent krutosti lansirne rampe i hidrocilindra, poprečni presek rampe, itd. Radi toga postavlja se mehanički model sa tri stepena slobode kretanja i odgovarajući model u vidu sistema od tri nelinearne diferencijalne jednačine drugog reda. Numeričkom analizom dobijenog matematičkog modela (primenom programskog jezika Compaq Visual Fortran, Version 6.5 dolazi se do optimalnosti pojedinih parametara. Dobijeni rezultati, predstavljeni u grafičkoj formi, mogu da budu veoma korisni projektantima raketnih lansera, kako stabilnih, tako i mobilnih, pri razvoju novih konstrukcija i modifikaciji postojećih. / This paper determines optimal construction parametrics of a missile launcher place of hydro-cylinder on launcher, length and material of ramp of launcher coefficient of the viscosity of friction oil in hydro-cylinder, coefficient of stiffness of launcher and hydro-cylinder, cross-section of launcher etc. In this purpose appointment mechanical model with three degrees of freedom motion and analogous model of system of three nonlinear differential equation second order. Numerical analysis obtained mathematical model (programming with language Compaq Visual Fortran, Version 6.5 coming to optimal parameters. Obtained results that are presented in graphical shapes can be very useful for designing stable and mobile missile launchers, both for development of new constructions and modification of existing structures.

  1. Funkcija jetre i bubrega pri trovanju molibdenom i bakrom

    OpenAIRE

    Rana, S.V.S.; Kumar, A.

    1983-01-01

    Autori iznose rezultate testiranja funkcije jetre i bubrega (ukupni bilirubin, hipurna kiselina, glukoza-6-fosfataza, kolinesteraza, urea u serumu, albumin u mokraći, specifična težina mokraće i dilucijski test) koje su proveli da bi otkrili eventualna oštećenja jetre i bubrega štakora koji su primali hranu s dodatkom molibdena i bakra. Vrijednosti bilirubina u štakora hranjenih hranom s dodatkom bakra upućivali su na hemolizu. Niske razine hipurne kiseline također su ukazivale na oštećenje j...

  2. VPLIV NADZORA ZAPOSLENIH NA NJIHOVO VEDENJE IN MOTIVACIJO PRI DELU

    OpenAIRE

    Marinšek, Teja

    2011-01-01

    Nadzor na delovnem mestu je sodoben problem, v katerem smo udeleženi vsi. Z vedno manjšim zaupanjem med nadrejenimi in podrejenimi se nadzor poostruje in s tem se tudi pritiski na delovnem mestu povečujejo. V diplomskem delu smo raziskovali predvsem vpliv nadzora na vedenje človeka. Zanimalo nas je, kako tak pritisk vpliva na človeka in njegove spremembe v obnašanju. Ali nanj to vpliva pozitivno in motivacijsko ali deluje na človeka negativno. Obravnavali smo tudi zakone, ki opredeljujejo...

  3. DISKRIMINACIJA PRI ZAPOSLOVANJU IN DELU NA PODLAGI SPOLNE USMERJENOSTI

    OpenAIRE

    Markovič, Polona

    2012-01-01

    Ustavno zagotovljena pravica do enakosti, brez kakršnegakoli razlikovanja na podlagi osebnih okoliščin, predstavlja eno izmed temeljnih človekovih pravic. Za zagotavljanje enakega obravnavanja posameznikov in različnih skupin oseb na vseh področjih družbenega življenja je neizogibno potrebna natančna zakonska opredelitev varstva pred diskriminacijo. Prepoved diskriminacije je pravno regulirana v določilih mnogih dokumentov, sprejetih na mednarodni in nacionalni ravni. Diskriminacijo lahko naj...

  4. Kelke da aferoj pri Xu Shouzhen kaj Yang Fuchen

    Institute of Scientific and Technical Information of China (English)

    Fu; Xianpei

    2000-01-01

    Antau 50 jaroj, la 7-an de decembro 1949, martiroj Xu Shouzhen(Eltunko) kaj Yang Fuchen(Yang Qiuzhen) oferis sian vivon al la movado por Esperanto kaj demokratio en Chengdu, la cefurbo de la provinco Sichuan. Xu Shouzhen estis kompetenta cina esperantisto. Komence de la 40-aj jaroj li venis de Chongqing al Chengdu kaj aktivis por nia lingvo. Tiam Li Shijun(Laulum) enhospitaligis en

  5. POMEN EMPATIJE PRI OBRAVNAVI PACIENTA V ENOTI INTENZIVNE TERAPIJE

    OpenAIRE

    Knuplež, Urška

    2015-01-01

    Teoretična izhodišča: V enotah intenzivne terapije so pacienti v kritičnem stanju, zato je potrebno da znamo z njimi vzpostaviti primerno komunikacijo. Komunikacija medicinske sestre mora biti strokovna vendar tudi razumevajoča. To nam omogoča komunikacijska vrlina empatija. Empatija je sposobnost in proces skozi katerega se poskušamo postaviti v življenje in občutke druge osebe, ter gledati situacijo in njegovega zornega kota. Namen raziskave je bil ugotoviti koliko diplomiranih sester/ dipl...

  6. E-Voting and Forensics: Prying Open the Black Box

    OpenAIRE

    Bishop, M.; Peisert, Sean; Hoke, S. Candice; Graff, Mark; Jefferson, David

    2009-01-01

    Over the past six years, the nation has moved rapidly from punch cards and levers to electronic voting systems. These new systems have occasionally presented election officials with puzzling technical irregularities. The national experience has included unexpected and unexplained incidents in each phase of the election process: preparations, balloting, tabulation, and reporting results. Quick technical or managerial assessment can often identify the cause of the problem, leading to a simple ...

  7. Zdravstvena nega onkološkega bolnika pri sistemski terapiji

    OpenAIRE

    Korez, Sanja

    2014-01-01

    Onkološka zdravstvena nega je kompleksno in razvejano področje zdravstvene nege, na katerega vplivajo tudi druge specialne vede. Rak je splošno ime za obsežno skupino različnih bolezni, katerih glavna značilnost je nenadzorovana razrast spremenjenih, rakastih celic. Benigen tumor je grajen iz celic, podobnih normalnim, in lahko raste omejeno. Nasprotno pa so maligni tumorji rakasti. Grajeni so iz nenormalnih celic, ki se neurejeno in nenadzorovano širijo. Razraščajo se v sosednja tkiva in...

  8. Pri tofuo/tohuo/toǔfuo kai soikazeo

    Institute of Scientific and Technical Information of China (English)

    AHN Song-san

    1997-01-01

    Manajoj el la t.n.sojfabo estas popularakaj gravaj en la orienta Azio,por inoj,japanojkaj koreoj.Science kaj praktike,la plantoj fabo kajsojfabo ne estas samaj legumenacaj specioj.Lavorto fabo(Vicia faba L.)estas uzata anka

  9. Bronzovaja medal pri "derevjannõhh" zarplatahh / Kirill Adejev

    Index Scriptorium Estoniae

    Adejev, Kirill

    2006-01-01

    Rahvusvaheline firma Nau Group uuris juhtide sissetulekuid erinevates maades. Uuringu andmetel teenivad juhid Venemaal aastas keskmiselt 77,4 tuhat eurot ja on sellega firma poolt koostatud pingereas, kuhu kuulub 29 riiki, kolmandal kohal

  10. Desjatiletije galerei pri russkom teatre 11 maja / Liana Turpakova

    Index Scriptorium Estoniae

    Turpakova, Liana

    2006-01-01

    Vene Draamateatri galerii korraldatud näituse "Natüürmort" avamine juveeligaleriis Bindes sõnas ja pildis. Fotod Aleksei Belovilt. Näitusega tähistati galerii kümnendat aastapäeva ja galerist Olga Ljubaskina sünnipäeva

  11. Organizacija inžinjerijskih radova pri rušenju deonice puta / Organization of engineering works during demolition of a road section / Организация инженерных работ по демонтажу участков дорог

    Directory of Open Access Journals (Sweden)

    Nenad V. Kovačević

    2016-01-01

    Full Text Available Prilikom upotrebe minsko-eksplozivnih sredstava za rušenje elemenata, materijala i objekata jako je bitna organizacija izvođenja radova prilikom pripreme i u toku izvođenja samog rušenja. Za izvođenje ovih radova u Vojsci Srbije postoje specijalizovane i namenske jedinice. Radi se o jedinicama jedne od specijalnosti u okviru roda inžinjerije, pionirske specijalnosti, koje egzistiraju u okviru inžinjerijskih bataljona, odnosno brigada KoV. Organizacija inžinjerijskih radova zapravo predstavlja iznalaženje najcelishodnijeg rešenja u pogledu upotrebe pokretnih stvari, ali i naprezanja ljudstva, pri rušenju konkretnog objekta – deonice puta. Pri tome veliki problem predstavlja literatura, a posebno norme za izvođenje radova, jer izuzev nekoliko skripti za internu upotrebu, sve se svodi na literaturu iz sedamdesetih ili početak osamdesetih godina prošlog veka. Posebno je razrađena primena mera zaštite prilikom upotrebe eksploziva. / When using explosive ordnance for the demolition of elements, materials and facilities, organization is very important in the preparation of works as well as during demolition itself. In the Army of Serbia, for this kind of works, there are specialized expert units within Land Forces engineering battalions or brigades. The organization of the engineering work given in the article is a purposeful solution, regarding the use of movable property and stress imposed on people as well, when performing demolition of a concrete structure - road section. Lack of literature is a big drawback, especially lack of norms for demolition works. Except for some unofficial lectures for internal use, most of the literature dates back to the seventies or early eighties. A special emphasis in this paper is given to the application of protective measures in the use of explosives. / В процессе применения минно-взрывных средств для сноса элементов, материалов

  12. Do PRI ao sistema plural na transição mexicana From PRI's hegemony to a plural system in the mexican transition

    Directory of Open Access Journals (Sweden)

    Nelson Rojas de Carvalho

    1997-08-01

    Full Text Available Os resultados das eleições mexicanas de 6 de julho deste ano devem ser compreendidos à luz das políticas de ajuste e de reforma do Estado implementadas por De La Madrid e Salinas de Gortari. A reforma do Estado minou o partido de Estado: a perestroika mexicana acabou por produzir sua glasnostThe outcome of this year's Mexican elections must be understood from the perspective of the structural reform policies put into practice by presidents De La Madri and Salinas de Gortari. The reform of the State undermined the state party: the Mexican perestroika produced its own glasnost.

  13. Izkušnja razkritja pri istospolno usmerjenih odraslih in njihovih starših

    OpenAIRE

    Hikel, Tadeja

    2015-01-01

    V diplomskem delu sem se osredotočila na istospolno usmerjene osebe, ki so svojo istospolno identiteto že razkrile svojim staršem in na starše, ki imajo za sabo že izkušnjo soočenja z otrokovo istospolno usmerjenostjo. Zanimalo me je predvsem, kako istospolno usmerjene osebe doživljajo lastno razkritje in kako starši doživljajo razkritje svojega otroka. V teoretičnem delu se osredotočam predvsem na nastajanje istospolne identitete, na njeno razkritje ter vpliv le tega na odnos starši – ot...

  14. Novi pristopi pri proučevanju prsti v pokrajini = New approaches in Slovenian soil geography

    Directory of Open Access Journals (Sweden)

    Blaž Repe

    2006-01-01

    Full Text Available In Slovenia, soil research of a given landscape is often neglected in physical geography studies. Despite the fact of an equivalent position of soil geography within the science system of Slovene geography. Reasons can be found in time consuming fi eld research, expensive laboratory analysis and soil data and also the lack of its own methodology. The drawbacks could be partially replaced by different approaches of research and especially to establish links with other physical elements of the environment. The use of easily accessible digital and cartographic data, basic field techniques, in combination with simple GIS tools and quantitative methods overcomes many of the financial or time constraints.

  15. EFFEKTIVNOST' VNUTRISUSTAVNOGO VVEDENIYa GENTAMITsINA PRI PODAGRIChESKOM ARTRITE

    Directory of Open Access Journals (Sweden)

    A. V. Orlov-Morozov

    2000-01-01

    Full Text Available Aim: То determine the efficacy of intraarticular injections of Gentamycin in gout arthtiris a randomized double blind, placebo-controlled trial was carried out. Material and methods: 27 patients answering the criteria of the definite gout diagnosis were randomly recruited from rheumatological clinic. The patients received an intraarticular injection of either 4- mg of Gentamycin in 4 ml of sterile 0.25% procaine or 5 ml of the vehicle alone. Results: It was found that weekly intraarticular injections of Gentamycin performed better than the inert vehicle alone over a 2-week period and were conjirmed during 4 week period of crossing-over. Conclusion: The possible mechanisms of Gentamycin activity are discussed. It is concluded, that intraarticular administration of Gentamicin offers signijicant benejit over placebo. The serious side effects were not obsen’ed. Therefore, the technique could be applied in the routine treatment of gout.

  16. Primena metoda ekspertskog ocenjivanja pri oceni profesionalnog rizika / Applying of expert method for professional risk rate

    Directory of Open Access Journals (Sweden)

    Zoran Novaković

    2007-07-01

    Full Text Available Osnovni cilj utvrđivanja ocene profesionalnog rizika je preventivno delovanje u funkciji bezbednosti i zaštite zdravlja zaposlenih, tj. preduzimanje opštih i posebnih mera zaštite na radu radi eliminisanja opasnosti ili štetnosti ili smanjivanja vremena ekspozicije zaposlenog određenim faktorima rizika u sredinama gde je ustanovljena njihova prisutnost. U mnogim slučajevima raspoloživi podaci o prethodnim štetnim događajima su nepotpuni ili nisu dovoljno statistički relevantni. Zbog toga je skup ovih podataka najčešće statistički nehomogen, pa su i rezultati statističke analize, a time i procenjeni rizici, nedovoljno tačni i nepouzdani. Radi toga se u radu predlaže i primenjuje ekspertski metod koji poništava taj nedostatak, a istovremeno omogućava otklanjanje uticaja koji se odnose na neodređenost i subjektivnost pojedinačnih ocena. / The main goal of professional risk rate determination is preventive action in order to secure and protect health of employees. It considers assumption of common and special working protection measures in order to eliminate any danger or damage or to reduce workers exposition time to some risk factors in their working environments. In many cases data on former noxious events are incomplete or statistically insufficiently relevant. So the set of these data is often statistically nonhomogeneous, so are the results of statistical analysis. Thereby rated risks are insufficiently accurate and unreliable. Because of all these things, in this paper is recommend and apply an expert method which annuls this shortage and simultaneously it removes influences that are the consequence of some rate in determination and subjectivity.

  17. 用楔子撬开问题%Using a Wedge to Pry Open a Problem

    Institute of Scientific and Technical Information of China (English)

    马提索夫

    2001-01-01

    When I elicited the Pumi word ts6 'wedge' in Kunming (March 1996), I was struck by its resemblance to Lahu jfl 'wedge'. Since the Qiangic languages are not particularly close to Loloish on the TB family tree,this apparent cognate for an item of non-core vocabulary was of interest. The first task in establishing a relationship between the Pumi and the Lahu forms was to reconstruct the PLB ancestor of Lahu jfi. Then possible cognates to the Pumi form in other Qiangic languages had to be examined.Given our present rudimentary knowledge of comparative Qiangic,could parallel examples establish a Proto-Qiangic reconstruction resembling our newly reconstructed PLB form?

  18. FLAVOUR - Friendly Location-aware Conference Aid with PriVacy Observant ArchitectURe

    NARCIS (Netherlands)

    Muthukrishnan, Kavitha; Meratnia, Nirvana; Lijding, Maria

    2005-01-01

    Context-aware applications are emerging on the daily basis and location information proves to be one of the key components to develop context-aware applications upon. This stems from the fact that location information enables and facilitates reasoning about what users are doing (user's behavioural p

  19. Razvoj odklanjala za rastrsko vodenje žarka pri laserskem označevanju:

    OpenAIRE

    Babnik, Aleš; Bizjak, Aleš; Diaci, Janez

    2007-01-01

    This article presents the development of a new opto-mechatronic system that makes possible the laser marking of raster patterns consisting of several dotted lines. The processing laser beam in our new system is deflected in one direction by a hexagonal mirror prism rotating at a constant angular velocity.Each laser pulse produces a dot-shaped mark (a "laser dot") on the surface of the workpiece. By scanning the beam along a straight line we achieve a linear, uniformly distributed pattern of l...

  20. Razlike pri prevajanju nemško-slovenskih navodil za uporabo zdravil podjetja Bayer

    OpenAIRE

    2014-01-01

    Diplomsko delo predstavlja analizo štirih navodil za uporabo zdravil družbe Bayer, ki se v lekarnah dobijo brez recepta. Besedila so pisana v strokovnem jeziku, kar pomeni, da jih je zaradi strokovne farmacevtske terminologije težje prevajati in zato potrebujejo dodatno pozornost. Kot že samo ime pove, je strokovni jezik v veliki meri namenjen stroki, obstajajo pa besedila, ki so kljub strokovnemu jeziku posplošena in namenjena laikom. Tako so besedila v tem diplomskem delu namenjena ljudem, ...

  1. Optimizatsiya i intensifikatsiya insulinoterapii pri sakharnom diabete 2 tipa (klinicheskie rekomendatsii

    Directory of Open Access Journals (Sweden)

    I I Dedov

    2010-12-01

    Full Text Available По данным Государственного регистра больных сахарным диабетом, на 1 января 2009 года в России сахарный диабет (СД выявлен более чем у 3 миллионов человек, из них 2,7 миллиона приходится на 2 тип. Последние 15-20 лет интерес к выбору оптимальной терапии на всех этапах развития СД 2 типа возрастает. Это связано с одной стороны с появлением новых научных данных, а с другой стороны со всё увеличивающимися возможностями фармакологического воздействия у этой категории больных. В настоящее время специалисты выделяют 9 групп таблетированных и инъекционных препаратов. Эти данные представлены в одном из самых важных опубликованных за последнее время международных документов – совместном консенсусе Американской Диабетической Ассоциации (ADA и Европейской Ассоциации по исследованию диабета (EASD, а также в недавно опубликованном Консенсусе Американской Ассоциации Клинических Эндокринологов (AACE/ Американской Коллегии Эндокринологов (ACE. В этом обзорном документе специально оговаривается сравнение различных препаратов в виде следующего заявления: «В настоящее время недостаточно данных, чтобы поддержать рекомендацию по какому-то конкретному классу сахароснижающих препаратов или их комбинации в отношении влияния на предупреждение осложнений. Поэтому, справедливо судить и сравнивать сахароснижающие агенты, так же, как и их сочетание, в первую очередь по их способности снижать и поддерживать уровень HbA1c, а также по их безопасности, специфическим побочным эффектам, переносимости, удобстве в применении». Далее в документе подчеркивается особая роль инсулина в лечении СД, обосновывая это следующим: • инсулин является самым мощным сахароснижающим препаратом; • своевременное начало инсулинотерапии обеспечивает оптимальный контроль гликемии и, соответственно, улучшает прогноз СД 2 типа, замедляя развитие в организме необратимых изменений; • доказано влияние интенсивной инсулинотерапии на предупреждение развития сосудистых осложнений при диабете; • по мере прогрессирования диабета заболеванием труднее управлять; в этом плане инсулин даёт дополнительные преимущества, поскольку его действие не зависит от остаточной секреторной активности бета-клеток. В настоящих рекомендациях будут затронуты все этапы инсулинотерапии СД 2 типа: начало, оптимизация и интенсификация.

  2. Ocenjevanje displazije kolka pri odraslih: Assessment of hip dysplasia in adults:

    OpenAIRE

    Mavčič, Blaž

    1999-01-01

    Degeneration of the articular cartilage and osteoarthritis of the hip joint are most often idiopathic (primary osteoarthritis). In patients with known pathogenesis (secondary osteoarthritis), the disease is most commonly due to mechanical bone deformation, termed hip dysplasia. The treatment is planned onthe basis of clinical, anatomic and biomechanical characteristics of the hipjoint. The biomechanical pattern is most frequently determined by the Wiberg centre-edge angle of lateral covering ...

  3. Ovlasti i obveze upravnog suca pri pretresu tužbe

    OpenAIRE

    Massot, Jean

    2010-01-01

    Za prikazati što je specifično „dužnosti upravnog suca“ u zemljama kao što je Francuska, treba se osvrnuti na povijesni i zemljopisni prikaz. Izlaže se razloge postojanja upravnog suca u velikom broju demokratskih zemalja i onih koji koračaju prema demokraciji. Ističu se različitosti i specifičnosti upravnog suca u odnosu na ostale suce.

  4. PRAVNI POLOŽAJ ROMSKIH OTROK PRI IZOBRAŽEVANJU V PREKMURJU

    OpenAIRE

    Vučko, Veronika

    2015-01-01

    Republika Slovenija vlaga precej naporov v vključevanje Romov v vrtce in obvezno šolo in v njihovo integracijo v večinsko kulturo na splošno. Predšolski otroci Romov so v slovenske vrtce vključeni na tri načine. Največ jih je integriranih v običajne oddelke. Kljub vsemu je značilen majhen delež romskih otrok, vključenih že v predšolsko vzgojo v vrtcih. Država posebej obravnava in šolam za vzgojo in izobraževanje romskih učencev priznava določene ugodnosti, ki jih bom deloma v nadaljevanj...

  5. Las campañas electorales del PRI en el Distrito Federal

    Directory of Open Access Journals (Sweden)

    Héctor Tejera Gaona

    1999-01-01

    Full Text Available En este artículo se presenta la información derivada del seguimiento de tres campañas del Partido Revolucionario Institucional en el Distrito Federal, previas a las elecciones de julio de 1997. Con el propósito de evaluar el comportamiento ciudadano, se desagregan los diversos elementos que estuvieron en juego en dichas campañas. La información de la relación entre candidatos y ciudadanos es la base para realizar una reflexión sobre las posibilidades de hablar de una nueva cultura política. Se pretende mos¬trar algunos de los contenidos de dicha cultura con base en su expresión en el marco de la contienda electoral, formulando algunas propuestas sobre sus probables efectos en su interrelación con la acción partidaria y gubernamental

  6. "Pri tebi ne bo smelo biti reveža (5 Mz 15,4)"

    OpenAIRE

    Skralovnik, Samo

    2015-01-01

    V obravnavanih mezopotamskih zakonikih, to so Ur-Nammujev, Lipit-Ištarjev, zakonik mesta Ešnunn in Hammurabijev, nobeden od omenjenih zakonodajalcev v pravnem besedilu ne omeni reveža. Zapišejo sicer nekaj besednih zvez, ki se temu približajo, kakor npr. človek v denarni stiski, vendar sama beseda revež ostaja neizrečena. Ob tem se moramo zavedati, da to dejstvo ni posledica šibkega besednega zaklada sumerščine oz. akadščine. Še več, opazimo lahko, da epilog Ur-Nammujevega ter ...

  7. PRI TEBI NE BO SMELO BITI REVEŽA (5 MZ 15,4)

    OpenAIRE

    Skralovnik, Samo

    2009-01-01

    V obravnavanih mezopotamskih zakonikih, to so Ur-Nammujev, Lipit-Ištarjev, zakonik mesta Ešnunn in Hammurabijev, nobeden od omenjenih zakonodajalcev v pravnem besedilu ne omeni reveža. Zapišejo sicer nekaj besednih zvez, ki se temu približajo, kakor npr. človek v denarni stiski, vendar sama beseda revež ostaja neizrečena. Ob tem se moramo zavedati, da to dejstvo ni posledica šibkega besednega zaklada sumerščine oz. akadščine. Še več, opazimo lahko, da epilog Ur-Nammujevega ter prolog Hammurab...

  8. Miniature Electron Sources for Tomorrow’s Vacuum THz Devices (MiPRI)

    Science.gov (United States)

    2006-07-01

    1957). ’F. S. Rusin and G. D. Bogomolov, Proc. IEEE 57, 720 (1969). :8L. A. Weinstein, Radiotekh. Elektron . (Moscow) 2, 883 (1957). 2D. E. Wortman and...following 5L. A. Weinstein, V. A. Isaev, and D. I. Trubetskov, Radiotekh. Elektron . condition: (Moscow) 28, 1233 (1983).6 M. B. Tseitlin and E. A. Myasin...Radiotekh. Elektron . (Moscow) 38, 961 X ej PO 582(1993). S> 155( )2 . (22) 7 V. L. Bratman, I. V. Bandurkin, B. S. Dumesh, A. E. Fedotov, Y K. -sk mfc 1

  9. UPORABA STANDARDA SIST EN ISO 13485:2012 PRI IZDELAVI INDIVIDUALNIH MEDICINSKIH VSADKOV

    OpenAIRE

    Valand, Tomaž

    2013-01-01

    Organizacija, ki izdeluje individualne medicinske vsadke mora imeti vzpostavljen standard nadzora kakovosti v procesu izdelave. Treba je definirati metode in postopke zagotavljanja kakovosti, ki bodo v skladu s standardom ISO 13485. V prvem delu diplomske naloge je predstavljen standard in izdelava individualnega medicinskega vsadka. V drugem delu pa je predstavljena tehnologija izdelave vsadka. Rezultat diplomskega dela je oblikovana in za uporabo pripravljena vsa potrebna dokumentaci...

