WorldWideScience

Sample records for ciliary body melanocytoma

  1. Pseudotumor of Ciliary Body

    Directory of Open Access Journals (Sweden)

    Mary Varghese

    2014-01-01

    Full Text Available Orbital pseudotumor is a benign disease involving the orbital structures. Pseudotumor of the ciliary body is rare. We present a case of a 27-year-old male who presented with gradual visual loss, pain, and redness in his left eye. On examination he was found to have a yellowish white mass at the periphery of anterior chamber in his left eye and ultrasound biomicroscopy (UBM revealed a ciliary body mass in the same eye. He was treated with systemic steroids, which was tapered over a period of 8 weeks. His symptoms improved and the ciliary body mass disappeared with no recurrence over the next 6 months. UBM is an important diagnostic tool for diagnosing ciliary body mass. Early diagnosis and prompt treatment with systemic steroids may help resolve pseudotumor of the ciliary body.

  2. Local excision of ciliary body tumors:a long-term prospective cohort study in China

    Institute of Scientific and Technical Information of China (English)

    WEI Wen-bin; YANG Wen-li; HU Shi-min; LI Bin

    2008-01-01

    Background Ciliary body tumors are usually difficult to diagnose and treat in early stages.However,treatment of such tumors has trended toward ocular conservation instead of enucleation.Local excision of ciliary body tumors has become effective with the development of the modern vitrectomy,but long-term outcomes are still not clear.Therefore,we reported the outcome of locally excised ciliary body tumors after long-term follow-up.Methods Twenty-two patients (22 eyes),who had been diagnosed with ciliary body tumors in Beijing Tongren Hospital from January 1996 to June 2001,were enrolled in this prospective cohort study.Localized lamellar sclera-ciliary excisions were performed.In some cases,vitrectomies,scleral graft transplantations,and further excisions of the anterior choroid were performed.Diagnoses were confirmed by histopathologic examination.Patients were followed from five to eleven years after surgery.Their visual acuity,intraocular pressure and local recurrence were recorded with descriptive percentages.Results After surgery,all patients maintained normal eyeball appearances.Six patients maintained circular pupils.The final best corrected visual acuities (BCVA) varied from 0.02-1.00,including 18 patients (81.82%) who had BCVA equal to or better than that before surgery.Fifteen patients (68.18%) had BCVA better than 0.3.Only two patients had intraocular pressure (IOP) of less than 10 mmHg and the other patients maintained normal IOP.Nine cases (40.91%) were given confirmed diagnosis of malignant melanoma,four (18.18%) of melanocytoma,six (27.27%) of nonpigmented ciliary epithelial adenoma,two (9.09%) of neurofibroma,and one (4.55%) of neurinoma.Twenty patients (90.91%) had no recurrence during the follow-up period.In one case melanocytoma recurred seven years after surgery and enucleation was performed.One patient,whose operation removed a malignant melanoma with a diameter of 16 mm,died of hepatic metastasis five years after the operation.Conclusion For

  3. Primary Spinal Meningeal Melanocytoma

    Energy Technology Data Exchange (ETDEWEB)

    Ha, Dong Ho [Dong-A University, Busan (Korea, Republic of)

    2009-12-15

    Primary meningeal melanocytic neoplasms are rare lesions that originate from leptomeningeal melanocytes. An intradural meningeal melanocytoma in the thoracic spine is less common than a malignant melanoma, which is its malignant counterpart. We report a case of a histopathologically confirmed primary intradural meningeal melanocytoma in the thoracic spine along with a literature.

  4. The Pediatric Choroidal and Ciliary Body Melanoma Study

    DEFF Research Database (Denmark)

    Al-Jamal, Rana'a T; Cassoux, Nathalie; Desjardins, Laurence;

    2016-01-01

    PURPOSE: To collect comprehensive data on choroidal and ciliary body melanoma (CCBM) in children and to validate hypotheses regarding pediatric CCBM: children younger than 18 years, males, and those without ciliary body involvement (CBI) have more favorable survival prognosis than young adults 18...

  5. Angioleiomyoma of the Ciliary Body:A Case Report

    Institute of Scientific and Technical Information of China (English)

    Jianhua Yan; Zhongyao Wu; Yongping Li; Guanguang Feng; Hao Zhang

    2004-01-01

    Purpose: To report a rare case of angioleiomyoma of the ciliary body Methods :The clinical manifestation, imaging findings, histopathologic characteristics were analyzed in a 32-year-old male patient with angioleiomyoma of the ciliary body. Results:The tumor was removed intact with local resection. Histopathologic examination revealed that the tumor was full of vessels and it was composed of spindle cells with abundant cytoplasm. Immunohistochemical studies showed positive for SMA and Desmin and negative for S100 and HMB-45. Conclusions: Angioleiomyoma of the ciliary body is a rare tumor that can be successfully treated with local surgical resection in this area. It needs to be differentiated from other tumors, especially malignant melanoma. Eye Science 2004;20:19-22.

  6. Progressive hemifacial atrophy with ciliary body atrophy and ocular hypotony

    Directory of Open Access Journals (Sweden)

    T Ashwini Kini

    2015-01-01

    Full Text Available Progressive hemifacial atrophy (PHA is a disease of unknown etiology affecting one-half of the face. Ocular involvement is uncommon. Atrophy of iris is rare, with only a few cases of partial atrophy being reported in the literature. We report a case of total atrophy of iris and ciliary body with associated ocular hypotony in a 16-year-old girl with PHA. We believe this is the first reported case of complete atrophy of iris and ciliary body in PHA. Ocular hypotony in PHA was thought to be due to intra-ocular inflammation. However in our case it appears to be secondary to severe atrophy of the ciliary body.

  7. Multicavitary ciliary body melanoma presenting as a cyst

    Directory of Open Access Journals (Sweden)

    Jennifer Jang

    2013-01-01

    Full Text Available Cyst-like cavities in uveal melanoma occur rarely and can simulate a benign intraocular cystic lesion resulting in delayed diagnosis and inappropriate management. Herein, we describe a 66-year-old Caucasian female who presented with a "cystic" ciliary body mass in the right eye oculus dexter (OD. Slit lamp examination OD showed anterior bulging of the iris temporally from an underlying pigmented ciliary body mass and transillumination disclosed slight shadow from the tumor. Ultrasound biomicroscopy (UBM revealed multiple cyst-like cavities within a tumor, lined by "thick walls" of at least 200 μm and occupying 80% of the tumor volume. A clinical diagnosis of multi-cavitary ciliary body melanoma was suspected and partial lamellar sclero iridocyclectomy was performed. Histopathology confirmed the diagnosis of low-grade spindle melanoma of the ciliary body with multiple empty and fluid filled cyst-like cavities without epithelial lining. UBM is an important diagnostic tool in the differentiation of "thick walled" cavitary melanoma from "thin walled" benign pigment epithelial cyst.

  8. Expression of nitric oxide synthase and guanylate cyclase in the human ciliary body and trabecular meshwork

    Institute of Scientific and Technical Information of China (English)

    WU Ren-yi; MA Ning

    2012-01-01

    Background The role played by the nitric oxide (NO) signaling pathway in the aqueous humor dynamics is still unclear.This study was designed to investigate the expression and distribution of NO synthase (NOS) isoforms and guanylate cyclase (GC) in human ciliary body,trabecular meshwork and the Schlemm's canal.Methods Twelve eyes after corneal transplantation were used.Expression of three NOS isoforms (i.e.neuronal NOS (nNOS),inducible NOS (iNOS) and endothelial NOS (eNOS)) and GC were assessed in 10 eyes by immunohistochemical staining using monoclonal or polyclonal antibody of NOS and GC.Ciliary bodies were dissected free and the total proteins were extracted.Western blotting was performed to confirm the protein expression of 3 NOS isoforms and GC.Results Expression of 3 NOS isoforms and GC were observed in the ciliary epithelium,ciliary muscle,trabecular meshwork and the endothelium of the Schlemm's canal.Immunoreactivity of nNOS was detected mainly along the apical cytoplasmic junction of the non-pigmented epithelium (NPE) and pigmented epithelial (PE) cells.Protein expressions of 3 NOS isoforms and GC were confirmed in isolated human ciliary body by Western blotting.Conclusions The expression of NOS isoforms and GC in human ciliary body suggest the possible involvement of NO and cyclic guanosine monophosphate (cyclic GMP,cGMP) signaling pathway in the ciliary body,and may play a role in both processes of aqueous humor formation and drainage.

  9. Adenoma of nonpigmented epithelium in ciliary body:literature review and case report

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Adenomas of the nonpigmented ciliary epithelium (NPCE) are often clinically indistinguishable from amelanotic malignant melanomas of the ciliary body or metastatic carcinomas. This paper reports a case study of a distinctive variant of adenoma of the NPCE, which clinically appears as epiretinal membrane in the macular region. Histopathologic studies have revealed this is an adenoma of the NPCE. Identification of this clinic feature is important because it will miss the diagnosis of the adenoma of the NPCE. In this case study, B-scan ultrasonography as well as computerized tomography (CT) has been used to provide help in diagnosing the ciliary body tumor. Because of their anterior location in the ciliary body, partial lamellar sclerouvectomy is an effective method of treatment.

  10. Roles of paroxetine and corticosterone on adult mammalian ciliary body cell proliferation

    Institute of Scientific and Technical Information of China (English)

    WANG Hua; LAU Benson WM; YAU Suk-yu; LI Suk-yee; LEUNG Nelson; WANG Ning-li; TANG Siu-wa; LEE Tatia MC; SO Kwok-fai

    2010-01-01

    Background The neurogenesis in retina of adult mammals is generally abolished, and this renders the retina lack of regenerative capacity.Despite this, there is a small population of nestin-positive cells in the ciliary epithelium which retains neurogenic potential.The present study aimed at investigating the effect of two drugs, corticosterone and paroxetine, on the cell proliferation of the ciliary body.Methods Adult Sprague-Dawley rats were given vehicle, corticosterone, paroxetine, or both corticosterone and paroxetine treatment for 14 days.Cell proliferation in the ciliary body was quantified using 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry.Co-labelling of BrdU and stem cell marker was used to phenotype the BrdU immunoreactive cells.Results Corticosterone treatment suppressed while paroxetine treatment increased the cell proliferation of the ciliary body.Co-labelling with cell markers revealed that the BrdU positive cells also showed nestin expression but not glial fibrillary acidic protein (GFAP).Conclusions The results illustrate that proliferation of retinal progenitor cells situated in ciliary body are subjected to regulation by selective serotonin reuptake inhibitors (SSRI) and corticosteroid, which is similar to our previous findings in neurogenic regions in central nervous system (CNS).Paroxetine treatment could reverse the suppressive effect of corticosterone on ciliary body cell proliferation.This provides information for future investigation of retinal stem cell biology and potential treatment of retinal degenerative diseases.

  11. Multicenter analysis of body mass index, lung function, and sputum microbiology in primary ciliary dyskinesia

    DEFF Research Database (Denmark)

    Maglione, Marco; Bush, Andrew; Nielsen, Kim G;

    2014-01-01

    BACKGROUND: No studies longitudinally, simultaneously assessed body mass index (BMI) and spirometry in primary ciliary dyskinesia (PCD). METHODS: We determined BMI and spirometry in 158 PCD children and adolescents from London, UK (n = 75), Naples, Italy (n = 23) and Copenhagen, Denmark (n = 60) ...

  12. Defining the proteome of human iris, ciliary body, retinal pigment epithelium, and choroid.

    Science.gov (United States)

    Zhang, Pingbo; Kirby, David; Dufresne, Craig; Chen, Yan; Turner, Randi; Ferri, Sara; Edward, Deepak P; Van Eyk, Jennifer E; Semba, Richard D

    2016-04-01

    The iris is a fine structure that controls the amount of light that enters the eye. The ciliary body controls the shape of the lens and produces aqueous humor. The retinal pigment epithelium and choroid (RPE/choroid) are essential in supporting the retina and absorbing light energy that enters the eye. Proteins were extracted from iris, ciliary body, and RPE/choroid tissues of eyes from five individuals and fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. In iris, ciliary body, and RPE/choroid, we identified 2959, 2867, and 2755 nonredundant proteins with peptide and protein false-positive rates of database of the human iris, ciliary body, and RPE/choroid may serve as a valuable resource for future investigations of the eye in health and disease. The MS proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD001424 and PXD002194. PMID:26834087

  13. The Masquerades of a Childhood Ciliary Body Medulloepithelioma: A Case of Chronic Uveitis, Cataract, and Secondary Glaucoma

    Directory of Open Access Journals (Sweden)

    Jocelyn Chua

    2012-01-01

    Full Text Available Ciliary body medulloepitheliomas in childhood often masquerade other intraocular conditions due to its insidious nature as well as its secondary effects on proximal intraocular tissues in the anterior chamber. We report a case where a ciliary body medulloepithelioma in a two-year-old boy presents with chronic uveitis, cataract, and an uncontrolled secondary glaucoma after an innocuous blunt ocular trauma. The diagnosis was only made after the occurrence of a ciliary body mass. We discuss the clinical features of ciliary body medulloepitheliomas, the implications of a delayed diagnosis and treatment as well as the concern of periorbital tumor seeding with the use of an aqueous shunt implant in this case.

  14. Effect of cAMP on short-circuit current in isolated human ciliary body

    Institute of Scientific and Technical Information of China (English)

    WU Ren-yi; MA Ning; HU Qian-qian

    2013-01-01

    Background Cyclic adenosine monophosphate (cAMP) could activate chloride channels in bovine ciliary body and trigger an increase in the ionic current (short-circuit current,Isc) across the ciliary processes in pigs.The purpose of this study was to investigate how cAMP modulates Isc in isolated human ciliary processes and the possible involvement of chloride transport across the tissue in cAMP-induced Isc change.Methods In an Ussing-type chamber system,the Isc changes induced by the cAMP analogue 8-bromo-cAMP and an adenylyl cyclase activator forskolin in isolated human ciliary processes were assessed.The involvement of Cl-component in the bath solution was investigated.The effect of Cl-channel (10 μmol/L niflumic acid and 1 mmol/L 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS)),K+ channel (10 mmol/L tetraethylammonium chloride (TEA)),or Na+ channel blockers (1 mmol/L amiloride) on 8-bromo-cAMP-induced Isc change was also studied.Results Dose-dependently,8-bromo-cAMP (10 nmol/L-30 μmol/L) or forskolin (10 nmol/L-3 μmol/L) increased Isc across the ciliary processes with an increase in negative potential difference on the non-pigmented epithelium (NPE) side of the tissue.Isc increase induced by 8-bromo-cAMP was more pronounced when the drug was applied on the NPE side than on the pigmented epithelium side.When the tissue was bathed in low Cl-solutions,the Isc increase was significantly inhibited.Finally,niflumic acid and DIDS,but not TEA or amiloride,significantly prevented the Isc increase induced by 8-bromo-cAMP.Conclusions cAMP stimulates stroma-to-aqueous anionic transport in isolated human ciliary processes.Chloride is likely to be among the ions,the transportation of which across the tissue is triggered by cAMP,suggesting the potential role of cAMP in the process of aqueous humor formation in human eyes.

  15. Development of the ciliary body: morphological changes in the distal portion of the optic cup in the human.

    Science.gov (United States)

    Peces-Peña, M D; de la Cuadra-Blanco, C; Vicente, A; Mérida-Velasco, J R

    2013-01-01

    This study seeks to determine the main events that occur in the development of the ciliary body (CB) in the 5-14th week of development. The CB develops from the distal portion of the optic cup (OC) and the neighboring mesenchyme. During the 5th week of development, 4 zones were observed in the distal portion of the OC: in zone 1, the epithelia of the outer and inner layers of the OC came into contact. This contact coincided with the appearance of mainly apical granule pigments. This zone corresponded to the anlage of the epithelial layers of the CB. In zone 2, the cells surrounded the marginal sinus and contained scarce pigment granules and nuclei in the basal position. This zone corresponded to the anlage of the iris. Zone 3 was triangular in shape and its vertex ran towards the marginal sinus and corresponded to common cell progenitors. Zone 4 corresponded to the retinal pigment epithelium anlage and the neural retina anlage. We determined the onset of the stroma and the ciliary muscle anlage at the end of the 7th week. In the 13-14th week, we observed the anlage of the orbicularis ciliaris (pars plana of the CB) and corona ciliaris (pars plicata of the CB), in addition to the anlage of the ciliary muscle. Our study, therefore, establishes a precise timetable of the development of the CB.

  16. Intracranial meningeal melanocytoma associated with nevus of Ota.

    Science.gov (United States)

    Pan, Hao; Wang, Handong; Fan, Youwu

    2011-11-01

    We report a rare intracranial meningeal melanocytoma associated with the nevus of Ota. The patient was 36-year-old man with a 2-week history of headache and difficulty in opening his right eye. Physical examination showed a black nevus scattered on the right-hand side of the face, right eyelid ptosis and papilledema. CT scans and MRI showed a tumor in the region of the right cavernous sinus. The tumor was subtotally resected. Histological examination confirmed the diagnosis of melanocytoma.

  17. Recessive HYDIN mutations cause primary ciliary dyskinesia without randomization of left-right body asymmetry.

    Science.gov (United States)

    Olbrich, Heike; Schmidts, Miriam; Werner, Claudius; Onoufriadis, Alexandros; Loges, Niki T; Raidt, Johanna; Banki, Nora Fanni; Shoemark, Amelia; Burgoyne, Tom; Al Turki, Saeed; Hurles, Matthew E; Köhler, Gabriele; Schroeder, Josef; Nürnberg, Gudrun; Nürnberg, Peter; Chung, Eddie M K; Reinhardt, Richard; Marthin, June K; Nielsen, Kim G; Mitchison, Hannah M; Omran, Heymut

    2012-10-01

    Primary ciliary dyskinesia (PCD) is a genetically heterogeneous recessive disorder characterized by defective cilia and flagella motility. Chronic destructive-airway disease is caused by abnormal respiratory-tract mucociliary clearance. Abnormal propulsion of sperm flagella contributes to male infertility. Genetic defects in most individuals affected by PCD cause randomization of left-right body asymmetry; approximately half show situs inversus or situs ambiguous. Almost 70 years after the hy3 mouse possessing Hydin mutations was described as a recessive hydrocephalus model, we report HYDIN mutations in PCD-affected persons without hydrocephalus. By homozygosity mapping, we identified a PCD-associated locus, chromosomal region 16q21-q23, which contains HYDIN. However, a nearly identical 360 kb paralogous segment (HYDIN2) in chromosomal region 1q21.1 complicated mutational analysis. In three affected German siblings linked to HYDIN, we identified homozygous c.3985G>T mutations that affect an evolutionary conserved splice acceptor site and that subsequently cause aberrantly spliced transcripts predicting premature protein termination in respiratory cells. Parallel whole-exome sequencing identified a homozygous nonsense HYDIN mutation, c.922A>T (p.Lys307(∗)), in six individuals from three Faroe Island PCD-affected families that all carried an 8.8 Mb shared haplotype across HYDIN, indicating an ancestral founder mutation in this isolated population. We demonstrate by electron microscopy tomography that, consistent with the effects of loss-of-function mutations, HYDIN mutant respiratory cilia lack the C2b projection of the central pair (CP) apparatus; similar findings were reported in Hydin-deficient Chlamydomonas and mice. High-speed videomicroscopy demonstrated markedly reduced beating amplitudes of respiratory cilia and stiff sperm flagella. Like the hy3 mouse model, all nine PCD-affected persons had normal body composition because nodal cilia function is

  18. Gene expression and functional annotation of the human ciliary body epithelia.

    Directory of Open Access Journals (Sweden)

    Sarah F Janssen

    Full Text Available PURPOSE: The ciliary body (CB of the human eye consists of the non-pigmented (NPE and pigmented (PE neuro-epithelia. We investigated the gene expression of NPE and PE, to shed light on the molecular mechanisms underlying the most important functions of the CB. We also developed molecular signatures for the NPE and PE and studied possible new clues for glaucoma. METHODS: We isolated NPE and PE cells from seven healthy human donor eyes using laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44×k Agilent microarrays. For microarray conformations, we used a literature study, RT-PCRs, and immunohistochemical stainings. We analyzed the gene expression data with R and with the knowledge database Ingenuity. RESULTS: The gene expression profiles and functional annotations of the NPE and PE were highly similar. We found that the most important functionalities of the NPE and PE were related to developmental processes, neural nature of the tissue, endocrine and metabolic signaling, and immunological functions. In total 1576 genes differed statistically significantly between NPE and PE. From these genes, at least 3 were cell-specific for the NPE and 143 for the PE. Finally, we observed high expression in the (NPE of 35 genes previously implicated in molecular mechanisms related to glaucoma. CONCLUSION: Our gene expression analysis suggested that the NPE and PE of the CB were quite similar. Nonetheless, cell-type specific differences were found. The molecular machineries of the human NPE and PE are involved in a range of neuro-endocrinological, developmental and immunological functions, and perhaps glaucoma.

  19. Characteristics of optic disc melanocytomas presenting with visual dysfunction

    Directory of Open Access Journals (Sweden)

    Al-Rashaed Saba

    2010-01-01

    Full Text Available Objective and Design: A retrospective review study was designed to describe five cases of optic disc melanocytomas with tumor-related visual impairment. Participants: Five patients with optic disc melanocytoma presented with visual complaints to a tertiary eye hospital in Saudi Arabia. Materials and Methods: Demographic and clinical data were analyzed, including the results of ocular examination, lesion laterality, best-corrected Snellen visual acuity, pupillary reflex, visual field testing, color fundus photography, fundus fluorescein angiography, and ophthalmic ultrasound. Results: Visual dysfunction secondary to optic disc melanocytoma was identified. Case 1 had macular star edema with mild tumor enlargement, Case 2 had optic atrophy, Case 3 had juxtapapillary choroidal neovascular membrane with macular involvement, Case 4 had optic disc swelling with an enlarged blind spot, and Case 5 had a large altitudinal visual field defect. Conclusion: Although melanocytomas of the optic disc tend to have a benign behavior with slow evolution and stable vision, they may adversely affect visual function through a variety of mechanisms.

  20. Adenoma of the Nonpigmented Ciliary Body and Iris Epithelium in Mexican Mestizo Patients

    Science.gov (United States)

    Serna-Ojeda, Juan Carlos; Ariza-Camacho, Enrique; Collado-Solórzano, Alberto; Flores-Sánchez, Blanca C.; Rodríguez-Reyes, Abelardo A.; Fulda-Graue, Emiliano

    2015-01-01

    The adenoma of the nonpigmented ciliary epithelium is a benign rare tumor, which may present with different clinical characteristics and requires resection along with histopathologic analysis and the identification of specific immunohistochemical markers for an accurate diagnosis. Here, we report a case series of 4 patients in a Mexican mestizo population with this diagnosis, their clinical features, the ultrasound imaging characteristics and the histopathological and immunohistochemical findings. PMID:27171918

  1. Effect of primary iris and ciliary body cyst on anterior chamber angle in patients with shallow anterior chamber

    Institute of Scientific and Technical Information of China (English)

    Bing-hong WANG; Yu-feng YAO

    2012-01-01

    Objective:To evaluate the prevalence of primary iris and/or ciliary body cysts in eyes with shallow anterior chamber and their effect on the narrowing of the anterior chamber angle.Methods:Among the general physical check-up population,subjects with shallow anterior chambers,as judged by van Herick technique,were recruited for further investigation.Ultrasound biomicroscope (UBM) was used to detect and measure the cysts located in the iris and/or ciliary body,the anterior chamber depth (ACD),the angle opening distance at 500 μm (AOD500),and the trabecular-iris angle (TIA).A-scan ultrasonography was used to measure the ocular biometry,including lens thickness,axial length,lens/axial length factor (LAF),and relative lens position (RLP).The effect of the cyst on narrowing the corresponding anterior chamber angle and the entire angle was evaluated by the UBM images,ocular biometry,and gonioscopic grading.The eye with unilateral cyst was compared with the eye without the cyst for further analysis.Results:Among the 727 subjects with shallow anterior chamber,primary iris and ciliary body cysts were detected in 250 (34.4%) patients; among them 96 (38.4%) patients showed unilateral single cyst,21 (8.4%) patients had unilateral double cysts,and 42 (16.8%) patients manifested unilateral multiple and multi-quadrants cysts.Plateau iris configuration was found in 140 of 361 (38.8%) eyes with cysts.The mean size of total cysts was (0.6547±0.2319) mm.In evaluation of the effect of the cyst size and location on narrowing the corresponding angle to their position,the proportion of the cysts causing corresponding angle narrowing or closure among the cysts larger than 0.8 mm (113/121,93.4%) was found to be significantly higher than that of the cysts smaller than 0.8 mm (373/801,46.6%),and a significant higher proportion was also found in the cysts located at iridociliary sulcus (354/437,81.0%) than in that at the pars plicata (131/484,27.1%).In evaluating the effect

  2. Unusual Tonsillar Herniation in Meningeal Melanocytoma: A Case Report

    Directory of Open Access Journals (Sweden)

    Kaveh Samimi

    2012-01-01

    Full Text Available Meningeal melanocytoma is a primary melanocytic neoplasm with certain MR and immunohistochemical characteristics worthy to note. In a 38-year-old man with a complaint of headache for a couple of years and recently added nausea, vomiting, diplopia, progressive visual blurring and hearing loss, magnetic resonance imaging (MRI was remarkable for T1 shortening of leptomeninges and certain nodules in precontrast study. Subsequent contrast-enhanced MR imaging of the brain and spine revealed enhancement in the basal cisterns extending throughout the spinal canal. Contrast-enhanced MRI revealed diffuse enhancement in the basal cisterns extending throughout the spinal canal. Immunohistochemical analysis on one of the intraspinal nodules proposed leptomeningeal melanocytoma. The characteristic shortening of T1 and T2 relaxation times in MRI as a result of the paramagnetic stable free radicals that exist within melanin, often suggests a diagnosis of a melanocytic leptomeningeal process. Moreover, there are unique immunohistochemical characteristics for this varied lesions.In appropriate clinical settings, certain radiologic findings, especially both T1 and T2 shortening in nodular CNS lesions should propose meningeal melanocytoma.

  3. Megalin–deficiency causes high myopia, retinal pigment epithelium-macromelanosomes and abnormal development of the ciliary body in mice

    DEFF Research Database (Denmark)

    Storm, Tina; Heegaard, Steffen; Christensen, Erik I;

    2014-01-01

    In man, mutations of the megalin-encoding gene causes the rare Donnai-Barrow/Facio-Oculo-Acoustico-Renal Syndrome, which is partially characterized by high-grade myopia. Previous studies of renal megalin function have established that megalin is crucial for conservation of renal filtered nutrients...... that megalin localizes to vesicular structures in the RPE and NPCBE cells. Histological investigations of ocular mouse tissue also identified a severe myopia phenotype as well as enlarged RPE melanosomes and abnormal ciliary body development in the megalin-deficient mice. In conclusion, the complex ocular...... phenotype observed in the megalin-deficient mice suggests that megalin-mediated developmental abnormalities may contribute to the high myopia phenotype observed in the Donnai-Barrow Syndrome patients and, thus, that megalin harbors important roles in ocular development and physiology. Finally, our data show...

  4. Pathogenesis of Congenital Rubella Virus Infection in Human Fetuses: Viral Infection in the Ciliary Body Could Play an Important Role in Cataractogenesis

    Directory of Open Access Journals (Sweden)

    Thong Van Nguyen

    2015-01-01

    Interpretation: Our study based on the pathological examination demonstrated that the rubella virus infection occurred via systemic organs of human fetuses. This fact was confirmed by immunohistochemistry and direct detection of viral RNA in multiple organs. To the best of our knowledge, this study is the first report demonstrating that the rubella virus infection occurred via systemic organs of the human body. Importantly, virus infection of the ciliary body could play an important role in cataractogenesis.

  5. Surgical treatment of iris and ciliary body melanoma: follow-up of a 25-year series of patients

    DEFF Research Database (Denmark)

    Klauber, Stefan; Jensen, Peter K; Prause, Jan U;

    2010-01-01

    Purpose: To evaluate outcome of surgical resection of iris and irido-ciliary melanomas. Method: Retrospective analysis of all cases treated in Denmark 1975-1999 with clinical follow-up in 2002 and death certificate analysis in 2008. A quality of life questionnaire was completed at follow-up. Resu......Purpose: To evaluate outcome of surgical resection of iris and irido-ciliary melanomas. Method: Retrospective analysis of all cases treated in Denmark 1975-1999 with clinical follow-up in 2002 and death certificate analysis in 2008. A quality of life questionnaire was completed at follow...... had died of melanoma-related causes. Only one patient had a local recurrence, which was successfully treated by cryotherapy. The quality of life-related questions demonstrated that most patients (40) suffered from photophobia, and eight patients had changed their driving habits, not driving at night...

  6. Mechanism of ciliary disassembly.

    Science.gov (United States)

    Liang, Yinwen; Meng, Dan; Zhu, Bing; Pan, Junmin

    2016-05-01

    As motile organelles and sensors, cilia play pivotal roles in cell physiology, development and organ homeostasis. Ciliary defects are associated with a class of cilia-related diseases or developmental disorders, termed ciliopathies. Even though the presence of cilia is required for diverse functions, cilia can be removed through ciliary shortening or resorption that necessitates disassembly of the cilium, which occurs normally during cell cycle progression, cell differentiation and in response to cellular stress. The functional significance of ciliary resorption is highlighted in controlling the G1-S transition during cell cycle progression. Internal or external cues that trigger ciliary resorption initiate signaling cascades that regulate several downstream events including depolymerization of axonemal microtubules, dynamic changes in actin and the ciliary membrane, regulation of intraflagellar transport and posttranslational modifications of ciliary proteins. To ensure ciliary resorption, both the active disassembly of the cilium and the simultaneous inhibition of ciliary assembly must be coordinately regulated. PMID:26869233

  7. Intermediate-grade meningeal melanocytoma associated with nevus of Ota: a case report and review of the literature.

    Science.gov (United States)

    Shin, Donghoon; Sinha, Milind; Kondziolka, Douglas S; Kirkwood, John M; Rao, Uma N M; Tarhini, Ahmad A

    2015-08-01

    Meningeal melanocytomas are rare melanin-producing tumors that are often found to be benign. However, a small subset of these tumors can present as intermediate-grade melanocytomas (IGMs) that have histopathological features that are between those of benign melanocytomas and malignant melanomas. IGMs have the potential to recur and metastasize or progress to a more histologically high grade melanoma. Melanocytomas appear to differ from primary and metastatic melanoma by their prolonged clinical course and they appear to have different driver mutations (i.e. mutation of GNAQ gene). The association of a meningeal melanocytoma with nevus of Ota is extremely rare. To our knowledge, there have been only 10 reported cases of synchronous occurrence and only one of the cases involved an IGM. We report the second case of intermediate-grade meningeal melanocytoma that is associated with congenital nevus of Ota. Histopathological work-up confirmed the intermediate grade of the lesion and a driver GNAQ mutation was identified consistent with previous reports.

  8. Primary Meningeal Melanocytoma in the Left Temporal Lobe Associated with Nevus Ota

    DEFF Research Database (Denmark)

    Samadian, Mohammad; Nejad, Ali Mousavi; Bakhtevari, Mehrdad Hosseinzadeh;

    2015-01-01

    histopathologic grade was benign (WHO grade I), radiotherapy was not advised for the patient and he followed up every 6 months. No tumor was seen on follow-up MRI one year after surgery. CONCLUSION: Presentation of meningeal melanocytoma in the supratentorial compartment is rare, and its combination with nevus...... Ota has been reported in very few cases. Although this lesion is benign, it might behave aggressively. Complete surgical resection of the lesion is the preferred therapeutic option....

  9. Long-term Results of the UCSF-LBNL Randomized Trial: Charged Particle With Helium Ion Versus Iodine-125 Plaque Therapy for Choroidal and Ciliary Body Melanoma

    Energy Technology Data Exchange (ETDEWEB)

    Mishra, Kavita K., E-mail: Kavita.mishra@ucsf.edu [Department of Radiation Oncology, University of California-San Francisco, San Francisco, California (United States); Quivey, Jeanne M.; Daftari, Inder K. [Department of Radiation Oncology, University of California-San Francisco, San Francisco, California (United States); Lawrence Berkeley National Laboratory, Berkeley, California (United States); Weinberg, Vivian [Department of Radiation Oncology, University of California-San Francisco, San Francisco, California (United States); Cole, Tia B. [The Tumori Foundation, San Francisco, California (United States); Patel, Kishan [Department of Radiation Oncology, University of California-San Francisco, San Francisco, California (United States); Castro, Joseph R.; Phillips, Theodore L. [Department of Radiation Oncology, University of California-San Francisco, San Francisco, California (United States); Lawrence Berkeley National Laboratory, Berkeley, California (United States); Char, Devron H. [The Tumori Foundation, San Francisco, California (United States); Department of Ophthalmology, University of California-San Francisco, San Francisco, California (United States); Department of Ophthalmology, Stanford University, Palo Alto, California (United States)

    2015-06-01

    Purpose: Relevant clinical data are needed given the increasing national interest in charged particle radiation therapy (CPT) programs. Here we report long-term outcomes from the only randomized, stratified trial comparing CPT with iodine-125 plaque therapy for choroidal and ciliary body melanoma. Methods and Materials: From 1985 to 1991, 184 patients met eligibility criteria and were randomized to receive particle (86 patients) or plaque therapy (98 patients). Patients were stratified by tumor diameter, thickness, distance to disc/fovea, anterior extension, and visual acuity. Tumors close to the optic disc were included. Local tumor control, as well as eye preservation, metastases due to melanoma, and survival were evaluated. Results: Median follow-up times for particle and plaque arm patients were 14.6 years and 12.3 years, respectively (P=.22), and for those alive at last follow-up, 18.5 and 16.5 years, respectively (P=.81). Local control (LC) for particle versus plaque treatment was 100% versus 84% at 5 years, and 98% versus 79% at 12 years, respectively (log rank: P=.0006). If patients with tumors close to the disc (<2 mm) were excluded, CPT still resulted in significantly improved LC: 100% versus 90% at 5 years and 98% versus 86% at 12 years, respectively (log rank: P=.048). Enucleation rate was lower after CPT: 11% versus 22% at 5 years and 17% versus 37% at 12 years, respectively (log rank: P=.01). Using Cox regression model, likelihood ratio test, treatment was the most important predictor of LC (P=.0002) and eye preservation (P=.01). CPT was a significant predictor of prolonged disease-free survival (log rank: P=.001). Conclusions: Particle therapy resulted in significantly improved local control, eye preservation, and disease-free survival as confirmed by long-term outcomes from the only randomized study available to date comparing radiation modalities in choroidal and ciliary body melanoma.

  10. Intracranial intermediate-grade meningeal melanocytoma with increased cellular proliferative index: an illustrative case associated with a nevus of Ota.

    Science.gov (United States)

    Navas, Marta; Pascual, José M; Fraga, Javier; Pedrosa, Manuel; Shakur, Sophia; Carrasco, Rodrigo; Martínez, Pedro; Manzanares, Rafael; de Sola, Rafael García

    2009-10-01

    Solitary primary melanocytic tumors of the central nervous system (CNS) represent a spectrum of lesions ranging from well-differentiated melanocytoma to melanoma. The association of a meningeal melanocytoma with an ipsilateral nevus of Ota is extremely rare, with only six cases reported in the literature to date. Only a minority of melanocytic tumors correspond to lesions of intermediate-grade malignancy, whose biological behavior and outcome remain undetermined. We report a new case of a 25-year-old man with a giant fronto-temporal intracranial meningeal melanocytoma associated with a congenital nevus of Ota, who suffered an acute neurological deterioration requiring an emergent surgical procedure. Despite total removal of the lesion, the patient did not recover from the operation due to a malignant infarct of the right hemisphere. The lesion showed no histological criteria of malignancy but did exhibit an elevated (8%) MIB-1/Ki-67 cell proliferative index, which suggested an intermediate-grade melanocytoma. In addition, its metabolic profile, determined by in vivo proton magnetic resonance spectroscopy (MRS), was similar to that observed in most high-grade gliomas. To our knowledge, this is the first case of a meningeal melanocytoma of intermediate grade associated with a nevus of Ota reported in the literature. Only two additional intermediate-grade melanocytomas showing an elevated MIB-1/Ki-67 labeling index have been previously reported. The MIB-1/Ki-67 labeling index may have potential prognostic value in helping the clinician to predict an aggressive clinical behavior and/or malignant progression for primary melanocytic neoplasms of the CNS.

  11. Geometry of ciliary dynamics

    Science.gov (United States)

    Peterson, Mark A.

    2009-07-01

    Cilia are motile biological appendages that are driven to bend by internal shear stresses between tubulin filaments. A continuum model of ciliary material is constructed that incorporates the essential ciliary constraints: (i) one-dimensional inextensibility of filaments, (ii) three-dimensional incompressibility, and (iii) shear strain only longitudinally along filaments. It is shown that twist of filaments about each other is not an independent degree of freedom under ciliary constraints. The constraint on twist appears in the equations of motion for cilia as a term not previously recognized. As another application of the same geometrical idea, a general approach to the polymorphism of bacterial flagella is proposed.

  12. A case report on intermediate-grade malignant meningeal melanocytoma with elevated cell proliferation index.

    Science.gov (United States)

    Sun, Jian; Wang, Chengjun; Shu, Qingming; Liu, Jie; Mao, Gengsheng

    2015-01-01

    Meningeal melanocytoma is a kind of extremely rare pigmented tumor of the central nervous system, which often occurs in the groove around the base of the brain and spinal pia mater. Age of onset is 40 to 50 years old, mostly presenting a benign course of disease and the prognosis is good. Case reports of partial invasion or metastasis from lesions are even rarer. This report described a case of 56-year-old meningeal melanoma patient with partial skull and muscle invasion at the lantooccipital transition zone. Intraoperative histological examination showed moderate malignancy and Ki-67 index was 10%. PMID:26617913

  13. The GNAQ in the haystack: intramedullary meningeal melanocytoma of intermediate grade at T9-10 in a 58-year-old woman.

    Science.gov (United States)

    Hoffmann, Michael; Koelsche, Christian; Seiz-Rosenhagen, Marcel; Mai, Sabine; Lohr, Frank; Reuss, David; Wenz, Frederik; Gebhardt, Christoffer; Giordano, Frank A

    2016-07-01

    Meningeal melanocytomas are rare tumors. They are derived from leptomeningeal melanocytes and predominantly occur along the spine and the posterior fossa. Here, the authors report a case of intramedullary melanocytoma of intermediate grade in a 58-year-old female patient who was initially misdiagnosed with malignant melanoma until mutational analyses of a panel of genes associated with melanotic tumors led to reclassification. PMID:26544768

  14. Primary ciliary dyskinesiatwo cases reports

    Directory of Open Access Journals (Sweden)

    Mohammad Sadegh Rezaee1

    2009-01-01

    Full Text Available (Received 22 December, 2009 ; Accepted 10 March, 2010AbstractPrimary ciliary dyskinesia and Kartagener's syndrome are rare genetic disorders. There is a ciliary dysfunction in these disorders that cause recurrent infections in respiratory and sinus tracts associated with dextrocardia, chronic vasomotor rhinitis and dextrocardia. The aim of this paper is to report two rare cases of Primary ciliary dyskinesia, including one case of primary ciliary dyskinesia and Kartagener's syndrome for additional knowledge. J Mazand Univ Med Sci 2009; 19(73: 85-89 (Persian.

  15. Primary Ciliary Dyskinesia.

    Science.gov (United States)

    Knowles, Michael R; Zariwala, Maimoona; Leigh, Margaret

    2016-09-01

    Primary ciliary dyskinesia (PCD) is a recessive genetically heterogeneous disorder of motile cilia with chronic otosinopulmonary disease and organ laterality defects in ∼50% of cases. The prevalence of PCD is difficult to determine. Recent diagnostic advances through measurement of nasal nitric oxide and genetic testing has allowed rigorous diagnoses and determination of a robust clinical phenotype, which includes neonatal respiratory distress, daily nasal congestion, and wet cough starting early in life, along with organ laterality defects. There is early onset of lung disease in PCD with abnormal airflow mechanics and radiographic abnormalities detected in infancy and early childhood. PMID:27514592

  16. Primary ciliary dyskinesia.

    Science.gov (United States)

    Lobo, Jason; Zariwala, Maimoona A; Noone, Peadar G

    2015-04-01

    Primary ciliary dyskinesia (PCD) is an autosomal recessive disorder of cilia structure, function, and biogenesis leading to chronic infections of the respiratory tract, fertility problems, and disorders of organ laterality. The diagnosis can be challenging, using traditional tools such as characteristic clinical features, ciliary function, and ultrastructural defects and newer screening tools such as nasal nitric oxide levels and genetic testing add to the diagnostic algorithm. There are 32 known PCD-causing genes, and in the future, comprehensive genetic testing may screen young infants before developing symptoms, thus improving survival. Therapies include surveillance of pulmonary function and microbiology, in addition to airway clearance, antibiotics, and early referral to bronchiectasis centers. As with cystic fibrosis (CF), standardized care at specialized centers using a multidisciplinary approach likely improves outcomes. In conjunction with the CF foundation, the PCD foundation, with experienced investigators and clinicians, is developing a network of PCD clinical centers to coordinate the effort in North America and Europe. As the network grows, clinical care and knowledge will improve. PMID:25826585

  17. Cistos primários do epitélio pigmentar da íris e corpo ciliar: aspectos de biomicroscopia ultra-sônica Primary cysts of the iris and ciliary body pigment epithelium: ultrasound biomicroscopy features

    Directory of Open Access Journals (Sweden)

    Bernadete Ayres

    2000-10-01

    Full Text Available Objetivo: Descrever as características, incidência e distribuição dos cistos primários de epitélio pigmentar de íris e corpo ciliar ao exame de biomicroscopia ultra-sônica, que devem ser diferenciados de lesões sólidas. Métodos: Foram estudados de modo retrospectivo os prontuários de 73 pacientes, 82 olhos, com diagnóstico ecográfico de cisto primário de íris ou corpo ciliar durante o período de janeiro/97 a dezembro/99. Utilizou-se o biomicroscópio ultra-sônico, aplicando técnicas padronizadas de imersão. Resultados: À biomicroscopia ultra-sônica os cistos caracterizaram-se por apresentarem paredes finas e regulares, e conteúdo anecóico. Quarenta e oito pacientes (65,7% eram do sexo feminino. A maior incidência (28,8% ocorreu para o grupo incluído no intervalo de 20 a 29 anos de idade. Observou-se uma característica distribuição, predominantemente nos quadrantes temporais inferiores. Conclusões: A biomicroscopia ultra-sônica mostrou-se útil no diagnóstico de cistos primários do epitélio pigmentar da íris e do corpo ciliar, auxiliando na diferenciação de patologias tumorais e avaliando possíveis complicações. O conhecimento dos critérios ecográficos e da distribuição epidemiológica facilitam o diagnóstico destas lesões.Purpose: To describe the ultrasound biomicroscopic characteristics, incidence, distribution and location of primary cysts of the iris and ciliary body pigment epithelium, and to differentiate them from solid lesions. Methods: A retrospective study was performed through a review of charts of 73 patients, 82 eyes, with echographic diagnosis of primary cysts of the iris and ciliary body pigment epithelium during a 36-month period (January/97 through December/99. All examinations were performed using an ultrasound biomicroscope applying standard immersion techniques. Results: Ultrasound biomicroscopy revealed typical findings of the primary cysts of the pigment epithelium such as thin

  18. Diversity of vascular permeability in iris and ciliary body after penetrating keratoplasty%穿透角膜移植术后虹膜睫状体血管通透性变化

    Institute of Scientific and Technical Information of China (English)

    刘明娜; 庄宪丽; 高华; 李素霞; 史伟云

    2013-01-01

    Background Allograft rejection is a main cause of failure of penetrating keratoplasty,especially in the patient with high risk of rejection condition.Previous study on allograft rejection mechanism focused on limbal and corneal neovascularization,but these factors did not explain all the phenomena of allograft rejection.Research found that immune cells appeared in iris and ciliary body when rejection occurred,but the relationship between these immune cells and allograft rejection is unclear Objective This study was to evaluate the relationship between diversity of vascular permeability in the iris and ciliary body and allograft rejection after penetrating keratoplasty.Methods Seventy clean eight-week-old BALB/c mice were divided into allogeneic corneal transplantation group (60 mice) and blank control group (10 mice).Allogeneic corneal transplantation was performed with the same age of C57BL/6 mice as donor and BALB/c mice as the recipients.The grafts were examined under the slit lamp microscope and scored based on the criteria of Hegde.The mice were sacrificed and iris and ciliary tissue were obtained 5,10 days and rejection after surgery.Immunohistochemistry and reverse transcription PCR (RT-PCR) was used respectively to detect the expression diversities of occludin,zonula occludens protein-1 (ZO-1),matrix metalloproteinase-9(MMP-9),major histocompatibility complex-Ⅱ (MHC-Ⅱ),and CCR5,CCR7 and their mRNA in iris and ciliary body.Image-J image analysis software was used to calculate the quantity of positive cells on iris wholemount,and absorbance of target genes (A values).The use and care of the experimental animals complied the ARVO Resolution on the Use of Animals in Research.Results The mean survival time of corneal gratts was (17±3) days after operation.The mean score was 0.6 in 5 days and 0.5 in 10 days,and 3.3 in 18 days after operation.Expression of ZO-1 reduced significantly,and that of MMP-9 increased obviously at the time of rejection.MHC

  19. Intraflagellar Transport (IFT) Role in Ciliary Assembly, Resorption and Signalling

    DEFF Research Database (Denmark)

    Pedersen, Lotte B; Rosenbaum, Joel L

    2008-01-01

    Cilia and flagella have attracted tremendous attention in recent years as research demonstrated crucial roles for these organelles in coordinating a number of physiologically and developmentally important signaling pathways, including the platelet-derived growth factor receptor (PDGFR) alpha, Sonic...... is a complex, multistep process that is tightly coordinated with cell cycle progression and differentiation. The ciliary axoneme is extended from a modified centriole, the basal body, which migrates to and docks onto the apical plasma membrane early in ciliogenesis as cells enter growth arrest. The ciliary...

  20. Adult Ocular Toxocariasis Mimicking Ciliary Body Malignancy

    Directory of Open Access Journals (Sweden)

    Ahmad M. Mansour

    2014-01-01

    Full Text Available Purpose. To discuss an unusual presentation of ocular toxocariasis. Methods. Case report. Results. A 40-year-old woman presented with decreased vision in the left eye with a long history of recurrent red eye from uveitis. Eosinophilia and positive ELISA titers for Toxocara canis favored the diagnosis of ocular toxocariasis. Over 3 months, an anterior scleral mass had a rapid growth raising the possibility of medulloepithelioma, which rarely can mimic uveitic syndromes. Surgical plan changed from local excision to enucleation. Histopathology demonstrated a large homogeneous mass of chronic inflammatory cells with inflammation of the overlying thinned out sclera, medial rectus insertion, and limbal cornea. The triad of peripheral granuloma, eosinophilia, and positive blood serology established the diagnosis of ocular toxocariasis. Conclusions. Ocular toxocariasis can mimic ocular malignancy such as medulloepithelioma in adults and rarely presents as an anterior scleral mass.

  1. Ciliary kinematics of Chlamydomonas reinhardtii in Complex Fluids: Role of viscosity

    Science.gov (United States)

    Gopinath, Arvind; Qin, Boyang; Arratia, Paulo

    2014-11-01

    The motility behavior of microorganisms can be significantly affected by the rheology of their fluidic environment. Guided by our experiments on the swimming gait of Chlamydomonas reinhardtii in viscoelastic fluids, we focus on ciliary waveforms in Newtonian fluids and systematically study the effect of increasing viscosity. We find that the beat frequency as well as the wave speed are both strongly influenced by fluid viscosity. Interestingly, ciliary waveforms at low viscosity show a larger influence of the cell body than waveforms at higher viscosity. We use slender body theory and principal component analysis to elucidate the role of fluid viscosity in regulating the kinematics of the swimming process.

  2. Bardet–Biedl syndrome proteins 1 and 3 regulate the ciliary trafficking of polycystic kidney disease 1 protein

    OpenAIRE

    Su, Xuefeng; Driscoll, Kaitlin; Yao, Gang; Raed, Anas; Wu, Maoqing; Beales, Philip L.; Zhou, Jing

    2014-01-01

    Bardet–Biedl syndrome (BBS) and autosomal dominant polycystic kidney disease (ADPKD) are two genetically distinct ciliopathies but share common phenotypes such as renal cysts. Seven BBS proteins form a complex called the BBSome which is localized at the basal body or ciliary axoneme and regulates the ciliary entry or flagellar exit of several signaling molecules. Here, we demonstrate that, unlike the seven-span somatostatin receptor 3 or the leptin receptor that interacts with all subunits of...

  3. Analysis of Axonemal Assembly During Ciliary Regeneration in Chlamydomonas.

    Science.gov (United States)

    Hunter, Emily L; Sale, Winfield S; Alford, Lea M

    2016-01-01

    Chlamydomonas reinhardtii is an outstanding model genetic organism for study of assembly of cilia. Here, methods are described for synchronization of ciliary regeneration in Chlamydomonas to analyze the sequence in which ciliary proteins assemble. In addition, the methods described allow analysis of the mechanisms involved in regulation of ciliary length, the proteins required for ciliary assembly, and the temporal expression of genes encoding ciliary proteins. Ultimately, these methods can contribute to discovery of conserved genes that when defective lead to abnormal ciliary assembly and human disease. PMID:27514926

  4. Beyond the mucus escalator: Complex ciliary hydrodynamics in disease and function

    Science.gov (United States)

    Nawroth, Janna; Guo, Hanliang; John, Dabiri; Kanso, Eva; McFall-Ngai, Margaret

    2015-11-01

    Cilia are microscopic, hair-like structures lining external and internal body surfaces where they interact with fluids. The main function of motile cilia is often described as that of a ``mucus escalator'', i.e., a homogeneous ciliary carpet moving along layer of mucus along the surface to transport food, germ cells, debris, or pathogens. Accordingly, the performance of ciliary systems is usually measured in terms of a single metric, transport velocity, or its presumed proxy, ciliary beat frequency. We challenge this simple view through the observation that both healthy and diseased biological systems exhibit a variety of cilia morphologies, beat patterns, and arrangements, resulting in complex flow patterns and transport phenomena that cannot be reduced to a single parameter. Here we present two case studies. In one system, the ciliated surface creates two distinct flow regimes for first trapping and then sheltering potential symbiont bacteria for further biochemical screening. In the other system, chronic disease induces a misalignment of ciliary beat, leading to a pathological transition from uniform mucus transport to a pattern of stagnation and circulation. These studies suggest that (a), we need to develop a wider range of metrics for describing ciliary transport in biological and clinical contexts, and (b), engineered ciliated systems exploiting a variety of design parameters could provide novel ways of manipulating fluids at the microscale.

  5. Primary ciliary dyskinesia: clinical and genetic aspects

    Directory of Open Access Journals (Sweden)

    E. D’Auria

    2012-06-01

    Full Text Available Primary ciliary dyskinesia (PCD is a rare, genetically heterogeneous disease, characterized by ciliary disfunction and impaired mucociliary clearance, resulting in a range of clinical manifestations such as chronic bronchitis, bronchiectasis, chronic rhino-sinusitis, chronic otitis media, situs viscerum inversus in almost 40-50% of cases and male infertility. The triad situs viscerum inversus, bronchiectasis and sinusitis is known as Kartagener syndrome. Up to now little is known about genetic, diagnostic and therapeutic aspects of primary motile ciliary diseases in children: for this reason, diagnosis is generally delayed and almost all treatments for PCD are not based on randomized studies but extrapolated from cystic fibrosis guidelines. The aim of this review is to propose to pediatricians a summary of current clinical and diagnostic evidence to obtain better knoledwge of this condition. The earlier diagnosis and the right treatment are both crucial to improve the prognosis of PCD.

  6. Voltage-gated potassium channel Kvl.3 in rabbit ciliary epithelium regulates the membrane potential via coupling intracellular calcium

    Institute of Scientific and Technical Information of China (English)

    LI Yan-feng; ZHUO Ye-hong; BI Wei-na; BAI Yu-jing; LI Yan-na; WANG Zhi-jian

    2008-01-01

    Background The cell layer of the ciliary epithelium is responsible for aqueous humor secretion and maintenance.Ion channels play an important role in these processes.The main aim of this study was to determine whether the well-characterized members of the Kvl family (Kv1.3) contribute to the Kv currents in ciliary epithelium.Methods New Zealand White rabbits were maintained in a 12 hours light/dark cycle.Ciliary epithelium samples were isolated from the rabbits.We used Western blotting and immunocytochemistry to identify the expression and location of a voltage-gated potassium channel Kvl.3 in ciliary body epithelium.Membrane potential change after adding of Kv1.3 inhibitor margatoxin (MgTX) was observed with a fluorescence method.Results Western blotting and immunocytochemical studies showed that the Kv1.3 protein expressed in pigment ciliary epithelium and nonpigment ciliary epithelium,however it seemed to express more in the apical membrane of the nonpigmented epithelial cells.One nmol/L margatoxin,a specific inhibitor of Kv1.3 channels caused depolarization of the cultured nonpigmented epithelium (NPE) membrane potential.The cytosotic calcium increased after NPE cell depolarization,this increase of cytosolic calcium was partially blocked by 12.5 μmol/L dantrolene and 10 μmol/L nifedipine.These observations suggest that Kv1.3 channels modulate ciliary epithelium potential and effect calcium dependent mechanisms.Conclusion Kv1.3 channels contribute to K+ efflux at the membrane of rabbit ciliary epithelium.

  7. A safe method of ciliary sulcus fixation of foldable intraocular lens using a ciliary sulcus guide.

    Science.gov (United States)

    Can, Ertuğrul; Gül, Adem; Birinci, Hakkı

    2016-08-01

    To describe a novel technique for implantation of intraocular lens in the absence of capsular support using a ciliary sulcus guide. Based on the anatomic knowledge of the ciliary sulcus and the sclera, a new instrument was developed to pierce the needle safely through the ciliary sulcus and sclera. While the foldable lens is stored inside the cartridge, the leading haptic is sutured with a cow-hitch knot. The needle is then inserted into the ciliary sulcus guide. The tip of the guide is inserted from the corneal incision and proceeded under the iris to touch and fit the ciliary sulcus. The needle is pushed from back side. The needle comes out at precise point at the sclera. Implantation of the lens was performed through a 2.8 mm clear cornea incision using the injector. The trailing haptic is tied after implantation, and then the same procedure is performed at the opposite side. We performed this technique to 15 aphakic eyes without sufficient capsular support. There was no bleeding or other intraoperative complication. All the points coming out the sclera were between 2 and 2.5 mm from the limbus. The ab interno technique for scleral fixation of IOL is quicker, easier and less traumatic then ab externo techniques. A new ciliary sulcus guide which is usable with both straight and curved needles eliminates the blind maneuvers of ab interno technique and makes this technique more safe and precise.

  8. Photoreceptor Sensory Cilium: Traversing the Ciliary Gate

    Directory of Open Access Journals (Sweden)

    Hemant Khanna

    2015-10-01

    Full Text Available Cilia are antenna-like extensions of the plasma membrane found in nearly all cell types. In the retina of the eye, photoreceptors develop unique sensory cilia. Not much was known about the mechanisms underlying the formation and function of photoreceptor cilia, largely because of technical limitations and the specific structural and functional modifications that cannot be modeled in vitro. With recent advances in microscopy techniques and molecular and biochemical approaches, we are now beginning to understand the molecular basis of photoreceptor ciliary architecture, ciliary function and its involvement in human diseases. Here, I will discuss the studies that have revealed new knowledge of how photoreceptor cilia regulate their identity and function while coping with high metabolic and trafficking demands associated with processing light signal.

  9. Evidence for two extremes of ciliary motor response in a single swimming microorganism.

    Science.gov (United States)

    Jung, Ilyong; Powers, Thomas R; Valles, James M

    2014-01-01

    Because arrays of motile cilia drive fluids for a range of processes, the versatile mechano-chemical mechanism coordinating them has been under scrutiny. The protist Paramecium presents opportunities to compare how groups of cilia perform two distinct functions, swimming propulsion and nutrient uptake. We present how the body cilia responsible for propulsion and the oral-groove cilia responsible for nutrient uptake respond to changes in their mechanical environment accomplished by varying the fluid viscosity over a factor of 7. Analysis with a phenomenological model of trajectories of swimmers made neutrally buoyant with magnetic forces combined with high-speed imaging of ciliary beating reveal that the body cilia exert a nearly constant propulsive force primarily by reducing their beat frequency as viscosity increases. By contrast, the oral-groove cilia beat at a nearly constant frequency. The existence of two extremes of motor response in a unicellular organism prompts unique investigations of factors controlling ciliary beating. PMID:24411242

  10. Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila

    OpenAIRE

    Chen, Jieyan V.; Kao, Ling-Rong; Jana, Swadhin C.; Sivan-Loukianova, Elena; Mendonça, Susana; Cabrera, Oscar A.; Singh, Priyanka; Cabernard, Clemens; Eberl, Daniel F.; Bettencourt-Dias, Monica; Timothy L. Megraw

    2015-01-01

    Cilia are essential for cell signaling and sensory perception. In many cell types, a cytoskeletal structure called the ciliary rootlet links the cilium to the cell body. Previous studies indicated that rootlets support the long-term stability of some cilia. Here we report that Drosophila melanogaster Rootletin (Root), the sole orthologue of the mammalian paralogs Rootletin and C-Nap1, assembles into rootlets of diverse lengths among sensory neuron subtypes. Root mutant neurons lack rootlets a...

  11. Gene expression studies in cells from primary ciliary dyskinesia patients identify 208 potential ciliary genes

    NARCIS (Netherlands)

    Geremek, Maciej; Bruinenberg, Marcel; Zietkiewicz, Ewa; Pogorzelski, Andrzej; Wijmenga, Cisca; Witt, Michal

    2011-01-01

    Cilia are small cellular projections that either act as sensors (primary cilia) or propel fluid over the epithelia of various organs (motile cilia). The organellum has gained much attention lately because of its involvement in a group of human diseases called ciliopathies. Primary ciliary dyskinesia

  12. Ciliary disorder of the skeleton.

    Science.gov (United States)

    Huber, Celine; Cormier-Daire, Valerie

    2012-08-15

    In the last 10 years, the primary cilia machinery has been implicated in more than a dozen disorders united as ciliopathies, including skeletal dysplasias, such as Jeune syndrome and short rib-polydactyly type III. Indeed, primary cilia play a vital role in transduction of signals in the hedgehog pathway that is especially important in skeletal development. In this review, we focus on skeletal conditions belonging to the ciliopathy group: the short rib-polydactyly group (SRPs) that includes Verma-Naumoff syndrome (SRP type III), Majewski syndrome (SRP type II), Jeune syndrome (ATD), as well as Ellis-van Creveld syndrome (EVC), the Sensenbrenner syndrome, and, finally, Weyers acrofacial dysostosis. Today, 10 different genes have been identified as responsible for seven "skeletal" ciliopathies. Mutations have been identified in dynein motor (DYNC2H1), in intraflagellar transport (IFT) complexes (IFT80, IFT122, IFT43, WDR35, WDR19, and TTC21B) as well as in genes responsible for the basal body (NEK1, EVC, and EVC2). The wide clinical variability observed for an individual ciliopathy gene supports the development of exome strategy specifically dedicated to cilia genes to identify mutations in this particularly heterogeneous group of disorders.

  13. Biomicroscopia ultra-sônica (UBM no planejamento cirúrgico e acompanhamento pós-operatório de melanomas malignos de íris e corpo ciliar Ultrasound biomicroscopy (UBM in the surgical planning and postoperative follow-up of iris and ciliary body melanomas

    Directory of Open Access Journals (Sweden)

    Zélia Maria da Silva Corrêa

    2003-08-01

    Full Text Available OBJETIVO: Estudar os achados da biomicroscopia ultra-sônica (UBM em melanomas malignos primários da íris e corpo ciliar antes e depois da resecção cirúrgica e correlacionar a extensão do tumor (revelado pela biomicroscopia ultra-sônica aos achados histopatológicos. MÉTODOS: Estudo prospectivo de 5 pacientes com diagnóstico clínico de tumor de íris e/ou corpo ciliar. Estes pacientes foram avaliados pela biomicroscopia ultra-sônica antes e após iridociclectomia ou iridectomia. Os espécimens foram enviados para estudo histopatológico. Os pacientes foram acompanhados por período mínimo de 6 meses quando foi realizada nova biomicroscopia ultra-sônica. RESULTADOS: Neste grupo de 5 pacientes, 3 eram do sexo feminino e 2, do masculino, variação de idade de 32 e 64 anos. Todos os casos eram unilaterais. Quatro pacientes apresentaram massa iridociliar e um apresentou lesão limitada à íris. Em todos os casos a biomicroscopia ultra-sônica auxiliou o planejamento cirúrgico para a ressecção tumoral com margens livres. A biomicroscopia ultra-sônica no pós-operatório revelou ausência de crescimento tumoral em todos os casos, sendo que 2 pacientes apresentaram vítreo na incisão cirúrgica, 1 deles com tração vítrea leve, mas sem sinais de descolamento de retina. CONCLUSÃO: A biomicroscopia ultra-sônica pareceu ser método auxiliar confiável para diagnosticar tumores de íris e corpo ciliar, planejar o tratamento cirúrgico e o acompanhamento pós-operatório.PURPOSE: To study pre-operatively and post-operatively ultrasound biomicroscopy (UBM findings of iris and ciliary body melanomas and correlate tumor extension (revealed by ultrasound biomicroscopy with histopathology findings. METHODS: Prospective study of 5 patients with a clinical diagnosis of iris and ciliary body tumors. These patients were evaluated by ultrasound biomicroscopy before and after treatment with iridectomy or iridociclectomy. All specimens were sent

  14. Artificial ciliary bundles with nano fiber tip links

    CERN Document Server

    Asadnia, Mohsen; Miao, Jianmin; Triantafyllou, Michael

    2015-01-01

    Mechanosensory ciliary bundles in fishes are the inspiration for carefully engineered artificial flow sensors. We report the development of a new class of ultrasensitive MEMS flow sensors that mimic the intricate morphology of the ciliary bundles, including the stereocilia, tip links, and the cupula, and thereby achieve threshold detection limits that match the biological example. An artificial ciliary bundle is achieved by fabricating closely-spaced arrays of polymer micro-pillars with gradiating heights. Tip links that form the fundamental sensing elements are realized through electrospinning aligned PVDF piezoelectric nano-fibers that link the distal tips of the polymer cilia. An optimized synthesis of hyaluronic acid-methacrylic anhydride hydrogel that results in properties close to the biological cupula, together with drop-casting method are used to form the artificial cupula that encapsulates the ciliary bundle. In testing, fluid drag force causes the ciliary bundle to slide, stretching the flexible nan...

  15. Expressions of 11β-hydroxysteroid dehydrogenase type 1 and steroids receptors in ciliary body with glucocorticoid-induced ocular hypertension rabbit model%11β-HSD1及皮质类固醇受体在兔糖皮质激素性高眼压模型睫状体组织中的表达

    Institute of Scientific and Technical Information of China (English)

    刘溢; 张玉杰; 夏丹; 姚志峰; 袁志兰

    2014-01-01

    Background Long-term administration of glucocorticoid drugs induces ocular hypertension in susceptible individuals probably.It has been verified that 1 1β-hydroxysteroid dehydrogenase type 1 (11β-HSD1),glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) can affect the generating of aqueous humor,but how they play the role in glucocorticoid-induced ocular hypertension is unclear.Objective This study was to investigate the relationship of expressions of 11β-HSD1 and steroids receptors in ciliary body and steroid-induced ocular hypertension.Methods Thirteen 12-16 week-old New Zealand albino rabbits were randomized to control group (5 rabbits) and experimental group (8 rabbits).Steroid-induced glaucoma models were induced by administration of subconjunctival injection of 5 mg dexamethasone solution(1 ml) and 0.5% dexamethasone eye drops on alternate days in the left eyes for consecutive two months in the experimental group,and the equal volume of sterile normal saline solution was used in the same way in the control group.The successful criteria of model eyes was defined as rising of intraocular pressure (IOP) to ≥ 18 mmHg for over one week.Then,the animals were sacrificed by excessive anesthesia and the ciliary tissues were isolated for the assay of expressions of 1 1β-HSD1 protein by immunochemistry,and the expressions of 11β-HSD1 mRNA,GR mRNA and MR mRNA in ciliary body were semi-quantitatively detected by reverse transcription-PCR (RT-PCR).The experimental results were compared between the two groups.Results The IOP was normal in the first two weeks after administration of drugs,and no significant difference was found in IOP between the first week and the second week in the experimental group (q =0.469,P >0.05).From 3 through 5 weeks after injection,the IOP was gradually elevated,with the highest value of (18.87±0.77) mmHg in the fifth week.Significant differences were seen between the two groups at mentioned-above time points (q =10

  16. Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function.

