WorldWideScience

Sample records for chs torsatron

  1. Configurational effects on low collision plasma confinement in CHS Heliotron/Torsatron

    Energy Technology Data Exchange (ETDEWEB)

    Heyn, M.F.; Kernbichler, W. [Institut fuer Theoretische Physik, Technische Universitaet Graz, Graz (Austria); Kasilov, S.V.; Nemov, V.V.; Pavlichenko, O.S. [Institute of Plasma Physics, NSC KIPT, Kharkov (Ukraine); Matsuoka, K.; Okamura, S. [National Inst. for Fusion Science, Toki, Gifu (Japan)

    2001-01-01

    Multihelicity effects on low collisionality ({approx}1/v) regime of neoclassical transport has been analyzed for full range of magnetic field configurations of CHS Heliotron/Torsatron. Transport coefficients for this regime has been calculated according to an approach developed in the previous paper. It was shown that the drift-orbit-optimized configuration of CHS device investigated in the previous report has the best confinement properties for low collision plasma confinement. (author)

  2. Assessment of torsatrons as reactors

    Energy Technology Data Exchange (ETDEWEB)

    Lyon, J.F. (Oak Ridge National Lab., TN (United States)); Painter, S.L. (Australian National Univ., Canberra, ACT (Australia))

    1992-12-01

    Stellarators have significant operational advantages over tokamaks as ignited steady-state reactors because stellarators have no dangerous disruptions and no need for continuous current drive or power recirculated to the plasma, both easing the first wall, blanket, and shield design; less severe constraints on the plasma parameters and profiles; and better access for maintenance. This study shows that a reactor based on the torsatron configuration (a stellarator variant) could also have up to double the mass utilization efficiency (MUE) and a significantly lower cost of electricity (COE) than a conventional tokamak reactor (ARIES-I) for a range of assumptions. Torsatron reactors can have much smaller coil systems than tokamak reactors because the coils are closer to the plasma and they have a smaller cross section (higher average current density because of the lower magnetic field). The reactor optimization approach and the costing and component models are those used in the current stage of the ARIES-I tokamak reactor study. Typical reactor parameters for a 1-GW(e) Compact Torsatron reactor example are major radius R[sub 0] = 6.6-8.8 m, on-axis magnetic field B[sup 0] = 4.8-7.5 T, B[sub max] (on coils) = 16 T, MUE 140-210 kW(e)/tonne, and COE (in constant 1990 dollars) = 67-79 mill/kW(e)h. The results are relatively sensitive to assumptions on the level of confinement improvement and the blanket thickness under the inboard half of the helical windings but relatively insensitive to other assumptions.

  3. Dicty_cDB: CHS403 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS403 (Link to dictyBase) - - - - CHS403P (Link to Original site) CHS403F 145 CHS403...Z 696 CHS403P 821 - - Show CHS403 Library CH (Link to library) Clone ID CHS403 (Link ...ukuba.ac.jp/CSM/CH/CHS4-A/CHS403Q.Seq.d/ Representative seq. ID CHS403P (Link to ...Original site) Representative DNA sequence >CHS403 (CHS403Q) /CSM/CH/CHS4-A/CHS403Q.Seq.d/ ATTAATATTAATAATTT...-cDNA Score E Sequences producing significant alignments: (bits) Value CHS403 (CHS403

  4. TOREX-4: a torsatron proof of principle experiment

    Energy Technology Data Exchange (ETDEWEB)

    Politzer, P A; Lidsky, L M; Montgomery, D B

    1979-03-01

    TOREX-4 is a torsatron Proof of Principle experiment designed to simultaneously achieve ntau approx. = to 5 x 10/sup 13/, n approx. = to 5 x 10/sup 14//cm/sup 3/, and T greater than or equal to 1 keV. TOREX-4 is capable of operating without externally driven currents; sufficient neutral beam power to reach betas of 2 to 5% is provided. The unique 4(+2) constant pitch angle winding configuration allows the reliable design of large systems with far greater experimental flexibility than can be achieved in conventional stellarators of comparable size. This will allow investigation of the basic physics questions of the torsatron configuration over a wide range of plasma properties and field configurations without sacrifice of the Proof of Principle goals. (MOW)

  5. Duplication and divergent evolution of the CHS and CHS-like genes in the chalcone synthase (CHS) superfamily

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The enzymes of the CHS-superfamily are responsible for biosynthesis of a wide range of natural products in plants. They are important for flower pigmentation, protection against UV light and defense against phytopathogens. Many plants were found to contain multiple copies of CHS genes. This review summarizes the recent progress in the studies of the CHS-superfamily, focusing on the duplication and divergent evolution of the CHS and CHS-like genes. Comparative analyses of gene structure, expression patterns and catalytic properties revealed extensive differentiation in both regulation and function among duplicate CHS genes. It is also proposed that the CHS-like enzymes in the CHS-superfamily evolved from CHS at different times in various organisms. The CHS-superfamily thus offers a valuable model to study the rates and patterns of sequence divergence between duplicate genes.

  6. Dicty_cDB: CHS470 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS470 (Link to dictyBase) - - - Contig-U16515-1 CHS470E (Link to Original site) CHS4...70F 567 CHS470Z 628 CHS470P 1175 CHS470E 1080 Show CHS470 Library CH (Link to library) Clone ID CHS4...ginal site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS470Q.Seq.d/ Re...presentative seq. ID CHS470E (Link to Original site) Representative DNA sequence >CHS470 (CHS470Q) /CSM/CH/CHS4-C/CHS4...rniilfff Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS470 (CHS470Q) /CSM/CH/CHS4-C/CHS4

  7. Dicty_cDB: CHS437 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS437 (Link to dictyBase) - - - Contig-U16244-1 CHS437E (Link to Original site) CHS4...37F 660 CHS437Z 390 CHS437P 1030 CHS437E 764 Show CHS437 Library CH (Link to library) Clone ID CHS4...inal site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS437Q.Seq.d/ Representative seq. ID CHS4...37E (Link to Original site) Representative DNA sequence >CHS437 (CHS437Q) /CSM/CH/CHS4-B/CHS4...J286 (SFJ286Q) /CSM/SF/SFJ2-D/SFJ286Q.Seq.d/ 1100 0.0 CHS437 (CHS437Q) /CSM/CH/CHS4-B/CHS4

  8. Dicty_cDB: CHS476 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS476 (Link to dictyBase) - - - Contig-U11855-1 - (Link to Original site) - - CHS4...76Z 711 - - - - Show CHS476 Library CH (Link to library) Clone ID CHS476 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS476Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS476 (CHS476Q) /CSM/CH/CHS4-D/CHS476Q.Seq.d/ XXXXXXXXXXTTTGATG...cDNA Score E Sequences producing significant alignments: (bits) Value CHS476 (CHS476Q) /CSM/CH/CHS4

  9. Dicty_cDB: CHS429 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS429 (Link to dictyBase) - - - Contig-U16419-1 CHS429P (Link to Original site) CHS4...29F 503 CHS429Z 835 CHS429P 1318 - - Show CHS429 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS429Q.Seq.d/ Representative seq. ID CHS4...29P (Link to Original site) Representative DNA sequence >CHS429 (CHS429Q) /CSM/CH/CHS4-B/CHS4...yvtp Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS429 (CHS429Q) /CSM/CH/CHS4-B/CHS4

  10. Dicty_cDB: CHS417 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS417 (Link to dictyBase) - - - Contig-U11332-1 CHS417P (Link to Original site) CHS4...17F 627 CHS417Z 711 CHS417P 1318 - - Show CHS417 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS417Q.Seq.d/ Representative seq. ID CHS4...17P (Link to Original site) Representative DNA sequence >CHS417 (CHS417Q) /CSM/CH/CHS4-A/CHS4...sslly Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS417 (CHS417Q) /CSM/CH/CHS4-A/CHS4

  11. Dicty_cDB: CHS430 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS430 (Link to dictyBase) - - - Contig-U16333-1 CHS430P (Link to Original site) CHS4...30F 354 CHS430Z 121 CHS430P 455 - - Show CHS430 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS430Q.Seq.d/ Representative seq. ID CHS4...30P (Link to Original site) Representative DNA sequence >CHS430 (CHS430Q) /CSM/CH/CHS4-B/CHS4...ing significant alignments: (bits) Value CHS430 (CHS430Q) /CSM/CH/CHS4-B/CHS430Q.Seq.d/ 391 e-108 SHJ588 (SH

  12. Dicty_cDB: CHS413 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS413 (Link to dictyBase) - - - Contig-U11551-1 CHS413P (Link to Original site) CHS4...13F 650 CHS413Z 686 CHS413P 1316 - - Show CHS413 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS413Q.Seq.d/ Representative seq. ID CHS4...13P (Link to Original site) Representative DNA sequence >CHS413 (CHS413Q) /CSM/CH/CHS4-A/CHS4...f Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS413 (CHS413Q) /CSM/CH/CHS4-A/CHS4

  13. Dicty_cDB: CHS419 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS419 (Link to dictyBase) - - - Contig-U15541-1 CHS419P (Link to Original site) CHS4...19F 603 CHS419Z 755 CHS419P 1338 - - Show CHS419 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS419Q.Seq.d/ Representative seq. ID CHS4...19P (Link to Original site) Representative DNA sequence >CHS419 (CHS419Q) /CSM/CH/CHS4-A/CHS4...nificant alignments: (bits) Value CHS419 (CHS419Q) /CSM/CH/CHS4-A/CHS419Q.Seq.d/ 2573 0.0 VHN621 (VHN621Q) /

  14. Dicty_cDB: CHS409 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS409 (Link to dictyBase) - - - Contig-U15639-1 CHS409P (Link to Original site) CHS4...09F 362 CHS409Z 718 CHS409P 1060 - - Show CHS409 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS409Q.Seq.d/ Representative seq. ID CHS4...09P (Link to Original site) Representative DNA sequence >CHS409 (CHS409Q) /CSM/CH/CHS4-A/CHS4...-cDNA Score E Sequences producing significant alignments: (bits) Value CHS409 (CHS409Q) /CSM/CH/CHS4-A/CHS40

  15. Dicty_cDB: CHS450 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS450 (Link to dictyBase) - - - Contig-U15648-1 CHS450P (Link to Original site) CHS4...50F 151 CHS450Z 571 CHS450P 702 - - Show CHS450 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS450Q.Seq.d/ Representative seq. ID CHS4...50P (Link to Original site) Representative DNA sequence >CHS450 (CHS450Q) /CSM/CH/CHS4-C/CHS4...nificant alignments: (bits) Value CHS450 (CHS450Q) /CSM/CH/CHS4-C/CHS450Q.Seq.d/ 1100 0.0 VFD470 (VFD470Q) /

  16. Dicty_cDB: CHS425 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS425 (Link to dictyBase) - - - Contig-U15541-1 CHS425P (Link to Original site) CHS4...25F 657 CHS425Z 756 CHS425P 1393 - - Show CHS425 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS425Q.Seq.d/ Representative seq. ID CHS4...25P (Link to Original site) Representative DNA sequence >CHS425 (CHS425Q) /CSM/CH/CHS4-B/CHS4...equences producing significant alignments: (bits) Value CHS425 (CHS425Q) /CSM/CH/CHS4-B/CHS4

  17. Dicty_cDB: CHS431 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS431 (Link to dictyBase) - - - Contig-U16430-1 CHS431P (Link to Original site) CHS4...31F 586 CHS431Z 751 CHS431P 1317 - - Show CHS431 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS431Q.Seq.d/ Representative seq. ID CHS4...31P (Link to Original site) Representative DNA sequence >CHS431 (CHS431Q) /CSM/CH/CHS4-B/CHS4...f*kkssn Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS431 (CHS431Q) /CSM/CH/CHS4-B/CHS4

  18. Dicty_cDB: CHS455 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS455 (Link to dictyBase) - - - Contig-U16525-1 CHS455P (Link to Original site) CHS4...55F 632 CHS455Z 601 CHS455P 1213 - - Show CHS455 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS455Q.Seq.d/ Representative seq. ID CHS4...55P (Link to Original site) Representative DNA sequence >CHS455 (CHS455Q) /CSM/CH/CHS4-C/CHS4...gnificant alignments: (bits) Value CHS455 (CHS455Q) /CSM/CH/CHS4-C/CHS455Q.Seq.d/

  19. Dicty_cDB: CHS434 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS434 (Link to dictyBase) - - - Contig-U11113-1 CHS434P (Link to Original site) CHS4...34F 691 CHS434Z 759 CHS434P 1430 - - Show CHS434 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS434Q.Seq.d/ Representative seq. ID CHS4...34P (Link to Original site) Representative DNA sequence >CHS434 (CHS434Q) /CSM/CH/CHS4-B/CHS4...re E Sequences producing significant alignments: (bits) Value CHS434 (CHS434Q) /CSM/CH/CHS4-B/CHS434Q.Seq.d/

  20. Dicty_cDB: CHS414 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS414 (Link to dictyBase) - - - Contig-U15707-1 CHS414P (Link to Original site) CHS4...14F 227 CHS414Z 474 CHS414P 681 - - Show CHS414 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS414Q.Seq.d/ Representative seq. ID CHS4...14P (Link to Original site) Representative DNA sequence >CHS414 (CHS414Q) /CSM/CH/CHS4-A/CHS4...) /CSM/VS/VSJ7-C/VSJ758Q.Seq.d/ 920 0.0 SHL585 (SHL585Q) /CSM/SH/SHL5-D/SHL585Q.Seq.d/ 920 0.0 CHS414 (CHS414Q) /CSM/CH/CHS4-A/CHS4

  1. Dicty_cDB: CHS443 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS443 (Link to dictyBase) - - - Contig-U11229-1 CHS443P (Link to Original site) CHS4...43F 614 CHS443Z 770 CHS443P 1364 - - Show CHS443 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS443Q.Seq.d/ Representative seq. ID CHS4...43P (Link to Original site) Representative DNA sequence >CHS443 (CHS443Q) /CSM/CH/CHS4-B/CHS4...apnlxflnk Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS443 (CHS443Q) /CSM/CH/CHS4-B/CHS4

  2. Dicty_cDB: CHS412 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS412 (Link to dictyBase) - - - Contig-U11295-1 CHS412P (Link to Original site) CHS4...12F 119 CHS412Z 498 CHS412P 597 - - Show CHS412 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS412Q.Seq.d/ Representative seq. ID CHS4...12P (Link to Original site) Representative DNA sequence >CHS412 (CHS412Q) /CSM/CH/CHS4-A/CHS4...logy vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS412 (CHS412Q) /CSM/CH/CHS4-A/CHS4

  3. Dicty_cDB: CHS466 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS466 (Link to dictyBase) - - - Contig-U15680-1 CHS466P (Link to Original site) CHS4...66F 619 CHS466Z 741 CHS466P 1340 - - Show CHS466 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS466Q.Seq.d/ Representative seq. ID CHS4...66P (Link to Original site) Representative DNA sequence >CHS466 (CHS466Q) /CSM/CH/CHS4-C/CHS4...g significant alignments: (bits) Value CHS466 (CHS466Q) /CSM/CH/CHS4-C/CHS466Q.Seq.d/ 1943 0.0 VHO863 (VHO86

  4. Dicty_cDB: CHS448 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS448 (Link to dictyBase) - - - Contig-U16426-1 CHS448P (Link to Original site) CHS4...48F 398 CHS448Z 672 CHS448P 1050 - - Show CHS448 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS448Q.Seq.d/ Representative seq. ID CHS4...48P (Link to Original site) Representative DNA sequence >CHS448 (CHS448Q) /CSM/CH/CHS4-B/CHS4...ces producing significant alignments: (bits) Value CHS448 (CHS448Q) /CSM/CH/CHS4-B/CHS448Q.Seq.d/ 2036 0.0 S

  5. Dicty_cDB: CHS477 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS477 (Link to dictyBase) - - - Contig-U16333-1 CHS477P (Link to Original site) CHS4...77F 579 CHS477Z 666 CHS477P 1225 - - Show CHS477 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS477Q.Seq.d/ Representative seq. ID CHS4...77P (Link to Original site) Representative DNA sequence >CHS477 (CHS477Q) /CSM/CH/CHS4-D/CHS4...re E Sequences producing significant alignments: (bits) Value CHS477 (CHS477Q) /CSM/CH/CHS4-D/CHS4

  6. Dicty_cDB: CHS486 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS486 (Link to dictyBase) - - - Contig-U15713-1 CHS486P (Link to Original site) CHS4...86F 586 CHS486Z 656 CHS486P 1222 - - Show CHS486 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS486Q.Seq.d/ Representative seq. ID CHS4...86P (Link to Original site) Representative DNA sequence >CHS486 (CHS486Q) /CSM/CH/CHS4-D/CHS4...equences producing significant alignments: (bits) Value CHS486 (CHS486Q) /CSM/CH/CHS4-D/CHS4

  7. Dicty_cDB: CHS484 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS484 (Link to dictyBase) - - - Contig-U10883-1 CHS484P (Link to Original site) CHS4...84F 628 CHS484Z 577 CHS484P 1185 - - Show CHS484 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS484Q.Seq.d/ Representative seq. ID CHS4...84P (Link to Original site) Representative DNA sequence >CHS484 (CHS484Q) /CSM/CH/CHS4-D/CHS4...s CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS484 (CHS484Q) /CSM/CH/CHS4-D/CHS4

  8. Dicty_cDB: CHS435 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS435 (Link to dictyBase) - - - Contig-U16471-1 CHS435P (Link to Original site) CHS4...35F 160 CHS435Z 800 CHS435P 940 - - Show CHS435 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS435Q.Seq.d/ Representative seq. ID CHS4...35P (Link to Original site) Representative DNA sequence >CHS435 (CHS435Q) /CSM/CH/CHS4-B/CHS4...Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS435 (CHS435Q) /CSM/CH/CHS4-B/CHS4

  9. Dicty_cDB: CHS461 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS461 (Link to dictyBase) - - - Contig-U15541-1 CHS461P (Link to Original site) CHS4...61F 642 CHS461Z 742 CHS461P 1364 - - Show CHS461 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS461Q.Seq.d/ Representative seq. ID CHS4...61P (Link to Original site) Representative DNA sequence >CHS461 (CHS461Q) /CSM/CH/CHS4-C/CHS4...Q FFQKNI Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS461 (CHS461Q) /CSM/CH/CHS4-C/CHS4

  10. Dicty_cDB: CHS107 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS107 (Link to dictyBase) - - - Contig-U11527-1 CHS107P (Link to Original site) CHS10...7F 486 CHS107Z 581 CHS107P 1047 - - Show CHS107 Library CH (Link to library) Clone ID CHS10...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS107Q.Seq.d/ Representative seq. ID CHS10...7P (Link to Original site) Representative DNA sequence >CHS107 (CHS107Q) /CSM/CH/CHS1-A/CHS10...oducing significant alignments: (bits) Value CHS107 (CHS107Q) /CSM/CH/CHS1-A/CHS107Q.Seq.d/ 1518 0.0 VHO413

  11. Dicty_cDB: CHS104 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS104 (Link to dictyBase) - - - - CHS104P (Link to Original site) CHS104F 633 CHS10...4Z 697 CHS104P 1310 - - Show CHS104 Library CH (Link to library) Clone ID CHS104 (Link...sukuba.ac.jp/CSM/CH/CHS1-A/CHS104Q.Seq.d/ Representative seq. ID CHS104P (Link to... Original site) Representative DNA sequence >CHS104 (CHS104Q) /CSM/CH/CHS1-A/CHS104Q.Seq.d/ TAGATGCTTCAAAATC...ducing significant alignments: (bits) Value CHS104 (CHS104Q) /CSM/CH/CHS1-A/CHS104Q.Seq.d/ 2478 0.0 SHL489 (

  12. Dicty_cDB: CHS105 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS105 (Link to dictyBase) - - - Contig-U16290-1 CHS105P (Link to Original site) CHS10...5F 570 CHS105Z 753 CHS105P 1303 - - Show CHS105 Library CH (Link to library) Clone ID CHS10...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS105Q.Seq.d/ Representative seq. ID CHS10...5P (Link to Original site) Representative DNA sequence >CHS105 (CHS105Q) /CSM/CH/CHS1-A/CHS10... E Sequences producing significant alignments: (bits) Value CHS105 (CHS105Q) /CSM/CH/CHS1-A/CHS105Q.Seq.d/ 1

  13. Dicty_cDB: CHS122 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS122 (Link to dictyBase) - - - Contig-U11458-1 CHS122P (Link to Original site) CHS1...22F 663 CHS122Z 819 CHS122P 1462 - - Show CHS122 Library CH (Link to library) Clone ID CHS1...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS122Q.Seq.d/ Representative seq. ID CHS1...22P (Link to Original site) Representative DNA sequence >CHS122 (CHS122Q) /CSM/CH/CHS1-A/CHS1...ant alignments: (bits) Value CHS122 (CHS122Q) /CSM/CH/CHS1-A/CHS122Q.Seq.d/ 2391 0.0 SSG440 (SSG440Q) /CSM/S

  14. Dicty_cDB: CHS124 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS124 (Link to dictyBase) - - - Contig-U16439-1 CHS124P (Link to Original site) CHS1...24F 587 CHS124Z 771 CHS124P 1338 - - Show CHS124 Library CH (Link to library) Clone ID CHS1...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS124Q.Seq.d/ Representative seq. ID CHS1...24P (Link to Original site) Representative DNA sequence >CHS124 (CHS124Q) /CSM/CH/CHS1-A/CHS1...nificant alignments: (bits) Value CHS124 (CHS124Q) /CSM/CH/CHS1-A/CHS124Q.Seq.d/ 2565 0.0 SHD220 (SHD220Q) /

  15. Dicty_cDB: CHS130 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS130 (Link to dictyBase) - - - Contig-U15941-1 CHS130P (Link to Original site) CHS1...30F 575 CHS130Z 798 CHS130P 1353 - - Show CHS130 Library CH (Link to library) Clone ID CHS1...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-B/CHS130Q.Seq.d/ Representative seq. ID CHS1...30P (Link to Original site) Representative DNA sequence >CHS130 (CHS130Q) /CSM/CH/CHS1-B/CHS1...DNA Score E Sequences producing significant alignments: (bits) Value CHS130 (CHS130Q) /CSM/CH/CHS1-B/CHS130Q

  16. Dicty_cDB: CHS140 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS140 (Link to dictyBase) - - - Contig-U16405-1 CHS140P (Link to Original site) CHS1...40F 553 CHS140Z 718 CHS140P 1251 - - Show CHS140 Library CH (Link to library) Clone ID CHS1...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-B/CHS140Q.Seq.d/ Representative seq. ID CHS1...40P (Link to Original site) Representative DNA sequence >CHS140 (CHS140Q) /CSM/CH/CHS1-B/CHS1...ucing significant alignments: (bits) Value CHS140 (CHS140Q) /CSM/CH/CHS1-B/CHS140Q.Seq.d/ 1392 0.0 AHK465 (A

  17. Dicty_cDB: CHS117 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS117 (Link to dictyBase) - - - Contig-U16471-1 CHS117P (Link to Original site) CHS1...17F 501 CHS117Z 771 CHS117P 1252 - - Show CHS117 Library CH (Link to library) Clone ID CHS1...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS117Q.Seq.d/ Representative seq. ID CHS1...17P (Link to Original site) Representative DNA sequence >CHS117 (CHS117Q) /CSM/CH/CHS1-A/CHS1...oducing significant alignments: (bits) Value CHS117 (CHS117Q) /CSM/CH/CHS1-A/CHS117Q.Seq.d/ 1487 0.0 SHJ637

  18. Dicty_cDB: CHS191 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS191 (Link to dictyBase) - - - Contig-U11946-1 CHS191P (Link to Original site) CHS19...1F 630 CHS191Z 689 CHS191P 1299 - - Show CHS191 Library CH (Link to library) Clone ID CHS19...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS191Q.Seq.d/ Representative seq. ID CHS19...1P (Link to Original site) Representative DNA sequence >CHS191 (CHS191Q) /CSM/CH/CHS1-D/CHS19...roducing significant alignments: (bits) Value CHS191 (CHS191Q) /CSM/CH/CHS1-D/CHS191Q.Seq.d/ 1130 0.0 VHK578

  19. Dicty_cDB: CHS421 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS421 (Link to dictyBase) - - - Contig-U16327-1 - (Link to Original site) - - CHS4...21Z 547 - - - - Show CHS421 Library CH (Link to library) Clone ID CHS421 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS421Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS421 (CHS421Q) /CSM/CH/CHS4-A/CHS421Q.Seq.d/ XXXXXXXXXXAAAAAAA...sl f Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS421 (CHS4

  20. Dicty_cDB: CHS441 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS441 (Link to dictyBase) - - - Contig-U16524-1 - (Link to Original site) CHS4...41F 174 - - - - - - Show CHS441 Library CH (Link to library) Clone ID CHS441 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS441Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS441 (CHS441Q) /CSM/CH/CHS4-B/CHS441Q.Seq.d/ CTGTAATAATATTAATA...s of secretory system 4.0 %: peroxisomal >> prediction for CHS441 is mit 5' end seq. ID CHS4

  1. Dicty_cDB: CHS174 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS174 (Link to dictyBase) - - - Contig-U16256-1 - (Link to Original site) CHS1...74F 593 - - - - - - Show CHS174 Library CH (Link to library) Clone ID CHS174 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS174Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS174 (CHS174Q) /CSM/CH/CHS1-D/CHS174Q.Seq.d/ CAAATCTTACAACTGTC...RSTIRIETTIELQ CPWYDFCGVCTGN--- Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS174 (CHS1

  2. Dicty_cDB: CHS150 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS150 (Link to dictyBase) - - - Contig-U11696-1 - (Link to Original site) CHS1...50F 168 - - - - - - Show CHS150 Library CH (Link to library) Clone ID CHS150 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-C/CHS150Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS150 (CHS150Q) /CSM/CH/CHS1-C/CHS150Q.Seq.d/ AAAAATTACTTTTAAAA...kkkkkkxgxxkkxgxx--- Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS194 (CHS1

  3. Dicty_cDB: CHS452 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS452 (Link to dictyBase) - - - Contig-U16313-1 CHS452P (Link to Original site) CHS4...52F 125 CHS452Z 455 CHS452P 560 - - Show CHS452 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS452Q.Seq.d/ Representative seq. ID CHS4...52P (Link to Original site) Representative DNA sequence >CHS452 (CHS452Q) /CSM/CH/CHS4-C/CHS4...kfhf**t*llw*iml* Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS452 (CHS452Q) /CSM/CH/CHS4

  4. Dicty_cDB: CHS482 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS482 (Link to dictyBase) - - - Contig-U13887-1 CHS482P (Link to Original site) CHS4...82F 676 CHS482Z 712 CHS482P 1368 - - Show CHS482 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS482Q.Seq.d/ Representative seq. ID CHS4...82P (Link to Original site) Representative DNA sequence >CHS482 (CHS482Q) /CSM/CH/CHS4-D/CHS4...dapaeepvatkxsk Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS482 (CHS482Q) /CSM/CH/CHS4

  5. Dicty_cDB: CHS436 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS436 (Link to dictyBase) - - - Contig-U14494-1 CHS436P (Link to Original site) CHS4...36F 133 CHS436Z 114 CHS436P 227 - - Show CHS436 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS436Q.Seq.d/ Representative seq. ID CHS4...36P (Link to Original site) Representative DNA sequence >CHS436 (CHS436Q) /CSM/CH/CHS4-B/CHS4...skeletal >> prediction for CHS436 is nuc 5' end seq. ID CHS436F 5' end seq. >CHS436F.Seq AAGCTGCTGCCGAACAAGC

  6. Dicty_cDB: CHS479 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS479 (Link to dictyBase) - - - Contig-U15935-1 CHS479P (Link to Original site) CHS4...79F 141 CHS479Z 733 CHS479P 854 - - Show CHS479 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS479Q.Seq.d/ Representative seq. ID CHS4...79P (Link to Original site) Representative DNA sequence >CHS479 (CHS479Q) /CSM/CH/CHS4-D/CHS4...qkxs*xdh *kn Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS479 (CHS479Q) /CSM/CH/CHS4

  7. Dicty_cDB: CHS472 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS472 (Link to dictyBase) - - - Contig-U16313-1 CHS472P (Link to Original site) CHS4...72F 617 CHS472Z 679 CHS472P 1276 - - Show CHS472 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS472Q.Seq.d/ Representative seq. ID CHS4...72P (Link to Original site) Representative DNA sequence >CHS472 (CHS472Q) /CSM/CH/CHS4-C/CHS4...ki***ikkqlphln Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS472 (CHS472Q) /CSM/CH/CHS4

  8. Dicty_cDB: CHS109 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS109 (Link to dictyBase) - - - Contig-U16471-1 CHS109P (Link to Original site) CHS10...9F 343 CHS109Z 728 CHS109P 1051 - - Show CHS109 Library CH (Link to library) Clone ID CHS10...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS109Q.Seq.d/ Representative seq. ID CHS10...9P (Link to Original site) Representative DNA sequence >CHS109 (CHS109Q) /CSM/CH/CHS1-A/CHS10...eticulum >> prediction for CHS109 is nuc 5' end seq. ID CHS109F 5' end seq. >CHS109F.Seq TTGATTTTATCATGTATCA

  9. Dicty_cDB: CHS440 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS440 (Link to dictyBase) - - - Contig-U15613-1 CHS440P (Link to Original site) CHS4...40F 139 CHS440Z 535 CHS440P 654 - - Show CHS440 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS440Q.Seq.d/ Representative seq. ID CHS4...40P (Link to Original site) Representative DNA sequence >CHS440 (CHS440Q) /CSM/CH/CHS4-B/CHS4...ACTFLTLIPVVLLANKIYKNL Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS440 (CHS4

  10. Dicty_cDB: CHS432 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS432 (Link to dictyBase) - - - Contig-U15902-1 CHS432P (Link to Original site) CHS4...32F 580 CHS432Z 768 CHS432P 1328 - - Show CHS432 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS432Q.Seq.d/ Representative seq. ID CHS4...32P (Link to Original site) Representative DNA sequence >CHS432 (CHS432Q) /CSM/CH/CHS4-B/CHS4...gymklffqtfmi*xyw ymkevxrnqfki*qk Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS432 (CHS4

  11. Dicty_cDB: CHS469 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS469 (Link to dictyBase) - - - Contig-U15708-1 CHS469P (Link to Original site) CHS4...69F 566 CHS469Z 732 CHS469P 1278 - - Show CHS469 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS469Q.Seq.d/ Representative seq. ID CHS4...69P (Link to Original site) Representative DNA sequence >CHS469 (CHS469Q) /CSM/CH/CHS4-C/CHS4...llxn*rf*klkyi*ikfyy iyk Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS469 (CHS4

  12. Dicty_cDB: CHS485 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS485 (Link to dictyBase) - - - Contig-U10823-1 CHS485P (Link to Original site) CHS4...85F 521 CHS485Z 704 CHS485P 1205 - - Show CHS485 Library CH (Link to library) Clone ID CHS4...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS485Q.Seq.d/ Representative seq. ID CHS4...85P (Link to Original site) Representative DNA sequence >CHS485 (CHS485Q) /CSM/CH/CHS4-D/CHS4...*w*pnqfi*ccf*fnslcfkfttaldesxkxfv Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS485 (CHS4

  13. Dicty_cDB: CHS405 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS405 (Link to dictyBase) - - - Contig-U11170-1 CHS405P (Link to Original site) CHS4...05F 138 CHS405Z 697 CHS405P 815 - - Show CHS405 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS405Q.Seq.d/ Representative seq. ID CHS4...05P (Link to Original site) Representative DNA sequence >CHS405 (CHS405Q) /CSM/CH/CHS4-A/CHS4...omology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS405 (CHS4

  14. Dicty_cDB: CHS439 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS439 (Link to dictyBase) - - - Contig-U16471-1 CHS439P (Link to Original site) CHS4...39F 117 CHS439Z 700 CHS439P 797 - - Show CHS439 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS439Q.Seq.d/ Representative seq. ID CHS4...39P (Link to Original site) Representative DNA sequence >CHS439 (CHS439Q) /CSM/CH/CHS4-B/CHS4...ear 32.0 %: cytoplasmic 12.0 %: mitochondrial 4.0 %: plasma membrane >> prediction for CHS439 is nuc 5' end seq. ID CHS4

  15. Dicty_cDB: CHS473 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS473 (Link to dictyBase) - - - Contig-U11696-1 CHS473P (Link to Original site) CHS4...73F 122 CHS473Z 131 CHS473P 233 - - Show CHS473 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS473Q.Seq.d/ Representative seq. ID CHS4...73P (Link to Original site) Representative DNA sequence >CHS473 (CHS473Q) /CSM/CH/CHS4-D/CHS4...vxxxxmvynshfniyltmvisav Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS473 (CHS4

  16. Dicty_cDB: CHS158 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS158 (Link to dictyBase) - - - Contig-U16313-1 CHS158P (Link to Original site) CHS1...58F 647 CHS158Z 746 CHS158P 1373 - - Show CHS158 Library CH (Link to library) Clone ID CHS1...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-C/CHS158Q.Seq.d/ Representative seq. ID CHS1...58P (Link to Original site) Representative DNA sequence >CHS158 (CHS158Q) /CSM/CH/CHS1-C/CHS1...n*fqfniti*tlsll*nliink kttapfk Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS158 (CHS1

  17. Dicty_cDB: CHS131 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS131 (Link to dictyBase) - - - Contig-U15897-1 - (Link to Original site) - - CHS1...31Z 765 - - - - Show CHS131 Library CH (Link to library) Clone ID CHS131 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-B/CHS131Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS131 (CHS131Q) /CSM/CH/CHS1-B/CHS131Q.Seq.d/ XXXXXXXXXXCAATCGA...VHA533Q) /CSM/VH/VHA5-B/VHA533Q.Seq.d/ 1491 0.0 CHS391 (CHS391Q) /CSM/CH/CHS3-D/CHS391Q.Seq.d/ 1491 0.0 CHS131 (CHS1

  18. Dicty_cDB: CHS134 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS134 (Link to dictyBase) - - - Contig-U15930-1 CHS134P (Link to Original site) CHS13...4F 649 CHS134Z 693 CHS134P 1322 - - Show CHS134 Library CH (Link to library) Clone ID CHS13...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-B/CHS134Q.Seq.d/ Representative seq. ID CHS13...4P (Link to Original site) Representative DNA sequence >CHS134 (CHS134Q) /CSM/CH/CHS1-B/CHS13...TIATQEGNTNNQ*in Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS134 (CHS13

  19. Dicty_cDB: CHS410 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS410 (Link to dictyBase) - - - Contig-U16471-1 - (Link to Original site) - - CHS4...10Z 705 - - - - Show CHS410 Library CH (Link to library) Clone ID CHS410 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS410Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS410 (CHS410Q) /CSM/CH/CHS4-A/CHS410Q.Seq.d/ XXXXXXXXXXGGTTCAC....0 %: cytoskeletal 4.0 %: mitochondrial 4.0 %: plasma membrane 4.0 %: endoplasmic reticulum >> prediction for CHS4

  20. Dicty_cDB: CHS487 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS487 (Link to dictyBase) - - - Contig-U16433-1 - (Link to Original site) - - CHS4...87Z 595 - - - - Show CHS487 Library CH (Link to library) Clone ID CHS487 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS487Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS487 (CHS487Q) /CSM/CH/CHS4-D/CHS487Q.Seq.d/ XXXXXXXXXXACTTTAG...EEITLDAIDNQDGTYTA AYSLVGNGRFSTGVKLN Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS4

  1. Dicty_cDB: CHS112 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS112 (Link to dictyBase) - - - Contig-U15579-1 - (Link to Original site) CHS1...12F 166 - - - - - - Show CHS112 Library CH (Link to library) Clone ID CHS112 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS112Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS112 (CHS112Q) /CSM/CH/CHS1-A/CHS112Q.Seq.d/ ATGAACATTTAATAAAT... mitochondrial 4.0 %: vacuolar 4.0 %: plasma membrane 4.0 %: extracellular, including cell wall >> prediction for CHS1

  2. Dicty_cDB: CHS137 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS137 (Link to dictyBase) - - - Contig-U16475-1 - (Link to Original site) - - CHS13...7Z 598 - - - - Show CHS137 Library CH (Link to library) Clone ID CHS137 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-B/CHS137Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS137 (CHS137Q) /CSM/CH/CHS1-B/CHS137Q.Seq.d/ XXXXXXXXXXATGTGTA... wall 4.0 %: cytoskeletal 4.0 %: plasma membrane >> prediction for CHS137 is cyt

  3. Dicty_cDB: CHS183 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available iol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS183Q.Seq.d/ Representative seq. ID - (Link to Original site) Representa...tive DNA sequence >CHS183 (CHS183Q) /CSM/CH/CHS1-D/CHS183Q.Seq.d/ AAAAAAATATAATTAAC

  4. Dicty_cDB: CHS388 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available iol.tsukuba.ac.jp/CSM/CH/CHS3-D/CHS388Q.Seq.d/ Representative seq. ID - (Link to Original site) Representa...tive DNA sequence >CHS388 (CHS388Q) /CSM/CH/CHS3-D/CHS388Q.Seq.d/ ATTTTATAAATATCATA

  5. Dicty_cDB: CHS462 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS462 (Link to dictyBase) - - - Contig-U16313-1 CHS462P (Link to Original site) CHS4...62F 124 CHS462Z 536 CHS462P 640 - - Show CHS462 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS462Q.Seq.d/ Representative seq. ID CHS4...62P (Link to Original site) Representative DNA sequence >CHS462 (CHS462Q) /CSM/CH/CHS4-C/CHS4...%: nuclear 12.0 %: cytoplasmic 8.0 %: plasma membrane 8.0 %: vesicles of secretory system 4.0 %: cytoskeletal >> prediction for CHS4

  6. Dicty_cDB: CHS427 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS427 (Link to dictyBase) - - - Contig-U16471-1 CHS427P (Link to Original site) CHS4...27F 121 CHS427Z 705 CHS427P 806 - - Show CHS427 Library CH (Link to library) Clone ID CHS4... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS427Q.Seq.d/ Representative seq. ID CHS4...27P (Link to Original site) Representative DNA sequence >CHS427 (CHS427Q) /CSM/CH/CHS4-B/CHS4...02Q) /CSM/SH/SHG5-A/SHG502Q.Seq.d/ 662 0.0 SHD178 (SHD178Q) /CSM/SH/SHD1-D/SHD178Q.Seq.d/ 662 0.0 CHS4

  7. Dicty_cDB: CHS192 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS192 (Link to dictyBase) - - - Contig-U16524-1 CHS192P (Link to Original site) CHS1...92F 681 CHS192Z 796 CHS192P 1457 - - Show CHS192 Library CH (Link to library) Clone ID CHS1...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS192Q.Seq.d/ Representative seq. ID CHS1...92P (Link to Original site) Representative DNA sequence >CHS192 (CHS192Q) /CSM/CH/CHS1-D/CHS1...igkekekqkqkeqi kenqrtnqqrkrrqketskxkxke Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS1

  8. Dicty_cDB: CHS132 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS132 (Link to dictyBase) - - - Contig-U16471-1 CHS132P (Link to Original site) CHS13...2F 121 CHS132Z 816 CHS132P 917 - - Show CHS132 Library CH (Link to library) Clone ID CHS13... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-B/CHS132Q.Seq.d/ Representative seq. ID CHS13...2P (Link to Original site) Representative DNA sequence >CHS132 (CHS132Q) /CSM/CH/CHS1-B/CHS13....00 60.0 %: nuclear 28.0 %: cytoplasmic 8.0 %: mitochondrial 4.0 %: plasma membrane >> prediction for CHS132

  9. Dicty_cDB: CHS480 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS480 (Link to dictyBase) - - - - - (Link to Original site) - - CHS4...80Z 557 - - - - Show CHS480 Library CH (Link to library) Clone ID CHS480 (Link to dictyBase) Atlas ID... - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS4...80Q.Seq.d/ Representative seq. ID - (Link to Original site) Representative DNA sequence >CHS4...80 (CHS480Q) /CSM/CH/CHS4-D/CHS480Q.Seq.d/ XXXXXXXXXXTACCAATGCTTTTGGTGCANCTAAAGGTGGTCGTG

  10. Dicty_cDB: CHS103 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS103 (Link to dictyBase) - - - - - (Link to Original site) - - CHS10...3Z 631 - - - - Show CHS103 Library CH (Link to library) Clone ID CHS103 (Link to dictyBase) Atlas ID... - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS10...3Q.Seq.d/ Representative seq. ID - (Link to Original site) Representative DNA sequence >CHS10...3 (CHS103Q) /CSM/CH/CHS1-A/CHS103Q.Seq.d/ XXXXXXXXXXGAAAGATTGTTATGCGATGAACAATACATTGTATT

  11. Dicty_cDB: CHS454 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS454 (Link to dictyBase) - - - Contig-U15591-1 | Contig-U15892-1 CHS4...54P (Link to Original site) CHS454F 629 CHS454Z 719 CHS454P 1328 - - Show CHS454 Library CH (Link to library) Clone ID CHS4...591-1 | Contig-U15892-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS4...54Q.Seq.d/ Representative seq. ID CHS454P (Link to Original site) Representative DNA sequence >CHS454 (CHS4...54Q) /CSM/CH/CHS4-C/CHS454Q.Seq.d/ CTTCCGGCACCAAAGAATGATATTGGAGGCTGGTGGATTTGGTTATATTATATATCACCC

  12. Dicty_cDB: CHS465 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS465 (Link to dictyBase) - - - Contig-U16313-1 - (Link to Original site) CHS4...65F 136 - - - - - - Show CHS465 Library CH (Link to library) Clone ID CHS465 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS465Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS465 (CHS465Q) /CSM/CH/CHS4-C/CHS465Q.Seq.d/ ACTGTTGGCCTACTGGT.../SH/SHB5-D/SHB591Q.Seq.d/ 82 2e-15 CHS465 (CHS465Q) /CSM/CH/CHS4-C/CHS465Q.Seq.d/ 82 2e-15 SHJ748 (SHJ748Q)

  13. Dicty_cDB: CHS493 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS493 (Link to dictyBase) - - - Contig-U13024-1 - (Link to Original site) - - CHS4...93Z 696 - - - - Show CHS493 Library CH (Link to library) Clone ID CHS493 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS493Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS493 (CHS493Q) /CSM/CH/CHS4-D/CHS493Q.Seq.d/ XXXXXXXXXXTTGATTT...ignificant alignments: (bits) Value CHS493 (CHS493Q) /CSM/CH/CHS4-D/CHS493Q.Seq.d

  14. Dicty_cDB: CHS433 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS433 (Link to dictyBase) - - - Contig-U15748-1 - (Link to Original site) CHS4...33F 675 - - - - - - Show CHS433 Library CH (Link to library) Clone ID CHS433 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS433Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS433 (CHS433Q) /CSM/CH/CHS4-B/CHS433Q.Seq.d/ TATTGGTATTATAAAAT...r*vit--- Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS433 (CHS433Q) /CSM/CH/CHS4-B/CHS4

  15. Dicty_cDB: CHS464 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS464 (Link to dictyBase) - - - Contig-U11316-1 - (Link to Original site) - - CHS4...64Z 586 - - - - Show CHS464 Library CH (Link to library) Clone ID CHS464 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS464Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS464 (CHS464Q) /CSM/CH/CHS4-C/CHS464Q.Seq.d/ XXXXXXXXXXTCCCACA...(SHG728Q) /CSM/SH/SHG7-B/SHG728Q.Seq.d/ 1142 0.0 CHS464 (CHS464Q) /CSM/CH/CHS4-C/CHS464Q.Seq.d/ 1142 0.0 AHF

  16. Dicty_cDB: CHS408 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS408 (Link to dictyBase) - - - - CHS408P (Link to Original site) CHS408F 644 CHS4...08Z 756 CHS408P 1380 - - Show CHS408 Library CH (Link to library) Clone ID CHS408 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4...-A/CHS408Q.Seq.d/ Representative seq. ID CHS408P (Link to... Original site) Representative DNA sequence >CHS408 (CHS408Q) /CSM/CH/CHS4-A/CHS408Q.Seq.d/ TCATCAGGCTCATCTA

  17. Dicty_cDB: CHS416 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS416 (Link to dictyBase) - - - Contig-U16419-1 - (Link to Original site) - - CHS4...16Z 855 - - - - Show CHS416 Library CH (Link to library) Clone ID CHS416 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS416Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS416 (CHS416Q) /CSM/CH/CHS4-A/CHS416Q.Seq.d/ XXXXXXXXXXCCACCAA...ignificant alignments: (bits) Value CHS416 (CHS416Q) /CSM/CH/CHS4-A/CHS416Q.Seq.d/ 1651 0.0 CHI869 (CHI869Q)

  18. Dicty_cDB: CHS453 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS453 (Link to dictyBase) - - - Contig-U16372-1 | Contig-U16442-1 CHS4...53P (Link to Original site) CHS453F 573 CHS453Z 602 CHS453P 1155 - - Show CHS453 Library CH (Link to library) Clone ID CHS4...372-1 | Contig-U16442-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS4...53Q.Seq.d/ Representative seq. ID CHS453P (Link to Original site) Representative DNA sequence >CHS453 (CHS4...53Q) /CSM/CH/CHS4-C/CHS453Q.Seq.d/ ATTTTCCCAAAATGAAACAAGATAAAATAAATGAAATTATAAATTATTTAAAATTTATTG

  19. Dicty_cDB: CHS418 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS418 (Link to dictyBase) - - - - CHS418P (Link to Original site) CHS418F 391 CHS4...18Z 720 CHS418P 1091 - - Show CHS418 Library CH (Link to library) Clone ID CHS418 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4...-A/CHS418Q.Seq.d/ Representative seq. ID CHS418P (Link to... Original site) Representative DNA sequence >CHS418 (CHS418Q) /CSM/CH/CHS4-A/CHS418Q.Seq.d/ AATTCAGTAGTTCAAT

  20. Dicty_cDB: CHS457 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS457 (Link to dictyBase) - - - Contig-U11403-1 - (Link to Original site) - - CHS4...57Z 741 - - - - Show CHS457 Library CH (Link to library) Clone ID CHS457 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS457Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS457 (CHS457Q) /CSM/CH/CHS4-C/CHS457Q.Seq.d/ XXXXXXXXXXTTTATGC...core E Sequences producing significant alignments: (bits) Value CHS457 (CHS457Q) /CSM/CH/CHS4-C/CHS457Q.Seq.

  1. Dicty_cDB: CHS415 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS415 (Link to dictyBase) - - - Contig-U15269-1 | Contig-U16515-1 CHS4...15P (Link to Original site) CHS415F 663 CHS415Z 494 CHS415P 1137 - - Show CHS415 Library CH (Link to library) Clone ID CHS4...269-1 | Contig-U16515-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS4...15Q.Seq.d/ Representative seq. ID CHS415P (Link to Original site) Representative DNA sequence >CHS415 (CHS4...15Q) /CSM/CH/CHS4-A/CHS415Q.Seq.d/ TATTGTTTAAATTTATAATGCTACAAGTTTTACAAAAATGAAAGTAATATATATTTATTT

  2. Dicty_cDB: CHS451 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS451 (Link to dictyBase) - - - Contig-U16411-1 | Contig-U16470-1 CHS4...51P (Link to Original site) CHS451F 549 CHS451Z 622 CHS451P 1151 - - Show CHS451 Library CH (Link to library) Clone ID CHS4...411-1 | Contig-U16470-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS4...51Q.Seq.d/ Representative seq. ID CHS451P (Link to Original site) Representative DNA sequence >CHS451 (CHS4...51Q) /CSM/CH/CHS4-C/CHS451Q.Seq.d/ AATAAATAATAATTAAAATTTGCATATATAAAAGAAATAAAAATTTTTTATTTTTTGTAA

  3. Dicty_cDB: CHS445 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS445 (Link to dictyBase) - - - Contig-U16411-1 | Contig-U16470-1 CHS4...45P (Link to Original site) CHS445F 533 CHS445Z 770 CHS445P 1283 - - Show CHS445 Library CH (Link to library) Clone ID CHS4...411-1 | Contig-U16470-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS4...45Q.Seq.d/ Representative seq. ID CHS445P (Link to Original site) Representative DNA sequence >CHS445 (CHS4...45Q) /CSM/CH/CHS4-B/CHS445Q.Seq.d/ ATTAATAAATAATAATTAAAATTTGCATATATAAAAGAAATAAAAATTTTTTATTTTTTG

  4. Dicty_cDB: CHS423 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS423 (Link to dictyBase) - - - Contig-U16471-1 - (Link to Original site) CHS4...23F 557 - - - - - - Show CHS423 Library CH (Link to library) Clone ID CHS423 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS423Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS423 (CHS423Q) /CSM/CH/CHS4-A/CHS423Q.Seq.d/ AAAAAATAATAAATTTA...eq.d/ 741 0.0 SHA112 (SHA112Q) /CSM/SH/SHA1-A/SHA112Q.Seq.d/ 741 0.0 CHS423 (CHS423Q) /CSM/CH/CHS4-A/CHS423Q

  5. Dicty_cDB: CHS459 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS459 (Link to dictyBase) - - - - CHS459P (Link to Original site) CHS459F 529 CHS4...59Z 762 CHS459P 1271 - - Show CHS459 Library CH (Link to library) Clone ID CHS459 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4...-C/CHS459Q.Seq.d/ Representative seq. ID CHS459P (Link to... Original site) Representative DNA sequence >CHS459 (CHS459Q) /CSM/CH/CHS4-C/CHS459Q.Seq.d/ ACTGTTGGCCTACTGG

  6. Dicty_cDB: CHS456 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS456 (Link to dictyBase) - - - - CHS456P (Link to Original site) CHS456F 607 CHS4...56Z 622 CHS456P 1209 - - Show CHS456 Library CH (Link to library) Clone ID CHS456 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4...-C/CHS456Q.Seq.d/ Representative seq. ID CHS456P (Link to... Original site) Representative DNA sequence >CHS456 (CHS456Q) /CSM/CH/CHS4-C/CHS456Q.Seq.d/ ATTATTGTTAAAATAA

  7. Dicty_cDB: CHS446 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS446 (Link to dictyBase) - - - Contig-U16475-1 | Contig-U16524-1 CHS4...46P (Link to Original site) CHS446F 193 CHS446Z 547 CHS446P 720 - - Show CHS446 Library CH (Link to library) Clone ID CHS4...75-1 | Contig-U16524-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS4...46Q.Seq.d/ Representative seq. ID CHS446P (Link to Original site) Representative DNA sequence >CHS446 (CHS4...46Q) /CSM/CH/CHS4-B/CHS446Q.Seq.d/ GTATGATTATTATAATTCAAATTAAAAATATATTATATAAATATATATAAATATATATTA

  8. Dicty_cDB: CHS468 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS468 (Link to dictyBase) - - - Contig-U16411-1 | Contig-U16470-1 CHS4...68P (Link to Original site) CHS468F 572 CHS468Z 776 CHS468P 1328 - - Show CHS468 Library CH (Link to library) Clone ID CHS4...411-1 | Contig-U16470-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS4...68Q.Seq.d/ Representative seq. ID CHS468P (Link to Original site) Representative DNA sequence >CHS468 (CHS4...68Q) /CSM/CH/CHS4-C/CHS468Q.Seq.d/ ACTGTTGGCCTACTGGATTAATAAATAATAATTAAAATTTGCATATATAAAAGAAATAAA

  9. Dicty_cDB: CHS467 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS467 (Link to dictyBase) - - - Contig-U16313-1 - (Link to Original site) - - CHS4...67Z 636 - - - - Show CHS467 Library CH (Link to library) Clone ID CHS467 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS467Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS467 (CHS467Q) /CSM/CH/CHS4-C/CHS467Q.Seq.d/ XXXXXXXXXXCATCAGA...CSM/SH/SHB4-A/SHB423Q.Seq.d/ 1241 0.0 SHA693 (SHA693Q) /CSM/SH/SHA6-D/SHA693Q.Seq.d/ 1241 0.0 CHS467 (CHS467Q) /CSM/CH/CHS4-C/CHS4

  10. Dicty_cDB: CHS401 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS401 (Link to dictyBase) - - - Contig-U10836-1 - (Link to Original site) CHS4...01F 630 - - - - - - Show CHS401 Library CH (Link to library) Clone ID CHS401 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS401Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS401 (CHS401Q) /CSM/CH/CHS4-A/CHS401Q.Seq.d/ ATCATATATTATATAAT...74Q) /CSM/SH/SHK5-D/SHK574Q.Seq.d/ 1027 0.0 CHS401 (CHS401Q) /CSM/CH/CHS4-A/CHS401Q.Seq.d/ 1027 0.0 CFH409 (

  11. Dicty_cDB: CHS471 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS471 (Link to dictyBase) - - - - CHS471P (Link to Original site) CHS471F 565 CHS4...71Z 695 CHS471P 1240 - - Show CHS471 Library CH (Link to library) Clone ID CHS471 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4...-C/CHS471Q.Seq.d/ Representative seq. ID CHS471P (Link to... Original site) Representative DNA sequence >CHS471 (CHS471Q) /CSM/CH/CHS4-C/CHS471Q.Seq.d/ ATTATTGGTGATACTA

  12. Dicty_cDB: CHS444 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS444 (Link to dictyBase) - - - - CHS444P (Link to Original site) CHS444F 402 CHS4...44Z 748 CHS444P 1130 - - Show CHS444 Library CH (Link to library) Clone ID CHS444 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4...-B/CHS444Q.Seq.d/ Representative seq. ID CHS444P (Link to... Original site) Representative DNA sequence >CHS444 (CHS444Q) /CSM/CH/CHS4-B/CHS444Q.Seq.d/ TATTAAAAAATTTTAA

  13. Dicty_cDB: CHS490 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS490 (Link to dictyBase) - - - Contig-U15689-1 | Contig-U16524-1 CHS4...90P (Link to Original site) CHS490F 164 CHS490Z 757 CHS490P 901 - - Show CHS490 Library CH (Link to library) Clone ID CHS4...89-1 | Contig-U16524-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS4...90Q.Seq.d/ Representative seq. ID CHS490P (Link to Original site) Representative DNA sequence >CHS490 (CHS4...90Q) /CSM/CH/CHS4-D/CHS490Q.Seq.d/ ATAATAATACAACAACAACAACAACATAATGTCAACACCTTTAAGAGGATCATCTCCTCA

  14. Dicty_cDB: CHS475 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS475 (Link to dictyBase) - - - Contig-U11507-1 | Contig-U16256-1 CHS4...75P (Link to Original site) CHS475F 583 CHS475Z 133 CHS475P 696 - - Show CHS475 Library CH (Link to library) Clone ID CHS4...07-1 | Contig-U16256-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS4...75Q.Seq.d/ Representative seq. ID CHS475P (Link to Original site) Representative DNA sequence >CHS475 (CHS4...75Q) /CSM/CH/CHS4-D/CHS475Q.Seq.d/ AATCATTTAAATAATAAAAATAATAATTAATAATAATAAAAATAATAATAACAAATTGAA

  15. Dicty_cDB: CHS481 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS481 (Link to dictyBase) - - - - CHS481P (Link to Original site) CHS481F 643 CHS4...81Z 735 CHS481P 1358 - - Show CHS481 Library CH (Link to library) Clone ID CHS481 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4...-D/CHS481Q.Seq.d/ Representative seq. ID CHS481P (Link to... Original site) Representative DNA sequence >CHS481 (CHS481Q) /CSM/CH/CHS4-D/CHS481Q.Seq.d/ AATTCAGTAGTTCAAT

  16. Dicty_cDB: CHS424 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS424 (Link to dictyBase) - - - Contig-U15033-1 - (Link to Original site) - - CHS4...24Z 620 - - - - Show CHS424 Library CH (Link to library) Clone ID CHS424 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS424Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS424 (CHS424Q) /CSM/CH/CHS4-A/CHS424Q.Seq.d/ XXXXXXXXXXTAATGAT...CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS424 (CHS424Q) /CSM/CH/CHS4-A/CHS4

  17. Dicty_cDB: CHS495 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS495 (Link to dictyBase) - - - Contig-U12401-1 | Contig-U16471-1 CHS4...95P (Link to Original site) CHS495F 516 CHS495Z 716 CHS495P 1212 - - Show CHS495 Library CH (Link to library) Clone ID CHS4...401-1 | Contig-U16471-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS4...95Q.Seq.d/ Representative seq. ID CHS495P (Link to Original site) Representative DNA sequence >CHS495 (CHS4...95Q) /CSM/CH/CHS4-D/CHS495Q.Seq.d/ ACAAAATGTTGAATTACCAACCTATGATCAAAATGAATNGGCAAAATTAATTCATAAAGT

  18. Dicty_cDB: CHS406 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS406 (Link to dictyBase) - G00285 DDB0233396 Contig-U16351-1 CHS4...06P (Link to Original site) CHS406F 433 CHS406Z 748 CHS406P 1161 - - Show CHS406 Library CH (Link to library) Clone ID CHS4...Contig-U16351-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS4...06Q.Seq.d/ Representative seq. ID CHS406P (Link to Original site) Representative DNA sequence >CHS406 (CHS406Q) /CSM/CH/CHS4...-A/CHS406Q.Seq.d/ AAAGTGTCGTACCACTATCACATATCATCTAATCTAGTGACTTTAAATAGTAGTAATTAT TTCTTTG

  19. Dicty_cDB: CHS404 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS404 (Link to dictyBase) - - - Contig-U16260-1 - (Link to Original site) - - CHS4...04Z 560 - - - - Show CHS404 Library CH (Link to library) Clone ID CHS404 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS404Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS404 (CHS404Q) /CSM/CH/CHS4-A/CHS404Q.Seq.d/ XXXXXXXXXXCACCAAA...ing significant alignments: (bits) Value CHS404 (CHS404Q) /CSM/CH/CHS4-A/CHS404Q.Seq.d/ 1072 0.0 VHO118 (VHO

  20. Dicty_cDB: CHS106 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS106 (Link to dictyBase) - - - Contig-U16381-1 | Contig-U16460-1 CHS10...6P (Link to Original site) CHS106F 487 CHS106Z 538 CHS106P 1005 - - Show CHS106 Library CH (Link to library) Clone ID CHS10...381-1 | Contig-U16460-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS10...6Q.Seq.d/ Representative seq. ID CHS106P (Link to Original site) Representative DNA sequence >CHS106 (CHS10...6Q) /CSM/CH/CHS1-A/CHS106Q.Seq.d/ ATGGTAATAGACAATGTGTTGAAGATCAAATTACATTGCCACCATTTGATAAATGTGATA

  1. Dicty_cDB: CHS102 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS102 (Link to dictyBase) - - - Contig-U16524-1 - (Link to Original site) CHS10...2F 201 - - - - - - Show CHS102 Library CH (Link to library) Clone ID CHS102 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS102Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS102 (CHS102Q) /CSM/CH/CHS1-A/CHS102Q.Seq.d/ TTGGCCTACTGGAAAAA...gy vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS102 (CHS102Q) /CSM/CH/CHS1-A/CHS10

  2. Dicty_cDB: CHS182 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS182 (Link to dictyBase) - - - Contig-U10924-1 | Contig-U12653-1 CHS1...82P (Link to Original site) CHS182F 610 CHS182Z 789 CHS182P 1379 - - Show CHS182 Library CH (Link to library) Clone ID CHS1...924-1 | Contig-U12653-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS1...82Q.Seq.d/ Representative seq. ID CHS182P (Link to Original site) Representative DNA sequence >CHS182 (CHS1...82Q) /CSM/CH/CHS1-D/CHS182Q.Seq.d/ ATTAAAAATTAATATAAATAAAATATAAAATAAAATGTCTACAGCAAAATTAGATTTTGT

  3. Dicty_cDB: CHS189 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS189 (Link to dictyBase) - - - - CHS189P (Link to Original site) CHS189F 339 CHS1...89Z 323 CHS189P 642 - - Show CHS189 Library CH (Link to library) Clone ID CHS189 (Link ...to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1...-D/CHS189Q.Seq.d/ Representative seq. ID CHS189P (Link to ...Original site) Representative DNA sequence >CHS189 (CHS189Q) /CSM/CH/CHS1-D/CHS189Q.Seq.d/ ATCAAATTATTTTTAAC

  4. Dicty_cDB: CHS133 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS133 (Link to dictyBase) - - - - CHS133P (Link to Original site) CHS133F 704 CHS1...33Z 773 CHS133P 1457 - - Show CHS133 Library CH (Link to library) Clone ID CHS133 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1...-B/CHS133Q.Seq.d/ Representative seq. ID CHS133P (Link to... Original site) Representative DNA sequence >CHS133 (CHS133Q) /CSM/CH/CHS1-B/CHS133Q.Seq.d/ ATTTTTAAATATATAT

  5. Dicty_cDB: CHS135 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS135 (Link to dictyBase) - - - Contig-U15348-1 | Contig-U16600-1 CHS1...35P (Link to Original site) CHS135F 583 CHS135Z 754 CHS135P 1317 - - Show CHS135 Library CH (Link to library) Clone ID CHS1...348-1 | Contig-U16600-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-B/CHS1...35Q.Seq.d/ Representative seq. ID CHS135P (Link to Original site) Representative DNA sequence >CHS135 (CHS1...35Q) /CSM/CH/CHS1-B/CHS135Q.Seq.d/ AAAAATAAATTAAATAACTTTTAAAACAAATAATTTAAATAAAAAAATAAAAAAATAAAA

  6. Dicty_cDB: CHS163 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS163 (Link to dictyBase) - - - Contig-U12984-1 | Contig-U16415-1 CHS1...63P (Link to Original site) CHS163F 470 CHS163Z 612 CHS163P 1062 - - Show CHS163 Library CH (Link to library) Clone ID CHS1...984-1 | Contig-U16415-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-C/CHS1...63Q.Seq.d/ Representative seq. ID CHS163P (Link to Original site) Representative DNA sequence >CHS163 (CHS1...63Q) /CSM/CH/CHS1-C/CHS163Q.Seq.d/ ATATTTACATATTTTTAAAAAATGGCAACAATTGAAGATACTTCAAAGATAATAACAATC

  7. Dicty_cDB: CHS194 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS194 (Link to dictyBase) - - - Contig-U11696-1 - (Link to Original site) CHS1...94F 159 - - - - - - Show CHS194 Library CH (Link to library) Clone ID CHS194 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS194Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS194 (CHS194Q) /CSM/CH/CHS1-D/CHS194Q.Seq.d/ ATAATTATAAAAATTAC...omology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS194 (CHS194Q) /CSM/CH/CHS1-D/CHS1

  8. Dicty_cDB: CHS178 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS178 (Link to dictyBase) - - - Contig-U15337-1 - (Link to Original site) - - CHS1...78Z 808 - - - - Show CHS178 Library CH (Link to library) Clone ID CHS178 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS178Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS178 (CHS178Q) /CSM/CH/CHS1-D/CHS178Q.Seq.d/ XXXXXXXXXXACTGGTA...Value SHA557 (SHA557Q) /CSM/SH/SHA5-C/SHA557Q.Seq.d/ 1582 0.0 CHS178 (CHS178Q) /CSM/CH/CHS1-D/CHS1

  9. Dicty_cDB: CHS169 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS169 (Link to dictyBase) - - - Contig-U16471-1 - (Link to Original site) - - CHS1...69Z 706 - - - - Show CHS169 Library CH (Link to library) Clone ID CHS169 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-C/CHS169Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS169 (CHS169Q) /CSM/CH/CHS1-C/CHS169Q.Seq.d/ XXXXXXXXXXGTTCACC...SH/SHD4-B/SHD429Q.Seq.d/ 666 0.0 CHS169 (CHS169Q) /CSM/CH/CHS1-C/CHS169Q.Seq.d/ 666 0.0 AHH414 (AHH414Q) /CS

  10. Dicty_cDB: CHS119 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS119 (Link to dictyBase) - - - Contig-U11262-1 - (Link to Original site) CHS1...19F 569 - - - - - - Show CHS119 Library CH (Link to library) Clone ID CHS119 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS119Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS119 (CHS119Q) /CSM/CH/CHS1-A/CHS119Q.Seq.d/ AATATTATTTATTTATT...e E Sequences producing significant alignments: (bits) Value CHS119 (CHS119Q) /CSM/CH/CHS1-A/CHS119Q.Seq.d/

  11. Dicty_cDB: CHS166 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS166 (Link to dictyBase) - - - - CHS166P (Link to Original site) CHS166F 433 CHS1...66Z 763 CHS166P 1176 - - Show CHS166 Library CH (Link to library) Clone ID CHS166 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1...-C/CHS166Q.Seq.d/ Representative seq. ID CHS166P (Link to... Original site) Representative DNA sequence >CHS166 (CHS166Q) /CSM/CH/CHS1-C/CHS166Q.Seq.d/ ATNATTATTGTTAAAA

  12. Dicty_cDB: CHS187 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS187 (Link to dictyBase) - G01715 DDB0185797 Contig-U11857-1 CHS1...87P (Link to Original site) CHS187F 627 CHS187Z 803 CHS187P 1410 - - Show CHS187 Library CH (Link to library) Clone ID CHS1...Contig-U11857-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS1...87Q.Seq.d/ Representative seq. ID CHS187P (Link to Original site) Representative DNA sequence >CHS187 (CHS187Q) /CSM/CH/CHS1...-D/CHS187Q.Seq.d/ AAATTCCAAATTATTTTAACACACTCACACACACACTGGAGAATTAAATAAAACGATAGA TAAAAAA

  13. Dicty_cDB: CHS190 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS190 (Link to dictyBase) - - - - CHS190P (Link to Original site) CHS190F 680 CHS1...90Z 762 CHS190P 1422 - - Show CHS190 Library CH (Link to library) Clone ID CHS190 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1...-D/CHS190Q.Seq.d/ Representative seq. ID CHS190P (Link to... Original site) Representative DNA sequence >CHS190 (CHS190Q) /CSM/CH/CHS1-D/CHS190Q.Seq.d/ CAATTAATATATTAAT

  14. Dicty_cDB: CHS142 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS142 (Link to dictyBase) - - - Contig-U16345-1 - (Link to Original site) CHS1...42F 601 - - - - - - Show CHS142 Library CH (Link to library) Clone ID CHS142 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-B/CHS142Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS142 (CHS142Q) /CSM/CH/CHS1-B/CHS142Q.Seq.d/ CTTGGAACTGGTCAATT...ant alignments: (bits) Value CHS142 (CHS142Q) /CSM/CH/CHS1-B/CHS142Q.Seq.d/ 1172 0.0 CHO845 (CHO845Q) /CSM/C

  15. Dicty_cDB: CHS186 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS186 (Link to dictyBase) - - - - CHS186P (Link to Original site) CHS186F 716 CHS1...86Z 770 CHS186P 1466 - - Show CHS186 Library CH (Link to library) Clone ID CHS186 (Link... to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1...-D/CHS186Q.Seq.d/ Representative seq. ID CHS186P (Link to... Original site) Representative DNA sequence >CHS186 (CHS186Q) /CSM/CH/CHS1-D/CHS186Q.Seq.d/ ATTGTATATAATATTT

  16. Dicty_cDB: CHS162 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS162 (Link to dictyBase) - - - Contig-U15935-1 - (Link to Original site) - - CHS1...62Z 745 - - - - Show CHS162 Library CH (Link to library) Clone ID CHS162 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-C/CHS162Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS162 (CHS162Q) /CSM/CH/CHS1-C/CHS162Q.Seq.d/ XXXXXXXXXXAACAGAT...CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS162 (CHS162Q) /CSM/CH/CHS1-C/CHS1

  17. Dicty_cDB: CHS111 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS111 (Link to dictyBase) - - - Contig-U11043-1 - (Link to Original site) - - CHS1...11Z 152 - - - - Show CHS111 Library CH (Link to library) Clone ID CHS111 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS111Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS111 (CHS111Q) /CSM/CH/CHS1-A/CHS111Q.Seq.d/ XXXXXXXXXXCCACCAC....Seq.d/ 90 1e-17 VHH658 (VHH658Q) /CSM/VH/VHH6-C/VHH658Q.Seq.d/ 90 1e-17 CHS111 (CHS111Q) /CSM/CH/CHS1-A/CHS1

  18. Dicty_cDB: CHS196 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS196 (Link to dictyBase) - - - Contig-U16411-1 | Contig-U16470-1 CHS19...6P (Link to Original site) CHS196F 587 CHS196Z 845 CHS196P 1412 - - Show CHS196 Library CH (Link to library) Clone ID CHS19...411-1 | Contig-U16470-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS19...6Q.Seq.d/ Representative seq. ID CHS196P (Link to Original site) Representative DNA sequence >CHS196 (CHS19...6Q) /CSM/CH/CHS1-D/CHS196Q.Seq.d/ CATAATAAATAATAATTAAAATTTGCATATATAAAAGAAATAAAAATTTTTTATTTTTTG

  19. Dicty_cDB: CHS193 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS193 (Link to dictyBase) - - - Contig-U16475-1 - (Link to Original site) - - CHS19...3Z 556 - - - - Show CHS193 Library CH (Link to library) Clone ID CHS193 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS193Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS193 (CHS193Q) /CSM/CH/CHS1-D/CHS193Q.Seq.d/ XXXXXXXXXXAAATGTG...icant alignments: (bits) Value SFE223 (SFE223Q) /CSM/SF/SFE2-A/SFE223Q.Seq.d/ 1082 0.0 CHS193 (CHS193Q) /CSM/CH/CHS1-D/CHS19

  20. Plasma engineering analysis of a small torsatron reactor

    Energy Technology Data Exchange (ETDEWEB)

    Lacatski, J.T.; Houlberg, W.A.; Uckan, N.A.