  10. Depresivnost in anksioznost pri mladostnikih z različnimi duševnimi motnjami:

    OpenAIRE

    Groleger, Urban; Strah-Trotovšek, Darja; Tomori, Martina

    1996-01-01

    In the present study, depression and anxiety were evaluated in a sample of 81 secondary school-aged adolescents, hospitalised at the Adolescent Department, Centre for Mental Health, Ljubljana. The results were compared with those obtained in a control group of 122 secondary school students. The purpose of the study was to identify risk factors for adolescent anxiety and depressive disorders. Data were collected using a clinical interview, the Beck DepressionInventory - BDT and the State and T...

  11. Metoda EMDR kot pomoč policistom pri spoprijemanju s travmatskimi dogodki

    OpenAIRE

    Muraus, Pamela

    2016-01-01

    Travmatski dogodki pogosto vodijo v razvoj posttravmatske stresne motnje – PTSM in so neizogibni del policijskega dela. Da je takšna motnja diagnosticirana in potrjena, mora izpolnjevati osem ključnih kriterijev Diagnostičnega in statističnega priročnika duševnih motenj (DSM − 5). Psihosocialna pomoč policistom je velikokrat spregledana, vendar podprta s podzakonskimi akti, ki jih ponuja njihova institucija. Novodobna metoda, znana pod imenom Desenzitizacija in ponovna predelava z očesnim gib...

  12. Presoja notranjih kontrol pri obračunu davka na dodano vrednost na primeru "Podjetja x"

    OpenAIRE

    Majcen, Borut

    2016-01-01

    Poslovanje podjetja si je ob odsotnosti notranjih kontrol nemogoče predstavljati. Podjetja so podvržena raznim tveganjem, še posebej v današnjih razmerah globalnega poslovanja, ko se podjetja soočajo z vedno novimi izzivi, kar prinaša tudi nova tveganja. Najučinkovitejši ukrep za obvladovanje tveganj predstavlja vzpostavitev sistema notranjih kontrol. Obvladovanje tveganj pa ne poteka samo na ravni podjetja kot celote, ampak je prisotno na vseh področjih delovanja podjetja, torej tudi na p...

  13. UPORABA METOD UMETNE INTELIGENCE PRI ODZIVU NA IZREDNE DOGODKE V SISTEMU RAVNANJA Z ODPADKI

    OpenAIRE

    Kropf, Peter

    2016-01-01

    Ravnanje z odpadki zajema zbiranje, prevažanje, predelavo in odstranjevanje odpadkov, vključno s kontrolo tega ravnanja. Izvajalci si zbiranje in odvoz organizirajo glede na potrebe in razpoložljive vire. Zaradi velikega števila odjemalcev, posod, vozil in voznikov se vnaprej pripravljajo urniki, ki so pripravljeni tako, da se viri znotraj podjetja izkoriščajo optimalno. V fazi izvajanja pogosto pride do nepredvidenih dogodkov, ki onemogočijo normalno izvajanje odvozov po urniku. V dip...

  14. VPLIV JOGE SMEHA NA NIVO GLUKOZE V KRVI PRI LJUDEH S SLADKORNO BOLEZNIJO TIPA 2

    OpenAIRE

    Sternad, Simona

    2013-01-01

    Svetovna zdravstvena organizacija ocenjuje, da ima na svetu kar 246 milijonov odraslih ljudi (5,9 % prebivalstva) sladkorno bolezen, povečuje pa se tudi število otrok s to boleznijo. Dodatno ima še približno 308 milijonov odraslih moteno toleranco za glukozo (7,5 % prebivalstva), ki predstavlja predstopnjo v razvoju sladkorne bolezni, zato so nujni ukrepi za bolj učinkovito obvladovanje sladkorne bolezni. Sladkorno bolezen tipa 2 lahko preprečimo z zdravim načinom življenja. Vsakega posame...

  15. Sodelovanje med marketingom in R&R pri razvoju in uvajanju novih izdelkov:

    OpenAIRE

    Završnik, Bruno; Zupančič, Vinko

    1998-01-01

    To succeed in today's marketplace, most companies must engender cooperation between marketing and R&D functions. Marketing and R&D share responsibilities for setting new product development goals, identifying opportunities for the next generation of product improvement, new engineering design, satisfying and understanding customer needs. Formal and informal integration (cooperation) between marketing and R&D is not guaranty for a successful new product development and introduction into the ma...

  16. Sodelovanje med marketingom in R&R pri razvoju in uvajanju novih izdelkov

    OpenAIRE

    Završnik, Bruno; Zupančič, Vinko

    2015-01-01

    Uspeh podjetja na današnjih zahtevnih trgih je v veliki meri odvisen od sodelovanja med marketingom in R&R. Marketing in R&R si delita odgovornost za postavljanje ciljev razvoja novih izdelkov, iskanje možnosti za nove generacije izdelkov, nove oblike izdelkov, za razumevanje in zadovoljevanje potreb odjemalcev. Formalno in neformalno sodelovanje med marketingom in R&R samo po sebi še ne prispeva k uspešnemu razvoju in uvajanju novih izdelkov na trg. Vodstvo podjetja mora podpirati takšno sod...

  17. Bakteriološka zagađenost alginatnih otisaka i dimenzionalna stabilnost pri dezinfekciji

    OpenAIRE

    Broz, Mira; Romić, Željko; Omrčen, Ante; Fridrih, Sandra; ROPAC, DARKO

    1989-01-01

    Istraživanje mikrobiološke zagađenosti alginatnih otisaka nametnulo je potrebu primjene dezinficijensa prije slanja otisaka u laboratorij. Kao praktično zadovoljavajuće pokazalo se petminutno potapanje otisaka u razrjeđenju od 1:50 klorheksidin-glukonata. Postavljen je problem točnosti uzetog otiska nakon dezinfekcije s obzirom na mogućnost utjecaja dezinfeciejnsa na volumen otiska. Originalnom metodom mjerenja otisne mase dokazano je da promjena volumena i površine alginata nije značajna te ...

  18. Trudovye resursy poslevoennoj Litvy i ih ispol'zovanie pri vosstanovlenii Klajpedy

    Directory of Open Access Journals (Sweden)

    Kretinin G. V.

    2014-05-01

    Full Text Available This article focuses on the issues of post-WWII economic restoration effort in the Soviet Lithuania. German occupation of the republic caused significant damage to its industry and agriculture. Pre-war Lithuania was an agrarian state aspiring to embark on an industrial-agrarian path of development. After the war, this aspiration did not only persist, but was intensified. To reach this objective, however, Lithuania required qualified workforce. Before the war, hardly any attention was paid to the training of workers for industrial-scale production and construction. Then, a considerable decrease in population during the war aggravated the already substantial labour shortage. The attempts of the republic’s leadership to solve the problems of labour shortage through organised labour migration and labour mobilisation yielded no significant results. The appeals to the Centre with the request to send a substantial number of specialists and workers to Lithuania were heard, but a state ravaged by war did not have sufficient human resources. One of the solution was the use of labour of German prisoners of war. A network of prisoner-of-war camps was established in Lithuania. In a matter of two to three years, PWs completed a significant amount of work aimed at the rebuilding of important infrastructural objects. The case of Klaipeda is used to demonstrate the opportunities of the region and Centre in organising workforce in the Lithuanian SSR. The study uses the data obtained by modern historiography and documents kept in the Lithuanian State Central Archive.

  19. Rüütelkond hääbus, sest ei osanud PRi / Kaarel Tarand

    Index Scriptorium Estoniae

    Tarand, Kaarel, 1966-

    2007-01-01

    Rets. rmt.: Jansen, Ea. Eestlane muutuvas ajas seisusühiskonnast kodanikuühiskonda. Tartu : Eesti Ajalooarhiiv, 2007 ; Vahtre, Lauri. Ajaloo pööripäevad I ja II. [Tallinn] : Tammerraamat, 2006 ; 2007

  20. Rahulolev töötaja teeb parimat PRi / Triin Rebane

    Index Scriptorium Estoniae

    Rebane, Triin

    2006-01-01

    Siseturundus on panustamine oma töötajasse, aidates luua firmas positiivse sisekliima, mis annab firmale konkurentsieelise ükskõik millises ärivaldkonnas. Kommenteerib Katrin Alujev. Vt. samas: Maris Lindmäe. Hea meediakoolitus järgib konkreetse kliendi vajadusi; Kaja Pino. Ära unusta pühade ajal töötajaid

  1. Otkrõtije vernisazha "Kak prekrasen etot mir" v galereje pri gostinitse Vana-Wiru

    Index Scriptorium Estoniae

    2008-01-01

    Peterburi kunstniku Marina Printseva tekstiilpiltide ja Odessast pärit kunstniku Juri Gorbatshovi tööde näituse "Kui kaunis on see maailm!" avamine Tallinnas hotell "Vana Wiru" fuajees. Fotod näituse avamiselt

  2. Obrazovanie Vzroslykh pri Probleme Dosuga (Adult Education and the Problem of Leisure)

    Science.gov (United States)

    Darinskii, Anatolii V.

    1969-01-01

    Adult education in the Soviet Union must find flexible new methods, preferably including tourism or other elements of recreation, to raise adults' cultural standards and intellectualize their free time while helping them improve their work qualifications and professional skills. (Author/LY)

  3. Pri vsem bogatstve võborov alternativõ net / Jevgenija Garanzha

    Index Scriptorium Estoniae

    Garanža, Jevgenija, 1979-

    2005-01-01

    Rahvaliit, Keskerakond ja Reformierakond teevad ettevalmistusi algavateks võimukõnelusteks. Turu-uuringufirma Saar Poll poolt läbiviidud uuringust, mille käigus küsiti inimeste arvamusi, kuidas nad käituksid, kui Riigikogu valimised toimuksid homme

  4. Metode socialnega dela s starimi ljudmi in njihova uporaba pri delu z ljudmi z demenco:

    OpenAIRE

    Ambrožič, Maja; Mohar, Špela

    2015-01-01

    Aging population requires new methods of work with older people, forcing us to discover new ways to cope with the challenges posed by aging and old age. The multidisciplinary integration of disciplines is very important because it contributes to the quality of aging and gives older people a voice. Working with the elderly is specific and, specifically in social work, encompasses an important space of research and interweaves theory with practice. In this context, it is necessary to rely on th...

  5. Nonisothermic filtering in working oil fields. Neizotermicheskaya fil'tratsiya pri razrabotke neftyanykh mestorozhdeniy

    Energy Technology Data Exchange (ETDEWEB)

    Alishayev, M.G.; Rozenberg, M.D.; Teslyuk, Ye.V.

    1985-01-01

    A description is made of the effect of water injected in large volumes at low temperature on the development indicators and the oil output coefficient, as well as their dependence on the thermal methods of bed modification. An examination was made of the mechanism for displacing oil from the beds during thermal flooding, temperature mode of the bed beyond the displacement front and in those regions where there is no displacement front. Examples are given of determining the filtering indicators and the technical and technological indicators of working in displacement of oil by water.

  6. Uloga bolesti i štetnika pri odabiru drveća u krajobraznoj arhitekturi

    OpenAIRE

    2005-01-01

    Poznavanje vrsta drveća i njihove osjetljivosti na pojedine bolesti i štetnike, u specifičnim ekološkim uvjetima, od izuzetne je važnosti za njihov odabir u krajobraznoj arhitekturi. Istraživanjima tijekom zadnjih godina u Hrvatskoj zapaženi su značajni problemi sa zdravstvenim stanjem drveća u šumskim ekosustavima, uzrokovani poznatim i do sada manje poznatim uzrocima. U urbanim sredinama utvrđene su slične i još naglašenije promjene na nekim vrstama drveća. Ona su podvrgnuta različitim štet...

  7. ZONA OŠTEĆENJA STIJENSKE MASE PRI ISKOPU TUNELA MINIRANJEM

    OpenAIRE

    Antičević, Hrvoje; Dobrilović, Mario; Perković, Hrvoje

    2012-01-01

    Izrada podzemnih rudničkih prostorija, tunela i odlagališta radioaktivnog otpada, podrazumijevaju primjenu jednakih odnosno sličnih tehnologija. Uslijed iskopa tunela miniranjem dolazi do određenog stupnja oštećenja stijenske mase oko konture presjeka iskopa. Oštećenja stijenske mase koja nastaju u neposrednoj okolini konture presjeka tunela izražene su proširenjem postojećih i stvaranjem novih pukotina, odnosno promjenom fizičko-mehaničkih svojstava stijenske mase. Prilikom izrade dubokih ge...

  8. Sensor4PRI: A Sensor Platform for the Protection of Railway Infrastructures

    Directory of Open Access Journals (Sweden)

    Eduardo Cañete

    2015-02-01

    Full Text Available Wireless Sensor Networks constitute pervasive and distributed computing systems and are potentially one of the most important technologies of this century. They have been specifically identified as a good candidate to become an integral part of the protection of critical infrastructures. In this paper we focus on railway infrastructure protection and we present the details of a sensor platform designed to be integrated into a slab track system in order to carry out both installation and maintenance monitoring activities. In the installation phase, the platform helps operators to install the slab tracks in the right position. In the maintenance phase, the platform collects information about the structural health and behavior of the infrastructure when a train travels along it and relays the readings to a base station. The base station uses trains as data mules to upload the information to the internet. The use of a train as a data mule is especially suitable for collecting information from remote or inaccessible places which do not have a direct connection to the internet and require less network infrastructure. The overall aim of the system is to deploy a permanent economically viable monitoring system to improve the safety of railway infrastructures.

  9. Medsebojno delovanje zdravil pri starostnikih: Drug drug interactions in the elderly:

    OpenAIRE

    Trontelj, Jurij

    2010-01-01

    Elderly patients commonly suffer from comorbidities and often take 4 or more drugs simultaneously. Polypharmacy poses a significant risk for drug-drug interactions. Elderly patients tend to eliminate drugs more slowly and they are more sensitive to drugsć adverse effects at the same time. The most important consequences of drug drug interactions that often require hospitalization are gastrointestinal bleeding, acute bradicardia, hypotension,arrhythmias and falls associated with use of psychot...

  10. VPLIV UPORABE E-GRADIV NA USPEH UČENCEV PRI PREDMETU GEOGRAFIJA

    OpenAIRE

    Škof, Iztok

    2013-01-01

    S spremembami na različnih znanstvenih, kulturnih in socialnih področjih so se spremembe pojavile tudi v izobraževalnih sistemih in njihovih glavnih elementih, kot so izobraževalni cilji, metode poučevanja, načini ocenjevanja, in v učnih načrtih. Danes v izobraževalnih ustanovah poučevanje-učenje ne sme biti omejeno na učbenike oz. učitelje, ampak moramo omogočiti vseobsegajoče vseživljenjsko izobraževanje. E-učenje to omogoča. Namen magistrske naloge je raziskati vpliv e-gradiv, dostop...

  11. Terapija s pomočjo konja pri motnjah avtističnega spektra

    OpenAIRE

    Purgaj, Natalija

    2014-01-01

    Motnja avtističnega spektra je biološko utemeljena, pervazivna razvojna motnja v različno težkih oblikah. Prisotno je nenormalno ali moteno vedenje, ki se kaže pred tretjim letom starosti. Težave so značilne na socialnem področju, v interakciji, komunikaciji, pojavlja se utesnjeno, stereotipno, ponavljajoče se vedenje. Prisotne so lahko tudi druge nespecifične težave. Motnja avtističnega spektra ni ozdravljiva, lahko pa se blažijo njeni simptomi z raznimi edukacijami, modifikacijskimi vedenji...

  12. PRI: transport and distribution networks of the electric power. Research program 2002/2003 (one year); PRI: Reseaux de transport de et de distribution de l'energie electrique. Programme de recherche 2002 / 2003 (1 an)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    This program proposes two actions. The first one deals with the energy transfers in networks. The study presents the control of networks in the new context of the deregulation which will imply a massive insertion of a decentralized production and the research of new tools of networks simulation using the new analysis methods by order reduction and fractals. The second one is devoted to the high voltage electronic and the energy transfer. (A.L.B.)

  13. 对基于PRI谱计算脉冲重复间隔的一种改进方法%An improved method of estimating PRI based on PRI spectrums

    Institute of Scientific and Technical Information of China (English)

    王文光; 常伟光; 王俊

    2006-01-01

    通过PRI变换可以得到PRI谱图,基于PRI谱估计脉冲重复间隔是一种有效的方法,具有很广的应用范围,但也有其局限性.本文针对PRI谱方法对参差雷达的PRI估计效果不理想的情况,结合脉冲列的自相关和一阶直方统计给出了一种改进方法.这种改进方法不仅保留了PRI谱方法的优点,对参差雷达也得到了很好的估计结果.

  14. Performance Analysis of PRI Transform in theRadar Pulse Signal's PRI Detection%PRI变换对脉冲雷达信号PRI检测的性能分析

    Institute of Scientific and Technical Information of China (English)

    安振; 李运祯

    2007-01-01

    传统的雷达脉冲列去交错算法很容易出现信号的脉冲重复间隔及其整数倍(即子谐波)同时存在的现象,给雷达脉冲串的去交错造成了困难.介绍了PRI变换的脉冲重复间隔估计算法,该算法几乎完全抑制了出现在自相关函数中的子谐波,同时研究了该算法对稳定重频、抖动重频、参差重频、滑变重频等各类信号的PRI检测性能.计算机仿真结果表明,这种算法适用性较强.

  15. NAPAČNA RAZUMEVANJA PREPROSTIH NARAVOSLOVNIH POJMOV PRI VZGOJITELJICAH IN ŠTUDENTIH PREDŠOLSKE VZGOJE

    OpenAIRE

    Breznik, Nika; Božič, Manja

    2014-01-01

    Pojem je miselna tvorba, ki je določena z bistvenimi lastnostmi in značilnostmi. Pojme razvijamo skozi proces učenja, ki traja celo življenje, nanj pa vplivajo različni dejavniki. Pojme razvijamo postopoma in prilagojeno miselnemu razvoju posameznika. Diplomsko delo govori o napačnem razumevanju preprostih naravoslovnih pojmov. Diplomsko delo je sestavljeno iz teoretičnega in empiričnega dela. V prvem delu smo se osredotočili na kognitivni razvoj otroka, učenje in razvoj pojmov. Posebno pozor...

  16. Limited access to HIV prevention in French prisons (ANRS PRI2DE: implications for public health and drug policy

    Directory of Open Access Journals (Sweden)

    Blanche Jerôme

    2011-05-01

    Full Text Available Abstract Background Overpopulation, poor hygiene and disease prevention conditions in prisons are major structural determinants of increased infectious risk within prison settings but evidence-based national and WHO guidelines provide clear indications on how to reduce this risk. We sought to estimate the level of infectious risk by measuring how French prisons adhere to national and WHO guidelines. Methods A nationwide survey targeting the heads of medical (all French prisons and psychiatric (26 French prisons units was conducted using a postal questionnaire and a phone interview mainly focusing on access to prevention interventions, i.e. bleach, opioid substitution treatment (OST, HBV vaccination and post-exposure prophylaxis (PEP for French prisoners. Two scores were built reflecting adherence to national and WHO international guidelines, ranging from 0 (no adherence to 10 (maximum adherence and 0 to 9 respectively. Results A majority (N = 113 (66% of the 171 prisons answered the questionnaires, representing 74% coverage (46,786 prisoners of the French prison population: 108 were medical units and 12 were psychiatric units. Inmate access to prevention was poor. The median[IQR] score measuring adherence to national guidelines was quite low (4.5[2.5; 5.5] but adherence to WHO guidelines was even lower 2.5[1.5; 3.5]; PEP was absent despite reported risky practices. Unsuitable OST delivery practices were frequently observed. Conclusions A wide gap exists between HIV prevention policies and their application in prisons. Similar assessments in other countries may be needed to guide a global policy reform in prison settings. Adequate funding together with innovative interventions able to remove structural and ideological barriers to HIV prevention are now needed to motivate those in charge of prison health, to improve their working environment and to relieve French prisoners from their currently debilitating conditions.

  17. Diurnal Patterns of Photosynthesis, Chlorophyll Fluorescence, and PRI to Evaluate Water Stress in the Invasive Species, Elaeagnus umbellata Thunb

    Science.gov (United States)

    2010-01-01

    effective quantum yield of pho- tosystem II: DF=F0m ¼ F0m Fs =F0m 238 Trees (2010) 24:237–245 123 Leaf xylem pressure potentials (W) were...with ontogenic changes in water limited Chenopodium quinoa leaves. Photosynthetica 40:227–232 Yates ED, Levia DP Jr, Williams CL (2004) Recruitment of

  18. Kritična analiza diskurza pri tolmačenju za skupnost v Sloveniji: doktorska disertacija:

    OpenAIRE

    2015-01-01

    The thesis explores community interpreting in Slovenia with special regard to the specifics of interpreter-mediated interaction and the role of interpreting in institutional settings. The doctoral project aims to present the field and show the significance of interpreters' work, their professional competence and the understanding of this process in facilitating successful communication. The study argues that: a) interpreting and institutional discourse influence each other reciprocally; b) in...

  19. Hidrokinezioterapija v primerjavi s kinezioterapijo pri starejših osebah po vstavitvi totalne endoproteze kolčnega sklepa

    OpenAIRE

    Jakovljević, Miroljub; Vauhnik, Renata

    2015-01-01

    Background: Aquatic therapy allows secure, active exercise with pain reduction using a combination of the water's buoyancy, hydrostatic pressure resistance and warmth. By aquatic therapy, elderly patients after total hip replacement can achieve more positive effects than by land-based exercise. The aim of the study was to investigate the use of aquatic-based exercises in the rehabilitation programme after a hip fracture surgery in elderly adults. Results: Both groups, regardless of the type o...

  20. PRI-CAT: a web-tool for the analysis, storage and visualization of plant ChIP-seq experiments.

    NARCIS (Netherlands)

    Muino, J.M.; Hoogstraat, M.; Ham, van R.C.H.J.; Dijk, van A.D.J.

    2011-01-01

    Although several tools for the analysis of ChIP-seq data have been published recently, there is a growing demand, in particular in the plant research community, for computational resources with which such data can be processed, analyzed, stored, visualized and integrated within a single, user-friend

  1. Vloga in pomen ustvarjalnega pisanja pri pouku književnosti v osnovni šoli: doktorska disertacija:

    OpenAIRE

    Blažić, Milena Mileva

    2000-01-01

    The thesis presents the meaning and role of creative writing in teaching literature in elementary school. The first theoretical part discusses contemporary cognitive theory in which writing itself is a process as important as the text. Since the traditional conception of writing was centered on the result of the writing process – the text. The cognitive writing model is composed of three parts: 1. task environment; 2.the writers’ long term memory and 3. writing process. In addition two writin...

  2. Statistical methods for processing seismic information and studying complicated media. Statisticheskiye metody obrabotki seysmicheskoy informatsii pri issledovanii slozhnykh sred

    Energy Technology Data Exchange (ETDEWEB)

    Troyan, V.N.

    1982-01-01

    For the first time materials are generalized concerning statistical methods of processing seismic information which are used more widely in prospecting minerals (oil, gas, ore) in regions of complex structure and great depths. The methods provide reliable identification of useful signals in the background of interferences. Fundamentals are examined of methods of constructing algorithms and programs used in interpretation, and their efficiency.

  3. Izbrani kazalci obremenjenosti okolja pri študijah ranljivosti okolja = Selected environmental pollution indicators in environmental vulnerability studies

    Directory of Open Access Journals (Sweden)

    Dejan Cigale

    2002-01-01

    Full Text Available One of the components of the environmentalvulnerability studies is also the evaluation of the pollution of theenvironment. In the article, the analysis of the selected indicators of theachieved degree of the total and integral pollution is presented. In theframework of the environmental vulnerability studies on the spatial levelof landscape-ecological types for this purpose the following indicatorswere selected: population density, density of working places, trafficintensity and percentage of forests.

  4. Vloga finančnih omejitev pri poslovanju na tujih trgih = The Role of Financial Constraints on Foreign Markets

    Directory of Open Access Journals (Sweden)

    Crt Kostevc

    2006-12-01

    Full Text Available The Role of Financial Constraints on Foreign MarketsAbstract: Recent research on the role of foreign activities and their impact on firm characteristics has, with few exceptions, confirmed the existence of persistently large differences between exporters and non-exporters in terms of size, investments, innovative activity etc. In spite of the mounting evidence on the advantages of exporters over firms focused solely on their domestic markets, the source of these differences is yet to be explained. The present contribution attempts to analyze the role of financial constraints as one of the factors determining which firms will export and which will not. Through a survey of the existing literature, the role of financial constraints that limit foreign market access to only a subset of the firms, will be revealed. It is shown that financial constraints – even when other factors are explicitly considered (such as firm size, productivity, capital intensity, . . . – to a large extent determine the firms that will be able to enter into foreign markets, and also mean that financially constrained firms end up exporting less frequently and in smaller quantities than could otherwise be expected.