    Science.gov (United States)

    Lee, Robert J; Workman, Alan D; Carey, Ryan M; Chen, Bei; Rosen, Phillip L; Doghramji, Laurel; Adappa, Nithin D; Palmer, James N; Kennedy, David W; Cohen, Noam A

    2016-01-01

    Aflatoxins are mycotoxins secreted by Aspergillus flavus, which can colonize the respiratory tract and cause fungal rhinosinusitis or bronchopulmonary aspergillosis. A. flavus is the second leading cause of invasive aspergillosis worldwide. Because many respiratory pathogens secrete toxins to impair mucociliary immunity, we examined the effects of acute exposure to aflatoxins on airway cell physiology. Using air-liquid interface cultures of primary human sinonasal and bronchial cells, we imaged ciliary beat frequency (CBF), intracellular calcium, and nitric oxide (NO). Exposure to aflatoxins (0.1 to 10 μM; 5 to 10 minutes) reduced baseline (~6-12%) and agonist-stimulated CBF. Conditioned media (CM) from A. fumigatus, A. niger, and A. flavus cultures also reduced CBF by ~10% after 60 min exposure, but effects were blocked by an anti-aflatoxin antibody only with A. flavus CM. CBF reduction required protein kinase C but was not associated with changes in calcium or NO. However, AFB2 reduced NO production by ~50% during stimulation of the ciliary-localized T2R38 receptor. Using a fluorescent reporter construct expressed in A549 cells, we directly observed activation of PKC activity by AFB2. Aflatoxins secreted by respiratory A. flavus may impair motile and chemosensory functions of airway cilia, contributing to pathogenesis of fungal airway diseases. PMID:27623953

  17. Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function

    Science.gov (United States)

    Lee, Robert J.; Workman, Alan D.; Carey, Ryan M.; Chen, Bei; Rosen, Phillip L.; Doghramji, Laurel; Adappa, Nithin D.; Palmer, James N.; Kennedy, David W.; Cohen, Noam A.

    2016-01-01

    Aflatoxins are mycotoxins secreted by Aspergillus flavus, which can colonize the respiratory tract and cause fungal rhinosinusitis or bronchopulmonary aspergillosis. A. flavus is the second leading cause of invasive aspergillosis worldwide. Because many respiratory pathogens secrete toxins to impair mucociliary immunity, we examined the effects of acute exposure to aflatoxins on airway cell physiology. Using air-liquid interface cultures of primary human sinonasal and bronchial cells, we imaged ciliary beat frequency (CBF), intracellular calcium, and nitric oxide (NO). Exposure to aflatoxins (0.1 to 10 μM; 5 to 10 minutes) reduced baseline (~6–12%) and agonist-stimulated CBF. Conditioned media (CM) from A. fumigatus, A. niger, and A. flavus cultures also reduced CBF by ~10% after 60 min exposure, but effects were blocked by an anti-aflatoxin antibody only with A. flavus CM. CBF reduction required protein kinase C but was not associated with changes in calcium or NO. However, AFB2 reduced NO production by ~50% during stimulation of the ciliary-localized T2R38 receptor. Using a fluorescent reporter construct expressed in A549 cells, we directly observed activation of PKC activity by AFB2. Aflatoxins secreted by respiratory A. flavus may impair motile and chemosensory functions of airway cilia, contributing to pathogenesis of fungal airway diseases. PMID:27623953

  18. Ciliary abnormalities in senescent human fibroblasts impair proliferative capacity.

    Science.gov (United States)

    Breslin, Loretta; Prosser, Suzanna L; Cuffe, Sandra; Morrison, Ciaran G

    2014-01-01

    Somatic cells senesce in culture after a finite number of divisions indefinitely arresting their proliferation. DNA damage and senescence increase the cellular number of centrosomes, the 2 microtubule organizing centers that ensure bipolar mitotic spindles. Centrosomes also provide the basal body from which primary cilia extend to sense and transduce various extracellular signals, notably Hedgehog. Primary cilium formation is facilitated by cellular quiescence a temporary cell cycle exit, but the impact of senescence on cilia is unknown. We found that senescent human fibroblasts have increased frequency and length of primary cilia. Levels of the negative ciliary regulator CP110 were reduced in senescent cells, as were levels of key elements of the Hedgehog pathway. Hedgehog inhibition reduced proliferation in young cells with increased cilium length accompanying cell cycle arrest suggesting a regulatory function for Hedgehog in primary ciliation. Depletion of CP110 in young cell populations increased ciliation frequencies and reduced cell proliferation. These data suggest that primary cilia are potentially novel determinants of the reduced cellular proliferation that initiates senescence. PMID:25486364

  19. Ciliary abnormalities in senescent human fibroblasts impair proliferative capacity

    Science.gov (United States)

    Breslin, Loretta; Prosser, Suzanna L; Cuffe, Sandra; Morrison, Ciaran G

    2014-01-01

    Somatic cells senesce in culture after a finite number of divisions indefinitely arresting their proliferation. DNA damage and senescence increase the cellular number of centrosomes, the 2 microtubule organizing centers that ensure bipolar mitotic spindles. Centrosomes also provide the basal body from which primary cilia extend to sense and transduce various extracellular signals, notably Hedgehog. Primary cilium formation is facilitated by cellular quiescence a temporary cell cycle exit, but the impact of senescence on cilia is unknown. We found that senescent human fibroblasts have increased frequency and length of primary cilia. Levels of the negative ciliary regulator CP110 were reduced in senescent cells, as were levels of key elements of the Hedgehog pathway. Hedgehog inhibition reduced proliferation in young cells with increased cilium length accompanying cell cycle arrest suggesting a regulatory function for Hedgehog in primary ciliation. Depletion of CP110 in young cell populations increased ciliation frequencies and reduced cell proliferation. These data suggest that primary cilia are potentially novel determinants of the reduced cellular proliferation that initiates senescence. PMID:25486364

  20. Diagnosis by ultrastructural study of primary ciliary dyskinesia

    Directory of Open Access Journals (Sweden)

    Melgarejo-Moreno P

    2015-01-01

    Full Text Available Introduction and objective: Primary ciliary dyskinesia (PCD, also known as ciliary immotility (SIC syndrome is an inherited disorder that includes a group of diseases in which respiratory cilia are immobile, ciliary movement is dyskinetic and ineffective or no cilia . The aim of this study is to determine the ciliary ultrastructure in patients with suspected DCP. Method: In 8 patients with suspected DCP nasal mucosa biopsy is performed with endoscopy at the inferior turbinate in the middle third by the ENT service under local anesthesia. Results: Of the 8 cases studied in 2 cases no ciliary ultrastructural level defects were found. In two cases with abnormal ciliary ultrastructure is present Kartagener syndrome. In a case no cilia were observed in the nasal mucosa. Discussion: The DCP and SIC are synonymous terms from clinical and pathogenetic view: immobility and dyskinesia lead to an absence of mucociliary transport, stasis of respiratory secretions with their consequences: chronic infections of lower respiratory tract and from birth . The most common ultrastructural defect is the total or partial absence of dynein. Conclusions: The ultrastructural study allows the diagnosis of PCD because genetic diagnosis is complicated and therefore get an early diagnosis of this condition which serves to improve the morbidity and mortality of these patients.

  1. [Mesectodermal leiomyoma. Unusual tumor of the ciliary body].

    Science.gov (United States)

    Rentería-Ruiz, Nancy Paulina; de Wit-Carter, Guillermo; Villaseñor-Diez, Jaime; Flores-Estrada, José Javier; Rodríguez-Reyes, Abelardo Antonio

    2014-01-01

    Antecedentes: el leiomioma mesoectodérmico es un tumor benigno excepcional que se origina en el músculo liso del cuerpo ciliar y deriva de la cresta neural. Caso clínico: se comunica el caso de una mujer de 35 años, con disminución de la agudeza visual y visión borrosa de 2 meses de evolución en el ojo derecho. El diagnóstico presuncional clínico e imagenológico fue: adenoma del epitelio no pigmentado del cuerpo ciliar, por lo que se resecó quirúrgicamente. Microscópicamente, el tumor estaba formado por células de núcleos redondos de escaso citoplasma sin atipia ni mitosis, dispuestas en una matriz fibrilar. Los inmunomarcadores para vimentina, actina músculo específica, actina de músculo liso y calponina fueron todos positivos en el citoplasma de las células neoplásicas, excepto de los inmunomarcadores para la proteína ácida gliofibrilar y la proteína S-100 que resultaron negativos en la misma población celular. Conclusiones: el leiomioma mesoectodérmico del cuerpo ciliar es un tumor benigno de músculo liso extremadamente raro en esta localización. Hasta el momento, sólo hay 25 casos informados en la bibliografía médica y su principal diagnóstico diferencial es melanoma uveal, motivo por el que algunos ojos se enuclearon. Los estudios de ultrabiomicroscopia y ecografía modos A y B son útiles en la evaluación; sin embargo, es obligado el estudio microscópico con tinciones de rutina, y el uso de marcadores inmunohistoquímicos, como los utilizados en este caso para establecer la naturaleza del músculo liso de esta neoplasia y descartar algunas otras, como el melanoma.

  2. Ciliary and non-ciliary expression and function of PACRG during vertebrate development

    Directory of Open Access Journals (Sweden)

    Thumberger Thomas

    2012-08-01

    Full Text Available Abstract Background Park2-co-regulated gene (PACRG is evolutionarily highly conserved from green algae to mammals. In Chlamydomonas and trypanosomes, the PACRG protein associates with flagella. Loss of PACRG results in shortened or absent flagella. In mouse the PACRG protein is required for spermatogenesis. The purpose of the present study was to analyze (1 the expression patterns of PACRG during vertebrate embryogenesis, and (2 whether the PACRG protein was required for left-right (LR axis specification through cilia-driven leftward flow in Xenopus laevis. Methods PACRG cDNAs were cloned and expression was analyzed during early embryonic development of Xenopus, mouse, rabbit and zebrafish. Antisense morpholino oligonucleotide (MO mediated gene knockdown was applied in Xenopus to investigate LR development at the level of tissue morphology, leftward flow and asymmetric marker gene expression, using timelapse videography, scanning electron microscopy (SEM and whole-mount in situ hybridization. Results were statistically evaluated using Wilcoxon paired and χ2 tests. Results PACRG mRNA expression was found in cells and tissues harboring cilia throughout the vertebrates. Highly localized expression was also detected in the brain. During early development, PACRG was specifically localized to epithelia where leftward flow arises, that is, the gastrocoel roof plate (GRP in Xenopus, the posterior notochord (PNC in mammals and Kupffer’s vesicle (KV in zebrafish. Besides its association with ciliary axonemes, subcellular localization of PACRG protein was found around the nucleus and in a spotty pattern in the cytoplasm. A green fluorescent protein (GFP fusion construct preferentially labeled cilia, rendering PACRG a versatile marker for live imaging. Loss-of-function in the frog resulted dose dependently in LR, neural tube closure and gastrulation defects, representing ciliary and non-ciliary functions of PACRG. Conclusions The PACRG protein is a novel

  3. Ciliary genes are down-regulated in bronchial tissue of primary ciliary dyskinesia patients.

    Directory of Open Access Journals (Sweden)

    Maciej Geremek

    Full Text Available Primary ciliary dyskinesia (PCD is a rare, genetically heterogeneous disease characterized by recurrent respiratory tract infections, sinusitis, bronchiectasis and male infertility. The pulmonary phenotype in PCD is caused by the impaired motility of cilia in the respiratory epithelium, due to ultrastructural defects of these organelles. We hypothesized that defects of multi-protein ciliary complexes should be reflected by gene expression changes in the respiratory epithelium. We have previously found that large group of genes functionally related to cilia share highly correlated expression pattern in PCD bronchial tissue. Here we performed an explorative analysis of differential gene expression in the bronchial tissue from six PCD patients and nine non-PCD controls, using Illumina HumanRef-12 Whole Genome BeadChips. We observed 1323 genes with at least 2-fold difference in the mean expression level between the two groups (t-test p-value <0.05. Annotation analysis showed that the genes down-regulated in PCD biopsies (602 were significantly enriched for terms related to cilia, whereas the up-regulated genes (721 were significantly enriched for terms related to cell cycle and mitosis. We assembled a list of human genes predicted to encode ciliary proteins, components of outer dynein arms, inner dynein arms, radial spokes, and intraflagellar transport proteins. A significant down-regulation of the expression of genes from all the four groups was observed in PCD, compared to non-PCD biopsies. Our data suggest that a coordinated down-regulation of the ciliome genes plays an important role in the molecular pathomechanism of PCD.

  4. CCDC103 mutations cause primary ciliary dyskinesia by disrupting assembly of ciliary dynein arms

    Science.gov (United States)

    Panizzi, Jennifer R.; Becker-Heck, Anita; Castleman, Victoria H.; Al-Mutairi, Dalal; Liu, Yan; Loges, Niki T.; Pathak, Narendra; Austin-Tse, Christina; Sheridan, Eamonn; Schmidts, Miriam; Olbrich, Heike; Werner, Claudius; Häffner, Karsten; Hellman, Nathan; Chodhari, Rahul; Gupta, Amar; Kramer-Zucker, Albrecht; Olale, Felix; Burdine, Rebecca D.; Schier, Alexander F.; O’Callaghan, Christopher; Chung, Eddie MK; Reinhardt, Richard; Mitchison, Hannah M.; King, Stephen M.; Omran, Heymut; Drummond, Iain A.

    2012-01-01

    Cilia are essential for fertilization, respiratory clearance, cerebrospinal fluid circulation, and to establish laterality1. Cilia motility defects cause Primary Ciliary Dyskinesia (PCD, MIM 242650), a disorder affecting 1:15-30,000 births. Cilia motility requires the assembly of multisubunit dynein arms that drive cilia bending2. Despite progress in understanding the genetic basis of PCD, mutations remain to be identified for several PCD linked loci3. Here we show that the zebrafish cilia paralysis mutant schmalhanstn222 (smh) mutant encodes the coiled-coil domain containing 103 protein (Ccdc103), a foxj1a regulated gene. Screening 146 unrelated PCD families identified patients in six families with reduced outer dynein arms, carrying mutations in CCDC103. Dynein arm assembly in smh mutant zebrafish was rescued by wild-type but not mutant human CCDC103. Chlamydomonas Ccdc103 functions as a tightly bound, axoneme-associated protein. The results identify Ccdc103 as a novel dynein arm attachment factor that when mutated causes Primary Ciliary Dyskinesia. PMID:22581229

  5. A ternary complex comprising transportin1, Rab8 and the ciliary targeting signal directs proteins to ciliary membranes

    Science.gov (United States)

    Madugula, Viswanadh

    2016-01-01

    ABSTRACT The sensory functions of cilia are dependent on the enrichment of cilium-resident proteins. Although it is known that ciliary targeting signals (CTSs) specifically target ciliary proteins to cilia, it is still unclear how CTSs facilitate the entry and retention of cilium-resident proteins at the molecular level. We found that non-ciliary membrane reporters can passively diffuse into cilia through the lateral transport pathway, and the translocation of membrane reporters through the ciliary diffusion barrier is facilitated by importin binding motifs and domains. Screening known CTSs of ciliary membrane residents uncovered that fibrocystin, photoreceptor retinol dehydrogenase, rhodopsin and retinitis pigmentosa 2 interact with transportin1 (TNPO1) through previously identified CTSs. We further discovered that a new ternary complex, comprising TNPO1, Rab8 and a CTS, can assemble or disassemble under the guanine nucleotide exchange activity of Rab8. Our study suggests a new mechanism in which the TNPO1–Rab8–CTS complex mediates selective entry into and retention of cargos within cilia. PMID:27633000

  6. Conservation of ciliary proteins in plants with no cilia

    Directory of Open Access Journals (Sweden)

    Hodges Matthew E

    2011-12-01

    Full Text Available Abstract Background Eukaryotic cilia are complex, highly conserved microtubule-based organelles with a broad phylogenetic distribution. Cilia were present in the last eukaryotic common ancestor and many proteins involved in cilia function have been conserved through eukaryotic diversification. However, cilia have also been lost multiple times in different lineages, with at least two losses occurring within the land plants. Whereas all non-seed plants produce cilia for motility of male gametes, some gymnosperms and all angiosperms lack cilia. During these evolutionary losses, proteins with ancestral ciliary functions may be lost or co-opted into different functions. Results Here we identify a core set of proteins with an inferred ciliary function that are conserved in ciliated eukaryotic species. We interrogate this genomic dataset to identify proteins with a predicted ancestral ciliary role that have been maintained in non-ciliated land plants. In support of our prediction, we demonstrate that several of these proteins have a flagellar localisation in protozoan trypanosomes. The phylogenetic distribution of these genes within the land plants indicates evolutionary scenarios of either sub- or neo-functionalisation and expression data analysis shows that these genes are highly expressed in Arabidopsis thaliana pollen cells. Conclusions A large number of proteins possess a phylogenetic ciliary profile indicative of ciliary function. Remarkably, many genes with an ancestral ciliary role are maintained in non-ciliated land plants. These proteins have been co-opted to perform novel functions, most likely before the loss of cilia, some of which appear related to the formation of the male gametes.

  7. The morphology, topography and cytoarchitectonics of the ciliary ganglion in the domestic turkey (Meleagris gallopavo domesticus).

    Science.gov (United States)

    Radzimirska, Małgorzata

    2003-11-01

    The ciliary ganglion of the domestic turkey (Meleagris gallopavo domesticus) is located between the posterior wall of the eyeball and the optic nerve. It is closely connected with the oculomotor nerve; in particular with its inferior branch. The ganglion has a cask-like shape and is adjacent to the inferior branch of the oculomotor nerve. From this ganglion postganglionic fibres emerge which are arranged in two fasciculi. These are termed the long ciliary nerves and the short ciliary nerves. A cross-section of the ciliary ganglion revealed two populations of cells: small ones - choroid cells and large ones - ciliary cells.

  8. Prostaglandin induces the expression of matrix metalloproteinase-1 In ciliary melanocytes

    Institute of Scientific and Technical Information of China (English)

    WANG Ning-li; LU Qing-jun; LI Jun-hong; WANG Ling

    2008-01-01

    Background Latanoprost,a prostaglandin F2a analog,has been shown to be an effective intraocular pressure lowering agent which acts by inducing ciliary muscle cells to synthesise matrix metalloproteinases.However,the response of ciliary melanocytes to latanoprost has never been reported.This research has investigated the ability of latanoprost to induce matrix metalloproteinase-1 expression in human ciliary melanocytes,and thereby advance the understanding of the mechanism of PGF2a in decreasing Intraocular pressure.Methods In vitro human ciliary melanocytes were treated for 48 hours with five different concentrations of latanoprost (100,150,200,500,and 1000 nmol/L).Ciliary melanocytes treated with 0.01% ethanel(vehicle)were used as a control.The expression of matrix metalloproteinase-1 in ciliary melanocytes was determined by Western blotting and immunofluorescent staining.Results Western blotting showed that the expression of matrix metalloproteinase-1 in ciliary melanocytes was induced by latanoprost,and the level of expression was dependent on the concentration of latanoprost in the culture medium.Immunofluorescent staining showed that matrix metalloproteinase-1 was confined to the ciliary melanocyte cytoplasm.Conclusions Latanoprost induced the expression of matrix metalloproteinase-1 in human ciliary melanocytes in a dose-dependent manner.Ciliary melanocytes,as well as ciliary muscle cells,may also play an important role in uveoscleral outflow modulation.

  9. Development of a ciliary muscle-driven accommodating intraocular lens

    NARCIS (Netherlands)

    Hermans, Erik A.; Terwee, Thom T.; Koopmans, Steven A.; Dubbelman, Michiel; van der Heijde, Rob G. L.; Heethaar, Rob M.

    2008-01-01

    PURPOSE: To develop a ciliary muscle-driven accommodating intraocular lens (IOL) that has a large and predictable range of variable power as a step toward spectacle independence. SETTING: Department of Physics and Medical Technology, VU University Medical Center, Amsterdam, The Netherlands. METHODS:

  10. Ciliary photoreceptors in the cerebral eyes of a protostome larva

    Directory of Open Access Journals (Sweden)

    Passamaneck Yale J

    2011-03-01

    Full Text Available Abstract Background Eyes in bilaterian metazoans have been described as being composed of either ciliary or rhabdomeric photoreceptors. Phylogenetic distribution, as well as distinct morphologies and characteristic deployment of different photopigments (ciliary vs. rhabdomeric opsins and transduction pathways argue for the co-existence of both of these two photoreceptor types in the last common bilaterian ancestor. Both receptor types exist throughout the Bilateria, but only vertebrates are thought to use ciliary photoreceptors for directional light detection in cerebral eyes, while all other invertebrate bilaterians studied utilize rhabdomeric photoreceptors for this purpose. In protostomes, ciliary photoreceptors that express c-opsin have been described only from a non-visual deep-brain photoreceptor. Their homology with vertebrate rods and cones of the human eye has been hypothesized to represent a unique functional transition from non-visual to visual roles in the vertebrate lineage. Results To test the hypothesis that protostome cerebral eyes employ exclusively rhabdomeric photoreceptors, we investigated the ultrastructure of the larval eyes in the brachiopod Terebratalia transversa. We show that these pigment-cup eyes consist of a lens cell and a shading pigment cell, both of which are putative photoreceptors, deploying a modified, enlarged cilium for light perception, and have axonal connections to the larval brain. Our investigation of the gene expression patterns of c-opsin, Pax6 and otx in these eyes confirms that the larval eye spots of brachiopods are cerebral eyes that deploy ciliary type photoreceptors for directional light detection. Interestingly, c-opsin is also expressed during early embryogenesis in all potential apical neural cells, becoming restricted to the anterior neuroectoderm, before expression is initiated in the photoreceptor cells of the eyes. Coincident with the expression of c-opsin in the presumptive neuroectoderm

  11. 视乳头色素细胞瘤的临床表现和随诊%Clinical manifestation and follow-up of melanocytoma of the optic disc

    Institute of Scientific and Technical Information of China (English)

    张承芬; 董方田; 陈有信; 杜虹; 韩宝玲

    2009-01-01

    Objective To investigate the clinical manifestation and follow-up of melanocytoma of the optic disc. Methods Retrospective case series study was taken to study the clinical data of the cases with melanocytoma of the optic disc at Peking Union Medical College Hospital from 1978 to 2007. The examination included direct and indirect ophthalmoscopy, fundus photography, fundus fluorescein angiography (FFA), fundus indocyanine green angiography (ICGA), optical coherence tomography (OCT) and B-scan. The clinical manifestation and the outcome in follow-up period were analyzed. Results Ten cases aged 31-58 years (mean 43 years) were included in this study. Among them, 9 cases were male, 1case was female. All cases were unilateral involved, 5 cases were right eye, 5 cases were left eye. The distribution of the visual acuity was follows: light perception in 2 eyes, 20/200 in one eye, 20/20 or above in 7 eyes. The tumor mass at the optic disc with extensive serous detachment of retina was detected on B-scan in one eye. The benign melanocytoma was diagnosed by vitreous fine needle biopsy for this eye. In other case, the tumor was found to be enlarged, visual field defect deteriorated, pigment granules appeared in the vitreous adjacent to the tumor during on year follow-up period. On the patient request, enucleation was performed to rule out the malignancy of the tumor. Pathologic study showed that the tumor cells were active, but benign state. The tumor size and visual acuity in other eight eyes remained stable in the follow-up period with one to 18 years (mean 3.5 years). On FFA and ICGA, bilateral choroidal nevi were found in one eye. Conclusions Melanocytoma of the optic disc is a benign tumor that rarely causes visual impairment. However, visual acuity may be impaired due to enlargement of the tumor or necrosis in tumor. It is important to follow-up every case with melanocytoma of the optic disc as long as pessible.%目的 探讨视乳头色素细胞瘤的临床表现

  12. Up to date on primary ciliary dyskinesia in children.

    Science.gov (United States)

    Pifferi, Massimo; Di Cicco, Maria; Piras, Martina; Cangiotti, Angela M; Saggese, Giuseppe

    2013-10-01

    Primary ciliary dyskinesia (PCD) is a congenital, clinically and ultrastructurally heterogeneous disease due to abnormal structure and/or function of cilia, with impaired mucociliary transport leading to several respiratory disorders. PCD can be diagnosed by the combination of thorough clinical examination with functional and ultrastructural analysis of the cilia. This paper shows progresses in PCD diagnosis obtained by ciliogenesis in culture evaluation of ciliated respiratory cells and by genetic analysis of mutations in candidate genes. Moreover, since to date no specific treatments are available to correct the ciliary dysfunction, the paper shows the proper therapeutical approach by the use of respiratory physiotherapy and regular exercise to favour airways clearance, by antibiotics administration to control acute airway infections. Macrolides administration as antiinflammatory option is suggested. PMID:23973004

  13. Disruption of Bardet-Biedl syndrome ciliary proteins perturbs planar cell polarity in vertebrates.

    Science.gov (United States)

    Ross, Alison J; May-Simera, Helen; Eichers, Erica R; Kai, Masatake; Hill, Josephine; Jagger, Daniel J; Leitch, Carmen C; Chapple, J Paul; Munro, Peter M; Fisher, Shannon; Tan, Perciliz L; Phillips, Helen M; Leroux, Michel R; Henderson, Deborah J; Murdoch, Jennifer N; Copp, Andrew J; Eliot, Marie-Madeleine; Lupski, James R; Kemp, David T; Dollfus, Hélène; Tada, Masazumi; Katsanis, Nicholas; Forge, Andrew; Beales, Philip L

    2005-10-01

    The evolutionarily conserved planar cell polarity (PCP) pathway (or noncanonical Wnt pathway) drives several important cellular processes, including epithelial cell polarization, cell migration and mitotic spindle orientation. In vertebrates, PCP genes have a vital role in polarized convergent extension movements during gastrulation and neurulation. Here we show that mice with mutations in genes involved in Bardet-Biedl syndrome (BBS), a disorder associated with ciliary dysfunction, share phenotypes with PCP mutants including open eyelids, neural tube defects and disrupted cochlear stereociliary bundles. Furthermore, we identify genetic interactions between BBS genes and a PCP gene in both mouse (Ltap, also called Vangl2) and zebrafish (vangl2). In zebrafish, the augmented phenotype results from enhanced defective convergent extension movements. We also show that Vangl2 localizes to the basal body and axoneme of ciliated cells, a pattern reminiscent of that of the BBS proteins. These data suggest that cilia are intrinsically involved in PCP processes.

  14. Myristoylated CIL-7 regulates ciliary extracellular vesicle biogenesis.

    Science.gov (United States)

    Maguire, Julie E; Silva, Malan; Nguyen, Ken C Q; Hellen, Elizabeth; Kern, Andrew D; Hall, David H; Barr, Maureen M

    2015-08-01

    The cilium both releases and binds to extracellular vesicles (EVs). EVs may be used by cells as a form of intercellular communication and mediate a broad range of physiological and pathological processes. The mammalian polycystins (PCs) localize to cilia, as well as to urinary EVs released from renal epithelial cells. PC ciliary trafficking defects may be an underlying cause of autosomal dominant polycystic kidney disease (PKD), and ciliary-EV interactions have been proposed to play a central role in the biology of PKD. In Caenorhabditis elegans and mammals, PC1 and PC2 act in the same genetic pathway, act in a sensory capacity, localize to cilia, and are contained in secreted EVs, suggesting ancient conservation. However, the relationship between cilia and EVs and the mechanisms generating PC-containing EVs remain an enigma. In a forward genetic screen for regulators of C. elegans PKD-2 ciliary localization, we identified CIL-7, a myristoylated protein that regulates EV biogenesis. Loss of CIL-7 results in male mating behavioral defects, excessive accumulation of EVs in the lumen of the cephalic sensory organ, and failure to release PKD-2::GFP-containing EVs to the environment. Fatty acylation, such as myristoylation and palmitoylation, targets proteins to cilia and flagella. The CIL-7 myristoylation motif is essential for CIL-7 function and for targeting CIL-7 to EVs. C. elegans is a powerful model with which to study ciliary EV biogenesis in vivo and identify cis-targeting motifs such as myristoylation that are necessary for EV-cargo association and function. PMID:26041936

  15. Meiotic chromosome behaviour in Cenchrus ciliaris

    Directory of Open Access Journals (Sweden)

    N. C. Visser

    1998-12-01

    Full Text Available A basic chromosome number of x = 9 has been confirmed for Cenchrus ciliaris L. Polyploidy is common and levels vary from tetraploid to hexaploid. Aneuploidv is reported for a single specimen, where two chromosomes of a single genome were lost. Various meiotic irregularities were observed. The highest incidence of meiotic abnormalities was observed in the pentaploid specimens. This was attributed to their uneven polyploid level All specimens varied from segmental alloploid to alloploid.

  16. Photosynthesis of Digitaria ciliaris during repeated soil drought and rewatering

    Institute of Scientific and Technical Information of China (English)

    YaYong Luo; XueYong Zhao; JingHui Zhang; YuLin Li; XiaoAn Zuo; DianChao Sun

    2015-01-01

    The ability of psammophyte photosynthesis to withstand and recover from severe droughts is crucial for vegetation sta-bility in semi-arid sandy lands. The responses of gas exchange and chlorophyll fluorescence of an annual grass, Digitaria ciliaris, were measured through three soil drought and rewatering cycles. Results showed that the net photosynthesis rate (Pn) decreased by 92%, 95%, and 63%at end of the three drought periods, respectively, water use efficiency (WUE) de-creased by 67%, 54%, and 48%, while the constant intercellular CO2 concentration (Ci) increased by 1.08, 0.88, and 0.45 times. During those three cycles, the trapping probability with no dark adaptation (Fv′/Fm′) decreased by 55%, 51%, and 9%, the electron transport per cross section (ET0′/CS0′) decreased by 63%, 42%, and 18%, and the dissipation per cross section (DI0′/CS0′) increased by 97%, 96%, and 21%. These results indicated that D. ciliaris was subjected to photoinhi-bition and some non-stomatal limitation of photosynthesis under drought. However, after four days of rewatering, its photosynthetic characteristics were restored to control values. This capability to recover from drought may contribute to making the plant's use of water as efficient as possible. Furthermore, the photosynthesis decreased more slowly in the subsequent drought cycles than in the first cycle, allowing D. ciliaris to enhance its future drought tolerance after drought hardening. Thus, it acclimatizes itself to repeated soil drought.

  17. Diagnosis and treatment of primary melanocytoma in central nervous system%中枢神经系统原发性黑色素细胞瘤的治疗体会

    Institute of Scientific and Technical Information of China (English)

    周渊; 王汉东; 马驰原; 成惠林; 樊友武; 刘开东; 励宁; 何进

    2014-01-01

    Objective There are differences in the diagnosis and treatment of primary melanocytoma in central nervous sys -tem.The article was to investigate the experience of its diagnosis and treatment . Methods Retrospective analysis were made on the clinical data of 14 cases with primary melanocytoma in central nervous system ( CNS) from January 1999 to December 2012, among which were 5 males and 9 females.The incidence ages were 14-52, average 32.7.The course of disease ranged from half a month to 19 years, geometric average 7.9 months.5 cases recurred and 9 cases occurred first.10 cases were intracranial and 4 were intraspinal. Results 14 patients underwent surgery and had pathologic diagnosis of melanocytoma .Total resection was performed in 7 patients, subtotal resection in 3, and partial resection in 1.Immunohistochemical study showed , in all cases, S-100 and HMB-45 were positive, GFAP and EMA were negative .Vimentin was positive in 8 cases and MelanA positive in 5 cases.12 cases recovered well and dis-charged except for paraplegia and facial paralysis in 1 case each. Conclusion Primary melanocytoma in CNS is very rare .Diagnosis is based on intraoperative findings , surgical pathology and immunohistochemistry results .Surgery is the primary therapy and early total resection is advocated .Adjuvant radiotherapy can reduce the recurrence rate .%目的:中枢神经系统原发性黑色素细胞瘤罕见。文中探讨其治疗经验。方法回顾性分析南京军区南京总医院神经外科1999年1月至2012年12月收治的14例中枢神经系统原发性黑色素细胞瘤患者的临床资料,其中男5例,女9例,发病年龄14~52岁,平均年龄(32.7±10.7)岁。病程15 d至19年,中位值7个月。复发5例,首发9例。发生于颅内10例,发生于椎管内4例。 MRI检查结果示病灶T1WI相呈等、高信号,T2WI相呈等、低信号。病理免疫组化结果:所有患者S-100(+)、HMB45

  18. Culture of primary ciliary dyskinesia epithelial cells at air-liquid interface can alter ciliary phenotype but remains a robust and informative diagnostic aid.

    Directory of Open Access Journals (Sweden)

    Robert A Hirst

    Full Text Available BACKGROUND: The diagnosis of primary ciliary dyskinesia (PCD requires the analysis of ciliary function and ultrastructure. Diagnosis can be complicated by secondary effects on cilia such as damage during sampling, local inflammation or recent infection. To differentiate primary from secondary abnormalities, re-analysis of cilia following culture and re-differentiation of epithelial cells at an air-liquid interface (ALI aids the diagnosis of PCD. However changes in ciliary beat pattern of cilia following epithelial cell culture has previously been described, which has brought the robustness of this method into question. This is the first systematic study to evaluate ALI culture as an aid to diagnosis of PCD in the light of these concerns. METHODS: We retrospectively studied changes associated with ALI-culture in 158 subjects referred for diagnostic testing at two PCD centres. Ciliated nasal epithelium (PCD n = 54; non-PCD n  111 was analysed by high-speed digital video microscopy and transmission electron microscopy before and after culture. RESULTS: Ciliary function was abnormal before and after culture in all subjects with PCD; 21 PCD subjects had a combination of static and uncoordinated twitching cilia, which became completely static following culture, a further 9 demonstrated a decreased ciliary beat frequency after culture. In subjects without PCD, secondary ciliary dyskinesia was reduced. CONCLUSIONS: The change to ciliary phenotype in PCD samples following cell culture does not affect the diagnosis, and in certain cases can assist the ability to identify PCD cilia.

  19. Computational modelling elucidates the mechanism of ciliary regulation in health and disease

    Directory of Open Access Journals (Sweden)

    Hundhausen Christian

    2011-09-01

    Full Text Available Abstract Background Ciliary dysfunction leads to a number of human pathologies, including primary ciliary dyskinesia, nephronophthisis, situs inversus pathology or infertility. The mechanism of cilia beating regulation is complex and despite extensive experimental characterization remains poorly understood. We develop a detailed systems model for calcium, membrane potential and cyclic nucleotide-dependent ciliary motility regulation. Results The model describes the intimate relationship between calcium and potassium ionic concentrations inside and outside of cilia with membrane voltage and, for the first time, describes a novel type of ciliary excitability which plays the major role in ciliary movement regulation. Our model describes a mechanism that allows ciliary excitation to be robust over a wide physiological range of extracellular ionic concentrations. The model predicts the existence of several dynamic modes of ciliary regulation, such as the generation of intraciliary Ca2+ spike with amplitude proportional to the degree of membrane depolarization, the ability to maintain stable oscillations, monostable multivibrator regimes, all of which are initiated by variability in ionic concentrations that translate into altered membrane voltage. Conclusions Computational investigation of the model offers several new insights into the underlying molecular mechanisms of ciliary pathologies. According to our analysis, the reported dynamic regulatory modes can be a physiological reaction to alterations in the extracellular environment. However, modification of the dynamic modes, as a result of genetic mutations or environmental conditions, can cause a life threatening pathology.

  20. Discinesia ciliar primária Primary ciliary dyskinesia

    Directory of Open Access Journals (Sweden)

    JOSÉ WELLINGTON ALVES DOS SANTOS

    2001-09-01

    Full Text Available Discinesia ciliar primária é uma doença autossômica recessiva caracterizada pela história de infecções repetidas do trato respiratório superior e inferior, otite média, bronquite e rinossinusite, associada a situs inversus na metade dos casos. O diagnóstico é estabelecido pela análise ciliar ultra-estrutural de espécimes respiratórios, após a exclusão inicial de outras doenças, como fibrose cística, deficiência de alfa-1-antitripsina, imunodeficiências (IgG, neutrófilos e complemento e síndrome de Young. O propósito deste artigo é revisar os achados clínicos, o diagnóstico e o manejo da discinesia ciliar primária, incluindo um fluxograma diagnóstico.Primary ciliary dyskinesia is an autosomal recessive disorder characterized by a history of recurrent upper and lower respiratory tract infections with chronic otitis media, bronchitis and rhinosinusitis, associated with situs inversus in 50% of cases. The diagnosis is established by ciliary ultrastructural analysis of respiratory specimens, after ruling out some disorders as cystic fibrosis, alpha-1 anti-trypsin deficiency, immune deficiencies (IgG, neutrophils and complement and Young's syndrome. The purpose of this paper is to review the clinical features, diagnosis and management of primary ciliary dyskinesia, including a diagnostic algorithm.

  1. Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila.

    Science.gov (United States)

    Chen, Jieyan V; Kao, Ling-Rong; Jana, Swadhin C; Sivan-Loukianova, Elena; Mendonça, Susana; Cabrera, Oscar A; Singh, Priyanka; Cabernard, Clemens; Eberl, Daniel F; Bettencourt-Dias, Monica; Megraw, Timothy L

    2015-10-26

    Cilia are essential for cell signaling and sensory perception. In many cell types, a cytoskeletal structure called the ciliary rootlet links the cilium to the cell body. Previous studies indicated that rootlets support the long-term stability of some cilia. Here we report that Drosophila melanogaster Rootletin (Root), the sole orthologue of the mammalian paralogs Rootletin and C-Nap1, assembles into rootlets of diverse lengths among sensory neuron subtypes. Root mutant neurons lack rootlets and have dramatically impaired sensory function, resulting in behavior defects associated with mechanosensation and chemosensation. Root is required for cohesion of basal bodies, but the cilium structure appears normal in Root mutant neurons. We show, however, that normal rootlet assembly requires centrioles. The N terminus of Root contains a conserved domain and is essential for Root function in vivo. Ectopically expressed Root resides at the base of mother centrioles in spermatocytes and localizes asymmetrically to mother centrosomes in neuroblasts, both requiring Bld10, a basal body protein with varied functions. PMID:26483560

  2. Riding the wave of ependymal cilia: genetic susceptibility to hydrocephalus in primary ciliary dyskinesia.

    Science.gov (United States)

    Lee, Lance

    2013-09-01

    Congenital hydrocephalus is a relatively common and debilitating birth defect with several known physiological causes. Dysfunction of motile cilia on the ependymal cells that line the ventricular surface of the brain can result in hydrocephalus by hindering the proper flow of cerebrospinal fluid. As a result, hydrocephalus can be associated with primary ciliary dyskinesia, a rare pediatric syndrome resulting from defects in ciliary and flagellar motility. Although the prevalence of hydrocephalus in primary ciliary dyskinesia patients is low, it is a common hallmark of the disease in mouse models, suggesting that distinct genetic mechanisms underlie the differences in the development and physiology of human and mouse brains. Mouse models of primary ciliary dyskinesia reveal strain-specific differences in the appearance and severity of hydrocephalus, indicating the presence of genetic modifiers segregating in inbred strains. These models may provide valuable insight into the genetic mechanisms that regulate susceptibility to hydrocephalus under the conditions of ependymal ciliary dysfunction. PMID:23686703

  3. Automatic analysis of ciliary beat frequency using optical flow

    Science.gov (United States)

    Figl, Michael; Lechner, Manuel; Werther, Tobias; Horak, Fritz; Hummel, Johann; Birkfellner, Wolfgang

    2012-02-01

    Ciliary beat frequency (CBF) can be a useful parameter for diagnosis of several diseases, as e.g. primary ciliary dyskinesia. (PCD). CBF computation is usually done using manual evaluation of high speed video sequences, a tedious, observer dependent, and not very accurate procedure. We used the OpenCV's pyramidal implementation of the Lukas-Kanade algorithm for optical flow computation and applied this to certain objects to follow the movements. The objects were chosen by their contrast applying the corner detection by Shi and Tomasi. Discrimination between background/noise and cilia by a frequency histogram allowed to compute the CBF. Frequency analysis was done using the Fourier transform in matlab. The correct number of Fourier summands was found by the slope in an approximation curve. The method showed to be usable to distinguish between healthy and diseased samples. However there remain difficulties in automatically identifying the cilia, and also in finding enough high contrast cilia in the image. Furthermore the some of the higher contrast cilia are lost (and sometimes found) by the method, an easy way to distinguish the correct sub-path of a point's path have yet to be found in the case where the slope methods doesn't work.

  4. Cdc42 deficiency causes ciliary abnormalities and cystic kidneys.

    Science.gov (United States)

    Choi, Soo Young; Chacon-Heszele, Maria F; Huang, Liwei; McKenna, Sarah; Wilson, F Perry; Zuo, Xiaofeng; Lipschutz, Joshua H

    2013-09-01

    Ciliogenesis and cystogenesis require the exocyst, a conserved eight-protein trafficking complex that traffics ciliary proteins. In culture, the small GTPase Cdc42 co-localizes with the exocyst at primary cilia and interacts with the exocyst component Sec10. The role of Cdc42 in vivo, however, is not well understood. Here, knockdown of cdc42 in zebrafish produced a phenotype similar to sec10 knockdown, including tail curvature, glomerular expansion, and mitogen-activated protein kinase (MAPK) activation, suggesting that cdc42 and sec10 cooperate in ciliogenesis. In addition, cdc42 knockdown led to hydrocephalus and loss of photoreceptor cilia. Furthermore, there was a synergistic genetic interaction between zebrafish cdc42 and sec10, suggesting that cdc42 and sec10 function in the same pathway. Mice lacking Cdc42 specifically in kidney tubular epithelial cells died of renal failure within weeks of birth. Histology revealed cystogenesis in distal tubules and collecting ducts, decreased ciliogenesis in cyst cells, increased tubular cell proliferation, increased apoptosis, increased fibrosis, and led to MAPK activation, all of which are features of polycystic kidney disease, especially nephronophthisis. Taken together, these results suggest that Cdc42 localizes the exocyst to primary cilia, whereupon the exocyst targets and docks vesicles carrying ciliary proteins. Abnormalities in this pathway result in deranged ciliogenesis and polycystic kidney disease.

  5. Cdc42 deficiency causes ciliary abnormalities and cystic kidneys.

    Science.gov (United States)

    Choi, Soo Young; Chacon-Heszele, Maria F; Huang, Liwei; McKenna, Sarah; Wilson, F Perry; Zuo, Xiaofeng; Lipschutz, Joshua H

    2013-09-01

    Ciliogenesis and cystogenesis require the exocyst, a conserved eight-protein trafficking complex that traffics ciliary proteins. In culture, the small GTPase Cdc42 co-localizes with the exocyst at primary cilia and interacts with the exocyst component Sec10. The role of Cdc42 in vivo, however, is not well understood. Here, knockdown of cdc42 in zebrafish produced a phenotype similar to sec10 knockdown, including tail curvature, glomerular expansion, and mitogen-activated protein kinase (MAPK) activation, suggesting that cdc42 and sec10 cooperate in ciliogenesis. In addition, cdc42 knockdown led to hydrocephalus and loss of photoreceptor cilia. Furthermore, there was a synergistic genetic interaction between zebrafish cdc42 and sec10, suggesting that cdc42 and sec10 function in the same pathway. Mice lacking Cdc42 specifically in kidney tubular epithelial cells died of renal failure within weeks of birth. Histology revealed cystogenesis in distal tubules and collecting ducts, decreased ciliogenesis in cyst cells, increased tubular cell proliferation, increased apoptosis, increased fibrosis, and led to MAPK activation, all of which are features of polycystic kidney disease, especially nephronophthisis. Taken together, these results suggest that Cdc42 localizes the exocyst to primary cilia, whereupon the exocyst targets and docks vesicles carrying ciliary proteins. Abnormalities in this pathway result in deranged ciliogenesis and polycystic kidney disease. PMID:23766535

  6. Intraocular electro-optic lens with ciliary muscle controlled accommodation.

    Science.gov (United States)

    Doornaert, Dries; Glorieux, Christ; De Gersem, Herbert; Puers, Robert; Spileers, Werner; Blanckaert, Johan

    2013-01-01

    In this paper a concept is proposed of an intraocular lens implant with electro-optic accommodation of a variable-focus hybrid liquid-crystal-based lens. The dioptric strength of the lens is electronically controlled by a signal that is derived from the change of inductance of a sensing coil due to a marker implanted in the nearby contracting or decontracting ciliary muscle. Analytical, numerical and experimental results are reported on the dependency of the frequency of a Colpitts oscillator circuit on the location of a nearby conductive marker. A concept is also reported on the use as an electro-optic lens of a device based on a liquid crystal in planar alignment, which is held between a flat and a curved window coated with optically transparent and electrically conductive layers. PMID:24110406

  7. Genotypic Variation for Salinity Tolerance in Cenchrus ciliaris L.

    Science.gov (United States)

    Al-Dakheel, Abdullah J.; Hussain, M. Iftikhar

    2016-01-01

    Scarcity of irrigation water and increasing soil salinization has threatened the sustainability of forage production in arid and semi-arid region around the globe. Introduction of salt-tolerant perennial species is a promising alternative to overcome forage deficit to meet future livestock needs in salt-affected areas. This study presents the results of a salinity tolerance screening trial which was carried out in plastic pots buried in the open field for 160 buffelgrass (Cenchrus ciliaris L.) accessions for three consecutive years (2003–2005). The plastic pots were filled with sand, organic, and peat moss mix and were irrigated with four different quality water (EC 0, 10, 15, and 20 dS m−1). The results indicate that the average annual dry weights (DW) were in the range from 122.5 to 148.9 g/pot in control; 96.4–133.8 g/pot at 10 dS m−1; 65.6–80.4 g/pot at 15 dS m−1, and 55.4–65.6 g/pot at 20 dS m−1. The highest DW (148.9 g/pot) was found with accession 49 and the lowest with accession 23. Principle component analysis shows that PC-1 contributed 81.8% of the total variability, while PC-2 depicted 11.7% of the total variation among C. ciliaris accessions for DW. Hierarchical cluster analysis revealed that a number of accessions collected from diverse regions could be grouped into a single cluster. Accessions 3, 133, 159, 30, 23, 142, 141, 95, 49, 129, 124, and 127 were stable, salt tolerant, and produced good dry biomass yield. These accessions demonstrate sufficient salinity tolerance potential for promotion in marginal lands to enhance farm productivity and reduce rural poverty. PMID:27516762

  8. Genotypic Variation for Salinity Tolerance in Cenchrus ciliaris L.

    Science.gov (United States)

    Al-Dakheel, Abdullah J; Hussain, M Iftikhar

    2016-01-01

    Scarcity of irrigation water and increasing soil salinization has threatened the sustainability of forage production in arid and semi-arid region around the globe. Introduction of salt-tolerant perennial species is a promising alternative to overcome forage deficit to meet future livestock needs in salt-affected areas. This study presents the results of a salinity tolerance screening trial which was carried out in plastic pots buried in the open field for 160 buffelgrass (Cenchrus ciliaris L.) accessions for three consecutive years (2003-2005). The plastic pots were filled with sand, organic, and peat moss mix and were irrigated with four different quality water (EC 0, 10, 15, and 20 dS m(-1)). The results indicate that the average annual dry weights (DW) were in the range from 122.5 to 148.9 g/pot in control; 96.4-133.8 g/pot at 10 dS m(-1); 65.6-80.4 g/pot at 15 dS m(-1), and 55.4-65.6 g/pot at 20 dS m(-1). The highest DW (148.9 g/pot) was found with accession 49 and the lowest with accession 23. Principle component analysis shows that PC-1 contributed 81.8% of the total variability, while PC-2 depicted 11.7% of the total variation among C. ciliaris accessions for DW. Hierarchical cluster analysis revealed that a number of accessions collected from diverse regions could be grouped into a single cluster. Accessions 3, 133, 159, 30, 23, 142, 141, 95, 49, 129, 124, and 127 were stable, salt tolerant, and produced good dry biomass yield. These accessions demonstrate sufficient salinity tolerance potential for promotion in marginal lands to enhance farm productivity and reduce rural poverty. PMID:27516762

  9. The development of the larval nervous system, musculature and ciliary bands of Pomatoceros lamarckii (Annelida: heterochrony in polychaetes

    Directory of Open Access Journals (Sweden)

    Shimeld Sebastian M

    2006-10-01

    Full Text Available Abstract Background To understand the evolution of animals it is essential to have taxon sampling across a representative spread of the animal kingdom. With the recent rearrangement of most of the Bilateria into three major clades (Ecdysozoa, Lophotrochozoa and Deuterostomia it has become clear that the Lophotrochozoa are relatively poorly represented in our knowledge of animal development, compared to the Ecdysozoa and Deuterostomia. We aim to contribute towards redressing this balance with data on the development of the muscular, nervous and ciliary systems of the annelid Pomatoceros lamarckii (Serpulidae. We compare our data with other lophotrochozoans. Results P. lamarckii develops locomotory and feeding structures that enable it to become a swimming, planktotrophic larva within 24 hours. Formation of the trochophore includes development of a prototroch, metatroch and neurotroch, development of apical and posterior nervous elements at similar times, and development of musculature around the ciliary bands and digestive tract prior to development of any body wall muscles. The adult nervous and muscular systems are essentially preformed in the late larva. Interestingly, the muscular systems of the larvae and juvenile worms do not include the circular muscles of the body wall, which are considered to be plesiomorphic for annelids, although the possibility that circular muscles develop after these stages cannot be ruled out at this point. Conclusion A comparison between polychaetes shows variability in the timing (heterochrony of development of body wall muscles and elements of the nervous system. These heterochronies are one route for evolution of different life history strategies, such as adaptations to feeding requirements.

  10. Simultaneous sinus and lung infections in patients with primary ciliary dyskinesia

    DEFF Research Database (Denmark)

    Alanin, Mikkel Christian; Johansen, Helle Krogh; Aanaes, Kasper;

    2015-01-01

    Conclusion: The sinuses should be considered as a bacterial reservoir and a target for surgery and antibiotic treatment in patients with primary ciliary dyskinesia (PCD). The observed decrease in serum precipitating antibodies (precipitins) against Pseudomonas aeruginosa may indicate a beneficial...

  11. Measurement of ciliary flow generated on the surface of tracheal lumen

    Science.gov (United States)

    Kiyota, Koki; Ueno, Hironori; Ishikawa, Takuji; Numayama-Tsuruta, Keiko; Imai, Yohsuke; Omori, Toshihiro; Yamaguchi, Takami

    2012-11-01

    Although we consistently take air with virus and bacteria, these harmful substances are trapped on the surface of tracheal lumen and transported toward larynx from the trachea and bronchi by effective ciliary motion and swallowed it (clearance function). However, the 3-dimensional flow field generated by inhomogeneously distributed ciliary cells are largely unknown. In this study, we first succeeded to measure the ciliated cells' density by staining actin of the epithelial cells and tubulin of the cilia, respectively. Second, we analyzed the ciliary motion by labeling the tip of cilia with fluorescent particles, and tracking their movements to understand the mechanism of the flow generation. Last, in order to clarify the flow field induced by the ciliary motion, we measured the motion of tracer particles on the surface of tracheal epithelial cells by a confocal micro-PTV system. The results show that the mean velocity and the velocity disturbance decayed rapidly as the height from the epithelial cells were increased.

  12. Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes

    OpenAIRE

    WEI, QING; Xu, Qingwen; Zhang, Yuxia; Li, Yujie; Zhang, Qing; Hu, Zeng; Harris, Peter C.; Torres, Vicente E.; Ling, Kun; Hu, Jinghua

    2013-01-01

    Sensory organelle cilia play critical roles in mammalian embryonic development and tissue homeostasis. Intraflagellar transport (IFT) machinery is required for the assembly and maintenance of cilia. Yet how this large complex passes through the size-dependent barrier at the ciliary base remains enigmatic. Here we report that FBF1, a highly conserved transition fibre protein, is required for the ciliary import of assembled IFT particles at the cilia base. We cloned dyf-19, the C. elegans homol...

  13. Automated image analysis reveals the dynamic 3-dimensional organization of multi-ciliary arrays

    Directory of Open Access Journals (Sweden)

    Domenico F. Galati

    2016-01-01

    Full Text Available Multi-ciliated cells (MCCs use polarized fields of undulating cilia (ciliary array to produce fluid flow that is essential for many biological processes. Cilia are positioned by microtubule scaffolds called basal bodies (BBs that are arranged within a spatially complex 3-dimensional geometry (3D. Here, we develop a robust and automated computational image analysis routine to quantify 3D BB organization in the ciliate, Tetrahymena thermophila. Using this routine, we generate the first morphologically constrained 3D reconstructions of Tetrahymena cells and elucidate rules that govern the kinetics of MCC organization. We demonstrate the interplay between BB duplication and cell size expansion through the cell cycle. In mutant cells, we identify a potential BB surveillance mechanism that balances large gaps in BB spacing by increasing the frequency of closely spaced BBs in other regions of the cell. Finally, by taking advantage of a mutant predisposed to BB disorganization, we locate the spatial domains that are most prone to disorganization by environmental stimuli. Collectively, our analyses reveal the importance of quantitative image analysis to understand the principles that guide the 3D organization of MCCs.

  14. Force-inhibiting effect of Ser/Thr protein phosphatase 2A inhibitors on bovine ciliary muscle.

    OpenAIRE

    石田, 美織

    2015-01-01

    Ciliary muscle is a smooth muscle characterized by a rapid response to muscarinic receptor stimulation and sustained contraction. Although it is evident that these contractions are Ca(2+)-dependent, detailed molecular mechanisms are still unknown. In order to elucidate the role of Ser/Thr protein phosphatase 2A (PP2A) in ciliary muscle contraction, we examined the effects of okadaic acid and other PP2A inhibitors on contractions induced by carbachol (CCh) and ionomycin in bovine ciliary muscl...

  15. Diagnosing primary ciliary dyskinesia: an international patient perspective

    Science.gov (United States)

    Dunn Galvin, Audrey; Rubbo, Bruna; Masefield, Sarah; Copeland, Fiona; Manion, Michele; Rindlisbacher, Bernhard; Redfern, Beatrice; Lucas, Jane S.

    2016-01-01

    Primary ciliary dyskinesia (PCD) is a rare genetic disorder characterised by progressive sino-pulmonary disease, with symptoms starting soon after birth. A European Respiratory Society (ERS) Task Force aims to address disparities in diagnostics across Europe by providing evidence-based clinical practice guidelines. We aimed to identify challenges faced by patients when referred for PCD diagnostic testing. A patient survey was developed by patient representatives and healthcare specialists to capture experience. Online versions of the survey were translated into nine languages and completed in 25 countries. Of the respondents (n=365), 74% were PCD-positive, 5% PCD-negative and 21% PCD-uncertain/inconclusive. We then interviewed 20 parents/patients. Transcripts were analysed thematically. 35% of respondents visited their doctor more than 40 times with PCD-related symptoms prior to diagnostic referral. Furthermore, the most prominent theme among interviewees was a lack of PCD awareness among medical practitioners and failure to take past history into account, leading to delayed diagnosis. Patients also highlighted the need for improved reporting of results and a solution to the “inconclusive” diagnostic status. These findings will be used to advise the ERS Task Force guidelines for diagnosing PCD, and should help stakeholders responsible for improving existing services and expanding provision for diagnosis of this rare disease. PMID:27492837

  16. Protective Effects of Ciliary Neurotrophic Factor on Denervated Skeletal Muscle

    Institute of Scientific and Technical Information of China (English)

    黄仕龙; 王发斌; 洪光祥; 万圣祥; 康皓

    2002-01-01

    Summary: To study the effects of ciliary neurotrophic factor (CNTF) on denervated skeletal muscle atrophy and to find a new approach to ameliorate atrophy of denervated muscle, a model was estab lished by cutting the right sciatic nerve in 36 Wistar mice, with the left side serving as control. Then they were divided into two groups randomly. CNTF (1 U/ml) 0. 1 ml was injected into the right tib-ial muscle every day in experimental group, and saline was used into another group for comparison.The muscle wet weight, muscle total protein, Ca2+, physiological response and morphology were an alyzed on the 7th, 14th and 28th day after operation. Our results showed that compared to control group, there was a significant increase in muscle wet weight, total protein, Ca2+ , muscle fiber cross section area in CNTF group (P< 0. 05). CNTF could ameliorate the decrease of tetanic tension (PO), post-tetanic twitch potentiation (PTP), and the prolonged muscle relaxation time (RT)caused by denervation (P<0. 05). The motor end-plate areas 7 days and 14 days after denervation was similar (P>0. 05), but significantly larger 28 days after the denervation (P<0.05). Our re-sults suggest that CNTF exerts myotrophic effects by attenuating the morphological and functional changes associated with denervation of rat muscles and has protective effects on denervated muscle and motor end plate.

  17. PICADAR: a diagnostic predictive tool for primary ciliary dyskinesia

    Science.gov (United States)

    Behan, Laura; Dimitrov, Borislav D.; Kuehni, Claudia E.; Hogg, Claire; Carroll, Mary; Evans, Hazel J.; Goutaki, Myrofora; Harris, Amanda; Packham, Samantha; Walker, Woolf T.

    2016-01-01

    Symptoms of primary ciliary dyskinesia (PCD) are nonspecific and guidance on whom to refer for testing is limited. Diagnostic tests for PCD are highly specialised, requiring expensive equipment and experienced PCD scientists. This study aims to develop a practical clinical diagnostic tool to identify patients requiring testing. Patients consecutively referred for testing were studied. Information readily obtained from patient history was correlated with diagnostic outcome. Using logistic regression, the predictive performance of the best model was tested by receiver operating characteristic curve analyses. The model was simplified into a practical tool (PICADAR) and externally validated in a second diagnostic centre. Of 641 referrals with a definitive diagnostic outcome, 75 (12%) were positive. PICADAR applies to patients with persistent wet cough and has seven predictive parameters: full-term gestation, neonatal chest symptoms, neonatal intensive care admittance, chronic rhinitis, ear symptoms, situs inversus and congenital cardiac defect. Sensitivity and specificity of the tool were 0.90 and 0.75 for a cut-off score of 5 points. Area under the curve for the internally and externally validated tool was 0.91 and 0.87, respectively. PICADAR represents a simple diagnostic clinical prediction rule with good accuracy and validity, ready for testing in respiratory centres referring to PCD centres. PMID:26917608

  18. Arf4 is required for Mammalian development but dispensable for ciliary assembly.

    Directory of Open Access Journals (Sweden)

    John A Follit

    2014-02-01

    Full Text Available The primary cilium is a sensory organelle, defects in which cause a wide range of human diseases including retinal degeneration, polycystic kidney disease and birth defects. The sensory functions of cilia require specific receptors to be targeted to the ciliary subdomain of the plasma membrane. Arf4 has been proposed to sort cargo destined for the cilium at the Golgi complex and deemed a key regulator of ciliary protein trafficking. In this work, we show that Arf4 binds to the ciliary targeting sequence (CTS of fibrocystin. Knockdown of Arf4 indicates that it is not absolutely required for trafficking of the fibrocystin CTS to cilia as steady-state CTS levels are unaffected. However, we did observe a delay in delivery of newly synthesized CTS from the Golgi complex to the cilium when Arf4 was reduced. Arf4 mutant mice are embryonic lethal and die at mid-gestation shortly after node formation. Nodal cilia appeared normal and functioned properly to break left-right symmetry in Arf4 mutant embryos. At this stage of development Arf4 expression is highest in the visceral endoderm but we did not detect cilia on these cells. In the visceral endoderm, the lack of Arf4 caused defects in cell structure and apical protein localization. This work suggests that while Arf4 is not required for ciliary assembly, it is important for the efficient transport of fibrocystin to cilia, and also plays critical roles in non-ciliary processes.

  19. Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes.

    Science.gov (United States)

    Wei, Qing; Xu, Qingwen; Zhang, Yuxia; Li, Yujie; Zhang, Qing; Hu, Zeng; Harris, Peter C; Torres, Vicente E; Ling, Kun; Hu, Jinghua

    2013-01-01

    Sensory organelle cilia have critical roles in mammalian embryonic development and tissue homeostasis. Intraflagellar transport (IFT) machinery is required for the assembly and maintenance of cilia. Yet, how this large complex passes through the size-dependent barrier at the ciliary base remains enigmatic. Here we report that FBF1, a highly conserved transition fibre protein, is required for the ciliary import of assembled IFT particles at the ciliary base. We cloned dyf-19, the Caenorhabditis elegans homologue of human FBF1, in a whole-genome screen for ciliogenesis mutants. DYF-19 localizes specifically to transition fibres and interacts directly with the IFT-B component DYF-11/IFT54. Although not a structural component of transition fibres, DYF-19 is required for the transit of assembled IFT particles through the ciliary base. Furthermore, we found that human FBF1 shares conserved localization and function with its worm counterpart. We conclude that FBF1 is a key functional transition fibre component that actively facilitates the ciliary entry of assembled IFT machinery. PMID:24231678

  20. Fiber optic laser light scattering measurement of ciliary function of the fallopian tube

    Science.gov (United States)

    Halbert, Sheridan A.; Lim, Kap; Lee, Wylie I.

    1990-07-01

    A fiber-optic laser light-scattering system (FLS) for measuring ciliary function was evaluated by means of three sets of in vitro experiments. First, FLS performance was compared to that of a previously proven benchtop laser system (BLS). Using tissue excised from rabbit fallopian tubes, ciliary beat frequency (CBF) of each sample was measured with FLS and BLS. Paired CBF measurements showed excellent correlation between the two systems (r =0.93). Second, the FLS was used to evaluate the dependency of CBF on temperature (T) by using tissue sampies of rabbit oviductal fimbna. Regression analysis of CBF vs T showed a linear relationship over the range of 18-37°C for both individual samples (r =0.98) and pooled data from all experiments (r = 0.84). Fmally, the relalionship between CBF and ciliary ovum transport rate (TR) was tested by using T to modulate CBF of rabbit fimbria, in vitro. The relationship was linear over the range of CBF from 10 to 30 Hz (r2 = 0.83). At 37°C, CBF = 31+/-1 Hz, and TR = O.12+/-.02 mm/sec. equal to ovum transport rate in situ. The FLS is a valuable tool for characterizing ciliary activity and thus ovum transport function. Owing to the fact that ciliary dyskinesia resulting from disease of the fallopian tube is associated with infeitility, the FLS may be useful to acquire data important to the clinical evaluation of fallopian tube function and female infertility.

  1. 25-Gauge pars plana vitrectomy for ciliary block (malignant) glaucoma.