    1985-10-01

    This study examines the plasma physics and reactor engineering feasibility of a small, medium aspect ratio, high-beta, l = 2, D-T torsatron power reactor, based on the magnetic configuration of the Advanced Toroidal Facility, Oak Ridge National Laboratory. Plasma analyses are performed to assess whether confinement in a small, average radius plasma is sufficient to yield an ignited or high-Q driven device. Much of the physics assessment focuses on an evaluation of the radial electric field created by the nonambipolar particle flux. Detailed transport simulations are done with both fixed and self-consistent evolution of the radial electric field. Basic reactor engineering considerations taken into account are neutron wall loading, maximum magnetic field at the helical coils, coil shield thickness, and tritium breeding blanket-shield thickness.

  1. Dicty_cDB: CHS120 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS120 (Link to dictyBase) - - - Contig-U16524-1 - (Link to Original site) CHS1...20F 188 - - - - - - Show CHS120 Library CH (Link to library) Clone ID CHS120 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-A/CHS120Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS120 (CHS120Q) /CSM/CH/CHS1-A/CHS120Q.Seq.d/ AAAAAAATATTCAACTC... 4.0 %: Golgi >> prediction for CHS120 is nuc 5' end seq. ID CHS120F 5' end seq. >CHS120F.Seq AAAAAAATATTCAA

  2. Dicty_cDB: CHS138 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS138 (Link to dictyBase) - - - Contig-U11514-1 - (Link to Original site) - - CHS1...38Z 380 - - - - Show CHS138 Library CH (Link to library) Clone ID CHS138 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-B/CHS138Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS138 (CHS138Q) /CSM/CH/CHS1-B/CHS138Q.Seq.d/ XXXXXXXXXXGTATCAC...IPKEAT QVSHK Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS138 (CHS138Q) /CSM/CH/CHS1

  3. Dicty_cDB: CHS422 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS422 (Link to dictyBase) - - - Contig-U10806-1 - (Link to Original site) - - CHS4...22Z 759 - - - - Show CHS422 Library CH (Link to library) Clone ID CHS422 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS422Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS422 (CHS422Q) /CSM/CH/CHS4-A/CHS422Q.Seq.d/ XXXXXXXXXXGTGTTAA...lkilsqn* k*tlyifxkvi Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value CHS422 (CHS4

  4. Dicty_cDB: CHS161 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS161 (Link to dictyBase) - - - Contig-U16471-1 - (Link to Original site) - - CHS1...61Z 658 - - - - Show CHS161 Library CH (Link to library) Clone ID CHS161 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-C/CHS161Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS161 (CHS161Q) /CSM/CH/CHS1-C/CHS161Q.Seq.d/ XXXXXXXXXXATTGCTT.../SF/SFG2-A/SFG212Q.Seq.d/ 862 0.0 SFC806 (SFC806Q) /CSM/SF/SFC8-A/SFC806Q.Seq.d/ 862 0.0 CHS161 (CHS1

  5. Dicty_cDB: CHS151 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS151 (Link to dictyBase) - - - Contig-U12673-1 - (Link to Original site) - - CHS1...51Z 669 - - - - Show CHS151 Library CH (Link to library) Clone ID CHS151 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-C/CHS151Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS151 (CHS151Q) /CSM/CH/CHS1-C/CHS151Q.Seq.d/ XXXXXXXXXXTGGTTTC...ory system >> prediction for CHS151 is cyt 5' end seq. ID - 5' end seq. - Length of 5' end seq. - 3' end seq. ID CHS1

  6. Dicty_cDB: CHS181 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS181 (Link to dictyBase) - - - Contig-U16430-1 - (Link to Original site) - - CHS1...81Z 776 - - - - Show CHS181 Library CH (Link to library) Clone ID CHS181 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS181Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS181 (CHS181Q) /CSM/CH/CHS1-D/CHS181Q.Seq.d/ XXXXXXXXXXCATTCAT... %: Golgi >> prediction for CHS181 is nuc 5' end seq. ID - 5' end seq. - Length of 5' end seq. - 3' end seq. ID CHS1

  7. Dicty_cDB: CHS173 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS173 (Link to dictyBase) - - - Contig-U16313-1 - (Link to Original site) - - CHS1...73Z 721 - - - - Show CHS173 Library CH (Link to library) Clone ID CHS173 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS173Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS173 (CHS173Q) /CSM/CH/CHS1-D/CHS173Q.Seq.d/ XXXXXXXXXXGGTTTTA... 4.0 %: peroxisomal >> prediction for CHS173 is cyt 5' end seq. ID - 5' end seq. - Length of 5' end seq. - 3' end seq. ID CHS1

  8. Dicty_cDB: CHS411 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS411 (Link to dictyBase) - - - Contig-U16256-1 - (Link to Original site) - - CHS4...11Z 279 - - - - Show CHS411 Library CH (Link to library) Clone ID CHS411 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-A/CHS411Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS411 (CHS411Q) /CSM/CH/CHS4-A/CHS411Q.Seq.d/ XXXXXXXXXXAATGGTA...ic 4.0 %: vesicles of secretory system 4.0 %: extracellular, including cell wall 4.0 %: endoplasmic reticulum >> prediction for CHS4

  9. Dicty_cDB: CHS460 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS460 (Link to dictyBase) - - - Contig-U16471-1 - (Link to Original site) - - CHS4...60Z 796 - - - - Show CHS460 Library CH (Link to library) Clone ID CHS460 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-C/CHS460Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS460 (CHS460Q) /CSM/CH/CHS4-C/CHS460Q.Seq.d/ XXXXXXXXXXCCAGAGA...uclear 32.0 %: cytoplasmic 12.0 %: mitochondrial 4.0 %: cytoskeletal 4.0 %: plasma membrane >> prediction for CHS4

  10. Dicty_cDB: CHS447 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS447 (Link to dictyBase) - - - Contig-U16471-1 - (Link to Original site) - - CHS4...47Z 783 - - - - Show CHS447 Library CH (Link to library) Clone ID CHS447 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-B/CHS447Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS447 (CHS447Q) /CSM/CH/CHS4-B/CHS447Q.Seq.d/ XXXXXXXXXXTGAAGAA...rial 4.0 %: plasma membrane 4.0 %: endoplasmic reticulum >> prediction for CHS447 is nuc 5' end seq. ID - 5'

  11. Dicty_cDB: CHS153 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS153 (Link to dictyBase) - - - Contig-U16471-1 - (Link to Original site) - - CHS1...53Z 796 - - - - Show CHS153 Library CH (Link to library) Clone ID CHS153 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-C/CHS153Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS153 (CHS153Q) /CSM/CH/CHS1-C/CHS153Q.Seq.d/ XXXXXXXXXXCAGAGAG... 1.00 48.0 %: nuclear 32.0 %: cytoplasmic 12.0 %: mitochondrial 4.0 %: cytoskeletal 4.0 %: plasma membrane >> prediction for CHS1

  12. Dicty_cDB: CHS195 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS195 (Link to dictyBase) - - - Contig-U16423-1 - (Link to Original site) - - CHS19...5Z 671 - - - - Show CHS195 Library CH (Link to library) Clone ID CHS195 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS1-D/CHS195Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS195 (CHS195Q) /CSM/CH/CHS1-D/CHS195Q.Seq.d/ XXXXXXXXXXTTGAAAC....00 44.0 %: nuclear 28.0 %: cytoplasmic 20.0 %: cytoskeletal 4.0 %: mitochondrial 4.0 %: peroxisomal >> prediction for CHS19

  13. Virtual observatory publishing with DaCHS

    Science.gov (United States)

    Demleitner, M.; Neves, M. C.; Rothmaier, F.; Wambsganss, J.

    2014-11-01

    The Data Center Helper Suite DaCHS is an integrated publication package for building VO and Web services, supporting the entire workflow from ingestion to data mapping to service definition. It implements all major data discovery, data access, and registry protocols defined by the VO. DaCHS in this sense works as glue between data produced by the data providers and the standard protocols and formats defined by the VO. This paper discusses central elements of the design of the package and gives two case studies of how VO protocols are implemented using DaCHS' concepts.

  14. Virtual Observatory Publishing with DaCHS

    CERN Document Server

    Demleitner, Markus; Rothmaier, Florian; Wambsganss, Joachim

    2014-01-01

    The Data Center Helper Suite DaCHS is an integrated publication package for building Virtual Observatory (VO) and Web services, supporting the entire workflow from ingestion to data mapping to service definition. It implements all major data discovery, data access, and registry protocols defined by the VO. DaCHS in this sense works as glue between data produced by the data providers and the standard protocols and formats defined by the VO. This paper discusses central elements of the design of the package and gives two case studies of how VO protocols are implemented using DaCHS' concepts.

  15. Dicty_cDB: CHS494 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS494 (Link to dictyBase) - - - Contig-U16475-1 - (Link to Original site) - - CHS4...94Z 446 - - - - Show CHS494 Library CH (Link to library) Clone ID CHS494 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHS4-D/CHS494Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHS494 (CHS494Q) /CSM/CH/CHS4-D/CHS494Q.Seq.d/ XXXXXXXXXXGTCAATG...: Precursor; 96 5e-19 (Q54CH8) RecName: Full=Protein psiN; Flags: Precursor; 87 2e-16 S44208( S44208 ) extra

  16. Dicty_cDB: CHS203 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHS203 (Link to dictyBase) - - - Contig-U11332-1 - (Link to Or...iginal site) - - CHS203Z 727 - - - - Show CHS203 Library CH (Link to library) Clone ID CHS203 (Link to dicty...Base) Atlas ID - NBRP ID - dictyBase ID - Link to Contig Contig-U11332-1 Original site URL http://dictycdb.b...LSYDILSLLDS HLWLKKPLTISPQFLTMIGPELDPTLPNNPFKEFFNKLGTSSHDSGDNIDFKIPSFFEDN SRRHWGVETELSTGSCPICTLSLAENPESSFLGST...SYDILSLLDS HLWLKKPLTISPQFLTMIGPELDPTLPNNPFKEFFNKLGTSSHDSGDNIDFKIPSFFEDN SRRHWGVETELSTGSCPICTLSLAENPESSFLGSTP

  17. Stabilisation of ballooning modes in torsatrons with an externally applied toroidal current

    Energy Technology Data Exchange (ETDEWEB)

    Cooper, W.A. [Ecole Polytechnique Federale, Lausanne (Switzerland). Centre de Recherche en Physique des Plasma (CRPP)

    1996-09-01

    It has been found that ideal ballooning modes can impose very restrictive volume average {beta} limits in torsatrons much below the typical values close to 5% that are required to be economically realisable as reactor systems and it has been shown that externally applied toroidal currents that are peaked can destabilise the Mercier criterion in this type of configuration. We will show here that if the applied currents are hollow, they can stabilise the ballooning modes without triggering Mercier instabilities and as a result raise the limiting {beta}* from 2% to 5%. (author) 3 figs., 10 refs.

  18. Ripple transport in Helical-Axis Advanced Stellarators: A comparison with classical stellarator/torsatrons

    Energy Technology Data Exchange (ETDEWEB)

    Beidler, C.D. [Max-Planck-Institut fuer Plasmaphysik, Garching (Germany); Hitchon, W.N.G. [Wisconsin Univ., Madison, WI (United States). Dept. of Electrical and Computer Engineering

    1995-07-01

    Calculations of the neoclassical transport rates due to particles trapped in the helical ripples of a stellarator`s magnetic field are carried out, based on solutions of the bounceaveraged kinetic equation. These calculations employ a model for the magnetic field strength, B, which is an accurate approximation to the actual B for a wide variety of stellarator-type devices, among which are Helical-Axis Advanced Stellarators (Helias) as well as conventional stellarators and torsatrons. Comparisons are carried out in which it is shown that the Helias concept leads to significant reductions in neoclassical transport rates throughout the entire long-mean-free-path regime, with the reduction being particularly dramatic in the {nu}{sup {minus}1} regime. These findings are confirmed by numerical simulations. Further, it is shown that the behavior of deeply trapped particles in Helias can be fundamentally different from that in classical stellarator/torsatrons; as a consequence, the beneficial effects of a radial electric field on the transport make themselves felt at lower collision frequency than is usual.

  19. Spectroscopic Classification of PS16chs with SOAR/Goodman

    Science.gov (United States)

    Miller, J. A.; Hounsell, R. A.; Pan, Y.-C.; Foley, R. J.; Jha, S. W.; Rest, A.; Scolnic, D.

    2016-05-01

    We report the classification of PS16chs from spectroscopic observation with the Goodman spectrograph on the SOAR telescope. The observation was made on 2016 May 08 UT. We classify PS16chs as a SN Ia near maximum light at z = 0.19.

  20. Proto-CIRCUS tilted-coil tokamak–torsatron hybrid: Design and construction

    Energy Technology Data Exchange (ETDEWEB)

    Clark, A.W.; Doumet, M.; Hammond, K.C. [Department of Applied Physics and Applied Mathematics, Columbia University, New York, NY 10027 (United States); Kornbluth, Y. [Yeshiva University, New York, NY 10033 (United States); Spong, D.A. [Oak Ridge National Laboratory, Oak Ridge, TN 37830 (United States); Sweeney, R. [Department of Applied Physics and Applied Mathematics, Columbia University, New York, NY 10027 (United States); Volpe, F.A., E-mail: fvolpe@columbia.edu [Department of Applied Physics and Applied Mathematics, Columbia University, New York, NY 10027 (United States)

    2014-11-15

    Highlights: • A tokamak-like device with tilted toroidal field (TF) coils needs less plasma current than a conventional tokamak. • Rotational transform is partly generated by external coils. Device can be considered a tokamak–torsatron hybrid. • We designed and constructed the first device of this type. • Tilted TF coils are interlinked to each other, which helps to reduce aspect ratio of plasma. • This is a six-coil generalization of CNT stellarator, also at Columbia University, which features two interlinked coils. - Abstract: We present the field-line modeling, design, and construction of a prototype circular-coil tokamak–torsatron hybrid called Proto-CIRCUS. The device has a major radius R = 16 cm and minor radius a < 5 cm. The six “toroidal field” coils are planar as in a tokamak, but they are tilted. This, combined with induced or driven plasma current, is expected to generate rotational transform, as seen in field-line tracing and equilibrium calculations. The device is expected to operate at lower plasma current than a tokamak of comparable size and magnetic field, which might have interesting implications for disruptions and steady-state operation. Additionally, the toroidal magnetic ripple is less pronounced than in an equivalent tokamak in which the coils are not tilted. The tilted coils are interlocked, resulting in a relatively low aspect ratio, and can be moved, both radially and in tilt angle, between discharges. This capability will be exploited for detailed comparisons between calculations and field-line mapping measurements. Such comparisons will reveal whether this relatively simple concept can generate the expected rotational transform.

  1. Proto-CIRCUS Tilted-Coil Tokamak-Torsatron Hybrid: Design and Construction

    CERN Document Server

    Clark, A W; Hammond, K C; Kornbluth, Y; Spong, D A; Sweeney, R; Volpe, F A

    2014-01-01

    We present the field-line modeling, design and construction of a prototype circular-coil tokamak-torsatron hybrid called Proto-CIRCUS. The device has a major radius R = 16 cm and minor radius a < 5 cm. The six "toroidal field" coils are planar as in a tokamak, but they are tilted. This, combined with induced or driven plasma current, is expected to generate rotational transform, as seen in field-line tracing and equilibrium calculations. The device is expected to operate at lower plasma current than a tokamak of comparable size and magnetic field, which might have interesting implications for disruptions and steady-state operation. Additionally, the toroidal magnetic ripple is less pronounced than in an equivalent tokamak in which the coils are not tilted. The tilted coils are interlocked, resulting in a relatively low aspect ratio, and can be moved, both radially and in tilt angle, between discharges. This capability will be exploited for detailed comparisons between calculations and field-line mapping me...

  2. Characteristics of the three-half-turn-antenna-driven RF discharge in the Uragan-3M torsatron

    Energy Technology Data Exchange (ETDEWEB)

    Grigor’eva, L. I.; Chechkin, V. V., E-mail: chechkin@ipp.kharkov.ua; Moiseenko, V. E.; Grekov, D. L.; Pavlichenko, R. O.; Lozin, A. V.; Tarasov, I. K.; Kulaga, A. Ye.; Zamanov, N. V.; Tretiak, K. K.; Kozulya, M. M.; Beletskii, A. A.; Kasilov, A. A.; Mironov, Yu. K.; Romanov, V. S.; Voitsenya, V. S. [National Science Center Kharkiv Institute of Physics and Technology, Institute of Plasma Physics (Ukraine)

    2015-12-15

    In the ℓ = 3 Uragan-3M torsatron hydrogen plasma is produced by RF fields in the Alfvén range of frequencies (ω ≤ ω{sub ci}). The initial (target) plasma with the line-averaged density of units 10{sup 12} cm{sup −3} is produced by a frame antenna with a broad spectrum of generated parallel wavenumbers. After this, to heat the plasma and bring its density to ∼10{sup 13} cm{sup –3}, another, shorter wavelength three-half-turn antenna with large transverse currents is used. The behavior of the density, electron temperature, and loss of the plasma supported by the three-half-turn antenna is studied depending on the RF power fed to the antenna and initial values of the density and electron temperature supplied by the frame antenna.

  3. A missense mutation in CHS1, a TIR-NB protein, induces chilling sensitivity in Arabidopsis.

    Science.gov (United States)

    Wang, Yuancong; Zhang, Yao; Wang, Zheng; Zhang, Xiaoyan; Yang, Shuhua

    2013-08-01

    Low temperature is an environmental factor that affects plant growth and development and plant-pathogen interactions. How temperature regulates plant defense responses is not well understood. In this study, we characterized chilling-sensitive mutant 1 (chs1), and functionally analyzed the role of the CHS1 gene in plant responses to chilling stress. The chs1 mutant displayed a chilling-sensitive phenotype, and also displayed defense-associated phenotypes, including extensive cell death, the accumulation of hydrogen peroxide and salicylic acid, and an increased expression of PR genes: these phenotypes indicated that the mutation in chs1 activates the defense responses under chilling stress. A map-based cloning analysis revealed that CHS1 encodes a TIR-NB-type protein. The chilling sensitivity of chs1 was fully rescued by pad4 and eds1, but not by ndr1. The overexpression of the TIR and NB domains can suppress the chs1-conferred phenotypes. Interestingly, the stability of the CHS1 protein was positively regulated by low temperatures independently of the 26S proteasome pathway. This study revealed the role of a TIR-NB-type gene in plant growth and cell death under chilling stress, and suggests that temperature modulates the stability of the TIR-NB protein in Arabidopsis.

  4. Design of multichord Hα detector arrays for the U-3M torsatron and identification of rotating plasma perturbations

    Science.gov (United States)

    Dreval, M. B.; Shapoval, A. M.; Ozherelyev, F. I.; Makhov, M. M.

    2016-07-01

    An Hα camera has been designed and installed in the U-3M torsatron for spatially and temporally resolved measurements. This device provides fast measurements of the emission brightness profile in the noisy environment of the radio frequency (RF) heated plasma. Unusual topology of diagnostics and the data acquisition system are applied. All the system components, including digitizers, are assembled in a single unit. It allows the suppression of a low-frequency electromagnetic interference by eliminating the ground loops. And the suppression of RF noises is achieved by eliminating the signal interface cables and digital interface cables in the design. The Wi-Fi interface is used to prevent a ground loop in the data transfer stage. The achieved sensitivity of our diagnostics is high enough for measuring the Hα emission from the low-density (ne ≈ (1-2)ṡ1010 cm-3) plasma with a temporal resolution of about 20 μs in the noisy environment. Different types of Hα emission fluctuations within the frequency range of 1-5 kHz and poloidal mode numbers m = 0 and m = 5 have been observed in U-3M. A simple technique of the line-of-sight data analysis, based on the U-3M magnetic surface asymmetry, is proposed and used for the spatial localization of the rotating mode and for the determination of mode numbers and its poloidal rotation direction using a single Hα array.

  5. Testing of a pulsed He supersonic beam for plasma edge diagnostic in the TJ-IU torsatron

    Science.gov (United States)

    Tabarés, F. L.; Tafalla, D.; Herrero, V.; Tanarro, I.

    1997-02-01

    A new, compact atomic beam source based on the supersonic expansion of He has been developed for application as a plasma edge diagnostic. The beam is produced from a pulsed valve with a duration between 0.2 to 2 ms and a nominal repetition rate 10 and a divergence of ± 1° have been achieved at stagnation pressures below 2 bar. The diagnostic has been tested in ECRH plasmas on the TJ-IU torsatron, representing the first application of a supersonic beam to plasma characterization, to our knowledge. Operational conditions which minimized the total amount of He injected into the plasma were chosen. Non-perturbative injection conditions in the low density plasmas could be obtained at local He densities of ⋍ 1 × 10 11 cm -3 and a beam diameter < 1 cm. Due to the relatively low electron density of the ECRH plasmas, and to the good penetration characteristics of the supersonic He beam, the diagnostic could be used up to fairly low values of the normalized plasma minor radius, {r}/{a} (a = 12 cm) . Details of the optimization of the atomic beam diagnostics and typical results for steady state conditions in the TJ-IU plasmas are presented.

  6. Testing of a pulsed He supersonic beam for plasma edge diagnostic in the TJ-IU torsatron

    Energy Technology Data Exchange (ETDEWEB)

    Tabares, F.L. [Association EURATOM/CIEMAT, Madrid (Spain); Tafalla, D. [Association EURATOM/CIEMAT, Madrid (Spain); Herrero, V. [Instituto de Estructura de la Materia, CSIC, 28006 Madrid (Spain); Tanarro, I. [Instituto de Estructura de la Materia, CSIC, 28006 Madrid (Spain)

    1997-02-01

    A new, compact atomic beam source based on the supersonic expansion of He has been developed for application as a plasma edge diagnostic. The beam is produced from a pulsed valve with a duration between 0.2 to 2 ms and a nominal repetition rate <500 Hz. A terminal speed ratio >10 and a divergence of {+-}1 have been achieved at stagnation pressures below 2 bar. The diagnostic has been tested in ECRH plasmas on the TJ-IU torsatron, representing the first application of a supersonic beam to plasma characterization, to our knowledge. Operational conditions which minimized the total amount of He injected into the plasma were chosen. Non-perturbative injection conditions in the low density plasmas could be obtained at local He densities of {approx_equal}1 x 10{sup 11} cm{sup -3} and a beam diameter <1 cm. Due to the relatively low electron density of the ECRH plasmas, and to the good penetration characteristics of the supersonic He beam, the diagnostic could be used up to fairly low values of the normalized plasma minor radius, r/a (a=12 cm). Details of the optimization of the atomic beam diagnostics and typical results for steady state conditions in the TJ-IU plasmas are presented. (orig.).

  7. Edge plasma control using an LID configuration on CHS

    Energy Technology Data Exchange (ETDEWEB)

    Masuzaki, S.; Komori, A.; Morisaki, T. [National Inst. for Fusion Science, Oroshi, Toki (Japan)] [and others

    1997-07-01

    A Local Island Divertor (LID) has been proposed to enhance energy confinement through neutral particle control. For the case of the Large Helical Device (LHD), the separatrix of an m/n = 1/1 magnetic island, formed at the edge region, will be utilized as a divertor configuration. The divertor head is inserted in the island, and the island separatrix provides connection between the edge plasma region surrounding the core plasma and the back plate of the divertor head through the field lines. The particle flux and associated heat flux from the core plasma strike the back plate of the divertor head, and thus particle recycling is localized in this region. A pumping duct covers the divertor head to form a closed divertor system for efficient particle exhaust. The advantages of the LID are ease of hydrogen pumping because of the localized particle recycling and avoidance of the high heat load that would be localized on the leading edge of the divertor head. With efficient pumping, the neutral pressure in the edge plasma region will be reduced, and hence the edge plasma temperature will be higher, hopefully leading to a better core confinement region. A LID configuration experiment was done on the Compact Helical System (CHS) to confirm the effect of the LID. The typical effects of the LID configuration on the core plasma are reduction of the line averaged density to a half, and small or no reduction of the stored energy. In this contribution, the experimental results which were obtained in edge plasma control experiments with the LID configuration in the CHS are presented.

  8. The chitin synthase genes chs-1 and chs-2 are essential for C. elegans development and responsible for chitin deposition in the eggshell and pharynx, respectively.

    Science.gov (United States)

    Zhang, Yinhua; Foster, Jeremy M; Nelson, Laura S; Ma, Dong; Carlow, Clotilde K S

    2005-09-15

    It is widely accepted that chitin is present in nematodes. However, its precise role in embryogenesis is unclear and it is unknown if chitin is necessary in other nematode tissues. Here, we determined the roles of chitin and the two predicted chitin synthase genes in Caenorhabditis elegans by chitin localization and gene disruption. Using a novel probe, we detected chitin in the eggshell and discovered elaborate chitin localization patterns in the pharyngeal lumen walls. Chitin deposition in these two sites is likely regulated by the activities of chs-1 (T25G3.2) and chs-2 (F48A11.1), respectively. Reducing chs-1 gene activity by RNAi led to eggs that were fragile and permeable to small molecules, and in the most severe case, absence of embryonic cell division. Complete loss of function in a chs-1 deletion resulted in embryos that lacked chitin in their eggshells and failed to divide. These results showed that eggshell chitin provides both mechanical support and chemical impermeability essential to developing embryos. Knocking down chs-2 by RNAi caused a defect in the pharynx and led to L1 larval arrest, indicating that chitin is involved in the development and function of the pharynx.

  9. Chitin synthase 1 (Chs1) gene sequences of Microsporum equinum and Trichophyton equinum.

    Science.gov (United States)

    Kano, R; Aihara, S; Nakamura, Y; Watanabe, S; Hasegawa, A

    2001-01-05

    Chitin synthase 1 (Chs1) genes from Microsporum equinum and Trichophyton equinum were compared with those of the other dermatophytes. The Chs1 nucleotide sequences of these dermatophytes from horses showed more than 80% similarity to those of Arthroderma benhamiae, A. fulvum, A. grubyi, A. gypseum, A. incruvatum, A. otae, A. simii, A. vanbreuseghemii, Epidermophyton floccosum, T. mentagrophytes var. interdigitale (T. interdigitale), T. rubrum and T. violaceum. Especially high degree of nucleotide sequence similarity of more than 99% was noted between the Chs1 gene fragments of M. equinum and A. otae, and those of T. equinum, T. interdigitale and A. vanbreuseghemii, respectively. The phylogenetic analysis of their sequences revealed that M. equinum was genetically very close to A. otae and T. equinum to A. vanbreuseghemii. A molecular analysis of Chs1 genes will provide useful information for the genetic relatedness of M. equinum and T. equinum and confirm the value of DNA sequencing in identification of these two dermatophytes.

  10. A Malus crabapple chalcone synthase gene, McCHS, regulates red petal color and flavonoid biosynthesis.

    Directory of Open Access Journals (Sweden)

    Deqiang Tai

    Full Text Available Chalcone synthase is a key and often rate-limiting enzyme in the biosynthesis of anthocyanin pigments that accumulate in plant organs such as flowers and fruits, but the relationship between CHS expression and the petal coloration level in different cultivars is still unclear. In this study, three typical crabapple cultivars were chosen based on different petal colors and coloration patterns. The two extreme color cultivars, 'Royalty' and 'Flame', have dark red and white petals respectively, while the intermediate cultivar 'Radiant' has pink petals. We detected the flavoniods accumulation and the expression levels of McCHS during petals expansion process in different cultivars. The results showed McCHS have their special expression patterns in each tested cultivars, and is responsible for the red coloration and color variation in crabapple petals, especially for color fade process in 'Radiant'. Furthermore, tobacco plants constitutively expressing McCHS displayed a higher anthocyanins accumulation and a deeper red petal color compared with control untransformed lines. Moreover, the expression levels of several anthocyanin biosynthetic genes were higher in the transgenic McCHS overexpressing tobacco lines than in the control plants. A close relationship was observed between the expression of McCHS and the transcription factors McMYB4 and McMYB5 during petals development in different crabapple cultivars, suggesting that the expression of McCHS was regulated by these transcription factors. We conclude that the endogenous McCHS gene is a critical factor in the regulation of anthocyanin biosynthesis during petal coloration in Malus crabapple.

  11. Autoimmunity conferred by chs3-2D relies on CSA1, its adjacent TNL-encoding neighbour.

    Science.gov (United States)

    Xu, Fang; Zhu, Chipan; Cevik, Volkan; Johnson, Kaeli; Liu, Yanan; Sohn, Kee; Jones, Jonathan D; Holub, Eric B; Li, Xin

    2015-03-05

    Plant innate immunity depends on the function of a large number of intracellular immune receptor proteins, the majority of which are structurally similar to mammalian nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) proteins. CHILLING SENSITIVE 3 (CHS3) encodes an atypical Toll/Interleukin 1 Receptor (TIR)-type NLR protein with an additional Lin-11, Isl-1 and Mec-3 (LIM) domain at its C-terminus. The gain-of-function mutant allele chs3-2D exhibits severe dwarfism and constitutively activated defense responses, including enhanced resistance to virulent pathogens, high defence marker gene expression, and salicylic acid accumulation. To search for novel regulators involved in CHS3-mediated immune signaling, we conducted suppressor screens in the chs3-2D and chs3-2D pad4-1 genetic backgrounds. Alleles of sag101 and eds1-90 were isolated as complete suppressors of chs3-2D, and alleles of sgt1b were isolated as partial suppressors of chs3-2D pad4-1. These mutants suggest that SAG101, EDS1-90, and SGT1b are all positive regulators of CHS3-mediated defense signaling. Additionally, the TIR-type NLR-encoding CSA1 locus located genomically adjacent to CHS3 was found to be fully required for chs3-2D-mediated autoimmunity. CSA1 is located 3.9 kb upstream of CHS3 and is transcribed in the opposite direction. Altogether, these data illustrate the distinct genetic requirements for CHS3-mediated defense signaling.

  12. Novel Heterogenous CHS1 Mutations Identified in Five Japanese Patients with Chediak-Higashi Syndrome.

    Science.gov (United States)

    Tanabe, Fuminori; Kasai, Hirotake; Morimoto, Michiko; Oh, Shigeharu; Takada, Hidetoshi; Hara, Toshiro; Ito, Masahiko

    2010-01-01

    Chediak-Higashi syndrome (CHS) is a rare, autosomal recessive disorder characterized by oculocutaneous albinism, recurrent bacterial infections and progressive neurological dysfunction. We demonstrate novel heterogenous mutations of CHS1, the responsive gene of CHS, identified in five Japanese patients with CHS. Patients 1, 2, and 3 were siblings, and they had albinism of the skin and hair. They all had a heterogenous two-base deletion (c.5541-5542 del AA, p.Q1847fsX1850) in exon 18. Patient 4 had a heterogenous single-base insertion (c.3944-3945 ins C, p.T1315fsX1331) in exon 10. The patient exhibited severe early-onset phenotype and suffered from hemophagocytic lymphohistiocytosis. Patient 5 had two heterogenous nonsense mutations; c.7982C>G, p.S2661X in exon 30 and c.8281A>T, p.R2761X in exon 31. The patient suffered from infections in childhood and had visual disturbance and albinism of the skin and hair. The CHS1 mutations described here have not been reported previously.

  13. RNA interference silencing of CHS greatly alters the growth pattern of apple (Malus x domestica).

    Science.gov (United States)

    Dare, Andrew P; Hellens, Roger P

    2013-08-01

    Plants produce a vast array of phenolic compounds which are essential for their survival on land. One major class of polyphenols are the flavonoids and their formation is dependent on the enzyme chalcone synthase (CHS). In a recent study we silenced the CHS genes of apple (Malus × domestica Borkh.) and observed a loss of pigmentation in the fruit skin, flowers and stems. More surprisingly, highly silenced lines were significantly reduced in size, with small leaves and shortened internode lengths. Chemical analysis also revealed that the transgenic shoots contained greatly reduced concentrations of flavonoids which are known to modulate auxin flow. An auxin transport study verified this, with an increased auxin transport in the CHS-silenced lines. Overall, these findings suggest that auxin transport in apple has adapted to take place in the presence of high endogenous concentrations of flavonoids. Removal of these compounds therefore results in abnormal auxin movement and a highly disrupted growth pattern.

  14. 黄皮 CHS 基因的克隆及其表达%Clone and Expression of CHS Gene in Wampee

    Institute of Scientific and Technical Information of China (English)

    张宇; 许真; 赵志常; 高爱平; 陈业渊; 黄建峰; 党志国; 罗睿雄

    2014-01-01

    In order to study the function of CHS gene,a CHS gene was cloned from wampee fruit by homology cloning method taking cDNA and DNA of wampee fruit as the template, then sequence comparison and clustering analysis was conducted.Results:The open reading frame was 1032 bp,and encoding 343 amino acids.The 1100bp fragment was amplified from the genome,the gene did not contain introns.The expression of CHS gene was mainly in fruit,and in flowers was higher than in leaves.It was inferred that the gene may be involved in the flavonoids synthesis of fruit and flowers.The cluster analysis showed that the gene encoding protein had near relationship with citrus,considered as a group with orange,grape,peony,litchi,longan,and other groups.%为研究黄皮果实中查尔酮合成酶(CHS)的功能,以黄皮果实的 cDNA 和 DNA 为模板,采用同源克隆的方法克隆得 CHS 基因后进行序列比对和聚类分析。结果表明:该基因的开放阅读框为1032 bp,编码343个氨基酸。基因组扩增得到了1100 bp 的片段,不含内含子。该基因主要在果实中表达,而叶片中表达较其他组织低,初步推断该基因可能与果实和花朵中的黄酮类物质合成有密切的关系。该基因编码的蛋白与柑桔的 CHS 蛋白序列亲缘关系较近,可与柑桔、葡萄、芍药、牡丹、荔枝、龙眼等聚为一类。

  15. Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA)

    Institute of Scientific and Technical Information of China (English)

    李艳; 惠有为; 张仲凯; 黄兴奇; 李毅

    2001-01-01

    Chalcone synthase A is a key enzyme in the anthocyanin biosynthesis pathway. Expression of chsA gene in transgenic Petunia hybrida resulted in flower color alterations and co-suppression of transgenes and endogenous genes. We fused the β-glucuronidase (uidA) gene to the C-terminal of chsA gene, and transferred the fusion gene into Petunia hybrida via Agrobacterium tumefaciens. GUS histochemical staining analysis showed that co-suppression occurred specifically during the development of flowers and co-suppression required the mutual interaction of endogenous genes and transgenes. RNA in situ hybridization analysis suggested that co-suppression occurred in the entire plant, and RNA degradation occurred in the cytoplasm.

  16. A TIR-NBS protein encoded by Arabidopsis Chilling Sensitive 1 (CHS1) limits chloroplast damage and cell death at low temperature.

    Science.gov (United States)

    Zbierzak, Anna Maria; Porfirova, Svetlana; Griebel, Thomas; Melzer, Michael; Parker, Jane E; Dörmann, Peter

    2013-08-01

    Survival of plants at low temperature depends on mechanisms for limiting physiological damage and maintaining growth. We mapped the chs1-1 (chilling sensitive1-1) mutation in Arabidopsis accession Columbia to the TIR-NBS gene At1g17610. In chs1-1, a single amino acid exchange at the CHS1 N-terminus close to the conserved TIR domain creates a stable mutant protein that fails to protect leaves against chilling stress. The sequence of another TIR-NBS gene (At5g40090) named CHL1 (CHS1-like 1) is related to that of CHS1. Over-expression of CHS1 or CHL1 alleviates chilling damage and enhances plant growth at moderate (24°C) and chilling (13°C) temperatures, suggesting a role for both proteins in growth homeostasis. chs1-1 mutants show induced salicylic acid production and defense gene expression at 13°C, indicative of autoimmunity. Genetic analysis of chs1-1 in combination with defense pathway mutants shows that chs1-1 chilling sensitivity requires the TIR-NBS-LRR and basal resistance regulators encoded by EDS1 and PAD4 but not salicylic acid. By following the timing of metabolic, physiological and chloroplast ultrastructural changes in chs1-1 leaves during chilling, we have established that alterations in photosynthetic complexes and thylakoid membrane integrity precede leaf cell death measured by ion leakage. At 24°C, the chs1-1 mutant appears normal but produces a massive necrotic response to virulent Pseudomonas syringae pv. tomato infection, although this does not affect bacterial proliferation. Our results suggest that CHS1 acts at an intersection between temperature sensing and biotic stress pathway activation to maintain plant performance over a range of conditions.

  17. Swelling and microstructure of austenitic stainless steel ChS-68 CW after high dose neutron irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Porollo, S.I.; Konobeev, Yu.V. [State Scientific Center of Russian Federation - Institute of Physics and Power Engineering (IPPE), Obninsk, Kaluga Region (Russian Federation); Garner, F.A., E-mail: frank.garner@dslextreme.co [Radiation Effects Consulting, 2003 Howell Avenue, Richland, WA 99354 (United States)

    2009-08-15

    Austenitic stainless steel ChS-68 serving as fuel pin cladding was irradiated in the 20% cold-worked condition in the BN-600 fast reactor in the range 56-84 dpa. This steel was developed to replace EI-847 which was limited by its insufficient resistance to void swelling. Comparison of swelling between EI-847 and ChS-68 under similar irradiation conditions showed improvement of the latter steel by an extended transient regime of an additional approx10 dpa. Concurrent with swelling was the development of a variety of phases. In the temperature range 430-460 deg. S where the temperature peak of swelling was located, the principal type of phase generated during irradiation was G-phase, with volume fraction increasing linearly with dose to approx0.5% at 84 dpa. While the onset of swelling is concurrent with formation of G-phase, the action of G-phase cannot be confidently ascribed to significant removal from solution of swelling-suppressive elements such as silicon. A plausible mechanism for the higher resistance to void swelling of ChS-68 as compared with EI-847 may be related to an observed higher stability of faulted dislocation loops in ChS-68 that impedes the formation of a glissile dislocation network. The higher level of boron in ChS-68 is thought to be one contributor that might play this role.

  18. Molecular evolution of the exon 2 of CHS genes and the possibility of its application to plant phylogenetic analysis

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The exon 2 of chalcone synthase (CHS) gene is relatively conserved during evolution.In this study,three exon 2 fragments from two species in gymnosperm (Cycas panzhihuaensis,Ginkgo biloba) and seven from four species in angiosperm (Magnolia denudata,Salix babylonica,Nymphaea tetragona,Camellia japonica) have been amplified by PCR from genomic DNA and sequenced.Together with other 73 sequences of CHS collected from EMBL database and literature,these sequences,which embrace 19 families of gymnosperm and angiosperm,have been analyzed for their phylogenetic relations by parsimony method.The result indicated that sequences from the same systematic family usually grouped together except those from Theaceae,Magnoliaceae and Nymphaeaceae.The relative rate test revealed the rate heterogeneity of CHS genes among the families.For the nucleotide substitution the sequences from Asteraceae and Solanaceae evolve faster than those from the other families analyzed while the sequences from Poaceae,Asteraceae and Solanaceae evolve faster for the nonsynonymous substitution.These results suggest that the duplication and extinction events of CHS genes are different among systematic families,therefore it seems impractical to look for orthologous sequences from CHS genes to study plant phylogeny at the family level and/or above.However,it is possible to do so below the family level.

  19. Changes of the electron density distribution during MHD activity in CHS

    Energy Technology Data Exchange (ETDEWEB)

    Soltwish, H. [Ruhr-University Bochum, Institute for Experimental Physics, Bochum (Germany); Tanaka, K. [National Inst. for Fusion Science, Toki, Gifu (Japan)

    2000-09-01

    Density oscillations induced by MHD activities were observed in NBI heated plasmas on CHS by using an HCN laser interferometer. The accompanied changes of the density profiles were also observed. The oscillations are composition of m=0 sawteeth like crash and m=2 sinusoidal oscillations as a post courser of the crash. Possible models of the oscillation structure are examined in order to explain experimental data of the interferometer. Rotating plasma core, which is hollow profile and keeps constant elongation of the flux surface can explain amplitude and phase distribution of the sinusoidal oscillation. (author)

  20. 90Y-DOTA-CHS Microspheres for Live Radiomicrosphere Therapy: Preliminary In Vivo Lung Radiochemical Stability Studies

    Directory of Open Access Journals (Sweden)

    Alejandro Amor-Coarasa

    2014-01-01

    Full Text Available Chitosan (CHS is used to prepare microspheres of 31 ± 8 µm size. Surface modification with p-SCN-Bn-DOTA was performed. A maximum 90Y capacity was found to be 12.1 ± 4.4 µCi/particle. The best obtained labeling yield was 87.7 ± 0.6%. More than 90% in vitro stability was found. Particle in vitro degradation half-life in PBS was found to be greater than 21 days. In vivo studies with 90Y-DOTA-CHS showed more than 95% of the injected activity (decay corrected in the lungs 24 hours after tail vein administration. 90Y-DOTA-CHS in vivo label stability was superior to resin microspheres. The addition of p-SCN-Bn-DOTA served as a radioprotectant for bone marrow as the 5% 90Y released, during the first 24 hours, was quickly eliminated via urine.

  1. CHS silencing suggests a negative cross-talk between wax and flavonoid pathways in tomato fruit cuticle.

    Science.gov (United States)

    Heredia, Antonio; Heredia-Guerrero, José Alejandro; Domínguez, Eva

    2015-01-01

    Tomato fruits (Solanum lycopersicum L.) accumulate flavonoids in their cuticle and epidermal cells during ripening. These flavonoids come from de novo biosynthesis due to a significant increase in chalcone synthase (CHS) activity during ripening. Virus-induced gene silencing (VIGS) of tomato fruits have been used to down-regulate SlCHS expression during ripening and analyze the effects at the epidermal and cuticle level. Besides the expected change in fruit color due to a lack of flavonoids incorporated to the cuticle, several other modifications such as a decrease in the amount of cutin and polysaccharides were observed. These indicate a role for either flavonoids or CHS in the alteration of the expression levels of some genes involved in cuticle biosynthesis. Moreover, a negative interaction between the 2 cuticle components, flavonoids and waxes, suggests a relationship between these 2 metabolic pathways.

  2. Parametric analysis and design equation of ultimate capacity for unstiffened overlapped CHS K-joints

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A finite element model simulating an experiment on unstiffened,overlapped circular hollow structure (CHS)K-joints was generated and validated by comparing the ultimate capacities,deformation processes and failure modes of the experimental results.Using this model,the stress distribution,propagation of plasticity and the failure modes of overlapped joints with through-brace-in-compression and welded hidden seams were analyzed.The effect of geometric parameters,with or without hidden welds,and the loading hierarchy reversal of braces on the ultimate capacity of the joints were also studied.The results of finite element parametric analysis indicate that the brace-tochord thickness ratio has relatively large effects on the failure mechanism and ultimate capacity of overlapped joints.It was also found that the absence of hidden welds has less significance on the ultimate capacity of through-brace-incompression joints than through-brace-in tension joints.Finally,based on the design equation of gap joints,a formula predicting the ultimate capacity of overlapped CHS K-joints was derived by applying multivariate regression analysis.Results from the proposed design equation are consistent with experimental results.

  3. RELATIVE EXPRESSION OF GENES CHIA1, SGF14C AND CHS8* IN SOYBEAN SEED COATS

    Directory of Open Access Journals (Sweden)

    CARLOS ANDRÉ BAHRY

    2014-01-01

    Full Text Available The aim of this study was to evaluate the relative expression of three candidate genes, CHIA1, SGF14c and CHS8 * possibly involved in seed quality, in contrasting seed coats from four soybean genotypes. Two genotypes with yellow seed coats, BMX Potência RR and CD 202, and two genotypes with black seed coats, TP and IAC were studied to determine the relative gene expression through the qPCR technique, in sev- en stages of seed coat development for all four genotypes, at 25, 30, 35, 40, 45, 50 and 55 days after anthesis. The CHIA1 and SGF14c genes showed higher expression in cultivar CD 202; the former in the final stages of seed coat development, at 55 days after anthesis, the latter gene at earlier stages, specifically at 25 days after anthesis. The CHS8* gene showed higher expression in CD 202 seed coats at 50 days after anthesis. All three genes expressed at higher levels on genotypes of yellow seed coats, and are considered relevant to new areas of research based on the expression of genes related to seed quality.

  4. Cloning and Expression Analysis of ChCHS1 and ChDFR1 Genes From Cattleya%卡特兰ChCHS1和ChDFR1基因的克隆及表达分析

    Institute of Scientific and Technical Information of China (English)

    王紫珊; 周琳; 王雁

    2015-01-01

    以卡特兰紫色花品种‘粉女郎’(Cattleya hybrid ‘Pink Lady’)为材料,采用RT-PCR和RACE方法从花瓣中分离得到了一个查尔酮合酶(chalcone synthase,CHS)和一个二氢黄酮醇4-还原酶(dihydroflavonol 4-reductase,DFR)同源基因的cDNA全长,分别命名为ChCHS1和ChDFR,GenBank登录号分别为KP171693和KP171694.序列分析结果发现:ChCHS1的cDNA全长l 508 bp,编码394个氨基酸;ChDFR1基因的cDNA全长1 250 bp,编码350个氨基酸.同源性检索和生物信息学分析表明,这2个基因编码的蛋白都具有各自的功能位点和保守性特征多肽序列.氨基酸序列比对与系统进化树分析显示,ChCHS1、ChDFR1基因在兰科中与蕙兰、石斛兰关系最近,与百合科关系较近.相对实时荧光定量PCR结果表明,ChCHS1和ChDFR1都在蕾期表达量最低,伴随花朵的开放,表达量逐渐上升,最终分别在花朵盛开期和接近开放时达到最高.

  5. The spectral type of CHS7797 - an intriguing very low mass periodic variable in the Orion Nebula Cluster

    CERN Document Server

    Rodriguez-Ledesma, M V; Pintado, O; Boudreault, S; Hessman, F; Herbst, W

    2013-01-01

    We present the spectroscopic characterization of the unusual high-amplitude very low mass pre-main-sequence periodic variable CHS7797. This study is based on optical medium-resolution (R=2200) spectroscopy in the 6450-8600 A range, carried out with GMOS-GEMINI-S in March 2011. Observations of CHS7797 have been carried out at two distinct phases of the 17.8d period, namely at maximum and four days before maximum. Four different spectral indices were used for the spectral classification at these two phases, all of them well-suited for spectral classification of young and obscured late M dwarfs. In addition, the gravity-sensitive NaI (8183/8195 A) and KI (7665/7699 A) doublet lines were used to confirm the young age of CHS7797. From the spectrum obtained at maximum light we derived a spectral type (SpT) of M6.05, while for the spectrum taken four days before maximum the derived SpT is M5.75. The derived SpTs confirm that CHS7797 has a mass in the stellar-substellar boundary mass range. In addition, the small dif...

  6. The impact of cHS4 insulators on DNA transposon vector mobilization and silencing in retinal pigment epithelium cells.

    Directory of Open Access Journals (Sweden)

    Nynne Sharma

    Full Text Available DNA transposons have become important vectors for efficient non-viral integration of transgenes into genomic DNA. The Sleeping Beauty (SB, piggyBac (PB, and Tol2 transposable elements have distinct biological properties and currently represent the most promising transposon systems for animal transgenesis and gene therapy. A potential obstacle, however, for persistent function of integrating vectors is transcriptional repression of the element and its genetic cargo. In this study we analyze the insulating effect of the 1.2-kb 5'-HS4 chicken β-globin (cHS4 insulator element in the context of SB, PB, and Tol2 transposon vectors. By examining transgene expression from genomically inserted transposon vectors encoding a marker gene driven by a silencing-prone promoter, we detect variable levels of transcriptional silencing for the three transposon systems in retinal pigment epithelium cells. Notably, the PB system seems less vulnerable to silencing. Incorporation of cHS4 insulator sequences into the transposon vectors results in 2.2-fold and 1.5-fold increased transgene expression levels for insulated SB and PB vectors, respectively, but an improved persistency of expression was not obtained for insulated transgenes. Colony formation assays and quantitative excision assays unveil enhanced SB transposition efficiencies by the inclusion of the cHS4 element, resulting in a significant increase in the stable transfection rate for insulated SB transposon vectors in human cell lines. Our findings reveal a positive impact of cHS4 insulator inclusion for SB and PB vectors in terms of increased transgene expression levels and improved SB stable transfection rates, but also the lack of a long-term protective effect of the cHS4 insulator against progressive transgene silencing in retinal pigment epithelium cells.

  7. A single-repeat MYB transcription factor, GmMYB176, regulates CHS8 gene expression and affects isoflavonoid biosynthesis in soybean.

    Science.gov (United States)

    Yi, Jinxin; Derynck, Michael R; Li, Xuyan; Telmer, Patrick; Marsolais, Frédéric; Dhaubhadel, Sangeeta

    2010-06-01

    Here we demonstrate that GmMYB176 regulates CHS8 expression and affects isoflavonoid synthesis in soybean. We previously established that CHS8 expression determines the isoflavonoid level in soybean seeds by comparing the transcript profiles of cultivars with different isoflavonoid contents. In the present study, a functional genomic approach was used to identify the factor that regulates CHS8 expression and isoflavonoid synthesis. Candidate genes were cloned, and co-transfection assays were performed in Arabidopsis leaf protoplasts. The results showed that GmMYB176 can trans-activate the CHS8 promoter with maximum activity. Transient expression of GmMYB176 in soybean embryo protoplasts increased endogenous CHS8 transcript levels up to 169-fold after 48 h. GmMYB176 encodes an R1 MYB protein, and is expressed in soybean seed during maturation. Furthermore, GmMYB176 recognizes a 23 bp motif containing a TAGT(T/A)(A/T) sequence within the CHS8 promoter. A subcellular localization study confirmed nuclear localization of GmMYB176. A predicted pST binding site for 14-3-3 protein is required for subcellular localization of GmMYB176. RNAi silencing of GmMYB176 in hairy roots resulted in reduced levels of isoflavonoids, showing that GmMYB176 is necessary for isoflavonoid biosynthesis. However, over-expression of GmMYB176 was not sufficient to increase CHS8 transcript and isoflavonoid levels in hairy roots. We conclude that an R1 MYB transcription factor, GmMYB176, regulates CHS8 expression and isoflavonoid synthesis in soybean.

  8. A novel CHS/ALG bi-layer composite membrane with sustained antimicrobial efficacy used as wound dressing

    Institute of Scientific and Technical Information of China (English)

    Yang Dong; Hong Zhuo Liu; Lu Xu; Gang Li; Zi Ning Ma; Fei Han; Hui Min Yao; Yan Hui Sun; San Ming Li

    2010-01-01

    A membrane composed of an alginate (ALG) layer and a chitosan (CHS) layer with sustained antimicrobial efficacy was prepared. Ciprofloxacin HCI (CIP) was incorporated into the ALG layer. Morphological feature of the composite membrane was analyzed by scanning electron microscopy (SEM). Water uptake capacity, in vitro drug release, and in vitro antimicrobial activity were evaluated. The composite membrane exhibited perfect binding characteristic between the two layers. The water uptake capacity of all the membranes was above 800%. The CIP could release from the composite membranes for 48 h. The membrane could control the bacterial growth persistently. The results suggested that this CHS/ALG composite membrane incorporated with CIP had the potential for wound dressing application.

  9. The cHS4 insulator increases the probability of retroviral expression at random chromosomal integration sites.

    Science.gov (United States)

    Rivella, S; Callegari, J A; May, C; Tan, C W; Sadelain, M

    2000-05-01

    Retroviruses are highly susceptible to transcriptional silencing and position effects imparted by chromosomal sequences at their integration site. These phenomena hamper the use of recombinant retroviruses as stable gene delivery vectors. As insulators are able to block promoter-enhancer interactions and reduce position effects in some transgenic animals, we examined the effect of an insulator on the expression and structure of randomly integrated recombinant retroviruses. We used the cHS4 element, an insulator from the chicken beta-like globin gene cluster, which has been shown to reduce position effects in transgenic Drosophila. A large panel of mouse erythroleukemia cells that bear a single copy of integrated recombinant retroviruses was generated without using drug selection. We show that the cHS4 increases the probability that integrated proviruses will express and dramatically decreases the level of de novo methylation of the 5' long terminal repeat. These findings support a primary role of methylation in the silencing of retroviruses and suggest that cHS4 could be useful in gene therapy applications to overcome silencing of retroviral vectors.

  10. Characterization of a midgut-specific chitin synthase gene (LmCHS2) responsible for biosynthesis of chitin of peritrophic matrix in Locusta migratoria.

    Science.gov (United States)

    Liu, Xiaojian; Zhang, Huanhuan; Li, Sheng; Zhu, Kun Yan; Ma, Enbo; Zhang, Jianzhen

    2012-12-01

    Chitin, an essential component of peritrophic matrix (PM), is produced by a series of biochemical reactions. Chitin synthase plays a crucial role in chitin polymerization in chitin biosynthetic pathway. In this study, we identified and characterized a full-length cDNA of chitin synthase 2 gene (LmCHS2) from Locusta migratoria. The cDNA contains an open reading frame of 4569 nucleotides that encode 1523 amino acid residues, and 76- and 373-nucleotides for 5'- and 3'-noncoding regions, respectively. Analysis of LmCHS2 transcript in different tissues of the locust by using real-time quantitative PCR indicated that LmCHS2 was exclusively expressed in midgut and gastric caeca (a part of the midgut). The highest expression was found in the anterior midgut with a decline of the transcript level from the anterior to posterior regions. During growth and development of locusts, there was only a slight expression in eggs, but the expression gradually increased from nymphs to adults. In situ hybridization further revealed that LmCHS2 transcript mainly presented in the apical regions of brush border forming columnar cells of gastric caeca. LmCHS2 dsRNA was injected to fifth-instar nymphs to further explore biological functions of LmCHS2. Significantly down-regulated transcript of LmCHS2 resulted in a cessation of feeding and a high mortality of the insect. However, no visible abnormal morphological change of locusts was observed until insects molted to adults. After dissection, we found that the average length of midguts from the LmCHS2 dsRNA-injected locusts was shorter than that of the control insects that were injected with dsGFP. Furthermore, microsection of midguts showed that the PM of the LmCHS2 dsRNA-injected nymphs was amorphous and thin as compared with the controls. Our results demonstrate that LmCHS2 is responsible for the biosynthesis of chitin associated with PM and plays an essential role in locust growth and development.

  11. A new member of the chalcone synthase (CHS family in sugarcane

    Directory of Open Access Journals (Sweden)

    Contessotto Miriam G.G.

    2001-01-01

    Full Text Available Sequences from the sugarcane expressed sequence tag (SUCEST database were analyzed based on their identities to genes encoding chalcone-synthase-like enzymes. The sorghum (Sorghum bicolor chalcone-synthase (CHS, EC 2.3.1.74 protein sequence (gi|12229613 was used to search the SUCEST database for clusters of sequencing reads that were most similar to chalcone synthase. We found 121 reads with homology to sorghum chalcone synthase, which we were then able to sort into 14 clusters which themselves were divided into two groups (group 1 and group 2 based on the similarity of their deduced amino acid sequences. Clusters in group 1 were more similar to the sorghum enzyme than those in group 2, having the consensus sequence of the active site of chalcone and stilbene synthase. Analysis of gene expression (based on the number of reads from a specific library present in each group indicated that most of the group 1 reads were from sugarcane flower and root libraries. Group 2 clusters were more similar to the amino acid sequence of an uncharacterized pathogen-induced protein (PI1, gi|9855801 from the S. bicolor expressed sequence tag (EST database. The group 2 clusters sequences and PI1 proteins are 90% identical, having two amino acid changes at the chalcone and stilbene synthase consensi but conserving the cysteine residue at the active site. The PI1 EST has not been previously associated with chalcone synthase and has a different consensus sequence from the previously described chalcone synthase of sorghum. Most of the group 2 reads were from libraries prepared from sugarcane roots and plants infected with Herbaspirillum rubrisubalbicans and Gluconacetobacter diazotroficans. Our results indicate that we have identified a sugarcane chalcone synthase similar to the pathogen-induced PI1 protein found in the sorghum cDNA libraries, and it appears that both proteins represent new members of the chalcone and stilbene synthase super-family.

  12. CHS Application In Automotive wiring Harness Design%Capital Harness XC线束设计软件的应用

    Institute of Scientific and Technical Information of China (English)

    王春芝

    2015-01-01

    Capital Harness XC是Captial Harness System (CHS)软件中一个设计模块,本文介绍了Capital Harness XC线束设计模块的应用,及以Capital Harness XC在设计流程中的作用和使用过程中应注意的事项,使读者能够理解Capital Harness XC在汽车设计中的操作应用方法.同时指出其在设计过程中的优点及在实际汽车领域中的意义.

  13. El conflicto colombo-peruano y las reacciones del Centro de Historia de Santander (CHS, 1932-1937

    Directory of Open Access Journals (Sweden)

    Monika Liliana Gonzalez

    2012-12-01

    Full Text Available El artículo examina y describe las reacciones que tuvo el Centro de Historia de Santander (CHS, tras el inicio del conflicto con el Perú en 1932. En la primera parte del trabajo se caracteriza sucintamente la llamada Guerra con el Perú (1932-1933 con el fin de ubicar al lector en los acontecimientos que generaron la movilización de un grupo de hombres de letras en Bucaramanga (Colombia en la defensa simbólica de su Patria. En la segunda, se procura mostrar cómo una entidad cultural dedicada a la creación del pasado regional, como fue el CHS, desarrolló una serie de eventos, actividades y publicaciones que dan cuenta de un uso político del pasado nacional. Con ello, sus miembros cumplieron el objetivo misional de defender la integridad territorial de Colombia y difundir el sentimiento patriótico en la sociedad santandereana.

  14. The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum.

    Science.gov (United States)

    Gandía, Mónica; Harries, Eleonora; Marcos, Jose F

    2014-06-01

    Chitin is an essential component of the fungal cell wall and a potential target in the development of new antifungal compounds, due to its presence in fungi and not in plants or vertebrates. Chitin synthase genes (chs) constitute a complex family in filamentous fungi and are involved in fungal development, morphogenesis, pathogenesis and virulence. In this study, additional chs genes in the citrus postharvest pathogen Penicillium digitatum have been identified. Comparative analyses included each PdChs in each one of the classes I to VII previously established, and support the grouping of these into three divisions. Disruption of the gene coding PdChsVII, which contains a short version of a myosin motor domain, has been achieved by using Agrobacterium tumefaciens-mediated transformation and revealed its role in the life cycle of the fungus. Disruption strains were viable but showed reduced growth and conidia production. Moreover, Pdchs mutants developed morphological defects as balloon-like enlarged cells and increased chitin content, indicative of an altered cell wall structure. Gene disruption also increased susceptibility to antifungal compounds such as calcofluor white (CFW), sodium dodecyl sulfate (SDS), hydroxide peroxide (H2O2) and commercial fungicides, but significantly no change was observed in the sensitivity to antifungal peptides. The PdchsVII mutants were able to infect citrus fruit and produced tissue maceration, although had reduced virulence and most importantly were greatly impaired in the production of visible mycelium and conidia on the fruit.

  15. Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA)

    Institute of Scientific and Technical Information of China (English)

    LI; Yan; (

    2001-01-01

    [1]Napoli, C., Lemieux, C., Jorgensen, R., Introduction of a chimeric chalone synthase gene into petunia results in reversible cosuppession of homologous genes in trans, The Plant Cell, 1990, 2: 279-289.[2]Van der Krol, A.R., Mur, L.A., Beld, M. M. et al., Flavonnoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression, The Plant Cell, 1990, 2: 291-299.[3]Manika, P.B., Bhadra, U., Birchler, J., Cosuppression in Drosophila: gene silencing of Alcohol dehydrogenase by White-Adh transgene is Polycomb dependent, Cell, 1997, 90: 479-498.[4]de Carvalho Niebel, F., Frendo, P., Van Montagu, M. et al., Post-transcriptional cosuppression of ?-1,3-glucanase transgene expression in homozygous plants, EMBO J., 1992, 11: 2595-2602.[5]Van Blokland, R., Van der Geest, N., Mol, J. N. M. et al., Transgene-mediated suppression of chalcone synthase expression in Petunia hybrida results from an increase in RNA turnover, The Plant Cell, 1994, 6: 861-877.[6]Stam, M., Mol, J. N. M., Kooter, J. M., The silence of genes in transgenic plants, Annals of Bot., 1997, 79: 3-12.[7]Vaucheret, H., Beclin, C., Elmayan, T. et al., Transgene-induced gene silencing in plants, Plant J., 1998, 16(6): 651-659.[8]Shao, L., Li, Y., Yang, M. Z. et al., Transformation of Petunia hybrida with chalcone synthase A (chsA) resulting flower colour alteration and male sterility, Acta Botanica Sinica (in Chinese), 1996, 38(7): 517-524.[9]Koes, R. E., Spelt, C. E., Mol, J. N. M., The chalcone synthase multigene family of Petunia hybrida (V30): differential, light-regulated expression during flower development and UV light induction, Plant Mol. Biol., 1989, 12: 213-225.[10]Drews, G. N., Beals, T. P., Bul, A. Q. et al., Regional and cell-specific expression patterns during petal development, The Plant Cell, 1992, 4: 1383-1404.[11]Martin, C., Gerats, T., Control of pigment biosynthesis genes during petal development, The

  16. A facile in situ self-assembly strategy for large-scale fabrication of CHS@MOF yolk/shell structure and its catalytic application in a flow system.

    Science.gov (United States)

    Gao, Hongyi; Luan, Yi; Chaikittikul, Kullapat; Dong, Wenjun; Li, Jie; Zhang, Xiaowei; Jia, Dandan; Yang, Mu; Wang, Ge

    2015-03-04

    A hierarchical yolk/shell copper hydroxysulfates@MOF (CHS@MOF, where MOF = metal-organic frameworks) structure was fabricated from a homogeneous yolk/shell CHS template composed of an active shell and a stabilized core via a facile self-template strategy at room temperature. The active shell of the template served as the source of metal ion and was in situ transformed into a well-defined MOF crystal shell, and the relatively stabilized core retained its own nature during the formation of the MOF shell. The strategy of in situ transformation of CHS shell to MOF shell avoided the self-nucleation of MOF in the solution and complex multistep procedures. Furthermore, a flow reaction system using CHS@MOF as self-supported stationary-phase catalyst was developed, which demonstrated excellent catalytic performance for aldehyde acetalization with ethanol, and high yields and selectivities were achieved under mild conditions.

  17. Association of Smoking, Alcohol, and Obesity with Cardiovascular Death and Ischemic Stroke in Atrial Fibrillation: The Atherosclerosis Risk in Communities (ARIC) Study and Cardiovascular Health Study (CHS).

    Science.gov (United States)

    Kwon, Younghoon; Norby, Faye L; Jensen, Paul N; Agarwal, Sunil K; Soliman, Elsayed Z; Lip, Gregory Y H; Longstreth, W T; Alonso, Alvaro; Heckbert, Susan R; Chen, Lin Y

    2016-01-01

    Atrial fibrillation (AF) is associated with an increased risk of ischemic stroke and cardiovascular (CV) death. Whether modifiable lifestyle risk factors are associated with these CV outcomes in AF is unknown. Among Atherosclerosis Risk in Communities (ARIC) study and Cardiovascular Health Study (CHS) participants with incident AF, we estimated the risk of composite endpoint of ischemic stroke or CV death associated with candidate modifiable risk factor (smoking, heavy alcohol consumption, or high body mass index [BMI]), and computed the C-statistic, net reclassification improvement (NRI), and integrated discrimination improvement (IDI) of incorporating each factor into the CHA2DS2-VASc. Among 1222 ARIC (mean age: 63.4) and 756 CHS (mean age: 79.1) participants with incident AF, during mean follow-up of 6.9 years and 5.7 years, there were 332 and 335 composite events respectively. Compared with never smokers, current smokers had a higher incidence of the composite endpoint in ARIC [HR: 1.65 (1.21-2.26)] but not in CHS [HR: 1.05 (0.69-1.61)]. In ARIC, the addition of current smoking did not improve risk prediction over and above the CHA2DS2-VASc. No significant associations were observed with alcohol consumption or BMI with CVD outcomes in AF patients from either cohort. Smoking is associated with an increased risk of ischemic stroke or CV death in ARIC, which comprised mostly middle-aged to young-old (65-74 years), but not in CHS, which comprised mostly middle-old or oldest-old (≥75 years) adults with AF. However, addition of smoking to the CHA2DS2-VASc score did not improve risk prediction of these outcomes.