  5. The Effect o f Twelve - Week Recreation Act i v ities on the Anxiety Level of Female Pri soners

    Directory of Open Access Journals (Sweden)

    Zekiye B AŞARAN

    2015-07-01

    Full Text Available T h e a i m o f t h e s t u d y w a s to examine the effect of twelve - weeks recreation activities on the trait anxiety level of female prisoners in the prisons. The sampling of this study consists of 45 female prisoners who are in the Open Prison in Kandira, 22 of whom are in the experimental group and 23 of whom are in the control group. Different activities, such as music, dance, meditatio n, sportive activities, movies and videos, fun and entertainment activities and competitions, were performed one and half hours a day and two days a week. This lasted for twelve weeks. The data were collected by pre - tests and post - tests that were given bo th at the beginning and at the end of this program. Personal information form and Spielberger anxiety inventory were used as the data collecting tool. The reliability coefficient (Cronbach Alpha was calculated as ,873. The data were analysed with SPSS Win dows 18 programme. Descriptive statistics and Wilcoxon test were conducted. A si gnificant statistical correlation was determined between the prisoners’ pre - tests and post - tests scores in the experimental group (p <0.05. A s c o n c l u s i o n , a positive impact on trait anxiety levels of prisoners w a s f o u n d a f t e r 12 weeks o f recreational activities.

  6. Implementacija sistemskih organizacijskih postavitev pri ustanovitvi računovodskega podjetja Aktiva 3000 d.o.o.

    OpenAIRE

    2016-01-01

    Drznost in inovativnost nam dajeta moč, da raziščemo in prikažemo kako lahko z metodo organizacijske sistemske postavitve dobimo nove, sveže uvide v procesu ustanavljanja računovodskega podjetja. Vsak organizacijski sistem se začne z idejo, namenom, cilji, poslanstvom in vizijo. Predstavljamo svojo idejo, kaj delati, kako delati, postavili smo si cilje, preverili svoje zmožnosti, analizirali konkurenco, analizirali, kako deluje trg, analizirali smo vsako podrobnost, ki vpliva na uspešn...

  7. Kalibrowka 900- kanalxnogowremqproletnogo detektoraiizmereniespektrowadronow wowzaimodejstwiqhqder pb+pb pri “nergii 158 g“w / nuklon

    CERN Document Server

    Kolesnikov, V I

    This paper explains in detail the layout and characteristics of TOF-L detector (part of NA49 and NA61 experiments), calibration procedure used, as well as application of acquired information in analysis of collisions of lead-lead nuclei

  8. PROJECTION OF PENETRATION OF THE BROKER IN HOME BRAZILIAN CAPITAL MARKET THROUGH LINEAR, GOMPERTZ AND FISHER – PRY MODELS

    Directory of Open Access Journals (Sweden)

    Carlos Kawamoto

    2010-09-01

    Full Text Available This paper aims to discuss the theories of internationalization and competitiveness, the positioning of a Brazilian company from agribusiness industry, exporting meat and food that has become a major global player in this industry JBS - Friboi. This company has been internationalized very fast in recent years and become a global leader in beef production, the second to the production of chicken meat and third largest producer of pork in the U.S. (JBS, 2010. The case is emblematic of JBS when analyzed under the concept of Porter's diamond applied to Brazil. This company gained scale and competitiveness with the access to natural resources of prodution (condition factor, but now it is necessary for maintenance its leading position, it requires a strategy grounded in innovation beyond just scale and operational excellence.

  9. Viga on öelda, et me ei vaja PRi / Roger Hayes, Alasdair Sutherland ; interv. Tiiu Värbu

    Index Scriptorium Estoniae

    Hayes, Roger

    2001-01-01

    Eesti Suhtekorraldajate Liidu 5. aastapäeva seminaril osalevad Rahvusvahelise Kommunikatsiooni Instituudi direktor R. Hayes ja maailma ühe juhtiva suhtekorraldusfirma Manning, Selvage & Lee asepresident A. Sutherland

  10. A Multimethod Assessment of Juvenile Psychopathy: Comparing the Predictive Utility of the PCL:YV, YPI, and NEO PRI

    Science.gov (United States)

    Cauffman, Elizabeth; Kimonis, Eva R.; Dmitrieva, Julia; Monahan, Kathryn C.

    2009-01-01

    The current study compares 3 distinct approaches for measuring juvenile psychopathy and their utility for predicting short- and long-term recidivism among a sample of 1,170 serious male juvenile offenders. The assessment approaches compared a clinical interview method (the Psychopathy Checklist: Youth Version [PCL:YV]; Forth, Kosson, & Hare,…

  11. [Reactivity of the physiological system of the connective tissue and immunoglobulin levels in divers of the Arctic Pri-Elbrus regions].

    Science.gov (United States)

    Pavlovich, S I

    1991-01-01

    The functional state of the conjunctive tissue system in deep-divers has been evaluated by the Kavetsky-Leshchinsky method. The content of different classes of immunoglobulins in the mucosa of upper respiratory tracts has been studied by the fluorescent antibody method under conditions of the Arctic and highland. The long-term divings cause a decrease in the reactivity of a physiological system of conjunctive tissue and a shift in the parameters of the immunological organism status. These changes are found to be more pronounced in the deep divers with underwater work experience of more than 2,000 hours. The general immunological reactivity of the organism rises significantly after 2.5 months stay under conditions of the Elbrus (height--2100 m). These facts confirm an idea that favourable climatic factors of the highland are expedient to be used in the complex of rehabilitation measures for deep-divers.

  12. Vrednovanje Dobara pri Ocjeni Društvene Opravdanosti Investicionih Projekata / Evaluation of Goods in Assessment of Social Justification of Investment Projects

    Directory of Open Access Journals (Sweden)

    Čoprka Miodrag

    2015-06-01

    Full Text Available The effects of an investment, depending on their type and size, may manifest differently on the economy, which is why it is necessary to examine various aspects of their evaluation. Starting from the fact that the market cannot always and at all times and circumstances properly evaluate all the goods and services from the society viewpoint, commercial justification may greatly differ from social justification. The main starting point in the process is the application of billing prices instead of market prices, i.e. consideration of opportunity costs and benefits of investment projects, as they are an integral part of the investment and are not applied in the best alternative use. Starting from the shortcomings of conventional financial analyses in the identification and evaluation of the effects of investment projects from a social aspect, it is necessary to promote increasingly popular and relevant approaches.

  13. Napoved razvoja hiperbilirubinemije pri donošenih novorojenčkih z neinvazivnimi metodami: Prediction of hyperbilirubinemia by noninvasive methods in full-term newborns:

    OpenAIRE

    Bratanič, Borut; Pavlin, Tatjana; Gradecki, Mirjam; Furlan, Danijela; Žalec, Lidija; Oštir Mevželj, Darinka

    2013-01-01

    Introduction: The noninvasive screening methods for bilirubin determination were studied prospectively in a group of full-term healthy newborns with the aim of early prediction of pathological neonatal hyperbilirubinemia. Laboratory determination of bilirubin (Jendrassik- Grof (JG)) was compared to the noninvasive transcutaneous bilirubin (TcBIL) together with the determination of bilirubin in cord blood. Methods: The study group consisted of 284 full-term healthy consecutively born infants i...

  14. Razlike med spoloma pri nekaterih simptomih stresa ter intenzivnost doživljanja stresnih simptomov = Gender Differences in Some Stress Symptoms and Intensity of Experiencing Stress Symptoms

    Directory of Open Access Journals (Sweden)

    Maja Meško

    2010-06-01

    Full Text Available The aim of this study was to identify the symptoms of stress and to establishgender differences in stress symptoms. We tried to find out ifthere are gender differences in the stress level at work, if there aregender differences in terms of stress symptoms frequency, and if thereare gender differences in terms of the intensity of experiencing stresssymptoms. In this study 85 randomly selected managers from Slovenecompanies participated. The results of the study have shown that thereare gender differences in terms of all the above mentioned factors,namely a statistically higher level of stress in women. In terms of stresssymptoms occurrence there are gender differences in some stress symptomsas well as in the intensity of experiencing stress symptoms.

  15. A Brief Discussion on Learning Style Construction in Pri-vate Higher Vocational Colleges%浅谈民办高职院校学风建设

    Institute of Scientific and Technical Information of China (English)

    顾小移

    2014-01-01

    The quality of learning style construction in higher vo-cational colleges is not only directly related to the survival and development of private colleges, but also related to higher voca-tional college students' vital interests. Combined with the current situation of private higher vocational colleges at the present stage, in view of the problems in learning style construction, the writer proposed personal views, hoping to find out more effective meth-ods and measures for enhancing learning style construction.%高职院校的学风建设的好坏不仅直接关系着民办高校自身的生存和发展,更是一件关系着高职院校学生切身利益的大事情。本文结合现阶段民办高职院校的校情,针对学风建设中存在的问题,提出自己的观点和看法,以期找到加强学风建设更有效的方法和措施。

  16. Španjolski stručnjaci u Ciudad de Méxicu: priče o radu i tržištu rada

    OpenAIRE

    Mendoza, Cristóbal; Ortiz Guitart, Anna

    2008-01-01

    U radu se istražuju radna iskustva skupine kvalificiranih španjolskih migranata u Ciudad de Méxicu uporabom kvalitativnih podataka. Među intervjuiranim Španjolcima identificiraju se dva tipa migranata: premještenici unutar TNC-a (transnacionalnih korporacija) i »migranti u sredini« (»oni koji nisu ni niskokvalificirani ni visokokvalificirani migranti«; Conradson i Latham, 2005a). To donekle dovodi u pitanje pretpostavke o kvalificiranim radnicima pokazujući mnogostruke radne putanje te iskust...

  17. Izboljšanje javne participacije pri prostorskem načrtovanju s pomočjo orodij spleta 2.0

    Directory of Open Access Journals (Sweden)

    Igor Bizjak

    2012-01-01

    Full Text Available Javna participacija (ang. public participation je ena od oblik sodelovanja oziroma udeleževanja javnosti v postopkih prostorskega načrtovanja, ki omogoča posameznikom in skupinam, da izrazijo svoja mnenja, dajejo pobude in aktivno sodelujejo v teh postopkih. Ker je javna participacija pogosto omejena le na dajanje pripomb na že pripravljene prostorske načrte, predvidevamo, da bi jo lahko izboljšali s pomočjo informacijskih tehnologij, in sicer z uporabo novih spletnih orodij. Ena takih novosti je splet 2.0 (ang. web 2.0, koncept, ki je bil prvič predstavljen v letu 2004 in je v svetovni splet prinesel veliko orodij, ki jih koristno uporabljajo socialna omrežja in različne spletne storitve kot participatorne storitve z velikim številom uporabnikov. V članku so analizirane možnosti za medsebojno interakcijo javne participacije in spleta 2.0. Predstavljene so zahteve javne participacije v postopkih prostorskega načrtovanja, osnovna orodja spleta 2.0 in njegove zmožnosti glede omogočanja participacije v procesih prostorskega načrtovanja. V prispevku je ugotovljeno, da so orodja spleta 2.0 zelo uporabna za vključevanje javnosti v procese prostorskega načrtovanja, vendar zahtevajo veliko predpriprav in dobro tehnično osnovo.

  18. Zdravljenje s tamsulozinom pri kamnih v spodnji tretjini sečevoda: Treatment with tamsulosin in patients with distal ureteral calculi:

    OpenAIRE

    Bizjak, Igor; Erklavec, Marko; Jagodič, Klemen; Korošec, Helena; Poteko, Sandi

    2008-01-01

    Background Symptomatic ureteral calculi are one of the most important issues in urologist emergency clinical settings. Spontaneous passage of distal ureteral calculi is usually achieved with good hydration and spasmoanalgetic drugs. á-blocker therapy may facilitate and accelerate the spontaneous passageof ureteral stones.Patients and In retrospective study we analyzed patients with renal colic admitted to our department in methods 2006. All of them had clinical examination, urine and blood an...

  19. Pričakovanja študentov glede na razlike v kakovosti storitev visokošolskih zavodov = Student Expectations of Higher Education Institution Services

    Directory of Open Access Journals (Sweden)

    Marijana Sikošek

    2011-09-01

    Full Text Available New challenges witnessed also in Slovenian higher education requirea different approach towards understanding students and their expectations,as higher education becomes increasingly market-oriented.Since research on assessing student expectations is still rare, we havedecided to establish the expectations of students regarding their studiesat the University of Primorska. With the help of factor analysis, wehave determined that these expectations are related to the followingsix factors: relationship between teaching staff and students, faculty’sreputation, faculty’s environment, employment chances, academic excellence,and staff competence and expertise. Cluster analysis has revealeda difference between the expectations of students of professionalstudy programmes and students of academic study programmes.

  20. Sindrom pomanjkanja rastnega hormona pri odraslem - učinki nadomestnega zdravljenja: Syndrome of growth hormone deficiency in adults - effects of growth hormone replacement therapy:

    OpenAIRE

    Pfeifer, Marija

    2001-01-01

    Background. After the cessation of longitudinal growth, growth hormone (GH) continues to subserve an important role in the regulation of body metabolism (stimulation of lipolysis and lipid oxidation, protein synthesis, insulin antagonism, and sodium and water retention) to optimise body composition and function. Most patients with hypopituitarism exhibit the syndrome of GH deficiency with a number of abnormal features which can be reversed with recombinant GH replacement therapy. Conclusions....

  1. Sodelovanje med zdravniki družinske medicine in specialisti psihiatri pri zdravljenju bolnikov z depresijo: Cooperation between family practitioners and psychiatrist in treating patients with depression:

    OpenAIRE

    2011-01-01

    Background: By 2015, mental illness will become the greatest healthcare burdenin the world. Within the community, people with depression are most often treated by family doctors. Treatment for depression also includes psychiatric specialists, with varying cooperation between the primary care provider and the psychiatrist. This studyćs goal was to define cooperation between family doctors and psychiatric specialists when treating patients withdepression. Methods: In 2009 six focus groups were ...

  2. Správanie spotrebitel'a pri nákupe mliečnych produktov v maloobchodnom reťazci Tesco

    OpenAIRE

    Moravčíková, Mária

    2015-01-01

    Consumer behavior buying milk product in string of retail Tesco. Bachelor thesis. Brno: Mendel University, 2015. The bachelor thesis is focused on in store comunication, consumer´s behaviour during shopping for milk and milk products in a Tesco shop. It is based on factors, which influence the consumers during purchasing, such as price, packaging and sales promotion. Furthermore it is dealing with visual merchandising and its influence on behaviour of consumers. Personal questionnaire, which...

  3. Pomen Centra šolskih in obšolskih dejavnosti (CŠOD pri pouku in terenskem delu geografije = Geography in Slovenian Field Study Centres

    Directory of Open Access Journals (Sweden)

    Špela Odar

    1998-01-01

    Full Text Available The paper represents a program written for a "week in nature" for students of 5th, 6th and 7th primary school classes. Primary stress is given to the geographic themes, field work and theory, which are presented in a weekly course in one of 13 field studies centres in Slovenia.

  4. Metodología en operaciones de cementación priMaría y forzada utilizando nuevas tecnologías

    OpenAIRE

    Campos, Isrrael; Armas, Diego; Guecara, Dani; Vargas, Javier; Tapia, Daniel

    2011-01-01

    El presente trabajo tiene como finalidad mostrar el diseño de la cementación del pozo arcángel 1D, En la primera parte planteamos brevemente, los diferentes tipos de Cementaciones que son aplicadas en la terminación de una fase en un pozo petrolero, así como, herramientas usadas, técnicas aplicadas y el uso de diferentes tipos de revestidores según la profundidad alcanzada en cada sección. Luego se presentara una descripción litológica de la zona en la cual se ha planificado la pe...

  5. Priones: una revisión de la enfermedad debilitante crónica y su posible control anti-priónico

    OpenAIRE

    Ricardo Millán-González; Diego Soler-Tovar

    2014-01-01

    Los priones son proteínas del sistema nervioso, que cuando sufren cambios en su estructura protéica son responsables de generar enfermedades neurodegenerativas. Existe un gran interés por la alta estabilidad de estas moléculas. Estudios señalan que poseen una alta resistencia a ser inactivadas por varios agentes y que pueden persistir en la tierra años después de ser enterradas, generando una reserva de infectividad ambiental. En la medicina veterinaria existen varios animales domésticos susc...

  6. A method for formalizing the description of structures of porous mediums with their automatic analysis. Metod formalizovannogo opisaniya struktur poristykh sred pri ikh avtomaticheskom analize

    Energy Technology Data Exchange (ETDEWEB)

    Kostenko, I.F.

    1983-01-01

    A set of parameters is examined which characterizes the properties of the sum of geometrical objects which make up the structure of the pore space of oil and gas collector rocks. The properties of the structures such as the mutual disposition of individual objects, the relationships of their dimensions, the complexity of form and the presence of the predominate orientation of the objects in the plane are also evaluated. The possibility of using the measured parameters for classifying structures is shown.

  7. Fault Analysis of PRI Interface Testing in GSM-R%GSM-R网络中PRI接口测试的故障分析

    Institute of Scientific and Technical Information of China (English)

    苗丽丽; 蒋文怡; 贾怀义

    2005-01-01

    主要根据国际ITU-T标准及原国家邮电部技术规定,结合实验室监视到的PRI接口消息,深入分析了GSM-R网络中PRI接口测试时出现的各种异常现象,并给出改进建议.

  8. 氯化亚锡对胶乳标准胶PRI值的影响%Effect of stannous chloride on the PRI of standard rubber

    Institute of Scientific and Technical Information of China (English)

    冯玉英

    2004-01-01

    介绍了氯化亚锡在不同季节对胶乳标准胶P0、PRI值的影响.结果表明,在3~4月它对胶乳标准胶的P0、PRI值影响不大;在5~10月的高温季节会造成胶乳标准胶P0、PRI值下降,甚至不达标;在生产上应严格控制氯化亚锡的使用.

  9. Parcels and Land Ownership, k7200pri - Parowan, Published in 2005, 1:24000 (1in=2000ft) scale, Iron County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset, published at 1:24000 (1in=2000ft) scale, was produced all or in part from Other information as of 2005. It is described as...

  10. Identification of Radar PRI Modulation Style Characteristic Curve%雷达PRI调制样式特性曲线的识别

    Institute of Scientific and Technical Information of China (English)

    吴振强; 张国毅; 常硕

    2015-01-01

    针对PRI调制样式特性曲线的特点提取了其最大值、等值比以及连续性参数作为特征参数,对PRI调制样式进行了分类,分析了非调制参数和调制参数对特性曲线和特征值的影响,利用支持向量机验证了存在脉冲丢失等噪声的情况下利用本研究提取的特征参数识别PRI调制样式的有效性.

  11. 基于PRI变换的未知雷达信号分选算法研究%Research on PRI Transform Algorithm of Unknown Radar Signal

    Institute of Scientific and Technical Information of China (English)

    王宇

    2010-01-01

    针对未知雷达信号主处理中脉冲重复间隔的精确分选部分,介绍了PRI变换的脉冲重复间隔估计算法,提出针对固定重频、抖动重频和参差重频信号的PRI变换算法的仿真和改进方法.

  12. Portable infrared camera as a tool in topoclimatic research = Prenosna infrardeča kamera kot pripomoček pri topoklimatskem raziskovanju

    Directory of Open Access Journals (Sweden)

    Miroslav Vysoudil

    2009-01-01

    Full Text Available The paper presents some possibilities of application of a portable infrared camera fortopoclimatic researches, especially for establishing the differences in temperatures of theso-called active surface layer. Demonstrated is the dependence of surface temperature onsurface characteristics, such as land use, morphological features and the time of the day. Theresults can be useful for establishing topoclimatic units with similar temperature regimes.

  13. Medosebni odnosi in vsebina dela kot dejavnika stresa pri srednjem managementu = Interpersonal Relationships and Work Content as Stress Factors among Middle Management

    Directory of Open Access Journals (Sweden)

    Polona Kožuh

    2011-06-01

    Full Text Available In the modern society, where importance is placed on progress, newtechnologies and economic trends, there is a lack of awareness of thesignificance of good interpersonal relations and organizational culturewithin a company. They both contribute to the better functioningof employees as well as the entire organization. The purpose of thisstudy was to determine any possible differences between the stressorslinked to the relationships within an organization and those linked tothe work of middle management. The study included 58 middle managersworking in commercial activity in the Ljubljana region. The resultshave indicated that the amount of stressors related to the relationships,and stressors related to the managers’ work, that affect themiddle managers participating in the survey is on average the same.The results have therefore shown no statistically significant differencebetween the two forms of the studied stress factors.

  14. 一种改进型PRI变换雷达信号分选方法%De-interleaving of Radar Signal Based on Improved PRI Transform Algorithm

    Institute of Scientific and Technical Information of China (English)

    田芬芳

    2010-01-01

    基于PRI分选有多种方法,其中PRI变换法对脉冲重复间隔有较高的估计精度,并克服了传统直方图统计法中的子谐波问题.文中在对PRI变换法进行深入研究的基础上,提出了一种改进的自适应PRI变换法,仿真结果验证了新算法的可靠性.

  15. Parcels and Land Ownership, k2400pri - Parowan, Published in 2005, 1:24000 (1in=2000ft) scale, Iron County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset, published at 1:24000 (1in=2000ft) scale, was produced all or in part from Other information as of 2005. It is described as...

  16. Discussion of network construction based on ISDN PRI interface of Meridian%Meridian交换机使用ISDN PRI接口组网

    Institute of Scientific and Technical Information of China (English)

    钟馥遥; 王金元

    2002-01-01

    使用PRI接口作局间中继进行组网,具有传统随路信令的数字中继所不可比拟的优越性和强大功能,该文简要介绍PRI接口的组网方式、网络功能及安装调试过程。

  17. An SNP site in pri-miR-124, a brain expressed miRNA gene, no contribution to mesial temporal lobe epilepsy in an Italian sample.

    Science.gov (United States)

    Manna, Ida; Labate, Angelo; Borzì, Giuseppe; Mumoli, Laura; Cavalli, Salvatore Maria; Sturniolo, Miriam; Quattrone, Aldo; Gambardella, Antonio

    2016-08-01

    Mesial temporal lobe epilepsy (MTLE) is the most common type of refractory epilepsy and is usually associated with hippocampal sclerosis (Hs). The pathogenesis of MTLE involves many biological pathways, some of which seem to be regulated by microRNAs (miRNAs). Increasing evidence shows that single nucleotide polymorphisms (SNPs) or mutations in miRNAs sequence may affect the processing and function of miRNAs and participate in the occurrence of diseases. In this study, the effect of the SNP of one neuronal miRNA, miR-124, on susceptibility to MTLE was investigated using a case control study. To understand the role, a common C/G polymorphism designated rs531564 in the molecular mechanisms of MTLE, we sought to determine whether this genetic variant could influence susceptibility to disease in a cohort of 307 MTLE patients and 306 healthy controls, using TaqMan allelic discrimination assay, on an Applied Biosystems PCR platform. No statistically significant differences were found in the allele or genotype distributions of the miR-124 rs531564 polymorphism among MTLE patients and MTLE-free control subjects (p > 0.05). Our results demonstrate that this SNP has no major role in genetic susceptibility to MTLE, at least in the population studied here.

  18. Možnost ponovne uporabe regeneracijske odpadne vode pri ionskem izmenjevalcu za mehčanje vode v Pivovarni Laško

    OpenAIRE

    Nemec, Nina

    2016-01-01

    Pivovarska industrija je en izmed velikih porabnikov vode, ki hkrati proizvaja tudi velike količine industrijske odpadne vode, ki obremenjujejo ekosistem. Teh težav se zavedajo tudi v podjetju Pivovarna Laško, ki kot IPPC (Integrated Pollution Prevention and Control – celovito preprečevanje in nadzor onesnaževanja) zavezanec nenehno optimizira uporabo vode v proizvodnem procesu, hkrati pa skrbi za ustrezno ravnanje z odpadnimi vodami. V magistrskem delu smo raziskali možnost ponovne uporabe r...

  19. 小学语文教学重在“悟”%Nothing is more Important than “Comprehension” in Pri-mary School Chinese Language Teaching

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    Primary school Chinese language teaching shoulders the responsibility of cultivating students' reading habit, and a good reading habit can even influence the development of a per-son's personality. Making pupils love reading is the responsibility of every Chinese language teacher. Teachers should guide stu-dents and give them methods, so as to help them comprehend e-motions in reading.%  小学语文教学承担着培养学生良好阅读习惯的重任,而良好的阅读习惯甚至会关系到一个人健康人格的发展。让学生从小就爱上读书,每一位语文老师责无旁贷。老师要善于引导学生,给出方法,读中悟情、入境悟美、激疑悟新。

  20. Expected service quality measurement in tourism higher education: Merjenje pričakovane kakovosti storitev visokega izobraževanja v turizmu:

    OpenAIRE

    2006-01-01

    This paper starts with background theory and the outlines the results of an exploratory study conducted on students at the Faculty of Tourism and Hospitality Management in Opatija (Croatia). The aims of the study are to: (1) provide a brief review of the existing tools for measuring students' experience, (2) assess students' expectations of service quality in tourism higher education, (3) test the reliability of a modified SERVQUAL scale, (5) establish the number of dimensions of service qual...