    Science.gov (United States)

    Meng, Li; Wei, Wenbin; Li, Yanlong; Hui, Xiaoying; Han, Xiaodong; Shi, Xiaobo

    2015-08-01

    To evaluate the efficacy of 25-ga pars plana vitrectomy (PPV) and 25-ga PPV combined with phacoemulsification surgery for managing ciliary block (malignant) glaucoma. The medical records of 11 eyes, in nine consecutive patients with malignant glaucoma were retrospectively reviewed to determine the efficacy of 25-ga PPV for alleviating this disease. All phakic eyes underwent phacoemulsification surgery during vitrectomy. Control of intraocular pressure (IOP), pre- and postoperative best-corrected visual acuity (BCVA), and development of intra- and postoperative complications were evaluated during follow-up. Malignant glaucoma was promptly resolved, and recurrence was not observed during a mean follow-up period of 12.7 ± 3.2 (range 6-18) months. The mean IOP decreased from 35.9 ± 2.9 mmHg, preoperatively, to 16.0 ± 1.4 mmHg, measured at last visit (P = 0.013). Although IOP control was achieved in all eyes after surgery, two eyes required long-term topical antiglaucoma medication. The mean logarithm of the minimum angle of resolution BCVA improved from +0.69 ± 0.11, preoperatively, to +0.29 ± 0.07, postoperatively (P = 0.027). No severe intraoperative complications were observed. Postoperative complications mostly occurred during the first week after surgery, including transient corneal edema and Descemet's membrane folds in three eyes, fibrotic exudation in 2, local iris posterior synechia in 1, and transient hypotony in 1, which were resolved spontaneously or with medication. The 25-ga PPV procedure seems to be safe and effective for treating malignant glaucoma. Combined vitrectomy with phacoemulsification surgery and a staged surgical approach may improve the success rate. PMID:25062710

  2. Ammonia excretion in mytilid mussels is facilitated by ciliary beating.

    Science.gov (United States)

    Thomsen, J; Himmerkus, N; Holland, N; Sartoris, F J; Bleich, M; Tresguerres, M

    2016-08-01

    The excretion of nitrogenous waste products in the form of ammonia (NH3) and ammonium (NH4 (+)) is a fundamental process in aquatic organisms. For mytilid bivalves, little is known about the mechanisms and sites of excretion. This study investigated the localization and the mechanisms of ammonia excretion in mytilid mussels. An Rh protein was found to be abundantly expressed in the apical cell membrane of the plicate organ, which was previously described as a solely respiratory organ. The Rh protein was also expressed in the gill, although at significantly lower concentrations, but was not detectable in mussel kidney. Furthermore, NH3/NH4 (+) was not enriched in the urine, suggesting that kidneys are not involved in active NH3/NH4 (+) excretion. Exposure to elevated seawater pH of 8.5 transiently reduced NH3/NH4 (+) excretion rates, but they returned to control values following 24 h acclimation. These mussels had increased abundance of V-type H(+)-ATPase in the apical membranes of plicate organ cells; however, NH3/NH4 (+) excretion rates were not affected by the V-type H(+)-ATPase specific inhibitor concanamycin A (100 nmol l(-1)). In contrast, inhibition of ciliary beating with dopamine and increased seawater viscosity significantly reduced NH3 excretion rates under control pH (8.0). These results suggest that NH3/NH4 (+) excretion in mytilid mussels takes place by passive NH3 diffusion across respiratory epithelia via the Rh protein, facilitated by the water current produced for filter feeding, which prevents accumulation of NH3 in the boundary layer. This mechanism would be energy efficient for sessile organisms, as they already generate water currents for filter feeding. PMID:27489216

  3. Quantitative optical coherence tomography imaging of intermediate flow defect phenotypes in ciliary physiology and pathophysiology

    Science.gov (United States)

    Huang, Brendan K.; Gamm, Ute A.; Jonas, Stephan; Khokha, Mustafa K.; Choma, Michael A.

    2015-03-01

    Cilia-driven fluid flow is a critical yet poorly understood aspect of pulmonary physiology. Here, we demonstrate that optical coherence tomography-based particle tracking velocimetry can be used to quantify subtle variability in cilia-driven flow performance in Xenopus, an important animal model of ciliary biology. Changes in flow performance were quantified in the setting of normal development, as well as in response to three types of perturbations: mechanical (increased fluid viscosity), pharmacological (disrupted serotonin signaling), and genetic (diminished ciliary motor protein expression). Of note, we demonstrate decreased flow secondary to gene knockdown of kif3a, a protein involved in ciliogenesis, as well as a dose-response decrease in flow secondary to knockdown of dnah9, an important ciliary motor protein.

  4. A Case of Incomplete Central Retinal Artery Occlusion Associated with Short Posterior Ciliary Artery Occlusion

    Directory of Open Access Journals (Sweden)

    Shinji Makino

    2013-01-01

    Full Text Available To our knowledge, incomplete central retinal artery occlusion associated with short posterior ciliary artery occlusion is extremely rare. Herein, we describe a case of a 62-year-old man who was referred to our hospital with of transient blindness in his right eye. At initial examination, the patient’s best-corrected visual acuity was 18/20 in the right eye. Fundus examination showed multiple soft exudates around the optic disc and mild macular retinal edema in his right eye; however, a cherry red spot on the macula was not detected. Fluorescein angiography revealed delayed dye inflow into the nasal choroidal hemisphere that is supplied by the short posterior ciliary artery. The following day, the patient’s visual acuity improved to 20/20. Soft exudates around the optic disc increased during observation and gradually disappeared. His hemodynamic parameters revealed subclavian steal syndrome as examined by cervical ultrasonography and digital subtraction angiography. We speculate that his transient blindness was due to ophthalmic artery spasms. In this particular case, spasms of the ophthalmic artery and occlusion of the short posterior ciliary artery occurred simultaneously. As the short posterior ciliary artery branches from the ophthalmic artery, the anatomical location of the lesion might be near the branching of both arteries.

  5. ZMYND10 is mutated in primary ciliary dyskinesia and interacts with LRRC6

    DEFF Research Database (Denmark)

    Zariwala, Maimoona A; Gee, Heon Yung; Kurkowiak, Małgorzata;

    2013-01-01

    Defects of motile cilia cause primary ciliary dyskinesia (PCD), characterized by recurrent respiratory infections and male infertility. Using whole-exome resequencing and high-throughput mutation analysis, we identified recessive biallelic mutations in ZMYND10 in 14 families and mutations in the...

  6. Choice of nasal nitric oxide technique as first-line test for primary ciliary dyskinesia

    DEFF Research Database (Denmark)

    Marthin, J K; Nielsen, K G

    2011-01-01

    Nasal nitric oxide (nNO) has a well-known potential as an indirect discriminative marker between patients with primary ciliary dyskinesia (PCD) and healthy subjects, but real-life experience and usefulness in young children is sparsely reported. Three nNO sampling methods were examined and compared...

  7. Growth of the crabgrass species Digitaria ciliaris and Digitaria nuda Crescimento das espécies de capim-colchão Digitaria ciliaris e Digitaria nuda

    Directory of Open Access Journals (Sweden)

    R.C. Souza

    2012-06-01

    Full Text Available The aim of this research paper was to compare the growth of D. ciliaris and D. nuda crabgrass species under non-competitive conditions. To this end, two experiments were conducted, one from March - July 2010 and the other from February - June 2011. The experimental design of both trials was completely randomized making a factorial (2 seasons x 2 species crabgrass x 12 evaluation periods with four replications. Assessments began at 15 days after sowing (DAS, and repeated weekly until 92 DAS. The variables evaluated were total dry matter (roots+leaves+stems, leaf area, leaf number and tiller. The results were submitted to analysis of variance and the absolute growth rate, relative growth rate and leaf area ratio were calculated using the means, which were adjusted regression models. The crabgrass species were significantly different in leaf area, leaf number, tiller number and dry matter per plant. D. ciliaris for all variables was statistically higher than D. nuda. Regarding the speed at which the growth of the species occurred, the absolute growth rate and relative growth rate of D. ciliaris was also greater than D. nuda. In addition, D. ciliaris also had a lower leaf area ratio indicating greater efficiency in converting light energy into carbohydrates. It can be concluded that D. ciliaris has a higher growth rate in conditions where there is no limitation of nutrients and water availability in relation to D. nuda, mainly due to D. ciliaris have greater leaf area, number of leaves and dry matter accumulation per plant.O objetivo da presente pesquisa foi comparar o crescimento das espécies de capim colchão D. ciliaris e D. nuda, em condições não-competitivas. Para isso, foram conduzidos dois experimentos, um de março a julho de 2010 e outro de fevereiro a junho de 2011. O delineamento experimental de ambos os ensaios foi inteiramente casualizado, perfazendo um esquema fatorial (2 épocas x 2 espécies de capim colchão x 12 períodos de

  8. CCDC65 mutation causes primary ciliary dyskinesia with normal ultrastructure and hyperkinetic cilia.

    Directory of Open Access Journals (Sweden)

    Amjad Horani

    Full Text Available BACKGROUND: Primary ciliary dyskinesia (PCD is a genetic disorder characterized by impaired ciliary function, leading to chronic sinopulmonary disease. The genetic causes of PCD are still evolving, while the diagnosis is often dependent on finding a ciliary ultrastructural abnormality and immotile cilia. Here we report a novel gene associated with PCD but without ciliary ultrastructural abnormalities evident by transmission electron microscopy, but with dyskinetic cilia beating. METHODS: Genetic linkage analysis was performed in a family with a PCD subject. Gene expression was studied in Chlamydomonas reinhardtii and human airway epithelial cells, using RNA assays and immunostaining. The phenotypic effects of candidate gene mutations were determined in primary culture human tracheobronchial epithelial cells transduced with gene targeted shRNA sequences. Video-microscopy was used to evaluate cilia motion. RESULTS: A single novel mutation in CCDC65, which created a termination codon at position 293, was identified in a subject with typical clinical features of PCD. CCDC65, an orthologue of the Chlamydomonas nexin-dynein regulatory complex protein DRC2, was localized to the cilia of normal nasal epithelial cells but was absent in those from the proband. CCDC65 expression was up-regulated during ciliogenesis in cultured airway epithelial cells, as was DRC2 in C. reinhardtii following deflagellation. Nasal epithelial cells from the affected individual and CCDC65-specific shRNA transduced normal airway epithelial cells had stiff and dyskinetic cilia beating patterns compared to control cells. Moreover, Gas8, a nexin-dynein regulatory complex component previously identified to associate with CCDC65, was absent in airway cells from the PCD subject and CCDC65-silenced cells. CONCLUSION: Mutation in CCDC65, a nexin-dynein regulatory complex member, resulted in a frameshift mutation and PCD. The affected individual had altered cilia beating patterns, and

  9. Ciliary beating recovery in deficient human airway epithelial cells after lentivirus ex vivo gene therapy.

    Directory of Open Access Journals (Sweden)

    Brigitte Chhin

    2009-03-01

    Full Text Available Primary Ciliary Dyskinesia is a heterogeneous genetic disease that is characterized by cilia dysfunction of the epithelial cells lining the respiratory tracts, resulting in recurrent respiratory tract infections. Despite lifelong physiological therapy and antibiotics, the lungs of affected patients are progressively destroyed, leading to respiratory insufficiency. Recessive mutations in Dynein Axonemal Intermediate chain type 1 (DNAI1 gene have been described in 10% of cases of Primary Ciliary Dyskinesia. Our goal was to restore normal ciliary beating in DNAI1-deficient human airway epithelial cells. A lentiviral vector based on Simian Immunodeficiency Virus pseudotyped with Vesicular Stomatitis Virus Glycoprotein was used to transduce cultured human airway epithelial cells with a cDNA of DNAI1 driven by the Elongation Factor 1 promoter. Transcription and translation of the transduced gene were tested by RT-PCR and western blot, respectively. Human airway epithelial cells that were DNAI1-deficient due to compound heterozygous mutations, and consequently had immotile cilia and no outer dynein arm, were transduced by the lentivirus. Cilia beating was recorded and electron microscopy of the cilia was performed. Transcription and translation of the transduced DNAI1 gene were detected in human cells treated with the lentivirus. In addition, immotile cilia recovered a normal beat and outer dynein arms reappeared. We demonstrated that it is possible to obtain a normalization of ciliary beat frequency of deficient human airway epithelial cells by using a lentivirus to transduce cells with the therapeutic gene. This preliminary step constitutes a conceptual proof that is indispensable in the perspective of Primary Ciliary Dyskinesia's in vivo gene therapy. This is the first time that recovery of cilia beating is demonstrated in this disease.

  10. Ciliary and mucus-net filter feeding, with special reference to fluid mechanical characteristics

    DEFF Research Database (Denmark)

    Jørgensen, C.B.; Kiørboe, Thomas; Møhlenberg, F.;

    1984-01-01

    Filter characteristics were determined and compared in ciliary and mucus-net filter feeders. The ciliary feeders include the polychaete Sabella penicillus, the brachiopod Terebratulina retusa, the marine bivalves Monia squama, Cardium glaucum, and Petricola pholadiformis, and the freshwater...... bivalves Dreissena polymorpha, Unio pictorum and Anodonta cygnea. The mucus-net feeders are the polychaete Chaetopterus variopedatus, the gastropod Crepidula fornicata and the ascidians Styela clava, Ciona intestinalis, Ascidia virginea, A. obliqua and A. mentula. Efficiencies of particle retention as a...... function of particle size was determined by counting of particles in samples of inhalant and exhalant water. The lower threshold for efficient particle retention varied from .apprx. 6 .mu.m in T. retuso to .apprx. 1 .mu.m in D. polymorpha. Mucus nets efficiently retained particles down to 1-2 .mu.m. Filter...

  11. Vortex arrays and ciliary tangles underlie the feeding-swimming tradeoff in starfish larvae

    CERN Document Server

    Gilpin, William; Prakash, Manu

    2016-01-01

    Many marine invertebrates have larval stages covered in linear arrays of beating cilia, which propel the animal while simultaneously entraining planktonic prey. These bands are strongly conserved across taxa spanning four major superphyla, and they are responsible for the unusual morphologies of many invertebrates. However, few studies have investigated their underlying hydrodynamics. Here, we study the ciliary bands of starfish larvae, and discover a beautiful pattern of slowly-evolving vortices that surrounds the swimming animals. Closer inspection of the bands reveals unusual ciliary "tangles" analogous to topological defects that break-up and re-form as the animal adjusts its swimming stroke. Quantitative experiments and modeling suggest that these vortices create a physical tradeoff between feeding and swimming, which manifests as distinct flow patterns or "eigenstrokes" representing each behavior---potentially implicating neuronal control of cilia. This quantitative interplay between larval form and hyd...

  12. Longitudinal study of lung function in a cohort of primary ciliary dyskinesia

    DEFF Research Database (Denmark)

    Ellerman, A; Bisgaard, H

    1997-01-01

    Patients with primary ciliary dyskinesia (PCD) have pronounced stasis of their respiratory secretions and therefore recurrent lower airway infections, which raises concerns for the development of lung function. Twenty four patients with PCD have been studied prospectively with a standardized regime...... in our clinic for 2-16 yrs with clinic visits, including spirometry 2-4 times per year, daily physiotherapy and monthly sputum cultures with subsequent specific antibiotic treatment. Lung function was significantly lower in the 12 PCD patients entering the cohort as adults when compared to the PCD......, respectively, the lung function remained stable in most patients. It is concluded that primary ciliary dyskinesia is accompanied by a progressive deterioration in lung function if undertreated, but lung function can be maintained with appropriate antibiotic treatment and regular physiotherapy. This emphasizes...

  13. Identification of Ciliary and Ciliopathy Genes in Caenorhabditis Elegans through Comparative Genomics

    OpenAIRE

    Chen, Nansheng; Mah, Allan; Oliver E Blacque; Chu, Jeffrey; Phgora, Kiran; Bakhoum, Mathieu W.; Newbury, C. Rebecca Hunt; Khattra, Jaswinder; Chan, Susanna; Efimenko, Evgheni; Johnsen, Robert; Phirke, Prasad; Swoboda, Peter; Marra, Marco; Moerman, Donald

    2006-01-01

    Background The recent availability of genome sequences of multiple related Caenorhabditis species has made it possible to identify, using comparative genomics, similarly transcribed genes in Caenorhabditis elegans and its sister species. Taking this approach, we have identified numerous novel ciliary genes in C. elegans, some of which may be orthologs of unidentified human ciliopathy genes. Results By screening for genes possessing canonical X-box sequences in promoters of three Caenorhabditi...

  14. LRRC6 mutation causes primary ciliary dyskinesia with dynein arm defects.

    Directory of Open Access Journals (Sweden)

    Amjad Horani

    Full Text Available Despite recent progress in defining the ciliome, the genetic basis for many cases of primary ciliary dyskinesia (PCD remains elusive. We evaluated five children from two unrelated, consanguineous Palestinian families who had PCD with typical clinical features, reduced nasal nitric oxide concentrations, and absent dynein arms. Linkage analyses revealed a single common homozygous region on chromosome 8 and one candidate was conserved in organisms with motile cilia. Sequencing revealed a single novel mutation in LRRC6 (Leucine-rich repeat containing protein 6 that fit the model of autosomal recessive genetic transmission, leading to a change of a highly conserved amino acid from aspartic acid to histidine (Asp146His. LRRC6 was localized to the cytoplasm and was up-regulated during ciliogenesis in human airway epithelial cells in a Foxj1-dependent fashion. Nasal epithelial cells isolated from affected individuals and shRNA-mediated silencing in human airway epithelial cells, showed reduced LRRC6 expression, absent dynein arms, and slowed cilia beat frequency. Dynein arm proteins were either absent or mislocalized to the cytoplasm in airway epithelial cells from a primary ciliary dyskinesia subject. These findings suggest that LRRC6 plays a role in dynein arm assembly or trafficking and when mutated leads to primary ciliary dyskinesia with laterality defects.

  15. Mouse models of polycystic kidney disease induced by defects of ciliary proteins

    Directory of Open Access Journals (Sweden)

    Je Yeong Ko

    2013-02-01

    Full Text Available Polycystic kidney disease (PKD is a common hereditarydisorder which is characterized by fluid-filled cysts in thekidney. Mutation in either PKD1, encoding polycystin-1 (PC1,or PKD2, encoding polycystin-2 (PC2, are causative genes ofPKD. Recent studies indicate that renal cilia, known asmechanosensors, detecting flow stimulation through renaltubules, have a critical function in maintaining homeostasis ofrenal epithelial cells. Because most proteins related to PKD arelocalized to renal cilia or have a function in ciliogenesis.PC1/PC2 heterodimer is localized to the cilia, playing a role incalcium channels. Also, disruptions of ciliary proteins, exceptfor PC1 and PC2, could be involved in the induction ofpolycystic kidney disease. Based on these findings, variousPKD mice models were produced to understand the roles ofprimary cilia defects in renal cyst formation. In this review, wewill describe the general role of cilia in renal epithelial cells,and the relationship between ciliary defects and PKD. We alsodiscuss mouse models of PKD related to ciliary defects basedon recent studies. [BMB Reports 2013; 46(2: 73-79

  16. Morphological, histochemical and immunohistochemical characterization of secretory production of the ciliary glands in the porcine eyelid

    Directory of Open Access Journals (Sweden)

    T Yasui

    2009-06-01

    Full Text Available In addition to performing general histology and cytology of the ciliary glands of the miniature pig, we studied the localization of glycoconjugates and b-defensins in these glands with the use of carbohydrate histochemical and immunohistochemical methods. The secretory cells of the glands were equipped with non-homogeneous secretory granules, a welldeveloped Golgi apparatus and rough endoplasmic reticulum. The secretory epithelium and luminal secretion of the glands contained large amounts of neutral and acidic glycoconjugates with various saccharide residues (a-L-Fuc, b-DGal, a-D-GalNAc and sialic acid. The sebaceous glands and tarsal glands also exhibited positive reactions to most of the histochemical methods. Additionally, the antimicrobial peptide group of b-defensins was demonstrated to be products of the ciliary glands, as well as the sebaceous glands and tarsal glands. The results obtained are discussed with regard to the specific function of the ciliary glandular secretions. These secretory products may be related to the moistening and general protection of the skin surface of the eyelid and ocular surface.

  17. Long-term clearance from small airways in subjects with ciliary dysfunction

    Directory of Open Access Journals (Sweden)

    Hjelte Lena

    2006-05-01

    Full Text Available Abstract The objective of this study was to investigate if long-term clearance from small airways is dependent on normal ciliary function. Six young adults with primary ciliary dyskinesia (PCD inhaled 111 Indium labelled Teflon particles of 4.2 μm geometric and 6.2 μm aerodynamic diameter with an extremely slow inhalation flow, 0.05 L/s. The inhalation method deposits particles mainly in the small conducting airways. Lung retention was measured immediately after inhalation and at four occasions up to 21 days after inhalation. Results were compared with data from ten healthy controls. For additional comparison three of the PCD subjects also inhaled the test particles with normal inhalation flow, 0.5 L/s, providing a more central deposition. The lung retention at 24 h in % of lung deposition (Ret24 was higher (p 24 with slow inhalation flow was 73.9 ± 1.9 % compared to 68.9 ± 7.5 % with normal inhalation flow in the three PCD subjects exposed twice. During day 7–21 the three PCD subjects exposed twice cleared 9 % with normal flow, probably representing predominantly alveolar clearance, compared to 19 % with slow inhalation flow, probably representing mainly small airway clearance. This study shows that despite ciliary dysfunction, clearance continues in the small airways beyond 24 h. There are apparently additional clearance mechanisms present in the small airways.

  18. Chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor promotes sciatic nerve repair.

    Science.gov (United States)

    Zhang, Yanru; Zhang, Hui; Katiella, Kaka; Huang, Wenhua

    2014-07-15

    A chemically extracted acellular allogeneic nerve graft can reduce postoperative immune rejection, similar to an autologous nerve graft, and can guide neural regeneration. However, it remains poorly understood whether a chemically extracted acellular allogeneic nerve graft combined with neurotrophic factors provides a good local environment for neural regeneration. This study investigated the repair of injured rat sciatic nerve using a chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor. An autologous nerve anastomosis group and a chemical acellular allogeneic nerve bridging group were prepared as controls. At 8 weeks after repair, sciatic functional index, evoked potential amplitude of the soleus muscle, triceps wet weight recovery rate, total number of myelinated nerve fibers and myelin sheath thickness were measured. For these indices, values in the three groups showed the autologous nerve anastomosis group > chemically extracted acellular nerve graft + ciliary neurotrophic factor group > chemical acellular allogeneic nerve bridging group. These results suggest that chemically extracted acellular nerve grafts combined with ciliary neurotrophic factor can repair sciatic nerve defects, and that this repair is inferior to autologous nerve anastomosis, but superior to chemically extracted acellular allogeneic nerve bridging alone. PMID:25221592

  19. Chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor promotes sciatic nerve repair

    Institute of Scientific and Technical Information of China (English)

    Yanru Zhang; Hui Zhang; Kaka Katiella; Wenhua Huang

    2014-01-01

    A chemically extracted acellular allogeneic nerve graft can reduce postoperative immune re-jection, similar to an autologous nerve graft, and can guide neural regeneration. However, it remains poorly understood whether a chemically extracted acellular allogeneic nerve graft combined with neurotrophic factors provides a good local environment for neural regenera-tion. This study investigated the repair of injured rat sciatic nerve using a chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor. An autologous nerve anastomosis group and a chemical acellular allogeneic nerve bridging group were prepared as controls. At 8 weeks after repair, sciatic functional index, evoked potential amplitude of the soleus muscle, triceps wet weight recovery rate, total number of myelinated nerve fibers and myelin sheath thickness were measured. For these indices, values in the three groups showed the autologous nerve anastomosis group > chemically extracted acellular nerve graft + ciliary neurotrophic factor group > chemical acellular allogeneic nerve bridging group. These results suggest that chemically extracted acellular nerve grafts combined with ciliary neurotrophic factor can repair sciatic nerve defects, and that this repair is inferior to autologous nerve anasto-mosis, but superior to chemically extracted acellular allogeneic nerve bridging alone.

  20. Ciliary muscle contraction force and trapezius muscle activity during manual tracking of a moving visual target.

    Science.gov (United States)

    Domkin, Dmitry; Forsman, Mikael; Richter, Hans O

    2016-06-01

    Previous studies have shown an association of visual demands during near work and increased activity of the trapezius muscle. Those studies were conducted under stationary postural conditions with fixed gaze and artificial visual load. The present study investigated the relationship between ciliary muscle contraction force and trapezius muscle activity across individuals during performance of a natural dynamic motor task under free gaze conditions. Participants (N=11) tracked a moving visual target with a digital pen on a computer screen. Tracking performance, eye refraction and trapezius muscle activity were continuously measured. Ciliary muscle contraction force was computed from eye accommodative response. There was a significant Pearson correlation between ciliary muscle contraction force and trapezius muscle activity on the tracking side (0.78, pmuscle contraction during continuous eye-hand coordination, may increase trapezius muscle tension and thus contribute to the development of musculoskeletal complaints in the neck-shoulder area. Further experimental studies are required to clarify whether the relationship is valid within each individual or may represent a general personal trait, when individuals with higher eye accommodative response tend to have higher trapezius muscle activity. PMID:26746010

  1. HEATR2 plays a conserved role in assembly of the ciliary motile apparatus.

    Directory of Open Access Journals (Sweden)

    Christine P Diggle

    2014-09-01

    Full Text Available Cilia are highly conserved microtubule-based structures that perform a variety of sensory and motility functions during development and adult homeostasis. In humans, defects specifically affecting motile cilia lead to chronic airway infections, infertility and laterality defects in the genetically heterogeneous disorder Primary Ciliary Dyskinesia (PCD. Using the comparatively simple Drosophila system, in which mechanosensory neurons possess modified motile cilia, we employed a recently elucidated cilia transcriptional RFX-FOX code to identify novel PCD candidate genes. Here, we report characterization of CG31320/HEATR2, which plays a conserved critical role in forming the axonemal dynein arms required for ciliary motility in both flies and humans. Inner and outer arm dyneins are absent from axonemes of CG31320 mutant flies and from PCD individuals with a novel splice-acceptor HEATR2 mutation. Functional conservation of closely arranged RFX-FOX binding sites upstream of HEATR2 orthologues may drive higher cytoplasmic expression of HEATR2 during early motile ciliogenesis. Immunoprecipitation reveals HEATR2 interacts with DNAI2, but not HSP70 or HSP90, distinguishing it from the client/chaperone functions described for other cytoplasmic proteins required for dynein arm assembly such as DNAAF1-4. These data implicate CG31320/HEATR2 in a growing intracellular pre-assembly and transport network that is necessary to deliver functional dynein machinery to the ciliary compartment for integration into the motile axoneme.

  2. Primary ciliary dyskinesia: a report from ATS 2001, May 18–23, San Francisco

    Directory of Open Access Journals (Sweden)

    Noone Peadar G

    2001-06-01

    Full Text Available Abstract Primary ciliary dyskinesia (PCD is a genetic disorder of abnormal ciliary structure and function that leads to defective mucociliary clearance, resulting in oto-sino-pulmonary disease, and infertility. The disease is currently under intense investigation by a number of research groups worldwide. At the recent American Thoracic Society meeting in San Francisco in May 2001, two sessions focused on PCD; a symposium session on May 21 with several featured expert speakers was followed by a mini-symposium on Tuesday May 22, with one featured speaker and presentation of nine abstracts covering a range of research topics. Mattias Salathe (University of Miami, USA and Stephen Brody (Washington University, St Louis, USA chaired the symposium session. Presentations focused on the clinical spectrum of PCD, the genetics of PCD, a proteomics approach to detail the structure of cilia, the role of cilia in the embryology of situs laterality, and airway epithelial cell biology. The mini-symposium was chaired by Peadar Noone (University of North Carolina, USA and Malcolm King (University of Alberta, USA and included presentations on the use of PCD as a human disease model, accurate definition of the phenotype using clinical and cell biologic markers, and molecular studies. The latter reports ranged from isolation of a protein involved in ciliary structure and function to genetic studies using linkage analysis and the candidate gene approach. Clinicians and scientists alike displayed considerable interest at both sessions, and there were several lively question–answer sessions.

  3. Ciliary metachronal wave propagation on the compliant surface of Paramecium cells.

    Science.gov (United States)

    Narematsu, Naoki; Quek, Raymond; Chiam, Keng-Hwee; Iwadate, Yoshiaki

    2015-12-01

    Ciliary movements in protozoa exhibit metachronal wave-like coordination, in which a constant phase difference is maintained between adjacent cilia. It is at present generally thought that metachronal waves require hydrodynamic coupling between adjacent cilia and the extracellular fluid. To test this hypothesis, we aspirated a Paramecium cell using a micropipette which completely sealed the surface of the cell such that no fluid could pass through the micropipette. Thus, the anterior and the posterior regions of the cell were hydrodynamically decoupled. Nevertheless, we still observed that metachronal waves continued to propagate from the anterior to the posterior ends of the cell, suggesting that in addition to hydrodynamic coupling, there are other mechanisms that can also transmit the metachronal waves. Such transmission was also observed in computational modeling where the fluid was fully decoupled between two partitions of a beating ciliary array. We also imposed cyclic stretching on the surface of live Paramecium cells and found that metachronal waves persisted in the presence of cyclic stretching. This demonstrated that, in addition to hydrodynamic coupling, a compliant substrate can also play a critical role in mediating the propagation of metachronal waves. PMID:26616106

  4. The Role of Ion Channels to Regulate Airway Ciliary Beat Frequency During Allergic Inflammation.

    Science.gov (United States)

    Joskova, M; Sutovska, M; Durdik, P; Koniar, D; Hargas, L; Banovcin, P; Hrianka, M; Khazaei, V; Pappova, L; Franova, S

    2016-01-01

    Overproduction of mucus is a hallmark of asthma. The aim of this study was to identify potentially effective therapies for removing excess mucus. The role of voltage-gated (Kir 6.1, KCa 1.1) and store-operated ion channels (SOC, CRAC) in respiratory cilia, relating to the tracheal ciliary beat frequency (CBF), was compared under the physiological and allergic airway conditions. Ex vivo experiments were designed to test the local effects of Kir 6.1, KCa 1.1 and CRAC ion channel modulators in a concentration-dependent manner on the CBF. Cilia, obtained with the brushing method, were monitored by a high-speed video camera and analyzed with ciliary analysis software. In natural conditions, a Kir 6.1 opener accelerated CBF, while CRAC blocker slowed it in a concentration-dependent manner. In allergic inflammation, the effect of Kir 6.1 opener was insignificant, with a tendency to decrease CBF. A cilio-inhibitory effect of a CRAC blocker, while gently reduced by allergic inflammation, remained significant. A KCa 1.1 opener turned out to significantly enhance the CBF under the allergic OVA-sensitized conditions. We conclude that optimally attuned concentration of KCa 1.1 openers or special types of bimodal SOC channel blockers, potentially given by inhalation, might benefit asthma. PMID:27369295

  5. Measurement of ciliary beat frequency using ultra-high resolution optical coherence tomography

    Science.gov (United States)

    Chen, Jason J.; Jing, Joseph C.; Su, Erica; Badger, Christopher; Coughlan, Carolyn A.; Chen, Zhongping; Wong, Brian J. F.

    2016-02-01

    Ciliated epithelial cells populate up to 80% of the surface area of the human airway and are responsible for mucociliary transport, which is the key protective mechanism that provides the first line of defense in the respiratory tract. Cilia beat in a rhythmic pattern and may be easily affected by allergens, pollutants, and pathogens, altering ciliary beat frequency (CBF) subsequently. Diseases including cystic fibrosis, chronic obstructive pulmonary disease, and primary ciliary dyskinesia may also decrease CBF. CBF is therefore a critical component of respiratory health. The current clinical method of measuring CBF is phase-contrast microscopy, which involves a tissue biopsy obtained via brushing of the nasal cavity. While this method is minimally invasive, the tissue sample must be oriented to display its profile view, making the visualization of a single layer of cilia challenging. In addition, the conventional method requires subjective analysis of CBF, e.g., manually counting by visual inspection. On the contrary, optical coherence tomography (OCT) has been used to study the retina in ophthalmology as well as vasculature in cardiology, and offers higher resolution than conventional computed tomography and magnetic resonance imaging. Based on this technology, our lab specifically developed an ultra-high resolution OCT system to image the microstructure of the ciliated epithelial cells. Doppler analysis was also performed to determine CBF. Lastly, we also developed a program that utilizes fast Fourier transform to determine CBF under phase-contrast microscopy, providing a more objective method compared to the current method.

  6. Effects of perfluorooctane sulfonate on tracheal ciliary beating frequency in mice

    International Nuclear Information System (INIS)

    Perfluorooctane sulfonate (PFOS) is one of the emerging persistent organic pollutants, ubiquitously found in the global environment, even in human serum. PFOS has been reported to perturb Ca2+ homeostasis in Paramecium, cardiomyocytes and neurons. Since ciliary beat frequency (CBF) in the trachea is known to be increased by cytoplasmic Ca2+ elevation, the effects of PFOS on CBF were evaluated in a slice preparation using video-enhanced contrast microscopy. PFOS increased CBF by 11% (P 2+ concentration ([Ca2+]i) in mouse tracheal ciliary cells. In Ca2+-free solution, PFOS at 100 μM failed to increase CBF (0.96-fold of vehicle control). The addition of Gd3+ (1 μM), a store-operated Ca2+ channel blocker, did not prevent the increase in CBF (1.09-fold (P + concentration (50 mM), which causes depolarization of the plasma membrane potential and a transient increase in [Ca2+]i, increased CBF by 20% (P 2+ channels (VDCCs) in stimulation of CBF. Nifedipine (30 μM), a selective VDCC blocker, antagonized the effects of high K+ (0.92-fold of high K+ solution) and PFOS (0.96-fold of vehicle control) on CBF. In cells from peroxisome proliferator-activated receptor α (PPARα)-null mice, PFOS still increased CBF (1.12-fold (P 2+ through VDCC

  7. Convergent evolution of RFX transcription factors and ciliary genes predated the origin of metazoans

    Directory of Open Access Journals (Sweden)

    Chen Nansheng

    2010-05-01

    Full Text Available Abstract Background Intraflagellar transport (IFT genes, which are critical for the development and function of cilia and flagella in metazoans, are tightly regulated by the Regulatory Factor X (RFX transcription factors (TFs. However, how and when their evolutionary relationship was established remains unknown. Results We have identified evidence suggesting that RFX TFs and IFT genes evolved independently and their evolution converged before the first appearance of metazoans. Both ciliary genes and RFX TFs exist in all metazoans as well as some unicellular eukaryotes. However, while RFX TFs and IFT genes are found simultaneously in all sequenced metazoan genomes, RFX TFs do not co-exist with IFT genes in most pre-metazoans and thus do not regulate them in these organisms. For example, neither the budding yeast nor the fission yeast possesses cilia although both have well-defined RFX TFs. Conversely, most unicellular eukaryotes, including the green alga Chlamydomonas reinhardtii, have typical cilia and well conserved IFT genes but lack RFX TFs. Outside of metazoans, RFX TFs and IFT genes co-exist only in choanoflagellates including M. brevicollis, and only one fungus Allomyces macrogynus of the 51 sequenced fungus genomes. M. brevicollis has two putative RFX genes and a full complement of ciliary genes. Conclusions The evolution of RFX TFs and IFT genes were independent in pre-metazoans. We propose that their convergence in evolution, or the acquired transcriptional regulation of IFT genes by RFX TFs, played a pivotal role in the establishment of metazoan.

  8. Chemical constituents of Cenchrus ciliaris L. from the Cholistan desert, Pakistan

    Directory of Open Access Journals (Sweden)

    Ashraf Muhammad Aqeel

    2013-01-01

    Full Text Available The Cholistan Desert is an extension of the Great Indian Desert, covering an area of 26,330 km2. The desert can be divided into two main geomorphic regions: the northern region, known as Lesser Cholistan, constituting the desert margin and consisting of a series of saline alluvial flats alternating with low sand ridges/dunes; and the southern region, known as Greater Cholistan, a wind-resorted sandy desert comprised of a number of old Hakra River terraces with various forms of sand ridges and inter-ridge valleys. Cholistan Desert presents a complex pattern of alluvial and aeolian depositions. In the present study we evaluated the nutritive value of different accessions of the perennial range grass Cenchrus ciliaris collected from the Cholistan Desert, Pakistan. Standard method, Benedict’s quantitative reagent for carbohydrates, crude protein and nitrogen by the Kjeldahl method, mineral analysis by flame photometer and estimation of crude fiber by using acid base treatment, were utilized. The results suggest that Cenchrus ciliaris has medicinal and nutritional importance, and that it could be a good source of important nutrients for humans, helping to alleviate poverty in poor local communities.

  9. C2 Domains as Protein-Protein Interaction Modules in the Ciliary Transition Zone

    Directory of Open Access Journals (Sweden)

    Kim Remans

    2014-07-01

    Full Text Available RPGR-interacting protein 1 (RPGRIP1 is mutated in the eye disease Leber congenital amaurosis (LCA and its structural homolog, RPGRIP1-like (RPGRIP1L, is mutated in many different ciliopathies. Both are multidomain proteins that are predicted to interact with retinitis pigmentosa G-protein regulator (RPGR. RPGR is mutated in X-linked retinitis pigmentosa and is located in photoreceptors and primary cilia. We solved the crystal structure of the complex between the RPGR-interacting domain (RID of RPGRIP1 and RPGR and demonstrate that RPGRIP1L binds to RPGR similarly. RPGRIP1 binding to RPGR affects the interaction with PDEδ, the cargo shuttling factor for prenylated ciliary proteins. RPGRIP1-RID is a C2 domain with a canonical β sandwich structure that does not bind Ca2+ and/or phospholipids and thus constitutes a unique type of protein-protein interaction module. Judging from the large number of C2 domains in most of the ciliary transition zone proteins identified thus far, the structure presented here seems to constitute a cilia-specific module that is present in multiprotein transition zone complexes.

  10. Posterior Ciliary Artery Occlusion Caused by Hyaluronic Acid Injections Into the Forehead

    Science.gov (United States)

    Hu, Xiu Zhuo; Hu, Jun Yan; Wu, Peng Sen; Yu, Sheng Bo; Kikkawa, Don O.; Lu, Wei

    2016-01-01

    Abstract Although cosmetic facial soft tissue fillers are generally safe and effective, improper injections can lead to devastating and irreversible consequences. We represent the first known case of posterior ciliary artery occlusion caused by hyaluronic acid. A 41-year-old female presented with right visual loss 7 hours after receiving cosmetic hyaluronic acid injections into her forehead. Examination revealed no light perception in the right eye and multiple dark ischemic area of injection over the forehead and nose. The right fundus revealed a pink retina with optic nerve edema. Fluorescein angiogram showed several filling defects in the choroidal circulation and late hyperfluorescence in the choroid. A right posterior ciliary artery occlusion and embolic occlusion of facial artery braches was diagnosed. With hyaluronidase injection, hyperbaric oxygen therapy, oral aspirin, oral acetazolamide and dexamethasone venotransfuse treatment, the patient's forehead and nasal skin improved and vision recovered to hand movements. With proper technique, vascular occlusion is rare following facial filler injection. Vision consequences can be severe if filler emboli enter the ocular circulation. Physicians should be aware of this potential side effect, recognize its presentation, and be knowledgeable of effective management. PMID:26986163

  11. Posterior Ciliary Artery Occlusion Caused by Hyaluronic Acid Injections Into the Forehead: A Case Report.

    Science.gov (United States)

    Hu, Xiu Zhuo; Hu, Jun Yan; Wu, Peng Sen; Yu, Sheng Bo; Kikkawa, Don O; Lu, Wei

    2016-03-01

    Although cosmetic facial soft tissue fillers are generally safe and effective, improper injections can lead to devastating and irreversible consequences. We represent the first known case of posterior ciliary artery occlusion caused by hyaluronic acid. A 41-year-old female presented with right visual loss 7 hours after receiving cosmetic hyaluronic acid injections into her forehead. Examination revealed no light perception in the right eye and multiple dark ischemic area of injection over the forehead and nose. The right fundus revealed a pink retina with optic nerve edema. Fluorescein angiogram showed several filling defects in the choroidal circulation and late hyperfluorescence in the choroid. A right posterior ciliary artery occlusion and embolic occlusion of facial artery braches was diagnosed. With hyaluronidase injection, hyperbaric oxygen therapy, oral aspirin, oral acetazolamide and dexamethasone venotransfuse treatment, the patient's forehead and nasal skin improved and vision recovered to hand movements. With proper technique, vascular occlusion is rare following facial filler injection. Vision consequences can be severe if filler emboli enter the ocular circulation. Physicians should be aware of this potential side effect, recognize its presentation, and be knowledgeable of effective management. PMID:26986163

  12. [A case of primary ciliary dyskinesia who had been treated as asthma].

    Science.gov (United States)

    Hosoki, Koa; Fujisawa, Takao; Masuda, Sawako; Usui, Satoko; Ito, Hiroaki; Nagao, Mizuho; Terada, Akihiko; Iguchi, Kousei; Ogawa, Satoru; Nakatani, Kaname; Takeuchi, Kazuhiko

    2010-07-01

    We report a case of 18-old girl with primary ciliary dyskinesia (PCD) who had been diagnosed as asthma. Since birth, she had presented with unexplained productive cough, sputum, rhinorrhea, and stridor with situs solitus. Her familial history was negative for PCD. At 2 years of age, ciliary beat frequency and beat pattern were normal. She was diagnosed as rhinosinusitis, chronic secretory otitis media. At 3 years of age, she was diagnosed as asthma because of wheezing not associated with respiratory infection. Various asthma medications were then administered, including DSCG, inhaled corticosteroids, and salmeterol, but varying responses to the treatment were noted. Spirometry revealed persistent severe small airway obstruction. Beta2 agonist reversibility was recognized by impulse oscillation system, not with FEV1. At age of 18, chest CT disclosed bronchiectasis, and nasal nitric oxide concentration was very low, 98 ppb and a diagnostic approach for PCD was performed. Electron microscopic analysis of nasal cilia demonstrated defects of the outer and inner dynein arms, and the diagnosis of PCD was made. Mutations in DNAH1 and DNAI1 genes were found. The diagnosis of PCD is often difficult in the absence of situs inversus totalis. Recurrent wheeze with chronic rhinosinusitis, chronic otitis media, and brochiectasis may warrant detailed investigations for PCD, especially with nasal NO measurement. PMID:20703071

  13. Fetal calf serum-mediated inhibition of neurite growth from ciliary ganglion neurons in vitro.

    Science.gov (United States)

    Davis, G E; Skaper, S D; Manthorpe, M; Moonen, G; Varon, S

    1984-01-01

    Embryonic chick ciliary ganglion (CG) neurons cultured in fetal calf serum-containing medium have been previously reported to extend neurites on polyornithine (PORN) substrata precoated with a neurite-promoting factor (PNPF) from rat schwannoma-conditioned medium. On PORN substrata alone, however, no neuritic growth occurred. This was interpreted as evidence that PORN was an incompetent substratum for ciliary neuritic growth. In this study, we now find that an untreated PORN substratum allows neuritic growth in serum-free defined medium. When PNPF was added to PORN, a more rapid and extensive neuritic response occurred. After 5 hr of culture, a 60% neuritic response occurred on PNPF/PORN, whereas no neurons initiated neurites until 10-12 hr on PORN. The inhibitory effect of fetal calf serum noted above on PORN could be obtained in part by pretreating the substratum with serum for 1 hr. Maximal inhibitory effects in the PORN pretreatment were achieved after 30 min and were not further improved by treatments up to 4 hr. Bovine serum albumin was also found to inhibit neurite growth on PORN to about 60% of the inhibition obtained by an equivalent amount of serum protein. Fetal calf serum was shown to cause a 15% reduction in the percentage of neurons bearing neurites after its addition to 18-hr serum-free PORN cultures and to cause statistically significant reductions in neurite lengths measured 2 hr later.

  14. Genotypic variation in response to salinity in a new sexual germplasm of Cenchrus ciliaris L.

    Science.gov (United States)

    Quiroga, Mariana; Tommasino, Exequiel; Griffa, Sabrina; Ribotta, Andrea; Colomba, Eliana López; Carloni, Edgardo; Grunberg, Karina

    2016-05-01

    As part of a breeding program for new salt-tolerant sexual genotypes of Cenchrus ciliaris L., here we evaluated the salt-stress response of two new sexual hybrids, obtained by controlled crosses, at seedling and germination stages. A seedling hydroponic experiment with 300 mM NaCl was performed and physiological variables and growth components were evaluated. While salt-treated sexual material did not show a decrease in productivity with respect to control plants, a differential response in some physiological characteristics was observed. Sexual hybrid 1-9-1 did not suffer oxidative damage and its proline content did not differ from that of control treatment. By contrast, sexual hybrid 1-7-11 suffered oxidative damage and accumulated proline, maintaining its growth under saline stress. At the germination stage, sexual hybrid 1-9-1 presented the highest Germination Rate Index at the maximum NaCl concentration assayed, suggesting an ecological advantage in this genotype. These new sexual resources are promising maternal parental with differential response to salt and could be incorporated in a breeding program of C. ciliaris in the search of new genotypes tolerant to salinity. PMID:26906150

  15. Presence of collagen IV in the ciliary zonules of the human eye : An immunohistochemical study by LM and TEM

    NARCIS (Netherlands)

    Los, LI; van der Worp, RJ; van Luyn, MJA; Hooymans, JMM

    2004-01-01

    The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission

  16. Continued administration of ciliary neurotrophic factor protects mice from inflammatory pathology in experimental autoimmune encephalomyelitis

    DEFF Research Database (Denmark)

    Kuhlmann, Tanja; Remington, Leah; Cognet, Isabelle;

    2006-01-01

    Multiple sclerosis is an inflammatory disease of the central nervous system that leads to loss of myelin and oligodendrocytes and damage to axons. We show that daily administration (days 8 to 24) of murine ciliary neurotrophic factor (CNTF), a neurotrophic factor that has been described...... as a survival and differentiation factor for neurons and oligodendrocytes, significantly ameliorates the clinical course of a mouse model of multiple sclerosis. In the acute phase of experimental autoimmune encephalomyelitis induced by myelin oligodendrocyte glycoprotein peptide 35-55, treatment with CNTF did...... it was withdrawn. After cessation of CNTF treatment, inflammation and symptoms returned to control levels. However, slight but significantly higher numbers of oligodendrocytes, NG2-positive cells, axons, and neurons were observed in mice that had been treated with high concentrations of CNTF. Our results show...

  17. The presence of synaptic and chromosome disjunction mutants in Cenchrus ciliaris (Poaceae: Paniceae

    Directory of Open Access Journals (Sweden)

    N. C. Visser

    1999-12-01

    Full Text Available Synaptic mutants are present in  Cenchrus ciliaris L This species, due to the presence of linear bivalents and occasion­al trivalents and quadrivalents, is an intermediate desynaptic species. In addition, geographical distribution and environmental factors, such as high temperatures and low humidity, could also have had an influence on the desynapsis observed.The disjunction of chromosomes during anaphase I was mostly abnormal in this desynaptic species. Precocious disjunction of chromosomes into chromatids occurred during anaphase I Due to the high incidence of this chromosome abnormality, a mutant gene,  'pc'  responsible for the disjunction of chromosomes, must be present. The absence of cytokinesis in one specimen indicates a recessive mutant gene,  'va' to be active in this species.

  18. Efficiency optimization and symmetry-breaking in a model of ciliary locomotion

    CERN Document Server

    Michelin, Sebastien

    2010-01-01

    A variety of swimming microorganisms, called ciliates, exploit the bending of a large number of small and densely-packed organelles, termed cilia, in order to propel themselves in a viscous fluid. We consider a spherical envelope model for such ciliary locomotion where the dynamics of the individual cilia are replaced by that of a continuous overlaying surface allowed to deform tangentially to itself. Employing a variational approach, we determine numerically the time-periodic deformation of such surface which leads to low-Reynolds locomotion with minimum rate of energy dissipation (maximum efficiency). Employing both Lagrangian and Eulerian points of views, we show that in the optimal swimming stroke, individual cilia display weak asymmetric beating, but that a significant symmetry-breaking occurs at the organism level, with the whole surface deforming in a wave-like fashion reminiscent of metachronal waves of biological cilia. This wave motion is analyzed using a formal modal decomposition, is found to occu...

  19. Effects of ouabain and furosemide on transepithelial electrical parameters of the isolated shark ciliary epithelium.

    Science.gov (United States)

    Wiederholt, M; Zadunaisky, J A

    1987-08-01

    Sections of the ciliary epithelium of adult sharks (Squalus acanthias) were mounted in Ussing-type chambers (area 0.2 cm2) for measurements of transepithelial potential difference (PD), short circuit current (SCC) and calculation of transepithelial resistance (R). In 15 preparations PD was aqueous side negative (-0.51 +/- 0.12 mV; SCC 18.3 +/- 2.5 microA cm-2; R 30.7 +/- 3.1 Ohm cm2). However, in 15 other preparations incubated in identical Ringer's solution PD was aqueous side positive (0.53 +/- 0.09 mV; SCC -19.6 +/- 2.3 microA cm-2; R 27.9 +/- 2.8 Ohm cm2). 10(-5) M ouabain or 10(-4) M furosemide were applied either to the aqueous or blood side of the isolated ciliary epithelium at transepithelial negative or positive PD. When the transepithelial PD was positive on the aqueous side ouabain decreased PD and SCC within 15 to 45 min. When the spontaneous PD was negative both PD and SCC decreased when ouabain was applied to the blood side. When the drug was given to the aqueous side a biphasic response (first stimulation, then inhibition) of PD and SCC was observed. Furosemide when given to the blood side (with aqueous side PD positive) or to the aqueous side (with aqueous side PD negative) decreased PD and SCC. However, a transient stimulation of both electrical parameters was observed when furosemide was applied to either the blood side (with aqueous side PD negative) or to the aqueous side (with aqueous side PD positive). The polarity and magnitude of PD and SCC probably depend on the relative activity of sodium and chloride pumps across the cell membranes of the non-pigmented and/or pigmented cell layer. However, additional transport mechanisms cannot be excluded. PMID:3038770

  20. DNAH6 and Its Interactions with PCD Genes in Heterotaxy and Primary Ciliary Dyskinesia

    Science.gov (United States)

    Onuoha, Ezenwa Obi; Damerla, Rama Rao; Francis, Richard; Furutani, Yoshiyuki; Tariq, Muhammad; King, Stephen M.; Hendricks, Gregory; Cui, Cheng; Saydmohammed, Manush; Lee, Dong Min; Zahid, Maliha; Sami, Iman; Leatherbury, Linda; Pazour, Gregory J.; Ware, Stephanie M.; Nakanishi, Toshio; Goldmuntz, Elizabeth; Tsang, Michael; Lo, Cecilia W.

    2016-01-01

    Heterotaxy, a birth defect involving left-right patterning defects, and primary ciliary dyskinesia (PCD), a sinopulmonary disease with dyskinetic/immotile cilia in the airway are seemingly disparate diseases. However, they have an overlapping genetic etiology involving mutations in cilia genes, a reflection of the common requirement for motile cilia in left-right patterning and airway clearance. While PCD is a monogenic recessive disorder, heterotaxy has a more complex, largely non-monogenic etiology. In this study, we show mutations in the novel dynein gene DNAH6 can cause heterotaxy and ciliary dysfunction similar to PCD. We provide the first evidence that trans-heterozygous interactions between DNAH6 and other PCD genes potentially can cause heterotaxy. DNAH6 was initially identified as a candidate heterotaxy/PCD gene by filtering exome-sequencing data from 25 heterotaxy patients stratified by whether they have airway motile cilia defects. dnah6 morpholino knockdown in zebrafish disrupted motile cilia in Kupffer’s vesicle required for left-right patterning and caused heterotaxy with abnormal cardiac/gut looping. Similarly DNAH6 shRNA knockdown disrupted motile cilia in human and mouse respiratory epithelia. Notably a heterotaxy patient harboring heterozygous DNAH6 mutation was identified to also carry a rare heterozygous PCD-causing DNAI1 mutation, suggesting a DNAH6/DNAI1 trans-heterozygous interaction. Furthermore, sequencing of 149 additional heterotaxy patients showed 5 of 6 patients with heterozygous DNAH6 mutations also had heterozygous mutations in DNAH5 or other PCD genes. We functionally assayed for DNAH6/DNAH5 and DNAH6/DNAI1 trans-heterozygous interactions using subthreshold double-morpholino knockdown in zebrafish and showed this caused heterotaxy. Similarly, subthreshold siRNA knockdown of Dnah6 in heterozygous Dnah5 or Dnai1 mutant mouse respiratory epithelia disrupted motile cilia function. Together, these findings support an oligogenic disease

  1. DNAH6 and Its Interactions with PCD Genes in Heterotaxy and Primary Ciliary Dyskinesia.

    Directory of Open Access Journals (Sweden)

    You Li

    2016-02-01

    Full Text Available Heterotaxy, a birth defect involving left-right patterning defects, and primary ciliary dyskinesia (PCD, a sinopulmonary disease with dyskinetic/immotile cilia in the airway are seemingly disparate diseases. However, they have an overlapping genetic etiology involving mutations in cilia genes, a reflection of the common requirement for motile cilia in left-right patterning and airway clearance. While PCD is a monogenic recessive disorder, heterotaxy has a more complex, largely non-monogenic etiology. In this study, we show mutations in the novel dynein gene DNAH6 can cause heterotaxy and ciliary dysfunction similar to PCD. We provide the first evidence that trans-heterozygous interactions between DNAH6 and other PCD genes potentially can cause heterotaxy. DNAH6 was initially identified as a candidate heterotaxy/PCD gene by filtering exome-sequencing data from 25 heterotaxy patients stratified by whether they have airway motile cilia defects. dnah6 morpholino knockdown in zebrafish disrupted motile cilia in Kupffer's vesicle required for left-right patterning and caused heterotaxy with abnormal cardiac/gut looping. Similarly DNAH6 shRNA knockdown disrupted motile cilia in human and mouse respiratory epithelia. Notably a heterotaxy patient harboring heterozygous DNAH6 mutation was identified to also carry a rare heterozygous PCD-causing DNAI1 mutation, suggesting a DNAH6/DNAI1 trans-heterozygous interaction. Furthermore, sequencing of 149 additional heterotaxy patients showed 5 of 6 patients with heterozygous DNAH6 mutations also had heterozygous mutations in DNAH5 or other PCD genes. We functionally assayed for DNAH6/DNAH5 and DNAH6/DNAI1 trans-heterozygous interactions using subthreshold double-morpholino knockdown in zebrafish and showed this caused heterotaxy. Similarly, subthreshold siRNA knockdown of Dnah6 in heterozygous Dnah5 or Dnai1 mutant mouse respiratory epithelia disrupted motile cilia function. Together, these findings support an

  2. Differences between the neurogenic and proliferative abilities of Müller glia with stem cell characteristics and the ciliary epithelium from the adult human eye

    OpenAIRE

    Bhatia, Bhairavi; Jayaram, Hari; Singhal, Shweta; Jones, Megan F; Limb, G. Astrid

    2011-01-01

    Much controversy has arisen on the nature and sources of stem cells in the adult human retina. Whilst ciliary epithelium has been thought to constitute a source of neural stem cells, a population of Müller glia in the neural retina has also been shown to exhibit neurogenic characteristics. This study aimed to compare the neurogenic and proliferative abilities between these two major cell populations. It also examined whether differences exist between the pigmented and non-pigmented ciliary ep...

  3. CCDC151 mutations cause primary ciliary dyskinesia by disruption of the outer dynein arm docking complex formation

    DEFF Research Database (Denmark)

    Hjeij, Rim; Onoufriadis, Alexandros; Watson, Christopher M;

    2014-01-01

    disorder of ciliary and flagellar dysmotility characterized by chronic upper and lower respiratory infections and defects in laterality. Here, by combined high-throughput mapping and sequencing, we identified CCDC151 loss-of-function mutations in five affected individuals from three independent families...... whose cilia showed a complete loss of ODAs and severely impaired ciliary beating. Consistent with the laterality defects observed in these individuals, we found Ccdc151 expressed in vertebrate left-right organizers. Homozygous zebrafish ccdc151(ts272a) and mouse Ccdc151(Snbl) mutants display a spectrum...... of situs defects associated with complex heart defects. We demonstrate that CCDC151 encodes an axonemal coiled coil protein, mutations in which abolish assembly of CCDC151 into respiratory cilia and cause a failure in axonemal assembly of the ODA component DNAH5 and the ODA-DC-associated components CCDC114...

  4. Clinical value of measurement of pulmonary radioaerosol mucociliary clearance in the work up of primary ciliary dyskinesia

    DEFF Research Database (Denmark)

    Munkholm, Mathias; Nielsen, Kim Gjerum; Mortensen, Jann

    2015-01-01

    BACKGROUND: We aimed to evaluate and define the general clinical applicability and impact of pulmonary radioaerosol mucociliary clearance (PRMC) on the work up of patients suspected of having primary ciliary dyskinesia (PCD). In addition, we wanted to evaluate the accuracy of the reference values...... primarily to results from nasal ciliary function testing, to electron microscopic (EM) examination of the ultrastructure of the cilia, and to the final clinical diagnosis. RESULTS: Of the 239 patients, 27 ended up with a final clinical diagnosis of definitive PCD. No patients with a PRMC test...... that was normal or otherwise not consistent with PCD ended up with PCD as final clinical diagnosis (though a minority of patients in this group ended up unresolved in regard to PCD). Forty percent of patients with an abnormal PRMC test ended up with PCD as final clinical diagnosis. Furthermore, the PRMC test had...

  5. RPGR-containing protein complexes in syndromic and non-syndromic retinal degeneration due to ciliary dysfunction

    Indian Academy of Sciences (India)

    Carlos A. Murga-Zamalloa; Anand Swaroop; Hemant Khanna

    2009-12-01

    Dysfunction of primary cilia due to mutations in cilia-centrosomal proteins is associated with pleiotropic disorders. The primary (or sensory) cilium of photoreceptors mediates polarized trafficking of proteins for efficient phototransduction. Retinitis pigmentosa GTPase regulator (RPGR) is a cilia-centrosomal protein mutated in >70% of X-linked RP cases and 10%–20% of simplex RP males. Accumulating evidence indicates that RPGR may facilitate the orchestration of multiple ciliary protein complexes. Disruption of these complexes due to mutations in component proteins is an underlying cause of associated photoreceptor degeneration. Here, we highlight the recent developments in understanding the mechanism of cilia-dependent photoreceptor degeneration due to mutations in RPGR and RPGR-interacting proteins in severe genetic diseases, including retinitis pigmentosa, Leber congenital amaurosis (LCA), Joubert syndrome, and Senior–Loken syndrome, and explore the physiological relevance of photoreceptor ciliary protein complexes.

  6. COMPARATIVE PRIMARY PHYTO-PROFILE AND MICROCIDAL ACTIVITY OF CENCHRUS CILIARIS (ANJAN GRASS AND WITHANIA SOMNIFERA (WINTER CHERRY

    Directory of Open Access Journals (Sweden)

    Singariya P.

    2012-04-01

    Full Text Available Crude extracts of different parts (root, stem, leaf and seed of Cenchrus ciliaris (CAZRI-358 and (root, stem, leaf and flower of Withania somnifera (RUBL-20668 and were successively extracted with polar to non polar solvents (water, chloroform and benzene using soxhlet assembly. The extracts were then screened for their antimicrobial activity in-vitro against one gram positive bacteria (Bacillus subtilis, two gram negative bacteria (Pseudomonas aeruginosa and Enterobactor aerogens and one fungus (Aspergillus flavus by disc diffusion assay. Serial dilution method was used to determine minimum inhibitory concentration (MIC and minimum bactericidal/fungicidal concentration (MBC/MFC. Chloroform extract of leaves of both the plants showed highest activity, by W. somnifera (IZ-20.83±0.21 mm, AI- 1.389 and (IZ-20.67±0.24 mm, AI- 1.148 by C. ciliaris against B. subtilis and P. aeruginosa respectively.

  7. Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy

    OpenAIRE

    Čelakovský Pavel; Stejskal Lubomír; Raška Otakar; Klusáková Ilona; Dubový Petr; Haninec Pavel

    2011-01-01

    Abstract Background It is difficult to repair nerve if proximal stump is unavailable or autogenous nerve grafts are insufficient for reconstructing extensive nerve damage. Therefore, alternative methods have been developed, including lateral anastomosis based on axons' ability to send out collateral sprouts into denervated nerve. The different capacity of a sensory or motor axon to send a sprout is controversial and may be controlled by cytokines and/or neurotrophic factors like ciliary neuro...

  8. Rer1p maintains ciliary length and signaling by regulating γ-secretase activity and Foxj1a levels.

    Science.gov (United States)

    Jurisch-Yaksi, Nathalie; Rose, Applonia J; Lu, Huiqi; Raemaekers, Tim; Munck, Sebastian; Baatsen, Pieter; Baert, Veerle; Vermeire, Wendy; Scales, Suzie J; Verleyen, Daphne; Vandepoel, Roel; Tylzanowski, Przemko; Yaksi, Emre; de Ravel, Thomy; Yost, H Joseph; Froyen, Guy; Arrington, Cammon B; Annaert, Wim

    2013-03-18

    Cilia project from the surface of most vertebrate cells and are important for several physiological and developmental processes. Ciliary defects are linked to a variety of human diseases, named ciliopathies, underscoring the importance of understanding signaling pathways involved in cilia formation and maintenance. In this paper, we identified Rer1p as the first endoplasmic reticulum/cis-Golgi-localized membrane protein involved in ciliogenesis. Rer1p, a protein quality control receptor, was highly expressed in zebrafish ciliated organs and regulated ciliary structure and function. Both in zebrafish and mammalian cells, loss of Rer1p resulted in the shortening of cilium and impairment of its motile or sensory function, which was reflected by hearing, vision, and left-right asymmetry defects as well as decreased Hedgehog signaling. We further demonstrate that Rer1p depletion reduced ciliary length and function by increasing γ-secretase complex assembly and activity and, consequently, enhancing Notch signaling as well as reducing Foxj1a expression.

  9. Sonic Hedgehog dependent phosphorylation by CK1α and GRK2 is required for ciliary accumulation and activation of smoothened.

    Directory of Open Access Journals (Sweden)

    Yongbin Chen

    2011-06-01

    Full Text Available Hedgehog (Hh signaling regulates embryonic development and adult tissue homeostasis through the GPCR-like protein Smoothened (Smo, but how vertebrate Smo is activated remains poorly understood. In Drosophila, Hh dependent phosphorylation activates Smo. Whether this is also the case in vertebrates is unclear, owing to the marked sequence divergence between vertebrate and Drosophila Smo (dSmo and the involvement of primary cilia in vertebrate Hh signaling. Here we demonstrate that mammalian Smo (mSmo is activated through multi-site phosphorylation of its carboxyl-terminal tail by CK1α and GRK2. Phosphorylation of mSmo induces its active conformation and simultaneously promotes its ciliary accumulation. We demonstrate that graded Hh signals induce increasing levels of mSmo phosphorylation that fine-tune its ciliary localization, conformation, and activity. We show that mSmo phosphorylation is induced by its agonists and oncogenic mutations but is blocked by its antagonist cyclopamine, and efficient mSmo phosphorylation depends on the kinesin-II ciliary motor. Furthermore, we provide evidence that Hh signaling recruits CK1α to initiate mSmo phosphorylation, and phosphorylation further increases the binding of CK1α and GRK2 to mSmo, forming a positive feedback loop that amplifies and/or sustains mSmo phosphorylation. Hence, despite divergence in their primary sequences and their subcellular trafficking, mSmo and dSmo employ analogous mechanisms for their activation.