  18. Association of Smoking, Alcohol, and Obesity with Cardiovascular Death and Ischemic Stroke in Atrial Fibrillation: The Atherosclerosis Risk in Communities (ARIC Study and Cardiovascular Health Study (CHS.

    Directory of Open Access Journals (Sweden)

    Younghoon Kwon

    Full Text Available Atrial fibrillation (AF is associated with an increased risk of ischemic stroke and cardiovascular (CV death. Whether modifiable lifestyle risk factors are associated with these CV outcomes in AF is unknown. Among Atherosclerosis Risk in Communities (ARIC study and Cardiovascular Health Study (CHS participants with incident AF, we estimated the risk of composite endpoint of ischemic stroke or CV death associated with candidate modifiable risk factor (smoking, heavy alcohol consumption, or high body mass index [BMI], and computed the C-statistic, net reclassification improvement (NRI, and integrated discrimination improvement (IDI of incorporating each factor into the CHA2DS2-VASc. Among 1222 ARIC (mean age: 63.4 and 756 CHS (mean age: 79.1 participants with incident AF, during mean follow-up of 6.9 years and 5.7 years, there were 332 and 335 composite events respectively. Compared with never smokers, current smokers had a higher incidence of the composite endpoint in ARIC [HR: 1.65 (1.21-2.26] but not in CHS [HR: 1.05 (0.69-1.61]. In ARIC, the addition of current smoking did not improve risk prediction over and above the CHA2DS2-VASc. No significant associations were observed with alcohol consumption or BMI with CVD outcomes in AF patients from either cohort. Smoking is associated with an increased risk of ischemic stroke or CV death in ARIC, which comprised mostly middle-aged to young-old (65-74 years, but not in CHS, which comprised mostly middle-old or oldest-old (≥75 years adults with AF. However, addition of smoking to the CHA2DS2-VASc score did not improve risk prediction of these outcomes.

  19. A grander challenge: the case of how Makerere University College of Health Sciences (MakCHS contributes to health outcomes in Africa

    Directory of Open Access Journals (Sweden)

    Groves Sara

    2011-03-01

    Full Text Available Abstract Background “Grand challenges” in global health have focused on discovery and development of technologies to save lives. The “grander challenge” involves building institutions, systems, capacity and demand to effectively deliver strategies to improve health. In 2008, Makerere University began a radical institutional change to bring together four schools under one College of Health Sciences. This paper’s objective is to demonstrate how its leadership in training, research, and services can improve health in Uganda and internationally, which lies at the core of the College’s vision. Methods A comprehensive needs assessment involved five task forces that identified MakCHS’s contribution to the Ugandan government health priorities. Data were collected through analysis of key documents; systematic review of MakCHS publications and grants; surveys of patients, students and faculty; and key informant interviews of the College’s major stakeholders. Four pilot projects were conducted to demonstrate how the College can translate research into policy and practice, extend integrated outreach community-based education and service, and work with communities and key stakeholders to address their priority health problems. Results MakCHS inputs to the health sector include more than 600 health professionals graduating per year through 23 degree programs, many of whom assume leadership positions. MakCHS contributions to processes include strengthened approaches to engaging communities, standardized clinical care procedures, and evidence-informed policy development. Outputs include the largest number of outpatients and inpatient admissions in Uganda. From 2005-2009, MakCHS also produced 837 peer-reviewed research publications (67% in priority areas. Outcomes include an expanded knowledge pool, and contributions to coverage of health services and healthy behaviors. Impacts include discovery and applications of global significance, such as

  20. Caspofungin Treatment of Aspergillus fumigatus Results in ChsG-Dependent Upregulation of Chitin Synthesis and the Formation of Chitin-Rich Microcolonies.

    Science.gov (United States)

    Walker, Louise A; Lee, Keunsook K; Munro, Carol A; Gow, Neil A R

    2015-10-01

    Treatment of Aspergillus fumigatus with echinocandins such as caspofungin inhibits the synthesis of cell wall β-1,3-glucan, which triggers a compensatory stimulation of chitin synthesis. Activation of chitin synthesis can occur in response to sub-MICs of caspofungin and to CaCl2 and calcofluor white (CFW), agonists of the protein kinase C (PKC), and Ca(2+)-calcineurin signaling pathways. A. fumigatus mutants with the chs gene (encoding chitin synthase) deleted (ΔAfchs) were tested for their response to these agonists to determine the chitin synthase enzymes that were required for the compensatory upregulation of chitin synthesis. Only the ΔAfchsG mutant was hypersensitive to caspofungin, and all other ΔAfchs mutants tested remained capable of increasing their chitin content in response to treatment with CaCl2 and CFW and caspofungin. The resulting increase in cell wall chitin content correlated with reduced susceptibility to caspofungin in the wild type and all ΔAfchs mutants tested, with the exception of the ΔAfchsG mutant, which remained sensitive to caspofungin. In vitro exposure to the chitin synthase inhibitor, nikkomycin Z, along with caspofungin demonstrated synergistic efficacy that was again AfChsG dependent. Dynamic imaging using microfluidic perfusion chambers demonstrated that treatment with sub-MIC caspofungin resulted initially in hyphal tip lysis. However, thickened hyphae emerged that formed aberrant microcolonies in the continued presence of caspofungin. In addition, intrahyphal hyphae were formed in response to echinocandin treatment. These in vitro data demonstrate that A. fumigatus has the potential to survive echinocandin treatment in vivo by AfChsG-dependent upregulation of chitin synthesis. Chitin-rich cells may, therefore, persist in human tissues and act as the focus for breakthrough infections.

  1. Biocompatibility of Schwann Cells with PDLLA/CS/CHS Self-assembled Composite Materials%雪旺细胞与PDLLA/CS/CHS自组装复合材料生物相容性研究

    Institute of Scientific and Technical Information of China (English)

    杨利剑; 梅玉峰; 徐海星

    2011-01-01

    Objective To investigate the biocompatibility of self-cultured Schwann cells with polylactic acid/chondroitin sulfate/chitosan(PDLLA/CS/CHS) composite material with good biodegradability, and evaluate the possibility of the PDLLA/CS/CHS material applied to the repair of peripheral nerve. Methods By using pairs of differential adhesion,Schwann cells were cultured and purified. The growth curve of Schwann cells was drawn,and their phenotypes were examined by using HF staining and immunohistochemical staining. The purified Schwann cells were seeded in the PDLLA/CS/CHS composite materials and the biocompatibility was studied by using MTT method and environmental scanning electron microscopy. Results The growth platform period of Schwann cells was 1 day,the logarithmic period was 5-7 days, and the doubling time was 5 days with the purity of more than 90 %. MTT assay revealed that at 0,2nd,4th day after inoculation.The absorbance(A)values in PDLLA group,CHS group,and PDLLA/CS/CHS group were lower than in control group. At the 7th and lOth day, the A values in PDLLA and CHS groups were also lower than in control group,and those in PDLLA/CS/CHS group were higher than in control group with no significant difference(P>0. 05). But there was significant difference in the A values between PDLLA/CS/CHS group and PDLLA group(P<0. 05). Conclusion PDLLA/CS/CHS composite is biological materials with ideal biocompatibility and good material-cell interface.and facilitates the adhesion,growth and proliferation of Schwann cells.%目的 利用自行培养的雪旺细胞(SCs)与具有良好可生物降解性的高分子聚乳酸/硫酸软骨素/壳聚糖(PDLLA/CS/CHS)复合材料进行生物相容性研究,评价该材料应用于周围神经修复的可能性.方法 利用双差速贴壁法进行SCs的培养与纯化,并观察其生长曲线,苏木精-伊红染色和免疫组织化学染色观察细胞表征;将纯化后的SCs接种在PDLLA/CS/CHS复合材料上进行材料生物

  2. Differenz als Belastungsprobe. Verhandlungen des deutsch-jüdischen Gesprächs in Bernhard Schlinks Erzählung »Die Beschneidung«

    Directory of Open Access Journals (Sweden)

    Mirjam Bitter

    2015-05-01

    Full Text Available Schlinks Erzählung stellt anhand zweier Liebender das Scheitern eines deutsch-jüdischen Gesprächs dar und verhandelt die Frage, wie viele Differenzen eine Beziehung aushält. Die titelgebende Beschneidung tritt dabei nicht nur auf der Handlungsebene auf, wo der Protagonist durch Konversion zum Judentum die Differenz zu seiner jüdischen Freundin zu überwinden hofft. Auch auf metaphorischer Ebene problematisiert die Erzählung die ›Selbstbeschneidung‹ des Protagonisten, der aus Angst vor Tadel von jüdischen Figuren viele seiner Gedanken nicht ausspricht. Als Opfer antideutscher Vorurteile ruft er diverse antisemitische Stereotype auf. Der Beitrag geht der Frage nach, inwiefern die Erzählung damit für solch stereotypes Gedankengut sensibilisiert und subtil gegen einen antisemitischen und revisionistischen Diskurs anschreibt oder inwiefern sich der Text vielmehr in einen solchen Diskurs einschreibt.

  3. Combinatorial analysis of lupulin gland transcription factors from R2R3Myb, bHLH and WDR families indicates a complex regulation of chs_H1 genes essential for prenylflavonoid biosynthesis in hop (Humulus Lupulus L.

    Directory of Open Access Journals (Sweden)

    Matoušek Jaroslav

    2012-02-01

    Full Text Available Abstract Background Lupulin glands of hop produce a specific metabolome including hop bitter acids valuable for the brewing process and prenylflavonoids with promising health-beneficial activities. The detailed analysis of the transcription factor (TF-mediated regulation of the oligofamily of one of the key enzymes, i.e., chalcone synthase CHS_H1 that efficiently catalyzes the production of naringenin chalcone, a direct precursor of prenylflavonoids in hop, constitutes an important part of the dissection of the biosynthetic pathways leading to the accumulation of these compounds. Results Homologues of flavonoid-regulating TFs HlMyb2 (M2, HlbHLH2 (B2 and HlWDR1 (W1 from hop were cloned using a lupulin gland-specific cDNA library from the hop variety Osvald's 72. Using a "combinatorial" transient GUS expression system it was shown that these unique lupulin-gland-associated TFs significantly activated the promoter (P of chs_H1 in ternary combinations of B2, W1 and either M2 or the previously characterized HlMyb3 (M3. The promoter activation was strongly dependent on the Myb-P binding box TCCTACC having a core sequence CCWACC positioned on its 5' end region and it seems that the complexity of the promoter plays an important role. M2B2W1-mediated activation significantly exceeded the strength of expression of native chs_H1 gene driven by the 35S promoter of CaMV, while M3B2W1 resulted in 30% of the 35S:chs_H1 expression level, as quantified by real-time PCR. Another newly cloned hop TF, HlMyb7, containing a transcriptional repressor-like motif pdLNLD/ELxiG/S (PDLNLELRIS, was identified as an efficient inhibitor of chs_H1-activating TFs. Comparative analyses of hop and A. thaliana TFs revealed a complex activation of Pchs_H1 and Pchs4 in combinatorial or independent manners. Conclusions This study on the sequences and functions of various lupulin gland-specific transcription factors provides insight into the complex character of the regulation of the

  4. Tests for fatigue behavior of welded CHS-SHS-concrete filled T-joints%圆管-方管混凝土T型节点疲劳性能试验研究

    Institute of Scientific and Technical Information of China (English)

    童乐为; 杨德磊; Zhao X L

    2013-01-01

    Stress concentration factors(SCF) and fatigue life are two main aspects of studying fatigue behavior of tubular joints based on hot spot stress method. 8 welded CHS-SHS concrete filled T-joints were tested under static loading to determine stress concentration factors at hot spot locations and were also tested under cyclic loading to obtain fatigue life data. The results were compared with those for T-joints made of circular hollow section to square hollow section (CHS-SHS) and no concrete filled. The test results indicated that the CHS-SHS concrete filled T-joints exhibit evident improvements in rigidity distribution and have better anti-fatigue performance than the T-joints made of CHS-SHS and no concrete filled do; S-N curves of hollow section tubular T-joints in the relevant codes are not suitable for fatigue performance analysis of CHS-SHS concrete filled T-joints.%基于热点应力法的管节点疲劳性能研究中,热点应力集中系数和疲劳寿命是描述焊接管结构节点疲劳性能的两个主要因素.对8个支管的圆管主管内填充C50等级混凝土方管节点试件(CT1~CT8)进行热点应力试验以确定其热点应力集中系数(SCF),在此基础上进行疲劳试验以确定其疲劳寿命(N3),将试验结果与具有相同几何尺寸和受力条件的圆管-方管节点试验结果及相关规范进行比较分析.研究表明,支管受轴向荷载下,圆管-方管混凝土节点与对应空钢管节点而言,节点区刚度分布明显改善,前者SCF最大值均小于后者SCF最大值,具有更好的抗疲劳性能;规范中关于空钢管节点疲劳性能的S-N曲线不适用圆管-方管混凝土T型节点的疲劳性能分析.

  5. CHS基因起源初探及其在被子植物中的进化分析%A Preliminary Study on the Origin and Evolution of Chalcone Synthase (CHS) Gene in Angiosperms

    Institute of Scientific and Technical Information of China (English)

    黄金霞; 瞿礼嘉; 杨继; 银好; 顾红雅

    2004-01-01

    利用PCR与TAlL-PCR方法,从半月苔(Lunularia cructata(L.)Dum.ex Lindb)中获得了一段长约l 000 bp的基因片段,它与已知的CHS基因在核苷酸水平上的相似性大于56%,在氨基酸水平上的相似性大于60%,所推断的氨基酸序列中酶反应的4个催化位点与已知晶体结构的紫花苜蓿MCHS2A上的催化位点相同,首次证明了苔类植物中可能存在类CHS基因,将CHS基因的起源时间推到苔藓类植物出现之前.以该序列和两种蕨类植物(Psilotumnudum(L.)Griseb.和Equisetum arvense L.)的CHS序列作为外类群,应用邻接法、最大简约法和最大似然法分别构建了被子植物的CHS的分子系统树.结果表明,大部分科中的CHS分布在不同的分支上,而十字花科、可科和禾本科各自聚成一个单系类群.以邻接树为依据,对茄科、旋花科和菊科的CHS基因进行了相对碱基替换速率的检测,发现这三个科内或科间序列的替换速率不一致.被子植物的CHS基因在基因拷贝数目、碱基替换速率以及重复/丢失事件的发生上都存在较大的差异,这种差异可能与被子植物的生活史、生活环境、花的特性以及对外界的防御系统等的多样性相关.%By using Thermal Asymmetric Interlaced PCR (TAIL-PCR) method, a DNA fragment of about 1 000 bp was amplified and cloned from a liverwort species (Lunularia cruciata (L.) Dum. ex Lindb). The nucleotide sequence of this fragment and its deduced amino acid sequence shared about 56% and 60% identity with those of exon 2 of CHS genes from vascular plants respectively. The four characteristic catalyzing sites of CHS were found conserved in the deduced amino acid sequences of the fragment when compared with other CHS sequences. This is the first report of cloning a CHS-like gene from liverworts,suggesting that the origin of CHS genes may predate liverworts. Using the CHS-like sequence from L.cruciata and CHS sequences from two fern-alien species, Psilotum

  6. Effects of magnetic field perturbations in the ATF torsatron

    Energy Technology Data Exchange (ETDEWEB)

    Colchin, R.J.; England, A.C.; Isler, R.C.; Murakami, M.; Rasmussen, D.A.; Uckan, T.; Wilgen, J.B. [Oak Ridge National Lab., TN (United States); Aceto, S.C.; Zielinski, J.J. [Rensselaer Polytechnic Inst., Troy, NY (United States)

    1993-10-01

    The effects of errors in the magnetic fields of tokamaks on the plasma are quite different from those in stellarators. In tokamaks, field errors can cause disruptive locked modes through the non-linear evolution of tearing modes acting on initially small error-induced islands. Scaling predictions for these effects indicate that the critical relative field error which can be tolerated becomes smaller as the tokamak size becomes larger. In stellarators, the effect is more benign, as field errors appear only to cause increased plasma transport in the vicinity of islands. Great care has been taken to minimize magnetic field errors in the most recent generation of stellarator-type magnetic plasma traps. In the past six years, several new and sensitive techniques have been developed to detect and map field errors. These methods all rely on the detection of electrons injected along magnetic field lines. During the commissioning of ATF, flux surfaces were mapped using the fluorescent screen technique. Field errors were discovered and traced to uncompensated dipoles in the helical current feeds. Prior to elimination of these errors, plasma discharges indicated centrally peaked plasma profiles. After correction of the uncompensated dipoles, flux surfaces were mapped a second time, and the island widths were found to be greatly reduced. Field errors were then deliberately introduced using a set of perturbation coils that had been added to ATF, and electron-beam mapping of the flux surfaces showed that islands several centimeters in width could easily be created by these coils. After elimination of the error fields, the measured plasma temperature and density profiles were much broader. The field-perturbation coils were then used to produce magnetic field asymmetries, and the measured plasma profiles were again shown to narrow as a result of islands.

  7. A Low-temperature Alkaline Protease Producing Marine Psychrophilic Bacterium CHS%产碱性蛋白酶的海洋适冷细菌CHS的初步研究

    Institute of Scientific and Technical Information of China (English)

    陈静; 王淑军; 潘建梅; 许冰; 牛天贵

    2004-01-01

    从连云港海域、港口、远洋捕捞船及鱼市等地采集的海水和各类海鱼、贝类等样品中分离得到一株产碱性蛋白酶的海洋适冷细菌CHS.研究表明,CHS最适生长温度和产酶温度均在15℃左右,0℃下仍可生长;具有一定的耐盐性和嗜盐性,无盐条件下不能生长,在质量分数为5%的NaCl中生长最好;最适生长pH为8.0,最适产酶pH为9.0.

  8. Eletroforese para detecção de hemoglobina fetal em pacientes do ambulatório de hematologia e hemoterapia do Conjunto Hospitalar de Sorocaba (CHS com anemia falciforme e uso de hidroxiuréia

    Directory of Open Access Journals (Sweden)

    Laura Damada Garcia

    2015-10-01

    Full Text Available Introdução: As hemoglobinopatias são distúrbios genéticos que se apresentam por padrão de herança recessiva e incluem a anemia falciforme e as síndromes falcêmicas (hemoglobinopatia SC e a S/β-talassemia. Na anemia falciforme as moléculas de adesão dos eritrócritos, leucócitos e plaquetas estão envolvidas na oclusão vascular. Nas síndromes falcêmicas, o quadro costuma ser mais brando. Pesquisas demonstram que o tratamento com hidroxiuréia (HU promove melhora da qualidade de vida dos pacientes que a utilizam. O mesmo tem se mostrado efetivo por aumentar a expressão de hemoglobina fetal (HbF, diminuindo assim as complicações de vaso-oclusão, hemólise e diminuição da expressão de moléculas de adesão. Objetivos: Nesta pesquisa, utilizamos o teste de eletroforese alcalina (pH 8,6 para o monitoramento de expressão da HbF nos pacientes do Ambulatório de Hematologia e Hemoterapia do CHS portadores de anemia falciforme e nos portadores de síndromes falcêmicas em tratamento com HU. Metodologia: Aplicou-se o teste de eletroforese em acetato de celulose em pH alcalino (8,6 e, posteriormente, as fitas de acetato submetidas ao campo elétrico foram coradas por Ponceau, transparentizadas e fixadas em lâminas de microscopia para análise e registro. Resultados: Foram realizados 100 testes cegos em amostras de sangue provenientes dos pacientes do Ambulatório de Anemia Falciforme do CHS, entre os quais analisamos 28 pacientes com anemia falciforme e 12 com síndromes falcêmicas. Na avaliação eletroforética do uso ou não uso de hidroxiuréia nos grupos investigados, observamos que não houve diferença significativa na expressão de HbF (p=0,3173. Além disso, comparamos os índices hematimétricos dos dois grupos e verificamos que os valores médios de VCM e HCM aumentaram em relação à concentração de HU utilizada, embora não tenham sido analisados estatisticamente.

  9. Electronic, optical properties and chemical bonding in six novel 1111-like chalcogenide fluorides AMChF (A=Sr, Ba; M=Cu, Ag; and Ch=S, Se, Te) from first principles calculations

    Science.gov (United States)

    Bannikov, V. V.; Shein, I. R.; Ivanovskii, A. L.

    2012-12-01

    Employing first-principles band structure calculations, we have examined the electronic, optical properties and the peculiarities of the chemical bonding for six newly synthesized layered quaternary 1111-like chalcogenide fluorides SrAgSF, SrAgSeF, SrAgTeF, BaAgSF, BaAgSeF, and SrCuTeF, which are discussed in comparison with some isostructural 1111-like chalcogenide oxides. We found that all of the studied phases AMChF (A=Sr, Ba; M=Cu, Ag; and Ch=S, Se, Te) are semiconductors for which the fitted “experimental” gaps lie in the interval from 2.23 eV (for SrAgSeF) to 3.07 eV (for SrCuTeF). The near-Fermi states of AMChF are formed exclusively by the valence orbitals of the atoms from the blocks (MCh); thus, these phases belong to the layered materials with “natural multiple quantum wells”. The bonding in these new AMChF phases is described as a high-anisotropic mixture of ionic and covalent contributions, where ionic M-Ch bonds together with covalent M-Ch and Ch-Ch bonds take place inside blocks (MCh), while inside blocks (AF) and between the adjacent blocks (MCh)/(AF) mainly ionic bonds emerge.

  10. Isolation and sequence analysis of chalcone synthase gene BaCHS from Brassica albograbra%芥蓝查尔酮合成酶基因BaCHS的克隆与序列分析

    Institute of Scientific and Technical Information of China (English)

    蒋明; 苗立祥; 胡齐赞; 贺蔡明; 陈珍

    2009-01-01

    查尔酮合成酶基因是类黄酮生物合成途径的关键基因,在植物发育和逆境反应中起着重要作用.根据已发表的查尔酮合成酶基因序列设计全长引物,以芥蓝(Brassica albograbra)叶片基因组DNA和花蕾cDNA为模板,克隆到了芥蓝查尔酮合成酶基因全长序列,命名为BaCHS.该基因DNA全长为1 263 bp,具一个长度为75 bp的内含子,编码区长度为1 188 bp,编码395个氨基酸.序列比对结果表明,BaCHS基因编码的氨基酸序列与其他十字花科植物之间的同源性很高,仅存在个别氨基酸残基的差别.

  11. Finite Element Analysis on In-Plane Effective Length of Compressive Web Members in CHS Truss%圆管桁架受压腹杆平面内计算长度有限元分析

    Institute of Scientific and Technical Information of China (English)

    林彦; 周学军

    2013-01-01

    利用有限元分析方法研究了平面圆管桁架受压腹杆平面内计算长度的主要影响参数以及计算长度随各参数的变化规律.研究结果表明:影响圆管桁架受压腹杆平面内计算长度的主要因素为受压腹杆与弦杆的直径比、壁厚比以及弦杆直径与壁厚的比值,并且随着上述各参数的增大,受压腹杆平面内计算长度系数均呈增大的趋势.最后,根据几十种不同影响参数的有限元计算结果,提出了可供工程应用的圆管桁架受压腹杆平面内计算长度系数的建议值.%In the paper, the main influencing parameters on in-plane effective length for compressive web members in circular hollow section (CHS) truss are studied by using finite element method. The results show that the diameter ratio and wall thickness ratio of compressive web members to chord members,and diameter-to-thickness ratio of chord members have remarkable effects on the in-plane effective length of compressive web members. The results also show that with the increasing of these three parameters, the in-plane effective length factor increases. Based on analytical results of many kinds of models with different dimensions, the suggestive values for in-plane effective length factors of compressive web members are proposed, which can provide reference for engineering design.

  12. Electronic, optical properties and chemical bonding in six novel 1111-like chalcogenide fluorides AMChF (A=Sr, Ba; M=Cu, Ag; and Ch=S, Se, Te) from first principles calculations

    Energy Technology Data Exchange (ETDEWEB)

    Bannikov, V.V.; Shein, I.R. [Institute of Solid State Chemistry, Ural Branch of the Russian Academy of Sciences, 620990 Ekaterinburg (Russian Federation); Ivanovskii, A.L., E-mail: ivanovskii@ihim.uran.ru [Institute of Solid State Chemistry, Ural Branch of the Russian Academy of Sciences, 620990 Ekaterinburg (Russian Federation)

    2012-12-15

    Employing first-principles band structure calculations, we have examined the electronic, optical properties and the peculiarities of the chemical bonding for six newly synthesized layered quaternary 1111-like chalcogenide fluorides SrAgSF, SrAgSeF, SrAgTeF, BaAgSF, BaAgSeF, and SrCuTeF, which are discussed in comparison with some isostructural 1111-like chalcogenide oxides. We found that all of the studied phases AMChF (A=Sr, Ba; M=Cu, Ag; and Ch=S, Se, Te) are semiconductors for which the fitted 'experimental' gaps lie in the interval from 2.23 eV (for SrAgSeF) to 3.07 eV (for SrCuTeF). The near-Fermi states of AMChF are formed exclusively by the valence orbitals of the atoms from the blocks (MCh); thus, these phases belong to the layered materials with 'natural multiple quantum wells'. The bonding in these new AMChF phases is described as a high-anisotropic mixture of ionic and covalent contributions, where ionic M-Ch bonds together with covalent M-Ch and Ch-Ch bonds take place inside blocks (MCh), while inside blocks (AF) and between the adjacent blocks (MCh)/(AF) mainly ionic bonds emerge. - Graphical Abstract: Isoelectronic surface for SrAgSeF and atomic-resolved densities of states for SrAgTeF, and SrCuTeF. Highlights: Black-Right-Pointing-Pointer Very recently six new layered 1111-like chalcogenide fluorides AMChF were synthesized. Black-Right-Pointing-Pointer Electronic, optical properties for AMChF phases were examined from first principles. Black-Right-Pointing-Pointer All these materials are characterized as non-magnetic semiconductors. Black-Right-Pointing-Pointer Bonding is highly anisotropic and includes ionic and covalent contributions. Black-Right-Pointing-Pointer Introduction of magnetic ions in AMChF is proposed for search of novel magnetic materials.

  13. Perfil clínico e laboratorial e sua relação com a adesão ao tratamento de pacientes com Diabetes Mellitus tipo 1 pediátricos atendidos no ambulatorio de endocrinologia do CHS

    Directory of Open Access Journals (Sweden)

    Caroline Rosa Pellicciari

    2014-10-01

    Full Text Available Introdução: Diabetes mellitus (DM refere-se a um grupo de distúrbios metabólicos que compartilham o fenótipo de hiperglicemia. A conduta terapêutica atual no DM tipo 1 inclui a insulina, monitorização e educação em diabetes, incluindo em educação a alimentação, a atividade física e a orientação para os pacientes e suas famílias.Objetivos: avaliação do perfil clínico e laboratorial de crianças e adolescentes com DM1. E, relacionar a adesão ao tratamento com os valores dos exames laboratoriais (Hemoglobina Glicada. Metodologia:análise do prontuário de 56 pacientes (2-17 anos atendidos no ambulatório de endocrinologia pediátrica CHS (Conjunto Hospitalar de Sorocaba, durante o período: 01/08/2013 a 01/07/2014.  Dados como idade, duração da doença, diabetes autoimune ou idiopático, número de aplicações diárias de insulina, valor da hemoglobina glicada, monitorização da glicemia capilar, alimentação, ingestão de doces e refrigerantes e pratica de atividade física foram analisados. Resultados:A idade média foi igual a 10,5 anos. Trinta pacientes estavam em estado de cetoacidose diabética quando foram diagnosticados portadores de DM1; 57,14% dos pacientes apresentaram valor de HbA1c > 9% (mal controle. O grupo de mal controle mostrou maiores idades, sendo composta principalmente por adolescentes (p=0,0133.A ingestão semanal de refrigerante é associada ao grupo de mal controle (p=0,0263. Os demais fatores estudados não mostraram diferença estatística em relação ao mal e bom controle. Conclusões:Apesar de o atendimento ter sido em um centro universitário, um grande percentual de pacientes com DM1 apresentou um controle inadequado da doença, principalmente na faixa etária adolescente. O diagnóstico do DM1 na infância e na adolescência ainda é tardio em nosso meio, com elevada presença de cetoacidose diabética ao diagnóstico.

  14. Prevalência dos riscos cardiovasculares em escolares e adolescentes obesos acompanhados no ambulatório de endocrinologia pediátrica do Conjunto Hospitalar de Sorocaba (CHS

    Directory of Open Access Journals (Sweden)

    Karlla Karollynna de Fátima Gaiotto

    2015-10-01

    Full Text Available Crianças e adolescentes obesos têm alto risco de desenvolver doençascardiovasculares na idade adulta. Assim, a detecção de fatores de risco e educaçãodos pacientes na infância é de extrema importância para o prognóstico, já que nesseperíodo a mudança dos hábitos de vida demonstra grande redução na incidência egravidade de doenças cardíacas. Desta forma, o objetivo do projeto foi avaliarrepercussões metabólicas associadas à obesidade nos pacientes atendidos noambulatório de endocrinologia pediátrica do CHS e seu impacto nos riscoscardiovasculares. Para isto foi realizada a análise do perfil bioquímico,antropométrico e níveis pressóricos de escolares e adolescentes obesos com idadeentre 07 e 18 anos no período de agosto de 2014 a maio de 2015 e calculada aprevalência para cada variável analisada. Dos 49 pacientes, 57,1% nasceram compeso adequado, 46,9% praticam atividade física insuficiente, 42,9% apresentamobesidade na família, 59,1% apresentam parentes obesos com algumacomorbidade, 81,3% se encaixam no percentil acima de 99 para o IMC, 90,55%apresentam circunferência de cintura acima de 71 cm, 44,1% foram consideradoshipertensos, 20,4% apresentaram alterações no perfil glicêmico, 34,7% possuemcolesterol total aumentado, 59,2% estão com HDL inferior ao desejável, 40,9%estão com níveis alterados de LDL, 49% apresentaram valores de VLDL elevado e30,6% apresentaram triglicérides aumentados. A análise dos dados deste estudopermite inferir que o IMC, pressão arterial, circunferência abdominal, colesterol total,HDL colesterol e triglicérides são aqueles cujos valores são mais expressivos, demonstrando as consequências diretas da obesidade no aumento do risco deeventos cardiovasculares futuros.

  15. Proceedings of US-Japan heliotron-stellarator workshop: Volume 3

    Energy Technology Data Exchange (ETDEWEB)

    1987-01-01

    This paper is the third of four volumes on the US-Japan Heliotron-Stellarator Workshop. It contains talks on the following: Heliotron EICRF Heating Experiment; CHS Heating Systems (NBI, ECH, ICH); ICH Program for ATF; ICRF Wave Propagation; the HBQM Heliac Work; configuration studies; compact torsatron studies; low aspect ratio torsatron design; optimized small stellarator designs; configuration studies for ATF; currents in ATF; currents in ATF; computations of 3-D equilibria with islands; magnetic surface mapping studies; magnetic field alignment and mapping on ATF; divertor experiments in IMS; PMI program and wall conditioning for ATF; hard X-ray suppression on ATF; plasma rotation and potential measurement; and status of heavy ion beam probe for ATF.

  16. 猕猴桃花青素合成途径基因AcCHS和AcLDOX的克隆与表达分析%Cloning and Expression of Anthocyanin Pathway Genes,AcCHS and AcLDOX,in Actinidia chinensis

    Institute of Scientific and Technical Information of China (English)

    张亮; 满玉萍; 姜正旺; 王彦昌

    2012-01-01

    Based on the highly conserved sequences of other plants,counterpart sequences of CHS(chalcone synthase,CHS)and LDOX(leucoanthocyanidin dioxygenase,LDOX)of Actinida chinesis,‘Hongyang’were obtained using the special primers. Full-length cDNAs encoding CHS and LDOX were cloned respectively from fruit of‘Hongyang’by RACE(rapid amplification of cDNA ends). The AcCHS was 1501 bp in length,encoding 389 amino acids. AcLDOX was 1381 bp in length and encoded 355 amino acids. Amino acids sequence of AcCHS exhibited over 95% homology with CHS of Abelmoschus manihot,Camellia japonica and Gossypium hirsutum. It also shared 94% and 93% homology with Vitis vinifera and Malus × domestica respectively. AcLDOX showed 94% and 93% identity with Vitis amurensis and Vitis vinifera respectively at amino acids level. The expression of AcCHS and AcLDOX in the inner pericarp of three kiwifruit cultivars with red,green and yellow color was analyzed by Real-time PCR. AcCHS expressed highly in flesh of‘Hongyang’at 65 DAF(days after flower). Expression of the gene in yellow-fleshed‘Jinnong’decreased permanently after flowering. Expression of AcLDOX increased during the early developmental stage in‘Hongyang’,but dropped quickly after 65 DAF. Interestingly,expression of AcLDOX raised markedly in the late developmental stages of green-fleshed‘Jinkui’,which was the highest among three cultivars. AcLDOX in‘Jinnong’also deregulated after flowering.%根据物种水平上蛋白保守序列设计特异引物,扩增获得‘红阳’猕猴桃花青素合成途径中查尔酮合成酶(chalcone synthase,CHS)和无色花青素双加氧酶(leucoanthocyanidin dioxygenase,LDOX)基因的特异片段,用RACE(rapid-amplification of cDNA ends)技术克隆出这两个基因的cDNA全长,长度分别为1501 bp(AcCHS)和1381 bp(AcLDOX),分别编码389个和355个氨基酸。通过比对发现AcCHS与棉花(Gossypium hirsutum

  17. Energy confinement scaling from the international stellarator database

    Energy Technology Data Exchange (ETDEWEB)

    Stroth, U. [Max-Planck-Institut fuer Plasmaphysik, Garching (Germany); Murakami, M.; Dory, R.A.; Yamada, H.; Okamura, S.; Sano, F.; Obiki, T.

    1995-09-01

    An international stellarator database on global energy confinement is presented comprising data from the ATF, CHS and Heliotron E heliotron/torsatrons and the W7-A and W7-AS shearless stellarators. Regression expressions for the energy confinement time are given for the individual devices and the combined dataset. A comparison with tokamak L mode confinement is discussed on the basis of various scaling expressions. In order to make this database available to interested colleagues, the structure of the database and the parameter list are explained in detail. More recent confinement results incorporating data from enhanced confinement regimes such as H mode are reported elsewhere. (author).

  18. Dicty_cDB: CHS392 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Xenopus laevis MGC81993 protein, m... 62 5e-10 BC155581_1( BC155581 |pid:none) Danio rerio scinderin like a,... mRNA... 64 8e-10 BC125898_1( BC125898 |pid:none) Danio rerio scinderin like a, m...C094991 |pid:none) Danio rerio scinderin like a, mRNA... 64 8e-10 AJ719674_1( AJ719674 |pid:none) Gallus gal

  19. Dicty_cDB: CHS265 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available yl-CoA synthetase (fcsA) mRNA, complete cds. 64 1e-09 2 BQ875501 |BQ875501.1 QGI8E20.yg.ab1 QG_ABCDI lettuce salinas...Q870927.1 QGI10G21.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGI10G21, mRNA sequence. 54 0.0

  20. Dicty_cDB: CHS136 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 33 |BQ867133.1 QGC9O01.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC9O01, mRNA sequence. 34 ...7e-04 3 BQ858872 |BQ858872.1 QGC11H15.yg.ab1 QG_ABCDI lettuce salinas Lactuca sat

  1. Dicty_cDB: CHS291 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nce. 48 0.29 1 CO017578 |CO017578.1 EST787960 Coccidioides posadasii saprobic phase cDNA library, greater than 4kb Coccidioides posad...asii cDNA clone CIBBV35 3' end, mRNA sequence. 40 2.7 2 CF818303 |CF818303.1 EST695685 Coccidioides posadas...ii saprobic phase cDNA library, greater than 4kb Coccidioides posadas...ii cDNA clone CIBB387 3' end, mRNA sequence. 40 2.9 2 CO018200 |CO018200.1 EST788582 Coccidioides posadas...ii saprobic phase cDNA library, greater than 4kb Coccidioides posadasii cDNA clone CIBBZ07 3' end,

  2. Dicty_cDB: CHS145 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available NA clone PIMFW90 3' end, mRNA sequence. 48 0.22 1 DT364098 |DT364098.1 JGI_ANNN9278.rev ANNN Pimephales promelas... Whole (L) Pimephales promelas cDNA clone ANNN9278 3', mRNA sequence. 48 0.22... 1 DT347978 |DT347978.1 JGI_CAAX10802.fwd CAAX Pimephales promelas testis 7-8 month adults, males and female...s pooled (L) Pimephales promelas cDNA clone CAAX10802 5', mRNA sequence. 48 0.22 1 DT309975 |DT309975.1 JGI_...CAAX1517.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas

  3. Dicty_cDB: CHS488 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN9278 3', mRNA sequence. 48 0.21 1... DT347978 |DT347978.1 JGI_CAAX10802.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females ...DT309975 |DT309975.1 JGI_CAAX1517.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales prome...669.rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN5669 3', mRNA sequence. 48 0.2...1 1 DT309974 |DT309974.1 JGI_CAAX1517.rev CAAX Pimephales promelas testis 7-8 mon

  4. Dicty_cDB: CHS326 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 46 0.076 2 DT283771 |DT283771.1 JGI_CAAV11164.rev CAAV Pimephales promelas testis 7-8 month adults, males an...d females pooled (H) Pimephales promelas cDNA clone CAAV11164 3', mRNA sequence. ...48 0.19 1 DT275805 |DT275805.1 JGI_CAAV6564.rev CAAV Pimephales promelas testis 7-8 month adults, males and ...females pooled (H) Pimephales promelas cDNA clone CAAV6564 3', mRNA sequence. 48 0.19 1 DT230922 |DT230922.1... JGI_CAAT5104.rev CAAT Pimephales promelas brain 7-8 month adults, males and fema

  5. Dicty_cDB: CHS272 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available |DT364098.1 JGI_ANNN9278.rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN9278 3',... mRNA sequence. 48 0.26 1 DT347978 |DT347978.1 JGI_CAAX10802.fwd CAAX Pimephales promelas testis 7-8 month a...dults, males and females pooled (L) Pimephales promelas cDNA clone CAAX10802 5', ...mRNA sequence. 48 0.26 1 DT309975 |DT309975.1 JGI_CAAX1517.fwd CAAX Pimephales promelas testis 7-8 month adu...lts, males and females pooled (L) Pimephales promelas cDNA clone CAAX1517 5', mRNA sequence. 48 0.26 1 DT102

  6. Dicty_cDB: CHS318 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN9278 3', mRNA sequence. 48 0.21 1... DT347978 |DT347978.1 JGI_CAAX10802.fwd CAAX Pimephales promelas testis 7-8 month... adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX10802 5', mRNA sequence. 48 0.21 1 ...DT309975 |DT309975.1 JGI_CAAX1517.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales prome...669.rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN566

  7. Dicty_cDB: CHS428 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ne PIMFW90 3' end, mRNA sequence. 48 0.20 1 DT364098 |DT364098.1 JGI_ANNN9278.rev ANNN Pimephales promelas Whole (L) Pimephales prome...47978 |DT347978.1 JGI_CAAX10802.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promela...17.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas...las testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA ...clone CAAX1517 3', mRNA sequence. 48 0.20 1 DT102344 |DT102344.1 JGI_ANNN5669.rev ANNN Pimephales promelas Whole (L) Pimephales prome

  8. Dicty_cDB: CHS483 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ey sequence. 38 0.14 2 AI484136 |AI484136.1 EST248943 tomato resistant, Cornell Lycopersicon esculentum cDNA...490786.1 EST241494 tomato shoot, Cornell Lycopersicon esculentum cDNA clone cLEB3H9 similar to cinnamic acid...34.1 EST255034 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER8N1, mRNA sequence. 38 0.15

  9. Dicty_cDB: CHS321 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available _KB Zea mays genomic clone ZMMBTa391I09,genomic survey sequence. 38 1e-04 3 CK269381 |CK269381.1 EST715459 potato abiotic...1023C12 5', mRNA sequence. 32 0.043 3 CK278044 |CK278044.1 EST724122 potato abiotic stress cDNA library Sola...ce. 46 0.050 2 CK276419 |CK276419.1 EST722497 potato abiotic stress cDNA library Solanum tuberosum cDNA clon...9380.1 EST715458 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POACM48 5' end, mRNA sequen...num tuberosum cDNA clone POAE360 3' end, mRNA sequence. 46 0.049 2 CK269380 |CK26

  10. Dicty_cDB: CHS115 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available rium johnsoniae UW101... 33 3.5 AY524943_1( AY524943 |pid:none) Rhampholeon temporalis isolate 3 r... 32 7.5... DQ385413_1( DQ385413 |pid:none) Phrynosoma modestum recombination ... 32 7.5 AY524942_1( AY524942 |pid:none) Rhampholeon

  11. Dicty_cDB: CHS177 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ases Associated with Cell Cycle. 36 0.020 4 BD452239 |BD452239.1 Diagnosis of Diseases... AX344239 |AX344239.1 Sequence 86 from Patent WO0200926. 36 0.020 4 BD460317 |BD460317.1 Diagnosis of Dise

  12. Dicty_cDB: CHS239 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available neering clone MHPN192d01. 42 9.9 1 AZ781723 |AZ781723.1 2M0021H18F Mouse 10kb plasm...end partial sequence. 42 9.9 1 CR029411 |CR029411.1 Forward strand read from insert in 5'HPRT insertion targeting and chromosome engi

  13. Dicty_cDB: CHS280 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available gene, partial cds. 28 0.11 4 AF134680 |AF134680.1 Plasmodium falciparum strain SEN23 from Senegal CG2 omega ...1 Plasmodium falciparum strain SEN22 from Senegal CG2 omega repeat (cg2) gene, partial cds. 28 0.14 4 AF1346

  14. Dicty_cDB: CHS385 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available QFCTAHF*k*epiyqfmfh fnnq*hwlqilyi*mvvnwlflvivfmi*piisilvlfnvlivnsylxmqshvhyhppqt niy*ik*cl*illllvkvvvdmlfpi*...mvvnwlflvivfmi*piisilvlfnvlivnsylxmqshvhyhppqt niy*ik*cl*illllvkvvvdmlfpi*lhgfnst

  15. Dicty_cDB: CHS420 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available cloneQGC21B24, mRNA sequence. 80 5e-12 2 BH165696 |BH165696.1 ENTTP28TR Entamoeba histolytica Sheared DNA En...tamoeba histolytica genomic, DNA sequence. 68 3e-07 1 BH164508 |BH164508.1 ENTTD23TF Entamoeba histolytica Shear...3900.1 ENTSJ80TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence. 68 3e-07 1 B...H163033 |BH163033.1 ENTRP52TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.... 68 3e-07 1 BH161826 |BH161826.1 ENTRN55TF Entamoeba histolytica Sheared DNA Enta

  16. Dicty_cDB: CHS442 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available s producing significant alignments: (bits) Value N BH148791 |BH148791.1 ENTPI47TR Entamoeba histolytica Shear...ed DNA Entamoeba histolyticagenomic, genomic survey sequence. 48 0.27 1 BH133986 |BH133986.1 ENTOC90TR Entamoeba histolytica Shear...7 1 AZ679166 |AZ679166.1 ENTKM96TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequ...ence. 48 0.27 1 AZ531057 |AZ531057.1 ENTBC39TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genom...ic, DNA sequence. 48 0.27 1 AZ531674 |AZ531674.1 ENTBT90TR Entamoeba histolytica Shear

  17. Dicty_cDB: CHS180 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available era coleoptrata voucher Scol elongation factor 2 mRNA, partial cds. 66 2e-21 3 BI863585 |BI863585.1 kx46a06.y1 Parastrongyloides... trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar ...|BI863575.1 kx45h07.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichos...23P22 3', mRNA sequence. 74 2e-18 2 BI451383 |BI451383.1 kx23e09.y1 Parastrongyloides trichosuri FL pAMP1 v1... Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL

  18. Dicty_cDB: CHS289 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available AATAATAATAATAATAAA AAATAAAGTTATNATAAAT sequence update 2002.10.25 Translated Amino Acid sequence gllei*qfk*lik**hyi*n*illqlynn...iiiiiknkvn**iylnk*kk--- ---ksnnsns*snsnitykikyyynytiifikknnnnnnnnkk*syxk Frame B: gllei*qfk*lik**hyi*n*illqlynn...itykikyyynytiifikknnnnnnnnkk*s*linifk*ikkk--- ---i*qfk*lik**hyi*n*illqlynnfykkk**

  19. Dicty_cDB: CHS232 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available YENSCYXNSSNNQVVEVSNKCFNLDGFIKI TTPTEX--- ---FXMSTQIMKCRFNAPRSSMXCKGSP**mfikmptwp*i*s*sxw*rmcvrs...hrpppp evcslrcppkheckfddhgkkccvkihcdevcdldcgrgfeckirhdgskccvrserphp pqhekcnkrcppgheckvdqhgkecc...kyqinasilmdllk lqlqps--- ---FXMSTQIMKCRFNAPRSSMXCKGSP**mfikmptwp*i*s*sxw*rmcvrshrpppp evcslrcppkheckfddhgkkc...cvkihcdevcdldcgrgfeckirhdgskccvrserphp pqhekcnkrcppgheckvdqhgkeccvvahrpppkcslrcpprhecrvnhfgeeccvkvh hdkcslrc

  20. Dicty_cDB: CHS164 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 2_A029 Roots minus micronutrients Pinus taeda cDNA clone RTMNUT1_37_B06_A029 5', mRNA sequence. 54 2e-11 4 C...icon esculentum cDNA clone cLEG49G24 5' end, mRNA sequence. 58 7e-12 2 DR181209 |DR181209.1 RTMNUT1_37_B06.g

  1. Dicty_cDB: CHS268 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ACATTTNAATAAAATTNTTATTAC ATTTATAA sequence update 2002.10.25 Translated Amino Acid sequence pniih*y*infillhv...hhk slvrenlvkl*sxf*ekqxkilmkleileikytfx*nxyyiy Frame C: pniih*y*infillhvi***ik*nkik*nkiki*iknkknkek*nsqhtnik

  2. Dicty_cDB: CHS346 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ry Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mR...NA sequence. 34 4e-05 3 CK808739 |CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoo...sgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.

  3. Dicty_cDB: CHS144 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available lone: ola1-242I19.F, genomic survey sequence. 54 0.005 1 DE092915 |DE092915.1 Oryzias latipes DNA, clone: ola1-209J09.R, genomic surv...ipes DNA, clone: ola1-188M19.F, genomic survey sequence. 54 0.005 1 DE078411 |DE078411.1 Oryzias latipes DNA..., clone: ola1-200D13.F, genomic survey sequence. 54 0.005 1 DE075571 |DE075571.1 Oryzias latipes DNA, clone:... ola1-178O04.F, genomic survey sequence. 54 0.005 1 DE057397 |DE057397.1 Oryzias latipes DNA, clone: ola1-099G20.R, genomic survey...DNA, clone: ola1-164C24.R, genomic survey sequence. 54 0.005 1 AC027286 |AC027286.14 Homo sapiens chromosome

  4. Dicty_cDB: CHS337 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Grid AA Zea mays genomic, genomic survey sequence. 44 1.5 1 CG711657 |CG711657.1 1119022C08.y1 1119 - Rescu...eMu Grid AA Zea mays genomic, genomic survey sequence. 44 1.5 1 BZ158667 |BZ15866...19.1 Danio rerio genomic clone DKey-7L12, genomic survey sequence. 44 1.5 1 CW928277 |CW928277.1 EDCBQ46TF A...omic clone EDCBQ46, genomic survey sequence. 44 1.5 1 CW918360 |CW918360.1 EDCA491TF A. castellanii, 6-8 kb ...library from total genomic DNA Acanthamoeba castellanii genomic clone EDCA491, genomic survey sequence. 44 1

  5. Dicty_cDB: CHS185 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available |pid:none) Mus musculus sucrase-isomaltase mR... 101 5e-20 AB000967_1( AB000967 |...L25926_1( L25926 |pid:none) Rat sucrase-isomaltase (SI) mRNA, comp... 97 1e-18 ( P23739 ) RecName: Full=Sucrase-isomalt...e-glucoamylase, intestinal; Include... 93 2e-18 BC115034_1( BC115034 |pid:none) Homo sapiens sucrase-isomalt...ase (a... 94 7e-18 ( P14410 ) RecName: Full=Sucrase-isomaltase, intestinal; Contains:... 94 7e-18 BC116452_1...( BC116452 |pid:none) Homo sapiens sucrase-isomaltase (a... 94 9e-18 protein upda

  6. Dicty_cDB: CHS126 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available SJS Schistosoma japonicum cDNA, mRNA sequence. 56 8e-04 1 CV682907 |CV682907.1 service...43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence. 56 8e-04 1 CV682854 |CV682854.1 service

  7. Dicty_cDB: CHS489 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available mRNA sequence. 56 8e-04 1 CV682907 |CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS ...Schistosoma japonicum cDNA, mRNA sequence. 56 8e-04 1 CV682854 |CV682854.1 service30_G09_sjs2-14-10C09-T3 SJ

  8. Dicty_cDB: CHS251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ibrary D Homo sapiens genomic clone Plate=3113 Col=14 Row=F, genomic survey sequence. 44 0.055 2 DT661445 |DT661445.1 est_e_glacial...is75 RWS Eubalaena glacialis cDNA, mRNA sequence. 46 0.71 1 AC116986 |AC116986.2 Dict

  9. Sensor Based Effective Monitoring of Coal Handling System (CHS

    Directory of Open Access Journals (Sweden)

    Kuttalakkani.M

    2013-06-01

    Full Text Available Coal level detection is an important aspect to assess the performance of a coal-fired power plant. Coal has to be transported, via a coal handling system. The fuel in a coal-fired power plant is stored in silos, bunkers or stock piles. Coal is stored in silos in a small plant, Bunkers for handling a day’s operation and Stock piling methods for large plants. So, fuel handling had to done efficiently. To accurately sense the coal height, Real-time feedback is deployed within the bunker or stock pile. The real time range information is then fedback to the control system. Of the different types of ranging sensors, radar based system is used. Also a real-time temperature monitoring system is developed to protect the coal. The range and temperature data from sensors are sent to the main system through GSM modem by means of SMS. The range information is used to start the conveyor belt to draw the coal from coal yard. If the temperature exceeds the limit, the SMS will be sent through the software or it will call the respective person to monitor the process. A fire sensor is also used to extinguish the fire by initiating the water spraying system. A PIC Microcontroller is interfaced all the sensors for effective handling of thermal power plant.

  10. 78 FR 37537 - CHS Inc., Federal Express Corporation, GROWMARK, Inc., HWRT Oil Company LLC, MFA Oil Company...

    Science.gov (United States)

    2013-06-21

    ..., MFA Oil Company, Southwest Airline Co., United Airlines, Inc., UPS Fuel Services, Inc. v. Enterprise... Express Corporation; GROWMARK, Inc.; HWRT Oil Company LLC; MFA Oil Company; Southwest Airline Co.;...

  11. NOAA Office for Coastal Management Coastal Inundation Digital Elevation Model: National Weather Service Forecast Office - Charleston (CHS)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data were created as part of the National Oceanic and Atmospheric Administration Office for Coastal Management's efforts to create an online mapping viewer...

  12. 搭载CHS混合动力系统的城市客车仿真分析%Simulation Analysis on City Bus with CHS Hybrid System

    Institute of Scientific and Technical Information of China (English)

    万辅君; 王伟; 史广奎; 王仁广

    2015-01-01

    在解析CHS混合动力系统的基础上,重点介绍装配CHS混合动力系统的某客车整车控制策略模型开发和整车仿真模型搭建以及仿真结果分析.通过仿真分析,可以清楚了解所开发的整车搭载深度混合动力总成的具体性能状况.

  13. Correlação entre etiologia e a localização das estenoses de uretra - estudo retrospectivo em pacientes atendidos no CHS/PUC-SP

    Directory of Open Access Journals (Sweden)

    Marcelo Cabral Lamy de Miranda

    2008-07-01

    Full Text Available RESUMO Objetivo: análise retrospectiva dos pacientes que estão no programa de dilatação de uretra do Conjunto Hospitalar de Sorocaba, levando em consideração as principais localizações de estenose de uretra relacionada com suas causas específicas e prevalências, no período de fevereiro de 2002 a abril de 2005. Sujeitos e métodos: este trabalho foi realizado mediante estudo retrospectivo dos prontuários médicos e fichas de cadastramento do programa de dilatação de uretra de 115 pacientes registrados no período de fevereiro de 2002 a abril de 2005. Resultados: neste estudo, os pacientes com estenose de uretra tiveram como média de idade 57,5 anos e 3,33% eram do sexo feminino. Nos pacientes do sexo masculino foram encontrados 49,17% das lesões na uretra anterior, enquanto que 47,5% na uretra posterior. As principais localidades de estenose notificadas foram: meato, com 31 casos (25,8%; uretra prostática, com 23 casos (19,1%; colo, com 22 casos (18,33%; fossa navicular, com 21 casos (17,5%; uretra bulbar, com 13 casos (10,8%; uretra peniana, com 6 casos (5%; uretra feminina, com 4 casos (3,33%.

  14. Stress Concentration Factors at Saddle and Crown Positions on the Central Brace of Two-Planar Welded CHS DKT-connections

    Institute of Scientific and Technical Information of China (English)

    Hamid Ahmadi; Mohammad Ali Lotfollahi-Yaghin; Mohammad H. Aminfar

    2012-01-01

    A set of parametric stress analyses was carried out for two-planar tubular DKT-joints under different axial loading conditions.The analysis results were used to present general remarks on the effects of the geometrical parameters on stress concentration factors (SCFs) at the inner saddle,outer saddle,and crown positions on the central brace.Based on results of finite element (FE) analysis and through nonlinear regression analysis,a new set of SCF parametric equations was established for fatigue design purposes.An assessment study of equations was conducted against the experimental data and original SCF database.The satisfaction of acceptance criteria proposed by the UK Department of Energy (UK DoE) was also checked.Results of parametric study showed that highly remarkable differences exist between the SCF values in a multi-planar DKT-joint and the corresponding SCFs in an equivalent uni-planar KT-joint having the same geometrical properties.It can be clearly concluded from this observation that using the equations proposed for uni-planar KT-connections to compute the SCFs in multi-planar DKT-joints will lead to either considerably under-predicting or over-predicting results.Hence,it is necessary to develop SCF formulae specially designed for multi-planar DKT-joints.Good results of equation assessment according to UK DoE acceptance criteria,high values of correlation coefficients,and the satisfactory agreement between the predictions of the proposed equations and the experimental data guarantee the accuracy of the equations.Therefore,the developed equations can be reliably used for fatigue design of offshore structures.

  15. Main: BOX1PVCHS15 [PLACE

    Lifescience Database Archive (English)

    Full Text Available BOX1PVCHS15 S000208 11-May-2006 (last modified) kehi Box 1 of bean (P.v.) chs15 pro...moter; one of SBF-1 binding sites in chs15 promoter; Located at -318 to -305; Involved in organ-specific exp...e Villain et al. (1996); Box 1; chs; chs15; CHS; SBF-1; silencer; organ-specific; Gt; GT-1; GT; bean (Phaseolus vulgaris) TAAAAGTTAAAAAC ...

  16. Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in flavonoid biosynthetic pathway.

    Directory of Open Access Journals (Sweden)

    Wei Sun

    Full Text Available Chalcone synthase (CHS catalyzes the first committed step in the flavonoid biosynthetic pathway. In this study, the cDNA (FhCHS1 encoding CHS from Freesia hybrida was successfully isolated and analyzed. Multiple sequence alignments showed that both the conserved CHS active site residues and CHS signature sequence were found in the deduced amino acid sequence of FhCHS1. Meanwhile, crystallographic analysis revealed that protein structure of FhCHS1 is highly similar to that of alfalfa CHS2, and the biochemical analysis results indicated that it has an enzymatic role in naringenin biosynthesis. Moreover, quantitative real-time PCR was performed to detect the transcript levels of FhCHS1 in flowers and different tissues, and patterns of FhCHS1 expression in flowers showed significant correlation to the accumulation patterns of anthocyanin during flower development. To further characterize the functionality of FhCHS1, its ectopic expression in Arabidopsis thaliana tt4 mutants and Petunia hybrida was performed. The results showed that overexpression of FhCHS1 in tt4 mutants fully restored the pigmentation phenotype of the seed coats, cotyledons and hypocotyls, while transgenic petunia expressing FhCHS1 showed flower color alteration from white to pink. In summary, these results suggest that FhCHS1 plays an essential role in the biosynthesis of flavonoid in Freesia hybrida and may be used to modify the components of flavonoids in other plants.

  17. Physical mechanism determining the radial electric field and its radial structure in a toroidal plasma

    Energy Technology Data Exchange (ETDEWEB)

    Ida, Katsumi; Miura, Yukitoshi; Itoh, Sanae [and others

    1994-10-01

    Radial structures of plasma rotation and radial electric field are experimentally studied in tokamak, heliotron/torsatron and stellarator devices. The perpendicular and parallel viscosities are measured. The parallel viscosity, which is dominant in determining the toroidal velocity in heliotron/torsatron and stellarator devices, is found to be neoclassical. On the other hand, the perpendicular viscosity, which is dominant in dictating the toroidal rotation in tokamaks, is anomalous. Even without external momentum input, both a plasma rotation and a radial electric field exist in tokamaks and heliotrons/torsatrons. The observed profiles of the radial electric field do not agree with the theoretical prediction based on neoclassical transport. This is mainly due to the existence of anomalous perpendicular viscosity. The shear of the radial electric field improves particle and heat transport both in bulk and edge plasma regimes of tokamaks. (author) 95 refs.

  18. One type of chalcone synthase gene expressed during embryogenesis regulates the flavonoid accumulation in citrus cell cultures.

    Science.gov (United States)

    Moriguchi, T; Kita, M; Tomono, Y; EndoInagaki, T; Omura, M

    1999-06-01

    To elucidate the relationship between the expression of chalcone synthase (CHS) genes and the production of flavonoid in citrus cell cultures, two cDNA clones encoding CHS were isolated (CitCHS1 and CitCHS2) from the citrus. The accumulation of CitCHS2 mRNA was notably induced by embryogenesis but CitCHS1 mRNA was not. There was no detectable accumulation of flavonoid in the undifferentiated calli, but flavonoid accumulated after the morphological changes to embryoids. These results indicate that two CHS genes differentially expressed during citrus somatic embryogenesis and CitCHS2 may regulate the accumulation of flavonoid in citrus cell cultures.

  19. Eletroforese para detecção de hemoglobina fetal em pacientes do ambulatório de hematologia e hemoterapia do Conjunto Hospitalar de Sorocaba (CHS) com anemia falciforme e uso de hidroxiuréia

    OpenAIRE

    2015-01-01

    Introdução: As hemoglobinopatias são distúrbios genéticos que se apresentam por padrão de herança recessiva e incluem a anemia falciforme e as síndromes falcêmicas (hemoglobinopatia SC e a S/β-talassemia). Na anemia falciforme as moléculas de adesão dos eritrócritos, leucócitos e plaquetas estão envolvidas na oclusão vascular. Nas síndromes falcêmicas, o quadro costuma ser mais brando. Pesquisas demonstram que o tratamento com hidroxiuréia (HU) promove melhora da qualidade de vida dos pacient...

  20. Avaliação hematológica dos pacientes diagnosticados com aplasia medular do ambulatório de hematologia e hemoterapia do Conjunto Hospitalar de Sorocaba (CHS

    Directory of Open Access Journals (Sweden)

    Ivan Fernandes Filho

    2014-10-01

    Full Text Available Introdução: Aplasia Medular (AM é um distúrbio de produção de células hematopoiéticas decorrentes da perda de tecido medular caracterizado clinicamente por astenia, petéquias, dispneia, equimoses, cefaleia e hemorragias. Objetivos: avaliar os prontuários dos pacientes com AM através da investigação do perfil clínico de cada paciente, as possíveis etiologias da doença, tratamentos realizados e sua evolução. Métodos: analisamos os prontuários de 11 pacientes diagnosticados com AM no momento do diagnóstico e da última consulta, recolhendo informações quanto à etiologia, aos achados laboratoriais e à evolução dos pacientes em cada tratamento. Resultados: A proporção entre homens e mulheres era 63/77. A idade média foi de 31,36 anos. Houve VCM (Máximo=122,64 fL; Mínimo=80,5 fL, Ht (Máximo=31%; Mínimo=6%, leucócitos (Máximo= 7640/μL; Mínimo=800/μL, segmentados (Máximo=3590/μL; Mínimo=161/μL, média de 72401,8 plaquetas/mm3 e mediana de 16000 plaquetas /mm3. 45% dos pacientes foram submetidos ao transplante de medula óssea, sendo que todos se encontram, no momento, vivos e sem a doença. No tratamento com ciclosporina A 300mg+Prednisona 80mg (20% dos pacientes, 50% dos pacientes estão vivos e sem a doença. Conclusão: As etiologias e classificação da doença mostraram que a maioria dos pacientes apresentou AM idiopática em sua forma grave com evolução hematológica satisfatória durante o tratamento.

  1. Infantile Hemophagocytic Lymphohistiocytosis in a Case of Chediak-Higashi Syndrome Caused by a Mutation in the LYST/CHS1 Gene Presenting With Delayed Umbilical Cord Detachment and Diarrhea

    DEFF Research Database (Denmark)

    Nielsen, Christian; Agergaard, Charlotte N; Jakobsen, Marianne A;

    2015-01-01

    perforin content in CD8 T cells seems to correlate to the immune activation state of the patient with 82% and 8% perforin-containing CD8 T cells at active and nonactive hemophagocytic lymphohistiocytosis (HLH) disease, respectively. HLH was confirmed by hemophagocytosis in bone marrow and absent natural...... in this severe phenotype of Chediak-Higashi syndrome was probably induced by rotaviral infection. Interestingly, the intracellular perforin content in CD8 T cells seems to correlate to the immune activation state of the patient. Late separation of the umbilical cord in concordance with clinical symptoms should...

  2. Molecular cloning and expression profiling of a chalcone synthase gene from Lamiophlomis rotata

    Indian Academy of Sciences (India)

    Qiao Feng; Geng Gui-Gong; Zeng Yang; Xie Hui-Chun; Jin Lan; Shang Jun; Chen Zhi

    2015-06-01

    Lamiophlomis rotata is a renowned Chinese medicinal plant. Chalcone synthase (CHS) is important in flavonoid and isoflavonoid biosynthesis, catalysing the formation of naringenin chalcone in plants. A full-length cDNA encoding the CHS gene was cloned from L. rotata based on the highly conserved CHS gene sequences of Labiatae plants. A blast search showed its homology (named LrCHS) with other CHS genes of Labiate plants. The full-length genomic DNA of LrCHS was 2026 bp with one intron of 651 bp, two exons of 178 bp and 998 bp, flanked by a 73 bp $5'$-UTR and a 126 bp $3'$-UTR. The cDNA sequence of the LrCHS gene had an 1176 bp open reading frame encoding a 391 amino acid protein of 42,798 Da. The CHS protein predicted from L. rotata showed 79–86% identity with CHS of other plant species. We conducted a phylogenetic analysis of nine families containing 48 plants and L. rotata based on the full amino acid sequences of CHS proteins. Consequently, LrCHS was located in the Labiatae branch. Additionally, we examined LrCHS gene expression patterns in different tissues by quantitative real-time PCR with specific primers. The expression analysis showed preferential expression of LrCHS in flowers and leaves during the flowering stage. Total flavonoid content and CHS gene expression exhibited similar patterns during L. rotata organ development. In agreement with its function as an elicitor-responsive gene, LrCHS expression was coordinated by methyl jasmonate and UV light, and induced between 6 and 18 h. These results provide a molecular basis for additional functional studies of LrCHS in L. rotata.

  3. Main: BOX2PVCHS15 [PLACE

    Lifescience Database Archive (English)

    Full Text Available BOX2PVCHS15 S000209 11-May-2006 (last modified) kehi Box 2 of bean (P.v.) chs15 pro...moter; SBF-1 binding site; For a compilation of related GT elements and factors, see Villain et al. (1996); Box 2; chs; chs1

  4. Main: BOX3PVCHS15 [PLACE

    Lifescience Database Archive (English)

    Full Text Available BOX3PVCHS15 S000210 11-May-2006 (last modified) kehi Box 3 of bean (P.v.) chs15 pro...moter; SBF-1 binding site; For a compilation of related GT elements and factors, see Villain et al. (1996); Box 3; chs; chs1

  5. Overvej cannabinoid hyperemesis-syndrom ved recidiverende opkastninger

    DEFF Research Database (Denmark)

    Nordholm-Carstensen, Andreas

    2014-01-01

    Cannabinoid hyperemesis syndrome (CHS) is characterised by unrelenting nausea, recurrent vomiting, abdominal pain and compulsive, hot bathing behaviour. The symptoms contrast the traditional effects associated with cannabis use. We report a "textbook example" of a 26-year-old man with CHS. CHS...

  6. AcEST: DK961727 [AcEST

    Lifescience Database Archive (English)

    Full Text Available CHS P... 211 1e-56 sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS... 215 2e-56 sp|P49440|C...R+ Sbjct: 143 PGADYQMTKLLGLRPSVKRV 162 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS PE=

  7. AcEST: DK955270 [AcEST

    Lifescience Database Archive (English)

    Full Text Available hase 1 OS=Glycine max GN=CHS1 ... 291 1e-78 sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS...UELO Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 SV=1 Length = 389 Score = 291 bits (744), Expect = 2e-

  8. Molecular cloning of allelopathy related genes and their relation to HHO in Eupatorium adenophorum.

    Science.gov (United States)

    Guo, Huiming; Pei, Xixiang; Wan, Fanghao; Cheng, Hongmei

    2011-10-01

    In this study, conserved sequence regions of HMGR, DXR, and CHS (encoding 3-hydroxy-3-methylglutaryl-CoA reductase, 1-deoxyxylulose-5-phosphate reductoisomerase and chalcone synthase, respectively) were amplified by reverse transcriptase (RT)-PCR from Eupatorium adenophorum. Quantitative real-time PCR showed that the expression of CHS was related to the level of HHO, an allelochemical isolated from E. adenophorum. Semi-quantitative RT-PCR showed that there was no significant difference in expression of genes among three different tissues, except for CHS. Southern blotting indicated that at least three CHS genes are present in the E. adenophorum genome. A full-length cDNA from CHS genes (named EaCHS1, GenBank ID: FJ913888) was cloned. The 1,455 bp cDNA contained an open reading frame (1,206 bp) encoding a protein of 401 amino acids. Preliminary bioinformatics analysis of EaCHS1 revealed that EaCHS1 was a member of CHS family, the subcellular localization predicted that EaCHS1 was a cytoplasmic protein. To the best of our knowledge, this is the first report of conserved sequences of these genes and of a full-length EaCHS1 gene in E. adenophorum. The results indicated that CHS gene is related to allelopathy of E. adenophorum.