  1. Organochlorines and trace elements in avian pry of peregrine falcons in Colorado: Ten years after: First year interim report of a two year study

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Bird species considered potential prey for peregrine falcons were collected at 10 sites in Colorado for analysis of organochlorine (OC) compounds and trace elements...

  2. 论小学语文对话教学的有效策略%On the Effective Strategies of Dialogue Teaching of Pri-mary School Chinese

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

      通过对对话教学概念的梳理,提出了对话教学是强调了师生之间的平等、民主、尊重、信任,体现了教学的主体性、创造性、人性化,以文本为参照来促进师生共同发展的一种现代教学活动;从三个方面探讨了小学语文对话教学的有效策略:构建以人为本的新型师生观,践行教师主导和学生主体有机结合的对话教学,倡导生成性、开放性的对话教学。%Through sorting concept of dialogue teaching, the new concept of dialogue teaching has been come up with in this re-search which emphasizes equal and democracy and respect and trust of teacher and student's relationship and which embodies main body and creativity and humanity of teaching and which is a modern teaching activity of improving teacher and student' co-development by means of Chinese textbook. The effective strategies of dialogue teaching of primary school Chinese has been discussed from three aspects of constructing new teacher and student' relationship based on human rights and practicing dialogue teaching of teacher and student' double main body in-quiring dialogue teaching of growing and opening.

  3. Pressure induced structural phase transition of PrX, PrY (X = S, Se, Te) chalcogenides and (Y = N, P, As) pnictides

    Science.gov (United States)

    Varshney, Dinesh; Shriya, Swarna; Dube, A.; Varshney, Meenu

    2012-06-01

    Pressure induced structural aspects of NaCl-type (B1) to CsCl-type (B2) structure in Praseodymium pnictides and chalcogenides are presented. An effective interionic interaction potential with long range Coulomb, van der Waals interaction and the short-range repulsive interaction upto second-neighbor ions within the Hafemeister and Flygare approach with modified ionic charge is developed. Deduced results on volume discontinuity in pressure volume phase diagram identify the structural phase transition from B1 to B2 structure consistent with the known results.

  4. Association of Polymorphisms in three pri-miRNAs that Target Pepsinogen C with the Risk and Prognosis of Gastric Cancer

    Science.gov (United States)

    Wu, Ye-feng; Xu, Qian; He, Cai-yun; Li, Ying; Liu, Jing-wei; Deng, Na; Sun, Li-ping; Yuan, Yuan

    2017-01-01

    We aimed to explore the associations of polymorphisms in three microRNAs (miRNAs) (let-7e rs8111742, miR-365b rs121224 and miR-4795 rs1002765) that target PGC with the risk and prognosis of gastric cancer/atrophic gastritis. Sequenom’s MassArray was used to genotype the miRNA polymorphisms in 724 gastric cancer cases, 862 atrophic gastritis cases and 862 controls in a Chinese population. We found that let-7e rs8111742 and miR-4795 rs1002765 were associated with the risk of gastric cancer in the H. pylori-positive subgroup. MiR-365b rs121224 was associated with the risk of intestinal-type gastric cancer in the alcohol consumption subgroup. Intestinal-type gastric cancer patients at Borrmann stages III-IV who carry the miR-365b rs121224 GG genotype had better prognosis compared with those who carry the CG or CC genotypes. MiR-365b rs121224 was associated with Lauren typing and TNM staging, in which the distribution of GG genotype carriers in intestinal-type gastric cancer and the TNM stage I-II subgroup was higher than that of CG or CC genotypes, which contrasted with the distribution in diffuse-type gastric cancer or TNM III-IV groups. These findings suggested that the polymorphisms in these miRNAs might be biomarkers for gastric cancer risk and prognosis, especially for populations infected with Helicobacter pylori or who consume alcohol. PMID:28067243

  5. Multiple genetic pathways for restarting DNA replication forks in Escherichia coli K-12.

    OpenAIRE

    Sandler, S J

    2000-01-01

    In Escherichia coli, the primosome assembly proteins, PriA, PriB, PriC, DnaT, DnaC, DnaB, and DnaG, are thought to help to restart DNA replication forks at recombinational intermediates. Redundant functions between priB and priC and synthetic lethality between priA2::kan and rep3 mutations raise the possibility that there may be multiple pathways for restarting replication forks in vivo. Herein, it is shown that priA2::kan causes synthetic lethality when placed in combination with either Delt...

  6. PRI 8.1: CARNOT. Analysis and research community of the new orientations of the thermodynamics. Activity report june 2002/ june 2004; PRI 8.1: CARNOT. Communaute d'Analyse et de Recherche des Nouvelles Orientations de la Thermodynamique. Rapport d'activite juin 2002/ juin 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    This progress report takes stock on the CARNOT project. This project aims to standardize the different approaches or identify the application fields of each thermodynamical methodology. The cycles analysis and more generally thermodynamical machines (design, energy efficiency, new energy utilization concepts). The environmental impact is also an important topic of the project. (A.L.B.)

  7. Pri svete vetra : Tjomnõi tunnel; Kogda-to na jarmarke; Daljoki, zabõtõi...; 'Hriplõi...'; Grustnaja pessenka; Bõla bõ metla...; Kannel; 'Pri svete vetra...'; Odnazhdõ; 'Kipariss...'; 'Na starom kostrisht'e...'; 'Vot spjashtshaja ja...'; 'Ser�

    Index Scriptorium Estoniae

    Vaarandi, Debora, 1916-2007

    1996-01-01

    Orig.: Tume tunnel; Vana laadalugu; Kauge unustatud; "See on..."; Kurb lauluke; Luuata; Kandlemängija; "Tuule valgel..."; Üks; "Küpress..."; "Magan tormis mis mujal..."; "Ööliblikaid halle ja maokaid..."; "naised on need..."; "Krestomaatsed luuletused..."; Sisevaade

  8. GenBank blastx search result: AK061863 [KOME

    Lifescience Database Archive (English)

    Full Text Available ome 6q13-15 Contains the 3' part of the BCKDHB gene for branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease), complete sequence.|PRI PRI 2e-47 +2 ...

  9. GenBank blastx search result: AK105761 [KOME

    Lifescience Database Archive (English)

    Full Text Available ome 6q13-15 Contains the 3' part of the BCKDHB gene for branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease), complete sequence.|PRI PRI 1e-115 +2 ...

  10. GenBank blastx search result: AK104686 [KOME

    Lifescience Database Archive (English)

    Full Text Available ome 6q13-15 Contains the 3' part of the BCKDHB gene for branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease), complete sequence.|PRI PRI 1e-48 +1 ...

  11. GenBank blastx search result: AK060465 [KOME

    Lifescience Database Archive (English)

    Full Text Available osome 10 Contains the 3' end of a novel gene (FLJ37306), a novel gene and a ribosomal protein S27 (metallopanstimulin 1) (RPS27) pseudogene, complete sequence.|PRI PRI 1e-47 +1 ...

  12. GenBank blastx search result: AK110355 [KOME

    Lifescience Database Archive (English)

    Full Text Available osome 10 Contains the 3' end of a novel gene (FLJ37306), a novel gene and a ribosomal protein S27 (metallopanstimulin 1) (RPS27) pseudogene, complete sequence.|PRI PRI 5e-90 +3 ...

  13. Partopreninte la Unuan Azian Kongreson

    Institute of Scientific and Technical Information of China (English)

    UEDA Tomohiko

    1996-01-01

    Mi opinias,ke la kongresaj aranoj estisbonege faritaj pro la atentaj prizorgoj de KAEM(Komisiono de UEA pri Azia Esperanto-Mova-do).Mi iom skribos pri miaj personaj impresoj.Dum la kongreso mi estis relative diligenta

  14. A Brief Analysis on the Application of E-learning in Pri-mary and Secondary School Teachers Training%浅析E-learning在中小学教师培训中的应用

    Institute of Scientific and Technical Information of China (English)

    葛吉雪

    2016-01-01

    培训是提升教师的教育教学技能与实践创新能力的重要途径.要让e-learning在中小学教师培训中发挥作用,就必须充分认识e-learning在教师培训中的必要性、加强e-learning基础设施建设、拓展e-learning资源、以多元方式对参训教师进行激励与评价.%Training is an important way to improve teachers' edu-cation teaching skills and practice the innovation capability. If e-learning plays a role in primary and secondary school teachers training, we should fully recognize e-learning's necessity in teachers training, strengthen infrastructure construction and ex-pand resources of e-learning. And we also should stimulate and evaluate teachers with multiple ways.

  15. PBL模式对小学英语自我效能的影响%The Influence of PBL Model on the Self-efficacy of Pri-mary School English

    Institute of Scientific and Technical Information of China (English)

    何轶君

    2016-01-01

    自我效能是人们对自己能否成功完成某任务的主观判断,它与学业成就之间存在显著相关性.作为以学习者为中心的学习模式,PBL模式有诸多优点.它可以为学习者提供成功体验、替代经验、言语劝说及积极的情感体验,而这些正是自我效能的四种主要来源.但目前国内外相关实证研究还很少.通过实验研究,笔者发现PBL模式学习能够显著提高小学生的英语自我效能.此外,笔者还通过调查研究弄清了其途径,并以此为依据提出了对英语教学的启示.%Self-efficacy is people's subjective judgment on whether they can successfully complete a certain task, and it has significant correlation with academic achievement. As a learn-er-centered learning model, PBL model has many advantages. It can provide learners with successful experience, vicarious expe-rience, verbal persuasion and positive emotional experience which are the very four sources of self-efficacy. But there are few relevant empirical studies at home and abroad currently. Through experimental studies, the writer found that PBL model can signif-icantly improve the self-efficacy of primary school English. Be-sides, the writer also made clear its approaches through investi-gations and studies, and proposed the inspirations for English teaching based on this.

  16. Analysis of Status of Self-esteem of College Students in the Public and Pri-mary Medical Colleges%公办与民办医学院校大学生自尊现状分析

    Institute of Scientific and Technical Information of China (English)

    徐洪岩; 傅茂笋

    2016-01-01

    大学生的自尊与心理健康有着密不可分的联系,自尊已经成为大学生心理健康的关键指标。如今,民办与公办医学院校大学生自尊存在很大的差异性,导致这种差异存在的因素比较多。该文详细分析大学生自尊现状以及差异性存在的原因,认为应在全员育人理念的指导下,有针对性地进行分析研究,并针对存在的问题及时调整对策。%With the rapid development of society and ever increasing social rhythm, the employment pressure of college students is rising, and in terms of age, the college students have entered into the adult stage, but their minds are not very mature, facing the fierce social competition, they are difficult to truly resolve the confusion, thus leading to mental issues. The research shows that the self-esteem of college students is closely related to the mental health, and the self-esteem has been the key indicator of mental health of college students, now, there is a big difference in the self-esteem of college stu-dents between the public and primary medical colleges with many causes, and the teachers and education managers in the medical colleges must analyze the difference causes of self-esteem of college students in detail, and we should carry out the targeted analysis and research and adjust strategies in time for the existing issues under the concept of total education.

  17. Dosage of loading at elementary training technique of receptions of game volleyball of tall girls 13-14 years [Dozirovanie nagruzki pri nachal'nom obuchenii tekhnike priemov igry volejbol vysokoroslykh devochek 13-14 let

    Directory of Open Access Journals (Sweden)

    Selezneva O.V.

    2011-11-01

    Full Text Available Considered features of the elementary training technique basic the reception of playing volley-ball of tall girls. These intensities of implementation of exercises are resulted on physical and technical preparation. It is marked that the trainings loadings must be determined the followings indexes: intensity of work, volume (by duration of work, duration and character of intervals of rest between separate exercises. It is set that on the first stage of teaching the index of intensity of implementation of exercises makes 100-120 shots/minute. It is marked that on the second stage this index makes 120-140 shots/minute. On the third stage - 140-160 shots/minute. In this period of exercise on physical preparation must be executed with maximal intensity.

  18. 对少数民族双语教育幼小衔接的思考%A Discussion on the Linking Between Kindergarten and Pri mary School in Bilingual Education for Ethnic Minority Groups

    Institute of Scientific and Technical Information of China (English)

    帕丽达·阿哈斯; 王善安

    2012-01-01

      搞好少数民族双语教育的幼小衔接有利于多元文化的发展,帮助儿童语言及学业成绩的提高。双语教育幼小衔接的目标可以分为直接目标与间接目标。在实施双语教育幼小衔接时应注意双语教育目标的小步化,双语教育内容生活化,双语教育组织统整化,双语教育实施活动化。%  The linking between kindergarten and primary school in bilingual education for ethnic minority groups is important for the multicultural development of Xinjiang and for the improvement of ethnic children 's language and education.The target of linking program consists of direct goal and indirect goal .In the practice of the linking project, the target of bilingual education should be reachable , the content of the bilingual educa-tion should be connected with their daily life , and the structure of the bilingual education should be systemic , and the teaching mode of the bilingual education should be activity -oriented.

  19. Analysis on the Application of Stratified Teaching to Pri- mary School Mathematics Classroom Teaching%分层教学在小学数学课堂教学中运用的分析

    Institute of Scientific and Technical Information of China (English)

    倪晓燕

    2013-01-01

      分层教学如果恰当地运用到小学数学课堂上,能够满足学习水平存在差异学生的不同需求、促进数学教学水平的提高。本文分析了分层教学的意义以及在应用中应注意一些问题,以期为教学提供参考。%The application of stratified teaching to primary school mathematics classroom teaching can meet students' different needs in accordance with their different levels, so it can improve the teaching level of mathematics. This paper analyzes the signif-icance of stratified teaching and the issues that should be noticed in its application.

  20. Remedies to Fraud in Documentary Letters of Credit: A Comparative Perspective / Opravné Opatrenia Pri Podvodoch S Dokumentárnymi Akreditívami: Komparatívna Perspektíva

    Directory of Open Access Journals (Sweden)

    Alavi Hamed

    2016-06-01

    Full Text Available Article 4 of the Unified Customs and Practices of Documentary Letters of Credit establishes the notion of autonomy principle by separating credit from underlying contract between account party and beneficiary. Article 5 by recognizing the autonomy principle confirms that effectuate the payment under credit, banks only deal with documents and not with goods. As a result, while documentary letters of credit are meant to facilitate the process of international trade, their sole dependency on compliance of presented documents to bank by beneficiary to actualize the payment will increase the risk of fraud and forgery in the course of their operation. Interestingly, UCP (currently UCP600 takes a silent status regarding the problem of fraud in international LC operation and leaves the ground open for national laws to provide remedies to affected parties by fraudulent beneficiary. National Laws have different approaches to the problem of fraud in general and fraud in international LC operation in particular which makes the access of affected parties to possible remedies complicated and difficult. Current paper tries to find answer to the questions of (i what available remedies are provided to affected parties in international LC fraud by different legal systems? (ii And what are conditions for benefiting from such remedies under different legal systems? In achieving its objective, paper will be divided in two main parts to study remedies provided by intentional legal frameworks as well as the ones offered by national laws. Part one will study the position of UCP and UNCITRAL Convention on Independent Guarantees and Standby Letters of Credit (UNCITRAL Convention and remedies, which they provide to LC fraud in international trade. Part two in contrary will study available remedies to LC fraud and condition for access them under English and American legal system.

  1. Using didactic enclosures as teaching methods and social forms for active learning in chemical education: Nekateri didaktični sklopi učnih metod/socialnih oblik aktivnega učenja pri predmetu kemija:

    OpenAIRE

    Sikošek, Darinka

    2008-01-01

    Innovative teaching and learning can be defined as the effective use of various didactic materials combined with the methods and social forms of active learning. We carried out an evaluation of didactic enclosures as the initial stage in the methodology of our scientific strategy for researching the project "Didactic Redevelopment of Chemical Education in Slovenian Elementary Education". To obtain the results of our evaluation of didactic enclosures we concentrated on the innovative approach ...

  2. Plan Estratégico de Mercadeo PriCo Soluciones de Movilidad Posicionamiento y Ampliación del Mercado para el Servicio Administración y Logística de Parqueaderos en Cali.

    OpenAIRE

    2012-01-01

    El objetivo principal del Plan de Mercadeo está basado en definir estrategias y desarrollar programas que permitan posicionar su servicio administración y logística de parqueaderos en la ciudad de Cali, así como también ampliar su mercado atendido. Para el alcance del objetivo identificamos los factores que afectan la movilidad en la ciudad de Cali relacionados principalmente con el estacionamiento vehicular; así mismo segmentar el mercado permite el desarrollo de estrategias enfocadas en ...

  3. Segmentácia trhu pri použití marketingových dát ako súčasť marketingovej stratégie

    OpenAIRE

    Nagyová-Tkáčová, Monika

    2013-01-01

    The thesis deals mainly with market segmentation which is very important part of marketing strategy. The theoretical part consists of general knowledge of marketing, a Customer relationship management (CRM), market segmentation and database marketing. A case study consists of the implementation of the theoretical knowledge into the practical part. The aims of the thesis are: an introduction to the issue of market segmentation, an importance of market segmentation in creating marketing strateg...

  4. CFG桩清泥处理时机选择研究邹艳萍(1979.),女,工程师%Research on timing choice for silt-cleaning treatment of CFG pries

    Institute of Scientific and Technical Information of China (English)

    杨帅; 邹艳萍

    2011-01-01

    以某高铁路基工程为例,针对CFG桩清泥处理时机选择进行了研究,结合管内泵压混合料灌注流程,分别阐述了钻泥清理方法选择和湿法机械清理钻泥时机选择,并指出了清理钻泥注意事项,以指导今后CFG桩施工。%Taking the foundation project of some express railway as the example, the paper researches the timing choice for silt-cleaning treat- ment of CFG piles, illustrates the cleaning method of drilling silt and the drilling timing choice for the wet mechanic silt cleaning by combining with the grouting procedure for the pumped and injected with grouting mixture, and points out the precautions for the silt-cleaning, so as to direct the future CFG pile construction.

  5. Aplikacija teoretičnega modela Imogene M. King pri obravnavi starostnika s sladkorno boleznijo: Aplication of the Imogene M. King's nursing model in the treatment of elderly diabetes patients:

    OpenAIRE

    2009-01-01

    Introduction: The paper defines the problem of aging and presents the types ofdiabetes according to the American Diabetes Association, epidemiology of thedisease and the elderly diabetes patients in their social roles within the family and society. The basic principles of therapeutic communication with an elderly person are described. Imogene M. King's nursing model of goal attainment was used to ensure the holistic approach and treatment of the diseased elderly. Aim: The goal of the study wa...

  6. La crisis de la seguridad pública y los resultados electorales en el retorno del PRI a Nuevo León. Un análisis retrospectivo

    Directory of Open Access Journals (Sweden)

    Laura Nelly Medellín Mendoza

    2016-01-01

    Full Text Available En este artículo se revisa la problemática reciente de la seguridad en la agenda pública de Nuevo León. Se destaca el efecto en los resultados electorales para el partido gobernante, tanto en elecciones intermedias como en la sucesión por la gubernatura. En ese sentido, el periodo de gobierno de 2003 a 2009 tuvo que enfrentar los altos índices delictivos con promulgación de leyes y políticas públicas, para marcar una reestructuración institucional en esta área. Se encontró evidencia de que el partido gobernante perdió espacios en los comicios intermedios en un contexto de gran inseguridad, pero esto no repercutió en la sucesión por la gubernatura, aunque los índices de homicidios por narcotráfico y la percepción de inseguridad ciudadana fueron altos hasta el término de la administración.

  7. Uso de la inmunohistoquímica para la búsqueda de proteínas priónicas en tercer párpado de ovinos en Chile

    Directory of Open Access Journals (Sweden)

    G Farías

    2014-01-01

    Full Text Available En este trabajo se implementó y aplicó un método para detectar el scrapie, utilizando la técnica de inmunohistoquímica (IHQ en tejido linfoide de tercer párpado ovino. Este método permitiría realizar el diagnóstico en animales vivos, incluso en fases preclínicas de esta enfermedad en la masa ovina nacional. Se obtuvieron y procesaron 80 muestras de tercer párpado de ovinos nacionales mayores de 2 años, 42 con sintomatología nerviosa compatible con scrapie y 38 que fueron obtenidos de mataderos. Otras 13 muestras ciegas de validación de tercer párpado y sus respectivos óbex, además de 16 muestras de óbex utilizadas como controles, fueron aportadas por un Centro de Referencia (CR. Las muestras fueron evaluadas en cortes teñidos con Hematoxilina-Eosina y clasificadas como aptas cuando presentaban al menos 4 folículos linfoides en el tejido obtenido. Sobre esta base, 74 de las 93 muestras totales de tercer párpado fueron consideradas como aptas. Los resultados de IHQ mostraron que todas las muestras nacionales fueron negativas a scrapie y sólo 7 de las proporcionadas por el CR fueron positivas. Además, en todos los controles positivos de óbex se observó inmunomarcaje de PrP Sc, lo cual no se evidenció en los controles negativos. Los resultados descritos anteriormente permiten demostrar la aplicabilidad y capacidad para utilizar esta herramienta diagnóstica en la masa ovina, lo cual permitiría establecer la condición del país respecto al scrapie y obtener los beneficios de esta situación sanitaria.

  8. Role of tectonics, volcanism and climate during sedimentation of the Autunian series in the Inner Sudeten basin. Role tektoniky, vulkanizmu a klimatu pri sedimentaci olivetinskych vrstev (Autun) ve Vnitrosudetske panvi

    Energy Technology Data Exchange (ETDEWEB)

    Blecha, M. (Univerzita Karlova, Prague (Czech Republic). Prirodovedecka Fakulta)

    1993-01-01

    Discusses paleoenvironment of the Autunian series in the Inner Sudeten basin with coal-bearing strata. The following aspects are evaluated: position of the Autunian series (to 200 m thick) in the basin, types of sedimentary rocks, sequences of sedimentation and their interpretation, alluvial sediments, river depositions, saline lakes and their deposits, manifestations of tectonic activity, deposit tectonics, effects of tectonics on strata inclination, secondary lake deposits, volcanic activity and types of volcanic rocks. The following stages of deposit accumulation are analyzed: alluvial sedimentation, volcanism, erosion, deposition of volcanic breccia in low zones, repeated deposition, effects of climate changes. 11 refs.

  9. Complex Coherent Interference Suppression Method Based on Random Variable PRI%基于随机变 T 的抗复合相干干扰方法的目标检测性能

    Institute of Scientific and Technical Information of China (English)

    朱振波; 汤子跃; 王亚洲; 马少博

    2015-01-01

    Coherent radar is jammed through modulating and repeating the radar signals based on detecting and recognizing radar signal parameter. To confront the enemy reconnaissance effec-tively, a method of two pulses MTI cascade sliding window (non-coherent) detection is put for-ward. Compared to full coherent work, the radar performance of this method is declined, but it can work under the background of composite coherent jamming. Theoretical analysis and simula-tion results demonstrate the effectiveness and universality of this method.%现代干扰机能通过侦察识别雷达发射信号参数,并调制转发以实现对相参雷达的干扰。为有效对抗敌方侦察,针对上述方式,提出了随机变 T+2脉冲 MTI 再级联滑窗(非相参)检测的抗干扰方法。该方法较全相参工作时性能略有下降,但提升了雷达在复合相关干扰背景下的工作性能,仿真结果证明了该方法的有效性。

  10. Femoral Neck Fracture Treated with Percutaneous Pryed Reduction and External Fixation Apparatus:105 Cases Report%撬拨复位外固定治疗股骨颈头颈型骨折(附105例报告)

    Institute of Scientific and Technical Information of China (English)

    金军; 徐海涛; 杨顺; 苏纪权; 刘志升

    2004-01-01

    目的:探讨钢针撬拨复位在治疗股骨颈骨折中的应用价值.方法:对105例股骨颈头颈型骨折施行钢针撬拨复位结合三针锁针加压器治疗,术后随访观察骨折愈合优良率及后期股骨头坏死率.结果:105例病人均获得随访.随访时间2.5~6年,平均3.5年,其中:功能接近正常者共91例,占86.6%.骨折愈合94例,占89.5%.其中并发股骨头坏死6例.骨折不愈合10例,占9.5%.死亡1例,占0.9%.无1例院内感染.结论:钢针撬拨复位可使骨折断端紧密对合,达到解剖复位,只要掌握手术适应证,遵循手术操作原则,术后针道常规处置,半介入式外固定器的并发症一般是可以避免的,因此有着良好的应用前景.