  10. OFD1, as a Ciliary Protein, Exhibits Neuroprotective Function in Photoreceptor Degeneration Models.

    Directory of Open Access Journals (Sweden)

    Juan Wang

    Full Text Available Ofd1 is a newly identified causative gene for Retinitis pigmentosa (RP, a photoreceptor degenerative disease. This study aimed to examine Ofd1 localization in retina and further to investigate its function in photoreceptor degeneration models. Ofd1 localization in rat retina was examined using immunofluorescence. N-methyl-N-nitrosourea (MNU-induced rats and Royal College of Surgeons (RCS rats were used as photoreceptor degeneration models. The expression pattern of Ofd1, other ciliary associated genes and Wnt signaling pathway genes were examined in rat models. Furthermore, pEGFP-Ofd1-CDS and pSUPER-Ofd1-shRNA were constructed to overexpress and knockdown the expression level in 661W and R28 cells. MNU was also used to induce cell death. Cilia formation was observed using immunocytochemistry (ICC. Reactive oxygen species (ROS were detected using the 2', 7'-Dichlorofluorescin diacetate (DCFH-DA assay. Apoptosis genes expression was examined using qRT-PCR, Western blotting and fluorescence-activated cell sorting (FACS. Ofd1 localized to outer segments of rat retina photoreceptors. Ofd1 and other ciliary proteins expression levels increased from the 1st and 4th postnatal weeks and decreased until the 6th week in the RCS rats, while their expression consistently decreased from the 1st and 7th day in the MNU rats. Moreover, Wnt signaling pathway proteins expression was significantly up-regulated in both rat models. Knockdown of Ofd1 expression resulted in a smaller population, shorter length of cell cilia, and lower cell viability. Ofd1 overexpression partially attenuated MNU toxic effects by reducing ROS levels and mitigating apoptosis. To the best of our knowledge, this is the first study demonstrating Ofd1 localization and its function in rat retina and in retinal degeneration rat models. Ofd1 plays a role in controlling photoreceptor cilium length and number. Importantly, it demonstrates a neuroprotective function by protecting the photoreceptor

  11. Metastasis of Ciliary Body Melanoma to the Contralateral Eye: A Case Report and Review of Uveal Melanoma Literature

    Directory of Open Access Journals (Sweden)

    Nouritza M. Torossian

    2015-01-01

    Full Text Available Many types of cancers metastasize to the eye. However, uveal melanoma metastasizing to the contralateral choroid is very rare. We report the case of a 68-year-old man with history of treated uveal melanoma of the right eye that developed metastasis to the liver and the choroid of the left eye. Ten years earlier, he was diagnosed to have uveal melanoma of the right eye and was initially treated with plaque radiotherapy. Two years later, upon progression of the disease in the right eye he had enucleation of the eye. We describe the patient’s clinical history, the diagnosis of recurrent disease in the contralateral eye, therapy of the left eye, and systemic metastasis. In addition, we reviewed the published medical literature and described the recent advances in the management of uveal melanoma.

  12. Recent invasion of buffel grass (Cenchrus ciliaris of a natural protected area from the southern Sonoran Desert Invasión reciente de zacate buffel (Cenchrus ciliaris en un área natural protegida del desierto sonorense

    Directory of Open Access Journals (Sweden)

    Erick De la Barrera

    2008-12-01

    Full Text Available The Centro Ecológico de Sonora is a natural protected area where the natural vegetation remained undisturbed at least until 1997. Since then, Cenchrus ciliaris has become a prominent element of the vegetation because of disturbance. Climate, soil properties, population structure and biological activity for C. ciliaris were studied to gain understanding of the ecological mechanisms that favored the invasion by this exotic grass. Mean air temperature and annual rainfall were 24.8°C and 302 mm. The soil was a loamy-sand that was poor in most nutrients, but particularly rich in phosphorus. Pennisetum ciliare was the most abundant species at the Centro Ecológico, representing over one third of total plant ground cover. Basal area for individual plants ranged from less than 1 cm² to almost 1 m². Living leaves per plant increased with precipitation, peaking at 199 leaves in March 2005, and no living leaves were found after 103 days without rain. The environmental conditions prevalent at Centro Ecológico are very favorable for C. ciliaris, whose establishment was apparently triggered by a major disturbance caused by the development of housing projects.El Centro Ecológico de Sonora es un área natural protegida donde la vegetación autóctona permaneció sin disturbios por lo menos hasta 1997. Desde entonces, Cenchrus ciliaris se ha convertido en un elemento prominente de la vegetación. Se estudiaron el clima, las propiedades del suelo, la estructura de la población y la actividad biológica de C. ciliaris, como una aproximación al entendimiento de los mecanismos ecológicos que favorecieron la invasión por este pasto exótico. La temperatura media del aire y la precipitación anual fueron de 24.8 °C y 302 mm. El suelo fue una arena limosa pobre en minerales, pero particularmente rica en fósforo. Cenchrus ciliaris fue la especie herbácea más abundante en el Centro Ecológico, representando más de un tercio de la cobertura vegetal. El

  13. Analysis of ependymal ciliary beat pattern and beat frequency using high speed imaging: comparison with the photomultiplier and photodiode methods

    Directory of Open Access Journals (Sweden)

    O'Callaghan Chris

    2012-06-01

    Full Text Available Abstract Background The aim of this study was to compare beat frequency measurements of ependymal cilia made by digital high speed imaging to those obtained using the photomultiplier and modified photodiode techniques. Using high speed video analysis the relationship of the power and recover strokes was also determined. Methods Ciliated strips of ependyma attached to slices from the brain of Wistar rats were incubated at 30°C and observed using a ×50 water immersion lens. Ciliary beat frequency was measured using each of the three techniques: the high speed video, photodiode and photomultiplier. Readings were repeated after 30 minutes incubation at 37°C. Ependymal cilia were observed in slow motion and the precise movement of cilia during the recovery stroke relative to the path travelled during the power stroke was measured. Results The mean (95% confidence intervals beat frequencies determined by the high speed video, photomultiplier and photodiode at 30°C were 27.7 (26.6 to 28.8, 25.5 (24.4 to 26.6 and 20.8 (20.4 to 21.3 Hz, respectively. The mean (95% confidence intervals beat frequencies determined by the high speed video, photomultiplier and photodiode at 37°C were 36.4 (34 to 39.5, 38.4 (36.8 to 39.9 and 18.8 (16.9 to 20.5 Hz. The inter and intra observer reliability for measurement of ciliary beat frequency was 3.8% and 1%, respectively. Ependymal cilia were observed to move in a planar fashion during the power and recovery strokes with a maximum deviation to the right of the midline of 12.1(11.8 to 13.0° during the power stroke and 12.6(11.6 to 13.6° to the left of the midline during the recovery stroke. Conclusion The photodiode technique greatly underestimates ciliary beat frequency and should not be used to measure ependymal ciliary beat frequency at the temperatures studied. Ciliary beat frequency from the high speed video and photomultiplier techniques cannot be used interchangeably. Ependymal cilia had minimal deviation to

  14. MNS1 is essential for spermiogenesis and motile ciliary functions in mice.

    Directory of Open Access Journals (Sweden)

    Jian Zhou

    Full Text Available During spermiogenesis, haploid round spermatids undergo dramatic cell differentiation and morphogenesis to give rise to mature spermatozoa for fertilization, including nuclear elongation, chromatin remodeling, acrosome formation, and development of flagella. The molecular mechanisms underlining these fundamental processes remain poorly understood. Here, we report that MNS1, a coiled-coil protein of unknown function, is essential for spermiogenesis. We find that MNS1 is expressed in the germ cells in the testes and localizes to sperm flagella in a detergent-resistant manner, indicating that it is an integral component of flagella. MNS1-deficient males are sterile, as they exhibit a sharp reduction in sperm production and the remnant sperm are immotile with abnormal short tails. In MNS1-deficient sperm flagella, the characteristic arrangement of "9+2" microtubules and outer dense fibers are completely disrupted. In addition, MNS1-deficient mice display situs inversus and hydrocephalus. MNS1-deficient tracheal motile cilia lack some outer dynein arms in the axoneme. Moreover, MNS1 monomers interact with each other and are able to form polymers in cultured somatic cells. These results demonstrate that MNS1 is essential for spermiogenesis, the assembly of sperm flagella, and motile ciliary functions.

  15. Expression of ciliary neurotrophic factor after induction of ocular hypertension in the retina of rats

    Institute of Scientific and Technical Information of China (English)

    WU Qiang; ZHANG Min; SONG Bei-wen; LU Bin; HU Ping

    2007-01-01

    Background Glaucoma is mainly characterized by the loss of retinal ganglion cells. Ciliary neurotrophic factor (CNTF) is believed to stimulate the regeneration of axons of retinal ganglion cells. The objective of our study was to detect the expression of CNTF in the retina of a rat glaucoma model with increased intraocular pressure (lOP).Methods The rat glaucoma model was set up by electrocoagulating at least three episcleral and limbal veins. The location and the expression level of CNTF were detected at 1, 3, 7, 14, and 28 days post-surgery by immunohistochemistry, semiquantitative reverse-transcription polymerase chain reaction (RT-PCR), and Western blot analysis.Results The rat glaucoma model with chronic, moderately elevated lOP was successfully produced. A minimum expression of CNTF was found in the ganglion cell layer of the retinas of the control group, and temporally increased expression and intensity of CNTF were found in the experimental retinas.Conclusion The expression of endogenous CNTF in the rat retina was found altered after the induction of ocular hypertension.

  16. Peripheral sensory processing in mammalian gravity receptors - Observations of ciliary tuft configurations

    Science.gov (United States)

    Ross, Muriel D.; Donovan, Kathleen; Rogers, Charles

    1987-01-01

    Scanning electron microscopy was used to study dynamic polarizations of clustered cells of the anterior part of rat saccular macula and to shed light on the possible roles of two types of hair cells integrated into the same neural circuitry: those with short stereocilia and long kinocilium (ss/lk), and those with long stereocilia and still longer kinocilium (ls/lk). It was found that the ss/lk-type cells could be further subdivided into two types, whereas the ls/lk cells consisted of four major kinds. It was also found that the kinocilium was most often fixed in a recovery stroke position (curved basally, and the upper portion projected back over the tuft) and that the kinocilia were not aligned in parallel in any given part of a macula, even though each cilium pointed in the proper direction relative to the striola line. The possibility of a relationship between the ciliary tuft morphology and the function of the hair cell of which it is a part is discussed.

  17. Circulating levels of ciliary neurotrophic factor in normal pregnancy and preeclampsia

    Directory of Open Access Journals (Sweden)

    Akahori,Yoichiro

    2010-04-01

    Full Text Available

    Ciliary neurotrophic factor (CNTF has been shown to decrease food intake in mouse models of obesity and to improve insulin sensitivity. It is well known that tight regulation of glucose metabolism is essential for successful gestational outcomes (e.g. fetal growth, and that abnormal insulin resistance is associated with preeclampsia (PE. To investigate the possibility that CNTF might be involved in the regulation of insulin resistance during pregnancy, circulating levels of CNTF were assessed in non-pregnant, normal pregnant, postpartum, and pregnant women with PE. Sera from healthy non-pregnant women (n10, pregnant women (n30:1st trimester;n10, 2nd trimester n10;3rd trimester;n10, postpartum women (n10, and patients with PE (n11 were studied with Western blotting. Circulating CNTF was detected by Western blotting, and the levels of CNTF in pregnant women were decreased as compared with those in non-pregnant women, and tended to decrease as pregnancy progressed. A significant decrease was found in PE as compared with normal pregnancy. Circulating CNTF might be associated with physiological and abnormal insulin resistance during pregnancy.

  18. Preservation and Observation of Amterior Ciliary Vessels in Rectus Muscles during Strabismus Surgery

    Institute of Scientific and Technical Information of China (English)

    GuanghuanMai; JianhuaYan

    1995-01-01

    Purpose:To observe the form and number of the anterior ciliary vessels(ACV)in rectus muscles.To train technician in preservation an d obsevation of ACV,so preservation can be done in caes with a rish of anterior segment ischemia(ASI).Methods:Curved foreign knife,iris hook,plastic rubber band and standard operat-ing loupes(3x)or microscope were used in the surgery on 34cases of comitant strabismus and 18cases of paralytic strabismus.Results:The ACVs per muscle in medial,lateral,superior,inferior rectus were3.08,3.26,3.50and3.50respectively in 89muscles of 52surgical strabismus cas-es.All ACVs in 16rectus muscles and 90out of 220ACVs in 73rectus muscles were too small ortoo short to be dissected.The sucess rate of ACV preservation was91.5%(119/130).105out of 130vessels were saved using loupe magnifica-tion and 14pit pf130vessels were saved under operating microscope.Conclusions:The number of ACVinrectus muscles are more than 2in our obser-vation cases.The ACVpreservation has the clinical value of allowing us toper-form muscle sugery on three or more rectus muscles simultaneously and get fonal surgical results more earlier after acv in each muscle are dissected and preserved otherwinse staged surgery are needed.The each strabismus surgeon must knov this technique.

  19. Mutations in DNAJB13, Encoding an HSP40 Family Member, Cause Primary Ciliary Dyskinesia and Male Infertility.

    Science.gov (United States)

    El Khouri, Elma; Thomas, Lucie; Jeanson, Ludovic; Bequignon, Emilie; Vallette, Benoit; Duquesnoy, Philippe; Montantin, Guy; Copin, Bruno; Dastot-Le Moal, Florence; Blanchon, Sylvain; Papon, Jean François; Lorès, Patrick; Yuan, Li; Collot, Nathalie; Tissier, Sylvie; Faucon, Catherine; Gacon, Gérard; Patrat, Catherine; Wolf, Jean Philippe; Dulioust, Emmanuel; Crestani, Bruno; Escudier, Estelle; Coste, André; Legendre, Marie; Touré, Aminata; Amselem, Serge

    2016-08-01

    Primary ciliary dyskinesia (PCD) is an autosomal-recessive disease due to functional or ultra-structural defects of motile cilia. Affected individuals display recurrent respiratory-tract infections; most males are infertile as a result of sperm flagellar dysfunction. The great majority of the PCD-associated genes identified so far encode either components of dynein arms (DAs), which are multiprotein-ATPase complexes essential for ciliary motility, or proteins involved in DA assembly. To identify the molecular basis of a PCD phenotype characterized by central complex (CC) defects but normal DA structure, a phenotype found in ∼15% of cases, we performed whole-exome sequencing in a male individual with PCD and unexplained CC defects. This analysis, combined with whole-genome SNP genotyping, identified a homozygous mutation in DNAJB13 (c.833T>G), a gene encoding a HSP40 co-chaperone whose ortholog in the flagellated alga Chlamydomonas localizes to the radial spokes. In vitro studies showed that this missense substitution (p.Met278Arg), which involves a highly conserved residue of several HSP40 family members, leads to protein instability and triggers proteasomal degradation, a result confirmed by the absence of endogenous DNAJB13 in cilia and sperm from this individual. Subsequent DNAJB13 analyses identified another homozygous mutation in a second family; the study of DNAJB13 transcripts obtained from airway cells showed that this mutation (c.68+1G>C) results in a splicing defect consistent with a loss-of-function mutation. Overall, this study, which establishes mutations in DNAJB13 as a cause of PCD, unveils the key role played by DNAJB13 in the proper formation and function of ciliary and flagellar axonemes in humans. PMID:27486783

  20. The ciliary proteins Meckelin and Jouberin are required for retinoic acid-dependent neural differentiation of mouse embryonic stem cells.

    Science.gov (United States)

    Romani, Sveva; Illi, Barbara; De Mori, Roberta; Savino, Mauro; Gleeson, Joseph G; Valente, Enza Maria

    2014-01-01

    The dysfunction of the primary cilium, a complex, evolutionarily conserved, organelle playing an important role in sensing and transducing cell signals, is the unifying pathogenetic mechanism of a growing number of diseases collectively termed "ciliopathies", typically characterized by multiorgan involvement. Developmental defects of the central nervous system (CNS) characterize a subset of ciliopathies showing clinical and genetic overlap, such as Joubert syndrome (JS) and Meckel syndrome (MS). Although several knock-out mice lacking a variety of ciliary proteins have shown the importance of primary cilia in the development of the brain and CNS-derived structures, developmental in vitro studies, extremely useful to unravel the role of primary cilia along the course of neural differentiation, are still missing. Mouse embryonic stem cells (mESCs) have been recently proven to mimic brain development, giving the unique opportunity to dissect the CNS differentiation process along its sequential steps. In the present study we show that mESCs express the ciliary proteins Meckelin and Jouberin in a developmentally-regulated manner, and that these proteins co-localize with acetylated tubulin labeled cilia located at the outer embryonic layer. Further, mESCs differentiating along the neuronal lineage activate the cilia-dependent sonic hedgehog signaling machinery, which is impaired in Meckelin knock-out cells but results unaffected in Jouberin-deficient mESCs. However, both lose the ability to acquire a neuronal phenotype. Altogether, these results demonstrate a pivotal role of Meckelin and Jouberin during embryonic neural specification and indicate mESCs as a suitable tool to investigate the developmental impact of ciliary proteins dysfunction.

  1. Immersed boundary-lattice Boltzmann method for simulation of muco-ciliary transport: effect of mucus depth at various amounts of cilia beat frequency

    Science.gov (United States)

    Shahmardan, M. M.; Sedaghat, M. H.; Norouzi, M.; Nazari, M.

    2015-12-01

    Numerical simulation based on immersed boundary-lattice Boltzmann method has been employed to study 2D muco-ciliary transport problem. The periciliary liquid (PCL) and mucus layers in this study are considered as the Newtonian and viscoelastic fluid respectively. An Oldroyd-B model is used as the constitutive equations of mucus layer. To simulate accurate effects of the cilia and PCL-mucus interface on the fluid, immersed boundary method is used. Numerical simulations have been performed to investigate the effects of mucus depth on the muco-ciliary clearance at various values of cilia beat frequencies. Our results show that, by increasing mucus depth, which results from air pollution and smoking, mean mucus velocity decreases. But it can be completely modified by increasing cilia beat frequency and the cilia beat frequency has great effect on the muco-ciliary clearance.

  2. CCDC39 is required for assembly of inner dynein arms and the dynein regulatory complex and for normal ciliary motility in humans and dogs

    DEFF Research Database (Denmark)

    Merveille, Anne-Christine; Davis, Erica E; Becker-Heck, Anita;

    2011-01-01

    -assembly factors of axonemal dyneins, but these mutations account for only about 50% of cases of PCD. We exploited the unique properties of dog populations to positionally clone a new PCD gene, CCDC39. We found that loss-of-function mutations in the human ortholog underlie a substantial fraction of PCD cases...... with axonemal disorganization and abnormal ciliary beating. Functional analyses indicated that CCDC39 localizes to ciliary axonemes and is essential for assembly of inner dynein arms and the dynein regulatory complex....

  3. A case report of primary ciliary dyskinesia, laterality defects and developmental delay caused by the co-existence of a single gene and chromosome disorder.

    LENUS (Irish Health Repository)

    Casey, Jillian P

    2015-01-01

    Primary ciliary dyskinesia (PCD) is a rare autosomal recessive disorder characterised by abnormal ciliary motion and impaired mucociliary clearance, leading to recurrent respiratory infections, sinusitis, otitis media and male infertility. Some patients also have laterality defects. We recently reported the identification of three disease-causing PCD genes in the Irish Traveller population; RSPH4A, DYX1C1 and CCNO. We have since assessed an additional Irish Traveller family with a complex phenotype involving PCD who did not have any of the previously identified PCD mutations.

  4. [Endoscopically controlled optimization of trans-scleral suture fixation of posterior chamber lenses in the ciliary sulcus].

    Science.gov (United States)

    Althaus, C; Sundmacher, R

    1993-08-01

    Two technical difficulties have to be overcome in transscleral suture fixation of posterior chamber intraocular lenses (PCL) in the ciliary sulcus: first, exact needle penetration through the sulcus, and second, exact positioning of the PCL haptics in the sulcus. Incongruence of the two may lead to long-term complications by compression or even strangulation of ciliary processes. Intraocular endoscopy was used intraoperatively to visualize the site of needle penetration and the final location of the haptics in patients. It turned out that with our previously described standard techniques the precision was far less than anticipated. Thus, new technical ways had to be sought to improve the precision of positioning. In secondary implantation without perforating keratoplasty we achieved the best results when the needle was passed ab externo before opening the eye and before anterior vitrectomy, taking advantage of a precisely prepared sclerocorneal zone. Passing the needle ab externo in an already hypotonic eyeball gives much less precise results. In combination with perforating keratoplasty with an open-sky approach, needle penetration ab interno is reliable. Correct positioning of the PCL haptics is at least as difficult as correct needle penetration, a fact which up to now has mostly been ignored. In 33 consecutively operated eyes the technique of implantation and PCL design was varied under endoscopical control.(ABSTRACT TRUNCATED AT 250 WORDS)

  5. Strain-dependent brain defects in mouse models of primary ciliary dyskinesia with mutations in Pcdp1 and Spef2.

    Science.gov (United States)

    Finn, R; Evans, C C; Lee, L

    2014-09-26

    Hydrocephalus is caused by the accumulation of cerebrospinal fluid (CSF) in the cerebral ventricular system which results in an enlargement of the cranium due to increased intraventricular pressure. The increase in pressure within the brain typically results in sloughing of ciliated ependymal cells, loss of cortical gray matter, and increased gliosis. Congenital hydrocephalus is associated with several syndromes including primary ciliary dyskinesia (PCD), a rare, genetically heterogeneous, pediatric syndrome that results from defects in motile cilia and flagella. We have examined the morphological and physiological defects in the brains of two mouse models of PCD, nm1054 and bgh, which have mutations in Pcdp1 (also known as Cfap221) and Spef2, respectively. Histopathological and immunohistochemical analyses of mice with these mutations on the C57BL/6J and 129S6/SvEvTac genetic backgrounds demonstrate strain-dependent morphological brain damage. Alterations in astrocytosis, microglial activation, myelination, and the neuronal population were identified and are generally more severe on the C57BL/6J background. Analysis of ependymal ciliary clearance ex vivo and CSF flow in vivo demonstrate a physiological defect in nm1054 and bgh mice on both genetic backgrounds, indicating that abnormal cilia-driven flow is not the sole determinant of the severity of hydrocephalus in these models. These results suggest that genetic modifiers play an important role in susceptibility to severe PCD-associated hydrocephalus. PMID:25073043

  6. Caenorhabditis elegans ciliary protein NPHP-8, the homologue of human RPGRIP1L, is required for ciliogenesis and chemosensation.

    Science.gov (United States)

    Liu, Lin; Zhang, Mingshu; Xia, Zhiping; Xu, Pingyong; Chen, Liangyi; Xu, Tao

    2011-07-01

    Nephronophthisis (NPHP) is the most frequent genetic cause of end-stage renal failure in children and young adults. NPHP8/RPGRIP1L is a novel ciliary gene that, when mutated, in addition to causing NPHP, also causes Joubert syndrome (JBTS) and Meckel syndrome (MKS). The exact function of NPHP8 and how defects in NPHP8 lead to human diseases are poorly understood. Here, we studied the Caenorhabditis elegans homolog nphp-8 (C09G5.8) and explored the possible function of NPHP-8 in ciliated sensory neurons. We determined the gene structure of nphp-8 through rapid amplification of cDNA ends (RACE) analysis and discovered an X-box motif that had been previously overlooked. Moreover, NPHP-8 co-localized with NPHP-4 at the transition zone at the base of cilia. Mutation of nphp-8 led to abnormal dye filling (Dyf) and shorter cilia lengths in a subset of ciliary neurons. In addition, chemotaxis to several volatile attractants was significantly impaired in nphp-8 mutants. Our data suggest that NPHP-8/RPGRIP1L plays an important role in cilia formation and cilia-mediated chemosensation in a cell type-specific manner. PMID:21689635

  7. Action of Administered Ciliary Neurotrophic Factor on the Mouse Dorsal Vagal Complex

    Science.gov (United States)

    Senzacqua, Martina; Severi, Ilenia; Perugini, Jessica; Acciarini, Samantha; Cinti, Saverio; Giordano, Antonio

    2016-01-01

    Ciliary neurotrophic factor (CNTF) induces weight loss in obese rodents and humans through activation of the hypothalamic Jak-STAT (Janus kinase-signal transducer and activator of transcription) signaling pathway. Here, we tested the hypothesis that CNTF also affects the brainstem centers involved in feeding and energy balance regulation. To this end, wild-type and leptin-deficient (ob/ob and db/db) obese mice were acutely treated with intraperitoneal recombinant CNTF. Coronal brainstem sections were processed for immunohistochemical detection of STAT3, STAT1, STAT5 phosphorylation and c-Fos. In wild-type mice, CNTF treatment for 45 min induced STAT3, STAT1, and STAT5 phosphorylation in neurons as well as glial cells of the area postrema; here, the majority of CNTF-responsive cells activated multiple STAT isoforms, and a significant proportion of CNTF-responsive glial cells bore the immaturity and plasticity markers nestin and vimentin. After 120 min CNTF treatment, c-Fos expression was intense in glial cells and weak in neurons of the area postrema, it was intense in several neurons of the rostral and caudal solitary tract nucleus (NTS), and weak in some cholinergic neurons of the dorsal motor nucleus of the vagus. In the ob/ob and db/db mice, Jak-STAT activation and c-Fos expression were similar to those induced in wild-type mouse brainstem. Treatment with CNTF (120 min, to induce c-Fos expression) and leptin (25 min, to induce STAT3 phosphorylation) demonstrated the co-localization of the two transcription factors in a small neuron population in the caudal NTS portion. Finally, weak immunohistochemical CNTF staining, detected in funiculus separans, and meningeal glial cells, matched the modest amount of CNTF found by RT-qPCR in micropunched area postrema tissue, which in contrast exhibited a very high amount of CNTF receptor. Collectively, the present findings show that the area postrema and the NTS exhibit high, distinctive responsiveness to circulating

  8. Action of Administered Ciliary Neurotrophic Factor on the Mouse Dorsal Vagal Complex.

    Science.gov (United States)

    Senzacqua, Martina; Severi, Ilenia; Perugini, Jessica; Acciarini, Samantha; Cinti, Saverio; Giordano, Antonio

    2016-01-01

    Ciliary neurotrophic factor (CNTF) induces weight loss in obese rodents and humans through activation of the hypothalamic Jak-STAT (Janus kinase-signal transducer and activator of transcription) signaling pathway. Here, we tested the hypothesis that CNTF also affects the brainstem centers involved in feeding and energy balance regulation. To this end, wild-type and leptin-deficient (ob/ob and db/db) obese mice were acutely treated with intraperitoneal recombinant CNTF. Coronal brainstem sections were processed for immunohistochemical detection of STAT3, STAT1, STAT5 phosphorylation and c-Fos. In wild-type mice, CNTF treatment for 45 min induced STAT3, STAT1, and STAT5 phosphorylation in neurons as well as glial cells of the area postrema; here, the majority of CNTF-responsive cells activated multiple STAT isoforms, and a significant proportion of CNTF-responsive glial cells bore the immaturity and plasticity markers nestin and vimentin. After 120 min CNTF treatment, c-Fos expression was intense in glial cells and weak in neurons of the area postrema, it was intense in several neurons of the rostral and caudal solitary tract nucleus (NTS), and weak in some cholinergic neurons of the dorsal motor nucleus of the vagus. In the ob/ob and db/db mice, Jak-STAT activation and c-Fos expression were similar to those induced in wild-type mouse brainstem. Treatment with CNTF (120 min, to induce c-Fos expression) and leptin (25 min, to induce STAT3 phosphorylation) demonstrated the co-localization of the two transcription factors in a small neuron population in the caudal NTS portion. Finally, weak immunohistochemical CNTF staining, detected in funiculus separans, and meningeal glial cells, matched the modest amount of CNTF found by RT-qPCR in micropunched area postrema tissue, which in contrast exhibited a very high amount of CNTF receptor. Collectively, the present findings show that the area postrema and the NTS exhibit high, distinctive responsiveness to circulating

  9. A conserved role for Notch in priming the cellular response to Shh through ciliary localisation of the key Shh transducer, Smoothened

    DEFF Research Database (Denmark)

    Stasiulewicz, Magdalena; Gray, Shona; Mastromina, Ioanna;

    2015-01-01

    , we show Notch activity promotes longer primary cilia both in vitro and in vivo. Strikingly, these Notch-regulated effects are Shh-independent. These data identify Notch signalling as a novel modulator of Shh signalling which acts mechanistically via regulation of ciliary localisation of key...

  10. Mutations in ZMYND10, a gene essential for proper axonemal assembly of inner and outer dynein arms in humans and flies, cause primary ciliary dyskinesia

    DEFF Research Database (Denmark)

    Moore, Daniel J; Onoufriadis, Alexandros; Shoemark, Amelia;

    2013-01-01

    Primary ciliary dyskinesia (PCD) is a ciliopathy characterized by airway disease, infertility, and laterality defects, often caused by dual loss of the inner dynein arms (IDAs) and outer dynein arms (ODAs), which power cilia and flagella beating. Using whole-exome and candidate-gene Sanger resequ...

  11. Accuracy and repeatability of direct ciliary sulcus diameter measurements by full-scale 50-megahertz ultrasound biomicroscopy

    Institute of Scientific and Technical Information of China (English)

    LI De-jiao; WANG Ning-li; CHEN Shu; LI Shu-ning; MU Da-peng; WANG Tao

    2009-01-01

    Background Phakic intraocular lens (pIOL) implantation has been a popular means for the treatment of high ametropia. Measurements of ciliary sulcus diameter is important for pIOL size determining. But till now, no perfect system can directly measure it. The present study was to evaluate the accuracy, repeatability and reproducibility of direct sulcus diameter measurements obtained by a full-scale 50-megahertz (MHz) ultrasound biomicroscopy (UBM).Methods A fresh cadaver human eye with a scale marker inserted through the posterior chamber plane from 3 o'clock to 9 o'clock meridian and 30 randomly selected eyes from 30 normal subjects were scanned by full-scale 50-MHz UBM in horizontal meridional scan plane. The distance between the scales and the whole length of the marker inside the cadaver eye were measured by the same observer using the "built-in" measurement tools and the indicating error of instrument was calculated. Reproducibility of the measurement was evaluated in 30 eyes by 2 operators using Blander and Altman plot test. Repeatability was evaluated from 10 successive eyes randomly selected from the 30 eyes by one operator.Results On a scale of 1 mm, the greatest indicating error was 40 μm; the mean largest indicating error of 1 mm scale from the 10 images was (26±14) μm; on a scale of 11 mm, the greatest indicating error was 70 μo; the error rate was 0.64%. The mean length of the needle inside the eye of the 10 images was 11.05 mm, with the mean indicating error of 47 μm, the average error rate was 0.43%. For ciliary sulcus diameter measurements in vivo, the coefficient of variation was 0.38%; the coefficients of repeatability for intra-observer and inter-observer measurements were 1.99% and 2.55%, respectively. The limits of agreement for intra-observer and inter-observer measurement were-0.41 mm to 0.48 mm and -0.59 mm to 0.58 ram, respectively.Conclusion The full-scale 50-MHz UBM can be a high accuracy and good repeatability means for direct

  12. Forage supply in thinned Caatinga enriched with buffel grass (Cenchrus ciliaris L. grazed by goats and sheep=Oferta de forragem em Caatinga raleada e enriquecida com capim buffel (Cenchrus ciliaris L. pastejada por ovinos e caprinos

    Directory of Open Access Journals (Sweden)

    Diogo da Costa Soares

    2012-04-01

    Full Text Available Forage supply from herbs was assessed in a thinned Caatinga enriched with buffel grass (Cenchrus ciliaris L. exposed to goat and sheep grazing. The 2.4 ha experimental area, located at the Experimental Station of the Federal University of Campina Grande, in Santa Terezinha, Paraíba State, Brazil, was divided into four 0.6 ha paddocks, which were further subdivided into two 0.3 ha experimental plots. Twelve F1 (Boer x SRD goats and 12 Santa Inês sheep were divided in four groups of six animals of the same species. The herbaceous vegetation was separated into buffel grass (Cenchrus ciliaris L., dicotyledons and other grass species. Treatments were randomized to plots according to a completely random design with two treatments, four replications, with measures repeated in time (July/1, Aug/1, Sept/1 and Oct/1. Buffel grass dry mater (DM availability was higher in the sheep grazed than in the goat-grazed area. Buffel grass DM supply did not change from July to September, while dicotyledons DM supply decreased. Grazing affected availability, accumulation rate and supply of the forage produced (DM basis by dicotyledonous herbs. Buffel grass forage availability was not affected during the experimental period.Objetivou-se avaliar a oferta de forragem do estrato herbáceo de uma Caatinga raleada e enriquecida com capim buffel (Cenchrus ciliaris L., submetida ao pastejo de caprinos e ovinos. O experimento foi realizado na Universidade Federal de Campina Grande, Estado da Paraíba. A área experimental foi de 2,4 ha, dividida em quatro piquetes de 0,6 ha, nos quais foram alocadas parcelas experimentais de 0,3 ha. Utilizaram-se 12 caprinos F1 (Bôer x SRD e 12 ovinos Santa Inês, que foram distribuídos em quatro grupos de seis animais. A vegetação herbácea foi separada em capim buffel (Cenchrus ciliaris L., dicotiledôneas e outras gramínea. Utilizou-se um delineamento inteiramente casualizado, com observações repetidas no tempo (1/jul., 1/ago., 1

  13. Exhaled breath analysis using electronic nose in cystic fibrosis and primary ciliary dyskinesia patients with chronic pulmonary infections

    DEFF Research Database (Denmark)

    Joensen, Odin; Paff, Tamara; Haarman, Eric G;

    2014-01-01

    of CF patients having a chronic P. aeruginosa infection differed significantly from to non-chronically infected CF patients p = 0.044. We confirmed the previously established discriminative power of exhaled breath analysis in separation between healthy subjects and patients with CF or PCD. Furthermore...... (CF) and primary ciliary dyskinesia (PCD) with or without various well characterized chronic pulmonary infections. We recruited 64 patients with CF and 21 with PCD based on known chronic infection status. 21 healthy volunteers served as controls. An electronic nose was employed to analyze exhaled...... breath samples. Principal component reduction and discriminant analysis were used to construct internally cross-validated receiver operator characteristic (ROC) curves. Breath profiles of CF and PCD patients differed significantly from healthy controls p = 0.001 and p = 0.005, respectively. Profiles...

  14. Cross-talk between ciliary epithelium and trabecular meshwork cells in-vitro: a new insight into glaucoma.

    Directory of Open Access Journals (Sweden)

    Natalie Lerner

    Full Text Available PURPOSE: It is assumed that the non-pigmented ciliary epithelium plays a role in regulating intraocular pressure via its neuroendocrine activities. To test this hypothesis, we investigated the effect on a human trabecular meshwork (TM cell line (NTM of co-culture with a human non-pigmented ciliary epithelium cell line (ODM-2. METHODS: The cellular cross-talk between ODM-2 and NTM cells was studied in a co-culture system in which the two cell types were co-cultured for 5 to 60 min or 2, 4 and 8h and then removed from the co-culture and analyzed. Analyses of the ERK and p38 mitogen-activated protein kinase (MAPK pathways and of the activity of TM phosphatases and matrix metalloproteins (MMPs were performed. Acid and alkaline phosphatase activity was determined by the DiFMUP (6, 8-difluoro-4-methylumbelliferyl phosphate assay. MMP levels were determined by gelatin zymography. RESULTS: Exposure of NTM cells to ODM-2 cells led to the activation of the MAPK signal transduction pathways in NTM cells within 5 min of co-culture. Phosphorylation of ERK1/ERK2 and p38 peaked at 10 and 15 min and then decreased over time. Interaction between ODM-2 and NTM cells promoted the expression of MMP-9 in the NTM cells after 4h of co-culture. CONCLUSIONS: Our findings provide support for the hypothesis that crosstalk does indeed take place between ODM-2 and NTM cells. Future studies should be designed to determine the relationship between the MMP system, MAPK kinases and phosphatases. Manipulation of these signaling molecules and the related NTM signal transduction pathways may provide targets for developing improved treatments for glaucoma.

  15. The ciliary inner dynein arm, I1 dynein, is assembled in the cytoplasm and transported by IFT before axonemal docking.

    Science.gov (United States)

    Viswanadha, Rasagnya; Hunter, Emily L; Yamamoto, Ryosuke; Wirschell, Maureen; Alford, Lea M; Dutcher, Susan K; Sale, Winfield S

    2014-10-01

    To determine mechanisms of assembly of ciliary dyneins, we focused on the Chlamydomonas inner dynein arm, I1 dynein, also known as dynein f. I1 dynein assembles in the cytoplasm as a 20S complex similar to the 20S I1 dynein complex isolated from the axoneme. The intermediate chain subunit, IC140 (IDA7), and heavy chains (IDA1, IDA2) are required for 20S I1 dynein preassembly in the cytoplasm. Unlike I1 dynein derived from the axoneme, the cytoplasmic 20S I1 complex will not rebind I1-deficient axonemes in vitro. To test the hypothesis that I1 dynein is transported to the distal tip of the cilia for assembly in the axoneme, we performed cytoplasmic complementation in dikaryons formed between wild-type and I1 dynein mutant cells. Rescue of I1 dynein assembly in mutant cilia occurred first at the distal tip and then proceeded toward the proximal axoneme. Notably, in contrast to other combinations, I1 dynein assembly was significantly delayed in dikaryons formed between ida7 and ida3. Furthermore, rescue of I1 dynein assembly required new protein synthesis in the ida7 × ida3 dikaryons. On the basis of the additional observations, we postulate that IDA3 is required for 20S I1 dynein transport. Cytoplasmic complementation in dikaryons using the conditional kinesin-2 mutant, fla10-1 revealed that transport of I1 dynein is dependent on kinesin-2 activity. Thus, I1 dynein complex assembly depends upon IFT for transport to the ciliary distal tip prior to docking in the axoneme.

  16. Geometric Clutch model version 3: the role of the inner and outer arm dyneins in the ciliary beat.

    Science.gov (United States)

    Lindemann, Charles B

    2002-08-01

    The Geometric Clutch model of ciliary and flagellar beating uses the transverse force (t-force) that develops between the outer doublets of the axoneme as the regulator for activating and deactivating the dynein motors and organizing the flagellar beat. The version of the model described here adds detail to the formulations used in the two previous versions as follows: (1) In place of two opposing sets of dyneins, the new model has four sets of dyneins, corresponding to two sets on each side of the axoneme acting in series. (2) The four sets of dyneins are each subdivided into two ranks representing inner and outer arm dyneins. (3) The force produced by each dynein is governed by a force-velocity relationship that is independently specified for the inner and outer arms. Consistent with the original model, the new version of the Geometric Clutch model can simulate both the effective and recovery stroke phases of the ciliary beat using a single uniform algorithm. In addition, the new version can operate with the outer arms disabled. Under this condition, the simulation exhibits a beat pattern similar to the original but the beat frequency is reduced to approximately one third. These results are contingent on using force-velocity relationships for the inner and outer arms similar to those described by Brokaw [1999: Cell Motil. Cytoskeleton 42:134-148], where the inner arms contribute most of the driving force at low shear velocities. This constitutes the first examination of the effects of the force-velocity characteristics of dynein on a cilia-like beat in a theoretical framework. PMID:12112138

  17. Inhibitory Effect of Dexamethasone on TGF-β1 Expression of Rabbit Ciliary Pigment Epithelia Cultured in Vitro

    Institute of Scientific and Technical Information of China (English)

    XIONG Xinchun; XI Zulian; MIAO Juan; LI Qinglin; ZHANG Haijiang; HU Yizhen; WEI Houren

    2006-01-01

    In order to elucidate the effect of dexamethasone on the expression of transforming growth factor-beta1 (TGF-β1) in ciliary pigment epithelial (CPE) cells cultured in vitro, rabbit CPE cells were cultured in vitro, treated with DMEM medium containing 0,1×10-8 , 5×10-8 , 10 × 10-8 and 50×10-8 mol/L dexamethasone respectively for 5 days. The TGF-β1 expression was detected by immunohistochemistry Supervision methods and analyzed semi-quantitatively by HMIAS-2000 image system. As opposed to in vivo, rabbit CPE cells expressed TGF-β1 under cultured circumstance in vitro. The gray scales of the positive yellow staining in the groups of 1×10-8, 5×10-8, 10×10-8 and 50× 10-8 mol/L dexamethasone were 136. 57±4.43, 140. 20±6.10, 142.98±2. 99, 146. 80± 1.68 and 150. 05± 1.94 respectively. When the concentrations of dexamethasone were equal to or higher than 5×10-8 mol/L and, the expression of TGF-β1 was inhibited. 10-7 mol/L dexamethasone showed a significant inhibition. It was suggested that CPE cells possess the potential ability of synthesizing and expressing TGF-β1. The inhibition of TGF-β1 expression by dexamethasone may be beneficial to the treatment of proliferative vitroretinopathy, also exert some influence on the secretion of aqueous humor and ciliary inflammation.

  18. Body Hygiene

    Science.gov (United States)

    ... Home Diaper-Changing Steps for Childcare Settings Body Hygiene Dental Hygiene Water Fluoridation Facial Cleanliness Fish Pedicures and ... spread of hygiene-related diseases . Topics for Body Hygiene Facial Cleanliness Dental Hygiene Water Fluoridation Fish Pedicures and Fish Spas ...

  19. Body Image

    Science.gov (United States)

    ... Help your child have a healthy body image Cosmetic surgery Breast surgery Botox Liposuction Varicose or spider veins Body dysmorphic disorder (BDD) Eating disorders Anorexia nervosa Binge eating ... nervosa Cosmetics and your health Depression during and after pregnancy ...

  20. Body Basics

    Science.gov (United States)

    ... about how the body works, what basic human anatomy is, and what happens when parts of the body don't function properly. Blood Bones, Muscles, and Joints Brain and Nervous System Digestive System Endocrine System Eyes Female Reproductive System ...

  1. Body embellishment

    OpenAIRE

    Zellweger, Christoph

    2015-01-01

    The exhibition Body Embellishment explores the most innovative artistic expression in the 21st-century international arenas of body extension, augmentation, and modification, focusing on jewelry, tattoos, nail arts, and fashion. The areas of focus are jewelry, tattoos, nail arts, and fashion. Avant-garde jewelry consciously engages the body by intersecting and expanding the planes of the human form. Tattoos are at once on and in the body. Nail art, from manicures to pedicures, has humble ...

  2. Body Clock

    Institute of Scientific and Technical Information of China (English)

    刘洪毓

    2000-01-01

    Body clocks” are biological methods of controling body activities.Every living thing has one. In humans, a body clock controls normal periods of sleeping and waking. It controls the time swhen you are most likely to feel pain.Eating, sleeping and exercising at about the same time each day will help keep body activities normal. But changes in your life, a new job, for example, destroy the balance and thus cause health problems.

  3. In vitro studies of antimicrobial activity of crude extracts of the Indian grasses Dhaman (Cenchrus ciliaris and Kala-Dhaman (Cenchrus setigerus

    Directory of Open Access Journals (Sweden)

    Premlata Singariya

    2012-01-01

    Full Text Available The aim of present study was to investigate the antimicrobial activity of Cenchrus ciliaris and Cenchrus setigerus extracts in order to use it as a possible source for new antimicrobial substances against important human pathogens. Crude extracts of the stem of Cenchrus ciliaris and Cenchrus setigerus were evaluated against some medically important pathogens viz. Escherichia coli, Raoultella planticola, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Enterobacter aerogenes, Candida albicans and Aspergillus flavus. The dried and powdered stems were successively extracted with hexane, toluene, isopropyl alcohol, acetone and ethanol using soxhlet assembly. The antimicrobial activity assay was done by both disc diffusion and serial dilution methods. Isopropyl alcohol extract of Cenchrus setigerus showed highest activity against Escherichia coli. The test pathogens were more sensitive to the isopropyl alcohol, acetone and ethanol extracts than to the hexane and toluene extracts except against Bacillus subtilis. Result reveals that the most bioactive compound was cycloleucolenol-9,19-cycloergost-24 (28-en-3-ol, 4, 14-dimethyl acetate in both the species of Cenchrus grass, (19.15% in isopropanol extract of Cenchrus setigerus whereas, (14.03% in acetone extract of Cenchrus ciliaris.

  4. Alternativas de manejo químico da planta daninha Digitaria ciliaris resistente aos herbicidas inibidores da ACCase na cultura de soja Chemical management alternatives of the weed Digitaria ciliaris resistant to ACCASE inhibiting herbicides in soybean crop

    Directory of Open Access Journals (Sweden)

    R.F. López-Ovejero

    2006-06-01

    Full Text Available Com o objetivo de avaliar a eficácia dos herbicidas inibidores da ACCase sobre uma população de capim-colchão (Digitaria ciliaris com histórico de falha de controle, bem como propor herbicidas alternativos a serem aplicados em condições de pré e pós-emergência na cultura de soja, foram conduzidos dois experimentos a campo no município de Palmeira-PR, durante o ano agrícola 2003/2004. No primeiro experimento, foi avaliada a eficácia dos tratamentos envolvendo herbicidas com mecanismo de ação de inibição da ACCase (g ha-1: sethoxydim (230, clethodim (108, butroxydim (75, tepraloxydim (100, fluazifop-p-butil (187,5, haloxyfop-r (60, propaquizafop (125, cyhalofop-butyl (225, fenoxaprop-p-ethyl + clethodim (50 + 50, além de testemunha sem herbicidas. No segundo experimento, os tratamentos constaram de herbicidas com mecanismos de ação alternativos, visando também o teste de eficácia de controle da planta daninha (g ha-1: trifluralina (2.700, clomazone (1.000, smetolachlor (1.920, sulfentrazone (600, trifluralina + sulfentrazone (2.100 + 400, clomazone + sulfentrazone (600 + 400, S-metolachlor + sulfentrazone (768 + 400 aplicados em condições de pré-emergência da planta daninha e da cultura e testemunha sem controle de plantas daninhas; os tratamentos apresentavam-se com ou sem complementação de controle através do herbicida imazethapyr (100, aplicado em condições de pós-emergência, com as plantas daninhas no estádio de duas a quatro folhas. Os resultados sugerem que a população estudada é resistente aos herbicidas inibidores da ACCase; os melhores resultados de eficácia de controle com os inibidores da ACCase foram obtidos com os herbicidas tepraloxydim, clethodim e butroxydim; os tratamentos com sulfentrazone isoladamente ou em mistura e os tratamentos com trifluralina, clomazone e S-metolachlor, em complementação com imazethapyr e imazethapyr isolado, foram eficazes no controle do biótipo resistente de

  5. Exhaled breath analysis using electronic nose in cystic fibrosis and primary ciliary dyskinesia patients with chronic pulmonary infections.

    Directory of Open Access Journals (Sweden)

    Odin Joensen

    Full Text Available The current diagnostic work-up and monitoring of pulmonary infections may be perceived as invasive, is time consuming and expensive. In this explorative study, we investigated whether or not a non-invasive exhaled breath analysis using an electronic nose would discriminate between cystic fibrosis (CF and primary ciliary dyskinesia (PCD with or without various well characterized chronic pulmonary infections. We recruited 64 patients with CF and 21 with PCD based on known chronic infection status. 21 healthy volunteers served as controls. An electronic nose was employed to analyze exhaled breath samples. Principal component reduction and discriminant analysis were used to construct internally cross-validated receiver operator characteristic (ROC curves. Breath profiles of CF and PCD patients differed significantly from healthy controls p = 0.001 and p = 0.005, respectively. Profiles of CF patients having a chronic P. aeruginosa infection differed significantly from to non-chronically infected CF patients p = 0.044. We confirmed the previously established discriminative power of exhaled breath analysis in separation between healthy subjects and patients with CF or PCD. Furthermore, this method significantly discriminates CF patients suffering from a chronic pulmonary P. aeruginosa (PA infection from CF patients without a chronic pulmonary infection. Further studies are needed for verification and to investigate the role of electronic nose technology in the very early diagnostic workup of pulmonary infections before the establishment of a chronic infection.

  6. The adult retinal stem cell is a rare cell in the ciliary epithelium whose progeny can differentiate into photoreceptors

    Directory of Open Access Journals (Sweden)

    Brian G. Ballios

    2012-02-01

    Self-renewing, multipotential retinal stem cells (RSCs reside in the pigmented ciliary epithelium of the peripheral retina in adult mammals. RSCs can give rise to rhodopsin positive-cells, which can integrate into early postnatal retina, and represent a potentially useful option for cellular therapy. The ability to purify a stem cell population and direct the differentiation toward a particular cell lineage is a challenge facing the application of stem cells in regenerative medicine. Here we use cell sorting to prospectively enrich mouse RSCs based on size, granularity and low expression of P-cadherin and demonstrate that only rare cells with defined properties proliferate to form colonies. We show that clonally-derived mouse and human RSC progeny are multipotent and can differentiate into mature rhodopsin-positive cells with high efficiency using combinations of exogenous culture additives known to influence neural retinal development, including taurine and retinoic acid. This directed RSC differentiation follows the temporal sequence of photoreceptor differentiation in vivo, and the cells exhibit morphology, protein and gene expression consistent with primary cultures of rods in vitro. These results demonstrate that the RSC, an adult stem cell, can be enriched and directed to produce photoreceptors as a first step toward a targeted cell replacement strategy to treat retinal degenerative disease.

  7. Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genes

    Science.gov (United States)

    Stolc, Viktor; Samanta, Manoj Pratim; Tongprasit, Waraporn; Marshall, Wallace F.

    2005-01-01

    The important role that cilia and flagella play in human disease creates an urgent need to identify genes involved in ciliary assembly and function. The strong and specific induction of flagellar-coding genes during flagellar regeneration in Chlamydomonas reinhardtii suggests that transcriptional profiling of such cells would reveal new flagella-related genes. We have conducted a genome-wide analysis of RNA transcript levels during flagellar regeneration in Chlamydomonas by using maskless photolithography method-produced DNA oligonucleotide microarrays with unique probe sequences for all exons of the 19,803 predicted genes. This analysis represents previously uncharacterized whole-genome transcriptional activity profiling study in this important model organism. Analysis of strongly induced genes reveals a large set of known flagellar components and also identifies a number of important disease-related proteins as being involved with cilia and flagella, including the zebrafish polycystic kidney genes Qilin, Reptin, and Pontin, as well as the testis-expressed tubby-like protein TULP2.

  8. Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome: The Differentiation Factors Characterization for Retinal Ganglion Cell line

    Directory of Open Access Journals (Sweden)

    Ming-Hui Yang

    2011-01-01

    Full Text Available The purpose of this paper was to characterize proteins secreted from the human nonpigmented ciliary epithelial (HNPE cells, which have differentiated a rat retinal ganglion cell line, RGC-5. Undifferentiated RGC-5 cells have been shown to express several marker proteins characteristic of retinal ganglion cells. However, RGC-5 cells do not respond to N-methyl-D aspartate (NMDA, or glutamate. HNPE cells have been shown to secrete numbers of neuropeptides or neuroproteins also found in the aqueous humor, many of which have the ability to influence the activity of neuronal cells. This paper details the profile of HNPE cell-secreted proteins by proteomic approaches. The experimental results revealed the identification of 132 unique proteins from the HNPE cell-conditioned SF-medium. The biological functions of a portion of these identified proteins are involved in cell differentiation. We hypothesized that a differentiation system of HNPE cell-conditioned SF-medium with RGC-5 cells can induce a differentiated phenotype in RGC-5 cells, with functional characteristics that more closely resemble primary cultures of rat retinal ganglion cells. These proteins may replace harsh chemicals, which are currently used to induce cell differentiation.

  9. Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom STED microscope

    Science.gov (United States)

    Meyer, Stephanie A.; Ozbay, Baris; Restrepo, Diego; Gibson, Emily A.

    2014-03-01

    We performed super-resolution imaging of isolated olfactory sensory neurons (OSNs) using a custom-built Stimulated Emission Depletion (STED) microscope. The design for the STED microscope is based on the system developed in the laboratory of Dr. Stefan Hell1. Our system is capable of imaging with sub-diffraction limited resolution simultaneously in two color channels (at Atto 590/Atto 647N wavelengths). A single, pulsed laser source (ALP; Fianium, Inc.) generates all four laser beams, two excitation and two STED. The two STED beams are coupled into one polarization maintaining (PM) fiber and the two excitation beams into another. They are then collimated and both STED beams pass through a vortex phase plate (RPC Photonics) to allow shaping into a donut at the focus of the objective lens. The beams are then combined and sent into an inverted research microscope (IX-71; Olympus Inc.) allowing widefield epifluorescence, brightfield and DIC imaging on the same field of view as STED imaging. A fast piezo stage scans the sample during STED and confocal imaging. The fluorescent signals from the two color channels are detected with two avalanche photodiodes (APD) after appropriate spectral filtering. The resolution of the system was characterized by imaging 40 nm fluorescent beads as ~60 nm (Atto 590) and ~50 nm (Atto 647N). We performed STED imaging on immunolabeled isolated OSNs tagged at the CNGA2 and ANO2 proteins. The STED microscope allows us to resolve ciliary CNGA2 microdomains of ~54 nm that were blurred in confocal.

  10. The ciliary marginal zone of the zebrafish retina: clonal and time-lapse analysis of a continuously growing tissue

    Science.gov (United States)

    Wan, Yinan; Almeida, Alexandra D.; Rulands, Steffen; Chalour, Naima; Muresan, Leila; Wu, Yunmin; Simons, Benjamin D.; He, Jie; Harris, William A.

    2016-01-01

    Clonal analysis is helping us understand the dynamics of cell replacement in homeostatic adult tissues (Simons and Clevers, 2011). Such an analysis, however, has not yet been achieved for continuously growing adult tissues, but is essential if we wish to understand the architecture of adult organs. The retinas of lower vertebrates grow throughout life from retinal stem cells (RSCs) and retinal progenitor cells (RPCs) at the rim of the retina, called the ciliary marginal zone (CMZ). Here, we show that RSCs reside in a niche at the extreme periphery of the CMZ and divide asymmetrically along a radial (peripheral to central) axis, leaving one daughter in the peripheral RSC niche and the other more central where it becomes an RPC. We also show that RPCs of the CMZ have clonal sizes and compositions that are statistically similar to progenitor cells of the embryonic retina and fit the same stochastic model of proliferation. These results link embryonic and postembryonic cell behaviour, and help to explain the constancy of tissue architecture that has been generated over a lifetime. PMID:26893352

  11. Body punk

    DEFF Research Database (Denmark)

    Mogensen, Kevin

    BODYPUNK - A Treatise on male body builders and the meaning of the body in the shadow of an Anti Doping Campaign Based on a qualitative study, the thesis investigates the visual representation of the male bodybuilder found in the national anti doping campaign: ‗ "The hunt has begun" along...... with an analysis of the embodied meaning of men‘s bodybuilding....

  12. Body punk

    DEFF Research Database (Denmark)

    Mogensen, Kevin

    BODYPUNK - A Treatise on male body builders and the meaning of the body in the shadow of an Anti Doping Campaign Based on a qualitative study, the thesis investigates the visual representation of the male bodybuilder found in the national anti doping campaign: ‗ "The hunt has begun" along with an...... analysis of the embodied meaning of men‘s bodybuilding....

  13. Body Language

    Institute of Scientific and Technical Information of China (English)

    JosephDeVeto

    2004-01-01

    When we speak,we use much more than just words. We also communicate with our face. our hands,and even our own body. This Kind of communication ean be called “body language” or “non-verbal eommunieation”. Non-verbal

  14. Body Weight and Body Image

    OpenAIRE

    McFarlane Traci; Olmsted Marion P

    2004-01-01

    Abstract Health Issue Body weight is of physical and psychological importance to Canadian women; it is associated with health status, physical activity, body image, and self-esteem. Although the problems associated with overweight and obesity are indeed serious, there are also problems connected to being underweight. Weight prejudice and the dieting industry intensify body image concerns for Canadian women and can have a major negative impact on self-esteem. Key Findings Women have lower BMIs...

  15. Bog bodies

    DEFF Research Database (Denmark)

    Lynnerup, Niels

    2015-01-01

    the bog bodies have been studied using medical and natural scientific methods, and recently many bog bodies have been re-examined using especially modern, medical imaging techniques. Because of the preservation of soft tissue, especially the skin, it has been possible to determine lesions and trauma...... and shrink, leading to potential pitfalls in paleopathological diagnostics. Bog bodies have in several instances been crucial in determining the last meal, as gut contents may be preserved, and thus augment our knowledge on pre-historic diet by adding to, for example, stable isotope analyses. This article...

  16. Response of nitrogen fixation in relation to nodule carbohydrate metabolism in Medicago ciliaris lines subjected to salt stress.

    Science.gov (United States)

    Ben Salah, Imène; Albacete, Alfonso; Martínez Andújar, Cristina; Haouala, Rabiaa; Labidi, Nehla; Zribi, Fethia; Martinez, Vicente; Pérez-Alfocea, Francisco; Abdelly, Chedly

    2009-03-15

    The effect of salt stress on nitrogen fixation, in relation to sucrose transport towards nodules and other sink organs and the potential of sucrose breakdown by nodules, was investigated in two lines of Medicago ciliaris. Under salt stress conditions, the two lines showed a decrease of total biomass production, but TNC 1.8 was less affected by salt than TNC 11.9. The chlorophyll content was not changed in TNC 1.8, in contrast to TNC 11.9. Shoot, root, and nodule biomass were also affected in the two lines, but TNC 1.8 exhibited the higher potentialities of biomass production of these organs. Nitrogen fixation also decreased in the two lines, and was more sensitive to salt than growth parameters. TNC 1.8 consistently exhibited the higher values of nitrogen fixation. Unlike nodules, leaves of both lines were well supplied in nutrients with some exceptions. Specifically, the calcium content decreased in the sensitive line leaves, and the nodule magnesium content was not changed in either line. The tolerant line accumulated more sodium in its leaves. The two lines did not show any differences in the nodule sodium content. Sucrose allocation towards nodules was affected by salt in the two lines, but this constraint did not seem to affect the repartition of sucrose between sink organs. Salt stress induced perturbations in nodule sucrolytic activities in the two lines. It inhibited sucrose synthase, but the inhibition was more marked in TNC 11.9; alkaline/neutral activity was not altered in TNC 1.8, whereas it decreased more than half in TNC 11.9. Thus, the relative tolerance of TNC 1.8 to salt stress could be attributed to a better use of these photoassimilates by nodules and a better supply of bacteroids in malate. The hypothesis of a competition for sucrose between nodules and other sink organs under salt stress could not be verified. PMID:18804311

  17. Body Language

    Institute of Scientific and Technical Information of China (English)

    王芳

    2008-01-01

    @@ For Teachers: The Wordless Language Spoken by Everyone by Pamela Osment An old saying goes:"Actions speak louder than words."That's true according to communication experts.Some studies show that up to 90 percent of communication is nonverbal.Though you might say one thing,your body movements may indicate something entirely different.This nonverbal way of communicating is called body language.The Universal(通用的)Language

  18. 人工晶体睫状沟缝合固定术临床观察%Clinical observation of intraocular lens ciliary sulcus suture fixation

    Institute of Scientific and Technical Information of China (English)

    沙英虹; 史要武

    2015-01-01

    Objective To investigate clinical application of intraocular lens ciliary sulcus suture fixation.Methods Posterior chamber intraocular lens ciliary sulcus suture fixation was applied for 15 patients with traumatic lens subluxation, posterior capsule injury, posterior capsule injury after senile cataract ultrasonic emulsification, and non-lens after vitrectomy. Their clinical effects were observed.Results Follow-up for 6 months showed postoperative visual acuity in 15 cases were all better than best corrected visual acuity before operation.Conclusion Implement of intraocular lens ciliary sulcus suture fixation is a safe and effective method for non-lens capsule, lens posterior capsular rupture, or wide acantholysis of lens suspensory ligament.%目的 探讨人工晶状体睫状沟缝合固定术的临床应用.方法 对15例外伤性晶体半脱位,后囊破损,老年性白内障超声乳化后囊破损,玻璃体切割术后无晶体眼患者施行后房型人工晶体(IOL)缝线固定术,观察临床效果.结果 随诊6个月,15例患者术后视力均高于术前最佳矫正视力.结论 对无晶体囊膜及晶体后囊膜破裂或晶体悬韧带大范围松解施行人工晶状体睫状沟固定术是一种安全有效的方法.

  19. Ciliary dyslexia candidate genes DYX1C1 and DCDC2 are regulated by Regulatory Factor X (RFX) transcription factors through X-box promoter motifs

    Science.gov (United States)

    Tammimies, Kristiina; Bieder, Andrea; Lauter, Gilbert; Sugiaman-Trapman, Debora; Torchet, Rachel; Hokkanen, Marie-Estelle; Burghoorn, Jan; Castrén, Eero; Kere, Juha; Tapia-Páez, Isabel; Swoboda, Peter

    2016-01-01

    DYX1C1, DCDC2, and KIAA0319 are three of the most replicated dyslexia candidate genes (DCGs). Recently, these DCGs were implicated in functions at the cilium. Here, we investigate the regulation of these DCGs by Regulatory Factor X transcription factors (RFX TFs), a gene family known for transcriptionally regulating ciliary genes. We identify conserved X-box motifs in the promoter regions of DYX1C1, DCDC2, and KIAA0319 and demonstrate their functionality, as well as the ability to recruit RFX TFs using reporter gene and electrophoretic mobility shift assays. Furthermore, we uncover a complex regulation pattern between RFX1, RFX2, and RFX3 and their significant effect on modifying the endogenous expression of DYX1C1 and DCDC2 in a human retinal pigmented epithelial cell line immortalized with hTERT (hTERT-RPE1). In addition, induction of ciliogenesis increases the expression of RFX TFs and DCGs. At the protein level, we show that endogenous DYX1C1 localizes to the base of the cilium, whereas DCDC2 localizes along the entire axoneme of the cilium, thereby validating earlier localization studies using overexpression models. Our results corroborate the emerging role of DCGs in ciliary function and characterize functional noncoding elements, X-box promoter motifs, in DCG promoter regions, which thus can be targeted for mutation screening in dyslexia and ciliopathies associated with these genes.—Tammimies, K., Bieder, A., Lauter, G., Sugiaman-Trapman, D., Torchet, R., Hokkanen, M.-E., Burghoorn, J., Castrén, E., Kere, J., Tapia-Páez, I., Swoboda, P. Ciliary dyslexia candidate genes DYX1C1 and DCDC2 are regulated by Regulatory Factor (RF) X transcription factors through X-box promoter motifs. PMID:27451412

  20. Impact of Combination Use of 0.004%Travoprost and 2%Pilocarpine on Matrix Metalloproteinases Synthesized by Rabbit Ciliary Muscle:a Pilot Study

    Institute of Scientific and Technical Information of China (English)

    Shun-hua Zhang; Jia-liang Zhao

    2013-01-01

    Objective To explore the impact of combination use of prostaglandin analogue and cholinergic agonists on main matrix metalloproteinases (MMPs) synthesized by albino rabbit ciliary muscle. Methods Normal adult albino rabbits were divided into the control group, 2%pilocarpine group, 0.004% travoprost group and travoprost plus pilocarpine group. Two rabbits in the control group were executed after treated with normal saline for one day. Two rabbits were separately executed on the 7th, 14th and 24th day of the treatment in each drug treated group. In each subgroup ciliary muscle band of 4 eyes was taken and made into homogenate. The MMPs activities of 10 subgroups were assayed by zymography. Bands’ intensity which represents the activity of MMPs was measured by the UltraViolet Illumination system. Results A bright band of MMP-1/2 was showed on each lane at the position corresponding to the molecular weight of 62 kD in the ciliary smooth muscles electrophoresis. When ion Zn and Ca was displaced by MMPs inhibitor EDTA, this bright band disappeared. Compared with the control group, MMP1/2 activity increased by 4.0%, 4.1%and 14.0%after 7, 14 and 24 days of pilocarpine treatment. Corresponding data was 23.2%, 61.7% and 111.5% in the travoprost group and 49.3%, 68.0% and 88.4% in the travoprost plus pilocarpine group. Conclusions Pilocarpine has little effect on activity of MMP1/2. Travoprost can increase activity of MMP1/2 gradually. Activity of MMP1/2 is rapidly increased by pilocarpine combined with travoprost, but shows small change with the prolonged treatment.