  9. A case of multiple system atrophy: onset with the cold hands sign

    Institute of Scientific and Technical Information of China (English)

    WANG Zhen-fu; WANG Qiong; WU Wei-ping

    2011-01-01

    @@ To the Editor: The cold hands sign (CHS) presents a distinct feature in some multiple system atrophy (MSA) patients, but it is receiving little consideration.To our knowledge, there are few reported cases of MSA with onset of CHS.We reported here a case of MSA in a patient with onset of a typical CHS.

  10. Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis (Meyen).

    Science.gov (United States)

    Zhang, Jianzhen; Liu, Xiaojian; Zhang, Jianqin; Li, Daqi; Sun, Yi; Guo, Yaping; Ma, Enbo; Zhu, Kun Yan

    2010-11-01

    Chitin synthases are crucial enzymes responsible for chitin biosynthesis in fungi, nematodes and arthropods. We characterized two alternative splicing-derived variants of chitin synthase 1 gene (LmCHS1) from the oriental migratory locust, Locusta migratoria manilensis (Meyen). Each cDNA of the two variants (LmCHS1A and LmCHS1B) consists of 5116 nucleotides that include a 4728-nucleotide open reading frame (ORF) encoding 1576 amino acid residues, and 67- and 321-bp non-coding regions at the 5'- and 3'-ends of the cDNA, respectively. The two variants differ only in one exon consisting of 177 nucleotides that encode 59 amino acid residues. The amino acid sequences within this alternative splicing region are 75% identical between the two variants. Both variants were expressed in all the developmental stages. However, LmCHS1A was predominately expressed in the integument whereas LmCHS1B was mainly expressed in the trachea. Our RNAi-based gene silencing study resulted in a dramatic reduction in the levels of the corresponding mRNA in the locust nymphs injected with dsRNA of LmCHS1, or either of its two variants, LmCHS1A and LmCHS1B. Consequentially, 95, 88 and 51% of mortalities were observed in the locusts injected with the LmCHS1, LmCHS1A and LmCHS1B dsRNA, respectively. The phenotypes resulted from the injection of LmCHS1A dsRNA were similar to those from the injection of LmCHS1 dsRNA, whereas the locusts injected with LmCHS1B dsRNA exhibited crimpled cuticle phenotype. Our results suggest that both variants of chitin synthase 1 are essential for insect growth and development.

  11. Endogenous, tissue-specific short interfering RNAs silence the chalcone synthase gene family in glycine max seed coats.

    Science.gov (United States)

    Tuteja, Jigyasa H; Zabala, Gracia; Varala, Kranthi; Hudson, Matthew; Vodkin, Lila O

    2009-10-01

    Two dominant alleles of the I locus in Glycine max silence nine chalcone synthase (CHS) genes to inhibit function of the flavonoid pathway in the seed coat. We describe here the intricacies of this naturally occurring silencing mechanism based on results from small RNA gel blots and high-throughput sequencing of small RNA populations. The two dominant alleles of the I locus encompass a 27-kb region containing two perfectly repeated and inverted clusters of three chalcone synthase genes (CHS1, CHS3, and CHS4). This structure silences the expression of all CHS genes, including CHS7 and CHS8, located on other chromosomes. The CHS short interfering RNAs (siRNAs) sequenced support a mechanism by which RNAs transcribed from the CHS inverted repeat form aberrant double-stranded RNAs that become substrates for dicer-like ribonuclease. The resulting primary siRNAs become guides that target the mRNAs of the nonlinked, highly expressed CHS7 and CHS8 genes, followed by subsequent amplification of CHS7 and CHS8 secondary siRNAs by RNA-dependent RNA polymerase. Most remarkably, this silencing mechanism occurs only in one tissue, the seed coat, as shown by the lack of CHS siRNAs in cotyledons and vegetative tissues. Thus, production of the trigger double-stranded RNA that initiates the process occurs in a specific tissue and represents an example of naturally occurring inhibition of a metabolic pathway by siRNAs in one tissue while allowing expression of the pathway and synthesis of valuable secondary metabolites in all other organs/tissues of the plant.

  12. Down-regulation of a chitin synthase a gene by RNA interference enhances pathogenicity of Beauveria bassiana ANU1 against Spodoptera exigua (HÜBNER).

    Science.gov (United States)

    Lee, Jung-Bok; Kim, Hyun Soo; Park, Youngjin

    2017-02-01

    Chitin synthase (CHS) is an important enzymatic component, which is required for chitin formation in the cuticles and cuticular linings of other tissues in insects. CHSs have been divided into two classes, classes A and B, based on their amino acid sequence similarities and functions. Class A CHS (CHS-A) is specifically expressed in the epidermis and related ectodermal cells such as tracheal cells, while class B CHS (CHS-B) is expressed in gut epithelial cells that produce peritrophic matrices. In this study, we cloned the CHS-A gene from the beet armyworm, Spodoptera exigua (SeCHS-A). The SeCHS-A contains an open reading frame of 4,698 nucleotides, encoding a protein of 1,565 amino acids with a predicted molecular mass of approximately 177.8 kDa. The SeCHS-A mRNA was expressed in all developmental stages and specifically in the epidermis and tracheae tissue by quantitative real-time-PCR analysis. Expression of SeCHS-A gene was suppressed by feeding double-stranded RNA (dsCHS-A, 400 ng/larva) in the third instar larvae of S. exigua. Suppression of the SeCHS-A gene expression significantly increased 35% of mortality on pupation of S. exigua. Also, the third instar larvae fed with dsCHS-A significantly increased susceptibility to entomopathogenic fungi, Beauveria bassiana ANU1 at 3 days after treatment. These results suggest that the SeCHS-A gene plays an important role in development of S. exigua and RNA interference may apply to effective pest control with B. bassiana.

  13. Genome-Wide Identification, Characterization and Expression Analysis of the Chalcone Synthase Family in Maize.

    Science.gov (United States)

    Han, Yahui; Ding, Ting; Su, Bo; Jiang, Haiyang

    2016-01-27

    Members of the chalcone synthase (CHS) family participate in the synthesis of a series of secondary metabolites in plants, fungi and bacteria. The metabolites play important roles in protecting land plants against various environmental stresses during the evolutionary process. Our research was conducted on comprehensive investigation of CHS genes in maize (Zea mays L.), including their phylogenetic relationships, gene structures, chromosomal locations and expression analysis. Fourteen CHS genes (ZmCHS01-14) were identified in the genome of maize, representing one of the largest numbers of CHS family members identified in one organism to date. The gene family was classified into four major classes (classes I-IV) based on their phylogenetic relationships. Most of them contained two exons and one intron. The 14 genes were unevenly located on six chromosomes. Two segmental duplication events were identified, which might contribute to the expansion of the maize CHS gene family to some extent. In addition, quantitative real-time PCR and microarray data analyses suggested that ZmCHS genes exhibited various expression patterns, indicating functional diversification of the ZmCHS genes. Our results will contribute to future studies of the complexity of the CHS gene family in maize and provide valuable information for the systematic analysis of the functions of the CHS gene family.

  14. Factors influencing the turnover intention of Chinese community health service workers based on the investigation results of five provinces.

    Science.gov (United States)

    Sun, Yu; Luo, Zhenni; Fang, Pengqian

    2013-12-01

    After the implementation of the new health reform, the Chinese government paid increasing attention to developing its community health service (CHS). The focus is mainly on cultivating community general medical practitioners but paying less attention to the working status and occupational demands of in-service CHS workers. CHS requires a stable team that can provide good service to community residents. At present, the demission rate of CHS workers is high. Studying how to increase the stability of CHS workers is noteworthy. The goal of this study is to ascertain the key factors that influence the CHS worker turnover intention to increase their work satisfaction and stability. A total of 100 CHS organizations were sampled randomly in 10 cities from 5 Chinese provinces for this study. All CHS workers from these organizations took a questionnaire survey. In total, 3,212 valid answer sheets were collected. Pearson Chi square test and Binary logistic regression were used to analyze the related influencing factors that result in CHS worker turnover intention. A total of 38.7% of those who accomplished the questionnaire intended to quit. The influencing factors that result in CHS worker turnover intention are (1) socio-demographic factors such as age, post of duty, professional title, and working seniority, and (2) other work-related factors such as pay packets, learning and training opportunities, promotion and personal development space, and working stress. CHS workers were less satisfied with the balance between payment and work quantity, promotion opportunity, and working conditions. Based on the results, the government should pay more attention to the various demands of CHS workers in service, especially by increasing their income, providing more learning and training opportunities, and increasing the degree of their work satisfaction to avoid turnover intention and ensure the stability of the CHS workforce.

  15. Characterization of direct methanol fuel cell (DMFC) applications with H{sub 2}SO{sub 4} modified chitosan membrane

    Energy Technology Data Exchange (ETDEWEB)

    Osifo, Peter O.; Masala, Aluwani [Department of Chemical Engineering, Vaal University of Technology, Andries Potgieter Bolevald, P/Bag X021, Vanderbijlpark 1900, Gauteng (South Africa)

    2010-08-01

    Chitosan (Chs) flakes were prepared from chitin materials that were extracted from the exoskeleton of Cape rock lobsters in South Africa. The Chs flakes were prepared into membranes and the Chs membranes were modified by cross-linking with H{sub 2}SO{sub 4}. The cross-linked Chs membranes were characterized for the application in direct methanol fuel cells. The Chs membrane characteristics such as water uptake, thermal stability, proton resistance and methanol permeability were compared to that of high performance conventional Nafion 117 membranes. Under the temperature range studied 20-60 C, the membrane water uptake for Chs was found to be higher than that of Nafion. Thermal analysis revealed that Chs membranes could withstand temperature as high as 230 C whereas Nafion 117 membranes were stable to 320 C under nitrogen. Nafion 117 membranes were found to exhibit high proton resistance of 284 s cm{sup -1} than Chs membranes of 204 s cm{sup -1}. The proton fluxes across the membranes were 2.73 mol cm{sup -2} s{sup -1} for Chs- and 1.12 mol cm{sup -2} s{sup -1} Nafion membranes. Methanol (MeOH) permeability through Chs membrane was less, 1.4 x 10{sup -6} cm{sup 2} s{sup -1} for Chs membranes and 3.9 x 10{sup -6} cm{sup 2} s{sup -1} for Nafion 117 membranes at 20 C. Chs and Nafion membranes were fabricated into membrane electrode assemblies (MAE) and their performances measure in a free-breathing commercial single cell DMFC. The Nafion membranes showed a better performance as the power density determined for Nafion membranes of 0.0075 W cm{sup -2} was 2.7 times higher than in the case of Chs MEA. (author)

  16. Transcriptional regulation of the genes encoding chitin and β-1,3-glucan synthases from Ustilago maydis.

    Science.gov (United States)

    Robledo-Briones, Mariana; Ruiz-Herrera, José

    2012-07-01

    Transcriptional regulation of genes encoding chitin synthases (CHS) and β-1,3-glucan synthase (GLS) from Ustilago maydis was studied. Transcript levels were measured during the growth curve of yeast and mycelial forms, in response to ionic and osmotic stress, and during infection of maize plants. Expression of the single GLS gene was constitutive. In contrast, CHS genes expression showed differences depending on environmental conditions. Transcript levels were slightly higher in the mycelial forms, the highest levels occurring at the log phase. Ionic and osmotic stress induced alterations in the expression of CHS genes, but not following a defined pattern, some genes were induced and others repressed by the tested compounds. Changes in transcripts were more apparent during the pathogenic process. At early infection stages, only CHS6 gene showed significant transcript levels, whereas at the period of tumor formation CHS7 and CHS8 genes were also were induced.

  17. AcEST: DK957574 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 61 TGEGLEWGVLFGFGPGLTIETVVLRSV 387 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 SV...raria lobata GN=CHS... 268 2e-71 sp|P30074|CHS2_MEDSA Chalcone synthase 2 OS=Medica...269 1e-71 sp|P51078|CHS5_MEDSA Chalcone synthase 4-2 OS=Medicago sativa GN... 269 1e-71 sp|P23569|CHSY_PUELO Chalcone synthase OS=Pue

  18. Magnetic field of a combined plasma trap

    Science.gov (United States)

    Kotenko, V. G.; Moiseenko, V. E.; Ågren, O.

    2012-06-01

    This paper presents numerical simulations performed on the structure of a magnetic field created by the magnetic system of a combined plasma trap. The magnetic system includes the stellarator-type magnetic system and one of the mirror-type. For the stellarator type magnetic system the numeric model contains a magnetic system of an l=2 torsatron with the coils of an additional toroidal magnetic field. The mirror-type magnetic system element is considered as being single current-carrying turn enveloping the region of existence of closed magnetic surfaces of the torsatron. The calculations indicate the existence of a vast area of the values of the additional magnetic field magnitude and magnetic field of the single turn where, in principle, the implementation of the closed magnetic surface configuration is quite feasible.

  19. Modular stellarator reactor: a fusion power plant

    Energy Technology Data Exchange (ETDEWEB)

    Miller, R.L.; Bathke, C.G.; Krakowski, R.A.; Heck, F.M.; Green, L.; Karbowski, J.S.; Murphy, J.H.; Tupper, R.B.; DeLuca, R.A.; Moazed, A.

    1983-07-01

    A comparative analysis of the modular stellarator and the torsatron concepts is made based upon a steady-state ignited, DT-fueled, reactor embodiment of each concept for use as a central electric-power station. Parametric tradeoff calculations lead to the selection of four design points for an approx. 4-GWt plant based upon Alcator transport scaling in l = 2 systems of moderate aspect ratio. The four design points represent high-aspect ratio. The four design points represent high-(0.08) and low-(0.04) beta versions of the modular stellarator and torsatron concepts. The physics basis of each design point is described together with supporting engineering and economic analyses. The primary intent of this study is the elucidation of key physics and engineering tradeoffs, constraints, and uncertainties with respect to the ultimate power reactor embodiment.

  20. BOOK REVIEW: Stellarator and Heliotron Devices

    Science.gov (United States)

    Johnson, John L.

    1999-02-01

    Pfirsch-Schlüter current driven magnetic islands and the interpretation of sawtooth instabilities in Heliotron E. The treatment of particle orbits in Chapter 6 includes a derivation of drift equations, a discussion of the characteristics of trapped particle confinement in a heliotron and one of the Monte Carlo method for studying transport phenomena. A good treatment of neoclassical transport in a stellarator, with emphasis on the relation between parallel viscosity driven fluxes and bootstrap current, is given in Chapter 7. This is the best treatment I have found, outside of the original references, but it is still demanding. In addition, a radial electric field is introduced into the energy transport equations. The treatment of heating and confinement of heliotron plasmas in Chapter 8 is a good combination of providing results from experiments on the Heliotron E and DR heliotrons and the ATF and CHS stellarators and showing how theoretical interpretation is formulated. The discussions of ray tracing and energy absorption for both ECRH and ICRF heating techniques, as well as a treatment of neutral beam injection, are very clear. Measurements of bootstrap current and plasma rotation, as well as the density limits associated with pellet injection, are discussed. The chapter ends with a discussion of what may be the author's favourite topic, pressure gradient driven turbulence, in which he describes mixing length and scale invariance techniques. Finally, a discussion of the characteristics of a steady state fusion reactor, including a treatment of the containment, slowing down and energy transfer of the alpha particles, one of the toroidal Alfvén modes driven by these particles and some physics of divertors are given in Chapter 9. A reviewer is usually expected to find some faults. I had no problem in finding one as soon as I received the book: indeed, I did not like its title. I have always maintained that Lyman Spitzer defined a stellarator as any toroidal device in

  1. Effect of plagiochin E, an antifungal macrocyclic bis(bibenzyl), on cell wall chitin synthesis in Candida albicans

    Institute of Scientific and Technical Information of China (English)

    Xiu-zhen WU; Ai-xia CHENG; Ling-mei SUN; Hong-xiang LOU

    2008-01-01

    Aim: To investigate the effect of plagiochin E (PLE), an antifungal macrocyclic bis(bibenzyl) isolated from liverwort Marchantia polymorpha L, on cell wall chitin synthesis in Candida albicans. Methods: The effect of PLE on chitin synthesis in Candida albicans was investigated at the cellular and molecular lev-els. First, the ultrastructural changes were observed under transmission electron microscopy (TEM). Second, the effects of PLE on chitin synthetase (Chs) activi-ties in vitro were assayed using 6-O-dansyl-N-acetylglucosamine as a fluorescent substrate, and its effect on chitin synthesis in situ was assayed by spheroplast regeneration. Finally, real-time RT-PCR was performed to assay its effect on the expression of Chs genes (CHS). Results: Observation under TEM showed that the structure of the cell wall in Candida albicans was seriously damaged, which suggested that the antifungal activity of PLE was associated with its effect on the cell wail. Enzymatic assays and spheroplast regeneration showed that PLE inhibited chitin synthesis in vitro and in situ. The results of the PCR showed that PLE significantly downregulated the expression of CHS1, and upregulated the expression of CHS2 and CHS3. Because different Chs is regulated at different stages of transcription and post-translation, the downregulation of CHS1 would decrease the level of Chs 1 and inhibit its activity, and the inhibitory effects of PLE on Chs2 and Chs3 would be at the post-translational level or by the inhibi-tion on the enzyme-active center. Conclusion: These results indicate that the antifungal activity of PLE would be attributed to its inhibitory effect on cell wall chitin synthesis in Candida albicans.

  2. Phonon dispersion relation of liquid metals

    Indian Academy of Sciences (India)

    P B Thakor; P N Gajjar; A R Jani

    2009-06-01

    The phonon dispersion curves of some liquid metals, viz. Na ( = 1), Mg ( = 2), Al ( = 3) and Pb ( = 4), have been computed using our model potential. The charged hard sphere (CHS) reference system is applied to describe the structural information. Our model potential along with CHS reference system is capable of explaining the phonon dispersion relation for monovalent, divalent, trivalent and tetravalent liquid metals.

  3. Art and experience

    NARCIS (Netherlands)

    Gerwen, R.C.H.M. van

    2001-01-01

    This three-part dissertation is on the double role of experience in art: as a subject matter, and as the vehicle for our evaluations. It argues (Part three, Chs. 7, 8) for the inclusion within contemporary analytical ‘cognitivism’ (Part one, Chs. 1-3) of certain arguments from the founding fathers o

  4. Cloning and expression analysis of chalcone synthase gene from Coleus forskohlii.

    Science.gov (United States)

    Awasthi, Praveen; Mahajan, Vidushi; Jamwal, Vijay Lakshmi; Kapoor, Nitika; Rasool, Shafaq; Bedi, Yashbir S; Gandhi, Sumit G

    2016-09-01

    Flavonoids are an important class of secondary metabolites that play various roles in plants such as mediating defense, floral pigmentation and plant-microbe interaction. Flavonoids are also known to possess antioxidant and antimicrobial activities. Coleus forskohlii (Willd.) Briq. (Lamiaceae) is an important medicinal herb with a diverse metabolic profile, including production of a flavonoid, genkwanin. However, components of the flavonoid pathway have not yet been studied in this plant. Chalcone synthase (CHS) catalyses the first committed step of flavonoid biosynthetic pathway. Full-length cDNA, showing homology with plant CHS gene was isolated from leaves of C. forskohlii and named CfCHS (GenBank accession no. KF643243). Theoretical translation of CfCHS nucleotide sequence shows that it encodes a protein of 391 amino acids with a molecular weight of 42.75 kDa and pI 6.57. Expression analysis of CfCHS in different tissues and elicitor treatments showed that methyl jasmonate (MeJA) strongly induced its expression. Total flavonoids content and antioxidant activity of C. forskohlii also got enhanced in response to MeJA, which correlated with increased CfCHS expression. Induction of CfCHS by MeJA suggest its involvement in production of flavonoids, providing protection from microbes during herbivory or mechanical wounding. Further, our in silico predictions and experimental data suggested that CfCHS may be posttranscriptionally regulated by miR34.

  5. Target enzyme mutations are the molecular basis for resistance towards pharmacological inhibition of nicotinamide phosphoribosyltransferase

    DEFF Research Database (Denmark)

    Olesen, Uffe H; Petersen, Jakob G; Garten, Antje;

    2010-01-01

    BACKGROUND: Inhibitors of nicotinamide phosphoribosyltransferase (NAMPT) are promising cancer drugs currently in clinical trials in oncology, including APO866, CHS-828 and the CHS-828 prodrug EB1627/GMX1777, but cancer cell resistance to these drugs has not been studied in detail. METHODS: Here, ...

  6. Flavonols and fertilization in Petunia hybrida: localization and mode of action during pollen tube growth

    NARCIS (Netherlands)

    Ylstra, B.; Busscher, J.; Franken, J.; Hollman, P.C.H.; Mol, J.N.M.; Tunen, van A.J.

    1995-01-01

    Flavonols form an important class of flavonoids which serve an essential function during plant reproduction. Flavonoid biosynthesis is initiated by the enzyme chalcone synthase (CHS). A high abundance of flavonols and chs mRNA was demonstrated in male and female reproductive organs of Petunia hybrid

  7. Metabolic changes in Arabidopsis thaliana plants overexpressing chalcone synthase

    NARCIS (Netherlands)

    Dao, Thi Thanh Hien

    2010-01-01

    The study has shown that it is possible to introduce the heterologous CHS gene in Arabidopsis thaliana and common multicopies of transgenes containing plants were obtained. Analysis of the change in metabolome of CHS transgenic plants, high expression transgenic lines can be identified by markers su

  8. Host-induced gene silencing of an essential chitin synthase gene confers durable resistance to Fusarium head blight and seedling blight in wheat.

    Science.gov (United States)

    Cheng, Wei; Song, Xiu-Shi; Li, He-Ping; Cao, Le-Hui; Sun, Ke; Qiu, Xiao-Li; Xu, Yu-Bin; Yang, Peng; Huang, Tao; Zhang, Jing-Bo; Qu, Bo; Liao, Yu-Cai

    2015-12-01

    Fusarium head blight (FHB) and Fusarium seedling blight (FSB) of wheat, caused by Fusarium pathogens, are devastating diseases worldwide. We report the expression of RNA interference (RNAi) sequences derived from an essential Fusarium graminearum (Fg) virulence gene, chitin synthase (Chs) 3b, as a method to enhance resistance of wheat plants to fungal pathogens. Deletion of Chs3b was lethal to Fg; disruption of the other Chs gene family members generated knockout mutants with diverse impacts on Fg. Comparative expression analyses revealed that among the Chs gene family members, Chs3b had the highest expression levels during Fg colonization of wheat. Three hairpin RNAi constructs corresponding to the different regions of Chs3b were found to silence Chs3b in transgenic Fg strains. Co-expression of these three RNAi constructs in two independent elite wheat cultivar transgenic lines conferred high levels of stable, consistent resistance (combined type I and II resistance) to both FHB and FSB throughout the T3 to T5 generations. Confocal microscopy revealed profoundly restricted mycelia in Fg-infected transgenic wheat plants. Presence of the three specific short interfering RNAs in transgenic wheat plants was confirmed by Northern blotting, and these RNAs efficiently down-regulated Chs3b in the colonizing Fusarium pathogens on wheat seedlings and spikes. Our results demonstrate that host-induced gene silencing of an essential fungal chitin synthase gene is an effective strategy for enhancing resistance in crop plants under field test conditions.

  9. Feature evaluation of complex hysteresis smoothing and its practical applications to noisy SEM images.

    Science.gov (United States)

    Suzuki, Kazuhiko; Oho, Eisaku

    2013-01-01

    Quality of a scanning electron microscopy (SEM) image is strongly influenced by noise. This is a fundamental drawback of the SEM instrument. Complex hysteresis smoothing (CHS) has been previously developed for noise removal of SEM images. This noise removal is performed by monitoring and processing properly the amplitude of the SEM signal. As it stands now, CHS may not be so utilized, though it has several advantages for SEM. For example, the resolution of image processed by CHS is basically equal to that of the original image. In order to find wide application of the CHS method in microscopy, the feature of CHS, which has not been so clarified until now is evaluated correctly. As the application of the result obtained by the feature evaluation, cursor width (CW), which is the sole processing parameter of CHS, is determined more properly using standard deviation of noise Nσ. In addition, disadvantage that CHS cannot remove the noise with excessively large amplitude is improved by a certain postprocessing. CHS is successfully applicable to SEM images with various noise amplitudes.

  10. Association between time of pay-for-performance for patients and community health services use by chronic patients.

    Directory of Open Access Journals (Sweden)

    Xi Sun

    Full Text Available BACKGROUND: Pay-for-performance for patients is a cost-effective means of improving health behaviours. This study examined the association between the pay time for performance for patients and CHS use by chronic patients. METHODS: A cross-sectional study was undertaken to estimate distribution characteristics of CHS use in 2011 and collect data of socio-demographic characteristics (sex, age, education level, occupation, disposable personal income in 2011, distance between home and community health agency, chronic disease number, and time of pay-for-performance for patients. Participants were 889 rural adults with hypertension or type II diabetes aged 35 and above. Standardized CHS use means chronic patients use CHS at least once per quarter. RESULTS: Patients who received incentives prior to services had 2.724 times greater odds of using standardized CHS than those who received incentives after services (95%CI, 1.986-3.736, P<0.001. For all subgroups (socio-demographic characteristics and chronic disease number, patients who received incentives prior to services were more likely to use standardized CHS than those receiving incentives after services. CONCLUSIONS: Pay time for performance for patients was associated with CHS use by chronic patients. Patients receiving incentive prior to services were more likely to use standardized CHS. And pay time should not be ignored when the policy on pay-for-performance for patients is designed.

  11. Cloning and expression analysis of chalcone synthase gene from Coleus forskohlii

    Indian Academy of Sciences (India)

    PRAVEEN AWASTHI; VIDUSHI MAHAJAN; VIJAY LAKSHMI JAMWAL; NITIKA KAPOOR; SHAFAQ RASOOL; YASHBIR S. BEDI; SUMIT G. GANDHI

    2016-09-01

    Flavonoids are an important class of secondary metabolites that play various roles in plants such as mediating defense, floral pigmentation and plant–microbe interaction. Flavonoids are also known to possess antioxidant and antimicrobial activities. Coleus forskohlii (Willd.) Briq. (Lamiaceae) is an important medicinal herb with a diverse metabolic profile, including production of a flavonoid, genkwanin. However, components of the flavonoid pathway have not yet been studied in this plant. Chalcone synthase (CHS) catalyses the first committed step of flavonoid biosynthetic pathway. Full-length cDNA, showing homology with plantCHS gene was isolated from leaves of C. forskohlii and named CfCHS (GenBank accession no. KF643243). Theoretical translation of CfCHS nucleotide sequence shows that it encodes a protein of 391 amino acids with a molecular weight of 42.75 kDa and pI 6.57. Expression analysis of CfCHS in different tissues and elicitor treatments showed that methyl jasmonate (MeJA) strongly induced its expression. Total flavonoids content and antioxidant activity of C.forskohlii also got enhanced in response to MeJA, which correlated with increased CfCHS expression. Induction ofCfCHS by MeJA suggest its involvement in production of flavonoids, providing protection from microbes during herbivory or mechanical wounding. Further, ourin silico predictions and experimental data suggested that CfCHS may be posttranscriptionally regulated by miR34.

  12. AcEST: DK961108 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS... 279 8e-75 sp|P30074|CHS2_MEDSA Chalcone synt...MSSACVLFILDE 348 Query: 542 LRKKSVE 562 +RKKS + Sbjct: 349 MRKKSAQ 355 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria

  13. AcEST: DK953186 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ense GN=C... 218 2e-56 sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS... 215 2e-55 sp|P300...T 662 +FCT Sbjct: 134 VFCT 137 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 SV=1 L

  14. Fatigue strength of truss girders made of very high strength steel

    NARCIS (Netherlands)

    Pijpers, R.J.M.; Kolstein, M.H.

    2010-01-01

    An effective application of Very High Strength Steel (VHSS) in civil engineering structures is expected in stiff, truss like structures, typically made of Circular Hollow Sections (CHS). Use of castings in combination with CHS could be promising for the design of highly fatigue resistant joints. Cas

  15. Referral patterns between the child health care, general practitioners and secondary health care: a prospective descriptive study in the Netherlands

    NARCIS (Netherlands)

    Einhorn, R.; Eekhof, J.A.H. van; Engelberts, A.C.; Groeneveld, Y.; Verkerk, P.H.; Wit, J.M.

    2007-01-01

    Background: In the Netherlands, preventive child health service (CHS) screening plays an important role in the early detection of congenital, developmental, physical, and mental disorders. Objective: To obtain insight into the referral patterns of children from CHS to general practitioners and from

  16. Living Democracy: How Constitution High School Molds Better Citizens

    Science.gov (United States)

    Brasof, Marc

    2009-01-01

    Philadelphia's Constitution High School (CHS) is committed both to the theory of education for democracy, and to its practice, as reflected by a school constitution, student elections, town hall meetings, and active student participation in school government. As its name indicates, CHS is a theme-based high school that focuses on history,…

  17. Chediak Higashi Syndrome masquerading as acute leukemia / storage disorder - A rare case report

    Directory of Open Access Journals (Sweden)

    Mirza Asif Baig

    2015-07-01

    CHS is characterised by partial oculocutaneous albinism, frequent fatal bacterial infections, bleeding diathesis and peripheral + Cranial nerve palsies. This disorder further culminates into accelerated phase (Lymphoproliferative Syndrome progressing into pancytopenia. CHS is due to single gene mutation in LYST (CHS gene localized to 1q chromosome. The diagnostic hallmark of CHS is presence of giant purple to blue violet inclusions in leucocytes. In this study granules are more prominent in Bone marrow than in PBS correlating well with previous studies. Approximately 85% of the cases, of CHS culminates into Accelerated phase showing Lymphohistiocytic infiltration progressing to pancytopenia and death due to infection. The very rare nature of this disease and its grave prognosis merits its reporting. [Int J Res Med Sci 2015; 3(7.000: 1785-1787

  18. Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity

    DEFF Research Database (Denmark)

    Larsen, Jeppe Madura; Geisler, Carsten; Nielsen, Martin Weiss;

    2007-01-01

    BACKGROUND: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody-induced depletion of specific subtypes of ...... to evaluate the effect of different contact allergens on various cell populations of the immune system. CONCLUSIONS: Our study support recent findings that several cell types seem to be involved in CHS.......BACKGROUND: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody-induced depletion of specific subtypes...

  19. Delayed phagocytosis and bacterial killing in Chediak-Higashi syndrome neutrophils detected by a fluorochrome assay: ultrastructural aspects

    Directory of Open Access Journals (Sweden)

    Raquel Bellinati-Pires

    1992-12-01

    Full Text Available The few studies already published about phagocyte functions in Chediak-Higashi syndrome (CHS has stated that neutrophils present slow rate of bacterial killing but normally ingest microorganisms. In the present study, both phagocytosis and killing of Staphylococcus aureus were verified to be in neutrophils from two patients with CHS when these functions were simultaneously evaluated by a fluorochrome phagocytosis assay. Electron microscopic examination showed morphologic differences among neutophils from CHS patients and normal neutrophils regarding the cytoplasmic structures and the aspects of the phagolysosomes. It was noteworthy the presence of giant phagolysosomes enclosing bacteria in active proliferation commonly observed in CHS neutrophils after 45 min of phagocytosis, wich corresponded with the impaired bactericidal activity of these leukocytes. The present results suggest that phagocytosis may also be defective in CHS, and point out to the sensitivity of the fluorochrome phagocytosis assay and its application in clinical laboratories.

  20. Cannabinoid hyperemesis syndrome. A report of six new cases and a summary of previous reports.

    Science.gov (United States)

    Contreras Narváez, Carla; Mola Gilbert, Montserrat; Batlle de Santiago, Enric; Bigas Farreres, Jordi; Giné Serven, Eloy; Cañete Crespillo, Josep

    2016-03-02

    Cannabinoid hyperemesis syndrome (CHS) is a medical condition which was identified for the first time in 2004 and affects chronic users of cannabis. It is characterized by cyclic episodes of uncontrollable vomiting as well as compulsive bathing in hot water. The episodes have a duration of two to four days. The vomiting is recognizable by a lack of response to regular antiemetic treatment, and subsides only with cannabis abstinence, reappearing in periods of consumption of this substance. The etiology of this syndrome is unknown. Up until June 2014, 83 cases of CHS were published worldwide, four of them in Spain.The first patient of CHS at Mataró Hospital was diagnosed in 2012. Since then, five new cases have been identified. The average duration between the onset of acute CHS episodes and diagnosis is 6.1 years, similar to that observed in previously published cases, an average of 3.1 years. This "delay" of CHS diagnosis demonstrates a lack of awareness with respect to this medical condition in the healthcare profession.With the objective of providing information concerning CHS and facilitating its timely diagnosis, a series of six new cases of CHS diagnosed in Mataró Hospital is presented along with a summary of cases published between 2004 and June 2014.

  1. Evolution of the chitin synthase gene family correlates with fungal morphogenesis and adaption to ecological niches

    Science.gov (United States)

    Liu, Ran; Xu, Chuan; Zhang, Qiangqiang; Wang, Shiyi; Fang, Weiguo

    2017-01-01

    The fungal kingdom potentially has the most complex chitin synthase (CHS) gene family, but evolution of the fungal CHS gene family and its diversification to fulfill multiple functions remain to be elucidated. Here, we identified the full complement of CHSs from 231 fungal species. Using the largest dataset to date, we characterized the evolution of the fungal CHS gene family using phylogenetic and domain structure analysis. Gene duplication, domain recombination and accretion are major mechanisms underlying the diversification of the fungal CHS gene family, producing at least 7 CHS classes. Contraction of the CHS gene family is morphology-specific, with significant loss in unicellular fungi, whereas family expansion is lineage-specific with obvious expansion in early-diverging fungi. ClassV and ClassVII CHSs with the same domain structure were produced by the recruitment of domains PF00063 and PF08766 and subsequent duplications. Comparative analysis of their functions in multiple fungal species shows that the emergence of ClassV and ClassVII CHSs is important for the morphogenesis of filamentous fungi, development of pathogenicity in pathogenic fungi, and heat stress tolerance in Pezizomycotina fungi. This work reveals the evolution of the fungal CHS gene family, and its correlation with fungal morphogenesis and adaptation to ecological niches. PMID:28300148

  2. Two Novel Mutations Identified in an African-American Child with Chediak-Higashi Syndrome

    Directory of Open Access Journals (Sweden)

    Kerry Morrone

    2010-01-01

    Full Text Available Background. Chediak-Higashi syndrome (CHS is a rare, autosomal recessive disorder characterized by oculocutaneous albinism, immunodeficiency, coagulopathy and late-onset, progressive neurological dysfunction. It also has an “accelerated phase” characterized by hemophagocytic lymphohistiocytosis (HLH. The disease is caused by mutations in the CHS1/LYST gene located on chromosome 1, which affects lysosome morphology and function. We report the case of an African-American child with CHS in Case. This 16-month old African-American girl presented with fever and lethargy. The proband had pale skin compared to her parents, with light brown eyes, silvery hair and massive hepatosplenomegaly. Her laboratory evaluation was remarkable for pancytopenia, high serum ferritin and an elevated LDH. Bone marrow aspirate revealed large inclusions in granulocytes and erythrophagocytosis consistent with HLH. Genetic evaluation revealed two novel nonsense mutations in the CHS1 gene: c.3622C>T (p.Q1208X and c.11002G>T (p.E3668X. Conclusions. Our patient is one of the few cases of CHS reported in the African American population. We identified 2 nonsense mutations in the CHS1 gene, the first mutation analysis published of an African-American child with Chediak-Higashi Syndrome. These two mutations predict a severe phenotype and thus identification of these mutations has an important clinical significance in CHS.

  3. Exposure to Diflubenzuron Results in an Up-Regulation of a Chitin Synthase 1 Gene in Citrus Red Mite, Panonychus citri (Acari: Tetranychidae

    Directory of Open Access Journals (Sweden)

    Wen-Kai Xia

    2014-02-01

    Full Text Available Chitin synthase synthesizes chitin, which is critical for the arthropod exoskeleton. In this study, we cloned the cDNA sequences of a chitin synthase 1 gene, PcCHS1, in the citrus red mite, Panonychus citri (McGregor, which is one of the most economically important pests of citrus worldwide. The full-length cDNA of PcCHS1 contains an open reading frame of 4605 bp of nucleotides, which encodes a protein of 1535 amino acid residues with a predicted molecular mass of 175.0 kDa. A phylogenetic analysis showed that PcCHS1 was most closely related to CHS1 from Tetranychus urticae. During P. citri development, PcCHS1 was constantly expressed in all stages but highly expressed in the egg stage (114.8-fold higher than in the adult. When larvae were exposed to diflubenzuron (DFB for 6 h, the mite had a significantly high mortality rate, and the mRNA expression levels of PcCHS1 were significantly enhanced. These results indicate a promising use of DFB to control P. citri, by possibly acting as an inhibitor in chitin synthesis as indicated by the up-regulation of PcCHS1 after exposure to DFB.

  4. Assessment of perfluorinated compounds (PFCs) in plasma of bottlenose dolphins from two southeast US estuarine areas: relationship with age, sex and geographic locations.

    Science.gov (United States)

    Fair, Patricia A; Houde, Magali; Hulsey, Thomas C; Bossart, Gregory D; Adams, Jeff; Balthis, Len; Muir, Derek C G

    2012-01-01

    Plasma PFCs were measured in 157 bottlenose dolphins (Tursiops truncatus) sampled from two US southeast Atlantic sites (Charleston (CHS), SC and Indian River Lagoon (IRL), FL) during 2003-2005. ∑PFCs, perfluoroalkyl carboxylates (∑PFCAs), perfluoroalkyl sulfonates (∑PFSAs) and individual compounds were significantly higher in CHS dolphins for all age/sex categories compared to IRL dolphins. Highest ∑PFCs concentrations occurred in CHS juvenile dolphins (2340 ng/g w.w.); significantly higher than found in adults (1570 ng/g w.w. males; 1330 ng/g w.w. females). ∑PFCAs were much greater in CHS dolphins (≈ 21%) compared to IRL dolphins (≈ 7%); ∑PFSAs were 79% in CHS dolphins versus 93% in IRL dolphins. PFOS, the dominant compound, averaged 72% and 84%, respectively, in CHS and IRL dolphins. Decreasing PFC levels occurred with age on the bioaccumulation of PFCs in both sites. These observations suggest PFC accumulation in these two dolphin populations are influenced by site-specific exposures with significantly higher levels in CHS dolphins.

  5. Intraoperative dexmedetomidine and postoperative cerebral hyperperfusion syndrome in patients who underwent superficial temporal artery-middle cerebral artery anastomosis for moyamoya disease

    Science.gov (United States)

    Seo, Hyungseok; Ryu, Ho-Geol; Son, Je Do; Kim, Jeong-Soo; Ha, Eun Jin; Kim, Jeong-Eun; Park, Hee-Pyoung

    2016-01-01

    Abstract Dexmedetomidine, a selective α2-agonist, reduces cerebral blood flow and has neuroprotective effects against cerebral ischemia/reperfusion injury in experimental animals. We examined whether intraoperative dexmedetomidine would reduce the incidence of postoperative cerebral hyperperfusion syndrome (CHS) after superficial temporal artery-middle cerebral artery (STA-MCA) anastomosis in patients with moyamoya disease. The electronic medical records of 117 moyamoya patients who underwent STA-MCA anastomosis were reviewed retrospectively. The patients were divided into 2 groups: 48 patients received intraoperative dexmedetomidine (Group D), while 69 patients did not (Group ND). The incidence (primary outcome), onset, and duration of postoperative CHS were noted. The incidence of postoperative CHS was 45.8% and 40.6% in groups D and ND, respectively (P = 0.708). The duration of postoperative CHS was shorter in group D than in group ND (median [Q1–Q3], 5 [3–7] vs 8 [5–10] days, P = 0.021). There was no significant difference in the onset of CHS between group D and group ND (0 [0–2] vs 1 [0–3] days, P = 0.226). In conclusion, intraoperative dexmedetomidine did not reduce the incidence of postoperative CHS, although it reduced the duration of CHS, in patients who had undergone direct revascularization surgery for moyamoya disease. PMID:28033272

  6. Expression in E. coli and characterization of the catalytic domain of Botrytis cinerea chitin synthase

    Directory of Open Access Journals (Sweden)

    Piffeteau Annie

    2010-11-01

    Full Text Available Abstract Background Chitin synthase 3a (CHS3a from Botrytis cinerea (Bc catalyses the multiple transfer of N-acetylglucosamine (GlcNAc residues to the growing chitin chain. Chitin, a β-1,4 linked GlcNAc homopolymer, is an essential cell wall component of filamentous fungi. Chitin synthase, processive membranous protein, has been recognized as a promising target for new antifungicides. Enzymatic characterizations of chitin synthases have been limited, mainly because purity and amounts of integral enzyme obtained after purification procedures have not been sufficient. Findings We undertook the preparation of two BcCHS3a fragment proteins, containing only the central domain and devoid of the N-terminal and transmembrane C-terminal regions. The central domain of CHS3a, named SGC (Spsa GntI Core, is conserved in all UDP-glycosyltransferases and it is believed to contain the active site of the enzyme. CHS3a-SGC protein was totally expressed as inclusion bodies in Escherichia coli. We performed recombinant CHS3a-SGC purification in denaturing conditions, followed by a refolding step. Although circular dichroism spectra clearly exhibited secondary structures of renatured CHS3a-SGC, no chitin synthase activity was detected. Nevertheless CHS3a-SGC proteins show specific binding for the substrate UDP-GlcNAc with a dissociation constant similar to the Michaelis constant and a major contribution of the uracil moiety for recognition was confirmed. Conclusions Milligram-scale quantities of CHS3a-SGC protein with native-like properties such as specific substrate UDP-GlcNAc binding could be easily obtained. These results are encouraging for subsequent heterologous expression of full-length CHS3a.

  7. The Evolution and Space Weather Effects of Solar Coronal Holes

    Science.gov (United States)

    Krista, Larisza; Gallagher, P.

    2011-05-01

    As solar activity is the foremost important aspect of space weather, the forecasting of flare and CME related transient geomagnetic storms has become a primary initiative. Minor magnetic storms caused by coronal holes (CHs) have also proven to be important due to their long-lasting and recurrent geomagnetic effects. In order to forecast CH related geomagnetic storms, the author developed the Coronal Hole Automated Recognition and Monitoring (CHARM) algorithm to replace the user-dependent CH detection methods commonly used. CHARM uses an intensity thresholding method to identify low intensity regions in EUV or X-ray images. Since CHs are regions of "open” magnetic field and predominant polarity, magnetograms were used to differentiate CHs from other low intensity regions. The Coronal Hole Evolution (CHEVOL) algorithm was developed and used in conjunction with CHARM to study the boundary evolution of CHs. It is widely accepted that the short-term changes in CH boundaries are due to the interchange reconnection between the CH open field lines and small loops. We determined the magnetic reconnection rate and the diffusion coefficient at CH boundaries in order to test the interchange reconnection model. The author also developed the Minor Storm (MIST) package to link CHs to high-speed solar wind (HSSW) periods detected at Earth. Using the algorithm the relationship between CHs, the corresponding HSSW properties, and geomagnetic indices were studied between 2000-2009. The results showed a strong correlation between the velocity and HSSW proton plasma temperature, which indicates that the heating and acceleration of the solar wind plasma in CHs are closely related, and perhaps caused by the same mechanism. The research presented here includes analysis of CHs on small and large spatial/temporal scales, allowing us to further our understanding of CHs as a whole.

  8. Characterization of a Chitin Synthase Encoding Gene and Effect of Diflubenzuron in Soybean Aphid, Aphis Glycines

    Directory of Open Access Journals (Sweden)

    Raman Bansal, M. A. Rouf Mian, Omprakash Mittapalli, Andy P. Michel

    2012-01-01

    Full Text Available Chitin synthases are critical enzymes for synthesis of chitin and thus for subsequent growth and development in insects. We identified the cDNA of chitin synthase gene (CHS in Aphis glycines, the soybean aphid, which is a serious pest of soybean. The full-length cDNA of CHS in A. glycines (AyCHS was 5802 bp long with an open reading frame of 4704 bp that encoded for a 1567 amino acid residues protein. The predicted AyCHS protein had a molecular mass of 180.05 kDa and its amino acid sequence contained all the signature motifs (EDR, QRRRW and TWGTR of chitin synthases. The quantitative real-time PCR (qPCR analysis revealed that AyCHS was expressed in all major tissues (gut, fat body and integument; however, it had the highest expression in integument (~3.5 fold compared to gut. Interestingly, the expression of AyCHS in developing embryos was nearly 7 fold higher compared to adult integument, which probably is a reflection of embryonic molts in hemimetabolus insects. Expression analysis in different developmental stages of A. glycines revealed a consistent AyCHS expression in all stages. Further, through leaf dip bioassay, we tested the effect of diflubenzuron (DFB, Dimilin ®, a chitin-synthesis inhibitor, on A. glycines' survival, fecundity and body weight. When fed with soybean leaves previously dipped in 50 ppm DFB solution, A. glycines nymphs suffered significantly higher mortality compared to control. A. glycines nymphs feeding on diflubenzuron treated leaves showed a slightly enhanced expression (1.67 fold of AyCHS compared to nymphs on untreated leaves. We discussed the potential applications of the current study to develop novel management strategies using chitin-synthesis inhibitors and using RNAi by knocking down AyCHS expression.

  9. Simultaneous post-transcriptional gene silencing of two different chalcone synthase genes resulting in pure white flowers in the octoploid dahlia.

    Science.gov (United States)

    Ohno, Sho; Hosokawa, Munetaka; Kojima, Misa; Kitamura, Yoshikuni; Hoshino, Atsushi; Tatsuzawa, Fumi; Doi, Motoaki; Yazawa, Susumu

    2011-11-01

    Garden dahlias (Dahlia variabilis) are autoallooctoploids with redundant genes producing wide color variations in flowers. There are no pure white dahlia cultivars, despite its long breeding history. However, the white areas of bicolor flower petals appear to be pure white. The objective of this experiment was to elucidate the mechanism by which the pure white color is expressed in the petals of some bicolor cultivars. A pigment analysis showed that no flavonoid derivatives were detected in the white areas of petals in a star-type cultivar 'Yuino' and the two seedling cultivars 'OriW1' and 'OriW2' borne from a red-white bicolor cultivar, 'Orihime', indicating that their white areas are pure white. Semi-quantitative RT-PCR showed that in the pure white areas, transcripts of two chalcone synthases (CHS), DvCHS1 and DvCHS2 which share 69% nucleotide similarity with each other, were barely detected. Premature mRNA of DvCHS1 and DvCHS2 were detected, indicating that these two CHS genes are silenced post-transcriptionally. RNA gel blot analysis revealed that small interfering RNAs (siRNAs) derived from CHSs were produced in these pure white areas. By high-throughput sequence analysis of small RNAs in the pure white areas with no mismatch acceptance, small RNAs were mapped to two alleles of DvCHS1 and two alleles of DvCHS2 expressed in 'Yuino' petals. Therefore, we concluded that simultaneous siRNA-mediated post-transcriptional gene silencing of redundant CHS genes results in the appearance of pure white color in dahlias.

  10. Comparison of Charlson's weighted index of comorbidities with the chronic health score for the prediction of mortality in septic patients

    Institute of Scientific and Technical Information of China (English)

    Cui Yunliang; Wang Tao; Bao Jun; Tian Zhaotao; Lin Zhaofen; Chen Dechang

    2014-01-01

    Background Comorbidity is one of the most important determinants of short-term and long-term outcomes in septic patients.Charlson's weighted index of comorbidities (WIC) and the chronic health score (CHS),which is a component of the acute physiology and chronic health evaluation (APACHE) Ⅱ,are two frequently-used measures of comorbidity.In this study,we assess the performance of WIC and CHS in predicting the hospital mortality of intensive care unit (ICU) patients with sepsis.Methods A total of 338 adult patients with sepsis were admitted to a multisystem ICU between October 2010 and August 2012.Clinical data were collected,including age,gender,underlying diseases,key predisposing causes,severity-of-sepsis,and hospital mortality.The APACHE Ⅱ,CHS,acute physiology score (APS),sequential organ failure assessment (SOFA) and WIC scores were assessed within the first 24 hours of admission.Univariate and multiple Logistic regression analyses were used to compare the performance of WIC and CHS.The area under the receiver operating characteristic curve (AUC) was used to predict hospital mortality over classes of risk.Results Of all the enrolled patients,224 patients survived and 114 patients died.The surviving patients had significantly lower WIC,CHS,APACHE Ⅱ,and SOFA scores than the non-surviving patients (P <0.05).Combining WIC or CHS with other administrative data showed that the hospital mortality was significantly associated with age,severe sepsis,key predisposing causes such as pneumonia,a history of underlying diseases such as hypertension and congestive cardiac failure,and WIC,CHS and APS scores (P <0.05).The AUC for the hospital mortality were 0.564 (95% confidence interval (Cl) 0.496-0.631) of CHS,0.663 (95% Cl 0.599-0.727) of WIC,0.770 (95% Cl 0.718-0.822) of APACHE Ⅱ,0.856 (95% Cl 0.815-0.897) of the CHS combined with other administrative data,and 0.857 (95% Cl 0.817-0.897) of the WIC combined with other administrative data.The diagnostic

  11. Treatment of the X-linked lymphoproliferative, Griscelli and Chédiak-Higashi syndromes by HLH directed therapy

    DEFF Research Database (Denmark)

    Trottestam, Helena; Beutel, Karin; Meeths, Marie;

    2009-01-01

    be used for these syndromes. PROCEDURE: In the HLH-94/HLH-2004 treatment study registries, we evaluated all patients with GS2 (n = 5), XLP (n = 2) or CHS (n = 2) treated between 1994 and 2004. RESULTS: All patients responded to the therapy, and all are alive but one (suffering from CHS), with a mean...... follow-up of 5.6 years. All GS2, one XLP and one CHS patient underwent hematopoietic stem cell transplant. Mean follow-up post transplant was 6.0 years. Six of the seven transplanted children achieved non-active disease status at the time for SCT. Neurological sequelae were reported in all, except...

  12. Multimodal neuromonitoring in pediatric cardiac anesthesia

    Directory of Open Access Journals (Sweden)

    Alexander J. C. Mittnacht

    2014-01-01

    Full Text Available Despite significant improvements in overall outcome, neurological injury remains a feared complication following pediatric congenital heart surgery (CHS. Only if adverse events are detected early enough, can effective actions be initiated preventing potentially serious injury. The multifactorial etiology of neurological injury in CHS patients makes it unlikely that one single monitoring modality will be effective in capturing all possible threats. Improving current and developing new technologies and combining them according to the concept of multimodal monitoring may allow for early detection and possible intervention with the goal to further improve neurological outcome in children undergoing CHS.

  13. Advanced fusion concepts: project summaries

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-12-01

    This report contains descriptions of the activities of all the projects supported by the Advanced Fusion Concepts Branch of the Office of Fusion Energy, US Department of Energy. These descriptions are project summaries of each of the individual projects, and contain the following: title, principle investigators, funding levels, purpose, approach, progress, plans, milestones, graduate students, graduates, other professional staff, and recent publications. Information is given for each of the following programs: (1) reverse-field pinch, (2) compact toroid, (3) alternate fuel/multipoles, (4) stellarator/torsatron, (5) linear magnetic fusion, (6) liners, and (7) Tormac. (MOW)

  14. Pulsed supersonic helium beams for plasma edge diagnosis

    Science.gov (United States)

    Diez-Rojo, T.; Herrero, V. J.; Tanarro, I.; Tabarés, F. L.; Tafalla, D.

    1997-03-01

    An experimental setup for the production of pulsed supersonic He beams to be used for plasma edge diagnosis in fusion devices is described. A compromise between compact design, low cost, and good quality of the probe beams has been met. The main characteristics of the generated beams, such as pulse shape, absolute flux intensity, and velocity distribution, differ in general from those expected for ideal beam performance and have been determined and optimized experimentally. A first test of this He beam source at the TJ-I UP Torsatron in Madrid is also reported.

  15. Pulsed supersonic helium beams for plasma edge diagnosis

    Energy Technology Data Exchange (ETDEWEB)

    Diez-Rojo, T.; Herrero, V.J.; Tanarro, I. [Instituto de Estructura de la Materia (CSIC), Serrano 123, 28006 Madrid (Spain); Tabares, F.L.; Tafalla, D. [Asociacion EURATOM-CIEMAT para Fusion, Avenue Complutense 22, 28040 Madrid (Spain)

    1997-03-01

    An experimental setup for the production of pulsed supersonic He beams to be used for plasma edge diagnosis in fusion devices is described. A compromise between compact design, low cost, and good quality of the probe beams has been met. The main characteristics of the generated beams, such as pulse shape, absolute flux intensity, and velocity distribution, differ in general from those expected for ideal beam performance and have been determined and optimized experimentally. A first test of this He beam source at the TJ-I UP Torsatron in Madrid is also reported. {copyright} {ital 1997 American Institute of Physics.}

  16. Final Report: Cartanega Lagoon Contaminants Study

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Organochlorine analysis did not reveal any 0CH traces in the sediments. Low traces of some 0CHs including DDE were found in both bird liver samples (Appendix 2). DDE...

  17. Influence of coronal holes on CMEs in causing SEP events

    Institute of Scientific and Technical Information of China (English)

    Cheng-Long Shen; Jia Yao; Yu-Ming Wang; Pin-Zhong Ye; Xue-Pu Zhao; Shui Wang

    2010-01-01

    The issue of the influence of coronal holes(CHs)on coronal mass ejections(CMEs)in causing solar energetic particle(SEP)events is revisited.It is a continuation and extension of our previous work,in which no evident effects of CHs on CMEs in generating SEPs were found by statistically investigating 56 CME events.This result is consistent with the conclusion obtained by Kahler in 2004.We extrapolate the coronal magnetic field,define CHs as the regions consisting of only open magnetic field lines and perform a similar analysis on this issue for 76 events in total by extending the study interval to the end of 2008.Three key parameters,CH proximity,CH area and CH relative position,are involved in the analysis.The new result confirms the previous conclusion that CHs did not show any evident effect on CMEs in causing SEP events.

  18. Chronic Toxoplasmosis Modulates the Induction of Contact Hypersensitivity by TNCB in Mouse Model.

    Science.gov (United States)

    Yang, Zhaoshou; Ahn, Hye-Jin; Nam, Ho-Woo

    2015-12-01

    Mouse models of chronic toxoplasmosis and atopic dermatitis (AD) were combined to clarify the effect of opportunistic Toxoplasma gondii infection on the development of AD. AD was induced as a chronic contact hypersensitivity (CHS) with repeated challenge of 2,4,6-trinitro-1-chlorobenzene (TNCB) on the dorsal skin of mice. TNCB induced skin thickness increases in both normal and toxoplasmic mice. The changing patterns were different from the sigmoidal which saturated at 20 days in normal mice to the convex saturated at 12 days in toxoplasmic mice with the crossing at 18 days. Compared to normal mice, toxoplasmic mice presented CHS more severely in earlier times and then moderately in later times. These data suggest that host immune modification by T. gondii infection enhances CHS in early times of atopic stimulation but soothes the reaction of CHS in later times in mouse model.

  19. An Overlooked Victim of Cannabis: Losing Several Years of Well-being and Inches of Jejunum on the Way to Unravel Her Hyperemesis Enigma.

    Science.gov (United States)

    Bonnet, Udo

    2016-01-01

    A case report of a severe cannabis hyperemesis syndrome (CHS) is presented, which had worsened during dronabinol administration and was associated with intestinal dysmotility (pseudo-obstruction). Because dronabinol is an isomer of THC (delta-9-tetrahydrocannabinol), the main psychotropic constituent of cannabis, this case provides first direct clinical evidence on the key role of THC in the obscure pathogenesis of CHS. Another peculiarity of this case was that the patient had an odyssey of hospital stays with extensive workups before the patient herself found via Internet the right diagnosis for her cyclic vomiting and abdominal pain. This is typical for CHS, which is often overlooked because physicians refer to the widely known antiemetic properties of cannabis, for example, in cancer chemotherapy but were not always aware of a possible paradoxical emetic reaction of recreational cannabis use. Being pathognomonic of CHS, the patient became symptom-free while abstaining from her cannabis use, meanwhile being in her 12th month of controlled abstinence.

  20. Cholesterol tuning of BK ethanol response is enantioselective, and is a function of accompanying lipids.

    Directory of Open Access Journals (Sweden)

    Chunbo Yuan

    Full Text Available In the search to uncover ethanol's molecular mechanisms, the calcium and voltage activated, large conductance potassium channel (BK has emerged as an important molecule. We examine how cholesterol content in bilayers of 1,2-dioleoyl-3-phosphatidylethanolamine (DOPE/sphingomyelin (SPM and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine (POPE/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylserine (POPS affect the function and ethanol sensitivity of BK. In addition, we examine how manipulation of cholesterol in biological membranes modulates ethanol's actions on BK. We report that cholesterol levels regulate the change in BK channel open probability elicited by 50 mM ethanol. Low levels of cholesterol (<20%, molar ratio supports ethanol activation, while high levels of cholesterol leads to ethanol inhibition of BK. To determine if cholesterol affects BK and its sensitivity to ethanol through a direct cholesterol-protein interaction or via an indirect action on the lipid bilayer, we used the synthetic enantiomer of cholesterol (ent-CHS. We found that 20% and 40% ent-CHS had little effect on the ethanol sensitivity of BK, when compared with the same concentration of nat-CHS. We accessed the effects of ent-CHS and nat-CHS on the molecular organization of DOPE/SPM monolayers at the air/water interface. The isotherm data showed that ent-CHS condensed DOPE/SPM monolayer equivalently to nat-CHS at a 20% concentration, but slightly less at a 40% concentration. Atomic force microscopy (AFM images of DOPE/SPM membranes in the presence of ent-CHS or nat-CHS prepared with LB technique or vesicle deposition showed no significant difference in topographies, supporting the interpretation that the differences in actions of nat-CHS and ent-CHS on BK channel are not likely from a generalized action on bilayers. We conclude that membrane cholesterol influences ethanol's modulation of BK in a complex manner, including an interaction with the channel protein

  1. Report of the Defense Science Board on Use of Commercial Components in Military Equipment

    Science.gov (United States)

    1989-06-01

    and logistica support that uws short. concise. easy to understand and streamlined. A-33 CHS RFP OVERVIEW THE CHS SOLICITATION IS: * UNIQUE...INCLUDE A PORTABLE COMPUTER AND A TRANSPORTABLE COMPUTER IN TWO DEGRESS OF RUGGEDNESS. AND A HANDHELD TERMINAL I FFP CONTRACT (DAABO7-88-C-JOIS) AWARDED TO...Software Handhold Terminal Unit (HTU) ATCCS Comm Operating System (ACOS) portable Computer Unit (FCU) a Unix SVID Transportable Computer Unit (TCU

  2. Prediction of Cerebral Hyperperfusion Syndrome with Velocity Blood Pressure Index

    Directory of Open Access Journals (Sweden)

    Zhi-Chao Lai

    2015-01-01

    Full Text Available Background: Cerebral hyperperfusion syndrome is an important complication of carotid endarterectomy (CEA. An >100% increase in middle cerebral artery velocity (MCAV after CEA is used to predict the cerebral hyperperfusion syndrome (CHS development, but the accuracy is limited. The increase in blood pressure (BP after surgery is a risk factor of CHS, but no study uses it to predict CHS. This study was to create a more precise parameter for prediction of CHS by combined the increase of MCAV and BP after CEA. Methods: Systolic MCAV measured by transcranial Doppler and systematic BP were recorded preoperatively; 30 min postoperatively. The new parameter velocity BP index (VBI was calculated from the postoperative increase ratios of MCAV and BP. The prediction powers of VBI and the increase ratio of MCAV (velocity ratio [VR] were compared for predicting CHS occurrence. Results: Totally, 6/185 cases suffered CHS. The best-fit cut-off point of 2.0 for VBI was identified, which had 83.3% sensitivity, 98.3% specificity, 62.5% positive predictive value and 99.4% negative predictive value for CHS development. This result is significantly better than VR (33.3%, 97.2%, 28.6% and 97.8%. The area under the curve (AUC of receiver operating characteristic: AUC VBI = 0.981, 95% confidence interval [CI] 0.949-0.995; AUC VR = 0.935, 95% CI 0.890-0.966, P = 0.02. Conclusions: The new parameter VBI can more accurately predict patients at risk of CHS after CEA. This observation needs to be validated by larger studies.

  3. Temporal effect of local hyperthermia on murine contact hypersensitivity

    Institute of Scientific and Technical Information of China (English)

    ZHANG Lan; WANG Yi-ru; HONG Yu-xiao; XU Ya-qin; ZHANG Li; LI Xiao-dong; XIAO Ting

    2013-01-01

    Background The sensitization and elicitation phases are involved in the immunopathogenesis of contact hypersensitivity (CHS).Langerhans cells (LCs) are believed to play pivotal roles in the sensitization stage of CHS.Local hyperthermia on skin induces the migration as well as maturation of epidermal LCs.Although fever-range whole body hyperthermia and local hyperthermia at 43℃ prior to sensitization were reported to suppress CHS,the effects of different temperatures and the timing sequence of local hyperthermia on CHS have not been tackled.Methods Local hyperthermia was applied to murine dorsal skin 3 days prior to,concurrent with,or 2 days post sensitization with fluorescein isothiocyanate (FITC) in BALB/c mice.Local hyperthermia temperatures at 37℃,39℃,41℃ and 43℃ were applied to mouse dorsal skin and the severity of CHS was calculated by measuring the swelling response of the challenged ears.Results Local hyperthermia at 39℃,41℃ and 43℃ prior to sensitization reduced the severity of CHS,as compared with that at 37℃.The suppression of CHS was temperature dependant in that higher temperature had a stronger effect.On the contrary,the hyperthermia treatments,either concurrent with or post-sensitization,resulted in an enhanced temperature-dependant ear swelling response.Conclusions The severity of murine CHS could be influenced by local hyperthermia at the sensitization stage in a temperature dependant manner.The temporal effect of local hyperthermia suggested a novel factor in interpreting the severity of allergic contact dermatitis.

  4. Final Environmental Assessment Addressing Aerial Application of Herbicides at Joint Base Charleston-Weapons Station Charleston, South Carolina

    Science.gov (United States)

    2013-02-01

    redcockaded woodpecker (Picoides borealis), brown-headed nuthatch (Sitta pusilla), southern fox squirrel (Sciurus niger ), northern bobwhite (Colinus...supply west of the installation, and is saline near coastal waters (JB CHS-WS 2009). JB CHS-WS does not operate public supply wells because potable...species include the gray squirrel (Sciurus carolinensis), white-tailed deer (Odocoileus virginianus), fox squirrel (Sciurus niger ), eastern cottontail

  5. Delayed phagocytosis and bacterial killing in Chediak-Higashi syndrome neutrophils detected by a fluorochrome assay: ultrastructural aspects

    OpenAIRE

    Raquel Bellinati-Pires; Maristela M Salgado; Joazeiro, Paulo P.; Carneiro-Sampaio, Magda M. S.

    1992-01-01

    The few studies already published about phagocyte functions in Chediak-Higashi syndrome (CHS) has stated that neutrophils present slow rate of bacterial killing but normally ingest microorganisms. In the present study, both phagocytosis and killing of Staphylococcus aureus were verified to be in neutrophils from two patients with CHS when these functions were simultaneously evaluated by a fluorochrome phagocytosis assay. Electron microscopic examination showed morphologic differences among ne...

  6. Proceedings of US-Japan heliotron-stellarator workshop: Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    1987-01-01

    This paper is the first of four volumes on the US-Japan Heliotron-Stellarator workshop. It contains talks on the Heliotron E experiment, the compact helical system (CHS) program, the status of the ATF project, the status of the W VII-AS, the status of the TJ-II program, the ATF experimental plans, the ATF diagnostics, the compact Helical system, and the CHS experimental program and diagnostics. (LSP)

  7. SYNTHESIS AND PROPERTIES OF A NEW ASYMMETRIC TRIS(1,10-PHENANTHROLINE) RUTHENIUM( Ⅱ ) COMPLEX%不对称三邻菲咯啉钌(Ⅱ)配合物的合成及其性质

    Institute of Scientific and Technical Information of China (English)

    舒火明; 李华明; 王鹏; 唐作华

    2000-01-01

    A new compound,phen-NHCOCH=CHs2,and an ECL-active species,Ru ( Ⅱ )(phen)2 [phen-NHCOCH =CHs2] (PF6)2(phen=1,10-phenanthroline), have been designed and synthesized. Their compositions and structures were confirmed by elemental analysis,FT-IR,1HNMR and ESI-MS. Also,their properties of electrochemistry and fluorescence reported.

  8. Molecular evolution and sequence divergence of plant chalcone synthase and chalcone synthase-Like genes.

    Science.gov (United States)

    Han, Yingying; Zhao, Wenwen; Wang, Zhicui; Zhu, Jingying; Liu, Qisong

    2014-06-01

    Plant chalcone synthase (CHS) and CHS-Like (CHSL) proteins are polyketide synthases. In this study, we evaluated the molecular evolution of this gene family using representative types of CHSL genes, including stilbene synthase (STS), 2-pyrone synthase (2-PS), bibenzyl synthase (BBS), acridone synthase (ACS), biphenyl synthase (BIS), benzalacetone synthase, coumaroyl triacetic acid synthase (CTAS), and benzophenone synthase (BPS), along with their CHS homologs from the same species of both angiosperms and gymnosperms. A cDNA-based phylogeny indicated that CHSLs had diverse evolutionary patterns. STS, ACS, and 2-PS clustered with CHSs from the same species (late diverged pattern), while CTAS, BBS, BPS, and BIS were distant from their CHS homologs (early diverged pattern). The amino-acid phylogeny suggested that CHS and CHSL proteins formed clades according to enzyme function. The CHSs and CHSLs from Polygonaceae and Arachis had unique evolutionary histories. Synonymous mutation rates were lower in late diverged CHSLs than in early diverged ones, indicating that gene duplications occurred more recently in late diverged CHSLs than in early diverged ones. Relative rate tests proved that late diverged CHSLs had unequal rates to CHSs from the same species when using fatty acid synthase, which evolved from the common ancestor with the CHS superfamily, as the outgroup, while the early diverged lineages had equal rates. This indicated that late diverged CHSLs experienced more frequent mutation than early diverged CHSLs after gene duplication, allowing obtaining new functions in relatively short period of time.