  11. An Accurate Estimation for PRI Rate-of-Change with the Coherent Pulse Train%相参脉冲序列PRI变化率的高精度估计方法

    Institute of Scientific and Technical Information of China (English)

    周龙健; 罗景青

    2014-01-01

    在单站无源定位与跟踪系统中,PRI变化率信息反映了运动目标的径向加速度信息.提出了一种新的PRI变化率估计的算法,利用脉冲载频之间的相参积累,使信噪比得到提高,通过群延迟,使PRI的变化量得到积累,推导出一种快速而精确测量PRI变化率的算法,数值仿真证明使用该算法可以精确快速地估计信号中的PRI变化率信息.

  12. A Radar Signal Main Sorting Algorithm Based on PRI Entropy and Transfer Matrix%基于PRI熵和转移矩阵的雷达信号主分选算法

    Institute of Scientific and Technical Information of China (English)

    李星雨; 杨承志; 张荣

    2013-01-01

    雷达信号实时分选是电子侦察的关键技术之一,针对传统主分选算法存在的缺陷,提出一种基于PRI熵和转移矩阵的主分选算法.该方法采用先整体后局部的思想,首先通过PRI熵信息找到复杂度低的区域,然后统计转移矩阵完成PRI估计,最后进行前后序列搜索.仿真实验验证了该方法的有效性.

  13. PRI信令在电力系统通信中出现2M板闭锁的问题%Problem of the PRI Signaling in Power Grid Communication Creating Barring of 2M Board

    Institute of Scientific and Technical Information of China (English)

    万清

    2006-01-01

    电力通信程控交换机的技术发展很快,以ISDN PRI信令组网方式在电力通信网中得到了广泛的应用,ISDN PRI信令组网优势越来越明显.由于PRI信令在实际过程中会出现2 M板闭锁(退出服务)的问题,对此介绍了所产生的几种主要原因和故障排除的方法和处理流程.

  14. Non-Canonical Processing of Arabidopsis pri-miR319a/b/c Generates Additional microRNAs to Target One RAP2.12 mRNA Isoform.

    Science.gov (United States)

    Sobkowiak, Lukasz; Karlowski, Wojciech; Jarmolowski, Artur; Szweykowska-Kulinska, Zofia

    2012-01-01

    Arabidopsis miR319a/b/c primary transcripts are unusual due to the presence of a long stem and loop structure containing functional miR319a/b/c molecules. In our experiments carried out using high throughput sequencing (HTS), we have shown that additional microRNAs (miRNAs), miR319a.2/b.2/c.2 are generated from the upper part of the same hairpin structure. We have also found cognate miRNAa.2*/b.2*/c.2* to be present in the HTS results with a considerably lower number of reads. Northern hybridization revealed that miR319b.2 is mainly expressed in 35-day-old plant rosette leaves, as well as in stem and inflorescences of 42- and 53-day-old plants. Moreover, it carries multiple signatures of a functional miRNA, including as follows: (i) its biogenesis is HYL1-dependent; (ii) it is incorporated in a substantial amount into RISC complexes containing AGO1, AGO2, or AGO4 protein; (iii) 24 nt-long species of miR319b.2 have been found in inflorescences to be more abundant than 21 nt miR319b.2 species; (iv) it is present in various ratios to miR319b during plant development, which suggests the existence of a regulatory mechanism responsible for its biogenesis/processing; (v) there is an observed cross-species conservation of the miR319a/b/c stem nucleotide sequence extending beyond mature miRNA region; and (vi) all evidence suggests that intron-containing RAP2.12 mRNA isoform is the target for miR319b.2. All these features prompt us to claim miR319b.2 as a functional miRNA molecule.

  15. SNP-SNP interactions of three new pri-miRNAs with the target gene PGC and multidimensional analysis of H. pylori in the gastric cancer/atrophic gastritis risk in a Chinese population.

    Science.gov (United States)

    Xu, Qian; Wu, Ye-Feng; Li, Ying; He, Cai-Yun; Sun, Li-Ping; Liu, Jing-Wei; Yuan, Yuan

    2016-04-26

    Gastric cancer (GC) is a multistep complex disease involving multiple genes, and gene-gene interactions have a greater effect than a single gene in determining cancer susceptibility. This study aimed to explore the interaction of the let-7e rs8111742, miR-365b rs121224, and miR-4795 rs1002765 single nucleotide polymorphisms (SNPs) with SNPs of the predicted target gene PGC and Helicobacter pylori status in GC and atrophic gastritis (AG) risk. Three miRNA SNPs and seven PGC SNPs were detected in 2448 cases using the Sequenom MassArray platform. Two pairwise combinations of miRNA and PGC SNPs were associated with increased AG risk (let-7e rs8111742 - PGC rs6458238 and miR-4795 rs1002765 - PGC rs9471643). Singly, miR-365b rs121224 and PGC rs6912200 had no effect individually but in combination they demonstrated an epistatic interaction associated with AG risk. Similarly, let-7e rs8111742 and miR-4795 rs1002765 SNPs interacted with H. pylori infection to increase GC risk (rs8111742: Pinteraction = 0.024; rs1002765: Pinteraction = 0.031, respectively). A three-dimensional interaction analysis found miR-4795 rs1002765, PGC rs9471643, and H. pylori infection positively interacted to increase AG risk (Pinteraction = 0.027). Also, let-7e rs8111742, PGC rs6458238, and H. pylori infection positively interacted to increase GC risk (Pinteraction = 0.036). Furthermore, both of these three-dimensional interactions had a dosage-effect correspondence (Ptrend < 0.001) and were verified by MDR. In conclusion, the miRNAs SNPs (let-7e rs8111742 and miR-4795 rs1002765) might have more superior efficiency when combined with PGC SNPs and/or H. pylori for GC or AG risk than a single SNP on its own.

  16. 一种PRI变换并行处理算法及在高速DSP上的实现%Parallel Processing Algorithm for PRI Transform and Realization Based on High Speed DSP Platform

    Institute of Scientific and Technical Information of China (English)

    鲍庆龙; 杨剑; 陈曾平

    2009-01-01

    脉冲重复间隔是雷达信号分选的关键参数.改进的PRI变换法克服了直方图统计法的子谐波问题,对脉冲重复间隔具有很高的估计精度,且具有一定的抗抖动能力.由于需要进行大量的指数运算和复数运算,故PRI变换法运算速度较慢,难以满足实时性要求.本文分析了改进的PRI变换法,提出了一种用于该算法并行处理的任务分配原则,并在TS201S平台上实现了改进的PRI变换算法.实验结果表明,基于该任务分配原则的PRI变换法并行处理大大提高了运算效率,能满足实时性要求.

  17. The optimization and application of PRI mode connecting with PBX in Ningxia Electric Power Administrative Exchange Network%PRI方式接入PBX在宁夏电力行政交换网中的优化和应用

    Institute of Scientific and Technical Information of China (English)

    施科峰

    2009-01-01

    通过实例介绍了在宁夏电力行政交换网中,运用基群速率综合业务服务网(pRI)信令方式接入某些电厂或变电站的专用分组语音交换机(PBX)设备的两种方式.通过分析对比得到优选的接入方式,阐述了其实际推广使用的价值.

  18. 基于PRI接口的集中录音方式的应用%The Application of Centralized Recording Method in Power Communication System Based on PRI Interface

    Institute of Scientific and Technical Information of China (English)

    施维刚

    2010-01-01

    基于ISDN的PRI接口在电力交换专网得到广泛应用,通过PRI接口实现集中录音功能既可以调高录音质量还可以提高录音系统的可靠性.文章通过MDR2000N录音记录仪与Coral交换机之间实现PRI接口集中录音的实例,说明了集中录音的实现原理和过程,并对实施过程中出现的主要问题进行了分析和说明,对基于PRI接口的集中录音方式在电力通信中的应用具有一定的借鉴作用.

  19. Type 1 Diabetes in the Young: Organization of two National Centers in Israel and Slovenia / Sladkorna Bolezen Tipa 1 Pri Otrocih in Mladostnikih: Organizacija Dela V Dveh Nacionalnih Centrih V Izraelu in Sloveniji

    Directory of Open Access Journals (Sweden)

    Bratina Nataša

    2015-03-01

    Full Text Available Sladkorna bolezen tipa 1 je kronično avtoimuno obolenje, ki najpogosteje prizadene mlade ljudi. Incidenca bolezni zadnjih 50 let narašča po vsem svetu, v povprečju je letni porast incidence ocenjen na 4 %, ob tem pa starost bolnikov ob času diagnoze pada. Raziskave kažejo, da je dobra presnovna urejenost bolnikov s sladkorno boleznijo zelo pomembna, saj je s tem mogoče odložiti pozne zaplete bolezni ter izboljšati kvaliteto življenja bolnikov in njihovih družin. Neprekinjena edukacija bolnikov skupaj z možnostmi moderne tehnologije lahko prispeva k izboljšanju njihove presnovne urejenosti. Presnovno urejenost spremljamo z določanjem glikoziliranega hemoglobina (HbA1c ter s številom težkih hipoglikemij in ketoacidoz na populacijo pacientov v določenem časovnem obdobju. V prispevku sta opisana dva nacionalna referenčna centra za obravnavo otrok, mladostnikov in mladih odraslih s sladkorno boleznijo v Izraelu in Sloveniji.

  20. Antiproliferative Activity and in Vivo Toxicity of Double-Point Modified Analogs of 1,25-Dihydroxyergocalciferol.

    Science.gov (United States)

    Trynda, Justyna; Turlej, Eliza; Milczarek, Magdalena; Pietraszek, Anita; Chodyński, Michał; Kutner, Andrzej; Wietrzyk, Joanna

    2015-10-20

    Analogs of 1,25-dihydroxyergocalciferol, modified in the side-chain and in the A-ring, were tested for their antiproliferative activity against a series of human cancer cell lines in vitro and in vivo toxicity. The proliferation inhibition caused by the analogs was higher than that of the parent compounds, while the toxicity, measured as the serum calcium level, was lower. All analogs were able to induce, in HL-60 and MV4-11 leukemic cells, G₀/G₁ cell cycle arrest and differentiation expressed as morphological signs typical for monocytes. The analogs also induced the expression of CD11b and/or CD14 cell-differentiation markers. The most potent analogs, PRI-5105, PRI-5106, PRI-5201 and PRI-5202, were also able to induce vitamin D receptor (VDR) protein expression, mainly in the cytoplasmic fraction of HL-60 or MV4-11 cells. The most active analogs were the 19-nor ones with an extended and rigidified side-chain (PRI-5201 and PRI-5202), as in the former analogs PRI-1906 and PRI-1907. Epimerization at C-24 (PRI-5101) or introduction of an additional hydroxyl at C-23 (PRI-5104) reduced the toxicity of the analog with retained antiproliferative activity.

  1. Antiproliferative Activity and in Vivo Toxicity of Double-Point Modified Analogs of 1,25-Dihydroxyergocalciferol

    Directory of Open Access Journals (Sweden)

    Justyna Trynda

    2015-10-01

    Full Text Available Analogs of 1,25-dihydroxyergocalciferol, modified in the side-chain and in the A-ring, were tested for their antiproliferative activity against a series of human cancer cell lines in vitro and in vivo toxicity. The proliferation inhibition caused by the analogs was higher than that of the parent compounds, while the toxicity, measured as the serum calcium level, was lower. All analogs were able to induce, in HL-60 and MV4-11 leukemic cells, G0/G1 cell cycle arrest and differentiation expressed as morphological signs typical for monocytes. The analogs also induced the expression of CD11b and/or CD14 cell-differentiation markers. The most potent analogs, PRI-5105, PRI-5106, PRI-5201 and PRI-5202, were also able to induce vitamin D receptor (VDR protein expression, mainly in the cytoplasmic fraction of HL-60 or MV4-11 cells. The most active analogs were the 19-nor ones with an extended and rigidified side-chain (PRI-5201 and PRI-5202, as in the former analogs PRI-1906 and PRI-1907. Epimerization at C-24 (PRI-5101 or introduction of an additional hydroxyl at C-23 (PRI-5104 reduced the toxicity of the analog with retained antiproliferative activity.

  2. A propene-substituted cyclotriphosphazene : Synthesis, characterization and polymerization

    NARCIS (Netherlands)

    Bosscher, G; Meetsma, A; vandeGrampel, JC

    1997-01-01

    The elimination reaction of the sulfonium-substituted chlorocyclotriphosphazene N3P3Cl4Pri(CMe2OSO2Me) with 1,8-diazabicyclo[5.4.0]undec-7-ene afforded a new olefin-substituted chlorocyclotriphosphazene, N3P3Cl4Pri(CMe=CH2), and an ansa derivative with formula N3P3Cl3Pri(CMe2CH=CMeO). The crystal st

  3. Arterijska hipertenzija in DASH dieta

    OpenAIRE

    Vidović, Duška

    2017-01-01

    Uvod: Arterijska hipertenzija je pri nas in v razvitem svetu poglavitni vzrok obolevnosti in umrljivosti, zato predstavlja velik javno-zdravstveni problem. Namen: Namen diplomskega dela je na teoretični ravni predstaviti arterijsko hipertenzijo, dejavnike tveganja za nastanek arterijske hipertenzije in zdravljenje s poudarkom na predstavitvi DASH diete, ki je oblika pravilnega prehranjevanja pri obolenju oseb z visokim krvnim tlakom. Diplomsko delo želi predstaviti vlogo medicinske sestre pri...

  4. Mitigating Corruption in Government Security Forces: The Role of Institutions, Incentives, and Personnel Management in Mexico

    Science.gov (United States)

    2011-01-01

    Preventive Police PJF Policía Judicial Federal, or Federal Judicial Police PRI Partido Revolucionario Institucional , or Institutional Revolutionary...Partido Revolucionario Institucional , or PRI) emerged as the dominating political force in Mexico in the 20th century. The PRI regime was... tradicional /default.aspx Jacob, Brian A., and Steven D. Levitt, “Rotten Apples: An Investigation of the Prevalence and Predictors of Teacher Cheating

  5. Remedial sanation arthroscopy in gouty arthropathy of the knee joint

    Directory of Open Access Journals (Sweden)

    V V Lyalina

    2007-01-01

    Zaklyuchenie. Predlozhennaya avtorami metodika sanatsionnoy artroskopii znachitel'no rasshiryaet spektr lechebnykh vozmozhnostey i pozvolyaet optimizirovat' lechebnuyu taktiku pri khronicheskom podagricheskom artrite kolennogo sustava.

  6. GOVORNE NAPAKE PREDŠOLSKIH OTRKO IN SODELOVANJE LOGOPEDA IN VZGOJITELJA V VRTCU

    OpenAIRE

    Praznik, Stanislava

    2013-01-01

    Diplomsko delo opisuje govorne napake pri predšolskih otrocih in sodelovanje vzgojiteljice in logopeda v vrtcu in sodelovanje vzgojiteljice in logopeda, ki ne dela v vrtcu. V teoretičnem delu je najprej opisan razvoj govora in faze govornega razvoja, govorne napake, delo logopeda in teorije o učenju govora, pomoč in vaje pri učenju govora in vloga odraslega pri učenju govora. Zavedati se moramo, da je govor prvo komunikacijsko sredstvo, ki ga uporabljamo. Pomoč vzgojiteljice pri razvoju go...

  7. Program Energy of the CNRS. Topic 10 combustion and capture of CO{sub 2}. PRI 10.1. Capture by adsorption of the CO{sub 2} in thermal power plants gas and their injection in petroleum wells. Final report period 2002-2004; Programme energie du CNRS. Theme 10 combustion et capture du CO2. PRI 10.1. Capture par adsorption du CO2 dans des gaz de centrales thermiques et leur injection en puits de petrole. Rapport final periode 2002-2004

    Energy Technology Data Exchange (ETDEWEB)

    Tondeur, D

    2005-07-01

    In the framework of the global warming resulting of the greenhouse gases emission increase, the carbon dioxide capture and storage in deep underground cavities of old petroleum and gas deposits, are studied. This report presents the researches realized by the CNRS (France) in the domain: technology and knowledge assessment concerning the carbon dioxide capture and storage, active coals for the CO{sub 2} capture, methodology of thermo-economical optimization of the combined cycle, global simulation of an IGCC (Integrated gasification combined cycle) with CO{sub 2} capture and integration in the process scheme, petroleum recovery-aided by CO{sub 2} injection, storage in geological deposits. (A.L.B.)

  8. GenBank blastx search result: AK060171 [KOME

    Lifescience Database Archive (English)

    Full Text Available 1 (splicing factor 2, alternate splicing factor), mRNA (cDNA clone MGC:5228 IMAGE:2900101), complete cds.|PRI PRI 2e-14 +1 ... ...AK060171 006-311-F07 BC010264.1 Homo sapiens splicing factor, arginine/serine-rich

  9. GenBank blastx search result: AK061903 [KOME

    Lifescience Database Archive (English)

    Full Text Available 1 (splicing factor 2, alternate splicing factor), mRNA (cDNA clone MGC:45185 IMAGE:5166554), complete cds.|PRI PRI 6e-47 +1 ... ...AK061903 001-041-H07 BC033785.1 Homo sapiens splicing factor, arginine/serine-rich

  10. GenBank blastx search result: AK060171 [KOME

    Lifescience Database Archive (English)

    Full Text Available 1 (splicing factor 2, alternate splicing factor), mRNA (cDNA clone MGC:45185 IMAGE:5166554), complete cds.|PRI PRI 2e-14 +1 ... ...AK060171 006-311-F07 BC033785.1 Homo sapiens splicing factor, arginine/serine-rich

  11. GenBank blastx search result: AK106998 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK106998 002-120-B12 AB179082.1 Macaca fascicularis testis cDNA clone: QtsA-12630, similar to human oculocer...ebrorenal syndrome of Lowe (OCRL), transcriptvariant a, mRNA, RefSeq: NM_000276.3.|PRI PRI 1e-29 +3 ...

  12. GenBank blastx search result: AK061708 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061708 001-037-F11 AF023268.1 Homo sapiens clk2 kinase (CLK2), propin1, cote1, glucocerebro...sidase (GBA), and metaxin genes, complete cds; metaxin pseudogene and glucocerebrosidase pseudogene; and thrombospondin3 (THBS3) gene, partial cds.|PRI PRI 1e-41 +3 ...

  13. GenBank blastx search result: AK104229 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK104229 006-307-D03 AF023268.1 Homo sapiens clk2 kinase (CLK2), propin1, cote1, glucocerebro...sidase (GBA), and metaxin genes, complete cds; metaxin pseudogene and glucocerebrosidase pseudogene; and thrombospondin3 (THBS3) gene, partial cds.|PRI PRI 3e-41 +3 ...

  14. GenBank blastx search result: AK059494 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059494 001-028-H05 AB179082.1 Macaca fascicularis testis cDNA clone: QtsA-12630, similar to human oculocer...ebrorenal syndrome of Lowe (OCRL), transcriptvariant a, mRNA, RefSeq: NM_000276.3.|PRI PRI 7e-40 +2 ...

  15. GenBank blastx search result: AK058642 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058642 001-018-E05 AF023268.1 Homo sapiens clk2 kinase (CLK2), propin1, cote1, glucocerebro...sidase (GBA), and metaxin genes, complete cds; metaxin pseudogene and glucocerebrosidase pseudogene; and thrombospondin3 (THBS3) gene, partial cds.|PRI PRI 2e-18 +3 ...

  16. GenBank blastx search result: AK060447 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060447 001-011-F08 AY163769.1 Homo sapiens excision repair cross-complementing ro...dent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3) gene, complete cds.|PRI PRI 1e-155 +1 ...

  17. GenBank blastx search result: AK060447 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060447 001-011-F08 BC008820.2 Homo sapiens excision repair cross-complementing ro...dent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing), mRNA (cDNA clone MGC:10371 IMAGE:3958781), complete cds.|PRI PRI 1e-155 +1 ...

  18. GenBank blastx search result: AK104850 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK104850 001-043-A07 AY204752.1 Homo sapiens excision repair cross-complementing ro...dent repair deficiency, complementation group 6 (ERCC6) gene, complete cds.|PRI PRI 1e-12 +3 ...

  19. GenBank blastx search result: AK104850 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK104850 001-043-A07 AB209504.1 Homo sapiens mRNA for excision repair cross-complement...ing rodent repair deficiency, complementation group 6 variant protein.|PRI PRI 1e-12 +3 ...

  20. GenBank blastx search result: AK060447 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060447 001-011-F08 AK222465.1 Homo sapiens mRNA for excision repair cross-complement...ing rodent repair deficiency, complementation group 3 variant, clone: adKA02463.|PRI PRI 1e-153 +1 ...

  1. Comparative effects of partial root-zone irrigation and deficit irrigation on phosphorus uptake in tomato plants

    DEFF Research Database (Denmark)

    Wang, Yaosheng; Liu, Fulai; Jensen, Christian Richardt

    2012-01-01

    The comparative effects of partial root-zone irrigation (PRI) and deficit irrigation (DI) on phosphorus (P) uptake in tomato (Lycopersicon esculentum Mill.) plants were investigated in a split-root pot experiment. The results showed that PRI treatment improved water-use efficiency (WUE) compared...

  2. GenBank blastx search result: AK107538 [KOME

    Lifescience Database Archive (English)

    Full Text Available some 10 Contains a novel gene, the HPS1 gene for Hermansky-Pudlak syndrome 1, the 3' end of the HPSE2 gene for heparanase 2 and a CpG island, complete sequence.|PRI PRI 5e-21 +2 ...

  3. GenBank blastx search result: AK063915 [KOME

    Lifescience Database Archive (English)

    Full Text Available some 10 Contains a novel gene, the HPS1 gene for Hermansky-Pudlak syndrome 1, the 3' end of the HPSE2 gene for heparanase 2 and a CpG island, complete sequence.|PRI PRI 1e-139 +3 ...

  4. GenBank blastx search result: AK060767 [KOME

    Lifescience Database Archive (English)

    Full Text Available some 10 Contains a novel gene, the HPS1 gene for Hermansky-Pudlak syndrome 1, the 3' end of the HPSE2 gene for heparanase 2 and a CpG island, complete sequence.|PRI PRI 3e-25 +2 ...

  5. GenBank blastx search result: AK058594 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058594 001-017-H01 AB169480.1 Macaca fascicularis testis cDNA, clone: QtsA-21542, similar to human diaphor...ase (NADH) (cytochrome b-5 reductase) (DIA1),transcript variant M, mRNA, RefSeq: NM_000398.3.|PRI PRI 7e-53 +3 ...

  6. GenBank blastx search result: AK103964 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK103964 001-015-F12 AB169480.1 Macaca fascicularis testis cDNA, clone: QtsA-21542, similar to human diaphor...ase (NADH) (cytochrome b-5 reductase) (DIA1),transcript variant M, mRNA, RefSeq: NM_000398.3.|PRI PRI 7e-53 +3 ...

  7. GenBank blastx search result: AK106074 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK106074 001-206-H10 BC021102.1 Homo sapiens likely ortholog of mouse immediate early response, erythropoiet...in 4, mRNA (cDNA clone MGC:31837 IMAGE:5020890), complete cds.|PRI PRI 4e-52 +2 ...

  8. GenBank blastx search result: AK061903 [KOME

    Lifescience Database Archive (English)

    Full Text Available ernate splicing factor), mRNA (cDNA clone MGC:5228 IMAGE:2900101), complete cds.|PRI PRI 6e-47 +1 ... ...AK061903 001-041-H07 BC010264.1 Homo sapiens splicing factor, arginine/serine-rich 1 (splicing factor 2, alt

  9. GenBank blastx search result: AK119168 [KOME

    Lifescience Database Archive (English)

    Full Text Available ocyte-activating), mRNA (cDNA clone MGC:20063 IMAGE:3638796), complete cds.|PRI PRI 3e-36 +3 ... ...AK119168 001-036-F11 BC014051.2 Homo sapiens small inducible cytokine subfamily E, member 1 (endothelial mon

  10. GenBank blastx search result: AK108051 [KOME

    Lifescience Database Archive (English)

    Full Text Available ocyte-activating), mRNA (cDNA clone MGC:20063 IMAGE:3638796), complete cds.|PRI PRI 6e-33 +1 ... ...AK108051 002-137-H02 BC014051.2 Homo sapiens small inducible cytokine subfamily E, member 1 (endothelial mon

  11. GenBank blastx search result: AK059433 [KOME

    Lifescience Database Archive (English)

    Full Text Available ocyte-activating), mRNA (cDNA clone MGC:20063 IMAGE:3638796), complete cds.|PRI PRI 3e-15 +1 ... ...AK059433 001-027-F07 BC014051.2 Homo sapiens small inducible cytokine subfamily E, member 1 (endothelial mon

  12. GenBank blastx search result: AK059413 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059413 001-027-D02 AB063318.1 Homo sapiens MoDP-2, MoDP-3 mRNA for acute morphine... dependence related protein 2, acute morphine dependence related peptide 3, complete cds.|PRI PRI 9e-12 +1 ...

  13. GenBank blastx search result: AK062146 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK062146 001-045-G12 AB063318.1 Homo sapiens MoDP-2, MoDP-3 mRNA for acute morphine... dependence related protein 2, acute morphine dependence related peptide 3, complete cds.|PRI PRI 2e-80 +1 ...