  1. Adenylate Kinase and AMP Signaling Networks: Metabolic Monitoring, Signal Communication and Body Energy Sensing

    Directory of Open Access Journals (Sweden)

    Andre Terzic

    2009-04-01

    Full Text Available Adenylate kinase and downstream AMP signaling is an integrated metabolic monitoring system which reads the cellular energy state in order to tune and report signals to metabolic sensors. A network of adenylate kinase isoforms (AK1-AK7 are distributed throughout intracellular compartments, interstitial space and body fluids to regulate energetic and metabolic signaling circuits, securing efficient cell energy economy, signal communication and stress response. The dynamics of adenylate kinase-catalyzed phosphotransfer regulates multiple intracellular and extracellular energy-dependent and nucleotide signaling processes, including excitation-contraction coupling, hormone secretion, cell and ciliary motility, nuclear transport, energetics of cell cycle, DNA synthesis and repair, and developmental programming. Metabolomic analyses indicate that cellular, interstitial and blood AMP levels are potential metabolic signals associated with vital functions including body energy sensing, sleep, hibernation and food intake. Either low or excess AMP signaling has been linked to human disease such as diabetes, obesity and hypertrophic cardiomyopathy. Recent studies indicate that derangements in adenylate kinase-mediated energetic signaling due to mutations in AK1, AK2 or AK7 isoforms are associated with hemolytic anemia, reticular dysgenesis and ciliary dyskinesia. Moreover, hormonal, food and antidiabetic drug actions are frequently coupled to alterations of cellular AMP levels and associated signaling. Thus, by monitoring energy state and generating and distributing AMP metabolic signals adenylate kinase represents a unique hub within the cellular homeostatic network.

  2. Zebrafish Ciliopathy Screen Plus Human Mutational Analysis Identifies C21orf59 and CCDC65 Defects as Causing Primary Ciliary Dyskinesia

    OpenAIRE

    Austin-Tse, Christina; Halbritter, Jan; Zariwala, Maimoona A.; Gilberti, Renée M.; Gee, Heon Yung; Hellman, Nathan; Pathak, Narendra; Liu, Yan; Panizzi, Jennifer R.; Patel-King, Ramila S.; Tritschler, Douglas; Bower, Raqual; O’Toole, Eileen; Porath, Jonathan D.; Hurd, Toby W.

    2013-01-01

    Primary ciliary dyskinesia (PCD) is caused when defects of motile cilia lead to chronic airway infections, male infertility, and situs abnormalities. Multiple causative PCD mutations account for only 65% of cases, suggesting that many genes essential for cilia function remain to be discovered. By using zebrafish morpholino knockdown of PCD candidate genes as an in vivo screening platform, we identified c21orf59, ccdc65, and c15orf26 as critical for cilia motility. c21orf59 and c15orf26 knockd...

  3. Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-α/β1-Independent

    Science.gov (United States)

    Torrado, Belén; Graña, Martín; Badano, José L.; Irigoín, Florencia

    2016-01-01

    Gli2 is the primary transcriptional activator of Hedgehog signalling in mammals. Upon stimulation of the pathway, Gli2 moves into the cilium before reaching the nucleus. However, the mechanisms underlying its entry into the cilium are not completely understood. Since several similarities have been reported between nuclear and ciliary import, we investigated if the nuclear import machinery participates in Gli2 ciliary entry. Here we show that while two conserved classical nuclear localization signals mediate Gli2 nuclear localization via importin (Imp)-α/β1, these sequences are not required for Gli2 ciliary import. However, blocking Imp-mediated transport through overexpression of GTP-locked Ran reduced the percentage of Gli2 positive cilia, an effect that was not explained by increased CRM1-dependent export of Gli2 from the cilium. We explored the participation of Imp-β2 in Gli2 ciliary traffic and observed that this transporter is involved in moving Gli2 into the cilium, as has been described for other ciliary proteins. In addition, our data indicate that Imp-β2 might also collaborate in Gli2 nuclear entry. How does Imp-β2 determine the final destination of a protein that can localize to two distinct subcellular compartments remains an open question. Therefore, our data shows that the nuclear-cytoplasmic shuttling machinery plays a critical role mediating the subcellular distribution of Gli2 and the activation of the pathway, but distinct importins likely play a differential role mediating its ciliary and nuclear translocation. PMID:27579771

  4. Body contact and body language

    DEFF Research Database (Denmark)

    Winther, Helle Dagmar

    2008-01-01

    ­ment psychology and education provide potential for intense personal develop­ment as well as for social and cultural learning processes. This performative research project originates from the research project entitled, Movement Psy­chol­ogy: The Language of the Body and the Psy­chol­ogy of Movement based...... on the Dance Therapy Form Dansergia. The author, who is a practi­tioner-researcher, is methodologically inspir­ed by phenomenology, performative methods and a narrative and auto-ethnographic approach. The project will be presented in an organic, cre­at­ive and performative way. Through a moving dia......Body contact and body language are unique and existential and, although culturally dependent and socially embodied, they are also universal communication forms. For small children all over the world, warm, close and nourishing body contact is fundamental to their embodied experi­ence of themselves...

  5. Body Imaging

    Science.gov (United States)

    2001-01-01

    The high-tech art of digital signal processing (DSP) was pioneered at NASA's Jet Propulsion Laboratory (JPL) in the mid-1960s for use in the Apollo Lunar Landing Program. Designed to computer enhance pictures of the Moon, this technology became the basis for the Landsat Earth resources satellites and subsequently has been incorporated into a broad range of Earthbound medical and diagnostic tools. DSP is employed in advanced body imaging techniques including Computer-Aided Tomography, also known as CT and CATScan, and Magnetic Resonance Imaging (MRI). CT images are collected by irradiating a thin slice of the body with a fan-shaped x-ray beam from a number of directions around the body's perimeter. A tomographic (slice-like) picture is reconstructed from these multiple views by a computer. MRI employs a magnetic field and radio waves, rather than x-rays, to create images.

  6. Body Rainbow

    Institute of Scientific and Technical Information of China (English)

    2014-01-01

    Phubu did not know how long hehad walked after leaving Baxoi, buthe did know that he was halfwaybetween home and Lhasa. Feelingthe weight of the sack containingPhumo's body on his back, Fhubuhad calmed down from the grief anddesperation. He had just one wish:to carry Phumo to Lhasa. He knewthat Phumo had gone, and her soulwas no longer in this body. But hewas determined to finish the trip, notonly because he had promised so, butalso that he believed that it would beredemption for him.

  7. Susceptible Bodies

    DEFF Research Database (Denmark)

    Grünenberg, Kristina

    2014-01-01

    is understood as ‘susceptible bodies’ by acupuncturists and reflexologists, are brought into being through various bodily and narrative practices. From a practitioners’ perspective, these practices potentially influence treatment outcomes and enables the emergence of not just new bodies, but also potentially...

  8. Cell context-specific expression of primary cilia in the human testis and ciliary coordination of Hedgehog signalling in mouse Leydig cells

    DEFF Research Database (Denmark)

    Berg Nygaard, Marie; Almstrup, Kristian; Lindbæk, Louise;

    2015-01-01

    Primary cilia are sensory organelles that coordinate numerous cellular signalling pathways during development and adulthood. Defects in ciliary assembly or function lead to a series of developmental disorders and diseases commonly referred to as ciliopathies. Still, little is known about the form......Primary cilia are sensory organelles that coordinate numerous cellular signalling pathways during development and adulthood. Defects in ciliary assembly or function lead to a series of developmental disorders and diseases commonly referred to as ciliopathies. Still, little is known about...... the formation and function of primary cilia in the mammalian testis. Here, we characterized primary cilia in adult human testis and report a constitutive expression of cilia in peritubular myoid cells and a dynamic expression of cilia in differentiating Leydig cells. Primary cilia are generally absent from...... cells of mature seminiferous epithelium, but present in Sertoli cell-only tubules in Klinefelter syndrome testis. Peritubular cells in atrophic testis produce overly long cilia. Furthermore cultures of growth-arrested immature mouse Leydig cells express primary cilia that are enriched in components...

  9. Mutations in GAS8, a Gene Encoding a Nexin-Dynein Regulatory Complex Subunit, Cause Primary Ciliary Dyskinesia with Axonemal Disorganization.

    Science.gov (United States)

    Jeanson, Ludovic; Thomas, Lucie; Copin, Bruno; Coste, André; Sermet-Gaudelus, Isabelle; Dastot-Le Moal, Florence; Duquesnoy, Philippe; Montantin, Guy; Collot, Nathalie; Tissier, Sylvie; Papon, Jean-François; Clement, Annick; Louis, Bruno; Escudier, Estelle; Amselem, Serge; Legendre, Marie

    2016-08-01

    Primary ciliary dyskinesia (PCD) is an autosomal recessive disease characterized by chronic respiratory infections of the upper and lower airways, hypofertility, and, in approximately half of the cases, situs inversus. This complex phenotype results from defects in motile cilia and sperm flagella. Among the numerous genes involved in PCD, very few-including CCDC39 and CCDC40-carry mutations that lead to a disorganization of ciliary axonemes with microtubule misalignment. Focusing on this particular phenotype, we identified bi-allelic loss-of-function mutations in GAS8, a gene that encodes a subunit of the nexin-dynein regulatory complex (N-DRC) orthologous to DRC4 of the flagellated alga Chlamydomonas reinhardtii. Unlike the majority of PCD patients, individuals with GAS8 mutations have motile cilia, which, as documented by high-speed videomicroscopy, display a subtle beating pattern defect characterized by slightly reduced bending amplitude. Immunofluorescence studies performed on patients' respiratory cilia revealed that GAS8 is not required for the proper expression of CCDC39 and CCDC40. Rather, mutations in GAS8 affect the subcellular localization of another N-DRC subunit called DRC3. Overall, this study, which identifies GAS8 as a PCD gene, unveils the key importance of the corresponding protein in N-DRC integrity and in the proper alignment of axonemal microtubules in humans. PMID:27120127

  10. The Oligomeric Outer Dynein Arm Assembly Factor CCDC103 Is Tightly Integrated within the Ciliary Axoneme and Exhibits Periodic Binding to Microtubules*

    Science.gov (United States)

    King, Stephen M.; Patel-King, Ramila S.

    2015-01-01

    CCDC103 is an ∼29-kDa protein consisting of a central RPAP3_C domain flanked by N- and C-terminal coiled coils. Defects in CCDC103 lead to primary ciliary dyskinesia caused by the loss of outer dynein arms. This protein is present along the entire length of the ciliary axoneme and does not require other dynein or docking complex components for its integration. Unlike other known dynein assembly factors within the axoneme, CCDC103 is not solubilized by 0.6 m NaCl and requires more chaotropic conditions, such as 0.5 m KI. Alternatively, it can be extracted using 0.3% sarkosyl. CCDC103 forms stable dimers and other oligomers in solution through interactions involving the central domain. The smallest particle observed by dynamic light scattering has a hydrodynamic diameter of ∼25 nm. Furthermore, CCDC103 binds microtubules directly, forming ∼9-nm diameter particles that exhibit a 12-nm spacing on the microtubule lattice, suggesting that there may be two CCDC103 units per outer arm dynein repeat. Although the outer dynein arm docking complex is necessary to form arrays of dyneins along microtubules, it is not sufficient to set up a single array in a precise location on each axonemal doublet. We propose that CCDC103 helps generate a high-affinity site on the doublets for outer arm assembly, either through direct interactions or indirectly, perhaps by modifying the underlying microtubule lattice. PMID:25572396

  11. Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy

    Directory of Open Access Journals (Sweden)

    Čelakovský Pavel

    2011-06-01

    Full Text Available Abstract Background It is difficult to repair nerve if proximal stump is unavailable or autogenous nerve grafts are insufficient for reconstructing extensive nerve damage. Therefore, alternative methods have been developed, including lateral anastomosis based on axons' ability to send out collateral sprouts into denervated nerve. The different capacity of a sensory or motor axon to send a sprout is controversial and may be controlled by cytokines and/or neurotrophic factors like ciliary neurotrophic factor (CNTF. The aim of the present study was to quantitatively assess collateral sprouts sent out by intact motor and sensory axons in the end-to-side neurorrhaphy model following intrathecal administration of CNTF in comparison with phosphate buffered saline (vehiculum and Cerebrolysin. The distal stump of rat transected musculocutaneous nerve (MCN was attached in an end-to-side fashion with ulnar nerve. CNTF, Cerebrolysin and vehiculum were administered intrathecally for 2 weeks, and all animals were allowed to survive for 2 months from operation. Numbers of spinal motor and dorsal root ganglia neurons were estimated following their retrograde labeling by Fluoro-Ruby and Fluoro-Emerald applied to ulnar and musculocutaneous nerve, respectively. Reinnervation of biceps brachii muscles was assessed by electromyography, behavioral test, and diameter and myelin sheath thickness of regenerated axons. Results Vehiculum or Cerebrolysin administration resulted in significantly higher numbers of myelinated axons regenerated into the MCN stumps compared with CNTF treatment. By contrast, the mean diameter of the myelinated axons and their myelin sheath thickness in the cases of Cerebrolysin- or CNTF-treated animals were larger than were those for rats treated with vehiculum. CNTF treatment significantly increased the percentage of motoneurons contributing to reinnervation of the MCN stumps (to 17.1% when compared with vehiculum or Cerebrolysin treatments (at

  12. Sacralising Bodies

    DEFF Research Database (Denmark)

    Kaur, Ravinder

    2010-01-01

    In post-revolution Iran, the sacred notion of martyrdom has been transformed into a routine act of government – a moral sign of order and state sovereignty. Moving beyond the debates of the secularisation of the sacred and the making sacred of the secular, this article argues that the moment...... of sacralisation is realised through co-production within a social setting when the object of sacralisation is recognised as such by others. In contemporary Iran, however, the moment of sacralising bodies by the state is also the moment of its own subversion as the political-theological field of martyrdom......-sacrifice became central to the mass mobilisation against the monarchy. Once the revolutionary government came into existence, this sacred tradition was regulated to create ‘martyrs’ as a fixed category, in order to consolidate the legacy of the revolution. In this political theatre, the dead body is a site...

  13. A peptide derived from the CD loop-D helix region of ciliary neurotrophic factor (CNTF) induces neuronal differentiation and survival by binding to the leukemia inhibitory factor (LIF) receptor and common cytokine receptor chain gp130

    DEFF Research Database (Denmark)

    Rathje, Mette; Pankratova, Stanislava; Nielsen, Janne;

    2011-01-01

    Ciliary neurotrophic factor (CNTF) induces neuronal differentiation and promotes the survival of various neuronal cell types by binding to a receptor complex formed by CNTF receptor a (CNTFRa), gp130, and the leukemia inhibitory factor (LIF) receptor (LIFR). The CD loop-D helix region of CNTF has...

  14. A conserved role for Notch signaling in priming the cellular response to Shh through ciliary localisation of the key Shh transducer Smo

    Science.gov (United States)

    Stasiulewicz, Magdalena; Gray, Shona D.; Mastromina, Ioanna; Silva, Joana C.; Björklund, Mia; Seymour, Philip A.; Booth, David; Thompson, Calum; Green, Richard J.; Hall, Emma A.; Serup, Palle; Dale, J. Kim

    2015-01-01

    Notochord-derived Sonic Hedgehog (Shh) is essential for dorsoventral patterning of the overlying neural tube. Increasing concentration and duration of Shh signal induces progenitors to acquire progressively more ventral fates. We show that Notch signalling augments the response of neuroepithelial cells to Shh, leading to the induction of higher expression levels of the Shh target gene Ptch1 and subsequently induction of more ventral cell fates. Furthermore, we demonstrate that activated Notch1 leads to pronounced accumulation of Smoothened (Smo) within primary cilia and elevated levels of full-length Gli3. Finally, we show that Notch activity promotes longer primary cilia both in vitro and in vivo. Strikingly, these Notch-regulated effects are Shh independent. These data identify Notch signalling as a novel modulator of Shh signalling that acts mechanistically via regulation of ciliary localisation of key components of its transduction machinery. PMID:25995356

  15. [Multifaceted body. I. The bodies of medicine].

    Science.gov (United States)

    Saraga, M; Bourquin, C; Wykretowicz, H; Stiefel, F

    2015-02-11

    The human body is the object upon which medicine is acting, but also lived reality, image, symbol, representation and the object of elaboration and theory. All these elements which constitute the body influence the way medicine is treating it. In this series of three articles, we address the human body from various perspectives: medical (1), phenomenological (2), psychosomatic and socio-anthropological (3). This first article discusses four distinct types of representation of the body within medicine, each related to a specific epistemology and shaping a distinct kind of clinical legitimacy: the body-object of anatomy, the body-machine of physiology, the cybernetic body of biology, the statistical body of epidemiology.

  16. Body Image and Body Contouring Procedures.

    Science.gov (United States)

    Sarwer, David B; Polonsky, Heather M

    2016-10-01

    Dissatisfaction with physical appearance and body image is a common psychological phenomena in Western society. Body image dissatisfaction is frequently reported by those who have excess body weight, but also is seen in those of normal body weight. For both groups of individuals, this dissatisfaction impacts self-esteem and quality of life. Furthermore, it is believed to be the motivational catalyst to a range of appearance-enhancing behaviors, including weight loss efforts and physical activity. Body image dissatisfaction is also believed to play a role in the decision to seek the wide range of body contouring procedures offered by aesthetic physicians. Individuals who seek these procedures typically report increased body image dissatisfaction, focus on the feature they wish to alter with treatment, and often experience improvement in body image following treatment. At the same time, extreme body image dissatisfaction is a symptom of a number of recognized psychiatric disorders. These include anorexia nervosa, bulimia nervosa, and body dysmorphic disorder (BDD), all of which can contraindicate aesthetic treatment. This special topic review paper provides an overview of the relationship between body image dissatisfaction and aesthetic procedures designed to improve body contouring. The review specifically focuses on the relationship of body image and body weight, as well as the presentation of body image psychopathology that would contraindicate aesthetic surgery. The overall goal of the paper is to highlight the clinical implications of the existing research and provide suggestions for future research on the psychological aspects of body contouring procedures. PMID:27634782

  17. Body Image and Body Contouring Procedures.

    Science.gov (United States)

    Sarwer, David B; Polonsky, Heather M

    2016-10-01

    Dissatisfaction with physical appearance and body image is a common psychological phenomena in Western society. Body image dissatisfaction is frequently reported by those who have excess body weight, but also is seen in those of normal body weight. For both groups of individuals, this dissatisfaction impacts self-esteem and quality of life. Furthermore, it is believed to be the motivational catalyst to a range of appearance-enhancing behaviors, including weight loss efforts and physical activity. Body image dissatisfaction is also believed to play a role in the decision to seek the wide range of body contouring procedures offered by aesthetic physicians. Individuals who seek these procedures typically report increased body image dissatisfaction, focus on the feature they wish to alter with treatment, and often experience improvement in body image following treatment. At the same time, extreme body image dissatisfaction is a symptom of a number of recognized psychiatric disorders. These include anorexia nervosa, bulimia nervosa, and body dysmorphic disorder (BDD), all of which can contraindicate aesthetic treatment. This special topic review paper provides an overview of the relationship between body image dissatisfaction and aesthetic procedures designed to improve body contouring. The review specifically focuses on the relationship of body image and body weight, as well as the presentation of body image psychopathology that would contraindicate aesthetic surgery. The overall goal of the paper is to highlight the clinical implications of the existing research and provide suggestions for future research on the psychological aspects of body contouring procedures.

  18. Dementia with Lewy Bodies

    Science.gov (United States)

    ... Awards Enhancing Diversity Find People About NINDS NINDS Dementia With Lewy Bodies Information Page Synonym(s): Lewy Body ... and Information Additional resources from MedlinePlus What is Dementia With Lewy Bodies? Dementia with Lewy bodies (DLB) ...

  19. A Conserved Role for Girdin in Basal Body Positioning and Ciliogenesis.

    Science.gov (United States)

    Nechipurenko, Inna V; Olivier-Mason, Anique; Kazatskaya, Anna; Kennedy, Julie; McLachlan, Ian G; Heiman, Maxwell G; Blacque, Oliver E; Sengupta, Piali

    2016-09-12

    Primary cilia are ubiquitous sensory organelles that mediate diverse signaling pathways. Cilia position on the cell surface is determined by the location of the basal body (BB) that templates the cilium. The mechanisms that regulate BB positioning in the context of ciliogenesis are largely unknown. Here we show that the conserved signaling and scaffolding protein Girdin localizes to the proximal regions of centrioles and regulates BB positioning and ciliogenesis in Caenorhabditis elegans sensory neurons and human RPE-1 cells. Girdin depletion alters localization of the intercentriolar linker and ciliary rootlet component rootletin, and rootletin knockdown in RPE-1 cells mimics Girdin-dependent phenotypes. C. elegans Girdin also regulates localization of the apical junction component AJM-1, suggesting that in nematodes Girdin may position BBs via rootletin- and AJM-1-dependent anchoring to the cytoskeleton and plasma membrane, respectively. Together, our results describe a conserved role for Girdin in BB positioning and ciliogenesis. PMID:27623382

  20. [Multifaceted body. 2. The lived body].

    Science.gov (United States)

    Wykretowicz, H; Saraga, M; Bourquin, C; Stiefel, F

    2015-02-11

    The human body is the object upon which medicine is acting, but also lived reality, image, symbol, representation and the object of elaboration and theory. All these elements which constitute the body influence the way medicine is treating it. In this series of three articles, we address the human body from various perspectives: medical (1), phenomenological (2), psychosomatic and socio-anthropological (3). This second article distinguishes between the body as an object of knowledge or representation and the way the body is lived. This distinction which originates in phenomenological psychiatry aims to understand how the patient experiences his body and to surpass the classical somatic and psychiatric classifications. PMID:25895215

  1. [Multifaceted body. 2. The lived body].

    Science.gov (United States)

    Wykretowicz, H; Saraga, M; Bourquin, C; Stiefel, F

    2015-02-11

    The human body is the object upon which medicine is acting, but also lived reality, image, symbol, representation and the object of elaboration and theory. All these elements which constitute the body influence the way medicine is treating it. In this series of three articles, we address the human body from various perspectives: medical (1), phenomenological (2), psychosomatic and socio-anthropological (3). This second article distinguishes between the body as an object of knowledge or representation and the way the body is lived. This distinction which originates in phenomenological psychiatry aims to understand how the patient experiences his body and to surpass the classical somatic and psychiatric classifications.

  2. [Multifaceted body. I. The bodies of medicine].

    Science.gov (United States)

    Saraga, M; Bourquin, C; Wykretowicz, H; Stiefel, F

    2015-02-11

    The human body is the object upon which medicine is acting, but also lived reality, image, symbol, representation and the object of elaboration and theory. All these elements which constitute the body influence the way medicine is treating it. In this series of three articles, we address the human body from various perspectives: medical (1), phenomenological (2), psychosomatic and socio-anthropological (3). This first article discusses four distinct types of representation of the body within medicine, each related to a specific epistemology and shaping a distinct kind of clinical legitimacy: the body-object of anatomy, the body-machine of physiology, the cybernetic body of biology, the statistical body of epidemiology. PMID:25895214

  3. 2-BODY AND 3-BODY PARQUET THEORY

    NARCIS (Netherlands)

    LANDE, A; SMITH, RA

    1992-01-01

    One of the fundamental approaches to microscopic many-body theory is through the use of perturbation theory. This paper presents a clear derivation of the equations that sum the two-body and three body reducible diagrams that are generated from some input set of irreducible diagrams (typically the b

  4. 人工晶状体睫状沟缝线固定术的临床观察%Clinical observation of ciliary sulcus fixation of intraocular lens

    Institute of Scientific and Technical Information of China (English)

    郑丁瑞

    2012-01-01

    Objective To explore the clinical efficacy of ciliary sulcus fixation of intraocular lens (IOL).Methods 15 cases ( 15 eyes) underwent sulcus fixation of posterior chamber IOL.The visual acuities,intraoperative and postoperative complications were observed.Results Postoperative corrected visual acuities:6 eyesat ≥0.5,8 eyesat0.2 ~0.4,1 eye at0.1.Conclusion Sulcus fixation of IOLis a reliable technique to treat complicated surgeries.%目的 探讨人工晶状体睫状沟缝线固定术的临床效果.方法 后房型人工晶状体(IOL)睫状沟缝线固定术15例(15眼),观察临床效果及术中、术后,近期、远期并发症.结果 术后矫正视力≥0.5者6眼,0.2 ~0.4者8眼,0.1者1眼.结论 人工晶状体睫状沟缝线固定术为特殊情况下的人工晶状体植入提供了一个可靠的固定方法.

  5. Cytokine-Like Factor 1, an Essential Facilitator of Cardiotrophin-Like Cytokine:Ciliary Neurotrophic Factor Receptor α Signaling and sorLA-Mediated Turnover

    Science.gov (United States)

    Kristensen, Anders Mejer; Pallesen, Lone Tjener; Bauer, Johannes; Vægter, Christian Bjerggaard; Nielsen, Morten Schallburg; Madsen, Peder

    2016-01-01

    Cardiotrophin-like cytokine:cytokine-like factor-1 (CLC:CLF-1) is a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development. Mice lacking CLC:CLF-1 die soon after birth due to a suckling defect and show reduced numbers of motor neurons. Humans carrying mutations in CLC:CLF-1 develop similar disorders, known as Sohar-Crisponi or cold-induced sweating syndrome, and have a high risk of early death. It is well known that CLC binds the ciliary neurotrophic factor receptor α (CNTFRα) and is a prerequisite for signaling through the gp130/leukemia inhibitory factor receptor β (LIFRβ) heterodimer, whereas CLF-1 serves to promote the cellular release of CLC. However, the precise role of CLF-1 is unclear. Here, we report that CLF-1, based on its binding site for CLC and on two additional and independent sites for CNTFRα and sorLA, is a key player in CLC and CNTFRα signaling and turnover. The site for CNTFRα enables CLF-1 to promote CLC:CNTFRα complex formation and signaling. The second site establishes a link between the endocytic receptor sorLA and the tripartite CLC:CLF-1:CNTFRα complex and allows sorLA to downregulate the CNTFRα pool in stimulated cells. Finally, sorLA may bind and concentrate the tripartite soluble CLC:CLF-1:CNTFRα complex on cell membranes and thus facilitate its signaling through gp130/LIFRβ. PMID:26858303

  6. Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom two-color stimulated emission depletion microscope

    Science.gov (United States)

    Meyer, Stephanie A.; Ozbay, Baris N.; Potcoava, Mariana; Salcedo, Ernesto; Restrepo, Diego; Gibson, Emily A.

    2016-06-01

    We performed stimulated emission depletion (STED) imaging of isolated olfactory sensory neurons (OSNs) using a custom-built microscope. The STED microscope uses a single pulsed laser to excite two separate fluorophores, Atto 590 and Atto 647N. A gated timing circuit combined with temporal interleaving of the different color excitation/STED laser pulses filters the two channel detection and greatly minimizes crosstalk. We quantified the instrument resolution to be ˜81 and ˜44 nm, for the Atto 590 and Atto 647N channels. The spatial separation between the two channels was measured to be under 10 nm, well below the resolution limit. The custom-STED microscope is incorporated onto a commercial research microscope allowing brightfield, differential interference contrast, and epifluorescence imaging on the same field of view. We performed immunolabeling of OSNs in mice to image localization of ciliary membrane proteins involved in olfactory transduction. We imaged Ca2+-permeable cyclic nucleotide gated (CNG) channel (Atto 594) and adenylyl cyclase type III (ACIII) (Atto 647N) in distinct cilia. STED imaging resolved well-separated subdiffraction limited clusters for each protein. We quantified the size of each cluster to have a mean value of 88±48 nm and 124±43 nm, for CNG and ACIII, respectively. STED imaging showed separated clusters that were not resolvable in confocal images.

  7. Caracterización morfoanatómica comparativa entre Aloe vera (L. Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. y Aloe ciliaris Haw. (Aloeaceae

    Directory of Open Access Journals (Sweden)

    Stella Maris Carpano

    2009-03-01

    Full Text Available Aloe vera (L. Burm. F. (= Aloe barbadensis Miller conhecida como "a planta da imortalidade" no antigo Egito é utilizada em fitoterapia como humectante, antibacteriana, antifúngica, antiviral e antioxidante. Com a finalidade de contribuir para identificação da droga vegetal, foram realizados estudos morfoanatômicos da folha. A utilização de plantas com características terapêuticas reconhecidas determina que, por analogia, popularmente sejam utilizadas com a mesma finalidade outras espécies do mesmo gênero. Isto é o que ocorre com três espécies de Aloe spp., que se cultivam ou crescem acidentalmente na Argentina: A. arborescens Mill., A. saponaria Haw. e A. ciliaris Haw. (Aloaceae. Estabeleceram-se os caracteres morfoanatômicos de diagnóstico por microscopia óptica e microscopia eletrônica de varredura (apresentam-se desenhos e fotomicrografias dos mesmos. Obtiveram-se valores numéricos dos elementos histológicos de diagnóstico: magnitudes lineares e proporcionais. Realizaram-se reações histoquímicas de identificação dos princípios ativos para sua localização "in situ".

  8. Control of ventricular ciliary beating by the Melanin Concentrating Hormone-expressing neurons of the lateral hypothalamus : a functional imaging survey.

    Directory of Open Access Journals (Sweden)

    Gregory eConductier

    2013-11-01

    Full Text Available The cyclic peptide Melanin-Concentrating Hormone (MCH is known to control a large number of brain functions in mammals such as food intake and metabolism, stress response, anxiety, sleep/wake cycle, memory and reward. Based on neuroanatomical and electrophysiological studies these functions were attributed to neuronal circuits expressing MCHR1, the single MCH receptor in rodents. In complement to our recently published work (Conductier et al. 2013 we provided here new data regarding the action of MCH on ependymocytes in the mouse brain. First, we establish that MCHR1 mRNA is expressed in the ependymal cells of the third ventricle epithelium. Second, we demonstated a tonic control of MCH-expressing neurons on ependymal cilia beat frequency using in vitro optogenics. Finally, we performed in vivo measurements of CSF flow using fluorescent micro-beads in wild-type and MCHR1 knockout mice. Collectively, our results demonstrated that MCH-expressing neurons modulate ciliary beating of ependymal cells at the third ventricle and could contribute to maintain cerebro-spinal fluid homeostasis.

  9. Primary ciliary dyskinesia in the paediatric population: range and severity of radiological findings in a cohort of patients receiving tertiary care

    Energy Technology Data Exchange (ETDEWEB)

    Jain, K. [Department of Radiology, Royal Brompton and Harefield NHS Trust, London (United Kingdom); Padley, S.P.G. [Department of Radiology, Royal Brompton and Harefield NHS Trust, London (United Kingdom)], E-mail: s.padley@ic.ac.uk; Goldstraw, E.J.; Kidd, S.J. [Department of Radiology, Royal Brompton and Harefield NHS Trust, London (United Kingdom); Hogg, C.; Biggart, E.; Bush, A. [Department of Paediatric Respiratory Medicine, Royal Brompton and Harefield NHS Trust, London (United Kingdom)

    2007-10-15

    Aim: To investigate the clinical range and severity of radiological findings in a cohort of patients with primary ciliary dyskinesia (PCD) receiving tertiary care. Materials and methods: The case notes and clinical test results of 89 children attending the paediatric respiratory disease clinic at our institution were retrospectively analysed. Demographic details including age at diagnosis and common presenting signs and symptoms were studied. Results of chest radiographs, microscopy, and high-resolution computed tomography (HRCT) for quantification of lung damage were analysed. Results: In a cohort of 89 children with PCD, a presentation chest radiograph was available in 62% of patients (n = 55), with all but one demonstrating changes of bronchial wall thickening. HRCT of the lungs, available in 26 patients, were scored using the system described by Brody et al. analysing five specific features of lung disease, including bronchiectasis, mucus plugging, peribronchial thickening, parenchymal changes of consolidation, and ground-glass density, and focal air-trapping in each lobe. Peribronchial thickening was observed using HRCT in 25 patients, while 20 patients had bronchiectasis. Severity scores were highest for the middle and the lingular lobes. Conclusion: The radiographic findings of the largest reported cohort of patients with PCD are presented, with associated clinical findings. Dextrocardia remains the commonest finding on chest radiography. HRCT demonstrates peribronchial thickening and bronchiectasis, which is most marked in the lower zones. Radiological scoring techniques developed for assessment of cystic fibrosis can also be applied for the assessment of disease severity in this patient population.

  10. From a single whole exome read to notions of clinical screening: primary ciliary dyskinesia and RSPH9 p.Lys268del in the Arabian Peninsula.

    Science.gov (United States)

    Alsaadi, Muslim M; Gaunt, Tom R; Boustred, Christopher R; Guthrie, Philip A I; Liu, Xuan; Lenzi, Luca; Rainbow, Lucille; Hall, Neil; Alharbi, Khalid K; Day, Ian N M

    2012-05-01

    Primary ciliary dyskinesia (PCD) is a genetic disorder, usually autosomal recessive, causing early respiratory disease and later subfertility. Whole exome sequencing may enable efficient analysis for locus heterogeneous disorders such as PCD. We whole-exome-sequenced one consanguineous Saudi Arabian with clinically diagnosed PCD and normal laterality, to attempt ab initio molecular diagnosis. We reviewed 13 known PCD genes and potentially autozygous regions (extended homozygosity) for homozygous exon deletions, non-dbSNP codon, splice-site base variants or small indels. Homozygous non-dbSNP changes were also reviewed exome-wide. One single molecular read representing RSPH9 p.Lys268del was observed, with no wild-type reads, and a notable deficiency of mapped reads at this location. Among all observations, RSPH9 was the strongest candidate for causality. Searching unmapped reads revealed seven more mutant reads. Direct assay for p.Lys268del (MboII digest) confirmed homozygosity in the affected individual, then confirmed homozygosity in three siblings with bronchiectasis. Our finding in southwest Saudi Arabia indicates that p.Lys268del, previously observed in two Bedouin families (Israel, UAE), is geographically widespread in the Arabian Peninsula. Analogous with cystic fibrosis CFTR p.Phe508del, screening for RSPH9 p.Lys268del (which lacks sentinel dextrocardia) in those at risk would help in early diagnosis, tailored clinical management, genetic counselling and primary prevention.

  11. Cytokine-Like Factor 1, an Essential Facilitator of Cardiotrophin-Like Cytokine:Ciliary Neurotrophic Factor Receptor α Signaling and sorLA-Mediated Turnover.

    Science.gov (United States)

    Larsen, Jakob Vejby; Kristensen, Anders Mejer; Pallesen, Lone Tjener; Bauer, Johannes; Vægter, Christian Bjerggaard; Nielsen, Morten Schallburg; Madsen, Peder; Petersen, Claus Munck

    2016-04-01

    Cardiotrophin-like cytokine:cytokine-like factor-1 (CLC:CLF-1) is a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development. Mice lacking CLC:CLF-1 die soon after birth due to a suckling defect and show reduced numbers of motor neurons. Humans carrying mutations in CLC:CLF-1 develop similar disorders, known as Sohar-Crisponi or cold-induced sweating syndrome, and have a high risk of early death. It is well known that CLC binds the ciliary neurotrophic factor receptor α (CNTFRα) and is a prerequisite for signaling through the gp130/leukemia inhibitory factor receptor β (LIFRβ) heterodimer, whereas CLF-1 serves to promote the cellular release of CLC. However, the precise role of CLF-1 is unclear. Here, we report that CLF-1, based on its binding site for CLC and on two additional and independent sites for CNTFRα and sorLA, is a key player in CLC and CNTFRα signaling and turnover. The site for CNTFRα enables CLF-1 to promote CLC:CNTFRα complex formation and signaling. The second site establishes a link between the endocytic receptor sorLA and the tripartite CLC:CLF-1:CNTFRα complex and allows sorLA to downregulate the CNTFRα pool in stimulated cells. Finally, sorLA may bind and concentrate the tripartite soluble CLC:CLF-1:CNTFRα complex on cell membranes and thus facilitate its signaling through gp130/LIFRβ.

  12. Foxg1-Cre Mediated Lrp2 Inactivation in the Developing Mouse Neural Retina, Ciliary and Retinal Pigment Epithelia Models Congenital High Myopia.

    Directory of Open Access Journals (Sweden)

    Olivier Cases

    Full Text Available Myopia is a common ocular disorder generally due to increased axial length of the eye-globe. Its extreme form high myopia (HM is a multifactorial disease leading to retinal and scleral damage, visual impairment or loss and is an important health issue. Mutations in the endocytic receptor LRP2 gene result in Donnai-Barrow (DBS and Stickler syndromes, both characterized by HM. To clearly establish the link between Lrp2 and congenital HM we inactivated Lrp2 in the mouse forebrain including the neural retina and the retinal and ciliary pigment epithelia. High resolution in vivo MRI imaging and ophthalmological analyses showed that the adult Lrp2-deficient eyes were 40% longer than the control ones mainly due to an excessive elongation of the vitreal chamber. They had an apparently normal intraocular pressure and developed chorioretinal atrophy and posterior scleral staphyloma features reminiscent of human myopic retinopathy. Immunomorphological and ultrastructural analyses showed that increased eye lengthening was first observed by post-natal day 5 (P5 and that it was accompanied by a rapid decrease of the bipolar, photoreceptor and retinal ganglion cells, and eventually the optic nerve axons. It was followed by scleral thinning and collagen fiber disorganization, essentially in the posterior pole. We conclude that the function of LRP2 in the ocular tissues is necessary for normal eye growth and that the Lrp2-deficient eyes provide a unique tool to further study human HM.

  13. Localization and expression of ciliary neurotrophic factor (CNTF) in postmortem sciatic nerve from patients with motor neuron disease and diabetic neuropathy

    Energy Technology Data Exchange (ETDEWEB)

    Lee, D.A. [Univ. Medical Center, New Orleans, LA (United States); Gross, L.; Wittrock, D.A.; Windebank, A.J. [Mayo Clinic, Rochester, MN (United States)

    1996-08-01

    Ciliary neurotrophic factor (CNTF) is thought to play an important role in the maintenance of the mature motor system. The factor is found most abundantly in myelinating Schwann cells in the adult sciatic nerve. Lack of neuronal growth factors has been proposed as one possible etiology of amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Growth factor replacement therapies are currently being evaluated as a treatment for motor neuron disease. In this report we determined whether the expression of CNTF in sciatic nerve differed in patients with motor neuron disease compared to controls or patients with another form of axonopathy. We identified 8 patients (7 with ALS and 1 with SMA) with motor neuron disease and 6 patients with diabetic motor neuropathy who had autopsy material available. Immunoperoxidase staining showed reduced CNTF expression in nerves of patients with motor neuron disease but not in patients with diabetic motor neuropathy. Decreased CNTF appears be associated with primary motor neuron disease rather than a generalized process of axon loss. This result supports suggestions that CNTF deficiency may be an important factor in the development of motor neuron disease. 20 refs., 4 figs., 1 tab.

  14. Zebrafish Ciliopathy Screen Plus Human Mutational Analysis Identifies C21orf59 and CCDC65 Defects as Causing Primary Ciliary Dyskinesia

    Science.gov (United States)

    Austin-Tse, Christina; Halbritter, Jan; Zariwala, Maimoona A.; Gilberti, Renée M.; Gee, Heon Yung; Hellman, Nathan; Pathak, Narendra; Liu, Yan; Panizzi, Jennifer R.; Patel-King, Ramila S.; Tritschler, Douglas; Bower, Raqual; O’Toole, Eileen; Porath, Jonathan D.; Hurd, Toby W.; Chaki, Moumita; Diaz, Katrina A.; Kohl, Stefan; Lovric, Svjetlana; Hwang, Daw-Yang; Braun, Daniela A.; Schueler, Markus; Airik, Rannar; Otto, Edgar A.; Leigh, Margaret W.; Noone, Peadar G.; Carson, Johnny L.; Davis, Stephanie D.; Pittman, Jessica E.; Ferkol, Thomas W.; Atkinson, Jeffry J.; Olivier, Kenneth N.; Sagel, Scott D.; Dell, Sharon D.; Rosenfeld, Margaret; Milla, Carlos E.; Loges, Niki T.; Omran, Heymut; Porter, Mary E.; King, Stephen M.; Knowles, Michael R.; Drummond, Iain A.; Hildebrandt, Friedhelm

    2013-01-01

    Primary ciliary dyskinesia (PCD) is caused when defects of motile cilia lead to chronic airway infections, male infertility, and situs abnormalities. Multiple causative PCD mutations account for only 65% of cases, suggesting that many genes essential for cilia function remain to be discovered. By using zebrafish morpholino knockdown of PCD candidate genes as an in vivo screening platform, we identified c21orf59, ccdc65, and c15orf26 as critical for cilia motility. c21orf59 and c15orf26 knockdown in zebrafish and planaria blocked outer dynein arm assembly, and ccdc65 knockdown altered cilia beat pattern. Biochemical analysis in Chlamydomonas revealed that the C21orf59 ortholog FBB18 is a flagellar matrix protein that accumulates specifically when cilia motility is impaired. The Chlamydomonas ida6 mutant identifies CCDC65/FAP250 as an essential component of the nexin-dynein regulatory complex. Analysis of 295 individuals with PCD identified recessive truncating mutations of C21orf59 in four families and CCDC65 in two families. Similar to findings in zebrafish and planaria, mutations in C21orf59 caused loss of both outer and inner dynein arm components. Our results characterize two genes associated with PCD-causing mutations and elucidate two distinct mechanisms critical for motile cilia function: dynein arm assembly for C21orf59 and assembly of the nexin-dynein regulatory complex for CCDC65. PMID:24094744

  15. [Perspectives on body: embodiment and body image].

    Science.gov (United States)

    Chang, Shiow-Ru; Chao, Yu-Mei Yu

    2007-06-01

    "Body" is a basic concept of both the natural and human sciences. This extensive review of the literature explores the various philosophical approaches to the body, including empiricism, idealism, existentialism and phenomenology, as well as the relationship between body and mind. Embodiment and body image are the two main concepts of body addressed in this article. Merleau-Ponty's perspective on embodiment, an important new area of theory development, emphasizes that embodiment research must focus on life experiences, such as the study of body image. Using Schilder's framework of psychosocialology, this article provides a comprehensive understanding of the concept of body image and women's perspectives on the "body" in both Western culture and Eastern cultures. Body size and shape significantly influence the self-image of women. Body image is something that develops and changes throughout one's life span and is continually being constructed, destructed, and reconstructed. Personal body image has important psychological effects on the individual, especially women. This integrative review can make a significant contribution to knowledge in this area and, consequently, to related practice and research.

  16. Lewy Body Disease

    Science.gov (United States)

    Lewy body disease is one of the most common causes of dementia in the elderly. Dementia is the loss of mental ... to affect normal activities and relationships. Lewy body disease happens when abnormal structures, called Lewy bodies, build ...

  17. Body & Lifestyle Changes

    Science.gov (United States)

    ... Close X Home > Pregnancy > Body & lifestyle changes Body & lifestyle changes E-mail to a friend Please fill ... between pregnancies Nutrition, weight & fitness Prenatal care Body & lifestyle changes Is it safe? Labor & birth Postpartum care ...

  18. Inclusion Body Myositis

    Science.gov (United States)

    ... Awards Enhancing Diversity Find People About NINDS NINDS Inclusion Body Myositis Information Page Table of Contents (click ... and Information Additional resources from MedlinePlus What is Inclusion Body Myositis? Inclusion body myositis (IBM) is one ...

  19. Foreign body orbital cyst

    DEFF Research Database (Denmark)

    Yazdanfard, Younes; Heegard, Steffen; Fledelius, Hans C.;

    2001-01-01

    Ophthalmology, penetrating orbital injury, orbital foreign body, ultrasound, computed tomography (CT), histology......Ophthalmology, penetrating orbital injury, orbital foreign body, ultrasound, computed tomography (CT), histology...

  20. Body Matters: Narratives of the Body

    OpenAIRE

    Asandi, Iren; Filipovska, Kalina; Neault, Megan; Olsen, Sara Høier

    2014-01-01

    This project engages the notion of the subjective body in a pasture of social constructions in order to gather an understanding of the narratives created by women about their bodies in relation to cosmetic surgery. The empirical data for this project comes from our virtual ethnographic research on the various forums from the MyLooks website. Moreover, perspectives regarding the body, beauty ideals and theoretical positions from Kathryn Morgan and Kathy Davis fill out the structure of the proj...

  1. [Multifaceted body. 3. The contextualised body].

    Science.gov (United States)

    Bourquin, C; Wykretowicz, H; Saraga, M; Stiefel, F

    2015-02-11

    The human body is the object upon which medicine is acting, but also lived reality, image, symbol, representation and the object of elaboration and theory. All these elements which constitute the body influence the way medicine is treating it. In this series of three articles, we address the human body from various perspectives: medical (1), phenomenological (2), psychosomatic and socio-anthropological (3). This third and last article focuses on the psychosomatic and socio-anthropological facets of the body and their contribution to its understanding. PMID:25895216

  2. Determination of Star Bodies from -Centroid Bodies

    Indian Academy of Sciences (India)

    Lujun Guo; Gangsong Leng

    2013-11-01

    In this paper, we prove that an origin-symmetric star body is uniquely determined by its -centroid body. Furthermore, using spherical harmonics, we establish a result for non-symmetric star bodies. As an application, we show that there is a unique member of $_p\\langle K \\rangle$ characterized by having larger volume than any other member, for all real ≥ 1 that are not even natural numbers, where $_p\\langle K \\rangle$ denotes the -centroid equivalence class of the star body .

  3. [Multifaceted body. 3. The contextualised body].

    Science.gov (United States)

    Bourquin, C; Wykretowicz, H; Saraga, M; Stiefel, F

    2015-02-11

    The human body is the object upon which medicine is acting, but also lived reality, image, symbol, representation and the object of elaboration and theory. All these elements which constitute the body influence the way medicine is treating it. In this series of three articles, we address the human body from various perspectives: medical (1), phenomenological (2), psychosomatic and socio-anthropological (3). This third and last article focuses on the psychosomatic and socio-anthropological facets of the body and their contribution to its understanding.

  4. Media and Body Image

    Science.gov (United States)

    ... Management Education & Events Advocacy For Patients About ACOG Media and Body Image Home For Patients Search FAQs Media and Body ... and Body Image TFAQ002, June 2016 PDF Format Media and Body Image Especially For Teens How can the media make ...

  5. Adolescence and Body Image.

    Science.gov (United States)

    Weinshenker, Naomi

    2002-01-01

    Discusses body image among adolescents, explaining that today's adolescents are more prone to body image distortions and dissatisfaction than ever and examining the historical context; how self-image develops; normative discontent; body image distortions; body dysmorphic disorder (BDD); vulnerability of boys (muscle dysmorphia); who is at risk;…

  6. Reinforced Airfoil Shaped Body

    DEFF Research Database (Denmark)

    2011-01-01

    The present invention relates to an airfoil shaped body with a leading edge and a trailing edge extending along the longitudinal extension of the body and defining a profile chord, the airfoil shaped body comprising an airfoil shaped facing that forms the outer surface of the airfoil shaped body...

  7. General -Harmonic Blaschke Bodies

    Indian Academy of Sciences (India)

    Yibin Feng; Weidong Wang

    2014-02-01

    Lutwak introduced the harmonic Blaschke combination and the harmonic Blaschke body of a star body. Further, Feng and Wang introduced the concept of the -harmonic Blaschke body of a star body. In this paper, we define the notion of general -harmonic Blaschke bodies and establish some of its properties. In particular, we obtain the extreme values concerning the volume and the -dual geominimal surface area of this new notion.

  8. Lung disease assessment in primary ciliary dyskinesia: a comparison between chest high-field magnetic resonance imaging and high-resolution computed tomography findings

    Directory of Open Access Journals (Sweden)

    Iacotucci Paola

    2009-08-01

    Full Text Available Abstract Background Primary ciliary dyskinesia (PCD is associated with pulmonary involvement that requires periodical assessment. Chest high-resolution computed tomography (HRCT has become the method of choice to evaluate chronic lung disease, but entails exposure to ionizing radiation. Magnetic resonance imaging (MRI has been proposed as a potential radiation-free technique in several chest disorders. Aim of our study is to evaluate whether high-field MRI is as effective as HRCT in identifying PCD pulmonary abnormalities. We also analyzed the relationships between the severity and extension of lung disease, and functional data. Methods Thirteen PCD patients (8 children/5 adults; median age, 15.2 yrs underwent chest HRCT and high-field 3T MRI, spirometry, and deep throat or sputum culture. Images were scored using a modified version of the Helbich system. Results HRCT and MRI total scores were 12 (range, 6–20 and 12 (range, 5–17, respectively. Agreement between HRCT and MRI scores was good or excellent (r > 0.8. HRCT and MRI total scores were significantly related to forced vital capacity (r = -0.5, p = 0.05; and r = -0.7, p = 0.009, respectively and forced expiratory volume at 1 second (r = -0.6, p = 0.03; and r = -0.7, p = 0.009, respectively. Conclusion Chest high-field 3T MRI appears to be as effective as HRCT in assessing the extent and severity of lung abnormalities in PCD. MRI scores might be used for longitudinal assessment and be an outcome surrogate in future studies.

  9. 离体猪眼睫状体上皮电参数测量%The in Vitro Electrical Parameters of the Isolated Porcine Ciliary Epithelium

    Institute of Scientific and Technical Information of China (English)

    凌运兰; 杜嗣河; 林明楷; 卓业鸿; 侯飞; 江志荣; 葛坚

    2006-01-01

    目的:测量离体猪眼跨睫状体上皮(ciliary epithelium,CE)电参数,为人眼睫状体研究提供依据.方法:应用改良的Ussing-chamber连续灌注系统对离体的猪眼睫状体上皮电参数:电阻(resistance,R)、电位差(potential difference,PD)及短路电流(short-circuit current,SCC)进行测量,并观察哇巴因(ouabain)对电参数的影响.结果:猪眼跨睫状体上皮房水侧PD为:(-0.6±0.1)mV,且始终保持负性.SCC及R分别为:(-10.9±1.2)μA/cm2及(54.6±1.6)Ωcm2.0.1mM ouabain应用于房水侧PD及SCC表现为先超极化再去极化,即先是刺激作用,然后是抑制作用.结论:猪眼跨睫状体上皮房水侧PD为负性;Na+,K+-ATP酶存在于猪眼色素上皮(pigmented epithelium,PE)和非色素上皮(non-pigmented epithelium,NPE)细胞上,且对维持其睫状体上皮房水侧电极性为负性起重要的作用.

  10. Dose-dependent neuroprotective effect of ciliary neurotrophic factor delivered via tetracycline-regulated lentiviral vectors in the quinolinic acid rat model of Huntington's disease.

    Science.gov (United States)

    Régulier, E; Pereira de Almeida, L; Sommer, B; Aebischer, P; Déglon, N

    2002-11-01

    The ability to regulate gene expression constitutes a prerequisite for the development of gene therapy strategies aimed at the treatment of neurologic disorders. In the present work, we used tetracycline (Tet)-regulated lentiviral vectors to investigate the dose-dependent neuroprotective effect of human ciliary neurotrophic factor (CNTF) in the quinolinic acid (QA) model of Huntington's disease (HD). The Tet system was split in two lentiviruses, the first one containing the CNTF or green fluorescent protein (GFP) cDNAs under the control of the Tet-response element (TRE) and a second vector encoding the transactivator (tTA). Preliminary coinfection study demonstrated that 63.8% +/- 2.0% of infected cells contain at least two viral copies. Adult rats were then injected with CNTF- and GFP-expressing viral vectors followed 3 weeks later by an intrastriatal administration of QA. A significant reduction of apomorphine-induced rotations was observed in the CNTF-on group. In contrast, GFP-treated animals or CNTF-off rats displayed an ipsilateral turning behavior in response to apomorphine. A selective sparing of DARPP-32-, choline acetyltransferase (ChAT)-, and NADPH-d-positive neurons was observed in the striatum of CNTF-on rats compared to GFP animals and CNTF-off group. Enzyme-linked immunosorbent assay (ELISA) performed on striatal samples of rats sacrificed at the same time point indicated that this neuroprotective effect was associated with the production of 15.5 +/- 4.7 ng CNTF per milligram of protein whereas the residual CNTF expression in the off state (0.54 +/- 0.02 ng/mg of protein) was not sufficient to protect against QA toxicity. These results establish the proof of principle of neurotrophic factor dosing for neurodegenerative diseases and demonstrate the feasibility of lentiviral-mediated tetracycline-regulated gene transfer in the brain. PMID:12427308

  11. A model of flagellar and ciliary functioning which uses the forces transverse to the axoneme as the regulator of dynein activation.

    Science.gov (United States)

    Lindemann, C B

    1994-01-01

    Ciliary and flagellar motion is driven by the dynein-tubulin interaction between adjacent doublets of the axoneme, and the resulting sliding displacements are converted into axonemal bends that are propagated. When the axoneme is bent in the normal beating plane, force develops across the axoneme in the plane of the bend. This transverse force (t-force) has maximal effect on the interdoublet spacing of outer doublets 2-4 on one side of the axoneme and doublets 7-9 on the opposite side. Episodes of sliding originates as the t-force brings these doublets into closer proximity (allowing dynein bridges to form) and are terminated when these doublets are separated from each other by the t-force. A second factor, the adhesive force of the dynein-tubulin attachments (bridges), also acts to pull neighboring doublets closer together. This force resists termination of a sliding episode once initiated, and acts locally to give the population of dynein bridges a type of excitability. In other words, as bridges form, the probability of nearby bridges attaching is increased by a positive feedback exerted through the interdoublet spacing. A conceptual working hypothesis explaining the behavior of cilia and flagella is proposed based on the above concepts. Additionally, the feasibility of this proposed mechanism is demonstrated using a computer simulation. The simulation uses a Monte Carlo-type algorithm for dynein attachment and adhesive force, together with a geometric evaluation of the t-force on the key microtubule pairs. This model successfully develops spontaneous oscillations from any starting configuration (including a straight position). It is compatible with the physical dimensions, mechanical properties and bridge forces measured in real cilia and flagella. In operation, it exhibits many of the observed actions of cilia and flagella, most notably wave propagation and the ability to produce both cilia-like and flagella-like waveforms. PMID:7820864

  12. Discinesia ciliar primária: quando o pediatra deve suspeitar e como diagnosticar? Primary ciliary dyskinesia: when the pediatrician must suspect and how to do the diagnosis?

    Directory of Open Access Journals (Sweden)

    Mary Anne K. Olm

    2007-12-01

    Full Text Available OBJETIVO: Revisar a discinesia ciliar primária (DCP quanto aos seus aspectos ultra-estruturais, discriminar os defeitos ciliares primários dos secundários, descrever o quadro clínico, os testes laboratoriais de triagem e de diagnóstico disponíveis, bem como seu manejo clínico. FONTE DE DADOS: Pesquisa nas bases de dados Medline, Lilacs e SciELO, no período de 1980 a 2007. SÍNTESE DOS DADOS: A DCP é uma doença autossômica recessiva que compromete a estrutura e/ou a função ciliar e, conseqüentemente, o transporte mucociliar. As manifestações clínicas envolvem o trato respiratório superior e inferior, com infecções recorrentes do ouvido médio, seios paranasais e pulmonares, que podem evoluir para bronquiectasias. Outras manifestações incluem situs inversus totalis e infertilidade masculina. O diagnóstico deve ser suspeitado pelos pediatras em várias situações: recém-nascidos de termo com desconforto respiratório sem causa aparente; neonatos portadores de dextrocardia; lactentes com tosse persistente e/ou infecções otorrinolaringológicas de repetição, excluindo-se as imunodeficiências e a fibrose cística; crianças com asma atípica e as com bronquiectasias sem causa definida. Os testes de triagem diagnóstica são os da sacarina e do óxido nítrico nasal. As avaliações do defeito ultra-estrutural e funcional exigem análise por microscopia eletrônica e da freqüência e formato da onda de batimento ciliar. CONCLUSÕES: A DCP, apesar da baixa prevalência, é pouco diagnosticada pelas dificuldades de estabelecer o diagnóstico definitivo do defeito ciliar devido à complexidade da investigação laboratorial e pela falta de reconhecimento da doença pelos médicos. A suspeita clínica e o diagnóstico precoce são fundamentais para reduzir a morbidade e prevenir o desenvolvimento de complicações.OBJECTIVE: To review primary ciliary dyskinesia (PCD and its ultrastructural aspects, to differentiate primary

  13. 药物性睫状环阻滞性青光眼的治疗%Surgery for drug causing ciliary-block glaucoma

    Institute of Scientific and Technical Information of China (English)

    程宏松; 张有亭; 胡宏阁; 赵晏黎

    2010-01-01

    目的 分析药物性睫状环阻滞性青光眼的临床特点,总结其治疗方法和治疗效果.方法 对2001年4月至2008年12月在本院治疗的此类型睫状环阻滞性青光眼的临床资料进行总结、回顾性分析.结果 ①眼压:入院时的眼压43.38~81.78 mm Hg,平均为(51.66±5.46)mm Hg;经治疗10~14 d后,所有患者眼压均控制在25 mm Hg以内.随访12月后,2例长期局部点眼药眼压一直维持在20 mm Hg左右,其他采用手术治疗的10例患者,有6例不用降眼压药物,眼压控制在20 mm Hg以内;3例眼压在21~25 mm Hg,给予局部点β-受体阻滞剂,眼压控制在20 mm Hg以内,1例眼压失控而再次手术,最终失明;②视力:治疗前12例最佳矫正视力为1 m光感至0.3,治疗后最佳矫正视力0.1~0.8.治疗前后视力比较,差异有显著性(P<0.01).结论 这类睫状环阻滞性青光眼都具有闭角型青光眼的解剖结构,用毛果芸香碱眼压增高,用眼压下降,积极治疗可恢复有用视力.%Objective To study clinical characteristic of drug causingciliary-block glaucoma,to evaluate the therapeutic effect of surgery for drug causing ciliary-block glaucoma. Methods The clinical informations of 12cases( 12 eyes) of drug causing ciliary-block glaucoma diagnosed in our hospital between April 2001 and December 2008 were evaluated, vistaed summary. Results ①Intraocular pressure (IOP) :Being hospitalized,the IOP of patients was 43.38 ~ 81.78 mm Hg, the mean was (51.66 ± 5.46 ) mm Hg; After treating for 10 ~ 14 days, the IOP of 12 patients were controlled below 25 mm Hg. Following up 12 months, two patients kept their IOP at about 20 mm Hg by using eye-drops; The other 10 patients performed an operation, the IOP of 6 patients were controlled at about 20 mm Hg without giving medicine; Three patients were 21 ~ 25 mm Hg, their IOP was controlled at 20 mm Hg after giving β-receptor block medicine. The IOP of one patient was out of control; the patient lost his

  14. Body Image and Body Dysmorphic Concerns.

    Science.gov (United States)

    Tomas-Aragones, Lucia; Marron, Servando E

    2016-08-23

    Most people would like to change something about their bodies and the way that they look, but for some it becomes an obsession. A healthy skin plays an important role in a person's physical and mental wellbeing, whereas a disfiguring appearance is associated with body image concerns. Skin diseases such as acne, psoriasis and vitiligo produce cosmetic disfigurement and patients suffering these and other visible skin conditions have an increased risk of depression, anxiety, feelings of stigmatization and self-harm ideation. Body image affects our emotions, thoughts, and behaviours in everyday life, but, above all, it influences our relationships. Furthermore, it has the potential to influence our quality of life. Promotion of positive body image is highly recommended, as it is important in improving people's quality of life, physical health, and health-related behaviors. Dermatologists have a key role in identifying body image concerns and offering patients possible treatment options. PMID:27283435

  15. Research progress of airway ciliary function and related respiratory diseases%气道纤毛功能及相关呼吸系统疾病研究进展

    Institute of Scientific and Technical Information of China (English)

    冯宇; 李庆云; 刘嘉琳; 万欢英

    2010-01-01

    The normal mucociliary clearance system (MCC) requires not only mucus with optimum of viscoelastic and thickness, but also enough cilia with structural integrity and sufficient cilia beat frequency(CBF). Abnormality of ciliary structure and function, CBF or surface properties of mucus was found in several respiratory diseases, such as chronic obstructive pulmonary diseases, primary ciliary dyskinesia,cystic fibrosis,etc. Which implies the MCC should be new area for research on treatment of respiratory diseases. The review will focus on the research progress of airway ciliary function and related respiratory diseases.%正常的黏液纤毛清除功能要求黏液具有最佳的黏弹性和厚度,而足够数量、结构完整和一定摆动频率的纤毛则更重要.由于慢性阻塞性肺疾病,原发性纤毛运动障碍、囊性纤维化等多种疾病均存在纤毛结构和功能异常或纤毛周围的黏液流变学特征改变,因此气道黏液纤毛清除功能成为探索相关治疗的新领域之一.本文就影响纤毛结构及运动的因素及相关的呼吸系统疾病的研究进展作综述.

  16. 睫状神经营养因子对糖尿病早期大鼠视网膜神经组织的保护作用%Protective effects of ciliary neurotrophic factor on retinal neurons in rats with early diabetes

    Institute of Scientific and Technical Information of China (English)

    梁紫岩; 张卯年

    2012-01-01

    AIM: To evaluate the protective effects of ciliary neurotrophic factor (CNTF) against retinal neurons damage in a rat model of diabetes mellitus(DM).METHODS: Forty Sprague Dawley rats (weighing 250g-280g) received an intraperitoneal injection of streptozotocin (60mg/kg). The rats were then randomly divided into two groups: the treatment (DM+CNTF) or control (DM+BSS) group. The CNTF (0.5μg/μL) or balanced salt solution (2μL) was injected into the vitreus of the rats. Apoptosis of retinal ganglion cells (RGCs) was measured and retinal ultrastructure was assessed 4 and 12 weeks after establishing the model and performing the injections.RESULTS: No difference in blood glucose or body weight was noted between the two groups. There were significantly fewer apoptotic RGCs in DM+CNTF group (8.56±1.22, P0.05).12wk时TUNEL检测DM+CNTF组大鼠神经节细胞凋亡与DM+BSS组相比减少(P<0.05).透射电镜下观察发现从4wk起两组大鼠视网膜神经组织出现细胞凋亡的改变,经CNTF治疗细胞凋亡改变有所减轻,表现为外节膜盘间隙减小,感光细胞水肿减轻及核染色质浓集减轻等.结论:CNTF对DM+CNTF组和DM+BSS组大鼠的体重及血糖无明显影响.CNTF治疗组结果显示对本实验糖尿病大鼠视网膜神经节细胞及感光细胞有一定保护作用.

  17. Abstract: Body Work

    DEFF Research Database (Denmark)

    Otto, Lene

    2012-01-01

    . The British sociologist Julia Twigg has introduced and explored the term `bodywork', most recently in Body Work in Health and Social Care - Critical Themes, New Agendas (2011). She extends the term body work from applying to the work that individuals undertake on their own bodies, often as part of regimens......This panel will explore the usefulness of the term ‘body work’ in cultural history. Body work is understood as work focusing on the bodies of others as component in a range of occupations in health and social care, as well as in unpaid work in the family. How can the notion of body work inform...... cultural history of health and illness whether through a micro-social focus on the intercorporeal aspects of work in health and social care, or through clarifying our understanding of the times and spaces of work, or through highlighting the relationship between mundane body work and global processes...

  18. Lewy Body Dementia Diagnosis

    Science.gov (United States)

    ... Diagnosis Symptoms Treatment Options Help end Lewy body dementia now! Donate Diagnosis An experienced clinician within the ... an experienced diagnostic team skilled in Lewy body dementia. A thorough dementia diagnostic evaluation includes physical and ...

  19. Lewy Body Dementia Research

    Science.gov (United States)

    ... Research Abstracts Clinical Trials Help end Lewy body dementia now! Donate Research Links Treating Psychosis in Parkinson’s ... The use of antipsychotic medications in Lewy body dementias is a known challenge. Are the medications helpful ...

  20. About Body Water

    Science.gov (United States)

    ... Insulin Delivery Additional Content Medical News About Body Water By James L. Lewis, III, MD NOTE: This ... Version. DOCTORS: Click here for the Professional Version Water Balance About Body Water Dehydration Overhydration Water accounts ...

  1. Body mass index

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/007196.htm Body mass index To use the sharing features on this ... your height is to figure out your body mass index (BMI). You and your health care provider ...

  2. Zooplankton body composition

    DEFF Research Database (Denmark)

    Kiørboe, Thomas

    2013-01-01

    I compiled literature on zooplankton body composition, from protozoans to gelatinous plankton, and report allometric relations and average body composition. Zooplankton segregate into gelatinous and non-gelatinous forms, with few intermediate taxa (chaetognaths, polychaetes, and pteropods). In most...... groups body composition is size independent. Exceptions are protozoans, chaetognaths, and pteropods, where larger individuals become increasingly watery. I speculate about the dichotomy in body composition and argue that differences in feeding mechanisms and predator avoidance strategies favor either a...