  9. Eight International London Cough Symposium 2014: Cough hypersensitivity syndrome as the basis for chronic cough.

    Science.gov (United States)

    Chung, Kian Fan; Canning, Brendan; McGarvey, Lorcan

    2015-12-01

    At the Eighth International London Cough Conference held in London in July 2014, the focus was on the relatively novel concept of cough hypersensitivity syndrome (CHS) as forming the basis of chronic cough. This concept has been formulated following understanding of the neuronal pathways for cough and a realisation that not all chronic cough is usually associated with a cause. The CHS is defined by troublesome coughing triggered by low level of thermal, mechanical or chemical exposure. It also encompasses other symptoms or sensations such as laryngeal hypersensitivity, nasal hypersensitivity and possibly also symptoms related to gastrooesopahgeal reflux. The pathophysiologic basis of the CHS is now being increasingly linked to an enhancement of the afferent pathways of the cough reflex both at the peripheral and central levels. Mechanisms involved include the interactions of inflammatory mechanisms with cough sensors in the upper airways and with neuronal pathways of cough, associated with a central component. Tools for assessing CHS in the clinic need to be developed. New drugs may be developed to control CHS. A roadmap is suggested from the inception of the CHS concept towards the development of newer antitussives at the Symposium.

  10. Hurdle Effect of Antimicrobial Activity Achieved by Time Differential Releasing of Nisin and Chitosan Hydrolysates from Bacterial Cellulose.

    Science.gov (United States)

    Hsiao, Hui-Ling; Lin, Shih-Bin; Chen, Li-Chen; Chen, Hui-Huang

    2016-05-01

    We investigated the combined antimicrobial effect of nisin and chitosan hydrolysates (CHs) by regulating the antimicrobial reaction order of substances due to differential releasing rate from hydroxypropylmethylcellulose-modified bacterial cellulose (HBC). The minimum inhibitory concentration of nisin against Staphylococcus aureus and that of CHs against Escherichia coli were 6 IU and 200 μg/mL, respectively. Hurdle and additive effects in antimicrobial tests were observed when nisin was used 6 h before CH treatment against S. aureus; similar effects were observed when CH was used before nisin treatment against E. coli. Simultaneously combined treatment of nisin and CHs exhibited the low antimicrobial effect. HBC was then selected as the carrier for the controlled release of nisin and CHs. A 90% inhibition in the growth of S. aureus and E. coli was achieved when 30 IU-nisin-containing HBC and 62.5 μg/mL-CH-containing HBC were used simultaneously. The controlled release of nisin and CHs by using HBC minimized the interaction between nisin and CHs as well as increased the number of microbial targets.

  11. Child health care utilisation in families with young or single mothers in a Swedish county.

    Science.gov (United States)

    Wallby, Thomas; Modin, Bitte; Hjern, Anders

    2013-03-01

    Young age and lone parenthood are risk factors for impaired health among mothers and their children. Due to the higher risks of negative influences on physical and mental health, young and single mothers should be of special concern to the Child Health Services (CHS). In the present study, we investigated consumption patterns of child health care services among young and single mothers in Uppsala County, Sweden to study whether they are reached by the universal CHS program and if selective or indicative measures were administered in daily CHS practice. Register data on CHS contacts and socio-demographic indicators were collected for 10692 infants, born in 1998-2006. Results show small differences in contact pattern and immunization status, between children of young versus older, and single versus cohabiting mothers. However, both young (RR 0.64) and single (RR 0.80) mothers had significantly lower rates of participation in parental group. The CHS were consequently successful in implementing the universal preventive child health programme for all families, including families with young or single mothers. There was no indication, however, of an established selective preventive strategy aimed at these high risk families. Programs for strengthening the support provided to vulnerable families by the CHS are needed.

  12. Cannabinoid Hyperemesis Syndrome: Case Report of a Paradoxical Reaction with Heavy Marijuana Use

    Directory of Open Access Journals (Sweden)

    Benjamin Cox

    2012-01-01

    Full Text Available Cannabinoid hyperemesis syndrome (CHS is a rare constellation of clinical findings that includes a history of chronic heavy marijuana use, severe abdominal pain, unrelenting nausea, and intractable vomiting. A striking component of this history includes the use of hot showers or long baths that help to alleviate these symptoms. This is an underrecognized syndrome that can lead to expensive and unrevealing workups and can leave patients self-medicating their nausea and vomiting with the very substance that is causing their symptoms. Long-term treatment of CHS is abstinence from marijuana use—but the acute symptomatic treatment of CHS has been a struggle for many clinicians. Many standard medications used for the symptomatic treatment of CHS (including ondansetron, promethazine, and morphine have repeatedly been shown to be ineffective. Here we present the use of lorazepam as an agent that successfully and safely treats the tenacious symptoms of CHS. Additionally, we build upon existing hypotheses for the pathogenesis of CHS to try to explain why a substance that has been used for thousands of years is only now beginning to cause this paradoxical hyperemesis syndrome.

  13. Ingression Progression Complexes Control Extracellular Matrix Remodelling during Cytokinesis in Budding Yeast

    Science.gov (United States)

    Foltman, Magdalena; Molist, Iago; Arcones, Irene; Sacristan, Carlos; Filali-Mouncef, Yasmina; Roncero, Cesar; Sanchez-Diaz, Alberto

    2016-01-01

    Eukaryotic cells must coordinate contraction of the actomyosin ring at the division site together with ingression of the plasma membrane and remodelling of the extracellular matrix (ECM) to support cytokinesis, but the underlying mechanisms are still poorly understood. In eukaryotes, glycosyltransferases that synthesise ECM polysaccharides are emerging as key factors during cytokinesis. The budding yeast chitin synthase Chs2 makes the primary septum, a special layer of the ECM, which is an essential process during cell division. Here we isolated a group of actomyosin ring components that form complexes together with Chs2 at the cleavage site at the end of the cell cycle, which we named ‘ingression progression complexes’ (IPCs). In addition to type II myosin, the IQGAP protein Iqg1 and Chs2, IPCs contain the F-BAR protein Hof1, and the cytokinesis regulators Inn1 and Cyk3. We describe the molecular mechanism by which chitin synthase is activated by direct association of the C2 domain of Inn1, and the transglutaminase-like domain of Cyk3, with the catalytic domain of Chs2. We used an experimental system to find a previously unanticipated role for the C-terminus of Inn1 in preventing the untimely activation of Chs2 at the cleavage site until Cyk3 releases the block on Chs2 activity during late mitosis. These findings support a model for the co-ordinated regulation of cell division in budding yeast, in which IPCs play a central role. PMID:26891268

  14. A new financial budgetary system for community health services institutions in China.

    Science.gov (United States)

    You, Chuanmei; Yao, Lan; Fu, Jiakang; Wang, Fang; Wang, Hongqing; Dai, Tao

    2011-01-01

    The separation of revenue and expenditure budgets (SREB) is a new financial budgetary system that is being implemented in community health services (CHS) institutions in some areas in China. Through literature review, it was found that, derived from the traditional separation of revenue and expenditure budgets (TSREB) implemented in administrative public services units, SREB and TSREB have something in common and yet many more differences. On the basis of some quantitative and qualitative data that were collected by field survey, it was also found that implementation of SREB in CHS institutions brings positive outcomes in terms of the quantity, quality and efficiency of services; residents' satisfaction; and the behavior of CHS institutions. The conclusion can be suggested that SREB, as a system having impact upon the incentives facing CHS institutions and the nature of governmental responsibility for developing CHS in China, will promote CHS institutions to fulfill basic service functions if implemented well. Therefore, it is a system that is worth further development and evaluation.

  15. Interleukin-12 reverses the inhibitory impact of photodynamic therapy (PDT) on the murine contact hypersensitivity response

    Science.gov (United States)

    Simkin, Guillermo O.; Levy, Julia G.; Hunt, David W. C.

    1998-05-01

    Treatment of mice with certain photosensitizers combined with exposure to visible light limits the development of the immunologically-mediated contact hypersensitivity (CHS) response against topically-applied chemical haptens. Understanding of the inhibitory action of photosensitizers upon the CHS response is incomplete. Benzoporphyrin derivative monoacid ring A (BPD-MA, verteporfin), a photosensitizer with immunomodulatory activity, strongly depressed CHS responses to the hapten dinitrofluorobenzene (DNFB). However, if mice were administered 1 (mu) g of a recombinant preparation of the pro- inflammatory cytokine interleukin-12 (rIL-12), full-fledged CHS responses to DNFB ensued in animals treated with BPD-MA and light. In contrast, when rIL-12 was given in combination with an anti-IL-12 antibody the restorative effect of rIL-12 on the CHS response of PDT-treated mice was blocked. Evaluation of the cytokine status of spleen and draining lymph node cells showed for DNFB painted animals, that the release of the immunosuppressive cytokine IL-10 was increased by PDT and rIL-12 counter-acted the increase in IL-10 liberation associated with PDT. These studies indicate that IL-10 formation is upregulated and the availability of IL-12 may be limited in mice treated with PDT. These features may contribute to deficient CHS responses observed with PDT.

  16. Ingression Progression Complexes Control Extracellular Matrix Remodelling during Cytokinesis in Budding Yeast.

    Directory of Open Access Journals (Sweden)

    Magdalena Foltman

    2016-02-01

    Full Text Available Eukaryotic cells must coordinate contraction of the actomyosin ring at the division site together with ingression of the plasma membrane and remodelling of the extracellular matrix (ECM to support cytokinesis, but the underlying mechanisms are still poorly understood. In eukaryotes, glycosyltransferases that synthesise ECM polysaccharides are emerging as key factors during cytokinesis. The budding yeast chitin synthase Chs2 makes the primary septum, a special layer of the ECM, which is an essential process during cell division. Here we isolated a group of actomyosin ring components that form complexes together with Chs2 at the cleavage site at the end of the cell cycle, which we named 'ingression progression complexes' (IPCs. In addition to type II myosin, the IQGAP protein Iqg1 and Chs2, IPCs contain the F-BAR protein Hof1, and the cytokinesis regulators Inn1 and Cyk3. We describe the molecular mechanism by which chitin synthase is activated by direct association of the C2 domain of Inn1, and the transglutaminase-like domain of Cyk3, with the catalytic domain of Chs2. We used an experimental system to find a previously unanticipated role for the C-terminus of Inn1 in preventing the untimely activation of Chs2 at the cleavage site until Cyk3 releases the block on Chs2 activity during late mitosis. These findings support a model for the co-ordinated regulation of cell division in budding yeast, in which IPCs play a central role.

  17. A Gut Gone to Pot: A Case of Cannabinoid Hyperemesis Syndrome due to K2, a Synthetic Cannabinoid

    Directory of Open Access Journals (Sweden)

    Anene Ukaigwe

    2014-01-01

    Full Text Available Cannabinoid Hyperemesis Syndrome (CHS was first described in 2004. Due to its novelty, CHS is often unrecognized by clinicians leading to expensive workup of these patients with cyclical symptoms. It may take up to 9 years to diagnose CHS. CHS is characterized by cyclical nausea and vomiting, abdominal pain, and an unusual compulsion to take hot showers in the presence of chronic use of cannabinoids. Cannabicyclohexanol is a synthetic cannabinoid, popularly known as K2 spice. It is a popular marijuana alternative among teenagers and young adults since it is readily available as herbal incense. Unlike marijuana, many users know that K2 is not detected in conventional urine drug screens, allowing those users to conceal their intake from typical detection methods. Serum or urine gas chromatography mass spectrophotometry is diagnostic, though not widely available. Thus, it is imperative for clinicians to recognize CHS, even with negative UDS, to provide cost-effective care. We present a 38-year-old man with a 10-year history of cannabis, and 1-year history of K2 abuse admitted with 1-week history of episodes of nausea, vomiting of clear fluids, and epigastric discomfort. Symptoms are relieved only by hot showers. Extensive laboratory, radiologic, and endoscopic evaluation was unrevealing. CHS was diagnosed, based on proposed criteria by Simonetti et al.

  18. Isolation of culturable microorganisms from free-ranging bottlenose dolphins (Tursiops truncatus) from the southeastern United States.

    Science.gov (United States)

    Morris, Pamela J; Johnson, Wesley R; Pisani, John; Bossart, Gregory D; Adams, Jeff; Reif, John S; Fair, Patricia A

    2011-03-24

    Reports of diseases in marine mammals are increasing worldwide, however our understanding of the microorganisms associated with marine mammals is still limited. In this study, we cultured bacteria and fungi isolated from the upper respiratory tract (blowhole), gastric fluid and anus of 180 wild bottlenose dolphins (Tursiops truncatus) from two estuarine locations along the southeastern Atlantic Coast of the United States. A total of 339 and 491 isolates from Charleston, SC (CHS) and Indian River Lagoon, FL (IRL) dolphins, respectively, were cultured from gastric (70 CHS/82 IRL), fecal (141 CHS/184 IRL), and blowhole (128 CHS/225 IRL) swabs on selective media used for routine clinical microorganisms of human concern. The most frequently cultured Gram-negative bacteria from all sample and study types were Plesiomonas shigelloides, Aeromonas hydrophila, Escherichia coli, and Pseudomonas fluorescens. Among the Gram-positive bacteria, Clostridium perfringens, Bacillus sp., and Staphylococcus Coag. Neg were the predominant organisms. For fungi, the most abundant species were Candida glabrata, budding yeasts, and Candida tropicalis. Of concern, the MRSA strain of Staphylococcus aureus was detected in the blowhole and gastric swabs from CHS dolphins. In general, a greater prevalence of bacteria and fungi (four-fold increase) were cultured from IRL than CHS animals. Together, these culture-dependent studies, coupled to on-going culture-independent approaches, should help establish a baseline of microorganisms associated with bottlenose dolphins and aid in the identification of organisms responsible for infectious diseases(s) in these animals.

  19. 社区卫生服务举办主体多元化与政府主导权被迫让渡的关系%Multi-ownership of community health services and the transferred govrenment leadership in China

    Institute of Scientific and Technical Information of China (English)

    张明吉; 王伟; 李静然; 杨蓉蓉; 徐玲; 严非

    2016-01-01

    Objective:About half of Community Health Services ( CHS) in China are not government-owned, forming a multi-ownership situation of CHS .This study aims to examine the effect of “multi-ownership policy” on the development of CHS and put forward suggestions for improving the governance of CHS .Methods:We applied maxi-mum variation sampling to select health workers of different CHS specialties and administrators from local health bu -reaus in District T of Shaanxi province and District X of Shandong province .Inductive thematic analysis was utilized to interpret the development and ramification of multi-ownership policy , and then to formulate substantive theory .Re-sults:The adoption of multi-ownership in two districts was an adaptive strategy based on the limited public finance , institutional restriction of government , and diversity of health resources .This policy promoted the establishment of CHS network , and meanwhile the government's leadership of CHS development was transferred to multiple owners of CHS, which caused the lack of CHS autonomy , fragmented regulation power and deviated development of CHS .Con-clusions:The key problem of multi-ownership situation in CHS development is the problematic partition of governance power.In order to redeem the leadership of CHS development to the local government , it is necessary to strengthen service regulation while to decentralize the facility management power to CHS , to adjust service price ,and to deepen medical insurance coverage of CHS to enhance autonomy of CHS .%目的:社区卫生服务机构中约有半数不属于政府直接举办,形成了多元化的举办主体格局。本文用定性研究的方法审视“举办主体多元化”对社区卫生发展的影响,并探讨监管权解决方案。方法:在陕西省T区和山东省X区,用最大差异抽样法选取不同专业的卫生技术人员以及卫生行政人员进行访谈。采用归纳式的主题分析方法,解释举办主

  20. Starter substrate specificities of wild-type and mutant polyketide synthases from Rutaceae.

    Science.gov (United States)

    Lukacin, Richard; Schreiner, Stephan; Silber, Katrin; Matern, Ulrich

    2005-02-01

    Chalcone synthases (CHSs) and acridone synthases (ACSs) belong to the superfamily of type III polyketide synthases (PKSs) and condense the starter substrate 4-coumaroyl-CoA or N-methylanthraniloyl-CoA with three malonyl-CoAs to produce flavonoids and acridone alkaloids, respectively. ACSs which have been cloned exclusively from Ruta graveolens share about 75-85% polypeptide sequence homology with CHSs from other plant families, while 90% similarity was observed with CHSs from Rutaceae, i.e., R. graveolens, Citrus sinensis and Dictamnus albus. CHSs cloned from many plants do not accept N-methylanthraniloyl-CoA as a starter substrate, whereas ACSs were shown to possess some side activity with 4-coumaroyl-CoA. The transformation of an ACS to a functional CHS with 10% residual ACS activity was accomplished previously by substitution of three amino acids through the corresponding residues from Ruta-CHS1 (Ser132Thr, Ala133Ser and Val265Phe). Therefore, the reverse triple mutation of Ruta-CHS1 (mutant R2) was generated, which affected only insignificantly the CHS activity and did not confer ACS activity. However, competitive inhibition of CHS activity by N-methylanthraniloyl-CoA was observed for the mutant in contrast to wild-type CHSs. Homology modeling of ACS2 with docking of 1,3-dihydroxy-N-methylacridone suggested that the starter substrates for CHS or ACS reaction are placed in different topographies in the active site pocket. Additional site specific substitutions (Asp205Pro/Thr206Asp/His207Ala or Arg60Thr and Val100Ala/Gly218Ala, respectively) diminished the CHS activity to 75-50% of the wild-type CHS1 without promoting ACS activity. The results suggest that conformational changes in the periphery beyond the active site cavity volumes determine the product formation by ACSs vs. CHSs in R. graveolens. It is likely that ACS has evolved from CHS, but the sole enlargement of the active site pocket as in CHS1 mutant R2 is insufficient to explain this process.

  1. Effective photosensitization-based inactivation of Gram (-) food pathogens and molds using the chlorophyllin-chitosan complex: towards photoactive edible coatings to preserve strawberries.

    Science.gov (United States)

    Buchovec, Irina; Lukseviciute, Viktorija; Marsalka, Arunas; Reklaitis, Ignas; Luksiene, Zivile

    2016-04-01

    This study is focused on the novel approaches to enhance the inactivation of the Gram (-) food pathogen Salmonella enterica and harmful molds in vitro and on the surface of strawberries using the chlorophyllin-chitosan complex. Salmonella enterica (∼1 × 10(7) CFU mL(-1)) was incubated with chlorophyllin 1.5 × 10(-5) M (Chl, food additive), chitosan 0.1% (CHS, food supplement) or the chlorophyllin-chitosan complex (1.5 × 10(-5) M Chl-0.1% CHS) and illuminated with visible light (λ = 405 nm, light dose 38 J cm(-2)) in vitro. Chlorophyllin (Chl)-based photosensitization inactivated Salmonella just by 1.8 log. Chitosan (CHS) alone incubated for 2 h with Salmonella reduced viability 2.15 log, whereas photoactivated Chl-CHS diminished bacterial viability by 7 log. SEM images indicate that the Chl-CHS complex under these experimental conditions covered the entire bacterial surface. Significant cell membrane disintegration was the main lethal injury induced in Gram (-) bacteria by this treatment. Analysis of strawberry decontamination from surface-inoculated Salmonella indicated that photoactivated Chl-CHS (1.5 × 10(-5) M Chl-0.1% CHS, 30 min incubation, light dose 38 J cm(-2)) coatings diminished the pathogen population on the surface of strawberries by 2.2 log. Decontamination of strawberries from naturally distributed yeasts/molds revealed that chitosan alone reduced the population of yeasts/molds just by 0.4 log, Chl-based photosensitization just by 0.9 log, whereas photoactivated Chl-CHS coatings reduced yeasts/molds on the surface of strawberries by 1.4 log. Electron paramagnetic resonance spectroscopy confirmed that no additional photosensitization-induced free radicals have been found in the strawberry matrix. Visual quality (color, texture) of the treated strawberries was not affected either. In conclusion, photoactive Chl-CHS exhibited strong antimicrobial action against more resistant to photosensitization Gram (-) Salmonella enterica in comparison with

  2. Contaminant blubber burdens in Atlantic bottlenose dolphins (Tursiops truncatus) from two southeastern US estuarine areas: concentrations and patterns of PCBs, pesticides, PBDEs, PFCs, and PAHs.

    Science.gov (United States)

    Fair, Patricia A; Adams, Jeff; Mitchum, Gregory; Hulsey, Thomas C; Reif, John S; Houde, Magali; Muir, Derek; Wirth, Ed; Wetzel, Dana; Zolman, Eric; McFee, Wayne; Bossart, Gregory D

    2010-03-01

    Polychlorinated biphenyls (PCBs), chlorinated pesticides (i.e., dichlorodiphenyltrichloroethane (DDT) and its metabolites, chlordanes (CHLs), dieldrin, hexachlorobenzene (HCB), and mirex), polybrominated diphenyl ethers (PBDEs), perfluorinated chemicals (PFCs), and polyaromatic hydrocarbons (PAHs) were measured in blubber biopsy samples collected from 139 wild bottlenose dolphins (Tursiops truncatus) during 2003-2005 in Charleston (CHS), SC and the Indian River Lagoon (IRL), FL. Dolphins accumulated a similar suite of contaminants with summation operatorPCB dominating (CHS 64%, IRL 72%), followed by summation operatorDDT (CHS 20%, IRL 17%), summation operatorCHLs (CHS 7%; IRL 7%), summation operatorPBDE (CHS 4%, IRL 2%), PAH at 2%, and dieldrin, PFCs and mirex each 1% or less. Together summation operatorPCB and summation operatorDDT concentrations contributed approximately 87% of the total POCs measured in blubber of adult males. summation operatorPCBs in adult male dolphins exceed the established PCB threshold of 17mg/kg by a 5-fold order of magnitude with a 15-fold increase for many animals; 88% of the dolphins exceed this threshold. For male dolphins, CHS (93,980ng/g lipid) had a higher summation operatorPCBs geomean compared to the IRL (79,752ng/g lipid) although not statistically different. In adult males, the PBDE geometric mean concentration was significantly higher in CHS (5920ng/g lipid) than the IRL (1487ng/g). Blubber summation operatorPFCs concentrations were significantly higher in CHS dolphins. In addition to differences in concentration of PCB congeners, summation operatorPBDE, TEQ, summation operatorCHLs, mirex, dieldrin, and the ratios summation operatorDDE/ summation operatorDDT and trans-nonachlor/cis-nonachlor were the most informative for discriminating contaminant loads in these two dolphin populations. Collectively, the current summation operatorPCB, summation operatorDDT, and summation operatorPBDEs blubber concentrations found in CHS

  3. 冕洞内矢量磁场的分布和演化%Distribution and Evolution of Vector Magnetic Fields in Coronal Holes

    Institute of Scientific and Technical Information of China (English)

    杨书红

    2012-01-01

    With the observations from the SOHO, Hinode, STEREO, and SDO, we investigate some aspects of CHs in detail for the first time, such as the evolution of vector magnetic field and magnetic nonpotentiality in CHs, and obtain a series of results. (1) Response of the solar atmosphere to the magnetic field distribution and evolution in a CH. We study the magnetic fields in a CH and at the CH boundary, and present the corresponding atmospheric response of different overlying layers to the magnetic field distribution and evolution. We also quantitatively analyze the relationship between the magnetic flux density and atmospheric emissions at different wavelengths. (2) Evolution of dipoles in an equatorial CH and its effect on the decay of the CH. We investigate the evolution of dipolar magnetic fields in an equatorial CH region. In the CH, the submergence of initial loops after their emergence is observed for the first time. The area where the dipoles are located becomes a place of mixed polarities instead of the unipolar fields, resulting in the change of the overlying corona from a CH area to a quiet region. (3) Distribution of vector magnetic fields and magnetic nonpotentiality of CHs. We investigate the vector magnetic fields, current densities, and current helicities in two CHs, and compare them with two quiet regions. We find that: (i) in the areas where the large current helicities are concentrated, there are strong vertical and horizontal field elements; (ii) the mean current density in the magnetic flux concentrations with the vertical fields stronger than 100 Gs is as large as (0.012 0.001) Am-2, consistent with that in the flare productive active regions; (iii) the magnetic fields in both the CHs and the quiet regions are nonpotential. (4) SDO observations of magnetic reconnection at CH boundaries. At the CH boundaries, we find many coronal jets as the signatures of magnetic reconnection, below which the magnetic emergence and cancellation are observed. We study

  4. Determination and validation of chikusetsusaponin IVa in rat plasma by UPLC-MS/MS and its application to pharmacokinetic study.

    Science.gov (United States)

    Wang, Ying; Liu, Shi-Ping; Guo, Mei-Hua; Wang, Zhuo

    2016-09-01

    A novel, sensitive and rapid ultra-performance liquid chromatography-tandem mass spectrometric method for the quantification of chikusetsusaponin IVa (CHS-IVa) in rat plasma was established and validated. Plasma samples were pre-treated by precipitation of protein with acetonitrile and chromatographed on a Waters Symmetry C18 analytical column (4.6 × 50 mm, i.d., 3.5 μm) using a mobile phase consisting of methanol and water containing 0.05% formic acid (55:45, v/v) at a flow rate of 0.4 mL/min. The deprotonated molecular ions [M - H](-) were employed in electrospray negative ionization mode and selected reaction monitoring transitions were performed for detection. The calibration curves exhibited good linearity (r > 0.99) over the range of 0.5-1000 ng/mL for CHS-IVa. The recoveries of CHS-IVa were >92.5% and exhibited no severe matrix effect. This method was successfully applied in the pharmacokinetic study of CHS-IVa in rats. For oral administration, the plasma concentrations of CHS-IVa increased to a peak value at 0.35 ± 0.14 h, followed by a gradual decrease to the lower limit of quantitation in 24 h. For intravenous administration, the plasma concentrations of CHS-IVa decreased quickly (t1/2 , 1.59 ± 0.25 h). The absolute bioavailability of CHS-IVa in rats was 8.63%. Copyright © 2016 John Wiley & Sons, Ltd.

  5. Prostaglandin E2 Promotes UV Radiation-Induced Immune Suppression through DNA Hypermethylation

    Directory of Open Access Journals (Sweden)

    Ram Prasad

    2013-07-01

    Full Text Available Exposure of mice to UV radiation results in suppression of the contact hypersensitivity (CHS response. Here, we report that the UV-induced suppression of CHS is associated with increases in the levels of cyclooxygenase-2 (COX-2, prostaglandin E2 (PGE2, and PGE2 receptors in the exposed skin. UV radiation.induced suppression of CHS was inhibited by topical treatment of the skin with celecoxib or indomethacin (inhibitors of COX-2 or AH6809 (an EP2 antagonist. Moreover, mice deficient in COX-2 were found to be resistant to UV-induced suppression of CHS. The exposure of wild-typemice to UVB radiation resulted in DNA hypermethylation, increased DNA methyltransferase (Dnmt activity, and elevated levels of Dnmt1, Dnmt3a, and Dnmt3b proteins in the skin, and these responses were downregulated on topical treatment of the site of exposure after irradiation with indomethacin or EP2 antagonist. Topical treatment of UVB-exposed COX-2.deficient mice with PGE2 enhanced the UVB-induced suppression of CHS as well as global DNA methylation and elevated the levels of Dnmt activity and Dnmt proteins in the skin. Intraperitoneal injection of 5-aza-2′-deoxycytidine (5-Aza-dc, a DNA demethylating agent, restored the CHS response to 2,4-dinitrofluorobenzene in UVB-exposed skin and this was associated with the reduction in global DNA methylation and Dnmt activity and reduced levels of Dnmt proteins. Furthermore, treatment with 5-Aza-dc reversed the effect of PGE2 on UV-induced suppression of CHS in COX-2.deficient mice. These findings reveal a previously unrecognized role for PGE2 in the promotion of UVB-induced immunosuppression and indicate that it is mediated through PGE2 regulation of DNA methylation.

  6. Acylphloroglucinol Biosynthesis in Strawberry Fruit.

    Science.gov (United States)

    Song, Chuankui; Ring, Ludwig; Hoffmann, Thomas; Huang, Fong-Chin; Slovin, Janet; Schwab, Wilfried

    2015-11-01

    Phenolics have health-promoting properties and are a major group of metabolites in fruit crops. Through reverse genetic analysis of the functions of four ripening-related genes in the octoploid strawberry (Fragaria × ananassa), we discovered four acylphloroglucinol (APG)-glucosides as native Fragaria spp. fruit metabolites whose levels were differently regulated in the transgenic fruits. The biosynthesis of the APG aglycones was investigated by examination of the enzymatic properties of three recombinant Fragaria vesca chalcone synthase (FvCHS) proteins. CHS is involved in anthocyanin biosynthesis during ripening. The F. vesca enzymes readily catalyzed the condensation of two intermediates in branched-chain amino acid metabolism, isovaleryl-Coenzyme A (CoA) and isobutyryl-CoA, with three molecules of malonyl-CoA to form phlorisovalerophenone and phlorisobutyrophenone, respectively, and formed naringenin chalcone when 4-coumaroyl-CoA was used as starter molecule. Isovaleryl-CoA was the preferred starter substrate of FvCHS2-1. Suppression of CHS activity in both transient and stable CHS-silenced fruit resulted in a substantial decrease of APG glucosides and anthocyanins and enhanced levels of volatiles derived from branched-chain amino acids. The proposed APG pathway was confirmed by feeding isotopically labeled amino acids. Thus, Fragaria spp. plants have the capacity to synthesize pharmaceutically important APGs using dual functional CHS/(phloriso)valerophenone synthases that are expressed during fruit ripening. Duplication and adaptive evolution of CHS is the most probable scenario and might be generally applicable to other plants. The results highlight that important promiscuous gene function may be missed when annotation relies solely on in silico analysis.

  7. 蓝粒小麦花青素合成途径中的查尔酮合酶基因和类黄酮3′5′-羟化酶基因3′末端的克隆和表达分析%Cloning and Expression of Two Chalcone Synthase and a Flavonoid 3′5′- Hydroxylase 3′-end cDNAs from Developing Seeds of Blue-grained Wheat Involved in Anthocyanin Biosynthetic Pathway

    Institute of Scientific and Technical Information of China (English)

    杨国华; 李滨; 高建伟; 刘建中; 赵学强; 郑琪; 童依平; 李振声

    2004-01-01

    采用RT-PCR和RACE方法从蓝粒小麦(Triticum aestivum L.×Thinopyrum ponticum(Podp.)Z.W.Liu et R.R.-C. Wang)发育种子中克隆到两个查尔酮合酶基因(TaCHS.t1,TaCHS.w1),分别编码394个氨基酸,二者核苷酸和氨基酸序列的同源性分别为96.0%和98.9%;而且克隆到一个类黄酮3′5′-羟化酶基因(F3′5′H)3′-末端.分别从长穗偃麦草(Thinopyrum ponticum(Podp.)Z.W.Liu et R.R.-C.Wang)、蓝粒小麦、白粒小麦和中国春基因组中分离到查尔酮合酶基因(CHS)的全长序列(ThpCHS.tg,TaCHS.bg,TaCHS.wg,TaCHS.csg),它们的核苷酸序列之间具有很高的同源性,且均含有一个内含子(intron),序列差异主要在内含子.通过DNA序列比较,发现一个CHS与亲本之一的长穗偃麦草基因组同源性达100%,一个CHS与另一白粒亲本基因组同源性达99%,表明来自于父、母本的CHS在蓝粒发育的种子中均表达.Southern杂交结果表明CHS在小麦中的拷贝数至少有4个,不同颜色的蓝粒小麦、白粒小麦间拷贝数基本一致,但都与长穗偃麦草有差异,根据以上结果初步判定CHS在蓝粒小麦中属于一个多基因家族.Reverse Northern分析表明CHS在开花后15 d发育的蓝粒小麦中具有较强的表达;花后21 d F3′5′H和DFR的转录本积累显著高于CHS,基本上检测不到CHS的表达.这三个基因在蓝粒小麦中的表达顺序与其他植物花青素合成途径中的基因表达次序一致:CHS先于F3′5′H,F3′5′H先于DFR.实验还表明F3′5′H和DFR在幼叶中表达也较强,CHS仅在发育种子中表达,具有组织特异性.花后21 d,F3′5′H在白粒、蓝粒小麦和中国春种子中均强烈表达,蓝粒小麦CHS和DFR转录本比白粒小麦多.因此,认为在蓝粒小麦中存在花青素生物合成途径,在蓝色色素形成过程中,该途径中的结构基因受到调节基因的调控.%Using reverse transcription-polymerase chain reaction (RT-PGR) and rapid amplification of c

  8. 邯郸市社区卫生服务人员工作满意度调查%STUDY ON THE JOB SATISFACTION AND INFLUENCING FACTORS OF COMMUNITY HEALTH SERVICE PERSONNEL IN HANDAN

    Institute of Scientific and Technical Information of China (English)

    刘凤丽; 李焰; 张婷; 毛希瑞; 党瑞英; 焦丽艳

    2011-01-01

    [目的]研究邯郸市社区卫生服务(Community Health Service,CHS)机构工作人员满意度现况,探索满意度影响因素,为制定促进人员工作满意度相关策略提供依据.[方法]运用现状调查方法,进行现场问卷调查.收集邯郸市103个社区卫生服务机构(中心25个、站78个)工作人员工作满意度的相关数据,进行统计学分析. [结果]邯郸市社区卫生服务机构医务人员工作总的满意程度较高,学历、机构类型等是卫技人员工作满意度的影响因素.[结论]邯郸市CHS人员工作满意度受多方面因素影响,满意度有待进一步提高.%[Objective] To study the status of job satisfaction of Community Health Service (CHS) agencies in Handan, and explore the influencing factors of job satisfaction and provide suggestion on policy decision of improving the job satisfaction. [Methods] Investigation method and on-site survey was conducted to collect data about job satisfaction of the 103 (25 centers, 78 stations) Community Health Service (CHS) agencies in Handan. [Results] The total job satisfaction of the status of Community Health Service (CHS) agencies in Handan was higher at some extent. Age, education background and institution style were potential influencing factors of job satisfaction to CHS technical personal. [Conclusion] The job satisfaction of CHS personnel is influenced by intricate factors, and the job satisfaction of CHS personal should be improved in Handan.

  9. In vitro evaluation of the compatibility of a novel collagen-heparan sulfate biological scaffold with olfactory ensheathing cells

    Institute of Scientific and Technical Information of China (English)

    TANG Zhou-ping; YANG Jie; PAN Deng-ji; LIU Na; LI Zai-wang; XIE Xue-wei; CHEN Yun; SHI Yuan-hong; ZENG Wen-gao; WANG Shu-xin; CHEN Juan

    2010-01-01

    Background Stroke and traumatic injury to the nerve system may trigger axonal destruction and the formation of scar tissue, cystic cavitations and physical gaps.Olfactory ensheathing cells (OECs) can secrete neurotrophic factors to promote neurite growth and thus act as a prime candidate for autologous transplantation.Biological scaffolds can provide a robust delivery vehicle to injured nerve tissue for neural cell transplantation strategies, owing to the porous three-dimensional structures (3D).So transplantation of the purposeful cells seeded scaffolds may be a promising method for nerve tissue repair.This study aimed to evaluate the compatibility of a novel collagen-heparan sulfate biological scaffold with olfactory ensheathing cells in vitro.Methods Collagen-heparan sulfate (CHS) biological scaffolds were made, and then the scaffolds and OECs were co-cultured in vitro.The viability of OECs was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay at days 1, 3, 5 and 7.Statistical analysis was evaluated by student's t test.Significance was accepted at P <0.05.OECs were labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE), and the CFSE-labeled OECs were seeded into CHS scaffolds.The attachment and growth of OECs in CHS scaffolds were observed and traced directly by fluorescent microscopy and environmental scanning electron microscope (ESEM).Results CHS biological scaffolds had steady porous 3D structures and no cytotoxicity to OECs (F=0.14, P=0.9330).CHS biological scaffolds were good bridging materials for OECs attachment and proliferation, and they promoted the axonal growth.Conclusion The compatibility of CHS biological scaffolds with OECs is pretty good and CHS biological scaffold is a promising cell carrier for the implantation of OECs in nerve tissue bioengineering.

  10. Biochemical characterization of chitin synthase activity and inhibition in the African malaria mosquito, Anopheles gambiae

    Institute of Scientific and Technical Information of China (English)

    Xin Zhang; Kun Yan Zhu

    2013-01-01

    Chitin synthase (CHS) is an important enzyme catalyzing the formation of chitin polymers in all chitin containing organisms and a potential target site for insect pest control.However,our understanding of biochemical properties of insect CHSs has been very limited.We here report enzymatic and inhibitory properties of CHS prepared from the African malaria mosquito,Anopheles gambiae.Our study,which represents the first time to use a nonradioactive method to assay CHS activity in an insect species,determined the optimal conditions for measuring the enzyme activity,including pH,temperature,and concentrations of the substrate uridine diphosphate N-acetyl-D-glucosamine (UDPGlcNAc) and Mg++.The optimal pH was about 6.5-7.0,and the highest activity was detected at temperatures between 37℃ and 44℃.Dithithreitol is required to prevent melanization of the enzyme extract.CHS activity was enhanced at low concentration of GlcNAc,but inhibited at high concentrations.Proteolytic activation of the activity is significant both in the 500×g supernatant and the 40 000×g pellet.Our study revealed only slight in vitro inhibition ofA.gambiae CHS activity by diflubenzuron and nikkomycin Z at the highest concentration (2.5μmol/L) examined.There was no in vitro inhibition by polyoxin D at any concentration examined.Furthermore,we did not observe any in vivo inhibition of CHS activity by any of these chemicals at any concentration examined.Our results suggest that the inhibition of chitin synthesis by these chemicals is not due to direct inhibition of CHS in A.gambiae.

  11. Community hospitals – the place of local service provision in a modernising NHS: an integrative thematic literature review

    Directory of Open Access Journals (Sweden)

    Heaney David

    2006-12-01

    Full Text Available Abstract Background Recent developments within the United Kingdom's (UK health care system have re-awakened interest in community hospitals (CHs and their role in the provision of health care. This integrative literature review sought to identify and assess the current evidence base for CHs. Methods A range of electronic reference databases were searched from January 1984 to either December 2004 or February 2005: Medline, Embase, Web of Knowledge, BNI, CINAHL, HMIC, ASSIA, PsychInfo, SIGLE, Dissertation Abstracts, Cochrane Library, Kings Fund website, using both keywords and text words. Thematic analysis identified recurrent themes across the literature; narrative analyses were written for each theme, identifying unifying concepts and discrepant issues. Results The search strategy identified over 16,000 international references. We included papers of any study design focussing on hospitals in which care was led principally by general practitioners or nurses. Papers from developing countries were excluded. A review of titles revealed 641 potentially relevant references; abstract appraisal identified 161 references for review. During data extraction, a further 48 papers were excluded, leaving 113 papers in the final review. The most common methodological approaches were cross-sectional/descriptive studies, commentaries and expert opinion. There were few experimental studies, systematic reviews, economic studies or studies that reported on longer-term outcomes. The key themes identified were origin and location of CHs; their place in the continuum of care; services provided; effectiveness, efficiency and equity of CHs; and views of patients and staff. In general, there was a lack of robust evidence for the role of CHs, which is partly due to the ad hoc nature of their development and lack of clear strategic vision for their future. Evidence for the effectiveness and efficiency of the services provided was limited. Most people admitted to CHs

  12. Chikusetsu Saponin IVa Ameliorates Cerebral Ischemia Reperfusion Injury in Diabetic Mice via Adiponectin-Mediated AMPK/GSK-3β Pathway In Vivo and In Vitro.

    Science.gov (United States)

    Duan, Jialin; Yin, Ying; Cui, Jia; Yan, Jiajia; Zhu, Yanrong; Guan, Yue; Wei, Guo; Weng, Yan; Wu, Xiaoxiao; Guo, Chao; Wang, Yanhua; Xi, Miaomiao; Wen, Aidong

    2016-01-01

    Diabetes mellitus substantially increases the risk of stroke and enhances brain's vulnerability to ischemia insult. In a previous study, Chikusetsu saponin IVa (CHS) pretreatment was proved to protect the brain from cerebral ischemic in normal stroke models. Whether CHS could attenuate cerebral ischemia/reperfusion (I/R) injury in diabetic mice and the possible underlying mechanism are still unrevealed. Male C57BL/6 mice were injected streptozotocin to induce diabetes. After that, the mice were pretreated with CHS for 1 month, and then, focal cerebral ischemia was induced following 24-h reperfusion. The neurobehavioral scores, infarction volumes, and some cytokines in the brain were measured. Apoptosis was analyzed by caspase-3, Bax, and Bcl-2 expression. Downstream molecules of adiponectin (APN) were investigated by Western blotting. The results showed that CHS reduced infarct size, improved neurological outcomes, and inhibited cell injury after I/R. In addition, CHS pretreatment increased APN level and enhanced neuronal AdipoR1, adenosine monophosphate-activated protein kinase (AMPK), and glycogen synthase kinase 3 beta (GSK-3β) expression in a concentration-dependent manner in diabetic mice, and these effects were abolished by APN knockout (KO). In vitro test, CHS treatment also alleviated PC12 cell injury and apoptosis, evidenced by reduced tumor necrosis factor alpha (TNF-α), malondialdehyde (MDA) and caspase-3 expression, and Bax/Bcl-2 ratio in I/R injured cells. Moreover, CHS enhanced AdipoR1, AMPK, and GSK-3β expression in a concentration-dependent manner. Likewise, short interfering RNA (sinRNA) knockdown of liver kinase B1 (LKB1), an upstream kinase of AMPK, reduced the ability of CHS in protecting cells from I/R injury. Furthermore, this LKB1-dependent cellular protection resulted from AdipoR1 and APN activation, as supported by the experiment using sinRNA knockdown of AdipoR1 and APN. Thus, CHS protected brain I/R in diabetes through AMPK

  13. Comparative effects of α2δ-1 ligands in mouse models of colonic hypersensitivity

    Science.gov (United States)

    Meleine, Mathieu; Boudieu, Ludivine; Gelot, Agathe; Muller, Emilie; Lashermes, Amandine; Matricon, Julien; Silberberg, Celine; Theodorou, Vassilia; Eschalier, Alain; Ardid, Denis; Carvalho, Frederic A

    2016-01-01

    AIM To investigate anti-hypersensitive effects of α2δ-1 ligands in non-inflammatory and inflammation-associated colonic hypersensitivity (CHS) mouse models. METHODS To induce an inflammation-associated CHS, 1% dextran sulfate sodium (DSS) was administered to C57Bl/6J male mice, in drinking water, for 14 d. Regarding the non-inflammatory neonatal maternal separation (NMS) -induced CHS model, wild-type C57BI/6J pups were isolated from their mother from day 2 to day 14 (P2 to P14), three hours per day (from 9:00 a.m. to 12:00 p.m.). Colorectal distension was performed by inflating distension probe from 20 μL to 100 μL by 20 μL increment step every 10 s. After a first colorectal distension (CRD), drugs were administered subcutaneously, in a cumulative manner, (Gabapentin at 30 mg/kg and 100 mg/kg; Pregabalin at 10 mg/kg and 30 mg/kg; Carbamazepine at 10 mg/kg and 30 mg/kg) and a second CRD was performed one hour after each injection. RESULTS The visceromotor response (VMR) to CRD was increased by our NMS paradigm protocol in comparison to non-handled (NH) mice, considering the highest distension volumes (80 μL: 0.783 ± 0.056 mV/s vs 0.531 ± 0.034 mV/s, P NMS and NH mice, respectively). In the inflammation-associated CHS, DSS-treated mice showed a dramatic and significant increase in VMR at 60 and 80 μL distension volumes when compared to control mice (60 μL: 0.920 ± 0.079 mV/s vs 0.426 ± 0.100 mV/s P NMS-induced CHS model for the acute subcutaneous administration of the highest cumulative dose (30 mg/kg) and significantly reduced CHS in low-dose DSS-treated mice in a dose-dependent manner. Finally, the percent decrease of AUC induced by acute GBP or Pregabalin treatment were higher in the inflammatory DSS-induced CHS model in comparison to the non-inflammatory NMS-induced CHS model. CONCLUSION This preclinical study demonstrates α2δ-1 ligands efficacy on inflammation-associated CHS, highlighting their potential clinical interest in patients with chronic

  14. Polybrominated diphenyl ethers and their hydroxylated analogs in plasma of bottlenose dolphins (Tursiops truncatus) from the United States east coast.

    Science.gov (United States)

    Houde, Magali; Pacepavicius, Grazina; Darling, Colin; Fair, Patricia A; Alaee, Mehran; Bossart, Gregory D; Solomon, Keith R; Letcher, Robert J; Bergman, Ake; Marsh, Göran; Muir, Derek C G

    2009-10-01

    Polybrominated diphenyl ethers (PBDEs) and hydroxylated PBDEs (OH-PBDEs) were determined in plasma of free-ranging bottlenose dolphins (Tursiops truncatus) from Charleston (CHS), South Carolina, and the Indian River Lagoon (IRL), Florida, U.S.A. Significantly lower sums (sigma) of PBDE concentrations (sum of 12 congeners) were found in animals from the IRL (arithmetic mean, 5.45 +/- 4.63 ng/g wet wt) compared with those from CHS (30 +/- 40 ng/g wet wt). Brominated diphenyl ether (BDE)-47 was the predominant PBDE in dolphins from the IRL (50% of the sigma PBDEs) and CHS (58%). The sigma PBDE concentrations in plasma of dolphins were negatively correlated with age at both locations. Fifteen and sixteen individual OH-PBDE congeners could be quantified in plasma of dolphins from IRL and CHS, respectively. Similar to sigma PBDE, mean sigma OH-PBDE concentrations were significantly higher in plasma of dolphins at CHS (1150 +/- 708 pg/g wet wt) compared with those at IRL (624 +/- 393 pg/g wet wt). The predominant congener at both locations was 6-OH-PBDE-47 (IRL, 384 +/- 319 pg/g wet wt; CHS, 541 +/- 344 pg/g wet wt), representing 61.5% of total sigma OH-PBDE at IRL and 47.0% at CHS. Concentrations of sigma OH-PBDEs were weakly negatively correlated with age in dolphins from both locations (p < 0.05; IRL, r2 = 0.048; CHS, r2 = 0.021). In addition to the OH-PBDE congeners identified with technical standards, eight and four unidentified OH-PBDEs were detected and quantified, respectively, in animals from CHS (sum of unidentified OH-PBDEs = 1.35 +/- 0.90 pg/g wet wt) and IRL (0.73 +/- 0.40 pg/g wet wt). Results of the present study suggest that, unlike OH-PCBs, OH-PBDEs in bottlenose dolphins are minor products in plasma relative to sigma PBDEs and a significant proportion may be a consequence of the dietary uptake of naturally produced methoxylated- and OH-PBDEs.

  15. 南宁市城区社区卫生服务机构的SWOT分析%The SWOT Analyzing of Community Health Services in the District of Nanning City

    Institute of Scientific and Technical Information of China (English)

    郭伟; 倪建; 唐耀华; 梁贵琪; 张瑜

    2014-01-01

    On the basis of qualitative and quantitative research of Nanning city community health service institutions (CHS),in view of the situation of CHS development,using the SWOT analysis method to identify the internal strengths,weaknesses and external opportunities and threats of CHS institutions of Nanning city . Using SWOT matrix combination method to conduct system analysis, so as to put forward feasible suggestion and countermeasure for the existing problems in the process of CHS development , ultimately provide decision-making reference to promote the development of CHS Nanning city .%在对南宁市城区社区卫生服务(CHS)机构定性、定量调查的基础上,针对CHS发展的现状,利用SWOT分析法,识别南宁市城区CHS机构内部能力的优势、劣势和外部环境的机会及挑战。用SWOT的矩阵组合方法,对南宁市CHS发展过程中存在的问题进行系统分析,并提出可行的对策建议,为促进南宁市城区社区卫生服务事业的发展提供决策参考。

  16. Homozygosity mapping of the gene for Chediak-Higashi syndrome to chromosome 1q42-q44 in a segment of conserved synteny that includes the mouse beige locus (bg)

    Energy Technology Data Exchange (ETDEWEB)

    Fukai, Kazuyoshi; Oh, Jangsuk; Karim, M.A. [Univ. of Wisconsin Medical School, Madison, WI (United States)] [and others

    1996-09-01

    Chediak-Higashi syndrome (CHS) is an autosomal recessive disorder characterized by hypopigmentation or oculocutaneous albinism and severe immunologic deficiency with neutropenia and lack of natural killer (NK) cell function. Most patients die in childhood from pyogenic infections or an unusual lymphoma-like condition. A hallmark of the disorder is giant inclusion bodies seen in all granule-containing cells, including granulocytes, lymphocytes, melanocytes, mast cells, and neurons. Similar ultrastructural abnormalities occur in the beige mouse, which thus has been suggested to be homologous to human CHS. High-resolution genetic mapping has indicated that the bg gene region of mouse chromosome 13 is likely homologous to the distal portion of human chromosome 1q. Accordingly, we carried out homozygosity mapping using markers derived from distal human chromosome 1q in four inbred families or probands with CHS. Our results indicate that the human CHS gene maps to an 18.8-cM interval in chromosome segment 1q42-q44 and that human CHS therefore is very likely homologous to mouse bg. 43 refs., 2 figs.

  17. High resolution genetic mapping uncovers chitin synthase-1 as the target-site of the structurally diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole in Tetranychus urticae

    Science.gov (United States)

    Demaeght, Peter; Osborne, Edward J.; Odman-Naresh, Jothini; Grbić, Miodrag; Nauen, Ralf; Merzendorfer, Hans

    2014-01-01

    The acaricides clofentezine, hexythiazox and etoxazole are commonly referred to as ‘mite growth inhibitors’, and clofentezine and hexythiazox have been used successfully for the integrated control of plant mite pests for decades. Although they are still important today, their mode of action has remained elusive. Recently, a mutation in chitin synthase 1 (CHS1) was linked to etoxazole resistance. In this study, we identified and investigated a T. urticae strain (HexR) harboring recessive, monogenic resistance to each of hexythiazox, clofentezine, and etoxazole. To elucidate if there is a common genetic basis for the observed cross-resistance, we adapted a previously developed bulk segregant analysis method to map with high resolution a single, shared resistance locus for all three compounds. This finding indicates that the underlying molecular basis for resistance to all three compounds is identical. This locus is centered on the CHS1 gene, and as supported by additional genetic and biochemical studies, a non-synonymous variant (I1017F) in CHS1 associates with resistance to each of the tested acaricides in HexR. Our findings thus demonstrate a shared molecular mode of action for the chemically diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole as inhibitors of an essential, non-catalytic activity of CHS1. Given the previously documented cross-resistance between clofentezine, hexythiazox and the benzyolphenylurea compounds flufenoxuron and cycloxuron, CHS1 should be also considered as a potential target-site of insecticidal BPUs. PMID:24859419

  18. FUZZY LOGIC BASED ENERGY EFFICIENT PROTOCOL IN WIRELESS SENSOR NETWORKS

    Directory of Open Access Journals (Sweden)

    Zhan Wei Siew

    2012-12-01

    Full Text Available Wireless sensor networks (WSNs have been vastly developed due to the advances in microelectromechanical systems (MEMS using WSN to study and monitor the environments towards climates changes. In environmental monitoring, sensors are randomly deployed over the interest area to periodically sense the physical environments for a few months or even a year. Therefore, to prolong the network lifetime with limited battery capacity becomes a challenging issue. Low energy adaptive cluster hierarchical (LEACH is the common clustering protocol that aim to reduce the energy consumption by rotating the heavy workload cluster heads (CHs. The CHs election in LEACH is based on probability model which will lead to inefficient in energy consumption due to least desired CHs location in the network. In WSNs, the CHs location can directly influence the network energy consumption and further affect the network lifetime. In this paper, factors which will affect the network lifetime will be presented and the demonstration of fuzzy logic based CH selection conducted in base station (BS will also be carried out. To select suitable CHs that will prolong the network first node dies (FND round and consistent throughput to the BS, energy level and distance to the BS are selected as fuzzy inputs.

  19. Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.

    Directory of Open Access Journals (Sweden)

    Cheen Fei Chin

    2016-07-01

    Full Text Available Cytokinesis requires the spatio-temporal coordination of membrane deposition and primary septum (PS formation at the division site to drive acto-myosin ring (AMR constriction. It has been demonstrated that AMR constriction invariably occurs only after the mitotic spindle disassembly. It has also been established that Chitin Synthase II (Chs2p neck localization precedes mitotic spindle disassembly during mitotic exit. As AMR constriction depends upon PS formation, the question arises as to how chitin deposition is regulated so as to prevent premature AMR constriction and mitotic spindle breakage. In this study, we propose that cells regulate the coordination between spindle disassembly and AMR constriction via timely endocytosis of cytokinetic enzymes, Chs2p, Chs3p, and Fks1p. Inhibition of endocytosis leads to over accumulation of cytokinetic enzymes during mitotic exit, which accelerates the constriction of the AMR, and causes spindle breakage that eventually could contribute to monopolar spindle formation in the subsequent round of cell division. Intriguingly, the mitotic spindle breakage observed in endocytosis mutants can be rescued either by deleting or inhibiting the activities of, CHS2, CHS3 and FKS1, which are involved in septum formation. The findings from our study highlight the importance of timely endocytosis of cytokinetic enzymes at the division site in safeguarding mitotic spindle integrity during mitotic exit.

  20. Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica.

    Science.gov (United States)

    Lan, Xiaozhong; Quan, Hong; Xia, Xinli; Yin, Weilun; Zheng, Weilie

    2016-05-01

    Mirabilis himalaica is an endangered medicinal plant species in the Tibetan Plateau. The two genes respectively encoding chalcone synthase (MhCHS) and chalcone isomerase (MhCHI) were isolated and characterized from M. himalaica. The sequence analysis revealed that the two genes were similar with their corresponding homologous genes in other plants. The tissue profiles showed that both MhCHS and MhCHI had higher expression levels in roots than in stems and leaves. Transgenic hairy root cultures respectively with overexpressing MhCHS and MhCHI were established. The genomic PCR detection confirmed the authority of transgenic hairy root lines, in which either MhCHS or MhCHI expression levels were much higher than that in non-transgenic hairy root line. Finally, the HPLC detection results demonstrated that the rotenoid contents in MhCHS/MhCHI-transformed hairy root lines were enhanced. This study provided two candidate genes that could be used to genetic engineering rotenoid biosynthesis in M. himalaica and an alternative method to produce rotenoid using transgenic hairy root cultures.

  1. On triviality of S-matrix in conformal higher spin theory

    CERN Document Server

    Beccaria, M; Tseytlin, A A

    2016-01-01

    We consider the conformal higher spin (CHS) theory in d=4 that contains the s=1 Maxwell vector, s=2 Weyl graviton and their higher spin s=3,4,... counterparts with higher-derivative \\box^s kinetic terms. The interacting action for such theory can be found as the coefficient of the logarithmically divergent part in the induced action for sources coupled to higher spin currents in a free complex scalar field model. We explicitly determine some cubic and quartic interaction vertices in the CHS action from scalar loop integrals. We then compute the simplest tree-level 4-particle scattering amplitudes 11 -> 11, 22 -> 22 and 11 -> 22 and find that after summing up all the intermediate CHS exchanges they vanish. This generalises the vanishing of the scattering amplitude for external conformal scalars interacting via the exchange of all CHS fields found earlier in arXiv:1512.08896. This vanishing should generalise to all scattering amplitudes in the CHS theory and as in the conformal scalar scattering case should be ...

  2. Genetic and physical mapping of the Chediak-Higashi syndrome on chromosome 1q42-43

    Energy Technology Data Exchange (ETDEWEB)

    Barrat, F.J.; Auloge, L.; Pastural, E. [INSERM, Paris (France)] [and others

    1996-09-01

    The Chediak-Higashi syndrome (CHS) is a severe autosomal recessive condition, features of which are partial oculocutaneous albinism, increased susceptibility to infections, deficient natural killer cell activity, and the presence of large intracytoplasmic granulations in various cell types. Similar genetic disorders have been described in other species, including the beige mouse. On the basis of the hypothesis that the murine chromosome 13 region containing the beige locus was homologous to human chromosome 1, we have mapped the CHS locus to a 5-cM interval in chromosome segment 1q42.1-q42.2. The highest LOD score was obtained with the marker D1S235 (Z{sub max} = 5.38; {theta} = 0). Haplotype analysis enabled us to establish D1S2680 and D1S163, respectively, as the telomeric and the centromeric flanking markers. Multipoint linkage analysis confirms the localization of the CHS locus in this interval. Three YAC clones were found to cover the entire region in a contig established by YAC end-sequence characterization and sequence-tagged site mapping. The YAC contig contains all genetic markers that are nonrecombinant for the disease in the nine CHS families studied. This mapping confirms the previous hypothesis that the same gene defect causes CHS in human and beige phenotype in mice and provides a genetic framework for the identification of candidate genes. 36 refs., 4 figs., 1 tab.

  3. CHSalign: A Web Server That Builds upon Junction-Explorer and RNAJAG for Pairwise Alignment of RNA Secondary Structures with Coaxial Helical Stacking.

    Directory of Open Access Journals (Sweden)

    Lei Hua

    Full Text Available RNA junctions are important structural elements of RNA molecules. They are formed when three or more helices come together in three-dimensional space. Recent studies have focused on the annotation and prediction of coaxial helical stacking (CHS motifs within junctions. Here we exploit such predictions to develop an efficient alignment tool to handle RNA secondary structures with CHS motifs. Specifically, we build upon our Junction-Explorer software for predicting coaxial stacking and RNAJAG for modelling junction topologies as tree graphs to incorporate constrained tree matching and dynamic programming algorithms into a new method, called CHSalign, for aligning the secondary structures of RNA molecules containing CHS motifs. Thus, CHSalign is intended to be an efficient alignment tool for RNAs containing similar junctions. Experimental results based on thousands of alignments demonstrate that CHSalign can align two RNA secondary structures containing CHS motifs more accurately than other RNA secondary structure alignment tools. CHSalign yields a high score when aligning two RNA secondary structures with similar CHS motifs or helical arrangement patterns, and a low score otherwise. This new method has been implemented in a web server, and the program is also made freely available, at http://bioinformatics.njit.edu/CHSalign/.

  4. An unusual very low-mass high-amplitude pre-main sequence periodic variable

    CERN Document Server

    Rodriguez-Ledesma, Maria V; Ibrahimov, Mansur; Messina, Sergio; Parihar, Padmakar; Hessman, Frederic; de Oliveira, Catarina Alves; Herbst, William

    2012-01-01

    We have investigated the nature of the variability of CHS7797, an unusual periodic variable in the Orion Nebula Cluster. An extensive I-band photometric data set of CHS7797 was compiled between 2004-2010 using various telescopes. Further optical data have been collected in R and z' bands. In addition, simultaneous observations of the ONC region including CHS7797 were performed in the I, J, Ks and IRAC [3.6] and [4.5] bands over a time interval of about 40d. CHS7797 shows an unusual large-amplitude variation of about 1.7 mag in the R, I, and z' bands with a period 17.786. The amplitude of the brightness modulation decreases only slightly at longer wavelengths. The star is faint during 2/3 of the period and the shape of the phased light-curves for seven different observing seasons shows minor changes and small-amplitude variations. Interestingly, there are no significant colour-flux correlations for wavelengths smaller than 2microns, while the object becomes redder when fainter at longer wavelengths. CHS7797 ha...

  5. Differential modification of flavonoid and isoflavonoid biosynthesis with an antisense chalcone synthase construct in transgenic Lotus corniculatus.

    Science.gov (United States)

    Colliver, S P; Morris, P; Robbins, M P

    1997-11-01

    Three clonal genotypes of Lotus corniculatus L. (bird's foot trefoil) were transformed with an antisense chalcone synthase (CHS) gene construct made using a stress induced CHS17 cDNA from Phaseolus vulgaris under the control of the constitutive CaMV 35S promoter and Nos terminator via Agrobacterium rhizogenes. After initial screening, ten antisense and five control co-transformation events from each recipient clonal genotype were analysed. After elicitation with glutathione, the level of tannin accumulation was found to be increased in a number of antisense root cultures derived from the low (S33) and moderate (S50) tannin recipient genotypes. Six antisense and four control transformed lines from genotype S50 were selected for more detailed study. The antisense CHS construct was found to be integrated into the genome, with a copy number ranging from 1 to 5 and antisense orientation was confirmed by PCR. In transformed root cultures, increased CHS transcript levels were noted in a number of antisense lines. Biochemical analyses of glutathione-elicited-root cultures indicated a significant increase in tannin accumulation in antisense CHS lines and mean vestitol levels were reduced. These results show that the introduction of a heterologous antisense chalcone synthase construct into L. corniculatus resulted in an unpredicted molecular and biochemical phenotype. Such findings are discussed in relation to manipulation of this complex multigene family.

  6. Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites.

    Science.gov (United States)

    Zhao, Ya-e; Wang, Zheng-hang; Xu, Yang; Xu, Ji-ru; Liu, Wen-yan; Wei, Meng; Wang, Chu-ying

    2012-10-01

    To our knowledge, few reports on Demodex studied at the molecular level are available at present. In this study our group, for the first time, cloned, sequenced and analyzed the chitin synthase (CHS) gene fragments of Demodex folliculorum, Demodex brevis, and Demodex canis (three isolates from each species) from Xi'an China, by designing specific primers based on the only partial sequence of the CHS gene of D. canis from Japan, retrieved from GenBank. Results show that amplification was successful only in three D. canis isolates and one D. brevis isolate out of the nine Demodex isolates. The obtained fragments were sequenced to be 339 bp for D. canis and 338 bp for D. brevis. The CHS gene sequence similarities between the three Xi'an D. canis isolates and one Japanese D. canis isolate ranged from 99.7% to 100.0%, and those between four D. canis isolates and one D. brevis isolate were 99.1%-99.4%. Phylogenetic trees based on maximum parsimony (MP) and maximum likelihood (ML) methods shared the same clusters, according with the traditional classification. Two open reading frames (ORFs) were identified in each CHS gene sequenced, and their corresponding amino acid sequences were located at the catalytic domain. The relatively conserved sequences could be deduced to be a CHS class A gene, which is associated with chitin synthesis in the integument of Demodex mites.

  7. Imaging Evidence for Cerebral Hyperperfusion Syndrome after Intravenous Tissue Plasminogen Activator for Acute Ischemic Stroke

    Directory of Open Access Journals (Sweden)

    Yi Zhang

    2016-01-01

    Full Text Available Background. Cerebral hyperperfusion syndrome (CHS, a rare complication after cerebral revascularization, is a well-described phenomenon after carotid endarterectomy or carotid artery stenting. However, the imaging evidence of CHS after intravenous tissue plasminogen activator (iv tPA for acute ischemic stroke (AIS has not been reported. Case Report. Four patients were determined to have manifestations of CHS with clinical deterioration after treatment with iv tPA, including one patient who developed seizure, one patient who had a deviation of the eyes toward lesion with worsened mental status, and two patients who developed worsened hemiparesis. In all four patients, postthrombolysis head CT examinations were negative for hemorrhage; CT angiogram showed patent cervical and intracranial arterial vasculature; CT perfusion imaging revealed hyperperfusion with increased relative cerebral blood flow and relative cerebral blood volume and decreased mean transit time along with decreased time to peak in the clinically related artery territory. Vascular dilation was also noted in three of these four cases. Conclusions. CHS should be considered in patients with clinical deterioration after iv tPA and imaging negative for hemorrhage. Cerebral angiogram and perfusion studies can be useful in diagnosing CHS thereby helping with further management.

  8. High resolution genetic mapping uncovers chitin synthase-1 as the target-site of the structurally diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole in Tetranychus urticae.

    Science.gov (United States)

    Demaeght, Peter; Osborne, Edward J; Odman-Naresh, Jothini; Grbić, Miodrag; Nauen, Ralf; Merzendorfer, Hans; Clark, Richard M; Van Leeuwen, Thomas

    2014-08-01

    The acaricides clofentezine, hexythiazox and etoxazole are commonly referred to as 'mite growth inhibitors', and clofentezine and hexythiazox have been used successfully for the integrated control of plant mite pests for decades. Although they are still important today, their mode of action has remained elusive. Recently, a mutation in chitin synthase 1 (CHS1) was linked to etoxazole resistance. In this study, we identified and investigated a Tetranychus urticae strain (HexR) harboring recessive, monogenic resistance to each of hexythiazox, clofentezine, and etoxazole. To elucidate if there is a common genetic basis for the observed cross-resistance, we adapted a previously developed bulk segregant analysis method to map with high resolution a single, shared resistance locus for all three compounds. This finding indicates that the underlying molecular basis for resistance to all three compounds is identical. This locus is centered on the CHS1 gene, and as supported by additional genetic and biochemical studies, a non-synonymous variant (I1017F) in CHS1 associates with resistance to each of the tested acaricides in HexR. Our findings thus demonstrate a shared molecular mode of action for the chemically diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole as inhibitors of an essential, non-catalytic activity of CHS1. Given the previously documented cross-resistance between clofentezine, hexythiazox and the benzyolphenylurea (BPU) compounds flufenoxuron and cycloxuron, CHS1 should be also considered as a potential target-site of insecticidal BPUs.

  9. Metabolic engineering of the morphology of Aspergillus oryzae by altering chitin synthesis

    DEFF Research Database (Denmark)

    Muller, C.; Mcintyre, Mhairi; Hansen, Kim;

    2002-01-01

    Morphology and alpha-amylase production during submerged cultivation were examined in a wild-type strain (A1560) and in strains of Aspergillus oryzae in which chitin synthase B (chsB) and chitin synthesis myosin A (csmA) have been disrupted (ChsB/G and CM101). In a flowthrough cell, the growth...... than that in the wild type., whereas that in the ChsB/G strain was 188% higher. During batch cultivation, inseparable clumps were formed in the wild-type strain., while no or fewer large inseparable clumps existed in the cultivations of the ChsB/G and CM101 strains. The alpha-amylase productivity...... an important role for chsB in branching. However, the pattern of branching responded very slowly to the change in transcription, and increased branching did not affect alpha-amylase productivity. alpha-Amylase residing in the cell wall was stained by immunofluorescence, and the relationship between tip number...

  10. Modification of a viral satellite DNA-based gene silencing vector and its application to leaf or flower color change in Petunia hybrida

    Institute of Scientific and Technical Information of China (English)

    TAO Xiaorong; QIAN Yajuan; ZHOU Xueping

    2006-01-01

    Virus-induced gene silencing offers a powerful reverse-genetic tool for the study of gene function in plants. We have previously reported effective gene silencing of plant genes using a viral satellite DNA associated with Tomato yellow leaf curl China virus (TYLCCNV). In this study, we further modified the viral satellite DNA-based vector. The modified vector can induce sulfu (Su) gene silencing as effective as the original vector in Nicotiana benthamiana plants, but the new system simplifies procedures for construction of vector derivative. Furthermore, a fragment of petunia Su or chalcone synthase (CHS) endogenous gene was inserted into the modified vector. When petunia plants were agro- inoculated with the modified vector carrying a Su or CHS gene, the Su silenced plants started to appear yellowing in veins of systemically infected upper leaves two weeks after agroinoculation, while the CHS silenced plants started to show flower color change one month after agroinoculation and later single-color flowers became mosaic.