  14. GenBank blastx search result: AK059663 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059663 001-031-E04 AB063318.1 Homo sapiens MoDP-2, MoDP-3 mRNA for acute morphine... dependence related protein 2, acute morphine dependence related peptide 3, complete cds.|PRI PRI 7e-29 +3 ...

  15. GenBank blastx search result: AK060220 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060220 001-002-F01 BC065912.1 Homo sapiens v-abl Abelson murine leukemia viral on...cogene homolog 2 (arg, Abelson-related gene), mRNA (cDNA clone MGC:70756 IMAGE:5173213), complete cds.|PRI PRI 1e-37 +3 ...

  16. GenBank blastx search result: AK061938 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061938 001-042-D07 BC065912.1 Homo sapiens v-abl Abelson murine leukemia viral on...cogene homolog 2 (arg, Abelson-related gene), mRNA (cDNA clone MGC:70756 IMAGE:5173213), complete cds.|PRI PRI 6e-26 +2 ...

  17. GenBank blastx search result: AK111983 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK111983 001-012-H11 BC065912.1 Homo sapiens v-abl Abelson murine leukemia viral on...cogene homolog 2 (arg, Abelson-related gene), mRNA (cDNA clone MGC:70756 IMAGE:5173213), complete cds.|PRI PRI 3e-34 +3 ...

  18. GenBank blastx search result: AK058580 [KOME

    Lifescience Database Archive (English)

    Full Text Available highly similar to DNA-DIRECTED RNA POLYMERASE I 135 KD POLYPEPTIDE (EC 2.7.7.6).|PRI PRI 1e-74 +1 ... ...AK058580 001-017-F06 AK022533.1 Homo sapiens cDNA FLJ12471 fis, clone NT2RM1000894,

  19. GenBank blastx search result: AK110106 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK110106 002-161-A03 AB179164.1 Macaca fascicularis testis cDNA clone: QtsA-14001, similar to human huntingt...in interacting protein 1 (HIP1), mRNA, RefSeq: NM_005338.4.|PRI PRI 2e-51 +1 ...

  20. GenBank blastx search result: AK105069 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK105069 001-045-C01 AB169286.1 Macaca fascicularis testis cDNA, clone: QtsA-18648, similar to human mortali...ty factor 4 like 2 (MORF4L2), mRNA, RefSeq: NM_012286.1.|PRI PRI 3e-20 +3 ...

  1. Internacia Esperanto--Movado

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    BelgioEn la belga magazino "Knack"(11 okt.1995) aperis 5-paa artikolo de L. Willems pri lamultlingveco de la mondo kaj pri Esperanto.BulgarioLa 14-a sesio de Internacia Somera E-Uni-versitato okazos en Veliko Tarnovo (Bulgario) de

  2. Uvod v tematski sklop

    Directory of Open Access Journals (Sweden)

    Florence Gacoin-Marks

    2009-12-01

    Slovenski raziskovalni prostor seveda premore veliko več zanimivih raziskav v zvezi z Afriko, zato lahko samo obžalujemo, da nekateri raziskovalci pri tej publikaciji niso mogli sodelovati in da zato njihova stroka tukaj ni zastopana. Ob tej priložnosti se iskreno zahvaljujem vsem, ki so sodelovali pri projektu s kakovostnimi in raznovrstnimi razpravami.

  3. GenBank blastn search result: AK059159 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059159 001-023-D03 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 3e-19 Plus Plus ...

  4. GenBank blastx search result: AK058227 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058227 001-010-H11 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 1e-16 +2 ...

  5. GenBank blastn search result: AK059019 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059019 001-021-B02 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 5e-47 Plus Minus ...

  6. GenBank blastn search result: AK058741 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058741 001-019-G08 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 2e-55 Plus Minus ...

  7. GenBank blastn search result: AK119240 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK119240 001-120-A04 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 6e-50 Plus Minus ...

  8. GenBank blastx search result: AK058913 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058913 001-009-A01 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 1e-27 +1 ...

  9. GenBank blastn search result: AK059098 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059098 001-022-C07 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 2e-43 Plus Minus ...

  10. GenBank blastn search result: AK058686 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058686 001-019-A10 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 6e-46 Plus Minus ...

  11. GenBank blastn search result: AK105816 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK105816 001-203-C11 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 1e-47 Plus Minus ...

  12. GenBank blastx search result: AK059228 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059228 001-024-D11 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 8e-27 +3 ...

  13. GenBank blastx search result: AK058540 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058540 001-017-B06 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 5e-27 +1 ...

  14. GenBank blastn search result: AK058436 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058436 001-015-F07 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 2e-51 Plus Minus ...

  15. GenBank blastx search result: AK061605 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061605 001-030-C05 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 7e-27 +1 ...

  16. GenBank blastn search result: AK058913 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058913 001-009-A01 U91328.1 Human hereditary haemochromatosis region, histone 2A-like protein gene, heredi...tary haemochromatosis (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds.|PRI PRI 3e-12 Plus Minus ...

  17. GenBank blastx search result: AK105716 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK105716 001-201-F04 CR457266.1 Homo sapiens full open reading frame cDNA clone RZP...Do834C046D for gene C20orf31, chromosome 20 open reading frame 31; complete cds, incl. stopcodon.|PRI PRI 1e-90 +1 ...

  18. GenBank blastx search result: AK059388 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK059388 001-026-H03 CR457345.1 Homo sapiens full open reading frame cDNA clone RZP...Do834B0510D for gene C14orf137, chromosome 14 open reading frame 137; complete cds, incl. stopcodon.|PRI PRI 1e-28 +3 ...

  19. GenBank blastx search result: AK060852 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060852 001-034-E10 CR457266.1 Homo sapiens full open reading frame cDNA clone RZP...Do834C046D for gene C20orf31, chromosome 20 open reading frame 31; complete cds, incl. stopcodon.|PRI PRI 3e-33 +3 ...

  20. GenBank blastx search result: AK107489 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107489 002-129-C12 CR457345.1 Homo sapiens full open reading frame cDNA clone RZP...Do834B0510D for gene C14orf137, chromosome 14 open reading frame 137; complete cds, incl. stopcodon.|PRI PRI 1e-14 +1 ...

  1. GenBank blastx search result: AK059494 [KOME

    Lifescience Database Archive (English)

    Full Text Available for 60kDa splicing factor 3a subunit 3, the FHL3 gene for four and a half LIM domains 3 and the gene for comparative gene identifica...tion transcript 94 (CGI-94), complete sequence.|PRI PRI 2e-37 +2 ...

  2. GenBank blastx search result: AK058710 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058710 001-019-D05 AK027699.1 Homo sapiens cDNA FLJ14793 fis, clone NT2RP4001174,... weakly similar to NON-GREEN PLASTID TRIOSE PHOSPHATE TRANSLOCATOR PRECURSOR.|PRI PRI 4e-14 +3 ...

  3. GenBank blastx search result: AK060577 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060577 001-023-E05 AK027699.1 Homo sapiens cDNA FLJ14793 fis, clone NT2RP4001174,... weakly similar to NON-GREEN PLASTID TRIOSE PHOSPHATE TRANSLOCATOR PRECURSOR.|PRI PRI 1e-18 +2 ...

  4. GenBank blastx search result: AK061991 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061991 001-043-C01 AK027699.1 Homo sapiens cDNA FLJ14793 fis, clone NT2RP4001174,... weakly similar to NON-GREEN PLASTID TRIOSE PHOSPHATE TRANSLOCATOR PRECURSOR.|PRI PRI 3e-14 +1 ...

  5. GenBank blastx search result: AK059487 [KOME

    Lifescience Database Archive (English)

    Full Text Available g of rodent MAK (male germ cell-associated kinase), the 3' end of the GCMB gene for glial cells missing (Drosophila) homolog b and a CpG island, complete sequence.|PRI PRI 3e-20 +3 ...

  6. GenBank blastx search result: AK111985 [KOME

    Lifescience Database Archive (English)

    Full Text Available g of rodent MAK (male germ cell-associated kinase), the 3' end of the GCMB gene for glial cells missing (Drosophila) homolog b and a CpG island, complete sequence.|PRI PRI 6e-70 +2 ...

  7. GenBank blastx search result: AK104847 [KOME

    Lifescience Database Archive (English)

    Full Text Available g of rodent MAK (male germ cell-associated kinase), the 3' end of the GCMB gene for glial cells missing (Drosophila) homolog b and a CpG island, complete sequence.|PRI PRI 1e-99 +3 ...

  8. GenBank blastx search result: AK064003 [KOME

    Lifescience Database Archive (English)

    Full Text Available some 1 Contains the 5' end of the gene for a novel protein (LOC255104), the gene for a novel protein (FLJ20225), a novel... gene and the 5' end of the gene for a novel protein (KIAA0459), complete sequence.|PRI PRI 6e-22 +2 ...

  9. GenBank blastx search result: AK104725 [KOME

    Lifescience Database Archive (English)

    Full Text Available osome 9p12-13.3 Contains the 3' end of a novel gene (FLJ22611), a novel gene, the GRHPR gene for glyoxylate ...reductase/hydroxypyruvate reductase, a novel gene (KIAA0354), a novel pseuodgene and a CpG island, complete sequence.|PRI PRI 3e-19 +1 ...

  10. GenBank blastx search result: AK104788 [KOME

    Lifescience Database Archive (English)

    Full Text Available osome 9p12-13.3 Contains the 3' end of a novel gene (FLJ22611), a novel gene, the GRHPR gene for glyoxylate ...reductase/hydroxypyruvate reductase, a novel gene (KIAA0354), a novel pseuodgene and a CpG island, complete sequence.|PRI PRI 1e-22 +2 ...

  11. GenBank blastx search result: AK110394 [KOME

    Lifescience Database Archive (English)

    Full Text Available similar to human ceroid-lipofuscinosis, neuronal 2, late infantile(Jansky-Bielschowsky disease) (CLN2), mRNA, RefSeq: NM_000391.2.|PRI PRI 5e-22 +2 ... ...AK110394 002-165-E11 AB169876.1 Macaca fascicularis brain cDNA, clone: QflA-12618,

  12. Teórie etnickej identity Kanchan Chandrovej a národnej indiferencie a možnosti ich uplatnenia pri historickom i etnologickom výskume jazykovo-kultúrne heterogénneho obyvateľstva juhoslovenských regiónov a miest

    Directory of Open Access Journals (Sweden)

    Ondrej Ficeri

    2016-09-01

    Full Text Available Slovak and Hungarian social sciences have paid sufficient attention to research on the transformation of the ethnic identities of people living mainly in ethnically mixed regions and towns of southern Slovakia. In the course of the 20th century, the affected population switched its ethnic identification codes depending on the assimilation political practices or the ethnic policy of the respective state authorities. The aim of this paper is to point out, through the theory of ethnic identity by political scientist Kanchan Chandra from New York (2012, the possibilities of applying an innovative analytical language to the historical and current research of assimilation processes, which enable a more exact grasping of the mechanisms of ethno-cultural changes in the southern Slovakian region heterogenous in terms of language and culture. The inhabitants of this type of regions and towns were easily ethnicised given their potential to become holders of several types of nominal ethnic identities which were activated (assimilation or deactivated (dissimilation depending on the situation in various contexts of the daily public and private life. This “non-national” behaviour of the population (ethnical practice had a causal influence on the current ethnic structure of the “lost” or “recovered” town, which can be interpreted as an expression of national indifference – the concept advocated by social scientists Tara Zahra, Jeremy King or Pieter Judson.

  13. Clinical Observation of the Leonurus artemisia Cream versus Dan’e Fukang Cream in the Treatment of Pri-mary Dysmenorrhea%益母草膏对比丹莪妇康煎膏治疗原发性痛经的临床观察

    Institute of Scientific and Technical Information of China (English)

    李明慧; 芦小娟; 张亚凤; 刘天舒

    2016-01-01

    OBJECTIVE:To compare the efficacy and safety of Leonurus artemisia cream and Dan’e fukang cream in the treat-ment of primary dysmenorrhea. METHODS:80 patients with primary dysmenorrheal were randomly divided into L. artemisia cream group (40 cases) and Dan’e fukang cream group (40 cases). L. artemisia cream group received L. artemisia cream 10 g, orally,twice a day. Dan’e fukang cream group received Dan’e fukang cream 10 g,orally,twice a day. The treatment was stated before 10 days of menstruation. 10 days was regarded as 1 treatment course,and it lasted for 6 courses. Cold drink and food were forbid during medication,washing face and feet with warm water,soaking feet with hot water if they can,paying attention to keep the lower abdomen and legs warm. If patients felt unbearable pain in menstruation,0.3 g Ibuprofen sustained release capsule was given,orally twice a day. Clinical efficacy,symptom score(dysmenorrhea degree,abdominal pain,lumbosacral pain)before and af-ter treatment,dysmenorrhea duration,frequency of taking painkillers and the incidence of adverse reactions in 2 groups were ob-served. RESULTS:There were no significant differences in the total effective rate and the incidence of adverse reactions in 2 groups (P>0.05). Before treatment,there were no significant differences in dysmenorrhea degree,abdominal pain,lumbosacral pain score,dysmenorrheal duration and frequency of taking painkillers in 2 groups (P>0.05). After treatment,the dysmenorrhea degree,abdominal pain,lumbosacral pain score in 2 groups were significantly lower than before,and Dan’e fukang cream group was lower than L. artemisia cream group with statistical sighificance(P0.05). CONCLUSIONS:Both L. artemisia cream and Dan’e fukang cream have good efficacy and safety in the treat-ment of primary dysmenorrhea,while Dan’e fukang cream is superior to L. artemisia cream in terms of improving clinical symp-toms.%目的:比较益母草膏与丹莪妇康煎膏治疗原发性痛经的疗效和安全性。方法:80例原发性痛经患者随机分为益母草膏组(40例)和丹莪妇康煎膏组(40例)。益母草膏组患者给予益母草膏10 g,口服,每日2次。丹莪妇康煎膏组患者给予丹莪妇康煎膏10 g,口服,每日2次。两组患者均自月经前第10天开始用药,连用10 d为1个疗程,共治疗6个疗程。用药期间嘱咐患者不吃冷饮冷食,使用温水洗脸洗脚,有条件者每晚用热水泡脚,注意小腹部和腿部的保暖。若患者行经时疼痛难忍,给予布洛芬缓释胶囊0.3 g,口服,每日2次。观察两组患者的临床疗效,治疗前后症状积分(痛经程度、小腹胀痛、腰骶酸痛)、痛经持续时间、止痛药服用次数及不良反应发生情况。结果:两组患者总有效率、不良反应发生率比较,差异均无统计学意义(P>0.05)。治疗前,两组患者痛经程度积分、小腹胀痛积分、腰骶酸痛积分、痛经持续时间及止痛药服用次数比较,差异均无统计学意义(P>0.05)。治疗后,两组患者痛经程度积分、小腹胀痛积分、腰骶酸痛积分均显著低于同组治疗前,且丹莪妇康煎膏组低于益母草膏组,差异均有统计学意义(P<0.05);痛经持续时间及止痛药服用次数均显著低于同组治疗前,差异有统计学意义(P<0.05),但两组间比较差异无统计学意义(P>0.05)。结论:益母草膏与丹莪妇康煎膏治疗原发性痛经的疗效和安全性均较好,但在改善临床症状方面丹莪妇康煎膏优于益母草膏。

  14. 论商鞅的"同性恋"倾向——窥探电视剧《大秦帝国》第一部%On Shang Yang "Homosexual" Inclination——To Pry Into The Television Series "Da-Qin Empire" (part 1)

    Institute of Scientific and Technical Information of China (English)

    梅进文

    2009-01-01

    电视剧商鞅与秦孝公之间的感情几乎超越了一般意义上的君臣之情,不是简单的说君上对于臣下有赏识重用之恩,使得臣于能是以实现毕生抱负,而臣下自当殚精竭虑,肝脑涂地以报之.在这里我们似乎看到了两个男人在心灵和情感深处的相互依偎与相互扶持,在刚毅的外表下的侠骨柔情.荧玉与白雪从他们各自对于商鞅的感情

  15. ¿Neo o post-neoliberalismo en la política social de México? Una comparación entre las estrategias de política seguidas durante la década del 2000 y la ¿nueva? estrategia del sexenio priísta (2012-2018

    Directory of Open Access Journals (Sweden)

    Katya Rodríguez Gómez

    2014-05-01

    Full Text Available En este artículo se discute el caso de la política social en México para preguntarse si la estrategia de política social que ha sido planteadas en el actual sexenio (2012-2018 constituye un cambio radical respecto a la estrategia de política de la década anterior, y resulta por tanto diferente al modelo de política social neo-liberal, para considerarse políticas pos-neoliberales. O si, por el contrario, no se ha producido ruptura alguna. Para ello, la primera parte discute brevemente las lógicas de política social que pueden situarse en una u otra estrategia. La segunda parte del documento analiza las características de las políticas sociales en México durante el período de 2000 a 2012 bajo los sexenios panistas. La tercera parte del documento describe y analiza el planteamiento de política social y los primeros intentos de implementación de la misma en el sexenio (2012-2018. La última parte responde a la pregunta enunciada.

  16. 'ja hotela skazat eti pustõje slova, govorit, kak oskolki...'; 'oni tantsevali, oni smejalis, u nih bõli bussõ na sheje...'; 'vse mastera, vse kuznetsõ kontshajut rabotu pri utrennem...'; 'u hipohondrika bolit golova, u nego klaustrofobija...' /

    Index Scriptorium Estoniae

    Oidekivi, Kirsti

    1997-01-01

    Orig.: "tahtsin öelda neid tühiseid sõnu, rääkida, kuidas luutükid nahka..."; "nad tantsisid ja naersid, ja helmed olid kaelas..."; "kõik meistrid ja sepistajad lõpetavad töö hommiku- või mõnes muus..."; "hüpohondrikul valutab pea, sest tal on klaustrofoobia..."

  17. 离子束介导大豆DNA的小麦变异株系灌浆后期蛋白水解酶、POD表达分析%Analysis of Proteinases and Pri oxidases in Different Wheat Mutant Lines with Soybean DNA by Ion Beam during Later Grain Filling

    Institute of Scientific and Technical Information of China (English)

    李江伟; 赵一丹; 朱德来; 魏松浩

    2011-01-01

    本研究用蛋白水解酶复性电泳技术,分析了离子束介导大豆DNA的两个高蛋白小麦变异株系和两个低蛋白小麦变异株系旗叶和倒二叶在灌浆后期蛋白水解酶、POD的种类.结果表明,变异株系和对照间以及变异株系间蛋白水解酶、POD的种类和活性存在显著差异;变异株系中检到了一些新的蛋白水解酶带,在pH 5.0条件下变异株系C倒二叶检出220 kD一条新酶带;pH 7.0时变异中性B检到220 kD一条新酶带,株系C中检到260 kD一条新酶带;在pH 8.5时,在变异中A、B、C检到220 kD新酶带,在变异株系B中检到260 kD和280 kD两条新酶带.POD表达酶谱中也发现新带和缺失酶带,变异株系A的旗叶中检到37 kD、39 kD和212kD共3条新带,变异株系B缺失42 kD和47 kD两条酶带.这些新酶带为进一步研究变异株系在DNA水平的差异积累资料.%By renaturation electrophoresis, we analyzed the bands of neutral proteinases and POD isoenzyme in flag leaf and the second leaf above leaf in two high protein and two low protein mutant wheat lines by transferring the total DNA of bean by ion beam. Results showed that there were apparently differences of kinds and activities between wide type and mutant lines. The new proteinase bands had been measured including a 220 kD band in lines C second leaf above at pH 5.0, a 220 kD band in line B and a 260 kD band in line B at pH 7.0. A new band at 220 kD was found in all A, B and C lines, meanwhile a 260 kD and 280 kD new bands was only found in line B. As to POD both new and deletion bands were checked in mutant lines. Three bands at 37 kD, 39 kD and 212 kD were new appearance in line B and two bands at 42 kD and 47 kD were disappeared in line B. Our results are of significance to further research the difference in DNA level in mutant lines.

  18. Zip Codes, U.S. Postal Service zip code boundaries within Sedgwick County. Derived from sczipparc coverage. Each polygon represents one or more contiguous parcels having the same zip code. Base information was provided by U. S. Postal service Airport branch. Pri, Published in 2008, 1:1200 (1in=100ft) scale, Sedgwick County, Kansas.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Zip Codes dataset, published at 1:1200 (1in=100ft) scale, was produced all or in part from Other information as of 2008. It is described as 'U.S. Postal Service...

  19. Percutaneous K-wire pry-poking reposition and fixation for O'Brien Ⅱ, Ⅲ pediatric radial neck fracture%经皮撬拨复位克氏针固定治疗儿童桡骨颈O'BrienⅡ、Ⅲ型骨折

    Institute of Scientific and Technical Information of China (English)

    吴永涛; 尤锐; 姜海; 汪兵; 吴革; 苗武胜

    2015-01-01

    Objective To evaluate the outcome of percutaneous K-wire leverage reduction and fixation for O'Brien Ⅱ,Ⅲ pediatric radial neck fracture.Methods From March 2010 to February 2013,16 children with O'Brien Ⅱ,Ⅲ pediatric radial neck fracture were operated in our hospital.There were 6 females and 10 males aged from 6 to 14 years old.There were 12 left arms and 4 right arms.Concomitant injuries included proximal ulna fractures in 2 cases and medial epicondyle fracture of humerus in 1 case.All children present a swollen and painful elbow with loss of rotation and pain on pronation and supination.The mean visual analogue score (VAS) was 7.9 (range,6.5-9.2).The flextion,extention,pronation and supination of elbow were 78°±3°,-54°±4°,9°±3° and 5°±3° in average.All cases were classified by the O'Brien system,including 9 cases of Ⅱ type and 7 cases of Ⅲ type.Mechanism of injury was fall from a height.The interval between injury and surgery averaged 2.8 days.Percutaneous leverage reduction and fixation were performed for 16 patients.After confirming successful reduction,the wire was advanced to the far cortex of the radius and penetrated into the far cortex to maintain the reduction status.4 weeks of long-arm cast followed by functional exercises were carried out for all patients.We analyzed radiographs and clinical results according to the criteria suggested by Metaizeau.Results 16 patients were followed up for 11.8 month in average after operation.14 patients were gained anatomical reduction.No patient had posterior interosseous nerve injury,fracture redisplacement and pin site infection.All fractures healed in a mean of 4.1 weeks.The follow-up flextion,extention,pronation and supination of elbow were 136°±4°,-2°±2°,81 °±4° and 75°±4° in average.Follow-up radiographs showed no patient had avascular necrosis of radial head.One patient had physeal arrest after 15 months of operation and outpatient follow-up observation were conducted.According to Metaizeau function scale,8 were excellent,7 good and 1 fair.The healing rate was 93.8% (15/16).Conclusion Percutaneous joystick reduction and fixation technique have good effect for O'Brien Ⅱ,Ⅲ pediatric radial neck fracture,which is simple and easy to master.%目的 探讨经皮撬拨复位克氏针固定治疗儿童桡骨颈O'BrienⅡ、Ⅲ型骨折的临床效果.方法 回顾性分析2010年3月至2013年2月治疗的16例桡骨颈O'BrienⅡ、Ⅲ型骨折患儿资料,男10例,女6例;年龄6~ 14岁;左侧12例,右侧4例;均为跌倒致伤.桡骨颈骨折按O'Brien分型:Ⅱ型9例、Ⅲ型7例;2例合并尺骨近端骨折,1例合并肱骨内上髁骨折.患儿临床表现为肘关节外侧肿胀;压痛明显,疼痛视觉模拟评分6.5~ 9.2分,平均7.9分;肘关节活动受限,受伤时肘关节屈曲、伸直、旋前及旋后平均度数为78°±3°、-54°±4°、9°±3°及5°±3°;受伤至手术时间2~4d,平均2.8d.“C”型臂X线机辅助下采用直径2 mm克氏针插入骨折断端撬拨复位,骨折复位后打入撬拨的克氏针.术后长臂石膏托固定肘关节于功能位4周,去石膏托后拔除克氏针行康复训练.结果 16例患儿均获得随访,随访时间6~ 24个月,平均11.8个月;其中14例骨折解剖复位,无一例出现术后复位丢失、骨间背侧神经损伤及针道感染等;手术时间20~ 35 min,平均30 min;骨折均愈合,愈合时间4~6周,平均4.1周.末次随访终时肘关节屈曲、伸直、旋前及旋后平均度数为136°±4°、-2°±2°、81°±4°及75°±4°;无一例合并桡骨头缺血性坏死,1例患儿术后15个月出现桡骨近端骺板早闭,为O'BrienⅢ型桡骨颈骨折,未进一步治疗;根据Metaizeau评估标准,优8例、良7例、可1例;优良率为93.8%(15/16).结论 对于儿童桡骨颈O'BrienⅡ、Ⅲ型骨折,经皮撬拨复位克氏针固定是一种有效的治疗方法,操作简单,疗效肯定.