  3. Forage supply in thinned Caatinga enriched with buffel grass (Cenchrus ciliaris L. grazed by goats and sheep - doi: 10.4025/actascianimsci.v34i2.12548

    Directory of Open Access Journals (Sweden)

    Diogo da Costa Soares

    2012-03-01

    Full Text Available Forage supply from herbs was assessed in a thinned Caatinga enriched with buffel grass (Cenchrus ciliaris L. exposed to goat and sheep grazing. The 2.4 ha experimental area, located at the Experimental Station of the Federal University of Campina Grande, in Santa Terezinha, Paraíba State, Brazil, was divided into four 0.6 ha paddocks, which were further subdivided into two 0.3 ha experimental plots. Twelve F1 (Boer x SRD goats and 12 Santa Inês sheep were divided in four groups of six animals of the same species. The herbaceous vegetation was separated into buffel grass (Cenchrus ciliaris L., dicotyledons and other grass species. Treatments were randomized to plots according to a completely random design with two treatments, four replications, with measures repeated in time (July/1, Aug/1, Sept/1 and Oct/1. Buffel grass dry mater (DM availability was higher in the sheep grazed than in the goat-grazed area. Buffel grass DM supply did not change from July to September, while dicotyledons DM supply decreased. Grazing affected availability, accumulation rate and supply of the forage produced (DM basis by dicotyledonous herbs. Buffel grass forage availability was not affected during the experimental period.

  4. Michel Foucault's bodies

    OpenAIRE

    Potte-Bonneville, Mathieu

    2012-01-01

    How is it possible for Foucault to present the body at the same time as the foundation and the result of history, as condition and horizon of the theory that takes hold of it ? One has to pay attention to the various registers in which Foucault distributes the acceptations ordinarily confused with the general notion of the body : from "my body" (as it appears in Merleau-Ponty's phenomenology) to "the body' (as it is understood by modern medicine) ; from this body as an object for positive exp...

  5. 重组人睫状神经营养因子的聚乙二醇化修饰%PEGylation of Recombinant Human Ciliary Neurotrophic Factor

    Institute of Scientific and Technical Information of China (English)

    梁凌宇; 雷清; 高雪峰; 杨军; 应莲芳; 蒋琳

    2014-01-01

    Objective: To study the PEGylation of recombinant human ciliary neurotrophic factor(rhCNTF), estab-lish the preliminary separation and purification of the polyethylene glycol-modified rhCNTF, and then to detect the biological activity of PEGylation rhCNTF. Methods: Using molecular biology techniques to obtain the mutants CN10 of rhCNTF by point mutation, then to study the conditions of PEG conjugation by design of experiment. The prod-uct of PEGylation rhCNTF were purified by molecular sieve chromatography, finally the biological activity were de-tected by ELISA and the method of weight loss in normal mouse. Results: CN10 can be successfully fixed-point modified by mPEG-MAL, the weight loss in mice experiments showed that the weight increase inhibitory rate of PEGylation CN10 were up to 50% when received an intraperitoneal injection every other day, it was equivalent to inhibitory effect of rhCNTF which injected twice a day. Conclusion: After PEG modification, the biological effect of rhCNTF was significantly prolonged.%目的:研究重组人睫状神经营养因子(rhCNTF)突变体的聚乙二醇(PEG)化修饰,对rhCNTF的PEG化产物进行初步分离纯化及相关生物活性检测。方法:采用分子生物学技术经点突变得到rhCNTF的突变体CN10,通过实验设计研究CN10的最佳PEG化条件;采用分子筛层析方式对偶联产物进行初步纯化,最后用ELISA和小鼠体重增长抑制法检测PEG化后的CN10蛋白的生物活性。结果:能运用mPEG-MAL对CN10进行定点修饰,PEG化后用Superdex 200能够分离CN10;PEG化后的CN10每2 d腹腔注射1次,对小鼠体重的增长抑制率可达50%,与rhCNTF每天注射2次的体重增长抑制作用相当。结论:CN10蛋白在PEG化修饰后,其减重效应持续时间明显延长。

  6. COMPARATIVE THERAPEUTIC EFFECTS OF VARIOUS MORPHOLOGICAL FORMS OF NASYA (NASAL ROUTE OF DRUG DELIVERY IN PRATISHYAYA (RHINOSINUSITIS WITH REFERENCE TO NASAL MUCO-CILIARY FUNCTION

    Directory of Open Access Journals (Sweden)

    Bhardwaj Atul

    2012-10-01

    lipid base Nasya probably increases the physical weight on the mucosa thus deceases the ciliary transport transiently. Pippalyadi Avpeeda nasya (aqueous base nasal drops was found to be most effective in enhancing mucociliary clearance activity.

  7. Written on the Body

    DEFF Research Database (Denmark)

    Christiansen, Steen Ledet

    may choose to alter how we are perceived and to at least some extent control the discontent we may project onto our own body. Through body modification, we can alter the impression of our personality and express a cultural solidarity, as Chris Rojek points out. Tattoos, piercings and other body...... as a semiotic strategy to negotiate and navigate cultural borders. Suicidegirls.com is a website which is both an online community, but also a softcore pin-up site, where the models feature extensive body modifications in the form of tattoos and piercings. The website promotes a democratic approach to the photo...... Butler's account of subversive bodily acts, the pin-up shoots of the Suicide Girls mount a critique of a culture's view of the body as a natural entity. Cultural borders are crossed, as the bodies of the Suicide Girls embed ink into their bodies in the form of tattoos, and gender is played...

  8. Nucleation of nuclear bodies.

    Science.gov (United States)

    Dundr, Miroslav

    2013-01-01

    The nucleus is a complex organelle containing numerous highly dynamic, structurally stable domains and bodies, harboring functions that have only begun to be defined. However, the molecular mechanisms for their formation are still poorly understood. Recently it has been shown that a nuclear body can form de novo by self-organization. But little is known regarding what triggers the formation of a nuclear body and how subsequent assembly steps are orchestrated. Nuclear bodies are frequently associated with specific active gene loci that directly contribute to their formation. Both coding and noncoding RNAs can initiate the assembly of nuclear bodies with which they are physiologically associated. Thus, the formation of nuclear bodies occurs via recruitment and consequent accumulation of resident proteins in the nuclear bodies by nucleating RNA acting as a seeder. In this chapter I describe how to set up an experimental cell system to probe de novo biogenesis of a nuclear body by nucleating RNA and nuclear body components tethered on chromatin. PMID:23980018

  9. The Mallory body

    DEFF Research Database (Denmark)

    Jensen, K; Gluud, C

    1994-01-01

    To aid understanding of markers of disease and predictors of outcome in alcohol-exposed systems, we undertook a literature survey of more than 700 articles to view the morphological characteristics and the clinical and experimental epidemiology of the Mallory body. Mallory bodies are filaments...... suggest a hit-and-run effect of alcohol, whereas other chronic liver diseases show evidence of gradual increase in prevalence of Mallory bodies with severity of hepatic pathology. Mallory bodies in cirrhosis do not imply alcoholic pathogenesis. Obesity, however, is associated with alcoholism and diabetes...

  10. Resistência de populações de capim-colchão (Digitaria ciliaris aos herbicidas inibidores da Acetil Co-A Carboxilase Resistance of crab-grass (Digitaria ciliaris populations to Acetyl-Co-A Carboxylase-inhibiting herbicides

    Directory of Open Access Journals (Sweden)

    R.F. López-Ovejero

    2005-09-01

    Full Text Available O objetivo do presente trabalho foi avaliar as populações com suspeita de ocorrência de biótipos resistentes de Digitaria ciliaris aos herbicidas inibidores da AcetilCoA Carboxilase (ACCase, por meio de curvas de dose-resposta, bem como estabelecer o grau de resistência cruzada aos herbicidas cicloexanodionas (CHD e ariloxifenoxipropionatos (APP desses biótipos. O experimento foi conduzido em casa de vegetação, utilizando-se quatro populações com suspeita de resistência (R1, R2, R3 e R4 e uma população suscetível (S. O delineamento experimental adotado foi o de blocos ao acaso, com quatro repetições, sendo os tratamentos resultado da interação fatorial entre cinco populações, três herbicidas (fluazifop-p-butil, sethoxydim e tepraloxydim e oito doses de herbicidas (0C, 0,06C, 0,125C, 0,5C, 1C, 2C, 4C e 10C, em que C é a dose comercial recomendada para cada produto. Foram realizadas avaliações de porcentagem de controle aos 28 dias após a aplicação (DAA. A partir dos resultados obtidos, conclui-se que as populações R1, R2, R3 e R4 apresentaram-se como biótipos resistentes aos herbicidas inibidores da ACCase, com diferentes níveis de resistência cruzada aos herbicidas com esse mecanismo de ação.This research aimed to verify the occurrence of resistant biotypes of crab-grass (Digitaria ciliaris to Acetyl-Co-A Carboxylase (ACCase-inhibiting herbicides through dose-response curves, as well as to establish the degree of cross resistance to the CHD and APP herbicides of these biotypes. The experiment was conducted in the greenhouse and tested four suspected resistant populations (R1, R2, R3 e R4 and a susceptible one (S. The experimental design adopted was randomized complete blocks with four replications, and the treatments were the result of a factorial interaction among five populations, three herbicides (fluazifop-p-butyl, sethoxydim and tepraloxydim, and eight rates (0C, 0.06C, 0.125C, 0.5C, 1C, 2C, 4C and 10C

  11. Body-Worn Antennas for Body-Centric Wireless Communications

    DEFF Research Database (Denmark)

    Kammersgaard, Nikolaj Peter Iversen; Kvist, Søren H.; Ôzden, Sinasi ̈;

    2014-01-01

    Ear-to-ear (E2E) on-body propagation and on-body antennas for body-centric wireless communications are presented.......Ear-to-ear (E2E) on-body propagation and on-body antennas for body-centric wireless communications are presented....

  12. 白内障手术中l期人工晶状体睫状沟缝线固定术的体会%Experience of intraocular lens implantation sutured in ciliary sulcus at l phase in cataract surgery

    Institute of Scientific and Technical Information of China (English)

    吕宏伟; 赵莉辉; 曾忠玲

    2016-01-01

    目的:探讨白内障复明术中l期人工晶状体睫状沟缝线固定手术的技巧,并发症和发生原因及处理方法。  方法:回顾36例37眼l期行人工晶状体睫状沟缝线固定术手术方法,并对手术效果及并发症予以分析。  结果:术后随访3~24lo,最佳矫正视力≥0.8者4眼(11%),0.4~0.8者19眼(51%),0.1~0.3者14眼(38%)。主要并发症为角膜轻水肿内皮混浊10眼(27%),玻璃体出血3眼(8%),所有患者无严重并发症。  结论:l期人工晶状体睫状沟缝线固定术是白内障常规手术方法的补充,安全、有效,可显著提高视力,减少并发症的发生。%AlM:To investigate the surgical skills, the causes and the treatment of the complications of intraocular lens implantation sutured in ciliary sulcus at l phase in cataract surgery. METHODS:Thirty-six cases ( 37 eyes ) that couldn’t implant intraocular lens normally were treated with intraocular lens sutured in ciliary sulcus. The surgical effect and complications were analyzed. RESULTS:Patients were followed up for 3-24mo. The postoperative best corrected visual acuity( BCVA) was ≥0. 8 in 4 eyes(11%), 0. 4-0. 8 in 19 eyes (51%), 0. 1-0. 3 in 14 eyes ( 38%) . There were 10 eyes ( 27%) with corneal endothelium edema, 3 eyes ( 8%) with vitreous hemorrhage, and all the symptoms disappeared after dealing. There were no serious complications and sequelae in all the patients CONCLUSlON:lntraocular lens implantation sutured in ciliary sulcus at l phase in cataract surgery is a complement of the conventional surgery method for cataract with safety and effectiveness. lt can increase the vision significantly and reduce the complications.

  13. The management of YAG combined with the ciliary sulcus suture fixing for lens dislocation into the vitreous cavity%YAG激光联合缝线固定术处理人工晶状体坠入玻璃体腔

    Institute of Scientific and Technical Information of China (English)

    谭吉林; 苟甫伟; 龚力力; 唐建; 王心莲; 张鹏辉

    2010-01-01

    目的 探讨YAG激光联合缝线固定术治疗人工晶状体坠入玻璃体腔的术后效果.方法 充分散瞳后用YAG激光切开残存的囊膜,让患者俯卧位使人工晶状体脱入前房,然后用改良的人工晶状体缝线固定术将该人工晶状体固定于睫状沟.结果 12例中有11例的人工晶状体成功固定于睫状沟,这11例术后1周均达到和超过术前最佳矫正视力. 1例用该法没能使人工晶状体脱入前房,而建议行玻切手术.结论 用YAG联合后房型人工晶状体缝线固定术处理人工晶状体坠入玻璃体腔,是一种操作简单、创伤小、固定可靠、效果良好的手术方式,大部分患者不需再行玻切术,也不需将人工晶状体从眼球内取出.%Objective To explore a simple and reliable surgical treatment for intraocular lens (IOL) falling into the vitreous cavity and to evaluate its postoperative results. Methods Firstly, the pupil fully dilated and the remnants of capsular incised by YAG, then, to enable patients to prone position to make IOL off into the anterior chamber, and finally, the IOL fixed in the ciliary sulcus by using an improved suture fixation method. Results Eleven of the 12 cases had been successful with the lens fixed in the ciliary sulcus and the patients had regained as good as pre-operation best corrected visual acuity (BCVA) or better than it within a week, while 1 case was not successful to fix and was advised to receive vitrectomy. Conclusions YAG combined with the ciliary sulcus suture fixing is a simple, less traumatic, reliable, satisfying surgical method for those IOL dislocation into the vitreous cavity.

  14. Teaching Bodies in Place

    Science.gov (United States)

    Jones, Stephanie; Woglom, James F.

    2013-01-01

    Background/Context: This piece draws on literature in justice-oriented teacher education, feminist pedagogy, and postmodern notions of bodies and place to make sense of data generated from a three-year study of an undergraduate teacher education course. A feminist lens was used to engage a body- and place-focused pedagogy that aimed to engage…

  15. Body Basics Library

    Science.gov (United States)

    ... a Friend Who Cuts? About the Body Basics Library KidsHealth > For Teens > About the Body Basics Library Print A A A Text Size Did you ... system, part, and process works. Use this medical library to find out about basic human anatomy, how ...

  16. Healthy Mouth, Healthy Body

    Science.gov (United States)

    FOR THE DENTAL PATIENT ... Healthy mouth, healthy body T he mouth is a window into the health of the body. It can show signs of nutri- tional ... Sjögren’s syndrome—may first become apparent because of mouth lesions or other oral problems. The mouth is ...

  17. Our Bodies Are Changing

    Institute of Scientific and Technical Information of China (English)

    程国清

    2004-01-01

    Have you ever thought about your body changes?Up to about the age of eight or nine,girls and boys look quite alike.They have similar shaped bodies(形体相似)and their voices sound almost the same.As they grow, all their organs(器官)grow,too.

  18. The Body Bites Back!

    Science.gov (United States)

    Alsop, Steve

    2011-01-01

    How should we think about the body in science education? What ought it mean to be alive and live within epistemologies and pedagogies? What does it mean to be human in science education? In response to Auli Arvola Orlander and Per-Olof Wickram's article, this essay explores some of the possibilities and questions that the body evokes in science…

  19. Body-building

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    正In recent years, more and more people spare no pains to join in the body-building group. People begin to take part in various fitness clubs or fitness centers in their spare time. This shows body-building has become an indispensable part of many people’s life.

  20. Bodies and Voices

    DEFF Research Database (Denmark)

    A wide-ranging collection of essays centred on readings of the body in contemporary literary and socio-anthropological discourse, from slavery and rape to female genital mutilation, from clothing, ocular pornography, voice, deformation and transmutation to the imprisoned, dismembered, remembered......, abducted or ghostly body, in Africa, Australasia and the Pacific, Canada, the Caribbean, Great Britain and Eire...

  1. Monitoring the normal body

    DEFF Research Database (Denmark)

    Nissen, Nina Konstantin; Holm, Lotte; Baarts, Charlotte

    2015-01-01

    of practices for monitoring their bodies based on different kinds of calculations of weight and body size, observations of body shape, and measurements of bodily firmness. Biometric measurements are familiar to them as are health authorities' recommendations. Despite not belonging to an extreme BMI category......, they translate such measurements and recommendations in meaningful ways to fit their everyday life. Conclusions : Normal-weight and moderately overweight people are concerned with their body size and continuously monitor it. Future health promotion work should consider the kind of practices already established...... provides us with knowledge about how to prevent future overweight or obesity. This paper investigates body size ideals and monitoring practices among normal-weight and moderately overweight people. Methods : The study is based on in-depth interviews combined with observations. 24 participants were...

  2. The Semiotic Body

    DEFF Research Database (Denmark)

    Hoffmeyer, Jesper

    2008-01-01

    Most bodies in this world do not have brains and the minority of animal species that do have brained bodies are descendents from species with more distributed or decentralized nervous systems. Thus, bodies were here first, and only relatively late in evolution did the bodies of a few species grow...... of these seemingly ‘unnatural’ mental and even linguistic kinds of species. It is shown how the skin, on the one hand, makes us belong in the world, and on the other hand, is part of the huge landscape of membranes across which the semiotic self incessantly must be reconstituted. The discussion moves...... on to the intracellular world of signal transduction through which the activity of single cells are put to service for bodily needs. The paper further considers the mechanisms behind homeostasis and the semiotics of the psycho-neuro-endocrine integration in the body. The concept of semiotic emergence is introduced...

  3. 超声生物显微镜协助人工晶状体单襻睫状沟缝线固定术%Single haptic suture fixation of intraocular lens at ciliary sulcus aided by ultrasound biomicroscopy

    Institute of Scientific and Technical Information of China (English)

    姚长海; 王庆瑛

    2015-01-01

    Objective To evaluate the clinical efficacy of single haptic suture fixation of intraocular lens at ciliary sulcus aided with ultrasound biomicroscopy. Methods Eighteen eyes of eighteen patients were enrolled in this study. They had undergone single haptic suture fixation of intraocular lens at ciliary sulcus. Eleven eyes of eleven patients whose operation plans were designed under the pre-UBM examinations formed group A, while seven eyes of seven patients who received routine haptic suture fixation operation formed group B. After the operation, all the patients took UBM examination, and the data of position of needle insertion and intraocular lens were recorded. Results Position of stitch insertion in group A located in (0. 76 ± 0. 11)mm posterior to corneal limbus, while it was 1. 0 mm in group B. In group A, there were 6 cases(54. 55%) in which haptics of intraocular lens were precisely implanted at ciliary sulcus. While in group B, there was only one eye(14. 29%). In group A, the intraocular lens tilt was (0. 4635 ± 0. 1655) mm, and the intraocular lens decentration was (0.3543 ±0.3246)mm. In group B, they were (0.6913 ± 0. 2069) mm and (0. 8351 ± 0. 6286) mm respectively. Conclusion Single haptic suture fixation of intraocular lens at ciliary sulcus aided by ultrasound biomicroscopy, can reduce the occurance of postoperative intraocular lens tilt and decentration.%目的:评价超声生物显微镜( UBM)协助下人工晶状体单襻睫状沟缝线固定的手术方法和临床疗效。方法人工晶体单襻睫状沟缝线固定术18例(18眼)。其中,术前行UBM检查并依此设计手术方式者11例,设为A组;术前未行UBM检查,按常规手术方式植入者7例,设为B组。术后行UBM检查并记录术中进针位置及人工晶状体位置相关数据进行统计学比较。结果 A组的进针位置位于角膜缘后(0.76±0.11)mm;B组进针位置位于角膜缘后1.0 mm,A组中能确切植入睫状沟者6例(54.55%),而B组中仅1

  4. The body as art.

    Science.gov (United States)

    Barker, D J; Barker, M J

    2002-07-01

    For millennia people have altered the appearance of their bodies with cosmetics, jewellery, tattoos, piercings, and other surgical procedures. It would appear that they wish to conform to a perceived 'ideal body', although the actual appearance of such a body is subject to temporal, cultural and geographical change. In contemporary society the media are largely responsible for providing the yardsticks against which individual body shape is measured. Today the desired form is generally young, slim, tanned and blemish-free. Sadly, dissatisfaction with body image can be the source of great unhappiness and may even lead to suicide. Interested scholars have debated the meaning of beauty for centuries but it seems that every human society has its own standards. At the simplest it would appear that youth and symmetry are the most highly prized ingredients. There is no doubt that those who fit the conventional standards of attractiveness are treated better by society. Individuals have an inalienable right to their own body appearance, and to alter it as they see fit, however such modifications may not always be in their own best interests. Practitioners of cosmetic procedures must be alert to clients with histories of weight fluctuation, unrealistic body image, or low self-esteem. Psychological disorders may present with dysmorphophobic symptoms. Doctors providing cosmetic services need to be adept at diagnosing psychological illness. PMID:17147524

  5. Materiality, Practice and Body

    DEFF Research Database (Denmark)

    Johansen, Stine Liv; Skovbjerg-Karoff, Helle

    2009-01-01

    In order to understand the interaction between human and technology, the relationship must be emphasized as a triangulation between materiality, body and practice. By introducing play situations from a just finished empirical study in three bigger cities in Denmark, this paper will address...... the interplay from the human‟s point of view, as a body doing a certain practice, which is constantly produced by taking approaches which comes from phenomenology and practice theory. We introduce aspects of play understood as a dynamic between materiality, body and practice with the goal of inspiring not only...

  6. Body Dysmorphic Disorder

    Directory of Open Access Journals (Sweden)

    Perihan Cam Ray

    2012-12-01

    Full Text Available Body dysmorphic disorder is a type of mental illness, wherein the affected person is concerned with body image, manifested as excessive concern about and preoccupation with a perceived defect of their physical features. Although it is a common disease and has been defined in the literature over a century, it is not a well known disease. Chronic, treatment resistant and sometimes delusional nature could result in severe functional impairment. The diagnosis and appropriate therapy of disorder are crucial because of increased suicidality and reduction in life quality. In this article the symptoms, etiology, clinical features and treatment of body dysmorphic disorder are briefly reviewed.

  7. Adolescence and body image.

    Science.gov (United States)

    Weinshenker, Naomi

    2002-05-01

    Concerns about body image range from a normal desire to look attractive to a pathological concern with thinness or physical perfection. Today, more than ever, adolescents in America are prone to body image distortions and dissatisfaction. The reasons for this are multi-determined and include the influence of the media and cultural expectations, as well as a discrepancy between an adolescent's own physical characteristics and the expectations of his or her social environment. Adolescents with severe body image distortions are vulnerable to developing serious psychiatric disorders that can have life-threatening consequences. Schools can help by providing guidance and information in a time of uncertainty. PMID:12046161

  8. Cortical Lewy Body Dementia

    Directory of Open Access Journals (Sweden)

    W. R. G. Gibb

    1990-01-01

    Full Text Available In cortical Lewy body dementia the distribution of Lewy bodies in the nervous system follows that of Parkinson's disease, except for their greater profusion in the cerebral cortex. The cortical tangles and plaques of Alzheimer pathology are often present, the likely explanation being that Alzheimer pathology provokes dementia in many patients. Pure cortical Lewy body dementia without Alzheimer pathology is uncommon. The age of onset reflects that of Parkinson's disease, and clinical features, though not diagnostic, include aphasias, apraxias, agnosias, paranoid delusions and visual hallucinations. Parkinsonism may present before or after the dementia, and survival duration is approximately half that seen in Parkinson's disease without dementia.

  9. Nursing Cooperation of Closed Type Vitrectomy Via the Flat Part of Ciliary Body%经睫状体平坦部闭合式玻璃体切除术的护理配合

    Institute of Scientific and Technical Information of China (English)

    顾雪莲

    2001-01-01

    With the deve1opment of the ophthalmological surgery, PPV(Par Plana Vitrectony) has been widely used to treat retinal diseases. From June, 1999 to November, 2000, we have performed PPV in the total of 25 patients, and the results were satisfactory. This article introduced the experince of musing care during preoperation and operation in PPV, and the sterilization and the maintenance of special equipments.%随着眼科手术技术不断的发展,经睫状体平坦部闭合式玻璃体切除术越来越多地应用于眼底疾病的治疗。我院自1999年6月~2000年11月为25例严重眼底病变患者施行此项手术,疗效满意。本文介绍PPV手术的术前准备、术中配合、特殊器材的消毒与养护等方面的体会。

  10. Assessment of aqueous humor formation in human ciliary body in vitro:effect of ionic transport inhibitors%离体人眼睫状体房水分泌的测定以及药物的影响

    Institute of Scientific and Technical Information of China (English)

    吴仁毅; 杨芳; 尹金福; 姚克; Ivan Haefliger

    2009-01-01

    目的:建立离体的人眼睫状体房水分泌模型,研究离子转运抑制剂对房水分泌的影响.方法:应用改建的Ussing chamber,建立人眼睫状体房水分泌模型,检测人眼睫状体组织房水主动分泌以及跨睫状体短路电流(Isc).在此基础上,研究影响细胞离子转运的药物Na+-K+ATP酶抑制剂喹巴因(ouabain)、Na+-K+-2Cl-协同转运抑制剂丁苯氧酸(bumetanide)和碳酸酐酶抑制剂醋氮酰胺(acetazolamide)对房水分泌的影响.结果: 离体人眼睫状体Isc为(20.5±2.3) μA/cm2,房水的分泌量为(3.52±0.46) μL·h-1/cm2,并可维持4 h以上.在睫状体基质(血管)面给药喹巴因、丁苯氧酸或乙酰唑胺后,房水主动分泌分别下降87%(P<0.01),40%(P<0.01)和46%(P<0.01).结论:应用离体模型可有效测量人眼睫状体的主动房水分泌.影响细胞离子转运的药物能显著影响离体人眼睫状体的Isc和房水分泌,表明跨睫状体的离子转运参与了房水分泌过程.

  11. Markets, Bodies, Rhythms

    DEFF Research Database (Denmark)

    Borch, Christian; Bondo Hansen, Kristian; Lange, Ann-Christina

    2015-01-01

    This article explores the relationship between bodily rhythms and market rhythms in two distinctly different financial market configurations, namely the open-outcry pit (prevalent especially in the early 20th century) and present-day high-frequency trading. Drawing on Henri Lefebvre...... irrelevant. Yet high-frequency trading does change the role of the body rather than seeking to attune their bodies to the markets, high-frequency traders seek to calibrate their bodies to their algorithms. While the article demonstrates the usefulness of deploying Lefebvre's rhythmanalysis in analyses...... of financial markets, it also suggests that high-frequency trading in particular might produce new types of market rhythms that, contra Lefebvre, do not revolve around traders' bodies....

  12. Post Newtonian Rigid Body

    CERN Document Server

    Xu, C; Wu, X; Wu, Xuejun

    2004-01-01

    In this paper, it is the first time to construct a complete post-Newtonian (PN) model of a rigid body by means of a new constraint on the mass current density and mass density. In our PN rigid body model most of relations, such as spin vector proportional to the angular velocity, the definition on the moment of inertia tensor, the key relation between the mass quadrupole moment and the moment of inertia tensor, rigid rotating formulae of mass quadrupole moment and the moment of inertia tensor, are just the extension of the main relations in Newtonian rigid body model. When all of $1/c^2$ terms are neglected, the PN rigid body model and the corresponding formulae reduce to Newtonian version. The key relation is obtained in this paper for the first time, which might be very useful in the future application to problems in geodynamics and astronomy.

  13. Investigating body function

    International Nuclear Information System (INIS)

    This invention relates to the investigation of body function, especially small bowel function but also liver function, using bile acids and bile salts or their metabolic precursors labelled with radio isotopes and selenium or tellurium. (author)

  14. Unusual orbital foreign bodies

    Directory of Open Access Journals (Sweden)

    Agarwal P

    1993-01-01

    Full Text Available Retained intraorbital organic foreign bodies, particularly wooden, are commonly encountered in ophthalmologic practice. We treated two children who had sustained such injury while playing. They presented to us with non-healing sinus with purulent discharge. In one of the patients, X-rays and CT scan helped to clinch the diagnosis, whereas in the other patient diagnosis was possible by correlating history with clinical findings. Surgical exploration in both patients helped us to remove the foreign bodies. Surprisingly, both the foreign bodies were 7 cm long wooden pieces. We, however, caution that management of such cases should be conservative and that surgical exploration be done only in case of complication. From our experience, we recommend proper localisation by all possible means, blunt dissection, careful haemostasis coupled with excellent lighting and exposure in the atraumatic removal of intraorbital foreign bodies.

  15. [Towards the bionic body].

    Science.gov (United States)

    Andrieu, Bernard

    2011-06-01

    Global technological progress is enabling people to see their mutilated bodies assisted by sophisticated prostheses. Multi-disciplinary and associative care help patients relearn daily living activities and accomplish various exploits.

  16. Giant peritoneal loose bodies

    Directory of Open Access Journals (Sweden)

    Chris van Zyl

    2015-03-01

    Full Text Available Giant peritoneal loose bodies are rare lesions, originating from auto-amputated appendices epiploicae. They may cause urinary or gastrointestinal obstruction and, should the radiologist not be familiar with the entity, can potentially be confused with malignant or parasitic lesions.Familiarity with their characteristic computed tomographic features is essential to prevent unnecessary surgery in the asymptomatic patient. We present a case of a 70-year-old man diagnosed with two giant peritoneal loose bodies.

  17. Body Dysmorphic Disorder

    OpenAIRE

    Ray, Perihan Çam; Demirkol, Mehmet Emin; Tamam, Lut

    2012-01-01

    Body dysmorphic disorder is a type of mental illness, wherein the affected person is concerned with body image, manifested as excessive concern about and preoccupation with a perceived defect of their physical features. Although it is a common disease and has been defined in the literature over a century, it is not a well known disease. Chronic, treatment resistant and sometimes delusional nature could result in severe functional impairment. The diagnosis and appropriate therapy of disorder a...

  18. Body dysmorphic disorder

    OpenAIRE

    Veale, D

    2004-01-01

    Body dysmorphic disorder (BDD) is defined as a preoccupation with an "imagined" defect in one's appearance. Alternatively, where there is a slight physical anomaly, then the person's concern is markedly excessive. The preoccupation is associated with many time consuming rituals such as mirror gazing or constant comparing. BDD patients have a distorted body image, which may be associated with bullying or abuse during childhood or adolescence. Such patients have a poor quality of life, are soci...

  19. [Colorectal foreign bodies].

    Science.gov (United States)

    Thim, Troels; Laurberg, Søren

    2006-09-25

    A patient with a retained anally introduced colorectal foreign body or complications hereof needs appropriate treatment. The patient may be in danger and is certainly in discomfort. The problem is relatively rare; however, its incidence may be expected to increase. Guidelines for handling of the situation are lacking in many textbooks. Here, a suggestion for handling of a patient with a retained colorectal foreign body or complications hereof is presented. PMID:17032594

  20. Multichannel Human Body Communication

    Science.gov (United States)

    Przystup, Piotr; Bujnowski, Adam; Wtorek, Jerzy

    2016-01-01

    Human Body Communication is an attractive alternative for traditional wireless communication (Bluetooth, ZigBee) in case of Body Sensor Networks. Low power, high data rates and data security makes it ideal solution for medical applications. In this paper, signal attenuation for different frequencies, using FR4 electrodes, has been investigated. Performance of single and multichannel transmission with frequency modulation of analog signal has been tested. Experiment results show that HBC is a feasible solution for transmitting data between BSN nodes.

  1. Hacking the body

    OpenAIRE

    Baker, C

    2013-01-01

    This conference paper is available to download from the publisher’s website at the link below. Hacking the Body is a proposed collaborative re-search project that explores the use of the concept of 'hacking' to repurpose and re-imagine internal signals from the body through DIY biosensors and soft circuits. This paper outlines definitions of hacking and how these apply to workshops exploring how to create these sensors.

  2. Bursting bodies of water

    DEFF Research Database (Denmark)

    Rasmussen, Mattias Borg

    2014-01-01

    A silent threat is growing below receding glaciers: lakes are formed as the tongues of the glaciers draw back up the mountain, and huge and growing bodies of water beneath them are contained only be weak moraine walls.......A silent threat is growing below receding glaciers: lakes are formed as the tongues of the glaciers draw back up the mountain, and huge and growing bodies of water beneath them are contained only be weak moraine walls....

  3. Esophageal Foreign Bodies

    Directory of Open Access Journals (Sweden)

    Ufuk Cobanoglu

    2014-04-01

    Full Text Available Esophageal foreign body aspiration is a common event which can cause serious morbidity and mortality in the children and adult population. For that reason, early diagnosis and treatment are crucial for preventing these life threateining complications. Children most often ingest coins and toys whereas adults commonly tend to have problems with meat and bones. Esophageal foreign bodies are located at the cricopharyngeus muscle level in 70%, the thoracic esophagus in 15% and the gastroesophageal junction in the remaining 15%. Symptoms can vary according to the shape and structure of the ingested object, type of location, patient%u2019s age and complications caused by the foreign body. Delay in treatment, esophageal perforation and an underlying esophageal disease are poor prognostic factors. In treatment, observation, foley catheter, rigid or flexible esophagoscopy and removing the foreign body with a Magill forceps, pushing the foreign body into the stomach, giving intravenous glucagon and surgical treatment methods can be used. Rigid esophagoscopy is an effective and safe procedure for foreign body diagnosis and removal. Improved endoscopic experience and clinical management of thoracic surgeons led to reduced morbidity and mortality in recent years. Most of those emergencies of childhood are preventable. Family education is very important.

  4. The neural basis of body form and body action agnosia.

    Science.gov (United States)

    Moro, Valentina; Urgesi, Cosimo; Pernigo, Simone; Lanteri, Paola; Pazzaglia, Mariella; Aglioti, Salvatore Maria

    2008-10-23

    Visual analysis of faces and nonfacial body stimuli brings about neural activity in different cortical areas. Moreover, processing body form and body action relies on distinct neural substrates. Although brain lesion studies show specific face processing deficits, neuropsychological evidence for defective recognition of nonfacial body parts is lacking. By combining psychophysics studies with lesion-mapping techniques, we found that lesions of ventromedial, occipitotemporal areas induce face and body recognition deficits while lesions involving extrastriate body area seem causatively associated with impaired recognition of body but not of face and object stimuli. We also found that body form and body action recognition deficits can be double dissociated and are causatively associated with lesions to extrastriate body area and ventral premotor cortex, respectively. Our study reports two category-specific visual deficits, called body form and body action agnosia, and highlights their neural underpinnings.

  5. Retained, incarcerated oropharyngeal foreign bodies

    OpenAIRE

    Sonkhya, Nishi; Luckwani, Ashok; Mishra, Prakash; Yadav, Ramesh

    2006-01-01

    Foreign body ingestion is a well documented entity. Incarcerated oropharyngel foreign bodies are fre quently observed. Care should be taken for the symptoms like dysphagia and odynophagia, even if no positive history for foreign body ingestion is present. Two cases of incarcerated oropharyngeal foreign bodies are presented here who did not report with history of foreign body ingestion.

  6. Managing Regulatory Body Competence

    International Nuclear Information System (INIS)

    In 2001, the IAEA published TECDOC 1254, which examined the way in which the recognized functions of a regulatory body for nuclear facilities results in competence needs. Using the systematic approach to training (SAT), TECDOC 1254 provided a framework for regulatory bodies for managing training and developing and their maintaining their competence. It has been successfully used by many regulators. The IAEA has also introduced a methodology and an assessment tool - Guidelines for Systematic Assessment of Regulatory Competence Needs (SARCoN) - which provides practical guidance on analysing the training and development needs of a regulatory body and, through a gap analysis, guidance on establishing competence needs and how to meet them. In 2009, the IAEA established a steering committee (supported by a bureau) with the mission to advise the IAEA on how it could best assist Member States to develop suitable competence management systems for their regulatory bodies. The committee recommended the development of a safety report on managing staff competence as an integral part of a regulatory body's management system. This Safety Report was developed in response to this request. It supersedes TECDOC 1254, broadens its application to regulatory bodies for all facilities and activities, and builds upon the experience gained through the application of TECDOC 1254 and SARCoN and the feedback received from Member States. This Safety Report applies to the management of adequate competence as needs change, and as such is equally applicable to the needs of States 'embarking' on a nuclear power programme. It also deals with the special case of building up the competence of regulatory bodies as part of the overall process of establishing an 'embarking' State's regulatory system

  7. Clinical characteristics of primary ciliary dyskinesia in children%儿童原发性纤毛运动障碍的临床研究

    Institute of Scientific and Technical Information of China (English)

    徐保平; 申昆玲; 胡英惠; 冯雪莉; 李惠民; 郎志奇

    2008-01-01

    children.Methods Patients were all chosen from the inpatient department of Beijing Children's Hospital,Capital Medical University between 1990-2006.The tunics mucosa bronchiorum and/or nasal mucous membrane were gained through bronchoscope in children suspected to have PCD.The ciliary uhrnstructures were analyzed through the electron microscope.The clinical features and procedures of the diagnosis and differential diagnosis in children with PCD were analyzed.Results There were totally 26 children diagnosed as PCD with 10 (38.5%) Kartagener syndrome. All Kartagener syndrome children had mirror image dextrocardia with normal cardiac structure and situs inversus viscerurn- The broncheseopy performed in eight of 10 Kartagener syndrome children showed bronchus transposition. Twenty-six children came from twenty-five families.Although the siblings of four prohanda also had the symptoms of chronic cough with sputum,running nose and recurrent respiratory infections,only a boy and his sis~r were diagnosed as Kartagener syndrome simultaneously.Their parents and the other family members were healthy.Of the 26 patients,11 were boys and 15 were girls.The median age at diagnosis was 8.7 years.The age of onset was between the second day after dilivery and fifteen years old,median age was 3 years.The course of disease before diagnosis was eleven days to twelve years ( median 3.5 years).All the children had the symptom of cough,24 of which had productive cough.Seven cases were found to have clubbing fingers.Dynein arm defect was found in 10 children,6 of them had total absence of dynein arms and 4 had decreased dynein arm numbers.Microtube derangements were found in 8 children. One Kartngener syndrome child had a normal cilia structure.Bronchieetasis,consolidation and increased lung markings were found in 8,6 and 7 patients separately on the radiographic study.Twenty patients had sinusitis.Nine of sixteen children had decreased PEF,FEV1 and/or FEF25-75 on the pulmonary function test

  8. Body Building on Diamonds

    Institute of Scientific and Technical Information of China (English)

    Andrei P. Sommer; Dan Zhu; Tim Scharnweber; Hans-Joerg Fecht

    2009-01-01

    Whereas conservative therapies aim to stall the advance of disease, regenerative medicine strives to reverse it. The capacity of most tissues to regenerate derives from stem cells, but there are a number of barriers which have to be circumvented before it will be possible to use stem-cell-based therapies. Such therapies, however, are expected to improve human health enormously,and knowledge gained from studying stem cells in culture and in model organisms is now laying the groundwork for a new era of regenerative medicine. One of the most prominent methods to study stem cell differentiation is to let them to form embryoid bodies. Under favourable conditions any stem cell line will form embryoid bodies. However, the mechanism of the formation of embryoid bodies is not very well understood, and to produce them in the laboratory is in no way trivial - an important technical barrier in stem cell research. Recently, the embryoid body cultivation step has been successfully circumvented for the derivation of osteogenic cultures of embryonic stem cells. Here we report on a simple and reusable system to cultivate embryoid bodies in extremely short times. The method is inspired by the principles that lead to the establishment of the biomimetic triangle.

  9. Symptoms of Lewy Body Dementia

    Science.gov (United States)

    ... Lewy body dementia now! Donate Symptoms Lewy body dementia symptoms and diagnostic criteria Every person with LBD ... an umbrella term for two related clinical diagnoses, dementia with Lewy bodies and Parkinson's disease dementia. The ...

  10. Multiciliated cell basal bodies align in stereotypical patterns coordinated by the apical cytoskeleton.

    Science.gov (United States)

    Herawati, Elisa; Taniguchi, Daisuke; Kanoh, Hatsuho; Tateishi, Kazuhiro; Ishihara, Shuji; Tsukita, Sachiko

    2016-08-29

    Multiciliated cells (MCCs) promote fluid flow through coordinated ciliary beating, which requires properly organized basal bodies (BBs). Airway MCCs have large numbers of BBs, which are uniformly oriented and, as we show here, align linearly. The mechanism for BB alignment is unexplored. To study this mechanism, we developed a long-term and high-resolution live-imaging system and used it to observe green fluorescent protein-centrin2-labeled BBs in cultured mouse tracheal MCCs. During MCC differentiation, the BB array adopted four stereotypical patterns, from a clustering "floret" pattern to the linear "alignment." This alignment process was correlated with BB orientations, revealed by double immunostaining for BBs and their asymmetrically associated basal feet (BF). The BB alignment was disrupted by disturbing apical microtubules with nocodazole and by a BF-depleting Odf2 mutation. We constructed a theoretical model, which indicated that the apical cytoskeleton, acting like a viscoelastic fluid, provides a self-organizing mechanism in tracheal MCCs to align BBs linearly for mucociliary transport. PMID:27573463

  11. 'Bad boys'' Bodies

    DEFF Research Database (Denmark)

    Skårderud, Finn; Nygren, Pär; Edlund, Birgitta

    2005-01-01

    Children residing in care (hereafter referred to as childcare residents) are a risk¬group for emotional disturbances and behaviour problems. Based on existing knowledge of risk factors one would also expect this population to be a high-risk group for eating disorders and related body-image...... resident group; few differences between girls in the two samples; and a high frequency of having used anabolic?androgenic steroids among boys in care. Body-image problems among boys have hitherto been given little attention. The results call for increased efforts in describing and detecting patho...... disorders. The objective of this study was to describe pathological eating behaviour, dysfunctional body focusing and psychological symptoms in a sample of childcare residents compared with a non-clinical sample. Sixty-one childcare residents (aged 14?21 years, mean 16.2) and a non-clinical comparison group...

  12. Culture and body image

    Directory of Open Access Journals (Sweden)

    D. Alves

    2009-01-01

    Full Text Available The present study aims to investigate the relationship between culture and body image. We intend to know how socio-cultural factors influence the levels of satisfaction with body image. The emphasis is given to the cultural values as represented by the sociocultural norms of societies such as the United States of America and Europe. It is argued that through the media, the values of these industrialized societies are dissipated throughout the world provoking cultural changes and uniformization of behavioural standards. From the literature review, it is possible to conclude that body dissatisfaction is a reality to both sexes and a direct result of the non-conformity to cultural-esthetical patterns promoted by the profit-oriented societies.

  13. Body integrity identity disorder.

    Directory of Open Access Journals (Sweden)

    Rianne M Blom

    Full Text Available INTRODUCTION: Body Integrity Identity Disorder (BIID is a rare, infrequently studied and highly secretive condition in which there is a mismatch between the mental body image and the physical body. Subjects suffering from BIID have an intense desire to amputate a major limb or severe the spinal cord in order to become paralyzed. Aim of the study is to broaden the knowledge of BIID amongst medical professionals, by describing all who deal with BIID. METHODS: Somatic, psychiatric and BIID characteristic data were collected from 54 BIID individuals using a detailed questionnaire. Subsequently, data of different subtypes of BIID (i.e. wish for amputation or paralyzation were evaluated. Finally, disruption in work, social and family life due to BIID in subjects with and without amputation were compared. RESULTS: Based on the subjects' reports we found that BIID has an onset in early childhood. The main rationale given for their desire for body modification is to feel complete or to feel satisfied inside. Somatic and severe psychiatric co-morbidity is unusual, but depressive symptoms and mood disorders can be present, possibly secondary to the enormous distress BIID puts upon a person. Amputation and paralyzation variant do not differ in any clinical variable. Surgery is found helpful in all subjects who underwent amputation and those subjects score significantly lower on a disability scale than BIID subjects without body modification. CONCLUSIONS: The amputation variant and paralyzation variant of BIID are to be considered as one of the same condition. Amputation of the healthy body part appears to result in remission of BIID and an impressive improvement of quality of life. Knowledge of and respect for the desires of BIID individuals are the first steps in providing care and may decrease the huge burden they experience.

  14. 高度近视眼角膜水平径与睫状沟直径的相关性研究%Correlation between white-to-white diameter and ciliary sulcus diameter of high myopia eyes

    Institute of Scientific and Technical Information of China (English)

    高健; 廖荣丰

    2013-01-01

    差会随着前房加深逐渐增加.睫状沟垂直方向的距离大于水平方向.%Objective To investigate the ciliary sulcus diameters in horizontal and vertical axes and to analyze the correlation between white-to-white diameter and horizontal ciliary sulcus diameter for high myopia eyes at different anterior chamber depths.Methods IOL-Master was used to measure white-to-white diameters and anterior chamber depths of 108 high myopia eyes and verified by digital caliper.Based on the depth of central anterior chamber not including corneal thickness,the testing eyes were divided into three groups:shallow AC group(3.0 mm<ACD≤3.2 mm),medium AC group(3.2 mm<ACD≤3.4 mm),and deep AC group(ACD>3.4 mm).Furthermore,the horizontal and vertical ciliary sulcus diameters were checked by full-scale 50-Hz ultrasound biomicroscopy.The data of three AC groups were analyzed separately.The paired sample t-test was used to compare:white-to-white diameters and horizontal sulcus diameters,horizontal and vertical suclus diameters.The correlations between white-to-white diameters and horizontal sulcus diameters were assessed using linear regression analysis.The difference in the three groups of white-to-white diameters and ciliary sulcus diameters were compared by Student Newman Keuls test.Results The mean diameters and standard deviations of WTW diameters and horizontal ciliary sulcus diameters in the shallow AC group was,respectively,(11.46±0.38)and(11.57±0.32)mm(t=-2.849,P=0.007).Linear regression analysis found a statistically significant correlation between whiteto-white diameters and horizontal sulcus diameters with a coefficient of determinant of 0.797(P =0.000).The mean vertical suclus diameters was(12.27±0.48)mm.The difference between horizontal and vertical suclus diameters was statistically significant(t=-15.522,P=0.000).The mean diameters and standard deviations of WTW diameters and horizontal ciliary sulcus diameters in the medium AC group was

  15. Listening to the body?

    DEFF Research Database (Denmark)

    Ravn, Susanne; Christensen, Mette Krogh

    2013-01-01

    Based on a single case study of a Danish elite golfer, this article focuses on describing the different ways in which the golfer experiences the physicality of her body during training. The aim of the article is to explore how phenomenological insights concerning self-consciousness can be used...... suggest that the golfer’s experience of the physicality of her body can be considered in relation to three possible dimensions of self-consciousness: a pre-reflective subject-related dimension, a reflective object-directed dimension and a pre-reflective performative dimension. The pre...

  16. Post Newtonian Rigid Body

    OpenAIRE

    Xu, Chongming; Tao, Jin-he; Wu, Xuejun

    2003-01-01

    In this paper, it is the first time to construct a complete post-Newtonian (PN) model of a rigid body by means of a new constraint on the mass current density and mass density. In our PN rigid body model most of relations, such as spin vector proportional to the angular velocity, the definition on the moment of inertia tensor, the key relation between the mass quadrupole moment and the moment of inertia tensor, rigid rotating formulae of mass quadrupole moment and the moment of inertia tensor...

  17. Biometrics Bodies, Technologies, Biopolitics

    CERN Document Server

    Pugliese, Joseph

    2012-01-01

    Biometric technologies, such as finger- or facial-scan, are being deployed across a variety of social contexts in order to facilitate and guarantee identity verification and authentication. In the post-9/11 world, biometric technologies have experienced an extraordinary period of growth as concerns about security and screening have increased. This book analyses biometric systems in terms of the application of biopolitical power - corporate, military and governmental - on the human body. It deploys cultural theory in examining the manner in which biometric technologies constitute the body as a

  18. Very Young Children's Body Image: Bodies and Minds under Construction

    Science.gov (United States)

    Birbeck, David; Drummond, Murray

    2006-01-01

    In recent years research has recognised that notions of body image, body image ideals and body dissatisfaction develop much earlier than was once thought. Forty-seven children (25 male; 22 female) aged between 5 and 6 years were interviewed on three occasions over 12 months regarding their perceptions of body image. The interviews revealed…

  19. Motion of isolated bodies

    Energy Technology Data Exchange (ETDEWEB)

    Choquet-Bruhat, Yvonne [Academie des Sciences, 23 Quai de Conti, 75270 Paris Cedex 06 (France); Friedrich, Helmut [Max-Planck-Institut fuer Gravitationsphysik, Am Muehlenberg 1, 14476 Golm (Germany)

    2006-10-21

    It is shown that sufficiently smooth initial data for the Einstein-dust or the Einstein-Maxwell-dust equations with non-negative density of compact support develop into solutions representing isolated bodies in the sense that the matter field has spatially compact support and is embedded in an exterior vacuum solution.

  20. Motion of Isolated bodies

    CERN Document Server

    Choquet-Bruhat, Y; Choquet-Bruhat, Yvonne; Friedrich, Helmut

    2006-01-01

    It is shown that sufficiently smooth initial data for the Einstein-dust or the Einstein-Maxwell-dust equations with non-negative density of compact support develop into solutions representing isolated bodies in the sense that the matter field has spatially compact support and is embedded in an exterior vacuum solution.

  1. Understanding Body Language.

    Science.gov (United States)

    Sielski, Lester M.

    1979-01-01

    The article details the significance of nonverbal communication or body language for the counselor in interview situations. It gives background material of the research done in the area of nonverbal communication, and quotes extensively from the leaders in the field. (Author)

  2. Magnetic catalyst bodies

    NARCIS (Netherlands)

    Teunissen, Wendy; Bol, A.A.; Geus, John W.

    2001-01-01

    After a discussion about the importance of the size of the catalyst bodies with reactions in the liquid-phase with a suspended catalyst, the possibilities of magnetic separation are dealt with. Deficiencies of the usual ferromagnetic particles are the reactivity and the clustering of the particles.

  3. Brain, body and culture

    DEFF Research Database (Denmark)

    Geertz, Armin W.

    2010-01-01

    This essay sketches out a biocultural theory of religion which is based on an expanded view of cognition that is anchored in brain and body (embrained and embodied), deeply dependent on culture (enculturated) and extended and distributed beyond the borders of individual brains. Such an approach u...... to scholars of religion and be submitted to further hypotheses and tests by cognitive scientists....

  4. Identification of G Protein-Coupled Receptors (GPCRs in Primary Cilia and Their Possible Involvement in Body Weight Control.

    Directory of Open Access Journals (Sweden)

    Yoshihiro Omori

    Full Text Available Primary cilia are sensory organelles that harbor various receptors such as G protein-coupled receptors (GPCRs. We analyzed subcellular localization of 138 non-odorant GPCRs. We transfected GPCR expression vectors into NIH3T3 cells, induced ciliogenesis by serum starvation, and observed subcellular localization of GPCRs by immunofluorescent staining. We found that several GPCRs whose ligands are involved in feeding behavior, including prolactin-releasing hormone receptor (PRLHR, neuropeptide FF receptor 1 (NPFFR1, and neuromedin U receptor 1 (NMUR1, localized to the primary cilia. In addition, we found that a short form of dopamine receptor D2 (DRD2S is efficiently transported to the primary cilia, while a long form of dopamine receptor D2 (DRD2L is rarely transported to the primary cilia. Using an anti-Prlhr antibody, we found that Prlhr localized to the cilia on the surface of the third ventricle in the vicinity of the hypothalamic periventricular nucleus. We generated the Npy2r-Cre transgenic mouse line in which Cre-recombinase is expressed under the control of the promoter of Npy2r encoding a ciliary GPCR. By mating Npy2r-Cre mice with Ift80 flox mice, we generated Ift80 conditional knockout (CKO mice in which Npy2r-positive cilia were diminished in number. We found that Ift80 CKO mice exhibited a body weight increase. Our results suggest that Npy2r-positive cilia are important for body weight control.

  5. Identification of G Protein-Coupled Receptors (GPCRs) in Primary Cilia and Their Possible Involvement in Body Weight Control.

    Science.gov (United States)

    Omori, Yoshihiro; Chaya, Taro; Yoshida, Satoyo; Irie, Shoichi; Tsujii, Toshinori; Furukawa, Takahisa

    2015-01-01

    Primary cilia are sensory organelles that harbor various receptors such as G protein-coupled receptors (GPCRs). We analyzed subcellular localization of 138 non-odorant GPCRs. We transfected GPCR expression vectors into NIH3T3 cells, induced ciliogenesis by serum starvation, and observed subcellular localization of GPCRs by immunofluorescent staining. We found that several GPCRs whose ligands are involved in feeding behavior, including prolactin-releasing hormone receptor (PRLHR), neuropeptide FF receptor 1 (NPFFR1), and neuromedin U receptor 1 (NMUR1), localized to the primary cilia. In addition, we found that a short form of dopamine receptor D2 (DRD2S) is efficiently transported to the primary cilia, while a long form of dopamine receptor D2 (DRD2L) is rarely transported to the primary cilia. Using an anti-Prlhr antibody, we found that Prlhr localized to the cilia on the surface of the third ventricle in the vicinity of the hypothalamic periventricular nucleus. We generated the Npy2r-Cre transgenic mouse line in which Cre-recombinase is expressed under the control of the promoter of Npy2r encoding a ciliary GPCR. By mating Npy2r-Cre mice with Ift80 flox mice, we generated Ift80 conditional knockout (CKO) mice in which Npy2r-positive cilia were diminished in number. We found that Ift80 CKO mice exhibited a body weight increase. Our results suggest that Npy2r-positive cilia are important for body weight control. PMID:26053317

  6. 睫状肌训练法防治青少年近视的临床效果研究%Clinical effects of ciliaris training in prevention of juvenile myopia

    Institute of Scientific and Technical Information of China (English)

    欧召喜; 李建基; 张光辉

    2016-01-01

    目的:研究睫状肌训练防治青少年近视的临床效果。方法选择9~10周岁、裸眼视力为1.0~1.2的男女学生各50名,设为预防班;裸眼视力为0.6~0.8的男女学生各50名,设为治疗班。各班均按每组男女学生各25人随机分为1个训练组和1个对照组。训练组采用睫状肌训练法进行训练,对照组仅进行防治近视的科普宣教。实验时间为18个月。比较两组患者的视力、屈光度、眼轴长度等改善情况。结果实验前,各组裸眼视力、屈光度及眼轴长度比较,差异均无统计学意义(P >0.05)。实验后,各班训练组裸眼视力均高于实验前,对照组裸眼视力均低于实验前,训练组裸眼视力均高于对照组,差异有统计学意义(P <0.05);各班训练组屈光度均低于实验前,对照组屈光度均高于实验前,训练组屈光度均低于对照组,差异有统计学意义(P <0.05);各组实验前后眼轴长度比较,差异无统计学意义(P >0.05)。结论睫状肌训练对预防和治疗青少年近视均有明显效果。%Objective To study the clinical effects of ciliaris training in prevention of juvenile myopia.Methods 50 male and 50 female students aged from 9 to 1 0 years with uncorrected visual acuity ranging from 1 .0 to 1 .2 were selected as prevention class,and 50 male and 50 female students aged from 9 to 1 0 years with uncorrected visual acuity ranging from 0.6 to 0.8 were selected as therapy class.25 male and 25 female students in each class were randomized into training group or control group. Students in training groups were treated with ciliaris training while students in control groups were only treated with popularization and propaganda of myopia prevention.The experiment lasted for 1 8 months.The experimental period,students’eyesight,diop-ter and axial length were under compared.Results Before experiment,there was no statistically

  7. 人工晶状体睫状沟缝线固定联合瞳孔成形术%Ciliary sulcus suture fixation of intraocular lens combined with coreoplasty

    Institute of Scientific and Technical Information of China (English)

    肖博文; 曹维; 李俊岭

    2014-01-01

    目的 观察人工晶状体睫状沟缝线固定联合Siepser滑结缝线法瞳孔成形术处理复杂的眼前段外伤的临床效果.方法 回顾性分析复杂的眼前段外伤35例(35眼),行人工晶状体睫状沟缝线固定联合Siepser滑结法虹膜缝合及前部玻璃体切除术.随访3月,评估手术效果.结果 35眼术后视力均有不同程度提高,术后3月平均裸眼视力从术前的(0.04 ±0.02)提高到(0.39±0.18)(t=-11.4,P<0.01);术后3月平均瞳孔直径从术前的(6.23±2.01) mm缩小至(3.35±0.71) mm(=7.99,P<0.01);术后2眼眼压出现一过性升高,经降眼压治疗后恢复正常;所有术眼随访过程中均未出现虹膜前、后粘连或瞳孔膜闭等改变,术后35眼瞳孔均呈圆形或类圆形;无一例出现单眼复视症状;术后角膜内皮细胞数目均无明显下降;畏光和眩光等症状明显减轻或消失.结论 人工晶状体睫状沟缝线固定联合瞳孔成形术在复杂的眼前段外伤处理中是一种安全有效的手术方法.%Objective To observe the clinical efficacy of ciliary sulcus suture fixation of intraocular lens combined with Siepser slipknot suture coreoplasty for complex anterior segment trauma.Methods Thirty five eyes of 35 cases with complex anterior segment trauma,treated with ciliary sulcus suture fixation of intraocular lens combined with Siepser slipknot suture coreoplasty and anterior vitrectomy were analyzed.The follow-up time was 3 months.The surgical effects were analysed.Results Postoperatively,visual acuity was improved in all 35 eyes.After 3 months of operation,the average visual acuity was improved from preoperative (0.04 ±0.02) to postoperative (0.39 ± 0.18) (t =-11.4,P <0.01) and the average pupil diameter was reduced from preoperative (6.23 ± 2.01)mm to postoperative (3.35 ± 0.71) mm (t =7.99,P < 0.01).Two eyes had higher intraocular pressure which recovered soon after pressurelowering treatment.During the follow-up period,anterior or

  8. Body composition analysis: Cellular level modeling of body component ratios

    OpenAIRE

    Z. Wang; Heymsfield, S. B.; PI-SUNYER, F.X.; Gallagher, D.; PIERSON, R.N.

    2008-01-01

    During the past two decades, a major outgrowth of efforts by our research group at St. Luke’s-Roosevelt Hospital is the development of body composition models that include cellular level models, models based on body component ratios, total body potassium models, multi-component models, and resting energy expenditure-body composition models. This review summarizes these models with emphasis on component ratios that we believe are fundamental to understanding human body composition during growt...

  9. 全景超声生物显微镜对人眼睫状沟距离及前房直径的测量%Correlation between anterior chamber angle diameter and ciliary sulcus distance measured by Suowei panoramic 50-MHz digital ultrasound blomicroscopy

    Institute of Scientific and Technical Information of China (English)

    李德姣; 王宁利; 牟大鹏

    2011-01-01

    背景 睫状沟距离的直接测量对人工晶状体(I0L)植入术中IOL尺寸的选择具有重要意义.全景超声生物显微镜(UBM)的问世使在活体眼直接进行睫状沟距离的测量成为可能.目的 探讨人眼前房直径和睫状沟距离之间的相关性及其在不同前房深度人群中的差别.方法 将疑似青光眼患者30例30只右眼作为浅前房组,而具有高度近视(-7 ~-18 D)的30例30只右眼作为深前房组.应用全景超声生物显微镜(UBM)进行图像采集,每眼获取焦点位于虹膜平面的眼球水平方向3:00 ~9:00处的全景截面图像共3张.由同一测量者对每一幅图像的前房直径和睫状沟距离进行测量.取3幅图像测量值的均值为最终测量结果,用独立样本t检验和线性回归方程分析评估前房直径与睫状沟距离测量值的关系.结果 浅前房组的30眼中,全景UBM测得的前房直径为(11.49±0.75)mm,睫状沟距离为(10.97±0.86)mm,二者间的平均差值为(-5.14±0.55)mm,差异有统计学意义(t=-5.092,P=0.000),线性回归分析显示二者之间呈线性相关(R2=0.593,P=0.000),Pearson相关系数为0.767.深前房组的30眼中,全景UBM测得的前房直径均值为(12.69±0.67)mm;睫状沟距离为(12.31±0.61)mm,二者之间平均差值为(-0.38±0.17)mm,差异有统计学意义(t=-5.531,P=0.000);线性回归分析显示二者之间具有显著相关性(R2=0.699,P=0.000),Pearson相关系数为0.836.结论 全景UBM测得的睫状沟距离和前房直径呈明显正相关,深前房眼的相关性大于浅前房眼,该结果对于IOL植入术中IOL尺寸的选择具有重要意义.%Background Measurement of ciliary sulcus distance is important for phakic intraocular lens implantation.Suowei panoramic 50-MHz digital ultrasound biomicroscopy makes it possible for the direct measurement of ciliary sulcus distance.Objective The aim of this study was to investigate the correlation between anterior chamber(AC)angle diameter and

  10. ‘ SILENT’ LARYNGEAL FOREIGN BODY

    Directory of Open Access Journals (Sweden)

    Chandrasekhar

    2015-06-01

    Full Text Available Laryngeal foreign bodies in adults are rare. The foreign bodies accidentally entering the larynx are symptomatic in the form of choking , stridor or even death. We are presenting a rare case of foreign body in the larynx in a 42 year old male who was symptom free except for dysphonia. The foreign body was removed successfully under local anesthesia.

  11. Body Art: A Panel Discussion.

    Science.gov (United States)

    Surgenor, Peter; And Others

    1997-01-01

    Three camp directors discuss their policies regarding body art and body piercing. Only one director reported a strict policy prohibiting tattoos or body art based on standards that the camp portrays to families. However, all directors enforced policies prohibiting clothing or body art that mentions alcohol, tobacco, drug use, or inappropriate…

  12. Inclusion bodies in Plesiomonas shigelloides.

    OpenAIRE

    Pastian, M R; Bromel, M C

    1984-01-01

    Inclusion bodies were discovered in seven environmental isolates of Plesiomonas shigelloides and the P. shigelloides control (ATCC 14029). Differential staining indicated that the inclusion bodies may be composed of polyphosphates, and developmental stages of the bodies may occur. The inclusion bodies may be useful for rapid presumptive identification of this organism.

  13. Quasi Lp-Intersection Bodies

    Institute of Scientific and Technical Information of China (English)

    Wu Yang YU; Dong Hua WU; Gang Song LENG

    2007-01-01

    The purpose of this paper is to generalize the notion of intersection bodies to that of quasi Lp-intersection bodies. The Lp-analogs of the Busemann intersection inequality and the Brunn Minkowski inequality for the quasi Lp-intersection bodies are obtained. The Aleksandrov-Fenchel inequality for the mixed quasi Lp-intersection bodies is also established.

  14. Body image inflexibility mediates the relationship between body image evaluation and maladaptive body image coping strategies.

    Science.gov (United States)

    Mancuso, Serafino G

    2016-03-01

    Body image inflexibility, the unwillingness to experience negative appearance-related thoughts and emotions, is associated with negative body image and eating disorder symptoms. The present study investigated whether body image inflexibility mediated the relationship between body image evaluation and maladaptive body image coping strategies (appearance-fixing and experiential avoidance) in a college and community sample comprising 156 females aged 18-51 years (M=22.76, SD=6.96). Controlling for recruitment source (college vs. community), body image inflexibility fully mediated the relationship between body image evaluation and maladaptive body image coping strategies. Results indicated that an unwillingness to experience negative appearance-related thoughts and emotions is likely responsible for negative body image evaluation's relationship to appearance-fixing behaviours and experiential avoidance. Findings support extant evidence that interventions that explicitly target body image inflexibility, such as Acceptance and Commitment Therapy, may have utility in treating body dissatisfaction in nonclinical populations. PMID:26595857

  15. Body image inflexibility mediates the relationship between body image evaluation and maladaptive body image coping strategies.