  11. Benzophenone Synthase and Chalcone Synthase Accumulate in the Mesophyll of Hypericum perforatum Leaves at Different Developmental Stages

    Science.gov (United States)

    Belkheir, Asma K.; Gaid, Mariam; Liu, Benye; Hänsch, Robert; Beerhues, Ludger

    2016-01-01

    The active medicinal constituents in Hypericum perforatum, used to treat depression and skin irritation, include flavonoids and xanthones. The carbon skeletons of these compounds are formed by chalcone synthase (CHS) and benzophenone synthase (BPS), respectively. Polyclonal antisera were raised against the polyketide synthases from Hypericum androsaemum and their IgG fractions were isolated. Immunoblotting and immunotitration were used to test the IgGs for crossreactivity and monospecificity in H. perforatum leaf protein extract. Immunofluorescence localization revealed that both CHS and BPS are located in the mesophyll. The maximum fluorescence levels were observed in approx. 0.5 and 1 cm long leaves, respectively. The fluorescence intensity observed for CHS significantly exceeded that for BPS. Using histochemical staining, flavonoids were detected in the mesophyll, indicating that the sites of biosynthesis and accumulation coincide. Our results help understand the biosynthesis and underlying regulation of active H. perforatum constituents. PMID:27446151

  12. Conformal higher spin scattering amplitudes from twistor space

    CERN Document Server

    Adamo, Tim; McLoughlin, Tristan

    2016-01-01

    We use the formulation of conformal higher spin (CHS) theories in twistor space to study their tree-level scattering amplitudes, finding expressions for all three-point anti-MHV amplitudes and all MHV amplitudes involving positive helicity conformal gravity particles and two negative helicity higher spins. This provides the on-shell analogue for the covariant coupling of CHS fields to a conformal gravity background. We discuss the restriction of the theory to a ghost-free unitary subsector, analogous to restricting conformal gravity to general relativity with a cosmological constant. We study the flat-space limit and show that the restricted amplitudes vanish, supporting the conjecture that in the unitary sector the S-matrix of CHS theories is trivial. However, by appropriately rescaling the amplitudes we find non-vanishing results which we compare with chiral flat-space higher spin theories.

  13. Benzophenone Synthase and Chalcone Synthase Accumulate in the Mesophyll of Hypericum perforatum Leaves at Different Developmental Stages.

    Science.gov (United States)

    Belkheir, Asma K; Gaid, Mariam; Liu, Benye; Hänsch, Robert; Beerhues, Ludger

    2016-01-01

    The active medicinal constituents in Hypericum perforatum, used to treat depression and skin irritation, include flavonoids and xanthones. The carbon skeletons of these compounds are formed by chalcone synthase (CHS) and benzophenone synthase (BPS), respectively. Polyclonal antisera were raised against the polyketide synthases from Hypericum androsaemum and their IgG fractions were isolated. Immunoblotting and immunotitration were used to test the IgGs for crossreactivity and monospecificity in H. perforatum leaf protein extract. Immunofluorescence localization revealed that both CHS and BPS are located in the mesophyll. The maximum fluorescence levels were observed in approx. 0.5 and 1 cm long leaves, respectively. The fluorescence intensity observed for CHS significantly exceeded that for BPS. Using histochemical staining, flavonoids were detected in the mesophyll, indicating that the sites of biosynthesis and accumulation coincide. Our results help understand the biosynthesis and underlying regulation of active H. perforatum constituents.

  14. Effect of Energetic-Ion-Driven MHD Instabilities on Energetic-Ion-Transport in Compact Helical System and Large Helical Device

    Energy Technology Data Exchange (ETDEWEB)

    Isobe, M. [National Institute for Fusion Science, Toki, Japan; Ogawa, K. [Nagoya University, Japan; Toi, K. [National Institute for Fusion Science, Toki, Japan; Osakabe, M. [National Institute for Fusion Science, Toki, Japan; Nagaoka, K. [National Institute for Fusion Science, Toki, Japan; Shimizu, A. [National Institute for Fusion Science, Toki, Japan; Spong, Donald A [ORNL; Okumura, S. [National Institute for Fusion Science, Toki, Japan

    2010-01-01

    This paper describes 1) representative results on excitation of energetic-particle mode (EPM) and toroidicity-induced Alfven eigenmode (TAE) and consequent beam-ion losses in CHS, and 2) recent results on beam-ion transport and/or losses while EPMs are destabilized in LHD. Bursting EPMs and TAEs are often excited by co-injected beam ions in the high-beam ion pressure environment and give a significant effect on co-going beam ions in both experiments. It seems that in CHS, resonant beam ions are lost within a relatively short-time scale once they are anomalously transported due to energetic-ion driven MHD modes, whereas unlike CHS, redistribution of beam ions due to energetic-ion driven MHD modes is seen in LHD, suggesting that not all anomalously transported beam ions escape from the plasma.

  15. Effect of energetic-ion-driven MHD instabilities on energetic-ion-transport in compact helical system and large helical device

    Energy Technology Data Exchange (ETDEWEB)

    Isobe, M.; Toi, K.; Osakabe, M.; Nagaoka, K.; Shimizu, A.; Okamura, S. [National Institute for Fusion Science, Toki (Japan); Ogawa, K. [Department of Energy Science and Engineering, Nagoya University, Nagoya (Japan); Spong, D.A. [Oak Ridge National Laboratory, Oak Ridge, TN (United States)

    2010-08-15

    This paper describes (1) representative results on excitation of energetic-particle mode (EPM) and toroidicity-induced Alfven eigenmode (TAE) and consequent beam-ion losses in CHS, and (2) recent results on beam-ion transport and/or losses while EPMs are destabilized in LHD. Bursting EPMs and TAEs are often excited by co-injected beam ions in the high-beam ion pressure environment and give a significant effect on co-going beam ions in both experiments. It seems that in CHS, resonant beam ions are lost within a relatively short-time scale once they are anomalously transported due to energetic-ion driven MHD modes, whereas unlike CHS, redistribution of beam ions due to energetic-ion driven MHD modes is seen in LHD, suggesting that not all anomalously transported beam ions escape from the plasma. (copyright 2010 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  16. 非洲菊查尔酮合酶基因的克隆、序列分析及在大肠杆菌中的表达%Chalcone Synthase Gene Cloning in Gerbera hybrida and Expression in E.coli

    Institute of Scientific and Technical Information of China (English)

    谢修志; 陈兆平; 王小菁

    2004-01-01

    利用RT-PCR方法,从非洲菊(Gerbera hybrida)花瓣的cDNA中克隆到了查尔酮合酶(Chalcone Synthase,CHS)基因CHS,进行了序列分析.结果表明,克隆到的CHS基因全长为1197 bps,编码一个由398个氨基酸残基组成的多肽,与Helariutta等发表的非洲菊查尔酮合酶CHS1基因的cDNA序列的CHS基因同源性高达99%.进一步将该基因克隆到表达载体pET32a上,经IPTG诱导表达,得到高效表达的融合蛋白.

  17. Lowest-ID with Adaptive ID Reassignment: A Novel Mobile Ad-Hoc Networks Clustering Algorithm

    CERN Document Server

    Gavalas, Damianos; Konstantopoulos, Charalampos; Mamalis, Basilis

    2011-01-01

    Clustering is a promising approach for building hierarchies and simplifying the routing process in mobile ad-hoc network environments. The main objective of clustering is to identify suitable node representatives, i.e. cluster heads (CHs), to store routing and topology information and maximize clusters stability. Traditional clustering algorithms suggest CH election exclusively based on node IDs or location information and involve frequent broadcasting of control packets, even when network topology remains unchanged. More recent works take into account additional metrics (such as energy and mobility) and optimize initial clustering. However, in many situations (e.g. in relatively static topologies) re-clustering procedure is hardly ever invoked; hence initially elected CHs soon reach battery exhaustion. Herein, we introduce an efficient distributed clustering algorithm that uses both mobility and energy metrics to provide stable cluster formations. CHs are initially elected based on the time and cost-efficien...

  18. Chédiak-Higashi syndrome: presentation of seven cases

    Directory of Open Access Journals (Sweden)

    Eugénia Maria Grilo Carnide

    Full Text Available CONTEXT: Chédiak-Higashi Syndrome (CHS is a rare autosomal recessive disease characterized by recurrent infections, giant cytoplasmic granules, and oculocutaneous albinism. OBJECTIVE: To describe clinical and laboratory findings from CHS patients. DESIGN: Case report. SETTING: The patients were admitted into the Allergy and Immunology Unit of the Instituto da Criança, a tertiary public care institution. CASES REPORT: Seven patients had oculocutaneous albinism, recurrent infections and giant cytoplasmic granules in the leukocytes. One patient had low IgG levels and three showed impaired bactericidal activity of neutrophils. Six patients died of infectious complications during the accelerated phase. Therapy included ascorbic acid and antibiotics. Chemotherapy was used for the accelerated phase in two patients. Bone marrow transplantation (BMT was proposed for one patient. DISCUSSION: The authors emphasize the need for early diagnosis and therapy of CHS. BMT should be indicated before the accelerated phase of the disease has developed.

  19. New Role of Rosea1 in Regulating Anthocyanin Biosynthetic Pathway in Hairy Root of Snapdragon (Antirrhinum majus L.

    Directory of Open Access Journals (Sweden)

    An Zhang

    2013-09-01

    Full Text Available We investigated the transcriptional regulation of anthocyanin biosynthesis in hairy roots system by ectopically expressing Rosea1 and Delila and we found something different from previous research. The RT-PCR results revealed that Rosea1 could activate early and late biosynthetic genes tested, including CHS, DFR and ANS. Delila enhanced the expression of CHS weakly, but did not influence DFR or ANS. The two regulators, Rosea1 and Delila, failed to interplay each other. It was speculated that Delila would be ineffective in the absence of Rosea1, another MYB factor specifically controlling CHS may exist. This investigation provided a new way to increase anthocyanin content by over expressing a MYB factor, potentially to be used in the field of agriculture and food

  20. The Memory Alteration Test Discriminates between Cognitively Healthy Status, Mild Cognitive Impairment and Alzheimer's Disease

    Science.gov (United States)

    Custodio, Nilton; Lira, David; Herrera-Perez, Eder; Nuñez del Prado, Liza; Parodi, José; Guevara-Silva, Erik; Castro-Suarez, Sheila; Montesinos, Rosa; Cortijo, Patricia

    2014-01-01

    Background/Aims Dementia is a worldwide public health problem and there are several diagnostic tools for its assessment. The aim of this study was to evaluate the performance of the Memory Alteration Test (M@T) to discriminate between patients with early Alzheimer's disease (AD), patients with amnestic mild cognitive impairment (a-MCI), and subjects with a cognitively healthy status (CHS). Methods The discriminative validity was assessed in a sample of 90 patients with AD, 45 patients with a-MCI, and 180 subjects with CHS. Clinical, functional, and cognitive studies were independently performed in a blinded fashion and the gold standard diagnosis was established by consensus on the basis of these results. The test performance was assessed by means of a receiver operating characteristic curve analysis as area under the curve (AUC). Results M@T mean scores were 17.7 (SD = 5.7) in AD, 30.8 (SD = 2.3) in a-MCI, and 44.5 (SD = 3.1) in CHS. A cutoff score of 37 points had a sensitivity of 98.3% and a specificity of 97.8% to differentiate a-MCI from CHS (AUC = 0.999). A cutoff score of 27 points had a sensitivity of 100% and a specificity of 98.9% to differentiate mild AD from a-MCI and from CHS (AUC = 1.000). Conclusions The M@T had a high performance in the discrimination between early AD, a-MCI and CHS. PMID:25298775

  1. The brain functional magnetic resonance imaging characteristics induced by contact heat stimulations in normal adults%接触性热痛刺激健康成年人诱发的脑功能磁共振成像初步研究

    Institute of Scientific and Technical Information of China (English)

    谭娟; 王晓明; 罗成; 涂世鹏; 崔文娟; 张耀丹

    2014-01-01

    Objective Aδ and C fiber nociceptors can be excited when people received contact heat stimulation(CHS) and this excitation may be reflected by functional magnetic resonance imaging(fMRI).The aim of this study was to observe the different fMRI characteristics in CHS with distinct temperatures in healthy people and to explore the pain related functional network.Depending on the fMRI results,we can evaluate the values of CHS-fMRI during the research of brain functional connectivity.Methods Twenty-two healthy volunteers were recruited to this study.During the acquisition of fMRI,the right dorsal forearm received two different CHS in 41 and 51 degree respectively(41 ℃ CHS-fMRI group and 51 ℃ CHS-fMRI group),and two series of fMRI data were obtained for each subject.The brain activation was obtained by using one sample t test for the 41 ℃CHS-fMRI group and 51 ℃CHS-fMRI group separately.The difference between 41 ℃ CHS-fMRI group and 51 ℃ CHS-fMRI group was analyzed by paired-sample t-test.Result These activated brain areas in 41 ℃ CHS-fMRI group included bilateral superior temporal gyrus (STG),contralateral transverse temporal gyrus,contralateral amygdala,bilateral insula,bilateral inferior frontal gyrus (IFG),contralateral hippocampus,bilateral putamen,contralateral supramarginal,ipsilateral postcentral,ipsilateral inferior parietal lobule,ipsilateral middle temporal gyrus (MTG),bilateral cerebelum,bilateral middle cingulate cortex and bilateral posterior cingulate cortex.These activated brain areas in 51 ℃ CHS-fMRI group included bilateral STG,bilateral amygdala,contralateral hippocampus,bilateral thalamus,contralateral putamen,bilateral insula,bilateral IFG,bilateral cerebellum,contralateral postcentral,contralateral superior parietal lobule,bilateral MTG,bilateral precuneus,and contralateral cuneus.Compared with 41 ℃ CHS-fMRI group,the 51 ℃ CHS-fMRI group showed higher brain activation in bilateral cerebellum (right:4.455,left:3.891),ipsilateral

  2. Non-invasive assessment of cerebral microcirculation with diffuse optics and coherent hemodynamics spectroscopy

    Science.gov (United States)

    Fantini, Sergio; Sassaroli, Angelo; Kainerstorfer, Jana M.; Tgavalekos, Kristen T.; Zang, Xuan

    2016-03-01

    We describe the general principles and initial results of coherent hemodynamics spectroscopy (CHS), which is a new technique for the quantitative assessment of cerebral hemodynamics on the basis of dynamic near-infrared spectroscopy (NIRS) measurements. The two components of CHS are (1) dynamic measurements of coherent cerebral hemodynamics in the form of oscillations at multiple frequencies (frequency domain) or temporal transients (time domain), and (2) their quantitative analysis with a dynamic mathematical model that relates the concentration and oxygen saturation of hemoglobin in tissue to cerebral blood volume (CBV), cerebral blood flow (CBF), and cerebral metabolic rate of oxygen (CMRO2). In particular, CHS can provide absolute measurements and dynamic monitoring of CBF, and quantitative measures of cerebral autoregulation. We report initial results of CBF measurements in hemodialysis patients, where we found a lower CBF (54 +/- 16 ml/(100 g-min)) compared to a group of healthy controls (95 +/- 11 ml/(100 g-min)). We also report CHS measurements of cerebral autoregulation, where a quantitative index of autoregulation (its cutoff frequency) was found to be significantly greater in healthy subjects during hyperventilation (0.034 +/- 0.005 Hz) than during normal breathing (0.017 +/- 0.002 Hz). We also present our approach to depth resolved CHS, based on multi-distance, frequency-domain NIRS data and a two-layer diffusion model, to enhance sensitivity to cerebral tissue. CHS offers a potentially powerful approach to the quantitative assessment and continuous monitoring of local brain perfusion at the microcirculation level, with prospective brain mapping capabilities of research and clinical significance.

  3. Morphological changes induced by class III chitin synthase gene silencing could enhance penicillin production of Penicillium chrysogenum.

    Science.gov (United States)

    Liu, Hui; Zheng, Zhiming; Wang, Peng; Gong, Guohong; Wang, Li; Zhao, Genhai

    2013-04-01

    Chitin synthases catalyze the formation of β-(1,4)-glycosidic bonds between N-acetylglucosamine residues to form the unbranched polysaccharide chitin, which is the major component of cell walls in most filamentous fungi. Several studies have shown that chitin synthases are structurally and functionally divergent and play crucial roles in the growth and morphogenesis of the genus Aspergillus although little research on this topic has been done in Penicillium chrysogenum. We used BLAST to find the genes encoding chitin synthases in P. chrysogenum related to chitin synthase genes in Aspergillus nidulans. Three homologous sequences coding for a class III chitin synthase CHS4 and two hypothetical proteins in P. chrysogenum were found. The gene which product showed the highest identity and encoded the class III chitin synthase CHS4 was studied in detail. To investigate the role of CHS4 in P. chrysogenum morphogenesis, we developed an RNA interference system to silence the class III chitin synthase gene chs4. After transformation, mutants exhibited a slow growth rate and shorter and more branched hyphae, which were distinct from those of the original strain. The results also showed that the conidiation efficiency of all transformants was reduced sharply and indicated that chs4 is essential in conidia development. The morphologies of all transformants and the original strain in penicillin production were investigated by light microscopy, which showed that changes in chs4 expression led to a completely different morphology during fermentation and eventually caused distinct penicillin yields, especially in the transformants PcRNAi1-17 and PcRNAi2-1 where penicillin production rose by 27 % and 41 %, respectively.

  4. CD1d knockout mice exhibit aggravated contact hypersensitivity responses due to reduced interleukin-10 production predominantly by regulatory B cells

    DEFF Research Database (Denmark)

    Fjelbye, Jonas; Antvorskov, Julie C; Buschard, Karsten

    2015-01-01

    Conflicting observations have been reported concerning the role of CD1d-dependent natural killer T (NKT) cells in contact hypersensitivity (CHS), supporting either a disease-promoting or downregulatory function. We studied the role of NKT cells in CHS by comparing the immune response in CD1d.......5% DNCB (w/v) on the ears fifteen days later. We demonstrate that CD1d KO mice, as compared with Wt littermates, have more pronounced infiltration of mononuclear cells in the skin (29.1% increase; P

  5. Cholesterol increases kinetic, energetic, and mechanical stability of the human β2-adrenergic receptor

    DEFF Research Database (Denmark)

    Zocher, Michael; Zhang, Cheng; Rasmussen, Søren Gøgsig Faarup;

    2012-01-01

    the kinetic, energetic, and mechanical stability of almost every structural segment at sufficient magnitude to alter the structure and functional relationship of β(2)AR. One exception was the structural core segment of β(2)AR, which establishes multiple ligand binding sites, and its properties were...... to quantify the mechanical strength and flexibility, conformational variability, and kinetic and energetic stability of structural segments stabilizing the human β(2)-adrenergic receptor (β(2)AR) in the absence and presence of the cholesterol analog cholesteryl hemisuccinate (CHS). CHS considerably increased...

  6. Main: CPRFPCCHS [PLACE

    Lifescience Database Archive (English)

    Full Text Available CPRFPCCHS S000313 8-February-2006 (last modified) kehi BoxII; Binding site of CPRF-...1, -2, -3 and -4(Common Plant Regulatory Factor) in the parsley (P.c.) light responsive chalcone synthase (CHS) gene promoter; CPRF... proteins are bZIP class transcription factors; CPRF proteins participates in the lig...ht-mediated activation of the CHS gene in parsley; ACE; The proline-rich domains of CPRF...1 and 4 activate transcription; CPRF1-containing bZIP heterodimer interacts with ACE in vivo; BoxII; CPRF

  7. Building partnerships towards strengthening Makerere University College of Health Sciences: a stakeholder and sustainability analysis

    Directory of Open Access Journals (Sweden)

    Pariyo George W

    2011-03-01

    Full Text Available Abstract Background Partnerships and networking are important for an institution of higher learning like Makerere University College of Health Sciences (MakCHS to be competitive and sustainable. Methods A stakeholder and sustainability analysis of 25 key informant interviews was conducted among past, current and potential stakeholders of MakCHS to obtain their perspectives and contributions to sustainability of the College in its role to improve health outcomes. Results The College has multiple internal and external stakeholders. Stakeholders from Uganda wanted the College to use its enormous academic capacity to fulfil its vision, take initiative, and be innovative in conducting more research and training relevant to the country’s health needs. Many stakeholders felt that the initiative for collaboration currently came more from the stakeholders than the College. External stakeholders felt that MakCHS was insufficiently marketing itself and not directly engaging the private sector or Parliament. Stakeholders also identified the opportunity for MakCHS to embrace information technology in research, learning and training, and many also wanted MakCHS to start leadership and management training programmes in health systems. The need for MakCHS to be more vigorous in training to enhance professionalism and ethical conduct was also identified. Discussion As a constituent of a public university, MakCHS has relied on public funding, which has been inadequate to fulfill its mission. Broader networking, marketing to mobilize resources, and providing strong leadership and management support to inspire confidence among its current and potential stakeholders will be essential to MakCHS’ further growth. MakCHS’ relevance is hinged on generating research knowledge for solving the country’s contemporary health problems and starting relevant programs and embracing technologies. It should share new knowledge widely through publications and other forms of

  8. Makerere University College of Health Sciences’ role in addressing challenges in health service provision at Mulago National Referral Hospital

    Directory of Open Access Journals (Sweden)

    Sekandi Juliet

    2011-03-01

    Full Text Available Abstract Background Mulago National Referral Hospital (MNRH, Uganda’s primary tertiary and teaching hospital, and Makerere University College of Health Sciences (MakCHS have a close collaborative relationship. MakCHS students complete clinical rotations at MNRH, and MakCHS faculty partner with Mulago staff in clinical care and research. In 2009, as part of a strategic planning process, MakCHS undertook a qualitative study to examine care and service provision at MNRH, identify challenges, gaps, and solutions, and explore how MakCHS could contribute to improving care and service delivery at MNRH. Methods Key informant interviews (n=23 and focus group discussions (n=7 were conducted with nurses, doctors, administrators, clinical officers and other key stakeholders. Interviews and focus groups were tape recorded and transcribed verbatim, and findings were analyzed through collaborative thematic analysis. Results Challenges to care and service delivery at MNRH included resource constraints (staff, space, equipment, and supplies, staff inadequacies (knowledge, motivation, and professionalism, overcrowding, a poorly functioning referral system, limited quality assurance, and a cumbersome procurement system. There were also insufficiencies in the teaching of professionalism and communication skills to students, and patient care challenges that included lack of access to specialized services, risk of infections, and inappropriate medications. Suggestions for how MakCHS could contribute to addressing these challenges included strengthening referral systems and peripheral health center capacity, and establishing quality assurance mechanisms. The College could also strengthen the teaching of professionalism, communication and leadership skills to students, and monitor student training and develop courses that contribute to continuous professional development. Additionally, the College could provide in-service education for providers on professionalism

  9. AcEST: BP913294 [AcEST

    Lifescience Database Archive (English)

    Full Text Available lease 56.9) Link to BlastX Result : Swiss-Prot sp_hit_id P23569 Definition sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria...Score E Sequences producing significant alignments: (bits) Value sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria...10 sp|Q9ZS40|CHS2_DAUCA Chalcone synthase 2 OS=Daucus carota GN=CHS... 64 3e-10 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria

  10. Trace element concentrations in skin of free-ranging bottlenose dolphins (Tursiops truncatus) from the southeast Atlantic coast.

    Science.gov (United States)

    Stavros, Hui-Chen W; Bossart, Gregory D; Hulsey, Thomas C; Fair, Patricia A

    2007-12-15

    Concentrations of trace elements (Al, As, Ba, Be, Cd, Co, Cr, Cu, Fe, Li, Mn, Ni, Pb, Sb, Se, Sn, Tl, U, V, Zn) and total mercury (THg) were determined in skin samples collected from free-ranging bottlenose dolphin (Tursiops truncatus) populations. Dolphins were captured in the estuarine waters of Charleston (CHS), South Carolina (n=74) and the Indian River Lagoon (IRL), Florida (n=75) during 2003, 2004 and 2005. A subset of the skin tissue samples were used to determine methylmercury (MeHg) levels in CHS (n=17) and IRL (n=8) bottlenose dolphins. Distributions of trace element concentrations by age (adult vs. juvenile), gender (male vs. female) and study area (CHS vs. IRL) were examined. In general, higher elemental skin concentrations were found in CHS adult males than those of IRL adult males, except for THg and MeHg. For CHS dolphins, adult females showed significantly higher THg levels than juvenile females while higher Mn levels were found in juvenile females. For IRL dolphins, adult males showed significantly higher As concentrations than that in juvenile males and females while higher Co and V levels were found in juvenile males than adult males. Of all elements measured in this study, significantly higher levels of Fe, Se and Zn concentrations in skin tissue of both dolphin populations were similar to other studies reported previously. Percentage of MeHg/THg in skin tissue of CHS and IRL dolphin was about 72% and 73%, respectively. Dietary levels of trace elements may play an important role in contributing to concentration differences for As, Co, Mn, Sb, Se, THg and Tl between CHS and IRL dolphins. Total Hg concentrations were significantly correlated with the age of CHS dolphins, while an inverse relationship was detected for Cu, Mn, Pb, U and Zn. The only significant correlation found between trace element concentration and IRL dolphins' age was Mn. Geographic differences in several trace element concentrations (As, Co, Mn, Sb, Se, THg and Tl) in skin

  11. Alternative approaches to plasma confinement

    Science.gov (United States)

    Roth, J. R.

    1978-01-01

    The paper discusses 20 plasma confinement schemes each representing an alternative to the tokamak fusion reactor. Attention is given to: (1) tokamak-like devices (TORMAC, Topolotron, and the Extrap concept), (2) stellarator-like devices (Torsatron and twisted-coil stellarators), (3) mirror machines (Astron and reversed-field devices, the 2XII B experiment, laser-heated solenoids, the LITE experiment, the Kaktus-Surmac concept), (4) bumpy tori (hot electron bumpy torus, toroidal minimum-B configurations), (5) electrostatically assisted confinement (electrostatically stuffed cusps and mirrors, electrostatically assisted toroidal confinement), (6) the Migma concept, and (7) wall-confined plasmas. The plasma parameters of the devices are presented and the advantages and disadvantages of each are listed.

  12. Design and Implementation of a 200kW, 28GHz gyrotron system for the Compact Toroidal Hybrid Experiment

    Science.gov (United States)

    Hartwell, G. J.; Knowlton, S. F.; Ennis, D. A.; Maurer, D. A.; Bigelow, T.

    2016-10-01

    The Compact Toroidal Hybrid (CTH) is an l = 2 , m = 5 torsatron/tokamak hybrid (R0 = 0.75 m, ap 0.2 m, and | B | supplement the existing 15 kW klystron system operating at the fundamental frequency; the latter will be used to initially generate the plasma. Ray-tracing calculations that guide the selection of launching position, antenna focal length, and beam-steering characteristics of the ECRH have been performed with the TRAVIS code [ 1 ] . The calculated absorption is up to 95.7% for vertically propagating rays, however, the absorption is more sensitive to magnetic field variations than for a side launch where the field gradient is tokamak-like. The design of the waveguide path and components for the top-launch scenario will be presented. This work is supported by U.S. Department of Energy Grant No. DE-FG02-00ER54610.

  13. 上海市社区卫生服务影响因素的灰色关联度分析%Grey Relational Analysis on Influential Factors of Community Health Services in Shanghai

    Institute of Scientific and Technical Information of China (English)

    高艳杰; 王裕明; 刘彩云

    2012-01-01

    目的 研究上海市社区卫生服务的影响因素,找出促进上海市社区卫生服务事业进一步发展的关键点.方法 选取城镇居民家庭人均可支配收入、城镇居民消费结构(即恩格尔系数)、城镇职工基本医疗保险参保人数、社区卫生服务中心卫生技术人员数、社区卫生服务中心机构数、社区卫生服务中心床位数等6个因素,采用灰色关联度模型,分析以上6个因素对上海市社区卫生服务的影响.结果 城镇居民家庭人均可支配收入、城镇居民消费结构(即恩格尔系数)、城镇职工基本医疗保险参保人数、社区卫生服务中心卫生技术人员数、社区卫生服务中心机构数、社区卫生服务中心床位数等6个因素的关联度分别为0.7784、0.6204、0.6713、0.8249、0.6120、0.7922.结论 社区卫生服务机构卫生技术人员数对社区卫生服务的影响最大,其次是社区卫生服务中心床位数以及城镇居民家庭人均可支配收入.上海市今后应进一步加强社区卫生服务卫生技术人员的培养,特别是全科医生的培养;发展经济,深入优化医疗资源配置,推进医疗保险与社区卫生服务的接轨.%Objective To investigate the influential factors of community health services ( CHS ) in Shanghai so as to find out the key points for further development of CHS. Methods The six influential factors to community health services including per capita disposable income in urban households, consumption structure of local residents ( or Engel's coefficient), number of urban employees participating in basic medical insurance system, number of health professional personnel in CHS centers, number of CHS centers and number of beds in CHS centers were chosen and analyzed by using grey relational analysis, in order to fined out their impact on CHS in Shanghai. Results The correlation degree of the six factors were 0. 7784, 0. 6204, 0. 6713, 0. 8249 , 0. 6120, and 0. 7922

  14. 决明查尔酮合成酶基因的克隆及序列分析%Molecular Cloning and Analysis of a Chalone Synthase Gene of Cassia tora

    Institute of Scientific and Technical Information of China (English)

    廖海; 周嘉裕

    2008-01-01

    以决明(Cassia tora)为实验材料,利用RT-PCR和RACE技术,从决明嫩叶中克隆出查尔酮合成酶(Chal-one synthase,CHS)基因,其cDNA全长为1 459 bp,编码一个由390个氨基酸残基组成的多肽.氨基酸序列分析表明,决明CHS基因的氨基酸序列中含有44.61%的中性疏水氨基酸,29.74%的中性亲水氨基酸,12.56%的酸性氨基酸和13.O8%的碱性氨基酸.决明CHS基因的氨基酸序列中具有CHS家族酶系的氨基酸保守残基,包括结合底物CoA的结合残基及催化聚酮合成的催化残基,表明其可能参与聚酮化合物的合成.决明与其它植物CHS的氨基酸序列的进化分析表明,其与同为豆科决明属的翼叶决明(Cassia alata)的同源性较近,并且CHS家族可以分为CHS亚家族与非CHS亚家族.将得到的序列提交GenBank,登录号为EU430077.%Using Cassia tara as material,a cDNA encoding chalone synthase (CHS) was cloned from tender leaves of Cassia tara by RT-PCR and RACE. The full-length cDNA of CHS from Cassia tara had 1 459 bp with an open reading frame encoding 390 amino acids of protein. There were 44.61% of neutral hydrophobic residues,29.74% of neutral hydrophilic residues,12.56% of acid residues,and 13.08% of alkaline residues in the CHS amino acid sequence. It was suggested that the CHS from Cassia tara might be involved with the biosynthesis of polyketide since it maintained the same functional regions involved with CoA binding site and catalytic residues conserved in the CHS-superfamily enzymes. Furthermore, phylogenic analysis on the amino acid sequence of CHS from Cassia tara with other plants showed that Cassia tara was closely related to Cassia alata,and CHS-superfamily enzymes. They could be grouped into CHS-subfamily and non CHS-subfamily. The accession number of CHS from Cassia tara in GenBank was EU430077.

  15. Metabolite profiling of Arabidopsis thaliana (L.) plants transformed with an antisense chalcone synthase gene

    DEFF Research Database (Denmark)

    Le Gall, G.; Metzdorff, Stine Broeng; Pedersen, Jan W.;

    2005-01-01

    A metabolite profiling study has been carried out on Arabidopsis thaliana (L.) Heynh. ecotype Wassilewskija and a series of transgenic lines of the ecotype transformed with a CHS (chalcone synthase) antisense construct. Compound identifications by LC/MS and H-1 NMR are discussed. The glucosinolate...

  16. NCBI nr-aa BLAST: CBRC-PMAR-01-0383 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0383 ref|NP_001034492.1| chitin synthase 2 [Tribolium castaneum] gb|AA...Q55061.1| chitin synthase CHS2 [Tribolium castaneum] gb|AAQ62692.1| chitin synthase [Tribolium castaneum] NP_001034492.1 0.021 22% ...

  17. NCBI nr-aa BLAST: CBRC-PMAR-01-0127 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0127 ref|NP_001034492.1| chitin synthase 2 [Tribolium castaneum] gb|AA...Q55061.1| chitin synthase CHS2 [Tribolium castaneum] gb|AAQ62692.1| chitin synthase [Tribolium castaneum] NP_001034492.1 0.007 21% ...

  18. cis-regulatory elements involved in ultraviolet light regulation and plant defense.

    Science.gov (United States)

    Wingender, R; Röhrig, H; Höricke, C; Schell, J

    1990-10-01

    An elicitor-regulated transient expression system was established in soybean protoplasts that allowed the identification of cis-regulatory elements involved in plant defense. The 5' region of an ultraviolet (UV) light-inducible and elicitor-inducible chs gene (chs1) of soybean was subjected to deletion analysis with the help of chimeric chs-nptII/gus gene constructs. This analysis delimited the sequences necessary for elicitor inducibility to -175 and -134 of the chs1 promoter. The same soybean sequences were able to direct elicitor inducibility in parsley protoplasts, suggesting a conserved function of cis-acting elements involved in plant defense. In addition, this region of the soybean promoter also promotes UV light inducibility in parsley protoplasts. However, in contrast to the elicitor induction, correct regulation was not observed after UV light induction when sequences downstream of -75 were replaced by a heterologous minimal promoter. This result indicates that at least two cis-acting elements are involved in UV light induction.

  19. Department of Commerce Semiannual Regulatory Agenda

    Science.gov (United States)

    2010-04-26

    ... Part IV Department of Commerce Semiannual Regulatory Agenda ] DEPARTMENT OF COMMERCE (DOC) DEPARTMENT OF COMMERCE Office of the Secretary 13 CFR Ch. III 15 CFR Subtitle A; Subtitle B, Chs. I, II, III..., and VI Spring 2010 Semiannual Agenda of Regulations AGENCY: Office of the Secretary, Commerce....

  20. IL-1β-Dependent Activation of Dendritic Epidermal T Cells in Contact Hypersensitivity

    DEFF Research Database (Denmark)

    Nielsen, Morten M; Lovato, Paola; Macleod, Amanda S

    2014-01-01

    Substances that penetrate the skin surface can act as allergens and induce a T cell-mediated inflammatory skin disease called contact hypersensitivity (CHS). IL-17 is a key cytokine in CHS and was originally thought to be produced solely by CD4(+) T cells. However, it is now known that several cell...... in CHS. Thus, more IL-17-producing DETCs appear in the skin following exposure to DNFB in wild-type mice, and DNFB-induced ear swelling is reduced by ∼50% in TCRδ(-/-) mice compared with wild-type mice. In accordance, DNFB-induced ear swelling was reduced by ∼50% in IL-17(-/-) mice. We show that DNFB...... triggers DETC activation and IL-1β production in the skin and that keratinocytes produce IL-1β when stimulated with DNFB. We find that DETCs activated in vitro by incubation with anti-CD3 and IL-1β produce IL-17. Importantly, we demonstrate that the IL-1R antagonist anakinra significantly reduces CHS...

  1. [Cannabinoid hyperemesis syndrome: A review of the literature].

    Science.gov (United States)

    Desjardins, N; Stheneur, C

    2016-06-01

    Cannabis is the most widely used illicit drug in the world. In France, cannabis use has been increasing among youth since 2011, in both experimental use and regular consumption. A distinct syndrome, characterized by recurrent vomiting associated with abdominal pain and compulsive bathing, has been increasingly recognized in adult chronic users. Cannabinoid hyperemesis syndrome (CHS) is still underdiagnosed in adults and even more so among adolescents. Classically, CHS progresses into three distinct phases: prodromal, hyperemetic, and recovery. During the prodromal phase, the patient develops early morning nausea, a fear of vomiting, and abdominal discomfort. Afterward, the hyperemetic phase consists of incapacitating nausea and profuse vomiting. Most patients complain of mild abdominal pain and weight loss. Patients are relieved by taking hot showers. The recovery stage begins with cessation of cannabis use. The majority of patients will develop this symptom within 1-5years after the beginning of consumption. CHS is a clinical diagnosis and should be considered in every case of cyclical vomiting. To date, the specific etiology of CHS is unknown as is the pathophysiology of improvement with hot baths. All youth presenting with cyclic vomiting should be questioned about cannabis use and compulsive hot bathing. The early recognition of this syndrome will save unnecessary and invasive investigations.

  2. Effect of lead treatment on medicarpin accumulation and on the gene expression of key enzymes involved in medicarpin biosynthesis in Medicago sativa L.

    Science.gov (United States)

    Ghelich, Sima; Zarinkamar, Fatemeh; Soltani, Bahram Mohammad; Niknam, Vahid

    2014-12-01

    Lead (Pb) is the most common heavy metal contaminant in the environment. The present study was undertaken to determine the effect of Pb treatment on medicarpin production and accumulation in Medicago sativa L. To this aim, 7- and 30-day-old plants were treated with 0, 120, 240, 500, and 1,000 μM Pb during 10 days. The content of medicarpin was determined by HPLC, and the extent of medicarpin production was deduced from the result of semiquantitative RT-PCR performed on PAL, CHS, and VR genes. HPLC results indicated that medicarpin concentration has been reduced in the roots, while its exudation to the culture medium has been increased. RT-PCR results indicated that the transcript levels of PAL, CHS, and VR genes have not been affected following Pb stress in seedlings. At the vegetative stage, transcript levels of PAL and CHS genes have been reduced in the roots. However, the transcript level of VR gene increased at 120 and 240 μM Pb, while it decreased at higher concentrations. In the shoot, the transcript levels of PAL, CHS, and VR genes were increased following increased concentration of lead in the medium. Overall, q-PCR results suggest that medicarpin biosynthesis has been induced in the shoots and reduced in the roots of the plants treated with a toxic concentration of Pb.

  3. Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.

    Science.gov (United States)

    Shavandi, Amin; Bekhit, Alaa El-Din A; Ali, M Azam; Sun, Zhifa

    2015-09-01

    In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC). CHS was prepared from squid pens by alkaline N-deacetylation. HA and β-TCP were extracted from mussel shells using a microwave irradiation method. Two different composites were prepared by incorporating 50% (w/w) HA/(β-TCP) in CHS or CHC followed by lyophilization and cross-linking of composites by tripolyphosphate (TPP). The effect of different freezing temperatures of -20, -80 and -196 °C on the physicochemical characteristics of composites was investigated. A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS. Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated. The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties. The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.

  4. Altering the expression of two chitin synthase genes differentially affects the growth and morphology of Aspergillus oryzae

    DEFF Research Database (Denmark)

    Müller, Christian; Hjort, C.M.; Hansen, K.;

    2002-01-01

    abnormalities, such as ballooning cells, intrahyphal hyphae and conidial scars. The growth was severely inhibited in the presence of 10 mg CFW l(-1). In none of the constructed strains did the cell-wall composition differ from the wild-type. Northern analysis indicated no change in the transcription......, analysis of the cell-wall composition and growth in the presence of Calcofluor white (CFW). The chsB disrupted strain and the uninduced P-niiA,-ChsB strain exhibited hyperbranching, they had a lower level of conidiation than the wild-type and were sensitive to CFW at 50 mg l(-1). When chsB transcription...... was induced in the strain containing the p(niiA)-chsB construct, the strain displayed wild-type morphology on solid medium and at sub-maximum growth rates but the wild-type morphology was not fully restored during rapid growth in batch cultivation. The csmA disruption strain displayed morphological...

  5. 75 FR 20608 - Notice of Re-Designation of the Service Delivery Area for the Cowlitz Indian Tribe

    Science.gov (United States)

    2010-04-20

    ... HUMAN SERVICES Indian Health Service Notice of Re-Designation of the Service Delivery Area for the... Service Delivery Area (CHSDA) as the geographic area within which CHS will be made available by the IHS to... of Management and Budget under the Paperwork Reduction Act of 1980. Contract Health Service...

  6. 78 FR 55743 - Notice of Service Delivery Area Designation for the Shinnecock Indian Nation

    Science.gov (United States)

    2013-09-11

    ... HUMAN SERVICES Indian Health Service Notice of Service Delivery Area Designation for the Shinnecock... boundaries of the Service Delivery Area (SDA) for the newly recognized Shinnecock Indian Nation. The... Delivery Area (CHSDA), for the purposes of operating a Contract Health Service (CHS) program pursuant...

  7. Identification of LmUAP1 as a 20-hydroxyecdysone response gene in the chitin biosynthesis pathway from the migratory locust, Locusta migratoria.

    Science.gov (United States)

    Liu, Xiao-Jian; Sun, Ya-Wen; Li, Da-Qi; Li, Sheng; Ma, En-Bo; Zhang, Jian-Zhen

    2016-10-03

    In Locusta migratoria, we found that two chitin biosynthesis genes, UDP N-acetylglucosamine pyrophosphorylase gene LmUAP1 and chitin synthase gene LmCHS1, are expressed mainly in the integument and are responsible for cuticle formation. However, whether these genes are regulated by 20-hydroxyecdysone (20E) is still largely unclear. Here, we showed the developmental expression pattern of LmUAP1, LmCHS1 and the corresponding 20E titer during the last instar nymph stage of locust. RNA interference (RNAi) directed toward a common region of the two isoforms of LmEcR (LmEcRcom) reduced the expression level of LmUAP1, while there was no difference in the expression of LmCHS1. Meantime, injection of 20E in vivo induced the expression of LmUAP1 but not LmCHS1. Further, we found injection-based RNAi of LmEcRcom resulted in 100% mortality. The locusts failed to molt with no apolysis, and maintained in the nymph stage until death. In conclusion, our preliminary results indicated that LmUAP1 in the chitin biosynthesis pathway is a 20E late-response gene and LmEcR plays an essential role in locust growth and development, which could be a good potential target for RNAi-based pest control.

  8. Occurrence of triclosan in plasma of wild Atlantic bottlenose dolphins (Tursiops truncatus) and in their environment

    Energy Technology Data Exchange (ETDEWEB)

    Fair, Patricia A., E-mail: pat.fair@noaa.go [National Oceanic and Atmospheric Administration, National Ocean Services, Center for Coastal Environmental Health and Biomolecular Research, 219 Fort Johnson Road, Charleston, SC 29412-9110 (United States); Lee, H.-B. [Aquatic Ecosystem Protection Research Division, Environment Canada, 867 Lakeshore Road, Burlington, ON L7R 4A6 (Canada); Adams, Jeff [National Oceanic and Atmospheric Administration, National Ocean Services, Center for Coastal Environmental Health and Biomolecular Research, 219 Fort Johnson Road, Charleston, SC 29412-9110 (United States); Darling, Colin; Pacepavicius, Grazina; Alaee, Mehran [Aquatic Ecosystem Protection Research Division, Environment Canada, 867 Lakeshore Road, Burlington, ON L7R 4A6 (Canada); Bossart, Gregory D. [Center for Coastal Research, Marine Mammal Research and Conservation Program, Harbor Branch Oceanographic Institute at Florida Atlantic University, 5600 U.S. 1 North, Ft. Pierce, FL 34946 (United States); Henry, Natasha [National Oceanic and Atmospheric Administration, National Ocean Services, Center for Coastal Environmental Health and Biomolecular Research, 219 Fort Johnson Road, Charleston, SC 29412-9110 (United States); Muir, Derek [Aquatic Ecosystem Protection Research Division, Environment Canada, 867 Lakeshore Road, Burlington, ON L7R 4A6 (Canada)

    2009-08-15

    The presence of triclosan, a widely-used antibacterial chemical, is currently unknown in higher trophic-level species such as marine mammals. Blood plasma collected from wild bottlenose dolphins (Tursiops truncatus) in Charleston, SC (CHS) (n = 13) and Indian River Lagoon, FL (IRL) (n = 13) in 2005 was analyzed for triclosan. Plasma concentrations in CHS dolphins ranged from 0.12 to 0.27 ng/g wet weight (mean 0.18 ng/g), with 31% of the sampled individuals having detectable triclosan. The mean IRL dolphin plasma concentrations were 0.072 ng/g wet weight (range 0.025-0.11 ng/g); 23% of the samples having detectable triclosan. In the CHS area, triclosan effluent values from two WWTP were both 190 ng/L and primary influents were 2800 ng/L and 3400 ng/L. Triclosan values in CHS estuarine surface water samples averaged 7.5 ng/L (n = 18) ranging from 4.9 to 14 ng/L. This is the first study to report bioaccumulation of anthropogenic triclosan in a marine mammal highlighting the need for further monitoring and assessment. - Triclosan in bottlenose dolphin plasma and their environment.

  9. A Chinese version of the Rheumatology Attitudes Index is a valid and reliable measure of learned helplessness in patients with SLE.

    Science.gov (United States)

    Thumboo, J; Feng, P H; Chan, S P; Boey, M L; Thio, S T; Fong, K Y

    2002-01-01

    Despite the prognostic importance of learned helplessness (LH) in rheumatic diseases, there are no validated measures of LH in Chinese or other Asian languages. We therefore assessed the validity of a Chinese translation of the Rheumatology Attitudes Index (CRAI; a widely used measure of LH) and its Helplessness (CHS) and Internality (CIS) subscales in patients with SLE. Chinese-speaking SLE patients (n = 69) completed identical, self-administered questionnaires containing the CRAI and assessing demographic/socio-economic variables twice within 2 weeks. SLE activity, damage and quality of life were assessed using the BILAG, SLICC/ACR Damage Index and SF-36 respectively. Scale psychometric properties were assessed through Cronbach's alpha, intra-class correlations, quantifying test-retest differences, factor analysis and known-groups construct validity. Internal consistency and reliability were acceptable, with Cronbach's alpha for the CHS, CIS and CRAI being 0.70, 0.69 and 0.74, respectively. Mean differences in test-retest scores spanned 1.6-2.4% of possible scale ranges and intra class correlations ranged from 0.72 to 0.88. Factor analysis identified two major factors corresponding to the CHS and CIS subscales of the CRAI. Eight of 10 a priori hypotheses relating the CRAI and CHS to demographic, disease and quality of life variables were confirmed, supporting the construct validity of these scales. The CRAI and its helplessness subscale are valid and reliable measures of learned helplessness in Chinese-speaking SLE patients.

  10. Low-Latitude Coronal Holes at the Minimum of the 23rd Solar Cycle

    CERN Document Server

    Abramenko, V; Linker, J; Mikic, Z; Luhmann, J; Lee, C

    2010-01-01

    Low and mid-latitude coronal holes (CHs) observed on the Sun during the current solar activity minimum (from September 21, 2006, Carrington rotation (CR) 2048, until June 26, 2009 (CR 2084)) were analyzed using {\\it SOHO}/EIT and STEREO-A SECCHI EUVI data. From both the observations and Potential Field Source Surface (PFSS) modeling, we find that the area occupied by CHs inside a belt of $\\pm 40^\\circ$ around the solar equator is larger in the current 2007 solar minimum relative to the similar phase of the previous 1996 solar minimum. The enhanced CH area is related to a recurrent appearance of five persistent CHs, which survived during 7-27 solar rotations. Three of the CHs are of positive magnetic polarity and two are negative. The most long-lived CH was being formed during 2 days and existed for 27 rotations. This CH was associated with fast solar wind at 1 AU of approximately 620$\\pm 40$ km s$^{-1}$. The 3D MHD modeling for this time period shows an open field structure above this CH. We conclude that the...

  11. A Cooperative Harmony Search Algorithm for Function Optimization

    Directory of Open Access Journals (Sweden)

    Gang Li

    2014-01-01

    Full Text Available Harmony search algorithm (HS is a new metaheuristic algorithm which is inspired by a process involving musical improvisation. HS is a stochastic optimization technique that is similar to genetic algorithms (GAs and particle swarm optimizers (PSOs. It has been widely applied in order to solve many complex optimization problems, including continuous and discrete problems, such as structure design, and function optimization. A cooperative harmony search algorithm (CHS is developed in this paper, with cooperative behavior being employed as a significant improvement to the performance of the original algorithm. Standard HS just uses one harmony memory and all the variables of the object function are improvised within the harmony memory, while the proposed algorithm CHS uses multiple harmony memories, so that each harmony memory can optimize different components of the solution vector. The CHS was then applied to function optimization problems. The results of the experiment show that CHS is capable of finding better solutions when compared to HS and a number of other algorithms, especially in high-dimensional problems.

  12. Update of immune events in the murine contact hypersensitivity model: toward the understanding of allergic contact dermatitis.

    Science.gov (United States)

    Honda, Tetsuya; Egawa, Gyohei; Grabbe, Stephan; Kabashima, Kenji

    2013-02-01

    Allergic contact dermatitis (ACD) is one of the most common skin diseases, consisting of sensitization and elicitation phases. With the advancement of technology and the discovery of new types of immune cells, our knowledge of the immunological mechanisms of contact hypersensitivity (CHS) as a murine model of ACD has expanded significantly in the past decade. For example, by introducing regulatory T cells, CD4(+) T-helper 17 cells, and Langerin-positive dermal dendritic cells, the initiation and termination mechanism of CHS has been revealed. In addition, the role of mast cells in CHS, long a matter of debate, has become apparent by developing conditional mast cell-deficient mice. Moreover, the role of the innate immunity system, such as that of Toll-like receptor signaling, has made a breakthrough in this field. In this review, we will integrate the recent advancement of immunological mechanisms of both the sensitization and elicitation phases of CHS into the classic view, and we will discuss updated mechanisms on its development and future directions.

  13. Immobilized Lentivirus Vector on Chondroitin Sulfate-Hyaluronate Acid-Silk Fibroin Hybrid Scaffold for Tissue-Engineered Ligament-Bone Junction

    Directory of Open Access Journals (Sweden)

    Liguo Sun

    2014-01-01

    Full Text Available The lack of a fibrocartilage layer between graft and bone remains the leading cause of graft failure after anterior cruciate ligament (ACL reconstruction. The objective of this study was to develop a gene-modified silk cable-reinforced chondroitin sulfate-hyaluronate acid-silk fibroin (CHS hybrid scaffold for reconstructing the fibrocartilage layer. The scaffold was fabricated by lyophilizing the CHS mixture with braided silk cables. The scanning electronic microscopy (SEM showed that microporous CHS sponges were formed around silk cables. Each end of scaffold was modified with lentiviral-mediated transforming growth factor-β3 (TGF-β3 gene. The cells on scaffold were transfected by bonded lentivirus. In vitro culture demonstrated that mesenchymal stem cells (MSCs on scaffolds proliferated vigorously and produced abundant collagen. The transcription levels of cartilage-specific genes also increased with culture time. After 2 weeks, the MSCs were distributed uniformly throughout scaffold. Deposited collagen was also found to increase. The chondral differentiation of MSCs was verified by expressions of collagen II and TGF-β3 genes in mRNA and protein level. Histology also confirmed the production of cartilage extracellular matrix (ECM components. The results demonstrated that gene-modified silk cable-reinforced CHS scaffold was capable of supporting cell proliferation and differentiation to reconstruct the cartilage layer of interface.

  14. Glyceollin is an important component of soybean plant defense against Phytophthora sojae and Macrophomina phaseolina

    Science.gov (United States)

    Transgenic soybean plants were generated using bombardment of embryogenic cultures with the PAL5 (phenylalanine ammonia lyase), CHS6 (chalcone synthase) and IFS2 (isoflavone synthase) genes in sense orientation, driven by the cotyledon-preferable lectin promoter, or with the IFS2 (isoflavone synthas...

  15. Transient silencing of CHALCONE SYNTHASE during fruit ripening modifies tomato epidermal cells and cuticle properties.

    Science.gov (United States)

    España, Laura; Heredia-Guerrero, José A; Reina-Pinto, José J; Fernández-Muñoz, Rafael; Heredia, Antonio; Domínguez, Eva

    2014-11-01

    Tomato (Solanum lycopersicum) fruit ripening is accompanied by an increase in CHALCONE SYNTHASE (CHS) activity and flavonoid biosynthesis. Flavonoids accumulate in the cuticle, giving its characteristic orange color that contributes to the eventual red color of the ripe fruit. Using virus-induced gene silencing in fruits, we have down-regulated the expression of SlCHS during ripening and compared the cuticles derived from silenced and nonsilenced regions. Silenced regions showed a pink color due to the lack of flavonoids incorporated to the cuticle. This change in color was accompanied by several other changes in the cuticle and epidermis. The epidermal cells displayed a decreased tangential cell width; a decrease in the amount of cuticle and its main components, cutin and polysaccharides, was also observed. Flavonoids dramatically altered the cuticle biomechanical properties by stiffening the elastic and viscoelastic phase and by reducing the ability of the cuticle to deform. There seemed to be a negative relation between SlCHS expression and wax accumulation during ripening that could be related to the decreased cuticle permeability to water observed in the regions silencing SlCHS. A reduction in the overall number of ester linkages present in the cutin matrix was also dependent on the presence of flavonoids.

  16. Synthesis and biological evaluation of novel phosphoramidate derivatives of coumarin as chitin synthase inhibitors and antifungal agents.

    Science.gov (United States)

    Ji, Qinggang; Ge, Zhiqiang; Ge, Zhixing; Chen, Kaizhi; Wu, Hualong; Liu, Xiaofei; Huang, Yanrong; Yuan, Lvjiang; Yang, Xiaolan; Liao, Fei

    2016-01-27

    A series of novel phosphoramidate derivatives of coumarin have been designed and synthesized as chitin synthase (CHS) inhibitors. All the synthesized compounds have been screened for their chitin synthase inhibition activity and antimicrobial activity in vitro. The bioactive assay manifested that most of the target compounds exhibited good efficacy against CHS and a variety of clinically important fungal pathogens. In particular, compound 7t with IC50 of 0.08 mM against CHS displayed stronger efficiency than the reference Polyoxin B with IC50 of 0.16 mM. In addition, the apparent Ki values of compound 7t was 0.096 mM while the Km of Chitin synthase prepared from Candida tropicalis was 3.86 mM for UDP-N-acetylglucosamine, and the result of the Ki showed that the compounds was a non-competitive inhibitor of the CHS. As far as the antifungal activity is concerned, compounds 7o, 7r and 7t were highly active against Aspergillus flavus with MIC values in the range of 1 μg/mL to 2 μg/Ml while the results of antibacterial screening showed that these compounds have negligible actions to the tested bacteria. These results indicated that the design of these compounds as antifungal agents was rational.

  17. Department of Agriculture Semiannual Regulatory Agenda

    Science.gov (United States)

    2010-04-26

    ... Part III Department of Agriculture Semiannual Regulatory Agenda ] DEPARTMENT OF AGRICULTURE (USDA) DEPARTMENT OF AGRICULTURE Office of the Secretary 7 CFR Subtitle A, Chs. I-VII, IX-XII, XIV-XVIII, XXI, XXIV.... Department of Agriculture (USDA) in conformance with Executive Order 12866 ``Regulatory Planning and...

  18. AcEST: DK962938 [AcEST

    Lifescience Database Archive (English)

    Full Text Available _SOYBN Chalcone synthase 1 OS=Glycine max GN=CHS1 ... 252 1e-66 sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria...GAD+ LTKLLGLRPSV Sbjct: 128 IFCTTSGVDMPGADYQLTKLLGLRPSV 154 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria

  19. AcEST: DK957747 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 61 2e-69 sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS... 250 4e-66 sp|P49440|CHSY_PHAVU ...TKLLGLRP 631 +FCTTSGVDMPGADWALTKLLGLRP Sbjct: 134 VFCTTSGVDMPGADWALTKLLGLRP 158 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria

  20. AcEST: DK962141 [AcEST

    Lifescience Database Archive (English)

    Full Text Available synthase 1 OS=Glycine max GN=CHS1 ... 262 1e-69 sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata G...KLLGLRPSVKR MMY Sbjct: 128 IFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMY 160 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria

  1. AcEST: DK955505 [AcEST

    Lifescience Database Archive (English)

    Full Text Available p|P30074|CHS2_MEDSA Chalcone synthase 2 OS=Medicago sativa GN=C... 192 3e-49 sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria...ery: 363 RQVLSDYGNMSSACVLF 413 R+VLS+YGNMSSACVLF Sbjct: 328 REVLSEYGNMSSACVLF 344 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria

  2. AcEST: DK961880 [AcEST

    Lifescience Database Archive (English)

    Full Text Available |P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS... 244 3e-64 sp|P49440|CHSY_PHAVU Chalcone sy...FCTTSGVDMPGADWALTKLLG Sbjct: 134 VFCTTSGVDMPGADWALTKLLG 155 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria

  3. AcEST: DK955574 [AcEST

    Lifescience Database Archive (English)

    Full Text Available setum arvense GN=C... 175 2e-43 sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria...-QPKSK 129 >sp|P23569|CHSY_PUELO Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 SV=1 Length = 389 Score =

  4. Users' Satisfaction with Library Information Resources and Services: A Case Study College of Health Sciences Library Niger Delta University, Amassoma, Nigeria

    Science.gov (United States)

    Tiemo, Pereware Aghwotu; Ateboh, Benedict Alaowei

    2016-01-01

    This study investigated users' satisfaction with library information resources and services at the College of Health Sciences (CHS) library Niger Delta University, Nigeria. The objective was to determine the level of users satisfaction with library information resources and services. 2 (two) research questions were formulated to guide the study.…

  5. Cellular target of UVB-induced DNA damage resulting in local suppression of contact hypersensitivity

    NARCIS (Netherlands)

    Vink, A.A.; Shreedhar, V.; Roza, L.; Krutmann, J.; Kripke, M.L.

    1998-01-01

    Experimental data are reviewed that lend support to the hypothesis that formation of DNA damage is the initiation event of local suppression of contact hypersensitivity (CHS) after exposure to ultraviolet (UV) radiaton and that the antigen-presenting cell (APC) is an important traget for this DNA da

  6. Management Advisory Services by CPA’s. A Survey of Current Expansion of Sole Proprietor Professional Practice Units.

    Science.gov (United States)

    1981-12-01

    text. tial andl existing managerial talent; of National Affains. executive responibility desuiphios: 1974). Chs 1.2,14.15. ree of ex~ecutive performance...W.; Laurence , Paul R.; and Lorsch, Jay W. Organizational Structure and Desin. Homewood, IL: Irwin, 1970. Drucker, Peter F. Managing for Results

  7. Efecto de la Adición de Minerales en el Agua o Alimento sobre la Frecuencia Cardiaca, en Pollos de Engorde Sometidos a Estrés Calórico Crónico y Agudo

    DEFF Research Database (Denmark)

    Rojas, Jesus; Steffensen, Simon Gabriel Comerma; Chacon, Tony

    2008-01-01

    The effects of mineral addition in feed or water on performance parameters and heart rate (HR) were evaluated in broilers at 28-35 d and 36 d under both chronic heat stress (CHS) and acute heat stress (AHS) in laboratory conditions. Onehundred ninety two broilers were used, distributed in 6 cages...

  8. Liquid Silane Routes to Electronic Materials

    Energy Technology Data Exchange (ETDEWEB)

    Douglas L. Schulz; Xuliang Dai; Kendric J. Nelson; Konstantin Pokhodnya; Justin M. Hoey; Iskander S. Akhatov; Orven F. Swenson; Jeremiah Smith; John Lovaasen; Matt Robinson; Scott Payne; Philip R. Boudjouk

    2008-12-04

    New chemistries based upon liquid cyclohexasilane (Si{sub 6}H{sub 12} or CHS) have been used as precursors to silicon-containing electronic materials. Spin-coating of CHS-based inks with subsequent UV light and/or thermal treatment yielded amorphous silicon (a-Si:H) films. While initial ink chemistries gave a-Si:H with high resistivity (i.e., > 10{sup 6} {Omega}.cm), several doping strategies are under development to address this limitation. In this contribution, the current status of solution processed rectifying diodes and field effect transistors fabricated from CHS-based inks will be presented. Additionally, a new printing approach termed collimated aerosol beam direct write (CAB-DW{sup TM}) was developed that allows the deposition of printed Ag lines 5 {mu}m in width. A status update will be given where CHS-based inks have been used to CAB-DW silicon-based features with linewidths <10 {mu}m. Assuming silicon thin film materials with good electrical properties will be developed, there may be significant cost advantages associated with the ability to controllably deposit the semiconductor in a metered fashion.

  9. Metabolic Response of Human Osteoarthritic Cartilage to Biochemically Characterized Collagen Hydrolysates

    Directory of Open Access Journals (Sweden)

    Saskia Schadow

    2017-01-01

    Full Text Available The most frequent disease of the locomotor system is osteoarthritis (OA, which, as a chronic joint disease, might benefit more from nutrition than acute illnesses. Collagen hydrolysates (CHs are peptidic mixtures that are often used as nutraceuticals for OA. Three CHs were characterized biochemically and pharmacologically. Our biophysical (MALDI-TOF-MS, NMR, AFM and fluorescence assays revealed marked differences between CHs of fish (Peptan® F 5000, Peptan® F 2000 and porcine (Mobiforte® origin with respect to the total number of peptides and common peptides between them. Using a novel dual radiolabeling procedure, no CH modulated collagen biosynthesis in human knee cartilage explants. Peptan® F 2000 enhanced the activities of the aggrecanase ADMATS4 and ADMATS5 in vitro without loss of proteoglycan from cartilage explants; the opposite effect was observed with Mobiforte®. Interleukin (IL-6, matrix metalloproteinase (MMP-1, -3 and -13 levels were elevated in explants that were treated with Mobiforte® and Peptan® F 5000, but not with Peptan® F 2000. In conclusion, the heterogeneous peptide composition and disparate pharmacological effects between CHs suggest that the effect of a CH preparation cannot be extrapolated to other formulations. Thus, the declaration of a CH as a safe and effective nutraceutical requires a thorough examination of its pleiotropic effects.

  10. Metabolic Response of Human Osteoarthritic Cartilage to Biochemically Characterized Collagen Hydrolysates

    Science.gov (United States)

    Schadow, Saskia; Simons, Viktor S.; Lochnit, Guenter; Kordelle, Jens; Gazova, Zuzana; Siebert, Hans-Christian; Steinmeyer, Juergen

    2017-01-01

    The most frequent disease of the locomotor system is osteoarthritis (OA), which, as a chronic joint disease, might benefit more from nutrition than acute illnesses. Collagen hydrolysates (CHs) are peptidic mixtures that are often used as nutraceuticals for OA. Three CHs were characterized biochemically and pharmacologically. Our biophysical (MALDI-TOF-MS, NMR, AFM) and fluorescence assays revealed marked differences between CHs of fish (Peptan® F 5000, Peptan® F 2000) and porcine (Mobiforte®) origin with respect to the total number of peptides and common peptides between them. Using a novel dual radiolabeling procedure, no CH modulated collagen biosynthesis in human knee cartilage explants. Peptan® F 2000 enhanced the activities of the aggrecanase ADMATS4 and ADMATS5 in vitro without loss of proteoglycan from cartilage explants; the opposite effect was observed with Mobiforte®. Interleukin (IL)-6, matrix metalloproteinase (MMP)-1, -3 and -13 levels were elevated in explants that were treated with Mobiforte® and Peptan® F 5000, but not with Peptan® F 2000. In conclusion, the heterogeneous peptide composition and disparate pharmacological effects between CHs suggest that the effect of a CH preparation cannot be extrapolated to other formulations. Thus, the declaration of a CH as a safe and effective nutraceutical requires a thorough examination of its pleiotropic effects. PMID:28117674

  11. Occurrence of triclosan in plasma of wild Atlantic bottlenose dolphins (Tursiops truncatus) and in their environment.

    Science.gov (United States)

    Fair, Patricia A; Lee, Hing-Biu; Adams, Jeff; Darling, Colin; Pacepavicius, Grazina; Alaee, Mehran; Bossart, Gregory D; Henry, Natasha; Muir, Derek

    2009-01-01

    The presence of triclosan, a widely-used antibacterial chemical, is currently unknown in higher trophic-level species such as marine mammals. Blood plasma collected from wild bottlenose dolphins (Tursiops truncatus) in Charleston, SC (CHS) (n = 13) and Indian River Lagoon, FL (IRL) (n = 13) in 2005 was analyzed for triclosan. Plasma concentrations in CHS dolphins ranged from 0.12 to 0.27 ng/g wet weight (mean 0.18 ng/g), with 31% of the sampled individuals having detectable triclosan. The mean IRL dolphin plasma concentrations were 0.072 ng/g wet weight (range 0.025-0.11 ng/g); 23% of the samples having detectable triclosan. In the CHS area, triclosan effluent values from two WWTP were both 190 ng/L and primary influents were 2800 ng/L and 3400 ng/L. Triclosan values in CHS estuarine surface water samples averaged 7.5 ng/L (n = 18) ranging from 4.9 to 14 ng/L. This is the first study to report bioaccumulation of anthropogenic triclosan in a marine mammal highlighting the need for further monitoring and assessment.

  12. Contact sensitizers induce skin inflammation via ROS production and hyaluronic acid degradation.

    Directory of Open Access Journals (Sweden)

    Philipp R Esser

    Full Text Available BACKGROUND: Allergic contact dermatitis (ACD represents a severe health problem with increasing worldwide prevalence. It is a T cell-mediated skin disease induced by protein-reactive organic and inorganic chemicals. A key feature of contact allergens is their ability to trigger an innate immune response that leads to skin inflammation. Previous evidence from the mouse contact hypersensitivity (CHS model suggests a role for endogenous activators of innate immune signaling. Here, we analyzed the role of contact sensitizer induced ROS production and concomitant changes in hyaluronic acid metabolism on CHS responses. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed in vitro and in vivo ROS production using fluorescent ROS detection reagents. HA fragmentation was determined by gel electrophoresis. The influence of blocking ROS production and HA degradation by antioxidants, hyaluronidase-inhibitor or p38 MAPK inhibitor was analyzed in the murine CHS model. Here, we demonstrate that organic contact sensitizers induce production of reactive oxygen species (ROS and a concomitant breakdown of the extracellular matrix (ECM component hyaluronic acid (HA to pro-inflammatory low molecular weight fragments in the skin. Importantly, inhibition of either ROS-mediated or enzymatic HA breakdown prevents sensitization as well as elicitation of CHS. CONCLUSIONS/SIGNIFICANCE: These data identify an indirect mechanism of contact sensitizer induced innate inflammatory signaling involving the breakdown of the ECM and generation of endogenous danger signals. Our findings suggest a beneficial role for anti-oxidants and hyaluronidase inhibitors in prevention and treatment of ACD.

  13. Energetic Particle Diagnostics for Transport Analysis 3.Escaping Fast Ion Diagnostics for the Fast Particle Transport Analysis

    Science.gov (United States)

    Isobe, Mitsutaka; Shinohara, Kouji

    Escaping energetic ion diagnostics in magnetically confined plasma experiments are described in this lecture note. Experimental results from escaping energetic ion diagnostics in TFTR, JFT-2M, CHS and W7-AS are shown. In addition to mechanism of energetic ion loss from a viewpoint of particle orbit, effect of MHD activity on energetic particle transport is reviewed.