  20. 丹红注射液治疗ST段抬高型心肌梗死直接经皮冠状动脉介入术后无复流/慢血流的研究%Danhong Injection in Patients with No-reflow/Slow-flow Following Pri-mary PCI of ST-segment Elevation Myocardial Infarction

    Institute of Scientific and Technical Information of China (English)

    杨靖; 崔海明; 任雨笙; 吴宗贵

    2015-01-01

    目的:探讨丹红注射液治疗ST段抬高型心肌梗死( STEMI)直接经皮冠状动脉介入术( PCI)术后无复流/慢血流的有效性、安全性以及机制。方法选择2012年10月-2014年9月罹患STEMI并符合入选标准的患者,随机分配至丹红注射液治疗组和对照组,持续治疗10 d。测定血清心肌坏死标志物、IRA血流速度、血清BNP以及ET-1浓度;记录症状、住院期间病死率和不良反应。结果(1)共入选57例,随机分为丹红组28例,对照组29例,两组间的基线资料无统计学差异;(2)术后3 d心肌坏死标志物峰值,丹红组显著低于对照组;(3)术后10 d IRA的DPV、DMV、DDT≥600 ms比率、STR≥70%的比率以及LVEF,丹红组均显著高于对照组;血清BNP、ET-1水平,丹红组显著低于对照组(;4)住院期间心绞痛/心衰症状发生率,丹红组低于对照组,而病死率两组间比较,差异无统计学意义(;5)丹红组有2例发生轻度副反应。结论丹红注射液能改善直接PCI术后无复流/慢血流的血流速度、减小心梗面积、减轻左室重构及促进左室功能的恢复,该疗效与其降低ET-1水平有关。%Objective To investigate efficacy,safety and mechanism of Danhong Injection( DI)in treatment of patients with no -reflow/ slow -flow phenomenon following primary PCI( PPCI)of STEMI. Methods Patients with STEMI and receiving emergent PPCI in Changzheng hospital from October,2012 to September,2014,meeting inclusion criteria of no-reflow/ slow-flow in infarction related artery(IRA)docu-mented by angiography following PPCI,were assigned randomly to DI group and control group. All patients re-ceived standard treatment,including dual anti-platelet therapy,according to the guideline recommendations. The efficacy endpoints were elevation of serum myocardial biomarkers,restoration of microvascular-myocar-dial perfusion by transthoracic Doppler echocardiography and reservation of LVEF. The safety endpoint in-cluded adverse effect of facial flush,headache,dizziness and palpitation. Serum ET-1 concentrations were tested concurrently. Results A total of 57 patients met inclusion criteria and were assigned to DI group( n=28)and standard therapy(n=29)respectively. There were no significant differences between the two groups at baseline. Peak serum CK,CK-MB and cTnT on day 3 after PPCI were lower in patients received DI than those received standard therapy. DI was observed to increase diastolic peak flow velocity,diastolic mean ve-locity,ratio of diastolic deceleration time≥600 ms,ratio of ST-segment resolution≥70%,as well as LVEF apparently on day 10. Incidence of postinfarction angina and heart failure in hospital,serum BNP and ET-1 were lower in DI group than in control group. Cardiovascular mortality in hospital did not differ significantly between the two groups. Adverse effects of DI were observed rarely and tolerated without drug discontinua-tion. Conclusion In Chinese patients with documented no-reflow/ slow-flow in IRA undergoing PPCI for STEMI,in addition to guideline-recommended therapies,Danhong injection is associated with more restora-tion of compromised blood flow,reduced myocardial infarction size,reservation of left ventricular function and less severity of ventricular remodeling. The beneficial effects could be related to its effect on lowering ET-1level.

  1. Vasodilator-stimulated phosphoprotein-phosphorylation assay in patients on clopidogrel: does standardisation matter?

    Science.gov (United States)

    Freynhofer, Matthias K; Bruno, Veronika; Willheim, Martin; Hübl, Wolfgang; Wojta, Johann; Huber, Kurt

    2012-03-01

    The vasodilator-stimulated phosphoprotein-phosphorylation (VASP-P) flow-cytometric assay is mainly used in clinical trials to measure thienopyridine effects. However, there are remarkable differences in the reported optimal cut-offs, ranging from 48-61% platelet reactivity index (PRI). We therefore investigated whether a lack of standardisation might explain the differences in the cut-offs. We measured VASP-P in 62 individuals. PRI was calculated using the mean, geometric mean and median fluorescence intensities (FI). Stability of the blood-samples (time-to-assay, 0-2 days) and stability of the processed samples (0-120 minutes) within the recommended time-span were tested. Time-to-assay significantly influenced the PRI (pprecise standardisation is inevitable, as the PRI significantly depends on the method of calculation, the time-to-assay as well as on the lag time after processing. Tolerably stable results were obtained for the PRI from the median FI.

  2. Improved plant nitrogen nutrition contributes to higher water use efficiency in tomatoes under alternate partial root-zone irrigation

    DEFF Research Database (Denmark)

    Wang, Yaosheng; Liu, Fulai; Andersen, Mathias Neumann

    2010-01-01

    Comparative effects of partial root-zone irrigation (PRI) and deficit irrigation (DI) on stomatal conductance (gs), nitrogen accumulation and distribution in tomato (Lycopersicon esculentum L.) plants were investigated in a split-root pot experiment. Results showed that both PRI and DI saved 25......% water and led to 10.0% and 17.5% decreases in dry biomass, respectively, compared with the fully irrigated (FI) controls. Consequently, water use efficiency (WUE) was increased by 18.6% and 10.8% in the PRI and DI plants, respectively. The highest WUE in the PRI plants was associated with the highest...... carbon isotope composition (δ13C), indicating that the improvement of WUE might have been a result of long-term optimisation of stomatal control over gas exchange. The constantly higher xylem sap ABA concentration in PRI compared with DI plants was seemingly responsible for the greater control over...

  3. The Effect of Analogues of 1α,25-Dihydroxyvitamin D2 on the Regrowth and Gene Expression of Human Colon Cancer Cells Refractory to 5-Fluorouracil

    Directory of Open Access Journals (Sweden)

    Jacek Neska

    2016-06-01

    Full Text Available This study aimed to evaluate the capacity of hypocalcemic analogues of 1α,25-dihydroxyvitamin D2 (1,25D2 and 1α,25-dihydroxyvitamin D3 (1,25D3 to inhibit regrowth and regulate the stemness-related gene expression in colon cancer cells undergoing renewal after exposure to 5-fluorouracil (5-FU. All of the tested analogues of 1,25D2 equally potently decreased the clonogenicity and the proliferative activity of HT-29 cells which survived the exposure to 5-FU, but differently regulated gene expression of these cells during their renewal. 1,25D2 and analogues (PRI-1907 and PRI-1917, as well as 1,25D3 and analogue PRI-2191, decreased the relative expression level of several stemness-related genes, such as NANOG, OCT3/4, PROM1, SOX2, ALDHA1, CXCR4, in HT-29/5-FU cells during their renewal, in comparison to untreated HT-29/5-FU cells. The other 1,25D2 analogues (PRI-1906 and PRI-1916 were not capable of downregulating the expression of these stemness-related genes as the analogues PRI-1907 and PRI-1917 did. All of the tested vitamin D analogues upregulated CDH1, the gene encoding E-cadherin associated with epithelial phenotype. Out of the series of analogues studied, side-chain branched analogues of 1,25D2 (PRI-1907, PRI-1917 and the analogue of 1,25D3 (PRI-2191 might be used to target cancer cells with stem-like phenotypes that survive conventional chemotherapy.

  4. Dynamics of spectral bio-indicators and their correlations with light use efficiency using directional observations at a Douglas-fir forest

    Science.gov (United States)

    Cheng, Yen-Ben; Middleton, Elizabeth M.; Hilker, Thomas; Coops, Nicholas C.; Black, T. Andrew; Krishnan, Praveena

    2009-09-01

    The carbon science community must rely on satellite remote sensing to obtain global estimates of photosynthetic activity, typically expressed as net primary production (NPP), gross primary production (GPP) or light use efficiency (LUE). The photochemical reflectance index (PRI), calculated as a normalized difference reflectance index using a physiologically active green band (~531 nm) and another physiologically insensitive green reference band (~570 nm), denoted as PRI(570), has been confirmed in many studies as being strongly related to LUE. Here, we examined the potential of utilizing PRI(570) observations under different illumination conditions for canopy LUE estimation of a forest. In order to evaluate this, directional hyperspectral reflectance measurements were collected continuously throughout the daytime periods using an automated spectroradiometer in conjunction with tower-based eddy covariance fluxes and environmental measurements at a coastal conifer forest in British Columbia, Canada throughout the 2006 growing season. A parameter calculated as the PRI(570) difference (dPRI(570)) between shaded versus sunlit canopy foliage sectors showed a strong correlation to tower-based LUE. The seasonal pattern for this correlation produced a dramatic change from high negative (r ~ -0.80) values in the springtime and early fall to high positive values (r ~ 0.80) during the summer months, which could represent the seasonality of physiological characteristics and environmental factors. Although the PRI(570) successfully tracked canopy LUE, one or both of its green bands (~531 and 570 nm) used to calculate the PRI are unavailable on most existing and planned near-term satellites. Therefore, we examined the potential to use 24 other spectral indexes for LUE monitoring that might be correlated to PRI, and thereby a substitute for it. We also continued our previous investigations into the influence of illumination conditions on the observed PRI(570) and other indexes

  5. Microprocessor dynamics and interactions at endogenous imprinted C19MC microRNA genes.

    Science.gov (United States)

    Bellemer, Clément; Bortolin-Cavaillé, Marie-Line; Schmidt, Ute; Jensen, Stig Mølgaard Rask; Kjems, Jørgen; Bertrand, Edouard; Cavaillé, Jérôme

    2012-06-01

    Nuclear primary microRNA (pri-miRNA) processing catalyzed by the DGCR8-Drosha (Microprocessor) complex is highly regulated. Little is known, however, about how microRNA biogenesis is spatially organized within the mammalian nucleus. Here, we image for the first time, in living cells and at the level of a single microRNA cluster, the intranuclear distribution of untagged, endogenously-expressed pri-miRNAs generated at the human imprinted chromosome 19 microRNA cluster (C19MC), from the environment of transcription sites to single molecules of fully released DGCR8-bound pri-miRNAs dispersed throughout the nucleoplasm. We report that a large fraction of Microprocessor concentrates onto unspliced C19MC pri-miRNA deposited in close proximity to their genes. Our live-cell imaging studies provide direct visual evidence that DGCR8 and Drosha are targeted post-transcriptionally to C19MC pri-miRNAs as a preformed complex but dissociate separately. These dynamics support the view that, upon pri-miRNA loading and most probably concomitantly with Drosha-mediated cleavages, Microprocessor undergoes conformational changes that trigger the release of Drosha while DGCR8 remains stably bound to pri-miRNA.

  6. SUMOylation at K707 of DGCR8 controls direct function of primary microRNA

    Science.gov (United States)

    Zhu, Changhong; Chen, Cheng; Huang, Jian; Zhang, Hailong; Zhao, Xian; Deng, Rong; Dou, Jinzhuo; Jin, Hui; Chen, Ran; Xu, Ming; Chen, Qin; Wang, Yanli; Yu, Jianxiu

    2015-01-01

    DGCR8 (DiGeorge syndrome critical region gene 8) is essential for primary microRNA (pri-miRNA) processing in the cell nucleus. It specifically combines with Drosha, a nuclear RNase III enzyme, to form the Microprocessor complex (MC) that cleaves pri-miRNA to precursor miRNA (pre-miRNA), which is further processed to mature miRNA by Dicer, a cytoplasmic RNase III enzyme. Increasing evidences suggest that pri-/pre-miRNAs have direct functions in regulation of gene expression, however the underlying mechanism how it is fine-tuned remains unclear. Here we find that DGCR8 is modified by SUMO1 at the major site K707, which can be promoted by its ERK-activated phosphorylation. SUMOylation of DGCR8 enhances the protein stability by preventing the degradation via the ubiquitin proteasome pathway. More importantly, SUMOylation of DGCR8 does not alter its association with Drosha, the MC activity and miRNA biogenesis, but rather influences its affinity with pri-miRNAs. This altered affinity of DGCR8 with pri-miRNAs seems to control the direct functions of pri-miRNAs in recognition and repression of the target mRNAs, which is evidently linked to the DGCR8 function in regulation of tumorigenesis and cell migration. Collectively, our data suggest a novel mechanism that SUMOylation of DGCR8 controls direct functions of pri-miRNAs in gene silencing. PMID:26202964

  7. Integrating Chlorophyll fapar and Nadir Photochemical Reflectance Index from EO-1/Hyperion to Predict Cornfield Daily Gross Primary Production

    Science.gov (United States)

    Zhang, Qingyuan; Middleton, Elizabeth M.; Cheng, Yen-Ben; Huemmrich, K. Fred; Cook, Bruce D.; Corp, Lawrence A.; Kustas, William P.; Russ, Andrew L.; Prueger, John H.; Yao, Tian

    2016-01-01

    The concept of light use efficiency (Epsilon) and the concept of fraction of photosynthetically active ration (PAR) absorbed for vegetation photosynthesis (PSN), i.e., fAPAR (sub PSN), have been widely utilized to estimate vegetation gross primary productivity (GPP). It has been demonstrated that the photochemical reflectance index (PRI) is empirically related to e. An experimental US Department of Agriculture (USDA) cornfield in Maryland was selected as our study field. We explored the potential of integrating fAPAR(sub chl) (defined as the fraction of PAR absorbed by chlorophyll) and nadir PRI (PRI(sub nadir)) to predict cornfield daily GPP. We acquired nadir or near-nadir EO-1/Hyperion satellite images that covered the cornfield and took nadir in-situ field spectral measurements. Those data were used to derive the PRI(sub nadir) and fAPAR (sub chl). The fAPAR (sub chl) is retrieved with the advanced radiative transfer model PROSAIL2 and the Metropolis approach, a type of Markov Chain Monte Carlo (MCMC) estimation procedure. We define chlorophyll light use efficiency Epsilon (sub chl) as the ratio of vegetation GPP as measured by eddy covariance techniques to PAR absorbed by chlorophyll (Epsilon(sub chl) = GPP/APAR (sub chl). Daily Epsilon (sub chl) retrieved with the EO-1 Hyperion images was regressed with a linear equation of PRI (sub nadir) Epsilon (sub chl) = Alpha × PRI (sub nadir) + Beta). The satellite Epsilon(sub chl- PRI (sub nadir) linear relationship for the cornfield was implemented to develop an integrated daily GPP model [GPP = (Alpha × PRI(sub nadir) + Beta) × fAPAR (sub chl) × PAR], which was evaluated with fAPAR (sub chl) and PRI (sub nadir) retrieved from field measurements. Daily GPP estimated with this fAPAR (sub chl-) PRI (nadir) integration model was strongly correlated with the observed tower in-situ daily GPP (R(sup 2) = 0.93); with a root mean square error (RMSE) of 1.71 g C mol-(sup -1) PPFD and coefficient of variation (CV) of 16

  8. ENHANCEMENT OF RESISTANCE TO OXIDATIVE DEGRADATION OF NATURAL RUBBER THROUGH LATEX DEGRADATION

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    A fully characterised natural rubber latex was subjected to mechanical degradation by stirring at intervals. The resistance to oxidative degradation of the different samples were studied by measuring the Plasticity retention indices (PRI).The results show that there is an enhancement of the PRI from 57% for the undegraded rubber to 79% for the one-hour degraded sample. Further degradation resulted in decrease of PRI as time of degradation increased. Therefore, the one-hour degraded sample is a special rubber with high oxidation resistance which is of great importance in engineering.

  9. Nutritional responses to soil drying and rewetting cycles under partial root-zone drying irrigation

    DEFF Research Database (Denmark)

    Wang, Yaosheng; Jensen, Christian Richardt; Liu, Fulai

    2017-01-01

    signaling that regulates stomatal aperture. PRI induced soil DRW cycles and more soil water dynamics in the root zone enhance soil nutrient mineralization process and thus increase the bioavailability of soil nutrients, resulting in improved nitrogen (N) and phosphorus (P) uptake, in which soil microbial...... processes play a key role. Studies investigating how soil DRW cycles and water dynamics under PRI on nutrient transport in soil solution, soil microbe mediated P transformation, interactions between phytohormones and nutrient uptake, root morphological and architectural traits for nutrient acquisition...... and their bioavailability. Partial root-zone drying irrigation (PRI) irrigates half of the soil zone, while the other half is allowed to dry, and the two halves is alternately irrigated. PRI outweighs conventional deficit irrigation in further improving water use efficiency (WUE) by enhancing the root-to-shoot chemical...

  10. Two-coordinate terminal zinc hydride complexes: synthesis, structure and preliminary reactivity studies.

    Science.gov (United States)

    Dawkins, Michael J C; Middleton, Ewart; Kefalidis, Christos E; Dange, Deepak; Juckel, Martin M; Maron, Laurent; Jones, Cameron

    2016-08-18

    The first examples of essentially two-coordinate, monomeric zinc hydride complexes, LZnH (L = -N(Ar)(SiR3)) (Ar = C6H2{C(H)Ph2}2R'-2,6,4; R = Me, R' = Pr(i) (L'); R = Pr(i), R' = Me (L*); R = Pr(i), R' = Pr(i) (L(†))) have been prepared and shown by crystallographic studies to have near linear N-Zn-H fragments. The results of computational studies imply that any PhZn interactions in the compounds are weak at best. Preliminary reactivity studies reveal the compounds to be effective for the stoichiometric hydrozincation and catalytic hydrosilylation of carbonyl compounds.

  11. GenBank blastx search result: AK241821 [KOME

    Lifescience Database Archive (English)

    Full Text Available dependent regulator of chromatin (subfamily a, member 1), the 5' end of the OCRL gene for oculocerebrorenal syndrome of Lowe and two CpG islands, complete sequence. PRI 5e-41 1 ...

  12. GenBank blastx search result: AK241408 [KOME

    Lifescience Database Archive (English)

    Full Text Available dependent regulator of chromatin (subfamily a, member 1), the 5' end of the OCRL gene for oculocerebrorenal syndrome of Lowe and two CpG islands, complete sequence. PRI 1e-21 1 ...

  13. GenBank blastx search result: AK242828 [KOME

    Lifescience Database Archive (English)

    Full Text Available dependent regulator of chromatin (subfamily a, member 1), the 5' end of the OCRL gene for oculocerebrorenal syndrome of Lowe and two CpG islands, complete sequence. PRI 3e-51 1 ...

  14. GenBank blastx search result: AK242828 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242828 J090067I06 AY204752.1 AY204752 Homo sapiens excision repair cross-complement...ing rodent repair deficiency, complementation group 6 (ERCC6) gene, complete cds. PRI 7e-84 1 ...

  15. GenBank blastx search result: AK242828 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK242828 J090067I06 AB209504.1 AB209504 Homo sapiens mRNA for excision repair cross-complement...ing rodent repair deficiency, complementation group 6 variant protein. PRI 9e-54 1 ...

  16. Experiment list: SRX214068 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available mitive endoderm-like cells || treatment=Treated with ret... musculus; ChIP-Seq source_name=F9+RA cells || cell type=Embryonic carcinoma cell line || developmental stage=Differentiated into pri

  17. Commercialising public research new trends and strategies

    CERN Document Server

    Organisation for Economic Co-operation and Development. Paris

    2013-01-01

    This report describes recent trends in government and institutional level policies to enhance the transfer and exploitation of public research. It also benchmarks a set of countries, universities and public research institutions (PRI) based on both traditional and new indicators.

  18. Een bloemstrook als leverancier van natuurlijke vijanden: Met luzerne minder groene perzikluis op ijsbergsla

    NARCIS (Netherlands)

    Schelling, G.C.; Belder, den E.

    2002-01-01

    PRI onderzocht het effect van stroken bloeiende luzerne tussen vollegronds ijsbergsla op het voorkomen van natuurlijke vijanden van bladluizen. In de luzernestroken bleken vele predatoren en parasieten van bladluizen voor te komen (zweefvliegen; sluipwespen; loopkevers; roofwantsen); met name de aan

  19. Intersectorale samenwerking in de biologische landbouw : mengvoergrondstoffen met binnen- of buitenlandse oorsprong: effect op energieverbruik met mengvoerproductie

    NARCIS (Netherlands)

    Bos, J.F.F.P.

    2006-01-01

    Plant Research International (PRI) gaat in het rapport 'Mengvoergrondstoffen met binnen- of buitenlandse oorsprong: effect op energieverbruik van mengvoerproductie' in op het energieverbruik van de productie van enkele mengvoeders voor biologisch gehouden vleesvarkens. De onderzochte mengvoeders ver

  20. Glomerular filtration rate in patients with gout and factors influencing the rate

    Directory of Open Access Journals (Sweden)

    F M Kudaeva

    2007-09-01

    Zaklyuchenie. Sushchestvuet zavisimost' mezhdu sostoyaniem azotovydelitel'noy funktsii pochek i vozrastom bol'nykh, dlitel'nost'yu i IT podagry. Nalichie AG, IBS, KhSN dostoverno ukhudshaet azotovydelitel'nuyu funktsiyu pochek pri podagre.

  1. Duobla Soleco

    Institute of Scientific and Technical Information of China (English)

    Bai Song

    1996-01-01

    Sinjoro Gao estas altas-tatura, elegante vestita, tamensilentema intelektulo. Atuneli faris la unuan oficvojaonpost sia eklaboro en la institu-cio. Unuflanke li preparis sinpor la vojao, kaj aliflanke limeditis pri si mere:"Kial lakolegoj kromnomas min

  2. Disease: H00945 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available cranial hemorrhages. In plasma, FXIII circulates as a pr...y of the bleeding tendency varies from benign symptoms like excessive bruising to life threatening bleeding emergencies such as intra

  3. Metabolic syndrome as a risk factor for hypertension after preeclampsia

    NARCIS (Netherlands)

    Spaan, J.J.; Sep, S.J.; Balen, V.L. van; Spaanderman, M.E.A.; Peeters, L.L.

    2012-01-01

    OBJECTIVE: To identify metabolic and obstetric risk factors associated with hypertension after preeclampsia. METHODS: We analyzed demographic and clinical data from a postpartum screening (blood pressure, microalbuminuria and fasting plasma levels of glucose, insulin, and lipid profile) from 683 pri

  4. 49 CFR 238.305 - Interior calendar day mechanical inspection of passenger cars.

    Science.gov (United States)

    2010-10-01

    ... paragraphs (c)(8) through (c)(12) and paragraph (d) of this section. (1) All fan openings, exposed gears and..., including a fire extinguisher, pry bar, auxiliary portable lighting, and first aid kits, as applicable,...

  5. Solution Method of Multi-Product Two-Stage Logistics Problem with Constraints of Delivery Course

    Science.gov (United States)

    Ataka, Shinichiro; Gen, Mitsuo

    The logistics network design is one of the important phase of Supply Chain Management (SCM) and it is the problem that should be optimized for long-term promotion of efficiency of the whole supply chain. Usually a plant produces different type of products. Even if it is a factory of the same company, delivery is different by a kind of a produced product. The restrictions which this model has are deeply concerned with TP in the real world. In this paper, we consider the logistics network design problems with multi-products and constraints for delivery course. To solve the problem, we used a hybrid priority-based Genetic Algorithm (h-priGA), and we tried the comparison experiment with priority-based Genetic Algorithm (priGA)and h-priGA, we show it about the effectiveness of h-priGA.

  6. GenBank blastx search result: AK241408 [KOME

    Lifescience Database Archive (English)

    Full Text Available on chromosome Xq13.3-21.2 Contains part of the ATRX gene for alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae), complete sequence. PRI 3e-16 1 ...

  7. Experiment list: SRX514836 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  8. Experiment list: SRX753311 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  9. Experiment list: SRX971581 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  10. Experiment list: SRX099052 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  11. Experiment list: SRX099054 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  12. Experiment list: SRX244149 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  13. Experiment list: SRX244150 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  14. Experiment list: SRX663240 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  15. Experiment list: SRX105185 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  16. Experiment list: SRX323568 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  17. Experiment list: SRX476917 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  18. Experiment list: SRX476927 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  19. Experiment list: SRX476918 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  20. Experiment list: SRX476928 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available =Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers... of the keratinocytes forming the epidermal layers are basal cell, spinous or pri

  1. Incidence and risk factors of occupational blood exposure: a nation-wide survey among Danish doctors

    DEFF Research Database (Denmark)

    Nelsing, S; Nielsen, T L; Brønnum-Hansen, H;

    1997-01-01

    Occupational blood exposures involves a risk of transmission of serious infections. We performed a nation-wide survey, to describe the incidence and risk factors of percutaneous (PCE) and mucocutaneous (MCE) blood exposures among hospital employed doctors in Denmark. Of 9,374 questionnaires, 6.......6-3.1 PCE/pry and 6.0-6.9 MCE/pry). Finally Pathology, Internal medicine, Radiology and Paediatrics had a considerable risk (0.8-1.3 PCE/pry and 1.3-2.9 MCE/pry). Potential risk factors were examined by Poisson regression. Employment as senior as compared to junior doctor was associated with a higher risk...... doctors. Non-compliance with UP was associated with an increased risk of exposure and efforts to improve compliance with UP as well as implementation and evaluation of other preventive measures are needed....