    Science.gov (United States)

    Mancuso, Serafino G

    2016-03-01

    Body image inflexibility, the unwillingness to experience negative appearance-related thoughts and emotions, is associated with negative body image and eating disorder symptoms. The present study investigated whether body image inflexibility mediated the relationship between body image evaluation and maladaptive body image coping strategies (appearance-fixing and experiential avoidance) in a college and community sample comprising 156 females aged 18-51 years (M=22.76, SD=6.96). Controlling for recruitment source (college vs. community), body image inflexibility fully mediated the relationship between body image evaluation and maladaptive body image coping strategies. Results indicated that an unwillingness to experience negative appearance-related thoughts and emotions is likely responsible for negative body image evaluation's relationship to appearance-fixing behaviours and experiential avoidance. Findings support extant evidence that interventions that explicitly target body image inflexibility, such as Acceptance and Commitment Therapy, may have utility in treating body dissatisfaction in nonclinical populations.

  16. Preparation and Evaluation of Liposome-encapsulated Recombinant Human Ciliary Neurotrophic Factor%重组人睫状神经营养因子脂质体制备及性质

    Institute of Scientific and Technical Information of China (English)

    高剑坤; 范开; 侯再金; 张兵兵; 杨黎; 胡春兰

    2011-01-01

    OBJECTIVE To enhance the penetration ability to cross the blood-brain barrier efficiently of recombinant human ciliary reurotrophic factor (rhCNTF) by encapsulated into liposome. METHODS rhCNTF liposomes were prepared by thin film dispersion-sonication method. Encapsulation efficiency was detected by HPLC. Mice were killed 0, 2, 6, 12 and 24 h after i.v. administration of rhCNTF solution, rhCNTF liposomes or saline, and then the content of rhCNTF in brain tissues was analyzed by ELISA. RESULTS rhCNTF liposomes were regular in its morphology with a mean diameter of 85.0 nm. The entrapment efficiency of rhCNTF was 93.14%. rhCNTF in mice brain tissue were different between rhCNTF solution group and rhCNTF liposomes group. CONCLUSION Liposome-encapsulated rhCNTF could efficiently cross the blood brain barrier,which provides a basis for further application and research of rhCNTF.%目的 制备重组人睫状神经营养因子(recombinant human ciliary neurotrophic factor,rhCNTF),脂质体,提高rhCNTF穿透血脑屏障能力.方法 采用薄膜超声法制备rhCNTF脂质体,测定包封率.分别用rhCNTF原液、rhCNTF脂质体及生理盐水尾静脉注射Balb/C小鼠,0,2,6,12,24 h后测小鼠脑组织中rhCNTF含量.结果 制得的rhCNTF脂质体形态圆整,平均直径为85.0 nm,包封率为93.14%.用ELISA法测定小鼠脑组织中rhCNTF的含量,rhCNTF原液组和rhCNTF脂质体组存在显著差异.结论 制备的rbCNTF脂质体能提高rhCNTF穿越血脑屏障的能力,为rhCNTF的进一步应用和研究提供了依据.

  17. Body composition in detoxified alcoholics.

    Science.gov (United States)

    York, J L; Pendergast, D E

    1990-04-01

    Body composition was evaluated in healthy detoxified alcoholics (aged 20-39) and lifestyle controls, with the expectation that prolonged, excessive consumption of alcohol may bring about nutritional or toxicologic alterations in the relationship between body fat and lean body mass. Body fat was assessed by measurements of skin-fold thickness and by means of bioelectric impedance methodology. No noteworthy differences were observed between alcoholics and controls with regard to the relationship between lean body mass and body fat or in the relationship between extracellular and intracellular water. It would appear that 15-20 years of heavy alcohol consumption does not necessarily alter body composition in healthy, young alcoholics. PMID:2190482

  18. Stirring by swimming bodies

    International Nuclear Information System (INIS)

    We consider the stirring of an inviscid fluid caused by the locomotion of bodies through it. The swimmers are approximated by non-interacting cylinders or spheres moving steadily along straight lines. We find the displacement of fluid particles caused by the nearby passage of a swimmer as a function of an impact parameter. We use this to compute the effective diffusion coefficient from the random walk of a fluid particle under the influence of a distribution of swimming bodies. We compare with the results of simulations. For typical sizes, densities and swimming velocities of schools of krill, the effective diffusivity in this model is five times the thermal diffusivity. However, we estimate that viscosity increases this value by two orders of magnitude.

  19. Imaging body armor.

    Science.gov (United States)

    Harcke, H Theodore; Schauer, David A; Harris, Robert M; Campman, Steven C; Lonergan, Gael J

    2002-04-01

    This study examined the feasibility of performing radiographic studies on patients wearing standard-issue body armor. The Kevlar helmet, fragmentation vest, demining suit sleeve, and armor plate were studied with plain film and computed tomography in a simulated casualty situation. We found that the military helmet contains metal screws and metal clips in the headband, but diagnostic computed tomographic images can be obtained. Kevlar, the principal component of soft armor, has favorable photon attenuation characteristics. Plate armor of composite material also did not limit radiographic studies. Therefore, when medically advantageous, patients can be examined radiographically while wearing standard military body armor. Civilian emergency rooms should be aware of these observations because law enforcement officers wear similar protective armor. PMID:11977874

  20. Marijuana and Body Weight

    OpenAIRE

    Sansone, Randy A.; Sansone, Lori A.

    2014-01-01

    Acute marijuana use is classically associated with snacking behavior (colloquially referred to as “the munchies”). In support of these acute appetite-enhancing effects, several authorities report that marijuana may increase body mass index in patients suffering from human immunodeficiency virus and cancer. However, for these medical conditions, while appetite may be stimulated, some studies indicate that weight gain is not always clinically meaningful. In addition, in a study of cancer patien...

  1. Body Dysmorphic Disorder

    OpenAIRE

    Perihan Cam Ray; Mehmet Emin Demirkol; Lut Tamam

    2012-01-01

    Body dysmorphic disorder (BDD) is a relatively common disorder that consists of a distressing or impairing preoccupation with imagined or slight defects in appearance. BDD is commonly considered to be an obsessivecompulsive spectrum disorder, based on similarities it has with obsessive-compulsive disorder. It is important to recognize and appropriately treat BDD, as this disorder is associated with marked impairment in psychosocial functioning, notably poor quality of life, and high suicidali...

  2. PML body meets telomere

    OpenAIRE

    Chung, Inn; Osterwald, Sarah; Deeg, Katharina I.; Rippe, Karsten

    2012-01-01

    The unlimited proliferation potential of cancer cells requires the maintenance of their telomeres. This is frequently accomplished by reactivation of telomerase. However, in a significant fraction of tumors an alternative lengthening of telomeres (ALT) mechanism is active. The molecular mechanism of the ALT pathway remains elusive. In particular, the role of characteristic complexes of promyelocytic leukemia nuclear bodies (PML-NBs) with telomeres, the ALT-associated PML-NBs (APBs), is curren...

  3. Stab resistant body armour

    OpenAIRE

    Horsfall, Ian

    2000-01-01

    There is now a widely accepted need for stab resistant body armour for the police in the UK. However, very little research has been done on knife resistant systems and the penetration mechanics of sharp projectiles are poorly understood. This thesis explores the general background to knife attack and defence with a particular emphasis on the penetration mechanics of edged weapons. The energy and velocity that can be achieved in stabbing actions has been determined for a numb...

  4. Splintered body image

    OpenAIRE

    Castillo Ballén, Sonia

    2014-01-01

    Body image presentation, which made painting morevisual in the 20th century, offers visions of splinteredbodies in different versions of its extention in space andin its proyection in time. This splintered vision in visualpainting was structured by diverse factors, such as theearlier development of photography, and later by audiovisual,videography and rising virtual media. Painting´sclose-up shots on corporal action in the middle of the20th century, allowed us to question new paradigms ofcont...

  5. Few-body physics

    International Nuclear Information System (INIS)

    Few-body hadronic observables play an essential role in a wide number of processes relevant for both particle and nuclear physics. In order for Lattice QCD to offer insight into the interpretation of few-body states, a theoretical infrastructure must be developed to map Euclidean-time correlation functions to the desired Minkowski-time few-body observables. In this talk, I will first review the formal challenges associated with the studies of such systems via Lattice QCD, as first introduced by Maiani and Testa, and then review methodology to circumvent said limitations. The first main example of the latter is the formalism of Luscher to analyze elastic scattering and a second is the method of Lellouch & Luscher to analyze weak decays. I will then proceed to discus recent theoretical generalizations of these frameworks that allow for the determination of scattering amplitudes, resonances, transition and elastic form factors. Finally, I will outline outstanding problems, including those that are now beginning to be addressed.

  6. Few-body physics

    Energy Technology Data Exchange (ETDEWEB)

    Briceno, Raul [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States)

    2015-05-01

    Few-body hadronic observables play an essential role in a wide number of processes relevant for both particle and nuclear physics. In order for Lattice QCD to offer insight into the interpretation of few-body states, a theoretical infrastructure must be developed to map Euclidean-time correlation functions to the desired Minkowski-time few-body observables. In this talk, I will first review the formal challenges associated with the studies of such systems via Lattice QCD, as first introduced by Maiani and Testa, and then review methodology to circumvent said limitations. The first main example of the latter is the formalism of Luscher to analyze elastic scattering and a second is the method of Lellouch & Luscher to analyze weak decays. I will then proceed to discus recent theoretical generalizations of these frameworks that allow for the determination of scattering amplitudes, resonances, transition and elastic form factors. Finally, I will outline outstanding problems, including those that are now beginning to be addressed.

  7. Body Language in Drama

    Directory of Open Access Journals (Sweden)

    Nihat ÇALIŞKAN

    2005-12-01

    Full Text Available Drama is an act continuing life-long of a human and is an art of living. Owing to drama a child can gain the apportunity of practising and learning his life in games that he likes most. Today drama is scrutinized in four subtitles as creative, educational, psychodrama and sociodrama. The concepts about drama can be explained as creaticeness, metaksis, interraction, action, activity and empathy.Drama is the explanation of a sense or thought by motion, mimic, gesture and in words. In other words it is the animation of a situation or a subject using body language, reflecting by living, transforming into life.By using the body language consciously and effectively, it has an important function at dramatizing the events, getting students’ attention in education, concretizing abstract expressions, at stres accent and increasing the understandability of messages.Pantomime technic in drama method has a great importance at using the activities and human’s world consciously and animating the expressions and events. Because a teacher’s acting biology is importatnt an educational period.In this study related to drama expression, the importance of creative, educational, psychodrama, sociodrama, body language and pandomime technic in educational period has been tried to explaired.

  8. Implicit Beliefs about Ideal Body Image Predict Body Image Dissatisfaction

    Directory of Open Access Journals (Sweden)

    Niclas eHeider

    2015-10-01

    Full Text Available We examined whether implicit measures of actual and ideal body image can be used to predict body dissatisfaction in young female adults. Participants completed two Implicit Relational Assessment Procedures (IRAPs to examine their implicit beliefs concerning actual (e.g., I am thin and desired ideal body image (e.g., I want to be thin. Body dissatisfaction was examined via self-report questionnaires and rating scales. As expected, differences in body dissatisfaction exerted a differential influence on the two IRAP scores. Specifically, the implicit belief that one is thin was lower in participants who exhibited a high degree of body dissatisfaction than in participants who exhibited a low degree of body dissatisfaction. In contrast, the implicit desire to be thin (i.e., thin ideal body image was stronger in participants who exhibited a high level of body dissatisfaction than in participants who were less dissatisfied with their body. Adding further weight to the idea that both IRAP measures captured different underlying constructs, we also observed that they correlated differently with body mass index, explicit body dissatisfaction, and explicit measures of actual and ideal body image. More generally, these findings underscore the advantage of using implicit measures that incorporate relational information relative to implicit measures that allow for an assessment of associative relations only.

  9. Rotor/body aerodynamic interactions

    Science.gov (United States)

    Betzina, M. D.; Smith, C. A.; Shinoda, P.

    1985-01-01

    A wind tunnel investigation was conducted in which independent, steady state aerodynamic forces and moments were measured on a 2.24 m diam. two bladed helicopter rotor and on several different bodies. The mutual interaction effects for variations in velocity, thrust, tip-path-plane angle of attack, body angle of attack, rotor/body position, and body geometry were determined. The results show that the body longitudinal aerodynamic characteristics are significantly affected by the presence of a rotor and hub, and that the hub interference may be a major part of such interaction. The effects of the body on the rotor performance are presented.

  10. Tess:a Phenomenal Body

    Institute of Scientific and Technical Information of China (English)

    朱利华

    2012-01-01

      Few people read Tess from Maurice Merleau-Ponty’s“body theory”. In Phenomenology of Perception, Merleau-Ponty states that it is body as the subject that perceives the world; such body is a phenomenal body in contrast to an objective body. Tess’s“being in the world”through the body connotates a new way of looking at the classic Tess. Tess’s way of perceiv⁃ing and understanding the world also enlightens us about relationship between human and self, human and other, human and soci⁃ety, human and nature.

  11. Implicit body representations and the conscious body image

    OpenAIRE

    Longo, M. R.; Haggard, P.

    2012-01-01

    Recent studies have revealed that somatosensory processing relies on a class of implicit body representations showing large distortions of size and shape. The relation between these representations and the conscious body image remains unclear. Dissociations have been reported in the clinical literature on eating disorders between different body image measures, with larger and more consistent distortions found with depictive measures, in which participants compare their body to a visual depict...

  12. Body searching for freedom

    OpenAIRE

    Silvia Bortolás

    2008-01-01

    The aim of this study is to analyze how the previous council of class of the third bimester/ 2000 is accomplished, as well as to observe students’s resistance to the rules of Escola Estadual de 1º Grau Tancredo Neves – Santa Maria – RS. Although the school follows a pegagogical liberating proposal based on Paulo Freire’s view, the council of class still call their students well behaved and badly behaved during their disciplinary practices. In that scenary the body is viewed and treated throug...

  13. The Mallory body

    DEFF Research Database (Denmark)

    Jensen, K; Gluud, C

    1994-01-01

    Drawing on data from a previously published literature survey on the clinical and experimental epidemiology of the Mallory body, we discuss current theories on its development in a pro et contra manner. Conclusions have been largely left open to the interpretations of the reader because many...... be found with disease severity or duration in alcoholic liver diseases. The roles of vitamin A deficiency and protein-calorie malnutrition are circumstantial. Drugs known to have calcium-antagonist properties and the physiological characteristics of the stress-response protein ubiquitin support the concept...

  14. Truth, body and religion

    Directory of Open Access Journals (Sweden)

    Jarl-Thure Eriksson

    2011-01-01

    Full Text Available This paper is based on the words of welcome to the symposium on Religion and the Body on 16 June 2010. In a religious context ‘truth’ is like a mantra, a certain imperative to believe in sacred things. The concept of truth and falseness arises, when we as humans compare reality, as we experience it through our senses, with the representation we have in our memory, a comparison of new information with stored information. If we look for the truth, we have to search in the human mind. There we will also find religion.

  15. Body shape preferences: associations with rater body shape and sociosexuality.

    Science.gov (United States)

    Price, Michael E; Pound, Nicholas; Dunn, James; Hopkins, Sian; Kang, Jinsheng

    2013-01-01

    There is accumulating evidence of condition-dependent mate choice in many species, that is, individual preferences varying in strength according to the condition of the chooser. In humans, for example, people with more attractive faces/bodies, and who are higher in sociosexuality, exhibit stronger preferences for attractive traits in opposite-sex faces/bodies. However, previous studies have tended to use only relatively simple, isolated measures of rater attractiveness. Here we use 3D body scanning technology to examine associations between strength of rater preferences for attractive traits in opposite-sex bodies, and raters' body shape, self-perceived attractiveness, and sociosexuality. For 118 raters and 80 stimuli models, we used a 3D scanner to extract body measurements associated with attractiveness (male waist-chest ratio [WCR], female waist-hip ratio [WHR], and volume-height index [VHI] in both sexes) and also measured rater self-perceived attractiveness and sociosexuality. As expected, WHR and VHI were important predictors of female body attractiveness, while WCR and VHI were important predictors of male body attractiveness. Results indicated that male rater sociosexuality scores were positively associated with strength of preference for attractive (low) VHI and attractive (low) WHR in female bodies. Moreover, male rater self-perceived attractiveness was positively associated with strength of preference for low VHI in female bodies. The only evidence of condition-dependent preferences in females was a positive association between attractive VHI in female raters and preferences for attractive (low) WCR in male bodies. No other significant associations were observed in either sex between aspects of rater body shape and strength of preferences for attractive opposite-sex body traits. These results suggest that among male raters, rater self-perceived attractiveness and sociosexuality are important predictors of preference strength for attractive opposite

  16. Body shape preferences: associations with rater body shape and sociosexuality.

    Directory of Open Access Journals (Sweden)

    Michael E Price

    Full Text Available There is accumulating evidence of condition-dependent mate choice in many species, that is, individual preferences varying in strength according to the condition of the chooser. In humans, for example, people with more attractive faces/bodies, and who are higher in sociosexuality, exhibit stronger preferences for attractive traits in opposite-sex faces/bodies. However, previous studies have tended to use only relatively simple, isolated measures of rater attractiveness. Here we use 3D body scanning technology to examine associations between strength of rater preferences for attractive traits in opposite-sex bodies, and raters' body shape, self-perceived attractiveness, and sociosexuality. For 118 raters and 80 stimuli models, we used a 3D scanner to extract body measurements associated with attractiveness (male waist-chest ratio [WCR], female waist-hip ratio [WHR], and volume-height index [VHI] in both sexes and also measured rater self-perceived attractiveness and sociosexuality. As expected, WHR and VHI were important predictors of female body attractiveness, while WCR and VHI were important predictors of male body attractiveness. Results indicated that male rater sociosexuality scores were positively associated with strength of preference for attractive (low VHI and attractive (low WHR in female bodies. Moreover, male rater self-perceived attractiveness was positively associated with strength of preference for low VHI in female bodies. The only evidence of condition-dependent preferences in females was a positive association between attractive VHI in female raters and preferences for attractive (low WCR in male bodies. No other significant associations were observed in either sex between aspects of rater body shape and strength of preferences for attractive opposite-sex body traits. These results suggest that among male raters, rater self-perceived attractiveness and sociosexuality are important predictors of preference strength for

  17. Help! Is This My Body?

    Science.gov (United States)

    ... your dog, or throw a Frisbee with your friends. About three quarters of all teens quit sports around the time their bodies develop. Often it's because the changes in their bodies influence which sports they compete in. Although you can ...

  18. Introduction to the Human Body

    Science.gov (United States)

    ... Resources Archived Modules Updates Introduction to the Human Body Human beings are arguably the most complex organisms on ... for the benefit of the total being. The human body is a single structure but it is made ...

  19. R-body-producing bacteria.

    Science.gov (United States)

    Pond, F R; Gibson, I; Lalucat, J; Quackenbush, R L

    1989-03-01

    Until 10 years ago, R bodies were known only as diagnostic features by which endosymbionts of paramecia were identified as kappa particles. They were thought to be limited to the cytoplasm of two species in the Paramecium aurelia species complex. Now, R bodies have been found in free-living bacteria and other Paramecium species. The organisms now known to form R bodies include the cytoplasmic kappa endosymbionts of P. biaurelia and P. tetraurelia, the macronuclear kappa endosymbionts of P. caudatum, Pseudomonas avenae (a free-living plant pathogen), Pseudomonas taeniospiralis (a hydrogen-oxidizing soil microorganism), Rhodospirillum centenum (a photosynthetic bacterium), and a soil bacterium, EPS-5028, which is probably a pseudomonad. R bodies themselves fall into five distinct groups, distinguished by size, the morphology of the R-body ribbons, and the unrolling behavior of wound R bodies. In recent years, the inherent difficulties in studying the organization and assembly of R bodies by the obligate endosymbiont kappa, have been alleviated by cloning and expressing genetic determinants for these R bodies (type 51) in Escherichia coli. Type 51 R-body synthesis requires three low-molecular-mass polypeptides. One of these is modified posttranslationally, giving rise to 12 polypeptide species, which are the major structural subunits of the R body. R bodies are encoded in kappa species by extrachromosomal elements. Type 51 R bodies, produced in Caedibacter taeniospiralis, are encoded by a plasmid, whereas bacteriophage genomes probably control R-body synthesis in other kappa species. However, there is no evidence that either bacteriophages or plasmids are present in P. avenae or P. taeniospiralis. No sequence homology was detected between type 51 R-body-encoding DNA and DNA from any R-body-producing species, except C. varicaedens 1038. The evolutionary relatedness of different types of R bodies remains unknown. PMID:2651865

  20. Lp-mixed intersection bodies

    Institute of Scientific and Technical Information of China (English)

    ZHAO ChangJian

    2008-01-01

    In this paper the author first introduce a new concept of Lp-dual mixed volumes of star bodies which extends the classical dual mixed volumes. Moreover, we extend the notions of Lp-intersection body to Lp-mixed intersection body. Inequalities for Lp-dual mixed volumes of Lp-mixed intersection bodies are established and the results established here provide new estimates for these type of inequalities.

  1. The role of radiotherapy in the treatment of meningeal melanocytoma; Die Rolle der Strahlentherapie bei der Behandlung des Meningealen Melanozytoms

    Energy Technology Data Exchange (ETDEWEB)

    Rades, D.; Fehlauer, F.; Alberti, W. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Universitaetsklinikum Hamburg-Eppendorf (Germany); Schild, S. [Mayo Clinic, Scottsdale, Arizona (United States). Dept. of Radiation Oncology; Tatagiba, M. [Klinik fuer Neurochirurgie, Eberhard-Karls-Universitaet Tuebingen (Germany); Molina, H. [Clinica y Maternidad Suizo-Argentina, Buenos Aires (Argentina). Lab. of Neuropathology

    2004-07-01

    Because of its great rarity an optimal therapeutic regime for this entity has yet to be found. This holds especially for the role of radiotherapy following incomplete resection. The present analysis compares four therapeutic regimes in terms of local control and survival, thus contributing to the formulation of an optimal treatment concept. [German] Angesichts der grossen Seltenheit dieser Entitaet ist das optimale Therapieregime bislang nicht hinreichend geklaert. Dies gilt insbesondere fuer die Rolle der Strahlentherapie nach inkompletter Resektion. Die vorliegende Analyse vergleicht vier Therapieschemata im Hinblick auf die lokale Kontrolle und das Gesamtueberleben, um einen Beitrag zur Definition eines optimalen Behandlungskonzepts zu leisten. (orig.)

  2. Bodies, embodiment and ubiquitous computing

    DEFF Research Database (Denmark)

    Schick, Lea; Malmborg, Lone

    2010-01-01

    This paper advocates the future of the body as a distributed and shared embodiment; an unfolded body that doesn't end at one's skin, but emerges as intercorporeality between bodies and the technological environment. Looking at new tendencies within interaction design and ubiquitous computing to see...

  3. Commercial state-sponsored bodies

    OpenAIRE

    Walsh, Brendan M.

    1987-01-01

    The commercial state-sponsered bodies comprise an important element of economic activity in Ireland. The Exchequer makes a substantial financtial contribution to these bodies. In this article the author traces the origins and rationale of the commercial state bodies, examines their performance and discusses some major policy issues arising from their operations. non-peer-reviewed

  4. Exploring Feminist Women's Body Consciousness

    Science.gov (United States)

    Rubin, Lisa R.; Nemeroff, Carol J.; Russo, Nancy Felipe

    2004-01-01

    In a qualitative investigation of young feminists' experience of body consciousness, 25 feminist women each participated in one of 6 focus groups examining the ways they experienced body image and negotiated cultural messages about women's appearance. Participants described their experience with objectification and its impact on their body image,…

  5. Body language in English Teaching

    Institute of Scientific and Technical Information of China (English)

    任竞竞

    2009-01-01

    In this article, the characteristics and functions of body language are expounded, emphasizing that the proper use of body language in English teaching, including facial expression, guestures and body distance. They can enrich the content of English teaching, diversify teaching method and add vividness to the teaching to achieve better results .

  6. Body Image Concerns

    DEFF Research Database (Denmark)

    El Ansari, Walid; Dibba, Emily; Stock, Christiane

    2014-01-01

    AIMS: This cross-sectional study aimed to determine the socio-demographic, lifestyle and well-being variables that are associated with body image concerns (BIC) and whether these associations differed between female and male students. METHODS: A cross-sectional survey; 3,706 undergraduate students...... for the association between four increasing levels of BIC as the dependent variables (no BIC, mild BIC, moderate BIC and marked BIC) on the one hand, and the socio-demographic (gender, age, year at university), lifestyle (physical activity, nutrition) and mental well-being variables (quality of life, perceived stress...... at university. Females' BICs were exclusively associated with low perceived health, higher perceived stress, studies in general as a stressor, and low physical activity. In contrast, males' BIC were found to be exclusively associated with low quality of life and with older age. CONCLUSIONS: Health promoting...

  7. Quantum many body systems

    Energy Technology Data Exchange (ETDEWEB)

    Rivasseau, Vincent [Paris-Sud Univ. Orsay (France). Laboratoire de Physique Theorique; Seiringer, Robert [McGill Univ., Montreal, QC (Canada). Dept. of Mathematics and Statistics; Solovej, Jan Philip [Copenhagen Univ. (Denmark). Dept. of Mathematics; Spencer, Thomas [Institute for Advanced Study, Princeton, NJ (United States). School of Mathematics

    2012-11-01

    The book is based on the lectures given at the CIME school ''Quantum many body systems'' held in the summer of 2010. It provides a tutorial introduction to recent advances in the mathematics of interacting systems, written by four leading experts in the field: V. Rivasseau illustrates the applications of constructive Quantum Field Theory to 2D interacting electrons and their relation to quantum gravity; R. Seiringer describes a proof of Bose-Einstein condensation in the Gross-Pitaevski limit and explains the effects of rotating traps and the emergence of lattices of quantized vortices; J.-P. Solovej gives an introduction to the theory of quantum Coulomb systems and to the functional analytic methods used to prove their thermodynamic stability; finally, T. Spencer explains the supersymmetric approach to Anderson localization and its relation to the theory of random matrices. All the lectures are characterized by their mathematical rigor combined with physical insights.

  8. Impact between deformable bodies

    International Nuclear Information System (INIS)

    The bodies are represented by constant strain finite elements so that the element internal forces can most easily be calculated, especially after yielding has taken place when the stress and strain increments are related in accordance with the Prandtl-Reuss theory. In the case of axisymmetrical problems triangular axisymmetrical elements are used whose properties are approximately calculated by sampling at the centroid of the cross-section. The external applied forces arise from the impact and contact forces at the interfaces, and the inertia forces are obtained from lumped mass matrices. The equation of motion is solved by a central difference explicit scheme in small incremental time steps. This enables the stress propagation as well as the history of plastic deformation in the bodies to be traced throughout the duration of impact. The material law is idealised to be piecewise linear, with an initial elastic portion followed by one linear hardening segment. Perfect plasticity (zero hardening) can also be allowed. A simple procedure deals with the case of loading from an elastic initial state to a final plastic state in one time step. The program has been applied to the investigation of a number of axisymmetrical problems. The three dimensional version of the program is now being coded. Examples: impact of a falling fuel stringer in a storage tube; impact of a cylinder on a rigid boundary; supported circular plate loaded by uniformly distributed impulses; impact of a non-return valve in a pipe rupture; impact of a cylindrical fuel-waste flask; impact of a conical missile on a rigid surface. (orig./HP)

  9. Human body communication performance simulations

    OpenAIRE

    Mufti, H. (Haseeb)

    2016-01-01

    Human Body Communication (HBC) is a novel communication method between devices which use human body as a transmission medium. This idea is mostly based on the concept of wireless biomedical monitoring system. The on-body sensor nodes can monitor vital signs of a human body and use the body as a transmission medium. This technology is convenient for long durations of clinical monitoring with the option of more mobility and freedom for the user. In this thesis, IEEE 802.15.6-2012 phy...

  10. The Body as a Pulse

    Directory of Open Access Journals (Sweden)

    Flavia Liberman

    2010-01-01

    Full Text Available The body is the focus of many studies and interventions. Some paradigms conceptualize the body only in relation to its motor-sensory characteristics, while others prioritize its psychological dimensions. With the aim of contributing towards formulating other perspectives within this field, some aspects of Stanley Keleman and Regina Favre's conceptualization of the body are presented here. Starting from clinical situations during seminar groups, we can take the body to be a multifaceted multimedia pulse that is continually [de]constructed through encounters. Together with the author's clinical experiences as an occupational therapist and teacher or undergraduates, these conceptualizations serve as a guide to clinical practice that is thought out, constructed and balanced by the body, using body approaches to promote encounters molded by affections and events, in an attempt to create bodies capable of sustaining the lived intensity of experiences, and which enable self-observation, closeness to other people and production of singularities.

  11. Body checking behaviors in men.

    Science.gov (United States)

    Walker, D Catherine; Anderson, Drew A; Hildebrandt, Thomas

    2009-06-01

    Males have been facing increasing pressure from the media to attain a lean, muscular physique, and are at risk for body dissatisfaction, disturbed eating and exercise behaviors, and abuse of appearance- and performance-enhancing drugs (APEDs). The aim of the current study was to examine the relationship between body checking and mood, symptoms of muscle dysmorphia, importance of shape and weight, and APED use in undergraduate males. Body checking in males was correlated with weight and shape concern, symptoms of muscle dysmorphia, depression, negative affect, and APED use. Body checking predicted APED use and uniquely accounted for the largest amount of variance in Muscle Dysmorphic Disorder Inventory (MDDI) scores (16%). Findings support the view that body checking is an important construct in male body image, muscle dysmorphia, and body change strategies and suggest a need for further research. PMID:19482568

  12. Discinesia ciliar primária: considerações sobre seis casos da síndrome de Kartagener Primary ciliary dyskinesia: considerations regarding six cases of Kartagener syndrome

    Directory of Open Access Journals (Sweden)

    Hugo Alejandro Vega Ortega

    2007-10-01

    Full Text Available A discinesia ciliar primária (DCP, anteriormente conhecida como síndrome dos cílios imóveis, é uma doença hereditária autossômica recessiva que inclui vários padrões de defeitos em sua ultra-estrutura ciliar. Sua forma clínica mais grave é a síndrome de Kartagener (SK, a qual é encontrada em 50% dos casos de DCP. A DCP causa deficiência ou mesmo estase no transporte de secreções em todo o trato respiratório, favorecendo a proliferação de vírus e bactérias. Sua incidência varia de 1:20.000 a 1:60.000. Como conseqüência, os pacientes apresentam infecções crônicas e repetidas desde a infância e geralmente são portadores de bronquite, pneumonia, hemoptise, sinusite e infertilidade. As bronquiectasias e outras infecções crônicas podem ser o resultado final das alterações irreversíveis dos brônquios, podendo progredir para cor pulmonale crônico e suas conseqüências. Somente a metade dos pacientes afetados pela DCP apresenta todos os sintomas, condição denominada SK completa; no restante, não ocorre situs inversus, condição denominada SK incompleta. O diagnóstico é feito com base no quadro clínico e confirmado por meio da microscopia eletrônica de transmissão. Como não há tratamento especifico para a DCP, recomenda-se que, tão logo seja feito o diagnóstico, as infecções secundárias sejam tratadas com antibióticos potentes e medidas profiláticas sejam adotadas. Neste trabalho, relatamos seis casos de DCP (cinco casos de SK completa e um caso de SK incompleta e revisamos a literatura sobre o assunto, tendo como foco os aspectos diagnósticos, terapêuticos e clínicos desta doença.Primary ciliary dyskinesia (PCD, previously known as immotile cilia syndrome, is an autosomal recessive hereditary disease that includes various patterns of ciliary ultrastructural defects. The most serious form is Kartagener syndrome (KS, which accounts for 50% of all cases of PCD. The incidence of PCD ranges from 1

  13. The Becoming of Bodies: Girls, media effects, and body image

    OpenAIRE

    Coleman, Rebecca

    2008-01-01

    The relations between women's bodies and images have long interested and occupied feminist theoretical and empirical work. Recently, much feminist research has focused on the relations between girls' and young women's bodies and images in “the media.” Underpinning much of this research, I argue, is an oppositional model of subject/object onto which bodies and images are mapped. Developing Deleuze's concept of becoming and exploring my own research with a small number of white British teenage ...

  14. The -Curvature Images of Convex Bodies and -Projection Bodies

    Indian Academy of Sciences (India)

    Songjun Lv; Gangsong Leng

    2008-08-01

    Associated with the -curvature image defined by Lutwak, some inequalities for extended mixed -affine surface areas of convex bodies and the support functions of -projection bodies are established. As a natural extension of a result due to Lutwak, an -type affine isoperimetric inequality, whose special cases are -Busemann–Petty centroid inequality and -affine projection inequality, respectively, is established. Some -mixed volume inequalities involving -projection bodies are also established.

  15. Métodos comparativos para recomposição de áreas de mata ciliar avaliados por análise longitudinal = Comparative methods for ciliary forest rearrangement evaluated by longitudinal analysis

    Directory of Open Access Journals (Sweden)

    Alessandra Costa Carrito Bobato

    2008-01-01

    Full Text Available Foram avaliados três métodos de distribuição de mudas para recomposição artificial de área de mata ciliar estudando-se a variação do crescimento da estatura e diâmetro durante seis períodos em um ano, das espécies Schinus terbinthifolia Raddi–aroeira ePeltophorum dubium Taub–canafístula. Os métodos estudados foram: mudas distribuídas ao acaso em espaçamento de 2 x 2 m; mudas distribuídas individualmente ao acaso; mudas distribuídas ao acaso em agrupamento três a três, com espaçamento de 1 a 1,5 m. O crescimento da estatura e do diâmetro das espécies estudadas está diretamente relacionado com os períodos em estudo, o tratamento de recomposição e as espécies. O tratamento mudas distribuídas ao acaso individualmente foi o que apresentou melhor crescimento médio do diâmetro.This trial aimed at evaluating three methods to distribute seedlingsfrom Schinus terbinthifolia and Peltophorum dubium species for rearrangement of ciliary forests. Their growth changes in height and diameter were used as parameters during six terms in one year. The studied methods were: seedlings randomized distributed in 2 x 2 m; seedlings individually distributed in a randomized design and seedlings randomized distributed in groups by three, using a 1 to 1.5 m row distance. The growth regarding diameter and height of the observed species is straightly related to the studied periods, rearrangement treatment and species. As a conclusion, it was registered that the seedling treatment individually distributed in a randomized design was the best answer regarding the average growth ofdiameter.

  16. The relation between body semantics and spatial body representations.

    Science.gov (United States)

    van Elk, Michiel; Blanke, Olaf

    2011-11-01

    The present study addressed the relation between body semantics (i.e. semantic knowledge about the human body) and spatial body representations, by presenting participants with word pairs, one below the other, referring to body parts. The spatial position of the word pairs could be congruent (e.g. EYE / MOUTH) or incongruent (MOUTH / EYE) with respect to the spatial position of the words' referents. In addition, the spatial distance between the words' referents was varied, resulting in word pairs referring to body parts that are close (e.g. EYE / MOUTH) or far in space (e.g. EYE / FOOT). A spatial congruency effect was observed when subjects made an iconicity judgment (Experiments 2 and 3) but not when making a semantic relatedness judgment (Experiment 1). In addition, when making a semantic relatedness judgment (Experiment 1) reaction times increased with increased distance between the body parts but when making an iconicity judgment (Experiments 2 and 3) reaction times decreased with increased distance. These findings suggest that the processing of body-semantics results in the activation of a detailed visuo-spatial body representation that is modulated by the specific task requirements. We discuss these new data with respect to theories of embodied cognition and body semantics.

  17. Body fat mass in normal weight subjects

    OpenAIRE

    Stokić Edita J.; Srdić Biljana; Peter Andrea; Ivković-Lazar Tatjana A.

    2002-01-01

    Obesity is characterized by excessive body fat accumulation which may lead to serious health problems and complications. Body mass index is the most optimal parameter to evaluate the level of nutritional status and diagnose obesity. However, modern techniques studying body composition can more accurately determine whether the gain of body weight was on the account of body fat, lean body mass or total body water. If one's body mass index is in the range of normal values but the amount of body ...

  18. Stereotactic body radiation therapy

    Energy Technology Data Exchange (ETDEWEB)

    Lo, Simon S. [Univ. Hospitals Seidman Cancer Center, Cleveland, OH (United States). Dept. of Radiation Oncology; Case Western Reserve Univ., Cleveland, OH (United States). Case Comprehensive Cancer Center; Teh, Bin S. [The Methodist Hospital Cancer Center and Research Institute, Houston, TX (United States). Weill Cornell Medical College; Lu, Jiade J. [National Univ. of Singapore (Singapore). Dept. of Radiation Oncology; Schefter, Tracey E. (eds.) [Colorado Univ., Aurora, CO (United States). Dept. of Radiation Oncology

    2012-11-01

    Comprehensive an up-to-date account of the physical/technological, biological, and clinical aspects of SBRT. Examines in detail retrospective studies and prospective clinical trials for various organ sites from around the world. Written by world-renowned experts in SBRT from North America, Asia and Europe. Stereotactic body radiation therapy (SBRT) has emerged as an innovative treatment for various primary and metastatic cancers, and the past five years have witnessed a quantum leap in its use. This book provides a comprehensive and up-to-date account of the physical/technological, biological, and clinical aspects of SBRT. It will serve as a detailed resource for this rapidly developing treatment modality. The organ sites covered include lung, liver, spine, pancreas, prostate, adrenal, head and neck, and female reproductive tract. Retrospective studies and prospective clinical trials on SBRT for various organ sites from around the world are examined, and toxicities and normal tissue constraints are discussed. This book features unique insights from world-renowned experts in SBRT from North America, Asia, and Europe. It will be necessary reading for radiation oncologists, radiation oncology residents and fellows, medical physicists, medical physics residents, medical oncologists, surgical oncologists, and cancer scientists.

  19. Bodies, Spaces, Voices, Silences

    Directory of Open Access Journals (Sweden)

    Donatella Mazzoleni

    2013-07-01

    Full Text Available A good architecture should not only allow functional, formal and technical quality for urban spaces, but also let the voice of the city be perceived, listened, enjoyed. Every city has got its specific sound identity, or “ISO” (R. O. Benenzon, made up of a complex texture of background noises and fluctuation of sound figures emerging and disappearing in a game of continuous fadings. For instance, the ISO of Naples is characterized by a spread need of hearing the sound return of one’s/others voices, by a hate of silence. Cities may fall ill: illness from noise, within super-crowded neighbourhoods, or illness from silence, in the forced isolation of peripheries. The proposal of an urban music therapy denotes an unpublished and innovative enlarged interdisciplinary research path, where architecture, music, medicine, psychology, communication science may converge, in order to work for rebalancing spaces and relation life of the urban collectivity, through the care of body and sound dimensions.

  20. Move your body!

    Institute of Scientific and Technical Information of China (English)

    撒哈拉

    2005-01-01

    还怀念当年跳舞毯风靡的时光吗?左脚、右脚向前.向后,在不经意间展示你炫目的舞步。而最近PS2平台上的《EyeToy》让我们又接触到了一种需要游戏者舞动身体来参与的摄像头游戏.既玩了游戏又锻炼了身体.而昂达Eyebob摄像头让PC用户也能参与其中。想要玩玩摄像头游戏.现在不用转投Ps2的阵营.也不用改造你的硬件.只要你拥有一款摄像头.你就可以和家人与朋友轻松享受游戏的乐趣。来吧!Come on! Move your body now!

  1. 小切口人工晶状体睫状沟悬吊术在玻璃体切割术后无晶状体囊膜眼应用%Application of intraocular lens implantation with suture fixation at ciliary sulcus in aphakia after vitrectomy through small incision

    Institute of Scientific and Technical Information of China (English)

    韩非; 吴燕; 段烈英; 刘恒; 罗涛; 蒋炜

    2012-01-01

    Objective To investigate the application of intraocular lens implantation with suture fixation at ciliary sulcus in aphakia after vitrectomy through small incision.Methods Intraocular lens implantation with suture fixation at ciliary sulcus was performed in 25 patients of aphakia and without capsule after vitrectomy.All the cases were followed up 1-6 months postoperatively.Results The visual acuity of all operated eyes was better than or equal to that of pre-operative eyes.No severe complication was found.Conclusions Intraocular lens implantation with suture fixation at ciliary sulcus through small incision is an effective method to aphakia after vitrectomy.%目的 探讨小切口人工晶状体睫状沟悬吊术在玻璃体切割术后无晶状体囊膜眼中的应用情况.方法 在球后 、表面麻醉下给25例(25只眼)玻璃体切割术后无晶状体且无囊膜眼行角膜缘小切口人工晶状体睫状沟悬吊术.术后随访1~6月.结果 25只眼术后视力均达到或超过术前矫正视力,无严重并发症.结论 小切口人工晶状体睫状沟悬吊术治疗玻璃体切割术后无晶状体囊膜眼的效果确切.

  2. Gravitational N-body Simulations

    CERN Document Server

    Trenti, M

    2008-01-01

    Gravitational N-body simulations, that is numerical solutions of the equations of motions for N particles interacting gravitationally, are widely used tools in astrophysics, with applications from few body or solar system like systems all the way up to galactic and cosmological scales. In this article we present a summary review of the field highlighting the main methods for N-body simulations and the astrophysical context in which they are usually applied.

  3. Body fluid identification in forensics

    OpenAIRE

    Ja Hyun An1, Kyoung-Jin Shin1,2, Woo Ick Yang1 & Hwan Young Lee1,2,*

    2012-01-01

    At a crime scene can give important insights into crime scenereconstruction by supporting a link between sample donorsand actual criminal acts. For more than a century, numeroustypes of body fluid identification methods have beendeveloped, such as chemical tests, immunological tests,protein catalytic activity tests, spectroscopic methods andmicroscopy. However, these conventional body fluididentification methods are mostly presumptive, and are carriedout for only one body fluid at a time. The...

  4. EFFICIENCY OF TAX BODIES' PERFORMANCE

    OpenAIRE

    Fedorovskaya, M.

    2011-01-01

    The problems of assessment of tax bodies' activities efficiency which tax administration faces are touched upon. Suggestions made provide an opportunity for bodies of legislative and executive power to take specific measures aimed at enhancing the system of tax administration in the country. Suggested criteria for assessing tax bodies' performance can be put into practice by tax authorities. A new approach to aspects of solving tasks of control over tax compliance, preventing and excluding ta...

  5. 3 - Dimensional Body Measurement Technology

    Institute of Scientific and Technical Information of China (English)

    ZHOU Xu-dong; LI Yan-mei

    2002-01-01

    3 - dimensional body measurement technology, the basis of developing high technology in industry, accelerates digital development of aplparel industry. This paper briefly introduces the history of 3 - dimensional body measurement technology, and recounts the principle and primary structure of some types of 3 - dimensional automatic body measurement system. With this understanding, it discusses prospect of 3- dimensional CAD and virtual technology used in apparel industry.

  6. Body checking behaviors in men

    OpenAIRE

    Walker, D. Catherine; Anderson, Drew A.; Hildebrandt, Thomas

    2009-01-01

    Males have been facing increasing pressure from the media to attain a lean, muscular physique, and are at risk for body dissatisfaction, disturbed eating and exercise behaviors, and abuse of appearance- and performance-enhancing drugs (APEDs). The aim of the current study was to examine the relationship between body checking and mood, symptoms of muscle dysmorphia, importance of shape and weight, and APED use in undergraduate males. Body checking in males was correlated with weight and shape ...

  7. The functional architecture of the human body: assessing body representation by sorting body parts and activities.

    Science.gov (United States)

    Bläsing, Bettina; Schack, Thomas; Brugger, Peter

    2010-05-01

    We investigated mental representations of body parts and body-related activities in two subjects with congenitally absent limbs (one with, the other without phantom sensations), a wheelchair sports group of paraplegic participants, and two groups of participants with intact limbs. To analyse mental representation structures, we applied Structure Dimensional Analysis. Verbal labels indicating body parts and related activities were presented in randomized lists that had to be sorted according to a hierarchical splitting paradigm. Participants were required to group the items according to whether or not they were considered related, based on their own body perception. Results of the groups of physically intact and paraplegic participants revealed separate clusters for the lower body, upper body, fingers and head. The participant with congenital phantom limbs also showed a clear separation between upper and lower body (but not between fingers and hands). In the participant without phantom sensations of the absent arms, no such modularity emerged, but the specific practice of his right foot in communication and daily routines was reflected. Sorting verbal labels of body parts and activities appears a useful method to assess body representation in individuals with special body anatomy or function and leads to conclusions largely compatible with other assessment procedures.

  8. The Body and the Beautiful: Health, Attractiveness and Body Composition in Men's and Women's Bodies.

    Science.gov (United States)

    Brierley, Mary-Ellen; Brooks, Kevin R; Mond, Jonathan; Stevenson, Richard J; Stephen, Ian D

    2016-01-01

    The dominant evolutionary theory of physical attraction posits that attractiveness reflects physiological health, and attraction is a mechanism for identifying a healthy mate. Previous studies have found that perceptions of the healthiest body mass index (weight scaled for height; BMI) for women are close to healthy BMI guidelines, while the most attractive BMI is significantly lower, possibly pointing to an influence of sociocultural factors in determining attractive BMI. However, less is known about ideal body size for men. Further, research has not addressed the role of body fat and muscle, which have distinct relationships with health and are conflated in BMI, in determining perceived health and attractiveness. Here, we hypothesised that, if attractiveness reflects physiological health, the most attractive and healthy appearing body composition should be in line with physiologically healthy body composition. Thirty female and 33 male observers were instructed to manipulate 15 female and 15 male body images in terms of their fat and muscle to optimise perceived health and, separately, attractiveness. Observers were unaware that they were manipulating the muscle and fat content of bodies. The most attractive apparent fat mass for female bodies was significantly lower than the healthiest appearing fat mass (and was lower than the physiologically healthy range), with no significant difference for muscle mass. The optimal fat and muscle mass for men's bodies was in line with the healthy range. Male observers preferred a significantly lower overall male body mass than did female observers. While the body fat and muscle associated with healthy and attractive appearance is broadly in line with physiologically healthy values, deviations from this pattern suggest that future research should examine a possible role for internalization of body ideals in influencing perceptions of attractive body composition, particularly in women. PMID:27257677

  9. The Body and the Beautiful: Health, Attractiveness and Body Composition in Men's and Women's Bodies.

    Directory of Open Access Journals (Sweden)

    Mary-Ellen Brierley

    Full Text Available The dominant evolutionary theory of physical attraction posits that attractiveness reflects physiological health, and attraction is a mechanism for identifying a healthy mate. Previous studies have found that perceptions of the healthiest body mass index (weight scaled for height; BMI for women are close to healthy BMI guidelines, while the most attractive BMI is significantly lower, possibly pointing to an influence of sociocultural factors in determining attractive BMI. However, less is known about ideal body size for men. Further, research has not addressed the role of body fat and muscle, which have distinct relationships with health and are conflated in BMI, in determining perceived health and attractiveness. Here, we hypothesised that, if attractiveness reflects physiological health, the most attractive and healthy appearing body composition should be in line with physiologically healthy body composition. Thirty female and 33 male observers were instructed to manipulate 15 female and 15 male body images in terms of their fat and muscle to optimise perceived health and, separately, attractiveness. Observers were unaware that they were manipulating the muscle and fat content of bodies. The most attractive apparent fat mass for female bodies was significantly lower than the healthiest appearing fat mass (and was lower than the physiologically healthy range, with no significant difference for muscle mass. The optimal fat and muscle mass for men's bodies was in line with the healthy range. Male observers preferred a significantly lower overall male body mass than did female observers. While the body fat and muscle associated with healthy and attractive appearance is broadly in line with physiologically healthy values, deviations from this pattern suggest that future research should examine a possible role for internalization of body ideals in influencing perceptions of attractive body composition, particularly in women.

  10. A Respiration Rate Body Sensor

    OpenAIRE

    Viktor Avbelj; Aleksandra Rashkovska; Roman Trobec

    2012-01-01

    We propose a new body sensor for extracting the respiration rate based on the amplitude changes in the body surface potential differences between two proximal body electrodes. The sensor could be designed as a plaster-like reusable unit that can be easily fixed onto the surface of the body. It could be equipped either with a sufficiently large memory for storing the measured data or with a low-power radio system that can transmit the measured data to a gateway for further processing. We explo...

  11. [Neuroanatomy of Isolated Body Lateropulsion].

    Science.gov (United States)

    Nakazato, Yoshihiko; Tamura, Naotoshi; Ikeda, Kei; Tanaka, Ai; Yamamoto, Toshimasa

    2016-03-01

    Axial body lateropulsion, a phenomenon where the body is pulled toward the side of the lesion, with tendency of falling down, is the well-known transient feature of lateral medullary syndrome. In some cases, axial body lateropulsion occurs without vestibular and cerebellar symptoms (isolated body lateropulsion:[iBL]). Patients with iBL have a lesion located in the spinocerebellar tract, descending lateral vestibulospinal tract, vestibulo-thalamic pathway, dentatorubrothalamic pathway, or thalamocortical fascicle. This review deals with the anatomic basis and clinical significance of iBL. PMID:27001775

  12. Physics of the Human Body

    CERN Document Server

    Herman, Irving P

    2007-01-01

    Physics of the Human Body comprehensively addresses the physical and engineering aspects of human physiology by using and building on first-year college physics and mathematics. Topics include the mechanics of the static body and the body in motion, the materials properties of the body, muscles in the body, the energetics of body metabolism, fluid flow in the cardiovascular and respiratory systems, the acoustics of sound waves in speaking and hearing, vision and the optics of the eye, the electrical properties of the body, and the basic engineering principles of feedback and control in regulating all aspects of function. The goal of this text is to understand physical issues concerning the human body, in part by developing and then using simple and subsequently more refined models of the macrophysics of the human body. Many chapters include a brief review of the necessary physical principles. There are problems at the end of each chapter; solutions to selected problems are also provided. This text is geared t...

  13. Body composition in clinical practice.

    Science.gov (United States)

    Andreoli, Angela; Garaci, Francesco; Cafarelli, Francesco Pio; Guglielmi, Giuseppe

    2016-08-01

    Nutritional status is the results of nutrients intake, absorption and utilization, able to influence physiological and pathological conditions. Nutritional status can be measured for individuals with different techniques, such as CT Body Composition, quantitative Magnetic Resonance Imaging, Ultrasound, Dual-Energy X-Ray Absorptiometry and Bioimpendance. Because obesity is becoming a worldwide epidemic, there is an increasing interest in the study of body composition to monitor conditions and delay in development of obesity-related diseases. The emergence of these evidence demonstrates the need of standard assessment of nutritional status based on body weight changes, playing an important role in several clinical setting, such as in quantitative measurement of tissues and their fluctuations in body composition, in survival rate, in pathologic condition and illnesses. Since body mass index has been shown to be an imprecise measurement of fat-free and fat mass, body cell mass and fluids, providing no information if weight changes, consequently there is the need to find a better way to evaluate body composition, in order to assess fat-free and fat mass with weight gain and loss, and during ageing. Monitoring body composition can be very useful for nutritional and medical interventional. This review is focused on the use of Body Composition in Clinical Practice. PMID:26971404

  14. Body composition in clinical practice.

    Science.gov (United States)

    Andreoli, Angela; Garaci, Francesco; Cafarelli, Francesco Pio; Guglielmi, Giuseppe

    2016-08-01

    Nutritional status is the results of nutrients intake, absorption and utilization, able to influence physiological and pathological conditions. Nutritional status can be measured for individuals with different techniques, such as CT Body Composition, quantitative Magnetic Resonance Imaging, Ultrasound, Dual-Energy X-Ray Absorptiometry and Bioimpendance. Because obesity is becoming a worldwide epidemic, there is an increasing interest in the study of body composition to monitor conditions and delay in development of obesity-related diseases. The emergence of these evidence demonstrates the need of standard assessment of nutritional status based on body weight changes, playing an important role in several clinical setting, such as in quantitative measurement of tissues and their fluctuations in body composition, in survival rate, in pathologic condition and illnesses. Since body mass index has been shown to be an imprecise measurement of fat-free and fat mass, body cell mass and fluids, providing no information if weight changes, consequently there is the need to find a better way to evaluate body composition, in order to assess fat-free and fat mass with weight gain and loss, and during ageing. Monitoring body composition can be very useful for nutritional and medical interventional. This review is focused on the use of Body Composition in Clinical Practice.

  15. Body enhancement : body images, vulnerability and moral responsibility

    NARCIS (Netherlands)

    den Dikken, A.

    2011-01-01

    The objective of this explorative study is to show that it is highly relevant to integrate cultural and personal body images into the ethical debate on human enhancement. The current debate has little attention for the motivations to make use of technology to alter the human body, such as cultural i

  16. Body Talk: Body Image Commentary on Queerty.com.

    Science.gov (United States)

    Schwartz, Joseph; Grimm, Josh

    2016-08-01

    In this study, we conducted a content analysis of 243 photographic images of men published on the gay male-oriented blog Queerty.com. We also analyzed 435 user-generated comments from a randomly selected 1-year sample. Focusing on images' body types, we found that the range of body types featured on the blog was quite narrow-the vast majority of images had very low levels of body fat and very high levels of muscularity. Users' body image-related comments typically endorsed and celebrated images; critiques of images were comparatively rare. Perspectives from objectification theory and social comparison theory suggest that the images and commentary found on the blog likely reinforce unhealthy body image in gay male communities. PMID:26849832

  17. Physics of the human body

    CERN Document Server

    Herman, Irving P

    2016-01-01

    This book comprehensively addresses the physics and engineering aspects of human physiology by using and building on first-year college physics and mathematics. Topics include the mechanics of the static body and the body in motion, the mechanical properties of the body, muscles in the body, the energetics of body metabolism, fluid flow in the cardiovascular and respiratory systems, the acoustics of sound waves in speaking and hearing, vision and the optics of the eye, the electrical properties of the body, and the basic engineering principles of feedback and control in regulating all aspects of function. The goal of this text is to clearly explain the physics issues concerning the human body, in part by developing and then using simple and subsequently more refined models of the macrophysics of the human body. Many chapters include a brief review of the underlying physics. There are problems at the end of each chapter; solutions to selected problems are also provided. This second edition enhances the treat...

  18. Foreign bodies in gastrointestinal tract

    Directory of Open Access Journals (Sweden)

    Ayşe Kefeli

    2014-03-01

    Full Text Available Objective: Ingested foreign bodies in gastrointestinal tract are a common event which can cause serious morbidity and mortality in the children and adult population. For this reason, early diagnosis and treatment are crucial for preventing these life threatening complications. In this study, we aimed to analyze the characteristics of the patients with upper gastrointestinal foreign bodies that were treated in our department. Methods: Patients diagnosed with upper gastrointestinal foreign bodies who were admitted to our hospital between February 2010 and August2013 were evaluated retrospectively. The data regarding their age, gender, clinical profile, type and localization of the esophageal foreign body, performed endoscopic procedure and initial symptoms of the patients were noted and analyzed statistically. Results: Thirty-eight patients with a diagnosis of gastrointestinal foreign body were included in this study. Of these patients, 21 were male and 17 were female. The youngest patient was 17 years old and the oldest patient was 79 years old. Most of the foreign bodies (%55.3 detected in the stomach. Food waste and metallic objects in 21 and 16 patients respectively. The most common complaint was dysphagia (%50. After endoscopic intervention three of the patients were directed to surgery. Conclusion: Early recognition and treatment of gastrointestinal foreign bodies is important as their complications are life threatening. The best method of removal of foreign bodies is controversial. Early management with upper gastrointestinal endoscopy is the most efficient and safe treatment method in current conditions.

  19. Euler's Three-Body Problem.

    Science.gov (United States)

    Wild, Walter J.

    1980-01-01

    Discusses the simplest three-body problem, known as Euler's problem. The article, intended for students in the undergraduate mathematics and physics curricula, shows how the complex equations for a specific three-body problem can be solved on a small calculator. (HM)

  20. INEQUALITIES FOR MIXED INTERSECTION BODIES

    Institute of Scientific and Technical Information of China (English)

    YUAN SHUFENG; LENG GANGSONG

    2005-01-01

    In this paper, some properties of mixed intersection bodies are given, and inequalities from the dual Brunn-Minkowski theory (such as the dual Minkowski inequality, the dual Aleksandrov-Fenchel inequalities and the. dual Brunn-Minkowski inequalities) are established for mixed intersection bodies.

  1. Guy's Guide to Body Image

    Science.gov (United States)

    ... 2 • 3 • 4 For Teens For Kids For Parents MORE ON THIS TOPIC Body Dysmorphic Disorder Are Steroids Worth the Risk? Delayed Puberty How Can I Improve My Self-Esteem? Body Image and Self-Esteem Help! Is This ...

  2. Re-Educating the Body

    Science.gov (United States)

    Holst, Jonas

    2013-01-01

    This article means to investigate the philosophical concept of human embodiment in relation to physical education. As human beings not only do we have a body that we can control, but we "are" our body and live embodied in the world, as the German thinker, Helmuth Plessner, puts it in one of his many contributions to the philosophical…

  3. Body Language in the Classroom

    Science.gov (United States)

    Miller, Patrick W.

    2005-01-01

    Ralph Waldo Emerson and Mae West would seem to have little in common, but there is one thing they both understood--the importance of body language. Educators, psychologists, anthropologists and sociologists define body language or nonverbal communication as communication without words. It includes overt behaviors such as facial expressions, eye…

  4. Heritability of adult body height

    DEFF Research Database (Denmark)

    Silventoinen, Karri; Sammalisto, Sampo; Perola, Markus;

    2003-01-01

    A major component of variation in body height is due to genetic differences, but environmental factors have a substantial contributory effect. In this study we aimed to analyse whether the genetic architecture of body height varies between affluent western societies. We analysed twin data from ei...

  5. Migrating foreign body from hypopharynx

    DEFF Research Database (Denmark)

    Mowinckel, Marius Storm; Charabi, Birgitte Wittenborg

    2014-01-01

    In this case report we present a 20-month-old girl with a migrating foreign body, a "smiley" sticker, that migrated from hypopharynx to surrounding tissue and created an abscess with a fistula, one year after ingestion. The foreign body was removed without difficulty under general anaesthesia, and...

  6. Skin Pedagogies and Abject Bodies

    Science.gov (United States)

    Kenway, Jane; Bullen, Elizabeth

    2011-01-01

    How does the beauty industry "narrate the skin"? What does it teach women from different cultural groups about the female body? How does skin function as a site where female subjection and abjection are produced and reproduced? In this paper we examine the skin industry pointing to its extreme commodification of the female body and to the…

  7. Magnetic fields of rotating bodies

    International Nuclear Information System (INIS)

    After a short historical review of the magnetism of rotating bodies a new model, based on Stochastic Electrodynamics, is briefly presented. It is shown how the theory of cooperative phenomena applies to this model. The outcome of the theory is used to analyse results obtained in a laboratory experiment on the magnetism of rotating bodies

  8. Is My Body My Property?

    OpenAIRE

    Nawrocka, Marta

    2014-01-01

    The aim of the study is to evaluate human body law protection system. The author analyse selected issues related to the human body in order to evaluate it in its legal and ethical perspective. Presenting the topic the author refers to the Convention on Human Rights and Biomedicine and the Convention for the Protection of Human Rights and Dignity of the Human Being.

  9. [Obesity: stigmatization, discrimination, body image].

    Science.gov (United States)

    Kinzl, Johann F

    2016-03-01

    Obesity is a heterogeneous condition with multifactorial genesis (genetic predisposition, life-style, psychosocial situation), but there is a relatively homogeneous negative stereotype of obese individuals, because overweight and obesity are seen as self-inflicted disorders caused by physical inactivity and disorderd eating behavior. Obese individuals are confronted with far-reaching stigmatization and discrimination. Typical stereotypes are laziness, unattractiveness, work refusal. This negative image by the environment contributes to negative self-awareness and self-stigmatization, accompanied by a poor self-esteem and feelings of poor self-control and reduced self-efficacy, resulting in poor constructive coping strategies for overweight reduction. In addition, a disturbed body image combined with deep dissatisfaction with their own body is often found in many obese individuals. There is not always a close connection between body weight and body dissatisfaction. Young women and individuals with a binge eating disorder often show an increased body dissatisfaction as well. PMID:26883770

  10. Body fluid identification in forensics

    Directory of Open Access Journals (Sweden)

    Ja Hyun An1, Kyoung-Jin Shin1,2, Woo Ick Yang1 & Hwan Young Lee1,2,*

    2012-10-01

    Full Text Available At a crime scene can give important insights into crime scenereconstruction by supporting a link between sample donorsand actual criminal acts. For more than a century, numeroustypes of body fluid identification methods have beendeveloped, such as chemical tests, immunological tests,protein catalytic activity tests, spectroscopic methods andmicroscopy. However, these conventional body fluididentification methods are mostly presumptive, and are carriedout for only one body fluid at a time. Therefore, the use of amolecular genetics-based approach using RNA profiling orDNA methylation detection has been recently proposed tosupplant conventional body fluid identification methods.Several RNA markers and tDMRs (tissue-specific differentiallymethylated regions which are specific to forensically relevantbody fluids have been identified, and their specificities andsensitivities have been tested using various samples. In thisreview, we provide an overview of the present knowledge andthe most recent developments in forensic body fluididentification and discuss its possible practical application toforensic casework.

  11. Central control of body temperature.

    Science.gov (United States)

    Morrison, Shaun F

    2016-01-01

    Central neural circuits orchestrate the behavioral and autonomic repertoire that maintains body temperature during environmental temperature challenges and alters body temperature during the inflammatory response and behavioral states and in response to declining energy homeostasis. This review summarizes the central nervous system circuit mechanisms controlling the principal thermoeffectors for body temperature regulation: cutaneous vasoconstriction regulating heat loss and shivering and brown adipose tissue for thermogenesis. The activation of these thermoeffectors is regulated by parallel but distinct efferent pathways within the central nervous system that share a common peripheral thermal sensory input. The model for the neural circuit mechanism underlying central thermoregulatory control provides a useful platform for further understanding of the functional organization of central thermoregulation, for elucidating the hypothalamic circuitry and neurotransmitters involved in body temperature regulation, and for the discovery of novel therapeutic approaches to modulating body temperature and energy homeostasis. PMID:27239289

  12. Body surface area determined by whole-body CT scanning

    DEFF Research Database (Denmark)

    Villa, Chiara; Primeau, Charlotte; Hesse, Ulrik;

    2016-01-01

    Calculation of the estimated body surface area (BSA) by body height and weight has been a challenge in the past centuries due to lack of a well-documented gold standard. More recently, available techniques such as 3D laser surface scanning and CT scanning may be expected to quantify the BSA...... in an easier and more accurate way. This study provides the first comparison between BSA obtained from post-mortem whole-body CT scans and BSA calculated by nine predictive formulae. The sample consisted of 54 male cadavers ranging from 20 to 87 years old. 3D reconstructions were generated from CT scans using...

  13. Body Image and Eating Disorders

    Directory of Open Access Journals (Sweden)

    Janete Maximiano

    2014-10-01

    Full Text Available Eating disorders should be understood in a multidimensional perspective, emphasizing a biopsicossocial context. In these pathologies it`s the body, in the first instance, that reveals the disease, being in this way the target of the conflict, revealing a disturbed body experience and as a consequence a weak conception of their personal body image. The body image is conceptualised as a subjective image that the individuals form in their own mind, about their body, in relation with differ- ent contexts of life. The intent of the studies is to comprehend the level of body image disturbance, which have concluded that in the majority of the cases, significant changes on perceptive capacity of the patients do not exist. In this way it`s important to study in a more effective and qualitative way the affective and personal factors. The authors pretend with this bibliographic revision, make a research of body image assessment to the Eating Disorders (Anorexia Nervosa and Bulimia Nervosa, and to reflect which are the best ones to adapt for Portuguese reality.