  14. Effect of ascorbic acid or increasing metabolizable energy level with or without supplementation of some essential amino acids on productive and physiological traits of slow-growing chicks exposed to chronic heat stress.

    Science.gov (United States)

    Attia, Y A; Hassan, R A; Tag El-Din, A E; Abou-Shehema, B M

    2011-12-01

    Four hundred and twenty, 21-day-old slow-growing chicks were divided randomly into seven treatments, each containing five replicates. Each replicate was kept in a 1 × 1-m floor pen. One treatment was kept under thermo-neutral conditions in a semi-open house and fed a corn-soybean meal diet (positive control). The other six groups were kept under chronic heat stress (CHS) at 38 °C and 60% RH for 4 h from 12:00 to 16:00 pm for three successive days per week. Chicks in CHS treatments were fed a corn-soybean meal diet without (negative control) or with increasing metabolizable energy (ME) level by oil supplementation alone, or also with increasing some essential amino acids (EAA) such as methionine (Met), methionine and lysine (Met+Lys) or methionine, lysine and arginine (Met+Lys+Arg) or supplemented with 250 mg of ascorbic acid (AA)/kg. CHS impaired (p < 0.05) growth performance, increased plasma triglycerides and total serum Ca while decreasing (p < 0.05) plasma glucose and total serum protein. Meanwhile 250 mg AA/kg diet or an increasing ME without or with some EAA partially alleviated (p < 0.0001) the negative effect of CHS on growth while increasing (p < 0.05) feed intake and improving (p < 0.05) feed:gain ratio (F:G) and crude protein (CP) digestibility (p < 0.05). AA or increasing ME with or without EAA increased (p < 0.05) percentage dressing, liver and giblets to those of the positive control. AA or increasing ME with or without EAA partially alleviated the negative effect of CHS on blood pH, packed cell volume (PCV), haemoglobin (Hgb), total serum protein and total Ca, plasma glucose and triglyceride, rectal temperature and respiration rate. Increasing ME level improved chickens' tolerance to CHS without a significant difference from those supplemented with AA. However, increasing Met, Lys and Arg concentration did not improve performance over that recorded with increasing ME level alone. Under CHS, 250 mg AA/kg diet or increasing ME level by addition of 3

  15. Investigation of a miRNA-Induced Gene Silencing Technique in Petunia Reveals Alterations in miR173 Precursor Processing and the Accumulation of Secondary siRNAs from Endogenous Genes.

    Directory of Open Access Journals (Sweden)

    Yao Han

    Full Text Available MIGS (miRNA-induced gene silencing is a straightforward and efficient gene silencing technique in Arabidopsis. It works by exploiting miR173 to trigger the production of phasiRNAs (phased small interfering RNAs. MIGS can be used in plant species other than Arabidopsis by co-expression of miR173 and target gene fragments fused to an upstream miR173 target site. However, the efficiency and technical mechanisms have not been thoroughly investigated in other plants. In this work, two vectors, pMIGS-chs and pMIGS-pds, were constructed and transformed into petunia plants. The transgenic plants showed CHS (chalcone synthase and PDS (phytoene desaturase gene-silencing phenotypes respectively, indicating that MIGS functions in petunia. MIGS-chs plants were used to investigate the mechanisms of this technique in petunia. Results of 5'- RACE showed that the miR173 target site was cleaved at the expected position and that endogenous CHS genes were cut at multiple positions. Small RNA deep sequencing analysis showed that the processing of Arabidopsis miR173 precursors in MIGS-chs transgenic petunia plants did not occur in exactly the same way as in Arabidopsis, suggesting differences in the machinery of miRNA processing between plant species. Small RNAs in-phase with the miR173 cleavage register were produced immediately downstream from the cleavage site and out-of-phase small RNAs were accumulated at relatively high levels from processing cycle 5 onwards. Secondary siRNAs were generated from multiple sites of endogenous CHS-A and CHS-J genes, indicating that miR173 cleavage induced siRNAs have the same ability to initiate siRNA transitivity as the siRNAs functioning in co-suppression and hpRNA silencing. On account of the simplicity of vector construction and the transitive amplification of signals from endogenous transcripts, MIGS is a good alternative gene silencing method for plants, especially for silencing a cluster of homologous genes with redundant

  16. Cloning and Sequence Analyzing of Chalcone Synthase Gene in Loropetalum chinense var.Rubrum%红花檵木CHS基因的克隆与序列分析

    Institute of Scientific and Technical Information of China (English)

    许威; 于晓英; 陈己任; 符红艳; 胡博文; 陈彦斌; 李达

    2013-01-01

    查尔酮合酶(chalcone synthase,CHS)是进入类黄酮和花色素苷次生代谢的第1个关键酶.根据植物查尔酮合成酶保守区序列设计引物,以红花檵木Loropetalurn chinense var.Rubrum)大叶红的嫩叶为材料,用RT-PCR方法,分离得到了一个查尔酮合成酶基因的eDNA(GenBank登录号为JQ609678),将该基因命名为Lc vrCHS1.该序列长927 bp,编码232个氨基酸残基.其核苷酸序列与GenBank已登录的同样来源的核桃、山茶属植物CHS序列同源性达83%,与其他科植物(绣球花、葡萄、桃、马铃薯、甘草、领春木属)CHS序列同源性也达到80%以上;其编码的氨基酸序列与山茶属、葡萄、鳄梨、洋梨、沙梨、映山红CHS基因编码的氨基酸序列同样具有高度同源性,同源性高达98%.%Chalcone synthase (chalcone synthase, CHS) is the key enzyme that catalyzes the first step in flavonoids biosynthesis and anthocyanins secondary metabolites. A full-length cDNA encoding CHS was cloned from the young leaves of Loropetalurn chinense var. rubrum by RT-PCR using specific primers based on the highly conserved sequences of plant CHS that had already known. Blast search revealed that it was a new gene, and was named as LcvrCHSl (GenBank accession: JQ609678). The sequence was 927 bp, encoding 232 amino acid residues. It had 83% sequence homology with walnut and camellia that had been logged in GenBank; with other genus plants (hydrangea, grapes, peaches, potatoes, licorice, Euptelea genus), CHS sequence homology was also more than 80%; with other plants (camellia, grapes, avocados, bartlett pear, sand pear, azalea), CHS sequence also had high homology, up to 98% homology.

  17. Analysis on System Archetype of Developing Community Health Services:Based on A Field Research in Jiangxi Province%基于江西省社区卫生服务中心实地调研的社区卫生服务发展系统基模分析

    Institute of Scientific and Technical Information of China (English)

    李丽清; 周小军

    2013-01-01

    社区卫生服务健康、有序的发展能够有效缓解"看病难、看病贵"的问题.本研究从系统工程的角度对江西省城市社区卫生服务发展状况进行分析,在系统调研的基础上,以江西省南昌市、九江市、赣州市作为研究对象,将社区卫生服务机构的实际运行情况与管理理论紧密结合,深入系统地分析制约社区卫生服务发展的限制因素.并借助系统动力学反馈动态复杂原理及彼得·圣吉的系统基模分析技术构建了制约社区卫生服务发展的典型系统基模,分别对此进行了定性分析,提出了有针对性的发展策略,为提升社区卫生服务能力提供了理论依据.%The development of community health services ( CHS ) can effectively alleviate the problems of " difficulty to see a doctor and higher medical expense" . From the perspective of system engineering, we analyze in this paper the developing situation of CHS in urban areas of Nanchang, Jiujiang and Ganzhou cities in Jiangxi Province, taking the conditions of actual operation of CHS institutions in close connection with management theory, finding out the negative factors restricting the development of CHS. According to the theory of system dynamics and the analyzing technique of Peter Senge' system archetype, we build up a typical system archetype restricting development of CHS, and analyze it quantitatively, and then we propose the targeted strategy to improve the development of CHS, and thus we provide a theoretical basis for increasing service ability in CHS.

  18. 苦荞查尔酮合成酶基因序列特征及分子进化分析%Characterize of Sequences and Molecular Evolution Analysis of Chalcone Synthase in Fagopyrum Tartaricum

    Institute of Scientific and Technical Information of China (English)

    孙朝霞; 侯思宇; 郭彬; 令狐斌; 黄可盛; 许冬梅; 韩渊怀

    2014-01-01

    Compared to other grain crops, Fagopyrum tataricum contain high levels of flavonoids, especially rutin. It has important significance for the research of key enzymes in the rutin biosynthesis pathway. This study focuses on the rutin biosynthesis pathway first key gene-chalcone synthase. Two genes FtCHS1 (1 430 bp, Genbank number:KJ130961) and FtCHS2 (1 454 bp, Gnebank number: KJ139980) were assembing and sequenced according to flo-wer transcriptom in F. tataricum. Protein 3D structure domain analysis showed that FtCHS contains three active site, 11 product binding sites, five acetyl coenzyme A binding sites. The amino acid sequences were aligned and compared to orthologous sequences from other plants, such as F. esculentum, F. dibotrys and P. cuspidatum. The phylogenetic tree showed that 22 polygonaceae plants was divided into two categories: (Ⅰ and Ⅱ), FtCHS1 and FtCHS2 belong to different categories. Gene expression of different tissues for FtCHS were analysed by semi-quantitive and real-time PCR. It showed that it had a higher expression level in immature embryo and flower for FtCHS1 and FtCHS2, and the expression level were decreased in roots and stems. Our work provides a strategy to improve rutin content, which could also be used to study molecular mechanism of rutin biosynthesis pathway.%相比其他杂粮作物,苦荞(Fagopyrum tataricum)植株中含有高含量的黄酮类物质--‘芦丁’,因此对其生物合成途径中关键酶的研究具有十分重要意义。查尔酮合成酶(chalcone synthase)是黄酮类物质生物合成的第一个关键酶,本研究基于转录组数据库同源搜索方法,核酸序列拼接获得两个查尔酮合成酶基因,分别命名为 FtCHS1(Genbank 登录号: KJ130961)和 FtCHS2(Genbank 登录号: KJ139980)。生物信息学分析结果表明,这两个基因编码的蛋白序列有3个保守结构域,其结构域上包含3个活性位点,11个产物结合位点,5个乙酰辅酶 A 结合

  19. Time courses for phytochrome-induced enzyme levels in phenylpropanoid metabolism (phenylalanine ammonia-lyase, naringenin-chalcone synthase) compared with time courses for phytochrome-mediated end-product accumulation (anthocyanin, quercetin).

    Science.gov (United States)

    Brödenfeldt, R; Mohr, H

    1988-12-01

    Time course for changes in the levels of enzymes characteristic of general phenylpropanoid metabolism (phenylalanine ammonia-lyase, PAL; EC 4.3.1.5) and of the flavonoid-glycoside branch pathway (naringenin-chalcone synthase, CHS; EC 2.3.1.74) were measured in the cotyledons of mustard (Sinapis alba L.) seedlings and compared with the rates of accumulation of related end products (anthocyanin and quercetin). Induction of enzyme levels and of end-product accumulation was carried out with red and far-red (FR) light, operating via phytochrome. The data are compatible with the concept that the phytochrome-mediated appearance of enzymes such as PAL and CHS is indeed a prerequisite for the appearance of anthocyanins and flavonols. However, there is no close correlation between enzyme levels and the rates of synthesis of end products which could justify the identification of specific rate-limiting enzymes. Rather, the data indicate that there is a second phytochrome-dependent step, beyond enzyme induction, where the actual rate of flavonoid accumulation is determined. Anthocyanin and quercetin accumulation respond differently to light. However, the relative action of continuous FR, red light pulses and 'stored phytochrome signal' is the same in both cases. This indicates that the mode of operation of phytochrome is the same in both cases. The two syntheses differ only in the degree of responsiveness towards phytochrome. The time course for changes in CHS levels in continuous FR, i.e. under conditions of phytochrome photosteady state, is similar to the time course for PAL levels whereas the time courses in darkness, following transfer from FR to darkness, are totally different. In the case of CHS, a transient rise is observed whereas, with PAL, an instantaneous drop in enzyme level occurs after transfer from FR to darkness. It is concluded that the 'stored phytochrome signal' operates in darkness in the case of CHS but not in the case of PAL.

  20. Pannexin Channels Mediate the Acquisition of Myogenic Commitment in C2C12 Reserve Cells Promoted by P2 Receptor Activation

    Directory of Open Access Journals (Sweden)

    Manuel Antonio Riquelme

    2015-05-01

    Full Text Available The acquisition of myoblast commitment to the myogenic linage requires rises in intracellular free Ca2+ concentration ([Ca2+]i. Putative cell membrane pathways involved in these [Ca2+]i increments are P2 receptors (P2Rs as well as connexin (Cx and/or pannexin (Panx hemichannels and channels (Cx HChs and Panx Chs, respectively, which are known to permeate Ca2+. Reserve cells (RCs are uncommitted myoblasts obtained from differentiated C2C12 cell cultures, which acquire commitment upon replating. Regarding these cells, we found that extracellular ATP increases the [Ca2+]i via P2Rs. Moreover, ATP increases the plasma membrane permeability to small molecules and a non-selective membrane current, both of which were inhibited by Cx HCh/Panx1Ch blockers. However, RCs exposed to divalent cation-free saline solution, which is known to activate Cx HChs (but not Panx Chs, did not enhance membrane permeability, thus ruling out the possible involvement of Cx HChs. Moreover, ATP-induced membrane permeability was inhibited with blockers of P2Rs that activate Panx Chs. In addition, exogenous ATP induced the expression of myogenic commitment and increased MyoD levels, which was prevented by the inhibition of P2Rs or knockdown of Panx1 Chs. Similarly, increases in MyoD levels induced by ATP released by RCs were inhibited by Panx Ch/Cx HCh blockers. Myogenic commitment acquisition thus requires a feed-forward mechanism mediated by extracellular ATP, P2Rs and Panx Chs.

  1. Novel targeting using nanoparticles: an approach to the development of an effective anti-leishmanial drug-delivery system.

    Science.gov (United States)

    Ribeiro, Tatiana G; Chávez-Fumagalli, Miguel A; Valadares, Diogo G; França, Juçara R; Rodrigues, Lívia B; Duarte, Mariana C; Lage, Paula S; Andrade, Pedro H R; Lage, Daniela P; Arruda, Leonardo V; Abánades, Daniel R; Costa, Lourena E; Martins, Vivian T; Tavares, Carlos A P; Castilho, Rachel O; Coelho, Eduardo A F; Faraco, André A G

    2014-01-01

    The study reported here aimed to develop an optimized nanoparticle delivery system for amphotericin B (AmpB) using a polyelectrolyte complexation technique. For this, two oppositely charged polymers presenting anti-leishmanial activity - chitosan (Cs) and chondroitin sulfate (ChS) - were used: Cs as a positively charged polymer and ChS as a negatively charged polymer. The chitosan (NQ) nanoparticles, chitosan-chondroitin sulfate (NQC) nanoparticles, and chitosan-chondroitin sulfate-amphotericin B (NQC-AmpB) nanoparticles presented a mean particle size of 79, 104, and 136 nm, respectively; and a polydispersity index of 0.2. The measured zeta potential of the nanoparticles indicated a positive charge in their surface, while scanning and transmission electron microscopy revealed spherical nanoparticles with a smooth surface. Attenuated total reflectance-Fourier transform infrared spectroscopy analysis showed an electrostatic interaction between the polymers, whereas the release profile of AmpB from the NQC-AmpB nanoparticles showed a controlled release. In addition, the Cs; ChS; and NQ, NQC, and NQC-AmpB nanoparticles proved to be effective against promastigotes of Leishmania amazonensis and Leishmania chagasi, with a synergistic effect observed between Cs and ChS. Moreover, the applied NQ, NQC, and NQC-AmpB compounds demonstrated low toxicity in murine macrophages, as well as null hemolytic activity in type O(+) human red blood cells. Pure AmpB demonstrated high toxicity in the macrophages. The results show that cells infected with L. amazonensis and later treated with Cs, ChS, NQ, NQC, NQC-AmpB nanoparticles, or pure AmpB presented with a significant reduction in parasite number in the order of 24%, 31%, 55%, 66%, 90%, and 89%, respectively. The data presented indicate that the engineered NQC-AmpB nanoparticles could potentially be used as an alternative therapy to treat leishmaniasis, mainly due its low toxicity to mammals' cells.

  2. Cytolocalization of the class V chitin synthase in the yeast, hyphal and sclerotic morphotypes of Wangiella (Exophiala) dermatitidis.

    Science.gov (United States)

    Abramczyk, Dariusz; Park, Changwon; Szaniszlo, Paul J

    2009-01-01

    Wangiella (Exophiala) dermatitidis is a polymorphic fungus that produces polarized yeast and hyphae, as well as a number of non-polarized sclerotic morphotypes. The phenotypic malleability of this agent of human phaeohyphomycosis allows detailed study of its biology, virulence and the regulatory mechanisms responsible for the transitions among the morphotypes. Our prior studies have demonstrated the existence of seven chitin synthase structural genes in W. dermatitidis, each of which encodes an isoenzyme of a different class. Among them, the class V chitin synthase (WdChs5p) is most unique in terms of protein structure, because it has an N-terminal myosin motor-like domain with a P-loop (MMD) fused to its C-terminal chitin synthase catalytic domain (CSCD). However, the exact role played by WdChs5p in the different morphotypes remains undefined beyond the knowledge that it is the only single chitin synthase required for sustained cell growth at 37 degrees C and consequently virulence. This report describes the expression in Escherichia coli of a 12kDa polypeptide (WdMyo12p) of WdChs5p, which was used to raise in rabbits a polyclonal antibody that recognized exclusively its MMD region. Results from the use of the antibody in immunocytolocalization studies supported our previous findings that WdChs5p is critically important at infection temperatures for maintaining the cell wall integrity of developing yeast buds, elongating tips of hyphae, and random sites of expansion in sclerotic forms. The results also suggested that WdChs5p localizes to the regions of cell wall growth in an actin-dependent fashion.

  3. Preparation and characterisation of irradiated crab chitosan and New Zealand Arrow squid pen chitosan

    Energy Technology Data Exchange (ETDEWEB)

    Shavandi, Amin, E-mail: amin.shavandi@postgrad.otago.ac.nz [Department of Food Sciences, University of Otago, Dunedin (New Zealand); Bekhit, Adnan A. [Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Alexandria, Alexandria (Egypt); Bekhit, Alaa El-Din A. [Department of Food Sciences, University of Otago, Dunedin (New Zealand); Sun, Zhifa [Department of Physics, University of Otago, Dunedin (New Zealand); Ali, M. Azam [Department of Applied Sciences, University of Otago, Dunedin (New Zealand)

    2015-11-01

    The properties of chitosan from Arrow squid (Nototodarus sloanii) pen (CHS) and commercial crab shell (CHC) were investigated using FTIR, DSC, SEM and XRD before and after irradiation at the dose of 28 kGy in the presence or absence of 5% water. Also, the viscosity, deacetylation degree, water and oil holding capacities, colour and antimicrobial activities of the chitosan samples were determined. Irradiation decreased (P < 0.05) the viscosity of CHC from 0.21 to 0.03 Pa s and of CHS from 1.71 to 0.23 Pa s. The inclusion of water had no effect on the viscosity of irradiated chitosan. Irradiation did not affect the degree of deacetylation of CHC, but increased the deacetylation degree of CHS from 72.78 to 82.29% in samples with 5% water. Water and oil holding capacities of CHS (1197.30% and 873.3%, respectively) were higher (P < 0.05) than those found in CHC (340.70% and 264.40%, respectively). The water and oil holding capacities were decreased for both types of chitosan irradiation, but were not affected by the addition of water. Squid pen chitosan was whiter in colour (White Index = 90.06%) compared to CHC (White Index = 83.70%). Generally, the CHC samples (control and irradiated) exhibited better antibacterial activity compared to CHS, but the opposite was observed with antifungal activity. - Highlights: • Chitosan prepared from Arrow squid pens (Nototodarus sloanii). • Chitosan samples were gamma irradiated at 28 kGy. • Squid pen chitosan showed high fat and water uptake capacities compared to crab shell chitosan. • Gamma irradiation enhanced the DDA of squid pen chitosan but not crab shell chitosan.

  4. Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites

    Institute of Scientific and Technical Information of China (English)

    Ya-e ZHAO; Zheng-hang WANG; Yang XU; Ji-ru XU; Wen-yan LIU; Meng WEI; Chu-ying WANG

    2012-01-01

    To our knowledge,few reports on Demodex studied at the molecular level are available at present.In this study our group,for the first time,cloned,sequenced and analyzed the chitin synthase (CHS) gene fragments of Demodex folliculorum,Demodex brevis,and Demodex canis (three isolates from each species) from Xi'an China,by designing specific primers based on the only partial sequence of the CHS gene of D.canis from Japan,retrieved from GenBank.Results show that amplification was successful only in three D.canis isolates and one D.brevis isolate out of the nine Demodex isolates.The obtained fragments were sequenced to be 339 bp for D.canis and 338 bp for D.brevis.The CHS gene sequence similarities between the three Xi'an D.canis isolates and one Japanese D.canis isolate ranged from 99.7% to 100.0%,and those between four D.canis isolates and one D.brevis isolate were 99.1%-99.4%.Phylogenetic trees based on maximum parsimony (MP) and maximum likelihood (ML) methods shared the same clusters,according with the traditional classification.Two open reading frames (ORFs) were identified in each CHS gene sequenced,and their corresponding amino acid sequences were located at the catalytic domain.The relatively conserved sequences could be deduced to be a CHS class A gene,which is associated with chitin synthesis in the integument of Demodex mites.

  5. Comparison of dendritic cell-mediated immune responses among canine malignant cells.

    Science.gov (United States)

    Tamura, Kyoichi; Arai, Hiroyoshi; Ueno, Emi; Saito, Chie; Yagihara, Hiroko; Isotani, Mayu; Ono, Kenichiro; Washizu, Tsukimi; Bonkobara, Makoto

    2007-09-01

    Dendritic cell (DC) vaccination is one of the most attractive immunotherapies for malignancies in dogs. To examine the differences in DC-mediated immune responses from different types of malignancies in dogs, we vaccinated dogs using autologous DCs pulsed with keyhole limpet hemocyanin (KLH) and cell lysate prepared from squamous cell carcinoma SCC2/88 (SCC-KLH-DC), histiocytic sarcoma CHS-5 (CHS-KLH-DC), or B cell leukemia GL-1 (GL-KLH-DC) in vitro. In vivo inductions of immune responses against these tumor cells were compared by the delayed-type hypersensitivity (DTH) skin test. The DTH response against SCC2/88 cells were observed in dogs vaccinated with autologous SCC-KLH-DC, while the response was undetectable against CHS-5 and GL-1 cells in dogs vaccinated with autologous CHS-KLH-DC and GL-KLH-DC. Skin biopsies taken from DTH challenge sites were then examined for immunohistochemistry, and recruitment of CD8 and CD4 T cells was detected at the site where SCC2/88 cells were inoculated in dogs vaccinated with SCC-KLH-DC. By contrast, neither CD8 nor CD4 T cell infiltration was found at the DTH challenge site in the dogs vaccinated with CHS-KLH-DC or GL-KLH-DC. These findings may reflect that the efficacy of immune induction by DC vaccination varies among tumor types and that immune responses could be inducible in squamous cell carcinoma. Our results encouraged further investigation of therapeutic vaccination for dogs with advanced squamous cell carcinoma in clinical trials.

  6. High dose dietary pyridoxine induces T-helper type 1 polarization and decreases contact hypersensitivity response to fluorescein isothiocyanate in mice.

    Science.gov (United States)

    Kobayashi, Chie; Kurohane, Kohta; Imai, Yasuyuki

    2012-01-01

    Pyridoxine (vitamin B(6)) is commonly used as a dietary supplement and beneficial effects of it are expected. However, excess ingestion of pyridoxine has been shown to cause a severe sensory neuropathy in humans and experimental animals. We have been studying the linkage between the nervous and immune systems using a fluorescein isothiocyanate (FITC)-induced contact hypersensitivity (CHS) mouse model. We have found that activation of transient receptor potential ankyrin 1 (TRPA1), which is expressed on sensory neurons, enhances skin sensitization to FITC. Another feature of FITC-induced CHS is its dependence on T helper 2 (Th2) type responses. We hypothesized that the excess intake of pyridoxine may affect sensitization to FITC and influence helper T-cell polarization. We examined FITC-induced CHS in BALB/c mice fed a diet containing excess pyridoxine (120 mg/kg diet) for 3 weeks. We found that mice fed on the excess-pyridoxine diet exhibited a lower response as to FITC-induced CHS compared with ones fed on a diet with a standard pyridoxine content (6.0 mg/kg diet). Moreover, the interferon (IFN)-γ/interleukin (IL)-4 ratio produced by draining lymph node cells was significantly higher with the excess-pyridoxine diet. This suggested that the cytokine balance was shifted toward Th1 with the excess-pyridoxine diet. Consistently, Th1-dependent oxazolone-induced CHS was enhanced with the excess-pyridoxine diet. These results suggested that an excess pyridoxine intake actively influences the immune system by altering helper T cell polarization.

  7. Requirement of interaction between mast cells and skin dendritic cells to establish contact hypersensitivity.

    Directory of Open Access Journals (Sweden)

    Atsushi Otsuka

    Full Text Available The role of mast cells (MCs in contact hypersensitivity (CHS remains controversial. This is due in part to the use of the MC-deficient Kit (W/Wv mouse model, since Kit (W/Wv mice congenitally lack other types of cells as a result of a point mutation in c-kit. A recent study indicated that the intronic enhancer (IE for Il4 gene transcription is essential for MCs but not in other cell types. The aim of this study is to re-evaluate the roles of MCs in CHS using mice in which MCs can be conditionally and specifically depleted. Transgenic Mas-TRECK mice in which MCs are depleted conditionally were newly generated using cell-type specific gene regulation by IE. Using this mouse, CHS and FITC-induced cutaneous DC migration were analyzed. Chemotaxis assay and cytoplasmic Ca²⁺ imaging were performed by co-culture of bone marrow-derived MCs (BMMCs and bone marrow-derived dendritic cells (BMDCs. In Mas-TRECK mice, CHS was attenuated when MCs were depleted during the sensitization phase. In addition, both maturation and migration of skin DCs were abrogated by MC depletion. Consistently, BMMCs enhanced maturation and chemotaxis of BMDC in ICAM-1 and TNF-α dependent manners Furthermore, stimulated BMDCs increased intracellular Ca²⁺ of MC upon direct interaction and up-regulated membrane-bound TNF-α on BMMCs. These results suggest that MCs enhance DC functions by interacting with DCs in the skin to establish the sensitization phase of CHS.

  8. Cloning and Expression Analysis of Chilling-induced Chalcone Synthase Gene from Jatropha curcas%小桐子低温诱导查耳酮合酶基因的克隆及其表达分析

    Institute of Scientific and Technical Information of China (English)

    王海波; 邹竹荣; 龚明

    2015-01-01

    In order to understand the function of chalcone synthase in cold resistance of Jatropha curcas, a chilling-induced chalcone synthase gene, named as JcCHS, was cloned from J. curcas based on data of transcriptome and digital gene expression proifling (DGE), and the expression patterns were analyzed by semi-quantitative RT-PCR. The results showed that the full length of JcCHS cDNA was 1386 bp, with entire open reading frame (ORF) of 1170 bp encoding 389 amino acids. The theoretical molecular weight of JcCHS was 42.2 kDa with pI of 6.53. JcCHS protein shared 93.6%identity with CHS from Ricinus communis (Euphorbiaceae), and belong to the type-III polyketide synthase family containing conserved domains of chalcone synthase/styrene synthase. The expression of JcCHS was detected in all tissues with the highest expression in root. In addition, the over-expression of JcCHS in yeast enhanced its resistance to low temperature. So, it was suggested that JcCHS gene would involve in the cold-resistance formation in J. curcas.%为了解查耳酮合酶在小桐子(Jatropha curcas)抗冷性形成中的作用,基于小桐子低温锻炼转录组和数字基因表达谱数据,克隆了低温新诱导表达的小桐子查耳酮合酶基因(JcCHS),并分析了该基因的表达特性和功能。结果表明,JcCHS基因的cDNA全长为1386 bp,包含完整开放阅读框(ORF)1170 bp,编码389个氨基酸,JcCHS的理论分子量为42.2 kDa、等电点为6.53,与蓖麻CHS蛋白序列的相似性高达93.6%,具有III型聚酮合酶家族保守的查耳酮合酶/对苯乙烯合酶结构域。半定量RT-PCR分析表明,JcCHS在小桐子各组织中都有表达,其中根的表达量较高。JcCHS基因的表达能在一定程度上提高重组酵母菌的低温抵抗能力,这说明JcCHS基因可能参与了小桐子的抗低温响应。

  9. Gene specificity of suppression of transgene-mediated insertional transcriptional activation by the chicken HS4 insulator.

    Directory of Open Access Journals (Sweden)

    Romain Desprat

    Full Text Available Insertional mutagenesis has emerged as a major obstacle for gene therapy based on vectors that integrate randomly in the genome. Reducing the genotoxicity of genomic viral integration can, in first approximation, be equated with reducing the risk of oncogene activation, at least in the case of therapeutic payloads that have no known oncogenic potential, such as the globin genes. An attractive solution to the problem of oncogene activation is the inclusion of insulators/enhancer-blockers in the viral vectors. In this study we have used Recombinase-Mediated Cassette Exchange to characterize the effect of integration of globin therapeutic cassettes in the presence or absence of the chicken HS4 and three other putative insulators inserted near Stil, Tal1 and MAP17, three well-known cellular proto-oncogenes in the SCL/Tal1 locus. We show that insertion of a Locus Control Region-driven globin therapeutic globin transgene had a dramatic activating effect on Tal1 and Map17, the two closest genes, a minor effect on Stil, and no effect on Cyp4x1, a non-expressed gene. Of the four element tested, cHS4 was the only one that was able to suppress this transgene-mediated insertional transcriptional activation. cHS4 had a strong suppressive effect on the activation expression of Map17 but has little or no effect on expression of Tal1. The suppressive activity of cHS4 is therefore promoter specific. Importantly, the observed suppressive effect of cHS4 on Map17 activation did not depend on its intercalation between the LCR and the Map 17 promoter. Rather, presence of one or two copies of cHS4 anywhere within the transgene was sufficient to almost completely block the activation of Map17. Therefore, at this complex locus, suppression of transgene-mediated insertional transcriptional activation by cHS4 could not be adequately explained by models that predict that cHS4 can only suppress expression through an enhancer-blocking activity that requires intercalation

  10. Immuno-protection of SJIR-2 DNA vaccine with microspheres adjuvant in mice challenged with Schistosoma japonicum%日本血吸虫SJIR-2纳米微球核酸疫苗的免疫保护性研究

    Institute of Scientific and Technical Information of China (English)

    王正印; 潘丽红; 汪学龙

    2016-01-01

    目的:研究日本血吸虫胰岛素受体-2( SJIR-2)纳米微球核酸疫苗对小鼠攻击感染的免疫保护效果。方法构建pEGFP-SJIR-2重组质粒,双酶切鉴定并测序,大量提取pEGFP-SJIR-2质粒,用壳聚糖( CHS)修饰的聚乳酸-羟基乙酸共聚物( PLGA)微球包裹,用包裹后的SJIR-2纳米微球免疫小鼠。将40只雌性BALB/c小鼠随机分为4组( n=10),分别注射 PBS、空 pEGFP 质粒、CHS-PLGA 微球和 CHS-PL-GA-pEGFP-SJIR-2微球各100μg免疫小鼠,末次免疫2周后,用日本血吸虫尾蚴攻击感染小鼠,每次免疫及感染尾蚴前收集各组小鼠血清,ELISA法检测各组小鼠血清内免疫球蛋白( IgG)水平的变化。小鼠感染尾蚴42 d后全部剖杀,收集成虫和虫卵并计算减虫率和减卵率。结果成功构建了pEGFP-SJIR-2重组质粒,与PBS组比较, CHS-PLGA-pEGFP-SJIR-2组的成虫数和虫卵数差异有统计学意义( P<0.01)。CHS-PLGA-pEGFP-SJIR-2组的减虫率和减卵率分别为37.36%和46.82%,和PBS组相比,CHS-PLGA-pEGFP-SJIR-2组小鼠血清内IgG水平比明显增高( P<0.01),而pEGFP组和CHS-PLGA组成虫数和虫卵数与PBS组比较差异无统计学意义。结论 SJIR-2纳米微球核酸疫苗对感染血吸虫的BALB/c小鼠有一定的免疫保护效果,对其潜在的候选抗原疫苗的价值尚需深入研究。%Objective To research the immuno-protection of SJIR-2 DNA vaccine with nanometer microspheres a-gainst Schistosoma japonicum infection in mice. Methods To construct eukaryotic expression plasmid pEGFP-SJIR-2, identified by double digestion and sequenced delivery. The recombinant plasmid pEGFP-SJIR-2 was ex-tracted and was encapsulated into PLGA nanometer microspheres which were modified by CHS. 40 female BALB/c mice were randomly divided into 4 groups (n=10), each group of mice were injected with PBS, empty pEGFP plasmid, CHS-PLGA nanometer microspheres and CHS-PLGA-pEGFP-SJIR-2 nanometer microspheres 100 μg, re-spectively. Two weeks

  11. Chediak-Higashi syndrome and premature exfoliation of primary teeth.

    Science.gov (United States)

    Rezende, Karla Mayra; Canela, Alfredo Hiram Carrillo; Ortega, Adriana Oliveira Lira; Tintel, Claudia; Bönecker, Marcelo

    2013-01-01

    The Chediak-Higashi syndrome (CHS) is a rare hereditary fatal disease, if not treated. These changes are associated with various diseases and syndromes that mainly cause periodontal disease and thus the premature loss of teeth. This paper describes the monitoring of premature loss of primary teeth that began when the child was 5 years old. On presentation his teeth were mobile and there was a history of gingival bleeding. Panoramic radiography revealed generalized and severe bone loss, and the teeth showed no bony support enough for their stability. Blood test was ordered to assess the overall health of the child and giant cells with cytoplasmic granules were found, confirming the diagnosis of CHS. The management of periodontal disease focused on the control of infection and bacterial plaque by mechanical and chemical methods.

  12. Rich: Region-based Intelligent Cluster-Head Selection and Node Deployment Strategy in Concentric-based WSNs

    Directory of Open Access Journals (Sweden)

    FAN, C.-S.

    2013-11-01

    Full Text Available In a random deployment, sensor nodes are scattered randomly in the sensing field. Hence, the coverage can not be guaranteed. In contrast, the coverage of uniformly deployment is in general larger than the random deployment. However, uniformly deployment strategy may cause unbalanced traffic pattern in wireless sensor networks (WSNs. In this situation, larger load may be imposed to CHs (cluster heads around the sink. Therefore, CHs close to the sink use up their energy earlier than those farther away from the sink. To overcome this problem, we propose a novel node deployment strategy in the concentric model, namely, Region-based Intelligent Cluster-Head selection and node deployment strategy (called Rich. The coverage, energy consumption and data routing issues are well investigated and taken into consideration in the proposed Rich scheme. The simulation results show that the proposed Rich alleviates the unbalanced traffic pattern significantly, prolongs network lifetime and achieves satisfactory coverage ratio.

  13. Syndromic albinism: a review of genetics and phenotypes.

    Science.gov (United States)

    Scheinfeld, Noah S

    2003-12-01

    There are several syndromes of albinism associated with systemic pathology. These include Chediak-Higashi Syndrome (CHS), Hermansky-Pudlack Syndrome (HPS), Griscelli Syndrome (GS), Elejalde Syndrome (ES) and Cross-McKusick-Breen Syndrome (CMBS). In the last several years the genetic defects underlying some of these syndromes have been described. HPS is related to 7 genes in humans. GS is related to 3 genes: MYOVA, Rab-27A, and melanophilin (Mlph). CHS is related to one gene: LYST. The genetic defects in ES and CMBS are yet to be defined. Syndromic forms of albinism are associated with defects in the packaging of melanin and other cellular proteins. As such they are distinct from oculocutaneous albinism, which is associated with defects in the production of melanin (e.g., TRP1, P gene, and tyrosinase).

  14. Fluticasone as Treatment for Cluster Headaches in a Navy Aircrew Member.

    Science.gov (United States)

    Andicochea, Chad T; Blalack, Penelope M; Fulkerson, Joel A; Kidd, Grant A; Taylor, Bradley M

    2015-10-01

    Cluster headaches (CHs) are one of the most debilitating of all the identified headache syndromes. Their pathogenesis is poorly understood, which has made their treatment challenging. Various medicines and modalities have been put forth in an effort to minimize the symptoms, but none have proven to be consistently reliable. For the active duty military aviator with CHs, this is further compounded by the administrative restrictions to use many of those medications secondary to their unwanted and potentially dangerous side effects. We present a case of refractory CH in an active duty air crewman, treated with fluticasone, which resulted in a remarkable reduction in symptoms allowing him to resume his primary flight duties. We propose that fluticasone's effectiveness in improving his CH symptoms stems from its anti-inflammatory properties.

  15. In silicio expression analysis of PKS genes isolated from Cannabis sativa L.

    Science.gov (United States)

    Flores-Sanchez, Isvett J; Linthorst, Huub J M; Verpoorte, Robert

    2010-10-01

    Cannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs) are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids and stilbenoids. Biosynthetically, the cannabinoids are polyketide substituted with terpenoid moiety. Using an RT-PCR homology search, PKS cDNAs were isolated from cannabis plants. The deduced amino acid sequences showed 51%-73% identity to other CHS/STS type sequences of the PKS family. Further, phylogenetic analysis revealed that these PKS cDNAs grouped with other non-chalcone-producing PKSs. Homology modeling analysis of these cannabis PKSs predicts a 3D overall fold, similar to alfalfa CHS2, with small steric differences on the residues that shape the active site of the cannabis PKSs.

  16. Antioxidant enzymes as potential targets in cardioprotection and treatment of cardiovascular diseases. Enzyme antioxidants: the next stage of pharmacological counterwork to the oxidative stress

    Directory of Open Access Journals (Sweden)

    Alexander V. Vavaev

    2012-02-01

    Full Text Available The focus in antioxidant research is on enzyme derivative investigations. Extracellular superoxide dismutase (EC-SOD is of particular interest, as it demonstrates in vivo the protective action against development of atherosclerosis, hypertension, heart failure, diabetes mellitus. The reliable association of coronary artery disease with decreased level of heparin-released EC-SOD was established in clinical research. To create a base for and to develop antioxidant therapy, various SOD isozymes, catalase (CAT, methods of gene therapy, and combined applications of enzymes are used. Covalent bienzyme SOD-CHS-CAT conjugate (CHS, chondroitin sulphate showed high efficacy and safety as the drug candidate. There is an evident trend to use the components of glycocalyx and extracellular matrix for target delivery of medical substances. Development of new enzyme antioxidants for therapeutic application is closely connected with progress in medical biotechnology, pharmaceutical industry, and bioeconomy.

  17. In silicio expression analysis of PKS genes isolated from Cannabis sativa L.

    Directory of Open Access Journals (Sweden)

    Isvett J. Flores-Sanchez

    2010-01-01

    Full Text Available Cannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids and stilbenoids. Biosynthetically, the cannabinoids are polyketide substituted with terpenoid moiety. Using an RT-PCR homology search, PKS cDNAs were isolated from cannabis plants. The deduced amino acid sequences showed 51%-73% identity to other CHS/STS type sequences of the PKS family. Further, phylogenetic analysis revealed that these PKS cDNAs grouped with other non-chalcone-producing PKSs. Homology modeling analysis of these cannabis PKSs predicts a 3D overall fold, similar to alfalfa CHS2, with small steric differences on the residues that shape the active site of the cannabis PKSs.

  18. Cytokines and Langerhans cells in allergic contact dermatitis.

    Science.gov (United States)

    Elsaie, M L; Olasz, E; Jacob, S E

    2008-06-01

    Contact hypersensitivity (CHS) is a dendritic cell (DC)-dependent T-cell mediated cutaneous inflammatory reaction elicited by epicutaneous exposure to reactive chemicals, known as haptens, from cosmetic products or through environmental and occupational exposures. The best-studied haptens are low molecular weight chemicals (contact dermatitis. Haptens penetrate the skin and bind to self proteins to form complete antigens which are taken by antigen presenting cells to start a cascade of actions resulting in a delayed hypersensitivity reaction. Larger molecules such as proteins induce response involving the humoral immune system. The environment at the time of antigen presentation affects the innate immune system which in turn influences the expression of CHS. The subsequent immunologic response (or lack thereof) is a result of complex interaction between both the innate and the adaptive immune systems. This interaction results in either an inflammatory immune response or tolerance.

  19. 漫湾水电厂转轮上冠过流面汽蚀的处理%Runner crown flow surface cavitation treatment of Manwan Power Plant

    Institute of Scientific and Technical Information of China (English)

    季兴光; 岳建锋; 曲岩

    2012-01-01

      介绍了漫湾水电站的工程概况及水轮发电机转轮上冠过流面存在的汽蚀问题,分析了转轮上冠过流面的材料性能,论述了利用CHS237不锈钢焊条焊接钢板的工艺流程及方法。%  An introduction was made on the general of Manwan Power Plant and cavitation damage existing on the runner crown flow surface, based on which the authors analyze the properties of flow surface material, present the technical process and methods of welding steel plates by use of CHS237 stainless rods.

  20. [Protective action figurations for superoxide dismutase - chondroitin sulfate - catalase bienzyme conjugate after its medicative administration in endotoxin shock].

    Science.gov (United States)

    Maksimenko, A V; Vavaeva, A V; Zvyagintseva, M A; Abramov, A A; Timoshin, A A; Vavaev, A V; Lakomkin, V L

    2016-03-01

    Previously it found that the bienzymatic conjugate superoxide dismutase-chondroitin sulfate, catalase (SOD-CHS-CAT) increased the survival rate of rats with endotoxic shock caused by the administration of lipopolysaccharide (LPS). This effect was observed both in preventive (before LPS) and therapeutic conjugate administration (after the administration of LPS). This study shows that the development of endotoxic shock is accompanied by increased levels of NO in the liver, lungs, kidneys, heart; administration of the SOD-CHS-CAT conjugate insignificantly influenced this parameter. At the same time, the changes in blood urea and creatinine suggest the protective effect of the conjugate on renal function, while diverse changes in biochemical parameters studied complicate the formation of the agreed conclusions on the state of other organs.

  1. Protocol for the application of cooperative MIMO based on clustering in sparse wireless sensor networks

    Institute of Scientific and Technical Information of China (English)

    WANG Qing-hua; QU Yu-gui; LIN Zhi-ting; BAI Rong-gang; ZHAO Bao-hua; PAN Quan-ke

    2007-01-01

    Wireless sensor networks (WSN) using cooperative multiple-input multiple-output (MIMO) communication are effective tools to collect data in several environments. However, how to apply cooperative MIMO in WSN remains a critical challenge, especially in sparse WSN. In this article, a novel clustering scheme is proposed for the application of cooperative MIMO in sparse WSN by extending the traditional low-energy adaptive clustering hierarchy (LEACH) protocol. This clustering scheme solves the problem that the cluster heads (CH) cannot find enough secondary cluster heads (SCH), which are used to cooperate and inform multiple-antenna transmitters with CHs. On the basis of this protocol, the overall energy consumption of the networks model is developed, and the optimal number of CHs is obtained. The simulation results show that this protocol is feasible for the sparse WSN. The simulation results also illustrate that this protocol provides significant energy efficiencies, even after allowing for additional overheads.

  2. Antibiotic-resistant organisms cultured from Atlantic bottlenose dolphins (Tursiops truncatus) inhabiting estuarine waters of Charleston, SC and Indian River Lagoon, FL.

    Science.gov (United States)

    Schaefer, Adam M; Goldstein, Juli D; Reif, John S; Fair, Patricia A; Bossart, Gregory D

    2009-03-01

    Bottlenose dolphins (Tursiops truncatus) from estuarine waters of Indian River Lagoon, FL (IRL) and Charleston, SC (CHS) were cultured to screen for microorganism colonization and to assess antibiotic sensitivity. Swabs (n = 909) were collected from the blowhole, gastric fluid, and feces of 171 individual dolphins The most frequently cultured organisms were Plesiomonas shigelloides (n = 161), Aeromonas hydrophila (n = 144), Escherichia coli (n = 85), and Pseudomonas fluorescens (n = 82). In descending frequency, organisms demonstrated resistance to erythromycin, ampicillin, and cephalothin. Human and animal pathogens resistant to antibiotics used in human and veterinary medicine were cultured. Escherichia coli (E. coli) more often was resistant in IRL dolphins. Three cases of methicillin-resistant Staphylococcus aureus (MRSA) were found at CHS. Emergence of antibiotic resistance is not confined to humans. Bottlenose dolphins may serve as sentinels for transfer of resistance from humans and animals or indicate that antibiotics are reaching the marine environment and causing resistance to emerge through selective pressure and genetic adaptation.

  3. Morbillivirus infection in free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) from the Southeastern United States: seroepidemiologic and pathologic evidence of subclinical infection.

    Science.gov (United States)

    Bossart, Gregory D; Reif, John S; Schaefer, Adam M; Goldstein, Juli; Fair, Patricia A; Saliki, Jeremiah T

    2010-07-14

    From 2003 to 2007, sera (n=234) from free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) inhabiting two southeast Atlantic estuarine regions, the Indian River Lagoon (IRL), FL and Charleston, SC (CHS) were tested for antibodies to cetacean morbilliviruses as part of a multidisciplinary study of individual and population health. Positive morbillivirus titers were found on initial capture in 12 of 122 (9.8%) IRL dolphins in the absence of an epizootic. All CHS dolphins were seronegative. Positive fluctuating morbillivirus titers and seroconversion were found in IRL dolphins. Seropositivity was detected in dolphins 8-13 years of age as well as in dolphins that were alive during the 1987-1988 epizootic. During the study period, pathologic and immunohistochemical findings from stranded IRL dolphins (n=14) did not demonstrate typical morbillivirus-associated lesions or the presence of morbillivirus antigen. The findings suggest that morbillivirus infections are occurring in the absence of widespread mortality in IRL dolphins.

  4. Morphology engineering of Penicillium chrysogenum by RNA silencing of chitin synthase gene.

    Science.gov (United States)

    Liu, Hui; Wang, Peng; Gong, Guohong; Wang, Li; Zhao, Genhai; Zheng, Zhiming

    2013-03-01

    Chitin synthases, that catalyze the formation of chitin the major component of cell walls in most filamentous fungi, play crucial roles in the growth and morphogenesis. To investigate the roles of chitin synthase in Penicillium chrysogenum, we developed an RNAi system to silence the class III chitin synthase gene chs4. After transformation, mutants had a slow growth rate and shorter but highly branched hyphae. All transformants either were unable to form conidia or could form only a few. Changes in chs4 expression could lead to a completely different morphology and eventually cause distinct penicillin yields. In particular, the yield of one transformant was 41 % higher than that of the original strain.

  5. Hydrogen-bonding interactions between [BMIM][BF4] and dimethyl sulfoxide

    Science.gov (United States)

    Zheng, Yan-Zhen; He, Hong-Yan; Zhou, Yu; Yu, Zhi-Wu

    2014-07-01

    Mixtures of Ionic liquids and small polar organic solvent are potential green solvents for cellulose dissolution under mild conditions. In this work, the interactions between a representative imidazolium-based ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]) and dimethyl sulfoxide (DMSO) were investigated in detail by attenuated total reflection infrared spectroscopy (ATR-IR) and density functional theory calculations (DFT). The main conclusions are: (1) C2-H is the main interaction site in forming cation-anion, cation-DMSO, and [BMIM][BF4]-DMSO complexes. (2) The two turning points of the wavenumber shift changes of C2-H may indicate that the dilution process can be divided into several stages: from larger ion clusters to smaller ion clusters, then to ion pairs, and finally to individual ions. The solvent molecules cannot break apart the strong Coulombic interaction between [BMIM]+ and [BF4]- but can break apart the ion clusters into ion pairs when the mole fraction of DMSO is less than 0.9. When the mole fraction of DMSO is greater than 0.9, ion pairs can be broke into ions. (3) The hydrogen-bonds of the aromatic C-Hs in [BMIM]+ are strengthened in the dilution process while those of the alkyl C-Hs of [BMIM]+ are weakened. (4) The aromatic C-Hs of the [BMIM]+ cation strength before the weakening of the alkyl C-Hs. These in-depth studies on the properties of the ionic liquid-DMSO mixed solvents may shed light on exploring their applications as mixed solvents in cellulose dissolution and other practices.

  6. Benzophenone Synthase and Chalcone Synthase Accumulate in the Mesophyll of Hypericum perforatum Leaves at Different Developmental Stages

    OpenAIRE

    Belkheir, Asma K.; Gaid, Mariam; Liu, Benye; Hänsch, Robert; Beerhues, Ludger

    2016-01-01

    The active medicinal constituents in Hypericum perforatum, used to treat depression and skin irritation, include flavonoids and xanthones. The carbon skeletons of these compounds are formed by chalcone synthase (CHS) and benzophenone synthase (BPS), respectively. Polyclonal antisera were raised against the polyketide synthases from Hypericum androsaemum and their IgG fractions were isolated. Immunoblotting and immunotitration were used to test the IgGs for crossreactivity and monospecificity ...

  7. Analysis of community health service team in service mode%社区卫生服务团队服务模式的调查分析

    Institute of Scientific and Technical Information of China (English)

    董燕敏; 尹德卢; 李媛媛; 罗洁; 王媛

    2013-01-01

    选取在全国较早开展“全科团队服务”的五城市29个社区卫生服务(CHS)中心与526名团队成员进行问卷调查.共组建团队275个,团队成员占在岗职工40.8%.辖区居委会覆盖率达100%.团队组合模式分为:医护防经典组合型、大团队专业扩充型、小团队医护组合型与非卫技广泛参与型4种.团队运行各有特色,但仍存在一些问题,建议进一步加强社区卫生服务队伍建设,完善团队合作服务模式,营造CHS发展良好氛围并加大社会宣传力度.%A total of 526 team members from 28 community health service (CHS) centers in 5 pilot cities were surveyed by a questionnaire.All CHS centers developed team service.And 275 service teams were established and the coverage rate of area resident committee was 100%.The teaming modes were mainly divided into four kinds:medical-nursing-prevention classic combination,big team professional expansion,small team medical combination and non-health technology extensive participation.The team services showed multiple advantages of professionism,energy and time complementing.We should strengthen team construction,improve perfect team service mode and policy support and create a good environment for CHS development.

  8. Extraosseous, Epidural Cavernous Hemangioma with Back Pain

    Science.gov (United States)

    Ozkal, Birol; Yaldiz, Can; Yaman, Onur; Ozdemır, Nail; Dalbayrak, Sedat

    2015-01-01

    Summary Background Cavernous malformations are characterized by enlarged vascular structures located in benign neural tissues within the cerebellum and spinal cord of the central nervous system. Cavernous hemangiomas (CHs) account for 5% to 12% of all spinal vascular malformations. Case Report We removed a hemorrhagic thoracic mass in a 40-year-old male patient who presented with progressive neurological deficits. Conclusions We found it appropriate to present this case due to its rarity. PMID:25960818

  9. Transgene-induced gene silencing is not affected by a change in ploidy level.

    Directory of Open Access Journals (Sweden)

    Daniela Pignatta

    Full Text Available BACKGROUND: Whole genome duplication, which results in polyploidy, is a common feature of plant populations and a recurring event in the evolution of flowering plants. Polyploidy can result in changes to gene expression and epigenetic instability. Several epigenetic phenomena, occurring at the transcriptional or post-transcriptional level, have been documented in allopolyploids (polyploids derived from species hybrids of Arabidopsis thaliana, yet findings in autopolyploids (polyploids derived from the duplication of the genome of a single species are limited. Here, we tested the hypothesis that an increase in ploidy enhances transgene-induced post-transcriptional gene silencing using autopolyploids of A. thaliana. METHODOLOGY/PRINCIPAL FINDINGS: Diploid and tetraploid individuals of four independent homozygous transgenic lines of A. thaliana transformed with chalcone synthase (CHS inverted repeat (hairpin constructs were generated. For each line diploids and tetraploids were compared for efficiency in post-transcriptional silencing of the endogenous CHS gene. The four lines differed substantially in their silencing efficiency. Yet, diploid and tetraploid plants derived from these plants and containing therefore identical transgene insertions showed no difference in the efficiency silencing CHS as assayed by visual scoring, anthocyanin assays and quantification of CHS mRNA. CONCLUSIONS/SIGNIFICANCE: Our results in A. thaliana indicated that there is no effect of ploidy level on transgene-induced post-transcriptional gene silencing. Our findings that post-transcriptional mechanisms were equally effective in diploids and tetraploids supports the use of transgene-driven post-transcriptional gene silencing as a useful mechanism to modify gene expression in polyploid species.

  10. Disparities in depressive distress by sexual orientation in emerging adults: the roles of attachment and stress paradigms.

    Science.gov (United States)

    Rosario, Margaret; Reisner, Sari L; Corliss, Heather L; Wypij, David; Frazier, A Lindsay; Austin, S Bryn

    2014-07-01

    Lesbian, gay, and bisexual (BI) youth have elevated rates of depression compared to heterosexuals. We proposed and examined a theoretical model to understand whether attachment and stress paradigms explain disparities in depressive distress by sexual orientation, using the longitudinal Growing Up Today Study (GUTS) and Nurses' Health Study II (NHSII). GUTS participants eligible for this analysis reported sexual orientation, childhood gender nonconforming behaviors (GNBs), attachment to mother (all in 2005), and depressive symptoms (in 2007). Mothers of the GUTS participants who are the NHSII participants reported attitudes toward homosexuality (in 2004) and maternal affection (in 2006). The sample had 6,122 participants. Of GUTS youth (M = 20.6 years old in 2005; 64.4 % female), 1.7 % were lesbian/gay (LG), 1.7 % bisexual (BI), 10.0 % mostly heterosexual (MH), and 86.7 % completely heterosexual (CH). After adjusting for demographic characteristics and sibling clustering, LGs, BIs, and MHs reported more depressive distress than CHs. This relation was partially mediated (i.e., explained) for LGs, BIs, and MHs relative to CHs by less secure attachment. A conditional relation (i.e., interaction) indicated that BIs reported more distress than CHs as GNBs increased for BIs; no comparable relation was found for LGs versus CHs. Sibling comparisons found that sexual minorities (LGs, BIs, and MHs) reported more depressive distress, less secure attachment, and more childhood GNBs than CH siblings; the mothers reported less affection for their sexual-minority than CH offspring. The findings suggest that attachment and childhood gender nonconformity differentially pattern depressive distress by sexual orientation. Attachment and related experiences are more problematic for sexual minorities than for their CH siblings.

  11. Dairy food intake is positively associated with cardiovascular health: findings from Observation of Cardiovascular Risk Factors in Luxembourg study.

    Science.gov (United States)

    Crichton, Georgina E; Alkerwi, Ala'a

    2014-12-01

    Conflicting findings have been reported about dairy food consumption and risk for cardiovascular disease. Furthermore, few studies have examined dairy food intake in relation to cardiovascular health and the incorporation of lifestyle factors such as diet and physical activity. This study examined whether dairy food consumption was associated with cardiovascular health, recently defined by the American Heart Association. Data were analyzed from 1352 participants from the Observation of Cardiovascular Risk Factors in Luxembourg survey. A validated food frequency questionnaire was used to measure intakes of milk, yogurt, cheese, dairy desserts, ice cream, and butter. Seven cardiovascular health metrics were assessed: smoking, body mass index, physical activity, diet, total cholesterol, blood pressure, and fasting plasma glucose. A total cardiovascular health score (CHS) was determined by summing the total number of health metrics at ideal levels. It was hypothesized that greater dairy food consumption (both low fat and whole fat) would be associated with better global cardiovascular health, as indicated by a higher CHS. Total dairy food intake was positively associated with the CHS. Higher intakes of whole fat milk, yogurt, and cheese were associated with better cardiovascular health. Even when controlling for demographic and dietary variables, those who consumed at least 5 servings per week of these dairy products had a significantly higher CHS than those who consumed these products less frequently. Higher total whole fat dairy food intake was also associated with other positive health behaviors, including being a nonsmoker, consuming the suggested dietary intakes of recommended foods, and having a normal body mass index. Increased dairy food consumption was associated with better cardiovascular health.

  12. Human mitochondrial HMG CoA synthase: Liver cDNA and partial genomic cloning, chromosome mapping to 1p12-p13, and possible role in vertebrate evolution

    Energy Technology Data Exchange (ETDEWEB)

    Boukaftane, Y.; Robert, M.F.; Mitchell, G.A. [Hopital Sainte-Justine, Montreal (Canada)] [and others

    1994-10-01

    Mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase (mHS) is the first enzyme of ketogenesis, whereas the cytoplasmic HS isozyme (cHS) mediates an early step in cholersterol synthesis. We here report the sequence of human and mouse liver mHS cDNAs, the sequence of an HS-like cDNA from Caenorhabditis elegans, the structure of a partial human mHS genomic clone, and the mapping of the human mHS gene to chromosome 1p12-p13. the nucleotide sequence of the human mHS cDNA encodes a mature mHS peptide of 471 residues, with a mean amino acid identity of 66.5% with cHS from mammals and chicken. Comparative analysis of all known mHS and cHS protein and DNA sequences shows a high degree of conservation near the N-terminus that decreases progressively toward the C-terminus and suggests that the two isozymes arose from a common ancestor gene 400-900 million years ago. Comparison of the gene structure of mHS and cHS is also consistant with a recent duplication event. We hypothesize that the physiologic result of the HS gene duplication was the appearance of HS within the mitochondria around the time of emergence of early vertebrates, which linked preexisting pathways of beta oxidation and leucine catabolism and created the HMG CoA pathway of ketogenesis, thus providing a lipid-derived energy source for the vertebrate brain. 56 refs., 4 figs., 2 tabs.

  13. Cytochrome P4501A1 expression, polychlorinated biphenyls and hydroxylated metabolites, and adipocyte size of bottlenose dolphins from the Southeast United States.

    Science.gov (United States)

    Montie, Eric W; Fair, Patricia A; Bossart, Gregory D; Mitchum, Greg B; Houde, Magali; Muir, Derek C G; Letcher, Robert J; McFee, Wayne E; Starczak, Victoria R; Stegeman, John J; Hahn, Mark E

    2008-02-18

    Persistent organic pollutants (POPs) bioaccumulate in blubber of marine mammals. Therefore, it is important to understand the structure and dynamics of blubber layers and how they affect the accumulation of POPs and subsequent biochemical responses. We used established histological and immunohistochemical methods to document the structure of bottlenose dolphin (Tursiops truncatus) blubber and to assess the expression of cytochrome P4501A1 (CYP1A1) in skin-blubber biopsies of dolphins sampled in the waters off Charleston, SC (CHS) (N=38), and Indian River Lagoon, FL (IRL) (N=36). CYP1A1 expression was strongest and most frequent in capillary endothelial cells and was stratified in blubber; the greatest CYP1A1 staining was in the deepest layer. CYP1A1 expression in deep blubber and 2,3,7,8-TCDD Toxic Equivalents measured in the entire blubber were significantly higher in dolphins from CHS as compared to those from IRL. Adipocyte size was associated with the extent of CYP1A1 expression. Male dolphins with smaller adipocytes from CHS and IRL had higher levels of CYP1A1 expression in deep blubber. In CHS females, CYP1A1 expression in vascular endothelial cells varied with reproductive status. CYP1A1 expression in the deep layer was highest in simultaneously pregnant-lactating dolphins, and these dolphins had the smallest adipocytes in deep blubber. In all dolphins, CYP1A1 expression in the deep blubber layer was positively related to concentrations of hydroxylated PCBs (OH-PCBs) in plasma. In summary, redistribution of AHR agonists from blubber into the circulatory system may enhance PCB metabolism and production of OH-PCBs by induction of CYP1A1 in hepatocytes and, possibly, by induction of CYP1A1 in endothelial cells of the deep blubber. The OH-PCBs thus formed have the potential to interfere with thyroid hormone homeostasis.

  14. Influences of biological variables and geographic location on circulating concentrations of thyroid hormones in wild bottlenose dolphins (Tursiops truncatus).

    Science.gov (United States)

    Fair, Patricia A; Montie, Eric; Balthis, Len; Reif, John S; Bossart, Gregory D

    2011-11-01

    Thyroid hormones (TH) are key regulators of metabolism and development, yet our understanding of the variability in serum TH concentrations in free-ranging marine mammals is limited. Thus, we examined the interrelationships between TH and age, sex, reproductive status, geographic location, and ocean temperatures in wild bottlenose dolphins (Tursiops truncatus). Circulating concentrations of TH (total thyroxine (tT(4)), free T(4) (fT(4)), and total triiodothyronine (tT(3))) were determined in a total of 195 dolphins; 80 from the coastal waters of Charleston, South Carolina (CHS) and 115 from the Indian River Lagoon, Florida (IRL). Age had the most influence on circulating TH concentrations in dolphins at both sites with decreasing concentrations (p<0.0001) observed with increasing age for all TH. No significant differences were found between males and non-reproductive females. Geographic location significantly influenced tT(4) and tT(3) concentrations; CHS dolphins had higher concentrations than IRL animals. These TH differences between CHS and IRL dolphins may be attributed to the colder year-round water temperature that CHS dolphins inhabit compared to IRL dolphins and could constitute an adaptive response to their colder environment. Results from this study highlight the importance of establishing reference values for dolphins in different geographic locations to support valid comparisons. This initial assessment provides a foundation of how biological and environmental variables could affect circulating TH in dolphins, which will help to elucidate the impacts of disease, pollution, and climate change on the thyroid hormone system of aquatic mammals.

  15. Gender Differences in Tea, Coffee, and Cognitive Decline in the Elderly: The Cardiovascular Health Study

    OpenAIRE

    Arab, Lenore; Biggs, Mary L.; O’Meara, Ellen S.; Longstreth, W. T.; Crane, Paul K.; Fitzpatrick, Annette L.

    2011-01-01

    Although caffeine can enhance cognitive function acutely, long-term effects of consumption of caffeine-containing beverages such as tea and coffee are uncertain. Data on 4,809 participants aged 65 and older from the Cardiovascular Health Study (CHS) were used to examine the relationship of consumption of tea and coffee, assessed by food frequency questionnaire, on change in cognitive function by gender. Cognitive performance was assessed using serial Modified Mini-Mental State (3MS) examinati...

  16. Theoretical spectroscopic characterization at low temperatures of S-methyl thioformate and O-methyl thioformate

    Energy Technology Data Exchange (ETDEWEB)

    Senent, M. L., E-mail: senent@iem.cfmac.csic.es [Departamento de Química y Física Teóricas, Instituto de Estructura de la Materia, IEM-C.S.I.C., Serrano 121, Madrid 28006 (Spain); Puzzarini, C., E-mail: cristina.puzzarini@unibo.it [Dipartimento di Chimica G. Ciamician, Università di Bologna, Via F. Selmi 2, I-40126 Bologna (Italy); Hochlaf, M., E-mail: hochlaf@univ-mlv.fr [Laboratoire de Modélisation et Simulation Multi Echelle, Université Paris-Est, MSME UMR 8208 CNRS, 5 boulevard Descartes, 77454 Marne-la-Vallée (France); Domínguez-Gómez, R., E-mail: rosa.dominguez@upm.es [Departamento de Ingeniería Civil, Cátedra de Química, E.U.I.T. Obras Públicas, Universidad Politécnica de Madrid, Madrid (Spain); Carvajal, M., E-mail: miguel.carvajal@dfa.uhu.es [Departamento de Física Aplicada, Facultad de Ciencias Experimentales, Unidad Asociada IEM-CSIC-U.Huelva, Universidad de Huelva, 21071 Huelva (Spain)

    2014-09-14

    Highly correlated ab initio methods are employed to determine spectroscopic properties at low temperatures of two S-analogs of methyl formate: S-methyl thioformate CH{sub 3}-S-CHO (MSCHO) and O-methyl thioformate CH{sub 3}-O-CHS (MOCHS). Both species are detectable and they are expected to play an important role in Astrochemistry. Molecular properties are compared with those of the O-analog, methyl formate. Both isomers present two conformers cis and trans. cis-CH{sub 3}-S-CHO represents the most stable structure lying 4372.2 cm{sup −1} below cis-CH{sub 3}-O-CHS. The energy difference between the cis and trans forms is drastically lower for MSCHO (1134 cm{sup −1}) than for MOCHS (1963.6 cm{sup −1}). Harmonic and anharmonic fundamentals and the corresponding intensities, as well as the rotational constants for the ground vibrational and first excited torsional states and the centrifugal distortions constants, are provided. Low torsional energy levels have been obtained by solving variationally a two dimensional Hamiltonian expressed in terms of the two torsional degrees of freedom. The corresponding 2D potential energy surfaces have been computed at the CCSD(T)/aug-cc-pVTZ level of theory. The methyl torsional barriers V{sub 3}(cis) are determined to be 139.7 cm{sup −1} (CH{sub 3}-S-CHO) and 670.4 cm{sup −1} (CH{sub 3}-O-CHS). The A/E splitting of ground torsional state has been estimated to be 0.438 cm{sup −1} for CH{sub 3}-S-CHO and negligible for CH{sub 3}-O-CHS.

  17. Development of CAPS markers to identify Indian tea (Camellia sinensis) clones with high catechin content.

    Science.gov (United States)

    Elangbam, M; Misra, A K

    2016-06-10

    Tea leaves are rich in plant secondary phenolics, especially flavonoids. Catechins are considered to be the most valuable flavonoids, and the catechin content in tea is an important trait for determining its quality. We have developed cleaved amplified polymorphic sequence (CAPS)-based markers for evaluating total catechin content that target two important secondary metabolite pathway genes, PAL (phenylalanine ammonia-lyase) and CHS (chalcone synthase). Catechin content levels in the tea samples tested ranged from 9 to 33 mg/mg. The CAPS technique identified clones with the homozygous profile PRc1, which has relatively lower catechin content than clones with the heterozygous profile PRc2. A significant difference (t = 16.85) in the level of catechin content was also detected between heterozygotes and homozygotes in the tea seed stock TS379. We found a polynomial relationship between the marker developed for CHS2 and catechin content in these tea samples with R2 = 0.9788. Moreover, PAL has less of a relationship with catechin content. Therefore, we recommend tea clones with heterozygous CAPS profiles for the gene CHS2+RcaI for the further improvement in these clones.