  2. Kiam bestoj parolas

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Ofte oni bezonas scii, kiel nomi la "lingvajon" de la bestoj... sed mankas vortoj! Jen listo dekelkaj utilaj onomatopeoj. cu eble vi scias pri pliaj, kiujn ni povus aldoni al gi? Skribu al laredakcio!

  3. Uudised : Balleti võlumaailm. Agent M sai maha uue plaadi, singli ja videoga

    Index Scriptorium Estoniae

    2007-01-01

    Rahvusooper Estonia, eesotsas Mai Murdmaa, Tatjana Järvi ja Lemme Järviga, korraldab põhikoolinoortele balletti tutvustavaid etendusi. Alternatiivrokki viljeleva ansambli Agent M heliplaadist "Spionaazh" ja videost laulule "Queen of the World" (www.agentm.pri.ee)

  4. Tatiana Detlofson: "Mandariinide" film on nagu väike kalliskivi üüratus meres / intervjueerinud Annela Laaneots

    Index Scriptorium Estoniae

    Detlofson, Tatiana

    2016-01-01

    Intervjuu Oscari nominatsiooni jõudnud Eesti filmi "Mandariinid" PR-tegija Tatiana Detlofsoniga filmi PR-i eduteguritest. Filmi meeskond pälvis Aasta Suhtekorraldaja 2015 tiitli professionaalse ja tulemusliku PR-töö eest

  5. Relationship between photochemical efficiency of photosystem II and the photochemical reflectance index of mango tree: merging data from different illuminations, seasons and leaf colors.

    Science.gov (United States)

    Weng, Jen-Hsien; Jhaung, Lee-Hau; Lin, Rong-Jhih; Chen, Hong-Yi

    2010-04-01

    In order to elucidate the effects of chlorophyll concentration and seasonal temperature on the relationship between photosystem II (PSII) efficiency and the photochemical reflectance index (PRI) of leaves under different light intensity, mango (Mangifera indica), a low-temperature-sensitive species, was used for the study. From early winter to summer, we selected several days to measure chlorophyll fluorescence and leaf spectral reflectance of mango leaves with dark green to yellow green colors, under natural sunlight from predawn to sunset and under six levels (0, 200, 400, 800, 1200 and 2000 mumol m(-2) s(-1)) of artificial illumination. When leaves were exposed to light, both PRI and PSII efficiency decreased with the increase in illumination, yet the PSII efficiency-PRI relationship varied with temperature and leaf color. Both predawn PRI and the X-intercept of the PSII efficiency-PRI regression equations were higher in dark green leaves and on the day with higher minimum air temperature, and lower on opposite conditions. These were due to the influence of chlorophyll on the reflection of wavebands for detecting PRI, and leaves retained a higher degree of epoxidation state of xanthophyll cycle pigments in cold predawn. Therefore, when data obtained at different seasons and with different leaf colors were pooled for analysis, PRI was not closely related to PSII efficiency. Yet, either in the darkness of predawn or under a given level of illumination, PSII efficiency always showed a significant positive correlation with PRI, with data from different leaf colors and seasons merged for statistics analysis. Because both the intercept and slope of the PSII efficiency-PRI equation showed a negative regression with photosynthetic photon flux (PPF), an empirical regression model, i.e., PSII efficiency = c + d . PPF + e . (PPF)(2) + f . PRI + g . PPF . PRI, could be fitted for multiple regression analysis. Based on the close correlation between the estimated and measured

  6. OPTIMIZACIJA POSLOVANJA V MATERIAL MANAGEMENTU SCHWARZKOPF HENKEL

    OpenAIRE

    Rogič, Luka

    2016-01-01

    V diplomskem delu na primeru podjetja Schwarzkopf Henkel predstavljamo strategije optimizacije material managementa. Pri tem se osredotočamo na strategije offshoringa, v okviru katerih podjetje v svoji oskrbovalni verigi vzpostavlja skupne storitvene centre ter pristojbinske pogodbene proizvajalce. V diplomski nalogi obravnavamo vzpostavitev tehnično kompetentnega centra v Mariboru in vzpostavitev pristojbinskega pogodbenega izvajalca na Slovaškem. Pri tem ugotavljamo posamezne korake v proce...

  7. Photoperiod and temperature constraints on the relationship between the photochemical reflectance index and the light use efficiency of photosynthesis in Pinus strobus.

    Science.gov (United States)

    Fréchette, Emmanuelle; Chang, Christine Yao-Yun; Ensminger, Ingo

    2016-03-01

    The photochemical reflectance index (PRI) is a proxy for the activity of the photoprotective xanthophyll cycle and photosynthetic light use efficiency (LUE) in plants. Evergreen conifers downregulate photosynthesis in autumn in response to low temperature and shorter photoperiod, and the dynamic xanthophyll cycle-mediated non-photochemical quenching (NPQ) is replaced by sustained NPQ. We hypothesized that this shift in xanthophyll cycle-dependent energy partitioning during the autumn is the cause for variations in the PRI-LUE relationship. In order to test our hypothesis, we characterized energy partitioning and pigment composition during a simulated summer-autumn transition in a conifer and assessed the effects of temperature and photoperiod on the PRI-LUE relationship. We measured gas exchange, chlorophyll fluorescence and leaf reflectance during the photosynthetic downregulation in Pinus strobus L. seedlings exposed to low temperature/short photoperiod or elevated temperature/short photoperiod conditions. Shifts in energy partitioning during simulated autumn were observed when the pools of chlorophylls decreased and pools of photoprotective carotenoids increased. On a seasonal timescale, PRI was controlled by carotenoid pool sizes rather than xanthophyll cycle dynamics. Photochemical reflectance index variation under cold autumn conditions mainly reflected long-term pigment pool adjustments associated with sustained NPQ, which impaired the PRI-LUE relationship. Exposure to warm autumn conditions prevented the induction of sustained NPQ but still impaired the PRI-LUE relationship. We therefore conclude that alternative zeaxanthin-independent NPQ mechanisms, which remain undetected by the PRI, are present under both cold and warm autumn conditions, contributing to the discrepancy in the PRI-LUE relationship during autumn.

  8. Oscillation of a rigid rod in the special relativity

    CERN Document Server

    Paiva, F M

    2012-01-01

    In the special relativity, a rigid rod slides upon itself, with one extremity oscillating harmonically. We discovered restrictions in the amplitude of the motion and in the length of the rod, essential to eliminate unphysical solutions. ------- Cxe la speciala relativeco, rigida stango movigxas sur si mem, kun unu fino oscilante harmonie. Ni malkovris limigajn kondicxojn pri la amplitudo de movado kaj pri la longo de stango, necesegaj por elimini ne-fizikajn solvojn.

  9. JAVASCRIPT OGRODJE JQUERY

    OpenAIRE

    Lemajič, Jani

    2011-01-01

    V diplomskem delu se spoznavamo z JavaScript ogrodjem jQuery in njegovo uporabo. Podanih je nekaj primerov uporabe ogrodja jQuery v praksi tako pri izdelavi spletnih aplikacij, kot pri oblikovanju izgleda spletne strani. Opisane so tudi ostale tehnologije, ki so povezane z izdelavo spletnih aplikacij z ogrodjem jQuery. Izdelali smo primerjavo ogrodja jQuery s podobnim JavaScript ogrodjem MooTools, ter poiskali njune prednosti in slabosti.

  10. ANALIZA IN UPORABA ORODIJ CAAT ZA REVIZIJO INFORMACIJSKIH SISTEMOV

    OpenAIRE

    2010-01-01

    V diplomski nalogi smo predstavili in uporabili orodja CAAT, ki služijo kot pomoč pri revidiranju informacijskih sistemov. Predstavili smo tri orodja, to so IDEA, TopCAATs in ACL. Povzeli smo njihove temeljne funkcije, ki so uporabne pri revidiranju. V nadaljevanju smo sestavili testni primer na katerem smo preizkusili vsako izmed omenjenih orodij. Tako smo ugotovili prednosti in slabosti teh orodij na sploh, ter za vsakega posebej.

  11. 原发性失眠症患者人格发展异常过程分析--基于广安门忆溯性人格量表(GMPI)数据的分析%Analysis of the Abnor mal Personality Develop ment of Pri mary Inso mnia-Analysis based on Da-ta of Guangan Men Me mory-tracing Personality Inventory (GMPI)

    Institute of Scientific and Technical Information of China (English)

    吕学玉; 周璇梓; 李桂侠; 李婧; 单志燕; 栗晓霞; 汪卫东

    2014-01-01

    Objective To analyze the abnormal personality development of primary insomnia.Methods to investi-gate the patients and healthy controls by GMPI,and to find the differences and regularity.Results Differences in three periods of patients are various:during childhood,the differences are relatively minor,which manifested in lower autonomylevel in primary insomnia;as growing up,more deficits in personality development of primary insomnia appear,which mainly resides in abnormal thinking mode,excessive social phobia,and alower autonomy level (P <0.05 or 0.01).Those who live in different times manifest their own developmental characteristics.In childhood,those younger than 36 years old developed obvious differently from those who older than 50 years old (P <0.05 or 0.01),while those age between them did notmanifest such obvious regularity among them;as in the second developmental period,the trend continued.However,in certain dimensions,patients whose age between 36 and 50 years turned out to be develop differently than those older than 50 years old (P <0.05 or 0.01),especially inparent-child relationship dimensions and autonomy dimensions.Generally,patients younger than 36 years old had lower developmental level in autonomy and emotions dimensions.Particularly,they had superior developmental level in career value.Conclusion Differences in personality developmental level between primary insomnia and healthy controls are found;what is more,the differences have brand of times.%目的:分析原发性失眠症患者人格发展异常过程。方法运用 GMPI 对原发性失眠症患者与健康人进行调查,分析2组人群差异及发现某些规律。结果失眠患者与健康人在三个年龄段存在的差异不同,其中童年期的差异尚小,仅仅表现于自主性发展水平弱于健康人群;随着年龄的增长,失眠症患者表现出越来越多的人格发展不足,重点表现在思维方式、社会恐惧感、自主性三个方面(P <0.05或0.01);不同年龄的患者在三个阶段表现出不同的发展特点。上小学之前,小于36岁的患者与大于50岁的患者表现出较明显的差异(P <0.05或0.01),但36~50岁之间的患者未发现与这2组患者的明显差异;在上小学至18岁这个年龄段的人格发展方面,继续保持这一趋势,但在这个阶段,在某些方面,小于36岁的患者组与36~50岁之间的患者组的差异也渐趋明显,尤其体现在不良教养方式以及社会自我、心理自我、独立性及自主性等方面。普遍的,小于36岁的患者组失眠症患者在自我分量表以及情绪情感分量表表现出的发展水平较低,而特殊的,在事业观维度,小于36岁的患者组表现出较高的发展水平。结论原发性失眠症患者与健康人的人格发展过程存在差异,而且具有时代烙印。

  12. Vitamin D Analogs Potentiate the Antitumor Effect of Imatinib Mesylate in a Human A549 Lung Tumor Model.

    Science.gov (United States)

    Maj, Ewa; Filip-Psurska, Beata; Świtalska, Marta; Kutner, Andrzej; Wietrzyk, Joanna

    2015-11-13

    In previous papers, we presented data on studies on the anticancer activity of the vitamin D₃ analogs, named PRI-2191 and PRI-2205, in different cancer models. In this study, we showed the improved antiproliferative activity of a combination of imatinib mesylate (Gleevec, GV) and cytostatic agents in in vitro studies, when used with a third compound, namely PRI-2191, in an A549 human lung cancer model. Furthermore, we analyzed the influence of both PRI-2191, as well as PRI-2205 on the anticancer activity of GV in mice bearing A549 tumors. The route of PRI-2191 analog administration showed a significant impact on the outcome of GV treatment: subcutaneous injection was more efficient and less toxic than oral gavage. Moreover, both vitamin D compounds increased the anticancer activity of GV; however, they might also potentiate some adverse effects. We also evaluated in tumor tissue the expression of VEGF, PDGF-BB, vitamin D receptor, CYP27B1, CYP24, p53 and Bcl-2, as well as PDGF receptors: α and β. We observed the upregulation of p53 expression and the downregulation of Bcl-2, as well as VEGF in A549 tumors as a result of the tested treatment. However, vitamin D analogs did not significantly influence the expression of these proteins.

  13. Vitamin D Analogs Potentiate the Antitumor Effect of Imatinib Mesylate in a Human A549 Lung Tumor Model

    Science.gov (United States)

    Maj, Ewa; Filip-Psurska, Beata; Świtalska, Marta; Kutner, Andrzej; Wietrzyk, Joanna

    2015-01-01

    In previous papers, we presented data on studies on the anticancer activity of the vitamin D3 analogs, named PRI-2191 and PRI-2205, in different cancer models. In this study, we showed the improved antiproliferative activity of a combination of imatinib mesylate (Gleevec, GV) and cytostatic agents in in vitro studies, when used with a third compound, namely PRI-2191, in an A549 human lung cancer model. Furthermore, we analyzed the influence of both PRI-2191, as well as PRI-2205 on the anticancer activity of GV in mice bearing A549 tumors. The route of PRI-2191 analog administration showed a significant impact on the outcome of GV treatment: subcutaneous injection was more efficient and less toxic than oral gavage. Moreover, both vitamin D compounds increased the anticancer activity of GV; however, they might also potentiate some adverse effects. We also evaluated in tumor tissue the expression of VEGF, PDGF-BB, vitamin D receptor, CYP27B1, CYP24, p53 and Bcl-2, as well as PDGF receptors: α and β. We observed the upregulation of p53 expression and the downregulation of Bcl-2, as well as VEGF in A549 tumors as a result of the tested treatment. However, vitamin D analogs did not significantly influence the expression of these proteins. PMID:26580599

  14. Vitamin D Analogs Potentiate the Antitumor Effect of Imatinib Mesylate in a Human A549 Lung Tumor Model

    Directory of Open Access Journals (Sweden)

    Ewa Maj

    2015-11-01

    Full Text Available In previous papers, we presented data on studies on the anticancer activity of the vitamin D3 analogs, named PRI-2191 and PRI-2205, in different cancer models. In this study, we showed the improved antiproliferative activity of a combination of imatinib mesylate (Gleevec, GV and cytostatic agents in in vitro studies, when used with a third compound, namely PRI-2191, in an A549 human lung cancer model. Furthermore, we analyzed the influence of both PRI-2191, as well as PRI-2205 on the anticancer activity of GV in mice bearing A549 tumors. The route of PRI-2191 analog administration showed a significant impact on the outcome of GV treatment: subcutaneous injection was more efficient and less toxic than oral gavage. Moreover, both vitamin D compounds increased the anticancer activity of GV; however, they might also potentiate some adverse effects. We also evaluated in tumor tissue the expression of VEGF, PDGF-BB, vitamin D receptor, CYP27B1, CYP24, p53 and Bcl-2, as well as PDGF receptors: α and β. We observed the upregulation of p53 expression and the downregulation of Bcl-2, as well as VEGF in A549 tumors as a result of the tested treatment. However, vitamin D analogs did not significantly influence the expression of these proteins.

  15. Improved Algorithm on Radar Pulse Signals’Deinterleaving%一种改进的雷达脉冲信号分选算法

    Institute of Scientific and Technical Information of China (English)

    李永红; 邱杰; 孙文军

    2011-01-01

    重频分选方法中,由于序列差值直方图法(SDIF)对PRI抖动的雷达脉冲信号的分选能力差,而PRI变换法可以分选PRI抖动的雷达脉冲信号,但是运算量太大,难以实现实时分选。对SDIF算法进行了改进,采用交叠PRI箱的方法进行直方图的统计,使得改进后的SDIF在运算量增加不多的情况下,可以实时较好地分选PRI抖动量在10%以内的雷达脉冲信号。%Among the current PRI sorting algorithms,the sequential difference histogram algorithm(SDIF) can’t deinterleave the radar signals with PRI jitter perfectly,while the PRI transform algorithm can deinterleave the PRI jittered radar signals but can’t realize in real time due to its huge computing cost.Therefore,the SDIF algorithm is improved by using overlapped PRI bins in histogram statistics.The im-proved SDIF algorithm doesn’t increase the amount of calculation a lot with respect to the original SDIF al-gorithm,and can well deinterleave the PRI jittered radar signals with jitter quantity within 10%.

  16. Unified Field Theory and Principle of Representation Invariance

    CERN Document Server

    Ma, Tian

    2012-01-01

    This is part of a research program to establish a unified field model for interactions in nature. The aim of this article is to postulate a new principle of representation invariance (PRI), to provide a needed mathematical foundation for PRI, and to use PRI to refine the unified field equations of four interactions. Intuitively, PRI amounts to saying that all SU(N) gauge theories should be invariant under transformations of different representations of SU(N). With PRI, we are able to substantially reduce the number of to-be-determined parameters in the unified model to two SU(2) and SU(3) constant vectors $\\{\\alpha^1_\\mu \\}$ and $\\{\\alpha^2_k\\}$, containing 11 parameters, which represent the portions distributed to the gauge potentials by the weak and strong charges. Furthermore, both PRI and PID can be directly applied to individual interactions, leading to a unified theory for dark matter and dark energy, and theories on strong and weak interaction potentials. As a direct application of the strong interacti...

  17. Monitoring water stress and fruit quality in an orange orchard under regulated deficit irrigation using narrow-band structural and physiological remote sensing indices

    Science.gov (United States)

    Stagakis, S.; González-Dugo, V.; Cid, P.; Guillén-Climent, M. L.; Zarco-Tejada, P. J.

    2012-07-01

    This paper deals with the monitoring of water status and the assessment of the effect of stress on citrus fruit quality using structural and physiological remote sensing indices. Four flights were conducted over a citrus orchard in 2009 using an unmanned aerial vehicle (UAV) carrying a multispectral camera with six narrow spectral bands in the visible and near infrared. Physiological indices such as the Photochemical Reflectance Index (PRI570), a new structurally robust PRI formulation that uses the 515 nm as the reference band (PRI515), and a chlorophyll ratio (R700/R670) were compared against the Normalized Difference Vegetation Index (NDVI), Renormalized Difference Vegetation Index (RDVI) and Modified Triangular Vegetation Index (MTVI) canopy structural indices for their performance in tracking water status and the effects of sustained water stress on fruit quality at harvest. The irrigation setup in the commercial orchard was compared against a treatment scheduled to satisfy full requirements (based on estimated crop evapotranspiration) using two regulated deficit irrigation (RDI) strategies. The water status of the trees throughout the experiment was monitored with frequent field measurements of stem water potential (Ψx), while titratable acidity (TA) and total soluble solids (TSS) were measured at harvest on selected trees from each irrigation treatment. The high spatial resolution of the multispectral imagery (30 cm pixel size) enabled identification of pure tree crown components, extracting the tree reflectance from shaded, sunlit and aggregated pixels. The physiological and structural indices were then calculated from each tree at the following levels: (i) pure sunlit tree crown, (ii) entire crown, aggregating the within-crown shadows, and (iii) simulating a lower resolution pixel, including tree crown, sunlit and shaded soil pixels. The resulting analysis demonstrated that both PRI formulations were able to track water status, except when water stress

  18. Conservation measures undertaken to improve the population status of eastern imperial eagle (Aquila heliaca in Bulgaria

    Directory of Open Access Journals (Sweden)

    Demerdzhiev Dimitar

    2014-01-01

    Full Text Available Štúdia prezentuje výsledky ochranárskych opatrení a ich vplyv na populáciu orla královského v Bulharsku. Druh sa vyskytuje v juhovýchodnej casti krajiny, v centrálnom Bulharsku je známy len jeden pár. V období 2009-201 3 bolo obsadených 28 rôznych hniezdnych teritórií. Populácia narástla o 20 %, pocet párov s hniezdnymi teritóriami sa zvýšil z 20 v roku 2009 na 24 v roku 201 3. Stredné hodnoty parametrov hniezdenia orlov královských boli v období 2009-201 3 nasledovné: produktivita (P 1 ,03 ± 0,1 8; hniezdna úspešnost (BS 1 ,1 8±0,21 , úspešnost vyletenia mládat (FS 1 .68±0,1 6. Rocná miera prežitia hniezdiacich jedincov bola 0,795-0,932. Najdôležitejším faktorom, spôsobujúcim mortalitu mladých orlov boli usmrtenia po zásahu elektrickým prúdom spolocne s nelegálnymi odstrelmi a otravami. Strážené páry mali vyššiu hniezdnu úspešnost (1 ,32±0,88 ako nestrážené páry (1 ,06±0,91 . Celkom bolo obsadených vtákmi rôznych druhov 39 % umelých hniezd inštalovaných v období 2009-201 3. V období 2008-201 3 bolo celkom 23 mladých orlov královských oznacených satelitnými vysielackami s GPS jednotkou. V teritóriách siedmich párov bolo izolovaných 608 nebezpecných stlpov elektrického vedenia. V jesennom a zimnom období (október až marec sa raz za týžden realizovalo prikrmovanie 1 4 párov orlov. Celkom sa zrealizovalo 483 prikrmovaní, na 450 z nich bolo súcastou sledovanie efektivity tejto cinnosti.

  19. The core microprocessor component DiGeorge syndrome critical region 8 (DGCR8) is a nonspecific RNA-binding protein.

    Science.gov (United States)

    Roth, Braden M; Ishimaru, Daniella; Hennig, Mirko

    2013-09-13

    MicroRNA (miRNA) biogenesis follows a conserved succession of processing steps, beginning with the recognition and liberation of an miRNA-containing precursor miRNA hairpin from a large primary miRNA transcript (pri-miRNA) by the Microprocessor, which consists of the nuclear RNase III Drosha and the double-stranded RNA-binding domain protein DGCR8 (DiGeorge syndrome critical region protein 8). Current models suggest that specific recognition is driven by DGCR8 detection of single-stranded elements of the pri-miRNA stem-loop followed by Drosha recruitment and pri-miRNA cleavage. Because countless RNA transcripts feature single-stranded-dsRNA junctions and DGCR8 can bind hundreds of mRNAs, we explored correlations between RNA binding properties of DGCR8 and specific pri-miRNA substrate processing. We found that DGCR8 bound single-stranded, double-stranded, and random hairpin transcripts with similar affinity. Further investigation of DGCR8/pri-mir-16 interactions by NMR detected intermediate exchange regimes over a wide range of stoichiometric ratios. Diffusion analysis of DGCR8/pri-mir-16 interactions by pulsed field gradient NMR lent further support to dynamic complex formation involving free components in exchange with complexes of varying stoichiometry, although in vitro processing assays showed exclusive cleavage of pri-mir-16 variants bearing single-stranded flanking regions. Our results indicate that DGCR8 binds RNA nonspecifically. Therefore, a sequential model of DGCR8 recognition followed by Drosha recruitment is unlikely. Known RNA substrate requirements are broad and include 70-nucleotide hairpins with unpaired flanking regions. Thus, specific RNA processing is likely facilitated by preformed DGCR8-Drosha heterodimers that can discriminate between authentic substrates and other hairpins.

  20. Successful disabling of the 5' UTR of HCV using adeno-associated viral vectors to deliver modular multimeric primary microRNA mimics.

    Science.gov (United States)

    Bourhill, Tarryn; Arbuthnot, Patrick; Ely, Abdullah

    2016-09-01

    Chronic hepatitis C virus (HCV) infection is a major health concern and is strongly associated with cirrhosis, hepatocellular carcinoma and liver-related mortality. The HCV genome is the template for both protein translation and viral replication and, being RNA, is amenable to direct genetic silencing by RNA interference (RNAi). HCV is a highly mutable virus and is capable of escaping RNAi-mediated silencing. This has highlighted the importance of developing RNAi-based therapy that simultaneously targets multiple regions of the HCV genome. To develop a multi-targeting RNAi activator, a novel approach for the generation of anti-HCV gene therapy was investigated. Five artificial primary miRNA (pri-miR) were each designed to mimic the naturally occurring monomeric pri-miR-31. Potent knockdown of an HCV reporter was seen with four of the five constructs and were processed according to the intended design. The design of the individual pri-miR mimics enabled the modular assembly into multimeric mimics of any possible conformation. Consequently the four potent pri-miR mimics were used to generate polycistronic cassettes, which showed impressive silencing of an HCV target. To further their application as a gene therapy, recombinant adeno-associated viral (rAAV) vectors that express the polycistronic pri-miR mimics were generated. All AAV-delivered anti-HCV pri-miR mimics significantly knocked down the expression of an HCV target and showed inhibition of HCV replicon replication. Here we describe a protocol for the generation of therapeutic rAAVs that express modular polycistronic pri-miR cassettes allowing for rapid alteration and generation of tailored therapeutic constructs against HCV.