  14. Pacemakers charging using body energy.

    Science.gov (United States)

    Bhatia, Dinesh; Bairagi, Sweeti; Goel, Sanat; Jangra, Manoj

    2010-01-01

    Life-saving medical implants like pacemakers and defibrillators face a big drawback that their batteries eventually run out and patients require frequent surgery to have these batteries replaced. With the advent of technology, alternatives can be provided for such surgeries. To power these devices, body energy harvesting techniques may be employed. Some of the power sources are patient's heartbeat, blood flow inside the vessels, movement of the body parts, and the body temperature (heat). Different types of sensors are employed, such as for sensing the energy from the heartbeat the piezoelectric and semiconducting coupled nanowires are used that convert the mechanical energy into electricity. Similarly, for sensing the blood flow energy, nanogenerators driven by ultrasonic waves are used that have the ability to directly convert the hydraulic energy in human body to electrical energy. Another consideration is to use body heat employing biothermal battery to generate electricity using multiple arrays of thermoelectric generators built into an implantable chip. These generators exploit the well-known thermocouple effect. For the biothermal device to work, it needs a 2°C temperature difference across it. But there are many parts of the body where a temperature difference of 5°C exists - typically in the few millimeters just below the skin, where it is planned to place this device. This study focuses on using body heat as an alternative energy source to recharge pacemaker batteries and other medical devices and prevent the possibility of life-risk during repeated surgery.

  15. Pacemakers charging using body energy

    Directory of Open Access Journals (Sweden)

    Dinesh Bhatia

    2010-01-01

    Full Text Available Life-saving medical implants like pacemakers and defibrillators face a big drawback that their batteries eventually run out and patients require frequent surgery to have these batteries replaced. With the advent of technology, alternatives can be provided for such surgeries. To power these devices, body energy harvesting techniques may be employed. Some of the power sources are patient′s heartbeat, blood flow inside the vessels, movement of the body parts, and the body temperature (heat. Different types of sensors are employed, such as for sensing the energy from the heartbeat the piezoelectric and semiconducting coupled nanowires are used that convert the mechanical energy into electricity. Similarly, for sensing the blood flow energy, nanogenerators driven by ultrasonic waves are used that have the ability to directly convert the hydraulic energy in human body to electrical energy. Another consideration is to use body heat employing biothermal battery to generate electricity using multiple arrays of thermoelectric generators built into an implantable chip. These generators exploit the well-known thermocouple effect. For the biothermal device to work, it needs a 2°C temperature difference across it. But there are many parts of the body where a temperature difference of 5°C exists - typically in the few millimeters just below the skin, where it is planned to place this device. This study focuses on using body heat as an alternative energy source to recharge pacemaker batteries and other medical devices and prevent the possibility of life-risk during repeated surgery.

  16. Body shape preferences: Associations with rater body shape and sociosexuality

    OpenAIRE

    Price, Michael E.; Nicholas Pound; James Dunn; Sian Hopkins; Jinsheng Kang

    2013-01-01

    Copyright @ 2013 Price et al. This article has been made available through the Brunel Open Access Publishing Fund. There is accumulating evidence of condition-dependent mate choice in many species, that is, individual preferences varying in strength according to the condition of the chooser. In humans, for example, people with more attractive faces/bodies, and who are higher in sociosexuality, exhibit stronger preferences for attractive traits in opposite-sex faces/bodies. However, prev...

  17. Uncommon, undeclared oesophageal foreign bodies.

    Science.gov (United States)

    Akenroye, M I; Osukoya, A T

    2012-01-01

    We report two cases of unusual and undeclared oesophageal foreign bodies. A small double-rounded calabash or bottle gourd Lagenaria siceraria, stuffed with traditional medicine designed to acquire spiritual power. A whole tricotyledonous kola nut Cola nitida also designed to make medicine to gain love from a woman after passing it out in stool. Each case presented with a sudden onset of total dysphagia and history of ingestion of foreign bodies was not volunteered by any despite direct questioning. Plain radiograph of the neck and chest in either case did not reveal presence of foreign body. Both were successfully removed through rigid oesophagoscopy. PMID:22718184

  18. Progress in computerized body tomography

    International Nuclear Information System (INIS)

    Since computerized tomography of the head has become well-established as a most important diagnostic tool in medicine, technical advance and scientific investigation has centered on CT of the remainder of the body. A proliferation of scientific papers covering many aspects of body scanning has occured, but the evidence is only slowly emerging to establish the indications, advantages, and cost effectiveness of computerized tomography below the head. An appraisal of the present status of computerized body tomography from viewpoint of the clinical radiologist is presented. 1977 planning recommendations for the western United States are cited. (orig.) 891 MG/orig. 892 MB

  19. Foreign body in the heart

    Institute of Scientific and Technical Information of China (English)

    LI Xin-min; ZHU Hong-yu; WANG Zhen-long; XU Zhao-hua

    2005-01-01

    @@ As an emergency, a foreign body in the heart should generally be removed as early as possible by surgical or non-surgical procedures in order to avoid any complications. Early treatment typically results in a better prognosis.

  20. The three-body problem

    CERN Document Server

    Musielak, Z E

    2015-01-01

    The three-body problem, which describes three masses interacting through Newtonian gravity without any restrictions imposed on the initial positions and velocities of these masses, has attracted the attention of many scientists for more than 300 years. In this paper, we present a review of the three-body problem in the context of both historical and modern developments. We describe the general and restricted (circular and elliptic) three-body problems, different analytical and numerical methods of finding solutions, methods for performing stability analysis, search for periodic orbits and resonances, and application of the results to some interesting astronomical and space dynamical settings. We also provide a brief presentation of the general and restricted relativistic three-body problems, and discuss their astronomical applications.

  1. Nonlinear Deformable-body Dynamics

    CERN Document Server

    Luo, Albert C J

    2010-01-01

    "Nonlinear Deformable-body Dynamics" mainly consists in a mathematical treatise of approximate theories for thin deformable bodies, including cables, beams, rods, webs, membranes, plates, and shells. The intent of the book is to stimulate more research in the area of nonlinear deformable-body dynamics not only because of the unsolved theoretical puzzles it presents but also because of its wide spectrum of applications. For instance, the theories for soft webs and rod-reinforced soft structures can be applied to biomechanics for DNA and living tissues, and the nonlinear theory of deformable bodies, based on the Kirchhoff assumptions, is a special case discussed. This book can serve as a reference work for researchers and a textbook for senior and postgraduate students in physics, mathematics, engineering and biophysics. Dr. Albert C.J. Luo is a Professor of Mechanical Engineering at Southern Illinois University, Edwardsville, IL, USA. Professor Luo is an internationally recognized scientist in the field of non...

  2. The Context of Body Art

    Directory of Open Access Journals (Sweden)

    Renata Clerici

    2011-10-01

    Full Text Available The present study analyses factors associated with the propensity for piercing and tattooing among adolescents, from a survey conducted in 2007 in high schools in the Veneto Region (Northeast Italy. After some descriptive analyses, multinomial logistic regression models were applied to identify what predicts the practice of, interest in, or indifference to piercing and tattooing. Results show gender differences in the approach to body modification: For girls, body art is considered a mainstream activity involving younger cohorts; piercing and tattooing become a way of increasing their own body satisfaction. For boys, factors connected with the propensity for these practices are a low cultural background and the importance given to physical appearance. For both boys and girls, a significant aspect in defining attitudes toward body art practices is the values system: Values such as enjoyment, beauty, and youth, are found to be closely connected with a high propensity for these practices.

  3. Intravascular "mulberry-like" bodies

    DEFF Research Database (Denmark)

    Sørensen, Flemming Brandt; Klebe, J G; Henriques, U V

    1988-01-01

    Intravascular "mulberry-like" bodies in a stillborn female infant with moderate maceration are reported. The histogenesis of these structures is discussed based on light-microscopic, immunohistochemical and ultrastructural findings. No demonstrable causal relation between the intravascular lesions...

  4. Hanford whole body counting manual

    International Nuclear Information System (INIS)

    This document, a reprint of the Whole Body Counting Manual, was compiled to train personnel, document operation procedures, and outline quality assurance procedures. The current manual contains information on: the location, availability, and scope of services of Hanford's whole body counting facilities; the administrative aspect of the whole body counting operation; Hanford's whole body counting facilities; the step-by-step procedure involved in the different types of in vivo measurements; the detectors, preamplifiers and amplifiers, and spectroscopy equipment; the quality assurance aspect of equipment calibration and recordkeeping; data processing, record storage, results verification, report preparation, count summaries, and unit cost accounting; and the topics of minimum detectable amount and measurement accuracy and precision. 12 refs., 13 tabs

  5. Hanford whole body counting manual

    Energy Technology Data Exchange (ETDEWEB)

    Palmer, H.E.; Brim, C.P.; Rieksts, G.A.; Rhoads, M.C.

    1987-05-01

    This document, a reprint of the Whole Body Counting Manual, was compiled to train personnel, document operation procedures, and outline quality assurance procedures. The current manual contains information on: the location, availability, and scope of services of Hanford's whole body counting facilities; the administrative aspect of the whole body counting operation; Hanford's whole body counting facilities; the step-by-step procedure involved in the different types of in vivo measurements; the detectors, preamplifiers and amplifiers, and spectroscopy equipment; the quality assurance aspect of equipment calibration and recordkeeping; data processing, record storage, results verification, report preparation, count summaries, and unit cost accounting; and the topics of minimum detectable amount and measurement accuracy and precision. 12 refs., 13 tabs.

  6. Post-Newtonian quasirigid body

    International Nuclear Information System (INIS)

    In this paper, we construct for the first time, in the first post-Newtonian (1PN) approximation, a complete model of a quasirigid body by means of a new constraint on the mass current density and mass density. In our 1PN quasirigid body model most of the relations, such as the spin vector proportional to the angular velocity, the definition of the moment of inertia tensor, the key relation between the mass quadrupole moment and the moment of inertia tensor, the rigid rotating formulas for the mass quadrupole moment, and the moment of inertia tensor, are just an extension of the main relations in the Newtonian rigid body model. When all of the 1/c2 terms are neglected, the 1PN quasirigid body model and the corresponding formulas reduce to the Newtonian version. A key relation is obtained in this paper for the first time, which might be very useful in future applications to problems in geodynamics and astronomy

  7. Suicide of a body packer.

    Science.gov (United States)

    Stichenwirth, M; Stelwag-Carion, C; Klupp, N; Hönigschnabl, S; Vycudilik, W; Bauer, G; Risser, D

    2000-01-24

    The smuggling of illicit drugs by means of body packing has become a common problem at European airports. Europe is considered to be the fastest growing market for cocaine worldwide, and the air route is the most frequently used method of trafficking cocaine. Smuggling illicit drugs by use of body packing is considered to be a high toxicological hazard because of the risk of leakage or a package bursting. We report about the first case of suicide of a body packer by re-ingesting the content of excreted cocaine packages. The consequence of this case is that the death scene investigation and autopsy assessments in case of a body packer's death should always consider the possibility of re-ingested packages. Detention personnel should be instructed by forensic and criminalistic experts to take preventive measures. PMID:10697780

  8. Aging changes in body shape

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/003998.htm Aging changes in body shape To use the sharing ... and both sexes. Height loss is related to aging changes in the bones, muscles, and joints. People ...

  9. JPL Small Body Database Browser

    Data.gov (United States)

    National Aeronautics and Space Administration — The JPL Small-Body Database Browser provides data for all known asteroids and many comets. Newly discovered objects and their orbits are added on a daily basis....

  10. Foreign body in the nose

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/000037.htm Foreign body in the nose To use the sharing ... JavaScript. This article discusses first aid for a foreign object placed into the nose. Considerations Curious young ...

  11. The three-body problem

    International Nuclear Information System (INIS)

    The three-body problem, which describes three masses interacting through Newtonian gravity without any restrictions imposed on the initial positions and velocities of these masses, has attracted the attention of many scientists for more than 300 years. In this paper, we present a review of the three-body problem in the context of both historical and modern developments. We describe the general and restricted (circular and elliptic) three-body problems, different analytical and numerical methods of finding solutions, methods for performing stability analysis and searching for periodic orbits and resonances. We apply the results to some interesting problems of celestial mechanics. We also provide a brief presentation of the general and restricted relativistic three-body problems, and discuss their astronomical applications. (review article)

  12. The Mind-Body Problem.

    Science.gov (United States)

    Fodor, Jerry A.

    1981-01-01

    Describes several different philosophies of mind with each philosophy's explanation of the mind-body problem. Philosophies discussed include dualism, materialism, functionalism, radical behaviorism, logical behaviorism and central-state identity. (DS)

  13. Body Image of Pakistani Consumers

    Directory of Open Access Journals (Sweden)

    Tariq Jalees

    2014-03-01

    Full Text Available This paper examines how media exposure, self-esteem, and religiosity influence body image of Pakistan consumers. The underpinning of this study is based on Social Comparison Theory with two additional variables (religiosity and self-esteem for understanding understand consumer attitude towards body image. A self-administered questionnaire was used. 193 persons responded at mall intercepts. The Conceptual framework was empirically tested through SEM. A positive relationship between exposure to media and body image was found while negative influence was found between self-esteem and body image. No relationship was found between religiosity and body image. The results showed mixed outcomes as compared to past studies. The scope of this study is limited to one city only and hence the finding could not be generalized. Future researcher may use a larger sample drawn throughout Pakistan, and by examining whether body image perception varies ethnically, age and gender. Media exposure is affecting the traditional norms and values of Pakistan culture and body image. The obsessions of the media and thin body image have to be controlled and this requires policy changes. Corporate sector might take initiative by not selecting ultra-thin models. They might also sponsors public service messages showing ill effects of being ultra-thin, and showing that the ultra-thin models are not real but is results of artworks and camera angles. Social Comparison Theory with two addition variable religiosity and self-esteem has been successfully extended/empirically tested in the domain of Pakistan culture.

  14. Foreign Body in Jugal Mucosa

    OpenAIRE

    Serrano, Thiago Luís Infanger; Pauna, Henrique Furlan; Hazboun, Igor Moreira; Dal Rio, Ana Cristina; Correa, Maria Elvira Pizzigatti; Nicola, Ester Maria Danielli

    2015-01-01

    Introduction Foreign body in the oral cavity may be asymptomatic for long time and only sometimes it can lead to a typical granulomatous foreign body reaction. Some patients may complain of oral pain and present signs of inflammation with purulent discharge. A granuloma is a distinct, compact microscopic structure composed of epithelioid-shaped macrophages typically surrounded by a rim of lymphocytes and filled with fibroblasts and collagen. Nowadays, the increase of cosmetic invasive procedu...

  15. Recognizing Body Dysmorphic Disorder (Dysmorphophobia)

    OpenAIRE

    Anukriti Varma; Rajesh Rastogi

    2015-01-01

    Dysmorphophobia is a psychiatric condition which frequently presents in the clinics of dermatologists and plastic surgeons. This disorder (also called body dysmorphic disorder) is troublesome to the patient whilst being confusing for the doctor. This commonly undiagnosed condition can be detected by a few simple steps. Timely referral to a psychiatrist benefits most patients suffering from it. This article describes with a case vignette, how to recognize body dysmorphic disorder presenting in...

  16. Body Image of Pakistani Consumers

    OpenAIRE

    Tariq Jalees; Ernest C de Run

    2014-01-01

    This paper examines how media exposure, self esteem, and religiosity influence body image of Pakistan consumers.The underpinning of this study is based on Social Comparison Theory with two additional variables (religiosity and self esteem) for understanding understand consumer attitude towards body image. A self administered questionnaire was used. 193 persons responded at mall intercepts. The Conceptual framework was empirically tested through SEM. A positive relationship between exposure to...

  17. Hatha yoga on body balance

    OpenAIRE

    Erick Tadeu Prado; Vagner Raso; Renata Coelho Scharlach; Cristiane Akemi Kasse

    2014-01-01

    Background: A good body balance requires a proper function of vestibular, visual, and somatosensory systems which can be reach with exercise practice and/or yoga. Aim: To determine the effects of a 5-month hatha yoga training program on body balance in young adults. Materials and Methods: This study used a controlled, nonrandomized design, where the experimental group underwent a 5-month training program and were then compared with the control group that had a sedentary lifestyle. A c...

  18. Dynamical Friction for Compound Bodies

    OpenAIRE

    Domínguez-Tenreiro, R.; Gómez-Flechoso, M. A.

    1997-01-01

    In the framework of the fluctuation-dissipation approach to dynamical friction, we derive an expression giving the orbital energy exchange experienced by a compound body as it moves interacting with a non homogeneous discrete background. The body is assumed to be composed of particles endowed with a velocity spectrum and with a non homogeneous spatial distribution. The Chandrasekhar formula is recovered in the limit of a point-like satellite with zero velocity dispersion and infinite temperat...

  19. Online Communication and Body Language

    OpenAIRE

    Alina Maria Hrisca

    2011-01-01

    bjectives: This article approaches the problem of body language, in the new context ofonline communication, trying to see how the latestdevelopment of technology influences it.PriorWork: The interest in body language has grown in the last decades, first because of the work ofscientists like Ekman, who studied micro-gestures and tried to give a universaldecoder,and secondbecause of the latest technological evolution in communication, that has stressed the importance ofnon-verbal cues.Approach:...

  20. What is a Human Body?

    DEFF Research Database (Denmark)

    Nissen, Ulrik Becker

    2016-01-01

    The essay offers an overview of different understandings of what a body is. As such, it can be read as an overview of what we mean, when we speak of a “human body”. However, the article also goes a step further; in the last section, a responsive understanding of the human body is outlined....... This is understood as responsiveness in three ways: viz an embodied self that responds to natural life, other human beings and, ultimately, to God....

  1. Railgun launch of small bodies

    Energy Technology Data Exchange (ETDEWEB)

    Drobyshevski, E.M.; Zhukov, B.G.; Sakharov, V.A. [Russian Academy of Sciences, St. Petersburg (Russian Federation). Ioffe Physico-Technical Inst.

    1995-01-01

    The small body launching using gas or plasma faces the fundamental problem caused by excess energy loss due to great wall surface/volume of the barrel ratio. That is why the efficiency of the plasma armature (PA) railgun acceleration is maximum for 8--10 mm-size bodies and drops as their size decreases. For the nuclear fusion applications, where {number_sign}1--2 mm-size pellets at 5--10 km/s velocity are desirable, one is forced to search for compromise between the body size (3--4 mm) and its velocity (3 km/s). Under these conditions, EM launchers did not demonstrate an advantage over the light-gas guns. When elaborating the {number_sign}1 mm railgun, the authors made use of the ideology of the body launching at constant acceleration close to the body strength or the electrode skin-layer explosion limits. That shortened the barrel length sufficiently. The system becomes highly compact thus permitting rapid test of new operation modes and different modifications of the design including the magnetic field augmentation. As a result of these refinements, the difficulties caused by the catastrophic supply of mass ablated from the electrodes were overcome and regimes of {number_sign}1 mm body non-sabot speed-up to 4.5 km/s were found. Potentialities of the small system created are far from being exhausted.

  2. Personality disorders and body weight.

    Science.gov (United States)

    Maclean, Johanna Catherine; Xu, Haiyong; French, Michael T; Ettner, Susan L

    2014-01-01

    We examine the impact of Axis II personality disorders (PDs) on body weight. PDs are psychiatric conditions that develop early in life from a mixture of genetics and environment, are persistent, and lead to substantial dysfunction for the affected individual. The defining characteristics of PDs conceptually link them with body weight, but the direction of the relationship likely varies across PD type. To investigate these links, we analyze data from Wave II of the National Epidemiological Survey of Alcohol and Related Conditions. We measure body weight with the body mass index (BMI) and a dichotomous indicator for obesity (BMI≥30). We find that women with PDs have significantly higher BMI and are more likely to be obese than otherwise similar women. We find few statistically significant or economically meaningful effects for men. Paranoid, schizotypal, and avoidant PDs demonstrate the strongest adverse impacts on women's body weight while dependent PD may be protective against elevated body weight among men. Findings from unconditional quantile regressions demonstrate a positive gradient between PDs and BMI in that the effects are greater for higher BMI respondents.

  3. Estimation of Body Weight from Body Size Measurements and Body Condition Scores in Dairy Cows

    DEFF Research Database (Denmark)

    Enevoldsen, Carsten; Kristensen, T.

    1997-01-01

    regimen. Results from this study indicate that a reliable model for estimating BW of very different dairy cows maintained in a wide range of environments can be developed using body condition score, demographic information, and measurements of hip height and hip width. However, for management purposes......The objective of this study was to evaluate the use of hip height and width, body condition score, and relevant demographic information to predict body weight (BW) of dairy cows. Seven regression models were developed from data from 972 observations of 554 cows. Parity, hip height, hip width......, substantial improvements can be obtained by developing models that are specific to a given site....

  4. [The anthropological approach to the "other body"].

    Science.gov (United States)

    Rozenberg, Jacques J

    2011-06-01

    Research into the anthropology of the body has tackled this wide-reaching concept of the "other body", highly relevant when applied to the field of nursing care. From the healthy biological body to the sick body, the history of anthropology shows the stages of acceptance and rejection of the "other body", male, female and animal.

  5. The body fades away: investigating the effects of transparency of an embodied virtual body on pain threshold and body ownership

    OpenAIRE

    Matteo Martini; Konstantina Kilteni; Antonella Maselli; Sanchez-Vives, Maria V.

    2015-01-01

    The feeling of “ownership” over an external dummy/virtual body (or body part) has been proven to have both physiological and behavioural consequences. For instance, the vision of an “embodied” dummy or virtual body can modulate pain perception. However, the impact of partial or total invisibility of the body on physiology and behaviour has been hardly explored since it presents obvious difficulties in the real world. In this study we explored how body transparency affects both body ownership ...

  6. Body composition: Where and when.

    Science.gov (United States)

    Mazzoccoli, Gianluigi

    2016-08-01

    The in vivo evaluation of body composition is essential in many clinical investigations, in order to accurately describe and monitor the nutritional status of a range of medical conditions and physiological processes, including sick and malnourished patients, pregnant women, breastfeeding women and the elderly, as well as in patients with cancer, osteoporosis and many other diseases. This research area is also important to the field of human nutrition and exercise physiology. Several research investigations have indicated the importance of measuring fat deposition in different body compartments, in order to gain a fuller understanding of the genetic factors that contribute to obesity, obesity-related disorders, such as dyslipidemia, and thereby to a fuller understanding of obesity associated cardio-metabolic disorders, with relevance to the relationship between body composition and energy expenditure. The spatial and temporal dimension, where and when, may influence the physiological relevance and the pathological implications of the fat composition of different body compartments, and, as such, is a new element to be considered when assessing body composition. PMID:26564096

  7. Hanford whole body counting manual

    Energy Technology Data Exchange (ETDEWEB)

    Palmer, H.E.; Rieksts, G.A.; Lynch, T.P.

    1990-06-01

    This document describes the Hanford Whole Body Counting Program as it is administered by Pacific Northwest Laboratory (PNL) in support of the US Department of Energy--Richland Operations Office (DOE-RL) and its Hanford contractors. Program services include providing in vivo measurements of internally deposited radioactivity in Hanford employees (or visitors). Specific chapters of this manual deal with the following subjects: program operational charter, authority, administration, and practices, including interpreting applicable DOE Orders, regulations, and guidance into criteria for in vivo measurement frequency, etc., for the plant-wide whole body counting services; state-of-the-art facilities and equipment used to provide the best in vivo measurement results possible for the approximately 11,000 measurements made annually; procedures for performing the various in vivo measurements at the Whole Body Counter (WBC) and related facilities including whole body counts; operation and maintenance of counting equipment, quality assurance provisions of the program, WBC data processing functions, statistical aspects of in vivo measurements, and whole body counting records and associated guidance documents. 16 refs., 48 figs., 22 tabs.

  8. Foreign bodies in the torax

    Directory of Open Access Journals (Sweden)

    Ćerimagić Zenaida

    2007-01-01

    Full Text Available Background. Metallic foreign bodies in the lung could be recognized using radiography. Non-metallic foreign bodies make difficulties because they are not as dense as metals. The aim of this report was to present the case of nonmetallic foreign bodies in the lung. Case report. A soldier of the Federation Army of Bosnia and Hertzegovina, injured in the explosion in 1998 was presented. The soldier was subjected to thoracic drainage and surgery tretment of the wound. After one year, the pulmologist treated him with tuberculostatics because of hemoptysis and pulmonary infiltration shown by a radiogram. This therapy had no effects, and the pulmologist presented this case to the thoracic surgeon who made thoracotomy removing a piece of wood (13 × 2 × 0.7 cm, 20 different-size pieces of wood, and a piece of textile from the lung. Conclusion. Patients with penetrating explosive lung injuries without metallic foreign bodies shown by a radiogram, with complications as hemoptisis, lung abscess, bronchiectasis, obstruction of the bronchus, chronic pneumonia should be subjected to thoracotomy for removing suspected foreign bodies without resecting the lung.

  9. Whole-body MRI screening

    Energy Technology Data Exchange (ETDEWEB)

    Puls, Ralf [HELIOS Klinikum Erfurt (Germany). Inst. of Diagnostic and Interventional Radiology and Neuroradiology; Hosten, Norbert (ed.) [Universitaetsklinikum Greifswald (Germany). Diagnostic Radiology and Neuroradiology

    2014-07-01

    The advent of dedicated whole-body MRI scanners has made it possible to image the human body from head to toe with excellent spatial resolution and with the sensitivity and specificity of conventional MR systems. A comprehensive screening examination by MRI relies on fast image acquisition, and this is now feasible owing to several very recent developments, including multichannel techniques, new surface coil systems, and automatic table movement. The daily analysis of whole-body MRI datasets uncovers many incidental findings, which are discussed by an interdisciplinary advisory board of physicians from all specialties. This book provides a systematic overview of these incidental findings with the aid of approximately 240 high-quality images. The radiologists involved in the project have written chapters on each organ system, presenting a structured compilation of the most common findings, their morphologic appearances on whole-body MRI, and guidance on their clinical management. Chapters on technical and ethical issues are also included. It is hoped that this book will assist other diagnosticians in deciding how to handle the most common incidental findings encountered when performing whole-body MRI.

  10. Whole-body MRI screening

    International Nuclear Information System (INIS)

    The advent of dedicated whole-body MRI scanners has made it possible to image the human body from head to toe with excellent spatial resolution and with the sensitivity and specificity of conventional MR systems. A comprehensive screening examination by MRI relies on fast image acquisition, and this is now feasible owing to several very recent developments, including multichannel techniques, new surface coil systems, and automatic table movement. The daily analysis of whole-body MRI datasets uncovers many incidental findings, which are discussed by an interdisciplinary advisory board of physicians from all specialties. This book provides a systematic overview of these incidental findings with the aid of approximately 240 high-quality images. The radiologists involved in the project have written chapters on each organ system, presenting a structured compilation of the most common findings, their morphologic appearances on whole-body MRI, and guidance on their clinical management. Chapters on technical and ethical issues are also included. It is hoped that this book will assist other diagnosticians in deciding how to handle the most common incidental findings encountered when performing whole-body MRI.

  11. Online Communication and Body Language

    Directory of Open Access Journals (Sweden)

    Alina Maria Hrisca

    2011-12-01

    Full Text Available bjectives: This article approaches the problem of body language, in the new context ofonline communication, trying to see how the latestdevelopment of technology influences it.PriorWork: The interest in body language has grown in the last decades, first because of the work ofscientists like Ekman, who studied micro-gestures and tried to give a universaldecoder,and secondbecause of the latest technological evolution in communication, that has stressed the importance ofnon-verbal cues.Approach: Using observation and the latest writing in the field, we will explain theconsequences that the use of avatars and online communication have on body language and itsinterpretation.Results:Excluding context, posture, micro-gestures, tone and so on, onlinecommunication does not only become stereotype, butalso affects real communication and especiallybody language. We can observe pragmatism of gestures, standardizations, lack of customizations,inability to read other’s body language etc.Implications:All of this shapes the Y-Generation, onethat not only fails to interpret other’s body language, but also is unable to express themselves in directcommunication.Value:This paper stresses out not only the consequencesof online communication,but also the importance of further technological development.

  12. Hanford whole body counting manual

    International Nuclear Information System (INIS)

    This document describes the Hanford Whole Body Counting Program as it is administered by Pacific Northwest Laboratory (PNL) in support of the US Department of Energy--Richland Operations Office (DOE-RL) and its Hanford contractors. Program services include providing in vivo measurements of internally deposited radioactivity in Hanford employees (or visitors). Specific chapters of this manual deal with the following subjects: program operational charter, authority, administration, and practices, including interpreting applicable DOE Orders, regulations, and guidance into criteria for in vivo measurement frequency, etc., for the plant-wide whole body counting services; state-of-the-art facilities and equipment used to provide the best in vivo measurement results possible for the approximately 11,000 measurements made annually; procedures for performing the various in vivo measurements at the Whole Body Counter (WBC) and related facilities including whole body counts; operation and maintenance of counting equipment, quality assurance provisions of the program, WBC data processing functions, statistical aspects of in vivo measurements, and whole body counting records and associated guidance documents. 16 refs., 48 figs., 22 tabs

  13. Genetics Home Reference: primary ciliary dyskinesia

    Science.gov (United States)

    ... tail-like structures, similar to cilia, that propel sperm cells forward. In the respiratory tract, cilia move ... of the flagella are necessary to propel the sperm cells forward to the female egg cell. Because ...

  14. Ciliary extracellular vesicles: Txt msg orgnlls

    OpenAIRE

    WANG, Juan; Barr, Maureen M.

    2016-01-01

    Cilia are sensory organelles that protrude from cell surfaces to monitor the surrounding environment. In addition to its role as sensory receiver, the cilium also releases extracellular vesicles (EVs). The release of sub-micron sized EVs is a conserved form of intercellular communication used by all three kingdoms of life. These extracellular organelles play important roles in both short and long range signaling between donor and target cells and may coordinate systemic responses within an or...

  15. How Is Primary Ciliary Dyskinesia Treated?

    Science.gov (United States)

    ... with your head down while you do CPT. Gravity and force help drain the mucus from your ... PCD, your doctor may prescribe antibiotics, bronchodilators, or anti-inflammatory medicines. These medicines help treat lung infections, ...

  16. On ciliary pumping and sieving in bryozoans

    DEFF Research Database (Denmark)

    Larsen, Poul Scheel; Riisgård, H. U.

    2002-01-01

    for the relation between velocity distribution in the lophophore and variation of pumping rate along tentacles. Typical variations are given and compared to those obtained by a more accurate two-dimensional numerical solution. Based on observed velocity distributions in the lophophore, particle tracks and tentacle...... flicks, a description is given of the feeding mechanisms. Particles entering the central region are brought to the mouth by the high velocity feeding current in the central part of the lophophore. Particles entering further out either escape between tentacles or are stopped by the laterofrontal cilia...... to be transferred to and conveyed by the frontal cilia bands on tentacles. The added load on laterofrontal cilia from viscous drag on a food particle retained by the cilia is found to be of the same order of magnitude or greater than the "background load" of viscous drag from fluid passing the laterofrontal cilia...

  17. A Unified Taxonomy for Ciliary Dyneins

    Science.gov (United States)

    Hom, Erik F.Y.; Witman, George B.; Harris, Elizabeth H.; Dutcher, Susan K.; Kamiya, Ritsu; Mitchell, David R.; Pazour, Gregory J.; Porter, Mary E.; Sale, Winfield S.; Wirschell, Maureen; Yagi, Toshiki; King, Stephen M.

    2011-01-01

    The formation and function of eukaryotic cilia/flagella require the action of a large array of dynein microtubule motor complexes. Due to genetic, biochemical, and microscopic tractability, Chlamydomonas reinhardtii has become the premier model system in which to dissect the role of dyneins in flagellar assembly, motility, and signaling. Currently, fifty-four proteins have been described as components of various Chlamydomonas flagellar dyneins or as factors required for their assembly in the cytoplasm and/or transport into the flagellum; orthologues of nearly all these components are present in other ciliated organisms including humans. For historical reasons, the nomenclature of these diverse dynein components and their corresponding genes, mutant alleles and orthologues has become extraordinarily confusing. Here, we unify Chlamydomonas dynein gene nomenclature and establish a systematic classification scheme based on structural properties of the encoded proteins. Furthermore, we provide detailed tabulations of the various mutant alleles and protein aliases that have been used and explicitly define the correspondence with orthologous components in other model organisms and humans. PMID:21953912

  18. -Mixed Intersection Bodies and Star Duality

    Indian Academy of Sciences (India)

    Zhao Chang-jian; Wing-Sum Cheung

    2010-09-01

    The paper extends the two notions of the dual mixed volumes and -intersection body to -dual mixed volumes and -mixed intersection body, respectively. Inequalities for the star dual of -mixed intersection bodies are established.

  19. Recognizing body dysmorphic disorder (dysmorphophobia

    Directory of Open Access Journals (Sweden)

    Anukriti Varma

    2015-01-01

    Full Text Available Dysmorphophobia is a psychiatric condition which frequently presents in the clinics of dermatologists and plastic surgeons. This disorder (also called body dysmorphic disorder is troublesome to the patient whilst being confusing for the doctor. This commonly undiagnosed condition can be detected by a few simple steps. Timely referral to a psychiatrist benefits most patients suffering from it. This article describes with a case vignette, how to recognize body dysmorphic disorder presenting in the dermatological or aesthetic surgery set up. Diagnostic criteria, eitiology, approach to patient, management strategy and when to refer are important learning points. The importance of recognizing this disorder timely and referring the patient to the psychiatrist for appropriate treatment is crucial.This article covers all aspects of body dysmorphic disorder relevant to dermatologists and plastic surgeons and hopes to be useful in a better understanding of this disorder.

  20. Recognizing Body Dysmorphic Disorder (Dysmorphophobia).

    Science.gov (United States)

    Varma, Anukriti; Rastogi, Rajesh

    2015-01-01

    Dysmorphophobia is a psychiatric condition which frequently presents in the clinics of dermatologists and plastic surgeons. This disorder (also called body dysmorphic disorder) is troublesome to the patient whilst being confusing for the doctor. This commonly undiagnosed condition can be detected by a few simple steps. Timely referral to a psychiatrist benefits most patients suffering from it. This article describes with a case vignette, how to recognize body dysmorphic disorder presenting in the dermatological or aesthetic surgery set up. Diagnostic criteria, eitiology, approach to patient, management strategy and when to refer are important learning points. The importance of recognizing this disorder timely and referring the patient to the psychiatrist for appropriate treatment is crucial. This article covers all aspects of body dysmorphic disorder relevant to dermatologists and plastic surgeons and hopes to be useful in a better understanding of this disorder. PMID:26644741

  1. Body piercing with fatal consequences.

    Science.gov (United States)

    Ranga, N; Jeffery, A J

    2011-01-01

    Body modifications such as piercings, tattoos and surgery have increased in popularity in recent times and have become more socially acceptable. The common complications of piercing different parts of the human anatomy are well-documented, including sepsis, allergic reactions and, more rarely, endocarditis and ischaemia. Deaths related to piercing complications are primarily septic in origin. In this case, a man in his 50s died due to complications of his multiple umbilical piercings. The cause of death was unusually linked to body modification; the umbilical piercings had ultimately led to a mesenteric infarction. Cases such as these are forensically important due to potential manslaughter charges that could be brought against a piercing establishment. More importantly, this case highlights another extreme complication of body modification. Fashion statements are always changing and impact upon many lives. It is important to highlight to people the potentially life-threatening complications of common piercing practices. PMID:22715261

  2. Seepage Anisotropy of Heterogeneous Body

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Ore dumps are heterogeneous bodies with anisotropic seepage characteristics because of the ore segregation.In an indoor experiment, a dump was constructed with three strata, where the horizontal and vertical seepage experiments were carried out.Horizontals flow are regarded as phreatic plan flows without penetration.Its seepage law satifies the Dupuit equation.With parallel lay seepage model, the equivalent seepage coefficient in the horizontal flow was obtained and was equivalent to the weighted mean of the seepage coefficient of each stratum.An unsaturated flow appeared in the vertical experiment, with a hydraulic gradient of 1.The vertical flow was equivalent to the seepage model that moved in vertical bedding; its equivalent seepage coefficient depended on the stratum with the minimum seepage coefficient.That the experiment showed clear anisotropy in a heterogeneous body was obvious with an anisotropic coefficient between 63 and 155, which is 25 to 100 times larger than that of a homogeneous body.

  3. Realistic Simulation for Body Area and Body-To-Body Networks.

    Science.gov (United States)

    Alam, Muhammad Mahtab; Ben Hamida, Elyes; Ben Arbia, Dhafer; Maman, Mickael; Mani, Francesco; Denis, Benoit; D'Errico, Raffaele

    2016-01-01

    In this paper, we present an accurate and realistic simulation for body area networks (BAN) and body-to-body networks (BBN) using deterministic and semi-deterministic approaches. First, in the semi-deterministic approach, a real-time measurement campaign is performed, which is further characterized through statistical analysis. It is able to generate link-correlated and time-varying realistic traces (i.e., with consistent mobility patterns) for on-body and body-to-body shadowing and fading, including body orientations and rotations, by means of stochastic channel models. The full deterministic approach is particularly targeted to enhance IEEE 802.15.6 proposed channel models by introducing space and time variations (i.e., dynamic distances) through biomechanical modeling. In addition, it helps to accurately model the radio link by identifying the link types and corresponding path loss factors for line of sight (LOS) and non-line of sight (NLOS). This approach is particularly important for links that vary over time due to mobility. It is also important to add that the communication and protocol stack, including the physical (PHY), medium access control (MAC) and networking models, is developed for BAN and BBN, and the IEEE 802.15.6 compliance standard is provided as a benchmark for future research works of the community. Finally, the two approaches are compared in terms of the successful packet delivery ratio, packet delay and energy efficiency. The results show that the semi-deterministic approach is the best option; however, for the diversity of the mobility patterns and scenarios applicable, biomechanical modeling and the deterministic approach are better choices. PMID:27104537

  4. Total body water and total body potassium in anorexia nervosa

    International Nuclear Information System (INIS)

    In the ill hospitalized patient with clinically relevant malnutrition, there is a measurable decrease in the ratio of the total body potassium to total body water (TBK/TBW) and a detectable increase in the ratio of total exchangeable sodium to total exchangeable potassium (Nae/Ke). To evaluate body composition analyses in anorexia nervosa patients with chronic uncomplicated semistarvation, TBK and TBW were measured by whole body K40 counting and deuterium oxide dilution in 10 females with stable anorexia nervosa and 10 age-matched female controls. The ratio of TBK/TBW was significantly (p less than 0.05) higher in anorexia nervosa patients than controls. The close inverse correlation found in published studies between TBK/TBW and Nae/Ke together with our results suggest that in anorexia nervosa, Nae/Ke may be low or normal. A decreased TBK/TBW is not a good indicator of malnutrition in the anorexia nervosa patient. The use of a decreased TBK/TBW ratio or an elevated Nae/Ke ratio as a definition of malnutrition may result in inappropriate nutritional management in the patient with severe nonstressed chronic semistarvation

  5. Cognitive-Behavioral Body Image Therapy for Body Dysmorphic Disorder.

    Science.gov (United States)

    Rosen, James C.; And Others

    1995-01-01

    Randomly assigned 54 body dysmorphic disorder (BDD) subjects to cognitive behavior therapy or no treatment. BDD symptoms were significantly decreased in therapy subjects and the disorder was eliminated in 82 percent of cases at posttreatment and 77 percent at follow-up. Subjects' overall psychological symptoms and self-esteem also improved. (RJM)

  6. Total body water and total body potassium in anorexia nervosa

    Energy Technology Data Exchange (ETDEWEB)

    Dempsey, D.T.; Crosby, L.O.; Lusk, E.; Oberlander, J.L.; Pertschuk, M.J.; Mullen, J.L.

    1984-08-01

    In the ill hospitalized patient with clinically relevant malnutrition, there is a measurable decrease in the ratio of the total body potassium to total body water (TBK/TBW) and a detectable increase in the ratio of total exchangeable sodium to total exchangeable potassium (Nae/Ke). To evaluate body composition analyses in anorexia nervosa patients with chronic uncomplicated semistarvation, TBK and TBW were measured by whole body K40 counting and deuterium oxide dilution in 10 females with stable anorexia nervosa and 10 age-matched female controls. The ratio of TBK/TBW was significantly (p less than 0.05) higher in anorexia nervosa patients than controls. The close inverse correlation found in published studies between TBK/TBW and Nae/Ke together with our results suggest that in anorexia nervosa, Nae/Ke may be low or normal. A decreased TBK/TBW is not a good indicator of malnutrition in the anorexia nervosa patient. The use of a decreased TBK/TBW ratio or an elevated Nae/Ke ratio as a definition of malnutrition may result in inappropriate nutritional management in the patient with severe nonstressed chronic semistarvation.

  7. Quantum three-body problems

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A scheme for dealing with the quantum three-body problem is presented to separate the rotational degrees of freedom completely from the internal ones. In this method, the three-body Schridinger equation is reduced to a system of coupled partial differential equations, depending only upon three internal variables. For arbitrary total orbital angular momentum l and the parity (-1)l+λ (λ=0 or 1), the number of the equations in this system is l+1-λ. By expanding the wavefunction with respect to a complete set of orthonormal basis functions, the system of equations is further reduced to a system of linear algebraic equations.

  8. Quantum three-body problems

    Institute of Scientific and Technical Information of China (English)

    马中骐

    2000-01-01

    A scheme for dealing with the quantum three-body problem is presented to separate the rotational degrees of freedom completely from the internal ones. In this method, the three-body Schrodinger equation is reduced to a system of coupled partial differential equations, depending only upon three internal variables. For arbitrary total orbital angular momentum l and the parity ( - 1)1+ λ (λ = 0 or 1), the number of the equations in this system is l + 1 - λ . By expanding the wavef unction with respect to a complete set of orthonormal basis functions, the system of equations is further reduced to a system of linear algebraic equations.

  9. Inclusion body myositis and myopathies.

    Science.gov (United States)

    Sivakumar, K; Dalakas, M C

    1997-10-01

    Sporadic inclusion body myositis is a frequent, acquired, adult-onset vacuolar myopathy affecting proximal and distal muscles with a distinct, easily identifiable clinical pattern. Although its primary cause is still unknown, autoimmune, viral, and degenerative processes, alone or in combination, are being considered. A uniform and sustained therapeutic response using the currently available immunomodulatory agents has not yet been achieved. Hereditary, inherited noninflammatory rimmed vacuolar myopathies with similar histologic features, collectively called hereditary inclusion body myopathies, are being redefined with the use of molecular genetics. The implications of the recent advances in clinical and basic sciences are discussed in the present review.

  10. Oxidative refolding from inclusion bodies.

    Science.gov (United States)

    Nelson, Christopher A; Lee, Chung A; Fremont, Daved H

    2014-01-01

    This protocol describes the growth and purification of bacterial inclusion body proteins with an option to selenomethionine label the targeted protein through feedback inhibition of methionine biosynthesis in common (non-auxotrophic) strains of E. coli. The method includes solubilization of inclusion body proteins by chemical denaturation and disulfide reduction, renaturation of the solubilized material through rapid dilution by pulsed injection into refolding buffer containing arginine and a mixture of oxidized and reduced glutathione, recovery of the recombinant protein using a stirred cell concentrator, and removal of the aggregated or misfolded fraction by passage over size-exclusion chromatography. The quality of the resulting protein can be assessed by SDS-PAGE.

  11. Body and learning inprofessional education

    DEFF Research Database (Denmark)

    Knudsen, Lars Emmerik Damgaard; Sillesen, Agnete

    and the development of the students own strategies and methods in their learning processes becomes present. The teachers own relationship to knowledge manifests in the corporeal incarnation of the situation by the way the teacher deals with the subject, which the students more or less consciously overtakes......Title: Body and learning in becoming professional Abstract: At University College South a development project between on body and learning video-recordings are used to examine the teacher’s appearance as a professional and his or her uses of bodily forms of expressions. The empirical data...

  12. Lexical-semantic body knowledge in 5- to 11-year-old children: How spatial body representation influences body semantics.

    Science.gov (United States)

    Auclair, Laurent; Jambaqué, Isabelle

    2015-01-01

    This study addresses the relation between lexico-semantic body knowledge (i.e., body semantics) and spatial body representation (i.e., structural body representation) by analyzing naming performances as a function of body structural topography. One hundred and forty-one children ranging from 5 years 2 months to 10 years 5 months old were asked to provide a lexical label for isolated body part pictures. We compared the children's naming performances according to the location of the body parts (body parts vs. head features and also upper vs. lower limbs) or to their involvement in motor skills (distal segments, joints, and broader body parts). The results showed that the children's naming performance was better for facial body parts than for other body parts. Furthermore, it was found that the naming of body parts was better for body parts related to action. These findings suggest that the development of a spatial body representation shapes the elaboration of semantic body representation processing. Moreover, this influence was not limited to younger children. In our discussion of these results, we focus on the important role of action in the development of body representations and semantic organization.

  13. Body segments and growth hormone.

    OpenAIRE

    Bundak, R; Hindmarsh, P. C.; Brook, C G

    1988-01-01

    The effects of human growth hormone treatment for five years on sitting height and subischial leg length of 35 prepubertal children with isolated growth hormone deficiency were investigated. Body segments reacted equally to treatment with human growth hormone; this is important when comparing the effect of growth hormone on the growth of children with skeletal dysplasias or after spinal irradiation.

  14. Calculating body frame size (image)

    Science.gov (United States)

    ... boned category. Determining frame size: To determine the body frame size, measure the wrist with a tape measure and use the following chart to determine whether the person is small, medium, or large boned. Women: Height under 5'2" Small = wrist size less ...

  15. Education and the Body: Prolegomena

    Science.gov (United States)

    Barrow, Robin

    2008-01-01

    There is a need to disentangle various distinct kinds of claim. The body may be important in schooling, though not in education. Movement, sport, fitness and health need to be distinguished. Does sport improve character? Does education imply knowledge about matters of health? Is learning to dance analogous to either learning to play cricket or…

  16. Body mass index in amputees.

    Science.gov (United States)

    Tzamaloukas, A H; Patron, A; Malhotra, D

    1994-01-01

    Whereas estimates of percent deviation of body weight from ideal (F delta weight) are corrected for amputation, those of body mass index (BMI) are not, creating discrepancies in evaluating obesity. A correction of the BMI formula for amputation is proposed. The formula for BMI was corrected for amputation mathematically. The mathematical model predicts that the uncorrected BMI formula underestimates body fat in unilateral amputees and overestimates body fat in subjects with bilateral amputations at the same length of the legs. F delta weight and corrected and uncorrected BMI estimates were computed in 15 subjects with unilateral leg amputation and in 8 subjects with multiple amputations. BMI estimates were as follows: in unilateral amputees, corrected 24.1 +/- 4.1 kg/m2, uncorrected 22.2 +/- 3.9 kg/m2 (p amputees, corrected 21.6 +/- 2.4 kg/m2, uncorrected 32.6 +/- 11.8 kg/m2 (p = .043). Linear regressions of F delta weight obtained from standard nutrition assessment on F delta weight computed from uncorrected and corrected BMI values were as follows: in unilateral amputees, uncorrected F delta weight = -0.079 + 0.932 x actual F delta weight, r = .974, p amputees, uncorrected F delta weight = 0.528 + 1.930 x actual F delta weight, r = .607, p is not significant, and corrected F delta weight = -0.010 + 0.920 x actual F delta weight, r = .936, p < .01.(ABSTRACT TRUNCATED AT 250 WORDS)

  17. Body dysmorphia and plastic surgery.

    Science.gov (United States)

    Kyle, Allison

    2012-01-01

    Body dysmorphic disorder is a mental disorder characterized by a preoccupation with some aspect of one's appearance. In cosmetic surgery, this preoccupation can be overlooked by practitioners resulting in a discrepancy between expected and realistic outcome. Identifying the characteristics of this disorder may be crucial to the practitioner-patient relationship in the plastic surgery setting. PMID:22929194

  18. Body proportions in Turner's syndrome.

    OpenAIRE

    Hughes, P C; Ribeiro, J; Hughes, I A

    1986-01-01

    Body proportion was studied in Turner's syndrome by measurement of standing an sitting heights in relation to chronological and bone age. The mean standard deviation score for standing height was -3.8. Disproportionate growth of the legs was not a major determinant of short stature, either before or after oestrogen replacement.

  19. Ovarian cancer and body size

    DEFF Research Database (Denmark)

    Mosgaard, Berit Jul

    2012-01-01

    Only about half the studies that have collected information on the relevance of women's height and body mass index to their risk of developing ovarian cancer have published their results, and findings are inconsistent. Here, we bring together the worldwide evidence, published and unpublished, and...

  20. Smart Body Sensor Object Networking

    Institute of Scientific and Technical Information of China (English)

    Bhumip Khasnabish

    2014-01-01

    This paper discusses smart body sensor objects (BSOs), including their networking and internetworking. Smartness can be incorpo-rated into BSOs by embedding virtualization, predictive analytics, and proactive computing and communications capabilities. A few use cases including the relevant privacy and protocol requirements are also presented. General usage and deployment eti-quette along with the relevant regulatory implications are then discussed.

  1. Sport, Modernity, and the Body

    NARCIS (Netherlands)

    N. Besnier; S. Brownell

    2012-01-01

    Over the past three decades, the important role that anthropological theory has bestowed on the body, modernity, nationalism, the state, citizenship, transnationalism, globalization, gender, and sexuality has placed sports at the center of questions central to the discipline. New approaches to the b

  2. Bodies Folded in Migrant Crypts

    DEFF Research Database (Denmark)

    Galis, Vasilis; Tzokas, Spyros; Tympas, Aristotle

    2016-01-01

    and human migrants generates a dis/abled subject. In this context, dis/ability may be a cause or consequence of migration, both in physical/material (the folding of bodies in the crypt) and cultural/semiotic terms, and may become a barrier to accessing protection, to entering and/or crossing a country...

  3. Variability in human body size

    Science.gov (United States)

    Annis, J. F.

    1978-01-01

    The range of variability found among homogeneous groups is described and illustrated. Those trends that show significantly marked differences between sexes and among a number of racial/ethnic groups are also presented. Causes of human-body size variability discussed include genetic endowment, aging, nutrition, protective garments, and occupation. The information is presented to aid design engineers of space flight hardware and equipment.

  4. A novel zf-MYND protein, CHB-3, mediates guanylyl cyclase localization to sensory cilia and controls body size of Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Manabi Fujiwara

    2010-11-01

    Full Text Available Cilia are important sensory organelles, which are thought to be essential regulators of numerous signaling pathways. In Caenorhabditis elegans, defects in sensory cilium formation result in a small-body phenotype, suggesting the role of sensory cilia in body size determination. Previous analyses suggest that lack of normal cilia causes the small-body phenotype through the activation of a signaling pathway which consists of the EGL-4 cGMP-dependent protein kinase and the GCY-12 receptor-type guanylyl cyclase. By genetic suppressor screening of the small-body phenotype of a cilium defective mutant, we identified a chb-3 gene. Genetic analyses placed chb-3 in the same pathway as egl-4 and gcy-12 and upstream of egl-4. chb-3 encodes a novel protein, with a zf-MYND motif and ankyrin repeats, that is highly conserved from worm to human. In chb-3 mutants, GCY-12 guanylyl cyclase visualized by tagged GFP (GCY-12::GFP fails to localize to sensory cilia properly and accumulates in cell bodies. Our analyses suggest that decreased GCY-12 levels in the cilia of chb-3 mutants may cause the suppression of the small-body phenotype of a cilium defective mutant. By observing the transport of GCY-12::GFP particles along the dendrites to the cilia in sensory neurons, we found that the velocities and the frequencies of the particle movement are decreased in chb-3 mutant animals. How membrane proteins are trafficked to cilia has been the focus of extensive studies in vertebrates and invertebrates, although only a few of the relevant proteins have been identified. Our study defines a new regulator, CHB-3, in the trafficking process and also shows the importance of ciliary targeting of the signaling molecule, GCY-12, in sensory-dependent body size regulation in C. elegans. Given that CHB-3 is highly conserved in mammal, a similar system may be used in the trafficking of signaling proteins to the cilia of other species.

  5. Between My Body and My "Dead Body": Narratives of Coma.

    Science.gov (United States)

    Meoded Danon, Limor

    2016-01-01

    This article is based on narrative research that focuses on corporeal experience during coma and during the rehabilitation process. Seventeen participants from different areas of Israel who had been in various kinds of coma states reveal what the corporeal experience of coma is. The participants are divided into three types of narrative protagonists--"dead-alive," "rational," and "emissaries." Each of the participants redefined the boundaries of the body, especially in cases when they spoke of experiences they did not understand as corporeal, for example, out-of-body experiences, near-death experiences, or experiences of being between the earthly and unearthly. Their struggle to find suitable words to tell their coma stories emphasizes these boundaries between experiencing and telling, which crossed the normative discursive border of the medical establishment and illustrates the ambiguous nature of human existence. PMID:25810464

  6. Reproduction in a carnivorous sponge: the significance of the absence of an aquiferous system to the sponge body plan.

    Science.gov (United States)

    Riesgo, Ana; Taylor, Chantel; Leys, Sally P

    2007-01-01

    Sponges usually produce, release, and capture gametes via the aquiferous system, and so the absence of both choanocytes and an aquiferous system in the carnivorous sponge Asbestopluma occidentalis has led to unusual characteristics of development for this Phylum. Sperm are highly specialized elongate cells tightly packed into spermatic cysts in the peripheral tissue of the sponge. Mature spermatozoa have proacrosomal vesicles at the anterior end and a ciliary pit surrounding the flagellum. Clusters of four to five oocytes are in synchronous stages of cleavage, suggesting that fertilization is synchronous. All stages of embryos occur in the same individual. Early cleavage was holoblastic and equal; blastomeres in two-, four- and eight-cell embryos were compact and 16-cell stage embryos were bi-layered. Late-stage embryos show three cellular regions along the anterior-posterior axis: the anterior hemisphere with heterogeneous cells, a mid-region with cells lying perpendicular to the A-P axis in a collagenous matrix, and small cells at the posterior pole. Unusually for Porifera, multiciliated cells cover all but the posterior pole. It is inferred that fertilization occurs by capture of intact spermatic cysts whose surrounding forceps spicules become trapped in the anisochelae of neighboring sponges. The elongate shape of sperm may be designed to penetrate the loose collagenous mesohyl, such that the arrival of a packet of sperm would lead to simultaneous fertilization of oocytes in a cluster. Loss of the water canal system in carnivorous sponges has allowed the evolution of features that are highly specialized for the habitat of this animal, but such modifications were not necessarily a prerequisite for the subsequent evolution of metazoans. Given the extremely versatile mechanisms of gametogenesis, embryogenesis, and tissue/body structure in sponges, generalizations regarding basal metazoan reproduction, development, and structure must be approached with caution.

  7. Small Bodies, Big Discoveries: NASA's Small Bodies Education Program

    Science.gov (United States)

    Mayo, L.; Erickson, K. J.

    2014-12-01

    2014 is turning out to be a watershed year for celestial events involving the solar system's unsung heroes, small bodies. This includes the close flyby of comet C/2013 A1 / Siding Spring with Mars in October and the historic Rosetta mission with its Philae lander to comet 67P/Churyumov-Gerasimenko. Beyond 2014, the much anticipated 2015 Pluto flyby by New Horizons and the February Dawn Mission arrival at Ceres will take center stage. To deliver the excitement and wonder of our solar system's small bodies to worldwide audiences, NASA's JPL and GSFC education teams in partnership with NASA EDGE will reach out to the public through multiple venues including broadcast media, social media, science and math focused educational activities, observing challenges, interactive visualization tools like "Eyes on the Solar System" and more. This talk will highlight NASA's focused education effort to engage the public in small bodies mission science and the role these objects play in our understanding of the formation and evolution of the solar system.

  8. Body image in non-western societies

    NARCIS (Netherlands)

    A. Edmonds

    2012-01-01

    This article discusses a range of body modification and conceptions of the body in non-Western societies. It also analyzes difficulties in applying the primarily Western psychological notion of body image to different societies. Body modification is a near human universal, but has many meanings and

  9. Body Image in the Dance Class

    Science.gov (United States)

    Oliver, Wendy

    2008-01-01

    Although some research has shown that dance enhances body image and self-esteem, other research shows that it sometimes has the opposite effect and causes dancers to develop a negative body image and even eating disorders. In dance, body image is not only about maintaining a certain weight; it can also refer to specific perceived body flaws.…

  10. Body water, extracellular water, body potassium, and exchangeable sodium in body builders using anabolic steroids

    International Nuclear Information System (INIS)

    Nine competitive male body builders aged 21 to 34 who were determined to take anabolic steroids were studied before and 6 to 10 weeks after a training cycle which included steroid administration. A control group of nine subjects matched in age and duration of competitive career, but using only natural training methods were studied on a single occasion while in training. Total body potassium (TBK) by 40K, total body water (TBW) by 3H2O dilution, extracellular water (ECW) by 35SO4 dilution and zero time extrapolation, and exchangeable sodium by 24Na dilution were measured before and after training. Intracellular water (ICW) was calculated from TBW - ECW. Initially steroid users had a greater skeletal muscle mass than control subjects, and obtained a further weight gain on steroids, all in skeletal muscle, based on parallel increases in TBK and ICW. Other body composition measurements did not change significantly. A single steroid user became ill taking steroids, decreased potassium by 5%, and increased extracellular water, changes which may represent the effects of hepatic dysfunction which occurred while on anabolic steroids

  11. Thermophoretic motion of bodies with axial symmetry

    International Nuclear Information System (INIS)

    Thermophoresis of axially symmetric bodies is investigated to first order in the Knudsen number, K n. The study is made in the limit where the typical length of the immersed body is small compared with the mean free path. It is shown that in this case, in contrast to what is the case for spherical bodies, the arising thermal force on the body is not in general anti-parallel to the temperature gradient. It is also shown that the gas exerts a torque on the body, which in magnitude and direction depends on the body geometry. Equations of motion describing the body movement are derived. Stationary solutions are studied

  12. Photography and body image in maturity

    Directory of Open Access Journals (Sweden)

    Helena Brandão Viana

    2014-03-01

    Full Text Available The Elder’s Body Image have become the object of several studies in recent years due to the influence that this has on the quality of life for theirselves. The objective of this research was to evaluate the perception of body image of elderly people through personal accounts and completion of a range of Body Image for Seniors. Through a photoshoot and application of a rating scale for body image levels of body satisfaction were evaluated.

  13. Body mass of late Quaternary mammals

    OpenAIRE

    Smith, F. A.; Lyons, S.K.; Ernest, S.K. Morgan; Jones, K E; Kaufman, D M; Dayan, T.; Marquet, P. A.; Haskell, J. P.

    2003-01-01

    The purpose of this data set was to compile body mass information for all mammals on Earth so that we could investigate the patterns of body mass seen across geographic and taxonomic space and evolutionary time. We were interested in the heritability of body size across taxonomic groups (How conserved is body mass within a genus, family, and order?), in the overall pattern of body mass across continents (Do the moments and other descriptive statistics remain the same across geographic space?)...

  14. Body parts reduction and self-objectification in the objectification of sexualized bodies

    OpenAIRE

    Bernard, Philippe; Gervais, Sarah; Allen, Jill; Campomizzi, Sophie; Klein, Olivier

    2015-01-01

    A recent study showed that people rely on piecemeal, analytic processing when viewing sexualized female (vs. male) bodies, suggesting that people perceived sexualized female bodies similarly to objects. This paper will examine whether cognitive objectification of sexualized female bodies is associated with a focus on women’s body parts rather than on their whole bodies. In order to test our assertion, we asked participants to view photographs of sexual body parts or entire bodies of males and...

  15. Rigid body dynamics of mechanisms

    CERN Document Server

    Hahn, Hubert

    2003-01-01

    The second volume of Rigid Body Dynamics of Mechanisms covers applications via a systematic method for deriving model equations of planar and spatial mechanisms. The necessary theoretical foundations have been laid in the first volume that introduces the theoretical mechanical aspects of mechatronic systems. Here the focus is on the application of the modeling methodology to various examples of rigid-body mechanisms, simple planar ones as well as more challenging spatial problems. A rich variety of joint models, active constraints, plus active and passive force elements is treated. The book is intended for self-study by working engineers and students concerned with the control of mechanical systems, i.e. robotics, mechatronics, vehicles, and machine tools. The examples included are a likely source from which to choose models for university lectures.

  16. The Body under the Mask

    DEFF Research Database (Denmark)

    Nørgård, Rikke Toft

    originating from "corporeal" philosophy and phenomenology (Leder, Merleau-Ponty, Sheets-Johnstone), as well as the holistic philosophy of martial arts (Funakoshi, Nakazawa), to unveil methodological possibilities that will enable an understanding of how gamers experience to a great extent is shaped...... cannot be understood as a simple question of ergonomics, motor skills or muscle memory - it isn't a passing phase as one "learns to play the game". However, my aim isn't to neglect the importance of "stereotypical" research, as if the gamer were "just a physical acting body". Rather, the goal...... is to enrich our notion of the gamer by giving him back his body, escaping the general trend towards the "decorporalized" gamer, and establishing a balanced holistic perspective....

  17. Treatment of intracranial foreign body.

    Science.gov (United States)

    Karadas, Sevdegul; Dursun, Recep; Kiymaz, Nejmi

    2014-07-01

    Craniospinal penetrating foreign body (FB) injuries are interesting, but rarely observed, cases. They are important in terms of the complications that they may cause. The etiologies of craniospinal penetrating injuries and intracranial FB are also different. Though a sewing needle is more rarely seen in an intracranial FB, it may occur as atttempted infanticide or as a result of an accident especially in early childhood before the closure of fontanels. We detected an intracranial sewing needle in the head radiograph of a case admitted to the emergency department for another reason. We present this case since this is a rare injury and the etiologies of craniospinal penetrating foreign body have different characteristics. PMID:25255596

  18. Esophagial Foreign Body in Children

    Directory of Open Access Journals (Sweden)

    Bayram Metin

    2014-04-01

    Full Text Available Esophageal foreign bodies (OYC are common in children and prevention and timely treatment is not a current problem that can cause serious morbidity and mortality. In the United States, depending on\\' OYC people a year die of approximately 1500-1600 . Gastrointestinal esophageal stricture at the narrowest point of destination, and contains in itself three. First stenosis at the level of the cricoid cartilage, the second stenosis, nearby the left main bronchus crosses the diaphragm and passes in the region and third place. This is due to the properties of 28-ie 68% esophageal foreign bodies are seen in the digestive tract. OYC anticipated or hidden for a long time the clinical diagnosis can be difficult and serious complications. OYC the removal of rigid esophagoscopy under general anesthesia is a safe and effective method. OYC the early diagnosis and treatment is very important for the prevention of life-threatening complications

  19. Quality assurance within regulatory bodies

    International Nuclear Information System (INIS)

    The IAEA directed extensive efforts during the years 1991 to 1995 to the integral revision of all NUSS quality assurance publications, which were approved and issued as Safety Series No.50-C/SG-Q, Quality Assurance for Safety in Nuclear Power Plants and other Nuclear Installations (1996). When these quality assurance publications were developed, their prime focus was on requirements against which work performed by the licensees could be measured and assessed by the regulatory bodies. In this way, they only helped to facilitate the functions of regulators. No requirements or recommendations were provided on how the regulators should ensure the effective implementation of their own activities. The present publication is a first attempt to collect, integrate and offer available experience to directly support performance of regulatory activities. It presents a comprehensive compilation on the application of quality assurance principles and methods by regulatory bodies to their activities. The aim is consistent good performance of regulatory activities through a systematic approach

  20. Spinning bodies in curved spacetime

    Science.gov (United States)

    d'Ambrosi, G.; Satish Kumar, S.; van de Vis, J.; van Holten, J. W.

    2016-02-01

    We study the motion of neutral and charged spinning bodies in curved spacetime in the test-particle limit. We construct equations of motion using a closed covariant Poisson-Dirac bracket formulation that allows for different choices of the Hamiltonian. We derive conditions for the existence of constants of motion and apply the formalism to the case of spherically symmetric spacetimes. We show that the periastron of a spinning body in a stable orbit in a Schwarzschild or Reissner-Nordstrøm background not only precesses but also varies radially. By analyzing the stability conditions for circular motion we find the innermost stable circular orbit (ISCO) as a function of spin. It turns out that there is an absolute lower limit on the ISCOs for increasing prograde spin. Finally we establish that the equations of motion can also be derived from the Einstein equations using an appropriate energy-momentum tensor for spinning particles.