  18. Coupling of exothermic and endothermic hydrogen storage materials

    Energy Technology Data Exchange (ETDEWEB)

    Brooks, Kriston P.; Bowden, Mark E.; Karkamkar, Abhijeet J.; Houghton, Adrian Y.; Autrey, S. Thomas

    2016-08-01

    Chemical hydrogen storage (CHS) materials are a high-storage-density alternative to the gaseous compressed hydrogen currently used to provide hydrogen for fuel cell vehicles. One of the challenges of CHS materials is addressing the thermodynamic and kinetic barriers required to break the chemical bonds and release the hydrogen. Coupling CHS reactions that are endothermic and exothermic during the dehydrogenation can improve the system on-board energy efficiency and thermal control, making such materials viable. Acceptable coupling between reactions requires both thermodynamic and kinetics considerations. Models were developed to predict the reaction enthalpy and rate required to achieve high conversions for both reactions based on experimental measurements. These modeling results show that the efficiency of coupling of an exothermic and endothermic reaction is more sensitive the magnitude of the ratio of the exothermic and endothermic enthalpies than the ratio of the rates of the two steps. The modeling shows further that a slower rate of the endothermic step is desirable to permit sufficient heating of the reactor by the exothermic step. We look at two examples of a sequential and parallel reaction scheme and provide some of the first insight into the required temperature range to maximize the H2 release from 1,2-BN cyclohexane and indoline.

  19. Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

    Science.gov (United States)

    Zhu, Bin; Li, Xiuxia; Liu, Ying; Gao, Xiwu; Liang, Pei

    2017-01-01

    The diamondback moth (DBM), Plutella xylostella (L.), is one of the most serious cruciferous pests and has developed high resistance to most insecticides, including chlorantraniliprole. Previous studies have reported several protein-coding genes that involved in chlorantraniliprole resistance, but research on resistance mechanisms at the post-transcription level is still limited. In this study, a global screen of microRNAs (miRNAs) associated with chlorantraniliprole resistance in P. xylostella was performed. The small RNA libraries for a susceptible (CHS) and two chlorantraniliprole resistant strains (CHR, ZZ) were constructed and sequenced, and a total of 199 known and 30 novel miRNAs were identified. Among them, 23 miRNAs were differentially expressed between CHR and CHS, and 90 miRNAs were differentially expressed between ZZ and CHS, of which 11 differentially expressed miRNAs were identified in both CHR and ZZ. Using miRanda and RNAhybrid, a total of 1,411 target mRNAs from 102 differentially expressed miRNAs were predicted, including mRNAs in several groups of detoxification enzymes. The expression of several differentially expressed miRNAs and their potential targets was validated by qRT-PCR. The results may provide important clues for further study of the mechanisms of miRNA-mediated chlorantraniliprole resistance in DBM and other target insects. PMID:28098189

  20. Characteristics and Trends in Hypnotics Consumption in the Largest Health Care System in Israel

    Directory of Open Access Journals (Sweden)

    O. Marom

    2016-01-01

    Full Text Available Objectives. To quantify and characterize hypnotics consumption habits among adult patients insured by Clalit Health Services (CHS, the largest health care provider in Israel, in 2000 and 2010. Methods. A retrospective analysis of CHS computerized pharmacy records. Data were collected for all patients over the age of 18 years who were prescribed hypnotics in 2000 and in 2010. Results. Sleep medications were consumed by 8.7% of the adult CHS population in 2000 and by 9.6% in 2010. About one-quarter of consumers were treated for more than 6 months in both years. Multiple sleeping drugs were consumed more often in 2010 (45.2% than a decade before (22%. While in 2000 benzodiazepines accounted for 84.5% of hypnotics, in 2010 this was reduced to 73.7% (p<0.05. Of all patients treated for longer than 6 months only 11% in 2000 and 9% in 2010 required a dose escalation suggesting the absence of tolerance. Conclusions. Nine percent of the Israeli population consumes hypnotics. There is a major increase in prescription of combination of medications between 2000 and 2010, with an increase in Z class medications use and reduction in benzodiazepines. Most patients chronically treated did not escalate dosage, suggesting the absence of tolerance.

  1. Feeding a diet devoid of choline to lactating rodents restricts growth and lymphocyte development in offspring.

    Science.gov (United States)

    Lewis, E D; Goruk, S; Richard, C; Dellschaft, N S; Curtis, J M; Jacobs, R L; Field, C J

    2016-09-01

    The nutrient choline is necessary for membrane synthesis and methyl donation, with increased requirements during lactation. The majority of immune development occurs postnatally, but the importance of choline supply for immune development during this critical period is unknown. The objective of this study was to determine the importance of maternal supply of choline during suckling on immune function in their offspring among rodents. At parturition, Sprague-Dawley dams were randomised to either a choline-devoid (ChD; n 7) or choline-sufficient (ChS, 1 g/kg choline; n 10) diet with their offspring euthanised at 3 weeks of age. In a second experiment, offspring were weaned to a ChS diet until 10 weeks of age (ChD-ChS, n 5 and ChS-ChS, n 9). Splenocytes were isolated, and parameters of immune function were measured. The ChD offspring received less choline in breast milk and had lower final body and organ weight compared with ChS offspring (Pstimulation (lower stimulation index and less IFN-γ production) ex vivo (Pstimulation compared with cells from ChS-ChS (P<0·05). Our study suggests that choline is required in the suckling diet to facilitate immune development, and choline deprivation during this critical period has lasting effects on T cell function later in life.

  2. Bibliometric analysis about the scientific production on the Social Skills Field

    Directory of Open Access Journals (Sweden)

    Eliane Colepicolo

    2015-05-01

    Full Text Available Objective. This research consisted to contribute to the systematization of the theoretical and practical field of Social Skills, here called Social Skills Field (SSF. Method. A survey of a representative sample of CHS publications metadata extracted from the 15 scientific and technical literature databases in Psychology available in the CAPES Journals Portal was conducted. After treatment and refinement, this sample resulted in a corpus of metadata 25,409 scientific articles, which is a set of metadata publications, from fields such as author, title, subject, abstract, among others. The corpus of metadata stored in a database allowed the creation of a series of data crossing, resulting in bibliometric indicators. Results. As a result, we have: the SS Corpus, published on the Internet, which enables the inclusion of new metadata and the recovery of almost all scientific articles of the field; 2 bibliometric indicators in CHS, which may offer subsidies for the development and expansion of the field. Conclusions. Bibliometric indicators of the CHS show a representative production growth of social skills in the 1970s. Research on SS is mainly conducted by researchers from the United States, Brazil, United Kingdon and Denmark. The main subject studied are "social skills", "interpersonal relations", "social competence", "empathy", "assertividade", "assertiveness", "children", "adult", "college students"; and the major journals are: Journal of Personality and Social Psychology; Psychological Reports; Journal of Autism and Developmental Disorders and Child Development.

  3. Competency requirements for middle and senior managers in community health services.

    Science.gov (United States)

    Liang, Zhanming; Howard, Peter F; Koh, Lee C; Leggat, Sandra

    2013-01-01

    The Australian health system has been subjected to rapid changes in the last 20 years to meet increasingly unmet health needs. Improvement of the efficiency and comprehensiveness of community-based services is one of the solutions to reducing the increasing demand for hospital care. Competent managers are one of the key contributors to effective and efficient health service delivery. However, the understanding of what makes a competent manager, especially in the community health services (CHS), is limited. Using an exploratory and mixed-methods approach, including focus group discussions and an online survey, this study identified five key competencies required by senior and mid-level CHS managers in metropolitan, regional and rural areas of Victoria: Interpersonal, communication qualities and relationship management; Operations, administration and resource management; Knowledge of the health care environment; Leading and managing change; and Evidence-informed decision-making. This study confirms that core competencies do exist across different management levels and improves our understanding of managerial competency requirements for middle to senior CHS managers, with implications for current and future health service management workforce development.

  4. The Effect of the Transition to Home Monitoring for the Diagnosis of OSAS on Test Availability, Waiting Time, Patients’ Satisfaction, and Outcome in a Large Health Provider System

    Directory of Open Access Journals (Sweden)

    Ahmad Safadi

    2014-01-01

    Full Text Available During 2009, the Haifa district of Clalit Health Services (CHS has switched from in-lab polysomnography (PSG to home studies for the diagnosis of obstructive sleep apnea (OSA. We assessed the effects of this change on accessibility, waiting time, satisfaction, costs, and CPAP purchase by the patients. Data regarding sleep studies, CPAP purchase, and waiting times were collected retrospectively from the computerized database of CHS. Patients’ satisfaction was assessed utilizing a telephone questionnaire introduced to a randomized small sample of 70 patients. Comparisons were made between 2007 and 2008 (in-lab PSGs and 2010 and 2011 (when most studies were ambulatory. Of about 650000 insured individuals in the Haifa district of CHS, 1471 sleep studies were performed during 2007-2008 compared to 2794 tests during 2010-2011. The average waiting time was 9.9 weeks in 2007-2008 compared to 1.1 weeks in 2010-2011 (P<0.05. 597 CPAPs were purchased in 2007-2008 compared to 831 in 2010-2011. The overall patients’ satisfaction was similar, but discomfort tended to be higher in the in-laboratory group (4.1 vs 2.7 in a scale of 0–10; P=0.11. Switching to ambulatory diagnosis improved the test accessibility and reduced the waiting times. Patients’ satisfaction remained similarly high. The total direct cost of OSA management was reduced.

  5. In vitro and in vivo co-culture of chondrocytes and bone marrow stem cells in photocrosslinked PCL-PEG-PCL hydrogels enhances cartilage formation.

    Science.gov (United States)

    Ko, Chao-Yin; Ku, Kuan-Lin; Yang, Shu-Rui; Lin, Tsai-Yu; Peng, Sydney; Peng, Yu-Shiang; Cheng, Ming-Huei; Chu, I-Ming

    2016-10-01

    Chondrocytes (CH) and bone marrow stem cells (BMSCs) are sources that can be used in cartilage tissue engineering. Co-culture of CHs and BMSCs is a promising strategy for promoting chondrogenic differentiation. In this study, articular CHs and BMSCs were encapsulated in PCL-PEG-PCL photocrosslinked hydrogels for 4 weeks. Various ratios of CH:BMSC co-cultures were investigated to identify the optimal ratio for cartilage formation. The results thus obtained revealed that co-culturing CHs and BMSCs in hydrogels provides an appropriate in vitro microenvironment for chondrogenic differentiation and cartilage matrix production. Co-culture with a 1:4 CH:BMSC ratio significantly increased the synthesis of GAGs and collagen. In vivo cartilage regeneration was evaluated using a co-culture system in rabbit models. The co-culture system exhibited a hyaline chondrocyte phenotype with excellent regeneration, resembling the morphology of native cartilage. This finding suggests that the co-culture of these two cell types promotes cartilage regeneration and that the system, including the hydrogel scaffold, has potential in cartilage tissue engineering. Copyright © 2013 John Wiley & Sons, Ltd.

  6. Deflections of Fast Coronal Mass Ejections and the Properties of Associated Solar Energetic Particle Events

    Science.gov (United States)

    Kahler, S. W.; Akiyama, S.; Gopalswamy, N.

    2012-01-01

    The onset times and peak intensities of solar energetic particle (SEP) events at Earth have long been thought to be influenced by the open magnetic fields of coronal holes (CHs). The original idea was that a CH lying between the solar SEP source region and the magnetic footpoint of the 1 AU observer would result in a delay in onset and/or a decrease in the peak intensity of that SEP event. Recently, Gopalswamy et al. showed that CHs near coronal mass ejection (CME) source regions can deflect fast CMEs from their expected trajectories in space, explaining the appearance of driverless shocks at 1 AU from CMEs ejected near solar central meridian (CM). This suggests that SEP events originating in CME-driven shocks may show variations attributable to CH deflections of the CME trajectories. Here, we use a CH magnetic force parameter to examine possible effects of CHs on the timing and intensities of 41 observed gradual E approx 20 MeV SEP events with CME source regions within 20 deg. of CM. We find no systematic CH effects on SEP event intensity profiles. Furthermore, we find no correlation between the CME leading-edge measured position angles and SEP event properties, suggesting that the widths of CME-driven shock sources of the SEPs are much larger than the CMEs. Independently of the SEP event properties, we do find evidence for significant CME deflections by CH fields in these events

  7. Biocompatibility and Stability of Polysaccharide Polyelectrolyte Complexes Aimed at Respiratory Delivery

    Directory of Open Access Journals (Sweden)

    Susana Rodrigues

    2015-08-01

    Full Text Available Chitosan (CS and chondroitin sulfate (CHS are natural polymers with demonstrated applicability in drug delivery, while nanoparticles are one of the most explored carriers for transmucosal delivery of biopharmaceuticals. In this work we have prepared CS/CHS nanoparticles and associated for the first time the therapeutic protein insulin. Fluorescein isothiocyanate bovine serum albumin (FITC-BSA was also used to enable comparison of behaviors regarding differences in molecular weight (5.7 kDa versus 67 kDa. Nanoparticles of approximately 200 nm and positive zeta potential around +20 mV were obtained. These parameters remained stable for up to 1 month at 4 °C. Proteins were associated with efficiencies of more than 50%. The release of FITC-BSA in PBS pH 7.4 was more sustained (50% in 24 h than that of insulin (85% in 24 h. The biocompatibility of nanoparticles was tested in Calu-3 and A549 cells by means of three different assays. The metabolic assay MTT, the determination of lactate dehydrogenase release, and the quantification of the inflammatory response generated by cell exposure to nanoparticles have indicated an absence of overt toxicity. Overall, the results suggest good indications on the application of CS/CHS nanoparticles in respiratory transmucosal protein delivery, but the set of assays should be widened to clarify obtained results.

  8. Cloning and Expression Analysis of Key Enzymes Genes in Biosynthesis of Soybean Isoflavones%大豆异黄酮合成关键酶基因的克隆及表达分析

    Institute of Scientific and Technical Information of China (English)

    钱丹丹; 龚德顺; 焦丽; 翟莹; 张海军; 李景文; 王英; 王庆钰

    2011-01-01

    采用RT-PCR法克隆了大豆异黄酮生物合成途径中查尔酮合酶(Chalcone synthase,CHS)和查尔酮异构酶(Chalcone Isomerase,CHI)2个关键酶基因,并利用SOE-PCR技术成功将2个基因融合,将其均构建成原核表达载体,在大肠杆菌中进行了初步的表达研究,为进一步研究CHS和CHI 2种关键酶的功能奠定了基础.同时构建了真核表达载体,采用农杆菌转化法侵染大豆子叶节,获得了转基因抗性植株.%Chalcone Synthase(CHS)and Chalcone Isomerase(CHI) are two key enzymes in soybean isoflavones biosynthesis pathway,the cDNAs of CHS and CHI were cloned using RT-PCR method and these two genes were fused using SOE-PCR,then constructed E. Coli expression vectors to study the expression. The eukaryocyte expression vectors were also constructed and transferred into soybean cotyledonary nodes using Agrobacterium-mediated transformation,and then obtained the resistant trans-genic plants.

  9. Primary Spinal Chondrosarcoma: Radiologic Findings with Pathologic Correlation

    Energy Technology Data Exchange (ETDEWEB)

    Lloret, I.; Server, A. [The Norwegian Radium Hospital, Oslo (Norway). Depts. of Radiology and Pathology; Bjerkehagen, B. [Ullevaal Univ. Hospital, Oslo (Norway). Dept. of Neuroradiology

    2006-02-15

    Purpose: To describe the radiologic appearance of the four types of primary spinal chondrosarcoma (CHS) (conventional intramedullary, juxtacortical, clear cell, and mesenchymal) and to correlate with histopathologic findings. Material and Methods: A retrospective review was carried out of 5 patients with histopathologically confirmed primary spinal CHS; 3 F and 2 M ranging in age between 27 and 66 years (mean 40.2; median 39). Charts, conventional radiographs, computed tomography scans, and magnetic resonance images were reviewed. All the patients underwent surgical excision, followed by postoperative chemotherapy (1 patient) and radiotherapy (3 patients). Follow-up was available for all patients but one. The mean follow-up was 42 months (14-120 months). Histopathological specimens for all patients were available for review. Results: Vertebral column distribution was 3 thoracic (60%), 1 cervical (20%), and 1 lumbar (20%). Neurological deficits were present in 3 (60%) cases. The radiological appearance of the four types of primary spinal CHS varies with specific lesion type. Imaging findings suggest diagnosis of the conventional intramedullary and juxtacortical types. While the clear cell and mesenchymal types show some distinctive features, these do not allow confident radiologic diagnosis. Conclusion: The radiologist must be aware of imaging features of these tumors in order to improve diagnostic accuracy, treatment planning, and prognosis.

  10. Comparison of predictive value between two kinds of score system in hospital mortality of pneumonia patients%两种基础疾病评分预测肺炎患者预后的比较

    Institute of Scientific and Technical Information of China (English)

    崔云亮; 张树柳; 田昭涛; 陈德昌; 林兆奋

    2016-01-01

    目的 比较慢性健康状态评分(chronic health score,CHS)和查尔森合并症指数(Charlson's weighted index of comorbidities,WIC)对ICU肺部感染患者住院死亡风险的影响.方法 回顾性分析多中心2013年9月至2014年9月肺炎患者的临床资料;记录性别、年龄、既往基础疾病、是否严重脓毒症;计算入院时WIC、入院后24h急性生理与慢性健康状况(APACHE)评分Ⅱ,包括CHS、急性生理状况评分(acute physiology score,APS)和脓毒症相关性器官功能衰竭评分(sepsis related organ failure assessment,SOFA).结果 在293名入组患者中,男性195例(66.6%),女性98例(33.4%),存活197例(67.2%),死亡96例(32.8%).年龄(62.5±17.0)岁.与存活组比较,死亡组的WIC评分较高.而两组的CHS评分比较无统计学意义.随着WIC和CHS分值的增高,患者的病死率呈增高趋势.单因素Logistic回归分析显示年龄、APACHEⅡ、SOFA、APS、CHS、WIC、是否严重脓毒症、基础疾病中Ⅱ型糖尿病、卒中、充血性心力衰竭与患者的预后相关.CHS(方程1)和WIC评分(方程2)分别联合入院急性生理状况评分及年龄等因素建立方程行多因素Logistic回归分析提示年龄、APS、CHS、WIC、是否严重脓毒症与患者的28 d预后独立相关.CHS评分的受试者工作曲线下面积(AUC)为0.557,95% CI:(0.49~0.623)低于(P =0.021 7,Z=2.296) WIC评分的AUC =0.649 95%CI:(0.586 ~0.712),联合多变量建立方程后的AUC明显优于APACHEⅡ评分(P <0.001).结论 WIC评分是预测ICU肺炎患者住院病死率的独立风险因素;WIC评分较CHS评分可以更好的评价基础疾病对患者病死率的影响.%Objective To compare the value in predicting hospital mortality of pneumonia patients between Charlson's weighted index of comorbidities (WIC) and chronic health score (CHS),in Intensive Care Units (ICU).Methods The clinical data of pneumonia patients collected from September 2013 to September 2014 in ICU of

  11. Dsp rul: a spontaneous mouse mutation in desmoplakin as a model of Carvajal-Huerta syndrome.

    Science.gov (United States)

    Herbert Pratt, C; Potter, Christopher S; Fairfield, Heather; Reinholdt, Laura G; Bergstrom, David E; Harris, Belinda S; Greenstein, Ian; Dadras, Soheil S; Liang, Bruce T; Schofield, Paul N; Sundberg, John P

    2015-04-01

    Studies of spontaneous mutations in mice have provided valuable disease models and important insights into the mechanisms of human disease. Ruffled (rul) is a new autosomal recessive mutation causing abnormal hair coat in mice. The rul allele arose spontaneously in the RB156Bnr/EiJ inbred mouse strain. In addition to an abnormal coat texture, we found diffuse epidermal blistering, abnormal electrocardiograms (ECGs), and ventricular fibrosis in mutant animals. Using high-throughput sequencing (HTS) we found a frameshift mutation at 38,288,978bp of chromosome 13 in the desmoplakin gene (Dsp). The predicted mutant protein is truncated at the c-terminus and missing the majority of the plakin repeat domain. The phenotypes found in Dsp(rul) mice closely model a rare human disorder, Carvajal-Huerta syndrome. Carvajal-Huerta syndrome (CHS) is a rare cardiocutaneous disorder that presents in humans with wooly hair, palmoplantar keratoderma and ventricular cardiomyopathy. CHS results from an autosomal recessive mutation on the 3' end of desmoplakin (DSP) truncating the full length protein. The Dsp(rul) mouse provides a new model to investigate the pathogenesis of CHS, as well as the underlying basic biology of the adhesion molecules coded by the desmosomal genes.

  12. Toll-like receptor 3 increases allergic and irritant contact dermatitis.

    Science.gov (United States)

    Nakamura, Naomi; Tamagawa-Mineoka, Risa; Ueta, Mayumi; Kinoshita, Shigeru; Katoh, Norito

    2015-02-01

    There is increasing recognition of the role of Toll-like receptor 3 (TLR3) in noninfectious inflammatory diseases, but the function of TLR3 in inflammatory skin diseases is unclear. We investigated the functions of TLR3 in allergic and irritant contact dermatitis (ICD). The contact hypersensitivity (CHS) response was lower in Toll-like receptor 3 knockout (Tlr3 KO) mice, and was greater in TLR3 transgenic (Tg) mice than in wild-type (WT) mice after challenge with 2,4,6-trinitro-1-chlorobenzene. Adoptive transfer of immunized lymph node cells from Tlr3 KO mice induced CHS in WT recipients. In contrast, adoptive transfer of those from WT mice did not fully induce CHS in Tlr3 KO recipients. The ICD reaction following croton oil application was lower in Tlr3 KO mice, and was greater in TLR3 Tg mice than in WT mice. Maturation, migration, and antigen presentation of dendritic cells and proliferation of lymphocytes between WT mice and Tlr3 KO mice were comparable. These results show that TLR3 enhances antigen-independent skin inflammation in the elicitation phase of allergic contact dermatitis and in ICD.

  13. Drosophila cuticular hydrocarbons revisited: mating status alters cuticular profiles.

    Directory of Open Access Journals (Sweden)

    Claude Everaerts

    Full Text Available Most living organisms use pheromones for inter-individual communication. In Drosophila melanogaster flies, several pheromones perceived either by contact/at a short distance (cuticular hydrocarbons, CHs, or at a longer distance (cis-vaccenyl acetate, cVA, affect courtship and mating behaviours. However, it has not previously been possible to precisely identify all potential pheromonal compounds and simultaneously monitor their variation on a time scale. To overcome this limitation, we combined Solid Phase Micro-Extraction with gas-chromatography coupled with mass-spectrometry. This allowed us (i to identify 59 cuticular compounds, including 17 new CHs; (ii to precisely quantify the amount of each compound that could be detected by another fly, and (iii to measure the variation of these substances as a function of aging and mating. Sex-specific variation appeared with age, while mating affected cuticular compounds in both sexes with three possible patterns: variation was (i reciprocal in the two sexes, suggesting a passive mechanical transfer during mating, (ii parallel in both sexes, such as for cVA which strikingly appeared during mating, or (iii unilateral, presumably as a result of sexual interaction. We provide a complete reassessment of all Drosophila CHs and suggest that the chemical conversation between male and female flies is far more complex than is generally accepted. We conclude that focusing on individual compounds will not provide a satisfactory understanding of the evolution and function of chemical communication in Drosophila.

  14. An Energy-Efficient Cluster-Head Selection Protocol for Energy-Constrained Wireless Sensor Networks

    Science.gov (United States)

    Hao, Peng; Qiu, Wanzhi; Evans, Rob

    In clustered wireless sensor networks, cluster-heads (CHs) are the key. And the CH selection methods can affect the efficiency of the cluster and even influence the lifetime of an energy-constrained network. In this paper, we propose the Energy-consumption-cycle based Cluster-heads Selection (ECHS) protocol. ECHS selects CHs via two phases. In the first phase, CH candidates are chosen based on an adaptable energy threshold and the status of neighbor nodes. In the second phase, each candidate computes its own Energy Consumption Cycles (ECC), which is relevant to the CH candidate's residual energy and the energy consumption when communicating with its neighbor nodes. The ECC actually evaluates the lifetime of the potential cluster. Hence the CH candidate with both higher residual energy and higher ECC has more possibility to be the CH. By comparing ECC, the suitable CHs can be obtained. Simulation results illustrate that ECHS protocol prolongs the network lifetime and also increases the amount of data received at BS.

  15. The Liquid Metallic Hydrogen Model of the Sun and the Solar Atmosphere VIII. "Futile" Processes in the Chromosphere (Letters to Progress in Physics

    Directory of Open Access Journals (Sweden)

    Robitaille L.

    2014-01-01

    Full Text Available In the liquid metallic hydrogen solar model (LMHSM, the chr omosphere is the site of hydrogen condensation (P.M. Robitaille. The Liquid Metall ic Hydrogen Model of the Sun and the Solar Atmosphere IV. On the Nature of the Chromosp here. Progr. Phys. , 2013, v. 3, L15–L21. Line emission is associated with the di ssipation of energy from condensed hydrogen structures, CHS. Previously considere d reactions resulted in hy- drogen atom or cluster addition to the site of condensation. In this work, an additional mechanism is presented, wherein atomic or molecular specie s interact with CHS, but do not deposit hydrogen. These reactions channel heat away f rom CHS, enabling them to cool even more rapidly. As a result, this new class of proce sses could complement true hydrogen condensation reactions by providing an auxil iary mechanism for the re- moval of heat. Such ‘futile’ reactions lead to the formation of activated atoms, ions, or molecules and might contribute to line emission from such sp ecies. Evidence that com- plimentary ‘futile’ reactions might be important in the chr omosphere can be extracted from lineshape analysis.

  16. Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development.

    Science.gov (United States)

    Jiao, Yun; Ma, Rui-juan; Shen, Zhi-jun; Yan, Juan; Yu, Ming-liang

    2014-09-01

    The blood-flesh peach has become popular in China due to its attractive anthocyanin-induced pigmentation and antioxidant properties. In this study, we investigated the molecular mechanisms underlying anthocyanin accumulation by examining the expression of nine genes of the anthocyanin biosynthesis pathway found in the peach mesocarp. Expression was measured at six developmental stages in fruit of two blood-flesh and one white-flesh peach cultivars, using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results show that the expression of the chalcone synthase (CHS) gene was closely related to anthocyanin accumulation in both of the blood-flesh peaches. In the white-flesh peach, we found that the transcription level of phenylalanine ammonia-lyase (PAL) during fruit development was much lower than that in the blood-flesh peach, even though all other genes of the anthocyanin biosynthesis pathway were highly expressed, suggesting that the PAL gene may be limiting in anthocyanin production in the white-flesh peach. Moreover, the transcription levels of the CHS and UDP-glucose-flavonoid 3-O-glucosyltransferase (UFGT) genes were markedly up-regulated at three days after bag removal (DABR) in the blood-flesh peach, suggesting that CHS and UFGT are the key genes in the process of anthocyanin biosynthesis for both of the blood-flesh peaches. The present study will be of great help in improving understanding of the molecular mechanisms involved in anthocyanin accumulation in blood-flesh peaches.

  17. Questioning the concept of risk in ethical guidelines for research in the humanities and social sciences in Public Health.

    Science.gov (United States)

    Bosi, Maria Lúcia Magalhães

    2015-09-01

    This article discusses the use of the concept of risk in ethical guidelines directed to research in the humanities and social sciences (CHS), suggesting an alternative to that concept. In Public Health field (PH), risk assumes a peculiar semantics, closely linked to the idea of calculation and predictability, according to the disciplinary bases that support it. This circumstance makes incongruous its use in initiatives justified precisely by strong distinctions between biomedical and social research, as ilustrated by specific guidelines for CHS, especially to the qualitative approach. The authors do not seek to redefine risk, operating a conceptual transit, but to sustain an effective conceptual distance within these specific guidelines, keeping congruence with the objectives pursued by its construction. Taking risk in the quantitative sense, still hegemonic in PH, overlooks important dimensions, reifying the use of this concept in situations where uncertainty, unpredictability, intersubjectivity inherent to the processes beyond the calculation and measurement, as in the case of a significant portion of the research in CHS. Alternatively, it is suggested to replace the expression level of risk, as also appears in Brazilian resolutions.

  18. Identification and characterization of enhancer-blocking insulators to reduce retroviral vector genotoxicity.

    Directory of Open Access Journals (Sweden)

    Amy C Groth

    Full Text Available The chromatin insulator cHS4 can reduce silencing chromosomal position effects and genotoxicity associated with integrating viral vectors. However, the fully active version of this element can also reduce vector titers and is only partially effective. In order to identify alternatives to cHS4, we developed a functional lentiviral vector-based reporter screen for enhancer-blocking insulators. Using this system, we screened candidate sequences that were initially identified by chromatin profiling for binding by CTCF and for DNase hypersensitivity. All 12 analyzed candidates blocked enhancer-promoter activity. The enhancer-blocking activity of the top two candidates was confirmed in two complementary plasmid-based assays. Studies in a gammaretroviral reporter vector indicated these two candidates have little to no effect on vector titers, and do not diminish vector expression in primary mouse bone marrow cultures. Subsequent assessment in a mouse in vivo tumor formation model demonstrated that both candidates reduced the rate of gammaretroviral vector-mediated genotoxicity as effectively as the cHS4 insulator. In summary, we have developed a novel lentiviral vector-based method of screening candidate elements for insulator activity, and have used this method to identify two new insulator elements capable of improving the safety of retroviral vectors without diminishing vector titers or expression. These findings expand the limited arsenal of insulators functionally validated to reduce the rate of retroviral vector-mediated genotoxicity.

  19. Cuticular hydrocarbons corroborate the distinction between lowland and highland Natal fruit fly (Tephritidae, Ceratitis rosa) populations

    Science.gov (United States)

    Vaníčková, Lucie; Břízová, Radka; Pompeiano, Antonio; Ekesi, Sunday; Meyer, Marc De

    2015-01-01

    Abstract The cuticular hydrocarbons (CHs) and morphology of two Ceratitis rosa Karsch (Diptera: Tephritidae) populations, putatively belonging to two cryptic taxa, were analysed. The chemical profiles were characterised by two-dimensional gas chromatography with mass spectrometric detection. CHs of Ceratitis rosa that originated from the lowlands and highlands of Kenya comprised of n-alkanes, monomethylalkanes, dimethylalkanes and unsaturated hydrocarbons in the range of the carbon backbone from C14 to C37. Hydrocarbons containing C29, C31, C33 and C35 carbon atoms predominated in these two populations. 2-Methyltriacontane was the predominant compound in both populations. Quantitative differences in the distribution of hydrocarbons of different chain lengths, mainly the C22, C32, C33 and C34 compounds of these two populations, were observed despite indistinct qualitative differences in these hydrocarbons. Morphological analyses of male legs confirmed that the flies belong to different morphotypes of Ceratitis rosa previously labelled as R1 and R2 for lowland and highland populations, respectively. A statistical analysis of the CH compositions of the putative R1 and R2 species showed distinct interspecific identities, with several CHs specific for each of the lowland and highland populations. This study supports a hypothesis that the taxon Ceratitis rosa consists of at least two biological species. PMID:26798275

  20. Photodynamic therapy affects the expression of IL-6 and IL-10 in vivo

    Science.gov (United States)

    Gollnick, Sandra O.; Musser, David A.; Henderson, Barbara W.

    1998-05-01

    Photodynamic therapy (PDT), which can effectively destroy malignant tissue, also induces a complex immune response which potentiates anti-tumor immunity, but also inhibits skin contact hypersensitivity (CHS) and prolongs skin graft survival. The underlying mechanisms responsible for these effects are poorly understood, but are likely to involve meditation by cytokines. We demonstrate in a BALB/c mouse model that PDT delivered to normal and tumor tissue in vivo causes marked changes in the expression of cytokines interleukin (IL)-6 and IL-10. IL-6 mRNA and protein are rapidly and strongly enhanced in the PDT treated EMT6 tumor. Previous studies have shown that intratumoral injection of IL- 6 or transduction of the IL-6 gene into tumor cells can enhance tumor immunogenicity and inhibit tumor growth in experimental murine tumor systems. Thus, PDT may enhance local anti-tumor immunity by up-regulating IL-6. PDT also results in an increase in IL-10 mRNA and protein in the skin. The same PDT regime which enhances IL-10 production in the skin has been shown to strongly inhibit the CHS response. The kinetics of IL-10 expression coincide with the known kinetics of PDT induced CHS suppression and we propose that the enhanced IL-10 expression plays a role in the observed suppression of cell mediated responses seen following PDT.

  1. Immunosuppressive Effect of Litsea cubeba L. Essential Oil on Dendritic Cell and Contact Hypersensitivity Responses

    Directory of Open Access Journals (Sweden)

    Hsin-Chun Chen

    2016-08-01

    Full Text Available Litsea cubeba L., also named as Makauy, is a traditional herb and has been used as cooking condiment or tea brewing to treat diseases for aborigines. The present study was undertaken to explore the chemical compositions of the fruit essential oil of L. cubeba (LCEO and the immunomodulatory effect of LCEO on dendritic cells and mice. The LCEO was analyzed using gas chromatography (GC and gas chromatography/mass spectrometry (GC/MS with direct injection (DI/GC or headspace-solid phase microextraction (HS-SPME/GC. In total, 56 components were identified, of which 48 were detected by DI/GC and 49 were detected by HS-SPME/GC. The principal compounds were citral (neral and geranial. An immunosuppressive activity of LCEO was investigated with bone marrow-derived dendritic cells (DCs which have a critical role to trigger the adaptive immunity. Additionally, the inhibitory effect of LCEO on immune response was elucidated by performing the contact hypersensitivity (CHS responses in mice. Our results clearly showed that LCEO decreases the production of TNF-α and cytokine IL-12 in a dose-dependent manner in lipopolysaccharide (LPS-stimulated DCs. CHS response and the infiltrative T cells were inhibited in the tested ears of the mice co-treated with LCEO. We demonstrate, for the first time, that the LCEO mainly containing citral exhibits an immunosuppressive effect on DCs and mice, indicating that LCEO can potentially be applied in the treatment of CHS, inflammatory diseases, and autoimmune diseases.

  2. 3D-FEM electrical-thermal-mechanical analysis and experiment of Si-based MEMS infrared emitters

    Science.gov (United States)

    Wang, Xiang; Wang, Na; Chen, Ran-Bin; San, Hai-Sheng; Chen, Xu-Yuan

    2016-11-01

    Designs, simulations, and fabrications of silicon-based MEMS infrared (IR) emitters for gas sensing application are presented. A 3D finite element method (3D-FEM) was used to analyze the coupled electrical-thermal-mechanical properties of a bridge hotplate structure (BHS) IR emitter and closed hotplate structure (CHS) IR emitter using Joule heating and thermal expansion models of COMSOL™. The IR absorptions of n- and p-silicon were calculated for the design of self-heating structure. The BHS and CHS IR emitters were fabricated synchronously using micro-electromechanical systems technology for a direct performance comparison. Both types of IR emitters were characterized by electrical and optical measurements. The experimental results show that BHS IR emitters have higher radiation density, lower power consumption, and faster frequency-response than CHS IR emitters due to the use of a thermal isolation structure and self-heating structure. Meanwhile, the simulated results agree well with the corresponding measured results, which indicate that the 3D-FEM-model is effective and can be used in the optimal design of electro-thermal devices.

  3. Coupling of exothermic and endothermic hydrogen storage materials

    Science.gov (United States)

    Brooks, Kriston P.; Bowden, Mark E.; Karkamkar, Abhijeet J.; Houghton, Adrian Y.; Autrey, S. Thomas

    2016-08-01

    Chemical hydrogen storage (CHS) materials are a high-storage-density alternative to the gaseous compressed hydrogen currently used to provide hydrogen for fuel cell vehicles. One of the challenges of CHS materials is addressing the energy barriers required to break the chemical bonds and release the hydrogen. Coupling CHS reactions that are endothermic and exothermic during dehydrogenation can improve onboard energy efficiency and thermal control for the system, making such materials viable. Acceptable coupling between reactions requires both thermodynamic and kinetic considerations. In this work, models were developed to predict the reaction enthalpy and rate required to achieve high conversions for both reactions based on experimental measurements. Modeling results show that the coupling efficiency of exothermic and endothermic reactions is more sensitive to the ratio of the exothermic and endothermic enthalpies than to the ratio of the rates of the two steps. Modeling results also show that a slower endothermic step rate is desirable to permit sufficient heating of the reactor by the exothermic step. We look at two examples of a sequential and parallel reaction scheme and provide some of the first published insight into the required temperature range to maximize the hydrogen release from 1,2-BN cyclohexane and indoline.

  4. Thomson scattering diagnostic on the Compact Toroidal Hybrid Experiment

    Science.gov (United States)

    Traverso, Peter; Maurer, D. A.; Ennis, D. A.; Hartwell, G. J.

    2016-10-01

    A Thomson scattering system is being commissioned for the non-axisymmetric plasmas of the Compact Toroidal Hybrid (CTH), a five-field period current-carrying torsatron. The system takes a single point measurement at the magnetic axis to both calibrate the two- color soft x-ray Te system and serve as an additional diagnostic for the V3FIT 3D equilibrium reconstruction code. A single point measurement will reduce the uncertainty in the reconstructed peak pressure by an order of magnitude for both current-carrying plasmas and future gyrotron-heated stellarator plasmas. The beam, generated by a frequency doubled Continuum 2 J, Nd:YaG laser, is passed vertically through an entrance Brewster window and a two-aperture optical baffle system to minimize stray light. The beam line propagates 8 m to the CTH device mid-plane with the beam diameter < 3 mm inside the plasma volume. Thomson scattered light is collected by two adjacent f/2 plano-convex condenser lenses and focused onto a custom fiber bundle. The fiber is then re-bundled and routed to a Holospec f/1.8 spectrograph to collect the red-shifted scattered light from 535-565 nm. The system has been designed to measure plasmas with core Te of 100 to 200 eV and densities of 5 ×1018 to 5 ×1019 m-3. Work supported by USDOE Grant DE-FG02-00ER54610.

  5. Research on stellarator-mirror fission-fusion hybrid

    Science.gov (United States)

    Moiseenko, V. E.; Kotenko, V. G.; Chernitskiy, S. V.; Nemov, V. V.; Ågren, O.; Noack, K.; Kalyuzhnyi, V. N.; Hagnestål, A.; Källne, J.; Voitsenya, V. S.; Garkusha, I. E.

    2014-09-01

    The development of a stellarator-mirror fission-fusion hybrid concept is reviewed. The hybrid comprises of a fusion neutron source and a powerful sub-critical fast fission reactor core. The aim is the transmutation of spent nuclear fuel and safe fission energy production. In its fusion part, neutrons are generated in deuterium-tritium (D-T) plasma, confined magnetically in a stellarator-type system with an embedded magnetic mirror. Based on kinetic calculations, the energy balance for such a system is analyzed. Neutron calculations have been performed with the MCNPX code, and the principal design of the reactor part is developed. Neutron outflux at different outer parts of the reactor is calculated. Numerical simulations have been performed on the structure of a magnetic field in a model of the stellarator-mirror device, and that is achieved by switching off one or two coils of toroidal field in the Uragan-2M torsatron. The calculations predict the existence of closed magnetic surfaces under certain conditions. The confinement of fast particles in such a magnetic trap is analyzed.

  6. Design and implementation of a multichannel millimeter wave interferometer for the Compact Toroidal Hybrid experiment

    Energy Technology Data Exchange (ETDEWEB)

    Miller, M. C.; Hanson, J. D.; Hartwell, G. J.; Knowlton, S. F.; Maurer, D. A.; Stevenson, B. A. [Physics Department, Auburn University, Auburn, Alabama 36849 (United States)

    2012-10-15

    A three-channel 1 mm wave interferometer has been designed, assembled, and installed on the Compact Toroidal Hybrid torsatron (CTH). The interferometer design makes novel use of a subharmonic mixer for detection, which simplifies alignment. It employs a single electronically tunable source that is repetitively chirped using a sawtooth waveform of frequency up to 1 MHz. The 15.25 GHz drive oscillator is multiplied in two stages to 122 GHz before a final doubler stage brings it to 244 GHz. Local oscillator (LO) power at 122 GHz is directed through waveguide to the LO input of the subharmonic mixer of each viewing chord, simplifying alignment. Phase detection is performed by directly digitizing the amplified mixer outputs at 50 MHz and processing them with a software algorithm. Initial measurements made with the central chord of the new interferometer agree with those from the existing 4 mm system at low densities. The 1 mm system performs well in current-driven discharges reaching densities over 10{sup 19} m{sup -3}, whereas the lower frequency interferometer is found to be less reliable due to loss of fringes. This is a critical improvement for experiments studying the onset, avoidance, and vacuum magnetic transform dependence of disruptions in the CTH device.

  7. Curvature dependance of blob dynamics in TJ-K

    Energy Technology Data Exchange (ETDEWEB)

    Garland, Stephen; Ramisch, Mirko [Institut fuer Grenzflaechenverfahrenstechnik und Plasmatechnologie, Universitaet Stuttgart (Germany); Fuchert, Golo [Institut Jean Lamour, Universite de Lorraine (France)

    2014-07-01

    Turbulent transport in the scrape-off layer (SOL) is an important area of investigation in magnetic confinement fusion research. Relatively dense and hot, field-aligned, filament-like structures (blobs) have been observed to propagate radially through the SOL in many fusion devices, and contribute significantly to SOL transport. The torsatron TJ-K operates with a low-temperature plasma, allowing Langmuir probe measurements in the entire plasma volume. Despite the low temperature, investigations are relevant to fusion research due to dimensionless plasma parameters similar to those in the edge region of fusion plasmas. Analytical blob models link blob velocity in the SOL to blob polarisation, which can be driven by magnetic field line curvature. In TJ-K, average blob dynamics can be studied in detail using a 2D movable probe and a conditional averaging technique. In addition, a fast camera can be used to supplement probe data, and provide information on individual blob trajectories. With these tools, the connection between magnetic field line curvature and the poloidal component of blob velocity has been studied. Taking into account background E x B flows, initial investigations suggest a correlation between the poloidal component of blob velocity and averaged geodesic magnetic field line curvature.

  8. Investigation of turbulent transport and shear flows in the Edge of toroidal plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Birkenmeier, G.; Koehn, A.; Manz, P.; Nold, B.; Stroth, U. [Institut fuer Plasmaforschung, Universitaet Stuttgart, Stuttgart (Germany); Happel, T. [Lab. Nacional de Fusion, Asociacion EURATOM-CIEMAT, Madrid (Spain); Mahdizadeh, N. [ABB Switzerland Ltd. Corporate Research, Baden-Daettwil (Switzerland); Wilcox, R.; Anderson, D.T. [HSX Plasma Lab., University of Wisconsin, Madison, Wisconsin (United States); Ramisch, M.

    2010-08-15

    Intense Langmuir-probe measurements were carried out in the toroidal low-temperature plasma of the torsatron TJ-K in order to investigate the origin and dynamics of intermittent transport events, so-called blobs, at the transition from closed to open field lines. The statistical properties of the fluctuations at the plasma boundary agree with observations made in fusion edge plasmas. Blobs were found to be generated locally through a change in turbulence drive across the separatrix. The non-linear spectral energy transfer from small-scale fluctuations into large-scale flows was measured with a 128-probe array. The results point to the transfer being a key loss channel for turbulence energy leading to a reduction in turbulent transport. Earlier observations[M.A. Pedrosa et al., Phys. Rev. Lett. 100, 215003 (2008)] of enhanced long-range correlations in the plasma potential through externally induced shear flows in TJ-II stellarator were verified. The newly measured correlation of zonal vorticity and Reynolds stress at induced flow shear indicates an enhancement of zonal-flow drive (copyright 2010 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  9. Determinants of initial utilization of community healthcare services among patients with major non-communicable chronic diseases in South China.

    Directory of Open Access Journals (Sweden)

    Huajie Yang

    Full Text Available BACKGROUND: Although expected to act as gate-keeping primary care providers, as community health service (CHS facilities are severely under-utilized; Chinese people in both rural and urban areas used predominantly higher-tier facilities for primary care purpose, with significant financial and outcome consequences. This study intends to explore the determinants of initial utilization of CHS among patients with major non-communicable chronic diseases (NCDs in order to understand the care-seeking behavior among urban and rural residents in South China. METHODS: A multi-stage cluster random sampling methodology was adopted to create a sample of 19,466 adults with NCDs from 7,970 urban households and 32,035 adults with NCDs from 3,860 rural households in Guangdong, China. Interviews and physical examinations were conducted in 2010 to collect data on patient characteristics, medical conditions, and awareness and utilization of healthcare. Descriptive analysis and logistic regression analysis were performed to study utilization patterns and the factors associated with the patterns. RESULTS: Prevalence of major NCDs in urban areas was significantly higher than that in rural areas (12.55% vs. 8.70%; p<0.001. Second-tier district hospitals were most preferred for initial consultation (46.05% in rural areas vs. 45.32% in urban areas; p<0.001, followed by tertiary general or specialized hospitals (28.39% in rural areas vs. 33.89% in urban areas; p<0.001. The proportion of patients who had initial use of CHS was relatively low (25.56% in rural areas vs. 20.79% in urban areas; p<0.001. Awareness of self-care and the presence of medical insurance were leading factors associated with first contact of CHS facilities in both urban and rural areas. CONCLUSION: The study suggests that CHS facilities are not often used as the first contact for patients in both rural and urban areas in south China. Much effect must be made to enhance the gatekeeper system and improve

  10. Job Analysis on Posts Characteristics in Community Health Service Organizations%社区卫生服务机构岗位特征工作分析研究

    Institute of Scientific and Technical Information of China (English)

    彭迎春; 苏宁; 何永洁; 梁万年

    2011-01-01

    目的 了解社区卫生服务机构医、护、防各类岗位人员目前实际开展的工作内容,各岗位人员所感受的工作压力,对所在岗位应具备的能力评价、培训要求及对所在岗位绩效考核要素的建议.方法 利用,在北京市分层随机抽取7个区县51家社区卫生服务机构的全科医生147名、社区护士93名、预防保健人员76名进行访谈.结果 各岗位人员均认为不仅需要具备岗位所必需的专业知识与操作技能,同时还需要具备沟通能力、协调能力、团队合作能力等较高的素质要求.各岗位人员均感觉工作存在一定的压力,且均有一定的培训需求.结论 只有科学、合理地进行工作分析,才能够制定出真正反映社区卫生服务机构各岗位特征的绩效考核指标.%Objective To explore the working content, pressure, ability, demand for training of general practitioners , community nurses, and prevention physicians in community health services ( CHS ) organizations, and key elements of post performance appraisal for them were proposed. Methods Totally 147 general practitioners , 93 community nurses and 76 prevention physicians from 51 CHS organizations of 7 districts or counties of Beijing were enrolled by stratified sampling, and they were interviewed by using Job Analysis Questionnaire for CHS Personnel. Results CHS personnel of the above posts held that not only professional knowledge and skills were indispensable , but also the higher qualities of ability of communication , collaboration and teamwork were necessary. They felt a certain pressure on working and all had demand for professional training. Conclusion The performance appraisal indicators of each post reflecting its post characteristics in CHS organizations can be made only on the basis of scientific and reasonable job analysis.

  11. Knickkopf and retroactive proteins are required for formation of laminar serosal procuticle during embryonic development of Tribolium castaneum.

    Science.gov (United States)

    Chaudhari, Sujata S; Noh, Mi Young; Moussian, Bernard; Specht, Charles A; Kramer, Karl J; Beeman, Richard W; Arakane, Yasuyuki; Muthukrishnan, Subbaratnam

    2015-05-01

    Chitin, a homopolymer of β-1-4-linked N-acetylglucosamine synthesized by chitin synthase A (Chs-A), is organized in the procuticle of the postembryonic cuticle or exoskeleton, which is composed of laminae stacked parallel to the cell surface to give stability and integrity to the underlying insect epidermal and other tissues. Our previous work has revealed an important role for two proteins from Tribolium castaneum named Knickkopf (TcKnk) and Retroactive (TcRtv) in postembryonic cuticular chitin maintenance. TcKnk and TcRtv were shown to be required for protection and organization of newly synthesized procuticular chitin. To study the functions of TcKnk and TcRtv in serosal and larval cuticles produced during embryogenesis in T. castaneum, dsRNAs specific for these two genes were injected into two week-old adult females. The effects of dsRNA treatment on ovarial integrity, oviposition, egg hatching and adult survival were determined. Insects treated with dsRNA for chitin synthase-A (TcChs-A) and tryptophan oxygenase (TcVer) were used as positive and negative controls for these experiments, respectively. Like TcChs-A RNAi, injection of dsRNA for TcKnk or TcRtv into adult females exhibited no adult lethality and oviposition was normal. However, a vast majority of the embryos did not hatch. The remaining (∼10%) of the embryos hatched into first instar larvae that died without molting to the second instar. Chitin content analysis following TcKnk and TcRtv parental RNAi revealed approximately 50% reduction in chitin content of eggs in comparison with control TcVer RNAi, whereas TcChs-A dsRNA-treatment led to >90% loss of chitin. Furthermore, transmission electron microscopic (TEM) analysis of serosal cuticle from TcChs-A, TcKnk and TcRtv dsRNA-treated insects revealed a complete absence of laminar organization of serosal (and larval) procuticle in comparison with TcVer dsRNA-treated controls, which exhibited normal laminar organization of procuticular chitin. The

  12. Clinical, laboratory and molecular signs of immunodeficiency in patients with partial oculo-cutaneous albinism.

    Science.gov (United States)

    Dotta, Laura; Parolini, Silvia; Prandini, Alberto; Tabellini, Giovanna; Antolini, Maddalena; Kingsmore, Stephen F; Badolato, Raffaele

    2013-10-17

    Hypopigmentation disorders that are associated with immunodeficiency feature both partial albinism of hair, skin and eyes together with leukocyte defects. These disorders include Chediak Higashi (CHS), Griscelli (GS), Hermansky-Pudlak (HPS) and MAPBP-interacting protein deficiency syndromes. These are heterogeneous autosomal recessive conditions in which the causal genes encode proteins with specific roles in the biogenesis, function and trafficking of secretory lysosomes. In certain specialized cells, these organelles serve as a storage compartment. Impaired secretion of specific effector proteins from that intracellular compartment affects biological activities. In particular, these intracellular granules are essential constituents of melanocytes, platelets, granulocytes, cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Thus, abnormalities affect pigmentation, primary hemostasis, blood cell counts and lymphocyte cytotoxic activity against microbial pathogens. Among eight genetically distinct types of HPS, only type 2 is characterized by immunodeficiency. Recently, a new subtype, HPS9, was defined in patients presenting with immunodeficiency and oculocutaneous albinism, associated with mutations in the pallidin-encoding gene, PLDN.Hypopigmentation together with recurrent childhood bacterial or viral infections suggests syndromic albinism. T and NK cell cytotoxicity are generally impaired in patients with these disorders. Specific clinical and biochemical phenotypes can allow differential diagnoses among these disorders before molecular testing. Ocular symptoms, including nystagmus, that are usually evident at birth, are common in patients with HPS2 or CHS. Albinism with short stature is unique to MAPBP-interacting protein (MAPBPIP) deficiency, while hemophagocytic lymphohistiocytosis (HLH) mainly suggests a diagnosis of CHS or GS type 2 (GS2). Neurological disease is a long-term complication of CHS, but is uncommon in other syndromic albinism. Chronic

  13. 基于几丁质酶基因序列分析鉴定兔源须癣毛癣菌%Identification of Trichophyton mentagrophytes in Rabbit based on the Chitinase Gene Sequence Analysis

    Institute of Scientific and Technical Information of China (English)

    刘月; 刘彦威; 白福娟; 刘娜; 刘伯

    2015-01-01

    利用几丁质酶基因(CHS1)序列分析和形态学方法对兔源须癣毛癣菌分离株(31株)进行鉴定和分型.从患兔采集病料,在沙保弱培养基进行分离培养,观察菌落和菌丝、孢子的形态及尿素酶、毛发穿孔等生理试验表现;提取病料和分离株DNA,扩增目的基因,通过序列分析,结合形态学方法对分离株进行鉴定,根据分离株序列同源性差异进行基因分型.结果显示,分离株的菌落多数为颗粒型和粉末型,占87.09%(27/31),背面有黄褐色素沉着,有大量葡萄状小分生孢子、螺旋菌丝、梳状菌丝及棒状大分生孢子,尿素酶试验和毛发穿孔试验阳性;CHS1序列比对显示分离菌株与须癣毛癣菌复合体的指间须癣毛癣菌有99.2%~95.4%相似性.根据CHS1序列比对结果,结合分离株的形态学特征,将该菌株鉴定为亲动物指间须癣毛癣菌.基于CHS1序列同源性差异,将分离株分成5种基因类型,其中Ⅰ型和Ⅱ型是优势菌株,占54.84%(17/31),提示CHS1序列分析可以用于须癣毛癣菌的鉴定和基因分型.

  14. Operationalizing frailty among older residents of assisted living facilities

    Directory of Open Access Journals (Sweden)

    Patten Scott B

    2011-05-01

    Full Text Available Abstract Background Frailty in later life is viewed as a state of heightened vulnerability to poor outcomes. The utility of frailty as a measure of vulnerability in the assisted living (AL population remains unexplored. We examined the feasibility and predictive accuracy of two different interpretations of the Cardiovascular Health Study (CHS frailty criteria in a population-based sample of AL residents. Methods CHS frailty criteria were operationalized using two different approaches in 928 AL residents from the Alberta Continuing Care Epidemiological Studies (ACCES. Risks of one-year mortality and hospitalization were estimated for those categorized as frail or pre-frail (compared with non-frail. The prognostic significance of individual criteria was explored, and the area under the ROC curve (AUC was calculated for select models to assess the utility of frailty in predicting one-year outcomes. Results Regarding feasibility, complete CHS criteria could not be assessed for 40% of the initial 1,067 residents. Consideration of supplementary items for select criteria reduced this to 12%. Using absolute (CHS-specified cut-points, 48% of residents were categorized as frail and were at greater risk for death (adjusted risk ratio [RR] 1.75, 95% CI 1.08-2.83 and hospitalization (adjusted RR 1.54, 95% CI 1.20-1.96. Pre-frail residents defined by absolute cut-points (48.6% showed no increased risk for mortality or hospitalization compared with non-frail residents. Using relative cut-points (derived from AL sample, 19% were defined as frail and 55% as pre-frail and the associated risks for mortality and hospitalization varied by sex. Frail (but not pre-frail women were more likely to die (RR 1.58 95% CI 1.02-2.44 and be hospitalized (RR 1.53 95% CI 1.25-1.87. Frail and pre-frail men showed an increased mortality risk (RR 3.21 95% CI 1.71-6.00 and RR 2.61 95% CI 1.40-4.85, respectively while only pre-frail men had an increased risk of hospitalization (RR 1

  15. Fibrin-genipin annulus fibrosus sealant as a delivery system for anti-TNFα drug

    Science.gov (United States)

    Likhitpanichkul, Morakot; Kim, Yesul; Torre, Olivia M; See, Eugene; Kazezian, Zepur; Pandit, Abhay; Hecht, Andrew C

    2015-01-01

    Background context Intervertebral discs (IVD) are attractive targets for local drug delivery because they are avascular structures with limited transport. Painful IVDs are in a chronic inflammatory state. While anti-inflammatories show poor performance in clinical trials their efficacy treating IVD cells suggests that sustained, local drug delivery directly to painful IVDs may be beneficial. Purpose To determine if genipin crosslinked fibrin (FibGen) with collagen type I hollow spheres (CHS) can serve as a drug delivery carrier for the anti-TNFα drug, infliximab. Infliximab was chosen as a model drug because of the known role of TNFα in increasing downstream production of several pro-inflammatory cytokines and pain mediators. FibGen was used as drug carrier because it is adhesive injectable, slowly degrading hydrogel with potential to seal annulus fibrosus (AF) defects. CHS allow simple and non-damaging drug loading and could act as a drug reservoir to improve sustained delivery. Study Design/Setting Biomaterials and human AF cell culture study to determine drug release kinetics and efficacy. Methods Infliximab was delivered at low and high concentrations using FibGen with and without CHS. Gels were analyzed for structure, drug release kinetics, and efficacy treating human AF cells following release. This work was funded by grants from the NIAMS/NIH (R01 AR057397), AO Foundation, and from the Icahn School of Medicine at Mount Sinai. Results Fibrin showed rapid infliximab drug release but degraded quickly. CHS alone showed a sustained release profile but the small spheres may not remain in a degenerated IVD with fissures. FibGen showed steady and low levels of infliximab release that was increased when loaded with higher drug concentrations. Infliximab was bound in CHS when delivered within FibGen and was only released following enzymatic degradation. The infliximab released over 20 days retained its bioactivity as confirmed by the sustained reduction of IL-1

  16. 黄鳝骨硫酸软骨素多糖降血脂功能研究%Study on lipid-decreasing effect of Chondroitin Sulfate Polysaccharides from monopterus albus bone

    Institute of Scientific and Technical Information of China (English)

    姚晓燕

    2011-01-01

    目的:观察黄鳝骨硫酸软骨素多糖(Chondroitin Sulfate Polysaccharides,CHS)对高脂小鼠的降血脂功能.方法:将经预处理后的黄鳝骨经碱提、酶解、过柱(大孔吸附树脂)、脱盐得到CHS,灌胃给高脂小鼠,研究其降血脂功能.结果:多糖低、中剂量组(9、27 mg/kg)与高脂模型组比较,能显著降低高脂小鼠血清总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)水平(P<0.01),对血清三酰甘油(TG)水平升高也有一定的抑制作用,但差异不显著;而高剂量组(81 mg/kg)仅能显著降低血清TC水平(P<0.05);各组对小鼠体重和腹部脂肪均影响不大.结论:27 mg/kg的CHS能显著降低高脂小鼠TC和LDL-C水平,并在一定程度上抑制小鼠TG水平的升高,具有一定降血脂功能.%Objective: To study the lipid-decreasing effect of Chondroitin Sulfate Polysaccharides (CHS), which was extract ed from monopterus albus bone on hyperlipidermia mice. Methods: CHS was extracted from pretreated monopterus albus bones by alkali-liquor extraction, enzyme decomposition, column separation and desalination. Then it was used on hyperlipidermia mice to observe the function of lipid-decreasing. Results: The total serum cholesterol (TC) and low-density lipopro-tein (LDL-C) were very significantly decreased (P<0.01) in the low and middle-dosage groups (9, 27 mg/kg) compared with hyperlipidermia model group, and the rising of triglyceride (TG) was also be inhibited a little, but without distinct diversity; the TC level of the high-dosage group (81 mg/kg) could be obviously decreased (P<0.05); there had no effects on the weight and belly fat of mice of each groups. Conclusion: CHS (27 mg/kg) can decrease the TC and LDL-C level of hiperlipemia mice, inhibite the raisen of TG in a certain degree, and show some colesterol-lowering function.

  17. Survey and analysis of the status of the community health service in Qinhuai district of Nanjing%南京市秦淮区社区卫生服务现状调查和分析

    Institute of Scientific and Technical Information of China (English)

    贺勤; 陈宇炼

    2012-01-01

    目的 调查南京市秦淮区社区卫生服务(CHS)现状,为制定与CHS相适应的策略提供依据.方法 采用访谈法、问卷调查法了解南京市秦淮区CHS现状.结果 南京市秦淮区CHS中心机构健全,用房面积、医生数量符合“基本标准”要求;但护士数量偏少.医护人员专业结构基本合理,持证情况良好;但医护人员学历、职称普遍偏低.近三年总的财务收支基本平衡,略有盈余;政府对CHS中心经费投入仅占年总收入的11.36%,药品收入占总收入的55.40%.CHS中心大部分具备基本医疗设备,但仍有少数CHS中心缺乏一些重要的常规医疗设备如简易手术设备、B超机、血球计数仪、尿常规分析仪、生化分析仪等.就诊患者满意度较高.结论 南京市秦淮区CHS现状基本上是好的.政府应加大对CHS中心的财政投入,进一步加强卫生服务队伍的建设.%Objective To conduct a survey on the status of the community health service(CHS) of Qinhuai district of Nanjing city for providing a basis in the establishment of reasonable CHS strategies. Methods The CHS status of Qinhuai district of Nanjing China was investigated by interview and questionnaire survey. Results Organizational structures of the CHS centers in Qinhuai district were of healthiness with the construction areas and the numbers of physicians which' fulfiled the basic standards except for relatively less number of nurses. Professional structure of the medical care personnel was basically reasonable with formal certificate qualifications, but a common fact of relatively lower education background and professional titles existed. In the recent three years, total financial income and expenditure of these centers were generally balanced with slight surplus. Of total income, the financial support from government accounted for 11.36%, the income from drugs accounted for 55. 40%. Most of the centers had necessary medical equipments, but some centers

  18. Division of Layers in Concrete and Characterization Methods for Distribution of Chloride Concentration and Its Application%混凝土层划分与氯离子分布的表征方法及应用

    Institute of Scientific and Technical Information of China (English)

    黄智山; 魏荟荟

    2013-01-01

    Study and evaluation of permeability of concrete surface are related to quantization of the secondary area of the concrete surface layer and its performance characterization.Some parameters relating to concrete layers are studied.Three main concepts including the fault displacement from the inner surface x,the median of fault displacement from the inner surface z,and the unit fault layer from surface Z is proposed.Then the division method,characterization method and distribution model of unit cross-section layer S as well as the performance parameter and the distribution characterization method of the unit crosssection layer are established.The research purpose and significance of permeable surface layer based on chloride ion are clarified.The test result of chloride ion mass fraction in unit cross-sectional layer is analyzed with the characterization methods of S and its CHS.It is known that the surface layer thickness affecting the permeability of concrete is 2.0-3.0 mm.Z3 =2.5 ± 0.5 is a special fault.The fault magnitude of stratified sampling is sub-millimeter level.The thickness of permeable surface layer is limited to 10 mm.The apparent distribution model of CHS is an exponential function CHS,b-z(x).The benchmark of CHS at the inner surface of permeable layer surface is different from that at outer surface.The distribution model of the position and distribution of fault as well as the structure layer partly relating to the layers are proposed.At the same time the characterization method for CHS and its distribution are put forward.%混凝土表面渗透性的研究与评价,均与表面层的次级层量化及其性能表征有关.阐述了混凝土中部分与层有关的参数,提出距表面断距x、距表面单位断距中值z和距表面单位断距层Z这3个主要概念,建立了混凝土中层的划分方法、表征方法及其分布模型与单位横断层性能参数CHS(单位横断层氯离子质量分数)及其分布的表征方法,通过该

  19. Rod internal pressure quantification and distribution analysis using Frapcon

    Energy Technology Data Exchange (ETDEWEB)

    Jessee, Matthew Anderson [ORNL; Wieselquist, William A [ORNL; Ivanov, Kostadin [Pennsylvania State University, University Park

    2015-09-01

    This report documents work performed supporting the Department of Energy (DOE) Office of Nuclear Energy (NE) Fuel Cycle Technologies Used Fuel Disposition Campaign (UFDC) under work breakdown structure element 1.02.08.10, ST Analysis. In particular, this report fulfills the M4 milestone M4FT- 15OR0810036, Quantify effects of power uncertainty on fuel assembly characteristics, within work package FT-15OR081003 ST Analysis-ORNL. This research was also supported by the Consortium for Advanced Simulation of Light Water Reactors (http://www.casl.gov), an Energy Innovation Hub (http://www.energy.gov/hubs) for Modeling and Simulation of Nuclear Reactors under U.S. Department of Energy Contract No. DE-AC05-00OR22725. The discharge rod internal pressure (RIP) and cladding hoop stress (CHS) distributions are quantified for Watts Bar Nuclear Unit 1 (WBN1) fuel rods by modeling core cycle design data, operation data (including modeling significant trips and downpowers), and as-built fuel enrichments and densities of each fuel rod in FRAPCON-3.5. A methodology is developed which tracks inter-cycle assembly movements and assembly batch fabrication information to build individual FRAPCON inputs for each evaluated WBN1 fuel rod. An alternate model for the amount of helium released from the zirconium diboride (ZrB2) integral fuel burnable absorber (IFBA) layer is derived and applied to FRAPCON output data to quantify the RIP and CHS for these types of fuel rods. SCALE/Polaris is used to quantify fuel rodspecific spectral quantities and the amount of gaseous fission products produced in the fuel for use in FRAPCON inputs. Fuel rods with ZrB2 IFBA layers (i.e., IFBA rods) are determined to have RIP predictions that are elevated when compared to fuel rod without IFBA layers (i.e., standard rods) despite the fact that IFBA rods often have reduced fill pressures and annular fuel pellets. The primary contributor to elevated RIP predictions at burnups less than and greater than 30 GWd

  20. 双色鸳鸯美人蕉中花青素合成关键基因的克隆与表达分析%Cloning and Expression Analysis of Anthocyanin Biosynthesis Genes from Red-Yellow Flower Chimera in Canna ge ne ralis

    Institute of Scientific and Technical Information of China (English)

    李军; 高广春; 李白

    2015-01-01

    The red-yellow flower chimera in Canna ge ne ralis (Canna ge ne ralis Bailey) was used as material and the chalcone synthase gene (CgCHS) and dihydroflavonol 4-reductase gene (CgDFR) were cloned using RT-PCR. The 549 bp and 570 bp fragment of CHS and DFR genes were cloned, sequence comparison showed that these genes share 70%to 95%identities with each previously reported corresponding genes. The expression patterns of CHS, DFR and CHI (chalcone isomerase) genes were analyzed by Real-time quantitative PCR (RT-qPCR). The results showed that the expression quantity of CHS and DFR genes was higher in red-part than in yellow-part, while CHI gene showed on the contrary. The expression of CHS and CHI genes increased in accordance with flower development in red-part, while in the yellow-part its expression increased in the earlier three developmental stages and then reduced in the fourth stage. The expression pattern of DFR gene was consistent with flower development both in the red- and yellow-parts. The expression pattern of these three genes in Canna ge ne ralis varied with different flower color were different with each other.%本研究以双色鸳鸯美人蕉为试材,采用RT-PCR技术扩增查尔酮合成酶基因(CHS)及二氢黄酮醇4-还原酶基因(DFR)序列,并利用RT-qPCR技术检测CHS、DFR及查尔酮异构酶基因(CHI)在双色鸳鸯美人蕉同一朵花不同颜色部位、不同发育时期的表达差异以及在不同花色美人蕉中的表达特性。结果显示:通过扩增分别获得了549 bp的CHS基因片段及570 bp的DFR基因片段,与红掌、百合等相应基因的同源性达70%~95%。基因表达分析表明,CHS及DFR在红色花瓣中的表达量高于黄色花瓣,CHI在黄色花瓣中的表达量高于红色花瓣;CHS与CHI在红色花瓣中随着发育表达量逐渐增大,在黄色花瓣随发育表达量呈先增加后减少的趋势;DFR在红色及黄色花瓣的发育后期表达量逐渐增大;在不同